LOCUS       DASREK010000001        18154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_494440, whole genome shotgun sequence.
ACCESSION   DASREK010000001 DASREK010000000
VERSION     DASREK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 18154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18154
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494440"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFA54_00005"
     CDS             <1..197
                     /locus_tag="VFA54_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZS49212.1"
                     /translation="TKLFPYEWYDSPNIHFLTVEDFEQLAAAETLEVERRIFLSADRR
                     VRLLPNLRAQVAVYLLKKKS"
     gene            complement(289..954)
                     /locus_tag="VFA54_00010"
     CDS             complement(289..954)
                     /locus_tag="VFA54_00010"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HZS49213.1"
                     /translation="MKIGNDAYLRQLVKFVPSETPGMSHQATHWKVLVSRIKRRNAPF
                     VQDGPFQGERPPGRQSALLLVDLFFPAPAGIRHIGFEDMMSGVPAELQAVAAERFITE
                     PSRQAAFRHGIDLFNHGRFFECHEVLEEIWTPCRGPERLFLQALIHFAVGFYHHQRKN
                     DEGAARQLRKGLKKLEAYLPQFEKIDTARLSAQIRRRLRALETGGRIQKFPKIQRLQA
                     RRR"
     gene            880..1325
                     /locus_tag="VFA54_00015"
                     /pseudo
     CDS             880..1325
                     /locus_tag="VFA54_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            1407..1832
                     /locus_tag="VFA54_00020"
     CDS             1407..1832
                     /locus_tag="VFA54_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49214.1"
                     /translation="MFRRFFQRRPALPTSVPGPLHGAPAVQRQKTYSAQTGYVYQYFY
                     QGYRGSERAGQGGNEYVFLVTSDRKSSFPLTIFLPASAVQLWQRDHGRTLTATEQYAA
                     VKMALFEAFDERASLGPADAEVELAAADVERHLATLDID"
     gene            complement(1966..2469)
                     /locus_tag="VFA54_00025"
     CDS             complement(1966..2469)
                     /locus_tag="VFA54_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZS49215.1"
                     /translation="MQEITAVFIHASRKAPATWLERIQKCVDELTTEQIWWRGQENSN
                     AIGNLMLHLSGNVRQWIVSGVGGAPDTRNRNAEFAQRDAVPAAELLQRLRAAVADADQ
                     VLASLTAENLLERRQIQKYDVTVLEAIHHVTEHFALHAGQIIYATKLLTGQDLGFYRH
                     LQQSGTK"
     gene            2532..2945
                     /locus_tag="VFA54_00030"
     CDS             2532..2945
                     /locus_tag="VFA54_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49216.1"
                     /translation="MGFLDNLENDLKNLESAAEHDSAERARQQKAREAEQASARAASP
                     HAHELRNSAFTAELLNRASTIGRSRRLNVRVMWIGSTLRLQAGSRLLELRPTPSGVRA
                     HFLHGNTELESAAVDFSSNPEILARRWLDSITAGS"
     gene            3118..4071
                     /locus_tag="VFA54_00035"
     CDS             3118..4071
                     /locus_tag="VFA54_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZS49217.1"
                     /translation="MRSMGTERRRLQRTSQFQVQELKLYRYEGTEARQVITARLINMN
                     EGGMGIETAVPLEAGLFVAVEGEIVAGGACRRVKGRARVAYCLSLANHLYRVGLNLNG
                     GEAQDSSTQGAHLSSQKEPFVDYYEVLQLSPNADLETIHRVYRALAQRYHPDNRESGD
                     EEKFKLLLKAYRVLSDPEQRRAYDIEHKAAEPRRTKVLEWPKRPPDVNVEKNKRRKLL
                     SLLYLKRISDPEQPSLTTLEMEEMLGITREQLQFSLWYLRESGWIVRGDNGRHFITAK
                     GVDQAESEGALQFRDEKLISTSGQTLRPETFEAGRDSSLVV"
     gene            complement(4103..5266)
                     /locus_tag="VFA54_00040"
     CDS             complement(4103..5266)
                     /locus_tag="VFA54_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49218.1"
                     /translation="MSADSFERVLETLESGGPEAGFEYLAQKFREEKNYPLLFETRLM
                     RARHELGLPLLDRQATSTLPPDLLRIYDERLREAARETGSLFLADGDVVRAWPYFHAI
                     GDRAPVAEAIERLEPEKGTAQIIEIAYQQGVNPRKGFEMVLANFGICRAIQMFSQYPG
                     REGRDHCIRLLVRSLYGDLVQGLKWTIEQREGRAPESQSVPDLLAGRDWLFEGDSYYV
                     DCSHLISVLRFSLETDDPETLRLAIEMADYGTRLSPTFQYPGDPPFEDVYRDHGVYLR
                     ALLGEQIEEAIAHFRSKTEKGDAHSRSAAAQALVALLAHLGRHREAIELSLAHLADAS
                     SYELSCPSILELCEMAGDYGQLMTFARERGDLLSFLAAAIEAPVAAGGRPGRN"
     gene            5569..6399
                     /locus_tag="VFA54_00045"
     CDS             5569..6399
                     /locus_tag="VFA54_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZS49219.1"
                     /translation="MRLRTVLFAFCASALLLGVVWALDRPFREYPGVEYENFPIPPDY
                     QEQTEWVFARLMYPPVGPRYGGFQFLGDWRLGASNWTMDYPRSDRHFTEALRRLTRVH
                     ARSVEQPVDLNDGDDVFDWPWLYGVEVGHWNLTDAQAAKLREYLLRGGFFMCDDFHGT
                     LEWQIFTASMKRVFPDRQIVEIESRDPIFHILYDLNDRFQVPGAQFLRSGRTYEYDGV
                     VPHWRGIYDDKGRLMVAICFNMDLGDSWEHADNPEYPEKFTSLGVRLGVNYVLYSMTH
                     "
     gene            complement(6528..6683)
                     /locus_tag="VFA54_00050"
     CDS             complement(6528..6683)
                     /locus_tag="VFA54_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49220.1"
                     /translation="MPKDADIVPEGAAAPSSAEKPPSQGSEAEEREIGDFITRQVLEK
                     GSVFFDG"
     gene            6838..7431
                     /locus_tag="VFA54_00055"
     CDS             6838..7431
                     /locus_tag="VFA54_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS49221.1"
                     /translation="MRYVVTGGNGFFGRILVECLLRRGEEVLAIDRLADPSAPAGVRF
                     ARADLRDFDRLYQRLREFQPIDGVFHVAAILAHEKKDRFMLWDSNVHGTGNLLGACVR
                     LGVPKLVFTSSNCIFGRGSKSPVDESAPPHPVEIYGRSKLEAERRIVANGPRLPYSIV
                     RCPTIVGAGRLGLLAILFDFIRENRRVYLVGNGENRY"
     gene            7522..7878
                     /locus_tag="VFA54_00060"
     CDS             7522..7878
                     /locus_tag="VFA54_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49222.1"
                     /translation="MPTIAESYRRLIEHARSGSQLISVPRAAALSLMRMAYWIGASPL
                     GPYHYRMIAENFVFDAARIKRTLGWTPTLTNSEMLIEAYDWYLHNYGRICRASAQSAH
                     RSPAPQGIISLLRALS"
     gene            8032..9246
                     /locus_tag="VFA54_00065"
     CDS             8032..9246
                     /locus_tag="VFA54_00065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS49223.1"
                     /translation="METALALHAPFWRDEAQTIFIARLPHWTDVIEALARDGGAPLFY
                     FLLRHWMRVAGSGAGIRWLPILLSSVAPAVIFLVGENLAGRTAGMTAAVWWLVNPVAV
                     DAGAQVRMYCLTASLGTLTLWAWIRFLGNGRLRCGLAGAVGSVALLYTHNTGIVLLAT
                     AQIGWLVWAVAARRRKALDAGLMLRWAMLQAAIGLAYLPWAPVLLRQISYDVTPWAGG
                     IGIQRMIVNGNCFFPVPVLSTAAPLAMMAAAVVAGVTLYVFQKPEMGWLRPIFTLPVA
                     GIASLAAATYFRGAFGPRYILPWVPMLALGVFAFTGTVLRKPLRRLVVAVLIACQAIG
                     LIFAMNRIAKPTNAPEIGRFLMRSPAGEENIIVMPWEAGAPSISLYLPPQAGEEIGSR
                     RNAAWRSSTGPF"
     gene            9219..9545
                     /locus_tag="VFA54_00070"
     CDS             9219..9545
                     /locus_tag="VFA54_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49224.1"
                     /translation="MEVINWAFLKNRLSDDRAFAAFLHRIGAEARRHKTLWLICQTSA
                     LESSPVQQSQTFVNLERSRERDTIIWLEGVAGPPAVQLRGCDCGYESFALLRFDSRLH
                     ADLPEH"
     gene            9700..10662
                     /locus_tag="VFA54_00075"
     CDS             9700..10662
                     /locus_tag="VFA54_00075"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HZS49225.1"
                     /translation="MSLRRAGLVIVCFAAISLAQQRDIPDLGYKAVPDWPDLPAGWNF
                     GEVASVAADSHGHVLVFHRGPHPIMEFDSGGKFVRSWGDGMISTSPGPNNPTYALVPP
                     RPPCDSCGAHSVRLDPDGNIWVVDDGGHVVIKMNPQGRVVMELGRRGVPGSGHANFNL
                     PTDVAFAPNGDFYVTDGYGNSRVVKFARDGRYLLEWGTRGSGPGEFLLPHAVVLDAQG
                     RVYVADRESRRIEIFDSDGKFLRQWGNIGAFSGLAITGDQRIWAAGGGNVVLLNLEGQ
                     ILGKLAPSGKLPGQVDAAHGIAVTTGGDVYVAELNWRVQKFVKK"
     gene            complement(10681..11646)
                     /locus_tag="VFA54_00080"
     CDS             complement(10681..11646)
                     /locus_tag="VFA54_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49226.1"
                     /translation="MRRRFILIIALAFALAETCFSGDEPSPQDPAAREQMIREMKELE
                     KALGFRRSGNFLNSSDKIAAYYRCYYTGKLEIPSSYDDLQLKQTNQPACEVDSEKYDV
                     FFYPIEAVANGKSPVTTSLQNSTMERLLVVVPHEDFHVHKNSEKLPASVTEAASTLIG
                     FLTAIEFAQQKFGMASPVYQNLSKEPELFLRKAEVVNRYYPEFKMLYASARAGRIGRE
                     EALAAKQNLFARMEEECGAITPDPTSFNKCLAAANNAGLAFDYTYTKNYPLLYELFLA
                     KDRQMRPTIEAIERVLAAQPSSEEAAIGAVRNAIEEQKRPASGPN"
     gene            complement(12075..12752)
                     /locus_tag="VFA54_00085"
     CDS             complement(12075..12752)
                     /locus_tag="VFA54_00085"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS49227.1"
                     /translation="MKRMLAMFTVLAVGVVLAAPPGAAPNTLTAKEKAEGWILLFDGK
                     TLDNWDPNTNPNGAWKVVDGTIMTDSPRGTTLLTKEDFANFELRLEFRTTDNVNSGVM
                     LRNPRPHPAPPGEAKGKKKGGGQGYEVQIRDKDPGHYTGGSYLTASLVNVQKAPADAK
                     IRNGEWNTFDITADGDHFVIVYNGQKVVDAHDSKLANGAIGLQWAHPEDAPGTKIEFR
                     NIRVKRL"
     gene            12931..13698
                     /locus_tag="VFA54_00090"
     CDS             12931..13698
                     /locus_tag="VFA54_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49228.1"
                     /translation="MRKVLLFVLAATFGVAAWADTLIMRDGARYTGTLIDGNSRQVTF
                     ADQNGMRRTFNTSEIQAVEFQSGAYSQGQTGGVQMGAALLPAGTQIAVRTNENIDSTS
                     AATGRTYSATITRDVLDQNGNVVIPNGSPAQLIVRNISSGGTFGSAELALDLQSVDVN
                     GQRYTVSTADVQQQGAGGLGANKRTGEYVGGGALLGTVLGAIAGGGKGAAIGALGGAA
                     AGAGVQVLTRGKEVHVPAETQLTFRLDQPLQLQPMRR"
     gene            complement(13750..14373)
                     /locus_tag="VFA54_00095"
     CDS             complement(13750..14373)
                     /locus_tag="VFA54_00095"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS49229.1"
                     /translation="MRRILAATLTIGLWTALAVGPEAPNTLTPQETAQGWKLLFDGKT
                     LNGWTPHGAAEWKIVDGAVTTNSPKGGALYSNEDYDNFVLKLEFQGTPDINSGVYIRM
                     ARQGNIHDVGYEIQIRDIDEQGYTTGSLVNMAKTASLAKIIPGQWNRMEITAQGDHIT
                     VVYNGKKVLDAHNSLHTTGAIGLQWAHPERAPGKSIAFRNIKVRRLT"
     gene            complement(14468..15154)
                     /locus_tag="VFA54_00100"
     CDS             complement(14468..15154)
                     /locus_tag="VFA54_00100"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS49230.1"
                     /translation="MKRIAFVLGAVGLWAAFAAPPEPPNTLSAKEKSEGWILLFDGHT
                     LNGWTPSSNSAEWKVEDGAIMMHSDKGGNLFTNDTFDNFELQIEFRTTPDVNSGIYLR
                     YTPRPYNPATKGQPKPKGKSQGMPGYEINIRDSDVRPVHDSPGGFDTGSLVGVLKAEK
                     AQIIPGQWNRFDITAQGDHFIVIYNGKKILDGHDAKFASGKIGLQWAHPELVTGKKIE
                     FRNIKVRRLS"
     gene            15367..16389
                     /locus_tag="VFA54_00105"
     CDS             15367..16389
                     /locus_tag="VFA54_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS49231.1"
                     /translation="MNKLGVGVIGVGEMGKRHAENVRYRVPKARLIAVADANPERARS
                     VAAELEIEHFYDNAEDLVARTDIDAVVIGSPPRVHPALIRSAAAAKKHILCEKPLTLT
                     LEEADEVLDAVSKAGVFFQVGHMRRYDPGYAAARQRMEAGEIGEPVLFKSIGRDAAPP
                     PPAYFECGLNGTLFLDSSVHEFDLARWLMADEVVEVHAFAAAKSLPELAKRGAYDSGV
                     VNLRFAGGAIGNTESFMNAGYGYDIRTEIVGTKGALQIGYLGYTPLVVLKPGVSSHDN
                     VQHWLQRFADAYLREMSDFVESVLAGRRPRVTGYDGRQAVAIALAAECSYKEGRTVRL
                     KNPATA"
     gene            complement(16422..17306)
                     /locus_tag="VFA54_00110"
     CDS             complement(16422..17306)
                     /locus_tag="VFA54_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZS49232.1"
                     /translation="MLSLRHILLPTDFSRRSVVAARHAAPLAKHFHSKITLLHVAQVV
                     RLSPGDVQVKRIVVEETLAEHQKQVRAQLHSFLAEELAEFEVNRVLAEGDPAGKIVEY
                     AHSENVDLIMMPTRGWGTFRRFILGSVTAKVLHDVRCPVWTGVHIEEPAEEAAPPVRT
                     VLCAIDLASNVSEHLRWASWFAKEFDAELVIVHAIPSFEFTPETVYLQSELLRLMSSE
                     AKEKVVKMLGECAPPGIQIQQVLAGGSVSKVVRTAAENHGAGLVVIGRASAAGLLGRL
                     RTDAYAIIRDSPCPVISV"
     gene            17555..>18154
                     /locus_tag="VFA54_00115"
     CDS             17555..>18154
                     /locus_tag="VFA54_00115"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HZS49233.1"
                     /translation="MRWRGVVAAFACGVALMPACGQRQPKTRLKRQEFGKMQNGAPVE
                     LFTLTNKSGMEAAVTNYGGILVSLKTPDRNGRLADVVLGFDSLDGYLQPEPYFGAIVG
                     RYANRIGHGTFTLNGVSYTLARNNGENALHGGIKGFDKVVWQAKDVSTDAAPSLELTY
                     LSKDGEEGYPGNLSARVVYTLTDDNELKIDYTATTDKDTV"
BASE COUNT         3395 a         5434 c         5567 g         3758 t
ORIGIN      
        1 aaacaaagct tttcccctac gagtggtatg attcgcccaa tattcacttc ctcactgtcg
       61 aagatttcga gcagttggcc gcggctgaga ctctcgaggt ggagcggcga atctttttga
      121 gcgccgaccg gcgcgtccgc ctgctcccca atctgcgcgc gcaggtcgca gtctatttgc
      181 tgaagaagaa aagctgatct cggccggcgg gctgaactca ggggcgatcg attttgccag
      241 tctttgccgc ctgccgtaag cctgaggctc ccgggagatc tcggtcggct agcgccgccg
      301 ggcttgcagc ctctgaatct tgggaaattt ttggatgcgc ccgcccgtct cgagggctcg
      361 caggcgccgc ctgatctgcg cagagagacg tgctgtatcg attttctcaa actgcggcag
      421 ataagcttcg agtttcttga gtccctttcg cagttggcgc gcggccccct cgtcgttctt
      481 cctctggtga tgatagaagc cgacggcgaa gtggatgagc gcttgcagaa acagccgctc
      541 gggcccacgg cagggagtcc agatctcttc gagcacctcg tggcattcga aaaaccggcc
      601 atggttgaag aggtcgatgc catgccggaa cgcagcctgg cggctgggct cggtgataaa
      661 acgctcggcg gcgacggcct gaagctccgc gggcactccg gacatcatat cttcaaaccc
      721 aatatgtcgg attccggccg gcgcaggaaa gaagaggtct accagcagca aggccgattg
      781 ccgcccgggc ggacgctcac cctgaaatgg cccgtcctgc acgaagggcg cattccgcct
      841 tttgatcctg ctcactagga ctttccagtg cgtggcctgg tggctcatcc ccggagtttc
      901 acttgggacg aatttaacaa gctgccgcag atacgcgtcg ttgccgattt tcactgcgtg
      961 acggcttgga gtaaattcga taatgagtgg acctgcgtgc cgtttcccgc aattgcggaa
     1021 atggcccagc cgctgccgca agcgggatat gtgatggtgc atgccgaccc cggctatgac
     1081 acgaatgtgc cgctcgctga cttgatgcgg ggtaatgtgg tttggcgctt gcgcatgctc
     1141 ccgagccgct tactcccgag cacggcggcc ctctccggct tattgcgccg cacctctatg
     1201 cctggaagag ctccaaatgg ggccgggcgc tcgatttcat ggaacaggat cgcgccggtt
     1261 actgggagag cgtgggttac catatgtatg gagacccttt tcgcgagcag cgctaccggg
     1321 aataaggcgc gtaaacgcgc tggcggcgcg ttgcgcccgc ggccggatcg cgatagtggg
     1381 agcggcccgt gaacggtttc ttctgaatgt ttcgccgatt cttccagcgg cgcccggcgc
     1441 tcccaacaag cgttcccggc cctctgcacg gggcgccggc cgtgcagcgg caaaaaacct
     1501 attccgctca gaccggctac gtctatcagt atttttacca ggggtatcgg ggcagcgaac
     1561 gggcgggcca aggcggcaac gagtacgttt tcctggtgac ttcggaccgg aagtcttcgt
     1621 ttccgctcac gatatttctt cccgcctctg cggtgcaact ctggcagcgg gatcatggcc
     1681 ggacgttgac cgccaccgaa cagtacgcag ccgtcaaaat ggccttgttc gaggctttcg
     1741 atgagcgggc gagcctgggt cctgccgacg ctgaagtgga gctggctgcc gccgacgtgg
     1801 agcgccatct ggcaacgctc gacatcgact gaaaggccgc gagcctgcct ttctccgtgc
     1861 tgctgccgca gccgcccgcc attcgccatt caaccaggag cggcggcgcc cggctgccgc
     1921 gcaagtattg gcgctttccc gtgagaatac cgctgcggcg aagacttatt ttgtcccgct
     1981 ctgctgtaag tgccggtaga aacccaggtc ttggcccgtg agcagcttgg tggcgtaaat
     2041 gatctggccg gcgtgcaggg cgaagtgctc ggtgacatgg tgaatggcct cgagcacggt
     2101 cacatcatac ttctgaatct gccggcgctc aagcaggttc tccgccgtca gcgaagccag
     2161 cacctggtcg gcatcggcca cggccgcgcg caaccgttgg agcagttcgg ctgcggggac
     2221 tgcgtcgcgc tgggcgaatt ccgcgttccg attcctggtg tcgggcgcgc cgccgactcc
     2281 tgaaacaatc cattggcgca cattgccgct caggtgcagc atcagattgc cgatagcgtt
     2341 gctgttttcc tggccgcgcc accaaatctg ctccgtggtg agttcatcga cgcatttctg
     2401 gatgcgctcg agccacgtcg cgggcgcttt gcgagaggca tggataaata ctgccgtgat
     2461 ttcttgcatg atcgcctggc ggaagtcgcc gaaaccgtgg ccgtacgggc ccgagtcatt
     2521 ataatacccg catggggttt ctggacaacc tcgaaaacga tctgaaaaac ctcgaatcgg
     2581 ctgccgagca cgactccgcg gagagagcac gccagcagaa ggctcgcgaa gcggagcaag
     2641 cttcggcgcg ggctgcctct ccccatgccc acgagttgag gaattcggcg ttcacagccg
     2701 agcttctaaa ccgggcttcc accattggcc gcagccggcg cctgaacgtg cgcgtgatgt
     2761 ggatcggctc cacgctgcgg ttgcaggcag gcagccgtct gctggaattg cgtcccaccc
     2821 cgagcggcgt ccgggcgcat tttctgcatg ggaacacgga gcttgaatcg gcggccgtag
     2881 atttttcatc gaacccggag atcttggccc ggcgatggct tgattccatc acagccggct
     2941 cgtgagcgaa agccggatgc caatagaagc ggcccggcgg ctggagacac gccaagataa
     3001 ggtacacgct ttctagggga aataccctgc ctttgaacgg tactccggat tcttccgata
     3061 aaggtactcg tgctccattg tcacccggcc gttctctgcg gcagaatgcg aactgtcatg
     3121 agatccatgg gaacggaaag gcgcagatta caaagaacca gccaatttca agtgcaggaa
     3181 ctaaaactct accgctacga ggggaccgag gcacgccaag tgatcacggc gcggctcatc
     3241 aacatgaatg agggaggcat ggggatcgag accgcggttc cgctggaggc ggggctgttc
     3301 gtcgccgtcg agggcgaaat cgtcgcaggc ggcgcgtgcc ggagggtcaa aggccgggcg
     3361 cgagtcgcct attgcctctc gttggcgaac cacctttatc gcgtaggtct caatttgaac
     3421 ggcggcgagg cgcaagactc ctcgacccag ggcgcccacc tcagctcgca aaaagagcct
     3481 tttgtggatt actacgaggt attacagctc agccccaatg ccgatctcga aaccatacat
     3541 cgcgtctatc gagcgctggc gcagagatac catccggata atcgcgagag cggcgacgag
     3601 gagaaattca agcttctgct aaaggcgtat cgtgtactca gcgaccccga gcagcgccgc
     3661 gcctatgata tcgaacacaa agccgcggag ccccggcgga cgaaggttct ggaatggccg
     3721 aaaaggccgc cggacgtgaa cgtggaaaaa aacaaacggc ggaagctgct ctctcttctc
     3781 tacctgaaga gaatcagcga cccggagcag ccgagcctca ctacgctcga gatggaggag
     3841 atgcttggca tcacccggga gcaattgcaa ttcagcctct ggtacctgcg ggagagcggt
     3901 tggattgtgc gcggggataa tggccgtcac tttatcaccg ccaaaggggt agatcaggcc
     3961 gaatcggaag gagcgctgca attcagggat gaaaagctga tctcgaccag cggccagacg
     4021 ttacgccccg agacgttcga ggcggggcga gactcaagtc tggtagtgtg agctttcgcc
     4081 ctcgccgcac agacggcggc tctcagtttc gtccaggccg cccgcccgca gccaccggcg
     4141 cttcgatggc cgctgccagg aagctcagca agtccccgcg ctcccgggcg aacgtcataa
     4201 gctggccgta atcgcccgcc atctcgcaca attccaaaat cgaagggcag gacaactcat
     4261 agctgctggc gtcggccagg tgcgccagcg agagttcgat ggcctcccga tgcctgccga
     4321 gatgggccag cagagccacg agagcctggg cggccgctga gcgcgagtgg gcgtcgccct
     4381 tctcggtttt cgagcgaaaa tgtgcgatgg cctcttcgat ctgttcgccc agcagcgcgc
     4441 gcagataaac gccgtgatcg cgatagacgt cctcaaacgg cgggtcgccc gggtattgaa
     4501 aagtcggtga aaggcgcgta ccgtaatcgg ccatttcgat ggccagccgt agcgtctccg
     4561 ggtcgtcggt ttccaggctg aaacgcaaca cggagatgag gtgcgaacaa tcaacataat
     4621 agctgtcgcc ctcaaacagc cagtcgcggc cggccagcag atcggggacg ctttgactct
     4681 cgggggcccg gccttcccgc tgctcgattg tccatttcaa tccttgtacc aaatcgccat
     4741 acagcgagcg caccagaagc cggatgcagt ggtcgcgtcc ttcccggccg gggtattggg
     4801 aaaacatttg aatggcgcgg caaattccga aattcgccag caccatctcg aagcctttgc
     4861 ggggatttac cccctgctga taggcaatct cgatgatttg cgccgtgcct ttctcaggct
     4921 ccagccgctc gatggcctcg gccaccgggg cccggtcgcc gatcgcgtgg aaatagggcc
     4981 aggctcgcac aacgtcgccg tcggccagaa acagggaccc cgtctcgcgc gcggcctccc
     5041 ggaggcgctc atcgtagatc cgaagaaggt cgggaggcag ggtggaagtg gcctgcctgt
     5101 caagcaacgg caggccgagt tcgtgccgcg ctctcatcag ccgcgtttca aacaatagcg
     5161 gataattttt ctcttcgcgg aacttttgcg ccagatattc gaagcccgcc tccggaccgc
     5221 cggactccag agtttccagc acccgctcga agctgtcggc gctcatgagg gttcctggtc
     5281 tgcatcgtac cacaagcccg gctggacgcc ggagaggaaa aaggccggcc gttgtggcgc
     5341 gatgcgggcc ctcgcgacgc ttggccgcgc cgtcgcccgg ctcaatccgg acccgccgga
     5401 gaaagctgca aacatggccc cttctttctg ttagcgccgc cggtgcagat ccggcgaagg
     5461 ccttttcccc atcttgccgg cgcctggcag gcgtcccgca accgctacct tcgcagagct
     5521 tggccttgct tgacggaacc cctgggatga gtgctaccgt tgagacagat gcgcctgcgg
     5581 accgttttgt tcgccttctg tgccagcgcc ttgcttctcg gcgttgtctg ggcgctggac
     5641 cggccgttcc gcgagtatcc gggtgttgaa tacgaaaatt ttcccatccc tcctgactat
     5701 caggaacaga cggaatgggt ttttgcccgc ttgatgtatc cgccggttgg cccaaggtac
     5761 ggaggttttc aattcttggg agactggcgc ttgggcgctt ccaactggac catggattac
     5821 ccccgctcgg accggcattt cactgaagct ctccggcgtc tgacgcgggt tcacgcgcgc
     5881 tccgtggagc agcctgtcga tctgaatgac ggcgatgacg tcttcgattg gccgtggctc
     5941 tacggcgtcg aagtggggca ttggaacctg accgacgcgc aggcggctaa attacgcgag
     6001 tatctcctcc gcggcggctt cttcatgtgc gacgatttcc acggcacgct ggagtggcaa
     6061 atcttcaccg ccagcatgaa acgcgtgttc cccgaccgcc agattgtcga gatcgagtcg
     6121 cgcgatccga ttttccacat cttgtacgat ctgaacgatc ggtttcaggt tcccggcgcc
     6181 caattccttc gctccggccg gacctacgag tacgacggag tcgtacccca ctggagaggc
     6241 atctacgacg ataaaggccg cctcatggtg gcgatttgct tcaacatgga tttgggggat
     6301 tcctgggagc acgccgacaa tccggaatat cccgaaaagt ttacttccct cggcgtgcgc
     6361 ctcggcgtca actacgttct ctattccatg acgcattgag cgggctgctg aacaggcaaa
     6421 tccccgctgg ttcgcgctca gctaacggga gcatctccgc tgttcgcgat cccaaggggg
     6481 aagctttctg tgaaaatagg cgtgtaggaa gcagatggca gaacggctta gccatcaaaa
     6541 aagacggagc ctttctccag cacctgccgg gtgataaaat ctccgatctc tctttcctcc
     6601 gcttcgcttc cctgggaagg cggcttttcc gcggaagaag gcgccgctgc cccttcggga
     6661 acaatgtccg catcttttgg catgagaacc ctcgttcatt ctatcaggtt cccaacgatt
     6721 aggtaagatc cagtagaaga gaatccggcg cctgcacggc gcttagccct gctggaactt
     6781 taattagtcc tggcgtgaag ggcgcgattg cgtttatagt ggcttgtctg cgaactcatg
     6841 cgttatgtcg tgaccggcgg caacggattt tttgggcgca tcctggttga gtgcctcttg
     6901 cgccgcggtg aggaagtttt ggcgatcgac cgcctcgccg acccctccgc gccagccggc
     6961 gttcgttttg cacgtgcgga tcttcgagac ttcgatcgtc tctaccagcg cctgcgcgag
     7021 ttccagccga tcgatggagt attccacgtc gctgcgattc tggctcacga gaagaaagac
     7081 cggtttatgt tgtgggattc caatgtgcac gggaccggga atctgctggg cgcctgcgtg
     7141 cggcttggcg ttcctaaact cgtcttcacg agcagcaatt gcatcttcgg ccgcggctcg
     7201 aaatcgcccg tggacgaaag cgccccgcct cacccggtcg agatttacgg acgcagcaag
     7261 ctggaagcgg aaaggcggat tgtcgccaac gggccgcggc tgccgtattc catcgtccgg
     7321 tgcccaacca tagtcggcgc cgggcggctg ggactgctgg ccattctgtt cgacttcatc
     7381 cgcgaaaatc gccgggtcta tctggtgggc aacggagaaa accggtatta gttcgtatac
     7441 gctcaggatc ttgccgcggc ttgtctgctg gcgatgcggc atccaggttc agcccaactt
     7501 ttccacatcg gcagcgattc tgtgccgacg atcgccgaat cttaccgccg gctgatcgaa
     7561 catgcgcgtt ccggcagtca attgatcagt gtgccgcgcg cggcggcgct gtcgctgatg
     7621 agaatggcct attggatcgg cgcttcgccg cttggaccgt atcactatcg gatgatcgct
     7681 gagaactttg tcttcgacgc cgcccgcatc aagaggacgc tcggctggac gcccacgctg
     7741 accaactccg agatgctgat cgaggcttac gattggtact tgcacaatta tggccggata
     7801 tgccgtgcga gcgcgcaatc ggctcaccgc tcgccggcgc ctcaaggcat catctccttg
     7861 ttgagagcgc tttcctgaaa tcaaacaggc tgtgatgcat tgttgaccgg caaacagctc
     7921 tccatccttt tcagccgccc tttgcggcaa ccacagccga tcaatgttcc ggtgcggcag
     7981 ggcagacggc gggtggcgat ttgtgcagcg ctaattgccg tatctatact catggaaacg
     8041 gccctggcgc tgcatgcgcc gttctggcgg gatgaagcgc agacaatctt catcgcccgc
     8101 cttccccact ggaccgatgt gatcgaagcg ttggcgcggg atggcggagc cccgctgttc
     8161 tattttctgc tgcgccactg gatgcgcgta gcgggttccg gcgcgggcat ccgctggctg
     8221 ccgattcttc tttccagtgt cgcgccggcg gtcatttttc tggtgggaga gaacctcgcc
     8281 ggccggactg ccggtatgac ggcagcggtt tggtggctgg tgaacccggt tgccgtggac
     8341 gcgggcgcgc aggtgcgaat gtactgcctc acggcctccc tcggcacgct cactttgtgg
     8401 gcctggatcc gttttctggg caatggccgc ttgcgttgcg ggttggccgg cgccgtggga
     8461 agcgtcgccc ttctatatac tcacaataca ggcatcgttc tgctggcgac agcccaaatc
     8521 gggtggctcg tttgggcagt cgcagcccgc cggcgcaagg ctttggacgc cgggctgatg
     8581 ctgcggtggg ccatgcttca ggcagcgatt gggctggcct atttgccgtg ggctccggtg
     8641 ctgctgcggc aaatctccta tgacgtcaca ccgtgggcag gcggcattgg aatccaaaga
     8701 atgatcgtaa acggaaactg ctttttccct gtgcccgtct tgtctacagc cgctcccctg
     8761 gcgatgatgg cggccgccgt ggtagccgga gtcacgctgt atgtattcca aaaaccggaa
     8821 atgggctggc tgcgtcccat tttcacgctg cctgtggcgg gtatcgcgag cttggccgcc
     8881 gctacgtact tccgaggcgc cttcgggccg cgctacattc tgccctgggt tccgatgctg
     8941 gcgctgggcg tcttcgcttt tacaggcact gtgctccgga agcctctccg ccggctggtc
     9001 gtggccgtgt tgattgcctg ccaggcgatc ggcctgattt tcgcaatgaa ccgcatcgca
     9061 aagccgacaa acgcgcccga aatagggagg tttctgatgc gaagcccggc cggcgaggaa
     9121 aatatcatcg tcatgccctg ggaggcaggc gcgccctcga tcagcctgta tcttcctccg
     9181 caagccggcg aagagatcgg ctcccgacgc aacgccgcgt ggaggtcatc aactgggcct
     9241 ttctgaaaaa ccgcctgagc gatgaccgcg ccttcgcagc gttcttacat agaatcggtg
     9301 ccgaggcccg ccgccacaaa accctgtggc tcatctgcca gaccagcgcc ctggaatcga
     9361 gtccggtgca gcaatcccag acgttcgtga atctggaacg atcgcgggag cgggatacca
     9421 tcatttggct cgagggcgtc gcgggcccgc cggcggtgca gcttcgcggc tgcgattgcg
     9481 gctatgaaag ctttgctttg ctgcggtttg attcgcggct gcacgccgac ctgccggagc
     9541 attgacccgg ccggctgctc tgatcgcgag ccggccacgg tgcgttcgtg aaccgtcaag
     9601 gtttccgctt cgaccgggat ccttgtatta actttcgctt ccgcctttga gccgcggagc
     9661 gcgcgcccgc tataatgcgc gccagggagg aagccctcta tgtctttgcg tcgcgcgggt
     9721 ctggtgatag tttgttttgc cgcaattagc ctcgcccagc agagagacat tccggacctc
     9781 ggctacaagg ccgtgccgga ctggcccgat ctgcccgctg gatggaactt cggcgaagtg
     9841 gcgagcgtgg ccgcggattc ccacgggcac gtgctcgttt ttcaccgcgg tcctcacccg
     9901 attatggaat ttgattccgg ggggaaattt gtacgctcct ggggcgatgg catgatctcc
     9961 acctcccccg gaccgaacaa tccgacatat gctttggtcc cgccccggcc gccctgtgat
    10021 tcctgcggcg cccattcggt gcgcctggac ccggacggca acatctgggt ggtcgatgat
    10081 ggcggccacg tggtgatcaa gatgaatccg caaggccggg ttgtgatgga gctggggcgc
    10141 cggggcgttc ctggctccgg ccatgccaat tttaatctgc ccacggatgt cgccttcgct
    10201 cccaacgggg atttttatgt gaccgatggc tatggaaact cccgtgtggt gaaattcgct
    10261 cgcgacggcc ggtatttgtt ggaatggggg accagggggt cgggcccggg cgaatttttg
    10321 ctgccccacg ccgtggtgct cgatgcgcag ggcagggtgt atgtggctga ccgcgaaagc
    10381 aggcgcatcg agattttcga ctcggacgga aaatttctcc ggcagtgggg gaatatcggc
    10441 gccttctccg ggcttgccat caccggagac cagcgcatct gggcagcggg cggcggcaat
    10501 gttgtcctcc tgaatttgga gggccagata ctgggcaaac tcgcgccttc gggcaaactc
    10561 ccggggcagg tggacgccgc ccatgggatt gcggtcacta ccggtggaga tgtctacgtc
    10621 gccgagctca actggcgcgt gcagaaattc gtgaaaaagt agggcggcgc gggccgcccg
    10681 ctaattcggg ccggatgccg gccgcttttg ctcttcgatc gcgtttcgca ccgcgccaat
    10741 cgccgcctct tccgatgacg gctgggcggc aagcacacgc tcgattgcct cgatggtggg
    10801 ccgcatttgc ctgtcctttg ctagaaaaag ctcgtacagc aagggatagt tcttcgtgta
    10861 cgtataatcg aaagccaggc ctgcattgtt ggcggcagcc aagcacttgt tgaacgaagt
    10921 ggggtcgggt gtgatcgcgc cgcattcctc ttccatccgc gcgaaaagat tttgcttggc
    10981 ggctagagct tcttctcttc ctatccggcc agcccgggcg gatgcgtaca gcatcttgaa
    11041 ctccgggtag tatcgattca ccacttcggc ctttcgcaga aacagttccg gctccttcga
    11101 gagattctgg tacacaggcg aggccatgcc gaatttctgc tgggcaaact cgattgccgt
    11161 gagaaaaccg atcagggtcg acgccgcctc ggtgacgctg gccggcagct tttcggagtt
    11221 tttatgtacg tgaaagtctt cgtgcggcac gaccaccagc agacgctcca ttgtcgagtt
    11281 ctgaagcgac gtggtcaccg ggcttttgcc gttggctaca gcttcgatcg gatagaagaa
    11341 tacatcgtat ttttcggaat ccacttcgca ggccggttgg ttggtctgct tgagctgcaa
    11401 gtcgtcatag gaagaaggga tttcgagttt gcccgtgtag tagcagcggt agtaagcggc
    11461 aattttgtcc gaggaattca gaaagtttcc gctgcgccgg aagccgagcg ccttttccag
    11521 ctccttcatc tcgcgaatca tctgctcgcg cgccgccggg tcctgcgggg aaggctcgtc
    11581 gccgctgaag caggtttccg ccaaggcgaa agccagcgcg atgatgagaa tgaatcgccg
    11641 tctcattcct ggaaattcct tcttggctcc tcccgctcct attagaccgc cgcgcagcgg
    11701 gtacggttag ccgcagcggc ctgcttcatt atccgccagg gtcgagtcgt ttgctgggcc
    11761 agatcggggg aggtcgcccg ccgccgccaa aaccctcttc ttgcgatgat tcccagcagt
    11821 ggaacgctgc gcttccattt gagcctcttg aagagctgga acatttaagc ctcttgaaga
    11881 gctggaagaa gatagaaagg acaactgcgc gtaggagcca aaggaagatc gccggaatgg
    11941 agtgccggga aactcggccc gaagctttcg atgccctccc agaggcgggc gcgaagtgcc
    12001 cttcagggat tcactgcaaa cagcgggccg cgcgccgttc tctcggcagg cgcggcccgg
    12061 atgcgcgcaa gggattaaag ccgcttcact cggatgttcc ggaactcgat tttcgtgccc
    12121 ggagcatcct cgggatgcgc ccattgtaat ccgattgcgc cgttggccag cttactgtcg
    12181 tgcgcgtcaa cgaccttctg gccattgtaa acaatgacga aatggtcgcc gtcggcggtg
    12241 atgtcgaacg tattccactc gccattgcga atcttggcgt ccgctggcgc cttttgcacg
    12301 ttgaccaggc tagcggtcag ataactgccg cccgtataat ggccggggtc tttgtctcgg
    12361 atttgtactt cataaccctg gccgcccccc ttctttttgc ctttggcttc tcccgggggt
    12421 gcggggtgcg ggcgcgggtt ccgcagcata acgccggagt tgacgttatc cgtggtgcgg
    12481 aactccagcc tcagctcgaa attggcgaag tcctctttcg tcagcagcgt agtcccgcgc
    12541 ggggaatccg tcataatcgt gccgtccacg accttccacg cgccgtttgg attcgtattg
    12601 ggatcccagt tgtcgagagt ctttccgtcg aacagcaaga tccatccttc ggccttttct
    12661 ttcgccgtga gcgtattcgg cgctgcgccg ggcggagcgg ccaataccac cccaactgct
    12721 aaaaccgtga acatcgcgag cattcgcttc ataaggttgc cctccctgga ccgtactatt
    12781 atccataatt ccgtggcggt gtgagagcgc cccgaagaat ttacccggct ccgctgcgcg
    12841 gcgcggaccc taaagcgccc cagtcaagga tggcgcgggg ctcgttagca tggccaggcg
    12901 attgcagcgt ttctctccgg tggaggctat atgcggaaag ttttactctt cgtcctggcg
    12961 gccacatttg gcgtcgctgc ctgggcggac acattaatca tgcgggatgg cgcccggtat
    13021 accggaacgt tgatcgacgg caactcgcgg caagttacgt ttgcggacca aaacggaatg
    13081 cggcggacgt tcaataccag tgaaatacag gccgtagagt tccaatccgg agcctactcg
    13141 caaggccaga ccggcggcgt ccagatgggc gcagcactcc tcccggccgg cacacagatt
    13201 gcggttcgca ccaacgaaaa cattgactca acatcggcgg caacaggccg aacttactcc
    13261 gcgacgatca ctcgtgatgt gcttgaccag aatggcaatg tcgtgattcc gaacggatct
    13321 cctgcgcagc tcatagtgcg aaatatctcc agcggaggca cgttcggctc ggcggagttg
    13381 gctttggatc tgcaatccgt cgatgtcaac ggacagcgtt acacggtaag cacagccgac
    13441 gtgcagcagc agggcgccgg cggccttggc gcaaacaagc gtacgggtga atacgtcggc
    13501 ggcggggcgc tgcttgggac ggtgctgggc gcgattgccg gcggcggcaa gggagcggcg
    13561 atcggagcgc ttggcggcgc ggccgccggg gctggagtgc aggtgctcac aagaggaaaa
    13621 gaagtgcacg tgcccgcaga gacgcagttg accttccggc tcgatcagcc tctccagctc
    13681 cagccgatgc ggcgctaggc gcctgacgtc tccggcccgg cgcccagagg ccgggccgga
    13741 accacacctc taagtcaagc ggcgcacttt gatgttgcga aaggcgatgc tcttgccagg
    13801 cgcgcgctcg ggatgcgccc actgaagacc gatcgctccc gtggtgtgca gagaattgtg
    13861 ggcgtcgagc actttcttcc cgttatacac caccgtgatg tggtcgcctt gcgcggtgat
    13921 ctccatacga ttccattgtc cggggatgat cttggccagg ctggccgtct tggccatgtt
    13981 gaccagactg cccgtcgtgt agccctgctc gtcgatgtcg cggatctgaa tttcatagcc
    14041 gacatcgtgg atgttcccct ggcgcgccat gcgaatgtag accccgctgt tgatatcggg
    14101 cgtcccctga aactcgagct tgaggacgaa gttgtcgtag tcctcgttgc tgtacaacgc
    14161 tccgcccttg ggcgaattcg tcgtaacggc gccatccacg atcttccact cggccgcgcc
    14221 gtgaggcgtc cagccgttta aggttttccc gtcaaataga agtttccagc cttgagcggt
    14281 ttcctggggg gttagggtat tgggcgcttc cggtccaacg gccaacgctg tccagagacc
    14341 gatggttaaa gtcgcagcga gtattcttct catgtcctgt tctcctggga gattttcggg
    14401 ccgcccatgc ttctccggcg caggcgacgc gttgctttgc tgctcagaaa tacgaaaggc
    14461 gcttggacta actcaagcgc cggaccttga tattgcggaa ctcgattttc ttcccggtca
    14521 ccagttccgg atgggcccat tgcaggccga ttttcccgga agcgaacttc gcgtcatgtc
    14581 cgtccagaat ctttttgccg ttgtaaatca cgatgaagtg atcaccctga gcagtgatgt
    14641 caaaacggtt ccactgcccg gggatgatct gcgccttttc agccttgagc acgcccacca
    14701 gactgccggt gtcaaagccg cctggagagt catgcaccgg ccggacatcg ctgtctcgaa
    14761 tattgatctc gtagcccggc atgccttggc ttttgccctt tggcttgggc tggccctttg
    14821 tggcgggatt gtaaggccgc ggcgtatagc gcaaataaat gccgctgttc acatccggcg
    14881 tggtgcgaaa ctcgatttgc agctcaaagt tgtcgaaggt atcgttcgtg aacaggttgc
    14941 cgcccttatc ggaatgcatc ataatcgcgc cgtcctcgac tttccattcg gcgctgttgc
    15001 tggaaggcgt ccagccgttc agcgtatggc cgtcaaacag tagaatccag ccttccgatt
    15061 tttccttcgc gctcaacgta ttcggcggct cgggaggagc ggcgaaagcc gcccatagtc
    15121 cgactgcccc taatacaaat gctatccgct tcataacgcc tcctcctttc gcgcttatta
    15181 tccccaattt tgcgtccggt ttcgagggcg cccgccaagt taagacgcat ggcgctccag
    15241 ccaacccgcc caggaaggga cggtgctgcg ggcgagcgcg aacaggagcc gggccctcgc
    15301 aggtcgctat aataaacatc cacggccggc cgccaatttt agccggaaac aggaccaggg
    15361 tgagtgatga ataaactcgg agttggggtc atcggagtcg gcgagatggg gaagcgccac
    15421 gcggagaacg tgcggtatcg cgttccgaaa gctcgattaa tcgccgtagc ggatgccaat
    15481 ccagagagag cgcggagtgt cgctgcggag ctggaaatcg agcatttcta tgacaacgcc
    15541 gaagatctcg tagcgcgcac cgatatcgac gcggttgtaa tcggctcgcc cccgcgggtc
    15601 cacccggcgc tcatcaggag cgcggctgcg gccaaaaagc acatcctttg tgaaaagccg
    15661 ctcacgctta ccctcgaaga ggcggacgaa gttctggatg cggtatcgaa ggcgggtgtt
    15721 ttttttcagg tggggcacat gcggcgctac gatccaggct atgccgccgc caggcagcga
    15781 atggaagctg gcgaaatcgg cgagccggtg ctgttcaaat cgatcgggcg cgatgcggcc
    15841 ccgccgccgc ccgcctattt tgagtgcggc ctgaacggca cgcttttcct tgattccagc
    15901 gttcacgagt tcgatctggc gcgatggctg atggcggacg aggttgtgga agtccacgct
    15961 ttcgccgccg caaagtcgct gccggaactg gccaagcgcg gcgcttacga ttcaggcgtg
    16021 gtcaacctcc gattcgccgg cggggcgatc ggcaacaccg aatcgttcat gaatgccggc
    16081 tacggctatg atatccgcac ggaaatcgtg ggaaccaaag gcgctctcca aatcgggtat
    16141 ttgggttata cccctctcgt ggttctcaag ccgggcgtga gcagtcacga caacgtccag
    16201 cattggctcc agcgcttcgc ggacgcttat cttcgggaga tgtccgattt tgtggagagc
    16261 gtgctggccg gccgcaggcc acgagtcacg ggatacgacg ggcgccaggc cgtcgccatc
    16321 gctctcgcgg ccgaatgttc gtataaggaa ggccggacgg tccggctgaa gaacccggcc
    16381 accgcctgac ggttcgcagg gcaagccgga gcgaatcgcg gttagacgct gatgaccggg
    16441 caaggggaat cccggataat cgcgtacgcg tcggtgcgaa gccttcccaa tagccctgct
    16501 gcggaagcac ggccgataac caccagcccg gccccgtggt tctcggcggc cgtccggacg
    16561 accttcgata cgcttccgcc tgccagtacc tgctggatct gaatacccgg cggcgcgcac
    16621 tccccgagca tcttgacgac cttctctttc gcctcgctgc tcatcagccg gagcagttct
    16681 gactgaagat acaccgtctc gggcgtgaac tcgaaggagg gaatggcgtg aacgatcacc
    16741 agttcggcat caaattcttt tgcaaaccag ctcgcccacc gcaagtgctc cgaaacgttg
    16801 gaggcaagat cgatggcgca gaggaccgtg cgcactggag gggcggcctc ttcggcgggc
    16861 tcctcgatgt gcactcccgt ccacaccggg cagcgcacgt catgcagcac tttcgcggtc
    16921 accgagccga gaatgaagcg gcgaaaggtg ccccaacccc gggtcggcat catgatcaaa
    16981 tccacatttt ccgagtgtgc gtactcgacg atcttgccgg cgggatcgcc ttccgccagc
    17041 accctgttga cttcgaactc ggccagttcc tcggctagaa aggaatgaag ctgggcgcgc
    17101 acttgcttct ggtgttcggc gagcgtttct tcaactacaa tgcgcttgac ctgcacatcc
    17161 cccggcgaaa gccggaccac ctgcgccacg tgcagcagcg tgattttgga atggaagtgc
    17221 ttggcgagcg gcgcggcgtg ccgcgcggcg acaacggagc ggcgggagaa atcggtcgga
    17281 agcaggatat ggcgcaatga gagcatgagc gtcttctctc ccggatgtta gcagcaaaac
    17341 gcgcgcccgg gagcccgcct cgcggcgcct gtgctgctct ccgccccaac cggggccgcc
    17401 cggcgtttcg atagccacgg tgtgccggac ggagttcggt tgaaataccc gggatggggc
    17461 gccggaaaat tgcctcgccg ccaaaaaacg gccttagcca ttcgagggta taatgcaggt
    17521 ctccttattt tccggcatta ggaagggaaa gctcatgaga tggcgcggcg tggtggccgc
    17581 tttcgcctgc ggggtggcgc tgatgccggc gtgcgggcag cggcagccga agacccggct
    17641 gaagagacag gagtttggaa aaatgcagaa tggcgcgccg gtcgagctgt ttacgctaac
    17701 caataagagc ggcatggaag ctgctgtcac caattacgga ggaattctcg tttctctaaa
    17761 aacacccgac cggaacggcc ggctggctga cgtggttcta ggcttcgatt cgctggatgg
    17821 atatctccag cccgagccct atttcggcgc gatcgtcggc cgctacgcga accgtatcgg
    17881 acacggcacg ttcaccctca acggcgtctc ctacacgctg gcgcgcaaca acggagagaa
    17941 cgcgctgcat ggcggcatca agggttttga caaggtcgtg tggcaagcca aagatgtctc
    18001 caccgacgcg gcccccagcc tggagctgac ctacctgagc aaggacggcg aggaaggcta
    18061 ccccggcaat ctttctgccc gcgtagtcta caccttgacg gacgacaacg agcttaagat
    18121 cgactacacc gcgacgaccg acaaggacac ggtc
//
LOCUS       DASREK010000002         5779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_989571, whole genome shotgun sequence.
ACCESSION   DASREK010000002 DASREK010000000
VERSION     DASREK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989571"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /gene="asnB"
                     /locus_tag="VFA54_00120"
     CDS             <1..1850
                     /gene="asnB"
                     /locus_tag="VFA54_00120"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZS49234.1"
                     /translation="AHRGPDDEGFFLDGRVGLGHRRLSIIDLRGGKQPIFNEDESAVI
                     VFNGEIYNYRELTAQLTAAGHVFSTQSDTETILHAYEQYGDRCVERLRGMFAFAVWDR
                     SKQRLLLARDRLGVKPLYYYAGPRFFAFASEIKSLLEIPEAPREVDPEALDLYLSLRY
                     VPGPRTIFKNIYRLQPGCILVLDRKGMRTEQYWDIEYSEPEPHPEKHFLDGFRELLDQ
                     SVRLRLLSEVPLGVFLSGGLDSSAILAMMTGAAEGSRVKAFSVGYRGLGRKDAQSNEF
                     GFARLAAEAFVAEHHEYHLSAAEFGDFAPELVWRLDEPMADPSAIPLYFLSRLAREHI
                     TVVLSGEGADEILGGYNIYRRMLSLNRLYGTAPSAMSRFAPWAARYTPKGVLRRYVGM
                     SGQPLERRYRGVCRGFTSEWKRRLIGADRGERSEQLLDSIFESRFNAVRYASPLNRML
                     YVDAKIWLPDDLLIKADKMTMANGVELRVPFLDHKLVEFAAAVPDHLKIGPDGGKSLL
                     RKAMQAVLPDVILHRSKKGFPTPVSSWLRSPLRSFARELLLSSGSACSRYMERRVVEE
                     MMHEHERGKCSRHEEIWTLLVFELWHRTFIDRRIEPAVARKEPPLAVAV"
     gene            1869..3068
                     /locus_tag="VFA54_00125"
     CDS             1869..3068
                     /locus_tag="VFA54_00125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49235.1"
                     /translation="MIEGNDIICFSNDWNGDPLSKKHIATRLARKNRILWVNSIGNRN
                     PAVSAHDLRRALKKLLQCCQGLRRVAGNIWVLAPLVIPFHGSRAARRVNRRLLRWRVE
                     QACRTLGFQQPITWTFVPSSADVAGSLGERLLVYHCVDEFSQFTGTNRGAILEMERRL
                     LTRADAVIVSSAKLYESKRAYNPNTFLVRHGVDLEHFRKACLDTTREPEECRRLRRPI
                     IGFFGLIADWVDLQTIRHIALARAQWSFVLIGKVQTDTSPLRGLPNVHLLGRRDYESL
                     PAYCKAFDVAILPFIVNELTVAANPLKLREYLAAGLPVVATPLPEVETLGGRVRVARS
                     PQEFLTQIEDLLRERQGSRLAVSKSMESESWDQKVEELSRVVADCLATPNREAERLRA
                     AGAVPAA"
     gene            3421..5181
                     /locus_tag="VFA54_00130"
     CDS             3421..5181
                     /locus_tag="VFA54_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HZS49236.1"
                     /translation="MSIRPLLILLAMQVVACPAAAQQNAPLTWWTTHSMQKVRPNDPM
                     PFSRRHTAHLYAARNEFEAFQLVFRSDTQNVQGVDVDVSDFRDAGGKDVISKESVTVY
                     LEEFINVQTPSTVEGAVGLWPDPLVPRVDRYAGERRNAFPFTVQQGLNQPVWIEVYVP
                     STAEPGSYSGSVTVSINGEAAFSVPVTLTVWNFTLPSTATLRSSFGFSGVAAVKQHRG
                     RYTTDADLYALTGLYAKAALLHRISIHGGSMAPPRYTFDSSGRIHVDWSAYDAEVAPF
                     LDGSILGARQPLPGARFTSIDVRTPASLQSDEERIAYWTEWTQHFQQNGWLDRLFLYL
                     WDEPAKSDFLQLLQRGHAAAGVSPPLRNLVTTIYTQQLAPVVQIWVPLVNCLEPKPGF
                     DDLCLQTPPLSSYTAEIQKGKQVWFYQSCASHGCSSTGGSYYVGWPSYMIDIFAPANR
                     AMQWIAWKYRMEGELYYSMNQAYSSGTDPWESVYLFGGNGDGTLFYPGRPDRIGGSTD
                     IPIESIRLKLIREGMEDYEYLALLSQLAGPDAADQYAARIVRKPYNWESRPEAFLSVR
                     MEMGRDLNRLAAGPGGPDAR"
     gene            5171..>5779
                     /locus_tag="VFA54_00135"
     CDS             5171..>5779
                     /locus_tag="VFA54_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZS49237.1"
                     /translation="MRDDTPARLIREVRRPLLAAVERLSDSAAEIHSQWRRFLAQLPV
                     SREGLEFLSRLDLTANARRLRAAGAQRLRESAEEYGQELASRGVTAACTAAAVTLYVE
                     ACLPYLLADGQPERLKWAGAVARFASAYQFLLLSGYARQRALERQRLEEKFGRAEQRL
                     QGFSADLGEAYERERRRLARDLHDEIGHDLIVLKLYMEMLALD"
BASE COUNT         1068 a         1730 c         1806 g         1175 t
ORIGIN      
        1 tggcccatcg cggccccgat gacgagggat ttttcctgga cggccgcgtc gggctgggcc
       61 atcgcaggct cagcattatc gatctgcgcg gcggcaagca gccgatcttc aacgaggacg
      121 agtccgccgt aatcgtattc aatggcgaaa tctacaatta tcgcgaactg accgcgcaac
      181 tgaccgccgc cggccacgtc ttcagcacgc agtcggacac agaaaccatc ctgcacgctt
      241 atgagcagta tggcgaccgc tgcgtggagc ggctcagagg aatgttcgct ttcgccgtct
      301 gggatcggtc aaaacagcgc ctcctgctgg cccgagaccg gctgggagtc aagccgctct
      361 attattatgc cggcccacgt ttttttgcct ttgcctccga gatcaagtcg ctgctggaga
      421 ttccggaggc gccgcgggag gtagatccgg aggctttgga cctttatctg tccctgcggt
      481 atgtgcccgg cccgcggacg attttcaaaa acatttaccg cttgcagccc ggctgcattc
      541 tcgtgctcga caggaaggga atgcggacag agcaatactg ggacattgaa tactctgagc
      601 cggagccgca tcctgaaaag catttcctgg acggttttcg ggaactgctg gaccagagcg
      661 tgcggctacg tcttctgtcg gaagtccccc tcggcgtttt cctcagcggc ggcctcgact
      721 cgagcgccat tctggccatg atgacgggag cagcggaggg ctcccgcgtc aaggccttca
      781 gcgtcggcta ccggggattg ggtcgcaagg acgcgcagtc gaacgagttc ggcttcgccc
      841 gcttagcggc cgaggccttc gtcgccgaac atcatgaata ccatttaagc gcagctgagt
      901 tcggcgattt tgcgccggag ctggtgtggc gtctcgatga gccgatggcc gacccgagcg
      961 cgattcctct gtatttcctc tccaggcttg cgcgagaaca cattaccgta gtgctatccg
     1021 gagagggagc ggacgaaatt ctgggcggct acaacatcta ccgccgcatg ctctcgctga
     1081 accgtctcta tggaaccgcg ccgtcggcga tgtcgcgctt cgccccctgg gcggcccgct
     1141 acacgcccaa gggcgttctg cgtagatacg tcggtatgtc cggccaaccc ttggaacggc
     1201 ggtatcgggg cgtgtgccgg ggtttcacca gcgaatggaa acgccgcctg atcggggcgg
     1261 accgcgggga acgctccgag caattgctgg acagcatctt cgaatcacgc ttcaacgctg
     1321 tccgctacgc ttccccgctg aaccgcatgc tctatgtgga cgccaaaatc tggctgcccg
     1381 atgatttgct gatcaaggcc gacaaaatga cgatggccaa cggcgtggag ctgcgggtgc
     1441 cgtttctcga ccacaagctc gtagaattcg cggccgctgt gccggaccac ttgaaaattg
     1501 gccctgatgg cggaaaaagc ttgctgcgaa aagctatgca ggctgtgctg ccggacgtga
     1561 ttctccatcg gtccaaaaaa ggcttcccga ctccggtttc atcctggctt cgttcgcccc
     1621 ttcgcagttt cgcccgcgaa ctgctgctct cctccggctc ggcctgttcc cggtatatgg
     1681 agcgccgcgt cgtcgaagaa atgatgcatg agcacgagcg gggaaagtgc agccggcacg
     1741 aggagatctg gacccttctc gtctttgaac tgtggcatcg cacgtttatc gacaggcgga
     1801 ttgagccggc cgtggcccgc aaggagcctc cgctggcagt ggctgtctga aagacaggga
     1861 ttcggaaaat gatcgagggg aacgacatta tctgcttcag caatgattgg aatggagatc
     1921 cgctcagcaa gaaacacatc gctactcggc tcgcgcgaaa gaaccgcatt ctatgggtga
     1981 actccatcgg caaccggaat ccagccgttt ccgctcacga cctccggcgt gcgctgaaaa
     2041 agcttttaca gtgttgccag ggtctgcggc gcgtcgcagg gaacatttgg gtgcttgccc
     2101 ccctggtgat cccgtttcat gggagccggg cggcccgccg ggtcaaccgg cgactgcttc
     2161 gctggcgcgt cgagcaggcc tgccggacgc tcgggttcca gcagccgatc acatggacct
     2221 tcgtgcccag ctctgcggat gtggccggat ccttgggaga gcggcttctc gtctaccact
     2281 gcgtcgatga gttttcccag tttacgggaa cgaaccgcgg cgcaatcctg gaaatggagc
     2341 gccgtctttt gacaagggcc gatgcggtga tcgtctcttc ggccaagctc tatgagtcca
     2401 agcgcgcgta caaccccaat acatttctcg tgcggcacgg cgttgacctc gagcactttc
     2461 gcaaagcctg tctcgatact acgcgcgagc ccgaagaatg ccggcggctg cgacggccca
     2521 ttataggatt tttcggcctc atagccgact gggtggattt gcaaacgatc cgccatatcg
     2581 ctttggccag agcgcagtgg tcgttcgtgc tgatcggaaa ggtacagacc gacacctcgc
     2641 cgctgcgcgg cctgcccaac gtgcatctgc tcggcaggcg cgattatgaa tctcttcccg
     2701 cttattgcaa ggctttcgat gtcgccattc tgccgtttat cgtcaacgag cttaccgttg
     2761 cggccaatcc tttgaaattg cgcgaatacc tggcggccgg gctgccggtt gtagcgacgc
     2821 cgctgcccga agtggaaacg ctgggcggac gggtgcgcgt tgcccggtct ccacaggagt
     2881 ttttgacgca gatagaggat ctgctgaggg agagacaagg ctcacggctt gctgtttcca
     2941 aaagtatgga aagcgagtcc tgggaccaga aagtggagga actcagccgt gtcgttgcgg
     3001 attgcctggc tacacccaac cgagaagcag agcgcctgcg cgccgccggc gcggttccgg
     3061 cagcgtagcc ggaaaagagt ccgcacgcgc agccgggcgg cccaagagtc gggtcccgcg
     3121 aatacgagaa caaacggcgt ttccggccat cctgccgtgt ttgacctccc ggccgtaagc
     3181 ccagccgcgc ttttcccggg ccgctccggc ccggcgtggg agtggctttg ccggcaaagg
     3241 acgcggcaca tccgccgtct gatatcttct tccagcgccc ggggggagct tgtgcggcgc
     3301 tctgctttgg cgccggtcca ggacgcgggc tatctttgcg atttgctggg cggctgcttt
     3361 ttgcagcctg tccggcggcc ggtgggctgg aggcgctgag cccaggggag gaacaatgct
     3421 atgtctatca ggcctctgtt gattcttctc gcgatgcaag tagtggcttg tcctgcggcc
     3481 gcccagcaaa atgcgccgct aacctggtgg acgacgcact ctatgcaaaa ggtgaggccg
     3541 aacgatccga tgcctttttc ccggcgtcat acggcgcatc tctacgctgc ccgaaatgag
     3601 ttcgaagcct tccagttggt gttccggtcg gatacgcaaa acgtgcaggg cgtggatgtg
     3661 gatgtatcgg attttcgaga cgccggcgga aaggacgtca tttcgaaaga aagcgttacg
     3721 gtctatctgg aggagttcat aaatgttcag acgccgtcca ccgtcgaggg ggcagtgggg
     3781 ctttggccgg atcctctagt cccccgcgtg gaccggtatg cgggcgagcg acgcaacgca
     3841 ttcccgttta ctgtgcagca aggcttgaat caaccggtat ggatcgaagt ttatgttcct
     3901 tccacggccg aacccggctc ctacagcggc tcggtgaccg tttcgataaa cggcgaagca
     3961 gcgttttccg tgccggtaac tctgactgta tggaatttca ctctaccctc gacggccacc
     4021 ttgagatcgt cgtttggctt cagcggggtg gcggctgtga aacagcaccg cggccgctac
     4081 acgaccgacg ccgacctcta cgccctgacc ggtctttatg caaaggccgc tctgctgcac
     4141 cgcatcagca tccacggggg aagcatggcg ccgccccgct atactttcga ctcttcgggc
     4201 cgcattcacg tggactggag cgcctacgac gcggaagtgg cgcccttcct cgacgggtcc
     4261 attttgggag cgcggcaacc gctgcccgga gcgcggttta cctcgatcga cgtgcgcacg
     4321 ccggccagct tgcaaagcga cgaagaaaga atcgcgtatt ggaccgagtg gacgcagcac
     4381 tttcaacaga atggttggct cgatcgtctc tttttgtatt tgtgggacga gccagccaag
     4441 agcgattttc tacaactgct ccagcggggc cacgccgcgg cgggcgtctc gccgccgctc
     4501 cgcaacttgg tcactacgat ctacacgcag caactggcgc cggtggtgca aatctgggtt
     4561 ccgctcgtga attgcctcga acccaagccc ggttttgacg atctctgcct ccagacgccg
     4621 cccttgagct cgtataccgc ggagattcag aagggcaagc aggtttggtt ttaccaatcc
     4681 tgcgcgagtc acggatgcag ttcgacgggc gggtcttatt acgtgggctg gcccagctac
     4741 atgatcgaca tttttgcgcc cgcaaaccgc gcgatgcagt ggatcgcctg gaaataccgc
     4801 atggaaggtg aactgtacta cagcatgaat caggcgtata gttccgggac cgatccctgg
     4861 gaaagcgtct atttgttcgg aggcaacggc gacggcacat tgttttaccc tggacgtccg
     4921 gacaggattg gaggctccac ggacattccg atcgagagca ttcgcctcaa gctgattcgc
     4981 gagggtatgg aggactacga gtatctggcg ctgctctccc agttggcggg ccccgatgca
     5041 gcggaccagt atgcggcccg gatcgtgagg aaaccgtaca attgggagtc gcgtccagaa
     5101 gcatttctga gcgtgcggat ggaaatgggc cgggatctga accgattggc agccggacct
     5161 ggcgggccag atgcgcgatg acacgccggc ccgcttaatc cgcgaggtcc ggcggccctt
     5221 gctggccgcc gtggagcggc tttccgactc agccgccgaa atacattccc agtggcgccg
     5281 cttcctggct cagcttcctg tgagccgcga gggactcgaa tttctctcgc ggctggatct
     5341 cacagccaat gcgcggcggc tgcgcgcggc tggggcgcag cgcttgcgcg agagtgcgga
     5401 agagtacggg caggagcttg ccagccgcgg tgtgaccgca gcctgcacgg ccgccgccgt
     5461 gacgctatac gtggaggcgt gcctgcccta cctgctggcg gatggccagc ccgagcggct
     5521 gaaatgggcg ggagccgtag caaggtttgc ttcggcttat cagtttctcc tgctctccgg
     5581 ctacgcccgg cagcgcgcgc tcgagcgcca gcggctcgag gagaaattcg gacgcgccga
     5641 gcagcgcctg caaggctttt ccgccgacct gggggaggcc tacgagaggg agcgccggcg
     5701 cctggctcgc gacctgcacg acgagatcgg ccacgatctg atcgtgctaa agctttacat
     5761 ggagatgctc gcgctggat
//
LOCUS       DASREK010000003         5986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_989830, whole genome shotgun sequence.
ACCESSION   DASREK010000003 DASREK010000000
VERSION     DASREK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989830"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFA54_00140"
     CDS             complement(<1..415)
                     /locus_tag="VFA54_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49238.1"
                     /translation="MRSIFGLPFLLAALPALLPGAAPQPGPAGRASLNTNDNRALRIS
                     LKAAERAIDDRIQQLMARAPFALLGTTRGVYLFGYGAVFTLEVNLVPVAGLSPFRPAY
                     TSQEIRNLNRQKREKLSALKTGLRQLLMEEAPALPQ"
     gene            complement(421..942)
                     /locus_tag="VFA54_00145"
     CDS             complement(421..942)
                     /locus_tag="VFA54_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZS49239.1"
                     /translation="MSCSPFDLRDFWLEELGAADRRQVEKHLEACARCREELERLRMT
                     QRALLALKDEEVPRRIAFISDKVFEPSGMSRWWAAFLLRVPRFALSASLLLAVLFGGL
                     WVSKPSITLERERWQIAFGGRDSQSERAVAAMQARYEADMRDVKENYELLVKELNVLY
                     MQSAQARPAAFRQ"
     gene            complement(977..1549)
                     /locus_tag="VFA54_00150"
     CDS             complement(977..1549)
                     /locus_tag="VFA54_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS49240.1"
                     /translation="MIFREVEDRDLVLAARKGDVEAYNQLVSRWEKRVYNYLLRVVKD
                     REDALDLTQETFLKAYRYLGKLDDADCFPRWLFRIAHNEAFSQFRREKPESQELSECG
                     GGRAIPIAGSAVYPLELELAVGSALEQLSAEQREAVVLKIYQGFKFDEIAQIQSCPVS
                     TVKSRLYAAFDLLKVLLTTGNALTTEKSVS"
     gene            1794..2303
                     /locus_tag="VFA54_00155"
     CDS             1794..2303
                     /locus_tag="VFA54_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZS49241.1"
                     /translation="MPISEALLPEFDHEMANTRKTLERVPWEKPDWKPHAKSMTIQRL
                     ASHVAEIPGWVGVTITCDSFDVAPPGGQPVQPTSATSRTELLEIFDRNVSAARAAIAG
                     ASDAQLMKPWSLLNGGKPVFTMPRAAVLRSFIMNHSIHHRAQLGVYLRLNDVPVPSTY
                     GPSADEGGM"
     gene            2418..3227
                     /locus_tag="VFA54_00160"
     CDS             2418..3227
                     /locus_tag="VFA54_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS49242.1"
                     /translation="MTHIPGTYFLRNLVLHRSLVFHLVRRDFQQRFVGSVVGWVWGII
                     HPLVLLVAYTFIFTYCLRMRLGPGQATDNYPIFLFAGMLPWLLFQETVQRSASALVEY
                     ANLIKKSVFPAEIVPVAIFLSNLLSHFLALLLLVTAVAVWLKHISAMLLVLPVYFFLI
                     GLFSLGLAWIASSLNVFLRDTAQVLSVLLTFWFWLTPIFIAEAQFPRAVHWLVTFNPL
                     AYAVRGYRTSILSSHLPAARDLLLLTLYAVLSFVLGGLFFRHTKKGFADVL"
     gene            complement(3250..5469)
                     /locus_tag="VFA54_00165"
     CDS             complement(3250..5469)
                     /locus_tag="VFA54_00165"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HZS49243.1"
                     /translation="MHHIVCSRNVNRQQQRLEAGASTGIGTGLSRFTPHAILLAEALV
                     FHRRVLFRSGYVIPWDFRYFHLSLASILAEAVKRGQLPLWDPLTYCGRPVYANFQIQA
                     FYPPSILAVLIHNLLGRHQLIHVLQWQLVLHVFLAGVFTYHLLRKLGVGRPAALAGAA
                     VFELGGVLAAQVEHLGFVDAAAWIPGAWIAVLVLAESFSWRKLAALAASLAMTILAGY
                     PAMAAAAFGSCLLLGLALIAFRLAQRRLFGLIAASIGLSLGFAAIQLLPGAELRRLSI
                     AKYRSDYMGKGEYALGAGLPPAALVSLISPNHYGIFDLTRYSGKWEPTVLYLYCGITG
                     LLLATVAASMRRDRLTRLFLVMTLASALWMLGRFTPIGRALLALAPGAVRTVAYPELA
                     AAPFVLSMAVLAGLGADWVSQKAERAAAAYAIALLVAADLILVGSGRPMNTASLREEP
                     GITTYSFDGSSALLSQVRKLANAAVPPLRIDTIDDSALWAMGSPLTLVPAAGGDEPMA
                     PARVVQARLAYCDGERWRAYCQVNNPDSPVIDLLNIGYLLSRTPMPEAALAQGRFVKT
                     AGIPGRLAYRNTRVLPRFFLVHAIRTAANEDDAAAILRSKTFDLRSEAVVEGAVSLLR
                     GSGAPAGAVSIVDYGMRRVVLEAESASEAWLVSSETHYPGWRAWVDGREEPIYYTNLA
                     FRGLHLPAGRHTIVFYFAPRILWWGAAVSGVFGIALLLMSASGTRPPLAFTRRRGSR"
     gene            5530..>5986
                     /locus_tag="VFA54_00170"
     CDS             5530..>5986
                     /locus_tag="VFA54_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS49244.1"
                     /translation="MISFRAVSKSYPIYDSPGDRLRELLLFNRRSFHKDFWALRDVSF
                     DVERGATFCLIGENGSGKSTTLQLIAGVFPPSRGEVKIGGRVSALLELGSGFNPEFSG
                     RDNVFLNGAILGLSTAEVARRLDDIVRFASIGDFFDQPVRTYSSGMVVRL"
BASE COUNT         1220 a         1883 c         1779 g         1104 t
ORIGIN      
        1 cttggggaag cgccggcgct tcttccatca gcagctgccg cagacctgtt ttgagcgcgc
       61 tcagcttttc ccgtttctgc cggttcaggt ttcgaatctc ctgtgacgtg taagcgggac
      121 gaaaggggct taagccggcc acgggcacca gattcacctc cagggtgaac acggcgccgt
      181 acccaaagag gtagacgccg cgggtggttc ccagcagggc gaaaggcgcc cgggccatca
      241 actgctggat ccggtcatca atggctcgct cggccgcttt cagagaaatg cgcagcgccc
      301 gattgtcgtt ggtgttcaac gaggcccggc cggcgggtcc gggctggggg gcggccccgg
      361 gcaaaagcgc gggaagcgcc gcaagcagaa aaggcagacc gaagatggat cgcatacttt
      421 tcattgccgg aacgccgcgg gccgggcctg cgccgactgc atgtaaagaa cgttcagctc
      481 tttgaccagc aactcgtaat tttccttcac gtcgcgcatg tcggcttcat agcgcgcctg
      541 catggctgct acggctcgct cgctttgcga gtcgcggccg ccaaaagcga tctgccagcg
      601 ctctcgctcc aaggtaatgc tgggcttcga aacccacagc ccgccgaaga gcacggccag
      661 cagcagcgac gcggacaagg cgaaccgcgg cacacgcagc aagaatgccg cccaccaccg
      721 cgacatgccg gacggctcga agaccttgtc cgagataaac gcgatgcgcc gcggaacctc
      781 ctcgtccttc agcgccagca gggcgcgctg cgtcatacgc agccgttcca gttcctcccg
      841 gcagcgggcg cacgcttcca aatgcttctc cacttgccgc ctgtctgcgg cgccaagctc
      901 ttccaaccaa aagtcgcgca aatcaaatgg cgagcagctc atagaaaacc ttccttttcc
      961 accgcagaga atgcgctcaa gaaacgcttt tttcagtggt gagagcgttc cccgtggtga
     1021 gcaatacttt caggaggtca aaggccgcat agaggcggga cttcacggtg gaaaccgggc
     1081 acgactgaat ttgggcgatc tcatcgaact tgaagccctg atagatcttc agcacgacgg
     1141 cctcccgctg ctcggcgctc agttgttcca gggcgctccc caccgccagc tccagctcga
     1201 gcggatagac cgccgagccg gcaatcggta tggctcgccc gccgccgcac tcagacagct
     1261 cctgggattc cggcttctct ctccggaatt gcgagaaggc ttcgttatgc gcgatgcgga
     1321 agagccaccg cgggaaacag tccgcgtcat ccagcttccc cagatagcga tacgctttca
     1381 aaaacgtctc ctgggtgagg tcgagagcgt cttcccggtc tttcaccacg cgcagcaggt
     1441 agttataaac acgcttctcc caccgggaaa cgagttggtt atacgcctcc acgtcccctt
     1501 tgcgcgcggc caacaccaag tcccggtctt cgacctcgcg gaaaatcaac tttgactcct
     1561 gatgagagat gacgcggccc agcggtaaaa agtctgggaa tccacgaatt tcgccccact
     1621 ataacacgaa ccggagcaca tgcaggcgtc tccaaggcgg ctggtggaag ccccctccgg
     1681 gcaacccagg gccatccggc cgcattcccg gggtttggcc gcactgtttg acaaacaggc
     1741 cagaccgtaa cttcttcgcc gctagagtag aattccgttg aatggagaag cctatgccaa
     1801 ttagcgaagc attgctgccc gaattcgatc acgaaatggc caatacgcgc aagaccttag
     1861 agagagttcc gtgggagaag ccggactgga aaccccacgc aaagtccatg accatccaac
     1921 ggctggccag ccatgtagct gaaatcccgg gctgggtcgg cgtaacgatc acctgcgact
     1981 cgttcgacgt cgctccgccg ggcggccagc cggttcagcc gacttcggcc acttcaagaa
     2041 cggagttgct ggagattttt gacaggaacg tctcggcggc tcgcgcggcg atcgctggcg
     2101 ccagcgacgc ccagttgatg aagccgtgga gtctgttgaa cggcggaaaa ccggtcttca
     2161 ccatgccgcg ggccgccgtg cttcgcagtt ttatcatgaa ccacagcatt catcaccgcg
     2221 cccaactcgg ggtctacctg cggctgaacg atgtcccggt accgtcgact tacggtccct
     2281 ccgcggacga aggcgggatg tagtagcgcg atccggcaga atcaaacccc tgccactttt
     2341 ccggaagagc ccagccggct cgggcggcgc ctggccgccg cccggcagcc tgcaagctat
     2401 actggccgtt aaaggccatg acgcacatac cggggaccta tttcctgcga aatctggtgc
     2461 tccaccgcag tctggttttt catctcgtcc gccgggattt tcaacagcgc ttcgtaggct
     2521 cggttgtggg ctgggtttgg ggcatcatac atccgctggt tttgttagta gcctacacgt
     2581 tcatcttcac ctactgccta cgaatgcgcc ttggcccagg ccaggcgacc gacaattacc
     2641 cgatttttct tttcgccggc atgcttccat ggctgctgtt ccaggaaacg gtccagcggt
     2701 cggcttcagc gctggtcgag tacgccaacc tcatcaaaaa aagcgtgttt cctgccgaga
     2761 tcgtgccggt agccattttt ctgtcgaacc tgctgagcca cttcctggcc ctgctgctgt
     2821 tggtgacggc ggtggcggtg tggctcaagc acataagcgc tatgctgctc gtgctgccgg
     2881 tgtatttttt cctcatcggc ttgttcagtc tgggcctggc ctggatcgcc tcgagtctga
     2941 atgtctttct gcgcgacacc gcccaagtgc tctcggttct tctcactttc tggttctggc
     3001 tgacgcccat tttcatcgcg gaagcgcagt ttccccgcgc cgtccactgg ctggtgacct
     3061 tcaacccact ggcctacgcg gtgcgtggct atcgcacgag catcctctcc tcgcatctgc
     3121 ccgcggctcg cgatttgctg ctgctaacgc tttacgcggt gttgtctttt gtcctgggcg
     3181 gcctgttttt ccggcacacg aaaaaaggat ttgccgacgt gctatagcgc tcggcatccg
     3241 aactgcatgc taccgagaac ctctacggcg agtgaaggcg aggggcggcc gagtcccgct
     3301 ggcggacatc aacaacagag cgattccaaa caccccgctg acggcggctc cccaccacaa
     3361 aatgcgggga gcaaaataga agacgatcgt gtggcggccc gccggcaagt gcagccctcg
     3421 aaaagccagg ttggtgtaat aaatcggttc ctcgcggcca tccacccagg cgcgccatcc
     3481 ggggtagtgt gtttccgagc tgaccagcca ggcttctgag gccgattcgg cttcgagcac
     3541 aacgcggcgc attccatagt cgacgatgct gacggcgccg gcgggtgcgc ccgagcctcg
     3601 taacaggctg accgcgccct cgacaaccgc ctcgcttcgc aggtcgaaag ttttagaacg
     3661 caatatcgcc gcggcgtcat cttcgttcgc ggccgtccgg attgcatgca ctaagaaaaa
     3721 ccggggcagc accctggtat ttctgtaagc cagccggcca ggtatgccgg cggtctttac
     3781 aaaccgtccc tgcgcaagag ccgcttcggg catcggtgtc cgtgaaagta gatacccgat
     3841 attcagcagg tcgatcaccg gcgaatccgg gttgttcacc tgacaatacg ctctccatcg
     3901 ttcgccgtca cagtaagcca gtctggcctg aaccaccctt gcaggggcca ttggctcgtc
     3961 tccgccggct gcgggaacca gcgtaagcgg cgagcccatc gcccaaaggg ctgaatcgtc
     4021 gatcgtatcg atgcgtagag gcgggaccgc cgcgttggcc agcttgcgta cctgagacag
     4081 cagggcgctg ctgccatcga agctgtaagt agtaatccct ggctcttcgc ggagagaagc
     4141 cgtattcatt ggacgtcccg atcctaccag gatcaggtcg gccgccacca gcagcgcgat
     4201 tgcataggcg gccgcggctc gctcggcctt ttgagagacc cagtcggccc ccaagcccgc
     4261 caggaccgcc atactcaaca cgaagggcgc cgccgcaagt tccggatagg cgacggtccg
     4321 aacggcgccg ggcgccaggg cgaggagggc ccttccgatc ggagtgaacc gccccagcat
     4381 ccagagagca gaggccagcg tcataaccag gaatagtcgc gtgagccgat cgcgccgcat
     4441 ggaggcggcg accgtcgcca gcagcaaccc ggtgatcccg caatacaggt acagcaccgt
     4501 gggctcccat ttgccggaat agcgggtgag atcgaagatg ccataatgat tgggcgagat
     4561 cagagaaacc aaggcggcgg gcggcaagcc ggcgccgagg gcatactcgc ctttccccat
     4621 gtaatccgag cggtatttcg cgatgctcag gcgccgcagt tcggcgccgg gaagaagctg
     4681 aatggctgcg aagcccagcg aaaggccgat agacgcagca atcaggccaa aaagccgccg
     4741 ctgcgccaga cggaaagcga tgagggccag gcccagaaga aggcaagaac caaaagccgc
     4801 cgccgccatc gccggatagc ctgccaggat tgtcatagcc aggcttgcgg ccaaggcggc
     4861 cagcttccgc caggaaaagc tttctgccag gaccagaacc gcgatccagg cgcccgggat
     4921 ccaagctgcg gcgtccacaa agcccaaatg ctccacctgc gccgccaaaa ctccgcccag
     4981 ctcgaatacg gccgcgccgg ccagagccgc cggacggccc accccgagct tccggagaag
     5041 atgataggtg aacacgccgg cgagaaaaac gtgcagcaca agctgccact gcaaaacatg
     5101 gatgagctga tgcctcccga gcaagttgtg aatcagaacg gccaaaatgg acggaggata
     5161 aaacgcctga atctgaaaat tcgcgtatac gggccggccg caataggtaa gtggatccca
     5221 caacggcaac tggccgcgct tgaccgcctc agccaggatc gaagcgagcg aaagatgaaa
     5281 gtaacggaaa tcccagggaa tgacgtagcc gctgcggaac agcacccgcc ggtgaaagac
     5341 gagagcctcg gccagcaata tggcatgcgg cgtgaaacga gagaggcccg ttccgattcc
     5401 cgtggaggcg cccgcttcca gccgctgctg ctgccggttc acgttcctgg agcatacgat
     5461 atggtgcaac aggacacaag cggccttgag gagccggcaa tcggctccgg gtgttctaga
     5521 ctaattccga tgatttcttt tcgggcggtt tcgaaaagct accccatcta cgattcgccc
     5581 ggagaccgcc tgcgcgagct cctgctgttc aaccggcgct cgtttcacaa agacttctgg
     5641 gcgctccggg atgtaagctt cgacgtggag cggggggcga cgttttgcct gattggcgag
     5701 aacggctcgg ggaaaagcac gaccctgcaa ttgatagcgg gcgtttttcc gcccagccgg
     5761 ggcgaagtaa aaattggggg gcgcgtttcg gcgctgctgg agctgggttc cggcttcaac
     5821 ccggagttca gcggccgcga caatgtcttc ctgaacggag ccattctcgg gctttccacc
     5881 gcggaggtcg cgcggcgtct ggacgacatc gtccgcttcg cgtccatcgg cgacttcttc
     5941 gatcagccgg tgcgcacata ttcgagcggc atggtggtgc ggctcg
//
LOCUS       DASREK010000004        21757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1814079, whole genome shotgun sequence.
ACCESSION   DASREK010000004 DASREK010000000
VERSION     DASREK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 21757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21757
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814079"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFA54_00175"
     CDS             <1..923
                     /locus_tag="VFA54_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZS49245.1"
                     /translation="MDLLIYLPVAARTPTPLLLNLSFSANSSVVDDPGVKPGEIWSRE
                     KKKIPASSGRSFGKLNIMPFLEQGIGVATVYYGDIEPDFPEGIAYGVRKLYFQPGQSE
                     PAPDEWGAIAAWAWGLSRAMDYLETDKAVDAKRVAILGVSRLGKTVLWAGARDPRFAA
                     VIASCSGEGGAALSRRNYGETIKHLNARFGYQFCGNYQKYGDHVDQFPVDAHMLLALI
                     APRPLFLQTGDKDLWSDPRGEFLAAVAASPVYRLLGKQGLGTDHMPPAGQAILHTLGY
                     EMHSGGHGALPSDWKLFLKFLQIQLLARKG"
     gene            complement(1259..1816)
                     /locus_tag="VFA54_00180"
     CDS             complement(1259..1816)
                     /locus_tag="VFA54_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HZS49246.1"
                     /translation="MSGQEERLPYPATAAARSNLGIARLPVSILLDQVRSLYNVGAFF
                     RIADAAGLSRLYLIGITGHPPNRALAKTALGAQETVSWEHHWDLAPALQRIREEGCEM
                     AAIETSVRSVDLFEWQPRFPLCVVFGHEIEGIRRELSEQCDVHVCIPMLGRKHSLNVA
                     VAGGIVAYELLRKYRELTKAALRAG"
     gene            complement(1813..2001)
                     /locus_tag="VFA54_00185"
     CDS             complement(1813..2001)
                     /locus_tag="VFA54_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49247.1"
                     /translation="MNHTTPIHTFWSIIGMTIAALVVTRCLEISGQRAMPIFVVFMLL
                     GVPLGEFLERRSKRSKAR"
     gene            complement(2026..2469)
                     /gene="dut"
                     /locus_tag="VFA54_00190"
     CDS             complement(2026..2469)
                     /gene="dut"
                     /locus_tag="VFA54_00190"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZS49248.1"
                     /translation="MTPVTISVRLVHPRAQLPRFAHSSQEEAGMDLYAAEEARLKPGE
                     WAVVPTGLILEIPPGYEGQVRPRSGLALRHGVTLLNSPGTIDPGYRGEVRILMVNHGR
                     QEFHIRPGDRIAQLVIASYAAVEWKIEPALTPTARGAGGFGSTGV"
     gene            2532..3590
                     /locus_tag="VFA54_00195"
     CDS             2532..3590
                     /locus_tag="VFA54_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZS49249.1"
                     /translation="MISIRSYLEKLAGEEGLRSAFLKLIQSLVRVMQEKMIKADAEEH
                     NWLCANLDSLQRRLMTDTSPKNIEVVAALLTKTLEDHWLKLGIQFDNRQHEFKRIINL
                     LTESAARLDGQNKHFYLSLREAVQNFQDISQMEDITYVRRKLTEQVANLEETVQRQEG
                     VSNETVGRLQSELEQAHRQIASLIEAVTTDSLTRLPARQAAEKALREHIENNEPFVLG
                     MAVVERLELINLRYGVDSGDEVVRKFARQLRENMPERTLLYRWGGPAFLSLVPRMGAG
                     ELRVLLQKVLSEIAAEPIELEAKGAGFIRITSRFAVHEWTPGQGAEKMVRLVDIFCFS
                     PKGESGDADSRHISKAAI"
     gene            3623..5083
                     /locus_tag="VFA54_00200"
     CDS             3623..5083
                     /locus_tag="VFA54_00200"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZS49250.1"
                     /translation="MSSTGANRVIAVSPMPPEKSAYALARYSRSPDSISQSLDWVRAH
                     DSAKFLESYYFQYGHASIADLGHTIFCFEGISELAAVEIEDEPLWDGQAKSSRYQDFS
                     RSGFVVPPEIAETGGAAVYKDTAGCLLEAYLGIHQRAFDYLVERTPKPESMKAEAYRR
                     ALAARAFDVARYVLFLGIPTNVGQVTSIRTLEKQIRRLSVSRYAEIRQIAAEMRDACA
                     QPPVCVWDAASSREPLAPTLARHADPDVYLRHARRNVAEWAAQNLPAGAFSTVAAESS
                     VDLVRPRDAVEEIVATLLYSVTQFPFRAIYDQVSGWSASRKREVIDAAVRDRSGREEL
                     LREFRSGYAFVFDIVTDIGAYRDLHRHRRCQQIRQDFTAELGLETPALIAEAGAQNIF
                     HHALHEARLAREQLAAVSPEATHYLLPFAARVRFLYKMDFAEAEYVSRLRSGVKGHFA
                     YRAIAWKMKEKVMEVEPDLGRLIEATPPWVEDPLIR"
     gene            complement(5149..6180)
                     /gene="aroF"
                     /locus_tag="VFA54_00205"
     CDS             complement(5149..6180)
                     /gene="aroF"
                     /locus_tag="VFA54_00205"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZS49251.1"
                     /translation="MVISMKPGATKDQIEHVCRRIEEFGYRVHSIRGDERVVIAAVGI
                     GDVTHCLESLEAAPGVESAVRISAPYKFVSREYKKERTQIKVDGAEIGGEEFIVIAGP
                     CSVENEKQILTTAAGVARAGAKLLRGGAFKPRTSPYDFQGLELEGLKLLRRAREETGL
                     SIITEVMSDGDVGLVAEYADVLQIGARNMQNYALLKTLGKAGRPVMLKRGLSSTIKEL
                     LLAAEYVTSHGNPDVILCERGIRTFETATRNTFDIGAVPVLRELTHLPVVLDPAHATG
                     KSSLIPPLARASVAVGADGLMVEVHPCPEEAFSDGAQSLTIEQFERMMQDLGPYLKIW
                     KEERAAVAV"
     gene            complement(6449..7597)
                     /gene="nagA"
                     /locus_tag="VFA54_00210"
     CDS             complement(6449..7597)
                     /gene="nagA"
                     /locus_tag="VFA54_00210"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HZS49252.1"
                     /translation="MNKTSVREHVLGISADSGRPVEIETWGGRITAVQESEADPAGVW
                     VAPGLIDLQVNGFAGVDYNSLNTSLEEIGRSIEAIHSTGVTRFLPTVITGPRERMCGA
                     LRNLARAKREIPQGASIAGIHVEGPFISPDDGPRGAHPREHVRPADREEFLAMQEAAE
                     GNIRLLTLAPETPGATELIEFLVDQGVVVSLGHTGASPEQIREAIRAGATMSTHLGNG
                     AHPILPRHPNYIWEQMAADELYASFIVDGIHLPQAFLKCAIRAKGVTRSVLVTDASSP
                     AGCSPGPYRLGEVLVELTPEGRVQIAGSQRLAGSALRLDRAVANVMRFTGISLNQAWR
                     MASINAARAVRLEGRMGFLEPGDMADFVLFRFDAVRFSVVVDKTIVAA"
     gene            8132..8914
                     /locus_tag="VFA54_00215"
     CDS             8132..8914
                     /locus_tag="VFA54_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZS49253.1"
                     /translation="MLATLYGIPIGYDVTGQGIPLMFLHAFPLDRSMFRPLPGARLIT
                     FDAPGAGESGLPPGDLSMEAVADIAAGLLDLLGIEKAVVGGISMGAYAAFRFVCRHPR
                     RLLGLILSDTRAAADSAEGRKARYEMMEAARSLGSAEIARRMLPRLLGETTRRENPAL
                     VEQVRLMIGKQDPEGIARLLEALAERTDSTALLKQITVPTLVVAGEEDVIATVAESSE
                     MAKRIPGARLAIIPKAGHLPNLEQPERFEKAVESFLQEIGRE"
     gene            complement(8989..9930)
                     /locus_tag="VFA54_00220"
     CDS             complement(8989..9930)
                     /locus_tag="VFA54_00220"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZS49254.1"
                     /translation="MIKPTEKIWHNGEFINWDDARVHILSHTLHYGSSVFEGIRCYST
                     PAGPAIFRAPEHIRRLADSARVYRMEIGFTPAQLIEAMVELVRVNRMDSCYIRPIAFR
                     GYGEMGVLPFHNPIEVYIACWEWGKYLGPEALAEGVDVCVSSWTRLAPNTLPAMAKAS
                     ANYMNSQLIKMEAITNGYAEGIALDVAGYVSEGSGENIFVVRDEHLYTPPLGTSALPG
                     ITRDTVLRLARDLNIEVIEQAIPREMLYIADEIFFTGTAAEITPIRSVDRIQIGSGRR
                     GPITERLQKRFFSVIQGNDPYGWLTNVNARKPVAIVG"
     gene            10088..11437
                     /locus_tag="VFA54_00225"
     CDS             10088..11437
                     /locus_tag="VFA54_00225"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZS49255.1"
                     /translation="MRKFHVFGCLGVLWLAVSAAAAPREIKPGFNLFSRDQDIQLGRE
                     AQQQVEKEVPVVHNAPELENYISQLGMKLAKVSQAPDYPYNFHIVADKNINAFALPGG
                     PVYVHTATIAAADNEAQLAGVLAHEISHVALRHSTNQASKAYAWQIPLAIAGGAAGGS
                     LLGQLAQLGIGLGVNSVLLKYSRDAEHDADIVGAQTMARAGYDPVEMARFFEKLKSQG
                     GGGSALQFLSDHPDPGNRVQYVEAEVKTFPPRQYTKGSPDFARYRQMAAQIPAPQLKR
                     SYNDQPSGNHPHASFPSSDLRTFRGAGFHLSYPSNWQPFGNENEAAITIAPRDGLVQG
                     SEGVQIGLGMIAGYFSPDSNNLTTATNQFVEDLQAKNAGLRPLRGQRRSTTVDGVNAE
                     TLLLSGASPLGRQREIDSLLTALRPQGLFYLVLIAPESDYNSLRPIFVQIENSVRFQ"
     gene            11440..12096
                     /locus_tag="VFA54_00230"
     CDS             11440..12096
                     /locus_tag="VFA54_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZS49256.1"
                     /translation="MIQAIFFDFDGVLVDSEPLHFACWAEALAQHGIAITAESYYRRF
                     TGVSDHSMIDALCREFGAEGDRPLFTACFHRKKQLYRERAAEFCHIPPELVTFLADLS
                     AAYRLGVVSSSDRPDVEPHLAAHGVRRWFRAVVCREDVRNLKPAPDPYLRALELLNDG
                     DGAAVKSAECLVVEDSAPGEEAGRQAGMRVLRVRRPEEVADVLRRELDGTSDKIYYVK
                     "
     gene            12190..13452
                     /locus_tag="VFA54_00235"
     CDS             12190..13452
                     /locus_tag="VFA54_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS49257.1"
                     /translation="MTILPKWRPWLRLGRIDLMWLLVMAALAVLASTRPRHSPYEFLA
                     LGAIALMQVAEMRFPVFASRGGTIVAVVIKLALCWVLVGVTEGIESSYYLIFILPVVS
                     AASTFGLAGTLLTAFAGSLAYLSFLGFVWDRYELPPEGARELGIRILFIFMTGILLNH
                     FVTENRRQAELYRKLADDLAQANRDLHQAQAQVRRSERLAALGQLSAGLAHELRNPLG
                     VIRGSAELVSKNVSAENEVVREMAALIISEADRTNGLITRFLEFARPSPLHRCQADLH
                     AVIQEAIHQVQRSLPSGANGLRLETDFCKDMQPFSFDPELMERVFFNLLENGVQAMPG
                     GGELKITTRRLGDVVQTEVSDTGAGIAPENLESIFNPFFTTKQNGVGLGLAIVSKIVD
                     DHNGKITVSSQPGRGTTFTVILPLSEAS"
     gene            13455..14813
                     /locus_tag="VFA54_00240"
     CDS             13455..14813
                     /locus_tag="VFA54_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS49258.1"
                     /translation="MKKRLLVVEDEANMLRVLELQLAGAGFAVEKASNAEQALAILER
                     GGLDLVLTDLRLPGMDGLQLLERIRGIDASLPVIIMTAFGTVETAVQAMKAGASDYIL
                     KPFSLDDLMMTIDKVLELHTLRDENRRLKDELGHKYQFDNIIGKSPKMQEVFAAVSRV
                     APTRATVLLAGESGIGKDLIARAIHHHSPRRDRPFVKINCSALPENLMESELFGYEKG
                     AFTGAASARAGKFEQADGGTAFLDEIGDVPPPVQVKLLRVLQEREFERLGSNKTLRVD
                     VRVIAATNQDLRAALEQGAFREDLYYRLNVVPINIPPLRERKEDIPYLVEHFLGKYSE
                     ETGGRVRSITPAAMEKLIAYHWPGNVRELENIIQRAMVLAPGPALEPGDIQLDSERRA
                     AATADGVPFLPEGMTLDQYEQQLIREALRRCDGNKSQAARLLGLTRNALRYRLTQMGI
                     EA"
     gene            complement(14949..15194)
                     /locus_tag="VFA54_00245"
     CDS             complement(14949..15194)
                     /locus_tag="VFA54_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49259.1"
                     /translation="MGFPFWSSELLLIAPALKLNNLLSKDGSFDLRPMGSIHAALFLL
                     AAIWLAPLLEAVSPGFHNSKNVARALENLHLDESTRK"
     gene            15739..17940
                     /locus_tag="VFA54_00250"
     CDS             15739..17940
                     /locus_tag="VFA54_00250"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HZS49260.1"
                     /translation="MPTRKHKAIVIGCALAIAAAPFLTAQSPSYPSSGYPSGQQIAGP
                     AVARVSLLNGEVSTQRGDSGDWIAAAVNAPLVAGDRVYAAAGSRAEIQFDSANFLRIG
                     QETEIQLADLEPGQHQVQISKGIITYGILRNSSARVEIDTPLVAIRPTERGSYRVEVS
                     ENGQTQITVRGGRAEVSSRQGTEAIEGGHTLIVRADDPNSDVEVRDMPAPYPDQWDRW
                     NDSRDAQLTRTASYQYVNPEVYGVEDLDAYGAWTQVPGYGPVWYPRVAATWAPYSYGR
                     WCWIDGYGWTWVDYDPWGWAPFHYGRWFWRAGFGWGWWPGPVTVVSPWAPALVGFFGF
                     GAASGFSVGFGFSNIGWVPLAPFEPLHPWWGHGFGSRTVITNINIVNVTNIRNVYRNA
                     AVNGGVRAIAVQEFNQGRTFNHMAVNPAMISNAAQIRGMLPVVPSRNALQVSTHPVNR
                     AAVPQSGAAQNRFFAVHQAPAVRSSFVQQQQQIAQALNGGRIQNRPAMRGNTGFSSGL
                     PASPANTLRSPQGNNLVQGQSQFQAAPAAQVRGGATGKASGGWRSLGPAAWPSQSGLA
                     QAPSGQANSPAAAPEAHNNGWRRFGEPRVASPPNSRPAAQDPGGNLRSPGNQHSFGPG
                     SAPNNFAPAFRPSPGQYPAPQHSAPPVEPSRPAPQYSRPQASPWFGGGGGRQLQIQRP
                     ILAPRPGPAPSSGGSPRFGGGGSPHFSGGDRGGGGGQRGGNQHGGGGHSRR"
     gene            18285..19175
                     /gene="lpxC"
                     /locus_tag="VFA54_00255"
     CDS             18285..19175
                     /gene="lpxC"
                     /locus_tag="VFA54_00255"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZS49261.1"
                     /translation="MRFETTTQRPVEVAGVGLHSGVQVSIRILPAAAGTGIVFVRTDL
                     DGFSIPASWKYVARVSYATSLMRQGVLLSTTEHVLSVFYGMGIDNAVVEINSLEAPIL
                     DGSSRPFVELLQQAGRRRLRRERRYLRIRRAVEVRDGRRRITILPADGFRITCSTDFG
                     HPLAGRQEIDLDLTPERYEREIAGARTFGFERELDQMRDMGLIRGASLSNAVCFTSGG
                     VLNPEGLRYPDECCRHKVLDLIGDLALIGHPLVGHVIAERAGHAMHTALVAQIMNDRS
                     TFEIVTLDHLATEAVRTLVG"
     gene            19435..20700
                     /locus_tag="VFA54_00260"
     CDS             19435..20700
                     /locus_tag="VFA54_00260"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZS49262.1"
                     /translation="MPAVSPDYEKNAWASQSARFEEAAALLDLDEGLTKILRTPTREI
                     TVAIPVQLDDGRLEVFTGYRVQHSIARGPAKGGIRYAPDVTLDEVKALASWMTWKCAV
                     VNIPFGGAKGGIICDPMQLSPFELERMTRRYTAEILEFIGPERDVPAPDVNTDEQVMA
                     WVMDTYSMHTRQTTTAVVTGKPIDLGGSRGRREATGRGCMIVCNEALKKLGMAQAGAR
                     VVIQGFGNVGSMAAQQMAAHGYKIISVSTIDGAIYNSQGLDIPALIAYRAKHKTILGF
                     PEAEQIDAQEALLLDCDILLPAATENQITSHNAGKIKAKILCEGANGPTTADADPILR
                     ENGVFVIPDIMANAGGVTVSYFEWVQDRQGYFWNADLVNNRLQQIMTESFHDVLHYAE
                     KHKVHNRTAAYMLAIDRVAFAIKLRGIYA"
     gene            complement(20751..21209)
                     /locus_tag="VFA54_00265"
     CDS             complement(20751..21209)
                     /locus_tag="VFA54_00265"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZS49263.1"
                     /translation="MIGTVLSFFAGLLPVGTEAPDFSLRDDAGNLVHLAGWKGRAPVV
                     LVFYPVDETPGCRKQLCELRDHWDEFARRGVQVFGINPGSAESHGKFRRNHHFPFPLL
                     VDQGKKVAKLYGASGLVIRRTVYAIGKDGRIAFAERGKPDPGKILAAIAG"
     gene            complement(21206..>21757)
                     /locus_tag="VFA54_00270"
     CDS             complement(21206..>21757)
                     /locus_tag="VFA54_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49264.1"
                     /translation="LGSTQVAINGRLAPLLSVSPAQINLQVPFDITGTEAEIAVSTPA
                     GSSSPVKVPVIDIQPGIFFDPASGLGAILLNSNSASTAVQPARAGDFLQIYATGLGPV
                     LPTAVAGFPAAFTPPVRTTTLPEVTIAGFPAPVLFAGLAPGFAGLYQVNVQVPESVPP
                     GRQNVVLSMNGLQSNTVQVNIGP"
BASE COUNT         4234 a         6624 c         6682 g         4217 t
ORIGIN      
        1 aaatggacct gctgatctat ttgcctgtcg ccgcccgaac ccccacgccg ctgttgctga
       61 acttgagctt ttcggcaaat tcgagcgtcg tggatgatcc cggcgtcaag ccgggtgaaa
      121 tctggagccg ggagaagaaa aaaatcccgg ccagctcggg gaggagcttc ggcaagctca
      181 atatcatgcc gtttttggag cagggaattg gcgttgctac agtgtattac ggcgacatcg
      241 aacccgattt tcctgaagga attgcctatg gagtgcgaaa gttatacttc cagccggggc
      301 agagcgagcc tgcgccggac gagtggggag cgatcgcggc ttgggcctgg gggttgagcc
      361 gggccatgga ttacctggaa accgataagg ccgtggatgc gaaacgcgtc gccatcttgg
      421 gcgtctcccg cctcggcaaa accgtgctct gggcgggcgc tcgcgatccc cgctttgcgg
      481 cggtgattgc cagttgctcc ggagaggggg gagcggcttt gagccggcgg aattacggcg
      541 aaaccatcaa gcatttgaat gcgcgttttg ggtatcagtt ctgcgggaat taccagaaat
      601 acggcgatca cgtggaccag tttcctgttg atgcgcatat gctccttgcc ctgatcgcgc
      661 cccgcccgct ctttctccag accggcgaca aagacttgtg gtcggatcca aggggcgaat
      721 ttctcgctgc cgtggcggca agtccggtat accggctgct cggaaagcag ggactgggaa
      781 cagatcacat gccgcccgca ggtcaggcca tcctgcacac tctcggttat gagatgcact
      841 ccggcgggca tggagccctt ccgtcggatt ggaagctctt cctgaagttc ctgcaaatac
      901 agttgctagc gcggaagggc tagagcgctg cttcaacggg cagcggcgcc agcgaattga
      961 cgttgacgtc ctggtgaagg aggtttgcga ccgtaaagag accagaaatg gccgacgcaa
     1021 tcggtggccc tatttttagg cttgcaagcg gaacactcgg aaccggagaa gcgtccggag
     1081 gctctactcc gggcaggggg acaaaacatc ggctgcggag tactcccccg gataggctgg
     1141 attccgcgaa tgagatgtat tcgccgggtt ccaccgggcc attggttctg cggtattccc
     1201 ttagtccacg ccagaggacc ggagagcagt cgctttccgc ggttggcggg cgccggtttt
     1261 atccggcgcg caaggccgct ttggtcagct cacgatattt gcgcagcagc tcataagcta
     1321 caatgccccc ggccaccgcc acattaagag aatgtttccg gccgagcatg gggatgcata
     1381 cgtggacgtc gcactgctcg ctgagttcac gccgtatgcc ctcgatttcg tggccgaaca
     1441 caacgcagag cggaaagcgc ggctgccatt caaacaaatc caccgagcgg acggaggtct
     1501 cgatcgcggc catttcgcag ccctcttcgc ggattcgttg aagcgccggc gccaggtccc
     1561 aatgatgttc ccaggaaacc gtctcctgcg cgcccagtgc cgttttggct agggcgcggt
     1621 tcggcggatg gccggtaatt ccgatgagat acaagcggga caggccggcg gcgtccgcaa
     1681 tgcgaaagaa cgcgccgacg ttgtaaaggc tgcgtacctg atcaagcagt atcgaaacgg
     1741 gcaggcgcgc aatgccgaga ttagagcggg cggcggccgt agcgggatac ggaagccgtt
     1801 cttcctggcc gctcatctgg ctttggaccg cttcgagcgg cgctccagaa actctcccag
     1861 aggcacgccc agaagcatga acacgacgaa tatcggcatg gccctctggc cggaaatctc
     1921 caggcagcgc gtcaccacca gcgccgcaat ggtcatgccg atgatggacc agaaggtatg
     1981 gataggtgtg gtatggttca ttccgtgcga gtcacgtatt atcgctcaaa cacccgtgga
     2041 gccgaagccg ccggcgccgc gggctgtggg ggtcagcgcc ggctcgattt tccattccac
     2101 cgccgcatac gatgcgatga caagctgggc gatgcggtct cccggccgga tgtggaactc
     2161 ctgccggccg tgattgacca tcaagatccg gacttctccc cgatagcccg gatcgatcgt
     2221 gccgggcgag ttcaatagcg tgaccccatg ccgcaaggca agcccgctac gcggcctaac
     2281 ctggccctca tagccgggtg gaatctccaa tatcagtccc gtcggcacga cagcccattc
     2341 tccgggcttc aggcgggcct cctcggccgc atacaggtcc atgccggctt cctcttggga
     2401 agaatgcgca aaccgaggga gttgcgcccg aggatggacg aggcgaacgg aaatggtcac
     2461 tggcgtcatc gcctaagttt ccccgcttcg gggtattgtt ggcaactcgt tcccgccgat
     2521 aaacaagtcg gatgatctca atccgcagct atctggagaa gctggccggc gaggagggcc
     2581 tgcgctccgc ttttctcaag ctgatccaat cgcttgtcag ggtgatgcag gagaaaatga
     2641 tcaaagcgga cgccgaggag cacaactggc tctgcgcgaa cctggattcc ttgcagcggc
     2701 gcctcatgac tgatacaagc ccaaaaaaca tcgaagtggt cgcagctctg ctcacaaaaa
     2761 ccctcgaaga tcactggctg aagctgggca tccagtttga caaccggcag cacgagttca
     2821 aacgcatcat taacctgctg accgagagcg ccgcccggct cgacggccag aacaagcatt
     2881 tctacctctc gctgcgcgag gccgtccaaa attttcagga catatcccag atggaggata
     2941 ttacctacgt ccgcagaaag ctaaccgaac aggtcgcgaa tcttgaggaa accgtccagc
     3001 gccaggaagg agtttctaac gaaaccgtcg gccggttgca aagcgagctg gagcaggcgc
     3061 ataggcaaat tgcttcgctg atcgaagccg tcactacgga ttccctcacg cggcttcccg
     3121 cccgccaggc cgccgagaag gcgctacggg agcacatcga aaacaacgag ccgtttgtgc
     3181 tgggaatggc cgtggtggag aggttggagt tgatcaatct gcgctacggc gtggatagcg
     3241 gcgacgaggt ggtgcgaaag tttgcccggc agcttcggga aaatatgccc gaacgcaccc
     3301 tcttataccg gtggggcggc ccggcctttc tcagccttgt tccgcgcatg ggcgccggag
     3361 aattgcgcgt gttgctgcaa aaggtgttgt ccgagatcgc cgccgaaccc atagagctcg
     3421 aggccaaggg cgccggtttc atccggatca cgtcccgttt cgccgtccac gaatggacgc
     3481 cggggcaggg cgccgaaaag atggtccggc tggtcgatat tttttgcttc tcgccgaaag
     3541 gagaaagcgg ggatgcggat tcccgccaca tcagtaaggc cgcgatctga atcggagttc
     3601 acccgctgct ataatccagc cgatgtcctc cactggcgcc aatcgtgtga tcgcggtgtc
     3661 ccctatgccg ccggaaaaat cggcgtatgc gctcgcgcgt tatagccgct ctccggattc
     3721 gatttcccaa agccttgatt gggtccgggc gcacgactcc gcgaagttcc tggaatccta
     3781 ctacttccaa tacggccatg cttcgattgc cgacctgggg catacgatct tttgcttcga
     3841 gggaatttca gaactcgcgg cagtggagat tgaagatgag ccgctgtggg acggccaggc
     3901 taaatccagc cggtaccagg acttctcccg atccggattt gtggttcctc cggaaattgc
     3961 agagaccgga ggggcagccg tttacaaaga cacagccggc tgtctgctgg aagcgtatct
     4021 gggaatccat cagagagctt tcgattacct cgttgagcga acgcccaaac cggaaagcat
     4081 gaaggccgag gcgtatcgcc gcgcgctggc tgcccgggcc tttgacgtcg cccgctacgt
     4141 tttgtttctc ggcattccga ccaatgtcgg ccaggtcacc agcatccgga cgctcgaaaa
     4201 gcaaatccgg cgcttgagcg tgagtagata cgccgagatc cggcagatcg cggcggagat
     4261 gagggacgcg tgcgcacaac cgcctgtttg cgtctgggat gcggctagtt cccgggaacc
     4321 gctcgctcct acgctggcgc gtcacgccga tcccgacgtc tacctccggc acgcgcgccg
     4381 caatgtggcc gagtgggcgg cgcagaacct gccggcagga gctttttcga ctgttgcggc
     4441 ggagtcatcg gtggatctgg tgcgcccgcg cgatgccgtt gaggagattg tggcgacgct
     4501 cctctattcg gtcacgcaat ttccattccg cgcgatttac gaccaggtgt ccgggtggag
     4561 tgcctcccgt aagcgcgagg tgatcgacgc ggctgtgcgg gacagaagcg gaagggaaga
     4621 gctgcttcgc gaattccgaa gcggctacgc gtttgttttc gacatcgtca ccgacatcgg
     4681 cgcgtatcgc gacttgcacc ggcatcggcg gtgccagcag atccgccagg atttcacggc
     4741 ggagttgggc ttagagacgc cggccctcat tgctgaggcg ggcgcccaaa atatcttcca
     4801 tcatgcttta catgaggccc gccttgcgcg ggagcagctc gccgcagttt cgccggaagc
     4861 aacccattac ctgctgccgt ttgcggcccg cgtccgcttt ctctacaaga tggatttcgc
     4921 cgaagccgaa tacgtatccc ggctgcgatc cggggtcaaa ggacacttcg cctaccgcgc
     4981 gatcgcctgg aaaatgaagg aaaaggtgat ggaggttgaa ccggatctcg gccgcctgat
     5041 cgaggctacg ccgccgtggg tcgaagatcc attgatccgt tgaggcgacg gtttgcttcg
     5101 ccccttcccg cgcagggctg atcttcgacc aagccgccgg cgccggattc aaaccgcgac
     5161 cgcggcccgc tcctccttcc agatcttcaa gtacgggcca aggtcctgca tcatgcgctc
     5221 gaactgctcg atcgtgagcg attgcgcgcc gtcgctgaac gcctcctccg gacagggatg
     5281 cacttccacc atcaatccgt ccgcccctac cgctaccgaa gcccgcgcca gcggcggaat
     5341 caggctgctc ttgccggtgg cgtgggccgg gtcgagaacc acgggcaggt gcgtgagctc
     5401 ccgcagcacc ggtacggctc ctatgtcgaa cgtgttgcgg gtggccgtct caaaagtgcg
     5461 aatgccgcgc tcgcaaagga tgacatccgg gttgccgtgg gacgtgacgt actccgcggc
     5521 cagcagcagc tccttgattg tcgagctaag cccgcgtttc agcatcactg gcctgcccgc
     5581 tttgcccaaa gttttcagaa gagcgtaatt ctgcatgttc cgcgcgccga tctggagcac
     5641 gtccgcgtac tcggccacca gtccgacatc gccatcgctc ataacctcgg tgatgatgga
     5701 aagtccggtc tcctcccgcg cgcggcgcag cagcttcaac ccctccaatt caagtccctg
     5761 aaaatcatag ggcgaggttc gcggcttgaa cgcccctccg cgcagcagct tggctccggc
     5821 gcgcgccacg ccggcggcgg tggtgaggat ctgcttctcg ttttccaccg agcaggggcc
     5881 cgcgatgacg atgaattcct cgccgccgat ttccgcgcca tccactttga tctgcgttct
     5941 ctctttttta tattcgcggc tgacaaactt gtaaggagcc gaaatgcgaa cggcgctctc
     6001 cactcccggc gctgcttcca gggattccag acagtgggtc acatcgccga tgcccacggc
     6061 ggcgatgacc acccgctcat cgccgcggat ggaatgaacc ctgtagccga attcctcgat
     6121 ccggcggcaa acatgctcga tttgatcttt tgtcgctcct ggcttcattg aaatcaccat
     6181 gacccacctc gaaaaaataa gttgattgca aataaaaaac ccactctctc gagtgggttc
     6241 agtttgtcga tccaagcagt tacgaaaacc tacgccactc tacgggccgc tgaagcccgt
     6301 aaatcgaaac gaccaataat aggcgtaggc cgctcgcata gaacttttat tatgcgttgg
     6361 agcgcccacg gttgtccata agaatttggc gccgcactcg ccctgcgcgc caccactcgg
     6421 tggtgctgcc caatcgagac tctgtgcgtc acgccgcaac gattgttttg tcaacgacca
     6481 cagaaaatcg gacggcgtcg aaccggaaca agacgaaatc tgccatgtct ccgggctcca
     6541 gaaagcccat ccggccttcg aggcggacgg cccgggccgc gtttatgctg gccatccgcc
     6601 aagcctggtt caggctgatc ccggtgaagc gcattacgtt tgccactgcg cggtccaggc
     6661 gcagagcgga gccggccaaa cgctgcgatc cggcaatttg cacgcgccct tccggggtca
     6721 gctccaccag cacttccccg agccggtatg ggccgggcga acatccggcc ggagagctcg
     6781 cgtcggtgac cagcacactc cgcgtaacgc ctttcgcccg tatggcgcat ttgaggaatg
     6841 cctgcggcag gtgaatgcca tccacaatga agctggcata aagctcatcg gcggccatct
     6901 gctcccaaat gtaattggga tgtctgggca gtatcgggtg tgcgccgttg ccgaggtgcg
     6961 tggacatggt ggcccccgcg cggatcgcct cgcggatctg ctccgggctt gcgcccgtgt
     7021 gccccaggct gaccaccaca ccttggtcga ccagaaactc gatcaattcc gtagcccccg
     7081 gtgtttcggg agccagcgtg agcaaccgaa tgtttccttc cgccgcttcc tgcatggcga
     7141 ggaattcttc gcggtccgcg ggccgtacgt gctcgcgcgg atgggccccg cgcggtccat
     7201 cgtccggcga aataaacggc ccctccacgt gaatcccggc gatggaggct ccttggggga
     7261 tttcgcgctt cgcccgagcc aggttgcgca acgctccgca catccgctcg cgcgggcccg
     7321 taatcacagt cgggagaaac cgcgtcacgc cggtggaatg gatcgcctca atcgaacggc
     7381 cgatttcttc aagagacgtg ttgagcgaat tgtagtccac gccggcaaag ccattgacct
     7441 gcaagtcgat tagccccggc gcgacccaga ctcccgcggg gtctgcttcg ctctcctgca
     7501 cggcggtgat ccgcccaccc cacgtctcga tctcgaccgg ccgtccgcta tcggccgaga
     7561 tccccaggac atgttccctt acgcttgttt tattcacagg atttctacag taggctcatg
     7621 gcaaaatgcg cctttcacaa gataacaaag ttgtggataa cctcacggca cgggccgatt
     7681 tttgtttgac aacgtcgcgg cacagcggta taccaagcaa cggttccgag aggtcatcgc
     7741 ggtggatcct cagtttttcc agggcgtgcg cgtagcggag catggatcct tggcggacgc
     7801 cgaggggagt gaagcagcga gcccggaaaa ctgcccgttt ccgttcttgc accggagtcg
     7861 cgctatgaat ccggacggat ctcctccgtg cggaggagca agacgccggg cagcggcggg
     7921 gagcgacata gaatctgcgg gtgcaaggcc cgtagtgcga tgtttgctcg gctggcaacc
     7981 atcggccggg agcgtcgatc ccttctgggg gagcgaaagg ccgcggaagc cgccaggcgg
     8041 gcgcagaggc gccggatgat cactcggaac catctttatt cgtcaatcca aatcgtaacc
     8101 tccagccggc ggttcgcgcg gcagacatcc catgctcgcc accctttacg gcattccgat
     8161 cggctatgac gtgaccgggc agggaattcc cttgatgttt ttgcacgcat tcccactcga
     8221 ccggtcgatg tttcgcccgc ttccgggagc acgcctgata acctttgacg cgccaggggc
     8281 gggggagtcg ggtctgccgc ccggagatct cagcatggag gctgtggccg acatcgcagc
     8341 cggactgctc gacctgctcg ggatcgagaa ggccgtagtg ggcggcatct cgatgggcgc
     8401 atatgcggcg ttccgcttcg tttgccggca tccacggcgg cttctcggcc tgattctcag
     8461 cgacacgcgg gccgcggcag actcggccga agggcgcaag gcgcgctacg agatgatgga
     8521 ggccgcacgg tccttgggtt ccgccgagat tgcgcgccgc atgctgcccc gcttgctggg
     8581 tgagacaaca cggcgcgaaa acccggccct ggtcgagcag gtacggctca tgatcgggaa
     8641 acaagacccg gaaggcatcg cgcgtctttt ggaagctctg gctgaaagga ccgactcgac
     8701 cgctctgctg aagcaaatca cggttcccac cctcgttgtg gccggtgagg aagatgtcat
     8761 tgccacggta gcggagtctt cagaaatggc gaagaggatt ccgggcgccc ggctcgccat
     8821 catccccaag gccggacatc tccccaactt ggaacagccc gagaggtttg aaaaagccgt
     8881 agagagcttt ctgcaagaga ttgggcgtga ataaatgcgg gcggaagccc acccggcgcc
     8941 agattgaggc cgccgatccc ggcccctcag gctggtgcca gttagcagct agccgacgat
     9001 tgcgaccggt tttcgcgcgt ttacattcgt gagccagcca tagggatcgt tgccttgaat
     9061 gaccgaaaag aatctttttt gcagccgctc ggtgatcggg ccacgccgcc cgctgccaat
     9121 ctggatccgg tccaccgatc ggatcggagt gatctctgcc gccgtgcctg tgaagaaaat
     9181 ttcatcggcg atgtacaaca tttcgcgggg aatcgcttgt tcaatcactt caatattaag
     9241 atcgcgcgcc agccgcaaaa ccgtgtcccg cgtgatgcct ggcagcgccg aggttcccag
     9301 aggcggcgtg tagaggtgtt cgtcgcgtac cacgaagatg ttttcgccgc tgccttcgct
     9361 cacgtagccg gcaacgtcga gcgcaatgcc ttcggcgtag ccgttggtga tggcctccat
     9421 tttgatcaac tgggagttca tataattggc cgaggccttg gccatcgccg gcaacgtatt
     9481 gggcgcgagg cgagtccagg aggagacgca gacgtccacg ccctcggcca gcgcttccgg
     9541 accgagatac tttccccatt cccagcacgc gatataaacc tcgatcggat tgtggaaggg
     9601 cagcactccc atctcgccgt agccgcgaaa ggcgatcggg cgaatgtagc agctatccat
     9661 gcgattcacg cggaccagtt cgaccatggc ctcgatgagc tgagcgggcg taaagccgat
     9721 ctccatgcgg tacacccggg ccgagtccgc cagacgccgg atatgctcgg gagcgcgaaa
     9781 gatcgccggc cccgctggcg tcgagtagca acggattcct tcaaacacgc tcgagccgta
     9841 gtgcagcgta tgcgacagaa tgtgcacccg ggcgtcatcc cagttgataa attcgccatt
     9901 gtgccagatc ttctcggtcg gcttgatcat tggtccccca gtgtcgcaat tataacacgc
     9961 aggtcccgaa accttcggcc gaaccccgga ctgactggcc cgtggcatgc agagggaatt
    10021 tgttaacctg tttttcaggg aacaggagac cggacgacaa cagcttttcc ttcccaagga
    10081 ggccgtgatg cgaaaatttc acgtgtttgg ttgccttggc gtcttgtggc tggccgtatc
    10141 agcggccgcg gcccctcggg aaatcaagcc gggcttcaat ctgttttcgc gcgatcagga
    10201 cattcagttg ggccgcgagg cgcagcagca ggtagagaaa gaggtaccag tagtacacaa
    10261 cgcccccgag ctggaaaact acatttctca gctcgggatg aagctcgcca aggtttccca
    10321 ggcgcccgat tatccctaca attttcacat cgtcgccgac aagaacatca acgcgttcgc
    10381 gctccccggc gggccggttt acgtgcacac ggccaccatc gccgccgccg ataacgaggc
    10441 ccagcttgcc ggcgttctgg cccacgagat ctcgcacgtc gcgctgcggc actccaccaa
    10501 ccaggcctcc aaggcctacg cctggcaaat accgctggca atcgccggcg gagcggccgg
    10561 cggctcactg ctgggccagc tggcgcagct cggcatcggg ctgggcgtga actcggtgtt
    10621 gctcaaatat tcccgcgacg ccgaacacga cgccgatatc gtgggcgcgc agactatggc
    10681 cagagccggc tacgacccgg tggaaatggc tcgcttcttc gagaagctaa aatcgcaggg
    10741 cggtggaggt tccgcgcttc aattcctctc tgatcacccc gaccccggca accgcgtgca
    10801 atacgtcgaa gctgaggtca agacctttcc gccccggcaa tacaccaaag ggtctccgga
    10861 tttcgcgcgc tacaggcaaa tggcggcgca aatccccgcg ccccagctaa agcgcagcta
    10921 caacgaccag ccgagcggaa accatccgca cgcttcattt ccgtcttcgg atctgcggac
    10981 ctttcgcggc gccggctttc acctgagtta tccgagcaac tggcagcctt tcggcaacga
    11041 gaacgaggcg gcaatcacca tcgcgccgcg cgacggcttg gtgcagggaa gcgagggagt
    11101 gcagatcggc ctgggaatga tcgctggata tttttcgccc gattcgaata acctcacgac
    11161 ggctaccaac cagttcgtgg aggacctgca agccaagaac gccggcctgc ggcccctgcg
    11221 ggggcagcgg cgcagcacaa ccgtcgatgg cgtgaacgcc gaaacgctcc tgctaagcgg
    11281 cgcctcgccg ttgggcaggc agcgggaaat cgattcgctg ctcacggcgc tgcggcccca
    11341 gggccttttc tatctggtgc tgatcgcccc ggaaagcgat tacaacagcc tccgccccat
    11401 attcgtgcag atcgaaaatt cggtacgatt ccagtaggga tgatccaggc aatcttcttc
    11461 gactttgatg gagttctggt cgatagcgag ccgctgcatt ttgcctgctg ggccgaagcg
    11521 ctcgcccagc acggcatcgc gatcaccgcc gaatcctatt accggcgatt taccggcgtc
    11581 agcgaccata gcatgatcga cgccctgtgc cgagagttcg gcgccgaggg cgaccgcccg
    11641 ctgtttaccg cctgtttcca ccgcaaaaag caactatacc gtgagcgggc cgcggagttc
    11701 tgtcacatac cgcccgagct tgtaacattt ctggcggatt tgagcgccgc ttaccgtctg
    11761 ggcgtggttt cctccagcga ccggcccgac gtggagccgc acctggccgc ccacggagtc
    11821 cggcggtggt tccgagccgt ggtgtgccgg gaggatgtcc gcaaccttaa gcccgcgcca
    11881 gacccgtatc tgcgggcgct cgagctgctg aacgacggcg acggcgccgc cgtgaagtcc
    11941 gcggaatgcc tggtcgtcga ggactcggcg cccggggagg aggccgggcg gcaggccggg
    12001 atgcgcgtgc tgcgggtgcg caggcccgag gaggtggccg atgtgctgcg gcgcgagctg
    12061 gacggaacgt cggataagat atattatgtt aaataaacag gggcgccagc gcctctcctg
    12121 gatccgcacc tttttgacgg ccctccacac tgcccgctcg ccccgccccg gcccgcgccg
    12181 gttttcgcag tgactattct tccgaaatgg cgtccctggc tgcggctcgg ccgtatcgac
    12241 ttgatgtggc tgcttgtgat ggccgccctg gctgttctgg cttccacgcg tccgcgccac
    12301 agtccttacg agtttctggc gctcggggcg attgcgctaa tgcaggttgc ggaaatgcgg
    12361 ttcccggtgt ttgcgtcacg cggcggcacg attgtcgcgg tggttataaa gctcgcgctg
    12421 tgttgggttc tggtgggcgt gacggaaggc atcgagagca gctattacct catattcatt
    12481 cttccggtgg tctctgcggc ttccactttc gggttggccg ggacgctact caccgctttt
    12541 gccggatccc tggcctatct gtcatttctg gggtttgtct gggatcgcta cgagttgccg
    12601 cccgagggcg cccgcgagct gggcatccgg atcctcttca ttttcatgac ggggatcctg
    12661 ctgaaccatt tcgtcaccga gaaccgccgg caagccgagc tctaccgcaa gttggccgac
    12721 gacttggctc aggcgaaccg cgacttgcac caagcccagg cgcaggtacg gcggtcggaa
    12781 cggctggctg cgctcggaca gctttccgca ggcctggcgc acgagttgcg caatccgctg
    12841 ggagtgattc gcgggtcggc cgaactggtc tccaagaacg tctcggcgga aaatgaagtt
    12901 gtccgcgaaa tggcggcgtt gatcatcagt gaagctgacc gcaccaacgg tttgatcacg
    12961 cgattcctcg aattcgcccg gccgtcgccc ctgcaccgct gccaagccga cttgcacgcg
    13021 gtgatccagg aggcgatcca tcaggtgcag cgcagcctgc cctccggcgc aaacggcctg
    13081 cgcctcgaaa cggatttctg caaggacatg cagccgttct ccttcgaccc cgaattgatg
    13141 gaacgggtgt tcttcaatct gctggaaaac ggggtgcagg ctatgccggg cggcggagag
    13201 ttgaagatca cgaccaggcg gctcggcgac gtggtgcaaa cggaagtttc cgataccggc
    13261 gccggcatcg cgccggagaa cctcgagagc atttttaacc ccttcttcac caccaagcag
    13321 aacggcgtcg gcctgggctt ggccatcgtc tcgaagatcg tcgacgacca taacggcaag
    13381 attacggtct ccagccagcc gggccgcggg acgaccttta ccgtaattct tcccctttct
    13441 gaggcaagtt gaacatgaag aaacggctgt tagtggttga agatgaggcg aatatgctcc
    13501 gggtattaga gcttcagctt gctggcgccg gcttcgccgt cgaaaaagcc tccaatgccg
    13561 agcaggctct tgccattctc gagcgcggag gcctggatct ggtgctcacc gacctgcggc
    13621 tgcccggcat ggacggtctc caactgctgg agcgcatccg gggcatcgat gcttccctgc
    13681 ccgtgatcat catgactgct ttcggcacgg tcgaaacggc ggtccaggcc atgaaggccg
    13741 gggccagcga ttacatcctg aagccgttct cgctcgacga cctgatgatg accatcgaca
    13801 aggtcctcga gctgcacacg ctccgggatg aaaaccgccg cctgaaggac gagctcgggc
    13861 ataagtatca gttcgacaat atcattggaa agagccccaa gatgcaggag gttttcgctg
    13921 ctgtctcaag agtcgcgccg acgcgcgcca cggtgcttct cgccggagag agcggaattg
    13981 gcaaggattt gatagcccgg gcgattcacc accactctcc gcgccgcgac cgcccgttcg
    14041 tgaaaatcaa ctgctccgcc ctgccggaga acctcatgga aagcgagctg ttcgggtacg
    14101 aaaaaggcgc gttcaccggc gctgcttcgg ctcgtgcggg aaaattcgag caggccgacg
    14161 gcgggacagc gttcctcgat gaaatcggcg acgttccgcc tcccgtgcag gtgaagctgt
    14221 tgcgcgtatt gcaggagcgt gagttcgagc ggctcggcag caacaagacc ctgagggtcg
    14281 acgtccgggt gatcgccgcc accaaccagg atttgcgggc ggctttagag caaggagctt
    14341 ttcgcgagga cctctactat cgcttgaatg tcgtgccgat taacatcccg cctctgcgcg
    14401 aacgcaaaga agacattccg tacttagtcg agcacttcct gggcaaatac tccgaagaaa
    14461 ccggcgggcg ggtccgctca atcactccgg ccgcaatgga aaaactgatc gcgtatcatt
    14521 ggcccggaaa cgtgcgcgaa ctggaaaata tcatccagcg ggcgatggtt cttgcgccgg
    14581 ggccggccct cgagccgggg gacattcagc tcgactcgga gcgtcgcgcc gcagcaaccg
    14641 cggacggcgt tcccttcctt cccgaaggaa tgactctcga tcagtacgag cagcaactga
    14701 tccgcgaagc gctgcgccgt tgcgacggca ataaaagcca ggccgcccgt ctgctcggcc
    14761 tcacgcgcaa tgcgctccgg taccgcctca cccagatggg catcgaggcc tagacgctta
    14821 gcagactgtc atatcaagtt ttactttaag tgtggccggc ggcgcctgta cgagactcgt
    14881 tgcgcaaaag cttggcggaa gtcggcatcc cccataggac tgcccggcat gtaagagtgc
    14941 gtgccgattc attttcgggt ggactcgtcc aggtgaaggt tctcaagagc tcgggcgacg
    15001 tttttcgagt tatggaaacc cggagacacc gcttccagca ggggcgccag ccagatcgca
    15061 gccaacagga acagcgcggc gtgaatgctt cccatgggcc gcaaatcaaa gctgccatct
    15121 ttcgaaagca agttgttcag tttcagagcg ggcgcaatca gaagcaattc ggaggaccaa
    15181 aacgggaatc ccaatgatag cgttcatcgt aaaccaaacc gggtctcggc atagctgaac
    15241 ttgatgggtg aacctaaatt atagcggccc agcaacttga agaagtccgt atcgttagcg
    15301 attcctcggc ggaggaagag ttagttgcca aaacaaaaca aacgcggcgg cgcttatagc
    15361 tatggccgcg gaccggggcg cgcgcgcatg gtaatttcga agcggctgca ttgtttttcg
    15421 gccggcatgg tcgcaggcaa gagtgcggtg cacccgttca taaacaggtc gggaagagcc
    15481 tgcacccatc ctttcggcgc tttggctatt tgatttcatt attcggaaat gaaatcgtac
    15541 gcgcccgctc aacagccgtt ccttgcgatg gggacgggct gccagacctc ctggatttgg
    15601 ctctgtccgg ccgaaagcca ccagggcgtg gtggttttcg gccaggggat ccggtcggga
    15661 ttcatgatta tctctttttg tttgagaaca ttagaaaatg gaaggacggg tgcaagcaat
    15721 ctggacggga ggcgtccggt gcccactcga aagcacaaag cgatcgtgat cggctgcgcg
    15781 ctggcaatcg ccgccgcgcc gttcctcaca gcccagtcac cgagctatcc ctcctccggg
    15841 tacccttccg ggcagcagat cgccggtccg gcggtggccc gagtgagcct cttgaatggc
    15901 gaggtcagca cgcaaagagg cgattccgga gactggatcg ctgcggcggt gaacgcccca
    15961 ctggttgccg gggaccgcgt gtatgcggcg gccggatctc gggcggaaat tcagtttgat
    16021 tcagctaatt tcctgcgaat cggtcaagaa accgagatcc aattggctga tctcgagccc
    16081 ggccagcatc aagtccaaat ttcaaaaggc attattactt acggaatctt gcgaaattct
    16141 tcggcgagag tggaaataga tacgcccttg gtcgcgatcc ggcccaccga gcgcggcagt
    16201 tatcgcgtag aagtatcgga gaacggtcag acgcaaatca cggtgcgagg cggtagggcg
    16261 gaagtttctt cacgccaggg taccgaggcg attgaaggcg gccacaccct gatcgtccgc
    16321 gcagacgacc cgaatagcga cgtcgaagtg cgggacatgc cagctcccta ccccgatcaa
    16381 tgggatcgct ggaacgacag ccgggacgca cagttgaccc gcacggccag ctatcagtat
    16441 gtcaacccgg aagtgtatgg tgtagaagat ctggatgcgt atggggcctg gacccaagtg
    16501 cccggctatg gaccggtgtg gtacccccgg gttgcggcca cgtgggcgcc ctatagttat
    16561 ggccggtggt gctggatcga cggttatggt tggacctggg tggattacga tccgtggggt
    16621 tgggcgccct tccattacgg ccggtggttc tggcgcgcgg gcttcggctg gggctggtgg
    16681 cctggtccgg tgaccgtggt ttctccttgg gcgccggcct tggtagggtt tttcggattc
    16741 ggcgccgctt ccggtttcag cgtaggtttc ggcttcagca acatcggttg ggtgcccctg
    16801 gcgcctttcg agcccctcca tccgtggtgg ggacatggtt tcggcagccg gaccgttatc
    16861 accaacatca acatcgtaaa tgtaaccaac atccgcaacg tgtaccggaa cgcggccgtg
    16921 aatggcggcg ttcgggctat tgccgttcag gaatttaacc aggggcgcac cttcaaccat
    16981 atggcggtga atccggcaat gatcagcaac gcagctcaga ttcgcgggat gctcccggtg
    17041 gtccccagcc ggaacgctct tcaggtcagc acccatccgg tgaaccgggc ggccgtgccg
    17101 caatccggag cggcgcagaa ccgtttcttc gcagtgcatc aggctccggc tgtgcgatct
    17161 tcttttgtgc agcaacagca gcagattgca caggctttaa acggcgggag aatacaaaac
    17221 cggcccgcga tgcgtggtaa cacggggttc tccagcgggc tgccggcttc tccggcaaat
    17281 accctgcggt ctccgcaagg aaacaacctg gttcagggcc aatcgcagtt tcaggccgca
    17341 cccgcggctc aggtacgggg cggcgccacg ggaaaggcat cgggcggctg gcgctccttg
    17401 ggcccggcgg cttggcccag ccagagcggg ctggcgcaag cgccttcggg ccaagcgaac
    17461 agccccgcgg cggcgccaga agcgcacaac aacggatggc ggcgtttcgg tgagccgcgc
    17521 gtcgcttccc ctcccaactc ccgacctgcg gcgcaggatc cgggaggaaa tctccggtct
    17581 cctggaaacc agcacagctt cgggccgggc agcgcgccta acaacttcgc gccggcgttc
    17641 cggccctctc ccggccagta tcctgctccg caacattcgg ctcctccagt cgagccttcg
    17701 aggcctgcgc cgcagtactc ccggccgcag gcctcgccgt ggtttggcgg cggaggcggc
    17761 cgccagcttc agatccaacg tccgattctg gctccgcggc cgggaccagc tccctcgagc
    17821 ggaggctcgc ctcgtttcgg cggaggcggc tcgccccact tcagcggggg agaccgcggc
    17881 ggaggaggcg ggcagcgcgg cggaaatcag cacggaggcg ggggccactc acgccgctga
    17941 cgactttccc tcttcttccc tctgggcctg ctcggataca accagcaggc cctttttttg
    18001 ccaacccggt cttgatctgt gcacggctca gccgggctgg gggcactttc cgatcaagcc
    18061 gggcgggaag cctcgcccac ccaggagagt ggaaaggggc tcagtgggtc cgggttttcc
    18121 cgcggttcgg agtgtggctt atcggccacg gttttgcccc gccggagtct cccggcagcc
    18181 gcacctgcgc cagcgattat ctcaataatt cagtgaatta cgatttggcg tgtaagatgc
    18241 tactgggcat tctccaaact tagcagtttc aggggagctt tggcttgcgt ttcgagacga
    18301 cgacgcagag gcccgtggag gtggcgggtg taggtttgca cagcggtgtt caggtcagca
    18361 tccgaatttt gcctgcggcc gccggcaccg gcattgtctt tgttcgtacc gatctggacg
    18421 gcttttctat tcccgccagt tggaaatatg tagcgcgcgt cagctacgcc accagtctga
    18481 tgcggcaagg cgttctgctc tccacgactg agcatgtatt gagcgttttt tacggcatgg
    18541 ggatcgacaa tgcggttgtc gagatcaact cgctcgaggc tcccattctg gatgggagca
    18601 gccgtccgtt tgtcgagctg ctgcagcagg ctggccggcg ccggctgcgg cgcgagcggc
    18661 gctatcttcg catccgccgc gcagtggaag ttcgggacgg ccgccggcgc attaccatac
    18721 tgcccgctga cggttttcgc atcacctgct ccaccgactt cgggcatcct ctggccggcc
    18781 gccaggagat cgatctcgac ctgaccccag agcgttacga gcgtgagatt gccggtgcgc
    18841 ggacgttcgg gttcgagcgc gagctcgacc agatgcggga tatgggtctc atccgcggcg
    18901 cttcgctctc caacgcggtt tgcttcactt caggcggcgt tctaaatccc gaaggtctcc
    18961 gctatcccga tgaatgctgc cgccacaagg tgcttgatct gatcggcgac ctggctctca
    19021 tcggccatcc attggtaggc cacgtaatcg ccgagcgggc gggccacgcc atgcacaccg
    19081 ccctggtcgc ccagatcatg aatgaccgct ccacctttga aatcgtcaca ctggatcatc
    19141 tggccacgga agccgtgcgg accttggtag gataggctgg tgtgtcgtgt cccaggttca
    19201 ccaacacagt gtcacggctc gctgacgcgc gctgcctggc aagcggccgg cggcggaagg
    19261 gccggcgctg tgagaacgat ggcggccgaa aaaagctgcc atcaaaactc tgagacacgg
    19321 cgctagctgg cggccagcgg ccgggacgcc gcgagaatcg gcttgcagca tttcgcctct
    19381 aggctccatg ccaacccgcg cccgccgcca tccaaacccg atgggaggct tttgatgccg
    19441 gctgtatcac cggactacga aaagaatgcc tgggcctcgc agtcggcccg cttcgaggag
    19501 gcggccgcgc tcttggatct cgatgagggt ctcacgaaaa ttctgcgaac gcctacgcgc
    19561 gagattacag tcgcgattcc ggtccagctc gacgatggaa ggctcgaggt gttcactggc
    19621 tatcgcgtgc agcactcgat tgcgcgtggt ccagcgaagg gcggcatccg gtatgctccg
    19681 gacgttacgc tcgatgaagt caaggcgctg gcttcctgga tgacctggaa atgcgcggtt
    19741 gtaaacatcc ctttcggggg cgccaaaggc ggcatcatct gcgatccgat gcagctttcg
    19801 cctttcgagc tcgagcgcat gacgcggcgc tataccgccg agatcctcga atttatcggt
    19861 cccgagcggg acgtgccggc gcccgatgtg aacaccgacg agcaggtcat ggcatgggtg
    19921 atggacacct actctatgca cacccgccag accacgactg ccgtcgtaac cggcaaaccc
    19981 atcgatttgg ggggctcgcg cggccgccgg gaagctaccg gacggggctg catgatcgtc
    20041 tgcaacgaag cgttgaagaa gctgggaatg gcgcaggccg gcgctcgcgt ggtgattcag
    20101 ggatttggca acgtgggatc catggccgcg caacaaatgg ctgcccacgg ctacaaaatt
    20161 atttcggttt ccaccattga cggcgcgatc tacaattccc aggggctcga cattcctgcg
    20221 ctgatcgctt accgcgcaaa gcacaagacg attcttgggt tcccggaagc cgagcagatt
    20281 gacgctcagg aggcgttgct gctcgactgc gatattctat tgccggcggc gaccgaaaac
    20341 cagatcacca gccacaacgc agggaagatc aaagccaaaa tcctgtgcga gggcgcgaat
    20401 ggtccgacca ccgccgacgc cgatcccatt ctgcgggaaa acggcgtttt tgtgattccc
    20461 gacattatgg cgaacgccgg cggggttacg gtttcctact tcgaatgggt gcaggaccgg
    20521 cagggctatt tctggaacgc ggatcttgtc aacaaccgcc tgcaacagat catgacggag
    20581 agtttccacg atgtcctgca ttacgccgaa aagcacaagg tgcacaaccg gaccgccgct
    20641 tatatgctgg ccattgatcg cgtcgccttc gccatcaagc tcagaggaat ttacgcctag
    20701 ctcgcgtctt gccggctggg cggacttgaa gatagttcct ccgctcctgc ttagccggcg
    20761 atggccgcca ggatcttccc cggatccggc ttaccacgtt cggcgaaggc gatgcggccg
    20821 tccttgccga tcgcgtaaac tgtgcgccgg attaccagcc cgctcgcacc gtagagcttc
    20881 gccactttct tgccttgatc gaccagtaaa ggaaatggaa aatgatgatt ccggcggaat
    20941 tttccatggc tctccgccga gccgggattt atgccaaata cctgcacgcc gcgccgcgca
    21001 aactcgtccc agtggtcgcg cagctcgcaa agctgtttcc ggcagccggg cgtctcgtct
    21061 acgggataga acacaagcac aacgggggca cggcctttcc agccggcgag atgaaccaaa
    21121 ttcccggcgt cgtctcgcag ggaaaaatcg ggagcttccg ttcccacagg aagcaggccg
    21181 gcgaagaagg agagcacggt accgatcatg gtccaatgtt aacctgcacg gtattcgatt
    21241 gcaggccgtt catcgagagc acaacgttct gcctgccggg agggacgctt tccggtactt
    21301 gtacgtttac ttgatagagg ccggcgaacc caggcgccag gccggcaaag aggaccggcg
    21361 ccggaaaacc ggcgatcgta acctcgggca acgtggttgt ccgcacaggc ggagtgaacg
    21421 cggcaggaaa gcccgcaacg gccgtgggca agactggccc caacccggtg gcgtagattt
    21481 gcaggaaatc tccggcacgc gccggctgca ctgcggttga agcgctattc gagttgagaa
    21541 ggatggctcc caatccactc gccgggtcga agaaaattcc cggttgtatg tcgatcacgg
    21601 gaaccttcac cggggaagac gaccccgccg gggtcgagac ggcgatttcc gcctccgtcc
    21661 cggtgatatc gaagggaacc tgcaaattga tctgcgccgg ggaaacggaa agaagcggcg
    21721 ccagccttcc gttgatggcg acctgggtgg atcccag
//
LOCUS       DASREK010000005         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_331577, whole genome shotgun sequence.
ACCESSION   DASREK010000005 DASREK010000000
VERSION     DASREK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331577"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /gene="gpmI"
                     /locus_tag="VFA54_00275"
     CDS             complement(<1..828)
                     /gene="gpmI"
                     /locus_tag="VFA54_00275"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZS49265.1"
                     /translation="MTRPRPLVLTILDGWGYSPETKGNAIALARKPIYDFLLRQYPNT
                     LIHTSGPYVGLPEGQMGNSEVGHLNIGAGRVVHMDVTRIDLMISSGEFFRHPVLLEAM
                     HHARNHRLHLLGLCSQGGVHSQLTHLYALLKMAKAEGVKDVFVHAFLDGRDTPPQSGV
                     HFIAELEQQMRTIGAGRIASLSGRYYAMDRDRRWERIERAFRAIVLGDGIRATDACQA
                     IRQSYERGVTDEFLEPVVITTAKGDPVGTIRDDDSVIFFNFRADRARQMTRALMDPAL
                     "
     gene            886..>3352
                     /locus_tag="VFA54_00280"
     CDS             886..>3352
                     /locus_tag="VFA54_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HZS49266.1"
                     /translation="MHDGKWRFRFVACIGVVFAASSLQAQRRPDYNFYDSRTWNRISA
                     AGAASAARRALAAEFGEDVTAVPNQPGGSIAEIYRLAGALTAPAAADPETIARAFLQF
                     HRAPLALAGTSVFSLPLERRIESAASGLTHLVFQQRYAGIGVFGGEVRVHLTRDGSIL
                     RVQSGAGWPSQANPALAAPLGAADALAAAARVLLPDVPGDEPPRAAAPGVHVLSPESG
                     AERQTVLAASGFADPIPAKLVWFPRTNDAVLAWELYLHVGPARWYCAVVDAATGELLF
                     SHNMYRDQNPRGSVFRAPDVSNPSLGAQTVENFTGWPAAEGNCPASIYPPQYRSGPQV
                     NQCWVSGTQTSGNNVVACLDASGANRCDWQASNAQADFEFAFTDGYAAMGNAVPDQSA
                     AVTNLFYWTNVIHDWLYSLGFDEASGNFQADNFGRGGFGGDPVLADAQDGSGLNNSNF
                     ATPPDGSSPRMQIFLFTNNGTYVRRDADFDGDVITHEYVHGLTTRLVGGAGNVNVLPR
                     WQSGGMGEGWSDAYACSLMNNPVFAPYISATPSVGIRSVAYNNSPYTFGQFGTLYRSS
                     VGAWLLDLPEVHRDGEIWASLLWDLRTVLGKAVFEPLVTTALKLTPPRPSMIDARNAI
                     LQASQVLGASSCDVWTVFARRGLGASAALNHIEAGESADTALSVYEAYDLPAACGGSP
                     PAPGATIFSDDMETGAIGWTATGLWHLTARRAASGAHSWWYGQESTGNYSTGSANSGS
                     LTSPPIAIPAGAKVILEWDQYFQGEGFGRCYPFAGQGCNPYVNYDSGWVKASVNGGST
                     WFTLTTIAHNSDSAAFDHYKLDLS"
BASE COUNT          583 a         1064 c         1035 g          670 t
ORIGIN      
        1 gagggccgga tccatcaagg cccgagtcat ctgccgggcc cggtcggcgc gaaagttgaa
       61 gaaaatcacg ctgtcatcgt cgcggatcgt ccctaccgga tctcctttcg ccgtggtgat
      121 cacaaccggc tcaaggaatt catcagtcac gccgcgttca taggattgcc ggatcgcctg
      181 gcaggcgtct gtggcgcgga tgccgtcccc caagacgatc gcccggaacg cgcgctcaat
      241 ccgttcccag cgtctatcac ggtccatggc gtagtagcgg ccgctcagcg aagcgattcg
      301 gccggcgccg atcgtccgca tctgctgttc gagctcggcg ataaaatgca ctccgctttg
      361 cggcggcgta tcgcggccgt ccagaaaggc gtgaacaaat acgtccttca cgccctcggc
      421 cttggccatc tttaaaagag cgtacaaatg ggtgagctgg gaatgaacgc cgccctggct
      481 gcacaagccc agcaaatgaa gcctgtgatt gcgggcatga tgcatcgctt cgagcaaaac
      541 gggatggcgg aaaaactccc cggaactgat catcaggtcg atgcgcgtta cgtccatatg
      601 aacgacgcgc cccgcgccga tgttcaggtg ccctacctcg ctattcccca tctgcccttc
      661 gggcaagccc acgtaggggc cggaggtgtg gatcagcgtg ttgggatatt gccgcagtaa
      721 aaagtcgtag attggtttgc gcgcgagtgc gatcgcgttg cctttggtct cgggtgaata
      781 gccccatcca tcgaggatgg tcaataccag cggtctgggt cgagtcaccc taccgttata
      841 gcgtggattg accgatgaca ctacagggaa ctgttaccgc tcggcatgca cgatggaaaa
      901 tggcgcttcc gattcgttgc ctgtatcggc gtcgtcttcg ctgcttcatc tctccaggcg
      961 cagcgccggc cggactacaa cttttacgac agccgaacct ggaatcgcat ttctgcggcg
     1021 ggcgcggcga gtgccgcgcg ccgcgccctg gccgccgagt tcggggagga cgtgacggcg
     1081 gtcccgaatc agccgggcgg cagtattgcg gaaatttacc ggctcgccgg cgcactcacg
     1141 gctccggctg ccgccgatcc ggaaaccatc gctcgcgctt ttctccaatt tcaccgggcg
     1201 ccgcttgcct tggccggcac atcggttttc tccctgccgt tggaaaggcg gatcgagagc
     1261 gccgcctccg gcctgacgca tctggtcttc cagcagcgct atgccggcat cggtgttttt
     1321 ggcggggagg tccgcgtgca tctcacacgc gacggcagca tcctccgggt gcaatcgggc
     1381 gccggatggc cgtctcaggc caaccccgcc ctggctgcgc cgctgggcgc ggccgatgcc
     1441 ctggcggcag cggcccgagt gcttctgccg gacgttcccg gggatgagcc gcctcgcgcg
     1501 gcggcccccg gcgtgcatgt tctctccccg gagtccggcg ccgagcgaca gacggtcttg
     1561 gccgccagcg gcttcgccga tcccattccc gcaaagctgg tttggtttcc ccggacaaat
     1621 gacgccgtgc ttgcctggga gctttatctg cacgtcggcc cggcgcgctg gtactgcgcg
     1681 gtggtcgacg cggcgacagg cgagctgctc ttcagccata acatgtatcg ggatcagaac
     1741 ccgcgtggaa gcgtatttcg cgccccggat gtctcgaatc cgagcctcgg ggcgcaaacc
     1801 gtcgagaact tcaccggctg gccggcggcg gagggcaact gccctgcttc catttatccg
     1861 ccgcaatacc gctcggggcc gcaagtcaat cagtgctggg tgagcgggac gcagacctcg
     1921 ggcaacaatg tcgtcgcctg tctcgatgcc agcggcgcaa atcgatgcga ttggcaggcc
     1981 tcgaacgcgc aggccgattt tgaatttgct ttcaccgacg gctatgcggc catgggcaat
     2041 gcggttcccg accagagcgc cgccgttacg aaccttttct actggaccaa cgtaattcat
     2101 gactggctct acagcctggg gttcgacgaa gcgtccggca attttcaggc cgataacttc
     2161 ggccggggcg gcttcggcgg agatccggtg cttgcggatg cccaggacgg atcggggttg
     2221 aataactcga atttcgccac gcctccggac ggctcgtcgc cgcgcatgca gatctttttg
     2281 ttcacgaata atgggacgta tgtgcggcgc gacgctgact tcgatggcga tgtcatcacg
     2341 cacgaatacg tgcatggtct cactacgcgc ctcgtaggcg gggccggcaa cgtgaacgtc
     2401 ctgccgagat ggcagagcgg cggcatgggc gaaggttgga gcgacgccta cgcctgttcc
     2461 ctgatgaata atccggtttt cgcgccctat atttcagcca cgccctcggt cggcattcgc
     2521 tccgtcgcct acaacaacag tccgtatact ttcggccagt tcggaacgct gtaccgcagc
     2581 agcgttggag cctggcttct cgatttgccg gaagtgcacc gggacggcga gatctgggcg
     2641 agcctgctgt gggacctgcg caccgtgctg gggaaggctg tttttgagcc tctggtcaca
     2701 accgccctca agctgacgcc gccccggccc tcgatgattg acgcgcgcaa tgccattctc
     2761 caggccagcc aggtgttagg cgcctcttcc tgcgatgtat ggactgtatt cgcccggcgt
     2821 ggccttggcg cctcagcggc attgaatcac atcgaggcgg gagagtcggc cgacacggcc
     2881 ctcagcgtat acgaagccta tgatctgcct gccgcttgcg gcggctcgcc tcccgcgccg
     2941 ggcgcaacta tcttctccga tgacatggaa accggcgcca tcggctggac ggcgaccggc
     3001 ctttggcacc ttacggcgcg gcgggcagcg agtggagcac actcctggtg gtacggtcag
     3061 gaatccaccg gtaattattc gacaggctcg gccaatagtg gttctctcac ctcgcccccg
     3121 atagctattc cagccggagc caaagtgatt ctggaatggg accaatattt ccagggcgag
     3181 ggcttcggcc gctgctatcc cttcgccggc cagggctgca atccctatgt caactatgat
     3241 tccggctggg tgaaggctag cgtgaacggc ggctcgactt ggttcacgct taccacgatt
     3301 gcccacaaca gcgacagtgc tgctttcgac cactacaagc tggatctctc cc
//
LOCUS       DASREK010000006        10010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_991271, whole genome shotgun sequence.
ACCESSION   DASREK010000006 DASREK010000000
VERSION     DASREK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991271"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VFA54_00285"
     CDS             <1..1375
                     /locus_tag="VFA54_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS49267.1"
                     /translation="AWLEGCYGVALGAAMTWLGGPKQMSRNAAGVCSTLAAVMFAATI
                     ACSFSRMGIAAVVCETVFFAVSQSQGRKRAAAALGAAACLGVSAVCAAGPKLLATRFA
                     AGSWRRELAARGAIWLDATRVICRYPLFGSGAGAFAFAFRRSHAYLGDYTIDHAHNEY
                     LEAAAELGVPVALLLGAGASAALVRRWHSARRARGPRRDIALGSLAGAFGLSLHSAVD
                     FPLRVPSILALFGLLLGMAFAADSETLSRKPPVGQGPDRLWAGLALLAALTLAAFHIW
                     AAPATAGGRRGLDRFNAELYYQAGRNAFVDGRLGEAKAACRRALQLCPYAAPVWQELA
                     LAAEAQGDVTGAVRWSDFALEIEPHTRRVEWVIGNLRLRLGDVNGALLLFHPLAAKTE
                     EMRRPVWDACWKAGIPAGRILESVVGRSRASQEEYLQYLEDRKRRTIELLRAAPVVWI
                     PASSSGH"
     gene            complement(1508..2755)
                     /locus_tag="VFA54_00290"
     CDS             complement(1508..2755)
                     /locus_tag="VFA54_00290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZS49268.1"
                     /translation="MTASPLALNSISREVPTARPHPASCGKPAIFGGEPLYAGRLPIA
                     RPAISGIGAGLLELCREILESGQLTNGQTVRKLEQAAADYLGVPECVAVSSCTAGLML
                     VERCLGLRGKVIVPSFTFFATAHSLLWNRLEPVFVDCDPETWNADPERVSRAIRPGVS
                     AILAVHVFGNPCPAFELECIARRAGLRLIFDSAHAFGAASAGSKAGAFGDAEVFSLSP
                     TKLLVAGEGGLITTRNRRLAAALRAARNYGDAGDYDCRQLGLNARLTEIQAAVGLASL
                     PSVERQVKLRNRLAAAYHACFQNEPGIAMQQIRPRDRSAFKDFSIVIEEARFGISRDF
                     LYSALEAENVQVRRYFDPPLHRQRLYRCFYRPDVDPLAITERISRNVLTLPIHAHMNE
                     YDAARIGARILAIRNWAARKRSG"
     gene            complement(2752..3825)
                     /locus_tag="VFA54_00295"
     CDS             complement(2752..3825)
                     /locus_tag="VFA54_00295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS49269.1"
                     /translation="MTEALRLIPASEETEWRRWLAPARHDFYHTARYHQFSEQSGEGQ
                     AWLAVYGSAQRYLAWPYLLRPIPGSRLRDVTSVYGYPGPLWVGPASDDCFLARARRQI
                     AELWRSQNAVSVFSRFHPLLENHRWMEGPLDSAVSGGIKPSGTTVSIDLTVPDAANWR
                     EYQPSLRNRINRARRLGLVTSADPAWNHLDQFALFYRATMVRNRALPFYFFPAEYFSR
                     LRAAMAPHAWLMLTRVEDRIAAAGIFIEYAGIVQNHFCVNNPDYLHLAPSKVLLDDVR
                     RWARLRGNRVFHLGGGRGGNCDSLFAFKAAFSQARHSFYTGRWVLDRDAYRRLCRMQP
                     CQPADFFPAYRAAAIAEEGVAST"
     gene            complement(3822..4694)
                     /locus_tag="VFA54_00300"
     CDS             complement(3822..4694)
                     /locus_tag="VFA54_00300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS49270.1"
                     /translation="MNPGEIIRPARVGEGERIAALIRLSLPPELRPLTIWDSPGAGNY
                     VESTLGGGVAGEDHCYYALQHGAHLEGVAVFRRLDTAAFLNHLYIAPHLRGRSLGTKL
                     LHQAARHYLHQAPLRRIALDVFPQCGVAETWYERLGFEESGRRNWWLLDSHAKVCGKN
                     VPAQILETCNGDPAWGFSRFQVLLPGGRCYEIGRLPGPYFRLTDRQAALDTELWEALS
                     RCDPSRKLLLIADSAALIRDFRRTDRDSSGRFQRLANHPMQRGGWKRAGVSRRLRCDT
                     ARLLRNLERGGVRA"
     gene            complement(4691..6439)
                     /locus_tag="VFA54_00305"
     CDS             complement(4691..6439)
                     /locus_tag="VFA54_00305"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZS49271.1"
                     /translation="MKLLYVATSDRFWPFLTGQAAYMKERGFDLHAASSPGPLLDAFA
                     ASQGAQPWPVPMRRGFSPLADLLALWRLWRVIRRIKPDVVHAHTPKAGLLSALAARAA
                     RAPAVVYTLHGLIWQTRKGWRRWLLICLDRLACRLAHQTLCVSESVRRQAIAFGLAPP
                     GRILVPANGSANGIDFERFDRSRFDVQGLGKLRRQYGLPDQALVMGFAGRLVPDKGIP
                     ELQSVWTRLRDGFPQLHLLLAGDTEEHDPLPAALLTKLRLDPRVHFTEWQTNMPEHYC
                     LMDLLVLPSRREGMPYAVLEAAAMGLPSAAFACDGVVDAVDHGVTGLLVSPGDTEALA
                     AACACLLADADLRLRLGRQARERAHRLYLQKPLWRHVYQVYCAALEAKRCPSRLWSKR
                     PADFAAALAALVLLAPLLGLIALAVWVNLGRPILYRQLRPGLGERLFPILKFRTMHEG
                     DGPDSERLTRFGSWLRRTSLDELPQLFNVIRGDMSFIGPRPLLERYLPYYTPRERLRH
                     TVRPGLTGWAQVHGRNDLTWRQRLEMDVWYVEHASLLLDLRIAAVSVWNVVACRGARE
                     DPGSLMLDLDVERLGI"
     gene            complement(6436..7689)
                     /locus_tag="VFA54_00310"
     CDS             complement(6436..7689)
                     /locus_tag="VFA54_00310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49272.1"
                     /translation="MRVLIASEAHFQRGDDGRVRAAGPEDYPFWSSYLGGARQVSVLG
                     RIGVMPPGATSGSIAEGEAVSFLPLDDYRGPWQYLRQYWRLRRQAQEAFSQCDAAILR
                     APGAVAYLAWHAVIRAGKPYALEVLADPREALAPGAARSLWRPLARRWSRWRLRRLCA
                     EAPVIHYVTREWLQRRYPAAKAKVFSYSDVLLRAPAGEKEMHERFERIRRTSAGERPW
                     HLGFIGSFEQLYKAPDVHLRAVRLCEVQGLNIRFSMMGEGRHRTNMEKLARRLGVAKS
                     VQFLGQINGADQVNHFLDQLDLFLMASRSEGLPRALIEAMARGCPAIGSSAGGIPELL
                     AAEDLVEPGSAGQLAAKIIQVLASPARLRTMARRNLEASQAFRPERLKPLRLRFLEEL
                     QMLAAASSSSARSGKRVPLSAELRA"
     gene            complement(7667..9097)
                     /gene="wzy"
                     /locus_tag="VFA54_00315"
     CDS             complement(7667..9097)
                     /gene="wzy"
                     /locus_tag="VFA54_00315"
                     /inference="COORDINATES: protein motif:HMM:NF025654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HZS49273.1"
                     /translation="MLAIVAGIGLRLALLAIDDDRTSALAAGALLLVCLFVCLRFLRL
                     WLLTPPMLYLCVFSLFHLGMAVPWALGIYAEKLPDWFLYNRLTPALALVTLATASYCS
                     GLIYSCRPNFEVLVEPAYANRFLFACGLFVYLAGGAMFFFGIQPLGGWRFFDAGYSET
                     YRLAAEFDPRFFGTSFTLVPIGLYLTAAAFPRRWMPVAVCITAIWVGGIFFLGFRGYA
                     LIPGLVVLCVLRQRGYRAPAWTNAALACIVLVAIPIARNVRDRGLHQRSMEPFSAGVH
                     PLAGIVEMGGSLRPLVHTLRYLESESWRWGKTYWQSLGTVWPNLAPRWEGGRYIALQD
                     LPPNHWLTLQAEPEMYRNYGGLGFSAVAEPYMNFGVPGVILYFWGLGSLLGWAAGRRS
                     RRPVRLAAWAMALGPLLWTTRNSFEIFFRPAVWGLIVVGAAWLASSLARSQTEASAGR
                     AEPGEKRGLTVGPGRRGDSSARAYSQ"
     gene            complement(9130..>10010)
                     /locus_tag="VFA54_00320"
     CDS             complement(9130..>10010)
                     /locus_tag="VFA54_00320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49274.1"
                     /translation="VRIAHSHNTQDGAEDCWLRNLYRAGAIRLLAKTATVRLACSADA
                     GRAFFAGADLPPNSGVRILRYGLSGHWDSGQGPRAAGARLRRELGIGNGEKVVGMVGR
                     FDRQKNHQFLLKVAAEMRRRDPRIRWLLVGGGPLLPLIEEEARRMGVQDCVRFCGVRE
                     DVRHLMFDVMDALILPSLHEGLPLALLEAQAAGLRALVSSRVTPEAAVVDGAVELLPL
                     EAGPAAWAGRVEACLELPKIPRASAWKQIAAAGFTLEQSLQDLLHIYRSSLGETLAQN
                     GRPVCSPYQMFASRNR"
BASE COUNT         2032 a         3097 c         3199 g         1682 t
ORIGIN      
        1 agcatggctc gagggttgtt acggtgtggc gctgggtgct gcgatgacgt ggcttggcgg
       61 gcccaagcaa atgtcgagga atgcggccgg cgtatgcagt acgctggcgg ctgtcatgtt
      121 tgcagccacc attgcctgct ccttctcgcg catgggaatc gccgccgtag tctgcgagac
      181 ggttttcttt gctgtttccc agagccaggg gagaaaacgc gccgcggcgg cgctgggagc
      241 cgcagcctgc ctcggcgtat ctgccgtgtg tgcggcaggg ccgaagttgc tggccacccg
      301 ctttgcagca ggatcctggc gccgggagct ggcggcgcga ggggcaatct ggctggacgc
      361 aacgcgggtg atatgccggt acccgctatt cggctccggg gcaggggcgt ttgcgtttgc
      421 attccggcgc tcgcatgcct atctcggcga ctacaccatc gatcatgccc acaacgaata
      481 cttggaggct gccgccgagc tcggagttcc tgtggctctg ctgctcggcg cgggggcctc
      541 ggcagcgctg gtcaggcggt ggcacagcgc gcggcgcgcg cgcggtcctc gcagggacat
      601 cgccctgggg agcctggctg gagctttcgg cctttcgttg cacagcgcag tcgattttcc
      661 gctgcgggtt ccctcgatcc ttgctctgtt tggcctgctg ctgggaatgg catttgcagc
      721 cgattccgag accctgagcc ggaaaccgcc ggtcgggcag ggaccggaca ggctctgggc
      781 ggggctggcg ctgctggcgg cattgactct ggcggcattt catatttggg cagctccggc
      841 gacagccggc gggcgccggg gcttggatcg cttcaatgca gagttgtatt atcaggccgg
      901 gcggaacgcc ttcgtcgacg gccgcctagg ggaggctaaa gccgcgtgcc ggcgcgcatt
      961 gcagctttgc ccttatgccg ctccggtatg gcaagagctg gctttggcgg cggaagcaca
     1021 gggtgacgta acaggggccg tgcgttggag cgatttcgcc ctggagatcg aaccgcatac
     1081 gcggcgcgtg gagtgggtga tcggcaatct gcggctgcgg ctgggagatg tgaacggcgc
     1141 gcttctgctg tttcatcccc tggcggccaa aactgaagaa atgcggcgcc ctgtgtggga
     1201 tgcctgctgg aaagcgggca ttcctgcggg ccgcattctg gaaagcgtag tcggcagatc
     1261 acgcgccagc caggaagagt acttgcaata cctggaagac cggaagcggc ggaccatcga
     1321 attgcttcga gccgcgccgg tcgtttggat tccagcctca tcctcggggc attagcgatt
     1381 tccttcagac ggagctgcgg cctccaagaa atctatggct acgactcctt gactccggcc
     1441 ctggagtagc cctgcttgta cgttctcgct ggctatctat ttcacgctta cttttcgacg
     1501 gcttggctca cccagagcgc ttgcgggcag cccaattccg gatcgcgagg atgcgggcgc
     1561 cgatgcgcgc agcgtcgtat tcgttcatgt gggcatgaat cggcagcgtc agaacattgc
     1621 gggagatgcg ctcggtaatt gccagcgggt ccacatcggg ccgatagaag cagcggtaga
     1681 gacgctggcg gtgcagagga ggatcgaaat agcgccggac ctgcacgttc tccgcctcga
     1741 gagccgagta aaggaaatcg cgagaaattc caaaccgggc ttcctcgatg actatggaaa
     1801 aatccttgaa agcggagcgg tctcgaggcc ggatttgctg catcgcaatg ccaggctcgt
     1861 tttggaagca ggcgtgatag gcggcggcaa ggcggttgcg gagtttgacc tgtcgttcca
     1921 cggagggaag gcttgccagg cccacagcgg cctgtatttc ggtcaggcgg gcgttgagtc
     1981 cgagctggcg gcaatcatag tcgccggcat cgccataatt gcgggctgcc cgcagcgcag
     2041 ccgccaggcg gcgatttctg gttgtgatca gcccaccttc accggcaacc aggagcttgg
     2101 tggggctgag gctgaagacc tcggcgtcgc caaacgcgcc tgctttgctt ccggcggaag
     2161 cggcgccgaa agcgtgggca gaatcgaaaa tcagccgcag ccctgctcgg cgcgcgatgc
     2221 actccagttc gaaagccggg caggggttgc caaacacgtg caccgccagt atggcggaaa
     2281 cgcccggacg gatcgccctg ctgacgcgct ccggatctgc attccaggtt tccggatcgc
     2341 agtccacgaa caccggttcg aggcgattcc acagcaagct gtgggcggtg gcgaagaaag
     2401 tgaagctcgg gacgatcact ttgccgcgca ggcccaagca gcgctccaca agcatcaggc
     2461 cggcggtgca gctcgaaacc gcgacgcact cgggcactcc gaggtaatcc gccgcggcct
     2521 gctccagctt gcggactgtc tggccgttcg tcagttgacc gctttcgagt atttcgcggc
     2581 acaactctaa taaccctgcg ccgatgccgg aaatggcggg acgggcgatc ggcaaacgcc
     2641 cggcataaag cggttctccg ccgaaaatag ccggcttgcc gcagctcgcc ggatgggggc
     2701 gggcggttgg gacttcccgc gagatgctgt tgagggccag gggcgaagcc gtcatgtgga
     2761 tgcgacaccc tcctctgcta tcgccgccgc acggtacgcc gggaagaaat ccgccggctg
     2821 gcaaggctgc atgcggcaga gccggcgata ggcgtctcga tcaagcaccc atcgtccggt
     2881 atagaaactg tggcgcgcct gcgaaaaggc agccttgaaa gcgaataggg aatcgcagtt
     2941 tccaccgcgc ccgccgccca ggtgaaaaac tcgattgccg cgtagccttg cccaccgccg
     3001 cacgtcgtcg agaaggacct tggaaggcgc cagatgcaga taatccgggt tattcacgca
     3061 gaagtggttt tgcacaatgc cggcgtactc gataaagatc cctgctgccg caatacggtc
     3121 ttctacgcgg gtgagcataa gccaggcatg cggcgccata gccgcccgga ggcgcgagaa
     3181 gtactcggcc gggaaaaaat aaaaaggcag cgcgcgattg cgcaccatgg ttgcgcggta
     3241 aaagagcgcg aactggtcca gatgattcca ggcgggatcg gcgctggtga caagacccag
     3301 tcgccgcgca cggttgatgc ggttgcggag gctgggttga tactcccgcc aattggcggc
     3361 gtctggaacg gtgaggtcaa tggagacggt ggttccggaa ggcttgatgc ctccggagac
     3421 cgcagaatcg aggggacctt ccatccagcg atggttttcc aacaaaggat ggaagcggct
     3481 gaatacgctg acggcatttt gagagcgcca caattccgca atctgcctgc gagcgcgggc
     3541 caagaagcaa tcgtcagaag caggcccaac ccacagaggc ccagggtagc cgtacaccga
     3601 cgtgacgtca cgcaagcggc tgcctggaat tggacgcagc aggtaaggcc atgcgagata
     3661 acgctgcgcg gagccataga cggcgagcca ggcctggcct tcgcccgatt gctcggaaaa
     3721 ctggtgatac ctcgccgtat ggtaaaagtc atgccgcgcc ggcgcgagcc agcgccgcca
     3781 ctccgtttcc tccgaggcag gaatgaggcg cagcgcttca gtcatgcccg cacccctccg
     3841 cgttccagat ttcgcagcaa acgggcagtg tcgcatcgga gtctccggct gacgccagcc
     3901 cgcttccatc cacctctttg catcggatga tttgccagcc gctgaaatct cccgctgcta
     3961 tcgcgatctg tccggcgaaa gtcccgaatc agcgcggcag aatctgcgat caagagcaat
     4021 ttacggctgg gatcgcagcg gctcagagct tcccacagtt ctgtatccag cgcagcctgc
     4081 ctgtcagtaa ggcggaagta cggcccgggc aggcgcccga tctcataaca acgcccgccc
     4141 ggcaagagaa cctggaatcg ggaaaaaccc caggccggat cgccgttgca agtttccaaa
     4201 atttgagcgg gaacattttt tccacagact ttcgcatggg aatccagcag ccaccagttg
     4261 cgcctgccgg attcttcaaa gccgagcctc tcataccagg tctcggcgac gccgcactgc
     4321 ggaaaaacat cgagcgcgat gcgccgcagg ggggcctggt ggaggtagtg tcgagccgct
     4381 tggtgcagca gctttgtccc gagcgagcgc ccgcgcaggt ggggagcaat gtacaggtga
     4441 ttcaagaacg ccgccgtatc gagcctgcgg aacacggcta ccccctcgag atgggcgcca
     4501 tgctgcaagg cgtagtaaca gtggtcttcc ccagcaaccc ctcctcccaa tgtggattcc
     4561 acatagttcc cggcgccggg agagtcccat atagtcaggg gccgcaactc cggcggaaga
     4621 gaaagccgga tgagggcggc gatgcgctct ccctcgccca cgcgggcggg gcggatgatt
     4681 tccccgggat tcatatgccc agcctttcga cgtccaaatc gagcatcaac gaccccggat
     4741 cttcccgcgc gccccggcag gctaccacat tccaaacgga gacggccgca atgcgcaggt
     4801 cgaggaggag agaggcgtgc tccacatacc aaacgtccat ttccaggcgc tggcgccatg
     4861 tgagatcatt tcggccgtga acttgcgccc atcctgtcaa accaggacgg acggtgtgcc
     4921 gaagccgctc tcgcggcgtg taatagggga gataccgctc taacagcggc cgcggtccga
     4981 tgaagctcat atcgccgcgg atgacgttga atagctgagg gagctcgtcg aggctggtgc
     5041 gccgcagcca gctcccgaag cgggtcagcc tctccgagtc gggaccgtct ccctcatgca
     5101 tggttcggaa cttgagaatc gggaacaggc gctcgccaag accgggacga agctggcggt
     5161 agagaatcgg cctgccgagg ttgacccaaa cggccagcgc gatgaggccg agcaggggag
     5221 cgaggagcac aagcgcggcc agcgccgcgg caaaatcggc gggacgtttc gaccaaagcc
     5281 gggatgggca gcgcttcgcc tcgagcgctg cacagtagac ctgatataca tgtcgccaca
     5341 acggcttctg gaggtagagc ctgtgcgccc gttctcgggc ctgccggccc aagcgcagcc
     5401 tcaggtcagc atccgccaac aagcacgcgc aggccgccgc gagcgcctct gtatcgccgg
     5461 gggaaacgag caggccggtt accccgtggt caacagcgtc cacgacgccg tcacaggcaa
     5521 aggcagccga agggagtccc atggctgcgg cttcgagcac ggcatagggc atgccttcgc
     5581 ggcggctggg caataccagc aagtccatca ggcaatagtg ctcgggcatg tttgtctgcc
     5641 attccgtgaa gtgaacccgc gggtcgagcc ggagcttggt gagaagcgcc gccggcagcg
     5701 gatcgtgctc ttcagtatca ccggccagca gcaagtgcaa ctggggaaat ccgtctcgca
     5761 ggcgtgtcca gaccgattgc aattcgggaa ttcccttgtc cggcaccagc cggccggcaa
     5821 atcccatcac caaggcctga tctggcaggc catattgccg ccggagtttc cccaggcctt
     5881 gcacatcgaa gcggctgcgg tcaaagcgct cgaaatcaat tccattggcg ctgccgtttg
     5941 ccggcacaag aattcggcca gggggcgcca ggccgaatgc gattgcctgc ctgcggaccg
     6001 attcgctcac gcaaagcgtc tggtgcgcga ggcggcaggc aaggcggtcg aggcagatca
     6061 gcagccatct ccgccatcct ttgcgtgttt gccaaatcag gccatgcagc gtgtagacga
     6121 ccgccggcgc tcgcgcggcc cgcgcggcca gggctgacag cagaccggcc tttggcgtat
     6181 gggcatgcac gacgtcgggc ttgatgcgcc gtatcacgcg ccacaatcgc catagagcaa
     6241 gtaaatcagc cagcggcgag aacccgcgcc gcatcggaac cggccacggc tgcgcccctt
     6301 gggaagcagc aaaggcgtcg agcagaggtc ccggggagct ggcggcgtgg agatcgaagc
     6361 cgcgctcttt catgtacgcc gcctgccccg ttaaaaaggg ccaaaagcga tcgctggtcg
     6421 cgacatacag aagtttcatg ctcgtaactc cgccgaaagg ggcactcgct tcccggagcg
     6481 cgcggatgac gaggaagccg ccgcaagcat ttgaagttct tccagaaaac gcaggcgaag
     6541 aggcttcaag cgctccggcc tgaaggcttg cgacgcttct agatttcgcc tggccattgt
     6601 cctcaggcga gccggagaag caaggacctg aataattttg gcggctagct gtccggcgct
     6661 gcctggctct accagatctt ccgccgccag caattcagga atgcccccgg ctgaagagcc
     6721 gatcgccgga cagcccctcg ccatcgcttc aatcagcgcc cgcggaaggc cctcagagcg
     6781 agaagccatc agaaacagat cgagctggtc cagaaaatga ttcacttggt ccgctccgtt
     6841 gatttgtcca aggaattgaa cgctttttgc gacccccaag cggcgagcga gcttttccat
     6901 gtttgtccgg tggcggcctt ctcccatcat ggaaaatcga atgttgagac cttgaacttc
     6961 acagaggcgt accgcgcgca agtgaacgtc gggcgctttg taaagctgct cgaacgagcc
     7021 gatgaaacca agatgccagg gccgctcgcc cgctgatgtc cggcgaattc gctcgaaacg
     7081 ctcgtgcatt tctttttcac cggccggtgc gcggagcagg acgtcagagt acgagaatac
     7141 tttggctttc gccgccggat atcgccgctg gagccactcg cgcgtgacat agtggatcac
     7201 cggagcttca gcgcacaagc ggcgcagcct ccaccttgac caccggcgag ccagcggccg
     7261 ccacaagctg cgggcagctc ccggcgcgag cgcctccctg ggatccgcca ggacctcgag
     7321 ggcatagggt tttccggcgc ggataactgc atgccatgcc agataggcca ccgcgcccgg
     7381 ggcgcgcaaa atagcggcat cgcactgtga gaaggcctcc tgcgcctgcc gccgcaagcg
     7441 ccaatattgg cggagatatt gccaagggcc cctgtaatca tcgagcggca ggaacgaaac
     7501 tgcttcaccc tcggctatgc tccctgaggt tgccccgggc ggcatgaccc cgatccgtcc
     7561 gagcacgctg acctgccgag cccctccgag ataggaagac cagaacggat agtcttccgg
     7621 accggcggca cgaacgcggc catcatctcc tctctggaaa tgagcttcac tggctataag
     7681 cacgcgcact actgtctcct ctgcggcccg gaccaacagt gaggccgcgc ttttccccag
     7741 gctccgcgcg cccggctgac gcctcggtct gtgagcgcgc cagagacgaa gcgagccatg
     7801 cagcgcccac tacaatcagg ccccacaccg ctggccggaa aaaaatttca aagctgtttc
     7861 gcgtggtcca gaggagcggg cccagcgcca tcgcccaagc ggccaacctg accggccgcc
     7921 gggagcggcg gccggcggcc catcccagca acgagccgag gccccaaaag tagagaatta
     7981 caccgggaac gccgaagttc atgtaaggct cggccacggc ggaaaaaccc aagccgccgt
     8041 agttgcgata catctcgggt tcggcctgca aggtaagcca atggttgggc gggagatctt
     8101 gaagcgcgat gtaccggccg ccttcccagc gaggcgcaag attgggccac acagttccca
     8161 gcgactgcca ataggtcttt ccccatcgcc aggattcgct ctccagatag cggagggtgt
     8221 gaaccagagg acgcaggctg ccgcccatct cgacaattcc ggcgagcgga tgcactccgg
     8281 cggagaacgg ctccatggat cgctggtgca agccccggtc ccgaacgttg cgggctattg
     8341 gaatcgcaac gagcacgata cacgcgaggg cggcgttcgt ccacgcgggc gcgcggtagc
     8401 ccctctggcg gaggacacag agcacgacca atcccgggat gagcgcgtag ccgcgaaatc
     8461 ccaggaaaaa gatgccgccc acccatattg cagtgataca tactgccacg ggcatccacc
     8521 ggcgtggaaa cgcggctgcc gtcagataga gaccaatggg gaccagggtg aagcttgtgc
     8581 cgaaaaaacg cgggtcaaat tcggcggcga gccggtatgt ttccgaataa cctgcgtcaa
     8641 agaaccgcca tccgccgagc ggttgaatcc caaagaaaaa catggcgccg ccggccagat
     8701 aaacaaatag cccgcacgcg aacaagaatc gattcgcgta agcgggctca acgaggactt
     8761 caaagttcgg gcggcaggag taaatcaaac ccgagcaata ggaggcggtc gccagagtta
     8821 cgagagccag agccggcgtc agcctgttat atagaaacca atcaggcagc ttttcagcgt
     8881 agatgcccag ggcccagggc acagccatcc ccaagtgaaa cagggaaaaa acgcagagat
     8941 agagcatagg cggggtcaag agccacagcc gcaaaaacct caggcacacg aaaaggcaaa
     9001 cgaggagaag cgctccggcc gccagcgccg atgtccgatc gtcgtcgatg gcaagcagcg
     9061 ccagccgcag gccgatgccg gcaacgatgg ctagcattga caggagccgc cggaagcggt
     9121 gcagggaagt catcggttcc ggctcgcaaa catctggtac ggactacaaa ccggccgccc
     9181 gttctgggct aaggtctcgc ccagggacga acgatagata tgaagtaaat cctgaagaga
     9241 ctgctcgagg gtgaatcccg ccgccgcaat ctgcttccac gccgaagcac ggggtatttt
     9301 cggcagttcc aggcacgcct caacgcggcc ggcccaggcc gcagggccgg cttccagggg
     9361 cagcaactcc acagcgccgt ctaccacggc ggcttccggc gtaacccgcg atgagaccag
     9421 cgcgcgcagg ccggccgcct gcgcttccag cagcgccagc ggcagacctt catggagcga
     9481 gggaagaatc aaggcgtcca tgacatcgaa catcaggtgg cggacgtcct cgcggacccc
     9541 gcagaatcgt acgcaatctt gcacgcccat gcgccgggct tcttcttcga tcagcgggag
     9601 caagggcccg ccgccgacca gcagccatcg gattctcggg tccctgcgcc gcatctcggc
     9661 ggccaccttc agcaggaact ggtgattctt ttgccggtcg aaacgaccga ccattccgac
     9721 tactttttcc ccattgccga tgccgagttc ccggcgcagg cgcgcgcccg ccgcgcgagg
     9781 cccttggccg gaatcccagt ggccgcttaa gccgtagcga agaatcctga cgcctgagtt
     9841 gggcggcaaa tccgcaccgg cgaagaaagc cctgcccgcg tccgccgagc aagccaagcg
     9901 aaccgtggcg gttttggcca gcaggcgaat tgcgccggcg cggtagagat ttcggagcca
     9961 gcagtcttcg gcgccgtcct gtgtgttgtg gctatgcgcg atgcggaccg
//
LOCUS       DASREK010000007        35184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_332031, whole genome shotgun sequence.
ACCESSION   DASREK010000007 DASREK010000000
VERSION     DASREK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 35184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35184
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332031"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /gene="ligD"
                     /locus_tag="VFA54_00325"
     CDS             complement(<1..1437)
                     /gene="ligD"
                     /locus_tag="VFA54_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZS49275.1"
                     /translation="MSLEDYTRKRSFNKTPEPLPSPPRPESAVRRFYVQRHHARRLHY
                     DFRLEIGGALKSWAVPKGPTLDPSEKRLAVMVEDHPLEYGDFEGNIPEGNYGAGSVMV
                     WDRGFYELVGDAAAEQQIERGDLKFRLHGEKLRGEFALVRMKNRGKGNEWLLLKKKDS
                     AAQPGWDPEQHPRSALTGRTQEEIARGMPPFVSRSPTARNRKNIAMPSEAVRGPMPAS
                     IVPMQAFSAGEPPEGPDWLYEVKWDGVRAICFLQDQKIRLVSRNGNPADAQYPELSVV
                     PHYVAAREAILDGEIAVLDAQGRPRFELIQPRIMVSDPNAIAQLSRTRPVVYFIFDLL
                     YLDGYDLRQAALLERKRILASILEPCPVLRYSEHFAGSGREILEASRRQGLEGIMAKR
                     ADSRYEPRRSRDWLKIKIVSQQEFVICGYTHGDRGGYFGALVLGVYDQGKLVWAGNVG
                     TGFDQPMLERVYAMLQPLVSPRCPFPETP"
     gene            1625..2476
                     /locus_tag="VFA54_00330"
     CDS             1625..2476
                     /locus_tag="VFA54_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZS49276.1"
                     /translation="MATTVWKGHLTFGLVSLPVKLYSAARSESVSFNQLHKTDNSRVK
                     QVIYCQAEDKPIPRSEIVKGFEYEKDKYVVIEDEEIKKVAPKTAKTMEILEFVKASEV
                     DPLYLESSYYLAPDEAGERPYALLFEALRRTGYLGLAKVTMHNREYIVILRPGDRGIL
                     LHTMYYADEIRRVEEFRTDTSVIKEKELELATTLIESLKAEFHPEKYRDTYRENLKAL
                     IKAKVEGQEIVEAPAPAMAPVIDIMEALKKSLDLAKKPVQSTTEAARPAAVETRTKGR
                     KKSAAGQ"
     gene            complement(2494..2922)
                     /locus_tag="VFA54_00335"
     CDS             complement(2494..2922)
                     /locus_tag="VFA54_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZS49277.1"
                     /translation="MAAQLATKKALTLEVAKQMAAYAEAEARKNQWNVVVAIVDDGAN
                     LIYLQRMDDTQIGSIDVAQQKAKSAIRFKRPTKVLEDVVAGGRLGMMQLAGSIPVEGG
                     LPIAVDGQIIGAIGVSGVQSNQDGQVAKAGLDALPKILGA"
     gene            complement(2986..3741)
                     /locus_tag="VFA54_00340"
     CDS             complement(2986..3741)
                     /locus_tag="VFA54_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZS49278.1"
                     /translation="MAKPIANAAAEGVNPMPQLFAGTSGFAYPQWKPAFYPADLPEKR
                     FLEYYSRRLNAVEINYTFRRMPAASTLENWVYLTPPGFLFAIKGHQRITHILRLRDAA
                     DATGIFLRSIDPLRATRRLGPILFQLPPAFPCDVKLLGEFLAALPDDLRYAFEFRHAS
                     WLIDPVYRLLEQRNIALCLAESDKFEAPEVITARFVYSRLRKEEYSPEDRKAIAGKAR
                     KLLGEGKDVFLFFKHEEKPDGALYAEEVLQAVH"
     gene            complement(3766..3860)
                     /locus_tag="VFA54_00345"
     tRNA            complement(3766..3860)
                     /locus_tag="VFA54_00345"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3824..3826),aa:Sec,seq:tca)
     gene            complement(4018..5304)
                     /locus_tag="VFA54_00350"
     CDS             complement(4018..5304)
                     /locus_tag="VFA54_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZS49279.1"
                     /translation="MPLIESETRNIEKAQLPNGLSVISEVMPHVRSVSIGIWIKTGSR
                     HEAPDQNGISHFIEHMVFKGTTTRSAEEIARSIDSVGGHLDAFTAKEMISFNTKVLDE
                     HLPLAFDVLSDLVLHPVFQEDDILKEQGVVLEELKMDEDNPDLLVHEIFSGNFWKDHS
                     LGKPIIGSKKTIKSFHRESLIEYYKRVYVPSNILVTAAGRLDHDDLMRLVERAFGDLD
                     GNPPPPDDRTPVTHAIISLRDKPALEQVHLCLGVPSYPLADGRRYACYVLNTLLGGGM
                     SSRLFQNIREKQGLAYSVYSELNPYRDTGCLSVYAGTSIEAAERVVESILKEFREIKE
                     GRITPEELRRAKDHLKGSLMLSLESTSSRMANLARQEMYFPRFFSLDELIEAIEQVTA
                     AEVQQIACESFNQKQIALTVLGNLNGFKLGREKLVC"
     gene            5551..6627
                     /gene="rlmN"
                     /locus_tag="VFA54_00355"
     CDS             5551..6627
                     /gene="rlmN"
                     /locus_tag="VFA54_00355"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZS49280.1"
                     /translation="MPLPLVGLDENDLLQIVTARGEAPFRARQLYGGIYRSHAAVLED
                     VRGLPAVFRRALEQEFEVGLPAIAREFRSSDGAIRYLLRLGDGQTIETVLMPEEDRDT
                     ICISTQVGCPVDCRFCFTAMLGLTRNLSAGEIVGQVLVAVRQNGLLARDTRRRLNIVM
                     MGMGEPLLNLPNVVKATRLLTDPQGLNLSPRRITVSTSGITPKLAELGREPVRPRLAI
                     SLNASTQEMREKLMPITRKYKLAELLEACRAYPLRPWERLSFEYVLLAGVNDSDQDAR
                     RVVRLLSNLKCMVNLIAFNPGPGIDFKTPEPERVLAFQALIQRSLPCFIRKPRGRDIY
                     AACGQLKRMGQEAADQGPNPILSL"
     gene            complement(6617..7501)
                     /locus_tag="VFA54_00360"
     CDS             complement(6617..7501)
                     /locus_tag="VFA54_00360"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZS49281.1"
                     /translation="MKLQGIFPPIATPFDHHGDLYMAKIRHNVEKWNRTQLAGYVVCG
                     STGESVMLTCEEKVRLWDGVREAAAPEKLLIAGTGAESVRETVQLTNMAASIGYKAAM
                     VRTPHYYKNLISNGAAQALYFRAVADQAKIPIIIYNWPQVTGIDIPAQTVIELAEHPN
                     IIAVKESSGNMEKTMQMVRDAPEGFQVLVGSAPTLWLSLMAGAAGAILAFANAAPYAC
                     ISIYEAVRTREYAAAADLQRRFTPAAVAVTTKYGIPGLKYAMDLNGYYGGPPRLPLIP
                     VGPEAKREIEEKFRDIKG"
     gene            complement(7666..9300)
                     /locus_tag="VFA54_00365"
     CDS             complement(7666..9300)
                     /locus_tag="VFA54_00365"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS49282.1"
                     /translation="MNLGIAILAALLLQAPIPRAGPPAPAEGSSASASESQGEISGRF
                     VCAQTGEPLKGVSVEILAEVHGHPFQQGILSAADGSFSFQHLPAGNYQIRAQKSGYRP
                     VAGSPTSFPLEPGQIRRGLEIKLNRAAIITGRVTDAEGEPVVRASVSAYRFVWRAGRR
                     SLAPADSVATDDRGLFRFYRLPAGRYIIGVRFPAEDQAAGEGDLRLAETFYPNGSSAS
                     EAVVLETRWGQELSEINMVLRRQRTFSISGRVFDSESGGPCRGCAVSVGRAGDQIPPM
                     HFGVAADGGYRARGLTPGTYFITAFKTTAGRHVAVESRQVQITSEDLRDVYVAVGPGR
                     SVTGRVVFDPPDTVIENARMLVALPPESEPELRSSFRVLPDFSFRADGLSVGMHRVQL
                     DGIPNNGYIKAVRLSGRDLPAPELEIPGDGSVSRFEIVIGFDSATLAGQVKLPDPSGE
                     HRITAAVIALFPQDAQASPFLAERRGFTQPGGAFTIPGIPPGSYTVYAFPASGNFEWE
                     DPGVRRAAESYGKSVDLRPGQKQTIDLPLAPEIMAP"
     gene            complement(9297..10997)
                     /locus_tag="VFA54_00370"
     CDS             complement(9297..10997)
                     /locus_tag="VFA54_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS49283.1"
                     /translation="MLRLAFLLLPAAGVWAATISGTVWLHDGKIPKPISGVHVVARAA
                     DGRDLLQTAETDSQGRYLLQNLPEKRVALSAARPGYFGRAVAGAEPELLLDLSSDNTL
                     ERADFELFPGGVITGRITDALGEPLENIEVRCWRVIHADGRHQALAPGAVTDDRGVYR
                     IFGLEPGSYILLARPQFRRKPPLAAAYFPGTAQESNAKEIEVAPGAEVTGIDLTLGDQ
                     PAYTISGKIAGADPEQIRGANILAIPISGESAAAARSANPDELNWRMGIGMTNGGVAT
                     VDEKGDFFLSGLPAGEYVLTVSARPGPVPAGMRAQIAAIVRQAVDVRSNMTGVLLHAA
                     RVARISGRVSFPKGPPRRSRPEAIVLELADQSPVVLGARSREAAARSPDYKFEFPDLL
                     PGSYTLRIVSPSAVYMMVEGEPSEAKEITVPEGAALDVEVETGFGTASLSGIVKEPGS
                     GAPLPEARVAMAKAPPESRTGGSPIRFRTAQADQRGRWSIPDLLPGAYLICAWSSASV
                     ESLYAPETWQAAGTAVRKIVVDPNTQVEVELTSSEVPAAGRRPQTPSSGASIRQPRRP
                     "
     gene            11052..11378
                     /locus_tag="VFA54_00375"
     CDS             11052..11378
                     /locus_tag="VFA54_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49284.1"
                     /translation="MKQNRGAAIKLPKIPKKEHTRYAGMAYRPGGLSVPLKVVEEAVE
                     TVIRRRLEKEWGRPVRRSELRRDPSLPPLTEHPLLRKLARAKNRKTAPTAPKGKTGKR
                     PGIASH"
     gene            11381..11992
                     /locus_tag="VFA54_00380"
     CDS             11381..11992
                     /locus_tag="VFA54_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49285.1"
                     /translation="MSEYDKSIFLNCPFDSAYKPIWDAIVFTLTQADLRPRCALEVDD
                     AAENRLEKIYRIVAECRYAIHDISRTELDPVHALPRFNMPLELGIFLGAKKFGSRNHS
                     RKACLILARELYEHQKFLSDLSGQDVHAHHNDPRLAVIEVRNWLRTAAPRLDLRGGEQ
                     TWRRYQVFREELPQICGAMGLTVEGMTFVDLLAAVSLWLRRNP"
     gene            complement(12074..12742)
                     /locus_tag="VFA54_00385"
     CDS             complement(12074..12742)
                     /locus_tag="VFA54_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49286.1"
                     /translation="MLLRNSFPGVHFPAYIGFAQDPGGALIERSTKIRRRYDKKIGAM
                     LQRCCLLILTCVAASGFQGGGGSPSDASRRADVYAIYSLLLANAPLSHPGDNQVYLVA
                     GTTVPGVPREPCVRPPDEYAAGFAEIMADYGKRKNVAVILQPRFQLDKPYHLLNAGEV
                     KEFVELHVLRPGPRPKPRELFEKSKDLFYLTNVFLQPKPHTSSYSHPLLLRTIVRQSG
                     LEGF"
     gene            12741..13535
                     /locus_tag="VFA54_00390"
     CDS             12741..13535
                     /locus_tag="VFA54_00390"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS49287.1"
                     /translation="MIRICLPVLGFALAVQLAAQPAIQLNDGDSHGDPPYLLEPGWKP
                     LLNGRDLSGWHSQDGKPLQWFTTTAIRWERLLGPTRLFAIPAPGGTILNSAQGRTSNL
                     VTDQKFGDVELYLEFMLAKGSNSGVYLHGLYEVQIFDSYGSWEPMTSSDCGGIYHRWI
                     DERGVGGSAPSRNASRRPGEWQSFQIWFRAPRFDADGRKVEDARFLRVLHNGISVQKD
                     VAVDGPTRAAMDHPEAAENPLMLQGDHGPVAFRNIYIRPLRPIIER"
     gene            13701..14924
                     /locus_tag="VFA54_00395"
     CDS             13701..14924
                     /locus_tag="VFA54_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZS49288.1"
                     /translation="MFNRIKFGIVVGSTFLTALLVIGAVMGDSSSADGAYRQLSVYTE
                     VLSRIKSDYVEEPDIKQVTRGALQGLVESLDPNSSYLTAEQYKEYEKRKSSQQAGVGL
                     IISKRFGYVLVLAALPGSPAAKAGLSVGDLLEAVDKKATRDLALPLIETMLNGPAGST
                     VTLVVRRARHAEEPEELTLTRMAVPTPPVVHKLLTDKVGYLDVKALTPGKAAEAGRAI
                     RELMSQGVERLVLDLRNDAMGDNQEGLKLANLFIDNGLLGYLEGQKYPRKDFMADPRQ
                     AICKLPVAVITNRATAGAAELAAAAIVDRNRGQIVGEKTFGLAAEQKTIPMEDGAAII
                     LSVAKYHRPGGKAIQDGGVNPTLQVSETDNEAAVDDDNAPTPSPSPSPKSPEEDELLK
                     KAIEVVKGAAARAAA"
     gene            15047..15676
                     /gene="pgsA"
                     /locus_tag="VFA54_00400"
     CDS             15047..15676
                     /gene="pgsA"
                     /locus_tag="VFA54_00400"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HZS49289.1"
                     /translation="MTLPNALTVLRIFFVPLLLAVLLRKEIYLETPWITLSKEWLALP
                     IFLAAALTDLLDGIIARRRGQISTLGTLLDPVADKLLISAAFISLVELGVVPAWMVVV
                     IVGREFAVSGLRSIAAAEGYTIEASELGKTKMVMQVIAVSLLLAGLHWPFVLRLGIVA
                     VWLVVLFAIVSAVDYFRKFWRRVDIRIKVRRRRERLLLLRKQRKDVAAH"
     gene            15660..16244
                     /gene="amrA"
                     /locus_tag="VFA54_00405"
     CDS             15660..16244
                     /gene="amrA"
                     /locus_tag="VFA54_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZS49290.1"
                     /translation="MLPLTSVARNWLLVSARCAVEAAARGEIPAISQIPPEIPASDRP
                     WLEQPHPVFVSLHLEGRLRGCVGHVGLEIPLRCLVPEMAYAAACEDTRFQPVTAEEAL
                     RIDIEIFILSPFFSIRPEEIIPGTHGLIVRQGQRRGILLPCVAAEHNWDAVRFLCETC
                     RKAGLAPDAWKHGAVIEAFTAEVIKQAALAGASP"
     gene            complement(16270..16956)
                     /locus_tag="VFA54_00410"
     CDS             complement(16270..16956)
                     /locus_tag="VFA54_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZS49291.1"
                     /translation="MREITARHGIVLIFDEVITGFRCSPGGAQAHYNVTPDLTTLAKI
                     VAGGYPGAVLAGRAEILEVMEYHHSEEGIQPPAVPHQGTFNGSPLSAAAGIATLRLLR
                     DGEMIAQANRTAAAIRTEMNRALARLGASWCVYGDFSGFHMYLNSGSAPVSPQDVHAG
                     KVPWTKLKGGAPAELLQKIRAGFLCAGVDVIGWPGGLVSGVHNGEDIDRTVSAFEKLL
                     RLLAEEGDLG"
     gene            17144..17404
                     /locus_tag="VFA54_00415"
     CDS             17144..17404
                     /locus_tag="VFA54_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49292.1"
                     /translation="MIVPAMKVAGEAHQHGLPGISPSNAQSQQRRFGARGCKAHALRA
                     RDHLPNQLAPFDLQLMGSAVVSPPRKLRGYCLYDRWMIVAEH"
     gene            complement(17647..19590)
                     /locus_tag="VFA54_00420"
     CDS             complement(17647..19590)
                     /locus_tag="VFA54_00420"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZS49293.1"
                     /translation="MLHLSMAPQDSAKGPLYCTIAASLLFLCLNLLGAQQSVSVQAPV
                     SGEVAAGAVIFRAHCAPCHGIRGEGGRGPDLRKTSFRHGSTDADLLRIISKGIPGAEM
                     PGIFLPESEIRRIIQFIRSLPPVLVRGKAALPASPPVTYERLLRADREPENWLMYSGN
                     YNGNRFSSLDQITTENVARLRFKWAYQMKTVEKVEATPLVVDSVMYLTEPPNAVSALD
                     AGTGRPLWTYRRSLPEKINVCCGQVNRGLAILGDRLFVGTLDAHLLALDRNTGSVIWD
                     VAVADYRMGYSITAAPLAVKNTIVTGIAGGEYGIRGFVDGYDAGTGKHLWRFNAIPGP
                     GEPGHDGWAGESWKTGGGPSWLTGSFDPELNTIYWGIGNPAPDWNGAVRPGDNSYSNC
                     VVALDADTGKLKWYFQFTPHDVHDWDAVQIPVLVDALWQGRPRKLLYWANRNAFFYVL
                     DRESGEFLLAKPFVRQTWAGGINKQGRPMPYPGSDPTARGTLVYPGVQGATNWYSPSY
                     SPLTRLFYLSVWDYATRYQLGDAVYSPGNRYAGSVPVDIPEDPGAGSIRAINPQTGAV
                     QWEHKLPSKPQSGVLSTRGGLVFGGINEGEFFALDAQTGREVWHANTGGMIAAGPITY
                     LERGRQLVSVAAGSSIFTFGLDE"
     gene            19662..20177
                     /locus_tag="VFA54_00425"
     CDS             19662..20177
                     /locus_tag="VFA54_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49294.1"
                     /translation="MRRAVAVAFFLLNLASCSRRQNTQDPPLVEEEHPESMLSTLHVA
                     DPKAVPQLLKGWHAVEQGSWRWTQKTFAVALKAPSPGKPAKLQLKFVIPPPLLSRLHS
                     ITLNASIDGVSLGPETYNQAGDQTYTRTIAPRETSGNLIRVDFQLDKAVPPDNRDKRE
                     LGVVVTSVGLE"
     gene            20178..22055
                     /locus_tag="VFA54_00430"
     CDS             20178..22055
                     /locus_tag="VFA54_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49295.1"
                     /translation="MQPAASTIETPRRHAALPQAALWCLPLLLCLAFFWRSLFSWFQQ
                     DDFAWLYLRLQLHSASGLWRLLMEPRAEGTIRPLSERLFFIVFRSLFGLNAFPYHLFV
                     LLTQFANLALLLALARRLTASLGAASLTAALWAVNVSLLTPMMWASAYNQILCAFFFL
                     SSLLLFLKHVETGRQRFYVAQGCTFLVGFGAHESMAVYPLILLGYCALLNRRHAWKAA
                     PLFIPSALYTAAHLWLIPHSASGPYALHLDLSVIPTLKAYWSWALGPPLFAASLHWPG
                     RASTALWVPLTLSLGAAMMRAGRPKRRAALFGLAWFFLTLAPVLPLRDHRMPYYLVIP
                     AIGLALAAGSVAGSAPRWAAAAWLMVYFGCSSAFLIREAPLVCERSRAAKRFLEALRQ
                     ARASHPDKTILLTGVSSELFYSVIYDHGPLAVRLSEVYLAPDSEGIAPQPGLDAVSNF
                     ELPARITLAALRAGDIIVYDASGSNLKNVTSLYAVNAPERLKDAPPRRIDLGEKLIAA
                     QLGPGWYEHQVNHRWMGERAEVRLAAPRSAGERLHIRAIYPDHYHVGTIYLTVLVNGI
                     PAGRAAICDSRSSELTFSLPASVTGLSEMTVTLEAGPTFRPPGDTRNLSLAFGVIELL
                     E"
     gene            22120..23079
                     /locus_tag="VFA54_00435"
     CDS             22120..23079
                     /locus_tag="VFA54_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZS49296.1"
                     /translation="MSKAFDVVGVGLNATDTLLLVPHFPEYAGKAPIVEELMSPGGQV
                     ASAIVTCQRLGLRTKYIGTVGDDERGAIQMQSLLQEHINVDHVQIRQGCPNQSAYIIV
                     DQRTGERTVFWRRDESLRIDPSQIAAEQITCARLLHIDGHDTAAVEHAARIAHEAGMP
                     VTVDVDTIYHGFDRVLRYVDYLIASSEFPVQWTNQRDPFRALELIQQEYGMKVAAMTL
                     GAYGSLAYSEGRFFYSPAFVVNCVDTTGAGDVFHGAFCYAVLENLPLDEALEFANAMA
                     ALNCTVLGARGGILGSAEARRLMARAERRSNKDFEIRASIPVG"
     gene            23094..23339
                     /locus_tag="VFA54_00440"
     CDS             23094..23339
                     /locus_tag="VFA54_00440"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZS49297.1"
                     /translation="MIPRVTLYTRSGCHLCDEAKEQIRRARRRTPFDLEEIDIDSDPG
                     LRALYDEEVPVVAINGKKAFKYRVKEKELLTKLRSRS"
     gene            23428..24138
                     /gene="bshB1"
                     /locus_tag="VFA54_00445"
     CDS             23428..24138
                     /gene="bshB1"
                     /locus_tag="VFA54_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HZS49298.1"
                     /translation="MALDLLAIAAHRDDIEQTCGGTLIRMAEKGYRTGALDLTAGEMG
                     TRGDAETRAREAAEAAREMLLSWRGNAGLPDAKLELSLPAKLAVAAKIRELRPRAVIL
                     PYWEGRHPDHYRTSTLCYEACFLAGLKKLDLAGEAFRPAKILYASLYANVRPSFVVDI
                     TGQFERRMRALLCYRSQFEDQPAGADLFPARDELIERIRSVARYYGMLIGVKYGEPFV
                     VKEMISVDDVVKMPVRSI"
     gene            complement(24228..26168)
                     /locus_tag="VFA54_00450"
     CDS             complement(24228..26168)
                     /locus_tag="VFA54_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZS49299.1"
                     /translation="MFYLFRSRKRVMRIFLGSILGIVCITMVITLIPGIYGNAGTSTN
                     DTVLAQVGKEEITQAEAQAQLQDTIRSQRLPSQAYNFLAPQVVRNMIDDAVLLQEAER
                     LGLNVTEEELANVLRASWPQLFPDGKFVGKEQYAQYVYQLFEKSVPEFEALARKDLAL
                     SKLRRLVTDGVVVTPAEVEQEFKRRNEKVKIDYALVTPASVMSSVSSTPAEIEEYFKK
                     NRNMYMVPEKRSFQYVVIDDAAVASRVKVTPEELQRYYNENKERFRVQDRVHVTHILF
                     KTTDKKPEEVKQIEAKAQDVLKQVRAGKDFAALAKENSEDTASAAKGGDIGWITRGQT
                     VPEFEQKAFSMKPGEVSDLVKTQYGFHIIKVLEKETARLKPFQEVEPTIREEFMRDRS
                     DAEKSALMDRLRTAATRHGQNLQEAAREVGLSVNTATMLDRNSAIPQLGNETGVMESL
                     FSAAKGSLVGPIQMTGNKAIMAVITEVAPARQAEFSEVAERVKNDCNTAKARDLVKAR
                     ADKLAERAKALGDLRKAAREFGVEVKSSDLFTRDGSIPGLGGASTVSDAFNVPPHSVV
                     GPVRAGADYAVYQVTERVGADMSLFASESKVLHDTMTGTRQNEAFDIFKDELRKRMEK
                     EGKVKVYQSRVDRFVASNQRNS"
     gene            complement(26354..27613)
                     /gene="oxlT"
                     /locus_tag="VFA54_00455"
     CDS             complement(26354..27613)
                     /gene="oxlT"
                     /locus_tag="VFA54_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HZS49300.1"
                     /translation="MKNRWVQLVGSVVAMVMIANLQYAWTLFVQPIQAGTGWKLSDIQ
                     WGFSLFLVCETWVMPLEGWLIDRMGPRIFISIAAVLCGVGWTTLGFVRQLWQLYLFYA
                     IAGVGAAFVYSGSIAAALKWFPDKRGLASGLIAAGFGSGSALFIPIIASIIRQQSYRA
                     AFLYTGLFQGVVIFAAAQILRNPGPEFHISKTASKCASGAVRRNSEQFSTGEMLRTPQ
                     FYTLYLMFVMMATGGLLVTAEAGPLAREWKISMAALTAALSLTRVANGVSRIFWGWIS
                     DRIGREPTMAIGFILQAGCLLSILFLGRLSGALFGITLVLTYFTWGEVFSVFPPITGD
                     YFGAANSASNYSFMYSAKGVSSIIGGGLAALLFEKFGSWSAAFYGSAVLAFLSGLMAI
                     GLRFAPLPKKSAATEAEAKAVLGTAPR"
     gene            complement(27707..28687)
                     /locus_tag="VFA54_00460"
     CDS             complement(27707..28687)
                     /locus_tag="VFA54_00460"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HZS49301.1"
                     /translation="MRFVVAGAGAIGAYLGAQMARAGFDVTLFARGAHLRAMQERGVR
                     VRSSDGEFEARPPVSGDPEAIGPADVVFLGVKAHSLTELAPRLKPLLGPDTAVVSMQN
                     GIPWWYFQQHGGPLEGLRLERVDPGGVISEAIEARRVVGSIVYLAAEIVEPGVVRHVE
                     GNRISLGEPGGTRSERCRQIADALIKSGLRCPITTRIRQEIWVKILGNVAFNPISALT
                     SATLAQMVRDPDVNLLVRHIMSEAECVAKRLGMELPVSIDQRIAGAEKVGEHKTSMLQ
                     DLESGRPMELEAIVGAVIEIGERLEMLMPSTRAVYACAKLLAQVRNRSRG"
     gene            complement(28684..30222)
                     /locus_tag="VFA54_00465"
     CDS             complement(28684..30222)
                     /locus_tag="VFA54_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HZS49302.1"
                     /translation="MTDAKLPGTLLDILQSAPGSRTAVIVPEAGIRITYDSLRSQVAA
                     MADALAAAGVQRGDRVAMALPNGLPAIVSFLAASIAGTAAPLNPNYRQEEFSFYLEDT
                     NAKVMLLPAEGAEEARRAAGGRIPILAVEMGADGSVRLQSPSQGVSASAPSPEDIALI
                     LHTSGSTGRPKRVPLKHRNLAVSIRNIANTYELGPDDISLAVMPLFHVHGLMASTMST
                     LFTGGTVVVPSRFNPLSFWRTVRDYRATWYSAVPTMHQLLLARLGKDSERPAGAEFLR
                     FIRSCSAPLPVEAAQRMESVFGAPVLEAYGMTEASHQMASNPLPPRVHKFGTVGPGTG
                     VRISIVDPAGRHLPAGQHGEVVIQGPNVIQGYENNPEANASSFVDGWFRTGDQGVLDS
                     DGYLRLVGRIKELINRGGEKIGPPEIDEVLLAHPAIAEAVCFGVPHPSWGEEVEAAVV
                     LKEGESVTERDVLAYCRERLAEFKSPKKIHIVRQIPRTATGKVQRRAVAAALGPQSSD
                     RGAQ"
     gene            30461..32185
                     /gene="recJ"
                     /locus_tag="VFA54_00470"
     CDS             30461..32185
                     /gene="recJ"
                     /locus_tag="VFA54_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZS49303.1"
                     /translation="MARWIRTSADPALVGSLAKELAIPELISRLLILRGITGAQQAAR
                     FLRPSLSDLHDPYRMLGMRAAVDRLRRACATGEKILVYGDYDADGTTAVVLLRKAIEL
                     AGGTADFYIPHRLRDGYGMRDDVMEHAARQGIRLIVSVDTGIREAVVVEKANGLGIDT
                     IITDHHLPEASPSRAPAALAVLNPNQPGCGYPDKNLSGVGVAFKLAQALLGSLDWPPA
                     RLEKVLRSMLRIAAIGTVADVVPLVGENRIIVKFGLHALGRPVNPGLKALLSASGIAE
                     GRPPTAANIAYWLAPRLNAAGRMDTASEVIELFSAADAERGAKIAERLCRLNAERQAA
                     EARIVEEILSEISAAPERAFPEQPPAAIVAAGEGWHRGVLGIVASRLVERFYRPAVVI
                     AISEKEGMAHGSGRSIPPFHLLEALESMADLFARFGGHRSAAGFSLPAARLPEFRSRF
                     LSYAAARLNPEDCIPSIAIDADLNFQEITCELLGELVQLEPHGAGNPEPIFAAAGLEL
                     QHPPRVLREKHLRLTLRQANRSFAAMGWRMAGIAASLIEKTVLNAAFTLERDDGGCRL
                     ILKDLAWP"
     gene            complement(32313..32750)
                     /locus_tag="VFA54_00475"
     CDS             complement(32313..32750)
                     /locus_tag="VFA54_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZS49304.1"
                     /translation="MRRSFDQLFESFFNATPRRASGQTASSDWVFAPAVESGWTDDYL
                     NLRVVIPGVTEKDMKVTVQGNQLYISGERKAPENFGKEGYVWNQIPYGKFERVLDLPA
                     GLDLDKLQAHLHDGLLDIRIPVASAMKPKQIQITAAEPKEIAA"
     gene            complement(33069..34037)
                     /gene="hemB"
                     /locus_tag="VFA54_00480"
     CDS             complement(33069..34037)
                     /gene="hemB"
                     /locus_tag="VFA54_00480"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZS49305.1"
                     /translation="MPFPTVRLRRLRQSGLLRSMVQETRLTPANFIYPLFVCPGEGVK
                     REISAMPGNYQMSVDQVVRECGEVAALGIPAVILFGLPESKDAEGSGAYASDGIVQRA
                     IRAIKREVKNILVVADVCLCEYTSHGHCGRVVGNDVDNDSTLELLSRTALSQAQAGAD
                     MVAPSDMMDGRIAAIRKTLDAHGFFNTPILAYAAKYASVFYGPFREAAESAPRFGDRR
                     SYQMDPANRREALREIALDIEEGADIIMVKPAMPYLDVICQARKQFSAPIAAYQVSGE
                     YSMIVAAAQRGWLDLDRAMWESLTAIRRAGADMILTYFAKTAARAF"
     gene            complement(34126..>35184)
                     /locus_tag="VFA54_00485"
     CDS             complement(34126..>35184)
                     /locus_tag="VFA54_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZS49306.1"
                     /translation="VIGPSVLGWVNPDQFLSALAELGVIFLLFTVGLEIKPAQIFQVG
                     GAALLVAAGGVAAPFIAGWAIMLWWNHSSVEAIFVGASMVATSVGITARVLASLGLMN
                     ERASQIILAAAVIDDILGLLVLAAVSGVAKGGLHYAELAGTAALAFGFTIFVAVVGTR
                     AMRRVGPRLAHLRQIRGEFAFALLLVFGLSLLATWAGVAAIVGAFLAGMVLAEHFEGS
                     DVHQLAQGATELVVPFFLVQIGLYLDIRVFQSLRTFGLALLITAAAIITKLAGCGLGA
                     WSLGFRDALRVGMGMAPRGEVGMVVAQIGLTMGVISGDAYAVVVFMAIATTLVAPPLL
                     RLLFPGARPAEAEADASL"
BASE COUNT         6971 a        10806 c        10523 g         6884 t
ORIGIN      
        1 cggcgtctcc gggaaggggc aacgcgggct gaccagcggc tggagcatgg cgtacacgcg
       61 ctccagcatt ggctgatcga atcccgtgcc gacattccct gcccacacga gctttccctg
      121 atcgtacacg ccgagaacga gcgccccgaa gtagccgccg cggtcgccgt gggtgtagcc
      181 gcaaatgacg aattcctgct gggaaacgat cttgatcttc agccaatcgc ggctgcgcct
      241 cggttcgtag cggctgtccg ctcgcttggc catgattcct tccaggccct ggcggcggga
      301 agcctcgagg atttcccgcc cgcttccggc aaagtgctcg gaatagcgga gaacaggaca
      361 aggctccagg atggatgcaa ggatgcgctt gcgctcgagc aacgccgcct ggcgcagatc
      421 gtagccatcc aaatacagca aatcgaatat aaagtaaacg accggccgcg tccgggagag
      481 ctgcgcgatg gcattcggat cgctgaccat gatccgcggc tggatcagtt cgaagcgggg
      541 cctcccctgg gcatcgagga cggcgatttc cccatccaaa atggcctctc gggcggcaac
      601 ataatgaggc acaacgctga gctccgggta ttgtgcgtcg gcgggattgc cgttgcgaga
      661 caccaggcgg atcttctggt cctggaggaa gcagatggcg cggaccccgt cccactttac
      721 ttcatacagc cagtcgggac cttccggcgg ctctccggcc gaaaaggctt gcatcggaac
      781 aatcgaagcc ggcattgggc cgcgaacggc ttcggagggc atggcaatgt tcttccggtt
      841 gcgcgctgtt ggagagcgcg aaacgaaagg cggcatcccg cgcgcaattt cttcctgcgt
      901 gcggccggtc aaagcgctgc ggggatgctg ctcggggtcc cagcccggct gtgcggcgct
      961 gtctttcttt ttcagcagaa gccattcgtt tcccttaccg cggtttttca tgcggaccag
     1021 cgcaaactcc ccgcgcagct tctccccatg caaccggaac ttcaaatccc cgcgttcgat
     1081 ctgctgctcg gcagcggcgt cccccaccaa ttcgtaaaag cctcggtccc agaccatgac
     1141 gctgcccgcg ccgtagttgc cttccggaat attgccctcg aaatcgccgt attccagcgg
     1201 atggtcctct accatgaccg cgaggcgctt ttcggaagga tcgagcgtcg gccctttggg
     1261 aacggcccag gatttcaagg cgcctccaat ctctaaccgg aagtcgtagt gcagccgccg
     1321 cgcatggtgg cgctgcacat aaaaccggcg aaccgcgctc tccggacggg gcggcgaggg
     1381 gagcggctcc ggcgtcttgt tgaaggaccg cttgcgggtg taatcctcga gagacattta
     1441 tttccaggga cggcaggaag agcggcagga cctgcgggca ttgtgtaccg tcctcgtcag
     1501 accgagcccg gaatgtctcc ggctaccgtt cgccatttca cttcaaaaaa aacagaaagt
     1561 tgcaaacctg tctttctcta tgatatcggt gtttacatcc cttagcaggc gataaggaat
     1621 acgaatggcc accacagttt ggaaagggca cttaacgttc ggtctggttt cgctcccggt
     1681 gaagctgtac agcgcggcgc gaagcgaatc ggtcagcttc aaccagctcc ataaaaccga
     1741 taactcccgc gtcaagcagg tgatttactg ccaagccgaa gataagccca taccgcgcag
     1801 cgaaatcgtc aaaggctttg aatacgaaaa agacaaatat gtcgtaatcg aagacgaaga
     1861 gatcaagaag gtcgctccca agacggcgaa gaccatggaa atcctggaat tcgtgaaggc
     1921 ctcggaagtg gatcccctat acctggagag ctcctattac ctggcgcccg atgaggccgg
     1981 cgaacggccg tacgcgctgc tgttcgaagc tttgcgccgg accggctatt tgggtctggc
     2041 gaaagtgacg atgcataacc gcgagtacat tgtgatcctg cggccggggg atcgcggcat
     2101 cctgctgcat actatgtatt acgccgatga gatccggcga gtcgaagagt ttcgcaccga
     2161 tacgagcgtc atcaaagaaa aggagctgga gctggcgacc acccttatcg agtcgctgaa
     2221 ggccgaattc catccggaaa aataccggga cacctatcgg gagaatttga aagcgctgat
     2281 caaggccaag gtggaaggcc aggagatcgt agaggcgccc gcgcccgcca tggcgccggt
     2341 catcgatatc atggaagctc tcaagaaaag tctcgacctg gccaaaaagc ctgttcagtc
     2401 cacgacggaa gcagcccggc cggccgctgt agagacccga accaagggca gaaagaaaag
     2461 cgcggccggc cagtagagcc gctcggcgcg aggctacgct cccagaatct taggaagcgc
     2521 atccaggcct gctttcgcaa cttgcccgtc ctggttggat tgcacgccgc tcacaccgat
     2581 agcgccgata atctggccat cgacggcgat gggcaggccg ccttcaaccg gaatggagcc
     2641 ggccaactgc atcatgccca ggcgccctcc ggccaccacg tcttcgagaa ccttcgtagg
     2701 gcgcttaaac cggattgcgc ttttcgcttt ctgttgagca acatcaatgc tgccgatttg
     2761 ggtatcgtcc atgcgctgca aatagatcag gttcgccccg tcatccacga tggcgaccac
     2821 cacgttccac tggttctttc tggcctccgc ttccgcgtac gcggccatct gcttggcgac
     2881 ctccagtgtg agagctttct tagtggcaag ttgtgcggcc atgccgaatg tcccctcctg
     2941 agaatttcct ggaaatcctc tatggtcgca tgaatctgga aaaggtcaat ggaccgcctg
     3001 gagcacttct tccgcgtaga gcgcgccatc cggcttttct tcgtgcttga aaaacagaaa
     3061 tacgtctttg ccttcgccaa gcagtttccg cgcctttccg gcgatcgcct tgcggtcttc
     3121 gggagaatac tcttctttgc ggagccggga gtaaacgaaa cgggctgtga tcacctccgg
     3181 cgcctcaaac ttgtctgact cagccagaca cagcgcgatg ttgcgctgct cgagcagccg
     3241 gtagacggga tcgatcagcc aggaggcgtg tcgaaattca aaagcgtacc gcagatcatc
     3301 cggaagagcc gcgagaaact cgcccagcag cttgacatca cacgggaatg cagggggcaa
     3361 ttgaaacagg atggggccga gccgccgggt ggctctcaag gggtcgatcg agcgaagaaa
     3421 tatcccggtg gcatccgccg catctcgcaa gcgcagaata tgagtgatgc gttgatggcc
     3481 cttgatggcg aacaagaagc ccggcggggt cagataaacc cagttctcca gtgtgctggc
     3541 ggcgggcatg cgccggaagg tgtagttgat ctcaacggca ttcagacgcc gggaatagta
     3601 ttcaagaaat cttttctccg gcaagtcggc cggatagaac gccggcttcc actggggata
     3661 ggcgaagccg gaggttcctg caaatagctg gggcatcgga tttacgcctt ccgcggcggc
     3721 atttgcgatc ggttttgcca tccggcggcc gcatccgggc gctattggcg gaagccagtg
     3781 ggagtcgaac ccacctgcga cacggagtgc cgcacgctgg ttttgaagac caggcccagc
     3841 accggccaag actggcttcc agacgcattc tagcagaacg gtccttcctg ccttcggcgg
     3901 gatgccgcgc gaaggctctc tcacgctgtg ctacggcgga ttagtccgtt cctccacaca
     3961 gcgccggcca ggatttaagg cgccgatgtt gctctctctc gaacccgcgg gatcgagcta
     4021 gcacaccagc ttttcacggc ctagcttaaa accgtttaga tttcccagca cggtcagcgc
     4081 aatctgtttt tggttgaacg attcgcaggc gatttgctgg acctcggcag ccgtgacctg
     4141 ctcgatggcc tcaatcaact cgtccagcga gaagaaccgg gggaagtaca tctcctggcg
     4201 cgccagatta gccatgcggc tggaggtcga ttccaggctg agcatcagac tgcccttgag
     4261 gtgatctttc gcccggcgca actcctcggg cgtaatccgg ccttccttga tttcgcgaaa
     4321 ttccttcaaa atcgactcaa ccacgcgctc ggcggcctca atggaggtgc cggcatagac
     4381 cgagaggcag ccggtgtcgc gatagggatt caactcggag tagacggagt aagccaaccc
     4441 ttgtttttcg cgaatgttct ggaataaccg ggagctcatg ccgcccccca gcagcgtatt
     4501 gagcacgtag cacgcgtatc ggcggccgtc ggccagcgga tagctcggaa cgcccaggca
     4561 aaggtgcact tgctcgagcg ccggcttgtc ccgaagcgag ataatggcgt gcgtcaccgg
     4621 cgtccggtcg tcgggagggg gcgggttgcc gtccagatca ccgaacgcgc gttcgaccag
     4681 ccgcatgagg tcgtcgtgat ccagccgtcc agcggcggtg accagaatgt tggatggcac
     4741 ataaacacgc ttgtaatact cgatcagcga ttcgcgatga aagctcttga tggtcttctt
     4801 gctgccgatg atgggtttgc ccagggagtg atccttccag aaattgccgg agaaaatctc
     4861 gtgcaccagc aggtcgggat tgtcctcgtc catcttcagt tcttcgagaa cgacgccctg
     4921 ctctttcagg atatcgtctt cctggaaaac gggatgcaac accagatcgg agagcacatc
     4981 gaaggccagc gggagatgct cgtcgaggac tttcgtattg aagctgatca tctccttggc
     5041 tgtaaacgcg tcgaggtgac cgccgactga atcaatggaa cgggcgatct cctcagccga
     5101 gcgcgtcgtg gtgcctttga acaccatgtg ctcgatgaaa tgcgaaatac cgttctggtc
     5161 cggcgcctca tggcgggacc cagtcttgat ccagatgccg atagagaccg agcgtacgtg
     5221 aggcatgacc tcggaaatga cggaaaggcc gttgggaagc tgcgctttct ctatgttgcg
     5281 agtttccgac tcgatcagag gcaccttatt ttccagaaga aaacccgaaa cctggcgcgc
     5341 cgccggatcg gcggcacatt tttagcttag catagccgat ccgaaacgta cttcttgata
     5401 taagactatt attttcaata ggatatatga acatatcgga ccggaaaacc caagctgccg
     5461 gccctttgcg ggctgcgcct ccccccgggg tgcgggcctt gccaagcccg cctaaagtcc
     5521 agggagccac tgggtgataa tggaatcagg atgccgctgc cgctagtcgg gcttgatgaa
     5581 aatgacctct tgcagatcgt gaccgcgaga ggcgaggcgc catttcgggc gcgccagctc
     5641 tacggaggga tttaccgttc ccatgcagca gtcttagagg atgtacgagg cttgccggca
     5701 gtcttccggc gggccctcga gcaggaattc gaagtcggcc tgccggcgat tgcccgcgag
     5761 tttcgatcga gcgatggcgc catccggtac ctgctccggc ttggggatgg gcagacgatc
     5821 gagactgtgc tgatgcccga agaggatcgg gatacgatct gcatttcgac ccaggtgggc
     5881 tgccctgtgg actgccggtt ctgcttcacg gcaatgctgg gtctgacacg gaacctgtcg
     5941 gcaggggaaa tcgtgggcca ggtgctggtg gccgtgcggc agaacggatt actggcgcgc
     6001 gacacgaggc gccggctgaa tatcgtcatg atgggcatgg gcgagccctt gctgaacctg
     6061 cccaatgtag tcaaggctac gcggctgcta accgacccgc agggcctcaa tctttcgccg
     6121 cgccgcataa cggtttcgac ttccgggatc acacccaaat tggccgaact ggggcgggag
     6181 ccggtcaggc cccgattggc gatctcgctg aacgcctcca cccaggaaat gcgcgaaaag
     6241 ctgatgccga tcacccggaa gtacaagctg gcggaactac tagaggcttg ccgcgcttat
     6301 ccgctgcgcc cgtgggagcg attatcgttc gagtacgtat tgctggccgg ggtgaatgac
     6361 tcagaccagg atgcccgaag ggttgtgcgg ttgctttcta acttaaagtg tatggtgaac
     6421 ctgatcgcct tcaatccggg gcctggtatt gacttcaaaa cgcccgaacc cgagcgcgtc
     6481 ctcgccttcc aggcgctcat tcagcgcagt ttgccctgtt ttattcgcaa gccgcgtggc
     6541 cgcgatatct acgccgcctg tggccagttg aagaggatgg gccaggaggc ggcggatcag
     6601 ggaccgaacc ccatcctcag cctttgatgt cccggaactt ctcttcgatt tcgcgtttgg
     6661 cttccggccc gacaggaatg agcggcagcc gcggcggccc gccatagtag ccgttcaaat
     6721 ccatcgcgta ttttaatcca gggatgccgt atttggtggt taccgctacg gcggccggcg
     6781 taaaccgccg ttgtaaatcg gcggcggcgg cgtattcccg cgtgcgcacg gcttcgtaga
     6841 tcgagatgca ggcgtatggg gcggcattgg cgaaagccaa aattgcgccg gcagcgccag
     6901 ccatcaggga cagccacaaa gtgggcgccg agccgaccag cacctggaag ccctcgggcg
     6961 cgtcgcgcac catctgcatg gttttttcca tgttgcccga gctctccttc acggcaatga
     7021 tgtttgggtg ctcggccagt tcgattacgg tctgggccgg aatgtcaatg ccggttactt
     7081 gcggccagtt ataaataata atcgggatct ttgcctggtc ggccaccgcc cggaagtaca
     7141 gagcctgagc cgcgccgttg ctaatcaggt tcttgtagta atgcggcgtg cggaccatag
     7201 ccgccttgta gccgatggat gcggccatat tcgtgagctg cacagtttcg cgtacgcttt
     7261 ccgcgccggt tccggcaatc agcagctttt ccggggcggc ggcttcgcga acgccatccc
     7321 aaagccgaac tttttcttca cacgtcagca tgacgctctc cccggtcgag ccgcacacaa
     7381 cgtatccggc gagttgggtc cggttccact tctccacgtt gtgccggatc ttggccatat
     7441 agagatcgcc atggtggtcg aaaggcgttg cgataggcgg gaagatacct tggagtttca
     7501 tcgcttgatt gtagctggga gcggcgtggc ccggcgcagc ggttgttcgc cgccaggggt
     7561 cactggcgcc cttcgtgatc gcgagggacc aggaccttac acttcgagac actcagcggc
     7621 acggatttcg gagcctgccc cccttcctta tgatgcaaca tccattcaag gcgccatgat
     7681 ctcgggagcc agcggcagat ctatggtttg cttctgtccg ggtctcaggt ctaccgattt
     7741 tccgtagctc tcggcggccc gccggactcc gggatcttcc cactcgaagt tgcccgaggc
     7801 cggaaaagcg tagacggtat aggatcctgg cggaatgccg ggaatcgtga aagcgccgcc
     7861 cggctgggtg aatccgcggc gctcggccag aaacggcgac gcttgcgcat cctgcgggaa
     7921 tagggcgatc acagccgcgg tgatccgatg ctctcccgat ggatccggca gcttcacttg
     7981 gccggccagg gtcgccgagt caaatccaat cacaatctcg aaccggctca ccgagccgtc
     8041 cccaggaatt tccagttccg gtgcgggaag atcccggccg ctcaaccgga cggctttgat
     8101 atagccattg ttggggatgc cgtcgagctg cacacggtgc attcctaccg aaagcccgtc
     8161 cgcccgaaaa gaaaaatcgg gaagaacccg gaagcttgac cggagctcgg gttcggactc
     8221 gggtggaaga gccaccaaca tgcgggcgtt ttcaattacc gtatccggag gatcgaacac
     8281 cacccggccc gtcacgctgc gccccgggcc gacggcaaca tatacatccc gaagatcttc
     8341 gctggttatc tgcacctggc gcgattccac cgcaacgtgg cggccggcag tggttttaaa
     8401 agcggtgatg aaatatgtgc ctggggtcag gccccgggcg cggtagccgc catcagcggc
     8461 cacgccgaag tgcatcggcg gaatctgatc gccggcgcgg ccgaccgaga cagcacagcc
     8521 gcggcaaggg ccgccgcttt ccgaatcaaa gaccctgccg gaaatggaaa atgtcctctg
     8581 gcggcggagg accatattga tttcggagag ctcctggccc caccgggtct cgagcacaac
     8641 agcctcggag gcgctcgagc cgttgggata aaaggtttcg gcaagccgca aatccccttc
     8701 cccggccgcc tgatcttcgg ccgggaaacg cacgccgatg atataccggc ccgccggaag
     8761 ccggtaaaaa cgaaacagcc cgcggtcgtc agtggcaacc gagtcggcgg gtgcaagcga
     8821 gcggcgtccc gcccgccata caaagcggta agctgagaca ctagcgcgga ccaccggctc
     8881 cccctctgca tcggttaccc gccctgtaat aatcgcagcc cggttgagct ttatttcgag
     8941 gcctctgcga atctgtccgg gctcgagcgg aaatgatgtt ggagaaccgg ctacaggacg
     9001 gtagcccgat ttttgcgcac gtatttgata gtttccggcg ggtaaatgct ggaaagagaa
     9061 ggacccgtcc gctgccgaaa gtattccttg ttggaaagga tgcccgtgca cttcagccaa
     9121 aatttccaca ctgacgcctt tcagcggctc gcccgtctgc gcgcagacga accggccgct
     9181 gatttcgccc tgactttcag aggcgcttgc ggatgagcct tccgccggag ccggaggacc
     9241 ggcccttgga attggggctt gcagcagcaa ggctgccagt atcgcgatcc caagattcat
     9301 ggccgtctcg gctgcctgat ggaagctccg gagctcggcg tctgagggcg ccggccggcg
     9361 gccggcactt cagaggaggt cagttccacc tccacctggg tgttgggatc gactacgatt
     9421 tttctgaccg ccgtgccagc ggcctgccaa gtctccgggg catagagaga ctccaccgag
     9481 gcgctgctcc aagcgcagat cagataggcc ccgggcagca gatcaggtat tgaccagcgg
     9541 ccccgctggt cggcctgagc cgtgcggaac cggatcggcg aaccgcctgt acgagactcg
     9601 ggcggcgctt ttgccatggc cacgcgggct tccggcaacg gagctccgct gcccggttcc
     9661 tttactatgc cactgaggct ggccgtgccg aacccggtct ctacctcaac atcgagcgca
     9721 gcgccttccg gcactgtgat ttcttttgcc tccgacggct cgccttccac catcatgtaa
     9781 actgccgagg gcgaaacaat gcggagggtg taactgcccg gcagcagatc ggggaattca
     9841 aatttgtaat cggggctgcg ggcagccgcc tctctgctgc gcgctcccag aacaaccgga
     9901 ctttggtcgg cgagctcgag aacgatcgct tccggccggg atcgacgagg gggaccttta
     9961 ggaaaagaca cgcggccgga aatcctcgcc acccgcgccg catgaagcag caccccggtc
    10021 atattgctgc gaacatcaac cgcctgccgg actatcgccg caatctgggc ccgcattccc
    10081 gcaggcacgg gacccggacg ggcggaaaca gtcagcacgt attcgccggc cggcaaacct
    10141 gaaagaaaga aatcaccctt ttcatccact gtggcgacgc ccccgttcgt catcccgatg
    10201 cccatgcgcc agtttagctc gtccggattg gcggagcggg cggcagcagc ggactctcca
    10261 ctgattggga tggcgagaat attggcgccc ctgatctgct caggatcggc gccggcgatc
    10321 ttaccgctga tggtgtaggc cggctgatct cccagagtca gatcaatccc ggtaacttct
    10381 gcgccggggg caacttcgat ttccttcgcg ttgctttcct gagccgtgcc aggaaaataa
    10441 gcggccgcaa gcggaggctt gcggcgaaac tgagggcggg ccaagagaat gtaactgccg
    10501 ggctcaagtc caaagatgcg gtacacgccg cgatcatcgg tgaccgcgcc aggggccagc
    10561 gcctggtgac gcccatcggc gtggatgacc cgccagcacc gaacctctat attttccagg
    10621 ggctcgccca gagcatcggt gatacggccg gtaatcacgc cgccagggaa caactcgaag
    10681 tccgcccgct ccagggtgtt atcactggaa aggtccagca gcaactccgg ctccgctccc
    10741 gccacggcgc ggccgaaata gcccggccgg gcggcggaca gcgcaacacg tttctcgggc
    10801 aggttttgga gcaggtaccg gccctgagaa tcggtttcgg cggtttggag tagatcgcgg
    10861 ccatcagccg cgcgagctac cacgtgaacg ccggaaatcg gcttggggat cttgccgtcg
    10921 tgcagccaga cggttccgga gatcgtcgcg gcccagacgc cagcggccgg caacagcagg
    10981 aaggcaagcc gcagcatgat acgagcctag catgcagaac cgacatgtat aatggccgta
    11041 ggaaccgatt catgaagcag aatcgaggcg ccgctattaa gcttcccaaa attcctaaaa
    11101 aagagcacac tcgatatgcg ggaatggcct accgccctgg aggccttagc gtgccgctca
    11161 aagtagtcga ggaggcggtc gagactgtga tccgcagacg cctggagaaa gagtgggggc
    11221 gtcccgtcag gcgctcggaa ttgaggagag accccagcct gccgcccctc accgagcacc
    11281 ccctgcttcg caagctcgct cgagccaaga atcgcaagac tgcgccgaca gcccctaaag
    11341 ggaagactgg caagcggcct gggattgcga gccactgaga gtgagcgagt acgataagag
    11401 catcttcctg aactgcccgt tcgatagcgc ctacaagccg atctgggacg ctattgtttt
    11461 caccctaaca caagctgatt tgcggccgcg ctgcgctctc gaagtggacg atgcggcgga
    11521 gaaccggctg gaaaaaattt accgcatcgt ggccgagtgc cgttacgcaa ttcacgacat
    11581 ttcccgcacc gaactcgatc ccgtccacgc cctgccgcgg ttcaacatgc ctctcgagtt
    11641 gggaatcttt ctcggcgcga aaaaattcgg ttcccgcaac cactcccgga aggcctgcct
    11701 gattctggcg cgcgagctct acgaacatca gaagttcttg tccgatctgt ccggccagga
    11761 tgttcacgcg caccataacg atccccgcct ggcggtgatt gaggtgagaa actggctgcg
    11821 cacagcggcg ccgcgcctgg atcttcgggg gggcgagcag acctggcgcc gctatcaagt
    11881 ctttcgggaa gaactgcccc agatttgcgg cgctatgggt ttgacggtgg aaggaatgac
    11941 gtttgttgac ttgctggcgg cggtgtccct ctggctgcgc agaaacccgt aaggtgattg
    12001 ccgcgccgtg cttcagaaca tcgtggagca ggcaatccaa tggcgctctt cccaacgccc
    12061 gtttttcgcc ttttcaaaaa ccttccaatc cgctctgccg cacaattgtc cgcaatagga
    12121 gcggatggct gtaagagcta gtgtgcggct ttggttgaag aaaaacattg gtcaaataga
    12181 aaagatcttt cgacttttcg aacagttctc tcggcttcgg gcggggaccc ggacggagca
    12241 cgtgcagctc tacaaactcc ttcacttcgc cggcgttcag caggtggtaa ggtttgtcga
    12301 gttggaagcg cggttgcaga atcaccgcaa catttttgcg cttgccatag tccgccatga
    12361 tctccgcgaa accggctgca tactcgtcag gcgggcggac gcacggttcc cgaggaacgc
    12421 ccggaaccgt tgtgcctgca accaggtaaa cctgattgtc accgggatga ctcagcggcg
    12481 cattcgccag caagagcgaa tagatcgcat aaacatcggc tcgccgcgac gcatcggaag
    12541 gcgaaccccc gccgccctgg aagccgctcg cggcaacaca ggtcaaaatc aggaggcagc
    12601 agcgctgaag catggcgccg atctttttat cataccgccg ccggattttg gtgctgcgct
    12661 caattagcgc accacccggg tcttgcgcaa atccgatgta cgcaggaaaa tggacgcctg
    12721 gaaaagagtt tcgcaagagc atgattcgga tttgccttcc ggtactcggt ttcgcgctcg
    12781 cggtacagct cgccgcccag ccggccatac aactcaacga cggcgacagc cacggcgacc
    12841 ctccctactt gcttgagccc ggctggaagc ctctgctgaa tggcagagac ctgagcggct
    12901 ggcacagcca ggacggcaag ccgctccaat ggttcacaac taccgccata cgctgggaac
    12961 ggctgctcgg cccgacgcgg ctgttcgcaa tcccggcgcc gggcggcaca atattgaaca
    13021 gcgctcaggg ccgtaccagc aacctggtga ccgaccagaa attcggggat gtcgagctgt
    13081 atctggaatt catgctggcc aagggatcga attcgggcgt gtacttgcac ggcctctatg
    13141 aagtgcagat ctttgacagc tacggctcgt gggagccgat gacctcatcg gattgcggcg
    13201 gcatctatca ccgctggatc gacgagcgcg gcgtcggcgg atcggctccc tcgcggaacg
    13261 cgagccgcag gcccggggag tggcaatcgt ttcaaatctg gttccgcgcg ccccgatttg
    13321 acgccgacgg gcggaaggtc gaggacgcac gttttctgcg cgtgctccat aacgggattt
    13381 cggtgcagaa agacgtagcc gtggacgggc cgacgcgggc cgccatggat catcccgaag
    13441 cggcggaaaa tcctctcatg ctgcaaggcg accacgggcc ggtcgcgttc cgcaacatct
    13501 atatccggcc cctccggccc atcatcgaga ggtaagccgg ggcgtcttgg atctcggttt
    13561 gccggagctc gggccttgta acagcgcctg gaaattcggc ggcttcctcc gcccgaatca
    13621 ggaaatctcc tcttttccat aagctgggac ggccgggccg ctatgttata ctgggaatgc
    13681 ctgtgcggag ccgaagtccc atgttcaacc gtataaagtt tggaattgtc gttggttcaa
    13741 cattcttaac cgccctcctc gtaatcggcg ccgtcatggg cgacagttct tcggctgacg
    13801 gcgcgtaccg ccaactctcc gtctacacgg aagttctctc ccgcattaag agcgattacg
    13861 tcgaggaacc cgatatcaaa caggtgacgc ggggcgctct gcaagggctg gtcgaatcgc
    13921 tcgatccaaa ttccagctac ttgaccgccg agcaatacaa agaatacgaa aagcgtaaga
    13981 gcagccagca ggctggcgtg gggctgatta tctcaaagcg cttcggatat gtgctggtgc
    14041 tagccgcatt gccaggttcg cctgccgcga aagccggttt gagcgtggga gatttactgg
    14101 aggcagtcga taaaaaagcg acccgcgacc tggcgctgcc gctgatcgag accatgctga
    14161 atggtcccgc tggcagcact gttactttag tggtgcggcg tgcccgccat gcggaggaac
    14221 ccgaagagct gacgttgacg cgcatggctg taccgactcc gccggtggtg cacaagctgc
    14281 tgacggataa agtcggttac cttgatgtga aagccttgac gcccgggaaa gccgccgaag
    14341 cgggccgcgc gatccgcgaa ttgatgagcc agggagtgga gcgcctcgtc ctggacctgc
    14401 gcaatgatgc gatgggcgac aatcaggaag ggctgaagct ggccaatctg tttatcgaca
    14461 acggcctcct gggatacctg gaaggacaaa aatatccccg caaggatttc atggctgatc
    14521 cgcgccaggc catctgcaag ctgccagtgg ccgtaatcac aaaccgcgcc actgccggcg
    14581 ctgccgagct ggccgccgca gcgatcgtgg accgcaaccg cgggcaaatt gtcggcgaaa
    14641 agacgtttgg gttggccgcg gagcaaaaga cgattccgat ggaagatggg gcggctatca
    14701 ttctctcagt ggccaaatac caccgtccgg gaggcaaggc catccaggac gggggcgtga
    14761 atcccacgct tcaggtttca gagactgata atgaagccgc cgtggacgac gacaacgctc
    14821 cgactccttc tccatcgcct tcgccgaagt ctcccgagga agacgaactg ctaaagaaag
    14881 ccatcgaagt cgtcaaaggg gcggctgccc gggctgcggc gtgagcgctt tatccttcct
    14941 tttgatttcg gcccgccttt ccgctatagt ggtgtagcaa attgatctct cgcgcgtccg
    15001 cggcttccta tacgcgccga ggccggcaaa ggaccaatca gggaatatga cgctgccgaa
    15061 cgccctgacg gtgctccgca tcttcttcgt ccccttattg ctggctgtct tgctgcgtaa
    15121 agaaatttat ctggagacgc cctggattac tttgagcaaa gagtggctgg cccttcccat
    15181 ttttttggcg gcggctctta ccgatttgct cgacggcatc atcgcccgcc ggcgcggcca
    15241 gatcagcacc ctggggactc tactcgatcc ggtggccgac aaactgctca tctccgcggc
    15301 tttcatttcc ctggtggaac tgggcgtcgt gccggcttgg atggtagtgg taatcgtggg
    15361 ccgagagttt gcagtgtccg gattgcgcag cattgcggcc gcggagggct acacgatcga
    15421 agccagcgag ttggggaaga cgaagatggt catgcaggtg attgccgtct ccctgctgct
    15481 cgcgggcctg cactggcctt tcgtcctgcg attgggaatc gtggcagtct ggctggtggt
    15541 gctcttcgcc atcgtttcgg ctgtggatta ctttcgcaag ttctggcgcc gcgtggatat
    15601 tcgcattaag gtgcgccgaa ggcgcgagcg cctcctgctt ttgcggaagc agcgcaaaga
    15661 tgttgccgct cactagcgtc gccagaaact ggctgctcgt ttcggcccgc tgtgcggtgg
    15721 aagcggccgc acgaggcgag atcccggcta tctctcaaat tcctcccgag attcccgcct
    15781 ccgaccggcc ttggctcgaa cagcctcacc cggtattcgt gagcctgcat ctggagggcc
    15841 gcctgcgggg ctgtgtggga cacgttggtc tcgaaattcc gttgcgctgc ctggttccgg
    15901 aaatggctta cgcggcggct tgcgaagata cccgattcca gccggtcact gccgaagaag
    15961 cactccgcat cgacatcgag attttcatac tgtcgccgtt tttctccatt cggccggaag
    16021 agatcatacc gggaacgcac ggtttgatcg tgcgccaggg ccagcgccgc ggcattctgc
    16081 tgccctgcgt ggccgccgag cataactggg acgccgtgcg ttttctctgc gagacatgcc
    16141 gcaaggcggg tctggcgccg gatgcctgga agcacggcgc cgtcatagag gccttcaccg
    16201 ccgaggtaat caagcaggct gcattggctg gcgcgagtcc ttgaacgcct cagctcggaa
    16261 cggcgaacct catccaaggt ccccttcctc cgccagcagg cgcaagagtt tttcaaaagc
    16321 gctcactgtc cggtcgatat cttccccgtt atgcacgcct gacacgaggc cgccgggcca
    16381 accaatcacg tcgacgccgg cgcagagaaa acctgcacga atcttttgca gtagttcagc
    16441 cggggctccg cctttgagct tcgtccacgg gaccttcccg gcgtgcacgt cttgcggtga
    16501 caccggcgca gatccggaat ttagatacat atggaaaccg gaaaagtcgc catacacaca
    16561 ccagcttgcg ccgagcctgg cgagcgcgcg attcatttca gtgcggattg ccgcggccgt
    16621 gcgattcgct tgcgcgatca tttccccatc ccgcagcagc cgcaatgtgg cgattccggc
    16681 ggcagccgac aagggcgacc cgttgaacgt gccttggtga ggcacagccg gcggctgaat
    16741 gccctcttcg gagtgatgat attccattac ctccaggatc tcggcgcggc ccgccagaac
    16801 tgcgcccgga taacctcccg cgacaatttt ggcgagggtt gtcaaatccg gcgtcacgtt
    16861 gtagtgcgct tgcgcgccgc ccggcgaaca gcggaaaccg gtgatcactt cgtcgaaaat
    16921 cagcacgatg ccgtgccgcg cggtgatctc ccgcaaggcc gatctccccc tggagcggaa
    16981 catggccaaa agtggcccca gtcggctcca gaatgagggc ggcaatatcc cggcgttgct
    17041 cacagatttg ccggacctga ttgatatcgt gcggctggca caaaatcacc gaaccggcga
    17101 tttcctttag gatgccgggc gccggatcag cgccgaacgc gacatgatcg tgccagccat
    17161 gaaagtggcc ggcgaagcgc atcagcatgg gcttcccggt ataagcccga gcaacgcgca
    17221 gagccagcag cgtcgcttcg gtgcccgagg atgtaaagcg catgcgctcc gcgccaggga
    17281 ccatctgccg aatcagctcg ctccattcga tctccagctc atgggaagcg ccgtagtgag
    17341 tcccccgcgc aagctgcgcg gctactgcct ctacgaccgc tggatgattg tggccgagca
    17401 ttaaagcccc gtggcctccg aagtagtcca cgtattcatt gccgtccaca tcccacttgc
    17461 gcgaccccgc cgcacgcccg acgaacagcg ggtgtggctc gagatagcgt cccaggtgtg
    17521 taatgccgct gggaaacagc tttagggccc gctggtaaag cttggccgat gacggcgttt
    17581 tctcggcgta ctcccgcagg atccgggact gctggagagg cactctgttt cgtgcagcga
    17641 tagggctcat tcgtccagtc cgaaggtgaa aatagagctt ccggcggcta cagaaaccaa
    17701 ctgcctgcct ctctcgaggt aggtgatcgg cccggcggca atcataccac ctgtattagc
    17761 gtgccatact tcccggccgg tttgagcatc cagcgcaaaa aactctcctt cgttgatgcc
    17821 gccaaaaacc aggccgccgc gggtggaaag aacgcctgac tgcggtttgc tggggagctt
    17881 gtgctcccat tggactgcgc ccgtctgtgg attaatcgcc cggatagagc cggcccccgg
    17941 gtcctcggga atatcgactg gcacactgcc tgcgtagcga ttgcccggcg agtaaacagc
    18001 gtccccgagt tgatatcgag tcgcgtaatc ccagaccgaa agatagaaca agcgagtgag
    18061 aggactatat gacggcgaat accaatttgt cgctccttgc acgccggggt acaccagcgt
    18121 cccgcgcgcc gttggatccg agcctggata cggcatgggg cgcccctgct tattgattcc
    18181 gccggcccac gtctggcgga caaacggctt cgccagaagg aattcgccgc tttcgcgatc
    18241 cagcacgtag aaaaaggcgt ttctgttggc ccaatacagc agcttgcgcg ggcggccttg
    18301 ccacaaagcg tctacgagca ccgggatctg gacagcatcc caatcgtgca cgtcgtgcgg
    18361 agtgaactga aagtaccatt tcagcttgcc tgtgtcggcg tccagggcca ccacgcaatt
    18421 cgaataggag ttgtcgccgg gacgcaccgc gccgttccaa tccggagctg gatttccgat
    18481 tccccagtaa atcgtattaa gttcgggatc aaacgatccg gtaagccaac tcgggccgcc
    18541 gcccgttttc caggattcgc ctgcccagcc gtcatggccc ggctcgccgg gacctggaat
    18601 cgcgttgaag cgccaaagat gcttgccggt tcccgcatca taaccgtcta caaagccgcg
    18661 aatgccgtac tcgccgcccg caataccggt cacgattgtg ttcttcacgg ccaaaggcgc
    18721 cgccgtaatg ctatacccca ttcggtagtc agcgacggct acatcccaaa tcacggagcc
    18781 agtattgcgg tccagcgcca gcaggtgggc atccagggtc cccacgaaga gccggtcccc
    18841 gagaatagcc agcccacggt tcacctgtcc gcaacagacg ttgatctttt ccgggaggct
    18901 ccggcgatag gtccataacg ggcggcccgt gccggcatcg agagccgaga ccgcattcgg
    18961 cggctcggtc aagtacatca cactgtccac aaccagcggt gtggcctcga ccttctcgac
    19021 cgttttcatc tggtacgccc atttgaatct gaggcgcgcg acgttttccg ttgtgatctg
    19081 gtcgagggaa ctgaagcgat tgccgttgta gtttccggag tacatcaacc agttctccgg
    19141 ctcgcgatcg gcccgaagca ggcgctcata cgtgaccggc ggagaggctg gcagggctgc
    19201 cttcccgcgg acaagcactg ggggaagcga acggatgaac tggataattc gccggatttc
    19261 actttcggga agaaagatcc caggcatttc cgcaccaggt atgcccttag agatgatccg
    19321 cagcaggtcg gcgtccgtag agccgtgccg gaagctggtt ttgcggagat cgggaccgcg
    19381 gccgccttcc cctctgatcc catggcacgg cgcgcaatgc gcgcgaaaga tcacagcgcc
    19441 ggccgccacc tcgccggaga caggcgcttg aacggacact gactgctgcg ccccaagcaa
    19501 gttcaggcaa agaaagagaa gagatgccgc aatcgtgcaa tacaaagggc ccttcgcgct
    19561 atcctgcggc gccatgctta aatgcagcat tatagcgaga tcatcagccg caattcttta
    19621 ttttctacag cgagagacgt cgagtattat caaatttgtc catgagaaga gcagttgcgg
    19681 ttgcgttctt tcttctgaac ctggcttcgt gttcgaggcg gcagaacacg caggaccctc
    19741 ctctggtgga ggaagagcat cccgagtcga tgctttcgac attgcacgtg gccgatccga
    19801 aggccgtacc gcagttgttg aagggctggc atgccgtcga gcagggaagc tggcgatgga
    19861 cgcaaaagac tttcgccgtc gccttgaagg ccccgtctcc ggggaagccg gcaaagctgc
    19921 aactgaaatt cgtcattccc ccgccgttgc tgtcgcgcct gcattccatc accctcaacg
    19981 ccagcattga cggggtttct ttgggtccgg aaacttataa ccaagcggga gaccagacct
    20041 atacccggac gatcgcgccg agagaaacct ccggcaattt gattcgggtc gattttcagc
    20101 ttgataaagc cgtgcctccc gacaaccgtg acaagcggga actgggcgtg gttgttacgt
    20161 ccgtcgggct ggagtaaatg cagccagccg cttctaccat cgagaccccg cggcgccacg
    20221 cggcgctccc gcaagccgcg ctgtggtgcc ttcccctgct gctctgcctg gccttctttt
    20281 ggcgaagcct gtttagctgg ttccagcagg atgatttcgc ctggctttac ctccgcttgc
    20341 agctccattc cgcctcgggg ctatggcgct tactgatgga gccgagggcc gagggaacga
    20401 tccgtccttt gagcgagcgg ttgtttttca tcgtatttcg ctcactcttt ggactgaacg
    20461 cctttccgta ccatttgttc gttcttctga ctcagttcgc caacctggcg cttctcctcg
    20521 cactggcgcg ccggcttacc gcctcgctcg gcgcggccag cctgacggcg gcgctctggg
    20581 ccgtgaacgt ctcgctgctg acgccgatga tgtgggcctc ggcgtacaac cagattcttt
    20641 gcgcgttttt ctttctcagc agcttgctgc tgttcctcaa acacgtcgag accggccgcc
    20701 agcgctttta tgtcgcccag ggctgcacat ttttggtcgg cttcggggcg cacgaatcga
    20761 tggcggttta tcccctgata ctgctcggtt actgcgcgct cttgaaccgc cggcacgcct
    20821 ggaaagcggc gccgctgttc atcccatcgg cgctctatac cgcggcgcac ctctggctca
    20881 tcccacattc cgcctctggc ccttatgcgc ttcacctgga tctgagcgtc attccgacgc
    20941 taaaggccta ctggagctgg gcgctaggcc ccccgttgtt cgcggcatcc ttgcattggc
    21001 ccgggcgcgc aagcacggcg ctctgggttc cgctcaccct gtccctgggg gcggcaatga
    21061 tgcgcgccgg caggcctaag cggcgggcag cgcttttcgg gttggcttgg tttttcctga
    21121 cgctggcccc ggtcttgcct ctgcgcgacc atcgcatgcc gtattacctc gtgatcccag
    21181 cgatcggtct ggcgctggct gccggctcgg tcgccggctc tgcgccgcga tgggcggcag
    21241 cggcctggtt gatggtctat tttgggtgct cgagcgcttt cctaatccgc gaggcgccgc
    21301 tcgtgtgcga acgcagccgc gcggccaaac gctttcttga ggcgctccgg caggcccgcg
    21361 cctcgcatcc tgataagacc atcctgttga ccggagtgag cagcgagttg ttctattcgg
    21421 tgatctatga tcacggaccg ctggcggttc gcttatcaga ggtctatttg gcgccggaca
    21481 gcgagggcat cgctccccag ccgggtctgg atgccgtttc gaatttcgag ctgccggcgc
    21541 gcatcacgct ggccgcgctg cgggccggcg acatcattgt gtacgatgcg tctggcagca
    21601 atctcaaaaa cgtgacgtcc ttgtatgccg tgaacgcccc cgagcgcctc aaggacgctc
    21661 cgccccggcg cattgatctg ggcgagaaac tgattgccgc gcagcttgga cccggctggt
    21721 atgagcacca ggtaaaccac cgatggatgg gagaacgcgc cgaggttcgc ctggccgccc
    21781 cgcgctcggc cggcgagcgg ctgcacatcc gagcgattta cccggatcat taccatgtcg
    21841 ggaccatcta cctgaccgtt ctcgtgaatg gaatccctgc cgggcgtgcc gcgatttgcg
    21901 acagccgctc gagcgagctg acattttcct tgcctgcttc cgtgaccggt ttaagtgaaa
    21961 tgacggtgac gctggaagcg ggtccgacct ttcggcctcc tggcgatacg cgtaatctga
    22021 gcctcgcgtt tggcgtgatc gaattgctgg aataacttgc gtcggcggga ccggaggtat
    22081 tctgcattac tttgcgcacg cctgtgctac attggccgta tgagcaaggc ctttgacgtc
    22141 gttggggtgg ggctcaacgc cactgacacg ctgctgctgg tgccgcattt cccggaatac
    22201 gccggcaagg cgccgattgt ggaagaactc atgtcgcccg gcggccaggt ggccagcgcc
    22261 atagtgacct gccagcggct cggcttgcgc acgaaataca ttggcacggt cggcgatgac
    22321 gagcgaggcg ctatccagat gcagagtctg ctccaggaac acatcaacgt cgaccacgtg
    22381 cagatccgcc aggggtgccc caaccagtcg gcttatatca tcgtggacca gcgcaccggg
    22441 gaacgaaccg ttttctggag gcgcgacgaa tccctgcgaa tcgacccgtc gcaaatcgcc
    22501 gcggaacaaa tcacctgcgc ccggctgctg cacattgacg gtcacgacac cgccgcggtc
    22561 gagcacgccg cccgcatagc gcacgaggcc ggcatgccgg tgacggtcga tgtggatacg
    22621 atctatcacg gttttgaccg tgtgctgcgt tacgtcgatt acctgatcgc cagctccgaa
    22681 tttccggtcc agtggaccaa ccagcgcgac cccttccgcg ccctcgagct cattcagcag
    22741 gaatatggca tgaaggtcgc cgccatgacg ctcggcgcct acggatccct ggcttactcc
    22801 gaggggcgct ttttctattc gccggctttc gtggtcaatt gcgtcgacac taccggcgcc
    22861 ggcgacgtct tccacggggc gttctgttac gcagtgctgg aaaacttgcc gctggatgaa
    22921 gcgctcgaat ttgcaaacgc catggccgct ctcaactgta cggtgctcgg cgcgcgcggc
    22981 ggcatcctgg gcagcgccga agcgcggcgg cttatggcga gggccgagcg gcggtccaac
    23041 aaagacttcg agatccgggc gtcgatcccc gtcggttgaa ctcagccttt gcgatgatcc
    23101 cccgtgttac gctttacacg cgttccggct gtcacctgtg cgacgaggcc aaggagcaga
    23161 tccggcgcgc ccgccgccgg actcccttcg atttggaaga gattgacatt gactccgacc
    23221 ccgggctgcg cgctctttac gacgaagaag tgccggtggt ggcgatcaac gggaagaagg
    23281 ctttcaagta ccgggtgaag gagaaagaac tactcaccaa attgagaagc cgctcgtgag
    23341 ccgttgccgg cgggggccga ttcgcggccg cgggcgaaca gggattgccg gatttcgctg
    23401 catccgccgc ctccctgcaa aattcttatg gctttagatc tgctggccat tgccgctcat
    23461 cgcgacgata ttgagcagac ctgcgggggg actctgatcc gcatggctga gaaaggctac
    23521 cgtaccgggg ctctggatct caccgctgga gagatgggaa cgcgcgggga tgccgaaacg
    23581 cgggcccggg aagcggcgga agccgcccgc gaaatgctgc tcagttggag aggaaacgcc
    23641 ggcctgcctg atgcgaaact tgagctgtcg ctcccggcca aactcgccgt cgccgccaaa
    23701 attcgcgagt tgcgcccccg ggccgtcatt cttccatact gggagggaag gcatcccgat
    23761 cattaccgca cgtccactct gtgctatgag gcttgctttc ttgccggcct caaaaagctg
    23821 gatttggccg gcgaggcatt ccggcccgcc aagattcttt acgcatcgct ctatgcgaat
    23881 gtccggccga gttttgtcgt tgacattact ggacaattcg agcgccggat gcgcgctctg
    23941 ctgtgctacc ggtcccaatt tgaagatcag ccggccggcg ccgatttgtt tccggcccgc
    24001 gacgaactga tcgagcggat tcgctccgtg gcccgttact atggaatgct gatcggggtc
    24061 aaatacggcg agccgttcgt tgtgaaagag atgatcagcg tggacgacgt ggtaaagatg
    24121 cccgtgcgca gcatttgaag aggctcattt taccggcggc ttcccggaaa cgcggcgccg
    24181 caaacccgaa atacggcgca agaaaatcag gctcccgcgg ccaaacgtca cgaattgcgc
    24241 tggttgctgg ccacgaaccg gtctacgcgg ctctggtaca ccttcacttt gccttccttt
    24301 tccatccgct tcctgagctc gtctttgaag atgtcgaacg cctcgttctg gcgggttccg
    24361 gtcatggtgt cgtgcagcac cttgctctcc gaggcgaaca gcgacatatc ggcgccgaca
    24421 cgctcggtca cctgataaac agcgtagtcg gcgccggctc ggaccgggcc caccacgcta
    24481 tggggcggca cgttgaaagc gtcggaaaca gtggacgctc ctcccagccc gggaatggac
    24541 ccgtcgcggg tgaacaggtc gctgcttttc acctccacgc cgaattccct ggctgccttc
    24601 cggagatcgc cgagcgcctt ggcccgttcc gcaagcttat cggcccgcgc cttgacaagg
    24661 tcgcgagcct tcgccgtgtt gcagtcgttt ttgaccctct cggcgacttc agaaaactcc
    24721 gcctgccgcg ccggagctac ttccgtgatg acggccataa tggccttgtt tccggtcatt
    24781 tgaatcgggc cgactaggct gcccttagcg gccgagaaga gcgattccat cacgcccgtc
    24841 tcattgccca actgcgggat cgcagagttg cggtcaagca tggtcgcggt gttgaccgaa
    24901 agccccacct cgcgcgccgc ctcctgcaag ttctggccgt ggcgcgtcgc ggcggtgcgg
    24961 agccgatcca tcaaggcgga tttttccgcg tcgctgcggt cgcgcatgaa ctcctcgcga
    25021 atggtcggct ccacttcctg gaacggcttg agccgcgccg tttctttttc cagcaccttg
    25081 atgatgtgaa aaccgtattg cgtcttcacc agatcgctga cttcgcccgg cttcatactg
    25141 aacgcttttt gctcgaattc gggcactgtc tggccgcgcg taatccagcc gatgtcgccg
    25201 cctttggcgg cgctggcggt gtcttctgaa ttctccttag ccaatgcggc gaaatccttg
    25261 ccggcccgca cctgcttcag cacgtcctga gccttcgcct cgatctgctt gacttcctcc
    25321 ggcttcttat cggtagtttt gaagaggata tgcgttacgt gcaccctatc ctgcacgcgg
    25381 aagcgctctt tgttctcgtt gtaatatctc tgcaactctt cgggagtcac ctttacgcgt
    25441 gacgcgaccg ccgcgtcatc gatgacgaca tattggaatg agcgcttctc cgggaccata
    25501 tacatgttgc ggttcttctt gaagtattct tcgatttccg ccggggtgga agaaaccgag
    25561 gacataacgg acgcgggcgt gaccagcgca tagtcgatct tcaccttttc gttgcgccgt
    25621 ttgaactcct gctccacttc cgccggcgtc accaccacgc cgtccgtcac caggcgccgc
    25681 agtttggaga gggcaagatc cttgcgggcc agcgcttcaa attcggggac cgacttctcg
    25741 aaaagctggt atacgtattg cgcgtactgc tccttgccca caaacttccc gtcgggaaag
    25801 agctgcggcc aggaagcgcg gaggacgttg gccagctctt cctcggtcac attcagcccg
    25861 agccgctccg cctcttgcag cagtacggcg tcgtcaatca tgttccgcac gacctgcgga
    25921 gccagaaaat tgtaggcctg cgaaggcaag cgttggctcc ggatcgtgtc ctgaagctgg
    25981 gcctgagctt cggcctgtgt gatctcctct ttgccaacct gagccagcac cgtatcgttg
    26041 gtggatgtgc cggcgttgcc atagatgcca ggaatgaggg tgatcaccat tgtgatgcac
    26101 acaattccca gaatcgaacc caaaaaaatg cgcatcactc gcttgcggct gcggaaaagg
    26161 taaaacataa ctttccttcc gggagagttc cagaccttat tttaacagag cggctcgcag
    26221 cctagaaatg aacggccggg ggcgaagcgt cggatccgca gcttttcgag aaaatccgcg
    26281 cccttcaggt tcaccggttt gctggctcga ctttttcgta cctcgccgga gcgcttctct
    26341 gaccgggagt cgctcagcgc ggcgccgttc ccagcacagc tttggcctcc gcctcagtgg
    26401 ccgccgattt tttcggcagc ggcgcgaagc gcaagccgat ggccatcagg cccgacaaaa
    26461 aagccagcac ggcgctgccg taaaatgcag ccgaccagct tccaaatttt tcaaacagca
    26521 gcgccgcaag ccccccgccg atgatggaag acactccttt ggcgctatac atgaagctgt
    26581 agttggaagc cgagttcgcc gctccgaagt agtcgccggt tatgggcgga aataccgaaa
    26641 aaacctctcc ccaggtgaag taggtgagca ccagagtgat accaaacaga gcgcccgaaa
    26701 gccgtcctaa aaacagaatg ctcagaagac aacccgcttg caggatgaag ccgatggcca
    26761 tggtgggctc gcggccgata cggtcagaga tccagcccca gaagatgcgg ctgacgccgt
    26821 ttgccacgcg ggtcagcgac agcgcggctg taagcgccgc catcgagatc ttccattctc
    26881 ttgccagcgg acccgcttcc gcggtcacca gcaatccgcc ggtggccatc atcacaaaca
    26941 tcaggtacag cgtatagaac tgcggcgtac gcagcatctc tccggtactg aattgttcgc
    27001 tgttgcggcg gacagcgccg cttgcgcact tcgacgccgt tttggaaatg tgaaactcag
    27061 gtccgggatt gcgcaggatc tgtgccgccg cgaaaatcac tacgccctga aacaggccgg
    27121 tatacaggaa agctgcgcga taactctgct gcctgatgat gctggcgatg atcgggatga
    27181 acagcgccga tccggaaccg aatccggcgg cgatgagacc tgaggcgagc ccgcgcttat
    27241 ccggaaacca cttcaaggcg gccgcaattg agccgctgta aacaaatgcc gcgcccactc
    27301 cggcgatcgc atagaagagg tacaactgcc agagttgcct tacaaacccc aatgttgtcc
    27361 aaccgacgcc gcacagcacg gcggcgatcg aaatgaagat gcggggcccc atgcggtcga
    27421 tcagccagcc ctcgagcggc atgacccagg tctcgcacac cagaaacaaa ctgaagcccc
    27481 attggatgtc ggaaagcttc caccccgtac ccgcctggat gggttggacg aacaaagtcc
    27541 aggcatattg caagttggcg atcatcacca tcgccaccac agagccaacc agttgcaccc
    27601 atcgattttt cataagccag atcacccacc gctcgcagcg gtccgagcaa aagaacgcaa
    27661 agcgtcccgg gacgctccgc gagccggcgg cagcccgcgg cgcgtctcat ccacggctcc
    27721 gatttctcac ttgcgccagc aattttgcgc atgcataaac agcgcgcgtg ctaggcatga
    27781 gcatttccag ccgctcgcca atttcaatca ccgcccctac aatggcttcc agttccatcg
    27841 gacgcccgga ttccagatct tgcagcatcg aggttttatg ttcgccgact ttctcagccc
    27901 cggctatacg ctgatcgatg gaaaccggca attccatgcc gagccgcttg gccacgcatt
    27961 ccgcctcgct cataatgtgg cgcaccagga ggttcacatc ggggtcgcgc accatctgcg
    28021 ccagtgttgc gctggtcagc gcactgatcg ggttgaacgc cacattgccc aaaatcttga
    28081 cccagatttc ctggcggatg cgcgtggtga tcggacatcg caggcccgac ttgataagag
    28141 catccgcaat ttgccggcac cgctccgacc gcgtcccgcc gggctcgccg agcgagatgc
    28201 ggttgccttc gacatgccgg acgactcccg gctcgacgat ttccgccgcc aggtagacga
    28261 tcgaaccgac cacgcgccgc gcttcgatcg cttccgaaat cacgccgccg ggatctacgc
    28321 gctcgaggcg cagcccttcg agcgggccgc catgctgctg gaagtaccac caggggatgc
    28381 cgttttgcat gctgacgaca gcggtatcgg ggccaagcag cggcttcaga cgcggggcaa
    28441 gttctgtcag actgtgggcc ttgacgccga gaaacacgac gtcggccggg ccgatggctt
    28501 ccggatctcc ggagacgggg gggcgggcct cgaattcgcc atcactcgag cggacccgga
    28561 cgccccgctc ctgcatagcg cgcagatgcg cgccgcgagc aaacaaggtc acatcgaagc
    28621 cggcgcgagc catttgagcg cccaggtagg ctccgattgc gccagccccc gcaaccacga
    28681 atctcattgc gctccccgat cggaagattg gggcccgagt gcggccgcca ccgccctccg
    28741 ctgcaccttc ccggtggctg ttcttgggat ctgcctgacg atatggattt tcttcggact
    28801 tttgaactcc gccaggcgct cgcggcaata cgccagcacg tcgcgctcgg tgaccgattc
    28861 cccttctttc agaacaaccg ccgcctcgac ttcttcgccc caactcggat gcggaacccc
    28921 gaagcagacg gcttcggcga tcgcaggatg cgccagcaac acttcgtcga tttccggagg
    28981 tccgattttt tccccgccgc ggttaatgag ctccttaatg cgtcccacca ggcgcagata
    29041 gccatccgaa tcgagcacgc cctgatcgcc agttcggaac cagccgtcca cgaaagagct
    29101 ggcgttcgcc tcggggttgt tctcataacc ctgaatgacg ttcgggcctt gaatcacgac
    29161 ctctccgtgc tgcccggccg gcagatgcct ccccgcggga tccacgatgc tgatgcgcac
    29221 tcccgttcca ggtccgacag tgccgaattt atgcacccgc ggcggcagcg gattggatgc
    29281 catctggtgg gaagcttcgg tcatgccgta cgcttcgagg accggcgcgc cgaacacgga
    29341 ttccatccgt tgcgcggctt ccaccggcag cggcgcgctg cacgaacgga tgaagcgcag
    29401 gaattcggcg cccgccggcc tttcgctgtc tttgccaagg cgggccaaca agagctggtg
    29461 catcgtggga acggccgaat accaggtggc gcgataatcg cgcacggtgc gccagaacga
    29521 gagcggattg aagcgggacg gaaccacgac cgtcccgccc gtaaacagcg tagacatcgt
    29581 cgaagccatc agcccgtgga catgaaacaa gggcatcacc gccagtgaaa tgtcgtccgg
    29641 gcccagttca taggtattgg ctatatttcg gatggaaacg gccaggttgc ggtgtttcag
    29701 cgggacgcgc ttgggacggc ccgtgctccc actggtgtgc aggatcagcg caatgtcttc
    29761 cggggacggc gccgaggcgg agacgccctg cgacggcgat tgcagccgga cagatccgtc
    29821 cgcgcccatt tccacggcca gaataggaat gcggcccccg gccgcacgcc gggcctcctc
    29881 ggcgccttcc gcaggcaaca gcatcacctt ggcattggtg tcttccaggt aaaacgaaaa
    29941 ctcctcttgc cggtaattgg ggttcaaggg agcggctgtg ccggcgatgg aagcggccag
    30001 gaaggatacg atcgccggca atccgtttgg cagagccatc gccactctgt cgccccgctg
    30061 aacgccggcc gccgccagcg catccgccat cgcggcgacc tggctgcgca aagaatcgta
    30121 tgtgatacgg attccggctt cgggaacaat aaccgccgtt cggctgcccg gagctgattg
    30181 cagtatgtcg agaagagtgc ccggtaattt tgcgtcagtc ataagctcgt gataatgcga
    30241 tgctaacaaa aatcgaagcg catggcgcga agaagcgcca aaggcaggcg agccagacgt
    30301 tgcgccccaa aaggttcttc tgcgaaaggc ggcttgcggt gcgtaaggcg ccgaaaattt
    30361 ttcctctggt tcggagactg tggtgtacga tgagacccgt gggtctgacc gccagcacaa
    30421 cgttcgcccc atttgcgcag ggcgacgccg actaatccca atggctcgct ggattcgaac
    30481 ctccgccgac ccggcgctgg tcggttctct cgccaaagaa ttagcgattc ccgaattgat
    30541 atcccggctg ctgattctgc gcggcatcac cggcgcccag caggccgccc gctttctcag
    30601 accgtctctt tcggatcttc acgatcctta ccgaatgctg gggatgcggg ccgccgtcga
    30661 ccggctgcgc cgggcatgcg ccacgggcga gaagatcctc gtctatggcg attacgatgc
    30721 cgatggaacc accgctgtag tgctgctgcg caaagccatc gagctagcgg gaggaacagc
    30781 tgatttctac atacctcacc gcctcagaga tggttacgga atgcgcgacg atgtgatgga
    30841 gcacgcggcg cgccaaggca tccggctgat cgtcagcgtc gataccggca ttcgggaggc
    30901 agtggtggtc gaaaaggcca acggtctcgg catcgacacg atcatcaccg accatcatct
    30961 gcccgaagcc tccccttcgc gggcgcccgc ggcccttgcc gtgttgaacc ccaatcaacc
    31021 gggctgcggc taccccgata agaatcttag cggcgtcggc gtggcattca agctcgccca
    31081 agccctgctc ggttcccttg attggccgcc cgcacggctc gagaaggtac tccgctcgat
    31141 gcttcgaatc gcggccatcg gaaccgtggc cgacgtagtt ccgctagtcg gggaaaacag
    31201 gattatcgtg aaattcggac tgcacgccct cgggcggccg gtgaaccccg ggctgaaggc
    31261 gctgctatcg gcttctggca tagccgaggg ccgcccgccg acggccgcga acatcgctta
    31321 ctggcttgca ccgcggctga atgcggcggg ccgcatggat acggcaagcg aggtgatcga
    31381 gttgttttcg gctgccgatg cggagcgcgg agccaagatc gccgaacgcc tgtgccgcct
    31441 caatgccgaa cgccaggcag ccgaagcacg catcgtagag gagattttga gcgagatctc
    31501 tgcggcgccc gagcgggcat ttccggagca gccgccggct gcgatcgtgg cggctgggga
    31561 aggttggcat cggggcgtcc tgggcatcgt ggcatccaga ctcgtcgagc gcttctatcg
    31621 cccggcagtg gtcatcgcaa tcagcgaaaa agagggcatg gctcacggat cggggcgtag
    31681 catcccgccc tttcaccttc tggaagcgct tgaatccatg gcggaccttt tcgcgcgctt
    31741 cggaggccac cggtccgccg ccggattctc gcttcccgcc gcccggctgc cggaatttcg
    31801 cagccggttc ctgagttatg ctgcggcgcg gctcaatcct gaggattgca ttccgtcaat
    31861 tgccatcgac gctgacctga atttccaaga aattacctgc gagctgctgg gagagcttgt
    31921 tcaactggaa cctcacggcg cgggaaatcc ggagcctatc ttcgcggccg ccggcctcga
    31981 gttgcagcac cctccgcggg tgcttcgcga aaaacatttg cgcctgacgc tgcgccaggc
    32041 aaatcgtagc ttcgccgcaa tgggttggcg gatggcgggg atcgcggcaa gtctgatcga
    32101 gaagactgtt ctcaacgcag cgttcacgtt ggagcgcgac gatggcgggt gccggctgat
    32161 cctcaaagat ttagcgtggc cgtgagtcta gggtccgccg ccgaaagcgc cagatcccca
    32221 attacaggtt ctgccggcgc cgccagccat cccgggggcg cttccgcgcc cccgtaactt
    32281 gatactgaac gctcttccga tgcggcgcga acttaagcgg cgatctcctt gggctcggca
    32341 gcggtaatct gaatttgctt aggtttcatc gcgctggcaa cgggaatccg tatgtcgagc
    32401 agcccgtcgt gcagatgcgc ttggagcttg tcgagatcca ggccagcggg gagatcgaga
    32461 acgcgctcga atttcccata cgggatctgg ttccaaacgt aaccttcttt accgaagttt
    32521 tcaggcgcct tccgctcgcc agaaatgtaa agctgattgc cctgtaccgt gaccttcatg
    32581 tctttttccg tcaccccggg gatcaccacg cgcaggttga gataatcatc ggtccagcca
    32641 gactcgacag ccggcgcgaa gacccagtcg gacgaggcgg tttgaccact ggcccggcgc
    32701 ggtgtcgcat tgaagaagct ctcgaaaagt tggtcgaagg accgccgcat ttcattgatc
    32761 tcatccaggc tacgagtgaa catttcaacc tccttcagaa cctttcgtgt agaaattttt
    32821 gcctttcccg aggcccaagc cctcgcggga ggcctcttca caacgcactt tatcatgtat
    32881 attgagtaat gtcaactaat attttattaa gtatttgcac taatcgttga atcagttcct
    32941 attcacacag ctaagtgggc gaaccgacgt cagcgacggg gctacacggg acgcattcca
    33001 aaaccgcgca ggctccgctc ccacgctggt taagtcccgt atatcaataa attataggaa
    33061 tacaaaagtc agaaggcgcg ggctgcggtt ttggcgaagt acgtgaggat catatcggct
    33121 ccggcgcggc ggattgccgt gagggactcc cacattgcgc ggtccaagtc cagccatccc
    33181 ctttgtgcgg ctgccacgat cattgagtat tccccactca cctggtaggc agcgatgggt
    33241 gcggaaaact gcttgcgggc ctggcagatg acatcgagat agggcatggc cggcttcacc
    33301 ataatgatgt cggctccttc ctcgatgtcg agtgcgatct cccgcagggc ttcgcgtcgg
    33361 ttggctgggt ccatctggta actgcgccgg tcgccgaacc gcggagccga ctcggcggct
    33421 tcgcggaaag gaccatagaa gacggaagcg tacttagcgg cgtaggcgag gatgggcgta
    33481 ttaaagaacc cgtgagcatc aagagtcttg cggatggcgg cgatgcggcc gtccatcatg
    33541 tcggaaggcg ccaccatatc cgcgcccgct tgagcctggg acagcgccgt gcggctcagg
    33601 agctccagcg tcgagtcgtt gtccacatcg ttgccgacga cgcgcccgca gtggccgtgg
    33661 ctggtgtatt cacagaggca aacgtccgcc acgaccagga tgtttttgac ttccctttta
    33721 atggcgcgga tggcccgctg tacaatgccg tcgctcgcgt acgccccgga gccttcggcg
    33781 tctttgcttt ccgggagacc aaacaggatc accgccggaa tgccgagcgc agccacttcc
    33841 ccgcactccc ggacaacctg atcgaccgac atttgatagt tgcccggcat ggcgctgatc
    33901 tcgcgtttga cgccttcgcc cggacacaca aatagcggat aaatgaagtt ggcgggcgtg
    33961 aggcgggttt cctgcaccat cgagcgcagc agtccgcttt gccgcaagcg gcgcagtcga
    34021 acggtgggga atggcatacg ggcattgtaa caagcgcaat cgcggaagtc gggcggcgta
    34081 cttcagcggc ggcgaaccgg aaattcgaaa gagaatctca ggcgtctaca gcgaggcatc
    34141 ggcttcagct tccgccgggc gagcgccggg gaacagcagg cgcagtaagg gcggggcgac
    34201 cagcgtggtt gcaattgcca tgaaaaccac cacggcatac gcatcgcctg aaatcacgcc
    34261 catggtcaat ccgatctggg cgacaaccat gccaacctcg ccgcgcggcg ccatccccat
    34321 gccaacccgg agggcgtcgc gaaaaccgag cgaccacgcc cccaacccgc aaccggccag
    34381 cttagtgatt atggccgccg cagtgataag cagggccaag ccgaatgtac gcaaggactg
    34441 aaagacgcgg atatccaagt acaatccgat ctggaccaga aaaaaaggga caaccagctc
    34501 ggtagctccc tgggccagct ggtggacatc agatccttcg aagtgctcgg ccagaaccat
    34561 gcccgccagg aaagccccga caatggcggc aacgccggcc cacgtcgcca acagcgagag
    34621 accgaaaacc aggaggagag cgaacgcgaa ttcgcctcga atctgccgca gatgggcgag
    34681 ccggggcccg acgcggcgca tggcgcgcgt tcccaccacg gcgacaaata tagtgaagcc
    34741 gaaagccagg gccgccgtgc ccgccagttc cgcatagtgc agcccccctt tggcgacgcc
    34801 gctgacggcg gccagcacga gcaaccccag aatgtcgtcg atcacggcag ccgcaagaat
    34861 gatctggctg gcgcgttcgt tcatgaggcc cagcgaagct agaaccctcg ctgtaatacc
    34921 tacgctagtg gccaccatcg acgcgcccac aaaaatggcc tccacgctgg agtgattcca
    34981 ccaaagcatg atggcccagc cggcaatgaa aggcgcggcg acgccgccgg ccgccaccag
    35041 cagcgcggcg ccccctacct ggaagatctg agccggcttg atctccaggc ccacggtgaa
    35101 gagcaggaaa atgacgccca gttcggccag cgcagagaga aactgatcgg ggttcaccca
    35161 gcccagcacg ctggggccta taac
//
LOCUS       DASREK010000008        40845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1485791, whole genome shotgun sequence.
ACCESSION   DASREK010000008 DASREK010000000
VERSION     DASREK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 40845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40845
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485791"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..3500)
                     /locus_tag="VFA54_00490"
     CDS             complement(15..3500)
                     /locus_tag="VFA54_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS49307.1"
                     /translation="MQNTNRAVRTALLVLWLCAGLADAQTVNGVITGRVIDPGGLAIP
                     GATVVVTREGTGESRQATTVETGDFVFTGMLPGRYSIAVQAPGMKRLEEKSVELTASE
                     RLSVGNLQLQLGTLSDTVTVTAEATPVQTGSAERSAVLTGTQIESLNTRARQYLDLLK
                     VLPGVAYDQPSGLGSVDMLGITQGPKVQGLRGEYNTFQTDGLFMNDLGTKDTLYNPTN
                     MDAVSEVKVLLNNYQAEYGHAGGAIINAVTKSGTRDFHGTGYLYKRHEELNANGFFNN
                     INGIPKPRYRYTTGGGSIGGPLYWPGKINSNRDKLFFFYSNETLRGDAPQPLVQVTVP
                     TALERAGNFSQSLDQNGKLIVIKDPSNGQAFPNNIIPQNRIDSNGQKILSIFPLPNQL
                     NTAITKGAYNYNFQESIFSPKQHNLFRVDLVATSSIRLYFRGAIWNETNIGARLGGGE
                     PTPAWGFLPVAAHYNDNSGVLAFTQTISPTLVHEASVASHHATEASPPASQAVLSTAL
                     RSTYGINLPQFYPQFNPLGVVPWALFGGVPNAANIQTDARFPKRGADTVFVFNDSWSK
                     TWGVHTFKAGFYGERIREYEGQQGTNFGQFDFSRDPNNPLDSNYAYSNAILGNFDSYS
                     ESNARIGDQRRGATFEWYVQDNWKVTKKLTLDYGIRFTHLIPDWSNDGLAWNFNPAQY
                     NPAQAVTLFQPAMIAGGTRVALNPLTGQTLPAVYIGAIVPNSGSLTNGEVPEFGSANR
                     IGKTFQNTAFLQYGPRFGIAYDPFGNGKTAIRAGGGIFYNNRARPGSLNRNPPSEATP
                     FLYYGNLNTYINSSAVLFPSALTAISGTGEVPSVYSYSLGIQRDIGFQTVVDVAYVGN
                     VGRHLQMEQNINAVPYGARFLSKNVDATTGKPLQDNFLRPYTGFSDILFVQNATTSNY
                     NSLQVQVNHRFTRGLQFGVSWTWSKAMDYADTDEGNTSLVSDFLPLRRWNYGKAGFDH
                     THIFVVNWVYQLPKASRLADNRFVRFAFDNWQISNISTFQSGSPYGITLTTVDNTDIA
                     GGGYAPADAPRVIITGNPIIGKDQRTVSRFFNPTVFARPPVGNVGNAPRDVVRGPGIN
                     NWDTSLFKDFPVKERFRFTFRWELYNTFNHTQFFSMDTTARFDASGNQTNARFGALIQ
                     NRDPRRMQGSLRLVF"
     gene            complement(3854..4915)
                     /locus_tag="VFA54_00495"
     CDS             complement(3854..4915)
                     /locus_tag="VFA54_00495"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49308.1"
                     /translation="MKMRHTTGRILLALGLFSSVLSAQTPPRYQVDPAWPKELPDDWV
                     FGRLGGVCVDAHDHIAVVDRRDITKEEAETSHPAPPIVMFDAEGHVIQSIGDPNVVPA
                     SIHGCFFDKENNIWVGGNDDGIIQKYSHAGKLLLQIGKRGVFDTSDGTRRGKSLNASH
                     QQFFLPAGVAIDPENGDIYVADGYGNRRIAVFDKTGNFLRQWGRQATGEEARAGTPGA
                     FAQLVHCVAIGNDGLVYVCDRQGDRVQVFDKAGNFKRNIWIRTGTPNLPDPRGTVWWI
                     AFSIDPEQKFMYVMNGRNEQVHILDHETGKILASFGRPGHQIGNFIHGHTIAFDSKGN
                     LYVAETDWGRRVQKFKVVP"
     gene            5308..6021
                     /locus_tag="VFA54_00500"
     CDS             5308..6021
                     /locus_tag="VFA54_00500"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HZS49309.1"
                     /translation="MFRHTVNTEQDLPSIFLNSDIPAWFAIKVRSRQEKITAKVLSGK
                     GFEVFLPLFRCKRRWSDRIKQVDLPLFSTYLFCRFRLQEQISVLSTPGILQIVSHGKR
                     PEPVEETEISRIRKMIHSGLALEPWRALAIGQAVRLEDGPLKGMTGTLIEVRGSDRLI
                     VSVPLLQRSVAVEIDRQWAYPLQLQRKIRALEFSPASSRTAVYNRAAGKSATGDLSRL
                     ISGQWAYRSDEILHHTNRR"
     gene            5945..7405
                     /locus_tag="VFA54_00505"
     CDS             5945..7405
                     /locus_tag="VFA54_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS49310.1"
                     /translation="MIFLVLFQGNGLIDPMKSYTILIAGDQAAFGGDIQTSFRQMGFA
                     VRSAQNLAEALDVVRATASEIGGVILNLRSAEEGAEAVRQIRRLDERLPVIIAAEESW
                     RPGAGDGLNDAAAVHVRPPFSSASFLAALEQAMKKGSASRQGPWAFRMVCVNPRMRKI
                     RETLEKVALSPAPVVFRGESGAGKEVLARALHDCSPRANKPFVKLNCAALPSELLESE
                     LFGYERGAFTGAFRTTQGKFELADGGSLLLDEIGDMDLKLQAKLLHVLQDWEFYRLGG
                     KKPVQVDVRIIAATHRNLKQAVHDGRFREDLYYRISVVDIEIPPLRRRPEEIRPLLAH
                     FLEKHALPGQKLPAITPELERALLAHDWPGNVRELENVARRLLVFEDSALVAAELAPL
                     RPSKAEISAERFPASLVPPGSARTLGELDRARRKTEADVILAALDSVDWNRRKAARLL
                     QIDYKGLLYKMRKLGISKNLKNSRPPLEHLPTGLVV"
     gene            7443..8741
                     /locus_tag="VFA54_00510"
     CDS             7443..8741
                     /locus_tag="VFA54_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49311.1"
                     /translation="MKKTTILILFLGAISMAQGQEQVYWKPLIPTRGAFPHLGDIEGA
                     PRLRAYSFADGVYESDRETGLPSRQTAEGTELGIGASGYKRWESTALGLDYLSTFRYY
                     SPKIPENGGDHQLTLGLSRTLSRNWKLNVAQSGGIFHRQYLYNPFSALGESFSGAPGS
                     GVFDDRTYYSASAAGLTYQRSPRLAFEISGRGYVVRRTAVGLAGLNSFEGDAGVVYGV
                     SEHNVLRLDYSASHYTYTDVAGEANLQGLAAGWARRLTRNWEIQLRGGAYRAGRLTLT
                     RESVDPSLEPIVPPAPGIRTSATVRFIPLYDAALYRRFRHATAMVGYTRGVDPGDGVY
                     LLSRREGVQFGYNDAGFGRLHWGLIGGYGRYSSLTETAVSRAWWGGGGVTFRVVQSLH
                     LMARYDANRFVVPDKDFTRDRFRITFGIAFAPREVALAMP"
     gene            complement(8689..10107)
                     /locus_tag="VFA54_00515"
     CDS             complement(8689..10107)
                     /locus_tag="VFA54_00515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49312.1"
                     /translation="MRDWISQVPNPKLLLISYVFPPAGGVAVLRALSLAKYLPGFGNE
                     VHVLTAANPAAPVQDPGLLRHVPPQVRVHRCFTPELPFYFRKKLWSWFAGGRNPAGAG
                     RQRRPATGDPESGLGALVKKILCPDPQVLWVPFAQRRAAQIVKKYGIDAVIVTAPPFS
                     VFLTGAALKRRFPHIQLVSDFRDEWLRFYLADFEFLNNDYTRRRAEAIERETIECSDL
                     VVAVTGSSLSEIRKRYPQQPDAKFMLLPNGFDPEVFSGFTPRPHGASKIVMAYLGTVY
                     KTSSPASYLDALDGLPEEVRSCFETRFVGRIAESENATMERRRSPIRLLGFLPQEQAI
                     RQAEEADYLLLIMNNDFMLPGKLFDYLATGKPILAIAPPGGEIGRILKETGAGWCVDP
                     GDRAGIQAALLRAYTQARTGGGGLLRPDAQAIRRYERKRLAGKLDAAIRSLLRDSGRI
                     ETAQGAMASPEPLPWAQMQSRR"
     gene            complement(10058..11272)
                     /locus_tag="VFA54_00520"
     CDS             complement(10058..11272)
                     /locus_tag="VFA54_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HZS49313.1"
                     /translation="MEVLFLSHCTPNPPDKGEKIRAFHAIAHLAARRHVHLVCFARNQ
                     DDIAAARELADRCASVHVEPLRSRGSLAGAILQFAAGRSLTTSFYRKRRLREHVEAIA
                     QRRPVGAVVAYSSAMVPYAPAGVPLLLDMVDVDSQKWFEYARLRRPGFPYRWEGSRLS
                     RLEAQFTRRARCTFVSTHQEAAALRALAPGAAVQVLENGVDFSYFDPLHSPRLPQLHG
                     RRFLVFTGAMDYYPNADAARWFATRIFPELRRGDPGLEFLIVGRHPAHSVRKLASIPG
                     VVVTGTVPDVRPYLVQAQSVVAPLRIARGVQNKVLEALAMRKRVFASTAVCRTFAPEL
                     PEGIVPCHSERDFVSAIGACGWPEPCRESKIRDAAQVRFSWATNMRLLADELEQVAGL
                     DLTGAESQAAAD"
     gene            complement(11364..12410)
                     /locus_tag="VFA54_00525"
     CDS             complement(11364..12410)
                     /locus_tag="VFA54_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HZS49314.1"
                     /translation="MHKPMNIQVRPFAAADSQAWDEFVFRHRHGSPFHLIAWKKSIEE
                     TFGYQPLYLAAEDQNGICGVLPVFLVKNLLVRKALISSPFAVYGGILANCEAAREALK
                     NRLRQLGESLDVEYIELRNAYPEQCVGFSTVCRYVTFTQEIGPDEAAILENIPRKTRY
                     MVRKSLRAGFTTRRQTCDFLAFESLYAENLRRLGTPSFPLKYFERLIANFGRSIDIRE
                     VLLNGKVVSAVMSFHFGDQLLPYYGAADPRYNALAPSTYMYFDQMRWARQNGYRIFDF
                     GRSKKESGSFDFKAHWGMEMRDLPYEILLIRRKELPHYSPQNPRFRAAIRLWQSLPLP
                     VTKALGPSLIRLVP"
     gene            complement(12417..13256)
                     /locus_tag="VFA54_00530"
     CDS             complement(12417..13256)
                     /locus_tag="VFA54_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HZS49315.1"
                     /translation="MLNIVSVDVEEYFHPTEVHALVDMHRWASLPPRVEEATKRVLDV
                     FAEHGVAGTFFILGWVARRHPKLVREIARAGHEIGSHGYAHRLVYELTPAQFREDTAL
                     AQQAIADACGIAPRVYRATSFSITARSFWAFEILAESGFTHDSSIYPVLHDRYGAPGF
                     DRHATLVRTPSGTICEVPIATARIGRNRVAPVGGGAYLRLLPYRYVAAGIRRINEFEG
                     RPACLYFHPWELDPGQPRFRCGWISRLRMYGRIGKMESKVRRLLSDFRFSRLTDVHPG
                     IRV"
     gene            complement(13234..13359)
                     /locus_tag="VFA54_00535"
     CDS             complement(13234..13359)
                     /locus_tag="VFA54_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49316.1"
                     /translation="MSRKINFLDAAVLAFFSSLFGAAVFLLRQIRHSSHAEHRQR"
     gene            complement(13343..14446)
                     /locus_tag="VFA54_00540"
     CDS             complement(13343..14446)
                     /locus_tag="VFA54_00540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS49317.1"
                     /translation="MPVPHIVHVMPSFSPGGAQARTVQIMNYLGESFCHTVVSLNGDF
                     TAAGAVSGDVDVQYLRCRRGGNPIYMIRQFAQLLRALKANLALTYNWGAIDAVVAARI
                     AGGLPVIHSEDGFGADEAIRQKHRRILFRTMTLRSAYRVVAPSETLVDIMRRIWRLPG
                     ERIEHIPNGIDLSRFAPETRRPEQEKVILGAVGVLRPEKNHELLLSACAAAARRTPIR
                     LVIAGDGPERKALEEKAQSLGLAAAVEFLGHCGNPAEIYRRLDLFVLSSKTEQMPLAV
                     LEAMASGLPVVSTDVGDVKKMVSPENRPFIASGEKDFYEALTSLAANPALRKNIGVAN
                     RARCIALYDIRKMLQRYSALYENAIRSSYVAQN"
     gene            complement(14503..15519)
                     /locus_tag="VFA54_00545"
     CDS             complement(14503..15519)
                     /locus_tag="VFA54_00545"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZS49318.1"
                     /translation="MNILITGGAGFIGSHVAERLLRKGRRLTIIDDLNDFYPPAWKRQ
                     NLAEITQTGPIDFHQIDICDQKAVFTVVARAMPDVIIHLAARAGVRPSLEDPLLYEQV
                     NVRGTLVLLEASRRFGVRKFIFASSSSVYGAENRVPFRETGCVDFPISPYAATKIAGE
                     RLCYTYSHLYSISVVCLRFFTVYGPRQRPDLAIRKFVESIEQGRRIQVFGSGASARDY
                     TYVEDIAAGVAAAVSYHCGYELFNLGNSDPITLTDMIRTIENALGKKAILDHVPEQPG
                     DVPITYADISKARRLLDYRPATKFPEGIEKFVAWHRRRPAFCARRQKGSKSETRAPNF
                     PAVW"
     gene            complement(15516..16820)
                     /locus_tag="VFA54_00550"
     CDS             complement(15516..16820)
                     /locus_tag="VFA54_00550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49319.1"
                     /translation="MISPSETEQLSESGPAALPGRPQALASALASKIKILSLCCVYPN
                     PSEPSLGLFVRSRLQHLGELADVKVIAPLAAFDYSRWRAVRSIRRSVPKDRTDGNLEV
                     LQPAWFYPPLGGWLNPLFLFSRLRRLAVHLRRDFPFHIIDAHFGYPDGIAAALLARCT
                     RSPFSVTLRGNELMHARRKLRRAALGWALRRADAIIAVSESLRRFAISLGAEPEKVKT
                     IPNGVESRIYFPRNRVQCRVKLGLPAGGRFVLSAGALIERKGHHRVMEALKSLAEEGC
                     TACLLIAGGPGREGSFVKELHQLPAKLNMQRRVRFFGEVSPERMAELMSAADVLCLAS
                     SREGWPNVVHEAMACGTPVVASNVGGVPEMIPSDHFGFVVPPGDPVALAAALRSALEN
                     SWDRAAIAAWAHSRSWQQVGREVLWTLCQAVAAGRHAEEQQR"
     gene            complement(16817..18268)
                     /locus_tag="VFA54_00555"
     CDS             complement(16817..18268)
                     /locus_tag="VFA54_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HZS49320.1"
                     /translation="MAKSSFLYLLGNVASRAVGFLMIPVYARCLRPDEYGLIELLELC
                     TQVAAICIGLQSVGGAMSRIYFEYSDRRDRAAVVSSAVLSTAGLGTALAVLAVTAAAP
                     LSLLTLGSRQYASLTATAFVAMSLATLGEVVLVYYRMLDKAVFFVGYSLVQLVVSLTL
                     NIYFIKFQGMGVWGFVYTKLITGASGTLLLSCLLFRETGIHWRWEPVKKMAIFGYPLI
                     VSSLAMFIIHFSDRFFLEKLGGLTAVGVYALAYKFAFLVTYLVGEPFAKVWYATVYSY
                     TRQDGWLDDFLRIGRYLVFCLFLAGLAVALFINQAMAIVASPAFAAAAGLVPVLALGY
                     VLRGMGDFYRSLLYVHMRSAQASQIAAFCGFVNLALNYWLIQNYLAFGAAYATLLTWA
                     AYLGCCWSMSRKQYQIAFPLKSFALVFCTAAGVYWCSRWQPDLPAVFQWGRSALLLVM
                     FVGLLWMFRYFPLRERELIYCRLARLRCKLTSE"
     gene            complement(18392..19240)
                     /locus_tag="VFA54_00560"
     CDS             complement(18392..19240)
                     /locus_tag="VFA54_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS49321.1"
                     /translation="MTPFRPRLLKLSRQITRLVAPELKNSQFAYYEMVRQVLDCKPQP
                     RTDQGPEEFTWLDIGCGHHIFGHWMDREEREAAGKCPSIFGLDASFEALQKHRAIRNR
                     TLGDILHAPFRGSSFDLVTANMVFEHLERPFESLLEIGTLLKPGGTLVLHTPNRRHYL
                     VFIAALLPRSARQRIACFLDGRAKEDVFPTLYRANTPKRVRLLAQTAGFEVLEIRLVN
                     SSVVTGMLGPLVVVELLISRLLSLKKLRNFRSNMIVLLRKAECYGPAAEAATDAMHGI
                     ATCSHG"
     gene            complement(19237..20610)
                     /locus_tag="VFA54_00565"
     CDS             complement(19237..20610)
                     /locus_tag="VFA54_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HZS49322.1"
                     /translation="MREILLIFFVAALSAAALIKPQVGLCGYIWFSIMRPDAWAWSSG
                     LPYSMALAVATLAGSLRYLPAVTALFKSPISVLLLVLQIPVLLSAVFAQDPSLCWEPL
                     NLYARVIVMALLVAVLVRTKQQLHNTLLLISVFLGALGARFGLYGILNGGVQFSSGNA
                     GFMSDNNDLALGLAMGVPLCWYSRALVRPVWAKAAFLLMLFGSIAAIIMTHSRGAALS
                     LAAVFALIAVNERRRMAVLLLLLLMAAPSIHLVRQTYLPRLATLEAPEEENSALLRLI
                     YARGAVRMWLDHPFLGVGFGSRNQMLLWHKYMSEATRSEPQVIHNTYLQILVDSGFFA
                     LLIYAVLLLGTIIFLQKSAARAKWEHPGMEAYPLALAASLAAFAVGCTFLSRVEFDLA
                     YILLMCAASWQAIQKRLAAEKPAAGPQQPEAAGRPGPIRAASQARWACTAAKTRLLAD
                     TGKHSLA"
     gene            complement(20702..23038)
                     /locus_tag="VFA54_00570"
     CDS             complement(20702..23038)
                     /locus_tag="VFA54_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49323.1"
                     /translation="MKPVLIVLLPMMLAVAAPAAGPSNAITISDLGGNSGKRRPFTIS
                     RFFARGDIRHFAAARIDGTVAMTQCDVKTRWPDGSVQHALVSFFAPVGRDETLTVDFV
                     DQPAGNNDHPLGSAEMLRLDYWGAEMEAAAAGVAKSADARAMLRAGSWRYWLRGPLCT
                     QVIVEGRRDGPSSAFAYDFGWRWDGKNMALSDDPKFRSLHPIFVLTFYRNWPGAKIEC
                     ILENDWTTKLQDQRYSLKIYEGNPAPRVVYRNPAVIHLAKTRWRKVFWSGEQPPPVSV
                     DYNLPYLIYSRVLPNYDLTRVVPASAIAEEAAAFQRTDRGDICGRGQWEPVFPRPGGR
                     PDIGLFPRWYVRYLFTFDPQLYEVMLGDAAVSGHVPIHYREAAAGRPFRRGSQTGAFG
                     RVLSIDARPTVRTTYQTQDTRPEDAITPAGPATAGPWVVDLAHQASFAFIPYLITGDW
                     YYLEELHFWAAYDLARGNPGVNVSYGRHAAWGFINDGEEIRGVAWGLRNLAHAALAAP
                     DGSPEKAYFTEKLRNNIAIREGKYNITGGEFYRPCQTYPYDGRRETSMWCWGRQTAEE
                     NRNNPLHFLDGGSPGLRQGLDESKVYAGSSPWMWNYNQIVLGYLNELGFPVRKLHETL
                     AKNLINQLQNPDYNPYLAAAYRIPVRKQQGAYIGTWAEVRRGYANPGITGFRASDSRD
                     VEHGYGRIVQAAASFLPGIVDGRLTGKGAWEWINTNLGEADLLNNNPKWALVPRRTGP
                     ELAVQAAERSFRAAEIRRQRQIRLAGPAALKPSPLCSN"
     gene            complement(23035..23799)
                     /locus_tag="VFA54_00575"
     CDS             complement(23035..23799)
                     /locus_tag="VFA54_00575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS49324.1"
                     /translation="MHFDLAEYRAGRREQTRVRDLMAILPQGRHSVLDIGARDGYISR
                     LLASRFQRVTALDLEKPAFTLENVKTVKGDVTKLDFPDNSFDVIVCTEVLEHIAPRLL
                     RQACREIVRVVRHEAVIGVPYKQDLRAGRTTCQTCKKKNPPWGHVNSFDERVLVKLFY
                     PLRVARLSILGPPARVTNFVSAFLMDLAGNPWGTYNQHEPCVYCGAPLAGPVPRSPAQ
                     RLCSAAAVLLEEIQNTFHRSSPTWIHMVFQKNGTRP"
     gene            complement(23799..25004)
                     /locus_tag="VFA54_00580"
     CDS             complement(23799..25004)
                     /locus_tag="VFA54_00580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49325.1"
                     /translation="MVILAYYFPPENAIGADRPFRFAKYLPRFGFVPRVIANNAEECR
                     EMDSVTRVPRRERHKPGLALAAGAARAVQKLLPYNEQLAWAPHAVAAGSRAIHKESAK
                     LVLSTSPPLATHLAALRLKQRFGVRWIADFRDPLVGNPCRTRRFGKIYDSALERCIFS
                     HADLLIANTESAAEAWRKRYPQWAGKIRTIWNGFDPEDAVRPSVIPRRPQALLAHVGS
                     IYGLRDPRPVLWTIERSTALGLLNPGALQVLFAGPLEERIFSLTDPPFSTLAAKGCLR
                     CDNRLLSRSDARRLIATADYLLLIDHASAETIHVPAKLFEYIQVGRPILALTSRNSPC
                     DQILAKAGVPYKCMYMDASPEQACARLQEFLSLPNRAVPASRWFWEQFDGSRQTETLA
                     SLLRCLLAS"
     gene            complement(25094..27046)
                     /gene="asnB"
                     /locus_tag="VFA54_00585"
     CDS             complement(25094..27046)
                     /gene="asnB"
                     /locus_tag="VFA54_00585"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZS49326.1"
                     /translation="MKGRSYTFLGKPDMCGITGYLACNSAAPDRSIVERMCERLSHRG
                     PDSSGFFCDQHAALGHRRLSIIDRRGGNQPLSNEDGSIQVVFNGEIYNFQALRRKLIE
                     KGHKFATNSDTEVLVHLYEDAGERMPELLNGMFAFAIWDQKNQQMFLARDRLGEKPLY
                     YSFSVPGVRFCFASELKALTAVPGFDAPVSGRSVCDFLAFSYIPDPQTIYRNVFKLEP
                     GSSLTVNCASYHIRRYWEPAFLADRRLRFDDAVEALRDLAVDSVKCRLISEAPLGAFL
                     SGGVDSSAVAGILARETGQRPKTFSIGFTSPKFDERAFARLMAGRCHTEHHEYVVSPE
                     AHDAIEAVTRHFDEPFGDPSAIPTLHLARVTRQDVTVALGGDGADELFGGYRRYLFGV
                     IEDRLRAAWPGWFRRSVIGLGAQYYPKFDYLPRIFRAKTLLANLSQEIGHAYFTSMSV
                     FRDRELDCVLSSGLRRELRSYSPRDSFSARFAAVRHLSPLEQMQAVDLETYLPGDILV
                     KLDRATMAHSLEARSPWLDYRLAELAFRLPSHFKIRGTRGKVVFKEAVAPFVPREILT
                     RRKMGFSPPLAAWFRSSLAPAFRALVFRPEMEEYVNLPEVRRLWDEHQSRTWNHDRKL
                     WNLLMLAGWDVHRQKSPVAELVEAAV"
     gene            complement(27145..27942)
                     /locus_tag="VFA54_00590"
     CDS             complement(27145..27942)
                     /locus_tag="VFA54_00590"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HZS49327.1"
                     /translation="MESIFGNLTPLPDPPTDLGPGLHGLHSAPARGDHSSPYRTVSLQ
                     IKSRLPLLRFGDDHWRSAEQYRIIRTKLTQHPRQPKLIGISSAAPGDGKTVNAANIAA
                     AISLKQQAKVLLADADFRRANLAWALGVRSGPGLAEVLAGDCSVGTAIVRLENLPNLH
                     FLPAGENRNAPAELLDSPAWREVCQRVRQDFQFVIFDAPPIGLVADYDLLQAALDGIV
                     LIVRPGHTNRTQLSTALQTVSPDKLLGVVVNCVQESLFWKRHDYYYY"
     gene            complement(27951..29627)
                     /locus_tag="VFA54_00595"
     CDS             complement(27951..29627)
                     /locus_tag="VFA54_00595"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HZS49328.1"
                     /translation="MTDRPDFSPVSLLHTLWTRKFSILAIWALVTAGVIAAVRRLPAV
                     YKAEAVVLVESQRIPEKYVPSTVNADLQERLNTISQQILSSSQLWQIIEKFNLYPEAR
                     KTLSQDEIVQRMRSDIGISIDSRWRKDRPGAFRISYIGSVPTVVSQVANYISGLFIDE
                     NIRTRESQAEGTSQFLEDQLAQAKARLEEQESKLSEYKMKYNGELPQQENTLAAALGQ
                     FQMQLQGLQESISRTEQQKLMLENAIESARTSLDALSQTSAAAAPESLGPNSASPAAP
                     AEKRSDRLRAELDALRMRYTEDHPDIKLAKAELARELRQEAARAAAASPPAAASPQEK
                     TSSAADLSAKLQMEQTLNRERERVENLEVQLKATAKDLDSLEAERRRVVQSISAYQAR
                     IERLPIREQQLAAVSRDYEISKATYRSLLDKKLAANVAADMERRQQPERFTMLEAAKA
                     PERPLKPDRLLLSALGSVLGLFLGVTFAVGAQVRKNVLLGEWELPAGVWVLGRVPTID
                     PNIADRPRTNRQRAEDGRGRQPKTRWVLAFSTILLMLGAMAAAGIYFGWV"
     gene            complement(29874..31259)
                     /locus_tag="VFA54_00600"
     CDS             complement(29874..31259)
                     /locus_tag="VFA54_00600"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZS49329.1"
                     /translation="MIQIFNQYTSLKLLALMMIEALLIFISILFSGRFLDVWNQGEPP
                     VRFCLEALGLLILVQTAFYCNNLYDFSQRRPRRLELLYILQSIAAAVVIVGLASPLIP
                     GRILGDRVLAGAFLLTVGSVCLVRISVNKVWPNVLVSQNVAILGSGELAEAVARELLA
                     RRDLNARLVGFVGNVTRPMLGRPVLGGYEDLSAIASRHQISRIIVAMEDWRGALPTSE
                     LMKVRVSGVAVEDAHSALAALTGRVWLVAVRPSWLSLFVMSGGFRRSRLRGGIKRATD
                     ICFGVLGLIVTLPLQLAVALAVLIQSGRPVIYRQVRVGLRGKCFELLKFRSMRVDAEM
                     DGRAQWAQRDDPRITRVGRFIRKYRLDELPQFINVIRGDMSFVGPRPERPYFVEQLKS
                     KLSYYELRHMVRPGITGWAQVRHNYTASLEDSLRKLEYDLFYLKNMSFLFDCSIVFQS
                     IRTVLLGLGGP"
     gene            31330..32070
                     /locus_tag="VFA54_00605"
     CDS             31330..32070
                     /locus_tag="VFA54_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZS49330.1"
                     /translation="MKPWTASSISPRIRENTGLRCVVRCLITLVFLAVAGAQTPGHVV
                     QPAGKSAKMIVEARGAPGAAAKVENRPVTVTPEPSKLGLRAGYRIGPGDVLEINVWKE
                     PQLSTGAAVVRSDGRISLPLVKEVDALGLTPGELERILSEKFSQFLEVPEVTVIVKEV
                     HSEKVYVIGGVKKPGSVDLYSQLTVLQVLAEAGGLTEYAKKGGIFVLRKNGDKQVRLH
                     FDYNAQLKGKPGKPEIVLQPGDTVVVPQ"
     gene            complement(32003..34414)
                     /locus_tag="VFA54_00610"
     CDS             complement(32003..34414)
                     /locus_tag="VFA54_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49331.1"
                     /translation="MKNASVFGTHVRSLLVLFATAAGTLGAAPSNSITITDLSGLPKT
                     RQPFTISRIFAQGEIPDFAQAEVDGALVVTQCDVKTRWPDGSLQHAMLSFVTEIGERK
                     TATVAFVNQSDGNNKPFLAKDDLFDPAAPWASYLGAVVTATQSSSHTADLRTMLANWD
                     GREDDLGVRYWLKGPVVSQLIVEDKNPATVAYDFGWDCTSGDSPCSGGWSDSADAGGK
                     SLHPIFVVSVYRGFPSVRIEAILENAWSTKLKDQTYSLEIRLGNPPAAAAAYSNPAVN
                     HIAKTRWRKVFWSGPQPYPVRIDYNLPYLIHSQAIPSYDLTKVVGPADVLSETVSFRS
                     GDQGNIGGHGQWMQAFPTTGGRGDIGLFPRWYVRYLYTFDPDLYEVVLGDAAVSGYVP
                     IHYRESDLGRRFLKSQPEDAGGRVLSIDARPTFRSTAQTQDTRAEDLVVPIGPTSNGK
                     WIVDLAHQPSFAYIPYLITGDWYFLEEIYFWASFDVAHGNPQVSLSYDRHADWGFIND
                     SEEIRGVAWGMRNLAHAAFAAPDGALEKAYFTEKLYSNIAIREGKYNITDGSFYEPCH
                     TSPYNALKETSKWCWGRNTVAGNTDNPLHFLEEGSSGLTTGLDTKQVYAGSSPWMLNY
                     NHIVLGHLKELGFPVAKLQETVAKNLLNQILNPDYDPFLVASYRIPVLRQPDRRFLPD
                     WGSVKAGFLDPSPAGFSETDMADAEHGYTHIAQAAASFLPGINDGPLRGQDAWDWMTS
                     HVGRQSLLNSNPKWALVPRSGPAAACRPEVLGRRNFALQPKLTAGPQPYRRVAAQSPA
                     SQACP"
     gene            35182..36813
                     /locus_tag="VFA54_00615"
     CDS             35182..36813
                     /locus_tag="VFA54_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZS49332.1"
                     /translation="MNFEDVAAAAGLNFRNVAGDRTEKLYILEATGGGVAIFDYDNDG
                     WPDIFLVNGSKFNFAADEERPVSRLFRNLGAHADPPGLRFEDVTEKAGLSRSGWGQGA
                     CVGDYDNDGFEDLFVTYYGANVLYHNNGDGTFRDVTAAAGLPTSGTRWSTGCSFFDYD
                     RDGRLDIAVANYLQFDPSQVPKPGETPFCVYKGLPVMCGPRGLPAGVNILYHNEGGGK
                     FRDVSEQSGFSKPEGRYSFAVLAADFDNDGWPDVYIACDSTPSILLHNNHDGTFTDIG
                     VLSGAAFNEDGQEQAGMGAAAGDYNNDGWLDIVKTNFSDDTPTLYLNNKDGTFTDVTY
                     AAGLGVNNRFLGWGVGFVDIDNDGWKDIIMVNGHVYPSIDSLKLTSPYREEKNVYWNL
                     RNGAFLDISARAGPAILDRRAARGAAFGDLNNDGALEVVINNIDDAPSLLVNRGEKKN
                     WLLVRTQGTKSNRDGIGARVTVTAGSLQMSAEVGSGSSFLSHNDLRLHYGLGNSKQSD
                     NIRVRWPSGLEEVFPGSPANKEVLLVEGKGHPAGK"
     gene            37343..40186
                     /locus_tag="VFA54_00620"
     CDS             37343..40186
                     /locus_tag="VFA54_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZS49333.1"
                     /translation="MSKTAGRLIQILVVAILGGFLVRMHTQAQEAAPEQYALPLPIDR
                     GAASVWQSLKKLHTRASLMMVTAHPDDEDGAVLAYESRGVGTRVVLLTLTRGETGANV
                     MSPDYFDALGLVRTQELLAADRYYGAQQYFTRVIDYGFSKTKEEALEQWSHDRVLADV
                     VRIVRMTRPLVITSVFVGGITDGHGNHQVAGQMAQEVFLAAGDPSKFPDQIQAGLRPW
                     KPLKVYARVPFVGRMSEKGLYDYATHTWHPARLFDYVRQQWLPVPLPANVEVPVGDYD
                     PVLGLNYSQISREGLGHQKSQNGGTGMPPAGAATRAYHRFGSIVPASEKETSFFDGID
                     TSLTGIAGLAPSGDTAFLKRGLGEINRIVEQAMAEFNAAHPESIAPRLAAGLKTTAAL
                     IDQVKASSLPADAKQDIVYELEVKRTQFNEAIAQALGISLLATVAPEKEPAGMAAMFG
                     MTPESFQVAIPGQQFYVKVHVVNQSKNPVELKDVSLRGPEGEDWAIERQGAAGGALAN
                     NQPSDIRFRVHVPGNAHFTRPYFTRPNIEQPYYDIQDPKYLGQPLPPYPLAARVELNY
                     EGQPIEVSQVVQTVKRVTGAGDVLQPLVVGPAVSVSISPSAGVVPLDAKSFQVSATVH
                     SNVKGPAQGVVRLDLPAGWRCSPERAPFATSKDGEDQSVSFEVTPANLRPQPYKIAAL
                     AILAGNEYREGYHMAGYQGLLPYNLYHPAVYKTTGVDVKIAKGLNVGYVEGSGDEVPQ
                     SLENLGVQVHYLTPLEIATGDLHKYDVILLGVRTYAVREDLRTYNGRLLDYVKNGGVV
                     VVQYNTPEFDHNYGPYPYTMTNDPEEVTDEQSKVEILDPSNPVFTWPNLITEDDFKGW
                     VEERGSKFMSSWDPHYEALLSTNDPGQAPQKGGLLYARYGQGVYIYNAYAFYRQLPEG
                     VPGAYRIFANLVSLPRHPQLRAASAAPSRTAGR"
     gene            40188..>40845
                     /locus_tag="VFA54_00625"
     CDS             40188..>40845
                     /locus_tag="VFA54_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS49334.1"
                     /translation="MQNPGERKGAPAGQPGLIRGMGLPQATAANMLNMIGIGPFITIP
                     LILSTMGGPQAMLGWVLGLLLAICDGLVWAELGAAMPGAGGSYIYLREAFGPRTWGGL
                     MSFLFIWMIVFIAPLSAASAAVGFSNYLKYLWPGMTHTQAVLVAVAVCGAVTALLYRN
                     IESVGWLSVVMLWVVVLTTLWIIGAGLTHFNAARVFSFPPGAFRLDRAFFQSLGAATL
                     I"
BASE COUNT         8549 a        12065 c        12299 g         7932 t
ORIGIN      
        1 gccagcacgt gcgatcaaaa caccagccgc agcgaaccct gcatccggcg aggatcgcgg
       61 ttttggatca aggcgccaaa ccgggcgtta gtctggttgc cgctggcgtc gaaacgggcc
      121 gtagtatcca tgctaaagaa ctgcgtgtgg ttgaacgtat tgtacagttc ccagcggaaa
      181 gtaaaccgga accgctcctt caccggaaag tctttgaaca gggatgtgtc ccagttgttg
      241 atgccgggtc cgcgaaccac gtcccgcggc gcgttgccta catttcccac cggagggcgg
      301 gcaaagacgg tggggttgaa gaagcggctg accgtcctct gatctttgcc gatgatcgga
      361 ttgccggtta taatcacccg cggcgcatca gccggagcgt agccgccgcc ggcgatatcg
      421 gtgttgtcga cggtcgtaag tgttatcccg taaggcgacc cgctttgaaa cgtggagatg
      481 ttggagattt gccagttatc gaacgcgaat cgcacaaagc ggttgtcggc cagccggctc
      541 gccttgggaa gctggtagac ccaattgacc acgaatatgt gcgtgtggtc gaacccggcc
      601 ttgccgtagt tccaccggcg gagcggcagg aaatcggaga ccagggacgt gttgccttca
      661 tcggtgtcgg cgtaatccat agccttcgac caggtccagc ttacgccgaa ctgcaacccc
      721 cgggtaaagc gatggttcac ctgcacctgg agggagttgt agttcgaggt ggtggcattc
      781 tgcacgaaaa ggatgtcgct gaagcccgtg taggggcgca gaaaattatc ctggagcggc
      841 ttgcctgtag tagcgtcgac atttttgctt agaaaccggg ccccgtaggg aaccgcgttg
      901 atgttctgct ccatctgcaa gtgccgccct acgttgccta cgtaggccac atccaccacg
      961 gtctggaagc cgatatcccg ctggatgccc aagctgtaac tgtagacgct gggaacttct
     1021 ccggtgccgg agatggcggt caacgcagaa gggaacagga cggcgctgct gttaatgtac
     1081 gtgttcaggt ttccatagta aagaaagggc gtcgcctccg agggcgggtt gcggttgagc
     1141 gatcccggcc gcgctcgatt gttgtagaag atgcccccgc ccgcgcggat tgctgtcttg
     1201 ccgttgccga acgggtcata ggcgatcccg aaacgcggcc cgtattgtag aaacgccgtg
     1261 ttttgaaaag ttttcccgat gcgattggcg gatccgaact ccggcacttc gccgttggtc
     1321 aggctgccgg agttcggaac gatggcgccg atgtataccg caggcagcgt ctggcccgtc
     1381 aagggattca gcgcgacgcg cgttcccccg gcaatcatcg ccggctggaa aagcgttaca
     1441 gcctgcgctg ggttgtattg cgccgggttg aaattccagg ccagcccgtc attggaccag
     1501 tcaggaatca gatgggtgaa acggatgccg tagtcaagag tgagtttttt ggtcaccttc
     1561 cagttgtcct gcacatacca ctcgaaggtg gcgccgcgcc gctgatcgcc aatgcgcgcg
     1621 ttcgattcgg aataggaatc gaaattgccc aaaatggcgt tggaataggc gtaattcgaa
     1681 tccagcggat tgttcgggtc gcggctgaag tcgaattggc cgaagttggt gccctgctgg
     1741 ccttcgtact cgcggatgcg ctctccgtag aaaccggctt tgaaggtgtg aacgccccag
     1801 gttttgctcc aactgtcatt gaacacgaag actgtgtctg cgccgcgctt gggaaaccgc
     1861 gcatcggtct ggatatttgc agcgttggga acccctccga aaagcgccca gggcacgact
     1921 cccagcggat tgaattgcgg gtaaaattgg ggcaagttga tgccgtacgt ggaccgaagc
     1981 gcagtgctca acacagcctg actggccggc ggagaggcct cggtggcgtg gtgcgaggct
     2041 acagaggcct cgtgcaccag cgtagggctg atcgtttgag taaaggctaa aacaccggag
     2101 ttgtcgttgt aatgagcagc cacgggaaga aagccccagg ccggagtagg ctcaccgcct
     2161 ccgaggcggg cgccgatgtt tgtctcattc cagatcgctc cgcggaagta tagccggatg
     2221 ctgctggtgg ccaccagatc cacccgaaac agattgtgct gcttcgggct gaagatgctc
     2281 tcctgaaagt tgtagttgta ggcgcctttc gtgatcgccg tgttgagctg gttgggcagc
     2341 gggaaaatgc tcaaaatctt ctgcccgttg ctgtcgatgc ggttctgagg aatgatattg
     2401 ttcgggaatg cctggccatt ggacggatcc ttaatgacga tcagctttcc gttctgatcc
     2461 aacgactgcg agaaattgcc ggctctttcg agcgccgtag ggacggtcac ctgcacgagc
     2521 ggctgaggtg catcgccgcg cagggtttcg ttgctgtaaa agaaaaacaa cttgtcgcgg
     2581 ttggagttga ttttccctgg ccagtagagc ggcccgccga tcgagccccc gccggtcgta
     2641 taacggtatc ggggctttgg gattccgttg atgttattga aaaatccgtt ggcgttcagc
     2701 tcttcgtggc gcttgtaaag atagcccgtg ccgtggaaat cgcgcgtgcc ggacttagta
     2761 accgcgttga taatcgctcc gccggcatgg ccgtactcgg cctgatagtt gttgagcagc
     2821 acctttactt cggaaacggc gtccatgttc gtcgggttgt acagcgtatc cttggtgccg
     2881 agatcgttca tgaagagacc gtcggtctgg aaggtgttgt actcgcctcg caacccttgc
     2941 actttagggc cttgggtgat cccgagcatg tctaccgatc caagcccgct cggctgatca
     3001 taggccacac ccggcaggac cttgagcaaa tccagatact gccgcgcccg cgtattgagg
     3061 ctctcgatct gagtgccggt gaggacggcc gatcgctcgg cgctgccggt ctgcaccggc
     3121 gtcgcctcag ccgtgactgt gacggtgtcg ctgagcgttc ctagctggag ttgcaggttc
     3181 cctacagaca gccgctcgga agccgtcagc tcgacgctct tctcctccag acgcttcatg
     3241 ccaggcgcct gtacggcgat cgagtagcgc ccgggcagca tccccgtaaa aacaaagtcg
     3301 ccggtctcca ccgtggttgc ctggcggctt tctccggttc cctcgcgtgt gacaactacc
     3361 gtcgcccccg gaatcgccag ccctcccgga tcgatgacgc gcccggtaat caccccgttg
     3421 acggtctgtg cgtctgctag gccagcgcat agccaaagaa cgagcagagc cgtgcgtaca
     3481 gcacgattgg tattttgcat ccggcgcccc ccaacaaaga aagatcatgc cgtatgaggc
     3541 tggctggagt ggatgcgaaa ccgggaaccc cggcccggaa cggaagcgat gctgcaaccg
     3601 ggacagcaaa actgaaacct ggcagcacag aagacgcacg cagaaccggt tcgttagccg
     3661 ctccagccat agagcgcggc ggaggcccgt tggggtcgat tttggatgga atcggctatt
     3721 ctccccggcc tcgccagact tgcatgaaag cataatcgcc cacccaagtg ttgccgtgag
     3781 tttagtgcgg aaccagcggc ctgtcaagcg agattggctg cgacggccga gagggaataa
     3841 acctacccgt gcttcaggga acgaccttga atttttgcac gcgccggccc caatcggtct
     3901 ccgctacata aagattgcct ttggagtcga aagcgatggt gtggccgtgt atgaaattac
     3961 cgatctgatg gccgggccgt ccgaagcttg cgagaatctt ccctgtttcg tggtccagaa
     4021 tgtgcacctg ctcgttgcgg ccgttcatca catacatgaa tttctgctcg ggatcgatcg
     4081 agaaggcaat ccaccagacc gtcccgcgcg ggtcgggcag attcggagtt cccgttctga
     4141 tccaaatatt ccgtttgaag tttcccgcct tgtcgaacac ctgcacccgg tcgccctgcc
     4201 ggtcgcaaac atagaccaac ccgtcattgc cgatagcgac gcagtggacc aactgcgcaa
     4261 atgcgccagg agtcccggcc cgggcttcct cccccgtagc ttgccggccc cattggcgca
     4321 ggaagtttcc cgttttgtcg aacaccgcta tccggcgatt gccatagccg tcagccacat
     4381 agatatcccc attctccggg tcgatggcga cgccggcggg aaggaaaaac tgctggtggc
     4441 tggcattcaa ggacttgcct ctccgcgtgc cgtccgaggt gtcgaaaacg ccgcgcttgc
     4501 cgatctgcaa cagaagcttt cctgcgtggc tgtacttctg aataatgccg tcgtcgttgc
     4561 ccccaaccca aatattgttt tccttgtcga agaagcaccc gtggattgag gccggcacga
     4621 cgttcggatc gccgatggat tggattacgt ggccctcggc gtcaaacatt acgatgggag
     4681 gcgcgggatg cgacgtctct gcttcttctt tcgtgatgtc ccgccggtct accacggcaa
     4741 tatgatcatg cgcgtccacg catactccgc ccaggcgtcc aaacacccaa tcatcaggca
     4801 gctccttagg ccaggcgggg tccacctggt agcgcggcgg agtttgcgcc gataaaaccg
     4861 aactaaagag gccgagcgcc agcagaattc ttcccgttgt gtgtctcatt ttcatatttc
     4921 gcgaagatcc cacgacaagg ctgccgggat ccaccgcatc ccatccttgc attggctttt
     4981 gcccatccgc agcagtatac accgccaccg gcccgaggcg atgccaagca ggccggttcg
     5041 tctttgccaa cgcgccggcg caaaggggct tcgctcaaat ctctcggtcg tatgaccatg
     5101 ctgttctgaa actggaacag tcgccaacat cggccagcga gcgaaggctt ctcgattttg
     5161 tgatccgcgc ctttcgttgg cttttagaaa gtgaacatat ttctgattta ttaaggaaaa
     5221 ccgcagaagg atctacgcgt atttactaat gtactccggt cactcagcgc ggcatcatat
     5281 acgtttggaa agaaaaaagc cgggcccatg tttcgtcata cagtcaatac ggagcaggat
     5341 ctaccgtcca ttttcctaaa cagcgacatt cccgcgtggt ttgccatcaa agtcagatcc
     5401 cgccaggaaa aaatcactgc gaaggttcta agcggaaaag gctttgaggt gtttttgcca
     5461 cttttccgat gtaagcggcg ctggtccgac cggattaagc aagtggatct tccgctcttt
     5521 tctacatact tattttgccg tttccgcctg caagagcaga tatcagttct ttcaacgccc
     5581 ggcattctgc aaattgtgag ccacggaaag cggcctgagc cggtggaaga aacagaaatt
     5641 tccagaatcc gaaaaatgat ccattccgga ctggcgctcg agccatggcg cgcgctggcg
     5701 atcgggcagg ccgtccgcct ggaagacggc ccgctgaaag gaatgacggg gactctcatt
     5761 gaagtgcggg gttccgatcg gctgattgtt tctgtaccgc tgctccagcg ctcggtcgca
     5821 gtcgagattg accgtcaatg ggcttacccg ttgcagctac aacgcaaaat cagggccctc
     5881 gaattttctc cagcgtcttc ccgcacggcc gtatataacc gagcggccgg aaaatccgcg
     5941 accggtgatc tttctcgtct tatttcaggg caatgggctt atcgatccga tgaaatctta
     6001 caccatacta atcgccggtg atcaggctgc attcggcggc gatatacaga cctctttccg
     6061 gcaaatggga tttgccgtgc gcagtgcgca gaacttggcc gaagctttgg atgtcgttcg
     6121 cgctacggct tctgagatcg gcggagtcat actgaacctg cgaagtgcgg aggaaggagc
     6181 ggaggcggtc cggcaaattc gccggctgga cgagcggctc ccggtgatta tcgctgcgga
     6241 ggagagttgg cggccagggg ctggggatgg tcttaacgac gccgctgccg tccatgtgcg
     6301 cccgcctttt tcttcagctt cttttttggc ggctttggaa caggccatga aaaaaggaag
     6361 cgcctcccgg cagggaccct gggcgttccg catggtttgc gtgaatccgc ggatgaggaa
     6421 gatacgcgaa actttggaaa aggtggccct gtctccagcg ccggtagtct ttcggggcga
     6481 gagcggcgcc ggcaaagagg ttttggctcg ggctttgcac gattgctccc cacgcgccaa
     6541 taagcccttc gtaaaactga actgcgctgc cctgccttcc gagctgttgg agagcgagct
     6601 gttcggctat gagcgtggcg cattcaccgg ggcttttcga accactcagg gcaaatttga
     6661 gctggcggac ggcggcagct tgctgctcga cgagatcggc gatatggatt tgaagctcca
     6721 ggcaaagctc ctgcacgtgc ttcaggactg ggagttctac cggctcggcg gaaagaagcc
     6781 cgtccaggta gacgtccgga ttatcgccgc gacgcaccgc aatttgaagc aggcggttca
     6841 cgacggccgc ttccgagaag acctgtatta ccggatcagc gtggtggaca tcgaaattcc
     6901 tccgctgcgg cgccgtccgg aagaaatccg gcccttgctg gcgcattttc tggaaaaaca
     6961 cgccctcccc gggcagaaac tgcctgccat tacgccggag cttgagcggg cgctgctcgc
     7021 acacgactgg ccgggaaacg tccgggaatt ggagaatgtg gcgcgccgcc tgctggtctt
     7081 cgaggattcc gctttggtgg cggcggaact ggcgccgttg cggccctcca aggccgagat
     7141 ttccgcggag aggtttccag cttcgcttgt gccacccggc tctgcgcgca ccttgggtga
     7201 attggatcgc gccaggcgaa aaactgaggc agacgtcatc cttgcagcgc tcgacagtgt
     7261 ggactggaac cggcggaagg ccgccagatt gctgcaaatc gactacaagg ggttgcttta
     7321 caagatgaga aagctgggaa tcagcaagaa tctgaaaaat tcacgtccgc cgttggagca
     7381 cctgccgaca gggcttgttg tgtgacgggc tcgtgaagca gccgcggatg cagagtactg
     7441 cgatgaaaaa aacaaccatt ctcattctat ttttgggcgc catctcgatg gcgcaaggcc
     7501 aggaacaagt ttactggaag ccgctgattc ccactcgcgg cgcgtttccg cacctgggcg
     7561 acatcgaggg cgcaccccgc ctgcgcgctt actcattcgc ggatggcgta tatgaaagcg
     7621 accgggaaac cggactgccc tcccggcaga ccgccgaagg cacggaactg ggaatcggcg
     7681 cctcggggta caagcgctgg gaatccaccg ccctgggcct cgactacctg agcacctttc
     7741 gctattactc gccgaaaatt ccggaaaacg gcggggatca tcagctcacc ttgggccttt
     7801 cgcgtacgct ttcgaggaac tggaagctga atgtggcgca atcgggcggc atttttcacc
     7861 ggcagtatct atacaacccg ttcagcgcat tgggcgaatc gttttccggc gcgcccggca
     7921 gcggcgtctt tgacgaccgg acatattact ccgcctccgc cgcgggcctc acgtatcaga
     7981 gaagcccgcg tctcgctttc gagatcagcg gccgaggata cgttgtgcgg aggactgccg
     8041 tgggcctggc gggtctgaat tccttcgaag gcgacgctgg tgtggtgtac ggcgtttccg
     8101 aacacaacgt cctgcgcctc gattacagcg cttcgcacta cacttacacg gatgtggccg
     8161 gcgaagcgaa tttacaggga ctggccgcag gttgggcgcg ccggttgacg aggaactggg
     8221 aaattcaatt gcggggcgga gcctaccgcg ccggccgtct gactttgacg cgcgagtcgg
     8281 tggatccctc gctcgagcct atcgttcccc cggctccagg catacgaacc agcgctacag
     8341 tccggtttat acctctatac gacgcggctc tctataggcg gttccgccac gcaacggcca
     8401 tggttggcta tacgcgagga gtggatccag gcgacggagt ttacttgtta tcgcggcgcg
     8461 agggggtgca gttcggctac aacgacgcgg gtttcggacg cctgcactgg ggattgatag
     8521 gcggatacgg gcggtacagc agtcttacag aaacggctgt atcgcgagcg tggtggggcg
     8581 gcggcggggt gactttccga gtcgtacagt ccttgcacct gatggcgcgc tatgatgcga
     8641 atcggtttgt cgtgcctgat aaagatttca ccagagaccg cttccgtatc accttcggga
     8701 ttgcatttgc gcccagggaa gtggctctgg cgatgccata gcgccttgcg ccgtctcgat
     8761 ccttcccgaa tccctgagca ggcttcggat tgcggcgtcc agctttcccg ccaagcgttt
     8821 tcgctcatac cgccggatcg cctgagcgtc gggccgcagc agcccgccgc ccccggtgcg
     8881 agcttgggta tacgctcgca gcagcgcggc ttgaatgccg gcgcggtcgc cgggatccac
     8941 gcaccagccg gctccggttt ctttcagaat tcttccgatc tcccctcccg gaggagcgat
     9001 cgccagaata ggctttccag tggccaaata atcgaaaagc ttcccgggaa gcatgaaatc
     9061 gttgttcatt atcagcagca gataatccgc ctcctcggcc tggcggatcg cttgttcctg
     9121 aggcaagaaa ccgagcaggc ggatcgggga acgccgtcgc tccatcgtgg cgttctccga
     9181 ttcggcaatc cgcccgacaa agcgggtctc aaagcatgag cggacctctt ccggcaatcc
     9241 atcgagcgca tccagatagg aagcgggcga agatgttttg tataccgtcc ccagataagc
     9301 cattacgatc ttagaagcgc cgtgagggcg cggcgtaaat cccgagaaaa cctccggatc
     9361 gaagccgttg gggagcagca tgaatttggc gtccggctgc tgcgggtatc gctttcgaat
     9421 ttcgctcagc gatgagccgg tgacggctac cacgaggtcg gaacactcta tggtttctcg
     9481 ctcgatggct tcggcacggc gccgcgtgta gtcgttgttc agaaattcga agtcagccag
     9541 ataaaatcgt agccattcat cgcggaaatc gctgaccaat tgaatatgcg ggaatcggcg
     9601 tttgagtgcg gcgcccgtaa ggaacacgga gaagggcggg gcggtcacga ttacggcgtc
     9661 aatgccgtac tttttgacga tctgtgcggc ccggcgctgg gcgaaaggga cccacaacac
     9721 ttgcggatcg gggcacaaaa tctttttcac caacgcaccg aggccggatt ccgggtcccc
     9781 ggttgccgga cgcctttgcc ggccggcgcc cgccgggttc cggccgccgg caaaccagct
     9841 ccatagcttc ttccgaaaat aaaacggcag ctcgggcgtg aagcagcggt gcacccggac
     9901 ttgaggcggc acatgacgca gcaagccggg gtcttggacc ggagcggccg gattggccgc
     9961 tgtcagcacg tggacttcat ttccaaagcc cggcaaatac tttgcgaggc tcagggcccg
    10021 cagcacagcc acgccgccgg ccggagggaa aacgtagcta atcagcagca gcttgggatt
    10081 cggcacctgt gagatccagt cccgcaactt gttccaattc gtcggcgagc agtctcatgt
    10141 tcgtggccca cgaaaatctg acctgggcgg cgtcgcggat tttggattcc cggcagggtt
    10201 ccggccagcc gcaagcgcca atagcgctga caaaatcacg ctccgagtgg cacgggacga
    10261 tgccttcggg aagttccggc gcaaaagtcc tgcacaccgc tgtagaggca aagacccgct
    10321 tgcgcatggc taacgcttcc agcaccttgt tctgaactcc ccgcgcgatg cgcaaaggag
    10381 cgacgacgga ctgcgcctgg accagatacg ggcgcacgtc gggcaccgta ccggtgacga
    10441 ccacgcctgg aatcgaggca agcttccgca cgctatgcgc cggatggcgg cccacgatca
    10501 gaaattccaa gcctggatcg ccccggcgca gttccggaaa gattcgagtg gcaaaccaac
    10561 gggcagcgtc agcgttcgga tagtaatcca tcgctcctgt gaaaaccaga aatctgcggc
    10621 cgtgaagctg cggcaaccgg ggtgaatgta aaggatcgaa ataggaaaaa tccaccccat
    10681 tttccaggac ctgaaccgcc gcgccgggcg ccagagcccg cagcgccgcc gcctcctggt
    10741 gcgtggatac aaacgtacac cgggcccgcc gcgtgaactg cgcctccagg cggctgagac
    10801 gcgatccttc ccaacggtag ggaaaacccg gacgccgcag gcgggcgtat tcaaaccact
    10861 tttgcgaatc cacgtccacc atatcgagca gcagcggcac gcccgcaggc gcatagggaa
    10921 ccattgccga agagtaagcg acgaccgccc ccaccggacg cctttgcgca atcgcttcca
    10981 cgtgttctcg caaccgcctt tttctataga acgaagtcgt gagggagcgg ccggcggcaa
    11041 actgtaggat ggcgccggcc aaagaacctc ttgagcgcag cggttcgaca tgcaccgagg
    11101 cgcagcggtc ggcaagctct cgggccgctg cgatgtcgtc ctggttgcgc gcgaagcata
    11161 caaggtgcac atggcgccgg gccgccagat gcgcgattgc gtggaaggcg cgaatttttt
    11221 cccccttatc cggaggattc ggcgtgcaat gcgacaggaa aaggacctcc atggagtgaa
    11281 tctggtaggc ggggcaggaa tcgaacctgc ggcctcctcc gagtgataga ggcgctctac
    11341 cgctgagcta cccgcctgaa cttttagggg acgagccgta tcaaggaggg tcccaatgct
    11401 ttcgtgaccg gcaagggcag gctttgccag agccggatgg ctgcccggaa acgtggattt
    11461 tgggggctgt agtgcggcag ttccttgcgc cggatcagca aaatctcata gggaagatct
    11521 ctcatttcca ttccccagtg cgccttgaaa tcaaaagatc cgctctcttt cttgctgcgg
    11581 ccgaaatcaa aaattcgata gccgttctgc ctggcccacc gcatctggtc gaagtacata
    11641 taggtgctcg gggcgagtgc gttgtagcga ggatcggctg cgccgtaata aggcagcagc
    11701 tgatctccga aatggaaact catgacggcg gaaacgactt tgccgttgag caagacctcc
    11761 cggatgtcaa tgctccggcc gaagttcgcg atcagccgct cgaaatattt gaggggaaag
    11821 ctcggggttc ccagccgtct caggttctcg gcatagaggc tctcgaaggc caggaaatcg
    11881 caggtctgcc ggcgcgtcgt aaaaccggcc cgcagcgatt tgcggaccat gtaccgggtt
    11941 ttgcgcggga tgttttccag aatggccgcc tcgtcagggc cgatttcttg cgtgaaggtc
    12001 acatagcggc agacagtgga aaacccaacg cactgctcgg gataagcgtt gcgcagctcg
    12061 atgtactcca catccaatga ctcgccaagc tgccggagac gatttttgag agcctcgcgc
    12121 gccgcttcgc agttggcgag aattccccca tagactgcga acggcgaaga aatgagagcc
    12181 ttcctcacca gcagattctt caccaagaaa accggcagca cgccgcagat gccgttttga
    12241 tcttcggcag ccaaataaag cggctgatag ccgaaggtct cctcgatgct ttttttccag
    12301 gcgatcaagt gaaaagggct gccatgccgg tggcgaaata cgaattcatc ccacgcttgg
    12361 gaatctgccg ctgcaaaggg gcgcacttgg atattcatag gtttgtgcat cggttctcaa
    12421 acgcgaatcc cgggatggac atctgtcagc cgggagaaac gaaaatccga gagcaagcgc
    12481 cgcacctttg actccatttt gccgatccgg ccatacatgc gcagtcgtga aatccagccg
    12541 cagcggaacc gcggctggcc gggatcgagt tcccagggat gaaagtacag gcaggccggg
    12601 cggccttcga actcattgat gcgccggatt cccgccgcaa catagcgata cggcagcaag
    12661 cgcagataag ccccgccgcc caccggggcc actctatttc tgccgatgcg agctgttgcg
    12721 atgggcacct cgcaaatggt acctgaagga gtgcgcacca gcgtggcgtg gcggtcaaat
    12781 cctggagcgc catagcggtc gtgaaggacc ggatagatgc tggaatcgtg ggtgaagccg
    12841 gattcggcca ggatttcgaa cgcccagaac gaccgggcgg tgatggaaaa gctggtggcg
    12901 cgataaacgc gcggcgcaat gccgcaggcg tcggcgatgg cctgctgcgc cagggccgta
    12961 tcttcccgaa attgagccgg cgtcagctcg tacacgagcc tgtgggcgta gccgtgagag
    13021 ccgatttcgt gtccggctct ggcgatttcg cgcaccagct tggggtggcg ccgggccacc
    13081 caaccgagaa taaaaaacgt cccggcgact ccgtgttccg cgaaaacgtc gagcacgcgc
    13141 ttcgttgcct cttccacgcg tggcggcagc gaggcccacc ggtgcatgtc cactagagca
    13201 tgcacctcgg tgggatgaaa gtattcttcg acatcaacgc tgacgatgtt cagcatggga
    13261 actgtggcgg atctgcctca acaggaacac cgcggcgccg aacaggctgg aaaaaaaagc
    13321 cagaaccgca gcgtcgagga agttaatttt gcgcgacata gctgctccgg atagcgttct
    13381 catatagtgc ggaataacgt tgcagcatct ttcggatgtc atagagagcg atgcagcggg
    13441 cccggttcgc cacgccgatg ttttttcgca gggccggatt cgcggccagg ctggtgaggg
    13501 cctcgtaaaa gtctttctcc cctgaggcga tgaagggccg gttttccggg ctgaccatct
    13561 tctttacatc tcctacgtcg gtgctgacca ccgggagacc ggaagccatg gcttccaaca
    13621 cagccagcgg catctgctcg gttttcgagc tcaacacaaa cagatccagg cgccgataga
    13681 tctcggctgg attgccgcag tgccccagaa actccactgc ggccgccaag cccagggatt
    13741 gcgccttctc ctccagggcc tttcgttccg gtccgtcgcc cgctatgacg agccgtatgg
    13801 gcgtacggcg ggcagccgcc gcgcaggcag agagcagaag ttcatggttc ttttcaggcc
    13861 gcaagacgcc gacagcgccc aagatcacct tttcttgctc cggacggcgg gtctcagggg
    13921 caaatcgact caaatcgatc ccgttcggga tgtgctcgat cctctcgcca ggaagccgcc
    13981 agatgcggcg catgatgtca accagagttt ccgaaggagc caccacgcga taggcggaac
    14041 ggagagtcat cgtacggaac aaaatgcgtc ggtgcttctg gcggatcgcc tcatcggctc
    14101 caaatccgtc ctcggagtga atcacaggaa ggcctccggc gatgcgggcg gcgaccacgg
    14161 cgtctatggc gccccagttg taggtaagcg cgagattcgc cttcagcgct cgaagtagtt
    14221 gcgcgaactg gcgaatcata taaatcggat tcccgcctcg ccggcatcga aggtattgca
    14281 catcaacgtc cccgctgacc gccccggcag ccgtgaagtc cccgttcaaa gacacgacgg
    14341 tatgacaaaa cgactctccg agatagttca tgatttggac cgtgcgcgcc tgcgccccgc
    14401 cgggagaaaa actgggcatc acgtggacaa tatgtggaac cggcatggcg atctcttcga
    14461 tttgtcgatt ggccttgcgg gagagccgcg cggggaatcg catcaccaga cagcaggaaa
    14521 atttggcgcc cgcgtttcgc tctttgatcc tttttgccgc cgggcgcaga acgccggccg
    14581 gcggcgatgc catgcaacga atttttcaat gccctccgga aatttcgtgg ccggccggta
    14641 atcgagcaat cggcgagcct tggaaatgtc ggcgtaggtg atggggacgt cgcccggctg
    14701 ttccggaaca tgatcgagaa tagccttttt gccgagcgcg ttctcgatgg tccgaatcat
    14761 gtccgtaagg gtgatgggat cagaattgcc gagattgaac agctcatagc cgcagtggta
    14821 ggaaacggcc gccgcgacgc cggcggcaat gtcctcgaca tacgtgtagt cccgcgcgct
    14881 cgcgccgctg ccaaagacct gaatcctgcg tccctgctcg atggactcga cgaatttccg
    14941 aatcgccaga tcgggcctct gccgcgggcc gtaaacggtg aagaagcgca ggcacactac
    15001 agagatcgag taaagatgcg aataggtata gcagagcctt tcgcccgcga ttttggtagc
    15061 ggcgtaggga gagatcggga agtctacgca gcccgtttcc cggaaaggca ctcggttttc
    15121 cgccccatat acggaactgg aggaagcaaa gataaatttc cggacgccga agcggcggct
    15181 ggcctccagc aggaccagcg tgccccggac attgacttgt tcgtagagca ggggatcctc
    15241 aagcgagggc cgcactccgg cccgcgccgc caaatgaatg atgacgtcgg gcattgcccg
    15301 ggccaccacc gtgaagacag ctttttggtc gcaaatgtct atctgatgga aatcgatggg
    15361 gccggtctgg gtgatttccg ccaggttctg ccgcttccat gcgggtgggt aaaaatcgtt
    15421 taggtcatcg ataatggtaa gccgccggcc ttttctaagg aggcgctcgg cgacatgaga
    15481 accgatgaag ccggccccgc ccgtgatcag gatattcatc gctgctgttc ctcagcgtgc
    15541 cggcccgcgg caacggcttg acacagggtc cataaaactt cgcgtcccac ctgttgccac
    15601 gagcgggagt gcgcccaggc ggcgatggca gctcggtccc acgaattttc gagcgcgctc
    15661 ctgagggctg ccgcgagggc gacagggtcg ccgggtggaa cgacaaaacc aaaatgatcg
    15721 gaaggaatca tctccgggac tccacccacg ttcgaggcca ccaccggcgt tccgcaggcc
    15781 atcgcttcat gcactacatt cggccagcct tcgcgcgaac tcgccagaca cagaacgtcg
    15841 gcggccgaca tcagctccgc catcctctct ggagacactt caccgaaaaa gcggactctt
    15901 cgctgcatat tgagctttgc cgggagctgg tgaagctctt tcacaaaact cccttccctg
    15961 cctgggccgc ctgcgatcag caaacacgca gtgcagcctt cctccgccag gctcttcaga
    16021 gcttccatca cacgatggtg ccctttgcgc tcaatcaagg ctccggcgga aagcacaaaa
    16081 cggccgccgg caggcaaccc gagtttcacc cggcattgca cccggttcct ggggaaataa
    16141 atacggcttt caacgccatt cggaatggtc ttgacctttt ccggctcagc gcccagtgaa
    16201 atggcaaaac gccgcaggct ttcggaaacc gctatgatgg cgtcggcgcg gcgcagcgcc
    16261 cagcccagcg cggcgcgccg cagcttacgg cgcgcgtgca tcagttcatt gccgcggagt
    16321 gtgacggaaa aaggggaccg cgtgcagcgc gccagcaaag cggcggcgat gccatcggga
    16381 tatccgaaat gggcgtcgat gatgtgaaaa ggaaagtctc ttctcaagtg caccgccaga
    16441 cggcgcagcc gggaaaaaag aaacaacgga ttcagccacc ctcctagggg cgggtagaac
    16501 caggcgggct gtagaacctc gaggtttcca tcggttctgt ctttgggaac cgagcggcgg
    16561 atactgcgaa ccgctctcca ccgggagtaa tcaaaggcgg caagcggagc gataactttg
    16621 acatcggcca gttcgcccaa atgctgcaag cgagaccgca cgaacaaacc aagagacggc
    16681 tccgacggat tcggatatac gcaacaaagg ctgagaatct taatcttcga cgccagggcc
    16741 gaagcaaggg cttgcgggcg gcccggcaga gccgccggcc cggattccga aagctgctcc
    16801 gtttcagatg gggaaatcat tcgctggtca gcttgcagcg caggcgcgcg agacggcaat
    16861 aaatcagttc ccgctcgcgc aacggaaaat accgaaacat ccacagcagc cccacaaaca
    16921 ttacgaggag aagggcgctc cggccccact ggaaaacggc ggggagatcc ggctgccagc
    16981 ggctgcacca atacacgccg gccgcggtgc agaacacgag cgcaaacgac tttaggggaa
    17041 acgcaatctg atactgtttg cggctcatcg accagcagca tccgagatag gccgcccaag
    17101 tcagcagcgt cgcataagcc gcgccgaaag caaggtaatt ctgaatcagc cagtaattga
    17161 gggccagatt tacaaacccg cagaacgcgg cgatttgact cgcctgcgcg gagcgcatgt
    17221 ggacgtaaag caagctgcgg tagaaatcgc ccatgccccg aagcacatac cccagagcca
    17281 gaaccggaac caggcccgca gcggcggcaa aagccggcga agctacaatg gccatcgcct
    17341 gattgatgaa cagggccacc gcgagaccgg ccagaaacaa acaaaaaacc agataccgcc
    17401 cgatgcgcag aaagtcatcg agccatccgt cttgccgggt atatgaatat acagtggcat
    17461 accagacctt ggcgaacggc tcgccaacca gataagtgac caggaaagcg aacttgtagg
    17521 ccagcgcgta gacgcccacg gccgtcaatc cgccgagttt ctccagaaaa aagcggtcgg
    17581 aaaaatggat gataaacatc gccaaagacg acacaatcag cggatatccg aaaatcgcca
    17641 ttttttttac cggctcccag cgccaatgga ttcctgtttc ccggaagagc aaacagctca
    17701 atagcaaagt gcccgaagcg cccgtaatca gtttcgtgta cacgaagccc cacacgccca
    17761 ttccttgaaa cttgatgaaa tagatattga gcgtgagact gacaacgagc tggaccagag
    17821 agtagccgac aaagaacacc gctttgtcca acatgcggta atagacgagc acaacctcgc
    17881 cgagagtggc gagagacatt gccacaaaag ccgtggcggt caaagacgca tactgccgcg
    17941 agccgagagt gagcaggctt aaaggtgcgg cggcagtcac tgccaagacc gccagcgccg
    18001 ttcccaaccc ggcggtgctt agaacggcgg aagaaactac ggcggcgcgg tcgcggcgat
    18061 cggaatattc aaagtaaatc cggctcatgg cgcctcccac cgactgcaat ccgatgcaaa
    18121 tggccgctac ttgcgtgcac aactcgagaa gctcgatcag accgtactcg tcgggcctca
    18181 ggcatcgggc gtaaaccggt atcatgagga agcccaccgc ccggcttgcc acgttgccaa
    18241 gaagatacag aaatgacgat ttggccaaac tcttcagatt catatccaag ggggccgaat
    18301 tacgatgttc aggtccgagt tgccgggcag ggtggacgtc cagactatgg ggcctatatg
    18361 catattccac tgcaaagggc catgcgcggc attagccgtg cgagcaggtg gcgattccgt
    18421 gcatcgcatc cgtcgccgcc tccgcagcag gcccgtagca ttccgctttt cgcaagagga
    18481 cgatcatgtt gcttctgaaa tttcttagct tcttgaggct taacagccgg gaaatcagca
    18541 gttcaaccac tacaagcggc cccagcatgc cggtcaccac ggaggagttc accagcctga
    18601 tctccaatac ttcaaagccg gcggtctgag ccagcaggcg cacgcgcttc ggagtgttcg
    18661 cccggtaaag cgtcgggaag acatcttcct tcgcgcgccc atcgaggaag caggcaatcc
    18721 tttgcctggc ggatcgcggc agcagcgccg caataaagac caggtagtgc cgccgattcg
    18781 gcgtgtgaag caccaacgtg ccgccgggct tcaacaaggt cccaatctct agcagggact
    18841 cgaagggccg ctccaaatgt tcaaacacca tgttggccgt taccaaatca aacgacgacc
    18901 cccggaacgg cgcatggagg atgtcgccca gcgttctgtt ccgaatcgcc cgatgcttct
    18961 gcagggcttc gaagctggcg tccaggccaa aaatggaagg gcatttaccg gcggcctccc
    19021 tctcctcgcg gtccatccaa tggccgaaga tatgatgccc gcagccaata tcgagccagg
    19081 taaattcttc cgggccctgg tccgtccgcg gctggggctt gcagtccagc acctgccgaa
    19141 ccatttcgta gtaagcaaac tgcgagtttt tcagttctgg cgccacaagc cgggtgattt
    19201 gccgggagag ctttagcaga cgcgggcgaa acggcgtcat gcgagagaat gctttccagt
    19261 atcggccagt aatcgggttt tggcagccgt acaggcccac cgcgcttggg acgcagcccg
    19321 aataggaccc gggcggccgg ccgcctccgg ttgctgcggc ccggcggccg gcttttccgc
    19381 agcaagccgc ttctggattg cctgccagga cgcggcgcac atcaggagga tgtacgccag
    19441 atcgaactca actcgagata agaaggtgca gcctaccgcg aaggccgcca gagaggctgc
    19501 aagcgccagc ggataagcct ccatgccggg atgctcccat tttgcccgcg cggcggattt
    19561 ctgaagaaaa ataatggtcc cgagcagcag aacggcgtaa atcagcaaag cgaagaagcc
    19621 cgaatccacc agtatttgca gataagtatt atgaattacc tgcggctccg agcgggtagc
    19681 ctcggacata tacttgtgcc acagcagcat ctggttgcgc gagccgaagc cgactcccag
    19741 gaatggatga tcgagccaca tccgcaccgc gcccctggcg tagatcagcc gcagcagggc
    19801 tgagttttct tcctcgggcg cttccagcgt cgcaagcctc ggcaggtacg tttgccggac
    19861 caggtgaatc gaaggcgcgg ccatcagaag aagcaagagc agcacggcca tccttcggcg
    19921 ttcgttaacc gcaatcaggg caaaaactgc cgccagcgaa agggccgctc cgcgggaatg
    19981 agtcatgatg atggccgcga tgcttccaaa taacatcagt aagaaagcag ctttggccca
    20041 caccggccgc accaatgccc gggaatacca gcacagcgga actcccatag ccaggccgag
    20101 ggccaggtcg ttgttgtccg acatgaaacc ggcattgccc gaggagaact ggactccgcc
    20161 attcaggatg ccgtagagtc cgaaccttgc gcccagcgca cccaaaaaca cgctgatcag
    20221 caatagcgtg ttatgcaatt gctgctttgt cctgaccagc actgccacaa gcaacgccat
    20281 cacgatcacc cgggcataaa gattcagcgg ttcccagcag agggacgggt cttgcgcaaa
    20341 aaccgcagac aaaagcaccg ggatctgcaa aaccaaaagg agaaccgaga tcggactttt
    20401 aaatagcgct gtaactgcgg gaagatagcg caaactgccg gcaagcgtcg cgaccgccag
    20461 agccatcgag taggggagac cggacgacca cgcccaagcg tcgggccgca tgatcgaaaa
    20521 ccaaatgtaa ccgcaaaggc cgacctgcgg cttaatcaga gcggcggcgc tcaacgccgc
    20581 cacaaaaaaa atcagcagga tttcgcgcat cgcggtattt aggaaaccga tacgggtttg
    20641 aagaactgct tgccgctatc tggttttcgg gaccgtatgg agttgcagga cgcgctggga
    20701 atcaattact acagagcggt gagggcttca gcgcagcggg gccggccagc cgtatctggc
    20761 gctgtctgcg gatctctgcc gcccggaagc ttctttcggc ggcttgcaca gccagttcgg
    20821 gaccagtcct gcgcgggacc aaagcccatt tgggattatt gttcagcaga tccgcctccc
    20881 ccagattggt attgatccat tcccaggcgc cttttcccgt caaacgccca tctacaatac
    20941 cgggcaaaaa gctggctgcg gcctggacga tccggccata accatgctcg acatcgcgag
    21001 aatcacttgc ccgaaagccg gtgatgccgg gattggcgta accgcgtcga acttcggccc
    21061 aggtgccgat gtaggcgccc tgctgttttc gaaccggaat ccggtacgcc gccgccaggt
    21121 atgggttgta atcggggttt tgaagctgat tgatgagatt tttggccagg gtttcgtgca
    21181 atttgcgcac aggaaaaccg agttcgttga gatatccaag aacgatctgg ttatagttcc
    21241 acatccaggg cgagcttccg gcgtagactt tcgattcgtc aaggccttgg cgcagccccg
    21301 gactccctcc atccaagaaa tgcagcggat tgttgcggtt ctcctccgcg gtttgcctgc
    21361 cccagcacca catggaagtc tcgcgtcggc catcataagg atacgtctga caaggccggt
    21421 agaattcccc gccggtgatg ttgtacttgc cttcccggat cgctatgttg ttgcggagct
    21481 tttctgtgaa ataggccttt tccggtgagc cgtccggggc agccagagcg gcgtgcgcga
    21541 gatttcgtaa gccccaggca acgcccctga tctcctcgcc gtcgttgatg aatccccacg
    21601 cggcgtggcg cccatacgag acgttcactc ccggattgcc gcgcgcaaga tcgtaagctg
    21661 cccagaaatg cagctcttcc aggtaatacc agtcgcccgt gatgagatac ggtataaaag
    21721 caaagctggc ctgatgcgcc agatctacca cccacggccc agccgtcgcc ggaccggccg
    21781 gcgtgatcgc gtcctcgggc cgcgtgtcct gggtctggta cgtggttcgg acagtcggcc
    21841 gcgcatcaat ggaaagcacc cggccaaatg cgccggtctg gctgccgcgc cggaacgggc
    21901 ggccggcggc ggcctcacgg taatggatgg ggacgtgtcc gctcacagcg gcatcgccca
    21961 gcatgacctc atagagctgc ggatcgaagg taaagaggta gcgaacatac cagcgcggaa
    22021 acaggccgat atcgggacgc ccgcccggtc tgggaaaaac gggctcccat tggcctcttc
    22081 cgcaaatatc tccccggtcc gtgcgttgga acgcggccgc ttcctcggca atcgccgaag
    22141 caggcacgac cctggtcaag tcatagttcg gcaacacacg ggagtaaatc aggtacggca
    22201 ggttgtaatc cacgctcacc gggggcggct gctcgccgct ccaaaacacc ttcctccatc
    22261 gggttttcgc cagatggatg accgcgggat tccggtatac gactcttggg gcggggtttc
    22321 cctcgtaaat cttgagggaa taccgctggt cctggagttt agtggtccag tcgttttcca
    22381 ggatgcattc gattttcgcg ccgggccagt ttcgataaaa cgtcagtaca aagatggggt
    22441 ggagcgaccg gaatttcgga tcatccgaga gggccatatt cttgccgtcc cacctccagc
    22501 cgaaatcata ggcaaacgcc gaggatggcc catcccggcg tccttcgacg atcacctgtg
    22561 tgcaaagcgg tccgcgcagc cagtagcgcc aggacccggc gcgcagcatg gctcttgcgt
    22621 cagccgattt cgcgacgccc gccgcagcgg cttccatctc ggctccccaa taatccaggc
    22681 gaagcatctc ggctgaacca aggggatgat cgttgtttcc cgccggctga tccacgaaat
    22741 ccactgtcag ggtctcatct cggccgacag gggcgaaaaa agaaaccaat gcgtgttgta
    22801 cgctgccgtc cggccagcga gtctttacat cgcactgcgt catggcgacg gttccatcaa
    22861 tccgggcggc cgcgaagtgc cggatgtcac cgcgagcgaa gaaccgcgaa atcgtgaagg
    22921 gccggcgttt tccgctgttt ccgcccaggt cgctgatcgt gatcgcattc gatgggccgg
    22981 ccgccggcgc agccacggcg agcatcatgg ggagcaaaac gatgagcacg ggcttcaagg
    23041 ccgcgtgccg tttttttgaa acaccatgtg gatccacgtg ggcgaggagc gatgaaacgt
    23101 attttggatt tcctccagca acacggcggc cgccgaacac aatctctggg ccggagatcg
    23161 cgggaccggg ccggccagcg gcgctccgca gtacacgcaa ggctcgtgct ggttgtaagt
    23221 tccccagggg ttccctgcca ggtccataag aaatgcggac acgaagttcg ttacgcgcgc
    23281 tggagggccc aaaatgctga ggcgggcgac tcggagcgga taaaacaatt tcacaagcac
    23341 gcgttcatcg aaggaattga catgccccca aggcggattt tttttcttgc aggtctggca
    23401 tgtggtcctg cctgcccgta gatcttgctt gtagggaacg ccgatcaccg cctcgtgacg
    23461 gacgacgcgg acaatttcgc ggcaagcctg gcggagcagc ctgggagcaa tatgttccag
    23521 gacttcggtg catacaatca cgtcgaagga attgtccggg aagtcgagct tcgtaacgtc
    23581 gcctttcacg gtcttgacgt tttccagagt aaaggctggc ttctcgaggt ccagggccgt
    23641 tacccgctgg aagcgggagg ccagcaggcg ggagatatac ccatctcgcg cgccgatatc
    23701 gagcacggaa tgcctcccct gcggcaggat ggccatcaag tcgcgcaccc gcgtctgttc
    23761 ccgccgcccc gcgcgatatt ccgcgagatc aaaatgcatc acgaagccag caaacagcgc
    23821 aacagcgaag ccagcgtctc cgtttgccgg cttccatcaa actgctccca gaaccaccgg
    23881 ctggcaggca cagctctgtt gggaagcgac agaaactctt gaagacgcgc gcaggcctgc
    23941 tccgggctcg catccatata catgcatttg taaggcacgc cggccttcgc cagaatctgg
    24001 tcgcaaggag aatttctcga tgtaagagca agaatcggcc ggccgacctg gatatattca
    24061 aacaactttg cgggaacgtg gattgtttct gccgaggcgt gatctatcag cagtaaataa
    24121 tctgcggtgg caatgaggcg cctcgcgtcg cttcggctca aaaggcgatt gtcgcaccga
    24181 aggcatccct tggccgcaag cgtcgaaaag ggaggatcag tgagggaaaa gatgcgttcc
    24241 tccagcgggc cggcgaacag cacctgcaag gcgcccggat ttagcaagcc cagcgctgtg
    24301 gagcgctcga tggtccaaag aacagggcgg gggtctctga ggccatagat gcttcccacg
    24361 tgcgccagca gcgcttgtgg gcggcggggt atgaccgacg gccgaaccgc gtcctcagga
    24421 tcgaagccgt tccagatcgt ccgtattttg ccggcccatt gcggataccg cttgcgccag
    24481 gcttcggccg cgctttctgt gttagcaatc aacaggtccg catggctgaa gatgcagcgc
    24541 tcgagggcgc tatcataaat tttgccgaac ctccgtgtgc ggcaggggtt cccaaccagc
    24601 gggtcgcgga aatccgcgat ccacctcacg ccgaagcgct gcttgagccg caacgccgcc
    24661 aggtgcgtgg ccaaaggagg ggaagtggaa agcaccagct tcgcggactc cttgtggatc
    24721 gcacgggagc ctgcggcaac agcgtgcggc gcccatgcca actgctcgtt gtagggcaga
    24781 agcttttgca cggcgcgcgc cgcgccggcg gcaagcgcca ggccgggttt gtgtctctct
    24841 ctgcgcggga cgcgagtgac agaatccatt tccctgcact cctccgcgtt atttgcaatc
    24901 acgcgaggga caaacccaaa ccgcggcagg tacttcgcga accgaaaagg ccggtcggct
    24961 ccgattgcgt tctctggagg gaaatagtag gcaagtatta ccacagcttc gcggcccgct
    25021 gggatttgca gcggcgcagc ctcgagctgc ggcgtacggc ggcagcccaa gctccgcgag
    25081 atgagcgctt gcttcaaacc gcggcctcca ccaattcggc gaccggtgat ttctgccggt
    25141 gcacatccca accggcgagc atcagcaggt tccagagttt gcggtcatgg ttccaagtgc
    25201 gcgattggtg ctcgtcccaa aggcgccgca cttccggcaa gttcacgtat tcctccattt
    25261 cgggccgaaa cacgagcgcg cgaaatgccg gggccaggct ggagcggaac caggcggcaa
    25321 gcggcggcga gaaccccatt ttgcggcgcg tcaggatttc tcgcggcaca aaaggagcca
    25381 cagcttcctt gaagacgacc tttccccggg tcccccggat cttgaagtgc gacggaaggc
    25441 ggaacgcgag ttcggctaag cggtagtcca gccacggcga tctggcttcg agcgaatggg
    25501 ccattgtcgc ccggtccagc tttaccagaa tatcgccagg caaataggtc tccagatcga
    25561 ccgcttgcat ctgctcgagc ggggataggt ggcgaactgc cgcaaaacgg gcagaaaagc
    25621 tgtcacgcgg cgaatagctc cgaagctccc ggcgcaagcc gctcgacagc acgcagtcca
    25681 gttcccggtc ccgaaaaacc gacatcgagg tgaagtacgc gtggccgatc tcttgagaaa
    25741 ggttcgccag cagggtcttg gcccggaaaa ttcgaggcag gtaatcgaat ttgggataat
    25801 attgcgcgcc caaaccgatc acagacctcc ggaaccagcc gggccatgcg gcgcgcagcc
    25861 ggtcctcgat cacgccaaac aggtagcggc ggtaaccgcc gaaaagctcg tctgcgccgt
    25921 cgcctcccaa ggcgactgtc acgtcctgcc gcgtaacccg agccagatgc agcgtgggaa
    25981 tcgccgatgg atcgccgaaa ggctcgtcga aatggcgagt gaccgcttca attgcgtcgt
    26041 gagcctcggg cgagacaaca tattcatggt gctcggtgtg acagcggccc gccattaacc
    26101 gcgcgaaagc gcgttcatcg aatttcgggc tggtgaaacc gatggaaaag gttttgggac
    26161 gctggcccgt ttcccgagcc agtattcctg cgacggcgct cgaatcaacg cctccactga
    26221 gaaatgcgcc gagcggcgcc tcgctgatga gccggcattt taccgagtcc acggcgagat
    26281 ctcgaagcgc ctcgacggca tcgtcaaacc gcagccgccg gtccgcaaga aaggccggtt
    26341 cccaatacct tcttatgtga tagctggcgc aattcacggt gaggctgctg cccggctcca
    26401 gcttaaatac gttccgataa atcgtctggg ggtcgggaat ataagaaaac gcgagaaaat
    26461 cgcaaacgct gcgaccgctc acgggagcgt caaaaccggg cacggcggtc aaggccttca
    26521 attcggaggc gaagcagaat cggacgcctg gtacggagaa cgagtagtac aaaggcttct
    26581 cgcccagccg gtcccgggcg aggaacattt gctgattttt ttggtcccag atcgcaaatg
    26641 cgaacatgcc gttgagcaac tcgggcattc gctctccggc gtcttcataa aggtggacga
    26701 gcacctccgt gtcgctattg gtggcgaatt tgtgcccctt ctcgatcagt ttgcgccgca
    26761 gggcctggaa attgtagatc tccccgttga agacgacctg gatagatcca tcctcgttgc
    26821 tcagcggctg gttccctccc cgccggtcaa taatgctcag gcggcgatgg ccgagggcgg
    26881 cgtgctggtc gcagaaaaag cccgaggaat ccggtccgcg gtgggacaga cgctcgcaca
    26941 tccgctccac gatgctgcgg tcgggcgcgg ccgaattgca agccaagtag ccggttattc
    27001 cacacatgtc aggtttgccg aggaaggtgt agcttcgccc tttcactcac atcggtgatg
    27061 cgagcgcttg aagacaaaga tccagaagac tacgccggaa ctgccgatcc gagctccgcg
    27121 acgctgcctg gtcgatccag gcgctcagta gtaatagtag tcgtgccgct tccagaacag
    27181 ggattcctga acgcagttga ccaccacgcc gagcaacttg tccggagaaa ccgtctggag
    27241 cgctgtcgaa agctgtgtcc gattcgtatg tccgggccgc acgataagca ctattccatc
    27301 gagcgcggcc tgcaaaagat cgtagtcggc gaccagcccg atcggcggcg catcaaagat
    27361 cacgaattga aaatcctggc ggacccgttg acaaacctcc cgccacgcgg gcgaatcgag
    27421 cagttccgca ggagcgttcc ggttttctcc cgcgggcaag aaatgcaggt tggggaggtt
    27481 ctcaaggcgc acaatcgctg tccccacaga gcagtcgccg gccagcactt ccgccaggcc
    27541 cgggcccgaa cgcactccga gcgcccatgc caaattggcc cggcgaaaat cagcgtccgc
    27601 cagcaacact ttcgcctgct gcttcagcga aattgcagca gctatattgg ccgcgtttac
    27661 ggtcttgccg tcgcccggag cggcgctgct gatccctatc agcttcggct gccgcggatg
    27721 ctgcgtcagc tttgtgcgaa tgattctgta ctgttccgcc gacctccagt gatcgtcgcc
    27781 gaagcgcagc agcggaagtc gcgatttgat ctgaaggctg acggttcgat acggcgagga
    27841 gtggtctccc ctcgccggag cggaatgcaa accatgcaac ccaggcccga gatccgtggg
    27901 cggatccggg agcggggtca ggtttccaaa aatactttcc atggcgtcct tcagacccac
    27961 ccaaagtaaa ttccggcggc tgccatcgct cccagcatga gcagaatcgt cgagaaagcc
    28021 agcacccacc ttgttttcgg ctgtcgcccg cggccatctt cggctctctg acgattcgtg
    28081 cgcggccggt cggcaatgtt ggggtcgatc gtaggcacgc ggcccagaac ccacacgccc
    28141 gcgggcaatt cccattcgcc cagcagaacg tttttcctga cctgcgctcc cacggcaaaa
    28201 gtcaccccca gaaacagccc gagcacggat cccagagcgc tcaacagcag ccggtcgggc
    28261 ttcaacggac gttccggcgc cttcgcggcc tcgagcattg tgaaccgctc cggctgctgc
    28321 cgccgctcca tatcggcggc gacgttggcg gcgagtttct tgtcgagcag cgaacgatac
    28381 gtggcctttg aaatctcgta atcgcgggaa acagccgcca gttgctgttc gcgaatgggc
    28441 agccgttcga tacgcgcctg gtaggcagag atagattgga ctacccggcg gcgctccgct
    28501 tccagcgaat ccaagtcttt ggccgtggct ttcagttgca cttccaggtt ttcaacccgc
    28561 tcgcgctcgc gattgagggt ctgctccatc tgaagctttg ccgaaagatc tgcggcggat
    28621 gaggtctttt cctgcggaga tgcggctgct ggaggagaag cggccgccgc gcgagctgct
    28681 tcctgccgca gctcgcgagc cagctccgcc ttggcgagct tgatatcggg atgatcctcc
    28741 gtatagcgca ttctgagagc gtcgagctcc gcccgaaggc gatcagaccg tttctcagcg
    28801 ggcgccgcgg gcgaagccga attggggccg aggctttccg gagcggcagc agcgctggtc
    28861 tgcgagagag cgtccagaga agtcctggcg ctctcgatcg cattttcgag catcagcttc
    28921 tgctgttccg tgcggctgat tgattcctga agcccctgca actgcatctg gaactggccc
    28981 aaagccgccg ccaaagtgtt ttcctgctgc ggcagctctc cgttgtactt catcttatac
    29041 tcgcttagct tggattcttg ctcctcgagc cgggccttgg cctgcgccag ttgatcctct
    29101 aaaaattgcg aggtcccttc cgcctggctt tcccgcgtcc ggatgttctc gtcaataaac
    29161 aggccgctga tatagttcgc gacttgcgaa acgacggtgg gaacggatcc gatataggaa
    29221 atgcggaaag cgcccgggcg gtccttgcgc caccggctgt cgatgctgat accgatatcg
    29281 ctccgcatgc gctggacgat ctcgtcctgc gaaagggtct tacgcgcctc cggatagaga
    29341 ttgaactttt cgatgatctg ccacaactgg ctggagctga gaatctgctg ggagatcgtg
    29401 ttcaagcgct cctgcaagtc ggcgttcacc gtggaaggaa catatttctc gggaatccgc
    29461 tgcgactcca ccagaaccac cgcttccgct ttgtaaaccg cagggagccg ccggacggcg
    29521 gcaattacgc ccgccgtaac cagggcccag atagcgagga tggagaactt ccgtgtccag
    29581 agcgtgtgca acaacgagac tggtgaaaaa tccgggcgat cagtcacggc ttctcccgtt
    29641 tatttcagca aacacaaaac ggcacttcga tagcaatact actccgctgg gatgctatgg
    29701 caagtcaatc gccaaacaga cggatttttg ttttccggta cggcggaacc catattttgg
    29761 tgctattccc tttagctgat atgccgcccg cgccccttcc ggcgccggcg aacagccgta
    29821 ggacgtgaat tcccaataat tgggaatgga aaaaaactgg gaactgggag ttcttatggc
    29881 ccgccgaggc ccagcagcac agtccgaatg ctttgaaaaa cgatcgagca atcgaacaga
    29941 aatgacatgt tcttcaagta aaacaaatcg tattcgagct tgcgcaagga atcttcaagg
    30001 ctggcggtat agttatgccg gacctgggcc cagccggtga tgccgggacg aaccatgtgc
    30061 cgcagttcat agtaggaaag tttgctcttc agttgctcga cgaagtacgg ccgctccggg
    30121 cgaggcccta cgaagctcat gtcgccgcga atcacattga taaactgcgg caattcatcc
    30181 aggcggtact tgcggatgaa acggcccacg cgcgtgatgc gcggatcatc gcgctgggcc
    30241 cactgggcgc ggccgtccat ctcggcgtcc acccgcatgg atcggaattt caagagctcg
    30301 aagcattttc cgcgcaggcc tacgcgcact tgccggtaga tcaccggacg gccggattgg
    30361 ataagaaccg cgagcgccac cgccagttgg agcgggagag tgacgataag ccccagcaca
    30421 ccgaagcaaa tgtcggtggc ccgcttgatg ccgccgcgaa ggcgggagcg gcggaaaccg
    30481 ccggacatga cgaaaaggct tagccagctt ggccggacag ccaccaacca aaccctcccg
    30541 gtaagcgccg ccagcgcgct atgagcgtct tccacggcta caccgctgac cctgaccttc
    30601 atcagctccg atgtgggcag tgcgccgcgc cagtcctcca tcgccacgat aatccgggaa
    30661 atctggtgcc tcgatgcgat tgcgctcaga tcctcgtaac cgcccaggac cggccgcccg
    30721 agcattggcc gggtaacatt gccgacaaag ccaaccagcc gggcgttcaa atctcttcgc
    30781 gcaagcagct cacgcgcaac tgcttccgcc agttcgcccg accccagtat ggcgacgttc
    30841 tgcgatacaa gcacattggg ccaaaccttg ttgacgctga ttcgaaccag gcagaccgag
    30901 ccgacggtaa gaagaaaagc gcccgccagc acgcggtctc cgagaattct tccaggtatc
    30961 agcggcgagg ccaaacccac tatgaccacg gccgccgcaa tcgattgcag aatatacagc
    31021 aactcgagtc tgcggggccg ccgctgggaa aaatcataga ggttattaca gtagaaggct
    31081 gtttgaacca atatgagcag ccccagcgct tccaaacaga aacgaaccgg aggctcaccc
    31141 tggttccata cgtccagaaa tcggccgcta aacaaaatcg agatgaagat caacaaggcc
    31201 tcgatcatca tcagcgcgag taattttaat gacgtgtact ggttaaatat ctgaatcatt
    31261 cccagggccc gccagattat tcgcaattat ggaaccaatc gtgcagtaga acaaacccgt
    31321 ggggcaatta tgaaaccttg gacggcgagc agcatttctc cgcggattcg agaaaatacc
    31381 ggattgcggt gtgtggtgag gtgtcttatt accctggtgt ttttggctgt tgccggagcc
    31441 caaacccccg ggcatgtggt ccagccagcc ggcaaatccg ccaagatgat agtggaagcg
    31501 cggggcgcgc cgggcgccgc cgcgaaggtg gagaaccggc cggtaaccgt tacgccggag
    31561 ccgtcgaagc tgggtttgcg cgccggatac cggatcggcc ccggcgacgt gctggaaatc
    31621 aacgtctgga aggagcccca gctatccacc ggcgccgctg tggtgcgatc cgacgggagg
    31681 atttcgttgc ctctggtgaa agaggtggac gcactcggcc tcacaccggg cgaactcgag
    31741 cgaattttga gcgagaagtt ttcccagttc ctggaagtcc cggaggtgac tgtcattgtt
    31801 aaggaggtgc acagcgagaa agtttacgtg atcggcggcg ttaagaagcc gggttccgtg
    31861 gatctctatt cgcagctaac cgtgttgcaa gtgctggctg aagcgggtgg tctcactgaa
    31921 tacgccaaaa agggcggcat tttcgttttg aggaagaacg gcgacaagca ggtgcgattg
    31981 cacttcgact acaacgccca gctcaagggc aagcctggga agccggagat tgtgctgcaa
    32041 cccggcgata cggttgtggt cccgcagtga gctttggctg aagcgcaaag ttccgtcgcc
    32101 ccaacacctc tggacggcac gctgccgccg ggccgcttct aggcacaagc gcccatttcg
    32161 ggttgctgtt caggagagac tggcgtccta cgtggctggt catccagtcc caggcgtcct
    32221 ggccgcggag tgggccgtcg ttaattccgg gcagaaagct cgccgctgct tgggcgatgt
    32281 gagtgtaccc gtgctccgca tccgccatgt cggtttcgga gaacccggcc ggcgaagggt
    32341 cgaggaagcc cgctttgact gacccccagt ccggaagaaa gcgccggtcc ggctgccgca
    32401 aaaccggaat ccggtaactc gccacgagaa aaggatcgta gtccggattc aagatctggt
    32461 tcaagaggtt cttagccaca gtttcctgca actttgctac gggaaatcca agctctttca
    32521 ggtgccccag gacgatgtga ttgtaattca acatccaggg cgagctcccg gcgtacacct
    32581 gcttcgtatc caggcccgta gtaaggccgc tgcttccttc ttccagaaag tgcagcgggt
    32641 tgtctgtatt tcctgcgacc gtattccggc cccagcacca ctttgacgtc tctttcaacg
    32701 cgttataggg agaagtgtgg caaggctcat agaaggagcc atcggtgata ttgtatttgc
    32761 cttcccggat ggcgatattg ctatacagtt tttccgtaaa gtaggctttt tccagcgcgc
    32821 catcgggggc ggcaaaggcc gcgtgggcca gattgcgcat tccccaggcg acgccgcgga
    32881 tttcttcgct gtcattgatg aaaccccagt cggcgtgccg gtcgtaagag agggagactt
    32941 gcggatttcc gtgagccaca tcaaaagaag cccaaaaata tatttcctcc aggaagtacc
    33001 aatcgcccgt gatcagatag ggaatgtaag caaagctcgg ctggtgcgcg aggtccacga
    33061 tccatttgcc gttgcttgtg gggccgatgg gaaccaccaa atcttccgct cgggtatctt
    33121 gtgtctgcgc cgtacttcgg aatgtgggcc gggcgtcaat ggaaagcacg cggccccccg
    33181 cgtcttccgg ctgtgacttc agaaaccgcc gccccaggtc cgactcccgg tagtggatcg
    33241 gcacgtagcc gctgacagca gcatccccca aaacaacctc atacaggtct gggtcgaacg
    33301 tatatagata tcggacatac catcgcggga acaggccgat atccccgcgc ccgcctgttg
    33361 tagggaaggc ctgcatccat tggccgtggc cgccgatgtt tccctggtcc ccggagcgga
    33421 atgatacggt ctcgctcagg acatccgcgg gccccacgac cttggtcaga tcgtaggacg
    33481 ggatcgcctg ggagtgaatc aggtacggaa gattgtagtc gattcgaacc gggtagggct
    33541 gcgggccgct ccaaaatacc tttcgccacc gcgtcttggc gatgtgattc acggcgggat
    33601 tggagtatgc cgcagcggct gccggaggat tgcccagacg aatctccagc gaataagtct
    33661 ggtccttcag tttggtggac caggcgtttt ccaagatggc ttcgatcctg accgaaggga
    33721 atcccctgta gacgctcacg acaaaaatag gatgaagtga tttaccgccg gcatccgccg
    33781 aatcggacca tccgccgctg caaggagaat cgccgcttgt acagtcccat ccgaagtcgt
    33841 aagcaactgt cgccggattt ttatcctcca cgatcaactg ggagacgacg gggcctttca
    33901 gccagtagcg gacgccgaga tcgtcttcgc gcccatccca gttggccagc atggtccgga
    33961 ggtccgccgt gtgagaggag ctttgagtgg ccgtaaccac tgcacccaga taggacgccc
    34021 agggagcggc cggatcgaag aggtcatctt tcgcgagaaa cggcttattg tttccatcgg
    34081 actgattaac gaacgccacg gtggcggttt tcctttcgcc gatttccgtc acaaacgaca
    34141 gcattgcgtg ctgcaagctt ccatccggcc agcgggtctt gacgtcgcat tgggtgacta
    34201 ccagcgcgcc gtccacctct gcctgtgcaa aatcaggaat ctctccctgg gcgaagatgc
    34261 gcgaaatagt gaacggctgc ctggttttcg gcagaccact gaggtcggtg atcgtgatcg
    34321 agttggaggg cgcggcgccc agggttcctg cggctgtcgc gaacaggacc aagaggcttc
    34381 ggacgtgcgt tccgaagacg cttgcgttct tcatttcctg cggctgcaat tgccggaaga
    34441 agcgttttac acgcagaacg caaactaaac caatggccgg cccgtaccgg aactaaatgc
    34501 tcccaaaaaa cgtcgaggcc catgacgagc agcatggtaa gcgggactgg gcagcgtctc
    34561 ctgagatctc cgctcgtcaa ccgcagaagt ttctgtgcgc tcgcgatatt tgaggagagg
    34621 ccatgactcc aggctgagat caagcaaatt tcccaggcgg atctgtcgcc tatattgtcc
    34681 aattttttcg aagttgccag gccttgggat gtatgttctt tcactgaatt tcaggagggc
    34741 tcttctcagc aagcctcaag gtcgccagct tccgggacga ggcgctgcgg gttgattgtc
    34801 gatacgccca agaagccggc gttctcctga atcggcgcgg cgtccgagga ggatgctata
    34861 ctcctccggt gtgaagcggc ggctctggcg tggcagcggg cctgctgttt cgccggtctg
    34921 ggaaggcgcc cgctcccttc cagcttgcgc gaggcttgat caggcgtttc ggacagcggg
    34981 cccgaaagtc acggattcgc tgctgttttt tttctgcgtt tcgttcggat cccggatgcc
    35041 gtacgaggat cgatgaggtt ccttcgccgt tgttggtggg ccgcgtgctg tatcggcatc
    35101 ctggccgggc aaggggttgt gaacacgcag cgcgcgccac gttcctcccc gaaggcccgt
    35161 accaatcttc cgattcccag agtgaatttt gaagatgttg ccgcggcggc gggcctgaat
    35221 ttccgcaacg tggcagggga ccggaccgag aagctataca tactggaagc caccggcggc
    35281 ggggtggcta tcttcgatta cgataacgac ggctggcccg acattttcct ggtcaatgga
    35341 tccaagttca actttgcagc ggacgaggag cgccccgtca gccgcttgtt ccgcaatctc
    35401 ggcgcacacg ccgaccctcc cggcctccgc tttgaggacg tcactgaaaa agcgggcctt
    35461 tcgcgatccg gctgggggca gggcgcctgc gtgggagact acgacaacga tggctttgaa
    35521 gatctcttcg tcacctacta cggcgccaac gttctctacc acaacaacgg tgatggcacg
    35581 tttcgggatg tgaccgcggc ggccgggctt cccacttctg gcacccgctg gtccacgggc
    35641 tgctcgtttt tcgactatga tcgcgacggc cggctggaca tcgccgtggc caactatttg
    35701 caatttgatc cctcgcaggt tcccaaaccg ggcgagactc cattctgcgt ttacaagggc
    35761 ctcccggtga tgtgcggacc gcgagggttg ccggcgggcg tcaacattct ttatcacaac
    35821 gagggcggcg ggaaatttcg cgacgtctct gagcagagcg gcttcagcaa gccggagggc
    35881 cgctacagtt tcgccgtgct tgcggccgat ttcgacaacg acggctggcc ggacgtctat
    35941 atagcctgcg attcgacgcc gagcatcctg ttgcacaaca atcacgacgg aacctttacc
    36001 gacatcggcg tcctttccgg cgcggccttc aatgaagacg gccaggagca ggctggcatg
    36061 ggcgcagcgg ccggcgatta caacaacgac ggatggctcg atatcgtaaa gaccaacttc
    36121 agcgacgaca ccccgacgct ctacctgaat aataaagacg gcacttttac ggacgttacc
    36181 tacgctgccg gtttgggggt gaacaatcgt tttctgggtt ggggagttgg cttcgtggat
    36241 attgataacg atggctggaa ggacatcatt atggtcaatg gccatgtata tccgagcatc
    36301 gacagcttga agctgaccag cccataccgt gaggaaaaga acgtctattg gaacctgcgg
    36361 aacggcgcgt ttctggatat ctcggcgcgg gcgggacccg cgatcctcga ccgccgcgct
    36421 gcccgcggcg ccgccttcgg cgacctcaac aatgacggcg cgctcgaagt agtcatcaac
    36481 aatattgatg atgcgccgag cctgctcgta aaccggggag agaagaagaa ctggcttctg
    36541 gtaaggacgc agggaaccaa gtcgaaccgg gacggcatcg gagcccgtgt gacagtaacc
    36601 gctggttccc tgcaaatgag cgcggaggtg ggcagcggct caagctttct ctcgcacaat
    36661 gatctgcgcc tgcactacgg cctggggaat tccaagcagt ctgacaacat ccgcgtccgg
    36721 tggcccagcg gcttggagga ggtcttcccc ggcagccccg ccaacaaaga ggttctgctc
    36781 gtcgaaggca agggccaccc cgcagggaag taggcgggag agggctggtg agtcagctta
    36841 gcacggattg gaggcgtgac cgcggccaac tgtgggcgag cggctgaaag ctctacttag
    36901 gctcccgaag ctttggcagc cagagcgact gtcgagctgc gcagacttct gctcaactga
    36961 agagcgacgg ctcgtctttt ttcgtttttt ccctgtgagc gccgggcggc ggcgtggcgc
    37021 ggcgccacgg gcatagataa accgtcgctt caccaggccg tatcattggc atctctcact
    37081 cgtggtgctc gtgatcgcaa gctgcatttg gaatcgtcag gacccgtgcc gatcggtggc
    37141 ctccgggcaa gcccgaatgt ttcgccgcca cgccgaggta accgggcggc cgcaatcgaa
    37201 aattctatgg cggcgcccgc ggtctgcgcc tgggcgttcg gttcccatgt aatataagag
    37261 ttgttcgggc gccgtatcgt gtaaggcgct cccagcctgg attcatacgc ttgtcttatc
    37321 cgatggaagc agggaggtac acgtgagcaa gacagccggc cgacttattc aaatactggt
    37381 tgtggctatt ttgggagggt tcctcgtgcg aatgcacacg caggcgcagg aggctgctcc
    37441 cgagcaatac gcgttgccgc tgccgataga ccgaggcgcg gcgtcggtat ggcaaagcct
    37501 caagaaactg cacacgcgcg ccagcctgat gatggtgacg gcgcacccgg atgacgagga
    37561 cggcgcggtg ctggcctacg aatcgcgcgg cgtcggcacg cgcgtcgtgc tgctcacgct
    37621 gacccgggga gaaaccggag ccaacgtgat gtctcccgat tacttcgacg ccctcggcct
    37681 ggtccggacg caagaactgc tggcggcgga ccgttactat ggggcgcagc aatattttac
    37741 ccgcgtcatc gactacggct tttcgaagac caaggaggag gcgctggagc agtggtccca
    37801 cgaccgcgtc ctggccgacg tcgtgcgcat cgtccgcatg acgcgcccgc tggtgattac
    37861 ctcggtattc gtaggcggca tcacggatgg gcatggcaac caccaggtgg cgggccagat
    37921 ggctcaggaa gtcttcctcg ccgccggcga ccccagcaaa tttcccgacc agattcaggc
    37981 cggccttcgg ccctggaagc ctctgaaggt gtacgcccga gtcccgttcg tgggccgcat
    38041 gtcagaaaaa gggctttatg attacgccac tcatacctgg catccggcgc gcctctttga
    38101 ctatgtccgg caacagtggc tgccggtgcc gctgccggcc aatgtagaag ttccggtcgg
    38161 cgattacgat cccgtgctcg ggctgaacta cagccagatt tcccgcgagg gtctcggcca
    38221 ccagaaatcg cagaacggcg gcaccggcat gccgccggcc ggcgcggcaa cgcgggcgta
    38281 ccaccggttc ggctcgatcg tgccggcaag cgagaaggaa acatcctttt tcgacggcat
    38341 cgatacatca ctgacgggta tcgccggcct tgccccgagc ggcgacacgg cctttttgaa
    38401 gcgcgggctc ggcgagatca accgaatcgt cgagcaggcc atggcggagt tcaacgccgc
    38461 gcatccggag agcatcgccc cgcgcctggc cgccgggctg aagacgaccg ccgcattgat
    38521 cgatcaggtc aaggccagca gcctgccggc cgacgcgaag caggatatcg tctacgagct
    38581 ggaagtaaag cggacccagt tcaacgaggc aatcgcgcag gctttgggaa tctctctgtt
    38641 ggctacggtc gccccggaaa aggaaccggc cggcatggct gccatgttcg gcatgacgcc
    38701 ggaaagcttc caggtggcga ttccgggcca gcagttctac gtgaaggtgc acgtagtgaa
    38761 ccagagcaag aatccggtcg agctgaagga tgtctcgctg agggggccgg aaggcgagga
    38821 ttgggccatc gagcggcagg gagcggccgg cggcgcgctg gccaacaatc agccctcgga
    38881 cattcgcttc cgggttcatg tgccagggaa cgcgcacttc acgcggccgt atttcacccg
    38941 tcccaatatc gaacagccgt attacgacat acaggacccg aagtatctgg gccagcctct
    39001 cccgccgtat ccgctcgccg ctcgcgtgga actgaactat gaaggccagc ccatcgaggt
    39061 gtcgcaggtc gtgcagactg taaagcgggt cactggcgct ggcgacgtcc tgcaaccgct
    39121 ggtggtgggt cctgcggttt ctgtctctat ttcgccgagc gccggcgtcg taccgttgga
    39181 cgccaagtcg ttccaggtct cggcgaccgt ccacagcaac gtgaaagggc cggcgcaggg
    39241 ggttgtgcgg ctcgatctgc cggccggctg gcgctgctcg cctgagcgcg cgccgttcgc
    39301 caccagcaag gacggagagg atcaatccgt gagcttcgag gtgactccgg cgaacctgcg
    39361 gccgcagcct tacaaaatcg ccgccctcgc tatactggca gggaacgaat accgcgaggg
    39421 ctaccatatg gcgggctacc agggcttgct tccctataac ctgtaccatc ccgccgtcta
    39481 caagacgacc ggcgtggacg tcaagatagc caagggactg aatgtgggtt atgtcgaagg
    39541 gagcggcgat gaagttcccc agtcgctcga gaaccttggc gttcaggtcc attacctgac
    39601 gccgctcgaa atcgcgaccg gcgacttgca taaatacgac gttatcctgt taggcgttcg
    39661 cacgtacgcc gtccgcgaag acctgaggac ctacaacggc cgcttgctcg attacgtgaa
    39721 gaacggcggc gtcgttgtgg tgcagtacaa cacgccggaa ttcgaccata actacggccc
    39781 atatccctac acgatgacaa acgatccgga ggaggtgacc gacgagcaat cgaaggtcga
    39841 gattctcgac ccgagcaatc ccgtattcac ctggccgaac ctcatcacgg aggacgactt
    39901 caagggttgg gtggaggaac gcggatcgaa attcatgagt tcgtgggacc cacattacga
    39961 ggcgctgctt tccaccaacg atcccggcca ggcgccgcag aaagggggac tgctgtatgc
    40021 gcgctacggc cagggcgtat acatttacaa tgcctatgcc ttctaccgac agttgcccga
    40081 aggcgtgccc ggcgcctacc gcatctttgc caacttggtg agcctgccgc gccatccgca
    40141 gttgcgcgcc gcaagcgccg cgccgtcccg aacggccggg cgctgaggtg caaaatcccg
    40201 gcgagcgaaa aggggcgccg gctggccagc cgggcctgat ccggggcatg ggtttgccgc
    40261 aagccacggc ggcaaacatg ctgaacatga tcggcatcgg gcccttcatc accattcccc
    40321 tcatcctttc cacgatgggc gggccgcagg ccatgctggg ctgggtcctg ggcctgttgc
    40381 tggcaatctg cgacgggctg gtttgggccg agttgggggc ggccatgccg ggcgcggggg
    40441 gcagctacat ctacctgcgc gaagcgttcg ggccgcgcac ctggggcggg ctgatgtcgt
    40501 ttctgttcat ttggatgatc gttttcatcg cgccgctttc cgcggcttcg gccgccgtcg
    40561 gcttcagcaa ttatctgaag tatttgtggc cgggcatgac gcacacgcag gcggttctgg
    40621 tggcggtagc ggtctgcgga gccgtcaccg ccctgcttta ccgcaacatc gaatctgtgg
    40681 gctggctttc ggtcgtgatg ctgtgggtcg tggtcctcac tacgctttgg atcatcggcg
    40741 cgggcctcac gcacttcaac gccgcgcgcg tgttttcgtt ccccccgggg gctttccggc
    40801 tggatcgcgc tttttttcag tcgctgggag cggccacgct gatcg
//
LOCUS       DASREK010000009        15203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_3957, whole genome shotgun sequence.
ACCESSION   DASREK010000009 DASREK010000000
VERSION     DASREK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 15203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15203
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3957"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFA54_00630"
     CDS             complement(<1..573)
                     /locus_tag="VFA54_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZS49335.1"
                     /translation="MRPGGSKPRALALFTAGVYAFLHLPVLILIAFSFNESRFTVWTG
                     TSWKWYCELAHSQRILEALQNSLLVAAAATALSTVMGTLCAIAMSRRRRPSDRALDAV
                     MQLSIVTPEIVTGISLLAFFQWIFRFLDLGLGLGTIIIAHVAFSIAFVLLVVRARLAA
                     LDPALEEAAMDLGAGPAATFFRVTLPLLQPG"
     gene            complement(570..1448)
                     /locus_tag="VFA54_00635"
     CDS             complement(570..1448)
                     /locus_tag="VFA54_00635"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS49336.1"
                     /translation="MKIREAGQKPPFSNSSVSRSSYWLLLAPAWVWMTAFFLLPLVIV
                     LVYSFLSKGDYGGVALPVTCSNYGRFFDPLYLRILARSFLIAAAATALCLAIGYPLAY
                     CIARYGGQWKNALLFLVVLPFWTSFLVRTYAWIFLLRDTGLINTLLIRLGWVRVPLPL
                     LYNEGAVLVGLVHGFLPLMVLPLYASLERLNPALLEAAADLGARPFTSFFRVTLPLTR
                     SGVYAGSVLVFVSALGAYITPDLLGGGKSIMIGNLVQNQFTTARDWPFGSAASLLLMA
                     VVAVALQLYRTEGDFR"
     gene            complement(1445..2410)
                     /locus_tag="VFA54_00640"
     CDS             complement(1445..2410)
                     /locus_tag="VFA54_00640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HZS49337.1"
                     /translation="MDLAGKRICLTGGGGFLGSFLAEALQERGCRQIFIPRRREYDLT
                     TVQGIERMFETARPQVVFHLAAVVGGIGANRANPGRFFYENAVMGIQLLEYARRYNVE
                     KTIVVGTVCSYPKFTPLPFREDDLWNGYPEETNAPYGIAKKALLVQCQAYRQQYGMNA
                     IYLLPVNLYGPRDNFDLESSHVIPALIRKFLEAKQAGAEEVACWGDGSPTREFLFVRD
                     AAEGLLLAAERYDRPEPVNLGSGCEISIRELSEMVARECGYQGKIIWDTSKPNGQPRR
                     CLDTSRARNSFGFQARTGLAEGLQATIHWYRSAAKAATEPGERKT"
     gene            complement(2499..3515)
                     /gene="gmd"
                     /locus_tag="VFA54_00645"
     CDS             complement(2499..3515)
                     /gene="gmd"
                     /locus_tag="VFA54_00645"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZS49338.1"
                     /translation="MKTALITGVTGQDGSYLAEFLLHKGYVVHGIKRRSSSFNTERVE
                     GIYRDFHEPETRFFLHFADLGDSSSLSRLLHDLRPDEIYNLGAQSHVRVSFDIPEYTA
                     DITGCGTVRLLEAIRQTGLKTRFYQASSSEIFGSSPPPQNESTPLHPRSPYGCAKAFS
                     HYITVNYRESYGLFACNGILFNHESPRRGETFVSRKISRAVAHIKFGLQEKLYLGNLD
                     ARRDWGYAPEYVQAMWLMLQQEEPDDYVIGTGVSHSVREFVELAFDCAGLDWRRYVEI
                     DPRYRRPAEVDDLLADPGKARRKLGWRHSVSFEDLVRLMVDADIAALAARKAGVLAPA
                     SLAP"
     gene            complement(3591..4676)
                     /gene="tsaD"
                     /locus_tag="VFA54_00650"
     CDS             complement(3591..4676)
                     /gene="tsaD"
                     /locus_tag="VFA54_00650"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZS49339.1"
                     /translation="MFLLGIESSCDETAAAVVESGERLLSNIIASQFDTHRRYGGVVP
                     ELASREHLRAIVPVVRQAVEDAGLSYRDLGAIAVTRGPGLVGSLLVGLVYGKALSLSF
                     DIPLIGVNHIEGHIHAVLLELREKQQEIEFPALALVVSGGHTHMYYVRRDGSEYDYRL
                     IGRTRDDAAGEAFDKVAKLLGLGYPGGPVIDRLAPYGDPEAIHFTRAKMKGNDLDFSF
                     SGLKTAVLRHVQAAGMAEEIQRRRALLRENPRPSVEQWLEITPKSTLDLIASFQQTVT
                     EELLRHALRAAEQRDVRSLIVSGGVACNRRLRERFSTLAGPYPVYFPSPKLSTDNAAM
                     IAAAAYPKFLKGEFEDLSLAADASLSL"
     gene            5245..5994
                     /locus_tag="VFA54_00655"
     CDS             5245..5994
                     /locus_tag="VFA54_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS49340.1"
                     /translation="MVPAILTRGLRKLYGNKAAVDGLDLEVPQGSFFGFLGPNGAGKT
                     TTIHILIGLIPPTSGEVQVLGLPLASHLQEIKRRIGVVPDENVLFDRLTGFEFLQFAG
                     RMYGLERNVARARARELLELFELDQEPRKLIAEYSKGMKKRIALAGALIHRPQLFLLD
                     EPFEGVDPMGARLMKEILLGQVRQGATIFLTSHVLEVVERLCDRVAIINEGRIVIEGS
                     MRELRSAEDTLEDTFVRVVGGERAAQSLDWL"
     gene            6002..7738
                     /locus_tag="VFA54_00660"
     CDS             6002..7738
                     /locus_tag="VFA54_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49341.1"
                     /translation="MRLSALVHWPFRVLMARSPAGQSGWKLPMMAAEATRQIQAVLHI
                     QWLSFRNGLRRRSERIGLLFSIFVTTIWYAIWLAAAAGLCLFSLWASTGRLSRTLPAL
                     LLLLLVYWQASPLLTASMGVSIDLRKMALYPLDVKTLFLMECLLRLMTSAEMILVLSG
                     LTVGMAMRAPQQIPIFLPAAALFVLFSVLLSAGLRNLLERLLQRRGFREMFLFLIVMA
                     SATPQFILWTGSGEKVGRRVYRIFHSLPQFMLPSTFLAEAYLGRSTFSNWILMIGWCT
                     AAASFGFWQFRRSFYPDFGSGGANHAPPFSRSDDRERPLNRWPGLVARLLPDPWAALV
                     EKEVCYLSRSPRFRFLFLMGCSFGVVAWLPFGMSRGPLATGVLQASFLTVLSLYALLL
                     LGQVTFFNSFGFDRAATRYFFWSPVSPAAVLAAKNLAAVAFLVLEIFALSSICLLLRM
                     LNGFWQFAEALAVTLISSFYLLSVGNLTSILFPAALSPERVSRAGSGRGVQGLIVFLY
                     PLLIFPVLAAYFARYYWHSRNGFLLLLAAAAAGGLALYLATLPLAARLAFLRREKLLQ
                     DLSRGEGPLVSE"
     gene            complement(7728..8435)
                     /locus_tag="VFA54_00665"
     CDS             complement(7728..8435)
                     /locus_tag="VFA54_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS49342.1"
                     /translation="MEPLIRVENLSHRYDDGTQALRGVDFVLYPGETVAVFGPNGSGK
                     TTFLLCLSGLLESEGRIEICGLPAERKNFPAIRQKAGILFQDSDDQLFMPTVYDDVAF
                     GPLNLGLEAAEVDRRVRECLRLVDMEAAAGKAPYHLSAGEKRRAALAGVLAMQPEILF
                     LDEPVTFLDPPGRAALVDILGRLPQAKLIISHDVDLARELATRAIFFQNGQIAASGPV
                     EEIIERFRWDTTPRFTH"
     gene            complement(8423..9145)
                     /locus_tag="VFA54_00670"
     CDS             complement(8423..9145)
                     /locus_tag="VFA54_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZS49343.1"
                     /translation="MHHVTLDSWSRIGSPIHRLDARVKILAVLAVLLSLVLTEHAYLW
                     LFASYFSLIFTLAWLARLPLAALLLRAALVLPFAATVAALNVFGGTAVRAAAVLGKSY
                     LSALAVLVLLASTPLHVLLRGLESLGAPRFFLMVAQFVYRYLFVLSEQAQHMKQARRC
                     RAGQGGRGGRSLGASAAGALAVLFARSYCRAERIHHAMLARGFQGQFAAARPPSLRAA
                     DLVFLSVTGAVIAALQVLLWNR"
     gene            complement(9123..9428)
                     /locus_tag="VFA54_00675"
     CDS             complement(9123..9428)
                     /locus_tag="VFA54_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HZS49344.1"
                     /translation="MRRLVLAFGFATLGLVAAGVLISSTFPDGLERVAATLGFASRTR
                     NAGLASPFANYEARFFHSRWPAQASAGLLGAALLYGFGVLFGRTLKRKRRNAPRNTR"
     gene            complement(9432..10067)
                     /locus_tag="VFA54_00680"
     CDS             complement(9432..10067)
                     /locus_tag="VFA54_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HZS49345.1"
                     /translation="MHIPDGFLSTPVWVSLDAAAAPVIGYFVRRTQARFDETRVPLLG
                     VMGAFVFAAQMINFPVGAGTSGHLVGGALLALTLGPWAATVVMTAILVVQALVFQDGG
                     ITALGANILNMALAGVWLGHWAVKLIGGGAARQRAAVFLGGWLSVFGAALLATVELSL
                     SHVAPNRPLFASMLGIHAVTGFIEGVITVAVLEAIARLSPRFIFRGEQMES"
     gene            10308..11381
                     /locus_tag="VFA54_00685"
     CDS             10308..11381
                     /locus_tag="VFA54_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS49346.1"
                     /translation="MEPIVELREVVKSFGARVAVNHVSLAIPRGRFYSLLGPSGCGKT
                     TTLRLIAGFDRPDSGDVLIHGVRVNDLPPYRRKVSTVFQSYALFPHLTVAGNVAFGLK
                     QAGLPKDKIRARVQEALELVQLSKFAHSLPRFLSGGEAQRVALARSIVLEPDVLLLDE
                     PLAALDQRLRREMRVELKRLQRAVGITFLFVTHDQEEALTLSDGIAVMNSGKLEQVGT
                     PEEIYRSPRSRFVASFVGSSNLIEGRVTRVEQGRLILTTPAGRCFSVPCPPSPPPVGS
                     LAGLLVRSEAIQIANGSAPSQAQRLTGKVVQSMFLGPYRQIVVQVDPEIELTVLCPAA
                     TPLPADASLELWWNPEDCLILPG"
     gene            complement(11472..13220)
                     /locus_tag="VFA54_00690"
     CDS             complement(11472..13220)
                     /locus_tag="VFA54_00690"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZS49347.1"
                     /translation="MNAGEFGSRTIAGPLALYTSRWTRLARSLWLLCGFFGFFLAESK
                     AQTYDIIIRNGHVIDGSGSPWYAADVGIRDGRIASIGNLAEAQAKRVIDAQRMVVAPG
                     FIDMLGQSELTVLVDPRLPSKIYQGITTEITGEGNSVAPLNDAIVRADRLSFEHLKIV
                     PDWRNFQQYFARLERQGIGINLASYVGATTVRRMVIGEDDRPPSPAELERMKSLVRDA
                     MQQGAIGLSTSLQYAPAPYATTEELIALAAEAAKFGGVYATHMRSEGNAVIAALDEAI
                     RIGREAKIPVQIWHLKAAGKSNWGRMPEIIEHIEKARRSGVEIGANTYAYTAWFNSFS
                     AFIPPWAHDGGDAKLIARLKDPAARARIRKDLETPSDAWDNEWQAIPGPEAIQVCVVQ
                     NPKLLPLQGKTIAEIAGQWGKDPIETIFDLLVEDNAYTYVAVFGMSEPDVTLALQQPW
                     VSIDNDSQGTSPEGILGKKHPHPRAYGTFPRILRKYVREEKKLTLEDAIRKFSALPAG
                     QMRLADRGLLKSGLWADIVVFDPDKIRDLATFENPNRLSEGMQYVLVNGTPVIDGGKM
                     TGARPGKVLRGPGYRP"
     gene            complement(13217..14779)
                     /gene="pruA"
                     /locus_tag="VFA54_00695"
     CDS             complement(13217..14779)
                     /gene="pruA"
                     /locus_tag="VFA54_00695"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HZS49348.1"
                     /translation="MQLSEFQNEPLTDFSATENRAAMETALEKVRAQLGREYPLLIGG
                     QRVSTENKIRSINPAHPEQIVGVHAAADVELARRSIAAANDYFRCWSAQPVEQRARVL
                     LETARIFRRRRHEFSAWMIYEVGKPWAEADADVAEAIDFCEFYAREALRLSGPQPLTP
                     VPGERNELRYLPLGAGVVIPPWNFPLAIATGMTTAALVSGNTVVLKPSSDSPTIAAHL
                     VEALLEAGCPPEAISLVTGSGSSIGDALVEHPLTRFVAFTGSKQVGLHINELAAKTQP
                     GQRWIKRVVAEMGGKDAIVVDADCDLDKAVAGVLVSAFGYQGQKCSACSRAIVVDAVY
                     DKFLERLVERTRQITVGPPEDPRNYMGPVINERALRSILDYIETGRREGQLLTGGRRA
                     PGHGYFLEPTLIADVEPRARIFREEIFGPVLAVTRARDFDQALEMANDSEYGLTGAVY
                     TRDREKLKKAAESFYVGNLYLNRKCTGALVGGHPFGGFNMSGTDSKAGGRDYLLLFMQ
                     AKVISEKIEDGG"
     gene            complement(14892..>15203)
                     /locus_tag="VFA54_00700"
     CDS             complement(14892..>15203)
                     /locus_tag="VFA54_00700"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZS49349.1"
                     /translation="LRAGAKVYNPYRRKHMARGDGGEKVMWFLAGVAIGAAVGLLFAP
                     KTGRELRQLLGEKTAEGKDRLVDAGREIYDRGREIFEKGKDLADDAAELFERGRKAVR
                     L"
BASE COUNT         2923 a         4825 c         4532 g         2923 t
ORIGIN      
        1 gcccggctgc aagagcggca acgtgacccg aaaaaacgtc gccgccgggc cggcgccgag
       61 atccattgcc gcttcctcca acgcagggtc gagcgccgcc agccgggccc gcaccacaag
      121 cagaacgaaa gcgatggaga acgccacgtg tgcaatgatg atcgtgccta ggcccagccc
      181 cagatcgagg aagcggaaga tccattgaaa gaaagcgagc aacgaaattc cggtgacaat
      241 ttccggcgtc acgatggaga gctgcatgac cgcatcaaga gcgcggtcgc tcggccggcg
      301 cctgcgggac atggcaatag cgcacagggt tcccattacg gtagaaaggg cggtcgcagc
      361 cgcagccacc agcaggctgt tctgcaacgc ctcgagaatc cgctgactgt gagccagttc
      421 gcagtaccac ttccaggaag ttcccgtcca cacggtgaaa cgcgactcgt tgaagctgaa
      481 agcaatgaga atcagcaccg gcagatggag aaacgcataa actccggcgg tgaacaaagc
      541 cagggcacgc ggtttcgagc ccccgggtct catcggaaat ccccttccgt gcggtaaagc
      601 tgcaaagcca cggctaccac cgccattagc agaagcgaag ccgccgagcc gaagggccag
      661 tcgcgggccg tggtgaactg gttttgcacc agattgccga tcatgatact cttgccgccg
      721 cccaacaggt cgggcgtgat gtatgccccc agcgcggaaa cgaatacaag caccgagcct
      781 gcgtaaacgc cggagcgagt cagcggcaga gtcacccgga aaaagctcgt gaaagggcga
      841 gctcccaaat ccgccgccgc ctctaaaagc gccggattca ggcgctcgag cgaggcgtaa
      901 agcggaagca ccatcaaggg cagaaaccca tgcaccagcc ccaccagcac cgcgccttca
      961 ttgtaaagca acggcaaggg tacgcgaacc catcccagcc ggattaagag tgtatttatc
     1021 agacctgtat cccgcagcaa gaagatccac gcgtaggtgc gcaccagaaa gctggtccag
     1081 aaggggagca ccacaagaaa cagcagcgcg tttttccact gccccccgta gcgggcgatg
     1141 cagtaagcca gcgggtagcc gatcgccaga cacagcgccg tggccgccgc ggcgatcagg
     1201 aaactccgcg cgaggatgcg caaatacagg gggtcgaaaa agcggccata gttgctgcat
     1261 gtcacaggga gcgcaacacc gccgtaatcc cccttgctga ggaagctgta gacaagtacg
     1321 atgacgagcg gcagaagaaa aaacgccgtc atccacaccc aggcgggcgc gagcaggagc
     1381 cagtacgagg atctggagac cgacgaattg gaaaaggggg gcttttgccc ggcttcccga
     1441 attttcaagt ctttctctct ccaggctcgg tggccgcctt agccgcgctc cgataccaat
     1501 ggatcgtcgc ctggagccct tccgccagcc ctgtgcgggc ctgaaagcca aaggaattcc
     1561 gcgcccgcga ggtatccagg cagcgccgcg gctggccatt tggcttggag gtatcccaga
     1621 tgatttttcc ctgatagccg cactcgcgcg ccaccatctc cgagagctcc cggattgaaa
     1681 tctcgcaacc gctgcccagg tttaccggct caggccgatc gtagcgctcc gccgccagca
     1741 gcaacccttc cgcggcgtcc cgcacaaaca aaaactcgcg cgtgggtgag ccgtcgcccc
     1801 agcaagccac ctcttcggcg ccggcctgct tcgcttccag aaacttcctg atcaaagccg
     1861 gaatcacatg ggatgattcc aaatcgaaat tatcgcgcgg cccgtagagg ttgacgggaa
     1921 gcagataaat cgcattcatt ccgtattgct ggcggtaggc ctggcactgc acaagcagcg
     1981 cctttttggc gattccgtac ggggcattgg tctcctccgg gtacccgttc cacaggtcat
     2041 cttcgcggaa cgggagaggc gtgaatttag ggtaggagca taccgtcccc accacgatcg
     2101 ttttttcgac attgtatctc cgcgcgtact cgaggagctg aatgcccatc accgcgtttt
     2161 cataaaagaa acggcccgga ttcgcgcgat tcgcgccaat gccgccgacg accgctgcca
     2221 ggtgaaacac cacttgcggg cgtgctgttt cgaacattcg ctcgatgccc tgtacggtag
     2281 tcaggtcata ctcgcggcgt ctgggaataa agatttgacg gcaccctctt tcctgcaaag
     2341 cttcggctag gaaagagccg agaaatccgc cgcctccggt cagacaaatt cgtttcccgg
     2401 caagatccat gagaaatttc gggcttttac tgaatcatac accggccgcc agggccttgc
     2461 cgcggcatct cgatttcgag aggtatcacg ccgggcgctc acggtgcgag cgatgcgggc
     2521 gccagcacgc cggctttacg cgcggccagc gcggcgatat cagcgtccac cattaatcgg
     2581 accaagtctt cgaagctgac gctgtggcgc catccgagct tgcgccgggc cttgccgggg
     2641 tcagccaaca ggtcgtcgac ctcggccggg cgccggtagc ggggatcgat ctcgacgtac
     2701 cgccgccaat cgaggccggc gcagtcaaaa gccagttcaa cgaattcccg cacggagtga
     2761 gagacgcccg tcccgatgac gtagtcgtcc ggctcctcct gctgcaacat gagccacatg
     2821 gcctggacgt attcgggcgc gtagccccaa tctcgccggg cgtccagatt gcccagatac
     2881 agtttctctt gcaatccaaa cttgatatga gcaacggcgc ggctgatttt ccggctcaca
     2941 aaagtctcgc cccgccgcgg cgattcatga ttgaagagaa tgccgttgca ggcgaacagg
     3001 ccgtagcttt cccggtagtt aacggtaatg tagtgcgaaa aagctttggc gcagccatag
     3061 gggctgcgcg gatgcagcgg ggtgctttcg ttctgcggag gcgggctcga gccgaaaatc
     3121 tcgctggaag aagcctggta gaaacgggtc ttcagacctg tctgccgaat cgcctcgagc
     3181 agccgaacgg tcccgcagcc ggtgatatcg gccgtatact ccggaatatc gaaactcacg
     3241 cgcacatggc tctgcgcgcc caggttgtaa atctcgtcgg gccgcaggtc gtggagcaga
     3301 cgcgagaggc tgctggaatc gcccaaatcg gcgaagtgca ggaaaaacct ggtctccggt
     3361 tcatgaaagt cgcgatagat gccctccacg cgctctgtgt tgaagctgct cgaccggcgc
     3421 ttgatcccgt gcacgacata gcccttgtgg agcaaaaact ccgcgaggta agaaccatcc
     3481 tgcccggtaa cgccggtaat gagagcggtc ttcatgagcc ctgctttcca gcgaaatcag
     3541 cagcgcggcc gttgcgtgtt cccactgctt tgccgctgaa agcctgtcta ttataggctg
     3601 agtgatgcgt cggcagccag cgagagatct tcaaactcgc ctttcaagaa cttgggatag
     3661 gcggcggcgg cgatcatagc ggcgttgtct gtggaaagtt tggggctcgg gaaatagacc
     3721 ggatacggac cggcgagcgt tgagaagcgc tcccgcagcc tgcggttgca ggcgactccg
     3781 ccggagacaa tcaacgagcg cacatctcgc tgttcggcgg cgcggagcgc gtggcgcagc
     3841 aactcttctg tcaccgtttg ctgaaaagag gcgatcaagt ccagcgtcga ctttggcgta
     3901 atctccagcc actgctcgac gctcggccgc ggattctcgc gcagcagcgc ccggcggcgc
     3961 tggatttcct ctgccatccc ggcggcctgc acatggcgca gcacggccgt cttgagtccg
     4021 ctgaaactga aatccagatc gttgcccttc attttcgcgc gggtgaagtg gattgcctcg
     4081 ggatcgccgt atggcgccaa acggtcgatt acaggtccgc cgggatagcc caggcccagc
     4141 agcttggcga ctttgtcgaa agcctccccg gccgcgtcgt cgcgcgtgcg gccgataagg
     4201 cggtagtcat attccgagcc gtcccgcctg acatagtaca tatgggtgtg accgccgctc
     4261 accaccagag cgagcgccgg aaactcgatt tcttgctgct tttccctaag ttccagaagg
     4321 acggcgtgaa tgtggccctc tatgtgattc acgcctatca gcgggatgtc gaaggagagg
     4381 ctcagcgcct ttccgtacac cagccccacg agaagagacc caaccaggcc cggaccacgc
     4441 gtcacggcga tcgcgcctaa atcgcggtac gagaggccgg catcctcaac ggcctggcga
     4501 accaccggca caatcgcccg gagatgttct cgggacgcca attcgggcac aacgccgccg
     4561 taccgccggt gcgtgtcgaa ttgcgaagca atgatgttgg aaagcaggcg ctcgccggac
     4621 tcaactaccg ccgcggcagt ttcatcgcac gagctttcga tgccaagaag gaacatccgt
     4681 gccgcaccgc caggctctca gcgttcagct tttctggctc cctgccggct gctgacggct
     4741 ttttttctcc agaaagcaag cccccggaaa gccgtcaact ttccaggggc tcacaaggta
     4801 gcccaggatt ctttatcctg aggctttcgt gttttatacc gaactgccac ctgtgaaatg
     4861 gccgttcagc aggaactggc cccgaccact gacatcgggg aaccgaacgt tagagcaacc
     4921 gactcaggag tcggccacct cacagggtct atgactacac atattgtctg gaccacctcc
     4981 ttcctcagtg ataacttcat cctaagagcg gcccccggca aagtcaagaa aaacgtatgt
     5041 gggagcatct ttcggcagcg cccgcggccc gcttgccgcg cctttcagct caggcatatc
     5101 tcccagtctg cgggggcgcc tatcgcgcgt ttctccggtc gcgccggcct gaacctggtc
     5161 ccgccagggc aacacccagc cttaagaacg cagcatcctg ccggccgaaa aggggcatgt
     5221 cgaggcaatg ctacactgat ctgcgtggtt ccggccattc tcacacgagg cctgcgaaag
     5281 ctgtatggga acaaagccgc cgtggacggt ctcgatctgg aggtcccgca aggctccttt
     5341 tttggttttc tggggccgaa tggcgcgggc aagaccacca cgattcatat cctcatcggt
     5401 ttgattccgc ccacctcggg cgaggtccag gtcctcggcc tgccgctcgc ctcccacttg
     5461 caggagatta agcggcgcat cggtgtggtt cccgacgaaa acgtgctgtt tgaccgcttg
     5521 acgggatttg agtttctcca atttgccggc cgcatgtacg gcctggaacg gaatgtggcc
     5581 cgcgcgcgcg ctcgcgagct gctggaactt ttcgagctcg atcaggaacc tcgcaagctg
     5641 attgccgagt actccaaggg gatgaagaag cgcatcgcct tggccggggc cctgattcac
     5701 cggccgcagt tgtttttgct cgacgagcct ttcgagggcg ttgacccaat gggggcgcgg
     5761 ctgatgaagg aaattctact cggccaggtc cggcaagggg ccacgatctt cctcacctcg
     5821 cacgttctgg aggtggttga gcggctctgc gatcgcgtgg ccattatcaa tgaaggcaga
     5881 attgtaatcg aagggtccat gcgcgagctg cggtcggcgg aagacacact ggaggacaca
     5941 tttgtgcgcg tcgtgggcgg cgaacgcgca gcccagagcc tcgattggct gtgaccccgc
     6001 cgtgagattg tccgcgctgg tccactggcc gtttcgcgtc ctcatggcgc gctctccggc
     6061 agggcagtca ggctggaaat taccaatgat ggcggcggaa gcaacccggc aaatccaggc
     6121 ggtgcttcac atccaatggt tgtctttcag gaacggcctg cgccgccgtt ccgagcgcat
     6181 cgggctgctc ttttcgattt ttgtcactac gatctggtat gcgatttggc tagcagccgc
     6241 cgctgggctg tgcctgtttt cgctttgggc atccaccggg cggctctcgc ggaccctgcc
     6301 tgccctgctc ctgctgttgc tggtttactg gcaagccagt cctctactca cggcctcgat
     6361 gggcgtatcc atcgatctgc gcaaaatggc gctgtatccg ctcgatgtga aaacgctttt
     6421 ccttatggag tgcctgctgc gcttgatgac atcggccgaa atgatcctgg tgctatcggg
     6481 gctcacggtt gggatggcga tgcgagcgcc ccagcaaatt ccgatatttc ttcctgcggc
     6541 ggcgctgttc gtcttattta gcgtgctcct ctccgccggc ctcagaaacc tgttggaacg
     6601 gctcttgcag cggcgggggt tccgcgaaat gtttctcttc ctgatcgtga tggccagcgc
     6661 gactccgcaa ttcatcctgt ggaccggctc tggggagaaa gttggacgcc gcgtttaccg
     6721 gattttccat tccctgcccc aattcatgct tccatcgact ttcttggcgg aggcgtattt
     6781 agggcgcagc acgttttcga actggattct catgatcggc tggtgcacgg cggcggccag
     6841 ctttggtttt tggcagttcc gtcgaagctt ttaccccgat tttggctctg gcggggcgaa
     6901 ccatgcgccg cctttctcgc gctccgacga ccgggaaagg ccgctgaacc gttggcctgg
     6961 cttggttgcc cgccttctgc cggacccctg ggccgctctg gtggaaaaag aggtttgcta
     7021 tctttcccgc agcccccgct tccggtttct ttttctgatg ggatgttctt tcggagtagt
     7081 agcttggctg cctttcggga tgagccgcgg cccactcgca accggtgttt tgcaggcaag
     7141 ctttctcact gtcctcagcc tttatgctct gctgctgctc gggcaggtta cgtttttcaa
     7201 ctccttcggt ttcgaccgcg ccgcgacccg ctatttcttc tggagcccgg tttcgccggc
     7261 ggccgtgctg gcagcgaaaa acctggcggc agtggctttt ttggtgctcg agatttttgc
     7321 tctctcctcc atatgccttt tgcttcggat gcttaacggc ttttggcagt ttgccgaagc
     7381 tctggcagtg acattgatct ccagcttcta ccttctttca gtggggaatc taacctcgat
     7441 cctgttccca gcggcgctca gcccggagcg ggtctcccga gctggctccg gccgcggcgt
     7501 tcagggcttg atcgtgtttt tgtacccgct actgattttt cccgttctcg cagcctactt
     7561 cgcgcgatac tactggcaca gcagaaacgg cttcctcctg cttctcgctg cggcagccgc
     7621 cggtggtctc gccctctacc tggcgactct gcccctggcc gcccggttag cgtttctccg
     7681 gcgggaaaag ctgctccagg acctgagccg cggcgaaggg ccgctggtca gtgagtaaag
     7741 cggggcgtcg tatcccagcg aaagcgttca ataatctctt ccaccggccc tgacgcggcg
     7801 atttggccgt tctgaaaaaa aatggcgcgc gtggcaagtt cgcgggccag atcgacatca
     7861 tgactgatga taagtttagc ttgcggcagc cgccccagaa tgtccaccaa cgcggcgcgc
     7921 cccggcggat cgagaaacgt caccggctca tccaggaaca agatttccgg ttgcatggcc
     7981 agcacgcccg ccagcgcggc gcggcgtttc tcgccggcgc tcaaatggta tggcgctttg
     8041 ccggccgcag cttccatgtc tacgagccgc aggcattccc gcacccggcg gtctacttcg
     8101 gccgcttcca gccccaggtt cagaggcccg aacgcgacat cgtcataaac agtaggcatg
     8161 aagagctgat cgtcggaatc ctgaaataaa atgccggcct tctggcgtat ggcggggaag
     8221 ttcttgcgct ccgcgggcag cccgcaaatt tcgatccggc cctcgctttc cagaagccct
     8281 gacaggcata gcaagaacgt ggttttgccc gagccgttcg gcccgaatac ggcgactgtt
     8341 tccccgggat aaaggacaaa gtcaacacct cgcagcgcct gtgtgccgtc atcgtatcga
     8401 tggctgagat tttccacccg gatcagcggt tccataagag aacctggagc gcggcgatca
     8461 cggcgcccgt aactgacaag aacactaggt cagccgctcg caaggagggt ggccgtgcgg
     8521 ctgcgaactg cccctggaac cctctcgcca gcatcgcgtg atggattctt tcggcgcggc
     8581 aatagctgcg ggcgaagagc actgccagcg cgccggctgc cgacgccccc aggctccggc
     8641 cccctcgccc gccctggcct gcacggcagc gccgcgcctg cttcatatgc tgcgcctgtt
     8701 ctgacaggac aaacaggtag cgatatacga actgcgcgac catcaggaag aaccgaggcg
     8761 cccccagcga ctccaagccg cggagcagga cgtgcagcgg cgtggacgcc agcagcacca
     8821 gcacggccag tgcggaaaga tagcttttcc cgagcacggc cgcggcgcga acggccgttc
     8881 ctccgaacac attcaaagcg gcgacggtgg ccgcgaaagg cagcaccagg gccgcccgca
     8941 gcagcagcgc cgccaggggc agccgtgcca gccatgctaa ggtgaagatc agcgagaagt
     9001 acgaggcgaa aagccaaaga taggcgtgtt cggtaagcac tagagacagc aggacggcga
     9061 ggaccgcgag aatcttcact cgggcgtcca accggtgaat ggggcttccg atgcggctcc
     9121 aactatcgag tgttacgtgg tgcatttcgt ctcttgcgtt tcagagtccg gccaaaaagc
     9181 acgccgaacc cgtagagcaa tgccgccccc agcagcccgg ccgatgcctg cgccggccat
     9241 cgggagtgga aaaaccgcgc ctcgtagttc gcgaaaggag atgccagacc ggcatttctg
     9301 gtgcgcgagg cgaatcccag ggtggcggcc acccgctcaa gaccatcggg gaaagtggag
     9361 gagataagga ccccggccgc caccaggcct agggtggcaa agccgaaggc caggaccagc
     9421 cgcctcatcg ttcaactctc catctgctcg ccgcgaaaaa taaacctcgg gctcagccgc
     9481 gcaatcgcct ccagcacagc caccgtgatc acgccctcga tgaatccggt caccgcgtgg
     9541 atccccagca tcgaggcgaa cagcggccgg ttcggggcga cgtgcgaaag gctgagttcg
     9601 accgtggcca gcagcgcggc gccgaataca gaaagccagc cgccgagaaa aaccgcggcc
     9661 ctctgccgcg cagcgccgcc gccgatcagc ttaactgccc agtggccgag ccacaccccc
     9721 gccaaggcca tgtttaagat gttggcgcct aaagcggtga ttcctccgtc ctgaaatacg
     9781 agcgcttgca caaccaaaat cgccgtcatc accacagtag ccgcccatgg ccccagtgta
     9841 agcgccagca gcgctccacc caccagatgc ccggaagtcc ctgcgcccac cggaaagttg
     9901 atcatctggg cggcgaaaac aaacgcaccc atgaccccga gcaacggcac tcgggtctca
     9961 tcaaaacgcg cctgcgtgcg gcggacgaag tagccgatca cgggcgccgc ggcggcatcc
    10021 aggcttaccc aaaccggcgt ggacaaaaac ccgtctggaa tgtgcatggg atcccaagcc
    10081 tcctacggta gcacgattca aaaattcgtg acaccgcgaa aagaatgccc cggcggtctg
    10141 aaggcgccgg gccgagggga agatccggct cttggccgtg agttgcgggt accggcggct
    10201 cctgcgggcg cttccctggg ctgggcaaaa aagcgcccca agattgcggc cctggcgcgt
    10261 tttgtggcct gcgcctccgc cggcccttgc tacgctaaaa tcgggccgtg gagcctatcg
    10321 tcgagctgcg ggaagtcgta aaatccttcg gcgctcgcgt tgcggtcaat cacgtttccc
    10381 ttgccattcc gcgcgggcgg ttttattccc tgctgggtcc ttccggctgc ggcaagacaa
    10441 cgacgctgcg gctgatcgcg ggctttgacc ggcccgactc gggagacgtg ctcattcacg
    10501 gcgtccgggt caacgacctc cctccctacc ggcggaaagt gagtacggtt tttcaaagct
    10561 atgccctttt cccgcacctc acggtggcgg gcaatgttgc cttcgggctg aaacaggccg
    10621 gcctgccgaa agataaaatc cgcgcccgcg tccaggaggc gctggagctg gttcagctct
    10681 ccaaattcgc tcacagcctg ccgcgtttcc tctccggcgg agaggcgcag cgggttgcgc
    10741 tggcgcggtc gatcgtcctc gagccggatg tgctgttgct cgacgagcct ctcgcggcgc
    10801 tggatcaacg gctgcggcgc gagatgcgcg tggagctcaa acgcttacag agggccgtcg
    10861 gcattacgtt ccttttcgtc acgcatgacc aggaggaagc tctcacgctc tctgacggca
    10921 tcgcggtgat gaacagcgga aaattggaac aggtggggac gcctgaggag atctatcgct
    10981 cgccacgctc ccgcttcgtc gcgagcttcg tcggcagctc gaatctgatc gaagggagag
    11041 tcacccgcgt cgagcagggg cgcctgattc tcaccacgcc cgccgggcgc tgcttctctg
    11101 ttccctgccc gccgtcgccg cctccggtgg gctcgctcgc cggactcctg gtgcgttcgg
    11161 aggccattca gatcgccaac gggtctgctc cttctcaagc acaaaggctc accggaaaag
    11221 tagtgcaatc gatgttcctc ggcccttatc gccagatcgt cgtgcaagtg gacccggaaa
    11281 tcgaactgac cgtgctgtgt ccagcagcta cgcccctccc ggccgatgcg agcctcgagt
    11341 tgtggtggaa tccggaagac tgcctgatct tgccgggttg atcgaatttt cgcagacgcg
    11401 ggcgcgctgc cagcgcgcta caggcaacaa agaagcggcg cgcaaagctg cccccggttg
    11461 tgtttgaggg tttacgggcg atagccgggg ccgcgcagca ctttgcccgg ccgggccccg
    11521 gtcatcttgc ccccgtcgat caccggtgtg ccgttcacca gcacgtactg catgccctcg
    11581 gataaccgat tgggattttc aaacgtcgcc agatcccgga tcttgtctgg gtcaaaaacc
    11641 acgatatcgg cccagaggcc ggacttcagg aggccgcggt cggcaagccg catttgcccc
    11701 gccggcagcg cgctgaactt ccgaatcgcg tcctccagcg tgagcttttt ctcctcgcgc
    11761 acgtatttac gcagaatgcg gggaaaggtc ccgtaggcgc gcgggtgggg atgtttcttg
    11821 cccagtatgc cctcgggcga cgtgccctgg gagtcattgt cgatcgagac ccacggttgc
    11881 tgcagcgcca gcgtcacatc cggctcagac atgccgaata ctgcgacata ggtataagcg
    11941 ttgtcttcga ccagcaagtc aaagatcgtc tcaatggggt ctttacccca ttgcccggcg
    12001 atctcggcga ttgtttttcc ttgaagaggg agcagcttcg ggttctgtac gacgcaaacc
    12061 tggattgcct cgggacctgg aatggcctgc cactcgttat cccaggcatc gctcggcgtc
    12121 tccagatcct tacggatgcg cgcccgcgcg gcagggtctt tcagccgcgc gatcagcttc
    12181 gcgtcgcccc catcatgcgc ccagggaggg atgaacgccg agaaagagtt gaaccacgcg
    12241 gtatatgcgt acgtgttggc gccgatctcg acgccggacc ggcgggcttt ctcgatatgc
    12301 tcgatgatct ccggcatccg cccccagttc gatttgccgg ccgccttcaa gtgccagatc
    12361 tggaccggaa tttttgcttc ccgcccgatc cggatggctt cgtccagggc ggcaatgacg
    12421 gcgttgccct cgctgcgcat gtgtgtggcg tacacaccgc cgaattttgc cgcttccgcc
    12481 gccagcgcaa tcagttcttc ggtggttgca taaggagcgg gagcgtattg cagcgaggta
    12541 gaaagaccga ttgccccctg ctgcatggcg tcgcgaacca gcgacttcat acgttccagt
    12601 tccgccggag acggcggccg gtcatcctcg ccgatgacca tgcgccgcac cgtggtagcg
    12661 ccgacataac tggccaggtt gatcccgatg ccttgccttt ccagccgcgc aaaatactgc
    12721 tggaaattcc gccagtccgg gacgattttc aggtgctcga agctgaggcg gtcagcgcgc
    12781 acgatggcgt cattgagggg ggcgactgag ttcccctccc cggtgatctc cgtagtaatg
    12841 ccctggtaaa tcttcgatgg cagccgcggg tccaccaaaa cagtcagctc cgactgcccg
    12901 agcatatcga taaagcccgg cgcgaccacc atgcgctggg cgtcaatgac gcgtttagct
    12961 tgcgcctccg ccagatttcc aatggaggcg attcggccgt cgcggatgcc aacgtcggcg
    13021 gcataccagg gtgagccgga gccgtcgatg acatggccgt tgcggataat gatatcgtac
    13081 gtctgggctt tggactcggc cagaaagaaa ccgaagaagc cgcacagcag ccagagagag
    13141 cgcgccagcc gcgtccagcg ggaagtatag agtgcgagcg ggcctgctat tgtgcgcgat
    13201 ccaaactcgc cagcattcat cccccgtcct cgattttttc ggagatcact ttggcctgca
    13261 tgaacaacag gagataatcc ctgccgcctg ctttggagtc cgtgccggac atgttgaatc
    13321 caccgaacgg gtgccctccc accagcgcgc ccgtgcactt ccggttgaga tacaggttgc
    13381 cgacataaaa gctctcggcg gctttcttta gcttttcgcg atccctcgta tagacggcgc
    13441 cggtcaggcc atattcgctg tcattggcca tttcgagagc ctggtcgaaa tcgcgggccc
    13501 gcgtaaccgc cagcacgggg ccgaagatct cctcgcggaa gatacgggcc cgcggctcga
    13561 catcggcgat gagcgtgggc tcgaggaaat agccgtggcc gggcgcccgg cggccgccgg
    13621 tgagcagttg gccttcccgc cggcctgtct caatgtaatc gagaatcgag cgcagcgccc
    13681 gctcgttgat gaccggcccc atataattcc gtgggtcttc gggcggccca acggtaatct
    13741 gtcgagttct ttcgacgagc cgctccagaa acttgtcgta cacggcatcg acgacaatcg
    13801 cccgcgagca ggccgagcac ttctgtccct gatagccgaa ggcggaaacg agcacgccgg
    13861 caaccgcttt gtcgaggtcg cagtcggcat ccacgacaat tgcatccttg ccgcccattt
    13921 cggccaccac gcgcttgatc caccgctggc cgggctgggt ttttgcggcg agttcattga
    13981 tgtgcagccc gacctgtttc gagccggtaa aagcgacgaa acgcgtgagc gggtgctcga
    14041 ccagcgcgtc gccgatgctc gaaccgctcc cggtaaccag cgaaattgct tccggggggc
    14101 aacccgcttc cagcagagct tccaccagat gcgctgcaat ggtgggggaa tcgctcgaag
    14161 gtttcagcac caccgtgttg ccgctcacca gggccgccgt tgtcatcccc gtggcgatgg
    14221 ccagcgggaa attccaaggt ggaatcacca cgcccgcccc gagcggcagg taccgaagct
    14281 cattgcgttc tccggggacg ggcgtgagcg gctgcggccc gctcaggcgc agggcctccc
    14341 gcgcataaaa ctcgcagaaa tcgatcgcct cggcgacatc ggcgtcggct tccgcccacg
    14401 gcttaccgac ttcataaatc atccaggcgg aaaattcatg gcggcggcgc cgaaagatgc
    14461 gcgccgtttc cagcagcacg cgggcgcgct gctcaacggg ctgggcgctc cagcaacgaa
    14521 aatagtcgtt cgccgccgca atgctgcgcc gcgccagctc gacgtcggcc gccgcgtgca
    14581 cacccacgat ctgctcgggg tgggccgggt tgatagagcg aatcttgttt tctgtcgaga
    14641 cccgctgtcc gccgatgagc aacgggtatt cgcggcccaa ctgcgcccgc accttctcca
    14701 gcgccgtttc catcgcagcg cggttttctg tcgccgaaaa gtcggtcagc ggttcgttct
    14761 ggaattccga gagctgcata ctccctgccc ggcgccgcgg acgagcaggc agccggcgca
    14821 tattgcgcat tccaggcgcg gcgcccactt cgagaaccat tcccgttgcc cggctggcct
    14881 gcccggagtg tttacagtct gactgccttg cggccgcgct caaaaagctc ggcggcgtca
    14941 tcggcgaggt ccttgccctt ttcgaaaatc tcccggcccc gatcgtagat ttcgcgcccg
    15001 gcgtccacca ggcggtcttt cccttctgct gttttctccc ccagcaattg ccgaagctcg
    15061 cggcctgtct tgggagcgaa caacagaccc acggccgcgc cgatcgccac tccagccaaa
    15121 aaccacatca ccttttcgcc gccgtctcca cgcgccatgt gttttctcct atatggattg
    15181 taaacctttg cccccgcgcg cag
//
LOCUS       DASREK010000010         5197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1817007, whole genome shotgun sequence.
ACCESSION   DASREK010000010 DASREK010000000
VERSION     DASREK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817007"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /gene="bamE"
                     /locus_tag="VFA54_00705"
     CDS             <1..232
                     /gene="bamE"
                     /locus_tag="VFA54_00705"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZS49350.1"
                     /translation="SQAQFQAKLKGGGGFELSIGMTQDQVRALWGPPARWRSSNDYLQ
                     WTYINKGRDEAGRLCNVVVVFSNGQVVTWNND"
     gene            complement(454..1569)
                     /locus_tag="VFA54_00710"
     CDS             complement(454..1569)
                     /locus_tag="VFA54_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZS49351.1"
                     /translation="MKFTLSCFFSAAVFAASLPLARAAGGANVYYANTGQFSAMFVAD
                     TDEAGAAQADTAKERAVNENEALRQQNENLRRQSELLKEQAARIDGPISLVARGSLEN
                     VAFIGVETSPVTPALTAQLALPEGTGLVVEQVVPDSPAAASIKPNDILLKLDDQWLID
                     PWQFAVLVRQHKAGDDVVLTYVRGGKQATVSIKLAEHQAPKLSDLQSSRGSRALMLGI
                     APGMPGPDPDRMLGLVEQMRRKSDGENFLVNRVPGTERSRLRTVQVDAANSNFAYSDD
                     DGTLTLTVKDGKKTLVAKDAQGKELFSGPVDTPEQRQAMPEAVRARLEKVEKMHGVSF
                     KTGPDFKVQTRENHPDGEDIVFPLPSPRAAPLPSEAF"
     gene            complement(1595..2038)
                     /locus_tag="VFA54_00715"
     CDS             complement(1595..2038)
                     /locus_tag="VFA54_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49352.1"
                     /translation="MDDDLQKFEAELKALRPAVPSRELMGALERRLAPRRMGGLFWLG
                     LPAAAAACLLLVLHFRPEPAANRQPPATASMTETAPALKPVAADDVLVDTQDEGFVTL
                     ADGTQARRTRQSFVDTITWEDPATHASLRWSVPREEVNVLPVNFQ"
     gene            complement(2031..2576)
                     /locus_tag="VFA54_00720"
     CDS             complement(2031..2576)
                     /locus_tag="VFA54_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS49353.1"
                     /translation="MTLRGKTTPLARMDDAPNDDSWKNWFQRYGPRLLLCARQWTRSL
                     ADAEDVVQDAFIRFWRRQRGLGGEPLPLLLTSIRRAAFDRARREQRRAAREQETEAGE
                     SAPLFEPLAVGDERRMAVEAALRRLPAEQREVLALKIWSNLTFEEIGGQLEISPNTAA
                     SRYRYALAALRKELTPDFCHG"
     gene            complement(2769..4280)
                     /locus_tag="VFA54_00725"
     CDS             complement(2769..4280)
                     /locus_tag="VFA54_00725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS49354.1"
                     /translation="MPASRLVWALAGMLLLLHLLTAGRYGIFRDEMYGLACARHLDWG
                     YVDHPPGGIVITWIAHHLFGDSLQGLRLLPALAGAVLVLLTGRLVRELGGGGFAQVMA
                     ALAVCFAPYYLILDHLLMMNAFEPLIWMGCLYCVIRATRPGGTNHWFGFGVLAGIGFE
                     TKYSIAFLVVGVLVGLALSPQRRQMADWRLWAGLAICGAIALPNFLWQWRHGFPFLEF
                     RHNVARNHRDIVRGPVAFILDQMVVMGPLSAPLWIIGAIWLLAGRRRAQYAVLGWTFL
                     VVLGLFIALKGKNYYVSPVYPIVFAGGAVALEQFTEGERRRWLRVVYPAILAAVGLVL
                     LPLAVPILPPEALVRYEQRLGIKPPEFEHQKTGLLPQYFADEFGWEEMVREVARIYHT
                     LPPEEQGRTLIFSNSWGAASAVDFFGPKYGLPHAVSKNNSYWLWGPGDHPGDIVIVLH
                     TDGTGDRKFFASVEKAGHVEHPWSRRDEWYDIYVCRGPKFDFRTAWSKMKTFD"
     gene            4624..>5197
                     /locus_tag="VFA54_00730"
     CDS             4624..>5197
                     /locus_tag="VFA54_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZS49355.1"
                     /translation="MLRSVGFDVRRCAPSDRGIDPFYDMQHFLRGVPSPLILDVGANV
                     GQSVDRFKHVFPDSFIHSFEPSPATYTILEQHCNRIGGVKTWNYGVGSSAGALPLLEN
                     SESDLSSFLAPGSYCWGEVTRTTNVEVVTLDAFAQAQSLGFIHVLKSDTQGFDFEVLK
                     GAERLMNEDQIGLIYFELIVSDQYERLPPFT"
BASE COUNT         1031 a         1681 c         1543 g          942 t
ORIGIN      
        1 gagccaggct caatttcagg ctaaacttaa gggcggcgga ggttttgaac tttcgatcgg
       61 catgacccag gaccaggtcc gcgccctctg gggtcccccc gcccgttggc gctcctcgaa
      121 tgactacctg cagtggacct acatcaacaa gggccgcgat gaggccggcc gcctctgcaa
      181 tgtcgtcgtt gttttttcga acggccaggt cgtcacgtgg aataacgatt aagcgcggcg
      241 ggtgccagtc cgcgcggaaa gatcagggaa ccgtcggctg atccggatga tttcacagcc
      301 cgttccatcg aacagcttgg ccggcgagga atattctcac gcggttcgga ccggctgcgg
      361 ctgcgtttcc aaaaataatt caaaagccgc gtagcctgac agccacgcgg cctttgatta
      421 accctaaaca ctatgctaac cgaaaattgg ctgttaaaac gcctcggacg ggagcggtgc
      481 cgcccggggt gaaggcagcg ggaagacgat atcctcgcca tccggatgat tctcccgcgt
      541 ctgcaccttg aaatccggcc cggtcttgaa gcttacgccg tgcattttct ccactttctc
      601 cagccgtgcg cgcaccgcct caggcatcgc ctgccgctgc tccggcgtat ccaccggtcc
      661 ggaaaatagc tccttcccct gggcgtcctt ggccacgagc gtcttcttgc cgtccttgac
      721 cgtgagagtg agggtgccgt cgtcatccga ataagcgaag ttgctgttgg ccgcatcgac
      781 ctggaccgtt ctcagcctgc tccgctcggt gccgggaaca cggttgacca ggaaattttc
      841 tccgtcgctt ttgcggcgca tttgctcgac cagtccgagc atacggtcgg gatcgggtcc
      901 cggcattccg ggggcgatgc ccagcatcag cgcgcgcgat ccgcggctcg actgcagatc
      961 ggacagcttg ggcgcctgat gttctgccag cttgatcgag accgtggcct gcttgccgcc
     1021 gcgcacgtag gtcagcacca cgtcgtcgcc ggctttgtgc tggcgcacca acaccgcgaa
     1081 ctgccatgga tcgatcagcc attggtcgtc gagcttgagc agaatatcat tcggcttgat
     1141 cgatgctgcc gccgggctgt ccggcaccac ctgttccacc accaggcccg tgccctccgg
     1201 cagcgccagc tgcgccgtaa gcgcgggcgt gaccggcgat gtttccacgc cgataaaggc
     1261 gacattttcc aatgacccgc gggcaactag tgagatcggg ccgtctatcc tggcggcttg
     1321 ttccttcagc aactccgatt gccgtctgag gttctcgttt tgctgtctga gggcctcgtt
     1381 ctcgttgacg gccctttctt tcgcagtgtc ggcctgtgcg gcgccagctt cgtccgtgtc
     1441 ggcgacgaac atggcggaga attggcccgt attggcataa taaacattgg ccccgccggc
     1501 cgcgcgagcg agcggaagac tcgccgcaaa aacggccgcg gaaaagaaac aagagagggt
     1561 gaatttcatg gttgggaatg ttgttggaaa aaattcattg gaagttaacc ggcaggacgt
     1621 tcacctcctc gcgcggcacg ctccagcgca acgaagcgtg agtcgccgga tcttcccagg
     1681 taatggtgtc gacaaaggat tggcgcgtac gccgggcctg ggtgccgtcc gccagcgtca
     1741 cgaagccttc gtcctgcgta tcgacaagca cgtcgtcggc cgccaccggc tttaacgccg
     1801 gcgcggtctc ggtcatcgag gccgtcgccg gcggttgcct gtttgcggcc ggttccggcc
     1861 gaaaatggag cacgagaagc aggcacgccg cggccgctgc cggcagaccg agccagaaaa
     1921 gcccgcccat ccgccgcggc gcgagccggc gctcaagcgc gcccatcagt tcgcgcgagg
     1981 gaacggcggg gcgcagggcc ttcaattccg cttcaaactt ttgcaggtcg tcatccatgg
     2041 cagaagtccg gcgtcagttc cttgcgcagc gccgccaggg catagcggta gcgcgacgcc
     2101 gcggtgttgg gcgaaatttc cagctgcccg ccgatttcct caaaggtgag attgctccaa
     2161 atcttgagcg cgaggacctc gcgctgctcg gccggcaggc gccgcagcgc cgcctcgacc
     2221 gccatgcgcc gctcgtcgcc caccgccaaa ggctcaaaca atggagcaga ctcgcccgcc
     2281 tcggtctctt gctcgcgcgc ggcacgccgc tgttcccgcc gggcccggtc gaatgcggcg
     2341 cggcggatgg acgtgagcag aagcggcagt ggctcgccgc ccaacccgcg ctgccgccgc
     2401 cagaaacgga taaatgcatc ctggaccacg tcctcggcgt cggccaggga ccgcgtccat
     2461 tgccgggcgc agagcagcag tctcgggcca taacgctgaa accagttctt ccaggagtca
     2521 tcattgggag cgtcgtccat gcgggcgagc ggagttgttt tccctctaag cgtcatcgga
     2581 cccgaaattt gtctaaacga tgatgacctg acgtcaaaat cggcgttcca atggaacatc
     2641 ccaggcgttc cccatcagtg aaataagcgc ccactcgcgc tggcggacgg actctttcta
     2701 ttcgcggcac ggaggtcggc cgccggcgca acgccgtttg agttccggcc cccctctttg
     2761 ccaccatatc agtcgaacgt cttcatcttc gaccacgccg tccgaaaatc gaacttcgga
     2821 ccgcggcaga catagatgtc ataccattcg tcgcggcggg accaggggtg ttcgacatga
     2881 ccggcttttt ctaccgacgc aaaaaatttc cgatcgccgg taccatcggt gtgcagcacg
     2941 atcacaatgt cgccgggatg atcgcccggc ccccacaacc agtagctgtt gtttttgctg
     3001 acggcgtgcg gcagcccgta cttcggccca aaaaaatcga ccgccgaggc cgcgccccaa
     3061 ctgttggaaa aaatcagcgt ccgcccctgc tcttccggcg gcagcgtgtg gtagatccgc
     3121 gccacttcac ggaccatctc ctcccagccg aactcgtcgg cgaaatattg cggcagcagt
     3181 ccggtctttt gatgctcgaa ctccggcggt tttattccca gccgctgctc gtaccgcacc
     3241 aacgcctccg gcggaagaat ggggacggcg agcggcaata aaaccaatcc cacggcggca
     3301 agaatcgccg gatacaccac gcgcagccag cgccgcctct caccttcggt aaactgctcg
     3361 agcgccaccg cgccgccggc gaaaacgatc ggatagaccg gcgaaacgta ataatttttc
     3421 cctttgagcg cgataaacaa cccgagcacg acgaggaatg tccagcccaa cacggcgtat
     3481 tgcgcccgcc ggcggccggc cagaagccag attgctccga tgatccaaag tggcgccgac
     3541 agcggcccca tcacgaccat ttgatcgagg atgaacgcca ccggcccgcg cacgatgtca
     3601 cggtggttgc gcgcgacatt gtggcggaat tcaaggaacg gaaagccgtg ccgccactgc
     3661 cagagaaaat tcggcagcgc aatcgcgcca caaattgcca gcccggccca aagccgccaa
     3721 tcggccatct gccggcgctg cggactgagc gccaggccga cgagcacacc gacgaccaga
     3781 aacgcgatcg aatacttcgt ctcgaacccg attcctgcca gcacgccaaa gccgaaccag
     3841 tggtttgtgc cgcccggccg ggtggcgcgg atcacgcagt acaggcagcc catccagatc
     3901 aacggctcaa atgcgttcat catcagcaga tggtccagga tcaggtaata gggcgcgaaa
     3961 cacaccgcca acgccgccat gacctgggca aacccgcccc cgcccagttc ccggacgagc
     4021 cggccggtca gcagcacgag cacggctccc gccagcgcgg gcaacaggcg caacccctgc
     4081 agggaatcgc cgaacagatg gtgcgcgatc caggtgatca caattccgcc cggcgggtgg
     4141 tcgacgtaac cccagtcgag atgccgggcg caggcgagcc catacatttc atcgcggaaa
     4201 atgccatatc gtcccgccgt cagcaggtgc agcagcagga gcatgcccgc cagggcccag
     4261 acaagtcgcg acgcaggcat gactgaccgt tcagccaagg ggtccgattc gggtcgaagc
     4321 atgggcggag tattggggcg ccagaccgtc cgcgccaacc cggtttttcc gatggcaacc
     4381 ctgccaagct ggccggaatt cggcctcagc ccggcatctg gctgaagcac aaaagtcccg
     4441 cgaaaactga aaaccaccgg tcgtgagcct gctcacccgg cgacccccga aaaagaatat
     4501 ccggtcgtcg cctgaatttc gtaacagggc ggaacaattc ccgtctccgc gcaaccaagc
     4561 tgccccgcgg gatcacggca tcgtttggtg gtcatgaaaa acatgaagca atcggttcgt
     4621 ggaatgcttc ggagcgttgg cttcgatgtc cgccgctgcg ccccgagcga ccggggcatc
     4681 gatccgtttt acgacatgca gcacttcctc aggggcgtgc cgtcgccgct tatcctcgat
     4741 gtaggcgcga atgtcggaca atccgtggac cggtttaagc acgtgtttcc cgactcattc
     4801 atccactcct tcgagccgag cccggcgacc tacacgattt tggaacagca ttgcaaccgg
     4861 atcgggggag taaagacatg gaattacggc gtcggctcga gcgccggcgc gctgccgctc
     4921 ctggaaaata gcgagtcgga cctgagctca tttctggctc cggggtctta ctgctggggc
     4981 gaggtgacaa ggacgacaaa tgtcgaggtg gtgaccctgg acgctttcgc ccaggcgcag
     5041 agcctcggat tcattcacgt cctgaaatcg gacacgcagg gtttcgattt cgaagttctg
     5101 aagggcgcgg aaaggctgat gaatgaggat caaatcggcc tgatctactt tgagctgatt
     5161 gtatccgacc agtacgagag gctgccgccc ttcaccg
//
LOCUS       DASREK010000011        17752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_499901, whole genome shotgun sequence.
ACCESSION   DASREK010000011 DASREK010000000
VERSION     DASREK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17752
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499901"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VFA54_00735"
     CDS             <1..1217
                     /locus_tag="VFA54_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HZS49356.1"
                     /translation="TGAYANTRPLWRHTSKSPSEATLWMAQTTASGMAPWYHWLGGAP
                     EDLRWRETGRRFYQWHARHEKHFVNRRSIANLGVVFSERTNAFYHPPGGDDPTEFLQG
                     LYYALLEGRFFFDFVHEDDLVPETLKKYKALLLANVAVLSDEQCRQLRDYVQSGGSLL
                     ATFETGRYDDRGQARSGLGVEEIFEMRPKGRVQGPNGNAFYARIEQSHEILNGFENTK
                     LLPGAEYRQPVEAAGGAVLTVVPPFPAFPPEMVYANTPHTDEPAIVIREKAASRLIYL
                     SGDADRSCWRSGNPDLSLLLGNCVRWLLRGSPVEVAGEGMAELFAWETEPGLAVHVIN
                     YNNPNMMRGWMRRNYPLGPQRVRMELPAGFHVSRVQALRAEINLPYRQSGRRLEFTIP
                     KVEDYEVAALSA"
     gene            1648..5073
                     /locus_tag="VFA54_00740"
     CDS             1648..5073
                     /locus_tag="VFA54_00740"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS49357.1"
                     /translation="MAWQNVANFLGIKGGFMGYPFWCPWRVLSLSLILVWATTARAQT
                     QGQITGVVTDPSGALVSGATVTVVNPQTGFTRQVRTNTAGNYNFPALLPGLYDVKVEM
                     QGFQTEIRSGVELQVQQVARIDFALRVGAVAETVEVTGGAPLLNTENATVGTVIENQR
                     IVELPLNGRNFLQLVALSPNVNASFASAGQSGSRQGGDRANQQLSISGNRREWNYFTL
                     DGVDNTDVNFNTYTFLPSIDALQEFKVQTGIYSAEFGRETGQVNVSTKGGTNQYHGAL
                     FEFLRNSSLDARPFAFTSAVPTKAPFRWNQYGFTLGGPVQIPKLFNGKDRLFFMSNFE
                     GFRLREQRQGIYSVPSAAMRNGDFSEILPTPINDPLTNQPFPNNKIPSARLHPIALKL
                     LEFYPPPNVPGAGLQRNYLALQNNRTDKDQFTQRIDFVESAKSSWFGRYSWGDEQAIA
                     PALKLNGTNLQTHVNQWMIDNTRVLRPNIVNEFRFGYSHFFNSFGRELAFQRDVVKEL
                     GLTAVGLNDPPPVAWGIPAISITGFSGFGDDSEGPYVNYNHTFQWTDSLSWIRGAHSF
                     KFGADIRRDRYNQLGNQFARGSFIIENQATGYAFADFMLGYTRRDEDSAALAITQFRA
                     TSQAYYFTDTWKARSNLTIDWGLRYEYVPPWADRGNSLMNIWVPYSLRAPAVADPNLH
                     PVFVRIGRGDIYEGTVIRFNPAIKVVRDGRMGDRLVNDDKRDFAPRLGIAWSPTPKWT
                     MRTGAGFFYTQDTSNPVFDMGRNLGGRLRVEANTQTHDLTFDRPFGAGGGANPCNVPT
                     PPFVCISQPYVLGNKYDRTTPYVIQYEINLQRQLTNSTVLEAGYLGSQGHRLIRMFAF
                     NEAIPGETGSVVDRTPFQEFGRIQEIGNAGDSNYHSLSVKATRRMAAGLTYLAGYTFS
                     RSIDDGSGIRTLGSDPLFPQNSYCISCERGLSIFDTRHRFVTSLLYDLPFGKGRRFLN
                     HGIQSTLLGGWEVSSIFTISTGFPLTITTGIDRSNTGAGTDRPNATGQQVALDKGQRG
                     PAQWFNIGAFTLEPLGTWGNVGRNTLIGPGLVTWDFSTLKDFNFTESKYLQFRFEAFN
                     FPNHPNWSDPNTSLNSNRLDGTGHAISGTGNFGVISGTRTAMRQLQFSLKLVF"
     gene            complement(5168..5578)
                     /locus_tag="VFA54_00745"
     CDS             complement(5168..5578)
                     /locus_tag="VFA54_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49358.1"
                     /translation="MRSILIIGAALLLSKPAAPADDAFSRPEVTRAVATLNGVFSPRG
                     FFNITVRLYQLSGVEWAKFDLKKSQITLDFAPGVAVHPDEIQKVMVDAGYKPGPVRIQ
                     TLLPEDASKTGPGWVKIKHPSAKNPLLRWLQLNF"
     gene            complement(5581..6195)
                     /locus_tag="VFA54_00750"
     CDS             complement(5581..6195)
                     /locus_tag="VFA54_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49359.1"
                     /translation="MASYARYTLFKIDKPSSTFRIAPLAGAFIPTGSNSLTGPQGLLP
                     RTLQTGSGTLDSYFGVAMGYNNVRFGMAWDTTYRHNPVATTGVSPGDQFRTDAQFEVK
                     LYPIHMPEEGLPKLLVLSIESNFARDGKDHVAGALAPNSGGRVFKQDAILEISTLHWQ
                     VGAGAQFPVLQDLAGTGRIEQRSAFFLFFEYYLAAPNWRRSRGR"
     gene            complement(6694..8346)
                     /locus_tag="VFA54_00755"
     CDS             complement(6694..8346)
                     /locus_tag="VFA54_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS49360.1"
                     /translation="MKRGAHSVVILLSLFALFWRLDGTVLWRDEATTACWARSMVEHR
                     WLVPRVFDGRRLIAQAADGHDFNDHLLPVMQGWLQFYVAALGFLAAGASTVTARLPFA
                     VAGALSLWILYRAGRELLGDSLAALAAPLAGSASIYFLTAARQCRYYILVVLFTCCIL
                     REFGRYFREPERAQEWSFYARLGACGVLVYLANYVSFGGLWLSLAVFVWLVRDCALRR
                     GFVLLSAALAGPMAAEFFFVHAGFVAGTAAAHIGRWSDYAGAVREQGAEVFRMIPLLG
                     ILPAAWYVFFRRKQRGPAAQMALLAGCVIVISVAVTALGAKGDAIARYYFQILPAALL
                     LIGILAERIRALLGGVRGMLGSGVFFAFALVWPNLNIYHGWSGHVVERQFLKETTVNE
                     PIVRFLQTHVGRGETVAFYRNVQGMMAYFNLPWLQWVDLLDSNEPRNQRRRGILPAYV
                     FDDYPGVDWYVVWDNDGKMPRELTSEYRLVWEYTYTEHQSWWDRREPARVLTYRVYRR
                     MLYSASAPAVPTDRRPRAQQLRHALSEKSAAARGLGAQNVTE"
     gene            8468..8827
                     /locus_tag="VFA54_00760"
     CDS             8468..8827
                     /locus_tag="VFA54_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49361.1"
                     /translation="MRHRASYLIICGVLMAGLTGAAGRMQAQGTADRSPTIRQREVNQ
                     QKRIAEGIENGQLTPRETARIEKQESNLNQEIRRDRKTGGGLSPKERRQINRQQNRLS
                     REIYRQKHDSQTAPPAK"
     gene            complement(9177..9848)
                     /locus_tag="VFA54_00765"
     CDS             complement(9177..9848)
                     /locus_tag="VFA54_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZS49362.1"
                     /translation="MLRRNQIFRREFLSLASAALAARRAGRAQEKAALPRVLIIGDSI
                     SIGYTDFVRNLLQGKAEVVRIPVNGGPTTYGLENIQSWLGGGHWDVIHFNWGLHDIKL
                     DPAGRTRQVPIAKYEENLRLLVKTLKATGARLIWASTTPVPPVPWTKLDPPRSTSDVP
                     FYNAVAKKVMDENSIAIDDLYSFAFPRLETIQQPSNVHFTEEGYRELAQQVAASITAA
                     LDKIK"
     gene            complement(10008..10568)
                     /gene="efp"
                     /locus_tag="VFA54_00770"
     CDS             complement(10008..10568)
                     /gene="efp"
                     /locus_tag="VFA54_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZS49363.1"
                     /translation="MLQATQLRPGMVIKFNNELFSIFSVNHRTPGNLRGFVQVKMRNL
                     RSGSMIEHRFASEDRVEKAQLDEQEMEYLYDDGEYYYFMNTATYEQIHLTKDLLGEAV
                     QYLTPNLKVNVEFYEGKPLSVELPPTVDLKVLETEPALKGASVSNVTKPAKLETGLVV
                     QVPPFITEGEIIRVNTAEGTYQERAG"
     gene            complement(10858..14205)
                     /locus_tag="VFA54_00775"
     CDS             complement(10858..14205)
                     /locus_tag="VFA54_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49364.1"
                     /translation="MNPSRGLLFALSRLVLLFLLAIAVPFGSHAQLLQGTIDGNVADS
                     TQAAIVGARVVATNQETNFTRETVTNSAGGYDLPGLPPGTYTISVSSPGFQNYTQTGI
                     AVTPNNIRRVDVTLTVGQVNETVTVAASAAALQTDRAEIRSDVTGSTLANIPVPIGRN
                     YQMVVVTLPGVSPAQNANSFAANPSRSVNFSVNGTPTNINSTRIDGVSSGGIIDSTLT
                     YLPALEAIQEVNVVTNSFDAEQGLAGGAAISLQIKSGSNAIHGSLFGFHTDQHLKAYQ
                     WAADRTQPKPKYINNQYGATLGGPIKKDKLFYFASFEGTGYSENVTQVGQVPTPAMKA
                     GNLSGSPTPIYDPSTGNTNGTGRTAFPNNMIPANRIDPGVQNFLNLAGTWAPNQPGSG
                     ALGLSRNFVTSAGNYQWRRQYDDKLTWNPTNKLSTFFRFGLLDHNANTNGVFGQLGGL
                     PISRANTGAGFVQGHLFQGTISATYVATPNLVIDAAYGYFREDFNDVPPRSTENLGWT
                     VFQVPGLQTSDPRELAWPLILIDGFAQIGAPNNFEPHGYRDPVRDYMANLSWNKGSHN
                     LRVGTDINLQDINESQPQGANSYTAGPGGFQFSQGATQLNGGPAGNDYNAFASFLLGL
                     PANAGKLRLFPEEIQTRMKALSFYARDRWQVTPKLTVNYGVRWEYFPFPTRVGRGLEV
                     YDFQNNTMLLCGLGPNPIDCGITNGKGRPTPRAGIAYRLTDSTVIRAGYGMTNDPINF
                     ANFQRLNYPDLSQVVLNAPNAFSWATTLRQGFPAIVKPDLSSGILPVQGNLSLITFEN
                     NNLVRGYIQSWNFTVEKRIRGWIGSAGYVATRSVDQLAELDQNWSPIGGGAAGQQLNK
                     KFGRTASTFMMGTLGTAKYDSLQMRAEHRFSGGFHVSAGYTWAHGRGYTGESSGATPS
                     IGIPYYYGLNYGNLSRDIRHNLNASWIAEAPFGKGKPWLAEGSAARILGGWQLSGVFS
                     AYTGQPFTATASNTSLNAPGSSQRADCISTPQKIGSIFQWYSKSSFAVPAAGHIGTCG
                     YDSLTGPGVVNLDLGLDRKFQITERFELRFRAEAFNAANTPHHANPNSTQASVSNSSF
                     MQATDIRNTGRDGLDERTFRLGLKVSW"
     gene            14797..15429
                     /gene="rsmD"
                     /locus_tag="VFA54_00780"
     CDS             14797..15429
                     /gene="rsmD"
                     /locus_tag="VFA54_00780"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZS49365.1"
                     /translation="MRVIGGTFRSRHLKTVPRDSLRPTSDRLRETLFDVLGAEVEGKV
                     FVDAYAGSGAVGIEALSRGAARAIFIESNRKAAAVLRQNLRELGLETRSRVIERPASR
                     ALANIEADILFVDPPYANIHEYQACLSLAGQSAHGGGGADSFREDASRQKAAKASGTG
                     HRARVPGLVIAEHSSRIDLAPVYGKLQRTRVLKQGDSSLSFYRPRGSEQQ"
     gene            15617..16423
                     /locus_tag="VFA54_00785"
     CDS             15617..16423
                     /locus_tag="VFA54_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HZS49366.1"
                     /translation="MRTLAPGAVGADVSLLQLQLQKLGLYSGPVDGSFGPATCDAVRN
                     FQKNLGLAVDGVAGPRTVLALGLTGGRQPLAIPGVTFAVVLRMFPFAPAANIEANLPP
                     VLSALVKPGLTDKSMILMALSTIRAETEAFEPVSETESACNTSPGGRPFDLYENRPDL
                     GNVAPGDGARYRGRGFVQLTGRINYQQHGAAIGLGDRLTLEPELANDPEIAARLLASF
                     LKKREARIRGALGSGELAKARQFVNGGSNGLGRFVEAYSIGEKLIPDELA"
     gene            complement(16514..>17752)
                     /locus_tag="VFA54_00790"
     CDS             complement(16514..>17752)
                     /locus_tag="VFA54_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49367.1"
                     /translation="QADYMNFAPRLGIAWSPTPQWTVRAGAGIFYVQDTGNPRFDMSR
                     NIQGRITSTANIQTHNLTFENPFTGGSANVCGVPVPPFVCVTAPQGLANDSHRRTPYT
                     EQYELNFQRQLAANTVLEFGYLGTQGHRLERLISYNLPAPAPTGSVTARSPAPEFGNI
                     QFLAGVVNSNYHSFSAKLTRRMSAGLTFLGGYTWAKSIDDGSGIRVLGSDPLKPQDGT
                     CVSCERGLSIFDTRHRLVTSVLYDLPVGKGRKYLTHGLAGSLIGGWEFSNIWTVSTGF
                     PLSVLDGKDQSNTGHGYDKPNAVPGQSINLESSQRSTARWFNTSAVALQPLGTYGNLG
                     RNVVTGPGIFTMDFSTLKNFAFAERRYVQFRFEAFNVLNHPNFGDPNTSLAAGAFGSI
                     NSTRSGIDMRELQFSLKVVF"
BASE COUNT         3704 a         5283 c         5288 g         3477 t
ORIGIN      
        1 tgaccggagc atatgcgaac acccggccgc tctggcgcca tacgtccaaa tcgccctcgg
       61 aagcaacgct ctggatggcc cagaccaccg ccagcggcat ggcgccctgg tatcactggc
      121 tgggcggagc gcccgaggac ctgcgctggc gcgaaaccgg ccgcagattt tatcagtggc
      181 acgcccggca cgaaaagcac ttcgtcaacc ggcgctccat cgccaacctg ggcgtagttt
      241 tctccgaacg gaccaatgct ttctatcacc cgccgggagg tgacgatccg acggagtttc
      301 tccagggcct gtattacgcg ctgctggaag gccgtttctt tttcgacttc gttcatgaag
      361 acgatctcgt tcccgaaact ctcaagaaat ataaggcgct gctgcttgcg aacgtcgcag
      421 tgctgagcga tgaacagtgc cggcaattgc gcgattacgt ccaaagcggc ggctcgctgt
      481 tggccacctt tgagacgggg cggtatgacg atcgggggca agcgcgcagc ggactaggcg
      541 tcgaggagat ctttgagatg cggccgaagg gccgcgtgca agggccaaac ggcaatgcat
      601 tttacgcccg tatcgagcag tcgcacgaaa tcttgaacgg cttcgaaaac acgaagctgc
      661 tgccgggcgc agaataccgg cagcctgtgg aggcggccgg cggggcagtg ctgaccgtcg
      721 tgccgccttt cccggcattc ccacccgaga tggtgtacgc caatacgcct cacaccgatg
      781 agccggccat cgtgattcga gaaaaggcag cgagccggct gatctacttg tcgggggatg
      841 cggaccggag ttgctggcgg tcgggcaatc cggacctgag cctgctcctg ggcaattgcg
      901 tgcgctggct gcttcggggc tctccggtgg aagttgccgg agaaggaatg gccgagctgt
      961 ttgcgtggga gaccgagccg gggcttgcgg tacacgtcat caattacaac aatccgaata
     1021 tgatgcgcgg ctggatgcgg cggaactatc cgctcggccc gcagcgggtg cggatggagt
     1081 tgcccgccgg cttccatgtc tctcgcgttc aggctctgcg ggcggaaatc aacctgcctt
     1141 accgccagag cggccgccgg ctcgagttca cgatccccaa ggtcgaagac tacgaggtgg
     1201 cggcgctttc agcgtgacga cagcgctctt tttgtagaaa cacgcctcgc tattccgact
     1261 tctgagaatc ttagggcctc gctagccgct gtccgccgct ctcctcgaag ccggccaatc
     1321 cgaacggggc cgcaagcgca aagcccggcg caagcggaaa tccggatcgc ccgttgcaat
     1381 ccgcccagag ccggaacatg ccgcaacggc tggcatccgt gattatccct gaccgaacat
     1441 tggccgccgg cgcccccatt cacagtgttc ggccggcgaa agccttgcaa gaacgcgcga
     1501 gcattcgaaa ccgcggctta gccacttgtc agaaagcgct cgccatcgcc atggcggccc
     1561 gtgcggggca tctgcaaggt actggtacga ccctcttttg tcaattattt gtctctggcg
     1621 tgtaaaaaag atgttgacat aagcgccatg gcttggcaga atgttgccaa ctttcttggc
     1681 atcaaaggag ggtttatggg ttaccccttc tggtgtccct ggcgcgtgtt gtcgctgagt
     1741 ctgattttgg tttgggccac gaccgcccgg gcacagacac aaggacaaat tacaggagta
     1801 gtaacggatc cgagcggcgc gctggtatcc ggcgccaccg tcaccgtcgt caatccgcaa
     1861 accggtttca cccgccaggt acgaacgaat acagcgggca attacaactt cccggctctg
     1921 ctgccgggcc tctacgacgt aaaagtcgag atgcagggtt tccagaccga aatccgttcg
     1981 ggcgtcgagt tgcaggttca gcaagtggcg cgcattgact ttgcgctgcg cgtgggcgcc
     2041 gtggcggaaa cggttgaagt caccggaggg gctcccctgc tcaataccga gaacgccacg
     2101 gtggggactg tgattgagaa ccagcgcatc gtcgagttgc cgctcaacgg ccggaatttt
     2161 ctccagttgg tcgcgttgag ccccaacgtc aacgccagtt tcgcttcggc gggccagtcc
     2221 ggctcccggc aaggaggtga ccgcgccaac cagcagcttt cgatctccgg aaaccgccgg
     2281 gagtggaact atttcactct ggacggcgtc gacaacacgg atgttaattt caatacttat
     2341 acgttcctgc cgtctatcga tgcgttgcaa gagttcaagg tgcagaccgg catctattca
     2401 gccgagtttg gccgcgaaac cgggcaagtt aatgtctcaa ccaaaggcgg caccaaccag
     2461 taccacggag cactgttcga gttccttcgc aacagttccc tggatgcccg cccgtttgcc
     2521 ttcaccagcg cagtacccac caaagcccca ttccgctgga atcaatacgg cttcacgctg
     2581 ggcggcccgg tgcagatacc aaagctgttt aacggcaagg accggctttt cttcatgtct
     2641 aatttcgaag gcttccgctt gcgggagcag cgccagggca tctacagcgt tccctcggca
     2701 gccatgcgca acggcgactt ctccgaaatt cttcctacgc cgatcaatga tccgctgacg
     2761 aatcagcctt tccccaacaa caagataccc agcgcccgcc tgcatcccat cgcgctgaag
     2821 cttcttgagt tttatccccc gcccaatgtg cccggggccg gattgcagcg caactatctc
     2881 gctttgcaga acaaccggac cgacaaggat cagttcacgc agcgcatcga tttcgtcgag
     2941 agcgcgaagt caagctggtt tggccgctac agttggggcg atgaacaagc gatcgccccg
     3001 gctctgaaac tgaacggcac gaacctgcaa acgcacgtga atcagtggat gatcgacaat
     3061 acgcgagtcc tgcggccgaa catcgtcaat gaatttcggt tcggttacag ccatttcttc
     3121 aacagcttcg gccgcgagct cgctttccag cgcgatgtag taaaagagtt aggccttacc
     3181 gctgtgggct tgaacgaccc gcccccggtg gcgtggggca tccccgctat cagcatcacc
     3241 ggcttcagcg gattcggaga tgattctgaa ggcccctacg tcaactacaa ccacacgttc
     3301 cagtggacgg acagcctttc ctggatccgc ggagcccact ctttcaagtt cggtgcggat
     3361 atccggcggg accgctacaa ccagctcggc aatcagttcg cccgcggctc tttcattatt
     3421 gagaaccagg ccacaggtta cgcctttgcg gatttcatgc tgggctacac ccggcgcgac
     3481 gaggattcgg ccgcgctggc catcacccag ttccgggcca ccagccaggc ctactacttc
     3541 accgacacgt ggaaagcgcg ttccaatctg acgatcgatt ggggtctgcg gtatgagtac
     3601 gtcccaccgt gggcggaccg cggcaatagc ctgatgaaca tctgggtgcc ctatagcctg
     3661 cgggcgcccg ccgtcgccga tccgaacctg catccggtct tcgtgcgaat cggccgcggt
     3721 gatatttatg aaggcacagt gatccgtttc aaccccgcaa tcaaagtcgt gcgtgacggc
     3781 cggatgggcg accgcctggt aaacgacgac aagcgcgatt tcgcgccgcg cctcggcatc
     3841 gcgtggagcc cgactcccaa gtggacgatg cgtaccggcg ccggcttctt ttatacccag
     3901 gacaccagca acccggtctt cgacatggga cgcaacctgg gcgggcgcct gcgcgtggag
     3961 gccaatacgc agacgcatga cctgacattt gaccgtccct ttggagccgg cggcggggcc
     4021 aacccctgca atgtgcctac gccgccgttt gtgtgtatct ctcagccata cgtcctcggc
     4081 aacaagtatg atcgcaccac gccctacgtc atccagtatg agatcaacct tcagcgccag
     4141 ctcaccaact cgacggtgct cgaggcagga taccttgggt cccaagggca ccggctgatc
     4201 cgtatgttcg ccttcaacga ggccatcccc ggggagacgg gatccgtcgt ggaccgcacg
     4261 ccattccagg aatttggccg cattcaagag atcgggaatg cgggagattc caattaccat
     4321 tcgctctcgg tcaaggctac gcgccgcatg gccgccgggc ttacgtacct ggcgggctac
     4381 accttttccc gctcgatcga cgacgggagc ggaatccgca ccttgggaag cgatccgttg
     4441 tttccccaga acagctattg catcagttgc gagcgcggat tatcgatttt cgatacgcgc
     4501 caccggtttg tgacttccct gctgtacgac ctgcccttcg gcaagggccg gcggtttttg
     4561 aaccacggaa tccagagcac tctgctcggc ggctgggagg tcagcagcat cttcacgatc
     4621 tcgacgggtt tcccgctcac catcaccacc gggatagacc gttcgaacac tggcgccggc
     4681 actgatcgcc cgaacgccac tgggcagcag gtggccttgg ataaggggca gcgggggccc
     4741 gctcagtggt tcaacatcgg agccttcacg ttggagcctc tcggcacttg gggcaacgtc
     4801 gggcgcaaca ctctgatcgg accgggtctg gttacctggg acttttcgac tctcaaggac
     4861 ttcaatttca ccgagagcaa atatctccag ttccggtttg aagcgttcaa ttttcccaat
     4921 catccgaact ggagcgaccc gaacacgagc ctcaatagca accggctcga tggcacgggc
     4981 catgcgatct ccggcacagg caacttcgga gtgatcagcg gcacccggac ggctatgcgc
     5041 cagttgcaat tcagcctgaa gctggttttc tgaccagcgt tgcatcggcg ggtcttcggt
     5101 ctccgttacg cgcgcccgat gcgaaaaatg aaacgccgcc ggcgtcaaag cggccagcga
     5161 gtctacatca gaaattaagc tgaagccacc gcagaagtgg attcttcgca ctcggatgct
     5221 tgatctttac ccatccgggg cctgtcttcg atgcgtcctc gggcagaagc gtttgtatcc
     5281 ggacaggccc cggtttgtaa ccggcatcta ccattacttt ctggatctca tcggggtgga
     5341 cggctactcc tggggcaaag tcgagggtga tttgggattt ctttagatca aatttggccc
     5401 actcgacgcc ggaaagttga taaaggcgca cggtaatatt gaagaacccc cggggagaga
     5461 aaacgccgtt cagagtagcg acggcgcgag tgacttcagg ccgggaaaac gcgtcatccg
     5521 ctggcgctgc cggcttggag agcagcaggg ccgccccgat aatcagaatg gagcgcacga
     5581 tcatcgtccc ctcgaacgcc gccagttggg cgcggccaga taatattcga agaacagaaa
     5641 gaatgcgctt cgctgctcga tacgtcccgt tccagccaga tcctgaagca ctgggaattg
     5701 agcgccggcc ccgacctgcc agtgcagtgt ggagatctcc agaatcgcgt cctgcttgaa
     5761 gacgcggccc cccgagttcg gagccaatgc tcccgcgaca tgatccttgc cgtctcgagc
     5821 gaagttcgac tcgatcgaaa gcacaagcag cttcggcaag ccctcttccg gcatgtggat
     5881 cgggtacaac tttacctcga attgcgcgtc ggtccggaac tggtcgccag gactgactcc
     5941 ggttgtggcc actggattgt ggcggtacgt ggtatcccag gccatgccga aacgaacatt
     6001 gttgtagccc atcgcaacac cgaaataaga atccagcgtg cccgatcccg tctgtagcgt
     6061 ccgcgggagc agaccctggg gcccggtgag cgagttgctg ccggtcggaa tgaaggcgcc
     6121 ggccagagga gcgatccgga acgtggagct cggcttgtcg attttaaaaa gggtgtatcg
     6181 cgcgtacgat gccatgtcgc cgaacccgcc agaactcaat tcggcccggc cctgagacgt
     6241 cgcttgagtt aaagatccaa acccctggtt gagattcaaa aacaatgccc aacgagaagt
     6301 cagcccatag cccatgttca ccgggcattg aatgcccgag aattcatggg ttgcaaacgt
     6361 aggctgggca ttaaaacgga tcaaaagttg atccctggcg accggcagcg ccgtaacaaa
     6421 tgtcgcggct gaaggcaatg ccgggcgagc gaagctggtc agcagtagaa atacggcgag
     6481 tacacgattc agtggctctt ctccttaagt ggggagtagt gactgagagc ccggcttgca
     6541 tgcccggaat ggaaatacca agcctcctga ctacgccaac aacaacctat aagcgcgcgg
     6601 cttcccgccc tgagggaaac caaacgcgac gccggcgcgg ccagaggact ctctcaaacg
     6661 gcctattgta atcgaatctc aatccgactt tcatcattct gtaacattct gtgcgccaag
     6721 cccccgagcg gcggcggatt tttcggagag cgcatggcgc aactgttgcg cacgcggccg
     6781 ccggtcagtc ggaacggccg gcgcagaggc ggaatagagc atgcggcggt agactctgta
     6841 ggtcagcacc cgggccggct cgcggcgatc ccaccagctt tggtgctcgg tgtaggtgta
     6901 ctcccaaacc aggcggtact ccgaagtcag ttccctgggc atcttaccgt cgttgtccca
     6961 aacaacatac caatcgacgc cggggtaatc gtcgaaaacg taggccggca ggatgccgcg
     7021 gcggcgctgg ttgcgcggtt cgttgctatc gagcaggtct acccattgca gccacggcag
     7081 attgaagtag gccatcatcc cctgcacatt ccggtagaag gcgactgtct cgccccggcc
     7141 tacatgggtt tgcagaaagc ggacgatcgg ctcgttgacg gttgtctctt tcaagaattg
     7201 gcgctccacg acatgcccgc tccagccgtg atagatattc aggttgggcc acaccagggc
     7261 aaaggcgaaa aaaactccgc tgcccagcat tcctcgcacg cctcccagca gggctcgaat
     7321 cctttccgcc agaataccga tcagaagcaa agcggccggc agaatctgaa aataatagcg
     7381 cgcgatggcg tcgcccttcg ctccgagcgc cgtcaccgcc actgagatca caatcacgca
     7441 gcccgccagc aaagccattt gcgccgccgg gccgcgctgc tttctcctga aaaacacata
     7501 ccacgcagcg ggcaggatgc ccaacagggg aatcatccgg aaaacctcgg cgccctgctc
     7561 gcgaacggct ccggcgtagt cgctccaacg gcctatgtga gcggcggccg tgcctgccac
     7621 gaaccctgcg tgaacaaaga aaaactcggc tgccatcgga cctgccaggg cggcggaaag
     7681 cagaacgaag ccccgccgca aagcgcaatc gcggacgagc cagacaaaaa ccgccagcga
     7741 cagccacagc ccgccaaaac tgacgtagtt ggccagatac accaggacgc cgcaagcgcc
     7801 aaggcgagca taaaaactcc actcctgcgc ccgttccggc tcgcgaaaat agcggccgaa
     7861 ctctctcaaa atgcagcaag taaaaagtac tactaagata tagtaacggc actggcgggc
     7921 ggcggtcaga aaatagatcg acgccgatcc ggccagaggc gccgccagcg ccgccagcga
     7981 atcgccgagc agctcgcggc cggcgcggta caaaatccaa agcgagaggg cgcccgcgac
     8041 cgcaaacggc aaccgcgcag tcacagtgct tgcgcccgcc gcgaggaaac cgagcgcggc
     8101 tacgtaaaat tgcagccagc cttgcattac cggcaataga tggtcgttga agtcatggcc
     8161 atcggcggcc tgcgcgatca gccgccggcc gtcgaagacc cgcggaacga gccaccggtg
     8221 ctcgaccatg gagcgcgccc agcaggcggt ggtcgcctcg tcgcgccaca gcaccgtgcc
     8281 gtcaagccgc cagaacaggg cgaacagaga tagtagtatt actacactgt gcgcgccgcg
     8341 cttcattaga aaaaattcat gctctcccgc aatcgatacg ctattttcgc acgatctcgg
     8401 cgcaactttt cgcagccttt cgagtttgta ctcaacgagc ggtacaggaa aagcagcgga
     8461 ggaaaaaatg cggcatcgag cgagttatct cattatttgt ggagtcctga tggcggggct
     8521 gaccggcgct gccggccgga tgcaggcgca gggcacggcg gaccgttccc caaccatccg
     8581 gcagcgggag gtgaatcagc agaagcgaat cgccgaaggc atcgagaacg gccaactcac
     8641 gccccgggag acggcgcgca ttgaaaagca ggaatccaat ctgaatcagg aaatccggcg
     8701 cgaccgcaaa acgggaggcg gcctctcgcc gaaagagcgc cgccagatca atcggcagca
     8761 gaacaggctt agccgggaaa tctacaggca gaagcacgac agccagacag cgccgccagc
     8821 caaataagcg cccgccgcgg gcgctttggg cggactgccg gatctactcc acggccagtc
     8881 cgccccgcca gaatcgagag ccggggcgcg cgcatcaccc ctgaccacta caattgccgg
     8941 aatgcaagtg tgacgctcag gctcgcagga gatggcattt tctctttact gcctcgcggc
     9001 agcctgccgc gccagcgccc gccgctgttt ccaacggaag tccgcgcatt gcatcacaaa
     9061 gctttttgcg gcttttggac cttaggaacg atacttcgcc ggactcatgc cgggcggtgc
     9121 gagcgcaaat tgccggctgg ctgggaacgc gcccggaaga tatcggccct cgcggatcac
     9181 tttatcttgt ccagagcggc cgtgatggaa gccgccactt gctgagccag ctcccgatag
     9241 ccttcctcgg tgaagtgtac attcgatggc tgctggatgg tctcgagcct ggggaaggca
     9301 aaagagtaaa ggtcatcgat ggcgatgctg ttctcatcca taactttctt tgccacggcg
     9361 ttatagaatg gcacgtcgct cgtactgcgc ggcggatcga gttttgtcca gggcacaggc
     9421 ggcacaggcg ttgtgctcgc ccagatgagc ctggcgccgg tcgctttgag agtcttgacc
     9481 agcagccgga gattttcttc gtacttggca ataggcactt gccgcgttct gccggccggg
     9541 tccagcttga tatcgtgaag cccccagttg aaatggatga catcccagtg gccgccgccc
     9601 aaccaacttt gaatgttctc taggccataa gtggtgggtc cgccattgac gggtatccgg
     9661 accacttccg ccttgccctg gagcaagttg cgcacgaaat cggtatagcc gatcgagatc
     9721 gagtccccga tgatgagaac gcgcggcagt gcggccttct cctgcgccct tccggcccgc
     9781 ctggcagcca gagcggcgct ggcaagactg aggaactccc ggcgaaaaat ctggtttcgt
     9841 cttagcatcg gaatgtttgc tcaacaagat gccggctgct ggcaaatttc agcgcacaag
     9901 gccggatcgc ttcgcaaccc caaaggaccg caagcggccg gcccgggtcc caaagactcc
     9961 gcggcaacag cctggccgcg ctttcaacag cgagcctccc gcccctctca gccggcgcgc
    10021 tcctggtagg tgccttccgc ggtgttgacc cgaatgattt cgccctcggt gataaacggg
    10081 ggcacctgca cgaccagccc ggtctcgagc ttcgccggct tggttacatt cgaaacgctg
    10141 gcgcctttca gcgccggctc ggtttcgagc actttgagat caacggtcgg aggcaattcg
    10201 acgctgagcg gcttgccctc gtaaaattcc acgttgactt ttaaatttgg cgtcaggtat
    10261 tgcacggctt cgcccagaag atcctttgtg agatggatct gttcataggt ggcggtgttc
    10321 atgaagtagt aatactcgcc gtcatcgtac agatactcca tctcttgctc gtcgagctgc
    10381 gctttttcca cccggtcttc ggaggcgaag cgatgctcga tcatcgagcc gcttcgaagg
    10441 tttcgcattt tgacctggac aaagccgcgg agattacccg gagtccggtg gttcacgctg
    10501 aaaatggaaa atagctcgtt gttgaacttg atgaccattc ccggtcgtaa ttgggtagcc
    10561 tgcaacatag ggtttttagc ggatggccct caggataggg aaggaaacat tctgcccggg
    10621 gcccagtccc actattatat tgacacctaa gccgtatggt caaacagggg catgcgagcc
    10681 tgcggagagg gcagagagct tgctccgccc cggtttcgcc ggaagcaatg cggtggcacg
    10741 cttcttattt gcacgtggga ctgctgtgca aacgccactt tggagaaccc accggcaggt
    10801 gggcgccgct tcccgcgggt gatctccatc cactggcggc gttttcgccg ccagccgcta
    10861 ccagcttacc ttcaggccga ggcggaaagt ccgctcgtca agaccatccc ggcctgtgtt
    10921 ccggatatcc gtagcctgca tgaaagaact gttgctgaca gaggcctggg tggaattcgg
    10981 attcgcatga tgcggcgtgt tggcggcgtt gaacgcctca gcccggaatc gcaattcgaa
    11041 tcgctcggta atctgaaact tgcggtccag accgagatcg aggtttacta cacccggtcc
    11101 cgtgagactg tcgtagccgc aagtcccgat gtgcccggcg gccggaacgg caaaagacga
    11161 tttgctgtac cactgaaaga tgctgccgat cttctggggc gtggagatac aatccgcgcg
    11221 ctggctggac ccgggggcgt tcaaagaagt attggaggcg gttgcggtga atggctgccc
    11281 cgtgtaagcg ctgaaaacgc cgctgagctg ccatccgccc aggatccgcg ctgcggaacc
    11341 ttcggccagc cagggtttcc ctttgccgaa aggggcctcg gcgatccagc tggcattcag
    11401 gttgtgccgg atgtcgcggc tcaaattgcc gtagttcaag ccgtaatagt aagggatgcc
    11461 gatgctcggc gtggcgccgg agctctcccc tgtatagccc cggccgtgag cccacgtgta
    11521 accggcgctg acgtgaaagc ccccggagaa ccggtgctcg gcccgcattt gcagagagtc
    11581 gtatttcgcg gtgccgaggg tgcccatcat gaaagtggaa gcggtgcggc caaacttctt
    11641 gttgagctgc tggcctgccg cgcctccgcc gatgggcgac cagttctgat cgagctcggc
    11701 caactgatcc accgaacgcg tcgccacgta accggcggag ccgatccatc cccgtatccg
    11761 cttctccacg gtgaagttcc aggactgtat gtagccccgg acgagattgt tgttctcgaa
    11821 cgtgatcaga ctgagattgc cttgcaccgg caggatgccg ctgctcaggt ccggctttac
    11881 gatcgccgga aacccctgac gcaaagtggt cgcccaggag aaggcattgg gagcatttaa
    11941 caccacttgg ctgaggtccg gatagttcag acgctggaag ttggcgaaat tgatggggtc
    12001 attcgtcatg ccgtagccgg cgcgaataac ggtggaatcg gtaagacgat aggcgatgcc
    12061 ggcccgcggc gttgggcgcc cctttccatt cgtgataccg cagtcgattg gattggggcc
    12121 gaggccgcaa agcagcatgg tgttgttctg aaaatcgtaa acctccagac cgcgcccgac
    12181 gcgcgtgggg aaggggaagt attcccaccg gacgccgtaa ttcaccgtga gtttgggggt
    12241 cacctgccag cgatcgcggg cataaaagct taacgccttc atgcgagtct ggatctcctc
    12301 gggaaacaag cgaagcttgc ccgcattggc cggcaaccca agcagaaacg aagcgaacgc
    12361 gttgtagtcg ttgcccgccg gccctccatt gagttgggtg gcgccctggg aaaattgaaa
    12421 gccccccggg ccggccgtgt aggagttggc gccctgcggc tggctctcat tgatgtcttg
    12481 caaattgatg tcagttccga cccggagatt gtggctgcct ttgttccaac tgagattggc
    12541 catgtagtcg cggaccggat cacgataccc atgcggctca aaattattgg gggcgccaat
    12601 ctgggcaaag ccgtcgatga gaatcagcgg ccaagcgagt tctctggggt cggacgtctg
    12661 gaggcccgga acctggaaga ccgtccagcc gagattttcc gtcgatcgcg gcggcacgtc
    12721 gttgaaatct tcccggaagt aaccgtaagc ggcgtcgata accaaattcg gcgtcgccac
    12781 gtaagtcgcg gaaatggtgc cttggaacag gtggccctgc acaaatcccg cgcctgtgtt
    12841 ggcccggctg atcggaaggc cgccaagctg gccgaaaacg ccattcgtat tggcgttgtg
    12901 atcgagcaac ccgaatcgga aaaacgtgct caacttattc gtcgggttcc aggtaagttt
    12961 gtcgtcatac tggcgccgcc actggtaatt accggccgac gtgacaaaat tgcggctcag
    13021 cccgagggcg ccggaacccg gctgattggg cgcccaggtt cctgcgagat tcaggaaatt
    13081 ctgcactccc gggtcgatcc gattcgccgg aatcatatta ttcggaaacg ctgttcggcc
    13141 cgtgccgttg gtattcccag tagacgggtc gtagatcggc gtgggggaac cggaaagatt
    13201 acccgccttc atggccggag tggggacctg ccctacctga gttacgttct ccgaataacc
    13261 ggtaccctca aagcttgcga aatagaagag cttgtctttc ttgatgggtc ctcccagagt
    13321 cgcgccgtac tggttattga tgtatttcgg tttgggctgg gtgcgatcag cagcccactg
    13381 atacgccttc agatgctggt ccgtatggaa tccgaagagc gaaccatgga tcgcgttgga
    13441 tccgcttttg atctggaggc tgatcgcggc gccacccgca aggccctgct cggcatcaaa
    13501 ggaattggtg actacgttaa cctcttggat cgcttccagc gccggcaggt aggtcaatgt
    13561 actatcaatg atgccgccgc tcgaaacgcc atcgatccga gtgctgttga tattggtagg
    13621 agtaccgttg acggagaaat tgactgagcg gctgggattg gctgcaaagg agttggcatt
    13681 ctgggccggc gaaacgcccg gcagcgtcac caccaccatc tgatagttac gcccgatcgg
    13741 cactggtatg ttcgccaggg tgcttcctgt gacgtccgag cgaatctcgg cgcgatccgt
    13801 ttgtaaagcg gccgcagaag ccgcgacagt gacggtttca ttcacctggc cgacggtaag
    13861 tgtcacatcc acgcgccgga tgttattggg cgtgacggcg attcctgttt gcgtatagtt
    13921 ctgaaatccg ggagagctca ccgaaatcgt gtaggttccc ggcggcaacc cgggcagatc
    13981 gtagccgccc gccgagttgg ttacggtctc ccgggtgaaa ttggtttctt gatttgttgc
    14041 gacgactctg gcgccgacaa tcgcggcctg agtggaatcc gcgacattgc cgtcgatcgt
    14101 cccttgcagt aattgcgcat gagacccaaa gggtacggct atggctagaa ggaaaagtaa
    14161 aaccagccgg cttagcgcaa agagtaaccc tcgactcggg ttcattgaat cccccttctg
    14221 tccccgtatt tctaacgaga atgggtccaa tatacccgct tcttccagaa tgtcaacgac
    14281 gaaacccgag ctctccagca cagttgacat cagcgggcaa ggaaaagctt ctgtcacgtc
    14341 gaaacgccgc cccagttacg actttatatt tatataaaaa gattgtttaa ctccagatat
    14401 aataaataaa taatcatagc ttcatgtgat ataagctatc ttgcagcaac cggttggaat
    14461 gcgctcggcg gggagctcgc acccaacttc ggcgcgacct tccgcgaggt agcaagccgc
    14521 agaggttcgg cgggccgtcc aggcttgatt gacccgaaca tatcggagaa agacctcgat
    14581 actggcccgc tctaggaatt cccccctcga agcagggtgt acggggcatg ttttctggcc
    14641 atctggcaca atcaaactga gggcttgcca ggcgggaacg tgcgcgaccc cggaagaccc
    14701 cgacgggtcg tcggaagtgg ccaaatgaaa ggccggattg ctccgaggcc tgctggctca
    14761 atgacaagct cctcaccgcg gcttggagcc tgacctatgc gcgtgatcgg cggaacattt
    14821 cgttcgcggc atctgaagac cgtcccccgc gattcattac gtcccaccag cgatcggctg
    14881 cgcgaaacgc tcttcgatgt gctcggcgcc gaagtcgaag gaaaggtttt cgtcgatgcg
    14941 tatgccgggt caggagccgt cggcatcgaa gccttgagtc gcggcgcggc gcgagccata
    15001 tttattgaga gcaaccgcaa ggccgccgcc gtccttcgcc agaatttgcg ggagttgggt
    15061 ctcgaaaccc gcagccgggt tatcgagcgg cccgcgagca gagccctggc caatattgag
    15121 gccgatattc tatttgttga tccgccttac gccaacattc acgaatacca ggcctgcctc
    15181 agtctagccg gccagtcagc tcatggaggg ggcggcgccg attcattccg ggaggatgcc
    15241 agccgccaaa aagcagcgaa agctagcgga accggacatc gcgcgagagt tccgggcctg
    15301 gtgatcgccg agcattccag ccggatcgac ttggctcccg tctacgggaa actccagcgc
    15361 acgcgggttc tcaagcaagg agacagcagt ttgagcttct accggccccg aggctcagag
    15421 caacaatagc gagtccgccg gttcggcgaa gtgtcgctgc cggagcagcc agggtggtca
    15481 ccggctcgtc caattttttt gccacgcaga tgtaactact tggcttcatg gctggggaga
    15541 gggtaatttt tgccggatag gatgaagggg agggtgaaaa gtcttccttg actgctgaaa
    15601 ttgcatccgg ccaaagatgc gcacgctcgc ccctggcgcc gttggcgccg acgtttccct
    15661 tcttcaattg cagcttcaga agctcggatt atattcgggg cccgtggatg ggagttttgg
    15721 accggccacc tgcgatgcgg tacgaaactt ccagaaaaat ctgggactcg ccgtggacgg
    15781 cgtggccggt ccgcgaacgg ttttagcttt gggactgacg gggggccgcc agccgcttgc
    15841 cattcctggc gtgaccttcg ccgttgttct gcggatgttt cccttcgcgc cggcggcgaa
    15901 catagaagcc aacctgccac ccgtgctttc tgctctcgtt aagccgggat tgaccgataa
    15961 gagcatgatc ctgatggctc tttccacaat tcgcgccgaa acggaagcct tcgagccggt
    16021 cagcgaaacc gaatccgcct gcaacacctc gccaggtggg cggccctttg atctctacga
    16081 gaaccgcccg gatctaggca acgtcgcgcc gggagacggc gcgaggtacc gcggccgggg
    16141 ttttgtccag ttaacaggcc gaatcaacta ccagcagcat ggcgctgcaa tcgggctcgg
    16201 agacaggctc accctcgaac cggagttggc aaatgatccg gaaatcgcag ctcgcctgct
    16261 ggcgagcttc ttgaaaaagc gggaagcaag gatccgggga gcgctgggct cgggcgaact
    16321 cgccaaggcc cgccagttcg tgaacggcgg ctccaacggc ctcgggcgat tcgtagaggc
    16381 ctatagcatc ggcgagaagc tgattcccga tgaacttgca taatccagca ctcttgtccc
    16441 ctgaagtcag caacctcatt ccgccaggac tgaccggttt ggccagaagc cccggctccc
    16501 cgatcccgcg tttttaaaac accaccttga ggctgaactg caattcccgc atgtcgatgc
    16561 cgctgcgcgt actgttgatt gagccgaagg ccccggcggc cagactggtg ttcgggtcgc
    16621 caaaattggg gtgattcagc acattgaacg cctcaaatcg gaactgcacg tatcgccgct
    16681 cagcgaacgc gaagtttttc aaagtggaga aatccatcgt aaagattcca ggcccagtta
    16741 ccacattgcg gccgaggttg ccataggttc caagaggttg cagcgcgaca gccgatgtgt
    16801 tgaaccagcg cgccgtgctg cgctggctgg actcgaggtt gatgctttgc cccggcacgg
    16861 cattcggctt atcgtagcca tgaccggtgt tggactggtc tttgccgtcg agaacgctca
    16921 agggaaaacc ggttgagacg gtccagatat tgctgaactc ccagcccccg atgaggctcc
    16981 cggcgaggcc gtgcgtcagg tatttgcggc ccttgccgac gggcaggtcg tacagcacgg
    17041 acgtcaccag gcgatggcgg gtatcgaaga tcgacaaacc gcgctcgcag ctcacgcaag
    17101 tgccgtcctg gggcttcaga ggatcgctgc cgaggacacg gatgccgctg ccatcgtcga
    17161 tcgacttcgc ccatgtgtag ccgcccagga aagtgaggcc cgcggacatg cgccgcgtca
    17221 gcttggctga aaaggaatgg taattggaat tcacgacccc ggccaaaaac tgaatattgc
    17281 cgaactctgg cgccggcgag cgggccgtca cggacccggt gggggcaggg gctgggagat
    17341 tgtagctgat gagcctctcc aaccggtggc cttgggtccc caggtagccg aattcgagga
    17401 cggtattggc agccaattgc cgctggaagt tcaactcgta ttgctcagta taaggggtgc
    17461 gccggtggga atcgttggcg agcccctggg gcgcggtgac gcacacgaac ggaggcacgg
    17521 gcactccgca cacattggcg ctgccgccgg tgaaaggatt ttcgaaggta aggttgtggg
    17581 tttggatatt ggccgtgctg gtgatgcggc cctgaatgtt gcggctcata tcaaaccgcg
    17641 gattgcccgt atcctggacg tagaaaatgc cggcgccggc ccgtacagtc cattgggggg
    17701 tcgggctcca ggcgatgcca agccggggcg cgaaattcat ataatccgcc tg
//
LOCUS       DASREK010000012        10878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1982981, whole genome shotgun sequence.
ACCESSION   DASREK010000012 DASREK010000000
VERSION     DASREK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982981"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFA54_00795"
     CDS             complement(<1..196)
                     /locus_tag="VFA54_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49368.1"
                     /translation="MVKAIPITGGGPAGAAAGLAVTRAGAKAVIFEQSALPRHKLCGE
                     FFSPEIAVLFEQLGVMDGFRA"
     gene            complement(223..897)
                     /locus_tag="VFA54_00800"
     CDS             complement(223..897)
                     /locus_tag="VFA54_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS49369.1"
                     /translation="MTARPHFTELLDAPDAPPREVERSLGDLRRMNRWLGGRSVLLGI
                     VEREARRDGLTRFSLLDVGAGSGDQGAAVASRFPDARIVLCDRNCNHLPDARSGMLRL
                     AAEAGRLPFSDGAFDFVCASLVLHHFRDPDVVILLRLFGRVARRAVLINDLERHWFPL
                     AFIHVARPIFARSVLTRHDAPASVRQAFRPQELKNLALQAGFQNVEVRRHLPWFRLSL
                     VARNHA"
     gene            complement(899..2149)
                     /locus_tag="VFA54_00805"
     CDS             complement(899..2149)
                     /locus_tag="VFA54_00805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZS49370.1"
                     /translation="MRRRVVITGIGAVSPNGMGREAFWAATQCGVSGVKPIRLFDASS
                     LAVQVAGEVSGFDPDRYVSPKDRPHVSRVVPLARAAAEEALADAGIDPRRLQRDDVRR
                     ISVILGSGGGSQEFTEEQYRLYYTGQLKQVSVYTIPSSTMGTLASEVSMHFGFRGASH
                     VISTGCTSSSDALGYAFRQIRYGDAERILVGGADAPISYLIVRGFILMRILTPSWNHA
                     PARASRPFSRDRDGFVLGEGAWFFLLEPLEAARARGAHIYGEVAGYGSTCEAYHRVRL
                     EECGEEPARAMSLAIEDAGIPKDAIQYINLHGTATELNDRIETRAVRLCFDGHAEKLA
                     GSSLKSLIGHPQGACGSASVAATLLAMRDGVLPPTINLEVPDPECDLDYVPDIGRRAE
                     IECALINCIGFGSKNSALVLKRIE"
     gene            2400..3179
                     /locus_tag="VFA54_00810"
     CDS             2400..3179
                     /locus_tag="VFA54_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZS49371.1"
                     /translation="MHANHSQPHAPVNWQENLAIVLIETRNPLNIGAAARAMWNFGFS
                     RLALVSPYEVAFREARSAAGASLVLKEARVFSGLEEALAGCTLVAGTASAGRRVFQQP
                     RYPLADAAVLLREHLQREPAALLFGSEKFGLSNEHLSYCHWTLRIPTNSDCPSMNLGQ
                     AVAVCCYELARTTASPFPAITKGPGNRAAADQLQRLAARLSGVLDASGYINARTRRSN
                     LLKIRRLINRLNLSAADARVLEGMLRQIAWKLGCDNTQSDR"
     gene            complement(3127..3636)
                     /locus_tag="VFA54_00815"
     CDS             complement(3127..3636)
                     /locus_tag="VFA54_00815"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZS49372.1"
                     /translation="MNTTDLVHHTRYNAWATARVLESVRGLTPDQYTRGLGNSFGGIQ
                     GTLGHIYQADRIWFKRLAGEPTGSLAEFQPPAGRDAFEKEWLSVLDGYVSWAEKLSAS
                     DWDRVIQYRNTKGAALENPVWQIILHLINHDSYHRGQVTTMLRQLGANPVSTDLIAYY
                     RSLASRQSA"
     gene            complement(3766..4857)
                     /locus_tag="VFA54_00820"
     CDS             complement(3766..4857)
                     /locus_tag="VFA54_00820"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZS49373.1"
                     /translation="MPTTWMTTERTQTIEQYLGPGAEYLLGFNTPKISRDRLHLPGPD
                     AVDRIYAAADRNVRVLINLQRLLGHGRLAGTGYVSILPVDQGIEHSAGASFAKNSDYF
                     DPENIVKLAIEGGCNAVASTFGVLGAVARKYAHKIPFIVKINHNELLTYPNKFDQILF
                     GTVKQALDMGAAAVGATIYFGSEESGRQIVEVSEAFAMAHELGLATILWCYLRNSAFK
                     KDKDYHTSADLTGQANHLGATIEADIIKQKLPENNGGYKAMNSGGSGYGKFDERMYSE
                     LTTEHPIDLCRYQVANCYMGRAGLINSGGASGKNDFSEAVATAVINKRAGGMGLISGR
                     KAFQRPMKDGVALLHAIQDVYLDPGVTIP"
     gene            5324..5704
                     /gene="ndhC"
                     /locus_tag="VFA54_00825"
     CDS             5324..5704
                     /gene="ndhC"
                     /locus_tag="VFA54_00825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZS49374.1"
                     /translation="MLWPLVVYFVLVIALVAGMLIASHLLGQRHWEKATGSPYEGGIV
                     SEGSARVRLSARFYLVAMFFVVFDLEAVFLYAWAVAGRELGWAGYWEVVLFVGVLIAG
                     LVYLWRLGALDWAPELRRSLRRSR"
     gene            5720..6361
                     /locus_tag="VFA54_00830"
     CDS             5720..6361
                     /locus_tag="VFA54_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZS49375.1"
                     /translation="MQWSLTKPGAAGLIAKPDGEPFEQAVQRSVAFSRLQDLLAWGRK
                     NSIWPFNFGLSCCYVEMATSITSRYDVSRFGAEVIRGTPREADLMVIAGTVFIKMAPV
                     IQRLYEQMMEPRWVISMGSCANSGGMYDIYSVVQGVDKFLPVDVYVPGCPPRPDAFLE
                     GLVLLQKAVGTERRPLSWVVGSQETVRPAMPSMRDLMRPQRQKLKELREPSEI"
     gene            6358..8106
                     /gene="nuoC"
                     /locus_tag="VFA54_00835"
     CDS             6358..8106
                     /gene="nuoC"
                     /locus_tag="VFA54_00835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HZS49376.1"
                     /translation="MTVTLPGIEEIQEKLGGAAVTSQATRDGIPTFWVSKEYIRPLAR
                     YLKYEVERPYRMLYDITAIDERLRAHREGQPASDFTVVYHLFSYERNEYLRVKVPLEE
                     SRPRLESITDIWPSANWYEREVWDMFGVVFEGHPHLERILMPKSWQGHPLRKEHPARA
                     TEIGPFQLSEEKEDAEQEALRFRPEAWGMKRERDDADFIFLNIGPQHPGTHGVLRIVL
                     QLDGEVILDAVPEIGFHHRGAEKMGERQSWHTYIPYTDRVDYLGGVMNNLAYLTALEK
                     LAGIELPPRAKVIRIMLAELFRIISHLVWYGTFAQDLGQMSPVFYTFNDRERAFQIIE
                     AVCGARMHPSWFRIGGVAQDLPNGWEKLFRDFVKYLPPRLVEYDREVMRNRIFQARTK
                     GVGQITLDEAIDWGVTGPNLRACGFEWDFRKKQPYSGYEQFEFDIPTAQNGDCYDRAV
                     VRVEEMRQSLRIIDQCVKNMPEGPYKSDHPLATPPIKNFTMQDIETLITHFLNVSWGP
                     VIPPGEALGAIEATKGNNGYYLVSDGDTVSYRTRIRTPSFCHMQTLPLLCRGLMIPDL
                     LAILGSMDYVLADIDR"
     gene            8122..8586
                     /gene="nuoE"
                     /locus_tag="VFA54_00840"
     CDS             8122..8586
                     /gene="nuoE"
                     /locus_tag="VFA54_00840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZS49377.1"
                     /translation="MLSRQEIEEIEAEAARYPAREAVCIDALRIIQRRRGWVSDESIR
                     DLAELLGMSFADLDGVATFYNLIFRKPVGRHVIMICDSVSCWIMGYDRAREHLTQRLG
                     IGLGQTTPDSRFTLLPIVCLGCCDRAPAMMVDGDLHGDLNPEKIDAVLEQYK"
     gene            8583..9872
                     /gene="nuoF"
                     /locus_tag="VFA54_00845"
     CDS             8583..9872
                     /gene="nuoF"
                     /locus_tag="VFA54_00845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZS49378.1"
                     /translation="MSAANDKPLTGRIRADGSVVDLKAYEQAGGYQGLRKVLNGMTPK
                     QVTEQVKDSNLRGRGGAGFPTGLKWSFVPLGKDAPHPTYLVCNADEMEPGTFKDRLLL
                     ERDPHQLIEGMTIAGFAIEADVAYIFLRGEYVLAAERVGRAIAEAYQGGYLGKNILGS
                     DYSLELYLHLSAGRYICGEETGLLNALEGKRANPRAKPPFPPVCGLFGKPTVVNNVET
                     LCNVPHIVNHGAQWFQKLSRSKDGGTKLFGASGKVKRPGIWELPMGTTIREILEEHAG
                     GMRDGVRFRGLLPGGASTDFLTEQHLDTPMDFTEVAKAGSRLGTGTMIVLDDQTCPVG
                     MVWNLEKFFAQESCGWCTPCWSGLAWVERILRAMEDGQGQPGDLEKMEFQTRFWGNTF
                     CALAPGAAEPLQSALKYFRDDFERHIREKRCPWRWNG"
     gene            9865..>10878
                     /locus_tag="VFA54_00850"
     CDS             9865..>10878
                     /locus_tag="VFA54_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZS49379.1"
                     /translation="MARIFVDGKPYEMNPEQNLLHGCLSAGFNLPYFCWHPALGSVGA
                     CRQCAVKQFKDENDKRGRIVMACMTPAAEGTRISIDDPEAVTFRAAIIEGLMLNHPHD
                     CPVCDEGGECHLQDMTVMTGHDYREYRFEKRTFRNQYLGPMVNHEMNRCIQCYRCVRY
                     YRDYAGGRDLNSFSLHNTVFFGRHEDGVLENEFSGNLVEICPTGVFTDKTLKRHYTRK
                     WDLQFAPSICVHCGLGCNIDAGERYGTLRRVVNRYNYEVNGYFLCDRGRFGYEFVNDE
                     RRFRRPLLKGQPVSAEAALSRLRELVDRNKAIGIGSPRASLESNFALRKLVGEKRFFS
                     GSGD"
BASE COUNT         2126 a         3284 c         3423 g         2045 t
ORIGIN      
        1 gcgcccggaa accatccatg acgccgagct gctcgaacag gactgcgatc tccggggaaa
       61 aaaattcacc gcagagcttg tgccgcggga gcgccgactg ctcaaaaata accgccttgg
      121 cgccggcacg agtcacagcc agccctgccg ccgcgccggc ggggccgccg cccgtgatgg
      181 gaatggcctt aaccatggct gtctatctcc gggacgccga catcaagcat ggttgcgagc
      241 gaccagggac aagcgaaacc aaggcaggtg ccggcggact tccacatttt gaaaacccgc
      301 ctgaagggcg agatttttca gctcctgcgg ccggaaggcc tgccggacag aggctggcgc
      361 atcgtgacga gtcagaacgc tgcgcgcaaa aatcggcctc gccacatgga tgaaagcgag
      421 cgggaaccaa tggcgttcga ggtcattaat cagaaccgcc cggcgcgcca cgcggccgaa
      481 gagccgtagc aggatcacca catccggatc gcggaaatgg tggagcacta acgacgcaca
      541 cacaaagtcg aacgctccat cggaaaaagg caggcggccg gcttcggcgg cgaggcgaag
      601 cattcccgat cgagcatccg gcagatgatt gcaatttcga tcgcagagga cgatccgggc
      661 gtccggaaaa cgcgacgcca cggccgctcc ctggtcgccc gagccggcgc ccacatccag
      721 cagggaaaaa cgcgtaagcc cgtcgcggcg cgcctcgcgt tctacaatcc ccagcagaac
      781 gctgcgccct cccagccacc ggttcatgcg gcgcaagtcg cccaggctgc gctccacctc
      841 gcggggcgga gcatccgggg cgtcgagcag ctcggtgaag tgagggcgcg ccgtcatttc
      901 attcgatccg ctttagaaca agcgccgaat ttttgctgcc gaagccgatg caattgatga
      961 gagcgcactc gatttccgct cgccgtccga tgtccggcac ataatccaga tcgcattccg
     1021 ggtcgggcac ttcgaggttg atcgtcggcg gcaaaacgcc atcgcgcatg gcgaggagcg
     1081 tcgccgccac gctcgccgat ccgcaggcgc cctgcggatg gccgatcagg gacttgagcg
     1141 atgagccggc cagcttttcc gcatggccat caaaacacaa ccggacggcg cgggtctcga
     1201 tccggtcgtt cagttcggtt gcggtgccgt gcagattgat gtattggatg gcatcttttg
     1261 gaataccggc gtcctcgatc gccagcgaca tggcgcgcgc cggctcttcc ccgcactcct
     1321 ccagacggac gcgatggtag gcttcgcagg tggacccata gccggccact tcgccgtaaa
     1381 tatgagcgcc gcgggcgcgc gcggcctcca ggggctcgag caagaagaac caggcgccct
     1441 cccccagcac gaagccgtcc cggtctcgtg aaaaaggccg ggaagcccgg gcgggagcgt
     1501 gattccaact cggcgtcaaa atgcgcatca aaataaagcc gcggacgatc aagtaggaga
     1561 tcggcgcatc ggcgccgccg actaaaattc tttccgcatc gccatatcgg atctggcgaa
     1621 aggcgtagcc cagggcgtcg ctggaggagg tgcagcccgt ggagattacg tggctcgccc
     1681 ctcgaaagcc gaagtgcatg gaaacttcac tggccagagt ccccatggtc gacgagggga
     1741 tggtgtagac gctgacttgt ttcaactgtc cggtgtaata gaggcggtat tgctcctcgg
     1801 taaattcctg gcttccgccg ccggagccga ggatcaccga gatacggcgc acgtcgtccc
     1861 gctggagccg ccgcggatct ataccggcgt ccgccagggc ttcttccgca gccgcgcgag
     1921 ccaacggcac aacacgcgaa acatgcggcc ggtctttagg agacacatac cggtcggggt
     1981 cgaaccccga gacctcaccg gcgacttgca cggcgagcga ggaagcatcg aacaggcgga
     2041 tcggcttgac gccgctcacc ccgcactggg tggccgccca gaacgcctct cgccccatgc
     2101 cgttcgggct cacagcgcca atgcccgtaa tcaccactcg acgccgcatt tgattcgatg
     2161 ctagcaaacg gtttccgctg ccggatcagc cgaaatgtta aactccgcgc gagtgggccg
     2221 ccgaggcggc gcgccgcgag taaatgcaag cagcggaccg gcgtccgcgc gccgctgcgg
     2281 cttcggcgtc ccgaatcctt ccccgctgct ctgtccatgg agtttgaatt cccgtccggg
     2341 cggaaagagg cagaaaccgc tgtgcggcgg ccctgtgcca tcatgttagc cggcgcacga
     2401 tgcacgcgaa tcattcacag ccgcatgctc ccgtgaactg gcaggagaat ctggccattg
     2461 tattgatcga aacccgcaat cctctgaaca ttggcgccgc agcgcgagcc atgtggaatt
     2521 tcggcttttc ccgtttggcg ctcgtatcgc cgtatgaagt ggcttttcgg gaagctcggt
     2581 cggcagccgg cgcgtcttta gtcctgaagg aagccagggt gttctccggc ctcgaagagg
     2641 cgcttgccgg ctgtacgctt gtggcaggaa cggcgtccgc ggggaggcgc gttttccagc
     2701 agcctcgcta tccgctcgcg gacgcggcgg ttctcctgcg ggaacacctc cagcgcgagc
     2761 cggcggctct gctgttcggg tcggaaaaat tcggcttatc caatgagcat ctcagctact
     2821 gtcactggac cctgcggata ccaaccaact ccgattgccc ctccatgaat cttggccagg
     2881 ccgtcgccgt ctgctgttat gagctcgcgc gcacaaccgc ttcgccgttc cccgcaatca
     2941 cgaaaggccc cggaaaccgg gcggccgccg accagttgca gcggttagcg gcaagactca
     3001 gcggcgtcct ggatgcgagc gggtatatca acgcgcgcac acgccgctcg aacctgctga
     3061 agatccgccg tctgataaac cgtctcaatc tttccgccgc tgacgcccgc gtactggaag
     3121 gaatgctcag gcagattgcc tggaagctag gctgcgataa tacgcaatca gatcggtaga
     3181 gacggggttg gcgccaagtt ggcgaagcat cgtagtgact tgcccgcggt ggtaagaatc
     3241 gtgattgatc aggtgcagaa tgatctgcca caccggattt tcgagcgccg cccccttggt
     3301 gttgcgatat tggatgaccc ggtcccaatc tgaggcgcta agtttttcgg cccagctcac
     3361 ataaccgtcc agcacgctca accactcttt ctcgaaagcg tcccggccgg caggcggttg
     3421 aaattcggcc aggctgccgg tgggttcgcc ggcgagccgc ttgaaccaga tccggtcggc
     3481 ttgataaatg tggcccaggg tgccctggat cccgccgaag gagttgccga ggccgcgggt
     3541 gtactgatcg ggggtcaggc cccggacaga ctcgagcacc cgcgccgtag cccaggcgtt
     3601 ataccgagtg tgatgaacaa ggtcggtcgt attcattggg acgctccttc aaaaatctcc
     3661 gctcgcccgc gcaaacgccg cgccgcggcc tctcgaacaa gcctcaggtg cgcagccacc
     3721 ttgcaagccg ggccggaagt gtgatccggc gcggccaatc cggggctaag gaatcgtcac
     3781 gccaggatcc aggtaaacgt cctggatcgc gtgaagcagg gcgactccgt ccttcatggg
     3841 gcgttgaaac gcctttcgcc cggagatcaa ccccatgccg ccggcccgct tgttgatgac
     3901 tgcggtggca accgcttcac tgaaatcatt cttgccggag gccccgccgc tgttgatcaa
     3961 gccggcgcgg cccatatagc aattggctac ctgatagcgg caaagatcga tggggtgctc
     4021 ggtcgtcagc tcgctgtaca ttcgctcgtc gaatttgcca tagccgctcc cgccgctgtt
     4081 cattgctttg tatccgccgt tgttctcggg cagcttctgc ttgatgatat cggcctcgat
     4141 cgtcgcgccg aggtgattgg cctggccagt caggtccgcc gaggtgtgat aatccttgtc
     4201 cttcttaaaa gcgctgttac gcagatagca ccaaaggatg gttgccagtc ccaactcgtg
     4261 cgccatggcg aaagcctcgc tgacttcgac gatttgccgg ccgctttctt cagagccgaa
     4321 ataaatcgtc gcgcctacgg ccgcagcccc catatccaga gcttgtttga ccgtgccgaa
     4381 gaggatttgg tcgaacttgt tcggataagt gagcagctcg ttatggttga tttttacgat
     4441 aaagggaatt ttgtgcgcat acttgcgagc aactgctccc agcacaccga acgtggacgc
     4501 cacggcattg cagccgccct caatcgccag cttgacgata ttctccggat cgaagtaatc
     4561 ggagttcttg gcgaagctcg cacccgcgga gtgctcgatg ccctgatcga cgggcagaat
     4621 ggaaacatag ccggtgccgg ccaggcgccc gtggccgagg agccgttgca gattgatcag
     4681 cacgcggacg ttgcggtcgg cagccgcata gatccggtca acggcgtccg gccccggcag
     4741 atgcaggcgg tcccgggaaa ttttaggcgt gttaaagcca agcaggtatt ccgccccagg
     4801 accgagatac tgctcgatgg tttgggtcct ttctgtggtc atccatgtag tgggcatccc
     4861 tatctcctgt gggtcgatcg gccgtacaaa gaattacgga atccttgatt atagaaccga
     4921 tctgccatcg tggaacgccc cagcggctcc atcagaccat cccggcgccg gtgcggcatg
     4981 ggattcggag cgcccgcgag tctcgctgcg ccgcttctgg gcggatcgtc agatggcagg
     5041 gagacgatga ggcccgcgga cccgtgtttt cgtttgggcg gtttccctcc ggggtcggac
     5101 atttcacagc ggtatcgcac aagacccatg ttttgcgttg ggcggcccac tcctgtgcga
     5161 acaacatgct agactggtgg aactagacgc agaaagcgat tgaagaagtt cgcgcagtaa
     5221 gcgggtctgg gcgggtcgga cccataatct caagtcaccg agtccggcgt atcatccggc
     5281 tgggtatgca cctgaatcga gagatttgga aggcagcttc gtgatgcttt ggccgctggt
     5341 tgtctatttt gtgctggtaa tcgccctggt tgcgggaatg ttgatcgcct cgcatctgct
     5401 gggccagagg cattgggaaa aggcgacagg ttcgccttac gaaggcggca tcgtttcgga
     5461 aggatcggct cgcgtgcggc tttccgcccg attctatctg gtggccatgt tctttgtggt
     5521 tttcgatctg gaggcggttt ttctctacgc ctgggcggtg gcagggcggg aactcggctg
     5581 ggccggatac tgggaggtcg tgctttttgt gggagtactg atcgccgggc tggtttactt
     5641 gtggaggctg ggggcgctcg actgggctcc tgagctgcgc cgttcgctcc gccggtcgag
     5701 atagcggaag gcaatgggga tgcagtggtc attaacgaaa cccggcgccg ccgggctgat
     5761 cgccaagccg gacggcgagc cctttgagca ggcggtacag cgcagtgtgg cattttcccg
     5821 ccttcaggat ctgctggcgt ggggccgcaa aaactccatc tggcctttta atttcggcct
     5881 gtcctgctgc tacgtggaaa tggccacaag cataaccagc agatatgacg tttcccgatt
     5941 cggcgccgaa gtgatccggg gtacgccgcg cgaagccgac ttgatggtga tcgccggcac
     6001 ggtttttatc aagatggcgc ccgtgatcca gcggctctac gagcagatga tggaaccgcg
     6061 ctgggtgatt tccatgggct cctgcgccaa ctccggcggg atgtatgaca tctacagcgt
     6121 ggtgcagggc gtggacaagt tcctgccagt ggacgtctac gttcccggct gccccccgcg
     6181 tccggatgcc tttcttgaag gcctggtgct gctgcaaaag gcggtgggaa ccgaacggcg
     6241 cccgctgagc tgggtggtcg gatcccagga gacggtgcgg cccgccatgc cttcgatgcg
     6301 cgacctgatg cggccccagc gccagaagct caaggaattg cgggagccca gcgaaatatg
     6361 acggttacgc tccctgggat cgaagagatt caagaaaaat tgggcggcgc agccgtaacg
     6421 tcccaggcga cccgcgacgg aatcccgacc ttctgggtgt cgaaggagta catacgcccg
     6481 ctggcgcgct acctgaaata cgaggtggag cggccgtata ggatgctgta tgacatcacg
     6541 gccatcgacg agcgcctgcg cgcccaccgc gaaggccagc cggccagcga tttcaccgtc
     6601 gtctatcacc tcttttccta tgagcgcaac gagtatctgc gcgtcaaggt cccgctcgag
     6661 gaaagccggc cgaggctgga gagcattact gatatctggc cttcggccaa ctggtacgag
     6721 cgcgaagtct gggacatgtt cggcgtggtt ttcgaggggc atccgcacct cgagcgcatt
     6781 ttgatgccca aaagctggca ggggcatcct ctgcgcaagg aacatccggc gcgcgccacg
     6841 gaaatcgggc cgttccagct ctcggaagaa aaggaggatg ccgagcagga ggcgctgcgc
     6901 ttccgcccgg aagcctgggg aatgaagcgc gagcgggacg acgccgattt catttttctc
     6961 aacatcgggc cccagcaccc cgggacccac ggcgtgctgc gcatcgtgct ccagctcgac
     7021 ggcgaggtga tcctcgatgc cgtccccgag atcgggtttc accaccgggg cgccgagaag
     7081 atgggcgagc ggcagtcctg gcatacctac attccctata cggaccgcgt agattacctg
     7141 ggcggcgtca tgaacaatct cgcctatctc accgccttag agaaactcgc gggtatcgag
     7201 ctgccgccgc gcgcgaaagt catccggata atgctggcgg aactgttccg catcatcagc
     7261 cacctggtgt ggtatggaac tttcgcgcag gatcttgggc agatgtcgcc ggtcttttac
     7321 accttcaacg accgcgaacg cgcctttcag atcatcgagg cggtctgcgg cgcgcgaatg
     7381 catcctagtt ggttccggat cggcggtgtg gctcaggatc tgcccaacgg ctgggaaaag
     7441 ctgtttcgcg atttcgtgaa gtatctgccg ccgcggctgg tggaatacga ccgcgaggtg
     7501 atgcgcaatc gcatctttca agcgcggacg aaaggcgtgg ggcaaatcac cctggacgag
     7561 gccatcgatt ggggcgttac cggccccaac ctgcgcgcct gcggattcga gtgggatttc
     7621 cgtaaaaagc aaccctactc gggatacgag caattcgagt ttgacatccc gacggcgcag
     7681 aacggcgatt gttacgaccg cgccgtcgtt cgcgtggaag agatgcggca gagcctgcgc
     7741 atcatcgacc agtgcgtcaa gaacatgccc gaaggaccgt acaaatcgga ccacccgctg
     7801 gccacgccgc cgatcaagaa tttcaccatg caagacatcg aaaccctgat cacgcacttt
     7861 ctcaacgtga gctgggggcc ggtgattccg cccggcgaag ctctgggcgc catcgaggcg
     7921 acgaagggaa acaacggcta ttacctggtg agcgacggcg atacggtttc ctatcgcacg
     7981 cgcatacgca cgccctcgtt ctgccacatg cagacgctgc ctctgctgtg ccgggggctg
     8041 atgattccgg atctgctcgc catcctcggg agcatggact atgtgctggc ggatatcgac
     8101 cgctgaagaa ccggcaagat catgctgagt cgtcaagaaa tagaggaaat cgaagcggaa
     8161 gcggcccgct atccggctcg cgaagcggtg tgtattgacg cattgcgcat catccagcgg
     8221 cggcggggct gggtctccga tgagagcatc cgggacctcg cggaactgct cggcatgtct
     8281 ttcgccgatc tggacggtgt ggccacgttt tacaacctga tctttcgcaa gcccgtcggc
     8341 cgccacgtga tcatgatctg cgacagcgtg agctgctgga tcatgggcta cgaccgcgcg
     8401 cgcgagcatt tgacgcagcg gctgggcatc gggctgggcc agaccactcc cgacagccgg
     8461 ttcacgctgc tccccattgt ctgcctgggc tgctgcgacc gggcgccggc catgatggtg
     8521 gacggcgatc tgcacggcga tttgaacccc gagaaaatcg acgccgtgct cgaacaatat
     8581 aaatgagcgc cgccaacgac aagccgctca cgggcagaat ccgggccgac gggtccgtcg
     8641 ttgacctgaa agcgtacgag caggccggag gctatcaggg cttgcgcaag gttttgaacg
     8701 gcatgacgcc gaaacaggtg accgagcagg tgaaggattc gaacctgcgc ggccggggcg
     8761 gcgccggatt ccccaccggc ctgaaatggt cgttcgtgcc gctgggcaag gatgcgccgc
     8821 acccgactta tctggtctgc aacgccgatg agatggagcc gggaactttc aaggaccgcc
     8881 tcctgctcga gcgagatccg catcagctta tcgaggggat gaccatcgcc ggcttcgcca
     8941 ttgaagccga tgtcgcgtac atcttcttgc gcggcgaata cgtgctcgcg gccgaacggg
     9001 tgggccgcgc gatcgcggaa gcctatcagg gcggttattt ggggaaaaac attctgggct
     9061 ccgattacag cctggaactg tacttgcatc tgagcgctgg acgctacatc tgcggcgaag
     9121 agaccggcct gctgaacgcc cttgagggca agcgcgccaa cccgagagcc aagccgccgt
     9181 tccctccggt atgcgggtta ttcggcaagc cgaccgtggt gaataacgtg gagacgctgt
     9241 gcaacgtacc ccacatcgtc aaccacggcg cgcagtggtt tcagaagctc agccggagca
     9301 aagacggcgg cacgaagcta tttggcgcga gcggcaaggt caagcggcca ggaatctggg
     9361 aactgcctat gggcacgacg atccgcgaga ttctcgaaga gcacgcgggc ggcatgcggg
     9421 acggtgtgcg gttccggggc ttattgcccg gaggagcctc caccgatttt ctgaccgagc
     9481 agcacctcga tacgcccatg gactttaccg aagtggccaa agccggcagc cgcctgggaa
     9541 ccggaacgat gatcgtgctg gacgatcaga cctgcccggt cggcatggtc tggaacctcg
     9601 agaaattctt cgctcaagaa tcctgcggat ggtgcacgcc gtgctggagc ggcctggctt
     9661 gggtcgagcg cattctcagg gccatggaag acggccaggg ccagcccggc gatctggaga
     9721 agatggagtt tcagacccgg ttctggggca ataccttctg cgccctggcg cccggcgcgg
     9781 ccgagcccct gcaaagcgct ctcaaatatt tccgcgacga cttcgagcgg cacattcggg
     9841 agaagcgctg cccctggaga tggaatggct aggatcttcg tggacggcaa gccgtatgaa
     9901 atgaaccccg agcagaatct gctccacggc tgcctttccg caggcttcaa cctgccgtat
     9961 ttctgctggc acccggcgct gggttcggtg ggggcctgcc ggcagtgcgc cgtcaagcag
    10021 ttcaaagacg agaacgacaa gcggggccgc atcgtgatgg cttgcatgac gccggccgcg
    10081 gaaggtacgc gaatctcgat cgatgacccc gaggccgtga ccttccgcgc agccatcatc
    10141 gaaggcttga tgctgaatca tccccacgat tgcccagtgt gcgacgaagg cggagagtgc
    10201 cacttgcagg atatgacggt catgaccggg catgattacc gcgaataccg ctttgagaag
    10261 cgcacgtttc gcaatcagta tctggggccg atggtgaacc atgagatgaa ccgctgcatc
    10321 cagtgctacc ggtgcgtgcg ctactaccgt gactacgcgg gcgggcgcga cttgaactct
    10381 ttctcgctgc acaacaccgt cttcttcggc cggcacgaag acggggtgct cgaaaatgag
    10441 ttcagcggaa acctggtgga gatctgcccg acgggcgttt tcaccgacaa gacgctgaaa
    10501 cgccactaca cccggaaatg ggatttgcag ttcgcccctt ccatctgcgt ccactgcggc
    10561 ctgggatgca atatcgacgc cggcgagcgt tatggaacgc tgcgccgcgt agtcaataga
    10621 tataactacg aggtaaacgg atattttctc tgcgaccgcg gccgcttcgg gtacgagttc
    10681 gtcaacgacg agcgccgctt ccgccggccg ctgctgaagg gtcagcctgt ttccgcggag
    10741 gcggctctct cgcgcctgcg cgagctggtg gaccgcaaca aagcgatcgg aatcggctcg
    10801 ccgcgggcgt ctctcgagtc caatttcgcg ctgcggaagc tggtaggcga gaaacggttt
    10861 ttttccggat cgggcgac
//
LOCUS       DASREK010000013        14229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_995533, whole genome shotgun sequence.
ACCESSION   DASREK010000013 DASREK010000000
VERSION     DASREK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 14229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14229
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995533"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..3443)
                     /locus_tag="VFA54_00855"
     CDS             complement(189..3443)
                     /locus_tag="VFA54_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49380.1"
                     /translation="MGSKSHYAPGKDRRGGAAPPRCERLLFALAFACSILPGCLPPAG
                     AQTTSVIQGVVRDSQGLPIAGAEITLSGPLLAREARFTTDAAGSFRIPGLPAGSYNLR
                     AEKADFAAREYEGLTVTVNSVQAMDIELALGAVQEAVTVPADAPQMEPNSSSSGMTIQ
                     PQQIEQMPINGRNYLDLMQLVPGIAINRQANAGSDAAAPILGERSGNVIYLIDGMPNS
                     DVADGGPASPFDQDSILEFQVLTGGYKAEFGHGSGGVVNVVSKSGTNRWHGLASVFHR
                     NSFLDSSDVSGRAAPFLLRWDPSATLGGPILKDRAFVFASLERIRESRELNFSFPPGI
                     PDVLQAREESFDKHSQTFETRGLAKLDEQTGRHRLTEQLSLTNIHVTDFLPLSLATSL
                     PSARTNSDSRILAAGWHDTATLGGRSNPVLLNAYWQYFDEPFLQKPSHPEAGPAATAF
                     NMFSSLTTGRLSGDLGQVKFGAGFTPLRLDQQHISTGANLDKVLGAHEIKFGWDFQRA
                     RMDGAEATNLFNQLFATVSDFAQFGPVNSGVYTLTQVGGVTPQDNRIRLRNTYNGLFL
                     QDDWKAFPKLTLNLGVRWDYDSRFPNRANFSPRLGLAWSPASKTLLTAGWGIFYDHFR
                     LGLARDIPGFGGANIFRDQSVSFPRLFYGDPTTFPQNYGVCHSPVLTDAQIQSAGASC
                     PVAGLPFWGVDHLNAAVAPGHAPIPPNTVVTMDNVQALTGLDPQQFADAASAALGRPP
                     GFFFWGAFGNLTTNFLTPQTFAVPITVDPGFKTPYTADFHAGAQRAITQNLVIQAEYH
                     HRDIRNMLGVRTTNLAFAARLPGHTGELQPGTGPRPILSYGPWYRGTYDGISAGIRKR
                     MSRRFTAQVFYTWANAFDNALNSSLVSEVQTTSRGAGFLGANGPTDSFAGIPPIVTDP
                     ATGQTNAFGSFIASNGNPVPQAGKFYNGADLDRGPSDLALNHTLLLHGMIQLPWGLGA
                     SGIFRAQSGFHFTAASAPPADVDGDGILNGVDFLAGRNHFEAPAFVNLDMRFSKQFAI
                     KERVRVRAILEFFNLLNRANPAAVQQLENTPTPLGKPLQYLPGREGQIGLRFEF"
     gene            3778..7089
                     /locus_tag="VFA54_00860"
     CDS             3778..7089
                     /locus_tag="VFA54_00860"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS49381.1"
                     /translation="MAQVQWRTVFREIKLLPLLFLSAASLRIYVAPLAAQVGASILTG
                     RIIDPSQSAIPDAEVKVTNLASGSARTVRSNNDGIYRAPALVPGSYQIEVRAAGFEPA
                     VRRGVVLEVAQTVAADFVLQVGQQSQAMEVTGDIPAIETQSSSLGQLVNHKMIDNLPL
                     PNRSAASLVNLSPGVVMINSGEGAENYPVFSVAGGRARNQNFTLDGGNVTNAVGVTRP
                     QQQTSLPLDAMQEFRVISNNYSAEYGHSTGGVIAMSTRSGTNQFHGSVFEFARNNALD
                     ARNFFASVTPPLNLHQFGASLGGPLRKDKTHFFAAWEQTRETFGSAAIYTVPTLAERQ
                     GDFSRIPNAIYDPDTLAGGKKQPYPGNVIPRSQLDPVAVAALPYIPLPNRTPAASRAN
                     GDSNNFGANSHSSLRRDIVVGRLDHHLSGKDQLTTRYYVNDYGQVDGGAYGIPVSDPA
                     AGSTDGRVQSFMGSCLHTFSSTLVNSLQFSYDRRSFIQRRFGAGQGLAQSLGLLNVSR
                     AGFPTFNISGYAWLGAQGAANAAIARIQTPITDTQWTDAVSGFYGRHAVKIGLEYRRG
                     YNREINDPSSSGALAFTRQITDQPGMAGTGDGFASFLIGQASQAALQNTDSIPSHASY
                     WAAFAQDDHRITNNLTLNLGLRWEVELPRYVDHNRMNSFDRFAINPVSGTPGLVTFAG
                     VGGAPRTAFDANFANFGPRFGFAYSAPFFRNLVLRGGAGVFYGPMVSASVGPAAALGF
                     SDNLNLIAANPDLASALVLRNGFPLYTRPPITTPGFGAVPLGVAPTTAVTFFDRRRPT
                     PVSYQVNFDIQNEIRANLVIEAGYMGNVSHHLTANDLSINQVPPQRMGPGNAQLLRPF
                     PQFSNVSMINPPVGDSNYQAGFMKLERRFSHGFALLAHYTFSKFLDDAASSNELGDPG
                     SYMNAYNRKLDKGRSGSDIPHRAVITVLYQMPAFSGRPLASTLAGGWQAGLLGVLQSG
                     QPFTVYDSVNNTNAFPAGTPRPNLIGDPSAGPHTLARWFNTAAFQSAPAYTFGDSPRS
                     VLRGPSWKTVDLTLSRNLKLNERWSAELRGEFFNLLNHANFDIPGHTLGNADFGVISS
                     AEPARTVQVALRFVF"
     gene            7260..8138
                     /locus_tag="VFA54_00865"
     CDS             7260..8138
                     /locus_tag="VFA54_00865"
                     /inference="COORDINATES: protein motif:HMM:NF026155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49382.1"
                     /translation="MPRPPEQVWSSTLMNEGMTLSLEAEIEEIYADSEAKRDARIDQL
                     LTTFRQGLNRGEIRAAVFDGRWKTNVWVKKALSLCVRFGRLTQSAAGAPIDLDTLPRR
                     EFSLEDGVRVTDAGCFVRDGACVSAGCTLMPATVVQMGAYVGPQTVVGEGVSIGVCAQ
                     IGARVHLNPGVKIHGQLQPLEGLPAIVGDGASIGANCVIGAGVIVGPGAVIFPGTILL
                     WQTRLYDPLRKHRFLASEANPLMVPPGAIVVPGSRSLAKGAAGDALLSVQIGVIAGYI
                     TDHNLPAALLDRLLED"
     gene            complement(8124..8618)
                     /locus_tag="VFA54_00870"
     CDS             complement(8124..8618)
                     /locus_tag="VFA54_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49383.1"
                     /translation="MNPSAAYRIAASTPDLLVLQELPYAVAGWLALASGLFMAGVAAF
                     THWRFGSPRTTFWALLMGGGICALLGVWLMGLQYEFLFWRGQSAVEIRMSWFGRAVSD
                     ERLTYTVPPTADIVVTKRTTRQLVLRFHDGRTKTLGLSTDREGHEEAVQLINRFLRTG
                     SVLQ"
     gene            complement(8615..9352)
                     /locus_tag="VFA54_00875"
     CDS             complement(8615..9352)
                     /locus_tag="VFA54_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49384.1"
                     /translation="MRPPDPPLARIEIQALGRQHQYFAYLWACGDYPLAGEERDSYLG
                     TLAKLEKALDAAFGESQETLLQMWKLHHDGASKQLSKNGPAWAPVVGVGVWPHKPPPA
                     VWTREEFETFVPGEIPRPPISMVFRLKGERKQRTNVWPLLGGRGVVIQARIQGDPEEF
                     YKQCEQYFRTFITEPLHLAYPFFFPLFDSQTLPAIKPELADRLLGAVQYYLRESAEDG
                     GVLILSQEDLAPLFQELRWQVQPPPQP"
     gene            complement(9349..10257)
                     /locus_tag="VFA54_00880"
     CDS             complement(9349..10257)
                     /locus_tag="VFA54_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49385.1"
                     /translation="MPDVKSTPGPSRLTEAPGKEPVEPFRKAESLTRCELYWLPASLP
                     AKEQQRKWALQFLSELVKRLAADGRLQTELFVEAEAIYPDRQEQFIQTALDFNPVMGF
                     SRAPGAPLPSIPKTEDTEAIRRQEKPFELKPFLRGICYWFLRKNPQKQLELFWGWGGM
                     TMLLVKPDPAAQAPPLVVPAGVQKHPAYQSMAQQYDIPGMLSMAASAQSEFLKKSKKY
                     FGKGWEDRLEYRGLLYVLPRWCSQDFFNLPEEEVKHLFEVCDLFVTESPSDKGVLLAS
                     SKPIRPIIHEVVSSMREAGHVFPDRP"
     gene            complement(10266..11021)
                     /locus_tag="VFA54_00885"
     CDS             complement(10266..11021)
                     /locus_tag="VFA54_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49386.1"
                     /translation="MPIFEPAFDGQTKIGRRVDDPKTDKDLSGPGALNFGAMTTPAAL
                     AGCTGIDAKLVHGDRWQQIDGNFTEQVNANEMTTILGNQTRLVMGNRNLTTVGNVNST
                     TVSNLNQTVVGSTIRTYVGAAAYNYVSNHVATHASPQNRFEPGVYLHFVMSKLWAGNS
                     LHNIGMIYFQFYVLYTTITPTYLNLTPVLNFAATGINIHFEGFDNGSRALQNKLAAMQ
                     FQIDAMETEAELLELGTQMAIKSLSMAINSIAM"
     gene            complement(11076..12659)
                     /gene="vgrG"
                     /locus_tag="VFA54_00890"
     CDS             complement(11076..12659)
                     /gene="vgrG"
                     /locus_tag="VFA54_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HZS49387.1"
                     /translation="MAASFIKVLIDGQEISKFSEGAMLENVEVRQELNEHWWCDVEAR
                     QSGDQRFPIESCLGKDLSIAAVDEEGAEHILFDGFVLEAELEYEIYGSYTARLKGVDP
                     TYKLDFGRRFFRFDPTPLRDMVTMLCAPLNISSLVVCEEKSPPDPYIVWQESFWDFLK
                     RLADDHGCWIRYHPAEKSLQVVNTFQEENTVQWRAEDGLRSFKIRGKVARRTMSGANY
                     CRYQESKIYWQVRQVPEFMDGSGPMVEAVKEASEKTGNFGNGEVLERARAYTLDQYEA
                     LVKKECERTIGSAIVGSGESRNEELLAGNQIKIDGVLDARGTYGLTKVIHRWSPKGYR
                     NEFWCTPWTHYTNPEPPPVRKWNGVVQARVVENVDPQGFGRVKVQFYWDGDTPFWVRM
                     MTPHAGSDRGFYFIPEIGDEVVVAFQDGDPERPIVLGSIWNAVDRPPTEDFWGGEYGD
                     NDCKRIVTKSGHRIQLVDKKGKEAIAIATPQHIKIAMLENTNENGRPTMMLHCDGDIF
                     INAGGRIHLKSAFFSREVG"
     gene            complement(12706..>14229)
                     /locus_tag="VFA54_00895"
     CDS             complement(12706..>14229)
                     /locus_tag="VFA54_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS49388.1"
                     /translation="HSGAITPENSQAVNRSLLDLHSSLFSLTASRDREASFESADWGG
                     GHGIFTYYVVKGLEGEADQNADGIVTADELAEYVHRNVREATNARQNPTSERGSFDPN
                     MPLAYIPKTFARRAPAAQTSDFGALVLEANMDGVEVFVDGKSSGVVNKAAPLKLQGLR
                     PGPHTIQGVKMGYEPDGPREEMVYPGQETTVSIKIVYVRRRNKKASDLLDRGLEYYNK
                     GYAENYRKAAEFFEKALAEDPKFSQAAMYLGRSYNALYEDDKAQKAFRRALEIDPDSI
                     EARISFGGMLLDIGDFDEAIRQLNTVVQREPKQALGLSLLAQAYRMKDMYPQSIESAR
                     KAIRLLPENPEAHFFLADSLRLSGSYVEAEKEYREYLRLSDFQSKLAGKLNYYVLGFL
                     AGAGKKKRAAQQDIWKDLQSLAYFGLGDCERLLSHPDPAIENYTRALAYDKDDPKVHW
                     ALGLAYTKKAELTGNLDSLPQARQHFAAMLQINDQLAEAEQAKKYIARIDALLKSRQ"
BASE COUNT         2668 a         4500 c         4124 g         2937 t
ORIGIN      
        1 gacccgccgg cgccgcatgg aacgcgatca acagcgcaaa cacaagccac gcgatggccc
       61 gctgtgcggc ggtcgccttc cgatacccag ggtgcggcga ggataacaag caccattagt
      121 ctaaggctgg ccggcgatgc ctgtcaacgg aaaaacacac gcgcgcccga gcgctgcgct
      181 cgtttgcctc aaaactcgaa gcggaggccg atctgccctt cgcgtcccgg cagatattgc
      241 agcggcttgc cgagcggcgt gggcgtgttc tcgagctgct gcaccgcggc cggattcgcc
      301 cggttgagca ggttgaaaaa ctccaaaatc gcacgcacgc gcactctttc tttaatcgcg
      361 aattgtttag aaaagcgcat atccagattc acgaaagcgg gcgcctcaaa atgattgcgg
      421 ccggccagaa aatccacgcc gttcaaaatg ccgtctccat ccacatcggc cggcggcgcc
      481 gaagccgccg tgaaatgaaa gccgctctgc gcccgaaaga tcccgctggc gcccaagccc
      541 caggggagct gtatcatgcc gtgcagcagc agtgtgtgat tcagcgcgag atcggaaggc
      601 ccgcgatcga ggtccgcccc gttgtagaac ttccccgctt gcggcaccgg attgccgttg
      661 ctcgcgatga aagagccgaa tgcatttgtt tgcccggttg ccggatctgt aacgatcggc
      721 ggaatgccgg caaagctgtc ggtcggcccg ttggccccca gaaaccctgc cccgcggctg
      781 gtggtttgca cctcgctaac gagactcgaa ttcagggcgt tgtcgaaagc gttcgcccaa
      841 gtgtaaaaaa cttgggccgt gaagcggcgg ctcatgcgct tgcggattcc ggcactgatg
      901 ccgtcgtaag tgcctctgta ccacgggccg tagctcagaa tcggtctcgg gcctgtgccc
      961 ggctggagtt cgcccgtgtg ccccggaagt ctggctgcga aagcgagatt cgtcgtccgg
     1021 acgccgagca tattccgtat gtcccggtga tggtattccg cctgaatcac caggttctgc
     1081 gtgatcgcgc gctgcgcccc ggcatgaaaa tcggccgtat agggcgtctt gaatcccggg
     1141 tccacggtga tcggcaccgc gaaggtctgg ggcgtgagaa agttcgtcgt cagattgccg
     1201 aatgcgcccc agaagaaaaa tcccggcggc cgccccagcg ccgcgctcgc ggcgtcggca
     1261 aactgctgcg gatcaagccc cgtcagagcc tgcacgttgt ccatcgtgac caccgtgttc
     1321 ggtggaatcg gcgcgtggcc cggcgccacg gccgcattca agtggtccac tccccagaac
     1381 ggcaagcccg ccaccgggca agacgcccct gccgactgga tttgggcgtc cgtcaaaaca
     1441 ggcgagtggc acactccgta gttttgcgga aacgtcgtcg gatcgccgta aaacagccgt
     1501 gggaatgaaa ccgactggtc ccggaaaata ttggcgccgc caaagcccgg gatatcccgc
     1561 gccagaccca gccggaaatg atcgtagaaa attccccagc cggcggtgag cagcgttttc
     1621 gaagccggcg accaggcgag gcccaggcgc ggcgaaaagt tggcccgatt ggggaagcgc
     1681 gaatcataat cccagcgcac gcccagattc agcgtgagct tcgggaaagc cttccagtcg
     1741 tcctggagga aaagcccgtt ataggtgttt cgcagccgga ttcggttgtc ctgcggagtc
     1801 acgccgccca cctgggtcag tgtgtacacg cccgaattga ccggaccgaa ttgggcgaaa
     1861 tccgaaaccg tcgcgaatag ttgattgaag agattcgtcg cttccgcgcc gtccatccgc
     1921 gctcgttgaa aatcccatcc aaacttgatt tcatgcgctc ccagaacctt gtcgaggttc
     1981 gcgcctgtcg aaatgtgttg ttgatcgagg cgcaacggcg tgaaccctgc tccgaacttc
     2041 acttgcccca gatcgccgct gagcctgccc gtcgtcaagc tgctgaacat gttgaacgcc
     2101 gtagccgccg gcccggcctc cggatgcgag ggtttctgga gaaacggttc gtcaaagtat
     2161 tgccaatagg cgttcagcaa aactggattg ctccggccgc ccagagtggc cgtatcgtgc
     2221 cagccggctg caagaatccg gctgtcggag ttggtcctgg cggagggcag actggtggcc
     2281 agcgaaagcg gcagaaagtc ggtcacatgg atattggtga ggctcaactg ctcggtgagg
     2341 cgatgccggc ctgtctgctc gtcgagcttc gccaggcccc gcgtctcaaa agtctggcta
     2401 tgtttgtcaa aactctcctc gcgcgcctgg agaacgtccg ggatgccggg cgggaagctg
     2461 aagttcagct cgcggctttc ccggatccgc tccagggaag caaaaacgaa ggcccggtct
     2521 ttcaggatgg gtcctcccag ggtcgcgctc ggatcccagc gcagcagaaa tggcgctgcg
     2581 cggcccgaga cgtcggacga atcgaggaaa gaattgcgat ggaacaccga ggccagcccg
     2641 tgccaccgat tcgttccgct cttgctgacc acgtttacaa caccccctga tccgtgcccg
     2701 aattccgcct tgtaaccgcc cgtgagcact tgaaactcca agatcgagtc ctggtcgaag
     2761 ggagaagcgg ggccgccgtc ggcgacatcg ctgttgggca tgccgtcaat cagatagatt
     2821 acgttgccgc ttcgctctcc aaggatcggc gcggcggcgt cgcttcccgc gttcgcctgg
     2881 cggttgatgg cgatgcccgg aaccaactgc atcaaatcaa ggtagttgcg cccgttgata
     2941 ggcatctgct cgatctgctg cggctggatc gtcattccgg aggaagaaga gtttggttcc
     3001 atctgcggcg catcggcggg aacggtgacc gcctcctgta ccgctcccag cgcaagctca
     3061 atgtccatcg cctgtacgct gttgaccgtg accgtcagcc cttcgtattc ccgggcggcg
     3121 aaatcggctt tttcggcgcg caaattgtac gatcccgccg gcagcccagg aatccggaac
     3181 gagccggctg catccgtggt aaaccgcgcc tcccgggcga gcagggggcc gcttagagtg
     3241 atctcggccc ccgcgatcgg caagccctgc gagtcccgga caaccccttg aatcacagag
     3301 gtggtctgcg cgccggccgg cggcaggcat ccgggaagga tcgagcaggc aaacgccaac
     3361 gcgaaaagca ggcgctcgca gcgaggcggg gccgctccgc cccggcggtc tttgccaggc
     3421 gcataatgcg actttgatcc catttgtgtt cgggacgccg gtagcgcccg attctgctat
     3481 atcggtgatt cctgccggcg gccgtaggtc tggaagagcg ccaatgggcc tcgcagagct
     3541 ttttttcagc agcgccgctg gttgccggcc aatccgccgc gcgaagccgc cggcgccaac
     3601 cggcccaccc ggcaaaatgt tcttgtcttc ctgatggaaa tatactaatc tctatagaca
     3661 tgatagggat tgctctgcgt cccccatttc cgcgctgttg aagcacccgc tattgcggct
     3721 ctccaaagaa ctaactgcct ttttttcaat ctaatactca caaaaggaga atttggcatg
     3781 gcacaggttc agtggcgaac ggtattccgc gagatcaaac ttcttcccct ccttttcctg
     3841 agcgcggcct ctcttcggat ttatgttgcg cccttggcgg ctcaagtggg cgccagcatt
     3901 ttgaccggaa gaatcatcga cccatcgcaa tctgccatcc cggatgccga ggtcaaggtg
     3961 acgaatttgg cgtcaggttc ggctcggacg gtgcgctcca acaacgacgg catctaccgg
     4021 gcgccggcgc ttgtgccggg aagctatcag atcgaggtcc gcgcggcggg ctttgaaccc
     4081 gctgtccgca ggggagtcgt cctcgaggtg gcgcagacgg tcgctgccga cttcgttctg
     4141 caagtgggcc agcagagcca ggcgatggaa gtcacgggcg acattccggc gatcgaaacg
     4201 cagtcctcga gcctcggcca gctagtgaat cacaagatga tcgacaacct tccgctgccc
     4261 aaccgctctg ccgcgtcgct ggtcaacctt tctcccggcg tagtcatgat caacagcggt
     4321 gaaggcgcgg aaaattatcc ggtgttctcg gtggctggag ggcgggcgcg caaccagaat
     4381 ttcacgctgg acgggggtaa cgtgacgaat gcagtgggcg ttacccgccc gcagcagcag
     4441 actagcctgc cgctcgacgc catgcaggag tttcgcgtga tctcaaataa ctactccgcc
     4501 gagtatggcc actccactgg cggcgtcatt gcgatgtcca cgcgctcggg tacgaaccaa
     4561 tttcacggca gcgtgttcga gtttgcgcgc aacaatgcgt tggacgcgcg gaatttcttc
     4621 gcttctgtca cgccgcctct gaacctgcac cagttcggcg cctcgctcgg cggtccgctc
     4681 cggaaagaca aaacgcattt ctttgccgcc tgggaacaga cccgggaaac atttggcagc
     4741 gctgctatct acaccgtccc gaccttggcc gagcgccagg gcgacttctc tcggatcccc
     4801 aacgccatct acgatccgga taccctggcc ggcggcaaaa aacagccgta cccgggaaac
     4861 gtcattccgc ggagccagct agatccggtt gctgtggccg ccctgcctta cattcctctg
     4921 ccgaatcgca cgccggcagc tagccgggcc aacggcgact cgaacaattt cggcgccaac
     4981 agccattcct cgctgcggcg ggatatcgta gtagggcgcc tcgatcatca cctgagcggc
     5041 aaagatcagc tcaccacccg ctattacgtc aatgactacg gccaagtcga cggcggcgcg
     5101 tacggcattc ctgtctccga tccggcggcc ggctctactg acggccgggt gcaaagcttt
     5161 atgggcagtt gcctgcacac tttcagctcc accctggtca atagtctcca gttcagttac
     5221 gaccgccgga gctttatcca gcgccgcttc ggggcgggtc aggggctggc gcagtcgcta
     5281 ggtcttttga acgtgagccg cgccggtttt cccaccttca atatcagcgg gtatgcctgg
     5341 ctgggcgcgc aaggcgcagc gaacgccgcc attgcgcgca tccagacgcc cattacagat
     5401 acccagtgga ccgacgccgt ttccggattc tacggccggc acgccgtcaa aatcggcctt
     5461 gaataccggc gcgggtacaa ccgcgaaatc aacgacccat cgtcctccgg agctcttgcc
     5521 ttcacacgcc agattacaga tcagcccggc atggcgggca cgggcgacgg cttcgccagt
     5581 ttcctgatcg gtcaggccag ccaagccgcg ctccagaaca cggatagcat tccttcccat
     5641 gcttcttact gggcggcttt tgcgcaggac gatcatcgaa tcacgaacaa tctcaccctg
     5701 aatctcggcc tgcggtggga agtggaattg ccccggtacg tagatcacaa ccggatgaac
     5761 tcctttgacc gcttcgcgat caatcccgtt tccgggacgc cgggccttgt caccttcgcc
     5821 ggcgttggcg gcgcgccgcg aacggccttt gacgctaact ttgccaattt cggcccgcgc
     5881 ttcggatttg cctatagcgc gccgttcttc aggaatctgg tgttgcgcgg cggagccggc
     5941 gtcttttacg gacccatggt gagcgccagc gtcggtcccg cggccgccct gggcttttct
     6001 gacaatctga atctgatcgc ggcgaacccc gacttggcct cagctctcgt cttgagaaat
     6061 ggttttccgc tttatacgcg gccgcctatc actacccccg gattcggagc cgttcctctc
     6121 ggtgtggcgc ccacgacggc ggtcacattt ttcgaccgcc gccggcctac gcccgtctcg
     6181 taccaggtga acttcgatat tcagaatgag atccgcgcga atctcgtgat cgaggccggt
     6241 tacatgggca acgtgagtca tcacctgacg gcgaacgacc tgtctatcaa tcaggtcccg
     6301 ccccaacgga tgggccctgg caatgctcag cttttgcgcc cgtttccgca gttcagcaac
     6361 gtctcgatga tcaacccgcc ggtcggcgat tcgaattacc aggctggttt tatgaagctg
     6421 gaacggcgct tcagccatgg cttcgccctg ctggcgcatt ataccttctc gaaattcctc
     6481 gatgatgccg cttcctcaaa tgaactggga gatccgggaa gctacatgaa cgcctacaat
     6541 cgcaaactgg acaagggcag aagcggcagc gacatcccgc accgggccgt catcactgtg
     6601 ctctatcaga tgccggcctt ttccggccgc cccctggcaa gcacccttgc cggcgggtgg
     6661 caggctggcc ttctgggcgt gttgcagtcg ggccagcctt tcacggttta tgattcggtc
     6721 aacaacacga atgcctttcc ggccggaact ccacggccga atttgatcgg agatcctagc
     6781 gccggcccgc acacgctggc ccgttggttc aacacggcgg cgttccagag cgccccggcc
     6841 tatactttcg gcgattcccc gcgatcggtg ctccgcggcc cgtcttggaa aaccgtagat
     6901 ctgactcttt ccaggaacct aaagctcaac gagcgctgga gcgccgagct gcgcggggag
     6961 tttttcaatc tcctcaatca cgccaatttc gacatccccg gacacacgct cggcaatgcc
     7021 gatttcggag tgatctcctc ggcggagccg gcgcgcaccg tgcaagtggc gctccggttc
     7081 gtcttttgat tcagtctgtc tttccattgg atccggactg tttgctgatc gcgtgcaggc
     7141 aatactccaa cccgggccgc gcgcgcccgg cccggctgta ttgcggcggg gccgggaaga
     7201 aaggtaaagt gagcgaacgg acgcgtcgga tagcccggcg ggacggcgag tttctgccaa
     7261 tgccgcgtcc tccggagcag gtttggagtt caacgctgat gaacgaggga atgactctaa
     7321 gtctggaagc cgagatcgag gagatctacg ccgactccga ggcgaaacgc gacgcccgca
     7381 tcgaccaact cctgaccacg tttcgccagg ggctgaaccg cggcgaaatc cgcgccgcag
     7441 tgttcgatgg ccgctggaag accaacgttt gggtgaagaa agcgctctcc ctgtgcgtgc
     7501 gcttcgggcg cttgacgcag agcgccgcag gagcgcccat cgacctcgat acgcttcctc
     7561 gccgagagtt ctcgctcgag gatggagtcc gcgtcacgga tgccggctgt ttcgtgcgcg
     7621 acggcgcgtg cgtttcggcc ggctgcacct tgatgccggc tacggtggtc cagatgggag
     7681 cctatgttgg gccgcagacg gtcgtgggtg agggcgtgag catcggcgta tgcgcgcaga
     7741 tcggggcgcg ggttcacctc aaccccggag tcaagatcca tggccagctt cagcccctcg
     7801 aggggcttcc cgcgatcgtc ggcgacggcg ccagcatcgg cgcgaactgc gtgattggcg
     7861 cgggcgtgat tgtcggcccg ggcgctgtca tcttcccggg cacgattctc ctgtggcaga
     7921 cgcggctgta tgatccgctt cggaagcacc gctttctggc cagcgaggcc aacccgctca
     7981 tggttccccc cggcgccatt gtggtgcccg gcagccgctc gctggcgaaa ggagctgccg
     8041 gcgatgctct gttgagcgtc cagatcgggg tcatcgccgg atacatcacc gatcacaact
     8101 tgcccgcggc gctgctcgac cggctactgg aggactgatc ccgttcgcaa aaagcggttg
     8161 atcagttgaa ctgcctcctc gtggccttcc cgatccgtcg atagacccag cgtttttgtg
     8221 cgcccgtcat ggaaacgcag cacaagctgc cgcgtggtcc tcttggtgac cacgatatcc
     8281 gcggttggcg gaaccgtgta ggtcaaacgc tcgtcgctga ccgcccgccc gaaccaggac
     8341 attcggattt ccactgccga ctgcccgcgc cagaacaaaa attcgtattg cagccccatc
     8401 agccagaccc cgagcagcgc gcatatgcct cctcccatca aaagcgccca aaatgtagtc
     8461 cggggagaac caaaccgcca gtgcgtgaac gcggcaacgc cggccatgaa taggccgctc
     8521 gccagagcca gccatccggc tacggcgtac ggcagctcct gcaacacgag cagatccggt
     8581 gtggaagccg caatccgata tgctgccgac gggttcacgg ctgtggcggc ggctgtactt
     8641 gccagcgaag ttcctggaac aggggagcca gatcctcttg ggaaaggatc agcaccccgc
     8701 catcctccgc gctctcgcgc aggtaatact gcaccgcgcc caagaggcgg tccgccagtt
     8761 ccggcttgat cgccggcagc gtctgcgaat caaacagcgg aaagaagaag ggatatgcca
     8821 gatgcaaggg ctcggtgatg aaggtgcgaa aatactgttc gcattgcttg taaaattctt
     8881 cgggatcccc ttgaattctg gcctggatca cgacgccacg gccgcccagc agcggccaca
     8941 cgttcgtgcg ctgcttgcgc tcgcctttca gccggaacac catggaaatc gggggcctgg
     9001 ggatctcgcc cgggacgaat gtctcgaatt cctcgcgcgt ccagactgcc ggcggcggct
     9061 tgtgaggcca cacccccacc ccaaccacag gcgcccaggc cggaccgttc ttggaaagct
     9121 gcttgctggc gccgtcgtga tgcagtttcc acatctgcaa caaagtctcc tgcgattcgc
     9181 caaacgccgc atccagagcc ttctccaatt tggcgagcgt cccgagatac gagtcgcgct
     9241 cttcgccggc cagaggataa tcgccgcaag cccacaggta cgcgaaatac tgatgctgcc
     9301 gcccgagggc ttggatctct atccgagcta agggagggtc cggcggtctc atggcctgtc
     9361 ggggaacacg tggcctgcct cgcgcatgct gctgaccacc tcgtgaatga tcggacgaat
     9421 gggcttgctc gaagccagaa ggacgccttt atcagacgga ctctccgtca caaaaagatc
     9481 gcagacctcg aacagatgct ttacctcttc ctcgggcaga ttgaagaaat cctggctgca
     9541 ccaccgcggc agtacgtaca gaagcccccg gtattcgaga cggtcctccc atcctttccc
     9601 aaagtatttt ttactcttct tgagaaattc gctttgcgcg gaggccgcca tgctgagcat
     9661 gcccgggatg tcgtattgct gagccataga ttggtaagcc ggatgtttct ggactccggc
     9721 gggcaccacc aaaggcggcg cctgcgcggc cggatccggt ttcaccagca acatcgtcat
     9781 cccgccccac ccccaaaata attccaactg cttttgcggg ttcttgcgga gaaaccaata
     9841 acagatcccg cgcagaaaag gcttcagctc aaacggtttt tcttgacgcc ggatggcctc
     9901 ggtatcctct gtcttcggaa tggaaggcag tggagcgccg ggggcccggc tgaaccccat
     9961 gaccgggttg aaatccagcg ccgtctggat gaattgctcc tgacgatccg ggtaaatcgc
    10021 ttcggcctcg acgaacagtt ctgtttgcag ccgcccgtca gcagccagcc gcttcaccag
    10081 ttctgacagg aactggagcg cccatttgcg ctgctgctct tttgccggca gagaggctgg
    10141 cagccaatac agctcgcagc gggtcaggct ttcggccttg cgaaaaggct ctaccggctc
    10201 ttttccggga gcctccgtaa gacgagaagg gccgggggta gattttacgt ccggcatgat
    10261 tgcattcaca tcgcaatgga gttgattgcc atggaaagag acttgatggc catctgcgtc
    10321 cccagctcca aaagctccgc ttcggtctcc attgcatcga tctgaaattg catagcagcg
    10381 agtttgttct ggagcgcgcg gctgccgttg tcgaaacctt cgaaatgtat attgattccg
    10441 gttgcagcaa agttcagcac tggggtgaga ttcagatagg tgggtgtgat cgtcgtataa
    10501 agtacataga actgaaaata gatcatcccg atgttgtgca gactgttgcc ggcccagagc
    10561 ttactcatca cgaaatgaag gtaaaccccc ggctcaaacc tgttttgcgg ggaagcatgg
    10621 gtcgcgacgt ggttggacac gtagttgtag gcggcagcgc ccacgtacgt ccggatcgtc
    10681 gagccgacaa ccgtttgatt gagattgctg accgtcgtgc tgttcacgtt tccaacggtt
    10741 gtcaggtttc gattgcccat caccaatcgc gtctggtttc cgaggatcgt ggtcatctca
    10801 ttggcgttaa cctgctctgt gaagttcccg tcgatctgct gccagcggtc gccgtgcacc
    10861 agtttggcgt caatgcccgt gcatccggcg agggccgccg gtgtggtcat ggctccgaag
    10921 tttaacgcgc cgggtccgga aagatctttg tccgttttgg gatcgtcgac cctgcgcccg
    10981 attttcgttt gtccgtcgaa agcaggttcg aaaataggca taagatgttc ttcctccgaa
    11041 actggttttt tctacgcggc aattttccgc gcagcctagc cgacttcccg cgaaaaaaac
    11101 gcgctcttca ggtggatgcg gccgccggcg ttgataaaaa tatccccgtc acaatgcagc
    11161 atcatcgtcg gccgcccgtt ttcgttcgtg ttttccagca tggcgatctt gatgtgttgc
    11221 ggcgtcgcga tggcaatggc ttctttgccc ttcttatcca cgagctggat gcggtggccg
    11281 ctcttggtga caatccgctt gcaatcattg tcgccatatt ccccgcccca gaagtcctcc
    11341 gtggggggcc tgtcgacggc attccagatc gatcccagga cgatgggccg ctccggatct
    11401 ccgtcttgaa acgcgacgac cacttcgtcg ccgatctccg ggatgaagta gaacccgcgg
    11461 tccgagcccg cgtgaggcgt catcatccgc acccagaatg gcgtatctcc gtcccagtag
    11521 aactgaacct tcaccctgcc gaaaccctgc ggatcgacat tctccaccac cctcgcctgg
    11581 acgactccat tccacttcct caccggcggc ggttccgggt tcgtgtagtg ggtccagggt
    11641 gtgcaccaga actcgttcct gtaccctttc ggactccagc ggtggatcac cttagtcagc
    11701 ccataggtgc cccgcgcatc cagcacgcca tcgatcttga tctggtttcc cgccagcagt
    11761 tcctcgttgc ggctctcgcc ggagccgaca atggcgctcc cgatcgtccg ctcacattct
    11821 tttttcacca gagcctcata ttgatccagc gtgtaggccc gcgcccgctc caatacttcc
    11881 ccatttccaa agtttcccgt tttttccgag gcctccttca cggcctccac catcggcccg
    11941 ctcccgtcca tgaactcggg cacttgccgg acctgccagt agatcttgct ttcctggtac
    12001 cggcaatagt tcgcgccgct catcgtcctt ctggcgacct ttccccggat tttgaagctg
    12061 cgcaggccgt cttccgcccg ccactggacc gtgttctcct cctgaaacgt gtttaccact
    12121 tgtagcgact tttccgcggg atggtaccgg atccagcatc cgtgatcgtc ggccagccgc
    12181 ttcaaaaagt cccaaaagct ctcctgccag acgatgtagg gatccggcgg gctcttttct
    12241 tcacacacca ccagagagct gatattcagc ggagcacaga gcatggtcac catgtcgcgc
    12301 aatggcgtgg ggtcgaaacg aaagaacctt cgcccgaaat ccaatttgta cgtcgggtcc
    12361 acgcccttca agcgggcggt ataactgccg taaatttcat attcgagttc ggcctccagc
    12421 acgaagccgt cgaacaaaat gtgttcggcg ccctcttcat ccaccgcggc gatgctcagg
    12481 tctttgccca ggcagctctc gatcggaaat cgttggtctc cgctttgccg cgcctcaacg
    12541 tcgcaccacc aatgctcgtt cagttcctga cggacctcta cgttttcgag catcgcgcct
    12601 tcgctgaact tgctgatttc ctggccgtcg atcaggacct tgatgaagct ggctgccata
    12661 agagatcttc tccctccgcc atccttgctt tcgcaacgcc gcgggctact gccgggactt
    12721 cagcagggca tcaattctag caatatattt cttggcttgc tcagcctcgg cgagctgatc
    12781 gttgatctgg agcatcgccg caaaatgctg gcgcgcctgc ggcagagaat cgaggtttcc
    12841 cgtcaattcg gcctttttcg tgtacgccag gcccagggcc cagtgaacct tcggatcatc
    12901 cttgtcgtat gccagcgccc gggtgtaatt ttcgatcgcc gggtccggat gcgacaacag
    12961 ccgctcgcaa tcccccaggc cgaaatacgc cagactctgc aagtccttcc agatgtcttg
    13021 ctgcgccgcg cgcttcttct tgcccgcgcc cgccagaaag ccgaggacgt agtagttgag
    13081 tttgccggcc agcttgcttt gaaagtcgct cagccgcaga tattcccgat actctttctc
    13141 cgcttccaca tacgatccgc tcaggcgcag gctgtcggcg agaaaaaaat gggcctcggg
    13201 gttttccggc agcagccgga tggccttgcg cgccgattcg atcgattgcg ggtacatgtc
    13261 tttcatgcgg taagcctgcg ccagcaggct caggccgaga gcctgcttcg gttcgcgctg
    13321 caccaccgta ttgagctgcc ggatcgcttc atcgaaatcg ccgatatcca gcagcatccc
    13381 gccgaagctg atccgcgcct cgatggaatc ggggtcgatc tcgagcgccc tccggaaggc
    13441 cttctgcgcc ttgtcgtcct cgtaaagcgc gttgtaggac cgccccagat acatcgccgc
    13501 ctggctgaac ttgggatcct ccgccagcgc cttctcgaaa aattcagcgg ccttcctgta
    13561 gttttccgcg tagcccttgt tgtaatattc cagcccgcga tcgagcagat cagacgcttt
    13621 cttgttccgc cggcgcacgt aaacaatctt gatgctgacc gtagtttcct ggcccggata
    13681 taccatctcc tcgcgcggcc cgtccggctc gtagcccatc ttgacgccct ggatggtgtg
    13741 cggcccgggc cgcaggcctt gcaatttcag gggcgcggct ttgttcacca cgccggacga
    13801 tttgccgtcc acgaaaactt ccaccccgtc catattcgcc tccagcacca gggcgccgaa
    13861 gtcggacgtc tgggctgccg gcgcgcggcg ggcgaaagtt ttcggaatgt aggccagcgg
    13921 catattcggg tcgaagctgc ctcgttcgga agtcgggttc tggcgggcgt tcgtcgcctc
    13981 ccgcacgttg cggtgaacgt actcggccag ttcgtcagcc gttacaatgc catcggcgtt
    14041 ctggtcggct tctccctcca gtcctttcac cacgtagtag gtgaagatgc cgtggccgcc
    14101 gccccagtcg gcgctttcaa aagaagcttc ccggtcgcgg ctggccgtca gtgaaaacag
    14161 cgagctgtgc agatcgagaa ggctccggtt gacggcctgg ctgttttcgg gcgtgatcgc
    14221 tccgctgtg
//
LOCUS       DASREK010000014        11563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2315510, whole genome shotgun sequence.
ACCESSION   DASREK010000014 DASREK010000000
VERSION     DASREK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315510"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFA54_00900"
     CDS             <1..459
                     /locus_tag="VFA54_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49389.1"
                     /translation="NPEDIPALIEKLHADGYDMVTGSRFLKRNGYPIQPLRRLGGLLF
                     SVILYPILGKRITDPTSGFAAVNRRALEVFSRSFPLEYPEIEALVVLQRKALRFCEVP
                     VRMFPRRAGRSTIGSLQAIYYMFRVLLGVMVNVLKYERRFHTGARRPPGA"
     gene            463..855
                     /locus_tag="VFA54_00905"
     CDS             463..855
                     /locus_tag="VFA54_00905"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HZS49390.1"
                     /translation="MERIRIVVAVLTALLIVQIVRSVRREHIRVEYSMTWLGATLLLL
                     ILCLSDKAMGLLSGLVGVRDASLVLIILAGVLFVFTFFRYSVVVSSLKDHNIQLAQKV
                     GMLEWEIERLNKLLHAVQNPEPESRDSK"
     gene            852..1961
                     /locus_tag="VFA54_00910"
     CDS             852..1961
                     /locus_tag="VFA54_00910"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZS49391.1"
                     /translation="MRIVYDGTPLLMRSAGVKNYHYALLTRLLPAIHPHTIELFPFLP
                     GLGRLRNEHSNYSRPATILRLAAVLSDNYLRLPLGSWAARRADLFHLTHHLLHPPERG
                     ALRLTSLVHDPTCVLMPEFHTPSNVRYFRRFVQEVMPRLSGIIVPSEAVKRDLIEKLH
                     ACRERITVIPHGVEERFFQTPRQPARLPQGVPERYILSLGAMEPRKNLTALLEAYQLL
                     PEDLRQAYPLVIAGASGWKNERLQRNLQDRPSVCAIGYIDADLLPMLYARASVFVFPS
                     LYEGFGMPLLEAMAAGAPVVASNVSAIPEVVGEAGLLVDPRSPSEIARAIERVLDDRD
                     LAENLAARGALRARQFTWQKTAAATRSFFECVAGS"
     gene            1994..3037
                     /locus_tag="VFA54_00915"
     CDS             1994..3037
                     /locus_tag="VFA54_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HZS49392.1"
                     /translation="MPLVSAQELRSLSQRIFVAAGVPPDVAQRVAESLVESNLLGHDS
                     HGVIRVPSYLTMLGDGRIDPKAQPRVARETESTALVDGGWQFGQVTARYAMELAVSKA
                     ARTGVAAVQLYQSGHIGRLGEYAHQAAEQGMVGLVCTNNHTSGQILAPFGGTARRLSP
                     SPIAIAAPGGQDFPIVLDMTTSTTAEGKIRVRQARGQRLPEGWIIDAAGRPSTDPADF
                     YGPPPGSLLPLGGELGYKGFGLAFMIDILSGAFGGSGCSQANPPQLRGNGVFLQAIDP
                     GAFGAKESFKREVAALAEYVKSSPVAPGFDRIRIPGETEHEARQQRMQHGIEIEEDTW
                     SKLRAAAAELGLK"
     gene            3034..3744
                     /locus_tag="VFA54_00920"
     CDS             3034..3744
                     /locus_tag="VFA54_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1698 domain-containing protein"
                     /protein_id="HZS49393.1"
                     /translation="MMDRAELARAVAAIRWAHRIDLGQGIVTPGIWDTPQILKRLHLP
                     ADLTGQTVLDIGCWDGFYSFEAERRGALRVLATDSFTWQRKSWGSKDGFNLARQALRS
                     RVEDLEIDVLDLAPETVGVFDVVLFLGVLYHMKHPLMALERVASVTGRLLIVETVIDL
                     LCIPGKALKFYPGDELDGDESNWFGPTPSAVEAMLRAAGFRRVERKWPAGSRLLLWRA
                     LSLVGSRATKRAVFHAYK"
     gene            complement(3917..4645)
                     /locus_tag="VFA54_00925"
     CDS             complement(3917..4645)
                     /locus_tag="VFA54_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49394.1"
                     /translation="MSPHELLFALACMGMYVVLLSTMLRGSAARYPFLFAYLIVNLLS
                     TVVQGSFKYYFGPRSREYVLAYWVSDFVGTFLILMIIIHLIRAAMERHKYRNAVYFGL
                     LLGAVTTGVLSVVFTHSHSWDKMFRPLMTKVGRDYYFAAVLLNAILWIMLTKSWNPNK
                     QLYLITSGLGLQLSGAAIAHALRVTHHFMSLGAYLLAPTYLLNLYVWYIALAKLPSEA
                     PVVAGTEMPPAAAPQPKPTSFHYH"
     gene            complement(4701..5048)
                     /locus_tag="VFA54_00930"
     CDS             complement(4701..5048)
                     /locus_tag="VFA54_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49395.1"
                     /translation="MKKIVVHSVICVTICTLFVLAAYAQQQVGVLGPQTAAVALLTGA
                     TAPPETLDVALQNGPWDPPPEDDIPALRASLQNGPWDPPPEDDIPVLKVRVKNGPWDP
                     PPEDDIPFARFGM"
     gene            complement(5270..5911)
                     /locus_tag="VFA54_00935"
     CDS             complement(5270..5911)
                     /locus_tag="VFA54_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS49396.1"
                     /translation="MTSIVLADDHTIVREGIAAILSAEPDFKIVGQAADGEQAFEMIK
                     SLQPDVAIVDLQMPKLYGIEVIRRTRKWNTRIRMGVLSINRDQKTITDALNTGANAYL
                     LKDGPARHLVDAIRIILEGGLYISPLLQPQAFLASTRRPAAADPLESLSSREYQVFGF
                     LVEGLRAKEIAARLDISPKTVDTYRSNIMRKLHIYDVPGLVKYALQRKLTSLE"
     gene            complement(5926..6990)
                     /locus_tag="VFA54_00940"
     CDS             complement(5926..6990)
                     /locus_tag="VFA54_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZS49397.1"
                     /translation="MSALAFAPAPATRHRPEAAQKPALVDQDQPLLIRFDSSGEIQWM
                     NDAAREALRGRSSVLAVLASEKPLPPLSRHRLGAEWLWLGISPGDWGARFRQRLGEVD
                     RWLAECLWRRSSVNVNLIRAQAVLQRYSRPDSGKGAPRQAAETIAELEAERGRIAREL
                     HDDAGQSLAGVIVNLELAQRHLDSANSEAMERLARSRSLAASALDQIRRISRNLLPPE
                     WGDLDFGAAVENLLENMGARARLDVETGPLELPAGVLPALKTTLYRTLQEGITNALRH
                     SGATRLRIHASAPPGRVVLVLEDNGRGFDPRAAAGNKGIGLRNIRQRIESFGGSLEIA
                     AAPQHGVRLTICAPIPPKGK"
     gene            7214..7819
                     /locus_tag="VFA54_00945"
     CDS             7214..7819
                     /locus_tag="VFA54_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49398.1"
                     /translation="MKSYSPLRSAAWAVAGATLLGGGMLLGFASALVRRRELGEPEKA
                     AAGGAGALDAVYLKRIDELASAVAGIQERLDRAAPYAAPESTSEKIQAVSLRVEQLER
                     RVDQLMSEMPSVPPVDHVLAAVEHMVAAKIGGLDERLTNQVQSIELLRKASSQTDALL
                     QKLIAAVEDLSHAAEQPGRPTPQPEGAERQPPEPPENYPIA"
     gene            8217..8834
                     /locus_tag="VFA54_00950"
     CDS             8217..8834
                     /locus_tag="VFA54_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49399.1"
                     /translation="MVRIAALVAVSVISCASAQTPVEDPACSCHGSPAKVEKTDCTQA
                     GDTIAGKQAFLHAAAVTAEQEIRNSPDEWGRGIPGVGKRFASVFGKHLIKSGVQLGVA
                     RLLHENLGYCRSQKSGFRARLNHALVSAVMGRSTRTGNATPAVGRFSGAITSGFVSRT
                     WQPARLRTASGGIASAGVSLGADAGANVVREFWPEIRRFFGRRSN"
     gene            complement(8970..9539)
                     /locus_tag="VFA54_00955"
     CDS             complement(8970..9539)
                     /locus_tag="VFA54_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZS49400.1"
                     /translation="MNIDAKGKTVAVLVEDFYQELEVWYPLLRLREAGVEAFTVGLQA
                     GVTYKSKLGYPVKAERGITAIAPADLNAVVIPGGWAPDYMRRHESFITLVREMNALGR
                     PIAAICHGGWLLCSAGVLRGRRATSFFSIKDDLINAGALYQDRSVVVDGNLITSRTPE
                     DLPDFCRALLHALGVAAEEAAVVGQGAER"
     gene            complement(9536..10384)
                     /gene="rsmI"
                     /locus_tag="VFA54_00960"
     CDS             complement(9536..10384)
                     /gene="rsmI"
                     /locus_tag="VFA54_00960"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZS49401.1"
                     /translation="MTGAIYLVATPIGNLEDITLRGLRILKEAGLIACEDTRQTRKLL
                     EHYQIHKPLVSCHEHNEAERAEELVKKALAGEKIAVVSDAGMPGISDPGYRVVTRAIQ
                     AGVPVIPVPGPVAIEAAIAACGLPAESFHFGGFLPAKRGQRRKVLQSLRAETAPLVFY
                     EAPHRIVDTLEDVIETLGPRPVAVARELTKVHEEFLRGPAPVVLDELRSRSAVKGEIT
                     LIIGRGSEPSAVPAGVAGSIGRRMEELMREQALDHMTALKQAARERGLSKREAYRQWQ
                     KAKEEE"
     gene            10471..10881
                     /locus_tag="VFA54_00965"
     CDS             10471..10881
                     /locus_tag="VFA54_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HZS49402.1"
                     /translation="MFEILEHTADIGFRTYGRTPGELFEAAAEALTSIVMEREVVASR
                     IERPLSADGADYESLLVNWLSEVLYFIDAERMAFHHFRVSEITPLHVAGTGFGEPYDA
                     ARHRGKIVVKAVTYHQLKVTQTSEGWTAEVYLDV"
     gene            10881..>11563
                     /locus_tag="VFA54_00970"
     CDS             10881..>11563
                     /locus_tag="VFA54_00970"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZS49403.1"
                     /translation="MATHIEIESLDQWRHRIPRNSQIGMRTDVIVYASRALLEQIRKD
                     LSLEQAINVATLPGIVGPSLAMPDIHQGYGFPIGGVAALDCEHGVVSPGGVGFDINCG
                     VRLVSTGLSAEEARPRLRDLVNQVFRDVPCGTGGSGFLNVSEKQLDEVLERGAKWMVE
                     NGYGCQRDAEFAEAGGALEGADPSKVSDRAKRRGGPQLGTLGSGNHFLEIQYVDKIHF
                     PEAARVLGL"
BASE COUNT         2239 a         3475 c         3589 g         2260 t
ORIGIN      
        1 aatcccgaag atattcccgc attgatagaa aagctgcacg cggatggcta cgatatggtt
       61 accggatcgc gcttcctgaa gcgcaatggc tacccgattc aaccgctccg gcgcctcggg
      121 ggcctgctgt tttcggtgat cctctatccg attttgggca agcgaattac ggatcccacc
      181 tccggctttg cggcggtgaa ccggcgggcg ctcgaggttt tcagccgcag cttccctctc
      241 gaatatcccg agatcgaggc gctggtggtg ctgcaacgca aagccctgcg tttctgcgag
      301 gtgccggtca gaatgttccc gcgccgcgcc gggcgctcca cgattggttc cctgcaagcg
      361 atttactata tgtttcgcgt gctgctcgga gtgatggtca acgtgctcaa atatgagcgg
      421 cgattccaca ccggcgcgcg gcggccgccg ggcgcgtaag gtatggaacg gatccgcatt
      481 gtcgttgccg tattgaccgc gctgctcatc gtgcaaattg tgcgctcggt ccgccgcgag
      541 cacatccgcg tggaatattc catgacctgg ctgggcgcca ctcttctgct gctcatcctt
      601 tgcctctcgg ataaggccat gggcctgctc tccggcctgg tcggagttcg cgacgcttcc
      661 ctggtgctga tcattctggc gggagtgctg ttcgtgttta cgtttttccg ctattcggtg
      721 gtcgtttcca gtctgaaaga ccataacatc cagctcgcgc agaaggtcgg catgctggaa
      781 tgggagatcg aacggctcaa caagctcctg cacgccgtac agaacccgga gccagagagc
      841 cgggactcga agtgagaatc gtttacgacg gaactccgct gctgatgcgc agcgcgggag
      901 taaaaaatta ccattacgct cttctgaccc ggctgctgcc ggcgattcat ccccacacga
      961 ttgagttgtt cccgtttctc ccgggtctgg gccgtcttcg gaacgagcat tccaattatt
     1021 cgaggcctgc cacaatcctg cggctggcgg cggtgctctc cgataattac ctccggcttc
     1081 ccctaggttc ctgggcggcg cggcgggcgg acttattcca ccttacccac catcttctgc
     1141 atcctccgga gcgcggcgcc cttcgcctga cctctttggt gcacgatccg acctgcgtac
     1201 tgatgcccga gtttcacacc cccagcaatg tccggtattt tcgacgcttt gttcaggaag
     1261 tcatgccgcg gctgtccggc attatagtgc cttccgaagc cgtaaagcgg gatctcatag
     1321 aaaagctcca cgcctgccgg gagcgcatca ctgtaattcc gcacggggtg gaggagagat
     1381 tctttcaaac gccgcggcag ccggcgcgct tgccgcaggg agtgccggaa cgttacattc
     1441 tctcgctcgg cgccatggaa ccgcgcaaaa acctgaccgc attgctcgaa gcgtatcagc
     1501 tcctgcccga ggatctgcgg caggcttacc cgctcgtgat cgccggagct tcgggctgga
     1561 aaaatgaaag gctgcaacgg aatttgcaag accggcccag cgtatgtgcg ataggttaca
     1621 tcgacgcgga cctcctgccg atgctctacg cccgcgcctc cgttttcgtt tttccgtcgc
     1681 tctacgaggg ctttggaatg ccgctgctgg aagccatggc cgctggcgcg ccggtggtcg
     1741 cctcgaatgt ttcggccatt cctgaagttg taggtgaagc cggactgctg gtagatcccc
     1801 gcagcccttc ggaaatcgcc cgcgccatcg agcgcgttct ggacgaccgg gatctggcgg
     1861 aaaacctggc tgcccgaggc gcgctccggg cgaggcaatt cacctggcaa aaaacagccg
     1921 ccgcgacccg gtcgttcttt gagtgcgtcg cgggaagctg atgcaagccc gtgtgtgcga
     1981 atctataagg cttatgccac tagtttccgc acaggaactg cgctctcttt cgcaaaggat
     2041 tttcgtggcc gctggcgtgc ctcccgatgt ggcgcagcgc gtagcggagt ctctggtaga
     2101 atcgaacctg ctcggccatg attcccacgg ggtgattcgc gtgccgtctt atctcacgat
     2161 gctcggggac ggccgcatcg atcccaaagc tcagccgcgg gtggctcggg agaccgaaag
     2221 caccgcgttg gtggatggcg gctggcagtt cgggcaggtg acggcccggt acgccatgga
     2281 gctcgccgtc tccaaggcgg cgcgcaccgg tgtggcggcg gtgcagcttt accagagcgg
     2341 tcacatcggc cgccttggcg agtacgctca ccaggctgcc gaacagggca tggtcggcct
     2401 ggtctgcacc aacaaccata ccagcggcca gatcctggct cccttcggcg gaactgcccg
     2461 ccggctctct ccaagcccga ttgccatcgc cgcgcccggc ggccaggatt ttccgattgt
     2521 gctcgacatg accacgagca caaccgccga gggcaaaatc cgagtcagac aagcgcgagg
     2581 ccaaagattg cccgagggct ggatcatcga tgccgcgggc cggccttcca ccgaccccgc
     2641 cgacttttac ggtccgccgc cgggcagcct gttgcctttg ggcggcgaac tcggctacaa
     2701 gggattcggt ctcgctttta tgatcgacat cttgtccggc gcctttggcg gcagcggttg
     2761 cagccaggcg aatccgcccc agcttcgagg caacggcgtg tttttgcagg ccatcgaccc
     2821 tggcgccttc ggcgcgaagg aaagctttaa gcgcgaggtg gccgcgctcg ccgagtacgt
     2881 gaaaagctct cccgtggcgc ctggcttcga ccgcatccga attcccggag agacagagca
     2941 cgaggcccgg cagcagcgga tgcagcacgg cattgaaatc gaggaggaca cctggagtaa
     3001 gctccgggca gccgccgcgg aactgggact gaaatgatgg accgggcgga actggccaga
     3061 gccgtcgccg ccatccgttg ggctcaccgc attgatctag ggcagggaat tgtcacgccg
     3121 ggaatttggg ataccccgca aatactgaag cgcctccatc tgccggccga cttgaccggc
     3181 cagaccgttc tcgacatagg gtgctgggac ggcttctatt cgttcgaagc cgagcgccgg
     3241 ggcgccctcc gcgtgctggc gacggattcc tttacttggc agcgcaagag ctggggatcc
     3301 aaagacggct tcaatctagc ccggcaggct ctgcgctcgc gggtggaaga tctggaaatc
     3361 gatgtgcttg atctggcgcc ggaaactgtc ggagtcttcg acgtggtgct ctttttgggc
     3421 gtcctctatc acatgaagca tccgttgatg gccctggagc gcgtggcttc cgtcaccggc
     3481 cgcctgctca ttgtggaaac tgtcatcgac ctgctttgta ttcccggaaa agccttgaag
     3541 ttttaccccg gtgatgagct ggacggtgac gaatccaact ggttcgggcc cacgccatcg
     3601 gccgtggaag cgatgctgcg cgcagccggc ttccgccgcg tggagagaaa atggcctgcc
     3661 ggaagccggc tgctgctgtg gcgtgctttg agcctggtgg gcagccgcgc gacgaagcgc
     3721 gccgtgtttc atgcgtacaa gtaagcggcc ggcggttggc tatcagaggc gtgtgcgaac
     3781 cgtaacagga ataaagaact gtcgcggccg agtcgctcag ctcgcccttg gcggcacaac
     3841 cgtcgccagc cggtccattc gggcggccaa ccccgagtgt tggtctcacc agtggccgcg
     3901 tattttttgt tgagtgtcag tggtagtgga acgacgtggg cttcggttgc ggcgccgccg
     3961 caggcggcat ttccgttcct gcgactacag gagcttcgga aggcagcttg gccagggcga
     4021 tgtaccaaac gtacaaattc agaagatagg tgggcgcgag caggtaggcg cccaacgaca
     4081 tgaagtgatg cgtcacgcgc agcgcgtggg cgatggccgc gccgctcaac tgcaaaccca
     4141 gacccgaagt gattagataa agctgcttgt ttggattcca gcttttggta agcataatcc
     4201 aaagaatcgc attgagcagc acagccgcaa aataataatc ccggccgacc ttggtcatca
     4261 gcggccggaa catcttgtcc caagaatggc tgtgcgtgaa aaccacgctg agcacgcccg
     4321 tcgtaaccgc acccaaaaga agaccgaaat aaacagcgtt gcggtatttg tggcgctcca
     4381 tggccgcgcg gatcaaatgg atgatgatca tcagaatcag gaaggttccc acgaaatcgc
     4441 tgacccaata agcaagcacg tactctcggg accgcggccc gaagtagtac ttgaaggatc
     4501 cctgaacaac agtggaaagg agattgacga tcaggtaagc aaataagaaa gggtaacgcg
     4561 ctgctgaacc gcgcagcatg gtggagagca gcacaacata catccccatg caggcgaggg
     4621 cgaacaacaa ttcgtgaggc gacatgcccg gttaagtatc ctgtgcaacc acccgggtca
     4681 tgccgcccga agtccggcag ctacataccg aaccgcgcga aagggatatc atcttccggt
     4741 ggcggatccc atggcccatt cttcacgcgt accttgagaa ccggaatatc atcttccggg
     4801 ggtggatccc aaggtccgtt ctgtaagcta gcccgcaggg ccgggatgtc atcttctggc
     4861 ggcggatccc acggaccgtt ttgtagagcc acgtccagcg tttcaggcgg cgcggtggct
     4921 cctgtgagca acgcaactgc ggcggtctgt ggacccaaca cgccaacttg ttgttgggca
     4981 taggccgcca gaacaaacag cgtacaaatt gtaacacaaa tcacgctgtg tacgacgatt
     5041 tttttcatct caaactccct tggctcacgg caaccgctct gctttcaagc acctacaatg
     5101 accgctcgat cagtagccaa gccgatgtga gcgggtccga ctactcccgc tggtcccagt
     5161 ttccttactc caccattgtg aggtggagca cactctggca atggctcaga ttcccctgat
     5221 ctagctatag gatttatctt ggtctaggtc gggcagaact ggggaacggc tattcgagag
     5281 aagtgagctt gcgctgcaat gcgtatttca ccaagccggg aacgtcgtaa atgtgcagct
     5341 tgcgcatgat attggagcgg tacgtgtcca ccgttttggg gctgatgtcg aggcgcgccg
     5401 caatttcttt cgcccgcagg ccttcgacca ggaacccaaa cacctggtat tcgcgcgagc
     5461 tcaaactctc cagcgggtcc gcggccgctg gccggcgtgt cgaggccagg aacgcctgcg
     5521 gctgcaatag cggagaaata tagagacccc cctccagaat gatgcggatt gcgtccacca
     5581 ggtgccgcgc gggcccgtct ttcaacagat aggcattggc gccggtattc agagcgtcgg
     5641 tgatcgtctt ctgatcgcga ttgatggaga gcacgcccat gcgaatccgg gtgttccact
     5701 tgcgcgtgcg gcggatgacc tcgatgccgt acagcttggg catttgcaaa tcgacgatcg
     5761 ccacgtccgg ctgtagcgac ttgatcattt cgaaggcctg ttccccgtcg gccgcctggc
     5821 cgacaatctt gaaatcgggc tcggcgctca aaatggcggc aatcccttcg cggacgatgg
     5881 tgtggtcgtc ggctaaaacg atagaggtca tttgggtttc ttccttcatt tccctttcgg
     5941 aggaataggg gcacaaatgg tgagccgcac tccgtgttgg ggggcggcgg cgatctccag
     6001 gctgccgccg aaagattcga tccgctggcg gatgtttctc agaccgattc ccttgttccc
     6061 agccgccgca cgcggatcaa atccccggcc gttgtcttcc aagactagaa ccacgcggcc
     6121 gggtggagcg gaagcgtgaa tcctcaagcg ggtcgcgccg gagtggcgta aagcatttgt
     6181 aatgccttct tgcagcgtgc ggtacagcgt cgtcttgagg gccggtagaa cgccggccgg
     6241 aagctcgagg ggaccggtct ccacatccag gcgcgcgcgc gcccccatgt tctccagaag
     6301 attttcgacg gccgccccga aatccaagtc gccccactcc gggggcagca ggtttcgcga
     6361 aatacgccgg atttgatcca acgccgaagc ggccagggat ctgctccggg ccagacgctc
     6421 cattgcttcg ctgttggccg aatccaaatg ccgctgcgcc aactcaagat tgacgatcac
     6481 cccggccagc gattggcccg cgtcgtcgtg cagttcgcga gcgattctgc cgcgctcggc
     6541 ttccagttcg gcgatagttt ctgccgcctg cctgggagcg cctttcccgg agtcgggccg
     6601 ggagtatcgt tgtagcaccg cctgtgcccg aatcaggttc acattgacgg agctccggcg
     6661 ccagaggcac tctgccagcc accgatccac ctcgccaagc cgctgccgga agcgggcgcc
     6721 ccaatcgccg ggagagatgc cgagccagag ccattcggcg cctaaccggt gccgggaaag
     6781 gggcggcagc ggcttctcgg aggcaagtac cgcgagaacg ctggacctgc ctcgaagggc
     6841 ttcgcgagcg gcgtcgttca tccactggat ttccccgctc gaatcaaagc gaatcagcag
     6901 aggctggtcc tggtccacca aagcaggctt ctgcgctgct tccggacgat gccgggtggc
     6961 gggagccggg gcgaaggcga gtgccgacat acctaaggaa aatccactac cactctgcgg
     7021 ctaagccact acaagcataa cgaaaaagac atatgctgac aagcgtaaat cttaatctaa
     7081 acccctgaga tatatggtac ttatagtact cgcgaaaaac tgtagtacta tagccggaaa
     7141 agtaaccgcg aatggcagcc gggatgatat actacctgcg ccacagtcac ccccgctgct
     7201 cggcaatccg gcgatgaagt catacagtcc tttacgaagc gccgcttggg cggtggcggg
     7261 agctaccttg ctgggcggag gaatgctgtt aggattcgct tcggccctgg ttcgccggag
     7321 agagctgggc gaaccggaaa aggcagcggc cggaggggcc ggcgcgctcg atgccgtcta
     7381 tctgaaacga atcgatgagc tggccagcgc ggtcgccggc attcaggagc gtctggaccg
     7441 cgccgcgccg tatgccgcgc ccgaaagcac cagtgaaaaa attcaggcgg taagtctgcg
     7501 cgtggagcaa ctggaacggc gcgtagatca attgatgagc gaaatgccgt ctgtaccccc
     7561 ggtggatcac gtgctggcgg ccgtcgagca catggtcgcg gcaaaaatcg gcgggctcga
     7621 tgagcgcctc actaaccagg tgcagtcaat tgagctgctg cgcaaagctt cttcgcaaac
     7681 cgatgctctg ctgcaaaaac tgattgccgc cgtggaagac ctttctcatg cggcggaaca
     7741 gcccggacgc cccaccccgc agccggaagg cgcagagcgg cagcctcctg agccgccgga
     7801 gaattatccc atagcctgag gcttccctga cagttgggaa atgagtgccg cgtgtgctcg
     7861 ggccgcagct cggcgaaatc cccggcgaaa ccccatgcca cgcgagcgaa cataaccgct
     7921 cccctttgac gcctgagggc gaaccgatgc cggtccgatg acgttcattt ctgttcgata
     7981 gcaacaggcc gttcatgatc cggagccggt tgagcgggct tgcatccttt ttccacgcgg
     8041 cctgccattg ccggcgggct tgcgctgaca tccggcgcgc cggatcgttc tgaaacatcg
     8101 gccgcagtcc gcgcattggc ggaaatcggt tttggaaagc acaggttgcc cagaccgatc
     8161 cgagacgtac agcgaagtag gtgtgtggac aaccgattgc acgcaccagt agacgaatgg
     8221 tccgtattgc ggccctggtt gcggtctcag taatctcctg cgcgagcgcg cagactccgg
     8281 tggaggatcc agcttgctcg tgtcatggat cgccggccaa ggtggagaaa acggattgta
     8341 cgcaagccgg ggacacgatt gccggcaagc aggcattttt gcacgcggcg gccgtcacag
     8401 cggagcaaga gatcagaaac agcccggacg agtgggggcg cgggataccg ggcgtcggta
     8461 agcggttcgc ctctgtattc ggcaagcacc tgatcaagag cggcgtgcag ctcggcgtgg
     8521 caaggctgct gcatgagaac ttgggctact gtcgatcgca aaaatcgggt tttcgcgcgc
     8581 ggctgaacca tgcgctggta agcgccgtga tgggacgcag caccagaacc gggaacgcta
     8641 ctccggccgt cgggcgcttt tctggcgcca tcaccagcgg ctttgtatct cgaacctggc
     8701 agccggcccg gcttcgcaca gcttcgggcg gcatcgcatc cgccggcgta tcgctcggcg
     8761 ccgacgcggg cgcgaacgtg gtgcgggagt tctggccgga aatccggcgc ttcttcggcc
     8821 gccggtccaa ctgacgtgtg cgggaacgac tcatggtagc cgagtttcca aatatatact
     8881 ggcgtggcga gcaggacgga gcaagggcga gctgccgcgt caggggaaga cgcccggcgg
     8941 cgtccggcga ggcacgtccg ctccggtagt catcgttccg cgccttgccc gaccaccgcc
     9001 gcctcttccg ccgctactcc gagcgcatgg agcagggcgc ggcagaaatc cggcagatcc
     9061 tccggggtgc gcgaagtgat gaggtttcca tcgacgacca cagaacgatc ctgatacagg
     9121 gctccggcgt tgatcagatc gtccttgatg gagaaaaatg aagttgcccg ccggccgcgc
     9181 agcacgccgg cggagcagag cagccagccg ccatgacaga tagcggcaat cggacgtccc
     9241 agcgcgttca tctcgcgcac gagggtgatg aagctctcat gccgccgcat gtaatcggga
     9301 gcccagccgc ccgggatgac cacggcgttc agatccgcgg gtgcgatagc ggtgatgccg
     9361 cgctccgctt ttaccgggta acccagtttg cttttatagg tgacgccggc ctgcaagccg
     9421 acggtaaagg cttcgacccc cgcttcccgc aggcggagca gggggtacca aacctctagt
     9481 tcctggtaaa agtcttcgac caggacggcc actgtcttgc ctttggcgtc gatgttcatt
     9541 cttcctcctt cgctttttgc cactggcggt acgcttcacg cttggaaagg ccccgttcgc
     9601 gggccgcttg cttcagtgct gtcatatggt cgagcgcctg ctcccgcatc agctcttcca
     9661 tgcgccgccc gatcgagccg gcaacgcccg cgggcacagc cgaaggctcc gaaccgcggc
     9721 cgatgatcag cgtaatctcg cctttgacgg cggagcggct gcgcagctcg tccagcacca
     9781 caggcgccgg gccgcgcaag aactcttcat ggactttcgt caattcccgc gccacggcga
     9841 cgggcctggg tccgagcgtt tcgattacat cttccagtgt atccacaatc cggtgcggcg
     9901 cttcataaaa taccaacggg gcagtctctg cccgaagtga ctgaagaacc ttccggcgct
     9961 ggccccgctt cgcgggcagg aatcccccga agtgaaacga ctcggcgggc aggccgcaag
    10021 cggcgatagc ggcctcgatg gcgaccggcc cgggtacggg aattacaggg acgccggcct
    10081 gaatggccct ggtgactacg cgatagccgg gatcggagat gccgggcatg ccagcgtcgc
    10141 tcactacggc gattttttcc ccggccagag cctttttcac aagctcttcg gcgcgttccg
    10201 cttcgttgtg ctcgtggcag ctcaccagcg gtttgtggat ctggtaatgt tcgaggagct
    10261 ttcgtgtctg gcgggtgtcc tcgcaagcga tcaagccggc ttctttcagg atacgcagcc
    10321 ctcgcagggt gatatcttca agattgccga tgggcgttgc gaccagatag atggcgccgg
    10381 tcatgcaaag tcatccaacg tccgcgagca ctcgagcaat tgtccgctgg acttgcccga
    10441 ggcgggcaac agcttctagc tattgtagaa atgtttgaga ttctggaaca caccgccgat
    10501 atcggctttc ggacgtacgg gcgtacgccg ggggaattgt tcgaggcggc ggccgaagct
    10561 ctgacttcga tcgttatgga gcgggaagtg gtagcttccc ggatcgagcg cccgctttcc
    10621 gcagacggcg ccgattacga gtcgcttctc gtgaactggc tgagcgaggt gctgtatttc
    10681 atagatgcgg agcggatggc ttttcaccat tttcgtgtga gtgaaatcac cccgctgcac
    10741 gtggccggaa ctggttttgg agagccgtac gatgcggcgc gccatcgtgg gaaaatagta
    10801 gtcaaggcag tcacctatca tcagttgaaa gtcacgcaaa cttcggaggg atggaccgcc
    10861 gaagtttacc tggatgtctg atggcaaccc acatcgagat cgaatccctg gatcagtggc
    10921 ggcaccgcat cccgcgcaat tcgcagatcg ggatgcgcac ggacgtcatt gtctacgcca
    10981 gccgcgcgct gctcgagcag atccgcaaag acctctcgct cgagcaggcg ataaacgtgg
    11041 cgactctgcc gggcatcgta ggaccgagtc tggcgatgcc cgacattcat caaggatacg
    11101 gtttcccgat cggcggcgtg gccgccctcg attgtgagca cggcgtggtg tcgcccggag
    11161 gagtcggctt tgacatcaat tgcggcgtcc ggctggtgtc aactgggctc agcgcagagg
    11221 aggcccggcc gcggctccgg gatctggtaa atcaagtctt ccgtgacgtg ccgtgcggca
    11281 cgggcggctc gggctttctg aacgtcagtg aaaagcaact ggatgaagtt ttggaacgcg
    11341 gggcgaaatg gatggtggag aacggctacg gctgccagcg cgatgcggaa tttgccgaag
    11401 ccggaggggc tctcgagggc gccgacccaa gcaaggtgtc tgaccgtgcg aagcggcgtg
    11461 gcgggccgca actcggcacg ctgggcagcg gcaaccactt cctcgaaatt cagtacgtcg
    11521 ataaaatcca ctttccggaa gccgcgcgag tcctgggact cga
//
LOCUS       DASREK010000015        83610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_175620, whole genome shotgun sequence.
ACCESSION   DASREK010000015 DASREK010000000
VERSION     DASREK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 83610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83610
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175620"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /gene="amrB"
                     /locus_tag="VFA54_00975"
     CDS             complement(<1..181)
                     /gene="amrB"
                     /locus_tag="VFA54_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZS49404.1"
                     /translation="MLRRAAWAGQFYPGDPSALRAELQHLLAGAPSKEIAAPIGCVSP
                     HAGYMYSGAIAGAVYR"
     gene            complement(248..526)
                     /locus_tag="VFA54_00980"
     CDS             complement(248..526)
                     /locus_tag="VFA54_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49405.1"
                     /translation="MDERLKQLMQELGNAINESLSDSEAIAQAIGEIKQAGYDVFLVL
                     EATIGFNKRSSAEEVQREEEETSAPQTVAITLTRQDQEFLKAMKIVAE"
     gene            732..1013
                     /locus_tag="VFA54_00985"
     CDS             732..1013
                     /locus_tag="VFA54_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49406.1"
                     /translation="MLDLRAAAATTRSLAEVVRQIWKALRILFQEVLGFTFFVLGGWG
                     VLWLVRSYHRFQGDGESVFKMAVVALFVLMMGACGFSSFRRARRIGRGK"
     gene            1023..2609
                     /locus_tag="VFA54_00990"
     CDS             1023..2609
                     /locus_tag="VFA54_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZS49407.1"
                     /translation="MEQHRKARFTSSGIEIPICYDPRAIDYDAQLGNPGEFPFTRGVQ
                     PDMYRGRLWTMRQYAGYGSARESNARFRYLIAQGAKGLSVAFDLPTQMGLDADHALAL
                     GEVGRAGVSICSLADMQELFQDIPLERVSTSMTINSTAAILLALYVAVARQQGCDLRK
                     LAGTVQNDILKEYAARGTYIYPARASMRLVTDLFAWCHREMPDWNPISISGYHFREAG
                     ATAVQEVAFTLANAVAYIEAAVGAGLEIDSFAPRLSFFFNAHSHFLEEIAKFRAARRL
                     YARLMKERFRARNPRSWMLRFHAQTAGSTLTAQQPDVNIIRTAIEALAAVLGGAQSLH
                     TNGFDEALALPTEESARLALRTQQVIAHESGVANTVDPAGGAYAIEALTDEIEARASE
                     LMRRIDAMGGMVSAIEQGFVQREIQNAAYQYQKQVESGERVVVGVNRYMPEKETPIPI
                     LRIDPAIERAQVDRVRQLRAARDNARADKALDEVERTASSNDNLLPAILAAVESYATL
                     GEISDRMRRAFGEYRETVVW"
     gene            complement(2767..3054)
                     /locus_tag="VFA54_00995"
     CDS             complement(2767..3054)
                     /locus_tag="VFA54_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49408.1"
                     /translation="MKLSFFPGKLFLESKPDGSYEMRFGEEVQTFRSEKMAVQAFQQI
                     RTKLEKDFPAREPTMEERRALLQKEIADSALGHSPMRNAGPRKKTGTRTFG"
     gene            complement(3077..3361)
                     /locus_tag="VFA54_01000"
     CDS             complement(3077..3361)
                     /locus_tag="VFA54_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZS49409.1"
                     /translation="MRTIALLLTLPLWCHAVLAAAKQPLTDDVLYDRVIRALVNDRDL
                     KTNSIEVSVKDRVVTLRGVIDSEKLRQRAERVAQKVDGVKKVINELRVRP"
     gene            complement(3358..4269)
                     /gene="era"
                     /locus_tag="VFA54_01005"
     CDS             complement(3358..4269)
                     /gene="era"
                     /locus_tag="VFA54_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZS49410.1"
                     /translation="MKSGFVSILGRPNAGKSTLLNRLVGAKIAIVAEKPQTTRETIQG
                     VVTRPEAQIIFLDSPGIHEPRNTLSRRMMREVRAALEARDLLLLVVDATVAFGPGDEQ
                     ALELIRSAGTPALLALNKIDRLADKSLLLPLIERYKQSFAFEEYLPLSALTGEGVPEL
                     LDALIARLPEGPQYFPPDHLTDQPVRHLAAELVREKVIWHTRQEVPHATAVVVEDFQE
                     SEKLLRIAATIYVEREGQKGIIIGSKGERLKHIGTEARQEMEMLFGRKVFLELHVKVR
                     RDWRESESFVEALDWRRQLESRAGKQI"
     gene            complement(4266..5552)
                     /locus_tag="VFA54_01010"
     CDS             complement(4266..5552)
                     /locus_tag="VFA54_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZS49411.1"
                     /translation="MTPAFAILVVIALCAALVLASYFHLLYSESLRIMPREAARSLPY
                     FEDALRPKLKLEAESGMQRFAAVKQCILLLLAFDLAYLRGRGGFTGARLLEALVLAGV
                     LLILFAYIAPHILVTRTTGRWALRFLAVARALSLAVRPLLLVVGFGYSIADLGVESKA
                     EEAPATPEENIEALMDAGQEEGLLGEDDRKLIQSVVEFGDKTVREVMTPRPRIVAIEA
                     SASLEELRQLLINERYSRIPVYKGTIDAIEGHVHVRDMLEVEESERQSRRVGELLRPV
                     LFVPETKRVNDLLREMQEKSLQMAIVIDEYGNTAGLATMEDLVEEIVGEIRDESEPVS
                     DVIEEPDHSLVVSGDLDVDRLKDLTGYRPEQDLESTTIAGLVTERMGRVPAPGEKLQL
                     NGIQIEVLASNGLRVEKLRLRRTGQEESSAELPYTA"
     gene            complement(5549..6148)
                     /gene="ybeY"
                     /locus_tag="VFA54_01015"
     CDS             complement(5549..6148)
                     /gene="ybeY"
                     /locus_tag="VFA54_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZS49412.1"
                     /translation="MPPSEHSSGDSDPADWFSNRQTAVGFNRSDLIRFASELRRRLAG
                     GREFGLTIASDAAVRRANLRFRGENETTDVLSFPDGEGMYLGDILISAAQARRQALAL
                     GHSIEDELKVLLLHGLLHLLGYDHERDGGRMERHERHWRRRLRLPSGLIERAGSRHDP
                     DEGSSREARMKPARARPSGTLNSLKKRPRKYKHTGPPKR"
     gene            complement(6157..7218)
                     /locus_tag="VFA54_01020"
     CDS             complement(6157..7218)
                     /locus_tag="VFA54_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZS49413.1"
                     /translation="MKRQVKITRGIEPLFGTRDENIRLLESALSVSAHLLEDSLEIEG
                     EPERVRRAESILEDYVYLVQQGQVFTNGDMKSYLRVAVEDPQVSLRNLVASAKQRNFG
                     KKSLAPRSVNQMRYVEAIERNDMVFAIGPAGTGKTYLAVAMAVSALLSKRVSRIILAR
                     PAVEAGERLGFLPGTIQEKVDPYLRPLYDALFDMLEVEKVEKYLERGLIEVAPLAFMR
                     GRSLNESFIILDEAQNSTPEQMKMFLTRLGFGSKAVITGDVTQIDLPAGKRSGLLEAI
                     QVISGVQGVSFVYLDERDVVRHALVQRIIKAYEQYGDAAGRQLSFPLDHPAPPGGTPQ
                     TESDAAADPPSSPAAEQAS"
     gene            complement(7818..8576)
                     /locus_tag="VFA54_01025"
     CDS             complement(7818..8576)
                     /locus_tag="VFA54_01025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS49414.1"
                     /translation="MAGSGKTAVVTGAGSGIGRAAAVALQSAGYSVVLAGRRAEALEK
                     TAAMATPSGGRMLVVPTDVNDPEAVRALFAKTREAFGRLDLLFNNAGANAPGIPMEDL
                     TYEQWSAVVGVNLTGAFLCAQEAIKMMKAQNPRGGRIINNGSISAHAPRPDSAPYTAT
                     KHAITGLTKCISLDGRKYDIACGQIDIGNAATEMTERMTKGVPQANGSIHVEPRMDVR
                     HVAEAIVFMASLPLDANVQFMTIMATKMPFVGRG"
     gene            8771..10807
                     /locus_tag="VFA54_01030"
     CDS             8771..10807
                     /locus_tag="VFA54_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZS49415.1"
                     /translation="MATAFQEQHIIEFDRHACGDLEFALQREWLETNGLGGFASSTIA
                     GCNTRRYHGLLIASITRPAGRFVLLSKLEETLILDGRRFELSVNRYRPGVIHPRGHRY
                     LRRFRLDPFPIFTYGAEGVEIEKSLFMIHGENSVIVQYQMSQAEGAPEHHECLLELRP
                     LIAFRDYHGLTYENGVLDPQVGMEPGIACVRPYPGLPALYISHQADVVDPAGYWYRSF
                     EYERERERGLDYWEDLFSPFAARVDLKRQPAPALIASLERRAAATAQALRQSEIERRR
                     GIANASRCEQPFVRALAAAADQFIAARGDRKTIIAGYHWFSDWGRDTLISLPGLTLVT
                     GRPEIARDVLIGMARYADRGMLPNRFPETGAAPEYNTADASLWFFEAVRAFLDSQEDY
                     DFVEAGLYKALTDIIAWHVRGTRYGIHVDSDGLLASGAPGVQLTWMDAKVGDWVVTPR
                     CGKPVEIQALWYNALRIMEDLARRFRDHAGAAEYARMADLANRSFNAQFWNEAEGCFY
                     DVINGAERDGSMRPNQVIALSLPHGMAPRERCVRALDAIERDLLTPYGLRTLSPRDPR
                     YRGRYEGGPSSRDAAYHQGTVWPWLMGPFLTAYIKAHGRTSEVLGRAAAWLQPFAQHL
                     SEAGLGQISEIFEGDFPHRPCGCIAQAWSVAELLRAAAQDVFGVSPAQAAPREN"
     gene            10928..13804
                     /locus_tag="VFA54_01035"
     CDS             10928..13804
                     /locus_tag="VFA54_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZS49416.1"
                     /translation="MAPQIIEQSWQMRLRSWADVQRVRQHENKLLLILTLVIGAVVGL
                     VVVAFIWLTENLGARMYPAGGAAWRRVFVPTAGSLIAGFLLDRYFPNARGSGIPQTKA
                     ALFLRDGYIRFRTVLGKFACSSLSLASGIALGREGPSVQVGAGIASVLGRRLGLSSDK
                     IRSLVPVGTSAALAAAFNTPISAVLFTLEEILGDLHAPLLGSVVLSSATAWIVLRLVL
                     GDEPLFHVPAYQLVHPLEFGIYAALGVAGGFVSVGFVKLLLFLRKRFLAMPRRTSPFQ
                     PAAGGVLAGLLGWFAPEVLGVGYGHVSSALNGQMSLGLMALLAPLKVLATAACYASGN
                     AGGIFGPSLFIGAMTGGAVGTAAHYFLPDYTGSVGAYALVGMGTAFAGIIRAPLTSVI
                     MIFEITRDYSIIVPVMLSNLISFFISHRLQEEPIYEALQHQDGIHLPAGGGRHEGILL
                     VRDAVRQPVKALSAEDRLSDRIEIIESREADAWPVVNERGLMGMVTAAQVRQAIEQGN
                     GARTFGDLVPDPEAMRPLRAENFPHLHADQSLDLALRRMAESGLTVLPVVSRTNLREL
                     QGTVSLNDALTAYGLGKDRKPPAQPAVKSPEPSVPLASFLAVLVGILVLASFFTYLYR
                     AQRRERAAREFQQGTELARQGRDQEAIENYRHALSVSHSREHRLALALELVKVGRWNE
                     ARSYLQELLREDPASGVVHLAMARIEAGEGRTQEAVTEYNRAIYGSWAAAVEGPVQAR
                     FELADLLARTGAKQQAAAELIKLLGETPESDFAARKKIARLLLDYGSARQSAAILREV
                     LSRDSQDPEAYAELGAAEFALEDYAAAKSAYGHAARLNPSDAAAQARLGLCNEILALN
                     PRARSLRAAERYARSKNLVQRVLQAAGPCIASRGNVPLPDDIRRLSQKARQLLASRSG
                     PRSYRDAADDNIALAGQLWEARNRFCASPPPDEALSRVLALLAH"
     gene            13927..14130
                     /locus_tag="VFA54_01040"
     CDS             13927..14130
                     /locus_tag="VFA54_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49417.1"
                     /translation="MSNVAYVSRIHIERRAGPLRIAHLPGERQPVVFSVHGAIAEHYK
                     MDPAAFAEPHASTIDYVIAAAAG"
     gene            14131..14403
                     /locus_tag="VFA54_01045"
     CDS             14131..14403
                     /locus_tag="VFA54_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZS49418.1"
                     /translation="MLGTFGGALEARRIDAGNGRLTAEVTGEVETDEGVLVIRRIHVA
                     MKLAAPVQSRETVERVHGTYAMRCPLYRTLHKAIELTSSYTLVEEQ"
     gene            complement(14472..15929)
                     /locus_tag="VFA54_01050"
     CDS             complement(14472..15929)
                     /locus_tag="VFA54_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HZS49419.1"
                     /translation="MAYQDLRDFLRALHKQGELKRVSEEVDPILEITEIADRAVKKGG
                     PALLFERPKGYDIPVAINLLGSQRRMELALEVDSIEDVAGRISEYLAMKAPQGVLGKI
                     KMLPKLAEMGSFFPRTVRGGPVKEVIQREKFSLLDFPVLQCWPKDGGRFITMPLVFSR
                     NPDTGKRNCGMYRMQIYDDRTAGMHWQTHKQGAEHYRRSLKNIQKEAGARMPVAAAVG
                     SDPATMFSAVLPLPPDLDEMLFAGFLRSEPVEMTPCETIDLEVPANAEIVLEGYVNLG
                     ELRTEGPFGDHTGFYSLEDDYPVFHVTCVTRRKKPIYATTIVGPPPMEDFYMGNAIER
                     IFLPLMRLQLPEVCDIHMPAEGVFHNLMLVSIRKSYPGHARKVMSAIWGLGQAMFSKC
                     IVIVDEDVDVQNVREVAWKALNNIDPERDIQFVLGPVDSLDHSSRLPNYGSKMGIDAT
                     RKWKEEGFLRPWPEPIVMDPAVKKRVERLCKQLGL"
     gene            complement(15932..16540)
                     /locus_tag="VFA54_01055"
     CDS             complement(15932..16540)
                     /locus_tag="VFA54_01055"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS49420.1"
                     /translation="MTVRTAAFSRPPSLLFVLMLAFSASAAAAWKPLFNGKDLSGWEQ
                     VGKAVWRVEEKMIVGSSDPANPGTGWLVTRREYSDFRLRLKFWITPGGNSGVAIRDPS
                     HARGARTPAHLGYEIQILDVPHEKNPTGSIYDLAPARDGKIKSGQWNEFLITCKGPRI
                     EVMLNGEKVAETANNRSRRGAIGLQMHSPNMTVKFKDLEIEE"
     gene            16570..17505
                     /gene="fabD"
                     /locus_tag="VFA54_01060"
     CDS             16570..17505
                     /gene="fabD"
                     /locus_tag="VFA54_01060"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZS49421.1"
                     /translation="MSKTAFLFPGQGSQTPGMGRELAERFPSAREVFERADAALGFKI
                     SQLCFEGPEEALRLTANTQPAILTCSIAAWAVLREHGVAGDYVAGHSLGEYSALVAAG
                     SLALEDAVKLVRRRGQYMQEATPEGVGAMAALLGLPNGALAGICAEAAGGEIVSPANL
                     NSPDQVVIAGHKGAVERAVLLAHRAGAKRAVMLPVSAPFHCRLMEPAQQRLAADLAAI
                     PFRDLQMPLVNNCAAREISTGDEARQGLIDQVPNPVRWEETIRYMAARGVSRFIEVGP
                     GRVLCGLLRSIDRSLKGFNVEDVKGLEKLLAAEYV"
     gene            17571..18305
                     /gene="fabG"
                     /locus_tag="VFA54_01065"
     CDS             17571..18305
                     /gene="fabG"
                     /locus_tag="VFA54_01065"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZS49422.1"
                     /translation="MQNRVAFITGASQGIGRACALALARRGARVIAAARQQDKLEQVV
                     TEICNAGGRAAAVPLDVTSGESVASAFRQAEALFGRIEILVNNAGVTRDGLVMRMKRE
                     NWDAVLNTNLTGSFLCIQQVLPGMVRARWGRIINISSVVGETGNPGQANYVAAKAGLI
                     GLTKAVAQEVASRGITVNAVAPGFIDTAMTAALEPAARERLLPLIPLGRMGTDMDVAH
                     AVCFLASDEAGYITGHVLDVNGGLRM"
     gene            18357..19574
                     /locus_tag="VFA54_01070"
     CDS             18357..19574
                     /locus_tag="VFA54_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HZS49423.1"
                     /translation="MKRSGCADLPLHGGRVPAWLAGRMTKLGAAIAESVLYDYGPSAL
                     LSRLSDPFWFQALGCVMGMDWHSSGITTSVLGALKRGLNPRSRELGITICGGRGRHSR
                     NTPAELRAVAEHTGLDGDALVRASRLTARIDNNAIADGFQIYLHAFVVTRTGEWAVVQ
                     QGMNERSGLARRYHWHSTGVRDFTEEPHTAIVGGAIQNRIMNLVDREAKAAQNALLAI
                     VRERPEVTIAEARKLCAPKHHDVRAEDVDLKRLGAVLAAAYEQEFRDFASFLLLENLG
                     PRTLQALALIAEVVHGTPSRFEDPARYSFALGGKDGHPFPVPLGTYDESIAVLRRSLD
                     AAKLGHTEKLAGFARLDRFVRMVEENRSPQAQFEALIAHEQAISPRLGGRSVSHGPKI
                     TAPTAAPKQLRLF"
     gene            19746..20858
                     /locus_tag="VFA54_01075"
     CDS             19746..20858
                     /locus_tag="VFA54_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZS49424.1"
                     /translation="MKKKLLTVLAFTGGIFLALEAGARIFSSDGRAGILSQHPGLAMY
                     AGIERPEEVFSLLNPGCLEWSPYQHWVVRPNLRSRFYRTNALGLRGLETAMEKPDGRF
                     RIVVLGGSAAWGLGSTADERSLPGRLEAHLRERNPSRELEVINAGQPGYTSGQELIYF
                     HRLISRMNPDLVLLFDGYNDIDADLHNSQPGWPENANILRARYDASFSKTRLSQEFLD
                     LLRRSRFLHLASQKLGAFSPAGEAPGETLFAPEVTAMNYVHNVKALARLASPAPVWVA
                     LQPVLATVRKPLAPEEREILAAKEKIIPDYTERVRRTYQGIEEQVRAAAIPFLDLNAA
                     LGEQPRLLFADECHFGDEAADDLARRIAAACERLIQ"
     gene            20953..21246
                     /locus_tag="VFA54_01080"
     CDS             20953..21246
                     /locus_tag="VFA54_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49425.1"
                     /translation="MELWIIDPGGGLCEAHVLLFATAWAAPASSGAINTLTPQESAQD
                     WKLLWDKKTLDGWKINNGKNVLDAHHSAFQRGAIGLQHTGQKIEFRNIKIRPL"
     gene            21426..22085
                     /locus_tag="VFA54_01085"
     CDS             21426..22085
                     /locus_tag="VFA54_01085"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS49426.1"
                     /translation="MKRAYLLTVAAAAALAAAALFAAPAGSGAVNTLTPQEASSGWKL
                     LWDGKTLNGWKQYGGAQWKVENGALTPSGRYGWLGTEGDYDNFMLKVDFRTSASNLNS
                     GIFLRRGRDEKDSHQIGYELQIRDPQPNDKPYDGKPDNHNGYYTGSFSGHLKSHNEPK
                     IVLNEWHTFEVTAQGDHFVVLYDGAKVLDAHHSAFKNGAIGLQYTGQNVEFRNIKIKP
                     L"
     gene            22409..23149
                     /locus_tag="VFA54_01090"
     CDS             22409..23149
                     /locus_tag="VFA54_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS49427.1"
                     /translation="MRGEKIVLDDLTFRVDIGEHVAILGPNGCGKSTLIKTITRECYP
                     LQREGSRMSIFGKETWNVFDLRSLLGIVSNDLMSQCTRDITGRDIVLSGFFSSIGVWP
                     HHQVSAAMREKADEVLEILEAPHLAGRPVNEMSSGEARRILIARALVHDPRALVLDEP
                     SNSLDVCAQHELRQVMRKLAQSGIAILLVTHHLADIIPEIDRILLMREGRIVADRPKD
                     RILKAECLSDLFGVPVELGRRDGYYHLW"
     gene            complement(23276..24295)
                     /locus_tag="VFA54_01095"
     CDS             complement(23276..24295)
                     /locus_tag="VFA54_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS49428.1"
                     /translation="MQGSPAAAPTPALIFETFNAYQKTAALRAAIELDMFTGIGEGAA
                     TPKALAERCRASERGVRILCDYLVIAGLLSKDRGRYSLTQDSALFLDRNSPACMASAV
                     RFLNSPDLINAFQDLAAAVRNGGTAMSPDGTIGPENAVWVEFARSMVPLMAPAAEEIA
                     GLIGADAGRKCTVLDIAAGHGIFGITIARRNPNAEVAAVDWGSVLEVAKENAERAGVA
                     SRYRTLPGSAFDVDFGSGYDVALLTNFLHHFDPPTIENLLRKVHRSLNEGGIAVTLEF
                     VPNEDRVSPAIPASFSLIMLATTPTGDAYTFSEFEEMFRAAGFRHSEIRSLERSPEQV
                     IISRK"
     gene            complement(24476..25705)
                     /locus_tag="VFA54_01100"
     CDS             complement(24476..25705)
                     /locus_tag="VFA54_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS49429.1"
                     /translation="MKSPINLVWILALVSLSSAAAQEAPIVIKAGSAFDGRGKVLRNA
                     SIVIQGSKILRIDSKGIPGAATYDLSGLTVMPGWIDTHVHLTWHFDKNGRLAQPDRDT
                     PQQDILYALGNAYADLLAGFTTVQSLGSPMDRDLRDWINQGSDPGPRILTSLGAIDEA
                     TGGPDQIRAFVRKVSAEGADVIKMFATKSIRDGGAQSLTDEQIQAACAEAKAQGKRIV
                     VHAHASAGARASILAGATSIEHGVFLDEGVLRLMAERGVYFDPNFLVLHNYLDNKPKF
                     LGIGNYTEEGFAYMQKGLPLVAEVLRKAAAEKVKIVFGTDAVAGVHGRNYEEFIYRVK
                     DGGQKPMDAMISATSLAAESLNLGGQIGSLAPGMQADLVATEGNPLEDITAVRRVVFV
                     MKAGKIYKNTVPSRKAR"
     gene            complement(25910..27229)
                     /locus_tag="VFA54_01105"
     CDS             complement(25910..27229)
                     /locus_tag="VFA54_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZS49430.1"
                     /translation="MDQSCSPQLVRAVGLFSLTALALNGVIGAGIFVLPATVAKFLGR
                     TSPLGYIICGVATVLIVLCFAEAGSLFETAGGPYVYAREAFGGFIGFEVGWMFLLGRL
                     TGAAAVSNAFADYLGFFWPAVASGPGRSAAITLLMGALALVNVLGVRYGAWTINLLTI
                     GKLLPLLVFIAAGLFFADPRSYSFSELPDLTSLRSAALVLMFAFGGFEYAAVPSEEVI
                     NPRRNLPIALVSAIALVVVIYLLIQIVALGTLPGLGTSPAPLAAAGERFLGALGAALL
                     TLGAVMSTTGNSSAQLLVGPRILYAMAEGKQLPALLGRVHARFHTPYVSMILFALTAW
                     GMALFSNFSQLAVLSAIARLLYYLTTCLAVPVLRRKMPAAERRVTLPGGAVIPALAVA
                     VSIWLLTGSSRSQAAAGGIALLAGGALYWSYAGYKAAASKAKSPAGA"
     gene            complement(27384..27746)
                     /locus_tag="VFA54_01110"
     CDS             complement(27384..27746)
                     /locus_tag="VFA54_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HZS49431.1"
                     /translation="MSAATSALGALLSYSCPRCRRGPIFRGSLLQGWLAMHQRCPVCG
                     LEYEREEGYFVGAMIVSYVLSIPPVLALMSVFWMLTNWSLQRLLVAAFLAYLPFVPEM
                     VRFSRVVWIHMDRAFDPE"
     gene            27928..29457
                     /locus_tag="VFA54_01115"
     CDS             27928..29457
                     /locus_tag="VFA54_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZS49432.1"
                     /translation="MELTRRKLAHTAALGASAALLGGKKAKAQAPGEGASMMAASSGK
                     LARADGLTRQVAEFIVRTGYTDLPQEVIDLGKKSILDGLGLALSGSVAETGGLTREYL
                     QSLGLGRSNATVIGSPIKAPVRFAAFANGIGIHADDYDDTQLAVAKDRVYGLLTHPTA
                     PALPPALASAEAAGLPGRDLMLAYHVGVEVECKVAEAISPRHYEDGFHSTGTCGCIGA
                     AAAIAKLRGFNVERTLRTLGIAASQAAGLRENFGTMTKPFHAGRAAEAGVIAADLAAL
                     GWTAADQILEAPRGFFHAAGGGFDPNAISGKLGNPWTFVSPGVSIKPHPSGSLTHPGM
                     TEMLFLIQEHRITPQQVERVDVGTNRNMPNALIHHQPTNALQAKFSMEFCMAALLLYG
                     KAGLSEFTDEVVNRPEVQAMIQRVHFGVHPEAERAGYNKMTTIIDIHLKDGRTISGRS
                     DFGKGSPEKPMSFDEVAAKFLDCAAFANWPAEKARAVVELVRKLEDVSNVRALTALLS
                     A"
     gene            complement(29517..32780)
                     /locus_tag="VFA54_01120"
     CDS             complement(29517..32780)
                     /locus_tag="VFA54_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49433.1"
                     /translation="MQKLLSVLRLLFPPALVSATIVICWGQLQTGLTGEVSDSSHAVI
                     AGARVTVRNMNTGVELATETNAAGIYNFPVLAAGEYELGCEATGFKRYFQRGVTLDTG
                     FFRRLDIELQVGGVTESVEVTSSAPLLETETASVGQLVERQTVSNMPLESRRSAGLVR
                     LMGSVVFLQTQTVASQPVFSMSGGRSVNQMWALDGTSGQNVTMSVQQTTLNPPAESVQ
                     EFKAEENNYSAEYGRTGGGYIVITTRSGTNQYHGAAYEFFRNQSLAARTFFAKQKPPL
                     VYNIFGASLGGPIKKDRTFFFANYEGARRRDGVTYNDVVPNPPEVTGDFSNRRDLVLR
                     DPTTGQPFLNNIIPPARIDPVGRKIAQLYPPPNVPSPSTQAPKNNFINGGSNATTQDW
                     LTAKVDHNFRATDRVFVRFIFNRAPGTTTAVFPNAFADPRGNSQQNQETNTTGNWIHN
                     FSPTTIQDVRINFGRRKFVLTEFGVNSGENGKLGIRGVDPNYFGSFDINGLSSIGTTQ
                     QERIQTPIDTWQFLDHYSISRGRHLIKLGGEYRFGLNVDLFNGYKGGHFVFDNRATGS
                     GLAELLLGNVATGEVQAIVPLASRSDYYAGFVQDNWKITNKLTLNLGLRYELNQPRWE
                     RHNYQSGFNLTAINPVSGTPGIVTFAGVNGVSKYAHDFDYHDWGPRFGFAYSLGGGFV
                     LRGGYGISYYNAYQVRVTFALKAGFGISTSFNSPDGNITPAFQLQNGMPSIMPAPHDA
                     SFGAVPVGSPVILSPDFLQQDQPDPMAQQWNFGIQKQLSANSVAEITYLANVAHRLGG
                     PDVNINMIPLIKGKGPAVQSQTARPFPQFDNVMQISPAWGNSTYHAMNLKVEKRYSSG
                     LNLLANFTWSKFIDDVSANSEIGGAAGNVSYTHIALHRLDKSLSGNHIGRRLVGSVVY
                     DLPWGTGRPYLLKNRFVNGALGGWGIGLIGEIRDGAPYGAIENTNLSNTFSASQRPNL
                     LGPPLQPAGWRSNVLAIPYFDVGQFQAPGVGVFGNSPRTICCGPGFLGIDMSVHKNWP
                     LTERFKLQVRGDFYNLPNRPEFANPNLKRGLPNFGLVTGVVVGSNARLAQISMRFEF"
     gene            complement(33243..33767)
                     /locus_tag="VFA54_01125"
     CDS             complement(33243..33767)
                     /locus_tag="VFA54_01125"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HZS49434.1"
                     /translation="MARLHMLPVLAAAALLGLPAMAQQRVSPHEQTSVTIDGKKITIE
                     YGRPYMKGRKIFGGLVPLGEVWRAGADEATVLTTDADLTIGSLRVPKGSYALFVIPQA
                     NAWTLVVNKVAKQWGAFNYDQSKDLGRTPMKVTTSGSPIEQFTISLETAGPKRGVLKM
                     AWERTVATADFSVD"
     gene            complement(33806..35398)
                     /locus_tag="VFA54_01130"
     CDS             complement(33806..35398)
                     /locus_tag="VFA54_01130"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZS49435.1"
                     /translation="MRLVWLLAALRLPIPAQFSYERILRAVSDPANWLTYSGDYSSHR
                     YSALAQITPANVSGLRPVWMYQVNDPNKFETTPLAADGLVFITEPPSNVAALDARTGR
                     PIWRYARAIPDDIHICCGQVNRGLAGLGETVFVSTLDDHLVALDAKTGRVRWDVTAAD
                     YKSGYTMTAAPLAVKDKIIVGVAGGEFGVRGFLDAYDAKTGKRVWRFWTVPAPGEPGN
                     ETWAGESWKTGSATTWLTGSYDPDLNLIYWGVGNPGPNYNGTSRAGDNLYSCSLIALD
                     ADTGRLQWHFQFTPHDTHDWDANQIPVLVDRAFQGRPRKLVVTGNRNSFYYVLDRQTG
                     EFLLGREYAKQTWAKGLDVRGRPIALPNTEPSPEGSGVYPSLHGGTNWFSPSYSPQTS
                     LFYVAVREESTIFFRGEAIYQPGALYVGGSFRGLPGVEPTGYIRALEVETGRKRWEFH
                     LQSPPWGGVLSTAGGLVFGVANEGYLFALDAATGKPLWRFPAGGAIFANPITYLSDGK
                     QHVAVAAGHALISFALAENVTR"
     gene            complement(35416..36273)
                     /locus_tag="VFA54_01135"
     CDS             complement(35416..36273)
                     /locus_tag="VFA54_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS49436.1"
                     /translation="MRWISFLTLAICWGHLLGWAQVVSSPLPPSALQPPPPPPPAPFP
                     TAEDLEEGKRLFLSNCAPCHGPNGDGGKGSDLTRPHLPRASDDRIFFSVIRRGIPGTE
                     MPNTRHLLDRDIWQVIAYVRTLQRSSPPVHGDPVRGAELYRGKGKCGQCHMIAGEGGR
                     LGPELTDIGERRSPGYLRTAITNPERAIPENFFLYRFVTLLPDNFLQVRVVTKDGQRI
                     TGIRLNEDPFSIQIRDFSDHIYSFWKSELAELHKDWGKSPMPSYRGVFTPAEMDDLIA
                     YLVSLRGGR"
     gene            36494..37537
                     /locus_tag="VFA54_01140"
     CDS             36494..37537
                     /locus_tag="VFA54_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZS49437.1"
                     /translation="MDLREFEAKLERIRRWLAAERYDAVEITRYSWLAWLLEGAEARV
                     LLSSERGNCSVIVTAEKIFMVANNIEAPRLKEEEIGELPLEWRIYNWWERPPSVLPQN
                     ARVASEMPASLRFSLLEPEVARARKLGREAAEGLETAARSLCPGLSEREIAGRIAAQA
                     MGRGLTPAGLFVAADERGKQFRHPIPTAKIAYRYAILSLVARRHGLHASVTRTVSFGP
                     ACGEMLRRHRAASRVHAAMLVASLPGTPLGEIFAEAQRVYRDSGFAEEWTFHHQGGPA
                     GYESREARAAPGSAERLQENQMVAWNPTIRGAKSEETALAAGRRVELLTRTGCWPELE
                     GDMPLADVLMIES"
     gene            37597..38922
                     /locus_tag="VFA54_01145"
     CDS             37597..38922
                     /locus_tag="VFA54_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS49438.1"
                     /translation="MTPQKLQEAAPPVIKPWYTGLTGYHWWVLSVASLGWLFDTMDQR
                     IFVLSRGPALMSLLPAGTSPADITWYSGLATSIFMAGWAIGGFYLGIIGDRWGRARTM
                     MLTILMYSLFTGLSALSRNWWDFIAYRFLTGLGVGGEFAAGVSLVAEVMPDHARAHAL
                     GILQALSTVGNITGSAISLVVLPLGWRWMFLVGIVPALLVVFVFRTMKEPEAWQKARA
                     SAQVGAQNRMGSAGDLFRDRRWRRNTFIGLSLAISGVVGLWGIGFWTPELIRSALASA
                     PAASRNWYASMGTLLQDVGAFFGIYGFTIITARVGRRKAFVAAFLIGLAATVMTFGWL
                     RRPQDVFWMIPVLGFCNLSVFGGYSIYFPELYPTRLRSTGVGFCYNVGRIVAALGPFT
                     LGGLTVVFAKAGFASPFRAAAILLASIYLLGVFTMRYAPETMGKPLPEE"
     gene            39052..40557
                     /locus_tag="VFA54_01150"
     CDS             39052..40557
                     /locus_tag="VFA54_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49439.1"
                     /translation="MKYFIYKSLAEIEADAERIGAKHVAFEHNPAKVQAVLSQPAAVG
                     GFRVGNSIAIHPMEGCDGDLDGSPGELTWRRYERFGRSGAKLLWFEATAVREDGRANT
                     RQLWIHEGTVDKLRNLLGHTRAVHKELYGTADDLLEVLQLTHSGRHSVGRKMIAVHNP
                     CVDAKTGVPASYPVVPDEELERLEDDYVAAARLAWQAGFRAVDIKATHGYLVAELLGA
                     KRRPGRYGGPLENRLRLLRNVIGKIRSELGNQLLVCVRLGCFDGVPFYADAATNKGVP
                     CRYEVPYPHGFGVDPADPLREDLTEVKQAVRCLESWGVALLNISCGSPYFDPHVLRPC
                     EKPDDGNYDAPEHPLLGVDRHFRIAAELQQTFPRLPMVGSGYSWLQKYAINAGAKNIS
                     EGRIRFFGSGRGALAHPDFAREALERGELDERKVCKTLTFCTFLMRQKNHPMGQFPCG
                     CPPFDKQGYGRIMKLAVEAKRSQRAPNSLPAGGPETARGGNDPVANRDKTR"
     gene            40523..40903
                     /locus_tag="VFA54_01155"
     CDS             40523..40903
                     /locus_tag="VFA54_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZS49440.1"
                     /translation="MTLLQIETRHVEPDITILEFTGNITAAESRRVEVLVEDLLKQNY
                     RKLVFDLTEVNYIDSSGMGSIAHCFARAIRNDGGLRVAGVNSRVKHLFHLTRLDAVFP
                     FYATSLAACAGFEPAAKPGEQLLW"
     gene            40925..41317
                     /locus_tag="VFA54_01160"
     CDS             40925..41317
                     /locus_tag="VFA54_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZS49441.1"
                     /translation="MRAADLLYATAMLQIDTRELGSGITVVTLAGKITLGLESQQIES
                     VVKDLLKQNRRKLIFDLSGITYIDSTGLGILTFCSSRATEAGGKLCIAGAASLPARLF
                     HITRLDTIIALYPSVEAARESLAAGTSG"
     gene            complement(41335..42465)
                     /locus_tag="VFA54_01165"
     CDS             complement(41335..42465)
                     /locus_tag="VFA54_01165"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HZS49442.1"
                     /translation="MRFHPAIVLGCLCGIVLMAPMAWAPRHRILAGVNDFVMFYAGAK
                     LSGTPDLYNPARVRQVQGDSAGSTGENYLYCRPPYLALLIRPLGRMPYHTAYLLWQLL
                     LLAGFAGFLALWRPTPFPITVLFSCLSLPLFACFMNAQDLPLLLLWIALSLRLEREGR
                     HFWAGLIFSFCAAKFHLFLLVALLIVGQRLVRFAFGLAAGGLFFVTLSFATAGLGWPA
                     RYWEILKLTTTVDVVEQMPNLRGLFFGWPHSAWWEGLVGLTAVIAVWIIVRNADFEMG
                     LAAALAGGLLIGVHGSVADAVLLLPACLIVYARTAEGWPRALAIVLLMPVGYVLWASP
                     LPMLRVLVPLSILGMLYSTAFLARLGSLRPGSGTAFGWQTVA"
     gene            complement(42792..43793)
                     /locus_tag="VFA54_01170"
     CDS             complement(42792..43793)
                     /locus_tag="VFA54_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS49443.1"
                     /translation="MKQALSILTLETVVLAALLWFPARSAAQQPSKSEDLPDISVDVD
                     VVNIFFTVTDKQGRLITNLDKNDFTLEEEGQPQTIKYFSRQADLPLTMGLLVDTSVSQ
                     GNLIDLEREASSQFLNQMLRMKKDQAFLISFDVNVDLLQDYTDSVRLLRAGLDKLHVN
                     GGSGGLGGGPMPTSAKPKGTLLYDAVFLAADEMLKNQVGRKTLVLITDGNDQGSQMDL
                     KQAIAAAQRADAIIYSIEYLDRGFYYRAGGYGMIGGFGNGSALKKLSEETGGRVFEVS
                     KKKNLKMIYDEIQQELRSQYSIGYTPPRSGPGFRRIKIIPRNRDFKVQARQGYYAKN"
     gene            43815..44810
                     /locus_tag="VFA54_01175"
     CDS             43815..44810
                     /locus_tag="VFA54_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS49444.1"
                     /translation="MRFTLSSIVAVLGFVSLGGLAASQDARSGENAPPVTIPVTVEDS
                     KGDLISGLEAGDFRLFENGVEQKVTGFSADAQPLSIAVVIDAALSGPTSERLRATFPS
                     LLAALSESDEAGVFSFDVDFRTVAEFTSDKDKIEKALKQIPLGADYGIAGEPLGAPAP
                     VINGQQIGGAPSQYPGAGQRPRKDIDSALISALDALTQRPAARRRIVLLVTDGVNSPH
                     NPISSSKVLETLRKTDVTVYAIGLDSAKLPRGSGQLARFCEATGGELFKAVKKTSIEN
                     LYARITEQARYAYLLSYMPAGAAAGFRSIQVYVSKPHARIIAREGYLAGRRPSQP"
     gene            44807..45406
                     /locus_tag="VFA54_01180"
     CDS             44807..45406
                     /locus_tag="VFA54_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZS49445.1"
                     /translation="MKRAVVHLRAVDPVLRTIIDKVGPCRIQYREPEFETLARAVVYQ
                     QLHGAAAAAIFGRVLAMAGNRDGRLTARSLLRIPKKDLRAAGLSAPKITYLRDLAEKT
                     AAKIVRFDRFPNMEDAEIIASLTQVKGIGVWTAHMFLLFALRRPDVLPVGDYGVRTAV
                     RDAYKLKDLPTPAELEAIARPWRPYCSFASWYLWRSREL"
     gene            complement(45506..46651)
                     /locus_tag="VFA54_01185"
     CDS             complement(45506..46651)
                     /locus_tag="VFA54_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49446.1"
                     /translation="MHKIPKSTLPADARAGQGSGPRLREATFDDYAQIAALESRYGLE
                     AKTYRQWAHLWAGNPACRYLPHWKIGWVIEDNGRIVGSVGNIPLLYEFEGRRILAASG
                     RSQVADPAYRSASLLLLDRVINQPGIDLYLNNTMTLPAAAAFEVFACPKVPSGLWDQS
                     AFWITNQRGFIESVLCKRKYRMTRLLSYPASAASFLKDWVTQSPLQEGDTEIRAFPGF
                     DQRFEKFWMEFRANHPRVLLAVRTQEVLEWHFMHAVLDQRLWVAAAMHGPKIAAYAIF
                     DRQDNPEIGLKRVRLVDFVSLDGSTALLEPLLAWAVRKCRAEGIHMLESVGRWLERGE
                     FMDAAAPHRRKLSTWTFFYLAKDAALARALTDRRAWMPSLFDGNASL"
     gene            complement(46661..47941)
                     /locus_tag="VFA54_01190"
     CDS             complement(46661..47941)
                     /locus_tag="VFA54_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HZS49447.1"
                     /translation="MSGSTSPAMDGQDAALSLYQRLDPGVLANPYPLYHRLRREDPVH
                     WDPYLHAWVVTRYKDVTAVLQRFSAERMPTPAQLTSLGLAELNPVAEVLVRQMIFMDP
                     PNHTRLRALAAAAFTPARAEAIRLHIQEITDELIDRVIEKGRMDVIADFADLLPATVT
                     AELMGVPVSDRDQLKTWSKDFSEMLGNFQHNPGRAALMLATVEKMTAYFRERMREQVR
                     HPREGVVHALMSAEIESDRLSEEEIIANCIITMTGGQETTTNLIGNGLLALLQNPKEL
                     ARLQADPALLPAAVEELLRYESPIQHTARLAPADAELGGKMIRKRDAVIAVIGAANHD
                     PERFPDPDRLNITRTDNRHLAFGWAGHYCFGAPLARVEGQIAFSTVFRRLRNLRLDPV
                     PLAWRHNMVFRGLERLPIAFEAAPRAQQGLVKAA"
     gene            complement(47938..49812)
                     /locus_tag="VFA54_01195"
     CDS             complement(47938..49812)
                     /locus_tag="VFA54_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HZS49448.1"
                     /translation="MTTSPEKSALCTAAAPAAREARLVPEMVAGQAAERPDAIAVASG
                     AEVLTYLELHRRAGAFAAQLQALGVEPGDLVGLCLPRCLDMAVAALGIWRAGGAYVPM
                     DPGYPAERLAFMLEDAQAPVLVTNQHFIQRFAAWSGAVVDAASLLAGDGRADPLFSEI
                     MPHELAYVIYTSGSTGTPKGVEITHAGLSNLVTWHRRAFAVTAADRASHLAGLGFDAA
                     VWELWPYLASGASVHLADEAVRSSAELLQEWLLAKKVTISFIPTPIAEQIFMLDWPAS
                     VSLRILLTGGDTLHHYPPANLPFAVFNNYGPTECTVVATSTRLGPGEYPDTLPPIGRA
                     IDNTEIYLLDERALPVPAGFPGEICIGGPSLARGYRNRPELTKEKFVPNPFHPEPGSR
                     LFKTGDLASLLPDGQLAFLGRADDQIKIRGCRIEPKEVVSALNRRPEVRDSLVVARED
                     EGGEKRLIAYVILDETSRVTHTELRDFLRGQLPEYMLPALFVRLAAFPLTAHGKIDHS
                     ALPPPDSGNTLQDQASILPRTPTEVCVAGILAELLKLDAIGRNDNFFLLGGHSLLGAQ
                     MIAQLRGIFGVEISLRSLFDAPTVAALSAELDRLAEEKRIRSAGAPTGTPGCCAEAKS
                     "
     gene            complement(49817..50674)
                     /locus_tag="VFA54_01200"
     CDS             complement(49817..50674)
                     /locus_tag="VFA54_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZS49449.1"
                     /translation="MVLDSPRLSRRQRGGVIWAAPRGPLKLNVGEIHVWRANLDQHGG
                     SLGALARMLDVEERRKAALFPLLRHRRRYIIARAALREILGRYLGVPPPGVMFRYGER
                     GKPELGRGSLHFSVSHSGQVAVFAISGAGPVGIDVEQIRDGIDRTVARSFLSPEAARE
                     LDALAPHRRLQTFFKAWTRMEACTKACGWGIENGIRTLEKFLYPGPERLLSFTSAGSP
                     DYAPDRCVHHICVHDISAHKGCAAALAAGTTRRPFRYWTWEADQCRYGALRLFPDEAA
                     SHTSCELTR"
     gene            complement(50665..51957)
                     /locus_tag="VFA54_01205"
     CDS             complement(50665..51957)
                     /locus_tag="VFA54_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZS49450.1"
                     /translation="MNHLPGQMPMIITDPSTPVLILASPHHGGLGITRSFGRLGIPVF
                     NVDAARATPAFFSRYSRGKFIWDIDSTAPAKSVEFLMRIPKRAGRPYILIPTTDTATV
                     FVAENAEILRQSYLFPEVSPALIHSLCDKREVYHVAQRLNISIPRTEYPRTKADLLHY
                     FESCGRPVMIKGSGVLLRRQNSKTKFVIRSAKQLLDLCARMEEGAVKNVVVQEYISDG
                     EDWMFNGYFDEHSHCLAGFTGRKLRQCPAYTGVTSLGLTQKNETIARIAKRLVQSLGF
                     RGIVDIDFSYDARAGEFKLLDVNPRIGSTFRLFVSRDDLDVARALYLHLTGRPVPPQA
                     AAEGRKWIVEDLDLAASLRYALDGKLTLRQWVKSLSGIREAAFCAADDPAPVLLMLWA
                     DLRELWRWAQARRCPAPPFDYEPSPAPAPEYSEENVWS"
     gene            complement(52010..53185)
                     /locus_tag="VFA54_01210"
     CDS             complement(52010..53185)
                     /locus_tag="VFA54_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49451.1"
                     /translation="MALENGGPAAGNRSSSQVNSEALRVLPYTEDLVGAVTEFNERIR
                     RAGAPFLLPVRPAPDWLPKVGDRQIYEEIFVVVQDGFVRGAYTLKRQEFSFAGRLLPA
                     AACRMPISEAIADKRYGLVGPKIIHDALRRMPLLYCLGIGSLDANISRMLRAMGWTWR
                     VVPFFFKVLNGFRFLRNIEYLKTGLPRRLALDAAAYSGMGWLGARTVRLLLKAKTARQ
                     GLPLAEEVSEFASWTDEIWRMCRSRYSMTAVRDSEVLNILYPASDPRFIRLKICAASR
                     AIGWAVLLDTVMSCNKYFGNMRVGSIVDCLALPEDAPQVVAAAANNLEHRGVDLLVSN
                     QSHPAWCDGLKKAGFFVGPSNFFLLMSKQLSGLLSDLDPALSNMHLNRGDGDGPIHL"
     gene            53865..56663
                     /locus_tag="VFA54_01215"
     CDS             53865..56663
                     /locus_tag="VFA54_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HZS49452.1"
                     /translation="MKPSVSRVTGLAIAVPSLLFCWLLLPGLAGAQPVVWVASDMVRV
                     GASDAPGSGTQAQLWAGRGEYEAFQIIIRAPSGGLSNVNVTVSDLSGPGGQTIPRSSY
                     TLYREKYVYVSPGSPNWGGSNQPLGAGWYPDALIPFTDPATGAPLSGASLQAVPFNLA
                     GNTNQPIWVDLLVPRTAPAGQYNGTFTVTSSQGSVSGSVVLNVWNFTLPLTPSLHSSF
                     LYWTAGSLAAQEELLRHKISPLATSPSNQSLLMNSYGLGSTGLPFWSGADVSTCSMSP
                     APSVSQFQASASAQQPGLLLYDYSADEIGNCSNLITTIKQWAYNMHQAGVKNLITMSP
                     NPQLYDDGSGTGRSAVDIWTMLPIMYDQNVSAVNYVLQKGDAAWSYNALVQDSYSPKW
                     LIDFAPMNFRIQPGFISQALGLTGMLYWRIDDWSSDPWNQVNNTGTFSSNNYPGEGML
                     VYPGQQVGINGVVASMRLKYLREGVEDYEYVQMLKNLGQGTWAMNLVKTIASSWSNWT
                     RDPNALASVRMQLGQQLHSLSGGSPTSSGGGSSGGTTGGTAPAAPASPSPANGATGVS
                     TSPTLTWSPSSGAASYDVYFGTASNPPLAATVATASYAPGTLSSGTTYFWKVVAKNSV
                     GTAASSVWSFTTSAPVVSSSTPVAVSVTPSSGSGMSAVFSLVYSDASGAADIAGAGAL
                     INATFNGQAACWFYYDRASNQISLASDDTTTWTAMTLGSGNSLSNSQCSISGSGSSAV
                     ASGSNLTLNVAVTFQSAFAGSKTTYLYVQNKAGQSNGYQAKGTWTVSSAPVPPVAVSV
                     SPSSGSGFSSVFQFIYSDGNGASDIAGAGALINGSFNGQGACWFYYNRASNQISLASD
                     STASWSAITVGSGGVLKNSQCTIAGVSVSAAASNLTVTVPITFQANFAGSKAVYMYVQ
                     DKEGQSNNYQNKGNWIVP"
     gene            complement(56633..58348)
                     /locus_tag="VFA54_01220"
     CDS             complement(56633..58348)
                     /locus_tag="VFA54_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HZS49453.1"
                     /translation="MINYSLPLGRLILRLIALSIAVAVPEATEQTVSTLTVWVATGLD
                     RIGLADAPGPNASAALVSAKAEYEPFQVVVQAPPSGLTGVSVSISDLTTADGRVIASN
                     NATLYREHYVYVDRGSPNAKGSNQPLGPGWYPDALVPFLDPKTGSPPAGGAYPAAPVN
                     IDGGKNQPFWIDIYVPRDAVAGDYSGTVTVTADQGSAAIPCKLTVWNFELPAKPSLKS
                     SFALWSATTNEAREELIKHRIMPQRLGSCGANPCTGLAALEQTLIANFGLTSTDLGFW
                     SGADASHCNMRPPPSPLALQAAASAHAPDLNLFNYTADEIGACTNLYPMIRQWGNNLH
                     QAGVRNLITMAPDPALYDDGSGTGRSAVDIWVVLPKTYDSAPQRIAEALAKGDEVWSY
                     NALAQDSYSPKWLLDYDPINYRIQAGFISQSLGLTGLLYWRVDKWSSDPWAQVNNEGV
                     FTSSNYPGEGMLVYPGQPAGVSGVVPSIRLKQLREGSEDYEYVEILKRLGQGDWALQV
                     AKSVGPDWKNWGRDKNALEAARRQLGDKIQQLQAQQGAAAPARRGRASAGKRLAYGTI
                     QFPLF"
     gene            complement(58494..59648)
                     /locus_tag="VFA54_01225"
     CDS             complement(58494..59648)
                     /locus_tag="VFA54_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49454.1"
                     /translation="MPAPDENFHRSLKRAVAIISLDAEQIWGHLDLLNEQQFCERYPS
                     ALAAYDRLLGVLCSAGVSATWLVVGGMALAESDGPRDPRLLGLPARWTRSIPPGDEGI
                     AALWYRRSFVKRLAEARPKQDVGLHGGLTHLIWTDWRSKPEVIRREFFGGIEALEAVR
                     VRPVSFSFPRNQEACHGLLASNGILCYRGRPPVLSEKLGRTLPGSLLRILEELGRATP
                     PPVWPKETLPGLWNIPASLFLYPIGESRSRLVVLRSRIERVARGLDAAIRHNGVFHFC
                     LHPVNLAESPKGFSVFEGILERILKARESGDIETLTMAELAARMAQEKGNGISAPALY
                     TETLAPSPALSELCMNPSSGPEGAALTGKTGAPGPVNLHLEDEPRLQNLS"
     gene            complement(59667..61247)
                     /locus_tag="VFA54_01230"
     CDS             complement(59667..61247)
                     /locus_tag="VFA54_01230"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZS49455.1"
                     /translation="MSLGKISLACLLDFILVHVCMAMALAFAVTFHVILGDQQTAHNI
                     ATEFPQYYWHCFWPLSLIFPAVFMASGFYQKPGFYFIRYRRTAIVKGAGLAVLFYLLA
                     NYLVFQEGLVSESLAAWFAALVIIAVTLARAAQDAFRRTRRAVEKTAPRRLADPASVL
                     IVGGAGYIGSILTRRLLDRGYNVRLLDSLVYGYASIRDILNHPNLQLVVGDCRNIQTV
                     VGAVKGVHAIVHLAAIVGDPACEQDRQTALEVNYAATRMLIEVAKGYCIGRFIFASSC
                     SVYGISEELVDERATVNPVSLYARTKVNSEEALLEAHSESFHPVILRLSTVFGHSYRP
                     RFDLVVNLLTAKAYQEKVITIYNGQQWRPFLHVRDVAPAVEHVMTAPLGVVSGQIFNV
                     GDPRLNFTLSQVAEKIRHEFPGICVENIDNADHRNYRVSFTKIQNQLGFRSQWSLEDG
                     IRELKSALEAGEVTDYRDFYYSNQKFLEVAGVSAQADETDSRVMAAFAGSRTEAKRPF
                     LDVDGRFGPRGPAVSISR"
     gene            complement(61284..62477)
                     /locus_tag="VFA54_01235"
     CDS             complement(61284..62477)
                     /locus_tag="VFA54_01235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZS49456.1"
                     /translation="MRSAFLPYCQPFIGPEEIQEVVDSLQRGWVTTGPKVKKFEQDFA
                     QYVGVKHAIALSSCTAALHVALAAFGIGPGDEVIVPTLTFCATANVVAHLGATPVLAD
                     VDDNFHISLDAIRRSLSGRTRAIIPVHYAGQACDLDALLDLGKKHDVLILEDAAHAVG
                     AEYGGKKIGAHGQAVAFSFYATKNMTTGEGGMLTTNDDWLAGRIRLLSLHGMSRDAWK
                     RYTQPGSWYYEVHEAGYKYNMTDIQAALGIHQLQRLDGFIERRREIAESYAAGLADVP
                     GIRLPAEAPGRRHTYHLYPLRVDSMAGGRDLLIEKLRQANIGTSVHFTPLHRHPYYRR
                     ALHCSEASFPIAEKIYNELLSLPLYPKMSDSDVADVIVAVRRAISNGRSLRGLPPRGG
                     YAAAS"
     gene            complement(62523..63509)
                     /locus_tag="VFA54_01240"
     CDS             complement(62523..63509)
                     /locus_tag="VFA54_01240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS49457.1"
                     /translation="MSNSLAVLPESEVAVRFIVENLARRRVYQDLAPAEAFQRLSQKR
                     VERLLSSEQASLLLQWSGQSLCGAACWSKLLWDSELFGFPSARLEFLVAEGDYWDSRH
                     VKRRLIGDVYKQCQREGVRHLTARVDTGDFSAIHALEASGFELIDGIQTFAISLDGLD
                     LEAPPAATPSGVCVRLFRESDLDQVLKIARTAYIYDRFHADYALSSAAADHINEAWLR
                     NSCLGSAADAVIIASQGESVLGYVTCKIDQEAASTIGVSFGTIVMVATAENARGQGVA
                     SLATLGALRWFREKGVRIVEVGTQLRNLAAGRLYEKCGFRLCGASLTFRKLV"
     gene            complement(63502..65304)
                     /locus_tag="VFA54_01245"
     CDS             complement(63502..65304)
                     /locus_tag="VFA54_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZS49458.1"
                     /translation="MKVLHIVGESKFGGGSIIILQLARHAKSMGWDVDVLTTDATFQA
                     ALREAGIGVVALDVIWRSIRPVRDVVGLLRLYRFLKTARYTIVHTHTSKGGFVGRLAA
                     RLAGVPIIFHTAHGFAFHERSSRLVIAACSVLERAASRWCRRIFTVSEFHRRWGLRLR
                     IAGGHQIRAIPNGIPQSRVEAAAGRDAVRASLEAGAKEVVVLSTGRLAPLKGIEDLLR
                     AASVLISRGLPLSVCLAGSGPIREGIAALAVELGIAPRVRFLGFRNDVGDLLAASDLV
                     VLPSVREGLSIALLEAMAAGKPIVATAISSNCEATRNGSAALLVPPGDDGALAEAMQK
                     LIADPSLAERLGRTARSVYLASYTEQTMLEKYADEYCAAVRELETRTKNPALHKAKLY
                     PAAKRVLDVAISGLLLLLLSPLMFLIAAAIRLTSPGPALFRQRRLGRYGVPFTIYKFR
                     TMRAGCADLRNADGSTFNGDRDPRLTAVGRFLRKASLDELPQLINVLAGDLSLVGPRP
                     ELPDQFSFYTPAEKRRLDVKPGITGLAQLNGRNSIPWQQRKQLDVEYVEKRSMLLDVK
                     LLLKTAPYVVSRRGIYVSAQPESLAENQPVHSHE"
     gene            complement(65338..66699)
                     /locus_tag="VFA54_01250"
     CDS             complement(65338..66699)
                     /locus_tag="VFA54_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HZS49459.1"
                     /translation="MVKRFLSVRVPSGLAHNVAALYGLYCSGYLLALVTVPYLSRVLG
                     PSSWGEVAFVQAFGGYLLLIVEFGFTFSATREVARHQEAKDKLSELVAGVMGAKLLLV
                     ALCLVMALGVRHFVLPLRQHSALFWAGLFWSLVQGLNMMWFYQGIERMKAAVTIDLLA
                     KSSAVAAIFFLVRAPADGWKVLGIQGGASLLSLAGSLGLVYRDLRFRLPRISLLMGAL
                     RGSITTFVPRNASIMYTVGNAFILGLFAPPHVVGYYAGADRICRAILGLLGPVSDAAY
                     PRISNLAKRSRPETQRFARLAALVLVSIGLLMGLFMFAFAPQLVRILLGKGFEPATVV
                     LRVFAILPPIVALRNVLGVHWMLPLDLDRPFNLIVLGAGVLNIGLAVLLAPRHMDVGM
                     AWAATLSQLAAAAATYAILRWMRLDPLSRKPDRAGGDQKNVREAASSQEPQLEYLLDH
                     VVE"
     gene            complement(66693..67862)
                     /locus_tag="VFA54_01255"
     CDS             complement(66693..67862)
                     /locus_tag="VFA54_01255"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS49460.1"
                     /translation="MPLFLGAYVFLLPVQFESNTMNLAPSDLFLALCFAIELGRIRIV
                     RGAWSVFHAALVIIFFISTFVALNQLAKLTSYILIKDLGLLSLMGSYLCITSNCGSWS
                     AIRSLLRIFIVAAALHALLGVVVYFSGVSVPRINYGAARVSGMLIDPNAFGGLLVAAL
                     VPNTVTLFSPRPLLGRTPAALASVMLATALMLTFSRSAWIGLAAALLVIAAFRPSLLP
                     AYAAMGAAAAFAVMLLLGQSRAVFLARRPNTAHQRLQQIQDALPFFSQSPLFGIGLGQ
                     FDQFTKGDPTVLRRPYIIHNTTAWMMTEFGLAGAGIFLGLMLWFFTRGAAAYRSMGPD
                     ERPLIVGLLGAHAGMLGLSAGIEALYQRHWWLIMAMIGASSAIAKRTAARAGRAW"
     gene            complement(67965..70229)
                     /locus_tag="VFA54_01260"
     CDS             complement(67965..70229)
                     /locus_tag="VFA54_01260"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZS49461.1"
                     /translation="MNSSAVHDDSIGLSRPEASVWPLGAPSTASSDAGLDTLITYWRL
                     IRRKAPFIFLCALAAVLGAWGYTAGQTPMYQAQTSLEYQPLNEAVTPFREPAAPAAPP
                     DGAGDLAMQTEVEILQNESVLGRVADRLNLLNRPEFTAPGLVDRVRNAAGLPVAPISP
                     RERALKMVRMRVGVWNQARLIHITYDSPDARFAAQFANTLADEFIREHQQARSETARR
                     VGEWLSGQVAEIRTTLESLERQTQDYAQNAGLLFTGEQQQDNVAEARLRLLGNELAAA
                     QADRIMKQSQYQAAADVPPESLPQVVGDNALRDYEVKLTDLRRELESLESLLTPSHYK
                     VRTVQNQIAVLEAARDRELAATRKRIENEYQAALRREQLLSTRYADQSKLVSSLAAKS
                     IHYNTLKREVETARALHASMLQKAQELSLGAAVPASNIRVLNRALPPARPYKPNYLLN
                     LSVALFAGLFFGVGVVLLREHSDVTFKSPGQALSILQTPELAAIPKPQRGCLAGLRAC
                     LPANPHRNGQKVELAARDGAYPLLVESFHDAAASLCIRYEGAAHVITLTSPGPGEGKT
                     TVAANLAIALAQTNRRVLLVDGDIRRPRLHKIFNLAGRPGLSDLLCDSSVLDTRAVRY
                     AEMVRGIPNLYLLPSGPLTIAPETKEAAGISALLHSERLPELVAQLRQEFDMVIIDAP
                     PALHGPDARLLSRAADGVILVLRAKKTSREAAVATLQRLLADNLTVLGTILNGWDPRT
                     DSFYGYATYATRKL"
     gene            complement(70284..71318)
                     /locus_tag="VFA54_01265"
     CDS             complement(70284..71318)
                     /locus_tag="VFA54_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZS49462.1"
                     /translation="MPSKLFRDLWPLKAEGSIALMLAMMLLAAPSGRPQHQAAAGGLA
                     SPIDPGNAEAYMLGPGDELSVWALGSEEISAKPIRVDSEGYLDVPLIGRILALGRTPE
                     QIKAEIISRLRVFVKEPQVTVSVTDFHSQPVSVIGAVNKPGIQQLRGRKTLIEVLSMA
                     EGVRNDAGNTVRITRRSDRGQIPLRTAVQDPTKRFSMAEVSLRELMEGSNPSANILIE
                     PDDVISVSQAQTVYVIGDVHKSGGFALSEREHISVLQALSLAEGLGPNAAPEKAKILR
                     TSASDEKRVEIAVNIKQILAGKSRDIMMEAEDILFVPNNATRNVALRAAEAALNVGTG
                     ITIWRVGLPR"
     gene            complement(71323..71928)
                     /locus_tag="VFA54_01270"
     CDS             complement(71323..71928)
                     /locus_tag="VFA54_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HZS49463.1"
                     /translation="MFPGNSQRPLTPLMNRWRPEPETPSAGLRDDSEALEWFALAVRP
                     RHEKAVSRLLRYKGYETFVPLYRRQHRYGRRLRQFELALFSGYVFCRFDALVRLPILM
                     TPGVIRLVGAGRVPTPVDDNEIKALQSAARASFPMQPHAFLTAGERVRIASGPLAGVE
                     GVVVDSTRPFRLLLSITLLQRSVLVEINEDLVLRQREISWS"
     gene            73345..74727
                     /locus_tag="VFA54_01275"
     CDS             73345..74727
                     /locus_tag="VFA54_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS49464.1"
                     /translation="MNRRRVLVVDDDKSILAYLSDLLADRYDVDCLDSGEGVVPYLGS
                     GARPSLILLDLMMPAVSGLDVLAAIERLSRRPPVIVLSAIGQINTVVRAMKLGASDYL
                     VKPVEEQDLYQAIENALEESDDPEETAAPAAACGADIISANRQVLRVKQIAAQVADAE
                     VPVLLLGESGVGKDVFARYIHARSSRSAAPFVKVNCAALPADLLESELFGYDRGAFTG
                     ALREKPGKFELAGRGTLFLDEIGEMSPVLQAKLLHVLQDGEYSRLGSTRSTLSQARII
                     ASTNKRIEEAVSKGEFREDLYFRLNVIRLQIPPLRERREDILPLSHFFLEKYRPQYPR
                     SPRQLPRELIQAILLHSWPGNVRQLENTIKRFLVLPDLDFALAELRESGTPFNVSSPP
                     QNRSLKRVSALAAERAEKEVVLRTLDETNWNRKQAARELNICYKALLNKLRKWDIPGR
                     SKGPIEPSAL"
     gene            complement(74885..75487)
                     /locus_tag="VFA54_01280"
     CDS             complement(74885..75487)
                     /locus_tag="VFA54_01280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS49465.1"
                     /translation="MARAALRSRKIARLGRKPFLCILRALVDRLSFHCLKIGRLYLLK
                     LDHPPYIPSSMLRGPGEIRAGSLNDLNRLAMCGENKYQKFLERFQAGDHCVVAQLGGG
                     IIGYEWFSIRPRHVAEPFKYRITVPCDCIYAYDAFILPEYRGSGVWLRFKSHLAALME
                     DLGRRRVLTYVEQGNELSLASHFRLGFKPVKLVFVSNIWA"
     gene            complement(75497..76486)
                     /gene="nadE"
                     /locus_tag="VFA54_01285"
     CDS             complement(75497..76486)
                     /gene="nadE"
                     /locus_tag="VFA54_01285"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HZS49466.1"
                     /translation="MNISTRFSAELLKMDAGGAALRLESAIREIVRRDLRRKGAVVAL
                     SGGVDSSVVAALCARALGRDRVLGLFLPEADSSADSLRLGKLVADQLGIRSVVEDITP
                     ILAAAGCYRRRDEAIRSVVPEYGEGYKCKLVLPELLGGPRYAVYSIVVESPSHVQTKA
                     RLTAEAYSQIVAATNFKQRTRKMMEYYYADLLGYAVAGTPNRLEYDQGFFVKNGDGAA
                     DFKPIAHLYKSEVYQLAEYLGIPEEIRRRNPTTDTYPLHQSQEEFYFSLPYDKMDLCL
                     YGKNCGAPVEEVAAEAGLSPAQVQRVYELIDSKRNATRYLHRQPVLVAPVPEV"
     gene            complement(76483..78006)
                     /locus_tag="VFA54_01290"
     CDS             complement(76483..78006)
                     /locus_tag="VFA54_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZS49467.1"
                     /translation="MQVETFLERSAEKSPEKTALVCGSRRLAYIEIERQSNRLAHALI
                     RQGVRRGDRVAVQLENSPEAVVALFAILKAGAIFLVIHPSTKAEKLAYILNDCRACAL
                     ITDSARIAGLEQQLSGLSHLKSIVLADAEWDRIVGDSVSPDTPPEKQCIDIDLAALIY
                     TSGSTGRPKGVMLTHLNIVSAATSITTYLENTPDDVILNVLPLSFDYGLYQVLMAFQI
                     GGTLVLERSFTYPRAVLETMIREKVTGLPLVPTMAAILLQMDLSTYRFPALRYITNTA
                     AALPVHQIAQLRKIFPHVKIYSMYGLTECKRVSYLPPDQIDIRPKSVGKAIPNEEVYI
                     VDERGQRVGPGTVGELVIRGSNVMKGYWGLPEETEKVLKPGPMPGERVLYSGDLFQMD
                     EEGYLYFVGRKDDIIKTKGEKVSPREVEEVLYGLEGIAEAVVIGVPDPVLGEAVKAVV
                     TLRDGSAISEQEILRHCSRHLEDFMIPKFVEFRAALPKSANGKISRRELASSAGEQR"
     gene            complement(78017..78286)
                     /locus_tag="VFA54_01295"
     CDS             complement(78017..78286)
                     /locus_tag="VFA54_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZS49468.1"
                     /translation="METLNQTLNQELRSLVVENLLFGQDDGKFSDDDSFLEKGIIDST
                     SVLELVSLLERQYQIKIEDEDLVPDNLDSINNLSRFIQTKLQPAA"
     gene            complement(78273..80435)
                     /gene="asnB"
                     /locus_tag="VFA54_01300"
     CDS             complement(78273..80435)
                     /gene="asnB"
                     /locus_tag="VFA54_01300"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZS49469.1"
                     /translation="MRGRPQRAKPVIGLTSWSRRRAGWHGVCIAARSGVRARLDVRSY
                     TAPGWSGGTIPQPAEEHPLAGETQPGWKRACEPMCGIAGVLNFYERADPALLRTMIGL
                     IRHRGPDATAVYCESHAGLAHARLSIIDIAGGCQPMQTEDGSLSITFNGEIFNYLELR
                     AELLEQGHRFRTQSDTEVILHLYRQYGEDCVSRMNGQWAFAIWDARRQRLFLSRDRLG
                     IRPLFYTVTGRAFVFGSEIKAVLGHPGVSRALDLDALDDIFTFWCRVPPRTIFQNVQE
                     LPPGHSMAVDGRDVRVWPYWRLEFNCAPHPERDSAERLFALLVDATRIRLRSDVPVGA
                     YLSGGLDSTITTALIRKFSDSRLKTFSVSFDDPGFDESAYQREAVRFLETDHEEVRCT
                     GREIAAVFPEVVWHVEQPVLRTAPAPMYLLSRLAASNGYKVVLTGEGSDEMLGGYDIF
                     KEAKIRRFWAAHPDSRIRPLLLRRLYPYLPELQRQSNAYLRAFFRIRPEELQKPFFSH
                     APRWELTAGLKLFFSDQVKSGLATRDCYAALEALLPPAYFEWDGFSQSQYLEASYLLP
                     GYILSSQGDRVAMAHGVECRMPFLDYRVVEFAAALPPPLKMRGLNEKYLLKECVNGLI
                     PKSIKQRAKQPYRAPEARSFFEGAPYDYVEELLSPGRLKQDGIFNPAPVQGLINRFRW
                     GGSGSIRDNMALIGILSTQLVIDQFIRSFSKRSGYGNT"
     gene            complement(80753..>83610)
                     /locus_tag="VFA54_01305"
     CDS             complement(80753..>83610)
                     /locus_tag="VFA54_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS49470.1"
                     /translation="TLEFDLSRSLDGAAVDAQAAITQAARLLPPGMPAPPTFTKVNPA
                     DQPILYIALTSGALPLWTLDEYAETRIAQRISMVSGVAQVQVLGAQKYAVHVQIDPHA
                     LAARQIGINEVEAALKNWNVNLPTGAIIGPQRAFTLQATGQLTSAAAYRPLVVAYRKG
                     TPVRLEELGRVIDSVEDDKSASWFSTHEGTQRSIVLAIQRQPGTNTISVTDGIKALLP
                     IFQSELPPSVKMNILYDRSDTIRESYKDVQFTMALTLGLVVMVIFLFLRNISATVIPS
                     LALPFSIIGTFAVMYLLNYSLDNLSMMALILSIGFVVDDAIVMLENIVRHMEMGEDPM
                     LASVRGSREIGFTIVSMTFSLAAVFIPVLFLGGVLGRLFREFAVTICVAILISGLVSV
                     TLTPMLCSRFLRPPHEQQRSWFYRVTERFFEGMLRVYDVTLQSVLRHRPATLTTFVIV
                     LVATGYMFLKIPKGFIPDQDTDQLVIVTGAAQGTSYYQMVEYQKAIAEVVREDPNVES
                     LVSSVGGTTASTLGGPNFGELVVHLKPRSQRKLLVNDVIEELRPKLSAFPGMQVFLQN
                     PPTVQLGGQVTQSPYQLSLHAPDKAELYSAAEKLEREIAQLPELQDVTSNLQIRTPQV
                     NVSIDRDRAAALQVSAEQIENALYDAYGPRWVSTIYAAINEYKVLLELKPIYQADPNA
                     LSLLYFKSTGGQLIPLDTVARVNRESGPQTINHFGQLPAVTISFNLRPGVSLGDAVSH
                     IQELAEHSLPATVSTSFQGAAKAFQSSLTNLWVLLIVAIAVVYIVLGILYESYVHPLT
                     ILSGLPSAGFGALLTLFLFRMDLNIYAFVGLIMLIGIVEKNAIMQIDFALEAERNEGL
                     KPLDAIYEGCLIRFRPIMMTTMAALLGTVPIALGYGAGGEARQPLGLAVVGGLLFSQL
                     VTLYLTPVIYTYMSSAVERYRARRQRKIAPAATPTPA"
BASE COUNT        17097 a        25370 c        24861 g        16282 t
ORIGIN      
        1 gccgataaac agccccggct atggcgccgg aatacatata gccggcatgc ggggagacgc
       61 agccgatcgg agcggcgatt tctttcgatg gcgcgccggc cagcagatgt tgcagttctg
      121 cgcgcagcgc cgacggatca ccgggataaa attggccggc ccaggctgca cggcggagca
      181 tagcagctct cctgcggttt cgagcgaggt gaagcgaaat atgccaggcg gtaggatgcc
      241 cggcaggtca ttcggcgacg attttcatcg cctttaaaaa ttcctgatcc tgccgcgtca
      301 gtgtgattgc cacagtctgg ggggcggagg tctcctcttc ttccctctgg acctcttctg
      361 cactggagcg cttgttgaat ccgatagtgg cctcgagcac caaaaaaacg tcgtagccgg
      421 cttgtttaat ctcgccgatg gcttgggcta tcgcttccga atcggaaagc gactcgttga
      481 tggcattgcc cagctcttgc atgagttgct tcagcctttc atccatggct tcacgctcct
      541 cccggtcctt cctccgccac ccaacccgat ggacgcgctc catcggctgg aagttgcatc
      601 cgttccgcct ttccaaagcg tattgaaaca gattccaggt ggaaaggcaa cggccccacg
      661 tgcaacatat cgtcgccgcc gcctcattcc atagggtttt tgtcgtagcg cgcgtgctag
      721 gatgaacgtg gatgctcgat ttaagggcgg cggcagccac cacgcgcagc ctggcggagg
      781 ttgtgcgcca gatctggaaa gccctgcgca tccttttcca ggaagttctg ggcttcacat
      841 ttttcgtgct cggcgggtgg ggcgtcctgt ggctcgttcg cagctatcac cggtttcagg
      901 gcgatggcga atcggtgttc aaaatggccg tggtggcgct gtttgtcctc atgatgggcg
      961 cctgcggctt ttcttccttc cggcgcgcgc gccgcatcgg gcgcggcaag taagtacgtt
     1021 tgatggagca gcatcgaaaa gcgcgattca catcttccgg gattgaaatc cccatctgtt
     1081 atgaccctcg ggcgattgat tacgacgctc agttgggcaa tcctggcgaa ttccccttta
     1141 cgcggggcgt acagccggat atgtaccgcg gccggctgtg gaccatgcgg cagtacgccg
     1201 gctacggctc ggctcgggaa tcgaacgccc gcttccggta tttgatcgcg cagggcgcaa
     1261 aaggcctctc cgtggccttt gatctgccga cccaaatggg tctcgacgcc gatcatgcgc
     1321 tggccctcgg cgaggtgggc agagcgggcg tatccatctg ttccctcgcc gatatgcagg
     1381 agctcttcca ggacataccg ctggagcgcg tctcgacgtc catgaccatc aattccacag
     1441 ccgccatttt gctggcgctc tatgtggccg tggcgcggca gcagggctgc gatctgcgca
     1501 agctggccgg cacggtgcag aacgacatct tgaaagaata tgccgctcgc ggcacctaca
     1561 tttacccggc acgcgcctcc atgcggctcg tcacggacct tttcgcgtgg tgccaccggg
     1621 agatgccgga ttggaatcca atttcgatca gcggatacca cttccgggaa gccggagcaa
     1681 ccgccgtgca ggaggtggct ttcacgctgg ctaatgctgt tgcctatatc gaggccgcgg
     1741 tgggcgccgg cctggagata gactcgttcg ctccccggct cagcttcttt ttcaacgccc
     1801 acagccattt tctggaagag atcgccaaat tccgcgccgc ccgcaggctg tacgcgcgcc
     1861 tgatgaagga gcggttcagg gcgcggaatc cgcgttcctg gatgctgcgc tttcacgcgc
     1921 aaacggcggg ctcgacgctg acagcccaac agcccgacgt aaacatcatt cgcacggcga
     1981 ttgaggcgct ggcagccgtg ctcggcggag cgcagtcgtt gcacaccaat gggtttgatg
     2041 aggccctggc gttgcccact gaggaatccg cccggctggc gctgcgcacg cagcaggtga
     2101 ttgctcacga gagcggcgtg gccaacaccg tggatcccgc tggcggagcc tacgcgatcg
     2161 aagctttgac ggacgagatc gaggcccggg cttcggagct gatgcgccgt atcgatgcca
     2221 tgggcggcat ggtgagcgcg attgaacagg gttttgtgca gcgcgaaatt caaaacgccg
     2281 cttaccaata tcagaaacag gtggaatcag gcgaacgcgt ggtggtgggc gtaaaccggt
     2341 atatgccgga aaaggagacc ccgattccca ttctgcgcat cgatccggcg atcgagaggg
     2401 ctcaagtgga tcgtgtgagg cagctccgcg cagcccgcga caacgcccga gcggataagg
     2461 cgctcgacga ggtggagcgc accgcaagct ccaatgacaa cctgctgccg gcgattctcg
     2521 ccgccgtcga aagctacgcg actttagggg aaatctcgga ccgtatgcgg cgggccttcg
     2581 gagaatatcg ggaaaccgtg gtctggtagc cggcggcccg cgaacagcgg ttcgggaatc
     2641 agataattcg ttccgcgcgg gttcaaccag cggcaaatcg gggagaaaaa tccgtacgcc
     2701 ccagcctcgc ccggcgcatg ggcaggtgca gggccgagcg gaggatgccg acggagcgcg
     2761 gaatacttac ccaaaggtcc gcgtgccggt tttcttgcgc ggtccggcgt tacgcatagg
     2821 actgtggcca agcgcggaat cggcaatctc tttctgtaac agcgcgcgcc gctcctccat
     2881 ggtcggttcc cgcgcgggaa aatccttttc cagcttggta cgaatctgct ggaaagcctg
     2941 gacggccatt ttttccgacc ggaaggtttg cacctcctcg ccgaagcgca tttcgtacga
     3001 cccgtccggc ttggattcca gaaacagctt gcccggaaaa aaggacaatt tcatggcatt
     3061 tatctccgcg agcaggtcaa ggccttaccc gcagttcatt gatgaccttc ttcactccat
     3121 cgaccttttg cgcaacgcgc tcggcccgct gccggagctt ctccgagtcg atcacgcccc
     3181 gaagcgtcac cacccgatct ttgaccgaga cctcgataga attggttttc aagtcgcggt
     3241 cattcaccag agcgcgaatg actcgatcgt acagcacgtc gtccgttaac ggctgcttcg
     3301 ccgcagccag aacggcatgg caccataacg gcaaggtcag caggagggcg atcgttctca
     3361 tatttgcttg ccggcccgcg attcgagctg ccggcgccaa tccagcgctt ccacaaacga
     3421 ttcactctcg cgccagtcgc gccgcacttt gacgtgcagt tccaaaaaga ccttgcgccc
     3481 aaacagcatt tccatttcct ggcgagcctc ggtgcctatg tgtttcagcc gttcgccttt
     3541 cgagccaatg ataatccctt tttgaccttc ccgctcaacg taaatggtgg ctgcaatccg
     3601 cagcagtttc tcgctttcct ggaaatcctc tacaaccacc gcggtggcgt ggggaacttc
     3661 ctggcgggta tgccagatca ccttttcgcg cacaagttcg gcggccaaat ggcgcacagg
     3721 ctggtcggtc aggtgatctg gggggaagta ctgcgggcct tcgggcaaac gggcgatcag
     3781 ggcgtccagc agttccggga cgccctcgcc cgttaaagct gaaaggggaa ggtactcctc
     3841 aaaagcgaag gattgtttat accgctcaat gagaggaagc agcaaactct tatccgccag
     3901 acgatcgatt ttgttcaaag ccagcaaagc gggcgtgccg gcggaacgga tcagctccag
     3961 cgcttgttca tcgccgggtc cgaaagcaac cgtagcgtcc accaccagca gcagcagatc
     4021 gcgcgcttcc agggcggcgc gaacctcccg catcatccgt cggctcaagg tgttgcgggg
     4081 ctcgtgaatt cccgggctgt cgaggaaaat aatctgcgct tcgggccgag taaccacgcc
     4141 ctgaatggtc tctcgggtgg tctgaggctt ctcggcgacg attgcgattt ttgcgccgac
     4201 cagccggttg agaagcgtgg atttcccggc attcggacgg cccagaatgg atacaaatcc
     4261 tgatttcatg ccgtataggg aagctcggcg gaagattcct cttgtccggt gcggcggagg
     4321 cgcagttttt cgacccgcag cccgttgcta gcgagcactt cgatctggat gccgttgagc
     4381 tggagcttct ctccgggcgc cggcacacgg cccatgcgct cggtgaccag accggcgatc
     4441 gtggttgact ccagatcctg ctccggacgg tacccggtca ggtctttcag acggtccacg
     4501 tcgagatcgc cggagacaac caagctgtgg tcgggttcct cgattacatc ggagacaggc
     4561 tccgactcat cgcggatctc gccgacaatt tcctcgacca aatcttccat cgtggccagc
     4621 ccggcggtat tgccgtattc gtcgatcaca atcgccatct gcaagctctt ttcctgcatc
     4681 tcgcgcagaa gatcgttcac ccgcttggtt tcgggcacga agagcacagg ccgcagcagc
     4741 tcgcccacgc ggcgcgactg ccgctcactc tcctcgacct ccagcatatc ccgcacatgc
     4801 acgtggccct cgatcgcgtc gatggtgcct ttgtagaccg gtatgcgcga gtatcgctca
     4861 ttgatgagca actggcgcaa ctcctccaga gaggcagagg cctcgatggc tacgatacgc
     4921 gggcgcggcg tcattacctc acgtaccgtc ttgtccccga attcgaccac cgattggatc
     4981 aatttgcgat catcttcgcc caacaggccc tcttcctggc cggcgtccat tagcgcttca
     5041 atgttttcct caggagtggc cggcgcctcc tcggccttcg attccacgcc gagatcggca
     5101 atggagtagc cgaacccgac caccagcagg aggggccgca cagccagaga aagcgcccgg
     5161 gcgacggcca gaaaacgcag cgcccaccgg ccggtggtcc gtgtaaccag aatgtggggc
     5221 gctatgtaag cgaacaggat caataaaacg ccggcgagca ccagagcttc taataatctc
     5281 gcgccggtaa agccgcctct gccacgcaga taggccaagt cgaacgcgag gagcaggaga
     5341 atgcattgct tcaccgcggc gaaccgctgc atgccggact cggcctccaa cttgagtttc
     5401 gggcgcaaag cgtcttcaaa gtacgggagc gaccgggccg cctcgcgggg catgatgcgc
     5461 agagattccg aatagagcag atggaagtat gacgccagca cgagggcggc gcaaagcgcg
     5521 atcacaacca gaatggcaaa ggcaggagtc atcgtttcgg cggtccggtg tgtttgtact
     5581 tccgaggccg ctttttcagg ctattaagcg tacccgacgg ccgggcgcgc gccggtttca
     5641 ttcgggcctc tcgagaactc ccctcgtctg gatcgtggcg gctgccagcc cgttcgatga
     5701 ggccggaggg caagcggagg cgccggcgcc aatgtcgctc gtgcctctcc atgcgacccc
     5761 cgtcgcgttc gtgatcgtag cccaacaaat gaagcaggcc atgcaacaga agcactttca
     5821 gctcatcctc tatcgagtgt ccgagagcga gagcctgccg ccgggcttgg gcggcggaaa
     5881 tcagaatatc ccccaggtac atgccttcgc cgtcgggaaa cgaaagcacg tccgtcgttt
     5941 cgttctctcc ccgaaaccgc aaattggccc gccgcacggc ggcgtccgag gcgatcgtca
     6001 gcccaaactc ccggcccccg gcgaggcggc ggcgcaattc actggcgaag cggatcaaat
     6061 cggaacggtt gaagccgacg gctgtttgcc ggttggaaaa ccaatccgcc ggatcagaat
     6121 cgccggaact gtgttccgaa ggaggcataa ggcagctcag cttgcctgtt cggccgcggg
     6181 ggaagaagga ggatcggcgg cggcatcgct ctccgtttgc ggcgttccgc ccggcggcgc
     6241 cggatgatcg agcggaaaac tcaactggcg cccagccgcg tctccatact gctcatatgc
     6301 cttgataata cgctgcacga gcgcgtggcg aaccacgtct cgttcgtcga gataaacgaa
     6361 gctgacgccc tgcacgccgg aaatgacctg aatcgcctcc agcaggccgc tgcgcttgcc
     6421 ggcggggagg tcgatctggg taacgtcgcc ggtgataacc gccttggagc caaagcccag
     6481 gcgggtgagg aacatcttca tctgctcggg agtcgagttt tgggcttcat ccagaatgat
     6541 gaagctctcg ttcagagagc ggccgcgcat gaacgccagc ggagccactt cgatcagacc
     6601 tctttcgagg tacttctcta ccttctccac ttccagcatg tcgaacagag cgtcgtaaag
     6661 cgggcgcaga tacggatcca ctttttcctg aatcgtgccg ggcagaaagc cgaggcgttc
     6721 gcccgcctcc acagcgggcc gcgccagaat gatccgtgat acgcgcttgc tcagcagcgc
     6781 cgaaacggcc atcgccacgg ccaaatatgt ctttccagta cctgccggcc cgatcgcgaa
     6841 caccatgtcg ttgcgctcga tcgcctcgac atagcgcatc tggttgacgc tgcgcggcgc
     6901 cagggacttt ttgccgaagt tccgctgctt tgcgctggct accaggttgc ggagactcac
     6961 ctgagggtct tccaccgcca cccgcaggta gctcttcatg tctccgttgg taaacacctg
     7021 gccttgttgc accaagtaaa cgtagtcctc gaggatcgac tcggcgcgcc gcacgcgttc
     7081 gggttcgcct tcaatttcca aactgtcttc caggagatgc gcgctgacgc tgagagccga
     7141 ttccagcaag cggatatttt cgtcgcgcgt accaaagagc ggctcaatgc cgcgggtgat
     7201 tttgacctgc cgtttcattg atgcgggcgt ttaagcgccc taaaaaaaca aaaaacaggc
     7261 ccaagcctgt tcttttgcct ccttattact ctgtctgatg gctgcgaata agcccttcca
     7321 gttacggcca gcgccgtact ttcactatat cgaacctgcc gttccggagt caatcggccc
     7381 gggcgtccgt atcccggcga gaagtggcgg taaacacaat cattgcaagc ggtatgcgct
     7441 agggagattc ccaggccggc gtcaagccgg gcgcctgcca gcagcggacg cttccctcgg
     7501 ctcgatgtta cacaggggcg actttgggat catagaggcg cgggatgttt cgtgctggag
     7561 ccggcatcac gagctggccc ccgagccggg gcgagggatc tccctttcgc cgcgcggcaa
     7621 caggaatcca aaagcagatt ctccgcttag agcgatagag cggcgccctg ctcagtcaca
     7681 atggggcgac cgaccccgaa tcgcagtgtc gcgggttaga acatgaattt ggaccaagac
     7741 ggcaacagtc cggactgaac gctggccgcg cgcctggtaa ggtctcttcg cggattcccc
     7801 agccggcgcc cgcgctgcta ccctcgtccc acaaaaggca tcttggtggc catgatcgtc
     7861 atgaactgga cattggcgtc cagcgggagg ctggccatga aaacaatggc ctcggcgaca
     7921 tggcgcacat ccatacgcgg ctcgacgtga atgctgccgt tcgcctgagg cacgcccttt
     7981 gtcatccgct cggtcatttc ggtggccgcg ttgccgatat cgatctggcc acaggcgatg
     8041 tcatatttgc ggccgtcgag tgaaatgcac tttgtaagcc cggtgatggc gtgttttgtc
     8101 gccgtgtagg gagcggagtc gggacgcggc gcgtgcgcgg agatcgatcc attgttgatg
     8161 atcctgccgc cgcgcggatt ttgcgctttc atcatcttga tcgcttcttg cgcgcagagg
     8221 aacgcgccgg tcagattcac ccccacgacg gcgctccact gctcgtaagt aagatcttcc
     8281 atgggaatcc ccggggcgtt ggctccggcg ttgttgaaaa gcaaatcaag ccggccgaac
     8341 gcttccctcg tcttggcgaa cagagcgcgg acggcttcgg ggtcgttcac atcagtcgga
     8401 acgaccagca ttcttccgcc cgaaggcgtc gccatggctg ccgtcttctc cagcgcctca
     8461 gctcgccggc cggcgagaac gaccgagtac cctgccgatt gcagcgcgac ggcggcggcc
     8521 cgtcctatgc cgcttcctgc gccggtcaca actgcggttt tgccgctgcc tgccatgttc
     8581 gttgatcctc tccgattcgg atttgcccgc ctaaagcaac cattctgcct gagatcgtct
     8641 gatggtcaaa tggaaattgg tctttgccgg ggttgggccg ccgggcgccg cgattgcccg
     8701 tcgaacggtc agaatccttc ggcgcgggcg caacggcgaa cgttttcatc gtgcgcaaca
     8761 cgaggttcgg atggctacgg cattccagga gcaacacata atcgaattcg accggcacgc
     8821 gtgcggcgat ctggagttcg cactgcaacg cgagtggctg gagacgaacg gcctgggcgg
     8881 atttgcatcc tcaaccatcg ccggctgcaa cacccggcgg taccacgggc tcctcatcgc
     8941 ctccatcaca cgacctgccg ggcgatttgt cctgctgtcc aagctggagg agacgctgat
     9001 ccttgacggc cgccggttcg agctttccgt gaatcgctac cggccgggag tcatccaccc
     9061 gcgggggcat cggtatttaa ggcgttttcg tctggatccg tttccgatat tcacatatgg
     9121 cgccgagggc gtggagatcg aaaaatcgtt attcatgata catggcgaga acagcgtgat
     9181 agtgcaatac cagatgagcc aggctgaggg cgcgccggag catcacgagt gcttgttgga
     9241 attaaggccg ttgatcgcct ttcgcgacta ccacggcctc acttacgaaa acggcgtgct
     9301 cgacccgcaa gtcgggatgg aacccggaat tgcctgtgta agaccatatc cgggcctgcc
     9361 ggcgctctat atttcgcacc aggccgacgt ggtcgatccc gccggctatt ggtaccgcag
     9421 ctttgagtac gaacgggagc gggagcgagg cttggactac tgggaggatc tcttcagccc
     9481 gttcgcggcc cgcgtggatt tgaagcgcca acctgcaccg gctctcatcg cgtcgctgga
     9541 acgaagagcg gcggccaccg cccaggcctt gcggcagtcc gaaatcgagc ggaggcgggg
     9601 gattgcgaac gcttcccgct gcgagcaacc gtttgttcgc gcgctggcag ccgccgctga
     9661 ccaattcatc gccgcacgcg gagatcggaa gaccatcatc gccggctatc actggttcag
     9721 cgactggggg cgcgacacac tgatttcact ccccgggctt actctcgtca cgggccgccc
     9781 cgaaatcgcg agagacgttc tgatcggaat ggcgcgttat gcggatcgcg gcatgctgcc
     9841 aaaccggttt cctgaaacgg gcgccgcgcc cgaatacaac actgccgacg catcgctctg
     9901 gtttttcgaa gccgtccggg cctttctcga ttcccaggaa gattacgatt tcgtcgaggc
     9961 cggcctttat aaggcgctga cggacatcat tgcctggcac gtacgaggca cccgctacgg
    10021 tattcatgtg gattccgacg gcttgcttgc ttccggggct ccgggcgtgc aattgacgtg
    10081 gatggacgcc aaggtgggcg actgggtcgt tacgccgcgc tgtggaaagc cggtcgaaat
    10141 ccaggccctt tggtacaacg cgctgcggat catggaagat ctggcgcgcc ggttccgcga
    10201 ccacgccggc gcggcggagt acgcccgcat ggccgatctg gcgaaccgca gtttcaacgc
    10261 gcaattctgg aatgaagcgg aaggctgctt ttacgacgta atcaatggcg ccgagcgcga
    10321 cggttcgatg cgcccgaatc aagtgatcgc tctgagcctt ccgcatggta tggcgccccg
    10381 ggagcgctgt gtgcgggccc tcgacgcaat cgagcgcgat ctgctgacgc cttacggcct
    10441 tcgaaccctt tcgccgcgcg atcctcgata tcgcggccgt tatgaaggcg gcccttcgag
    10501 ccgcgacgcc gcgtatcacc agggcacggt gtggccctgg ctgatgggcc cttttctgac
    10561 cgcctacata aaggcgcacg gccgaacttc cgaagttctc gggcgtgcag ccgcgtggct
    10621 gcaacctttt gcgcagcacc tgagcgaagc cgggctaggg caaatatcgg aaatattcga
    10681 aggcgacttt ccgcatcgtc cctgcggctg catcgcccag gcctggagcg tcgcggagct
    10741 gctgcgcgcg gcggcgcaag atgtgttcgg agtttctcca gcgcaggcgg ctccgcgcga
    10801 aaattgagaa ggccgggcgc ctggtgcggc tccggacgcc gatcactcac gaaattcacg
    10861 ccagccggtt tatctgaggc ccctgtgccg cgcggccagc atctatcatg aaggaaggct
    10921 tccttacatg gcgccccaga tcatcgagca gtcctggcag atgcggctga gaagctgggc
    10981 ggatgtccag cgcgtccggc agcacgaaaa caagctgctc ctgattctga ctctcgttat
    11041 cggcgcagtg gtcggtctgg tggtcgtcgc attcatttgg cttaccgaga atctgggagc
    11101 gcgtatgtat cccgcgggcg gcgccgcctg gcggcgggtc tttgtaccga ccgccggctc
    11161 tttgatcgcg ggatttctgc tcgaccgcta ttttcctaac gccagaggca gcggtattcc
    11221 tcagacaaag gcggcgctgt tcctgcggga tggttacatt cgtttccgga cggttctcgg
    11281 gaaattcgcc tgctcttcgc tatcgctcgc cagcggcatt gcgcttgggc gggaagggcc
    11341 gtcggtgcaa gttggcgcag gcatcgcttc cgtactcggg cgccgtctgg gattaagctc
    11401 tgacaaaatc aggagcctgg ttcctgtcgg aacctccgcg gcactggccg ctgccttcaa
    11461 cacgccgata tccgccgtgc ttttcaccct cgaagaaatc ctgggcgatc tgcatgctcc
    11521 cttgcttggc tccgtcgtgc tgagctcggc caccgcctgg atcgtgctcc ggctcgtgct
    11581 cggggacgag ccattattcc atgttcccgc ttaccagttg gttcaccctc tggagtttgg
    11641 gatttacgct gcgctgggcg tcgccggcgg ctttgtatcg gtgggcttcg taaagctgct
    11701 gttgtttctc cgcaagcgct tcctggcgat gccccggcgc accagccctt ttcagcctgc
    11761 cgccggggga gttctggcgg gcttgctcgg ctggtttgcg ccggaagtgc tgggagtcgg
    11821 ttacggacac gtcagcagcg cgctgaatgg ccagatgagc ttggggctga tggcgttgct
    11881 ggcgccactg aaagttctgg cgaccgccgc ttgttatgcg tccggaaacg cgggcggcat
    11941 ttttgggccc agcttgttta ttggcgccat gacgggcggc gccgtgggaa ccgccgctca
    12001 ctacttcctc cctgactaca cgggcagcgt gggggcttat gcgctggtag gcatgggaac
    12061 ggctttcgcc ggcatcatcc gcgccccgct cacctctgtg atcatgattt tcgaaatcac
    12121 gcgcgactac tccattatcg tgccggtgat gctttccaac ttgatcagct ttttcatctc
    12181 ccaccgcttg caggaagagc cgatttatga ggcattgcag catcaagacg ggatccactt
    12241 gcctgcggga ggcggccggc atgaggggat tctgctggta cgggacgccg tccggcagcc
    12301 ggtcaaggcc ctttccgccg aggaccggct gagcgaccga atcgagatta ttgaaagccg
    12361 ggaagccgat gcctggccgg ttgtgaacga gagaggcctg atgggcatgg tgaccgcggc
    12421 ccaggtccgg caggccattg agcaaggtaa cggcgcccgg actttcggcg accttgtgcc
    12481 ggatccggag gcgatgcggc ccctgcgggc ggaaaacttt cctcacctgc atgccgacca
    12541 atcgctcgat ctggcgctgc ggcgcatggc ggaatcgggc ctgacggttt tgcccgtcgt
    12601 gagcagaacg aatttacgcg agctgcaagg gaccgtttct ctgaatgacg cccttaccgc
    12661 ctacgggttg ggcaaagatc gcaagccacc ggcgcaaccc gccgtcaaga gtcccgagcc
    12721 ctctgtgccg ctggcgagct ttctcgccgt gctggtggga attttagtgc tggccagctt
    12781 ctttacctat ctctaccgcg ctcaacgccg tgagagggcc gcgagagaat ttcagcaagg
    12841 aactgagttg gctcgacagg ggcgcgatca ggaggctatc gaaaactacc ggcatgcgct
    12901 ctcggtttcc cacagccggg agcaccggct cgcgttggct ttggaattgg tgaaggttgg
    12961 ccgctggaac gaagcccgtt cttaccttca ggaactgctc cgggaggacc ccgcaagcgg
    13021 cgtcgtccac ctggcgatgg ctagaattga ggctggagaa ggcagaaccc aggaagctgt
    13081 gaccgagtac aaccgcgcca tctacggctc gtgggccgcg gctgtggaag gccccgtgca
    13141 agcccgcttc gagctcgcgg atctgctggc aaggaccggc gcgaaacagc aagcagccgc
    13201 cgaactgata aagctgctcg gcgagacacc ggaaagcgat tttgcagccc gaaaaaaaat
    13261 cgcgcgcctt ttgctggact acggctccgc caggcaatcg gcggctatcc tgcgcgaggt
    13321 cttgagccgg gattctcaag acccggaggc ctacgcagaa ctgggagcgg cggagtttgc
    13381 tttggaggat tacgcggcgg caaaaagcgc ctacggtcac gccgcgcgat tgaatccctc
    13441 ggatgcggcg gcgcaagcgc gtctgggttt gtgtaatgag attctcgctc ttaacccgcg
    13501 tgctcgcagc ttgcgggcgg ccgaacggta cgcgagaagc aagaacctcg tgcagagggt
    13561 tcttcaggcg gccggtccgt gcattgcgag caggggcaat gttccacttc ccgacgacat
    13621 ccgccggctc tctcagaaag cccgccagtt gctggccagc cgctccgggc cgcgctctta
    13681 ccgcgatgcg gccgatgaca acatagcgct cgccggacaa ctatgggagg cgcgcaaccg
    13741 cttttgcgct tcgccgcctc ctgacgaggc gctctcgcgg gttctggcgc ttttggccca
    13801 ttgaaatcgg ccggcgccgg cggagcggcc cggccgccca gcgtcttcca ggcaaggcgg
    13861 gctcattcgc gaaggtctcg gcccgctttg ctgctacagt tgaattacgg cccccaggag
    13921 cacatcatgt cgaatgttgc ttacgtttca cgcatccata tcgagcgcag agccggccct
    13981 ttgcgaatcg cacatctgcc gggtgaacgt cagcccgttg tcttcagcgt gcatggcgcg
    14041 atcgcggagc actacaagat ggatccggcg gccttcgccg agccgcatgc ttccacgatc
    14101 gactacgtaa tcgccgcagc cgccggctga atgctgggaa ccttcggcgg ggcgctggag
    14161 gcgcgccgca tcgacgctgg caacgggcgt ctgacggccg aggtgaccgg agaagtggaa
    14221 accgacgagg gagttttagt catccggcgt attcatgtgg caatgaagct ggccgcccct
    14281 gtgcagtccc gggagacagt ggaacgcgtg cacggcactt acgcgatgcg ctgccctctt
    14341 tatcgcacgc tccataaggc catcgagctg acctcgtcgt atacattggt cgaggagcag
    14401 tgatgcgcgg cgcgcggtga gaccccggcc ttgtttcgag tagtgcggct ccctccgggc
    14461 ttgcgaggcc gttacagtcc taattgctta cacaacctct ccacgcgctt tttgacggcg
    14521 ggatccatca cgatcggctc cggccagggg cgtaggaagc cttcctcttt ccatttgcgc
    14581 gtggcgtcga tccccatttt ggagccgtaa ttgggaagcc ggctggagtg atcgagagaa
    14641 tcaacgggac ccaggacaaa ctgaatgtcg cgctccgggt cgatattgtt gagggccttc
    14701 cacgctactt cccgtacgtt ctgaacatcc acatcttcat ccacaataac aatgcatttt
    14761 gaaaacatgg cctgaccgag accccagatg gcggacatca ccttacgcgc atggccggga
    14821 tacgatttcc gaatgctgac cagcatcaag ttatgaaaca cgccctcggc cggcatatga
    14881 atgtcgcaaa cctcgggcaa ttgcaagcgc atcagcggga ggaagatgcg ctcaatcgcg
    14941 ttgcccatgt agaaatcttc catcggcggg ggaccgacga tagtggttgc gtagatcggc
    15001 ttcttgcggc gcgtcacgca agtcacatga aaaaccgggt aatcgtcttc gagggaataa
    15061 aagccggtgt ggtctccgaa cgggccttcc gtgcgcagct caccgagatt tacgtagcct
    15121 tcgaggacaa tttcggcgtt ggcgggcact tccagatcaa tggtctcgca gggcgtcatt
    15181 tccacaggct cggagcgtaa aaaaccggcg aagagcatct catcgaggtc gggaggcagc
    15241 ggcagcacgg ccgaaaacat cgtcgccggg tcggagccaa cggccgccgc caccggcatc
    15301 cgggcgcccg cctccttctg gatatttttg agcgaccggc ggtaatgctc cgcgccctgc
    15361 ttgtgcgttt gccagtgcat gcccgccgtg cggtcatcgt aaatctgcat ccggtacatg
    15421 ccgcaattcc gcttgccggt atcggggttg cgggagaaga ccagcggcat ggtgataaaa
    15481 cggccgccgt ccttgggcca gcattggagc acgggaaagt ccaacagcga aaatttctcg
    15541 cgttggatga cctccttcac cgggccgcca cgcacggttc gaggaaagaa ggatcccatt
    15601 tcggccagtt tcggcagcat tttgattttg ccgagaacgc cctgcggcgc tttcatcgca
    15661 agatactccg agatacgccc ggccacatcc tcgatggaat cgacttcgag cgcgagctcc
    15721 atgcgccgct ggctgcccag cagattgatg gcgacaggaa tgtcgtatcc cttcggccgc
    15781 tcaaacagaa gcgcgggacc gcctttcttc actgcccggt cggcgatctc ggtgatctcc
    15841 agaatcggat cgacctcctc gctgacacgc ttcagttcgc cctgtttgtg caatgcgcgg
    15901 agaaaatcgc ggaggtcttg gtaggccaaa gttattcctc gatctccagg tccttaaact
    15961 tgacggtcat gttgggggaa tgcatttgca ggccgatggc cccgcgccgc gaacggttat
    16021 tggcggtctc ggccactttt tcgccgttca gcattacctc gatccgtggg cccttgcatg
    16081 tgatcaaaaa ttcgttccat tgaccggatt tgattttgcc gtcccgagcg ggagcgagat
    16141 catatatgct tcccgtggga ttcttttcat gcggcacatc aagtatttga atctcgtacc
    16201 ccagatgtgc gggggttcgc gcgccgcggg cgtgggaggg atcgcggatc gccacaccgc
    16261 tgttgccgcc gggcgtgatc cagaacttca ggcgcagccg gaaatcggaa tactcccggc
    16321 gcgtcacaag ccaccccgtg cccgggtttg cagggtccga ggagcccaca atcattttct
    16381 cctcgaccct ccaaacggct ttgccaacct gctcccaccc gctgaggtct ttgccattga
    16441 agagcggctt ccaggcggcg gccgcgctgg cgctgaacgc gagcatcaag acgaacaaga
    16501 ggctcggcgg gcgcgaaaaa gccgctgtac gcacggtcat agactcgttt gtactatact
    16561 tttttactca tgtccaaaac cgcttttctc tttcccgggc agggatcaca gacgccgggg
    16621 atgggccgcg aactggccga acgctttccc agcgcgcgcg aagttttcga acgggccgac
    16681 gctgcattgg ggttcaagat ttcgcagttg tgcttcgagg gtcccgagga agcgctcagg
    16741 ctgactgcca acacccagcc ggcaattctc acctgctcga tcgccgcctg ggcggtcctg
    16801 cgcgagcatg gggtcgcagg ggactacgtt gccggccaca gcctcggcga gtattcagcc
    16861 ctcgtggccg ccggctcgct cgcgctggag gatgccgtga agctggtacg gcgcagaggc
    16921 caatacatgc aggaagcgac cccggaaggc gtcggcgcga tggcggcgct tttgggtttg
    16981 cctaatggcg ctctcgccgg catctgcgcg gaagccgcag gcggcgagat tgtgagcccg
    17041 gcaaatctta attcaccgga ccaggtcgtg attgcgggcc acaaaggagc cgtagaacgc
    17101 gccgtgctgc tcgcccacag agccggcgcc aagcgcgccg tcatgctgcc ggtgagcgcc
    17161 ccttttcact gccggttgat ggagccggcg caacagcgcc tggccgccga tctggcggct
    17221 atcccgttcc gggacctaca aatgccgctg gtgaataact gtgccgcgcg ggagattagc
    17281 accggcgatg aagcccgcca gggtttgatt gaccaggttc caaaccccgt gcgttgggag
    17341 gagaccattc gctatatggc cgcccggggc gtctcccggt tcattgaagt gggcccggga
    17401 cgcgtgcttt gcggattgct gcgcagcatc gaccgctcgc tgaaaggctt taatgtagaa
    17461 gacgtgaaag gtctggagaa gcttctggca gccgagtacg tatgaatact cctaaatacc
    17521 gcggccgcgg ccatattccg atctgatttg cgtccaactg ctttaccgtt atgcaaaatc
    17581 gagtagcgtt tatcaccgga gccagccagg ggatcggccg ggcctgcgcg ctcgcgctgg
    17641 cgcgccgggg agcgcgggtc atcgcggctg cccgccagca ggacaagctg gagcaagtgg
    17701 ttacggagat ctgcaacgcc ggaggccgtg cagcggcggt ccccctggac gtcacctctg
    17761 gggaatcggt cgcttccgct ttccggcaag cggaagcgct gttcggtaga attgaaattc
    17821 tggtcaacaa cgccggggtt acgcgcgatg gcctcgtgat gcgaatgaaa cgcgagaatt
    17881 gggacgcggt gttgaacacc aatttgaccg gctcctttct gtgtatccag caagtgctcc
    17941 ccgggatggt gcgggcgcgg tgggggcgca tcatcaacat cagctccgtg gtgggcgaaa
    18001 cagggaaccc gggccaggcc aactatgtcg cggccaaggc cggattgatc ggtctcacca
    18061 aggccgtcgc ccaggaggtt gcttcgcgcg ggattacggt caacgctgtc gcacccggct
    18121 tcatcgacac ggccatgacc gccgcgctcg agccggcggc tcgcgagcgc cttttgccgc
    18181 tgattcctct cggccgcatg ggtacggata tggatgttgc gcacgccgtc tgttttctcg
    18241 ccagcgacga ggccggatat ataacgggcc acgtgctcga cgtaaacggc ggattgcgga
    18301 tgtaaaggtc aacgcagggc cgccccggcg cgcctctgcg ggcagtcgtt tttatcatga
    18361 agcgctccgg atgcgccgat ttgcccctcc acggaggccg cgtgccggca tggctcgccg
    18421 ggcgcatgac gaaactcggc gcggccatcg ccgagagcgt gctctacgat tacggcccct
    18481 cagccttgct ctcccgcctc agcgacccgt tctggttcca ggctctgggg tgcgtgatgg
    18541 gcatggactg gcactcctcc ggcatcacta cttctgtgct cggcgcgctc aaacgcggcc
    18601 tgaatccacg ctcccgggag ctcggcatta cgatctgcgg aggccgtgga aggcattccc
    18661 gaaacacccc ggcggaattg cgggcagtag cggagcatac gggtctcgac ggagacgcgc
    18721 tggtgcgagc cagccggctc accgcccgga tcgacaacaa cgcaattgcg gacggcttcc
    18781 aaatctatct gcacgcattt gtagtgaccc gtacgggcga atgggccgtg gtgcaacaag
    18841 gcatgaatga acggtcgggg ctggcgcgcc gctatcactg gcattccacc ggagtccggg
    18901 atttcacaga ggagccgcac accgcgattg tcggcggcgc cattcagaac cgcatcatga
    18961 accttgtcga tcgggaggcg aaagcggcgc aaaacgcctt gctggcgatc gtccgcgaac
    19021 gccccgaagt cacgatcgca gaggcccgga agctgtgtgc gccgaagcat cacgatgtgc
    19081 gggcggaaga cgtcgatctg aaacggctgg gcgccgtcct ggcggccgcc tatgagcagg
    19141 agtttcggga tttcgcttcg tttctgctgt tggaaaacct cggcccgcgc acgctacagg
    19201 cgctggcgct gatcgcggaa gttgtgcacg gaacgccgag ccgtttcgag gaccccgccc
    19261 gatactcgtt cgccctggga ggcaaagacg gccatccatt tcctgtgccg cttggaacgt
    19321 acgatgagtc gatcgccgta ctgcgccgct cgctggacgc tgccaagctc ggccataccg
    19381 aaaagcttgc cggcttcgcc cggctcgacc ggttcgttcg catggttgaa gagaaccgct
    19441 ctccacaggc tcaatttgag gcgctgattg cccatgaaca ggcgatttct ccccggcttg
    19501 gcggtagaag cgtttcccat ggcccgaaaa tcaccgcccc caccgccgca ccgaaacaat
    19561 tgcggctgtt ctaaggcaat gcattcgagc caactccttg cactgcgctg ccagagcggg
    19621 gctgctgccg gcgcggcggc cggccacgcc gtgcgtcaga aatcgcggca gccgaggggc
    19681 attttccgta tccagacccg cgcgcgcagc tcaggcggga cgcaattgcc ggtccccatc
    19741 ggcgtatgaa aaagaagctt ctcactgtgc tggctttcac aggcggaatc ttcctggctc
    19801 tggaggccgg cgcgcggatc ttttccagcg acggccgcgc gggcattctt tcccagcacc
    19861 cggggcttgc gatgtacgcg ggcattgagc ggcccgaaga agtcttctcg ctgttgaatc
    19921 cgggctgcct cgagtggtcg ccttatcagc actgggtcgt gaggcccaat cttcgctcac
    19981 gcttttatcg caccaacgca ttgggcttgc gcggcctgga aacggccatg gaaaaaccgg
    20041 atgggcggtt tcgcatcgtc gttctcgggg gatcggcggc ctgggggctg ggatccacag
    20101 cggacgagcg gtccctgccc ggccgcctgg aggcgcacct gcgcgagcgg aatccgtcgc
    20161 gcgaactcga ggtgattaac gccggccagc ccggctacac ttccggtcaa gaactgattt
    20221 attttcatcg cctcatttcg cggatgaacc ccgatctggt cctcctgttc gacgggtaca
    20281 acgatatcga tgctgacctg cacaacagcc aacccggctg gcccgaaaac gcaaacatcc
    20341 tccgggcgcg ctacgatgca tccttcagca aaacccgact cagccaagag ttccttgacc
    20401 tgctgcgacg cagccgattt cttcacctgg cgagccaaaa attgggcgcc ttttccccgg
    20461 cgggggaagc ccccggagag accctctttg cgccggaggt cacggcgatg aactatgttc
    20521 ataacgtcaa ggctctggcg cggctcgctt cgcccgcccc tgtgtgggtg gcgctacagc
    20581 cggtgctcgc cacggtccgc aagccgctcg ccccggagga gcgtgaaatt ctcgcggcga
    20641 aagaaaaaat aatccctgat tacacggagc gtgtgaggcg gacgtaccaa ggcatagaag
    20701 aacaggtgcg ggcggcggcc atcccgttcc tcgacctgaa tgcggcgtta ggggagcagc
    20761 ccaggttgct gtttgcggat gaatgccact tcggcgacga agccgccgac gacctggccc
    20821 gccggatcgc cgccgcctgc gaacggctga tccagtagcg ccgcgcccgg cgaccggctg
    20881 cgggacgggc gaacgcggaa cgctgcgcag cgcggcggaa gcaaaaattt cgtgagaatc
    20941 ccctaacgaa ccgtggaatt gtggataatt gaccccggag gagggctttg tgaagcgcac
    21001 gtgctgctgt tcgcgacggc atgggccgcg cctgccagct cgggcgcaat caatacgctg
    21061 accccgcagg aatccgccca agactggaaa cttctttggg ataagaaaac actcgacggc
    21121 tggaagatca acaacggaaa gaacgtgctg gatgcgcatc attccgcgtt ccagagagga
    21181 gccatcggct tgcagcacac cggccagaaa attgagtttc gcaatataaa gatcaggccg
    21241 ctgtaagatc agggtccatt ggccggggac gcctgctgcg ggtgcggcaa gccgcactcc
    21301 cggcgcttgc ggccgcagcg gcggttggtc tatcctttga tccggccgcg aatggttgat
    21361 gcggcggcca acagcgggct aaatcaaatg atgcaggaac gcgtaaatcg acctggaggg
    21421 tgggtatgaa acgagcatac ttattgactg tggcggcagc ggcggcgttg gctgcggccg
    21481 ccctatttgc cgcgccggcg ggatccggcg ccgtgaacac attgactcca caagaagctt
    21541 ccagcggctg gaaacttctc tgggacggca aaaccctgaa tggctggaag cagtatggcg
    21601 gcgcccaatg gaaggtcgag aacggagcgc tgacgccttc cggccgttat ggatggctgg
    21661 ggaccgaagg cgactacgac aacttcatgc tcaaggtcga tttccgcacc tcggcttcga
    21721 acctgaacag cggaattttt ctgcggcggg gccgcgatga gaaggattct caccaaatcg
    21781 gctacgagct gcaaatccgc gatccgcagc ccaatgacaa gccctatgac gggaaaccgg
    21841 acaaccacaa cggctattac acgggcagct tctcgggcca tctcaagagc cacaacgagc
    21901 ccaaaatcgt gctcaacgag tggcacacct ttgaagtgac cgcgcagggc gaccatttcg
    21961 tggtgctcta tgacggcgcg aaagtactgg acgcccatca ttcggcgttt aaaaacggag
    22021 ccatcggact gcaatacacc ggccagaacg tcgagttccg caacatcaag atcaaacctc
    22081 tctaagccga agaggcccgg tggtccagcc ggcacggccc ggctggacca ttttccgcac
    22141 agtcagtttt cagcggccag aggtcgggcc tgtcttgttt ggccaaatgc catggcgggc
    22201 gccagcctgc aaagcggttc tctcccaggc ggcttgaaac gctaccagcg ctgcacagca
    22261 aacagctcgc ccacggccct gagccgcatc gcgtatgata gcgagagggt gcgggaatcg
    22321 cgtcctggcg caaccactta tggcggacca aacaagtcgc cctaccgctg ccgaccacgc
    22381 gctgattgac ttccatcgca tatccgtgat gcgtggtgag aagatcgtgc tcgacgatct
    22441 cacgttccgg gtggacatcg gcgaacatgt ggcgatccta ggtcccaacg gctgcggcaa
    22501 gagcactctc atcaagacca tcacgcggga atgctatccg ctccagcgcg agggatcccg
    22561 catgagtatt ttcggtaaag aaacttggaa cgtttttgac ctgcgttcac tgctggggat
    22621 tgtttcgaat gacctgatgt cgcaatgtac ccgcgacatt acaggccgcg atatcgtcct
    22681 ttcgggcttt ttcagcagca tcggggtctg gccccatcac caggtttccg ccgcgatgcg
    22741 cgaaaaggcg gacgaggttc ttgagattct cgaggccccg catcttgcgg gccgcccagt
    22801 caatgaaatg tccagcggcg aagcccggcg catcttgatc gcacgcgcgc tggtccacga
    22861 tccgcgagcg ctggtgctcg acgaaccgag taacagcctg gacgtttgcg cgcagcacga
    22921 gctgcggcag gtcatgcgga aactcgcgca atcgggcatc gccattctgc tcgttaccca
    22981 ccatcttgcc gacatcattc cggagatcga ccgcatcctt ttgatgcgcg aggggcgcat
    23041 cgttgccgac cgccccaagg accggatcct caaggcagag tgcctcagcg atttgttcgg
    23101 ggtccccgtc gaacttggcc gccgggatgg gtattaccac ctgtggtgag atcggctgcg
    23161 ccgccccctt gacggctgtt cggcaaggat agaccgcgtg ccccgcacct gacgtcgagg
    23221 ccaagtcggg tttgcgctgg cgcaatattg cccggccatt tctgccccag cgcgtttact
    23281 tacggctgat gatcacctgc tctggagacc tttcgagcga acgaatttcg ctgtgccgga
    23341 atccagccgc gcgaaacatc tcttcaaatt cggaaaaagt gtaagcgtct ccggtcggcg
    23401 ttgtggcgag cataatcagg ctgaacgacg ccggtatcgc aggcgaaacc cgatcttcgt
    23461 tgggtacgaa ttccagggta acggcaatgc cgccttcgtt cagcgaccgg tgcaccttgc
    23521 gcagcagatt ttcgatcgtg ggcgggtcaa agtggtgcag aaaattggtc agcagcgcta
    23581 cgtcgtagcc ggagccgaaa tcgacatcaa aagcgctgcc aggtaaagtc cggtagcgcg
    23641 aagctacgcc ggccctttct gcattttcct tcgcaacttc gagaacgctc ccccaatcca
    23701 cggctgcgac ttcggcgttc gggttccgcc gggcgatagt gatgccaaat attccgtggc
    23761 cggcggcgat gtccaggacc gtgcatttcc ggccggcatc ggccccgata agtccggcaa
    23821 tctcctcggc tgcgggcgcc atcagcggga ccatgctgcg ggcgaattcc acccataccg
    23881 cgttctcagg accgatggtt ccatcagggc tcatggctgt tcccccattg cgcacggcgg
    23941 ccgccagatc ctgaaaggcg ttgatcagat ccggagaatt aaggaacctg acagcgctcg
    24001 ccatgcacgc tggcgaattt cggtcgagga aaagggctga gtcttgcgtc aaactatacc
    24061 gcccgcgatc tttgctcagc aggccggcga tcaccaagta atcgcacaga atccgcacgc
    24121 cgcgctcgga ggcgcggcag cgctcggcta aggccttcgg cgtggccgct ccttcgccga
    24181 tgcccgtgaa catgtcaagc tcaatggccg ccctcaacgc cgccgtcttc tggtacgcat
    24241 tgaaggtctc aaaaatcagc gccggcgtcg gcgcggctgc tggggaacct tgcatcgctg
    24301 atattcctcc tgtgctattt tgcggcccta ggccgccgaa aagataagcc cttcgagtgg
    24361 ccagccgatc caccccgcgt aagcgccaaa cctacggccc gtttccggcc ggcaatttca
    24421 tccctctttt aaattctccc gcaaggaagc gccgagcgcc tcaatatcgg cgccttcagc
    24481 gcgcttttcg gcttggcacg gtgttcttat aaattttgcc ggccttcatt acgaatacga
    24541 cccggcgcac cgccgtgatg tcctcgagcg gattaccctc ggtggcgacc aggtcggcct
    24601 gcatgccggg tgcgagggag ccgatttggc cgccgagatt gagggactcc gctgccaggg
    24661 acgtggcgct gatcatggcg tccatcggct tctggccccc gtctttcacc cgataaataa
    24721 attcctcgta gttgcggcca tgcacgccgg ctacagcgtc cgttccgaat acgattttga
    24781 ctttctcggc cgccgccttg cggagcacct ctgccaccag cggaaggcct ttttgcatgt
    24841 aagcgaagcc ttcttcggtg taattgccga tacccagaaa cttcggctta ttatccaggt
    24901 aattgtgcag tacgaggaaa ttgggatcga agtaaacgcc gcgctccgcc atcaggcgaa
    24961 gcacgccttc atcgagaaac acgccatgct caatagaggt cgctccggcc agaatggaag
    25021 cgcgtgcccc ggccgacgcg tgcgcgtgaa ctacaatccg cttcccttgc gccttcgctt
    25081 cggcgcacgc ggcctggatc tgctcatcag tgagtgattg cgcacccccg tcacgaatgc
    25141 ttttggtcgc gaacattttg atcacatctg cgccttcggc cgaaactttc cgaacgaaag
    25201 cgcggatttg atccggacct cccgtggcct cgtcaatagc gcccagtgaa gtcagaatgc
    25261 ggggacccgg gtccgagcct tggttgatcc agtcgcgcag atcccggtcc atcggagacc
    25321 ccaggctttg tacggtggtg aagccggcga gcaaatcggc gtacgcgttg cctagggcgt
    25381 acaaaatatc ctgctgcgga gtgtcgcggt cgggctgggc caagcggcca tttttgtcga
    25441 agtgccaggt gagatgaacg tgggtgtcga tccatcccgg catcacagtg aggccgctca
    25501 gatcgtaggt ggcggcgccc ggaattcctt tcgagtctat cctgaggatc ttcgatcctt
    25561 gaatcacgat gctggcattt cgcaggactt tccctctgcc gtcgaaggcg gagccggctt
    25621 tgatcacgat cggcgcctcc tgtgcggcag cgctcgagag cgaaacgagg gccaaaatcc
    25681 agacgagatt gatcggtgat ttcacggcgc gactcctggg gggcgcgcaa gatggaaacc
    25741 ctttacggaa tgaggcattt tagcatgaag cccatgcggg gccgcccgcg cggtgaaacg
    25801 actggcaaca gaatggctcg cgcggcgtgt cttcgctggc agggcggcca gcattgcggg
    25861 ccggcaagcg tgtggctgcc ctgctgaaac agaagcacgc cggacgcgct caggcgccgg
    25921 ctggagattt tgccttcgaa gccgccgctt tgtagccggc ataggaccaa taaagagcgc
    25981 cgccggcaag cagcgcgatg ccgcccgccg cagcctggga acgtgaactg cctgtcagca
    26041 accaaatcga aaccgcgacc gcaagcgccg ggatcaccgc gcctcccggc agcgtcaccc
    26101 tccgctcagc cgccggcatc ttcctgcgca acaccggaac cgccaggcac gtggtgaggt
    26161 agtagagcag tcgcgcgata gcgctgagaa cggccagttg cgaaaaattg ctgaacagcg
    26221 ccatgcccca ggccgtaaga gcaaacaaaa tcatcgaaac atatggcgta tgaaagcggg
    26281 catgcacgcg cccgagcagt gcgggcaatt gcttgccctc ggccatcgcg tagagaatgc
    26341 gcgggcctac cagcagttgc gcgctgctgt tgcccgtggt tgacatgacc gctcccagcg
    26401 tgagcaaagc cgccccgagc gcgccaagga atcgctcgcc cgccgcggcc aggggcgccg
    26461 gagaagttcc gaggcccggg agcgtgccga gagcaacgat ctgtatcagc aagtaaatga
    26521 cgaccaccag ggcgatcgcc gacaccaaag cgatcggcag attgcgccgc gggttgatca
    26581 cttcctcgct gggcacggcc gcgtattcga agcccccgaa agcgaacatc agcaccaggg
    26641 ccgcgctgcg caaagaggtc aagtcaggca gctcgctgaa tgaataactt cgaggatcgg
    26701 caaaaaacag tccggcagcg ataaaaacaa gcaaaggcaa cagctttccg atcgtcagca
    26761 gattgatggt ccaggcgcca taacggactc cgagcacatt gaccagggcg agcgctccca
    26821 tcagcagcgt tatcgccgcg ctgcgccccg gacccgaggc tacggcgggc cagaagaacc
    26881 cgaggtaatc cgcgaaggca ttgcttaccg cggcggcccc cgtgaggcgg cccagcagaa
    26941 acatccagcc gacctcaaaa cctataaaac ctccgaacgc ttcgcgcgcg tacacataag
    27001 gacccccggc ggtctcgaag aggctgccgg cctcggcaaa acacagtaca attagaacag
    27061 ttgcaacgcc gcagataata tagcccagcg ggctggtccg gcccaaaaat ttcgccaccg
    27121 tagcaggcag tacaaagatg cctgccccaa tgacgccgtt cagcgcgagc gccgtcaagc
    27181 tgaacaggcc gacggcccgg actaactgtg gagaacagga ttgatccatg ccgcgctagc
    27241 cgctcaccgg gaacggccgc tctgctctat cgagaagcat aaagcaccag gatacgctac
    27301 gcgagcggct gcaagcgact tttgggtgga gtgaaggccc gcggcccccg cagaagaatc
    27361 cggaaaggac ccaacacgcg ccgttactcc gggtcgaacg cgcggtccat atgaatccaa
    27421 accacgcggg agaaacggac catttccggc acaaagggca aatacgccag aaaggcggcc
    27481 accagcaacc tttggagaga ccaattcgta agcatccaga acacgctcat cagcgccagc
    27541 acgggaggga tcgaaagaac gtagctcacg atcatggcgc cgacgaaata gccctcctcg
    27601 cgctcgtatt cgaggccgca gaccgggcaa cgctgatgca tggcgagcca gccctggagc
    27661 agagagccgc gaaaaatggg accgcggcgg caccgggggc aggaataaga aagcagggct
    27721 cccagggcgg aagtggcggc gctcaccttc tcaggataca accggaatag aagctgcgag
    27781 caacgctccg ctgccggcgg acgcgggaat gcggccgggc tttctgttcc gggggcgccc
    27841 gggcggcgcc accgtgtctg ccggcgcgag cagtcgcggc catattctgg ctgcatcccg
    27901 gctcgattcg caatagaata ggccattatg gaactcaccc gcagaaagtt ggctcacacg
    27961 gccgccttag gcgcgagcgc tgcgctgctc ggcggcaaga aggccaaggc gcaagcgcct
    28021 ggcgaaggcg catccatgat ggccgcgagc agcggcaaac ttgcccgggc ggacggcctg
    28081 actcgccaag tggcggagtt tatcgtgcga acaggttaca cagacctgcc tcaggaggtg
    28141 atcgacttgg gaaagaagtc gattctggac ggccttggcc tggcgctctc gggctcggtt
    28201 gccgaaaccg gcgggctgac gcgcgagtat ctgcaatcgc tggggctcgg ccgcagcaat
    28261 gcgactgtga tcgggtcgcc gataaaggct ccggtacggt tcgcggcttt cgcaaacggc
    28321 atcggcattc acgccgacga ttacgacgac acgcaactcg cggtggccaa agaccgtgtg
    28381 tatgggctgc tcacgcaccc tacagcgcct gcgctcccgc ccgcacttgc gtcggcggaa
    28441 gcagccggac tgccgggacg cgatttgatg ctggcctacc acgtcggcgt cgaagtggaa
    28501 tgcaaagttg ccgaagccat ttccccccgg cattatgaag acgggtttca ctccacgggc
    28561 acctgcggct gcatcggcgc tgcggcggcg atcgccaagc tgcgcgggtt caatgtcgag
    28621 cgcacgctgc gcacgctcgg gatcgccgcg agccaggctg ctggtttgcg cgagaatttc
    28681 ggcaccatga cgaagccgtt tcacgccggg cgcgccgccg aggcgggggt catcgccgcc
    28741 gacctggcgg cgctgggatg gacggccgcc gaccagatcc tcgaggcgcc ccgaggcttt
    28801 ttccacgccg ccggcggagg cttcgatccg aatgccattt ccggcaagct gggcaacccg
    28861 tggacgttcg tttcgcccgg cgtttccatc aagccccacc cgtcgggatc gctaacccat
    28921 cccggcatga cggaaatgct gtttctgatc caagagcaca ggatcacgcc gcagcaggtg
    28981 gagagggtgg atgtgggcac aaaccgcaac atgcccaatg ccctgatcca tcaccagccc
    29041 acgaacgcgc tccaggcaaa gttcagtatg gagttctgca tggctgcgct gctgctttac
    29101 ggcaaggccg ggctgagcga gttcaccgac gaggtcgtca accggccgga ggtgcaagcc
    29161 atgatccagc gcgtgcattt tggcgtgcat cccgaagccg agagggccgg ctacaacaag
    29221 atgaccacga ttatcgacat tcacctcaaa gacggccgca ccatttcggg ccgctccgat
    29281 ttcggcaagg gcagtccgga aaagccgatg agcttcgacg aagtcgccgc gaaatttctg
    29341 gattgcgcag cttttgcgaa ctggccggca gagaaagcca gggccgtggt cgaactggtg
    29401 cgaaagctcg aagacgtttc caacgtccgg gcgctcacgg ccctgctctc ggcctgagca
    29461 ggcaactcga gcttgaaatg tttataggcg caaagcgcct cggctctttg gaggattcaa
    29521 aactcgaacc gcatacttat ctgcgccagg cgcgcgttcg agcccacgac cacacccgtc
    29581 accaagccaa aattcggcag tccccgcttc aggttcggat tggcgaattc agggcgattg
    29641 ggcaggttgt aaaaatcccc gcgaacttga agtttgaagc gttctgtcaa tggccagttt
    29701 ttgtgcaccg acatgtcgat gccgaggaag cctgggccac agcagattgt acgaggcgaa
    29761 tttccaaaaa ctccgacgcc cggcgcctgg aactgcccga catcaaaata cggaattgcc
    29821 agtacgttgc tcctccagcc cgctggttgt aagggcggac caagcaagtt cggccgttgc
    29881 gaggctgaaa acgtatttga caaatttgtg ttctcgatcg ccccgtaggg tgcgccgtcg
    29941 cggatctcgc cgatcaggcc tattccccat cctccgagag caccgttcac aaaacggttc
    30001 ttaaggaggt acggtcgtcc agtgccccag ggaaggtcat agacgacgct tcccacgagc
    30061 cgccgaccaa tgtgattgcc agagagagat ttatcgagcc tgtgcaaggc gatgtgcgtg
    30121 taactgacgt tgccggccgc gccgccgatc tcggagttgg ccgagacgtc atcaatgaac
    30181 ttcgaccacg tgaaatttgc cagtaggttc aaaccggacg aataacgctt ttccaccttc
    30241 aggttcatcg cgtgatacgt cgaattaccc cacgccgggc taatctgcat tacgttatcg
    30301 aactgcggga aaggccgggc agtctggctt tgcacggcag ggcccttgcc cttgatcaag
    30361 gggatcatat tgatattcac gtctggcccg cccagccggt gcgccacatt ggccaggtat
    30421 gtgatctcag caacagaatt cgccgaaagt tgcttctgta tgccgaagtt ccattgctgg
    30481 gccatgggat caggctggtc ctgctggagg aaatcaggac tgaggatcac ggggctgccc
    30541 accgggacgg ctccgaaaga cgcgtcgtgg ggagcgggca tgatgctcgg catcccgttt
    30601 tgcaactgga atgccggggt aatattgccg tcaggcgagt tgaaactcgt tgagatcccg
    30661 aatcccgcct ttagcgcaaa agtcacacgg acctggtagg cgttgtagta gctgatacca
    30721 taaccgccgc gcagcacaaa tcccccgccc aagctgtaag caaacccaaa acgcggtccc
    30781 caatcgtgat aatcgaaatc atgggcatac ttgctcacgc cgtttacgcc agcgaacgtc
    30841 acgatgccag gcgtcccaga gacggggttg attgcagtca ggttaaaccc gctctggtag
    30901 ttgtggcgct cccatcgcgg ctgattcagt tcgtaacgca ggccaagatt taatgtcagc
    30961 ttgttggtga tcttccagtt gtcctgcaca aaaccggcgt aataatcact acggctcgca
    31021 agcgggacga ttgcctgcac ctcccctgta gccacattgc ccagcagcag ctctgctagc
    31081 cccgaaccag tggcgcgatt atcgaatacg aaatgacctc ccttgtaacc gttgaacagg
    31141 tccacattaa gcccgaaccg atattcgccg ccgagcttga taaggtgcct gcccctggat
    31201 atcgaataat gatccagaaa ttgccaagta tcgatgggcg tctgaatgcg ctcctgctgg
    31261 gtcgtgccga tagagcttag accattaata tcgaacgagc cgaaataatt cggatctact
    31321 cccctgatgc ccagcttccc gttttccccg ctattgactc cgaactccgt caacacaaac
    31381 ttccggcgcc caaaattgat gcgaacatcc tgaattgtag tgggcgaaaa gttgtggatc
    31441 cagtttccag tggtgttggt ctcctgattt tgctggctgt tcccgcgcgg atcagcaaat
    31501 gcgttcggga aaacggccgt agtggtcccg ggcgcacggt taaagataaa tcgaacgaag
    31561 actctatcag tcgcccgaaa gttgtgatct accttggccg taagccagtc ctgggtcgtt
    31621 gcgttgcttc ctccgttgat aaagttgttt ttgggcgcct gagtgctggg gctggggacg
    31681 ttcggcggcg gataaagttg tgcgattttc cgcccgacgg gatcaatccg ggctggtgga
    31741 attatgttgt tcaaaaaggg ctgtcccgtg gttggatctc tcaggaccag atccctacgg
    31801 ttggaaaaat cgcccgtgac ttccggcggg tttggcacca cgtcattgta cgtcacgcca
    31861 tcgcgtcttc gggcgccttc atagttcgcg aaaaagaacg tgcggtcttt ctttatcggc
    31921 ccccctaacg atgccccgaa tatgttgtaa acgaggggcg gcttctgttt cgcaaaaaaa
    31981 gtcctagctg cgagcgactg attgcggaag aattcatacg cggcgccgtg gtactggttc
    32041 gttccggagc gcgtggtgat cacaatgtag ccgccgccgg tcctgccata ctcggcggaa
    32101 taattgttct cttctgcctt gaactcctgt acagactcgg cgggcgggtt cagcgtggtt
    32161 tgctgcacgc tcatagtgac gttctgccct gaggtgccgt ccaacgccca catctggtta
    32221 actgaacgcc cgccggacat tgagaagacc ggttgtgagg cgacagtctg cgtttgcaaa
    32281 aagacgacac ttcccatcag gcgaaccaag cccgcgctgc ggcggctctc caacggcata
    32341 ttggaaaccg tttgccgctc gaccaactgc cctacagagg cagtctccgt ctcaagcaga
    32401 ggggcagagg acgtcacctc gacagactcc gtgacgccgc ctacttgcag ctctatatcg
    32461 aggcgacgga agaatcccgt atccagcgta acgcctcgct gaaaatagcg cttgaaaccg
    32521 gttgcctcgc agccgagttc atattcgcct gccgcgagca cggggaagtt gtagattccg
    32581 gcggcgttgg tttctgttgc tagctcaaca ccggtgttca tgttacggac tgtcactctc
    32641 gccccggcaa ttactgcgtg agaactgtcg ctcacctcac cggtcaagcc agtctgaagc
    32701 tggccccagc agatcacgat ggtcgcgctg acaagcgccg gcggaaacag tagtcggagt
    32761 acgctaagca gtttttgcat acggctccct tcttgttcaa gacctaaagt cgttcgctcg
    32821 ggtcatgatg aacgaggatg ccgaatgaaa cttattttcc ctgccggaac cgccacctac
    32881 aaatccgacc tagcggtcag gcgttaagct ggtataagca gaattgaaag attccccccg
    32941 ccccccgcta cgggataacg gttccaaaaa acaggactcc gattggaact gcacactcac
    33001 ttccccgggc ctcaactaag accatgcagg gaagtgtact cctgccgttg cagggaatca
    33061 agaagattat ggctgctcga aatggccggg agcagtcggg ccgaatgcgg cttcacgcag
    33121 cacgcacgtc catggcaaca aatgctgaga tcgccgtgga agcctccctc ctagggcgcg
    33181 ctcccgctcg cggggggccg gatgcaagct caggcaagaa gaggcgtgcc tttttcttcg
    33241 cctcagtcca ccgagaaatc tgctgtcgct acggttcgct cccaggccat tttcaggacg
    33301 ccgcgtttgg gtcccgcggt ctcgagcgaa attgtaaact gctctatggg cgatccggac
    33361 gtggtaacct tcatcggcgt ccgccccagg tctttggatt gatcgtagtt gaacgcgccc
    33421 cactgtttcg ccactttgtt caccaccagc gtccaggcat tcgcctgcgg aatcacgaac
    33481 agggcgtagc tgcccttcgg cactctcagg ctcccgatcg tcagatcggc gtcagtggtc
    33541 aacactgtgg cctcgtcggc tcctgctcgc catacctcgc ctaagggaac cagcccgccg
    33601 aagatcttac ggcctttcat atacggccgc ccatattcga tagtgatttt cttgccgtcg
    33661 atcgttactg aagtctgctc gtgcgggctg acgcgctgct gcgccattgc gggaaggcca
    33721 agcaatgcgg ccgccgccag aaccggaagc atgtgaagtc tggccatatc aatctcctcc
    33781 tgcgtatctt tccgctcatc ccattctagc gggttacgtt ctctgccaac gcgaacgaaa
    33841 tcagcgcatg gccggcggcg acggcaacat gttgtttccc gtcgctcaga taggtgatcg
    33901 gattggcgaa aatcgctccg cccgccggaa accgccagag cggcttcccg gtcgccgcat
    33961 cgagggcgaa aaggtagcct tcgttggcga cgccgaaaac cagtcccccg gcagtggaaa
    34021 gcacgccgcc ccacggggga gactgcaagt ggaattccca ccgctttcgc ccggtttcca
    34081 cttccaaagc ccgaatgtag cccgtcggct ccactcccgg cagcccgcga aagctgccgc
    34141 cgacatacaa cgctcccggc tggtaaatag cctcgcctcg aaaaaaaatc gtggattctt
    34201 cgcgcactgc cacgtaaaac aggctggtct gcgggctgta ggacgggctg aaccagttcg
    34261 taccgccatg aaggctcggg tacacgccgc tgccttccgg cgaaggctcc gtgttgggca
    34321 atgcaatggg gcggccgcga acatccagac ccttggccca ggtttgcttc gcatattccc
    34381 ggcccagcag aaactcgcca gtttggcggt caaggacgta atagaaagag ttgcgattgc
    34441 cggtcacaac cagcttgcgg ggccgccctt gaaaagcccg gtccacgagg accggtattt
    34501 gattggcgtc ccagtcgtgc gtatcgtgcg gcgtaaactg aaaatgccat tgcagccggc
    34561 cggtatcggc gtccagcgcg atgagactgc acgaatagag attgtcgcca gccctggatg
    34621 ttccgttgta gttaggaccg gggttgccca cgccccaata gatcaagttc aggtccgggt
    34681 cgtaggagcc ggtgagccat gtggtggctg accccgtttt ccagctttca ccggcccagg
    34741 tctcgttgcc cggctcaccg ggcgcgggta ctgtccagaa acgccagact ctcttgcccg
    34801 tcttggcgtc ataggcgtcc aggaaaccgc ggacgccaaa ttcgcctccg gccacgccaa
    34861 cgataatttt gtcctttacc gcgagcgggg ccgcggtcat cgtgtagccg ctcttgtagt
    34921 cggccgcggt cacgtcccag cgcacacggc cggtcttggc gtccagcgcg accaagtggt
    34981 cgtcgagcgt agagacaaag acggtttccc ctaagcccgc caagccgcgg tttacctggc
    35041 cgcagcagat gtgaatgtcg tcggggatcg cccgcgcata gcgccaaatg ggccggccag
    35101 tgcgagcatc gagagcggcg acgttgctcg gcggctcggt aatgaaaacc aacccgtcgg
    35161 cggccagcgg cgttgtttcg aatttgttgg gatcgttcac ctgatacatc caaaccggcc
    35221 gcagtccgct cacgttggcc ggagtgatct gggccagcgc cgagtagcga tggctgctgt
    35281 agtcgccgga atatgtgagc cagttggcgg ggtccgaaac cgcgcgcaag atgcgttcat
    35341 agctaaattg cgccgggatc gggagccgca gcgcggccag aagccagacc agcctcaatg
    35401 cggacgcccc gcggctcatc ggccgccccg caaggagact agataggcaa tcaggtcatc
    35461 catctcagcg ggcgtaaaga cgccccgata gctcggcata ggagacttgc cccaatcctt
    35521 atgcagttcg gccagctcgc ttttccaaaa ggagtagatg tggtccgaga agtcgcggat
    35581 ctgaatggaa aacggatctt cattcaggcg gatgcccgta atgcgctgtc cgtctttggt
    35641 cacgacgcgc acttgcagga aattatcggg cagtagagtg acgaagcggt agaggaagaa
    35701 attttccggg atggcccgct ctggattcgt tatggccgtc cgcaaatagc cgggactgcg
    35761 ccgctctccg atgtcggtga gctccgggcc aaggcggccg ccttcgccag caatcatgtg
    35821 gcactgcccg catttgccct tgccgcggta gagctcggcg ccgcgaaccg ggtcgccgtg
    35881 caccggcggg gaagagcgtt ggagcgtccg gacgtaggcg atcacctgcc agatatcacg
    35941 atcgagcaag tgccgcgtgt tgggcatttc cgttcccggt atccccctac ggatgacgct
    36001 gaaaaagatc cggtcatcgc tggcccgcgg caaatgagga cgggtcagat cggatccctt
    36061 gcctccatcg ccattcggcc cgtggcacgg agcgcaattg ctcaaaaaca ggcgcttgcc
    36121 ttcttccaag tcttccgccg tggggaaagg cgccggaggc ggtggcggag ggggctgcaa
    36181 ggcggacggc ggcaggggcg aggacactac ctgggcccag cccagcagat gtccccaaca
    36241 gattgccagg gtaaggaaac taatccaacg caaatgaaaa tatatcactt cctgaagccc
    36301 gtgacccggc gcccgatgag cagaacggcc cgatagctcg tctctaagac agccctctcg
    36361 catttcagcc ggccggtagc tggcggcgca cgttgagaat ttgcactctg ataatgctca
    36421 tggcgggcgc atccggtata tttcggatga tcggtggccg gcagccgccc tcttctccgg
    36481 ctcagtggcg gggatggatt tgcgagaatt tgaggccaaa ctcgaacgca tccggcgatg
    36541 gctcgccgca gagcggtacg acgcggtaga gatcacgcgc tattcctggc tggcctggct
    36601 gctcgaaggg gctgaagccc gcgtcctcct gtcttcggaa cgcggcaatt gctcggtgat
    36661 cgtaacggca gaaaagattt ttatggttgc caacaacatc gaagctccgc gtttaaaaga
    36721 ggaagaaatc ggcgagttgc ctctggaatg gagaatctac aactggtggg agcggccgcc
    36781 cagcgtgctg ccccaaaacg ccagagtcgc ttcggagatg cccgccagcc ttcgtttttc
    36841 cctgctcgag cccgaggtgg cacgtgcccg gaagctgggt cgggaggcgg ccgaagggct
    36901 cgaaacggct gctcgaagcc tctgccccgg cctttctgag cgtgagatcg cggggcgcat
    36961 cgccgcgcaa gctatgggca gaggtcttac tccggccggc ttgttcgtgg ctgccgatga
    37021 acgcggcaag caattccgcc atcccatccc taccgccaag atcgcatatc gttatgcgat
    37081 cttgagcctg gtcgcccgcc gccacgggct gcacgcgagc gtcacacgaa cggtttcctt
    37141 cggtccggct tgcggggaga tgctgcgtcg gcaccgcgcg gcgagccggg tgcatgcggc
    37201 gatgctcgtc gcgtccctgc cgggcacgcc gctgggcgaa atcttcgccg aggcgcagag
    37261 agtctatcga gattccggtt tcgcggagga gtggaccttc catcatcaag gcggcccggc
    37321 aggttacgaa tcccgcgagg ctcgcgctgc gcctggctcg gccgagcggc tgcaagaaaa
    37381 tcaaatggtt gcgtggaatc ccacgattcg cggcgcgaaa tcggaggaaa ccgcgctggc
    37441 ggccggccgg cgggtcgagt tgttgacccg taccggctgc tggcccgaac tcgagggaga
    37501 catgcctctg gccgatgtgc tcatgataga aagctgacat cgattttcca aacggcgtcc
    37561 ccgcctgtta tcctgcaacc cagagcgagt tggcaaatga cgcctcaaaa attgcaagaa
    37621 gccgctccgc ccgttatcaa gccctggtac accggcctga ccggctatca ctggtgggtg
    37681 ctgagcgtcg catccctggg ttggcttttc gacacgatgg atcagcgcat cttcgtgctc
    37741 tcgcgcgggc cggctctgat gtctctactc cctgctggaa cgagtccggc cgacatcacc
    37801 tggtatagcg gtctggccac ctcaattttc atggcgggct gggcgatcgg cggtttctat
    37861 ttggggatta ttggagaccg ctggggccgc gcgcgtacca tgatgctcac gattctcatg
    37921 tattccctat tcaccggcct ctccgcgctg tcgcgcaatt ggtgggattt tatcgcctat
    37981 cgttttctta ccggcctcgg cgtcggtgga gaattcgcag ccggggtttc gctggtggcc
    38041 gaagtcatgc cggaccacgc gcgcgcgcac gccctgggca tcttgcaggc gctatcgacc
    38101 gtgggaaata tcaccggttc cgccatcagc ctggtggtcc tccctctggg gtggcgctgg
    38161 atgtttttag tcggcatcgt tccggctctg ctcgtggttt tcgtattccg caccatgaaa
    38221 gagcccgagg cctggcaaaa agcccgggct tcggcgcagg ttggggctca aaacagaatg
    38281 gggtctgccg gcgatttgtt ccgggaccgc cgctggcgca gaaacacctt tatcggattg
    38341 agtctggcga tttcgggcgt ggttgggttg tggggcattg gtttctggac gccggaattg
    38401 atacgctcgg cgctggccag cgcgcccgcc gcatcgcgaa actggtatgc cagcatggga
    38461 acgctgctgc aagacgtcgg cgcgtttttt ggaatttatg ggttcacgat cattaccgct
    38521 cgcgtgggcc gccgcaaggc atttgtggcc gcattcttga ttggattggc ggcgaccgtg
    38581 atgacgttcg gctggctgcg gcggccgcag gacgttttct ggatgatccc ggtgctcggc
    38641 ttctgcaacc tttccgtatt cggcggatac tccatttatt tccccgagct ctatccgaca
    38701 aggctgcgca gcacgggcgt gggtttttgc tacaacgtgg gccgcatcgt cgccgcgctg
    38761 ggacccttca cgctgggcgg cctcacggtt gtttttgcga aagccggatt tgccagccct
    38821 ttccgggctg cggccatctt gctggcatcg atttacctgc tgggcgtttt cactatgcga
    38881 tacgccccgg aaaccatggg caagccgctc cctgaagaat aaggcgatct gtgaaaaatc
    38941 catgacggaa gcgaggccgc gaaaagcggc cgcagcgcgc ccggatccca acgttccggg
    39001 tctatgataa tggttggcgt ccggagtagg agccagcgcc gctaacttgg catgaagtat
    39061 ttcatttaca aaagccttgc cgagatcgaa gctgatgcgg agcgcatcgg ggcgaagcac
    39121 gtggcgttcg agcataaccc ggccaaggta caggccgtct tgagccagcc ggcggcagtg
    39181 ggaggcttcc gcgtaggcaa ttcgatcgcg attcatccca tggagggctg cgacggggac
    39241 ttggacggaa gccccggcga actgacctgg cgccgctacg aacggtttgg acgcagcggg
    39301 gccaagctgc tatggttcga ggccaccgcg gtccgcgaag acggccgcgc caacactcgt
    39361 cagttgtgga tccacgaagg cactgtcgac aagctgcgaa accttctcgg ccacacccgg
    39421 gccgtgcaca aagagctcta tggcacggcc gatgacctgc ttgaggtgct gcaattgacg
    39481 cactccggcc gccacagcgt cggccgaaag atgatcgcgg ttcacaatcc atgtgtggac
    39541 gccaagacgg gcgtgccggc cagctatcca gtggtcccgg atgaggaatt ggagcggctc
    39601 gaagacgact acgtcgctgc ggcgcggctg gcctggcaag ccggttttcg cgcggtagac
    39661 atcaaagcga cacatgggta tctggtggcc gagctgcttg gagcgaagcg ccggccaggg
    39721 cgatacggag gccctctgga aaaccgcctg cggctgctgc gcaacgtgat cggcaaaatt
    39781 cgctccgagt tggggaatca gctcctcgtt tgcgtgcggc tgggctgctt tgacggcgtt
    39841 cccttctacg ccgacgccgc aaccaataag ggcgtgcctt gccgctacga ggtcccctat
    39901 ccgcatggct tcggcgtcga tccggccgac ccgctgcgcg aggatttgac cgaggtgaag
    39961 caggccgtgc ggtgtttaga gtcctggggc gtggcgctgc tgaacatctc ctgcggcagc
    40021 ccctacttcg acccccacgt gttgcgcccc tgtgagaagc cggatgacgg aaactacgac
    40081 gctcccgagc atccgctgct cggcgtggac cggcatttcc gcatagcagc cgagctgcaa
    40141 caaaccttcc cccggctgcc gatggtgggc agcggctata gctggcttca gaagtacgca
    40201 atcaatgcgg gggcgaagaa catttccgaa ggaaggattc gattcttcgg gtcgggccgg
    40261 ggggcgctgg cgcatcctga tttcgcccgg gaagcgctgg agcgaggcga gctggacgag
    40321 cgcaaggttt gcaaaacctt gacgttctgc acgtttctca tgcgccagaa gaaccacccg
    40381 atgggacaat ttccttgcgg ctgcccgccg ttcgataagc aaggctatgg gagaatcatg
    40441 aaacttgcgg tggaagccaa gcggtcgcag cgcgcgccaa acagcctgcc ggcgggcggg
    40501 cccgaaaccg cgagaggcgg gaatgaccct gttgcaaatc gagacaagac acgttgagcc
    40561 ggacatcaca atcttggagt tcaccgggaa catcactgcc gcggagtcgc ggcgcgtgga
    40621 agttctggtt gaagacctgc tcaagcagaa ttacaggaag ctggtgttcg atctgaccga
    40681 agtgaactac atcgacagct ccgggatggg gagcattgcc cactgttttg cccgcgccat
    40741 ccgcaatgac ggagggcttc gcgtcgccgg cgtgaacagc cgcgtgaaac acctttttca
    40801 tctgacgcgg ctcgacgcgg ttttcccttt ttacgcgacc tccctggctg cctgcgccgg
    40861 ttttgaaccc gcggccaagc ctggcgagca gttgctatgg tgaagcagtc ggcgtcgtta
    40921 actggtgcgt gccgccgatt tgctgtatgc tacggccatg ctgcaaattg acaccaggga
    40981 gcttggatcg ggcatcacgg tcgtgacgct cgcgggcaag atcacgctgg gactcgagag
    41041 ccagcagatc gaatcggtgg tgaaggactt gctcaaacaa aaccggcgaa aattgatttt
    41101 tgatctttcg ggcattacct acatcgacag cacgggccta ggcatcctca cgttttgctc
    41161 ttcccgcgcc acagaagcgg gcggcaaatt gtgtatagcc ggagccgcca gcctgcccgc
    41221 cagactgttc cacatcacgc ggctcgatac gatcattgcg ctctatccca gcgtggaagc
    41281 cgcgcgcgaa tccctcgccg ccggcacgtc cggatagacg ccgccggcca ttttctaagc
    41341 gacggtctgc cagccaaacg cagtaccgct tccgggccgg agagaaccga ggcgggccaa
    41401 gaacgccgtc gaataaagca tccccaggat cgagagaggt acgaggacgc gcagcatcgg
    41461 caacgggctg gcccacaaaa cataccctac cggcatcagg agcacaatag ccagggcccg
    41521 cggccagcct tccgctgttc tcgcgtaaac aatcaggcag gcgggcagca ggagcacagc
    41581 gtcggcaacg gatccgtgaa cgccgatcag caagcctccg gccagcgcgg cggccaatcc
    41641 catttcgaaa tccgcgtttc gcacgatgat ccacacggca atgacggccg tgagccccac
    41701 aaggccctcc caccaggcag agtgcggcca gccgaaaaac aggccgcgca gattgggcat
    41761 ctgctccacc acatcgacgg ttgtcgtcag cttcagaatc tcccagtagc gggccggcca
    41821 gcccagaccc gccgtagcaa aggacagcgt cacgaagaac agcccgccgg ccgccagacc
    41881 gaacgcgaag cgcacaagcc gctgccccac aatcagcaaa gccacaagca gaaagagatg
    41941 aaattttgct gcacaaaaag aaaaaatcag gccagcccag aaatgcctgc cctcccgctc
    42001 gagacgcagt gacagtgcga tccagagcaa aagcaacggc aggtcctgcg cgttcatgaa
    42061 acaagcgaag agcggaagag aaagacaact gaacaatacg gtaatgggaa atggagtagg
    42121 ccgccaaaga gccagaaagc cggcaaaccc cgccagcaac agcaattgcc acaggagata
    42181 agcagtgtga taaggcatcc ggccaagcgg ccgaattaaa agagcgaggt agggcgggcg
    42241 gcaataaagg tagttttcac ccgtagatcc tgctgagtcc ccctgaacct ggcgcacccg
    42301 cgccggattg tacaggtccg gcgtcccgct tagcttggcg ccggcgtaaa acatcacaaa
    42361 atcgttcacc ccggccagga tgcggtgccg gggagcccac gccatcggcg ccataagcac
    42421 gatgccgcat aggcagccaa gaacgatagc gggatgaaat ctcatcctgt caatcagcgg
    42481 gacgcggcct ttcggcggtc ctgcctacat gccagccagc agcccatcgt tttaacagtg
    42541 gaaccgagtt caaacgccgg cagcgagcgg gtctgcagcg gccaggccgc gccgcttccc
    42601 agtctccagc ggcagcgggc acaattccat agggctctta acggtaatgc gtcagaaaaa
    42661 ggctctatct gccgactaga tatgttttgt atccagtcag gcaaagcacg cccgggatgc
    42721 aatggctatt gggaacgccg gcagcccagc ggcgagtctt tcgaaagagg taaggcggag
    42781 gagttccgct tttaattctt ggcgtagtag ccctggcgag cctgcacctt gaagtcccgg
    42841 ttgcggggga tgatcttgat tcgccggaat ccggggccgc tgcggggcgg agtgtatccg
    42901 atggaatact ggctgcggag ctcctgctgg atttcgtcgt aaatcatttt gaggttcttc
    42961 ttcttgctca cttcgaaaac tcttccgccg gtttcctcgc tgagcttttt gagcgccgac
    43021 ccgtttccaa aaccgccaat catgccgtag ccgccggccc ggtagtagaa tccgcgatcc
    43081 aggtactcga tgctgtagat gatggcatcc gcccgttgcg cggcggcaat ggcttgcttt
    43141 agatccatct ggctgccctg gtcgtttcca tcggtgatca ataccagcgt cttccggcca
    43201 acctggtttt tcagcatctc gtcggccgcc agaaacaccg cgtcgtaaag caaagtccct
    43261 ttcggtttcg ccgaggtcgg cattgggccg cctcccaatc cgccgctgcc cccgttgacg
    43321 tgcagcttgt cgagccccgc ccgcagaagg cgcaccgaat ccgtgtagtc ttgcagcaga
    43381 tccacattca catcgaagct gataagaaac gcctgatcct ttttcatccg cagcatctga
    43441 ttcaggaact gcgaggacgc ctcccgctcc aggtcaatca agttcccttg actcacgctg
    43501 gtatccacga gcaagcccat cgtaagcggg agatcagcct gccgcgaaaa gtacttgatg
    43561 gtttgcggct gcccttcttc ctcgagggtg aagtcgtttt tgtccaggtt ggtaatcaag
    43621 cggccttgct tatctgttac cgtgaaaaag atgttcacga catccacgtc tacggagatg
    43681 tcggggaggt cttcggattt ggagggttgt tgtgctgcgg atcgggcagg gaaccacagg
    43741 agcgcggcca gcaccaccgt ttccagtgtc aaaatagaaa gggcttgctt catcggctgt
    43801 cccgttcgtc ggaaatgcgg tttacgctca gttcgattgt agcagtttta gggtttgtct
    43861 ctctgggcgg cctggcggcc tcgcaagatg cccgctctgg agagaatgct ccgccggtca
    43921 cgattcccgt gaccgtggag gactcgaaag gagacctaat ctccggcctc gaagctgggg
    43981 attttcgcct gtttgagaat ggagtggagc agaaggtaac gggcttttcg gctgatgctc
    44041 agccgctttc tatcgccgtg gtgatcgacg cggcgctcag cggccctacg tccgagaggt
    44101 tgcgggccac tttcccgagc ttgctagccg cgctcagcga gtctgatgaa gccggcgtgt
    44161 tttcttttga tgttgacttt cgcacagtcg ccgagtttac ctcggataag gacaagatcg
    44221 agaaggcgct caaacaaatt cctctgggag cagattacgg tatagccggc gagccgctgg
    44281 gtgcgccggc cccggttatc aacgggcagc agatcggcgg ggcgcccagc cagtacccgg
    44341 gcgcggggca gcgccctcga aaagacatcg atagcgcttt gatttcggcg ctcgatgctc
    44401 taacgcaaag gcccgcagcc cggcgccgga ttgttctgct tgtgacggac ggggtgaatt
    44461 ctccccacaa tccgatttcc tcaagcaagg tgctggaaac ccttcggaag acggacgtca
    44521 cggtttacgc catcggcctt gacagcgcca agctgccccg cggctcgggc caactggccc
    44581 ggttctgcga agccaccggc ggagaactct tcaaggccgt gaagaaaacg tcgatcgaga
    44641 acctctatgc ccggatcact gagcaggccc gctatgcgta tttgctttct tatatgcctg
    44701 ccggcgcagc cgccggattc cgctccattc aggtttatgt cagcaaaccg cacgcaagaa
    44761 ttattgccag agagggctat ttagccggcc ggaggccgtc acagccgtga aaagagctgt
    44821 cgtccatctc agggctgtcg atccggtgct ccgcacaatc atcgacaagg tgggcccctg
    44881 ccgcattcag taccgcgagc cggagtttga gacgctggcc cgggctgttg tttaccagca
    44941 attgcacggc gcggcggctg cggccatctt tggccgggtt ctcgcaatgg cagggaaccg
    45001 cgatgggcgc cttacggcgc ggtcccttct ccggatcccg aaaaaagatt tgcgcgctgc
    45061 ggggctctcg gcgccgaaga tcacctacct tcgcgatctg gccgagaaaa cggctgctaa
    45121 gattgtccga tttgaccgct tcccaaatat ggaggatgca gagataatcg cgtctctgac
    45181 acaggtgaaa ggcatcggcg tttggaccgc tcatatgttc cttctgtttg cgctccgccg
    45241 ccccgacgtg ctgccggtag gtgattacgg agtcagaacc gctgtacggg atgcgtacaa
    45301 gctgaaggat ctacccacgc cggccgaatt ggaggcaatc gcacgcccct ggcgccctta
    45361 ttgcagcttc gcaagctggt atctgtggcg cagccgggaa ttgtagcttc tccagcccat
    45421 gcgggcactg gcgcgccgga tgcaaagcgc ccgttacggc aatccctgcg ggccacgatc
    45481 ggcgatcgct cttcagaggc tggtctcaaa ggctggcatt tccatcgaat agagaaggca
    45541 tccaggcgcg ccggtccgtc agagcgcggg ccagagcggc gtctttagcc aagtagaaga
    45601 acgtccacgt cgaaagcttc cggcgatggg gggcggcggc atccataaac tccccgcgct
    45661 cgagccaccg gccgacgctt tcaagcatgt ggatgccttc cgcccggcac tttcgcaccg
    45721 cccacgctag aaggggctcg agcagcgcag tagatccgtc aagggacaca aagtccacca
    45781 gtctcacccg cttcaggccg atctccggat tatcctggcg gtcgaaaatc gcgtacgccg
    45841 ctattttcgg accatgcatc gcggcagcca cccacagccg ctgatcgaga accgcgtgca
    45901 tgaaatgcca ttccagcacc tcttgcgtgc gcacggctaa aagcacccgc ggatggttcg
    45961 cccggaattc catccagaac ttctcgaacc gctgatcaaa acctgggaac gcccgtatct
    46021 ctgtatcgcc ctcttgcaac gggctctgcg tgacccaatc tttgagaaaa gatgcggcgg
    46081 aagcgggata gctcagcaac ctagtcatcc gatatttcct tttacaaagc acgctctcta
    46141 taaagcccct ctggttggta atccaaaacg cggattgatc ccacaatccg gagggaacct
    46201 tcgggcacgc gaagacttca aatgccgcag cggccggcag cgtcattgta ttgttgagat
    46261 ataaatcgat gcccggctga ttgatcaccc ggtctaacag caaaagcgac gcgcttcgat
    46321 aggcgggatc agcaacctgc gagcggcccg aagccgccag aatcctgcgg ccctcaaatt
    46381 catatagcag aggaatattg ccgacggatc cgacgatccg gccgttgtct tcgatgaccc
    46441 aaccgatttt ccaatgcggc agataccggc aagccggatt gccggcccaa aggtgcgccc
    46501 actgcctgta ggttttggcc tcgaggccgt atcgggactc gagcgccgca atttgggcgt
    46561 aatcgtcgaa agtggcttcg cgaagcctgg gccccgatcc ttgtccggcg cgggcgtcgg
    46621 ccggcagagt gctctttgga attttgtgca cctgccctcc ttaggccgct ttgaccaacc
    46681 cctgttgagc gcgaggcgcg gcttcgaacg ctatgggcaa gcgttccagc cctcggaaaa
    46741 ccatgttgtg gcgccaggcc agaggaacgg gatccagccg cagattgcga agacgccgga
    46801 ataccgttga aaacgcgatc tggccctcga ctcgcgccag cggcgccccg aagcagtagt
    46861 ggccggccca gccgaaagcg aggtgccggt tatctgtacg agtgatgttc aaacggtcgg
    46921 gatcaggaaa ccgttccggg tcgtgattgg ccgccccgat caccgcgatg accgcatccc
    46981 gcttccgaat cattttgccg ccgagctcgg cgtcggctgg agcaagccga gcggtgtgct
    47041 ggatggggct ttcgtaacgc aacaattcct cgacggccgc gggcagcagc gccggatccg
    47101 cctggagccg cgccagttcc ttgggatttt gcagcagcgc gagcaatccg ttgccgatca
    47161 ggttcgtcgt agtctcctgc ccgccggtca tcgtaatgat gcaattggcg atgatctcct
    47221 cttcgctgag ccggtcgctt tcgatttccg ccgacatgag cgcgtgcacc acgccttccc
    47281 ggggatgtcg gacttgttcg cgcattctct cccggaagta ggcggtcatt ttctccacgg
    47341 tggccagcat cagagccgcc cggcccgggt tgtgttgaaa attcccgagc atttcggaaa
    47401 aatccttcga ccacgtcttt agctgatcgc gatcgcttac aggaacgccc atcagctcgg
    47461 cagtcaccgt tgcgggcagc agatcggcga agtcggcgat cacatccatg cgcccttttt
    47521 cgatcactct gtctatcagt tcgtccgtga tttcctgaat gtgaaggcgt atcgcttcgg
    47581 ctcgggcggg agtaaacgca gcagcggcca gagctcgtaa tcgcgtgtga ttcggcgggt
    47641 ccatgaagat catttgccgc acaagcacct ctgcgaccgg attcagctcg gcaagtccga
    47701 gactggtgag ctgcgccggc gtgggcatgc gctcagccga aaatcgctgg agaaccgccg
    47761 taacatcctt gtaccgcgtc accacccagg catgcagata gggatcccag tgcacgggat
    47821 cttcgcgccg aagccggtga tatagaggat atgggttagc caaaacgccg ggatccagcc
    47881 gctgatacag gctgagcgcc gcatcctgac cgtccatggc cgggctcgtg cttccgctca
    47941 tgactttgcc tccgcgcagc accctggcgt cccggtggga gcccctgcgg atcggattcg
    48001 cttctcttct gccaatcggt cgagttccgc agacagagct gctaccgtgg gcgcgtcgaa
    48061 caggctgcgt agtgaaattt cgactccgaa aatcccccgc aattgggcga tcatctgcgc
    48121 tccgagtaaa gaatgccctc ccagaagaaa aaaattatca tttctgccga tggcatcgag
    48181 cttcaaaagc tcggctagaa tcccggctac acatacttct gttggcgtcc ggggcaggat
    48241 ggatgcctgg tcttgaagcg tattgccgga gtccggcggc ggcagagcgg agtgatcgat
    48301 tttgccgtga gccgtcagcg gaaatgcagc gagccgtaca aacagcgcgg gcagcatgta
    48361 ctcgggcagt tggccgcgaa ggaaatcgcg cagctcggtg tgtgtcacgc gggaggtttc
    48421 gtccagaatg acatacgcga tgagccgctt ttctccgcct tcatcctcgc gagcaacaac
    48481 caggctgtcc cgaacctccg gccgccggtt gagcgcgctc accacttcct ttggctcgat
    48541 gcggcagccg cggatcttaa tctgatcgtc cgcccttcca agaaacgcga gctggccgtc
    48601 aggcagcagg ctcgcaagat ctcccgtttt gaacagccgg ctgccaggct cggggtggaa
    48661 gggattcggg acgaattttt ctttggtcaa ctccgggcgg ttgcgatatc cgcgcgccag
    48721 gctcggccct ccaatgcaaa tttcgccggg aaaccctgcc gggacgggca aggcgcgctc
    48781 atcgaggagg tagatttctg tgttgtcgat ggcccgcccg atagggggca gcgtgtctgg
    48841 gtattcgcca ggaccgagac gagtggaagt cgcgacaacc gtgcactcgg tcgggccata
    48901 gttgttgaag acggcaaagg ggagattcgc cggcggatag tgatggagcg tatcgcctcc
    48961 ggtgagcaaa atgcggaggg aaacgctggc cggccagtcc agcatgaaaa tctgttccgc
    49021 tatgggtgta gggataaaac tgatcgtcac ttttttggcc agcagccact cttgcaaaag
    49081 ctccgccgag cttcgcacag cctcatcggc aagatggacg ctggcgccgc tggccagata
    49141 cggccaaagc tcccacaccg cggcatcgaa tccgagtccc gccagatggc tggctcggtc
    49201 cgctgcggtc accgcaaagg cccgccggtg ccaagtgacc agattgctca agccggcgtg
    49261 cgtgatttcg acgcctttcg gcgtacccgt ggagccggac gtatagataa cataggccag
    49321 ttcgtgcggc attatttccg agaagagcgg atcggcccgg ccatcgcctg ccagcagcga
    49381 agccgcatcg actacggctc cagaccatgc agcgaatcgc tgtatgaaat gctgattcgt
    49441 caccagaacc ggcgcctgcg catcttccag catgaaggca agacgctcgg cggggtatcc
    49501 gggatccatg ggaacgtaag cgccgccagc tctccagatg cccagcgcag ccaccgccat
    49561 atccaggcaa cgcggcaaac acagaccgac aaggtcgccc ggttcgacgc ccaaagcctg
    49621 caactgcgcg gcaaaggcgc ccgctcgccg atgcagctca aggtatgtca agacctcggc
    49681 gcccgacgca acggcgatcg catcagggcg ctcggctgcc tgcccggcca ccatttccgg
    49741 gacgagcctc gcttcacggg ccgcaggcgc ggcagccgtg cacagagccg atttttccgg
    49801 agaagtcgtc atgtcttcac ctcgtcaatt cacaagaagt gtgcgatgca gcctcatctg
    49861 gaaacaggcg aagagcgccg tatcgacact ggtcagcttc ccaggtccag tatcggaacg
    49921 gacgccgggt cgtcccggcc gcgagtgcgg cagcgcagcc tttatgcgcg gatatgtcat
    49981 gtacacaaat gtgatgtaca caccggtccg gtgcataatc gggcgacccg gcactcgtaa
    50041 agctcagaag gcgctcgggc cctgggtaca gaaatttctc caaagtcctg atcccatttt
    50101 cgattcccca tccacaggcc ttcgtacagg cctccatgcg ggtccaggcc ttaaagaacg
    50161 tttgtagtct cctgtgggga gcgagagcat ccagctctcg agccgcctcc ggcgaaagga
    50221 agctgcgcgc cacagtccgg tcgattccat cgcgaatctg ctccacgtcg atgcccacgg
    50281 gccctgcgcc gctgatggcg aatacggcga cttgtccgga atgcgagacg ctaaaatgca
    50341 aacttccccg gccgagctcg ggcttgccgc gttcgccgta gcgaaacatt acgccgggcg
    50401 gcggcactcc cagataccgg ccgagaattt ctctcagcgc cgcccgggca atgatgtacc
    50461 gcctgcggtg gcgcagcagc gggaatagcg cggctttccg ccgttcctcg acatcgagca
    50521 ttcgcgccag ggcgcccaga gagccgccgt gttggtccag atttgcccgc cacacatgaa
    50581 tttcgccaac gttcagcttg agggggcccc gaggcgccgc ccagatcaca ccgcctcgct
    50641 gccgcctcga caatcgcgga gaatctaaga ccatacattc tcctcggaat actccggagc
    50701 gggggcgggg ctaggttcat agtcaaacgg cggcgcggga caccggcggg cctgcgccca
    50761 acgccatagc tcccgcagat cggcccacaa catcagcagc acgggcgccg ggtcatcggc
    50821 ggcgcaaaac gccgcctcgc ggattccaga gagcgatttc acccactgcc gcagcgtcag
    50881 cttgccatcc agcgcataac gaagactagc ggccaaatcg aggtcttcca cgatccactt
    50941 gcgcccctcg gccgctgctt gtggaggaac gggacgtccg gttaggtgca gatacaaggc
    51001 tcgggctaca tccaagtcgt cgcgcgacac aaataagcgg aacgtgctgc cgattcgcgg
    51061 gttgacatcc agcaatttaa attcgccggc gcgagcgtca tagctgaaat caatgtcgac
    51121 aatgccccga aaaccgaggg attggaccaa ccgcttcgca atcctggcga tagtctcgtt
    51181 tttttgagtc aaacccaggc tcgtaacgcc ggtgtaagcc ggacattgac gtagttttcg
    51241 gccggtgaat cctgcaaggc agtgggaatg ctcgtcgaaa tatccattga acatccagtc
    51301 ttcgccatcg gaaatatatt cctgcacgac cacgtttttg accgcgccct cctccatgcg
    51361 ggcgcaaaga tccagcagtt gctttgcgct tcgaatgacg aattttgttt tgctattctg
    51421 cctccttagc aatacgccgg agcccttgat catcaccgga cggccgcagg attcgaaata
    51481 atgaagcaga tcggcttttg tcctagggta ttcggttcgg gggatggaga tattgaggcg
    51541 ttgggcgacg tgatacactt cccttttgtc acaaagagag tggatcagtg cggggctgac
    51601 ctctggaaag agatacgact gcctgagtat ttccgcgttc tcggccacaa atacagttgc
    51661 ggtgtcggtt gtgggaatca aaatataggg ccggccggcc cgcttcggta ttcgcatcaa
    51721 aaattcgacc gatttagccg gcgcggtgct atcaatatcc cagataaact tgccacggct
    51781 gtatcgggag aaaaacgcag gggtcgcacg cgcagcgtcg acgttgaaga caggaattcc
    51841 cagacggccg aagctacgtg tgatcccgag gccgccatgg tggggcgagg ccagaatcaa
    51901 cacaggcgtt gaaggatcgg tgatgatcat cggcatttgc ccaggcaggt gattcacacc
    51961 ccgtctccag cgtgctgctg ctggctcgaa ctactcggga gggatttagt cacaggtgaa
    52021 tggggccgtc gccatccccg cggttcagat gcatgttgga aagcgctgga tctaaatcgc
    52081 tcagcaaccc ggaaagctgc ttggacatga gcagaaagaa attggacggc cccacaaaaa
    52141 acccggcctt tttcaggccg tcgcaccaag ccggatggct ctggttcgaa accaacaaat
    52201 cgaccccgcg atgctccaga ttgttcgccg cggcggctac tacctgcggc gcatcttcgg
    52261 gcaacgccag acaatccaca atagagccca cgcgcatatt gccgaaatat ttgttgcagg
    52321 acatgaccgt atccagcaag accgcccatc cgatggcgcg tgaagcggcg caaattttca
    52381 agcggatgaa cctgggatcg ctggccggat aaaggatgtt caagacctcg gaatctcgaa
    52441 ccgcagtcat cgaatagcgg ctgcggcaca tcctccagat ttcgtctgtc caggatgcga
    52501 actcagaaac ctcttccgct aagggcaagc cctggcgggc ggtcttggct ttgaggagca
    52561 accgcactgt ccgcgctccc agccagccca tacccgaata ggcggctgca tccagtgcga
    52621 gccgtctggg caatccggtt ttcaggtatt caatgttccg caagaaccgg aagccgttca
    52681 gcaccttgaa gaagaaagga acaactctcc aagtccagcc catggcgcga agcatacggc
    52741 tgatgttggc gtcgaggctt ccgatcccga gacagtacaa cagaggcatt ctccgcagcg
    52801 cgtcatggat gattttcgga cccaccaacc cataccgttt gtcggcaatt gcctcagaaa
    52861 tgggcatccg gcaggcggcg gccggcaaaa gacggcccgc gaaagagaac tcctggcgct
    52921 tgagcgtgta ggctcctcgc acaaagccat cctgcacaac aacaaaaatt tcctcgtaga
    52981 tctgtctgtc acccactttc ggcaaccagt caggagctgg cctgacgggc agcaaaaatg
    53041 gcgcgccggc gcggcggatt cgctcgttga attccgtgac cgcccctacg agatcctcgg
    53101 tatagggcag gactcgcaga gcctcgctgt tgacctggct cgaagaccga ttgccagcag
    53161 ccggtccgcc attttccaat gccaatcctc ttccgtgttt ttccaacggt aaatggccca
    53221 ttaccacaga ggtccacgcg tcagccgaat gtgatggctc catcccgtcc ccttttgccg
    53281 cgcgccgggc tcggcctgat ccgccccgcg tgcgccgcac tccgcctgca acgaaagtac
    53341 caaatcgccg atagttacta acagtattgg aaaccggtcg ccgcagagag cattgcatcg
    53401 ccggcgcccc cggaaacctt cttgccggga gcagaaagct tccacactga agagaatctc
    53461 cactccgcag agagcgccgc gctttcatat ttatgaacgg cccgaatccg gcgattaagg
    53521 tgaaagccgg agagcgctaa ggcgctttcc caataggaag tagtacgcat agcggttaca
    53581 gtaacccgtt gggcaacgca agagattctt gccgttgact ggccacgcat tggtctcccg
    53641 caccgccgct cctcttcctc tgctcacgag ctaaggcggc aaagttagtt cggctcgtgc
    53701 tccttttact gtgcggctcc agcgtggagg gtgtgccttt ttttgaaggc tatgcgccac
    53761 aggaacgtgt ctacgctccc ggatgatccg acgacgcccc aagttcctct ccccccagat
    53821 caacgccgga atcagcccga caaatccact ttgtgaggta aagagtgaag ccttcagttt
    53881 cacgagtgac cggtttagca atagctgtcc cctctttgtt gttttgttgg ctgctactgc
    53941 ccggactggc cggcgcgcag ccggtagtgt gggtggcttc cgacatggtt cgggtgggcg
    54001 catcggatgc gccgggttcc ggaacgcaag cgcagctttg ggccgggcgc ggcgagtacg
    54061 aagcctttca aatcatcatc cgggccccga gcggaggatt gagtaacgtc aatgtcaccg
    54121 tttctgacct gagcggtccc ggtggtcaaa cgattcctcg cagcagttac acgctctatc
    54181 gcgagaagta tgtgtacgtc agcccgggca gccctaactg gggcggctcg aaccagccgc
    54241 tcggagcggg ctggtaccca gacgctctga tccctttcac cgacccggca accggagcgc
    54301 cgctgagcgg ggcctcgctg caagccgttc cgttcaatct ggcaggcaat acaaaccagc
    54361 caatctgggt tgatctcctg gtgccgcgca ccgccccggc gggccagtat aacggcacgt
    54421 tcaccgtcac cagctcgcag ggcagcgtga gcggctcggt agtcctgaac gtatggaact
    54481 tcacattgcc gctgaccccg tccctgcact ccagctttct gtattggacg gcgggcagcc
    54541 tggcagcgca agaagaattg ctccggcaca agatctcgcc tttggccacc agcccgtcca
    54601 accagagcct gctgatgaac agctacgggc tgggcagcac ggggctgccg ttctggagcg
    54661 gcgcggacgt gagcacgtgc agtatgtcgc cggctccgtc ggtgagccaa ttccaggctt
    54721 cggcctccgc gcagcagccc ggattgctgc tctacgatta cagcgccgac gagatcggca
    54781 attgctcgaa cttgatcact acaatcaagc agtgggctta caacatgcat caggccggag
    54841 tgaaaaacct cattacgatg tctcccaacc cgcagcttta cgacgacggg tcgggcacgg
    54901 gccgctcggc agtggacatc tggaccatgc tgcccatcat gtatgaccaa aatgtcagcg
    54961 cggtcaacta tgtgctccag aaaggcgacg cggcctggtc gtacaacgct ctggttcagg
    55021 actcgtattc gccgaaatgg ctcatagatt ttgcgccgat gaacttccgc atccagccgg
    55081 gcttcatcag ccaagccttg ggactcaccg gcatgctcta ttggcgcatc gacgactggt
    55141 cgtcggatcc ctggaaccag gtgaataaca ccggcacgtt cagctcgaac aactaccctg
    55201 gggagggaat gctggtgtac ccgggccagc aggtcggcat caacggcgtg gtcgcttcca
    55261 tgcgcctcaa gtatctccgc gaaggcgtcg aagattacga gtacgtgcag atgctgaaga
    55321 acctgggtca gggaacctgg gctatgaacc tggtgaagac catcgcctcg agctggtcga
    55381 attggaccag agacccgaac gccctggcct cggttcgcat gcaactgggg cagcagctcc
    55441 actctctttc cggaggctct ccaaccagtt cgggcggcgg ctccagcgga ggaactacgg
    55501 gcggcacagc gcccgcggcg ccggccagcc cttcgcctgc taacggtgcg acaggcgtca
    55561 gcaccagccc gactcttacg tggtctccca gttcgggggc cgcttcctat gacgtatact
    55621 tcggaacagc ctcgaatcct ccgttggccg ccactgtcgc gacggcgagc tatgcgccgg
    55681 gaactctcag cagcgggact acatatttct ggaaggtggt agccaaaaac tccgtcggca
    55741 cggctgcctc ctcggtctgg tcgtttacga cctcggcccc tgtggtcagc agttcgacgc
    55801 cggtcgcagt ttcggtaact ccttcctccg gctcgggaat gagcgcggtc tttagtctgg
    55861 tgtattctga cgccagcggg gccgcggaca tagccggcgc tggcgctttg atcaatgcca
    55921 cgttcaatgg gcaggccgct tgctggtttt actacgatcg cgccagcaac cagatctcgc
    55981 tcgccagcga cgatacgact acttggacgg caatgacact cggctcgggg aattcactgt
    56041 ccaacagcca atgcagcatc agcggcagcg gatccagcgc cgtcgcatcc ggaagtaacc
    56101 tgacgctcaa tgtggcggtg accttccagt ccgctttcgc cggctcgaaa acgacgtatc
    56161 tatatgtcca gaacaaggcg ggtcagtcga acggctacca ggcaaaggga acatggacag
    56221 tttcgtccgc ccccgtgcct cccgtagccg tctccgtttc tccctcgtcg ggatccggat
    56281 ttagcagcgt ctttcaattt atctattccg acggcaacgg cgcttccgat atcgccggcg
    56341 cgggggcctt gatcaacggt agcttcaacg ggcaaggcgc ttgctggttt tactacaacc
    56401 gcgccagcaa tcagatctct ctcgcaagcg acagcacggc aagctggagc gccatcaccg
    56461 tgggatccgg tggagtattg aaaaacagcc agtgtacgat cgccggtgtt tccgtcagtg
    56521 cagccgcgag caatttgacc gttactgttc ccatcacgtt tcaagcgaat tttgcgggat
    56581 ccaaggcggt ctacatgtac gtgcaagata aagagggcca atccaacaat tatcaaaaca
    56641 aggggaactg gatagtccca taggctaagc gctttccagc cgacgcgcgg ccgcgcctcg
    56701 caggggcggc cgcgccctgc tgcgcctgga gttgctggat cttatctccg agctgtctgc
    56761 gcgctgcttc gagcgcattt ttatcgcggc cccagttttt ccagtcaggc cctacggact
    56821 tcgctacctg aagggcccaa tcgccttgcc ctaatctctt gaggatctcg acgtactcgt
    56881 aatcctcgct gccttcccgt agttgcttca gccggatgga gggaaccacg ccggagacgc
    56941 ccgcgggctg ccccgggtac accagcattc cttcgccggg gtaattgctg gaagtaaaga
    57001 cgccttcgtt attgacctgc gcccacgggt cggatgacca tttgtccacg cgccaataca
    57061 gaagaccggt aaggccgagg ctttgactga taaaaccggc ctggatacgg tagttgatcg
    57121 gatcgtaatc cagaagccac ttcggggaat acgaatcttg cgccagggcg ttgtaggacc
    57181 acacttcgtc tcctttcgca agcgcttctg cgatccgttg cggggcgcta tcgtacgttt
    57241 tgggcagcac gacccagata tccacggcgg agcggcccgt ccccgagccg tcgtcgtaaa
    57301 gggccggatc cggggccatc gtaatcaaat tgcgcacgcc ggcctgatgc aggttgtttc
    57361 cccactgccg aatcatcgga tagaggttcg tgcaggctcc gatttcatcc gccgtatagt
    57421 tgaacaggtt gaggtcggga gcgtgcgctg aggcagccgc ctgaagagcg agcggggaag
    57481 gcggcggccg catattgcaa tgggaggcat cggcgccgct ccagaaacca agatccgtac
    57541 tggtcaaacc gaagttggca atcagggttt gctcgagcgc cgccaaacct gtgcacggat
    57601 tcgctccgca actgcccaat cgctgcggca tgatccggtg tttgatcaat tcctcccggg
    57661 cctcgttggt cgtggcgctc caaagcgcaa agctcgattt tagcgacggc ttcgccggca
    57721 gctcgaaatt ccacacggtc agcttgcaag gaatggccgc ggaaccttga tcggccgtga
    57781 ccgtcaccgt gcccgaataa tcgccagcga ccgcgtcacg tggaacgtaa atatcgatcc
    57841 agaacggctg gtttttccct ccatcaatgt tgacaggcgc cgccggatac gcaccgccgg
    57901 cgggcgggga gcccgttttg ggatccaaaa aagggaccag cgcatccgga taccagcccg
    57961 gcccgagagg ctggttcgat cccttcgcat tcgggcttcc ccggtccaca tagacatagt
    58021 gttcccgata cagcgtggcg ttattgctgg ctatgactcg gccatcggcg gttgtcaggt
    58081 cagatatcga cacgctcacg ccagtcaagc cggatggcgg cgcctggaca acgacttgaa
    58141 aaggctcata ctccgctttg gcggaaacta atgcggctga cgcgttgggc ccgggcgcat
    58201 ccgccagccc gatcctgtct aacccagtgg cgacccaaac cgtgagagtg gacacagttt
    58261 gttccgtggc ttcaggaacc gctacggcga tgctgagagc gatcaaccgc aatatcaatc
    58321 tgcccagagg aaggctatag ttgatcattg cttcctccta tgcgaggagg ccggcgcctc
    58381 ataggggctc ctgtttcggg gggcgcgagt ggatcggccc aggaccgaac ttgtttgcgc
    58441 cgaaagtttt ctccggagct ttcacttgag aggaaagcgt ttctttccat ctttcagctc
    58501 agattttgca gccgcggttc atcttccaga tgcagattca ccggacccgg cgcgcctgtt
    58561 ttgccggtga gagcggctcc ttcagggccg ctcgatggat tcatacagag ctcgctgaga
    58621 gccggtgaag gggcaagcgt ttccgtatac agagcagggg ctgatatgcc gttccccttc
    58681 tcctgcgcca tgcgagccgc gagttccgcc atggtgaggg tttcaatgtc acccgactcc
    58741 cgcgccttta atatccgctc gagaatgcct tcaaagacgg aaaacccctt cggcgattcg
    58801 gccagattca ccggatgcag acagaaatga aacactccat tatggcggat agcggcatcc
    58861 agcccccgcg ctacgcgctc aattcggctg cggagaacaa ccagacggct gcgcgactcg
    58921 ccgattggat aaagaaacag cgatgccggg atattccaca ggcccggcag cgtctctttg
    58981 ggccatacgg gtggaggcgt cgcccggcct aattcctcca gtatccgcag caaggagccg
    59041 ggaagcgttc tgcccagctt ttcggaaagc acgggcgggc gaccgcggta acaaagaatg
    59101 ccgttagacg ccagaagacc gtggcaagct tcctgattcc gcgggaaaga gaacgagacg
    59161 ggccgcaccc ggactgcctc caaagcctcg atgccaccga aaaattcccg gcggattact
    59221 tccggcttcg accgccaatc ggtccaaatc aaatgcgtca agccgccgtg caacccgacg
    59281 tcctgtttgg ggcgagcctc tgccaaacgc ttgacaaagg aacgccgata ccagagcgcg
    59341 gcgattcctt catcccccgg cggaatggag cgagtccagc gggcgggcag gcctaagagc
    59401 ctgggatctc gcgggccgtc gctttcggca agcgccatac cgcccaccac cagccacgtc
    59461 gcgctcacgc cagccgagca aaggacgccc aaaaggcgat cgtaagcggc cagagcgctc
    59521 ggataccgtt cgcagaactg ctgctcattc agaagatcga gatgtcccca tatctgctct
    59581 gcatcgagcg aaatgatcgc gacagcgcgt ttcaggcttc tgtgaaaatt ctcatcggga
    59641 gctggcacga tttcacccca tttctgttac cgagaaatag aaacggccgg cccgcgcggc
    59701 ccaaaccggc cgtccacatc gagaaaagga cgttttgcct ccgtcctgga gccggcgaag
    59761 gccgccatga cccgggagtc ggtttcatct gcctgggctg atactccagc gacctcaaga
    59821 aacttctgat tgctgtaata gaagtcgcga tagtctgtca cctcacccgc ttccaacgcg
    59881 ctcttgagtt cgcgaatccc gtcttccagc gaccactggg accggaatcc aagctggttt
    59941 tgtatcttcg tgaacgacac gcgatagttt ctatgatccg cgttatcgat gttttctacg
    60001 caaatgcccg gaaactcatg acgaattttt tccgcgactt gcgacaacgt aaaattcaaa
    60061 cgcggatcgc cgacgttaaa gatttgaccg ctgacgactc cgagcggggc ggtcattaca
    60121 tgttcaacag ccggcgccac gtcccgcaca tgaagaaatg gccgccactg ctggccgttg
    60181 tagatggtga tcactttttc ctggtaagcc ttggctgtca gcagatttac taccagatca
    60241 aatcggggcc gataggagtg cccgaagacg gtcgacaggc gcagaattac aggatgaaag
    60301 gactcgctgt gcgcttccaa cagcgcttct tccgaattga ccttcgtccg cgcatacagc
    60361 gacaccggat tcacagtcgc ccgctcatcc accagctctt cactgattcc atacacactg
    60421 caactggaag cgaaaatgaa tcgcccgata caatagcctt tggccacctc aattaacatc
    60481 ctggtggcgg catagttgac ctcgagagcc gtctggcggt cttgttcgca agcgggatca
    60541 ccgacgatgg ccgccaggtg aacaatggca tgaactccct ttacagctcc gacgactgtt
    60601 tgaatattgc ggcaatcgcc aacgaccagc tggagattgg gatggttcaa aatatcccgg
    60661 atcgacgcat acccatagac gaggctatcc agcaaccgca cgttgtagcc ccgatcgagc
    60721 agccgccggg tgagtattga tccgatgtat cccgctcctc cgacgattag aacactggcc
    60781 ggatcggcaa gccgacgcgg cgcagttttc tccacggcac gccgcgtccg gcggaaggcg
    60841 tcttgggccg cacgagctaa agtcacggca ataatgacca gagccgcaaa ccaggcggcc
    60901 agactctcag aaaccagacc ttcctgaaag accaagtaat tcgccaaaag ataaaacaac
    60961 acagccaggc cggcgccctt gacaatagct gtccgccgat accgaataaa atagaatcct
    61021 ggcttctgat aaaagccgct ggccataaag accgcaggaa agataaggga aagcggccag
    61081 aagcaatgcc aataatattg cggaaattcg gttgcgatgt tgtgcgcggt ttgttggtcg
    61141 cccagaatca cgtgaaatgt aacggcaaac gcaagagcca tcgccatgca cacgtgaacg
    61201 aggataaaat ccagcagaca ggccaagcta atcttcccta gcgacattct cggttctccg
    61261 ggtatagcgt tgcgggctga ttgtcaggag gcagcggcat acccgccgcg cggcggaagg
    61321 ccgcggaggg agcgcccatt cgaaattgct ctccggacgg ctacgattac gtccgccaca
    61381 tcgctgtcgg acatttttgg atagagcgga agagaaagca gttcattgta gattttctcg
    61441 gctataggaa aggacgcctc cgagcagtgc agcgctctac ggtagtaggg gtgccggtgc
    61501 aggggggtga aatgcacgct cgttccgatg ttcgcttgtc tgagcttttc gatcaacaaa
    61561 tcgcggccgc cagccatgga atccacgcgt aatggatata gatgatatgt atgccttcgg
    61621 ccgggcgctt ccgcgggcaa gcgaattccc gggacgtcgg ccaggccggc cgcgtaactt
    61681 tctgcaatct cccggcggcg ctcgatgaaa ccatccagcc gttgcaactg atgaattccc
    61741 aaagcggcct ggatatcggt catgttgtac ttgtaacccg cttcgtgaac ctcgtaatac
    61801 cacgacccag gctgcgtata gcgtttccac gcatcgcggc tcattccatg cagggagagc
    61861 aggcggattc tgccagccag ccagtcgtcg tttgtggtga gcatgccgcc ttcgccggtg
    61921 gtcatgtttt tcgtcgcata aaagctgaaa gcgactgcct ggccgtgggc gcctatcttc
    61981 ttcccgccgt actcggcccc gaccgcatgg gcggcatcct ccaggatcag aacgtcgtgt
    62041 ttttttccca aatcgagcag agcgtcgaga tcgcaggcct ggccggcgta gtgaacaggt
    62101 atgatggcgc gggtgcggcc gctcaggctg cggcgaatag cgtccaggct tatgtgaaaa
    62161 ttgtcgtcca cgtccgccag aaccggcgtc gccccgagat gggcgacgac gttcgccgtc
    62221 gcgcagaacg tgagtgtagg cacgatgact tcatcgcccg ggccgatgcc aaaggcggcc
    62281 agcgcgacgt gcagcgcagc cgtgcaggag ctgagcgcga tggcgtgctt taccccgacg
    62341 tattgggcga aatcctgttc aaacttcttc accttgggtc cggtggtgac ccagccgcgc
    62401 tgcaagctgt ccacaacttc ctgaatttcc tcgggcccga taaaaggctg gcaatacggc
    62461 agaaatgcgc tgcgcataaa acgtgatccg gttaaccggt ttgtgaaaaa aagcgagcct
    62521 ggctaaacga gctttcggaa agtaagactg gcgccgcaca ggcggaagcc gcacttttca
    62581 tacaagcgtc cggctgccag gtttcgcaat tgagtcccga cctcgacaat gcgaacccct
    62641 ttctcgcgaa accatcggag cgctcccagg gtcgccaggc ttgccactcc ctggccgcgc
    62701 gcgttttccg cggtcgctac catcacgatc gtcccaaacg acacgccgat cgtggaagcg
    62761 gcttcctggt cgatcttgca ggtgacgtag cccaggacgc tctcgccctg ggaggcaatg
    62821 attacagcat cggctgcgct gcccaggcac gagtttcgca gccaggcctc gttaatgtga
    62881 tcggcggcgg ccgagctcag agcgtagtcg gcatggaacc ggtcataaat ataggccgtc
    62941 ctggcgattt tcagcacctg gtcgagatcg gattcgcgga aaagacgcac gcaaacgccg
    63001 ctgggggttg cggcgggcgg agcttccaga tccagtccat ccagtgaaat cgcgaacgtc
    63061 tggatgccgt cgatcaactc gaagcccgac gcctccaggg catgaatggc cgaaaagtct
    63121 ccggtatcca cgcgagccgt taagtgccgc acgccctccc gctggcactg tttgtaaaca
    63181 tcgccgatca agcggcgctt gacgtgtcgg ctgtcccagt aatcgccctc cgcgactagg
    63241 aactcgagac gggcgcttgg gaacccgaac aactcgctgt cccagagcag cttgctccag
    63301 caagccgcgc cgcataggga ctggccgctc cattgcaaca gcagagaagc ttgttcgctc
    63361 gacagtaagc gctccaccct tttttgagag agccgttgaa aggcttccgc tggtgccaga
    63421 tcttggtaga cacggcggcg agccaggttt tccacaatga agcgcaccgc tacctctgat
    63481 tccggaagaa ccgcgaggga gttactcatg ggagtgcacc ggctgatttt cagcaagcga
    63541 ttcgggctgt gccgagacgt agatgccgcg ccggctgacc acgtacggcg ccgtcttcag
    63601 cagaagctta acgtcgagca gcatcgagcg cttctcgacg tactccacat ccagttgttt
    63661 gcgctgttgc cacgggatgg aattgcggcc gttgagctga gcgagacccg taatgccagg
    63721 cttgacgtcc aggcgccgct tctcggcggg agtataaaag ctgaactgat ccgggagttc
    63781 cggcctcgga ccgaccaggc tcagatcacc cgcgagcacg ttgatcaact ggggcaactc
    63841 atccaggctt gccttgcgca gaaatcgccc caccgcggtc agccgcggat cccggtcgcc
    63901 gttgaaagtg gagccgtccg cgttgcgtag atcggcgcac ccggcgcgca tggtgcgaaa
    63961 tttataaatg gtgaagggaa cgccgtagcg tcccaaccgg cgctgccgga agagcgctgg
    64021 ccccggagac gtcagccgga tcgccgccgc aatcaagaac atcaggggac tcagcagcag
    64081 cagcagcaag cccgaaatcg cgacatccaa aacgcgcttc gccgccggat acaactttgc
    64141 tttatgcaac gcggggtttt tggttcgcgt ttccagttct ctgacggctg cgcagtactc
    64201 gtctgcatat ttttcgagca tggtttgctc cgtatacgac gcaagataga cactgcgagc
    64261 ggtgcggccc aagcgttcgg ccaagctcgg gtcagcgatc agcttctgca tggcctctgc
    64321 cagcgcaccg tcgtcgcccg gcggtacaag cagcgccgca gagccgtttc gggtcgcctc
    64381 acagttgctg gagatggccg tagccacgat cggtttgccg gcagccatgg cttccaatag
    64441 tgctatcgaa aggccttccc gcacgctcgg aaggaccacc aggtcgctcg ccgccagcag
    64501 atctccgaca tcgttccgga atccgagaaa ccgcactcgc ggcgcaatgc ccagttctac
    64561 tgccagcgcg gcgataccct cccggatcgg gccgctgccg gccaggcaca cgctcagagg
    64621 caggccgcgg ctgattaaca ccgaggcggc tcgcagcaga tcctcaattc ctttcagcgg
    64681 cgccaggcgc cctgtcgaaa gtacaactac ctccttagct cccgcctcca agctcgctct
    64741 taccgcatcc ctgccggcag cggcttcgac tctcgattgg ggaattccat taggtatagc
    64801 cctgatctga tgaccgccgg caatgcgcaa tcgcaggccc caccggcggt gaaactcgct
    64861 gacagtgaag atccgtcggc accagcggga ggccgcgcgc tccaaaacgg aacaggctgc
    64921 aatgacgagc cgcgacgagc gctcgtgaaa ggcgaaaccg tgggcggtgt gaaagatgat
    64981 aggaactccg gcgagccgcg ccgccagtcg tccgacaaag ccgcccttgg aggtgtgcgt
    65041 gtgcacaatg gtgtagcgcg ccgttttgag aaagcgatat aggcgaagca agcccacaac
    65101 gtcgcggaca ggacggatgc tgcgccagat tacatcgagc gccacaaccc cgattcccgc
    65161 ctcgcgcagc gcggcctgaa acgtggcatc ggtggtcaag acatcgacat cccagcccat
    65221 gctttttgcg tgacgggcca gttggaggat aatgatggaa ccgccgccaa acttcgattc
    65281 gcccacaatg tgaaggactt tcatattccg gatcccgaac agccgccggc tgcggggcta
    65341 ttccacaaca tggtcgagta aatactccaa ttggggttct tgagagctgg cggcctctct
    65401 tacatttttc tgatccccgc cggctctgtc cggcttgcgg cttaacggat ccaggcgcat
    65461 ccaacgcagg attgcgtagg tggccgcggc ggcggccaac tgggagagcg tggccgccca
    65521 agccatgccc acatccatgt gccgcggggc cagcagcact gccaggccga tgttcagaac
    65581 tccggcgccc aacactatca agttgaaggg acggtccagg tcgagaggca gcatccagtg
    65641 aactcccaga acattgcgaa gcgcaactat gggaggaaga atggcaaaaa ctcgcaggac
    65701 gaccgtggcc ggctcaaaac ccttgccgag cagtatccgc acaagctgcg gcgcgaaggc
    65761 gaacatgaac agacccatca gcaggccgat cgacaccagc acgagagcgg ccagccgcgc
    65821 gaacctttgc gtttccggcc gagagcgctt agccagattg ctgatgcgcg ggtaggcggc
    65881 gtcgctgacc ggccccagca gccccagtat tgcccggcag attctgtcag cgccggcgta
    65941 gtagccgacc acgtggggag gcgcgaacaa acccaggata aatgcgttgc ccactgtgta
    66001 catgatcgag gcattccgcg gaacgaaagt ggtgatgctg cctcgcaatg cccccatcag
    66061 caggctgatt cgcggcagcc ggaaacgaag atcgcggtag acaaggccaa gcgacccggc
    66121 cagcgaaagc agcgacgctc cgccttgaat gccaagcacc ttccagccgt cggccggagc
    66181 tcgtaccaga aagaaaatcg ccgcgaccgc cgagctcttg gccaggagat cgatggtcac
    66241 cgccgctttc atgcgctcga tgccctggta aaaccacatc atgttgagac cctggaccag
    66301 cgaccaaaac agcccggccc agaacaaggc ggaatgctgg cgcagcggca aaacgaaatg
    66361 cctgactcca agggccatga ccaggcataa cgcgacgagc agcagcttcg cccccatcac
    66421 tccggccacc aactcggaga gcttgtcctt cgcttcctgg tggcgggcca cctcgcgcgt
    66481 cgccgaaaac gtaaagccaa actccacgat cagcaggaga tatccgccga aggcttgaac
    66541 gaaggcgact tccccccaac tcgacggccc cagtactcga ctcaggtagg gaaccgtaac
    66601 gagagcgagc agataacccg agcaatagag cccgtaaagc gcagctacgt tgtgcgccaa
    66661 accggaggga acacgaacgc tgagaaagcg ctttaccatg ctctccccgc acgtgcggct
    66721 gtcctcttcg caatcgcgct ggatgcgccg atcatcgcca tgatcagcca ccagtgcctt
    66781 tgataaagcg cctcgattcc ggctgacaat ccgagcatgc cggcgtgcgc tcccaacaag
    66841 cccacaatca gcggccgctc gtccgggccc atcgatcggt atgcggcggc tcctctagtg
    66901 aaaaaccaca acattagtcc taaaaatatg ccggccccgg cgagtccgaa ttcggtcatc
    66961 atccaagccg tggtgttatg aatgatgtac ggccttcgta gaacagtcgg gtctccctta
    67021 gtgaactggt caaattggcc caacccgatt ccgaacagag gactttgcga aaagaagggg
    67081 agagcgtcct gaatctgctg gaggcgctgg tgtgctgtat tgggacggcg cgccagaaac
    67141 acggcccgcg actggccgag caaaagcatc acagcaaatg cggctgcggc ccccatggca
    67201 gcataagcgg gcaacagcga aggccggaac gctgcgatca ccagcagcgc cgcggccagg
    67261 ccaatccaag cggaacgcga aaacgtcagc atgagcgccg tggccagcat cacgctggct
    67321 aaggctgcgg gagtcctgcc caacaacggg cgcggcgaaa acagcgtcac ggtattgggg
    67381 accagcgctg caacaagcaa gcctccaaag gcgtttggat cgatcaacat gcccgaaaca
    67441 cgagcggcgc cgtagttgat cctgggcacg gatacccccg agaagtacac aacgactccc
    67501 agcagcgcgt gcagggccgc cgcgactatg aagatccgaa gcagcgagcg tatggcggac
    67561 caacttccgc agttgctcgt aatgcataga tagctgccca tcagtgagag caggcccagg
    67621 tccttaatga ggatgtaaga agtcaacttg gcaagctgat tcagcgcgac aaaagtggat
    67681 atgaaaaata tgatcaccag cgccgcgtga aaaacactcc acgccccgcg gacaatgcgg
    67741 atcctcccca gctcgatcgc aaaacagagg gctagaaaca gatccgacgg agcgagattc
    67801 atggtgttgg attcgaattg cacaggaagc aaaaaaacat atgctccgag aaaaaggggc
    67861 aacaatcctc ttccatgccc cgttgcgggg ccaggggaag ggactacatg ccatcggggc
    67921 tcgccccgaa gcagcggcga ttgcacttcg ggcgtcatgg ccggctagag ttttcgcgta
    67981 gcgtaggttg catagccata aaaggaatcg gttctcggat cccagccgtt cagaattgtg
    68041 ccgagcacag taaggttatc cgccaagagc cgctggagag tggcgacggc cgcttcccgg
    68101 ctggtctttt ttgcgcggag aaccaaaatc accccgtccg ccgcacggct cagcaggcgg
    68161 gcgtcgggac catgcagggc tggcggggcg tctatgatca ccatatcgaa ctcctgccgg
    68221 agttgagcaa ccagttccgg aagccgctcg ctatgcagca gagcggagat gccagcggct
    68281 tctttcgtct ccggggcgat ggtgagcggt ccgctcggca gaagataaag atttggaatc
    68341 cctcgaacca tctccgcata tcgcacagct cgggtatcga gcacgctgga gtcgcagagc
    68401 agatcgctca gccccggccg gcccgccaaa ttgaaaattt tgtgcaagcg aggacgccga
    68461 atgtccccat cgactaaaag cacacggcgg ttcgtttggg ccaacgcgat cgccagattg
    68521 gcggccaccg ttgtttttcc ttcgcccggg ccgggactgg tcagggtgat aacgtgcgcc
    68581 gcgccctcgt accttatgca caaagaggcg gccgcatcgt gaaacgattc aacaagcagc
    68641 ggatatgcgc catcccgcgc agccagctct actttttgtc cgttcctatg cgggttggcc
    68701 ggcaagcaag cccgtaaacc ggcgaggcag ccgcgctgcg gcttcggaat cgcggctagt
    68761 tcaggcgtct gcaagatgga taaagcttgt cccggcgatt tgaacgttac atcgctgtgc
    68821 tcgcgcagta gaacgacgcc gaccccgaaa aacagcccgg caaagagcgc cacagaaagg
    68881 ttgagcagat agttgggttt gtagggccgc gccggcggca gggcgcgatt gagcactcgg
    68941 atgttgcttg ccggaaccgc ggctcccagg ctgagttcct gggccttttg cagcatggaa
    69001 gcatgcagcg cccgagccgt ctcaacttcg cgcttgaggg tgttgtaatg gatcgatttc
    69061 gccgccagac tcgaaaccag cttcgactgg tcggcgtatc gcgtggaaag caactgctcg
    69121 cgcctcaggg ctgcctggta ttcattttcg attcgtttgc gggtggcagc cagttcgcgg
    69181 tcgcgggccg cttccagcac ggcaatctgg ttttggacgg tgcgtacttt gtagtgcgac
    69241 ggggtcaaga gagactcgag cgattcaagc tcccgccgca gatcggtcag cttcacctcg
    69301 taatcccgca gcgcattgtc gcccaccacc tgaggaaggg attccggcgg gacgtccgcc
    69361 gcggcctgat actgcgactg cttcatgatt cggtcggcct gcgccgccgc cagttcattg
    69421 ccgagtaacc gcagcctggc ttctgcaacg ttgtcctgct gctgctctcc tgtgaaaagc
    69481 aaccctgcgt tctgcgcata atcctgggtt tgccgctcga gactttccag agttgtgcgt
    69541 atttccgcaa cctgtccgga aagccattct ccgacccggc gcgctgtttc tgagcgggcc
    69601 tgctgatgtt cacggataaa ctcgtcggcc aatgtgttag cgaattgcgc cgcaaaccgc
    69661 gcgtccggag aatcgtacgt gatatgaata agccgggcct gattccaaac acccacacgc
    69721 atccgcacca tcttgagcgc gcgctcgcga ggcgaaattg gcgccaccgg cagccccgcc
    69781 gcattccgga cgcgatccac caagccgggt gccgtgaatt cgggacgatt taagagattc
    69841 agacggtctg cgacccgccc cagcacggac tcattttgaa gaatttccac ttccgtttgc
    69901 atggccaggt cgcccgcgcc gtcgggcggc gccgccggag ccgccggctc ccgaaagggc
    69961 gtgactgctt cgttcagcgg ctggtactcc agcgatgtct gcgcttgata catgggcgtt
    70021 tgccccgccg tgtaccccca ggcgcccaaa acggcagcta aagcgcataa aaagatgaac
    70081 ggcgcttttc gccggatcag ccgccaataa gtgattaatg tgtccagccc ggcgtcactt
    70141 gacgcggtgg atggcgcacc gagcggccat acgctcgctt cgggacgcga gaggccgatg
    70201 ctgtcgtcat gcacggcaga cgaattcatg atgcggggct ccagggatcg atcccaacgg
    70261 tgtggcatat gctttcgaag tcgttatcgg ggcagaccca cgcgccagat cgtaatgccc
    70321 gtgcctacat tcaacgccgc ttccgccgcc cggagggcca catttctggt cgcgttattc
    70381 ggaacgaata agatgtcctc cgcctccatc atgatgtccc tggatttccc ggccaatatc
    70441 tgcttgatgt tgacagcaat ctccacgcgc ttttcgtcgg aggcgcttgt acgaagaatc
    70501 ttggccttct ctggggccgc gttcggtccg agtccctccg ccagagaaag ggcctgcaat
    70561 acggaaatat gctcgcgctc gctcaaggcg aagccgcccg atttgtgaac gtcgccgatc
    70621 acatacactg tttgcgcctg ggagaccgag atgacatcgt ccggttcgat cagtatgttg
    70681 gcgctgggat tggaaccttc catcagctct cgcaggctta cctcggccat actgaaacgc
    70741 ttggttggat cttgcaccgc ggtccggagc ggtatttgcc cgcggtctga ccgccgggtg
    70801 attcgcactg tattgcccgc gtcatttctg acgccctcgg ccatggagag gacctcgatg
    70861 agcgtcttcc gcccgcgaag ctgctgaatc cccggcttgt tgaccgcgcc aatcaccgac
    70921 acgggctggc tgtgaaaatc agtgaccgac accgtgacct ggggctcctt cacaaatacg
    70981 cgcaggcggc ttattatctc cgccttaatc tgctcgggtg tccggccaag cgccagaatt
    71041 ctgcctatca gcggtacatc caaatacccc tcggaatcca cgcggatggg tttggcggaa
    71101 atttcttccg agccgagcgc ccaaacgcta agttcgtcgc ctggcccgag catataagct
    71161 tctgcgttgc cggggtcgat cggactcgcc agcccgcccg cggcggcctg atgttggggc
    71221 cggccggaag gcgccgccag cagcatcata gccagcatca atgcgatgga gccttcagcc
    71281 ttcagaggcc acaggtcccg gaatagcttc gacggcataa aactaactcc acgagatttc
    71341 ccgctgccgt aaaaccagat cctcgttgat ctcaaccagc accgaacgtt gcagcagagt
    71401 aatggacaaa agcagccgga agggcctcgt agaatccacc acgacgccct ccaccccggc
    71461 cagcgggccg ctggcgatcc gcacgcgctc gcccgcagtt agaaaggcgt ggggctgcat
    71521 tggaaaactg gcgcgcgccg ccgattgcag cgcttttatc tcgttgtcgt ccactggggt
    71581 cggcacgcgc cccgcaccga ccagccggat gacgcccgga gtcatcagaa taggcagccg
    71641 caccagcgcg tcgaaccggc aaaatacata tccggaaaac aatgcaagct caaactggcg
    71701 gaggcgcctg ccataccggt gttgacgccg gtaaagcggc acaaacgttt cgtagccttt
    71761 gtaccgcaac agacgcgata ccgctttctc atggcgtggc ctgacggcca gcgcaaacca
    71821 ctccagtgcc tccgaatcat cgcgtagccc ggcgcttggg gtttcaggtt cgggccgcca
    71881 tctattcatc agtggagtaa gagggcgttg ggagtttcct ggaaacatag ctaccgcaat
    71941 ctcagttatc aggaactgag tccacgctgc tccctgacgt tcaagtccaa tgcaacgcca
    72001 atgccagtcg gcctcaccgc gtgggttgcc caggcaaatc aagcgctctc cgtatcgtta
    72061 cagagtccgg agaggcgcga tgacagcttt cgtggctccc ggcaaagtgg aaggccgggt
    72121 gacaactgtg gcatcgcggt tctctacggc ggccaagtgg agggctgcgc tgtatagtcg
    72181 tacaattttg catctttcgt ttcgatgcgt ttctggcaat tccagcgaag aatctcaatc
    72241 actttttttg aaaagaagat atgttcgtct atcttttttc aaaacccagg caagcgcctg
    72301 aggtgcgatt tctttgatat cgaggcggcg gcattgccac gccacagcgt tcaatcaggt
    72361 ttgaccagcc tgccagccgc acgaaattcc tttggtaaca aacggtgcgg cgcattcaga
    72421 tgcgggccgc ggacgtatgt acgtttacgg ttgcgtaacc ccaaaaatgc aaaatcgacg
    72481 agcgaatgtc ggcagcgtat gttagccctc tacttttttg gcggccgaac tctttatact
    72541 gcggtagtgt tgtttccagg gtagttgttt cgagcagcgc tgccgtttca attttaaggc
    72601 gcgctgggag tgccgaacca agcgtcaggc cattgcgtag cggagtggcc agagccggac
    72661 aatccggtag cacgctgatt ccactttcga gtggaatcgc ggcgcagata gatcttctta
    72721 tgaaggacac tatggcgcta gcagggacag aaggtgaagc ttcaagcgtg gcgcaaaccc
    72781 acttctccgc ggataggcaa cggaactgtg tgcggcgccc gcgcttcccg cgcccggtcg
    72841 cgccttcggc gtatctccag gaatcgggag tcgtgccgct ggtcatcgaa actcctgcaa
    72901 agtggcatcc ataccctcta cgcacaaaga aagcgctccc ggaggagtgg ggagattcac
    72961 ttcgcacgca agggactcag aagcccgtta tcaacatcga gccagccgct ggcgggcggg
    73021 aaagatttcg ctttttagcg cccaggctcc cgtcaaatac tgccagtcct gagggagtca
    73081 gcgaggcaat gcggattgaa ccaggttctt ctcccgatca cgtcaataga aacgcgagaa
    73141 acgtgtgggg cgcgggagac ggaactagtc ctgagttgcg acccggaagc gctcgcccca
    73201 gtggcccctg cgcgggaatc ccgcagtcga ttcgaacaat gcatagtcga gacatcacga
    73261 agaaaactgc gttcggcaaa ggcttcgtga cgcttgtaag tttgcccttt ctccagttga
    73321 ccagcgtgga ggctatggta cccaatgaac agacgccgag ttctcgtagt agatgacgac
    73381 aagagcatcc ttgcctatct ctccgatctt ttggccgatc gctacgacgt agactgcctg
    73441 gattcgggcg aaggcgtcgt gccctatttg ggctccggcg cccgcccgtc tttgatcctg
    73501 ctggatttga tgatgccggc ggtcagcggt ctggatgtgc tggccgcaat cgaacgcctg
    73561 agccgccggc cgcccgtgat tgttctgtcg gcgatcggcc aaatcaacac cgtagtgaga
    73621 gcgatgaagc ttggcgcatc ggattatctg gtcaagccgg tggaagaaca ggacctctat
    73681 caggcgatcg aaaacgcatt agaagagtcc gatgacccag aagaaacggc ggcgccggcc
    73741 gctgcctgcg gggccgacat catttctgcc aaccgccagg tgctccgtgt caagcaaatc
    73801 gccgcgcagg tagcggacgc agaggttcct gttctcttgc tcggagagtc cggggtgggc
    73861 aaagacgtat ttgcccgcta tatccatgcc cgatcgagcc gcagcgccgc gccgttcgtc
    73921 aaggtcaatt gtgcggcgct cccggcggac ttgctggaat cggagctgtt cgggtatgat
    73981 cgcggggcct ttacaggagc tcttcgggaa aagccgggga agtttgaact tgctggccgg
    74041 ggaacgcttt ttctggacga aatcggtgaa atgagccccg tgctccaggc taaactcctg
    74101 cacgttcttc aggacggaga atacagccgc ctcggaagca cacggagcac gctttctcaa
    74161 gcacgcatta tcgcttcgac caacaagcgc atcgaagagg cggtatcgaa gggcgagttc
    74221 cgcgaggatt tgtacttccg tctgaatgta attcgtctgc aaattcctcc ccttcgcgag
    74281 aggcgcgagg atattctgcc gttgtcgcat ttcttcttgg aaaaataccg ccctcaatac
    74341 ccgcggtcgc ctcggcagtt gcctcgcgag ctgatccagg ccattctgct gcattcctgg
    74401 cccggcaatg tgcggcagct tgaaaacacg attaaacgtt tcctcgtgct gcctgacctc
    74461 gattttgcgc tggccgaact gcgggaatct ggcactccgt ttaatgtcag ctcgccgccg
    74521 caaaatcgtt ccctgaagcg tgtatccgct ctggcggcag aaagagcaga aaaagaggtg
    74581 gtccttcgca cgctcgatga gacgaactgg aacagaaagc aggcagcccg cgagctcaat
    74641 atttgctata aagccctgct gaacaaactt cgaaaatggg atataccggg acggtccaaa
    74701 ggccccatcg agccttccgc gctctaaccg ctgccacggc gctccacgcg cggatgccgg
    74761 tcaacctgct caactggcca gagccgggag cgctcgccgc ggggcaatat tccagacaag
    74821 gttctgctcg cgcccagagg ggagtttcac ggccgcctga attcacagct ttgcggccgg
    74881 attatcaggc ccagatgttc gacacaaaaa cgagtttcac cggcttgaat cccagccgga
    74941 agtgactcgc gagcgaaagc tcattcccct gctccacata ggtcagcacc cgacgtcggc
    75001 cgagatcctc catcagagcg gcaagatggc tcttaaacct aagccatacg ccgctcccgc
    75061 ggtattcggg caatatgaac gcatcgtaag catagataca gtcgcatggg accgtaatac
    75121 ggtacttaaa cggctcggcg acgtggcgcg gcctgatgga gaaccactcg tatccaatga
    75181 tgccaccgcc aagttgggca acaacacaat gatcgccagc ctgaaagcgc tctaggaact
    75241 tctgatattt attttctccg cacattgcga ggcgattgag gtcattcagc gaccctgccc
    75301 gaatttctcc cgggccgcgc aacatggatg atggaatata gggcggatga tcgagcttca
    75361 gcaggtacag gcgtccgatt tttagacaat gaaacgagag ccggtcaaca agggcgcgga
    75421 gaatgcataa gaagggcttt cggcccagcc tcgctatttt ccgggaacgt aaggcggctc
    75481 tcgccacggc aatgcgctac acttcgggga ccggcgccac cagcacgggc tgccggtgca
    75541 gatagcgcgt ggcattcctc ttcgagtcga tcagttcgta gacccgctgt acctgtgccg
    75601 gcgagaggcc tgcttccgcc gccacttcct ccaccggagc gccgcagttt tttccgtaga
    75661 ggcagagatc cattttgtca taaggcaagg aaaagtaaaa ttcttcctgc gactgatgca
    75721 gtggataggt atccgtggtt ggattgcgcc gccggatttc ttccggtatg cccagatatt
    75781 cggcgagctg gtaaacctcg gacttataca gatgagcgat cggcttgaaa tcggccgcgc
    75841 cgtccccgtt tttgacgaaa aatccctggt catattccag ccggttgggt gtacctgcca
    75901 cggcgtaccc cagaaggtcc gcatagtagt attccatcat cttgcgcgtc cgctgcttga
    75961 agttggtggc ggccacgatc tgtgaatacg cctccgccgt gagccttgcc ttggtttgaa
    76021 cgtgcgacgg cgattccacg acgatcgagt acaccgcgta gcgcggtccg ccgagcaatt
    76081 caggcagaac gagtttacac ttatagccct cgccatactc gggaaccaca ctgcgaatgg
    76141 cctcgtcgcg gcgccggtag cagccggcgg cggcgaggat cggggtaatg tcctcgacga
    76201 cggatcgaat tcccagttga tcggcgacaa gcttgcctaa gcgaaggctg tcggcggacg
    76261 aatccgcctc gggaagaaac aaaccgagca cccggtccct tcccagtgcg cgcgcgcaaa
    76321 gggcggctac gacgctgctg tcaaccccgc cggagagcgc aacgacggcg cctttgcgtc
    76381 tcaaatcgcg ccgcacgatc tcgcgaatcg cgctctccag gcgcagggcg gcaccgccgg
    76441 catccatctt taaaagctcg gcggaaaacc tcgtagagat gttcatcgtt gttctccggc
    76501 tgaggaagcc aactcacgcc tgctgatctt gccgttggcg ctcttgggaa gcgcggcccg
    76561 aaattccaca aacttgggta tcatgaagtc ttccagatgc cgggagcaat ggcgcagaat
    76621 ctcctgctcg ctgatggcag atccgtcgcg caacgtcacc accgccttca cagcctcgcc
    76681 cagcaccgga tcgggcacac cgatcaccac agcctccgca ataccctcca ggccatataa
    76741 aacttcttcg acctccctcg ggctcacttt ctcccctttg gtcttgatga tgtcatcctt
    76801 gcgccctaca aagtagagat agccctcttc atccatttga aagagatctc cgctgtagag
    76861 cactcgctct cccggcattg gccccggctt cagcaccttt tcggtttcct cgggcaggcc
    76921 ccaatagccc ttcattacgt tggatccccg gattaccagt tctccaactg tgcccgggcc
    76981 gacccgctgg ccccgctcgt ctacgatgta aacttcttca ttcggtatgg cctttccgac
    77041 cgacttaggc cgaatgtcga tctgatcggg cggaagatac gaaacccgtt tgcactctgt
    77101 gagcccgtac atcgagtaaa tcttgacgtg cggaaaaatc ttccgtagct gcgcgatctg
    77161 gtgaaccggc agcgcagccg ccgtattcgt gatataacgc aaggccggaa aacgataagt
    77221 gctcagatcc atttgcagca gtatcgcggc catggtcggc accaaaggaa gccctgtcac
    77281 cttctctcga atcattgtct ccaacacagc gcgcggatat gtgaaggagc gctccaatac
    77341 aagcgtgccg ccgatctgga aagccatcag aacctgatac aagccatagt cgaaggatag
    77401 gggcagaacg tttaaaatca cgtcatcggg agtgttctcc aaataggtcg tgatggaggt
    77461 cgccgccgaa acaatgttca ggtgggtgag catcacgcct ttcggccttc ccgtggaccc
    77521 tgacgtgtaa ataagggcag ccaggtcgat gtcgatgcat tgcttctccg gaggggtgtc
    77581 cggcgaaacc gaatctccga caatacggtc ccattcggcg tccgccaaca cgattgattt
    77641 aaggtgggaa agcccagaga gctgctgctc gagcccggcg atccgggcgg aatcagtgat
    77701 cagcgcacaa gcccggcagt cgttcaaaat ataggccagc ttttcagcct ttgtgctcgg
    77761 atgaatgacg aggaaaatcg ccccggcctt gagaatcgcg aaaagggcca cgaccgcttc
    77821 cggcgagttt tccagttgaa cggcgacccg atcgccgcgg cgcacaccct gtcgaatcag
    77881 ggcatgcgca agccggttgg actggcgttc gatctcgatg taggcgagcc ggcgcgaccc
    77941 gcacacgagg gcagtctttt cgggcgattt ttcggcgctg cgctcgagaa aggtttccac
    78001 ctgcacgctg tccctcctac gccgccggtt ggagcttggt ttgaatgaat ctgctgaggt
    78061 tgttgatcga atccagattg tccggcacca gatcttcatc ttcaattttg atctgatatt
    78121 gccgctccaa caaactcacc agttccagaa cgctcgtgga gtcgataatg cctttttcca
    78181 aaaaagagtc gtcgtccgaa aatttgccgt cgtcttggcc gaaaagaaga ttttccacca
    78241 ccaaagagcg caattcctga ttgagtgttt gattaagtgt ttccatagcc ggatcttttg
    78301 ctgaaactcc tgatgaattg atcaatcaca agctgagtgg agaggatgcc gatgagcgcc
    78361 atgttatccc gaatgctccc ggagccgccc cagcgaaacc tgttgatgag cccttgcaca
    78421 ggcgcgggat tgaagatgcc gtcttgtttg agacggcccg gggagagaag ctcttccaca
    78481 taatcgtagg gggctccttc aaaaaaactc cttgcctcgg gcgctcgata aggctgtttt
    78541 gccctttgct taatggattt cggtattaag ccgttgacgc actccttcaa cagatatttt
    78601 tcattgaggc cccgcatctt cagcggcggc ggcagggcgg cggcgaactc caccacgcgg
    78661 tagtcgagga atggcatgcg gcactccaca ccatgagcca tagccacgcg atcgccctgc
    78721 gaagacagaa tatagccggg aagtaagtaa ctcgcttcga ggtactgcga ctgtgagaaa
    78781 ccatcccact cgaaataagc gggcggaagc agagcttcca aggctgcgta acaatcccga
    78841 gtcgccagcc cggatttgac ttgatcggaa aagaacaact tcaatcccgc cgtcagctcc
    78901 cagcgcggag cgtgcgagaa gaagggcttc tgcaactctt cagggcgaat ccggaaaaag
    78961 gcgcgcaggt aggcgttcga ttggcgttgc agttccggca gatacggata gagccgccgc
    79021 agtaacagcg gtctgattcg ggaatccgga tgggcggccc aaaaccggcg gatctttgcc
    79081 tctttgaaaa tatcgtaccc gcccagcatc tcgtcggagc cttccccggt caagaccacc
    79141 ttatagccgt tggacgccgc cagccgggaa agcaaataca tgggtgcggg cgccgtccgc
    79201 aaaacgggct gttccacatg ccacaccacc tcgggaaaca ccgcggcgat ttcccggccc
    79261 gtgcagcgga cttcctcatg atccgtctcg agaaagcgaa cggcttcccg ctgatacgcg
    79321 ctttcgtcga agcccggatc atcgaagctc accgaaaatg ttttcagccg ggagtcggaa
    79381 aatttccgga tcagcgccgt ggtaatcgtg gaatcaagcc cgccactgag gtaagcgccc
    79441 accggaacgt ccgatcgcag gcgaatcctg gtggcgtcta caagcagcgc aaacaagcgc
    79501 tcggcgctgt cccgctccgg gtgcggcgcg cagttgaact caagccgcca atacggccat
    79561 accctcacgt cacggccgtc cacagccatt gaatgacctg ggggcagttc ttggacattt
    79621 tgaaaaattg tccggggagg gactctgcac cagaaggtga aaatgtcgtc gagagcgtcc
    79681 aaatccagcg cgcggctgac gccgggatgg cccaaaacgg ccttgatctc agatccgaaa
    79741 acaaacgccc tgccggtaac cgtataaaag agaggccgga tgcccagtct gtcgcgggag
    79801 aggaagagac gctgtctccg ggcgtcccag attgcaaacg cccattggcc gttcatacgg
    79861 gagacgcaat cttcgccgta ttgccggtaa aggtgtagta tgacctcagt atcggattgt
    79921 gtacggaagc gatgaccttg ttcaagcagc tccgcgcgca gctccaggta gttgaaaatc
    79981 tcgccgttga aggtgatcga gagcgatcca tcctccgtct gcatgggctg gcatcctccg
    80041 gctatgtcaa tgatgcttaa tctggcgtga gcaaggcctg catgggactc gcaataaacg
    80101 gctgttgcgt ccggcccccg atgccggatg agacctatca tggtccgaag caatgcggga
    80161 tccgcccgtt cgtagaagtt caaaacaccc gcaatcccgc acattggctc acacgctcgc
    80221 ttccagccgg gctgagtttc tcccgcaagc ggatgctctt ccgccggttg cgggatcgtt
    80281 cctccactcc agcctggcgc tgtgtaggaa cgcacatcca atcgggcgcg gacgcccgaa
    80341 cgtgctgcga tgcacactcc gtgccatccc gccctgcgcc ggctccagga tgtgagaccg
    80401 atcaccggtt tcgctctttg aggccggccc cgcatgcgga tttcgccgga aagactgggg
    80461 gcgcgcgctc aactatttga gagcaggact gatcgaaaac tgggccgcct ggcggaatgc
    80521 cggttcaatg aagcctctgc tgcggaaaat ggccccaaag cctcaaaagt cccgccggca
    80581 gggaaaaact aaccaggaat atttgacggt aagttgtaca cgatctgggt gcggagagat
    80641 tgtgcgcgga aaagccgatg ggaggcgccc cggccaggaa aatttgtgcc agagcggacc
    80701 gcagaacccg acatccacga tccgctttgc acccctgcgt ccgcggcctg cctcaagccg
    80761 gagtcggcgt cgccgcgggt gctattttcc gctgccttcg ggcgcggtag cgctcaacgg
    80821 cgctcgacat gtacgtgtag atgacaggag tgagatacaa agtcaccaat tgcgagaaca
    80881 gcaggccacc gacgacggcc aagcccagcg gctggcgcgc ttcgccgccg gcgccgtatc
    80941 ctagcgcgat gggcacggtg cccagcaacg cggccatcgt tgtcatcata atcgggcgga
    81001 aacgaatcag gcagccttcg tagatcgcgt ccagcggctt cagaccttcg ttccgttcag
    81061 cttccaacgc aaaatcgatt tgcatgatcg cgttcttctc aacaataccg atcagcataa
    81121 tcaggcccac aaaggcatag atgttcaaat ccatgcggaa caggaacagc gtgagcagcg
    81181 cgccgaaacc ggcggacggc aagcccgaca ggatcgtgag cgggtggaca tagctttcat
    81241 agagaatgcc cagcacaatg taaaccaccg caatagcgac aatcagcagc acccaaaggt
    81301 tggtcagaga gctttggaag gcttttgcgg ctccctgaaa ggacgtgctg accgtggccg
    81361 gcaaactgtg ctcggcaagc tcctgaatat ggctgacggc atccccgagc gaaacgccgg
    81421 gccttaaatt gaatgaaatc gtcaccgccg ggagctggcc gaaatggttg atcgtctgag
    81481 gcccggattc ccggttcacc cgggctaccg tatcgagcgg gatcaattgc ccgccggtcg
    81541 acttgaaata gagcagcgag agcgcgttag gatcagcctg atatatcggc ttaagctcga
    81601 gcaggacctt gtactcgttg atggcggcgt agattgtcga cacccaccgg gggccgtagg
    81661 cgtcatagag cgcgttctcg atctgctcgg cgctcacctg gagggccgcc gcccggtcgc
    81721 gatcgatgct gacgttcacc tgcggcgtcc ggatttgcag attgctggtg acgtcctgga
    81781 gctccggcag ttgagcgatc tcgcgctcca gcttttccgc tgccgaatac agctctgctt
    81841 tgtccggagc atgcagcgac aactgatagg ggctctgcgt cacttgtccg ccgagctgca
    81901 ccgtcggcgg gttctgcaag aagacctgca tgcccgggaa agccgaaagc ttcggccgca
    81961 gctcctcgat cacgtcattc accagaagct ttcgctgcga gcgtggcttc aagtgaacca
    82021 ccagttcgcc gaaattcggg ccgcccagcg tcgaagccgt ggtgccgcct acgctcgaaa
    82081 ccagagactc cacattcggg tcttcgcgga ccacctcggc gatggctttc tggtactcca
    82141 ccatctgata atacgaagtc ccttgcgctg cgccagtgac gatgacaagc tgatcggtgt
    82201 cttggtcagg aatgaagccc ttcggtattt tcagaaacat gtagcccgtg gccacaagca
    82261 cgattacgaa cgtagtgagc gtcgcaggcc ggtggcgcag caccgattgc aaagttacgt
    82321 cgtaaacgcg aagcatgccc tcgaagaacc gctcggtgac ccgatagaac cacgaccgct
    82381 gttgctcgtg gggcggccgc agaaagcggc tgcacagcat gggcgtcaga gtcaccgaaa
    82441 ccaaacccga aatcagaatg gccacgcaga tggtgactgc gaattcgcgg aaaagccggc
    82501 ccagcacgcc gcccaaaaac agcacgggaa tgaacactgc tgccagcgaa aaagtcatgg
    82561 aaacgatcgt gaatccgatt tcccgcgacc ctcgcaccga cgccagcatg ggatcctcgc
    82621 ccatttccat gtggcggacg atgttttcca acatcacgat cgcgtcatcc acgacgaagc
    82681 cgatggagag aatcagcgcc atcatggaaa ggttatcgag gctgtaattc agcagataca
    82741 tcacggcgaa ggtgccgatg atggaaaaag gcagcgcgag actgggaatc accgtagccg
    82801 aaatattccg caagaacaga aaaatcacca tgacaaccag ccccagcgtc agagccatgg
    82861 tgaactgcac gtccttgtac gattcgcgga tcgtgtcgga ccggtcatac aaaatattca
    82921 tcttcaccga cggcggcagc tccgactgaa aaatcgggag cagcgctttg atgccgtcgg
    82981 tcaccgaaat cgtattggtt ccgggctggc gctggatggc cagaacaatc gatcgctgcg
    83041 tcccttcatg ggtcgaaaac cacgaggcgc ttttatcgtc ctctacgctg tcgatcacgc
    83101 ggcctagttc ttccaggcgc acgggcgtac ccttgcgata ggccacgacc aggggccggt
    83161 acgccgcggc gctcgtcaac tggcccgtgg cctgaagggt gaatgcccgc tgcggaccga
    83221 taatggctcc cgtgggcaga ttcacattcc agttcttcaa ggcggcctcg acctcgttga
    83281 tgccgatctg gcgggcagcg agcgcgtgcg ggtcgatctg cacgtgaacc gcgtactttt
    83341 gggctcccag tacctggacc tgcgccacgc cgctcaccat cgagatgcgc tgggcgatgc
    83401 gcgtttccgc gtattcgtcg agcgtccaaa ggggaagagc gcctgaagtc agggcgatgt
    83461 aaagaatggg ctgatccgcc ggattcactt tggtgaaggt cggcggggcc ggcatgccgg
    83521 gcggcagcag ccgggcggct tgcgtgatag cggcctgcgc gtcaaccgcc gctccgtcca
    83581 agctccggct cagatcgaat tcaagggtga
//
LOCUS       DASREK010000016        20195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_999355, whole genome shotgun sequence.
ACCESSION   DASREK010000016 DASREK010000000
VERSION     DASREK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 20195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20195
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999355"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..518)
                     /locus_tag="VFA54_01310"
     CDS             complement(81..518)
                     /locus_tag="VFA54_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49471.1"
                     /translation="MPEVATPNQAEREFETGTDESFKNTSATAGAAHSENQRVTFANI
                     KRTYDVYQDLDIQAARQALVEQTRVNQIASQALQNAVETANLVSKQAVRHGDIAIDGQ
                     WNPIQQGAGDTLTARAVSIDDVSLKAIGAVVAAAVADALANRK"
     gene            complement(849..1181)
                     /locus_tag="VFA54_01315"
     CDS             complement(849..1181)
                     /locus_tag="VFA54_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49472.1"
                     /translation="MCKNIRIENPLTGAGFTSRNRAKRFVARGLAEWIEFGQSIRFLG
                     DPRDHREVAARKQVDATRYWYERAAHTGMAQLTELANLPMVTPHVLLGMGRRKGASRH
                     TFLVTQGV"
     gene            complement(1600..1677)
                     /locus_tag="VFA54_01320"
     tRNA            complement(1600..1677)
                     /locus_tag="VFA54_01320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1640..1642),aa:Pro,seq:ggg)
     gene            complement(2205..3284)
                     /locus_tag="VFA54_01325"
     CDS             complement(2205..3284)
                     /locus_tag="VFA54_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS49473.1"
                     /translation="MPLIELEGVSKYYTDSICALKDISFTVEPGEWLAIMGPSGSGKT
                     TLLNLLGCLDRPTAGRVRIGGVDIAALSRADLTRFRAETAGFIFQQYHLIPYLTALEN
                     VMVAQYFHSMTDEAEALKALERVGLRDRARHLPSQLSGGEQQRVSIARALINQPKIIL
                     ADEPTGNLDAVNEEIVLQILGRLHEEGNTILMVTHDEKVGRRADRRMDLEHGRIVQTT
                     VFSFEENENFDEVLEHMWMAEEGTPHLEAHHYTDAERKRLIATMTRIGLLEMDASGSR
                     VTFTAEGRDRAKSVIRRHRLAERLFTDTLSIRDDREIEQNACTFEHILSPEVTERICA
                     FLGHPTRCPHGSPIPPGPCCRRERP"
     gene            complement(3284..4369)
                     /locus_tag="VFA54_01330"
     CDS             complement(3284..4369)
                     /locus_tag="VFA54_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZS49474.1"
                     /translation="MIFWRMVWRAMTERRQRVALTLAALTITAALATALLDLYSDIEG
                     KLRGEFHGYGANLVIAPGAGRQTLLLEALAVAEKYGEAAPFLYSVETVNGEPVVLAGV
                     DFQRAEPLIGYWEVTGRRRPLPGECLAGERVAARFHLRPGSTLNIRTRLGDERRRVAG
                     IVSTGASEDSQVFLAFEEAASAAGLPHQTSLISVRAPGERADQVQAALAAALPEAEVR
                     LLRAVVESEAAVVLKIRGTLFLLTVLILVIAALCVMNNFSAIVHQRRKEIGILKALGG
                     ADRPIAALFASEALGIGAAGSVAGFAFGWVLARWLGWRIFHQPVAAHVSVLPAVAGIT
                     LAVALAGIIAPLRRIRRIEPAVILRGE"
     gene            complement(4470..5711)
                     /locus_tag="VFA54_01335"
     CDS             complement(4470..5711)
                     /locus_tag="VFA54_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS49475.1"
                     /translation="MRSPRRKLSTIAAVALASAIATAMLAVLVDVGDRVSRELRSFGA
                     NLVVTPKSTALPVEIGGVDYQPVADATFIPEEALPRLKSTFWRNNITAFAPFLSATVE
                     MGGGKVAVRGTWFRRSYTTATREVLSTGVRDLNPTWRVRGQWIDDPAPDPSAREALVG
                     SLLARRLHVDPGGTLKLWGEPFVVRGVLVTGGDEENEIFTRLETVQHFTGRPGQVSRI
                     QVGALTKPDDAFARKDPVSMTPQEYDRWYCSPYISSIARQIEEQLPMAVARPIRQVAE
                     NEGRIMNQVQLLMLLVSLAAMITAALMIWSAMATTVLERRPEVAVMKAVGAQNGLIAA
                     LFAVEVGVQGLLGGVLGVGLGIILARRVSLEMFHSALALSPLLPALVIVAAVAVSLAG
                     SAIPMRSALRFEIAPVLKEEQ"
     gene            complement(5789..7060)
                     /locus_tag="VFA54_01340"
     CDS             complement(5789..7060)
                     /locus_tag="VFA54_01340"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HZS49476.1"
                     /translation="MFASFIIALREGVEAALVIAIALSYLRKVGREDLHRVVYRALGA
                     AILASVAGAYLFRKLEWSEEAFEGWTLLASAAFVFTMVVWMWRHSKGLKAAMERRLGE
                     ISSRSSGAAAGVFLFVFLMVFREGVETVLLLSAVSLTSDSLSEAAGLVLGLALAVVFG
                     VTFIRGTVRIDLRRFFRITTVILFVVVLQLLITGLHELSEGGILPSSAREMAWIGPIV
                     KNEVFFFVTVLALAAWMVLFDWRRREEAAAPAGGAARRKAVWSARRERLWMMAVCSAS
                     FLFIVFVTAEFVYARAESQLTPAIPLAAVDGKVRIPVRSVSDGLLHRFSYTSAQGVAM
                     RLIVIQKPDQSLVAALDACMICGNQGYYQRGSNVLCRNCASAIFIPSIGASGGCNPIA
                     VRSHVEGSDLVIEAADLEPGARRFVKPSAGG"
     gene            complement(7408..7557)
                     /locus_tag="VFA54_01345"
     CDS             complement(7408..7557)
                     /locus_tag="VFA54_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49477.1"
                     /translation="MRGLSARAPYFHSGSAATLLDVVNFYDRRFGIGFTEQEKADLVA
                     FLGAL"
     gene            complement(7666..7815)
                     /locus_tag="VFA54_01350"
     CDS             complement(7666..7815)
                     /locus_tag="VFA54_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49478.1"
                     /translation="MDDILGVSVLRGSCGACHDTPNVGNHSFPAPLNIGAGDLTSLWM
                     SATYR"
     gene            7969..8145
                     /locus_tag="VFA54_01355"
     CDS             7969..8145
                     /locus_tag="VFA54_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49479.1"
                     /translation="MKRRRKRIIDSNEKRLRGKRDRPAGGAVALQISRVEIEGLSYRE
                     LDLEIDDGPELFGL"
     gene            complement(8312..8977)
                     /locus_tag="VFA54_01360"
     CDS             complement(8312..8977)
                     /locus_tag="VFA54_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS49480.1"
                     /translation="MAKIRILLTDDHTLFRQGIKTLLSAEADLDIVGEAANGTEAVEK
                     VSELRPDLVLMDIGMPGLSSFEATRQIKKVRPDTKVLFLTMYDDEDYLVQCMEVGASG
                     YVLKDSPAQQLLAAIRDVYRGGSYLSPRMLSQLVDDFRSRIKSAHRQPRFATLTTREK
                     EILKFLAEGESVKEIASGLNLSVKTVEAHKFNLMRKLDIHNKAHLVQYAIQKKIIKIP
                     VGP"
     gene            complement(9004..10605)
                     /locus_tag="VFA54_01365"
     CDS             complement(9004..10605)
                     /locus_tag="VFA54_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZS49481.1"
                     /translation="MRGLERTPVLPEDAVVLSDQVQDYIRRLAERIAPCSQQIENRWR
                     RRLARSGLRGNDPRLRALAAINPGAAADLLAAGNLADFFERVEYNGRRLAKLDVPPTH
                     VISSLREYEDALLPDLRRFFPKDYSNYLWSLDHLYFCILLTLNTAYFQVRDLEAQAFF
                     DLFQDELESLSVDDLLNRVLDSLMRTFNAQGGVVLLLLPQQNELMVKACRGLDPALAR
                     HFNVRVGEGFAGRIAETGKPQVVLDARADPLIRSSEVRRAIRSLWGVPLIVKGKVTGV
                     LHLDFTREYNCLPREMKLLEAVAERCALALEKAQLMETLAQREEQIRQLGEHMLKVEE
                     EERRRISRELHDEVGQAMLVVRLYLEMLQQEMTRHVPHLATKVEDALKVIDGTIRDMR
                     RLISALSPNVLEQLGLAAALRQFTNNFGRNFPIHVRLKLSRLGQLPQDTEIMLYRLVQ
                     ECFTNVIKHSKAENVLLAVNRSNGRLHVHVEDDGIGFDLEQVAHKRESFGISGMRERV
                     ALLGGKMEIKTRQMKGTKIDIQIPI"
     gene            10814..12460
                     /locus_tag="VFA54_01370"
     CDS             10814..12460
                     /locus_tag="VFA54_01370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZS49482.1"
                     /translation="MPLGGLGEFGMNIMALRCDDDILVIDAGMMFPGSELLGVDIVVP
                     DLTYLKENRQHIRALILTHGHEDHIGAVPFLLGELDTPVYGTPFTLALVERRLDEYDL
                     PRKPRLHPVKPKQIIEIGRFSIEFIHVTHSMVDSTALAIATPLGVVIHTGDFKIDPSP
                     TDNELFDLHTLADYGRRGVLLLLSDSTNAESPGFTPSERAIRGRMEDIFMRAQGSLIV
                     SCFSSSTHRVQQIIDLSYEYNRKIALVGRSMISTAEIAHDLGFLKIPDGLLIRPAEID
                     RLPRSQRTVLIAGSQGEPMSALSRVSVDKHKHVSVAPGDTVVLSSRVIPGNEKAIFRM
                     IDHLSRRGAEVIYGSTTPPVHVSGHASQEELNLMLNLVRPRFFVPIHGEYRQLARHAA
                     LAEHLRYAGLEESFVLESGNVLEIDSHGARKAGSVAVGRVCIDSDAVDEVVEDVIIHD
                     RRHLSEEGIVLTVIAINRHTGEIESQPEIVTRGFVAPEDGAELVSRAREIVISTLENS
                     TGEEKTDYGVMKAKISADLKRFIARETAKRPLILPVILEV"
     gene            12783..14264
                     /locus_tag="VFA54_01375"
     CDS             12783..14264
                     /locus_tag="VFA54_01375"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZS49483.1"
                     /translation="MQATQMESLNYVDGEWRSSRSSDLYEVQNPATLETLARVPLAGP
                     EEVDAAVAAAASAFPTWRRMPPQDRIQYLFRFRDLLIQHADDIARMITRENGKTLAES
                     RGELQRGIENVEVACGIPTLMQGYNLEDVASGIDEIMIRQPLGVTVAITPFNFPAMIP
                     LWFLPYAIACGNTFVLKPSERVPLTARLLFELLHITGLPKGVVNLVVGAKPAVDALLR
                     HPQVKAVSFVGSTPVAKYIYAEAAAHGKRVQCQGGAKNHVIVMPDADLAMTAKIVSDS
                     AFGCAGQRCLAVSTAVTVGDASKSFPSALLELASSIRVGNGLYEGVQMGPVISPASKA
                     RILNLIEKGIHDGGKLLLDGRNPNLDNGERGNFLRPTVIAGLGGQSALTDTEIFGPVL
                     TLREVSSLDDAIEILSRSPYGNAASIFTSSGHTARKFRYEAPTGNVGINIGVAAPMAY
                     FPFSGWKDSFLGVLHGQGRDAVEFYTDKKVVIERWPKEWSRKF"
     gene            complement(14307..16097)
                     /locus_tag="VFA54_01380"
     CDS             complement(14307..16097)
                     /locus_tag="VFA54_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZS49484.1"
                     /translation="MFRVCAAMALSSYVRGIWLFGGVLAFVPLLLSQGMGGGRMTARP
                     SPRARLDLPSPTVEFRDVAAEAGLTAPNVYGGVKSKKYILEMTGNGAAVFDFDNDGRP
                     DIFLVNGTRLDGLPAGEKPTSRLYHNDGNGKFRDVTRNSGLERSGWGQGVCAGDFDND
                     GLTDLFVTYYGHNSLYRNLGGGRFEDVTAKLGLPAAGQRWNTGCAFLDYDRDGWLDVF
                     VASYLDFDLEKAALPGASPFCTWKGLRVFCGPRGYPAGRNFLYHNDRGRAFTDVTQKA
                     GIAFSELHYNLGVVTADFDDDGWPDIYVACDSSPSILYRNNRNGSFTDVAVGRGAAYG
                     PDGQEQGSMGVAAGDYDNDGRIDLVKTNFMDETPTLYHNEGDAYFEDQTYLSGLGVNT
                     KVVGWGVNFIDVDQDGWKDIFMANGHIYPELAGARVGEDFRQPKALYWNLRNGAFRDI
                     TRSAGAALSAPAVSRGVASGDLDGDGALEIVVVNMNERPTVLKNFGPKGNAILIGLTG
                     TKSNRSAVGARVTVTAGELRQTDEVRSGGSYASQSDFRLHFGLGGAAKVDDIEIRWPS
                     GILEKLSNISANRSIQVREGEGIVKSDPFR"
     gene            16365..19649
                     /locus_tag="VFA54_01385"
     CDS             16365..19649
                     /locus_tag="VFA54_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS49485.1"
                     /translation="MLSLRQKTARCLSALLVVLGGAALAFGQETTGGIVGTVKDSSGS
                     VITGAVVTSVNIETDVQSQTTTDERGNYEFPLLRAGRYRLSVEAPGFQRLVRPEVIVN
                     TTERVRLDLTLQVGAVSETVTVTAETPLLQSEKATVGQVVEQRTIESIPLATRNFTQI
                     LGTSAGVVGSIFNADTPGTGSDSVSVNGSRRGSNNLLVDGVQTTNTLNNAPDGDGTPS
                     IEFLGEFKVMTSLYSAEYGRNSGSIINVTTRSGTNQFHGAAYEFLRNTDLNSRPFFNP
                     ARGENIQNQFGANIGGPIKHDKIFFWGGWESMRQHNANSALGSQGSSNGGVVITVVPT
                     LAQRQGDFSAFPKAIKDPTTGQAFLNNIIPQSRLNLISLNIQNAFVPLPNFSSGNNNF
                     FAAAPITTNLDQGAGRVDYRLSSSDTLYGRWFESMEFDLTPFGQGMPGFTQGSTRAKH
                     TGGFSETHIFSPAWVLESRVGIDMTDQYLSFGNNTDPTTLGLQPIKGVTLIAGLPKIN
                     ISNYVNGGFGNSSRWHDNIKTFTTSSTMTWVRSQHTMKFGAESVTSLLHPFNDQASRG
                     TWSFTGLATGNEYADFLLSQPSTKQFAAGPGELLMREQVWSGFFDDSWKATQNLTIEL
                     GLRYEAHFNPAAYNLHMVSFWPDRYQGVGSVDAAGIVQGGVTPGVPNDVVFGQWHDFG
                     PRIGIAYRIGDKWVIRTGAGIYYDQRTGQIAQGTFSNPPTFKNVTINCAVAGQVCSLT
                     QPDNWTFVDPGYDPKAIPFPTKTTDQTLTLSAIDPRAKTDRVIQDNFTIQRQLPKNTL
                     LEAGYVGTKGTYLATSYNVNPLMPQPDGTLVRRFPGFGSINYRNDMGSSTYHSLQMTL
                     KRRVATSTFQLSYTASKVLTNGNESARYSTALFAAPWNDWSRAKGPANFDRPQRLAFT
                     WVQDLPSKATSRLGRFFLNNWSVNGFFIAQSGFPLTVINTTSGQGLGGTTSNDNGSFF
                     ANVVAGAPLIHPTGSTKDSLQSYINKAAWIPAPAGTYGNSGRGMFRGPGQWNVDASAF
                     KDFSITERWKMEFRTECFNVLNHANFALGTDTSTSLNMNSSTFGQISGTSVNARLIQF
                     ALRLSF"
     gene            19684..19974
                     /locus_tag="VFA54_01390"
     CDS             19684..19974
                     /locus_tag="VFA54_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZS49486.1"
                     /translation="MDSPARRLLVHLFQSRALENRMFLLRVNYSAPHNNGGSAIFDFE
                     GSIQDELDQREGVLVGNLNLTALRKIRNAWNKVYGPENRYPEAYRRLRQESR"
BASE COUNT         4040 a         6325 c         5998 g         3832 t
ORIGIN      
        1 atcttacctg tgggtgaaaa gcggggcggc cccgcgcaag gagtgcggaa ccgccccttc
       61 atgcgccctg aggagaaaga ctacttgcgg ttggcgagcg cgtcggccac ggcggcggcg
      121 accaccgcgc cgatcgcttt cagcgacacg tcgtcgatcg acacggccct cgccgtgagc
      181 gtatcgcccg cgccttgctg aatgggattc cattggccgt cgatggcgat gtcaccgtgg
      241 cgcacggcct gcttcgacac gaggttcgcg gtctcgacgg cgttctggag cgcctgcgag
      301 gcgatctggt tcacgcgcgt ctgctcgacg agcgcctggc gcgcggcctg gatgtcgagg
      361 tcctggtaga cgtcgtaggt gcgtttgatg ttggcgaacg ttacgcgctg gttctcgctg
      421 tgcgcggccc cggccgtagc gctcgtgttc ttgaacgatt cgtccgtccc ggtctcgaac
      481 tcgcgttcgg cctggttcgg cgtagcaact tcaggcatgg ttgttggttc tcccttcgaa
      541 ggttggttgt ggatcagaac ggtttgcccg tcatggcgtg aacgggctga gatctcagtg
      601 cgtgaggctc tttagccctg gcagcctgtc aaccacggcg aacggctcgg gtgacggcgt
      661 aacagattca gtaacaatca cttgcatcac gccgcgtcaa ctcgtgtcag cctgtttttt
      721 gaccgtggcg ttagcaacag cgtggactcc acacacccgc cgccgcccgg ccgttccgag
      781 gacccggcgc tcgatcgcat ctacggaatt tccgtagatg cgccgcccct ccgccagacg
      841 ggtgcatctc agactccctg cgtcaccagg aacgtatgcc ggcttgctcc cttccgtcgg
      901 cccatgccca gcaacacgtg cggcgtgacc attggcaagt tcgccagttc cgtgagctgc
      961 gccatgccgg tatgggctgc gcgctcgtac cagtagcggg tcgcatccac ctgctttctc
     1021 gctgcaacct cgcgatggtc tcgcgggtca cccaggaagc ggatcgattg gccgaactca
     1081 atccactcgg ccagaccacg cgcgacaaag cgcttcgcac gatttctcga ggtgaatccc
     1141 gcaccagtga gcgggttttc gattcggatg ttcttgcaca cgggtactgt cgagccagcg
     1201 atggggttgc gggtatgccc gcaaagggaa ctgcggatcg ggggcgcggg tttgtttcga
     1261 gaggccccgc tcgcctctgg gggaacgctt cgcgagcgtt cttctctctg atatcagcgg
     1321 ggaccgtcag ttgtcaacgg tcgacgcgca agcaatcggg ccgtggcagc aggacgatcc
     1381 ttgaacgccg ttctcgtgta taggggaagg cgatttttct tctccctgag gagcagaatc
     1441 gaaaaacacg catgacatgg ccatcatgac cagccggacg gcgggaaggg caggcgaacg
     1501 agcgaggcct ggagaccaaa aatggactcc aaaacgccac caaacggact ccaggcgagc
     1561 aacggagtca cgcaagtggt tgtaactctt tgaaacgttt ggtcgggccg ccgggatttg
     1621 aacccgggac ctcttgcacc ccaagcaagc gcgctagcca ggctgcgcca cggcccgatt
     1681 ttccaggttt ttccatctta acatgacacc cgcccgatgc cgcccgctgg tggcgtcctc
     1741 tcagtgacct gccattgatg ccgatcggat gcaactgctc gggcaagcac cgccatgggc
     1801 tacagaggct ccgctgcagg cagtgccaaa tccgcgagac attaccggcc tgctctgccg
     1861 aggctgccaa gtcagcaacg ccatgttcgg acaccattcc ttcattgcgt gatgccctga
     1921 aagtaggttg atgtgacgat ggggtctttg cgttgacgga aagcggcctg agcgacaaag
     1981 accttccgcc gatttaagaa tttgggattc cattcccctt tggggacagg cagcccgcaa
     2041 cgccgaatca gaacactcct gcttcctgag ctgagatcgc ggaaaaaaac ggagtagctc
     2101 gccggcccca cgggccccaa gctgttgcgg aagccggcgg ccgactcggt ccgagctact
     2161 gccagaaatt aatcctcaat actcgaggag caacaacggg ccgatcacgg tcgttcacgc
     2221 cggcaacagg gccccggcgg gatggggctg ccgtgcgggc accgcgtggg atgtcccaga
     2281 aacgcgcaga tgcgttcggt cacctccggg ctgaggatgt gctcgaaggt gcaggcgttc
     2341 tgttcgattt cccggtcgtc ccggatcgag agagtatcgg tgaagaggcg ctcggcaagg
     2401 cggtgacgcc ggattacgct ttttgcgcga tcacggccct ccgcggtgaa ggtcacgcgg
     2461 ctgccggaag cgtccatttc gagaaggccg atgcgcgtca tcgtagcgat caggcgcttg
     2521 cgctcagcgt cggtgtaatg atgcgcttcc agatgtggcg tgccttcctc ggccatccac
     2581 atgtgttcca gcacctcgtc gaaattttca ttctcctcaa agctgaatac ggtggtctgc
     2641 acgatgcggc cgtgctccag gtccatgcgg cggtccgccc gcctccctac tttttcatca
     2701 tgggtcacca tcaagatcgt gttgccctcc tcgtgcagcc ggcccaggat ctgcaaaaca
     2761 atttcttcat tcactgcgtc gagattccct gtcggctcgt cggccaggat aatcttcggc
     2821 tgattgataa gtgcgcgggc gattgaaacg cgctgctgct cgccgccgga aagttgcgac
     2881 ggcaagtgcc tcgcgcggtc ccgcaggccg acccgttcga gcgctttcaa cgcttccgct
     2941 tcgtcggtca tgctgtgaaa atactgggcg accatcacgt tttcgagggc cgtcaggtag
     3001 gggatcagat gatattgctg gaaaatgaat ccggcggttt ccgcccggaa gcgggtcaga
     3061 tcggcgcggc tcaatgcggc gatatcgacg ccgccgatgc gcaccctgcc ggccgtaggc
     3121 cggtccagac aaccgagcag attgagcagc gtggttttgc cggaacccga aggccccata
     3181 atggccagcc actcgcccgg ctcgacagtg aaggaaatgt ctttcagcgc gcagatcgag
     3241 tctgtgtaat acttagagac gccctccagc tctatcagcg gcatcactcc ccccgcaaaa
     3301 tgacggccgg ctcgatcctg cggatgcgcc gcagcggagc gatgatccca gccagcgcaa
     3361 ccgccagcgt gatccccgcc accgccggca gaacgctcac gtgagccgcc acaggctgat
     3421 gaaaaatgcg ccagcccagc cagcgcgcca gcacccaacc gaaggcgaaa ccggctacgc
     3481 tgcccgccgc accgatgccc agtgcttcgg aagcaaacaa agcggcgatc ggccggtcgg
     3541 cgccgccgag cgctttcaga atcccgattt ctttgcgccg ctggtgcacg attgcgctga
     3601 agttgttcat cacgcaaagc gcggcgatca ctaaaatcag cacggtcagc aggaacaacg
     3661 tgccccggat cttgagcacc acggcagcct ccgactcgac tacggcccgc agcagccgca
     3721 cttcggcttc gggcagagcc gccgccaggg cggcttgcac ctgatcggct ctctctcccg
     3781 gcgcgcggac gctgatgagg ctcgtctgat gcggcagccc ggcggccgat gccgcctcct
     3841 cgaaggcgag aaacacttga gaatcttcgc tggcgccggt ggagacgatg cccgccacgc
     3901 gccgccgctc atcccccagg cgcgtgcgga tgttgagggt cgatcccggc cgcagatgga
     3961 atctggcggc cacgcgctcg cctgccaggc actcgccagg aagcggcctc cgccgcccgg
     4021 taacctccca gtagccgatg agaggttcgg cgcgctggaa gtctacgccg gccaacacta
     4081 caggctctcc attaacggtt tccacggaat acaggaaagg ggccgcttca ccgtatttct
     4141 ccgctaccgc cagcgcttcc agcagtaacg tctgacgccc ggcgccgggc gcgataacca
     4201 gattcgcacc gtatccgtga aattcgccgc gcagctttcc ttcaatgtcg gaatacaggt
     4261 caagcaaagc cgtagccagc gccgcggtaa tggtcagggc cgccagcgtg agagcgacgc
     4321 gctgccggcg ctcggtcatg gcgcgccaga ccatccgcca aaaaatcaac gttcttcctc
     4381 cgtgacggca acaggattcc gcccctcacc gcgaggcggc agccggagag tcccgggaga
     4441 gctagccgct cgcccggccg ctttggactt cattgttctt ccttgagcac gggggcaatt
     4501 tcgaaacgca gcgcggatcg catcggaatc gcagaaccgg ccagtgagac cgccacagca
     4561 gcgacaatca caagggcggg cagcaatggc gagagagcta gcgccgagtg aaacatttcc
     4621 aaagacaccc gccgcgccaa aatgatcccc agccccactc ccagcactcc gccgagcagt
     4681 ccctggacac ccacctctac ggcaaacaaa gcggcgatca ggccgttctg cgcgccgacc
     4741 gctttcatca ccgcgacttc cggccggcgc tcgagcactg ttgtcgccat cgcgctccag
     4801 atcatcagcg ctgcggtgat catggcggcg aggcttacga gcagcatcag cagttggacc
     4861 tgattcatta tgcggccctc attctcggcg acctggcgaa tcggccgcgc gacagccatg
     4921 ggcaactgct cttcgatctg gcgggcgatc gagctgatgt aggggctgca ataccagcgg
     4981 tcgtattcct gcggagtcat gctgacgggg tctttgcggg cgaaagcgtc gtccggcttg
     5041 gtgagcgccc ccacctgaat ccggctcacc tgacccggtc tgcccgtaaa atgctggaca
     5101 gtttccagtc tggtgaagat ctcgttttct tcatcgcctc cggtcacaag gactccgcgc
     5161 accacgaagg gctcgcccca caacttcagc gtcccgccgg gatcgacgtg cagccggcgc
     5221 gccaacaggg atcccaccag cgcttccctg gccgaagggt cgggagcagg atcgtcaatc
     5281 cactggcccc gcacgcgcca agtggggttc aggtcgcgca ctccggtcga aagcacctct
     5341 cgggtggccg tggtatagct ccggcggaac caggtgccgc gcacagccac cttgccgccg
     5401 cccatttcaa cggttgccga cagaaacggc gcgaatgccg tgatattgtt gcgccagaag
     5461 gtggatttga ggcgtgggag cgcttcttcg ggaataaaag tggcgtcggc caccggctgg
     5521 taatccaccc cgccgatttc gaccgggagc gctgtggact tgggagtcac gaccaggttc
     5581 gctccgaaac tgcgcaactc ccggctgacg cggtctccca catcgaccag aacggccagc
     5641 atagccgtag cgatcgcgct ggccagcgct actgccgcga tggtgctcaa tttgcgccgg
     5701 ggagagcgca tgagcgattg cccaaccagc cgtgcgaaca tgtttccctg cgtctcgaaa
     5761 gcgcgctttc cgccgcgcgc cttacaccct aaccgcccgc cgatggcttg acgaagcgcc
     5821 gtgcgcccgg ctccagatcc gcggcttcaa tcaccaaatc gcttccctcc acatgcgacc
     5881 ttactgcaat cgggttgcat ccgccggaag ccccaatgga aggaataaaa atagcggaag
     5941 cgcagttccg gcacaacaca ttggaacccc gctggtaata gccctggttt ccgcatatca
     6001 tgcaggcgtc gagcgcggcc acgagcgact gatccggttt ctgaatcaca atcagccgca
     6061 tcgcgacgcc ctgagcgctg gtatacgaaa agcgatgcag caggccatcg cttacggagc
     6121 gcaccggaat ccgcactttt ccgtcgaccg ccgcaagggg tattgccggc gtgagctggc
     6181 tttccgcccg ggcgtagacg aattctgcgg tcacaaacac aatgaacaga aatgaggcgc
     6241 tgcaaaccgc catcatccac aaacgctccc gtcgagccga ccagacagcc ttccggcggg
     6301 ccgcgccccc ggctggggcc gccgcttcct cgcgccggcg ccaatcaaaa aggaccatcc
     6361 aggcagccag ggccaatacg gtcacgaaaa aaaagacctc gtttttgaca atcggcccga
     6421 tccacgccat ctcgcgggcg cttgacggaa ggattccgcc ctccgaaagc tcgtgcaatc
     6481 cggtaatcag cagttgcaac acaactacaa aaaggattac ggtcgtgatg cggaaaaacc
     6541 gccgcaaatc gatgcgcacc gtgccgcgaa tgaaggtgac tccgaaaacg acggccagag
     6601 ccaggccgag caccagcccg gcggcttccg agagcgagtc gctggtgagg cttaccgctg
     6661 aaagcaacaa aaccgtctcg actccttcgc ggaaaaccat caggaagaca aatagaaaaa
     6721 ccccggcggc ggcgcccgac gagcggctgg aaatctcgcc caggcgccgc tccatcgccg
     6781 ctttcaaccc cttggaatgc cgccacatcc acacgaccat ggtgaagacg aaggcggcgg
     6841 aggcaagaag tgtccagcct tcaaatgcct cttcgctcca ctccagcttg cggaacaaat
     6901 acgcgccggc aacgctcgcc aggatcgcgg cgcccagagc ccggtacacc acgcggtgca
     6961 aatcttcgcg cccgaccttc cttagatacg agagggcaat ggcgatgacg agcgcagcct
     7021 ctacgccctc ccgcagcgca atgatgaacg aggcaaacat accggcgagc ggcagccaat
     7081 ccgctagatg aaagtgagtt gcaagtagcc attgtacgcc aggagacctc gggtgggcaa
     7141 gcgggtttgg gaagagcgaa tttcagcctg cgagccggaa aatatacagg atgggcgccg
     7201 cgatcgccgc ccggtgcttt cgattttagg tcactgcgcg ggaccgaaag caccggcgcc
     7261 gcatggtggg tgtattctcg gtcgcgccgt tggagatggc aacttgctca ttgcccccag
     7321 cccgcgggcg acggtggggc gatctagggg aaagctcgag gcttgaactc cccgctcgct
     7381 tcgagtggat gcgtcaggcc gctgccgcta cagtgcgccc agaaacgcga ccaggtccgc
     7441 tttttcctgt tccgtgaagc caatgccaaa ccggcggtca tagaagttca ccacatcgag
     7501 gagtgtcgcg gcggagccgc tatggaagta gggcgcccga gctgagaggc ccctcaaaaa
     7561 tgggcccctt caccttcccg atgtccgccc acaggcctgt tatcagggcg cgcccagggt
     7621 cggttgtctg aagtatggat cccgtgttct tattgcaaag cgttatcacc ggtaggtagc
     7681 tgacatccag aggctggtca ggtctcctgc gccgatgttg aggggcgcag gaaacgagtg
     7741 gtttcctacg ttgggcgtat cgtggcaggc gccgcaactg cctcgcagca cgctgactcc
     7801 gaggatgtcg tccacgccgg ccactccgcg gatgttgatg ggcttcgagt tgaatagcgc
     7861 ctggccgcgc gcgatgctgg ccctcctagg gttgccgtgt actcgctgga gccacgaatc
     7921 aaagacagtg aaaatgaccg gagtaaacgg cgcgcctgtg gggttgaagt gaaacgaagg
     7981 cggaaaagaa tcattgattc caatgaaaaa aggctgcgcg gaaagcggga ccggcccgcc
     8041 ggcggcgccg tcgccttgca gatctcccgc gtcgaaatcg agggcctgag ctaccgagag
     8101 ctggatctcg aaattgacga tggcccggag ctgttcgggc tataagtctt cgcggcttcg
     8161 agggggcggc gcatccggcc gcgcgggcgg gattgtaccg cgccggcggc gcacacagat
     8221 ctctctgcga gcaggaatcg aacgagcgga atttactggc aaaggcgcta cagcggccct
     8281 gcggccgaga ggagcgcctg ccggcgcgtt ttcaggggcc gaccgggatc ttgatgatct
     8341 tcttctgaat ggcgtattgg accaaatgcg ctttgttgtg aatgtcgagc ttccgcatca
     8401 ggttaaattt gtgggcttcg accgtcttga cgctcagatt caggcctgag gcgatctctt
     8461 tcactgattc gccctcagcc aggaacttca gaatttcctt ctcccgcgtg gtgagggtgg
     8521 cgaagcgcgg ctggcggtga gccgatttga tgcgcgagcg gaagtcgtca accaactggg
     8581 agagcatgcg ggggctcagg tagctgcctc cccggtagac gtcgcgaatc gcggccagca
     8641 actgctgggc cgggctgtct ttcagcacgt agccgctggc gcccacctcc atgcactgca
     8701 ccaggtaatc ctcgtcgtca tacatagtaa ggaacagcac cttggtgtcg gggcggacct
     8761 tcttgatctg ccgcgtagcc tcaaagctgc tgagccccgg catgccgata tccatgagca
     8821 ccaaatccgg ccgcagctcg gaaacctttt cgaccgcctc cgttccattc gccgcctcgc
     8881 cgacgatgtc taaatcggcc tcggccgaaa gcaaggtttt gatgccctgc cggaacaagg
     8941 tatggtcatc ggtaagcagg atgcgaattt tggccatagt aaatcctcag cttcgaagcg
     9001 ccgtcagatg gggatctgga tatcgatctt ggtccccttc atctggcggg ttttgatttc
     9061 catctttcct cccagcagcg ccacgcgttc gcgcatgccg gaaatcccaa acgactcccg
     9121 tttatgggcg acttgttcga gatcaaagcc gatcccgtcg tcctccacgt gcacgtgcag
     9181 gcggccgttg gaccggttta cagccagtaa aacattttcc gccttcgaat gttttatcac
     9241 atttgtgaag cattcttgca ctagtcgata caacattatt tcggtgtcct ggggaagctg
     9301 gcccagccgg gacagcttta accgcacgtg aatagggaag ttacggccaa aattgttagt
     9361 gaactggcgc aaagccgcgg ccaggccaag ctgctcgaga acgttcgggc tgagggcgga
     9421 aatcagccgc cgcatgtccc ggatggtgcc gtcgatcacc ttcagcgcgt cttccacttt
     9481 agtcgccaga tgcggcacat ggcgggtcat ttcctgctgc aacatctcca agtacagccg
     9541 caccaccaac atggcctgcc caacctcgtc atgcagctcg cggctgatgc gccggcgctc
     9601 ttcttcctcc accttaagca tgtgctcgcc gagctgccgg atctgctctt cccgctgggc
     9661 cagggtttcc atcaactggg ccttttcgag ggccagcgcg cagcgctcgg cgacggcctc
     9721 gagcagcttc atctcgcgcg gaaggcaatt gtactcgcgg gtaaaatcca gatgcagcac
     9781 gccggtgacc ttgcccttga cgatcaaagg cacgccccag aggcttcgaa tcgcgcggcg
     9841 aacctcggag gagcgtatca gcgggtcggc ccgggcgtcg agcaccactt gcggcttgcc
     9901 ggtctccgca atgcgccccg cgaagccctc gcccacccgg acattgaagt gccgggccag
     9961 ggccggatcc agcccccggc acgccttcac catgagctca ttctgctgtg gcagcagcag
    10021 cagcacgacc ccgccctggg cgttgaaggt ccgcatgagg ctgtccagca cgcgatttaa
    10081 caggtcgtcc acgctcagcg attcgagctc gtcctggaaa agatcaaaaa aggcctgggc
    10141 ttccaagtcg cggacttgaa agtacgccgt attcagggtt agaaggatgc agaagtacag
    10201 gtggtcgagc gaccagagat agttcgagta gtccttcggg aaaaagcggc gcaggtcggg
    10261 cagcagcgcg tcttcgtatt cgcggagaga gctgatgacg tgcgtcggcg gcacgtccag
    10321 cttggccagc cggcggccat tgtactcgac gcgctcaaag aaatcggcga gattacccgc
    10381 ggccagcaaa tcggcggccg cccccggatt gatcgccgcc agcgcccgca gccggggatc
    10441 gttgccccga agccccgaac gcgccagccg ccggcgccag cgattctcaa tctgctggct
    10501 gcaaggcgca atcctctcgg cgagacgccg gatatagtcc tgcacctggt cgctaaggac
    10561 gaccgcatct tccgggagga ccggtgtgcg ttctaagcca cgcatccttt gggagtataa
    10621 cccacctgat acagaatcct gtggcttttc ccacacactg gtcttccgcc gcgcgcggtc
    10681 ccggccgccg ggaggccgcc cggagcgcct tcagaggcgg ccgccggctt agcgcgaatc
    10741 ccccgcgcat gttaccctta aaagttgaac ctacctctga ggtattggac tgtccgattc
    10801 taaactgcaa gtcatcccat taggcgggct gggcgaattt ggcatgaaca tcatggccct
    10861 gcgctgcgat gacgatatcc tcgtcattga cgccgggatg atgtttcccg gcagcgaact
    10921 gctaggggtc gatatcgtcg tccccgacct cacctatcta aaggagaatc ggcagcatat
    10981 ccgggcgttg attctgaccc acggacatga ggaccatatc ggcgcggtcc cgtttctgtt
    11041 gggagaactg gacacgccgg tctacggcac gcctttcact ttagcgctcg tggagcggcg
    11101 cctggacgaa tatgatttgc cccgcaagcc ccgcctgcat ccggtaaagc ccaagcagat
    11161 catcgaaatc ggccggtttt cgatcgagtt catccacgtt acgcacagca tggtggactc
    11221 gaccgcactg gccatcgcca ctccgctggg cgtggtgatt cacaccggag atttcaagat
    11281 cgaccccagc cccaccgaca acgagctctt cgatctgcac actctagccg attacggccg
    11341 gcgcggcgtg ctgctgctgc tctcggattc gaccaacgcg gagtcccccg gcttcactcc
    11401 ctccgagcgc gctattcggg gccgcatgga agacattttc atgcgcgctc aggggtcgct
    11461 gatcgtctcc tgtttcagtt cctcgacgca ccgagtccag cagatcatcg atttgtctta
    11521 tgagtacaat cgaaaaatcg cgctggtggg ccgcagcatg atttctacgg ccgaaatcgc
    11581 ccacgatctc ggctttctca agattcctga cggcctgctg atccgccccg ccgagatcga
    11641 tcggctgccg cgctcgcagc gcacggtgct catcgccggc agccagggcg agccgatgtc
    11701 cgcgttgtcg cgggtgtccg tcgataaaca caagcacgtc tcggtcgcac ccggcgacac
    11761 ggtggtgctc agttcgcggg tgattccggg caatgaaaag gccatcttcc gaatgattga
    11821 ccacctttcc cggcgcgggg ccgaagtgat ttacggatcg accacgccgc cggtgcatgt
    11881 atccgggcac gccagccagg aggagctcaa tttgatgctc aacctggtgc gcccgcggtt
    11941 ttttgtcccg attcacggcg agtaccgcca actggcgcgc catgccgctc tggccgagca
    12001 tctgcgttac gccggactcg aggagagctt tgtgctcgag agcggaaacg tactcgagat
    12061 tgattcccat ggcgcccgca aagccggctc ggtcgccgtc ggccgcgtgt gcattgattc
    12121 ggacgcagtg gacgaagtgg tggaggacgt aattattcac gaccgccggc acctctccga
    12181 agagggcatc gttctgaccg tcatcgccat caatcgccat accggcgaga tcgagagcca
    12241 gccggaaatc gttacccggg gctttgtggc gcccgaggac ggcgccgaac tggtctcccg
    12301 ggcgcgcgag atcgtgatca gcacgctcga aaactcaacg ggcgaagaaa agaccgacta
    12361 cggcgtcatg aaggccaaaa tcagcgccga cctgaagcgc tttatcgcgc gagaaacagc
    12421 caagcgcccg ctgatcctgc ccgtcatttt ggaagtctag aatccgcaaa ctggatatgc
    12481 cgcctccctc tcgtcccgtt cgccgatgcc ggcggggact gcccgtcccg cgagcttctt
    12541 cgattcgagt tggctgtttt tcgcgctccg tgtagttgaa ggtcagaagg aatacagccg
    12601 cggcatgggc ttcacgccgg caggctctgc gccgcaagac ccaacccgac cggaaaccgg
    12661 agagggcagg catcgcggcg ctttcgtagg gccatagcgt ttttggcgct tcgcgatctg
    12721 tggacctggt tttcccgcat tgcattggcg gctgccggtt gagaaaattc aagaggtgag
    12781 ctatgcaagc gactcagatg gaatccttaa actacgttga tggagagtgg cgatccagcc
    12841 gctcctccga tctctatgaa gttcagaatc cggctacttt agaaactctg gcgcgagtgc
    12901 cgctggcagg gccggaagaa gtggacgccg ccgtagccgc cgcggcgagc gcgtttccca
    12961 cctggcgccg gatgccgccc caggatcgca ttcagtactt attccggttc cgggatcttc
    13021 tgatccagca cgccgatgat attgcgcgca tgatcacccg ggaaaacggc aagactctgg
    13081 ccgagtcccg gggagagttg cagcgcggca ttgaaaatgt cgaggtagcc tgcggaatcc
    13141 cgacgctgat gcagggctac aacctggagg atgtggccag cggcatcgac gaaatcatga
    13201 ttcgccagcc gctgggcgtg accgttgcca tcaccccgtt taatttcccg gcgatgatcc
    13261 ctctgtggtt tctgccatat gccatcgcgt gcggcaatac tttcgtgctg aagccctcgg
    13321 aacgggtgcc tcttaccgcg cgcctgttgt tcgagttgct gcatatcacg ggcctgccca
    13381 agggagtagt aaacctcgtg gtcggcgcga aaccggcggt cgacgctctc ttgcgacacc
    13441 cccaagtcaa agccgttagc tttgtaggct ctacaccggt ggccaaatac atttacgcgg
    13501 aggccgcagc ccacggcaag cgagtccaat gccagggcgg ggcgaaaaat catgttatcg
    13561 tcatgccgga cgccgacctg gccatgaccg ccaaaatcgt gagcgacagc gcttttggct
    13621 gcgccgggca gcgctgcctg gctgtttcca ccgccgttac tgtcggcgac gcctcgaaga
    13681 gttttccgag cgcgctgctc gagctggctt cctccatccg cgtcggcaac ggtctgtacg
    13741 aaggggtgca aatgggaccg gtgatctcgc cggcgagcaa ggcgcgcatt ctgaacctga
    13801 tcgagaaagg aatccacgat ggagggaagc tcttgctcga cggccgcaac cccaacctgg
    13861 ataacggcga acgcggcaac ttcctgcggc ccactgtcat cgccgggctc ggcggccaaa
    13921 gcgcgcttac cgataccgag attttcggtc ctgtgttgac gctgcgcgaa gtctccagcc
    13981 tggatgacgc catcgaaatc ctctcgcgca gcccttacgg aaacgcggcg tccattttca
    14041 ccagcagcgg ccacacggcc cggaaatttc gctatgaggc gcccaccgga aacgtcggaa
    14101 tcaatatcgg ggtcgcggcg cccatggcct acttcccgtt cagcggctgg aaagacagct
    14161 tcctcggcgt cttgcacggg caggggcgcg acgcggtgga gttttacact gataagaagg
    14221 tcgtgattga gcgctggccg aaagagtggt cgcgaaagtt ttgatccagc cgcgcgaatg
    14281 gctcggggcc agcaaaggct gcgcggtcag cgaaatgggt ctgacttcac gatgccttcg
    14341 ccttcccgta cctgaatcga ccgattggcg gagatgttac tgagcttctc caggattccg
    14401 ctcggccaac ggatttcgat gtcatccact tttgccgcgc cgcccaggcc aaaatgcagc
    14461 cggaagtcgc tctgggacgc gtagctgccc ccgctgcgca cttcatcagt ttggcgcaac
    14521 tcgccggcgg tcaccgttac tctggctccg acggcgctgc ggttggattt cgttcccgtt
    14581 aagccgatca ggatggcatt gcccttgggc ccgaagttct tgaggaccgt gggacgttcg
    14641 ttcatgttaa cgacaacaat ctccagggcg ccgtccccgt ccagatcgcc ggaggccacg
    14701 ccgcgggaaa ccgcgggagc cgatagcgcg gcgccggccg agcgcgtgat atcccggaac
    14761 gcgccgttgc gaaggttcca atacagggcc ttcggctgcc ggaagtcctc accgacgcgg
    14821 gcgccggcca gttccgggta aatgtgccca ttggccatga aaatatcttt ccagccgtcc
    14881 tgatcgacgt cgatgaaatt cactccccag cccacgacct ttgtgttcac accgaggccg
    14941 ctaagatacg tttggtcctc gaaataggca tcgccttcgt tgtggtagag cgtgggagtt
    15001 tcgtccatga aattcgtttt caccagatcg atgcggccat cgttgtcata gtcgccggcg
    15061 gccacgccca tactgccttg ctcctgccca tccggcccgt aagcggcgcc gcgcccgact
    15121 gcaacatcgg tgaatgaccc gttacggttg ttgcgataaa gaatgctggg gctggaatcg
    15181 caggccacat agatatcggg ccagccgtca tcatcgaagt ccgccgtgac cacgcccagg
    15241 ttgtagtgca actcggaaaa ggcaatgccg gctttctggg tgacgtccgt gaatgcccgg
    15301 ccgcggtcgt tgtggtaaag aaaattgcgg ccagccggat agccgcgggg accgcagaaa
    15361 acacgcaacc ccttccacgt gcagaaaggg cttgcgcccg gcaaagcagc tttttccagg
    15421 tcgaagtcca aatagctggc gacgaatacg tcaagccagc catcgcggtc gtaatcgagg
    15481 aaggcgcatc ccgtgttcca gcgctgtcct gcggcgggca gccccaactt agccgtcacg
    15541 tcctcaaagc gcccgccgcc cagattgcga tagaggctgt tatgcccata ataggtgaca
    15601 aacagatcag tcaggccgtc gttatcgaag tccccggcgc atacgccctg accccatccg
    15661 ctgcgctcga gaccggaatt tcgggttacg tcccgaaatt tcccatttcc gtcgttgtga
    15721 tagagccggc tggttggctt ttctccggcg ggcaggccat cgagacgggt tccgttgacc
    15781 aggaaaatgt ccggacggcc atcattgtca aaatcgaaga cggcagcccc gttgccggtc
    15841 atttccaaga tgtacttctt cgatttcaca ccaccgtaaa cattgggcgc cgtgaggccg
    15901 gcttccgccg cgacgtctcg aaattccacc gtgggactgg gcagatccag gcgggctctc
    15961 ggagagggcc gcgcggtcat cctgcctcca cccatgcctt gactgagaag caaggggaca
    16021 aaggccagaa cgcccccgaa aagccagatc ccccgtacat aactggatag agccatcgct
    16081 gcgcagactc ggaacatacc gcttttcgaa tcacaattca aaccttcgcc acccgcgccc
    16141 agcgatcgac gggaaccccg cgtaaccagc cgattccgcc taaattctag taatataatc
    16201 taacactata gtattttagg cgaaatttcc cttcccgcac cgccttcctg cgccttcccg
    16261 ccaagggtga tggccgcgcc accagcgtat tttttcttgt tttttctttt tcggacgggg
    16321 ataatggcgc aaacgtggtt tccaggaaaa gggggaggtg ttttatgtta tcgctccggc
    16381 agaagacggc cagatgcctt tccgcgcttc tggtagtttt gggtggggcc gccctggctt
    16441 ttggtcagga gaccacaggc ggcatcgtgg gaactgtgaa ggacagttcc ggttcggtga
    16501 taacgggcgc ggtcgtaacc tctgtgaaca tcgaaaccga tgtgcaatct cagaccacaa
    16561 ccgacgaacg agggaactac gaattcccgc tgctgcgcgc cgggcggtac cgcctttcag
    16621 tggaagctcc aggttttcaa cgcctggtgc ggccggaggt gattgtcaac acgacggagc
    16681 gggtgcggct ggatttgacg ctgcaagtgg gggcggtgag cgagacggtg acggtaactg
    16741 ctgaaacccc gctgttacaa agcgaaaaag cgactgtcgg ccaggtggtc gagcagcgca
    16801 cgatcgaatc aattccgctc gcgacgcgaa acttcaccca aatcctggga acttcggcgg
    16861 gcgtggtcgg ctccatcttc aacgccgaca cccccgggac cggcagcgac agcgtgagcg
    16921 tgaacgggtc ccgccgcggc agcaacaacc tgctggtgga cggcgtgcag accaccaaca
    16981 ctttgaacaa tgcgccggac ggcgacggca cgccgagcat cgaatttcta ggcgaattca
    17041 aggtaatgac gagcttgtac agcgccgagt acgggcgtaa ttccggctcg atcatcaacg
    17101 taaccacccg cagcgggacg aatcagtttc acggagccgc gtacgagttc ttgcggaaca
    17161 cggacttgaa ctcgcggcct tttttcaacc cggcccgggg cgaaaacatc cagaaccagt
    17221 tcggcgcgaa catcggcggc ccgatcaagc acgacaagat cttcttctgg ggcgggtggg
    17281 agagcatgcg gcagcacaat gccaactcgg cgctgggatc ccaggggagc agcaacggcg
    17341 gggtcgtgat caccgtggtc ccgacgcttg ctcagaggca gggagatttt tcggcgtttc
    17401 cgaaggcgat caaggatccg acgaccgggc aagcattctt aaacaacatt attccgcaat
    17461 cgcgtttgaa tttgatttcc ctgaatattc agaacgcgtt tgtgccgctg ccgaacttca
    17521 gttcgggcaa caacaacttc ttcgccgcgg ctccgatcac taccaatctt gaccagggcg
    17581 ccgggcgcgt ggattaccgc ctcagctcaa gtgatacgct gtacgggcgc tggttcgaaa
    17641 gtatggagtt tgatctgacg cctttcggcc agggcatgcc cggcttcaca cagggatcga
    17701 cgcgggcaaa gcatacgggg ggattcagcg agacgcatat cttttcgcca gcctgggtgc
    17761 tggaatcgcg tgtcggaatc gacatgacag accagtatct ctcgttcggc aacaacactg
    17821 atcccaccac cctggggttg cagccgatca agggagtgac tttgatcgct ggcctgccca
    17881 agatcaacat cagcaactac gtgaacggcg gctttgggaa ctccagccgc tggcatgaca
    17941 acatcaaaac gttcaccact tcctccacga tgacctgggt gcgctcccag cacacgatga
    18001 agttcggggc ggagagcgtt acctcgctgc tgcacccgtt caatgatcag gccagccggg
    18061 gcacgtggag ctttacgggt ctcgctaccg gcaacgaata cgccgatttt ctgctcagcc
    18121 agccatctac gaaacagttc gcggcaggcc cgggagagct gctgatgcgc gagcaggtct
    18181 ggagcggctt cttcgacgac agttggaaag cgacccagaa cctgactatc gaactcggct
    18241 tgcgctacga agcccatttc aatccggccg cctacaatct gcacatggtc agtttctggc
    18301 cggaccggta ccagggagtg ggctcggtgg acgccgcggg catcgtccag ggcggcgtca
    18361 ctcccggtgt gccgaatgac gtggtgttcg ggcaatggca cgatttcggc ccccggatcg
    18421 gcattgctta ccggatcggg gataaatggg tcattcggac cggcgccggg atctactacg
    18481 atcaacgcac gggccagatc gcccaaggca cgttctccaa tccgccgaca ttcaagaacg
    18541 tcactatcaa ctgcgcggtc gcaggccagg tttgcagcct gacccaaccc gacaactgga
    18601 ccttcgtcga ccccggttac gatccgaaag ccattccgtt cccgaccaaa accaccgatc
    18661 agacccttac gctttccgcg attgatcccc gagcaaagac ggatcgcgtg atccaggaca
    18721 acttcaccat ccagcgccag ttgccgaaaa acaccctcct tgaggccggc tacgtgggga
    18781 ccaaaggcac ctacttggcc acttcgtata atgtgaatcc actcatgccc cagccggatg
    18841 gcacgctggt gcggcgtttc ccgggttttg gcagcatcaa ttaccgcaac gatatgggaa
    18901 gttccactta ccattcgttg cagatgaccc tgaagcgccg cgtagccaca tcgacgttcc
    18961 aactctccta caccgcgtcg aaagtgctca caaacgggaa tgagtcggcc cgctactcga
    19021 cggccctatt cgcagcgccc tggaacgatt ggagccgggc aaagggtccg gccaactttg
    19081 accgcccgca gcggctcgcc ttcacctggg tgcaggatct gcccagcaag gctacgagcc
    19141 ggctcgggag atttttcctg aacaattggt ccgtcaacgg attcttcatc gcgcaatccg
    19201 gattcccgct gacagtgatc aataccacca gcggccaggg cctcggagga accacatcca
    19261 acgacaatgg cagctttttc gcgaacgtcg tggcgggtgc tcccttaatt caccccaccg
    19321 gctcgactaa ggacagcctc cagagttaca tcaacaaggc tgcctggatt ccggcgccgg
    19381 cgggcaccta cgggaattcc ggacgcggca tgttccgcgg ccccggacag tggaacgtgg
    19441 atgcctccgc ttttaaggat ttcagcatca ccgagcgctg gaaaatggag ttccgcactg
    19501 agtgcttcaa tgtgttgaac catgccaact tcgcgctggg cactgacacc agcacgtcgt
    19561 tgaacatgaa ttcttccacg ttcggacaga ttagcgggac ctcggtaaac gcccgcctga
    19621 tccagtttgc tctgcggctg agtttttgag acaaaccgac agcccaggcg gcggccgcca
    19681 gccgtggata gccctgcacg gcgacttctg gtgcacctgt ttcaaagccg ggccctcgag
    19741 aaccgcatgt tcctgctgcg cgttaattat tcggcgccgc acaataacgg cggcagcgcc
    19801 atctttgatt tcgaaggatc gatccaggac gaactcgacc agcgggaagg cgtgctggtg
    19861 ggcaacctga acctgactgc tctgcgcaaa atccgcaacg cctggaacaa ggtatacggc
    19921 cctgaaaacc gttatccgga agcctaccgg cggctgcggc aggaatcccg ttgaacggcg
    19981 ccatcaggcc tggcggcgca ccctgaaggc ccgcgcgcct gacgccaacg gctcaaataa
    20041 ccgtcagggc tggtaacgca cgaaggatgc gcctgcaagg aggagcttca cagcagatcg
    20101 agcgccgctt gcagctccga tctggcgtgc aggtcgccgg cgcgatccgc ggcttcgatt
    20161 ccttgggaat aaatccggcg agcttcttcc atgcg
//
LOCUS       DASREK010000017        11724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_670792, whole genome shotgun sequence.
ACCESSION   DASREK010000017 DASREK010000000
VERSION     DASREK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670792"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFA54_01395"
     CDS             <1..1333
                     /locus_tag="VFA54_01395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS49487.1"
                     /translation="VSLEELGGHQLQDYLLFRPEAAAREISARLRAGNRCFVARSQGR
                     IVHARWAIITGPARCEVLGVELPIAKDCAYVCESLTLPEMRGLGIAAAADAFMLRRLR
                     ESGLRAAALVVDPDNRAGIRTAQKTGYRAAGSVAVVRLGPWRRLVRWSGVREPEPAYW
                     NNIGIKLLPGNTYLDPFLARLKRRIHLRLIRHWTQGMPPGARILKTDLFEEANGRDAL
                     IPGLACGGRRVFGVDISPAITARSVGSVPDSARLAAADVRALPFADGVFDLVISPSTL
                     DHFRDPADLGRSLQEIRRALHPGGRLIITLDNRQNVLDWLLRAASRLGITPYYLGRSY
                     TIHELRQELAAAGFSVLDATGIVQTPRLMAMTVAAAARWVNWRSFTRFVHRNILRAQR
                     LEQTAWKYWTACFVCACAAPGGDRTLGAREAWEGCSMTVESGGAPLSRRSR"
     gene            1350..2699
                     /locus_tag="VFA54_01400"
     CDS             1350..2699
                     /locus_tag="VFA54_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49488.1"
                     /translation="MPGWLTRIRWSAFTAWHLRHESSLPFWPLERVLAVQERRIREIA
                     AFAAREVPFYREAFRSAGLRPEECRSAADLARLPLLRKEEVSRHPERFRPERPNRASL
                     RLHSSGTSGKARTIDYDDAALFLALAHGHRQRLVLARVLGRTTGYREAVIARPEGLPA
                     QIRRFYETHSWFPSRVELERIVISPAESYTEICRRLEEFQPHAVFGYGSHLGAFYRWV
                     AEKNSGGHLPRVIFYGADRMPDADRALIEEKLGILVFSFYQSAESLRIAFQCERRQGL
                     HLSLDQIAVRVIDSSGRDAAPGGRGEIVISNLTNRATVLLNYCLGDIVTVGSGPCPCG
                     RSLPVIDRIEGRADDLVRLPDGSSIHALSTLEGIQAVPGVIQVQIVQEELRRFLLRAV
                     CSQGADWPAIEKRFASVLSSTLGDGLTLQIERVPDIAPDSSGKVRAVISRITGLNSP"
     gene            complement(2796..4199)
                     /locus_tag="VFA54_01405"
     CDS             complement(2796..4199)
                     /locus_tag="VFA54_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZS49489.1"
                     /translation="MNAQERFDRFIQRYPQPRRSFLDRPHFTRRQFFQILGCGVTGAC
                     LLPSLRAVPVVRQTPVQTQNKAKSVIFILLQGAPSHIDTFDFKTVPGVTPDNFNPETI
                     SGITWPTGLLPNLANNLPDLAIVRSMRAWALVHSLAQRWTQIGRNPAAALGDIAPNIG
                     SVVALEKDSERTPNQVFPTFIALNSNSAAGPGYFAATYAPFEIAPSSKGLANTTNPDG
                     QALSNERWGQLHALDDPLRVNSPLGRPLEDYDNFYTAARSLMYNPTVQQAFAFSAADS
                     QRYGSSSFGNACLVAKQILEANQGTRFIEITLGSWDMHQNIYGQTNPNGNNLYTLGKT
                     FDLGASALLSDLKSSGLLDETLVVMVGEFGRTVGPLSAAGGRDHYPQQSIVFAGAGVK
                     GGRAIGSTTDDGSMTADPGWSRGRDVKPEDVEATIYSAMGINWTTVRYDDPFGRGFEY
                     VPFSDQDVYGPIDELWE"
     gene            complement(4347..6323)
                     /locus_tag="VFA54_01410"
     CDS             complement(4347..6323)
                     /locus_tag="VFA54_01410"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZS49490.1"
                     /translation="MSIRAMKRGGFWIALLSISVPLGIFRLRVLRAQDESEPQLPVAH
                     PECVYFGPQHERYARQGLSMPGTSSSQLSLSALTQQVVRQLPPLSSATRSGALQPLAQ
                     MGPIDRNLFAAMQAAGVSPAPATTDFEFIRRVTLDLTGRIPAPDRVIRFVNDPSPTKR
                     EALVEELLAKPEWVDKWTMYFGDLFQNNSRNSQVARFAQGRNAFYNYIHDSLAANKPY
                     DQMARELISAQGTDSFQQGELNWMVGGVVTGGPVQDIMDQQAANIADTFLGIAHMNCL
                     LCHNGRGHLDQLSLWGSQGTRYQAWQFASFVSRTVVARVPMSGQTNLYSWSVVDNPAR
                     ANYPLNTTTGNRPARQPSGTPSGTEKNVAPVYWFTGAKPAPGQNYRAALAQFITSDFQ
                     FARAAVNYIWAQFFGRGIVDPPDQFDLARLDPNNPPPAPWTLQPSNPALLQELAQDFV
                     NSKFDLKALMREIVNSRAYQLSARYDGPWDPAWEPLFARKLVRRLWAEEIHDAVAQSS
                     NVIPSYTITQNGATFGPVNWAMQFPETAGMPAGNAAVVQFLDAFLRGNRDDQQRRGDG
                     SISQALDLMNDNFIMSRIRATGSGASASLLARSLSLPDDQLVNNLFLAVLSRYPTPAE
                     MSTALANLQSGDRTQEAQNLLWSLYNKVDFVFNY"
     gene            complement(6713..6889)
                     /locus_tag="VFA54_01415"
                     /pseudo
     CDS             complement(6713..6889)
                     /locus_tag="VFA54_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
     gene            complement(6905..7033)
                     /locus_tag="VFA54_01420"
     CDS             complement(6905..7033)
                     /locus_tag="VFA54_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49491.1"
                     /translation="MERRRMLIALGVAGLSFLAPAFASPAAPPKEATVTLVIDGMT"
     gene            complement(7170..7913)
                     /locus_tag="VFA54_01425"
     CDS             complement(7170..7913)
                     /locus_tag="VFA54_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS49492.1"
                     /translation="MFLSLEGQTAIVTGAAEGIGRAIAARLAKAGATVAVADYNENGA
                     LAAARDLGGAAFGVGVDVSDNASVGRMVNAVLERTRRVDVLVNNAGIGGRAAPVWEQS
                     DEDWDRVLRVNLTGVFYCCRAVVPHMRAQRYGRIVNIASIAGKEGNPNMCAYSASKAG
                     VIAFTKSLAKEVATEGICVNSISPAVIRTRILDQLTPAQVDYMTERIPMRRTGTVEEV
                     AAVAHFLASRDASFVTGQCYDVSGGRATY"
     gene            complement(7923..8369)
                     /gene="rplI"
                     /locus_tag="VFA54_01430"
     CDS             complement(7923..8369)
                     /gene="rplI"
                     /locus_tag="VFA54_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZS49493.1"
                     /translation="MEVILKAEVPKLGGRGDVVKVADGFARNYLLPRKLAIEATQGSK
                     KVIEDMKAAALRREAKLKSDAEKLAEMMKELTVTVAEKAGENDQLFGSVTSKTIAEEL
                     AKQGYNIDRHKIEIAKPIRMLGEHEAVVNLHKGVSTTIKVVVTKAE"
     gene            complement(8476..8781)
                     /gene="rpsR"
                     /locus_tag="VFA54_01435"
     CDS             complement(8476..8781)
                     /gene="rpsR"
                     /locus_tag="VFA54_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZS49494.1"
                     /translation="MAEARRPAGGPRGAAGEKSGTPGAPSGKKQYFRRKKVCRFCVEK
                     IDNINYKEVKLISSFVAERGRIIPRRISGVCTPHQRQLSDAIKKARNIALLPFAAEL"
     gene            complement(8818..9243)
                     /gene="rpsF"
                     /locus_tag="VFA54_01440"
     CDS             complement(8818..9243)
                     /gene="rpsF"
                     /locus_tag="VFA54_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZS49495.1"
                     /translation="MRIYEILFIIRPDVPEEEIDASIEPLKNVITAAGGVVDKVDKWG
                     KRKLAYRVKKHREGYYVLLQFSTEKGSETVKELERRLRVSDTVIKFLTVRIDEELKRL
                     EKLKSRREKRALRKPPPAAVEPPSPGATPQIPAEAAVKE"
     gene            complement(9240..9827)
                     /gene="pth"
                     /locus_tag="VFA54_01445"
     CDS             complement(9240..9827)
                     /gene="pth"
                     /locus_tag="VFA54_01445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZS49496.1"
                     /translation="MFLIVGLGNPGPEYANTPHNLGFMTIDRLAQIGGLSVHRPECQS
                     LVGRGVIEQRDVLLAKPQSYMNLSGGPVKALLAKYKLTPADLILVYDELDLPWMSLRI
                     NLRGSSAGHRGVESVIGALGTKDFTRVRLGIHPGRPVDAADYDLTPFRRSQQKEVEEL
                     VSRGADAVRSILAEGAAKAMTRFNRRAGGQITEGK"
     gene            complement(9922..10635)
                     /locus_tag="VFA54_01450"
     CDS             complement(9922..10635)
                     /locus_tag="VFA54_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZS49497.1"
                     /translation="MIKDITVEAAPRAQRGKNEARRLRRQGQIPAVLYGGGKDSVPVS
                     VNAKQINHILQSATGHNTIFQVAIAGGSREAAMLADWQVDPLRGDLLHTDLLRIDLTK
                     SLKAMVPIVTQGDAKGVKTQGGLLEIVTREVEVECLPQNIPEHITINVTDLGLGEAIR
                     VKDVPADERYRLTSDPEKILVHVIMLKKEEEKPAEAVVEEAAAAAPAEPEVIKKGKQV
                     EEGEAAEGEKKEGKEKGKK"
     gene            complement(10755..11699)
                     /locus_tag="VFA54_01455"
     CDS             complement(10755..11699)
                     /locus_tag="VFA54_01455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZS49498.1"
                     /translation="MQHDRLKVFTGNANPKLTDEICKHLGVPRAEASVERFSDGEIYL
                     QILENVRGADVFVVQPTCTPVDQNLLELLLMMDALKRASAKRTTAVLPYYGYARQDRK
                     DKPRVPISAKLVASVLVTAGADRILTLDLHAAQIQGFFDIPVDHLFATPVMIEYFQHL
                     DLPKLTVVSPDAGGVERARAFAKRLKAPLAIIDKRRDQPNVAEIMHIIGRVRDRTCLI
                     VDDIVDTAGTLVNAAEALLGQGALAVYACCTHPVLSGSAVERITRSNLKELVVSDSIP
                     LSEAARQCDRIRQLSVAPLLAGAIQSIHEETSISRMFV"
BASE COUNT         2098 a         3623 c         3573 g         2430 t
ORIGIN      
        1 ggtctcgctc gaggaattgg gcggccacca gcttcaggat tatctgttgt tccgtcccga
       61 ggcggccgcg cgcgagatca gcgcgcgcct gcgggcgggg aaccggtgct ttgttgcgcg
      121 atcgcagggc cgcatcgttc atgcgcgatg ggcgattatc accgggccgg cgcgctgcga
      181 agtgctcggc gtggaattgc cgatcgccaa agactgcgcg tatgtttgcg agtcgctaac
      241 ccttcccgaa atgcgcgggc tcggcattgc agcggcggcc gacgccttta tgctgcggcg
      301 cctgcgcgaa tcgggacttc gggcggccgc gctggttgtc gatcctgaca accgggcggg
      361 catccggacc gcgcaaaaga ccggatatcg cgctgctggc tcggtggcag tggtcaggct
      421 gggcccgtgg cgccggctgg tccgctggag cggggttcgg gaacctgagc cggcctactg
      481 gaacaacatc gggatcaaac tgctccctgg caatacgtac ctggatccat tcctggcccg
      541 gctcaagcgc agaattcatt tgcggctgat tcggcattgg acgcagggaa tgccgccggg
      601 agcacgcatt ctgaaaaccg atctgtttga agaggccaac gggcgggatg cgctcattcc
      661 cggcttggcc tgtggcgggc gccgcgtttt cggggtggat atttccccgg ccatcacggc
      721 gcgctcggtc ggttcggtcc cggattccgc ccggctggcg gcagccgatg ttcgcgcttt
      781 gccgtttgcg gatggcgttt ttgatctggt gatttctccc tccacgctgg accatttccg
      841 cgacccggct gacctcggcc gaagcctcca ggaaatccgc cgcgcgcttc atcccggcgg
      901 gcgtctgatc atcactctcg acaaccgtca aaatgttctg gattggctgt tgcgcgccgc
      961 ctcgcggctc ggtatcacgc cttactacct cgggaggtcc tacaccatcc atgagcttcg
     1021 gcaggagttg gcggccgccg gtttttcggt tctcgacgcc acggggatcg tgcagacgcc
     1081 ccggttgatg gcgatgaccg ttgccgcagc ggcgcgatgg gtgaactggc gttcctttac
     1141 gcggtttgtt caccggaaca ttttgcgggc ccagcggctc gagcaaacag cctggaaata
     1201 ctggaccgcg tgttttgtat gcgcgtgcgc cgctcccggc ggagatcgaa ctctgggtgc
     1261 ccgggaggct tgggagggtt gttcgatgac tgtggagagc ggcggcgcgc ccctctcgcg
     1321 ccgatcccgg tgatttcgcc cctacagtga tgccgggctg gctgacacgg attcgctgga
     1381 gtgcgtttac cgcctggcac ttgcggcacg agtcctcgct ccctttctgg ccgctggagc
     1441 gggtgctggc ggtgcaggag cggcggattc gcgaaatcgc ggccttcgcc gcgcgggaag
     1501 ttcccttcta tcgcgaggcg tttcgctccg ccggcttgcg ccctgaagag tgccgcagcg
     1561 ccgccgacct ggcgcgtctt cctctccttc gcaaggaaga ggtaagccgc catcctgagc
     1621 gcttccggcc tgagcgcccg aatcgcgcca gcttgcggct ccactcgagc ggcacctcag
     1681 gcaaggcccg gaccatcgac tatgacgacg cggcgctttt tctcgcgctg gcgcacggcc
     1741 accgccagcg gctcgtgctg gcgcgggtgc tgggcagaac cactggctac cgggaagccg
     1801 tcatcgcccg ccccgagggt ttgccggcgc agatccggcg cttttatgag acgcattcct
     1861 ggttcccatc ccgcgtggag ctggagcgca tcgttatttc tccggcggaa agctacaccg
     1921 aaatctgccg acgtctggaa gaatttcagc cgcatgcggt tttcggctac ggctcccacc
     1981 tgggcgcctt ttaccggtgg gtggctgaga aaaatagcgg cggtcatttg cctcgggtca
     2041 ttttttacgg cgccgaccgc atgcccgacg cggaccgggc tttaatcgaa gaaaagctcg
     2101 gcattcttgt tttttccttt tatcaatccg cagaatcgct gcgcatcgcg ttccaatgcg
     2161 aacgccggca gggtcttcat ctgagcctgg accagatcgc ggtgcgcgta atcgacagct
     2221 ccgggcgtga cgcggctccc ggcggccgag gtgagatcgt gatatcgaat ctgacgaacc
     2281 gcgccacagt cctgctgaat tattgcctcg gcgacatcgt cactgtcggc agcgggccgt
     2341 gtccctgcgg gcgaagcttg ccggtcatcg accggattga agggcgcgcc gacgacctcg
     2401 tacgcctgcc tgacggctct tccattcacg cgctttctac cctggaaggc attcaagccg
     2461 ttcccggcgt aattcaggtt cagatcgtgc aagaagagct gcgaaggttc ttgctgcggg
     2521 ccgtttgctc gcagggggct gattggcccg ccatagaaaa acgctttgcc agcgtgctct
     2581 cttcgacgct tggagacggc ttgacgcttc agatcgagcg cgtgccggac attgcgcctg
     2641 attcgagcgg gaaggttcgc gctgtgatct cgcggataac cgggttgaat tccccgtagc
     2701 acccgccggg agccgatgtc gggcctgaaa tctttaaacc ttgcgcgctg cggaacaacg
     2761 gcagcgcgca gagatgtccg gggtcacgcc agcttctact cccacagctc gtcgatcggc
     2821 ccgtacacat cctgatcgga gaagggcaca tattcgaagc cccggccgaa agggtcgtcg
     2881 tagcgcacgg tagtccaatt aatacccatg gctgaataga tggtggcttc gacgtcttcg
     2941 ggcttgacgt cgcggcccct ggaccatccc ggatccgccg tcatggagcc gtcgtcggta
     3001 gtagaaccaa tggcgcggcc tcctttgacc cccgccccgg cgaagacgat tgattgctgc
     3061 gggtaatgat cgcggccgcc ggcggcggaa agcggcccga cggtgcgtcc gaactcgcct
     3121 accataacca ccagggtttc atccagaagg ccgctcgatt tcaagtccga taatagggcc
     3181 gaggcgccga ggtcgaaggt cttgccaagc gtgtagaggt tgttgccgtt gggattcgtc
     3241 tgcccgtaga tattctgatg catgtcccaa ctgccgagag tgatctcaat gaaccgcgtg
     3301 ccttgattcg cctcgaggat ctgcttggcg accaggcagg cgttgccgaa gctggagctg
     3361 ccatagcgct ggctatcggc tgcggaaaag gcgaacgcct gctggacggt ggggttgtac
     3421 atcaagctgc gagctgccgt ataaaagttg tcgtaatcct cgaggggtct tccgaggggc
     3481 gagttgacgc gcagcggatc atccagcgcg tgaagttgtc cccaccgctc gttcgagagc
     3541 gcctgaccgt cggggtttgt agtattagcg agacctttcg acgacggcgc gatctcaaac
     3601 ggcgcgtagg tggcggcgaa gtagccgggg ccggctgccg agtttgagtt aagggcgatg
     3661 aaagtcggga atacctggtt gggagtccgc tccgaatctt tttcgagtgc aacgacgctg
     3721 ccgatattgg gcgcgatgtc gcccagcgct gccgccgggt tgcggccgat ttgagtccac
     3781 cgctgtgcaa ggctgtgcac cagagcccag gcgcgcatcg accgcacaat cgccaagtcg
     3841 gggagattgt tggccagatt cggcagcagg ccggtcggcc aagtgatgcc cgaaatagtc
     3901 tccggattga agttgtcggg cgtgacccca gggacagtct tgaaatcaaa cgtatcgatg
     3961 tggctgggag cgccttgcag caggatgaag attacgctct tagccttgtt ctgcgtctgc
     4021 acgggcgtct gccggacgac aggaaccgcg cgcagactgg gcagcaggca cgccccggtc
     4081 acgccgcagc cgaggatctg gaagaactgc cggcgggtga aatgcggccg gtcgagaaag
     4141 gatcgccggg gctggggata gcgctggatg aaccggtcga atcgttcctg tgcgttcatg
     4201 acaagacctc tctgggccgc cccgagccgc gcgaccgggc gccgcgggga gcgcgcaaaa
     4261 agttggacaa gtacaaggcc ggaggcccat ccggctcctc gcagccgctt cgccctctga
     4321 acagtggctt catctgatgc tccagctcag tagttaaaaa caaaatccac cttgttgtag
     4381 agggaccaga gcaggttctg cgcttcctgc gtccggtcgc ccgattgcaa attcgccagc
     4441 gccgtgctca tctccgcggg cgtcggatac cgcgagagaa cggcgaggaa caaattgttc
     4501 accagctgat cgtcgggaag gctgagactt ctggcgagca ggctggccga agcgcccgat
     4561 ccggtcgcgc gaatccggct catgatgaag ttatcgttca tcaaatcgag ggcttgggag
     4621 atcgacccgt cgccccgccg ctgctggtcg tctcgattcc cgcggagaaa cgcatcgaga
     4681 aactggacga cggctgcgtt tccagcgggc atcccggccg tttcgggaaa ttgcatcgcc
     4741 caattcaccg ggccgaaggt tgcgccgttc tgcgtgatgg tgtaggaggg aatcacattg
     4801 ctggattgcg ccaccgcgtc atgaatttcc tcggcccaca gccgccggac taatttgcgg
     4861 gcgaacagcg gctcccacgc cgggtcccac gggccgtcgt aacgggccga tagctgatag
     4921 gctcgggagt tcacaatttc gcgcatcagc gccttcaagt cgaacttgct gttgacgaaa
     4981 tcctgagcca gttcctggag gagcgcgggg ttggaaggct gcaacgtcca gggagccggc
     5041 ggcggattgt tcggatcaag ccgggccagg tcaaactggt cgggcggatc cacaatgccg
     5101 cggccaaaaa actgcgccca tatgtaattg acggccgcgc gagcgaactg aaaatcggag
     5161 gtgataaatt gcgccagggc ggcgcgatag ttctgtcccg gcgccggttt cgctcccgtg
     5221 aaccaataga ccggggccac gttcttttcc gtaccgctgg gcgtgccgct cggctgacgc
     5281 gccggccggt tgccagttgt cgtattcaac ggatagttcg cgcgagccgg attgtccacc
     5341 accgaccagg aataaagatt agtctgacct gacattggga cgcgggccac gactgtccgc
     5401 gagacgaacg aagcgaattg ccacgcctga tagcgcgtcc cctggctccc ccagagactg
     5461 agctgatcca ggtgtccgcg gccgttgtgg cacagcaggc agttcatatg agcaatacct
     5521 aaaaaggtgt ccgcaatgtt cgcggcctgc tggtccatga tgtcttgcac agggccgccg
     5581 gtgacgacgc ccccgaccat ccagttcagc tctccctgct ggaagctgtc ggttccctgg
     5641 gcgcttatca gctcgcgcgc catttgatca tagggcttgt tggctgcgag cgagtcgtga
     5701 atgtagttgt aaaacgcatt ccggccttgg gcaaagcgcg ccacctgcga attccggctg
     5761 ttgttctgaa acaggtcccc aaaatacatg gtccacttgt caacccattc cggcttggcc
     5821 agcagttctt ccaccagcgc ttcccgcttg gtgggggacg ggtcgttcac gaaccggatc
     5881 acgcggtcag gcgccggtat gcgcccggtc agatcgagag tgacgcgccg gatgaactca
     5941 aaatcggtcg tcgcaggggc cgggctgacg ccggccgcct gcatggcggc aaataaattc
     6001 cggtcgatcg ggcccatttg ggcgagcggt tgaagggctc cgctgcgcgt cgcggaagag
     6061 agcggcggca gttgccgcac gacctgttgc gtcagagcgc tcaacgagag ctgagaggat
     6121 gaggtcccgg gcatggatag gccctggcgg gcatatcttt catgctgcgg cccgaaatag
     6181 acgcactccg gatgggcgac aggcagttgc ggttcggatt catcctgggc ccggagaacc
     6241 cgcaggcgga agatccccag cggcacagat atcgaaagca gcgcgatcca gaacccgcct
     6301 cttttcattg ctctgatcga cataacggca actcttcatt cccttatgtt gctcctaaac
     6361 ccggccgttg tggaaatgtt tcttttttgg gcaaacgacc ggcccccccg ggttccgtgg
     6421 cgtaacgccg ggcagtttcc ggcatctcgc caaaggcaag tcattgcttt cagtgagttg
     6481 cagccatttt agggaccaca tcgatcctcg ggcgcctggg ccgggcagcg ctccgacagc
     6541 cggagggaaa gcatgggaca acttagcaag cgtgggagcg ccttctccgc gctgcttcga
     6601 aggatttggc tgtctgagcg gcggggccgc ttgggcggag gggcgcagca gcggaaccct
     6661 gccacttgtg cagtatgcgg ctgcgccgat ccggccggac cgcattgcac gtttactctt
     6721 tctttacttt ggcatcgtac ttggacatcc cgtgggcgtg ctggaccgcg aaaatcaggt
     6781 cgtccacttt gcattggtcg gggtcgtact ttactacggc ttcgttgtca ctcagcgata
     6841 tcttgacgtc tttgacgcct cgcgtcgcct ccagtgcatc ctgcaccgtt tcggcgcagg
     6901 aatttcaagt catgccatcg atgaccagcg tgaccgtagc ctccttcggc ggcgcggcag
     6961 ggcttgcgaa cgcaggagcc agaaatgaca gcccggcgac gccgagggcg atgagcatcc
     7021 gtcttctctc catgggaaca cctccggcag gaattgtagc atctccggcc cgcttaacct
     7081 ggagaacttg cgccgtgagg ggaggatgct gatttggcct gcccaggcga ctgccaaaaa
     7141 ggggaagatc gaaagcggaa aaaatgagct taatacgtag cccggccgcc gctcacgtca
     7201 taacactggc cggtgacaaa ggaagcgtcc cggctcgcca ggaagtgagc cacggcggcc
     7261 acttcctcga cggttcccgt gcggcgcatc ggaatccgct cggtcatata atcgacctga
     7321 gccggcgtta gctgatcgag aatgcgggtc cggattaccg ccggcgagat ggagttcacg
     7381 cagatccctt cggtggccac ttccttagcc agggacttag tgaacgcgat gacgcccgct
     7441 ttcgacgccg aataggcgca catgttcgga ttgccctcct tgccggcgat cgaggcgata
     7501 ttcacaatgc gcccgtaccg ctgggcgcgc atgtgaggaa ccaccgcgcg gcaacagtaa
     7561 aaaacgccgg taaggttgac gcggaggacc cggtcccagt cctcatcgct ctgctcccac
     7621 accggagcgg cgcggccgcc aatgccggcg ttgttgacca atacgtcaac gcgccgggtt
     7681 cgttcgagca ccgcgttcac catgcggccg acggaggcat tgtcactgac gtccacgccg
     7741 acgccgaacg cggcgccccc taaatctctc gccgccgcca aagcgccgtt ctcgttgtaa
     7801 tccgccacgg ccaccgtggc gccggccttg gccagccgtg cggcgatagc ccggccgata
     7861 ccttccgccg ctccggtcac aatcgcggtt tgaccctcga gtgaaaggaa catggaagac
     7921 ggctattccg ctttggtgac gaccacctta atggtggtgg aaacgccttt gtgaagattc
     7981 accactgcct catgctcgcc aagcatccgg atgggtttgg caatctcgat tttgtggcgg
     8041 tcgatgttgt agccctgttt ggcaagctct tcggcgatgg ttttcgacgt taccgagccg
     8101 aatagctggt cgttctcgcc ggccttttcg gccacggtta cggtcagctc tttcatcatt
     8161 tcggcgagct tctcggcgtc gctcttcagc ttcgcctcgc ggcgcagggc cgccgctttc
     8221 atatcctcaa taaccttctt gctgccctgc gtggcctcga tggccagctt gcggggcagc
     8281 agataattgc gggcgaagcc gtcggcgacc ttgaccacgt cgccgcgccc gcccagcttg
     8341 ggcacttctg ccttcaatat gacttccata atccatgtcc tcccacgtgg cgagagagcc
     8401 ggcaaccggg ccgcatcagg ccgcgatgcc gccgtctcca ccgttctcgg ctgtctattt
     8461 gccggatcgc tcttttcaca gttctgccgc gaaaggcagc agagcgatat tgcgcgcctt
     8521 cttaattgcg tcgctcaact ggcgctggtg cggcgtgcag acgccggaaa tgcgccgggg
     8581 aatgattctg ccgcgttccg ccacgaagct gctgatcagc ttgacttcct tgtagttgat
     8641 attatcgatc ttctcgacgc agaagcggca cacctttttt ctgcggaagt actgtttttt
     8701 gccgctgggc gcgcccggcg ttccgctctt ttcacccgcg gctccgcgcg gtccgcccgc
     8761 cggtcttctt gcctctgcca tgatggcctc caattctgat tgtattgcga aaagcggcta
     8821 ctctttcacc gccgcttcag cgggaatctg gggagttgct cccggcgacg gcggctccac
     8881 tgcggcaggc gggggtttcc gcagcgcgcg cttctcgcgc ctgctcttga gcttctccag
     8941 gcgcttcaat tcctcgtcga ttcggaccgt cagaaacttg atgaccgtat cgctgacccg
     9001 caaacggcgc tccagttctt tgaccgtttc ggagcccttt tccgtcgaga attgcagcaa
     9061 aacgtaatag ccttcccggt gttttttcac gcggtaggcg agcttccgct tgccccattt
     9121 atcgacctta tccaccacgc cccccgccgc tgtgatcacg tttttcagcg gctcgatgct
     9181 ggcgtcgatc tcttcctccg gcacatcggg ccggataatg aacaggatct cgtaaattct
     9241 cattttccct cggtaatttg gcctccagcg cgacgattga atctcgtcat ggccttagcg
     9301 gcgccttcgg caagtatgga gcgaacggca tcagccccgc ggctgacgag ctcttccact
     9361 tccttctgct gcgaacggcg gaacggcgtg aggtcataat cggccgcgtc caccggccgg
     9421 ccaggatgaa tccccagccg tacccgcgta aaatctttcg ttcccaacgc cccaataact
     9481 gattcaacgc cccgatgccc ggcagaagag ccgcgcagat tgatgcgcag gctcatccag
     9541 ggcaggtcca gctcgtcgta caccaaaatc aggtcggccg gagtcagttt gtatttggcg
     9601 agcaacgcct tgacgggacc gccgcttaga ttcatgtaag actgcggctt ggccagcagc
     9661 acgtccctct gctcaatgac tccgcggcct accagcgact ggcactccgg ccggtgaaca
     9721 ctcaagccgc cgatctgggc cagccggtca atggtcatga agcccaggtt gtgcggcgta
     9781 ttggcgtatt ccggcccggg attgccgagg ccgacaatga gaaacatcga tcgtcattta
     9841 ctgccagtga ccagccagcc atccacagcc tggagcgctt gcgccttact ggttgccatc
     9901 ggagggctga acgattgctc gttacttttt ccctttctcc ttgccttcct tcttctcgcc
     9961 ctcggcggct tcgccttcct ctacctgctt gcccttcttg atcacttcgg gctccgctgg
    10021 agcagcagcg gcggcctcct caaccacggc ttcagccggc ttctcctctt ccttcttcag
    10081 catgatcaca tgaaccagaa tcttttccgg atcgctggtc agccggtagc gctcatcggc
    10141 cggaacatcc ttcacgcgaa tcgcttcgcc gagacccagg tccgtcacgt tgatcgtgat
    10201 gtgttcggga atgttctggg gcaagcattc gacttccacc tcgcgcgtca caatctcgag
    10261 caggcctcct tgcgtcttga cgcccttcgc gtcgccctgt gtgacaatcg gcaccatcgc
    10321 cttcaggctc ttggtgagat cgatgcgcag cagatcggtg tgcaataaat caccccgcaa
    10381 gggatccacc tgccaatcgg cgagcatcgc cgcttcccgc gaaccgcccg caatggccac
    10441 ctggaaaatg gtgttatgcc cggtggcaga ctgcaaaatg tggttgatct gcttggcgtt
    10501 gacactcacc ggaaccgaat ctttaccccc tccatataag acggcgggaa tctggccttg
    10561 ccggcgcagg cgccgagctt cgtttttgcc cctctgcgcc cgcggcgcgg cttcaacggt
    10621 aatatcctta atcaaaacaa acctccaaac aagccaaaac ggcggaatgc ctcgatcaag
    10681 cgcaagcctg tcagcatcgc cgatttgtta attagcgcgc tttcatcggc cgttcctttt
    10741 tcagacgaac atcgttagac gaacatccgg ctgatggacg tctcttcgtg gattgactga
    10801 attgctccgg ccagcagagg agcgacgctc aactgccgga tgcggtcgca ttgccgagcc
    10861 gcctcgctga ggggaatgga atcgctgacg accagttctt tcagatttga tcgcgtgatc
    10921 cgctccacgg ccgagccgga aaggaccgga tgcgtacagc aggcgtacac cgccagagcg
    10981 ccctggccga gcagcgcctc ggccgcgttg accagcgtgc cggcggtatc tacaatatca
    11041 tccacgataa ggcaggtgcg atcccgcacc cgcccgataa tgtgcataat ctcggccacg
    11101 ttcggctgat cgcggcgctt gtcgataatc gccaggggcg ctttcagccg cttggcgaac
    11161 gctcgcgccc gctccactcc gccggcgtcg ggcgagacca cagtcagttt tggcagatcc
    11221 aggtgctgga aatattcaat catcaccggc gtggcgaaga ggtggtccac gggaatgtcg
    11281 aaaaagccct ggatctgagc ggcatgcagg tcgagcgtca agatgcggtc tgccccggcg
    11341 gtcaccagca cgcttgccac gagcttggcc gagatcggca cccgcggctt gtctttccgg
    11401 tcctgccggg cgtagccgta atagggaaga accgctgtgg ttctcttggc tgaggcacgt
    11461 tttaaagcgt ccatcatcag gagcagctca agcaggttct ggtccacggg cgtgcaggtc
    11521 ggctgaacca cgaatacgtc ggcgccgcga acattctcca ggatctgcaa atagatctct
    11581 ccatcggaaa agcgctccac cgaagcttcg gcgcgcggaa ctcccagatg cttgcaaatc
    11641 tcatccgtca gcttcggatt cgcgtttccg gtaaagacct tcaaacggtc gtgttgcatg
    11701 actcggacag gactcgtgac attc
//
LOCUS       DASREK010000018         6484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1331495, whole genome shotgun sequence.
ACCESSION   DASREK010000018 DASREK010000000
VERSION     DASREK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331495"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VFA54_01460"
     CDS             complement(<1..515)
                     /locus_tag="VFA54_01460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS49499.1"
                     /translation="MQAQTENSHILRLERDWGVAEKLCLGVSKLFLKPPLSRLSLRNR
                     ALGRRFETNENYISDRISNIEDYRKLFSGYCSFEGKVVAELGCSTGYLLASFLEYEHF
                     DPIGIDIDPAALDQGRKLHGGTIRFIQSTRTSIPLPDESADVFYCIDTVEHLKRPREI
                     MMECYRVLRPGG"
     gene            complement(598..1023)
                     /locus_tag="VFA54_01465"
     CDS             complement(598..1023)
                     /locus_tag="VFA54_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS49500.1"
                     /translation="MNPLDLRGLRVLIVEDDCDLRSLHTATMRQYGAEAKDAASVAEA
                     IDILLAFRPHVIISDLGMPVEDGFVLIRRIRELAPDQGGTTPAVAVTAYSGSSDRVRA
                     LAAGYQRYMSKPFDPEELVAVVASLVDQPCPSKAESSPR"
     gene            complement(1185..2597)
                     /locus_tag="VFA54_01470"
     CDS             complement(1185..2597)
                     /locus_tag="VFA54_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS49501.1"
                     /translation="MPMDRNILIVEDNEDQRAVLAALTLEWGYKVEIAVDGQDALDKL
                     ASFPAHVIVTDLAMPRLDGAGLLRRLAEQGNKPPTIVMTAYGGDISKAVSMVHDLGAF
                     WFLEKPFDFDAFRVLLARAADHSSLAREKECLQRRLAHSGVLGEMAGRSAAIQRVFAL
                     IQQVAPTKASVLITGESGTGKEMAASTIHKLSPRADGPFVAINCAALPETLMESELFG
                     HEKGSFTGATERRAGCFELAQGGTLLLDEIAEMQLATQAKLLRVLEDGKVRRLGARTE
                     VHVDVRVIAATNKPTDAALDKGELRRDLYYRLNVFRIHLPPLRERKEDIPLIAENLLQ
                     ELNKKHSRRVADISPAVLERLAQYDWPGNVRELRNILERAVILAGEGTLTPAHLPVDF
                     GINPAAPVDGGNGSEKLRRHSDPIALRMGITMAEAEKYLILKTLEHTNNNKTRAADLL
                     GISLKTLHNKLKGYASRPRA"
     gene            complement(2617..5679)
                     /locus_tag="VFA54_01475"
     CDS             complement(2617..5679)
                     /locus_tag="VFA54_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS49502.1"
                     /translation="MHADSIRNEKVKILLVDDTPGNLTALEAVLESLDQELVKVHSGA
                     EALRRILTEDFAVILLDVRMPGMDGLETAALIRQRERSRHTPIIFVTAYRDDEKAIQG
                     YDLGAVDYLIKPIQPEILKSKVAVFIDLAKKNQLLKGHAQLLESKNQELEAAIRQRKE
                     AEEEVRVLNRRLEDRLAELRVVNQELEELYRKVSELDELKTRFFADVSHEMRTPLALM
                     LGPTEKLLESSGLTDEQRRAVETVERHMRAVLKHVNDLLDISKLDAGKMEVNYVNVDL
                     VQVMLLAAANFDELARERGISFSVEAPRSAPAQTDPEKLQRILLNLLSNAFKYVPDGG
                     KVRCELRCTEDKAAMIVADSGPGIPHQLRQIIFDRFRRGDPVAGRRLGGTGLGLAIVR
                     EFVSILGGTVAVGDAPEGGAEFTIEVPLAAPAGIPVSDAAPESGEPEAAAQVLDELHL
                     IHASETEGAPRQHAALEGSPAAEKAAVLVIEDNPAMRNFIGEALSADYRVELASDGEE
                     GLAKAQAARPDLIVSDVMMPRMGAEQLLREIRARRELAGIPVIVLTARVDPELRTALL
                     RDGARDYLTKPFSVTELRLRVRNLIAMKRAREVLQAELESRSQDLEKLAAELALRKRE
                     AESAHAEAEAANRAKDEFLATVSHELRTPLTAVLGWVRLLRTRPFDEATTAQALETIE
                     RNARMQAQLIDEILDVSRIITGKLRLEFRPVDLGRVIEAAIDAVRPVAEANGIQLEVE
                     LDPRASPASGDPGRLEQVVWNLLSNAVKFTPQGGRVEVRLRRIESRAEISVRDTGQGI
                     SPDFLPYVFERFRQADSSHTRSHGGLGLGLAIVRHLVELHGGRVQASSAGVGLGSTFT
                     ITLPLLGRAAAEKLSADRLQMARSPVHIPLGDQRPLDGVRVLLVDDEADTREILGRVL
                     GHCGAQVTAVGSAAEGIEILDREPPDVILSDIQMPDQDGYALIRKVREIEAKRGSFIP
                     AAALTAHVRAEDRIRALSAGFQTHVPKPVEPAELVAVVANLAGRSLRR"
     gene            complement(5682..>6484)
                     /locus_tag="VFA54_01480"
     CDS             complement(5682..>6484)
                     /locus_tag="VFA54_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS49503.1"
                     /translation="LLVVDPRDEAKRELAELLPADRLSATLASSGREALDALRQGCFD
                     CVVIGSAPSDMGSFELIEKMRGGSEGAAAPIVVFQEEDWAEPRQMQEITVSAKPAGDI
                     FEEAVGFLEFASSRLAKSENETTVPARPPRLEISGAKILIVDDDVRNLFALTSALERH
                     GIEVLPAESAKAGIDVLQKTPGVDLVLMDVMMPEMDGYEAIRLIRHMDAFRFVPIVAL
                     TAKALKGDRERCIEAGASDYISKPVDIEELMWLLRAWLPRTRPSSVPV"
BASE COUNT         1273 a         2095 c         1803 g         1313 t
ORIGIN      
        1 ccgccgggcc ggagaacgcg gtagcactcc atcatgatct cccgcggtct tttcaaatgc
       61 tccaccgtat caatacagta gaagacgtcg gccgactcat cgggcagcgg aatcgaggtt
      121 cgcgtcgatt ggatgaaccg gatcgtgcct ccgtgcaatt tccggccttg gtcgagcgcg
      181 gcaggatcga tatcgatccc gatcgggtca aaatgttcgt attccaaaaa cgacgcgagg
      241 agatagcctg tcgagcagcc cagttcggcc actacctttc cttcaaaaga gcagtaacca
      301 gaaaacagct tccggtagtc ctcgatgttc gatatgcggt ccgaaatgta gttttcgttg
      361 gtttcaaatc gccggcccag ggcgcggttt cgcaggctga ggcggctgag aggcggcttg
      421 agaaacaatt tggaaacacc caggcagagc ttctccgcaa ctccccagtc ccgctctaat
      481 ctcagaatat gggaattttc cgtttgagct tgcataggca tccaagacgc acaggccgta
      541 tgcgcgaaga gcaatcgtgc tgccactcgc cacaacccga aatgctctat gcaaaagtta
      601 ccggggactg ctttccgcct tcgacgggca cggttgatca acaagactgg ccaccacggc
      661 aaccaactct tcggggtcaa acggcttcga catataccgc tgataccctg cggccagagc
      721 gcgcacacgg tccgaggatc ccgagtaggc ggtgacggcg accgccgggg tcgtgccgcc
      781 ctggtccggg gccagttccc ggatgcggcg gatcagaacg aagccgtcct caaccggcat
      841 accaagatcg gaaatgatga catggggacg aaaagcgaga agaatgtcaa tcgcttcagc
      901 cacgctggcc gcatccttcg cttccgcccc gtactgcctc atagtggcgg tgtggagcga
      961 tcgcaggtcg caatcgtcct ccactatgag tactcgcaag cctcgtaggt ccaagggatt
     1021 catgcactct attcgttgtg ctgacaacga gctcaggggc ggtggggccc ctgccgcaat
     1081 cgcaaaagcg ccggctttcc ggtctccatc ttccgcgctc cgccacccgg gtcgccaggt
     1141 tcggcggcgc tcgtaatcag ggaccgcaaa aagccgcagc tccactacgc tctcggccgg
     1201 gaagcatagc ccttgagctt gttatgcagc gttttcagac taatgccgag aagatctgct
     1261 gcgcgggtct tgttgttgtt ggtgtgctcc agtgttttca aaatcaagta cttctcggcc
     1321 tcggccatgg ttatgcccat tcgcagcgct atcgggtcgc tgtggcgccg cagtttctct
     1381 gaaccgtttc ctccgtcgac cggcgcagcc ggattaattc cgaaatccac agggagatgc
     1441 gccggcgtaa gcgttccttc ccccgcgaga atgactgccc tttcgagaat gttgcgcaat
     1501 tcgcgcacat ttcccggcca gtcgtactgc gcgagccgct cgagcaccgc cggtgaaatg
     1561 tccgcgaccc ggcgggaatg cttcttgttc aattcttgca gcaggttctc ggcgatcaag
     1621 ggaatatctt ctttgcgttc ccgcagcggc ggcaaatgaa tccgaaacac gttcagacga
     1681 taatagaggt cgcgccggag ctcgcccttg tcgagcgccg cgtcggtcgg tttgtttgta
     1741 gcggctatca cccgaacatc cacatgcact tctgttcttg cgccgagccg gcggaccttg
     1801 ccgtcctcca aaacgcgcag cagcttggcc tgcgtggcga gctgcatctc ggcgatttca
     1861 tcgagcagaa gggtccctcc ctgcgccagc tcgaaacacc cggcccggcg ctcggtggct
     1921 ccggtaaaag atcccttctc gtggccgaat aattcgctct ccataagcgt ctccggcaac
     1981 gcggcgcagt taatggccac aaacggaccg tccgcgcgcg ggctcagctt atgaattgtg
     2041 ctggccgcca tttctttacc cgtcccgctc tctcccgtga taagaacaga agctttggtc
     2101 ggggccactt gctggatcag ggcgaacacg cgctgtatcg ccgccgagcg tcccgccatt
     2161 tcacccagaa ctccggagtg ggcaagccgt ctctgaagac attctttctc gcgcgcgaga
     2221 ctactgtgat cggccgccct cgccagcaat acgcgaaagg cgtcgaaatc aaacggcttc
     2281 tccaaaaacc agaacgcgcc cagatcgtgg accatggaaa ccgctttact gatgtctccg
     2341 ccgtacgcgg tcatgacgat cgtcggcggc ttgttccctt gttcagcgag gcggcgcagc
     2401 aggcccgcgc cgtccagccg cggcatggcc aggtcggtga cgattacatg agcggggaac
     2461 gaggccaact tgtcgagggc gtcctgccca tcgacggcga tctcaacctt atatccccat
     2521 tccaaagtca aggccgcgag aaccgcgcgc tgatcttcat tgtcctcgac gatcagaatg
     2581 ttcctatcca tcggcattag acaacctggc tacctttcag cgccgcaggc ttcgtcccgc
     2641 cagattagcg actacggcca ccagttcggc cggctcaacc ggcttcggca cgtgagtttg
     2701 gaagccggcg gagagagccc gaatccggtc ttccgccctc acgtgcgctg tcagggccgc
     2761 ggcgggaatg aaagatccgc gcttggcttc gatctcgcgg accttccgga tcaatgcgta
     2821 gccatcttga tccggcattt gaatatcgct caatatcaca tcaggcggct cgcggtccaa
     2881 aatctcgatc ccttcggcgg ccgagccgac cgcggtcacc tgggcgccgc aatgcccgag
     2941 cacgcggccc agaatttccc gcgtatcggc ttcatcgtcc actaacagca cacggacgcc
     3001 atcgagcggg cgctgatctc cgagcggaat atggacggga gaacgcgcca tctggaggcg
     3061 atcagcggag agcttctcgg ccgccgcgcg gccgagaagc ggcagagtaa tagtgaacgt
     3121 ggatcctaaa cccacacccg cgctgctggc ctggactcgt cctccatgga gctccaccag
     3181 atgccttacg atagccagcc ccagaccgag acctccgtgc gagcgcgtgt gggaactatc
     3241 ggcctgccgg aagcgctcaa aaacgtaagg cagaaaatcg ggactgatcc cctggccggt
     3301 gtctctcacc gagatttcgg cccgggactc gatccgtctc agacgtacct caacccgccc
     3361 gccctgcggc gtgaacttta ccgcgttgga aagcaaattc cagacgacct gctcgagtcg
     3421 gccggggtcg ccggacgccg ggctggctct gggatccaat tccacctcca gttgaatgcc
     3481 attggcctcc gccacaggcc ggacagcgtc aatcgccgct tcaatgacgc gccccaaatc
     3541 gaccgggcga aattccagcc gcagcttgcc ggtgatgatc cgggatacgt cgagaatctc
     3601 gtcaatcaac tgagcctgca ttctggcatt gcgctcgatc gtctcgagag cctgggcggt
     3661 tgtggcttcg tcaaacggac gcgtccgcag caggcgaacc caccccaaaa ctgcggtgag
     3721 cggagttcgg agctcgtggg acaccgtggc caaaaactcg tctttggcgc ggttggccgc
     3781 ttccgcttcg gcatgggccg attcggcttc ccgctttcgc aaggccagct ccgcagcgag
     3841 cttttccaaa tcctggctgc ggctttccag ttccgcctgg agcacctcgc gggcgcgttt
     3901 catggctatg agattgcgaa cccgcaggcg cagttcggtg acggaaaacg gtttggtgag
     3961 ataatcgcgg gctccgtccc gcaacagcgc cgtgcgcagt tccggatcca cccgggctgt
     4021 cagcacgatg accggtatgc cggccaactc ccgccgcgcc cgtatttccc gaagcaactg
     4081 ttcagcgccc atgcgcggca tcatcacgtc gctgacaatc aagtcgggcc gcgcagcctg
     4141 cgctttggcc agaccttctt ctccgtcaga agccagctcg acgcgataat cggcggacaa
     4201 tgcttcgccg atgaaattcc gcatggccgg attgtcctcg atcaccagga cggccgcttt
     4261 ctcagcggcc ggcgagcctt ccagcgcggc gtgctgcctt ggcgcccctt ccgtttcgct
     4321 cgcatgaatc aggtgaagct catcaagcac ctgcgcggct gcttccggct cccccgattc
     4381 cggggctgcg tccgaaaccg gaatgcccgc cggcgccgct agcggcacct cgatggtgaa
     4441 ttcggcgcct ccttccggcg catcgccaac cgccacagtc ccgccgagta tgcttacaaa
     4501 ttccctgacg atcgccagcc cgagcccggt accgcccaaa cgccggccgg cgacgggatc
     4561 gccgcgccga aagcggtcga atatgatctg gcgcaattga tgcggaatgc cggggccgct
     4621 gtcggccacg atcatagccg ctttgtcttc cgtgcatcgc agctcgcagc gcactttgcc
     4681 gccgtcggga acgtatttga aagcgttgga aagcaggttc agcagaatgc gctgtaattt
     4741 ttccggatcg gtttgggccg gcgcagaccg cggagcctcc accgaaaacg agatccctcg
     4801 ctcccgggcc aactcatcga agttggccgc cgccagcagc atgacttgca cgagatccac
     4861 gtttacgtag ttgacttcca ttttgccggc gtccagcttc gagatgtcca gcagatcgtt
     4921 cacatgcttg agcacggctc gcatgtggcg ctcgacggtc tccacagcgc ggcgctgctc
     4981 atcggtcaaa ccgctcgatt ccagcagctt ttccgtggga ccgagcatga gcgccagcgg
     5041 agtgcgcatt tcatggctta catccgcaaa aaaacgcgtc tttagctcgt cgagctccga
     5101 aaccttccgg tacagctcct ccagttcctg attgacgact ctcaattcgg cgagccggtc
     5161 ctccagccgc cgattcagca ctcgcacctc ctcctctgcc tctttgcgct ggcggatggc
     5221 ggcttcgagc tcttggttct tgctctccag caactgggca tgtcccttga gcaactggtt
     5281 ctttttggcc aggtcaataa agacagccac cttcgatttc aggatttcag gctgaatcgg
     5341 cttgatcagg taatccacgg ctcccaaatc gtagccctgg atagccttct cgtcgtccct
     5401 gtaggcggtg acaaatatga tcggcgtatg ccgcgagcgc tctctttgcc ggatcaatgc
     5461 ggcggtttcc aagccatcca tacccggcat acgcacatcc agaaggatga cggcgaaatc
     5521 ctccgtcaaa atgcgccgca aagcttccgc gccggaatgg accttaacca actcctggtc
     5581 cagactttcc agcacagctt ccagagcagt gagattgcct ggcgtgtcat ccacgagcag
     5641 aatttttacc ttttcattcc ttatggaatc ggcgtgcatc tttacaccgg aacgctgcta
     5701 ggccgcgtcc ggggcagcca ggcgcgcagc agccacatca gttcttcgat gtccacaggc
     5761 ttggaaatgt aatccgaggc gcccgcttca atgcagcgct cccggtctcc cttcagcgct
     5821 ttggcggtca gagccactat gggcacgaat cggaaagcat ccatatgccg tatcaggcgg
     5881 atggcctcgt agccatccat ctctggcatc atgacgtcca tcaacaccag atcaacgcca
     5941 ggcgttttct gcaagacgtc aattccggcc ttcgcgctct cagccgggag cacctcaatg
     6001 ccatgccgct cgagcgcgct ggtcagggca aacagattgc gcacatcgtc atccactatg
     6061 agaatttttg cgccactgat ttccaagcgg ggcggccggg caggtacagt cgtttcgttt
     6121 tccgatttcg ccaaccggga agaagcaaac tccagaaaac cgaccgcttc ctcgaaaata
     6181 tccccggccg gcttggcaga cactgtaatc tcctgcattt gccgcggctc cgcccaatcc
     6241 tcttcttgaa acacaacaat cggcgcggcg gcgccctcgc ttccgccgcg cattttttcg
     6301 ataagctcga aggagcccat atcggaaggg gccgagccaa ttacaacgca atcgaaacat
     6361 ccctgtctca aagcgtccag cgcttcccgc ccgctgctgg cgagagtagc ggacagccgg
     6421 tctgcgggaa gcaattccgc cagctcgcgt tttgcttcgt cccgggggtc cacaaccagc
     6481 aatt
//
LOCUS       DASREK010000019        17049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2320223, whole genome shotgun sequence.
ACCESSION   DASREK010000019 DASREK010000000
VERSION     DASREK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17049
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320223"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..777
                     /locus_tag="VFA54_01485"
     CDS             67..777
                     /locus_tag="VFA54_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49504.1"
                     /translation="MTIGAERNKVIALCALLAVAGATWYYQFSSFPETPPAPPSRASA
                     RAPGAQAQARQVRASAEDLLDSRTGPRTRSGSNEFKPSLKAGKPGEGPDPDKVDPTLR
                     TDLLAKVQAVQYQGVERNLFQFGAPKPKPPSPAQIAAAKKEAEAAAKKIAEASAPASP
                     PKPAAPPINMKYFGYANRPGDSRRRAFLLDGDDILVIYEGEIIKKRYKVIRIGISSLV
                     VEDLEFHSEQTLPLQENG"
     gene            781..2445
                     /locus_tag="VFA54_01490"
     CDS             781..2445
                     /locus_tag="VFA54_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49505.1"
                     /translation="MTRRARKREEGYALLVVLFLGALIAIGLALSLPRAAFEAQRARE
                     DDLIYRGSQYSRAVQLYFRKFRRYPASMDDLEKTNNIRFLRRKYIDPITQKDEWRIVH
                     IGPGGVFTDSLVYDRPRQNQNNQDGGTAAGSVSGPPSPADPGQITYFNQGQPNAGQQI
                     TGMADRLRASNAGTTPADPNAPPGSQGNPYGAQNYNYPGQPGYGNPSQSGMPGQFYVP
                     GQSGMPAGTQAGIGSAISGTGYTGAVPGAATNPPFGTPAGFPAGLGTAQQGPYAGASP
                     VAGTPAGYQAGAVTGSPAVGPAVGSEAARIIGQLLTTPRPGGLAGLMGAGPQPVANPF
                     GGPSASPFGGPGASSSLGGGSAFNNPGASSFQGGSSAGGGTTGFGGGIAGVASKSEAR
                     GIKVYRDRETYNEWEFVYDYRRDPLLVGGAAAGTGGLPSQPGTASQSGTASAFGQGGA
                     STSGQSSVFGQSSFGQPGGQTGQPSSGQPGGQPFGQPGFTTPGQMPNTSINPTQPGLS
                     PTPIGPPRLPIQGPPLAIPNSPPQDQPPAQQQPAPVPEQNPPPPQP"
     gene            2539..3543
                     /locus_tag="VFA54_01495"
     CDS             2539..3543
                     /locus_tag="VFA54_01495"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HZS49506.1"
                     /translation="MGSIPPFAVECDNTWACKTTRRNDMTCSIIARDPATGELGIAVQ
                     SRYFAVGRIVPWIEAGVWAVVSQSFANPVYGHETLRLLRSGLEPQHALKKLLSEDPGE
                     AMRQVAILDAQGRIAEHTGTQCVPAAGHAAGANCCAQANMMAKNTIWNAMVQAFENAS
                     GELADRLLAAMEAAEREGGDVRGRQAASLIVVSGKPSGISRLDHSVDLRVDDHPDPVA
                     EIKRLLRYSRAHERANRAINKAVANDLSAALADLDVCCESYPNEPEFLIRRVLVLIPL
                     GRIGEALQTLRQAYAIHPGWSELLLRFADAKVIPIRREMLEPLVASVTSENQGSAIRK
                     "
     gene            complement(3693..4322)
                     /locus_tag="VFA54_01500"
     CDS             complement(3693..4322)
                     /locus_tag="VFA54_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZS49507.1"
                     /translation="MIPGKAEDSEPRLAGLILAAGESSRMGSDKALLDYGGSTFLETL
                     INLFLRRVFPVVVVLGHNAAKIQAALAPRPGLIVAVNSNYKAGQLSSLQTGIRALPRG
                     CEGALFTLVDHPAVADATIAAMIRRFAGSNLPLVVPCHQGRRGHPVLLSRLLLDEIAA
                     LPVSGSAKEVIRAHLDIGAVLDVDDPGILRDIDTPEDYRDLKASGSPGC"
     gene            complement(4319..5158)
                     /locus_tag="VFA54_01505"
     CDS             complement(4319..5158)
                     /locus_tag="VFA54_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HZS49508.1"
                     /translation="MDVFEELVRLRRLGQKSALATIVDVRGSIPSFESAKLLVREDGS
                     MVGTIGGGCVEAEVWNAAREVIETEKPRRLSFNLGQDAAYDNGLICGGQLDIFIEPVL
                     PIPSAYIFGAGHISKSLSKVASLAGFRTVVIDNREAFANRERFPDVDDVIAAEYEEVF
                     PKLEINESSYLIIVTRGHRDDMRVLRWAIEQPARYIGMIGSKRKTIAVIKEFEKEGVP
                     REKFERIHSPMGLEIGAITPEEIAVSVVAEMIDIRRNAQAGWNPLSKSVFALGLLKER
                     QAK"
     gene            5233..6261
                     /locus_tag="VFA54_01510"
     CDS             5233..6261
                     /locus_tag="VFA54_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZS49509.1"
                     /translation="MKAVRIHEHGGPEVLRYEDVPDPAPGPGEVLIRVRACALNHLDL
                     AVRRGIPGQTIPLPHILGSDVAGEIAALGPGAGRVKVGDRVLLAPARSCRQCVECVSG
                     RDNFCRQYRLLGYFIDGGNAEYITTPDVYVLPIPGDLNFIQAAAVPLVFLTAWHMLVG
                     RAHIRRGEDVLVIGASSGVGSAAVQIAKLQGCRVIATAGGEVKLALARELGADAVLDH
                     YRQDIAAEVKRLTARRGVDVVFEHVGEATWRQSVASLAYGGRLITCGATTGGRGEIDI
                     RALFWKQISLLGSFMGTLGELHEVLASIGRKELRPVIDKVFPLAGVRAAHERLEKKEQ
                     FGKVVLTP"
     gene            6446..7972
                     /gene="aroE"
                     /locus_tag="VFA54_01515"
     CDS             6446..7972
                     /gene="aroE"
                     /locus_tag="VFA54_01515"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZS49510.1"
                     /translation="MAYRSEFPPVCVALGCAERERLDRSAAHACDSGEEFLEIRLDML
                     EDPREGIGTIRRIKRRYPDVFLLATCRRKRNGGGFEGTIEEQLELLGAAIEAGAQGVD
                     IEIETAAEAPERVAGLRERARVVVSYHNFQFTPPLAPILRRLEKAPADVYKLAAMARK
                     PSDNWRLLDVARRYQNHPLVVVGMGETGVPSRILSLSRHSAFTYAAPPWDSAGKNALP
                     GTAPGQLSSSLLHRQYRAGRHTCATAVYGVIGNPVRHSISPAVHNRAFQARRIDAVYV
                     PFLVEEGRLRDFFQLAARLPVAGFSVTIPHKQRVLRYLDAVDPAARRIGAVNTVYRKQ
                     GKLRGANTDAAGVTAPLEKRLKLKNSKILIAGSGGAARGAAFALAEKGAQVFLTGRNV
                     NRVRALARACGAEAVENERLAGRRFDVLVHATPLGMRPRIDECYFPDEIPAELVFDMV
                     YNPLETRLLKKARAAGKTVISGLEMFLEQAALQFEIWTGTRAPRLAMETTAREALETS
                     "
     gene            7975..9456
                     /gene="trpE"
                     /locus_tag="VFA54_01520"
     CDS             7975..9456
                     /gene="trpE"
                     /locus_tag="VFA54_01520"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZS49511.1"
                     /translation="MLPDLKTFLAQAREGNVVPVYKIVTADLETPVSAYLKLASSERY
                     SFLLESVEGGEKIGRYTYLGTSPFLVVKAFGDRIEITRQGRTRLQNGNLFLALRELTR
                     QFHPVRQQGLPPLSAGAAGYIAYDLVRQIEPKVPPFAKDDLKTPDAVFLFFSTLLAFD
                     HVKHQIWIIANVRCDEWAGKLEKGYCHALGEIAAIEKSLIRNLPRRKSAGMRGGPPQP
                     LSNIGKERFCRSVEKAKEYILAGDIFQVVLSQRLDLKLRAEPFQIYRALRTVNPSPYM
                     FYLKLDRDIVLGSSPEMLVKVDGRNVEYRPIAGTHPRGASEAEDQEIERRLLADEKER
                     AEHIMLVDLGRNDLGRVCEYSSVQVKDLMVLERYSHVIHIVSRIAGKLRPEADAFDTL
                     MACFPAGTLSGAPKVRAMEIIAELEPTRRGVYGGSVMYLDFSGNLNSCIGIRTMTVRG
                     RDAHVQVGAGIVADSVPEREWEETMNKAGALLRAVELAESGEF"
     gene            9456..10037
                     /locus_tag="VFA54_01525"
     CDS             9456..10037
                     /locus_tag="VFA54_01525"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZS49512.1"
                     /translation="MILVLDNYDSFTYNLVQYLGELGAELKVVRNDATTVAEVRRLRP
                     AGIVISPGPCTPREAGISVPVIRELAGEIPILGVCLGHQAIGEAFGGRVVRAPYLMHG
                     KTSQIHHDGKTIFAGLPQDFIATRYHSLIVEKESLPGELEISATTADGLIMGLRHRKF
                     TVEGVQFHPESVLTGTGKAILQNFLRLRQPAAA"
     gene            complement(10095..11402)
                     /locus_tag="VFA54_01530"
     CDS             complement(10095..11402)
                     /locus_tag="VFA54_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZS49513.1"
                     /translation="MADSITVSELSILQDQPQPSDLDRCVHCGLCLNACPTYRELGLE
                     MDSPRGRIYQMVQVANGRMDVSDSYVEHIELCLACRACETACPSGVEYGKLVEAARAH
                     IEAVRPRPLLARLLRRFVYGRLLVSPGLMQAAGNLVALYQRSGLQRLVRSTGLLKPLG
                     RLDKLERLMPPAERPFFFDEIGKTFPARGARRRRVALLAGCLANVSFARLNEATVRVL
                     QENGCEVDVPAGQTCCGALHVHAGLRDLGRRLARRNIDVFLAGGYDALITNAAGCGST
                     LKEYGELLEHDPQYREKARLFASQVKDVNEFLGSIELSRRMAPLNIIATYQDSCHLAH
                     GQRVRVQPRRLLEAVPGLQFRELPLSDLCCGSAGVYNVQHTEMSMRLLEKKMQMAAAT
                     GADTIVTANPGCMLQLRAGVEMFGRGQRVMHVVEVLDEAYRKA"
     gene            complement(11377..12576)
                     /locus_tag="VFA54_01535"
     CDS             complement(11377..12576)
                     /locus_tag="VFA54_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZS49514.1"
                     /translation="MQKLQPADIGELKEVLAHCNAARKSIEIGGHFTKRWMGGSVQPA
                     DMVVSTARLNHVIDYEPRDLTISVEAGVGFRNLQELLRENNEMLPLDPPLAEQSTVGG
                     VVAASCSGPRRRLYGSARDMVIGMTFVTVEGRPVRSGGMVVKNVAGLDMAKLMIGSFG
                     TLAAIASVNFKVFPRPPKEKTFVLAFQSLERALQARDAVLRSALQPSVIDLANPALCR
                     RLGLKLGQGFWLLVEAGGIDAVLGRYERELKALARQLETAEFVSLEADEAESLWRSVR
                     DFPALPPLNGSSETGPRETGAVVRISTPLARLDQAFAAAGDWPALARAGTGVTYVRCE
                     ESPAELTGRARAAGLYAVIESCSTGDKDNLELWADPGPELDLMSRIKNNLDPQSILNH
                     GRLYNRL"
     gene            complement(12576..13433)
                     /locus_tag="VFA54_01540"
     CDS             complement(12576..13433)
                     /locus_tag="VFA54_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49515.1"
                     /translation="MPRFAQVWLYSVAILVACALLASAAEQKKPLRIVRAALLEAEDG
                     FSASPDAEYHGGETLYMAFHVEGFTVDRSDRVKLSYRIDTLDPAGTPIVEPQSGKVDA
                     ELAPEDAKWTPRVRFSATLPEWVESGLCRFVIHVVDDFTRQQVSAELPFRVQGRAVAP
                     SDSLVVRNFQFAREENGDPLPAPAFRRGDALWASFDITGYKTGNNNLVQVQYELSVFN
                     AEGKLIFRQPQPAEEKGTSFYPRRYVHAAFNLNLEKSIAPGEYTIVLAARDEIGQQTS
                     ESRHSFTVE"
     gene            complement(13402..14634)
                     /gene="thiI"
                     /locus_tag="VFA54_01545"
     CDS             complement(13402..14634)
                     /gene="thiI"
                     /locus_tag="VFA54_01545"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HZS49516.1"
                     /translation="MPVLVLHYHEMWLKGRNRNFFLHKFTESIRSHLDGLGPLRIVHE
                     DGRVLLHLESEEALRGAARRIPRIFGVAHYSVAEEVERDLAQVEQAAWKQLAAQSFDT
                     FAIRVRRSDKTLPFRSQEAEKRIGRYVLDAAQAAGRRIRVDLDHPEATCFAEFTGHRA
                     LVFSEKMPGPGGMAANTAGKLVCLLSGGYDSAVAAYLMMKRGARVIFVHFHGVAARVG
                     EASEPVAREIVRTLAPYQLGARLYLIPFHELQREALLQAPEEYRILIYRRLMLRIAER
                     IAHRRHALGLVVGDSLAQVASQTLQNMAAVEAAIRLPVYRPLVGFDKREILDLARRIG
                     TYETSSERFADCCPAFLPRSPRLYASGDELDRAESKLDIPSMVARGVAGARRETYRFA
                     NGRVMEQSDAPIRAGMAL"
     gene            14770..15444
                     /locus_tag="VFA54_01550"
     CDS             14770..15444
                     /locus_tag="VFA54_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS49517.1"
                     /translation="MPSILVIDDDETVRDTIGIMLEQEGFRAQLAADGREGFEKALVQ
                     KPDLILVDLRLPGMNGMEICRQIRAAQLSTPIIVLSAVGDEIDKVLLLEIGADDYVTK
                     PFGSRELLARIRAVLRRASPEGKKILRFSDVEVDLERRVVMRRSEPVKLTPSEYNLLV
                     YFLQNPDRPLTRDMILNSVWGYDTFPNTRTVDIHVVKLRQKLEPDPNIPRHFTTVHGV
                     GYRFLP"
     gene            15631..16008
                     /locus_tag="VFA54_01555"
     CDS             15631..16008
                     /locus_tag="VFA54_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS49518.1"
                     /translation="MTRNILIVEDAETTATTLEIALLNLPNTNVVRAPDGLRALEYLA
                     PESGHTIDALVTDLEMPFLDGFELIRRVREQKRFAMLPIVVISGRTDAQTLENVMKLG
                     ANAYFAKPCSALAIQKKILDLLP"
     gene            16005..>17049
                     /locus_tag="VFA54_01560"
     CDS             16005..>17049
                     /locus_tag="VFA54_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49519.1"
                     /translation="MKMFRMEKLAFSLAALLAVPGRAQTPTSMDAIGQRLERLEQQNA
                     ELLSEVRLLRQELESLKASGGAAHESARALNPEAAAPESERLERLEERVDLQEGRLNE
                     QDAVKVESTQRVPVRLTGMALFNAFKDTSHGGGADNPVVAAPAPGPINAGATLRQSVI
                     GLEFDGPEAIAGAKIRGSFLMDLFAGTSGILGSQARLRTASIEARWETRGFLVGQEKP
                     IFSPREPNSLAQVGTSPLTAAGNLWLWRPQIRFEQRVELGRKTDLTARLGVFETGENG
                     AALDPQFAPTLEQRRPALQGRFQLAHRFDENRRIEIAPGFHVSTTHVAASSVPSNVVS
                     VDWFVNPIRRLEFT"
BASE COUNT         3314 a         5336 c         5350 g         3049 t
ORIGIN      
        1 cgggcgccaa gctgagcgtt accctcaagc tcgacgcctt cgtgaaagag tcgtgagggc
       61 cggatcatga caatcggcgc ggagcggaac aaagtaatcg cgctatgcgc gttgctggcc
      121 gtggccggag cgacttggta ttaccagttt agttcgttcc cggagacgcc gccggctccg
      181 ccttccaggg cttcggcccg cgctccgggc gcccaggcgc aggcgcgcca ggttcgggcc
      241 tccgccgaag atcttttaga cagccggacg ggaccgcgga cccggtccgg cagcaatgag
      301 ttcaagccct cgctgaaagc cggcaagccg ggtgaagggc ccgaccccga caaagtggac
      361 cccacgctgc gcactgacct cctggctaag gtgcaagccg tacagtatca gggcgtcgag
      421 cgtaatctgt tccagttcgg cgcgcccaaa cccaagccgc cctcccccgc gcagattgca
      481 gccgccaaaa aggaagccga ggctgcggca aagaagatag ctgaggcatc cgccccggca
      541 tccccgccga agcccgccgc tccgcccatc aatatgaaat actttgggta tgcgaaccgg
      601 ccgggggatt cccgccggcg ggcgttcttg ctggatggag acgacattct ggtgatctac
      661 gagggcgaga tcatcaaaaa gcgctacaag gtaatccgca tcgggatcag ctccctcgtg
      721 gtcgaggacc tcgagttcca cagcgagcag accctgccgc tccaggagaa cggctgagcg
      781 atgaccagac gtgccagaaa gcgggaagaa ggctatgcgc tgctggtagt gctgtttctt
      841 ggagcgctca tcgccatcgg tctggcgctt tcgctgcccc gcgcggcctt tgaagcgcag
      901 cgcgccagag aggacgatct gatttatcgc ggcagtcaat attcgcgcgc cgtccagctc
      961 tacttccgta aattccgccg ctatccggcc agcatggacg acctggaaaa gacaaacaac
     1021 attcgcttcc tgcggcgaaa gtacatagac ccgatcaccc aaaaagacga gtggcgcatt
     1081 gtccacatcg ggccgggagg tgtattcacg gattcgctgg tctacgaccg ccccaggcaa
     1141 aatcaaaaca atcaagacgg cgggacggca gccgggtcgg tatctggacc gccctctccg
     1201 gccgatccag gccagataac gtatttcaat caaggccagc cgaatgccgg ccagcagatc
     1261 accggcatgg cggaccggct gcgcgctagc aatgcgggaa ccactcccgc cgacccgaac
     1321 gcccctcccg ggagccaggg aaacccctac ggtgcgcaga actacaacta tccggggcag
     1381 cccggttatg gcaacccgag ccagtccggc atgcctggtc agttttatgt gccaggacag
     1441 tccggcatgc ccgccggaac gcaggcaggc atcggaagcg caatctccgg cacggggtat
     1501 accggagccg ttccgggcgc cgccacaaac ccgccgttcg gcacgccggc cgggtttccc
     1561 gccggtttag gcacagccca gcaggggccc tatgccgggg cctcccctgt agcggggacg
     1621 cctgccggat atcaggcggg ggctgtcacc ggaagccccg ccgtcggtcc tgccgtgggc
     1681 agtgaggcgg caaggattat cggccagctt ttgaccacgc ctcgtccggg cggtttagcc
     1741 gggttgatgg gtgcaggccc gcagcccgtg gccaacccgt ttggcggccc cagcgccagc
     1801 cctttcggcg gccctggcgc gagctcatcg ctgggcggcg gtagcgcgtt taataatcca
     1861 ggcgccagca gctttcaggg cggcagtagc gcgggcggag gaactacggg tttcggcggc
     1921 ggcatagccg gcgttgcgag caaatcagag gccaggggca tcaaggtcta ccgggaccgg
     1981 gaaacttaca acgagtggga attcgtttac gactaccggc gggatcccct gctagtaggc
     2041 ggcgcggcag cgggaactgg cggcttgccc agccagcccg gaactgcgag ccaatctggg
     2101 acagcttccg ccttcgggca gggcggcgcc tctacatccg gtcaaagctc tgttttcggc
     2161 caatcctcat ttggacaacc tggaggacag acgggacagc cctcttccgg ccagcccggc
     2221 ggtcaaccgt tcggccagcc cggattcacc actcctggac aaatgccgaa cacctccatc
     2281 aatcccacgc agcccggatt gtccccgacg ccgattggac cgccgaggct gcccattcag
     2341 ggcccaccct tggcaattcc gaattcaccg ccccaggacc agcctccggc ccagcagcag
     2401 ccggcgcctg ttcccgaaca aaatccccct ccaccccagc cttaggctgt gtcgatagtc
     2461 cgcggttccc caaagtggac ccaggttctg attggagctg cgggacctgg gctgcggcgg
     2521 ttcgtccaaa gcgcggccgt gggctccata ccgccctttg cagttgaatg cgacaatacg
     2581 tgggcctgca aaacaacccg caggaatgat atgacctgtt caatcatcgc acgggatccg
     2641 gctaccggag aattgggaat cgctgttcaa tcgcgctatt ttgcagtagg gcgaatcgtg
     2701 ccctggatcg aagctggagt gtgggccgtc gtatcgcaat cgtttgccaa cccagtttat
     2761 ggacacgaga ctctgcgcct tctgcggtcc ggcctggagc cgcagcacgc acttaagaaa
     2821 ctcctgagcg aggacccggg cgaggccatg cgccaagttg caattctcga cgcacaagga
     2881 cgcattgctg agcacacggg gacacagtgt gttccagcag ccggccatgc tgctggtgcg
     2941 aactgctgcg cccaagccaa catgatggcg aaaaatacga tctggaacgc gatggtgcaa
     3001 gccttcgaga acgccagcgg cgaactagcg gatcggctgc tggccgcgat ggaagcagcc
     3061 gagcgggaag gcggggatgt gcgcgggaga caggctgcct cactcatcgt cgtctctggc
     3121 aagccctccg gcatatccag attggatcat tccgttgacc tgcgtgtgga cgaccacccc
     3181 gaccctgtgg ctgaaataaa acggctgttg cgttactcgc gcgctcacga gcgagcgaat
     3241 cgggcgatta acaaggctgt ggcaaacgac ctctccgctg ccttggccga tctggacgtt
     3301 tgttgcgagt catatcccaa cgaaccagag tttctcattc gccgtgtgct ggtcctgatc
     3361 cctctgggga gaattggcga agctctccag acattgcggc aggcatacgc tattcatcct
     3421 gggtggagcg agctcttgtt gcgcttcgca gatgcgaaag taatcccaat ccgccgcgaa
     3481 atgctggaac cgctggtggc aagcgtcacc tcagaaaatc agggctctgc aatccgcaaa
     3541 tgaaacaacg gctgcggcct ggcaggcgcg cggacggccc tttgaccgct cggcaatgag
     3601 ccgcgccggg gcggttccga cggcgccgcg ggccgaacag tttggcctgc gacagacgcc
     3661 acggaaatat ttggctttta gagttcgtca ggtcaacaac ccgggctgcc ggaggctttc
     3721 aaatcacggt aatcctcagg cgtgtcgata tcgcgcagaa tgccaggatc atccacgtcg
     3781 agcaccgctc ctatgtcgag gtgcgcccgg atcacttcct tggcgctccc cgaaaccggc
     3841 aaggcagcga tctcgtcgag caggagacgc gaaagcaaca cgggatgccc gcgccttccc
     3901 tgatgacagg gaaccaccaa cggcaaattc gatccggcaa accggcgaat catggcggca
     3961 atggtggcat cggcgaccgc cggatgatcg accaaagtga acaaagcgcc ctcgcagccg
     4021 cgcggcagcg cccggatgcc ggtttgcagc gacgacagtt ggccggcttt gtaattcgag
     4081 ttgacagcga cgatcagacc cgggcgcggc gcaagagcgg cctggatttt agcggcgttg
     4141 tggcccagca cgaccaccac gggaaaaacg cgccgcagga agagattgat tagagtttcc
     4201 agaaaggtcg agccgccgta gtcgaggagc gccttgtcgc tccccatacg gctcgactcg
     4261 ccggccgcca gaatcaagcc ggccagcctt ggctcggagt cctctgcctt gcccgggatc
     4321 acttcgcctg gcgttccttg agcaggccga gggcaaacac cgatttcgaa agaggattcc
     4381 agcccgcttg cgcattgcgc cggatatcga tcatctcggc gacaacgctg acggcgattt
     4441 cttccggcgt gatggctccg atctcgaggc ccatgggcga gtggatgcgc tcgaatttct
     4501 cccgcggcac gccctctttc tcgaactcct taatcaccgc gatggttttg cgcttgctgc
     4561 cgatcatgcc gatgtaacgc gccggctgct cgatggccca acgcaggacc cgcatgtcgt
     4621 cgcggtggcc gcgcgtgacg atgatcaggt acgaagactc attgatctcg agcttgggga
     4681 aaacttcctc gtactcggcg gcgattacgt catccacgtc cgggaagcgc tcgcgattcg
     4741 cgaatgcttc gcgattatcg atgacaacgg tccggaagcc ggcgagagaa gcgactttgg
     4801 agaggctttt cgaaatatgt ccggcgccga agatgtaggc cgatggaatg ggcagcacgg
     4861 gctcgatgaa gatatcgagc tggccgccgc agatcagacc gttgtcgtag gcggcgtcct
     4921 gcccaagatt gaagcttagg cgccgcggct tctcggtctc gatcacctcg cgcgcggcat
     4981 tccatacctc agcctccacg cagccgccgc cgatcgtgcc gaccatcgag ccgtcttccc
     5041 gcaccagaag cttggcgctc tcgaagctgg ggatggaccc tcgcacatcg acaatggtcg
     5101 ccagggctga tttttgacca aggcgacgca agcgcaccag ttcctcgaaa acgtccatat
     5161 cgatgccaat tataatatgg gctggcccgt gcaggcgaaa gcagcactgc ccgcctggaa
     5221 ggcggcttcc tgatgaaagc ggttcgcatc cacgaacatg gcggcccgga agtgttgcgc
     5281 tatgaggatg ttcccgaccc ggcgccgggg cctggggaag tgttaatccg ggtccgagcc
     5341 tgcgctttga atcatctcga cctcgccgtg cgccgcggca ttccgggcca gaccattccg
     5401 ttgccgcata ttttgggcag cgacgtggcc ggcgagattg ccgctctcgg gccgggcgcg
     5461 ggaagggtca aagtgggcga ccgcgtgctg ctcgctccgg cgcgaagctg ccggcagtgc
     5521 gtggaatgcg tatcgggacg cgacaatttc tgccgccagt accggctgct gggctatttc
     5581 atcgacggcg gtaatgccga gtacatcacc acgcctgacg tctacgtcct tcccatccct
     5641 ggcgacctca attttataca ggcggcggct gtgcccttgg tcttcttaac ggcctggcac
     5701 atgctggtgg gacgcgccca catccggcgc ggagaggatg tgctcgtgat cggggcctcg
     5761 agcggcgtcg gcagcgcggc cgttcagatc gcaaaactgc aaggctgccg cgtgatcgcc
     5821 accgccggcg gagaggtgaa gctggctctg gcgcgcgaac tgggcgcaga cgccgtgctc
     5881 gaccattacc gccaggatat cgccgccgaa gtcaaacgcc tgaccgcccg ccgcggcgtc
     5941 gatgtggttt tcgaacatgt aggagaagcc acctggaggc aatcggtcgc atcgctcgcc
     6001 tatggcggcc ggctgattac ctgtggagcg accaccggtg gccgggggga gatcgacatt
     6061 cgcgcgctct tctggaagca gatctctctt ttgggctctt ttatgggaac gctgggagag
     6121 ctgcacgaag tcctggcgtc aatcggacgg aaggagcttc gtccggtgat cgacaaagtc
     6181 tttcctctgg ccggcgtccg cgccgcacac gagcggctgg aaaagaaaga gcagttcgga
     6241 aaagtcgtgt tgactcccta atacctggtt cctatcgagc ttaacatccg gcgaagcccg
     6301 tccaaagcaa gaagctgcgg ccgccggcgg agcgagaggg ttccaccggg gtagttcgcg
     6361 aaccagccga cgcgcctttt cccggctcgc gggtctctgg cgcgtaaagc gcggcgaaaa
     6421 tgctatcctg agtctcaccc ctgacatggc ttatcgaagt gagttcccgc cagtctgcgt
     6481 agccctgggc tgtgcggaac gcgagcggct ggacagatcg gcggcgcacg cttgcgacag
     6541 cggcgaagag tttctcgaga ttcgtctgga catgctcgag gatccccgcg aaggaatcgg
     6601 tacaatccgc cggatcaaga ggcgttaccc cgatgtattc ctgctggcca cgtgccgccg
     6661 gaaacggaac ggggggggct tcgagggaac gatcgaggaa cagctcgaac tactcggcgc
     6721 ggcgatagaa gccggggcgc agggcgtaga tatcgaaatc gaaaccgctg cggaagcccc
     6781 ggaacgggtg gccggcctgc gggagcgggc gagagtcgtg gtctcctatc acaattttca
     6841 attcacgcct ccgctcgcgc cgattctccg gcggctcgag aaggcgccgg ccgacgtcta
     6901 caagttggcg gcgatggcgc gtaagccgag cgataactgg cggctgttgg acgttgcgcg
     6961 ccgttatcag aatcatccac tggtcgttgt cgggatgggc gagaccggcg tgcccagccg
     7021 catcctttcg ctttcgcgcc attcggcgtt cacctacgcc gcgccgcctt gggactcggc
     7081 cggaaaaaac gcgcttccgg gcacggcccc ggggcagttg tcgagctccc tgctgcaccg
     7141 ccagtatcgc gccggccgcc acacttgcgc gacagccgta tatggagtga tcgggaatcc
     7201 ggtgaggcac tccatttcgc ccgcggtgca taaccgcgcg ttccaggccc ggcggatcga
     7261 cgccgtctat gtgccgtttc tggtggaaga agggcggctg cgcgattttt ttcagctcgc
     7321 cgcgcgctta cccgtggccg gtttcagcgt gacgatacct cacaagcaga gagtcctgcg
     7381 ctatctcgat gccgtagatc cggcggcccg gcgcatcggc gctgtcaata ccgtctaccg
     7441 caagcagggg aagctgcgcg gggccaatac cgacgccgcc ggcgtgactg cgccgctgga
     7501 gaagcgctta aaattgaaga attcgaaaat tctgatcgcg ggaagcggtg gagcggcccg
     7561 cggagccgcc tttgctctgg ccgaaaaggg cgcgcaggtg tttctgacgg ggcgaaacgt
     7621 aaaccgggtc cgggcgctgg cgcgcgcctg cggcgcggaa gctgtggaga acgaacgcct
     7681 ggccggccga cggttcgatg ttcttgtgca cgctacgccg ctcggcatgc ggccgcgaat
     7741 cgacgagtgc tactttccgg acgaaattcc cgccgagctg gtcttcgaca tggtctacaa
     7801 ccctctggaa acccggctgc tgaagaaagc ccgcgctgcc ggcaagacgg tgatttcggg
     7861 gctggaaatg tttctcgagc aggcggcctt gcaatttgaa atctggaccg gcacgcgggc
     7921 gccgcgcctt gccatggaaa caaccgcgcg ggaggctctc gaaacgagct gattgtgctg
     7981 cctgatctca aaactttttt ggcgcaagcc cgcgaaggca acgtcgtgcc ggtctacaag
     8041 atcgtgacgg cggatctcga aacgcctgtc tcggcttacc tgaagctggc gtcgtcggaa
     8101 cgctactcct tcctgctgga aagcgtcgaa ggcggcgaaa agatcggccg ctatacctac
     8161 ctggggacca gtccgtttct ggtcgtgaaa gcctttggcg accggattga aatcaccagg
     8221 cagggccgga cccggctgca aaacggcaat ctgtttctgg cgctgcggga gttgacccgg
     8281 caatttcatc ccgtccggca gcagggcctg ccgcccctga gcgccggggc ggccggctat
     8341 atcgcctacg acctggttcg ccagatagaa cccaaagtgc cgccctttgc gaaggacgat
     8401 ctgaaaacgc ccgacgccgt attcctgttt ttttccactt tgctcgcgtt cgatcatgtg
     8461 aagcatcaga tctggatcat cgcgaatgtg cgctgcgacg aatgggccgg gaaactcgag
     8521 aaaggctact gccacgctct cggcgaaatc gcggctatcg aaaaatcgct gatccgaaat
     8581 ctgccccgcc ggaaaagcgc ggggatgcga ggcggcccgc cgcagccgct ttccaatatc
     8641 ggcaaagagc gattctgccg gtccgtggaa aaggccaagg agtatatact ggcaggagat
     8701 atttttcagg ttgttctttc ccagcggctc gatctgaagt tgcgggccga gccgttccag
     8761 atctaccgcg cgctgcgcac cgttaatcca tcgccgtaca tgttctattt gaagctggat
     8821 agggacattg ttctcggaag ctcgccggaa atgctggtca aggtggacgg acgcaatgtc
     8881 gaataccggc cgatcgccgg cacccacccg cgcggagcca gcgaggccga ggatcaggaa
     8941 atcgaacgcc gcctgctggc cgacgagaag gagcgcgccg agcatatcat gctggtggat
     9001 ctcgggcgca acgacctggg gcgggtgtgc gaatactcgt cggtccaggt gaaagacctg
     9061 atggtcctgg agcgctattc ccacgtgatt cacatcgttt cgcggatcgc cggcaagctg
     9121 cggccggaag cggacgcatt cgatacgttg atggcgtgct ttccggccgg cacgctctcg
     9181 ggcgctccca aggtacgggc catggagatc atcgccgaac tggagcccac ccgccgcggg
     9241 gtttacggcg ggagcgtcat gtacctggat ttttccggca atctcaattc gtgcattggc
     9301 atccgcacga tgaccgtgcg cggccgcgat gcgcatgtgc aggtcggagc cggcattgtg
     9361 gccgattcgg tccccgagcg ggaatgggaa gagacgatga acaaagccgg agccttgctg
     9421 cgagccgtgg aactggccga gtccggagag ttttgatgat tttggtgttg gataactacg
     9481 attcgttcac ctacaacctc gtgcaatacc tcggcgagtt aggcgccgag ctgaaggttg
     9541 tgcgcaacga cgccacgacc gttgccgaag tccggcggct gcggcccgca ggcattgtga
     9601 tttcacccgg cccctgcacg ccccgcgaag ccggcatttc ggtccctgtg attcgggaac
     9661 tggcgggcga aatccccatt ctaggcgtct gcctggggca ccaggccatt ggcgaagctt
     9721 tcggcgggcg cgtcgtgcgt gcgccgtatt tgatgcacgg caaaaccagc cagattcatc
     9781 acgacggcaa gacgattttc gccggcctgc cgcaggattt catcgccacc cggtatcact
     9841 cgctgatcgt cgaaaaggaa tctcttcccg gggagctcga gatttcggcc acgaccgccg
     9901 acgggctgat catgggactg cgccaccgga aattcacggt ggagggcgtt cagttccatc
     9961 ccgaatccgt gcttaccggc acgggaaagg cgattctcca gaactttctg cgcttgcggc
    10021 agccggcggc ggcctgagtt ctcccatttg gaaattcagg cgcgccgcgc agccgataag
    10081 ccgctgatcc gaatctaggc tttccggtag gcttcgtcca gcacttccac cacgtgcatc
    10141 acgcgctggc cgcgtccgaa catttcgacg cccgcccgca gttgcagcat gcagccgggg
    10201 tttgcggtga cgatggtgtc ggctccggtg gccgccgcca tctgcatctt cttttcgagc
    10261 aaacgcatcg acatctcggt gtgctgcacg ttatacacgc cggccgagcc gcagcataga
    10321 tcggaaagcg gcagctcgcg gaattgcaaa cccggaacgg cttcgagcag ccgccggggc
    10381 tggacgcgca cgcgctgccc gtgggcgaga tggcaagagt cctggtaggt cgcgataata
    10441 ttcagaggcg ccatccgccg gctcagctcg atcgagccca gaaactcgtt cacgtccttc
    10501 acttgggaag cgaacagccg cgctttttcg cgatattgtg gatcgtgctc caacaactcg
    10561 ccatactctt tcaaggtgga gccacagccg gcggcgttgg tgatcagcgc gtcatagccg
    10621 ccagcgagga aaacatcgat gttgcgccgg gcgagccggc ggcccagatc gcgcaagccg
    10681 gcgtgaacat gcagggcgcc gcagcaagtc tgcccggcgg gcacatcgac ttcgcagccg
    10741 ttctcctgta aaacccgcac cgtggcttcg ttcagccggg cgaagctgac gttcgcgagg
    10801 cagccggcca gcagggccac gcggcgccgc cgcgcgcccc gggcgggaaa ggtcttgccg
    10861 atctcatcga agaagaacgg ccgctcggcg gggggcatca gacgctccaa tttgtcgagc
    10921 cggcccagcg gtttgagcag gccggtcgag cgcaccagcc tttgcagccc gctgcgttga
    10981 tagagcgcaa cgagatttcc ggcagcttgc atcagccccg gagaaaccag aagacgtcca
    11041 tagacgaagc ggcggagcag gcgcgccagc agcggacgag gccgaacagc ctcgatgtgg
    11101 gcccgggccg cttcgaccag ctttccgtat tcgacgccgg aagggcaggc cgtttcgcaa
    11161 gcccggcacg caagacaaag ctcaatgtgc tccacgtacg aatccgaaac atccattcgg
    11221 ccgttcgcca cctgcaccat ctggtagatg cggccgcggg gcgaatccat ctccaagccc
    11281 agttcgcggt aggtcgggca cgcattcagg cacaagccac aatgcacgca gcggtccagg
    11341 tcggatggct ggggctgatc ctgcaagatg ctgagctcag agacggttat agagtcggcc
    11401 atgattgagg atgctttgag gatccaggtt atttttgatg cgggacatca aatcaagctc
    11461 cggcccggga tcggcccaga gttccagatt atctttatcg cccgtggagc aggattcaat
    11521 caccgcgtag agcccggcag cgcgggcgcg gccggtcagt tcggccggcg attcctcgca
    11581 ccgcacatag gtcactcccg tgccggcccg ggccagcgct ggccagtctc cagcggcggc
    11641 gaatgcctga tccagccgcg ccagcggtgt ggagatgcgg acgacggcgc ccgtctcgcg
    11701 cggccccgtt tccgaagagc cgttgagcgg cggcagcgca ggaaaatcgc ggaccgagcg
    11761 ccacagggac tccgcctcat cggcttccaa agagacaaat tccgcggtct cgagctgccg
    11821 ggcaagcgcc ttgagttcgc gctcatagcg cccaagaacc gcgtcgatgc cgccggcctc
    11881 gacgagcagc cagaatcctt gccccagctt cagaccgagg cgccggcaca gcgcgggatt
    11941 ggccaaatca atcactgaag gttgcagcgc gctgcgcaaa acagcgtcgc gggcttgcag
    12001 cgcccgttcg agcgattgga atgccagcac aaaggtcttt tccttgggag gccgcggaaa
    12061 caccttgaaa ttgacgctgg caatcgccgc cagagttccg aacgaaccga tcatcagctt
    12121 ggccatgtca aggccggcca cattttttac gaccatgccc cccgagcgca ccggccttcc
    12181 ttctaccgtc acgaaggtca ttccgatcac catatcgcgg gccgatccgt aaagccgccg
    12241 ccgcgggccg gaacaactgg ccgccacgac gccccccacc gtggattgct cagccagcgg
    12301 agggtcgagc ggaagcattt cattgttctc ccgaagcaat tcttggaggt tccggaagcc
    12361 tacccccgct tccacgctga tggtcagatc gcgcggctca taatcgatta cgtgattcag
    12421 ccgtgcggtc gagaccacca tgtcagcggg ctggaccgaa cctcccatcc agcgcttggt
    12481 gaaatgcccg ccgatctcga tgcttttccg cgcggcgttg caatgcgcca ggacttcttt
    12541 caattctccg atgtcggccg gctggagttt ttgcattatt cgacagtgaa ggaatggcgg
    12601 ctttcgctgg tttgctggcc aatttcatcg cgagcggcga gcacaatcgt gtattcgccc
    12661 ggcgcaatgg atttttccag gttcagattg aacgccgcgt ggacatagcg ccgcggataa
    12721 aaggacgttc ctttttcttc cgcgggctgc ggctgccgga agatcaattt gccctccgca
    12781 ttgaaaacag aaagctcata ttgcacctgc acgagattgt tgttccctgt cttgtatccc
    12841 gtaatgtcaa aagaggccca gagagcatcg ccgcggcgaa aggcgggcgc aggcaggggg
    12901 tctccatttt cttcgcgggc gaactggaaa ttccgcacca ccagcgagtc gctcggcgcc
    12961 accgcgcggc cctgcacgcg aaatggcaac tcagccgaga cttgctgtcg ggtgaagtca
    13021 tccacgacgt gaattacgaa ccgacagagg cccgattcca cccactccgg cagcgtggcg
    13081 gagaaacgca cacgcggcgt ccatttggcg tcttccggcg ccagctcggc atccaccttg
    13141 ccgctctgcg gctcaacgat aggcgtgccg gccggatcta gggtatcgat ccggtagctg
    13201 agcttcaccc gatccgaacg gtccaccgta aacccttcca cgtgaaaagc catatagagc
    13261 gtctcgccgc catgatattc ggcgtcggga gaggcgctga aaccgtcttc agcctcgagc
    13321 agcgcagcgc gcacgatgcg cagcggcttt ttttgctccg cggccgaagc cagcaaagcg
    13381 cacgcaacga gaattgcgac gctataaagc catacctgcg cgaatcgggg catcgctttg
    13441 ttccatgacc cggccattcg cgaaccggta agtttcccgc cgcgcgcccg ccaccccacg
    13501 ggcgaccatg gagggaatat ccaatttgct ttcggcccgg tcgagctcgt ccccgctcgc
    13561 gtagaggcgg gggctgcgcg gcagaaatgc ggggcagcag tcggcgaagc gctcactcga
    13621 ggtctcgtag gtgccgatgc gccgggccag atccaggatc tcccgcttgt caaagcccac
    13681 cagcgggcgg tataccggca ggcgaatggc ggcttccacc gccgccatat tctggagggt
    13741 ctgcgaagca acctgcgcga ggctgtcgcc gaccaccagt cccagggcat gacgccggtg
    13801 ggcaatccgc tccgcgatcc gcagcatcag ccggcggtaa atcagaatgc ggtattcttc
    13861 cggggcttga agcagggcct cccgctggag ctcgtggaag ggaatcagat acagccgggc
    13921 gcccaactgg tagggggcga gggtgcgcac aatctcacgc gccaccggct cgctcgcttc
    13981 gcccacccgg gcggcgacgc cgtgaaaatg aacaaagatt acgcgggcgc cgcgtttcat
    14041 catcagatag gcggccacgg cggaatcgta tccgccggag agcaggcaaa ccagcttgcc
    14101 tgccgtgttc gccgccattc cgcccggacc cggcatcttt tccgaaaaca ccagcgcgcg
    14161 gtgtccggtg aactcggcga agcaagtcgc ctcgggatga tcaagatcca cccgaatccg
    14221 gcggccggcg gcctgcgccg cgtcgagcac gtaccggccg atgcgcttct ccgcctcctg
    14281 cgaacggaaa ggcagcgtct tatcggaacg ccttacgcga atggcgaagg tatcgaacga
    14341 ttgtgccgcc agttgcttcc aggctgcttg ctccacttgg gcgaggtcgc gctccacttc
    14401 ctccgctacg gaataatgcg ccacgccgaa aatgcgcggg atgcgccgcg ccgcgccgcg
    14461 aagcgcttct tcggactcca aatgcagcag cacgcggcca tcttcatgga cgatgcgcag
    14521 cgggccgagg ccgtccaaat gagaccggat cgattcggtg aatttgtgca gaaaaaaatt
    14581 gcggttgcgg cctttcagcc acatctcgtg atagtgaagg accagcaccg gcacgctttc
    14641 attatatcgt tccagacgcg cctgtatgcg cgcgcctaca atggcggcga cggctggcgg
    14701 cgctgcgggt gcaccctcgg cgcgagccta tctatggtag gatgcggagg caaggggccg
    14761 gcttcaggca tgccatccat tctggtcatt gatgacgacg agaccgtgcg cgacaccatc
    14821 ggcatcatgc tcgagcagga aggattccgc gctcaactgg cggctgacgg ccgcgagggg
    14881 ttcgaaaaag cgctggttca gaagccggat ttgatcctgg tggacctgcg gctgccgggc
    14941 atgaacggca tggaaatttg ccgccagatc cgcgcggccc agctcagcac cccgatcatc
    15001 gtgctcagcg cggtgggcga cgaaatcgac aaagtgctcc tgctcgaaat cggcgccgac
    15061 gattatgtca caaagccgtt cggctcgcgg gaattgctgg cgcggatccg cgccgtgctg
    15121 cgccgcgctt caccggaagg gaaaaaaatt ctgcggttct ccgacgtaga ggtcgatctg
    15181 gagcgccggg tggtgatgcg ccgcagcgag cctgtcaagc tcaccccctc ggagtacaat
    15241 ctgctggtat attttctcca gaatcccgat cgcccgctta cccgcgacat gattctgaac
    15301 tccgtgtggg gctacgatac ctttcccaac acgcgcacgg tagacattca cgtggtgaaa
    15361 ttgcgccaga aactcgagcc agaccccaac attccgcggc attttacgac ggttcacggc
    15421 gtgggctacc ggtttctgcc gtgagcccgg gcggagatgt tacaccgatt ttacacggtg
    15481 cggcagccac ggcggcggcc ctatgcaaac aatcagctaa cgcctcgtcc ggaggcgcag
    15541 ggagagcgag ttctttcgcg cgcccggcgg gggcgctccc ctaaaactcg cccgcagccg
    15601 cctggcggcg caagactggc ggccgtgatt atgaccagga atatcttaat cgtcgaagat
    15661 gcggagacga ccgccaccac cctggagatc gccttgctga atcttccgaa caccaacgtt
    15721 gtgcgcgcgc ccgacgggct gcgggcgttg gaatatctgg cgccggagag cgggcatacc
    15781 atagacgcgc tggttacaga tttggagatg ccatttttgg acggctttga gctcatccgg
    15841 cgggtgcgcg agcaaaagcg gtttgccatg ctgccgattg tggtgatcag cggccggact
    15901 gacgcccaga ccctggaaaa tgtcatgaag ctgggagcga acgcgtattt cgccaaaccg
    15961 tgttcggcgc tggcgataca aaagaaaatc ctggatctac tgccatgaaa atgtttcgca
    16021 tggaaaagct ggcattctcc ttggccgcgc tgctggcggt tccgggccgc gcccagacgc
    16081 caacgtccat ggacgccatc gggcagcggc tggaacggct cgagcagcag aacgcggagc
    16141 tgctcagcga agttcgcctg ctgcggcagg agttggaaag cctcaaggca agcggcggag
    16201 cggcgcacga gagcgcgcgc gctctgaacc ctgaagcggc cgccccggaa agcgagcggc
    16261 tcgaacgttt ggaagaacgg gtagatcttc aggaaggccg gctgaacgag caggacgccg
    16321 tgaaagtgga gagcacgcag cgcgtgccgg tacgcctcac gggcatggcc ctgttcaatg
    16381 ccttcaagga caccagccac ggcggcggcg ccgacaatcc tgtggtcgct gcgccggcgc
    16441 ccggcccgat caatgccggc gccacgctgc gccagagcgt aatcgggctg gaattcgacg
    16501 ggccggaagc catcgccggc gcaaagattc gagggtcgtt tctgatggat cttttcgctg
    16561 gaaccagcgg cattttgggc agccaggcgc ggctgcgcac tgcttcgata gaagcgcgct
    16621 gggaaacccg aggctttctg gtgggccagg agaagccgat tttttcgccg cgcgagccaa
    16681 actctctggc ccaagtgggc acttcccccc tcacggcggc aggcaacctc tggctatggc
    16741 ggccgcagat ccgcttcgaa cagcgggtcg aactgggacg gaagaccgat ctgacggcgc
    16801 gcctcggcgt cttcgagacg ggcgagaacg gcgccgccct cgacccgcaa ttcgccccta
    16861 cgctcgagca gagacgcccg gcgctgcaag gccgcttcca gcttgcacac cgcttcgacg
    16921 agaatcgccg catcgagatc gcccccgggt ttcacgtcag cacgacgcac gtggcggcct
    16981 cttcggtccc atccaatgtc gtctcggttg actggttcgt gaatcccata cgccggcttg
    17041 aatttacag
//
LOCUS       DASREK010000020         4969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1662001, whole genome shotgun sequence.
ACCESSION   DASREK010000020 DASREK010000000
VERSION     DASREK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662001"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFA54_01565"
     CDS             complement(<1..241)
                     /locus_tag="VFA54_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZS49520.1"
                     /translation="MPIDETKLNQFMQQAVSELGATFNAALVVIGEKLGLYKAMAGAG
                     PLSSAELAKRTGTAERYVREWLAAQAAGGYVLYDPA"
     gene            1032..4601
                     /locus_tag="VFA54_01570"
     CDS             1032..4601
                     /locus_tag="VFA54_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49521.1"
                     /translation="MARPISIVFVMCLVFARQPLSAQLGTATISGNVTDSTGAAIVGA
                     SVTAVNAVTGFRRQTVSNTLGQYNLPGLAPGQYDLAFEFQGFKRMEQRGLTLQVDQNA
                     RIDVTLELGQVSESVEIVGQAPLVESQSATMGAVVDTQKILALPLNGRNFAQLALLVP
                     GVNTGAPGAGGAEGFSASGLRANENAFQIDGTSNSDSFQNRITVRPNIDAIQEFKIQT
                     NNYSAEFGKGGGAQVNVITRSGTREFHGGAWEFIRNNAFQARRFFDTNRRAFPCDAAD
                     PNVATRPACAPPFKQNQFGFNLGGPMFLIPRRGGDRKTFFFTTYEGFRQTRGNATLAT
                     VPSIAQRNGDFSQNLLSGNTVPDALGRTWRRGQIFDPRSSTAVTDASGRTRFVRDPFI
                     NNQIPLSRFDPVAARMLANTSFIPLPNTSGSTGGNGNPSNNFQDGRSTTSSFDQFSAR
                     IDHQFSPNDTIFGRFSFQDSNGYAPNTFPGFGQQDNQRQINVTSSYTKVLSPTMVNEF
                     RFGYQGWFQLTAAEDYIHGLDYMTEFNIRGLEYAPKAGLIGSPSFSVTGFAGWGNGNG
                     PNKARNHTFQPIEMLSFNKGRHFMKAGGELRAVMIDQTRPDTSRGSFNFDTAGWTGID
                     GVGNTGNELAAFELGLARQKARVVSNFRTEYRLREWGAFFQDDFKVTRSLTLNIGLRY
                     MYFTPPYTTDNNYGSFVYPVVCPSYAVCGANFVNFLTQPSPYVPYHGIAGKDLPRSLT
                     NTEKNDLGPRFGFAWQPLGKANTVIRGGYGIFYDTVPVQVYGDSLLNYPRVIEQQNNL
                     GLSQNGLPPVEGFIGFLVQNPGLGPGPIAQFEPGPNGFPPDFHNAYVQTWNFGVQRQL
                     PGSMVLEVAYVGTKTTRLERQEQSNTAEPLGFRATVPDLSNNPNIPDNIGSGGRNQFR
                     RLVSFTKENGIIVPLGNVFLETSLGFANYNAGTLRLEKRQSYGLTFLTTYSFSKAFSD
                     APGFGSGATNSTGGRMQNVLDRKAEKGLAELDHRHRFTAGFVYELPFGKGRAFFSGAK
                     GAMDRILGGWAVDGIFTLQSGYPMTIVRAGDPGSVGTNSALRPDLVCNPNLPRGKQTV
                     EKFFNTACYVAPESLIPGDVRYGTAGRSTAIGPGLIGTDFSARKNTAITERVRTEFRA
                     EFFNAANHPNFSTPNRNQGDGNFGKVTDTADPRIIQFGLKLIF"
     gene            4775..>4969
                     /locus_tag="VFA54_01575"
     CDS             4775..>4969
                     /locus_tag="VFA54_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49522.1"
                     /translation="MFFLSFALHAQTSPAPPSMVAGIPVNYEEALAGTYQLPDPLRLA
                     DGKPVRDAKTWFEKRRPEIVR"
BASE COUNT         1019 a         1493 c         1411 g         1046 t
ORIGIN      
        1 cagccgggtc gtagagcaca tatcccccgg cggcttgcgc cgccagccat tcccgcacgt
       61 agcgctcggc tgtgccagtg cgcttggcaa gctccgccga actgagcggg ccggccccag
      121 ccatagcctt gtacaatccg agcttttccc ctatcaccac cagcgcggcg ttgaaagtcg
      181 cgcccagctc gctgacagcc tgttgcatga actgattcag tttggtttcg tcgataggca
      241 ttgactctcc tttttctggc gaaaaaaatc cacagagctt ttctatcgcg gacgtggcga
      301 agcgccttac tgcgcaatca tacaatggcc ttcggcggcc agcaagcggc ctgcttgtgc
      361 gcatgagtct ggggattacc tttttcgacg tctccggcgt gcctgttttc gcgggttgtc
      421 agacacgata gcgtcattcc agaggggaac gagaaagctc gtttcgcgcg aggtccacca
      481 ccgttgttct tctgagcgca acaagcaatc tgcggttcta gtttgtccgg gaagcgtcaa
      541 agcggatcgc gcgaatcgcc tcggactcag acgataggac agtcccgcta aagatgcgga
      601 acgcctccgc aacagagaaa cgcgctgcgt cccgacggcg agcatccggc gcctctcgcc
      661 gcgtctctgc cgcgttgccg cccggcgtga ttccgattca gcgttcctct ccagaggatg
      721 cgcactgtgc gttcccgtat cggcggaccc tttgccgcgg cagatctttc gctgctccgg
      781 cggccggggg agcggcggcg gaagtttccg gaaagtttta caaattttcc ctcaaaaagt
      841 ctcacaaacc cgctgtggcc ggtcaacaga gcgcgtttct aacgcctcct gatgtttccc
      901 gcgccgtttc tgatggaaag cgcggccttg cttttgcaga tctaaaaatc gagttttttg
      961 caaactccta ttggccagga aaggaaccag cgctagaatc tcgtccaaca atttcagtaa
     1021 cgggagggag tatggcccgg ccaatttcta ttgtgtttgt gatgtgcctg gtgtttgcac
     1081 ggcaacctct cagcgcccag ctgggcactg ctacgatttc cggcaacgta acggactcca
     1141 ccggagcggc gattgtcgga gcttcggtga ctgccgtcaa cgcggtcacc ggattccgcc
     1201 ggcagaccgt atccaacact ctcggccaat ataatctgcc tggccttgca ccgggccaat
     1261 atgatctcgc cttcgagttc cagggcttca agcgaatgga gcaaaggggt ctgaccctgc
     1321 aagtggacca gaatgctcgc atcgacgtaa ctttggaact ggggcaggtg tcggaaagcg
     1381 tggagattgt cggccaggct cctttggtcg aaagccagag cgcgacgatg ggagcggtcg
     1441 tggacacgca gaagatttta gcccttccgc tgaacggccg taacttcgcc cagctcgctt
     1501 tgctggtccc gggagtgaat acgggcgcgc cgggcgccgg cggcgccgag ggcttttccg
     1561 ccagcggcct gagggccaac gaaaatgcat ttcaaatcga cggcacctcc aattcagatt
     1621 catttcagaa ccgcatcacg gtccggccga acatcgacgc aattcaggag ttcaagatcc
     1681 aaaccaataa ttattcggcc gagttcggca aaggcggcgg cgcccaggtg aatgtgatta
     1741 ccagatccgg cacgcgagaa ttccatggcg gagcgtggga attcattcgg aataacgcgt
     1801 tccaggcgcg ccgcttcttc gatacgaatc gcagggcgtt cccctgtgac gcagccgacc
     1861 ccaacgtagc aacccgtccc gcctgcgcgc ccccattcaa acagaaccag ttcgggttta
     1921 acctgggcgg gccgatgttc ctgataccga ggcgcggcgg agaccgcaag acgtttttct
     1981 tcacaaccta cgaaggcttc cgccaaacgc ggggcaacgc caccttggcc acggtgccca
     2041 gcatcgccca gcgcaacggc gatttcagcc agaatttgct cagcggcaat accgtgccgg
     2101 acgcgctcgg gagaacctgg agaagagggc agatcttcga tcccagaagt tccacggcgg
     2161 tgaccgacgc ctcaggaagg acgcgctttg tacgggatcc gttcatcaac aatcagattc
     2221 cgctgagccg ctttgatcct gtcgccgccc ggatgctggc taataccagc tttatacctc
     2281 ttcctaatac atcaggctcc acaggtggca acggcaatcc gagcaataat tttcaggacg
     2341 gccggtctac cacttcgagc ttcgatcaat tcagcgcgcg gatcgaccat cagttttctc
     2401 ccaatgatac gatcttcggc cggttcagtt ttcaggattc caacggctac gcgcccaaca
     2461 cgtttccggg cttcggccaa caagacaatc agcggcagat caacgtcacg tccagctata
     2521 ccaaagttct cagcccgacc atggtcaacg agttccgctt cggctaccag ggctggtttc
     2581 agctaacggc tgccgaggat tacatccacg ggctggacta tatgacggaa ttcaacattc
     2641 gcggcctcga atatgccccc aaagccggcc tgatcggctc acctagcttc tcggttaccg
     2701 gctttgccgg ttggggaaat ggaaatggtc cgaataaagc ccggaaccac acgttccaac
     2761 ccatcgaaat gctgtccttc aataaaggcc gtcattttat gaaggccggc ggtgagctgc
     2821 gcgcggtgat gatcgaccag acgcggccag atacctcgcg cggcagtttt aatttcgaca
     2881 ccgcgggatg gaccggcatc gacggcgtgg gcaacaccgg aaatgaactc gccgcattcg
     2941 agttgggctt ggctcgccag aaggcccgag tcgtgagcaa cttccgcacc gagtaccgcc
     3001 tccgcgagtg gggagccttt tttcaggatg attttaaggt aacgcggagc ctcacgctga
     3061 atatcgggtt gcggtatatg tacttcacgc cgccctatac gacggataat aactacggct
     3121 cgtttgtgta tcccgtcgtc tgtccgagtt atgccgtgtg cggcgccaac ttcgtcaact
     3181 ttctcacgca gccgagcccc tacgtgccat atcatgggat cgccggcaag gatctgccgc
     3241 gctctcttac caacaccgag aagaacgacc tgggaccccg cttcggcttc gcgtggcagc
     3301 ccttggggaa ggccaacacc gtgatccggg gaggctacgg cattttttac gacaccgtac
     3361 cggtgcaggt atatggagat tcgctgctga actacccgcg agtcattgaa cagcagaaca
     3421 acctcggcct ttctcagaat gggcttcctc ccgtcgaagg gtttatcggc tttttagtgc
     3481 aaaatcccgg cttgggaccc gggccgatcg cacagtttga gccagggcca aatggatttc
     3541 cccctgattt ccacaacgct tacgttcaga cctggaattt tggcgtccag cgccaactgc
     3601 cgggcagcat ggtgctcgaa gtggcttacg tcggcaccaa gacgacgcgg ctggagagac
     3661 aagagcaaag caacacagcc gagccgctgg gttttcgcgc cactgttccg gacctctcca
     3721 acaatccaaa tatcccggac aacatcggct ccggcgggcg caaccagttc cgccgtctgg
     3781 tctcgttcac caaggagaac ggaatcatcg tgcctttggg caacgtgttt ctcgaaacca
     3841 gccttggttt tgccaattac aacgcaggga ctctgcggct cgagaaaagg cagtcctatg
     3901 ggctgacgtt tctgacgact tactcgtttt ccaaggcgtt ctctgatgca cccgggttcg
     3961 gctccggcgc caccaacagc accggcggcc ggatgcaaaa tgttctcgac aggaaagcgg
     4021 aaaagggcct ggcggaactg gatcaccggc accggtttac tgccggcttt gtctatgagt
     4081 tgccttttgg gaaaggccgg gcgttttttt ctggagccaa aggggccatg gatcgcattc
     4141 tcggcggctg ggcggtggat gggattttca cgctgcaatc cggctacccg atgacgatcg
     4201 tgcgggcggg tgacccgggt agcgtcggca cgaatagcgc tttgcgtccg gatctggtgt
     4261 gcaatcccaa tctgccgcgc ggcaagcaaa ccgtggagaa attcttcaat accgcgtgct
     4321 acgtcgcccc cgagagcctg attcccggcg acgtccgcta tggcaccgct ggccgttcca
     4381 ccgccatcgg gccgggtctt atcggcacgg atttttcggc gcgcaaaaat acggcgatta
     4441 ccgagcgggt gcgaacggag tttcgcgcgg aattcttcaa tgcggcgaat cacccgaatt
     4501 tctccactcc aaaccgaaac cagggcgacg gcaattttgg aaaagtcacc gacaccgcag
     4561 atccgcgaat catccagttc gggctgaagc tgatattttg agccgggtcc gcccggcgcg
     4621 gtttgagccg gcgccgcctg cggcgcaagc acggttccac tgcctatttg ggtggcgttt
     4681 ctgctcaaaa aaggcacggc cgttcggcgg tcaatgggat taagatgttt gcatgctcct
     4741 gagatgctgg tgcgccattg ggtcgcttgg aataatgttc tttttgagtt ttgcgctaca
     4801 tgcccagacc tccccggcgc ctccctcgat ggtcgcaggc attcccgtta actacgagga
     4861 ggcgctcgca gggacctacc agctccccga ccctttgcgg ctcgctgatg gcaagccggt
     4921 gcgcgacgct aaaacctggt tcgagaaacg ccggccggaa attgtacgg
//
LOCUS       DASREK010000021         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_839714, whole genome shotgun sequence.
ACCESSION   DASREK010000021 DASREK010000000
VERSION     DASREK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839714"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFA54_01580"
     CDS             <1..262
                     /locus_tag="VFA54_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZS49523.1"
                     /translation="GAGFIPETLDLTVVDRVELVSNEEAIEYARRLAREEGLLSGISC
                     GAAAAVACRLARQAEFKGKMIVAILPDAGERYLSSVLFEGIV"
     gene            complement(364..732)
                     /locus_tag="VFA54_01585"
     CDS             complement(364..732)
                     /locus_tag="VFA54_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZS49524.1"
                     /translation="MAHSPAFLRLVDDAKTRIREITVEQVRRSQQEREPFYLVDCRED
                     SEWNAAHAAGSIHLGKGVIERDIESAIPDKDAKIIIYCGGGFRSALAADAVQKMGYKN
                     VFSLAGGWRAWNAAGMPTEK"
     gene            complement(896..2149)
                     /gene="murA"
                     /locus_tag="VFA54_01590"
     CDS             complement(896..2149)
                     /gene="murA"
                     /locus_tag="VFA54_01590"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZS49525.1"
                     /translation="MEKFLIRGGVPLTGSIPVSGSKNSALPALAAAVLTNDPVILHRV
                     PRVRDIRTMARLLADVGAEVKMEKGRYTIRTERITSPEAPYELVKTMRASSLILGPLL
                     ARTGRARVSLPGGCAIGARPINLHIAGLEKLGAVICQEHGYIEAQAPRGLRGAEIRFE
                     RITVTGTEDLMMAAALAEGETILENAAREPEVVDLAALLTKMGACIEGAGSSTIRIRG
                     TPRLRGAEHSIIPDRIEAGTFLVAGAITGGEIEVTGACAEHLTALTDKLQEAGVDVKA
                     AAGGLRVRGANKLAAVDVTTQEFPGFATDLQAQWMALSTQAHGASLITERIFENRFMH
                     ALELCRMGADITIQGNQAVVRGKTPLSGAGVIASDLRASASLVLAGLVASGETVVDRV
                     YHIDRGYEKIEEKLSAVGARIQRIE"
     gene            complement(2243..3136)
                     /gene="murQ"
                     /locus_tag="VFA54_01595"
     CDS             complement(2243..3136)
                     /gene="murQ"
                     /locus_tag="VFA54_01595"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HZS49526.1"
                     /translation="MLEHLGTEQRNPASAEIDLLSTEQILAIMNQEDKKVAAAVEQEL
                     SRVARAVDAIVGALERGGRLFYIGAGTSGRLGVLDASECPPTFGVPPEMVQGVIAGGE
                     AALSRATEANEDSPEAGENDLRSRGFNRNDVLVGITASGRTPYVIGAIRYAKSLGAST
                     IGLTCNAGAEISALVDVAIAPQVGPEILTGSTRLKAGTAQKMVLNMLSTASMIRLGYV
                     YRNLMVNLQLKNEKLLDRGRRIVAQAADCDFGRAAQALAEAGNDVRAAIVMVKRGVGA
                     QQARELLQAAHNRVHLVLDSR"
     gene            3174..>3611
                     /locus_tag="VFA54_01600"
     CDS             3174..>3611
                     /locus_tag="VFA54_01600"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZS49527.1"
                     /translation="MFRVRTVCLYLAAAVSALAQTGWRGSPILDEIVEQAINQNEIPG
                     AVLVVGHKGKVLHRKAYGSRSLEPSREPMTVDTIFDCASLTKVVATTSSIMLLVEEGK
                     VRLNDRAAQYLPGFAGGDITVRQLMTHFSGLRPDLDLNPPWSGY"
BASE COUNT          651 a         1181 c         1082 g          697 t
ORIGIN      
        1 cggcgccggc ttcatccccg aaacgttgga cctgacggtt gtagaccgcg tcgaattggt
       61 cagcaacgaa gaagccatcg agtatgcccg gcgtctggct cgcgaagaag ggctgctctc
      121 cggtatttcg tgcggggccg cagcagcggt cgcctgccgc ttggcccggc aggcggagtt
      181 caagggcaaa atgatcgtgg ccattctgcc cgacgccggc gaaagatatc tctcgagcgt
      241 cctcttcgaa ggaatcgttt aggcctgaag ccgatagcgc cggcagcgga attcggcgcg
      301 catgccgccg cagagcagcc cgcgcccgaa gcgccgctga tcttgggtga tcttccccgg
      361 cttctatttt tcggtgggca taccagccgc attccaggcc cgccatccgc cggccagaga
      421 aaagacgttc ttgtagccca tcttttgcac ggcatccgcc gccagagccg agcggaaccc
      481 gccgccgcag tagatgatga ttttggcgtc tttatcggga atggcgctct cgatgtcgcg
      541 ctcgatgact cctttgccaa ggtggatgga accggcggca tgagcggcgt tccactcgga
      601 atcctcccgg cagtctacga ggtaaaatgg ttcgcgctcc tgctggctgc ggcggacttg
      661 ctccaccgta atctcacgaa tccgtgtttt tgcgtcatca accagtctca ggaatgcagg
      721 tgagtgagcc atccaaaagt ctcccaatct gaatgtttct gctcggggcg cttccggcgg
      781 tcaacaagcc gcgtccgaga tctgccgaac gcgcagggga aaagcgcccg ctttgtggtc
      841 aagcccgccg ggcggcccca ggaaacgcgc gccgaatctc gcctaaggcc cgccgtcatt
      901 cgattcgctg gattcgcgcg ccgacggcgg atagtttctc ttcaattttc tcatagccgc
      961 gatcgatgtg atacacgcgg tccacaaccg tttccccgct cgccaccagg ccggcgagga
     1021 ccagagacgc gctggcgcgg agatcggagg cgatgacgcc cgccccgctc agcggcgtct
     1081 tccctctcac cacggcctga ttcccttgga tcgtgatgtc ggcgcccatg cggcacagtt
     1141 ccaaggcgtg cataaaacgg ttctcgaaga tgcgctcggt gatcagagac gcgccgtgcg
     1201 cctgggtgct gagggccatc cactgcgcct gcaagtcggt ggcgaaaccc ggaaactcct
     1261 gcgtggtcac atccaccgcc gcgagcttgt ttgcgccgcg cacgcgcaag ccgccggcgg
     1321 cggccttcac atccactccc gcttcctgca acttgtctgt gagcgcagtc aggtgttcgg
     1381 cgcacgctcc ggtgacctcg atctcgcctc cggtaatggc gccggccacc aggaaggtgc
     1441 cggcctcaat gcggtccggg atgatgctat gttccgcgcc tcgcagccgg ggcgttcctc
     1501 gaattcggat cgtgcttgac ccagcccctt cgatgcaggc gcccatcttg gtgagcagcg
     1561 cagccaggtc caccacttcc ggctcccggg ccgcgttctc gagtatcgtc tcgccctcgg
     1621 cgagcgcagc ggccatcatg aggtcttccg ttccggtcac ggtgattcgt tcgaaccgga
     1681 tttccgcccc ccgcaaacct cgaggagcct gcgcctcgat gtacccgtgc tcctggcaaa
     1741 tcacggcgcc cagcttttcg agaccggcaa tgtgcagatt aatggggcgc gctccgattg
     1801 cgcagccgcc gggcagcgaa acccgggcgc gcccggtgcg cgccaacaga ggccctaaga
     1861 tcaagctcga ggcgcgcatc gtcttgacca gctcgtaggg cgcttccgga ctggtgatgc
     1921 gctcggtgcg aatcgtatac cggccctttt ccatcttgac ttccgccccc acgtccgcca
     1981 gcaaccgcgc catcgtacgg atatcccgca cgcgaggcac gcggtgcagt atcaccggat
     2041 cgttggtgag aacggctgcg gccagcgcag gaagcgcgga attttttgag ccgctcaccg
     2101 gaatcgagcc ggtcaaagga acgccgccgc gaatcagaaa tttttccatg ccgtctgaaa
     2161 agcgcgacct acatttataa aaatccgcgc cggcgcgaac cggtaagagc gcgccgtatc
     2221 ggcgaacgcg ggccgcatgg gctcatcggg aatcgaggac caggtgaacc cggttgtgcg
     2281 cggcctggag cagctcgcga gcctgctggg cccccacgcc ccgtttcacc atcacgatcg
     2341 ccgcccgcac gtcattgccc gcttccgcca gggcctgcgc tgctcgtcca aaatcgcagt
     2401 ccgccgcctg agcgacgatc cggcggccgc gatcgagcag tttttcgttc ttgagctgga
     2461 gattcaccat gagattccgg tatacatagc cgagccggat catcgaggcg gtggaaagca
     2521 tattcaagac catcttttga gcggtgccgg ctttcaggcg agtcgatcca gtgaggattt
     2581 cggggcccac ctgaggcgcg atcgcaacat caaccagagc cgaaatttcc gcgcccgcgt
     2641 tgcacgtcaa gccgatcgtg gaagcgccca aggacttcgc gtagcgtatg gcgccgatca
     2701 cgtaaggcgt ccggccgctg gcggtgatgc ccacgagcac gtcgtttctg ttgaagccgc
     2761 gggaacgaag atcgttttcg ccggcctcgg ggctgtcctc gttggcttca gtggcgcggg
     2821 agagcgcggc ttcgccgccg gctatgacgc cctgaaccat ctccggggga accccaaacg
     2881 taggtgggca ctcgctggcg tccagcactc ccagcctgcc gctcgtgccg gcgccgatat
     2941 agaacaatct ccctccgcgt tccaacgcgc ccacaatggc atccacggcc cgagcgacgc
     3001 gcgatagttc ttgctcgacg gccgcggcca cttttttgtc ctcctgattc atgatggcca
     3061 ggatctgctc ggtggataaa aggtcgattt cggccgacgc cggatttcgc tgttcggtgc
     3121 caagatgctc gagcatctga aatacgaacc taattttacg ctaacatgac tgcatgtttc
     3181 gcgtacgaac cgtctgcctc tatctggccg cggcggtttc cgccctggca cagacgggct
     3241 ggcgtggctc acccattctc gatgagatcg tagagcaagc cattaatcaa aacgagatac
     3301 ctggggcggt gctggtcgtc gggcataaag gcaaggtcct tcaccgcaaa gcctacggca
     3361 gccggagcct cgagccttcc cgcgaaccca tgactgtaga tacgattttc gactgcgctt
     3421 cgctgaccaa ggtcgtcgcc acgacctcct ccattatgct gctggtggaa gaaggaaaag
     3481 tcaggctcaa cgaccgcgca gcgcaatatc tgcccggctt tgccggcggc gatatcaccg
     3541 tccggcaatt gatgactcac ttttcggggc tgcggcccga cctcgacttg aatccgccgt
     3601 ggagcggcta c
//
LOCUS       DASREK010000022         5676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1169825, whole genome shotgun sequence.
ACCESSION   DASREK010000022 DASREK010000000
VERSION     DASREK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169825"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VFA54_01605"
     CDS             <1..1401
                     /locus_tag="VFA54_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49528.1"
                     /translation="ELDPLSPMPLWGIALAVGPNYNLDINPEREKVAYRAIQEARKLA
                     PHAPENERAYVEALAQRYTGDPNADFKKLAVNYKNAMRELAKKYPDDPDAATLYAESM
                     MDLRPWNLWTSDGKPTDDTEEVIAVLQSVLRRNPKHAGANHFLIHAVEASPHPELGLG
                     SAKRLETLVPAAGHLLHMPSHIYLRIGDYAEATKSNEAAVKADRAYLSAAGMPSGIYG
                     LMYYSHNLQFLALSAGMEGNFIEAKKAADELAANVKPAVKQMPMVEPLLATPALVLAR
                     FQRWDDILNLPAPEPSLPMSRALWHYARGVALAAKGDPVNAQAERKQLAAASPPKPGG
                     ESGYKSSRGVLDIALEVLDARIASAQGDRKASIVHWRRAVAIQDALPYNEPPDWYYPV
                     RESLGAALACDGQYEEAERVFRADLDRNPRNPRSLFGLLKALEAQKKMADAQWVRQEF
                     EEAWKSADVSLRIEDL"
     gene            complement(1457..2809)
                     /locus_tag="VFA54_01610"
     CDS             complement(1457..2809)
                     /locus_tag="VFA54_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZS49529.1"
                     /translation="MSDWIFALGLAAVGLLAWLIGSARTRAIAAGAQAKAAAAEAREA
                     ELRQQLQAVREENRSLAEQAAAAEQARAAAEARAAQIEKNIAEQRDLLEQAKAKLSET
                     FRSLAAEALSGSNKDFLVLAEEKFRALKDEAAGRLAGLIQPLTDTLAAYQKETKELQE
                     KRGQELGSVGQQLRAVAETEAALRTETAKLVQALQSHRVRGRWGEITLRRTAELAGMS
                     PYCDFAEQERAETGDGHLRPDMIVRLPAGRSIVVDSKAPLDAFLEAMEALTDQDRETA
                     LQRYASQVRSHVMQLASKQYWAQFPSAPEFVVLFIPNDSFLGAAAERDRSLIETALNR
                     RVVFATPTTLFALLFAIERGWRQDQISQNAERVSQIGQELYDRMATLADHLIRVGGSL
                     GKAVESYNAAVTTIEGRILPSARRFKELGAAGKREIDVVPTLDQTVRRLQEPELETPK
                     "
     gene            complement(3174..4061)
                     /locus_tag="VFA54_01615"
     CDS             complement(3174..4061)
                     /locus_tag="VFA54_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS49530.1"
                     /translation="MSMKRVVLWAAACTTLLATSAWRAQAQQGPGAVRSYNTAKQKLM
                     AGKQIFGGTITSPDPNMYCAMANAGFDFLWIEMQHSPLTYEDVARLMWACRGSAAIPF
                     LRVPDATESDIQKATDIGVLGVIVPTVDTVEKAQAAVKWSKYPPQGRRSQGGGQHSAL
                     WGSDYRQTVNDNMMVVIMIETPTGVENAERIASVPGIDVIFAASSDLGNFSGHFMDPR
                     THRGDSLYESMVTRIHDVTLAHNIKLGGPEAWKERPGFSFFQSTSEPALIRAGSQALG
                     FTPAPQAGRAGGSRGSNGR"
     gene            complement(4360..4806)
                     /locus_tag="VFA54_01620"
     CDS             complement(4360..4806)
                     /locus_tag="VFA54_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZS49531.1"
                     /translation="MTNPEPIKHTDMRKPYRRVLPASTLKGDAVKNRSGEDLGKIDEI
                     MIDVPTGRVAYAVLSFGGFLGMGNKLFAVPWSVLTLDEDRKCFIFDVGKEKLERAPGF
                     DKDNWPDMADSRWGSQIYGYYGVDPYWDVEGSAAEIPPEGARRKTA"
     gene            5080..>5676
                     /locus_tag="VFA54_01625"
     CDS             5080..>5676
                     /locus_tag="VFA54_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49532.1"
                     /translation="MESRGLWIIALMLAATGAAPAQVAQPSTSPEPAATAEAPGRALP
                     AELSIPFGSVRGDLVISGDILMFIDQAQPGFSFAISRQNLNSVDIQNGMLVVELREPV
                     RNRAGETDHLVFRVLEGQTADPILAWSRAPAGGFPAQPQEQTFSYDAKHDHFIGSDSG
                     RLIIGPERIDYESVTDINQSRQWRYSDIKEIKRDSPYKI"
BASE COUNT         1100 a         1788 c         1641 g         1147 t
ORIGIN      
        1 gagctcgacc cgctgtctcc catgccgctg tggggcatag cgctcgcagt aggtcccaac
       61 tacaacctcg acatcaaccc ggagagagaa aaggtggcgt acagggcgat tcaggaggcc
      121 cgcaagctgg cgccacacgc ccctgagaat gagcgggcct atgttgaagc gctggcgcag
      181 cgctacaccg gcgatccaaa cgcagacttc aagaagctgg cggtgaatta caaaaatgcg
      241 atgcgcgagc tggcaaagaa atacccggac gacccagatg ccgccactct ctacgcggaa
      301 agcatgatgg atctgcggcc gtggaacctg tggacctccg acggaaagcc caccgatgat
      361 acggaagaag taatcgccgt gctccaatcc gtcctgcggc gcaaccccaa acatgccggg
      421 gccaatcact tcttgattca tgccgtcgag gcgtcgccgc accccgaact gggcctcggg
      481 agcgccaagc ggctcgaaac gctggttccc gccgcaggcc atttgctcca catgccctcg
      541 cacatttacc tgcgaattgg agattacgcg gaggcgacca aatcgaatga ggcggcggtg
      601 aaggccgatc gcgcctacct gagcgccgcg ggaatgccaa gcggcatata cgggctgatg
      661 tattacagcc ataatttgca atttcttgcg ctttccgccg gcatggaagg aaactttatc
      721 gaggccaaga aggcggccga tgaactggcc gctaacgtga aaccagccgt caaacagatg
      781 cccatggtcg agccactgct ggccaccccc gctctggttc tggcgcgatt tcaacgctgg
      841 gacgatattc tgaatctgcc cgcgccggaa ccctctctac ccatgtcgcg ggccctgtgg
      901 cactatgccc gcggggtcgc cctggcagcc aagggagacc cggtgaacgc ccaggcagag
      961 cgaaagcaac tcgcggcggc aagtccaccc aagccgggag gcgagtccgg ctacaagtct
     1021 agccgcggcg tgctcgatat cgcgctggaa gtcttggacg cgcgcatagc ttccgctcag
     1081 ggcgatcgga aggcatccat cgtgcattgg cgccgcgcgg tggcgatcca ggacgctctg
     1141 ccttacaacg aacctccgga ttggtactac cccgtgcgag agtcgctcgg cgcagcattg
     1201 gcttgcgacg gccagtacga ggaagcggag cgggtgtttc gagccgatct cgatcgcaac
     1261 ccgcgcaatc caaggtcgct tttcggtttg ctcaaagcgc tcgaagcgca aaagaaaatg
     1321 gcggacgcac aatgggttcg ccaggagttt gaagaagcgt ggaaatccgc ggacgtatcg
     1381 ctgcggatcg aagatcttta aaaccgccgt ctctctgcga gcgggatgca accctggaag
     1441 ttgagttcct cgcagctcat ttcggtgtct ccagttcagg ttcttgcagc cggcgtacgg
     1501 tctgatcgag agtcggaacc acgtcaatct cccttttgcc ggccgcgccc agttccttga
     1561 atctccgcgc cgaaggcaaa atgcggcctt cgatggtagt gacggcagca ttgtaggatt
     1621 cgaccgcctt ccctaaggaa ccaccaacgc ggatcagatg atcggcgagc gttgccatgc
     1681 ggtcataaag ctcctggccg atttgactga ctctttccgc gttctggctg atctgatcct
     1741 gccgccatcc gcgctcaatg gcaaatagca gcgcgaaaag agtcgtgggc gtggcaaata
     1801 cgacgcgcct gttgagagcg gtctcaatca gcgagcgatc ccgttccgcc gcagctccta
     1861 aaaaggagtc gttcggtata aagagaacaa cgaattccgg cgccgaagga aactgagccc
     1921 agtactgctt ggaggcaagc tgcatcacat gagaccgcac ttgcgacgcg tatctttgta
     1981 gagcagtttc tctatcctgg tccgtcaaag cctccatcgc ctccaaaaat gcgtcgagag
     2041 gcgccttcga gtctacgaca atgctgcggc ctgccggcag tcttacgatc atgtcaggac
     2101 ggagatggcc gtcgcccgtt tctgcgcgct cctgctcggc aaaatcgcag tacggcgaca
     2161 tgccggcgag ttcggccgtg cggcgcagcg tgatttcacc ccagcgccct cgaacgcgat
     2221 gggattgcag ggcctgcacc agcttggcgg tctcggtgcg cagcgctgcc tcggtctccg
     2281 ccacggcccg cagttgttgc ccgacgctgc ccaactcctg gcctcgcttt tcctgcaact
     2341 ccttggtttc cttttggtac gccgccagcg tatcggtgag cggctgaatc agcccggcta
     2401 gtctgccggc cgcttcgtcc ttcaaagctc tgaatttttc ttccgccagc accagaaaat
     2461 ctttgttgct gcccgacagg gcttctgccg caagggaacg gaatgtctcg gaaagcttgg
     2521 cctttgcctg ttcgaggaga tctcgttgct ccgcgatatt cttctcgatc tgagcggccc
     2581 gcgcctcggc ggccgcccgg gcctgctctg cggcggctgc ctgctcggcc agagatcgat
     2641 tttcttcgcg cactgcctga agctgctggc gcagttcagc ctcgcgcgcc tccgcggctg
     2701 cggccttggc ttgtgctccc gctgctatag ccctcgtgcg ggcgctgcca atcaaccagg
     2761 ccagcagccc aacggcggcg aggcccagcg caaaaatcca gtcgctcaac gccgctgctc
     2821 cttgccgccg gcaggcggcg ccgccacgcg ctcgctgcct ctgcctcccc cggctgcgcg
     2881 cgctgcggag ccccgggttc cagcgagcac cgcagatgcc cttgaaatca tcggagaaat
     2941 gtttcggaaa ccttctggga gagaattata gttttgggga gtcggcgccc ttcccggaaa
     3001 gatgggtggc acggccctgc atcgagaaac gggacgcaca ctcacctcaa cgaaacagaa
     3061 acatggcccg gcggccgcgg aatctccagc gggagggagc ctgaacaaca cacaggccgc
     3121 cgcaggttgg cgggcggcgg cctgctggcc gacagtcttg gtgaatcaag gatctagcgg
     3181 ccattgctgc cgcggctgcc gcccgcacgc ccagcctggg gagcaggggt gaacccgagc
     3241 gcctgcgatc ccgcccgaat caaggccggt tcgctcgtgc tctggaagaa gctgaagccc
     3301 gggcgttcct tccaagcctc gggcccgccg agcttgatgt tgtgcgccag cgtcacgtcg
     3361 tggatccggg tgaccatcga ttcgtacagc gaatcgcccc gatgagtcct tggatccatg
     3421 aagtgtcctg aaaagttgcc gagatcgctg ctagcggcaa aaatgacatc gatgcccgga
     3481 acagaagcga tcctttccgc attttccacc ccagtggggg tttcaatcat gatgaccacc
     3541 atcatattgt cgttcaccgt ctggcggtaa tcgctgcccc acagggcgct gtgctggccg
     3601 ccgccctggc tccgccgtcc ctgcggcgga tacttggacc atttcactgc cgcctgggcc
     3661 ttctcgaccg tatcaacggt aggaactatc acgcccagca cgccgatatc ggtcgccttc
     3721 tgaatatcgc tttccgttgc gtcgggcacg cgcagaaacg gtatcgccgc cgatccccgg
     3781 caggcccaca tcagccgggc cacatcttca taagtcagcg ggctgtgctg catttcgatc
     3841 cacaagaaat cgaaccccgc attcgccatc gcgcaataca tgttcggatc cggcgaggtt
     3901 atggttccgc cgaagatctg cttgccagcc attaatttct gttttgccgt gttgtaactt
     3961 ctgacagcgc ccggcccttg ctgtgcttgt gctctccacg cacttgtcgc cagcagcgtc
     4021 gtgcaggccg cggcccatag gacgactcgc ttcatgctca taaggctctc cttgtccaga
     4081 agcactgttg gattctctct cggcagctca tcccgggacg gattcccggg tgactgcaag
     4141 tatacaccac gccggaatac cacgccgttt tttgtgattt ggggccggcg ctgggagatt
     4201 cactgggtcg gccggcctat tgggaaagat gtaagcaaag cgaagcggga tcccagcccg
     4261 gagcgtcggt cctcccgctc gctgcgggcg cctcactgtt ccgcgcacag gctgcgctgt
     4321 gcgctttttc ggcaacccgc cccgccgcaa atcatgaagc tatgccgtct tccggcgggc
     4381 gccttccggc ggaatctccg ccgcggaacc ttcgacgtcc caataggggt ctacgccata
     4441 atagccataa atttgcgatc cccatctgga atcggccata tcgggccaat tgtccttgtc
     4501 gaatccggga gcgcgctcca gcttctcctt gcccacgtcg aatatgaagc atttccggtc
     4561 ttcgtcgaga gtaagcacgc tccacggcac ggcaaaaagc ttgttgccca ttccgagaaa
     4621 accgccgaac gagagaaccg cataggccac ccggcccgtg ggaacgtcga tcatgatctc
     4681 gtcaattttg ccgagatctt ctcccgatct gttttttacc gcatcgccct tcagagtgct
     4741 ggcgggaaga acgcggcgat aaggtttgcg catatctgta tgcttgattg gctctgggtt
     4801 tgtcactgcc gtctccttta ttcctttttt cgtcctcaat tgcttgatct aacgcaattg
     4861 cttcagccaa tattgatcag caggcgcggg gccaacgcaa gaatcctcac ctgccctcga
     4921 tggcgcgccc cccggtgcgg tccgggccgc caatccacaa accggctctc tctctgcgaa
     4981 ggtcgaccgt gaggtttcgg ccaggcgtgt cttccgccga ctgccgcgga tgctaacctg
     5041 aaagtaaaca gcaattcaat tcctttcgcg gagttagaca tggaaagtcg tgggttgtgg
     5101 attatcgccc taatgctggc ggcaacaggg gccgctccgg ctcaggtagc gcagccgtct
     5161 acaagtcccg aaccggccgc aaccgcggaa gcgcccggcc gggccttgcc ggccgagctc
     5221 tccattccgt ttggctccgt ccgtggggac ctcgtgattt cgggcgatat cttgatgttt
     5281 attgatcagg cccagcccgg attctcgttt gccatcagcc gtcagaattt aaattctgtc
     5341 gatattcaga acggcatgct cgtggtggag ctgagggagc cggttcgaaa ccgtgccggc
     5401 gaaaccgacc acctggtttt tcgcgtgttg gagggccaga cagccgatcc gatattggcc
     5461 tggtccagag cccctgccgg cgggtttcct gcgcagccgc aggaacaaac cttctcgtat
     5521 gacgccaaac acgatcattt cattggaagc gacagcggcc ggctcattat cggcccggag
     5581 cgcatcgatt atgagtcggt gaccgacatc aaccaatcgc gccagtggcg gtactctgat
     5641 atcaaggaga tcaagcggga tagtccctat aaaatt
//
LOCUS       DASREK010000023        10650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2158325, whole genome shotgun sequence.
ACCESSION   DASREK010000023 DASREK010000000
VERSION     DASREK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158325"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..434)
                     /gene="rpmE"
                     /locus_tag="VFA54_01630"
     CDS             complement(216..434)
                     /gene="rpmE"
                     /locus_tag="VFA54_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZS49533.1"
                     /translation="MKAGIHPTYNEVNVICACGHTFRTRSTHKGDIRVEICSNCHPFF
                     TGKQKLVDTAGRVERFERKYAKIRGGKG"
     gene            560..3853
                     /locus_tag="VFA54_01635"
     CDS             560..3853
                     /locus_tag="VFA54_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZS49534.1"
                     /translation="MHLRRTAILLVFYLLLPGLGLPAPAAQNGTGTYAVVLSAPPVGK
                     RLSVRPRTPLAMRMHRPLASMPEIQELTAQVEQSQAPVRSAIQKMGLPVTGSTHHLLN
                     AIFVRATPEQAEQLRALPGVRNVARMARYRLKLDAAAGLINAPAAWSALGGTSNAGAG
                     ITIGILDSGIDNTHPAFQDPSLPVPQGYPLGLTNYTNNKIIVAREYLSPSGTIDPATS
                     RPDDYTPRDRVGHGTFVAMIAAGETVAGPGGPTITGIAPKAYIGNYKIAGSTDINDGP
                     SQDAVLMAMDDAVNDGMFIINLSLGSIALFGPNDQGSACSSNPTTICDPVAEAVATAT
                     EQFGVIVTTPAGNDGDLGMVQPTLNTISSPGTAPDAIAVGATTNSRQLTSTLQAGSGA
                     PASLQPAPALFGNGPRPSGPLTATIRDVTDLGDDGTACSPLPASSLGGMMVLVIRGNC
                     DFSVKVQNAAAAGAVAVVVYNSPGSDQPIIMTGLTGAAIPAAMVGNSAGVALRGYIHS
                     TSSPTVTLDPKLVAQPTTADVVAGFSSRGPSIDLGAKPDLVAPGVNIYSATQSLDPNG
                     ALYDASRFTMLDGTSFSAPMAAGAAALVEQAVASNPNVFISDVISAVINTANPNVTDK
                     GAPARETAMGAGKLNVAAAINPEATVFPSSISFGVISSTNPLPISGTLVVANIGSATD
                     TFQLAVQQRDPDSKARVTLNGGSTTSIRLTSGQSANVTVALTGSTPSPGLYEGVITVK
                     GTTGPANLRVVYHYAVESNQPASIFALDGDGLVGTAGQPLQDLLILKLVDQFGLPVPN
                     ASVQFKVTQGSGSIVQADAATDVYGIAAASVNLGPEIGVQSFAATAGGLTVNFVDTAR
                     AQPSIGSGGVVNGASFASGQPVAPGSIISIFGVNLADSTASASKLPLSPALNHVSVSF
                     DVPSANISVPGYFFYVSPGQLNVQVPWELAGQTTAMLKVRIDDVVSSVYQLSLSDSAP
                     GIFQYSSGGQTLGVVTHADGSIVTPSNPAHANETVVVYATGVGPVDQPQASGEPAGAQ
                     PLANTKQTPTVTVAGQNASVFFSGLAPFFVGLNQINVTIPAGTPSGLQPLAITSNGVS
                     SNTVNIPIQ"
     gene            complement(4710..8033)
                     /locus_tag="VFA54_01640"
     CDS             complement(4710..8033)
                     /locus_tag="VFA54_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS49535.1"
                     /translation="MTHNKDLNRPNYRMGSVSRALCGLSFFFGMLALLAAAQPGRAQV
                     TAAVVGKVEDASGAAVSGATVSVKNLETGATRVVTTDDTGGFRVLSLPVGPYEVKAEK
                     TGFKAAVRTGINLAVGQEARVNLQLEVGEFAQQVTVLAEAPIVNTSTASASGVVGERQ
                     VKELPLNGRSFDYLITLNPGAINYNLKSPQTSTSNGNTFSVAGRRPLDNLVLLNGVEY
                     TGSSQLAVTPGGVSGDLLGIDAVREFNVLTETYPAEYGKRAGAQVTVVTQSGSNTLHG
                     SAFEFLRNSALDARNFFDQGSAPPFKRNQFGGALGGPIRKDRLFLFGNYEGFRQRLAV
                     SNVSVVPDEQARQGLLPNASGQYVPVPNLRREMLPYMSFWPAPNGPELLANGLPTGAA
                     LSYNNPRQSIREDFGTLRADYNLRSRDTFSASYTIDDGNSLIPLADPFFGSASVLRSQ
                     VASLDETHIISPQILNTFRVGFSRAAFNLDSALLASFPQNLSFVSGAGPGGIVIGGGT
                     TTTGAAAITSAGPNNAAGVWNRRNLFTYTDAVQISKGRHEISAGVWFQRLRDNEDSAS
                     RQLGQASFTSLTTFLQGTVSSFQVVPSATELGWRSLFGAWYFEDAIKLRPTLTLRAGV
                     RHEFTTGWNEVSGRAANYVTDAQGLLVTTPLVGRSAFTQNNAKRLFAPRIGLAWDPFG
                     NGKTAIRAGFGTYYTLIDALSFLLNSLPPYNGSVSFTNTSLFSIVPVVPTIPIPPSCG
                     PGVPQPCTTYAPQGVQANAKTPTVQEWNFTVEQELRPNTVLRLAYVGSFGYHNLLSVD
                     PNTIPAQICTDPAGCQAGGVASAANQSHVPQGTKYIPVGTRPNPYLGAGFFWYTEGNS
                     SYNALQVDLMRRLSQGLQFRANYTWSKSLDMNSGLTGAQAQNQAQMILDRNDLRRDWG
                     PSALNVTHQSSISAHYELPFGRGRRWLGAAGLQNKLIGGWDLNGIATLLSGFPFTPQI
                     GSNRSGDGDTRNPDRPSLNPSFSGPVVLGKQTRWFDPNAFVLPAAGTFGNLGRGVFEG
                     PALAELDLSLLKNTAVTEKLNLQFRAEFFNILNHTNLGTPNAVVFSGTSGAISGSAGL
                     ITSTATTSRQIQFGMKLIF"
     gene            complement(8362..9579)
                     /locus_tag="VFA54_01645"
     CDS             complement(8362..9579)
                     /locus_tag="VFA54_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZS49536.1"
                     /translation="MKWDAQLGRRRELLALSGLVAVAACFAIVAHRFASRGVLAGGLI
                     ASVLALAVGLYVVFRAWPNLPRSDIGHQLTRHGALYILTIFAVAAIALSSANNLLFLV
                     LACMLAALLASGLFSRLNLAELELQCLAPDNVFAGQEVPVRVLLRNLKSWMSSFSIWL
                     GMDLPGPREVVFPEIYFPVVAGGQTASTMVSVRFPRRGRYQQDRFWLRSGFPFGLLVK
                     TVRLRLPREILVYPALVDAREVENTLPYSISQWERRLTGPGQDLYRIRPYQTGDSSRV
                     VHWKASAHTGDLKVREFTLEEDRRVEILFDTGLPEGEEWRSRFERAVEQCASIVWRLH
                     EIGAGLKFQPGGLENVYDVLRYLAVVEPAAGGEHLPIEPCGLFQVIFTAKAENVPASL
                     PESGYCCYALEDL"
     gene            complement(9576..>10650)
                     /locus_tag="VFA54_01650"
     CDS             complement(9576..>10650)
                     /locus_tag="VFA54_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZS49537.1"
                     /translation="NHLSAMVGRICAMISVIIPTYLVVTMAGWGGAAEVWPAVVACGA
                     TFAAVQFGISNFVGPELTDILASLTSIGAMVLVMKLWRPAQIFRLEGDAPVNKAPRKH
                     SAGEVFYAWAPYILLVIFVLLWGYPPIKAVLEKGITKLIPMPGLHNMVMRMPPVTKAP
                     SPYPAVYTFNWLSAAGTSCFFAAIVSALILGMGPRKFASVTVSVSKQLALPMLTIASV
                     LGLAFLMNYAGMTSTLGLAFAATGSAFPFFSSLLGWMGVFLTGSDTSANALFGNLQVV
                     TANTLGINPILTAGVNSAGGVMGKMISLQSIAVAVAATGMPPSDEGKLFRFTLRHSVI
                     LVTVVGLIALAYAYLAPGLIPSP"
BASE COUNT         2196 a         3279 c         3201 g         1974 t
ORIGIN      
        1 tggaccggga accgtacgag ccggggaacg tattcctcat accttctgcg ctgccgccgt
       61 tccgttggcg cccccaagaa ccgaccgtgg cgcttcgcgc gtacctgccc tgacgacgcc
      121 gccggcgcag tctccaaggc cggccgtttt ccccccaagc ggaagcgaat cgcggcgcgt
      181 agccgatctt caatgggagc cgatcgaagg agagattagc ctttgccgcc gcggatcttg
      241 gcgtacttgc gctcgaaccg ctccacgcgt ccggcagtgt ccaccagttt ctgcttgccg
      301 gtaaagaaag gatggcagtt ggagcagatc tccacgcgga tatcgccctt gtgcgtcgag
      361 cgggttcgaa acgtatgccc gcaggcgcag ataacgttca cttcgttata cgtgggatgg
      421 atgccggcct tcatcaatgg tcctctcaac aatcacaaca tattcagtat aacacgcgtg
      481 caacgtgaag ccctgcgaaa gcgtcagatc acttggtgtc tgaccggatt gtttgcacgt
      541 tgaggagatg aaccgagcta tgcacctacg ccgcacagcg atactgctgg tgttctatct
      601 gctgctcccc ggcctgggct tgcccgcgcc cgcggcccag aatgggacgg gaacctacgc
      661 cgtggtgctg tcggcgcccc cggtcggcaa gcgcctttcc gtccggccga gaaccccctt
      721 ggcgatgcgg atgcaccgcc cgcttgccag catgcccgag attcaggagc tcacagcgca
      781 ggtcgagcaa agccaggcgc ctgtgcgcag cgccatccaa aaaatgggcc tgccggtcac
      841 tggctcgacg catcatcttc tgaatgcgat cttcgtgcgg gccactccgg aacaagccga
      901 gcagctccgg gccttgcccg gagtgcgcaa tgtggcccgc atggcgcgat accggctcaa
      961 gctggatgcg gccgccggcc tgatcaatgc gccggccgcc tggagcgctc tgggcggcac
     1021 ctccaatgcg ggcgccggca tcaccatcgg cattctggat tcaggaattg acaataccca
     1081 tccagccttt caggacccca gcctgcctgt gccgcagggt tatcccctgg gacttaccaa
     1141 ttacacgaac aacaaaatca tcgtggcccg cgaatatttg agcccctcgg gaaccatcga
     1201 tcccgccaca tcacgccccg acgactacac cccgcgcgac cgggtcggcc acggcacctt
     1261 tgtcgccatg atcgcagccg gagaaaccgt agccggtccg ggggggccga ccatcacggg
     1321 catcgcgccc aaggcttata ttggaaacta caagatcgcc ggatcgactg atatcaacga
     1381 cgggccgtcg caagacgccg tcttgatggc tatggatgac gccgtcaatg acggcatgtt
     1441 tatcatcaac ctgagcttgg ggtcgatcgc attgtttggt ccgaatgacc agggatcagc
     1501 ctgctcgagc aacccgacga cgatctgcga tccggtagcc gaagcggtgg ccaccgccac
     1561 cgagcagttc ggagtgatcg tgaccacgcc tgccggcaac gacggcgacc tgggaatggt
     1621 ccagccgacg cttaacacaa tcagctcgcc cggcacggcg ccggacgcga tcgcagtggg
     1681 cgctacgacg aattcccggc agctcacatc cacgttgcag gccggttcgg gcgctccagc
     1741 cagcctccag ccggctccgg cgttgttcgg caacgggcct cggccgagcg gcccactgac
     1801 ggcaacaatc cgcgacgtga ccgacctcgg cgacgacggc acggcctgtt ctcccctccc
     1861 ggcgagctcg ctcggaggca tgatggttct tgtaattcgt ggaaactgcg atttctcagt
     1921 caaggtgcag aatgccgctg cggccggagc cgtcgccgtc gtggtttata acagccccgg
     1981 cagcgatcag ccgatcataa tgactggcct caccggggct gcgattccgg cggcgatggt
     2041 gggcaattcg gcgggcgtcg ccctgcgcgg ctacatccac tccacatcca gcccgacggt
     2101 cacgctcgat cccaaactgg tagcgcagcc gaccacagcg gatgtagtcg ccggtttcag
     2161 ctcgcggggc cccagcattg acctgggcgc gaagcccgac ttggttgctc ccggcgtgaa
     2221 tatttattcg gccacgcaaa gcctcgaccc caacggggcg ctctacgatg caagccgctt
     2281 taccatgctc gatggaacca gtttttcggc ccccatggcg gccggagcgg cggcgctggt
     2341 cgagcaggcc gtcgccagca atcccaatgt attcatatcc gacgtcattt cggcggtgat
     2401 caacacggcg aatccgaatg tgaccgataa aggcgctccg gcccgggaaa ccgccatggg
     2461 cgccggaaaa ttgaacgtgg cggctgccat caatccggaa gccacggtct ttccttcgag
     2521 catcagtttc ggagttatca gcagtacaaa cccgcttccg atctcgggaa ctctggtggt
     2581 cgccaacatc ggttccgcca ccgacacgtt tcaactggct gtacagcagc gggatcccga
     2641 ctcgaaggcc cgcgtcactt taaacggcgg gagtaccacc agcatccggc tgacctccgg
     2701 gcaatctgcc aatgtcaccg ttgcgctgac cggttcgacg ccgtcgccag ggctttatga
     2761 aggcgtgatt acggtcaagg gaaccactgg cccggccaat cttcgagtcg tgtaccacta
     2821 tgccgtcgaa agcaaccagc cggcgagcat attcgcattg gatggcgacg gactcgtcgg
     2881 cacagccggc cagccgcttc aggatctgct aatactcaag ctggtcgacc aattcgggct
     2941 gccggtcccg aacgcgagcg tgcagttcaa agtgacccag ggcagcggga gcatcgtaca
     3001 ggctgatgcc gccacggacg tgtatggcat cgccgccgcg agcgtaaatt tggggccgga
     3061 aatcggggtg caaagttttg ccgcaaccgc cggcggtctg accgtgaact tcgtggatac
     3121 ggcgcgggca cagccgtcga tcggcagcgg cggcgtggta aacggcgcga gcttcgccag
     3181 cggacagccg gtcgctcccg ggtcgatcat ttccatcttt ggggtgaatc tcgccgattc
     3241 caccgccagc gcgtccaagc tgccgctttc gccggcgctc aaccacgtga gcgtcagttt
     3301 cgatgtgccc agcgcgaata tcagcgtgcc cggctacttt ttttacgtca gcccgggcca
     3361 gcttaatgtg caagtgcctt gggaattggc cgggcaaaca acggccatgc tgaaggtccg
     3421 gatcgatgat gtggtcagtt cggtttacca gttgtctctg agcgacagcg ctccggggat
     3481 ctttcagtat tcctccggag gccagacgct gggcgttgta acccatgccg atggcagcat
     3541 tgtgactccg tccaaccccg ctcacgcgaa tgaaacggtg gtggtctacg ccaccggcgt
     3601 ggggccggtg gatcagccgc aggccagcgg ggagccggcg ggggcgcagc ctttagcgaa
     3661 caccaaacaa acacccacgg tcacggtagc aggccagaac gcttccgttt tcttttcggg
     3721 ccttgcgccg tttttcgtcg gtctcaatca gataaacgtc accatcccgg cgggaactcc
     3781 cagcggcttg cagcctctgg cgatcacgag caacggcgtc tccagcaata cggtgaacat
     3841 tccgatccaa tgaggagcgg cgaacgggcg gtttcgaccc cttgcgaaaa ggggccggct
     3901 tggtaatcca gagtacgggc ggggctgggt aggttttacc ggattgagtg tggtatatcc
     3961 aattcatgtt aacctttcgt tcggttgtct cgcacgccgg gccaccgaaa gacctatgga
     4021 agagggcagc agggataggc ctgcgacaat gtccttttgc tccgccgcag tttcgaacgc
     4081 ccgcatagaa agccacattc aggagcgctg cccgaaaaat cctaatcgta gaccgaaacc
     4141 tgcggaggtc gcggagaagc gcgccaagga agcgaagaag attgcaaggc gggtgaacgc
     4201 ttgggccccg agcagcgagg gcagtaaggt cgcaccaact ggcccgcaaa gctttctggg
     4261 caatttggca gaggcccccg gccccatccg gaataagcag gggcgattag tcgcttactg
     4321 cgcatcggac gaggggacac accggggctc agggtctcgg catcgccaag ttgctggtga
     4381 gcatatcaaa aggcgggccc ttaaaacgca tcctttcaga aagcctcaac gcgtaaaacg
     4441 tcgaaaaggc agttcagggt tgatttctag gatctacagt gcccattcgg agggaaaagg
     4501 aaagagaaga aaggcaggcg cggaccgatt cggtttttgc agggaggcct cgttaatcca
     4561 cgttaataga ccattggcct gccccatctt tgtaccgggg atttcttatt gttgtccttg
     4621 tcccggagag gcagtcgcca tctggcccgc gaacagcgaa cggtctttga aaaaagcgca
     4681 ttcagctagg accaacgtgg agcggttaat caaaaaatca atttcatgcc gaattgaatt
     4741 tgccgggatg ttgtagccgt ggaggtgatg agtcccgccg agccgctaat cgccccgctc
     4801 gtgccggaaa acaccacagc gttgggcgta cccaagttgg tatggttcag aatgttgaaa
     4861 aattccgccc ggaactgcaa gttcagcttt tctgtgacgg cggtgttttt cagcagggac
     4921 agatcgagct ccgccaaagc cgggccttcg aacacgccgc ggcccagatt cccgaaagtg
     4981 cctgcggccg gcagcacgaa ggcattcgga tcaaaccagc gggtctgttt tccaagcacg
     5041 acgggcccgg agaacgacgg attcaggctg gggcggtcgg ggtttcgggt gtctccgtcc
     5101 ccggagcgat tggagccgat ctgcggcgtg aaggggaaac cgctcagcag cgtggcaatc
     5161 ccattcagat cccacccgcc gatcagcttg ttttgaagcc cggcagctcc gagccagcgc
     5221 ctgccgcggc cgaatggcag ttcataatgc gcggaaatgc tcgattggtg ggttacgttc
     5281 aaagcggaag gcccccaatc gcggcgcaaa tcattccggt ccaaaatcat ctgggcctga
     5341 ttctgcgcct gtgctccggt caggccggaa ttcatgtcca gacttttcga ccacgtgtag
     5401 ttcgcccgga attgcagccc ctgactcagc cgccgcatca ggtccacttg cagcgcgttg
     5461 tagctgctgt tgccctctgt gtaccaaaag aatccggcgc ccagataggg attgggccgg
     5521 gtccccacgg gaatatactt ggtaccctgc gggacgtggc tctggttcgc cgctgacgcg
     5581 acgcctccgg cctggcagcc agcgggatcg gtgcaaatct gggctggaat cgtattggga
     5641 tccacgctga ggaggttatg gtatccgaag gaccctacat aggccaggcg gagaaccgtg
     5701 ttcggacgca gctcctgttc gaccgtgaaa ttccattcct gaaccgtggg agtcttggca
     5761 ttcgcctgaa ctccctgcgg cgcatacgtc gtgcatggct ggggaactcc gggcccgcag
     5821 gaaggcggta tcgggatcgt cggaacaacc ggcacgatgg agaagagaga agtgtttgta
     5881 aatgaaacgg agccgttata gggcggcagt gagttcagca gaaagctgag cgcatcaatc
     5941 aaagtgtagt acgtgccaaa tcccgcgcgg atcgcggtct tgccgtttcc gaatggatcc
     6001 caggccaaac cgattcgcgg ggcgaaaaga cgcttcgcgt tgttctgtgt aaaggccgat
     6061 ctgcccacca ggggagtcgt caccagcaag ccttgcgcat cggtcacgta attggcggcc
     6121 cggccggaga cctcgttcca cccggtcgta aactcgtggc ggacgccggc tcgcagcgtc
     6181 agagtgggcc gcagcttgat tgcgtcctca aaataccaag cgccgaagag gcttctccag
     6241 ccgagctcgg tggcgctcgg aacgacttga aagctgctga ccgtgccttg caggaaggtg
     6301 gttaggctgg tgaagctggc ctggcccagt tgacgcgaag cgctgtcttc gttgtcgcgg
     6361 agccgctgaa accagacccc cgcgctgatc tcatgccgcc ctttgctgat ctgcaccgcg
     6421 tcggtatagg taaaaaggtt gcggcgattc cagaccccgg ccgcgttgtt agggccggcc
     6481 gaagtgatgg ccgcggcgcc tgtggtggtc gtgcctcctc cgatgacgat cccgcccggc
     6541 ccggctccgg aaacgaagga caggttctgc gggaaactgg ccagcagagc ggagtcgaga
     6601 ttgaaagcag cccgcgagaa gccgacacgg aacgtgttca gaatttgcgg ggagatgatg
     6661 tgggtctcat ctagactggc gacttggctg cgaaggacgg aggcggaacc gaaaaatgga
     6721 tctgccaggg gaatcaggct gttgccgtcg tcgatggtgt acgaggccga aaaggtatcg
     6781 cgagatcgca agttgtagtc cgcccgaagg gtgccgaaat cctcccgaat cgactgccgc
     6841 ggattgttat aggacagtgc agctccggta ggcagaccgt tggccagcaa ctctggaccg
     6901 ttgggcgcgg gccagaaaga catgtagggc agcatttccc gtctcagatt aggaaccgga
     6961 acgtattggc ccgaggcgtt gggcaacagg ccctgccgcg cctgttcgtc cggaaccacg
     7021 ctgacgttgc tgaccgccaa cctctgccgg aagccttcgt agttgccgaa caaaaacagg
     7081 cggtctttcc ggatcggccc gcccagagcg cctccgaatt gatttctctt aaacggagga
     7141 gcagatccct ggtcaaagaa atttcgcgcg tcgagcgcgc tgttcctcaa aaattcaaaa
     7201 gccgatccgt gcagcgtatt gctgccggac tgcgtcacca cagtgacctg tgcgccggct
     7261 cgttttccat attccgccgg atacgtctcg gtcaagacgt taaattcccg cacggcgtcg
     7321 atgcccaaca gatctccgct aaccccgcca ggcgttaccg cgagctggct tgacccggta
     7381 tactccactc cgttcagcaa gaccaggttg tccagcggcc gccttccggc gacggagaaa
     7441 gtgtttccat tgctggtgct ggtttgcggg ctcttcaggt tgtaattgat cgctcccggg
     7501 ttcagggtaa tcagataatc gaagctccgg ccgttcaggg gcagctcttt cacctggcgc
     7561 tccccgacga cgcccgaggc ggacgccgtg ctggtattaa caataggcgc ttcggccagc
     7621 acggtgacct gctgggcgaa ctccccaact tcgagttgca gattcacccg cgcttcctgg
     7681 cccaccgcca gattgattcc ggtcctgacc gcggctttaa aacccgtttt ctccgcctta
     7741 acctcgtagg ggccgaccgg caacgacaac acgcgaaaac cgcccgtgtc gtccgtagtc
     7801 acgacccggg tcgcccccgt ctccagattt ttcacgctga cggttgcgcc gctgacggcg
     7861 gctccggaag cgtcttccac tttcccgaca accgcggcag tgacctgagc ccgccccggc
     7921 tgcgcggcag cgagtagcgc cagcatgccg aagaagaagc tcaaaccgca tagcgccctg
     7981 cttacagatc ccattctgta gttgggccga ttcaggtcct tgttgtgggt catatcggtt
     8041 cgccgttcga aaaccgccgc cgcccctgga acggaacttg ccgcgacggt ctattttcga
     8101 agcttccttc gaacagaacc tcgcgaggtt ttagagccaa gagaatacca catacgcccc
     8161 cgcagtctct gtggcgaggc cggttccctt cgttaaatat ttgtcaaaaa aattgggcat
     8221 ggcgcggcgt tccgcctgta cgtcggaccg aatcaggaaa ggaaagcgca gcagaggcgc
     8281 ctatgcatta tggagtcttc tcgagcgcac gaaaggcggc ttctgggcag gcgcgccgca
     8341 tacttgcttc cggctaggcc cctataagtc ctccagggca tagcagcaat agccggactc
     8401 cggcagcgac gccggcacgt tttccgcctt ggccgtaaaa attacctgga aaagcccgca
     8461 cggctcgatc ggcagatgtt cccccccagc cgcgggttcc accacagcga ggtatcggag
     8521 aacgtcatag acgttttcca agccaccggg ttgaaatttg agtccagcgc cgatttcatg
     8581 cagccgccat acaatggatg cacattgttc taccgcccgc tcgaagcggg atcgccactc
     8641 ctcgccttcg ggaagccctg tatcgaaaag aatttccacg cgccggtcct cttccagcgt
     8701 gaattcccgc accttcagat cgcccgtgtg cgcggaagct ttccagtgca caacgcgcga
     8761 gctgtctccg gtttggtacg gccggatgcg gtaaaggtcc tggcccggcc ccgttaagcg
     8821 tctttcccac tggctgatgc tgtaaggcag cgtgttttcc acttcccggg catctaccag
     8881 cgccgggtag acgagaattt cccttggaag acgaagccgc accgtcttga caagcagccc
     8941 gaaggggaaa ccggaccgca gccaaaaccg atcctgctgg tatctgcccc ggcgtggaaa
     9001 gcggaccgaa accatggtcg aggctgtctg cccaccggcc accacgggga aatagatctc
     9061 cggaaacacc acttcccggg ggcccggcag atccatgccg agccagatgg aaaaggacga
     9121 catccaggac ttcaggtttc gcagcaggac ccgcaccggg acctcttgtc ccgcaaaaac
     9181 attatcagga gccaggcatt gtaactcgag ctccgccagg ttcaggcgcg agaacaggcc
     9241 ggatgcaagc agggccgcca gcatgcaggc aagcacgaga aagaggagat tgttggcaga
     9301 actcagcgca atcgcagcaa cagcgaatat ggttagaatg tagagcgcgc cgtggcgcgt
     9361 caactggtgg ccgatatcgg agcgaggcag gttgggccac gcccggaaca ccacatagag
     9421 ccctacggcc agggccaaca cggacgcaat caagcctccg gcgaggacgc cccggctggc
     9481 gaagcgatga gccacgatcg cgaagcaggc cgcaaccgcc accagcccgc tcagggccag
     9541 cagctcgcgc cggcggccaa gctgcgcgtc ccacttcatg gtgatggtat aagacccggc
     9601 gccagatagg cgtacgccag cgcgatcaga cctaccacgg tcaccagaat gacgctgtgg
     9661 cgcagcgtaa accggaacaa tttgccttca tcgctgggcg gcatgcccgt ggcggccaca
     9721 gcaacggcga tgctttgcag cgagatcatc ttacccatga cgcccccggc cgaattgact
     9781 ccggccgtga gaatcgggtt gatccccaag gtgttcgccg ttaccacctg gagatttcca
     9841 aacagcgcat tcgccgacgt gtcgctcccc gtcaggaaca cgcccatcca tcctagaaga
     9901 gagctgaaaa acgggaacgc cgatccggtg gccgcgaagg caaggccgag agtggaagtc
     9961 atgccggcgt agttcatcag gaaggctaac cccagaactg aggcgatcgt cagcattggc
    10021 aatgcaagct gcttgctgac cgatacggtg accgatgcaa atttcctggg gcccatcccg
    10081 agtatcagcg ccgaaacaat ggccgcaaaa aaacacgagg tgccggccgc gctcagccag
    10141 ttgaaggtgt acacggccgg gtaggggctg ggagccttgg tcaccggagg catccgcatt
    10201 accatgttgt gcaggcccgg catggggatc aattttgtga tacctttctc cagcacagct
    10261 ttgatcgggg gatagcccca caaaagcaca aaaatgacca gcagaatgta gggcgcccag
    10321 gcgtaaaaga cttcgcccgc gctgtgcttt cgaggggctt tgttcaccgg cgcgtcgccc
    10381 tccaggcgga agatctgggc cgggcgccac agcttcatca ccagcaccat ggcgccgata
    10441 gaggtgagcg aggcgaggat gtccgtcagt tccgggccca caaagttgga gatgccgaac
    10501 tgcaccgccg caaacgtcgc cccgcacgcc accactgccg gccacacctc tgccgctccg
    10561 ccccagccag ccattgtcac gaccaaataa gtcgggataa tcactgagat catggcgcag
    10621 attcgcccca ccatggcgct gaggtggttg
//
LOCUS       DASREK010000024        13629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1498381, whole genome shotgun sequence.
ACCESSION   DASREK010000024 DASREK010000000
VERSION     DASREK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13629
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498381"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFA54_01655"
     CDS             complement(<1..612)
                     /locus_tag="VFA54_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZS49538.1"
                     /translation="MRTILAAALLTVVLASGGPASAQTPNVNDNGVVDAAGFRQPVAP
                     GSIVSIFGTNFASGLATASTVPLSTSLGGTSVTFNGLPAPLFFVGPLQINAQLPSGLT
                     GNSANIVVTNGSGSSAPKAVAISPTAPGIFSVNQAGTGQGWVLFANSAVVAAPVGSIG
                     PSVQSRPAKPGDILTIYANGLGPVSPPVADGNNTLDGLRRTTND"
     gene            complement(664..2499)
                     /locus_tag="VFA54_01660"
     CDS             complement(664..2499)
                     /locus_tag="VFA54_01660"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS49539.1"
                     /translation="MRSSSNESEGVRHGALIAVLALVPLAILPAVFFYFDVTPKILVL
                     LLGTAAALLFGARGKGPALSAHQRWLHRVLALQAASLLLSTALSSDRALSLAGSNWRR
                     LGLIVQLALLAYTYLLSGLAPHRLLRAIAASGLAVSAYGIAQYSGWDPWLPKESYHIS
                     EGVWLIVRPPGTLGHAGYLGVYLVYVAFCGAALALTPEKPLWRRIGIAAASLSTAAVF
                     LSGTRAAILGLLIGALVLAVRARPRVTAKGAGLAALLIVLGAAFYFSPAGLLLRHRVQ
                     WHRGDPWGGGRVTLWTDTLRMSTHRLLAGFGPETFSSQFGRFQSAQLARAFPDRYYES
                     PHNIFLDALAGQGLLGLAALASMAALGFWAAWRGERKHPMEAETLGAGFMAALVASQF
                     LAFTLPTALLFYTMLAVLCSPSAPGASQAAARPPKFALAAAALLFTAAAFSLAWSDFW
                     IARTRDSLAQGKWEIAMEEYGHARRGVLFGTAADLWYSRAMAAALQKPLLKPPAAAWQ
                     QAFDAALRASQASEERANACYNLAAFYALRNDFSRTEQSLRAAIDWAPRWYKPHWMLA
                     QVLRESGRMAEAEAEAERAVDLNGGQHAEVLQTRNEIRMRASQQR"
     gene            2545..3927
                     /locus_tag="VFA54_01665"
     CDS             2545..3927
                     /locus_tag="VFA54_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZS49540.1"
                     /translation="MGYAHKQIASWLLKLALPLGLGIAFAAALFIRFYSGLLRIEDRP
                     AWPAYVAYFALSAALWPALETRFGIIYKCFDVSFAHWLWLLAQLDAVTMALVSSAAFF
                     WRAYSFSRYTVAVFWGLHFIFSASAAACARRWVRRNAARRSVWIFVVGNAPDLVELRR
                     ECLAGAAEAGCRRFPNISEAARALRAFDVPPDCCEVVVAVSANAAGDLDSLAEALEFL
                     PAPSSVAIEKPLGALLSGGVHFTRSFMVVSNAAGGSRAFDYVFSKRAIDLIVSAAGLI
                     LLAPVLAALAVVIWIRSGRPIFLAQQRVGRGGRKFLLYKFRTLPASCLAESDREWISP
                     ATDAWGRFLRFTGLDELPQLFNVLRGDMSLVGPRPERPHFVEQFRRDLPFYSTRHRLQ
                     VGITGWAQINGWRGDTSIRQRVEHDLYYLRHWSLAFDFRILWMTLADFFRRLWPRAVE
                     ARGTQDARSI"
     gene            3911..5029
                     /locus_tag="VFA54_01670"
     CDS             3911..5029
                     /locus_tag="VFA54_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49541.1"
                     /translation="MPDPFDSYQFLLHLRSRWRLPAATVAAATLVSLAVSLLVPKKYT
                     AHVSMVIEPPAGSDPRVATAVSPVYLESLKTYEHFAASDALFSEAADRFQLRAIEHAR
                     SVENLKRDVLKIEIPRNTKILDVAVTLGDAKTAHAVAQYIAEETIKLNRKTNRAGDDE
                     LIADAQRSVDESARKLQAAQSARLRFQRRSPGVEALKAELEQLRSLRDEVDRLAISAD
                     LTLAEQQARQKSAPADRGETESKLETASGRASELRRRASALDREIAAKQQALADHMAE
                     SDRLDARYDAAWAEHDQFEKRLRDLQSSIGFRGERLSLLDPGVVPERPSFPNIPLNLV
                     AAAALGLIVSLFYLTFEFSLRSHKAEVFRKTLRVAANT"
     gene            5026..6147
                     /locus_tag="VFA54_01675"
     CDS             5026..6147
                     /locus_tag="VFA54_01675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49542.1"
                     /translation="MKIALVHDWLTGMRGGEKVLLELVRLFPSADVFTLLWRRGSVCA
                     EIEARVRATSFLDRLPAAAKAYRYYLPLFPAAVRSLDLSGYDFVFSSSHAVAKGASIP
                     PGAVHVSYVHTPMRYLWETRGQYFQFGQGLWWKSAALQLVSPWLRRFDASSSRGVHFF
                     LANSENVRGRIRRIYGTEARVIYPPVDTEFFAPEECASRAPGPPGGDYYLVVSSLEPY
                     KRIDLAVDAFSGGCRRLLVAGAGTLHNVLRARARPPVEFLGEVSDVRLRDLYRHCRAL
                     IFPGLEDFGIAPVEAQACGRPVVCYGAGGALETVSDGRTGVHFRPQTSEALLAAVGKL
                     EKSTWDPQLIRGHSLQFSRERFRKELMSFLYDAGILAAA"
     gene            6119..7531
                     /locus_tag="VFA54_01680"
     CDS             6119..7531
                     /locus_tag="VFA54_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS49543.1"
                     /translation="MTLAYSRRRDATVSVVLGGWAGVLSLTASRPQGLWMAMLPPLLL
                     ALWWVANSATRWVLALLAAAILLPPLPLPGGDTGPHPAVLIAALGLWAGLARLSAWRM
                     RWAFLPASLLFLFFSLAISVPLAGLYSGWEIAAGSLARVALFAISAYVFFYIAWGPGR
                     EINTGRLVRVLFWAGLGSAGFACLDFYFQFPAPGRFAQQFVWLPDAVLRRAQGVFYEA
                     TTLGCFCVFLLVMIASIAALRLGRELRLPAVWLMLGAAVLLAALSFSFSRSAAASLAI
                     ALLALLWMERGRLRTTWKVITRGLGVAFCLTAAIAVVSWLFPNLLPIYFSRLWATGGY
                     LFSSPDMVLAPRLESWSFLLKALAEKPWRSIFGVGYKTLPYSGFLGRPVVADNMYLSM
                     LVETGWGGLAALLLFGIAVLRTTYRLAARGAGFRRLAGLWMFCFWCGQTVQMLSGDIL
                     TYWRLLPLYLAVLAAGVREP"
     gene            7528..8649
                     /locus_tag="VFA54_01685"
     CDS             7528..8649
                     /locus_tag="VFA54_01685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS49544.1"
                     /translation="MNILLLDQYSDLGGAQQCLLDLLPALRERGWRVRAAVPGSGPLV
                     PAMRAMGVVVDSISCGPYLLGRKSASDALRFAAGFPRLAAGIARLADGSNADLIYVNG
                     PRLLPAAAWAARRTGRPILFHCHSRLAQRYASWLAGCSLELAGAATVACCRFAAEPLA
                     RYAGSRLRIIYNAAREPPQKRSPRGPSALLRIGVVGRIAPEKGQAEFLRAARLLAPSL
                     PPCRFVICGDALFSDPGAERYRKLLYELAAGLPVDFLGWRTEVWPVLSQLDLLVTPSI
                     REPAATRVILEAYACRVPVVAFPSGGIPEVVVDGKTGFLVSPSTPAALAAKIASVLAR
                     RTKLESAALAGHVLWRERFTLERYRSEMVDAVSWAARRG"
     gene            complement(8698..9663)
                     /locus_tag="VFA54_01690"
     CDS             complement(8698..9663)
                     /locus_tag="VFA54_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS49545.1"
                     /translation="MSHCIERLPILILYPHNRCNCRCVMCDIWKIDTIQEISLSDIER
                     HRADIAALGVEWIVFSGGEPLMHSDLFRLADRLRALGIRTTILSTGLLLSKHAERIVA
                     SIDDVIVSLDGPAPVHDRIRRVAGAFGLLASGVRALQNLKPDLPVAARCTVQKQNVGH
                     LRETVRAARDLGLRSISFLAADVTSTAFNRCEPWPPGRQASVALRQEEILILEAEIEA
                     LIQDETGGFVLETPEKLRRIIRHFRAHLGLEEPAAPRCNAPWVSAVVEADGTVRPCFF
                     HSPIGNINTGGLLAVVNGQAAAEFRRTLNIAENATCRRCVCSLYR"
     gene            complement(9663..10844)
                     /locus_tag="VFA54_01695"
     CDS             complement(9663..10844)
                     /locus_tag="VFA54_01695"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS49546.1"
                     /translation="MILAGARVALGPEEAAQLDVEIRGGRIAGLRPPGGFPRKGLDLS
                     GHLILPGLINAHDHLEFALFPRLGKGPYRSAGEWARDIYHPDQSPIREHLRVPKSVRL
                     LWGGLRNLLAGVTAVCHHNPYAAVFRRNFPVRVLSNFGWAHSLDFSPDVAQRFHDTPP
                     ARPFILHLGEAINGGGRAEIFRLNEMGALDGRCVLVHAVALGRRGLQLAREKGASLIW
                     CPTSNLFTLGTTLGPAALRSGLPVALGTDSSLTAAGDLLDELRAARRARRLSDARLYR
                     MVTAEAARVLRMDQGAGGISKGGSADLAIFLDEHATPGAALFAAAACGPEMVIVQGSI
                     KLVSSRLAARLPQLRRQLLPLAVQGRGEFLVDADIPNLYQRAVRETGLEVRLGGKRVN
                     P"
     gene            complement(10841..11518)
                     /locus_tag="VFA54_01700"
     CDS             complement(10841..11518)
                     /locus_tag="VFA54_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS49547.1"
                     /translation="MNVAPLEGHRIWAATYDESPNPMLALELRTLLPRLGDLRGRKFV
                     DAACGTGRWLAEACSHEASAAGVDLSLEMLARTKRKMGRQAAIACADLLHPPLPDGWA
                     DIVVCAFSLAYVPAIGDAVRSLARLVKLGGRLYISDIHPEGRRRGWTRSFRSGPEVYK
                     IDHHFYTLEDLAKAGAKASLTLMSLLEPCFGEPEKDIFRRAGKDHLFEEAAGVPALFV
                     AEWEARP"
     gene            complement(11555..13030)
                     /locus_tag="VFA54_01705"
     CDS             complement(11555..13030)
                     /locus_tag="VFA54_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS49548.1"
                     /translation="MILFLHPRATRPKNRRFPLAILALAAVIEGKEDYVLIDGNIDPD
                     PWLSMQRAVRQGEAAGAPVELLAVSVMPGPQMAAAIPLCRAFREAYPAVPIVWGGYFA
                     SLYPDAALNAAYVDFAVRGQGEDTFLELLAALRGGRDFSKIRGLSFKDRFGLHVHNPE
                     RPLRAPDEYPWYPYYRLEAEKYIARTFLGSRTAVHQASIGCPFRCNFCGVVPVYDREK
                     MESPARTAAVLAHLQKQYGVNAIQFYDNNFYLREDHTAELADRLAPLHLKWWSEGRID
                     TVLSYSDLTLQKLRWAGAAMIFFGAESGSNEALRQMNKQITAEQTLALAKRIREFGIV
                     PEFSFILGNPRDPERDMEESIRFIRKLKRINPEAEIIVQHYIPTPQRDGMYGGIDGKI
                     QFPRTPEEWATERWYNFTIRRDPHLPWLPEHIKRRIDHFELVVNSRWPTMQDIHLNQW
                     GRALLQSLSSWRYWLGFYDFPVELKWAQKFVNLRKPRFESL"
     gene            complement(13027..>13629)
                     /locus_tag="VFA54_01710"
     CDS             complement(13027..>13629)
                     /locus_tag="VFA54_01710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS49549.1"
                     /translation="VLRPELQNILDLGAGNGWMSYRLAQRGRRPVAVDIRTDDFDGLG
                     AAQRYFSAGVEFPRVQAEFDRLPFAPGQFDLVIFNASLHYSADCRKTLAEAKRCLRPG
                     GCVVVLDSPVYKRPEHGEQMRQERRRQFEKQYGFASDSIPSREYLDEPLLGELARTAG
                     LSWRIYRPWYGWRWGLRPWKARLLGRRPPSRFWILVGTAS"
BASE COUNT         2431 a         4237 c         4308 g         2653 t
ORIGIN      
        1 atcgttggtc gtccgccgca ggccgtctag cgtgttgttt ccatcggcca ccggcggcga
       61 caccggcccg agtccgttgg catagatggt gagaatgtcg cccggtttgg ccgggcggct
      121 ttggacgctt ggtccgatgg acccgaccgg ggcggcgacc acggcggaat tggcaaacag
      181 cacccagccc tggcctgtcc cggcctgatt tacggagaaa atgcccggag cggtggggct
      241 gatggcgacc gccttgggcg cgctcgatcc cgaaccgttg gtgaccacga tgtttgcgga
      301 attgccggtc aagcccgagg gtagttgggc gttgatttgc agcggcccga caaaaaacag
      361 cggcgcgggc aggccgttga aagtcactga agttcctccg agcgaagtag aaagcggcac
      421 ggtgctggcc gtggcgagtc cggaagcgaa attagtaccg aaaattgaga cgatagatcc
      481 gggtgcgacc ggctggcgga agcctgcggc gtccaccacg ccgttgtcat ttacgttcgg
      541 cgtctgagcc gaggcgggtc ctccagaggc caggaccact gtcaataacg cagccgccag
      601 gatcgttcgc atatagctat tgtatgcggc tgcgatcgga aggatcttgt tcccggcaca
      661 attttacctt tgttgcgatg cgcgcatccg gatttcgttc ctggtctgga gcacctccgc
      721 atgttggccc ccgttcagat cgacggcgcg ttccgcttcc gcctcggcct ccgccatccg
      781 tcccgactcg cgcagcacct gagccagcat ccagtgcggc ttataccagc gcggcgccca
      841 atcgatcgcc gcgcgcaggc tctgctccgt acgcgaaaag tcgttccgca gggcgtaaaa
      901 agcggccaga ttgtaacagg cattcgcgcg ttcctcggaa gcttgcgaag cccgcagggc
      961 tgcatcgaag gcctgctgcc aggccgcggc tggcggcttt agcaatggct tctgtaaagc
     1021 tgccgccatc gcccgcgagt accacaggtc cgccgcagtt ccgaacaaga ctccccggcg
     1081 ggcatgcccg tattcctcca tcgctatttc ccatttgcct tgcgccagcg aatctctggt
     1141 gcgggcaatc caaaaatcgg accaggcgag cgaaaaggcc gctgccgtga acaatagcgc
     1201 cgcagcggct agagcgaatt ttggcggcct cgccgcagcc tggctcgccc ccggcgcgga
     1261 gggagaacaa agaacagcca acatggtgta gaaaagcaag gccgtcggca gggtgaaagc
     1321 gagaaactgg cttgcgacca gagccgccat aaatcccgct cccagcgttt ccgcctccat
     1381 cggatgcttg cgttcgccac gccacgccgc ccaaaagcct agcgccgcca tggacgcgag
     1441 cgccgccagc cccaacagac cttggcccgc caaggcgtcc agaaagatgt tgtgcggcga
     1501 ctcatagtag cgatcgggaa acgcgcgagc cagttgagcc gactggaagc ggccgaactg
     1561 tgaggagaaa gtttccggac caaagcccgc cagcagccgg tgcgtggaca tgcgtaaggt
     1621 gtcggtccaa agcgtaaccc ggccgccgcc ccaggggtcg ccgcgatgcc attgaacgcg
     1681 gtgccgcaaa agcaaaccgg ccggggagaa ataaaaagca gcgccaagaa cgatcagcag
     1741 cgctgcgaga ccggcgcctt tggccgtgac gcgcggccgg gctcgcacag ccaggacgag
     1801 cgcgccgatt agcagtccca atattgcggc tcgcgtccca ctgaggaaga cggccgcagt
     1861 cgaaaggctt gcagcggcga tgccgatccg ccgccagagg ggtttttccg gagtcaaagc
     1921 cagcgccgcg ccgcaaaacg cgacatacac taaatagact ccgagatagc cggcatgacc
     1981 gagcgttcca ggcggcctca cgatcaacca gacgccttcg ctgatgtgat agctctcctt
     2041 gggaagccag ggatcccatc cggaatactg cgcgattcca tacgctgaaa ccgccaggcc
     2101 gctggcggcg atggcgcgta gaagacgatg cggggccagg ccgctgagaa ggtatgtgta
     2161 cgccagcaga gccagttgga ctatcaggcc gagtctgcgc cagttgctgc ccgcaagaga
     2221 aagcgcgcgg tcgctggaga gcgccgtcga gaggagcagc gacgcagcct gcagcgcgag
     2281 cacccggtga agccaccgct ggtgtgcgct gagagccggc cccttgcctc gcgcgccgaa
     2341 aagcagcgcg gcggccgtcc ccaaaagtaa cactagtatc ttcggcgtca cgtcaaaata
     2401 gaaaaagact gcgggcagta tggcaagagg aaccagcgcg agcaccgcta tcagagcgcc
     2461 gtggcgcaca ccctctgatt cgtttgacga ggaccgcatg aggaccggct tgtgctttga
     2521 tagcgtatca ttcacctgag cggcatggga tacgcacaca aacaaatcgc ttcctggttg
     2581 ctgaaactcg cactgccgct gggcctggga atcgcctttg cggccgcgct gttcatacgt
     2641 ttctactccg gccttctgag gatcgaagac cggcccgcgt ggccggcgta cgtggcctat
     2701 ttcgcgcttt cggccgcgct ttggccggcc ctcgagacgc gattcggaat aatttacaag
     2761 tgtttcgacg tttcttttgc ccactggctt tggttgttgg cgcaactgga tgccgtcacg
     2821 atggcgctgg tttcttcggc ggcgtttttt tggagggcgt attcattttc acgctacact
     2881 gtcgctgttt tctggggcct gcacttcatt tttagcgcgt cggcggcggc ttgcgcccgc
     2941 aggtgggtgc ggcggaacgc ggctcgccgg tccgtatgga tatttgtagt gggtaatgcg
     3001 ccggacctgg tggaactgcg ccgcgaatgc ctcgccgggg ccgctgaggc cggctgccgc
     3061 cgttttccaa acatctctga agcagcccgt gcactgcgcg cttttgatgt tccaccggac
     3121 tgttgcgaag tcgtggtcgc ggtttctgcg aacgccgcgg gcgacctgga ttcgctggct
     3181 gaggcgcttg agtttctgcc ggcgccctcg tcggtggcga ttgaaaaacc gctgggcgcg
     3241 cttttgtccg gcggcgttca ttttacccgc tcgtttatgg tggtatccaa cgccgccggc
     3301 ggatcgaggg ccttcgatta tgttttttcc aagcgcgcta tcgacttgat agtttcggcc
     3361 gccggcctga ttctcctcgc gccggtgctg gcggcgctcg ctgttgtcat ctggatccgt
     3421 tccgggcgac cgatttttct cgcgcagcag cgcgtcggcc gcggcggccg gaagttcctg
     3481 ctttacaagt tccgaactct gcccgcttcc tgtctggcgg aaagcgaccg ggaatggatc
     3541 tcgccggcga ccgacgcctg gggccgattc ctgcgcttca ctgggctgga cgaactgccg
     3601 caattgttca atgtgcttcg gggcgatatg agcctggtag ggccccgtcc cgaacggcct
     3661 cattttgtcg agcagttccg ccgggatctg ccgttttact ccacgcggca tcgcctgcaa
     3721 gtgggcatca ctggctgggc gcagataaac ggctggcgcg gcgatacctc catacgccag
     3781 cgtgtggaac atgatttata ttatttgcgg cactggtcgc tggcgttcga tttccgtatt
     3841 ctgtggatga cgctggccga tttctttcgc cgcctgtggc cgcgggccgt agaggcaagg
     3901 ggaacccagg atgcccgatc catttgacag ctaccaattt ctgctccatc tgcgctcccg
     3961 ctggcggctg ccggccgcaa cggtcgccgc cgccaccttg gtttcgctgg ctgtgagcct
     4021 gctggttccc aaaaaataca cagcccatgt ttcgatggtg atcgaaccgc cggcgggcag
     4081 cgacccgcgc gtcgccaccg ccgtgagccc ggtttacttg gaatctctga agacctacga
     4141 gcacttcgcg gcgagcgacg ccctgttttc cgaggcggcg gaccgctttc agttgcgggc
     4201 gatcgagcac gctcgctccg ttgagaacct caagcgagat gtgctcaaaa ttgaaattcc
     4261 ccggaacacc aaaattctcg acgtcgcggt gactctcggc gacgcgaaaa cagcgcacgc
     4321 cgtggcgcaa tatatcgcgg aagaaacgat caaactgaac cggaagacca accgcgccgg
     4381 ggatgatgaa ttgatcgccg atgcgcagag gagcgtggac gagtcggccc ggaagctgca
     4441 agcagcgcag tctgcacgtc tgcgcttcca gcggcggagc ccgggcgtgg aagcattaaa
     4501 agcggaactc gaacagttgc gatcgctgcg ggatgaggtc gaccggctcg ccatttccgc
     4561 cgatcttacg ctcgccgagc agcaggcgcg gcagaaatcc gcgccggcag accgcggcga
     4621 gaccgagagt aaattggaaa ctgcgagcgg ccgcgctagc gagctaaggc gccgcgcttc
     4681 cgccctggac cgcgagattg ccgccaaaca acaggcgctc gcggatcata tggcggaatc
     4741 cgaccggctc gacgcccgct atgatgcggc ttgggccgaa cacgatcaat tcgaaaagcg
     4801 cctgcgcgac ttgcaaagtt caatcggatt ccggggcgag cgcctcagcc tgctcgatcc
     4861 gggcgtcgtg cccgagcgcc cctcgtttcc gaacatcccc ttgaatcttg ttgcagcagc
     4921 cgctttagga ctgattgttt cattgtttta tttgactttc gaattcagcc tgcgttcaca
     4981 caaggcggaa gtgttccgca aaacgctgcg cgttgccgca aacacttgaa aatcgcgctg
     5041 gtccacgact ggctgacggg aatgcgcggc ggcgaaaagg tgctgcttga gctggtccgg
     5101 ctgtttccct ccgccgacgt attcacgctg ctttggagac gtggcagtgt ttgcgccgag
     5161 attgaggccc gcgtgcgcgc aacctccttc ctggaccggc tacccgctgc ggcgaaggct
     5221 tatcgctact atctccccct gtttccggcg gcggttcgat cactcgatct ttctggatac
     5281 gatttcgttt tttccagcag tcatgccgtc gcgaagggag cttccattcc gccgggagcg
     5341 gtgcacgtga gctacgttca cacccccatg cggtatttgt gggaaaccag agggcagtac
     5401 tttcagttcg ggcaggggct atggtggaaa agcgccgcgt tgcagttggt ctcaccgtgg
     5461 ctccgccgtt tcgacgcctc cagctcccgg ggggtgcatt ttttcctcgc taattcagaa
     5521 aacgtccgag gccggatccg ccgcatttac ggaaccgagg ctcgagtgat ttacccgcca
     5581 gtcgatacag aatttttcgc tccggaggag tgcgccagcc gggcgcccgg accgccgggc
     5641 ggcgattatt atttggtggt ttcgtccctc gagccttaca agcgaatcga tttggcagta
     5701 gatgcctttt ccggcggctg ccggcggctg ctggtggctg gggcggggac cctccacaat
     5761 gttcttcgcg cgcgggcgcg gccgccggtc gagtttctcg gtgaggtcag cgatgttcgc
     5821 ctgcgggatt tatatcgtca ttgccgcgct ttgatttttc ccgggctgga ggatttcggc
     5881 attgcgccgg tcgaggctca ggcttgcggc cgccccgtcg tgtgctatgg cgccggcgga
     5941 gcgctcgaaa ccgtcagtga cggcagaacc ggcgtccatt tccggccgca gaccagcgag
     6001 gcgctgctcg ccgccgtcgg gaagctggag aagagcacct gggatccgca gctcatccgc
     6061 gggcacagtc tccagttttc acgggaacgg tttcgaaaag agctgatgag ttttttatat
     6121 gacgctggca tactcgcggc ggcgtgacgc cactgtgagc gtcgtcttgg gcggctgggc
     6181 cggagtgctg tcgctgacgg catcccgccc tcaggggctg tggatggcaa tgctgccgcc
     6241 gctgctcttg gctctctggt gggtcgcgaa cagcgccaca cgctgggttc tggctctcct
     6301 ggccgcggct atcctgctgc cgccgctgcc gctccccggc ggcgataccg gaccgcaccc
     6361 cgcggttctc atcgctgccc tgggcctgtg ggccgggctg gcgcggttga gcgcctggcg
     6421 gatgcgctgg gcttttcttc cggcctctct tttgtttctc ttcttctcgc tggccatcag
     6481 cgttcctttg gccggattgt attcgggctg ggagatcgcc gccggcagcc tggcgcgcgt
     6541 ggcgttgttc gccatttccg catacgtctt tttctatatc gcttggggcc ctggtcgcga
     6601 gatcaacacc gggcggcttg tacgcgtctt gttctgggcc ggtctgggat cggcagggtt
     6661 cgcctgtctc gatttttatt ttcaattccc agccccgggc cgcttcgctc agcaatttgt
     6721 ctggctgcct gacgcggttt tgcggcgcgc gcagggtgtg ttttacgaag cgactacgct
     6781 tggctgcttc tgtgtgtttc tgctggtaat gatcgcttct atcgccgcgc tcaggctggg
     6841 ccgcgaactg cgcctgccgg ccgtatggct catgctcgga gcggcagtct tgctcgccgc
     6901 actgagtttc tctttttcgc gctcggcagc cgccagcctg gccatagcgc ttttggcgct
     6961 tctatggatg gaacgcggcc ggctgcggac tacctggaaa gtgattacgc gcggcctggg
     7021 cgtcgcgttt tgtctgacgg ccgcgattgc tgttgtgagc tggctgtttc cgaatttact
     7081 ccccatttac ttcagccggc tgtgggctac cggcgggtat cttttttcgt ctcccgatat
     7141 ggttctcgcg ccgcgcctgg aaagctggag cttcctgctg aaggcgctcg cggaaaagcc
     7201 gtggcgcagc atttttggcg tggggtacaa aacactccct tattccggat ttctcgggcg
     7261 gcccgtggtg gcggataaca tgtacctcag catgctggtg gaaaccgggt ggggcggcct
     7321 ggcagccctc ctgcttttcg gcatcgccgt cctccgtaca acgtaccggc tcgccgctcg
     7381 aggcgccggt tttcgccggc tggccggcct ctggatgttc tgtttctggt gcggtcaaac
     7441 ggttcaaatg ctgtccggcg acatattgac ctactggcgg ctgctgccgc tgtatctggc
     7501 agtgctggcc gccggcgtcc gcgagccatg aacattctgc tcctggatca atatagcgat
     7561 ctgggcggcg cgcagcaatg cctgctcgac ctcctgccgg ctctccgcga gcggggatgg
     7621 cgtgttcgcg ccgccgtacc gggttcgggt cctctagttc cggctatgcg agcgatgggc
     7681 gtcgtggtcg attccatttc ctgcgggccg tacctgctgg ggcgaaaatc ggcgagcgat
     7741 gcgcttcgat tcgcggccgg gttcccgcgc ctcgccgccg gcattgcgcg cctggcggac
     7801 ggctccaacg ccgatctgat ttatgtgaat ggcccccgcc tgctgccggc ggcggcgtgg
     7861 gccgcacgga gaaccggccg gccgattctg ttccattgcc atagccgtct ggcgcagcgc
     7921 tacgcgtcct ggctggccgg ctgttcgctg gaactggccg gggcggccac ggtggcttgc
     7981 tgccgctttg cggctgagcc gttggcccgc tacgccggga gccggctgcg catcatctac
     8041 aatgcggctc gggagccgcc tcaaaagaga tctccgagag ggccgtctgc gctgctgcga
     8101 atcggcgtag tcggccgcat cgcgccggaa aagggccagg cggaatttct tcgcgcggcg
     8161 cggcttctcg ccccgtcgct gcccccatgc cgattcgtga tctgcggcga cgcgctgttt
     8221 tccgatcccg gagcggagcg ttaccgcaaa ctgctctatg agctggcggc aggtttgccg
     8281 gtggatttcc tgggctggcg cactgaagta tggcccgtgc tgtcacagtt ggatctgctg
     8341 gtgacgcctt caattcgcga accagcggcc acgcgtgtga tcctggaagc ctacgcgtgc
     8401 cgtgttcccg tggtggcgtt cccgtcgggc ggaattcccg aggtagttgt tgatgggaag
     8461 accggatttc ttgtaagtcc ttccacgccc gcagcgcttg cggcaaagat cgcgtcggtg
     8521 cttgcaagaa gaaccaagtt ggaaagcgcc gcgcttgccg gccatgtctt atggcgcgag
     8581 cgatttacgc tggaacgcta ccggagcgaa atggtcgatg cggtctcctg ggctgcccgc
     8641 cgcggatgag agctgcggct cccgatcgct ggaaacgcag tgcatgcgcc ggccgcttca
     8701 ccggtaaagg gaacacacac acctccggca tgtcgcattt tcggcgatat tgagcgttct
     8761 tcgaaactcg gcggccgcct gtccgtttac cacggcgagc agccctcccg tgttgatgtt
     8821 cccgatcggg gaatgaaaaa aacacggccg caccgtgcca tctgcctcta cgaccgcgga
     8881 aacccacggc gcattgcagc gcggcgcggc gggttcttcc agccccagat gggcccggaa
     8941 atgccggatg attcggcgaa gcttctcagg ggtttccagg acgaagcctc cggtctcgtc
     9001 ctgaatcaac gcttcgattt cagcctctaa aatcaaaatt tcttcctggc gcagcgccac
     9061 gctcgcctgc cttcccggag gccagggttc gcaacggttg aacgccgtcg aggtcacgtc
     9121 cgccgccaga aacgaaatag aacgcagacc cagatcgcgc gccgcacgca cagtctcccg
     9181 caaatgcccc acattctgtt tttggacagt gcagcgcgcg gccaccggca aatcgggttt
     9241 gaggttttgc aatgcccgca ctcccgacgc gagcagcccg aatgcgccgg ccacccggcg
     9301 gatgcggtcg tggacgggcg ccggtccgtc cagggaaacg attacgtcgt cgatggatgc
     9361 gacgatgcgc tccgcatgtt ttgagagcag caggcctgtg ctgagaatcg tcgtgcgaat
     9421 cccgagcgcc cgcagccggt cagccagccg gaacagatcg gagtgcatta gcggctcccc
     9481 gcccgaaaaa acaatccatt ccacgcctaa agcggcgata tcagcccggt ggcgctcgat
     9541 gtcggacaag ctgatctcct gaatcgtgtc gatcttccag atgtcgcaca tcacgcagcg
     9601 gcaattgcac cggttgtgcg gatacaaaat caggattggc aggcgctcaa tgcagtgcga
     9661 catcacgggt tgacccgctt tccgccgaga cgcacctcga ggcccgtctc ccgcacggcc
     9721 cgttggtaga gattcggaat atcagcgtca accaggaatt caccccggcc ttggacggcc
     9781 agcggcaata attggcgccg gagttgcggc agccgggcag cgagcctgga tgaaaccagc
     9841 ttgattgatc cttgcacgat taccatttcc gggccacagg cagccgcggc gaacagcgcg
     9901 gcgccgggag tcgcatgctc gtcgagaaag atggccagat cagcgctccc gcccttcgaa
     9961 attccgcctg ctccctggtc cattcgcagc actcgggcgg cctccgccgt caccatgcgg
    10021 tagagccgcg cgtcagaaag gcgccgcgca cggcgtgctg cgcggagctc atccagcagg
    10081 tcgccggctg cggtgagaga cgaatcggtt ccgagggcca cgggcaagcc gctccgcagc
    10141 gcggcagggc caagggtggt tcccagagtg aagagattgg aggtcgggca ccaaatgagc
    10201 gacgcgccct tttccctggc aagctgcaaa ccacggcggc cgagcgccac ggcgtgtacg
    10261 aggacgcaac ggccatccag cgcgcccatc tcgtttaacc ggaaaatttc ggcgcggcca
    10321 ccgccattga tcgcctcgcc aaggtgcagg atgaagggcc gggcgggagg agtgtcgtga
    10381 aaacgctgcg ccacatctgg gctgaagtca agcgaatgcg cccagccgaa gttgctgagt
    10441 acccggacgg gaaaattgcg ccggaaaacc gccgcatacg gattgtggtg gcagaccgcc
    10501 gtcactcccg ccagcagatt cctcaaaccg ccccaaagga gccggacgct cttgggtacg
    10561 cgcaggtgct cgcgaatcgg ggattggtcc gggtggtaga tgtcgcgcgc ccactcgccg
    10621 gcgctgcgat aagggccttt ccccagacgc ggaaataacg caaattcgag gtggtcatgg
    10681 gcattaataa ggccgggaag aatcaaatgc cccgacagat ccaggccctt acggggaaag
    10741 ccgccgggcg gccgcagacc ggcgatccgg ccgcctcgga tctctacatc gagctgtgcc
    10801 gcttcctcag gcccgagagc gactctggcg ccggccagta tcatggccgc gcctcccatt
    10861 cggcgacgaa gagcgccggg acgccggcgg cttcttcaaa caggtggtct ttgcccgcgc
    10921 gccgaaatat atctttttcc ggttcgccga aacacggctc gagaagactc attaaagtca
    10981 ggctcgcctt cgcgccggcc ttcgccagat cttccagcgt gtagaaatgg tgatcgattt
    11041 tgtaaacctc ggggccgctg cgaaacgagc gggtccagcc gcggcggcgg ccctccgggt
    11101 gaatatcgct gatatacagc cgtcctccaa gcttcaccag ccgggccagg ctacggaccg
    11161 cgtctccgat ggccgggacg taagcgagag aaaacgcgca tacaacgatg tcggcccagc
    11221 catccggcaa aggaggatgc aacaggtcgg cgcacgcaat tgctgcctgg cgtcccattt
    11281 tccgttttgt gcgggcgagc atttccagcg agagatctac gccagcggcc gaagcttcgt
    11341 gagaacaggc ttcggccagc cagcgtcctg tgccgcaggc ggcgtccaca aactttcgtc
    11401 cgcgaagatc gcccaaacga ggcagcagcg tgcggagctc gagcgcgagc atcgggttcg
    11461 ggctttcatc gtaagtggcc gcccagatgc ggtggccttc aagcggtgcg acattcatga
    11521 cctgaccagc ctgcctggag cgcggagcag gcgcctaaag actctcgaag cggggcttgc
    11581 gaaggtttac gaatttctgc gcccacttca gctccaccgg aaaatcataa aagcccaacc
    11641 agtatcgcca actgctcagc gattgcagaa gagcgcggcc ccactggttc agatggatgt
    11701 cctgcatagt cggccaccgg gagttgacca ccagctcgaa atgatcgatg cgccgcttga
    11761 tgtgttccgg cagccacggc agatgcggat cgcggcggat cgtgaagttg taccagcgct
    11821 cggtggccca ctcctccggc gtccgcggaa actgaatttt gccgtcgatg ccgccataca
    11881 tgccgtcgcg ctgcggagta gggatgtagt gctggacgat gatctcggct tccgggttga
    11941 ttcgcttcag cttccggata aagcggatgg attcttccat gtcgcgctcc gggtcccgag
    12001 gattgccgag aatgaaggaa aactccggga caattccgaa ttcccgaatg cgcttcgcca
    12061 gagccagtgt ttgctcggcc gtgatctgct tgttcatctg ccgcagcgcc tcgttggatc
    12121 cggattcggc tccgaaaaaa atcatcgccg cacccgccca gcgcaacttt tgcagcgtca
    12181 agtccgagta actcagcacc gtatcgatgc ggccttcgct ccaccacttc aggtggaggg
    12241 gcgccaggcg gtcggccagt tccgccgtgt gatcctcgcg caggtaaaaa ttattgtcgt
    12301 aaaactggat ggcgttgacg ccatactgct tttgcaaatg ggccaaaacc gccgccgtgc
    12361 gcgccggcga ttccattttt tcccgatcgt aaaccgggac cacgccgcag aaattacacc
    12421 ggaaggggca gccgatgctc gcctggtgta cggcggtgcg cgaacccaga aaggtacgcg
    12481 cgatatattt ttcggcctcc aggcggtaat agggatacca gggatactcg tcgggcgcgc
    12541 gcaggggccg ttccgggttg tgaacatgga ggccgaagcg gtctttgaag gagagtccgc
    12601 gaatcttcga aaaatcccgg ccgccccgca acgccgccag cagctcgagg aaagtatctt
    12661 cgccctggcc gcgaacggcg aagtcaacat aggccgcgtt cagcgccgca tccggataga
    12721 gggaagcgaa atagccgccc catacgatcg gcacggctgg atatgcctcg cgaaaggccc
    12781 ggcagagggg gattgcggcc gccatctgcg gcccgggcat gacggagacc gccagcagct
    12841 cgaccggggc gcccgccgcc tctccctgcc gcacggcgcg ctgcatcgat agccacggat
    12901 cggggtcgat gttgccgtct atcagtacat aatcttcttt gccctcgatg acggcggcaa
    12961 gggcgagaat cgcgagagga aagcgccggt tcttcggccg cgtcgcgcgc ggatggagaa
    13021 aaagaatcat gatgctgtcc ctacgagaat ccagaagcgc gaaggcggcc gccgccccag
    13081 caggcgcgct ttccagggac gcaagcccca gcgccagccg taccagggcc ggtaaatccg
    13141 ccagctcagg ccggcggtgc gggccagctc cccgagcagc ggttcatcga gatactcgcg
    13201 gctaggaatg ctgtcggaag cgaacccgta ctgcttttca aactgccgcc gccgctcctg
    13261 acgcatctgt tcgccgtgct cggggcgctt gtaaacaggc gaatcgagga cgacaacgca
    13321 tccgccgggc cgcaggcacc gcttcgcttc ggcgagcgtt ttccggcaat cggcggagta
    13381 gtgcagcgag gcattgaaaa tcaccaggtc aaactgcccg ggggcgaatg gcagccggtc
    13441 gaattccgct tgaaccctgg gaaattccac tcccgcggaa aaatagcgct gggctgcgcc
    13501 gaggccgtcg aaatcgtcgg tccgaatatc cacagccacc ggccggcgcc cgcgctgcgc
    13561 gagacgatag gacatccagc cgttgcccgc tccgagatcg agaatatttt gcaactccgg
    13621 ccggagaac
//
LOCUS       DASREK010000025        27860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_345551, whole genome shotgun sequence.
ACCESSION   DASREK010000025 DASREK010000000
VERSION     DASREK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 27860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27860
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345551"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFA54_01715"
     CDS             complement(<1..346)
                     /locus_tag="VFA54_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS49550.1"
                     /translation="MNLLVILRVALRALSRHKMRSVLTMLGIIIGVGSVITMVGVGQG
                     AKAQVQDRMAVLGTNTLQINAGSGGMGGIRGGLGSIRTLVTDDAEAILRQVPGVLRVT
                     PGASGSVQIIYGN"
     gene            complement(491..1708)
                     /locus_tag="VFA54_01720"
     CDS             complement(491..1708)
                     /locus_tag="VFA54_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS49551.1"
                     /translation="MNLLVTLRVALRALSRHKMRSALTMLGIIIGVGSVITMVGVGQG
                     ANVQVQERIATLGSNALMVGAGSGGGGHVHGGLGSIRTLVVDDMFAIQRQVSGVRAVA
                     PGATGSVQIIYGNQNWQTGVHGVTPSYFQIKETPMELGSCFTDEDVQLGNNVVILGAE
                     VRKQLFGDENPIGKTVRIKNLPFRVVGVTVAQGQSGMGFNQDDRIFMPYTTAQKKVLG
                     ITWLNYIQVQAVSREAIYMVQKQVEDLLRERHRIKPTDPDDFFVRNNVDVAQLADESS
                     QVMTLMLASVASVSLLVGGIGIMNIMLVSVTERTREIGVRMAIGATEQDVQAQFLTEA
                     MVLSLFGGLLGIIFGLTASKILSTVLEWPSRISLVSIAVSVLFSAAMGVFFGLYPARK
                     AARLDPIEALRYE"
     gene            complement(1705..2508)
                     /locus_tag="VFA54_01725"
     CDS             complement(1705..2508)
                     /locus_tag="VFA54_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS49552.1"
                     /translation="MASNNGNSPQVGPGSNGTVIHIDHVHKIYDLGEVQVHALRGISL
                     DISRQEFVAIMGASGSGKSTFMNILGCLDRPTRGSYFLEGTNVAGLTKTELARIRNRH
                     IGFVFQGFNLLARTTALENTELPGIYAGMNSEERRARAMEALRQVGLHGREHHFPSQL
                     SGGQQQRVAIARALMNRPSILLADEPTGNLDSRTSVEIMDILQQLNEREGLTIVLVTH
                     EPDIAAYAKRTVIFADGKIRKDEKVQKRLIAAEVLPQMKLVGDDEEEEA"
     gene            complement(2521..4098)
                     /locus_tag="VFA54_01730"
     CDS             complement(2521..4098)
                     /locus_tag="VFA54_01730"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS49553.1"
                     /translation="MDTPLIEQKKPTRFLTSKRILVVGVILAGATLLAAFARSRKKDP
                     VYFTSTVERGDIRSEVTATGTINAVITVQVGSQVSGIIDKLFVDFNSRVKKGQVIAQL
                     DQSIFKAQVQQAQADLENSAANVRALEANIETMKADLASAEANVEKAMAAERQAKLDY
                     DRNLDLFKQGIVAAQVRDQAQSTYDAAVASTKAAKAAAEHSRAMVDTAVSQLAQAKAQ
                     VEQKKAALELAKVNLAYTTIYAPVDGTVVARNVDVGQTVAASLQAPTLYTIAQDLTKM
                     LVYAKTDESDVGNIHVGAEATFKVDAYPRETFHGRVSQIRMNPQTIQNVVTYDTVIEF
                     DNPEQKLLPGMTAYVTIPVAEARNAIKIPNGALRFRLEEPERQALFAKYQFKDRGASR
                     AGLTAPDGEGNGGGNGGREGRRAGAGRGKRGGGGGGGDPEVASAEPERKAPVRRPAVQ
                     DIRTLYVLQPDKTLRPVVVRTGITDFTYTECAAILQGELKPGEEVVTGKELPNRGISG
                     MLGRGGIGPMGGMRGPR"
     gene            complement(4201..4584)
                     /locus_tag="VFA54_01735"
     CDS             complement(4201..4584)
                     /locus_tag="VFA54_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49554.1"
                     /translation="MVDPKVAGCKGKLFALLGLVFVSGMLAGAVTLKLVEHYGSRRPG
                     PVLTEAEKQMAVDHFSQELDLNSEQVKAIEDILDEVIMEQANLMQQFQHSRTSGHDQI
                     LHILNADQRKRFQKVLSELGAKRQD"
     gene            complement(4588..5070)
                     /locus_tag="VFA54_01740"
     CDS             complement(4588..5070)
                     /locus_tag="VFA54_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZS49555.1"
                     /translation="MHVLLRDNLEGYLSGNLDSATLGEVESHLAECSGCRQEWIVLRQ
                     SAEAIRSLRPPLAMDWDLAPGFYARVMERIDEEREIPFWAMLLDPAFGRKLVFACLML
                     LALLGTYVAAFEKPDYALQHLPEAVLAGQESSAPAPRIGPSLDRNRGVMLATLVADGD
                     "
     gene            complement(5164..5814)
                     /locus_tag="VFA54_01745"
     CDS             complement(5164..5814)
                     /locus_tag="VFA54_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS49556.1"
                     /translation="MATGVFPLPATLSEAPNKAKVEKHDSSEAALVKRCQAGDEIAFR
                     EIVERHQSKVYSIIYGIVRHRNDAEDIAQQVFTKVYFSIRGFDLRSSLLTWIYKITVN
                     ECYDYLRKQKVRRLVYESDMSEEESKRLENTQTPVNREQPIDQRLEKRDYILKLLMKV
                     PEEERMLLIEKEVEGRTVEELSAATGLNENTIKVKLFRARQRLVKAAGRLGKTWAL"
     gene            6289..8292
                     /gene="uvrB"
                     /locus_tag="VFA54_01750"
     CDS             6289..8292
                     /gene="uvrB"
                     /locus_tag="VFA54_01750"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZS49557.1"
                     /translation="MDFKLVSPYSPRGDQVTAIEQLVRGVMEGEAHQVLLGVTGSGKT
                     YTMAKVIERINRPTLVLAHNKTLAAQLYNEFKTFFPHNAVEYFVSYYDYYQPEAYMPA
                     TDTYIEKEATINDELDKLRLSATRSLFERRDCLIVASVSCIYGLGSPEAYYGMLLMLE
                     KGQKISRNQILSRLVEILYERSETDFKRGAFRVRGDTIEIYPTYDDYAYRIELWGDQV
                     ENLYQIDPLTGQVRQSYLRLPIYPKTHYVMSDDTRQKAIQSILAELAWWKAELEKEGK
                     YLEAQRLFQRTMFDVEMMREIGYCHGIENYSRHFSGRLPGEAPPTLLDYLPQDSLLFI
                     DESHQTIPQLHGMYHGDRSRKETLVKHGFRLPSALDNRPLTFEEFEHRVNQVIYVSAT
                     PGPYELTKSAGVVVEQLIRPTGLVDPQVEIRPIRGQVDDLLHEIRRAAERNERVLVTT
                     LTKRMAEDLTEYYSELGVRCRYLHSEIETLERIKILRDLRKGEFDVLIGINLLREGLD
                     LPEVSLVAILDADKEGFLRSSGSLIQTIGRAARHINGRAVLYADRITDSMAQALAETN
                     RRRAVQMQYNEANNIVPQSILKPIDMSLVQVAEGDYVTVPLEAETDLASLTPEEAGRY
                     ISELEQSMREAARKFEFEKAAQLRDRIKSLKTREIVGTMQSAG"
     gene            8304..9146
                     /locus_tag="VFA54_01755"
     CDS             8304..9146
                     /locus_tag="VFA54_01755"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HZS49558.1"
                     /translation="MLRVGLIGPGRAGQALVRLLPPSHYEIGPVLGRTFTSARRVTRQ
                     MRLGEPTESYEDFAACDLIFITVPDDAIASSAQALARAAFPFRRKVVLHASGALDSSV
                     LAPLRERGASVGSLHPLQTFGRRVLSLAGIHFAVEGDETAIRLAQSLVTHWHGKLLRI
                     KADQKIFYHAAATFASPLFTALLEAAVRLMGRAGVRPKTAIRALRPLLVTTFENYLHT
                     GKQSWTGPLARGDAVTVRRHIKALAEYDPDLACYYRAASVSALSLFSRHPELQIQLRR
                     LRIR"
     gene            9143..9487
                     /locus_tag="VFA54_01760"
     CDS             9143..9487
                     /locus_tag="VFA54_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZS49559.1"
                     /translation="MKHWLRQDALHAFLWLAASLLLAAGSLAAKKKPQAPVDINTASA
                     AELAQLPGIGPSRAAQIVRLREKNGPFRSVDELRALPRLSEKQFLELKQYTTAGSSPR
                     PKPNAANAQPQR"
     gene            9591..11276
                     /locus_tag="VFA54_01765"
     CDS             9591..11276
                     /locus_tag="VFA54_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49560.1"
                     /translation="MRRIPGINTGLGCLILLLCIALQDFPAPAQIGYPGGGYPGGYPG
                     GYPPGGYPPGGYPPGGYPGGGYPYPGGGGVGFPAPRRRGKQDQTKNNKDAEDRVNVSG
                     LLRKLDDSSIIVQAQDTRIITLKRTSQTKFQRNSKDIRPSVLKPGDHLRVEAHQDDQG
                     YLYALTVFLEKQGTNEERAAASQPVEAQVSPTGTQGDPDTPGLRGTEPAHADDPDIPV
                     FRRKTPKDSPAPPDSADSATPTAGGTPAAPAQAGIPSSDDVASTTKIESPPSLPNEDD
                     SDRPRLRRGWSRGKPLPDSDNGVQAASNRPAMKEPMPGTGAREPDETAAARSSDPVIE
                     KARAAAAEFTETLPDYVCEEQMTRFVNVSHVVNWQPVDIVSSEVVYEKGRERYRNLKV
                     NGKPSGKSMEELGGAWSTGEFGSVLVDLLSPATAAEFEFRRESSAAGQPALVYDFSVE
                     QEHSHWRIQVPSQTVIPAYKGSVWIDKKTNRVLRIEMQTRNMPREFPLDKVESAVDYE
                     YVRLGGEKLYLLPVHAESLSCQRGTSQCSRNSIDFRNYHKYTSESEITFTDPK"
     gene            complement(11312..12511)
                     /locus_tag="VFA54_01770"
     CDS             complement(11312..12511)
                     /locus_tag="VFA54_01770"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZS49561.1"
                     /translation="MRALIPLVLAAASAGAQTTVSARAQRLLDRAVVLDLHDDTTQMI
                     LDEGYNLGELHDYGQVDIPRMRRGRVSGIFLSIWTDSARYTPLQSIKRALDEIDADRR
                     EIARHPADLVLATSSDEIVAAKKQGRIAMLMGVEGGHMIDSDLAILHTYYALGARYLT
                     LTHSTPTPWAGSSGGKSEPDGLNDFGRQVVRELNRIGMMVDVSHVSDKTFYDTLAVSR
                     APVIASHSSCRALGGAARDMTDDMLRALAKNGGVVHINFYNAFLDPDFAKRSSALKDL
                     NAREEAINKEFAGDPARRAAEQRKIDAERLERLGRVPLSRLLDHFEHAVKVAGVDHVG
                     LGSDFDGANDQFPEGMEDISKIPNLVQGLMERGFSDADIEKILGGNTLRVMREVERVA
                     RRTQKAN"
     gene            complement(12517..13077)
                     /locus_tag="VFA54_01775"
     CDS             complement(12517..13077)
                     /locus_tag="VFA54_01775"
                     /inference="COORDINATES: protein motif:HMM:NF042809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZS49562.1"
                     /translation="MAGGECQCPKIDLADWRDREVTLSGHQFLSAQTPIFLHVPRRLY
                     REVEALENQIRRLGLRPAGAPLILHRDGWFWGEVLVSVDPHPRSSPEIQSFQNLFYSR
                     VVDEPGFDAALREMPRFYRDLRAAGVGRIESMYFWYLSCPRCLIEHGARQIILLARSN
                     KLLASESCPLMASAAAPTPRALPCGI"
     gene            complement(13610..17089)
                     /locus_tag="VFA54_01780"
     CDS             complement(13610..17089)
                     /locus_tag="VFA54_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49563.1"
                     /translation="MRTQFLRRFLLYSMLLTLLPAIDGPSLWADVTATILGIATDPSG
                     AALPGVQISVTNLDTNLTRMTVTDTVGQYRILALPVGRYKLEATLSGFQKFVADDIVL
                     TVNEQRRVHINLQVGSLEQQVEVTANPIQVETASTQMGQVIDDKKILSLPLNGRSFVD
                     LLGLQAGVVPVNSGASRSAAVSGQLSAGNVSVNGQRESSNAFLVNGGDVSEGRNFGTA
                     IIPNLDSVAEFRLITNSFDAEYGRFSGAVMNAITKSGSNSVHGTAFEFLRNDKLDSRG
                     FFDPQRGILKRNQFGYAVGGPAIKDKLFWFTDYQGTRQSQGASTGLIQVPTLDQRSGN
                     IGAGALNGTVNGSYWAGVLSQRLGYTVTANEPYSLPGCTSTATCVFPNGVIPARAFSP
                     IAANLLKYIPLPNAGPNTFADSSQASRIGDDKAGQRVDFLNRKTGNWYAYYYFDDSTI
                     SNPLGTDSFPGFPSSTPTRAQQGVLSNVRTFGPSAVNEARLSFTRTATHANQPGDPGV
                     SLSSLGFITGVNTLGIVPSGPPNFEAVPPISLINFTLGRTIGTTGQFNNTWHASENFS
                     KIHTTHSFKFGGEFRYLQINERNIYAPNGNFSFDGSETGSDIADFLLGAPSQYIQASF
                     QVLDSRTKYAGIYGQDSWRIRPNLTFNYGLRWEFSQPWYDTQDKIETMIPGVQSTVFP
                     TAPKGWLVPGDPGVPRTLAPTDYNNFGPRAGLAWSPNVSGGVWGKLLGGPGKTSIRAS
                     YGLYYTAIEDATLFVIVADAPYGLFWVSSVPPLFNEPFRTRSDGSSQQQRFPFILPTP
                     GDPSLKTMDWSVFLPIASSPGYAVTNRLPYASHYNFSIQRQLSSSMVLTLAYVGTQGH
                     KLIAEVESNPGNPALCLSLRGSGVKAGTPQCGPNQENGIFTRPDGSQVFGTRAPFGFN
                     FGHNDYESTLANSSYNSFQATLERRASDFTFLAAYTFGKSMDNASGFGNRVNFSNYAL
                     SRSLSQFDITHNFVFSTSYAIPFGRLFRGAPKGLTQGWNIAGVTRFATGLPVTISQSG
                     DRSLVGAPGIDVPNFVGPLTMQDPRNPGADGKPNTYFNKTAFTTGPLGGFGNANRRFF
                     HGPGFNNWDYALVKNTVLHEQMALQFRAEFFNLFNHAQFNNPQGNFSNGRFGQVSSAR
                     DPRIGQLSLKFLW"
     gene            complement(17594..19003)
                     /gene="boxB"
                     /locus_tag="VFA54_01785"
     CDS             complement(17594..19003)
                     /gene="boxB"
                     /locus_tag="VFA54_01785"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HZS49564.1"
                     /translation="MSLYDKIPNNVNLSNDKRLVRALEHWLPNYMRWWQEMGPAGFQA
                     DRVYLRTAVSVEPGGWAHFDYVRMPEYRWGIFLAPPEKDRRIGFGDHYGQPVWQEVPG
                     EYRNMLRRIIVTQGDTEPASVEQQRLLGRTTPSLYDLRNLFQVNVEEGRHLWAMVYLL
                     HSYFGREGREETEELLERRSGDADKPRILGAFNEPIHDWLAFYMFTTFTDRDGKYQLL
                     ALAESGFDPLSRTTRFMLTEEAHHMFVGQSGVDRVVKRACEIMRETGDDDVRRQGAID
                     LETVQRYINLWYSVSLDLFGGEISSNAANFFAAGLKGRYKEETFSDHLALEGSYRILE
                     AGPNGWEQKEVPLRNAMNEVLRDAYIADCESALARWNRTIAGFGIPYRLRLPDRRFHR
                     RIGVYSAGRFDPQGRPMTAEQWESRRHEFLPSEKDLAYVRNLMTPTLEAGKIAHWISK
                     PTHGIHGLPFEFEYVRREP"
     gene            complement(19016..20677)
                     /gene="boxC"
                     /locus_tag="VFA54_01790"
     CDS             complement(19016..20677)
                     /gene="boxC"
                     /locus_tag="VFA54_01790"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HZS49565.1"
                     /translation="MPGTAMMEPAMVPGELETHPSRYKHWRLTREGPLACLTMAVAED
                     APLVPGYKLKLNSYDLGVDLELADAVQRLRFEHPEVRAVILTSGQERVFCAGANIHML
                     AAAPHAFKVNFCKFTNETRLAMEDASAHSGQHYIAACNGTTAGGGYELALACEEIYLV
                     DDGRSAVSLPEVSLLGVLPGTGGLTRLVDKRKVRRDLADVFATLPEGVKGRRAVEWRL
                     VDGVFPKSRFSESVQKRALDKARAETSAKGPGIVLDPLSPAVAEDSVRYRHVALHLNH
                     ARRTAEIIVNVPPGAHPRGVEEIHSAGADFWPLRAFRELDDALLRLRFHYQEIGLLLL
                     KTSGDIETVRALDAVLAENRHDWLVNEIILQMARVLRRLDLTSRSLFAIVEPGSCFAG
                     SLLELALAADRIYMLDGAGGARLATSPLNGGFLPMVHGISRLAVRFLGEPVKIEELLG
                     HKEPLDASAAENAGLVTAVVDAIDWEDELRMAVEERASLSPDALTAMEANLRFAGPES
                     PESKIFGRLSAWQNWVFSRPNAAGDHGALTLYGKPERPQFDWRRT"
     gene            complement(20826..22001)
                     /locus_tag="VFA54_01795"
     CDS             complement(20826..22001)
                     /locus_tag="VFA54_01795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZS49566.1"
                     /translation="MTLNRKIFYSTAIVAIVSLSVEGAARLIWRRLETRALQERHERG
                     EATLRNDAVNYLRVADGIYGYTLRPNTRAGAVYINGQGFHQREEIPVERRPGFLRIAC
                     LGESTTFGSDVDTNYPAYLRKILESSRAGFKGYEVINAGVPGWVSDQIALRVQHQLAA
                     FRPDAVFLYVGWNDFQSYDPFGPPPPVSYLEQAYGGASWKQYATSWLKSVALLSALYH
                     SGSRDAHEAAVSAARPVDNPPEQCYRFLLGSLGGIVAGFRRANPGVKIFLCTLVGRWP
                     QGTPHQWAHDPAVWWMDRHRVGPEQAAERLAIMNDQLRRLARRENLILVDDAQIFAPL
                     DRARLQWDWAHMYAEGYELLAWTMYSALHEAGIAPEAPSQRETELLAKYRLPPETRR"
     gene            complement(22110..22319)
                     /locus_tag="VFA54_01800"
     CDS             complement(22110..22319)
                     /locus_tag="VFA54_01800"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HZS49567.1"
                     /translation="MRKEQSEIHCAAPSVSAASGAEGWKAELWLFLRHNKKWWLTPII
                     VLLLLLGALVILGGSGEAPFIYTIF"
     gene            22548..22838
                     /locus_tag="VFA54_01805"
     CDS             22548..22838
                     /locus_tag="VFA54_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49568.1"
                     /translation="MESMAFESDKIMFEIYREKTYTGKYRVVYYTELGDHERDWEISR
                     AMAGESFYDGFLKNHRAEEGKQVIEQFLQRLNSGERVDPADVDQALRDYAAP"
     gene            complement(22884..23804)
                     /locus_tag="VFA54_01810"
     CDS             complement(22884..23804)
                     /locus_tag="VFA54_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZS49569.1"
                     /translation="MTPAAHLLSGYLVGEWQASGEKERWRAVAAAVVAGIAPDFDVVL
                     GLLGGWAGSGAHRGATHSLTLAPLFAWLIAKAVRGPRRPVFLAALGGIISHIFWDWLN
                     PWGAILFWPWMRSFRGNLVHEGNLYVLGLLLGSAVLVWRGRRVAAAVLLLELLPVFLL
                     VQIWWRDHARGLAAAQLAGRRALVYPAPDLACGWIALSAGSADMSVACVASPVARRLR
                     PVFTTPVRGDFFVQASERSPVVQDLREKIPFAFAEVRPLKNGGAVVLWRDLRVAYREP
                     KDADPTGLQVELDAHGKIVSEHYRWWLRLW"
     gene            complement(23822..25192)
                     /locus_tag="VFA54_01815"
     CDS             complement(23822..25192)
                     /locus_tag="VFA54_01815"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZS49570.1"
                     /translation="MPLSAQSFVDANRDRLLEELFEFLRIPSVSTLPEHRDDVRRAAQ
                     FVASRLGDAGMEHVEIIPTEGHPLVYADWLHAPGKPTLLCYGHYDVQPPDPLDQWVTA
                     PFEPAVRNGSIYARGSADDKGQMYIHIKAMEALRSLYGKLPVNVKFLIEGEEEVGGKS
                     IARFVPENKRKLAAGVALVSDTAMFQANMPTLCIGLRGLIYMEWEASGPSRDLHSGLY
                     GGAAPNAIFGLIELLSKAKDASGVIRIPGIYDDVKKPAPAELESWARLPFDEKEFLAK
                     EVGSTCLTGEKGYSVFERTWARPTFEVHGIAGGFTSPGAKTVIPAKATAKVSIRLVPD
                     QNPERVIEQVRNFVRTNAPAGVRVEVRVLSAAPATIVNPDHRAIRLAAQALTEVFGQP
                     TVFVRSGGSIPIVGDFAQRLGIPTVLMGFGLPDDGLHSPNEKFSLENYYKGILSVVHF
                     VEKLAA"
     gene            complement(25229..25756)
                     /gene="hpt"
                     /locus_tag="VFA54_01820"
     CDS             complement(25229..25756)
                     /gene="hpt"
                     /locus_tag="VFA54_01820"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZS49571.1"
                     /translation="MPSPEVEKILFTEDQIQKRIQEVGAEISEAFSGREIKLISVLKG
                     SIFFLTALARHITTPVKLDFLAISSFSSSPRAPGVVRISKDLDDTIEGEDVLLVEDIV
                     DTGLTARYLLQTLAGRQPNSLGVCTFLDKTPRRLVQVPVRFRCFEIPDRFVVGFGLDY
                     NQLYRNLPYVAVMKL"
     gene            complement(25931..26893)
                     /locus_tag="VFA54_01825"
     CDS             complement(25931..26893)
                     /locus_tag="VFA54_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HZS49572.1"
                     /translation="MAILTISAQPGLRADEVAQQVAAHLGFELVTESRVEALYKSELA
                     GPGGIPPRAHADLVISIVAQLAAQHHLVFAGRGGEFLFPHFPGLLRIQITASPAARAG
                     MLMVAQHLERPAALAALQQLDRENAAWLRARFRKFGRSRGRFDVSVNAERWDSAGLAE
                     IITAAARAARIEDAGLLSPAAERQLQFEVRLRLARMGLSGLRLNASLPARRFAHPSEE
                     VFANLLDFYRIPWEYEPRSFPLQWDEQGRVVESFTPDFYLPEFDLYVELTTMKQSLVT
                     RKNRKIKRLRELYPEVNVQVFYQKDFENLIFKYGLERTTEVPKS"
     gene            27143..>27860
                     /locus_tag="VFA54_01830"
     CDS             27143..>27860
                     /locus_tag="VFA54_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZS49573.1"
                     /translation="MSVVIGLDIGGTKLAGGVFSEDAVLRERRERPTRAEQGVAVSLE
                     EVYGLLAELVAAAAKHGPLAGVGICSPGPLDPKTGVVFNPTNMPGWVNIPLAAEIERR
                     SGVPCRVENDANGAGLAEAKFGAARGFSKVFYATVSTGIGTGIIFDGRVYHGKNGAAG
                     EGGHVSIDYRSPVRCNCGNFGCIEALASGTAIARRAREKIAAHPESKMLALAGNPGQI
                     SAAIVARAACLGDKLAIQLIE"
BASE COUNT         5602 a         8665 c         8241 g         5352 t
ORIGIN      
        1 ggttgccgta aatgatctga acgctgccgc tcgcgccggg cgtaacgcgg agcacgccgg
       61 gcacctggcg caggatcgct tcggcatcgt cagtgaccag cgtgcggatg ctgcccagac
      121 cgccgcgaat gccgcccatg ccccccgaac cggcattgat ttgcagggtg ttggttccga
      181 ggacggccat gcggtcctgc acctgagcct tggcgccctg cccgacgccc accatcgtaa
      241 tgaccgatcc gactccgatg attatgccca gcatcgtgag cacggaacgc atcttgtgcc
      301 gtgacagcgc gcggagggcc acccgaagga tcaccagcag gttcatagac gttctatttt
      361 cgcatcaagt ctgcgcagag aatcgggcgc cccgcctcca acgccgtctt tccggagcga
      421 ggttttccca aggcgcccac gggagcagtt cggccccggt cgaggcgtgg cgggcgctca
      481 agctgccgtt ttactcgtag cgtaaggctt cgatgggatc cagacgggcg gccttgcgag
      541 ccggatagag accaaaaaaa acgcccatcg cggcggaaaa cagaacggag acagcgatgg
      601 acacgagcga aatacggctc ggccactcga gaacggtgga aagaattttc gacgctgtca
      661 gcccgaaaat gatgcccaac aggccgccga acaggcttaa aaccatggct tcggtcagaa
      721 actgcgcctg cacgtcctgc tcggtggcgc cgatggccat gcggacgccg atctcccggg
      781 tccgttcggt caccgagacc aacatgatat tcataatccc tatcccgccc accagcagcg
      841 aaacggacgc gaccgaggcc agcatgagcg tcatgacctg ggaggattcg tcggccagtt
      901 gggcgacatc gacgttgttg cgcacaaaaa agtcatcggg gtcggtcggc ttgatccggt
      961 gccgctcgcg cagaaggtct tccacctgtt tttgcaccat ataaatcgct tcgcgcgaca
     1021 cggcctgcac ctggatgtag ttgagccagg tgatgcccag caccttcttc tgcgccgtgg
     1081 tgtaaggcat gaaaatgcgg tcatcctggt tgaaccccat gccgctttgg ccctgggcca
     1141 cggtgacgcc caccacccga aagggcagat tcttgatgcg caccgttttg ccgatggggt
     1201 tctcgtcgcc gaaaagctgc ttgcggacct cggctccgag gatgacgacg ttgttcccta
     1261 actgcacatc ttcatcggtg aagcaggatc ccagctccat cggcgtttcc ttaatctgga
     1321 agtaggaagg agtaacgccg tgcactcccg tttgccagtt ctgattgccg taaatgatct
     1381 ggacgctgcc ggtagcgccg ggcgcgacgg ccctgacgcc gctgacctgc cgctggatag
     1441 cgaacatatc gtccactacc agggtccgga tgctccccag ccctccatgc acgtggccgc
     1501 ctccgcccga accggcgccc accatcaaag cattggatcc caaggtggcg atacgctcct
     1561 gcacctggac attcgccccc tgcccgactc ctaccatggt gatgaccgag cccacgccga
     1621 tgatgattcc cagcatggtg agagccgagc gcatcttgtg ccgggacagg gcgcgcagag
     1681 caacacggag agtcacgagc aagttcatgc ctcttcctcc tcgtcatcgc caaccaattt
     1741 catttgcggc aaaacctcgg ctgcgatcag ccgtttctgg actttttcgt ccttccggat
     1801 cttcccatcg gcaaagatga ccgtccgctt ggcgtaagcc gcgatgtccg gctcatgcgt
     1861 cacgagaacg atagtcagcc cttcgcgctc attcaactgc tgcaaaatgt ccatgatctc
     1921 gaccgacgtg cggctgtcga ggttgccggt gggctcgtcg gcgagcagaa tggaggggcg
     1981 gttcatcagg gcgcgcgcaa tggcgacgcg ctgttgctgg ccgccggaaa gctggcttgg
     2041 aaaatggtgt tcacgcccgt gcaggccgac ctgccggagc gcctccatag cccgtgcgcg
     2101 cctctcctcg ctgttcattc cggcatagat accagggagc tcggtatttt ccagagccgt
     2161 cgtgcgcgcc agaagattga acccctgaaa gacaaagccg atatggcggt tgcgaatgcg
     2221 ggccagttcc gtcttggtca gacccgccac attggttcct tccaggaaat acgagccgcg
     2281 tgtggggcgg tccaggcagc ccagaatgtt cataaaagtt gactttccgc tgccgctggc
     2341 gcccatgatg gcgacgaatt cctggcggga gatgtcgagc gagatgccgc gcaaagcgtg
     2401 gacctgcacc tcgcccaagt catagatctt gtggacgtga tcgatgtgaa taacggtccc
     2461 gttggaaccg gggccgacct gcggagagtt gccgttgttg gatgccatcg ctgccgcccg
     2521 ctacctgggg ccgcgcatgc cgcccatggg cccgattccg ccacggccca gcatcccgct
     2581 aatgccgcga tttggcaact ccttgcccgt taccacctct tcccccggct tcaattcgcc
     2641 ttggaggatg gcagcgcatt cggtgtatgt gaaatccgtg ataccagtac gcacaaccac
     2701 gggacgcagg gtcttgtcgg gctgcaatac gtaaagagtc ctgatgtcct gcactgccgg
     2761 acggcgtacc ggggcttttc gctccggctc cgcgctggcg acttccggat ctcccccgcc
     2821 gccgccccca ccccgcttgc ctctcccggc gccagcgcgc cgcccttctc tacccccgtt
     2881 tccgcctcca ttaccctcgc cgtcaggcgc ggtcaggccg gcccggctcg cgccgcggtc
     2941 tttgaactgg tacttagcaa acagagcctg ccgctccggt tcctctaggc ggaaccgcaa
     3001 ggcgccgttg ggaatcttga tggcgttccg ggcttccgca acaggaatag tcacgtacgc
     3061 ggtcatgccc ggcaggagtt tctgttcggg attatcgaat tcgatgactg tgtcgtaagt
     3121 caccacgttc tggatggttt ggggattcat acggatctgc gacacgcggc cgtgaaaagt
     3181 ctccctcgga tacgcatcca ctttaaacgt cgcttcggcg cccacgtgaa tattgccgac
     3241 atcgctctcg tcggtcttcg catagaccag catcttggtc aggtcctgcg cgatcgtgta
     3301 aagcgtgggc gcttgcaagc tagcggcgac ggtctggccg acgtccacgt tgcgggcgac
     3361 gacggtgccg tccacgggag cgtaaatcgt cgtgtaagcc aggttgactt tcgccagctc
     3421 gagcgccgct ttcttctgct cgacctgcgc tttagcctgc gccaactgcg aaaccgccgt
     3481 atccaccatg gcgcggctat gctcggccgc ggccttcgcc gcctttgtac tggccaccgc
     3541 agcatcatag gtgctctgcg cctgatcccg aacctgagcg gcgacgattc cctgtttgaa
     3601 cagatccaga ttgcggtcat agtccagctt ggcctggcgc tcggccgcca tcgccttttc
     3661 cacgttggct tcggcgctgg ccaagtcggc cttcatcgtc tcgatgttgg cttcgagggc
     3721 ccgaacgttg gccgcggaat tttccagatc ggcctgcgcc tgctgaacct gagccttaaa
     3781 aatcgattgg tccaactgcg cgatcacctg cccctttttg acgcgcgagt tgaaatcgac
     3841 aaacagcttg tcgatgattc cggaaacctg cgacccgacc tgaaccgtga tgaccgcatt
     3901 gatggtgccg gtggcggtga cttccgaccg gatgtcgccg cgctcgacgg tagaggtgaa
     3961 gtagacaggg tccttcttgc ggctgcgggc gaaagcggcc agaagcgtgg cgccggccaa
     4021 tatcacccca acgacgagga ttcgctttga tgtcagaaag cgcgtgggct tcttctgctc
     4081 aattaaaggg gtatccatgc caataataag gcgtcggcag ccgcacactg gttacgctgc
     4141 cgccacgccc gacatggctt acggtaccac ccgggaaggc ggggtgcaac tccggttttg
     4201 tcaatcctgc cgcttggctc ccagctccga caggaccttt tggaagcgct tgcgttgatc
     4261 tgcgttcagg atgtgcagga tctggtcatg gcccgaagtg cggctatgct ggaactgctg
     4321 catgagattg gcctgttcca tgatcacctc gtccaggatg tcctcgatag ccttgacctg
     4381 ctcggagttg agatcgagtt cctgtgaaaa gtgatctacg gccatttgtt tttccgcctc
     4441 ggtcaaaacc ggacccgggc ggcggctccc ataatgctcc accagcttca gcgtcaccgc
     4501 gccggccagc attccagaaa cgaacacgag acccaacagc gcaaaaagct tccctttgca
     4561 gccggctacc ttgggatcca ccatggctca gtctccgtca gccaccaggg tggccagcat
     4621 gactccccgg ttccggtcca agctcggccc gatccttggc gcgggcgccg aggattcctg
     4681 gcccgccagg accgcttccg gcagatgctg gagcgcatag tcgggctttt caaaagccgc
     4741 cacgtaggtc cccaaaaggg ccagcaacat caaacacgca aaaaccagct tccggccgaa
     4801 ggcggggtcc agaagcatgg cccaaaaagg gatttcccgt tcctcatcaa tcctctccat
     4861 caccctcgcg taaaagccgg gcgccagatc ccagtccatc gccagcggcg ggcgcaggct
     4921 gcggatagcc tccgccgact ggcgcaacac aatccactcc tgccggcaac cggagcactc
     4981 tgccagatga ctttctactt ctcccaaagt ggcggaatcc agattgccgg agaggtagcc
     5041 ctccagatta tccctgagca ggacgtgcat aacctcttcc cccaatccga aagggccgaa
     5101 tcaacggccc gagcggcctg agccaggttc ccgcggatct tcgtccgctt cgggtacagc
     5161 ctatcacaaa gcccaggtct tgccgagtct gccagcggct tttaccagcc tttggcgggc
     5221 ccgaaacagc ttcaccttga tggtgttttc attgaggccg gtcgccgcgg ataattcctc
     5281 cacggtccgg ccctccactt ctttttcgat cagcagcatc cgctcttctt cgggaacctt
     5341 catcaacagc ttcaatatat aatcccgctt ttccagccgc tgatcgatcg gctgttcgcg
     5401 gtttaccggc gtctgtgtat tttccagccg cttcgactct tcctcgctca tgtcgctttc
     5461 gtaaacgagg cgccgcacct tttgcttgcg caggtaatcg tagcactcgt ttaccgtgat
     5521 cttgtaaatc caggtaagca gagaagaacg cagatcgaag ccgcggatgg aaaagtaaac
     5581 cttggtgaat acttgctggg cgatatcctc cgcgtcatta cggtggcgga caataccgta
     5641 aatgatggag tacaccttag actgatggcg ctccacgatc tcgcggaacg cgatctcgtc
     5701 gcctgcctgg caccgtttta ccagcgccgc ttccgagctg tcgtgcttct ccaccttggc
     5761 tttgttcgga gcctcagaaa gcgtagccgg taacgggaaa acgccggttg ccatacgacc
     5821 attccgacgc ggcgaggaca ttcagggttt cctaaaactg aatgaaaact cgcggctggc
     5881 aaccgaactt actcagaaat caccaaacta tcggcaagag gcatagcagc ccgttttccg
     5941 gtcctgacct ccccacgggt tttcactcag cgctgccaat tgtaccattt tgtgagattt
     6001 aggcgctgac aagcctgagt cggggcccgg gcaaggcaaa gcggccgtcc tccccgccgc
     6061 ccagccgcag atttggggcc aatggaggtt cgcgagccgg ggtttcctca gcctgctcgg
     6121 cgaggctcgt gacgaaggca ttcccgcctg cttttgccgc ctttcgccgc gctcgtttgc
     6181 gcggcgtgcc tccctgccac gccttgcatg ccgcaccgtc cacggcaggt tctcgctctg
     6241 cgagcgcgcc gcgatgatgc gcagctgccg ctctgccata atgagactgt ggactttaag
     6301 ctggtatctc cctacagccc ccgcggcgat caagtgacgg ccatcgagca gcttgtgcgt
     6361 ggcgtgatgg aaggggaggc gcatcaggtt ttgctcgggg tgaccggttc cgggaagact
     6421 tataccatgg ccaaggtcat cgagcgcatc aaccgcccta ccctcgtcct cgcccataat
     6481 aagaccctgg ctgcacaact ttataacgag ttcaagacgt tttttcccca caacgccgtg
     6541 gagtacttcg tcagttatta cgattactac cagccggaag cctacatgcc cgccacggat
     6601 acctacatcg aaaaagaggc cactattaac gacgagctgg acaagctgcg cctttcggcg
     6661 acgcggtcgc tcttcgagcg ccgagactgc ctgatcgtgg ccagcgtcag ttgcatttac
     6721 ggactgggat cgccggaggc ctattatggc atgctgctca tgctcgaaaa aggccaaaag
     6781 atctcccgga atcagatttt gagccggctg gtggaaattc tttatgagcg tagcgaaacg
     6841 gatttcaagc gcggcgcttt ccgggtgcgc ggcgacacca tcgagattta tcccacctat
     6901 gacgattacg cctaccggat cgagttgtgg ggcgatcagg tggagaacct gtatcagatc
     6961 gacccgctca ccggacaggt gcgccagagt tatctgcgcc tgcccatcta tcccaagacg
     7021 cactacgtca tgtccgacga tacccgccag aaggccatcc agagtatttt ggcggaactg
     7081 gcttggtgga aagccgagct cgaaaaggaa ggcaagtacc tcgaggccca gcgcctgttc
     7141 cagcgcacga tgtttgatgt ggagatgatg cgggaaatcg ggtattgcca tgggatcgaa
     7201 aattactccc gccatttctc cggccgcctg ccgggcgaag cgccgcctac cctgctcgac
     7261 tacctgccgc aggactcgct gctgtttatc gatgagagcc atcagacgat tccgcagctt
     7321 cacggcatgt atcacggcga ccgttcccgc aaagaaaccc tcgtaaaaca cggtttccgg
     7381 ctgccgagcg ccctcgacaa caggccgctt accttcgaag agtttgagca ccgggtcaat
     7441 caggtgattt atgtctcggc cactccgggc ccgtatgagc tgactaaatc ggcgggggtc
     7501 gtcgtcgagc agttgattcg cccgacgggg ctggtggatc ctcaggtcga aatccggccc
     7561 atccgggggc aagtagatga cctactgcac gagatccgcc gcgcggcgga gcgcaacgag
     7621 cgcgtgctgg ttacgacgct gaccaagaga atggcggaag acctcacgga gtactattcc
     7681 gagcttggcg ttcgctgccg gtacttacac tccgaaatcg agaccctcga gcggattaaa
     7741 atcttgcgcg acctccgcaa gggagagttc gatgtgctca tcggcattaa cttgctccgg
     7801 gaggggctcg acttgcccga ggtatctctg gtggcgatcc tggacgccga caaggaaggc
     7861 tttctgcgct cctcgggctc gctgatccag accatcggac gcgccgctcg ccatattaat
     7921 gggcgggctg tcttgtatgc cgaccggatc acggacagca tggcgcaggc cctggccgaa
     7981 acgaaccgcc ggcgcgccgt ccagatgcag tacaacgagg ccaacaacat cgttccccaa
     8041 tccatcctga agccgatcga tatgagcctg gtccaggtgg cggagggcga ttacgtaaca
     8101 gtgccgctcg aagccgagac cgatctggct tccctgactc cggaagaggc tgggcggtac
     8161 atctccgagc tcgagcaaag catgcgggaa gccgcccgga aatttgagtt cgagaaggcc
     8221 gctcaattgc gcgatcgcat aaagtcgttg aagacccgtg agattgtagg tacaatgcag
     8281 tcggccggct gacgatacgt tctatgctgc gagttggctt gatcggcccg gggcgggccg
     8341 gtcaggcgct ggtgcggctg ctaccgccgt cccactatga gatcggtcca gttctggggc
     8401 gtacgttcac gtcggccagg cgtgtcaccc ggcagatgcg tctgggtgag ccgaccgaga
     8461 gctacgagga tttcgcggcc tgcgatctga ttttcatcac agtgccagat gacgcgattg
     8521 cctcctcggc ccaggcttta gcgcgcgctg ctttcccctt ccgccgcaaa gtcgtcctac
     8581 atgcaagcgg cgcgcttgat agctccgttc tcgcgccgct ccgcgaacgc ggggcttcag
     8641 tagggtctct tcatcctttg cagacattcg gccggcgagt gttgtcgctg gccggaatac
     8701 acttcgcagt ggaaggcgat gagacggcta tccggctggc gcaatcactt gtcactcact
     8761 ggcacggcaa gctgctgcgc atcaaggcgg accagaagat tttctatcat gccgccgcca
     8821 cgtttgcatc cccgctgttt acggccttgc tggaagccgc cgtgcggctg atgggccgcg
     8881 ccggagtcag gcccaaaacc gccattcggg cgctgcgccc tctgctggtc acaaccttcg
     8941 aaaattacct ccacaccggc aagcagagct ggactgggcc gctggcgaga ggcgatgccg
     9001 ttacagttcg ccggcacatc aaggccttgg ccgaatacga tcccgatcta gcttgctatt
     9061 atcgtgcggc gtcggtctcg gccctctccc ttttcagccg ccatccggaa ttgcaaatcc
     9121 agctcagaag acttcgtatc cgatgaagca ttggctgcgg caagacgcct tacacgcatt
     9181 tctttggctg gcggcatcgc ttctacttgc cgccggctct ctggccgcca agaagaagcc
     9241 gcaagcgccc gttgatatca acaccgcttc ggccgccgaa ctggcgcaat tgccgggcat
     9301 cggaccttcc cgcgccgccc agattgttcg tttgcgtgag aaaaacggtc ccttccggag
     9361 cgtggacgag ctccgcgcct tgccccgcct cagcgaaaag cagtttctcg aactgaagca
     9421 atacacgacc gccggttcct ccccccgccc aaagcccaac gccgccaacg cccagccgca
     9481 acgctgatgc agccgcctaa gacgggggcc aaggcccagg gaattttagc cgatgtaccc
     9541 cctccggcgc tgataacgtc tatactggca acatcgaaag aagggctttt atgaggcgca
     9601 tccctggaat caacacggga ctcggttgcc tgatccttct gctgtgcatt gccttacagg
     9661 acttcccggc cccagctcag attggatatc ccggcggggg ctatcccggc ggctatccgg
     9721 gaggataccc gccaggaggc tatcccccgg ggggctaccc gcccggcggc tatccgggag
     9781 gcggatatcc ttacccgggt ggcggcggag tgggcttccc cgccccccgg cggcgcggca
     9841 agcaagatca gactaaaaac aataaagatg ccgaggaccg ggtcaatgtc tcgggcctgc
     9901 ttcgcaagct ggacgattcg tccattatcg tgcaagccca ggacacccgc atcatcaccc
     9961 tgaagcgaac gagccagacg aagtttcaga ggaactccaa agacattagg ccctcggttc
    10021 tgaagcccgg cgatcacttg cgcgtggaag cccatcagga cgatcaaggc tacctctatg
    10081 ctttgacggt cttcctcgaa aaacagggaa ccaatgagga gcgggccgcc gcatcccaac
    10141 cggtcgaggc ccaagtctcc cccaccggta cgcaaggtga cccggatact ccgggtttgc
    10201 gcggaaccga gccggcgcac gccgatgatc cggatatccc cgtgtttcgc aggaagaccc
    10261 cgaaggattc accagccccg cccgattcgg cggactctgc aactccaacc gcgggcggaa
    10321 ctcctgccgc gccggcgcag gcgggcatcc cttcctcgga tgacgttgct tctacaacta
    10381 agattgaaag tcccccatct ctgcccaatg aagacgactc cgacagaccc cgcctgcggc
    10441 gcggctggtc acggggaaag ccgttgcctg attcggataa cggcgtacag gccgcatcca
    10501 accggccggc tatgaaggag cctatgcctg gaaccggggc gcgggaaccc gacgaaaccg
    10561 ctgccgcgcg cagctcggat ccggtgatcg aaaaggcacg ggcggcggcg gcggaattca
    10621 cggaaacttt gcccgattat gtttgtgaag agcaaatgac gcggtttgtc aacgtgtccc
    10681 atgtagtgaa ctggcagccg gttgatattg tgtcgagcga agttgtgtat gaaaagggcc
    10741 gcgagcgcta ccgcaacctt aaggtcaacg gcaagccctc aggcaaaagc atggaagaac
    10801 tgggtggcgc ctggtctacc ggcgaattcg gatcggttct ggtcgatttg ctttcacccg
    10861 ccacagcagc cgagttcgag ttccgccgcg agtcctcggc ggccggccag ccggccctgg
    10921 tctacgattt ttctgtcgag caggagcact cgcattggcg catccaggtt ccttctcaga
    10981 ctgtgattcc cgcgtacaag gggtcggtgt ggatcgacaa gaagacaaac cgggtgctgc
    11041 ggattgagat gcagacccgc aacatgccca gggagtttcc gctcgacaaa gtggaatcgg
    11101 ccgtggatta tgagtacgtg cggctggggg gcgaaaaact gtacctgctg ccggtgcacg
    11161 ccgagagctt gagctgccag cgcggcacca gccagtgcag ccgcaattcc atcgatttcc
    11221 ggaactacca caaatacacg agcgaatcgg aaatcacgtt tacggacccg aagtaagcca
    11281 aaaaacaggc tttcctcgcg gctgccaggt tctagtttgc cttctgcgtc cggcgcgcca
    11341 cgcgctccac ctctcgcatc acccgcagcg tattgccgcc gagaatcttt tcgatgtcgg
    11401 cgtcggagaa gccgcgttcc atcaggccct ggacaagatt cggaatcttg gagatatctt
    11461 ccatgccttc ggggaattga tcgttggctc cgtcgaaatc tgaacccaag ccgacgtgat
    11521 ccaccccggc caccttcacg gcgtgctcga agtggtcgag cagcctgctc agaggcacgc
    11581 gccccagcct ctccaacctt tccgcgtcga ttttgcgctg ctcggcggcg cggcgggccg
    11641 gatcaccggc aaactcctta ttgatggctt cttcgcgcgc attcaggtct ttcagcgcgc
    11701 ttgagcgctt cgcaaaatcc ggatcgagaa aggcattgta gaaattgatg tgcacgacgc
    11761 cgccgttttt cgccaaagcc cgcaacatat cgtcggtcat atcgcgggcg gccccgccta
    11821 acgcccggca tgaggagtgc gaggcgatga ccggagcgcg ggagacggcg agcgtgtcgt
    11881 aaaaagtttt gtccgagacg tgagaaacgt cgaccatcat gccgatccgg ttcaactccc
    11941 gcactacctg ccggccaaaa tcgttcaatc cgtccggctc cgatttgccg ccggaagagc
    12001 cggcccaggg agtcggcgtg ctgtgcgtca gcgttagata gcgcgctccc agcgcatagt
    12061 acgtgtgcag aatggctaga tcggagtcga tcatatggcc gccttccacc cccatcagca
    12121 tggcaatgcg cccttgcttc tttgcagcga cgatctcatc ggaggacgtg gccaggacta
    12181 ggtccgcagg atgccgggca atctcgcgcc ggtcggcatc gatctcatcg agcgcccgtt
    12241 tgatggattg caggggcgtg taccgcgccg agtcggtcca gatggaaaga aaaatgccag
    12301 acacgcggcc gcggcgcatg cgcgggatat cgacctggcc gtagtcatgc agctcgccta
    12361 agttataccc ttcgtccaga atcatctgcg tcgtgtcgtc atgcagatcg agcacgacgg
    12421 cgcgatcgag caagcgttga gcgcgcgcgg aaacggtggt ttgggccccg gcggacgcag
    12481 ccgccaatac caggggaatc agcgcccgca taggtcctag atgccgcaag gaagagctcg
    12541 gggcgtaggc gcggccgcgg aggccatcag cgggcaagat tcgctggcta aaagcttgtt
    12601 cgagcgggcc agcaggatga tctgccgcgc gccgtgctcg atcaggcacc gggggcagct
    12661 cagataccag aagtacatag actcgatccg ccccaccccg gcggccctca gatcgcggta
    12721 gaacctgggc atttcgcgca acgcagcgtc gaaaccgggc tcatcgacga cccgggaata
    12781 aaaaagattc tgaaagcttt gaatctccgg cgatgaacgc gggtgcggat ccaccgaaac
    12841 cagcacctcg ccccagaacc atccgtcacg atgtagaatc agtggggcgc cggcgggcct
    12901 cagccctagc cgcctgattt gattttccag ggcctccacc tcgcggtata aacgccgcgg
    12961 gacgtgaaga aagatggggg tctgcgccga aaggaattga tgaccggaca gcgtcacctc
    13021 gcgatcccgc caatccgcga ggtcaatctt gggacactgg cactccccgc ctgccataat
    13081 ttaaattata ccggacccga acacccgggg ctccaaacgg cccctcgaaa gtcgcctcgt
    13141 ccacccgtga ccggcagggg ccgcacagcc ggacgtcgga gaccggatgg ccatacccct
    13201 ctgctcccgc acggcagaaa gaccggcgag tgaacgcttg cccggcttac gacggcttcc
    13261 ggtacgagcg gcgtgactta gggagcatcc cgccggccgg ccaggctcgc gccggctgcc
    13321 cgaatggaaa tcgctggcgg cggctcggct gctctcggcc gccattctgg ttgtctttcg
    13381 cggcgcttac tggaggcctt tcgatgacca ggggtaggaa tcggtcgccc cctttcacgg
    13441 ttatggtctc aaactgctta ctcccggcct cgaggatcgg ccgtgccatc ccctgcccgc
    13501 cgccagggcg caaacaacag ccgccagcgc gaggccgctg caatcgagcg gcgcgccgat
    13561 cttgcgggca aaaggcgtca atggaacttg ttgcagcaac gcccttacac taccaaagga
    13621 atttcaggct gagctgcccg atgcgcgggt cccgggcgct gctcacctgg ccaaatcggc
    13681 cgttgctgaa attaccttgt ggattgttga actgcgcatg attgaacagg ttaaagaact
    13741 ccgcgcgaaa ctgcagcgcc atttgctcgt gcaagaccgt gttcttgacc aaggcataat
    13801 cccagttgtt gaaccctggg ccgtggaaga agcgccggtt cgcgtttcca aaaccaccga
    13861 gcgggccggt agtgaaagct gttttgttga agtacgtgtt gggcttgccg tctgcgccgg
    13921 ggtttctggg atcttgcatc gtgagaggtc cgacaaagtt tggcacatca atgcccggcg
    13981 ctcccaccag cgagcgatct ccggactgtg aaatggtcac cggcaggccc gtggcaaaac
    14041 gcgtaacgcc ggcgatgttc cagccttggg tcaagccctt gggagcgccc ctgaaaagcc
    14101 ggccgaaagg aatcgcgtag ctagtgctga aaacaaagtt atgggtgatg tcgaactgag
    14161 acagagaacg gcttaaagca tagttggaga agttgactcg attgccgaag ccggaagcat
    14221 tgtccatcga cttgccgaag gtataagccg ccagaaacgt gaaatcgctg gcccgccttt
    14281 ccagggtagc ctgaaaggaa ttataggaag agttagcgag cgtggattcg taatcgttgt
    14341 gcccgaagtt aaacccgaac ggcgcgcgcg tgccgaagac ctgcgaacca tcaggccttg
    14401 tgaagatccc gttctcctgg tttgggccgc actgcggcgt cccggccttc accccggacc
    14461 cacgcaggct caagcagagc gccggattgc cgggattgga ttcgacctcc gcgatcaact
    14521 tgtgcccctg tgttcctaca taagccagcg tgagaaccat cgaggaagaa agctgcctct
    14581 gaatggagaa attgtagtgt gaagcgtaag gcaatcggtt ggtcactgca tatcccggag
    14641 agcttgcaat cggcagaaag acggaccagt ccatggtctt gagcgaagga tcgccgggtg
    14701 tcggcagaat gaacgggaag cgctgctgct gcgaactgcc gtcagaccgg gtgcggaatg
    14761 gctcattgaa aaggggaggc acgctgctta cccagaaaag accatacggg gcgtcggcca
    14821 cgatcacaaa cagcgtcgca tcctcaatcg ccgtgtagta caacccatac gaagcgcgaa
    14881 tactggtttt ccctggcccg cctagcaatt tgccccatac gcctccggag acgtttggcg
    14941 accaggcaag ccccgcgcgt gggccaaaat tattgtagtc ggtcggcgcc agcgtccgcg
    15001 gaactccggg atcgcccggc accagccacc ctttgggagc cgtgggaaac acggtcgact
    15061 gcacgcccgg aatcatggtt tcaatcttgt cctgtgtgtc gtaccagggc tggctgaatt
    15121 cccagcgcag cccgtaattg aacgtcaggt tcggcctgat ccgccaggag tcctgtccgt
    15181 agatcccggc gtacttagtg cgggagtcca gcacctggaa cgaggcctgt atgtattggc
    15241 tgggagcccc cagcagaaag tccgcgatgt cactgccggt ctccgagccg tcaaaactga
    15301 agttcccgtt aggagcgtaa atgttccgct cgttgatttg cagataacga aactctccgc
    15361 cgaacttgaa cgagtgggtc gtgtggatct tcgagaagtt ttcggaggcg tgccacgtgt
    15421 tgttgaattg cccggtcgtg ccaatcgtcc gccccaaggt gaagttgatc agcgagatgg
    15481 gcggtacggc ttcaaagttt ggcggtccgg agggaacgat ccccagcgta ttcactcccg
    15541 tgataaatcc gaggctggat agcgacacgc cggggtctcc cggctgattg gcgtgcgtcg
    15601 ccgtcctcgt gaaactaagc cgggcctcgt tgactgcgct cgggccgaag gtgcgaacgt
    15661 tgcttaacac cccctgctga gcgcgtgtgg gcgtgctgct cggaaagccg ggaaagctgt
    15721 cggtgcccaa gggattgctg attgtggagt catcaaaata gtagtacgcg taccagttgc
    15781 cggtttttct gttcagaaaa tccacgcgct gtcccgcttt gtcatcaccg atccggctgg
    15841 cttggctgga atcagcgaac gtattcggcc ccgcgttggg aagaggaata tacttaagca
    15901 ggttggccgc aatcggcgaa aaagcgcggg ccgggattac gccgttcggg aacacgcagg
    15961 tggccgtgct cgtacatccc ggcaggctgt aaggctcgtt ggccgtgacc gtatagccca
    16021 gacgctgcga taacaccccc gcccaatacg agccgttgac agtgccattc aacgctccgg
    16081 cgccgatatt tcccgagcgt tgatcgagtg tggggacctg tatcagcccg gtcgaggctc
    16141 cctggctctg gcgagtgccc tggtagtcgg tgaaccaaaa cagcttgtcc ttgatggccg
    16201 ggcctcccac tgcatagccg aattgatttc gctttaaaat ccccctctgc ggatcaaaga
    16261 agccacggga atcgagcttg tcatttcgaa ggaactcgaa cgccgttccg tgcacgctgt
    16321 ttgaccccga cttggtaatc gcgttcataa ctgcgccgct gaagcggccg tattccgcgt
    16381 caaagctgtt cgtaatcagg cgaaactcgg ccactgaatc cagattgggg ataatcgcgg
    16441 tgccgaagtt tctgccctcg ctgacgtcgc caccgttcac caggaaggcg ttcgacgatt
    16501 ccctctgccc attgacggac acatttcctg cggagagttg cccggatacc gccgcgcttc
    16561 tggatgcccc ggagttgacc ggcaccacgc cggcttgcaa gccgagcagg tcaacgaagc
    16621 tccggccgtt cagaggcagc gacaaaatct tcttgtcatc aatcacctgg cccatttgag
    16681 tgctcgcggt ctccacctgg atcggattcg ccgtcacttc tacctgctgc tcaaggctcc
    16741 ccacctggag attgatgtgc acccggcgct gctcgttgac cgtcaggaca atgtcatcgg
    16801 cgacaaactt ctgaaacccg ctcagcgtcg cttccaactt atacctcccg acaggaagcg
    16861 ccagaatgcg gtactggccc acggtgtcgg tcaccgtcat gcgggtcagg tttgtatcaa
    16921 gattggtaac gctgatttgc acccctggca aagccgcgcc cgatggatct gtagcaatcc
    16981 ccagaattgt cgccgtcacg tcagcccata gcgacggccc atcgattgcc ggcaaaagag
    17041 tcaggagcat ggaatacaaa aggaaacgtc gcaagaattg tgttctcata acgaatccct
    17101 cagatatgct tgcgagcgcc cggctctgga caagccgcaa ctgcgaggcc tttgctctcc
    17161 ccgaagagca cattgatctt acctttggtc cggtgcgttt tcaacccaga tttccatttt
    17221 ttaagttccc cagcacggct gagtatgcag cagccgctat tcctttggac cccgtgaagt
    17281 tggatttccg ccgtggggga tccgccttcg ctcccgtcaa ccgctgcttt cgccttcccc
    17341 agaattcgaa accgacaagc cccatttttt cagctatttc cgtgcaaagc tctccgaatc
    17401 cctccgctcg tgacttcgct cgccagtagc ctccgtctaa ccggacaagc ttcgcgacgg
    17461 cagccgggcg gtggccgctt ctacaacgag caggggacgg ctgttacgag ccacgaacgt
    17521 cagccgagcc gaaacgagcc tcaccgctgg cttgcccagc cggggccacg cggcgcggcc
    17581 gcagatcccg ccgctatggc tcgcgccgca cgtattcaaa ttcgaatggg agcccatgga
    17641 ttccgtgtgt gggtttcgag atccagtggg caattttgcc ggcctccaga gtaggagtca
    17701 tcagattcct gacgtacgcc agatcttttt cgctgggcag aaactcatgg cggcgcgatt
    17761 cccattgctc agccgtcatg gggcgcccct gcggatcgaa gcgcccggcg ctgtaaacgc
    17821 caatccgccg gtgaaagcgg cggtccggga gccgcagcct gtacggaatg ccaaagccgg
    17881 cgatggtacg gttccatcga gccagtgcgc tctcgcaatc cgcaatgtaa gcatcgcgga
    17941 gcacctcatt catggcgttc cgcaaaggca cttccttctg ctcccatccg tttggcccgg
    18001 cctccagaat ccggtatgac ccttccaggg ccagatggtc ggaaaacgtc tcttccttat
    18061 aacggccctt caggccggcg gcgaaaaagt tggccgcatt gctcgaaatt tcaccgccga
    18121 aaaggtccag actgacgctg taccaaaggt taatgtagcg ctgcaccgtt tctagatcga
    18181 tcgcgccctg ccggcgcacg tcgtcgtcgc ccgtctcccg catgatttca caggcgcgtt
    18241 tgacaacgcg gtccacaccc gactgcccca caaacatatg gtgggcctct tcggtcagca
    18301 tgaagcgggt ggtccgggaa agcggatcaa atccgctctc ggcgagcgcc agcaactggt
    18361 acttgccatc gcgatcggtg aacgtcgtaa acatgtaaaa cgccagccag tcatggatag
    18421 gttcattgaa agcaccgaga atgcgcggct tatcggcgtc gccgctgcgc cgctcgagca
    18481 gttcttcggt ttcttcacgg ccctcgcggc cgaaatagga atgaagcagg tagaccatgg
    18541 cccacaggtg ccggccttcc tcgacgttta cttgaaacag gttccgcaga tcgtagagac
    18601 tgggcgtagt gcggccaagc agcctttgct gctccacgct ggccggctcc gtgtcgccct
    18661 gcgtgacgat gatgcgccgc agcatgttgc gatattcgcc gggaacctcc tgccatacgg
    18721 gctgtccgta atggtcaccg aagccgattc tgcggtcctt ctccggcggg gccaggaata
    18781 tgccccagcg gtattcgggc attctcacat aatcaaagtg cgcccagccg ccgggctcga
    18841 ccgaaaccgc tgtgcgcaga taaacccggt cggcctggaa tcccgccggg cccatctcct
    18901 gccaccatcg catgtaattg ggaagccagt gctccagggc gcgaaccagc cgcttgtcgt
    18961 tggacaggtt cacattgtta ggaattttgt cgtagagaga cataccccat cctcgctacg
    19021 ttctgcgcca gtcgaattgc ggccgttccg gtttgccata gagcgtaagc gccccatggt
    19081 ctcccgccgc gttcggccgc gagaaaaccc agttctgcca ggccgagaga cgtccgaaaa
    19141 ttttactttc cggagattcc ggaccggcga aacggagatt cgcctccatg gcggtgagag
    19201 cgtcggggga gaggctggcg cgctcctcaa cggccatgcg caattcatcc tcccaatcga
    19261 ttgcatcgac tacggcggtc actagacccg cgttttcggc cgccgaagca tccaacggtt
    19321 ccttgtggcc gagaagttcc tcgattttga cgggctcgcc gaggaatcgc acggcgagcc
    19381 gcgaaattcc gtgaaccatc ggcaggaagc ccccgttcag cgggctcgtg gcaagtcgcg
    19441 ccccgccggc gccgtccagc atataaatgc ggtcggccgc cagcgccagt tccagcaggc
    19501 tgcccgcgaa gcaggaccca ggctcgacga tggcaaagag actgcgggac gtaagatcca
    19561 aacgcctgag cacccgcgcc atctggagaa tgatttcgtt gaccagccaa tcgtggcggt
    19621 tttccgccag gaccgcgtcc agggctcgca cggtctctat gtcgccgctg gttttgagca
    19681 acaaaagccc gatctcctgg taatggaacc gaaggcgcag caaggcgtca tccagttcgc
    19741 gaaacgcgcg caaaggccaa aaatcagctc cggcgctgtg gatttcctca acgccccggg
    19801 gatgagcgcc cggagggaca ttcacgataa tttcggccgt ccgccgggca tgattcagat
    19861 gcagggcgac atgccggtag cggacagaat cctctgctac ggcaggcgaa aggggatcca
    19921 gtacgattcc cggacccttg gcggaggtct cggcccgagc cttgtcgagt gcccgcttct
    19981 gcaccgattc gctaaaccgg cttttaggaa atacgccatc caccagccgc cactcgacgg
    20041 ctctccggcc cttgacgcct tccggcagcg tggcaaacac gtcggcgaga tcgcggcgaa
    20101 cctttctttt gtccaccagc cgggtgagcc cgccggtacc gggcagtacg cccagcagcg
    20161 acacctcggg caggcttacc gcggagcgcc catcgtcgac caggtaaatt tcttcgcacg
    20221 ccagggcaag ctcatagccg ccgccggccg tggttccgtt gcacgccgcg atatagtgtt
    20281 ggccgctgtg cgccgaggca tcttccattg ccagccgcgt ttcattggtg aacttgcaga
    20341 agtttacttt gaaagcgtgc ggcgctgcgg caagcatgtg gatgttggcg cccgcgcaaa
    20401 aaacccgctc ctgcccgctg gtcagaatca ccgccctgac ttccgggtgt tcgaatcgca
    20461 gcctctgcac tgcgtcggcc aattccaaat cgaccccgag gtcgtaggaa ttgagcttca
    20521 atttgtagcc gggaacgagc ggggcgtctt ccgccaccgc catggtcaag caggctaacg
    20581 gcccctccct ggtcaggcgc cagtgtttgt agcgacttgg atgagtctcc aactcgccgg
    20641 gaaccatagc tggttccatc atagcagtcc ccggcatgag gtatccgagc cgccacgcac
    20701 agaaccgcgc cgtcgaatgg gtggctgcct tccgttacgc aattcgatag cagagcttca
    20761 gcagtctcca gcgggcagat tggcaaagaa acggcggttt cccgcagcgg cccttgcccg
    20821 tcgtgtcagc ggcgcgtctc cggcggaagc cggtatttgg caagcagctc cgtctctctc
    20881 tggctcggcg cttccggggc aattccggcc tcgtggagcg ccgaatacat ggtccaggcc
    20941 agcagctcgt agccttccgc gtacatatga gcccaatccc actgcaaacg cgcgcggtcc
    21001 aagggagcaa aaatttgggc gtcatcgaca agaatgagat tctcgcgcct ggcaaggcgc
    21061 cgcaactggt cattcattat ggcgagccgc tccgccgcct gctccggccc aaccctgtgc
    21121 cggtccatcc accacaccgc tgggtcatgc gcccactggt gcggagttcc ctgcggccag
    21181 cgtccgacga gcgtacacag gaagatcttc acgccgggat tcgcccggcg aaagcccgcc
    21241 acgatgccgc ccagactgcc cagcaggaac cggtagcact gctcgggagg attatccacc
    21301 ggacgcgccg ctgaaacggc ggcttcatgc gcatcacggc tgcccgagtg gtacaaggcc
    21361 gaaaggagcg cgaccgattt cagccagctt gtggcatatt gtttccaact cgcgccgccg
    21421 tatgcctgct ccaggtaaga aaccggcgga ggagggccga aaggatcata ggattgaaaa
    21481 tcgttccagc ccacgtagag aaaaacggcg tcgggccgga acgcagcgag ctggtgctgc
    21541 acgcgcaagg cgatctggtc gctcacccag cccggcacac cggcgttgat aacctcgtag
    21601 cccttgaacc cggctctcga ggactcgaga attttgcgca gataggccgg atagttcgtg
    21661 tccacgtcgc ttccgaacgt ggtggattcc cccagacaag cgatgcgcaa aaaccctggc
    21721 cggcgttcca ccgggatttc ctcccgctgg tgaaatccct gtccattgat atacacagct
    21781 cccgcccgcg tgttaggacg cagcgtgtat ccataaatgc catccgccac gcggaggtaa
    21841 tttacggcgt cgttgcgcag cgtcgcctcc ccgcgctcgt gccgctcctg aagggctcgg
    21901 gtttccagac gccgccagat caggcgcgcg gctccttcga ccgacaggga tacaatcgcc
    21961 acaatggcgg tcgagtaaaa gattttccgg ttgagagtca tggcgccgga ggatccgcgg
    22021 ctggcgctgt tcctgcttct acccgggaac cggggcttgg cggcctgggg cgaatacggc
    22081 gccgcggcaa aacgccgccg accgaggcgc taaaaaatcg tgtaaatgaa aggcgcttcg
    22141 cccgagccgc ccagaatgac gagcgcgccc agcaacagca gcagcacgat aatcggagtg
    22201 agccaccatt tcttgttgtg ccggagaaag agccaaagct ccgccttcca tccctcagcg
    22261 ccgctggcag ccgaaaccga aggcgcggcg caatggatct cgctctgttc cttccgcatg
    22321 acaaatcact ggcgaccggg agggtttgag cgccctgccc gcacaagcga gcataatagc
    22381 tcacgcagcg ccggccccgc aatggccgac gggcgttgtg agcggcagcg accgtctctc
    22441 acgaggcggc aaatatctgg attgtgcggc gaaacgagtt catacctcgc gccagccgcc
    22501 gattcgcacg gccggcggtt ccggaggtag gcctagcgga tgctagtatg gaatccatgg
    22561 cgtttgagtc agacaaaatc atgttcgaga tctatcgcga gaagacttac accggaaaat
    22621 accgggtcgt gtattacacg gagctcggag atcacgaacg cgattgggag atcagccgcg
    22681 cgatggccgg ggagagtttt tacgatggct ttctgaaaaa tcacagggcc gaagaaggca
    22741 agcaagtgat cgagcaattt ctccagcgcc tgaacagcgg agaaagggtc gatcccgctg
    22801 acgtggatca ggcgctgcgc gattatgccg ctccctgaag cccagcggcc tggtccctcc
    22861 gctggaggcg gcgcgaaagc gcctcaccaa aggcgcagcc accagcggta gtgttcgctc
    22921 acaatcttgc cgtgtgcgtc caactccacc tggagccccg tggggtctgc atctttcggc
    22981 tcgcggtatg caacgcggag atcgcgccag agcacgacag cgccgccgtt ttttagaggg
    23041 cgaacctcgg cgaaagcaaa tggaatcttc tcccggagat cctggacgac gggcgagcgc
    23101 tccgaggcct gcacaaaaaa atccccgcga acgggcgttg taaaaacagg ccggagccgc
    23161 cgagctactg ggctggcgac gcaagctacg ctcatgtcgg cgctgcctgc ggaaagggcg
    23221 atccatccgc aggccaggtc cggcgcagga tagacgagtg cgcgccggcc ggccagttga
    23281 gcggcggcca ggccgcgggc gtggtcccgc caccagatct gaaccagcag gaatacgggg
    23341 agcagttcga gaagcagcac ggctgcggcc actctgcgcc cgcgccacac cagaacggct
    23401 gaacccagca gcagacccag tacatacaaa ttgccttcgt gaaccaggtt gcctcgaaaa
    23461 gatcgcatcc agggccagaa gagaattgcg ccccaggggt ttagccaatc ccaaaaaata
    23521 tggctgatga tgccgcccag agcagcaaga aaaaccggcc gccgggggcc tctcacagcc
    23581 ttcgcgatca gccaggcgaa aagaggcgcc aaagtcagcg agtgtgtggc cccgcgatgg
    23641 gcgccgctgc cggcccatcc tcccaacaaa cccaatacca cgtcgaaatc cggcgcaatg
    23701 ccggcgacga cggcggcggc gacggcgcgc cagcgttctt tctcgccgct cgcctgccat
    23761 tcccctacga ggtatcccga aagcaagtgc gcggccgggg tcatgggatg gcgatgggct
    23821 gctaggcggc gagtttctcc acaaaatgga cgaccgacaa tatccccttg tagtaatttt
    23881 caagagagaa tttttcgttt ggggagtgca agccgtcgtc gggcagaccg aagcccatca
    23941 gcacagtggg gatacctagg cgctgggcaa agtctccgac aatcggaata gatccgccgg
    24001 agcggacaaa aaccgtcggc tggccgaaaa cttccgtcaa cgcctgcgca gccaggcgga
    24061 tcgcccggtg atccgggttg acgatggtgg ccggcgcagc gctcagaacg cgaacttcca
    24121 cccgcactcc ggccggcgcg tttgtccgca caaaattccg aacctgctcg atcactcttt
    24181 cgggattctg atcgggaacc aggcggatgc tcactttagc agtagccttg gccggaatca
    24241 cggttttggc tccgggggag gtaaagccgc cggcgatgcc atgcacctca aaagtcggcc
    24301 gcgcccacgt gcgttcaaaa acgctatagc ctttctctcc tgtcagacaa gtggatccca
    24361 cctctttggc aagaaactcc ttttcatcga aaggcagcct ggcccagctt tcgagttccg
    24421 ccggagcggg cttctttacg tcgtcataga tgccgggtat ccgaatgacg ccgctcgcat
    24481 cctttgcctt agataacagc tcgatcaggc cgaatatggc attcggcgcg gcgccgccat
    24541 agaggccgga gtgcaggtcg cgcgaaggtc cgctggcctc ccactccatg taaatcaagc
    24601 cgcgcaggcc gatgcaaaga gtcggcatat tggcctggaa catggccgtg tcgctcacca
    24661 gggctacgcc ggccgccagc ttgcgcttgt tttcgggaac gaaccgggca atggattttc
    24721 cgccgacctc ctcttcccct tcgatcagaa atttcacatt caccgggagc tttccataaa
    24781 ggctgcgcag cgcctccata gccttgatgt ggatgtacat ctgcccttta tcgtcagccg
    24841 agccgcgggc gtaaatgctg ccgttccgca cggccggctc gaagggagcg gtgacccatt
    24901 gatcgagagg atcgggaggc tgcacgtcat agtggccgta gcagagcagc gtgggcttgc
    24961 ctggggcgtg cagccagtcc gcgtagacga gcgggtgccc ttcagtcgga ataatctcga
    25021 catgctccat gccggcgtct ccgagacgcg aggcgacaaa ctgcgccgcc cgccggacgt
    25081 catcccggtg ctcgggcaga gtgctgacgc tcggtatgcg caggaactca aacagctcct
    25141 ccagcaggcg atcgcggttc gcgtcgacga aactctgggc gctcaggggc attgcctcct
    25201 ccaggagaag accatcatta tacgccgctc atagcttcat tactgctaca tagggaaggt
    25261 tgcggtagag ctggttgtag tcgaggccga agcccacgac gaagcggtcg ggaatttcga
    25321 aacaccggaa acgcacgggc acctgcacca gacggcgggg agttttgtcg agaaacgtgc
    25381 agacgccgag gctgttcggc tggcgcccgg ccaaggtttg cagcagatag cgggccgtca
    25441 gcccggtatc gacaatgtcc tcgaccagca gcacgtcttc tccctcgatc gtgtcgtcga
    25501 ggtctttgga gatgcgcacc acccccggcg cgcgcgggct gctcgaaaaa ctggaaatcg
    25561 ccagaaaatc cagtttcacg ggcgtcgtga tgtggcgcgc gagagcagtg aggaaaaaaa
    25621 tcgagccctt cagcacgctg atcagcttga tttcgcgccc ggagaaagct tccgaaattt
    25681 cggcgcccac ttcctgaatc cgcttctgga tctgatcctc ggtaaagagg attttttcga
    25741 cctcgggaga gggcatcatc aagagccatt atagcgggcg gctgttgttc gcacggttct
    25801 cgaagatggt ggtgcggacc tcaccggcga gcgaggagcg agcgccggca gaagcgcgtg
    25861 ctgcgccgcc agattgttcg cccaggcggc gaatcctgag gaagaatgtg gttcgacgtc
    25921 ccgggctgcc ttaggatttc ggaacctcag tggttctttc caggccgtat ttgaaaatca
    25981 gattttcgaa gtccttctgg tagaagacct gcacgttaac ctcaggatag agttcgcgga
    26041 gccgtttgat cttgcgattt tttctcgtca ccagcgactg cttcatagtc gtcagttcga
    26101 cataaagatc aaattccggc agataaaaat cgggcgtgaa cgactcgacg accctgccct
    26161 gctcgtccca ttgaagggga aaagaccgcg gctcatactc ccaggggatc cggtagaaat
    26221 ccagaagatt cgcgaaaacc tcttcgctgg ggtgagcgaa gcggcgggct ggcaaggagg
    26281 cgttgagccg gagccccgaa agacccatgc gcgccagccg cagccggacc tcaaattgaa
    26341 gctggcgctc ggctgccggc gacagcaagc cggcgtcttc gatgcgcgcg gcgcgggccg
    26401 ccgccgtaat aatctcggcc agccctgcgc tgtcccagcg ctcggcgttc accgacacat
    26461 cgaagcggcc tcgcgaccgg ccgaatttcc ggaaccgggc gcgcagccac gcggcatttt
    26521 cgcggtccag ttgctgtaac gcggccagcg ccgcgggccg ctcgagatgc tgcgccacca
    26581 tcagcattcc agcgcgcgcc gccgggctgg ccgtgatctg aatcctgagc aaaccgggaa
    26641 aatgaggaaa gagaaattcc ccgccccgcc ctgcgaacac cagatgatgt tgggcggcga
    26701 gctgcgcgac aatcgaaatg actagatcgg cgtgcgctcg cggcgggatc ccccccggcc
    26761 ccgcaagctc cgacttgtag agcgcttcca ctcgcgattc cgtaaccaat tcgaagccga
    26821 ggtgggcagc tacctgttgc gcgacctcat ccgcccgcag gccgggctgg gctgagatcg
    26881 tcagaatcgc cataaaccgc agattaccat gcggaaagcg gaccgccgaa agccggcctt
    26941 ccgcagccgg cagccctcgg cggcaagctg tcagcggtcc gctattgata gccgtcagcg
    27001 attggcagag ctcagccgga gcgcatgccg ggaagacaca aaagccaaat gccgggtgcg
    27061 aaagcagaaa actgagggca gaatacccgc cgcccactgc ccgctggttt ccggtggaat
    27121 cctcatgata acgtaaaccg ggatgagtgt agtgatcggt ctggatatcg gcggcacgaa
    27181 gctcgctgga ggcgtgttca gcgaagacgc tgttctgcgc gagcggcgag agcggccgac
    27241 gcgcgcggag cagggcgttg ccgtctcgct cgaagaagtc tatggcttgt tggcggaact
    27301 ggttgccgcg gccgcaaaac acggaccgct ggccggcgtg ggaatttgtt cgcccggccc
    27361 tctcgatccg aaaacagggg tcgtgttcaa tcccacgaac atgcccggct gggtgaacat
    27421 tcctctcgcg gctgagatcg agcgccgctc cggtgttccc tgtcgcgttg agaacgacgc
    27481 caacggggcc ggattggcag aggcgaaatt tggagcggcg cggggcttct cgaaagtttt
    27541 ttacgccacg gtaagcaccg gcatcggcac cggaattatt tttgacggcc gcgtctacca
    27601 cggaaaaaac ggcgccgcag gcgagggagg ccacgtttcc atcgactacc gcagcccggt
    27661 gagatgcaat tgcggtaact ttggatgcat cgaggcgttg gcttcgggca ccgccatcgc
    27721 ccgccgcgcg cgggagaaaa tcgcggcgca tccggagtca aagatgctcg cgctcgccgg
    27781 caaccccggc cagatcagcg ccgcgattgt tgcgcgggcg gcttgccttg gagacaagct
    27841 ggcgattcag ttgatcgagg
//
LOCUS       DASREK010000026         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1830270, whole genome shotgun sequence.
ACCESSION   DASREK010000026 DASREK010000000
VERSION     DASREK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830270"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            465..641
                     /locus_tag="VFA54_01835"
                     /pseudo
     CDS             465..641
                     /locus_tag="VFA54_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            758..928
                     /locus_tag="VFA54_01840"
                     /pseudo
     CDS             758..928
                     /locus_tag="VFA54_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
     gene            complement(1183..3285)
                     /locus_tag="VFA54_01845"
     CDS             complement(1183..3285)
                     /locus_tag="VFA54_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS49574.1"
                     /translation="MRWFVAAFLFCPLAAAAQQIGQNAQPGASGAITFSAGAQLVVET
                     VAVTDEQGKPIEGLTAKDFTVTEDGVPQEIRVFEYQKLPETAAGAPAAGSDSEHIRIY
                     EKLGRMQISPEGAGDARYKNRRLLALYFDMTAMPPADQLRALAAAQKFVRKQMTSADL
                     VAILRFAGGSVDVLQDFTDDRARLLSILQTMIVGEGQGFGESANDASSPDTGAAFGQD
                     DAEFNIFNTDRQLSALQTAANMLGRLSEKKSLIYFAGGLRLTGLNNQAQLRATINAAI
                     RAGVSLWPIDARGLTAQAPLGDATQGSPGNVGMYSGAGALAAAAGFQQSQDTLYALAA
                     DTGGKALFDSNDLAEGILQAQRSVASYYVLGYYPANTAEDGKFRRIRISVNTNVPAHL
                     DYRQGYFAPKKFGKFTAAEKERQLEDALLLADPITELTIAMEIDYFQLNRAEYFVPVT
                     VKIPGRELALAKRSGAERTTIDFIGEIKDAYGTTMTNVRDKVSLKLSGATASELSKRP
                     IVYDAGFTLLPGEYTIKFLARDDETGRIGTYQSTFTVPNLNKEEKRLPISSVVLSSQR
                     TGLKDALYNAGKDKDRRDDANPLVQNGQKLVPSVTRVFRKDQNLYVYLQAYQQGASPI
                     QPLAAVVSLYRGQEKAFETQPIEAAQASGGRLKTIPLRFAVPLAELAPGEYKCQVTVL
                     NAAGQKAAFWQAPILVVP"
BASE COUNT          633 a          994 c         1022 g          668 t
ORIGIN      
        1 tggacagcat agtgatttgt cacgcgcaac tcggtccttc tcggggggta acctggtttc
       61 agaaaggccg ggatcgaaga actatgtcca gcgttttcaa caacgggaat ccccgtcgtc
      121 agctaatttg gacagcagtg actttaacta tattggacac ccggagccgc cacactgtga
      181 cagcgatgag gtgtcaacct cccgacggag catgaagcta agccggcgaa gtttccgtcc
      241 gctgatcggc ggcggtacat ttttgttcag cccaacgtat ctaagagcgc aaggtatggc
      301 tgcgcgcggc gtgaagcccg cgccgcgcgg tctgttcaag gggcacttca cagaagacac
      361 gaacgggctc taccggaacg gcggcagggg caacttcagc gacgtgacgc tcagcgcggg
      421 gctgggcgtc gaaaccagat tcacaggctg ggggggcggg catcgtggat ctcgacaaag
      481 acggctgccc ggagctgttt ttggcgaccg gcaatgttta tccggaactt aggttgccgg
      541 cctatctcta ccggacgccg cggctgctat ttcgcagcct ggggaacggt aaattcgaag
      601 aactgcttca ggaggccgga ccgggcgtcg aagcttccca ttccagccgt ggctgcgcgt
      661 tcggggattt cgataatgac ggcgacctgg atattctgat tattaacctg aacgagcctc
      721 cttcgctgct gcgcaacgat gtgcggggag agaaccggtg gctgaaggtc aagctcatcg
      781 gcgcgcaatc gaaccgcagc gcgattggcg cgcgggtgac ggtagcgtac ggggaaaacg
      841 gcaggcgcag gcggtcgtca gccaggcgag ttacctctcc tgtagcgacc ggcgcctgca
      901 cttcggtctg ggagcaacgg aaacggcgga tatcgaagtc tgctggccga atggcaagaa
      961 acagatcttc tcccaaatcg gagctaaccc aactcgtggt gatccgcgaa ggcgccaaga
     1021 cggtggagcc tgctccgtgg ccgggccgct gagtcgtcct acgatgggag tcagggcaga
     1081 cgggggtctc gatagaagca tgccccgtcc ctttcacgac gcggtagcgc cggccagagc
     1141 gcctgcgctt ccttcagcta agatcgcctg cgactccagc gactacggaa ctaccagtat
     1201 cggggcctgc caaaacgccg ccttctgtcc tgccgcgttg agcacggtca cctggcattt
     1261 gtattccccg ggggcaagct ccgccagcgg aacagcaaaa cggagaggga tggttttcag
     1321 acggccgcct gaagcctgcg ccgcctcgat cggctgcgtt tcgaaagctt tctcttgccc
     1381 gcggtagaga ctcaccaccg ccgccagcgg ctgaatcgga gaagcccctt gctgataggc
     1441 ctggaggtag acgtacaggt tctggtcctt ccggaacacg cgtgttacgc tgggaaccag
     1501 tttctgcccg ttctgaacca gcgggttggc gtcgtcccgc cggtctttat cttttcccgc
     1561 gttatatagc gcatccttca agccggtgcg ctgactgcta agcaccaccg agctgatcgg
     1621 caggcgcttc tcctccttgt tgagattggg aacggtgaac gtgctctggt acgtcccgat
     1681 tcggccggtt tcgtcatcgc gcgccagaaa cttgatggtg tactcgcccg gaagaagagt
     1741 aaagccagcg tcatatacaa tcggccgttt cgaaagctcg ctcgcggttg cgccgctcag
     1801 cttgagactc accttgtcgc gcacgttcgt catcgtcgtg ccgtaggcgt ccttaatctc
     1861 gccgatgaaa tcgattgtcg tgcgctcggc gccgcttcgc ttggccaaag ccagttcgcg
     1921 tccgggaatc ttcaccgtga ccgggacgaa gtattcggcg cggttcaact gaaagtaatc
     1981 gatctccata gcgatggtga gctccgtaat cggatcagcc agcagaagcg catcttccag
     2041 ttgccgctcc ttctcggcgg ccgtgaattt cccgaacttt ttgggcgcaa aatagccctg
     2101 gcgataatcg aggtgcgccg gcacgttcgt attcaccgag attctgatcc ggcggaattt
     2161 gccatcctcg gccgtgttcg ccgggtagta tccgaggacg tagtagctgg ccacggatcg
     2221 ctgcgcctga aggatccctt ccgccaggtc attgctgtcg aacaaagcct tcccgccggt
     2281 gtcggcggcc agggcgtaga gcgtgtcctg cgattgctga aagccggctg ccgcggccag
     2341 tgcgcccgcg ccggaataca ttccgacgtt tccgggcgag ccttgcgtgg catcgcctag
     2401 aggcgcctgt gccgtcaaac cgcgggcgtc aatcggccaa agcgacacgc cggcgcgaat
     2461 ggcggcattg atcgtggccc gcagttgcgc ctggttgttc aagccagtca gccgaagacc
     2521 tccggcaaag tagatcagcg acttcttttc gctaagccgg cccagcatgt tcgcggcggt
     2581 ctggagggcc gatagctggc gatcggtatt aaaaatattg aactccgcat cgtcctgacc
     2641 aaaggctgcg ccggtatccg gagagctggc gtcattcgca gattcgccaa agccttggcc
     2701 ctcgccgacg atcatcgttt gcagaatgct cagcaggcgg gcgcggtcat cggtgaagtc
     2761 ctggagcaca tccacggatc ccccggcgaa acgtaggatc gcgacgagat cggcggatgt
     2821 catctgcttt cgcacgaatt tctgcgccgc ggctagcgcg cgcaactgat cggcaggagg
     2881 catcgccgtc atgtcgaaat agagggcgag cagccgccgg tttttgtatc gcgcgtctcc
     2941 ggccccttcg ggcgagatct gcatgcggcc gagcttctcg taaatgcgga tatgctcgga
     3001 atcggatccc gccgcagggg ccccggcggc cgtttccggc agcttttgat actcgaagac
     3061 gcggatttct tgcggcacgc cgtcttccgt taccgtgaaa tccttcgcgg tcaagccctc
     3121 aatcggtttc ccttgctcgt ccgttactgc cacggtttca acaaccagtt gcgcgccggc
     3181 ggagaaagtg atcgccccgg atgccccagg ctgcgcattc tgcccgatct gctgcgctgc
     3241 ggcggccagc ggacaaaaca ggaacgcggc gacgaaccat cgcatgcgtc agaacctcaa
     3301 gcgcagcgaa gtttgca
//
LOCUS       DASREK010000027         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2159042, whole genome shotgun sequence.
ACCESSION   DASREK010000027 DASREK010000000
VERSION     DASREK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159042"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1876
                     /locus_tag="VFA54_01850"
     CDS             107..1876
                     /locus_tag="VFA54_01850"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZS49575.1"
                     /translation="MKPEPHEAGRQSGLRSGLTASPAAAACLFAVITAAVLPAANADL
                     ILYNGKIVTVDEKFSLAEALSVKNGKITKVGTTRQVFSTERGPRTLRIDLNGRTVLPG
                     LIDSHVHPIEAGLSELQRPLPPLDSFEAIQNFIRDKMASTPKGRWIIVPRTFPTRLRE
                     MRMPTREVLDVAAEHPVLFDASYTVVVNSFALKISGITRDTPNPPNGEIVKDARGEPN
                     GILRNAQSLLKGLNRGDSATDQEKLAGLEKMLKLYVEAGLTAVTDRALTPAEFPLYEK
                     LKTEQRLPLRVVMTWWIDASRPTEELVRQIRTAPYVTGKGDSWLKFGALKVTLDGGMT
                     IGTAFQRTPYGAFGKQLYGLTRPDDRGQLFIDREKLRAIERAGHARGWQLAAHDQGGG
                     AIDAMLDAFEAADKDRPIAASRSHLIHASFQSPEAIARAKRLGILIDAQSDWLYRDAE
                     ALEKVFTSEGMRYFYPMRSYLDAGLIVAGGSDHMIGYDKNRAVNPYNPFLQMWIAVTR
                     QTDRGRVIHPEQRISREEALKTHTIWAAYMQFAEQERGSIEPGKLADLVVIDRDYLTC
                     PEDQIKSIEPQMVIVDGRIAMQR"
     gene            complement(1929..>2629)
                     /locus_tag="VFA54_01855"
     CDS             complement(1929..>2629)
                     /locus_tag="VFA54_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49576.1"
                     /translation="VLFRPATMLFAAGAVLVGFFVWYLSARTGGPDLPPPTVSYTLLP
                     NEGKTKGVPVAIKVNQLTVFLIDDPMEDGGAGRSKEVVANLNEVIQPLKAGAEVRFAV
                     QTVNGRPAIVEVSQEGVQPKTLVSVTEGDEALAGETDANRVAAQWAERLTDAVKVFVF
                     GEAPTFSTGTEFGDSLLAMYKAAAAETRGKVSKKALDQAYQRLPAAQREALEAPPLAR
                     KQHAGRPSALRKAG"
BASE COUNT          533 a          815 c          792 g          489 t
ORIGIN      
        1 ctaagtcctg gaagcagcgc ggcatgaaat tcattggctg cggcagcgaa atttcgttca
       61 tgttggagaa ggctcgggag gtggtcgccc aattacggga gggcgaatga aaccggaacc
      121 gcacgaggcg ggacggcaat ccggactgcg cagcggtctg acggcttctc ctgccgccgc
      181 agcctgcctt tttgccgtca tcaccgccgc cgttctccca gcggctaacg ccgacctgat
      241 tctctacaac ggcaagatcg tcacggtaga cgaaaagttc tccctggcgg aagcgctttc
      301 ggtcaaaaac ggcaagatca ccaaggtggg cacaacccgg caggtttttt cgaccgagcg
      361 cggcccgcga accctgcgta tcgacctcaa tggccgcacg gtgctgccgg ggctgatcga
      421 tagccatgtt cacccgatcg aagccggtct cagtgagctg caacgccctc tgccgccgct
      481 cgattccttc gaggcgatcc agaatttcat ccgggacaag atggcgtcga cgcccaaggg
      541 ccgctggatt attgttcccc gcactttccc cacgcggttg agagaaatgc gcatgcccac
      601 gcgcgaagtg ctcgacgttg cggccgaaca tcctgtgctg ttcgacgcca gctatacggt
      661 ggtggtgaat tccttcgctt tgaaaatcag cgggatcacc cgggatacgc cgaatccgcc
      721 gaatggcgaa atcgtcaaag atgcccgggg cgagccgaat gggattttgc gcaacgcgca
      781 gtccctgctg aaaggcctga accgcggcga ttcggccaca gaccaggaaa agctggccgg
      841 ccttgagaaa atgctgaagc tgtacgtgga agccgggctg actgccgtta cggaccgcgc
      901 cctcactccc gccgagttcc ctctttacga aaaactgaaa accgagcagc ggctgccctt
      961 gcgcgtggtc atgacgtggt ggatcgatgc ctcgcgcccc accgaagagc tggtccggca
     1021 gatccggact gcgccctacg tgactggcaa aggcgattcg tggctgaaat ttggagcgct
     1081 taaggtcacc cttgacggcg gcatgactat cggcacggcc ttccagcgca ctccttatgg
     1141 ggctttcgga aagcaactct atgggctgac gcggcctgat gaccgcggcc agctcttcat
     1201 agatcgcgaa aagctgcgcg ccatcgagcg ggccggccac gcgcgcggct ggcagctcgc
     1261 ggcgcatgat caaggcggcg gcgccattga cgccatgctg gatgcattcg aagcggccga
     1321 taaagatcgc cccattgccg ccagccggtc gcatctcata cacgccagct ttcagagccc
     1381 ggaggcaatc gctcgcgcca agaggctggg catcttaatc gacgcccaat ccgactggct
     1441 ctatcgcgac gccgaggccc tggagaaggt ttttaccagc gaaggaatgc gctatttcta
     1501 cccgatgcgc tcctatctcg atgccgggct gatcgtcgcc ggaggcagtg atcacatgat
     1561 cggatacgac aaaaacaggg cggtcaaccc gtataatccc tttctacaaa tgtggattgc
     1621 cgtcacgcgc cagacggatc gcggaagggt tatccaccct gagcagcgca tcagccggga
     1681 ggaggctctg aagacccaca ccatttgggc ggcttacatg caattcgctg aacaggaacg
     1741 agggtcgatc gaacccggca agctcgccga cctggtggtg atcgaccgcg attatctcac
     1801 ctgtccggaa gaccagatca aatccatcga gccgcaaatg gtgatcgtcg atggcagaat
     1861 cgccatgcag cggtaggcga ttaggaatgc gcggctgttg ccggcgggcg gtttttgcgt
     1921 gccgcctgtt atccggcctt gcgcaaggca gaaggtctcc cagcgtgctg ctttcgggcc
     1981 aggggagggg cttcgagcgc ctcacgttgc gctgcgggaa gacgctggta ggcctgatcg
     2041 agcgctttct tgctgacttt gccgcgggtc tcggcggccg cagccttata catcgccagc
     2101 agcgagtcgc caaactcggt tccggtagag aaggtaggag cttcgccaaa cacgaaaacc
     2161 ttgaccgcat cggtgagccg ttcggcccac tgagccgcca cgcggttggc atcggtttct
     2221 cctgccagag cttcatcgcc ctccgtcacc gaaaccagag tctttggctg gacgccttcc
     2281 tggctgacct cgacgatcgc cgggcggcca tttaccgtct ggacggcgaa ccgcacttcc
     2341 gcgcccgcct tcaacggctg gataacctcg ttgagattcg ccactacttc cttggaacgg
     2401 cccgcgcccc cgtcttccat cgggtcatcg atcagaaaga cagtcaactg attgaccttg
     2461 atcgcgaccg gaaccccttt cgtcttcccc tcgttcggaa gcagcgtgta agagactgtg
     2521 ggcggaggca gatcggggcc gccggtgcgg gcgctcaaat accagacaaa gaatccgacc
     2581 agaaccgcgc cggcggcaaa aagcattgtc gccggccgga agaggaccg
//
LOCUS       DASREK010000028         4660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1992821, whole genome shotgun sequence.
ACCESSION   DASREK010000028 DASREK010000000
VERSION     DASREK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992821"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1130)
                     /locus_tag="VFA54_01860"
     CDS             complement(312..1130)
                     /locus_tag="VFA54_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZS49577.1"
                     /translation="MATPDFAPRRAAFRKLHESGCFVIPNPWDIGSARYLKHLGFQAL
                     ATTSGGFAFSRGLADTQVSREVVLDHIAEIVAATDLPVNADFESGFARDPKGVAESVR
                     LCAATGVAGISIEDSTGDKAKPLYEVPVAVERLEAAREALGDSGVLLIGRAECFLTGH
                     SEPLKESIRRLQAYAQAGADVLYAPGVHERADIQAIVAAVHPRPVNVLMGWSSGDKVT
                     DLAALGVRRISTGSALARAAWGGFISAAKKIAEQGSFAFDGAAPFAELNSLFRA"
     gene            1184..1426
                     /locus_tag="VFA54_01865"
     CDS             1184..1426
                     /locus_tag="VFA54_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZS49578.1"
                     /translation="MPLYDFKCLDCGEVFEALVLKEQPACPACQGRKLEQQISLFAVD
                     SESIRQNNINSARKANSKMARDKAIADREAILHHDD"
     gene            1442..4165
                     /locus_tag="VFA54_01870"
     CDS             1442..4165
                     /locus_tag="VFA54_01870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZS49579.1"
                     /translation="MTCRTCSQEVPDASRFCPSCGAELNGGRFGAGPATQTVAAAASP
                     HPVLTPSRSGLSSSSGSLPEGRFLPGALLANRYRIVALLGRGGMGEVYRAHDLTLGQE
                     VALKFLPEAAAKNPGALARFYNEVRIARQVSHPSVCRVYDLGEVDGQPYLSMEYVDGE
                     DLGLLLRRIGRLPSDKAVEIARQLCAGLAAAHAKGVLHRDLKPANVMLNGRGEAVITD
                     FGLAALADEIPGDEIRNGTPAYMAPEQLAGKEVTVQSDIYSLGLVLYEVFTGKRAFEA
                     STLAELVRLHSETTPVSPSSLVKELDPAVESAILRCLDAEPANRPHSALAVAARLPGG
                     DPLAAALAAGETPSPQMVAASGDTAALPWKIAVPSLAAALAGLVAFVFVGASSSMIEK
                     EQLTKSPEVLADKARELIQNLGYTAMPADSAGAFSDNTDFIDWVQKTDGPYPQWDEIA
                     RSRPSPLGFWYRQSLEPMKPAGMHNQMLIPGGIAEDDPKPTESGMINVRLDPQGRLTF
                     LEALPAQKEEPPTKIAPPDWNALFAAAGLDISKFQPAPPVWASLAASDTRQAWNGQWP
                     GYKNRPLHVEAAAWHGRPVYFSVTGPWTVPARMPSNSPENLSNLTRRFLLIGFILVPV
                     GLAVLLARWNWVRGKGDRRGAMHLASLIFILHMLLWAFQTHVASAGDFLYLLFVSIAT
                     SLLWGGAVWILYLALEPYVRRYWPQAIVSWTRALSGRWRDPLVGRDVLYGAILGVLIC
                     DLYGARNYLQGRLGAPPAFFSTEYLASARTAIGAWFTQFPGSISATLLVFMLLFVFRV
                     LLRKAWLGAIGFVLFWTTLQAVSSNYPAVEWPMQALVYTAVAAAAIRYGLVAQVVGLF
                     VADMALNVPVTLNPSEWYFPAAMLAMASVAGLAVWGFYTSLAGETPWKTRT"
     gene            complement(4162..>4660)
                     /locus_tag="VFA54_01875"
     CDS             complement(4162..>4660)
                     /locus_tag="VFA54_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS49580.1"
                     /translation="KRGDFLQLSDEDWADGFALKFFGAMRLTRAAWPHLKAQAGSVMN
                     ISGVGGRMPGPLFTIGGSVNAALLSFTKAMADVGIRDGVQVNAVNPGSIRTERYRRML
                     EATAADQGVDVETAERRMIESARTTRIGEPGDIASLVAFIVSPEGRFLQGSLIDMDGG
                     ATKVI"
BASE COUNT          847 a         1395 c         1487 g          931 t
ORIGIN      
        1 cgccggagga gtcaaacgcc ggctgagcca aagaggaaaa ggcggacgca cccgcgagca
       61 gcactgcaaa aagagtaggt ttcatttgga ccggaacacg aagccgctta aaggcctctc
      121 aacagcttga cgcgcgaaag atcaacatgg ttaacgacac cggcgagttt cctgccggct
      181 cgaagggtgg cgccgccgga cttacttgat gacgccttat tgattacagc acgacttagc
      241 cgcgaaagaa ggccggttgc gatgcgtaaa gttttgtaca cctataacct gggaacttcc
      301 ctcgcttaac gtcaggccct aaacaaacta ttgagttcgg cgaatggcgc tgcgccatca
      361 aaagcgaagc ttccctgctc ggcaattttt ttggccgcac tgatgaatcc accccacgcg
      421 gcgcgtgcca gcgcactgcc ggtactgatg cggcgcacgc ccaaagcggc cagatcggtg
      481 actttatcac cgctgctcca gcccatcagc acgttcaccg gcctggggtg tacggccgcg
      541 acgatggctt ggatgtcggc gcgttcgtga acgcctgggg cgtagaggac gtcggcgccg
      601 gcttgcgcgt acgcctgcag ccgccggatg gattccttca gcggttcgga atggccggtc
      661 aggaagcatt cggctcggcc gatgaggagg acgccggaat cgcccagggc ttcgcgcgcc
      721 gcctcaagcc gctctaccgc caccgggacc tcgtacaaag gcttcgcttt gtcgcctgtg
      781 gaatcttcga tcgaaatgcc ggccacgcct gttgcggcgc acaggcgaac actttcggcg
      841 acacctttgg ggtcgcgcgc gaagccggat tcaaaatcgg cattgacggg cagatccgtg
      901 gcggcgacaa tctcggcgat gtggtccagc acgacctcgc gcgagacctg cgtatccgcc
      961 aacccgcgcg aaaaagcgaa accaccgctg gtagtggcca gcgcctggaa accgagatgt
     1021 ttgagatagc gcgccgatcc aatatcccag gggttcggaa tcacaaaaca tccgctctca
     1081 tggagcttcc ggaaggcggc gcggcgtggt gcgaaatcgg gggtggccat atttgctgag
     1141 gactagaagc tagcataatg gatattgccg aaaggagatt cgcgtgccgt tgtacgattt
     1201 taaatgcctg gattgcggcg aggtattcga ggcgctagtt ctgaaggaac agccggcgtg
     1261 tcccgcttgc caggggcgca agctggagca gcagatctcg cttttcgccg tcgactcgga
     1321 gagcatccgc cagaacaaca ttaattccgc gcgcaaagcg aattccaaaa tggcgcgcga
     1381 taaggcgatc gcggaccggg aagcgattct gcatcacgac gactgataag agcaagcctc
     1441 cttgacctgc cgaacctgtt cgcaagaggt cccggacgcg agccgcttct gcccttcgtg
     1501 cggcgcggag ttgaacggag ggcgctttgg cgccgggccg gcgacacaaa cggtggctgc
     1561 cgccgccagt ccccatcccg tccttacgcc gtcgcggtcg ggtctttcat cctcgtccgg
     1621 ttcccttcct gaagggcggt ttcttccggg cgcgttgctt gcgaatcgct accgcatcgt
     1681 cgcactactg ggccggggtg gaatgggcga agtgtatcgc gcgcacgacc tcaccttggg
     1741 ccaggaggtg gcgcttaagt tcctgccgga agcggcggcg aaaaatcccg gagcgctggc
     1801 gcggttctat aacgaagtcc gtatagcgcg tcaggtctcg catccgagtg tctgccgcgt
     1861 ttacgatctg ggcgaagtgg acggacagcc gtatctttcc atggagtacg tggatggcga
     1921 ggatctggga ttgctgctgc ggcgcatcgg gcggctgcct tcagacaaag ccgttgagat
     1981 cgcgcggcaa ctatgcgcgg gcctggcagc cgcgcacgcc aagggcgtac tgcatcgcga
     2041 tctgaagccg gccaacgtga tgttgaacgg ccgcggcgaa gcggtcatca ccgattttgg
     2101 gctcgcggcc ctggccgatg aaatcccggg agacgagatc cgcaacggca cgcccgccta
     2161 catggcgcct gagcaattgg cgggaaaaga agtaacggtg cagagcgata tttattccct
     2221 cggcctggtg ctgtacgaag ttttcacggg caagcgcgcc ttcgaagctt caaccctcgc
     2281 cgaactggtg cggctccata gcgagacgac gcctgtgagt ccgtcgtcgc tggtcaagga
     2341 gctcgatccg gccgtagaga gcgcgatact acgctgtttg gacgcggagc cggcgaatcg
     2401 tccccattcg gcgctggcgg tggccgcgcg attgcccggg ggcgatccgt tggctgcggc
     2461 gctggcggcc ggtgagacgc cttcgcccca gatggtggct gcttcgggcg acacggcggc
     2521 tttaccgtgg aagatcgctg tcccgagcct ggccgccgcg ttggcgggcc tggtggcgtt
     2581 cgttttcgtg ggtgcctcgt ccagcatgat cgagaaggaa caattgacga aatcgcctga
     2641 ggtgctggcg gacaaggccc gcgaattgat ccagaacctg ggctacactg cgatgccggc
     2701 ggattcggcc ggtgccttca gtgacaatac ggattttatc gattgggtgc agaaaaccga
     2761 cggcccgtat ccgcagtggg atgaaatcgc gcgcagccgt ccgtcgcctc tgggattttg
     2821 gtatcgccag agccttgagc cgatgaaacc ggccggaatg cacaaccaga tgctgatccc
     2881 cggcggaatc gcagaggatg atcccaagcc caccgagtcc ggcatgatca atgtccggct
     2941 ggatccgcaa gggcggctaa cttttttgga agcgctgccg gcgcagaaag aagagccgcc
     3001 caccaagatc gcgccgcccg attggaatgc gttgttcgct gctgccgggc ttgacatctc
     3061 gaaattccag cccgcgccgc cggtgtgggc ctcgctcgcg gcttcggata cgcggcaggc
     3121 ctggaacgga cagtggcccg gatacaagaa tcgcccgctc catgttgaag ccgccgcgtg
     3181 gcatggaagg ccggtctatt tttcggtgac agggccctgg accgttccag cccggatgcc
     3241 atccaactca ccggagaacc tcagcaacct gacacgccga ttcctgctca ttggcttcat
     3301 cctggtgcca gtaggactag cggttcttct ggcccgctgg aattgggtac gcggcaaagg
     3361 cgaccggcgg ggcgcgatgc atctggcatc gctgatcttc attttgcaca tgctgctgtg
     3421 ggccttccaa actcacgtcg cctcggctgg agatttcctc tatcttttat tcgtgtcgat
     3481 cgcgacgtcc ctgctttggg gaggcgcagt gtggatactg tatctggcgc tggagccgta
     3541 tgttcgccgt tattggccgc aggccattgt ttcctggacc cgcgcgctga gcgggcgctg
     3601 gcgggatccc ctggtggggc gagacgtgct ctacggagcg attttgggcg tgttgatttg
     3661 cgatctttac ggcgctcgta attatctgca aggccgactg ggggcgccgc cggcgttctt
     3721 ctccaccgag tatctcgcga gcgcgcgcac tgcgattggc gcctggttca cccagtttcc
     3781 ggggtctatc agcgccactc tgctggtctt catgcttctc tttgtcttcc gggtgctgct
     3841 ccgaaaagca tggctcgggg cgattggatt tgtgctgttc tggactactc tccaggctgt
     3901 gtcttccaac tatccggccg tggagtggcc gatgcaggcg cttgtctata ctgctgtcgc
     3961 tgcggccgcc atacgttacg gactggttgc acaggtggtg ggcctgttcg tagcggacat
     4021 ggcgttgaat gttccggtga cgctgaatcc atccgagtgg tatttccccg ccgcgatgtt
     4081 ggcgatggcc tccgttgccg ggcttgcggt ctggggattt tatacatccc tggcgggcga
     4141 aacgccctgg aagacccgaa cctaaatcac tttcgtcgcg ccgccgtcca tatcgatcag
     4201 ggaaccttgt aagaagcgcc cttccggact cacgatgaac gcgaccaaac tcgcgatatc
     4261 gccgggctcg ccgatgcgcg tggtgcgcgc gctctcgatc atgcgccgct cggcggtctc
     4321 gacgtcgaca ccttgatccg cggccgtggc ctccagcatg cgccggtagc gttccgtacg
     4381 aatgctgccg ggattgacgg cgtttacctg cacgccgtcg cgaatgccga cgtcggccat
     4441 ggcttttgtg aacgaaagga gcgccgcatt cacggacccg ccgatggtga aaagcggccc
     4501 gggcatgcgc ccgccgacgc ctgaaatatt catcaccgat ccggcttgcg ccttcagatg
     4561 cggccacgcg gcgcgcgtca ggcgcatggc gccgaagaat ttgagcgcga agccgtcggc
     4621 ccagtcttcg tcgctgagtt gcaggaaatc gccgcgcttg
//
LOCUS       DASREK010000029         9018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1832208, whole genome shotgun sequence.
ACCESSION   DASREK010000029 DASREK010000000
VERSION     DASREK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832208"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VFA54_01880"
     CDS             <1..1607
                     /locus_tag="VFA54_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49581.1"
                     /translation="QTQGPTGGGSGFASYLLGDVANFARYVSNVYDASERQNRLFFYA
                     QDNWRVTSKLTLNYGLRWDIYRPEYVNGPGKGANIDPATGENLVAGSQGVSNSLNTSI
                     PPRTFAPRAGLAYKITDKTVIRAGYGRGFDLGIFGSIFGHNVTQNLPVLAIQNVTPAF
                     NYQSVFTLTQGPPPVSDPAAILAAQPKGPNGRPLQPNGFTTDIVPLTQRVPTIDTWNF
                     TLQRQLTGTASLEVAYVGTKGTHEPPAYNGYSNTNEPTLVGYTAGLSTNQRRPYYGLY
                     GWTQLVRYSGNDSSNRYHALQTKLEKRFSRGLQLLGHYTWSRALDYDTTQYIYRRDLG
                     FGPNNANRNHIITAMGLWEVPVGNGRKALNHLPKALDSVIGGWQTNFVTTWMTGLPFT
                     PSYQNCSADADTGFCRPNVVGPWQTSDPSQFGWFSTAAALLSANGQISGPWQRPDRGQ
                     FGNVGRNTLTGPRFWQTDFSAFKQFQLKERMKLQFRAEAFNFFNHTNLGQPAACVDCP
                     GTAGRIFATATTYLPRMWQVAARIQF"
     gene            1823..3616
                     /locus_tag="VFA54_01885"
     CDS             1823..3616
                     /locus_tag="VFA54_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49582.1"
                     /translation="MRVTRRRFVGSAAGLAGFAGAGRPLMAQARKPANRQQATENPYL
                     GLVKANDEAAAALISESGRNEGRGRIRQVGEALETLAAAFCAPESRFYRADILLSPME
                     QLSAALRKAQHADGTIDSGNLHSPPDTGFVVETVATALAVLRRNGDPRLSKSEENMAA
                     FLRGAGEALVTGGVHTANHRWVVCSALARINALLPAAKYVDRIDDWLGEGIDIDADGQ
                     YSERSAGIYSRVTDNALITVARLLDRPGLLDPVRRNLDLNVYLMHPDGEIETVGSRRQ
                     DQFMIGSISDYYLAYRYLAIRDDNALYAAITGFIEQQRLASLERTNRAIHFLEEPLLR
                     EALPAGGVIPSDYAKVFPNSGLARIRRGDVSATVYGGSDWPLGVASGLASNPTFFTFR
                     KSKAILQSVRMGAAFFSLGAFHSAGLEANGNRYSLHQRFHVPYYQPLPKAQRSARGDY
                     PLTPAADKRFWNKMNFPNRPPSNVQTLEQRVTITENHGVCELNFDISGQAGVPVTIEL
                     SFRTGGKLSGSLQELPREKCFLLKQGFGNYRVGDDAIEFGPGQAEHQFLNLSGHSYIA
                     HGGALKAGGECVYITGFTPFQKVITIRGASA"
     gene            4018..4107
                     /locus_tag="VFA54_01890"
     tRNA            4018..4107
                     /locus_tag="VFA54_01890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4052..4054,aa:Ser,seq:cga)
     gene            complement(5385..6266)
                     /locus_tag="VFA54_01895"
     CDS             complement(5385..6266)
                     /locus_tag="VFA54_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZS49583.1"
                     /translation="MAAAEILSWPFDQSFPLEVQPALARDFHAELCRHAYFELVFLEV
                     GEIVFEVEGRLVAMRAGDLFVMGSALLHRVHTYVCEDGRGIALHFLPELIRAGDMTHE
                     DIEYLTPFLVQNEEFSHVVPAATGVPAQVRDLMERIRSELPARSDRNRLVAKTYLKMI
                     LVLLMNYYTATLSSTTFLHRRERDFERLKPLLDFIERHYSERLTLEQAAAILHLSPSH
                     FMRLFRRVTGEPLMSYLNRFRIAKAEALLASTNKTIAQVSQEVGFCDQSYFGYIFRKL
                     VHMTPREYREQRRVNTQ"
     gene            6720..7784
                     /locus_tag="VFA54_01900"
     CDS             6720..7784
                     /locus_tag="VFA54_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS49584.1"
                     /translation="MDNVDRRDFLRGAAGAFAAASALAAPAGNEKVRVGLYGTRYSHL
                     IGKIGIMKRASDTFEIAGAYEPEGTPPDNPAFNGIKIVSEDQLLGDPSIHLIIVEGQV
                     DKTVPWARKAIAANKHVHLERPPGVEMEPFRQLVEEARRRKLLIQVGYPWKFSPGVVA
                     AFEAVQKGYLGDVYMVRATVNTDIDARARAGMQRFKTGIFYDLGPYMVDLVVALLGKP
                     KEVKGWFHHDDSGFPDNIIDNSLAVLEYPKAFAVVSCNARQAGSREQRQFEIIGTDGW
                     ANLQPVEPGRLHLHLREARGPYKAGPQEVALPVPPVPRWTGDLLEMARAIRTGTPLKY
                     SYDHELLLQETVMRITGGRG"
     gene            8177..8380
                     /locus_tag="VFA54_01905"
     CDS             8177..8380
                     /locus_tag="VFA54_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZS49585.1"
                     /translation="MKEKGTVKWFNAAKGFGFIQRDNGDDVFVHFSAIQANGYRSLDE
                     GAQVEFSVKKGPKGLQAEDVAVV"
     gene            8580..8795
                     /locus_tag="VFA54_01910"
     CDS             8580..8795
                     /locus_tag="VFA54_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZS49586.1"
                     /translation="MSGTIKKLIAERNFGFIRAENGQDVFFHASAVQQNGFASLAVGQ
                     AVNFDLERGEKGPKASNVRARREQPLS"
     gene            8818..>9018
                     /locus_tag="VFA54_01915"
     CDS             8818..>9018
                     /locus_tag="VFA54_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49587.1"
                     /translation="MQLQYIGFTQTENTREYIFHGLVHGETTRVFVMSADMALFLKHR
                     VGVQEGPVLCLRTLTAELGAQDQ"
BASE COUNT         1886 a         2610 c         2683 g         1839 t
ORIGIN      
        1 cgcaaacgca ggggccgacc ggcggcggca gcggtttcgc gtcctatctg ctgggcgacg
       61 tggccaactt cgcgcgatat gtcagcaatg tttacgacgc cagcgagcgg cagaatcgtc
      121 ttttcttcta tgcgcaggat aactggcgcg tcacatccaa gctcactctg aattacggct
      181 tgcggtggga tatctaccgg cccgagtacg tcaacggacc cggcaagggc gccaatatcg
      241 atcctgcgac tggcgagaat cttgtggccg gctcgcaggg ggtcagtaac agcctcaaca
      301 cgagcatccc gccgcggact tttgcaccgc gggccggttt ggcgtacaag atcaccgata
      361 agacggtgat tcgtgcgggc tatggccgtg gattcgacct tggcatattc gggtccatct
      421 tcggccataa cgtgacgcaa aacctccctg tattggcgat tcagaatgta accccggcgt
      481 ttaactatca gagcgtgttc actttgaccc agggcccgcc gccggtgtcc gaccctgccg
      541 ccattctcgc tgcgcagccg aaagggccaa acggccgccc gctgcagccg aatggattca
      601 caaccgacat tgttcctctt actcagcgag taccgactat cgatacctgg aatttcacat
      661 tgcagcggca gttgaccggc acggcgtctc tggaagtggc ttacgtgggc acaaagggaa
      721 ctcacgagcc gccggcatac aacggatact ccaatacgaa cgagccgacg ctggtcggct
      781 atacggcggg tctatcgacc aaccagcgcc ggccctatta cggtctctat ggatggaccc
      841 agcttgtgcg ctattccggc aatgactcca gcaaccgcta tcacgccctc cagaccaagc
      901 tcgagaagcg attttcccga ggcttgcagt tgctgggtca ctacacctgg tcccgagcct
      961 tggattatga cacaacccaa tacatctaca ggcgagatct gggctttggt cccaacaatg
     1021 cgaaccgaaa ccacattatt accgccatgg gattgtggga agttcccgtc gggaacggcc
     1081 gtaaagcgct caatcacctt cccaaggcgt tggattccgt aatcggggga tggcaaacaa
     1141 acttcgtgac gacctggatg acggggctgc cgtttacgcc cagttatcag aattgtagcg
     1201 ccgatgcgga tacgggattc tgccggccca acgtggtcgg accatggcaa accagcgatc
     1261 ccagccaatt cggctggttc tcgacagccg ccgcattgtt gtcggctaat ggtcagatca
     1321 gcggaccatg gcagcgcccg gaccggggac aattcggcaa cgtcggccgc aacacgctga
     1381 ccggaccgcg tttttggcaa acggatttct cagcctttaa gcaatttcag ttgaaggagc
     1441 gtatgaagct ccagttccgc gcggaggcgt tcaatttctt caatcacacc aatctcggcc
     1501 agcccgccgc gtgcgtggat tgccccggaa cagccggacg aatctttgcg accgcgacga
     1561 cctacctgcc gcggatgtgg caggtggcgg cgaggattca gttctgacat tggcggcctg
     1621 aactgttgat cggcgaactc gcgagggccg cccgtgggcg ataatcacca caggccttgc
     1681 tgcggcagag gtctggacgg cgaccatctc cctggttccg tggctgaagc ggatccaccg
     1741 cgggtccggg aggaagtcca acgcgccggc gcgtcgattt taaccgtagg cgcaggcgag
     1801 actcattttg gaggggagca tcatgagggt cacgcggagg agatttgttg gcagcgcagc
     1861 cgggctggct ggttttgccg gcgcgggccg gccgctgatg gcgcaggccc gcaagccggc
     1921 caaccgccaa caggcgacgg agaatcctta tctcggtctg gtgaaggcca acgacgaagc
     1981 cgccgccgca ctgatttccg aatccggccg caacgaaggc cgcgggcgca ttcgccaagt
     2041 gggggaagct ctcgaaactc tggcggcggc tttttgcgct ccggagtcgc ggttttaccg
     2101 ggcggatatt ctcctttctc ctatggagca actatcggcc gcgctgcgga aggcgcagca
     2161 cgccgacggc acgatcgatt ccgggaatct ccattcgcct cccgacaccg gctttgtcgt
     2221 ggaaacggtg gccactgcgc tggcggtgtt gcgccgcaac ggagaccccc ggctgtcgaa
     2281 gagtgaggag aacatggctg cttttctccg cggcgccggc gaggctctgg ttactggcgg
     2341 ggtacacacg gccaatcatc ggtgggtggt ctgttccgcc ttggctcgca tcaatgcact
     2401 gctgccggcc gcgaaatacg tggatcgtat tgacgactgg ctgggcgagg gcattgacat
     2461 cgacgccgac ggccaatata gcgagcggag cgcgggaatt tattcgcggg tgaccgacaa
     2521 cgcgctgatc actgtagcgc gcctgttaga tcggcccggt ctgctggatc cagtgcggcg
     2581 aaatctggat ctgaacgttt atctcatgca tccggatggc gagattgaaa cggtcggatc
     2641 ccggcggcag gaccagttta tgatcggctc catatcggat tactaccttg cctaccgcta
     2701 cctggccatt cgagatgaca acgcactcta cgccgctata acgggattta tcgagcagca
     2761 acgtctggcg tcgctggaaa gaacgaaccg cgcgatccat ttcctggagg aaccccttct
     2821 gcgagaggcg cttccagcgg gcggcgttat tccttcggac tatgcaaagg tgtttccgaa
     2881 cagcgggctg gcgcggattc ggagaggaga tgtgagcgct acggtctatg gaggctccga
     2941 ttggccgctc ggggtcgctt ccggcctggc cagcaaccct acctttttca cttttcgcaa
     3001 gagcaaagcg attctgcaat cggtgcggat gggcgcagcg ttcttcagct tgggggcgtt
     3061 ccacagcgcc gggcttgagg ccaacggcaa tcgctattcg ctccaccagc gttttcacgt
     3121 accttattat cagccgctgc cgaaagcgca gcgcagcgcg cgaggcgact atccgctgac
     3181 gcccgcagcc gacaagcgat tctggaacaa aatgaacttt ccaaaccgtc ccccgagcaa
     3241 cgtccagacc ctggaacaaa gagtgacaat cacggagaat cacggcgtgt gcgagttaaa
     3301 tttcgatata tcgggccagg cgggcgtgcc ggtcactatt gagctctcgt tccgcactgg
     3361 cgggaagctt tcgggctcgc tccaggaact gccgagagag aagtgttttc ttctcaaaca
     3421 gggttttggc aattatcgcg tgggagacga cgcgattgag tttggtcccg ggcaagccga
     3481 gcaccagttt ttgaacctct ccgggcactc ctatatagcg catgggggcg cgctcaaggc
     3541 gggcggcgag tgcgtctaca ttacgggctt cacacccttc caaaaagtaa tcacgatacg
     3601 cggcgccagc gcttgatgaa ctcatacggg ctcccggctg gctcgaatag ccggtcgcga
     3661 ggcgggggcg acccaaccct ccgacgcaac agcagccggc cgctgcacgg gccgccgccc
     3721 agcccgcccg cgcacaaggt aggattctgg ttccgacgat gaccatttca gcgtacggcc
     3781 agcggcgcaa atcagcaatg gtagagaagc cggttgtccg cattgcctgt ttgtaccagc
     3841 aaacgaacaa aagcaatgga aaatgtttgc ttctctgaga acgcgcggcc ggcgtccgcg
     3901 cgatctgatc ccggcagatc tgggaccgct cgaggctgac ccttcctgct gattcatgaa
     3961 gcagcggagc ggttcttcag ttgcggatgt agtgtagttc gagtattttg aagatacggt
     4021 gaggtgcgag agcggttgaa tcgggcggtc tcgaaaaccg ttgagccttt acgggctccg
     4081 tgggttcgaa tcccaccctc accgccatac ttactgaaaa taaaggtttt aacatcatct
     4141 aactctcttt ttcttgcccg tatctcttta aaaaatcgtc cgtggtcaac ccatcctcac
     4201 cgcccggaaa tcactcactt cgcaagggcc gcgggcaatg ctgggggttg cgggcaggcg
     4261 gtgctccgat ttcaagccct gatccatgtg ggcggcggcc agcccgaagc gcagcttcat
     4321 acaaacaccg agcgtgcttt cggtttctaa tgatttgggt atagtcggaa cagcggactc
     4381 cggaggggcg tgtggcgcag gcaaagatcg aactcaagat cggtgacttg gctttcagtc
     4441 gtgatgctga tgccgcatgg ctcgaaaagc agctacaagc tactggacct cgccagaagc
     4501 gcgggtgtcg accagaacgt ggaaagtgcc ggtgctgaaa ctgaagtgac ctgcgaacgg
     4561 accagcaact gtgggactct gagtgcattc ctaaaaccca cactgcgata gggaatcagg
     4621 ttcgcaaatt gctcgctact gcgacttggc tgacgctcgg cggacagaca cgactcacta
     4681 cgtcgcaagt gacagagtgc ggtggccccc aactcgccct tcgggaccag atccttgtcc
     4741 tattggcgtc ctcagtgggc gcagtccaag tcacagggct ccagcgatgg acagaggcga
     4801 agaatctcgc gcactttcga aataccctca ggggtctgca caaggcccgg ctgatcgaac
     4861 tgtcgcaggc caaaatggag gttcaactct tgccgcctgg ttctaaggaa gcttcgttac
     4921 ccgtcgcaca ccacattgcc tgagctatcg cgccggtcac gtttggcgcc ctgaatgtac
     4981 aaaatcgtct tagacgccaa cgtcatcgtg tctgccctcc agtcccggcg cggagcgtct
     5041 tttgccatcc tccagcggat cggcgaagcc tggcaacctt tggtctccgt gccgctcatc
     5101 tttgaatacg aggggtgggc aagagtgaag ctgagaggct ggtgacttgg atatgctgga
     5161 gtccgtgcgc agcatcggaa cggagcaaaa tcaacagaaa agagaacttt atgccgcgtt
     5221 tcattggaat ttttgccgga cttcggaaaa acggtcttcc ggcgctcgcc acaatctcga
     5281 gcgaatgcag gcagctggca tagtggaacc ggaggtcgaa gcccgtaaaa tgcaggctgc
     5341 gccttgggcg ccagtttccg cggtccccaa cgaaatacac cagattattg cgtgttcacg
     5401 cggcgctgct cgcggtattc gcgaggcgtc atgtgcacga gcttgcggaa tatatagccg
     5461 aaatagctct ggtcgcagaa gccgacttcc tggctgactt gtgcgatcgt cttatttgtc
     5521 gaggcaagca gcgcttccgc cttcgcgatg cggaaacggt tcagatacga catcagcggt
     5581 tcgcccgtaa cccggcggaa caaacgcatg aagtgcgaag gactcaaatg cagaatcgcg
     5641 gccgcctgct ccagcgtcag gcgttcggaa taatgacgct cgataaagtc cagaaggggt
     5701 ttgaggcgtt caaaatccct ctcccggcga tgcaaaaacg tcgtggagct gagcgtggcg
     5761 gtgtagtaat tcatcaacag gaccaggatc atcttgagat atgtcttggc caccaagcga
     5821 ttccggtcgg atcgtgccgg aagctccgag cggatccttt ccatcaaatc gcgaacctga
     5881 gccggaacgc cggtggctgc cggcacgacg tgagaaaatt cctcgttctg cacgagaaac
     5941 ggcgtcagat attcgatatc ctcgtgcgtc atatctccgg cccggatcag ctcgggaaga
     6001 aaatgcagcg ctattcctct gccgtcttcg caaacatagg tatgcacgcg gtgtagcagc
     6061 gcgctcccca taacaaagag atcgcccgcc cgcatcgcga ccagccggcc ctcaacttcg
     6121 aaaacaattt ccccgacctc gagaaatacc agctcaaaat acgcatgccg gcacagttcg
     6181 gcatgaaaat cgcgagccaa tgccggctgc acttccaggg gaaagctctg gtcgaacggc
     6241 caggataaaa tttcggctgc cgccattccg gccggcgcga cggcggccgg aagttccgaa
     6301 gcactcttct tgctgtatag ggcccccacg aatcaccccc gattcgtatt tatgcccctt
     6361 tcaccccggg gagacaacgc tggtatttta tagcattatc ccccggcgga tacaataggc
     6421 atttttaatt tggcgcggct cgtggtagtc tttacggcgg cgcccgcccg ccggagtccg
     6481 ccattgcccg gccctcaggg agcctcctgg tcccgtggtt gccggggggg cgtcggccgc
     6541 acagtctggc aggccaattg gacgggccgg caaggtggct caggaagccg gccagtgcgg
     6601 ggttacgccg ccgcgtcagc ggcgccgggg gcaggactct gcggctattc tcttgctgta
     6661 attgttcgcc atctgatcga ttttgaccat gatactggac tcggtttgaa ggagggaaag
     6721 tggataacgt agatcgacga gacttcttgc ggggcgccgc cggcgcattc gcagcggctt
     6781 cggcccttgc ggctccggcc ggaaatgaaa aggttcgggt gggcttgtac ggcacccggt
     6841 attcgcattt gatcggaaaa atcggaatta tgaaacgagc ctcggatacg ttcgaaatcg
     6901 ccggggctta tgaaccggaa gggacgccgc cggataaccc cgcttttaac ggaatcaaaa
     6961 tagttagcga ggatcaactg cttggcgatc cgagcatcca tctgatcatc gtggaagggc
     7021 aggtcgacaa aactgtcccc tgggcccgca aagcgattgc cgctaacaag cacgtccacc
     7081 tcgagcgccc cccgggcgtc gagatggagc cgttccgcca actcgtcgaa gaggcccgcc
     7141 gccggaaatt gctgattcag gtcggctacc cgtggaagtt ttcgccgggc gttgtggcgg
     7201 cattcgaggc cgtccagaag ggttacctgg gcgacgttta catggttcgc gccacggtga
     7261 acaccgatat tgacgcccgc gcccgcgccg gcatgcagcg tttcaaaaca ggcatttttt
     7321 acgatctcgg gccctacatg gtggacctcg tggtggcgct gctcggcaag ccaaaagagg
     7381 tgaaaggctg gttccatcac gacgactcgg gctttccaga caacatcatt gacaacagcc
     7441 tcgccgtttt ggaatatccg aaggcttttg ccgtcgtgag ctgcaacgca aggcaagcgg
     7501 gatcccggga acagcggcag tttgaaatca tcgggactga tggctgggca aacttgcagc
     7561 cggtcgagcc cggcagactg cacctgcatc tgcgcgaagc ccgcggcccg tataaagccg
     7621 gacctcaaga ggtggcgctg ccggttccgc cggtcccccg ctggaccggc gatcttctcg
     7681 aaatggctcg ggccatacgc acgggcacgc cgctcaagta ttcctacgat cacgaattgc
     7741 tcttgcagga gaccgtgatg cggattaccg gcgggcgcgg gtagctcctc cgcaacccga
     7801 ttcggaagcg cggcgaagcc gaaaggaatt gcggctttgg ctgtggtttt tcgcttcgga
     7861 tgtggttttc gaagcctcag cccacgtgcg ggcgaagatc ccccgcgaaa aatcctgttt
     7921 ccgcgggctc agccaggcct ggatcccaaa tcgccttcat ttcgcggcgc cctggcccgg
     7981 agctcgaaaa taaactcgct gactggtgat ggcttcactc ccgccgtgtg ctatagtaaa
     8041 ggtgcgtttg aagctcttgg ctttcgtttt gatccgatct tgacaaaacc cgcggcgcaa
     8101 ctgccgcgcg gtttcttttt tgatcctcag ccgaccagca ggagttcgaa tagctcaaca
     8161 aaggattttg tgtttcatga aagaaaaagg taccgtcaag tggttcaatg cggccaaagg
     8221 ttttggcttc attcagcgag acaacggaga cgatgttttc gtgcatttct ccgcgattca
     8281 ggcgaacggc tatcgcagcc tggacgaggg tgcgcaagtg gaattttcgg tcaagaaggg
     8341 tcccaagggc cttcaggccg aagatgtagc tgtcgtctga tttgtttcga gcgctttgct
     8401 tccggggcgc acctcatgcg cctcggatct cgagctacgt cctacagttc ccgcctcccg
     8461 agacgcattg ccttttgcgc cggagtgtcc taatctaaag gctgctgatc cctccatcgc
     8521 cattacccgg gcggctttgc tgagatcaac gaatccatca aaggaacggg agtgtcccta
     8581 tgagtggaac gataaaaaaa ttgatcgccg agagaaactt cggctttatt cgcgccgaga
     8641 atggccagga cgtttttttt cacgcctcgg cggtacagca aaacggcttc gcctcattgg
     8701 ccgttgggca ggcggtgaac ttcgatctgg aacgcggcga aaaagggccg aaagcctcga
     8761 atgtgcgggc gcggcgggaa cagcctttgt catgaccgcg gcgaaacggg agacgctatg
     8821 caactgcaat acataggatt cacccagacc gagaataccc gagaatacat tttccacggg
     8881 ctggtgcacg gcgagacaac ccgggttttt gtcatgagcg cggatatggc attgttcctc
     8941 aaacatcgtg tcggcgtgca ggaaggcccc gtactgtgct tgcgcacgct gaccgccgag
     9001 ttgggggcgc aggaccag
//
LOCUS       DASREK010000030         6149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1335947, whole genome shotgun sequence.
ACCESSION   DASREK010000030 DASREK010000000
VERSION     DASREK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335947"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFA54_01920"
     CDS             <1..628
                     /locus_tag="VFA54_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49588.1"
                     /translation="VGKTIRQAKRGNTLGPLTLSLFYATDFADRLENEIIPALRAGFV
                     VLTDRYIYSSIARASARGSDPAWIRQVFGFALRPHAIYYLRASVQDLLPRVVYAHGGF
                     DYWESGMDVHMGKDLFESFVRYQSRVIRALDSMAQRYNFEIVDASRTPDKIFQDLKKR
                     ITRLLDGAKRRPRGPAAPSRARKKPVRAPEPVSAGAPPSVDLAAPVPA"
     gene            759..1574
                     /locus_tag="VFA54_01925"
     CDS             759..1574
                     /locus_tag="VFA54_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS49589.1"
                     /translation="MALLDSKVALITGAARGIGRAAAELFAREGARLVINDLDKEPLR
                     EAAEALRRAGAETAVLPGSVTDRDFPEKWIQCALDRFGRIDIIVNNAGLTWDAVIHKM
                     TDEQWDRIIDLHLKAPFRIIRAAAPYLRETAKSEALRGPIPCRKIINVSSTSGVAGNA
                     GQANYSAAKMGIVGLTKTVAKEWGPFNITCNAVAYGFIDTRLTQEKEKGVTAGEGIVL
                     GIPRQAREIVLRQIPLGRAGTPQEAAGPVLFLASPLSDYVTGAVLLVTGGLYM"
     gene            complement(1631..2560)
                     /locus_tag="VFA54_01930"
     CDS             complement(1631..2560)
                     /locus_tag="VFA54_01930"
                     /inference="COORDINATES: protein motif:HMM:NF044297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZS49590.1"
                     /translation="MIDQNRQLRAGESLCGSWAAFKLCSYLIQFTGEARYRDWIEKLF
                     YNGAGAALPLQPGGRNFYYSDYRVGGGMKVYRWDNFTCCSGAYFQDMADYHNLIYYKD
                     GSSLYVNLYVPSQVAWNHRGTDIKVTQQTDYPVSETTTLSLEMPQSVEFALNLRVPEW
                     CRGMSVNVNGSAVNAACQPGTWATVVRRWNSGDRVEARIPVRLRMQAVDRQHPDRVAV
                     VRGPVVLALDFDYHDPAFELPGTGDELNKWLVRDDSPLPSHFSAPAVPGMFRVERPDG
                     RNVRLRFRPFYAYEEGFPYLMYIDRKAWPYRLW"
     gene            2607..3335
                     /locus_tag="VFA54_01935"
     CDS             2607..3335
                     /locus_tag="VFA54_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZS49591.1"
                     /translation="MPLPTSPVSFSDRRLSDRLEQLSNSIGKKVPISHQIAHTIRDLI
                     ISGTLNPGDRIIESKIAKQLSVGQPTVREALVALEHQGLVVRKSNQGCVVTTLTRNEV
                     CQILEVRPELEGLAVELGAKKGRQQDLEHLASAARRMKAAAQAQNIEEFYSHDLAFHN
                     LLWKLPGNTVLTKLLEELMLPLLALCYLRNLRNRAYVDMTASAEAHNRIAAGLASRKA
                     SKARQIALQSFEIFAEQHLRYFEG"
     gene            complement(3727..3803)
                     /locus_tag="VFA54_01940"
     tRNA            complement(3727..3803)
                     /locus_tag="VFA54_01940"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3767..3769),aa:Arg,seq:tct)
     gene            complement(4002..>6149)
                     /locus_tag="VFA54_01945"
     CDS             complement(4002..>6149)
                     /locus_tag="VFA54_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49592.1"
                     /translation="MTYVISPTMTNEFNWGFTKNKIDIFEVGNKLRRKTSGVNLPLLY
                     PNAVQDDYIPEPRFDGTHLANSPQFVANDAPFHNYNTSIDIVDNLTKIWGAHAIKTGI
                     FMQRSRKDQTSFGDNNGYYRFGDNPSNPYDTGYGYSNALLGVYQSMDQASAYINGMYR
                     YWNIEGFVQDTWKITPRVTLDYGLRISWYQPQYDSSLQASTFVLSAWDPAKAPRLYGP
                     TTINGQRMAIDPVTGQVLPAYAVGLEVPGSGDPFNGILQAGKGINKYLQQDRGPQWGP
                     RFGIAWDVTGKQNFVIRTGGGVYYDRFQGNRVFDFVRNPPESVQPTLMYGFAQDISPS
                     NILLGPPTLYAADPIAKIPTTYNYQFGIQTRLPWSMMLDTAYVGSLARHLQDNRNLNP
                     VPYGATFLPQNQDPNKVAANPNALPGSNALPDVFLRPLRGYGQINLYESNSTSNYNAL
                     QINLTRRLGRGVTFGIAYTWSKALTTASSDTTWVRADNFTRAADYGPASFDRRQVFAA
                     NYIYNLPGTHVGNAFARLLTSGWQLSGVVLASTGSPFTPGFSISGAGSQNITGNFVAN
                     STSEGARIGVRPGCDPYTHSSDPFNRLNAACFFAPQPGSLGLESGVNWLYNPGLIDFD
                     MSLQKEFAVRERVRLQFRVDAFNVFNHPNFTGLNTTLNFTSYPNPVIANNATPYNAAG
                     QLVNVTGFGAVTVPAPGAAGSSRILQTLIRIQF"
BASE COUNT         1258 a         1851 c         1821 g         1219 t
ORIGIN      
        1 ggtgggaaaa acgatccggc aggcaaagcg cggcaacact ctgggcccgc ttaccctaag
       61 ccttttttac gccacggatt tcgccgaccg gctggagaac gagatcattc ccgccctccg
      121 cgcgggtttt gtggttctga cggatcggta catttactcc tcgatcgcgc gggcgagcgc
      181 caggggcagc gatccggcat ggatccgcca ggtgttcggt tttgccttgc gccctcacgc
      241 catttactac ctgcgggcca gcgtgcagga cctgctgccg cgcgtggtct acgcgcacgg
      301 cggattcgac tactgggaat cgggcatgga cgtgcatatg ggcaaggatc tgtttgaaag
      361 ctttgtccgt taccaaagcc gcgtaatccg ggctctcgat tcgatggcgc agcgttacaa
      421 cttcgagatc gtggacgcat cccgaacgcc cgacaagatc tttcaggatt taaaaaagcg
      481 gatcacccgg ctgctagatg gcgccaagag gcgtccgcgc gggccggccg cgccctcccg
      541 ggcccgtaag aagccggtgc gcgctcccga gcccgtttcc gccggcgccc cgccctccgt
      601 cgatttggcg gcgcccgtcc cggcctgagc ctgccggtct cgggcaagcc tttgccgatc
      661 gcaaaccgag ccattcccgg gcatcagttt gctgcccggc cacagtctgg gcaatgcaga
      721 tgttatcttc aaatttctta tccggaacgg gagacgcaat ggcgctgctg gatagcaaag
      781 tcgctcttat caccggagca gcgcgcggca tcgggcgcgc cgcggcggaa ctgttcgcgc
      841 gggaaggggc gcggctggtg atcaacgatc tggataagga gccgctgcgc gaagccgccg
      901 aagcattgcg ccgcgccggc gctgaaaccg ccgtcctgcc gggcagcgtg acagaccgcg
      961 attttccgga aaagtggatc caatgcgccc tcgaccgttt cggccgtatt gatatcatcg
     1021 tcaacaacgc gggactgacc tgggacgcgg tcattcacaa gatgaccgac gagcaatggg
     1081 accgcatcat cgacctgcac ctgaaagcgc cgtttcgaat catccgcgct gcagccccct
     1141 atctgcgcga gacagcgaaa tccgaagcct tgagaggccc gatcccctgc cgcaagatca
     1201 tcaacgtcag ctctacatca ggcgtcgccg gcaatgccgg ccaggcgaat tactctgccg
     1261 cgaagatggg catcgtcgga ctgaccaaaa ccgtcgccaa ggaatggggt ccgttcaata
     1321 tcacttgcaa cgccgtcgcc tacggcttca tcgacacgcg gctgacgcag gaaaaagaaa
     1381 aaggcgtcac tgcgggcgaa ggcattgtgc tgggaattcc gaggcaagcg cgcgagattg
     1441 tcctgcgcca gattccgtta ggccgcgctg gcacgccgca ggaagcggcg ggaccggtgc
     1501 tgtttctggc ctctccgtta tccgattacg tgacaggagc cgtcctactg gtcaccggcg
     1561 gcctttatat gtagcgccag agcggccgcc tgccgccgag ccttgcttcg agcgaaacaa
     1621 gaggaagggc ttaccagagc cggtagggcc aagcttttcg gtcgatgtac atcaggtacg
     1681 gaaatccttc ctcatacgca taaaacggac gaaatctcag ccgcacattg cggccgtccg
     1741 ggcgttccac ccgaaacatg ccgggaacgg cgggagccga aaaatgcgac ggcaggggac
     1801 tatcatcgcg caccagccat ttgttcagtt catcgcccgt gccgggaagc tcgaatgccg
     1861 gatcatggta gtcgaaatcg agggccagca ccaccggccc ccggaccaca gcgacgcggt
     1921 cggggtgttg ccggtccacc gcctgcatgc gcaggcgcac ggggatgcgc gcttccacgc
     1981 gatcgcccga attccagcgg cgcacgaccg tggcccaggt tcccggctgg catgctgcgt
     2041 ttactgcact tccgttcacg ttcaccgaca tcccgcgaca ccactccggg acgcgcaagt
     2101 tcagcgcgaa ttctacgctt tgcggcattt ccaggctgag cgtcgtggtc tccgaaaccg
     2161 gataatcggt ctgctgggtc actttgatgt cggttcccct gtggttccag gcaacctgag
     2221 agggcacgta gagattgaca tacaggcttg atccgtcctt gtagtagatc aagttatgat
     2281 aatcagccat gtcttgaaaa taggcgccgg agcagcaggt gaagttatcc cagcggtaga
     2341 ctttcatgcc tccgccgacc cgatagtcgg aatagtagaa gttccgccct ccgggctgga
     2401 gcggaagcgc cgcgccggcg ccgttgtaaa acagtttctc gatccagtcc ctgtatcgcg
     2461 cctctccagt gaactgtatt agatagctgc acagtttaaa agccgcccaa gagccgcaca
     2521 gggactcgcc agctcgcagt tgccgattct gatctattat cttttatctt tacgggaacc
     2581 gccgatcaat tgtaaactcg aggtttatgc ctctcccaac atcgccggtc agcttctctg
     2641 acaggcgcct ttccgacaga ctggaacaac tttcgaattc catcgggaaa aaagtcccca
     2701 tttcccatca aatcgcccac accattcgcg acctgatcat ttcggggacg ttgaatccgg
     2761 gagacaggat cattgaatcg aaaatcgcca agcagcttag cgtcgggcag cccacagtgc
     2821 gggaagccct agtcgcgctc gagcatcagg ggctcgtggt acgcaaatcc aaccagggct
     2881 gcgtggtgac cactcttacc cgcaatgaag tttgccaaat cctggaggtg cggccggaac
     2941 tggaaggtct cgcggtggaa ctgggggcca agaaggggcg gcagcaggac ctcgagcacc
     3001 tcgcctccgc cgcccgccgc atgaaagccg ccgcgcaggc ccagaacatc gaagaattct
     3061 acagtcacga cctggcgttt cacaacctcc tttggaagct tcccggcaac acggtcttga
     3121 ccaaacttct ggaagagctt atgctgccgc tgctggcgct ttgctatctg cgcaatcttc
     3181 gcaatcgagc ctatgtcgat atgacagcct cggcggaagc tcataaccgg atcgccgccg
     3241 gactggcctc ccgtaaagcc tccaaggcca ggcaaatcgc gctccagagc ttcgagatat
     3301 ttgccgagca acacctgcgc tacttcgagg gatagttccc gagaagatcg ccccgccccg
     3361 ggggcatacc ggtctcggcg gcgcggaatc aatagcggcc tccaccagag tttcaaccac
     3421 gcggctgacc ctgctcgacg caactatgat catcgtgggg tcgatgatcg gcggcggcat
     3481 ataccaaggg cagagtggcg ctcgggctgg ccgctcggcg ggcctgcaat cgccgaaggc
     3541 gggccactcc tggggaagct ccccacgttg gtcgaaagca ccattgtctg cggcctcccg
     3601 cgccattccg cgcacaagcg aactccgccc cgcaaaaacg cccccaaaac gccgctccct
     3661 gcaaacatct gtgtggacat ggcgctcttg acgttcaaat cagccgcgct aagcgactga
     3721 aagggatggt aggcgcggca ggactcgaac ctgcgaccct ctgcttagaa ggcagatgct
     3781 ctatccacct gagctacgcg cccctgtcgt catcctacca gctaccctcc ttcggctcaa
     3841 tccacggctt cgccggagcc gttaacacat gatctgtccg ctgccggcca gcagccctcc
     3901 tcgttaagac tccccattaa tacaacgagg ccctcgctgt tttatccagt taggcggccg
     3961 aggggaacta aggggcggcg cgcttccgcc cgcgccccgt gttagaactg gattcggatc
     4021 agcgtctgca aaatccggga ggatcccgcc gctccgggag cgggaaccgt aacggcgccg
     4081 aacccggtca cgttgaccaa ctgtccggcg gcattataag gggttgcatt gttcgcaatc
     4141 accggatttg gatagctggt gaagttcagc gtcgtgttca aacctgtgaa attcgggtga
     4201 ttgaagacgt tgaacgcgtc cacgcggaac tgcaagcgga cccgctccct aacggcaaac
     4261 tctttctgga gcgacatatc gaaatcgatc agcccggggt tgtagagcca gttcactccg
     4321 gattccaatc ccaggctgcc gggctgcgga gcgaagaaac aggcggcatt gaggcgattg
     4381 aacggatcac ttgagtgggt gtagggatcg cagcccggcc gcaccccgat gcgggcgcct
     4441 tccgaggtgc tattggcgac aaagttgccg gttatgttct ggctgcctgc ccccgaaatg
     4501 ctgaatcccg gcgtgaatgg ggaacccgtg gaggccaaaa cgacgccgga gagctgccag
     4561 ccgctggtga gcagccgggc aaaggcgttc ccaacatgcg ttccgggaag gttgtaaata
     4621 taattcgctg caaatacctg ccgccggtcg aagcttgccg gaccgtaatc ggcggcgcga
     4681 gtgaagttat ccgcccgcac ccaggtggtg tcggaggagg cagtggtgag ggccttgctc
     4741 caggtatagg cgatgccgaa ggtaaccccg cgccctaagc gccgggtcag attaatctgt
     4801 agagcattat aattcgacgt cgaattgctc tcatacaaat tgatctggcc ataaccgcgc
     4861 aatggacgca ggaaaacgtc cggcagagca ttgcttcccg gcagggcatt cggattcgcg
     4921 gctactttgt tcggatcctg attctgaggc aggaatgtgg cgccgtacgg gacggggttt
     4981 aggttgcggt tatcctgaag gtggcgggcg agcgacccca cgtaggctgt gtccagcatc
     5041 atactccacg gcagccgggt ttgtatcccg aactgatagt tgtaggtggt tggaattttg
     5101 gctatcggat cggcggcata gagcgtggga ggcccgagca ggatgttgct ggggctgata
     5161 tcctgcgcga acccgtacat caacgtcggc tgcaccgatt cgggcggatt tcgcacgaag
     5221 tcgaatacgc gattcccctg aaaccggtcg taatagacgc ccccgccggt tcggatcacg
     5281 aagttctgct tgccggtgac gtcccaggcg atgccgaagc gaggccccca ctgcggaccg
     5341 cggtcctgtt gtagatattt gttgatgccc ttgccggcct gcaatatgcc attgaacgga
     5401 tcgcccgaac ctggcacttc aaggcctacg gcatatgcgg ggagaacctg tccagtcacc
     5461 ggatcgatgg ccattcgctg tccgttgatt gtggtcggcc catacaagcg cggcgccttg
     5521 gccggatccc acgcgctcaa cacgaacgtc gaagcctgca gcgacgaatc gtattgcggc
     5581 tgataccaag agatgcgcag gccgtagtca agagtgaccc gcggcgtgat cttccacgtg
     5641 tcctgcacga acccttcaat gttccagtat cggtacatgc cgttaatata ggcgctggcc
     5701 tggtccatgc tctggtacac gccgagcagc gcattcgaat agccgtaacc ggtgtcgtac
     5761 ggattgctcg ggttgtcgcc gaaccggtaa tacccgttgt tatccccaaa cgaggtctgg
     5821 tctttgcggc tgcgctgcat gaaaatgcct gtcttgatgg cgtgagcgcc ccaaattttg
     5881 gtcagattat cgacgatatc gatgctcgta ttgtagttgt gaaacggcgc gtcgttggcg
     5941 acgaattggg gcgagttcgc caggtgcgta ccatcgaaac gtggttcggg gatatagtca
     6001 tcctgcacgg cgttgggata cagcagcggc aggttgactc ccgacgtctt gcgtctcagc
     6061 ttattgccga cctcgaagat atcgatctta tttttggtga agccccagtt gaattcattg
     6121 gtcatcgtgg ggctgataac ataggtcat
//
LOCUS       DASREK010000031         2856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2161170, whole genome shotgun sequence.
ACCESSION   DASREK010000031 DASREK010000000
VERSION     DASREK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161170"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFA54_01950"
     CDS             complement(<1..312)
                     /locus_tag="VFA54_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZS49593.1"
                     /translation="MPPETGEAAGEAPGAGLMRAAVYRGAGRVCIETVPIPKIGPGEL
                     LVRVEACGICHTDLKKIAHDLLPPPRIYGHETAGVVVEAGAGVQEFRPGDRVALFHHI
                     PC"
     gene            complement(317..994)
                     /locus_tag="VFA54_01955"
     CDS             complement(317..994)
                     /locus_tag="VFA54_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49594.1"
                     /translation="MSGVHAVVKTGASEGTQKGLAWCRRGSLRRRSIVLAANGAGPSN
                     AARAVDRISDLMRVAAVVSTGLCGALDPALLPGDLVAAERVLSLDPPLEFAARPPGGC
                     SAPPARKGTVLTVDHVVQSAGEKRRLRATGASVVEMEAAGVAAEACKRGLPFFCVRAV
                     SDGAEESFGIDYNRARRPDGSFSPADIVAQAGLRPARWAELIQWRRRRRLAAERLGDF
                     FASSEFD"
     gene            complement(1039..2034)
                     /gene="hpnA"
                     /locus_tag="VFA54_01960"
     CDS             complement(1039..2034)
                     /gene="hpnA"
                     /locus_tag="VFA54_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HZS49595.1"
                     /translation="MILVTGASGFIGGHVARLLVERGERVRILVRPGSDLRAICDLLD
                     GKHAVERVYGDLREPESLRRAAAGCEVAYHIAADYRLWARDPEEIYRSNLEGTRNFLE
                     AARSEGVRKVVYTSTVGCIGVRGDGTPGDEESPVGLEDMAGAYKRSKFLAEQVALEFA
                     REGLPVVVVNPTAPVGERDVKPTPTGRIIVDFMLGRMPAYVDTGLNLVDVRDVARGHL
                     AAAERGRSGQRYILGGRNMTLREIVEELARLGGRAAPKVRIPYALAWMYAAAGTAAAR
                     VTGREPRAPLDAVRMSRKKMFVRTEKAERELGFAPGPVEEALGRAIEWFRKNQYC"
     gene            complement(2110..>2856)
                     /gene="hpnH"
                     /locus_tag="VFA54_01965"
     CDS             complement(2110..>2856)
                     /gene="hpnH"
                     /locus_tag="VFA54_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HZS49596.1"
                     /translation="PQIGQLVEGILQRRKHIYLCTNGMFIVKRLHEFRPHRSFFFNVH
                     LDGLEKTHDMCVEREGVFREAIEGIKAAKKAGFLVCTNTTVYRETDMKEIEALFEYLQ
                     QFGVDGHMLSPGYGYSAVDDREIFLTRAEIREKFKDVDKLFRKFKLNNTPAYLDFLKG
                     ERELPCTAWGNPTYNIRGWKGPCYLITDAHYKTFEELMSQTPWERYGHGGPDPRCQDC
                     MVHCGYEPSAALGINARLGDSFRMLSWALQ"
BASE COUNT          527 a          996 c          806 g          527 t
ORIGIN      
        1 gcacggaatg tgatggaaca gcgctacgcg gtctcccggg cggaactcct gcacccccgc
       61 gcctgcctcc acgaccacac cagcggtttc atggccgtag atgcgaggcg gcgggagcag
      121 atcgtgcgcg attttcttca aatcggtatg acagatgccg caggcttcca ctcgcaccag
      181 taactcgccg ggcccgattt tcggaattgg aaccgtctca atgcacaccc gacccgcgcc
      241 ccggtacaca gccgcgcgca tcaagcctgc gccaggcgcc tcgccggccg cttcaccggt
      301 ttcgggcggc atgggctcag tcaaattcgc tgctcgcaaa aaaatctccc aaacgttcgg
      361 cggccagccg cctgcggcgc cgccattgga ttagttcggc ccagcgcgcg ggccgcagac
      421 cggcctgcgc gacgatgtcc gcgggcgaga aactgccgtc aggtcgccgc gcccggttgt
      481 aatcgatccc gaaagactcc tccgcgccgt cgctcaccgc ccggacgcaa aaaaacggca
      541 ggcccctctt gcaggcttct gcggcgacgc cggcggcttc catttccacg actgacgccc
      601 cggttgcgcg caaacgccgc ttttcaccgg ctgactgcac tacatgatcg actgtcaaca
      661 ccgtgccctt gcgggccggc ggcgcagaac agccgccggg cgggcgagcc gcgaactcga
      721 gcggcgggtc gagcgagagc acgcgctccg cggccaccag atctcccggc agcaacgctg
      781 gatccagcgc gccgcacaag ccggttgaaa ccaccgcagc cactcgcatg agatcgctaa
      841 tccgatccac cgcgcgtgcg gcgttgctag ggcccgcgcc gttcgcggct aaaacaatgc
      901 tcctgcgccg caggctgccc cgccggcacc atgcgaggcc cttttgtgtg ccttcagaag
      961 cgcccgtttt tacaacagcg tgaacgccgc tcattttatg agcgacgccg gccagttccc
     1021 gccaatcagc ggcgacgatc agcagtactg gttcttgcga aaccactcaa tggcgcggcc
     1081 tagcgcctct tcaacaggcc cgggagcgaa acccaactcc cgctcggcct tttctgtgcg
     1141 gacaaacatc tttttccgcg acatccgcac cgcatccagc ggcgcccggg gttcgcgccc
     1201 ggtcactcgg gcggcggcgg ttcctgccgc cgcatacatc cacgccagcg cgtacggaat
     1261 ccggactttc ggcgccgctc ttccgcccag ccgggctaac tcttccacga tttctcgcag
     1321 cgtcatgttg cgcccgccga gaatataacg ctggccgctg cggccccgct cggccgccgc
     1381 cagatgtccc cgcgccacgt cgcggacatc cacgagattc aacccagtgt ccacgtaagc
     1441 cggcatacgg ccgagcataa aatctactat gatgcggcca gtgggcgtcg gtttcacatc
     1501 tcgttctccg accggcgccg tcgggttgac gaccacgacc ggcaagccct cgcgcgcgaa
     1561 ttccaaggcg acctgctcgg cgaggaactt ggagcgctta taagcgcccg ccatgtcttc
     1621 caaccccacg ggggattctt catcaccggg ggttccatcg ccgcgcacgc cgatgcagcc
     1681 caccgtgctc gtgtatacaa ccttgcggac tccctcgctg cgggccgctt ccaggaagtt
     1741 ccgcgtgccc tcgagattag agcggtaaat ttcttccgga tcccgggccc acagccggta
     1801 atcggccgct atgtggtacg cgacttcgca cccagcggcc gcgcgccgga gggattcggg
     1861 ctcgcggaga tcgccgtata ctcgctctac ggcatgcttg ccatccagca gatcgcaaat
     1921 ggcgcgcagg tcgctgccgg gtcgcaccag aatccggact cgctcgccgc gctcaaccag
     1981 cagccgcgcg acgtggccgc caataaatcc cgaagcgccg gttacgagaa tcatacggta
     2041 gagccggctt ggctgccatt gaggcgggac aagccgcgcg tatctgtctt cgccccgcct
     2101 ttcgaatcat cattgcagcg cccaactcag catccggaag ctgtcgccga gccgggcgtt
     2161 gatgcccagg gccgcggacg gctcataccc gcagtgcacc atgcaatcct ggcagcgcgg
     2221 atcgggcccg ccatgaccgt aacgctccca aggcgtctgg ctcatcaact cctcgaaggt
     2281 cttgtaatgg gcgtcggtga tcaagtagca cggtcccttc cagcccctga tgttgtacgt
     2341 cggattcccc cacgcggtgc agggcaattc ccgttctccc ttgagaaaat caagataggc
     2401 cggcgtattg ttgagcttaa atttccggaa aagcttgtcc acgtccttga acttctcccg
     2461 gatttccgcc cttgtgagaa aaatctcccg gtcatcgacg gcagaatacc catagcccgg
     2521 agaaagcatg tgcccgtcca ctccgaattg ctgcaagtat tcaaacagcg cttcgatctc
     2581 tttcatgtcg gtttcccggt agacggttgt gttggtgcaa accaggaaac cagccttttt
     2641 tgccgctttg atgccctcaa tcgcttctcg aaacacccct tcccgctcga cgcacatgtc
     2701 gtgggtcttt tccaaaccat ccaagtggac gttgaagaag aaactgcggt gcgggcggaa
     2761 ttcgtgcagc cgcttcacga tgaacatccc gttcgtgcaa aggtaaatgt gcttcctgcg
     2821 ctgcaaaatt ccttccacga gctggccgat ctgcgg
//
LOCUS       DASREK010000032         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2161986, whole genome shotgun sequence.
ACCESSION   DASREK010000032 DASREK010000000
VERSION     DASREK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161986"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFA54_01970"
     CDS             <1..219
                     /locus_tag="VFA54_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49597.1"
                     /translation="ARFDGTISFAVLVLSAGSSGVPEDSVPLVNRTVPSGKNVGALPA
                     ALGDRQANGIIPSFHKLDAWFRPDHASK"
     gene            223..1170
                     /locus_tag="VFA54_01975"
     CDS             223..1170
                     /locus_tag="VFA54_01975"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HZS49598.1"
                     /translation="MPALPLFRKFSQAARRNELAWRYTFNLGPALKFRMARTGLPAEA
                     ARVVRDLDRQGIAMIPADKLLGESSLFAELQSAVASLEQSLSRQLQEARQAADDPLAG
                     REKAFLYELLGPRPALDPSSIFARFALSRPILQIANAYFGMYTRLRYYNVWHTFVTSG
                     QPRQSQLWHRDREDRLILKLFVYLDDVDLEAGPLTYAPGTHLKGAIRQTPECFQESGV
                     WRSTDEQMAAVVPRERWISAVGGKGAIVFADTHGYHKGGLARGRDRILYTCMFTSPAS
                     ESAELLSRPKILPLPAEKELAFALSAPKRGPLAAWRPRG"
     gene            1175..>2527
                     /locus_tag="VFA54_01980"
     CDS             1175..>2527
                     /locus_tag="VFA54_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49599.1"
                     /translation="MPAIHSAPNSTPCVEALRSASEETGHCRPEVLLLSTSLLTDRML
                     FQTRFLDVLAAEAGVRVWTSSVRRPRPSREASAAGPIAVEEFPEVRPFREFPYNYLRR
                     LNEFAWDFRQRTPTRLSLMRRIRDKMQPPSVRAMKLPARAVALLGAEQWLEDRLEGLL
                     LSYPRSPEAVQRLKQLRPALLVATGPFWFLEPGVVAAAKTLGIPCLALIPSWDNLSTK
                     NRMVFQYDGYLVWSEQAREELRAFYPSSRPVPAYVVGAPQFDVFFQRRFHQSREDFCR
                     KHGLRPGCPIILYAVGSPNLIREQHGAVYMAERVARGELGEVQLLIRPHPIHDNGEMN
                     QLFGRFGPRVVLQQTGEAGLAVAARRQDEDQIAAWVNTFRHADVVVNLSSTVAIDAAI
                     MDRPVVNLDYDPEPGQPNQGLVKDINHTWTHFKPVAESGGVWLVNNPEEMVHAVKTYL
                     R"
BASE COUNT          447 a          798 c          778 g          504 t
ORIGIN      
        1 gcgaggtttg acgggacgat cagctttgcg gttctggtcc tctccgcagg ctcatccggt
       61 gtaccagagg actccgtccc cctggtgaat cgaaccgtgc cctctgggaa aaatgtcggt
      121 gcgctgccag cggcgctcgg cgaccgtcag gcgaatggca tcattccgtc gtttcataag
      181 ctagacgcgt ggttccgtcc ggaccacgct tccaagtaag ccatgcccgc attgccctta
      241 tttcgcaaat tttcccaagc cgcgcgccgg aatgaactgg cctggcgcta cacattcaat
      301 cttgggcctg ctctgaaatt ccggatggcc cgcaccggat tgcccgccga agccgcgcgc
      361 gtcgttcggg atctcgaccg gcagggcatc gcaatgatcc cggcggacaa actgctcggg
      421 gaaagcagct tatttgccga actccagagc gcggtggcga gtctggagca aagcttgagc
      481 cggcaactgc aagaagcccg ccaggccgcc gacgatccgc tcgccggacg cgagaaggcc
      541 ttcctctacg aactgctcgg gccgcggccc gctctcgatc ccagcagcat atttgcccgg
      601 ttcgccctga gccggccaat cctacagatc gccaacgcat actttggcat gtacacgcgg
      661 ctgcgctatt acaatgtatg gcacactttc gtcacctcgg ggcagccgcg gcagagccag
      721 ctttggcatc gcgaccgcga ggaccggctt atcctgaagc ttttcgttta tctcgacgac
      781 gtggatttgg aagccggacc cctcacctac gcgccgggca cgcatctgaa aggcgcgatc
      841 cgccagaccc ccgagtgctt ccaagaaagc ggcgtgtggc gcagcacgga cgagcagatg
      901 gcggcggtgg ttccccgcga gcgctggatc tcggcggttg gagggaaagg cgccatcgtt
      961 ttcgccgata ctcacggcta tcacaaaggc ggcttggcgc gcgggcgcga ccgcattctg
     1021 tatacatgca tgttcacctc gcccgcatcg gaatcggccg aactgctatc tcgtccgaag
     1081 atattgccgc tgcccgcgga aaaagaactg gcgtttgctt tgtcggcgcc gaaacggggg
     1141 cctttggccg cctggcgtcc tcggggctaa atttatgccg gcgatccact cggcgccaaa
     1201 ctcgaccccg tgcgtggaag cgcttcgttc tgcgagtgag gagaccgggc attgccggcc
     1261 ggaagtcctc ctgctttcga cgtcgcttct gaccgatcgg atgctgttcc agacgcgctt
     1321 tctggacgtg ctcgcggccg aggcaggggt gcgcgtatgg accagttcgg tccgccggcc
     1381 caggccctcg agggaagctt ccgccgctgg gccgatcgcc gtggaagaat ttccagaggt
     1441 tcgccctttc cgcgaatttc cctacaacta tctgcgccgc ttgaacgagt ttgcctggga
     1501 cttccggcag cggacgccga cgcgcctgag cttgatgcgc cgcatccgcg ataagatgca
     1561 gccgccgtcg gtccgcgcga tgaaactgcc ggctcgagcg gtcgctctgc tgggagccga
     1621 gcagtggttg gaagatcggc ttgagggcct gctcctcagc tatccgcgga gcccggaggc
     1681 ggtccaacgc ctcaaacaac tgcggccggc tttgctggtc gccaccggcc ctttttggtt
     1741 tctggagccg ggagttgtcg cggctgcgaa aaccctggga attccttgcc tggcgctgat
     1801 tccttcctgg gacaatctct ccacgaagaa ccgcatggtt ttccagtatg acggatacct
     1861 tgtctggtcg gagcaggccc gggaggaatt gcgcgcgttt tacccttcga gccggccagt
     1921 tccggcatat gtcgtcggcg cgcctcaatt cgacgtgttc tttcaacgcc ggttccatca
     1981 atcccgcgag gatttttgcc ggaagcacgg gctgcgtccc ggctgtccga tcatcttata
     2041 tgccgtcggg tctcccaacc tgattcggga gcagcatggc gccgtgtaca tggccgaacg
     2101 cgtggcccgc ggcgaattgg gggaggtgca gcttctcatt cgcccgcatc caatccacga
     2161 caacggggaa atgaaccagc tattcggccg gtttggacct cgcgtggttc tgcaacaaac
     2221 gggcgaggcg gggctcgccg tagccgcccg gcgccaggac gaagatcaga tcgccgcctg
     2281 ggtgaacact ttccggcacg ccgatgtcgt ggtgaatctc tcctctacgg tggctattga
     2341 cgcggcgatc atggaccgcc cggtcgtcaa tctggattac gatccggaac ccggccagcc
     2401 gaatcagggc cttgtgaagg acatcaatca cacctggacc cacttcaagc ctgtagcgga
     2461 atccggcggg gtctggctgg tcaacaatcc ggaagagatg gttcacgcgg tgaagacata
     2521 tctgcgc
//
LOCUS       DASREK010000033        17676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2327316, whole genome shotgun sequence.
ACCESSION   DASREK010000033 DASREK010000000
VERSION     DASREK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17676
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2327316"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /gene="rocF"
                     /locus_tag="VFA54_01985"
     CDS             complement(<1..814)
                     /gene="rocF"
                     /locus_tag="VFA54_01985"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZS49600.1"
                     /translation="MPITKIKAIGVPLDLGQDRRGVDMGPSAIRVANLNARLRSLGYE
                     VEDAGNVDVVIPETLHYGSRHAKYLKPIGETCKRVAREVTKALRAGFAPLVLGGDHSI
                     AIGTMAGVSRYYRDRKQRVGLIWIDAHADMNTPETSPSGNIHGMPLACCVGLGPRELT
                     RLAGYAPLVHASNVALIGVRDVDALEKPHVRCSGVRAFTMRHIDERGLRAVMEEAIAI
                     ALERTAGFHVSLDMDYVDPAEAPGVGTPIRGGGTYREAHLLMEMIADTGRMLS"
     gene            complement(876..2171)
                     /gene="asnS"
                     /locus_tag="VFA54_01990"
     CDS             complement(876..2171)
                     /gene="asnS"
                     /locus_tag="VFA54_01990"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HZS49601.1"
                     /translation="MTIDQAARHEGQIVELHGWLYNLRKSGKILFPIFRDGTGLMQGV
                     VVKTDVSAEVFERVKELTQESSVVVTGRVRGEPRAPGGYELDVADVQVLQKIPVDDPY
                     PITPKEHGIEFLMDHRHLWLRSSRQHAILRVRHAVIRAVRDYFDNHGFTLVDTPILTP
                     AACEGTTTLFEVPYFDEEKAYLTQSGQLYNEATAMAFGKVYCFGPTFRAERSKTRRHL
                     TEFWMVEPEMAFATIDDVMDLAEDFISSVVQRVLQEKKSELKTLERDTTKLQNVKPPF
                     PRISYDEAVERLKKKGSEIQWGGDFGGTDETLLSEDFDRPVMVHRYPAAVKAFYMQPD
                     PHRPEVALGLDVLAPEGYGEIIGGGERIHDLNLLLRRIEEHKLPKEAFNWYLDLRKYG
                     SVPHAGFGMGVERCVAWICGLEHVRETIAFPRMLYRLRP"
     gene            complement(2168..2998)
                     /locus_tag="VFA54_01995"
     CDS             complement(2168..2998)
                     /locus_tag="VFA54_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZS49602.1"
                     /translation="MRHVLSTHVFVQHRLTVALLDRIAKAGISEIEIFCARQHLDYQN
                     RAQIEELAHWFRDSDLKVHSMHSPMYRDTIWGRSGPHSVISITETEKVRRIAATDEIK
                     RALEIAELIPFRYLIQHIGVGQEEYHDRKMDAAFTSLEELSIFAGHRGVALLVENIPN
                     ALSTPERLVAFLNSTHLPIDFVFDTGHAHIMGGVEPAFQMMQARIRSTHVHDNDGEQD
                     SHLFPSKDRPGGIKWKETMHLLRSRQDQYPLLLELREVPDMEHPLNEIRSVFDRLESL
                     "
     gene            complement(3014..4681)
                     /gene="ggt"
                     /locus_tag="VFA54_02000"
     CDS             complement(3014..4681)
                     /gene="ggt"
                     /locus_tag="VFA54_02000"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZS49603.1"
                     /translation="MKKLCAAWLLFACALAGASREPVRARKAMVVTHEDHATQAGLRV
                     LEQGGNAIDAAVAVGFALAVTHPAAGNIGGGGFMLVRLADGRSTFIDFRERAPRRATP
                     GMYLDSVGNVTGDRLFGYRASGVPGTVRGLEYASKKYGRRMWADLLAPAIELAKNGFP
                     VSYDLANSLRSSEALERNSESRRIFQRNGKYFEPGEILIQPELAATLERISKFGSRDF
                     YAGETAQRLAADMARHGGLIGLQDLQQYQVVERRPLDGSYRGYGILTAPPPSSGGAGM
                     IQMLNMLDGSGYEKTGAGSAATIHYIAEVMRRFFADRSKYFGDPDFVKIPLRELLSRH
                     YAEERRRSIDPERATPSAQLGPGDLQGYESGETTHYSILDAEGNAVAVTYTLNARFGS
                     GVTAAGLGFLLNNEMDDFTAKPGVPNMFGLLQSQANAIAPGKRPLSAMTPTIVTRDGK
                     PFLILGSPGGPTIISTVLLVTLNVIDFGMDVQQAVDWPRFHHQWMPDELRLEESGFSP
                     DTVELLRRRGHKLRISGYQGDVAAIECKDGWILGAPDSRTEATARGY"
     gene            4777..5211
                     /locus_tag="VFA54_02005"
     CDS             4777..5211
                     /locus_tag="VFA54_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49604.1"
                     /translation="MLELRVVMESSRAAVEANLSPPARAHTSLTSIVLVFLCTLTGAA
                     AQILMKQGADHTTGAGLAGLLTNLPLFLGYCIYGVNTILVVLALRQGHLSVLYPIIAL
                     SYVWVTMLSPRYFPGDHLNGFKIAGVFMIVAGVSMIGIGSRQ"
     gene            5208..5564
                     /locus_tag="VFA54_02010"
     CDS             5208..5564
                     /locus_tag="VFA54_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZS49605.1"
                     /translation="MTTPLSSILLVFAASVIGSAGMVALKAGANRLPPGLLGILFSWR
                     VAIGVALFLLSSVFYVLGIRHGSLTVLFPMVSLSYVWGVLLARLLFHEPLNRSKIMGL
                     LLLLTGVVFIGLGNRP"
     gene            5768..7174
                     /gene="leuC"
                     /locus_tag="VFA54_02015"
     CDS             5768..7174
                     /gene="leuC"
                     /locus_tag="VFA54_02015"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZS49606.1"
                     /translation="MAEARTLFRKIWDSHVVAEEPGCPAVLYIDLHLVHEVTSPQAFQ
                     ALREKALKVRRPLQTLATMDHSIPTTDPALPILDPVAARQVEQLAINCQEFGVPLYDR
                     KNKHQGIVHVIGPELGATQPGMTIVCGDSHTSTHGALGALAFGIGTTEVEHVLATQCL
                     LQTRPKTFEVRVDGRLKPGVTAKDVILALIAKIGVGGGTGHVFEYTGSAIRSLTIEGR
                     MTVCNMSIEGGARAGLVAPDDATIQYLAGRPFAPKGAAWDAAVAAWRRLPSDEGAAYD
                     QRVEIDANQLEPMITYGTNPGMGVPITAPVPDPASIPEPSHRSTLAKALEYMGLQPGR
                     PLLGHAIDVVFIGSCTNSRISDLRLAAQLLKGRKVNPRLRVMVVPGSQQVKAQAEAEG
                     LPEIFRAAGCEWREPGCSMCIAMNGDELKPGQYCVSTSNRNFEGRQGKGGRTFLASPL
                     TAAASALTGVVTDVRTFF"
     gene            7259..7873
                     /gene="leuD"
                     /locus_tag="VFA54_02020"
     CDS             7259..7873
                     /gene="leuD"
                     /locus_tag="VFA54_02020"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZS49607.1"
                     /translation="MNPITSFSSRIAPLPIDNIDTDQIIPARFLKTTDKSGLGRNLFC
                     DWRYDAEGNPRPDFILNQPRAAGAAVLLAGDNFGCGSSREHAPWALLAFGFRAIISTS
                     FADIFRNNCLKNGLLPIVVDAGTHAELLEIAGSNPAAEVHVDLATQSLRLPSGRTVQF
                     PIDSFSKHCLLNGVDQLGYLLQKEALIAAYERAYPPRITTYTRE"
     gene            7955..8233
                     /locus_tag="VFA54_02025"
     CDS             7955..8233
                     /locus_tag="VFA54_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49608.1"
                     /translation="MHERELNEIHQIAREMVHLAVQSVQDSEEFVCLIEGGGVCLGNS
                     FSEWNAAEKWIRSMFGRLFPDHRCGLGCIRMPHAEFCADDEVLAELAG"
     gene            8329..8769
                     /locus_tag="VFA54_02030"
     CDS             8329..8769
                     /locus_tag="VFA54_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49609.1"
                     /translation="MNSKPTHADAQLLLQLYDLRRERRLRRARDFVQNDCKFKDYKDF
                     EKKYPLGSKERNYVGMVLGYWDMACTLVTNGLLNEDLFNAMNFEHVGVWFKLKPVAQA
                     YREKYHYNAIMQSLETVASRHPGAAMYQSDGTRAASQAKSRRAR"
     gene            8798..9388
                     /locus_tag="VFA54_02035"
     CDS             8798..9388
                     /locus_tag="VFA54_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49610.1"
                     /translation="MISQRLLRLVQQNADRLTNDLIAELQRDPRTSAYHKLSEQDLHE
                     RALDLFHNLGQWLSARTEFAVQTRYEAFGRRRYQENIPFSQALFALTTAKSMLLNFIR
                     GAAAAESAAELPLEYELATAISQFFDKALYHAAAGYEDAARAAARTPLRTPEPVLAHT
                     TPADMVRKPLSREASAESRDLGLEVSRSGDIGESGG"
     gene            complement(9392..9721)
                     /locus_tag="VFA54_02040"
     CDS             complement(9392..9721)
                     /locus_tag="VFA54_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HZS49611.1"
                     /translation="MATVALPEIDDEILDRLEPLYNLILLDDDDHTYDYVVEMLVKIF
                     GFSETVAFRHAVEVDTEGRTLLLTAPLEEVQAKREQIHAYGADWRLERSKGSMSAIIE
                     PAGPSEK"
     gene            10139..12721
                     /locus_tag="VFA54_02045"
     CDS             10139..12721
                     /locus_tag="VFA54_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="interleukin-like EMT inducer domain-containing
                     protein"
                     /protein_id="HZS49612.1"
                     /translation="MISRSFVRGLLAVLFAISGRAQFEIRGSRLYIDGNAFPVHGVSY
                     NPTPIGQTAGAVLQLSGCLYARDFPLMPLAGINTVRLYGRVPAGESVLWRELQDNHLY
                     VLAGFPLDAYYDPQATLSADDDAGRTLRSKIIEDFRQYARQLQNSKLVIALIFGNEVT
                     ANYHTHFAGNPRDFYTLVQEAANMLQSEFGSAAPLLTTAVADNGDIGQPALAARDSDL
                     PGLAFWSVNVFRGQSFRGLFDELRGKTAKPVLIAAFGIDAFDNTLQREDADSQAAAVH
                     SLARELAREMARPDSRALGGVWFSWSDEWWRGGPDASRHGTAGSLQDGFPDGVINPGW
                     FGLFGLVATGQPGLDSLRPRSAFAALAQEWGGALPAAWPAPMPELSPQGVVNAGSLQA
                     TIAPGSLMSLFGQNLAPALTSGSASSPPFHMGLTSACLAGQAIPLLAAGPSQINGQAP
                     WASPTGTAAAVIAYRAGIASNSVTVNVQAFAPGILDRGVLPAGRPCPVSTVNGVRPGA
                     YLEIYGTGLGSARDAPPTGFAPETPVALGNPPRVFLSGQELRVLYSGLVPGVVGLYQT
                     DVQVSADFSPSSSAGLQLLAGGVLSNPYPISVINGLEQPGYTLGGDTLNFLVQAGGPP
                     QTAFLSIDGYNGFCELVRFSVSGLPAGISASLPVGFPGQRIPVSVQAAPQAPGISNAG
                     GVIQGDSLAPQNPSIPLQVSVLPSQGDITFRVTSGGGRAGLVARFEMAGRLLAEVHGG
                     GPGRGFNFLTLDGTTGLLGTPYNFDTWLNEQASEDMADYLASLPPGTVVLGAIADEGT
                     LHLTPRGRAALQHVLHAQTIDLLQYQDSWAIISRVGSSLPFAEDRAGDRQVLLQSVLT
                     FPMP"
     gene            12804..13628
                     /locus_tag="VFA54_02050"
     CDS             12804..13628
                     /locus_tag="VFA54_02050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZS49613.1"
                     /translation="MFPLFRFVPSPILRRLSAIRTIRRAYAALLPGDARIVDHGRAGL
                     KLFIVPRKNKAEWAAGGFKRYEPEQVACFEKLIHPGDCIFDVGANVGFYAIWFSRLAV
                     AGRVVAFEPDPANVELLRRNLELNKVCNVAVEPCAVSDYAGERRFAVDSATGSTGTLE
                     SHQTYGSRLEGGPQMFATVRTTTLDEAAREYGYPALMKIDVEGHELAVLKGARQVLGS
                     PCLRALAVEVSNASTRTSVYALLREHGFQLRDIERNYAEAGERCYALLAARQELTP"
     gene            complement(13760..15406)
                     /gene="ggt"
                     /locus_tag="VFA54_02055"
     CDS             complement(13760..15406)
                     /gene="ggt"
                     /locus_tag="VFA54_02055"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZS49614.1"
                     /translation="MFLFPRAFLHAQGRNYGRSMVISQQGIVATSQTLASQAGAYILA
                     KGGSAVDAAIAANAVLGVMEPMMNGIGGDLFVMYWEAKTGKLTGLNASGPAPAGLSAE
                     FLRKKGFQQMPSEGIHTVTVPGAVRGWAVIHERYGKLPWKDLFGPAIAYAERGFPVAE
                     AIDEAWVSAGSVKKLKAHAESARVFLPGGQPPQPGDVFRNPEMARALHLVANQGPEAF
                     YKGEIAAAILKTSQRLGGTMTAEDLASFSPEWVQPISTDYRGWRIYELPPNGQGMAAL
                     EMLNIMETAPAAPEGPFSANEMHEKIEAMKLAYADMRRYDADPRTYDVPLGVLLSKGY
                     ARKRAALINPKRANCSAPPGEAIGSDTTYLTVVDKEGNIASWIQSLSAAFGCGITVEG
                     MGFLLHNRGEYFTLDPKHPNVLAGGKRPLHTIIPAFMEKGDLHIGFGIMGGFNQPLAH
                     AQFVSNIVDYGMNVQAAMEAPRFTKRSASGCDVTIEVRVPPATLQQLSERGHEIRIAR
                     EYTQTMGRGQAILHNSKTKTNYAASDPRADGSAVPEPMQP"
     gene            complement(15701..15964)
                     /locus_tag="VFA54_02060"
     CDS             complement(15701..15964)
                     /locus_tag="VFA54_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HZS49615.1"
                     /translation="MTVRQHSKAIRDVSPPTRGCQECLQSGDAWVHLRLCLNCGHVGC
                     CDSSKNKHAPKHFHQMRHPIIRSIEPGESWGWCYVDEIMLDFS"
     gene            16084..16719
                     /locus_tag="VFA54_02065"
     CDS             16084..16719
                     /locus_tag="VFA54_02065"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZS49616.1"
                     /translation="MSTELIEFDCENPDSGVVQYAAKAIQQGKVVAIPTDALYVLAAD
                     PFNLRAVGQVFQAKGREIHRSLPLLVDSVLMAEDLSTNLSSRFYLLARHFWPGPLTLI
                     VPASAKVPLKVTGNTGRLAVRQARCAVANRIIELLEMPIIATSANISGQPTCRSSIEV
                     FGFMDNRVDLILDGGTVGGAGATTVDITEPYWRMIREGSISEAEIAECLKG"
     gene            complement(16834..>17676)
                     /locus_tag="VFA54_02070"
     CDS             complement(16834..>17676)
                     /locus_tag="VFA54_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49617.1"
                     /translation="GVRYVDAQSNLQMNWSPINGGTAGQILNRMNGRTASTLYMGTMG
                     TNTYDGLQTRLQGHFAGGYQVGLTYSFSKALGYATSAPVQIPQYWSLNRGPQATDITH
                     SLSAVSVAELPFGKGKRWAQSGVSSKLAGGWQISGLLVAHSGPPFTAGASNSTLNAPY
                     SSQFADCLSAPQKLGSILQWYSRSAFAVPASGRFGTCGVNSLRGPGLFNADLSVQRKF
                     PITEQLQLTFRAEMFNVANTPHHAMSSTSVSSGTFLQALNIANTGREGTEQRAVRLAL
                     RLSW"
BASE COUNT         3359 a         5388 c         5322 g         3607 t
ORIGIN      
        1 tcgagagcat gcgcccggta tcggcgatca tttccatcag gaggtgcgct tcgcggtacg
       61 tgccgccgcc ccggatcggc gtgccgacgc cgggagcctc ggcggggtcc acgtaatcca
      121 tatccagact gacgtggaat ccagccgtcc gttccaaggc gatcgcaatg gcctcctcca
      181 tcaccgcacg cagtccgcgc tcatcgatgt ggcgcattgt gaacgcgcga actcccgagc
      241 agcgcacgtg cggcttctca agggcgtcca cgtcgcgcac tccgatcaga gcgacgttgg
      301 aagcgtggac cagcggggca taaccggcca agcgggtcag ctcgcgaggg cccagcccca
      361 cgcagcacgc caggggcatg ccgtggatat tcccgctcgg gctggtttcc ggagtattca
      421 tgtcggcgtg ggcgtcaatc cagatcaggc ccacgcgttg cttgcgatcc cggtaatacc
      481 ggctcacgcc ggccatcgtg ccgatagcga tcgaatgatc tccgccgagt acgagcggcg
      541 caaatccggc gcgcagggct ttcgtgacct cgcgcgccac gcgcttgcag gtttcgccaa
      601 tcggcttcag gtacttggca tgacggctgc cgtaatgcag cgtctcggga atcaccacgt
      661 cgacgttgcc ggcatcctcg acttcgtagc ccagggagcg caggcgcgcg ttgaggttgg
      721 ccacccggat cgcagacggt cccatatcca ccccgcgccg atcctgtccg agatccaggg
      781 gcactccaat ggccttgatc ttagtgattg gcattgatcg cgcgccgggg gcccgattgc
      841 gagcggaagt tggcggccat atttttatga taaatctacg gccggagccg gtatagcatc
      901 ctggggaagg cgattgtctc ccgcacgtgc tcgagaccgc agatccacgc cacacagcgc
      961 tcgacgccca tgccgaagcc ggcgtgcggg acgctcccgt atttacgcag atcgagatac
     1021 cagttgaacg cctctttcgg gagcttgtgt tcttcgatgc ggcgaagcag caggttcaga
     1081 tcatgaatgc gctctccccc gccgatgatc tccccgtatc cctcgggtgc gagcacgtcc
     1141 aagcccaagg ccacctccgg cctgtgcgga tcgggctgca tgtagaacgc tttcactgcg
     1201 gccgggtagc ggtgaaccat gacgggccgg tcgaagtctt cgctgagcag agtttcgtcc
     1261 gtgccgccga aatccccgcc ccactggatc tcgcttcctt ttttcttgag ccgttccacg
     1321 gcttcgtcat agctgatgcg tggaaacggc ggcttgacgt tttggagctt cgtcgtgtcc
     1381 cgctcgagcg tcttcagctc ggatttcttt tcctgaagaa cgcgctgcac caccgagctg
     1441 ataaagtcct cggcaagatc cataacatca tcgatggttg caaacgccat ttccggttcg
     1501 accatccaga attcggtcag gtggcggcgc gtcttcgacc gctcggcgcg gaaagtcgga
     1561 ccgaaacagt agactttgcc gaaggccatc gccgtggctt cgttgtaaag ctggccggat
     1621 tgcgtgagat aggccttttc ctcgtcaaag taaggcacct cgaacagggt tgtggtgccc
     1681 tcgcaagcgg cgggcgtgag aatgggggtg tcaaccagag tgaatccgtg gttgtcgaaa
     1741 taatcgcgca cggcgcggat caccgcgtgg cgcactcgca ggattgcgtg ctggcgcgag
     1801 ctgcgcagcc aaagatggcg gtgatccatc aggaactcga tgccgtgttc cttgggggtg
     1861 atcggatacg gatcgtccac gggaattttt tgcagcactt gcacatcggc aacgtcaagc
     1921 tcgtagccgc cgggagcgcg cggctccccc cgcaccctgc ccgtcaccac caccgaagac
     1981 tcctgcgtca actccttcac gcgctcgaag acttctgccg acacgtccgt cttgaccacc
     2041 actccctgca tcaggccggt gccgtcgcga aagatcggga acagaatttt gccggatttg
     2101 cgcagattat aaagccagcc gtgaagttct acgatctggc cctcatggcg ggcggcctgg
     2161 tcaatggtca cagactctcc aaacgatcga acaccgagcg gatctcattg agcggatgct
     2221 ccatatcggg aacttcccgc aattccagca gcaaagggta ttgatcctgg cgggatcgca
     2281 gcagatgcat cgtttctttc catttgatgc cgccgggccg gtccttgctg gggaacaaat
     2341 gcgagtcctg ctctccatcg ttgtcgtgga catgcgtgga acgaatccgc gcctgcatca
     2401 tttgaaaggc cggctcgacg cctcccatga tgtgagcgtg cccggtgtcg aagacaaaat
     2461 cgatcggcag atgcgtgctg ttgaggaacg cgaccagccg ttccggcgta gaaagcgcat
     2521 tcgggatgtt ttcgacgagc agggccacgc cccggtggcc ggcgaagatg gatagctctt
     2581 cgagactcgt gaaggcagcg tccatcttcc ggtcgtgata ctcctcttgc ccgacgccaa
     2641 tgtgctggat gaggtagcgg aacgggatca gttccgcgat ttccaaagcc cgcttgattt
     2701 catccgtcgc cgcgatccgg cgcacctttt ctgtttccgt tatcgaaatg accgaatgag
     2761 gcccgctgcg gccccagatg gtatcacgat acattgggga gtgcatcgaa tgtactttca
     2821 aatccgagtc gcgaaaccag tgggctagtt cctcaatctg cgcgcgattt tgatagtcca
     2881 ggtgctgccg cgcgcagaat atctcaattt cggagatacc tgctttggcg atccggtcga
     2941 gcaacgccac agtcaggcgg tgctggacaa agacatgggt ggagagaacg tggcgcattt
     3001 tcaaaaatcc aaatcaatag ccgcgagcgg tggcttcggt gcgagagtca ggcgctccca
     3061 ggatccatcc gtctttgcat tcgatggccg ctacgtcgcc ctggtagccg gagatgcgca
     3121 gcttgtggcc gcgccgccgc agcagttcca ctgtgtcggg cgaaaagccg ctctcctcga
     3181 ggcgcaattc gtcaggcatc cactgatggt ggaaccgggg ccaatcaacc gcctgctgaa
     3241 catccattcc gaaatcgatg acgttcagag tcaccaacag cacggtggaa ataatggtgg
     3301 gcccgcccgg tgaacccaag atcagaaatg gcttgccatc ccgcgtgacg atagtgggcg
     3361 tcatggccga tagcggacgc ttgccgggcg caatggcgtt cgcctggctc tgcaacaggc
     3421 cgaacatgtt ggggacgccc ggctttgccg tgaaatcgtc catctcgtta tttaggagga
     3481 agccaagacc cgccgccgtg acgccggaac cgaaacgcgc gttgagcgta taggtgaccg
     3541 cgaccgcatt tccttcggcg tccagaatgg aatagtgggt cgtttccccg ctttcatatc
     3601 cctgcagatc ccccggcccg agctgcgcgc tgggcgtggc cctttcggga tcgatgctgc
     3661 ggcgccgctc ctcagcgtaa tgccgcgaga gtaactctct caggggaatc ttcacaaaat
     3721 cggggtcgcc gaaatatttc gagcggtcgg cgaaaaagcg gcgcatgact tcggctatgt
     3781 aatggattgt cgctgcggac cccgcgccgg ttttttcgta accgctgccg tccaacatat
     3841 tcagcatctg aatcatgccg gccccgccgg aactcggcgg cggcgcggtg agaatcccgt
     3901 agccgcggta ggatccatcc agtggcctgc gctcgacgac ctggtactgc tggaggtcct
     3961 gcaaaccgat cagaccaccg tgccgcgcca tatccgccgc cagccgctgc gcggtctcgc
     4021 cggcgtagaa atcgcgcgag ccgaatttgg aaattctctc cagggtggcc gccagctccg
     4081 gctggatcaa tatttctccc ggctcaaagt atttcccatt gcgctggaag atgcggcgcg
     4141 actccgaatt ccgctccaat gcctcgctgg aacggagcga attcgcaaga tcatagctga
     4201 ctggaaaacc gttcttcgcc aattcgattg ccggcgccag caggtccgcc cacatccgcc
     4261 gcccatattt cttcgatgcg tattccaacc cgcggacggt tccaggaacc cccgaggccc
     4321 ggtaaccgaa caggcggtcg ccggtcacat ttcctacgct gtccaggtac atgccgggag
     4381 tggctctgcg cggggcccgc tcgcgaaaat cgatgaatgt ggagcggccg tcagccagcc
     4441 gcaccagcat gaaaccgccg ccgccaatgt ttcccgcggc tggatgggtg acagccagcg
     4501 caaagccaac ggcgacggcc gcgtcaatgg cgttgccgcc ttgctcgaga acgcgcagcc
     4561 cggcctgggt cgcgtggtcc tcatgtgtca cgaccatcgc tttgcgcgca cgcaccggtt
     4621 cccggctggc gccggcgagg gcgcaggcga agagcagcca ggcggcgcaa agcttcttca
     4681 tacgaaacaa attgtaccaa ttcgaggggg ccctccccga ccgaaggtcc ccgccgcgcc
     4741 gccacagccg aaggggacgg ggccagaatt tcaggcatgt tagaattgcg ggttgtgatg
     4801 gaatcgagcc gcgcggctgt cgaagcgaac ctatccccgc ccgcccgggc gcacaccagc
     4861 ctgacctcga tcgtcctggt gtttctctgc acactgacgg gcgccgcggc ccagatcttg
     4921 atgaagcagg gggccgatca tacaaccgga gccggtttag ccggacttct gaccaacctg
     4981 ccattgtttc tcggttactg catctacggc gtaaacacga ttctggtcgt gctggcgctg
     5041 cgccaggggc acctgtcagt gctttacccc attatcgctt tgtcttatgt ctgggtgaca
     5101 atgctgtcgc cgagatactt tcccggcgat cacctgaacg gctttaaaat cgccggcgtc
     5161 ttcatgattg tggcaggtgt ttccatgatc ggaatcggga gccgccagtg accactcccc
     5221 tttcttccat actgctcgta ttcgccgcca gcgtcatcgg ctcagccgga atggtggcct
     5281 tgaaagcagg cgcaaaccgc ttgcctccgg gcttgttggg gatcttattt agttggcgcg
     5341 tagcgatagg cgtggcgctg ttccttcttt cctccgtctt ttacgtgctc gggatccggc
     5401 acggcagcct cacggtgctg ttcccgatgg tttctttgag ttacgtctgg ggcgttctgc
     5461 tggcccgcct gctattccat gagccgctga accggtccaa gatcatggga ttgctgctgc
     5521 tgctgaccgg tgtggtcttc atcggccttg ggaatcgtcc ttagccggtc gattttcggc
     5581 ggaaacagaa tttccgcctt tgtgtacaag gtcgccggac aaaagaaatt agccgcggcg
     5641 gtctcccgcg gcaaatggcg aacggcgttc ctcttgcgca agaagcgctt aagatcacgg
     5701 gcagacagcg actcgaggtc tatggatcgc ctgcttctgc gggctggaat tgataaaatc
     5761 tattttgatg gccgaagcgc gcactctctt tcgaaaaatc tgggattccc acgtagtagc
     5821 tgaagagccg ggatgtccgg cggttcttta catcgatttg catctggtcc atgaagtcac
     5881 ctcgccgcaa gcgtttcagg cactgcgcga aaaagcctta aaggttcgcc ggccgcttca
     5941 aacgctggcc actatggatc acagtattcc gacgaccgat ccggcgttgc cgatactgga
     6001 cccggtggcg gccaggcaag tcgagcagct tgccatcaac tgccaggaat tcggagtgcc
     6061 gctctacgac cggaagaaca agcaccaggg cattgttcac gtgatcggcc ccgaactggg
     6121 cgccacgcag cccggaatga ccattgtttg cggcgacagc catacctcga cgcacggcgc
     6181 tctgggcgcg ctggccttcg gcatcggaac caccgaagtg gagcacgtgc tggccacgca
     6241 gtgtctgctg caaacgcggc ccaagacgtt tgaggtccgc gtggacggca ggctgaagcc
     6301 cggggtgacg gcgaaggacg tcattctggc cctgatcgcc aaaatcggcg tcggcggagg
     6361 tacgggacac gttttcgaat acaccggcag cgcgatccgg tcgctcacca tcgaaggccg
     6421 catgacggtc tgcaacatgt ctatcgaagg gggcgcgcgc gcgggactgg tggcgcctga
     6481 cgatgccacg atccagtacc ttgccggccg gccctttgcg ccgaaaggcg cggcttggga
     6541 cgcggcggtg gccgcgtggc gcaggctgcc cagcgatgag ggggccgcct acgatcagag
     6601 agtggagatc gacgcgaatc aactcgagcc catgatcacg tacggcacca atccggggat
     6661 gggcgttccc attacggccc ccgtgcccga cccggcatcg attcccgagc cgtctcatcg
     6721 ctcgactctg gctaaagcac tggagtacat ggggcttcag ccagggcgtc cgctgctggg
     6781 ccacgccatt gacgttgtat ttattggaag ctgcacaaac tctcgtattt ccgatttgcg
     6841 gctggccgcg caattgctaa agggacgcaa agttaacccg aggcttcggg ttatggtcgt
     6901 gcccggctcg cagcaagtca aggcgcaagc cgaggccgaa gggctgcctg agatctttcg
     6961 ggctgccggc tgcgagtggc gggagccggg ttgcagcatg tgcatagcaa tgaatgggga
     7021 tgagctgaag ccgggccagt actgcgtctc gacatcgaac cggaactttg aaggcaggca
     7081 ggggaaaggc gggcgtactt tcctcgccag tccccttacc gccgccgcca gcgctctcac
     7141 aggggtggtt acagacgtga gaacgttctt ctagatgtgt cttccacgct tccggcgacc
     7201 gtcccacggg ctggaagcga gacgcctact gaagagggat ctggagatct cgagaattat
     7261 gaacccgata acgtcttttt cctcgcggat tgcgccgttg cccattgaca acatcgacac
     7321 cgaccagatc attcccgccc gcttcctgaa aaccaccgat aaatccggcc ttggcaggaa
     7381 tctcttttgt gactggcgct acgatgcgga gggaaacccc cggcccgact tcatcttgaa
     7441 tcagccgcgc gccgccggag ccgccgtctt gctggccggc gataactttg gatgtggcag
     7501 ttcgcgggag cacgccccct gggcgttgct ggcatttggg ttccgcgcca ttatcagcac
     7561 atcgttcgcc gatatattcc ggaacaactg cctgaaaaac ggcctgctcc cgatcgttgt
     7621 ggacgctggc acgcacgccg agcttttgga aatcgccggg agcaacccgg cagccgaggt
     7681 tcatgtggat ttagccactc agtctcttcg cctccccagc ggccgcaccg tccagtttcc
     7741 gatcgacagc ttctctaagc actgcctgct caacggcgtc gatcaactcg ggtatttgct
     7801 ccagaaagag gcgctgatag cggcatacga gcgggcttat ccacctcgta tcacgacata
     7861 tacacgggaa taggaaacga ggtaccgacg tactcaccca aattgaatcc ggggagctat
     7921 tggttcaatt ctgggtctcg ggcaagatcg gtgtatgcat gagcgcgagt tgaacgaaat
     7981 ccaccagata gcccgcgaga tggtgcacct ggcggtgcaa tccgtccagg actcggaaga
     8041 gtttgtgtgc ctcatcgaag gcgggggagt ttgtctgggc aactcgtttt ccgaatggaa
     8101 tgcggcggag aagtggattc gcagcatgtt tgggcggctg ttccccgatc accgctgcgg
     8161 cctcggctgc atccgcatgc cgcacgccga gttctgcgcc gacgacgaag tccttgccga
     8221 gttggcaggc tgaagcggcg ttcctgaaag atccccgatc gaaggctctg cctgtttccc
     8281 cgttctgggg tatcatagtc ccatcgccaa agtgccggag gaccagcggt gaactccaaa
     8341 cccacacacg cggacgccca gcttttgctc caactctacg acctgcgccg cgagcggaga
     8401 ttgcggcgcg cccgcgactt cgttcagaac gactgtaagt ttaaagatta caaagatttc
     8461 gaaaagaaat atccgctggg aagcaaagag agaaactacg tcggcatggt gctgggctac
     8521 tgggacatgg cttgcacgct ggtcacgaac ggcctgctga acgaggactt gttcaacgcc
     8581 atgaacttcg agcacgtggg cgtatggttc aaactgaaac cggtggcgca agcttaccgc
     8641 gagaagtacc attacaacgc catcatgcaa tcgctcgaaa ctgtagccag ccgtcatccg
     8701 ggtgcggcta tgtaccaatc tgacgggacg agagctgcat cccaggccaa gagccggcgg
     8761 gcccgataag ttcgggagta ggaaaacggg ggaatttatg atttcgcaga ggctgctccg
     8821 gttggtgcag cagaacgcgg accggcttac caacgacctg atcgcggaat tgcaacgtga
     8881 cccgcgaaca tcggcgtatc acaaactatc cgaacaggat ttgcacgagc gggcgctcga
     8941 cctgtttcac aacctcggcc agtggctgag cgcgcgcacc gagttcgccg tgcagactcg
     9001 gtacgaagcc tttgggagac ggcgttatca ggagaacatc cccttcagcc aggctctgtt
     9061 cgctcttacg accgcgaaaa gcatgctgct gaatttcatc cggggcgcgg ccgcggcgga
     9121 gtcggcggcg gaactgccgc tcgaatacga gttggccact gcgatttccc aattttttga
     9181 caaagctctc taccatgcgg cagcaggtta tgaggacgcg gcccgggccg ccgctcgcac
     9241 cccgctgcgg acgccggagc cggtgctggc gcacaccacc cctgccgata tggttcggaa
     9301 gcctctcagc cgggaagcga gcgccgagag ccgtgatctt gggctggagg tgagccgctc
     9361 cggggatatc ggcgaatccg gcggctgatt gctacttttc ggaagggcct gccggttcga
     9421 tgatggcgga catcgaaccc ttgctgcgtt ccagccgcca atccgcgccg tacgcatgaa
     9481 tctgttccct cttggcctgc acctcttcga gcggggcggt caacaggagg gtcctgcctt
     9541 ccgtatccac ctccacggcg tgacgaaaag ccactgtttc gctgaaccca aaaatcttta
     9601 cgagcatctc gaccacataa tcgtaggtgt gatcgtcgtc gtccaggagg atcaaattgt
     9661 agaggggctc gaggcggtcg agtatctcgt catcaatttc cggcaatgcc accgtggcca
     9721 tgctcttatg ctaacaagtc ttcagcgcaa cttaagttcg gtggtaacgg gcgtggcaga
     9781 gccgcgccaa ccggcttgct agcgccgtat ccgccgcttg ccgccacaag acttttgttc
     9841 gtcatccggc cttctggctc cccgcccgcc gattacaaaa ccgcagcgtc aaaactcggc
     9901 ttgacagcgt ttacctccgg ggcctgtctt ttccgggatt agaaaggccg cggcagcggc
     9961 gcggagcgag atcacgcagt tgtccggacc cgttgaggct cggtcgttcg cgttctgata
    10021 gccgcctcca agcgggcggg cgccgcggtg cgaaattggc cttcgaaaac atcgcgcatt
    10081 ttccacaatt ccgccttgct ttttcaagaa caccgatata cattattttt cacgcaaaat
    10141 gatttcccgc agcttcgtgc ggggcctcct ggcagtcctg tttgccattt caggcagagc
    10201 ccagttcgag attcgaggtt cccggcttta tattgatgga aacgcctttc cggtgcacgg
    10261 tgtttcttac aacccgacgc cgatcgggca gaccgccgga gccgtcttgc agctttccgg
    10321 ctgcctctac gctagggatt ttcctttaat gccgctcgcc ggcatcaaca ccgtccggct
    10381 gtacggccgt gtaccggccg gtgaaagcgt actgtggcgg gagttgcaag acaatcatct
    10441 ttacgtgctg gcggggtttc ctctggatgc ttattacgat ccacaagcca cgctttcagc
    10501 ggacgacgac gccggccgaa ccctgcgctc caaaattatt gaagatttcc ggcagtatgc
    10561 ccggcaatta caaaattcaa aactggtaat tgccttgatt ttcggcaacg aagtaactgc
    10621 taattaccat acgcatttcg ccggcaaccc gcgcgatttt tacacactag ttcaggaggc
    10681 ggcaaacatg cttcagtccg agtttggttc tgcggcgccg ctcctgacaa cggcggtcgc
    10741 cgataacggc gatatcgggc aacctgcttt ggcggcacgc gacagcgacc tgcccgggct
    10801 cgccttttgg tccgtcaatg tgtttcgggg ccagagcttc cggggactct tcgatgaatt
    10861 gcgcggcaaa accgccaagc cggttcttat tgccgcgttt ggaatcgatg cctttgacaa
    10921 cacgctgcaa cgggaggacg ccgatagcca ggctgctgcc gtgcacagcc tggctcgtga
    10981 actggcccgc gagatggcgc ggcctgattc ccgggcgttg ggcggtgttt ggttcagttg
    11041 gagcgatgag tggtggcgag gcggtcccga cgcttctcgc catggcacgg ctggtagcct
    11101 gcaagacggt tttccagatg gggtaatcaa ccccgggtgg ttcggcttat tcggcttggt
    11161 agcaacaggc cagcctggtc tggattccct acgcccccgc tctgcgttcg cggctctggc
    11221 tcaggagtgg ggaggcgcct tgcccgcggc ctggccggct ccgatgcctg agctcagccc
    11281 gcagggtgtg gtcaatgcgg ggagtctgca agccacgatc gctccgggca gcctgatgtc
    11341 gctgttcggc caaaacctgg ctccggcgct tactagcggc tctgcatcgt cgccgccctt
    11401 tcatatggga cttacatcgg catgcctggc gggtcaggca atcccgctac tcgctgcggg
    11461 gccgtcgcaa atcaatggac aggcgccgtg ggcttcgccc acaggcaccg cagcggcggt
    11521 catcgcctac cgcgccggca tcgcgagcaa ttcagtaaca gtgaacgtgc aggcgtttgc
    11581 gccgggaatc ctggaccgcg gcgtcctccc ggcgggaagg ccctgtccag tcagcacggt
    11641 gaacggagtg cgtcccggcg cgtacctgga aatctacgga accggattag gcagtgcgag
    11701 ggacgcgccg ccgaccggct ttgccccgga gactcccgtg gcgctcggta atccgcctcg
    11761 ggttttcctg agtggccagg aattgcgcgt gctttacagc ggcttggtcc cgggcgtcgt
    11821 cggcctatac caaacggacg tccaggtgtc ggctgatttt tctccctcct cctcggcggg
    11881 cttgcagctt ctagcgggcg gagtgctgag caatccgtat ccgatcagcg tgatcaacgg
    11941 cctggagcag cccggctata cgctcggcgg agacaccctg aattttctgg tgcaggccgg
    12001 gggacccccg cagacggcgt ttctttctat cgacggctac aacggctttt gcgagttggt
    12061 tcgattttcg gtgtccggat tgccggcggg gatctcggcg tcgctgccgg tgggctttcc
    12121 aggacagcgc attccagtga gcgtgcaagc ggcgccgcaa gcgccgggaa tctcgaacgc
    12181 cgggggcgtc attcagggtg attcactcgc cccgcaaaat ccgtcaattc ccttgcaagt
    12241 ttccgtgctg ccgagccaag gcgacatcac gtttcgcgtg acctcgggcg gcggccgcgc
    12301 gggccttgtt gcgcgtttcg agatggccgg acgcctgctc gcggaagttc acgggggcgg
    12361 cccgggccgc ggtttcaatt ttctgacgct cgacggaaca acgggcttgt tgggaacgcc
    12421 atacaacttc gatacttggc tgaacgagca ggcctctgaa gacatggccg attaccttgc
    12481 ttctctgccg cccggaaccg tagtgctggg ggctatcgcc gacgaaggga cgctccacct
    12541 taccccgcgc ggccgcgctg ctttgcaaca cgttttgcac gcccaaacca tcgacttgct
    12601 gcaatatcag gattcctggg ccatcatttc ccgtgtggga tcgtcactgc cgtttgccga
    12661 ggatcgcgcc ggagaccggc aggtcctcct gcaaagcgtg ttgacgttcc ccatgccata
    12721 agccttcggc tgcctcgcac ggaagtcacc cgacacgcgc agttcccact gcgaggttat
    12781 agaatggcct tggccaggat cgcgtgtttc cactgttccg atttgttccg tctccgattc
    12841 tccgaaggct cagcgcgatt cggacgatcc ggcgggccta tgcggctttg ctgccaggcg
    12901 atgcccgaat cgtcgatcat gggcgggcgg gattgaagct tttcattgtt ccgagaaaga
    12961 acaaggccga atgggctgcc gggggattca aaagatatga acccgagcaa gtggcttgct
    13021 tcgaaaagct gatccatccc ggcgactgca tcttcgacgt cggcgccaac gttggattct
    13081 acgccatttg gttcagccga cttgccgtcg cgggccgtgt cgttgccttc gaacccgacc
    13141 cggccaatgt agaactgctg cgcagaaatt tggaactcaa taaggtttgc aatgtggccg
    13201 tcgagccttg cgcggtttcc gactacgcag gagaacgccg ctttgctgtg gattcagcca
    13261 ccggaagcac cggaaccctg gaatcccacc aaacatacgg ctcacgcctg gagggcggcc
    13321 cacagatgtt cgccacagtg cggaccacga ccttggacga ggctgcacgc gagtacggct
    13381 atcctgcctt gatgaagatt gacgtggaag gccacgagct cgccgtgctg aagggggccc
    13441 ggcaggtgct cggctcgccc tgtttgcgag ctctggctgt cgaagtatcg aacgcttcta
    13501 ccaggaccag cgtctacgcc ctattgcggg aacacggctt tcagctgcgc gatatcgaga
    13561 gaaattatgc ggaagctggc gagcgttgct acgcgcttct ggccgcccgg caagaactaa
    13621 cgccctaacc ggcacggcga agccccagcc agcgcggaat ttaggaccgc cgccggcgcc
    13681 caaccccgct ctcgccgcgc ttgcggcggc gccggctggg gcgtcacgcc gcaaagcctt
    13741 cgggctcatt cctgccaggc taaggttgca ttggctccgg aacggccgat ccatcggctc
    13801 gcgggtcgga agccgcatag ttggtcttgg tcttcgaatt gtgcaaaatg gcctggcccc
    13861 gccccatggt ctgcgtgtat tcccgcgcga tccgaatttc atgtccgcgc tcggagagct
    13921 gttggagcgt ggccggaggg acgcgaacct cgatagtcac gtcgcatccg ctggcactgc
    13981 gcttggtgaa tcgcggcgcc tccatagcgg cctgcacgtt cattccataa tccacaatat
    14041 tggaaacaaa ttgcgcatgg gcgaggggct ggttgaagcc gcccataatg ccaaagccga
    14101 tgtgcagatc gcccttttcc ataaaagcgg gaataatggt gtgcagcggg cgcttgccgc
    14161 cggccagcac gttcggatgt ttggggtcga gagtgaaata ttcgccgcga ttgtgcagta
    14221 gaaagcccat gccttccaca gtgatgccgc atccaaatgc agccgagagg ctttgaatcc
    14281 aactggcgat gttgccttcc ttgtccacga cggtcaagta cgtcgtatcg ctgccgattg
    14341 cttctccggg cggcgccgaa caattcgccc ttttcggatt gatcagggcg gcgcgtttgc
    14401 gcgcatagcc cttcgaaaga aggacgccga gcggcacatc ataagtgcgc gggtcggcgt
    14461 cgtagcgccg catgtcggcg taagcgagct tcatggcttc gatcttttcg tgcatctcat
    14521 tcgcactgaa gggcccttcc ggcgccgcgg gggctgtctc cataatgttc agcatctcaa
    14581 gagcggccat gccctgcccg ttgggcggca gctcgtaaat gcgccagccc cggtagtcag
    14641 tggaaatcgg ctggacccat tccggtgaaa acgaggccag atcttcggcc gtcatcgtcc
    14701 cgcccagccg ctgtgaggtt ttaagaatcg ccgccgcaat ttcgcccttg tagaaagcct
    14761 cagggccttg gttcgccacc agatgtaaag cgcgcgccat ctccggattc cggaagacat
    14821 cacccggttg gggcggctgg cctcccggca gaaatacgcg cgccgattcg gcgtgcgcct
    14881 ttaatttttt gacagagccg gcggaaaccc acgcttcatc gatcgcctcg gcgacaggaa
    14941 agccccgctc cgcataagcg atcgccggcc cgaaaagatc cttccacggc agtttgccgt
    15001 agcgctcatg gatcaccgcc catcctctaa ctgcgccagg caccgtgacg gtatgaattc
    15061 cttcactggg catctgttgg aagcccttct ttcgcaaaaa ctcagccgag aggccggccg
    15121 gggccgggcc ggaggcgttc aaaccggtca gcttgcctgt cttcgcctcc caatacatca
    15181 caaaaaggtc gcccccgatg ccgttcatca tgggctccat aactcccaag acggcgttgg
    15241 cggcgattgc ggcatccacg gccgacccgc ctttggccag gatgtatgct cccgcttggg
    15301 aagcgagcgt ttggctggtg gcgacgattc cctgttgcga gatcaccatg gaccgcccgt
    15361 aattgcggcc ttgggcgtgg aggaacgccc gtggaaaaag aaacatgaca gcaaaaagtg
    15421 gggttgcaca gcgaacagcc ttccctgcga ccttcagcat tctttttttc ggcctcctgg
    15481 ggatcgcgta aatgatagca ggcagaaatg gcttggaccg cagcggcggt ctacaaaaaa
    15541 caaccaagtg cggcgtatgc cggtttggag cctcaagagc gcggatgatc ggcgcgcgga
    15601 gctgcggtta gaaaggccat cgcccgctga gaagccggca caggctccgc cggccgccgc
    15661 ccggcattac gccatcggcg ttccgggccg cgcatttcgt ttaggagaaa tccagcatga
    15721 tttcgtccac gtagcaccat ccccaagact ctcccggctc aatcgaccgg ataatcggat
    15781 gccgcatttg gtgaaaatgt ttgggggcat gtttattttt ggatgaatcg cagcaaccca
    15841 catggccaca gttcaggcag aggcgcaaat gaacccacgc gtcgcctgac tgaaggcact
    15901 cctggcagcc gcgagtgggg gggcttacgt cccgaattgc tttcgaatgc tggcgcacgg
    15961 tcataccgaa ttctcctttt gccgggtttc ggggaccatc aagtccagca tacgcgcttt
    16021 cctggcgctc ttgcgcgccc gcgattccgg agcttgcctg tgatatcctt tcgctgagcg
    16081 cccttgtcaa ccgagctgat cgagtttgac tgcgaaaatc ccgactctgg cgtagttcaa
    16141 tacgccgcga aggccatcca gcagggaaag gtagtagcga ttcctaccga cgccctgtat
    16201 gtgctggcgg ccgatccgtt caatctccgg gcggtgggac aggtatttca agccaagggg
    16261 cgcgagatcc atcgctcgct gcctttgctg gtggactccg tacttatggc cgaggacttg
    16321 agcaccaacc tttcctcgcg cttttacctg ctcgcccggc acttctggcc cggaccgctc
    16381 acgcttatcg tgccggcatc ggccaaggtt ccgctcaagg taaccggcaa taccggccgg
    16441 ctggcggtgc ggcaggctcg gtgcgcggtc gccaaccgga tcattgaatt gcttgagatg
    16501 ccgatcatcg ctaccagcgc caacatctcc ggccagccca cgtgccgcag cagcatcgag
    16561 gttttcggct tcatggataa ccgggtagac ctgattctcg acggcggcac ggtgggcgga
    16621 gccggcgcca ccaccgtgga tattaccgag ccatactggc gcatgattcg ggaaggctcg
    16681 atcagcgaag ccgaaatcgc cgagtgcttg aaagggtagc cttctcccct ttctcccggc
    16741 cgaagcccgc agacatcccc aaggatcacc gacactgcgc ccccgctcgg cgatgccggc
    16801 ccgacgctac tccccggttg gaaggcgaga gcgctaccag ctcaacctca gcgcgagccg
    16861 gacagcacgt tgttcagtac cctcgcgtcc ggtattggcg atgttgagcg cctgcaaaaa
    16921 agtgccgctg ctcaccgaag tgctggacat cgcgtgatgc ggcgtgtttg ccacgttgaa
    16981 catttcagcc cggaatgtca actgaagctg ctccgttatg gggaattttc gctgaacact
    17041 cagatctgca ttgaacagac ccgggccgcg caggctgttg actccgcaag tgccaaaccg
    17101 cccggaggca ggcacagcga aggccgatct cgaataccac tgtaggatgc tgccgagttt
    17161 ttggggcgca ctcaggcagt cggcgaactg gcttgaatat ggagcattca gggtcgagtt
    17221 ggatgctccg gccgtgaacg gcgggccgct gtgagccacc agcagtccgc tgatctgcca
    17281 tcccccggcc agcttggaag aaacgccgct ctgggcccag cgcttgccct tgccaaaagg
    17341 cagttcggcg acactgacgg cggaaagaga gtgcgtgatg tcagtagcct gagggccgcg
    17401 gttcagactc cagtactgag gaatctgcac gggagcggag gttgcatagc ccagagcctt
    17461 gctgaaagag taggtgaggc ctacctgata cccgccggca aagtggccct ggaggcgggt
    17521 ttgcaatccg tcgtacgtgt tggtccccat cgtgcccatg tacagggtgg atgccgttct
    17581 tccgttcatc cggttaagga tctgcccggc tgtgcccccg ttgatcggcg accagttcat
    17641 ctggaggttg ctctgggcgt caacgtaacg cactcc
//
LOCUS       DASREK010000034        25884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_845223, whole genome shotgun sequence.
ACCESSION   DASREK010000034 DASREK010000000
VERSION     DASREK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 25884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25884
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845223"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1470)
                     /gene="larA"
                     /locus_tag="VFA54_02075"
     CDS             complement(250..1470)
                     /gene="larA"
                     /locus_tag="VFA54_02075"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HZS49618.1"
                     /translation="MRKQLPYGRATLSVSFPEDLSVEWVQPDYGPGVPDPSALLAECL
                     RNPIGSRPLADQARGAKRVAIVVDDITRPTPSRLMLPAVISELHAAGVAAEGIRIVVG
                     VGSHRTMTPAEMKEIVGSDIFARYTVMNHQARDARHLADIGRSPRGIPASINRAIVES
                     DLAVLTGFIKPHNVAGYSGGYKAYFPAVSGMDTIVGLHDLQQLPGEPCRLGEIDNPFR
                     TEVDAMGPMVPAPTFLLNVMINRRKQIVRAFAGDPMEAHRAAVAETAKRATVPVSRPA
                     DVVLACAGGYPSDISLYQGINALTSVVRLRKPLVKEDGHVILLGEFREGLGSEFGGSG
                     RHKDLATAWASHLKKMRRLTVVSPNVPYEPLAAAGITRVESAEEAIRECAAQYPNPHI
                     IVMPDAPYTVGMTA"
     gene            complement(1535..2746)
                     /locus_tag="VFA54_02080"
     CDS             complement(1535..2746)
                     /locus_tag="VFA54_02080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZS49619.1"
                     /translation="MRRRPDQLKPLGTAGAQKAPSGVSVPMCNLRLLLDAAPEWQLYI
                     QEVFDSGQFILARQVDAFEREFAQWLGARYAVGVGSGTDALEISLRCAGVSQAEQEVL
                     TSAVTSPYTALAVLAAGATPRFADINPKTLLLDAEDAGQRITANTAAILPVHLYGQVC
                     ALDRFLNLARMPAAALVQDACQAHGACYRGRPLADFSSYVAYSFYPTKNLGCLGDGGA
                     IVTNDIQTAERARCLREGGQRGDQIAWVRGINSRLDEMQACFLRAFLPHGEEWNAERR
                     RIAAVYDALLRDCEIVQPVERGPDSVCHLYVIRTPHRDALREWLAQRGIASGVHYPTP
                     LHLHPAFRSCGLRKGDLPHAERACDEVLSLPLWPGMPESMAEQVVDAIEGFKSTVARK
                     PAVRSSSQQHC"
     gene            complement(2743..3819)
                     /locus_tag="VFA54_02085"
     CDS             complement(2743..3819)
                     /locus_tag="VFA54_02085"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZS49620.1"
                     /translation="MSTWKEATNEARPVPYSGVRVLITGGLGFIGSNLAIRLVELGAR
                     VVIVDSMVPGCGANLYNVTSVADQVQIIREDIAEAAKFRDVLQETRVIFNLAGEISHI
                     NSMQFPVRDAILNATAQLRFLKECAAHAPGIRVVYAGTRQIYGIPKYLPVDEDHPIDP
                     IDFNGVHKRAAMMYHLLYSRENRMDAAVLNLTNVYGPRMAIRLSHQGFLGAYIRRVAT
                     GEPPEIYGDGEQLRDPLYIDDAVRAFLAAGSAPRLPSRVYNVGGPRPLQIREIAEIAR
                     QAGNAPPAVFKPFPREQRAIHIGSYYTNSTRILRELGWAPEVDFADGIRRTLAYYRGS
                     LRQYLPQTPANPDQIDVEADRAMA"
     gene            complement(3816..5345)
                     /locus_tag="VFA54_02090"
     CDS             complement(3816..5345)
                     /locus_tag="VFA54_02090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS49621.1"
                     /translation="MFCALGFAAVLLASRWALAPRYLITFDEINFALAVENFNPALHQ
                     PQPPGDPLFVMLLKLLSHATSDVETLFLSAALLVSFAAVLLIWAFGESLLGGAGGRIA
                     ALLLLFNPAFWLAALTNPVRLCYAAGATGVALALFQALRKHSERWFLVAAALLGVSSG
                     FRPSLAILLAPLALWTGLRIRLGWRAAARAAFLFLLFVATWLPELAAAAGGWIPFGKL
                     LRSYSYGETRATSILFGAPLGPAAHMAWEALVWSCLGALSWIWAIPWLARRNRGIGLD
                     ALTGRFLMFWFIPGLLFYATFHVGDADHTLSIIPAVCLTGAAVLVSLGHEFPKQTGAV
                     AALAVALNVFLFFKPISQTAKASTYKPVRWLDGYMANLLDTVSDLQKQDKVTAIFYSP
                     ITGWRNLSYYFPEVQIIVVKAQDSGLPAGWLFRHKRAQEWSSKDGLIQVPSCGLLVWV
                     DPDAQPNTTAGGPAMRRRYPVSFTRAAPGRVFDFRGFRFVAAPGDCSAAPYSGTRGER
                     P"
     gene            complement(5786..6517)
                     /locus_tag="VFA54_02095"
     CDS             complement(5786..6517)
                     /locus_tag="VFA54_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS49622.1"
                     /translation="MISKIPSLSAFFPAYNDAASLPALIGKAFAVLSENVDEFEVIVV
                     NDGSQDDTAAVLRRLCSRYGPRLRVVEHDKNRGYGGALRSGFSAARYDLVFYTDGDGQ
                     YDVGELPLLLAKMSPDVGLVNGYKLDRHDPWHRICIGWLYNRLARKLFRIRLRDIDCD
                     FRLLRRELLNSLQLTSTTGSICVELVRKIELTPWRVAEVGVHHYPRTHGRSQFFRLPS
                     LLSTVRQLTALYWNLVIRQQKEDAG"
     gene            complement(6514..7266)
                     /locus_tag="VFA54_02100"
     CDS             complement(6514..7266)
                     /locus_tag="VFA54_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS49623.1"
                     /translation="MMNPSEFENIARAERDFWWYRGMRRILFDVLGAAVGQRPVNRVL
                     EAGCGTGYDALILQERCGWQIFPLDLQREGLQYGRSYGLLRLSQGDVAALPFPPATFD
                     LVLSLDVIVHFPRGEEERPVAEFSRVLAPGGLLVLRVAALDSLRSRHSQFTCERQRFT
                     RRRLVALTSKHGIRLLRCTYANTLLLPVALAKFRLWEPLTRQAPQSGVRPVSPWLNRL
                     LMLPLAAESRWIAAGRNFFIGQSLIFVGEKAG"
     gene            7417..8463
                     /locus_tag="VFA54_02105"
     CDS             7417..8463
                     /locus_tag="VFA54_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49624.1"
                     /translation="MRLFSLAGALLFALAGAAESQQVVVRPAPAVSFPAPVDSNSPAL
                     WMGGQFTLYNSTGLGPIRNIGANQFNLWQSQPVVLGPSIHRPYWIEATWVDEDGSIFA
                     WYHYEPPGVCGSLPLTAPEIGALVSTDGGASFMDLGIILQSGYAPDCSSQNGYFAGGH
                     GDFSVVLGRTRKYFYFLFSNYSGPPESQGIAVARLPFERRNNPFGAVQKYCQGDWLEP
                     GIGGNVTAIFPANVIWSRPDADVFWGPSVHWNTYLKSFVMLLNHSCCSPGWPQEGVYI
                     SYSRSLAEPADWTTPVKILDGVGWYPQVLGLAGNGTDKQAGRVARLYVDGASNWELQF
                     EGQATPVVPGGEKY"
     gene            complement(8552..9823)
                     /locus_tag="VFA54_02110"
     CDS             complement(8552..9823)
                     /locus_tag="VFA54_02110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZS49625.1"
                     /translation="MPPVYEATSLEGPRTERALLWLFVSACALWYLKTSVLEPVQGLP
                     QPSDFSHYYKAAHDLLAGTNPFRDADYDYPPLPALMVIPLAHLKYAAARWVWFLISHA
                     CLLYAAFLMWRYTGGDSIAACCVAGVWAFGGPAAENLALGQIGPLLLLLVTVAYVRTG
                     GVRGASVGLGFALKFLPGVLALAFVLRRERRAIVAMGAVAGACLAAPWAILAGWMTGP
                     ALPSKSSYWMGTPALLNWSIPAVMLRALDASQCRCIPASWIYGNTAGGVNLPRGYAIV
                     SVTVSAILLAACACILAKVCRGMLSEAQMPWALAALTTLSLAASPICWTHYQLLQYPG
                     LAFAGSSFARARATRSVAALAMLAALLYGIPVAVLTKYYHRWGGWSAGSPSTLLLWTS
                     IAPFACLGLWFLFLRHLAASAQQPGRTAALC"
     gene            10257..10781
                     /locus_tag="VFA54_02115"
     CDS             10257..10781
                     /locus_tag="VFA54_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HZS49626.1"
                     /translation="MYKKFTLPALAVAGALAFAGSARAQLKIAVINSQRAVLETAEIK
                     KAAADLEAKYKPQQAEIDRRTKELQAIQEKLDSQRLTPQQVAELQADGQRKQRALQRL
                     TEDVQGDVDRERSEILNRGGQRMQEVVKKLAEEKGLDLVVDASNTIYFKPAMDITNEA
                     IAAYDKQYPLAKTP"
     gene            10794..11225
                     /locus_tag="VFA54_02120"
     CDS             10794..11225
                     /locus_tag="VFA54_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49627.1"
                     /translation="MGYLDEYAEKGLAEERRARKIKRALLSLLVCLVITGALYYWFKN
                     YKEEKRVQEFMTALERGDYAAAYSYWGCKVEAPCPNYDYKSFLEDWGPNSPVGKVQSY
                     RMGISHERGSGVVIPVSINGRKPVVLWVEKSNGVLGFAPPF"
     gene            11411..13006
                     /locus_tag="VFA54_02125"
     CDS             11411..13006
                     /locus_tag="VFA54_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HZS49628.1"
                     /translation="MLRVLALLLLASAGWADQASQLYKQARRLEKEGDDLRAFAVLTR
                     VIGMRPEVQKYQVAVERLRVRAAQSLAALGLPQQAQALDPVNRYLPAALEKRQSLGPP
                     PTDAELREAEQAAPPPELRPAPGPRSFHLRGDARVLYEQVFQAYGLNVVFDSDFTAGP
                     PLRFQLDQVEFRDALHALMSVTGTFVVPLHERVALVAKDTQQKRTELEPTMAELLPIP
                     QTVTPEEANEVGRAVQQVLDIRRLTVDPARRQVLVRDTVSRVRLARALYEELARTRGE
                     IMLDVDLVSVSRETMANLGLTPPTTVPVTAGSRGPANLGGGETLFGIQVASAAFEADS
                     LLSEARLLSRFQLRASDGLPASLHVGQRYPVANAIFSPIVITQQIQTLQQNGQLIQPF
                     PSINFEDLGLVFKVTPRVHDSREVSLALEGEFRQLTGQSFNGVPVISDRKFSSSVRVR
                     EGEISVVSGLAVLRATRTRSGYAGLAQIPILGHLFRRDTWDFEQDDLLITITPHLMIP
                     PPGDQIAQRTLYCGTETRIVSPL"
     gene            13089..14141
                     /locus_tag="VFA54_02130"
     CDS             13089..14141
                     /locus_tag="VFA54_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZS49629.1"
                     /translation="MIREIEEQPETLERTLREELPHVRRFRSFLEKRRPPVIVLAARG
                     TSDNAALFGRYLLETTTGILSSLAAPSVLTLYRSQIQWRGALVVGISQSGESTDTNLF
                     LEAARKGGATTIGVTNSASSSITRLVDELFLVRAGKERSVAATKTYTGQLLMMYLLAY
                     ALGAPLEPARMERLPAAAERALRLKPAIAEMAERYRYMDHAVVVGRGLNYANAFEFAL
                     KMMETCYVVAERFSSADFLHGPIALVERAFPVFLFAPPGPTRNATRTILEKLRKLEAE
                     TIVIGEAGDTTNVPYATRRISVPGGVRPAKGLPADLFTPIPYIVPAQLFAATLAVQKG
                     LNPDRPRTLTKITRTI"
     gene            complement(14275..14625)
                     /locus_tag="VFA54_02135"
     CDS             complement(14275..14625)
                     /locus_tag="VFA54_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZS49630.1"
                     /translation="MEPRKKILKQLKMGTTEILILAILTKGDRYGYDISREVKERSEG
                     FFEFKQGFLYPTLRRMEEAKLIAGYWKDSASGGPDRKYYRLTRQGRARLEASLEAWTD
                     FSKRFNKMLAVAKE"
     gene            complement(14794..14934)
                     /locus_tag="VFA54_02140"
     CDS             complement(14794..14934)
                     /locus_tag="VFA54_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49631.1"
                     /translation="MFKLGAVILIVWGYCFFCQELPLKVSVAMMISGLFSMGWGLLSE
                     KK"
     gene            complement(15171..15635)
                     /locus_tag="VFA54_02145"
     CDS             complement(15171..15635)
                     /locus_tag="VFA54_02145"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HZS49632.1"
                     /translation="MKVQGEYSLPLPQPAAWDLLLNTEVLSRAIPGCESLAPAAPDEY
                     EMKMKIAVSSIQGLFAGKVRISEKTPPASYRLEIQGQGKLGHVRGNGVLQLAPDGNGT
                     KVSFAGDVQVGGLLASVGERMLDMTTKMMIKKFFNALVAQAQKLADSRPAGA"
     gene            complement(15632..16492)
                     /locus_tag="VFA54_02150"
     CDS             complement(15632..16492)
                     /locus_tag="VFA54_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZS49633.1"
                     /translation="MISQPFEYYAPESVSEALRLLAAHEDSRVLAGGQSLIPMMKLRL
                     SAPASLIDLGHIAELNYIVETNGSIRIGAMSTHNQIETSETLRQRCPLLAEAAGRIGD
                     IQVRNLGTMGGSLSHADPAADYPAALQALRAQVRLVSAKGERTLPIDEFLVDAFSTAL
                     EPKEILYEVLVPVDPPGTGWAYLKQQQPASGFAVAGVAAQLGVKGGSISFARVGITGV
                     ASRPYAATAVEAALQGKPATPAVLGEAARLAAQGVEPLADVYAAPEYRAHLAQVWTRR
                     ALDTALSRVK"
     gene            complement(16489..18840)
                     /locus_tag="VFA54_02155"
     CDS             complement(16489..18840)
                     /locus_tag="VFA54_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZS49634.1"
                     /translation="MATTTPTRTETYVGRRLRRREDPRLITGTATYVDDVKLPGMLYA
                     AIVRSPHAAARVKSVRTEAAAKAPGVHAVFTGADVEGVGPVPCAAALPGLRVPKHTIL
                     ATNRVYYVGHPVAVVVASDRYLAADAADLIEVDYDPLPAVADPEKALEPGAPAVHPEW
                     PDNIAFTQHTEGGDCKKAFAEAEKNGVIVRQRIVSQRLVPMAMETRGVVAEWRSGDKY
                     LTIYSSTQIPHLMRTQVALMLGIPENHLRVIAPEVGGGFGSKLNVYAEEALMGFIAMR
                     VNKPIKWIESRRENFQTTIHGRGHVDYAELAATKDGRITGMKLKLIQDLGAYHQLLGP
                     LIPTLSVLMMPGLYDFHNIEADVIGVFTNAAPTDAYRGAGRPEATHVIERMVDILAAT
                     LKMDPAEIRLKNFPPKDAFPFTTATGLNYDSGDYAAPLNRALEILDYSKTREEQRAAR
                     RQGRHYGIGISTYGEICAFGPSPATPAGGWESATVKIEPTAKVTIMTGASPHGQGQET
                     SFAQLAADKLGVTPDDVVVIHGDTSVVQYGIGTFGSRATAVGGAAVYQALDDLRAKCI
                     RFAAPMLGVQPDDVRFEGGVFSSPKSSKTATIGEVALEAHIVRHPQPGLEPGLVATRF
                     FEPSNFAFPFGAHIAVVEIDKETGEIKILRYIAVDDCGNVINPLLVDGQVHGGVAQGL
                     GQALLEEAIYDENGQLITGELMNYAIPKAHHVPEIELDRTVTPSPVNPMGVKGVGEAG
                     TIGCSPALVNAVVDALSPLGVLHVDMPMTPQRIWRLISERSQA"
     gene            complement(18897..19373)
                     /locus_tag="VFA54_02160"
     CDS             complement(18897..19373)
                     /locus_tag="VFA54_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZS49635.1"
                     /translation="MQKRIRVTVNGNTVEANVEPRLLLVHFLREIAGLTGTHVGCETS
                     VCGACTVLLEGRAVKSCTVFAVQADGRKVETVEGLATNGNLHPVQEAFWNEHGLQCGY
                     CTPGMIMTSCQLLSDHPNPSREEIRHGLEGNLCRCTGYMHIVNAVQAAAKKLSARA"
     gene            complement(19674..19892)
                     /gene="infA"
                     /locus_tag="VFA54_02165"
     CDS             complement(19674..19892)
                     /gene="infA"
                     /locus_tag="VFA54_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZS49636.1"
                     /translation="MSKEEGIEVTGTVVEKFPGGMFSVELEDKHVVLAHIAGKLRKNR
                     IRILAGDKVTIELSPYDLTRGRITYRYR"
     gene            complement(20081..22693)
                     /locus_tag="VFA54_02170"
     CDS             complement(20081..22693)
                     /locus_tag="VFA54_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS49637.1"
                     /translation="MPARLPVLDSAWKFCAALQIIAASCAFFLAAPPSAVAQSGGSID
                     GSILDPTGAAIAGASVTIENRITGYQRSAATDAAGAFRFRNVPPNSYHVTASAPGFNP
                     SEQEISVRSAVPLTLKLSLSVAGGQTTVTVEAEGSALENVPYAHNDIDQSSFSKLPAL
                     SPGSALSDAIILGSPGVVSDSNGFFHPLGDHAQVTFSVDNQPISDQQSKQFSTQLPLN
                     AIQSMELITGAPGAEYGDKTSLVVNAVTRSGLGQPPHGSFNAQYGSFGTAGEQASLGL
                     GTAKFGNFLVFNAQRSGRFLDSPEFHPMHDIGNNGTFFDRIDYQPGGRDALHLNLFAA
                     RNWFQIPDTYDQPQQDQRQRVITMNIAPGYQHTFSPQALLTVSPFIREDQVSYFPSAD
                     PFADSPATLGQGRRLLNYGGKVDFSYVRGGHNLKIGVQLMQTRLKENFRLGITDPGFN
                     PVCVDATGTPQGPPSVTDPAACPGFGLEANPDLNLGLVPFDLTRGGSLFRFAGAANIN
                     QFAFYVQDSITWGNWTLNPGLRVDEYDGLSQASGVQPRLGVSYRLKPTSTVLRAAYSR
                     TFETPYNENLVLSSATGAGGLATNVFGAFASKPLQPGRRNQYNVGLQQSFGRFLLVDS
                     DYFWKYTANAFDFDTLFNTPIAFPISWRKSKIDGVSVRFSTPNLHGLQAYTNIGHTRA
                     RYFGPENGGLIFNSPLNTGVFRIDHDQAFQQTTNLRYQRPRNGWWFGFTWRYDSGEVA
                     GSVPDLHAALSLTAAQQAAIGFFCGSQRASLGSPITSCGSPQYGAALLRIPSPGAADD
                     DHNPPRVAPRHLFDLGIGTDNLFRSDGPRRITVQWMVVNLTNKAALYNFLSTFSGTHF
                     VPPRTYNAEIGFVF"
     gene            complement(22793..23080)
                     /locus_tag="VFA54_02175"
     CDS             complement(22793..23080)
                     /locus_tag="VFA54_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49638.1"
                     /translation="MAAYRRPSWKSWLALLATLAFLVVSNAVFLPAHSHGSFEDRTCA
                     LCRSGHMPTLEPYVSTQVQAAQLVEWRIESSELSPILEAAARPNPPRAPPA"
     gene            complement(23259..24320)
                     /locus_tag="VFA54_02180"
     CDS             complement(23259..24320)
                     /locus_tag="VFA54_02180"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZS49639.1"
                     /translation="MAWILLFLLAMNIRPLSESAPNRQPQLAAANGQVGMVFGSGGSI
                     LFAQSKDNGETFSAPVRVATLPVLALGHHRGPRIAFAGKTILISAVSGQTPATDEHAH
                     GLAADGDLLLWRSTDGGRTWSQPVAINDVPRSAREGLHAMAADAQGHAAAVWLDLRAK
                     GTRLYGAFSDDAGLTWSKNLLVYESPAGTICECCHPSLVSAGGGEFVVMFRNVLDGMR
                     DMYVVRVRNGKVVAKPQKTGVNFWPLKACPMDGGSVALENGRVVSAWRREERVFLAEP
                     GREIPLGRGKDVSMTVGKKGPYVAWNDGSRIEVSEPGSAAPVTLSQSGAFPALTALSD
                     GSVLAAWEESGSIATRRLR"
     gene            25363..>25884
                     /locus_tag="VFA54_02185"
     rRNA            25363..>25884
                     /locus_tag="VFA54_02185"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         5077 a         7987 c         7955 g         4865 t
ORIGIN      
        1 ttgcagagga tggtggcggc ggtcgaaaac ctcaccgatc agatttcccg cattttgcct
       61 ccagtgccgc tgcggctcga acccaagagg gaaaccacgg cggccgcggc ggcgggttga
      121 agcgccgttc atgcacgttg atgctccgat gacctaattc ccgggctgcg gcggccagtc
      181 ctgtcccaca gcacaaacgc aaaacgccgc ggcccgcgat tccggcgccc cggttcctgg
      241 gctggcttgt tatgccgtca ttcctactgt gtaaggcgcg tcgggcatga cgataatgtg
      301 gggattcgga tactgcgcgg cgcactcgcg aatcgcctct tcggccgact caactcgggt
      361 aattccggcc gcggcgagcg gctcataggg cacattcggc gaaaccactg tgagccgccg
      421 cattttcttc aggtgactgg cccaggctgt cgccagatct ttgtgccggc cgctgccgcc
      481 gaactcggat cccagacctt cgcgaaattc tcccagcagg atcacgtgcc cgtcctcttt
      541 caccagcggt ttgcgcagcc gcaccaccga agtcagcgcg ttaattccct ggtaaagaga
      601 aatatcgctc gggtacccgc cggcgcaggc aagcactaca tcggcgggcc gggataccgg
      661 tacagttgcc cgttttgcgg tctccgctac agccgcccga tgagcttcca tagggtcgcc
      721 cgcaaaagct cgcacgatct gcttccgccg gttgatcatt acattcaaga gaaaagtcgg
      781 cgccgggacc atcggcccca tggcgtccac ctcggtccgg aacgggttgt cgatttctcc
      841 caagcggcag ggctcgcccg gcagctgctg caaatcgtgc aggccaacga tcgtgtccat
      901 gccggagacg gcgggaaaat atgctttgta tcctccggaa tagccggcaa cattgtgggg
      961 cttgatgaag ccggttaaaa cggccagatc ggactctaca atcgcgcgat tgatcgacgc
     1021 cgggattccc cggggcgagc ggccaatgtc ggccaaatgc cgcgcgtcgc gcgcctggtg
     1081 attcatcacc gtataccgcg caaaaatatc gctgcccaca atttctttca tttcggccgg
     1141 cgtcatcgtg cggtgcgagc cgacccccac cacgatgcga atgccttcgg ctgcaacgcc
     1201 cgccgcgtga agctcgctga tcaccgcagg gagcatcagt ctggatggcg ttggccgcgt
     1261 aatgtcgtca acaacgatgg ccacccgctt cgctccccgc gcctgatccg ccagcgggcg
     1321 cgaaccgatc gggttgcgca agcactcggc aagcaaggca gaaggatccg gcacgccggg
     1381 accgtaatca ggctgcaccc actctacgct caggtcttcc gggaaggaaa cggataaagt
     1441 tgcccggcca tagggaagtt gttttctcat cgacacaatt ttcccacagg cgccggcccg
     1501 gacagcgctc tggcaaaggc tgccagagct cgtttcaaca atgttgctgc gaagaggagc
     1561 gaactgccgg ttttcttgcc acggtgcttt taaagccttc gattgcgtcc acgacctgct
     1621 ccgccattga ctcgggcatg ccaggccaca gcggcagaga gagcacctcg tcgcacgcac
     1681 gctcggcatg cggcaagtct cccttccgca gaccgcagct tcggaatgca ggatgcaaat
     1741 gcaaaggcgt cggataatgg acgccggaag cgatgccgcg ctgcgccagc cattcgcgaa
     1801 gtgcgtcgcg gtgcggcgtc cggatgacat aaaggtggca cacggaatcc ggaccgcgtt
     1861 ccaccggctg gacaatctcg cagtcacgca gcagcgcatc gtagacagcg gctattctcc
     1921 ggcgctcggc gttccactcc tccccatgag gcaagaaggc ccgcagaaag caagcctgca
     1981 tttcgtcgag acgggagttg attccgcgaa cccaggcaat ctggtctccg cgctggccgc
     2041 cttctctaag acagcgcgcg cgctcggcgg tctggatgtc attagtgaca atggcgccac
     2101 cgtcccccag gcagcccaag tttttggttg gataaaaact gtaagccacg taggacgaaa
     2161 aatctgccag cggccggccg cgatagcacg cgccgtgcgc ctggcacgcg tcctgcacca
     2221 gcgccgccgc cggcatgcga gccagattca gaaaacgatc cagcgcgcaa acctggccgt
     2281 aaagatgaac cggaaggatg gccgcagtgt tcgccgtgat tcgttgtcct gcatcctcgg
     2341 cgtcgagcag cagagttttc gggtttatat cggcgaaccg cggcgttgcg ccggcggcca
     2401 ggactgccag cgccgtatat ggcgaggtca ccgcggaggt gagcacctct tgctccgctt
     2461 gcgaaacccc cgcgcagcgc aggctgatct ccagcgcatc agttcccgac ccgacgccga
     2521 ccgcatacct cgcccccagc cattgggcga actccctttc aaacgcatca acctgccggg
     2581 ccaagatgaa ctggcccgag tcgaaaactt cctgaatata aagttgccac tccggcgccg
     2641 catccagcag aagccggaga ttgcacatgg ggacgctgac gccggacggt gctttctgcg
     2701 ctccggcagt tcctagcggc ttaagctgat ccgggcggcg cctcatgcca tggcccgatc
     2761 tgcctcgacg tcgatctggt ccggatttgc cggcgtctga ggaagatact gccggagcga
     2821 accgcggtag tacgcgagtg tgcggcggat gccgtcggcg aaatcaactt ccggcgccca
     2881 ccccagttcg cgcaggatcc gggtggaatt cgtgtaatag ctgccgatat ggatcgccct
     2941 ctgctcgcgg ggaaaaggct tgaaaaccgc cggcggcgca ttgcccgctt gccgcgcgat
     3001 ttccgcgatc tcccggatct ggaggggccg cgggccgccg acgttgtaca cccgcgaggg
     3061 cagccggggc gcgctgccgg ccgccaggaa cgcgcgtaca gcgtcgtcaa tgtagagcgg
     3121 gtcgcgcaac tgttccccat ctccatagat ttcgggcggc tccccagtgg ctacccgccg
     3181 gatgtacgcg cccaggaatc cctgatggga gaggcgtatg gccatgcgcg gcccatagac
     3241 gttcgtcaga ttgagaacag cggcatccat acggttttca cgagagtaca gcaagtggta
     3301 catcatggcg gcccgcttgt gtacgccgtt gaagtcaatt gggtcaatcg ggtggtcttc
     3361 gtccacggga aggtacttgg ggatgccata gatctggcgc gtgcccgcgt aaacgactcg
     3421 gatgccgggc gcatgagcgg cgcattcttt gagaaaccgc agttgggcag tagcgttaag
     3481 aatggcgtcg cgcaccggga attgcatgct gttgatgtgg ctgatctctc ccgccagatt
     3541 gaagatcacc cgggtttcct ggagaacgtc gcggaacttt gcggcctcag cgatgtcctc
     3601 gcggatgatt tgaacttgat ccgccacgct cgtcacgttg taaagattgg ctccgcagcc
     3661 cgggaccata gagtccacga tcaccacccg cgcgcccagt tccaccaggc gaatcgccag
     3721 attgctgcca ataaaaccca gaccgccggt aatgagcaca cgcacgccac tatagggaac
     3781 ggggcgggct tcgttggtcg cttccttcca cgtactcacg gcctttctcc tcgcgtaccc
     3841 gagtagggcg cggccgagca atccccgggg gccgcgacga aacggaagcc gcgaaagtcg
     3901 aaaacacgac ccggggccgc tcgtgtaaat gacacgggat agcgccggcg cattgccggt
     3961 ccaccagcgg tggtgttggg ctgggcgtcc ggatcaaccc aaaccagcaa cccgcaggag
     4021 gggacttgga tgagcccgtc tttgctgctc cactcctggg cccgcttgtg gcggaacagc
     4081 cacccggccg gaagccccga gtcttgagct ttcacgacaa tgatctgaac ctcgggaaag
     4141 taatacgata gatttctcca gcctgtaatc gggctgtaaa aaatcgctgt caccttgtcc
     4201 tgcttctgta aatcgctcac tgtatcgagc aggttcgcca tatagccgtc cagccagcgc
     4261 acgggcttat aggtgctggc ctttgcggtt tggctgatcg gtttgaagaa cagaaataca
     4321 ttcagcgcga cagcgagcgc cgccacggcg ccggtctgct tgggaaactc atgcccaagc
     4381 gagaccagca cggctgcgcc ggtcagacag acggccggga taatggataa cgtatggtcg
     4441 gcgtcgccca catggaatgt ggcgtagaac agaaggccgg gaataaacca gaacatcaaa
     4501 aaacggccgg tgagcgcgtc aaggccgatg ccgcgattcc ggcgcgccag ccagggaatc
     4561 gcccagatcc acgatagagc gcccaggcaa gaccaaacga gggcttccca cgccatgtgc
     4621 gccgccgggc cgaggggcgc gccgaacagg atcgaggtgg cccgcgtctc gccgtacgaa
     4681 tacgagcgca gcagcttgcc gaatggaatc catccacccg ccgccgccgc caattcgggc
     4741 agccaggtgg ctacaaacag caggaacaga aaagctgccc gggccgcagc ccgccatccg
     4801 agccggatac gcagcccagt ccacaacgcc agaggcgcga ggaggatcgc aagactcggc
     4861 cgaaaccccg aggagacccc cagcagagcg gcggcgacca gaaaccaccg ctccgaatgc
     4921 ttccgcaggg cctggaacaa tgccaacgcc acgcccgtag cgccggcagc gtaacagagg
     4981 cgcacgggat tcgttaaggc agccagccag aacgcaggat taaaaagcag cagcagggcg
     5041 gcgatcctcc cgccggcgcc tcccagcagc gattctccga atgcccatat cagcagcacc
     5101 gctgcaaagc tgaccagcag agcggcgctc agaaacagcg tttcgacgtc agaagttgca
     5161 tgggagagca gcttgagcag cattacgaac aggggatcgc ccggcggctg gggctggtga
     5221 agcgcgggat tgaagttttc caccgcgagc gcaaagttga tttcatcaaa cgtgatgagg
     5281 taacgcggag cgagagccca ccgggaggcc agcagcacag ccgcgaaccc gagggcgcaa
     5341 aacactcggc atccggcgcg cgagcgcatg gctgtcattc cgccgatgct gtgtttatga
     5401 tcccggcaag ttagttcact cacccgatcc ccatccaact tccccgccat ccgccggagc
     5461 cccgtttacc ccgggcggcg ctatgtgacg tccaccttat aatacagaaa cctcgctgcc
     5521 cggcggtact cgaaaaacgc gctttgtccg gattcggcgg cgggtctggc tccgaaggcg
     5581 aacccacggg cggtccatca tttttcgtcc tgaagaggga taagcgagtt ctttcgggca
     5641 gggcgccacg gaaaagcgcg tgcaggcttc gatgggccgc gatcgaacga tcgaacaggg
     5701 gccgcggcgt ccggcggtcc ctggtcatcc gaatccttgt ttcgaagaac gccgctcgca
     5761 aaaagcggcc tgagtaagcc ggtctctagc cggcatcctc cttctgctgg cgaatcacga
     5821 ggttccagta cagcgcggtc aactgccgca cggtgctcag cagggaggga agtcgaaaaa
     5881 actgggatcg cccgtgtgtg cgcggataat gatgcacgcc cacctcggcg acgcgccacg
     5941 gagtcaactc gattttgcgc accagctcga cgcagatgct gcccgttgtg gatgtgagct
     6001 gaaggctgtt gagtagctcc cggcgcagca gccgaaaatc gcaatcgatg tctcgcaggc
     6061 gaatccgaaa cagcttgcgc gccagccggt tgtaaagcca tccgatgcag atgcggtgcc
     6121 atggatcatg gcgatccagc ttatagccgt ttaccagccc gacatcgggt gacatcttcg
     6181 ccagcagcag cggcagctcg ccgacgtcgt attgtccatc cccgtccgtg tagaacacga
     6241 gatcataacg cgccgcggaa aatccactgc gcagcgcgcc gccatatccg cgatttttgt
     6301 cgtgctcaac cacgcgcaag cgcgggccgt agcgggaaca aagccggcgc aagacagcgg
     6361 ccgtgtcgtc ctggctgccg tcgttgacca cgatcacttc aaactcgtcc acattctctg
     6421 aaagcactgc gaaggccttt ccgatcaggg cgggtagtga cgcggcgtcg ttataggccg
     6481 gaaagaaagc gctgagcgat ggtattttgg aaatcacccg gctttttccc caacgaaaat
     6541 cagggattgc ccgataaaaa aattccgccc agcggcgatc caccggcttt cggccgccaa
     6601 cggcaacatc agcaagcgat tcagccacgg gcttaccgga cgcacgccgc tctgtggagc
     6661 ttgtcgggtg agcggctccc agagccggaa tttcgccagc gcgaccggga gcagcaaggt
     6721 attggcatag gtgcagcgca gcagccggat tccatgtttc gacgtcagcg caaccagccg
     6781 ccggcgcgtg aagcgctgcc tttcgcaggt gaactgcgaa tgcctgctgc ggagcgaatc
     6841 gagcgcggca acgcggagca ccagcaggcc tcccggcgcg agcactcgtg aaaactccgc
     6901 tacaggccgt tcttcctcgc cccggggaaa gtggacgatt acatccaggc tgagcacgag
     6961 atcgaatgtc gccggaggga atggaagcgc cgccacgtcg ccttggctca accggagcaa
     7021 accgtaggag cggccgtatt gcagcccctc ccgttgcagg tccagcggaa agatttgcca
     7081 gccgcagcgc tcttgcagga tcaaggcgtc atagccggtg ccgcatcccg cttcgaggac
     7141 ccgattgacc ggccgctggc ccaccgccgc gccgagcacg tcgaaaagaa tgcgccgcat
     7201 tccccgatac caccaaaaat ctctctcggc tcgcgcaata ttttcaaact cggacgggtt
     7261 catcatgcgg cggccgcact atcgccggtg gcgttgctcc tgtatgttta tatcgagccg
     7321 caacctaccg ctgcaaccat cgtcctggca atgcctgaca gatgagcctt ccggctgcgg
     7381 gtaacttccg ccgccgggcg agcgtcctgc tatgcaatga gactattttc gcttgccggc
     7441 gccctgctct tcgctctggc cggcgcggcg gagtcacaac aggttgttgt tcgtcccgct
     7501 ccggcagttt ctttcccggc gcccgtcgat tccaacagtc cggcgctttg gatgggcggc
     7561 cagttcaccc tctataactc caccggtctg gggcccattc ggaatatcgg cgccaaccaa
     7621 tttaatctct ggcaatcgca gcctgttgta ctggggccct ccattcatag gccgtattgg
     7681 atcgaggcca cgtgggtgga cgaagacggc tccattttcg cctggtatca ttatgagcct
     7741 ccgggagtgt gcggcagctt gccgctgacc gccccggaaa tcggcgcgct tgtctcgacc
     7801 gacggtggcg cgagcttcat ggatctggga attattctcc agagcggcta tgctcccgac
     7861 tgctcttcgc agaatggata ctttgccggc ggccacggag atttctccgt ggtgcttggc
     7921 agaacgcgaa aatattttta ctttttattc agtaactaca gcggtccgcc ggaatctcaa
     7981 ggcatcgccg tagctcgcct gcccttcgag cgcaggaata atcctttcgg tgcggtgcag
     8041 aagtattgcc agggggattg gttggagcca ggcatcggcg gcaacgtcac ggctattttc
     8101 ccagccaacg tcatctggag ccggccggat gcagatgttt tttggggacc ttccgttcat
     8161 tggaatactt acctgaagag ctttgtaatg cttctgaatc attcgtgctg ctcgccggga
     8221 tggccgcagg agggagtcta tatcagctac agccgcagct tggcggaacc tgccgattgg
     8281 actacgccgg taaaaatcct ggacggtgtc ggctggtatc cgcaggtgct cggcttagcc
     8341 gggaatggaa ccgacaagca ggccggcaga gtggcgcgcc tctacgtaga cggcgcctca
     8401 aactgggaac tccagtttga gggacaggcc acccctgttg ttccgggcgg cgagaaatac
     8461 taaacaaaac cgtggctccc ggacgggcgc gctataatgt gcgctttttt gcggcggagc
     8521 gtgagcggct cagcggcgcc gcacccggtc gtcaacacag cgcggcggtg cgccccggtt
     8581 gttgagcgct tgcagccagg tgacgcagga aaaggaacca gagccccaga cacgcaaagg
     8641 gcgctatgga cgtccataga agcaaagtag aaggcgatcc tgcgctccag ccgccccagc
     8701 ggtgataata ctttgtgagc accgcgacgg gtatcccgta tagcagggcc gcgagcatgg
     8761 ccagcgctgc aactgaccgt gtcgcacgag cccgtgcaaa actcgagcca gcgaaagcta
     8821 aaccggggta ttgcaggagc tggtagtgtg tccagcagat cggagaagct gcgaggctga
     8881 gcgttgtcaa agccgctaaa gcccacggca tctgcgcctc cgaaagcatt ccgcgacaga
     8941 cttttgccag gatgcaggcg caggccgcga gcagaatcgc actcacagta accgagacta
     9001 ttgcataacc acgaggcaag tttaccccgc cggcagtgtt tccatagatc caggacgctg
     9061 ggatgcatct acattgggac gcatccaaag ctcgcagcat aactgcgggg attgaccagt
     9121 ttagcaaggc gggtgtgccc atccaataag aggatttcga tgggagggca ggtcccgtca
     9181 tccaccccgc caggattgcc cagggagcag ccaggcatgc gcctgccaca gcgcccatcg
     9241 cgacgatggc ccgccgctcg cgacgaagca cgaacgccag agctagcact cccggaagaa
     9301 atttcaatgc gaatcccagc ccaacggacg ccccgcggac gccgcctgtt ctcacgtacg
     9361 caacggtcac gagcagcagc aatagcggcc cgatttgccc cagggctagg ttttcggcag
     9421 ccggaccgcc aaaggcccag actccagcca cacagcatgc ggcaatgcta tcgccgccgg
     9481 tgtaacgcca catgagaaac gccgcataca gcagacaagc gtgcgatatc aggaaccata
     9541 cccaccttgc ggcggcgtat ttcaggtgag cgagcggtat gaccatgagc gcagggagcg
     9601 gcggatagtc gtaatcggca tcgcgaaacg gattcgtccc ggctaacaga tcatgcgccg
     9661 ctttgtaata gtgcgaaaag tcgctcggct gcgggaggcc ttgtaccggt tccagaacac
     9721 tcgttttcag ataccacaga gcacacgcgc ttacgaacag ccacaacaga gcccgctctg
     9781 tgcgggggcc ctccagcgat gttgcttcat agactggcgg caccgtattt cacgcacctt
     9841 gcgcggtatt ataccaagcg atgccacgcg acccggttcc agagacttca actcgcggtg
     9901 ttagggtcca ggacggctcg ttcttcgccg ctgcgtacgg tttccctgcc aggcgagctc
     9961 tggccgctac aaaccatgtg ccatttaacc tgccatcacg gaaggccgca ctgcacttat
    10021 aatgcttccg gttctgcgcg ggaatggcat ttttgcggcc gaaagcagca aaggctcggg
    10081 ccgcggcctc atcggcggtt gggcagtgga gaagccggtg gggctgttta aaaaaggcgc
    10141 cgggagttgc aaaatccggc cggcgccggc cagctgatgt ccgctctgct tgtggtccgt
    10201 gtaaaatgaa agacttgccc cggaattctt caggttatcg aggagaagct attgtgatgt
    10261 acaagaagtt tacgttgccc gcccttgccg tagcaggggc tttggcattt gccggctcgg
    10321 cgcgcgcgca acttaagatt gcggtgatca acagccagcg cgccgtgttg gaaactgcgg
    10381 agataaaaaa agccgccgcc gatctcgagg ccaaatacaa gccgcagcaa gccgagattg
    10441 acaggcgaac caaggaattg caggcaatcc aagagaagct ggattcccag cgcctgaccc
    10501 cgcagcaggt cgcagaattg caggcggatg gccagcgcaa gcagcgcgcc ctacagcggc
    10561 tcacagaaga cgtgcaaggc gatgtggacc gcgaacgcag cgagatactc aaccggggcg
    10621 gccaacgcat gcaggaggtc gtaaaaaagc tggccgagga gaagggcctg gatttggtgg
    10681 tagacgcctc caacacgatt tacttcaagc cggccatgga tatcacgaat gaagccatcg
    10741 ccgcttatga caagcagtat ccgctggcca agactcccta gaatcgggcg gatatgggct
    10801 acctggatga gtatgcggaa aagggcctgg cggaggaacg gcgcgcccgg aagatcaagc
    10861 gggccctgct ctcgttgctg gtttgcctgg tgataaccgg cgcgctgtac tattggttca
    10921 agaactacaa ggaagagaag cgggtacagg agttcatgac tgcgcttgag cgcggtgatt
    10981 acgccgctgc ttattcctac tggggctgca aggtggaggc gccctgtccc aactacgact
    11041 acaagagttt tctcgaggat tgggggccga acagcccggt tgggaaggtc cagtcatatc
    11101 ggatgggcat ctcacacgag cgaggctcgg gagtagtcat accggtctca atcaacgggc
    11161 gcaagccggt cgtgctgtgg gtggaaaaaa gcaacggcgt gctcggcttc gcccctcctt
    11221 tttagtgatc cggcaaatag aaggcaaatc tcaagccggt tgccgccgga gccgcaaaac
    11281 ctctacgcgg tgggtgttgc gtggcctgag gcgttttctc gcgcgctcaa accccgttct
    11341 cgacgatttt cccggttttt aaaaaactga gttgcacgtt gcgctcctgc gaagcgtcga
    11401 atcttagttg atgctccgag tactggcgtt gctcctgttg gcctcagccg gctgggccga
    11461 tcaggcatct caactctaca agcaggcccg gcgcctcgaa aaggaagggg acgatctgcg
    11521 tgcattcgcc gtgctgacgc gagtcatcgg catgcggccg gaggtgcaaa agtaccaagt
    11581 cgcagtcgag cggctgcgtg tacgcgcggc gcagagcttg gcagccttgg gactcccgca
    11641 gcaggcgcag gctctggatc ccgtgaaccg ctatctcccc gccgctctgg aaaagcgcca
    11701 gagcctgggg ccgccgccaa ccgatgccga gctccgtgag gccgagcagg cggccccgcc
    11761 gcccgagctc cggcccgctc ccggaccacg cagtttccac ttgcgcgggg atgcccgtgt
    11821 gctctatgaa caggtgttcc aggcttacgg cctgaacgtg gtattcgact ccgacttcac
    11881 cgccggtccg cctttgcggt ttcaattgga tcaggtggag tttcgcgacg ctctgcatgc
    11941 cctcatgtcg gtcaccggga ccttcgtagt tccgctgcac gagagggtcg ctctggtggc
    12001 caaggacacc cagcagaagc gcacggaact cgagccgacg atggccgaac tgctgccgat
    12061 tccgcaaact gttactccgg aagaagcaaa tgaagtgggc cgggctgtac agcaggtatt
    12121 ggacatccgg cggctcacgg tggatccggc ccgccgccag gttctggtgc gcgatacggt
    12181 ctcgcgcgtg cggctggcaa gggcgcttta cgaagagctg gcccgtacca gaggcgaaat
    12241 tatgctggat gtggacctgg tctccgtaag ccgcgaaacc atggccaacc tcggcttgac
    12301 gccgcccact acggttccgg tgactgccgg ctcgcgcggt ccggcgaacc tgggcggagg
    12361 cgaaacgctg ttcggcatcc aggtggcgag cgcggcgttt gaggccgaca gcctgctatc
    12421 cgaggcccgg ctcctgtcgc gcttccagtt gcgggcctcg gacgggctgc cagccagcct
    12481 gcacgtcggc cagcggtatc cggtcgccaa cgcgatcttt tcgccgatcg tgatcacgca
    12541 gcagatccag acattgcagc aaaacgggca gttgatccag cccttccctt cgatcaactt
    12601 cgaggatttg ggtttggtgt ttaaagtgac gccccgcgtc cacgacagcc gcgaggtctc
    12661 tctggcgctc gaaggggaat tccgccagct cacgggccag agctttaacg gagtgcccgt
    12721 aatttccgac cgcaaattca gctcgtcggt gcgggttcgg gagggggaaa tcagcgtcgt
    12781 ttccggactg gcggtcttgc gcgctacgcg cacgcggagc ggctatgccg gtctggccca
    12841 gattccgatt ttggggcatt tgtttcgccg cgacacttgg gatttcgagc aggatgatct
    12901 gctgattacg atcacgcctc atctgatgat tccgcctccg ggcgatcaaa tcgcccagcg
    12961 cacgctgtat tgcggaactg aaacgcgaat tgtttcgccc ttgtagcgct ccggttggta
    13021 gctggccggg taagccgcgc ggtacactac ccttcaccgc acttcctgtc cttactccta
    13081 tgtccctgat gattcgcgaa attgaagagc agccggagac cctggaacgg acgctccgcg
    13141 aggagctgcc gcatgtgcgg cggtttcgca gctttctgga gaaacgccgc ccgccggtga
    13201 tcgtgctcgc cgcccgcggc acgtcggaca acgcggcgtt gttcggccgc tacctcctag
    13261 aaaccactac tggcatcttg tcgtctttgg cggccccttc cgtcctcacc ctctatcgat
    13321 cccaaatcca gtggcgcggg gcgctggtcg tcggaatctc gcagtcgggc gaatcgacgg
    13381 ataccaactt gtttctcgaa gccgcccgca agggcggcgc tacgacgatc ggggtgacca
    13441 attcggcttc cagctccatc acacgcctgg tggacgagct gtttctggtt cgcgccggca
    13501 aagagcgcag cgtggccgcc accaagacct ataccggtca gttgctgatg atgtacctgc
    13561 tcgcctatgc cttgggcgct ccgctggagc ccgcccggat ggagcggctg ccggcggctg
    13621 ccgagcgtgc cctgcggttg aagcctgcta tcgcggaaat ggccgaacgt taccgctata
    13681 tggaccacgc cgtggtggtg ggtcgcgggc tcaattacgc caacgcgttt gagttcgccc
    13741 tgaagatgat ggaaacgtgt tatgtggtcg cagagcggtt ttcgagcgcc gactttctgc
    13801 acgggccgat cgccctggtc gagcgcgcct ttcccgtatt tctcttcgcc ccgcccgggc
    13861 cgacccggaa cgccacccgc acgatactgg agaagctgcg gaagctcgag gccgaaacga
    13921 tcgtgatcgg cgaagctgga gacaccacaa acgtccctta cgcgacaagg cgcatctccg
    13981 tgccgggcgg ggtccgccct gccaaagggc ttccggcaga cctgttcacc ccgatcccct
    14041 acatcgttcc cgcccagcta tttgccgcaa cactggccgt tcaaaaaggc ctgaaccccg
    14101 accggccgcg gactctcacc aagattactc gaacgatcta gatgccccgt ttaacagcct
    14161 tgattccgcg ggctggcgcg gctcgcaacg acacccggcg ccgcagccta gtcatacagc
    14221 gcagccatcc cgatccgcca gcccccccat ttctacaaca gctttgctac agcgttactc
    14281 tttggcgaca gcaagcattt tattgaaccg cttgctgaaa tcggtccagg cctcgaggct
    14341 ggcctccagc cgcgcccggc cttggcgggt gagacgataa tacttcctgt ccggaccacc
    14401 gctggcggaa tctttccaat atccggcgat gagtttcgct tcctccatcc ttcgcagagt
    14461 gggatacaaa aacccctgct tgaattcgaa aaacccttcg ctccgttcct ttacttcccg
    14521 ggatatgtcg tagccatatc tgtcaccttt cgtcagaatg gccaggataa ggatctcagt
    14581 ggtccccatc ttgagttgct tcaagatttt ctttcttggt tccatttccc ctcgtccatg
    14641 actttgtcga taagagcttc ttgcgcgaag cccctcggca gccattcttg atgaatgcgc
    14701 tacgcaaaag caagtctttt atgaaattaa tctaattgcg cgtgcggccg ggaaggcaaa
    14761 tcacggcggg ccggatgcag ccggagcgga ggatcacttc ttttcgctga ggagtcccca
    14821 gcccatgctg aaaaggccgc tgatcatcat ggctacactg accttcagcg gcagttcctg
    14881 gcagaagaag caatagcccc agacgatcag aatgacggct ccaagtttga acatccctgg
    14941 ctcctacaag agtgagcttt gtggcaaagg cggtaccaaa aaacagtgtc tatcaaaaca
    15001 ggccgggact gcaagcactt gggaacatat ctttgcctta gagtggatag tactaaagtt
    15061 ggaattttga gcccagtgaa tgtctgagca acgcgaaatc tcggttttcc ggccgaagat
    15121 cctgaatcga tcccggcgca gccaaccggc gcgcggggca gcgtcgggcg tcacgcgccg
    15181 gccgggcggg agtcggcgag cttctgggcc tgggccacca gtgcgttaaa gaatttcttg
    15241 atcatcattt tcgtcgtcat atcgagcatg cgctcgccga cgcttgccag cagcccgccc
    15301 acttgaacgt cgccggcgaa actcaccttg gtcccattgc catccggcgc aagctgcaag
    15361 acgccgttgc cgcggacgtg accaagcttg ccctggccct gaatctccag gcggtagctg
    15421 gccgggggtg ttttctccga gatgcgaacc ttgcctgcaa acagcccctg gatgctcgag
    15481 acggcgatct tcattttcat ttcgtactca tcgggcgccg caggcgccag gctctcgcag
    15541 cccggtatgg cccggctgag aacctcggta ttgagcagca agtcccaggc cgccggctgg
    15601 ggaagcggaa gagagtactc gccctgtacc ttcatttcac ccgcgaaaga gcggtgtcca
    15661 aagcgcgccg agtccagacc tgagccagat gcgcgcggta ctcgggcgcg gcgtaaacat
    15721 ccgcgagcgg ctcgaccccc tgcgccgcca aacgggctgc ctctcccaag acggccggtg
    15781 tggccggttt gccttgcagc gcggcttcaa cagccgttgc cgcatagggc cgcgaagcca
    15841 cacccgtgat gcctacccgt gcaaaggaga tgctgccgcc cttcacgccg agttgggctg
    15901 ccacgccggc gacggcgaaa cctgaggccg gctgttgttg cttcaggtaa gcccatcccg
    15961 taccgggcgg gtccacaggc acgaggactt catataggat ttccttcggc tcgagcgcgg
    16021 tgctgaacgc atccacgaga aattcgtcga tgggcagcgt gcgctcccct ttggcgctta
    16081 ccagccgcac ctgcgcccgc agggcctgga gggctgctgg gtaatcggcc gcgggatcgg
    16141 catgggaaag gcttccgccc atagtgccca ggtttcggac ttggatgtcg ccgatccgcc
    16201 ccgcggcctc cgccagcagt gggcaccttt ggcgaagggt ttcggaggtc tcaatctgat
    16261 tgtgagtgct catcgcgccg atgcggatcg agccgttggt ttcaacgatg taattcagct
    16321 ccgcgatatg gcccagatcg atcaggcttg ccggggccga aagacggagc ttcatcatcg
    16381 gaatgaggct ctggccgccg gccagcacgc ggctgtcctc gtgagctgcg agcaggcgca
    16441 gtgcctcgga aaccgactcg ggggcgtagt actcgaatgg ctgggagatc atgcctggct
    16501 cctttccgaa atcagccgcc agatcctctg gggcgtcatg ggcatgtcta cgtgcaggac
    16561 gccgagcggc gagagggcgt ccacgaccgc attgaccagg gcgggcgagc agccgatcgt
    16621 tcccgcctcg ccgacgcctt tcacgcccat cggattcacg ggcgagggcg ttacggtgcg
    16681 gtccaattcg atttccggaa catggtgcgc tttgggaatt gcatagttca tcagttcgcc
    16741 ggtgatgagc tggccgttct catcgtaaat tgcttcctcg agcagcgcct ggcccaagcc
    16801 ctgagctacg cccccgtgca cctggccgtc cacaagcagc gggttgatca cattgccgca
    16861 atcatccacc gcgatatagc gcagtatttt gatttctccg gtttctttat cgatctcgac
    16921 cacggcgatg tgcgctccga aggggaaggc gaaattcgag ggttcgaaga accgggtcgc
    16981 caccagccca ggctccaggc ctggctgggg atgccggacg atgtgagcct ccagggctac
    17041 ctcgccgatg gtggctgttt tcgaactctt ggggctcgag aaaacgccgc cctcaaaccg
    17101 cacgtcgtcc ggctgcacgc ccagcatcgg ggctgcgaac cggatgcact tggcgcgcag
    17161 gtcatccagg gcctgataaa ctgccgcacc tccgaccgcc gtcgcccggc tgccgaacgt
    17221 gccgatgcca tactgcacga cggaggtatc cccatggatg acgacgacgt catcgggcgt
    17281 tacgccaagc ttatcagccg caagctgggc gaagctggtc tcctggccct ggccgtgggg
    17341 cgaagcgccg gtcatgatgg ttaccttggc cgtcggctcg atcttgaccg tggcgctttc
    17401 ccaaccgccg gccggcgtcg ccggcgatgg gccgaaagcg cagatttcgc cgtaggtcga
    17461 tatgccgatg ccgtagtgcc gcccctggcg ccgcgctgcg cgctgttcct cgcgcgtttt
    17521 ggagtagtcg agaatctcca gcgcgcggtt cagcggtgcg gcgtagtcgc cgctgtcgta
    17581 gttcaagccc gtagctgtgg tgaacggaaa agcgtctttc ggcgggaagt tcttcagccg
    17641 gatctcggcg ggatccattt tcagcgtggc cgccagaatg tcgaccatcc tttcgatgac
    17701 gtgcgtggcc tcgggccttc ccgccccgcg ataggcgtcg gtcggcgccg cgttggtgaa
    17761 cacgccgatc acatccgcct cgatattgtg aaagtcgtac aggccgggca tcatcagcac
    17821 cgacaaggtg ggaatcagcg ggccgagcaa ctggtgatag gcgccaaggt cctggatgag
    17881 cttcagcttc atccccgtaa tccggccgtc cttggtggcc gccagttcgg cgtaatcgac
    17941 gtggccgcgc ccgtgaatgg tggtttggaa gttttcacgg cggctctcta tccatttgat
    18001 gggcttgttc acccgcatgg cgatgaaccc catcagggcc tcttcagcgt aaacattcag
    18061 cttgctgccg aagccgcccc cgacttcggg cgcgatgacg cgcagatggt tctcgggaat
    18121 ccccagcatc agcgctacct gggtccgcat caggtgggga atctgcgtcg aggaatagat
    18181 ggtcagatac ttgtcccccg agcgccattc cgcgacgaca ccccgcgtct ccatggccat
    18241 cggcaccaag cgctggctga cgatgcgctg ccttacaatc acgccgttct tttccgcctc
    18301 ggcaaacgcc ttcttgcagt cgccgccctc ggtatgctgg gtgaaggcga tgttgtcggg
    18361 ccactccggg tggaccgccg gcgcccccgg ctcaagcgcc ttctccgggt ccgccactgc
    18421 gggcagcggg tcgtagtcta cctcgatcaa atcggccgca tccgccgcca ggtagcggtc
    18481 gctcgcgacc actaccgcga ccggatggcc gacataatac acgcggtttg tagccaggat
    18541 agtgtgcttg ggcacacgca ggccaggaag cgctgccgcg caaggaaccg gccccacgcc
    18601 ctccacgtcc gcaccggtaa agacggcgtg tacgcctggg gccttcgctg ccgcttcggt
    18661 ccgtacgctc ttgacgcgtg cggctgcgtg cgggctgcgg acgatcgcgg cgtagagcat
    18721 ccccggcagc ttcacgtcgt ccacataggt ggccgtcccc gtgatcagcc gcgggtcttc
    18781 gcgccgccgg aggcgccgtc ctacatacgt ttcagtcctg gttggtgttg ttgtagccat
    18841 cgtgccacct ctcggatcgg aagtcgatat cgggattttt cttccccgcg cgccgatcag
    18901 gcccgggcgc tgagcttctt ggccgccgcc tgaacggcgt taacgatgtg catatagccg
    18961 gtgcaccggc aaagattgcc ctccagccca tggcggatct cctcccggct cgggttgggg
    19021 tggtccgaaa gaagctggca ggaggtcatg atcatgccgg gggtgcagta gccgcactgt
    19081 aagccgtgct cattccagaa cgcctcctgc accggatgca gattcccatt ggtcgccagg
    19141 ccttccaccg tctccacctt gcgtccatcg gcctggaccg cgaataccgt gcaggacttg
    19201 accgcgcggc cctccagcag caccgtgcag gctccgcaca cgctggtttc acagcccacg
    19261 tgggttcccg taaggcccgc aatctcgcgc aggaaatgca ccagcagcag gcgcggctcg
    19321 acgttcgctt cgaccgtatt tccgttgact gtcacacgga tccgtttctg catggtgagc
    19381 tcctatggat agggatttca tcatgcagca aacatcgtcc aaaatcaagg gcgcggcgcg
    19441 ggtttgtccg gtgtttcgcg ggccgcaaaa agggtacgga attcggctca ggaagaaagg
    19501 ctcggagagg gatccgggcc gtttcctccg cttgctagcg gatcatccga gcctgggcaa
    19561 gatcttgggc ggctttcgta taagaatttg gatcaggagc cgggaaagca aaaaaccagt
    19621 tccgcccggc ccatatccag gccgcgtcag cgagagccga tagccacggg gttttagcgg
    19681 taccggtagg taatgcggcc gcgggtcaaa tcgtacggcg acagctcgat cgtcactttg
    19741 tcgccggcaa gaatgcgaat ccggttcttg cggagcttgc cggcgatgtg cgccagcacg
    19801 acgtgcttgt cctccagctc cacgctgaac atgccgcccg gaaatttttc tactactgtg
    19861 cctgtaacct cgataccctc ttctttgctc atccgcccat atcataccga ggttcgcggg
    19921 ccccaggcaa caaccggctg cgtcccaacg gccgcggccc gacgaattct ccgggctgcg
    19981 aacggcatcg cccggggcgt tccctgttgg acggactgcg ttgtggcaat cgggaacgcg
    20041 ccccaccgca gagcggcgcg cgctcgcctc cggccggacg ctagaacaca aaaccgatct
    20101 cggcgttgta tgtccgcgga gggacgaaat gcgttccgct gaacgtcgag agaaagttat
    20161 aaagcgccgc cttgtttgtc aaattgacga ccatccattg cacggtgatc cgccgtgggc
    20221 cgtcgctccg gaacagattg tccgtaccga ttccgagatc gaacagatgg cgcggggcta
    20281 cgcgcggcgg gttgtgatcg tcgtcggccg cgccggggga gggaatccgc agcagggccg
    20341 ctccgtattg cggcgaccca caggatgtga ttgggctgcc caggctcgcg cgctggctgc
    20401 cgcagaaaaa accgatcgcg gcttgctggg ccgccgtcag gctgagcgcc gcgtgcagat
    20461 caggcacaga cccggccact tcgccgctgt catagcgcca agtgaagccg aaccaccatc
    20521 cgtttcgggg cctctgatat cgcagattgg tcgtttgctg aaaggcctga tcatggtcga
    20581 tgcggaagac tcccgtattg aggggcgaat tgaagatcag gccgccgttt tccggaccga
    20641 agtaccgcgc gcgtgtgtgg ccgatgttag tgtaggcctg caacccatgt agattgggcg
    20701 tgctgaagcg gaccgaaacg ccgtctatct tcgatttgcg ccaggaaata gggaaagcga
    20761 tcggcgtgtt aaacagcgta tcgaaatcaa aggcgttggc ggtgtacttc caaaaataat
    20821 cagaatccac cagcagaaag cgcccgaaac tctgctgaag accaacattg tattgattgc
    20881 gccggcctgg ctggagcggc ttgctggcaa acgccccgaa gacgttggtg gcgaggccgc
    20941 ccgctcctgt ggcgctcgat aaaacgagat tctcgttgta cggggtctcg aacgtgcgtg
    21001 agtatgccgc gcgcagaacg gtgctggtcg gcttgaggcg ataggaaaca cccaggcgcg
    21061 gctgaacgcc cgaggcctga ctgagaccgt cgtattcgtc gacgcgcagg ccggggttta
    21121 acgtccagtt tccccaggtg atcgaatcct gcacatagaa cgcaaactgg ttgatattgg
    21181 cggctccggc aaaccggaac aagctgccgc cacgggtcag atcaaaaggc acgagcccaa
    21241 ggttcagatc cgggttcgcc tccagcccga agccggggca ggcggccggg tccgtcactg
    21301 aaggcgggcc ctgcggagtc ccggttgcat cgacgcagac cgggttaaag cccggatcgg
    21361 tgatgccgag gcggaaattt tccttgaggc gcgtctgcat cagttgcact ccgattttca
    21421 gattgtgccc gccgcggaca taagaaaagt caaccttccc gccgtaatta agcagccggc
    21481 gtccctggcc cagcgtggct ggagagtctg caaagggatc ggcgctggga aagtagctca
    21541 cctggtcctc gcggatgaac gggctcacgg tgaggagcgc ctgcgggctg aaggtgtgct
    21601 ggtagccggg tgcgatattc atggtgatga cccgctggcg ctggtcctgc tgcggctgat
    21661 cgtaggtatc gggtatttga aaccagttgc gcgcggcgaa cagattcagg tgcagggcgt
    21721 cgcggccgcc cggctgataa tcgatccggt cgaaaaacgt tccgttgttg ccgatatcgt
    21781 gcatcgggtg aaactccggc gagtccaaaa accgccccga ccgctgggcg ttgaatacca
    21841 gaaaattccc gaatttggct gttcccagac ccagggatgc ctgctccccg gctgtgccaa
    21901 aagatccgta ctgtgcattg aagctgccgt gcggcggttg ccccaggccg gatcgggtca
    21961 ccgcgttcac caccagactc gttttgtccc cgtactcggc gcccggggcg cccgtgatca
    22021 attccatgga ttgaatggcg ttcaacggca actgcgttga aaactgcttg ctctgctgat
    22081 cgctgatcgg ctgattgtcc acagaaaagg tcacctgcgc gtgatcgccg agaggatgga
    22141 agaaaccgtt cgagtcggag acgacgccgg gactgcccaa gatgatggca tcgctcagcg
    22201 ccgaccccgg cgatagcgcc gggagtttgg aaaaactgct ctggtcaata tcgttgtgag
    22261 cgtatggcac gttttccagg gccgatccct cggcctccac cgtgacggtg gtctgtccgc
    22321 cggccacgct aagggagagc tttagggtta acggcacagc ggaacgcacg gatatttcct
    22381 gttcgcttgg gttgaacccg ggagcagagg ccgtcacatg atacgagttc ggcggaacgt
    22441 tgcggaaccg gaaggcccct gcggcgtccg tcgcagccga ccgctggtaa ccggtaatgc
    22501 ggttttcgat tgtgacgctt gctcctgcaa tggctgctcc cgtgggatcc aggatcgagc
    22561 catcgataga accgcccgat tgcgcaacgg cgctgggcgg cgcggccaaa aagaaagcac
    22621 aacttgcggc gataatttgt agcgccgcgc aaaatttcca cgcactatcc aaaacaggca
    22681 gtcttgccgg catagtcaac tctccctttc gatcgggcgg aatagacaac gaaagctctg
    22741 cgggctctat gccgcaggcc agggcgcagt tgcatcgatt cgggggaagc gattatgcgg
    22801 gcggtgctcg agggggattc gggcgggcgg ccgcctcgag aatcgggctc agttcactgc
    22861 tctcgatgcg ccattccacg agttgggcgg cttggacctg ggtgctaaca tacggctcca
    22921 gcgtgggcat gtggccgctg cggcacagcg cgcacgtgcg gtcttcgaag gagccgtggg
    22981 aatgggcggg caggaaaacc gcgttagaga cgactagaaa cgccagcgtt gcaagcaacg
    23041 cgagccacga tttccacgaa ggtcggcggt acgcggccat ccaaaggctc agatctgtcg
    23101 gccaatggac gccagaaaga cgccgcaggt ttccgaaaaa gattgggttc agaaatcagc
    23161 gaatcgctcc gggaaaaaaa cggcatgctg tgctcgtgcg ccgccggaag gttggcggcg
    23221 cctgccctca gggtgaagcg gagccgctac ccgcaccctc agcgcaatcg gcgtgtggcg
    23281 attgatccgc tttcctccca cgcagccaga accgagccgt ccgaaagcgc cgtgagcgcc
    23341 gggaaggcgc cggactgaga caacgtaacg ggagcagccg atcccggctc gctcacttcg
    23401 atccgggagc cgtcgttcca cgccacataa ggccctttct tgccaacagt catggaaacg
    23461 tctttgccgc ggccgagcgg gatttcccgc ccgggctcgg caagaaacac cctttcttcc
    23521 cggcgccacg cgctgacaac cctgccgttt tccagcgcga ccgatccccc gtccatcggg
    23581 caggctttca gaggccagaa gttgacgcct gttttctgcg gcttggcgac gaccttgccg
    23641 ttgcgtaccc gtacaacgta catgtcccgc attccgtcaa gcacattgcg gaacatcacc
    23701 acgaactccc cgccacccgc cgaaaccagc gaggggtggc agcactcgca gatggtgccg
    23761 gccggggact cataaaccag taggttcttc gaccatgtaa ggccggcgtc gtcggaaaac
    23821 gcgccataga gacgagttcc cttagcccgc agatcgagcc acacggccgc agcatggccc
    23881 tgcgcatcgg cggccatggc gtgcaagccc tcccgcgccg atcgcggcac gtcgttgatc
    23941 gcgacaggct gggaccaggt gcgcccgccg tctgtggacc gccacagtag cagatctccg
    24001 tctgccgcca ggccgtgcgc atgctcgtcc gtggccggcg tctggccgct gacggcgctg
    24061 atcaaaatcg ttttcccggc gaaggcgatg cgcggcccgc gatgatggcc cagcgcgagc
    24121 acgggcagcg tagcgacgcg gaccggcgcc gaaaaggttt ctccgttgtc cttcgattgg
    24181 gcaaacagga tggagccgcc gctgccgaac accatgccga cctggccgtt cgccgcagcc
    24241 agttgcggct gccggttcgg agcgctctcc gagagcgggc ggatgttcat tgccagcagg
    24301 aacaacagga tccaagccat gacgggcctc ctctaagact atacccgaat cttcgactat
    24361 acccgaatct tcctgtccca tggcccttga ctgggcggga tctcgaaccg cacggcggct
    24421 gccgggctgg caggggcggg ccgccgccgg attcccggga atgccaaacc tgcgccggtc
    24481 gagtcgcgct ccgccgggcg ccggaaggga ccttgtagcg ctctaaagta aaaaacatat
    24541 tgtaaatgtt ttgtttttcg gcgtgccgct cctgcctgcc gctgcttcag ccgagcaatt
    24601 gataccgctc cccgccgccg cgccggcgat tcctctggga ctggatttcc gcctccggtc
    24661 ccggaggaaa acccgctcac cgccgaaaga tcgaaatcgg gccgctggct gttttttaat
    24721 cccgtctctc gcccgacggg ctcattgccc gcacgtgatg gccacgatcc cagagcgcct
    24781 tctctgacgg ccgcccgacc gccgtgggtg tgggccgggg ccgcgggaat gctccggcca
    24841 gggcggagtg cggtcttgtg agcgggggta catgccgggg gcgcacctga aattttccct
    24901 tgcatcctgc ggcgaccttt ggtaatctca tgttgtatca gttgaccgct ggcacctctt
    24961 ggtgtctgcg gtttggaagg ttaggtaaca aaatcccggt catgcgccac gcagccggga
    25021 cggaatacgg cctacctgag cggtctttca agtttttcga aattctggtg ctcggttttt
    25081 ccccgcgagc gcgagttctg tgcggggccg ggcgctgatt ggtttcgagg ccagtctggc
    25141 ctggggagtt gtagctgccc gagaagggca gcggcagcgg cgatccggct gggcgcagat
    25201 ctttgacaat ctggttggat gcaaattcgt aattcgtcat gttttgagtt caaccgggcg
    25261 aacgaaactc acgagttagt tgtgcttccg ccgtgcgggg gtctcctcgc aacgtgccgc
    25321 agccgcaagg cggcggccgg ggtggaaggt gcaacgacaa tttttttcga gagtttgatc
    25381 ccggctcaga atcaacgctg gcggcgcgcc taacacatgc aagtcgaacg agaaagcggg
    25441 ggcaaccccg tgagtaaagt ggcgcacggg tgagtaacac gtggataacc taccatggag
    25501 tggggaataa cctggggaaa cccgggctaa taccgcatac gttccctgga acacaagttc
    25561 cggggaggaa aggcgcaagc cgctccaaga ggggtccgcg gccgattagg tagttggtag
    25621 ggtaatggcc taccaagcct gggatcggta gccggcctga gagggcacac ggccacactg
    25681 gcactgaaac acgggccaga ctcctacggg aggcagcagt ggggaatctt gcacaatggg
    25741 ggaaaccctg atgcagcgac gccgcgtgag tgatgaagcc cttcggggtg taaagctctt
    25801 ttggcgggga aaatgatgat ggtacccgcc gaataagctg cggctaacta cgtgccagca
    25861 gccgcggtaa tacgtaggca gcaa
//
LOCUS       DASREK010000035        17964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1340567, whole genome shotgun sequence.
ACCESSION   DASREK010000035 DASREK010000000
VERSION     DASREK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17964
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340567"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFA54_02190"
     CDS             <1..735
                     /locus_tag="VFA54_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZS49640.1"
                     /translation="NICYLGGEVKDPSRTIPRAVILSLLFVAAIYLLMSTSVIAVVPW
                     QEAARSTAIASDMLARIYGGWAGTAATVLVLWTTFAALFALMLGYTRIPYAAAVDGRF
                     FSVFARLHPRGRFPHVSLLVLGAVSMALCVFDLEAIIKALIVIQIAVQVLAQIVAVTF
                     IRRCRPDIHRPFHMWLYPLPSVVAFALWSYVLVTSGWRFIAGGFAVLFAGIVLFLCRA
                     RSHREWPFRPAAEAGQGEKATAETGQ"
     gene            complement(1081..4377)
                     /locus_tag="VFA54_02195"
     CDS             complement(1081..4377)
                     /locus_tag="VFA54_02195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49641.1"
                     /translation="MRFLKIMNLVLVVLLWGLPAWAQIDTGTITGTVTDPTGAVVPKA
                     VVTATNEATNVSTTTQTNSQGQYVLSGLKVGTYSVSAELAGFRKIVRTGLELHVQERL
                     AVDLALQVGEVTQAVEVSAGTPVLETQSADMGNVVEQRRVVDLPLNGRHYEDLALLAP
                     GVFPRPGTGNPSPARFNVNGNFSLHNYFALDGVDNNTAGEGLFGGSPQIIQPPPDAIQ
                     EFKVQTRTYSAEFGTAQGAVINAALKSGTNQLHGDLYHFLRNSALDANDFFANTAGIK
                     KGNFVRNEGGFTVGGPVYLPGVYDGRDKTFFFSDFSFLRIRKAQTLAATVPTPAMKKG
                     DFSGLASLKDPSSVIPSEGGCIVNNVIQQRCIDPVGLAYANLYPDPTVPGPFTGQANY
                     FANASVPTNSNLVDAKIDERASDKDSMFVRYSYYKPVALVEVGPFTAVNPLSTGGFTA
                     NNPVTSQQAATSWTHVFSPTLVNEARFSFSRRNAAETPFSPPGSAADKVGLKNVPNTP
                     LSSGIPAMNVGGFPKLGTSEWRPQFQVSQVWQALDNLSMVKGKHSFKYGFEWKRYTQN
                     TLDIRAPQGAMTVDNNLYVGASGTSSFAGLANLLLGNVTTFNLSSPFVYHDYMNGTNF
                     YAQDTWRLTSTFTLNYGVRYEYFTPWIERDNHTSNFDSANGGRVILASNGGVFQRALV
                     NPDKNNFAPRVGFAYNPTKRVVVRGGFGVFYQAYDVVGSESVVQLNPPQFIDTAIGVG
                     PTQPPVFFLRNGYPTLSPPSITDPKFLTQIQFRAMDQNMRSPYVEQASLGVQFDLGHD
                     TVFELSGVGNYAHKVRKLRNLNQGILTTYGNPASVVFPYQPTFGNGFIEYLAANGDTN
                     FHALEMRVEKKYSNGLSFLASYTWSKSLGDVLDNLSAGFSGGVQVFPQNAYNNRADYG
                     PSAFDQRHRFVLSYVYELPWGKGKHWLNGPGPVTYALGNWQINGITTFSTGAALTATA
                     PASLSGTAPNGGANARANCLSPVTTGGPLNAWFSKSSFGIPAAGTFGTCGPNTFYGPG
                     LNNWDFSAFKKFPVTESKYFEFRSEFFNLWNHPQFAPPNTSVTSGGFGQITSLQVPTR
                     EIQMALKFYF"
     gene            complement(4572..5447)
                     /locus_tag="VFA54_02200"
     CDS             complement(4572..5447)
                     /locus_tag="VFA54_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZS49642.1"
                     /translation="MKHTRRDFLAAFTAAGAMAGPLAAQQPAADQITRAELIAGSRLP
                     DAIRAPARSPAVLPLGSIEYHGPGAALGTDTLLGSGLGERVCRRLGRSNIPAVLFPAV
                     VYSQSPAHTRAFSGTISVRPEAVTMYLHDILEGISAMGFQKILVLNAHDGNIGPGRAA
                     VFQIANEKPLAQFLFLNWWEAVPGPLVDSLHLFTQGNGGHGHGGPLELSSVAAVNPAA
                     LIRERGPDLPAPPDIAGFPYYLAKYSSPAWPGYSGKVSEASAEKGEKIIELAAERIAA
                     LVERWIANAEIPGNW"
     gene            complement(5444..7243)
                     /locus_tag="VFA54_02205"
     CDS             complement(5444..7243)
                     /locus_tag="VFA54_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS49643.1"
                     /translation="MPRRLETSSAKSPDAGSLGNASAVFSKGFVIAIAARGLLSPNAV
                     ILAVLLLLFLPAAAAATGPPSAQAVKRFQDAVAQWKQGHPEAAEPVLKELCSEYPDEP
                     AFQDALGTLYSLQRQPRLATPYLEQAARLKPSADANAKLAENYAELGRSREAEALFQK
                     ALALEPGNIGANMGLASLKMDRDAYAAAAPLLERVVRARPADPQPAYLLAVCYSSLGR
                     AAQARRVLLNLPEKARDREEIQLLLGSASLALHRQDEARAYFERAFQRNPASVPAAAN
                     LGGLLVAAGELERGLKLLEAAWQSDHTSYLAGYNLALAYRTKGDFAAARNVLTSLLAQ
                     GEKAELYSLLGEVEIELRDGPAALDHLQRAVKLEPGEADLFELGYALLQFWKLNEAVE
                     VLSRGTAQFPSSARLWMALGTAYFAQDRNQDAIDAYLRATQLGDDPRAYRFLAEVYQA
                     ANASRSDVAARFHHYRLTHPNDAWANLCDGYGLLRDGQPEKALPLLERAVQLAPGLAA
                     AHFELGKLYSQQGRREEALRAYEAAVKADPGHPEAWYRLGQAYMRAGRREDAEKALAR
                     HEELRRQQAAGIEQRLRGAIELLNDSKRKQDQP"
     gene            7775..8686
                     /locus_tag="VFA54_02210"
     CDS             7775..8686
                     /locus_tag="VFA54_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZS49644.1"
                     /translation="MALLDLICIGEFYSDFIFYGLDRVPELGEEVKTEHFLRSPGGGA
                     PITALTARRLGLRVGLVTVVGSQTARADLAPLKEAGVETAYSLIHPSRPTGITVSVST
                     ITDRFFLTCTGANRDLERLLPADLFSYCARARHVHLALLPRNFARWTALCRRLAACKI
                     TTSWDHGWNPDIAAKRGARALLRAVTIFLANRTEGEHWTGQRRPEAALEALARYAALP
                     VLKLGACGAAAWERNPSDKGHPGGRMILAPAERIRGAANGVETTGAGDAFNAGFLAAF
                     IGGARLKECLLRGNQAAAQRVAAQKRK"
     gene            8683..10017
                     /locus_tag="VFA54_02215"
     CDS             8683..10017
                     /locus_tag="VFA54_02215"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HZS49645.1"
                     /translation="MKLTVIGGAGARVPLLLHGLIERRRELPLEEVVLYDLARDALDL
                     MGGVCRDLLRRDGEAFRLRIVASLEDALEGASFVLTSIRAGGMAARAADERFLLDRGL
                     LGQETVGAAGFAKALRTIPPMLEIVRATLRGAPRAWIINFTNPVGIVTQAIRTALPEA
                     RIIGICDTPLELFHGLAHAAGAPFERLRFDYFGLNHLGWVKKVMDGGRDVLPQILADP
                     GRIRTVYERPIFSPELLAGLGAFPSDYLYFYYSPEAAVAHLRAAGQTRAEQILEMNRW
                     LADELSRGGGLAAYEKYLAGRNATYLQLETDMPRPPQQLYSASAGYDRIALAVLTALH
                     GDTGDIIALDVDNEGAIRGYDRTATVEVPCAVGRRGPRPLDAGAAPQPVAALLRRVKD
                     YEDLTVQAALEASLEKASKALAANPLIDSRSLAEDLVAEFVRMHPKLLGYLR"
     gene            complement(10208..10768)
                     /locus_tag="VFA54_02220"
     CDS             complement(10208..10768)
                     /locus_tag="VFA54_02220"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZS49646.1"
                     /translation="MKQTSGWLLIAALFGTAAWGAEPAERSAMSRSFDRQLSGVEREV
                     VSLAEALPADKFAFAPTQGEFQGVRTFAQQASHIAATLYMIASALLGEKNPSEAGKDE
                     NGPASLKTKQDVVNYLKAAFAYAHKAMNTLTDQNATEMIGAPFGNNKVPRVSLAVMAI
                     SHTFDHYGQMVVYARMNGVVPPASRR"
     gene            complement(10847..12313)
                     /locus_tag="VFA54_02225"
     CDS             complement(10847..12313)
                     /locus_tag="VFA54_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HZS49647.1"
                     /translation="MRRPWKSVGRFLPLVLAAAALLPAQQTRPAWRPARAGSCPALII
                     GNPDGNYIVPGSMGDIRYQGGLTMDAYAPPGNPRPAALVIRGSRGNKRGYVTSVYEQL
                     ARDGYAWFAPSFRDAEDVAAALRFIRCPGRFNITGKIVLIGDDTGAQIALQIASRGGI
                     AGVVALAAKLDETAARPGVPVLMIHGAQDEEWPLAKARAFCSELKRCTLYVEHDARHA
                     FESWPAAQWDYKEELDAWLIGDRRGLWNDIAFSRPDGRALTMNAYIPAGPGPFPAVII
                     AHGGGWEGGDKVVYVAPIFRPLAKAGVAWFSIDYRLLPYVRNPEQVEDLRAAIRYIRQ
                     HAARYHVDTSRLAILGESASGQLVTLVGAEPCPGCEVQAIVSFYGVYKLEEGGRFASR
                     LDWMFGPDRTLDTLRRYSPLFAAHAGMPPVLVLQGDKDPLKAGSDEYVEQLKKLGVPH
                     EYVIVEGAPHGMENWEGHPEWAFYKQKVVDWLKATMHF"
     gene            complement(12590..13471)
                     /locus_tag="VFA54_02230"
     CDS             complement(12590..13471)
                     /locus_tag="VFA54_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49648.1"
                     /translation="MLRWRSAVLLFLSFPGIFLLLNSCSSGPPQPKMGTPAWYWEAAN
                     EQFTAGDLAKTQEHLERVVASDNPYKARAAVWHLVMSSGMAMGYKELAEAYDAGGEEV
                     KDKASQAAEFRRLGNDARRSAKQYCLALAQELESFQKENAGATEFALEFAFPQGSPGE
                     DPALARVRKGMLPPESERAQLERATVKRGVVLETASLVGADGDPAKAADMFKTQPVKV
                     PRAVFLYGLAESLFEESKIFDRKQLNEPDKKKILYQLALDCLKPASEAGDEGLKKKAK
                     SLEDKIAKEQKTLPKNV"
     gene            complement(13782..14363)
                     /locus_tag="VFA54_02235"
     CDS             complement(13782..14363)
                     /locus_tag="VFA54_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZS49649.1"
                     /translation="MADEVYRGAEREGPLTPSFWGRYEKVLITGGLSKAYGVPGIRLG
                     WIAGPAEEVASLWGCHDYTTIAVGVLSDALARTVLQPENRRRILERTRGVLRENYLCL
                     AQWAEEQNGILRFVPPAAGTVAFFRYHLHIHSTDLMRKLRDEKSVLVVPGEHFGMDGF
                     LRIGYGQPLSILQAALGRISEVFHEIQSREARR"
     gene            complement(14282..14914)
                     /locus_tag="VFA54_02240"
     CDS             complement(14282..14914)
                     /locus_tag="VFA54_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZS49650.1"
                     /translation="MRVEPFEMERLQSLWENRVEYNLSESGVHPMSLREVLGDDISLE
                     EFLDQKLGYGQTNGSPQLQESIAELYPSADAENVLVTNGAAEANFLAVCSRIEPGDEI
                     IVMLPNYMQIWGLARALGAQIVPLPLREQHRWAPDLGQLARAFSRRTRLIAVCNPNNP
                     TGAVPPNMKCRPSRTPHGKPGRGSWRMRCIGAPSERAPSRPASGAGTRRF"
     gene            15077..15625
                     /locus_tag="VFA54_02245"
     CDS             15077..15625
                     /locus_tag="VFA54_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS49651.1"
                     /translation="MKDASKMLVRAALEDPEAFAAIVERYKAMVFSVAYHFFQNRGLA
                     EDLAQEVFLELYRRLDRLESDVHLAFWLRRAISNKCIDYARRSKVNSDVALDAVPELP
                     AEAVSRDPLLAGRLRRAVQALPKRKRLVVILRFQEELELAEIAEVLQMPLNTVKSTLH
                     RTLSLLKKDLRKEGVPSLEAIH"
     gene            15627..15935
                     /locus_tag="VFA54_02250"
     CDS             15627..15935
                     /locus_tag="VFA54_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49652.1"
                     /translation="MDRLEEELSRCLRRESPSLDFTARVMKGVRARRRPVALWRRWAL
                     SSALAASLVTGVYIRHEAAERARAESAKSQLILSLQIASSKINKAREAVIRSAREDPL
                     "
     gene            15932..16486
                     /locus_tag="VFA54_02255"
     CDS             15932..16486
                     /locus_tag="VFA54_02255"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HZS49653.1"
                     /translation="MKGAALIACLLACAPAFGQGAKLQLNLDHLAAKAAEVANVNLEG
                     PMLEMGRKFLSRKDADKDVKALVDSLTGVYVRSFEFSKPGDYTQADVESIRKQLNAPG
                     WTKFIDVQESKNGETTCVYSYLENGKMAGLAVLAAEPRELTVVNIVGPIDLSKLGQLQ
                     GQLGIPDLKLEMRPRSSKPATAKK"
     gene            complement(16706..17524)
                     /locus_tag="VFA54_02260"
     CDS             complement(16706..17524)
                     /locus_tag="VFA54_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase"
                     /protein_id="HZS49654.1"
                     /translation="MFHRRSFLVAGALAAARAFPLPKQAASGVLRLNLRTRVEAFKGS
                     GLWQEVHFEQTLSAAQTAVIICDMWDNHWCTGAARRVQALALKMAPIVDEARQHGVQI
                     IHAPSETMDFYAQSPQRQRIASLPKIAPPANLNLPDPPLPIDDSGGGCDTPDKFYKAW
                     TRENATLHIGANDVISDNGAEIYSFLQQRGIKNLLVMGVHANMCILNRPFAIKQMTKW
                     GLRCILVRDLTDAMYSPNDRPYVSHEQGAELVIEHIEKYWCPSVLSADLRRALH"
     gene            complement(17536..>17964)
                     /locus_tag="VFA54_02265"
     CDS             complement(17536..>17964)
                     /locus_tag="VFA54_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS49655.1"
                     /translation="RALKRDPEARELLYGRAKSLIGLGEAEQAAADLEALVRAEPAYH
                     FYDAYLTLAGCYERLGRDDAARAAYQEILGRTTVSRAYYDYGRLLARLGELETAREMM
                     RQILAKKLSLPRYLRRQERPWFRKAEAFLKSGAGAGAKTM"
BASE COUNT         3314 a         5557 c         5532 g         3561 t
ORIGIN      
        1 aacatctgct atctcggagg ggaagtgaaa gatccgtcgc gaacaattcc ccgggcggtg
       61 attctctcgc tgttgtttgt ggctgccatt tatctgctga tgagcaccag cgtgatcgcg
      121 gtcgtgccct ggcaggaagc agcgcgctcc actgccattg cttcggacat gctcgcccgc
      181 atctatggcg gctgggcagg aacggccgcc acggtcctgg tgttgtggac gacatttgcg
      241 gctctattcg cgctgatgct cggctacaca cgcattccct acgccgctgc tgtggatggg
      301 cgcttcttct cggtgttcgc ccggctgcat ccccgggggc gatttcctca cgtatccctg
      361 ctggtgctgg gggcggtctc gatggcgctg tgcgtcttcg atcttgaggc gattatcaag
      421 gcgctcatcg taattcagat cgcggtgcag gtgctggcgc aaattgtcgc cgtgacgttc
      481 atccggcgct gccggcccga catccatcgg ccattccata tgtggctcta cccgctgccg
      541 agcgtagtgg cgttcgcatt gtggagctat gtgctggtca cttccggctg gcgctttatc
      601 gccgggggct tcgccgtgtt gtttgccggc atcgtcctgt tcctctgccg ggcccggtcc
      661 caccgggagt ggcccttccg cccggcagcg gaagccgggc aaggagagaa agcgacagcc
      721 gaaaccggcc agtaaaggcc tctctgccga tcgattcaac cgccttgcgc cccggctctt
      781 tggggcaatc ccgccgccgg ccgttttttt gcggggacag ccttcctgag caacagcctt
      841 cctgaacaac ggataaaggg aaggagagac ccggcgccgg caacaacctt ttccgcgcca
      901 gttctgagaa cagtcaatca gccttggacg attctcgcgc tattgtcgcc cggttgacca
      961 aaccaaaggc cggcttccgt ttttgccgcc gaaagccggc tcgggttttg cggccccctc
     1021 aacccgctga gttggcgagt tgccattact gcgccggagg cgcgatgccg cgtccagcgg
     1081 tcaaaagtag aacttgagcg ccatctggat ctcccgtgtg ggcacctgca acgatgtgat
     1141 ctgcccgaat ccgcccgagg tcacgctcgt gttcggcggc gcgaactggg gatgattcca
     1201 caggttgaag aactcgctgc gaaactcgaa gtacttggat tccgtcacag ggaatttctt
     1261 gaacgccgaa aagtcccagt tattcagccc tgggccgtaa aaagtgtttg ggccacaggt
     1321 gccgaacgtg ccggcagccg gtattccgaa cgagctcttg ctgaaccagg cgttgagcgg
     1381 tcctcccgta gtcacggggc taagacaatt ggcgcgcgcg ttggcgccgc cgttcggagc
     1441 agttcctgag aggctcgcgg gagcggtcgc cgtcagtgcc gccccggtgc tgaatgtagt
     1501 gattccattg atctgccagt tgcccaacgc gtaggtcacc gggccgggac cgttcagcca
     1561 gtgcttcccc ttgccccagg gcagctcata gacgtagctc agcacgaagc ggtggcgctg
     1621 gtcgaaagcc gatggcccgt aatcggcgcg gttgttgtac gcgttctggg ggaagacctg
     1681 cacgccgcca ctgaagcctg cggagagatt gtccagcaca tccccgagac ttttgctcca
     1741 ggtataagaa gcgaggaagc tcaatccgtt ggaatacttc ttctccaccc gcatttccag
     1801 cgcgtggaaa ttggtatctc cgttggcggc cagatattcg ataaagccgt tgccgaaagt
     1861 gggctgataa gggaatacca cgcttgcagg gtttccgtac gtggtcagga tgccttgatt
     1921 caagttgcgc aacttacgaa ccttgtgcgc atagttccca actccggaaa gctcgaacac
     1981 cgtgtcgtgg ccgagatcga actgcacgcc caggctggct tgctcgacat acggcgaacg
     2041 catgttctgg tccatggcgc ggaattgaat ctgtgtgagg aacttcgggt cggtgatgct
     2101 gggaggcgac agagtcgggt agccgttgcg gaggaaaaag accgggggct gggtcggccc
     2161 cacgccgatg gctgtatcga tgaactgcgg cgggttgagt tgcaccacgc tctcgctgcc
     2221 caccacgtca tacgcctgat aaaagacgcc aaatccgccg cggactacca cccgcttggt
     2281 cgggttgtag gcgaagccca cgcgcggcgc aaaattattt ttgtcgggat tgacgagcgc
     2341 ccgctggaat acgccgccat tgctcgctaa tatgacacgg ccaccattgg ccgagtcgaa
     2401 attggaagtg tgattatcgc gctcaatcca cggcgtgaag tattcgtagc ggacgccgta
     2461 attcagcgta aaggtcgacg tgaggcgcca ggtatcctgg gcatagaagt ttgtcccgtt
     2521 catgtaatcg tggtagacga acgggctgct gaggttgaag gtggtcacgt tgcccaggag
     2581 gaggttggca agtccggcga agctgctggt gccgctcgcc ccgacataca aattgttatc
     2641 caccgtcatg gcgccctgcg gcgcgcggat gtcgagcgta ttctgggtgt agcgcttcca
     2701 ctcgaagccg tatttgaagc tgtgtttgcc cttcaccatc gagaggttgt ccagcgcctg
     2761 ccacacctga gaaacctgga attgcggccg ccactctgaa gtgccgagtt tcggaaagcc
     2821 gcccacgttc atggcgggaa tgccggagct cagcggggtg ttcggtacat tcttcaaccc
     2881 gactttgtct gcggcgctgc cgggcgggga aaacggcgtt tcagcggcgt tccgccgcga
     2941 aaaggagaat cgggcttcgt tcaccagcgt ggggctgaac acatgggtcc aggaagtggc
     3001 cgcctgctgg ctggtcaccg ggttgttcgc ggtgaaaccg cccgtggaga gcggattgac
     3061 tgcggtaaaa ggccccacct caaccagcgc caccggcttg taatagctgt agcgaacgaa
     3121 catgctgtcc ttatccgagg ccctttcgtc tatcttggcg tccaccaggt tcgaattggt
     3181 cggaacgctg gcgttggcga agtaattcgc ctgccccgtg aacggccctg gaaccgtggg
     3241 gtccgggtac aggttggcgt atgctaggcc aaccggatcg atgcagcgct gctggatcac
     3301 gttgtttacg atgcagccgc cctcggaagg gatcacgctg ctcggatcct tcagtgacgc
     3361 cagcccgcta aaatcgcctt tcttcatcgc aggggttgga accgtagcag ccagcgtttg
     3421 ggcctttcgg attcgcagga aactgaaatc ggagaagaag aacgtcttat cgcggccgtc
     3481 ataaacgccc ggcagatata cggggcctcc cacagtgaat ccaccctcgt tccggacgaa
     3541 atttcccttc ttgattcccg ccgtattcgc aaaaaagtcg ttggcatcga gcgctgagtt
     3601 ccggaggaag tggtacaggt cgccgtgcaa ctggttagtc ccggatttca gcgcagcgtt
     3661 gatcaccgct ccctgcgccg tgccgaattc agcggaataa gtgcgggtct gcaccttgaa
     3721 ttcctgaata gcatcgggcg gaggctggat gatttgaggc gagccgccga acaacccctc
     3781 gccggccgtg ttgttgtcca cgccgtccag ggcgaaataa ttgtgcaggg agaaattgcc
     3841 attcacgtta aaccgcgcgg gggaaggatt gcccgtgccc gggcgcggaa atacgccggg
     3901 cgccagcaaa gccaggtctt cgtaatggcg gccgttcagc gggagatcca caacgcggcg
     3961 ctgctcgaca acattgccca tgtcggctga ctgcgtctcg agtaccggag tgccggcgct
     4021 cacctccacc gcctgggtga cttcgcccac ctgcaaggcg agatccaccg ccaggcgctc
     4081 ctgaacatgc agctccaagc cggtgcgcac aattttgcgg aagcccgcca gttcggcgct
     4141 gacgctgtag gtgccgacct tcagcccgct gagtacgtat tgcccttgcg aattggtctg
     4201 cgtggtcgtg ctgacgttgg tcgcctcgtt ggtcgccgta accacggctt tgggcacgac
     4261 cgcgccggtc ggatcagtga ccgtccccgt gattgtaccg gtatcgatct gcgcccatgc
     4321 gggcaaaccc cacagtaata ctactagtac cagattcatg attttcaaga aacgcataga
     4381 tattcctcct ggcgcacaca gacaccgact actgaaaacg ggcagaccaa acaaccgtga
     4441 aagcgccacg gtacgtctcc ctttgttacg caatacgcaa aacagaccct actgttttct
     4501 tgagtatacc gcggtgcacc gatttgtcaa gattgcggcg aaccctgcga aagctcaaat
     4561 cgccaataaa atcaccagtt tccggggatc tctgcgttcg cgatccagcg ctcgaccaag
     4621 gcggcgatcc gctcggcggc cagctcgatg atcttctcgc ctttctccgc cgaggcctcg
     4681 ctgacctttc cggaatagcc tggccaggca ggagaagagt atttggccag atagtaggga
     4741 aatccggcga tatccggcgg cgcgggcagg tccgggcctc tctctcgaat caaagcggcg
     4801 gggttgaccg ccgccaccga ggaaagttcc agcggtccgc cgtggccgtg tccaccattg
     4861 ccctgagtga acagatgaag ggaatccacc agaggaccgg gaacggcttc ccaccagttg
     4921 agaaaaagaa actgagccag cggtttctcg tttgcgatct ggaaaacggc ggcccggccg
     4981 ggtccaatgt tgccatcgtg ggcgttcagc accaaaattt tttgaaagcc catcgcgctg
     5041 atgccctcca gaatatcgtg cagatacata gtaacggctt ccggacgcac cgaaatggtc
     5101 ccgctgaagg cccgcgtgtg cgcgggcgac tggctgtaaa ctaccgccgg aaacaagacc
     5161 gccggaatat tggaacgtcc gagtcgccgg cacacgcgct cccccagccc gctcccgagc
     5221 agcgtatccg tgcccagggc cgcgccgggc ccgtggtatt cgatgctgcc cagcggcagc
     5281 acggcgggcg accgcgccgg agcccggatg gcatccggga ggcgggagcc tgcgatcagc
     5341 tccgcccggg tgatttggtc cgcagcgggt tgctgcgccg ccaaggggcc tgccatcgca
     5401 cccgcggccg tgaatgcggc caaaaagtcc cgtcgtgtgt gtttcatggc tggtcctgtt
     5461 ttctctttga atcgttgagc aattcaatgg cgccgcgcag ccgctgctcg atgccggcag
     5521 cctgctgcct gcgtagttcc tcatggcggg ccaatgcctt ttcggcgtcc tcccggcggc
     5581 cggcccgcat gtaggcctgt cccagacggt accacgcttc cgggtggccg gggtcggcct
     5641 taactgctgc ttcataggcc cgcaacgcct cttcccgccg gccttgctgg gaatacagtt
     5701 tgcccagctc gaaatgggcc gccgcgaggc ctggcgcaag ctgcacagcg cgctcgagca
     5761 agggcaaggc cttctcgggt tggccatcgc gcagcagccc gtagccgtcg cacaaattag
     5821 cccaggcgtc attcggatga gtaagccggt agtgatggaa cctggcggcc acatcggagc
     5881 ggctggcgtt tgctgcctgg taaacttcgg ccaggaagcg gtaagcgcgc ggatcatcgc
     5941 cgagctgcgt cgcgcgcagg taggcgtcga ttgcatcctg gttgcggtct tgggcgaagt
     6001 acgccgtgcc aagcgccatc cacaggcgcg cggagctcgg gaattgggct gtgccgcgac
     6061 tcagaacttc cacggcttcg tttaatttcc agaactggag caatgcatat cccagctcga
     6121 acagatcggc ctcccctggt tccagcttca ccgcgcgttg cagatggtcg agggcggccg
     6181 gcccgtcacg aagctcgatt tccacttccc ccagcaggct gtaaagctca gctttttcgc
     6241 cttgagccag cagagaagtg agcacgttgc gcgctgcggc gaagtctccc tttgtcctat
     6301 aggccagcgc aaggttgtag ccggccaggt aagaagtgtg gtcgctctgc caggcggctt
     6361 ccaggagctt gagaccgcgt tcgagttccc cggcggcgac cagcaaaccc ccgaggttgg
     6421 ccgctgccgg gaccgatgcg ggattccgct gaaaggctcg ctcgaaatac gccctggctt
     6481 catcctgccg gtgcagcgcg agcgaggctg agccgagcag gagttggatc tcctcgcgat
     6541 cgcgagcttt ttccggcaga ttgagcaaca cgcgccgcgc ctgcgccgcc cggcccagag
     6601 acgagtagca gacggccagc agatacgccg gctgcgggtc ggccggccgc gcccggacca
     6661 cgcgttccag cagcggcgcc gccgccgcgt acgcgtcccg atccattttc agggaggcca
     6721 gacccatgtt tgcgccgatg ttgcccggct cgagcgccag cgctttctgg aacaacgctt
     6781 ccgcctcgcg gctgcggccg agctcagcgt agttttcggc aagcttggcg ttggcgtcgg
     6841 ccgagggttt gagccgcgcc gcctgttcca ggtagggagt cgcaagccgc ggctggcgct
     6901 gcaagctgta gagcgtcccg agagcgtcct gaaacgccgg ttcgtcagga tactcgctgc
     6961 acaactcctt caagaccggc tccgccgctt cgggatgccc ctgcttccac tgggcgacag
     7021 cgtcttgaaa tcgtttaacc gcctgggctg agggaggtcc ggtggcggcg gccgccgccg
     7081 gaaggaaaag cagaaggaga acggcgagga tcacggcatt cggcgaaagc aggcccctcg
     7141 ctgcgatagc gatgacgaaa cccttggaga aaacagcgct ggcgttgccc agggaaccgg
     7201 catccgggga cttcgcgcta ctggtttcga gtcgtcgcgg cacgtccgga tgccgcctcg
     7261 gcggcggctt cttcactgta gcactgtcgc gagcgggtaa agcgagtgcg agcgggcaac
     7321 gttcggaaag cagttagagc ccggggacgt ctggcgctcg ggcaactaac actggattcc
     7381 aaccccagcg cagcgaggga tctgcctggg tccttttcgc cgccgaatcc tccgacgttg
     7441 ggtttgcgcc tcgatcagcc ccactttttt tctcatgccg ctacctttca aaatctgatc
     7501 ttcagaggct tttggagggc gctgagtggc aaagacgcct ccacaccgcg gccttttacc
     7561 tggggcttgc ggtcacgatc cctttcctcg tgttgtaatg ggcttagggc agccctcgcg
     7621 ccacaaagag cagagcggcc gcctgacggc agcaatgacc tttgggagtt gtggcggagt
     7681 gtacaatcct tcggaacagt tcatgggcgg cgtcgatcta tggcttgcca cctgtttctg
     7741 tcgcccggct gctgcctgaa agatcccctg accaatggcg ctgctggatc tgatttgcat
     7801 cggcgagttt tactccgatt tcatctttta cggcctggat cgcgtgccgg agctgggcga
     7861 ggaagtgaaa acggagcatt ttctgcgctc gcccgggggc ggagcgccga tcaccgcctt
     7921 gacggcgcgc cggctggggc tacgcgtggg cctggtcaca gtggtgggat cccagacggc
     7981 acgggcggat ttggcgccgc tcaaggaggc tggcgtggaa actgcatact cattgattca
     8041 ccccagccgg ccgacaggca taaccgtatc ggtctcgacc attacggacc gcttctttct
     8101 gacctgtacc ggagccaatc gcgacttgga aagactgctg ccggctgatc tgttttccta
     8161 ctgcgcccgc gcccggcacg tgcatctggc gctgctgccc agaaacttcg ctcgctggac
     8221 ggcgctatgc cggcgcctgg ccgcctgcaa gattacaacc tcctgggacc atggctggaa
     8281 tccggatatc gcagccaaac gcggcgcccg ggcgctgctg cgggcggtca cgatttttct
     8341 agcgaaccgg actgaagggg agcactggac cggccagcgg cggccagagg ccgcacttga
     8401 agcgcttgcg cgttacgcgg cgctgcccgt gctgaagctg ggcgcatgtg gggcggccgc
     8461 ctgggagcgg aatccctcag acaaggggca tcccggcggc cggatgatcc tggcgcctgc
     8521 ggagcgaatt cgcggcgcgg caaacggcgt cgaaaccaca ggcgctgggg acgccttcaa
     8581 cgccggcttc ctggccgcct ttatcggcgg agcccgattg aaagaatgcc tgcttcgggg
     8641 caatcaggcg gcggcgcagc gcgtggcggc gcaaaaacgc aaatgaaact gacagtcatc
     8701 ggcggggcag gagctcgtgt tcctttgctg ctccacggtc ttattgagag gcggcgcgag
     8761 cttcctttgg aggaagttgt gctctacgac cttgcccgcg acgccctcga tctcatgggc
     8821 ggcgtgtgcc gggatttgct gcggcgcgat ggtgaagctt tccggctgcg catcgtggcg
     8881 agcctcgagg acgcattgga gggcgcctca ttcgtgctga ccagcattcg cgccggcggt
     8941 atggccgccc gcgcggccga tgaacgtttt ctgctcgacc gcgggttgct gggtcaggaa
     9001 accgtcggcg ccgccggctt cgccaaggcg ctgcggacca ttcctccgat gctggagatc
     9061 gtccgcgcga ccctgcgcgg cgctcctcgc gcctggatca tcaatttcac caacccggta
     9121 ggaatcgtta cgcaggcgat ccgcacggcg ctgccggagg cgcgcatcat cggcatctgc
     9181 gacacgcccc tcgagttgtt tcacggcttg gcgcacgccg ccggcgcgcc ctttgagcgg
     9241 ctccgcttcg attatttcgg gctgaatcat ctgggctggg tgaagaaagt gatggatggc
     9301 ggccgcgacg tgctgcccca aattttggct gatcccgggc gcatccgaac cgtatacgag
     9361 cggccgattt tttctccgga gctgctcgct ggcttaggcg cgttcccttc cgattatctc
     9421 tatttctact attctcccga ggcggcggtg gcgcacctgc gcgccgcggg ccaaacgcgc
     9481 gccgaacaga tcctggaaat gaaccggtgg ctggcggacg agctttcgcg cggcggcggc
     9541 cttgcggcgt atgaaaaata cctggccggg cgcaacgcca cctatttgca gcttgaaacc
     9601 gacatgccgc gcccgccgca gcaactttat tcggcctccg ccggctacga ccgcatcgcg
     9661 cttgctgtgc tcaccgcgct ccacggcgat acgggcgaca ttatcgccct cgatgtggac
     9721 aacgagggcg cgattcgcgg ttacgatcgc acggcaacgg ttgaagtgcc gtgcgccgtt
     9781 ggccgccgcg ggccccggcc gctcgacgcg ggagcggcgc ctcaaccggt ggcggctctg
     9841 cttcgccgcg tcaaggatta cgaagacctc accgtccagg ccgcgctcga agcgtcactc
     9901 gaaaaagctt ctaaagcgct agccgccaat ccgctgatcg acagccgctc tcttgccgag
     9961 gatttggtcg ccgaatttgt gcgcatgcat cccaagctcc ttggttatct gcgatagttt
    10021 cgaaagcaca gacgccgctt ctcggaaacg gaagggcagg ggccggcgct tccggcgctg
    10081 ctggggcgcc aagacgcccg cggcgaggcc ggccgaaacg gcgcggctcc attcggcagt
    10141 tggcttcccg gacagatatc ccacgcgacg ccggcctgat tcaactcccg ccgttcgagc
    10201 tgcggcctca gcgacggctg gcgggcggca caacgccgtt catccgggca taaaccacca
    10261 tctgcccgta gtgatcgaag gtatgcgaga tggccataac ggccaaggaa acacgcggca
    10321 ctttgttatt gccgaagggc gcgccgatca tctctgtcgc attctggtcg gtcagcgtat
    10381 tcatggcctt gtgcgcgtag gcaaaagccg ccttcaggta attcacgacg tcttgcttgg
    10441 tcttgaggga cgccgggccg ttttcgtctt tgccggcttc gctcggattc ttttctccca
    10501 gcagagcgga agcgatcata tagagcgtcg cagcaatatg ggaggcctgc tgggcgaagg
    10561 ttctgacgcc ctgaaactct ccctgagtgg gcgcaaacgc gaatttgtcc gcgggcagcg
    10621 cttcggcgag ggacaccact tctcgctcca cgccgctcaa ttgccggtcg aaactgcggc
    10681 tcatggcaga gcgctcggcg ggttccgcgc cccatgccgc cgttccgaat aacgccgcta
    10741 tcaacagcca accgcttgtt tgtttcatcg ctgtcgctcc tatgtttttt gcgtggcgcg
    10801 ccatctcaag ccaaatgttc caaccttgcc gggccggcgg cgcttctcaa aaatgcatcg
    10861 tcgccttgag ccaatctacg actttctgct tgtaaaaagc ccactccgga tgtccctccc
    10921 aattctccat tccgtgagga gcgccttcta caatcacata ttcgtgcggc acgcccaact
    10981 tcttcaactg ctctacatat tcatccgatc cagccttgag cggatccttg tcgccctgca
    11041 ataccagcac cggcggcatt ccggcgtgcg cggcgaaaag gggagagtag cggcgcagcg
    11101 tatccagagt ccggtcggga ccgaacatcc agtcgaggcg tgaggcaaac cgcccgcctt
    11161 cttcaagctt gtagacaccg taaaaagaca cgatcgcctg cacttcgcag cccgggcacg
    11221 gctcggctcc caccagagtg acaagctgcc cactggccga ttctcccaga atcgcgagcc
    11281 gactcgtgtc aacgtgatac ctggcggcat gctggcgaat atagcgaatg gccgcgcgca
    11341 aatcctcgac ctgctccgga ttgcgcacat acggcagtag ccggtaatcg atggaaaacc
    11401 atgccacgcc ggccttggcc agcggcctga atattggggc aacatagacg accttgtcac
    11461 cgccctccca accgccgccg tgggcgatga tgaccgccgg aaagggcccg ggcccggccg
    11521 ggatatacgc attcatggta agagcacggc cgtcagggcg tgagaaagca atgtcgttcc
    11581 acaggccgcg gcggtcgccg atcagccacg catcgagttc ctctttgtaa tcccactggg
    11641 cggcaggcca gctctcgaag gcgtggcgtg cgtcatgctc cacataaagg gtgcagcgct
    11701 tcaactcgct acaaaaggcc cgggctttgg ctaacggcca ttcttcatcc tgggcgccgt
    11761 gaatcatgag caccgggacg ccgggacgtg cggccgtttc atccagtttc gctgccaagg
    11821 cgaccacacc ggcgattccg cctctcgaag caatctgaag ggcgatttgc gcgccggtat
    11881 cgtccccaat caaaacgatt ttgccggtga tgttaaaccg gcccgggcaa cggatgaaac
    11941 gtagggccgc tgcgacgtct tcggcatcgc gaaagctagg ggcgaaccag gcgtaaccgt
    12001 ctcgggcaag ctgttcgtac acactggtca cataaccccg tttattcccc cggctgcccc
    12061 gaatcacaag cgcagcgggg cggggatttc cgggcggcgc gtaggcatcc attgtgaggc
    12121 cgccctgata acggatatcg cccatcgacc ccgggacaat atagtttccg tccgggtttc
    12181 cgatgatcaa agctgggcac gaccccgccc gagcgggtct ccaagccggc cgcgtctgct
    12241 gcgccggcag caaagcggcg gcagccagca cgagcggcag aaaccgccct acgcttttcc
    12301 acggccttct catgaacgcc tctcgggagc cgccagccac gctatcaaag accggaaaag
    12361 actgtcaacc tgaagacgcc gagaagaccg cttgcaaccc ggacgaagct tggcagaacg
    12421 ccgcggcggc ttcagaaggt atgaggtcta aggcggcaat cggtggagcg agttccctcc
    12481 cgccggcaag ttgctggatc cttcagcggg caaacatttc ctccggcggt tttaccgcag
    12541 aggcagcgcg tgcctgcctt ctcccaacct gcttcgccgg cgagggtccc tacacgttct
    12601 tgggcagggt cttctgctct ttggcgattt tgtcttcgag cgatttcgct ttcttcttca
    12661 gaccttcgtc gccggcttcg gaagccggtt tcaaacaatc gagagccagt tggtagagaa
    12721 tcttcttctt gtcgggctca ttcaattgct tgcgatcgaa gattttggat tcttcgaaca
    12781 agctctccgc cagcccataa agaaacacgg cgcgcggtac ctttaccggc tgggtcttga
    12841 acatgtccgc agccttcgcc ggatcgccat ccgctcccac cagagacgcc gtttcgagca
    12901 cgacgcctcg tttcacggtg gcgcgctcga gttgggcccg ttcggactcc ggtgggagca
    12961 tccctttacg cacccgggcg agcgcgggat cctctccggg gctgccctgt ggaaatgcga
    13021 attccagagc aaattccgta gccccggcgt tttccttctg gaagctttcc agttcttggg
    13081 cgagagccag gcagtactgt ttggcagacc gtctggcgtc gtttcccaaa cgccggaact
    13141 cggccgcctg cgacgccttg tccttcactt cctccccgcc ggcatcgtag gcctccgcca
    13201 gttctttgta gcccatcgcc atgccgctgg acatgacaag gtgccagacg gccgcgcgcg
    13261 ccttgtaggg gttgtccgaa gcgacgaccc gttcgagatg ctcctgtgtc ttagccaagt
    13321 cgcccgccgt gaactgctcg ttggccgcct cccaatacca ggccggggtg cccatcttgg
    13381 gctgaggcgg gcccgatgag cacgaattga gcagaagaaa aatccccgga aaggacagaa
    13441 acagaagaac cgctgaacgc cacctcagca tgctctcctc cttatgtttc gctaaacttg
    13501 gccaatgctg taccacaact cagccgagga tgcaagggcc gtcgagctgt caactaacca
    13561 atcggtgaat ttttcatcct gcgatgccgg ccggatccgt tcggcgtgta gcttgagggc
    13621 ttagcagccc gaatccgacg aggggcgtcg ttttaacagc gcagcgagac atatcaggta
    13681 atccgtcgag ggtaacaggt ccccatgcag cacaggtttc tcccggaaat tgcaaagaga
    13741 atccgctttt cccgaacggc tccgcgtttt cactggaaaa ttcaacgccg ggcttctcgg
    13801 ctctggatct catggaagac ttcggagatg cgccccagag cggcttgcag gatgcttaaa
    13861 ggctggccat agccgatccg taaaaagccg tccatcccga aatgctctcc tggaactact
    13921 aggacgcttt tctcgtcccg cagtttgcgc atcaagtctg tcgaatgaat atggaggtgg
    13981 taacgaaaga aagcgacagt tcccgcggcg ggcggaacga agcgcaggat cccgttctgt
    14041 tcttccgccc actgcgcgag gcagagatag ttttcgcgca ggacgccgcg cgtgcgctcg
    14101 agaatccggc gccggttttc cggctgtaga acagttcggg ccagggcgtc gctcagaact
    14161 cccacagcga tggtcgtgta gtcgtggcaa ccccagagag aggcgacttc ttctgccggc
    14221 ccggcgatcc agccgagacg gatgccggga actccatagg ccttcgagag cccgccggtg
    14281 atcaaaacct tctcgtaccg gccccagaag ctgggcgtga gggggccctc tcgctcggcg
    14341 ccccgataca cctcatccgc cacgagccac gccccggttt cccgtgcggc gtccgcgatg
    14401 gcctgcattt catgttcggc ggtacggcgc ccgtaggatt gttgggattg cagacggcaa
    14461 tgagccgggt ccggcgggaa aaggccctcg ccaactggcc caggtcgggc gcccagcgat
    14521 gctgttcccg caggggcagc gggacgatct gcgcgcccaa cgcgcgggcc agtccccaga
    14581 tctgcatgta attcggcagc ataacgatga tttcatcgcc cggttcgatc cgactgcaca
    14641 cggccagaaa attcgcttcg gcggcgccgt tagttaccag cacattttcc gcatccgcgc
    14701 tgggatagag ctcggcgatg gattcctgga gttgcgggct gccgttggtt tgtccatagc
    14761 cgagtttttg gtcgagaaat tcctcgagcg aaatatcgtc gccgagcact tcgcgcaggg
    14821 acatgggatg gacgccgctt tccgacaggt tgtactcaac gcggttctcc cacagcgatt
    14881 gcaggcgctc catctcgaaa ggttcgactc tcatggctgg gccaccagat aggagcttag
    14941 agggcgtgga gaacgtggca gcagtacgca accctccagg aatggccggc gttcgagcct
    15001 ctcgcgccgg caataaaaaa agtcctcgct ggcggaccct cgcgccgccc gcttgcgtat
    15061 ataaccttgg cacgcgatga aagacgcttc caagatgctg gtgcgcgcgg cgcttgagga
    15121 cccggaggcg tttgcggcca ttgtggaacg ttataaggcc atggtcttca gcgttgccta
    15181 ccatttcttc cagaaccgcg gcctggcgga ggacttggcc caggaggttt ttctggagtt
    15241 gtaccgccgc ctggaccgcc tggagtccga cgtacatctg gctttctggc tgcggcgggc
    15301 catcagcaac aagtgtatcg actacgcgcg acggagcaag gtaaacagcg atgttgcttt
    15361 agatgcggtc ccggaactgc ccgccgaggc ggtctcgcgc gacccgttgc tggccgggag
    15421 actgcgccgc gccgtgcaag ctttgccgaa gaggaagcgc ttggttgtca tcctccgctt
    15481 tcaggaagag ctggagctgg ccgagattgc cgaagtcttg cagatgccgc tgaataccgt
    15541 gaagagcacc ctccaccgga cgctctcact gctgaaaaaa gacctccgga aggaaggtgt
    15601 gccgagcctg gaggcaatac actagcatgg accggctgga ggaggaattg agccgctgtc
    15661 tgcggcgcga atcgccgtcg ctggacttta cggcccgggt gatgaagggc gtccgtgcga
    15721 gacgccgtcc agtggcgcta tggcggcgct gggcgctgtc cagtgctctg gcggcctcct
    15781 tagtgaccgg cgtctacatc cggcatgaag cggccgagcg cgctcgcgcc gagtccgcca
    15841 agagccaact gatcctgtcg ctccagatcg ccagctcgaa gatcaacaag gctcgcgaag
    15901 ctgtcatccg ttctgctcgg gaggatcctt tatgaaaggt gctgcgctta ttgcttgctt
    15961 gctggcttgc gcgcccgcgt tcggccaagg cgctaagctg caactcaatc tcgatcatct
    16021 ggccgccaag gccgctgagg tggccaacgt gaacctcgaa ggccccatgc tcgaaatggg
    16081 ccgcaaattt ttatcccgga aagacgcgga caaagatgtc aaggcgctgg tcgatagtct
    16141 gaccggcgtt tacgtgcgta gcttcgagtt ctcaaagccg ggtgactaca cgcaggccga
    16201 cgtcgagtcg attcgaaagc agttaaacgc gccgggctgg acgaagttca ttgatgtgca
    16261 agagagcaag aacggcgaga caacctgcgt ctactcgtac ctggaaaacg gcaagatggc
    16321 cggcctggcc gtgctggccg ccgagccgcg cgagttgacc gtggtgaata ttgtggggcc
    16381 gatcgacttg agcaagctgg gccagcttca ggggcaactc ggcattccgg acttgaagct
    16441 cgagatgcgg ccgcgctcct ccaagcccgc aacagccaaa aagtgattgt ttcagtgcgg
    16501 cagcaacttc cgcgcgggtg gtgggttcgc ggcggcccat tcccgctgcc tcctcgcggc
    16561 aggttggcgg accccctcca tgattgccgc ctgctacgcc ctgctttgcg ccggcttcag
    16621 tgagtttggc tccaatgcct cgccgaactt cgccaagtct tgactggcgt cgcgagggat
    16681 gcgtctccca tgaaaacggc cgattttaat gcaaggcccg cctcaggtcg gccgacagca
    16741 cagatgggca ccagtatttc tcgatgtgtt cgatcaccag ttcggcgcct tgctcatgac
    16801 tcacgtaggg ccggtcattg gggctgtaca tcgcgtcggt gaggtcgcgg accaggatgc
    16861 agcgcaggcc ccacttcgtc atttgcttaa tagcgaaagg gcggttaagg atgcacatat
    16921 tcgcgtgaac gcccatgacc agaagattct ttatgccgcg ctgctggagg aacgagtaga
    16981 tttcggctcc gttgtcggag atcacatcgt tcgcgccgat gtgcaatgtc gcgttctcgc
    17041 gagtccaggc cttgtaaaac ttgtcaggag tgtcgcagcc gcctccggag tcgtcgatcg
    17101 gaagtggagg atcggggagg ttaagattcg ccggcggggc gattttggga aggctggcga
    17161 tccgctgccg ctgcgggctt tgcgcgtaaa agtccatcgt ttccgaggga gcgtggataa
    17221 tctgcacgcc gtgttggcgc gcctcatcga cgatgggggc catcttcagc gccagggcct
    17281 gcacgcggcg cgccgctccc gtacaccagt ggttatccca catgtcgcaa atgatgaccg
    17341 ccgtttgggc ggcagacaaa gtctgttcga aatggacctc ctgccagagg ccactgccct
    17401 taaaggcctc gacccgcgtg cgcaggttca gccgcagcac tccggatgcg gcctgtttgg
    17461 gtaaggggaa cgcgcgggcg gccgccagag cgcccgcgac aagaaaactc ctgcggtgaa
    17521 acatatcccc tccgttcaca ttgtcttggc gcccgcgccc gctccggatt tcagaaatgc
    17581 ttcggctttc cggaaccatg gccgttcctg gcgccgcaag tagcggggca acgacagttt
    17641 tttcgccaga atctggcgca tcatctctcg cgctgtctcc aactcgccca gccgcgccag
    17701 cagtctccca taatcataat aggcccggga aaccgtggtg cggcccaaaa tctcctggta
    17761 ggccgcccgc gccgcatcat cgcggccgag ccgctcatag catccggcga gcgtcaaata
    17821 ggcatcgtag aaatgatacg ccggctcggc gcgcaccagc gcctccaggt cggccgcggc
    17881 ttgttcggcc tccccgagcc cgatcaggct tttcgcccgg ccatacagga gctcgcgagc
    17941 ttccgggtct cgtttcagcg cgcg
//
LOCUS       DASREK010000036         4429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1177026, whole genome shotgun sequence.
ACCESSION   DASREK010000036 DASREK010000000
VERSION     DASREK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177026"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VFA54_02270"
     CDS             complement(<1..410)
                     /locus_tag="VFA54_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49656.1"
                     /translation="MSGTDPSVEVLIVEQRPRSLAALRETLEPEFTVLAARAEDALAA
                     LEQRAPAVMVCPETAGRDLLKAASERSPATQIVLARRRGRRSAPAAGEGLVYSYIEEP
                     WDPKLLRVLTANAAAYSRLYLKFLRERDLLRALM"
     gene            complement(544..2139)
                     /locus_tag="VFA54_02275"
     CDS             complement(544..2139)
                     /locus_tag="VFA54_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS49657.1"
                     /translation="MQEAESAILTGLAETLEKNLSVEALLDELLRRCLEETGLSGGLA
                     YMLDQDARFCLRAKRGSSDLEASVLESVPGRLALLGQAIESREPLLISRFAAAGPIRD
                     WLDLARAETVLFAPLLMGAQRIGALVLVSSARELDEGWRVFARTLGRHIGPIIRLAAA
                     FCSLRQNEEHLSRIVQTLAEGVLIADRDGRFTLANAAAERILGLPAGEITKRTHNDPC
                     FRARSPDGELLQSAQHPCDRAVATGEPVRNVEMWIDQPDGRRVFVSANASPLRDAAGE
                     ITGVVLSLNDITDYKRQQESLARSNAELKQFAYVVAHDLKEPLRTVASFTQLLAERYK
                     GALDEKADQFISRAVSGTQRMARLIDDLLAYARAGSQAPDPQPVAAEAVFQSALANLE
                     AAIQSSGATVAHTPLPVVLADPSELNQLFQNLIGNALKFHNDAPPSVEVSAERKGGEW
                     IFSIRDNGIGIDPKHADRIFQVFERVHSHKQYSGSGIGLATCKKIVERHGGRIWVESE
                     PGKGSTFRFVLPAEAAAGVEPAG"
     gene            complement(2330..3205)
                     /locus_tag="VFA54_02280"
     CDS             complement(2330..3205)
                     /locus_tag="VFA54_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HZS49658.1"
                     /translation="MIKSAIIPVAGLGTRLLPATKSQPKEMLPVAKKPIVQYVVEELA
                     AAGVERMLFITGRGKSSIENHFDHDPELSRSLNKAEKNGLLDELRFEQLKVHYFYTRQ
                     RLQRGLGDAILCGENFAGSEPFLVALGDSIIGLNGSSTITRRIAEVHESERAACVIAV
                     EEVPRELTSLYGVVAPESEGEVFGIRDLIEKPAPGGAPSNLAIAGRYVFSPAIFEMIR
                     KVEPDRRGEIQLTDAIRMLAVAGERIVGVKLSGGERRYDIGNFRSYFETFIEFALADP
                     QHGPPLRRRLRSLLG"
     gene            3254..4273
                     /locus_tag="VFA54_02285"
     CDS             3254..4273
                     /locus_tag="VFA54_02285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZS49659.1"
                     /translation="MRKAPARPHNPNLGSYYNTVIFQTTAADAGQRLDQFLARHFEET
                     SRSRLQQWIRAGHVLVNGAVAKASARLQGGEQIEVRPLAPPVPARAFPQEIPLEILYE
                     DDDLVAVNKPAGMTVHTGAGITQGTLVNALLHRFKRLSAVGGELRPGIVHRLDRWTSG
                     VLLAAKTDAAHVRLGRQFALRQVRKTYLALAHGRVSGSRQGRPVVLDGFEWRRLEMPI
                     RRDRRYRAKMTARAREGRTALTDYRVIEEWPGFSLLEVRIGTGRTHQIRVHLSTIGHP
                     VAGDTLYGAPHQPTLERIFLHARELVFTHPSTGETLKILAPLPPELESQLQALREGRS
                     ALVLL"
BASE COUNT          843 a         1435 c         1339 g          812 t
ORIGIN      
        1 tccatcagcg cccgcagcag atcgcgctcg cgcaaaaact tcagatacag gcggctgtag
       61 gcggccgcgt tggcggtgag cacgcgcaaa agttttgggt cccagggctc ttcgatatat
      121 gaatagacaa gcccctctcc tgccgccggg gcgcttctcc ggccgcgcct tcgagcgagc
      181 acaatctggg tcgccggcga gcgttcggat gctgctttca aaagatccct gccggcggtt
      241 tcaggacaaa ccatcaccgc cggagcacgc tgctcaaggg cggcgagcgc atcttccgcg
      301 cgggcggcaa gcacggtgaa ttccggttcg agggtttctc gcagagctgc taggctccgc
      361 ggcctttgct ctacgatcag tacttcgacg ctcgggtctg ttccactcat gtcggatggc
      421 gagaaaaccg gccgcacgcc ggccagcgga ataccagcgt cctcgcgccg ctggcgcgga
      481 gaatccgtca gcggggtctc cgatcggcgc ccgggtttct gaccggcgcc ggcccggttg
      541 tattcagccc gccggctcga cgcccgcagc agcttcggcg gggagaacga atcgaaaagt
      601 ggaccctttt ccgggctcgg actccaccca gatgcggccg ccatggcgtt ccacgatttt
      661 cttgcaagtg gccaggccga ttccgctgcc cgaatactgc ttgtggctat gcacgcgttc
      721 gaagacttga aaaatccggt ccgcgtgctt cgggtcgatg ccaatcccgt tgtcgcgaat
      781 ggaaaaaatc cactcgccgc ctttccgctc ggccgagact tctacgctgg gcggcgcatc
      841 gttgtggaac ttaagcgcat ttccgatcag attttgaaac agttgattca gctcgctcgg
      901 atcggccaaa accaccggca gcggcgtgtg ggccacggtg gccccgctgc tttgaattgc
      961 cgcctccagg ttggccagag cgctttgaaa aacggcctcc gccgcgaccg gctgcgggtc
     1021 gggggcctgg gaacccgccc gggcgtaggc gagcagatcg tcaatcagcc gggccattct
     1081 ctgcgttcca ctcaccgccc gggagatgaa ctggtccgcc ttttcgtcca gggctccctt
     1141 ataacgctcg gcgagaagct gcgtgaagct ggcgacggta cgcagcggct ctttcaaatc
     1201 gtgagccaca acgtaagcga attgcttcaa ctcggcgttg gagcgcgcca ggctttcctg
     1261 ctggcgcttg taatccgtga tgtcattgag ggaaagtaca accccggtga tctcgcccgc
     1321 ggcgtcgcgc agcggagagg cgttggcgga aacgaacacg cggcggccgt cgggctggtc
     1381 gatccacatc tcaacattgc gcaccggttc tcctgtggct acggcgcgat cacatgggtg
     1441 ctgggcagac tggagcaact ctccatccgg ggaacgcgca cggaaacacg gatcattgtg
     1501 ggtccgcttg gtgatttctc cggcaggcag ccccaaaatc ctctccgctg ccgcattggc
     1561 gagcgtgaaa cgaccgtcgc gatccgcgat aagaacgccc tctgccagcg tctgcacgat
     1621 gcgcgacaaa tgctcctcat tctgccgcag gctgcaaaat gccgccgcca gcctgatgat
     1681 agggccgata tgcctgccca gggttctggc gaacaccctc cagccttcat cgagctccct
     1741 tgccgaagaa accagcacca gcgcgccgat tcgctgcgcc cccatcagaa gcggcgcgaa
     1801 aagaaccgtc tcggcccgcg ctaggtcgag ccaatcccgt atgggcccgg cggcggcgaa
     1861 gcgggaaatc agcagtggct cccggctctc tatcgcctgc ccgagcaacg ccagccggcc
     1921 gggaacgctt tcgagaacgg acgcttccag atccgaagat ccccgcttcg cccgcaagca
     1981 gaaccgggcg tcctggtcca gcatgtaagc cagcccccca gaaaggccgg tttcctcgag
     2041 acaacgccgg agcagttcat cgaggagcgc ctcaacggaa agatttttct cgagcgtttc
     2101 cgccaggccg gtcagaatcg cgctttcagc ttcctgcaaa ttgatccggg aaagccaggc
     2161 gccggtgttc ggttgctgct gcggttgcgg tttcaggtgc tcgccctctt ccccggggga
     2221 agaaatcggc tggaggaagc aattccctta cggcgaaccg acctggatct tacggatacg
     2281 taaacgcggc aacacaaccg cagagcggcc gcgcgcagat caagaagcgc tacccgagca
     2341 acgagcgcag gcgccgccgc aagggaggac catgctgcgg atcggccaga gcaaattcga
     2401 tgaaggtttc gaaatagctg cggaagttgc cgatatcgta acgtctctcg ccgccggaaa
     2461 gcttgacgcc gacgatgcgc tcgcccgcaa ccgccagcat tcgaatggca tccgtcaact
     2521 gaatttctcc gcgccggtcg ggctcgacct ttcgaatcat ctcgaaaatg gccggagaaa
     2581 acacatagcg gccggcgata gccaggttgc tcggcgcccc cccgggagcg ggcttctcga
     2641 tcaaatcgcg gatgccgaac acttctccct ccgactcggg agcgaccact ccgtaaagcg
     2701 acgtcagctc gcggggaact tcttccacgg cgatcacgca ggcggcgcgc tcgctctcat
     2761 gcacctccgc gatccgccgc gtgatggtgg aactgccgtt caggccgata atggaatcgc
     2821 cgagggcgac taaaaacggc tcagagccgg caaagttttc cccgcacaaa atagcatccc
     2881 ccaaaccgcg ttgcaggcgc tggcgcgtgt agaaataatg gactttcaac tgttcgaagc
     2941 gcagctcgtc gagaaggccg ttcttttcgg ccttgtttag cgagcgggaa agctccgggt
     3001 cgtgatcgaa atgattttca atcgacgatt tgccgcggcc ggtgataaac agcatccgct
     3061 cgacgcccgc cgcggccagc tcttccacca catattggac gatgggcttt ttggcgaccg
     3121 gcagcatctc cttgggctgc gattttgtgg ccggcagcag gcgcgtgccc agtccggcca
     3181 ccggaatgat ggcgcttttg atcacgataa ttccaggttg tgagtctttg tgctgggccg
     3241 gccatggcat accatgcgga aagccccggc acggccccac aaccccaacc ttggttcata
     3301 ctacaacacg gtgatatttc agaccaccgc cgccgacgcc ggccagcgcc tcgatcaatt
     3361 tctggcgcgt catttcgagg aaaccagccg ctcccggctg caacagtgga tccgagcggg
     3421 ccatgttctg gtgaacggcg cagttgcgaa ggcctccgcc cggttgcaag gaggcgagca
     3481 gatcgaagtg agaccgctcg ctccacccgt ccccgcgcgc gcctttcccc aggagattcc
     3541 tctcgagatt ctctacgaag acgacgatct ggtggcggtg aacaagcccg ccggcatgac
     3601 cgtgcatacc ggcgccggga tcacccaagg cacgctggta aacgcccttc tgcatcgctt
     3661 caagcggctg tcggcggtgg gaggcgaact gcgccccggc attgtacatc gcctggaccg
     3721 ctggaccagc ggcgttctgc tggcagccaa aaccgacgcg gcccacgtcc gcctggggcg
     3781 gcagtttgcc ttgcggcagg tccgaaaaac gtatctggcg ctcgcccacg ggcgcgtctc
     3841 gggctcccgc cagggccggc ccgtggtatt ggacggattc gagtggcggc ggctcgaaat
     3901 gccgatccgc cgggaccgcc gctaccgcgc gaaaatgacc gcgcgagcgc gcgaaggccg
     3961 caccgcgctt accgattatc gcgtgatcga ggagtggccc ggcttctcgc tgcttgaggt
     4021 tcgcatcggc acggggcgca cccaccagat ccgcgtgcat ctctcgacga tcggacatcc
     4081 ggtggccggc gatactcttt atggcgcgcc gcaccagccc accctggaaa ggatttttct
     4141 tcacgctcgc gagctggtat tcacccatcc ctcgaccggc gaaaccctga agattctggc
     4201 gccgcttcct ccggagctcg aatcccaatt gcaggcgctc cgcgaaggga gatccgcgct
     4261 ggtgctgtta taatatgaat actccatgag agccggtttg ctggttctgc tgactttgtg
     4321 gcccctcgcg gcgcaagagt caaggattcc gccaaatcac acgcccgaaa gcaagatcga
     4381 ggtggaggtc ccccgtgtgc cgctgctttt cacggtgacg gacaagaag
//
LOCUS       DASREK010000037        26965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2166687, whole genome shotgun sequence.
ACCESSION   DASREK010000037 DASREK010000000
VERSION     DASREK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 26965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26965
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166687"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2077
                     /locus_tag="VFA54_02290"
     CDS             <1..2077
                     /locus_tag="VFA54_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49660.1"
                     /translation="WQPTSQDSVFFRYSYSTRFRFIPGNFGGIADGTSSSALGRQNLT
                     ATQISLGWTRPINPRLVNELRIGFGRDNSFAEQDPFGLNKVSDYVPGVPDNPAVAGGL
                     PRMTFNGFNTFIGSPDFLPKFQITQQYQWTDTVSVTRGKHQLKFGGDLRAPLRNNFMD
                     IPQTRGFLGFDRIFTCQRNATGCVPGTGLSYADFLLGQVQSAGLTNLYFVDQRLHMFS
                     FFGQDDYKLTPKLTLNLGLRYDYATPALEGKNRIANFNPAGAGSLVFAKNGSISDRAT
                     VNDNRHNFAPRVGLAYQLTSNTVLRAGYGIFYMLFDRIGSEDQLALNPPNLIQNTVSL
                     TSTAPAPVFPLRNGFPTNYLDPANLVLARVKVRGANPDAPNTYVQQWSFGVQRLLPHN
                     LFVDVDYVGTKSTHLNTLRNFNQPINGVLPYPNFGQIEYRDPLGNGIYHGLEGTLERR
                     FSNGMSFRAAYTFSKSIDNDAEHLSVYGSNSFNQDGRNFKAWRGPSDYDVPHRFVASY
                     IYDLPFGQGKRLASHGPLSYIVGGFQVSGSVVAQSGRPFTVFAGNNDSSIDIGLQHAL
                     PNVVGAPQVLQDVNCWFYSSTSTACRALAPNGVDAFSTPPQRVFGNAGRNIIRAPGQV
                     GTDFSLHRNFPLWSESRLLEFRWEVFNLTNTPLFGLPGNNINSGSVAAITTLAGDPRI
                     MQFALRFKF"
     gene            2246..2635
                     /locus_tag="VFA54_02295"
     CDS             2246..2635
                     /locus_tag="VFA54_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49661.1"
                     /translation="MRYLVRAPVKPGCERALLEAIENGTLGQGSVAEGEYLRNMKDAR
                     LCSDQSARWVEICYCPTPLQEERPYWEQYFELAKIQDAHDRRKCRMKTGPRPGPATAV
                     IAQKSWNEDFRPGASLFSKRFAARSPP"
     gene            complement(2859..3437)
                     /gene="pal"
                     /locus_tag="VFA54_02300"
     CDS             complement(2859..3437)
                     /gene="pal"
                     /locus_tag="VFA54_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZS49662.1"
                     /translation="MQSRLLAGLSVAMLSMTIGGCSKKAPPPPPPPPAPSAQASPARA
                     TPAVRQTNQRREQTATSAPSRMPDAATRARIQDLLNRIQDAYFDYDKHDIRPDAEAAL
                     QKDAQTLAEIIRQYPDFKLTVEGHCDERGSEEYNLALGDERAQTAKKYLANLGVPADQ
                     LKTVSYGKERPVCSDHDEACWQKNRRAHITQD"
     gene            3630..4991
                     /locus_tag="VFA54_02305"
     CDS             3630..4991
                     /locus_tag="VFA54_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS49663.1"
                     /translation="MRHRPWLVLVGGFGGLLLLMAAAEVGALFSLNRLRRSDAALQNH
                     FLSRNRALEQIRSNIYLSGTFARDSLLAPEPSGAHAQIAALEGLRQDTEAALDRYSQA
                     LEPEEAAPFGALRSEIDAYWKILNRTFAWTPGERNKNRYAFFYEELVPRRTSMLQIAD
                     RIAALNEEMLKRGNDKLDALFGRLQLGLVGMIVLTLLGGATLAGLTIFHIVRLENEVR
                     KRLEESVRAQAGLQELSAKLVRAQEQERRALARELHDEIGQSFSAILMEVENLLDLQP
                     AGEASPRLESIRSLAEKGVNEIRNMALLLRPSMLDDFGLVPALNWQAREIAKRTGLRV
                     QVASELADGVPEEHKSCIYRVVQEALNNCARHAQATSVQVAVRGETGRITLSVQDDGS
                     GFDPQRVRGLGLLGMEERVRHLGGTFEIDSRPGCGTLLKVALPLATMEGAQESEADSH
                     SSG"
     gene            4963..5613
                     /locus_tag="VFA54_02310"
     CDS             4963..5613
                     /locus_tag="VFA54_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS49664.1"
                     /translation="MKPIRILLADDHNVLREGMRLMLERQPGLAVVAEAADGRETLDL
                     VAEHSPDVVVMDIAMPGMNGIEATRRIIEKHPGTGIVILSMHDDESYVIRSLKAGARA
                     YLLKDALKADLIAAIHAVAEGKSFFSPKISRILQEEYTGALERKGSDDTYELLTDRER
                     EILQLVAEGKTNKEIAAILNVSLYTVDTHRTHILRKLNLHSVPEVILYAVRKGIIS"
     gene            complement(5646..6383)
                     /locus_tag="VFA54_02315"
     CDS             complement(5646..6383)
                     /locus_tag="VFA54_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS49665.1"
                     /translation="MPEAAVQSQDVGEQLRRQGVVIRTYDLWKTYFMGTEEIHAVSGV
                     SIEIKKGEYVAIMGPSGSGKSTLMNLIGCLDTPTAGRYYINGQLVSDLSDDELAHIRN
                     KEIGFVFQTFNLLPRATALHNVELPLIYNGTPAPVRLERARQALLNVELGGRMYHKPN
                     ELSGGQRQRVAIARALVNNPAILLADEPTGNLDSATGNEIMALFERLHDQGNTIVLVT
                     HEHDIAMHARRIIHLRDGKVERDERIR"
     gene            complement(6400..7824)
                     /locus_tag="VFA54_02320"
     CDS             complement(6400..7824)
                     /locus_tag="VFA54_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS49666.1"
                     /translation="MSTGKKLLLTGIGLAALGGIVYGSIRYKQKGITPVQTGRVVRQD
                     LVSQVTASGEIKPKNYINVGAQYMGILTEILVKEGDRVKKGQLLAKLETVQPAADVAA
                     QQATLKTAQADLLAQEAAIKSAQENLKTVQASLERAQADLEKAAVAFDRARQLLASQL
                     IAQQDYDQRKSEYDAAVAGVSEAQARVSQTRAQLEQSMAQRESAVRRIDQLQANLTRL
                     NDVLQKYYSLAPLDGVVTNLPVRVGETVVPGIQNSPASLIMTIADMSEITAEVRVDET
                     DIVNVKLNQPAEITVDAIPGQIFRGRVSEIGTTAIIRSTGLSASQSTVSTQEAKDFKV
                     VIAMENPPEGIRPGLSCTAKITTATRNKVLTIPIQALTVRTAADLAPEAKAGGVQAAD
                     KSTAVVPPSKRREEVQGVFVVSGNQVTFRKVATGVTGASDIEVVDGLQEGEEIVTGSY
                     KVLRTIRPGAAVKVDNKAAGTAVQ"
     gene            complement(8206..9744)
                     /locus_tag="VFA54_02325"
     CDS             complement(8206..9744)
                     /locus_tag="VFA54_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZS49667.1"
                     /translation="MRLCKTKRQNQKGQAIILVLVALGLFLLGGIGLAIDGSHLYAQR
                     QMAQAAADAAAQAAIMSIFNGTNTSGGSPDYKNAFGSSAFDCAANSTLTPCYYARLNG
                     FDPANGDVVHVDFPASDPGTTLSASDPVALCRVTIIRTVNTSLMRFLGPSTSKVAAQG
                     TAAIVDVVAPVPIIVTHPNLSGSFFINGGPTITICGGPQRSIQVNSSSPTAISLSGSS
                     NVVDLSKAGPKDDGFCNSGTGTDFGNFGGPESYPNAGGLLLGSTGKYLDHAAVILDPL
                     KDILATPSQPIITQSNPLPVPNGVDGCPASPAKACALYLPGYYPNGINVKNQTALFAP
                     GLYYIGNGGFGNAANGDMMMATCPAPAVGGLPAIPITGTDPAFGCGMMVYNSGTGVFN
                     VGANSSANLLGPPDGPPYYGVLFFENRNAPENNGKNSGHRFGGGGGMTLIGTIYVNNL
                     LSYQLADPTHYQRLVLQGTPGQQTLIRGEIITNVLELGGNAGIKMQLDPLNKLHIRQV
                     ALVR"
     gene            complement(9757..10227)
                     /locus_tag="VFA54_02330"
     CDS             complement(9757..10227)
                     /locus_tag="VFA54_02330"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZS49668.1"
                     /translation="MKAARISARLPRRGSSLVEFALVALQLSLVIFAGLEFGRMVLVY
                     TNLCNAARVGVRYAIVHGSERTGTGVNGPASSNDICGVAGLGGATTNGVVADYAKAGL
                     LNPKALSCTVSWPDGGLKTAGSRVQVTVTYPYDPFVVLPLRVRLGTTTEGIITF"
     gene            complement(10224..10436)
                     /locus_tag="VFA54_02335"
     CDS             complement(10224..10436)
                     /locus_tag="VFA54_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49669.1"
                     /translation="MATGTFATTPPDVPADTRPEGPLYVVGWVDIDYTYRPFIPLGFQ
                     FPGLGLRLTLPNNLVIHRRAVMRILR"
     gene            complement(10578..10781)
                     /locus_tag="VFA54_02340"
     CDS             complement(10578..10781)
                     /locus_tag="VFA54_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HZS49670.1"
                     /translation="MKTTNRRHNLAGAALVEFALVLPLLFVVFLNAVNFGGFFFAWIT
                     VANAARTAANYMIMGGASVNSPP"
     gene            complement(10778..11170)
                     /locus_tag="VFA54_02345"
     CDS             complement(10778..11170)
                     /locus_tag="VFA54_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZS49671.1"
                     /translation="MQNFRWLKNWIRTLRNGKEPEFVKVPLVASFWNGGVSTQYAVKK
                     VTKTGAYVVTGDKWYPGTIITMSFQYDPYYVQVAQINGHARNSLRMRAKVTRHEPDGV
                     AVRFIYLNDRERRAFEKFLADGTVRGTK"
     gene            complement(11149..11607)
                     /locus_tag="VFA54_02350"
     CDS             complement(11149..11607)
                     /locus_tag="VFA54_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZS49672.1"
                     /translation="MDKKVCVFNQTRQSFLGLEVAVADTHLSRLIGLLGSKKLRPDGG
                     LWVVPSYGIHTIGVLFPIDVVYLDSEHRVIHVIEHLGPFRIAPMRMHAVSVLELPLRT
                     IYESQTEVGDELLICSPLDMEVHWRALQARKLAESQDAVKQGLSCKTSGG"
     gene            complement(11867..12784)
                     /locus_tag="VFA54_02355"
     CDS             complement(11867..12784)
                     /locus_tag="VFA54_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZS49673.1"
                     /translation="MTLALLTFIAIFMLITSGALLLAYREALLNRLSSVVSPASSPQN
                     LLQRIFGARAKASIEEVIDPFQKILPRSPEEVGVIQKRLIWAGYRKDSYVNIFYGAKV
                     AVPLALCVLATVTKAYHYGPFFVYALAGGLGFLLPDFWLGNRIAARQLKIRLGLPEAL
                     DLMVICIEAGLGIDQAVLRVADELKMSQPEIADELNLVNLEQRAGRARADAWRNLAER
                     TDVDSVRSLCAMLIQTDYFGTSIAKSLRVHSESLRTQRRQQAEEQAAKTTVKLVFPLV
                     FCIFPSLFVVVLGPSLIIVFDAFEKYLLH"
     gene            complement(12794..13765)
                     /locus_tag="VFA54_02360"
     CDS             complement(12794..13765)
                     /locus_tag="VFA54_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZS49674.1"
                     /translation="MFLIALLVFIAVFAAVVLAMVAMGAGTNKDQKQILERLESISAA
                     AQRRPDDEGLNILREELLSSIPWVNRLLNEVDLFPKLREILRQADVKWTVTSLLLNCL
                     GAGLAAGAAAYWRTRAIPFALMVGAVAAGGPMAYVYFKRSQRFGKFEEKLPEALDLMV
                     GALRAGHSLISALEMVVREIPEPISGEFKKCFDEQNFGLELREAMINLGNRVPLHDMH
                     IVVTAILIQKESGGNLAEILEKVASVIRDRFRLKRQIRVHTAQGRLTGWILALLPVIL
                     GLGLYFVNPAYMSRLWQDKTGLKLMYTAAGMTIVGALIIRKIIQIRV"
     gene            complement(13794..15074)
                     /locus_tag="VFA54_02365"
     CDS             complement(13794..15074)
                     /locus_tag="VFA54_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZS49675.1"
                     /translation="METLDFQQLKRDVHRTLLSKVDLEKLSAVNNGKAMQAVSALIQE
                     IIAKERIPLSAAEKERLQADLMDEIFGLGPLEPLLKDHTISDILVNRKDMVYIERGGI
                     LQRVNVHFRDDAHLLQIIDRIVGQVGRRIDESSPMVDARLMDGSRVNAIIPPLALDGP
                     ALSIRRFGTEPLTAEQLLQSQTLSPEMLEVLKGAVRARISILISGGTGAGKTTFLNML
                     SKYIPETERLITIEDAAELQIQQENVVRLETRPPNVEGKGTIKQRQLLINSLRMRPDR
                     IIIGEVRGDEAFDMLQAMNTGHEGSMTTIHANTCRDALARLESMVAMANVNLPERAVR
                     QQIASAIGIVLQLSRMSDGTRKVVNIAEITGMDEDVVSMQDIFTFVKKGIGPNGKVVG
                     VFQPSRIRPKFVERIRVSGILLPQDLFERSMEVN"
     gene            complement(15102..16310)
                     /locus_tag="VFA54_02370"
     CDS             complement(15102..16310)
                     /locus_tag="VFA54_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS49676.1"
                     /translation="MNSTFEYEHPPVVKNMLSVALIGPDEKRRRALAAALERHPVTLT
                     GEYGAYPNYHQLAKLGESGCDVVIVDLDPDPEAALDLVENICSRNLAATVMVYSATRD
                     PEVLVRCMRAGAREFLSEPISADTLAEAVIRATARRLEIEKQKKLSGRVLVFCGAKGG
                     AGVTTLASNFAIALKQEAGAEVALIDLKTQLGDVAVVLGLTPHFTIADALHNSERLDQ
                     DFVSSLLVEHRSGVAVLASPDQCSAQTLVMDGSLRKLLHVLRSRFPFVVVDGGSIFTE
                     GLQVACEMADSIYLVTQAEIPALRNAQRLIAHFQQPGMPERRIEMVLNRFDPRKVEIQ
                     ESRMAKVLPLPLKWKVPNDYFSVCRSLNTGAPLALENSSIAKALRQMARQACGKPVEQ
                     GKKKRWGLFE"
     gene            complement(16307..17215)
                     /gene="cpaB"
                     /locus_tag="VFA54_02375"
     CDS             complement(16307..17215)
                     /gene="cpaB"
                     /locus_tag="VFA54_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZS49677.1"
                     /translation="MNRSRLLATFLVALLVAGAASLFVFQKLRQSTARASSGSRVIVA
                     ARDLEIGQKLSEGDLKVVDWPGSSPTGAFSKASDIVGRAVLYPVFDGEVLLQAKLAPE
                     GSGAGLPAVIPAGMRAVSIRVDDVVAVAGFVGPGTRVDVLLTGNPANGRAGGGDESLT
                     KTILENVQVLAAGQKIQPDDQGRPEKVNVVTLLCNPEDAAKVTLAANEGHIQLVLRNP
                     TDTSKVAKEAVVGRQALYGGPAPKPAPAPKPVKRAPRPEPQIAALPPAPPEPPVIMKA
                     VIEVVRGEKVSQVEVGPAASQGGTER"
     gene            complement(17242..17766)
                     /locus_tag="VFA54_02380"
     CDS             complement(17242..17766)
                     /locus_tag="VFA54_02380"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZS49678.1"
                     /translation="MHPVHSIALWPTLIVLVIATISDIRTRRIPNRLVFPFLAAGVTV
                     NGVAQGMRGVAQSAAGIGIAAAVLGVLWYLRGMGMGDLKLFAAVGAWIGPSQLITALV
                     ATAMAGGFLALAYALWNRSLGKCIDGTGELVSGFFRDGFRPHKELRLENAAALKVPYA
                     AAIAIGTMFSFYAL"
     gene            complement(18332..18517)
                     /locus_tag="VFA54_02385"
     CDS             complement(18332..18517)
                     /locus_tag="VFA54_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZS49679.1"
                     /translation="MKNLLLKLYVALQNESGQDLIEYALVVALIAFAATAGMNTVATD
                     INTAFTNIGTKLTTYTS"
     gene            complement(18704..20236)
                     /locus_tag="VFA54_02390"
     CDS             complement(18704..20236)
                     /locus_tag="VFA54_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HZS49680.1"
                     /translation="MLSRRTVESLAILLGVAISRLLFRSHYLYDIDSVNFALALGRFD
                     PAVYQPHPPGYFLYVCLGRLANLLFADANSALVAVSVLASCGAAALIYALADAWFGGE
                     AAVLAGVLFLFSPLSWFHGTVALTYIVEAFFSALIGYLCWQSYSGRTAFLVPSALALG
                     VAAGFRQSSLLLLAPLWLASLRKARRNQVIAGLAALLAALLAWFIPMVRAAGGIAAYL
                     APLQDLWHVVLAKHTFLRSPLALSVARFCTILGIYSLLAGAAAWLPLAARNAGEAREK
                     KQFTWIWIAPGLLFFTFVFLRFVNSGYLLVLSPPVFVWLGRWAARLYAAWNAPRRRKL
                     ALTAAVAAANTAVFLYAPVYCSYKSLKDFEAELVGVNRSIRAACSPRDTVIIGFDSHF
                     LGYRHAAYYLPEYLTVQFPEVEFAAGKRVFAVQDRETRAESELALAALPHFVFYPLPA
                     GPEYSQYLEDLRRRLPPGSLQAVRAGGRDLLVGPTADLALLFPATLSLAAQPYTAHDG
                     AR"
     gene            20326..21480
                     /locus_tag="VFA54_02395"
     CDS             20326..21480
                     /locus_tag="VFA54_02395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZS49681.1"
                     /translation="MPRRLAQIGAWLLAALLAGLLLLPLRSQPPWDFLQFYVAGKLAA
                     SGRVAEIYHKPAYPPLVDEIRSSESRSEISSAYFNRTPYFNRPAFAAYLCAPFARLPF
                     QTAAHLWILANFALLAALVWKLPAWLPAGAPLRPWLAGFLPWIWSIGLGQDTLLISLL
                     AAYSLHLALQKREIPAGLVLAGCAFKPHLLAAMPFALLAARKRKMAAAFLAAGAALAA
                     ISAAVAGSTGFREWMELLHSRTTDSMPETMGNIRALALHFGFASGLAAGAVAAGCFLF
                     VLRQGRLRQSFAAAILAALLLGPHTYLQDYSLLAVVALSAFDRWAQYALLLPWPYFYR
                     REDMLPLTLVALAGLIVLAARADTALAPDTAQTCAGEGGADARNSACAAV"
     gene            21449..22633
                     /locus_tag="VFA54_02400"
     CDS             21449..22633
                     /locus_tag="VFA54_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZS49682.1"
                     /translation="MLETALAPQSEAGCRLDAFSGKALLGIAWLAAVWIFLVSLAPAF
                     SLMNPSHSGYFPPDYMQFYMVGRMAAAGDIAKIYDKAAYEPLVRELRSQGERIGDYPF
                     NRPSFEAFACIPLSWFSYRTALLFAFAGNLAVFGLLLWKLPIWFGAPAWTRPCLLVFT
                     PFLYNIALGQDVLPLALLVASTLRQALLGRDRLAGILLGLCCFKPHLVWLLPMAPLVA
                     GRRRMFWSFAATAGALAALSIAALGTAGLRDWILLLRSPFSDYMPETMCNWRGLARHL
                     GPAPAAAAAIFILGSVVIILCRASDAEKVLAALVASLLLSPHTYHYDYALLAVAAMAT
                     PLPAVRILLLLPWSYFHELTNPLPWIALAIVWLGSTALPLLRRRVVGEMNRTESLAPA
                     GF"
     gene            22687..23952
                     /locus_tag="VFA54_02405"
     CDS             22687..23952
                     /locus_tag="VFA54_02405"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZS49683.1"
                     /translation="MAGTLARYALAAFAAGALLAGALRCKTGAQPLLASQLAGAFLIY
                     AVKRPGARSLLRAAAGALAFAVGYALLRGKWPGAFVYWPLVMGAFLGLGGLLALLVEL
                     FWSAPGSAARREVANSLFAVGVLPLMLIATFLAHGVTRHLHPMTYDRFLYAFDGALGC
                     QPGYSLGRLFAASPPLAVVSNLVYVTLPLPAALVYISHLKAREGSPELVLRVFLGIGV
                     AGFFMYQICPATGPVYAFGHFPSNPPVFSLSDLVPIPIRGGFLNAMPSLHVAWALALL
                     WNCRPGQAGWRAAAGVYLFFTILATLGTGEHYLADLVAAFPFTLAIQAACTTAAELRS
                     RESRRALCFGGAAALAWPIALRTSLPSLLSYPRLSWILAGATVIAAVILAWPLLQAQS
                     SPAPKPGAAPEYAGAIGASEGKFPCCSPS"
     gene            23934..24968
                     /locus_tag="VFA54_02410"
     CDS             23934..24968
                     /locus_tag="VFA54_02410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS49684.1"
                     /translation="MLLSIVVPVYNEAESLPRLLEALREVLPQTGCEHELIFVNDGSR
                     DGSFEVLSQAARSDPRVKILGLSRNFGHQAAITAGIDFAAGDAVAVMDADLQDPPELL
                     PDMVDLFRHGYDVVSAQRVARDCDSFFKRATASLFYRFMRKAIDSRLAPQVGDFRLFS
                     RRAVAALRGFREQHRFMRGLVAWLGLKEAILPFHRQPRAAGETKYSLCKMLRFAWTAI
                     SSFSALPLRLCLTFGLLMTLGGFLYFAWVLYVTVVLKGTVPGWTSLVGLQVIFSGSTL
                     VAIGLVGDYIARIYEEAKGRPLYVVSEAVNFSHAERHLERMVLLPAPEERHDANSAEG
                     TSSQPAASRI"
     gene            24913..25638
                     /locus_tag="VFA54_02415"
     CDS             24913..25638
                     /locus_tag="VFA54_02415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS49685.1"
                     /translation="MTQTALKERPLSRPPAEFDSYVEAYPELLRDPVRERFAPGGRFF
                     IERKRLLLKAFLQGAGVAMQRARWLDIGCGRGDLLAASQSEFRYAAGCDLSAGMIAGA
                     KGLDVRLQNAPDQLPFDDGSFDLLTAVCVYHHVDVEVRARLTTEAARVLRPGGIFCVM
                     EHNPFNPVTRFIVRRSPVDANAELLSAGEVRRLVRVAGMQVLRTQYFLLFPEFVYQRL
                     AWLEAGLSGLPLGGQYAVFGSRP"
     gene            25653..>26965
                     /locus_tag="VFA54_02420"
     CDS             25653..>26965
                     /locus_tag="VFA54_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49686.1"
                     /translation="MSLLAIETALTVITAGLAFAGPRLGSRWFRRWEDAFARLARRKG
                     LSIVVVGLAALLARLALLPALPIPEPGVHDEFSHLLAAETFASGRLANPPHPMWVHFE
                     SFHIDQLPTYMSMYPPGQGLILAAGKWIFGHPWFGVWISTGAMCAAFGWMLQGWLPPG
                     WALLGGFLAVLRLGIFSYWMNSYWGGAAPALGGALILGALPRLLRSPRAGHAALLALG
                     LAILANTRPYEGLVLSVPVLLALLVAAIMGEPRRARLLRRAALPAGAILLAAAAAMSY
                     YNWRVFGNPLTLPYQINRATYAVAPYFPWQTPRPEPAYRHRVLRDFYVGYEFADFEKT
                     RTPGGFLSRTREKLTTVFSFYFGPALALPLAFLPRALRDRRLRLLALAGAATALGLAA
                     EVWLIPHYAAPATCLLYALLLQAMRHLRAWRPEGRFLVRGLVMVCV"
BASE COUNT         5013 a         8433 c         8122 g         5397 t
ORIGIN      
        1 ctggcagccg acttcccagg actctgtctt cttccgctac agttactcca cgcggttccg
       61 gttcattccc ggcaattttg gaggcatcgc cgacggcaca tcctcttccg cgctcggccg
      121 ccagaatctc acagcaaccc agatctcgct cggctggacc cgtcccatca acccgcgcct
      181 ggtgaatgag cttcgcatcg gcttcggccg cgacaactcg ttcgccgaac aagatccgtt
      241 tgggctgaat aaggtctccg attacgtgcc gggcgtgccg gataacccgg cagtggccgg
      301 cgggctgccg cggatgacgt tcaacggctt caatacgttc atcggctcgc ccgatttcct
      361 ccccaaattc cagatcacgc agcagtatca gtggaccgac accgtttcgg tgacgcgcgg
      421 caaacaccag ctcaagttcg gcggcgatct gcgggcgcct ctgcgcaaca acttcatgga
      481 catcccgcaa acccgcggct tcctgggctt cgaccgcatc ttcacctgcc agcgcaacgc
      541 caccggctgc gtgccgggca ccgggctttc ctatgccgat tttctgctgg ggcaggtgca
      601 gtcggcgggc ctcaccaatc tgtacttcgt ggaccagcgc ctgcacatgt tttccttctt
      661 cggccaggac gattacaagc tgaccccgaa gcttacgttg aacctcggcc tgcgctacga
      721 ttacgccacg ccggccctcg agggcaagaa ccgcatcgcc aacttcaatc ccgccggcgc
      781 cgggtcgctg gtcttcgcga agaacggatc tatttcggac cgcgcaacag tcaacgacaa
      841 ccggcacaac ttcgctcccc gggtcggcct ggcctaccag ctcacatcca acaccgtcct
      901 gcgagcaggc tatggcattt tctacatgct gtttgaccgg atcgggagcg aggaccaact
      961 ggcgctcaat ccgccgaacc tgatccagaa cactgtgtcg ctcacttcga cggcgcccgc
     1021 gccggtcttc cctcttcgca atggattccc gaccaattat ctggaccccg ctaatctggt
     1081 tctggcccgc gttaaggtac gcggggcaaa tccggatgcg ccgaacacgt atgtgcagca
     1141 gtggagcttc ggggtgcaga gattgctgcc gcacaacctg tttgtggacg tcgattacgt
     1201 cgggaccaag agcacgcact tgaacacgct ccgcaacttc aaccagccga tcaacggggt
     1261 gctgccctat ccgaatttcg gccagatcga atatcgagac ccgctcggca acgggattta
     1321 tcacggcctg gaaggaaccc tcgaacgccg cttcagcaat ggcatgagct tccgggcggc
     1381 atacacgttt tcgaaatcga tcgataacga cgcggagcac ctctccgtgt acgggtccaa
     1441 cagcttcaat caggatggcc gcaatttcaa ggcctggcgc ggcccgagcg actacgacgt
     1501 gccgcaccgg ttcgtagcca gctacattta tgacctgccc tttggccagg gcaaaaggct
     1561 cgcttcccac ggccctctgt cgtatatcgt gggcggcttc caggtgagcg gctccgtggt
     1621 ggcgcaaagc ggcaggccgt tcactgtttt cgccggcaac aacgacagct cgattgacat
     1681 cggcctccag cacgccctgc ccaacgtggt cggcgcccct caggtgctcc aagacgtcaa
     1741 ttgctggttc tactcctcca ccagcacagc ttgccgggcg ctggcgccca acggcgtgga
     1801 cgctttttcg actccgccgc agagggtctt cggcaacgcc ggccgcaaca tcattcgggc
     1861 tccgggccag gtgggaaccg acttctcgct gcaccgcaat tttccgctct ggtccgagtc
     1921 caggctgctg gagttccggt gggaggtttt caacctgacc aatacgccgc tctttggcct
     1981 gccggggaac aacatcaaca gtggctcggt cgctgctatt acgaccctgg ccggcgatcc
     2041 aaggatcatg cagttcgcct tgcggtttaa attctaactg ccagcgcgtc tcgccgcggc
     2101 ccggaaggag caggaaagcg aagaagcgcc gttccgggct gcacgccttt tggaatcctg
     2161 gccccggatg cgggagcagt ttccatcccc ggttcaataa aagcgggagc agtttccgtc
     2221 cccggttcaa taaaatggga gtgtcatgcg atatctggtt cgggcgccgg tcaagcctgg
     2281 ctgcgagcga gcgctcctcg aagccatcga aaacggaact ctgggccaag gatcagtggc
     2341 agagggagag tacctgcgga atatgaagga tgctcggctg tgcagcgacc agagcgcccg
     2401 ctgggtcgag atttgttatt gtccgacgcc gctccaggaa gagcgtccct attgggaaca
     2461 gtattttgaa ctggctaaga tccaggatgc ccacgaccga cgcaaatgcc ggatgaaaac
     2521 ggggccgagg cctgggcctg ccacggctgt gattgcacag aaaagctgga acgaagactt
     2581 cagacctggg gcgagccttt tctccaagcg cttcgccgcg cggtcgcctc cttagccagt
     2641 cccaacctgt agtcccatca gccgtccaaa tacgctgaga acaaggcggc cgcaggcgaa
     2701 tcgcggcggc agaccttcct tccccacact tcccggaaag cttgacgctc gtgcggggaa
     2761 tgggaggtga gagccgcaag ggcgcccgga ggattccgga cggaccctcg cagcccggga
     2821 gaaagcagtt cgccagatga gccggccggc gccggcatct aatcctgagt gatgtgggcg
     2881 cggcggtttt tctgccagca ggcctcgtcg tgatcggaac acacgggtcg ttctttgccg
     2941 tagctcacgg ttttcaattg atcggcggga acgcccaggt tggcgaggta tttctttgcc
     3001 gtctgggcgc gctcatcgcc gagagcgagg ttgtattcct cggaaccacg ttcatcgcag
     3061 tgcccctcaa ccgtcagttt gaaatccggg tactggcgaa tgatctcggc gagcgtctgc
     3121 gcgtctttct gcaaggccgc ttctgcgtcg ggccggatat catgcttatc gtagtcaaaa
     3181 taggcgtcct gaatccggtt caacaggtct tgtatgcgcg ctcgggtagc ggcatccggc
     3241 attcgggaag gcgcgctggt cgctgtttgc tctcgccgct ggttggtctg ccgcacggcc
     3301 ggagttgccc gagcggggct ggcctgcgcg ctcggagcgg gcgggggcgg cggtggcggc
     3361 ggcgccttct tggaacaccc gccgatcgtc attgagagca tcgccacgct caggccggcc
     3421 aaaagtcgcg attgcattag aaagtcctcc atacgagaaa tcttcgattc accatatacg
     3481 gaaacaggct cgctgcataa tcccgtcgat acggtatttt ccagatccaa acgataccgg
     3541 atcaacggga ttccccaaaa tacctcagag gattatctta gagggtttag ctggcatgct
     3601 tcgcaatgcc agccccggca gggggaagga tgcggcatcg gccctggctt gtactggtgg
     3661 gcggatttgg cggcttgttg ttgctgatgg cggcagccga ggtcggagcc ttattttccc
     3721 tgaaccgcct gcggcgcagc gacgcggcct tgcaaaacca cttcctttcg agaaaccggg
     3781 cgctggaaca gattcgctcg aatatctatc tctccggcac tttcgcccgg gattctctgc
     3841 tcgcccccga accttccggg gcgcacgcgc agatcgcagc gctggagggg ctgagacagg
     3901 acacagaagc ggcgctcgac cgctactcgc aggccctcga gcccgaagaa gcggctccct
     3961 tcggggcgct tcgctcggaa atcgacgcct attggaaaat tttgaaccgt acttttgcct
     4021 ggacgccggg agagcggaac aagaaccgct atgccttttt ctacgaagag ctcgtgccgc
     4081 ggcggacgtc gatgctccag atcgccgacc gcatcgccgc gctgaatgaa gaaatgctga
     4141 agcgaggaaa cgacaagctg gacgcgcttt tcggacgcct ccagcttgga ctggtgggca
     4201 tgatcgtttt gacgctgctc ggcggggcga ctttagccgg attgaccatt tttcatatcg
     4261 tgcggctcga aaacgaagta cggaagcggc tggaagaaag cgtgcgggct caggccggcc
     4321 ttcaggagct ctcggccaaa ctggttcgag cccaggaaca ggagcgccgc gcgctggcgc
     4381 gcgaactgca cgacgaaatc ggccaatcct tttcggccat tctgatggaa gtggagaatc
     4441 ttcttgacct ccagccggct ggagaggctt ccccgcgcct ggagtccatc cgcagcctcg
     4501 ccgaaaaggg agtgaacgaa atccgcaaca tggctctcct gttgcgccct tccatgctgg
     4561 acgattttgg gctggtgccg gctttgaact ggcaagcgcg cgagatcgcc aagcggaccg
     4621 gcttgcgcgt acaggtggcc agcgaactcg ccgacggcgt gccggaggag cacaaatcgt
     4681 gcatctatcg ggttgtccag gaggcgctga ataattgcgc ccgccacgcg caggccactt
     4741 cggtgcaggt cgcggtgcgc ggcgagacag gaagaatcac gctcagcgtg caggatgatg
     4801 gaagcggatt cgacccgcag cgcgttcggg gcctcggatt gcttggaatg gaggagcgcg
     4861 tccgccatct gggagggact ttcgagatcg attcacgtcc cggatgcgga acgctgctga
     4921 aagtcgcgct gccgctggcc actatggagg gagcccaaga aagtgaagcc gattcgcatt
     4981 cttctggctg acgaccacaa cgtgctccgc gagggcatgc gcctgatgct cgagcggcag
     5041 ccggggctgg ccgtggtagc cgaagccgct gatggccgcg aaacgctcga tttggtggcg
     5101 gagcattctc ccgatgtcgt agtgatggac atcgccatgc ccggcatgaa cggcattgag
     5161 gccacgcggc gcatcattga aaagcacccc ggcaccggga tcgttattct gagcatgcac
     5221 gacgatgaaa gctacgtgat ccgctcgctg aaagccggag cccgggccta tttgctaaaa
     5281 gatgccttga aagcggatct gatcgcggcg attcacgcgg tggccgaagg caaatcgttt
     5341 ttcagcccca agatcagccg cattttgcaa gaggagtaca ccggagctct cgaacgcaag
     5401 ggatcggacg acacttacga gctcctcacc gaccgcgaac gcgaaatcct acagcttgta
     5461 gcagaaggga aaaccaacaa agagatcgcc gccattttga atgtcagtct gtatacggtg
     5521 gatacccacc gcacccacat tctgcggaag ttgaatttgc acagcgttcc tgaagtgatc
     5581 ctctatgccg tacgaaaggg aattatctcc tagcccgggg gggttagtgg agggccggtc
     5641 gcgggctacc ggatccgctc gtcgcgctcg actttgccgt cgcgcagatg aatgatgcgc
     5701 cgggcgtgca tcgcgatatc gtgctcgtgg gtcaccagca cgatggtatt tccctggtca
     5761 tgcagccgct cgaaaagcgc catgatctcg ttgccggtcg ctgaatcgag gttgccggtc
     5821 ggctcatcag cgagcagaat cgccggatta ttaaccagcg cccgggcgat ggccacgcgc
     5881 tggcgctggc cgcccgacag ttcgttcggt ttgtggtaca tgcgcccgcc cagttctacg
     5941 ttcagaagcg cctgcctggc gcgctcgagg cgcaccggtg cgggcgttcc gttgtaaata
     6001 agaggcagct ccacattatg cagcgccgtg gcgcgcggca ataggttgaa ggtctggaag
     6061 acaaaaccaa tttccttgtt tcggatgtgc gccagctcgt cgtcggaaag atcgcttacc
     6121 agttggccgt tgatgtaata ccgcccggcg gtcggcgtat ccaggcagcc gatcaggttc
     6181 atgagcgtgg attttccaga accggaaggc cccatgatgg cgacgtactc gcctttcttg
     6241 atctcgatcg agacgccgga gacagcgtga atctcctccg ttcccatgaa gtaggtcttc
     6301 cagagatcat atgtgcggat tacgacgccc tgccgccgca actgctctcc gacatcctgg
     6361 ctttgaaccg ccgcttctgg catagtgcga tcccgtctcc tattggacgg cagtcccagc
     6421 cgctttgtta tcgactttca ccgccgctcc ggggcggatc gtccgaagca ccttgtacga
     6481 gccggtcacg atctcctccc cttcttgcaa gccatccacc acttcgatat ccgacgcccc
     6541 cgtaacgccg gtggccacct tccggaaggt gacctgattg ccggagacaa cgaagacgcc
     6601 ctgcacttct tcccttcttt ttgacggagg aacgacggca gtgcttttat ccgccgcctg
     6661 gacgccgcct gctttggcct ccggcgccag atcggccgca gtgcgcacgg tcaacgcctg
     6721 aattggtatg gtcagcactt tattccgtgt ggccgtggtg atcttggccg tgcaggagag
     6781 cccgggccga atcccctcgg gcggattttc catcgcaatc acaaccttga aatctttggc
     6841 ttcctgcgta gagacggtgc tttgcgacgc cgataagccc gtggagcgga tgatcgcagt
     6901 ggtgccgatt tcgcttaccc ggcccctgaa gatttggccc ggaatcgcat cgacggtgat
     6961 ttcagccggc tggttcagct tgacgttgac gatgtcggtc tcgtccaccc ttacttccgc
     7021 ggtgatttcc gacatatccg cgatggtcat gatcaggctg gccggggagt tctggatgcc
     7081 gggtaccacg gtctccccca cgcgcaccgg caggttggtg actacgccgt cgagcggcgc
     7141 cagagagtag tatttctgga gcacatcgtt gagccgggtc aggttggcct gaagctgatc
     7201 gattcttcga acggccgact cccgctgagc catgctctgc tcaagctgcg cgcgtgtttg
     7261 cgagacgcgg gcttgcgcct cggacacccc ggctaccgcg gcatcgtact cggatttgcg
     7321 ttggtcgtaa tcctgctgtg cgatgagctg gctggccagc agttgcctgg cccggtcaaa
     7381 cgccaccgcc gctttttcca gatcggcctg ggcccgctcg agcgacgcct ggactgtttt
     7441 caaattttcc tgcgccgatt tgatggccgc ttcctgggcc agcaaatcgg cctgggccgt
     7501 cttcagggtg gcctgctgcg ccgcgacgtc ggccgccggc tgtacagtct ccagtttggc
     7561 gagtaattgg ccctttttta cgcggtcgcc ctcttttacg agaatctcgg tcaaaattcc
     7621 catgtactgc gcgccaacat taatgtaatt tttgggtttg atctccccgg aagccgtgac
     7681 ctgagaaacc aagtcctgcc ggacaacccg tccggtttga accggagtga ttcccttttg
     7741 tttgtaacgg atgctgccgt aaacgatgcc gcccagcgcg gccaaaccga tcccggtcag
     7801 cagcagcttc ttgcctgtgc tcatgcagct tctccaggcc ggcagcgcct cggaaatgct
     7861 gatacgtagg tactacgctg ctggcgaggc gaggcgccgt tttcctccga cgcaggaagc
     7921 gaaaagcgcg gtgcggctcg ctgcggggat cgaagccgcc ggactccggc gcaggctcgt
     7981 ttctgctggt tttggcagct caaaaggaaa aaaacggaac tcttacccaa aagttcccag
     8041 tacccacccg ctattttatc atgggaatat ttcggaggcc gaagaacaaa cgggctgtct
     8101 gtgcgttctt ggcctgaaga ggccgcgagc gggcgccgca cacagccgtc gaagctccga
     8161 ggcgccaagt aaggtaaaac aaaacgctcc gagcgaggcc ctctttcagc gcaccagcgc
     8221 cacttgccgg atatggagct tgttcagtgg atcgagctgc attttaatgc ccgcattgcc
     8281 gcctagctca agaacgttgg taatgatctc gccgcgaatc agcgtctgct ggcccggcgt
     8341 cccttgcagc accagccgct ggtagtgagt cggatctgcc aactgatagc tcagcaagtt
     8401 attcacgtat atcgttccaa tcagggtcat gcccccgcct ccgccgaaac ggtggccgct
     8461 gtttttcccg ttgttttcag gagcgttccg gttttcgaag aacagaacgc cgtagtaagg
     8521 gggcccatcc ggcgggccca aaagattggc gctgctgttg gccccgacgt tgaatacgcc
     8581 ggtcccgcta ttgtaaacca tcatgccgca tccgaaggcg ggatcggttc ccgtaatcgg
     8641 gattgcgggc aatcctccaa cagcaggcgc cgggcatgtc gccatcatca tgtcgccgtt
     8701 tgcagcattg ccgaagccgc cgttcccaat gtagtagagg ccaggagcga acagcgccgt
     8761 ctggttcttg acgttgatac cgttcggata atacccgggc aaatacagtg cgcaagcctt
     8821 cgccggggag gctgggcagc cgtcaacgcc gtttggaacc ggaaggggat tactttgggt
     8881 gatgattggc tggctcggag tggccaggat atccttcaga gggtcgagaa tgacagccgc
     8941 atggtccagg tacttgccag tcgatcccag caacaggcct cccgcgttgg gatacgattc
     9001 cggtcctcca aaattcccga aatcggtgcc cgtcccgctg ttgcaaaagc catcatcctt
     9061 gggcccagcc ttagaaagat ccaccacgtt gctgctgccg ctcaggctga tcgccgtggg
     9121 gctgctcgag ttcacctgaa tgcttctttg cggccctccg catatggtga tggtggggcc
     9181 gccgttgatg aaaaatgatc cgctgagatt ggggtgagtg actataatcg gcacgggagc
     9241 caccacgtcc acaatcgctg ccgtcccctg agcggccact ttgctcgtgg acggccccag
     9301 aaaacgcatc aggcttgtat tgacggttcg gatgattgtg acgcggcaca aggccacagg
     9361 gtcgctggcg gacaaggttg ttcccggatc cgaggcggga aaatcaacgt gtacgacatc
     9421 cccattggcc gggtcaaagc cgttgagccg cgcatagtag cagggagtca gggttgagtt
     9481 ggcagcacag tcgaaggctg acgagccgaa agcattcttg taatctgggc tgccgccgga
     9541 ggtgttggtt ccgttgaaga tgctcatgat ggcggcttgg gcggcggcgt ctgcggcagc
     9601 ctgggccatc tggcgttgcg catataggtg tgagccgtcg atagccagcc caatacctcc
     9661 cagcaagaac aagccgagcg cgacaagaac cagaatgatc gcctgtccct tctgattttg
     9721 cctcttcgtt ttgcatagcc gcataattcg gaacctctaa aacgtaataa tcccttcggt
     9781 agtggtaccg agcctgaccc tcaagggaag gaccacgaac ggatcgtatg gataagtcac
     9841 cgtgacctgg acccgggagc ccgctgtttt caggccgccg tccggccaag acaccgtgca
     9901 ggaaagtgct ttcgggttga ggaggcctgc cttggcgtaa tcggcgacga ctccgtttgt
     9961 agtcgcgccg cccaggccgg ccacgccgca aatgtcattc gagctcgccg gcccgttaac
    10021 gccggtccct gtgcgctcgc tgccatggac aatcgcgtag cgcactccga ctcgggccgc
    10081 attgcaaaga ttggtgtaga ccagcaccat ccggccgaac tccagaccgg cgaagatcac
    10141 caaagaaagc tgaagcgcga ccaatgcgaa ctctacaagg ctgctcccgc ggcgcggcag
    10201 ccgggcggaa atcctggcgg ctttcatcgc agaatcctca ttacggcccg ccggtggatc
    10261 accagattgt tcggcagggt cagtctcaag ccaagcccag gaaactggaa gccgagaggg
    10321 ataaatggcc tataggtgta gtcgatatct acccagccca ccacatagag gggcccttcg
    10381 ggcctggtat ccgccggcac gtccggcgga gtggtcgcaa atgttcccgt ggccacggtg
    10441 cagacaggag tagcggcggt tccgctcggc cccggcccgc tcggacttct ggtgcagacc
    10501 cgtacggcga ggctcgatct gttgagcagg gaagaaatgt cagtggtaac aacggcgctg
    10561 atttgagctg cggtggctta cgggggggaa ttgaccgaag cgccgcccat aatcatgtaa
    10621 ttggctgcgg tgcgggcagc atttgccacg gtgatccagg cgaaaaagaa gccgccaaaa
    10681 ttcaccgcgt tcagaaaaac cacaaagagc agcggcagca caagcgcaaa ctcgaccaaa
    10741 gcggcgccgg caaggttgtg ccgtcggttc gtcgtcttca tttcgtgcct cgcaccgtcc
    10801 cgtcggctaa gaacttctcg aacgcacgcc gctccctgtc gttcaggtaa atgaaacgga
    10861 cggccacgcc gtccggttcg tggcgagtca ctttcgcccg catgcggagc gagttcctgg
    10921 catggccatt aatctgagcg acttgcacgt aataggggtc gtattgaaaa ctcatcgtaa
    10981 tgatggttcc gggataccat ttatcgccgg tgacgacata cgcccccgtt ttcgtcacct
    11041 ttttgaccgc atactgcgtg gacacgccgc cattccaaaa agacgcaacc agaggcacct
    11101 tcacaaactc cggctccttc ccgttcctta aagtccgaat ccagttcttc agccacctga
    11161 agttttgcat gacagccctt gcttgacagc atcctggctt tcggcgagct tgcgggcttg
    11221 caacgcccgc caatgcacct ccatatcgag gggcgagcaa atcagcagct cgtctccgac
    11281 ctcggtttgc gattcataaa tcgtccgcaa aggcagttcg agcacgctga cagcgtgcat
    11341 tcgcattgga gcaatccgga acgggccgag atgctcaatc acgtgaatga cacggtgctc
    11401 gctatccagg tacacgacgt caattggaaa cagcactccg atcgtgtgaa tgccgtaaga
    11461 ggggaccacc cacagcccgc cgtcgggccg cagcttctta ctgccgagca agccaatgag
    11521 gcgtgagagg tgggtatcag ccaccgctac ctccaggccc aggaacgact gccgggtttg
    11581 gttgaaaacg cagactttct tatccacgtt agtccttttc gcgcggccag tgcgagacct
    11641 tcgcgacttc agtcctgccg gccacaagca gattgacccc gccggccgga gagagctccg
    11701 ccgcggcggc tggcgaggtc cgaatgatcg tcctgtccgt ttaacgcacc cgcttgcggc
    11761 gccgctggat cagaacgaac agaacgatga caaagcaaat ccccgaaacc aaccgaatca
    11821 gctctacatt tcccatattc attctcctta agtgagtttt tctgacttag tgcaacaaat
    11881 acttctcgaa cgcgtcgaag acaatgatca gggacggacc cagcaccacc acaaacaggg
    11941 agggaaagat gcagaagacc agaggaaaca caagtttcac cgtggtcttc gccgcctgct
    12001 cctcggcctg ctggcggcgc tgcgtccgga gactttccga atgaacccgc agcgacttcg
    12061 cgatgctggt gccgaaatag tcagtttgaa tcagcatcgc gcagagagac cggacgctat
    12121 ccacatcggt gcgctcggcc agatttctcc aggcgtcggc gcgcgcccgc ccggcgcgct
    12181 gctcgagatt caccagattc agttcatctg caatttccgg ctggctcatt ttcagctcgt
    12241 cggccacccg gagcacggcc tggtcgatgc ccaatcccgc ctcgatgcaa atcaccatca
    12301 aatcgagggc ctcgggaagg cccagccgga ttttcaactg ccgcgctgca atgcgattgc
    12361 cgagccaaaa atcggggagc aggaaaccca agccgccggc cagcgcgtaa acgaaaaaag
    12421 ggccatagtg atacgccttg gtgaccgtgg ccaggacgca aagcgccaaa ggaacagcca
    12481 cctttgcacc atagaaaatg ttgacgtagg aatccttccg gtatccggcc caaatgagac
    12541 gtttttggat cacgcccact tcttcgggac tgcgcgggag aatcttttga aacgggtcga
    12601 tcacttcctc gatcgaggct ttggccctgg cgccgaagat ccgctggagc aggttctgag
    12661 gcgaactggc gggcgaaacc acgctcgaca gccggtttag caatgcctcc cggtaggcga
    12721 gcagcagagc gccgctggtg atcagcatga aaatcgcgat gaatgtcagt aaagcaagcg
    12781 tcataaccgg gcctcagaca cgaatttgga tgatcttccg gatgatcaaa gcgccgacga
    12841 tcgtcatgcc tgctgcggta tacatcagct tcaggccggt tttgtcctgc cataaccggc
    12901 tcatatacgc cgggttgacg aaatacaatc ccaagccgag aatcacaggc agcaacgcca
    12961 ggatccatcc ggtcaaccgc ccctgcgccg tgtgaacgcg aatctgcctc ttcaaacgga
    13021 agcggtctcg tatcacggaa gcaacctttt ccagaatctc ggccagattg ccgccgcttt
    13081 ctttctggat cagaatcgcg gtgaccacta tgtgcatgtc atgcagcgga acgcggtttc
    13141 caaggttaat cattgcctcg cgcagctcca aaccgaagtt ctgctcatca aaacacttct
    13201 tgaactcgcc tgagatcggc tcggggatct ccctcaccac catttccaag gcggagatca
    13261 ggctgtggcc ggcccgtagc gctcccacca taagatccag cgcttccggc agcttttctt
    13321 caaacttgcc gaatctctgg gaacgcttga agtagacgta agccatgggc ccccccgcgg
    13381 ccaccgcccc aaccatgagg gcgaaaggaa tcgcccgcgt cctccagtaa gcggccgcgc
    13441 ccgccgccaa acctgcgcca agacagttga gcaacaggct ggttaccgtc catttcacat
    13501 cggcctggcg caggatctcc cgcaacttgg ggaacaggtc gacctcattc agcagccggt
    13561 tcacccacgg gatcgagctc agcagctcct cgcgcagaat gttcaagccc tcgtcgtcgg
    13621 gccgccgctg cgccgcagcg ctgatggact ccaagcgttc taggatctgc ttctggtcct
    13681 tgttggtccc tgcgcccatg gccaccatgg cgagaacaac ggcggcgaat acggcgataa
    13741 ataccaatag tgcgatcaaa aacatgcttc acctgtctct ttcgcagcgc gggtcagttg
    13801 acctccatcg accgctcgaa cagatcctga ggcagaagaa tgcccgagac gcgaatccgc
    13861 tcgacgaact tcgggcggat gcggctgggc tggaagacgc ccaccacctt gccgttcggc
    13921 ccgatcccct ttttcacgaa cgtaaaaatg tcctgcatgc tgacgacgtc ctcatccatg
    13981 ccggtgattt cggcgatgtt gacgaccttg cgcgtgccgt cgctcatgcg cgaaagctgg
    14041 aggacgatgc cgatggcgga ggcgatctgc tggcgcacgg cccgctccgg caggttgacg
    14101 ttggccattg ccaccatcga ttcgagcctc gccagcgcat cgcggcaagt gttggcgtgg
    14161 atcgtggtca tcgatccctc gtggccggtg ttcatggcct ggagcatgtc gaaagcttcg
    14221 tcgccgcgca cctcgccgat gatgatgcgg tccgggcgca tgcggaggct gttgataagc
    14281 agttgccgct gcttgattgt gcccttgccc tccacgttgg gaggccgcgt ttccaggcgg
    14341 accacatttt cctgctggat ttgcagctcg gcggcgtcct cgatggtgat cagacgttcg
    14401 gtttccggaa tgtacttgga gagcatattg aggaacgtgg ttttcccggc tccggtgcct
    14461 ccggaaatca aaatgctgat gcgggcgcgc accgcgcctt tgagtacttc cagcatctcc
    14521 ggagaaaggg tttgcgattg gagcaattgc tccgccgtca gcggctcggt tccgaaacgc
    14581 cggatggaga gcgccgggcc gtccagcgcc agcggcggaa tgatcgcgtt gacgcgggat
    14641 ccgtccatca gccgcgcatc gaccatcggc gaggattcgt cgatgcgccg tcccacctgg
    14701 cccacaatgc gatcgatgat ttgcagcagg tgcgcgtcgt cccggaagtg cacgttcaca
    14761 cgctgcaaaa tgccgccgcg ttcgatgtaa accatgtcct tgcggttgac cagaatgtcg
    14821 gaaatcgtgt ggtccttcag aagcggctcg agcggcccca ggccgaaaat ttcgtccatc
    14881 agatcggctt gcagcctctc tttttcggcg gcgctcaaag ggatccgttc cttggcaatg
    14941 atctcctgaa tcaacgctga gaccgcctgc atggcctttc cgttattgac cgcggacagc
    15001 ttttccagat cgaccttgga gagcaaagta cggtgtacgt ccctcttcag ttgttgaaaa
    15061 tctagagttt ccatattcga ttacacccgc cggggccgcc gctattcaaa caagccccat
    15121 ctttttttct tgccttgctc aaccggcttg ccgcacgcct gccgcgccat ctggcgcagc
    15181 gccttggcga tcgaggaatt ctccagcgcc agcggcgctc cggtattcag ggaccggcag
    15241 acgctgaagt agtcgttcgg cactttccac ttcaacggca ggggcagaac cttcgccatg
    15301 cggctttcct ggatctccac cttgcgcgga tcgaagcggt tcagaaccat ttcaatccgc
    15361 cgttccggca tccccggctg ctgaaaatgc gcgatgagcc gctgcgcatt ccgcaaagcc
    15421 gggatttccg cctgcgtcac caaataaatc gaatccgcca tctcgcaggc gacttgcagg
    15481 ccttcggtga aaatggaacc gccgtccacc acgacaaacg ggaaccggct gcgcaggacg
    15541 tgcagcagct tccggaggct cccgtccatc accagggttt gggcgctgca ttgatcgggc
    15601 gaggcgagca ccgcgacgcc cgatctgtgc tcgaccagca atgacgagac gaagtcctga
    15661 tcgagccgct cggagttgtg aagcgcgtcg gcgatcgtga aatgcggcgt caggcccagc
    15721 actacggcca cgtcgccgag ctgcgttttc aaatcgatga gagccacctc agcgccggct
    15781 tcctgcttga gggcgatcgc gaaattgctg gccagcgtgg tcacgcccgc tccgcctttg
    15841 gcgccgcaga agaccaggac ccggccgctg agcttctttt gcttttcgat ctccagccgc
    15901 cgcgccgtcg cgcgaatcac cgcctcggcc aatgtgtccg ctgaaatcgg ctcgctcaaa
    15961 aattcccgcg ccccggcgcg catgcagcgc accagcactt ccgggtcccg cgtggcggaa
    16021 tagaccatca ccgtggctgc gagattccgg ctgcaaatgt tctccacgag gtccagagcc
    16081 gcctcgggat caggatcgag gtccacgatc accacatcgc atccggactc gcccagtttc
    16141 gccaactggt gataattcgg atacgcgccg tattcgccgg taagcgtcac cggatgccgc
    16201 tcgagcgcgg cggccaacgc ccgccggcgc ttctcgtccg gaccgatcag ggcgaccgaa
    16261 agcatgttct taaccacggg tggatgttcg tactcgaaag tcgagttcac cgttctgttc
    16321 caccctggga cgcggccggt cccacttcca cttgagaaac tttctccccg cgcacaacct
    16381 cgatgaccgc cttcatgatc accggcggct cgggcggcgc aggggggagc gcagctatct
    16441 gcggctccgg gcgcggcgcg cgcttcaccg gtttcggggc gggagcgggc ttcggggcag
    16501 gaccgccgta gagagcctgg cgtcccacga ccgcttcctt tgccaccttg ctggtgtcgg
    16561 tcgggttccg caggaccaac tggatgtggc cctcgttggc cgccagagtc actttcgccg
    16621 cgtcttccgg attgcacagc agcgtcacga cgttcacctt ttccggccgg ccctggtcat
    16681 ccggctggat tttttgtccc gccgccagca cctgcacgtt ctccagaatc gtcttcgtca
    16741 gagattcgtc gccgccgccg gcgcgcccat tcgcgggatt gcctgtgagc agcacatcga
    16801 ccctcgttcc cggccccaca aagccggcca cagccacaac gtcatccacg cgaatcgata
    16861 cagcccgcat tcccgcagga atcaccgcgg gcaggcccgc tccggaaccc tccggcgcca
    16921 gcttcgcctg aagcagaacc tcgccgtcga aaaccgggta aagaaccgct cgtcccacga
    16981 tgtcggaagc tttcgaaaag gccccggtgg gactcgaacc gggccagtcc accaccttca
    17041 gatccccttc gctgagcttc tgcccgattt ccaaatcgcg cgccgccaca atcacccggc
    17101 tgcccgaaga cgcgcgcgcc gtgctctgcc gcagtttctg aaaaacaaaa aggcttgccg
    17161 ccccagccac caataaagcc accaaaaacg tggccaaaag ccgtgatcga ttcatcggat
    17221 ctcatctcct ctccctgcct gttacagggc gtaaaacgaa aacatcgttc ctatcgcgat
    17281 cgccgccgcg tagggaactt tgagcgcagc cgcgttttcc agccgcaact ccttgtgggg
    17341 acggaaacca tctcgaaaaa atccgctcac cagctcgccg gttccgtcga tgcatttccc
    17401 cagcgagcga ttccatagcg cgtacgccag ggccaaaaat ccgccggcca tggccgtcgc
    17461 caccagcgct gtgatcaatt gcgatgggcc gatccacgcc ccgaccgcgg cgaacaactt
    17521 caaatcgccc atgcccatcc cgcgcaggta ccacagaacg cccagcacag cagccgcgat
    17581 gccaattcca gccgcgcttt gcgcgacgcc ccgcatccct tgcgccacgc cgttcacggt
    17641 cacgccggcc gccaaaaacg gaaaaaccag ccggttggga atgcgccgcg tgcgaatgtc
    17701 gctgatcgta gctatgacca agacaatcaa cgtgggccac aaagcgatgg agtgcaccgg
    17761 atgcatcgag ccgcgttcct atggaagcgc tataggtact gcaatcccct ggccgatcca
    17821 caaaccgcct gcgcctgaat tgcttacgcg ctcccgccgg ctccgggccc gcgtctctgt
    17881 aaacgcgcgt atacgcaaaa agtcgtctgc cgtatacgcg aagagcgccc ggccggccgg
    17941 aaaacgcgaa tttcccgccg ctccgggcgc cggcacaggc cgcagagaaa agcgctgctc
    18001 gcaaaaacgt ccccgccaac gccgtgcggc agtctcggcc ggcgcgctcg gcccggcctc
    18061 ggcgccgcag cgcgcgaatc catgaaggaa catgaaggaa agcttggcgg ccgtgtcaca
    18121 tggacctggt acgggcggca tccgcggccg gagccgtctt tcacttgtga atcaaaagta
    18181 aacgatcgga aaaaatacaa gggcagaaac tcgtaggcgg gagacagagg agaacagtgc
    18241 ggcgggacga ggccgcaatg cggagaaggc gctgcatgga tggaagcgga gcggcggcct
    18301 ccgccgcccg tgctcccatc catgaaagca gttagctggt gtaagtggtg agcttggtcc
    18361 cgatgttggt gaaggcggtg ttgatgtcgg tcgccacagt gttcatgccc gccgtggcgg
    18421 cgaaggcgat cagggccacc accagcgcgt actcgatcag atcctggccg ctttcgttct
    18481 gcaaagcgac gtacagcttc aaaagaaggt tcttcattca attgttctcc tgtttcgtag
    18541 atctcgcgtt cccgcttcgc ggcaagcccg cagtgaagga acgcgctgcg aagtgcaatt
    18601 ccacgcccaa tccgccggaa cccgtcttcc ctgtttgctt tgaatgtctt accgcgaggc
    18661 tctggtttta agaaaaaaac attgtgaacg cgcgtataca gcttcagcgc gcgccgtcat
    18721 gggcggtata cggctgcgcc gcgagggaga gggtggcggg gaatagcagc gcgagatccg
    18781 cagtcggacc taccagcaaa tcgcggccgc cggcgcggac ggcctgcaag cttcccgggg
    18841 gaagccgccg gcgaagatcc tcaagatatt gcgaatactc ggggccggcg ggcagcggat
    18901 aaaaaacgaa atgaggaagc gccgccaggg cgagctcgct ctcggcccgc gtttcccggt
    18961 cctgcacggc gaacacgcgt ttgccggctg cgaactccac ctcgggaaat tggaccgtca
    19021 aatactcggg cagataatag gcggcgtggc gatacccgag gaagtgggag tcgaaaccga
    19081 tgatgaccgt atcccggggt gaacacgccg cgcggatact ccggtttaca ccaaccaact
    19141 ccgcctcgaa gtccttgagg gatttatagg aacagtatac gggcgcgtac aggaacactg
    19201 ccgtgttggc cgccgccaca gccgctgtca acgcgagttt acggcggcgc ggggcattcc
    19261 aggcggcata gagccgcgcc gcccaccgcc cgagccagac gaaaacgggc ggggaaagca
    19321 cgagcaggta tccgctgttg acgaagcgaa gaaagacgaa agtaaagaac agcagtcccg
    19381 gagcgatcca aatccacgta aattgttttt tctctctcgc ctcgcccgcg ttccgcgccg
    19441 ccagcggcag ccatgcagcg gcgcccgcca gcaaactgta aatgccgagg atggtgcaga
    19501 accgggccac agaaagggcc agcggcgagc gaaggaaagt gtgtttggct aatacaacgt
    19561 gccaaagatc ttgcagggga gcgagatagg cggcgatgcc gccggccgcc cggaccatgg
    19621 ggatgaacca ggccagtaag gccgccagca gcgcggccag gcccgctatc acttgattcc
    19681 gccgggcctt gcgcagcgaa gccagccaca gcggcgccag caggagcagg gacgattggc
    19741 ggaagcccgc agccacgccc agcgcgagcg cgctgggcac gaggaaggcg gtcctcccgg
    19801 aatagctttg ccagcaaagg tagccgatca aggcggaaaa gaaagcctcg acgatatacg
    19861 tcagggccac cgtgccgtga aaccaactca gaggcgagaa caggaagagc actcccgcga
    19921 gaacggccgc ttccccgcca aaccaagcgt ccgcaagcgc gtagatcaag gccgccgcgc
    19981 cgcagctcgc cagaacgctg accgcgacca gggccgaatt ggcgtcggca aacagcagat
    20041 tcgccagtcg cccgaggcag acatacagga aatagcccgg cggatgcggc tggtacacgg
    20101 cgggatcgaa acgccccagg gccagggcga aatttacaga gtctatgtcg tacaaataat
    20161 ggctgcgaaa aagcaggcgg ctgatcgcga cccccagcag aatcgccaag gattcgacgg
    20221 ttcgtcgaga gagcacgcac ctatttgagc aatcgagcgg ccaatacctg gccgtggaat
    20281 tgcactcata ggttagcggc cgattacacg catactgccg tatacatgcc gagacgattg
    20341 gctcaaattg gcgcgtggct gctggcagct ttgctggccg gcctcctgct tttgccgctg
    20401 cgaagccagc cgccgtggga ctttctccag ttttacgtgg cgggcaaact ggccgcgagc
    20461 gggcgggtcg ccgagattta tcacaagccg gcttatccgc cgctggtgga cgagattcgt
    20521 tcttcggaaa gccggtccga aatttcgagc gcctatttta accgcacgcc ttatttcaac
    20581 cgtccggcat tcgcggctta tctgtgcgcc ccgttcgccc ggctgccctt tcaaaccgcc
    20641 gcccacctgt ggatcctagc caatttcgcg cttttggcgg cgctcgtctg gaagcttccg
    20701 gcgtggcttc ccgccggagc gccgcttcgt ccgtggctgg cgggctttct gccgtggatc
    20761 tggtcgatcg gactgggcca ggacacgctg ttgatttcct tgctggcggc ctactcgctt
    20821 cacctggcgc tgcaaaagcg ggagatcccg gcgggactcg tgctggcagg ctgcgccttc
    20881 aagccccatc tgctggcggc gatgccgttc gctctgctcg cggcgagaaa acgaaagatg
    20941 gcggctgcct ttcttgcggc cggagcggcc ctggccgcta tctccgccgc cgttgccggt
    21001 tcaaccggct tccgagagtg gatggaactg ctccattccc ggacgaccga ctcgatgccg
    21061 gagacaatgg gcaatatccg ggcgctcgct ttgcactttg gttttgcgag cggtctggcg
    21121 gcaggcgccg tcgccgccgg ttgttttctc ttcgtactcc ggcaaggacg tttgcggcag
    21181 agttttgcgg cggcgattct cgccgccttg ctgctgggcc cgcatacata tcttcaggac
    21241 tactcgcttt tggccgtggt tgcgctgagc gctttcgatc gatgggcgca gtatgcgctc
    21301 ctgctgccct ggccgtattt ttaccgccgc gaggacatgc tgccgctgac gttggttgca
    21361 ctggccggcc tgatcgttct cgcagcgcgg gcggacacag cgcttgcacc ggatacagcg
    21421 caaacatgcg cgggggaagg gggggccgat gctcgaaaca gcgcttgcgc cgcagtctga
    21481 ggcgggctgc cgcctggacg ccttctccgg aaaagcgctg ctggggatag cgtggctggc
    21541 ggctgtgtgg atctttcttg tttcccttgc ccccgcgttc tccctgatga atccatcgca
    21601 ctcggggtat ttcccgcctg actacatgca gttttacatg gtaggccgga tggcggccgc
    21661 cggagacata gcaaagatct atgacaaggc ggcatatgaa cccctcgtcc gcgaattgcg
    21721 ctctcaggga gagcggatcg gagactaccc gtttaacagg ccgagcttcg aggcgttcgc
    21781 ctgtattccg ctttcgtggt tctcgtaccg cacggccttg ctcttcgcat ttgcggggaa
    21841 tctcgccgtc ttcggcctgc tgctctggaa gctgccgatt tggtttggcg cgccggcttg
    21901 gacccggccc tgcctgctgg tctttacgcc tttcctgtat aacattgcgc tgggccagga
    21961 tgtgctgccg ctggctcttc tcgtcgcctc caccttgagg caggcgctgc tcggaagaga
    22021 tcgtctcgcg ggcatcctgc tagggctgtg ttgtttcaag ccccatttgg tttggctcct
    22081 gccgatggcg ccgctggtgg caggcaggcg gcgaatgttt tggtcctttg ccgccacggc
    22141 aggagctttg gccgcgcttt ccatcgcggc gctgggaacc gccgggctcc gggactggat
    22201 tctcctgctt cgatctccgt tttcggacta catgccggaa acgatgtgca attggagggg
    22261 gctggctcgg catctgggcc ccgccccggc ggcggccgcg gcaattttca ttctcggttc
    22321 ggtggtgatt atcctctgcc gagcctcgga cgctgagaag gtcttggcgg cgcttgtggc
    22381 aagcctgctt ctcagtccac acacctatca ttacgactat gccttgctcg ccgtggccgc
    22441 catggcaaca ccattgccag ccgtacggat ccttctcttg ctgccctgga gttattttca
    22501 cgagctgacc aacccgctac cctggattgc cctggcgatt gtttggctcg gttccaccgc
    22561 tttgcccctt ctgcgccgcc gtgtagttgg agaaatgaat cgaacagaat cgctcgcacc
    22621 ggcaggattt taaaccctat ggcggcagtc aatccattcc cgattgcaag gcgcgcgcag
    22681 ggcggcatgg cgggaactct cgcccgatac gcgctggccg cattcgccgc gggcgcgttg
    22741 ctcgccggcg cccttcgctg caaaacagga gcgcagccgc tgctcgcctc gcaactcgcc
    22801 ggcgcattcc tgatctatgc agtgaaacgg ccgggcgctc gaagcctgtt gcgagcggcg
    22861 gcgggcgccc tggctttcgc tgtcggttat gccttgctgc gcggcaagtg gccgggcgct
    22921 tttgtctact ggccgctggt tatgggcgcg ttcctggggc tgggaggctt gctggcgctg
    22981 ctggtcgaac tgttctggtc cgcgccgggc tcggcggcga ggcgtgaggt tgcgaacagc
    23041 ctgttcgcgg tcggggtgct gcctctcatg ctgatcgcga cattcctggc gcatggcgtc
    23101 acgcgccacc tgcacccgat gacctacgac cgttttctct acgcgtttga cggagccctg
    23161 ggctgtcagc cgggctactc gctggggcgc ctttttgccg cttcgccgcc gctggccgtg
    23221 gtcagcaacc tggtctacgt cacgctgccg ctgccggctg cgctggtgta catctcgcac
    23281 ctaaaggcgc gggaaggctc gccggaactc gtcctccggg tgtttctggg cattggcgtg
    23341 gcggggtttt tcatgtacca gatctgcccc gctaccggcc cggtctacgc gttcggacat
    23401 tttccttcca atccgcccgt cttctcgctt tccgatctgg ttcctattcc gattcgcggc
    23461 ggatttctga atgccatgcc gtcgctgcac gtagcctggg cgctggcgct gctgtggaat
    23521 tgccggccgg gccaggccgg gtggcgagcc gcggccggcg tttatctctt cttcacaatt
    23581 ctggccacgc tgggaacggg agagcactat ctcgccgacc tggtggccgc ctttcccttc
    23641 acgttagcga tccaggccgc ctgcacaacc gcggcggagt tgcgatcgcg cgagagccgc
    23701 cgcgcgctct gcttcggcgg cgccgccgcg ctggcctggc ccatcgcttt gcgaacctct
    23761 ctccccagcc tgcttagtta tccgcgcctc tcctggatcc tggccggcgc cacggtgatc
    23821 gcggcggtga ttctcgcctg gccgctgctc caagcgcaga gcagcccggc gccgaaaccg
    23881 ggcgcagcgc cggagtatgc cggggcaatc ggcgcctctg aaggaaaatt cccatgttgc
    23941 tctccatcgt agtccccgtt tacaacgaag cggaaagcct gccgaggctg ctcgaagcgc
    24001 tgcgcgaggt cctgccgcag accggctgcg agcacgaact gattttcgtc aacgacggca
    24061 gccgggacgg ctccttcgaa gtgctttccc aggccgcgcg ctccgatccg cgcgtgaaga
    24121 tcctcggctt gagccggaat ttcggacatc aggccgccat caccgccgga atcgacttcg
    24181 ccgccggcga cgcggtggcc gtgatggacg ccgatttgca agatccgccc gaactcctgc
    24241 ccgacatggt ggatctgttc cggcacggct acgacgtcgt ttccgcgcag cgggtggccc
    24301 gcgattgcga cagttttttt aagcgcgcca cggcgtctct cttctatcgc ttcatgcgga
    24361 aggccatcga cagccgcctg gctccgcagg tcggcgattt ccgcctcttc agccggcggg
    24421 ccgtggccgc gctgcgcggc ttccgcgagc agcaccggtt catgcgcggg ctggtggcgt
    24481 ggctggggct caaagaagcg attttgccgt ttcaccgcca gccgcgcgcg gcgggagaaa
    24541 ccaagtactc gctatgcaag atgctccgat tcgcctggac ggccatttcc tccttttcgg
    24601 ccttgcccct gcggttgtgc ctcaccttcg ggcttctgat gacgctcggc ggattcctct
    24661 atttcgcctg ggtcctctac gtcaccgtcg ttctgaaggg gactgtgccg ggctggacct
    24721 ccctggtcgg tttgcaagtg attttttcgg gctcgacgct cgtcgccatt ggtctggtcg
    24781 gcgactatat cgcgcgaatc tacgaggaag cgaagggccg gccgctttat gttgtttccg
    24841 aagcggtaaa tttctcccac gcagaacgtc acctggagcg gatggttctt ctgcccgctc
    24901 ccgaggagcg ccatgacgca aacagcgctg aaggaacgtc ctctcagccg gccgccagcc
    24961 gaatttgatt cttacgtcga agcctacccg gagctgctgc gcgacccagt gcgggagagg
    25021 tttgcgcccg gggggcgctt cttcatagag aggaagcggc tcctgctaaa ggcgtttctt
    25081 caaggagccg gcgtagccat gcagcgagcc cgctggctgg acatcggctg cgggcggggc
    25141 gatctgctgg ccgccagcca gagcgagttc aggtatgccg ccggctgcga tttatccgcg
    25201 gggatgatcg ccggcgccaa gggcctcgat gtgcggctcc agaacgctcc ggaccagctt
    25261 ccgtttgacg acggaagttt cgatctgttg accgccgtat gcgtttacca ccatgtggat
    25321 gtcgaggtca gggcacgcct gacgacagaa gccgcccgcg ttctccggcc gggaggcatt
    25381 ttctgcgtta tggagcataa tcccttcaac ccggttaccc ggttcattgt gcggcggagt
    25441 ccggtggacg cgaatgcgga gcttctgagc gccggggaag tcaggcggct ggttcgggtg
    25501 gcggggatgc aggtgctccg cacccaatac tttcttcttt ttcccgaatt tgtatatcag
    25561 cgtctggcgt ggctggaggc cggtttgtcg ggccttccgc tgggcggcca gtatgctgtg
    25621 tttggctcga ggccttaatc ggcacgctac ggatgtctct gctggcgatt gaaacggcgc
    25681 taaccgtgat cacagccgga ctggcgtttg ccggaccgag gctgggatcc cggtggttcc
    25741 ggcgctggga agacgcgttc gcgcggctgg cccggagaaa gggtttgtct atcgtagtag
    25801 taggtttagc tgccttgctg gcgcggctgg ccctgctgcc cgcgctgccg attcccgaac
    25861 cgggcgtgca tgacgagttc agccatctgc tcgcggcaga aaccttcgcc tccggacgtc
    25921 tggccaatcc gccgcacccg atgtgggttc atttcgagag cttccatata gaccagcttc
    25981 cgacgtacat gtcgatgtat ccgccggggc agggtcttat tttggccgct gggaaatgga
    26041 ttttcggcca tccgtggttc ggagtgtgga taagcacggg agccatgtgc gcggcgttcg
    26101 gctggatgct ccagggctgg ctgccgccgg gatgggcgct gctgggcggg tttctggccg
    26161 tgctgcggct cggaattttc agctattgga tgaacagcta ctggggcggt gcggcgcccg
    26221 ccctgggcgg agcgctgatc ctcggcgcgc tgccgcgcct gttgcgctcg ccgcgcgccg
    26281 gtcatgccgc gctgctggcg ctcggcctgg cgatcctcgc caacacacgg ccctatgagg
    26341 gattggtgct gagcgtcccc gtgctgcttg cgcttctcgt ggccgcgatc atgggagagc
    26401 cgcgccgggc gcggctgctg cgcagagcgg cgcttcccgc cggcgcgatt ctgctggccg
    26461 ccgccgccgc gatgagctat tacaattggc gcgtttttgg aaatccgctc acgttgccgt
    26521 accagataaa ccgcgctaca tacgcggtcg cgccgtattt tccctggcag acgccccggc
    26581 ccgagccggc gtaccgccac cgtgtgctgc gcgattttta cgtcggctat gagttcgccg
    26641 atttcgaaaa gacgcgcacg cccgggggct ttctctccag aacgcgggaa aagctcacga
    26701 cggtgttctc cttttacttc gggcctgcgc tggcgctccc gctggcgttt ttgccgcggg
    26761 cgctgcgcga ccggcgcttg cgtcttctgg cgctggccgg cgccgcgaca gcgctgggac
    26821 ttgcagccga ggtctggctg atccctcatt acgccgcgcc ggccacatgc ctgctgtacg
    26881 ctctgctcct ccaggcgatg cggcatttgc gcgcgtggcg gccggagggc cgcttcctgg
    26941 tacgcggcct ggtcatggtc tgcgt
//
LOCUS       DASREK010000038         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2000862, whole genome shotgun sequence.
ACCESSION   DASREK010000038 DASREK010000000
VERSION     DASREK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000862"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFA54_02425"
     CDS             <1..252
                     /locus_tag="VFA54_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49687.1"
                     /translation="TGDDLETTRKAGKKYRCIRSAEAQRVESEQAHSWPFDCSPQGRR
                     QGEMGLLAVGGALMIAFVLALSLQWLLLAAILRQVTRSR"
     gene            complement(594..2708)
                     /locus_tag="VFA54_02430"
     CDS             complement(594..2708)
                     /locus_tag="VFA54_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZS49688.1"
                     /translation="MHTNQLIHESSPYLLQHAHNPVNWYPWGPEALELARRENKLIFL
                     SIGYAACHWCHVMEHESFENDEVAEILNRDFVSIKVDREERPDLDEIYMTATMLYTGG
                     QGGWPMSVWLTPDLKPVFAGTYFPLESHYGRPGFKELLRAIARAWRERPDELREQADR
                     VVGAIASMQQIEKGDVLTRERISQIVRRLAEAYDPVYGGLPSGGSNKFPPSMSMDLLL
                     REYRKSGDRLLLGTVEKTLEKIGDGGIYDHLGGGIHRYSTDVRWLVPHFEKMLYDQAL
                     VGSIYLDAYQATGRPMFAERARGIFDYVLRDLQSPEGGFYSSEDADSEGLEGKFYIWT
                     FDEVRQILGEEDAALFASHYDITESGNWEHPGDAHVPQGPKNILHVVRPLEAIARLNN
                     LDLPDLKKRFDAMRRKMLEARSKRPRPSLDDKILTGWNGLMIAALAKGAAVLDDRRYG
                     EAAERAAGFILSRLVENGRLLAAYGKGKARLKAYITDYAFLVEGLLGLHEWSGDRKWL
                     EQASRLADTAIQYYWDTQDGGFFFTASDHEQLIHRSKISTDSAIPSGNSVMLMNLQRL
                     AVLLGREDLREKAAEIIRLFAAAIEKHPFAYDRMLAGIEAWHDGFVEIAIIGPEHSPA
                     TRELWRAVYRGYLPNKVVVRSEQPDPRLPLLANRAMIGGRPTAYVCRNYSCRQPMTSA
                     EELADQLGFSRQQDAERAGTVN"
     gene            2796..3119
                     /locus_tag="VFA54_02435"
     CDS             2796..3119
                     /locus_tag="VFA54_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HZS49689.1"
                     /translation="MSCAPPPGPMRPEKPVKRKVFCVKFQKELPGLDEVPFDNELGQR
                     IYENVSRDAWDLWKEHLKMIMNEYRLNLATREAQEIVIKQMEDYFFGEGAALPPDYVP
                     PKTKG"
BASE COUNT          611 a          960 c          929 g          660 t
ORIGIN      
        1 accggcgacg atctcgaaac tactcgaaag gcgggcaaaa aataccgctg catccgatcg
       61 gccgaagcgc aacgagttga aagcgaacag gcgcactcgt ggcccttcga ttgctcgccg
      121 cagggccgga ggcagggaga aatggggttg ctcgccgttg gtggtgcatt gatgatcgcg
      181 tttgttttgg ctttgagttt acagtggctg ctgctggcgg ccatcctccg gcaggtgacc
      241 cgcagccgtt agcccatccc agatagctta ttttcgcgcg cccctctgtg gttttttgag
      301 ccgcgaccgg cggagcttgg cggctccccg cttcggcttt gaaatcgcaa aggctccgct
      361 tcccaagtag acgacttttt ttgttttgtc ttcatcagcc atttcgatct ccgttctcaa
      421 actcctgggc gggcagaccc aaaatttcga tcaacgcggc ccccgggccc ggctccttgc
      481 ccggcgtgct cttccctctg ttccgggttc ataagaattt gcgccggccg ttcggtgcga
      541 ggggcgaggg gcgaggcgaa catgccgcgg agccgccagc ctctcgcacg atcttagttt
      601 actgtaccgg cgcgctcagc gtcctgctgc cgggaaaacc ccaattgatc ggccagctct
      661 tcggcggagg tcatcggctg acggcaggaa tagttccggc aaacataggc cgtgggccgc
      721 ccgccgatca ttgcccggtt tgccagcaga ggcagcctcg gatccggctg ctcgctccgg
      781 accacaacct tgttgggcaa atagccgcga taaaccgcgc gccacagctc cctggtcgcg
      841 ggcgagtgct cggggccgat aatggcgatc tccacaaatc cgtcgtgcca ggcttcgatg
      901 ccagccagca tccgatcgta ggcaaacgga tgtttctcga tcgccgccgc gaaaaggcgg
      961 ataatctcgg ccgccttctc gcgcaagtcc tcgcggccca ggagcacagc cagccgctga
     1021 aggttcatga gcatgacgga attgcccgaa gggatcgcgc tgtcggttga aatcttactg
     1081 cgatgaatga gctgttcgtg gtccgaggct gtgaagaaaa acccgccatc ctgcgtgtcc
     1141 caataatatt ggatcgccgt atcggcgagg cgggaggcct gctccagcca cttcctatca
     1201 ccggaccatt cgtgcaagcc gagcagccct tccaccagga aagcgtaatc ggttatgtag
     1261 gcttttagcc gcgctttgcc cttgccgtaa gcggcgagca ggcgcccgtt ctcaaccagc
     1321 cgcgacagga tgaagcctgc cgcccgctcg gccgcttcgc cgtaacggcg gtcgtcgagc
     1381 acggccgcgc ctttggccag ggccgcaatc atgaggccat tccacccggt cagaattttg
     1441 tcatcgagag acggccgcgg ccgcttcgag cgggcttcga gcatcttgcg gcgcatggcg
     1501 tcgaagcgct ttttgagatc tggcagatcc agattgttca gcctggcgat ggcttccaaa
     1561 ggccgcacta catgcaggat attcttgggc ccctgcggga cgtgtgcgtc ccccggatgc
     1621 tcccagttgc ccgattccgt gatgtcgtag tgcgacgcga acagcgctgc gtcctcctcg
     1681 cccaggattt ggcgaacttc atcgaacgtc cagatataaa acttgccttc caggccctca
     1741 ctgtctgcat cctcgctcga atagaagccc ccttccggac tttgcaggtc ccgcagaacg
     1801 taatcaaaga tgccgcgcgc ccgctcggcg aacatcggcc ggccggtcgc ctgataagcg
     1861 tcgaggtaaa tggagccgac cagggcctgg tcgtaaagca tcttctcgaa gtgcgggacg
     1921 agccagcgga catccgtcga gtaccggtgg atgccgccgc ccaggtggtc gtagatgccg
     1981 ccgtcgccta tcttttccag ggtcttttcc acggtcccga gcaggaggcg atcacccgat
     2041 ttgcggtatt cccgaagcag caggtccatc gacatgctgg gagggaattt gttgcttccc
     2101 ccactcggca acccgccata aacaggatcg taagcctcgg ccaaacggcg cacgatttgc
     2161 gagatacgct cgcgggtgag cacgtctcct ttttcgatct gctgcatcga cgcgatcgcg
     2221 ccgacgactc gatccgcctg ctcccgcagc tcgtcgggcc gctcgcgcca tgcccgcgca
     2281 atggctcgca gcaactcctt aaacccgggg cgcccataat ggctttccag cggaaaatag
     2341 gttccggcga aaaccggttt caggtcgggc gtgagccata cgctcatcgg ccagccgcct
     2401 tggcccccgg tgtagagcat ggtcgccgtc atgtagattt cgtcgaggtc tggcctttcc
     2461 tcgcggtcta ctttgatcga gacaaagtcg cgattcagga tctccgccac ttcgtcgttc
     2521 tcgaagcttt cgtgttccat cacgtggcac cagtggcagg cggcatagcc gatgctgagg
     2581 aaaatcagct tgttttcgcg gcgcgccagt tcgagcgctt ccggtcccca ggggtaccag
     2641 ttcaccgggt tgtgggcatg ctgcaagaga taagggctgc tttcatggat caattgattg
     2701 gtatgcatgt ctgagccagt tgaggcgtgc gataatgcta ttgtcgctgg agcatccaga
     2761 gaatacaacc tgcgtttttc tgaaagggaa gaacaatgag ctgcgcaccg cccccgggac
     2821 cgatgaggcc ggagaaaccg gtcaaacgga aggtgttctg tgtaaaattt caaaaagagt
     2881 tgcccggtct ggacgaagtg ccttttgata acgagctcgg gcagcgtatc tatgagaacg
     2941 tttcccggga tgcctgggac ctttggaaag agcatctcaa gatgatcatg aacgagtacc
     3001 ggctcaatct cgctacgcgg gaagcacagg aaatcgtgat caaacaaatg gaagattact
     3061 tcttcggcga aggtgcggcg ctgccgccgg actacgttcc tccgaaaacc aaagggtgac
     3121 ctctatccag gcatcgctgc cgattcccct cccagatcgg
//
LOCUS       DASREK010000039         4683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2167206, whole genome shotgun sequence.
ACCESSION   DASREK010000039 DASREK010000000
VERSION     DASREK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167206"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VFA54_02440"
     CDS             complement(<1..819)
                     /locus_tag="VFA54_02440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZS49690.1"
                     /translation="MHNHHHRGATGKRLQLSLLATILFIVAEFFAGLRAHSLALLSDA
                     GHNFTDALSLLLAWFAFFVESKPPSETKTYGYQRAGVLAAFVNALALVVLAGFIFFES
                     YRRLGAPQPVAERLMIGVAAIGLLVNVAIMIALREAGRRDINIRSAFVHMLGDAVSSA
                     GIIVGGVLIAWTGYNWIDPALSILIGLMILWTGWDIIKESLNILLEGLPKDMQLETVM
                     EAMRSIPGVIDVHDVHIWSLASHSHAMSCHVMIEDLPPSSSETILRSVNELLASR"
     gene            1035..1709
                     /locus_tag="VFA54_02445"
     CDS             1035..1709
                     /locus_tag="VFA54_02445"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS49691.1"
                     /translation="MKRATGLLLAAGAGLWIMLGAPSNSAPNTLTPQEKAEGWILLFD
                     GKTLNGWISRGGGAEWRVVDGAIMTDSRRGGGLYTAQQFSDFILKAEFRTTDDVDAGM
                     YLRIQPPEPNRMRGTDPKAKKTGERRGYELQIRDTARKGGYNTGSIIDAIQASPAHIK
                     SNQWNTYEVTAQGDHFIIVFNGEKVAEGHDGKFSRGSIGLQWAHPERVSEGHKIEFRN
                     LKIRPL"
     gene            complement(2025..2288)
                     /locus_tag="VFA54_02450"
     CDS             complement(2025..2288)
                     /locus_tag="VFA54_02450"
                     /inference="COORDINATES: protein motif:HMM:NF018247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZS49692.1"
                     /translation="MAANIIDLNTASEQEIASIPGVGPDRAKDLIANRPYTDWDEVAE
                     VPGVSESLIEDMIENGVTLGSEADTEEEEDLDDIEEGGEEEAA"
     gene            2519..3013
                     /locus_tag="VFA54_02455"
     CDS             2519..3013
                     /locus_tag="VFA54_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49693.1"
                     /translation="MAGAEHNVFSLGIQAEKLRLFGFRDQSMRFRTLLTLGSRFVLCC
                     LLCASASVMAQKRVSSERESRPELGDKPDAPLKVETIKGTLKKIDLEKRTVTIAHGES
                     ESVFGFPTAAGREKITLSKKVAKTLGKKSLRLEEVQSGSQVKVAYYPALGTIMELTIE
                     ELGR"
     gene            complement(3122..4345)
                     /locus_tag="VFA54_02460"
     CDS             complement(3122..4345)
                     /locus_tag="VFA54_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS49694.1"
                     /translation="MYARSRMDAVAPRATPRPAIVRSAMGGVFLLGCLVALMGALFPV
                     WAYYVDFDPGTAGSYFLAFNLGVFTAAMASRGILEKLGMRRLLVTACLATSAAMFALA
                     AILSPTALVLPFIVLGFAAGMLTTGVSWMLFDAMSAPMAGVLLSLAGVFFGCGAVCFT
                     LLIWATVHTLSAPNILRLTAVLPLGLAVLYLGQKSLAQPALKVARPFRLSIRATASPA
                     AVLLSLALFFQSGNEWALGGWLAIYLIRRLGVRSETALLSLAFYWAMLTLGKLLAPRC
                     AWSESPFRLLSASAAASLFGCLLLLSTTGAEGAAAGVLFTGLGLGAAASVVLGMIGER
                     FPHYHPGFFNGLFSFSLVGGMLAPWLIGQLADAWVIQWAIGIPALGALLVYLLLCAFL
                     LEMRIAGSSRPISSS"
     gene            complement(4467..>4683)
                     /locus_tag="VFA54_02465"
     CDS             complement(4467..>4683)
                     /locus_tag="VFA54_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49695.1"
                     /translation="LEIKRLLYHEGFTISGARKALKERTRAARKKQSPGGPAQMPLIP
                     DTSQLRPKLQEMRKEIEEILTILKKPR"
BASE COUNT         1065 a         1390 c         1425 g          803 t
ORIGIN      
        1 tcgggacgca agcaactcgt tcacggagcg caggatggtt tcgctggagg acggcggcag
       61 gtcttcgatc atcacgtgac aactcatggc gtggctgtga gaggccaggc tccagatatg
      121 cacgtcgtgt acgtcgatta ctcccggaat gctgcgcatc gcctccatga cggtttcgag
      181 ctgcatatcc ttgggcaagc cttccagcag aatattcagc gattccttga tgatgtccca
      241 gcctgtccac agaatcatca ggccgatcag aatggaaagc gcggggtcga tccagttgta
      301 tccggtccac gcgatcagga ccccgcctac gatgatgcct gcggaggaaa ccgcatcccc
      361 gagcatgtgc acgaaggcgc ttcggatatt gatatcgcgg cggccggcct cgcgcagcgc
      421 tatcatgatc gcaacgttca ccagcaggcc gatcgccgcc acgcctatca tcaaacgctc
      481 cgccaccggc tgcggagcgc cgagccgccg gtagctctcg aaaaaaataa agcccgccag
      541 caccaccaga gcgagcgcat ttacgaaggc cgcgagcacg cccgcccttt gataaccgta
      601 ggtcttggtt tcgctgggag gtttggattc cacaaagaag gcaaaccagg cgagcagcag
      661 ggaaagcgca tcggtgaaat tgtggcccgc gtcggaaagg agagccagcg aatgagcccg
      721 gagaccggcg aaaaactccg cgacgatgaa tagaatcgtc gctaacagcg aaagttgcag
      781 ccgcttgccg gtggcgccgc gatggtggtg gttgtgcacc acacctcctg cccacagttt
      841 aatacaacgg gaggaagcga gagaccgagc gcgcgggctg gcgtgacgcc ggcaacggcg
      901 ctctaagaga gccgcgaacc cattgggggc tcgaggtgca agcagccacg ctcgattcca
      961 tccaccacga aaaaactttg ctggccgatc cggccgaaag cggagataat ggtaccccga
     1021 agggaggagg gcacatgaag cgagcaacgg gactcctgtt ggcggccggc gccggtcttt
     1081 ggatcatgct gggggctcca tccaattctg cgccaaatac cctgaccccg caggaaaaag
     1141 cggagggctg gattcttcta ttcgatggca aaactctcaa tgggtggata tcgcgcggag
     1201 gcggagccga gtggcgcgtt gtagacggcg cgataatgac cgactcgcgc aggggaggag
     1261 gtctttacac ggcgcagcaa ttcagcgatt tcattttgaa ggccgagttc cgaacgaccg
     1321 acgacgtgga cgccggcatg tacttgcgaa tccagcctcc cgagccgaac cgcatgcgcg
     1381 gaaccgatcc caaagcgaag aaaaccggcg agcggcgcgg ctatgaacta caaatccgcg
     1441 acacggcgcg caaaggcggc tacaatacgg gaagcatcat cgatgccatt caagcttcgc
     1501 cggcccacat aaaatccaac cagtggaata cctacgaagt cacagcccag ggtgatcatt
     1561 tcatcatcgt cttcaacggc gagaaggttg ccgagggtca tgatgggaaa ttctcgcgag
     1621 gatccatcgg cttgcaatgg gcacatcccg agagagtttc cgagggacac aaaatcgaat
     1681 tccggaattt gaagatcagg cctctgtagc ggaaaaactc aaaccgtccc agacagacgt
     1741 cgtgttccgg cggcctccgc atgcgagaag ggaagcagct aagtccggaa cacgggcccc
     1801 agcctccggc tggccgtgtc aaggggcgct gctgcccgaa accgcgagcg tgaggagcgg
     1861 ggcgccgaaa aacgcgtttt tcaagaagtc tggaatccga ccctcgccag gaacactcag
     1921 tacctttgga gcgagcccct gccaccaacc aggctcggct gagaattagg cagctttaca
     1981 acagccggct accgggcggt cccgttgaca tttgaggacg gcagctaggc ggcctcttcc
     2041 tctcctcctt cttcgatatc gtctaagtct tcttcctctt cggtatcggc ctccgatccc
     2101 aaggttactc cgttctcaat catgtcctct atcaagcttt ccgacactcc aggcacctcc
     2161 gcgacctcat cccagtccgt atagggccga ttcgctatca gatctttcgc ccggtcggga
     2221 ccgacgcctg gaatgctcgc aatttcctgc tcgctggctg tgttgagatc gataatattt
     2281 gctgccataa cttcctcctg gctcgtttgt gacctcctca ctgcacgggc gctgccaacg
     2341 tgctgagccg ccctgagagg tttctgaccc aggttgggat tgttaccgag gcccgtggcc
     2401 gccgtgaggc gcagggcgcc ggttggacca gcagggtaag cccgcggctg gagataacgc
     2461 gcatccggag gaccggagtt tgggccgccc gacgcgctgc catccagtct tgacgccgat
     2521 ggcgggcgcc gagcacaatg tgtttagtct gggaatccag gcggagaagc tacgcctctt
     2581 cggctttagg gatcagagca tgcgattccg tactttattg acgcttggta gccgttttgt
     2641 cctgtgctgt ctgttgtgcg cttctgcttc ggtgatggcg cagaagcggg tgagcagcga
     2701 gcgggaatca cgtccggagc tcggcgataa gcctgacgcc cctttgaaag tcgaaacaat
     2761 caaggggact ctgaaaaaga tcgatttgga gaagcgtact gtcaccatcg cgcacggtga
     2821 atccgagagc gtcttcggat tccccacggc ggccgggcgg gagaaaatta cgctgtccaa
     2881 aaaagtagcg aaaacactcg gcaaaaaatc cttgcggctc gaagaagttc agagcgggag
     2941 ccaggtcaag gtggcttatt atcccgcgct cggcaccatc atggagttaa cgatcgaaga
     3001 actgggccgg tgaagggctg ccaatctccg acgaattctc gttccgcgcg ccgcgaaaat
     3061 tcggctagcc cgacggcgca tcacaacgcc gttgaacttg gcggcgcgca gtcgcttttt
     3121 ctcagctcga ggaaattggc cgggagcttc cggctatgcg catctcgagc aggaaggcgc
     3181 atagcagcaa gtagaccagc agcgctccca aagcggggat gccgatggcc cactggatga
     3241 cccaggcgtc ggccaactgg ccgatcagcc agggagccag catgcctcct acgagggaaa
     3301 acgagaacag gccgttgaaa aaccctgggt gataatgcgg gaatcgctct ccgatcatgc
     3361 caaggactac cgaggctgcg gctccgagac cgaggccggt gaaaagtacg ccggccgcag
     3421 cgccctccgc tcccgtggta gaaagaagca atagacaccc gaacagcgat gcggccgcgc
     3481 tggccgatag caggcgaaac gggctttccg accacgcgca tcgcggggcg agcagcttcc
     3541 ccaacgtgag cattgcccag tagaacgcca ggctgaggag agcggtctcg gacctcacgc
     3601 ccaggcggcg aatcagatag atagccagcc atccaccgag ggcccactcg ttgccagatt
     3661 gaaaaaacag cgccagggaa agcaaaacgg ccgccgggga ggcggtggcg cggatcgaaa
     3721 ggcgaaacgg ccgcgccact ttcagcgccg gctgcgccag agatttttga ccgagataca
     3781 gaaccgccag gcccagaggc agcactgccg tcaaccgcag aatgttcggg gcgctcagtg
     3841 tgtgaacagt ggcccagatc aggagcgtaa agcagacggc gccgcagcca aaaaatacgc
     3901 ccgccaaact gagcagcact cccgccatgg gggcgctcat agcgtcaaac agcatccagg
     3961 aaacgccggt ggtcaacatg ccggcggcaa aaccgagcac aatgaaaggt aaaaccagag
     4021 ccgtggggga gaggatcgcc gcgagcgcga acatcgcagc ggaagtcgcc agacacgccg
     4081 tgaccagcaa acgccgcatc ccgagctttt ccaggatgcc gcgcgaagcc atcgcggcgg
     4141 tgaacacgcc gagattgaac gccagaaaat agcttcccgc ggtgccagga tcgaagtcca
     4201 catagtacgc ccagaccggg aatagggcgc ccatcagggc gacaaggcag cccagcagaa
     4261 aaactccgcc catcgccgaa cggacgatcg ccggccgggg cgtggcccgt ggagcaacag
     4321 cgtccatgcg tgatctggca tacattctac agagaaatgc cctgccgcgg ccagcgattt
     4381 tcgcgtgcgg catcaggacc gagaaagggc tccttgcttg cggagtattt tttccagggc
     4441 agatggcggc ccggggacgt ccggcgctag cggggcttct tcaaaatggt aaggatctcc
     4501 tcgatctcct tccgcatttc ctgaagcttc gggcgaagct gagatgtatc gggaatcagc
     4561 ggcatctggg ccggcccgcc gggcgactgc ttcttgcgag cagcgcgggt tctctctttg
     4621 agggccttcc gggcgccgga aatcgtaaag ccttcgtgat acaacagccg cttgatctcc
     4681 agc
//
LOCUS       DASREK010000040         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_848370, whole genome shotgun sequence.
ACCESSION   DASREK010000040 DASREK010000000
VERSION     DASREK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848370"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFA54_02470"
     CDS             complement(<1..194)
                     /locus_tag="VFA54_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49696.1"
                     /translation="MTTHLPASQTPSNRLFSMLQPLGGLRCLAVLVAAVALAALGGCA
                     NTPPEGSARNLGTNTSWSSN"
     gene            458..1798
                     /locus_tag="VFA54_02475"
     CDS             458..1798
                     /locus_tag="VFA54_02475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
                     /protein_id="HZS49697.1"
                     /translation="MLLGALALSSQAVRAAPEAQEGPASRPARPARPGAQAARPGALL
                     DPAIILKRWRTVADGLGLSDDQKKKVDSILDDADKNAADMAGEIKDLPPRERIQKVGP
                     FLRGVHDQIVELLDDGQKEQFQQKTAPGGPLMALRQNLQKLDLSDEQKQKVRDLMMEM
                     QGKARDLRESAGNPDEMREKMQGLQEELRDRLKEILTPEQQQKLRDLMGEQGNNEGRP
                     AKRGAKAGAAAGADAGENMMKPGEMMKPDEATAEDHHAPAPKPAAEVEDALPAQTLHL
                     GDAAPEFRLTGLDGKTFELSNMSGTPVVLEFGSYSCPVFRSHAAAMEKLRTQYSGRVR
                     FLIVYTKEAHPVGGWEVERNKQEGIKVQQPTDLTGRKKLAEEAREKLNLATPILLDDM
                     NDSTTTAYGGFPNGAVVIKDGQVVASEKWVDPPALKAAVDKALAARPTTRPAQE"
     gene            2062..>2277
                     /locus_tag="VFA54_02480"
     CDS             2062..>2277
                     /locus_tag="VFA54_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49698.1"
                     /translation="MSGKQTNLTKRNDRKISARAVVGSLAGKLLNPGQLSQAARLQLR
                     HKVHRHSLEDAQLALYSRILPSDFLHLG"
BASE COUNT          467 a          675 c          766 g          369 t
ORIGIN      
        1 aagttcgagg accaggaggt gttggtgccg aggtttcggg cgctgccttc gggcggggtg
       61 ttggcacatc cgcccagcgc ggccagcgcc acggcggcaa caagcaccgc gaggcatcga
      121 aggccgccga ggggctgaag cattgaaaac aagcgattgg atggggtttg gctcgccgga
      181 aggtgcgtgg tcacggaggt tctcctacga ggcgctgggt ccgttgccgc catggcgcag
      241 acccggtggg atttcggttc gcaaggatag caacgcgacc cgtttaccgc aaactacgct
      301 ttgccgcggc acagccctgt ccgggggtcg ggtaaaataa cccgacgcag cggacgttct
      361 atcttcaaag ccggcgaatc gagccggaca cgtcctctac ggcagggaga ttcaaatgct
      421 cagcgttcga cccggcgtca cctttgcgat ttgttttttg cttctgggcg cgctggccct
      481 ttcgtcccaa gcggtgcggg cggcgccgga ggcgcaagag ggccccgcca gccggcccgc
      541 gcgccccgcg cggccgggcg cccaagcggc gcggcccggc gcgttgctcg atccggcgat
      601 catcctcaag cggtggcgga cggtggccga cgggctgggg ctcagtgacg atcagaagaa
      661 gaaagtggac tcgattctcg acgacgccga caagaacgcc gccgacatgg ccggcgagat
      721 caaggatttg ccgccgcggg agcggatcca aaaggtcggc ccgttccttc ggggcgtgca
      781 cgaccagatc gtcgaactgc tggatgatgg acagaaggaa cagttccagc agaagacggc
      841 gcccggcggc cccctgatgg cgctgcggca gaatctgcag aaactggatc tcagcgacga
      901 gcagaagcag aaggtgcgcg acttgatgat ggagatgcag ggcaaggcgc gcgacctgcg
      961 cgaatccgcc ggcaatccgg acgagatgcg cgagaagatg caggggctgc aggaggaact
     1021 gcgcgaccgg ctgaaggaaa tcctcacgcc cgagcagcag cagaagctgc gcgacctgat
     1081 gggcgagcaa gggaacaatg agggcaggcc cgccaagcgc ggcgccaaag ccggcgcggc
     1141 cgcgggcgcc gacgcgggcg agaacatgat gaagcccggc gagatgatga agccggacga
     1201 ggcgacggcg gaggatcatc acgcgccagc gcccaagccc gccgccgaag tggaagatgc
     1261 gctgccggcg cagacgcttc accttggcga tgcggcgccg gaatttcgcc tcaccgggct
     1321 ggatggcaaa acgttcgagc tttcgaacat gagcggaacg ccggtggttc tggaattcgg
     1381 ctcgtattcc tgcccggtct tccgcagcca cgccgccgcc atggaaaagc tccgcaccca
     1441 gtattccggc cgggttcgct ttctcatcgt ttacacgaag gaagcgcatc ccgtcggcgg
     1501 gtgggaagtc gagcgcaaca agcaagaagg cattaaggtc cagcagccga cggatctgac
     1561 gggccgaaag aagctggccg aggaggccag ggaaaagctc aatctggcga cgccgattct
     1621 tctggacgac atgaacgatt caacgaccac cgcctatggt ggattcccga atggcgcggt
     1681 ggtaatcaaa gacgggcaag tggtcgcgag cgagaagtgg gtagacccgc cggcgttaaa
     1741 ggcggcggtg gataaagcgc tagccgcccg gccgaccacg aggccggcgc aagagtgaac
     1801 agcgctaatc gatcgtcaat tcgagctcaa attgtctcgg cctgggaaga gctgtggggt
     1861 tctgctcgcg ctcggctctc gttctcctcg gcgctctctc cttcattagc gggtgtactt
     1921 gcaaggatgt ttcgaaggta tgcagagttt cgccattgag cgaattgccc ctttgtgaca
     1981 caactgttgg gcgatcccaa gccgttttgt gatcggcaac gggaacgctt tggcatttcg
     2041 atgctttcaa caagagaatt catgagtggc aaacagacca acctcacaaa gcgcaacgac
     2101 cgcaagatca gcgcccgcgc cgtcgtgggc agcctggccg gaaagctgct gaatccgggc
     2161 caactctcgc aggcggcgcg cctccagctc cgtcacaagg tgcatcgtca ttccctggaa
     2221 gatgcgcagc ttgcgctcta ctcccgtatt cttccgagcg actttctgca cctgggc
//
LOCUS       DASREK010000041         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1506006, whole genome shotgun sequence.
ACCESSION   DASREK010000041 DASREK010000000
VERSION     DASREK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506006"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..1680
                     /locus_tag="VFA54_02485"
     CDS             415..1680
                     /locus_tag="VFA54_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS49699.1"
                     /translation="MPAKVRVSAFCMLAFALASLHAEERLFGPRELADELMKSNPEIQ
                     AARHRLEAAVKRPPQLGALPEPAISYTNFGVGHPFSGLQRSAFAYQGFGVSQEFPFPG
                     KRALASEQARREAEGEQENYRSVVLEVSARLKIAYFEWLAAIKAIDLTRKNRDLLGRY
                     EEIARNRYTVGKGLQQDVLKAQLELASLEPQLAMLEEKRERREAEISSLLALPGVKLR
                     APAEWEPSRFSASLQELVKTAEDSPRLRAGQKVVDARMVGVRRSRKDFRPDFRVNLQW
                     QHTGAAFPDYYMAAVEVKIPLYYGRNQRYALEESYARLQEAKQNYEAARQSAIYELKE
                     QYLAIQSSERILSLYRTALIPQASLAVESATSAYEVGNLDFLSLLANLTNLIELQLQY
                     YYEVERHEEAIARLEPIAGRELIPFGRTN"
     gene            1680..>3270
                     /locus_tag="VFA54_02490"
     CDS             1680..>3270
                     /locus_tag="VFA54_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS49700.1"
                     /translation="MSKQRIRTLAVGVLVAAAAGALVYFGAGRRRPVHRNAAGTAAAR
                     NIYYCPMHPAYRSDKPGNCPICSMKMVKLEEPAQPASVPVPGTTAPASSSASEAAQPG
                     ISQSGAIFVPPEKQQLIGMRSVRAEIGTLTKEIRTVGKVSYDETRLTHIHSKVSGYIE
                     DVFADYVGKPVRAGDPLFTIYSPDLVATEQEYLLALRSRNLLRTSTLPSVAEGSENLV
                     TAARERLRLWDVTESEIRSLETEGKVQRAITVYSPVAGVVIERAAYHHGTFVDPSKDL
                     FTIVDLSRVWVLGEIYETDLPFVHPGEIAEIELPYSGRKIRGPIDFIYPFLDPKTRTT
                     QVRIQFPNPNLTLKPDMFTNIRIVVPLGRQVLVPVDAVMDTGTEQYVFIDEGDGYVQP
                     RQVKIGAEAGDKVGVQQGLRPGERVVTSANFIIDSESRLQGAFANMGRPSGPFASAAS
                     GGGQAVAVEVLEPKLAKTGRNPIRLRINDGAGNPVAGAQVEVTLFMPQMGAMPPMTSR
                     AALADSGNGVYSGQIEFPMACT"
BASE COUNT          648 a         1013 c          989 g          620 t
ORIGIN      
        1 tacactagcg ccgtgctgct catggcaacg ttgctgcttg ccaagccctt cgactgcttt
       61 agcagtacgg gcctgactcg ggaagcggca ggctgttgtg ctaaaggaaa atgcctgcct
      121 acgaggaatt ctgacgactg ctgccggaaa accgcgccgg atgccaaaca gttcgttggc
      181 gcggcgagcg ccactatgtg tcattccttg gccgtttcag gtccaccggt ttgcagtgcg
      241 ccggctccgc ccatcgcccg gttggcctcc gctcctttga gcaccgccca ttcacccccg
      301 cattcggcgc ccggctccaa cctcgctatc cctctcctga tctagcgcgc agcccggcgt
      361 gtttccgcgc gccggcgtgc ttttctcttt tctaaacacg cttcaggagg ttgtatgccc
      421 gcgaaagtgc gcgtgagcgc cttctgcatg cttgcattcg cgctggcgag cctgcatgcg
      481 gaggagaggt tgttcggccc gcgcgagctg gccgacgagt tgatgaagag caatccggaa
      541 attcaagcgg cccgccaccg tctggaggct gccgtaaagc ggccgccgca actgggagct
      601 ctcccggagc cggcgatcag ctataccaac tttggcgttg gccatccctt ctccggattg
      661 cagcggagcg cctttgccta ccagggattc ggagtgtcac aggaattccc cttccccgga
      721 aaacgcgccc tagcctccga gcaggcccgg cgcgaagccg aaggcgagca ggagaattac
      781 cgctcggttg tgctcgaggt tagcgctcgc ctgaagatcg cttacttcga atggctggcg
      841 gcgataaagg ccatcgatct gacacgcaaa aatcgcgacc tgctcggccg ttacgaggaa
      901 atcgcccgca accgatacac ggtaggcaag ggactacagc aagacgtgct gaaagcccag
      961 cttgaacttg caagcctcga gccgcagctt gccatgttgg aagaaaagcg agagcggagg
     1021 gaagcggaaa tcagttcgct gcttgctttg cccggcgtga agctgcgcgc gccagccgaa
     1081 tgggagccga gccggttttc ggcatctctc caggagttgg tgaagacagc ggaggactcc
     1141 ccgcgcttgc gcgccgggca gaaagtggtg gacgcccgca tggtcggcgt tcgccggagc
     1201 cgcaaagact tccggcccga tttccgcgtg aacctccagt ggcagcatac tggagcggcc
     1261 tttccggact actacatggc cgcggtcgag gtgaagatcc cgctctatta cggccggaac
     1321 cagcgctatg cgctcgagga gtcgtatgcg cgccttcaag aggcgaagca aaactatgaa
     1381 gccgcccgcc aaagcgccat atacgagcta aaagagcaat atttggccat tcagagcagc
     1441 gagcgcattc tgagcctgta caggaccgcg ctcatcccgc aggcctcgct ggcggtggag
     1501 tcggctacat cagcttacga ggtaggcaac ctggattttc taagcttgct ggcaaatctg
     1561 acgaatctga tcgagctgca attacagtac tactacgagg tagagcggca cgaggaggcc
     1621 atcgcccgcc tcgaaccgat tgccggcagg gagctcattc ccttcgggag gacgaactga
     1681 tgagcaagca acgcattcga accctcgcgg tgggggtgct cgtggcggcc gctgcgggcg
     1741 cgctggtgta ctttggcgct ggccgacggc ggcctgtgca ccgcaacgca gccggaacgg
     1801 cggccgcgcg caatatttac tactgcccta tgcatccggc ctatcgctcc gacaagcccg
     1861 ggaactgccc gatttgctcg atgaaaatgg tgaagttgga agaacccgcc cagcccgcct
     1921 ccgtgcctgt tccaggcaca acggcgccgg cctcctcctc cgcctcggag gcggcgcaac
     1981 ccgggatctc gcaaagcggc gccattttcg tgccgcccga gaagcagcag cttatcggaa
     2041 tgcgctcggt gcgggccgaa atcggaacgc ttacgaaaga aatccggact gtcgggaagg
     2101 tgagctacga cgagacgcgg ctcacgcaca ttcacagcaa ggtttcgggg tacatcgaag
     2161 atgttttcgc cgattatgtc ggcaagccgg ttcgcgccgg agacccgctg ttcacgatct
     2221 acagtccgga cctcgtcgct accgagcagg agtatctctt ggccctgcgc tcgcggaatc
     2281 tgctacggac cagtaccttg ccctcggtcg cagagggctc ggaaaacctc gtcaccgccg
     2341 cccgagaacg cctgcggctt tgggatgtaa cggaatcaga aatccgctcc cttgaaacgg
     2401 agggcaaagt tcagcgcgcg attaccgttt attctcccgt ggcaggcgtc gtcatcgagc
     2461 gagccgctta tcaccacggc accttcgtag atccttccaa ggacctgttt acgatcgtcg
     2521 acctctcgcg cgtatgggtg cttggcgaga tctacgaaac cgatctgccg ttcgtccatc
     2581 cgggagagat cgcggaaatt gaactgccct actctggcag aaagatccgc ggccctatcg
     2641 atttcatcta tccctttctc gatcccaaga cccgcactac gcaagttcga atacagttcc
     2701 ccaacccgaa cctgacctta aaaccggaca tgttcacaaa tatccggatt gtggttccgc
     2761 tcggccggca agtgctggtt cccgtggatg cagtcatgga caccgggacc gagcagtatg
     2821 tcttcatcga tgagggcgat ggctacgtcc agccgaggca ggtgaagatc ggcgccgaag
     2881 ccggcgacaa agtgggcgtt caacagggtc tgcggccggg cgaacgggtg gtcaccagcg
     2941 cgaactttat catcgattcg gagagccggc tgcaaggggc attcgcgaat atgggccggc
     3001 cttccggacc tttcgcctcc gccgcctccg gtggcgggca agccgtcgcc gttgaggtgc
     3061 tggagccgaa actcgccaag acgggcagga acccgattcg tttgcgaatc aacgatggcg
     3121 ccggtaatcc agtcgccggc gcgcaggtgg aagtgacgct ctttatgccg caaatgggcg
     3181 cgatgccgcc catgacctcc cgggcggctc tggccgattc gggaaacggc gtctactccg
     3241 gccagatcga atttccaatg gcgtgtacct
//
LOCUS       DASREK010000042         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2331318, whole genome shotgun sequence.
ACCESSION   DASREK010000042 DASREK010000000
VERSION     DASREK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331318"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFA54_02495"
     CDS             complement(<1..886)
                     /locus_tag="VFA54_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49701.1"
                     /translation="MIVFISQTEMADSRALLAGFRRRLEEVSPNSREGVTDLLIDFGV
                     IESAIADALCPDRDAGGPVLDALRAISVELGHLFRSTPGRRAATIREALDRIAGCSLP
                     RAVRVGVPEGYSHYGVYPDMYRLAAERFWLQARPVKIVIIGIRSVGTSLSAVVAAELE
                     ARGCRARSCTVRPRGHPFDRRLHLAPEIGGLFRFSPGAFYLVADEGPGLSGSSFLSVA
                     EKLAGHGVPEERIAFFPSWDSSGAQLRSAAARERWRRYKRYSVSFEEAFPAYSRLIDV
                     SAGRWRRLFYPAGGGPAVQ"
     gene            complement(883..1623)
                     /locus_tag="VFA54_02500"
     CDS             complement(883..1623)
                     /locus_tag="VFA54_02500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS49702.1"
                     /translation="MPLEPFQDYSPFAWFYNRHWAAEYHRQALPVIDMLLLRRLPRRS
                     RILDLCCGTGQFTACLAGRDFDVIGMDSSREMLRFARQNAPGVNFIAADARRFALRRP
                     FDAVLSIFDSLNHIMTLEDLAQVFRNVRDVLRPGGVFVFDLNRREAFELFWSQTHAIV
                     EDDNICITRGSYSSRERTARCDITMLRLLDGWRRADAVIYQKCHPIEDVRCELGRAGF
                     GETQIYDAHQELGMPEEMGWARTFFVAS"
     gene            complement(1613..2653)
                     /locus_tag="VFA54_02505"
     CDS             complement(1613..2653)
                     /locus_tag="VFA54_02505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS49703.1"
                     /translation="MQVGEINGIEDLEALRLEWSRLWERCPHATIFQTPEWLLPWCRN
                     LFGGGSLWSLAVWRDGRLAALAPMFIWGGERKCISMTGAGVTDYLDFLCEPDVAAPAV
                     QMILGRLGQREDNWRVCVLDDLPPESPLLARGAIPESLAWRRESCANCPVLDLPATVE
                     ELYRRLPHKFQVDLRRAKNRLARAGKLEVELAGEPTLPWCLASLFELHQARWQARSQT
                     GMLASGALQAFHREAAAGFLRRGALRLWVLRLNGAAAAVLYAFARWPRLYIYLGGFEP
                     SLSRLSPGTVLMSLAIEQAICERFQQLDFLRNPESFKRLWGARDRWSQRLVIRRSGAA
                     AGGVGAGVADAA"
     gene            complement(2663..>3313)
                     /locus_tag="VFA54_02510"
     CDS             complement(2663..>3313)
                     /locus_tag="VFA54_02510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49704.1"
                     /translation="NTYLHCVSESQHRTRPERSSMLAPIANGVPVERLAANARKRKFA
                     LALGRICPEKGFHLAIDAAGRAGVPLRIAGQVFGYPAHVKYYREQIAPRLDGRQVRFL
                     GPVGFESKRRLLAAARCLLAPSLVSETSSLAAMEAIACGTPVIAFPSGALAEIVEDGR
                     TGFLVRGAAEMADAIRAAGSIEAEECRRAARERFDSRRTIENYLTLYETLAALRAG"
BASE COUNT          680 a         1107 c          995 g          531 t
ORIGIN      
        1 cctggacggc gggtccgccc cccgcggggt agaacagccg ccgccatctc ccggccgaca
       61 cgtcaatcag ccgcgagtac gccggaaaag cttcttcaaa actcacagag tatcgcttat
      121 accgcctcca ccgctctcgg gccgcggcgg atcgcagttg ggcgccgctg gagtcccagc
      181 ttggaaagaa cgcaatccgt tcctcaggaa cgccatgccc cgccagcttc tccgctacag
      241 aaagaaacga agatccgctc agtccggggc cctcatccgc cacaagataa aatgcgcctg
      301 gcgagaagcg gaaaagcccg ccgatttccg gcgccagatg caggcggcgg tcaaacgggt
      361 ggccgcgcgg cctcaccgtg caggagcggg cgcggcagcc gcgcgcttcc agttcggccg
      421 ctaccacggc gctcaggctc gttcccacgc tgcggatacc tattataact atcttgacag
      481 ggcgggcttg gagccaaaag cgttccgctg cgagccggta catatcggga tacacgccgt
      541 agtgcgagta tccctccggc acgccgacgc ggacggcccg cggcaggcta cagccggcga
      601 tccgatccag agcttctctg atggtcgcgg cgcgccgccc tggcgtgctc cggaaaagat
      661 gccccagttc tacgctgata gcccggagcg catccagcac cgggccgccg gcatcgcgat
      721 ccggacacag cgcatcggcg atggccgact cgatcacgcc gaagtcgatc agaagatctg
      781 ttacgccctc gcgggaattg ggagaaactt cttccagtct gcgccggaac ccggccagca
      841 gagccctcga atcggccatt tccgtctgcg atatgaagac aatcacgaag ccacaaaaaa
      901 agtgcgagcc cagcccatct cctccggcat gccgagctcc tggtgtgcgt cgtagatttg
      961 cgtctcgcca aatcccgccc tgcccaactc gcagcggacg tcctctatgg gatggcattt
     1021 ctgataaatc acggcgtcgg cgcgccgcca tccgtcgagc agccgcagca tcgttatatc
     1081 gcagcgcgct gttctttcgc gggaagagta actgccccgg gtgatgcaga tattgtcgtc
     1141 ttccacgatg gcatgcgtct gactccagaa caactcgaag gcctcgcgcc ggttcaggtc
     1201 aaagacaaag acgccgccgg gccgcaggac atcccgaacg ttccggaaca cctgcgccag
     1261 atcttccaga gtcatgatgt gattcaggct gtcgaatatg gaaaggacgg cgtcaaaagg
     1321 gcggcgcagc gcaaaacggc gcgcgtcggc ggcgatgaaa ttcacgccgg gcgcattttg
     1381 gcgcgcaaac cgcagcatct cgcgggaaga atccatgccg ataacgtcga aatcgcggcc
     1441 ggcgaggcag gctgtgaact ggccggttcc gcaacagaga tccagaatcc gcgagcggcg
     1501 cggcagccgc cggagcagga gcatatcgat aacaggcaac gcctgccggt gatattcggc
     1561 ggcccagtgc cggttataaa accaggcgaa ggggctgtaa tcctggaacg gctcaagcgg
     1621 catcggccac gcccgcgcca acacctccgg ccgctgcgcc ggagcggcga ataaccagcc
     1681 gctggctcca gcgatcccgg gcgccccaga ggcgcttaaa gctttccggg tttcgcaaaa
     1741 aatcgagttg ttggaatctt tcgcaaatcg cctgctcgat cgcgaggctc atcagcacgg
     1801 ttccgggact gaggcggctc aaagaaggct cgaagccgcc gagataaata tagagccggg
     1861 gccagcgcgc aaaagcatag aggaccgcgg ccgccgcgcc gttcagacgc agcacccaga
     1921 gccgcaaggc tcctcgccgc agaaatccgg ctgctgcttc ccgatgaaat gcctgcaatg
     1981 cgccgctcgc cagcataccg gtctggctcc tcgcctgcca gcgcgcttga tgcagctcga
     2041 agagcgatgc gagacaccag ggcagcgtcg gttcgccggc gagttcaact tccagttttc
     2101 cggcccgcgc cagccggttc ttcgcacgac ggaggtcaac ctgaaatttg tgaggcaagc
     2161 gccggtaaag ctcctcgaca gtcgccggca gatccagaac ggggcagttc gcgcagctct
     2221 cccggcgcca ggccaaactt tctggaatgg ccccgcgcgc cagaagcggg gactcgggcg
     2281 gcaggtcgtc caacacacac acgcgccagt tgtcttctct ctggcccaag cgccccagaa
     2341 tcatctgaac ggcgggggcc gccacatcgg gctcacaaag aaaatccaga tagtcggtca
     2401 cgccggcgcc ggtcatcgag atgcatttcc gttccccgcc ccagatgaac atcggcgcaa
     2461 gcgcagccag gcgcccgtcg cgccaaaccg caagcgacca aagtgatccg ccgccaaaca
     2521 gatttcggca ccatggcagc agccattcgg gcgtctgaaa aatagtggcg tgcgggcagc
     2581 gctcccaaag cctcgaccac tccagccgca gggcttccag atcctcgata ccgttgatct
     2641 ccccgacctg catgcgctct cttcatccgg cccgcagggc cgccaacgtt tcatacaacg
     2701 tcaggtagtt ttcgatggta cgccgcgagt caaagcgttc gcgcgccgcc cggcggcatt
     2761 cttccgcctc gatcgagccg gcggcgcgaa ttgcatccgc catctcagcc gcgccgcgga
     2821 ccagaaatcc cgtgcgcccg tcttcgacga tttccgccag ggctccggaa gggaaagcaa
     2881 tcacaggagt gccgcaggct atcgcctcca tcgctgccag cgagcttgtc tcggaaacca
     2941 ggctgggcgc cagaagacat cgggccgcgg caagcagccg ccttttgctt tcgaatccca
     3001 cgggccccaa aaaccggact tgacgcccgt cgagccgcgg cgcaatctgc tctcgataat
     3061 atttcacgtg agcgggatag ccaaagacct gtccggcgat gcgcaggggc acgccggccc
     3121 gcccggctgc gtcgatcgcc agatgaaagc ctttttcagg gcaaatccgc cccagcgcca
     3181 aggcgaactt tcttttgcgc gcgttggcgg cgagccgctc gacaggaacg ccgtttgcaa
     3241 tgggcgcgag catggacgag cgttccggcc gcgtgcgatg ttgggattcg gagacgcagt
     3301 ggagataggt att
//
LOCUS       DASREK010000043        17398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1343110, whole genome shotgun sequence.
ACCESSION   DASREK010000043 DASREK010000000
VERSION     DASREK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17398
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343110"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFA54_02515"
     CDS             complement(<1..484)
                     /locus_tag="VFA54_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HZS49705.1"
                     /translation="MWEQNYRPVAGSLGLSALVAAIPLFVLFYMLGVKRKPSWIAGLS
                     ALAAAWVVVLAVIHMPVQQALASTIYGAAFGIFPIFWIVFSAILLYRVTVDTGKFEII
                     KDSVGALSDDRRLQALLIAFSFGAFLEGASGFGTPVAVAAAMMTGLGFSPFFAAGICL
                     L"
     gene            930..1286
                     /locus_tag="VFA54_02520"
     CDS             930..1286
                     /locus_tag="VFA54_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49706.1"
                     /translation="MRKIALTAVLSVVATLVSVHYVPAANLPTITKLENPKVTVTEMD
                     YLRGVPREPYVRPTDQVIVFLDDATYDRIDPATGAKQRVTRKSGDVIWHDKGDHAPRL
                     INMGAKTYRTLIIALK"
     gene            complement(1326..2819)
                     /locus_tag="VFA54_02525"
     CDS             complement(1326..2819)
                     /locus_tag="VFA54_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HZS49707.1"
                     /translation="MAVQPYRTQARAGHRILLTPLSSIDDRPPQLDPMLSRWRLLAVI
                     ASGIGIWTLPRPEDIPSQGWHLLAIFAATILGMILQLMEAGAVVLLGLTAAILTGAMT
                     VGAVLGGFSNSIVWLIVSAFLFSQAVTGTGLGRRLAYLFIQAFGHRTLGLGYALVASE
                     MVIAPAVPANTARAGGILFPIASSIARACGSSPKESPRLGAFLMLNQFHATIILSAMF
                     LTATTTNPLVAELAAKAAGVKISWGLWALAGAVPGAISLVLAPLVLYRLHPPETAESP
                     EAAAEASRRLEELGPMSRAEIILAVVVGCCLMLWCTTALHKLDPTAIAFAGLAAMLLG
                     GVLKWQDVLSTRGAWDAMLWFAGLIGMTDWLGRLGVTAWFARSVAGHVHGRWFWILLI
                     LSLVYFYSHYAFASMTAHVTAMFTPFLGVAVAGGAPALLATLLLGFFSTLNAAMTHYS
                     TGPAPIYFGAGYVSQAVWWKAGFALSVLYVVIWAGVGLPYWKLIGIW"
     gene            complement(2847..3287)
                     /locus_tag="VFA54_02530"
     CDS             complement(2847..3287)
                     /locus_tag="VFA54_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS49708.1"
                     /translation="MKYFLALTVFLTAAVLLSAAEGTTADVSYVPHDKVAAVLQKGGS
                     MVNQSDLAVQGTHRVKPGGAEVHLKETDVFYVVDGEATVVTGGTVLNQRTGKNPNQIG
                     GSGIEGGHEYHISKGDVMVIPAGVPHWFKETNGIVYYVVKVKKP"
     gene            complement(3410..3979)
                     /locus_tag="VFA54_02535"
     CDS             complement(3410..3979)
                     /locus_tag="VFA54_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49709.1"
                     /translation="MSRLAIAALCWAACLGASPAISPEKSHSAKEKLEQIAEGKLKPG
                     TALVLSEDEINSYLRYDYASEIPAGISEPHISLEPDRVRGSAVVDFLEWGSETGAAPG
                     PLLSWMLRGKRPVEAVCHYASSDGYGQVDVESVKIGGVSLSAAAITFLIDNLVTPRFP
                     GAVVGRRAPLGFHLRQVRIEQGRAVFVAR"
     gene            complement(3976..6426)
                     /locus_tag="VFA54_02540"
     CDS             complement(3976..6426)
                     /locus_tag="VFA54_02540"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZS49710.1"
                     /translation="MNHGSAAHSGMRLLSKILLWAAALLLFAGLVVPLIHANRFHRQI
                     RAALESALGRKVEIGEVTLNLLTGPGFQLQNVIIGEDPAFGIEPFAYMDSMQVRLRLR
                     TLWTGQIQLASLTLINPSINLVKNAGGRWNFEALLERARGGTAPARQGGGFETYFPYI
                     GVQSGRLNFKFGDYKSVFYIQDLEAALAPLRDEGDRWRTRFAGTPARTDRTLSGMGRV
                     TGEGDLGAAAGLIRLDLALESSPLEHLLALVYGRDFGIHGEASARAQLSGEMSNVRIQ
                     GVLQAGDVHRWDLLPSQESRFAAPFLGQLDLPHQKLEIQTSPSAQIPVRAHFTLQNYL
                     SEPVWNAVLELDRAPAEPFLRTAQHLGVELPGGLEVRGYLSGRTEFHGSLWPQGSLRF
                     QNGQVLLQNSRPLAVESAEIRLDGATFEIPPAPMNLGEEVLQASAAGSLSPLRVEGQL
                     STQGISIETLREQFPAKRPAWIRSLASGFWEGQIAYRKQGAAPGILTGSGLLTNARWQ
                     PEGLESAIEIARAKIRWEPKAVHIDELTGSLGESSFTGSCRSRTAEDGSGAGQNFCAV
                     HFPELDVSELDQWLNPQQKRSRWEIWKRALGRSDGDAAAWLQAARMQGNLTIDTLTAG
                     NWTFRNVRSDVVCGGGSLALNRFRAELGKGLVRGTFRVEFLKPAPRYQLLASVRGIDL
                     KSLSDSAVLPANFQHGALDLNLELSTRGRTAAELRAALKGAGAFSGHSLTLENVEWNE
                     AAAEADSLEIRSLDGRFELTRNGLNLKDLNMVVGGEKYQGRGSVGGRPAVLLEFASHG
                     RQSRLIGAAMEETAAGTP"
     gene            complement(6423..7202)
                     /gene="rnc"
                     /locus_tag="VFA54_02545"
     CDS             complement(6423..7202)
                     /gene="rnc"
                     /locus_tag="VFA54_02545"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZS49711.1"
                     /translation="MPEDISRLETELGYQFTNPALLTRALTHSSHIHETLGRKNGFDN
                     ERMEFLGDSVLGFVVSARLVETYPEYREGKLSKLKAHLVSAAHLVETARRLRLGDYLQ
                     LGRGEELSGGRGKRALLVNALEAVIAALYLDGGVEVARRFVDRFIIGESPAEQEPADE
                     LFEDYKSALQEFLQAAKLPQPKYLVVKEQGPQHHKTFTIEVRIGNDFSAQAEGPAKKA
                     AAQEAARLALEHFRTMYGPATPHAGVLQRRQTSETPARLAE"
     gene            complement(7315..8124)
                     /locus_tag="VFA54_02550"
     CDS             complement(7315..8124)
                     /locus_tag="VFA54_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49712.1"
                     /translation="MTKTILAIAAAIWIASAAWAQQKQPKVKSRKEAEAYNAMVSAPS
                     PDARIQAAEDFLTKFADSDFKEMALQTETLAYQQKNDFDNMVIFGERTLEVNPDNIEV
                     LVALARGIAQRTREHDLDRDEKLARAEKYAKRAQTLIPNWSKPNPQIPDEQWTEFKKD
                     RMAQAHAALGQVAFVRKDFANAEQSFKAGVDVAPQPDPVLLYQLGMVYGAEAKYDEGI
                     ATMDKAIAAGGVKVGGRDLAAEQKANFEKAKASAASKSSAAPGAPPQTKQQ"
     gene            complement(8177..9670)
                     /locus_tag="VFA54_02555"
     CDS             complement(8177..9670)
                     /locus_tag="VFA54_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS49713.1"
                     /translation="MSRFFVSLLALVLAAPGARALAAEQGQLDSDITLFTVMAAINAA
                     GYDAGLDSPAGSPVRAAVRRDLRVFQGPTLEALKNFYQQHKPGDPVQDLSQYVSFALQ
                     CAGPPDFKLRGPFTDLSPDAQPLAGLSLLLASFYREADIQKLYEKYQPDFEKEIERYH
                     EPITQAIWETNGYLRIPTSGYLDRRFQIYIDLLSAPNSPSVRGYGATVFVVLHPSREL
                     RTREVRHAYLHYLLDPFASKYAAAVNAKKQLAGLAMFAPALDESYKTDFSLLVTECLI
                     KAVEARMRYGSEKQKQADVERAVQEGYILAPHFYEQLLRYEAQEQGIRLYYPDLISSI
                     DVKKEDQRLRRVHFVERAPEPKHPVEAVQPPALTGAAKLLQQAEESLSRHEIDQAKEL
                     FLQAQQQSGDNKEPRALYGLARVAALEKDPERAKELFREALDASPDPHVRAMSHIYLG
                     RIEDLLGNRESALEHYKEALAAGDTTPGTREAAEKGLKESFTSPKSE"
     regulatory      10026..10215
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10318..12522
                     /locus_tag="VFA54_02560"
     CDS             10318..12522
                     /locus_tag="VFA54_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49714.1"
                     /translation="MKCLFLRGGVAVLSLASTAALCRAAIQGTVQDSSGAAVPHARVY
                     VLQQPFPSRAAAADSAGAFRFPDLGAGRCTVFASAPGLSGDMVQTPCDSSEPLVLTLH
                     PSALAETVVVTAERTDLPSAEAASSTSVVTADDVRAMQAMELFEAFRYLPGVEVNQTG
                     RYGGVTGIFIRGAGSPGSSNYNLVAVDGVPINDVGGAYNFASLPPELIDRVELVRGPQ
                     AALYGPYGIGGAVQVITPSGLEHHDLFSSVEGGNFGTRRLAVGGGGQIGDFGFYGSLS
                     RLQSDGMAANDDSRIDNAHLKADYVLHRRHHFQYSLLADSNESGNPGAFGSDPAHLFP
                     GIDTVSRTFENYDVHAFHYDAQFGSRVNQRLTGGVYSDRLDFHSRFGPSFSRQSRNTV
                     SSETSLILAGHDVLIFGMDWSGERFRNTFLTDAGSAFFPVERSFFGWFVENHFEWRGK
                     LFLNAGARLEYIRRNAIPPDAFGSRPFLSEDREAEAHPKLSAAYLLLATTRLHGSAGT
                     GLRPPNGFELTFTTNPALRPERTVSFDAGVAQSFWDRRITADVTWFYSRFRDQIVTLS
                     PAAQSLSRWTSDNLANSEAGGVEAALYIDAGKNLRLRGAYTYLDTAVLSLDGSSTLVQ
                     GIFRLGQQLIRRPRHSGSYMAVWQRRRLLVETGAVLHGRTLDVEPNYGAGEYLNPFYA
                     TFDAGAQFEISRGIALTAKVRNLLDRTYEESLGFPALGRNFTVGVKWRWLRE"
     gene            12801..14189
                     /locus_tag="VFA54_02565"
     CDS             12801..14189
                     /locus_tag="VFA54_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49715.1"
                     /translation="MKLASLPLLLLTPAAAQQAPALSAAGLAIAASQQLQNVAWGAAY
                     SGWRRDHSQLACRQFPQGEHATLPDALWSHRCEQTAQGVKAEWLFYALNLDEPITPRL
                     EQFRAIVEGMPPASLEEIRRELSSRLSNRYGAAEKIDAPREPLAEPGSVFWRNWQRWR
                     SGDLELYLYLFEAQRQAPRLELQARRRPLLEALALERRLQESDLGRWSEAGTPLDLRL
                     AKELGALFPGLSRLLASRQPERDQNTLRPAVIELLEAANTASPERRAMLLLAADRLAG
                     RLGPTERESPQWEQERKQLAAYGLNFEWDELGGSWAYTHDLLWRVWEDYAGMPWGEQA
                     FLLLESRGWNTHVGCQAGSDEFRMVIQTGERFLREYTESQNRPDVLLFVAQSYETWWS
                     LSQAGERDDYVDPAKYQQGAGAARERAIRLYEQLLATAPDGDGAARARQQLPRLKLGI
                     DTVQRRFFCVYD"
     gene            complement(14241..15170)
                     /locus_tag="VFA54_02570"
     CDS             complement(14241..15170)
                     /locus_tag="VFA54_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49716.1"
                     /translation="MFVLVLLLLALGPAAAQEILLYPEFQRPSPRGDIVPADRGGRQW
                     EIISPAMPRNGHAGFFVAARLREPGFFRLDIAMNPVGRLRADLYRVWFGDPDASGERY
                     PGRLTLVGQTVAGRFPDDEQSAPDPPEHQPAPASPASIPARPRADAAGIYWLDLYAPR
                     DLPSGRIRLEVVLQSAGPRVVYPMEVRILPIAFPDRKGASDWKGAKAAGTQPDQTRAA
                     QPAQTIALQFFSAWWNAQPLPLPASRNEPLTVESRIERDVLQDLELLESLAPKLGRDK
                     LRADVSRLMAGDNLPPDRWLEVRRWIYRTAQGR"
     gene            complement(15221..15979)
                     /locus_tag="VFA54_02575"
     CDS             complement(15221..15979)
                     /locus_tag="VFA54_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49717.1"
                     /translation="MRILALLAFSTTALSFAQQPPLVIPHFADGGGWKSTIAVFNNGV
                     NVTPVHLTIKFRGDDGALVPVDLNNYGSLSTLDTDLGVQSALYLDTAGTRPAVQVGWV
                     EVDQTTGSGAVAGFVIFRNIVPGRPDFEAVSNGMRAASSMTFPFDNTNGVVTTFAIAN
                     LSVSTCTVAAFPIFDESGIAVVGQPQLIGNMYGSGHMGFIATDRIPEMGNKRGHLTFN
                     PLIGCGAGGIAMLGLRFNPSGAFTNLEPLSVTTP"
     gene            16285..>17398
                     /locus_tag="VFA54_02580"
     CDS             16285..>17398
                     /locus_tag="VFA54_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49718.1"
                     /translation="MKTLLFLFLLLAIAAAAAPANMPNLAELQRMMARFAPVELRVDT
                     SALSANDRQALLKMIQAARVMNDIFLKQIWSGNPELYARLQKDHTPLGRARLHYFWIN
                     KGPWSELDGHRAFLPGVPPRKLPGANFYPEDMTKEEFEAWVKGLPPAGRQAAEGFFTV
                     IRRDSSKNLTIVPYNKEYAADLERAAALLRDAAGLTGNESLKRFLNLRAGAFLSNDYY
                     ASDAAWMDLDAPLEPTIGPYETYNDETFGYKAAFEAFINLRDEKETGRLQFFAKHLQE
                     VEDNLPIEPRYRNPHIGSLAPIRVVNEIYAAGDAAHGVCTAAYNLPNDERLVNEKGSK
                     RVMLKNVQEAKFRTTLVPIAGRVLAKSAQADVNFDSF"
BASE COUNT         3405 a         5479 c         5237 g         3277 t
ORIGIN      
        1 ccagcagaca gataccggcg gcaaagaatg gactgaaccc gagcccggtc atcatcgccg
       61 cggcaactgc cacgggcgtt ccaaatcccg acgcgccttc aagaaaggcc ccgaatgaga
      121 aagcaatcag cagcgcttgc agccggcggt cgtcgctcag cgcccctacc gagtctttga
      181 tgatttcgaa cttgccggta tcgaccgtga cccggtaaag caggatcgct gagaacacga
      241 tccagaatat ggggaaaatt ccaaaggcgg cgccatagat cgtggatgcc agcgcctgct
      301 gcacaggcat gtgaatcacc gcgagcacga ccacccaggc cgcggcgagc gcggaaagac
      361 ccgcaatcca gctaggcttg cgctttacgc ccagcatgta gaaaagcacg aacagaggta
      421 tggcagctac caacgcggac aatcccaggc ttccggcgac cggccgataa ttttgttccc
      481 acataattcg cctcgcgatc agagtcgaaa cggaaactga gactttatca caatttttca
      541 gccgggcaag tcttgcggcc agaaaaacac ccctcgggcg ttgcgtggct ccggcactgc
      601 aagtgtaccc gctgctcgcg ccgggcgccc ggcggctggt atcaaaagct actgttagcg
      661 ccggttcatg attcggactc ggcgcagccg ccgatgcagt gcgccggaac cggtgaagac
      721 ataaggtctt gggccggtcc ttcgcctcgc ggattcggct ctgcggggcc ccggcacggt
      781 cgggaaaagg cgggggcgtg tcgtccgggg actgcgaaat gccacggtgg ctgaggcccc
      841 gatcgcgcca tttcttcgtt cggaagcctt tccctcacag gccagtcatg gcaagatgca
      901 gggaggcaga tccgaatgga ggctgtttga tgagaaaaat tgctctaacc gcggttctct
      961 ctgttgtggc caccctcgtc tccgtccact acgttccggc ggcgaacctg cccacaatca
     1021 caaaactgga aaatccgaag gtcaccgtca cggaaatgga ttacctccgc ggggtgcctc
     1081 gggaaccata cgtgcggcca accgatcagg tgattgtgtt tctggacgac gccacctatg
     1141 accgtatcga cccggccacc ggcgccaagc agcgcgtcac gcggaaatca ggcgatgtga
     1201 tatggcacga taagggcgat cacgctccgc ggctcatcaa tatgggagcc aaaacctacc
     1261 ggaccctgat tattgccttg aagtaagcgc gcgccggacg caaatatggc aggcgacgtg
     1321 ggcggctacc agatcccgat gagcttccaa tacggcaggc ccactccggc ccaaatgacg
     1381 acgtacagga ccgatagcgc gaacccggct ttccaccaga cggcttggct tacatagccg
     1441 gccccgaaat aaatgggagc cgggccggtc gagtagtggg tcatagcggc gttcagcgtg
     1501 gaaaagaacc caagcaacag cgtggccagc agagcgggcg ctccgccggc cacggccacg
     1561 cccagaaacg gcgtgaacat cgccgtgaca tgcgccgtca tgctggcgaa ggcgtagtgg
     1621 gaatagaagt agaccaggga gagtatcaat agtatccaga accagcggcc gtgcacatgg
     1681 ccggcgacgg agcgggcgaa ccaggccgtg actccaagcc ggccgagcca gtcggtcatg
     1741 ccgatcagac cggcgaacca cagcatcgcg tcccaggcgc cccgcgtgct cagcacatcc
     1801 tgccatttca gcaccccgcc gagcagcatc gcagcgaggc ccgcgaaggc gatagccgta
     1861 ggatccaact tatggagagc ggtcgtgcac caaagcatca gacagcagcc gactacaacc
     1921 gccaggataa tttcggcccg gctcatcggc cccagctcct ccaggcgccg gctcgcctcg
     1981 gccgcggcct ccggagattc cgccgtctcg ggcggatgca ggcggtataa gaccaatggc
     2041 gccagcacga gagaaatcgc gccggggacg gccccggcaa gcgcccacag accccaactg
     2101 atcttgacgc cggcggcctt cgccgccagc tctgctacca gtggatttgt tgtggtggcg
     2161 gtcagaaaca tcgccgagag aatgatcgtg gcatgaaact ggttgagcat taagaatgct
     2221 ccgagccgcg gcgactcttt gggcgatgac ccgcacgccc gcgcgatgct gctggcaatc
     2281 ggaaaaagga tcccgccggc ccgcgccgtg ttggccggta cggccggtgc gatcaccatc
     2341 tcggatgcca ccagcgcata gcccaagccg agcgtccgat gtccgaaggc ttgaatgaac
     2401 agataagcca accgccggcc aagccctgtc ccggtcacgg cttgcgagaa cagaaaggcg
     2461 gaaacaatca gccacacgat cgagttgctg aaaccgccca gcactgcgcc gaccgtcatc
     2521 gctccggtca ggatggcggc cgtgagaccc agtagaacga cggcgccggc ctccataagt
     2581 tgcaggatca tgccaaggat ggtcgcggcg aagatggcca gcaaatgcca cccctgggaa
     2641 gggatgtcct cggggcgcgg aagcgtccag attccgatac ccgaggcgat tacggccagc
     2701 agacgccacc ggctcagcat ggggtcgagt tgcggggggc gatcatcgat ggaagagaga
     2761 ggcgtgagaa gaatgcggtg gccggcccgg gcttgtgttc tgtacggctg gaccgccacc
     2821 gcctgaggtg aacagaaaat cttgcgttac ggctttttca ccttgacgac gtagtacact
     2881 atcccgttcg tttccttgaa ccagtgcggt acaccggccg ggatcaccat gacgtcaccc
     2941 ttactgatgt gatattcgtg gccgccttcg atgccgctgc cgccaatctg gttcggattc
     3001 ttccccgttc tttgattcaa aacggtgcct ccggtgacca ctgttgcttc gccgtccacc
     3061 acatagaaca catcggtttc tttaaggtga acctcggctc cgccgggttt cacgcgatgg
     3121 gttccttgaa ccgccagatc cgactggttc accatcgagc cgcctttttg cagcacggcc
     3181 gcgaccttgt cgtggggcac atagctgacg tcggctgtgg tcccctcggc tgcgctgagc
     3241 aaaacagccg cggtgaggaa tacggttagc gccaagaaat acttcatctc gccctcctgt
     3301 tgattcgcct tctcttacgc ctgtctgaca aacagacgta tcatacgcca ggcgggtatg
     3361 tggtgtccat tttggccggc cggaaaaaga cggcgcccgg accggagggc tatcgggcta
     3421 cgaacacggc cctgccctgc tcgatgcgca cctgcctgag atgaaagccc aacggcgcgc
     3481 ggcggccgac cacagcgccg ggaaatctcg gcgtaaccag gttgtcgatc aaaaacgtga
     3541 tcgctgcggc ggaaagcgat acgccgccga ttttgacgga ttcaacgtca acctggccgt
     3601 aaccgtccga agacgcgtag tgacacacgg cctcgacggg gcgcttgccg cgaagcatcc
     3661 agctcagcaa aggccccggc gcggcgccgg tttcgcttcc ccactccaga aaatcgacca
     3721 ccgcggagcc tcttacccga tccggctcca agctgatgtg cggctcgctg atgcctgccg
     3781 gaatctccga agcgtaatcg tagcggagat aggagttgat ctcgtcttcg ctgagaacca
     3841 gcgccgtgcc gggtttcagc ttcccttccg cgatttgctc gagtttctcc ttggccgagt
     3901 gagatttttc gggagaaata gctggagagg ctcctagaca cgctgcccaa cagagcgccg
     3961 cgattgccag gcggctcatg gcgtcccggc cgcggtttct tccatcgccg cgccgatcag
     4021 ccgggactgc cgcccgtgcg acgcaaactc caggagaacc gccggtcggc cgccgaccga
     4081 tccgcgccct tgatattttt cgccgcccac caccatattc aaatccttca gattgagccc
     4141 gtttcttgtc agctcaaagc ggccgtcgag cgaacgaatt tccaggctgt ccgcctccgc
     4201 cgccgcttcg ttccactcga cgttttccag agtaagggag tggccggaga atgcgccggc
     4261 gcccttcagc gcggcgcgaa gttcggcggc tgtccgcccg cgggtgctca attcgagatt
     4321 gagatcaagg gccccgtgct gaaagttcgc aggcaggact gcggaatcgg aaagactctt
     4381 cagatcgatg ccgcgcacgg acgccaggag ctgatatcgc ggcgcaggtt tcaaaaactc
     4441 aacgcgaaag gttccccgga ccagcccctt gcccaattcc gcgcggaagc ggttcaaggc
     4501 aagcgagcct ccgccgcaaa ccacatcaga ccgcacattg cgaaaggtcc agttcccggc
     4561 ggtgagcgtg tcgatcgtca gattgccctg catccgggcg gcttgcagcc acgctgccgc
     4621 atccccatcg gagcggccca gagcgcgctt ccagatctcc caacggctgc gcttctgctg
     4681 ggggttcagc cactgatcca gttcgctcac atccaattcc ggaaaatgaa cggcgcaaaa
     4741 attctgcccc gccccggaac catcttcagc cgtgcgagaa cggcagcttc cggtgaacga
     4801 cgattctccc agcgagccgg tcaattcatc gatatggacg gccttcggct cccatcgaat
     4861 tttcgctctg gcgatctcga tcgccgattc caggccctcg ggctgccagc gggcattcgt
     4921 gagtaagcca gaccccgtca aaataccggg ggccgcccct tgtttccggt aagcgatctg
     4981 gccctcccaa aaaccggagg caagcgaacg gatccaggcc ggacgtttcg ccgggaactg
     5041 ctcgcgcagg gtctcgatgc tgattccttg cgtgctcaac tggccttcta cccgcaacgg
     5101 actcaggctg ccggcagcgg acgcttgcaa aacctcctcg ccgagattca tcggcgccgg
     5161 agggatctca aacgtcgcgc cgtccagccg gatttcggcg gattccaccg ccagcggccg
     5221 cgaattttgt aaaagcacct gcccgttttg aaaccgcagc gacccctgcg gccagagcga
     5281 gccgtggaac tccgtccggc cgctgagata gcctcgcact tcaagaccgc ccggcagctc
     5341 gactccgagg tgctgggcgg ttcggagaaa cggttccgcc ggcgcgcggt ctaattcaag
     5401 aaccgcgttc cagaccggtt cagataagta attctgcaac gtgaagtggg cgcggacggg
     5461 tatttgcgcg gaggggctgg tttggatttc cagtttctgg tgcggaagat cgagctggcc
     5521 taaaaagggc gcggcaaacc ggctctcctg cgagggcagc aaatcccagc gatggacgtc
     5581 tccggcctgt aaaacgccct ggatccttac attcgacatt tcgccgctca attgcgcccg
     5641 cgcgctcgcc tctccgtgga tgccgaagtc gcgtccatag accagggcga gaagatgctc
     5701 gagggggctc gactcgagag ccaggtcgag gcggatcagg ccggcggccg cgcccaagtc
     5761 gccttctccc gtaactctcc ccatgccgct caaggtgcgg tcggtgcgcg ccggcgtccc
     5821 ggcgaagcgt gttcgccacc ggtcgccttc atcgcgcaag ggagcgaggg ccgcctccaa
     5881 atcctgtatg taaaagacgc tcttgtaatc gccaaatttg aaattgaggc gtcccgattg
     5941 aacgccgata tacggaaagt acgtctcaaa accgcctcct tgccgcgccg gagccgttcc
     6001 cccgcgagcc cgctccagca gcgcctcgaa attccagcgc ccccccgcgt ttttcaccag
     6061 attgatgctg gggttgatga gggtcaaaga ggcgagctgt atttgcccgg tccaaagcgt
     6121 tcggaggcgc agccggacct gcatcgagtc catataggcg aacggctcaa tgccgaatgc
     6181 gggatcttcg ccgataatga cattctgcaa ctgaaagccc ggacccgtaa gaagattcag
     6241 cgtgacttcg ccgatttcga ccttccggcc caaggcgctc tcaagcgccg cccggatctg
     6301 ccggtggaaa cgattggcgt ggatcagtgg aaccaccagc cccgcgaaaa gcagcagcgc
     6361 ggcggcccaa agcagaatct tggaaagcag ccgcatgccg gagtgggccg cggaaccgtg
     6421 gttcattcgg ccaacctcgc gggcgtttcc gaagtctggc ggcgttgcag aacaccggca
     6481 tggggcgttg ccgggccgta catcgtgcgg aaatgctcca gagccaggcg cgccgcctcc
     6541 tgagccgccg ctttcttggc gggtccctcg gcttgggcgc tgaagtcgtt cccaatgcgc
     6601 acctcgatgg taaaggtctt gtggtgctgc ggcccctgct ctttgaccac caagtacttg
     6661 ggttgcggca gtttcgccgc ctgcaaaaat tcctggaggg ccgatttgta gtcttcaaaa
     6721 agctcgtcag ccggctcttg ctcggccggc gactctccaa tgatgaagcg atccacgaac
     6781 cgccgcgcca cttcgacgcc tccgtcgaga tagagcgccg ctatgacggc ctccagtgcg
     6841 ttaaccagca gcgcgcgctt gccgcgccct ccgctcaatt cctcgccgcg gcctagctgc
     6901 aaataatcgc caagccggag acggcgcgcc gtttccacca ggtgggcggc gctgaccaga
     6961 tgggctttga gcttggaaag cttgccttcc cggtattcgg gataggtttc caccagccgc
     7021 gcgctgacga cgaaaccgag caccgagtcg cccaaaaact ccatccgctc gttgtcaaag
     7081 ccgttcttgc ggcccagggt ttcgtggata tgcgaactat gcgtcagcgc ccgggtgagt
     7141 aaagcgggat tggtaaattg atagcccagc tccgtttcga gccgggaaat gtcttccggc
     7201 atggtgcact cccttagtcg ttgtgcaccg tcttcggcca gcccagcggt ctgaccgacg
     7261 acgttgcaca aagctcgttt tgaaacgggg ccgcccgatg cggcccattt ctgcttactg
     7321 ctgcttcgtt tgcggcgggg ctccgggagc cgcagacgat ttggacgcag ccgaggcttt
     7381 ggctttctca aaattcgcct tctgttcggc ggccagatcc cgccctccca cctttacccc
     7441 tccggcggcg atcgctttat ccattgtggc gatgccctcg tcatacttgg cttcggcgcc
     7501 gtacaccatg ccaagctgat agagcagcac gggatccggc tgcggcgcca catcgacccc
     7561 ggccttaaac gattgttctg cgttcgcgaa gtctttccgc acaaacgcga cctggccaag
     7621 agccgcatgc gcctgcgcca tgcgatcctt cttgaactcg gtccattgct cgtcggggat
     7681 ctgcggattc ggtttgctcc agttcggaat gagggtttgg gcgcgtttgg cgtacttctc
     7741 ggcccgggcc agtttctcgt ctctgtcgag atcgtgttcc cgtgtacgct gcgcaatgcc
     7801 gcgggcgaga gccaccagta cttcgatatt gtccgggttc acctcgagcg tccgttcgcc
     7861 gaagatcacc atattgtcga agtcgttctt ctgctgataa gccagcgtct cggtctgaag
     7921 ggccatttcc ttaaaatcgg aatcggcgaa tttggtgagg aaatcctcgg cggcctgaat
     7981 acgcgcatcc gggctgggag cgctcaccat ggcgttatac gcctctgctt ccttgcgcga
     8041 cttgaccttc ggctgtttct gctgggccca cgcggcggag gcgatccaaa tggccgcggc
     8101 gatggcgaga atggttttcg tcatgttttc tcctgcggcg ccctgtttct gtcccgcgcc
     8161 ggacggcgcc gcccgctcat tccgacttgg gtgatgtaaa gctttccttt aaaccttttt
     8221 cggccgcctc acgcgtcccg ggagtcgtgt ctcccgcggc cagggcttcc ttatagtgct
     8281 ccagggcgct ctcacggttc cccagcagat cctcaatccg gcccaggtaa atatgcgaca
     8341 tggcccgcac gtgcggatcc ggcgaggcat ccagggcctc gcggaacagc tccttagccc
     8401 tctcgggatc cttttcgagc gccgccacgc gcgccaggcc atagagggct cgcggctcct
     8461 tattatcgcc gctctgctgt tgcgcttgca aaaagagctc tttcgcctga tcaatttcat
     8521 gccggctgag gctttcctcg gcctgctgga gcagtttggc cgctcccgtc agagccggcg
     8581 gctgcaccgc ctccaccgga tgcttcggct ccggggcgcg ctcgacgaaa tgcacgcgcc
     8641 gcaggcgctg gtcctccttc ttgacgtcga tgctggatat cagatccgga tagtaaagcc
     8701 ggattccctg ctcctgagcc tcgtagcgca agagttgttc ataaaagtgt ggcgccagga
     8761 tgtagccctc ctgcacggcc cgttccacgt ctgcctgttt ctgcttttcc gagccgtagc
     8821 gcatccgggc ttccacagcc ttgatgagac actccgtcac caggagggag aaatcggttt
     8881 tgtaagactc atccagggcc ggggcgaaca tcgccaggcc ggccagctgt ttcttggcat
     8941 tcaccgccgc ggcatatttc gatgcaaaag gatccagcag atagtgcagg taggcatgcc
     9001 ggacctctcg ggttcgcagc tcgcgcgaag ggtgcagaac cacgaaaacc gttgcaccgt
     9061 agccgcgcac gctcggactg ttcggagctg aaagcagatc tatatagatt tgaaacctgc
     9121 ggtccagata gcccgacgtg gggatgcgaa ggtatccatt ggtttcccag atggcctggg
     9181 tgatcggctc gtgatagcgt tcgatttcct tttcaaaatc gggctgatac ttttcgtaga
     9241 gtttctggat atctgcttcc cggtaaaagc tggcaagcag aagtgacagg ccggcgagag
     9301 gctgggcgtc cggagataaa tcggtgaagg gcccgcgcag cttgaaatca ggaggaccgg
     9361 cgcactgcaa agcgaacgat acgtactgtg aaagatcctg aaccggatca ccgggcttgt
     9421 gctgctgata gaaattcttc agagcttcga gggtcgggcc ttggaatact cgcagatcgc
     9481 ggcgcacggc cgcccgcacc gggctgccgg cgggagaatc gaggccggca tcataacccg
     9541 ccgcgttgat agccgccatc acggtaaaca gcgtaatgtc agagtccaat tgaccctgtt
     9601 cggcggccag agctctggcg ccgggcgccg ccagcacaag agccagtaaa gatacaaaaa
     9661 acctgctcaa atcagcctgc cctgaactcc ggtaacagcc acagtttaac ccaaatagag
     9721 gaggccctgc acgcggcggt ttctgcggcg cgcgccgccg gatcatctcc catttcgagt
     9781 cttcggaaat ggggaaaggc ggcggcgatt ggaaaagaac acgccggaag ggccgccggt
     9841 tccgccggca gcaccagatt taagagaggc ctgaaatcaa gccaggctcc gccagcgctc
     9901 tgaaagcctg cgccaaatcg aagtccaaca gacgagaaaa attgtcgcaa agaacatctc
     9961 gtcgcgccag cgccgccagc ggcgcacttt ggtcttgaca cgcgccgcct cgattcccta
    10021 tggtgaaaaa gcctgctaaa accgatcctg cgtgttcggt aaactggaag cccgtgaaaa
    10081 tcgggcgcgg ccccgccact gtgagcgcgg agggaaggcg cacgcaagcc actgccccga
    10141 aaagccgggg tgggaaggcg cgccggaccg tttgacgcgc gagtcaggag acagaccgga
    10201 cagcccactc ccgagcgcct gacggcttct ggggcgggcg gctgcaccgt ttgcgaaaga
    10261 gcaatctgac gcggcccgca gttctctcgg aaattcctac gcgggtagga gagagaaatg
    10321 aaatgcctct ttttgagggg cggcgtggcc gtcctctcgc ttgcgtctac ggctgccctc
    10381 tgccgggcgg ccattcaagg aacagttcaa gacagctccg gcgcggccgt accgcacgcc
    10441 cgggtctatg tattacaaca accttttccg tcgcgggcag ccgcagccga cagcgccggc
    10501 gccttccgat ttccggatct cggagcgggc cggtgcaccg tgtttgcatc cgcgcccggg
    10561 ctgagcggtg acatggtgca gacgccgtgc gattcgagcg agccgctcgt tctgactctt
    10621 caccccagcg ccctggccga aaccgtcgtg gttaccgccg aacgcaccga cctgccctcg
    10681 gccgaagccg cttcctccac ctcagtggtc accgccgacg acgtgcgggc aatgcaagct
    10741 atggagctgt tcgaagcgtt ccgctatctg ccgggagtcg aagtgaatca gaccggccgc
    10801 tacggcggcg tgaccggtat ctttatccgc ggcgccggct cgcccggttc ttccaactac
    10861 aatctggtcg ccgtggacgg cgtcccgatc aacgacgtgg gcggcgccta taacttcgcc
    10921 agcctgcctc cggaactgat cgaccgcgtc gaactggtgc gggggccgca ggctgcgctc
    10981 tacgggccgt acggaatcgg tggcgcggtg caggtgatta ctcccagcgg cctcgagcat
    11041 catgatttgt tttcgtcggt agagggcggg aatttcggca cgcgccgtct cgccgtgggc
    11101 ggcggcgggc aaatcgggga ttttggcttc tatggctccc tttcccggct gcaatcggac
    11161 ggcatggcag ccaacgacga cagccggatc gacaatgctc acctgaaggc cgactacgtg
    11221 cttcaccgcc gccatcactt ccaatattcc ttgctcgccg attccaatga atcgggaaac
    11281 ccgggggcct tcggcagcga ccccgcccat ctgtttccgg gcatcgacac cgtatcgcga
    11341 actttcgaaa actacgatgt ccatgctttc cattacgacg cccagttcgg cagccgggtc
    11401 aaccaacggc tcaccggcgg cgtttactcc gatcgcctcg attttcatag ccgctttggc
    11461 ccctcgtttt cccgccaaag ccgcaatacc gtcagctcgg agaccagtct catattggcc
    11521 ggtcatgacg tactcatctt cggcatggac tggagcgggg agcgctttcg caatacgttt
    11581 ttgacggatg ccggctccgc ttttttcccc gtcgaacgca gcttcttcgg ctggtttgtc
    11641 gaaaatcatt tcgagtggcg cggcaagttg tttctgaacg ccggagcgcg attggagtat
    11701 atccggcgca atgcaattcc accggacgcg ttcggctcgc ggccctttct gtctgaggat
    11761 cgtgaggccg aagcgcatcc caagctcagc gccgcgtacc tgctgctcgc aaccacccgg
    11821 ctgcacggaa gcgccggcac gggcttgcgt cctcccaatg gttttgagct gacgtttacc
    11881 accaaccccg ccctgcggcc agagcgcacc gtgagctttg acgcgggggt ggcgcagtcg
    11941 ttttgggacc gccggatcac ggccgatgtg acttggttct acagccggtt tcgcgatcaa
    12001 atcgtgacgc tttctccggc cgcgcaatcg ctcagccgtt ggacgtcgga taacctcgcc
    12061 aactcagagg cgggcggcgt cgaagccgct ctctatatcg acgcgggaaa aaatctgcga
    12121 ttgcggggcg cctacaccta tctcgatacg gccgtgctca gcctcgacgg ctcatccacg
    12181 cttgtgcagg ggatctttcg cctggggcag caattaatcc ggaggcctcg ccacagcggg
    12241 tcctacatgg cggtgtggca gcggcggcgg cttctcgtcg aaacgggcgc ggtgctccac
    12301 ggccgcacgc tcgatgtaga gccgaactac ggggccggtg aatatctcaa tcccttttat
    12361 gcgacttttg acgccggagc gcagttcgaa atcagccgcg gcatcgccct caccgcaaag
    12421 gtacgaaatc tgctggaccg gacctacgag gaaagcctcg gcttccccgc tctgggcagg
    12481 aactttacag tgggcgtgaa gtggcgctgg ctcagggaat aaagcgaatt cgcaatcccc
    12541 ctagggcggc tcacaatagc gcggccgccg ctcctaatca tcgcagcccg gaaatgcgag
    12601 cggcggttcc ctggagcggc cgattcgggc acgacaatcc ccatcgtagg attcatcctg
    12661 cgcgcgttcg gggacgtcgg aacgcgcgct tgttcggcct cggcctgcca cagcgtacgc
    12721 gacgccggga tcgccataac gctccattgc cctatatccc ggcgaattgg acaatgtatg
    12781 taaactatcc tgaggccgct atgaaattag cctcgctgcc attgctcctg ctgacccccg
    12841 ccgccgccca acaagcgccg gcgctttcgg ctgccggtct tgcaatagcc gcctcacagc
    12901 agttgcagaa tgtggcctgg ggcgcggctt atagcggctg gcgccgcgat cattcgcagc
    12961 ttgcttgccg ccaattcccc caaggcgagc acgcgacgct tccggatgcc ctctggtccc
    13021 atcgctgcga gcagacggca cagggagtga aggccgaatg gctcttttac gccttgaatc
    13081 ttgacgagcc gatcacgcct cggctggagc agtttcgggc tatcgtcgaa ggcatgccgc
    13141 cggcatcctt ggaagaaata cgccgcgaac tctccagccg cctttcgaat cgctatggcg
    13201 ccgcagaaaa aatcgacgct ccacgcgagc cgctcgcgga gcccggctcc gttttctggc
    13261 gcaactggca gcgctggcgg agcggggatc tcgaactata cctctatctc ttcgaggcgc
    13321 aacggcaggc gccccgcctc gagctgcaag cccggcggcg tccgctgctc gaggcgctcg
    13381 cgctcgagcg ccgcctgcaa gagtcggatc tgggccggtg gagcgaggcg ggaacgccgc
    13441 tcgatctgcg ccttgccaaa gagctggggg cgctctttcc aggcctctcc cgcctgctcg
    13501 caagccggca gcctgagcgg gaccagaaca cgctgcggcc cgcggtgatc gagctgcttg
    13561 aagcagcaaa caccgcatcc cccgagcgcc gggccatgtt actgctggcc gctgaccgcc
    13621 tcgccggccg cctggggcca accgagcgcg aatctcccca gtgggagcag gaacggaagc
    13681 agttggcggc ttacggcctt aacttcgagt gggacgagct cggcggctct tgggcctaca
    13741 cgcacgacct cttatggcgc gtttgggaag actatgccgg aatgccctgg ggcgagcagg
    13801 cgttcctgct gctcgaatcc cgcggatgga acacccatgt cggctgtcag gcgggcagtg
    13861 acgaatttcg catggtgatt cagaccggag agcggtttct gcgcgaatat accgaaagcc
    13921 agaaccggcc tgacgtactg ctgttcgttg ctcaaagcta tgagacctgg tggtcgctga
    13981 gccaggcagg cgagcgcgac gattatgttg accccgctaa atatcagcaa ggcgccgggg
    14041 cggcgagaga aagggcaatc cgactgtatg aacagctctt ggccaccgcc ccggacggcg
    14101 acggggccgc ccgcgcgcgc cagcaactgc cgcgtttgaa actcggcatc gacacggtgc
    14161 aacgccggtt cttttgcgtg tacgactgaa gaatatggtt gggcccgctc cgcggcgcac
    14221 tggccccgct tgcctggaaa tcagcgccct tgtgccgtgc gatagatcca gcggcgaacc
    14281 tccagccagc ggtcgggagg caagttgtct cccgccatca gccgggaaac gtcagcccgc
    14341 agtttgtcgc ggcccagttt tggcgcgagc gattcgagca gctccagatc ttgcaggacg
    14401 tcccgctcga tcctggactc cactgtcagc ggttcgttcc gagacgctgg aagcggcaaa
    14461 ggctgggcgt tccaccatgc agaaaagaat tggagagcga tcgtctgggc cggctgggca
    14521 gcgcgcgttt gatccggctg agttccagcg gccttagccc ctttccaatc ggaagctccc
    14581 ttccgatctg gaaaggcaat cgggaggatg cgcacctcca tcggatagac gacccgcggt
    14641 ccggcgcttt gcagcacgac ttccaaccga attctcccgg atggtaagtc gcggggggcg
    14701 tagagatcaa gccaataaat gcccgcggca tcggctctcg gccgcgccgg aatagaagcc
    14761 gggctggcgg gggctggctg gtgttcgggg gggtccggag cggactgctc atcgtcaggg
    14821 aaccgcccgg cgacggtctg cccgacaaga gtcagccggc ctgggtaacg ttcaccgctt
    14881 gcatcgggat cgccaaacca cacgcgatag agatcggcgc gcagccgtcc cacaggattc
    14941 atggcgatgt cgaggcgaaa gaagcctggc tcgcgcagtc ttgcggcgac aaaaaagccg
    15001 gcgtggccgt tgcggggcat ggctggcgag atgatctccc attggcgtcc gcccctgtcg
    15061 gcgggaacaa tatccccgcg cggggacggc cgctgaaact cgggatacag cagaatttct
    15121 tgcgccgcgg cgggccccag cgccagcagc agcagaacca gcacgaacat gcgaccatga
    15181 tactgcctgg ccggccttca gcagcggaat ccgggcatga tcatggcgtg gttacgctga
    15241 gcggctccag attggtaaac gcaccggagg gattaaagcg aagccccagc atggcaatac
    15301 ccccggcccc gcagccgatc aggggattga aggtgagatg gccgcgcttg ttccccattt
    15361 cgggaattct atccgtagcg atgaagccca tgtgaccgga gccatacata tttccgatca
    15421 attgcggctg tcccacgacg gctatgcccg attcatcgaa aataggaaac gcggcgacgg
    15481 tgcacgtcga aacagacaga ttcgcaatcg cgaacgtcgt gacgacgccg tttgtgttgt
    15541 cgaaggggaa cgtcatggag cttgccgcac gcattccgtt cgagacggct tcaaaatccg
    15601 gtcttccggg cacaatattg cggaaaatga caaaccccgc cacggcgccg ctcccggtgg
    15661 tctgatcgac ttcgacccac ccgacctgga cggccgggcg cgtgcccgca gtgtccagat
    15721 acagcgcgct ttgtacgccg aggtccgtat caagcgtgct gagcgaaccg tagttattca
    15781 aatccaccgg aacaagcgcc ccgtcatctc cgcgaaattt gatggtgagg tgcacgggag
    15841 tcacgttcac gccgttgttg aaaacggcga tggtgctctt ccagccgccg ccgtcggcga
    15901 agtgggggat tacaagaggc ggctgctgcg caaagctcag agccgtggtt gagaaagcta
    15961 atagagccag tatccgcatg gccggcgcct ccgtaaggat tggtctcggg tcccgactag
    16021 cgatcctagc agcacaccgg cgccgaacta aaatgctttt cttacctact accagcaggt
    16081 ggaacgcctg agagcggcgc ttcttgtttg cagggtgagc cggctcatcg ccgggcggat
    16141 cggagacgcg gtgagaagag ggcagacgat ccccgtctcg ccgccgtgcg tggcttctga
    16201 ttccaaagaa cttttccatc cccgcgccat gcgcaaaggt tttaagatgt tttcttcatc
    16261 cacgttgcta cagtaagaat cgcaatgaaa actttgctct ttttattcct tctccttgct
    16321 atcgccgccg cggccgcgcc ggcgaatatg ccgaatttgg cggaactcca gcgcatgatg
    16381 gcccgattcg ctcccgtgga gctgcgcgtg gatacttccg ccctttccgc taacgaccgc
    16441 caggccctgc tgaagatgat tcaggccgcg cgggtgatga acgatatttt tctaaaacag
    16501 atttggagcg gtaatcccga gctttacgcc agattgcaga aagatcatac gccgctgggc
    16561 cgcgcccggc tgcattactt ctggatcaac aagggcccct ggtcggagtt ggacggccac
    16621 agggcgttcc tgcccggagt cccgccgagg aagctgccgg gagccaattt ttatcccgaa
    16681 gacatgacca aagaggagtt cgaagcgtgg gtgaaagggc ttccgccggc cgggcgccag
    16741 gctgcggaag ggtttttcac ggtaattcgc cgcgattctt ccaaaaatct gaccatcgtt
    16801 ccctataaca aggaatatgc agccgacctg gagcgggccg ccgccttgct gcgcgatgca
    16861 gcgggcctga cgggcaacga gtctctcaaa aggtttctca acttgcgagc cggagcgttt
    16921 ctgtccaatg attactacgc cagcgatgcg gcctggatgg acctggatgc gcccctcgag
    16981 ccgacgatag gtccttatga gacttataat gacgaaacct tcggctacaa ggccgccttt
    17041 gaggccttta tcaatctgcg ggacgagaag gaaaccgggc ggctgcaatt cttcgccaag
    17101 catttgcaag aagtggaaga caatctgccg atcgagccgc gctatcgcaa tccgcacatc
    17161 ggatctctcg cgcccatccg agtggtcaac gagatttacg ccgccggcga tgcggcgcac
    17221 ggcgtctgca cggccgccta caatctgccg aatgacgagc gcctcgtcaa cgaaaaggga
    17281 agcaagcggg tcatgttgaa aaacgtccag gaggcgaaat tccgcaccac ccttgtgccc
    17341 atcgcaggca gggtgctcgc caaatccgcc caggcggatg tcaacttcga ctccttct
//
LOCUS       DASREK010000044         7274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_519335, whole genome shotgun sequence.
ACCESSION   DASREK010000044 DASREK010000000
VERSION     DASREK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519335"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFA54_02585"
     CDS             complement(<1..684)
                     /locus_tag="VFA54_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS49719.1"
                     /translation="MRPNRIKQLLKQGQTVLGCGLAYFRSPEVPRLLAAAGLDWTYID
                     TEHGCFNVETVQDFVRTALLSTLTPLVRVADLQYSLIARALDMGAEGIVLPRVESPDL
                     LARAISWTKFPGRGVRGYGLGPWQVNYENLAFQQIIDHVNENVLVVLQIETQTALDRC
                     DDLLAVPGVDIVMIGPADLSISLGVPGQFDHPKMIEAICGVIDRCRRHGIFPGIQCRT
                     LPMAKSWKQR"
     gene            789..1130
                     /locus_tag="VFA54_02590"
     CDS             789..1130
                     /locus_tag="VFA54_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZS49720.1"
                     /translation="MRNWVRLLSLGVFVLGWALAQESPRMKTVEPANGKAGDEITVSG
                     ENLDSKFVKKVYLTDGKQDIELEVTQQTAESIKVKIPSKAKPGRYSLMVLTADKDPKL
                     IEQPVKCTVEQ"
     gene            complement(1133..2287)
                     /locus_tag="VFA54_02595"
     CDS             complement(1133..2287)
                     /locus_tag="VFA54_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS49721.1"
                     /translation="MAPLLALVVPLAIASPIQQGWDHFYNLEYDQAIAAFERVIAASP
                     AAPENYNYLAEAILYKELYRLGALESELLTGNNYFLRNEKIQPDPQQNARFLAALEKG
                     RRLAEERLASNPNDADALYALGSSYGLRTNYDFSIQKAWRAAANDISLARKYHARVLQ
                     LAPGNYDAMLIQGVYDYAMGSLPWHVRLFSYLVGRSGDKNRGIRTIQLVSERGKLNRI
                     DAEVLLLVIYRRERQPEKAIALLNPLIRAYPRNYLFRMEKAHMYSDLGNKEAALAELR
                     ELEKLAAGGNPGYANIPIEKVYYAEGDVEFWYNDLEASLAKMTRVTEYPDLDLHTGVL
                     AWMRQGQLLDLMGRRKEAVQAYRKAIRFAPQTAAAQESRRYLSSPYTRER"
     gene            complement(2348..2806)
                     /locus_tag="VFA54_02600"
     CDS             complement(2348..2806)
                     /locus_tag="VFA54_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZS49722.1"
                     /translation="MPIAVGQKAPDFTLKDQNQKDVKLSDFQGKNVVLAFYPLDWSPV
                     CSNEHACFTNDLKDFENLNAQVLGISVDSVWSHKAFAEKLGIRYPLLADFHPRGAVAS
                     QFGLYLADKGITNRATVIVDKHGVVRYVQVYDIPTQRSNADLVAELKKWN"
     gene            complement(2930..4006)
                     /gene="queG"
                     /locus_tag="VFA54_02605"
     CDS             complement(2930..4006)
                     /gene="queG"
                     /locus_tag="VFA54_02605"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZS49723.1"
                     /translation="MPALDLSAAKALHSGAVKALAQECGFDLAGITTAGPLREAGFYT
                     EWLARGYAAEMEYLYGRRAEMRGDVRTLLPSARSVICLGILYNTPVPYSTQWSEPDLA
                     WISRYAWGEDYHDLLRARMEELASRMQQLAAFEFKICVDTSPVLERALAQRAGLGWIG
                     HNTCLINQKIGSWVFLGEILVSLELEPDEPAPFRCGSCRKCIEACPTGAIVPTGKLEG
                     PAYALDSRLCISYWTIEHRGPMPGGHRGQVKNNVFGCDICQDVCPWNRRAPATENPAF
                     APRWNNPPLDKFAAITEEEFRQIFRSSPVQRARYSGFLRNVAVAMGNSGNPRYREALE
                     NLAASGGELVREHALWALQKIGGL"
     gene            4198..6015
                     /locus_tag="VFA54_02610"
     CDS             4198..6015
                     /locus_tag="VFA54_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HZS49724.1"
                     /translation="MKPPVRKWTFAFAGLAALILLAPALGAADATPTGNAYLQHNLVA
                     DTPGIADHTDPNLIDAWGISESAASPFWVSDHGAGLSTLYNSTGVPQALKVIIPAGAA
                     SGSGAAGKPTGQVQNSAAASGAFAVATGRTANFIFATEDGTISGWNANVDATHAIVKV
                     DNSTSGAVYKGLALGTPASGARLYAANFSAGTIDVFDANFNPVTPASGAFTDAQIPAG
                     FAPFNIQNLGGKLYVTYAKQDSDKVHDVAGLGNGYVDVFDLNGTLLQRLAAGGPLNSP
                     WGVAIAPANFGPFSNDVLVGNFGDGKINAFDPATGAFLGALQDPAGNVISIPGLWGLQ
                     PGNGGNGGDKNAVYFAAGPGGEKHGLFGSLQASPVITASGIVNSGSSLPVIAQNTWIT
                     IFGANLAATNRAWASADFSGNKLPTSLDGVSVMINGKPAYVGFISPSQLNVLTPLDAT
                     LGPVPVQVTNNGLTSAAASVSLQPTAPAFFMYKNNYIAAVHSDNVSIVGPAALFPNAS
                     TPAKPGETIVMYGTGFGPTNPPIPGGTIVSSANPLISTPIVMFAGIGAQVIFGGLTGA
                     GLYQFNVVVPPSAPDGDLEVQAVIGGVETPPGTFINVKR"
     gene            6090..6674
                     /locus_tag="VFA54_02615"
     CDS             6090..6674
                     /locus_tag="VFA54_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZS49725.1"
                     /translation="MTAPSQGLAGRTRPVFTVGHSTLPLESFTGRLQAHGVQRVVDVR
                     TIPRSRRNPQFNSGALAASLEAVHIGYTHMPELGGLRRPQKDSRNTGWRNASFRGYAD
                     YMQTPVFEKHLLRLIELSEKEQIAVMCAEAVPWRCHRTMIADALTAHQIPVEHILSDT
                     VRRPHTPTPFARLEGVRVTYPAGEPQQVSLLEGL"
     gene            6786..>7274
                     /locus_tag="VFA54_02620"
     CDS             6786..>7274
                     /locus_tag="VFA54_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZS49726.1"
                     /translation="MYTVRVPASSANLGPGFDALAIALGLYLECRFRRSDRLRIHASG
                     RDAGLISEDETNLIWRVAVAAARQAGAEMPPLEIEIRNEIPVGKGLGSSAAALIAALA
                     AADCVLKLGWTRERILDEAARLEGHPDNAAACVLGSMVVSATGSDGKTRAVRLAMANG
                     FRV"
BASE COUNT         1477 a         2182 c         2187 g         1428 t
ORIGIN      
        1 gcgctgcttc caggacttag ccattggcag cgtccggcac tgaatcccgg gaaaaatgcc
       61 gtgccgccgg cagcggtcga tgactccgca gatggcctca atcattttgg ggtggtcgaa
      121 ctggcccgga acgcccagtg aaatgctgag atcagccggc ccgatcatca cgatgtccac
      181 gccgggtacg gcaagcaaat cgtcgcaccg gtccaaagcc gtttgcgttt cgatctgcaa
      241 aaccacgagc acgttttcgt tcacatggtc gatgatctgc tgaaaggcga gattttcgta
      301 attcacctgc cagggcccca ggccgtagcc gcgcacgccc cggccgggaa acttggtcca
      361 actgattgcc cgggccagta gatcgggaga ctcaactcgg ggcagcacaa tgccttcggc
      421 tcccatatcg agggccctag cgatcaggga atattgcagg tcggccaccc gcaccagcgg
      481 ggtcaatgtg ctgagcagag cggtacggac aaaatcctgc accgtttcga cgttgaagca
      541 gccatgctcg gtatcgatgt aggtccagtc aagcccggcg gcggccaaaa gcctcgggac
      601 ttccggggaa cggaaatacg ccaggccgca acccagcacg gtctgaccct gcttgagaag
      661 ttgcttgatc cggtttggtc tcatttttac gaggaagacc tgaagtttaa catacttgca
      721 tttctacaca tggcggcata acatggcgat atccagcacc cccaaacgaa atgggaagga
      781 ggccagcaat gaggaattgg gtgcgcctgc tatcactagg cgttttcgta ttaggctggg
      841 cgctcgctca agaatcgccg cggatgaaga ccgtggagcc ggccaacggc aaggcaggcg
      901 acgaaatcac cgtaagcggg gaaaacctcg actccaaatt cgtgaaaaaa gtttacttga
      961 ccgacggaaa acaagatatc gagctcgagg tcacgcagca gacggccgaa tcaatcaaag
     1021 tcaaaattcc atccaaggcg aagcccggcc gctacagcct gatggtgctg accgctgata
     1081 aggatccgaa gctgatcgag cagccggtca agtgcacggt cgagcaatag ctttaccgct
     1141 cccgcgtgta gggcgaactc agatagcgtc tggactcctg cgccgcggcc gtttgcggcg
     1201 caaagcgaat ggctttgcgg taagcttgca cggcttcttt tcgtctgccc ataaggtcca
     1261 gcaattggcc ttggcgcatc cacgccagaa cccccgtgtg gaggtcgagg tcggggtact
     1321 ccgtcacccg cgtcatcttt gctaaagaag cctccaggtc gttgtaccaa aactccacat
     1381 cgccttcggc gtaatagacc ttttcgatcg ggatgttcgc atagccgggg ttcccgccgg
     1441 cagccagttt ttcgagctcc cgcagttccg caagcgccgc ttctttgttg ccgagatcgc
     1501 tgtacatatg ggctttttcc atgcgaaaga gataattgcg gggataagct cgaatcagtg
     1561 ggtttaacag cgcaatggct ttctccggct gcctttcgcg ccggtagatc accagcagca
     1621 ggacctcagc gtcgatgcgg ttcagcttcc cgcgttcgga tacgagctgg attgtccgaa
     1681 tgccgcggtt tttatcgccg gagcgcccga ccagataact gaacagccgg acatgccagg
     1741 gcaagctgcc catcgcgtag tcgtagacgc cctggataag catcgcatca taattgccgg
     1801 gcgcaagctg gagtacgcgg gcatggtact tccgggcaag agaaatatca ttagccgccg
     1861 cccgccaggc cttctggatc gagaaatcat agttcgtccg caagccgtag gaagatccca
     1921 gagcgtacag ggcgtctgcg tcgttgggat ttgacgccag gcgctcctcc gccaatcgcc
     1981 ggcctttttc gagcgccgca aggaaacggg cgttttgctg gggatccggc tggatctttt
     2041 cgttgcgaag gaagtagtta ttgccggtaa gcagctcgct ctcgagcgcg cccagccggt
     2101 aaagctcttt gtagagaatg gcctcggcca agtagttgta gttttccggc gccgccggcg
     2161 acgccgcaat cacccgctca aaagccgcaa tcgcttggtc gtattccaga ttgtaaaagt
     2221 gatcccagcc ttgctggatc ggactggcga tagcaagcgg aacaacgagc gcgagaaggg
     2281 gcgccatgag cacgtgatag ggagctggtg cgcggggccg ggcttgccaa accggcgctg
     2341 gcccgcgcta attccacttt ttgagttcag ccaccaggtc cgcgttgctg cgctgcgtcg
     2401 ggatatcgta aacctggacg tagcgcacga cgccgtgttt gtccacgatc acggtagcgc
     2461 ggtttgtaat gcctttgtcg gccaaataga gcccgaattg agacgcgacc gcgccgcgag
     2521 gatgaaaatc ggcgagcagg gggtatcgga tgcccagctt ttcggcaaaa gccttgtggg
     2581 accagacgct gtccacactg atgcctaaaa cctgggcgtt caagttttcg aaatctttca
     2641 aatcgttcgt gaagcaggcg tgctcgttgc tgcacacggg actccagtcg agcggataaa
     2701 acgcgagcac gacgttcttg ccctgaaaat cactgagttt gacatccttc tggttctggt
     2761 ctttgagcgt aaagtccggg gccttctgac ccacagcaat cggcattctt gcgtctcctg
     2821 ttttttttcg tgaaatgacg gccaaaccaa actaaagtaa cacaaccgcg ggaaatgaag
     2881 ccctggatgc ggccggcaaa cagagcagaa gcttcaactc cgaccggatt caaagcccgc
     2941 cgatcttctg caaagcccac aaggcgtgct ctcgaacgag ctcgccgccg gatgccgcca
     3001 gattctccag agcctcgcga taccggggat tgccgctgtt tcccatggcg acagcgacgt
     3061 tgcgaagaaa tccggaatac cgggcgcgct gcaccgggct cgagcggaaa atctgacgga
     3121 attcttcctc tgtaatcgcc gcgaacttgt cgagcggcgg gttgttccag cgcggcgcaa
     3181 aggccgggtt ttcggtggcc ggcgctcggc ggttccaagg gcatacgtcc tggcagatgt
     3241 cgcagccgaa tacattattt ttcacctggc ccctgtggcc gcccggcata gggccccggt
     3301 gctcgatggt ccagtaggag atgcagagac gggaatccag cgcgtaggct gggccttcga
     3361 gtttacccgt agggacaatc gcgcccgtag ggcacgcttc gatgcacttc cggcaggacc
     3421 cgcagcggaa tggggccggc tcatcgggct caagctcgag cgaaaccaaa atttccccga
     3481 gaaacaccca agagccgatc ttctggttaa tcagacatgt gttgtgcccg atccagccga
     3541 gacccgcccg ctgcgcgagg gcgcgctcga gcacaggcga tgtgtctacg cagattttaa
     3601 attcaaacgc cgccaactgt tgcatccggc tggcaagctc ttccattctc gcccggagaa
     3661 gatcgtggta gtcttctccc cacgcatagc gggaaatcca agccaaatcc ggctcgctcc
     3721 attgcgtcga atagggaacc ggcgtgttgt agagaattcc caggcaaatg accgagcgag
     3781 ccgacggcag cagcgtccga acatcgcccc gcatttcggc gcggcgccca tataggtatt
     3841 ccatttcggc ggcatacccg cgggcaagcc attccgtata gaagcccgct tcgcggaggg
     3901 ggccggcggt tgtaataccc gccaaatcaa agccgcactc ctgcgcgagc gccttcacgg
     3961 ctccggaatg cagcgctttt gcggcgctca aatccagcgc gggcatgtag gtctcttcga
     4021 aagcaatggc cgccttcggc ggcttgggct ctgttgttca atcaaccgcc gagccgcgac
     4081 tgcggcttct tcgcgaccca accgccggac atgccggtag gcacaaacgt tacaattctt
     4141 aatcaaacag ccccggtata atcgtcctgc caattgaaaa atcaggagga tcctgcgatg
     4201 aaacccccgg tgcgcaagtg gacttttgct tttgcgggac tcgcggctct tattctgctg
     4261 gcgccggcgt tgggcgcggc cgatgccacg cctaccggca atgcctatct gcaacacaat
     4321 ctcgtggccg atacgcccgg gatcgcggac cacacagatc cgaatctgat cgatgcctgg
     4381 ggcatcagcg agtccgccgc cagtcccttc tgggtgtccg accacggcgc cggcctttcc
     4441 actttgtaca atagcaccgg cgtgccgcaa gcgctcaagg tcatcatacc ggcgggagcg
     4501 gccagcgggt ctggcgccgc cggcaaaccc accgggcaag tgcaaaacag cgccgccgcg
     4561 agcggagctt ttgccgtagc taccgggaga accgccaact tcatcttcgc gacagaggac
     4621 gggaccattt ccggctggaa cgcgaatgtc gacgccacgc atgccatcgt gaaagttgac
     4681 aactccacat ccggggcggt ttacaaaggc ttggctctgg ggacgccggc ttctggcgcg
     4741 cggctgtatg cggctaactt cagcgcggga accatcgacg tcttcgatgc caatttcaat
     4801 cctgtcaccc cggcctcggg ggccttcacc gatgcccaaa tcccggcagg cttcgcgccg
     4861 ttcaatattc agaatctggg cggcaaactg tacgttactt acgcgaagca ggattcggat
     4921 aaagtacacg acgttgccgg cctgggcaac ggctacgtgg atgtgttcga cctcaatgga
     4981 accttgctcc agcggctggc cgccggaggc ccgcttaact caccgtgggg agtggcgatc
     5041 gcgcccgcca acttcggccc gttcagcaac gacgtgctgg tggggaattt cggcgacggc
     5101 aaaatcaacg catttgatcc cgccaccggc gccttcctgg gagcactgca agatccggca
     5161 ggcaatgtga tctcgattcc gggactttgg ggcctccagc ccggcaacgg cggcaacggc
     5221 ggcgacaaga acgctgttta tttcgcggcc ggtcccggag gagagaagca cggcctgttc
     5281 ggcagccttc aagccagccc ggtgatcacc gcgagcggga tcgtcaattc gggcagctcc
     5341 ttgccggtga ttgcccagaa cacctggatc acgatcttcg gagccaatct cgctgcaacg
     5401 aatcgggctt gggcgagcgc cgatttttca ggcaacaaac tgcccacgtc ccttgacggc
     5461 gtcagcgtca tgatcaacgg aaagccggct tacgttggtt tcatcagccc gagccagctc
     5521 aacgtcctca cgccgctcga tgccacgctc ggtcctgtcc cggtgcaggt gaccaataat
     5581 ggccttacca gcgcggcggc gtcagtttcc ttgcagccga ccgcgccggc gttcttcatg
     5641 tacaagaaca attacattgc ggcggtccat tccgataatg tttcgatcgt tgggcccgca
     5701 gcgctgttcc ccaatgcgtc tacgccggcc aagcccggcg aaacgattgt gatgtacgga
     5761 accgggttcg ggccgacaaa tcccccgatt ccaggaggaa cgatcgttag cagcgccaat
     5821 ccgctgatct cgactccgat cgttatgttt gccgggatcg gggcgcaggt gatcttcggg
     5881 ggcctcaccg gcgcggggtt gtaccagttc aatgtggttg tgccgccctc ggcgccggac
     5941 ggcgatctcg aggtacaagc agtcatcggc ggagtggaga cgcctcctgg tacgttcatc
     6001 aacgtgaaac gctgaaacgt tccgtaccga acgccggcag gtaagattca accaggatgc
     6061 gcgctggggc tatccttgag ataaccctta tgaccgcgcc ctcccaaggc ctagccggca
     6121 gaactcggcc ggtcttcacc gttgggcatt ctacgctgcc tctagaatcg ttcacgggcc
     6181 gtctccaggc gcatggggtg caacgggtgg tcgatgtccg gacgatccct cgttcgcgcc
     6241 gcaatcccca gttcaattcc ggcgctttgg ccgcttccct ggaagcagtg cacatcgggt
     6301 acacgcacat gcccgaactc ggaggtttgc gccggcccca aaaagattcg aggaataccg
     6361 gttggcgcaa tgcatccttc cgggggtacg cggattatat gcagaccccg gttttcgaaa
     6421 aacacctgct gcggctgatc gagttgtccg aaaaagagca gatcgctgta atgtgcgccg
     6481 aggccgtccc ctggaggtgc caccggacaa tgattgcgga tgccctgacc gcccatcaaa
     6541 ttccggtgga gcacattctg agcgacacgg ttcggcggcc gcatactccg actccttttg
     6601 cgcggctgga aggggttcgg gtgacatatc cggccgggga gccgcaacaa gtctcgctgc
     6661 tcgagggcct ctgaggatgg aaaagagcgg ccggcttcgg gcggctgcga agccggccgt
     6721 ggcggcgaaa gaatcggcct gcgggccagc tttggcggcg gcctgcaagc tataatgagc
     6781 ggcttatgta cacggtccgc gttcctgcct ccagcgctaa cctggggccc ggatttgacg
     6841 ctttggcgat cgctttgggc ctctatttgg agtgccggtt ccgccgatcc gaccggctgc
     6901 gcattcatgc ctccggccgc gacgcgggct tgatttccga agacgaaacc aatttgattt
     6961 ggcgggtggc ggtggcggcc gcccgccaag ccggcgccga gatgccgccg cttgagatcg
     7021 aaatccgcaa tgaaattccg gtgggcaagg ggcttggatc gagcgcggca gccctgatcg
     7081 ctgcgctggc ggcggccgat tgcgttttga aactgggctg gacgcgcgag cggattctgg
     7141 acgaggcggc gcgcctcgaa ggccatccgg ataacgcggc ggcttgcgtg ctgggatcta
     7201 tggttgtgag cgcgactgga tcggacggta aaacccgcgc cgtgcggctg gcgatggcca
     7261 acggatttcg cgtg
//
LOCUS       DASREK010000045        18431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1180730, whole genome shotgun sequence.
ACCESSION   DASREK010000045 DASREK010000000
VERSION     DASREK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 18431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18431
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180730"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFA54_02625"
     CDS             <1..530
                     /locus_tag="VFA54_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49727.1"
                     /translation="HQNRGIGRELKLAQRKEALERGIRLIEWTFDPLEIKNAHFNIER
                     LGAVVRRFVPNQYGVSSSPLHGGLPTDRCVAEWWIDSPRVRAIVEGRRALFTAEARIE
                     VPREIDAWKRENPARAREVQSGIREQFQMWLARGLAVLGYEIGPRAGAFLFGKWEGGE
                     GPLNPEPGRAGPSRS"
     gene            567..1676
                     /gene="menC"
                     /locus_tag="VFA54_02630"
     CDS             567..1676
                     /gene="menC"
                     /locus_tag="VFA54_02630"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HZS49728.1"
                     /translation="MKITRITLREIHLPLVHFFETSFGRTTQRRIVLVEVEEAGGAAG
                     WGEVTAGENPFYNEEWTESAWLILRDYAGPRVLRRDLRNACEAPELTARIRGHQMARG
                     GLEAAAWDLEARLAGRPLWRHIGGVRAEIACGVSIGIQDSVEQLLQKIQCELEAGYQR
                     IKIKIKPGWDVDVVRAVRERFPDIKLMADANSAYRLSDAPHLKQLDAFGLMMIEQPLA
                     HDDIIDHAKLQARLDTPICLDECIRNAHMARQAIEMRACGIINIKLGRVGGFAEARRV
                     HDVCREHGIPVWCGGMLESGVGRAHNIALSTLENFVLPGDVSASRRYWQRDIITPPVE
                     VTPQGTIQASERPGFGYELDLDYIRSVTAREEILR"
     gene            1673..2605
                     /locus_tag="VFA54_02635"
     CDS             1673..2605
                     /locus_tag="VFA54_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZS49729.1"
                     /translation="MIAGSSVEAIAGSGRLPPRPGRLYSLVGLMTFFWSANFLIGKIA
                     LREFPGPLAAALRILFAALASVPLCRWKTGNWGRWQKRDGFILASLGAVGIGVNQFCF
                     LVGLSKTSIAHSALIIGTTPISVLLIAAARGMERITGLRLLGMLAALGGVALLGREHG
                     AAAAGGAIPTLAGDLITFLASAAFAAYIVFSKEVRRRYAALPANAWLFGSAGVCVLPM
                     ALWQGWRFPFSHVSAAGWLAILYMAAFPSLFCYLVFYWALDYFAASRLSAFSYLQPVL
                     ATLLGVLLLGERISAPLLAGGAVILGGVFLTERG"
     gene            2605..3897
                     /locus_tag="VFA54_02640"
     CDS             2605..3897
                     /locus_tag="VFA54_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS49730.1"
                     /translation="MLSFRQLLRQNRNYRLIWMGQVVSEIGDHFNNIAVFSLALESTR
                     SPMVVSGIMISRAIPAILAGPLAGVLLDRADRKRVMIASDVVRAVMALGFLLYHPGGS
                     SWLLYLFSGLLMLASPFFTSGRSSILPTITNAEELHTANALTQTTQWATLTAGTLLAG
                     GIVTRFGFSRAFLFNAFSFVISATAISLLRLEKGEFRARRALTEADVVRPWREYVEGL
                     RYMRSVPLVFGISLIGVGWATGGGAAQILFSVFGELVFQRGPAGIGMIWGFAGIGLLI
                     GGFWAHQMAKRLTFEGYKKTILVCFLLHGAFYVVFSQMRVFTYALLAILFSRLVIAIS
                     SVLNFSRLLHTVPDQFRGRVFATVETMVWSTMMISMMLAGIGSQYYSPRTIGTIAGVL
                     SSSTGVFWAWAHFTGRLPEPAEQGVADEEVEIHGEAVV"
     gene            3860..4687
                     /locus_tag="VFA54_02645"
     CDS             3860..4687
                     /locus_tag="VFA54_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HZS49731.1"
                     /translation="MKKWKSTARRSFEPLRPAAARVSPVRRGLWGALLLLLAWAGAAR
                     ADVPPLQPKGYTNDYAGVLSAGAVAQADALAAEVERKTGAQMAVVIVKSLDGEAIEDY
                     ANTLARRWGIGKKDNRGILLLLAIQERRDRLEVGYGLEPILPDGKVGGILRGLRLYLQ
                     QGNYDAAVLTALGEISAVIAQEAGVTLETHALPRPPAAQYHSRGVDLPWWVILLGVGG
                     LVLFILITGMNPLFFLMGGMPGWGGPSGRERDGNWDGGGFSGFGGGDFGGGGASSDW"
     gene            4807..5511
                     /locus_tag="VFA54_02650"
     CDS             4807..5511
                     /locus_tag="VFA54_02650"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZS49732.1"
                     /translation="MEDLLTDLVERLQRAYGGELLSVVLYGSAATSDYHEQHSDLNVL
                     CVLERVGLPELERSGEALRWWTKQGQPTPLLLSAEEVRDSTDVFPIEFLDIKESYRVL
                     YGADLVKDIQVDMSKHRTQLEHELRSRLLRLRKHFLKTQHDDKAVSLLMLESLPTFAT
                     LFRHVLLAAGFPAPVKKHDIFQAAAQHLHVGPSPFDALLEVRKGSRKLVAGELRSWFE
                     SYLAAITAVSEIVDKL"
     gene            complement(5532..6122)
                     /locus_tag="VFA54_02655"
     CDS             complement(5532..6122)
                     /locus_tag="VFA54_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49733.1"
                     /translation="MTKVILLAAAAGVLSASSVVHLGFTDLSRLARRVVVGRIERITS
                     YQDTASGRIHSRVEIAESRSIAGAAPAAFTFEMIGGAVGDLRQWLADFPQLQEGDRVV
                     LFLAEDTNTPFGPTIGLWQGVFFIDRDPLTGVDVIADHRRRPLAEIRGDQLITVETPA
                     AQSGSAGPRRAGSAARLTLDAFLSRVSTLRAEKTAH"
     gene            6177..8750
                     /locus_tag="VFA54_02660"
     CDS             6177..8750
                     /locus_tag="VFA54_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HZS49734.1"
                     /translation="MKFTECFGTSAPFSRYTVGRRALHRYFHMLVLFFAGAAACLAAG
                     SGTTADDTACMQGGFKLITFGNTPVIGKAGASFNVFFDSAGFEGNSFDSALRSAMSSW
                     SSVSASAWRYNFSGYTAAGASPNDQQMEIIKGGMSFPSGVLATTLVGALVSTGQITDS
                     DTFFNPAYSLSTSPGPNDFDFQSVALHEMGHGLGLAHNDGCNPQPTVMQSAIAPGEQR
                     RNLFSPELAGVTYLYPGSGGGGLGVSPSSLIFTGAAGGASPAPQTLLVSAPQGAAWSA
                     AVATSSGGNWLSVSPQSGAGPATLTVNVAAASLDAGFYAGQITVSGGGSSSAVSVSLN
                     LAGIGASPSSLTFTATVGGAQPPPQGINLSGAPGAAWTAATSTADGGNWLSISSSAGY
                     LPATVLVSVSTAGLAASAYTGSITISSGGASGQVSVQLAVSSEAPLVVSPAQLSLSGA
                     AGSAAPVCAPASIRGSGSASLDWSASLGASWLSIQPASGRTPASISICATPGSLPAGN
                     YSANVSITSAAPNSPVNIAVSFSVTASVTVSSGGVVNGASFTGGQPIAAGEILSIFGA
                     NLASQTASAAGFPLPTALADCRVQIAGVPAPLLYVSPGQINLVAPTALSNLSGSSTTL
                     VVFNGLLASQPIRVSVAGQAPGVFSVLGNAAGAGAVTHQDGSLVSRSAPLVPGEPISV
                     YLTGMGPLSPPVPDGAPAPVNPLARALNNVQLILDGQPVQPLYAGAAPNFAGLQEVVA
                     LVPASLSHRFPQMSVIVQGAASNPFSAGGPTLYDVSPASVPPGADATVTLRGINLPST
                     SAVLAGGAPLPATLSQSDWQTLRVRIPARLLASGSLSLAVMDTAAPSEPPSNSISLSV
                     Q"
     gene            8822..9682
                     /locus_tag="VFA54_02665"
     CDS             8822..9682
                     /locus_tag="VFA54_02665"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HZS49735.1"
                     /translation="MGSGKPAAAGGELPYTVLRLESWDEFLQLITDSPYSNWAFRGLA
                     DERWPLSSKLSRYFENFRVDRRAWPEQEERILRIFKRKAHHFLAQPPDPDDEFQWLAL
                     MQHHGAPTRLVDFTWSPYVAAFFALEAATGDAAVWALNPVPLSYVHEQTIQGGRRIAP
                     PDMNPRVEGNLRKYFLKADIPFIWVGEPQVMNRRLIAQSGTFAIPGVLTQPMEEILSS
                     YPDPQHTLVKFVLPAGKVRKRGLRELYRMNITNATLFPDLDGLARSLAYELEFHWRYD
                     PQTMERYLDY"
     gene            9918..10706
                     /locus_tag="VFA54_02670"
     CDS             9918..10706
                     /locus_tag="VFA54_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZS49736.1"
                     /translation="MQRLGCILLCSLLALPALAQSSSVFIEDLTWTEVRDAIAAGKTT
                     AIYYAGSIEQNGPGMALGKHLFLAHYIAGQIAEQLGDALVYPTMPFAPVGDPLKKTGH
                     MRFPGSTSVSEATFEGVARDVALSAISAGFKNVVMMCDHGGGQNGLKKVAAEMNQEWG
                     PKGVHIYYIPDLYYKEKEQMRDYLTKHKMRIDQHAGVDDTSEVMFIDREHKWIRKDKL
                     APDDGKMGVNGDPTQATPSLGKMFIDFKIQDAAAQIRSLMKSKP"
     gene            10796..11842
                     /locus_tag="VFA54_02675"
     CDS             10796..11842
                     /locus_tag="VFA54_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZS49737.1"
                     /translation="MSLRDFLAKQFIDVIQWVESEDGVLAYRFPMRDMEIQNGGKLTV
                     RESQMAAFVNEGQVADVFGPGLYTLNTHTLPLLTNLMNWDKAFQSPFKSDVYFFSTRL
                     QTNQRWGTATPVTIRDKEFGAVRLRAYGIYSYRLSEPRVFYTKVSGTRDIYHVADLEG
                     QLRNTIIGVMSDTFAQSTIPFLDMAANQLELGQKIHGRLQPVFADLGLALDSFVVENL
                     SLPDELQKRLDERIGMTMVGDMGRYTQFQVAQSMPIAAANEGAGGAAGIGAGLGAGLA
                     MGQAMFHAARQPAAEASPASPAPAGPEPGPATSGGGAAPGPAPGAETKFCLDCGKPIP
                     RRAKFCPECGAAQQ"
     gene            11847..12191
                     /locus_tag="VFA54_02680"
     CDS             11847..12191
                     /locus_tag="VFA54_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HZS49738.1"
                     /translation="MSGREWVVEAHGCDARALADLAKLQQLFRRLTGELALHAAGEPL
                     WHQFPYTGGITGLCLLTESHLACHTFPEYRSLCLNLFCCRPRPDWDFESYFKTEFAAS
                     SVRVRRIERPYE"
     gene            12188..12826
                     /locus_tag="VFA54_02685"
     CDS             12188..12826
                     /locus_tag="VFA54_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HZS49739.1"
                     /translation="MSSPSANCPNCGAPIRFRWSSAVQTTCDFCHSILVRRDVDLAKV
                     GEVADLPPDSSPIQIATEGVYRNRPFVVVGRIVYEYDAGSWNEWHIVFSDSSSGWLSD
                     AQDEYAVSFQTAPPQPLPPPEKVERGQFALWNGVRYQVTSLTRARYLGVEGEMPFENW
                     DKREALFVDLRSADGRFATIDYSEQKPLLFLGEAVEFDDLRLKNLRQFEGWS"
     gene            12826..14160
                     /locus_tag="VFA54_02690"
     CDS             12826..14160
                     /locus_tag="VFA54_02690"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HZS49740.1"
                     /translation="MPAEPPATGPSGSPPGSVSSVKALNCPNCGAALTLRAFQHTLSV
                     VCAQCLSVLDAKDPNLQILQQFEDRQRVVALIPLGSRGKWRNDLYEVIGFQVRTITDA
                     GAQYSWHEYLLFNPYKGFRYLTVYDGHWNDVKPVSAVPEPASSRGKSAVRLLGRTYVH
                     FQTASAETTFVMGEFPWRVKVGELVACTDYIAPPYILSSETVETETTWSLGEYVDGAR
                     VWEAFQLPGRPPRPAGVFANQPSPYAGKLKQLWFLCGILLLLLANLALLFSGFARKEQ
                     VFDHQYTFSPGARDASFVTPSFDLAGRTSDVELSIRTNLRSNWAYFNFALINEATGQA
                     YDFGREVSNYPGEGSPNDDVLIPSVPPGRYYLRVEPEMSSSSSSTRNSRMTYELILRR
                     DVPSPAFYWICVLLIVAPPVFLTLRAAGFEKSRWQEGGDITAESDSEPGEDS"
     gene            14160..14345
                     /locus_tag="VFA54_02695"
     CDS             14160..14345
                     /locus_tag="VFA54_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49741.1"
                     /translation="MGRFLYLAYGLALLGLTGAAEYRGWSLLSVNEVKNVPRTVRDNP
                     GAYRSFYHGSSRYVGGK"
     gene            14345..14557
                     /locus_tag="VFA54_02700"
     CDS             14345..14557
                     /locus_tag="VFA54_02700"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HZS49742.1"
                     /translation="MSEFNAGYVLNALVYSVLGIVIFVAAFAIVDRMTPYHLWNEIVH
                     EHNTALAILIGAMSIGMCIIIAAAVH"
     gene            14527..16173
                     /locus_tag="VFA54_02705"
     CDS             14527..16173
                     /locus_tag="VFA54_02705"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HZS49743.1"
                     /translation="MHHHRRRSTLSLSPRPGIRWESCLNAHEADGAFRCPANAGAACQ
                     RLKRFPMPVVLFISVLLIAACGLIYELIAGTLASYLLGDSVFQFSTVIGSYLFAMGLG
                     SGLSRYFTRGLVARFVSIELMVGLAGGFSSSILFLAFAYTPGFRLILYLVVILIGVLV
                     GLEIPLLMRILKGRFLFRDLVAHVLTFDYLGALGASLLFPILLVPKLGLVRSALLFGV
                     MNAAVALWSTFLFRDQLGSPHALRAMCGVVLLALCLGMLGAERITAAADTSLYADEVI
                     FSRQTRYQRIVLTKWKDDIRLFLSSHLQFSSRDEYRYHEALVHPGLAAIPAPRRVLVL
                     GGGDGLALREILKYANVERVTLVDLDPEMTRLFSENPFLAQLNGESLRSSRVQVINAD
                     AFPWLESTRDMFDFITVDFPDPTSYSLGKLYTTAFYRLMARHLSAQGMAVVQSTSPLF
                     ARQSYWCIVETLKQAGLRTYPYHVYVPSFGEWGFVLASQRPYQPPSQLPEGLRFLSAQ
                     NLGSLFEFPKDMLPVPAEPNRLNNQVLVRYYEQDWSDIAR"
     gene            16163..17746
                     /locus_tag="VFA54_02710"
     CDS             16163..17746
                     /locus_tag="VFA54_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZS49744.1"
                     /translation="MPVDRPLGERTPPLRGGGAAGGPVSRRQLLGAALIGLTPKGRRA
                     IAGSFVNEAHLLGHKLRDHGAFRKPSQTIQIPLVIVGGGVAGLSAAWRLHKRGFHDFV
                     LLEMEKQAGGNSRWGENEITAYPWAAHYVPVPGKRAALVRELFAELGVLKDGKWEERD
                     LCFSPQERLFQYGRWQEGIEPTAGLSSSEREQFRRFEARIEEFRATGEFTIPMEAGAG
                     SPRARTLHLDRLSMSSWLREEGFDSPHLNWYINYATRDDYGAPASDVSAWAGVHYFAA
                     REPEEKGPLTWPEGNGWIVKQLLARLGAFVRTDSPVYRIERLGSRWRALTEKAEYIAG
                     AVIFAAPTFLASYIVEGAAPAKGFVYSPWLTANLTLERLPREQQSEPAWDNVIYDSPG
                     LGYVVATHQTLRTHVERTVWTYYYAMAQAPPAENRRLLLAKDWGYWKEFILDDLERAH
                     KDIRSCVSRIDIMRLGHAMARPVAGFLTSEERRRAAGRSGSFLFANSDLSGFSIFEEA
                     QYRGVTAADRALQHLGGRR"
     gene            complement(17804..>18431)
                     /locus_tag="VFA54_02715"
     CDS             complement(17804..>18431)
                     /locus_tag="VFA54_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZS49745.1"
                     /translation="IACIIAHAFTVLVLRRSILTEKVSRRGYHLSCEYAVDPLEILFV
                     REVMRTNIAALPADVKLDDLATSLRTSGGRGGQRLYPVVDKDHHLVGVVTRHNIQKAL
                     RQSSDGPGARMADLVNPSPVVAYPDEPLRLVVYRMAETGLTRFPVVERGGHGKLVGMI
                     ALKDLLQARVRNLQEERRRERVLRTRIPFVSRPRRGTEMDSISSASRM"
BASE COUNT         3109 a         5788 c         5893 g         3641 t
ORIGIN      
        1 gccatcagaa ccggggcatc gggcgggagc taaagctggc gcagcggaaa gaagcgttgg
       61 agcgcgggat ccggctgatt gagtggacgt tcgatccgct cgaaatcaag aacgcccatt
      121 tcaacatcga gcggctcgga gccgtggtgc ggcgcttcgt tccgaaccag tacggcgtct
      181 cctcgagccc tctacacgga gggctgccca cggaccggtg cgtggccgag tggtggattg
      241 atagtccgcg cgtgcgggcg attgtggagg gccgccgggc cctgtttacg gcggaagcgc
      301 ggatcgaggt gccgcgcgag atcgatgcct ggaaacggga aaatccggcg cgggcccggg
      361 aagttcagag cggcattcgc gagcagtttc agatgtggct ggcgcgcggg ctggccgttc
      421 tcggttacga gataggcccg cgagccgggg cgttcctttt tggaaaatgg gaaggcggcg
      481 aggggccgtt gaatcctgaa ccggggcggg ccgggccctc ccggtcttga tggcgcgcgg
      541 ctgcgcgggc gatccatttc gggcttatga aaatcactcg catcacgctc cgcgaaattc
      601 atctacccct ggtccatttc ttcgagacca gcttcggccg cacgacccag cgccggattg
      661 ttctggtcga ggtagaggaa gccggcggcg ctgccggctg gggcgaagtg accgccggcg
      721 agaatccgtt ctacaacgag gagtggaccg aatcggcctg gctgattctg cgcgattatg
      781 ccgggccgag ggttctaaga cgcgatctcc gcaacgcctg cgaggcgccg gagcttacgg
      841 cgcgcatccg cggccatcag atggctcggg gaggtttaga ggcggctgcg tgggacctgg
      901 aggcgcgtct cgctggccgg cctctgtgga ggcatatcgg cggcgtgcgg gcggaaatcg
      961 cctgcggcgt ctcgatcggc attcaggatt ccgtcgaaca gttgctgcaa aagatccagt
     1021 gcgagctcga ggccgggtac cagcggatca agatcaagat caagcccggc tgggacgtgg
     1081 acgtagtgcg cgctgtgcgc gagcgttttc ccgatatcaa gctcatggcc gacgccaatt
     1141 cagcctaccg cttgagcgac gcgccgcatt taaagcagct cgacgccttc ggcctgatga
     1201 tgatcgagca gccgctcgcc cacgacgaca tcatcgacca cgccaagctg caagcgcgcc
     1261 tcgacacgcc gatatgcctg gacgagtgca tccgcaacgc gcacatggcc cggcaagcga
     1321 tcgagatgag agcctgcgga attatcaata tcaagctggg ccgcgtcggc ggcttcgccg
     1381 aagcgcgccg cgtgcacgac gtatgccgcg agcacggcat acccgtctgg tgcggcggca
     1441 tgctggagag cggcgtgggc cgggcgcaca acatcgccct ttccactctg gagaattttg
     1501 tgctccccgg cgacgtgtcg gccagccggc gctactggca gcgtgacatc atcaccccgc
     1561 ctgtggaggt cacgccgcag ggaaccatcc aggccagcga gcggcccggc ttcggctacg
     1621 agctcgatct ggattacatt cgcagcgtca cggcccggga ggaaatactc cgttgatcgc
     1681 cggaagctcc gtcgaggcca tcgcgggcag cggccggctg cccccgcgcc ccgggcgtct
     1741 ttacagcctg gtcggcctga tgaccttctt ctggtcggcc aacttcctga tcggcaagat
     1801 cgccctgcgg gagttcccgg ggccgctggc ggcggccctg cggatcctat tcgccgccct
     1861 ggccagcgtg ccgttgtgcc ggtggaagac cgggaactgg ggccgctggc agaagcggga
     1921 cggcttcatc ctggcgtcgc tgggcgccgt aggcattggc gtcaaccagt tttgcttcct
     1981 cgtaggtctt agcaagacca gcatagcgca ttcggcgctg atcatcggca cgacgccgat
     2041 ttccgttctg ctgattgcgg ctgcgcgggg gatggagcgg atcaccgggc tgcgccttct
     2101 gggcatgctg gctgcgttgg gcggcgtggc gttgctcggg cgggagcatg gggcggccgc
     2161 cgcgggcggc gccattccca cgctggccgg agatcttatc acgtttctcg ccagcgccgc
     2221 cttcgccgct tacatcgttt tcagcaaaga agtccggcgc cggtatgccg cgctccccgc
     2281 caacgcctgg ctgttcggct cggcgggcgt ttgcgtgctg cccatggcgc tgtggcaggg
     2341 ctggaggttt cctttttccc acgtgtcggc cgcggggtgg ctcgccatcc tgtatatggc
     2401 ggcgttcccg tcgctgttct gctatctggt tttctactgg gcgctcgact acttcgccgc
     2461 ttcgcgcctc tctgcgtttt cctatctaca gcccgtcctg gccaccctgc tgggcgtttt
     2521 gctgctgggc gagcgtatct cagcgccgct cctggcgggg ggagcggtga ttttgggcgg
     2581 cgtgtttctc accgagcgcg gctgatgctt tctttccggc agcttctccg gcaaaaccgc
     2641 aattaccgac tcatctggat gggacaggtg gtgagcgaga ttggagacca tttcaacaac
     2701 atcgccgtct tcagcctcgc cctcgagagc acgcgttcgc ccatggtggt gagcggcatc
     2761 atgatttccc gggccatccc ggcaatcctg gcggggcctc tggccggcgt gctgctggat
     2821 cgggccgatc gcaagcgcgt tatgatcgcg agcgatgtgg ttcgcgccgt gatggcgctc
     2881 gggttcctgc tctaccatcc cggcggcagc tcgtggctgc tgtatctgtt cagcggcctg
     2941 ctcatgctgg cttctccgtt tttcaccagc ggccggtcga gcattctgcc caccatcacc
     3001 aacgcggaag agctgcatac ggccaacgcc ctgacccaga cgacgcagtg ggccaccctc
     3061 accgccggga ccctgctggc cggcggcatc gtcacccgat tcggcttctc gcgggccttt
     3121 ctcttcaacg ccttctcctt cgtgatttcc gcgaccgcga tttccctttt gcggctggaa
     3181 aagggagaat tcagggcccg gcgcgccctc accgaggccg atgtcgtgcg tccctggcgc
     3241 gaatatgtcg agggcctgcg ctatatgagg tcggttcctc tggtcttcgg catttcgctg
     3301 ataggcgtcg ggtgggcgac cggcggcggc gcggcgcaaa ttctcttcag cgttttcggc
     3361 gagctcgtgt ttcagcgcgg cccggcgggc atcggaatga tctggggatt cgcgggaatc
     3421 ggcctgctca tcgggggctt ctgggcgcat caaatggcga agcgcctcac cttcgagggc
     3481 tacaagaaga ccattctggt ctgctttctg ctgcacgggg ctttttacgt ggttttcagc
     3541 cagatgcgcg ttttcaccta cgccctgctg gccatcctgt tttcccggct ggtgatcgcc
     3601 atcagctcgg tgctgaactt cagccggctg ctgcacaccg tacctgacca gtttcgcggg
     3661 cgcgtttttg cgacggtgga gaccatggtc tggtccacga tgatgatctc gatgatgctg
     3721 gccggcattg gatctcaata ctacagtcca cggaccatag gaaccatcgc cggggtgcta
     3781 tcctcctcga cgggcgtgtt ttgggcctgg gcgcatttca cgggccggct gcccgagccg
     3841 gccgagcagg gcgtcgcaga tgaagaagtg gaaatccacg gcgaggcggt cgtttgagcc
     3901 tttgcggccg gcggccgccc gtgtttcgcc ggtgaggcgc ggcctttggg gcgcgcttct
     3961 cctgctgctc gcgtgggccg gcgccgcccg ggcggacgtg cctccgcttc agcccaaggg
     4021 ctatacaaat gactatgcag gtgtcctgag cgcaggcgcg gtggcgcagg cggacgcgct
     4081 ggccgcggaa gtggaacgca agaccggcgc gcagatggcc gtggtaatcg tgaaatccct
     4141 ggatggcgag gccatcgagg attatgcgaa cacgctggcc cgccgctggg gcatcggcaa
     4201 aaaggacaat cgcggtattc tgctgctttt ggcgatacag gagcggcgcg accgcctgga
     4261 ggtcggctac gggctggagc ccatattgcc cgacggcaaa gtcggcggga ttctgcgagg
     4321 gctgcggctt tatttgcagc agggcaatta cgatgccgcc gttctcactg cgcttggcga
     4381 gatttcggcg gtcatcgccc aggaggcggg cgttacactc gaaacgcacg ccctgccgcg
     4441 gcctccggcg gcgcaatatc acagccgcgg ggtagacctg ccttggtggg tgatccttct
     4501 gggcgtcggc gggttggtgc tgtttattct gataacagga atgaatccgc tgttttttct
     4561 gatgggcgga atgccggggt ggggcggccc gagcggccgc gagcgggacg gcaactggga
     4621 cggaggaggc ttcagcggct tcggcggcgg ggattttggg ggcggcgggg cgtcgagcga
     4681 ctggtaatgc cgagccgcac ggccgacccg gctcctggcc agccgccggg cttgcggtga
     4741 gccgcctccg ccgacgcgcc gccaaggaat ccggctttct gtacaagaaa cggggatgac
     4801 gaatctatgg aagatttact aaccgatttg gtcgaacggc tccaacgcgc gtacggcggc
     4861 gagctgctgt cggtggtgct ttacggctcg gccgccacca gcgattatca tgagcagcat
     4921 tcggatctga atgttctctg cgttctggaa cgcgtggggc tgccagagct cgagcgcagc
     4981 ggggaagccc tgcggtggtg gacgaaacag gggcagccca cgcccctgct gctctccgca
     5041 gaggaggtcc gggattccac cgacgttttc cccatcgagt tcctcgacat caaagagagt
     5101 taccgcgttc tgtacggagc ggatctggtg aaggacattc aggtggacat gtccaagcac
     5161 cggacccagc tcgagcacga actgcgttcg cgcctgctgc gcctgcgcaa gcatttcctg
     5221 aagacccagc acgacgacaa ggcggtgagt ttgctgatgc ttgaatcact gccgaccttt
     5281 gccacgctgt tccgccacgt tctgctcgcg gccggatttc cagcgccggt gaagaagcat
     5341 gatatcttcc aggccgccgc gcagcacctg cacgtcggtc cttcgccctt cgacgcgtta
     5401 ctggaagtgc gcaagggcag ccggaagctg gtcgccggag agctgcgttc gtggtttgag
     5461 tcctacctgg cggccattac cgcggtatcg gaaatcgtgg acaaattata agcggaaaga
     5521 agccgctgcc gtcagtgcgc tgtcttttcc gcccggagag tggagacgcg gctcagaaac
     5581 gcgtccagag tgaggcgcgc tgcgctgccc gcccggcggg gtccggcgga gcctgactgc
     5641 gcagccggcg tctcgaccgt tatcaactgg tcgccgcgaa tttcagccaa cgggcggcgg
     5701 cggtggtcgg caatgacatc cactcctgtg agcgggtcgc ggtcaataaa aaatacgccc
     5761 tgccagagcc cgatggtggg cccgaagggc gtgttggtgt cttcggccag aaacagcact
     5821 acgcggtcgc cctcctgaag ctggggaaaa tcggcgagcc attgccgcag gtctccgacg
     5881 gcgcctccga tcatctcgaa ggtgaaggcg gcgggggccg ccccggcaat cgagcgcgat
     5941 tcggcgattt caacgcggct gtgaatgcgc ccggacgctg tatcctggta cgacgtgatc
     6001 cgctcgatgc ggccgacgac gacccggcga gcgagcctgc tgagatcggt gaagccgaga
     6061 tgcacaacag aggaggccga cagaactcct gcggcagcgg ccagcaatat taccttggtc
     6121 ataatgcaat ttcgcattga aaattcaggc ctcccgaact tggcttcgat cttaccatga
     6181 aattcaccga atgttttgga acttcggcgc cgttcagccg atatacagta ggacgccggg
     6241 cattgcatcg ctattttcac atgctggttc tctttttcgc cggcgccgcc gcatgcctgg
     6301 cggcgggaag cgggaccacc gccgatgaca ccgcctgcat gcagggcggc ttcaaactga
     6361 ttacctttgg aaatacgcct gtaatcggga aagcgggcgc gagcttcaac gttttttttg
     6421 attcggctgg cttcgaaggc aattcgttcg actcggcgct gcgctcggcg atgtcgagct
     6481 ggagctcagt aagcgcttcg gcctggcgct ataacttctc cggctatacg gctgcgggag
     6541 catccccgaa cgaccagcag atggaaatca tcaagggcgg gatgtcgttt ccctcgggag
     6601 tgcttgcgac tacgctggtt ggggcgctcg tttccaccgg acagatcacg gattccgata
     6661 cgtttttcaa tcccgcgtat tcgctgagca ccagccccgg ccccaacgac ttcgattttc
     6721 aatcggtggc cctgcatgaa atggggcacg gcctgggcct ggcgcacaac gacggctgca
     6781 acccccagcc aacggtcatg cagtcggcca tcgcgcccgg cgagcagcgc aggaatctgt
     6841 tttcgccgga gctggccgga gtgacttatc tttatcctgg ctccggaggc ggcgggctcg
     6901 gcgtttcgcc ttcctcgctg attttcacgg gcgcggcggg cggagcatcg cccgcgccgc
     6961 aaactcttct ggtctccgca ccgcagggag ccgcctggtc ggcggccgtt gccacaagca
     7021 gcggcggaaa ctggctcagc gtgtctcccc aatctggggc tggtccggcg accctcaccg
     7081 tgaatgtagc agcggcttcg ctggacgcgg gtttttatgc ggggcagatc acggtgagcg
     7141 gcggcggctc ctccagcgcc gtttccgtgt cgctgaatct ggcgggaatc ggcgccagcc
     7201 cgagttcttt gaccttcacc gccactgtag gaggcgcgca gcccccgccg caggggatca
     7261 acctgagcgg agcgccgggc gcggcgtgga cggcggctac atcgactgcc gacggcggca
     7321 actggctcag catttcttcg agcgccggct acctgcccgc gacggtgctg gtttccgtct
     7381 cgacagcggg actcgcggca agcgcctaca ccggctcgat cacgatcagc tccggcggcg
     7441 cgagcggcca agtatccgtc cagcttgccg tgagcagcga agcgccgctt gtggtttcgc
     7501 cggcgcaact ctcgctttcg ggcgccgccg gaagcgcggc gccggtctgc gctccggcgt
     7561 caatccgcgg ctcgggaagc gcatccctgg actggtccgc cagcctcggc gcttcctggc
     7621 tgagcatcca gcccgcctcg ggccgaacgc ccgcctcgat cagcatatgc gcgacgccgg
     7681 gaagtcttcc ggccggcaac tattctgcaa acgtctcgat cacttccgca gccccgaaca
     7741 gcccggtaaa catcgcagtt tcgttctctg ttactgcttc cgtgaccgtc agcagcggcg
     7801 gagtggtgaa tggggcgtcg ttcaccggcg gccagccgat cgccgcgggt gaaattctga
     7861 gcatcttcgg cgctaatctg gcctcgcaaa cggcgagcgc ggcgggcttt ccgctcccga
     7921 cggcgctggc cgactgccgc gtgcagatcg ccggcgttcc ggccccgctc ctctatgtct
     7981 cgccaggaca gatcaatctg gtcgcgccaa cggcgttgag caatctgtcc gggtccagca
     8041 cgacgctggt agttttcaat ggccttctgg cgtcccagcc gatccgggtt tcagtcgcgg
     8101 gccaggcgcc aggcgtgttt tccgtgctcg gcaacgccgc cggcgccggc gccgtgacgc
     8161 accaggatgg ctccctggtt agccgctcgg ccccgctggt tcccggtgaa ccgatctcgg
     8221 tataccttac cggcatggga ccgctctcgc cgccagtgcc ggacggcgcg ccggcgccgg
     8281 tcaatccgct ggcgcgggcg ctcaacaacg tacagttgat cctcgacggc cagccggtac
     8341 agccccttta cgccggagcc gcgccgaact ttgccggctt gcaggaggtc gtggcccttg
     8401 tcccggcttc cctttcccac cgcttcccgc agatgagcgt gatcgtgcag ggcgccgcgt
     8461 ccaacccctt cagcgcgggc ggcccgacgc tttacgacgt ttcgccggcc tccgtgcccc
     8521 ccggcgccga tgctacggtg acgctccgcg gcattaatct gccttcgacc agcgctgtgt
     8581 tggcgggcgg cgcgccgctg ccggccacgc tttcgcaatc cgactggcaa accttgcgag
     8641 tcagaattcc agcgcggctg ctggcctccg gatcgctgtc cctggccgtg atggacaccg
     8701 ctgctcccag cgagccgccc agcaattcga tttcgctaag tgtacagtaa ctggcttttg
     8761 cgcccgcgat tgccatccgg tggaacggcg gcgcgggcgg ctcggctata atcggcttcg
     8821 catggggagc ggcaaaccag cggcggccgg cggcgagctc ccgtatacag tcctgcggct
     8881 ggaatcctgg gacgagtttt tacagttgat caccgactcg ccctattcca actgggcgtt
     8941 tcgcgggctg gcggatgaaa gatggccgct ctcgagcaag ctctcccgct actttgagaa
     9001 ttttcgcgtg gatcgccggg cgtggccgga gcaagaggag cgcatcctgc ggattttcaa
     9061 gcgcaaggcg catcactttc tcgcgcagcc gccggatccg gacgacgaat ttcagtggct
     9121 ggccctcatg cagcaccacg gagcgccgac gcggctggtg gacttcacct ggtctcccta
     9181 tgtagccgca tttttcgctc ttgaagccgc caccggcgac gccgccgtct gggcactcaa
     9241 tcccgtaccg ctcagctacg ttcacgaaca gacgattcag ggaggccgcc gcattgcgcc
     9301 gccggacatg aacccgcgcg tcgagggcaa tctgcggaag tattttctca aggctgatat
     9361 tccgttcatt tgggtgggag agccgcaggt aatgaaccgg cggctgattg cgcaatccgg
     9421 gacatttgcg attccgggag ttctaaccca gcccatggaa gaaatcttga gcagctaccc
     9481 ggacccgcag cacacgctgg tgaaattcgt gcttccggcg ggcaaggtgc gaaagcgggg
     9541 tttgcgggag ctctatcgca tgaacatcac caacgcaaca ctgttcccgg acctcgacgg
     9601 cctggcgcgc tcgctggctt acgagctcga atttcactgg cgctacgatc cccagaccat
     9661 ggagcgatat ttggattatt agttccggcg ggccaggatc gcggattcgt tgcgccgggc
     9721 gccgcgccgg agatccgcgc ggatcttggg atgaacctga gcgaaggctg ccgcgatcca
     9781 aggcgccccg cggcaagcgc gaattcacct gccggagcct tccgcccggt ccatgtgata
     9841 aactttcgcc ggctgacgag gttcaggccg ccgaaagtat tcgacggcgg cggattccag
     9901 ccggaacgag gttcgccatg caacgactcg gctgcatctt gctatgctcc ctgttggctc
     9961 tcccggcatt ggcccaatcc tcatcggttt tcatcgaaga tctcacttgg accgaagttc
    10021 gcgacgcgat cgccgctggc aagaccacgg cgatctacta tgctggcagc atcgagcaga
    10081 acggaccagg catggctctc ggcaagcacc tttttctggc gcactacatt gccgggcaaa
    10141 tcgccgaaca gctcggcgac gcgctggtgt atccgaccat gccgttcgct cctgtgggcg
    10201 atccgctgaa aaagacgggt cacatgcgct ttccgggcag cacgagcgtt tcggaagcga
    10261 cgtttgaggg ggtggcgcgc gatgtcgctc tcagcgccat ctccgcgggg ttcaaaaacg
    10321 tcgtcatgat gtgcgaccac ggcggcggcc agaacggcct gaagaaagtc gcggccgaga
    10381 tgaaccagga gtgggggccg aagggcgttc acatctatta cattcccgac ctttactaca
    10441 aagagaaaga gcagatgcgg gattatctga ccaaacacaa gatgcgcatc gaccagcacg
    10501 ccggcgtgga cgacacttcg gaagtcatgt tcatcgaccg cgagcacaaa tggattcgca
    10561 aagacaagct ggcgcccgac gacggcaaaa tgggagtcaa cggcgacccg acgcaagcca
    10621 cgccgagcct gggcaagatg ttcatcgatt tcaagattca ggacgccgcc gcacagatcc
    10681 gcagcttgat gaaatcgaag ccgtgagcct gcgaaagccc gcaccgttcg ggcggagttt
    10741 tcccggaaca aatccggcgt ctttcgcgta tactcatcca gaggcgtttc tacctatgag
    10801 ccttcgagac tttctcgcga agcagtttat cgacgtcatc cagtgggtgg aatcggaaga
    10861 cggcgtcctc gcttaccgtt ttccaatgcg ggacatggaa atccagaacg gcggcaagct
    10921 gacggtgcgc gagtcgcaga tggccgcatt cgtgaacgaa ggccaggtgg ccgacgtttt
    10981 cggccccggg ctgtacacgc tcaacacgca cactttgcct ttgttgacga acctgatgaa
    11041 ctgggacaag gcgttccagt cgccgttcaa atccgacgta tacttcttct ccactcgctt
    11101 gcagaccaac cagcgctggg gcacggccac gccggtgacg atccgcgaca aggaattcgg
    11161 cgccgtccgg ctgcgcgcct acggcattta ttcctaccgg ctcagcgagc cgcgggtctt
    11221 ttatacgaag gtgagcggga cgcgcgacat ctatcatgtg gccgatctcg aaggccaact
    11281 ccgcaatacg attatcggcg tcatgtcgga cacctttgcc caaagcacaa ttcccttcct
    11341 cgacatggcg gccaaccagc tcgagctcgg gcagaagatt cacgggcggt tgcaacccgt
    11401 gttcgcagac cttggcctgg cgctcgatag tttcgtggtg gaaaatctct cgctgcccga
    11461 tgagttgcag aagcggctgg acgagcgaat cggaatgacc atggtcggcg atatgggccg
    11521 gtacacgcag ttccaggtcg cgcaatccat gccgatcgcg gcagccaacg aaggagcggg
    11581 aggagccgcg ggaattggtg cgggtctcgg agccgggctg gctatggggc aagcgatgtt
    11641 ccatgcagcg cggcagccgg cggcggaagc ttctccggcc agccccgctc cggccgggcc
    11701 cgagcctggt cccgcaacct ccggcggcgg agcagcgccg gggccagccc cgggtgcaga
    11761 gaccaagttt tgcctggatt gtggcaagcc gatccctcgc cgggcgaagt tttgccccga
    11821 gtgcggcgcg gcccagcaat agaccgatgt ccgggcggga atgggttgtt gaagcgcacg
    11881 gctgcgatgc gagggcgctg gcagaccttg ccaaattgca gcagcttttc aggcgcctca
    11941 ccggggagct tgccttgcac gcggcgggcg agcctctctg gcatcagttt ccatacaccg
    12001 gcggcatcac tggtctttgc ctgctcacgg aatctcacct cgcctgccac acgttcccgg
    12061 aatatcgatc gctgtgcttg aacctgttct gctgccgccc gcgccccgac tgggatttcg
    12121 aaagctattt caagaccgag tttgcggcat cgagcgtgcg cgtgcggcgg atcgagcgac
    12181 cttacgaatg agctccccct ccgccaactg ccccaactgc ggcgctccga tccggttccg
    12241 gtggtcgagc gccgtgcaga ccacgtgcga tttttgccat tcgatactgg tgcggcgcga
    12301 cgttgatctg gccaaagtgg gggaagtcgc cgatctgccg ccggacagtt ctcccatcca
    12361 aatcgccacg gaaggcgtct accgcaatcg gcccttcgtc gtagtcggac ggattgttta
    12421 cgaatacgac gccggaagct ggaacgagtg gcacatcgtt ttcagcgaca gttcgagcgg
    12481 ctggctttcc gacgcgcagg acgaatacgc cgtttctttc cagaccgcgc cgccccagcc
    12541 tttgccgcct cccgagaaag tggagcgcgg ccaattcgcc ttatggaacg gcgtccgcta
    12601 tcaggtcacc agcctgacgc gcgcgcggta tctcggcgtc gagggcgaga tgccgtttga
    12661 aaattgggac aagcgcgagg ctctgttcgt tgatttgcgc tcggcggacg ggcgtttcgc
    12721 aaccatcgat tacagcgagc agaagccgct gctctttctg ggtgaagcgg tggaatttga
    12781 cgatcttagg ctgaaaaatc tgcgccagtt tgagggctgg tcctgatgcc cgccgagcca
    12841 ccggccacag ggccttccgg atctccgccg ggatctgttt ccagcgtgaa agcgctgaat
    12901 tgcccgaact gtggggcggc cctcaccctc cgcgccttcc aacacaccct gagcgtcgtc
    12961 tgcgcgcagt gtctctccgt tctcgacgcc aaagatccga acctgcaaat tctccagcag
    13021 ttcgaggacc gccagcgggt agtggcgctc attccgctgg gcagccgggg caagtggcgc
    13081 aacgaccttt atgaagtgat cggtttccag gtgcggacga tcaccgacgc cggcgcgcag
    13141 tatagctggc atgagtactt gttattcaac ccttacaagg gctttcgcta cttgaccgta
    13201 tacgacggcc actggaatga tgtaaagccg gtaagcgccg tcccggaacc cgcatcttca
    13261 cgcggcaaga gcgccgtgag gctcctcggc cgcacctatg ttcattttca gactgcgagc
    13321 gcggagacga cgttcgtcat gggggagttt ccctggcgcg tcaaggtggg cgagcttgtg
    13381 gcatgcaccg actatatcgc gcctccctac attctttctt cggagacggt ggaaacggaa
    13441 acgacgtggt ccctgggcga gtacgtcgac ggggcgcggg tctgggaggc attccagctt
    13501 cccggacgcc cgcccaggcc ggccggagtt ttcgcgaacc agccctcgcc ttacgcgggc
    13561 aaactgaagc agctatggtt tctttgcgga attcttctcc tgctgctggc gaacctggcg
    13621 ctgctttttt cgggatttgc gcggaaggaa caggtcttcg atcaccagta caccttttcg
    13681 ccgggagcgc gggacgcgtc gttcgttacg ccgagtttcg atctggccgg gcgaacctcg
    13741 gacgtcgagc tttcgattcg gacaaatctc cgcagcaact gggcgtactt caattttgcg
    13801 ttgatcaacg aggcgacggg ccaggcctat gactttggcc gtgaggtgag caattacccc
    13861 ggcgagggca gtccgaacga cgatgtcctt atcccgtcgg tgccgcccgg gcggtactat
    13921 ctgcgggtcg agcccgagat gagttccagc tcctcctcta cgcggaacag ccgaatgacc
    13981 tacgagctga tactgcgccg cgatgttccc tcgccggcct tttactggat ctgtgtgctg
    14041 ctgatcgtgg cgcctcccgt gttcctgacg cttcgcgccg ccgggtttga gaaatcgcgg
    14101 tggcaggagg ggggcgacat cacggccgaa tcagattccg aaccgggaga agacagctga
    14161 tgggacggtt cttgtacctc gcttacggtt tggcgcttct cggcctgacg ggcgcggcgg
    14221 agtaccgcgg ctggagcctg ctgtcggtca acgaagtcaa gaatgttccg cggacggtgc
    14281 gtgacaatcc cggcgcctac cgctcttttt accatggcag ctcgcgatac gtcggaggaa
    14341 aataatgagt gagttcaacg ctggctatgt gctcaacgcg ctggtgtact ccgtgctggg
    14401 catcgtcatt tttgtggccg ctttcgccat cgtggaccgg atgacgccgt accacctgtg
    14461 gaacgagatc gtccacgaac acaacaccgc gctggcgatc ctgatcggcg cgatgtcgat
    14521 cggcatgtgc atcatcatcg ccgccgcagt acactgagcc tgtcgccgcg gccgggaatc
    14581 cgatgggaga gctgcctgaa tgctcatgaa gcggacgggg cttttcgctg cccggcgaat
    14641 gctggcgccg cttgccaacg cctgaagcgc tttccgatgc ccgtcgtcct tttcatttcc
    14701 gttctgctca tcgccgcctg cggtctgatt tatgaactga tcgccggcac gctcgccagc
    14761 tatctgctcg gggacagcgt ctttcagttc tccaccgtaa tcggcagcta tctgttcgcc
    14821 atgggcctgg ggagcggcct ttcccggtac ttcacgcggg gcctggtggc ccggttcgtt
    14881 tccatcgagc tgatggtcgg cctggccgga ggattctcgt cgtcgattct gtttttggcc
    14941 ttcgcctata cgcctggatt ccgcctgatc ttgtatctcg tagtcatact catcggcgtt
    15001 ctagtcggtc tcgagattcc cttgctgatg cgcattctca aaggccgctt tctattccgc
    15061 gacctggtcg cgcacgtgct gactttcgat tacctcggcg ctctgggagc ttctctgctg
    15121 ttcccgattc tgctggttcc caagctgggc ctggtgcgct ccgcgctgct gttcggcgtc
    15181 atgaatgcgg ccgtcgctct ctggtccacg tttttgttcc gcgaccagct cggctccccg
    15241 cacgctctgc gggcaatgtg cggcgtggtt ctgctggctc tatgcctggg catgctgggc
    15301 gccgaacgga tcacggcggc ggctgacacc agtctttacg ccgacgaggt gattttttcc
    15361 cgccagacgc ggtatcagcg catcgtcctg acaaagtgga aagacgacat ccggttgttt
    15421 ctcagttcgc atttgcagtt cagctcgcgc gacgaatacc ggtatcacga agcgctggtg
    15481 catccgggcc tcgcggcgat tcccgcgcct cgccgggttt tggtgctcgg cggcggggat
    15541 ggcctggcgc tgcgcgagat tctgaagtac gcgaacgtcg agcgagtcac gctggttgat
    15601 ctggacccgg aaatgaccag acttttctca gagaaccctt tcctggcgca gttgaacggg
    15661 gaatccctgc gttcttcccg cgtgcaggtg atcaatgcag atgccttccc gtggctggaa
    15721 agcacccgcg atatgtttga tttcataacc gtcgatttcc ccgaccccac gagctactcg
    15781 ctgggcaagc tctataccac ggccttttac cggctcatgg ccaggcattt gagcgcgcaa
    15841 ggcatggcgg tggtgcagag cacatcgccg ttgttcgccc gccagtcgta ttggtgcatc
    15901 gtcgagacgc tcaagcaggc cggcttgcga acctatccgt accacgtgta tgtaccctct
    15961 ttcggcgaat ggggtttcgt gctcgcaagc cagcgcccct accagccgcc ttcgcagctc
    16021 cccgaggggc tgcgtttcct ttcggcgcaa aatctcgggt ccctgttcga atttcccaaa
    16081 gacatgctgc ccgtcccggc cgagcccaac cggctcaaca accaggtgct cgtgcgttat
    16141 tacgagcagg attggagcga tattgcccgt tgaccggcca ctgggagagc ggacccctcc
    16201 gctgcggggc gggggcgccg ccggcgggcc tgtttcacgc cgccagcttc tcggggccgc
    16261 gctgatcggc ctcacgccca aaggccggcg agccattgca ggctcgttcg tcaacgaggc
    16321 gcacttgctc ggccacaagc tgcgcgacca cggtgcgttc cgcaagccct cgcagacgat
    16381 ccaaatcccg ctggtgatcg tgggcggcgg cgtggccggg ctctctgcgg cgtggcggct
    16441 gcacaagcgc ggtttccacg atttcgttct gctcgaaatg gaaaagcagg ccggcggtaa
    16501 ttcacgctgg ggcgagaacg agatcacggc gtacccctgg gccgcccact acgttcccgt
    16561 gccgggcaag cgggcggcgt tggttcggga gctgtttgcc gaattgggcg tgctcaagga
    16621 cggcaaatgg gaggagcgcg atctatgctt ttctccccag gagcgcctgt tccagtacgg
    16681 gcgctggcag gagggcatcg agcccactgc cggcctctcc tcctcggaaa gggagcagtt
    16741 ccggcgcttc gaagcgcgca tcgaggagtt ccgggccacc ggcgagttca cgattccgat
    16801 ggaggccggc gccggctcgc cgcgcgcccg aaccctgcat ctggatcgcc tttccatgtc
    16861 gagctggctt cgcgaggagg gcttcgactc gccgcacctg aactggtata tcaactacgc
    16921 gacgcgcgac gactatggcg cgccggcatc cgatgtttca gcctgggcag gcgtgcacta
    16981 tttcgccgcg cgcgagccgg aggaaaaggg cccgctgacc tggcccgagg gcaacggctg
    17041 gatcgtcaag cagcttttgg ccaggctcgg cgccttcgtt cgcaccgatt cgccggtcta
    17101 ccgcatcgag cggctcggca gccgctggcg ggcgctgacg gaaaaagcgg agtacatcgc
    17161 cggcgcggtg attttcgcgg ccccgacgtt ccttgcgtct tacatcgtgg aaggcgctgc
    17221 gccggcgaaa gggtttgtgt attctccctg gcttacggcc aacttgaccc tggagcgcct
    17281 ccctcgggaa cagcaaagcg agccggcgtg ggacaacgta atctacgact cgccgggact
    17341 gggctatgtc gtcgccactc accaaacgct gcgaacccac gttgagcgga cggtttggac
    17401 ctattactac gccatggcgc aggccccgcc cgccgagaac cggcggctgc tgctggcgaa
    17461 ggactggggc tactggaagg aattcatcct cgacgatctc gagcgagcgc acaaggacat
    17521 ccgctcctgc gtatcccgga tcgacatcat gcggttgggc cacgccatgg cgcgtcccgt
    17581 agcgggcttt ctgacctcag aggaacgaag gcgggcggcc gggcgctcgg gaagttttct
    17641 tttcgccaac tccgacctca gcggcttttc gatcttcgaa gaggcgcagt atcgcggcgt
    17701 cacagccgcc gaccgcgcct tacagcatct cggcggccgc cgctaagccc atttcggccg
    17761 cgtgccaggg gcgcgcggcc ttcttcccga atcgtcccgc ctgctacatg cgagaggccg
    17821 aagatatgct atccatctcc gtgccgcgcc gcggccgcga aacaaatggg atgcgagtcc
    17881 gcaaaacgcg ctcgcggcgg cgctcttcct gcaagttgcg cacgcgggcc tgcaacaggt
    17941 ccttcaaagc gatcataccg accagcttgc catgtccgcc gcgctcgacc acgggaaaac
    18001 gcgtcaggcc ggtttccgcc atccggtaaa ccaccagccg caagggctcg tcggggtagg
    18061 ctacaacggg gcttgggttc acgaggtccg ccatccgcgc cccggggccg tcggatgatt
    18121 gtcgaagcgc tttctggatg ttgtgccgtg tgacgacgcc caccaagtga tgatctttat
    18181 ccaccaccgg atagaggcgc tgcccgccgc ggccgccgct ggtgcgaagg gaagtcgcca
    18241 gatcgtccag cttcacgtcg gccggcaaag cggcgatgtt ggttcgcatc acttccctta
    18301 caaataaaat ttccagcgga tccaccgcat actcgcagct caggtgatag ccgcgccggc
    18361 tgactttctc ggtaagaatc gaccgccgca ggaccagcac cgtgaaggca tgagcgatga
    18421 tgcaagcgat c
//
LOCUS       DASREK010000046        28624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1344923, whole genome shotgun sequence.
ACCESSION   DASREK010000046 DASREK010000000
VERSION     DASREK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 28624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28624
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344923"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..599)
                     /locus_tag="VFA54_02720"
     CDS             complement(378..599)
                     /locus_tag="VFA54_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49746.1"
                     /translation="MRSHTHKFTIADEDAVHVLLNKVLDHSSRQQRREKLLRAVKDAS
                     RGYETSTARERKAFFHGLLTGYAVALKLW"
     gene            complement(792..1901)
                     /locus_tag="VFA54_02725"
     CDS             complement(792..1901)
                     /locus_tag="VFA54_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS49747.1"
                     /translation="MRVNRLFAVNFLIAVLCLVLPASAAEPQNSALVPVISKPASRTI
                     ELPAEIWPYMTVSLHAKVPGYVEQVLVDRGSVVKEGDLLVTLTAPEMEAQIAEADSKF
                     QAAEADRLQAEAQLAAAQSTYDHLTEAAKTPGAVAGNDLIQAQKQVEAAQAVVNSRRK
                     ASAAAQSALAALKAMHAYLKITAPFDGVVTDRLVHPGALVGPGPDPALLVLQQISHLR
                     VVVPVPEEDVGGILRGANVTFRVPAYPRRIYSGTIARIGHSLDQKTRTMPVELDVFNR
                     DGSLAPGMYPSVRWPVRSRTPALFVPKTSVVTTTERTFVIRDRDGRAEWVDVRQGPGE
                     GDLVEVIGNLKPGDIVVRHGTDEIRPGASVQSLPR"
     gene            complement(1891..5037)
                     /locus_tag="VFA54_02730"
     CDS             complement(1891..5037)
                     /locus_tag="VFA54_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS49748.1"
                     /translation="MRLVLAALRKPLTVVAALIAIALCAALAIQRMPIDIFPQVGDPV
                     IYVAQPYGGMDPAQMEGYLTYYYEYHFLYITGIDHVESKSIQGASLMKLVFHSGTDMA
                     QAMAETVGYVNRARSFMPPGTVPPFITRFDAGSVAVGQLVFSSSGHTQGEMQDYALNR
                     VRPLFATLPGVSAPPPFGGNQRTIVITLDPAKLQQYQISPEEAIAAVNKSTLVMPSGN
                     MWTGKVERIARTNAALGADLSELLNTPVRPGPGTTVYLRDIGTIENGADIITAYAHVN
                     GRRTVYIPVTKRADASTLSVIEAVRAAIPEFKKVVPEDVDVSLQFDQSPYVTDSLRGL
                     VMEGLLGAFLTGLMVLLFLHDWRSVLIVVMNIPFALLSAVILLWATRQTLNIMTLGGL
                     ALAVGVLVDEATVEIENIHTQMLPGVSRARAVVEACSRTALARLLSMFCVLAVFVPSF
                     FMVGVSRQLFVPLSLAVAFSMIASYLLSSSLVPVFSVWLMREAHRGEEREGIFGRIRS
                     LYGRYLERVLRLRWPVVIVYLAAVAGLLYLLPRQIGTELFPDANAPLFRIRLRAPAGT
                     RIEETERIVLRALDVIRREAGEDHVEITSDFVGVVPSSYPVDLIHLFTSGPQEAIIQV
                     ALKPDAPRGEALRERLRESLRRELPGAQVSFEAGDIVTQVMSFGSPTPIEVAVQGISL
                     EDDYSYARKLQAHLSKLSFLRDLQLAQENHYPTLEIKIDRDRTGQFGLSMADVVRSVV
                     PATSSSRFTDPNFWRDPTSGNAFQIQVELPQNRIQSLEEVGSIPVMPPGRSKPLLEDV
                     ASLTPGTMPGEIDRYNGQHVVSLTANIHDITLGEAASKIRQALNAAGAPPRGVSVRFR
                     GEIPPLEQTISGLRIGLALAVLAIFLLLAANFQSLRLALAIVLTVPAVLCGVLLMLRI
                     TGATLNIQSFLGAIMAIGIAVANSILLITFAERARREGRPLMEATREGAMGRLRAILM
                     TAAAMIFGMAPMAVGFGEGGPQAAPLGRAVIGGLIVSTFATLTILPSIYAILQRRASL
                     TSPSLNPLDPSSRYYDAG"
     gene            complement(5060..6451)
                     /locus_tag="VFA54_02735"
     CDS             complement(5060..6451)
                     /locus_tag="VFA54_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS49749.1"
                     /translation="MRRCALWVSLAAGIPGWMGARSLRAQGPGITMAQAVDAALRNYP
                     SIRVSEEQRNAAAAAIRLAQTAYLPRVDGLAQMNRATTNNVFGVLLPQGVIPSISGPV
                     LGTNNVGSTWDSGAGVLVTWQPFDFGLRRANVARAAAERDQAQASVNRTRYEVSAATA
                     DAFLTVIAAEQTAKAARASVDSWEVLLRSIHALAAAQLRPGADESRAQAELAAARTQL
                     AQAEQAIEVARAALAQFVGLNPDQIHLDPGKCVEQLPAEHPEAPLNVGANPLAVEQNA
                     VIAHEQSQLGVLQRTYYPLFLLQGSASARGTGMEINGARLGGWNGLAPTFQNYALGLT
                     VTFPAMDRFALREQEAAQTARIRAGQAQFQAIAVNLQAQFNAALATLDGARKIAANTP
                     IQVSSARTALEQATARYQAGLAPIDDVAQAQRLLVRAEIDDALARLNVWRALLQVNAA
                     RGDIQPFVAEIGP"
     gene            complement(6505..6834)
                     /locus_tag="VFA54_02740"
     CDS             complement(6505..6834)
                     /locus_tag="VFA54_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZS49750.1"
                     /translation="MSKTKFDTQDDRDLYSGLIRLHILHHAAEESIFGLGMAEELARH
                     GYRISPGTLYPLLHGLEKKGYLRSSERRIGKTSRREYRATAFGKKALKAAKLKVRELF
                     GELIEGK"
     gene            6969..7592
                     /locus_tag="VFA54_02745"
     CDS             6969..7592
                     /locus_tag="VFA54_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49751.1"
                     /translation="MSLRDFGMLTDRPPARQPLLAEFEASDRAGSLELPPDNRLMATA
                     KAIEAAMESEQPALVRQACAEFLAAAADFYHVRRPEIRVLSARPIRVREGGWASELFG
                     DYDPATARIRVWMRTAVQKRVTSFGTLFATLCHEFCHHLDFERFRFRDSFHTADFTAV
                     RRRCIITPGARRKSGCFGRRCRAAVGASIGRAPIALPPQHRPAVRPA"
     gene            7770..9557
                     /locus_tag="VFA54_02750"
     CDS             7770..9557
                     /locus_tag="VFA54_02750"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZS49752.1"
                     /translation="MKKLKPVDVVIVGGGWTGLAMAKEITGRTALNVVVLERGPARKT
                     SDYMDMMDEVDNALRHRMMQNIAEETVTHRFSRNETAVPVRQYGHFLPGAGTGGAGEH
                     WTGIAYRFLEDEFVLATAMREKYGASKLPEGLSVQDWGVTYAELEPYYWKAEQMMGVG
                     GKAGNLRGKLIEGGNIFEAPRQQEFPNPPHKTTYTIELFGKAARELGYHPYPTPAATL
                     SQTYTNPDGITRPGCAYCNYCMLYGCMIEAKAQPTNTLLPIVQKRKTFTLRNHAWVRR
                     VIHRNGKAEGVMYMDEHGEEVLQPAHIVVLASWTMNNTRLLYLSKIGDPYDPETAKGT
                     LGKNLTHQARGDSGGVVLFEKRVLGHGGAGGMGFRLSDFDGFNGIDQFPGVLRGGSIG
                     GGGVGGHPISSFGNIPPGAARRNWGSEWKKAALEWHGRIVAAGGFSGEHLAYRQNYMD
                     LDPTYTDKWGDPLLRHTIDWTEHEHKQAAMAGAIAAKLARQMAALAGAKYVQGGGRGG
                     GSRTQRYQVVSYNSTHVQGGAIMGSSPETSVVNTYLQHWKMPNLFVVGGSAFPQNASG
                     NPTLTALAITYRSADALIERYLQHPGVIA"
     gene            9554..10219
                     /locus_tag="VFA54_02755"
     CDS             9554..10219
                     /locus_tag="VFA54_02755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZS49753.1"
                     /translation="MRRREFISIPAKGLGGLLIYSLTGKPVRLQAQDGTVKVPLRFFT
                     AEEAKIVQAACARIFPSDESGPGATEANVVVYIDRQLAGPYGRDKYRYTKGPWVESVP
                     EHGYQGKENPQQIYRANIPKLGADFAALPPEQQDERLEKIQNSLFFRMLRQHTIEGMF
                     CDPMHGGNKDLIGWQLIGYPGPLMSYRDEIGKNDTEPFRGKPRSLAQIVGHPVKPWED
                     EAG"
     gene            10613..10849
                     /locus_tag="VFA54_02760"
     CDS             10613..10849
                     /locus_tag="VFA54_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49754.1"
                     /translation="MEYADELNELARVYELTGPDGRPAKFWAEKFFTDYHEALRYYEA
                     RKSLAQQSGSTEKVYVHSPTQTDREGIWAVCVLK"
     gene            11343..11726
                     /locus_tag="VFA54_02765"
     CDS             11343..11726
                     /locus_tag="VFA54_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS49755.1"
                     /translation="MKRSIRTLVADDMPAMLETLCAFLEAQENIAVVAKARDGREALE
                     QALLEQLDLAILDIQMPQINGFRLAAELRQRFPEMRILLVSADGDLKLHHAARSSGAD
                     GFVAKRRLPIDMPKQIRRLFPDWKA"
     gene            11907..12866
                     /gene="msrP"
                     /locus_tag="VFA54_02770"
     CDS             11907..12866
                     /gene="msrP"
                     /locus_tag="VFA54_02770"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HZS49756.1"
                     /translation="MIVIRKAPEIRYSEITPKHVYLNRRRFLTGGAAALGVAAAWDAA
                     RMVRPGPVRAAGALNNLIKSPFSTTEKLTPYNDITHYNNFYEFGTQKDEPAKRAQKLR
                     TAPWVVSVDGEVAKPAKYDLDTIMKMAPLEERIYRHRCVEAWSIVVPWIGFSLSALIK
                     QVQPTSKAKYVAFQSFYDSRQMLLPMEAGIPFPYVEGLRMDEAMHPLTLLCVGLYGEL
                     LPKQNGAPVRLVVPWKYGFKSIKSIVRIRFVEKQPPATWNLAAPSEYGFYSNVNPEVD
                     HPRWSQAKERRLGEFGKRKTLKFNGYGDQVASLYAGMDLRKYY"
     gene            12928..13578
                     /locus_tag="VFA54_02775"
     CDS             12928..13578
                     /locus_tag="VFA54_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HZS49757.1"
                     /translation="MSIHRILVSKWSKAAAFSLSLTPVLLLAWRGFHGELGANPIEFI
                     THSTGDWTLRFLLITLAITPLRKLTGLADLIRFRRMLGLFAFFYGILHLFTYLWLDKF
                     FDMQDIVKDIAKRPFITAGFTGFMLMLPLAITSTAGWIRRLGGRRWQNLHRLIYVSAL
                     AGVVHYYWLVKSDVRLPLMYAAILTLLLLFRLPALAARSRTQKAPAVFSSRGISES"
     gene            13764..15014
                     /gene="icd"
                     /locus_tag="VFA54_02780"
     CDS             13764..15014
                     /gene="icd"
                     /locus_tag="VFA54_02780"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HZS49758.1"
                     /translation="MESFNDVPVPPEGGVIGFANGRFQVPDHPIIPFIEGDGTGRDIW
                     KASRAVFDAAVELAYGGRRRVVWYEVFAGEKAFSRFKEWLPQGTLDAIRKFRIAIKGP
                     LTTPVGGGFRSLNVSLRQLLNLYACWRPVRYYPGVPSPVKHPEKLNVVIFRENTEDVY
                     AGIEWQQGTPEAKRVIEFLTKEMGKKVREDSGIGIKPISITGSKQLIRRAIRYAIENG
                     HKSVTLVHKGNIMKYTEGAFRDWGYELARQEFPDLCVAEADLQGAAPAGKIVIKDRIA
                     DSMFQQVLLRPDEYEVLACPNLNGDYLSDACAAQVGGLGMAPGANIGDEYGVFEATHG
                     TAPKYADQDVINPSSVILSGSMMFQFMGWKEVTAIIERAMTETIRQKRVTYDLHRLME
                     GATKLKTSEFAKALIENMTIGVPA"
     gene            15144..16079
                     /gene="mdh"
                     /locus_tag="VFA54_02785"
     CDS             15144..16079
                     /gene="mdh"
                     /locus_tag="VFA54_02785"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZS49759.1"
                     /translation="MRNKVTVVGSGNVGASCALRLADKELADVVMVDIIEGVPQGKAL
                     DILQSGPVEGVDVHVTGSNDYQPTANSDVVVITAGFPRKPGMSRDDLLWANYDIVKAT
                     VEQVVKYSPSAILILVTNPLDAMCQVAYKVSGFSKNRVIGMAGVLDTARFRTFIAAEL
                     DVSVENVVAMVLGGHGDTMVPLVHSTNVAGIPLTELLDKAAIDRIVNRTRNGGAEIVK
                     YLKTGSAYYAPSSSVVEMVESILKDKKKILPCAAYLEGEYGIEGLFVGVPVKLGARGI
                     EKIYEIKLSEEERAALQKSAAAVKELVDVMKPKLG"
     gene            complement(16158..17237)
                     /locus_tag="VFA54_02790"
     CDS             complement(16158..17237)
                     /locus_tag="VFA54_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS49760.1"
                     /translation="MGQEEAIWNVVSVYQTYVAGMNSPGRPVGNFLFLGPTGSGKTRL
                     VEATAESLLGDARAVIKIDCAEFQHSHEIAKLIGSPPGYLGHRETHPLLSQEVLNQYH
                     TEKVKLSFVLFDEIEKASDALWNLLLGVLDKATLTLGDNRRVDFSRAMVFMTSNLGAA
                     EMSALANPSVGFQQAAVPAGGDAKLAPKITNAGITAARRKFTPEFMNRLDKVVVFRPL
                     GAAELRRILDLELALVQRRVFTNAQGIPFLFSLTDSAKDHLLREGTDLKYGARHLKRA
                     IERSLVFPISNLIATNQVKSGDLIRVDYDPAAKSMSFSRQAEGIPGHVMIEAAEVEPA
                     ELFNIDAAAASVEQPKPAGARSTRK"
     gene            complement(17676..18398)
                     /locus_tag="VFA54_02795"
     CDS             complement(17676..18398)
                     /locus_tag="VFA54_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS49761.1"
                     /translation="MSEPLSGQVALVTGGTRGIGRSIAERLLEAKASVVICGRQLGNV
                     SQAVREMAVRPGGRIEGKACDVARYEDVAELFRFVRDKFGRLDVLVNNAGVGVFKPVD
                     QISSEQWREVVETNLSGAFYCTREAAPLMKQHGGGYIINIGSLAGKNPMNGGAAYNAS
                     KFGLVGLSEASMLDLRYENIRVSYIMPGSVATAFAGAAESGDGWKISPRDIAETVVHL
                     LQSPARTLVSRVEMRPTKPPRR"
     gene            18448..19482
                     /locus_tag="VFA54_02800"
     CDS             18448..19482
                     /locus_tag="VFA54_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49762.1"
                     /translation="MSDGREEIIQTVIDETLDRPFAILRRQLAERLSREFAAAEEKAA
                     QAADGARNMAVEALEHAVRRIHQASSITEVGVALLETASPYCGRAALLVHKGECFVGW
                     RTGKAIAGNDLADAWARLQIPLSKAPALAQVVESRDAVISLRLADHLSPELVEILSAA
                     PEKKAYLFPLLLRQKVVAILYADGSAEADGVQAAALSLLCSVAEAWIEVLSGRPSAAP
                     QREPGGPARLELSAAQPRSAPPGWESLPPAERDIHLRAQRFARVLVADLQLYRAPEIR
                     EGKKAGNLYGRLKEEIDKSREVYQRKFGPSLTGGVDYFHLELVHSVAGDQEELLGPDY
                     PGPIAEPIFG"
     gene            19503..21677
                     /locus_tag="VFA54_02805"
     CDS             19503..21677
                     /locus_tag="VFA54_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZS49763.1"
                     /translation="MRRTLALVCLLAFSRWHAGAQTDRLGPLARKLIETDSPASREAL
                     AQFAAQEAAKGSPEAAREAALARFVLGYQDYQDKKFASAAELLTQAASQPSDLSDYAI
                     YYRALALAANGDYAGAAAALADFNQRFPSSPLAASALVQRAENLSFSSQAAEAVDLLL
                     GAPHSNPASLLLLAQVAARANQEVRSVQIYQRIYYEFPTSAEQAQASEALRTLRIRLG
                     VKYPEPPPALRLLRADRLAAAQKYLPARSEYRALSVRLKGLPREQALVRIGACDYYLH
                     AETRAYRWLASLRLTQPEAAAERIHFMAACCRRLKRAEESDRLIAQIGEKYPQSRWHE
                     EALYNAAGYYFVENEPGHYIPYYRTLLNLFPRSRYAAMAQWKIAWHAYLEKSPEAASL
                     LEEHIRRYPSSPVFTDAIYWLGRIEESRSQSSPAGLANARAIYARLIADYPQHYHSLL
                     AQERLSKLPPANDSPSPAIADLLAGLPAAPALPAPEPREWKSYDARIRLLDRLGLSDL
                     ADRELRFRADSPRFAYCAGLELAERAGERGSYHQAIRFLKHYTPGYLAFPLASMPRRY
                     WELLFPMPWREEIENYSKQNNLDPFLVAALIRQESEFNPGAVSRAHALGLMQIMPSTG
                     RKLGRNFGIRALPASRLYKPETSLQLGTLYLRRVLEQYDGKLEVALAGYNAGEHRADR
                     WLGWHSLDDPAEFVESIPFAETRDYVESVLRNTEIYRKIYGD"
     gene            21667..22842
                     /locus_tag="VFA54_02810"
     CDS             21667..22842
                     /locus_tag="VFA54_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZS49764.1"
                     /translation="MATRVFARRTASFTESVIREMTRLAAQHDAVNLAQGYPDFPAPA
                     ELKAAAQQAICDDVNQYAITWGARNFRAAIAAKYRAWYGLDLDPEREITVCCGATEGM
                     IASLLATINPGDEIIIFEPFYENYGPDAELCGASRRIVPLYPPDWRFDREELRRAFGP
                     RTKGLILNTPNNPTGKVFTREELEFIGGLCVEFDALVFTDEIYEHILYRDGGQHYPMI
                     TLPGMRERAVLVNSMSKTYSVTGWRVGWVIAAPDLTGQIRKVHDFLTVGAAAPLQQAG
                     VTALGLPREYYTRLEADYRHRRDLCVQMLERAGFRCFVPGGAYYVMTDISAFGAAADV
                     AFARRLVAEAGVAAVPGSSFYSEASRGAAQVRFCFCKKDSTLQQAGERLQMWARARA"
     gene            22852..24051
                     /gene="glp"
                     /locus_tag="VFA54_02815"
     CDS             22852..24051
                     /gene="glp"
                     /locus_tag="VFA54_02815"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZS49765.1"
                     /translation="MMIFPFAQAREIVERQVCAGRRPPEIETLPLIECAGRILAQPVR
                     ADRNYPPFDRATRDGYALRAQDSPGRLRVIGQVKAGAEFTAGVRPGEAVEIMTGAPAP
                     AGADAVIMVEQTRREGEFVVIERTLRAGENIVPRGSEAPQGCPVLEPGTRIRFPEIAL
                     LASFGCASAAVYRRPRVAILATGDEVVEITEQPRNFQIRNSNAYSLAAQVEQAGAEPM
                     LLPIAPDEPARTRELVEQGLASDLLLLSGGVSMGKYDVVEDVLAGLGAEFYFDGVLIQ
                     PGKPLVFGKARGKFFFGLPGNPLSTMVTFEVFARAAVELLSGASEAPLRFLHARLGSD
                     FRHKPGLTRFLPALLSGRGAGQVVNPVKWQGSGDIVALTRSNALLVVSADKEEWKAGE
                     WMPVLPL"
     gene            24053..24535
                     /gene="moaC"
                     /locus_tag="VFA54_02820"
     CDS             24053..24535
                     /gene="moaC"
                     /locus_tag="VFA54_02820"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZS49766.1"
                     /translation="MKSRLTHYDASGAVQMVDVTEKPATRRTARAHAFVRLQPAVLEQ
                     LPANPKGNPLEIARIAGIAGAKRTADLIPLCHPLPLTHLDVETRLEKDGIEIFTSATT
                     TGPTGVEMEALTAAAVAALTIYDMTKALDKSIEISSLYLLEKTGGKSGAYRRGDRGSE
                     "
     gene            24532..25038
                     /locus_tag="VFA54_02825"
     CDS             24532..25038
                     /locus_tag="VFA54_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HZS49767.1"
                     /translation="MISQGQPQAAVLTISDSAHRGLREDRSGPVVRERLERLGWNVCA
                     VEILPDEKERIASRLATLADGTQIHAIFTTGGTGLAPRDVTVEATRQVMEREIPGFGE
                     LMRARGRETTPLAMLSRATAGVRGRVLIVNLPGSPRGARESLDAIAELLPHTLDLLNG
                     KTEHQEPQ"
     gene            25071..26171
                     /locus_tag="VFA54_02830"
     CDS             25071..26171
                     /locus_tag="VFA54_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS49768.1"
                     /translation="MRVSAFVLLLSLLAPAWCQDPSAEDKGLPDDQYRFGTTLNLVTA
                     PVTVTGPDKRYVAGLVKEQFRVFDNEKEQKILGFDVSFLPISLVICIESSARIEGLLP
                     SIRKTGILYSDLVLGEQGEAAVISFDSRVEVLQEFTKDPDKLKAAISKVKAGSDATRM
                     SDAVWQAIRMLRTRPDNHRKVIVIVSENRENGSEVHLGESLRDAQLSNIMIYGIRLST
                     VKGRVTRPAETKRSPFPPGISPMPMPPGAVSTPNTQAQSNVEVFNAIPLIIEAVRGVK
                     NLIFNDPLQLLTEGTGGKQLAPLTTPALEDSVSNIGEELRSQYLLTYRPNNLDQGGFH
                     NIRVEVALQGLKVRTRPGYWLGPVPKEASAQN"
     gene            26214..26987
                     /locus_tag="VFA54_02835"
     CDS             26214..26987
                     /locus_tag="VFA54_02835"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZS49769.1"
                     /translation="MDTLRIAGREFQSRLIVGTGKYRSFQQMKRCHELSGADCVTVAV
                     RRVNLTDRSKESLLDYIDRNKIFILPNTAGCYTADEAIRTARLAREVGLSNWIKLEVI
                     GDEKTLFPDNQGLLEATRVLVKEGFVVLPYTNDDVVNAKRLVDAGAAAVMPLGAPIGS
                     GLGIQNAANIRILREMITSVPLIIDAGVGTASDATIAMELGADAVLMNTGIAAAEDPE
                     KMALAMKHAVEAGRLAYLAGRMEKRLYASASSPLAGVVR"
     gene            complement(27111..27416)
                     /locus_tag="VFA54_02840"
     CDS             complement(27111..27416)
                     /locus_tag="VFA54_02840"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZS49770.1"
                     /translation="MSLNFRKPGARRALAIVLGAMFAALAAYQVWGANGLLALRRRLH
                     EERRWEAQNEALRQQNAALEKRIHELRTDPKAIEKIAREELMLAGSGEKILVAPQKK"
     gene            27466..27801
                     /gene="cutA"
                     /locus_tag="VFA54_02845"
     CDS             27466..27801
                     /gene="cutA"
                     /locus_tag="VFA54_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HZS49771.1"
                     /translation="MTDKIVVYSTCSGAEEAKQLASHLIEQRLAACVNVCRGVDSYYR
                     WQGKVQQEAEVLLVIKTSRELFDRLRTEWERLHSYEIPELVAVPIVACAPDYLNWMES
                     EIGPEKAAQ"
     gene            complement(27806..28171)
                     /locus_tag="VFA54_02850"
     CDS             complement(27806..28171)
                     /locus_tag="VFA54_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49772.1"
                     /translation="MSPRRSLSLVPILFLSLIAASILAADVAGDWTLKVSAPDGDHAA
                     KLSITQEGEKISGALSGEQGQFKLEGTVKGEEIQFTVHYTGDEPMAIPFNGKLQGDKM
                     AGEYKAGDITGNWSAEKAK"
     gene            28407..>28624
                     /locus_tag="VFA54_02855"
     CDS             28407..>28624
                     /locus_tag="VFA54_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49773.1"
                     /translation="MLLALIYAGRHLWLREIGEFLVVSDAPQNADIAVVLAGDGFGRR
                     ILKAAELVRQGYVPRVLVDGPRGMYGFD"
BASE COUNT         5664 a         8749 c         8692 g         5519 t
ORIGIN      
        1 cgtcccggga ttggagacca agagacatca agattcaccg caactcccga atccgtgatc
       61 gtcagtagcg aagccgcccc cgccatgaga gcggcggccg cattcatttc tgcgagtgga
      121 acgagcacaa agtcctgcgc cgtttcgatt tgccgggccg catcgaggtg gacagggtga
      181 cagcggcgct cgataaaggc gtgttgcaga tcttggcgcc caaggcggcc acccagaaca
      241 acaggccaag aagattgccg ctttccgcct aagcagaggg cttctggccg gacgaccggg
      301 aacttctcgg agcgtcatct gaaggcccgc cagcgagccg ccggaaagcg cccggcggac
      361 tcaggcggga gctccattca ccaaagcttc agggcgacgg catagccggt caacaagccg
      421 tgaaaaaagg ccttccgctc ccgggctgtg gaagtctcat aacctcgcga tgcgtccttc
      481 acggcgcgca ataatttctc acggcgctgc tggcgtgaac tgtggtcgag aaccttgttt
      541 aataagacgt ggaccgcatc ctcgtcggcg atggtgaatt tgtgtgtatg gcttcgcatg
      601 aacgacctcc ccaacgggtt tccagtttca ttgtatcgcc ggcgtttgca ggcagaccgc
      661 ctgatcgatc aatagacatg cgtaccgcgc ttttgcacgc ggttggattc ggaacgctcg
      721 ctcagaataa acccctccgg tctgaggcgg gatgaaggga acgcagagac ggtctgtccc
      781 ctcgaagccc cttatcgtgg cagcgactga acagaagcgc ccggccggat ttcatccgtg
      841 ccgtggcgta caacgatatc gccgggcttc aaattcccga tgacctcgac caggtcgcct
      901 tcgccggggc cttgccggac atccacccat tcggcgcgcc cgtcacgatc acggatcacg
      961 aaggtgcgct cggtcgtggt cacaacgctc gtctttggca caaatagcgc aggggtcctg
     1021 ctccgcaccg gccacctgac gctcgggtac atgccggggg caagcgaacc gtcccggttg
     1081 aaaacgtcga gctccaccgg catggtgcgg gttttctggt ccagcgagtg cccgatgcgc
     1141 gcgattgtac cggagtagat gcgccgcgga taggccggaa cccggaacgt gacattcgcg
     1201 ccgcgaagga tgccgccgac gtcctcttcg ggcaccggaa ccacaacacg cagatgtgaa
     1261 atctgttgaa ggaccaggag cgcagggtcg ggtccaggac ccaccaaggc gccggggtgc
     1321 accagccggt cggtcacgac tccgtcaaaa ggcgcggtaa tcttcaaata agcgtgcatg
     1381 gccttcagcg cggcaagcgc cgattgcgcc gcagcgctgg ccttccggcg tgagttcacc
     1441 acggcctggg cggcctctac ttgcttctgg gcttggatta gatcattgcc ggccacggcc
     1501 ccaggggttt ttgcggcttc tgtcaagtga tcgtaagtgc tttgggccgc agccagttgc
     1561 gcttcggcct gaagcctgtc ggcctcggct gcctgaaact tagaatcggc ctcggcgatc
     1621 tgggcttcca tttcgggagc cgtcagcgtc acgagcaggt ccccttcctt cacaacgctt
     1681 ccccggtcca ccagaacctg ctccacatag ccgggcactt tggcatgcag cgagaccgtc
     1741 atgtagggcc agatttccgc tggcaactcg atcgttcgag acgccggttt ggagatcact
     1801 ggaaccaaag cgctgttttg cggctccgcg gcggacgccg gcagaactag gcacaggact
     1861 gcgatgagaa agtttacggc aaaaaggcga ttaacccgca tcgtagtatc ggctggaagg
     1921 atcgagggga ttgagagacg gagaagttaa cgacgcccgg cgctggagga ttgcgtagat
     1981 cgaggggaga atcgtgagcg ttgcaaacgt cgaaacaatc aggcccccga ttaccgctcg
     2041 gccgaggggc gcggcctgcg ggccgccctc gccaaatccg acagccatgg gcgccatgcc
     2101 gaaaatcatc gccgccgcag tcatcaggat tgcgcgcagg cgtcccatgg ctccctcccg
     2161 cgtcgcctcc atcagcggcc ggccttcccg ccgggcgcgt tcggcgaatg tgatcagcaa
     2221 gatcgagttg gcgacggcga ttccaatagc catgatcgcg cccaagaacg actggatatt
     2281 cagcgtggcg ccggtgattc tcagcatgag cagcacgccg caaagcacgg ccggcaccgt
     2341 cagcacaatc gccagagcca gccgcagcga ctggaagttc gccgccagca gaagaaagat
     2401 ggccaggacc gccagcgcca gcccgattcg cagaccagag atcgtctgct ctagcggggg
     2461 aatctcgccg cgaaaacgca ctgacactcc gcggggcgga gcgcccgccg cgttcagcgc
     2521 ttgcctgatt ttcgaagccg cctcgccaag ggtaatgtca tggatattcg ctgtcaggct
     2581 caccacgtgc tggccgttgt accggtcgat ttcgcccggc atcgttcccg gcgtcaggct
     2641 ggctacatcc tccagaagag gcttcgagcg tcccggcggc atcacgggaa tgctccctac
     2701 ttcttccagg ctttggatcc ggttctgagg cagttccact tgaatctgaa acgcgttgcc
     2761 ggaagttgga tcgcgccaga aattcgggtc ggtgaaccgg gaggacgacg tggcaggaac
     2821 taccgagcgt actacgtccg ccatactcag cccgaactgg cccgtccggt cgcgatcgat
     2881 cttgatctcc agagtcggat agtgattctc ctgggccaat tgcaagtccc gcagaaagct
     2941 cagcttcgac aaatgcgcct ggagtttccg ggcgtaggaa tagtcatctt ccaggctgat
     3001 tccctgcacc gccacctcga tcggcgtggg cgaaccgaag ctcatcacct gagttacaat
     3061 gtcgcccgct tcgaaagaaa cctgcgcccc tggcaattcg cggcggaggc tttccctcaa
     3121 cctctcgcga agcgcctcgc cccggggcgc atccggcttc aaagcgactt ggatgatggc
     3181 ttcctgcggt ccactggtga acagatgaat caggtctacc ggataactgg agggcacgac
     3241 gcccacaaaa tcgctcgtga tctcgacgtg atcctcgccg gcttcccttc gaatcacgtc
     3301 cagcgcgcgc agaacgatgc gttccgtctc ctcgatccgc gtaccggccg gagcgcgcag
     3361 ccggattcga aatagaggag cgttcgcgtc cggaaacagc tccgttccga tctgacgcgg
     3421 cagaagatac agcaagccgg ccacggcggc gagatagacg atcacgaccg gccagcggag
     3481 ccgcaggacc ctctccagat agcgcccata aagcgaacgg atacggccaa aaatcccctc
     3541 ccgctcttcg ccgcgatgcg cttctcgcat caaccacacg gaaaataccg gaaccagact
     3601 gctcgatagc aaatacgatg cgatcatcga gaacgcgacg gcaagcgaca gcggcacgaa
     3661 aagctgccgg ctcacgccga ccatgaaaaa ggaaggcacg aataccgcga ggacgcaaaa
     3721 catcgacagc aaacgcgcca aggcggtccg gctgcacgct tccaccacgg cgcgcgcccg
     3781 tgatacgccc ggcagcatct gcgtgtgtat gttctcgatt tccaccgtcg cttcatccac
     3841 caaaacgccc accgccagcg ccagcccgcc gagcgtcatg atattgaggg tctgcctggt
     3901 ggcccagagc aggatgaccg cgctcaacaa ggcaaatgga atgttcatga cgacgatcag
     3961 gacgcttcgc cagtcgtgaa gaaagagcaa caccatgagg cccgtcagga aagcgcctaa
     4021 cagtccctcc atgaccagac cgcgcagcga atcggtgaca tacggcgatt gatcgaattg
     4081 caggctgaca tccacgtcct ccggaacgac ttttttgaat tccggaatcg ccgcccgcac
     4141 cgcctcgatc accgacaacg tcgaagcatc cgcccgcttg gtcactggga tgtacacggt
     4201 ccttcgcccg ttcacgtgcg cgtaggcggt gatgatgtcg gcgccgttct cgatcgtgcc
     4261 gatatcgcgc agatagaccg tcgttccggg gcccggccgg acgggggtgt tgagaagctc
     4321 agaaagatcg gcgcccagag ccgcattagt gcgcgcgatc cgctccactt tgcccgtcca
     4381 catattgccg gagggcatca ccagggtgga cttgttgacc gcggcgatcg cttcctccgg
     4441 agagatttga tactgctgca atttggcggg atcaagcgtg atcacgatcg tcctctgatt
     4501 gcccccgaac ggcggcgggg ccgagactcc aggcagggtc gcgaatagcg gccgcacccg
     4561 gttcagagca taatcttgca tttctccctg cgtgtgcccg gagctgctga aaacaagctg
     4621 cccgacggcg acgcttccgg cgtcaaagcg cgtgatgaag ggcggcacgg ttccgggcgg
     4681 catgaaggac cgggcgcgat tcacatagcc cacggtctcg gccatcgcct gcgccatatc
     4741 cgtgcccgaa tggaacacca gtttcatcag agaagcgccc tgaatgctct tgctctcgac
     4801 gtgatcgatg ccggtaatgt aaagaaagtg atattcatag taatacgtca ggtagccttc
     4861 catctgcgcc gggtccatgc ccccataggg ctgggcgacg taaatcactg gatcgccgac
     4921 ctgcggaaaa atatcgatcg gcatccgctg aatcgccagc gcggcgcaca gcgcgatcgc
     4981 gattaaagcc gccaccaccg taagcggttt gcgaagagcg gctaaaacca gacgcattct
     5041 gttctactcc cgccggcgat caaggaccta tctccgccac gaaaggctga atgtcgccgc
     5101 gagccgcatt gacttgcaga agagcgcgcc agacgttcag tcgcgctaaa gcatcatcga
     5161 tctcggcccg caccagcaga cgctgcgcct gtgcaacgtc gtctatgggc gccagccccg
     5221 cttgataccg ggccgtggct tgttcgaggg ctgtccgggc cgaggaaacc tggatcgggg
     5281 tattcgccgc tatcttgcga gcgccatcca gagtagccag tgcagcgttg aactgcgctt
     5341 gcaggttgac ggcgatcgcc tggaattgcg cttgccccgc ccggatcctt gccgtttgcg
     5401 ccgcctcttg ttcccggagc gcaaaccggt ccatggcggg aaaggtaaca gtgaggccga
     5461 gcgcgtaatt ttgaaaagtg ggcgccagcc cgttccatcc cccgaggcgc gccccattga
     5521 tctccatgcc cgttccgcgc gcagaagccg atccctggag caggaacaac ggatagtatg
     5581 ttcgctgcaa gacgccaagc tggctctgct cgtgcgcgat cacggcgttc tgttccacag
     5641 ccagcggatt ggccccaaca ttcagcggcg cctcgggatg ctcggctggg agctgttcaa
     5701 cgcatttccc cggatccaag tggatctggt cggggttcag gcccacgaac tgggcaagcg
     5761 ccgcacgcgc gacttcgatc gcctgctcgg cctgcgcaag ctgcgtgcgg gcggccgcca
     5821 gttcggcctg cgccctcgac tcatccgccc cgggacgaag ctgggctgcg gccagcgcat
     5881 gaatacttcg cagcaaaact tcccagctat ccacggacgc ccgagcggct ttcgccgtct
     5941 gctccgccgc gattacagtc agaaacgcgt ccgccgtagc cgccgaaact tcataccgcg
     6001 tccggttgac ggatgcctgc gcctggtcgc gttcggcagc ggcccgggcg acattcgcgc
     6061 gccgcaatcc gaagtcgaag ggctgccagg tcaccaggac tccggctccg ctgtcccacg
     6121 tggacccgac attattggtg cccagcacgg gcccggaaat cgagggaatg accccctgcg
     6181 gcagcagaac gccaaaaacg ttgttcgtcg tggcgcggtt catctgcgcc agcccgtcta
     6241 cacgcggcag gtacgcagtt tgggccagac ggattgccgc cgccgcagcg ttcctctgtt
     6301 cttctgatac tcgaatggaa ggatagttgc gcagcgccgc atctaccgct tgcgccatcg
     6361 tgatgccggg cccctgagcc cgcaggctcc tggcgcccat ccagccggga atgccggcgg
     6421 ccaacgagac ccacaacgcg cagcgcctca tggccgcaga tttccggcgc ctctgcgcgg
     6481 ctgccttgat tgccctctcc gccgtcattt gccctcgatc agctctccga acaactcgcg
     6541 aaccttcaat ttggccgctt tcaatgcctt cttcccgaag gctgtcgctc ggtattcacg
     6601 ccgcgacgtc ttaccgattc ttcgctcgga agatcgcagg taaccctttt tttccaggcc
     6661 atgaaggagc ggataaagcg ttcccggact gatccggtaa ccgtggcgcg cgagttcttc
     6721 cgccattccc aaaccaaaaa tggattcctc ggccgcgtga tgcagaatgt gcagccggat
     6781 cagacccgaa taaagatctc ggtcgtcttg ggtatcgaat ttcgttttcg acattcgata
     6841 ttgaatcatc cagaataaga atcgaaaggc cccttgtcaa cctttttatt tcgcacctcc
     6901 ccggccataa aagcgccgcg agcgctttgt cagcaaaaat acaccccggc cccggcgctc
     6961 tgtttagaat gtcccttcgg gattttggaa tgctgaccga tcggcccccg gcccgccagc
     7021 cgcttttggc cgagtttgaa gcgagcgatc gcgccggcag tctcgagctg ccgcccgata
     7081 atcggctgat ggcgacggca aaggcgattg aagcggcgat ggaatccgag cagccggcgc
     7141 tggtccggca ggcctgcgcc gaatttctgg ctgccgcggc agatttctat catgtgcgga
     7201 ggcccgagat ccgggtcctc tcggcgcggc cgattcgcgt tcgcgaaggc ggctgggcca
     7261 gcgagctttt tggggactac gaccctgcca cggccagaat ccgcgtttgg atgcgcaccg
     7321 ccgtccaaaa gcgtgtgacg tcgttcggaa cgctattcgc cactttgtgc cacgagtttt
     7381 gccatcacct ggatttcgag cgattccggt tccgcgactc cttccacacc gcggatttta
     7441 cggccgtgcg gcggcgctgt atcatcacgc ccggggcacg ccgcaaaagc ggctgttttg
     7501 gacgccgatg ccgggccgcc gttggcgcat cgattgggcg cgcaccaatc gcgctgcctc
     7561 cacaacatcg gccggcggtt aggccggcgt gagtttactc tccatgaatc tttcgaaaat
     7621 cgccgcctgg cgcgttctgg aagccacgcc gcgaaaagcg agggcgcccc gcttttctac
     7681 atcatttctt tacacatacc gagaaaagtg cctctgcaag cgccgcgcag cgctgtgcta
     7741 aactggctca caaattggag actgctctca tgaaaaaatt gaagccggta gatgtggtca
     7801 ttgtcggcgg aggctggacc gggctggcca tggccaagga aatcaccggg cgcaccgcgt
     7861 tgaatgtggt ggtgctcgag cggggcccgg cccggaagac ctccgactac atggacatga
     7921 tggacgaagt ggataacgct ctgcgccacc gcatgatgca gaacatcgcc gaggaaaccg
     7981 taacgcaccg tttttcgcgg aacgaaacgg ccgtgccggt ccggcaatac ggccattttc
     8041 tgcccggcgc cggcaccggc ggagccggcg agcattggac gggcattgcc taccggtttc
     8101 ttgaagatga attcgtgctc gccacggcga tgcgcgaaaa gtacggcgcc tcgaagctgc
     8161 cggaaggcct cagcgtgcag gattggggcg tcacctacgc cgagctggaa ccttactact
     8221 ggaaggccga gcagatgatg ggagtgggcg gcaaagcagg caatttgcgc ggcaagctga
     8281 ttgaaggcgg caacatcttc gaagcgcccc ggcagcagga attcccgaac cctccgcata
     8341 aaaccacgta tacgatcgag ctttttggca aagcagcccg cgaacttggg tatcatccct
     8401 atccaacgcc ggcggccact ctcagccaga cctacacgaa tcccgacggc atcacgcgtc
     8461 ccggctgcgc ctattgcaac tactgcatgc tctacgggtg catgatcgag gccaaggcgc
     8521 agcctaccaa cacgcttctg ccgatcgtcc aaaaaaggaa gacgttcacg ctgcggaacc
     8581 atgcctgggt tcgccgggtg attcatcgaa acggaaaagc ggaaggcgtc atgtacatgg
     8641 acgagcacgg cgaggaggtc ttacagcccg cccacatcgt tgtgctggct tcgtggacga
     8701 tgaacaacac gcgcctgctg tacctgtcga aaatcgggga cccgtacgat cccgaaaccg
     8761 ccaagggcac ccttggtaaa aatctgaccc atcaggcgcg cggcgacagc ggcggcgtgg
     8821 ttctgtttga gaaacgggtg ttaggacacg gcggcgccgg aggcatgggg ttccgccttt
     8881 ccgattttga cggtttcaac ggcatcgatc aatttccggg cgtcctgcgc gggggaagca
     8941 tcgggggcgg cggcgtggga gggcatccga tttcgagctt cggcaacatc ccccccggcg
     9001 ctgccaggcg caactgggga tcggaatgga aaaaggcggc gctcgaatgg cacgggcgaa
     9061 ttgtcgccgc gggagggttc tcgggcgagc acctcgctta ccggcagaat tatatggatc
     9121 tcgacccgac ctacacggat aaatggggag atcccttgct gcggcacacg atcgactgga
     9181 cggagcacga acacaagcag gcggccatgg caggcgcgat cgcagccaaa ctcgccaggc
     9241 aaatggcggc tctggcgggc gccaagtatg tccagggagg cgggcggggc ggcggcagcc
     9301 ggactcagcg atatcaggtg gtgagctaca acagcactca tgtgcagggc ggcgccatca
     9361 tggggtcgtc ccccgaaaca agcgtcgtca atacgtactt acagcactgg aagatgccca
     9421 atctcttcgt ggtgggcgga tcggcctttc cgcagaacgc ctcgggcaat ccaacgttga
     9481 cggctttggc catcacctat cgctcggccg acgcgctgat tgaacgctat ttgcagcatc
     9541 caggagttat cgcatgaggc gacgcgaatt catcagcatc ccagccaaag ggctgggagg
     9601 gctattgatt tacagcctta cgggaaaacc ggtccgcctg caagcgcagg atggtacggt
     9661 caaggtcccg ctgcgtttct tcaccgcgga agaggcgaaa attgtacagg cggcttgcgc
     9721 gcggatcttc cccagcgacg aaagcgggcc tggcgcaacg gaagccaacg tggtcgtcta
     9781 catcgaccgc cagcttgccg gcccctacgg ccgcgacaag tatcgctaca cgaaaggccc
     9841 ctgggtggag tccgttccgg aacacggata tcagggcaag gaaaatccgc aacagatcta
     9901 ccgcgcgaac attcccaagt tgggagcgga ttttgccgcc cttccgcccg agcagcagga
     9961 cgagcggctc gaaaaaatcc agaactcgct tttcttccgc atgctgcggc agcacacgat
    10021 cgaaggcatg ttctgcgacc cgatgcacgg cggcaacaag gatctgatcg gctggcagtt
    10081 gatcggctat ccaggcccgc tcatgagcta ccgcgacgag atcggaaaga atgataccga
    10141 accgttccgc gggaagccaa ggagcctggc ccagatcgtt ggccacccag tgaagccttg
    10201 ggaagacgaa gcaggttgag cctcatcgtg tttgccgcaa attcgcgctg cctgctattc
    10261 tcgtcagcca aggcgcgccg accagatttt caacggccgc ggcgggcttt ttccgcactg
    10321 taacggctcc tggcgctgta tcctcgctcg cactggcgta acaaaaaaag aaactgtcca
    10381 gagcggccat tagtctattg gctcgacagg tgctttgcat aaacggcggc ccgcgcttcc
    10441 aagcagccgg cggcctccgg cacggtatga ggaatcgcga atcatgggct gctggaaggc
    10501 cgagcgtgga ttggaccacc tcgccggggt cgctatcacc tctgcgattc acggcagaat
    10561 gacatttctc cagagaaagg cgatgcgcgt acttgtccag cactgaggag ctatggaata
    10621 cgcggatgag ctcaatgagc tcgcaagagt gtacgagttg accggcccgg acggaaggcc
    10681 ggcgaaattt tgggccgaaa agttttttac cgactaccac gaggcgctcc gctattacga
    10741 ggcaagaaaa tccctggccc agcagagcgg ttccaccgaa aaggtgtatg tccattcgcc
    10801 gacccaaacc gacagggagg gcatctgggc ggtttgcgtt ttgaagtagc agcgacgggt
    10861 catcgagccg cttgtgccaa cttcggagag ccggtcgtga tatgtgccgc gcttcacgtt
    10921 ggccctttca gtgcgaggca gcgggcggaa tcccaatctt gctcgaactg gctgtcagcg
    10981 ccattgttgc ggaggcgaac tgaaccgaga ccgggaaaca cgcctgtcgc aaagctttgc
    11041 attttaggat tccggaaatc ggcaacagac cgacttcgct aagccgcttt ctttaaacgc
    11101 cgccgctcga tggcgcatag ccggggatct gtaggcgggc gatgttgggg ccttccagcc
    11161 agctcttcgc tttgcagcat agtcctgccg cgcaggactg tggcgttgtt tcggggacat
    11221 gggttcaaaa gacacagcct ggagaagcca atccaaagcg aaggacttga ggggaagatg
    11281 cgatggaaca cgacgcctgt acggaatctg tcctgccggt tgacccaccc ggctccagtg
    11341 atatgaaacg ctctattcga accttggttg cagacgatat gcccgctatg ctggaaaccc
    11401 tttgcgcctt tctcgaggcg caggagaaca tagccgtcgt cgccaaggct cgtgatggac
    11461 gcgaagcgct ggaacaagcc ctgctggagc agctagacct ggccattctg gacatacaga
    11521 tgccacaaat taacggtttc cggctcgccg cggaactgcg gcagcgattt ccggagatgc
    11581 gcattctcct ggtgagcgcc gatggagatc tgaaattgca tcatgccgcc cgaagcagcg
    11641 gcgccgatgg atttgtcgcc aagaggcggc tacctatcga catgccgaaa caaatccggc
    11701 ggctttttcc agactggaag gcgtagcgtt gacggggaaa cgctgagatt ttagggatgc
    11761 ggggaagggc caaagcgaac tccggaattc acaactgcga ctagcggctg ggcagcttag
    11821 ctgtgcttcg cagcgcagcc gcacctcgcg tgagtacaat ttctggcgcg atcagtgtga
    11881 taatgccaat aggggggcga agggccatga tagtgatccg aaaggcgccg gagatacgat
    11941 attccgaaat aactccgaaa cacgtatatc tgaaccgccg ccgatttctg accggcggcg
    12001 ccgccgcgct cggtgtggcc gccgcctggg acgcggcccg catggtccgg cctgggccgg
    12061 tgcgtgcggc cggagctctg aataatttga taaaaagccc gttcagtacg acggagaagc
    12121 tcacgcctta taacgacatt acgcattaca acaattttta cgagttcggc acccaaaagg
    12181 acgagccggc caagcgggcg caaaaactgc gtactgcgcc ttgggtggtg tcagtcgatg
    12241 gagaggtcgc caagcctgcg aaatacgatc tcgatacgat tatgaaaatg gccccgctgg
    12301 aggagcgtat ttaccggcac cgctgcgtgg aggcgtggtc aatcgtggtg ccgtggatcg
    12361 gcttttccct tagcgctctc atcaagcagg tgcagcccac gtcgaaggct aagtatgtcg
    12421 cgtttcaatc cttttacgat tcgaggcaaa tgcttctgcc tatggaagcc ggtattccgt
    12481 tcccctatgt ggagggcctg cgcatggacg aggccatgca ccccctcacc ctcctgtgcg
    12541 tgggcctcta cggcgagctg ctgcccaagc aaaatggtgc acccgtgcgg ctggtggttc
    12601 cgtggaagta cgggttcaag agcatcaaat cgattgtgag gatccgtttc gtggagaagc
    12661 agccgccggc cacttggaac ctggcggcgc cgagcgagta cggtttttac tccaacgtga
    12721 acccggaggt ggatcatccc cgctggagtc aagcgaaaga gcggaggctg ggcgaattcg
    12781 gcaagcgcaa aactttaaaa tttaacggct atggcgatca ggtcgccagc ctgtacgctg
    12841 ggatggattt gcgcaagtat tattgaacca gcggaacggc cgcagcgcag cctggcctcg
    12901 cgccagcgac ggggaccaac cggcctgatg agcatacacc gtattctagt cagcaagtgg
    12961 agcaaagccg ccgccttttc gctgtcgctg acccccgttc tcctgctcgc ctggcggggt
    13021 tttcacgggg agctcggcgc gaatcccatc gagttcatca cccacagcac gggcgattgg
    13081 acgctgcgtt ttctgctgat caccctcgcc atcactcctc tccgcaaact caccggcctg
    13141 gcggacttga tccggtttcg ccgcatgctg ggcttgtttg ccttctttta cggcattctc
    13201 cacctgttca cctatctctg gctcgacaag tttttcgata tgcaggatat cgtgaaggac
    13261 attgccaagc ggcccttcat cacagcaggc tttacgggat ttatgctcat gctgcccctg
    13321 gccatcacat ccaccgcggg ttggatccgg cgcctcggag gccggcgctg gcagaatttg
    13381 caccggctca tctatgtctc cgccctcgcc ggcgtagtgc attactactg gttggttaaa
    13441 tccgacgtcc ggctgccctt gatgtatgcg gcaatcctca ccctgttgct gttgttccgt
    13501 ttgcccgctc ttgcggccag aagccggacc cagaaagcgc cggcggtctt ttcgagccgg
    13561 ggcatctcgg aatcctgagc cgaatcagct cgcgcgttgc gtcatccccg gcgctcgttg
    13621 cgatatagac ggtgattttc cgtctcattg tctctgctct tcctgtccgg cccgcttccg
    13681 cacgcttgcg gaagcgtctt cggccctccc cgcccggttt ctggtagcat aacgattctt
    13741 gccagcttag tacgggagaa gctatggaat ccttcaatga cgtgcctgtt ccccccgagg
    13801 gtggagtgat tggctttgcg aacgggcgct tccaggttcc ggatcacccg atcattccgt
    13861 ttatcgaagg cgacggaaca gggcgcgaca tctggaaagc gtcccgtgcg gtttttgatg
    13921 cggcagtgga gctcgcctac ggcggccgcc gccgcgtcgt ctggtatgaa gtgttcgcgg
    13981 gcgagaaggc cttcagccgg ttcaaggaat ggctgccgca gggaactctc gacgccatcc
    14041 gcaaatttcg catcgcgatc aaagggccgc tgacaactcc tgtgggcggc ggtttccgca
    14101 gcctcaacgt ttccctccgg caactgctca atctttacgc ctgctggcgc ccggtgcggt
    14161 attacccggg cgttccctcg ccggtaaagc atcccgagaa gctgaacgtc gtcatctttc
    14221 gcgaaaatac cgaggacgtt tacgccggca tcgagtggca gcagggtacc ccggaagcga
    14281 agagggtgat tgaatttctc accaaagaga tggggaagaa ggtgcgcgag gattcgggta
    14341 tcggaatcaa gccgatttcc atcactggat cgaagcaatt gatccgccgc gcgatccgct
    14401 acgccattga gaacgggcac aagagcgtga ccctggtgca caagggcaac atcatgaagt
    14461 acaccgaggg cgctttccgg gactggggtt acgaactggc ccggcaagag tttccagacc
    14521 tgtgcgtggc cgaggccgac ttgcagggag cggctccggc gggcaagatc gtcatcaagg
    14581 accgtattgc ggactcgatg ttccagcagg tcttgctccg cccggacgaa tacgaagtgc
    14641 tcgcctgccc caacctgaat ggggattacc tttccgatgc ctgcgcggca caagtcggcg
    14701 ggctgggcat ggccccgggc gccaatatcg gggacgaata cggcgtcttc gaagcgactc
    14761 acggaacagc ccccaaatac gctgaccagg atgtgatcaa tccctccagc gtgatcctct
    14821 ccggatcgat gatgtttcag tttatgggat ggaaagaggt cacagccatc atcgaacgcg
    14881 ccatgaccga gaccattcgg cagaagcgag tcacatacga cctgcatcgg ctcatggaag
    14941 gggcgaccaa gctcaaaacc agcgagtttg ccaaggcgct cattgagaac atgaccatcg
    15001 gtgtaccggc ttgaggcgtc ggcgcagcgc gcaaaatgcg acgcctgcac gcggcctatg
    15061 cgccggcctt gcctcgtgcc ggaacgcgga atcctgtatt tccggcgctg gattttcatt
    15121 cttttcaact gggagattgt caaatgcgca ataaagtgac ggtagtgggt tcgggcaacg
    15181 tcggagcgag ctgcgccctg cgtctcgccg ataaggaatt ggccgatgtc gtaatggtcg
    15241 atatcataga aggggtgccc cagggcaaag cgctcgatat cttgcagtcc ggcccggtcg
    15301 agggcgtgga cgtgcatgtg accggctcca acgattatca gccgacagcc aactccgatg
    15361 tggtcgtaat cacagcggga tttccgcgca agccggggat gagccgcgac gatctgcttt
    15421 gggcgaacta cgacatagtg aaggccacgg tggagcaagt ggtgaaatat tcgccatcag
    15481 cgattctgat cctggtcaca aatcctctgg acgccatgtg ccaggtggcc tacaaggtca
    15541 gcggcttttc caaaaaccgc gtaatcggca tggcaggagt tctcgacacg gctcgtttcc
    15601 gcacttttat cgccgcagag ctcgacgtat cggtggaaaa cgtcgtcgcc atggtgttgg
    15661 gcggccacgg cgataccatg gtgcctctgg tgcacagcac caatgtggcc ggcatcccgc
    15721 tgaccgaact gctggacaaa gccgctatcg accgcatcgt aaatcgaact cgaaacggcg
    15781 gcgccgagat cgtgaaatat ttgaaaaccg gcagcgccta ttatgcgccg tcctcctccg
    15841 tcgtcgagat ggtggagtcg atcctgaagg acaagaagaa aatcctgcct tgcgccgcct
    15901 acctggaagg cgagtacggc atcgagggat tgttcgtagg cgtcccggtg aagctcggag
    15961 cacgcggcat tgagaagatc tatgagatca agctaagcga ggaggaaagg gccgccctgc
    16021 aaaagagcgc cgccgcggtg aaggaactgg tcgatgtcat gaaacccaaa ctgggatagg
    16081 ccgcgccgga aaccacccgg aatcaagaaa ccaagccgct gccttccgcg tttcgccctc
    16141 gcgctcccgg gcgcagccta cttccgggtc gatcgcgctc cggctggttt cggctgctcc
    16201 acgctggccg ctgccgcgtc aatgttgaat agctccgccg gttccacttc cgcggcttcg
    16261 atcatcacgt gccccggtat gccttctgct tggcggctga acgacatgct tttggcggca
    16321 gggtcatagt ccacgcggat cagatccccg ctcttcacct gatttgtagc gataaggttg
    16381 gaaatcggaa acaccagcga gcgctcaatc gcgcgcttga gatgccgcgc tccgtatttg
    16441 agatcggtcc cttcgcgcaa cagatgatcc ttggcgctgt cggtgaggct gaacaaaaat
    16501 ggaattcctt gagcgttagt gaagacgcgc cgctggacca gcgccagctc cagatccaga
    16561 atgcggcgca actcggctgc gccgagcggc cggaacacga cgaccttatc caaccggttc
    16621 atgaattccg gagtgaattt gcgccgcgcg gcggttattc cggcgttggt tatcttgggc
    16681 gccagcttcg catccccgcc ggccggaacc gccgcttgtt ggaatccgac cgaagggttc
    16741 gccagcgcgc tcatttcggc cgctcccaga ttgctggtca tgaaaaccat ggcgcgggag
    16801 aaatcgacgc gccgattatc gcccagcgtc agagttgcct tgtcgagaac acccaacaag
    16861 agattccaaa gcgcatcgct cgccttttcg atctcatcga acaaaacgaa agagagcttc
    16921 accttttccg tgtggtactg gtttaagacc tcctggctta gcaagggatg cgtttcacga
    16981 tgcccgagat acccgggagg cgatccgatc agcttggcga tttcatggct gtgctggaat
    17041 tcggcgcagt cgatcttgat caccgcgcgc gcgtcaccca gcagggactc ggcggtcgcc
    17101 tcgaccagcc gcgtcttgcc cgagccggtg gggcccaaaa agaggaagtt gccaaccggc
    17161 cggccgggac tgttcatgcc cgccacatag gtttgataca cgctcaccac attccagatg
    17221 gcttcctctt gccccacgat acgcctgtgg aggttttgtt ccagtcgatc tgcttcccgc
    17281 ccggttcgtg taggatccaa taactgaagc gcgctggcca atctgttttc ctcccggcct
    17341 ttctacgaat ccttcctgcg gctgtttcct cccgctatgt ctctggtcaa aaccaaccgc
    17401 cacatcctcc atcggctttc acgaaatata tcggatggat gcccttccct gtgcagcgga
    17461 aaaatgggag cgggcggcga aacccgcacg agcaatgttg cgcgccggtg cgggctggcg
    17521 gctgtggaac gagccatcgc tctcagtgta agagggccgg aggcgcgctt cgctggaatg
    17581 gccgctcaac agactgaaga aaaaggcgaa attctcggac aacagccgtg actggcgagt
    17641 atcgtgcccg ctgtttcgaa agtggagggg ctctctcagc ggcggggagg tttcgtgggc
    17701 cgcatttcca cccggctgac cagagtccgc gcgggcgatt gcaacaggtg tacgactgtt
    17761 tcggcgatgt cgcgcggcga tattttccag ccgtccccgc tctccgctgc gccggcgaac
    17821 gccgtggcca cgctgccggg cataatgtag ctcacgcgga tgttctcata tcgcaaatcc
    17881 agcatcgaag cttcgctgag cccgaccagg ccgaacttgc tggcgttata agcggcgcct
    17941 ccgttcatcg gattcttgcc cgccaggctg ccgatgttga tgatgtaccc gccgccgtgc
    18001 tgcttcatca ggggcgcggc ctcgcgcgtg cagtagaacg ctccggaaag gtttgtttcg
    18061 acgacctccc gccattgttc cgaagaaatc tgatcgaccg gcttgaatac gcccactccc
    18121 gcgttattca ccagaacgtc cagcctcccg aacttgtccc ggacaaaacg aaacagttcg
    18181 gcgacgtcct cgtaccgggc aacatcgcaa gcctttccct caattcgtcc gccgggcctc
    18241 accgccatct cccggacagc ttgggaaaca ttacccagct gccgcccgca gatcaccacg
    18301 ctggcttttg cttctagcag acgctcggcg atgctgcggc caattcctct ggtgcctccg
    18361 gtcaccagcg ccacttgacc cgataacggt tccgacatga gtcctcctca gggctgagtt
    18421 acaatgttag cggtcgcgag tcccagaatg tctgacggac gcgaggaaat tatacaaacc
    18481 gtaatcgacg aaaccctcga ccgcccgttt gctattctgc gccggcagtt ggccgagcgg
    18541 ctttcgcggg aattcgccgc cgccgaggaa aaggctgcgc aggccgcgga tggcgcccgc
    18601 aacatggcgg tggaagctct cgaacacgcg gtccgccgca ttcaccaagc atctagtatt
    18661 actgaagtgg gggtggcgct gctggaaacg gcctcgccct actgcggccg cgccgccctc
    18721 ttagtacaca aaggcgagtg ttttgtcggc tggaggaccg gcaaggccat agccggcaat
    18781 gacttggccg atgcctgggc gcggctccaa attccactat ccaaggcgcc cgcgctggcg
    18841 caggtggtcg agtcccgcga tgcggtgatt tcgcttcgtc tcgccgatca tctctctccg
    18901 gagctggtgg aaattctcag cgcggcgccg gagaagaagg cttatttgtt tccgcttttg
    18961 ctgcgccaga aggtagtggc gattctttac gcagacggct cggctgaagc cgacggcgtt
    19021 caagcggcgg ccctcagcct gctgtgttct gtcgccgagg cctggattga agttctctcc
    19081 ggccggcctt ctgccgcgcc gcagcgagag cctggcggac cggcgcggct tgaactctcc
    19141 gcagcgcagc cccgctccgc gcctcccggc tgggagtcgc tgcccccggc cgagcgggac
    19201 attcaccttc gagcccagcg attcgcaaga gttctggtgg cggatttgca gctctaccgc
    19261 gccccggaga ttcgcgaggg caagaaagcc ggcaaccttt acggccgatt gaaagaggaa
    19321 atcgataaga gccgggaagt ctaccagcgc aagttcgggc cgtcgctcac gggcggggtt
    19381 gattattttc acctggagct ggtgcactcc gtggcgggag atcaggagga gttgctgggg
    19441 ccggattatc ccggccccat tgccgagccg attttcggct gactcggtga attggtctgc
    19501 ttatgcggcg cacgttggcg cttgtttgct tgcttgcgtt ttcgagatgg cacgccggag
    19561 cccaaaccga caggctggga ccgctggcgc gcaagctcat tgaaaccgat tctccggcga
    19621 gccgcgaagc tctggcgcag tttgccgcac aggaagccgc caagggatcc ccggaggcgg
    19681 cccgcgaggc ggcgttggcg cggttcgttc tgggttatca ggactaccag gacaaaaagt
    19741 tcgcctcggc ggccgaacta ctcacccagg ctgcgtcaca gccgagcgac ctttctgatt
    19801 acgccatcta ctaccgcgct ctggcgctgg ccgccaacgg agattatgcc ggggcggccg
    19861 cggcgctggc cgacttcaac cagcgctttc cctccagccc gctggccgca tctgcgctgg
    19921 ttcagcgagc ggaaaacctg agcttcagtt cccaggcggc ggaggcggtc gacttgcttc
    19981 ttggcgcccc tcactccaat ccggcatcct tgctgctgct cgcccaggtg gctgcccgcg
    20041 ccaatcagga ggtccgctcg gtccagattt accagcgcat ctactatgaa ttcccaacct
    20101 cggccgagca ggcccaggcc agcgaagcgt tgcgcacgct gcgcatccga ctgggcgtaa
    20161 aatatcccga gcccccgccg gctctccggc tcctgcgggc cgacaggctg gccgctgcgc
    20221 aaaaatatct ccccgcgcga tccgagtacc gcgcgctttc cgtgcggctg aaaggattgc
    20281 ctcgcgagca ggccttggtg cgcatcggcg cctgcgatta ctatttgcac gcggaaacca
    20341 gggcgtatcg ctggctggcg agtctgcggc tcacccagcc cgaggctgcg gccgagcgga
    20401 tccactttat ggcggcctgt tgccggcgcc tcaagcgcgc cgaagagtca gacaggctga
    20461 ttgctcaaat cggagaaaaa tatccgcaat cgcgctggca tgaagaagct ttatacaacg
    20521 cggctgggta ttattttgtc gagaacgagc ccggtcatta catcccttac taccgcacct
    20581 tgctcaatct gtttccccgc agccgctacg cagctatggc gcagtggaaa atagcctggc
    20641 atgcgtatct ggaaaagagt ccggaggccg cttcgcttct ggaagagcac attcgccgct
    20701 atccgtcgag ccccgtgttc acggacgcga tttattggct gggcagaatc gaggaatcgc
    20761 ggtcgcaatc ctctccagcg gggctggcga acgcccgcgc aatttatgcg cgtttgattg
    20821 ccgattaccc gcagcattac cattcgctgc tcgctcagga acggctttcg aagctgcccc
    20881 ccgcgaatga ttcgccctcg cctgccatcg cagatttgct ggccggcctt ccggcggctc
    20941 ccgcgctccc cgcgccggaa cctcgagagt ggaagagtta tgacgctcgc atccgcctgt
    21001 tggatcgcct cgggctcagc gatctcgccg accgggagct ccgcttccgg gccgattcgc
    21061 cccgttttgc gtattgcgcg ggcctggagt tggctgagcg cgccggcgag cgcggcagct
    21121 atcatcaggc aatccggttt ctgaagcact acactcctgg gtacctcgcc ttccccctgg
    21181 cttccatgcc ccgccggtat tgggagctgc tgtttcccat gccctggcgc gaagagattg
    21241 agaactattc caagcagaac aatctcgacc cgtttctggt tgcggccttg atccggcagg
    21301 aatcggaatt caaccctggg gctgtgtcgc gcgcccatgc tctgggtctg atgcagatca
    21361 tgccgtccac cggccgcaag ctggggcgca atttcggcat acgcgctttg ccggcgagcc
    21421 gcctttacaa gccggaaacg agcctgcaac tgggaaccct gtacctccgc cgggtcctcg
    21481 agcaatacga cggcaagctg gaagtggctc tcgccggcta caatgcggga gagcaccgcg
    21541 ccgaccgctg gctgggctgg cactctttgg acgatcccgc cgagtttgtc gagagcattc
    21601 ctttcgccga gacgcgcgat tacgtggagt ccgttctgcg caacaccgaa atttaccgga
    21661 agatctatgg cgactagagt gttcgcgcgc cgaacggcca gctttaccga gtcggtcatt
    21721 cgggaaatga cgcgcctggc tgcgcagcac gatgcggtga atctggcgca gggctatccg
    21781 gactttcctg cgccggccga actcaaggcg gcggcgcagc aagccatttg cgacgacgtc
    21841 aatcagtacg ccatcacctg gggcgcccgg aatttccggg cggccatcgc cgcgaagtat
    21901 cgcgcctggt atgggctgga tctcgatccc gagcgggaaa tcacggtgtg ctgcggggcg
    21961 accgagggca tgatcgcctc gctgctggcc accatcaatc ccggcgacga aatcattatc
    22021 ttcgagccgt tttacgaaaa ctacgggccg gatgccgaac tgtgcggggc cagccggcga
    22081 atcgtccccc tctatccacc cgactggcgt ttcgaccgcg aagagctgcg ccgcgcgttt
    22141 gggccgcgga cgaagggcct gattctcaac acgccgaata atccgaccgg taaagttttc
    22201 acgcgggaag agcttgaatt catcggcggc ctgtgcgttg aatttgacgc gctcgtgttt
    22261 accgacgaga tttatgagca catcctttac agggacggcg gacagcacta tccgatgatt
    22321 acgctgccgg ggatgcggga gcgcgccgtc ctcgtcaaca gcatgagcaa gacctattcg
    22381 gttacaggct ggcgcgtggg ctgggtgatc gccgcgccgg atcttaccgg ccagattcgc
    22441 aaggtgcacg attttctgac cgtcggcgcg gcagccccgc tgcaacaggc cggcgtgacc
    22501 gcacttggac tgccgcggga atattacacg cggctggaag ctgattaccg gcatcgccgc
    22561 gatctttgcg tgcagatgct cgagagggcg ggcttccggt gtttcgtccc cgggggcgct
    22621 tattatgtga tgaccgacat ttccgcattc ggggcggccg cggacgtcgc attcgcccgg
    22681 cggctggtgg cggaagcggg cgtagcggcg gtgccgggct cgagttttta ctcagaggct
    22741 tcccggggcg ccgcccaggt gcgcttctgc ttctgcaaaa aggacagtac gctccagcag
    22801 gccggagagc gtctccagat gtgggcgcgg gcgcgggcct agttcgcggc catgatgatt
    22861 tttccgtttg cgcaagcccg tgaaattgta gagcggcagg tgtgcgccgg ccgccgtccg
    22921 ccggagattg aaaccctgcc tctcattgag tgcgcgggcc gcatcctggc ccagccggtc
    22981 cgggctgacc gcaactatcc gccttttgac cgcgccaccc gtgacggcta cgccctgcgg
    23041 gcgcaagaca gtcccggccg cctgcgagtt attggacaag tcaaagccgg cgccgagttc
    23101 accgccggcg tgcgtccggg cgaggcggtg gagatcatga cgggcgctcc ggcgccggcc
    23161 ggcgccgatg ccgtcatcat ggtcgagcaa acccgccgcg aaggcgagtt tgtagtgatt
    23221 gagcgcactt tgcgcgccgg cgaaaatatt gtgccgaggg gcagcgaggc cccacagggc
    23281 tgccccgtgc tcgagccggg gacgcggatt cgtttccccg aaatcgcgct gctcgcgagc
    23341 ttcggctgcg cgagcgccgc cgtctatcgc cggccgcgcg tggccattct cgccacagga
    23401 gatgaagtag tcgagattac cgagcagccc cggaacttcc agatccgcaa ctcgaacgct
    23461 tactcgctgg cggcccaggt ggagcaagcc ggcgcggaac ctatgctgct tccgattgcg
    23521 cccgacgagc cggcccgcac gcgcgagctc gtcgagcagg gcctcgcgtc tgatttgctg
    23581 ctgttgtcgg gcggcgtgtc gatggggaag tatgatgttg tggaagatgt gctggcgggg
    23641 ctgggggcgg agttttattt cgacggcgtg ctgattcaac ccggcaagcc gctcgtgttc
    23701 ggcaaagcac ggggaaagtt tttcttcggc ttgcccggaa atcccctctc cacgatggtt
    23761 accttcgaag tgttcgcccg cgcggctgtt gagttgttga gcggcgcaag cgaggctcca
    23821 ctgcgctttc tgcacgcacg cttgggctcg gactttcgcc acaaaccggg ccttacccgc
    23881 ttcctgccgg ccctgctttc gggccggggc gccgggcagg ttgtgaatcc ggtaaaatgg
    23941 caggggtcgg gcgatattgt cgcattgacc cgctccaacg cacttttggt ggtgtcggcc
    24001 gacaaggagg agtggaaggc cggcgagtgg atgccggtgc tgccgctgta aaatgaagtc
    24061 ccgcctgact cattacgacg ccagcggcgc cgtgcagatg gtggacgtga ccgagaagcc
    24121 agccacgcgt cgcacggccc gggcccatgc ttttgtccgg ctccagccgg ccgttctcga
    24181 acaactgccg gccaatccaa aaggaaaccc gctcgaaatc gcgcggattg caggaattgc
    24241 gggcgcgaag cgaaccgccg acctgattcc cctctgccat cccttgccgc tcacgcacct
    24301 ggatgtcgaa acccggctgg agaaggacgg gatcgaaatc tttacctccg ccacgaccac
    24361 cggcccgacc ggcgtcgaaa tggaagccct caccgccgcc gccgtggctg ccttgaccat
    24421 ctacgatatg accaaggcgc tcgacaaatc gatcgagatt tcgagccttt accttctcga
    24481 gaagaccggg ggcaaaagcg gcgcataccg gcgaggcgat agaggttcgg aatgatttcc
    24541 caaggccagc cgcaagccgc cgtcctgact atcagcgact ccgcgcatcg cgggctgcgc
    24601 gaggaccgca gcgggccggt cgtgcgggag cgtctggagc gcctcggctg gaacgtctgc
    24661 gcggtcgaaa ttctgccgga tgaaaaagag cgtatcgcga gccgcctggc gactttggcg
    24721 gatggaaccc agattcacgc catcttcacc accggaggaa ccggtctggc gccgcgggac
    24781 gtcacagtgg aagcgacccg gcaggtgatg gagcgcgaga ttcctggatt tggggagctg
    24841 atgcgcgcgc gggggcgcga aacaacgccg ctcgccatgc tctcccgagc gacggccggc
    24901 gtccgcggcc gtgttctgat tgtgaacttg ccgggttccc cgcggggcgc ccgggaatct
    24961 ctggacgcga tcgcggaact gttgccccac acactggatt tgctcaacgg gaagaccgag
    25021 caccaagaac cgcaatgaaa cgagcacctc ggcgaacccg tccaagtttt gtgagggtta
    25081 gcgcattcgt actgcttttg tcgctgctcg cgccggcctg gtgccaggat ccctccgccg
    25141 aagacaaagg cctcccagac gatcaatacc ggttcggcac aaccctcaac cttgtcactg
    25201 cgcccgtgac cgtgacgggg ccggacaaac gctatgtagc cgggctggtg aaagagcaat
    25261 ttcgcgtctt cgataacgaa aaggaacaga agatcctcgg attcgacgta agctttctgc
    25321 cgatttcgct cgtcatctgc attgaatcga gcgcgcgcat cgaaggtctt cttcccagca
    25381 tccggaagac aggaatcctg tatagcgacc tggtcttggg tgaacagggg gaggcggcgg
    25441 tcatttcgtt tgatagccgg gtcgaagtcc tgcaagagtt tacaaaggat cccgacaagc
    25501 tcaaggccgc gatcagcaag gtgaaagccg gcagcgacgc cacccgcatg agcgatgcgg
    25561 tatggcaggc gattcgcatg ctgcgcacgc ggcccgataa tcatcgaaag gtgatcgtta
    25621 tcgtttcgga aaaccgtgag aacggaagcg aggttcacct gggagagtct ctgcgggatg
    25681 cgcagctctc gaacatcatg atttacggca ttcgtctttc cacggtgaag ggcagagtca
    25741 ccagacccgc cgaaacgaaa cgttctccgt tcccgccggg tatttctcca atgcctatgc
    25801 cgcccggcgc agtttcgacg cctaacacgc aggctcaatc gaacgtggag gtctttaacg
    25861 ccattccgct catcatcgag gcggtgcgcg gcgtgaagaa tctgatcttc aacgaccctc
    25921 tacaactgct cacggaaggg accggcggca agcagttggc gcctctgacc acgccggccc
    25981 tggaggactc ggtaagcaat atcggagagg agctgcggag ccagtatctg ctcacctacc
    26041 ggcccaacaa cctcgatcag ggcgggtttc acaatattcg cgtcgaagtc gccttgcagg
    26101 gcctaaaggt gcggacgcgg cccggctatt ggctgggtcc cgttcccaaa gaagcctcgg
    26161 cgcagaactg accgttttga gagaacggct ctcgggaagg ctacaatttt tgtatggaca
    26221 ccttgaggat cgccggccgg gaatttcaat cccggctgat cgtagggaca ggaaaatacc
    26281 gcagttttca gcaaatgaag cgctgccacg agctctcggg ggccgactgc gtaacggtcg
    26341 ccgtgcggcg agtgaatctg accgaccgca gcaaagaatc tttgcttgat tatatcgacc
    26401 gaaacaagat tttcattctt cccaacacgg ccggatgtta caccgctgac gaagccatcc
    26461 gaaccgcgcg gctcgcccgc gaagtcggac tttcgaactg gatcaaattg gaagtcatcg
    26521 gcgacgaaaa aacccttttc cccgacaacc agggcttgct cgaagcgacg cgcgtcctgg
    26581 tgaaagaagg ctttgtagtg ttgccctaca ccaacgacga cgtggtgaac gcgaagcgcc
    26641 tggtggatgc gggagcggcc gccgtgatgc ctctgggagc tcccatcggt tccggcctgg
    26701 gcattcagaa tgccgcgaat attcgtatct tgcgcgagat gatcacttcc gtcccgctca
    26761 tcatcgacgc cggcgtcggt acggcgtctg acgcgaccat cgcgatggag ctgggcgcgg
    26821 acgccgtatt gatgaatacc ggcattgctg cggccgagga tccggaaaaa atggctcttg
    26881 cgatgaagca cgcagtggag gcgggccgcc tggcttattt ggcgggccgg atggagaaaa
    26941 gactctacgc ctctgccagc agcccgctcg ctggggttgt gcgctagccg catagctgcc
    27001 gatttgaatc agctcgggct tccgaaggca cgccgatacg gcggtgagtt gataaagcag
    27061 gcaggttgga tacagaaagc agaatatccg gtcgcgggcg tcgcgcttga ttattttttt
    27121 tggggcgcta caaggatttt ttcgccggag cctgctagca tcagctcttc ccgggcgatc
    27181 ttctcgatgg ccttgggatc ggttcgcagc tcgtggattc gcttttccaa ggcagcgttt
    27241 tgctgccgca gcgcctcgtt ctgggcttcc caccggcgct cttcgtggag ccggcggcgg
    27301 agagccaaaa ggccattggc gccccatacc tggtaggccg ccagcgctgc aaacatcgct
    27361 ccgagcacga tggcgagcgc ccgccgcgcg cccggcttac ggaaatttag cgacattgca
    27421 tctattctat agtcataacg acctaaagag ccagagaaat ttaacatgac cgacaaaatc
    27481 gttgtctaca gcacctgctc cggggccgaa gaagccaagc agcttgccag ccacctcatc
    27541 gagcagcgct tggcggcttg cgtcaatgtt tgccgtggag tggattccta ttaccgctgg
    27601 caaggaaagg ttcagcagga agccgaagtg ctcctggtga tcaaaaccag ccgcgagctt
    27661 ttcgaccgcc tgcgtacgga atgggaacgt ctgcacagct atgaaattcc cgaactggtc
    27721 gccgttccca tcgtggcttg cgcgcccgat tacttgaact ggatggaaag cgagatcggg
    27781 cccgagaaag ccgcgcaata acggcctact tcgctttttc ggccgaccag ttgcctgtaa
    27841 tgtcgccggc cttgtattcg cctgccatct tgtcgccctg gagctttcca ttaaaaggaa
    27901 tagccatggg ctcgtcgcca gtgtaatgca ccgtgaactg aatctcttcg cctttcacgg
    27961 taccttcgag cttgaactgc ccctgctcgc cgctcagcgc gccggagatc ttttctccct
    28021 cctgggtgat ggaaagcttt gctgcatgat ctccatccgg ggcgctcact ttcaaggtcc
    28081 agtcgccggc cacatcggcg gcgagtatcg aagccgcgat cagggaaagg aaaaggatgg
    28141 gaacgaggga aagactgcgt cgggggctca ccaaccacct ccagccgatc tctccagtca
    28201 acaattaccg acgttgactg gccttgcgag cacgccgcca ccgcgccgca aggggcagcg
    28261 ggcgcacccg ctcagaaggt gcaaggttaa catatttttt tggcatgcga caattgaaaa
    28321 gtgggccgcg ttgacggaga gatttgagga cgagacagga aggcggcgct agatggagag
    28381 cctggcggaa atgggcgaca gcagctttgc tgttggcgct gatctatgcc ggacgccatc
    28441 tgtggctgcg ggaaatcggc gagtttctgg tggttagcga tgcgccgcag aacgccgaca
    28501 tcgcggtagt tctggccggc gacggattcg ggcgccgcat cctgaaggcg gccgaactgg
    28561 ttcggcaagg gtacgtgcca cgcgtcctgg tcgatggccc ccggggcatg tacgggttcg
    28621 atga
//
LOCUS       DASREK010000047         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1345255, whole genome shotgun sequence.
ACCESSION   DASREK010000047 DASREK010000000
VERSION     DASREK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345255"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFA54_02860"
     CDS             <1..1029
                     /locus_tag="VFA54_02860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZS49774.1"
                     /translation="ERRLDLLDRFSQFALIAAREAVGDSRLHFGDGLAEQTAVVMGAG
                     MAGKTSEDEAYHQLYVEGQKRFSPYLVPRSMANAAASQITMEFGITGPGLTVSTACAA
                     STHAAGLAFGMIRSGQCEVALAGGSEAPFAFGPLKSWEAIRIVAPDTCRPFSRDRKGM
                     VLGEGAAVLVLESEERARARGAKIYAEIAGFGMSSDAGHITQPSEAGAAAAMQAALRD
                     AGIGADAVGYINAHGTGTAANDPTETKAIRRVFGAHADRVPVSSTKSMHGHALGAAGA
                     LELAATVLALHFGILPPTANFTAPGEGCDLDYVPNQARAASVDAALSNSFAFGGLNAV
                     LCVRRWQA"
     gene            1506..2000
                     /locus_tag="VFA54_02865"
     CDS             1506..2000
                     /locus_tag="VFA54_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49775.1"
                     /translation="MNRKGSPRTRHEVRAVAMPITSPPARRATVLLAEEHEKMRELLG
                     RILESSGYRVIPVSNDAEAASAAKRHSEPIPLLLGETRTAALQWTHLRETLKQRHPEM
                     SALLLSDYPDVVVEPDAALLTRPFTPAALLDRVREVLRGDPHPRPASSGSARALTPIL
                     LRAS"
     gene            complement(2054..2689)
                     /locus_tag="VFA54_02870"
     CDS             complement(2054..2689)
                     /locus_tag="VFA54_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS49776.1"
                     /translation="MRILVADDHEVVRRGVRSLLEDQPGWSVAGEAQNGREAVEKAKE
                     LKPDVVLIDISMPELNGLEATRQILKAVPRTEVLILTRHDSEELAHEVLAAGARGYVL
                     KSDAGRNLVAALDAVSQHKPFLTSTVSEMVLDGYLRNREEERAPRSLTPREREIVQLL
                     AEGKSNKEVATTLNISVKTVETHRAKVMQKLNFRSITELIHYAVRNRIIQI"
     gene            complement(2698..>2968)
                     /locus_tag="VFA54_02875"
     CDS             complement(2698..>2968)
                     /locus_tag="VFA54_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS49777.1"
                     /translation="LTNVHRHSGSATAKIRIWQNHQEVLLEVSDRGRGMPLEVVAAHG
                     GARAGHGIGILGMQERVKQLGGRLEIESGDHGTTIRAILPLAMVA"
BASE COUNT          542 a          953 c          903 g          570 t
ORIGIN      
        1 gagcggcggc tcgatctgct cgatcgcttc agccagttcg cgctgattgc ggcacgggag
       61 gcggttggcg attcccggct gcattttggc gacggcttgg ccgagcagac tgcggttgta
      121 atgggcgcgg ggatggccgg caaaaccagc gaggacgaag cctaccacca actctatgtg
      181 gagggccaaa agcgtttcag cccctatctg gtgccgcgtt ccatggcgaa cgccgcggcc
      241 agtcagatca cgatggaatt cggcatcacc ggtcccggcc tcacggtctc gactgcttgc
      301 gccgcttcca cccacgcggc gggtttggct ttcggtatga ttcgcagcgg ccagtgcgag
      361 gtggcgctgg ctggcggaag cgaggcgccc ttcgctttcg gccctttgaa aagctgggag
      421 gcgatccgga tcgtcgcccc tgatacatgc cggcccttca gccgagatcg caagggaatg
      481 gtgttgggcg agggagccgc cgtgctggtg ctggagagtg aggaaagagc cagggcgcga
      541 ggggcgaaaa tctacgcgga gatcgccggc ttcggtatgt cgtccgacgc cggccacatc
      601 actcagccct cggaggctgg cgcggctgcc gccatgcagg ccgctttacg cgacgccgga
      661 atcggcgccg acgccgtcgg ctacattaac gcccacggca cgggaacggc agccaacgat
      721 cccaccgaga ccaaagccat ccgccgcgtc tttggcgcgc atgccgatcg agttcccgtg
      781 agttcgacca aatctatgca cggccatgcc ttgggcgcgg cgggcgccct ggagctggcc
      841 gctactgtat tagcactaca cttcggcatc ttgccgccca cagccaactt caccgctccg
      901 ggcgaaggtt gtgatctgga ttacgttccc aaccaggcgc gggccgccag cgttgacgcc
      961 gctctatcta actcctttgc tttcggcgga ttgaacgccg tgctttgtgt tcgccgctgg
     1021 caagcctagc ggcgaggctc ctacaggaaa tcccgtaggt attcttacgg aatcgggcgt
     1081 ctcccaagtg cagaatccaa cccattggcc tgacccgcgt tttgaagcat cctgatagtg
     1141 acgctgcgtt gcgatgcagc aaaacagacc ggctggcgca aaggaattca catggagacg
     1201 acgtggtggg tatactcggc aaaagcaggc gactcatccc gagacatcga ggtcatcgcc
     1261 tccccgccgg cgtgcgaacc gcaggagcgg aaattgcaga cgcttttgga ggagactctt
     1321 agccgccggc ccgcattgcc ggagtaattt ttcggtttgg ccagctctgc caaaggaaac
     1381 caaaaacaat gaccaactcg caagccgcta agcgtttgct aacactgcct cgggcagcca
     1441 agagggccgg cccgcgaaag gcgcggcacg cgccgccaaa aaggttgcag gggcgcgccc
     1501 gcccgatgaa tcgcaagggc tcccctcgca cccgccacga agtccgggcc gtggcaatgc
     1561 cgatcaccag cccgcccgcc cggcgcgcta ccgtgctgct cgccgaagag cacgagaaaa
     1621 tgcgggagtt gctgggacgt attctcgaga gcagcggtta tcgggtgatc ccggtttcca
     1681 acgatgcgga agcagcgtcg gcggccaaaa ggcattccga gccaattccg ttgctgctcg
     1741 gcgagacgcg caccgccgct ctgcaatgga ctcacctgcg ggaaacgctc aaacagcgcc
     1801 atccggagat gtcggctttg ctgctttctg attatcccga tgtcgttgtc gaacctgacg
     1861 ccgctttgct caccaggccg ttcactccgg ccgccttgtt ggatcgcgtg cgggaggtgc
     1921 tccgcggcga ccctcatccg aggccggcgt cctcaggatc cgccagagcg ctcaccccta
     1981 tattgctccg cgcatcttag ctggaagaga aattcggctg tacgcggcca gggcagtgaa
     2041 ccgcggcgag ttgctagatc tgaatgatcc ggttgcggac ggcgtagtgg atcaactcgg
     2101 taatcgaacg aaagttcagt ttctgcatga ccttggcccg gtgcgtctcg accgtcttca
     2161 cgctgatgtt cagcgtcgtg gccacctctt tgttgctctt gccctccgcc aggagctgaa
     2221 cgatttcccg ttcccgggga gtgagggagc gcggcgctcg ctcctcctcc ctgttccgga
     2281 gataaccgtc gagcaccatt tcggaaacgg tggacgtcag aaaaggcttg tgctggctga
     2341 ctgcgtccaa cgcagccacc aggtttcgcc cggcgtccga cttcaacacg taaccccggg
     2401 cgcccgccgc cagaacttcg tgggccagct cctcggaatc gtgccgggtc aggatcaaga
     2461 cctcggtgcg cgggacggcc ttcagaatct ggcgggttgc ttcaaggccg ttcagctccg
     2521 gcatggaaat atcaatcagc accacatccg gcttgagctc cttggctttt tcgacggctt
     2581 cccgtccgtt ctgcgcttcg ccggcgacac tccatcccgg ctggtcctcc aagagagacc
     2641 gcacgccgcg ccggactact tcatggtcat ccgctacgag aatacgcata atcgtcttta
     2701 agcaaccatg gccaggggca atatggctct gatcgtcgtt ccgtggtctc ccgattcgat
     2761 ctcaagccgc ccaccaagtt gcttcactcg ttcctgcatg cccaaaatgc caatgccatg
     2821 gccggcgcgc gcgcctccat gcgccgcgac aacctcgagc ggcattcccc tgccccggtc
     2881 gctgacctcc agaaggacct cctgatggtt ctgccagatc ctgattttcg cggtggcgct
     2941 gccggagtgc ctgtggacat tggtcagg
//
LOCUS       DASREK010000048         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_193020, whole genome shotgun sequence.
ACCESSION   DASREK010000048 DASREK010000000
VERSION     DASREK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193020"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFA54_02880"
     CDS             <1..180
                     /locus_tag="VFA54_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49778.1"
                     /translation="SKKRRHFLTVNFLDERGQQQAIVLELGKSIVRRTLASLEARTGK
                     RIEYQDDEARRAGTS"
     gene            184..876
                     /locus_tag="VFA54_02885"
     CDS             184..876
                     /locus_tag="VFA54_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49779.1"
                     /translation="MRPLICLCLAGVALAQQPPAPLQPPGAGASDTAPLSAEQIEQEH
                     LRRLLGLRRVYVDRLSGGDSAEQVRDMVISALERSRLFIVTEDESKADAYLRGSAEDS
                     VFTETRASREGITARASGRIGSSRSRYDGDSASGAVGVGDSDDFYSKERKHEAMASVR
                     LVARDGDVLWSSTQESQGAKYKGSAADVADKIGKDLAEAFARARKLATSLAGEGRREP
                     RPGAPPSGKPTP"
     gene            complement(984..2159)
                     /locus_tag="VFA54_02890"
     CDS             complement(984..2159)
                     /locus_tag="VFA54_02890"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase operon protein YhjQ/BcsQ"
                     /protein_id="HZS49780.1"
                     /translation="MLSALVIGPEDNLMREFLDKAAWIRNLRICCHVSRYPPPADLLN
                     LLQQHEPDVGIVSLADYDRASRTISRILSGHTMPIVAVHASCEPQVLLELMQLGVREL
                     WFPPFNTDQMQQTVGRFLAQKTAAPPAGPAGSAAFGSLIAFLPARGGCGATTAAVHTA
                     AALQKRKGSVLLADFDFHNSTIAFWMKATPRHGLQEALERAHWLDSALWKSMVNRIGE
                     LEILTAPQTSAPMVFSSSETCAVLEYARRNYSFVLVDLPDAIYSSCWEVLEQSSQILL
                     VATPEMAGLYLARRKIAQIVDRGIPRDRVRILLNRSSPADLSASQVEKFLSIKVAATF
                     GNHYRAVTAAFSEGRLVPADSRLGIQFDEFAADLAGMPKKKENKTGTWKIRQIFSPA"
     gene            2401..>3282
                     /locus_tag="VFA54_02895"
     CDS             2401..>3282
                     /locus_tag="VFA54_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49781.1"
                     /translation="MFSEHDLLRRFCFASRAVWAGIALMAAGQSARPQPADYYVSLQG
                     DDANPGSADQPWRHIEWAMTRPFLRGGDTIHIRGGVYRPASEPNPSSNSTRDEALIRP
                     AASGEPGRPITVMAEPGESATLSGRFLAADWKPLAPGAPIYYYDYATPLSFPFDHPFQ
                     IAEDGRLLYRAASLDSLNAPGRCFVDPGLHRIYIWTTDSGPPSQHVIDYGASAAGIEF
                     LGGVHDWRLTGFSVAGFKSSGIVIRRGAGSIEIDHMDISFIGAQRPGADPTSGYALAV
                     YDTGGGNFIHENDLHHTL"
BASE COUNT          618 a         1017 c         1040 g          607 t
ORIGIN      
        1 tcgaaaaagc ggaggcattt tctgaccgtc aattttctcg atgagcgggg acagcagcag
       61 gcgatcgtgc tcgaactggg caagagcatc gttcgccgga ccttggccag cctggaggcc
      121 cgaacgggca agcggatcga atatcaggac gacgaagccc ggcgggcggg aacgagctga
      181 cgtatgcgcc cgctgatttg tctgtgtctg gccggcgtgg cgctggccca gcagccgccc
      241 gccccccttc agcctcccgg cgccggcgct tccgatactg ctcccctctc ggccgaacag
      301 atcgagcagg agcatttgcg ccgtctgctc ggcttgcggc gggtgtacgt cgaccggctt
      361 tccggcggcg actcggcgga gcaggtgcgc gatatggtga tctcggctct cgagcgcagc
      421 cggctgttta tcgtcaccga ggacgaatcg aaggccgacg cttacctgcg cggctcggcc
      481 gaagattcgg tttttaccga aacgcgggcc tcgcgggaag gcatcaccgc ccgcgcctcg
      541 ggccgcattg ggagcagccg cagccgctac gacggcgact ccgcctcggg ggccgtcggc
      601 gtgggcgaca gcgatgattt ttattcgaaa gagcgcaaac atgaggccat ggcttccgtg
      661 cggctggtcg ctcgcgacgg cgacgtgctg tggtcctcta cccaggaaag ccagggcgcc
      721 aaatataagg gctcggcggc cgacgtggcc gacaagatcg gcaaggatct cgcggaggcc
      781 ttcgcccgcg cgcgaaagct ggcgacatca ctagcagggg agggccgccg cgagcctcgg
      841 cctggagcgc cgccttcagg aaagccgaca ccgtgagaag ccgaggctgt tgcgtgccgc
      901 cgtgcaacaa gggcggtctt gccagtggca gcggagaccg acgtcctcgc cggcggcttt
      961 cctcgtccgc gggcgtcaaa ccctcaggcc ggagagaaaa tctggcggat tttccaggtt
     1021 ccggttttat tttctttctt tttcggcatg ccggccaaat ccgccgcgaa ctcgtcgaat
     1081 tggatgccca gcctggagtc ggcgggcacc agcctgcctt ctgagaaagc agccgtgaca
     1141 gcgcggtaat gattgccgaa agtggccgcg actttgatgg acagaaattt ctccacttgg
     1201 gaagctgaca agtcggcggg agaagatcga ttcagcagga tacgtacgcg gtcccgggga
     1261 atgccgcgat ccacaatctg agcgatcttg cgccgggcca ggtagagccc cgccatttcc
     1321 ggggtcgcaa ccagcaggat ctggctcgat tgctcgagca cctcccagca cgagctgtaa
     1381 atcgcatccg gcagatcgac tagtacgaaa ctgtaatttc tccgggcgta ttccaaaaca
     1441 gcgcaagttt cggagctgga aaagaccatc ggcgcggaag tctggggggc ggtaagtatt
     1501 tccaattcac cgatgcggtt caccatgctt ttccacaaag ccgaatccag ccagtgagcc
     1561 cgttcgagag cttcctgaag tccgtgcctc ggggtcgcct tcatccagaa agcgatagtg
     1621 ctgttgtgaa aatcaaaatc ggcgagcagc acggagcctt tccgtttttg cagcgccgca
     1681 gcggtgtgga ccgcggctgt agtcgccccg caccccccgc gtgcgggcag aaacgcaatc
     1741 aaagagccaa aagcggccga tcccgccggc ccggccggag gagcagccgt cttctgggcg
     1801 agaaagcggc ccacggtttg ctgcatctgg tcggtattga aaggcgggaa ccacaattcc
     1861 cgcactccca gttgcatcaa ttccagcagc acctgaggct cgcaggaggc atggaccgcg
     1921 acgatgggca tggtatgtcc gctcaaaatg cggctgatcg tgcggctggc acgatcgtag
     1981 tccgccaggc tgacgatgcc cacatcgggc tcgtgttgct ggagcaggtt taaaaggtcg
     2041 gccggcggcg ggtaacggga tacatggcag cagatccgga gattccggat ccaggccgct
     2101 ttgtcgagaa attcccgcat caggttgtct tccgggccga tcaccagcgc actcaacatg
     2161 ttccttgccc tcaaaagagg cgaacaccta gctgcttgtc ttatcggcct aaccggaggc
     2221 tcgctgcaca aaaaatttga ttgcaatccc ggcgtcgaac cgttaaaaaa gaggcgaggg
     2281 ttcgccctgc actttgccgg ggaggccgat ccccggattt ctctttcttt cattcaggtg
     2341 ttgttggccc ggccgatgtt ccgccgctgc ggcgggcgcg acagggccgg gagatctcgc
     2401 atgttttccg agcacgatct gctgcggaga ttttgcttcg cgtcccgcgc cgtgtgggcg
     2461 ggcatcgccc tgatggcggc gggccagagc gcccggccgc aaccggccga ctattacgtc
     2521 agcctgcaag gcgatgacgc gaatcccggc agcgcggacc agccgtggcg tcatatcgag
     2581 tgggccatga ctcggccctt tctgcgcggc ggcgacacga ttcacattcg aggcggcgtc
     2641 taccgtcccg cctccgagcc gaacccctcg agcaacagca cgcgcgatga ggccttgatc
     2701 cgtccggcgg caagcggcga gcccggccgg ccgatcacgg tgatggctga gccgggcgaa
     2761 agcgcgacgc tcagcggccg ttttctggcc gcggattgga aacctttggc tccgggagct
     2821 ccgatttatt actacgacta cgcgacgccg ctatcgtttc ctttcgatca tccgttccag
     2881 atcgccgaag acggccggct gttgtatcgc gccgccagtc tcgacagcct caacgctccg
     2941 ggccgttgct ttgtagatcc cggcctgcac cggatctaca tttggaccac ggacagcggt
     3001 ccgccctcgc agcacgtgat cgattacggc gcgtcagcag ccggcatcga gtttctggga
     3061 ggcgtgcatg attggcggct aactgggttc tcagtggctg gattcaaaag cagcggcatc
     3121 gttatccgca gaggcgccgg atccatcgag atcgaccaca tggatatcag ctttatcggg
     3181 gcgcaacggc ccggcgccga ccccaccagc gggtacgcat tggccgtcta cgacacgggc
     3241 ggcggtaatt ttatccacga aaacgacttg caccacaccc tc
//
LOCUS       DASREK010000049         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2006499, whole genome shotgun sequence.
ACCESSION   DASREK010000049 DASREK010000000
VERSION     DASREK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006499"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFA54_02900"
     CDS             <1..446
                     /locus_tag="VFA54_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HZS49782.1"
                     /translation="NARVLSVQGLPLMKPPYGQITAIDMDKGEILWQIAHGETPDNIR
                     NNPALKGLNIPRTGRPGLLGPLVTKTLVICGEAGFFTTPAGVRGAMLRAYDKATGKEV
                     GAIYMPAPQSGSPMTYMLNGEQYIVLAISGGNYSGELIAFKLPAS"
     gene            604..1041
                     /locus_tag="VFA54_02905"
     CDS             604..1041
                     /locus_tag="VFA54_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZS49783.1"
                     /translation="MKITILGIAAAAAALFTGLGAQEQPSRSVWDGVYTQEQAQRGRG
                     LYGQHCAACHGESLEGVEMAPALTGGDFLDKWTGQTVGDLFERIRTTMPQNKPGRLSR
                     DVNVDITAYILSANDFPAGKTDLPRDTLILKQIRIDATKPEKK"
     gene            complement(1090..3222)
                     /locus_tag="VFA54_02910"
     CDS             complement(1090..3222)
                     /locus_tag="VFA54_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZS49784.1"
                     /translation="MLYHAASPPTLIGALALGAFLLSARIGLKPPAAKTVPKTSTIHG
                     DTRVDEYSWLRERSNPEVIRYLEEENRYTEAVMKHTEPLQAALYEEMRRRIKETDLSV
                     PEKIDNYFYYQKTVEGQQYPVFCRKRDAQDAPEEIILDQNALAAGHNYFRLGVHQPSP
                     NHRLFAFSTDTNGSETYTLALKDLSTGELLKDQIPETYYGLAWGNDNKTFFYTTLDPA
                     KRPYRLFRHTVGTDPAHDKLVYEEKDEKFFVHISKTRSRKYILLTLESETSSEVRYLS
                     ADEPTGEFRVVEPRRPNVEYGVEHHGDRFFIVTNENAKNFKLMDAPVSDPSRRNWKEV
                     IPHREAVKLDGVDAFENHLVIYERENGLRKMRIRNMSTGEIRAVDFPEPVYTFFPATN
                     PEFHTHLLRFNYTSLVTPRSVFDYDMNTGRRELKKQYEVLGGYEPERYQSERVFAQAP
                     DGASVPISLVYKKGLARDGKNPLLLYAYGAYGLSTDPVFSPDRLSLLDRNFIFAIAHV
                     RGGGEMGRSWYENGKLLHKKNTFTDFIACAEHLIAEGYTAKDRLVIYGGSAGGLLIGA
                     VLNMRPELFKIAIAKVPFVDVINTMLDESLPLTVTEFEEWGNPKEKQYYDYMKSYAPY
                     ENVAAKAYPHMLVTTGLNDPRVSFWEPAKWVARLRKVKTDENVLLLKTNMGAGHGGAS
                     GRYDRLKETAFEYAFILDLLGMGSAAAC"
     gene            complement(3189..>4129)
                     /locus_tag="VFA54_02915"
     CDS             complement(3189..>4129)
                     /locus_tag="VFA54_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZS49785.1"
                     /translation="VRPVETLPPWGSSLWVESVSQHLGGNGALTAYTLAKLGTPARLC
                     GLVGEDALGRYALEKLKLVGVDVSEVLLEPGSQTATTVGLINQRAERLFLHLRGASRS
                     LRPEQIQLDACRVRGISYFHLGSFFHMPAIREGGAALLERARAAGLVTSVDTMWDGAG
                     RWMADFGALCPWVDLLFVNQDEARMMAGSSELSQVGEFFRSRGAGIVILKMAGEGCAI
                     AAAGEQFMVPAYDVTAVDTTGAGDCFCGGFLAALRLGFSLREAARFANAVAALSIQQI
                     GGAEGLANLEETLAWMARAPVRKGKTCCITPHHLQL"
BASE COUNT          849 a         1237 c         1200 g          843 t
ORIGIN      
        1 aaaacgcgcg ggtacttagc gtacaaggct tgccgctgat gaagcccccc tatggccaaa
       61 tcacggccat cgatatggac aagggcgaaa tcctgtggca gatcgctcac ggcgagacgc
      121 cggacaatat ccgtaacaac ccggcgctga aaggcttgaa tattccgcgt acgggccgtc
      181 ccggcctttt gggcccgctg gttaccaaga ccctcgtcat ctgtggagag gccggtttct
      241 tcacgacgcc agcgggcgtg cggggagcga tgctccgcgc ctatgacaag gccaccggga
      301 aggaagtcgg cgcgatttat atgccagcgc cgcagagcgg ctctcctatg acgtatatgc
      361 tcaatgggga gcagtacatt gtgctcgcaa tcagcggggg caactattcg ggcgagctga
      421 ttgcattcaa gctgccggcc agctagcatg gcgagctagt ccgagtagcg gcgccgctgc
      481 ctctggtccg gctggcgccc gggatgcggc gagcgtttct tcgttagcct gccgggtttt
      541 ccactgtgga agaatagtaa ctcggctggc tgcaaaggcc agccccgttg acctgacatg
      601 aagatgaaaa tcacgattct gggaattgcg gcggcggccg cggcgttgtt tacggggctg
      661 ggcgcgcagg agcagccctc ccgttcggta tgggatggag tgtacaccca ggagcaggcg
      721 cagcgtggcc gggggctata cggccagcat tgcgccgcct gtcacggtga atctctggag
      781 ggcgttgaaa tggccccggc tcttaccggc ggcgattttc tggacaaatg gacgggccag
      841 accgtgggcg atctgtttga aaggatccga accaccatgc cccaaaacaa gccgggacgg
      901 ctgagccggg atgtcaacgt ggatatcacg gcttacatcc tgagcgccaa tgattttccg
      961 gccggcaaaa cggatttgcc ccgcgacacg ctgattctca agcagatccg gatcgacgcc
     1021 acgaaaccgg agaagaagtg aaacctgcgc cgcgacgaag gagccgagtc ttcggtgtcg
     1081 agtttccgat caacatgcgg ccgcgctgcc cattcccagt aaatccaaaa taaacgcgta
     1141 ctcgaacgcc gtttccttca ggcgatcgta cctgccggaa gcgccgccgt gtccagcgcc
     1201 catgttggtc ttgaggagca gaacgttttc gtctgtcttg accttgcgta gccgggcaac
     1261 ccatttggcc ggttcccaaa aagaaacccg cggatcgttg agtcctgtag tgacgagcat
     1321 atggggatac gccttggcgg ccacattctc gtacggggca taggatttca tgtagtcgta
     1381 atactgtttt tccttgggat tgccccattc ctcgaactcg gtgaccgtca gcggcagcga
     1441 ttcatcgagc atcgtgttga tgacgtcgac aaagggcact ttcgcgattg caatcttgaa
     1501 aagctcgggg cgcatgttaa gcaccgcccc aatcaacaac ccgcccgcgc tgccgccgta
     1561 gatgacgagc cggtccttgg ccgtatatcc ttcggcgatc aaatgctctg cgcaggcgat
     1621 gaaatcagtg aacgtattct tcttgtgcag cagcttcccg ttttcatacc aacttcggcc
     1681 catctcgccg ccgcctcgga catgcgcaat cgcgaaaatg aaattgcgat cgagcaggct
     1741 gagccggtcc ggtgaaaaca ccggatccgt gctcagccca taagcgccat aagcgtacag
     1801 cagcaacggg tttttcccgt cgcgagccag gcccttcttg tagaccagcg aaatcggcac
     1861 cgaggccccg tccggcgcct gggcgaagac ccgctcggac tgatatcgct ccggttcgta
     1921 tcctccgagc acttcgtatt gtttcttcag ttcgcgacgg ccggtgttca tgtcgtagtc
     1981 gaagaccgac cggggcgtca ccagggatgt gtagttgaaa cgcagcaaat gcgtgtgaaa
     2041 ctcagggttt gtcgccggga aaaatgtgta aaccggctcc gggaaatcca ctgcccggat
     2101 ttcgcctgtg gacatattgc gtatgcgcat tttgcgcaaa ccgttttcac gctcgtaaat
     2161 caccagatgg ttctcgaacg cgtccacgcc gtcaagcttg acggcctcgc ggtgtggaat
     2221 cacttccttc caattgcgcc gggaagggtc ggaaaccggc gcatccatca gcttgaaatt
     2281 tttcgcgttt tcattggtga cgataaaaaa tctatcgccg tgatgctcga cgccgtattc
     2341 gacgttgggc cggcgcggct ccaccacccg gaattcgccg gtaggctcgt cggccgacaa
     2401 gtaacggact tccgaagaag tctcgctctc gagcgtcagg aggatatatt tccggcttcg
     2461 ggttttggag atgtgaacaa agaacttctc gtccttttcc tcgtagacca gcttgtcgtg
     2521 ggccggatcg gtccccaccg tgtgccggaa gagccggtac gggcgcttgg cgggatcgag
     2581 cgtcgtatag aagaacgtct tgttgtcatt gccccaggcc agcccgtagt acgtctccgg
     2641 aatctgatct ttcagcagct cgcccgtcga aagatctttc agcgccagcg tgtaagtctc
     2701 ggacccgttt gtgtcggtcg aaaacgcgaa aaggcggtga ttcgggctgg gctgatggac
     2761 ccccagccga aagtagttat gcccggcggc cagggcgttc tggtccagga tgatttcttc
     2821 aggggcgtcc tgagcgtccc ttttccggca gaaaaccgga tattgctggc cttctactgt
     2881 tttttgatag taaaagtagt tatcgatctt ttccggcacg gaaagatccg tctctttaat
     2941 gcgccggcgc atttcctcat agagagcggc ctgcaaaggc tcggtgtgct tcatgaccgc
     3001 ttcggtatag cggttctcct cctccaggta gcggatcacc tcgggattcg atcgctcccg
     3061 gagccaggag tattcatcca cccgggtgtc cccgtggatg gtgctggttt tgggaacagt
     3121 cttcgccgca ggcggcttca agccgattcg agctgacaac aagaatgcgc ccagagccag
     3181 ggccccgatc agagttggag gtgatgcggc gtgatacagc atgtttttcc tttcctcacc
     3241 ggggcccggg ccatccaggc cagtgtttct tcaagattcg ccaggccctc ggcgcctccg
     3301 atctgctgga tcgagagggc cgctacggcg ttggcgaaac gggcggcctc ccggagcgaa
     3361 aatccaagcc gcagggccgc cagaaaaccg ccgcagaagc aatctcccgc cccggttgta
     3421 tccacggccg taacgtcgta ggcgggaacc ataaattgtt cccccgccgc ggcgatcgcg
     3481 cagccttctc cggccatttt taaaataaca atcccggcgc cgcggctgcg gaaaaattcg
     3541 cccacttgag aaagctcgct cgatccagcc atcatccggg cctcgtcctg attcacaaat
     3601 agcaggtcga cccagggaca aagcgcccca aaatccgcca tccacctgcc agcgccgtcc
     3661 cacatcgtgt caacagaggt caccagaccg gcggcacgcg cgcgctcgag cagcgccgca
     3721 ccaccctccc ggatggcggg catatgaaaa aagctgccga ggtggaaata ggaaattccg
     3781 cggactcggc acgcatccaa ctgaatctgc tcagggcgca gactccggct ggctccgcgc
     3841 agatgcagaa acagtcgctc cgcccgctga ttgatgagac ccaccgtggt ggcagtctgg
     3901 gagccaggtt caaggagtac ttcagaaaca tctactccga ccaatttcag cttctccagc
     3961 gcgtatcgcc ccaaggcatc ctctcccacc agcccgcaaa gcctggcagg cgtccccaat
     4021 ttggccaaag tgtaagcggt gagcgcgcca tttcctccga gatgctggga aaccgactcg
     4081 acccagaggc tggatcccca cggcggcagc gtctccaccg gccgcacca
//
LOCUS       DASREK010000050        24118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_521704, whole genome shotgun sequence.
ACCESSION   DASREK010000050 DASREK010000000
VERSION     DASREK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 24118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24118
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521704"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFA54_02920"
     CDS             complement(<1..214)
                     /locus_tag="VFA54_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49786.1"
                     /translation="MAKTLRTLLKAARVLGASGEPHDRRFLRRGDLPPANAGARAPGF
                     PRRCFEVILRNTLEWRNWQTHGTQNPA"
     gene            443..613
                     /locus_tag="VFA54_02925"
     CDS             443..613
                     /locus_tag="VFA54_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZS49787.1"
                     /translation="MAISQELLEILVCPVDKEALELKPDQSGLKCTKCHRVYPIKDDI
                     PIMLIDEATIEE"
     gene            610..1668
                     /locus_tag="VFA54_02930"
     CDS             610..1668
                     /locus_tag="VFA54_02930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZS49788.1"
                     /translation="MIAGNEPVLSRLHAGARVVVIRLRSMGDTVLTTPALRLLKTARP
                     DVQIFVVVERPWDRLLESNDDVAGTVICDRSARWKAAAAIRRVRPELCLNLHGGSTSA
                     ALTAFSGASWRAGFAHFPFRAVYNVAIPRAQEVLRRTANAPVHTAEHLASAAFYLGAP
                     SGEIPAARLFAEAAPRTSPYAVLHVAAAYFTKQWPTARFVELGRWLRETLGLEPILTA
                     GPGQGDLLAGVEGLETSANLSVGGLMSLIKGARLFVGNDSGPAHVAAAFGVPCVVIFG
                     SSNSSVWRPWKTRCEVVETAWTCKPCPGDRCYAFDEPRCILSVELEVVQKAVLAVTGQ
                     KQPGAFAPVTAASTLPHD"
     gene            1661..3466
                     /locus_tag="VFA54_02935"
     CDS             1661..3466
                     /locus_tag="VFA54_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZS49789.1"
                     /translation="MTDFRRLLIYVRPYWLRLAVSVLLMAAVGACHAMIPLLIRPVFD
                     RVLNPSSPEAPVELFHPPGFARGLYLHDLMPASVHNVWTMVAVCILGVFLIKGACDFA
                     GNYLVNYVGFAAVTDLRNQLYESILRQSTAFFRSRHSGRLMSSILNDTDKVQVALSHI
                     LADFLRQSFTIVALLLVVLRTDWKLASVSLTVLPFVLIPTARIGRRIRKTSSSTQDSV
                     AEMNQILQETTAGHSIVRAFGMEAFEIRRFRASAARMFRRNLRYVRQQAVASPIIEMF
                     GAITIVGLLSYARLQIKAETMTAGQFTSFVIALLMMYEPVKRLNGIHNIFQQALGASQ
                     KVFEYLGHEQEVKEAPGAPALPPFRRSIEFENVSFRYPDSPNGLVLDGINLRVNAGEV
                     VALVGPSGAGKTTLVNLLPRFFDPTSGAIRIDGCDIRGVTIRSLREQIGLVTQETFLF
                     DDTVANNISYGRPDLPRELIEQAARAALAADFIADLPLGYDTRIGERGQRLSGGERQR
                     IAIARAILKNAPILILDEATSHLDTESEVLVQRALANLMEGRTVIVIAHRLSTIRRAD
                     KIVVIENGRIAEIGRHEALLANGGPYKRLHELQFQ"
     gene            3547..4446
                     /locus_tag="VFA54_02940"
     CDS             3547..4446
                     /locus_tag="VFA54_02940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZS49790.1"
                     /translation="MPRSMTGFARVQTETSEFCLTLGIKSVNHRSLDLQIRLPPELEP
                     FEMAARQAMKKKITRGYVQVTAALESLVPPRVHINRGLVEAYLSAYRDVAREYGITAE
                     PDLNLLFRLPGIVAFVGADAEKSGGLEKALLEALERALDELTLSREREAAGIVEEMMR
                     RSRSIEAGLAQILRLREGLTSVLAERLAQKISELIKGAAADPQRVLQEAAFMADRTDI
                     SEEVQRLQAHNHQLQEVLAPSSSPGEVGKKLDFLLQEMNREANTILSKTSGIGETGLA
                     ITDLGLAIRAEIEKMREQAMNLE"
     gene            4528..5145
                     /gene="gmk"
                     /locus_tag="VFA54_02945"
     CDS             4528..5145
                     /gene="gmk"
                     /locus_tag="VFA54_02945"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZS49791.1"
                     /translation="MVFIISAPSGSGKSTLVERLLATVENVDFSISYTTRPPRGGEKN
                     GEHYRFVDRQTFEAMRDKGELLEWAEVFGNYYGTPRSALDQAREAGHDLVLDIDVQGA
                     AQLKKQLPEAVTVFILPPSRHELENRLRSRSLDDEAVIARRLRDASKEIRNYAAYDYI
                     LVNDQIDRAAEKLKAIFLAERCRKQRAEGIIQPILQSFEVEKKDD"
     gene            5138..5413
                     /gene="rpoZ"
                     /locus_tag="VFA54_02950"
     CDS             5138..5413
                     /gene="rpoZ"
                     /locus_tag="VFA54_02950"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZS49792.1"
                     /translation="MTENKPVQPEASSNEPAATIPGDPEQSVYRFIIVAAKRARQLQS
                     GARPKIPTQSRKMTRVAIEETRRGLVPFIDPENPPPASEEEYPAADQ"
     gene            5427..6641
                     /gene="coaBC"
                     /locus_tag="VFA54_02955"
     CDS             5427..6641
                     /gene="coaBC"
                     /locus_tag="VFA54_02955"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZS49793.1"
                     /translation="MRIVLGVTGGIAAYKAAELARALQERAHTVEVVMTAHAQEFVRP
                     LTFAALTGQKVITGMFGSSTAEATLESAIEHIALAQRCEAIVVAPASADILAKFAHGI
                     ADDFLTTLYLAYAGPVVMAPAMNSNMWVHPATRANVEELRRRGARIVEPPEGYLACGM
                     IGPGRMSPIDEIVAAVEETLHSQATRRDLEGQTVLITAGPTREPLDPVRYLSNRSSGR
                     MGFALAEEALARGARVIVVAGPTALPPPPGAEVIRVETAAEMRQAVMDRLESATIVIK
                     AAAVADFRPAHRSQQKLKKAAIPPAIELEPTPDILAEIGRRKGTRFLVGFAAETGDLN
                     EEAIRKLKAKNCDMVVANRVGRRGTGFDSEENEALIVTNEGKAIEFPRGSKRSLAARI
                     FDCIYQLRLAHT"
     gene            6798..7412
                     /locus_tag="VFA54_02960"
     CDS             6798..7412
                     /locus_tag="VFA54_02960"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZS49794.1"
                     /translation="MPASLLDHIAAGEPREPLAQIEAELAGCTRCRLHKGRTNLVFGV
                     GNPQAQLVFVGEGPGAEEDAQGIPFVGRAGQLLTQMIENTAAREGLPLKRGDVYICNV
                     VKCRPPENRTPEKDEMDTCSPFLFRQLESIRPKAICVLGGTAARALLNTSTGITQLRG
                     KWQNWRGIPVMPTYHPSFLLRGYNAAYKREAWDDLKKLLHYVYG"
     gene            7579..7676
                     /gene="ffs"
                     /locus_tag="VFA54_02965"
     ncRNA           7579..7676
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFA54_02965"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7831..9588
                     /gene="dnaX"
                     /locus_tag="VFA54_02970"
     CDS             7831..9588
                     /gene="dnaX"
                     /locus_tag="VFA54_02970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZS49795.1"
                     /translation="MYQVIARKYRPQTFRQVVNQQHVKQTLENAVAKGRVAHGYIFAG
                     QRGTGKTTVARILAKCLNCVKGPTITPDLECPSCREIAASSSMDVIEIDAASNRGIDD
                     VRELREAVRYRPSRDRYKVFIIDEAHQITDAAFNALLKTLEEPPEWVVFMLCTTEAHK
                     IPPTIVSRCQNFTFQMVDFDHVVAHLQWICEQEGIEAEADALSVIALAGDGSIRDSLS
                     TLDQAIASFGKTLHAADVRDLLGAVSSEVLDEFAAALRDQSPAGMLELVEKLTARGQN
                     PQHFCRDLTRFIRNLMVARMMGADSRLIAASADERRRLDALASQFSEEDLTRHLQLAL
                     NLYRDLQHAPHPRFHLEMGLLKLVYAGKMVDLEKALAAASSDPQPPAHAPGDAQGPTR
                     GSSTRSRSAAPPPAPGGGRMQAAQPAGTAAVAKRNPPAASPAGAETATGAQPGATASP
                     QDDFRSRLLEALRAEGHEFTADAVEHSAIDLAGGDVHFLAPPDLQFSLESASQLLNEI
                     SGRLLGRRARIRVTLADTTIPPRNVSEGAPTRAGTQESGASGDYGPAGERALAHPQVQ
                     KFLATFGGQIRQVRDLKET"
     gene            9585..9899
                     /locus_tag="VFA54_02975"
     CDS             9585..9899
                     /locus_tag="VFA54_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZS49796.1"
                     /translation="MTVKMPGNMQSIMKQAQKMQEQLQQQIAAIRVEASTGGGMVTVR
                     MDGQKHVLEVKIDPEAVSDVEMLQDMVLAAFNEAAKKVDDEAQSKMGGMLGGLGLPPG
                     MF"
     gene            9977..10576
                     /gene="recR"
                     /locus_tag="VFA54_02980"
     CDS             9977..10576
                     /gene="recR"
                     /locus_tag="VFA54_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZS49797.1"
                     /translation="MNDLAEPMARLIDEFKRLPGIGQKSAQRMAFHVLRSAREDAERL
                     ASALLDVKDRMRLCALCNNISDAETCSICSDSSRNQKVLCVVEEPRNILVIEKTRQFQ
                     GLYHVLHGSLSPLRGIGPEQLKVKSLLERLQRSTIEEVIIATNPTVEGEATAVYLSRL
                     LRPLGVRVTRIGMGIPVGSDLDYVDEVTMLKSIEGRKEI"
     gene            10608..12635
                     /locus_tag="VFA54_02985"
     CDS             10608..12635
                     /locus_tag="VFA54_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZS49798.1"
                     /translation="MSTIVNGAPPQTDLFEPEQELKAPFPGIPTTSDGAGMVVWVETN
                     ITQGACAYPITSSTTMGGGYQVEVANGKRNLWGEELAFIEPESEHSAATTCEGFAVAG
                     GRVTNFTSGQGLVLMKEVLFTIAGKRLPVVFHIGARAITSHSLNVHAGHDDVYAVADC
                     GWGVLYARNAQEAGDMALIARRTAEDTETPFLNVQDGFLTTHTIENVRLPEPEMMKLF
                     VGHPSRRLRNLMDPAIPLMSGVVQNQDSYMKGKIAQRRYYNQVLPTLKKVMREFYEMT
                     GRRYDVVMPYRLSDAEYAIVGSGCMMETAEAAVDYIRDHYGIKVGLLHITCFRPFPSV
                     EIVQALKHCKAISVVERLDIPMMQSNPQLCEMKAAFADAMAETPGYPLLTHMPRFYGG
                     SAGLGSRDVRAGDFIAIVENMRSAKPKMYFTVGIKHETALEVKHDPDVRSPGSFSMRG
                     HSVGGYGSVTTNKVIATIAGEVFGMDVQAYPKYGSEKKGLPTTYYLTIAKKHIRVHSE
                     LEHVEFIPLNDVNAFNLENPLAGLADNGMVFVQSQKTDPAQVWNAVPPWARKFMREKN
                     ARLFALDTVSIAREVSSLADLQQRMQGIVLLGVFLRVTPFQAESGISEEQLFAGVEKA
                     LRKYFGKRGERVVQDNLKAVSRGYHEVIEVPREVMLSTAAIDDANAALRVI"
     gene            12687..14282
                     /locus_tag="VFA54_02990"
     CDS             12687..14282
                     /locus_tag="VFA54_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZS49799.1"
                     /translation="MTETITIPLEARIEDYRANVVDVADFNERVVGAYNNGTAEKGLE
                     ADDQVAKSIIPAGTGALRDFSYIAPDIPVFIGENCVGCMDCVTECPDTAILGKAVEPQ
                     TLEQYLAGITDEAERKKMAEQWAITNKYYTVLEKKGVGGGKFGIFIDPTKCKGCAECV
                     DACGDHNALKMIRKNDENMKWYKAAFDFFKSLPETPSKFINEKALADMMLAERSLLYV
                     GGAGSCMGCGEATALRMMLAATGFVYGRENIGIVAATGCNTVYTSTYPYNPYLVPWTN
                     SLFENAPADAMGVRARWDQLGWKEKRLWVIGGDGAMLDIGFQSLSRMFASGMDIKVLV
                     LDTQVYSNTGGQTSTGSFKGQDAKMSFHGKVIQGKKENRKEIANIAMMHKDVYVAQTT
                     CAHPNHFYKAVMEANAYPGPAIVNVYTTCQPEHGVADNMAEHQAKLAADSRAFPVFIY
                     DPRKGPKFKDRLSLVGNPNVKDDWYVNPKTGEQIDFIYFARTEGRFAKHFDKDGNPSP
                     TLLAAQQDRLENWHLLQDLAGIK"
     gene            complement(14350..15384)
                     /locus_tag="VFA54_02995"
     CDS             complement(14350..15384)
                     /locus_tag="VFA54_02995"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HZS49800.1"
                     /translation="MPIESQPRFPLATLPTPLHELKRLRDALGGPRRCPRILIKRDDL
                     TGLALGGNKARKLEFLVGDALRAGATVLVTTGAEQSNHARMTAAAARVAGLGAALVLT
                     RKPGCGPPQGNLLLDYILGAEVHFVPAGPDPAKATGDGEAAKVAEVTAALEGRGERPY
                     LIPVGGSSPVGACGYVAGTLEIVQQLLAMGEAPSRLYYAAGSRGTQAALVLGAKMYSA
                     PYLLYGVAVSGGEPEKTGRAVRIANETAALLGVGGRVGPEDFVTDQGYFGAGYGIPTA
                     GCLEALRMLARHEGVFLDPIYTGKVMAALIDHIRRGQIDPSETVLFLHTGGAPALFAY
                     AEQLELSVGL"
     gene            15504..15815
                     /locus_tag="VFA54_03000"
     CDS             15504..15815
                     /locus_tag="VFA54_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49801.1"
                     /translation="MIVYTGPIDDGRQLIQFLRREELNLRSLKKLIVSREKTVVLDVN
                     GDGIEFPGLTYGNQILEELLRELGVVFTPQTLHNPSATPGGVKEFRLSARWTWGHDRV
                     M"
     gene            complement(15839..16765)
                     /locus_tag="VFA54_03005"
     CDS             complement(15839..16765)
                     /locus_tag="VFA54_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS49802.1"
                     /translation="MIQRLLPFLSLIALFVGLSISSPPFLTATNLSSVVRQTAVINIM
                     ALGMTLIIVAGGIDLSVGAILAFAGLIGTKIMEQHGAIAAGILAGIAAGFFWGLVNGL
                     LTERLKINPFIVTLGTLGIIRGLALLYSGGLPVVGLPRGFSFLGEGAVLGLPFVLYLL
                     VFCAMLTHFLLQYTKLGRYAYAIGSNREAAVYAGIPVSFHTVAVYAISGALTGLAGMI
                     EASRLMTGQPTAGQGYELQVIAAVVIGGGSLSGGEGSVIGTLIGAFIMGLLANGCDLL
                     GISPYIQQAVIGAVIILAVTLDEVRKRRFVKS"
     gene            complement(16762..18210)
                     /locus_tag="VFA54_03010"
     CDS             complement(16762..18210)
                     /locus_tag="VFA54_03010"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZS49803.1"
                     /translation="MLLELEHILKRFGGVRALEDGNLSVRPGEVHLLLGENGAGKSTL
                     MKIAAGMLQPDGGVMRWRGEPVRLRTPAEAAAIGVAMVHQESLIAPHLTVAENIFLGR
                     EPLRRGMLDRRRMEREADALITEHRFPLQSRWRVERLSPAQKQLVEICRAIHSASSLL
                     IFDEPTSSLSESETAEVFRIVRNLRQRGMGIIYITHRMEELRAIGDRVTILRDGCTVA
                     NGALAALSMEQMIHYMVGREIAALYERTPLEPGEELLRVERPGVCFRIRAREIVGLAG
                     LMGAGRTELCRQIFGLDGDGEVYLFERRVRIRSPRDAVAGGLAFLTEDRQRTGLALRL
                     PIRTNVTLTNLSALSRLGVLTAGREQGPVGNLIQRLRIRADSPEQLAGRLSGGNQQKV
                     VLAKWLFRQARIFLFDEPTRGIDVGAKAEVFRLMDELARAGAGLLMVSSELPELLQAA
                     DRILVMRAGQIIKELARPTTQEEILRYAAVGE"
     gene            complement(18250..19227)
                     /locus_tag="VFA54_03015"
     CDS             complement(18250..19227)
                     /locus_tag="VFA54_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZS49804.1"
                     /translation="MSSEAKVGLFVIISSLILMVAFVSIANVQLRGGLVRYKTYFSFA
                     GGIEKGTVVRFGGLKTGAVADVHPWTEDPTKIEVLLDLKPGTPVRQDSVAAISTLGMI
                     GENYVEITPGKKDSPPLPPGSTIPSQEAVDFATLTRRIGAMTESSEALIIDLHRNLNE
                     ISAKADQLIANLNELTGEKNRQNVEALLERSNKLVADQAPKIDTLTGSLNAAVQKIDA
                     LVADLRGTNARIGDLVSNVNKTVDETRDPIKDDLKQLQNTIAQTQDLIEQLRGTMAYN
                     DENINRMIENFRVSSQNLKEFTAEVKQRPFSLFRIRPKPDRRVPVQGQR"
     gene            complement(19451..20242)
                     /locus_tag="VFA54_03020"
     CDS             complement(19451..20242)
                     /locus_tag="VFA54_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS49805.1"
                     /translation="MPDSPMISVRDLLVAYGNRTILHGISLDVMPGETMVILGGSGSG
                     KSTLLRHIIGLERPQSGQIFIKGVDIVHCSERELNEVRKSMGVSFQGSALFNSMTVAD
                     NVALPLREHTKLAESTIKLMTRIKLEQVGLASCEDYYPAQLSGGMKKRAAVARALAMD
                     PEILLFDEPSAGLDPIIAAGIDELILELKRAFPHMSIVVVTHELASAFLIADRIAVVD
                     KGRILIAGTKEEVRSSSNARVRQFLDRVPDPVNADQEAFLERLIE"
     gene            complement(20504..21295)
                     /locus_tag="VFA54_03025"
     CDS             complement(20504..21295)
                     /locus_tag="VFA54_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS49806.1"
                     /translation="MTLAQIGRSSIQAVEYVGGLSIQFGRGLAAAARLTPFRTRLRWR
                     NTIRQMEIVGVGALPVVCLIAGFVGLIMALQGAYELKKFGALSYVVSLVGVSLTRELG
                     PLMTAIIVIGRSGSAFAAEIGTMTVTEEVDALRTMALEPVDFLLAPKLVAMMIMMPCL
                     TIAADTAGILGGAVFTYASLGMGLRVYLSTTAEVLLLRDIITGLIKSLAFGMIIVQVG
                     CFEGFHVQGGPEGVGRATTRAVVQAIFLVVLADLVFTAFFYLFWS"
     gene            complement(21292..21636)
                     /locus_tag="VFA54_03030"
     CDS             complement(21292..21636)
                     /locus_tag="VFA54_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZS49807.1"
                     /translation="MAKDLEIDIARNGACSVVHVKGDIDLKTSPELRAAILDLFEKRG
                     QERVILDLAAVPYIDSSGVASLVEGLQEAKKRNARFILSGLNEAPRHVLDLTRLTSVF
                     EITQTVDEALKA"
     gene            complement(21640..22080)
                     /locus_tag="VFA54_03035"
     CDS             complement(21640..22080)
                     /locus_tag="VFA54_03035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS49808.1"
                     /translation="MRQAPHQIELKVSSDPRWLRLVRAMMQEVSRQAGFGDAERCDIT
                     IAVDEALSNVIKHSYKGDYGRPVWLTCATEGDRLSIEIRDVGDAPDPKRLEPLPPEQV
                     RPGGRGIFLMRSTMDEVQFERNGDTNLVRLSKYRKTRSPLQYSD"
     gene            22123..23430
                     /locus_tag="VFA54_03040"
     CDS             22123..23430
                     /locus_tag="VFA54_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZS49809.1"
                     /translation="MPKAVSAARWAAYQVLRRVEQERSFAGDLLHSSLTSALDERDAA
                     LAEELALGVLRWRGELDRIISEAGGRRPETLDLEVRIALRLGVYQLLRLDRIPARAAV
                     SESVEIVKRARKSSAAGLVNAVLRNAASHVRPGALPQAAQAAPAWLLERWRRRFGDSL
                     AEKLALQTLNAPPVFLRLNARFDTEETLRLLAAEGVATERAELPYCCKVLAGRPARTE
                     CFRQGRIRLQDLGSQRIVPLLALEPQHRFLDLCAAPGGKTFQALEQRMPYRASGVHAP
                     DFANHIAPAGTGPEGLAVACDRFLERLLTMRRLATLPVAMVALDAACELPFRTKFDRI
                     LVDAPCSGTGTLARNPEIKWKLRPEDIGELAARQKAMLSRALSLLAPAGRLVYSTCSL
                     EPEENEEVVDAVLGPGFQIVETHLWLPGENQGDGFFACVIARR"
     gene            23517..>24118
                     /locus_tag="VFA54_03045"
     CDS             23517..>24118
                     /locus_tag="VFA54_03045"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HZS49810.1"
                     /translation="MLTRRKFVAGGAALALRGGRLRGAAANGGEPDRKNFSGMALELA
                     KKAGAAYADVRINRYQEQGLFTRERQVRSIDSDASYGYGVRVLKKGSWGFAASDDFSE
                     RALAEVVRQAVDIAEADAAISSQPVRLAPEKPHQAKWKSAYTIDPFRVPVEKKIELLL
                     KINETALKVKGARFVASEMRFVREDKFFASSEGSRIEQEIV"
BASE COUNT         4707 a         7279 c         7546 g         4586 t
ORIGIN      
        1 gcgcgggatt ttgagtcccg tgcgtctgcc agttccgcca ctccagcgtg tttcttagta
       61 taacttcgaa gcagcgcctc gggaaaccag gagcgcgggc gccggcattc gcgggtggca
      121 ggtctccgcg ccggaggaag cggcgatcat gcggctcacc gctggcgccc aagacccgcg
      181 ccgctttcag tagagtccgg agggtcttcg ccattaggcg agccgctccg gggatgcgca
      241 gcacgcagcc tgcctgcggc atcctggcct atgggccggg ttctttttta ggccgctaac
      301 tgttctatat gataggcccg gcgattcgga ggcaggaggg agtatgcgtg ccatgccgcg
      361 gacccgaagg cagcccgcct gttacgcgag gcgccggcgg ttctgataag ctactctaat
      421 tgcaagaggt tggagcgcga ttatggcaat cagtcaagaa ttgctcgaaa ttctggtttg
      481 cccggtggat aaagaagcac tagagctgaa gccggaccag agcggcctga agtgcaccaa
      541 gtgccaccgc gtctacccga tcaaagacga catcccgatc atgctgatcg acgaggctac
      601 gatcgaagag tgatcgcggg aaatgaaccc gttctttcgc gcctgcatgc cggcgcgcgg
      661 gttgtcgtaa tccgcctgcg atccatgggc gataccgtgc tgacgacgcc cgccctgcgg
      721 cttttgaaga cggcgcgtcc ggatgtgcag attttcgttg tcgtcgagcg cccttgggat
      781 cgcctgctgg aatcgaacga tgacgtggcc ggaactgtca tctgcgaccg cagcgcgcgc
      841 tggaaagccg ccgcggcgat ccgccgggtt cgtcccgagc tttgtttgaa tctgcatggc
      901 ggctcgacca gtgcggctct gacagcgttt tcgggcgcct cgtggagggc cggcttcgcc
      961 cattttccat tccgggcggt ttacaacgtg gcaatcccgc gcgcgcagga agttttgcgc
     1021 cgcactgcca atgccccggt ccacacggcg gagcatctgg cgtcggcggc gttctatctt
     1081 ggagcaccct ccggggaaat ccctgccgcg cgcctgtttg ccgaagcggc tccccggacc
     1141 tcgccctacg cggtactgca tgtcgccgct gcgtatttca caaagcagtg gcccacagcg
     1201 cggtttgtcg agttgggccg ctggttgcga gaaactctgg gcctcgagcc cattctcact
     1261 gccggccccg gccagggcga tctgctggcg ggcgtggaag gtttggaaac ctctgctaac
     1321 ctgtcggtgg gcggcctgat gtcgctgata aaaggggcgc ggctctttgt gggcaacgat
     1381 tccggacctg cgcatgtggc ggccgcgttt ggggtgccgt gcgtggtgat ttttggatcc
     1441 tcaaattcca gcgtctggcg cccctggaag accaggtgcg aagttgtgga aaccgcctgg
     1501 acctgcaagc cgtgccccgg cgaccgctgc tacgcctttg acgagcctcg ctgcattctc
     1561 tcggtggaac tcgaggttgt gcagaaggcg gtgctggcgg taaccggcca gaaacaaccc
     1621 ggcgctttcg cgcctgtaac tgcggcgtcc acattaccgc atgactgatt tccggcggct
     1681 tctaatttac gtccggccct actggctgcg cctggccgtc tcggtgctgt tgatggccgc
     1741 ggtgggcgcc tgccacgcga tgattccgct gctaattcgt cctgttttcg accgcgtgtt
     1801 gaacccgtct tctccggagg cgcccgtcga gttgttccat cctccgggct ttgcgcgagg
     1861 cctgtacctt catgatctga tgccggcttc cgtacacaac gtgtggacca tggtcgccgt
     1921 gtgcatcctg ggagtgttcc tcattaaggg tgcttgcgat tttgcgggca actacctggt
     1981 caactatgtc ggctttgccg ccgtcaccga tctgcgcaac cagctttacg aaagcatcct
     2041 gcggcaatcc acagccttct tccgcagccg acattctggc cggctcatgt cttcgattct
     2101 caacgacacc gataaggtgc aggtggcgct gtcgcacatc ctggccgact ttctccggca
     2161 gagcttcacg attgtcgcac tgctcctggt ggtgctgcgc accgactgga aattagcttc
     2221 ggtaagcctg accgttctgc cttttgttct gattcctacg gcgagaatcg gccgccgcat
     2281 ccgcaaaact tccagttcca cccaagacag cgtggccgag atgaatcaga ttttgcagga
     2341 gaccacagcg ggccactcga tcgtcagggc gttcgggatg gaagctttcg agatccggcg
     2401 ctttcgcgcc agcgcggcgc ggatgttccg gcgcaacctc cgctacgtgc ggcagcaggc
     2461 cgttgcatcg cccatcatcg agatgttcgg ggccatcacg attgtcgggc tgctgagcta
     2521 cgcgcgcctg caaatcaagg cggaaaccat gaccgccggc cagttcacga gtttcgtcat
     2581 cgcgctgctg atgatgtacg agccggtgaa gcgcctcaat ggcatccaca atatttttca
     2641 gcaagctttg ggagcgtcgc aaaaggtctt cgagtacttg ggccacgagc aggaagtaaa
     2701 ggaggcgccg ggcgctccgg cgctgccgcc gttccgccgc tcgattgaat ttgaaaatgt
     2761 gagcttccgc tatccggatt cgccgaacgg cctggtgctc gacggcatca acctgcgggt
     2821 gaacgcgggc gaagttgtgg cgctggtggg tccgagcggc gcgggtaaga ccacgctcgt
     2881 aaatctgctg ccgcgtttct tcgatccgac ttcgggcgcc atccgaatcg atggatgcga
     2941 tatccgcggc gtaacgatcc gcagcctccg ggaacagatc ggtctggtca cccaagagac
     3001 gtttttgttc gacgatacgg tggcaaacaa tatctcttat gggcggcccg acctgccgcg
     3061 cgagctcatc gagcaggccg cgcgcgcggc gctggcggcc gattttatcg ccgatcttcc
     3121 gctcggttat gatacccgga ttggcgaacg cggccagcgg ctgagcggcg gggagcgcca
     3181 gaggatcgcg attgcgcggg caattctcaa gaacgcgccc attttgatcc tggacgaggc
     3241 tacctcgcac ctcgacaccg aatccgaggt gcttgtgcag cgcgcgctcg ccaacttgat
     3301 ggaaggccgg acggtgatcg tgatcgcgca ccggctctcg acgatccggc gcgccgacaa
     3361 gatcgtggtc atcgagaacg gacgcattgc ggaaatcggc cgccatgaag cgcttctcgc
     3421 caacggcggg ccttacaagc gtctgcacga gttgcagttt cagtaaaaag atgcgccgcg
     3481 cagggcggcg ggctcaggaa acgaagtctt tcattgccgc cggcttgtag gcgggacttg
     3541 tggattatgc cgcggagcat gacgggattt gcgcgagttc agaccgagac atccgagttc
     3601 tgccttacgc tcggcattaa aagcgtgaat caccggtcgc tcgatctgca aatccggctg
     3661 ccgccggagc tcgaaccgtt cgaaatggcc gcgcggcagg ccatgaaaaa gaagatcacg
     3721 cgcggatatg tgcaggtcac ggcggccctg gaatcgcttg tgccgccccg ggtgcacatt
     3781 aaccgcgggc tggtggaggc gtatctcagc gcgtatcgcg atgtggcccg ggagtatggc
     3841 ataacagccg agccggatct gaatctgctt ttccgcctgc ccggaattgt cgctttcgtc
     3901 ggcgcggacg cggaaaagag cgggggcttg gaaaaggcgc tgctggaagc cctggagcgg
     3961 gcgctggacg agttgactct ttcgcgcgaa cgggaagctg ccggcatcgt cgaggaaatg
     4021 atgcggcgct cgcgctctat tgaagccggc ctggcccaaa ttttgcggct tcgggaaggg
     4081 ctcacgtcgg tgctggcgga gcgtctcgct caaaaaattt cggagttgat caaaggcgcc
     4141 gcggccgatc cgcagcgggt gctgcaagag gccgccttta tggcggaccg gacggacatc
     4201 agcgaagaag tccagcggtt gcaggcgcat aatcatcaat tacaggaggt tctcgcgccg
     4261 tcttcctcgc cgggcgaagt cggcaagaag ctggattttc tccttcagga gatgaatcgc
     4321 gaggcgaaca cgattctgtc caagacttcc ggaattggcg aaacgggcct tgcgatcacc
     4381 gatctcggcc tcgcgatccg ggccgaaatc gagaagatgc gcgagcaggc gatgaatctc
     4441 gagtagggca gcccgggctt atgtgccggg cgccaggagc agcgagcatc gccgcggcgg
     4501 ctgcgcgctt ccatttttca catgtccatg gtttttataa tctcagcgcc ctctggctcg
     4561 ggcaaatcga ccctggttga gagacttctg gccacggttg aaaatgtgga tttttcgatt
     4621 tcttacacca cccgcccgcc gcgcgggggc gagaaaaacg gggaacatta ccgctttgtc
     4681 gatcgccaaa ccttcgaagc catgcgtgat aaaggggaac tgctggaatg ggcggaggtt
     4741 ttcggaaatt attacggcac cccccgctcg gcactcgacc aggcccgcga ggccggccat
     4801 gacctggtgc tcgacatcga cgtgcaaggt gcggcacaat taaagaagca gcttccggaa
     4861 gctgtgaccg ttttcattct gccgccctcc cggcatgaac tggagaaccg attgcgcagc
     4921 cgctctctgg acgacgaggc ggtgattgcc cggcgtctgc gcgacgcctc caaggagatc
     4981 cggaattacg ccgcatacga ctacattctg gtaaacgacc agattgaccg ggcggcggaa
     5041 aagctgaaag cgatcttttt ggccgagcgc tgccgcaagc agcgggccga agggatcatt
     5101 cagcccattt tgcaaagctt cgaggtagag aagaaagatg actgaaaaca agccggtgca
     5161 gccggaagcc agttccaatg agccggcggc cacaatcccg ggtgatccgg aacaaagcgt
     5221 ctatcgtttc atcatcgtgg cggcgaagcg cgcacgccag ttgcagtcgg gcgcaaggcc
     5281 gaaaatcccg acccagtcgc gcaaaatgac gcgggtggcg attgaggaaa cgcgccgcgg
     5341 cctggtaccc ttcatagatc cggagaatcc gccgcccgcc tccgaagaag agtatccggc
     5401 ggccgatcag taaccgaagc gacccgatga gaattgtcct cggcgtgacg ggcggcatcg
     5461 ccgcatacaa agccgccgaa ctggcgcgag cgctgcaaga gcgcgcacat acagtggaag
     5521 tggtgatgac cgcgcacgcc caggagtttg tgcggccgct gacatttgcc gcgctgacag
     5581 gacagaaagt catcaccggc atgtttggct cttcgacggc cgaagccacg ctcgagagcg
     5641 ccatcgagca catcgcgctt gcgcaacgct gtgaggcgat tgtggttgcg cctgcgtcag
     5701 ccgatatttt ggccaaattt gcgcacggca tcgcagacga ttttctgacc actctttacc
     5761 tggcctatgc cggtccggtg gtgatggcgc ccgctatgaa ctcgaatatg tgggtgcatc
     5821 cggcgacgcg ggccaatgtc gaggagctcc gccggcgcgg cgcgcgcatc gtcgaaccgc
     5881 ccgagggtta tctcgcctgc ggcatgatcg gacccggccg gatgagcccg attgacgaaa
     5941 tcgtggccgc cgtcgaggaa acgttgcact cccaggccac ccggcgcgat ctggagggcc
     6001 aaaccgtatt gatcaccgcg ggacctacgc gggaaccgct cgacccggtg cgttaccttt
     6061 cgaaccgctc cagtgggcga atgggatttg ctttggccga agaagccttg gcccggggcg
     6121 cacgggtgat cgtcgtggcc ggccccacgg cgttgccccc gccgcccggg gccgaagtga
     6181 tccgggtgga aacggccgcc gagatgcggc aagcggtgat ggatcggctg gagagcgcaa
     6241 ccattgtcat aaaggccgcg gccgtcgccg attttcggcc ggcgcatcgc tctcagcaga
     6301 aattgaagaa ggcggctatt ccgccggcca tcgagctgga gcccacgcct gacattttgg
     6361 ccgaaatcgg ccgccggaag ggaaccaggt ttctggtggg cttcgccgcg gaaaccggcg
     6421 acctcaatga agaggccatc cgcaagctaa aagcaaagaa ttgcgatatg gtcgtggcca
     6481 accgtgtggg ccggcgcggc acgggatttg actcggagga gaacgaagct ttgatcgtga
     6541 caaacgaggg gaaggcaatc gagtttcctc gcggctcgaa acggagcctg gcggcccgta
     6601 ttttcgactg catttatcaa ctccgccttg cccatacctg atttgagatg acagccgagc
     6661 agatccggcg gcttttgctc ttttatcaag acctgggatt tacggaactg ttccggcgcg
     6721 atccccccgc cgggcaacgc gagagcccgc cgcaaaaagc cgcagcgaga gaagcgtctg
     6781 aaggcccgcc gccagggatg cccgccagtt tgctcgatca catcgcggcc ggagagcccc
     6841 gcgagcctct ggcgcaaatc gaggccgaac tggccggctg tacccgctgc cggctgcaca
     6901 agggtcgcac gaatcttgtt tttggagtcg gcaatccgca ggcgcagctc gtttttgtcg
     6961 gcgaaggccc aggggcggag gaagatgcgc aaggcatacc gttcgtcggc cgggccgggc
     7021 aattgctgac tcagatgatc gaaaatacgg cggccaggga aggtctaccg ctgaagcggg
     7081 gcgacgtcta catttgcaat gtcgtcaaat gccggccccc ggagaaccgc acgcccgaga
     7141 aggacgaaat ggatacgtgc agcccgtttc tattccggca gctcgagtcc atccgcccca
     7201 aggccatctg tgtgctcggc gggacggcgg ccagagcgct gctgaacact tccacaggca
     7261 tcacccagtt gcgcggaaaa tggcagaatt ggcgcggcat tcctgtcatg cccacctacc
     7321 acccctcgtt cctgctgcgc ggctacaatg ccgcttacaa acgcgaggcg tgggacgatt
     7381 taaaaaaact cctgcactac gtctacgggt gatcgttctc acctccgctc ccgcccgccg
     7441 gcctgcacga ggtccgcgca aagctctttg cgcattgatt tgcgggggcg ctgcgctctg
     7501 cgagttggtg ggacaacgcg cggctcccgg cgctgcaatc cgtatcgccg ccgtatgcta
     7561 cgatgggctc ttgccaggtt ccgggctccg tgcagtggga cgccgcaaac cccgccaggt
     7621 ccggaaggaa gcaacggtag tggttaaccc cgcgtgccgc ggatcacccg gagcttggct
     7681 taaaagtcag cgtgcagccg ccggctccgc cgcctgtcac ttctaaaagg ccgctgtgag
     7741 ctgagcgaag acaggcggcg ctaggcggtt tgcgcctcgc ggcgggggcg gcgctcgccc
     7801 gcggacgctc gctggaagcg gaaagtctct gtgtatcagg ttatcgcccg caaataccgg
     7861 ccgcaaactt tccggcaggt ggttaaccag cagcatgtca agcagaccct ggagaacgct
     7921 gttgcgaaag gccgggtggc tcacggatac atctttgcag gccagcgcgg cactggaaaa
     7981 accacggtgg cccgcatcct cgccaagtgt ttgaactgcg tgaaggggcc gacgattact
     8041 cctgacctgg agtgtcccag ttgccgggag atcgccgctt cgagctcgat ggacgtgatc
     8101 gagatcgatg ccgcctccaa tcggggcatt gacgacgtgc gggagctgcg ggaagccgtg
     8161 cgctaccggc cgtcgcgcga ccggtacaag gtatttatca tcgacgaagc gcaccagatc
     8221 accgacgccg cattcaatgc tctgctgaag acgctcgaag agcctcccga gtgggtggta
     8281 ttcatgcttt gcaccaccga agcgcacaag attccgccca cgatcgtctc gcgctgccag
     8341 aacttcacgt tccagatggt cgattttgac catgtcgttg cgcatctgca atggatctgc
     8401 gagcaggaag gaatcgaagc cgaagctgat gcgctttctg tgattgcgct ggcgggcgac
     8461 ggcagcattc gcgattctct ctccactctc gatcaggcca tcgccagttt tgggaagacg
     8521 ctccacgccg ctgacgttcg ggatttgctc ggcgccgtgt cgagcgaggt tctggacgag
     8581 ttcgcggcgg cgcttcgcga tcagtcgccc gccggcatgc tcgagttggt cgagaagctt
     8641 accgcgcgcg gacagaatcc gcaacacttc tgccgcgatt tgacgcgttt tatccggaat
     8701 ttaatggtcg cccggatgat gggagccgat tcccgcctca tcgccgcttc cgccgacgag
     8761 cgccggcgcc tggacgctct cgcatcacaa ttttccgaag aagatctgac ccgccatctg
     8821 caactggcgc tgaacctgta cagagacctt caacacgccc cgcatccccg ctttcatctc
     8881 gaaatgggcc tgctcaagct ggtttacgcc ggaaagatgg tggacttgga aaaagcgctg
     8941 gctgccgcct cctctgatcc ccagccgccg gctcacgcgc cgggtgatgc tcagggccca
     9001 actcggggaa gctccacaag aagccgctcc gccgcgcctc ctcccgcgcc cggcggagga
     9061 cgaatgcagg ccgcccagcc tgcgggaacg gcagcggttg cgaagcggaa tcccccagcg
     9121 gcatctccgg ctggcgcgga gacggcgacc ggagcgcagc cgggagctac ggcgtctcct
     9181 caagacgact tccggtcgcg ccttctcgaa gctctgcgcg cggagggcca tgagtttacc
     9241 gcggatgccg tcgagcattc cgcaatcgac ctggccggcg gcgacgtcca ttttctggcg
     9301 ccgcccgatc ttcagttctc cctggagtcg gcgtcccagc ttttgaacga gatcagcggc
     9361 aggctgctgg gacggcgcgc gcggatccgt gttacactgg ctgatacaac aattccaccg
     9421 cggaatgttt cggaaggcgc gccaacccgg gcgggtacgc aagaatcggg agcttccggg
     9481 gactatggcc cggccggcga gcgggcgctg gctcacccgc aggtacagaa gtttctggct
     9541 acgttcggag ggcaaatccg ccaggttcgc gatttgaagg agacatgacc gtgaaaatgc
     9601 ccggcaatat gcagtcgatc atgaagcaag cccaaaagat gcaggagcag ctccagcagc
     9661 agattgcggc cattcgcgtc gaggcctcga cgggcggtgg aatggtcacg gtcaggatgg
     9721 acggccagaa gcacgtgctg gaagtcaaaa ttgatccgga agccgtcagc gatgtagaga
     9781 tgctgcaaga catggttctg gccgctttca acgaggcagc caagaaagtg gatgacgaag
     9841 cgcaatccaa gatgggcggc atgctgggag gccttggctt gccgccgggt atgttttagc
     9901 ggcttggatt tcagccgtaa cgatttgttc ccagcaaaat gcgggctcgg attccaagcc
     9961 cgccgcttct tgtgctatga acgatctcgc tgaaccgatg gctcgtttga tcgacgagtt
    10021 taagcgcctg ccgggcatcg gccaaaaatc cgcgcagcgc atggcgtttc acgtgttgcg
    10081 gagcgcgcgc gaagacgcgg agcggctcgc cagcgccctg ctggatgtaa aagaccgcat
    10141 gcggctttgt gcgctgtgca acaacatttc ggatgcggaa acatgttcca tctgttctga
    10201 ttctagtcgc aatcaaaaag ttttatgtgt ggtggaagag ccgcgcaaca ttctcgttat
    10261 cgagaaaaca cgccaatttc aggggcttta ccatgtcttg cacggcagcc tttcgccttt
    10321 gcggggaatc gggcccgagc aactgaaggt gaaaagcttg ctcgagaggc ttcagcggag
    10381 tacgatagag gaggtcataa ttgccacgaa ccccactgtg gagggcgagg cgacggctgt
    10441 ttatctgtct cgcttgttgc gacctctggg agtccgggta acccgcatcg ggatgggtat
    10501 tcccgtgggc agtgatctgg actatgttga cgaagtgaca atgctcaagt cgatcgaagg
    10561 ccggaaggag atttgaacga cctccgggat tccaggagat acatagtatg agcactattg
    10621 ttaacggcgc accacctcag actgacctct tcgagccgga acaggaattg aaggctccgt
    10681 ttccgggcat tccgaccaca tcggacggag ccggcatggt cgtatgggtc gagaccaaca
    10741 tcacgcaggg cgcctgcgct taccctatca cctcatccac gacgatggga ggcggctacc
    10801 aggttgaggt agcgaacgga aagagaaatc tctggggcga agaactggcc ttcatcgagc
    10861 ccgaatcgga acacagcgct gccaccacct gtgaaggctt tgccgtagcc ggcgggcggg
    10921 tgaccaattt tacctcgggt cagggccttg ttctgatgaa ggaggttctg ttcacgatcg
    10981 ccggcaagcg gctgccggta gtctttcaca tcggagcgcg ggcgatcacc tcgcattcgc
    11041 tgaatgttca cgccggtcac gacgacgttt acgccgtcgc cgactgcggc tggggcgtgc
    11101 tgtacgcgcg caacgcccag gaagcgggcg acatggccct gattgcgcgc cgtactgcgg
    11161 aagacaccga gactccgttt ctgaatgtcc aggacggatt cctgaccacc cataccattg
    11221 agaacgtccg gctgcccgag ccggagatga tgaagttgtt cgtcgggcac ccctcgcgcc
    11281 ggctgcgcaa cctgatggat ccggcgattc ccctgatgtc cggcgtagtc cagaaccagg
    11341 acagctacat gaagggcaag atcgcccagc gccgctacta caatcaggtc cttcctacgc
    11401 tgaagaaggt gatgcgcgag ttttacgaga tgaccggccg ccgctacgac gtggtgatgc
    11461 cgtatcgtct aagcgacgcc gagtatgcga ttgtcggctc cggctgcatg atggaaacgg
    11521 ccgaggcggc ggtcgattac attcgcgatc actacgggat caaggtgggg ctgctgcaca
    11581 tcacctgttt ccgtccattc ccgtcagtgg agattgtgca ggctttgaaa cactgtaagg
    11641 ccatttcggt cgtggagcgg ctggatatcc cgatgatgca gtcgaaccca cagctgtgcg
    11701 agatgaaggc ggctttcgct gacgcgatgg ctgaaactcc cggctatccg ctgctgacgc
    11761 acatgccccg gttctacggc gggtcggccg gtttgggcag ccgggatgtg cgcgcgggcg
    11821 acttcatcgc catcgtggag aacatgcgca gcgcgaagcc gaagatgtat tttacggtgg
    11881 gcatcaagca cgagaccgcg cttgaggtaa agcacgatcc cgatgttcgc agcccgggct
    11941 cgttctcgat gcgcggccac tcggtcggcg gatacggatc agtgacgacc aacaaggtta
    12001 tcgcgacgat cgccggcgaa gtctttggca tggatgtgca ggcttatccc aagtacggtt
    12061 cggagaaaaa aggcctgccc acgacgtatt acctgacaat cgcgaaaaag cacatccgcg
    12121 tgcactccga gctggagcac gtggaattca ttccgctcaa tgacgtgaac gctttcaacc
    12181 tcgaaaatcc cctggcgggt ctggccgaca acggaatggt gttcgtgcag agccagaaga
    12241 cagaccccgc ccaagtttgg aacgccgtgc cgccctgggc ccgcaagttc atgcgcgaaa
    12301 aaaacgcccg gctcttcgcc cttgacacgg tgtcgatcgc ccgcgaggtg tcctcgctcg
    12361 ccgatttgca gcagcgcatg caaggcatcg tgctgctggg cgttttcctc cgggtgacgc
    12421 cttttcaggc ggaatccggc atcagcgaag agcagctttt cgcaggggtc gagaaggccc
    12481 tgcgtaaata tttcggcaag cgcggcgagc gcgtcgtgca agacaatctc aaggcggtga
    12541 gccgcggcta ccatgaagtc atcgaagtgc cgcgggaagt tatgctgagc actgcggcaa
    12601 tcgacgacgc caacgcggcg ttgcgcgtca tctagcgcgg aaagccgccg ggtggattcg
    12661 gtttgctgcg acgaaaggga ctcaatatga ccgaaacgat aaccattcca ctggaagcac
    12721 ggatcgaaga ttaccgcgcc aatgtggtgg atgtggccga cttcaacgag cgcgtggtcg
    12781 gcgcttacaa caacgggacg gcggaaaaag ggctggaggc ggatgaccag gtggcgaaaa
    12841 gcatcatccc ggccgggacc ggcgctttgc gcgatttcag ctatatcgct cccgatatac
    12901 ccgtctttat cggtgagaat tgcgtcgggt gcatggactg cgtcaccgag tgccccgata
    12961 cggcgatcct tggcaaggct gtggagccgc agactctcga gcaatatttg gccggaatta
    13021 cggatgaagc cgagcgcaag aagatggccg agcagtgggc catcaccaac aagtactaca
    13081 cggtgctcga aaaaaagggc gtgggcggtg ggaagttcgg aattttcatc gatccgacca
    13141 aatgcaaggg ctgcgccgag tgcgtcgacg cctgcgggga ccacaatgcc ctgaagatga
    13201 tccgcaaaaa cgatgaaaac atgaagtggt acaaggctgc ttttgacttc ttcaaaagcc
    13261 tgccggagac tccttccaaa ttcatcaatg agaaagccct ggccgatatg atgttggccg
    13321 agcgctcgtt gctgtatgtg gggggcgccg ggtcctgcat gggctgcgga gaggctaccg
    13381 cgctgcgcat gatgctggcg gccactggct ttgtgtatgg gcgcgaaaac atcggcattg
    13441 tggcggcgac cggctgcaac accgtctaca cgtccaccta tccttacaac ccctacctag
    13501 tgccatggac aaactcgctg tttgagaacg ctccggccga cgctatgggc gtgcgcgccc
    13561 gctgggacca gcttggctgg aaagaaaagc gcttgtgggt gatcgggggc gacggggcga
    13621 tgctcgatat tggctttcaa tcgctgtcgc gtatgttcgc ctcaggcatg gacattaagg
    13681 tcctcgtcct cgatacgcag gtctattcca acacgggcgg tcaaacctcg accggaagct
    13741 tcaagggcca ggacgcgaaa atgagcttcc acggcaaggt gatccagggc aagaaggaaa
    13801 accgcaagga gatcgccaac atcgccatga tgcacaagga cgtctacgta gctcagacca
    13861 cctgcgccca tccgaatcac ttctataaag ccgtgatgga ggcgaatgcg tatcccgggc
    13921 cggcgatcgt caacgtatat acgacctgcc agccggagca tggagtggcc gacaatatgg
    13981 ccgaacatca agccaagctg gcggctgact cccgcgcatt tccggttttc atctacgacc
    14041 cgcgcaaagg ccccaagttc aaagaccgcc tcagcttagt cgggaacccg aacgtgaagg
    14101 atgactggta cgtgaacccg aagaccggcg agcagattga ctttatttac ttcgcccgca
    14161 ctgagggccg cttcgccaag catttcgaca aggacggcaa tccctcgccg acgcttctgg
    14221 ccgcgcagca ggaccggctg gaaaactggc atttactcca ggatttggcg ggcattaagt
    14281 aagcggcgga aagcgcctct ttggccaggc cggaatcgtt cccaaaacgg cgccggcccg
    14341 cgatcctgct cagagtccca cactcaattc cagttgttcg gcgtaagcaa acagcgcagg
    14401 ggcgccaccg gtgtgcagga acaacactgt ttctgaagga tcgatctggc cgcggcggat
    14461 gtgatcgatc agcgccgcca tcacctttcc agtataaatg gggtcgagaa agacgccttc
    14521 gtgccgcgcc agcatccgca aggcttccag gcagccggcg gtaggaattc catagcccgc
    14581 cccgaagtag ccttggtcgg tcacaaaatc ttccggcccg acccgcccgc cgactcctag
    14641 cagggcggca gtctcgttag cgatacgcac ggcgcgcccg gttttctccg gttctccccc
    14701 gctcacggcg acgccataga ggaggtacgg cgcgctgtac attttcgcgc ccagcaccaa
    14761 agctgcctgc gtgccccggg aaccggccgc gtagtacagg cgcgagggcg cctcgcccat
    14821 cgccaaaagt tgctgcacga tctccagcgt tccggccaca tagccgcacg cccccactgg
    14881 actggatccg ccgacaggaa tcagataagg gcgctcgccg cgtccctcca gagcggccgt
    14941 cacctcggcg acctttgccg cctctccgtc gcctgtggcc ttggcagggt ccggcccggc
    15001 gggcacaaaa tgcacctccg caccgaggat gtaatcgagc agaagattac cctgcggggg
    15061 gccgcagccc ggcttgcgcg tgaggacgag cgctgcgccc aaacccgcca cgcgcgccgc
    15121 ggcggccgtc attcgggcgt ggttggattg ctccgcgccg gtcgtaacca gcacggtagc
    15181 tccggcgcgg agtgcgtcgc ccaccaggaa ttcgagcttg cgagccttgt tcccgcccag
    15241 ggccaggccg gtgaggtcgt ctcgtttgat gagaatgcgc gggcaccgcc gaggaccacc
    15301 caacgcatcc cgcaggcgct tcagctcatg aagcggagtc ggcagcgtgg ctaaaggaaa
    15361 acgaggctgg gattcaatcg gcactcaacc attctgtcgc gattccgttc aacggcacaa
    15421 ccgccggagc tgaatttttt cgagcgcgcg gcagcgccgg ttgcagagct cagccggttt
    15481 tcactaaact gcgggaaaag accatgattg tctacacggg accgatcgac gacggccgcc
    15541 agctaattca attcctgcgc cgcgaggaac taaatctgcg gtcgctgaaa aagttgatcg
    15601 tttcccgcga gaagaccgtc gtgttggatg tcaacggcga tggaatcgaa tttcccgggc
    15661 tgacttacgg gaatcagatc ctcgaagaat tgctccgcga gctcggcgta gttttcacac
    15721 cccaaacctt gcacaatccc tcggcaaccc ccggcggagt gaaggagttt cgactgagcg
    15781 cccgctggac gtggggccac gaccgcgtga tgtaaccgtg ttcccgagcg gccggccctc
    15841 agctcttcac aaaacgccgc tttcggacct catccaacgt cacggccagg atgatgacgg
    15901 cgccgataac agcttgctgg atgtaaggag aaatcccgag caggtcacag ccattggcca
    15961 gcagtcccat gatgaacgcc ccaatgagcg tgccgatcac gctgccttcg ccgccgctaa
    16021 ggctgccgcc tccgatgacc acagcggcga tcacctgcaa ctcgtagcct tgtcctgccg
    16081 tgggctggcc ggtcatcaag cgtgaggcct cgatcatgcc cgccaggccc gtgagcgctc
    16141 cggagatggc gtagactgcg acggtatgga agctcacggg aatgccggca tagacggcgg
    16201 cttcgcggtt cgacccaatc gcgtaggcat atcgtcccag ctttgtgtac tgcaacagga
    16261 agtgcgtgag catggcgcaa aagaccaaga gataaagcac gaagggaagc ccgagcaccg
    16321 ccccctcacc gagaaaagaa aacccgcgag gcagcccgac gaccggcagt cctccggagt
    16381 aaagcagcgc taggccgcga ataatgccca gcgtgcccag cgtgacgatg aacggattga
    16441 ttttgaggcg ctcggtgagc agtccgttca ccagccccca gaaaaagccg gcggcgatcc
    16501 cggccagaat gcctgctgcg atggcgccgt gctgttccat gattttcgtc ccgatcagcc
    16561 cggcgaaggc gaggatagcc cccaccgaga gatcaatgcc gccggccaca atgatcagcg
    16621 tcattcccag ggccatgata ttgatcacag ccgtctggcg aaccacgctg gagagattgg
    16681 tcgcggtcag gaagggcggc gacgaaatgg aaagccccac gaaaagcgcg atcaggctga
    16741 ggaagggaag cagcctctgg atcattcccc caccgccgca taccgaagga tctcttcctg
    16801 cgtggtgggg cgagccagct ctttgataat ctgtccggcg cgcatgacga ggatgcgatc
    16861 ggctgcctgc aacaattcgg gaagctccga gcttaccatg agcaggccgg ctccggcgcg
    16921 ggccagctca tccatcaggc ggaagacctc cgccttggcg ccgacatcga tgccccgcgt
    16981 gggttcgtcg aacaagaaaa tgcgcgcctg gcggaacagc catttggcca ggacgacttt
    17041 ctgctgattt ccgccgctca agcggccggc taattgctcg ggggagtcag cgcgaattcg
    17101 caatctttgg atcagattcc caaccgggcc ctgctcgcgg ccggcggtga ggacgcccag
    17161 gcgggagagc gctgaaagat tggtcagcgt gacattggtg cgaatcggca gccgcagggc
    17221 aaggcccgtc cgctggcgat cttcggtgag aaaggccagg ccgccggcta cggcatcgcg
    17281 cggtgagcgg atgcgcacgc gccgctcgaa aagatagacc tcgccgtccc catccaggcc
    17341 gaaaatctgg cggcacagct ctgtccggcc cgcgcccatc aggccggcca gccccacaat
    17401 ttcccgggcg cgaatgcgga agcacacgcc cgggcgctcg acgcgcagca gctcctcccc
    17461 cggctcgagc ggcgttcttt cgtagagcgc cgcaatctcc cggccgacca tgtagtgaat
    17521 catctgttcc atcgaaagcg ccgccagcgc gccgtttgcc acggtgcagc catcgcggag
    17581 tatggtgacg cgatcgccga tcgcccgcag ctcttccatg cggtgagtga tgtagataat
    17641 ccccatgccg cgctggcgca ggtttcgtac gatgcggaag acctccgcgg tttcgctttc
    17701 cgaaagggaa gacgtgggct cgtcgaagat gagcaagctg gaagccgagt ggatcgcccg
    17761 gcagatttcg acgagctgtt tttgggccgg gctgagccgc tccacccgcc agcgggactg
    17821 gagggggaag cggtgctctg tgatgagagc gtccgcctcg cgctccatgc gccggcggtc
    17881 cagcattccg cggcgcaggg gctctctgcc cagaaatatg ttttcggcga ccgtgagatg
    17941 cggcgcaatc agcgactcct ggtgcaccat ggccacgccg atcgcggccg cctcggcggg
    18001 agtgcgcagc cgcacgggct cgccgcgcca gcgcattacg ccgccgtcgg gctgcaacat
    18061 gccggcggcg attttcatca gagtggattt ccccgcgccg ttctccccaa gcaaaagatg
    18121 aacctcgccg ggcctgacgc tcaggttgcc gtcttcgagc gcccgcactc cgccgaagcg
    18181 cttcaggatg tgttcgagtt ccaacagcac aaatgtttag atcgtattat tacttggcct
    18241 cgcaccggct cagcgctgtc cctgcaccgg aacccgtctg tccggcttgg gccggatgcg
    18301 gaacaggctg aacggccgtt gcttgacctc ggcagtgaat tccttgaggt tttgcgagct
    18361 gacgcgaaaa ttctcgatca ttcgattgat attttcgtcg ttatatgcca tggttccgcg
    18421 cagttgctca ataaggtcct gcgtctgcgc gatagtattc tgcaactgct tcaaatcgtc
    18481 cttgatcggg tcgcgcgtct catcaacggt tttgtttacg ttgcttacga gatcgccgat
    18541 gcgggcattg gtgccgcgca ggtcggcgac aagggcgtcg atcttctgaa cggcggcgtt
    18601 gaggctgccg gtgagcgtgt cgatcttggg cgcctggtcg gctaccagct tgttgctgcg
    18661 ttcgagcagc gcttcgacat tttgccggtt cttttctccg gtcagctcgt tcaaattggc
    18721 gatcaactgg tcggccttgg cggaaatttc gttcagattc ctgtgcagat cgataatcaa
    18781 ggcttccgag ctttccgtca ttgccccgat gcggcgggtg agagtggcga aatccaccgc
    18841 ctcttgcgaa ggaattgtgc ttccgggcgg aagcggcggc gaatccttct tgccgggggt
    18901 gatctccacg taattttctc cgatcatgcc gagtgtgctg atggcggcga cggagtcctg
    18961 acgaacgggc gtgccgggtt tgagatcgag caacacctcg atcttcgtgg gatcctcggt
    19021 ccacggatgg acgtcggcca ctgcgccagt cttgagcccg ccaaaacgaa ccacggttcc
    19081 tttttcgatg ccgccggcaa aagagaaata cgtcttatag cggaccagac ctccgcgcaa
    19141 ctgcacgttg gcgatggaaa cgaaggccac cataagaatc agcgacgaga tgatgacgaa
    19201 aaggccgact ttggcttcgc tggacattga ttatcagcta tcagggtcat ccgctgtcag
    19261 cggcgggctt tcagcgccgg ggatacccgc ccaacggcca tccccggatc gccggcctcg
    19321 ctttcaatcg tcagacgggt gtttcccgct gataaccagg tccggcggcc ggttaggcgc
    19381 atggctaaga gcctatgttc tgatgctagc cgctcgctgc tgattgccga gagcgcctaa
    19441 gccccgacgg ttattctatc agcctttcca agaaggcctc ctggtcggca tttacaggat
    19501 cgggaacgcg gtccagaaac tggcgcaccc gcgcgttgct cgaagagcgg acttcctctt
    19561 ttgtgccggc tatcagaatg cgtcccttgt caacgaccgc gatacgatct gcgatcagaa
    19621 aagcgctcgc cagctcgtgc gtgaccacga cgatggacat gtgcgggaag gcgcgtttca
    19681 gctcgagaat caactcgtcg atgcccgcgg cgatgatggg atcgagcccg gcggaaggct
    19741 cgtcgaacag cagaatctcg ggatccatcg ccagcgcccg ggccaccgcg gcgcgctttt
    19801 tcatcccgcc cgagagctgc gccgggtagt agtcctcgca cgaagccagc cccacctgct
    19861 ccagcttgat acgcgtcatc agcttgatgg tggattccgc cagcttggtg tgctcgcgca
    19921 aggggagggc gacattgtcg gcgactgtca tggaattgaa gagcgccgag ccttggaagg
    19981 agacgcccat cgacttgcgc acctcgttga gctcgcgctc ggagcagtgc acaatatcta
    20041 cgcccttgat gaaaatctgg ccggactgcg ggcgttccag accgatgatg tgccgcagca
    20101 gcgtgctttt gcccgacccg ctgccgccta gaatgaccat ggtctccccc ggcatcacgt
    20161 ccaggctgat gccgtgcagg atcgtgcgat tgccgtaggc cacgagcagg tcccgaaccg
    20221 aaatcatcgg agagtcaggc attgggccga agcgatcagc cgccagtcgc ggccggggaa
    20281 gcccggagaa gtcccgggta ccgattgccg gcggcgggcc gccgaaccta ttttacccgc
    20341 ttctttcgat tcacttcgca gggaccttcg ttatcagctt tgagcgattc ggcgtcggct
    20401 atccgctttc ggctacttac gagcatctgt cgagggagcg aacagcaggt ccaccggacg
    20461 cgaacaccgc cggcctgcga gtcgacaccc cggatcgcaa tttctacgac caaaacagat
    20521 agaagaaggc cgtgaaaacc aagtccgcga ggacgaccag gaagatcgcc tgtacgaccg
    20581 ctctggtggt ggcgcgcccg acgccttcgg ggccgccctg cacgtggaag ccctcgaagc
    20641 agccgacctg cacgatgatc atgccgaaag ccagactttt aatcaggccc gtgatgatgt
    20701 cgcgcagcag gaggacctct gccgtagtgg aaaggtaaac acgcaagccc atgcccagac
    20761 tcgcatatgt gaagaccgcg cctccaagta tcccggccgt gtcggcggcg atcgtcaggc
    20821 acggcatcat gatcatcatg gccaccagct tgggggccag aagaaaatcc accggttcca
    20881 gggccatggt gcgcagcgcg tcgacttctt cggtaacggt catggtcccg atctcggcgg
    20941 cgaaggccga gccggaacgg ccgatcacga taatggcagt catcagaggg ccgagctcgc
    21001 gcgtcaggct cacgccgacc aggctcacca cgtagctgag ggcgccaaat tttttaagct
    21061 cgtaggcccc ctgcaaggcc atgatgaggc ccacgaaacc ggcgatgagg cagactacgg
    21121 gcagcgcgcc cacgccgaca atctccatct gccggatcgt gttccgccac cgcagccggg
    21181 tgcggaaggg agtgaggcgg gccgcggcgg cgaggccgcg tccgaattgg atgctgagcc
    21241 cgccgacata ctccacggcc tgaatgctgg agcggccgat ctgggccaac gtcatgcttt
    21301 cagagcctcg tccaccgttt gcgtaatttc gaaaacgctg gtcaggcggg tgagatcgag
    21361 aacgtggcgc ggcgcttcgt tgaggccgga gagaataaaa cgggcgttcc gttttttggc
    21421 ttcctggaga ccctccacga ggctcgctac tccggaactg tcgatgtagg gcaccgccgc
    21481 gagatcgagg atgacgcgct cctggccgcg cttctcaaac aagtcgagaa tcgcggcgcg
    21541 cagctcgggc gacgtcttca ggtcgatgtc tcccttgacg tgcaccaccg aacacgcgcc
    21601 gttgcgggca atgtcgattt ccaggtcctt ggccataagt caatcggaat actgcaaggg
    21661 acttcgcgtc tttctatatt tcgataatcg caccaaattc gtatcgccgt tacgttcaaa
    21721 ttggacttca tccatagtgg accgcatcag aaagatgccg cggccgcccg gacgcacctg
    21781 ttccggaggc agcggctcga gccgttttgg gtccggagcg tcgcccacgt ctctaatctc
    21841 gatcgataac cggtcgcctt cggtggcgca cgtaagccag accgggcgcc cgtaatctcc
    21901 cttgtacgaa tgtttaataa cgttgcttag cgcttcgtcc accgcgatgg tgatgtcgca
    21961 tcgctccgcg tcgccgaagc cggcctggcg cgagacctcc tgcatcatcg ccctcaccag
    22021 gcggagccag cgcgggtcgc tggaaacttt tagttcgatt tggtgcggcg cctgtctcaa
    22081 atctggacgg aaatttcgag gggattataa catggttgtc gcatgccgaa ggctgtctcc
    22141 gccgcgcgct gggccgcata tcaggtgctg cggcgcgtcg agcaggagcg gagctttgcc
    22201 ggcgaccttc tgcactcttc gctcaccagc gcgctggacg agcgcgacgc ggcgctcgcc
    22261 gaggaactgg cgctgggcgt cctgcgctgg cgaggggaat tggatcggat catctcggaa
    22321 gctggcggcc gtcgcccgga gacgctcgat ctcgaagttc gcatcgccct gcgccttggc
    22381 gtatatcaac tgctgcggct cgaccggatc ccggctcggg cggcggtttc cgaaagcgtc
    22441 gaaatcgtca agcgcgcacg caagagctca gcggccggtt tggtcaacgc cgttctgcgg
    22501 aacgcggcct ctcatgtgcg cccgggggcg ctgccgcaag ccgctcaggc cgccccggcc
    22561 tggctgctgg agcgctggcg gcggcgcttt ggagacagcc tggcggagaa gttggccttg
    22621 caaaccctga acgctccgcc ggtgtttctg cgactgaatg ctcgattcga tacagaagag
    22681 accctgcgtc tgcttgctgc cgaaggtgtt gcaacggagc gggcagagtt gccgtattgc
    22741 tgcaaggtgc tggccggcag acctgcccgg acagagtgct tccggcaagg acgcattcgc
    22801 cttcaagact tgggttcgca gcgcatagtc ccgctgcttg cgctggagcc gcaacatcgt
    22861 tttctggatc tctgcgcggc ccctggcggt aaaacctttc aagctctcga gcagcggatg
    22921 ccgtatcggg caagcggcgt ccatgccccg gatttcgcaa atcacatcgc gccggcagga
    22981 acggggcccg aaggcctggc cgtcgcctgc gatcgctttc tggaacgtct cctgaccatg
    23041 cggcgtcttg ccacgcttcc ggttgccatg gttgcgctgg atgcggcctg cgagttgccg
    23101 ttccgcacca agtttgaccg cattctggtc gatgcgccct gctcgggcac ggggacgctg
    23161 gcgcgcaatc cagagatcaa gtggaaactc cggccagagg acatcggcga gctggcggcc
    23221 cgccaaaaag ctatgctctc ccgagcgctg tccctgctgg cgcccgcggg ccggttggtg
    23281 tactcgactt gttcgcttga gccggaggag aacgaggaag tcgttgatgc cgtgctcggg
    23341 cctgggttcc agatcgtaga gacccaccta tggcttccgg gcgagaacca gggggacgga
    23401 ttctttgcgt gcgtgatcgc gcggcgctga cgcggcgctc tgctcagcac cgtgtcgact
    23461 ccgtgtcccg cggccgcaga tggctcgttt tctttctgct acagtagtac gcagccatgt
    23521 tgacccgaag aaaatttgtg gctgggggcg cggccctggc tctgcggggc ggccgcttgc
    23581 ggggagcggc cgcaaacgga ggcgagccgg accggaaaaa tttttccggc atggctttgg
    23641 agcttgccaa aaaggcaggc gccgcttatg ccgacgtccg catcaaccgc taccaggaac
    23701 agggcctttt cacccgtgag cgccaggtgc gcagcatcga ctccgacgcc agctacggct
    23761 acggcgttcg cgtgctcaag aagggaagct ggggttttgc agcttccgac gactttagcg
    23821 agagggctct ggctgaagta gtgcggcagg cggttgatat tgccgaagcc gatgcggcga
    23881 tctcttccca gcccgtccgt ctcgcccccg aaaaaccgca tcaggcgaaa tggaaatcgg
    23941 cctatacgat cgatcctttc cgcgtgccgg tcgaaaaaaa gatcgagctg ctcctgaaaa
    24001 tcaacgagac agctctgaaa gtgaagggcg cgcggttcgt ggcaagcgaa atgcgctttg
    24061 tccgcgagga taaatttttc gcttcgagcg agggctcgcg cattgagcag gaaattgt
//
LOCUS       DASREK010000051        22962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1511220, whole genome shotgun sequence.
ACCESSION   DASREK010000051 DASREK010000000
VERSION     DASREK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 22962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22962
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511220"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="VFA54_03050"
     CDS             complement(<1..1655)
                     /locus_tag="VFA54_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZS49811.1"
                     /translation="MKVYEGKDIRNIGVVGHGDCGKTSLVAGFLYTGGATNRLTRVEE
                     GNTVTDFDEEEQARKITISSAVAFLEWNKTKLNFIDTPGYNIFIHDAKSALIAADSAL
                     VVVDAVSGVEVQTEKVWAYADEYQLPRAIVVNKLDRERASFERSLENIHEIFGRTAVP
                     VQLPIGAERDFKGVVDLVSLKAYAYTTDGDGKAKEEAIPADLSEAAQKAHEALIEMIA
                     EGNDALMEEFFDKGTLSPEHIVEGLRDAVRQRRIVPILCASAIRNMGSDLILNFLAEN
                     MPAPADRGEMQGRSPDGAPVSRKISDAEPLSLFVFKTVADPFAGRVTYFKVISGVLKN
                     DANVYNYTRGAMERFSHIGILFGKTVTPVTEIHAGDIGAVAKLKETLTGDSLGDKGSP
                     ILYPTVVLPVPAIAYALEPKSRQDEDRMSAAIHRILEEDQSLRFYRDPQTKEFLLAGS
                     GQQHVEVIVSRLKKRYGVEVTLKAPKIPYRETIRGRADVQGRHKKQTGGHGQFGDCKI
                     KVEPLPRGANFEFVNDIFGGAIPKTFIPAVEKGILEAAERGYLA"
     gene            1870..3348
                     /gene="gatB"
                     /locus_tag="VFA54_03055"
     CDS             1870..3348
                     /gene="gatB"
                     /locus_tag="VFA54_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZS49812.1"
                     /translation="MSVVEVALPGPETIARYEPVIGLEVHVQLMTRTKLFCGCSTEFG
                     SPPNTQVCPVCLGLPGALPVINREAVALAVRAALALNCEVRSSSRFARKNYFYPDLPK
                     GYQISQYDKPLAEHGWLDVPLDGGFKRVGVTRVHLEDDAGKSIHDGYKDSDRYSYVDL
                     NRSGVPLIEIVSEPDIRSSAEAYAYLTELKQIMQYISVSTCDMEKGHLRCDANVSVRL
                     RGAAKFGTKAEVKNLNSFRYLQKALDYEISRQVGVLESGGAILQETRLFNVETGETVG
                     MRSKEHAHDYRYFPEPDLLPLVVSESWREQIRASMPELPAAKRKRFVEQYGIPDYDAQ
                     VLTLAPALADYFEEVARQSGEPKAASNWVMGELTGALKAAGKPIEESAVSAANLAELV
                     RLIAAGEISGKLAKDIFAKMFSTGRSASEIIEAEGLKQISDTGELEKMVDDILAANPK
                     QVEQYKNGKKGVLGFFVGQVMKATRGQANPAVVNEILGRKLG"
     gene            3320..4057
                     /locus_tag="VFA54_03060"
     CDS             3320..4057
                     /locus_tag="VFA54_03060"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZS49813.1"
                     /translation="MKSSAGSWDRASPLALSAYSLRLGKILPRGFYERPVEQVARDLL
                     GNFLLHKIGNTTLAGKIVETEAYLGVNDRAAHAWRGVTHRTRVLFGPPGHAYVYFIYG
                     MYECLNLVAEPEGRAGCVLVRAVEPVLGIGEMRRRRPGARRLEDLASGPGKLTLAMGI
                     TRKHNGADVTRGPLVVREPAIREAFRIAAGPRVGITHNADWPLRFYIEGSPYVSRPGR
                     LPAAEPANEQTASHGANRKRICPGETV"
     gene            4065..5048
                     /locus_tag="VFA54_03065"
     CDS             4065..5048
                     /locus_tag="VFA54_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS49814.1"
                     /translation="MAAQAKQPLTLDVLDLRHFQSPDFDALLGEEAQIWADKLHWDFR
                     PSCDIVRRYLDLHALSGYALIEGRQPVGYSYYVHEDHKVLIGDLFISEPYRTLEAQHQ
                     LLRHVVETAQNTPGVRRIEGQLMMLESASVGSLYPAAQLSIFERNFMLAQGISTLRRE
                     VFGAVPPGSIAFERWSEQQMEQAANLIARAYRTHVDSEINDQYRSVSGARRFLHNIVQ
                     YPGCGAFYAPAAVMALDRKTRELCGISLTSLVRPDVGHITQICVAPEWRGLGIGSELL
                     WRSIQAFREAGCAEVSLTVTAANTGAVRLYERVGFRTVRKFRAYVWENFRK"
     gene            complement(5478..6968)
                     /locus_tag="VFA54_03070"
     CDS             complement(5478..6968)
                     /locus_tag="VFA54_03070"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HZS49815.1"
                     /translation="MKLTLNSQPKETIATEALAVVVFESANGAPADEPFRRLDAQTGG
                     LLSELYASGETTGKALDTVLLHHPTGLAAKRLLLVGGGKVEKFDAAQLRKAAGTALRY
                     LKGRGVHEFVFAPSGGFSPEAQVAAAAEGMLAADFEPDSYKTDKKDDKRIDQASVAVS
                     DNNGPKLEAALRRAVIAGECQNFARGLVNEPANRLAPKDLAARAQQMAAEKGLEIEIL
                     DEQRMRALGMGALLGVAQGSAEPPALILLRYRPSSPPSSDVHLGLVGKAVTFDTGGIS
                     IKPAEGMEKMKYDMAGGAAMIAVMRALADLKPSVPVSAFIPSVENMPGSRAQRPGDVQ
                     TTMSGKTVEVINTDAEGRLILADAMTYARQQGCTHLVDAATLTGAIVVALGHYNVGAF
                     TNDQTWLERLLASARECGEKMWPMPLDDDYKEQLKSIIADLPNVGTRWGGAITAAMFL
                     KEFADPTPWVHLDIAGTAWLEEAKPYMAKGPSGIAVRTLIDLAMKM"
     gene            complement(6971..7444)
                     /gene="smpB"
                     /locus_tag="VFA54_03075"
     CDS             complement(6971..7444)
                     /gene="smpB"
                     /locus_tag="VFA54_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZS49816.1"
                     /translation="MANPATNEERVLSANRRAAHDYFLLERFEAGLALTGTEVKAARE
                     GKVQLKDSYVSIEAGEAWLRNAHISHYSHGNRENHDPVRARKLLLHRQEIQKLIGKTR
                     EKGLALIPLRFYLKKGRIKCELALAKGKHLHDKREAERARTAEAEAREAMRRRSL"
     gene            7538..10183
                     /locus_tag="VFA54_03080"
     CDS             7538..10183
                     /locus_tag="VFA54_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS49817.1"
                     /translation="MTDFNIKRASRFAALCLSLWLAGGGLVPLAAAPKGNAIFKQGRK
                     AELSKDYDKALELYERALREDPENQTYMLATRRMRFVAGQTHVDQGHRLLDQGKLVEA
                     AAEFEKANTIDPSLTIAQQELKRTLDLIAKREQEQKQKEGQNQPGGGAGAAQPAKPGG
                     AGAAEAQKPEPGLSPLLEAKKETEELLESAQPVPTLRPLSTQPINFKASNNSKVIFET
                     IGKLAGINVLFDPEMQGRRITVEINNATLLQALDYVATISKAYWKPLSANAIFVTDDN
                     TTKRRDYEDYVVRTFYLSNVYTAQELQEIATAVRSVTEIRRVFTINSLNALVIRGTAD
                     QLALAEKVIDDVDKSRGEVIIDVLVLETSRTTARDLGLTPVSGTTPGINVSVAPVSGS
                     VTTGTSTGTTGTTTTGTTTTGTTTPTTGTAGTTTTPAAGTYTFGHLQGNSFSLSLPSA
                     NLKALMSASDTRILQSPRVRGFDGYKTSLRIGQRIPIATGSFQPGIGGVGINPLVNTQ
                     FNYQDVGVNVDLTPRVHYGREVSMHIEIEISTVADHVDIGGISQPVIGQRKIIHDLRL
                     KEGESSIIGGLNQTQVTRTVAGVPGLMQIPVLGRLFSNEHKEVSDQEILIVLTPHVVR
                     VPELTDLNMKGISAGTDQIVRLNFAPPPNDGPALPPLPPPGGTAPAGAPAPATPGAAP
                     AAPAVPTAPAAPAGAPAQPTVPPPVPGAAIAPTPPPGPRGPGPRPVTPGAAQPAAPRG
                     AEAAAPRLRFESAQVSGAMGTRITLNLLAENVTDLFAAPVRVTFNPKQLSLVDVRRGG
                     LLASDGQDIIFSRNIQNDSGEASINLSRFPGSGGVSGSGSLLTLEFQPVAAGSSTVSV
                     TGAGVRNARQEAITLGGAQAEVVVR"
     gene            10204..10740
                     /locus_tag="VFA54_03085"
     CDS             10204..10740
                     /locus_tag="VFA54_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZS49818.1"
                     /translation="MSGRLAARAGAERRTVGRTGPPGKKAARPESGLTLIELIVAFTI
                     MLILTTMAVPLARVKVRREKERELRYALREIRSAIDRYKDASDRGLLGPPKLESEGYP
                     PDLQTMVDGVKMQGTVDKKIRFLRRIPIDPMTGTADWGLRSMQDDPTSQSWGGQNVFD
                     VYSKSMDTALDGTKYSDW"
     gene            10849..11247
                     /locus_tag="VFA54_03090"
     CDS             10849..11247
                     /locus_tag="VFA54_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZS49819.1"
                     /translation="MKNPRSRAGMTLIELMIVLTIIITVISVAVPLYQKAIIRSKESV
                     LKQNLFTMRTLIDEYTFDKEKAPQSLEDLVTEGYLRELPMDPFTKKRDTWKVVMEDTL
                     QSVDQKEPGIFDVHSGYDGTALDGTKYSDW"
     gene            complement(11298..12092)
                     /locus_tag="VFA54_03095"
     CDS             complement(11298..12092)
                     /locus_tag="VFA54_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49820.1"
                     /translation="MWRRKWALAFLCLGPAALYGQGNYEVQVYPYETVEPGRTMVESH
                     MNFTLEGSKTTDDGTRPTNHQYHETIEITHGFTPWFETGFYIFTSVQSGYGWSWVGDH
                     IRPRFRVPESWHLPVGLSLSQEFGYQRAVYSPDTWTWEIRPIIDKEINRWYFSFNPTF
                     DRAFHGPSVSKGFEFSPNFKLSYDVWVKGEGEQAKKVQAGFEYYGALGPVTGFDPLRE
                     QQQQILPAVDLVLSPNWEFNFGVGVGTTAATDHLLIKMILGYRFKH"
     gene            12230..13594
                     /locus_tag="VFA54_03100"
     CDS             12230..13594
                     /locus_tag="VFA54_03100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZS49821.1"
                     /translation="MKKFPGVCLLAAALAWGSPGPSANIFPFHYSIDDLPNGLRLITV
                     PTGLPNIVAVYTVVRTGSRNEVEPGKSGFAHLFEHLAFRGTEKYPADKWEAVMQAAGA
                     QTNAYTTDDRTVYHEVFAKEGLESVLAAEGDRFQNLKYSEQDFKTETRAVLGEYNKNS
                     ASPFEKLDEVVQATAFTRHTYRHTTMGFVQDVENMPNEYSYGLEFFRRWYRPENTVIC
                     VVGDVDRRTTLALVKKYFGGWKRGSYKAEIPAEPPQTEPRSAHVDWPAPTLPLVTVAF
                     KAPAYDDETKDSAALDIILFQAFSQNSDLYRKLVINEQKVDLLSADNPDHIDPFLFEV
                     TARVKDPKDVDYVQQQILQTCRDLQEKPLPRQRLDEVKSHLIYSFALGMNSTARVAET
                     LAAYVGLRRTPETINKRYALYRSITPEDVQAAAKKYFVPQGRTIVTLSHKAEGRTSGT
                     GEAR"
     gene            13591..15111
                     /locus_tag="VFA54_03105"
     CDS             13591..15111
                     /locus_tag="VFA54_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZS49822.1"
                     /translation="MNRQRRFRASLGRFGLFVLAAIALSHAAWAAQQIETVALPSDSP
                     LVSFRILFKTGAASDPAGKEGIAALTAAMLAQGGSRDRSYEQILSALFPMASAVRSQV
                     DKEMTVFSGTTHIDNLEKYYALIRSMLLDPGWRPDDLRRLRTDTINRLRVELRSNNEE
                     ELGKEALYLRIYGPAHPYGHENLGAVSSLEKLSLDDLQAFYRQHYTRANLVIGLAGGF
                     PKDFLEKVRADFSRLPEGAQSTPSDLPQPEPASKLRVLLIEKNTRATAISLGFPLRVN
                     RSSKDWPALFLAQTYFGAHRSSNGVLFHDLREVRGLNYGDYAYIEYFPRGMFQFEPDP
                     NLARRQQIFQIWIRPVEPQNGMFALRAALYELDKLVKEGLSKEKFEATRSYLSKFVNL
                     LTQTPSQQLGYAIDSKYYGLPDFNGYVKNALARLTLEEVNAAIRRNLRSSDLDIVIIT
                     KDAQGMKKAILSGEPSAISYVSPKPPEVAAEDKIIGSYKLDVSSVDIVPAESLFEN"
     gene            15358..15744
                     /locus_tag="VFA54_03110"
     CDS             15358..15744
                     /locus_tag="VFA54_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HZS49823.1"
                     /translation="MLQPGVTHQETMVVTEAEAIHFMGSGIRALSTPSMILWLEITAR
                     NAVLPLLEPGQDTVGTLVNVAHLAATPLGMKVTCRATLNAIDRRRLTFAVEAYDEKEK
                     IGEGTHERFVIDVQRYAERLSAKMRA"
     gene            complement(15882..16934)
                     /locus_tag="VFA54_03115"
     CDS             complement(15882..16934)
                     /locus_tag="VFA54_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZS49824.1"
                     /translation="MKCANCGMQISAGEKFCQHCGAPVASRAAAAPSGATDAPRGSSG
                     SRWLWIAALLVLVATGAALLYRLLSLRRANPGSVELTVATAPAGEQRPPAGESANRLE
                     ESPPSVPARGGESGASRRDGEPASTHRKEASGKEAKLAPDVGDAAASRLPKNQAAVQS
                     AQAARSPAPDSVANSAATSSEPAAGAPTAAPPASEASPPPSRPSRPRIHLLKPDPSET
                     PPAPAAAPSASQPGPSYGSDAAASRLTLHRAPAAPGEPAAAPEPVPYPGPSAGTLIWS
                     GQVEKDTLIRIEGDRVSPGRLTGELPGVPVTIDVPSDSLAIVEGPSEANHYKRLYLRS
                     LKRLRSAVVIKWTVRQ"
     gene            complement(16984..18243)
                     /gene="dgoD"
                     /locus_tag="VFA54_03120"
     CDS             complement(16984..18243)
                     /gene="dgoD"
                     /locus_tag="VFA54_03120"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF010624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HZS49825.1"
                     /translation="MKRREFLAGSGVPLLAAAMPQAGSQRAGLSSDPAGGRPAIKITD
                     IKTYMVGVGGRNYIFVKVLTDQGIHGIGEAYSVGPDEATVAAINDFKSWIVGMDPRNV
                     EYLWATMYNFTRFPGGSVVNAAISGIEHALWDISGKAAGLPVYMLLGGKIRDKVRVYQ
                     SPGGRDARELGANAKALVQKYGYTAVKIGPHPPGGNAMPYNAVTRAAGQRISAVRDAV
                     GPDVDIAVDAHAVFFEPIRAIRMAQRLEPYQPFWLEEPLRPENVAAYSKLAAHVNIPL
                     ATGEENYTKHEFRPILAAQCVDIIQPDVTVCGGLLEMKKIAAMAEAHSVMVAPHNPVG
                     PLATVVNVHFAASTPNFLILEYHPDDQAPRKDLLQEPVMVKNGYIPLPTKPGFGVELN
                     EEAFPHYPPKPWRRAFQYRADGSPAFI"
     gene            complement(18291..18908)
                     /locus_tag="VFA54_03125"
     CDS             complement(18291..18908)
                     /locus_tag="VFA54_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49826.1"
                     /translation="MNTAGSSDLTPPAAATGASTAAAVPAEPVQPPAAAEVEIPAGTE
                     LQVRLAQPLGSAGSAAGEKFEATLNQPVVLGDRVVVPKGAVVRGHVVSAVASGRLRTP
                     ARLSITLDTLAWQDQEYSIKTTRVSRSAKSHKKRNLVLIGGGSGAGALIGGLAGGGTG
                     ALIGAAAGVGAGTAGAYATGKKDVLLPAEALLTFRLQAPVKIRKG"
     gene            complement(19332..20645)
                     /locus_tag="VFA54_03130"
     CDS             complement(19332..20645)
                     /locus_tag="VFA54_03130"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZS49827.1"
                     /translation="MPVINVGIVGLGNVGSGTLTILHENARQIEAKLGFPLAVKALCS
                     RSIRDRPPAAARLFPDALHTADWRDVVSHPEIHIVAELVGGSGVAAEIVCASIRHGKS
                     VVTANKELMAAEGPRIWDEAIRAGICLAMEASVAGGIPIHAVLREGISGDRVEALFGI
                     LNGTCNFILTEMEKRREPLPAILAEAQRLGYAEADPSADIDGYDARSKLAILAALAYG
                     VRVSPADIYVQGIRRISPMDFSYAHRLGHTIRLIGAARQTPEGLLLSVRPALIPLSAI
                     MAHVDGAYNAIWVKGAHGADTFYYGKGAGSLPTGVAVVSDLMRVAREIVNGSPERVSP
                     FAHDRLREYAPIPITHQVRPYYLRFRVIDRPGILASLAAILASAHISLEAVLQEPCEN
                     KQDLPFVITLEPTSERAVNEAVAKMSHLDFLLEPPLAMPMEPALG"
     gene            20690..21022
                     /locus_tag="VFA54_03135"
     CDS             20690..21022
                     /locus_tag="VFA54_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49828.1"
                     /translation="MTLPIGTSVLLLLLLGAPQRPYPPERTGPAGAEGRPDVLLPSGK
                     SQRQEILKADFAKTKADAAQLLDLAQQLKQELDRDDHNVLDLRAMKNAEEIEKLAKRI
                     KDRMKQHL"
     gene            21107..21874
                     /locus_tag="VFA54_03140"
     CDS             21107..21874
                     /locus_tag="VFA54_03140"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS49829.1"
                     /translation="MKTLLTMRARVLALALITALPGANTGCNSLKSVQAQPAKNKETL
                     DLGKVTRKRQIAPVCSDAEWLTRPERDATEQPEKVLDALEIPEGATVADVGAGVGYFT
                     WRLARRVGPKGKVIAEDVQQHMLDLLAENLKQRRITNVEMVLGGVRDPHLPENAVDLA
                     LLVDVYHEFAEPEAMIHHIRQALKPDGRLVLVEYRKEDPSIPILPLHKMTVEEVRSEI
                     EPLGFQLQKTLEFLPTQHIIIFTKKPLPSEERAAASR"
     gene            complement(21866..>22962)
                     /locus_tag="VFA54_03145"
     CDS             complement(21866..>22962)
                     /locus_tag="VFA54_03145"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZS49830.1"
                     /translation="YFYGIQIHDVFGSTAVHVQWLSFPLTLLWLVGCANAFNLIDGMD
                     GLASGVGFFATCTMLVASMLQSSGLVVLGLLTAPLAGALLAFLRFNFNPASMFLGDSG
                     SLTIGFLLGAYGLLWSQKSITLLGMTAPLMALAFPIMEAGISIARRFLRGDSIFSADR
                     GHIHHRLVDLGLTPRRAVLLIYAITSVCAMISLLAAMPQFRYGGLVIILFCAVSWMGV
                     QHLGYAEFSEARKIILSGTVRQLIASQTKLRSYRYALERAESIDECWRLTVDAMRELG
                     FDAVELGLPGGVRLHVWLHPGGEDRCSDRCWTMRVPFGPSVDDGWLQLSRDMERGEGY
                     LMLHGVVSTVRDLLPRKLLECAPPVPADSA"
BASE COUNT         4625 a         6979 c         7139 g         4219 t
ORIGIN      
        1 gccagatagc ctcgctcggc cgcttccaaa atcccttttt ccacagcggg aataaaggtt
       61 ttgggaatgg cgccgccgaa gatgtcattg acaaattcaa aattcgcgcc gcggggcaga
      121 ggctccacct tgatcttgca atcgccgaac tgcccgtggc cgccggtctg ctttttatgc
      181 cggccttgca cgtccgcacg gccgcgaatg gtctcgcgat agggaatttt gggtgctttt
      241 agcgtgacct cgacgccata tcgcttcttc agccggctga cgatgacctc cacatgctgc
      301 tgccccgatc ccgccaggag aaattccttc gtctgggggt cgcgatagaa gcgcaaagac
      361 tgatcttctt ccagaatccg gtggatggcg gcgctcatgc ggtcctcgtc ctgccgcgat
      421 ttgggttcga gcgcgtacgc gatggcggga accggcagca cgacggtggg atacaggatc
      481 ggcgaaccct tgtcgccgag cgaatcgccc gtcagcgttt ccttcagctt ggctaccgct
      541 ccgatatcgc cggcgtgaat ttccgtgacg ggagttacgg tcttgccaaa caaaatgccg
      601 atgtgggaaa agcgctccat cgcgccgcgc gtgtagttgt aaacgttcgc gtcgttttta
      661 agcacgccgg atatgacctt gaaataggtg acgcggccgg cgaaaggatc ggcgaccgtc
      721 ttgaaaacga acagtgacag cggctcggca tcggagattt tgcgggaaac cggagccccg
      781 tcggggctcc tgccctgcat ctcgccgcga tcggccggcg caggcatatt ttcggccaga
      841 aaattcagga tgagatccga tcccatattg cggatcgcgg aggcgcataa aatcggcacg
      901 atgcggcgct ggcgaacggc gtcgcgcagg ccctccacga tgtgctccgg cgagagggtg
      961 cccttgtcaa aaaactcctc catcagggcg tcattgccct cggcgatcat ctcgatcaga
     1021 gcctcatgcg ccttttgcgc ggcctccgac aagtcggcgg gaatcgcttc ttcctttgcc
     1081 ttgccgtcgc cgtcggtcgt gtacgcgtag gccttcagcg acaccagatc caccacgccc
     1141 ttgaaatccc gctccgcccc tatgggcaac tggacgggga cggcagtccg cccgaaaatc
     1201 tcatgaatgt tttcgagcga tcgctcgaaa ctcgcccgct cccgatccag cttgtttacg
     1261 acgatggcgc ggggaagctg gtactcgtcg gcgtaggccc agactttctc ggtctgcacc
     1321 tccacgccgc tgacggcatc gaccactacc agggccgagt cggcggcgat cagcgcgctt
     1381 ttcgcgtcgt ggatgaagat gttgtatccg ggggtatcga tgaagttcag cttggtttta
     1441 ttccactcga gaaacgcgac cgccgaggaa atcgtgatct tgcgagcctg ctcttcctcg
     1501 tcaaaatctg tgaccgtatt gccttcctcg acccgagtta gacggttggt ggccccgccc
     1561 gtgtagagga acccggcgac caggctcgtt ttgccgcagt cgccgtggcc gaccactccg
     1621 atattgcgaa tatccttgcc ttcgtagact ttcatacagg aatgattgcc gatcaggggc
     1681 gctaagcccc gcagatagcg gcccttagaa aatcagcgta acgcaaaccg agcatagtat
     1741 cacaagtgga cgaagaacgc cgcccgcccc tggagtcctg gcggcgcaca cggctgccgc
     1801 gtcccaaagc ggcgccggat ccggcggcgt ccggaggctt gctataattt tcgccaggga
     1861 gcagttggta tgtcggtcgt cgaagttgca ttgccgggtc ccgagaccat cgcgcgctac
     1921 gagccggtga tcggcctgga agtgcacgtc cagctgatga ccaggaccaa gctgttttgc
     1981 ggctgctcca cggaattcgg ttcgccgccc aatacccagg tctgtccggt ttgcctggga
     2041 ctgcccggcg ctctgccggt aatcaaccgc gaggcggtcg ccctggccgt ccgcgcggcc
     2101 ctggctctga attgcgaggt tcgctcatcc tcgcgcttcg cccgcaagaa ttacttttat
     2161 ccagacctgc cgaagggata ccagatttcg cagtacgaca agcctctggc cgagcacggc
     2221 tggctggatg tgccgctcga cggcggcttt aagcgggtcg gcgtcacccg cgtgcatctc
     2281 gaggatgacg ccggcaaatc gattcacgac ggctacaagg attcggatcg gtacagctac
     2341 gtcgatctga accgcagcgg cgtgcctctg atcgagatcg taagcgagcc ggacattcgc
     2401 agctccgcgg aagcttacgc ctacctcacc gaattgaagc agatcatgca gtacatttcc
     2461 gtatccacct gcgatatgga aaaggggcat ttgcggtgcg acgccaatgt ctcagtgcgc
     2521 ctgcgcgggg cggccaagtt cggcaccaag gcggaagtga aaaacctgaa ctctttccgc
     2581 tatctccaga aagcgttgga ttacgagatc tcgcggcaag tgggagtgct ggagagcggc
     2641 ggcgccattc tccaggagac gcggctgttc aacgtggaga ccggcgaaac cgtgggaatg
     2701 cgctcgaagg agcacgctca cgactaccgc tacttcccgg agcccgatct gctgccgctc
     2761 gtggtgagtg aaagctggcg cgaacagatc cgcgcttcca tgcccgagct gccggcagcc
     2821 aagcgcaaga gatttgtcga gcaatacggg attcccgatt acgacgcgca ggtcctcacg
     2881 ctggcccccg ctctggcgga ctacttcgag gaagtggcgc ggcagagcgg ggaaccgaaa
     2941 gcggcttcca actgggtgat gggcgagctg accggagcgc tgaaggcagc cggcaagccg
     3001 atcgaggaat cggccgtcag cgccgccaat ctggcggagc tggtgcgcct catcgccgcc
     3061 ggagagatct ccggcaagtt ggctaaggat atttttgcca aaatgttttc gaccggccgc
     3121 tccgcctccg agatcatcga agcggaaggc ctgaagcaga tcagcgacac gggcgagctg
     3181 gaaaagatgg tggacgacat tctcgccgct aaccccaagc aagtcgaaca gtacaagaac
     3241 ggcaagaaag gcgtgctcgg cttctttgta ggacaggtca tgaaggcgac tcgcggccag
     3301 gcgaatccgg cagtagtcaa tgaaatcctc ggccggaagc tgggataggg cgagtcccct
     3361 cgctttgagc gcgtattccc ttcggctcgg taaaatcctc ccccgcgggt tctatgagcg
     3421 tcccgtcgag caggtggcgc gagatttatt gggaaatttt ctgcttcaca agataggaaa
     3481 cacgacgctc gcaggcaaaa ttgtcgagac ggaagcgtac ttgggcgtga acgaccgcgc
     3541 cgcgcacgcc tggcggggcg tcacgcaccg cacgcgcgtt cttttcggcc ctccggggca
     3601 tgcctatgtc tacttcatat atggcatgta tgaatgcctg aatctggtcg cggagcccga
     3661 aggacgcgcc ggctgcgtgt tggttcgcgc cgtcgagccg gtgctgggca tcggcgagat
     3721 gcgccggcgc cgcccgggag ctcggcggct ggaagacctg gcctcgggcc ccggcaagct
     3781 taccctggct atggggatta cgcgtaagca taacggcgcg gacgtgacgc gcgggccgct
     3841 ggtagtgcgc gagccggcga tccgcgaagc ttttcgcatt gccgccggcc cccgcgtcgg
     3901 cattacgcac aacgccgact ggcccttgcg cttctatatc gaaggcagcc cctacgtcag
     3961 ccgcccgggc cgccttcccg cggctgagcc tgctaacgaa cagacggcgt cccacggcgc
     4021 aaaccggaaa aggatttgcc cgggcgaaac ggtataatcg caccatggcc gcgcaggcga
     4081 agcagccttt gacgctggat gttctcgatc tgcggcactt ccaatccccc gattttgacg
     4141 cgctgctggg cgaagaggcc cagatctggg ccgataagct tcactgggat ttccgcccat
     4201 cgtgcgatat cgtccgccgc tatctggacc tgcacgcgtt gagcggctat gcgctgatcg
     4261 agggccgcca gcctgttggg tatagctact atgtacatga agaccacaag gtcctgatcg
     4321 gcgatctctt catttccgaa ccgtaccgca ccctcgaggc ccagcaccaa ttgctgcggc
     4381 acgtggtgga aacggcgcag aacactccgg gcgtccggcg catcgagggg cagctcatga
     4441 tgctcgagtc cgccagcgtc ggctcgctct atccggccgc gcagctctcg atttttgaga
     4501 gaaatttcat gctggcgcag gggatctcga ccctgcggcg cgaggttttc ggcgccgttc
     4561 cgccgggctc gatcgccttt gagcgatggt cggagcagca gatggagcag gcggcgaacc
     4621 tcatcgcccg cgcctatcgc acgcacgtgg atagcgagat caacgatcaa tatcgcagcg
     4681 tttcgggagc ccgccgtttc ctccacaata ttgtgcagta tcccggctgc ggggccttct
     4741 atgcccccgc cgccgtcatg gccctcgacc gcaaaacacg cgagctgtgc ggcatttcgc
     4801 tgaccagcct ggtcaggccg gacgttggtc acatcacgca gatctgcgtt gcgcccgagt
     4861 ggcgcgggtt ggggatcggc tccgagctgc tctggcggtc cattcaggcc ttccgcgaag
     4921 cgggctgcgc cgaggtcagc ctcacggtga ccgccgccaa cacaggggcc gtgcggctat
     4981 acgagcgcgt ggggtttcgt acggtgcgga agttcagggc ttacgtatgg gagaatttcc
     5041 gcaagtagct gtgcgcctga cggtcgtccc cggccgttgt tttcgccgcc ccgccacaaa
     5101 acaaaccggc tgagaccggg cggccccgcg caagaactct cccgttgcaa gagctgtacg
     5161 ctggcgacgg aagcccggtg cggaaatgcg cggcggccaa atacagattg cccttcgcgt
     5221 ctgcccccgc cagcagcccg gccgccggcg ggagggcatc gccgtcaggg gatttagctg
     5281 cggattgaag atttccaaat ccgagtggct gattttttct ccagaggctg ccctggcccc
     5341 ctgccgatat ggcgcggctg cgcgaaaagg gaaacacaga acgccaagcc ggcaagaact
     5401 gttcccattc cgcgcattga ctcttcctct cgtcattacg caccgacgaa ggaaccggca
     5461 ggattggcgc cgctggctca catcttcatc gccaaatcga tcagggtgcg caccgcgatc
     5521 cctgaaggcc ccttggccat atacggcttg gcttcctcga gccaggccgt gccggcgata
     5581 tccaggtgaa cccaaggcgt tgggtcggcg aactctttca gaaacatcgc cgcggtaatc
     5641 gcgccgcccc agcgcgttcc cacattgggc aagtcggcaa tgatgctttt gagctgctcc
     5701 ttgtagtcat catcaagggg catgggccac attttttcgc cgcactccct ggccgaggcg
     5761 agaagccgct caagccaggt ctggtcgttg gtgaaagcgc cgacgttgta atggccgaga
     5821 gccaccacaa tcgctccggt gagcgtggcg gcatccacca gatgggtgca gccctgctgg
     5881 cgggcatagg tcatggcgtc ggccaagatt aagcgcccct cggcgtcggt gttgatgact
     5941 tcgactgttt tacccgacat ggtggtctga acgtcccctg gccgctgggc gcggctgcca
     6001 ggcatgtttt ccacgctggg aatgaacgcc gagaccggaa ccgacggctt caggtcagcc
     6061 agcgcccgca tcacagcgat cattgcggcg ccgccagcca tatcgtattt catcttttcc
     6121 atgccctcgg caggcttgat cgagatgccg cccgtgtcga aggtgacggc tttgcccacc
     6181 agccccagat ggacgtcgga agaaggcgga gaagagggcc gataccgtag caaaatcagg
     6241 gccggcggtt cggcgctgcc ctgcgccacg ccgagcaaag cgcccatgcc cagcgcccgc
     6301 atccgctgct cgtccaggat ctcgatttcc agaccctttt cagccgccat ctgctgagcg
     6361 cgggcggcca gatctttcgg agccaggcgg ttggcgggct cgttgactag gccgcgggca
     6421 aaattctggc actcaccggc gataacggcg cggcgcaagg cagcttccag cttcgggccg
     6481 ttgttgtcag aaaccgcgac ggaagcctga tcgatccgct tgtcgtcttt cttatccgtt
     6541 ttgtaggaat ccggctcgaa atcggcagcc agcatgccct cggcggccgc cgccacctga
     6601 gcttccggcg aaaagccgcc cgaaggcgcg aaaacaaact cgtggacgcc tctacccttc
     6661 agatagcgga gggctgtgcc ggcggccttg cgtaactgcg ccgcgtcgaa tttctccact
     6721 ttgcctccgc ccaccagaag cagacgcttc gccgcgagac ccgtcgggtg gtggaggagc
     6781 acggtgtcga gagcttttcc ggtggtttcg ccggatgcgt acagttccga gagaaggccg
     6841 ccggtctgcg cgtcgagacg gcggaacggc tcatcggcgg gggccccgtt ggcgctctcg
     6901 aaaaccacaa ccgccaacgc ttcagtagct atagtttcct tcggctggga gttcagcgtg
     6961 agtttcatgt ttacaggctg cggcggcgca tggcttcgcg ggcctcggcc tcggctgtcc
     7021 gggcgcgttc cgcctcgcgt ttatcgtgca agtgcttgcc tttggccagc gccagctcgc
     7081 atttgatcct tcctttcttg agatagaaac gaagcgggat gagggccagc cctttctccc
     7141 gcgttttgcc aatcagtttt tgaatctctt gcctgtgcag cagcagcttg cgggctcgca
     7201 ccggatcgtg gttctcgcgg ttgccgtgcg aatagtggct gatgtgggcg ttccgcagcc
     7261 aggcttctcc ggcttcgatc gagacgtagc tgtctttcag ttgtactttg ccttcacgcg
     7321 cggctttgac ttcggtcccg gtgagcgcca gaccagcctc aaaccgttcc agcagaaagt
     7381 aatcatgggc ggcccgccgg ttggcgctca gcacccgttc ttcgttagtt gcggggttag
     7441 ccatgggccg gaactgtaaa attcactcca cgcggggcgt gcggcgcccg tcaagtattg
     7501 atggatcgcg cggagtgtcc cagcgctatc tggtgaaatg actgatttta acataaagcg
     7561 tgccagccgt ttcgcagccc tgtgtctctc gttatggctg gccggagggg gcttggttcc
     7621 gctggcggcg gcgcccaagg gcaatgccat tttcaagcag ggccgtaagg ccgagctcag
     7681 caaagactac gacaaggcgc tcgaactcta tgagcgcgcg ctgagagaag acccggaaaa
     7741 ccagacgtac atgctcgcca cccgccgcat gcgctttgtt gcgggccaga ctcatgtaga
     7801 ccagggccac cggctgctcg atcagggcaa gctggtggaa gcggcggctg aattcgagaa
     7861 ggcaaacacg atcgatccct cgctgactat cgcccagcag gaactcaagc ggacactcga
     7921 tctgatcgcc aaaagagagc aagagcaaaa acaaaaagag ggacaaaacc agccgggcgg
     7981 cggtgcgggg gcggcccagc ctgccaagcc cggcggcgcg ggagcggccg aggcccagaa
     8041 gcccgagccg ggcctctccc cgctgctcga agcgaagaaa gaaaccgagg aactgctcga
     8101 gtcggcgcag cctgtgccga cgctccggcc gctctccacc caacctatca acttcaaagc
     8161 cagcaacaac agcaaggtga ttttcgaaac catcgggaag ctggcgggca tcaatgtgct
     8221 attcgatccg gagatgcagg ggcgccggat taccgtcgag atcaacaacg ccacgcttct
     8281 gcaagcgctg gattacgtgg cgacgatttc caaggcctat tggaagccgc tttcggccaa
     8341 cgcgattttc gtcaccgacg acaataccac caagcgccgc gactacgaag attacgtggt
     8401 ccggacgttc tatttaagca acgtgtatac ggcccaggag ctccaagaaa ttgcgaccgc
     8461 cgtgcggtcg gtcactgaaa tccgccgtgt tttcaccatc aactcgctga acgcgctggt
     8521 gattcgcggc accgccgatc aactggcgct ggccgaaaag gtcatcgacg atgtagataa
     8581 gtcgcggggc gaggtgatca tcgacgtgtt agtgctcgag accagccgga cgacggcgcg
     8641 cgacctcgga ctcacgccag tttccggcac gacgccggga atcaatgtct cggtcgctcc
     8701 tgtctcgggc agcgttacaa ccgggacgag taccggcacg accggcacaa caaccaccgg
     8761 cacgactacg accgggacga ccactcccac tacggggacg gcaggaacca ccacgactcc
     8821 cgccgccggc acttatacct tcggccattt gcaaggaaac agcttttcgc tgagtttgcc
     8881 cagcgccaat ttgaaagcac tgatgagcgc ttccgatacg cgcattttac agtcgccgcg
     8941 agtacgcggg tttgacggct acaagaccag cctgcgtatc ggtcagcgca tcccgattgc
     9001 caccggcagc ttccagccgg gcatcggcgg cgtggggatc aaccctctgg tgaataccca
     9061 gttcaattat caggatgttg gcgtgaacgt ggacctgacc cccagggtgc actacggccg
     9121 ggaagtctcc atgcacatcg aaattgaaat ctcgacggtc gccgaccacg tcgatatcgg
     9181 cggcatttcc cagccggtta tcggccagcg caagatcatc catgatttgc gcctgaaaga
     9241 gggagaaagc agcatcatcg gcggcctgaa ccagacccag gtgacgagga ccgtggccgg
     9301 cgtcccgggc ctgatgcaga ttcccgtgct ggggcggttg ttttccaacg agcacaagga
     9361 ggtcagcgac caggagattt tgattgtgct cacgccgcac gtggtgcggg tgccggagct
     9421 gaccgacctg aacatgaagg gcatcagcgc cggcaccgac cagatcgtga gattgaattt
     9481 tgctccgcct ccgaatgatg gtccggcgct gcctcctctc ccgccacccg gaggaactgc
     9541 tccggcagga gctccggctc cggcaacgcc cggcgcggcg ccggcggcgc cggcagttcc
     9601 cacggccccg gccgctccgg cgggagcccc ggcgcagcca accgttccgc cgcccgtgcc
     9661 gggcgctgct attgctccca caccgccgcc gggaccacgc ggccccgggc ccagaccggt
     9721 gactccgggc gccgcccagc cggcggcgcc gcgcggagcg gaagctgctg ccccgcggct
     9781 gcggtttgaa tcggcgcagg tgagcggagc tatgggaacc cgtatcacgc tgaacctgct
     9841 ggcggaaaac gtgaccgatc ttttcgccgc gccagtgcgg gtcacattca atccgaagca
     9901 gttgagcctg gtggacgtgc gccggggagg gcttctggcg agtgacggtc aagacatcat
     9961 cttctcccgg aacattcaaa atgacagcgg ggaggcttcg atcaatctgt cacgattccc
    10021 cgggtccggc ggggtttcgg gcagcgggtc gttgcttacg ctcgagttcc agcccgtggc
    10081 tgccggtagc tcgaccgtaa gcgtcaccgg cgccggcgtc cggaacgccc ggcaggaagc
    10141 gatcaccctg ggcggagctc aagcagaggt cgtggtccga taatagagta caggggcccg
    10201 gtcgtgagcg gtagattggc agcgagagcc ggcgcggaga gacgcaccgt tggccgcaca
    10261 ggtccgccgg ggaaaaaggc agcgcggccg gaatctggcc tgacactgat cgagctgatc
    10321 gtggcattca cgatcatgct gattctgacg acgatggccg tgccgctggc gcgcgtgaag
    10381 gtgcgccgcg aaaaagagcg ggaactgcgc tacgcccttc gtgaaatccg ctcggcgatt
    10441 gaccgttaca aagatgcgtc ggaccgcggt ctgctcggcc ctcctaagct cgaatcggaa
    10501 gggtatccgc ccgacttgca gaccatggtg gacggagtaa agatgcaggg gacggtagac
    10561 aagaaaatcc ggtttctccg gcgcatccct attgatccaa tgacaggaac ggccgactgg
    10621 gggcttcgct ccatgcagga cgacccgacc tcgcaaagct ggggcgggca aaacgtgttc
    10681 gatgtttaca gcaaaagcat ggatacggcg ctggatggaa ccaaatattc ggattggtaa
    10741 cgaaggcagc cgtgcgcgtt ttctacccgg catggccaag ggcagtcccg gagcgccaat
    10801 ggctggaagc cggctgggga aaggcggcgc gaaccgaggc tttacgccat gaagaaccca
    10861 cgttccaggg cagggatgac cctgatcgag ctgatgatcg tacttacgat catcatcacg
    10921 gtcatttcgg tggccgtccc cctctatcag aaggctatca tccgttccaa agagagcgtg
    10981 ctgaagcaga atcttttcac catgcgcacg ctcatcgacg agtacacgtt cgacaaggag
    11041 aaagcgccac aatcgctgga ggatctcgtg accgagggct accttcggga actgcccatg
    11101 gatccgttca ccaagaagcg cgacacctgg aaggtggtca tggaggacac gctgcaatcg
    11161 gtggatcaaa aggaaccggg catcttcgac gtgcacagcg ggtatgacgg aactgcgctg
    11221 gatggaacca agtacagcga ttggtgagag aggctcctct attatggccc gttgcgcccg
    11281 gcggctttcg gcgtgcgtca gtgtttgaac cggtacccca aaatcatttt gattagcaga
    11341 tgatcagtcg ccgcggtcgt ccccaccccc accccgaagt tgaactccca gttgggcgaa
    11401 agcacgaggt caacggcggg caaaatctgc tgctgctgct cacgcagcgg gtcaaaaccc
    11461 gtcaccgggc ccaaggcgcc gtaatactcg aaaccggctt ggactttctt ggcttgttcc
    11521 ccttcccctt tcacccatac gtcatagctg agcttgaagt ttggggaaaa ctcaaagcct
    11581 ttgctgacgc tcgggccatg aaaagcgcgg tcaaaggtcg gattaaagga aaaataccag
    11641 cgattgattt ctttgtcgat gatggggcga atttcccacg tccaggtatc aggcgagtag
    11701 acggcgcgct ggtatccgaa ctcttgcgaa aggctgaggc ctaccggcaa atgccagctt
    11761 tccggaacgc ggaaacgcgg ccggatatgg tcgcccaccc aggaccagcc gtagcctgat
    11821 tgaacgctgg tgaagatgta gaagccggtc tcaaaccacg gggtgaagcc atgggtgatc
    11881 tcgatggtct catggtattg gtgattggta gggcgggtgc cgtcgtcggt ggtcttgctg
    11941 ccctcgagag taaaattcat gtggctctcg accattgtgc gcccgggctc cacggtttca
    12001 tacggatata cttgcacctc atagtttccc tggccgtaaa gggcggcggg cccaaggcag
    12061 agaaacgcga gcgcccattt cctacgccac aacgctgttc cagcatgcgc gcataatcgc
    12121 atcgatatac ccaaaggccc tgtccagact caaaaggtta ctccgtcttc tggggttctg
    12181 acaaccttcg ggagcgtaat gttatgctga taacccgagg gattgtggca tgaagaaatt
    12241 tcccggcgtg tgcctgctcg cggcagccct ggcctgggga tctcccggcc cctcggccaa
    12301 cattttcccc ttccactact ccatcgacga tctgccgaac gggctacggt tgatcaccgt
    12361 gcccacgggg cttccgaata ttgtggcggt ctatacggtt gtgcgcaccg gatcccgcaa
    12421 cgaggtggaa ccgggaaaat ccgggtttgc tcatcttttc gaacacctgg ctttccgcgg
    12481 gacagagaag tatccggccg acaaatggga ggctgtgatg caggccgcgg gcgcgcaaac
    12541 caacgcgtat accaccgacg accgcacggt ctatcacgag gtattcgcga aggaagggct
    12601 ggaatcggtg ctcgcggccg agggcgaccg ctttcaaaac ctgaaatact ccgaacagga
    12661 cttcaaaacc gaaacccggg cagtgctggg agagtacaac aaaaacagcg ccagtccgtt
    12721 cgagaagctc gacgaggtgg tgcaggcgac cgccttcacc cggcacacgt accggcatac
    12781 gacgatgggt tttgttcagg atgtggaaaa catgccgaac gagtattcct acggtctgga
    12841 attcttccgc cgctggtacc gccccgagaa cacggtgatc tgcgtcgtcg gcgacgtaga
    12901 ccgccgcaca accttggcgc tggtcaagaa atatttcggg ggctggaaac ggggcagcta
    12961 caaggcggag atccccgcgg agccgccgca gaccgagcct cgctcggctc acgtcgattg
    13021 gcctgcgccc acactgcctt tggtgacggt ggcgttcaag gctcccgcct atgacgacga
    13081 aaccaaagat tcggcggccc tcgacattat cctgtttcag gccttctcgc aaaattcgga
    13141 tctttacaga aagctggtga tcaacgagca aaaggtcgat ctgctctcgg ccgacaatcc
    13201 cgaccacatc gatccctttc tgtttgaggt gaccgcccgg gtgaaggatc ccaaagacgt
    13261 cgactacgtc cagcagcaga ttcttcagac ctgccgggac ctccaggaaa agccgcttcc
    13321 ccgccagagg ctggacgaag tcaagtcgca cctcatttat agcttcgccc tcggcatgaa
    13381 cagcacggcg cgcgtggcag aaacgctagc tgcttatgtg gggctgcggc ggacgccgga
    13441 aacgattaac aaacgctatg cgctgtaccg gtctattacg ccggaagatg tacaggccgc
    13501 ggcgaaaaag tactttgttc cgcagggccg cacgattgtg acgctcagcc ataaggcgga
    13561 aggaagaaca tccggaacag gagaggcccg atgaaccggc agcggagatt tcgcgcctcc
    13621 ttaggccgct tcggcctgtt tgtgctggct gcaatcgcct tgtcgcatgc cgcttgggcg
    13681 gcgcagcaga tagaaactgt cgcgctcccc tcggattcgc cgctggtcag cttccggatt
    13741 ttgtttaaaa ccggcgcagc ttccgatccc gcgggcaagg agggcattgc cgctctgacc
    13801 gcggcgatgc tcgcgcaggg cggttcgcgc gacagaagct acgagcagat tctcagcgcc
    13861 ctgtttccga tggcgtcggc tgtccggtcg caagtcgata aggagatgac cgtcttttcc
    13921 ggcactacgc acatagacaa tctggagaag tactacgcgc tgatcaggtc tatgctgctc
    13981 gatccgggct ggaggccgga cgacctccgg cgcttgcgca cggacacgat caaccggctt
    14041 cgcgtggagc tgcgcagcaa caatgaagag gaactgggca aagaggcgct gtatcttcgc
    14101 atctatggcc ccgcgcatcc gtacgggcac gaaaatctcg gcgccgtgtc atcgctggag
    14161 aaattgagcc tggacgacct ccaggccttt taccggcagc actacacgcg ggccaatctc
    14221 gtcattgggc tggcgggcgg ctttccgaag gattttctcg agaaggtacg ggccgatttt
    14281 tcccgcctgc cagagggggc gcaatccacg ccatcggatt tgccgcagcc tgagcccgcg
    14341 agcaagctgc gggttttgct catcgagaaa aatacccgcg cgacagccat ctcgctcggg
    14401 tttccgctgc gcgtgaaccg ctcttcgaaa gactggcccg cgctgtttct ggcgcagacg
    14461 tactttggag cgcatcgctc gagcaacggc gtgctgtttc acgacctgcg cgaggtgcgg
    14521 gggctcaatt acggggatta tgcctatatc gaatacttcc ctcgcggaat gttccagttc
    14581 gagcccgacc ccaacctggc ccggcgccag cagattttcc aaatctggat tcgtcccgtc
    14641 gagccgcaga acggtatgtt cgccctacgt gcggcccttt atgagctgga taaactggtg
    14701 aaggagggcc tttccaaaga gaaattcgaa gcgacgcgca gctatctctc gaaattcgtc
    14761 aacttgttga cgcagacccc cagccagcag cttggctacg ccatcgacag caaatattac
    14821 ggccttcccg atttcaacgg ctacgtcaaa aacgcgctgg cccgcctgac gctcgaggag
    14881 gtcaacgcgg cgatcaggag aaatcttcgc tcgagcgatc tggatatagt cattatcacg
    14941 aaggacgcgc aggggatgaa aaaggccatt ctctccggag aaccttcggc gatctcttat
    15001 gtttcgccaa agccccccga ggtggcggcg gaagataaga tcatcggttc gtacaagctc
    15061 gatgtgagca gcgtggatat cgtccccgcc gagagccttt ttgaaaacta gcccggaccg
    15121 cgtctccggg catgggttcc gaggccgcct tcaacttgac gccgcgcttt gcaaatcagg
    15181 tagaatttaa aacttcgcgc tttccggcga gaatgcagga atgcgcattc gcgcgtgcaa
    15241 taggccgagt cgccgaacac gatcagacgg aattcgccga tcgactggat caagaagaac
    15301 gggcttcaat aacccggagc gggctgcaag cccgcagtca gggcccaggg cgaacgtatg
    15361 ctgcaaccag gagtcacgca ccaagaaacg atggtggtca ccgaggccga ggcgatccat
    15421 ttcatgggca gcggcatccg ggcgctgtca acgccctcga tgattctgtg gctcgagatt
    15481 accgcgcgaa acgccgtgct gccgctgctc gagccgggac aggatacagt gggcacgctg
    15541 gtgaatgtgg cgcatctggc ggcaacgccg ctggggatga aagtaacttg ccgcgcgacg
    15601 ctcaacgcca tcgaccgccg ccgcctgacg ttcgccgtgg aagcatatga cgaaaaggag
    15661 aaaatcggcg aaggcacgca cgagcgtttc gtgatcgatg tacagcgata tgcggaacgg
    15721 ctcagcgcaa aaatgcgcgc ctgagcattt tttctacttg gataaaaaag ggaaagtcag
    15781 atccggtggc gcggcgattc attccattgt ccatttttgg cgcccgcagc tcatcagaga
    15841 aaaagctctc tcctacctgg cggcgttcca tgccatttta tctactggcg caccgtccac
    15901 ttgatgacca cggcggaccg cagccgcttc aagctgcgca gatagaggcg cttgtaatga
    15961 ttggcctcgc tagggccctc tacgatggca agcgagtccg agggaacgtc aatggtcacc
    16021 ggcacgcccg gcagttcgcc ggtcagccgg cccggcgaaa cgcgatcacc ctcgatacga
    16081 atcaacgtgt ccttttccac ctggccggac caaatcagag tacccgcgga cggcccggga
    16141 taggggaccg gttcgggggc ggcggcaggc tcgcctggag ccgccggggc tctgtgtagg
    16201 gtcaggcgcg aggcagcggc atcggagccg tacgagggcc cgggctgcga agccgatggt
    16261 gcggccgccg gcgctggcgg cgtttctgag ggatcgggct tgagcaggtg aattcgcggc
    16321 cgagatggac gggacggcgg gggcgaagct tcactggccg gaggggcggc agtgggcgct
    16381 ccggcggccg gttccgatga cgtggcggcg gagttagcca ccgaatcggg cgccggggat
    16441 cgagccgctt gcgccgattg tacggctgcc tgattttttg gcaggcggct cgctgcggcg
    16501 tccccaacat ccggggccag cttcgcttct ttcccgctgg cctccttccg gtgggtcgaa
    16561 gcgggctcgc catcgcggcg agaggccccg gattcaccac cgcgcgccgg cacggacggc
    16621 ggcgattctt cgaggcggtt cgcgctttcg ccagccgggg ggcgctgctc tccggctggc
    16681 gcggtggcaa ccgtcagctc gaccgagcca gggttcgccc ggcgaagcga taacagccgg
    16741 tataaaagcg cagctccggt tgcaaccaga acgagcaaag cggcgatcca gagccaacgg
    16801 gaaccgctgg agccgcgcgg cgcatccgtg gcgccgctgg gggctgcggc cgcacgggat
    16861 gccaccggcg ccccgcaatg ctggcaaaac ttctcgcccg cgctgatttg cattccacag
    16921 tttgcgcact tcataaaaac attatcgaac gcttccacgc gccccggatt tcccccgggc
    16981 gctctagata aacgccggag agccatcggc ccggtattga aaggcgcgcc gccagggctt
    17041 cggcggatag tgtggaaacg cctcttcgtt aagctccact ccaaagcccg gtttggtggg
    17101 cagtggaata tatccgttct tgaccatcac cggctcctga agcagatcct tgcggggggc
    17161 ttggtcgtcg ggatggtatt caagaatgag aaaattggga gtggatgcgg caaaatgcac
    17221 attcaccacc gtggccagcg gcccgacggg gttgtgcggc gccaccatta cgctgtgggc
    17281 ttcggccatg gcagcgatct tcttcatctc cagcagcccg ccgcacacgg tgacatccgg
    17341 ttgaataata tcaacgcact gtgccgccag gatcgggcgg aattcgtgct tggtgtaatt
    17401 ttcttcgccg gtagcaagcg ggatgttgac gtgcgccgca agtttcgagt aggcggccac
    17461 gttttcaggg cgcagcggct cttccagcca gaagggctga tagggttcca accgctgggc
    17521 catacggatc gcccgaatcg gctcaaagaa aaccgcgtgc gcgtccacgg cgatatccac
    17581 gtcggggccg accgcgtcgc gtacggcaga gatgcgctgg cccgcggcgc gcgtcaccgc
    17641 gttgtacggc atcgcgttgc ctccgggcgg atgcgggccg atcttcacgg ccgtgtaacc
    17701 gtacttctgc accagcgcct tggcgttggc gccaagctcc ctggcgtcgc ggccgccggg
    17761 gctttgatac acgcgcacct tgtcccggat tttgccgccg agcagcatat aaacgggcag
    17821 gccggcggct ttgccggaga tgtcccagag agcgtgctcg atgccgctga tcgccgcgtt
    17881 gaccaccgag ccgccgggga agcgcgtgaa gttgtacatg gtggcccaaa ggtactcgac
    17941 gttccggggg tccatgccga cgatccagct cttgaaatcg ttgatggccg ctacagtagc
    18001 ctcgtcgggt cccaccgagt aagcctcacc aataccatga atgccctgat ccgtgaggac
    18061 cttgacgaaa atgtaattgc gcccgcccac tccgaccatg taggtcttga tgtcggtgat
    18121 tttgatggct ggacggccgc cggcgggatc gctgctcagc cctgctctct ggctgccagc
    18181 ctgtggcatg gcggcagcca gtaaaggaac gccagacccg gccaggaatt cacgtcgttt
    18241 catgttcgcc tccggcgttc agtgtatacc cgcgccgggg cgggcagaag tcagcctttc
    18301 cggattttta ccggggcttg caggcggaag gtaagcaggg cttcggccgg cagcaggaca
    18361 tccttcttgc ccgtggcata agctccggca gtgccggccc ccacgcccgc ggcggcgcca
    18421 atcagcgctc cggtgccgcc gccggccagg ccgccaatca aagcgccagc tccggaaccg
    18481 ccgccgatca agaccaaatt ccgctttttg tgcgatttgg ccgaacggct tacgcgggta
    18541 gttttgatgg aatattcctg gtcctgccag gcgagagtat caagcgtaat ggacaagcgg
    18601 gcgggcgtcc gcaggcggcc ggaagcgacg gcgctgacga catgtccacg cactacggcc
    18661 cccttgggta cgacgacgcg gtcgccgagc actacgggct ggttcagcgt agcttcaaac
    18721 ttttctcccg ctgcgctgcc cgccgaaccg agcggctggg cgagccgcac ttgcagctcc
    18781 gtaccggcag gaatctcgac ctccgcagcg gcggggggct gcacgggttc ggccgggaca
    18841 gccgcggcag tgcttgcgcc tgtcgcagcg gcgggcggcg tcaaatcgga tgagccagcg
    18901 gtgttcatgc cggcaggctt cttcgcgcaa gccgacgtcc agagaagacc ggcgaggcag
    18961 cacgcggata accagtacgt atgacttttc acggagcacc tcccgaacca gctcagcaag
    19021 tccgcagcca ggcccgaaaa actctttaaa actagctact taaagttgca tccaaggtct
    19081 cgcggcggcg cctgccgtgt aaattttgca gtggttacgc ttgctgggag ccggccgctc
    19141 attctttgac tcggacggaa tcggtctccg ccaccctcaa cggccgtctt cgcgagtgga
    19201 agactggcgc cgcgggatgc tggtcgacgc gggcgccgac gcagcggcgg aatctgacgc
    19261 ctgcgttcgc ggcgatgcgg ggtgtggctg acaagatcgg ctacttttcc acagcctgga
    19321 agtcgcaagg ctcagcccag agccggttcc atgggcatcg ccagaggagg ctccagcaga
    19381 aaatcgagat ggctcatttt tgccaccgct tcgtttaccg cgcgctcgga agtcggctca
    19441 agcgtgatca caaacggcag atcctgcttg ttttcgcaag gctcttgcag cacggcttcc
    19501 aggctgatat gggccgaagc caggatcgcg gcaagagagg cgagaatacc gggacggtcg
    19561 attacgcgaa accttaggta atacggccgc acttggtgtg tgatcggaat tggagcgtac
    19621 tcgcgcaagc ggtcgtgggc aaagggcgaa acgcgctcgg gactcccatt gacaatctcg
    19681 cgggcgacgc gcatcagatc gctcaccacc gccactccgg tcggcagcga tcccgcgcct
    19741 ttgccgtagt aaaacgtatc agcgccatgc gcgcccttga cccaaatcgc attgtaggcg
    19801 ccgtccacgt gagccataat tgccgagagc gggatcagcg cggggcgcac ggaaagcaag
    19861 aggccctcgg gggtctgccg cgctgcgccg atcaggcgaa tggtgtgccc aaggcggtgc
    19921 gcgtaagaaa aatccatcgg cgaaatacgg cggattccct ggacataaat atcggcaggc
    19981 gacacgcgca cgccgtaagc cagggcggcc agaatggcga gcttcgagcg ggcgtcgtaa
    20041 ccatcgatgt cggcgctggg gtcggcttcg gcataaccca gccgttgcgc ctcggcgaga
    20101 atcgcgggca gcggttcgcg ccgcttctcc atctcggtca atatgaaatt gcaagtgccg
    20161 ttgagaatgc cgaaaagagc ttcaacgcgg tctccagaga ttccctcgcg cagaacggcg
    20221 tgaatcggaa tgcctcccgc cacgctggct tccatcgcca ggcagatgcc ggcgcgaatg
    20281 gcctcgtccc aaatgcgtgg tccctcggcg gccatgagct ctttgttcgc ggtgacgaca
    20341 gacttgccgt ggcggatgga agcgcaaaca atttccgccg ccacgcccga gccgcccacc
    20401 agttccgcta cgatgtgaat ctcaggatga gagacgacgt cgcgccagtc ggccgtatgc
    20461 agggcatcgg gaaagagccg cgcggcggcc ggagggcgat cccggatcga gcgcgagcaa
    20521 agcgctttta cagcgagcgg aaacccaagc ttggcctcaa tttgccgggc attctcgtgc
    20581 aggattgtca gcgtcccgct gccgacgttt cccagcccga caatgcccac gttgatgacc
    20641 ggcataagca gccattctag caagcggtta tgataggagc ggagtggtta tgacgctgcc
    20701 aattgggacg agcgtgctgc tgcttttgtt gttgggggcc ccacagaggc cctatccgcc
    20761 ggagcggaca ggaccggcag gagccgaagg aagaccggac gtcctgctgc caagcggcaa
    20821 gagccagcgg caggagatcc tgaaagccga ctttgcaaaa accaaagcgg atgccgctca
    20881 gctcttggat ctcgcgcagc agttaaaaca ggagctcgat agagacgacc acaacgtact
    20941 tgacctccgg gcaatgaaga acgccgaaga gatcgaaaag ctcgccaagc ggatcaagga
    21001 ccgcatgaag cagcatctct gaaaatagct ccggcctatt gcgcgccgct ggatattttt
    21061 gagaagatcc tcccgaaagg gtgtccccgt tttcacagtc ccgccgatga agacgctcct
    21121 cacaatgcgc gcgagagtgc tcgcgctggc gctgatcacc gcgctccccg gcgcgaatac
    21181 cggctgcaac agcctgaaat ccgttcaggc gcagccggcc aagaacaagg aaaccctaga
    21241 tctcgggaag gtaacgcgca agcggcagat tgcgcccgtt tgcagcgacg cggagtggct
    21301 gacgcggccg gagcgcgacg ccaccgaaca gccggaaaag gttctcgatg cgctggaaat
    21361 accggagggc gccacagtag ccgacgtggg ggccggcgtg ggctacttta cctggcggct
    21421 ggcgaggagg gtgggaccga aagggaaggt gatcgcagaa gatgtccagc agcacatgct
    21481 ggatttactt gcggaaaacc tgaagcagcg ccgaatcacc aacgtggaaa tggtgctggg
    21541 aggggtccgg gatccgcact tgccggagaa tgctgtggat ttggcgctgc tggtagacgt
    21601 ctatcatgaa ttcgccgagc cggaagcgat gatccaccat atccggcagg cgttgaaacc
    21661 cgacggccgg ctggtgctgg tcgaataccg caaggaagat ccgtctattc cgattctgcc
    21721 gctgcataaa atgactgtgg aagaagtacg gtcggaaatc gagccgctcg gcttccagct
    21781 ccaaaaaaca ctggaattcc tgcccacgca gcacatcatc attttcacga agaagccgct
    21841 cccctcggag gagagggctg cggcctcacg ctgaatcggc cggcacggga ggcgcgcact
    21901 ccaaaagttt ccgcgggagc aggtcgcgga ctgttgagac gacgccgtgg agcatgaggt
    21961 agccttctcc gcgctccata tcgcgcgaaa gctggagcca gccgtcgtcg acggaaggac
    22021 cgaaaggcac ccgcatggtc cagcagcggt ctgaacagcg gtcctctccg ccgggatgca
    22081 gccatacatg cagccgcacg ccgccgggaa gtcccaattc gacggcatcg aatcccagct
    22141 cgcgcatggc gtccacggtg agcctccagc actcatcgat cgattcggcg cgctcgaggg
    22201 cataacgata cgagcgcaat ttggtttgag aagcgatgag ctggcgcaca gtgccgctca
    22261 ggatgatctt gcgagcctcg ctgaactcgg cgtagccgag atgctggacg cccatccagg
    22321 aaaccgcgca gaacaaaata atcaccagtc cgccatatcg gaactgcggc atggcggcca
    22381 gcagggaaat catcgcgcac acgctggtga tcgcgtagat tagcaggacg gcacggcggg
    22441 gcgtgaggcc aagatctaca agccggtgat ggatgtggcc gcgatcggcg ctgaaaatgg
    22501 aatcgccgcg caaaaagcgc cgggcgatcg agatgcccgc ttccatgatc ggaaaggcga
    22561 gggccatcaa aggcgccgtc attcccagca gcgtaatcga tttttgcgac cacaaaagcc
    22621 cgtaggcgcc gagaagaaat ccaatcgtca agctcccgct gtcacccaga aacatcgaag
    22681 ccgggttgaa attgaagcgc aggaaggcga gcagggcccc ggcgagcggg gcggtgagca
    22741 gtcccaaaac caccaggccc gaggattgca acatggaggc caccagcatc gtgcaggtgg
    22801 cgaagaagcc gacgcccgag gccagaccgt ccatgccgtc gatcaaattg aatgcattgg
    22861 cgcagccgac aagccaaagc agagtcagcg ggaagctcag ccactgcacg tgcaccgcgg
    22921 tggacccgaa cacatcgtga atttgaatgc catagaaata ca
//
LOCUS       DASREK010000052        17545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_361215, whole genome shotgun sequence.
ACCESSION   DASREK010000052 DASREK010000000
VERSION     DASREK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17545
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361215"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1053)
                     /locus_tag="VFA54_03150"
     CDS             complement(97..1053)
                     /locus_tag="VFA54_03150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZS49831.1"
                     /translation="MRILIAEDEPISRHLLQKFLVQWGYETVVANDGDEAWWTLQRDD
                     SPNLAILDWMMPGMDGLQLCREVRKRTGHPYVYILLLTAKSQKEDVVEGLEAGADDYL
                     TKPFDPDELRVRLRAGRRILDLQEHLLSMREAARFPATHDFLTGVWNREAVLGMLRQK
                     VSRERRETAPVGLILVHLDRFRQVAMNHGPTAAESVLREAARRLRSAVRVYDSIGRYA
                     SAEFLVVVPGCYSSEALEQAEKLRACISDEPVDLLGASISITASVGVASTEDAPNPDA
                     DLLLQAVNVAVRRAKSGGRNRVEFEDLSGLTETRSAAPSEQR"
     gene            complement(1053..4205)
                     /locus_tag="VFA54_03155"
     CDS             complement(1053..4205)
                     /locus_tag="VFA54_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS49832.1"
                     /translation="MEMLLDGKFAPFACIAVSFAPWARNLSNLAIAVSCCVMSASLLY
                     FAGKRRDMPFRGVLHLFAVFLIACGALHLAQIGGEQFQAPLEAVTAGVSLVAALLFLP
                     VIPKALALRSPIELEEARRLLAGQIAERKQAEEKLVLAASFPSQNPNPFVEADLAGNV
                     TYVNPAAQRQFPDLEASGPDHPILENLAPIIQSFRWGVEDSMAREIQVNGAIFQQKIC
                     YVARRDRILIYMVDITGLKRAQEALREGEERYRSLFEEAPIAYHEIDRDGILRRVNQA
                     ECALLGFPASEILGKPAWQFVAREQREESERTVRRKIAGQEPLAPVRREYVRRDGARL
                     TVEIHENFIRGAEGEIAGMRCALLDITDRRRAEEESQRAREAAEAASRAKSEFVANMS
                     HEIRTPLNGIIGMTELALGTDLTAEQRECLQAVKSSADALLAVINDILDFSKIEAGKL
                     ELDPVAFNLRDSIGDALGAVALRGQQKGIELAYRVAADVPETLVGDPWRLRQILINLV
                     GNAIKFTERGEVVVHVGLAEQQSAGKADPIFLHFAVTDTGIGIPKDKQQAIFEAFQQA
                     DTSTTRKYGGTGLGLTISSRLVQLMGGKIWVESEPGKGSTFHFTANLGAAEDPRQLAK
                     PVPASLQGLAVLVVDDNATAGQILAEILAGWRMRPQAAGSADEALAALAAGVEKRDPF
                     SLILIDAQMPEVDGITLAERIRDRPEFGSPKLLLLSPAGQVGDAARRPDLGIAACVSK
                     PVRPSSLLVSLMKALDMAPAEATLAPIGGTAAVSLHILLAEDNPVNQTLARRLLEKRG
                     HTVVVAGNGREAIAALEAEPFDLVLMDVQMPEMGGFEATMAIRAREKLTGRNTPIVAM
                     TAHVMKGDKDRCLESGMNAYISKPIDAAELFSTIEKLVPAKSAGARSQPKGNVMKLPA
                     TPGRPAASSGSSSPDAVKGRNGHSSLNARELLARAQGDWELVRTLAGLFRAECPKFLL
                     KIEKAVAERDTKALETAAHTFKGSLSNLSALAASEAARQLEMAARQGDLEKAQELYAA
                     LEREIARLEPELITLDKELA"
     gene            4556..6076
                     /locus_tag="VFA54_03160"
     CDS             4556..6076
                     /locus_tag="VFA54_03160"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZS49833.1"
                     /translation="MLLRWRLLALAAMFPAAAQQPSTGALDIYITSGGKPAAARVYVW
                     DANGRPLLIPGAVTYSLREESHSVIDQMATAMLPPGKYRVRAEKGMEFQAAEKTAAVT
                     PGEAARIDLEVSRLYNMNGQGWYSGDLHIHRSPEDMPLLLRAEDLNVAPVITRHLGSG
                     TEEGPPFTETPLRSVDQNHIVSFQNQEVERLGKGHGAILLLNTRRAIDSNMAPLFPPD
                     RDFAREARAQGGFVDAEKPIWKNVPVNLAFGLVDSVGVVNNHFHPHGVLLEAEKYGSM
                     EREGAIYKGAAGFAQWMIDLYYSFLNCGFRLAVSAGSASGIMPSWPGYERTYVHLSGP
                     FSYEQWFRDLQAGRSIATNGPLLEVYMNGQPPGAAVDWEAPAGVTLAIDAHAQERLDR
                     VEIVYNGEVIRSFSAGGNTVFRTALNLTITEPGWLAVRCFEPVRETVRYAHTSPFYFL
                     REGKLPVKRPAALRWAEYIRRLAASARPADYPSAAAYEMARSAFQDAEAFYRRLAE"
     gene            6244..6537
                     /locus_tag="VFA54_03165"
     CDS             6244..6537
                     /locus_tag="VFA54_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZS49834.1"
                     /translation="MNRTVFMGNLPAWVTAEDIQSWLQHENITYDDVKVIRDFETQES
                     KGYAFIEAATDEDSKAIIARFNHAPLEDRNLRVNEARGSDQGRKSQPRRSKAR"
     gene            complement(6650..7255)
                     /locus_tag="VFA54_03170"
     CDS             complement(6650..7255)
                     /locus_tag="VFA54_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZS49835.1"
                     /translation="MMRLLFACLLACGAWLRPAILAQRPGLVEVLVAYYSETGNTEKM
                     AQGVAAGASRVAGTHVTVKKIGSVTKQDLLRADAVVLGSPVHMGDAAVEVRRALVDWS
                     MKYEFFESKQLQDKVAGVFATGGAPSHGKELTMMSMAVSLLQLGMVLVSPYSGFGASA
                     TTATPEGKHGVADWELNEARELGERVAQVARKMKFGANANR"
     gene            complement(7332..8621)
                     /locus_tag="VFA54_03175"
     CDS             complement(7332..8621)
                     /locus_tag="VFA54_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HZS49836.1"
                     /translation="MIASIIQNSLDALQGYVKFPAAEVAGRIAVALGIGLAIGLEREW
                     AHKEVGVRTFAITALLGMLSSLLGPSFAVVAFVGVFSLIAYVNIRSLVVERTVEITTS
                     VALMAAVLLGVLAGEGHFFTAISSAIAVTMLLAWKAELSRFAVGLTVEEIRSAVFIGL
                     IAFVIYPLLPNRFVDRWSLLNPREIWIVILLLAGIGFTNYILLRLYGARGLYYTALLG
                     GLVNSTAAVAELSSWLDAIGGEMAGLGVSAILLALVAMFLRNLAIVAIFGVAAFQTAL
                     WPLGAMTLLAGGIVWACSRRNSGQAPAALRLTSPLAMRRICAFGILFVLIQICGNLAE
                     RHLGHLGLIAVSVIGGLVSSASTAATAAIMVGHRQLEPGVAGIAVVLCSISSSLVDIP
                     LVYHQIHDRGLRRNLLASLGAMALAGVIVLAVTWQRY"
     gene            complement(8769..9812)
                     /gene="cydB"
                     /locus_tag="VFA54_03180"
     CDS             complement(8769..9812)
                     /gene="cydB"
                     /locus_tag="VFA54_03180"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZS49837.1"
                     /translation="METAWFILVSLMLAGYVVLDGFDLGAGVLHLLAARSEEERRMIL
                     QSVGPVWDGNEVWLIAAAGTLFYAFPLLYAASFSGFYLALHIVLWLLVFRALGIELRM
                     HLANPVWRAFFDGTFSLASIWLTLLFGAALGNVIRGVPLGPDHYFFLPLWTDFRPGSQ
                     PGILDWYTLLCSLAALAAVAVHGALYLIMKTAGAVNARLRAIVKWLWPFLVALTVFTL
                     LATLHVRPDVIENYRTKPAGWLLPTSVALGLAGVFHFTRAGEEQNAFLSSCLYIVAAL
                     SGAAFALYPTLLPSSSDPANSITIRNAAAGAASLSLGLIWWGAGMAIAVGYSVIVYAM
                     FRGKVGAPAGGRE"
     gene            complement(9821..11188)
                     /locus_tag="VFA54_03185"
     CDS             complement(9821..11188)
                     /locus_tag="VFA54_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZS49838.1"
                     /translation="MDDAYFVHRIHFAFTIIYHYIFPQLTMGLAPLIVALKTLSLRSD
                     RPAFGHAARFWTKIFGINFLAGVVTGIPMEFQFGTNWARFSQWTGGVIAQPVAMEGVF
                     SFFLESAFLGLFLFGEKRLSRAAHWAAGFLVFLGSWLSGFFIVAANAWMQHPVAYKRL
                     PEGTFEVTSFWGLLGNPWALIQFAHTMSGAVVTGALVMSAVGAFYLLNGRHAEYGRIF
                     VCVGVIAGFAASVFQIFPSGDMHGRYMAHAQPVTTAAMEALFRTEAGAPMVILGQPNI
                     ERRQIDNPILVNKALSFLIYGTTRAEVQGLDHFPEQDWPTNIPLLFFSYHVMAGLGTL
                     FVAVTAVALGLLWKGALFETRWMLWILMLAFPFPYIANTAGWLTAEAGRQPWLVYGLV
                     RTQQGFSRNVSAANGLFTLLGFMGLYALLSILLLFLLERQIEQGPETESSEEYLPASI
                     SGGGL"
     gene            complement(11282..12199)
                     /locus_tag="VFA54_03190"
     CDS             complement(11282..12199)
                     /locus_tag="VFA54_03190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZS49839.1"
                     /translation="MQLARQGYYDRSAFHRVVANGFIQGGDPLLKNPKTPRNLWGTGG
                     LNLLPGEFSDWKHERGIVSTVHTPNKPNSDGSQFFICVSPQPALDGQYSAFGRVTEGM
                     NIVERISQAPTGADGLAQTPVRILKIAIEKKKEESFLDASPDQMRRTATLKTTLGSLK
                     IQMEPDWAPSHVRNFLKLAASGWYNGTAFHRIVKGFVVQGGMGETRSSGPYHPADRWV
                     RPLKGEFRKDVRHVRGIASMAHGEDPDSATTSFFLVLGPAPHLDGEYSAFGRVVEGLE
                     GLDAFEKEPVEGEKPVRRLEIVEAAIDPR"
     gene            12155..12334
                     /locus_tag="VFA54_03195"
     CDS             12155..12334
                     /locus_tag="VFA54_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49840.1"
                     /translation="MKCAPVIVALPGELDELINVLGSFVGRKFKTKAPSSVVTTASSS
                     AGGSEEGDCARTIAV"
     gene            complement(12529..12605)
                     /locus_tag="VFA54_03200"
     tRNA            complement(12529..12605)
                     /locus_tag="VFA54_03200"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12569..12571),aa:Met,seq:cat)
     gene            complement(12697..14289)
                     /gene="serA"
                     /locus_tag="VFA54_03205"
     CDS             complement(12697..14289)
                     /gene="serA"
                     /locus_tag="VFA54_03205"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZS49841.1"
                     /translation="MNILIAEALAPAAMELLRSEPGWTIIESNPKEFRAHLTEAEALI
                     VRSAAKVTAETLAQAPNLRVIGRAGVGIDNIDLPAATERGVLVMNTPGGNAVSVAEHT
                     LALMLSLARSIPQASASTRAGRWEKKRFLGNELRGKTLGVIGLGNIGVAVVRLARPFG
                     MKIIAHDPYVAPQIASDEGAELVPLDDLYARSDYITLHVSLTPQTNNMLNRAAFARMK
                     HGVRIVNCARGELIDAAALEEALASGKVAGAALDVFAPEPPPKDFPLFAQENLIATPH
                     IAGSTEEAQEIVSLRIAEQIREYLKSGVAVNAVNLPAVTAEEFRHLRPYVQLATRLGA
                     FAAQISAGNPRGVRLIYSGKLAEGNTNLIRNAALAGMLNQFLSQRANLVNAAQIAAAR
                     GWRIDEIRGARLPYADSISVVLATDQGESAVEGAVLLNDSPRLLSVDGIQVEAPLRGH
                     LVYMRNWDVPGVIGRVGTILGENKINIANFSLGRRENHARPADPADAVAVVHIDEPVG
                     EPVLDQLRRSPGVMFACAVELA"
     gene            complement(14286..15461)
                     /locus_tag="VFA54_03210"
     CDS             complement(14286..15461)
                     /locus_tag="VFA54_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZS49842.1"
                     /translation="MLIRKTRLLTPGPTPLYPPATRAMLASDLHHRTEEFRRLYKGVI
                     EDLKYFMGTRNDVLLFTSSGTGAMESAVSNLFSRGERVVCVSAGKFGERWIEIAKAFG
                     LATTILSAPYGEAVLPARVAAAVRDEPSIRGVLVQATESSTGVAHDIEAMAAAVRGTD
                     VLLIVDAITGLGTSVLDIDGWGLDVVIGGSQKALMVPPGMAYLSVSPRAWERARRSDL
                     PHYYFDLRKEKQSGDKGESSWTPATSIVLALAEALRYIREIGRENLIQNAQLLARATR
                     AAVAALGLRLFASRSPAGALTAVLAPEGMDSGVIVKEFRESFGAIVANGQGEMKGKIF
                     RIAHLGYFDFADLFAVIAELEVILHRLGLPVRLGAGVAAAQQVYIEAGQQQPALAVR"
     gene            15612..16058
                     /locus_tag="VFA54_03215"
     CDS             15612..16058
                     /locus_tag="VFA54_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZS49843.1"
                     /translation="MAFQDQLQKDIQQAMRDRDEERLSALRMMKSAIKNKEIEIMKPL
                     DDAACFQVLATLIKQRRDSAEQFRKGNRPELAAKEEREIQVIESYMPSAPSEEEMEKA
                     INEALAETGIASLKQMGVVMKAAQAKLAGRRVDGKILSERVRARLS"
     gene            16067..>17545
                     /locus_tag="VFA54_03220"
     CDS             16067..>17545
                     /locus_tag="VFA54_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HZS49844.1"
                     /translation="MATTQQQIRHQPYVPEAAQLREFTLRAVLIGLVMTVVLGAANAY
                     LGLRAGMTVAATYPAAVISMAILRARRGTILEENIARTAGSIGESVAAGAIFTIPAFL
                     ISRSWPSFDFQHAYWKSTALMLVGSVLGVLFVSLVRRVMVEDPELPFPESVAAGEIHK
                     AGQRGGESAKFLFYNAAVGAAVYLLGVFQLFAADKDRVIAVGDLGRSSVRLGAGGNSP
                     SLGVGGFTLVSAPSVSPAYLGVGFIIGPRLAALNFAGSVLAWGLLIPMLIYFLGPQLQ
                     RYLPPGAAADSWAGLAGAAWRFIVRPIAVGGMIVGAAYTLFRMRRNLALALGKTLGEL
                     RAPASEIKARGRLDQYMSSKSVMGLIGFMFLLMIALYVYFSGRFMAGVIAAAVMLVVG
                     FFFSTISGSLVGLIGSSNNPISGLTLCTLIIAALLMVSLGVSGSPGVVTVLGVAAVVC
                     VAAAVAGELLQDFKVGYILGGTPRSIQIVELIAVTLASLVMYF"
BASE COUNT         3517 a         5395 c         5403 g         3230 t
ORIGIN      
        1 gacctgatcg ttctggacaa aagccctctc gatgatattc gcaataccag agcaatcgcc
       61 gccgtctatc tgggcggcca gaaagtccgg tagatcctag cgttgctccg acggcgccgc
      121 cgagcgggtt tccgtcaggc ctgacaggtc ttcaaactcg acgcggtttc gcccgccgct
      181 cttggcacgg cgcacggcga cattgaccgc ctgcaaaaga aggtcggcat ccggattggg
      241 cgcgtcttcg gtggaagcca cgcccacgct cgcggtgatg gaaatgctgg cgcccagcag
      301 gtcgaccggc tcatcgctga tgcaagcgcg cagcttttcc gcttgctcca aagcctccga
      361 ggaatagcag ccgggcacga cgaccaggaa ctcagcgctg gcataacgtc cgatcgagtc
      421 gtacacgcgc acggcggagc gcagccggcg ggcggcttcg cgcagcacgc tctcagccgc
      481 ggtgggcccg tgattcatgg cgacttgccg gaagcggtcg agatggacca ggatcaggcc
      541 gaccggcgcg gtttcgcggc gctcgcgcga caccttttgc cggagcatgc ctaaaacggc
      601 ttcccggttc cacactccgg tcaggaaatc atgcgtggcc gggaagcgcg ccgcctctcg
      661 catcgacaga agatgttctt gcagatcgag aatgcggcgg cccgcccgca agcgaacccg
      721 cagctcgtcc gggtcgaagg gcttagtcag gtagtcgtcg gcgccggcct cgagtccctc
      781 cacgacatct tccttctggg acttggcagt cagcagaagg atgtatacgt agggatggcc
      841 ggtgcgcttg cgtacttcac ggcagagctg aagtccatcc atgcccggca tcatccaatc
      901 caggatggcc aggttgggcg aatcgtctct ctgcaacgtc caccaggcct catcaccgtc
      961 gttcgcgaca accgtctcgt acccccactg caccaagaat ttttgcagca gatgccggct
     1021 gattggttcg tcttccgcaa tcaatattct cattatgcaa gctccttatc gagcgtgatc
     1081 aactcgggtt ccagccgcgc gatttcccgt tccaacgcgg catataactc ttgagccttt
     1141 tccaagtccc cctggcgcgc cgccatttcc agttggcgcg ccgcctcgga ggcggccaga
     1201 gcagaaaggt tgctgagcga gcctttgaag gtgtgcgcgg cggtctcgag cgcctttgta
     1261 tcccgctcgg cgactgcttt ctcgattttt aacaaaaatt tcggacattc ggcccgaaat
     1321 aagccggcca gggttctaac caactcccaa tcgccttgag cgcgcgccag caattcgcgc
     1381 gcgttcagcg agctatgccc attgcgtcct ttgaccgcat cggggctgga ggagccggag
     1441 cttgcagccg gccggcccgg cgtggcgggc agtttcatca catttccttt cggttgcgaa
     1501 cgcgcgccgg cggacttggc cgggacgagt ttctcgatag tggaaaacaa ctccgccgca
     1561 tcgataggct tggatatgta ggcgttcatt ccgctctcca ggcagcggtc tttgtcgcct
     1621 ttcatcacat gagccgtcat ggccacgatg ggagtgttcc tgccggtcag cttttccctc
     1681 gcgcgaatgg ccatggtggc ttcaaagccg cccatttcgg gcatctgcac gtccatgagc
     1741 acgagatcaa acggctcggc ttccagcgcc gcaatggcct cgcggccatt gccggccacc
     1801 accaccgtgt ggccgcgctt ttccagcagg cggcgggcca gagtttggtt gacgggattg
     1861 tcctccgcca gcaggatgtg cagcgaaaca gcggccgtgc cgccaatggg ggcgagcgtg
     1921 gcttccgccg gcgccatgtc gagagctttc atgagggaaa ccaaaaggct cgaaggcctc
     1981 accggtttgg acacacaggc cgcgataccg agatccgggc ggcgggcggc gtcgccgacc
     2041 tggcccgctg gagaaagcag cagcagtttg ggggatccga actcgggacg atcccggatc
     2101 ctctcggcca gcgtgattcc atccacctcg ggcatctgcg cgtcaatcag gatcaggctg
     2161 aaaggatctc ttttctcgac cccggcagcc agcgcagcga gcgcctcgtc ggcgctgccg
     2221 gcggcttgag gcctcatgcg ccacccggct aagatttccg cgaggatctg gcccgcagtc
     2281 gcgttatcgt ccaccacgag cacggcgagg ccttgaagcg aggcggggac cggcttcgca
     2341 agctgccgcg ggtcctcggc cgccccaagg ttagctgtga aatggaacgt gctgcccttg
     2401 ccgggctcgc tttccaccca tattttcccg cccatgagct gaaccagacg ggacgaaatg
     2461 gtgagcccca agccggtgcc gccgtatttc cgcgtggtgg aggtgtccgc ttgctggaag
     2521 gcttcgaaaa tggcctgctg cttgtccttg ggaatgccta tgcccgtgtc ggttacggca
     2581 aagtgcagga agatcggatc ggccttgccc gcggactgct gctccgccaa acccacgtga
     2641 accactactt cgccccgttc tgtaaacttg atcgcattgc ctaccagatt gatgaggatc
     2701 tggcgcaggc gccacgggtc gccgacgagc gtttcgggca cgtcagcggc gacgcgatag
     2761 gccagctcga tgcccttctg ttggccgcgc agggccacgg cgccgagcgc atcgccgatg
     2821 gagtcacgga gattgaaagc gaccggatcg agctcgagtt tccctgcttc gattttagag
     2881 aaatcgagga tgtcgttgat caccgccagc agagcgtcgg ccgaggattt caccgcctgc
     2941 aagcattccc gctgttcagc ggtgagatcg gttcccaggg cgagctcggt catgccgata
     3001 atgccgttca gaggagttcg aatctcgtgg ctcatgttgg cgacgaactc gctcttcgcc
     3061 cggctggcgg cttcggcggc ctctcgggcg cgctgcgact cttcttcggc gcggcggcga
     3121 tcggtaatgt cgagcagagc gcagcgcatg cctgcgatct cgccctccgc gccccgaatg
     3181 aagttctcgt gaatctccac ggtcagccgc gcgccgtcgc ggcggacgta ttcgcggcgc
     3241 acaggagcga gcggctcctg gccggcgatt tttcgccgca cggtccgttc actttcctcg
     3301 cgctgctcgc gggccacaaa ctgccacgcc ggtttgccga gaatctctga ggccggaaag
     3361 ccgagcaaag cgcattccgc ctgattcacg cggcggagta tgccgtcgcg gtcgatctcg
     3421 tgatacgcga tcggcgcttc ctcgaaaagg ctcctgtatc tctcttcgcc ttcccgcaaa
     3481 gcttcctggg cgcgtttgag accggtgata tccaccatgt aaatcaaaat ccggtcgcgg
     3541 cgcgccacgt aacagatttt ttgctgaaaa atggcgccat ttacctgaat ttcccgcgcc
     3601 atcgagtcct ctactcccca acgaaacgat tggattatgg gggccaaatt ttccagaatc
     3661 gggtgatccg ggccggaggc ctccagatcc ggaaattgac gctgagcggc gggatttaca
     3721 taggtcacat tgccggccag atcggcctct acaaacggat tggggttctg gctggggaag
     3781 gacgcggcaa gcaccagttt ttcttccgcc tgcttgcgct ccgcgatctg gccagccaac
     3841 agacgccggg cttcttccag ttcgatcggg cttcgcaggg ccagcgcctt tgggatcacc
     3901 ggcaggaaca ggagggccgc tacgagcgaa acgccggctg ttacagcctc gagcggagcc
     3961 tggaactgtt cacctccgat ctgcgccaga tgcagggcgc cgcaggcgat cagaaacacg
     4021 gcgaaaaggt ggagcacccc tcgaaacggc atgtcccgcc tcttcccggc gaaatagagg
     4081 agcgaagccg acatgacgca gcacgagacg gcaatggcga gattggacaa attgcgcgcc
     4141 cagggggcaa aggaaactgc tatgcaggcg aaaggcgcga actttccatc cagcagcatc
     4201 tccataagcc tgttacgcgt aaagggccat ctggcacagg cggcgcgcgc ggtcttggcg
     4261 gtcaggccgc ggcagcggag tttgggggga gcagccggcg ctcgagccgc ctctccgccc
     4321 ggcgccgggc gacagctcac gtatatcatt gagaggcata tatctggcgc cgaaaaagag
     4381 aaccgccgac atgtaaaccg gaaagtccgg ctaatatggt agtgcatccg tcgagtcagc
     4441 cagaacagac agacacctta gtccggacgc ggcgattctc cgaaatgagc tacaacaaca
     4501 acctgagcgt ggtgggcggg aagtagtagc ctgttagtgg taatctggtc gaaccatgct
     4561 gctgcgctgg cgcctgcttg ccttggccgc aatgtttccc gccgccgccc agcagccctc
     4621 taccggcgct cttgacatct atataaccag cggcgggaaa ccggcggcag ctcgggttta
     4681 tgtgtgggac gcaaacgggc ggccgctgct gattccaggc gcagtgacgt attcgctccg
     4741 cgaggaaagc cactctgtca tcgaccagat ggcgaccgcc atgttgccgc cgggcaaata
     4801 ccgggtgcgc gccgaaaagg gcatggaatt ccaagccgct gagaaaaccg ccgccgtgac
     4861 cccgggcgaa gcggcgcgga tcgacctcga agtctcccgc ctctacaaca tgaacgggca
     4921 aggatggtat tccggagacc tgcacattca ccggagccct gaggatatgc cgctcttgct
     4981 ccgcgccgag gatctcaacg tggccccggt gattacccgc catttgggaa gcggaacaga
     5041 ggaagggccg ccgttcaccg aaactccctt gcggagcgtg gatcagaacc acatcgtcag
     5101 cttccagaat caggaagtgg aacggctggg caaagggcat ggagccattc tcttgctgaa
     5161 tactaggcgc gccatagatt ccaacatggc gcctttgttt cccccggacc gcgatttcgc
     5221 ccgcgaggcg agagcccagg gcggtttcgt ggatgccgag aagccgatct ggaaaaacgt
     5281 tccggtgaac ctcgcgttcg gcctcgtcga ctcggtggga gtggtgaaca atcacttcca
     5341 tccgcacggc gttctgctcg aagcggagaa atacggctcg atggagcgcg agggagcgat
     5401 ctacaagggc gccgccggtt tcgctcagtg gatgatagac ctctattaca gcttcctcaa
     5461 ttgcggcttt cgcctcgcgg tatcggccgg ctcggcttcc ggcattatgc cgagctggcc
     5521 cggctatgag cgaacatacg tccatctgag cggacctttc agctatgaac agtggtttcg
     5581 ggatcttcag gcgggccgga gcatcgccac gaacggacct ttgctcgagg tttacatgaa
     5641 tgggcagcct cccggcgcgg ccgtggattg ggaagctcct gccggtgtga cattagccat
     5701 cgacgcgcat gcacaggaga gattggaccg cgtggagatt gtctacaacg gcgaggtgat
     5761 ccgttccttt tccgccgggg gcaacaccgt tttccgaacc gccctgaacc tcaccatcac
     5821 cgagcccggc tggcttgccg tgcgctgctt cgagccggtt cgggaaaccg tgcgttacgc
     5881 ccatacatcg ccgttttact ttctgcggga ggggaagctg ccggtgaagc ggcccgcggc
     5941 gctgcggtgg gccgagtata tccgccggct ggctgcgtcg gcgaggccgg ccgactatcc
     6001 ctcagccgcc gcgtatgaaa tggcccggtc ggcctttcaa gatgcggagg cgttttaccg
     6061 gcgcctcgcg gagtagagcg ggctggcggc gaaattggtt aatatggatg agtcagcatg
     6121 cctgcttcag cgcccgagca cagggcggtc ggcctgccgg atagcgcgtt gccgggatgg
     6181 ccaccggccc gggcttggaa taggttataa ttcgtgtcta cttcaatggc taagaaaggg
     6241 tcaatgaaca gaaccgtgtt catggggaac ttgcctgcct gggtgaccgc cgaggacatc
     6301 caaagctggc ttcagcatga aaacatcacc tacgacgacg taaaggttat ccgcgatttt
     6361 gagacgcagg agtcgaaggg ctacgctttt attgaagcgg ccacggacga agactccaag
     6421 gcgatcatcg ctcgcttcaa ccacgccccg ctggaggacc ggaatctgcg agtcaatgaa
     6481 gctcgcgggt cggaccaggg acgcaagtcg caaccccgcc gctcgaaagc acgctaggga
     6541 tccagcattt cctccatccc acggcccttg tgtcgtatgg gactgcgtgt gtgcgcctgc
     6601 ccctgcgggc aaagccacac ggccgcgtcc cagtccaggg aggcggctgt catctgttgg
     6661 cgttggcgcc gaatttcatt ttgcgagcga cctgcgctac ccgctcgccg agctcacggg
     6721 cctcgttcag ctcccagtcg gctacgccgt gcttgccttc cggcgtggcc gttgtggcgg
     6781 aagctccaaa gccggaatag gggctgacca agaccattcc caattgcagc aaagaaacgg
     6841 ccatgctcat catggtcagc tctttgccgt gggacggggc gccgccggtt gcgaaaaccc
     6901 ccgctacctt gtcctggagc tgtttgcttt cgaagaattc gtatttcatg ctccagtcga
     6961 ccagcgcgcg gcggacctcg acggccgcat cgcccatgtg aaccggcgat cccagcacta
     7021 cggcgtcggc gcgtaacagg tcttgcttcg tcaccgaacc tatcttcttc accgtcacat
     7081 gagttccggc cacacgcgac gccccggcag ctacgccctg ggccatcttc tcggtgttgc
     7141 ctgtctcaga gtagtaggcg acgaggactt ctaccaagcc cggccgctgc gccaggattg
     7201 ccggccgcag ccatgctccg caggccaaca agcaagcgaa aagcaaacgc atcatcggcg
     7261 gactcttctg agacagattg agagcgccgc ccgttgcgat gggtggcaac atttggaggg
     7321 agctccctta ctcaatatct ttgccacgtc accgccaaga caatgacacc tgccagcgcc
     7381 atcgcgccaa gagacgccaa aagattccgt ctcaagcctc tatcatgaat ctgatgatag
     7441 accaatggga tatccaccag cgagctggag atggaacaca gaacaacggc gatgcctgcg
     7501 actccgggct ccaattgccg atggcccacc atgatggcgg cagtcgcggc cgtactggca
     7561 ctgctcacaa ggccgccgat cacgctaacg gcgatcaaac ccaaatggcc gagatggcgc
     7621 tcggccagat ttccgcaaat ctggataagg acgaatagaa ttccaaaagc acagatcctg
     7681 cgcatggcga gaggcgaggt caatctgagg gcggccggag cttgcccgct gttgcgccgg
     7741 gaacaagccc agacgatgcc gcccgcgagg agcgtcatgg cccccagagg ccaaagcgcg
     7801 gtttggaacg cggcaacccc gaagatcgcc acaatcgcca gatttcttaa aaacatcgcg
     7861 accaacgcca gcaaaatggc cgaaacgccg agtcccgcca tttcccctcc aattgcgtca
     7921 agccacgagg aaagctccgc aaccgccgct gtgctgttta ccagaccgcc gagcagagcc
     7981 gtgtagtaaa ggccccgcgc gccgtagaga cgcagcagga tgtagttggt gaaaccaatc
     8041 ccggccaaca gcaggattac gatccaaatc tcgcgcgggt tgagcaggct ccagcgatcg
     8101 acgaatcgat tcggcagaag cggataaatg acaaaggcaa tcaggccgat aaagacggcg
     8161 ctgcgaatct cttccacggt gagccccaca gcgaacctgg agagctccgc tttccacgcc
     8221 aacagcatcg tgacggcgat tgccgaagag atggcggtga aaaaatgccc ctcgccggcc
     8281 agaacgccca aaagcacggc agccattagc gcgacagagg tcgtgatttc cacggtccgt
     8341 tccaccacca ggctgcggat gttcacgtaa gcaataaggg aaaaaacgcc cacaaatgcc
     8401 acgaccgcga agctgggacc gagcagggag ctcagcatgc cgaggagagc cgtaatggcg
     8461 aaggtccgga cgccgacttc cttgtgagcc cattcgcgct ccaggccgat agccaggccg
     8521 atgcccaggg cgacagcgat tctgccagcg acctcagccg ccgggaattt gacgtagccc
     8581 tgaagggcat cgagagagtt ctggatgatc gaagcaatca tgagagatca gcgcaggcgc
     8641 cgcggcaatg gacgggcatt cggacatccg aacgtattcc cgcgaaacta gggaagcggc
     8701 ctgtttcccg tgcgaagcgc gtggtcaggg gtggttggct ccgatctggg ggaccggggc
     8761 gggcggtttc actccctgcc gccagccggt gcgccgacct taccccggaa cattgcatat
     8821 acgatcacag aataaccaac ggcaatcgcc attccggcgc cccaccaaat caggcctagc
     8881 gagagagagg cggcccccgc tgcggcgttc cgtatcgtga tgctgttggc cggatcgctg
     8941 gaggatggca gcagagtcgg atacaacgcg aacgcggctc cggaaagcgc cgccacgata
     9001 tagaggcaag aagacaaaaa tgcgttctgc tcctcgccgg cgcgagtgaa atggaaaacg
     9061 ccggccagac ccaatgccac actggtcgga agcaaccagc cggccggctt ggtccggtag
     9121 ttttcaatca cgtccggtcg gacgtgcaac gtcgccaaca gcgtgaatac ggtaagcgcg
     9181 accagaaacg gccagagcca tttcacgatc gcgcgcaggc gagcgttcac cgctccggcg
     9241 gtcttcatga tcagataaag cgcgccgtgg accgctactg ccgccagggc ggccagggag
     9301 caaagaagcg tataccagtc caatatgccg ggttgcgagc caggccggaa gtcggtccag
     9361 agtggaagga agaagtaatg atcgggaccc agagggacgc cgcggatcac gttgcccaat
     9421 gcagctccga aaagcaacgt gagccagatg ctggccaggg aaaatgttcc atcgaagaag
     9481 gcgcgccaga ctggattcgc caaatgcatc cgcaattcga tgccgagcgc ccggaacacc
     9541 agcagccaaa gcactatgtg caaggcgaga tagaatccgc tgaagctcgc ggcgtagagc
     9601 aggggaaagg cgtaaaaaag ggtcccggcg gcggcgatca gccacacctc attcccatcc
     9661 cagacgggcc cgaccgattg caggatcatc cgccgctcct cctcgcttcg ggccgccagc
     9721 agatgcagca cgcccgctcc cagatcgaag ccatccagca ctacgtagcc cgccagcatg
     9781 agcgagacga gaatgaacca ggccgtctcc atccgtgctc ctacaaaccg cctccggaaa
     9841 tggaggctgg aaggtattct tcgctggatt cggtttcagg gccctgctcg atctgccgct
     9901 cgagcaaaaa tagcaagagg atcgaaagca gggcgtacag ccccataaat cctagcagag
     9961 tgaaaagtcc gttcgcggcc gaaacattgc gagaaaatcc ctgctgagtt ctcaccaggc
    10021 cgtagaccag ccagggctgg cggccagcct cggcggtcag ccacccggcg gtattggcga
    10081 tataggggaa ggggaacgca agcattagga tccagagcat ccagcgggtc tcgaacagcg
    10141 cgcctttcca gagcagcccc agcgccacgg ccgtgacagc gacaaacagc gttcccagcc
    10201 ctgccatcac gtgataggag aaaaaaagca ggggaatatt ggtgggccaa tcctgctccg
    10261 ggaagtgatc gaggccttgg acctccgccc tggttgtacc gtagataagg aagctgaggg
    10321 ccttgtttac taatatcgga ttatcgattt gcctccgctc aatgtttggc tggccgagga
    10381 tgaccatagg cgcgccggct tcggtgcgaa aaagcgcttc catggcggcg gtcgtcactg
    10441 gctgggcgtg agccatgtag cgcccatgca tatccccgga agggaaaatc tgaaagacgc
    10501 tggcggcgaa accggcgatc acgccgacgc aaacaaatat gcgtccgtat tcggcgtgcc
    10561 ggccgttgag aaggtagaac gcgccgaccg ctgacatcac gagcgcgcct gtgaccacgg
    10621 cgccgctcat ggtgtgagcg aattgaatca gagcccacgg atttccgaga agaccccaaa
    10681 agctggttac ttcgaaagta ccctcgggca ggcgcttgta ggccacggga tgctgcatcc
    10741 aggcgttcgc cgccacaatg aagaagccgg aaagccagga acccagaaaa acgaggaatc
    10801 ccgccgccca atgggccgca cggctcaagc gtttttcgcc aaaaaggaac aaaccgagga
    10861 aagccgattc tagaaaaaaa gaaaagacgc cttccatggc gaccggctgc gcaatcactc
    10921 cgcccgtcca ctgcgagaac cgcgcccagt tcgtgccgaa ctgaaactcc atggggatcc
    10981 cggtgaccac tccagccaga aaattgatcc cgaatatttt ggtccagaac cgcgccgcat
    11041 ggccgaaggc gggacggtcg cttcgcaagc tcagagtttt gagcgccaca atcagcggcg
    11101 ccagacccat cgtcaactgc ggaaagatgt agtgatagat gatcgtgaaa gcgaagtgga
    11161 tccggtgaac aaaataggcg tcatccatga cgtaaatgaa accggcgccc gggcgctcct
    11221 agcgaatcta tgcttccgcg ccgccctcat ctcctatgcc ggcggcggaa atccgggcgc
    11281 ctcaccgggg atcgatggcg gcttcgacaa tttcaagccg ccgcaccggc ttttcgccct
    11341 ccacaggctc tttctcaaag gcgtcgagcc cttccaggcc ttcgacgacg cgcccgaacg
    11401 cggaatattc gccatcgagg tgcggcgccg ggccaagcac caggaagaaa gaggtggttg
    11461 ccgaatccgg atcttcgcca tgcgccatgg atgcgatgcc gcggacgtgc cgcacgtctt
    11521 tgcggaattc gcctttgagc gggcgcaccc agcgatcggc aggatgatag gggccgctgg
    11581 agcgtgtttc tcccatgccg ccttgaacca caaatccctt tacgatgcgg tggaacgccg
    11641 tgccgttata ccatcccgaa gccgcgagct tcagaaaatt tctgacgtgg ctgggagccc
    11701 aatcgggctc catctggatt ttcaggctgc cgagcgtggt cttgagagtg gcggttctcc
    11761 gcatctggtc cggcgaagca tcgagaaagg attcctcttt tttcttctca atcgcgatct
    11821 tcagaatccg gacgggcgtt tgcgccaggc cgtctgcccc ggttggggcc tgcgagatgc
    11881 gctcgacaat gttcatacct tcggtgacgc gcccaaaggc cgagtactgg ccgtccaaag
    11941 ccggctgggg cgaaacgcag ataaaaaact ggctgccgtc gctattcggt ttgttggggg
    12001 tgtggactgt ggagacaatg ccccgctcat gcttccagtc gctgaactcg ccgggaagca
    12061 gattgagacc gcccgtcccc cagagatttc taggtgtttt cggattctta agcagcgggt
    12121 cgccgccctg gataaacccg tttgccacga ctcgatgaaa tgcgctcctg tcatagtagc
    12181 cctgccgggc gagctggatg aactgatcaa cgtgctgggg agctttgtcg ggcgcaaatt
    12241 caaaacgaaa gctccgagtt cagtggtcac gacggcctcg agttccgctg gcggcagcga
    12301 agagggcgat tgcgcgcgga cgatcgccgt gtagagcagc agtgcaagta gataacgcgg
    12361 catcgaccag ccatattatc ccaatttcgc ccaccgaccg cggccgtgcg gccggttcgc
    12421 tgattgtgat gccttccact gtcgccgcgc tcacagcggc ttacgcccgc aggtttttac
    12481 aggagagact atctgatctg ggtaaagtat tggaaatcgg aagggttgtg gttgcggggg
    12541 gtggatttga accaccgacc tttgggttat gagcccaacg agctaccaga ctgctccacc
    12601 ccgcgcattc atcgtagcgc agcacgagcg cggagtcaaa tgacgatgcc gatcgcatca
    12661 gaccatctcg gcctgagagc aagttcacac gaagccctac gccagttcca cggcgcaggc
    12721 gaacattaca ccgggcgaac ggcgcaactg gtcgaggacc ggctcgccga ccggctcatc
    12781 aatatgcacc acggccacgg catccgccgg atcggcgggc cgcgcgtgat tctcccgccg
    12841 gcccagagaa aagttggcga tgttgatctt gttttcgccc aaaatcgttc caacccgtcc
    12901 gatgactccc ggaacgtccc agttcctcat atagacaagg tggccccgca gcggcgcctc
    12961 cacctggatt ccgtccacgc tcagcaggcg cggcgaatcg ttcagcagca cggcgccttc
    13021 cacggcggat tcgccctgat cggtggcgag gaccaccgag atggagtcgg cgtaggggag
    13081 gcgcgctccg cggatttcat caatacgcca gccgcgtgcg gcggcgatct gcgcagcgtt
    13141 caccagattg gcgcgctgcg aaagaaactg gttcaacatc ccggccagcg ccgcgttgcg
    13201 gatcaggttg gtgttgccct ccgccagctt tccggagtaa atcagccgca cgccacgcgg
    13261 attgcccgct gagatctgcg ccgcgaaggc ccccaggcgc gtcgcaagct gtacgtaagg
    13321 acgcaaatgg cgaaattctt ccgccgtcac cgccggcagg ttgacggcgt tgaccgctac
    13381 gccgcttttc aaatactccc ggatttgctc ggcgatccgc aggctcacga tttcctgcgc
    13441 ctcttcggtc gagccggcga tgtgcggcgt ggcgatcagg ttctcctgcg caaacagcgg
    13501 gaagtctttc ggcggcggct cgggagcgaa gacgtccaga gcggcccccg cgacttttcc
    13561 cgaggccagg gcttcttcga gcgctgcggc gtcgatcaac tcgccccggg cgcagttcac
    13621 gatccgcacg ccgtgtttca tgcgggcgaa cgcggcgcga ttcaacatgt tgttggtttg
    13681 cggggtgagc gatacgtgca aggtgatgta gtcgctgcgg gcgtagagat catcgagcgg
    13741 aaccagctcg gcgccttcat cggaagcgat ctgcggagcc acatagggat cgtgggcgat
    13801 gattttcatt ccgaacggcc gcgccagccg caccacggca acgccgatgt tgccaagacc
    13861 gatcaccccc agcgttttgc cccgcagttc gttgcccaaa aaccttttct tttcccaccg
    13921 gccggcgcgg gtcgaagcgc tggcctgcgg aatggaccgg gccagggaca gcatcagcgc
    13981 cagcgtatgt tcggcgacgg aaacggcgtt gccgcccggt gtattcatca ccagcacgcc
    14041 gcgctccgtg gcggccggca gatcaatatt gtcgatacct acgccagcgc ggccgattac
    14101 ccgcaggttc ggcgcctggg ccagcgtctc tgcggtcact ttcgccgcgc tgcgcacgat
    14161 cagggcttcg gcttcggtca ggtgggcgcg aaattccttg gggttcgatt cgatgatggt
    14221 ccagcccggc tcggaacgca gcaactccat agcggccggg gccagcgctt cggcaatgag
    14281 gatgttcatc ggaccgcgag ggccggctgt tgctggccgg cttcgatata cacctgctgg
    14341 gcagccgcca ccccggcgcc gagccggacg ggaagaccga gcctgtgcaa aataacttcc
    14401 agctccgcga tcacggcgaa cagatccgca aaatcgaagt agccgagatg agcgatgcgg
    14461 aaaatcttgc ccttcatttc gccctggccg ttggcgacga tggccccgaa gctctcgcga
    14521 aactctttta cgatcacgcc ggaatccatg ccctcggggg ccagaaccgc cgtcagcgcg
    14581 cccgcaggag aacgggacgc gaacagacga agaccgagcg cggcgactgc ggcccgcgtg
    14641 gcgcgcgcca gcagttgggc attttgaatc agattttcac ggccgatctc gcgaatgtag
    14701 cggagcgctt cggcaagagc caatacaatg gacgtggcag gggtccaaga gctctcgccc
    14761 ttgtctccgc tctgtttttc cttgcgaagg tcgaagtaat aatgaggcag gtccgagcgc
    14821 cgggcgcgct cccaagccct cggactcacc gaaaggtaag ccatgccggg cgggaccatc
    14881 aacgcctttt gagatccgcc gatcaccaca tcgaggcccc aaccgtctat atccagaacg
    14941 ctcgtgccca gcccggtgat cgcgtccacg atcagcagga catccgttcc gcgcaccgcg
    15001 gcggccatag cttctatatc gtgagccact ccggtcgaac tttctgtagc ttgcaccagc
    15061 acgccccgaa tcgacggctc gtcccttacg gcggcagcga cccgcgcggg cagcacggct
    15121 tcgccatagg gagccgagag aatcgtcgtg gcaaggccga aagccttggc gatctctatc
    15181 cagcgctcgc cgaatttgcc tgctgagaca caaaccaccc gctccccgcg cgaaaacaga
    15241 ttcgaaaccg cgctttccat ggctcccgtg cccgaggacg tgaacaacag cacgtcattg
    15301 cgcgtgccca tgaaatactt cagatcctcg attacccctt tgtaaagccg gcgaaactcc
    15361 tccgtgcggt ggtggagatc cgaggccagc atcgcccgcg tggccggggg gtaaaggggc
    15421 gtgggtcccg gggttagcag gcgtgttttt cggataagca taagtgagag gccccgaacg
    15481 ccggcgctct gcggaggggc agtgtttact gcgccccgct cgcctggaat atcggatcgc
    15541 tgacgtccag cgcctaaaca tactagaata gcggcttccc ccgcgcggga gcaaactgtc
    15601 cggaggctgg cgtggcgttt caagaccagt tgcaaaaaga cattcagcag gccatgcgcg
    15661 accgcgacga agagcgcctc agcgccctgc gcatgatgaa gagcgccatc aagaacaaag
    15721 aaatcgaaat catgaagccg ctggacgatg cggcctgttt ccaggtcttg gccacactga
    15781 ttaagcagcg ccgggactcg gctgagcagt ttcgtaaggg caaccggccg gagctggcgg
    15841 caaaggaaga gagggagatc caggtcatcg agagctatat gccctcggcg ccgagcgaag
    15901 aagaaatgga gaaggcaatc aacgaggctc tggccgaaac cggcatcgcc tcgctgaaac
    15961 aaatgggggt tgtcatgaaa gccgcccagg ccaagctggc cggccggaga gtggatggaa
    16021 agatcttgag cgaacgggtc cgggcccggt taagctgagg gcagccatgg ccacgacgca
    16081 gcagcagatc aggcatcagc cgtatgtgcc ggaagcggcg cagttgcggg aattcacgct
    16141 tcgggccgtg ctgatcggcc tggtgatgac cgttgtgctg ggagcggcaa acgcgtatct
    16201 ggggttgcga gccggcatga ccgtcgcggc cacgtacccg gcggcagtga tctcgatggc
    16261 gattctgcgc gcccggcgcg gcacaatcct cgaggaaaac atcgcccgca ccgccggctc
    16321 gatcggagaa tctgtggcgg cgggtgcgat cttcaccatc ccggccttcc tgatttcccg
    16381 ttcctggcct tcttttgatt ttcagcacgc ctattggaag tccacggccc tgatgctggt
    16441 gggctcggtc ctgggcgtgc tttttgtttc cctggtccgg cgggtcatgg ttgaggaccc
    16501 cgaactgccc ttccccgagt cggttgccgc tggagaaatt cacaaggccg gccagcgcgg
    16561 gggcgagtcc gcgaagttcc tcttctataa cgccgccgtc ggcgcggccg tttacttgct
    16621 gggcgtgttc cagctcttcg cggctgataa ggaccgggtt attgcggtgg gagacctcgg
    16681 caggagctcc gtgcggttgg gcgccggcgg caattcgcca tccctcggcg tgggtggatt
    16741 tacgctggtt tcggccccga gcgtcagccc ggcctatctc ggcgtcggat tcatcatcgg
    16801 gccaaggttg gcggcgttga atttcgccgg gagcgtcctt gcctggggac tcctgatacc
    16861 catgctgatc tactttctgg ggccgcaatt gcagcgctac ctgccacccg gcgccgcggc
    16921 ggactcctgg gcgggcctcg ccggcgcggc ttggcgcttc atcgtgcggc cgatcgcggt
    16981 cgggggcatg atcgtgggcg ccgcctacac gttgttccgc atgcgcagga atctggccct
    17041 ggctctgggc aagaccctgg gagaactgcg ggcgccggct tcggaaatca aggcccgggg
    17101 ccgcctcgat cagtacatga gctcgaaatc cgtgatgggg ctgatcggct tcatgttttt
    17161 gttgatgatt gcgctctatg tctatttttc aggccggttt atggccggcg taatcgccgc
    17221 tgcggtgatg ctggtagtgg gtttcttctt ctctacgatt tcgggcagcc tggtgggatt
    17281 gatcggctcc tcgaacaacc cgatttcggg cttgacgctt tgcacgctga tcatcgccgc
    17341 gctgctgatg gtgtctctcg gggtttcggg atcgccgggg gtggtgacgg ttttgggggt
    17401 cgccgccgtc gtctgcgtgg ccgccgccgt tgccggcgaa ttgttgcagg atttcaaggt
    17461 cggctacata ttaggaggta cgccgcggag catccagatc gtcgagctga ttgcggtcac
    17521 cctggccagc ctggttatgt atttc
//
LOCUS       DASREK010000053         6347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2010000, whole genome shotgun sequence.
ACCESSION   DASREK010000053 DASREK010000000
VERSION     DASREK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010000"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFA54_03225"
     CDS             complement(<1..273)
                     /locus_tag="VFA54_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZS49845.1"
                     /translation="MILTLTVNPAIDHTVTTDRISYEDRIYVRSSLEQAGGKGINAAQ
                     VIQGLNAPVLALAVCGGEAGRRFAELLYSTRLPVKLIPIRNSIRRNL"
     gene            complement(270..938)
                     /gene="cysE"
                     /locus_tag="VFA54_03230"
     CDS             complement(270..938)
                     /gene="cysE"
                     /locus_tag="VFA54_03230"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZS49846.1"
                     /translation="MFRAIKEQIDTVFKEDPAAKSTLEVLLCYPGLHAILLHRLAHRL
                     YVWRVPLLPRLISQFSRLLTGIEIHPGARIGRRFFIDHGMGAVIGETAEIGDDVLLYQ
                     GVTLGGTGKEKGKRHPTIGNHVVIGTGASILGNITIGERSKIGAGSVVVKSVPPHSTV
                     VGVPGRVVAIDGVSVKDEDDDILEHGRLPDPLEQAINGLHERVAQLEAELKALREEAA
                     RPIL"
     gene            complement(1113..2417)
                     /locus_tag="VFA54_03235"
     CDS             complement(1113..2417)
                     /locus_tag="VFA54_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS49847.1"
                     /translation="MSQPEADESSPHISLAAYYRLTRENPNFRRLWLAQIVSELGDWF
                     YSVAVYDLVLRLTGSAQLVGAVVLAQILPAFFLGPTAGAVNDRVSRKAVMITADVLRA
                     GIVLGMLAVRTREQLWLLYVLLIAEVILAAFFEPGRSAVIPNIVEERQVIAANALSAA
                     TWSFNLAVGAGLGGLAVRFFGREAAFVLNSLSFLASAALLAGMRFREPHLETHQRLQW
                     LDILGLGPVLEGVRYVRGDLKLMSLLFVKFGLGFVGANLVLLPLMGEREFGDPAGGVL
                     GMSVLFMSRGFGALVGPFIGGRFAGSDQRRMRIGLLAGFLTVGAFYILFSRALTLAAA
                     AACVIAAHSGGSLIWVFSTTLLQLNTEDRFRGRVFAADFGLNMLSASVSAYLTGFAVD
                     HGMALRTTALVTGLAMLLPATAWALAMLRWSRSPEVAPGRAS"
     gene            complement(2427..3410)
                     /gene="trpS"
                     /locus_tag="VFA54_03240"
     CDS             complement(2427..3410)
                     /gene="trpS"
                     /locus_tag="VFA54_03240"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZS49848.1"
                     /translation="MKPRLFSAMQPSGSLHIGNYLGALKNWTKIQYDYETIFGIVDLH
                     AITVYQDPAELRQKIEEIAAIYIAAGIDPEHCAIFVQSAVPAHVELAWILTCVTPMGW
                     LSRMTQFKAKAEQQESVGAGLFLYPVLMAADILLYNAAIVPVGEDQAQHLELTRDIAG
                     RFNSIYGETFVVPQTKLPSVGARVMGLDDPTVKMSKSAPGSMHAVALLDPPERIQKVI
                     GRATTDAKPSVDFEDLSPGVANLLAIFQGFTGWSDEEMRKHFSGMRYSNLKKEVADAV
                     AASLAPIQRKYREIIAQPGYLRDILQKGAERVTPIANSTLELVKKKTGLYT"
     gene            3499..4707
                     /locus_tag="VFA54_03245"
     CDS             3499..4707
                     /locus_tag="VFA54_03245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HZS49849.1"
                     /translation="MSIPVEIEKLVYGGQGLGRFDGRVVLAPFVLPGERVRVEPEKES
                     SHLITARTIAVETPAVWRTPAPCPYFGICGGCHYQHMPYERQLEAKRAILLETLARLG
                     KIEWSGPLEIIAAEPWAYRNRVQLRVRKQAGRFQIGYFEFGSHRLCPVEHCPISSPGI
                     NKALAALARIGKARRFPDFVQAIELFTNEREVQLNVLESSRPVARRFFDWCAAEIEGF
                     CRSDFLDYPAGPDIYRVSSRSFFQVNRFLASRLAEIAAGGERGNTAVDLYAGVGLFSL
                     SLARQFSRAVANDSNRSACLDLVYNAERAGVAIEVQQAPADQFLEGFTEKIDFLLADP
                     PRAGLGNAVTASIARLRPRRLTIVSCDPSTLARDLMRLLAAGFKIISMKIVDLFPQTF
                     HIETVVTLAG"
     gene            4769..>6347
                     /locus_tag="VFA54_03250"
     CDS             4769..>6347
                     /locus_tag="VFA54_03250"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZS49850.1"
                     /translation="MRYPLAAPMAAVAAGILAAQYATFSFRELSTSILLLLALALAGL
                     LRGALRAGAAACLLGFSGVGALLASLPHPADRTRIDLVAEREHLDLRDPIRLAGWVRV
                     PPTVRRDRHQFVLAVESIGAGTAASGGVRVTILRRTGEAEPELHYGDHLEFLARLRHI
                     RNFKNAGSFDRAAALLRQDIEMTASVRAGVPLRKIEGRRGRPFPALIWRAREWVERRL
                     DRLLGPESRSGGIVKAMLLGDEAYIDPALSLAFQRTGTYHALVVAGLHVGALACLFLF
                     LFRSLRISGGWSAVATAAPLAGFVLLCGSRLPTVRAACMVTGYLIARAFYRHRRALNI
                     LAGTALGFLIVDPADLFDVSFQLSFLSVGAIAAIAVPVIERFLDPYRLALVDLPDRSR
                     DLHLPPKVAQIRIEWRAIAARLPFAQRNSSKILCCLIRPPLALAELAIVSACVQIGLA
                     LPMAAWFHRVSWSGIAANVFVVPLISLIVPIGFLTVGTGWPLLAHLLSSLVTAMAGIV
                     GWHARWVWLEARVPDPPP"
BASE COUNT         1203 a         1936 c         1926 g         1282 t
ORIGIN      
        1 gagattgcgc cggatcgagt tccgaatggg aatcaatttt accggcagcc ttgtggaata
       61 cagcagttcg gcaaatcgcc ggcccgcctc gcctccacac accgccaggg cgagcaccgg
      121 agcattcagg ccctgaatca cttgcgcggc gttgattccc ttgccgccgg cctgctcgag
      181 cgaagacctg acatagatgc gatcctcata gctgatgcga tcggtggtga cggtgtgatc
      241 gatggcagga ttgaccgtca gcgtgagaat cataaaatcg gacgagcggc ttcctcgcgc
      301 aaagctttta actctgcttc gagttgcgcc acgcgttcgt gcaacccgtt aatcgcctgt
      361 tccaatgggt cgggcaggcg gccatgttcc aggatatcat cgtcctcgtc tttcacactc
      421 actccgtcaa tagccaccac gcgcccgggc acgcccacga cggtggaatg aggcggtact
      481 gatttcacga ccaccgatcc ggcgccgatc ttgctacgtt cgccgatggt gatgtttccc
      541 aaaatgctcg ctccggttcc tattactaca tgattgccga tggtcgggtg ccgcttgccc
      601 ttctccttac ccgtgccccc gagggtgacg ccttgataaa gcagaacgtc gtctccaatc
      661 tcggctgtct cgccaattac cgcgcccatg ccgtgatcaa tgaaaaaccg gcggccgatg
      721 cgagcaccgg gatgaatttc gatccccgtg aggagccggc tgaactggga aatcagccgc
      781 ggcagcagcg gcactcgcca cacgtaaagc cggtgggcca gccggtgcaa gagaatcgcg
      841 tgcaggcccg gatagcacaa cagcacttcg agggtgctct tggctgcggg atcttcttta
      901 aaaacagtgt cgatctgttc cttaatggcc cgaaacataa ggcctccttg ggggaaccgc
      961 gtaaccgatg gattaacaaa gtggaaaaag aatcagatgc gccgacgcga ggtcgggcca
     1021 ctacagcagc atttgagctg ttggaacata tttgaagatt agacaaattg gctctgattg
     1081 tcaaactaca cggccacaaa gcgcggaaga gcttaggaag cccggcctgg cgccacctct
     1141 ggcgatcggc tccagcgcag catggccagg gcccacgcgg tcgccggcaa caacatcgca
     1201 agaccggtga caagcgccgt agtgcgcaga gccataccgt gatccacagc aaagcctgtg
     1261 aggtaggccg acacggaagc ggaaagcatg ttcagcccga agtcggcggc gaacacacgc
     1321 ccgcgaaacc ggtcttcggt gttcaattgc aatagcgtcg tcgaaaaaac ccagattagc
     1381 gatccgccgg aatgagccgc gatcacgcat gccgccgccg ccgcgagtgt aagtgcgcgg
     1441 ctgaagagaa tatagaaagc gccgactgtg aggaaccccg ccagcagccc aatgcgcatc
     1501 cggcgctgat cggaaccggc gaagcgcccg ccgatgaagg gccccaccag cgctccgaag
     1561 ccgcgggaca tgaataaaac gctcatgccg agcacaccgc cggcgggatc accgaactcg
     1621 cgttcgccca tcagagggag caacacgagg ttggcgccga caaaccccag gccgaatttg
     1681 acgaaaagca aggacatcag cttcaagtcc ccccggacgt agcgtactcc ttccagaacg
     1741 gggccaagcc ccaggatatc cagccactga agacgctgat gggtttccag atgcggctcg
     1801 cgaaagcgca taccggcgag cagcgcggcc gaagcgagaa aagagagcga gttcagaacg
     1861 aatgcggctt cccggccaaa gaagcgcacc gccagaccgc caagacccgc gccgaccgcc
     1921 agattaaagg accaggtggc ggcggagagg gcattggccg caatcacctg acgctcttcg
     1981 acaatattgg gaattacagc gctgcggccg ggctcaaaga aggcagcgag aatcacttcc
     2041 gctatcagga gtacataaag aagccaaagc tgctcccgcg ttcgcaccgc gagcatcccg
     2101 agcacgatcc cggcgcgaag cacgtccgct gtgatcatca ccgccttgcg gctcacccgg
     2161 tcgttcactg ctccggcagt ggggccaaga aagaatgccg gcaggatctg agccagcacg
     2221 acagctccca ccagttgggc cgacccagtc aggcgcagca cgagatcgta cacagccacc
     2281 gaatagaacc agtcgcccag ctcgcttacg atctgcgcca gccacaaccg gcgaaagttg
     2341 ggattttcgc gagtcagcct gtagtaagct gcgagagaaa tatgcgggct ggattcgtct
     2401 gcttccggct ggctcatggc agggcctcag gtatagagac ccgtcttctt tttcaccagc
     2461 tcgagcgtgg aattagcgat cggcgtcacg cgttcggcgc ccttctggag tatgtctctg
     2521 aggtagccgg gctgggcgat aatttcacgg tatttgcgct gaatcggcgc gagactcgcg
     2581 gccactgcat cggctacttc cttcttcaaa ttggaatacc gcatgccgct gaagtgtttt
     2641 cgcatctcct cgtcgctcca gccagtaaaa ccttggaaga tcgcgagcag gttggccacg
     2701 ccggggctca aatcctcgaa atcaacagaa ggcttggcgt cggtggtcgc tcgcccgatt
     2761 accttctgaa tccgctccgg cgggtccaga agcgcgacgg cgtgcatcga tcccggcgcc
     2821 gatttgctca tcttgacggt gggatcgtcg aggcccatca ctcgcgcgcc gaccgaaggc
     2881 agcttggttt gaggcacgac gaatgtttcc ccgtaaatcg aattgaatcg cccggcgatg
     2941 tcgcgcgtga gttcgagatg ctgcgcctga tcctcgccta cgggaacgat ggcggcgtta
     3001 tacagcagga tgtcggctgc catgagcact ggatagagga acaggcccgc cccgacggac
     3061 tcttgctgct ccgccttggc cttaaactgc gtcatgcggc tcagccagcc cattggcgtc
     3121 acacaggtca ggatccaggc cagctccacg tgcgccggca ccgccgattg cacaaagatg
     3181 gcgcaatgtt ccggatcaat gccggcagcg atgtagatcg cggcgatctc ctcgattttt
     3241 tgccgcagtt cggccggatc ctggtagacc gtgatggcat gaagatccac gatgccgaag
     3301 atcgtctcgt agtcgtattg aattttggtc cagttcttca gcgcgcccag gtagttgccg
     3361 atatgcaggc tcccggacgg ctgcatcgcc gaaaacaggc gtggcttcat aactcaagcg
     3421 gagaatgaat gtttcattct acagtagcga taggcgttcc tgccggccgg ctgctggcgg
     3481 gatggccggg gaagctttgt gtcgatccca gtggaaatcg agaagctcgt ttacggcggc
     3541 cagggcctgg gccggttcga tggccgcgtg gttctggcgc ccttcgtgct gcccggagaa
     3601 agggtacggg tcgagccgga gaaagagtca tcccatctga tcaccgcccg gacgattgcc
     3661 gtcgagactc cggccgtctg gcggaccccc gcgccttgcc cttacttcgg gatctgcggc
     3721 ggctgccatt accagcacat gccttacgag cgccagctag aagcgaaaag agccattctc
     3781 ctcgagacgc tcgcgcgcct gggaaaaatt gaatggagcg gcccgctcga gatcatcgcc
     3841 gccgagccct gggcttaccg gaaccgtgtg cagcttcggg tgcggaaaca ggcgggccgg
     3901 tttcaaatcg gctacttcga attcggctcg caccggctgt gtccggtcga gcactgcccg
     3961 atcagctccc cgggaatcaa caaggcgctg gccgcactgg cccgcatagg caaggcgcgg
     4021 cgatttcccg attttgtgca ggctatcgag ctgttcacca acgagcgtga agtgcagctc
     4081 aatgtgctcg aatcgagccg tcccgtcgcg cgccgcttct ttgactggtg tgcggccgag
     4141 atcgaggggt tttgccgctc cgattttctg gattatccgg cggggcccga catttatcgc
     4201 gtcagcagcc ggtcattttt tcaggtaaac cggtttttgg cctcgcgcct ggctgagatt
     4261 gcggccggcg gtgagcgagg caatacggcc gtcgatctct atgccggggt cggcctgttc
     4321 agcctgagtc tggcccggca gttttctcga gctgtcgcca atgactcgaa ccgcagcgcc
     4381 tgcctggatc tcgtttacaa tgcggagcgc gctggcgtgg cgatagaggt acaacaggcg
     4441 ccggccgatc agttcctgga aggcttcacg gaaaagatag attttctgct ggcagatccg
     4501 ccgcgcgccg gcctggggaa cgccgtgaca gcgtcgatcg cccgcctgcg ccccaggcgc
     4561 ctgacgatcg tctcatgcga cccgtccact ctggcccgcg atttgatgcg cttattggcg
     4621 gccggattta aaataatttc aatgaaaatc gttgatttat ttccccagac gtttcatatt
     4681 gagactgtag tgacattggc cggataaaat atcagacgcc gtgtaccggc gggcagatcc
     4741 cgttttaatt ggcgtctggc agcgcgctat gcggtatccg ctggctgcgc cgatggcggc
     4801 cgttgcagcc ggaattcttg cggcgcagta cgcgaccttt tcttttcggg aactgtcaac
     4861 ttccattctc cttcttctgg ctttggctct ggccggattg ctgcggggag ccttgcgcgc
     4921 cggcgcggct gcttgcctgt tgggttttag cggtgtcggc gccctgctcg cttcgttacc
     4981 tcatcctgcg gaccgcaccc ggattgatct cgtagccgaa agagagcatc tggatttgcg
     5041 ggatcctatc cgccttgccg gatgggtgcg ggtgccgccc acagtccgtc gcgaccgcca
     5101 tcagtttgtc ctggcggtgg agtcgatcgg cgcgggaact gcggcttccg gcggcgttcg
     5161 cgtcacgatt ctgcgcagaa ccggcgaggc tgagcccgag ctccactatg gcgaccatct
     5221 tgagttcctg gcgcggctgc gccatatccg caattttaaa aatgcgggga gcttcgatcg
     5281 cgccgccgcc ttgctccgcc aggatattga gatgaccgcg tctgtgcgcg ccggcgtgcc
     5341 cctgagaaag atcgagggcc gtcgcgggcg gccgtttccc gcgcttatct ggagggcccg
     5401 cgagtgggtg gagcggcgct tggaccggct gctcggcccg gaatcgcgaa gcggaggcat
     5461 tgtcaaggca atgttgctgg gcgacgaggc ttacatcgac cccgcgttga gcctagcgtt
     5521 tcaacggacc ggcacatacc acgctctcgt cgttgccggg ttgcacgtgg gagctctagc
     5581 ctgccttttt ttgtttctat tccgcagcct gcggatttcc ggcggctggt cggccgtggc
     5641 tacggcggcg ccgctggcag gctttgtgct tttatgcggg tcgcggctgc ccacggttcg
     5701 cgccgcgtgc atggtgacag gctatttgat cgcccgcgca ttctatcgcc atcgccgcgc
     5761 gctgaatatt ctggcgggaa cggccctggg ctttctgata gtagacccgg cggacttgtt
     5821 cgacgtcagc ttccagcttt cctttctgtc tgtgggcgct atcgcggcga tagcggttcc
     5881 cgtcatcgag cgattcctcg atccgtaccg gctggctctc gtcgaccttc ctgaccggag
     5941 ccgcgatctc catctgccgc cgaaggtggc gcaaatacgt atcgaatggc gcgcgatcgc
     6001 agcccggctg ccctttgcac agcgaaattc ctcaaaaata ctgtgctgtc tgatccggcc
     6061 gccgctggcg ctcgcggaac tggccatcgt ttccgcctgc gtgcagatcg ggctggcctt
     6121 gccgatggcc gcatggttcc accgtgtctc atggagcggc attgcggcga atgtgttcgt
     6181 cgtgcctttg atcagtttga ttgtgccaat aggcttcctt accgtcggaa ccggctggcc
     6241 ccttctcgcg cacctgcttt cttcgctggt aacggccatg gccggcattg tcggctggca
     6301 cgcccgttgg gtttggctgg aagctcgcgt gccggacccg cccccct
//
LOCUS       DASREK010000054         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1514498, whole genome shotgun sequence.
ACCESSION   DASREK010000054 DASREK010000000
VERSION     DASREK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514498"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFA54_03255"
     CDS             <1..895
                     /locus_tag="VFA54_03255"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZS49851.1"
                     /translation="SKVSDRAKRRGGPQLGTLGSGNHFLEIQYVDKIHFPEAARVLGL
                     EEDQVVVLIHCGSRGLGHQVCTDFLADMNAAMRKYDIHVRDRQLACVPIQSPEGQAYL
                     GAMRAAANFAWANRQAIMHALRHSFLKVFGRDCRLNLVYDVCHNIAKQERYEIDGKKR
                     DVLVHRKGATRAYPPGHREIPEAYRAIGQPVLIPGSMGTASWVLVGTEGSMRETFGSV
                     CHGAGRLLSRTAAKRGRDARQVQKKLEEQGIVVKSETRDGILEEVPEAYKDVDEVIEV
                     VHQAGLARKVARLRPMGVIKG"
     gene            1014..1790
                     /locus_tag="VFA54_03260"
     CDS             1014..1790
                     /locus_tag="VFA54_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS49852.1"
                     /translation="MPDPNCALCDGTGWKPVERGEVSGVERCECNQSLRPAQLLQSAR
                     IPPRFEKASFENFRLPDRRENPVANEALSKSILDAKVFSREYPLTEKPGLMFMGLPGV
                     GKTHLAVAVLKVLLEKGFEGLFVDYQSLLEGILASYDRTVGSGAREAYQDALDAEILL
                     IDDLGARRLSEWAEDTVFSIINHRYNARKATIVTTNLPDEAVGDKLAEKERGSGQYRV
                     RDSLADRIGSRARSRLFEMCKLIRITATEDYRLRGVRAPQ"
     gene            1808..>2562
                     /locus_tag="VFA54_03265"
     CDS             1808..>2562
                     /locus_tag="VFA54_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49853.1"
                     /translation="MNATEAGAPRRKIAWKRPGGNKQRFAVRWKLRAASPALLLLSAG
                     VALAAIRQVQGRPVYFAGEKPFFVHSATFAYQQYPKDLWADLLVNLKSMGFNTVRLPV
                     PQPQNENASRDLPQVLRLARQLGFRIWFSGGGSAADPESAAAARGVSVLGGLDGGSVR
                     WFPAAGNAPPSKRLLTLADVIAVRRLLPRPPQMPVVAAFDAGWSSGGDVQARPSDSSN
                     YLLATRELLADGVKALNCSAVLEELSGGREAAV"
BASE COUNT          496 a          746 c          859 g          461 t
ORIGIN      
        1 aagcaaggtg tctgaccgtg cgaagcggcg cggcgggccg caactcggta cgctgggcag
       61 cggcaaccac tttctcgaaa ttcagtacgt cgataaaata cactttccgg aagccgcgcg
      121 agtcctggga ctcgaagaag accaagtcgt ggttttgatt cattgcgggt cccgcggact
      181 gggccaccag gtttgcaccg attttctggc cgatatgaat gcggccatgc gaaagtacga
      241 catccacgtg cgcgaccggc agcttgcttg tgtgccgatt cagtcgccgg agggacaggc
      301 ttatctcggg gccatgcggg ccgccgccaa cttcgcttgg gcgaaccgtc aggctatcat
      361 gcacgcgttg cgccattcgt tcctgaaagt ttttgggcgt gattgccgtc tcaatctggt
      421 ctacgacgtc tgccacaata tcgccaagca ggagcgctac gagatagacg gcaagaagcg
      481 cgacgtgctg gtgcatcgga agggcgcaac ccgcgcttac ccgccgggcc accgcgagat
      541 cccggaggcg taccgcgcga tcgggcagcc ggtgctcatc cctggtagca tgggcacagc
      601 gtcctgggtg ctggtgggga cggaaggctc gatgcgggag actttcggca gcgtctgtca
      661 cggagcgggc cggctgctca gccggacggc ggcgaaaagg ggacgcgacg cgcggcaagt
      721 ccaaaagaag ctggaagagc agggtattgt cgtcaaaagc gaaacccgtg atggcatcct
      781 cgaggaggtg cccgaagcgt ataaagacgt ggacgaagtg atcgaggtgg ttcaccaggc
      841 tggactggcc cgcaaggtgg ctcgcctgcg tccgatgggg gtcatcaagg gatgacgagc
      901 cacccggtcc aggagcaaga gaagcggagc cgcgacgagc gccaccgaga aaaacacgat
      961 gctgatgggc ggatggccgg gccggctggc ggctagggga gcggagtgca tgcatgcctg
     1021 atcccaattg cgcgctgtgc gacggcacgg gatggaaacc ggtggaacgc ggtgaggtct
     1081 ccggcgtaga gcgttgcgag tgcaaccaaa gcttgcgtcc cgcccagttg ctgcaatccg
     1141 cgcgcattcc gccccgtttc gaaaaagcgt ctttcgaaaa tttccgtctg ccggaccggc
     1201 gggaaaatcc ggtggccaac gaagcgctga gcaaaagcat actggacgcc aaggtcttct
     1261 cccgcgagta tccgctcacg gagaaaccgg ggctgatgtt tatgggacta cccggcgtgg
     1321 gcaagactca tctggctgtg gctgtcctga aggtgcttct cgaaaaaggc ttcgaaggat
     1381 tatttgtgga ctaccagagc ctgctcgaag gaatcctcgc cagctatgat cgaacagtcg
     1441 gctcgggcgc ccgagaagca tatcaggacg ccctggatgc cgagatcctg ctgattgacg
     1501 atctcggcgc gcgccgatta agcgaatggg cggaggatac ggtgttcagc attatcaatc
     1561 atcgttataa cgcccgaaaa gccaccattg tcaccaccaa tctgcccgat gaggctgtag
     1621 gcgacaagct tgccgagaaa gagcgaggtt cgggccaata ccgcgtgagg gattccctgg
     1681 ccgaccgcat cggctcccgc gcccgctcgc ggctgtttga aatgtgcaag ctgatccgca
     1741 tcacggcaac cgaagactac cgtcttcgag gggtgcgcgc gccgcagtaa cggaaaaaca
     1801 ggaagtgatg aacgcaacgg aagcgggagc gcctaggcgc aagatcgcgt ggaagcgccc
     1861 gggcggaaac aagcagaggt tcgcggttcg gtggaaactc cgggccgcct cgccggcgct
     1921 tctactgctt tcggctggcg tggcgctggc tgctattcgc caggtgcagg ggcgtcctgt
     1981 ttacttcgcc ggcgagaagc ccttcttcgt ccacagtgcg acgttcgcct atcagcaata
     2041 ccccaaggat ttgtgggcgg atctcctggt gaacctgaag agcatgggct tcaatacggt
     2101 ccggcttccg gttccgcagc cccagaacga gaatgccagc cgcgatctgc cgcaagtctt
     2161 gcggctggcg aggcaactcg gcttccgaat atggttctct ggcgggggct ccgcagcgga
     2221 tccggaaagc gccgcggccg ctcgcggcgt ttccgtcctg ggcggcctcg atggcggttc
     2281 ggtgcgctgg tttcccgctg ccgggaatgc gcctccttcc aagcgcctgc tcactttggc
     2341 cgatgtgatt gccgtacggc gtctgctgcc ccggccgccg cagatgcccg tcgtcgccgc
     2401 atttgacgcc ggctggagca gcggcggcga cgttcaggcc cgcccttccg attcttccaa
     2461 ttacctgctg gccacgcgcg agctgctggc ggacggcgtc aaggcgctga actgttcggc
     2521 ggtcctggag gagctgtcag gggggcggga agcggcggtg ag
//
LOCUS       DASREK010000055        13418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_362448, whole genome shotgun sequence.
ACCESSION   DASREK010000055 DASREK010000000
VERSION     DASREK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13418
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362448"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..507
                     /locus_tag="VFA54_03270"
     CDS             322..507
                     /locus_tag="VFA54_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZS49854.1"
                     /translation="MNKLFTLVAKIVREEHGQDLVEYALLAGFIAVAAGALLPGISTS
                     ISTIFSRMASVLSNAAA"
     gene            complement(688..1197)
                     /locus_tag="VFA54_03275"
     CDS             complement(688..1197)
                     /locus_tag="VFA54_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZS49855.1"
                     /translation="MKTIPGDHMVPKERRGQPRTRLRRHYPVTIARPGAESSYRASLI
                     DVSAGGCAVKTRAPIRPGRPVTMAGEVRSDNLRTRIHAKAVVAYSLRLLPGVYRLGLR
                     YKALRWGSAGETALGVIFQLLVHETDTAIAEDLAPSPVRARGPGAESRPDKGSRRNQV
                     RRQPKRTSS"
     gene            1580..2860
                     /locus_tag="VFA54_03280"
     CDS             1580..2860
                     /locus_tag="VFA54_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS49856.1"
                     /translation="MAASQVRTETPTRARLWVLCLSFLVSLITYLDRVCISAAAPAMS
                     QDLSLTSMQMGYVFSVFALSYAVFEIPAGWWGDRVGQRSVLTRIVSGWSIFTILTGLA
                     RSYPVLLITRFLFGGAEAGAYPTLSRALARWFPADERAWANGVMWTGARLGGAIAPPV
                     AAILIASIGWRATFGLFGCIGFAWCAVFWAWFRDDPAEHKGVNAAELEKIRGNAAPAP
                     SRAHFHAPWARIFSSGTVWALFGMYFCSAYGFWFFVSWLPTFLMREHGLSLAKSGVYS
                     SLPLASGAIACVAGGGLSDWLVRCTGNVKWSRRAIGAGGFILAAAGFGFASKVHSPLA
                     AVLLLALADGSHDLALPVAWATVTDVGGRSGGTTSAFMNMASSLSAMVSSVSAAWLAT
                     RFGSFDAMLAAASGVYLIGAILWLRIDPTQRVNA"
     gene            3018..3293
                     /locus_tag="VFA54_03285"
     CDS             3018..3293
                     /locus_tag="VFA54_03285"
                     /inference="COORDINATES: protein motif:HMM:NF041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HZS49857.1"
                     /translation="MTSKALRKGIRTGIAVTGLTISTLHLPALAQNTPPPANPPMTEN
                     APMNYGRGGDWGLWGLVGLLGLFGLRGGRRALYRTEPGETPRTTRAA"
     gene            3743..3934
                     /locus_tag="VFA54_03290"
     CDS             3743..3934
                     /locus_tag="VFA54_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZS49858.1"
                     /translation="MAAARIAIAPMKAAQVSKPGGDFEIVEREIRKPGAGQVRIKVQA
                     CGICHSDVLTKEGEWPGIQ"
     gene            3947..4156
                     /locus_tag="VFA54_03295"
                     /pseudo
     CDS             3947..4156
                     /locus_tag="VFA54_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            4256..4777
                     /locus_tag="VFA54_03300"
     CDS             4256..4777
                     /locus_tag="VFA54_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZS49859.1"
                     /translation="MPGDLVAVAGLGGLGHLGIQFANKFGYKVAAIGRGSENAELARK
                     LGASVYIDSKATNAAEALLKLGGACVILATAPSSKSMSELIGGLGPNGKLLVVGVAFD
                     PIEVTPVQLISGSRIIQGWAAGTPADSEDTLRFADLTGVRPMIETYPLEKAAEAYARM
                     MSGSAQFRVVLTM"
     gene            complement(4849..5814)
                     /locus_tag="VFA54_03305"
     CDS             complement(4849..5814)
                     /locus_tag="VFA54_03305"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZS49860.1"
                     /translation="MPLQELLVEELKDLLDAEKQLVRALPKLAKTASDAELAGLFRDH
                     LEQTKGHVGRLEQAFEILGARPKSKPCLGMKGLVEEGRDVAQEDLEGELLDSAILGAA
                     RKVEHYEMAGYENACSIARMLGNSQAEELLQQTLDEERTTDQKLADVSKRLLQQVMAE
                     QGAAPEEESETGGRRALRGAEKQARPQPVRRASSSRRSQPAARSRAKFRTSASSAAGR
                     SSGALSKITTDHEEIRRWAEERGGKPAAVKGTGGKGDIGMIRIDFPGYSGGESLQPIS
                     WDEWFQKFEDSNLALVYQERTATGQKSNFNKLVSREEVHPRKRAA"
     gene            complement(6337..7464)
                     /locus_tag="VFA54_03310"
     CDS             complement(6337..7464)
                     /locus_tag="VFA54_03310"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZS49861.1"
                     /translation="MRITDVEAVHLRLPNVEEIADGTQDVLVIRVHTDAGLTGIGEVS
                     SQSYVCKAIFEAPPSAERRHGLAHLLRGQDPLDIEGLWRRMYYHTNRYGRRGAVIHAI
                     SGADIALWDLKGKALGKTVSELLAPASSEKTRQRLIRAYASYLFGSTPSETASLAREA
                     VDLGLTAVKFGWGPFGKDLAGDMAHVEAARRAIGDQRDLMVDAGCAFDVPSALAWAGR
                     LASFQIGWLEEPLSQDDLQGYADLCARSPVPIACGEGEVTRWGFEELIERGVHVIQPD
                     VAICGGLTVAVQASALADAAGRRCVPHCFSTGINLAASLHWMAACEHGDLVEYCLRPS
                     PLLRKLVRNLPPLVDGRVRAPQGPGLGIELDEQIVEQFRVR"
     gene            complement(7528..8337)
                     /locus_tag="VFA54_03315"
     CDS             complement(7528..8337)
                     /locus_tag="VFA54_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZS49862.1"
                     /translation="MQGPKLKYRYDHYTWPEMKEVIARQPVCVLPIGSVEDHGRHLPL
                     DTDNFLIGAICDEVARRAPEEVLLLPPIPYGFEDHHMSFPGTITVRPEHLEAFVLDIT
                     LSLAHHGFRKILLADGHGSNMPILDLAARKTIIQSDALCACFIWPSLIADLLRKTRES
                     EFPGGMSHACELETSVYLYLNESAVQMERAEKEIGFHKSKYYWHDLSGGPAVRMVEWW
                     TRISNSGVIGDPTVATSEKGRLWYEATVANLVEFIHEFRAFEQRPKIDLHM"
     gene            8463..8783
                     /locus_tag="VFA54_03320"
     CDS             8463..8783
                     /locus_tag="VFA54_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49863.1"
                     /translation="MKRRVYRTMYERIAVFLFSLLMALGSLAGAVWVIATGQAVNMDG
                     IFMLLAFLVFALIFALCIWLLIKGALASPAPAAAPAKPAAERAAPAPAVKAAQTQSQP
                     AVTR"
     gene            complement(8791..10095)
                     /locus_tag="VFA54_03325"
     CDS             complement(8791..10095)
                     /locus_tag="VFA54_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZS49864.1"
                     /translation="MPENQPKIGREPPAQQDGRRLLLLSTTTGYQAQAFRDAARKIGA
                     SLVLASDRCHVLEDPWHDGAIPVRFENPEESAQKILEFARANPIQGLTAIGDPPTLTA
                     ALVARELSLSYHPVEAVETCRDKFKMRERCRAAGLPLPWYRRWRANEDPAAIAGAVPF
                     PCVLKPLGLSASRGVIRADSAAEFQAAFRRIAALLRSPEIRRRREESAEWIQVESYIP
                     GCEVAVEGILTHGRLRVLAVFDKPDPLEGPYFEETIYVTPSRLTGATLEAIHSSLQAA
                     VDAVELRHGPLHAELRVNDGGAWVLEMAARPIGGLCARALRFGGGVGLEELILRHALG
                     EPVEDLPREKASSGVMMIPIPREGFLEKVENVPEAEAVPGIEDVEITAKERQKLIPLP
                     EGASYLGFLFARAESSELVEAALREAHGKLRFVISPSLPVVR"
     gene            10444..10896
                     /locus_tag="VFA54_03330"
     CDS             10444..10896
                     /locus_tag="VFA54_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HZS49865.1"
                     /translation="MDAEVYPDSRVASYITQNFIPVKIHIKEQHAAFDRFGVQWTPTL
                     LILDPQGRERYRFEGYLPPEDFLAQLHLGLARVAFANQQWNEAERRYREIVEQYPKTE
                     TAAEALYWAGVVRYKASGDAAALKETAHQFQSRCTSSAWAKKASVWAA"
     gene            complement(10949..11752)
                     /locus_tag="VFA54_03335"
     CDS             complement(10949..11752)
                     /locus_tag="VFA54_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS49866.1"
                     /translation="MESNHLEKVVLRRVGEAIGKFKMIRDGDRVAVAVSGGKDSLTLL
                     DALLVLQKRSPAKFTVCAFTVEQGKFLRPIAPLGDYMKAHEISWTCFHDAPSFRLIEE
                     QPGHGCDLCSRYRRRAVYEIARGLGANVIAFGHTADDFCEALLRNTLFTGRISALPPV
                     TYSRERDFRIIRPLLYVTEKITQAYAGQKQFPVIPCGCSQRTGEVRRSLRGFMDAWKK
                     DHPHVLENMLSAMGRLDTNRLLDTRFLSLDGGAIESGEPDLLPIRPAAV"
     gene            complement(11961..>13418)
                     /locus_tag="VFA54_03340"
     CDS             complement(11961..>13418)
                     /locus_tag="VFA54_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS49867.1"
                     /translation="LRLQIDSMPVEIDQRDRRMVQLQIEQQALAKETDPHSRERRRII
                     ENGLERLRAETAGLKERWAREKAVIQRVRQLKEELESLKQEEEKATREGDLGKAAELH
                     YGRMVEVRKELEQASAELEAEQKATPLLRGEIDEEDIARIVAKWTGIPVSRMLEGERA
                     KLVHMEDRLRRRVVGQEEALVRLSNAIRRNRAGLSDPKRPIGSFIFLGPTGVGKTELG
                     RALAEFLFDDEKAVVRIDMTEYMEKHAVSRMIGAPPGYVGYEEGGQLTEHVRRRPYSV
                     VLFDEIEKAHPEVFNALLQILDDGRLTDGKGRTVDFKNTVIIMTSNVGSGAIKEMGPI
                     GFSLTHDTQKKNDDARKKLLSALQQTFRPEFLNRIDDIIVFNRLGKAEIEQIIDIQLR
                     VLRAQLAEQRITLELTPQARELIIAEGYDPNYGARPMKRAIQRLVQDPLAFKLLNGDF
                     QPGDAILAHAVEGRPELRFEKMHTPAEMEEEAVAV"
BASE COUNT         2496 a         4181 c         4059 g         2682 t
ORIGIN      
        1 gaaaaggaga tgggcgtggc attcgaggag cgcgggctgg cctagaaaca caaggacttg
       61 ctcctggaac tctcacagcc ggcgcgcccg tgcgggatgc gttcacggcg tccagccatc
      121 ctttttgcct tccggacccg gccttagctc cacggattga ctgaggactt ctcgggttcg
      181 ccccctcgga actttagggc gtagaaccta ggcaaaaaca gtactaacgc tcaattgccc
      241 ggacccgcga tgctgccgca gaatgatccc tgcaaaccgg cgaaccacag agccgcgaat
      301 tcaaaaaaca tggagagaga aatgaacaaa cttttcactt tggtagcgaa gatcgtccgc
      361 gaagagcacg gccaggatct cgtggaatac gcgctgctgg cgggcttcat cgctgtcgct
      421 gccggcgccc tgttgcccgg tatctcgacc agcatcagca cgatcttcag ccgcatggct
      481 tccgttctgt ccaacgccgc tgcgtaatcg ggccgtggca ttccgcaagg cggaggcgct
      541 ccatcccgtt tgcctccgcc ttgccccatt tcagattcgg ccactcggcc gaatctaccg
      601 agcagcctca gtcaccctgc cgaacaccgc gtttgattgc ccgcctctag tttcaccgga
      661 gggcgcggcc cggtgaaacg cccgctctca ggatgacgtc cgtttcggct gcctccgaac
      721 ctggtttctg cgggaacctt tgtctggccg gctttcagcc cccggccccc gagctcgaac
      781 cggcgaaggc gcaagatcct ctgcaatcgc cgtgtccgtc tcgtggacca gcagttgaaa
      841 gatgacgccc aaggctgtct cgccggcgct gccccaccgg agcgccttgt agcgcaagcc
      901 gagccgatat acgcccggca ggaggcgcag gctgtaggcg accaccgctt ttgcatggat
      961 tcgggtgcgc aagttgtcgc ttcgtacttc cccggccatt gtcaccgggc ggcctggccg
     1021 gatcggcgcg cgcgtcttta ctgcgcatcc gccggcgctg acgtcgatga ggctcgcccg
     1081 gtaagacgac tcggccccgg ggcgggcaat tgtaaccggg tagtgccgac gaagccgggt
     1141 gcggggctgc ccgcggcgtt ctttcggaac catatgatcg ccaggaatcg ttttcacttt
     1201 cggactaact cgcctctgcg ccctcggaaa tacctatcct caagtggagc cgagccggca
     1261 gcaacttgtt agccaaaaca caagtatcga gatagcaggc ctataggtgc tcgggaggcc
     1321 gtgaacaccc cgtcaatttc tgcccagcgg gccgaaattt acacgtctcc ggcgttggta
     1381 aactgcgcat ttctcctcaa aatacgcttt cattgtctgc tcagcattgc tcgcgcgttt
     1441 ccccggccgg gcttgacaga ggccgggttc gcaagctacg ttcagacgcc tcagcatgcc
     1501 ggaagaaaat acgaccggcc tcttcggacg tgtgggcata ccggcttcag agtgcaggag
     1561 cgggtgctga actaaattgg tggccgccag tcaggtcaga accgaaactc ccacccgggc
     1621 ccgcctatgg gtcttatgct taagctttct cgtttctctc attacctacc tcgatcgggt
     1681 ctgcatttca gcggcagcgc ctgcgatgtc ccaggacttg agccttacga gcatgcagat
     1741 ggggtacgtc ttcagcgtgt ttgctttgtc ctacgcggta ttcgaaatac ccgccggctg
     1801 gtggggtgac cgtgtcggac agcggagcgt tctcacgcgc atcgtctcgg gctggtccat
     1861 cttcacgatt cttaccggac tggcccgcag ttatccggta ctcctgatca ctcggtttct
     1921 tttcggcggc gccgaggcgg gcgcttaccc cacgctttcg cgggccttgg cgcggtggtt
     1981 tcccgccgat gagcgggcgt gggcgaacgg cgtgatgtgg accggagccc ggcttggggg
     2041 tgcgatcgcc cctccggttg cggcgatctt gattgcctct atcgggtggc gggcgacttt
     2101 cggtctcttc ggttgcatcg gatttgcatg gtgcgcggtc ttctgggcct ggttccgtga
     2161 tgatccggcg gagcataagg gggtgaacgc ggcagaactg gagaaaattc ggggaaatgc
     2221 cgcgccagcg ccgtcgcggg cgcactttca cgcgccgtgg gcgcgcattt tctccagcgg
     2281 caccgtctgg gccctgttcg ggatgtattt ctgctccgcc tacggctttt ggttctttgt
     2341 gagctggctg ccgaccttcc tcatgcgcga gcatgggctt tccttggcca agtcgggcgt
     2401 gtactcctcg ctgccgctcg cttccggggc catcgcttgc gtggcgggcg gaggcttatc
     2461 ggactggctc gtccgctgca ccggcaacgt caagtggtcg cggcgcgcaa tcggcgccgg
     2521 cggtttcatc ctggcagccg ccggcttcgg gttcgcgagc aaagttcata gcccgctcgc
     2581 tgcggtactc ttgctggctc tggccgacgg ctcgcatgac ctggccctgc ccgtcgcctg
     2641 ggccacggtg acggacgtgg gaggccgttc cggcggcaca accagtgcgt tcatgaacat
     2701 ggcttccagc ttgtccgcca tggtatcttc cgtttccgcc gcttggctgg cgacccggtt
     2761 tggatcgttt gatgccatgc tggcggccgc ttccggcgtt tatctcatcg gagcaatttt
     2821 gtggctccgt attgacccaa cccagcgggt gaacgcttga ttgcgaattc gcgccctgcc
     2881 cgggctttgc tacatggggc tccactgttc atggaagccc tctcccggcg cgccgcacgg
     2941 ccccatgcct gctcctctcg tgacgatggc gtcattggca gaatgcttgc accagcccgc
     3001 attcatggag gtttgtgatg acatccaagg cactcaggaa aggtatccgg acaggcatcg
     3061 ccgtgaccgg ccttacgatt agcaccttgc accttccggc gcttgctcag aacaccccgc
     3121 cgccggccaa cccgccgatg accgaaaacg caccgatgaa ctatggccgg ggcggcgatt
     3181 gggggttatg gggactcgta gggcttctgg gtttgttcgg gctccgcggc ggccgccgcg
     3241 ccctttaccg tacggaaccg ggagaaacac cacgcacaac cagagccgct tagagagaac
     3301 ggatagcctc ctctgatccc aactacgaag gcattcgttt caagttccag gcaaggaacc
     3361 ggagctacat cggcatcacg tagccggcac gcgagcgcca gcacaaaagc cgggtagctg
     3421 ctgcacagca gcatttgggg atcgctggac agggcgatgc gaaccgtccg gggttggcaa
     3481 ctccggagga tacccgtgat tagccggacg tggctgaccc cggttcggag tcgctgcaag
     3541 ctctgttcgg aagagcaacg aacctcagcc ggttggccgg tggcgtcgcg gggtgcaacc
     3601 cggcactccg ttcgagggtc gaggcatttt cgagctggcc ggtaaaagag gcttggcggc
     3661 ggacgcccgt atcgctccac ccaatgtgcc tcctgacaga ggtaataaca gttgtgaacg
     3721 tgagaactca ctaggaggca atatggcagc ggcgagaatc gcaatcgcgc caatgaaagc
     3781 ggcgcaggtt tcgaaaccgg gaggagattt cgagatagtc gagcgcgaga ttcgcaagcc
     3841 gggcgcgggg caggtgcgca tcaaagtgca ggcctgcggg atttgccaca gtgacgtgct
     3901 cacaaaagaa ggcgagtggc caggaattca gtagccccgt gttccaggac acgaagtcgc
     3961 aggcatcatt gatgaactag gcgcaggtgt ttccgaatgg aacaacggaa agcgcgtcgg
     4021 cgtcggatgg catggcggcc acgatggcac gtgtaccgcc tgccggcgcg gagattttcg
     4081 caattgccag aacctaaaaa ttcccggcat cagttatgac ggtggatacc agcaatatat
     4141 gctggctccc gtcgaggcgc tggtggaaat accccaaagt ctgggccccg ccgaagcagc
     4201 gccgctgctc tgcgccggaa ttacgacctt caatgcgctg cgccacagcg gcgccatgcc
     4261 cggtgatctc gttgcggtgg cgggtctggg tggtctgggc cacctcggaa ttcaattcgc
     4321 gaacaagttc ggttacaagg tcgcggctat cggacgcggg tcggaaaatg cggagctcgc
     4381 aaggaagctc ggggccagcg tctacatcga cagcaaagcg acaaacgcag cagaagcatt
     4441 gctgaaattg ggaggcgcct gcgtaattct ggcgacggcc ccgagttcca agtcgatgtc
     4501 cgagctgatc gggggcctgg ggccgaacgg caagctcctg gtggtcggcg tggcatttga
     4561 ccccattgaa gtaacgccgg tacagctcat ttctggaagc cgcatcattc agggctgggc
     4621 tgccggaacg ccagccgatt ccgaggatac acttcggttc gctgatttga ccggcgtccg
     4681 tccgatgatt gaaacctatc cgctcgaaaa agcggccgaa gcttacgcgc gaatgatgag
     4741 cggcagcgca cagttccgcg ttgtgctgac gatgtgaacc aacctggcga caacgccatt
     4801 agctggaggc aagagatccg ggttccaact cttcttctgc cggctgcgtc aggcagctct
     4861 tttccgggga tgcacctctt cgcggctgac aagtttgttg aaattgctct tctgccccgt
     4921 ggcggttctt tcctgataaa ccagggccag gttgctgtcc tcaaatttct ggaaccactc
     4981 gtcccacgag attggctgga gcgattcgcc cccgctgtaa ccagggaagt cgatccggat
     5041 catgccgatg tcgcccttcc cgccagtgcc cttgacagct gcgggcttcc cgccgcgttc
     5101 ttcagcccag cggcggattt cctcgtgatc cgtagtgatc ttcgatagcg cgccgctcga
     5161 gcggcccgcc gcggaagagg cgctcgtgcg gaatttggcc cggctgcgcg ccgccggctg
     5221 agaccttcgg gaactgctgg cgcgccgcac cggctgaggc ctcgcttgct tctctgcgcc
     5281 cctgagggcg cggcggccgc cggtctcgct ttcttcttcc ggcgcggctc cctgctcggc
     5341 catgacctgc tggagaagcc gcttggagac gtcagccagc ttctgatccg tcgtcctctc
     5401 ttcgtcgagc gtttgttgca gcagctcttc agcctggctg ttcccgagca tacgggctat
     5461 cgagcaggca ttctcatagc cggccatctc atagtgttcc actttgcggg cagcgcccag
     5521 aatggcagaa tcgagcagct caccttcgag atcttcctgc gcgacatcgc ggccctcttc
     5581 caccagcccc ttcattccga ggcacggttt gcttttcggc cgggcgccca agatttcgaa
     5641 ggcctgctcc agccggccga cgtgtccctt cgtctgttcc agatggtcgc gaaacaggcc
     5701 ggcgagctcg gcatcgctgg cggttttggc gagcttcggc agagcgcgga ccaactgctt
     5761 ctcggcatcg agaagatctt tcagttcttc aaccaacaat tcttgtagtg gcacgatggc
     5821 ctccccagag cgctccccct gcggggcgca cgcagaacgc ttcgcaattt cgaggccagc
     5881 acatcggccc ggccctgcaa caggaacctc agccttccct gaggccgcac tgccgtgacg
     5941 catgttactg ctggcgcgct cctgccggcc caagcttcgt tcgcgctccg gagagcgcag
     6001 tgttgcggag cagcggcgaa gaaaaattcc gcaatctgat ccggacgcca agctgaaaac
     6061 cgccacggcg atcataacgc tacgggtggt cattccccgt gatgggtaca tcggggcaga
     6121 ttttacatgc cgctttttcc gagattcgct ggtgcgatgc gcccccgccg gcgggcctca
     6181 gtacgataga aaacacgcgc accgggcact tggaataggc ccggcctatc ggatggatct
     6241 ggagaaatcg gcaggaccac ggcgaacccg tccgatctca aagccgccga aaaggacgag
     6301 accggccctc gtcggatccg gcgcatcgtt ttcccttcac cgcacccgga actgttcgac
     6361 aatttgttcg tcgagctcga ttcccaagcc cgggccttgg ggcgcccgga cccggccatc
     6421 gaccaggggc ggaagattgc gcaccagttt ccgaagcagc ggggaaggcc tgaggcaata
     6481 ttcgaccaga tcgccgtgct cgcaagccgc catccagtgc aaggaagccg cgaggttgat
     6541 gccggtcgaa aagcagtgcg gcacgcacct tcggccggcg gcatccgcca gggccgacgc
     6601 ctgcaccgcc acggtgaggc cgccgcaaat agccacgtcg ggctgaatca catggacccc
     6661 acgctcgatt agctcctcga aaccccatcg cgtgacttca ccctctccgc aggcgatggg
     6721 aaccggcgag cgagcgcaca agtccgcata accttgcaga tcgtcctgcg agagtggttc
     6781 ttcaagccag ccgatctgaa atgaagcgag acggccggcc catgcgaggg ccgagggcac
     6841 gtcgaaagcg catccggcgt ccaccatcaa gtcgcgttga tcgccgatcg cgcgcctcgc
     6901 ggcctcgacg tgagccatgt cacccgccag atctttgccg aacggccccc acccgaactt
     6961 cactgcggtg agtccgaggt ctacggcttc ccgcgcgaga ctggcggtct cgctgggagt
     7021 cgagccaaaa agatagctgg cgtacgcccg gatgaggcgc tggcgggtct tttcggaaga
     7081 cgccggcgca agcagctcgc tcactgtctt gccgagagct ttccctttga gatcccaaag
     7141 cgcgatgtcg gccccgctga tcgcgtgaat caccgcgccg cggcggccgt atcgattggt
     7201 gtggtaatac atccggcgcc aaaggccttc gatatcgagc gggtcctggc cgcgcagcag
     7261 gtgcgccaag ccgtgccgcc tttccgcgga agggggcgcc tcaaaaatcg ctttgcaaac
     7321 ataggactgg ctgctgactt cgccgatgcc cgtcaggccg gcatccgtgt ggacgcggat
     7381 caccagcacg tcctgcgtgc catcggcgat ctcttccacg ttcggcagcc gcaggtgcac
     7441 agcttctacg tccgtgatgc gcaagccggc tccttgggtg tgcggcgctg ttgtgcatcc
     7501 ggtcttgcgc cgcaaacggc tttctgctca catgtgcagg tcgatcttcg ggcgctgctc
     7561 gaaggcgcga aactcgtgga taaactcaac gaggttcgca actgtggctt cataccagag
     7621 cctgcctttc tcgctggtag ctacggtcgg gtcgccgatg acgccgctat tcgagatgcg
     7681 cgtccaccac tcgaccatcc ggacggccgg gccgccgctt aaatcgtgcc aatagtattt
     7741 cgacttatga aaaccgattt ccttctcggc tctctccatc tgcactgccg attcgttcag
     7801 atacaagtag acagaggttt ccaattcaca ggcgtgggac atgccgccag gaaattcact
     7861 ttcgcgcgtt tttctgagca gatcggcaat cagcgacggc cagatgaaac aggcgcagag
     7921 cgcatccgac tgaatgatcg tcttgcgggc ggccaaatcc agaatgggca tgttgctgcc
     7981 gtggccgtcg gcgagcagaa ttttgcggaa gccatgatgg gccagactca aagtgatgtc
     8041 gaggacgaaa gcttcgagat gttccgggcg cacggtaatt gttccgggga agctcatgtg
     8101 atgatcctca aacccgtaag gaatcggcgg cagcagcaag acctcctccg gggcgcgccg
     8161 cgccacttcg tcgcagatcg ctccgattag gaaattatcg gtgtccagag gcaggtgcct
     8221 gccatgatcc tcgaccgagc cgatcggcag cacgcatacc ggctgccgtg cgatcacctc
     8281 tttcatttcc ggccaggtgt aatgatcgta gcggtatttg agtttcggtc cctgcatgac
     8341 cagccaatgt agcacgcgcg gccggcggcc gaacaggcgg aaaagccgcc ggaaggaaag
     8401 ccgaatccac gtttttagcg gatcatgaaa cgcccaagcg ccgtgaatcg tagaataata
     8461 atatgaaacg gagggtgtac cggaccatgt acgagcggat agcagttttt ctcttcagcc
     8521 tgttgatggc gctaggttcg ctcgccggag ccgtgtgggt cattgctacg ggccaggccg
     8581 taaatatgga cggcattttt atgcttcttg cattcctggt atttgccttg attttcgcac
     8641 tttgtatttg gttattaatc aagggcgctc tggcgtcgcc ggccccggcc gctgcgccag
     8701 ccaagccggc ggctgagcgt gcggctccgg cgccggcggt gaaagcggct caaacacaat
     8761 cgcagcccgc cgtgacacgc tgaagaaagg ctaacggacc acaggcaagc tgggcgaaat
     8821 tacaaatcgc agtttcccgt gggcctcgcg caaagcggcc tccaccagtt cgctggactc
     8881 ggcgcgggca aagaggaagc caagataact cgccccttcg gggaggggaa tcaatttttg
     8941 cctttcttta gcggtgatct cgacgtcttc aatcccggga accgcttccg cttctggaac
     9001 attttcgact ttttccagaa atccctcccg gggaataggg atcatcatca cgccggaaga
     9061 ggctttttcc cgcggcaaat cctcgacggg ctcgccgaga gcatggcgga gaatcagttc
     9121 ttccaaaccg accccgccgc caaagcgcaa agcgcgagcg cagagccccc cgatcggccg
     9181 ggccgccatt tcgagcaccc aggccccgcc gtcattgact cgtaactcgg cgtgcaaagg
     9241 accgtgccgg agttcgaccg catccaccgc ggcttgcagg ctcgaatgga tggcctccag
     9301 cgtggccccg gtcagccgcg aaggagtgac gtaaatggtc tcctcgaaat acggcccttc
     9361 caggggatcg ggcttgtcaa acaccgccag cacccgcaac cggccgtgtg tgagaatgcc
     9421 ctccacggct acctcgcagc ccgggatata gctttcgacc tgaatccatt ccgccgattc
     9481 ctcgcgcctc ctgcggattt ccggcgaccg gagcagcgcc gcgatgcgcc ggaaggcagc
     9541 ctgaaactct gcggcactgt cagcccgaat cacgccgcgg ctcgccgaga gacccaaagg
     9601 cttcagtaca cacgggaagg gaacggcgcc ggcgatcgcc gccggatctt cgttcgcccg
     9661 ccagcggcga taccagggaa gcggcagccc tgcggcccgg cagcgctccc gcatcttgaa
     9721 tttatcccgg caggtctcga ccgcttcgac gggatgatag gagagactca gttcacgggc
     9781 aacgagggcg gctgtcaatg tcggggggtc cccgatagca gtcaaaccct ggatggggtt
     9841 tgcacgcgca aactccagta tcttctgcgc ggactcttcc gggttctcaa aacggactgg
     9901 tatcgcccca tcgtgccagg gatcttccag cacatggcag cggtcgctgg cgagaacaag
     9961 cgaagcgccg attttgcgcg cggcatcccg aaaagcttga gcctggtagc ccgtggtggt
    10021 cgagagcagc agcaagcgcc ggccgtcttg ctgcgccggc ggctcacggc caattttcgg
    10081 ctggttctct ggcatggaag aacttgcatc tactatcatc cctcagcccc cggacctgtg
    10141 gcggctgggt cctccaaaga caacacgccg gccggcaaac ccactcgaac ttcacgccgg
    10201 ttctgttgtt ggctgcttga aattcccggt ttggttagcc tcaaaacaag aagcgatttg
    10261 catttcgtaa gcatccttaa tttcgcaagc atccttaatg gacagatcca ttctttagga
    10321 ggtttcttat acccgttgaa tggacaaaag actatcgatg cagcgctggc cgcggcccgg
    10381 accggcaaca gaaacgttct catggacttc aatgccgctc cgatgtgagg ggcatgcgct
    10441 cggctggacg ccgaggtata tcccgatagc cgggtcgcaa gctacataac gcaaaatttt
    10501 atcccggtta agatccacat caaggagcag catgccgcgt tcgaccgctt tggagtccag
    10561 tggactccga ctctgctgat attggatccc cagggcaggg aacggtatcg cttcgagggt
    10621 tatttgccgc ccgaggactt tctcgcccag cttcatctgg gcctggcccg cgtggccttt
    10681 gccaaccagc agtggaatga ggccgagcgc cggtacaggg aaatcgtgga gcaatatccc
    10741 aaaacggaaa ccgctgcgga agcgctctac tgggcgggag tggtgcggta taaggccagc
    10801 ggcgacgctg ctgcgctgaa ggaaacggca catcaattcc aatcgcgctg taccagcagc
    10861 gcctgggcaa aaaaagcttc cgtgtgggcg gcctgacctg cggtcagatc cggatgtggc
    10921 ggaccggcct ggctccatgg agcggcgatc acaccgctgc ggggcgaatc ggcagcaggt
    10981 cgggttcacc gctttcgatg gcgccgccgt ccagagagag aaagcgggta tccagaagac
    11041 ggtttgtatc cagccggccc atggctgaga gcatattctc cagcacgtgc gggtggtcct
    11101 ttttccaggc gtccataaag ccccgcagcg agcggcgcac ttcaccggtc cgctgtgagc
    11161 agccgcacgg aatcaccgga aactgctttt gcccggcgta ggcctgggtg atcttttcgg
    11221 tcacataaag caggggccgg ataatccgga agtcgcgttc ccgggagtaa gtcaccggcg
    11281 gcagcgcgct gatgcgtccc gtaaacagcg tgttgcgcaa cagcgcttcg cagaagtcgt
    11341 cggccgtgtg gccgaacgct atgacgttgg cgcccagacc tcttgcaatt tcatataccg
    11401 cgcggcggcg gtaccggctg cacaggtcgc agccgtggcc gggctgttcc tcgatcaggc
    11461 ggaaggaagg ggcgtcgtga aagcaggtcc aggaaatctc gtgggccttc atataatcgc
    11521 ccagcggagc gattggccgg agaaatttgc cttgctcgac ggtaaacgcg catacggtga
    11581 acttggcggg cgagcgcttc tgcaagacta ggagcgcgtc cagtagggtg agagaatctt
    11641 tgcccccgga aacggcgacg gcgacgcggt cgccgtcgcg aatcattttg aattttccga
    11701 tcgcctcccc gacccggcgg agcacgacct tctcgagatg attggattcc ataagcctcg
    11761 ggccttcccg ccggccgtcc atcagttgcg ataagcgaga accgccgcaa gcttattata
    11821 tcttccagga atcccccgaa aagctccggc ccggatcggc agtttctctg acccacaaac
    11881 tacggccaat gcgaaaggga acctgagggc gcaggctcat cgtgcggcca taaagcgtgg
    11941 cggagaagag aggtggacgg tcagactgct accgcttctt cctccatctc cgcgggggta
    12001 tgcattttct cgaaccgcaa ttccggacgg ccttccaccg cgtgagcgag gatcgcgtcg
    12061 cccggctgga aatctccgtt gagcagcttg aacgccagcg gatcctgaac cagccgctga
    12121 atcgcccgtt tcatcggccg cgccccgtaa ttcgggtcgt acccctcggc gatgatcaat
    12181 tcgcgggctt gcggcgtcag ctccagtgta atccgctgct ccgccagttg ggcgcggagg
    12241 acgcgaagct ggatgtcgat aatctgctcg atttcggctt tgccaagccg gttgaacacg
    12301 atgatatcat cgatgcggtt cagaaactcg ggccggaatg tctgctgcaa cgcgctgagc
    12361 agcttcttgc gggcgtcatc gtttttcttt tgcgtgtcgt gcgtgagcga aaaaccgatg
    12421 ggtcccattt ccttgatcgc gccggagccg acgttggagg tcatgataat caccgtgttt
    12481 ttgaagtcca cggtccggcc tttgccgtcg gtcaggcggc cgtcatcgag gatttgcagc
    12541 agcgcattga acacttcggg atgcgccttc tcgatctcat cgaagagcac cacggaatac
    12601 ggccggcgcc ggacatgttc ggtaagctgg cctccttcct cgtaacccac gtatcccggc
    12661 ggcgcgccga tcatgcgtga gaccgcgtgc ttctccatgt attcggtcat gtcgatgcgc
    12721 accacggcct tttcgtcgtc gaagaggaat tcggcaagcg cccgcccgag ctcggttttg
    12781 ccgacgcccg tcggcccaag gaaaataaag ctgccgatcg ggcgcttcgg gtcgcttaag
    12841 ccggcgcggt tgcgccggat ggcattcgag agccgaacca gagcctcctc ctgacccact
    12901 acgcgccggc gaagccggtc ttccatatgg accagcttgg ctcgctcgcc ctccagcatc
    12961 cgcgagacgg ggatgcccgt ccacttggcg acgatccggg cgatatcttc ttcgtcaatc
    13021 tccccgcgca aaaggggcgt ggccttctgc tcggcctcca actccgcgga ggcttgctcc
    13081 agctccttcc gcacttccac catccggccg taatgcagct cggcggcttt gcccaaatcg
    13141 ccctcgcgcg tcgctttctc ttcttcctgc ttcaggctct ccagttcctc tttcaactgc
    13201 cgcacacgct ggatcacggc cttctcgcgc gcccagcgtt ccttcaggcc cgccgtttcg
    13261 gcgcgcaagc gttccagccc gttctcgata atgcgccgcc gttcgcggga atgcggatcc
    13321 gtctccttag ccaaagcctg ctgctcgatt tgcaactgca ccatgcggcg gtcgcgctgg
    13381 tcgatctcca ccggcatgga atcgatctgg agacggag
//
LOCUS       DASREK010000056        13688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1680853, whole genome shotgun sequence.
ACCESSION   DASREK010000056 DASREK010000000
VERSION     DASREK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13688
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680853"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFA54_03345"
     CDS             complement(<1..182)
                     /locus_tag="VFA54_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49868.1"
                     /translation="MIWRRAAVNAKKVAAKGEARRQRALAVATARRSPGTVSRLRLLR
                     GMIAAVMIFRPAACLVR"
     gene            416..3244
                     /locus_tag="VFA54_03350"
     CDS             416..3244
                     /locus_tag="VFA54_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS49869.1"
                     /translation="MLHRIRATRIAALGFMLLFAAAARAQETINYASVSGRVTDPSGA
                     VVSGARVTARQIDTNQISTTETDREGRFRLPYLKVGQYEITIQQPGFAEAVRPLRLTV
                     GAAFELPISLAVESPATNITVSGEGAVLEAARSQIAGTVSQTEVSSLPLNGRNFLDLA
                     LLVPGVSPTNTGSTQLFAETSAVPGQGISVSSQRNFSNSFIVDGLSANDDAAGLSGIF
                     YGLDVVREFQVVTSGGQAEFGRALGGYINMVTKSGTNTTHGDLYGYFRNQRFNAANAL
                     SHNKLPLTQAQYGASLGGPIARNRTFYFANFEQRQLNQSGIVTIAPANASAINARLAE
                     AGYQGPPIATGLYPNPVRTTNFLAKADHEVHSNDQFSVRYSLYDVNSNNSRGVGGLSA
                     VSAAAGLNDFDQTVAASNIITLSPRTVNETRGQFTYSGLQAPPNDAVGPSVSISGVAS
                     FGRLSSSPTGRLNRLYEITDNVSHQAGAHALRAGANFLYNDLKITFPRADRGSYSFAS
                     LANFLGGAYNNLGFTQTFGNSSVSQTNPNAGFYAQDEWKLSSRFTLNIGVRYDLQFLK
                     TIATDTNNVSPRAGFAWSPFESRRTVIRGSYGLFYDRIPLRALANALLSSGNTTSLGD
                     TSQVSVSLSPAQTGAPAFPNVLATLPPGVLVNFTTMNRRMQNAYSEQGSFEIEHQLGD
                     RTTVSAGYQHLRGIHLIISVNQNAPTCPASGNNNGCRPNPNYGNNSQYSSLADSYYDG
                     LHVSFVQRPARWGSYRISYTYSKALDNVGEFFFSTPIDNFNIWLDYGRSDDDQRRRVV
                     FDGTIHSSMAPAKTVWEKISHGFQLTGALQYYSALPFNITTGANTIQGTAARPTVNGA
                     FINRNAGSGFDFLNLNVRLSRVFRITERMRLEGIAEAFNALNHMNGVTLNGTFGPGAY
                     PANPLPSFRQVTAAADPRTLQLALRLSF"
     gene            complement(3473..4717)
                     /locus_tag="VFA54_03355"
     CDS             complement(3473..4717)
                     /locus_tag="VFA54_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS49870.1"
                     /translation="MNAIRAEAQHNIAKTVFPILAAISFCHFLNDMVQSLIPAIYPLL
                     KDSFHLDFGQVGLITLTYQLVASLLQPVVGLYTDRRPQPYSLTVGMGFTLVGLLFLSM
                     ASSYAIILAAAAMVGMGSSVFHPESSRVARMASGGQHGLAQSLFQVGGNAGSAVGPLL
                     AALIVLPRGQSSIAWFSLAALLAMLVLANVGAWYRTRSFAPGLSRLRHRAGQAAVSSK
                     RVAKAIAILIALIFSKYFYLASLTSYYTFYLISKFHMSVRGAQLYLFVFLGSVAAGTV
                     IGGPIGDRIGPKYVIWGSILGVLPFTLLLPYSSLFWTGVLSVIIGLTLASAFPAILVY
                     AQELVPGKVGTISGLFFGFAFGMGGVGAAILGRLADLTSIYFVYHVCSFLPAIGLLAG
                     FLPNLEKARAYPAPKAELVSAR"
     gene            complement(5040..5930)
                     /locus_tag="VFA54_03360"
     CDS             complement(5040..5930)
                     /locus_tag="VFA54_03360"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZS49871.1"
                     /translation="MAQTSRRDFLKAGVGAAVLSGIGGAAAQTARRSATDWVTLGKSN
                     VKVTRLALGTGTFGGRVQRALGQEQFTRLVRYAYDRGIRFFETSETYPGMPEMLGIAL
                     KGIPRDTYKLMTKYRTPASGDPAPKIDEFRRQLNTEYIDILLLHCLRPPTWAADYKSL
                     QDGFSEAKSRKIILAHGASVHGLPALRTFPGNQWLDIAMIRMNHNGTRMDTANTWDAD
                     APGNVDEVVAHARQVHAQGMGVISMKLCGEGRFTRAEDRDAALRFAMNLGCVDAVTIG
                     FKSTAEIDEAIERMNRVMNT"
     gene            complement(6029..7897)
                     /gene="ftsH"
                     /locus_tag="VFA54_03365"
     CDS             complement(6029..7897)
                     /gene="ftsH"
                     /locus_tag="VFA54_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZS49872.1"
                     /translation="MEPEQVKRLQKHRWKLSLVYLIVVAAILALVQIFASAPRPKAVS
                     YNEFLAEVKAGHLSEVSVTERQWIAMLKPEAVQQKGAPSVIETSRLPGINESPLIHEL
                     EAHNVKFAGHIQSGSAWTGILSWLLPLALLALVYGYGMRKLAQGPGGFLNIGKNRAKI
                     YDRSSRIGVTFADVAGVDEAKAELVEVVDFLKNPGKYQKLGGRIPKGVLLVGPPGSGK
                     TLLAQAVSGEAGVPFFSISGSEFVEMFVGVGAARVRDLFEQAKHKAPCIIFIDELDAI
                     GKSRSAGRGLILSHDEREQTLNQLLVEMDGFDASRGVIIMAATNTPEVLDPALLRAGR
                     FDRQVIVDRPDMKGREEILAVHARKIKLAPDVDLRAVAARTPGMVGADLANIVNEAAL
                     LAARRSAAQVELRDLEEAVDRVTLGLVRKSRVMSAEEKKRVAYHEAGHALVALSLPHA
                     DPVHRVSILPRSIGTLGHTLQLPEEKYIMTRPELEDRISVMLAGRACEEIVYNGVVST
                     GAADDLERASALARQMVTRFGMSEKLGNLSYGHPPRARFLPAALDGQERNYSERTAEQ
                     IDREVRRLIDSLYARSKTILENRRNDLDRVVEELMRKETLGREELERLLSAPARVA"
     gene            8190..9725
                     /locus_tag="VFA54_03370"
     CDS             8190..9725
                     /locus_tag="VFA54_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZS49873.1"
                     /translation="MERKQKLRALVRNPASAGRKTAAQSYLSEAQYRFLFDRAPLPVW
                     LVDEKTLAFVEVNNFAIQHYGYTREEFLSMTLADIRPPEDLPVFLATFQRTSQPPIRS
                     LDVGTRRWKHRKKNGTIIDVEIVTRRILFRGRPSRLTIINDITERVKAEESSAFLASI
                     VESSSDAIVGENLDGAIVTWNAGAEKLYGYQAHEVLGRPMSVLIPPEHSEDLALITNC
                     IRRGSILERHETLRLRKDGSRVEVAITVSPVKNTAGEVIGASTVARDISDRKRTEQAL
                     RDLSGRLLQIQDEERRHIARELHDSTAQRLAVLECNLRLLQQRSPGLEPAMRRNLEQS
                     VALGGECSNELRTLAYLLHPPLLDELGLASALRAYISGHNQRHGIFVSLKLPRRLPRL
                     GSDLEIAIFRIVQEALSNIHRHSKSKTAKIRLSASPCEWTLEVSDQGQGIAPDILERL
                     NEGTAILGVGIAGMRERVRQLGGRLEILSSRRGTTVRSVFPRRAPSAIAAAAPQNSPG
                     SSE"
     gene            complement(9857..10585)
                     /locus_tag="VFA54_03375"
     CDS             complement(9857..10585)
                     /locus_tag="VFA54_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS49874.1"
                     /translation="MMSVIVPVYNEQEIIRSSVTRLHEYLEQRGIEHEVILVDNGSTD
                     RTIEICRELASQRAWLRCFSIPQRGVGRAFVLGATHARGDCLVSIDADLPSELAFLDY
                     ADKLLEHCHLLVGSKFMGYQNRPLLRVLGSQLYVLIAQLCFGLTVSDFSPDSKAYRRS
                     SLAHILPHLDPWTGYVLEVALHFKRTGKRVLQIGIDVQDSRPSKFHLAHEAIYRYRHL
                     FRCWLLERRKNSWLGRDAGVQQEG"
     gene            complement(10674..12779)
                     /locus_tag="VFA54_03380"
     CDS             complement(10674..12779)
                     /locus_tag="VFA54_03380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS49875.1"
                     /translation="MQPVSEKPTVAAPAGGMPALVQWAALLLITLFLLAADQAYAWSN
                     KRPPTFDDAWYLETSLYFFHQLAQGGFSAFASAYAHSFRIKAPLISVLPLPFYFLLGP
                     GYRPAMLVNTVFLVLSSICLFLLGRRLFSPQAGLAAVIFYQTMPLSYGLSRVFMVDYG
                     LSALVIVWLYCLAVSERLTRGSVNFLLGIVLGLGLLMKVTFPAFIAGPLLVTLLAKRR
                     QPATPASESFWLWRWCAERPLAAIALPGMAVAATWYAWNLSSVLEYAWQGAYGRIGAA
                     YGAGGLSRWLLAAVNQGMSVYYTVALLIAVLAARRSAARSGPWTRGTIFVLAWLIPPF
                     LCCLVSSNREVRLALPLLPAAALLLSAAIFAGAGRPAIRAALAASAAAAPVLLYAGMS
                     FPAAARRLPTGQVGPFFLLSRDLVWAHAPDSKGDWGQQRVLDKIAELGRPNAGKRYVV
                     IGVEHSYFNANLLSYLNAYSQYPFYFTSLGYAEASVNAAVERLYSLNARFLIMTAGFP
                     PAELPPLFNRVNAGVQRQLDQGMAPFHARARIQLSPPVEAVIYQAAEPWQRFAPGSNE
                     PAPQHRTAADFSGGLRFLGFDFRSRGHYLQEITYYWAALRRIQVDKQVHVQFRRGEHQ
                     VLLQEFFVTSGEYPPTRWQPGETVRQTFFIYAPRSGDADPLEASLWLTPAAGGAPVSI
                     VAPEEWKKRSEVPLRIEEP"
     gene            complement(12840..13265)
                     /locus_tag="VFA54_03385"
     CDS             complement(12840..13265)
                     /locus_tag="VFA54_03385"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS49876.1"
                     /translation="MRYTRRELGLLLPALTTPAGLAAAKPALPSKAYRFEELPVRQNG
                     ANVSRAILRGETHKGFPIEAHETSLAPGGMPHPPHHHFHEEMFLVREGTIEVTISGKA
                     SRLGPGGVAYIASNEEHGIKNVGAAQAQYFVIALGPEEA"
     gene            complement(13326..>13688)
                     /locus_tag="VFA54_03390"
     CDS             complement(13326..>13688)
                     /locus_tag="VFA54_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZS49877.1"
                     /translation="TGEHEYTRYGFRKLLEHNAAAIWQPDIHWCGGLTELRRIAALAA
                     AYDIPVIPHTGGTFDCIHFVMATPNAPWAEMFMPPPGGPPEVYRRFEQDNQITRGPEG
                     VYARPSDRPGFGWDIEVA"
BASE COUNT         2736 a         4236 c         4252 g         2464 t
ORIGIN      
        1 cgaacaaggc atgctgccgg acgaaagatc atcactgctg caatcatgcc gcgcaaaaga
       61 cggagccggg agaccgtgcc tggactccgg cgggccgttg ccacggccag tgctctctgg
      121 cggcgggctt cgcctttagc agccaccttt ttggcattaa ccgcagcgcg gcgccaaatc
      181 atgcagttcc gtctttcgcc tggccggcgc ggcagccgga aaggcgggtt ttgtgcgcct
      241 atgccgcgtt tctctaccag cgtcctccgc cagtcctgta gccctcaaga acagcatttc
      301 tcaagaacag catttctgag gtgaagcgtt ttcatctcgt ggtttctccg gccgcaggcc
      361 cggaacagaa aggccgttcg ggccggcctt agcggtgtat ttcaaggagg actggttgct
      421 acatagaatt cgtgcgacac gcatagcagc gctaggtttt atgctgctgt ttgccgcggc
      481 cgcccgcgcg caagaaacga tcaattacgc cagcgtaagc gggcgcgtaa cagacccctc
      541 gggggcggtg gtgagcgggg cgcgcgtgac ggcgcgccaa atcgacacga accagatcag
      601 cacgaccgaa accgaccggg aagggcgctt ccggcttccc tacttaaaag ttggccagta
      661 tgagataacg atccagcagc cgggatttgc ggaggcggtt cggccgctgc ggctgaccgt
      721 gggcgccgcc tttgagctgc cgatctctct ggcagtggag tctccggcga cgaatatcac
      781 ggtgagcggc gaaggggcag tgctcgaggc ggcgcgcagc cagattgccg gcaccgtatc
      841 gcaaaccgag gtcagcagcc tgcctctgaa cgggcggaat tttctggatc tggctctgct
      901 ggttcccgga gtctctccta ccaatacagg aagcacgcag ctttttgcgg agacgtcggc
      961 ggttcccgga cagggcatct cggtcagcag ccagcggaat ttctccaaca gcttcattgt
     1021 ggacggcctt tcggctaacg atgatgcggc cgggctgagc ggcatatttt acggcctgga
     1081 cgtggtgcgc gaatttcagg tggttacgtc cggaggccag gcggagttcg ggcgcgccct
     1141 gggcggatac atcaacatgg tcaccaagag cgggaccaac acaacgcacg gcgaccttta
     1201 cgggtatttc cgcaaccagc ggttcaatgc ggccaacgcc ctttcgcata acaagctccc
     1261 cctgacccag gcgcaatatg gagcaagtct gggaggacct atcgcccgca accggacgtt
     1321 ctacttcgcc aacttcgaac agcgccaact gaatcaaagc ggcatcgtca cgatcgcgcc
     1381 ggcgaacgca agcgccatca acgcccggct cgcggaagcg gggtaccagg gcccgccgat
     1441 cgctactggc ctttatccga acccggtccg gaccactaat tttctggcca aggcagatca
     1501 cgaggtccac tcgaacgatc aattcagcgt gcgctacagt ctttacgacg tgaacagcaa
     1561 caactcgcgg ggcgtgggcg ggctgagcgc ggtgagcgcc gccgcgggat tgaatgactt
     1621 cgaccagacg gtcgcggcca gcaacatcat taccttgtcg ccgcgcacgg tcaacgaaac
     1681 gcgcgggcaa ttcacttaca gcggcctcca ggcgccgccg aacgacgctg tcggcccgtc
     1741 cgtgagcatt tccggcgtgg cttcgttcgg caggctatcg agttccccga ccgggcgact
     1801 caaccggctg tacgaaatca cggacaatgt ttcccaccag gcgggagcgc acgcacttcg
     1861 ggccggagcc aattttctct acaacgatct gaaaatcacc ttcccgcggg cggaccgggg
     1921 cagctattcg tttgcctcgc tggcgaactt tctgggaggc gcttacaaca acctgggctt
     1981 tacgcagact ttcggcaact cctcggtctc ccagacgaac ccgaacgccg gcttttacgc
     2041 tcaagacgag tggaagctga gctcgcgttt cacgctgaac ataggcgtac gctacgacct
     2101 ccaattcctg aagaccatcg cgaccgacac caacaacgtt tccccgcgcg ccggcttcgc
     2161 ctggtcgcct ttcgagtccc ggcgcacggt gattcgcgga agctatggcc tcttctatga
     2221 ccgcatcccg ctgcgcgcgc tcgcaaacgc gcttttgtcg agcggcaaca cgacgagcct
     2281 cggcgataca agccaggtga gcgtaagtct ttctccggct caaaccgggg cgccggcgtt
     2341 tcccaatgtt ctcgccactc tgccgccggg cgtgctggtc aatttcacca cgatgaaccg
     2401 gcgcatgcag aacgcttatt cggagcaggg aagctttgag attgagcacc agctcgggga
     2461 ccggaccacc gtgagcgccg gctaccagca tctgcgcggg atccatctga ttatttccgt
     2521 caaccagaat gcgcccacct gcccggcctc cggcaacaac aacggatgcc ggcccaaccc
     2581 caactacggc aacaacagcc agtattcctc tctggcggat tcatactatg acggcctgca
     2641 cgtgtcgttc gtgcagcggc cggcgcgctg ggggagctat cggatctcct acacgtattc
     2701 caaggcgctc gataacgtcg gcgagttttt cttcagcacg cctatcgaca atttcaatat
     2761 ctggctggac tacgggcgtt ccgacgacga tcagcgccgc cgcgtggttt tcgacggcac
     2821 gatccattcc tccatggcgc cggcgaagac ggtatgggag aagatcagtc acggctttca
     2881 gttgaccggg gcgttgcaat actactcggc tttgccattc aatatcacga cgggcgccaa
     2941 cacgatccag ggcacggcgg cgcggccgac ggtcaacggc gcgttcatca accggaacgc
     3001 cggaagcggc tttgatttct tgaatctgaa cgtccggctg agccgggttt ttcggatcac
     3061 ggagcgcatg cggctggaag gcatcgccga ggcattcaac gcgctcaatc acatgaacgg
     3121 cgtgactttg aacggcacgt tcggccccgg agcctacccc gccaatccat tgcctagttt
     3181 ccggcaagtc accgcggccg cggatcccag gactctccag ttggctctgc gattgagttt
     3241 ttgaatttga cacaaccagt ttggcggcga cttccctgag cggcctcctc gttgacggcg
     3301 accggacctt tgtttccccc gagcggattg ccgctactat tcatcgcgag cgagcgcgtg
     3361 ggatccgcta tttgccgttt tcaggcaaaa ctccagaagc agactcttcg ctgcgctcag
     3421 aacgacagag gatggctctg gcgcaagcgg ttccggaggc cgccgccagg cgtcaccgcg
     3481 cgcttaccaa ttcggctttc ggcgccggat acgcgcgagc cttctccagg ttaggcagga
     3541 atccggccaa caggccgatt gccgggagaa acgagcatac atggtagacg aagtaaatgc
     3601 tcgtcaggtc cgccaacctg ccgaggatgg cggccccgac gccgcccatg ccgaaggcaa
     3661 atccgaagaa caggccggag atggttccca ccttgcccgg gacgagttcc tgcgcgtaga
     3721 ccagaatcgc cgggaaggcg gaagccagcg tcaggccgat gatcacgctg agaacgcccg
     3781 tccagaacag gctggaatac ggcaacagca gcgtgaaggg aagaacaccg agaatcgaac
     3841 cccaaatcac gtacttgggc ccgatccggt cgccgatggg gcccccgatc actgtgcccg
     3901 ccgccactga acccaggaag acgaagagat aaagctgggc gccgcgcacc gacatgtgaa
     3961 acttgctgat cagataaaac gtgtagtagc tggtcagact ggccagataa aaatattttg
     4021 agaagatcag ggcgatcaga attgcgatcg ccttcgccac tcttttcgaa gagacggcag
     4081 cctgaccggc gcggtgacgc aagcgcgaca gcccgggagc aaacgaccgg gttctgtacc
     4141 aggccccgac gttcgcgagc acgagcatcg cgagcaatgc ggccagggaa aaccaggcga
     4201 tgctcgactg tcccctggga agaacgatca gggccgccag cagcggaccg acggccgagc
     4261 cggcgttgcc tccgacttga aacagcgact gagccagccc gtgctggccc cccgaggcca
     4321 tccgcgccac gcgggacgac tcggggtgaa ataccgaaga ccccatgccg accatcgcgg
     4381 ccgcggccag aatgatggcg tagctggagg ccatggagag aaacagcagc cccaccagag
     4441 tgaatcccat tcccaccgtc agggagtaag gctggggacg gcggtccgtg taaagtccca
     4501 cgacgggctg gagcagggag gcgaccaatt ggtaggtgag agtaataagg ccgacttgcc
     4561 cgaagtcgag atgaaaggaa tccttcagca gcgggtagat cgctgggatc agcgactgca
     4621 ccatgtcgtt gaggaagtga caaaaactga tggccgccag gatcggaaac acggtctttg
     4681 cgatgttgtg ttgcgcttct gcccggatcg cgttcacttt cgctccaaaa cgtcttagat
     4741 agccggtccc gccgcccgga ttcagctttt cagccgcagc agccttgcgc ccggagccat
     4801 accagtgaga ccgctggctc gcaccgtgca aaaaccggcg gttcgctcta ttcgcggcga
     4861 gccggggtgg agaagggtcc ggcaggcggc aaacaggccc gccgttggga gctgccgcga
     4921 gtcaatagca cgccgggata gcgggaattt gcggcccgga agcgggcctc gccgccgagc
     4981 cggggctgcc gatccccgca cggatccggc ggggaccgcg gctccgggag aaagggacgc
     5041 tacgtattca tgacgcggtt catgcgctcg atagcctcgt cgatctccgc ggtgctcttg
     5101 aaaccgatgg tcaccgcatc cacgcatccg agattcatgg cgaatcgcaa cgccgcgtcg
     5161 cggtcttccg cgcgggtgaa gcgcccctcg ccgcagagct tcatgctgat cacacccatg
     5221 ccctgcgcgt gaacctgcct ggcgtgcgcc acgacttcgt ccacattgcc gggcgcatcg
     5281 gcgtcccagg tattggctgt atccatccgg gttccgttgt ggttcatgcg aatcatggcg
     5341 atatcgagcc actggttgcc gggaaaggtt cgcagcgccg gcaggccgtg gacggaagcc
     5401 ccgtgagcga gaatgatttt tctgctcttg gcttcggaga aaccgtcttg caggctcttg
     5461 tagtcggcgg cccaggtcgg cgggcgcagg cagtgcagca gaaggatatc gatgtactcg
     5521 gtattgagtt gcctgcggaa ctcgtcgatt ttgggcgcag ggtcgccgga ggcgggcgtg
     5581 cggtattttg tcatgagctt ataggtatcc cggggaatgc ccttgagggc gatgccaagc
     5641 atttccggca tgccgggata cgtttcggaa gtttcaaaaa accggatgcc gcggtcgtag
     5701 gcgtatcgca cgagccgggt aaactgttcc tggcccagcg cgcgctggac gcggccgccg
     5761 aaagtgccgg ttccgagggc cagcctggtc actttcacgt tggattttcc gagcgtcacc
     5821 cagtcggtag ccgaccgtct ggctgtttgc gccgccgcgc cgccgatgcc cgacaacacc
     5881 gccgcgccaa cacccgcttt caaaaaatcg cgtctgctgg tttgagccat ggattcctcc
     5941 ctgggcagcc cgcattccgt tgagtcctgc gggcgttgat gcacgcccta taagaatatc
     6001 attgtcggag gggctgaacc aggcgttttc aggccacgcg ggcgggggcg gacaggagcc
     6061 gctcgagctc ttcccgcccc agcgtctcct tccgcatgag ctcttcgacg acgcggtcga
     6121 ggtcattccg gcgattctcc agtatggtct tggagcgggc atagagggag tcgatcaggc
     6181 gccgcacttc ccggtcgatc tgctccgcgg tgcgctcgct gtaattgcgt tcctggccgt
     6241 ccagagcggc gggtaagaac cgcgcgcgcg gggggtggcc gtaactgaga tttcccagtt
     6301 tttcgctcat gccaaaccgc gtgaccatct ggcgcgcgag agcggaggcg cgctcgagat
     6361 cgtcggctgc gccggtggac accacgccgt tgtagacgat ttcctcgcag gcgcgccctg
     6421 ccagcatcac ggaaatccgg tcttccagtt cgggccgggt catgatgtat ttttcttcgg
     6481 gcaactgcaa agtgtggccg agcgttccga tcgagcgggg aagaatcgat acccgatgga
     6541 ccggatcggc atgaggcagc gagagcgcca ccagggcatg cccggcctcg tgataagcga
     6601 cgcgcttttt ttcttccgcg ctcatcacgc ggcttttgcg gacgagcccg agcgtgaccc
     6661 ggtccacggc ctcctccaga tcgcgcagtt ccacctgagc ggccgagcgc cgcgccgcca
     6721 gcaaggccgc ttcgttgacg atgttcgcca ggtcggcccc caccatgccc ggagtgcggg
     6781 cagccacggc ccgcaggtcc acatcagggg ccagtttaat ttttcgggca tgcacggcga
     6841 ggatttcttc ccggcccttc atgtccggcc ggtccacgat cacctgccgg tcgaagcggc
     6901 cggcccgcag cagggcggga tccagcactt ccggcgtatt tgtagcggcc atgatgatga
     6961 ctcctctcga agcgtcgaag ccatccattt cgacgagcaa ctgattcaga gtctgctccc
     7021 gctcatcatg gctcagaatg aggccccggc cggcggaacg cgacttcccg atcgcgtcca
     7081 gctcatcgat gaagatgatg caaggcgcct tgtgctttgc ctgttcgaag aggtcccgca
     7141 cccgcgcggc cccgacgccc acaaacattt ccacaaattc cgatcccgaa atggaaaaga
     7201 agggaacgcc ggcctctccg ctcaccgcct gagcgagcag cgttttgccg gagcccggag
     7261 ggccgacgag caagacgccc ttgggaatgc ggccgccgag cttctggtat ttgcccggat
     7321 tcttgagaaa atcgaccacc tcgaccagtt cggctttggc ttcgtccacg ccggcaacat
     7381 ctgcgaaagt cacgccgatg cggctcgatc gatcataaat tttggcccgg tttttgccaa
     7441 tgttcagaaa accgccgggt ccctgcgcca gcttccgcat tccataaccg taaaccagcg
     7501 ccaacagggc taagggcagc agccaggaga gaatgcctgt ccaggcggag ccgctctgga
     7561 tgtggccggc gaacttgacg ttatgcgctt cgagttcgtg tataagagga ctttcattga
     7621 tgccgggcag ccggctggtc tcgatcaccg agggagcgcc tttttgctgg accgcttcgg
     7681 gtttgagcat cgcaatccat tgccgctcgg taacgctgac ttccgacaaa tgaccggcct
     7741 tcacttccgc cagaaactcg ttgtaggaaa cggccttcgg gcgaggcgcc gaggcgaaga
     7801 tctgcaccag cgccaagatg gccgccacca caatcagata gacaagcgac agcttccacc
     7861 gatgtttttg cagccgcttc acttgctcgg gttccatggt ccttgcctcc caaaacggcc
     7921 tttttctcca gtctacgacc gcggcgccgc tccggagcag gcagtggaga ccacccagcc
     7981 cagtggacgt tgccgcacct cgggcggccg ccgactccgg gccgccgctg cgtgcgccgt
     8041 ttgcaatacc gcaccggtct tctgttgaca tagttatttt gaagaggtat aagcaaaaga
     8101 cgttggtctg taacggggtt gcggtcgtgg gcggtaacct tgtgctccca aggctggtct
     8161 gatgattcag gggcagggga agtggtccat tggaaaggaa acagaagctt cgggccctag
     8221 tgcgtaatcc agccagcgcc ggccgaaaaa cggctgcgca gtcctatctc tctgaagcac
     8281 aataccggtt tctctttgac cgcgcgcccc tgccggtttg gcttgttgac gaaaagacgc
     8341 tggcctttgt ggaagttaac aactttgcaa ttcagcacta tgggtatacg cgagaagagt
     8401 tcctgagtat gacgctggcg gacatccggc cgcctgagga tcttccggta tttctggcga
     8461 ccttccagcg aaccagccaa cctccgattc gcagcctgga tgtgggcacc cgccgctgga
     8521 aacaccggaa gaagaacgga acgatcattg acgtggaaat tgtcacgcgc cggattctgt
     8581 tccgaggcag gcccagccgc ctaacgatta tcaacgacat aaccgagcgg gtgaaggccg
     8641 aggagtcctc ggcgtttctc gcatcgattg tggaatcttc gagcgacgcc attgtcgggg
     8701 aaaacctgga cggggccatc gtaacctgga acgcgggggc ggaaaagctg tacggttacc
     8761 aggcccacga ggtattgggg cgcccgatgt ccgttctcat accaccggag cattccgagg
     8821 accttgctct tattaccaat tgcatccggc ggggcagcat cctcgagcgc catgaaaccc
     8881 tgcgcctccg gaaggacgga tcgcgcgtgg aagtagccat tacggtctca ccggtcaaga
     8941 acacggccgg cgaagtcatc ggcgcctcta cggtggcccg ggatatctcc gatagaaagc
     9001 gcacggaaca ggctcttcgc gatctctcgg gccgcctgct ccagatccag gatgaagagc
     9061 gcaggcacat cgcccgcgaa ctgcacgaca gcacggccca acgcctggct gttctggaat
     9121 gcaacctccg gctgctgcaa cagagatcgc cgggcctgga accggccatg cgccggaacc
     9181 tggagcaatc agtcgcgctg ggcggcgaat gcagcaatga gctgcgcacc cttgcctacc
     9241 tgctgcaccc gccgctgctc gacgagctcg gcctcgccag cgcgcttcgg gcctacatta
     9301 gcggccacaa ccagcgccac gggattttcg tcagcctgaa gcttccccgg cgtctgcccc
     9361 gtctcggcag cgatttggag atcgcgattt tccggattgt ccaggaagct ttgagcaaca
     9421 tccaccggca ttcgaagagc aagacggcga aaatccggct gtcggcgtcc ccgtgcgaat
     9481 ggactttgga agtgtcggac caagggcagg gaatcgcccc tgatattttg gaacggctga
     9541 acgaaggaac cgcgatactg ggagtaggca tcgcgggcat gcgcgagcgc gtgcgccagc
     9601 tcggaggccg gctggaaatc ctttccagcc ggcgtggtac gacggtcaga tcggttttcc
     9661 cgcgccgggc tccttccgcc atcgccgcag ccgcgccgca gaatagcccc ggctcttccg
     9721 agtaaatctt cccggtcccg tacgacgccg aacggagccg ccgcaaaacg gcataatttc
     9781 accgcgagct tctctccctc gcgcccgccg ggctgcggcg ggcgggccag cgccggcggt
     9841 gcggcagata catggctcag ccttcttgct gaacgccagc atcgcggcct agccaggaat
     9901 tttttctccg ttcgagcagc cagcaccgga aaagatggcg gtaccggtaa attgcttcat
     9961 gagccaagtg gaatttgctc ggccgcgaat cctgcacgtc aattccgatt tggagcacgc
    10021 gcttgccggt gcgcttgaaa tgcagcgcca cctcgagtac gtagccggtc caggggtcga
    10081 ggtgcggcaa aatgtgcgcc agagatgacc ttcgataggc cttgctgtca ggcgagaaat
    10141 cggacaccgt gagcccgaag caaagctgag cgatcagcac gtaaagctgg cttcccagca
    10201 ctcgcagcag cgggcggttc tgatagccca tgaatttcga tcccaccaga aggtggcagt
    10261 gttccaacag cttgtcggcg taatccagaa aagccagctc ggacggcagg tcggcgtcga
    10321 tgctgacgag gcagtcgcct cgggcgtgtg tggcgccaag cacaaaggcc cgcccgacgc
    10381 cgcgttgggg aatggagaag caccggagcc aggcgcgctg ggaggccagt tcccggcaaa
    10441 tctcgatcgt acggtctgtc gacccgttgt ctacgaggat gacctcgtgc tcaatgccgc
    10501 gctgctccag gtattcgtgc agccgggtta ccgacgagcg gataatctct tgttcgttat
    10561 agacggggac gatcaccgac atcatggggc attgcgatta aaaggccaag agattagcat
    10621 agccgccgcc ggatcccgtg cggaaccgga gtttcagggg gcgctgccac gcgctatggt
    10681 tcttcgatcc ggaggggaac ttcggagcgc ttcttccatt cctcgggagc aacaatagat
    10741 accggcgcgc cgccggcggc gggcgtaagc caaagagaag cctccagcgg gtcggcgtct
    10801 ccgctcctgg gggcatagat gaaaaacgtc tgtctgactg tctcgcccgg ctgccagcgg
    10861 gttggcggat attcgccgct ggtgacgaag aactcctgga gaagcacttg atgttcgccg
    10921 cgccggaatt ggacgtgcac ttgtttgtca acctggattc tgcgcagggc ggcccagtaa
    10981 tatgtgatct cctgcaagta gtggccacga gagcgaaaat cgaacccaag aaaacgcaag
    11041 ccgccgctga aatcggcggc cgtccgatgt tgcggcgccg gctcgtttga tccaggcgcg
    11101 aagcgctgcc agggctcagc cgcttggtag atcaccgctt cgaccggggg tgaaagctgg
    11161 attcgggccc gcgcatgaaa cggcgccatt ccctgatcaa gctgccgctg cacgccggcg
    11221 ttcacgcgat tgaacaaggg gggcagctcg gcgggcggga aaccggcggt catgatcagg
    11281 aagcgggcgt tcaggctgta aagcctttcg accgcagcgt tgacggaagc ttcggcgtag
    11341 ccgagcgacg taaaatagaa cggatattgg gaataggcgt tcagatagct gagcaggttt
    11401 gcgttgaagt aggaatgctc cacgccgatt accacgtagc gtttgccggc gttcggccgc
    11461 ccgagctcgg cgattttatc cagcacgcgc tgctgccccc aatcgccctt cgagtcaggc
    11521 gcgtgcgccc acaccagatc gcggcttaaa aggaaaaagg gccctacctg cccagtgggc
    11581 agccggcgtg cagcggcggg gaagctcatg ccggcgtaga gcaaaacggg cgccgcggct
    11641 gcgctcgctg cgagcgccgc ccggatagcc gggcgcccgg cgccggcaaa aatcgcagcc
    11701 gacaggagta gagccgctgc cggcagcagc gggagcgcca gccggacctc ccggttgctg
    11761 ctcaccaagc agcataaaaa cggcggaatc agccaggcga gaacgaaaat cgttcctctg
    11821 gtccacgggc cggacctggc ggcgcttcgc cgcgcagcca gaacggcaat gagcaacgcc
    11881 actgtgtagt aaacagacat tccctggttg acggcggcga gcagccagcg ggacagaccg
    11941 ccggccccat aggctgcgcc gatccggccg taagcgccct gccaggcata ttcgagcacc
    12001 gagctgagat tccaggcgta ccaggttgcg gctacggcca tccccggcag cgcgatcgcc
    12061 gccagcggcc tttccgcgca ccatcgccat aaccaaaagg actcgcttgc cggggtcgcc
    12121 ggctgccggc gctttgccag cagcgtcacc aacagaggcc cggcgatgaa cgccgggaat
    12181 gtgaccttca tcaggagtcc caggcccagc acgattccca gcaagaagtt gacggagccg
    12241 cgggtgagac gctccgaaac ggccagacag tagagccaga caatgacgag cgcggaaagg
    12301 ccgtagtcca ccatgaatac gcgagataac ccgtagctta acggcatggt ctgatagaag
    12361 atcacggcgg ccagcccggc ttgcggcgaa aagagcctgc ggcccagcaa aaagagacag
    12421 atactactga gtactagaaa aaccgtatta accagcatgg ccgggcggta acccgggccc
    12481 aggaggaagt agaagggcaa cggcagcacc gagatcagcg gcgccttgat gcgaaaagaa
    12541 tgggcgtagg cggaggcgaa tgcactgaat ccgccttggg cgagctgatg aaagaaataa
    12601 aggctggttt cgagatacca ggcgtcatca aaagtcggcg gccgcttgtt ggaccaggcg
    12661 taggcttgat ctgcggccag cagaaaaagc gtgatcagca ggagagccgc ccactggacc
    12721 agggccggca tgccgccggc aggggcggcg acggtgggtt tttccgaaac cggctgcatt
    12781 acaaccccaa aatcgcattt tcccgccgcg gcggggcaat atttgaatgg gccgccggcc
    12841 tacgcttcct cggggcccag ggcaatcacg aagtactgcg cctgcgccgc gcccacgttt
    12901 ttgattccgt gctcttcatt ggaggcgatg taggcgacgc cgccgggtcc caaccgcgac
    12961 gccttgcccg aaatggtgac ttcaatcgtt ccctcccgga ccaaaaacat ttcttcatgg
    13021 aaatggtggt ggggcggatg cggcatgccg ccgggcgcca gcgaggtctc atgcgcttcg
    13081 atcgggaagc ctttgtgcgt ctcgccgcgc agaatcgccc gggagacgtt ggcgccattt
    13141 tgccggaccg gcagttcctc aaaacgatac gccttcgagg gcaaggcggg tttcgcggcc
    13201 gccaacccgg ccggggtggt caaagcgggc agcagcaggc ccaactctct tcgcgtgtac
    13261 cgcatggaac ctcctggatg gatcatggca attgtaccgc cggcgatcgt ccggaacgcc
    13321 ggcgctcagg cgacctcgat atcccagccg aacccgggac gatcggaggg gcgcgcgtag
    13381 acgccttccg gcccgcgcgt gatctgattg tcctgttcga accgccggta gacctccggc
    13441 ggccctcccg gcggcggcat gaacatctcg gcccagggcg cgttgggcgt cgccatcacg
    13501 aagtgaatac agtcgaacgt tccgccggtg tggggaatca cgggaatatc atacgccgcg
    13561 gcaagggcgg cgatgcggcg caattccgtc aatccgccgc accaatggat atccggctgc
    13621 cagatggccg cggcgttgtg ctcgagcagc ttccggaatc cgtagcgcgt gtactcgtgc
    13681 tcgccggt
//
LOCUS       DASREK010000057        11121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_702124, whole genome shotgun sequence.
ACCESSION   DASREK010000057 DASREK010000000
VERSION     DASREK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702124"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..706)
                     /locus_tag="VFA54_03395"
     CDS             complement(5..706)
                     /locus_tag="VFA54_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HZS49878.1"
                     /translation="MKNTFPGFPPETLRFLRALKRNNDREWFLKNKQVYEESVKTPMT
                     ELVLALGREMKRFAPEMMTDPARAIYRIYRDVRFSPNKMPYKTHIAALFYPRGMQKRN
                     CAGLYFHIAPEGVEIAGGVYMPEPEALLAIRGHLASQHQKFRSIIAAPEFRKLFGAMD
                     GEQLTRTPKGFAADHPAADLLRYKQFLAGVSKPPEFAASPRLFATLVQLFSGMMPFVR
                     FLNEPLRGKASLRKL"
     gene            800..1810
                     /gene="murB"
                     /locus_tag="VFA54_03400"
     CDS             800..1810
                     /gene="murB"
                     /locus_tag="VFA54_03400"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZS49879.1"
                     /translation="MDSSSSRAPANSARPLSLDSGLLPALRAIPGLALRENAPLAQFT
                     RFAIGGPADLIVETADPDSFISAIQACRRSGVPFYVIGEGSNLVVSDAGFRGVVLRYI
                     SRNITADGNLILADAGVELQRLVDFAIAHGLAGLETLAGIPGSAGAAVYGNAGAYGHS
                     ISERVAGVEFFDGDSRREFSNAQCCFEYRESIFKKHKDWMILRIRFELSPGDSAGLRR
                     RAEEIMRLRNEKFPPSMKCAGSIFKNLLVAHLPPGVAADIPPQAVREGKVASAFFLEQ
                     VGAKGLRRNGIQVASYHANLIYNAGGGTAADLRAVISDLRDRVFSRFGIRLEEEVQYL
                     GE"
     gene            complement(2086..3276)
                     /locus_tag="VFA54_03405"
     CDS             complement(2086..3276)
                     /locus_tag="VFA54_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZS49880.1"
                     /translation="MRKAAGAAALVAGGWALLWFAGEARRSHLAWSDFTVFLWKAPSA
                     ARASGAADAVPPRSGDPVQFVGLTQYLNNARAVVTHTIDDSTKYVPAAIETMDRYGIK
                     ATIFISTDRGPIEDLWPILRRAVHNGHEIGSHSRTHPCLWPPSLRFCFFHYTDYEIAG
                     SRDDILRNTDQPHVWSFAYPCGLCTKYDFVHRKLERAGYLVARNYPNEETGGHIVPDM
                     QSYDPHTYNATYTQVVQKKGGIAPTGRTDLAAINGKFDEVYGKGGIYNFMSHPQWLDY
                     GPDKFYEQHLRYVSNRQDVWYVPMGPLYAYRRVVQHTQVRRLGPDRFAATCSLDPKVF
                     PVSVTLEFRAPESWTPEADGRVLAERKAGLTDRWNEQYFRREGEKLYVTVRPSAVVVV
                     GRPE"
     gene            3328..4287
                     /locus_tag="VFA54_03410"
     CDS             3328..4287
                     /locus_tag="VFA54_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZS49881.1"
                     /translation="MRIVTEFKFTPEEVQQITAAAEDVELVLCKDHAEFLEKVADADA
                     IYGSVDKDILARAKKLRWVQAGAAGVESMAMAIKDSPVVVTNFASTFAPAISETAIGM
                     LICLTRGISRWYVPQFMARKWQPVGTVKSPDHVEISGRTMGFVGMGGIGQSLARRAYY
                     GFDMKILAVDSKPMQRPWFVSELRDPSWLMEMVPQCDVLVGAAPHTPETDRIFNEAVF
                     RAMKKTAYFLALSRGKLFDDMALVKALKEGWIAGAGLDVFPQEPPPPDHPIYDCPNVV
                     MTMHTSGWGPDRQKRLIALFVDNVRRFAKGQPLLNVVDKSKGY"
     gene            complement(4325..5407)
                     /locus_tag="VFA54_03415"
     CDS             complement(4325..5407)
                     /locus_tag="VFA54_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS49882.1"
                     /translation="MKHAITSCLLLFSAAMAADAQPLAVRPKLAVIGLVHSHVWNHLG
                     RMAAMPEANLVGIAEPNQELAAEAKKLAPNAEYFTDYKQMIEAKQPNVVWAFVENNRH
                     LEIVEYCAPRQIHVIFEKPLAATYADALRIRDLAKKYGIQVMTNYQMAWWPANYTAKA
                     QADSGAIGPVWRLHGIVGHGGPSSSGVRNKFFFDWLTDPVKNGAGALMDFGCYNALWS
                     LWYLGRPEAVYAEVNHLRPETFPKVEDSAVLVLTYKNGVGIFEASWDLPRSFQDLEVF
                     GRSGSIYMTEPKVRVWKGREEKEAPLAPLPEDRREPVTYLLSRIRSGRPLDGLVALDI
                     NVGVNEIIEAAKESVKTGKPVKLEKK"
     gene            complement(5541..6251)
                     /locus_tag="VFA54_03420"
     CDS             complement(5541..6251)
                     /locus_tag="VFA54_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZS49883.1"
                     /translation="MISEIRLARRGLLHGAALLGTGLLVSGCSKNEGGSKAGGETAEE
                     VGPGEDLMREHGVLKRVILIYRDILARLEARQDFPPDVLAGSAKLIRNFVEDYHEKLE
                     EDYLFPRFKKANRLTDLVDVLAAQHQKGRILTDTITRLATPAMLKDTEQRRSLGDAMR
                     QFIRMYEPHEAREDTVLFPEFHKIVSQHEYDALGDEFEKKENQLFGGDGFEKNVDAVA
                     GLEKTLGIYDLAQFTPNV"
     gene            6333..7217
                     /locus_tag="VFA54_03425"
     CDS             6333..7217
                     /locus_tag="VFA54_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZS49884.1"
                     /translation="MVGTSRREFLAAAALPAVGRGPGSRRMFVCLHETTSAGFGFREA
                     MEGYAKAGVRAVEVTFPKLQEFTQAESPAVARRLLADLGLTVVSCGSQTGIAEPTPAR
                     AKNLAALKAKCELAQAMGFDRMVMPSFTFERYKEDDYKRAVDNLREAGEIAKPYRLSL
                     MLEFVRTTNFIGSLPTALKLVREAGHPNVKVMLDTYHFWGGVSKFEDLELLRDGELHH
                     LHFEDTPASPPRELLEQRHRVYPGQGIAPLRRILDVLWRKKYAGPLSVELFDPVIQKT
                     DPYEIARRVRAATEPFLR"
     gene            7471..9501
                     /locus_tag="VFA54_03430"
     CDS             7471..9501
                     /locus_tag="VFA54_03430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZS49885.1"
                     /translation="MKALGGLLLLASFLLPLEAAQKPFDAGALLRLARISDPQLSPDG
                     KTVAFVAQTPDLEANTRPTRIYVVPLAGGLSQMVAAEGSQNERPRWSPDSRHIAFISD
                     RSGSSQVWIMDADGKNPRQITNLSTEASGVLLSPDGKNLIFVSSVFPDCKDDACNKAR
                     LEARAKSKVHARIYTTLLYRHWNEWNDGRRSHIFAVSVQGGAPRDLTPGDHDAPTWEL
                     GGPDGYAVSPDGKELCYAMKVEQSATSTNNDLYVVPIEGGPSKKISNNPADDESPVYS
                     PDGRYIAYRAQLRAGYESDRFRLMLHERATGTIINLTEALDRWVDAIAWSPDSSRLFF
                     TAEDRGRRPIFTIPVTGGAIRVAVGGDAYLGDMQLAPDGKTMIYSGQSASSPADIFRG
                     SSSGGSPVKLTRLNDSLLSEYRTTPLEEIWYEGAEGARVQGLVVKPPDFDPRKKYPLL
                     TLIHGGPQSAWGESWSYRWNAQVFAGAGYVVFMPNPRGSTGFGQAFIDGVNGDWGGKA
                     YQDIMNGVDRMTLEPYIDASRSGAAGASFGGYMINWILGHTDRFKALISHDGVFDLRS
                     ETLATDELWFPLWEFKGMPWENAEMYARWSPSEFITQFKTPTLVIHGEQDYRVPYGQA
                     LQLFTALQMRKVPSKLLLFPDEGHWVLKPQNSRLWYESVLAWIDQWLKGGSP"
     gene            9498..9779
                     /locus_tag="VFA54_03435"
     CDS             9498..9779
                     /locus_tag="VFA54_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49886.1"
                     /translation="MKRKAKTTLAVIIFAAAVLALIAYSTLNLGGYSCEVCIEFHGQS
                     KCRSAQGSSEQEARRTAADNACAFLASGVTDSIACTNTPPQSVRCEKKR"
     gene            9908..10441
                     /locus_tag="VFA54_03440"
     CDS             9908..10441
                     /locus_tag="VFA54_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49887.1"
                     /translation="MSRTALLLVLSLSGCALQAATLEKLTLDEMVARSTAIVRARAVT
                     ASALWIGPTIYTRTHFQVLETWKGPNAAAVDVTEPGGSIGSAAQEYSGVPHFTPGQEF
                     VLFLWTGPSGRTQVIGLSQGIFSVERPSGGAATVRRKPSGGLMLAPGAGAPVTDEEVV
                     MPLSSLAAHVHAALTPK"
     gene            10445..>11121
                     /locus_tag="VFA54_03445"
     CDS             10445..>11121
                     /locus_tag="VFA54_03445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HZS49888.1"
                     /translation="MRRWTQFPILLAWLASSAFGYYHFIHYPSRSGPYTPIYAKFDLS
                     ALVNKTVYFYVSDQVPNLAPTDSYEALIGQVRQALAVWNGVSTSDLRVGFGGITNIAS
                     YQPRTAGAEIVFDDLPPGVLGLGGPATLGAPANGFIPIIRSRVILPRDLSDPSRTSAS
                     QSFFTSLVHEIGHALGLQHTMAGSAMSMEPTRSTSRAQPLGADDVAAISLLYPAPAFN
                     SAFGSITG"
BASE COUNT         2103 a         3421 c         3387 g         2210 t
ORIGIN      
        1 ggtctcagag ctttcgcagt gaagcctttc cgcgcagcgg ctcgttcaaa aaacgcacaa
       61 acggcatcat tccggaaaag agttgaacga gcgtcgcgaa cagcctgggg cttgcggcga
      121 actccggggg cttgctgacg ccggcaagaa actgcttgta ccgaagcaag tccgccgctg
      181 gatgatcggc tgcaaaaccc ttgggcgtcc tcgtgagttg ctcgccgtcc atcgccccga
      241 aaagcttgcg aaactccggc gctgctatga tcgaccggaa cttctggtgc tgtgaggcca
      301 ggtggccacg gattgccagc aacgcctccg gctccggcat gtacacgccg ccggcgatct
      361 cgacgccctc cggcgcgata tggaagtaaa ggccggcgca gttgcgcttc tgcatgccgc
      421 gggggtaaaa aagcgccgcg atgtgcgtct tgtatggcat cttattcggg ctgaagcgga
      481 cgtcgcggta gatcctgtag atggctcgcg ctggatcggt catcatttcc ggcgcaaagc
      541 gcttcatttc gcggccaagc gccagcacga gttcggtcat gggagtcttc acgctttcct
      601 cgtaaacctg cttgttcttg agaaaccatt cgcggtcatt gtttcgtttg agggctcgga
      661 gaaagcgcag agtttctggc ggaaagcccg gaaatgtgtt cttcatggcc gaccctctat
      721 aataagtcgt gggcgacgga tcatgcgctg cgcgcaggaa acctgactcg ccacgcggcc
      781 cgtctgacta acaatgccta tggattcctc ttcttcgcgc gctccggcaa actccgcccg
      841 gcccctgtcc ctcgactccg gtttgctgcc ggctctgcgc gccataccgg gccttgccct
      901 gcgcgagaac gcgcccctcg cccaatttac gcggttcgcc atcggcggcc cggcggacct
      961 gatagtcgaa accgccgacc cggattcctt catctccgcc atacaggctt gccgccggtc
     1021 cggcgtccct ttttacgtaa tcggggaggg cagcaacctg gttgtttcgg atgcgggctt
     1081 tcgcggcgtg gtgctccgct acatctcgcg gaacatcaca gccgatggaa acctgattct
     1141 ggccgatgcg ggcgtcgaac tgcaaagact ggttgacttc gccattgccc acggtctggc
     1201 gggcctggag accctcgccg ggatacccgg ctcggccggc gcagcggttt acggaaacgc
     1261 cggagcttac ggacactcta tctcggagcg cgtggctggc gtcgaatttt ttgacggaga
     1321 ttcccggcgt gagttctcga acgcgcagtg ctgttttgag taccgggaaa gcattttcaa
     1381 aaagcacaag gattggatga tactccgcat tcgcttcgag ctcagccccg gcgattcggc
     1441 cggcctgcgg cggcgcgccg aggagatcat gcggctgcgc aacgaaaaat ttcctccctc
     1501 catgaaatgc gccggcagca ttttcaagaa tctcctcgtc gcccacctgc ctccgggggt
     1561 cgccgcagat atccctccgc aggcggttcg ggaaggcaag gtggcttccg cgttttttct
     1621 cgaacaggtc ggcgcgaagg gactgcggcg gaacgggatc caggtggcgt cgtaccacgc
     1681 caatcttatc tacaacgccg gcggcgggac ggccgcggac ttgcgcgcgg tgatcagcga
     1741 cctgcgcgac cgcgtcttca gccggtttgg cattcgcctt gaggaagagg tgcaatacct
     1801 gggcgagtag aagcggccgg ctccggaaaa cgccgaaacg cgccgaagct ttgcccgcag
     1861 gagggcagtt tcgcgcgccg ctggccagcc aggcggcctg gcattggggc ggagcttcca
     1921 cacgtcttgc ctctccgagc gcagcgagac ctccgcttct cccaggccga tttccatcga
     1981 agagaaatat ccccctgaag cgctgtttgg gacgcctgcg cgaggcagac caagaatgcg
     2041 cctccttttc gagcagtggc attaaagccg ctgcacggcg gcagcttact ccggccggcc
     2101 cacaaccacc accgcgcttg gccgcacggt aacgtaaagc ttctcgccct cgcggcggaa
     2161 atattgttca ttccagcggt cggtcaagcc tgctttgcgt tccgccaaga cgcgcccgtc
     2221 cgcttccggc gtccagcttt ccggcgcccg aaactccagc gtcacgctga ccgggaaaac
     2281 cttaggatcg aggctgcaag ttgccgcaaa gcggtcgggg ccgagacggc ggacctgggt
     2341 gtgttggacg acgcgccggt aggcatagag cggccccata ggcacgtacc agacatcctg
     2401 ccggttcgac acgtagcgca ggtgctgctc atagaacttg tccgggccat agtcgagcca
     2461 ttgcgggtgg gacatgaagt tgtagatgcc gcccttgccg tagacctcgt cgaatttgcc
     2521 attgattgcg gccaagtcgg tgcggcccgt cggagctatg ccgcccttct tctggaccac
     2581 ctgcgtgtag gtagcgttat aagtatgggg gtcgtagctc tgcatgtcgg gtacgatgtg
     2641 gccgccggtc tcttcattcg gatagttccg ggccacgaga tatcccgcgc gctcgagctt
     2701 gcggtgcaca aagtcatatt tcgtgcagag cccgcacgga taagcaaagg accagacgtg
     2761 cggctggtcc gtgttgcgga gaatatcgtc acgggagcca gcgatctcgt aatccgtata
     2821 gtggaaaaaa caaaaccgga gcgacggcgg ccagaggcac ggatgcgtgc gcgagtgcga
     2881 gccgatttcg tgcccgttgt ggacggctcg ccgaagaatc ggccagagat cctcaatcgg
     2941 accgcgatcg gtgctgatga aaatcgtcgc tttgatgcca tagcgatcca tggtttcaat
     3001 cgcggccggg acatatttcg tcgaatcgtc gatggtgtgg gtgacaacag cccgggcgtt
     3061 gttcaaatac tgcgtcaggc ccacgaactg caccggatcg ccggatcgtg gcggcacggc
     3121 gtctgcggcg cctgaagccc gcgccgcaga gggcgccttc cacagaaaca ccgtaaaatc
     3181 ggaccaggca aggtgacttc tccgtgcttc gccggcgaac cagagcagcg cccagccgcc
     3241 cgccaccaac gccgcagctc cggccgcttt acgcatttcc gtcaccgatt ggcacgccgt
     3301 ttattctaca atcagcagtc taggcctatg cgcatcgtta ccgaattcaa gttcacaccg
     3361 gaagaagtcc agcagatcac cgccgccgcc gaggacgtcg agctggtgct ctgcaaagac
     3421 cacgctgagt ttctggaaaa ggtcgctgac gccgacgcca tttacggcag cgtcgataaa
     3481 gatatattgg cgcgggcgaa aaagctgcgc tgggtgcagg ccggcgcggc cggcgtggaa
     3541 agcatggcta tggcgatcaa ggacagcccg gtcgtcgtga ccaactttgc ttccactttt
     3601 gcccccgcga tctccgaaac ggcgatcggg atgcttatct gccttacgcg cggcatcagc
     3661 cgctggtacg tgccgcaatt tatggcgcgc aagtggcagc cggtgggtac ggtgaagagc
     3721 cccgatcatg tcgagatcag cggccggacc atggggtttg tcggcatggg cggcatcggc
     3781 cagtcgcttg cgcggcgcgc ctactacgga tttgacatga agatcctggc cgttgattcc
     3841 aagccgatgc agcggccctg gttcgtctcc gagctgcgcg acccgagctg gctgatggaa
     3901 atggtcccgc aatgcgacgt gctagtgggc gcggcgcccc acactccgga gacggacaga
     3961 atcttcaacg aagccgtatt ccgcgccatg aagaaaacgg cctattttct ggccctctcg
     4021 cgcggcaagc tcttcgacga tatggcgctg gtgaaggcgc tgaaggaggg ctggatcgcc
     4081 ggagctggtt tggacgtttt cccgcaggag ccgcccccgc cggaccaccc gatctacgac
     4141 tgtcctaacg tggtgatgac catgcacacc tcaggctggg gccccgaccg gcagaagcgg
     4201 ctgatcgccc tgttcgtgga caatgtacgg cgctttgcga aaggccagcc gctgttgaac
     4261 gtggtagaca agagcaaggg gtactaggga cagcgccatt cggagcggaa atccgccttc
     4321 tcccttattt tttttccagc ttcaccggct tgcctgtctt tacggattct ttggccgctt
     4381 cgatgatttc gttcacgcct acgttgatat ccagtgcgac cagtccgtcc agcggccgcc
     4441 cggaccggat gcggctgagg agatacgtaa ccggctcgcg gcggtcttcg ggaagcggag
     4501 ctaacggcgc ttctttctct tcgcggccct tccacacacg gacctttggc tcggtcatat
     4561 aaatgctccc ggagcggccg aacacctcga ggtcctggaa gctccgcggc agatcccagc
     4621 ttgcctcgaa gatccccacg ccgtttttgt acgtcagcac cagcactgcg ctgtcctcaa
     4681 ctttggggaa agtctccggc cggaggtggt tgacttccgc ataaacggcc tccggccggc
     4741 cgagatacca gagcgaccac agcgcgttgt agcagccgaa atccatcagc gcccctgcgc
     4801 cgttctttac cgggtctgtc agccaatcga agaaaaactt gttccggacg cccgatgagc
     4861 taggaccgcc gtgccctact atgccgtgca gccgccacac gggcccgatg gccccgctat
     4921 ccgcctgcgc cttggccgtg taattggcgg gccaccacgc catctggtaa ttggtcatca
     4981 cctggatccc atatttcttt gccagatcgc ggatcctcag cgcgtccgcg tacgtggccg
     5041 ccagaggctt ttcgaaaatc acgtggattt ggcgcggcgc gcaatactct acaatttcca
     5101 aatggcggtt gttctcgacg aacgcccaga ccacgttggg ctgcttggct tcgatcatct
     5161 gcttgtaatc ggtgaagtat tcggcattcg gcgcgagctt tttggcctcg gcggccagct
     5221 cctggtttgg ctcggcgatg ccgacaagat tcgcctcagg cattgccgcc atgcggccca
     5281 gatgattcca tacgtgcgaa tggacgaggc ctatcaccgc cagcttgggg cggaccgcca
     5341 gcggctgcgc atcagcggcc atcgcggcag agaacaaaag caggcaagag gttatagcgt
     5401 gtttcatatg gtgacattct aacgctccga aaagccggga agcgggaagg gagcacgcgc
     5461 ctccgcgctc gggcgcagcc gtttgcccgt tcttgtcaga gctggcccgc ctggcccgag
     5521 ccgggtaaac gagccctgtt tcaaacgttc ggcgtgaact gggccagatc ataaatcccc
     5581 agcgtcttct ccaggcccgc gaccgcatcc acgttcttct cgaagccgtc tccgccgaaa
     5641 agctggttct cctttttctc aaattcgtcg ccgagcgcat cgtattcgtg ctgcgaaacg
     5701 atcttatgga attcagggaa cagcaccgtg tcctcgcgcg cctcgtgcgg ctcgtacatg
     5761 cggatgaact ggcgcatcgc atctccgagg ctgcgccgct gctcagtgtc tttcaacatc
     5821 gctggtgtag cgagacgagt gatggtgtcg gtcagaatcc ggcctttctg gtgctgggcg
     5881 gccagaacat caaccaggtc cgtgaggcga ttggccttct tgaagcgcgg gaaaaggtaa
     5941 tcttcctcca gcttctcgtg atagtcctcg acgaagtttc ggattagctt cgccgagccg
     6001 gcgaggacat cgggcgggaa atcctgccgc gcctccagcc gggctaatat atcgcgataa
     6061 atcaagatca ctcgcttgag aacgccgtgc tcgcgcatca gatcttcgcc gggaccaacc
     6121 tcttccgccg tttcgccgcc ggccttggag ccgccttcgt tcttcgagca gccggaaacc
     6181 agaagacccg ttccgaggag cgccgcaccg tgcagcagcc cccggcgggc caaacgaatt
     6241 tcagagatca tggaaagggc ctccttgcca aactctacca ctgattttgc tgctatcctt
     6301 tgcactgagg ctccacgccg ggagggacgg ctatggtcgg gaccagccgc cgggaattct
     6361 tagctgccgc cgcgctgcca gccgtgggcc gcggtccggg atcgcggcgc atgtttgtct
     6421 gcctgcacga gacgacctcc gccggatttg gctttcgcga ggcgatggaa ggatacgcca
     6481 aagccggcgt ccgcgcagtc gaagtcactt tccctaaatt gcaggaattc acccaggcgg
     6541 aatcgccggc cgtcgcccgg cggctgctcg cagatctggg cttgaccgta gtttcctgcg
     6601 gcagccagac gggaatcgcc gaaccaactc cggcgcgggc gaaaaatctg gccgctttga
     6661 aagccaaatg tgaattggcc caggcaatgg ggtttgaccg gatggtcatg ccgtcgttta
     6721 ctttcgagcg ctacaaagag gacgactaca agcgggccgt ggataacttg cgggaagccg
     6781 gtgaaattgc gaagccgtac aggctgtcgc tgatgctcga atttgtgcgc accacgaact
     6841 tcatcggctc gctgcccacg gctctgaagc tggtgcggga agccggccat cccaatgtga
     6901 aagtcatgct ggacacctat cacttctggg gcggcgtgag caagttcgaa gacttggaat
     6961 tgctccgcga cggagagctc catcacctgc atttcgaaga cacgccggcc agtccgcccc
     7021 gggagctgct cgagcagcgg caccgcgtct acccggggca ggggattgcg ccgctgcggc
     7081 gaattctcga tgttctatgg cgcaagaagt atgccggccc gctttcggtc gagctgttcg
     7141 atccagtcat ccaaaaaacg gatccttatg aaatcgcccg gagagtgcgc gccgctacgg
     7201 agcccttcct gcgatagcgc cgcggatacc tggaagcgcg gcattggcgg ctgcgagggc
     7261 aggagcggat tttttgagac gatattactt tcggcttcga gagtctgaac tggagttcgc
     7321 cggcggcgat gtgcttcctt aactatttga tcccaaggta cgagtgttcc aagctgtggc
     7381 gatgaatggg acgctgggag gcgcaggttg atccagtgca gtgggtcgcc ggcgatggtt
     7441 ctgctcggaa gactacagga gtgtacaaat atgaaggccc tgggaggcct gctattgctg
     7501 gcctctttcc ttctgccgct ggaagcggcg caaaagccgt ttgacgccgg agcattgctg
     7561 cgtctcgctc gcatttccga cccgcagctc tcccccgacg gaaagacagt cgcattcgtg
     7621 gcgcaaacgc cggatctgga agccaatacc cgccccacgc ggatctatgt ggtccccctg
     7681 gccggcggcc tctctcaaat ggtcgctgcg gaaggctctc aaaatgagcg gccgcgctgg
     7741 tcgcccgatt cccggcatat cgccttcatc tccgaccgct ccggctcctc gcaggtgtgg
     7801 atcatggacg ccgacgggaa gaatccgcgc cagatcacga atctttcaac cgaagccagc
     7861 ggcgtgctgc tctcgccgga tggcaagaat ctgatttttg tcagctctgt gtttcccgat
     7921 tgcaaggacg acgcgtgcaa caaggcccgc ctcgaggcgc gcgccaaaag caaggtccac
     7981 gcccgaatct acactacgct gctctatcgc cactggaacg aatggaacga cggccgccgc
     8041 agccatattt tcgcagtctc tgtgcaaggc ggcgcgccgc gcgatctgac gccgggcgac
     8101 cacgatgcgc ccacttggga actgggcggg cccgatggct acgccgtctc gccggacggc
     8161 aaagaacttt gctacgccat gaaggttgag caatcggcga ccagcaccaa taacgatctc
     8221 tacgtagtcc cgatagaggg cggcccgagc aaaaagatct ccaacaaccc ggccgacgac
     8281 gaatcccccg tttactcgcc ggacggccgg tacatcgcct accgcgcgca gcttcgggcg
     8341 ggctacgaga gcgaccgctt tcgcctcatg ttgcatgagc gcgccaccgg gaccattatt
     8401 aacctgaccg aggcgctgga ccgatgggtg gatgcaatcg cctggtcccc agactcgagc
     8461 cgcctgtttt ttacggccga agatcgcggg cggcggccga tcttcaccat tccggtaacc
     8521 ggcggcgcga ttcgagtggc cgtgggcgga gacgcttacc tgggggacat gcaactggcg
     8581 ccggacggca aaactatgat ttacagcggc cagagcgcgt cgagccccgc ggatatcttt
     8641 cgcggctctt cctccggcgg ctccccggtg aagctgaccc gcctcaatga cagcctgctc
     8701 agcgaatacc gcacgacgcc cctcgaagag atctggtacg aaggcgccga aggcgcccgc
     8761 gtccaggggc tggtggtcaa gccgcccgac ttcgaccccc ggaaaaaata tcctctgctg
     8821 acgctcatcc acggcgggcc gcagtcggcg tggggcgaga gctggagcta ccgctggaac
     8881 gcgcaggtgt tcgccggcgc tggctatgta gtattcatgc caaatccgcg gggatccacc
     8941 ggcttcgggc aggcgttcat tgacggcgtc aacggcgatt ggggcggcaa ggcctatcag
     9001 gacattatga acggcgtaga tcgaatgacc ctggagccgt acatcgacgc cagccgctcg
     9061 ggcgccgccg gagcttcttt cggcggctat atgatcaact ggatcctcgg gcacaccgat
     9121 cgcttcaagg ctctgatctc ccatgatggt gtgtttgacc tgcgcagcga gacgctggcc
     9181 accgatgagc tgtggtttcc gctttgggaa ttcaaaggaa tgccttggga aaatgctgaa
     9241 atgtatgcgc gctggtcgcc ctcggagttt atcacgcagt tcaagacgcc gaccctggtc
     9301 atccacggcg agcaggatta ccgggtgcca tacggccagg cgctccagct tttcaccgcg
     9361 cttcaaatga ggaaggttcc gtccaagctg cttttgtttc ccgacgaggg ccactgggtt
     9421 ctcaagccgc agaacagccg gctttggtac gagagcgttc tggcctggat agatcaatgg
     9481 ctgaaaggcg gaagcccatg aagcggaagg caaagacgac gctggcggtg atcatttttg
     9541 cggcggcggt attggctttg atcgcgtact cgacgctgaa tctcggcgga tacagttgcg
     9601 aggtttgcat tgagtttcac ggccaatcca aatgccggag cgcccaggga tcgagcgagc
     9661 aagaggcccg ccgaaccgcc gccgacaacg cgtgcgcctt tctggcctcc ggcgttaccg
     9721 acagcatagc ctgcacaaat acccctcctc agagcgtccg gtgcgagaaa aagcggtagg
     9781 gaagggcggg gaggcttgcc ccagaggtgt gtccgggcgg ctccgcagaa tctgtgtgaa
     9841 tcctaacgac ctctcgattt ttcaacaact tcaggttggc ctgaagattg cgttaaatcg
     9901 tacggaaatg agccgaactg ccttattgct cgttttgtcc ctctcagggt gcgcattgca
     9961 ggcagccact ttggaaaagc tgaccctgga tgaaatggtc gcccggtcta ccgccatcgt
    10021 ccgcgcccgg gcagtgactg cttcggccct gtggatcggt ccaacaattt acacccggac
    10081 tcattttcag gtgctcgaaa cctggaaagg cccgaacgct gcggcagtgg acgtaacaga
    10141 gccgggcggg agcattggat cagccgcaca ggagtactcc ggagtgccgc acttcacccc
    10201 gggccaggaa ttcgtgctgt ttctgtggac cggcccgagc ggcaggaccc aggtaatcgg
    10261 tctttctcag ggcatcttca gcgtagagcg cccctcgggc ggcgcggcta cggtgcggcg
    10321 caagccaagc ggcggcttga tgctggcgcc cggcgccggc gctcccgtga cggacgagga
    10381 agtagtcatg ccgctttcat cgctggctgc tcacgtccac gcggctttga cgccgaaata
    10441 gcgcatgcgg cgttggactc aatttccgat tctgctggcc tggctggcct cgagtgcgtt
    10501 tgggtattac cacttcattc attacccttc tcgatccggc ccctacacgc ccatctatgc
    10561 gaagttcgat ctgagcgcgc tggtcaacaa aaccgtctac ttttacgttt cggatcaggt
    10621 tccgaaccta gcgccgacgg acagctatga ggcgctcata gggcaggtcc ggcaagccct
    10681 cgccgtgtgg aacggcgttt ctacatccga tctgcgggtc ggttttggcg ggataacgaa
    10741 tatcgcctcg tatcagccgc ggacagccgg cgctgagatc gtgtttgacg acctgcctcc
    10801 gggggtattg ggccttggcg gaccggccac gttgggcgct ccagccaacg gattcattcc
    10861 tatcatccga tcgcgagtga ttttgccccg cgatcttagc gacccgagcc gcaccagcgc
    10921 cagccaatcg ttttttacca gcctcgttca cgaaatcggg catgccttag ggttgcagca
    10981 caccatggcc ggctcagcca tgtcgatgga gcccacccgc agcacgagcc gggcgcaacc
    11041 gctcggggcc gatgacgtcg ccgcgatttc gctgctgtat cccgcgccgg cattcaattc
    11101 ggcattcggg tcgattacgg g
//
LOCUS       DASREK010000058         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1188774, whole genome shotgun sequence.
ACCESSION   DASREK010000058 DASREK010000000
VERSION     DASREK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188774"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1014..2054
                     /locus_tag="VFA54_03450"
     CDS             1014..2054
                     /locus_tag="VFA54_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS49889.1"
                     /translation="MNIAQVSPLYERVPPRMYGGTERVVSYLTEELVQMGHDVTLFAS
                     GDSITKARLIAPCPEALRLNVNCIDQMAHHMVMLEWVLQRAAEFDIIHYHTDYFHFPF
                     SKRQRTPSVTTLHGRLDIPDLVPLYREFRELPLVSISNAQRQPLPWANWVGTVYHGLP
                     RHLYTLHEEPGEYLAFLGRISPEKGVDRAIEIAKRAGIPLKISAKVDKADYEYYCEHI
                     KRLLDHPLIEFIGEIGEGEKNDFLGNAYAFLFPINWPEPFGIAMIESMACGTPVVAFR
                     RGSVPEVIEEGVTGLIVDSIEEAVRALNRIPALDRACCRRRFEQRFDACRMARDYINI
                     YERLVDRRQAAA"
     gene            2067..4244
                     /locus_tag="VFA54_03455"
     CDS             2067..4244
                     /locus_tag="VFA54_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HZS49890.1"
                     /translation="MEVIRVKDQYYILAVSSLADVRTRVLKHGDTFAVFDRYGDIQPV
                     GLGEQGIYHEGTRFLSQLVLTLGMGRPLLLGSTVRQDNLLLAVDLTNPDVTVNGKIVL
                     PRGTLHIYRTKFLWQSRCYERFRIWNYGLDAVDFSFSLQLDADYADIFEVRGLKRQRK
                     GHFLESKTDENGILFGYEGLDRVLRWTRIEATPKPSAVSESTLSFSTRLAPKAEATFF
                     ITLTCESDPSEAAEPQSECRSYACGIEEATVALKKARSDDCEIHTSNEQFNDWLNRSA
                     ADLHMMLTSMPTGPYPYAGVPWFSTPFGRDGIITALEVLWFKPEIARGVLSYLAATQA
                     IEKAPEQDAEPGKILHEARQGEMAHLGEVPFRRYYGSVDATPLFVMLAGAYYQRTGDR
                     PFIESIWPNITLALKWIDNYGDRDQDGFVEYFRQSSKGLVQQGWKDSQDSIFYEDGRL
                     AEGPIALCEVQGYVYAAKRYASDLAAMLGCQEQAAELSRQALELRERFEKAFWCEDLN
                     TYALALDGRKRPCRVRTSNAGHCLFTGIAEPEHARAVATTLLEPISFSGWGIRTVASS
                     EARYNPMSYHNGSVWPHDNALIAAGLARYGLNEAAGKVLTGLFDSSLFLELHRLPELF
                     CGFARGVGKGPTLYPVACSPQAWAAASVFLILQACLGMTFDQPKRRIYFHHPYLPPYL
                     QKMQIRNLRVGDTIVDLSLERYLHVVGVNVLRREGQIDVISIK"
BASE COUNT          851 a         1257 c         1309 g          895 t
ORIGIN      
        1 agaatcgtct catggttgag cctcctgaaa cttggtattg gttcttctcg gaacccaatc
       61 caggctacca gaggctcaac ctgctaccct tcccgctgcc gttccgggtt caaagctaat
      121 ttggacaaga gtgattcgcc agcagcgtat cggctaacgc gccatcgctc ccgcagaact
      181 cttcttcttc aaaccttttt gattttttct cgcgtgttct ttcctaaggc atgtggccca
      241 taccgtgctg cttgtataga acccggcggg cccaggcgcc tgccggcggt catccaggta
      301 tttggaggaa ttatggcacg cagatcacaa accagcggcc ggcgaaccag cgccagcaag
      361 tcgagcaagt ccaagtcaac cacgtcgcgc cgcggcggtt cgagtgcgcc gactcgccgc
      421 agagcatcaa agagctcgac ggcccggctt cctcgcagct ccaaaacggc ggtgcgggcc
      481 gggggaaaac gcggcgcgcg agaggagccc gggggcggaa tggaccttga agaatgacgc
      541 gcggagacgt tggctcgaaa aacctgtcct gcaagaaata gctccacaat gagtaacgcg
      601 cgcgctcgag atgggaccac gatggcggac tgccaagtat tacattctcc tctctccacc
      661 ggcgtttcga tggccatctt ctcgaacagg ccaatgtcga ggtcccgttt tcctttgcgc
      721 ggccgtttct ggcggctcgc cgccggcggt acggaaggat ccggttcgga agcagccgtg
      781 acaggcgttg cgtccggggc tgtgcgactg gtgtaacttc cgattcgcaa atcaggaaaa
      841 cttttcgcga agatttttcg cgatgcgccg ccgcaggagg cgagccggta acggtggctc
      901 gcggtgaggg cggcttcgtg cgaggctctc ccggccgccc gctccgcggc agggcgggcc
      961 aggcccgtca tttggcatta tgtttgtaaa ggacgtaacg aggagggttt cgattgaaca
     1021 tcgcccaggt gtcgcccttg tatgagagag taccgccgag aatgtatggc ggaacggaac
     1081 gggttgtttc ttatttaaca gaagagttgg ttcagatggg ccatgatgtc acattgttcg
     1141 ccagcggaga ttccattacg aaggcgcgcc tgatcgcccc ttgcccggag gcgctccgcc
     1201 tcaatgtcaa ctgcatcgac caaatggcgc accatatggt tatgctggag tgggtgctcc
     1261 agagggcggc ggagttcgat atcattcact accacacgga ctactttcat ttcccgtttt
     1321 ccaagcgcca gcggaccccc tccgttacaa ctctgcacgg gcggcttgac ataccggatc
     1381 tggtccctct gtatcgcgag tttcgcgaac tgccgctggt ctccatctcg aatgcccagc
     1441 ggcagcccct gccttgggcg aactgggtag gaaccgttta tcacggcttg cccagacatc
     1501 tttacacatt gcacgaggag ccgggagagt atctcgcctt tctgggccgc atttcgccgg
     1561 aaaaaggggt ggaccgcgcg atcgaaatcg ccaaacgcgc gggaattccg ctaaagattt
     1621 cggcgaaggt tgataaggcc gactacgagt attactgcga gcacatcaaa aggctgctcg
     1681 atcatccgct gattgaattc atcggggaga tcggcgaggg ggaaaagaac gactttctcg
     1741 gcaacgcgta tgcgttcttg tttccgatca actggcccga gccctttggc attgcaatga
     1801 tcgaatcgat ggcctgcgga accccggtag tcgcattccg ccgcggatcg gttccggagg
     1861 tcatcgagga aggagtaacc ggtttgatcg tcgacagcat cgaagaagca gtcagggcgt
     1921 tgaaccggat tcctgctctg gatcgcgcct gctgccgccg gcggttcgag cagcggttcg
     1981 acgcctgccg catggccagg gattatataa atatctacga gcggcttgta gaccgaaggc
     2041 aggcggcggc ctgagaaggg tttgccttgg aagtcattcg ggtaaaagat caatattaca
     2101 tactggcagt atcttcattg gcggacgtca ggacccgcgt actgaagcac ggcgacacgt
     2161 tcgctgtttt cgaccgctat ggagacatcc agcccgttgg cctcggcgaa cagggcatct
     2221 atcacgaagg gacgcgcttt ctttcccagc tcgtgctaac cctcggcatg ggacgtccgc
     2281 tgctgctcgg ctccacggtg cggcaggaca atcttctgct ggccgtggat ttgaccaatc
     2341 ccgacgtcac cgtcaatgga aagattgttc tgcctcgcgg aaccctgcat atctaccgga
     2401 cgaaattttt gtggcagagc cgctgttatg agcgcttccg catctggaac tacggcttgg
     2461 acgccgtgga tttttccttt tccctgcaac tggacgccga ttacgcggat atcttcgagg
     2521 tgcgcggttt gaagcggcag cgtaaagggc attttttgga aagcaagacc gacgaaaacg
     2581 gaattctgtt tggctacgag gggcttgacc gggtgctgcg ctggacccgc atcgaggcca
     2641 cgcccaagcc aagcgcggtc agcgagtcca cgctcagctt ttccacgcgg ctggccccca
     2701 aggcggaggc gaccttcttc atcacactca cttgcgagag cgatccttcc gaggcggccg
     2761 aaccgcagtc cgagtgccgc tcgtatgcct gcggaatcga agaagccacg gtggcgctga
     2821 agaaagccag gagcgacgat tgcgagatcc acacgtccaa cgagcagttc aacgactggc
     2881 tgaatcgttc cgcagcggac ctccacatga tgctcaccag catgcccacc ggaccgtatc
     2941 catatgccgg ggtgccgtgg ttcagcacgc cctttgggcg cgacggcatc atcacggccc
     3001 tggaagtcct gtggtttaag cctgaaattg cccgcggcgt gctgtcgtat ctggctgcga
     3061 cgcaggcgat cgagaaggcg ccggagcagg atgccgagcc gggaaagatc ctgcatgaag
     3121 cgcgccaggg cgagatggct cacctcggcg aagtgccgtt ccgcagatat tacggcagcg
     3181 tggacgccac gcctttgttt gtaatgctcg ccggcgccta ttaccagcgg acgggcgacc
     3241 ggccgtttat cgaatccatc tggcccaaca tcacactggc gctgaagtgg atcgacaact
     3301 acggcgaccg cgaccaggac ggcttcgtcg agtatttccg gcaatcctcg aagggattgg
     3361 tccagcaggg ctggaaagat tcgcaggact cgatctttta cgaggatggg cggctggcgg
     3421 aagggccaat cgcgctttgc gaggttcagg gttatgttta cgccgccaag cgctacgcct
     3481 cggatttggc agccatgctg ggctgccagg agcaggcggc cgaactgtcg cggcaggcgc
     3541 tcgagctgcg ggagcggttc gaaaaagcct tctggtgcga ggacctcaat acgtacgcgc
     3601 tggcgctgga cggccggaag aggccctgcc gcgtgcgcac ttccaatgcg ggccattgcc
     3661 tattcaccgg gatcgccgag ccggagcatg cgcgcgcggt ggccacaacg cttctcgagc
     3721 cgatttcgtt ctccgggtgg ggcatccgga ccgttgcgtc ttccgaggcg cgctataacc
     3781 cgatgtccta ccataacggc tccgtctggc cgcacgacaa tgcgctcatc gcggccggac
     3841 tggcccgata tgggctgaac gaggccgcgg gcaaggtttt gaccggactc tttgattctt
     3901 ccttattcct cgaactgcac cggctgcctg agctgttttg cggcttcgcg cgcggggtag
     3961 gaaaggggcc gacgctctat ccggttgcct gttccccgca ggcctgggcg gcggcttctg
     4021 tattcttaat acttcaagcc tgcctgggga tgaccttcga ccagcccaag cggcggatct
     4081 acttccatca cccgtacctg ccgccgtatt tgcaaaaaat gcaaatccgc aacttgaggg
     4141 tcggcgatac catcgtggac ttatcgcttg agcgttacct gcacgtagtg ggcgtcaacg
     4201 tgctccggcg ggaagggcag attgacgtta tcagtatcaa ataaacatct ggggcaagac
     4261 cgaactccgc ttaattcagg ccggcggcca tgctcggcgc aggcttgtct gc
//
LOCUS       DASREK010000059        20356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_364680, whole genome shotgun sequence.
ACCESSION   DASREK010000059 DASREK010000000
VERSION     DASREK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 20356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20356
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364680"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2510)
                     /locus_tag="VFA54_03460"
     CDS             complement(<1..2510)
                     /locus_tag="VFA54_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49891.1"
                     /translation="MNAKFLRSAVGARVLAALCAMTGLLCLPLRAQVTTADILGTVTD
                     PSGAVLPGVRVTAENLDTSATRAAVSDSTGNYLIPLLPPGPYSVRAEMQGFKTFTVPR
                     VVLAVGDRLRVDVRLEVGQLEQSVEVTAQTPALQSEKSSVGTLVNDRAVEDLPLNGRN
                     FVTLARLAAGATEGVNNALSSGNRPDDRRRASSVAVNGQPDTNNNFLIDGMDDNERFI
                     GTIIVKPSMDALAEFRVETNLYSAEIGRTAGGVINLITKSGTNDLHGSLFEFFRNEHL
                     DAKNFFAGPGPTPAYKQNQFGGSLGGPIKKNKTFFFGDYEGFRLRQGQTFTSTVPTLA
                     MRQGNFAGVNPIFDPTTTRSDPSRPGVSIRDRFPNDQIPPSLIDPVAAKVVALYPIPQ
                     TGGLVNNFTQNPAKTQRDDTFDAKIDQHFSDRDIFFARYSFNDTNTFLPPQLPKIGNI
                     QAGGDTAQFAGASRQRAQGLHLNYVHTFRPNLLAEFKAGYARYAIHTLPPNYGNNVDQ
                     QLGIPGGNVDADSSGLTQMVIAGFRGLGDSNFIPIITINNVFQYVGSVTYNRSGHNIK
                     TGTDIRRRQTSPFQSPQARGQFSFDGNFTNDPSGDVARSGNSIASLLLGYPSATVRSK
                     YLVVPGFRNTEFAVYVQDDWRVTQWLTLNLGLRYEIFTPQHEVANRISNVDLAAGKII
                     IAGQNGVSGSAGVPGDYNNLGPRFGFAATVTPRTVVRGGFGINYYPIFFGSTSALRNP
                     PFVSLYSVSTTPLTPINRISDGFPLPAPTDPLNPTGNLTAMALDLRNPYVEQFNLMVQ
                     REIVSGTVWNIGYVGALTRKDYIAPNVNFDLPGPGAIQP"
     gene            complement(2863..5031)
                     /locus_tag="VFA54_03465"
     CDS             complement(2863..5031)
                     /locus_tag="VFA54_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZS49892.1"
                     /translation="MPNFRAVLVAAFHLLAAYGFASQPIARQAQREWPVYGGGPESIR
                     YSSLKQIDRSNVRRLEMAWRFDTGDEFSGSEMQCNPIIVNGVLYATTPKLRVIALDAA
                     SGKLRWSFDPSQGRKVLGKARNRGVTYWSDGVDERIFFAARQYLYALDARNGRLISSF
                     GREGRIDLRENLGRNPQQMSISATSPGIVYQDLLIIGSIVSETLPSSPGDIRAYDVRT
                     GALRWSFHTIPHPGEFGYETWPKDAWQYIGGANNWAGMSLDVKRGIVFAPTGSAAFDF
                     YGANRLGDNLFANCLLALDARTGKRLWHFQSVHHDLWDRDFPSPPSLVTVRRGGRLVD
                     AVAQTTKSGHVFLFERETGKPLFPIELRKYPASDVDGEAAAAMQPLPTVPPPFARQML
                     TEAMLTRRTPEAHQAVLERFRKLRSAGQFVPPSREGTIIFPGFDGGAEWGGSAFDPET
                     GILYVNSNEMAWILRLVERPRGAAQTSGRELYQRNCASCHRDDLHGTPPEFPSLEHLS
                     DRFNEADVYAIIRQGAGRMPAFANLPQPAVRALARYLLYGEDWQVQNSTEPSPIDLKY
                     GIDGYNRFLDPEGYPAVEPPWGTLNAIDLNTGKIAWQVPLGEYPALAEKGMRNTGSEN
                     YGGPVVTAGGLVFIGATNFDKKFRAFDKKTGALLWETTLPAAGNATPAVYEAGGREFV
                     IIACGGGKSGAPSGGTYVAFALPQSLLPARTRNSHTEKRP"
     gene            5186..5677
                     /locus_tag="VFA54_03470"
     CDS             5186..5677
                     /locus_tag="VFA54_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZS49893.1"
                     /translation="MRFRIAFALLCAPFFCARGLFAAEPEPPRQPIPYSHKTHAAAGL
                     QCRDCHEMPGAGKAAGNPKEERCMGCHATVKRESPAIQKLAQYYKDRKPVPWARVYQI
                     PDYVFFSHKAHLSRQGISCETCHGPVAQRDVITMEKPVNMADCMFCHDQWRVPIACNA
                     CHE"
     gene            5785..7083
                     /locus_tag="VFA54_03475"
     CDS             5785..7083
                     /locus_tag="VFA54_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZS49894.1"
                     /translation="MRLPKLLRVKQHFPSRVLADVGGAVRKELESAAWTWQVKPGSRI
                     AIGVGSRGITNIAMIVKAVVDFWKSRGMRPFIIPVMGSHGAATAEGQADVLAHYGIIE
                     QTMGAPIISSLDVVPLGPTPEGIEVVMDRTAFESDGVMLVSRIKWHTDFAGKLESGVH
                     KMMAIGLGKYAGAQHYHTFAVRLGHERVIRSVGRKVLATGKMLGGLAILEDAYHNTSE
                     VHALGVKGMERREEELLERVKSWAPRIPAPEIDLLIVDEMGKNISGSGMDTKIVNRSV
                     TGDNNVWPNAPKIMRIFVRDLTALSYGNAVGLGMADVITDKLYNKIDRVPTELNALTA
                     STTPMARTPIHYPTDRMCLEKVVPTLGRIDTMEATIAWIRNTLELGELMLSENLRDEI
                     RRNSMLEIVGGPAPIEFHPDGSLVELLGAPQHAPEALTHR"
     gene            7101..7937
                     /gene="uppP"
                     /locus_tag="VFA54_03480"
     CDS             7101..7937
                     /gene="uppP"
                     /locus_tag="VFA54_03480"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HZS49895.1"
                     /translation="MPLYQAVVLAIVQGITEFLPISSTAHLALVPWLMGWRDPGLTFD
                     VALHLGTLAAVLVYFVRTWIRLLSQALGRRGDPKDDFSANPRLFWFLVVATIPGGVAG
                     LALERYAESTLRSPYVIAATMIGVGLLLWWSEMKGRLQKDLDAVSLADAAAIGTAQAF
                     AVVPGVSRSGITIAAGLFRNLERAAAAQFSFLLATPIIGGAALKAAWNIHRSGGLAPG
                     MRTPFALGILVSAVTGYAAIAFLLRYLQARTLKFFVWYRVICGIMILALALFFRDSVM
                     HP"
     gene            7983..10331
                     /locus_tag="VFA54_03485"
     CDS             7983..10331
                     /locus_tag="VFA54_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZS49896.1"
                     /translation="MRFLSPTSRRRVNELVGLLLLLSGIALGLSLESYSPLDPSWNTA
                     SPASRPENLLGYPGSYTADLAFQLLGLGAFLLPLLMLALSWKWIRSEPLDVSKLIGAF
                     LLVVNCCAALTVFRPAFLWKDTIEIGGLTGRVTADLLLSNLNLTGTLIAIAIGGLLSL
                     YLMTAFSLERFGGWLKRRFAFLGKWRKRYGRWRQEVKERRRREEAARSEALARRRIDR
                     EAEVKDASGSGRVAKNEPPEAPVVELPAPVAEPAAAFAEDPPIRELEPAPAPERHRAP
                     RRTGPARIAAANFKIPPTSLLEPPAGRNPYDEEELRQLAVMIRSKFEEFNVHGAVTQI
                     NPGPVVTTFEFKPEAGVKYSRITNLAEDLCLGLQAESILIERIPGKSTVGIEVPNRRR
                     EVISLREIIESPEFQDSGSHLTFSLGKDINGRIRTANIESMPHLLIAGSTGSGKSVLM
                     NCLIMSILYKATPDEVRFIMVDPKRLEMGLYEDIPHLLTPVITDLKKASYALRNATIE
                     MERRLKLLATQGVRNIDQYNKKVRAQAYKELSLFEEDEAAEPAEPLPYILILIDELAD
                     LMLLEGNNVEESITRLAQMARAVGIHLVLATQRPSVDVITGLIKANFPARISFRVATR
                     VDSRTILDSMGAEALLGRGDMLFLPPGSSRMIRVHGPYVSESEITEVVKFWKEQSKAA
                     YETSYLAPPPKEEDGKESEDEAEFAGEEDPMYEDAVRLVLEMGKASTSILQRRLRLGY
                     GRAARILDVMERDGIIGPPDGSRPREVLKRPDWLLEVDNSLR"
     gene            complement(10346..11740)
                     /locus_tag="VFA54_03490"
     CDS             complement(10346..11740)
                     /locus_tag="VFA54_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49897.1"
                     /translation="MRAALLFLPCLTALFLASASAAQTIKEDNYPLRASCSDAAETVA
                     LLKRGDPVRIRFSLTGNAKPCYAVSVETGGKTLQGYVPADGLTNLEAYEAKRRSDSAA
                     AARALPVISRSEVESAREKAVEEQETGKTNYALAAQLNLAADALQSGRPADAERILEK
                     APAPPGQRAVALLRAYALLQLNQPDRALDVLEPALRTNKKDPQLFAAAGRAAYNLDDL
                     PRALAYLKESLELKPDETLQKIYARIEREMSADKSGEKSYGMRFLLRYDNAVASPELA
                     RSMVDVLEQEFSRISFQLGCPADERIITIVQSREAYRQTTGAAEWSGGVYDGKIRIPL
                     ARTATIDPETRQTFAHEIVHACMANIGRWPTWLHEGVAQKLSGDALRPVQRDMLKTLA
                     RVGQLPKLEDLGNSWSGLNTKQAAIAYTLALAATELFVQQHGDMALRNLMNNPQSLNQ
                     ITPELDKALAASLQ"
     gene            complement(11796..12428)
                     /locus_tag="VFA54_03495"
     CDS             complement(11796..12428)
                     /locus_tag="VFA54_03495"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZS49898.1"
                     /translation="MFTGIIEETGIVETPGSKLAVRADRVCSDLTAGASISVNGVCLT
                     AVQVKGPIFFADLSPETLARTNLGDLRRGDPVNLERPLSPNGRISGHIVQGHVDGTGE
                     LVSLESLRDGNWWLKVRAPRDLERYLVFKGSIAIDGISLTIAALEGGELSVAIIPHTY
                     SATNLSHRRPGDRLNLECDIIAKHVEKLLQAMDLKPGRRLTADRLREEGF"
     gene            complement(12708..13991)
                     /gene="aroA"
                     /locus_tag="VFA54_03500"
     CDS             complement(12708..13991)
                     /gene="aroA"
                     /locus_tag="VFA54_03500"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZS49899.1"
                     /translation="MDKIIFPAQRLAGVVRLPGDKSISHRYAMLAAIAEGVSRLENFS
                     TGADCASTLACLRELGVPIASEKATVSITGVGLSGLQAAARPLDAGNSGSTIRMLSGI
                     LAAQPFQSEISGDASLKRRPMGRILDPLTKMGAKIEAWEGNFPPLRIQGAPLRGIDYE
                     LPLPSAQVKTAILLAGLYARGPTTVREPVSTRDHTEIALREFGAAIQVQGQCIRLEPR
                     PALRAGSFFVPSDLSSAAFFLVAALLVPGSDVVIKGVGLNRTRSALLDVLLSMGAELA
                     ISQVRHVHGEPVGDVAVRSSPIRGGVIQGATTAAVIDEIPVLAVLGASSENGLVIENA
                     AELRVKESDRIATVADNLGRMGVKARAWQDGLEIPGKSRFRAAEIDSFGDHRIAMAFA
                     VAALAADGPSVIHRAEAASVSFPEFYQILDTLRAD"
     gene            complement(14252..14446)
                     /locus_tag="VFA54_03505"
     CDS             complement(14252..14446)
                     /locus_tag="VFA54_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49900.1"
                     /translation="MKNAVLRKMIEQLRRELQEISYALWSLEALARGKPRRGRPPKIA
                     GEIKEKRRKKRRSGVGPAKK"
     gene            14604..14990
                     /locus_tag="VFA54_03510"
     CDS             14604..14990
                     /locus_tag="VFA54_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZS49901.1"
                     /translation="MFLKEEKTTRDATYQDPHLIIHFQRQRVELDGATLTLTRKEYDL
                     LAMLSQNAGEIVPRDVLLRQVWGYCEGIRTRTLDVHIRRLRKKLEPFSDRYIETIFGI
                     GYRFQPFRPRTAPYEMQKRMAVSAIA"
     gene            15127..16317
                     /locus_tag="VFA54_03515"
     CDS             15127..16317
                     /locus_tag="VFA54_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS49902.1"
                     /translation="MDSFIYALVMVPALRQLLPRSHIPPSAANIGFYGGLLFALFLVG
                     WGFAFVWGPIADRFGRVPTLMLTILCYSLFTFLGAGAANVWQLAAYRLLAGLGIGGEW
                     AMGGTFVAEEWPEDRRKMASAYMHTGYYFGVLLAGAANSIIGANYGWRAMFALGGTPA
                     LLVAFIRYGVNEPQRWEVQEKPSMRKAFFALFSPEYRRRTLLNSVYLLVSIVGLWAGS
                     VYVPAAVAQVAAREGFSPRQGTQFASYGTILLSLGTILGCLILPALAEKLGRRAALAF
                     YFALMLIFIPVSFGYVFYLRQGALLWFLVCLFFLGVGGANFGMYTLWLPEQYRTECRA
                     SAFAFSTSVGRFIGAGITFLVGAGVSHFHTLGIPVALTGAAFAAGILLLPLGVETRGK
                     PLPA"
     gene            complement(16325..16720)
                     /locus_tag="VFA54_03520"
     CDS             complement(16325..16720)
                     /locus_tag="VFA54_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49903.1"
                     /translation="MTRRLFLAVCLAALSGQARPGQKPKREPDIAILKMSSIRQGGTI
                     EYEGDLKVTAAKPLNGLLMRVEFLDADGVLLSAQKIEIEEGTLSPGAERHISVQGHDV
                     PRAVSFRISFTDRAKRDLATGGAGPYPLD"
     gene            complement(16797..18878)
                     /locus_tag="VFA54_03525"
     CDS             complement(16797..18878)
                     /locus_tag="VFA54_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS49904.1"
                     /translation="MRRIGFLLSLIAFAALSQIRFETTTDLVVVDVNVRDRDGRPVEG
                     LKKEDFTIFEDGKPQQIAVFEFQRLTLEPLERAGEARSPARPSAQVAPAAMPARPALG
                     APLPRYQDRRLLVFFFDLSAMSAADQIRAQEAALKFLNQNMTRADLVSIMTFSSRLQV
                     IQDFTDDRDRLTEVIRGLRVGAFSDLADLADVGNDDAEQDTGAAFVADSTEFNIFNTD
                     RKLSALESAVKKLSALPEKKALVYFSSGVGKTGVENQSQLRATVNAAIRSNVSFYPVD
                     ARGLAALPPGGDASKAAPRGATGLFTGATQQSQRNQFNDSQETLYTLAADTGGKALLD
                     NNDLSLGIVQAQQDVRSYYILGYYSTNTAHDGRYRRIRVTLAGNPQAKLDYRTGYFAS
                     KSFAQFTSADKERQLEEALLLGDPITDLPLALEVDYFRLGTERYFVPVAVKIPGSEIS
                     LARKGANEETVFDFVGQVRDARDRLVGTVRDSIRVRLGESSAAQLGRRHLEYDTGFTL
                     PPGRYKIKFLAREDQTGKMGTFETGFVIPDLSAQTNTLRVSSVVWSSQREPLEAAVGS
                     AEKKNKLLANHPLVHDGQKLVPSITRVFRKDQNLYVYFEVYDPALDPTQKTPEVAASL
                     SFFRGSVKAFESTPVQVTEVSANRRQVVPLQFQIPLASLTPGRYICQVNVVDELGRKF
                     AFPRAPLVLLP"
     gene            complement(18882..>20356)
                     /locus_tag="VFA54_03530"
     CDS             complement(18882..>20356)
                     /locus_tag="VFA54_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49905.1"
                     /translation="LLGLPQSSSVRFGDSNTYFRGAVYNTYVSDDWRVRSNLTLNFGV
                     RYEYFTPYTEKYGRIANLDIAPNFTGVAVVTPGGAGPYTGRFPDGLIDAAKNNWSPRV
                     GIAWRPFKQRQLLIRTGYGIFYNGSIYSQFASRLASQPPFANTATLNTSLAAVLTLEN
                     GFPAAPSTQITNTYAVARNYQPGYAQTWNFSIQQELPHAIIVELGYLGTKGTHLDIQR
                     LPNRAAPGSPLTAEQRRQIGNAVGFLYDSSEGNSIYNALQARMIRRFRGGISMNALYT
                     YSKSIDNASTFGGAGALVAQNDKDLAAERGLSSFDQRHRLNLNFVLSSPVGPNARFLR
                     GGGTAAKLLQDWTLSGGITAASGTPLTARVLGNQADTGGTGSFGSGRAEATGEPVNAD
                     GGYFNLAAFTLPPPGQFGNAGRNTIPGPSQYSLNLAIGRTIQLGAETRRRLELRLESQ
                     NVTNHVNITNLGTVVNASNYGLPTAAGSMRSLNAVMRFRF"
BASE COUNT         3843 a         6135 c         6302 g         4076 t
ORIGIN      
        1 ggctggatcg cgcctgggcc gggcaaatcg aaattaacgt tgggcgcgat ataatccttt
       61 cgggtcagcg cgccgacgta gccgatgttc cagactgttc cggagacgat ctcgcgctgg
      121 accatcagat tgaactgctc cacataaggg ttgcggagat cgagcgccat cgccgtcaag
      181 tttccggtcg ggttcaacgg gtccgtcggc gctggcagcg gaaagccgtc cgagatcctg
      241 ttgatcggcg ttaatggagt ggtgctgacc gaatacaggc tcacaaacgg cggattgcgc
      301 aaggcggagg ttgagccgaa gaagatcggg tagtagttga tcccgaagcc gcctcgcacc
      361 acggtccgcg gcgtgaccgt tgccgcaaaa ccaaagcgcg gccccaagtt gttgtaatcg
      421 ccggggacgc cagcgctgcc cgacactcca ttctggccgg cgatgatgat cttgccggcc
      481 gccagatcca cgttcgagat ccggttggcc acctcgtgct gcggggtaaa aatctcgtag
      541 cgcaggccga gattcagagt cagccactgc gtcacgcgcc agtcatcctg gacgtaaacg
      601 gcaaattcgg tgttgcggaa tcccgggacg accagatact tggagcggac tgtcgccgag
      661 ggatacccca gcaaaagcga agcgatcgag ttcccgctgc gcgccacgtc gcccgaaggg
      721 tcgttcgtaa agttgccgtc gaagctgaac tggccgcgcg cctgggggct ttggaaggga
      781 ctggtttgcc gccggcgaat atccgttccc gtcttgatat tgtggcccga gcggttgtag
      841 gtcacgcttc ccacatattg gaagacgttg ttgatcgtaa tgattggaat gaaattgctg
      901 tcgcccagcc ctcgaaaccc ggcaatgacc atctgtgtca ggcccgagct gtcggcgtcc
      961 acgtttccgc cgggaatgcc cagctgctgg tcgacgttgt ttccgtaatt cggcggaagg
     1021 gtgtggattg cgtagcgcgc ataaccggcc ttgaattccg ccagcaggtt gggacgaaag
     1081 gtgtgcacgt aattcaagtg gaggccctga gcgcgctggc ggctggcccc ggcgaactgg
     1141 gcggtgtcgc cgccggcttg tatgttgccg atcttgggca gttgcggagg aagaaacgtg
     1201 ttcgtgtcgt taaacgaata ccgggcgaaa aagatgtcgc gatcggagaa atgctgatcg
     1261 attttcgcgt caaacgtgtc atcgcgctgg gttttggcag gattctgggt gaaattgttc
     1321 accaatccgc cggtctgcgg aatcggataa agggccacca cctttgccgc gaccggatcg
     1381 atcaagcttg ggggaatctg gtcgttgggg aagcggtcgc ggatcgacac cccgggtcgc
     1441 gacggatcgc tccgggtggt ggtgggatca aaaatagggt tcactccggc aaagttcccc
     1501 tgccgcatgg ccagagtggg aaccgtgctg gtgaacgttt gtccctggcg cagccggaag
     1561 ccctcgtaat cgccgaaaaa gaaggtcttg ttctttttga tgggaccacc cagactgcct
     1621 ccgaactggt tttgcttgta agccggagtc gggcccgggc ccgcaaagaa gttcttggcg
     1681 tccagatgct cgttccggaa gaactcgaag agcgagccgt gcagatcgtt ggtccccgat
     1741 ttcgtaatca gattaatgac gccgccggcg gtgcggccga tttcggcgga gtaaagattg
     1801 gtctccacgc ggaattcggc cagcgcatcc atcgatggct tgacgatgat cgttccgata
     1861 aagcgttcgt tgtcgtccat gccgtcgatg aggaaattgt tgttggtgtc gggctggccg
     1921 tttacggcga ccgaggaagc ccgccggcgg tcatcggggc gattcccgct cgacagcgca
     1981 ttgttcacgc cttccgttgc acccgcggca agacgggcca aggtgacgaa gttccgcccg
     2041 ttcaggggca gatcttcgac agcccggtca ttcaccagcg ttcccacgga agatttttcc
     2101 gattgcagcg ccggcgtttg cgcggtcact tcgacgctct gctccaactg gcctacctcg
     2161 agccgcacat ccacgcgcag ccggtcgcca accgccaaaa cgacccgggg cactgtgaac
     2221 gtcttgaagc cctgcatctc ggccctgacg ctgtaaggac ccggaggaag caaaggaatc
     2281 agataatttc ccgtgctgtc gctcaccgcc gcccgggtgg cgctggtgtc gaggttttcg
     2341 gcagtcaccc gaactccagg cagaaccgcg ccgctgggat cggttacggt tccgagaata
     2401 tcggcggtgg taacctgcgc ccgcagaggc aggcacaaca aacccgtcat ggcgcacaag
     2461 gctgcgagga ctcgggcgcc cacagcagaa cgtaaaaact tagcgttcat tttgaccccc
     2521 ttctccatcc cacttccgca gaacttcccc catcatgccc cgccgcaacg ttcaacgatt
     2581 tgtttttctt ttatacctcg ccggatcgtg tgtcaagctg gccggccggc ggcctcctgc
     2641 tcctttccag gccctggcga gcgcacggcg ccagatggag attttctttg cttcaggccg
     2701 ccacagtttg gcctcagaaa cacgcggagc aaggggccgc cactgagctc ggccggcgga
     2761 ctctggctgc cttgcggcgg gagatctcct gcccctcgag aaattcgcat cggcagcacc
     2821 gtaatctggt ctgcgagaaa taaataagcc tcgaaataga gcctacggac gcttctcggt
     2881 gtgggagttc ctggttcggg caggaagcag gctctgcggc agagcaaatg ccacgtaggt
     2941 cccgccggat ggcgcgccgg atttgccgcc gccgcaggct attatgacga actcccggcc
     3001 gccggcctca taaactgccg gcgtggcatt gccggctgcg ggcagtgtag tttcccaaag
     3061 cagagctccc gttttcttat cgaatgcgcg gaactttttg tcaaaatttg tcgcgccaat
     3121 gaagaccaag ccgccggcgg taacaacagg tccgccgtag ttctcgctgc cggtattgcg
     3181 cattcccttt tcagccaggg ccgggtactc gccgagcggg acctgccagg cgatcttgcc
     3241 cgtgttcaaa tcaatcgcgt tgagcgttcc ccatggaggc tccacggccg gatagccttc
     3301 cgggtcgaga aaccggttgt agccgtcgat gccgtatttc agatcgatcg gcgagggctc
     3361 ggtggaattt tgcacctgcc agtcttcccc gtagagcaag tagcgcgcca gagcgcgaac
     3421 cgccggctgc gggagattag caaacgcggg catccggccg gccccctgcc ggatgatcgc
     3481 gtagacatcg gcttcattga accggtcgct gagatgctcg agcgaaggaa actcgggcgg
     3541 ggtcccgtgc agatcgtcgc ggtggcagga ggcgcagttt ctttgataca gctcgcggcc
     3601 gctggtttgc gcggcgcccc ggggacgctc caccagtctc aggatccaag ccatttcgtt
     3661 ggagttcaca tagagaatgc cggtttccgg gtcaaacgcc gagccgcccc attccgcgcc
     3721 tccatcaaaa ccggggaata tgatggttcc ttcgcggctc ggaggcacga actgcccggc
     3781 gctgcgcagc ttccggaatc gctccagcac ggcctggtga gcttctggtg tgcgccgggt
     3841 gagcatggct tccgtcagca tctgccgggc gaaggggggc ggcacggtgg ggagaggctg
     3901 catggcggcg gctgcttcgc catccacgtc gctggcagga tattttctca actcgatggg
     3961 gaataaaggc ttgccggtct ccctctcgaa gagaaatacg tggccggatt tcgtggtttg
     4021 ggcgaccgca tcgaccagac ggcctccgcg ccggacggtg accaacgacg gaggcgacgg
     4081 aaagtcgcga tcccacaggt cgtggtgcac ggactgaaaa tgccagagcc tcttgcccgt
     4141 ccgcgcgtcg agggcgagca ggcagttggc gaacaagttg tccccgaggc ggttggcgcc
     4201 gtagaaatcg aatgcggcgg atcctgtagg agcgaagacg atcccgcgct tcacgtccag
     4261 gctcattcct gcccaattgt tcgcgccccc gatgtattgc caggcgtcct tcggccaagt
     4321 ctcatagccg aactcgccgg gatgtggaat cgtgtggaac gaccagcgca acgcgcctgt
     4381 ccggacgtcg tacgcgcgga tgtcgccggg agaggaggga agcgtttcgc tcacgatgct
     4441 gccgatgatc agcaaatctt gatagacgat gcccgggctg gtggccgaaa tcgacatctg
     4501 ctggggattg cgccccaggt tctcgcgcag atcgatgcgg ccctcgcggc cgaaactgga
     4561 aatgagacgg ccgttgcggg cgtcaagggc ataaagatat tgccgggcag caaaaaagat
     4621 gcgctcgtca acaccgtcag accaatacgt gacgccccgg ttgcgggctt tgcccaacac
     4681 ctttcggccc tggctgggat cgaagctcca gcgcagtttc ccacttgccg cgtcgagggc
     4741 gatgacgcgc agcttgggcg ttgtagcgta caagactccg tttacaatga tcggattgca
     4801 ctgcatctca gagccggaaa actcatcgcc ggtatcgaag cgccaggcca tttccagccg
     4861 gcgcacgttg ctgcggtcaa tttgtttcag gctggagtag cggatgcttt cgggcccgcc
     4921 gccatagacg ggccattcgc gctgcgcctg cctcgcgatc ggctggctgg cgaacccgta
     4981 agcggccaac agatggaatg cggccaccaa gaccgcgcgg aagtttggca tcagcgttcc
     5041 tgatgtcaga ttatcattgg cgattcgggc cccggtcaat agctcgcggc tgcggcggca
     5101 gattgcgcaa gaatggctgc actgccttgc tgctgctgct cttctcgtgg aaaatgaaga
     5161 ctccgatggg cgcccgccgc tacagatgag atttcggatc gcattcgcgc tcctttgcgc
     5221 gcctttcttc tgtgcgcggg gtctttttgc cgccgagccg gagcccccgc ggcagcccat
     5281 tccgtacagc cataagaccc acgccgccgc cggcttgcag tgccgggatt gccacgagat
     5341 gcccggggca gggaaggccg ccggcaaccc gaaggaagaa aggtgcatgg ggtgtcatgc
     5401 caccgtaaag cgcgagagtc cggccattca gaagctcgcg caatattaca aggaccgaaa
     5461 acctgtgccc tgggcccgcg tttaccagat tcccgattac gttttcttct cccacaaggc
     5521 gcacctgagc cggcaaggca tttcctgcga gacctgccac ggcccagtcg cccagcgcga
     5581 cgtaatcacg atggagaagc cggtcaatat ggccgactgc atgttctgcc acgaccagtg
     5641 gcgcgtcccc attgcgtgca acgcgtgcca cgagtaaaag ccggcggcct cgggcacgga
     5701 tggctgagtc acctgtcaag atcggatacg ggcgtgggcg cgcctgttcg aaaccttttc
     5761 cgcaagccgt gtcataatac ctccatgcga cttccgaaat tactgcgcgt gaagcagcac
     5821 ttcccgagtc gcgtccttgc cgacgtgggc ggggcggtgc ggaaggaatt ggaatcagcg
     5881 gcgtggacct ggcaggtcaa gccgggctcg cgcatcgcca tcggcgtagg cagtaggggc
     5941 attacgaata tcgcaatgat cgtcaaagcg gtggtcgatt tctggaagtc gcgcggcatg
     6001 cgcccgttta tcatcccggt aatgggcagc cacggggcgg ccacggccga aggccaggcc
     6061 gatgtgctgg cgcactacgg catcatcgag cagacgatgg gcgcgccgat catcagctcc
     6121 ctcgatgtgg tgccgctcgg cccgacgccc gaaggcatcg aagtggtcat ggaccgcacg
     6181 gccttcgaga gcgacggcgt gatgctggtg agccgcatca agtggcacac ggatttcgcg
     6241 ggcaagctcg agagcggcgt tcacaaaatg atggccatcg ggcttggaaa atacgccggc
     6301 gcgcagcact accacacctt tgccgtccgg ctcggccacg aacgagtgat tcgcagcgtg
     6361 gggcgcaagg tgctcgccac cggcaagatg ctgggaggac ttgccattct ggaggacgcc
     6421 taccacaaca ccagcgaggt gcacgcgctc ggcgtgaagg gcatggagcg gcgcgaggag
     6481 gaactgctgg agcgtgtgaa atcctgggcg ccgcgcattc cggcgccgga aatcgatttg
     6541 ctgattgtgg acgagatggg caagaacatc agcggctcgg gcatggatac gaaaatcgtc
     6601 aatcgctcgg tgacgggcga caacaacgtc tggcccaacg cgccgaagat catgcgcatc
     6661 tttgtccggg atttgactgc gctgagctat ggcaacgccg tgggcctcgg catggcggac
     6721 gtgatcaccg ataagctcta caacaaaatc gatcgcgttc ctaccgagct caacgctttg
     6781 acggcctcca ccacgccgat ggccagaaca ccgatccact atccgacgga ccggatgtgt
     6841 ctggagaaag ttgtccccac gctcgggcgc attgacacca tggaagcgac gatcgcctgg
     6901 attcgcaaca cattggaact cggcgagctg atgctcagcg aaaatctgcg cgacgagatc
     6961 cgccgcaatt ccatgctgga gattgttggc gggccggcgc cgatcgagtt ccatccggat
     7021 ggcagtctgg tggaactgct aggcgcgcca cagcatgcgc cggaagcgct tacccaccgg
     7081 tagcgactgg aaatcctgtc atgccactgt atcaggcggt tgtcctggcg attgtccagg
     7141 gcatcacgga atttctgccc attagcagca ccgcccatct ggctctggtt ccctggctga
     7201 tggggtggcg tgacccgggc ctgacgtttg acgtcgcgct ccatttggga actctggcgg
     7261 ccgtgctggt gtatttcgtg cgaacgtgga ttcgcctgct ctcgcaggcc ctcggacgcc
     7321 gcggcgaccc aaaagacgac ttctccgcca accctcgttt gttctggttc ctggtcgtgg
     7381 ccacgatacc cggcggggtc gcaggcctcg ccctcgaacg ctacgccgaa agcactctcc
     7441 gcagcccata cgtaatcgca gccacgatga tcggcgtcgg cctgctgctg tggtggtcgg
     7501 aaatgaaggg cagacttcaa aaggatttgg atgctgtgag cctggccgat gccgccgcga
     7561 tcgggactgc ccaggcgttc gccgtcgtgc cgggtgtttc ccgctcgggt attaccatcg
     7621 cggcagggct gttccgcaac ctggaacgcg ccgcagccgc gcaattctca ttcctcctgg
     7681 ccacgccgat catcgggggc gcagctctca aagccgcctg gaacatccac cgcagcggcg
     7741 ggcttgcccc cggcatgcgc acgcctttcg ccctggggat actggtaagc gcggtcaccg
     7801 gatatgcagc tatcgctttt ctcctccgct acttgcaggc aaggacactg aagtttttcg
     7861 tctggtatcg cgtcatctgt ggcataatga tactcgctct ggctttattc ttccgcgact
     7921 ccgtcatgca tccgtgagcc gtccgccagc ggcagcggtg aagggacgga ggccaacgaa
     7981 ggatgagatt tctatctccg accagccgcc gcagggtcaa cgagctggtc ggcctgttgc
     8041 tgttgttgtc aggcatcgcg ttggggctga gtctggagtc ctacagcccg ctcgatcctt
     8101 cctggaacac cgcttcgccg gcctcccggc ccgaaaatct tctcggctac ccgggctctt
     8161 acaccgctga cctcgccttc cagctcctgg gtcttggcgc gtttctgctg cctttgttga
     8221 tgctagcgct gagctggaaa tggatccgat cagaaccgct ggacgtttcc aaattgatcg
     8281 gcgcattttt gctggtggtc aactgctgcg cggcccttac agtttttcgt cctgcatttt
     8341 tgtggaaaga caccatcgag ataggcgggc tcaccgggcg ggtgacggct gacctgctgc
     8401 tctctaatct gaacttgact ggcacgttga ttgcgatagc catcggcggc ttgctctccc
     8461 tctatctgat gactgcattc agtcttgaac gatttggcgg atggctgaag cggcggttcg
     8521 cattcctcgg caagtggcgc aagcgctacg ggcgctggcg ccaggaagtc aaggaacgga
     8581 ggcgccgcga agaggccgcg agatctgagg cgctagccag gcggcggatc gaccgcgagg
     8641 ctgaggtcaa ggacgcaagc ggctccggca gggtcgcaaa gaatgagccg ccggaggcgc
     8701 cggtggttga actgccggcc ccagtagcag agccggcggc cgcgtttgcc gaggatccgc
     8761 cgattcgcga gctggaacct gcccctgcgc cggagcggca tcgggcgccg aggcgcacgg
     8821 ggccggcaag gatcgccgca gcgaacttca aaatccctcc cacctcgctc ctcgagccgc
     8881 cagcgggccg caatccctat gacgaagagg aactgcgcca actcgccgtc atgatccgct
     8941 cgaagttcga ggaattcaat gtgcacggcg cagtcaccca gatcaatccc gggccggtcg
     9001 ttaccacttt cgagttcaag ccggaagcgg gagtcaaata cagccgcatc acgaatctgg
     9061 cggaggatct gtgcctgggc ttgcaggcgg aatcgatatt gatcgaacgg attccgggca
     9121 aatctaccgt gggcatcgaa gttcccaacc ggcgccggga ggtgatcagc ctgcgagaaa
     9181 tcatcgaatc cccggaattt caggactcgg gctcgcacct gacattttcg ctcggcaaag
     9241 atatcaacgg ccggattcgc accgccaata tcgaatccat gccgcatctg ctcattgcgg
     9301 gatcgacggg ctcgggcaag agcgtgctga tgaactgtct catcatgtcg atcctttaca
     9361 aggcgacgcc cgacgaagtg cgcttcatca tggtcgaccc gaagcggctg gagatggggc
     9421 tttacgagga tattccgcat ctgctcacgc ccgtgatcac tgacttgaag aaggcctcgt
     9481 acgcgttgcg caacgccact atcgagatgg agcggcgctt gaagctgctt gctacgcaag
     9541 gtgtgcgcaa catcgaccaa tacaacaaaa aggtgcgcgc gcaggcgtac aaggagttgt
     9601 cgctcttcga agaagacgaa gcggccgagc ctgccgagcc gctgccttac atcctgatcc
     9661 taatcgacga gctagccgat ctgatgctgc tcgaaggcaa caatgtcgaa gagtcgatta
     9721 cccggctggc gcagatggcc cgggccgttg gaattcatct ggttctggcc acgcagcggc
     9781 cgagcgtgga tgtgatcact ggcttgatca aagcgaattt tccggcgcgt atctcgttcc
     9841 gggtcgccac gcgagtcgat tcccggacga ttctcgattc catgggggcc gaggcccttt
     9901 tgggccgcgg cgacatgctg ttcctgccgc cgggctcgag ccgcatgatc cgcgtgcatg
     9961 ggccgtacgt gagcgaatcg gagattacgg aagttgtcaa gttctggaag gagcaatcca
    10021 aggcggcgta cgaaacttcc tacctcgcgc cgccgccgaa ggaagaagac ggcaaggaga
    10081 gcgaggacga agccgaattc gccggcgaag aggatccgat gtacgaggac gctgtgcgcc
    10141 tcgtgctcga aatgggcaag gcctcgacct cgattctcca gcgccggctt cgcctgggtt
    10201 atgggcgcgc ggcgcgcatt ctcgatgtca tggagcgcga cggcatcatc ggcccgcccg
    10261 acggcagccg cccgcgcgag gttttgaagc gccccgactg gctgctggaa gtagataatt
    10321 cgctgcggta ggcgtgcatc cgcgcctact gcaagctcgc ggccagagct ttatcgagct
    10381 ctggcgtgat ctgattgagg ctttgcgggt tgttcatgag gtttcgcaac gccatatcgc
    10441 cgtgctgctg cacgaataat tccgtcgcgg cgagcgccag agtataggca atagccgcct
    10501 gcttggtatt caaccccgac cagctatttc cgagatcttc cagcttcggc agttgtccta
    10561 cccgggccag ggttttcagc atgtcccgct ggacggggcg cagcgcgtcc cccgagagct
    10621 tttgagcaac gccttcgtgc agccaggtcg gccagcggcc gatattcgcc atacaggcgt
    10681 gcacgatttc gtgggcgaag gtctgccggg tttcggggtc aatcgtggcc gtgcgggcca
    10741 gaggaatacg aatctttccg tcgtaaacgc cgccgctcca ctccgccgct ccggtggtct
    10801 gccggtacgc ctcgcggctt tgcacgatcg taataatgcg ctcgtcggcg ggacacccga
    10861 gctggaacga gattcgggaa aactcctgct cgagcacatc gaccatgctg cgggccaact
    10921 ccggactggc cacggcattg tcgtagcgga gcaaaaagcg catgccatag ctcttttcgc
    10981 cgctcttatc cgcgctcatc tcgcgctcga tgcgggcata aattttctgg agcgtctcat
    11041 cgggcttcag ttccaaggat tctttcagat aggccagcgc gcggggaagg tcatcgaggt
    11101 tgtaagctgc gcggcccgct gcggcaaaaa gctgcggatc ctttttattt gtacgcaaag
    11161 ccggttcgag cacatccaga gctcggtccg gctgattcaa ttgcagcagc gcataggcgc
    11221 gcaggagcgc caccgcgcgc tggccgggcg gcgcgggagc cttttccaga atccgctccg
    11281 catcggcagg ccggcccgat tgtagagcat ccgccgccag gttcagttgc gccgccaaag
    11341 catagttggt ctttccggtt tcctgctcct ctaccgcctt ctcgcgggcg gattccacct
    11401 cgctccgtga gatcacaggc agagctcttg cagcggccgc cgagtcgctg cggcgcttcg
    11461 cctcatacgc ttcgagattc gtcagcccgt cggccgggac atagccttgc agcgtcttgc
    11521 cgccggtctc gaccgacacg gcatagcacg gctttgcatt gccggtcagc gagaagcgga
    11581 tccggaccgg gtcgccgcgt ttgagcagcg ccacggtctc cgccgcgtcc gagcacgagg
    11641 cgcgcagtgg gtaattgtcc tccttgatcg tctgggctgc gctcgcagag gccaaaaaca
    11701 gcgccgtcag gcaaggcaga aacagtaggg cggctctcat ggcattgacg atagcacgcg
    11761 ctacggccgg tcctgttaag gcaaaaccct aatttctaga aaccttcttc ccgcaggcgg
    11821 tcagccgtga gccggcggcc aggcttcaga tccatcgcct ggagcagctt ctctacgtgc
    11881 ttggcgatga tgtcgcattc caggttgagg cggtcgccgg gacggcggtg cgaaaggttc
    11941 gtcgcgctat aagtgtgcgg aatgatcgcg acactcaact cgccgccttc cagggcggca
    12001 atggtcaggc tgatgccatc tatggcgatg ctgcccttga atacgaggta tcgctcgaga
    12061 tcgcggggcg cgcggacctt taaccaccag tttccatccc gcagcgattc gaggctgacc
    12121 agttcgccgg ttccatcgac gtgcccctgc acgatatggc cgctgatccg cccgttgggg
    12181 gaaagcggcc gctccagatt tacgggatcg ccccggcgca gatcgccgag gttcgtgcgg
    12241 gcgagcgttt ccggcgaaag gtcggcgaaa aaaattggtc cttttacctg gacggccgtg
    12301 agacaaacgc catttacact aatgctggcg cctgcggtca gatccgagca cacgcggtcg
    12361 gcgcgaacgg ccaatttgct ccccggtgtc tctacaatgc ctgtttcttc aatgattccc
    12421 gtgaacatgc agttagtggc cggcggttgg ccagtgtatc cgcggccggc ctcggccgca
    12481 gaaatcggca atcgactgta tccgtcacgt ggacatcgcc cattctacga tggcaaagcc
    12541 atctgccagc tctatgcgga aaccggagcg caaccggcgc caacggcggc cgctcctgat
    12601 tggttccgcg ggcgcacccg catattcgtt gccacctaaa gcaaccctgc cgccgctgaa
    12661 agctgaagaa gcggttggct gcgttgccgg gagccgatgc cggaaggcta gtccgcgcgc
    12721 agcgtgtcca gtatctgata gaactccgga aacgaaaccg aagccgcctc cgcccggtga
    12781 atcactgagg gcccgtccgc agctaacgcg gcgacggcaa aggccatggc gatgcggtga
    12841 tccccgaagg aatcgatctc ggcggcgcgg aaacgggact tgccgggaat ttccagtccg
    12901 tcctgccatg ccctcgcctt cacgcccatg cgtcccaagt tgtctgccac ggtggcaata
    12961 cggtcgctct ccttcacccg tagctcggcg gcattttcaa tcaccagccc gttttccgat
    13021 gacgcgccca gcacggctag caccggaatc tcatcgatta cggcggccgt cgtcgctcct
    13081 tggataaccc ctccccggat cggcgaactg cgcacggcga cgtctccgac cggctcgcca
    13141 tgcacgtgcc ggacctgcga gattgccagt tctgcgccca tcgatagcag aacatcgagc
    13201 agagcggaac gggtacggtt gaggcccacg cccttgatta caacatccga gccaggtacg
    13261 agcagcgctg ccaccaggaa aaacgccgct gaagagaggt cgctcggcac aaaaaagctg
    13321 ccggcgcgta aggccggccg cggctccagg cgaatgcatt ggccctgcac ctggatagcg
    13381 gctccaaatt cgcgcagcgc gatctcggta tggtcccggg ttgaaacggg ctcacggacc
    13441 gtcgtagggc cccgggcata cagccccgcc agcagaattg ccgtcttcac ctgggcgctg
    13501 ggaagcggaa gctcgtagtc aatgccgcgc aagggcgcgc cctgaatcct taaaggagga
    13561 aagttcccct cccatgcttc gattttcgcg cccattttgg tgagggggtc gaggatgcgc
    13621 cccatcggcc ggcgcttcag cgatgcatcg cccgaaattt cgctctggaa cggctgggcg
    13681 gccaaaatgc ccgagagcat tcggatcgtc gagccagaat tgcccgcatc cagaggccgc
    13741 gccgccgctt gcaatccact cagccctacg ccagtgatcg aaactgtggc cttctcggaa
    13801 gcgatcggca ctcccaattc gcgcaggcag gcaagtgtgg aagcgcaatc cgcaccggta
    13861 gaaaagtttt ccaggcggct gacgccttcg gcgattgccg ccagcatggc ataacgatgg
    13921 gagatggatt tatcgcccgg aagccgcacg acaccggcca gacgctgcgc gggaaaaata
    13981 atcttgtcca ttcaggaagg gacctgcggg tccaagtacc aattctagca gtccgcctga
    14041 aaagcttatg gagcggcaaa gcttcggtct gtgcgttggt cggtggtggt ccgctacggg
    14101 gtgcgccgcc agcgccgcag aataatcatc agtagaaatg ctacggcgaa gaccaggcag
    14161 acgagccgaa aatcatccgg agtcatgggg taagcggcgg gcttcgaact gcgccgctac
    14221 gaagcttatc ggcaccctgt cggctggcct tctacttttt tgccgggccc acgcctgagc
    14281 ggcgtttctt acgtcgtttt tccttgatct ccccggcgat cttgggaggc cgcccgcggc
    14341 ggggcttccc tcgggccagt gcttcgagcg accacaacgc gtagctgatt tcttgcagct
    14401 ccctccgtag ctgctcgatc attttgcgca agacagcatt cttcacgtgc gttctccggg
    14461 catccagtag tccatcggag ggagcaggca tacggtttag acccgaatcc cgcagtttta
    14521 cgaaactttt acaaatctca tacgactctc tgctgggagg cccctaatat cgagacagaa
    14581 atagaaagca ggatcaaatt catatgtttc tcaaagaaga aaagaccacg agagacgcca
    14641 cttatcagga tcctcatctc atcattcact ttcagcggca gagagtggaa ctggatggcg
    14701 cgactctaac attgacgcgc aaggaatacg atctgctggc catgttgtcc caaaacgccg
    14761 gcgagattgt tcccagggat gtgctgctga ggcaggtttg gggatactgc gaagggattc
    14821 gaacccgcac gctcgatgtt cacattcgcc ggcttagaaa aaagctggag ccattctcgg
    14881 ataggtacat agagaccatt ttcggcatcg gctatcgctt ccagccgttc cggccgcgga
    14941 cggccccata cgagatgcaa aagcgcatgg cagtatccgc tatcgcctga ggcgcagcag
    15001 gccgtttcgc ggcgagcagg tctcgcaggt ccggcctctt tttgtctata ctcgttcttg
    15061 cactcgctca ccccgaatca aatccgaggt ttttgggcgg cttggggcgg ttgggcgctg
    15121 gatggaatgg attccttcat ctacgccctg gttatggttc ctgcgctccg ccaactgctg
    15181 cctcggtcgc acattccgcc gagcgcggcc aatatcggct tttacggggg cctgctcttc
    15241 gcgctgtttc tggtgggctg gggttttgcc tttgtgtggg gacccatcgc tgatcgcttc
    15301 ggacgggtcc ctaccttgat gcttaccatc ctctgctatt cgctgtttac ttttctcggg
    15361 gctggcgccg ccaacgtctg gcaattggcc gcttatcggc tgctggccgg actcggtatc
    15421 ggcggcgaat gggctatggg cggcactttc gtcgcggaag agtggcccga ggaccggcgc
    15481 aaaatggctt ccgcttacat gcacaccggc tactacttcg gtgtgctcct ggccggcgca
    15541 gccaactcga tcatcggtgc gaattacggc tggcgggcca tgtttgcgct gggcggcacc
    15601 ccggccctgc tcgtcgcgtt catccggtat ggcgtgaacg agccgcagcg gtgggaagtg
    15661 caggaaaagc catccatgcg aaaggcgttc tttgcccttt tctctcccga gtaccgccgc
    15721 cggacgctgc tcaattccgt gtatcttttg gtatcgattg tgggactgtg ggccggctca
    15781 gtctatgtgc cggcggcggt cgcccaagtg gccgcccggg aaggcttcag cccaaggcaa
    15841 ggtacccaat ttgcttccta tggaacgatc ctcctctcgc tgggcacgat tctcggatgc
    15901 ctgattctgc cggcattggc ggagaagctt ggcaggcgcg ccgccctcgc gttctacttc
    15961 gcgctcatgc tcattttcat tcccgtttcg tttgggtacg ttttttactt gcgccaagga
    16021 gcgctgctgt ggttcctggt ttgcctgttt tttcttggtg tggggggagc caatttcggt
    16081 atgtacacgc tatggctgcc cgaacagtac cgcaccgaat gccgggcaag cgccttcgcc
    16141 ttctccacct cggtagggcg ttttatcggc gccgggatta ccttcctcgt gggcgccgga
    16201 gtttctcatt tccacacgct gggaatcccg gttgctctga ccggcgcggc cttcgcggcg
    16261 ggaattttgc tgttgccttt gggcgttgaa accaggggca agccgctgcc ggcctgatct
    16321 ccgtttagtc cagcggatac ggaccggcgc cgccagtggc gagatcccgc ttggcgcgat
    16381 cggtaaatga gatccgaaag ctcactgccc ggggcacgtc gtggccctgc accgagatgt
    16441 gcctttctgc gccggggctg agcgtgcctt cttcgatttc gatcttctgg gcgctcaaca
    16501 gcacgccgtc ggcgtccagg aattcgacgc gcatgagaag gccgttgagc ggcttggccg
    16561 ccgtgacctt cagatcgcct tcgtactcga ttgttcctcc ttgccggatg gaggacatct
    16621 tcaagattgc gatatccggt tcgcgcttcg gtttttggcc cggccgcgcc tgcccggaca
    16681 gggcggccaa gcagacggct agaaataacc ttcgcgtcat agagacaacg tagcgtcaag
    16741 tttagccgga tgctttccgg cccgcaaggg atgaaggttg gaaggccgcc aatgcctcag
    16801 ggcagcagca ccagcggagc gcgggggaac gcaaacttgc gtcccagttc gtccactaca
    16861 ttcacctggc agatatagcg gcccggagtc aacgatgcca gcggaatctg aaactgtagc
    16921 ggtaccactt gcctacggtt tgccgatacc tctgtcacct ggactggagt cgattcaaaa
    16981 gccttcaccg atccccggaa gaagctcaga ctggcggcta cctccggcgt cttttgcgtg
    17041 ggatcgagcg ccggatcgta aacttcaaaa tagacgtata ggttctgatc tttgcgaaaa
    17101 actctggtaa tgctgggaac gagcttttgg ccgtcgtgca ccagcggatg atttgccagc
    17161 agcttgtttt tcttctccgc cgagccaacc gcggcctcaa gcggctcccg ctggctgctc
    17221 cagacgacgg aactgacgcg gagggtgttg gtctgcgccg acaaatccgg tatcacgaag
    17281 cccgtttcga aggtgcccat cttgcccgtt tgatcttcgc gggccaaaaa cttgattttg
    17341 tagcgtcccg gaggaagcgt gaagccggta tcgtactcca ggtggcgtcg gcctaactgg
    17401 gcggcgctgg actcgcccag cctgacccgg atgctgtccc gaactgttcc caccagcctg
    17461 tctcgggcat cccgcacctg gccgacaaaa tcgaaaacgg tctcctcatt ggcgcccttg
    17521 cgcgccaggg agatttccga ccccggtatc ttgacggcga cgggaacaaa atagcgctcc
    17581 gtgccgagcc ggaaataatc cacttccagc gccaaaggca aatcagtgat cgggtcgccg
    17641 aggagcagcg cttcctccaa ctgccgctcc ttatcggcgg atgtgaattg cgcgaagctc
    17701 ttggaagcga aatatccggt gcggtaatcg agcttagcct gcgggttccc cgccagcgtg
    17761 acgcggatcc gccggtatcg cccgtcgtgc gcggtattgg tgctgtaata gcccaaaatg
    17821 taatagctgc gaacatcctg ctgcgcctgt acgatgccca gcgacaggtc gttattatcg
    17881 agcagcgcct tgcctccggt atcggctgcc agcgtgtaca gcgtctcctg cgaatcgttg
    17941 aactggttgc gctggctctg ctgggtcgcg ccggtgaaca gcccggtggc gcctcgggga
    18001 gcggccttgg aggcgtcgcc tcccggcggc aaagccgcca ggccgcgggc gtctaccgga
    18061 tagaaggaaa cgttcgatcg gatggccgca ttgacggtgg cgcgcagctg cgactggttc
    18121 tccactccgg tcttgcccac gccgctcgag aaatagacga gagccttttt ctccgggagg
    18181 gccgaaagtt tcttgacggc ggattcgagg gcactcagct tgcgatcggt attaaaaata
    18241 ttgaactcgg tcgaatcggc cacaaacgcc gcgcccgtgt cttgctcggc gtcgtcattg
    18301 cccacatcgg ccaggtccgc caaatcggaa aaagctccta cgcgcagccc gcgtatgact
    18361 tcagtcaggc ggtcgcgatc gtccgtgaaa tcctgaatca cctggaggcg gctggaaaac
    18421 gtcatgatgg aaaccaggtc cgcccgggtc atgttttggt tcaggaattt cagggcggct
    18481 tcctgagcgc gaatctggtc cgcggcgctc atcgccgaga gatcaaagaa gaaaaccaaa
    18541 agccggcggt cctggtagcg cggcaggggc gcccccagag cgggacgcgc cggcatggcg
    18601 gctggcgcaa cttgtgcgga aggacgggcg ggcgaacgcg cttcgccagc gcgctccagc
    18661 ggctccagcg tgaggcgctg gaattcaaaa accgcgatct gctgcggttt gccgtcctcg
    18721 aagattgtga aatcctcttt cttcaagccc tccacaggac ggccgtcgcg gtcgcgtaca
    18781 ttgacgtcca cgaccaccag gtccgttgtg gtctcaaagc ggatttgaga aagcgccgca
    18841 aaagcaatca gggagagcag gaatccgatc cgtctcatag ctcaaaatcg aaaccgcatt
    18901 acggcgttca ggctgcgcat cgaacccgcg gcggtgggga gcccataatt ggaagcgttg
    18961 accaccgtgc ccaggttggt gatatttacg tggttcgtga cattctggct ttccagccgc
    19021 agctccagcc ggcggcgggt ttccgctccc aactggatcg tgcgcccaat tgcgagattt
    19081 agcgagtatt ggctggggcc ggggatggtg ttgcgccctg catttccaaa ttgacccggg
    19141 gggggcagcg taaacgccgc caggttgaaa tacccgccgt ccgcattgac cggctcgccg
    19201 gttgcttcgg cccggccgct gccgaacgat cccgtgccgc cggtgtcggc ctggttgccg
    19261 agcactcgag ctgtcagcgg cgttccactg gctgccgtga tgccgccgct gagggtccaa
    19321 tcctgaagca gtttggctgc cgttccgccg ccgcggagaa accgggcgtt cgggccgacc
    19381 ggtgaagaga gcacaaaatt cagattcagc cggtgccgct ggtcgaagct ggaaaggccc
    19441 cgttccgccg ccaggtcttt gtcgttctgc gccaccaggg ctcccgcccc gccgaacgtt
    19501 gaagcattgt caatcgactt cgaatacgtg tacaaagcgt tcatcgaaat gccgccgcgg
    19561 aaccgccgga tcattcgcgc ctgaagggcg ttgtagatcg agtttccttc cgaactgtca
    19621 tagagaaagc cgacggcgtt tccaatttgc cggcgctgct cggcagtgag gggcgacccg
    19681 ggcgccgctc gattcgggag ccgctggatg tccagatgcg tacccttggt cccgagatac
    19741 cccagctcca cgatgatcgc gtgcggaagt tcctgttgaa tcgagaagtt ccacgtctgt
    19801 gcgtagcctg gctggtagtt gcgggcaacc gcataagtat tcgtgatctg tgtcgaaggc
    19861 gcggcgggga agccgttttc gagcgttagc accgcggcca ggctggtatt caaagtagct
    19921 gtgttggcaa acggcggctg cgaggccagc cgggaagcga actggctgta aatcgagccg
    19981 ttgtaaaaaa tcccgtagcc cgtgcggatc aaaagctggc gctgcttgaa aggccgccag
    20041 gcaatgccga cccgcggtga ccagttgttt ttggcggcgt cgatgaggcc atcagggaac
    20101 cggccggtgt aagggcccgc cccgcccggc gtgacgacgg ccacacccgt aaaattgggc
    20161 gcgatatcca ggttcgcgat gcgcccatat ttttcggtgt agggcgtgaa atactcatac
    20221 cttactccaa agttgagcgt caggttggag cggaccctcc agtcgtccga gacataggtg
    20281 ttgtaaacgg cgccgcggaa gtacgtattc gagtcgccga agcgcaccga actcgattgc
    20341 ggcagcccca gcaaaa
//
LOCUS       DASREK010000060         5638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1517374, whole genome shotgun sequence.
ACCESSION   DASREK010000060 DASREK010000000
VERSION     DASREK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517374"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFA54_03535"
     CDS             complement(<1..215)
                     /locus_tag="VFA54_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZS49906.1"
                     /translation="MMRDGNGMRYVLDDVKVLDFSHALAGPYCTLLLAAYGARVFKLE
                     PPGTGEIARHWGPPFTGKEASFFLGLNS"
     gene            461..2647
                     /locus_tag="VFA54_03540"
     CDS             461..2647
                     /locus_tag="VFA54_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49907.1"
                     /translation="MRRSEGYFLLGALLVLAIPAGGWGQSSSVITTIAGNGIPGYNGD
                     GIPAAQAQFNFLVSIDPQFEEYSHPAIDLEGNLYIADQGNDRIRKISPDGTITTIAGN
                     GQTGFSGNGVPATQAELNSPISVVLDQKGNLYIADQNNHRVRKVAPDGTITTIAGAGT
                     DDLSGNGGLALQAELNSPGGLAVDAAGNLYISDTYNSQVRKVTPSGVITAFAGKGGEP
                     AYFGDGGPATLAGLAFPAGLAFDAAGNLYIADQQNNCIRRVAPDGIITTVVGDGTGDF
                     GFRGDGGPAKQALLNYPSDVAFDAEGDLYIADEDNNRIRKVSPSGIITTVAGDGQAIF
                     NGDGGPAANAGLNRPSGLAFDKGGNLYVVDQYNWRIRKIVFNPPVLSVSAAKLGFAAE
                     AGGPNPAPQTFTVTNAGSGTLNFSITGNQSWLTITPASGAVSSGASVTITVNARSANL
                     NAGDYEGALVISAPGTLEGSRTVQVSLAVRAVTAPGPVFTAAGVVNAASFASGPIAPG
                     EIISIFGSNLGPAVSTGAVIDASTARLATIVAGVAVLFNNLPGALFYVQEKQINVQVP
                     YELASRTGAVPGVVTIVVSYQGASSAPVSVPAAAAAPAVFTVSRGLGQAAALNQDNSP
                     NSAANPAGRGSVVQIFMTGQGPTNPPALTGQLTQPPYAAPLQTPTVTIGGITAKTIFV
                     GLAPNLAGLLQIDAIVPVDVVPGPNVPLEISIGAASTQTGVTLAVR"
     gene            2799..3575
                     /locus_tag="VFA54_03545"
     CDS             2799..3575
                     /locus_tag="VFA54_03545"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZS49908.1"
                     /translation="MTRLQNLFDLKGRVALVTGGSRGLGREMAEGLAEAGASVMLVAR
                     REQWLKPAVAEMKAAGFTCEGMLCDVSVPDQVDAAVTRTCEVLGGIDILVNNAGVTWG
                     APIEQMPLDKWRMVLDTNLTGAFLVAQRAARVMLARKNGVIINVASVAALKGIAELGY
                     STIGYIASKAGLIAMTREMAARWGRSGIRVNAIAPGFFPSRMTEKVLERIQAEYEKRT
                     PLGRVGREGELKAVAVFLASPASSYITGQTIVVDGGASIV"
     gene            3572..>5638
                     /locus_tag="VFA54_03550"
     CDS             3572..>5638
                     /locus_tag="VFA54_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZS49909.1"
                     /translation="MSARELKDKQRKAVESWGGDVCVVAGPGSGKTFVLVERFRWLVL
                     EKNISPHRILAITFTEKAAASMRRKLIESFPAGSRHRAEVERSCISTIHGFCARLLRE
                     NAIAAEVDPEFRVLDEWEADFELRRAIDGALEQAYSAEPERARRFLAAFASDQVHESL
                     FCLYQALRAAGASISQASRIPPSCSLQQSWAALVQAFDQTAACSTAGWNTNQRDALGD
                     ALAVRKRLAELAGLPASIAHFQALRQIDFVVTAFKRGSRQRELLTQIRDELAPRCRRA
                     LLLEANAENRQWFLETLSKADSLYRAGKQQRAMLDYADLEEYAIRLLERTGGRIAATF
                     DHILIDEFQDTNALQSRLVSRLRQGARLFVVGDINQSIYGFRHADPEVFRAYREQTRS
                     GGGEVHELAENFRSRQEVLDAIKAIARDAPGIEVQELTAGTRFLTASQPCLEVLVVHA
                     EDGDRAFHLEAQHIAARIRELCGHLTLKAGEENPGGEPQTRVAGYGDFAVLCRTSMQL
                     RIIEQVLRDQGLPCQITEGRGFYDTREVNDLIHFLRALWNPLDEISLAAVLRSPLAGI
                     SDESLFRAKLAAGSLLGGLRHGLDDNAAAPHCKLPPDEAAKVKSFLTLFDRWRARRDY
                     MTIDRLLAGVLADTGYFEQLLRRPGGPQKAANVSKLMALARRFHASGQAGLDEFIERA
                     QQLRRFQ"
BASE COUNT         1075 a         1662 c         1841 g         1060 t
ORIGIN      
        1 gaattcaggc ccaaaaagaa ggaagcttct ttcccggtga acggcgggcc ccagtggcgg
       61 gcgatttcac cggtgccggg cggctccagt ttgaacacgc gagcgccgta ggccgccagc
      121 agtagagtgc aatagggacc cgccagggcg tgggagaaat cgagaacttt cacgtcgtcc
      181 aacacgtagc gcatgccgtt cccgtcccgc atcataccgt gaaaaatgcc ttgacgccgg
      241 ggctttcggg atttcgttct tccgggtatc ggatcggctt cgcgttttca aaatgcattc
      301 cgtgcgttct cgttacggcg ccccgcgcgg aaccgcggac ttttttggtc aagccgccag
      361 cattgtgcga tctgctgtcg agaaggcttg agaactcgcg aggcggcgtc gcgcccggga
      421 ccccacattt gttcgcaggt tgataaactg atccgggtat atgaggcgaa gcgaggggta
      481 tttcctgcta ggcgcactgc ttgttctggc gatcccagcg gggggctggg gtcaaagcag
      541 ctccgttata accacgatcg ccggcaatgg gattcccggg tacaacggcg acggcatccc
      601 cgccgctcag gcgcaattca atttcctggt cagcattgat ccgcagttcg aggagtacag
      661 ccatccggcc atcgacctgg agggcaacct ctatatcgcc gatcaaggca acgaccggat
      721 ccgcaagatc agtcccgacg ggacgatcac gacgattgcg ggcaacgggc agaccggctt
      781 cagtggaaac ggcgtgccgg ccacgcaggc cgagctgaat tcgcccatat cggtggttct
      841 ggatcaaaaa ggcaatctct acatcgccga ccagaacaat catcgggtgc gtaaagtagc
      901 gcccgatgga accatcacca ccatagcggg cgccgggacg gatgatctca gcggcaatgg
      961 cggcctggcg ctccaggcgg agctgaactc gccgggcgga ctggctgtcg atgccgccgg
     1021 aaatttgtat atctccgaca cctacaacag ccaggtgcga aaagtgactc ctagcggcgt
     1081 cattaccgcc tttgccggga aaggaggcga gccggcgtat ttcggcgacg gagggcccgc
     1141 aacgctcgcc ggcttggctt tccccgccgg cctggcgttt gacgccgccg gcaatctcta
     1201 catcgccgat cagcagaata actgcatccg cagggtcgct cccgatggga tcatcacaac
     1261 cgtcgtgggc gatgggacgg gtgattttgg atttcggggc gacggcggcc cggcgaagca
     1321 ggcgcttctg aattatcctt cggatgttgc gttcgacgcc gaaggcgact tgtatattgc
     1381 cgatgaggac aacaaccgca ttcgaaaggt aagcccttct ggtattatta ctaccgtggc
     1441 gggcgacggg caggccattt tcaacggaga cggtgggccg gccgctaacg ccggcctgaa
     1501 tcgtcccagc ggtttggcat tcgacaaggg cggcaacctt tacgtcgttg atcaatacaa
     1561 ctggcggatc cgcaaaatcg ttttcaatcc gcccgtgctg tccgtttcgg ccgcgaaact
     1621 tgggttcgcc gccgaggcag gcggcccgaa tccggcgccg caaaccttta cagtcaccaa
     1681 tgcgggctcg ggaacgttaa acttcagcat tacgggcaat caatcctggc ttaccataac
     1741 gccggcgtca ggcgctgttt cctccggcgc atccgtcacg ataacggtca acgccagaag
     1801 tgcgaacctc aacgcgggcg attacgaagg cgcgctggtg atctccgctc cggggactct
     1861 cgagggcagc aggaccgtcc aggtcagcct ggcggtccgg gcggtgacgg cgccggggcc
     1921 cgtgttcacc gcggccggcg tggtcaacgc ggccagtttc gccagtgggc cgatcgcgcc
     1981 tggtgagatc atttcgatat ttgggtcgaa tctcgggcct gcggtgagca cgggcgcggt
     2041 gatcgacgcg tctacggcac gactggccac gattgtcgcg ggcgtcgccg tcctcttcaa
     2101 taaccttccg ggcgctcttt tctacgtgca ggagaagcag atcaatgtgc aggttcccta
     2161 tgaattagcg agccgcactg gcgccgtgcc gggcgtggtg acaatcgtgg tgagctatca
     2221 gggggcgtcg agcgctcccg tatccgttcc cgcggcggcg gctgctcccg cagtgtttac
     2281 cgtcagccgg ggtctggggc aggctgccgc cctgaaccag gacaattcgc cgaattccgc
     2341 cgccaatccc gccggccgag gcagcgtggt acagatcttc atgaccgggc aggggccgac
     2401 gaatccgccc gcgctgaccg gccagttgac tcagccgcct tatgccgcgc cactgcaaac
     2461 ccctacggtt acaatcgggg gtattaccgc taaaacgatt tttgtcgggc tagctccgaa
     2521 tctggcaggg ctgctgcaaa tcgacgccat cgtgccggtg gatgtcgtac ccgggccgaa
     2581 tgtgcctctg gaaatctcga taggcgcggc ctcgacacaa acaggagtga cgctggcggt
     2641 gcgatgagga gcagcccggg ctggcgggga tgaacccaga cccggcgaaa atccccggac
     2701 tcggcttgca acggagcaac ggcaaaaccg ccgcgaggag ccggtacgtg gtcgggtggg
     2761 aactgctctg aaagcagatc gaaatcgatc ttggtcttat gacgcgtcta caaaatctct
     2821 ttgatttgaa aggccgtgtg gccctggtca ccggcggatc tcgtgggctc ggacgcgaga
     2881 tggccgaggg cctggccgag gcgggcgctt cggttatgct ggtggcgcgc cgcgagcagt
     2941 ggctcaagcc tgctgtggcg gagatgaaag cggcgggctt cacatgcgag ggcatgctgt
     3001 gcgacgtctc ggtccccgac caggtggatg cagccgttac acgcacctgt gaggttctcg
     3061 gcggcataga tattctggtg aacaatgcgg gcgtcacctg gggcgcgccg attgaacaga
     3121 tgccgttaga caaatggcgc atggtccttg ataccaacct gactggcgca tttctggtgg
     3181 cgcaacgcgc ggcgcgagtc atgttggccc gcaagaacgg cgtaattatc aatgtggcct
     3241 cggtggcggc gctgaaagga atagcggaac tgggctacag cacgatcggc tatatcgcca
     3301 gcaaggctgg gctgatcgcc atgacgcgag agatggccgc ccgctggggg cggagcggaa
     3361 tccgggtaaa cgcgattgcg ccggggtttt ttccttctcg catgacagag aaggttttgg
     3421 aacgcattca agcggaatac gagaagcgca cgcccctggg ccgtgtcggc cgcgaggggg
     3481 aattgaaggc tgtcgccgtt tttctggctt ctccggcttc gagctacatc accgggcaga
     3541 cgattgtggt ggatggggga gcctccatcg tgtgagcgcc cgggagctca aagacaaaca
     3601 gaggaaggct gttgaaagct ggggcggcga cgtctgcgtc gtggccggcc cgggctccgg
     3661 caagacgttc gttctggtgg agcgcttccg atggttggtc ctcgagaaga atatctcgcc
     3721 ccaccgcatc ctggcgatca ctttcacgga aaaagccgcc gccagcatgc gccggaaact
     3781 gatcgagtcg tttccggccg gcagccgcca ccgggcggaa gtcgagcgtt cgtgtatctc
     3841 gacgattcac ggattttgcg cgcgcctgct gcgggaaaac gctatcgcag cggaagtaga
     3901 tccggagttt cgcgtgcttg atgagtggga agctgatttc gagttgcgcc gggccatcga
     3961 cggggctctc gagcaggcat attcagccga gccggagcgc gcgcggcgct ttctggccgc
     4021 gttcgcgtcg gaccaggttc acgagagcct gttctgcctc taccaggcgc tccgcgcggc
     4081 cggcgcaagc atcagtcaag caagccgcat tccgccctct tgcagtcttc agcaaagctg
     4141 ggcggctctc gtccaggcat tcgatcagac ggcggcctgc tctacagccg ggtggaacac
     4201 caaccagcgg gatgcgctcg gcgatgcgct cgccgtccgg aaacggcttg ccgagctcgc
     4261 aggcctcccg gcgtcgatcg cgcacttcca ggccctccgc caaatcgatt ttgttgtaac
     4321 agctttcaag cggggaagcc ggcaacggga actgctcaca cagattcgcg acgaactggc
     4381 gcctcgttgc cggcgcgcct tgcttctgga ggcgaacgcg gagaaccggc aatggttctt
     4441 ggagacactc tcgaaagcgg acagcctcta tcgcgccggc aaacagcaaa gggcgatgct
     4501 ggactatgcc gacttagagg aatatgccat ccggctcttg gagcgcacgg gcgggcggat
     4561 tgcggccacc ttcgaccaca ttctcatcga tgaatttcag gatacgaacg cgctgcaatc
     4621 gcggctggtc agccgtcttc ggcaaggcgc caggctgttt gtcgtggggg atatcaatca
     4681 atcgatttac gggtttcggc atgccgatcc tgaggtgttc cgcgcctatc gggagcagac
     4741 ccggagcggc ggcggtgaag tgcacgaact ggccgagaat tttcgcagcc ggcaagaggt
     4801 gctggacgcc atcaaggcga tcgcccggga cgctccaggc atcgaggtcc aggagctcac
     4861 cgctggcact cgctttttga cggcgagcca gccttgtctc gaggtccttg tagtgcacgc
     4921 tgaggacggc gacagagcct tccatttgga agcgcagcac atagcggccc gtattcggga
     4981 gctttgcggg catttaacgc tcaaagccgg ggaagagaat cccggcgggg aaccccagac
     5041 cagagtcgcc ggatacggcg attttgctgt cctgtgccgc acttcgatgc agctaagaat
     5101 catcgagcaa gtgttgcgcg accaaggcct cccctgtcag atcaccgagg gccgcgggtt
     5161 ttatgacacg cgcgaggtca acgatctgat ccactttctc cgggcgctgt ggaatccatt
     5221 ggacgagatc agcctggcgg ctgtactgcg ctcgccgctg gcgggcatca gcgacgagag
     5281 cctgtttcgg gcgaagctgg cggctggatc gctgcttggc ggattgcggc acgggttgga
     5341 cgacaatgcc gctgcgccgc attgcaagct gccgccggat gaagcggcca aagtaaagag
     5401 ctttctaacg cttttcgacc gctggcgcgc ccggcgcgac tacatgacga tcgaccgcct
     5461 gctggcgggc gtgctggcgg acacagggta tttcgagcaa ctgctgcggc ggccaggcgg
     5521 gccacagaaa gccgcgaatg tgagcaaact gatggcgctg gcccggcgtt ttcacgccag
     5581 cgggcaggcc ggactcgacg agttcattga gcgagcgcaa cagttgcggc gatttcag
//
LOCUS       DASREK010000061         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2012860, whole genome shotgun sequence.
ACCESSION   DASREK010000061 DASREK010000000
VERSION     DASREK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012860"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1889
                     /locus_tag="VFA54_03555"
     CDS             <1..1889
                     /locus_tag="VFA54_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49910.1"
                     /translation="LSPDIKINLPFTVTLARVPSLSFTSGSGIAGFGPYRDYNRNHAV
                     YDNFTWIKGRHMMKYGLTINHYQKTENAGGNNVGTFSFTNNGAPPGTPAFAQAWANFL
                     LGNVATFTQASLDLTPDIRTWQWEAYAQDDFRIRRNLTLSLGLRYSAFRQPIDAKNML
                     TAFDPAVFNPANAPKIDPATGNLVSLSSGNPLNGVIINKSDAQYGNSPYGNKVANENN
                     GNFAPRIGIVWDPFGKGQTSIRTGYGIYYDSILFGIYEQNIFNNPPYVSSISIPNTRL
                     ENPTAGTPSVSLNPKVIRSTPVPYRTPYVQQWSFDIQQQLGKNFILDVGYFGSKGTHL
                     LGIVDINEVYPGVGFAAGLHTGPGTAFTSADTPRLNAVRPYLGYNVINSIETWFNSNY
                     NSLQVALEKRFGAAGVFNLAYTWSHNLTDNQSDRSNAPQNTYDWHNGEYGPAALDKRH
                     VLTLGYVYELPIFKTATGVKKYALAGWQISGITSFAAGSPLTAATSNVDPAGLGILGS
                     SAAGPRPDMICDPNANAPHTVAQWFNTACFVNVPAGEIRPGNSGRGVILGPGAQTWNF
                     SLFKNFPFTERWRAQLRGEFFNVFNHPNPLGLGSTNITSSLYGRITSFHDPRIVQIGA
                     KLYF"
     gene            2137..>2768
                     /locus_tag="VFA54_03560"
     CDS             2137..>2768
                     /locus_tag="VFA54_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49911.1"
                     /translation="MIGGIRNYGKVVDWGPIDWEEKMTSISRTRYRLCLCLGLTLCAA
                     ATAQTPKPKRINRAIELLEQGQPIYYTGSHEGTAGSFEQGKKDAQTYADYISYDMEHA
                     PFDVKGLAEYMRGLAAGGPTKSGHRTPAVIVNVPVDGLDEAAVHANAWMFQQVLATGV
                     HGILLCHADTPGAVRAFVEAVRFPIHKQAVGQGLSEGRRGAHGTPTAARI"
BASE COUNT          595 a          863 c          772 g          538 t
ORIGIN      
        1 cgctgtcgcc cgacatcaag atcaacttgc cttttacggt gacgctagcg cgcgttccgt
       61 cactgagctt caccagcggc tccggtatcg ccgggttcgg gccatatcga gactacaacc
      121 gcaaccatgc ggtatacgac aacttcacct ggatcaaagg ccgccacatg atgaagtacg
      181 ggctcacgat taaccactac caaaagactg aaaacgccgg tggcaacaac gttggaacct
      241 tttctttcac taacaacggc gctcccccgg gcacgcctgc cttcgcacaa gcctgggcca
      301 actttctgct gggcaacgta gccacattta cccaggcgtc cctcgatctg acaccggaca
      361 tccgcacctg gcagtgggag gcctatgcgc aggacgactt ccggatccgg cggaacctca
      421 ccctcagcct tggcctccgc tactcggcat ttcgccagcc gatcgacgcg aagaacatgc
      481 taacggcctt cgatccagcg gtgtttaacc cggccaatgc ccccaaaatc gatccggcga
      541 ctggcaacct tgtatccctc agctctggaa acccgctgaa cggagttatc atcaacaaat
      601 ccgatgcgca atacggcaat tcgccctacg ggaacaaggt ggccaacgag aacaacggca
      661 acttcgcgcc acgcatcggt attgtctggg atccgttcgg caaaggccag acttcaattc
      721 gcaccggcta tggcatctat tatgactcga ttctgtttgg tatctacgaa cagaatattt
      781 tcaataatcc gccgtacgtg agctcgatca gcatccccaa cacgcggctg gagaacccta
      841 cagccggcac gcccagcgtt tctctgaacc caaaggtgat ccggagtacg ccggtgcctt
      901 accggacgcc gtatgtgcag caatggagct ttgacatcca acagcaacta ggcaagaact
      961 tcatactgga cgtcggatac ttcggctcca agggaactca cctgctgggc attgtggaca
     1021 tcaacgaggt atacccgggt gtcggcttcg cggccggcct ccacacgggc ccgggtactg
     1081 cattcacaag cgctgatacg ccccggctaa atgccgtccg gccgtacctc ggatacaacg
     1141 taatcaatag catcgagacc tggttcaatt ccaattacaa ttccctccag gtcgcgttag
     1201 agaagaggtt cggcgcagca ggggttttca atcttgccta cacttggtct cacaatctga
     1261 ctgacaacca gtcggatcgc tcgaacgccc cgcagaatac gtacgactgg cacaatgggg
     1321 agtatggccc tgcggcgctc gacaagcgcc atgtgctcac gcttggctac gtttacgaat
     1381 tgcccatatt caagacggcg acgggcgtga agaaatacgc gctggccggc tggcaaattt
     1441 ccggcatcac gagctttgcg gccggctctc cgctaaccgc cgcgacttcc aatgttgacc
     1501 cggcgggact cggtattctg ggctccagcg cagccggtcc acgcccggac atgatctgcg
     1561 accctaacgc caatgctccg cacactgtag cccaatggtt caacactgct tgctttgtca
     1621 atgtgcctgc gggcgagatc aggccgggga attcgggccg cggcgtgatt cttggtcccg
     1681 gcgcccagac ctggaatttc tcactcttca aaaattttcc gttcaccgag cgctggcggg
     1741 cgcaactacg gggcgaattc tttaatgttt tcaatcaccc gaatccatta ggccttggca
     1801 gtaccaacat caccagttcg ttatacggac ggattacctc gtttcacgat ccgcgcatcg
     1861 tccagatcgg agcgaaactg tatttttgaa gcacttgcat ccttatgacc cggtcagcgc
     1921 gccgccgagc cgcaggcggc tggcggccgc caggtaccgg ggtgatgcaa ccgggatgct
     1981 ggtggagatc cggactggcc acgtcctact tcgaacccgc ctgtttgccg gtttgttccg
     2041 gccgcgatgt ttgtgcggat tctggcgccg gagcttctcc ccgcgagaag tgaagaaacc
     2101 ccggaatgga ctggtccgca ggcgggcgga aggtgtatga tcggtggcat tcggaattat
     2161 ggaaaagtcg tggattgggg ccccatcgac tgggaggaga aaatgacgag catcagcagg
     2221 acgcgatatc gcctatgcct gtgtctgggc ctgacactct gcgccgcagc cacggcgcag
     2281 acgcccaagc ctaaacggat caacagagcc attgagctgc tcgaacaagg ccagcccatt
     2341 tattacaccg gctcccatga gggcacggcc ggaagctttg agcaaggcaa aaaggacgcc
     2401 cagacgtacg ccgactatat cagctacgac atggagcatg cgccctttga cgtgaagggt
     2461 ctggccgagt acatgcgcgg gctggcagcc ggcggcccga ccaagagcgg gcaccgcacg
     2521 cccgcggtca tcgttaacgt gcccgttgac ggccttgacg aggcggcggt ccacgcaaat
     2581 gcgtggatgt ttcagcaggt gctcgccacg ggcgtccatg gcattctgct ctgccacgcc
     2641 gatacccccg gcgcagtccg cgccttcgtc gaagccgtgc gcttcccgat ccacaagcag
     2701 gcggtaggtc agggactgag cgagggccgg cgcggcgctc acgggacgcc cacggcggcg
     2761 agaatctg
//
LOCUS       DASREK010000062         5672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_34224, whole genome shotgun sequence.
ACCESSION   DASREK010000062 DASREK010000000
VERSION     DASREK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34224"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFA54_03565"
     CDS             <1..288
                     /locus_tag="VFA54_03565"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HZS49912.1"
                     /translation="DEQVGAAVRSLHDQCREALSRYVKLEPVIDSVEGTFTNTASVDA
                     KAVKFVGNLPAGTPSGGILRHKGWRASKVDLPALTPKQDASILAPAEIEVE"
     gene            295..2124
                     /locus_tag="VFA54_03570"
     CDS             295..2124
                     /locus_tag="VFA54_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZS49913.1"
                     /translation="MEPKYVIGIDLGTTNSALAFTEIQPGAGPLDIPKVDLFAIPQVV
                     NPGEVREEGLLPSFLYLPGGSDFPAGSTALPWDATPDFVVGRLAQKRGVENAGRLVSS
                     AKSWLSHGGVDRTAPILPWRAPEGVPKLSPVEAGRRYLEHMQSAWDYKMPAAPFAEQQ
                     VLVTVPASFDAVARDLTLKAAELAGYRNLTLLEEPQAAFYAWIERHPHWREQVKVGDL
                     ILVIDIGGGTTDFTLIAVTERSGELALERVAVGEHILLGGDNIDLALARAVADGLSAR
                     GTRIDAFQLQTLWNNCRAAKEKLLDPNNTAEEEPVTILGKGTGLVGGTIKASLRREQV
                     ERILDEGFFPAVASTDMPARQRRVGLQEIGLPYAADAAVTRHLARFLRQQPANVEHGS
                     IRRGASGLACPTHVLFNGGVLNAGLIRRRLIEVLDSWLAEESMAPVTLLSGEDLMHAV
                     SRGAAYYGLARRGKGIRIRGGVPRTYYVGIESAMPSVPGLAAPLKALTVVPFGMEEGT
                     DLQIPDREFGLVVGEPAEFRFFGSAVRKNDQPGDLIEDFGDELQELAPMEVNFEAAQG
                     SADIVPVSFEAVVTETGMLELWCVARDGRRWKLEFNVRERVEA"
     gene            2121..4799
                     /locus_tag="VFA54_03575"
     CDS             2121..4799
                     /locus_tag="VFA54_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZS49914.1"
                     /translation="MKVGIDLGTTNSALAYIDEREAEDRDFPPIHIFAVPQLVAPGRT
                     ESRRTLPSFLFLEDGEPVGVYAREQGAVVPTRLVHSAKSWLSNPDVDRTARILPWDSP
                     ETGRVLSPVEVSTRFLTKFRESWDAVRAVPLAEQDIVLTVPASFDEEARELTVMAARD
                     AGLERLTLLEEPAAAFYSWIANNLARSHKLLFNGQIVLVCDVGGGTSDFSLIRISRQG
                     DLVDFTRTAVGKHLLLGGDNLDLTLAWLVETKLGVPLSIRQRSGLRRQCSAAKERLLN
                     DPNLKSVEITVLGAGSALIGKSLKTEILREEALELALEGFLPLTPRGEAPKEEKRSLF
                     RELGLPYVSDPAITRHLNAFLESAGAAPDAVLFNGGFFIPEILRQRVADVIGHWYGKR
                     PEILENRDLDLAVAAGAAYYSYVRFTGSGVLVRGGLPRTYYIGISDGTGDRISTVCLV
                     PRGAEEGATLEIDRGDSLQLVANRPVSFRLYSSLTRTDDRPGDTVEFAAGDPDLHAHA
                     PLHAVIRFGKKTGERLIPVKLGARLTEIGTLETWCESKISDNRWRLQFELRKSATPQP
                     AERKAAAVVSEEALGQALEVIPAVFSSGGKAPITPEELPTRLEQLLGLGRNSWPGETI
                     RRMADAFLASADGRKKSPAFEVRWLNLCGFCLRPGFGYPGDDFRIEQTRRIYAGGLQY
                     SNQVQCEIEWWIFWGRVAGGLNRNQQIDLYQRLSGALLPRGGKKHRLNTSLLREMWRT
                     AASLELLPVGTKTELGEALVRKIKSGEYRETDLWCISRLGARELFYGPMNLVVPPAVA
                     ARWVEALLAVDTAGDALAALARRTGDPARDVSTSTFAAVRRRLEGLPNAARYLAVIEG
                     DEERDERSLGRIFGEELPSGLVLRGDEAAEDETGTL"
     gene            complement(5250..>5672)
                     /locus_tag="VFA54_03580"
     CDS             complement(5250..>5672)
                     /locus_tag="VFA54_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HZS49915.1"
                     /translation="DKAYNDSAIWVDFTKDDKPRHFRLGVFGALQSWNPQNLPPDKNP
                     AFLQRLVVVKQPPFDRGKWTHVGIVFADLGKGGNAKLFVNGKLQGATPAIPEPFQWDV
                     ARAAIRLGVNYVGLLDDVAIFRRPLTDQEIGTIASQRF"
BASE COUNT         1041 a         1745 c         1800 g         1086 t
ORIGIN      
        1 gacgagcagg tgggagccgc ggtccgcagc ttgcacgatc agtgccgcga ggccctttca
       61 cgctacgtga agctcgaacc ggtgatcgac tcggtagaag gtacatttac caacaccgcg
      121 tcagtcgacg ccaaggcggt caagttcgtc ggcaatctac ccgccggcac gccttcggga
      181 ggcatattgc gtcacaaagg ctggcgggct tccaaggttg atctgccggc gctcaccccc
      241 aagcaggacg cgtcgattct tgcgccggcg gaaattgagg tcgagtaaca gagtttggaa
      301 ccaaaatacg tcatcggcat tgatctcggc accactaatt ccgctctggc ttttacggag
      361 attcaaccgg gcgccggtcc tttggacata ccgaaagtcg atctgttcgc aattcctcaa
      421 gttgtgaacc cgggcgaggt ccgcgaggag ggcttgttgc cgtcgttcct gtaccttccg
      481 ggcggcagcg actttcccgc gggctcgacg gcgcttccct gggacgcaac cccggacttt
      541 gtagtcggac ggcttgcgca gaaacgcggc gtggagaacg ccggccggct ggtgtcctcg
      601 gccaaatcgt ggctgtccca tggcggcgtg gaccgcacgg cgccgattct cccctggaga
      661 gctcccgagg gggtacccaa actgtcgccg gtcgaagctg gccgccgcta cctggagcac
      721 atgcaaagcg cctgggacta caagatgccg gctgctccgt ttgccgagca gcaggtgctt
      781 gttacggttc cggcgtcgtt cgatgctgtg gctcgcgatc tcacgctgaa agcggcggag
      841 ctcgccggct accgcaatct cacgctgctc gaggagccgc aggcagcgtt ctatgcctgg
      901 atcgagcggc acccgcattg gcgcgagcag gtcaaagtgg gcgatctcat tctggtgatc
      961 gacatcggag gcggcaccac cgacttcacg ctaatcgccg tgaccgagcg ttcgggcgag
     1021 ctggcgctgg agcgcgtggc ggtcggcgag cacattcttc tcggcggcga caatatcgac
     1081 ctggcgctcg cgcgcgcagt agccgacggc ctcagtgcgc gcggcacccg gatcgatgcc
     1141 ttccagcttc agacgttatg gaacaactgc cgcgcagcta aggaaaagct gcttgatcca
     1201 aataacaccg cagaagaaga accggtaacc atcctgggca agggcacggg cttggtcggc
     1261 ggcacgatca aggcttcgct tcgccgcgag caggtcgagc gcattctcga tgagggcttt
     1321 ttcccggccg tcgcgagcac cgatatgccg gcccggcagc ggcgcgtagg cctgcaggaa
     1381 atcggccttc cgtatgccgc cgatgcggca gtcacacgtc acctggcccg ctttctgcgg
     1441 cagcagcccg ccaacgtaga gcacggctcg atccgccgcg gcgcaagtgg tctcgcctgc
     1501 ccgacgcatg tgctgttcaa tggcggtgtg ctcaatgccg ggctgatccg ccgccgcctg
     1561 atcgaggttc tggattcgtg gctcgccgag gagagcatgg ctccggtgac gctgctcagc
     1621 ggtgaggacc tgatgcacgc ggtttcgcgc ggcgccgcat attacggcct ggcgcgccgc
     1681 ggcaagggta ttcgcattcg cggcggagtg ccgcgcacat actatgtcgg tatcgagagc
     1741 gccatgccgt cagtgccggg tcttgccgcg cctctcaagg ctttgaccgt cgttccattc
     1801 ggcatggaag aaggcacgga cctccagatt ccggaccgcg agttcggcct tgttgtcggt
     1861 gagcccgccg agttccgttt cttcggctcg gccgtgcgca agaacgatca gccgggcgac
     1921 ctcatcgaag acttcggcga tgagcttcag gaactggcgc ccatggaagt gaacttcgaa
     1981 gctgcgcagg gctccgccga tatcgtgccg gtctccttcg aagccgtggt cacggagacc
     2041 ggcatgctag agctctggtg cgtagctcgc gacggacgcc gctggaagct ggaattcaat
     2101 gttcgcgagc gtgtcgaggc atgaaggttg gaatcgatct cggcactacc aattcggccc
     2161 tggcttatat cgacgagcgg gaagccgagg accgcgattt tccgcccatc cacatctttg
     2221 cagttccgca gcttgtagcc ccgggacgta ccgagagccg gcgcacgctt ccttcgtttc
     2281 tcttcctgga agatggagag ccggtcggcg tatatgcccg cgagcagggc gccgtagtgc
     2341 cgactcggct ggtacactcc gccaaatcgt ggctgtcgaa tccggatgtc gaccgtaccg
     2401 cgcgcattct gccgtgggat tcgcccgaaa ccgggcgcgt gctgtcgccc gttgaggttt
     2461 ccactcgttt cctcacaaaa ttccgcgaga gctgggacgc ggttcgcgcc gtaccgcttg
     2521 ccgaacagga tatcgtcctc accgtgccgg catctttcga tgaggaagcc cgcgaactca
     2581 ccgtaatggc cgcgcgcgac gccggtcttg agcggctcac actgctcgag gaaccggccg
     2641 cggcgttcta ctcctggatc gccaataatc tggcccgttc ccacaagctg ctctttaacg
     2701 gacaaatcgt gctggtatgc gatgtgggcg gcggcaccag cgatttcagc ctgattcgta
     2761 tctcgcggca gggcgaccta gtggacttta cgcggactgc cgtgggcaag cacctgctgc
     2821 tcggcggcga caatctcgac ctgaccctcg catggctggt tgagacgaaa ctcggcgttc
     2881 ctctttcgat ccgccagcgc agcggcctgc gacgacagtg ctcagcggcc aaggagcggc
     2941 tgcttaatga tccgaacttg aaaagcgtgg aaatcacggt cctgggcgcc ggctcggcgc
     3001 tgatcggcaa atcgctcaag accgaaatcc tgcgtgaaga ggcgctcgaa ctcgcgctgg
     3061 agggctttct cccgctcacg ccgcgtggcg aagcaccgaa ggaggagaag cggtcgctgt
     3121 tccgcgagct gggcctgccg tatgtctccg acccggcgat tacccgccac ttgaatgcat
     3181 ttctcgagag cgccggggcg gctcccgatg ccgtgctgtt caatgggggc ttctttattc
     3241 ccgagattct gcggcagcgc gtggcggacg tgatcgggca ctggtatggc aagcggcccg
     3301 aaatcctgga gaaccgcgat ctcgatctgg cggtggctgc cggcgcggcc tattactcat
     3361 acgtccggtt caccggcagc ggcgtgctgg tgcgcggcgg cctgccgcga acctactata
     3421 tcggtattag cgatgggacc ggcgatcgga tttccacagt gtgcctggtt cctcgcggcg
     3481 ccgaggaggg agcgacgctg gagattgacc gcggcgatag cctgcagctc gtggcaaacc
     3541 ggccagtttc cttccgcctt tacagctcgc tgacgcgcac cgacgaccgg ccgggcgaca
     3601 cggtcgagtt tgcagcgggc gatccggacc ttcacgctca tgcgccgctt catgccgtca
     3661 ttcgcttcgg gaagaaaacc ggcgagcgcc tgattcccgt gaagctcggc gcacggttga
     3721 ccgagatcgg cacgctggag acctggtgcg aatccaagat cagcgataat cggtggcgcc
     3781 tccagttcga gctgcgcaag agcgccacgc cgcagccggc cgaaaggaaa gcggccgcgg
     3841 tggttagcga agaagctttg gggcaggcgc tggaggtcat tcccgccgtg ttttcttccg
     3901 gcggcaaagc gcccatcacg cccgaagaac tgcccacgcg tctcgagcag ttgcttggct
     3961 tgggccggaa ctcctggccc ggcgagacta ttcgccggat ggccgatgcg tttctggctt
     4021 ctgccgacgg ccgaaaaaag agcccggcgt ttgaagtgcg atggctaaat ctgtgcggct
     4081 tctgcctgcg ccctggattc ggctatcccg gcgacgactt ccggatcgag cagacgcgcc
     4141 ggatttacgc gggcgggctc cagtactcga accaagtgca gtgcgaaatc gagtggtgga
     4201 ttttttgggg acgtgtggcc ggcggcctca accgcaacca gcagattgac ctgtaccaga
     4261 ggctttccgg cgcgcttctg ccgcgcggcg gaaagaagca tcggctgaat accagcctgc
     4321 tgcgcgagat gtggcgcacc gccgccagcc tcgagctgct gcccgtcggg acgaaaacag
     4381 aacttggcga ggccttggtg cgaaaaatca aaagcggaga gtaccgcgag acggacttat
     4441 ggtgcatctc gcggcttggc gcgcgggagc tgttttacgg cccgatgaat ctcgtagtgc
     4501 cgccggcggt tgctgcccgc tgggtggaag cgctcctggc ggttgatacg gccggggacg
     4561 cgttagctgc gctcgcgcgg cggaccggcg accctgcgcg cgacgtttca acctccacct
     4621 tcgccgcagt ccggcgcagg ttggaaggat tgcccaacgc tgctcgatat cttgccgtca
     4681 tcgaagggga cgaagaaaga gacgaacgca gcttgggaag aatctttggc gaagaactcc
     4741 cttccgggct ggtcttgcgc ggcgacgaag cggcggaaga cgaaaccggc acactatgag
     4801 gagcgtagtg ccatacgctt aaagacggcg caccggattg ctccggtact tccgacgcat
     4861 tctgccgggc cggttgcggc atctgcattc gttttagcca ttgcgtcacg ccggccacca
     4921 ccaatcctac aagtccgatt gcagccgggt ccgataaccg gacgacaggg gcacccgcta
     4981 gcacgatccc ggcggcggcc aggaccacgc aaaccaacag caggacagag ccgaaatctc
     5041 ggcccctggt tcgatgcatg aggctgattg ctcccttctg gcttggcgta atcaacgtta
     5101 attgatgcaa ttgccgtacc gggagataag tcgttttgtt tattcctctc ccacgagagg
     5161 aatacccaaa aaatcgagct tggtgccgcg ttttacgact tgttcccagc gtgtaaccgg
     5221 cttgtcacct ggcgcctgat gcttctgagt cagaaacgct gagatgcaat ggttccaatt
     5281 tcctggtccg tgagcggccg gcggaaaatg gctacgtcat ccaaaagccc cacgtaattc
     5341 actcccagac gaatcgcggc tcgcgcgaca tcccactgaa acggctccgg gatcgcggga
     5401 gtggcgccct gcagtttgcc gttgacaaaa agtttggcat tgcccccctt tccgaggtcc
     5461 gcgaagacaa tgccgacgtg cgtccacttg ccccggtcga atggaggctg tttcaccacg
     5521 accaacctct gcagaaaagc aggattcttg tcgggcggca agttctgtgg gttccaggac
     5581 tgaagcgcac cgaaaacacc caagcgaaaa tggcgcggct tgtcgtcctt ggtgaaatcg
     5641 acccagatgg ctgaatcgtt ataagccttg tc
//
LOCUS       DASREK010000063         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1023271, whole genome shotgun sequence.
ACCESSION   DASREK010000063 DASREK010000000
VERSION     DASREK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023271"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFA54_03585"
     CDS             <1..370
                     /locus_tag="VFA54_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49916.1"
                     /translation="QIEQYLRTGAYEQPSDADRYQMIRSYYRMLLLREGRNDPETTGK
                     MKQFASYFTHGVRNGSHLRSEIYHARTPDAIYDCVERFFASLLGHGSGEEPLPQKAAS
                     SRPHDAAAPHFSEDAPAGVR"
     gene            367..843
                     /gene="rnhA"
                     /locus_tag="VFA54_03590"
     CDS             367..843
                     /gene="rnhA"
                     /locus_tag="VFA54_03590"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZS49917.1"
                     /translation="MKHVTLITDGACLGNPGPGGWACILKCDGHRKEIYGSEPHTTNN
                     RMELMAVIEGLRALKKPCRVTIITDSQYVKNGITNWIHGWKRKGWITSARTPVLNKEL
                     WMALEGEIKRHQVSWEWVKGHAEHADNMRADELAARAAREQSRSLAGTPGIGEVIN"
     gene            complement(1113..1715)
                     /locus_tag="VFA54_03595"
     CDS             complement(1113..1715)
                     /locus_tag="VFA54_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS49918.1"
                     /translation="MSNNRLNRREMLMLAGAAAIRPAHADAPLLRPRRIDHVSLAVTD
                     VDQAMIFYRRLFGNEVLKENKTPRRYIRLGPGYASIAPAASGQAVRIDHYCAGIDDFQ
                     GNALKSSLASAGIPVRGSGGGLFVTDPDGTSLQLMADGSWTHLGSNAGPETGLSGEPL
                     FRARGMHHLAIRVSNMERSTEFYRKLFGPEVRRQGDPPQP"
     gene            2054..2626
                     /locus_tag="VFA54_03600"
     CDS             2054..2626
                     /locus_tag="VFA54_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZS49919.1"
                     /translation="MRITPTLTICTLLLTAGLGSAMAQDKKAPPGPGLTLTTTAFADG
                     GEIPSKFTQNDPHPVSPKLEWTHVPANTAAFVLIMHDPDVAVQRRTEDVLHWMVFNIP
                     GTARELPEGVPPNPQLPDGSVQAKNIGGVVGYRGPGAPAAGPHHHYTFELFALDTKLD
                     LGPDATRADVLRAMDGHILGKGVLVGRFHR"
     gene            complement(2882..>3291)
                     /locus_tag="VFA54_03605"
     CDS             complement(2882..>3291)
                     /locus_tag="VFA54_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49920.1"
                     /translation="IWGVSQQEYLQKADVWPLNPNGELMLGLKLEDKYALANAEESTK
                     VPGIAFAEWGPGDMALSLGVRGGGGRGPMPPEMVAARARVFAACKANHLFFLNSMTPA
                     DVIDMIKEGVMIGPASPQAAEIGRKYTKREMPW"
BASE COUNT          675 a         1037 c          984 g          595 t
ORIGIN      
        1 gcaaatcgag cagtatctaa gaaccggcgc ctacgagcag ccgagcgacg ccgaccgcta
       61 ccagatgatc cggtcttatt accggatgct gcttctgcgc gagggccgca acgaccccga
      121 gactacgggc aaaatgaagc agtttgcgag ctatttcact cacggcgtgc gcaacggctc
      181 gcatctgcgg tccgagatct atcacgcccg aacgcctgac gcgatctatg attgcgtcga
      241 acgctttttt gcttcgctct tgggacatgg ctcgggcgag gagccgctgc cgcaaaaggc
      301 agcttcatcc cgcccccacg acgccgccgc gccgcatttc agcgaagacg ctcctgccgg
      361 cgttcgatga agcacgtaac gctgatcacg gacggcgcct gtctcggcaa tcccgggccc
      421 ggaggctggg cctgcatcct gaaatgcgat gggcacagaa aagagatcta cggctcggaa
      481 ccccatacga ccaacaaccg catggagctc atggccgtaa tcgaaggcct ccgtgcgctc
      541 aagaagccct gccgcgtcac catcatcacc gattcccagt atgtaaaaaa cgggatcaca
      601 aactggattc acggatggaa acggaagggc tggatcacct ccgcccgaac gccggtgctc
      661 aacaaagaac tttggatggc gctggaaggg gaaattaaac gccaccaggt gagttgggag
      721 tgggtgaaag gccacgcgga gcacgccgac aatatgcgtg ccgacgagct tgccgccaga
      781 gctgcacggg agcagagccg ctcccttgct ggaacgcccg gcatcggtga ggttatcaat
      841 taaaccccca gcgcaggggc cagccctcgc ctctggcgct agcctttttt ggcaggcgca
      901 gccgtcatga cttgcagccg gattccctgc ggatcgtaaa attccgggag cgttccgttc
      961 ctcgacagtt ctgcttttgc gccgaaagcc tgcaactcct ttactgctca agccggatca
     1021 aagcgatcca ccaacacaca gaagtgatcg acgcccggat ctttgcctgc cgcggggtag
     1081 taaagtccca ccgtggtctc ccccgcctgg aatcaaggct gcggcgggtc gccttggcgc
     1141 cgcacttccg ggccgaacag ctttctgtaa aattcagtgg atctctccat attagaaacc
     1201 cggatcgcta agtgatgcat gccgcgcgcg cgaaacaaag gctctccgga gagccccgtt
     1261 tcgggacctg cattgctgcc aagatgagtc caggatccgt cggccatgag ttgcaggctg
     1321 gtcccgtccg gatccgtaac gaaaaggccg ccgcccgacc cacgcaccgg aattccggcg
     1381 ctcgccaagc tgctctttag ggcgttccct tgaaaatcgt cgatgccggc gcagtagtga
     1441 tcaatgcgaa cggcctgtcc ggatgcagcc ggagcaatcg aagcatatcc aggccccagg
     1501 cggatgtagc gccgcggcgt tttgttttct ttcagcacct cgttgccgaa taatctgcgg
     1561 taaaagatca ttgcctgatc gacatccgta acagcaaggg agacatggtc gatccgcctg
     1621 ggccgcagaa gcggcgcgtc cgcatgcgcg gggcggatgg ccgccgcgcc agcgagcatc
     1681 agcatttccc gccggttcag ccgattgttt gacatttgcg cctccctagg cggccaagcc
     1741 gcctttcgtt cggggccatt ctactcgttt ccggggcaaa gagccagggt cgcggtgtgc
     1801 cgtcaaaggc gatccgaaga ccgcagcaga gttgccgccc cggcgcggcg cccattggcg
     1861 ctccgtgcca atgccatgct ggaagacagc tccacgagtt ctttgcaaag tgaatggagg
     1921 cgtagcaccg taaggcagcg cagggagcgg ctgggggcaa aaacgagtcc tgcgggcaga
     1981 agttcggaaa tccggcaaag ccctctccaa gcgcccgccc tgctattctg atacaacgcg
     2041 aggaaggata gatatgagaa ttaccccaac actcaccatc tgcacgcttt tgctgacggc
     2101 cggtctcggc tccgcgatgg cgcaggacaa aaaagcgccg cccgggccag gtctgactct
     2161 gaccaccacg gcgttcgccg acggaggcga gatccccagc aaattcacgc agaacgatcc
     2221 gcacccggtt tcgcccaagc tggagtggac tcacgttccc gccaacacag ccgcgttcgt
     2281 tttgatcatg cacgatccgg atgtggctgt ccaacgcaga acagaagacg tcttgcattg
     2341 gatggtgttc aacattccgg gaacagctcg cgaacttccc gagggagtgc cgccgaaccc
     2401 gcagttgccg gacgggtcgg tgcaagcgaa gaacatcggg ggcgtagtgg gctacagggg
     2461 gccgggcgcg ccggccgcag ggcctcatca tcattacacg ttcgagctct ttgcactcga
     2521 tacaaagctc gacttgggac ccgatgccac gcgcgcggat gtgctgaggg cgatggacgg
     2581 ccacattctg ggaaaaggcg tgctggtggg ccgcttccat cgttagtacg aggtcgtcag
     2641 aggagcagcg gtccctgtcg cggctgtaag gattctccgg atgttacgtc catctccagc
     2701 cgaggcagcc gggcgcttcc gtgcagaggc cgctaaagcg caaagcgcac gcagcgcaca
     2761 cgacgctcgg ccaccccgta gctgcggcgg acctgtccca gcgtagccac ggtcccagtt
     2821 tgaaaaattc ccaagctgtg agaaccgagc cggcgccgca gggccggccg gctcgggagc
     2881 tttaccaggg catttcccgc tttgtgtatt tccggccgat ttccgccgcc tgcgggctgg
     2941 ccggcccgat catgacgcct tccttgatca tgtcgatgac gtcggcgggg gtcatcgagt
     3001 tcaaaaagaa aaggtgattg gctttgcagg ccgcaaagac ccgcgcccgc gccgccacca
     3061 tttcaggagg cattgggccg cgcccgccgc cgccccggac ccccagggac agcgccatat
     3121 cgccggggcc ccactccgca aaggcgatgc cgggaacctt ggtgctttct tcggcattgg
     3181 ccagcgcgta cttgtcctcc aatttaagac ccagcatcaa ctcaccattg gggttgagag
     3241 gccagacgtc ggctttttgc aggtattcct gctgggaaac tccccagatt c
//
LOCUS       DASREK010000064        11399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1851127, whole genome shotgun sequence.
ACCESSION   DASREK010000064 DASREK010000000
VERSION     DASREK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11399
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851127"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFA54_03610"
     CDS             complement(<1..502)
                     /locus_tag="VFA54_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZS49921.1"
                     /translation="MISSFRNVAIIAHVDHGKTTLVDAMLHQSGIFRANEQVVERVLD
                     SNDLERERGITILAKNTAIFYRGVKINIVDTPGHSDFGGEVERALKLVDGAMLLVDAS
                     EGPLPQTRYVLRKALEARLALIVVINKIDRPDARPSEVLDEIYDLFIDLDATEDQLNF
                     PVIYTNG"
     gene            952..1977
                     /locus_tag="VFA54_03615"
     CDS             952..1977
                     /locus_tag="VFA54_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZS49922.1"
                     /translation="MNLRSLFSVFSSDLAIDLGTANTLVFARGKGIVVDEPSIVAINK
                     NTGDIEAVGKEAKEMLGRTPGNIVAIRPMKDGVIADFKVTEKMLNYFIQKAHNRKMLV
                     HPRIVIGVPSEITQVEKRAVMDSAYRAQASEVHLVEQAMVAAIGAGLPITEPSGNMVV
                     DIGGGTTDVAVISMSGIVYSRSVRIAGNEMDEAIMQYLKRKYNLLIGERTAEQVKIEI
                     GSAYPLDKPLTMEIKGRNLIEGVPKTVTVDDSEIRESLSECVATIMNAIRVALERTPP
                     ELSADISDRGIVLTGGGALLRNLDKRIREETGLPVSIAENPLASVVLGTGKMLSDFKL
                     LRKISID"
     gene            2163..3443
                     /locus_tag="VFA54_03620"
     CDS             2163..3443
                     /locus_tag="VFA54_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZS49923.1"
                     /translation="METLLARYRNITVLALAIFAQLLLLGYQIRSDRDVRLIRVWGVT
                     AITPVARVVHGVHESVAALWSNYIWLRGARQQNERLRAEIGRLKLENQALRNSLEAAE
                     RLKILTAYQQNILSETTVASVIGAGANPNSRVIFLDKGAGSGVKTGMAVITPDGIVGR
                     VQSVFPGSSLVQLIDDTNSAVGVILETSRAHGILKGAGLNEGRIDYVPNEEKVDPGER
                     VYTSGEDRIYPKGLLAGIVLECRQGRDFKEITVRSAARLDRLEEVLVVTRGVHQDLPR
                     KVPKPGAPEVLLPPPPIEDRDGLSGLSAPRTAVAGGAGPAGSAPSLPADGSVAGTDAD
                     RVKRRYEELGAMQGHKFGEGAPGSKPPDFNLGWSPQGGMALRRPPAAKPPGPSGSADA
                     PGSGAGSLAPRVPGTNAPPQPPDKPGAAPGSVRP"
     gene            3445..3975
                     /gene="mreD"
                     /locus_tag="VFA54_03625"
     CDS             3445..3975
                     /gene="mreD"
                     /locus_tag="VFA54_03625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZS49924.1"
                     /translation="MQASVKQLVEADINRLRPWIAIVAVLGALLLQAYLPLLLPLFSS
                     YANMADLPLLVTIYLALLRRSPVAGLSIGMGVGLAQDSLSDGPIGMYGIIKTIIGYIC
                     SSLTQVIAVEPLGTRVILVFGCYLIHQAVFWVMERVLLGQAVAFLWQRALFLAVINAL
                     LALLLFRFLDKFRERI"
     gene            3979..5871
                     /gene="mrdA"
                     /locus_tag="VFA54_03630"
     CDS             3979..5871
                     /gene="mrdA"
                     /locus_tag="VFA54_03630"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZS49925.1"
                     /translation="MATLNPLDPRKAQFAKFAQGRIALIQYLCLFVFLYLISGFWELQ
                     VQNPDVYAEQAERNRVKWLPLLAPRGKILDRENRVLADNHSTFSVILSRSNFDEKHLP
                     VIADGLNVSLPDLQARLRRSRNEPEYEALVIKNGLTEAEVAFVEAHHQELPELELVRS
                     QRRLYPADGFASHLLGYVGEVSEEELNQPEFAMYNPGAVVGKAGIEREYNDVLTGIDG
                     QRQVIVDSRGRERGVIGIKEAKPGRSIRLTIDLDLQAAAELAMQGRTGAVVALDPRTG
                     EVLALVSTPNYDPNKFAGHISAADWKELVSNPDNPLLNRAIQAQLAPGSTFKPFVALA
                     GLESKTIDEDFSVHCGGGASFYGRYFKCWVKTGHGAVSLHKAIRESCDVYFYNVGNKT
                     GIDTIAQYAEAAGFGRRTGIDLPEEKDGVMPSSKWKLRNFRQKWYAGETISVAIGQGA
                     VTVTPLQLAEAYAGMLEGGEWRRPHLVPWDEMQAIRPGFVPPEPRRFPLDHENVARIA
                     SGLWAVVNEAGGTGVRARIPGYDVIGKTGSAQVASNQFTKGKKDEELKDNAWFVGAVP
                     KDNPEIVVVAVAQHAEHGYLAAPIVRDVLKAYLDKQIRRQMTRQEEKPAVETGELRFV
                     TPPVPN"
     gene            5880..6965
                     /gene="rodA"
                     /locus_tag="VFA54_03635"
     CDS             5880..6965
                     /gene="rodA"
                     /locus_tag="VFA54_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZS49926.1"
                     /translation="MRHSIRDLDWTLLGFVLAIATLGVLQIYSATRNTIWQGAQNRQI
                     AYIIVGVFLMWLISQVDYHVLLERGPVLYLISIVGLIGIYIVGEVRKGSRRWIPLFGG
                     FNFQVSEFVKLVLILLVARYFSQLNRETVSGVDLLKIGGLVGVPMLLVMKQPDLGTAL
                     TYLPIAAGGVLLAGVRWKHLAIVVILGALVLPAGWHFLKPYQKERLVTFVDPNRDPRG
                     SGYQLIQSRIAVGAGGLWGKGIAKGSQTQLRFLPVPHTDFIFSAFAEEHGFTGVLLAL
                     ALYFVVLMRIIYDAQTAADRAGTYICVCVCVLLLFHLLVNVGMVIGRMPVTGIPLPLM
                     SYGGSSMFAVFLMLGLVNNVWLRRFVN"
     gene            7354..10005
                     /locus_tag="VFA54_03640"
     CDS             7354..10005
                     /locus_tag="VFA54_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZS49927.1"
                     /translation="MSKELIVSSTAHETKVAILEDDELVEIYFEREKQFGLAGSIYKG
                     RVTRVLPGMQSAFVNVGLDRDAFLYVSDFFEDNEEYDRIVTTVEDKVVKMEEKKEALA
                     QAALGEARQEPENGGPAAQEPESSTYEVRSEKHPSSAQAAAPNGHTGAPALFGSGHRG
                     RRGRRRRMGSGRGFPETKFIQPAEVATEAAEASPGPEVTFEVLPGESLAKYRQAAESA
                     GLEAKQDSEQQGLSAQHEGDMDAPSHHLLAREALAEDSRESMDSKAAYHALAAESPSE
                     NGRFSGEQEAAPLPGAVLAAPHGAASEAIAGTAREPYRDEEVIPPQVGLSDEAAPEPD
                     AGEESFSPSESTLEREVLESEHGRQEGLESVGVYSSLSQKDASGSLEEISAEQVGAEA
                     LQTGGVAEGLAGPRPEAAGEAAAAPEAIVEGASEREASVREKPYGQRYLHRGSRRMRR
                     RGREFRDRRGAERSAETSQAPTAEAKLATPRTAETSITDLLKEGQEIIVQIAKEPIGK
                     KGARITSHIALPGRYLVYMPTVDHVGVSRKIVSDEERLRLKRIVQTHRVGMPGGFIVR
                     TAGEGQPEEEIRSDMHFLYNLWLDLRQKAERTPAPALIHHDLNLVERLLRDQLTDTFK
                     TIWVDTQEDYERILQFVERFQPNLVGRVKLYTRSTPIFDEFGITPELEKALRPKVWLK
                     SGGYIVINQTEALVAIDVNTGKYVGKSNRLEDTIVKTNMDAVKEIVRQIRLRDLGGII
                     VVDFIDMDERKNRQKVMQALDEALRADRSPSKILQFNDFGLVAITRKRVKQSLERTLC
                     SPCPYCDGSGYVKSPATVIQEILAEAKKIAPGLDQGNVTLRVNQEVAKVLKSRDNSYL
                     EELETILRRPVLVKGDPLLHQEKFDFN"
     gene            10127..10483
                     /locus_tag="VFA54_03645"
     CDS             10127..10483
                     /locus_tag="VFA54_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZS49928.1"
                     /translation="MPTTPRGSNPGLTLETCADKDATVVHCAGKLTAASTSIFHERVK
                     GLFPTAKRVVLDLTDIAQMDSMGLGTIVGLYVSAKASGCELELINLSKQVRRLLSLTN
                     LLSLFEPCGQHMTRIP"
     gene            10600..>11399
                     /locus_tag="VFA54_03650"
     CDS             10600..>11399
                     /locus_tag="VFA54_03650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS49929.1"
                     /translation="MWNSRGAAGCVIAALIFFSALRCYKTYRVFNDTADESSHIGAGL
                     DILQTGSYATDYVQPPLARIALALPAYWMGLRRSAQERKLSHHALWTRAPRAFYWKTL
                     AAARAGNLIFLPFLIAYVFVWGKQLYGPVAGLAAASATSFSPNLLGHASLATVDFGAA
                     AAIFIAAYYLWRWAQQTGARHCIAAAAACGIAVLVKFSALFFLPPLGIAFFLLAAQEQ
                     HRAGGPPQIAAMARTGALFLAVLIFVAWAGYRFETGPLPPARIQPVPGT"
BASE COUNT         2251 a         3209 c         3599 g         2340 t
ORIGIN      
        1 tgccgttggt gtagatgacg gggaagttca gctggtcttc ggtggcgtcg agatcgatga
       61 aaagatcgta gatttcgtcg agcacttcgc tggggcgggc gtcggggcga tcgatcttgt
      121 taattactac gatcagggcc aaacgcgcct cgagcgcttt ccggagcacg tagcgggttt
      181 gcggcagcgg tccctcgctg gcatcgacca gcagcattgc gccatccacc agcttcaggg
      241 cccgctccac ctcgccgccg aaatcgctgt ggcccggggt atcgacgata ttgatcttga
      301 cgccccggta aaaaatggcg gtatttttgg cgagaatcgt gatgccgcgc tccctttcga
      361 ggtcattcga atccaggact ctctcgacca cttgctcatt ggcgcggaaa atgccgcttt
      421 ggtgcagcat ggcatccacc agggtggtct tgccgtgatc cacgtgggcg atgatggcga
      481 cgtttcgaaa ggaagaaatc aaatatcatc atgacacgat tcgttcgcga aaaccctctg
      541 cccggctgct ccgccctccc gagtttggcg aagctccggc agccatgagc attgccgcct
      601 gtctcatata gcccgccgag gctttccgcg ccgggcgcag ccggtctcag caaagtcctt
      661 cgccggagag gccgtggcgg cgggcttccc ggccccttgg ccggtgcatc taacaccttg
      721 aaaatacaga agaatatcct atctgccgct tgtggcagcc ctccgggcgt ggtaccctga
      781 aattgctggc ctggcaatgg cgagcagttc cgcgtcccag ccgaatgttt ccgcccattc
      841 cggcgctgag ttgcggcggt gcggggtgtg cttaggagcc ccgccgagcc gacgcggcgc
      901 gaccggcgaa gtccagcgtg tacccgacat gaaggagtca tgtcccccag catgaacctg
      961 cgctctctgt tcagtgtatt ttccagcgac cttgccatcg atttaggaac tgccaatacc
     1021 ttggttttcg cccgcggcaa gggcatcgtg gtggatgagc cttcgattgt cgccatcaac
     1081 aagaataccg gcgatatcga ggcggtgggc aaggaggcca aggagatgct cggccgcact
     1141 cccggcaaca tcgtcgccat acggcccatg aaggacggcg tgatcgccga tttcaaggtc
     1201 accgagaaga tgctgaacta tttcatccag aaggcgcaca accgcaaaat gctagtgcac
     1261 ccccggatcg tcatcggagt gcccagcgaa atcacgcagg ttgaaaagcg cgctgtcatg
     1321 gattcggcct atcgggcgca ggccagcgag gtgcatctgg tcgagcaggc catggtggcg
     1381 gccatcggag cagggctgcc gattacggaa ccctcgggca atatggtcgt ggatatcggg
     1441 ggcgggacca ccgatgtcgc ggtgatctcg atgtcgggca ttgtctattc gcggtcggtt
     1501 cgaatcgccg gcaacgagat ggacgaggcc atcatgcagt acctgaagcg caaatacaac
     1561 ctgctcattg gcgagcgcac cgccgagcag gtcaagatcg aaatcgggtc tgcctatccc
     1621 ttggataagc cgttgaccat ggaaattaaa gggcgcaacc tcatagaggg ggtgcccaag
     1681 acggtcactg tggatgacag cgagatccgg gaatctcttt cggagtgcgt cgctaccatc
     1741 atgaacgcga tccgcgtggc gctcgagcgc acgccgccgg agttgagcgc cgatatcagc
     1801 gaccgcggca tcgttctgac cgggggcggc gccttgttgc ggaacctgga taagcgcatc
     1861 cgcgaagaga cgggattgcc cgtgtctatc gcggaaaatc ccctggcgtc ggtggtgctc
     1921 ggcaccggca agatgctgag cgatttcaag ctgcttagga aaatttcgat tgattagcag
     1981 atgcgtgcgg atgagaacgg attagacggc tgtctggatg tggttttcgc ggcctgccgc
     2041 tttggtgtta ggttgaaggc gctgaggttg gcggaggagg accgccgtct ggcgggagcg
     2101 ccttgccggc gcacggacgc cgtcccgata gcagtctctg cggttcgcac ataacaacag
     2161 cgatggagac gttactcgct cggtaccgca acattacggt tctggcgctt gccatctttg
     2221 cccaattgct tttgctgggg taccagatcc ggagcgaccg ggacgtccgg ctgatccgcg
     2281 tgtggggcgt cactgcgatt acaccggtgg cccgagtagt gcatggcgtg cacgagtccg
     2341 ttgcggctct ctggtcaaac tatatttggc tgcgcggcgc caggcagcaa aacgagcgcc
     2401 tgcgcgccga aatcggccgt ctgaaactgg aaaatcaggc tctccgcaac agcctggaag
     2461 ccgccgagcg cctcaagatc ctgacggcgt atcaacaaaa catcctgtcc gagactacgg
     2521 tggcctcggt tatcggagcg ggcgccaatc ccaattcacg tgtaatcttt ctcgacaaag
     2581 gagccggttc aggggtgaag accggcatgg ctgtgatcac tcccgacggc attgtcggcc
     2641 gggtccaatc cgtgtttccc ggatcttcgc ttgtgcagtt gatcgacgat acgaattccg
     2701 ctgtcggagt gattctcgaa accagccgcg cccatggcat tctcaagggc gctggcctga
     2761 atgaagggcg tatcgactac gtgcccaatg aggaaaaagt cgacccggga gagagagtct
     2821 atacgtcggg tgaagacaga atttatccca aagggctgtt ggcgggaata gtgctcgaat
     2881 gccgtcaggg ccgcgatttc aaagagatca ccgtccgctc tgccgctcgc ctggatcgtc
     2941 tggaagaggt gctggtggta acgcgcggcg tgcatcagga cctgccccgg aaggttccaa
     3001 aacccggagc gccggaagtt ttgctgccac ccccgccgat cgaggaccgc gacggcctct
     3061 ccggcctgag cgccccgagg acggccgtgg caggcggcgc aggcccggcg ggttctgccc
     3121 cgagcctgcc ggcggacggt tcggtcgcgg ggaccgatgc cgaccgggta aaacggcggt
     3181 atgaagaatt aggcgccatg caaggacaca agtttggaga aggcgccccc ggttccaagc
     3241 cgcctgattt taatctcggc tggagcccgc aaggcggcat ggcgctccgg cggcccccgg
     3301 ctgccaagcc accgggcccc agtggatccg ccgatgcgcc tggcagcggc gccggaagcc
     3361 tggcgccgcg cgttcccgga acgaatgctc cgcctcagcc gccggacaaa cctggcgcag
     3421 cccccggatc ggtaaggccg taacatgcaa gccagcgtca agcaactggt cgaagcagat
     3481 atcaaccggc tgcgcccctg gattgccatt gtggccgtgc tgggggcatt actgctgcaa
     3541 gcctacttgc ccctgctgtt gccgctgttc tccagctacg ccaatatggc ggacctgccg
     3601 ttgctggtca ccatctatct ggccctgctg cggcggagtc ccgtggctgg tttgtcgatt
     3661 ggcatgggcg tgggcctggc tcaggacagc ctttctgacg gccctatcgg catgtacgga
     3721 atcattaaaa cgattatcgg ttatatctgc tcctcgctga cgcaagtcat tgcggtcgag
     3781 ccgctgggca cccgcgtcat cctggttttc ggctgttatt tgattcacca agctgtcttc
     3841 tgggtcatgg agcgggtttt gcttggccag gcggtcgctt tcctgtggca gagggccttg
     3901 ttcctggcgg taatcaacgc cctgcttgct ttgctgctgt ttcggttctt ggataaattc
     3961 cgggagagga tctgatccgt ggcgacgctg aatccgcttg atccccgcaa agcccagttt
     4021 gcaaaatttg cccaggggcg aattgcgctg attcagtacc tttgcttgtt tgtgttcctt
     4081 tacctgatct caggtttttg ggaacttcag gtgcaaaatc cggatgtata cgccgaacag
     4141 gctgagcgga atcgcgtaaa atggctgccc ttgttggcgc cgcggggcaa aatcctcgac
     4201 cgtgaaaacc gggtgctggc ggataaccat tccaccttca gcgtgattct ttcgcggagc
     4261 aactttgatg aaaagcatct gcctgtgatc gccgacggcc tgaacgtttc cctgccggat
     4321 ttgcaagccc ggttgcgccg gtcgcggaat gagccggaat atgaagccct ggtaatcaaa
     4381 aacggcctca ccgaagctga ggtggcgttc gtagaggccc atcatcagga actgcccgaa
     4441 ctcgaattag ttcgctcgca gcggcggctc tacccggcgg acggttttgc ctcacacctg
     4501 ctcggatacg tgggcgaggt cagcgaagag gaattgaacc agccggaatt tgcgatgtac
     4561 aacccgggtg ccgtcgtggg caaggcggga atcgagaggg agtacaacga cgtgcttacc
     4621 ggcatcgatg gccagcgcca ggtcatagtg gacagccgcg ggcgcgagcg gggcgtcatt
     4681 gggattaaag aggccaagcc gggccgcagc atccggctca ccattgacct cgacttgcag
     4741 gcggctgcgg aattggcgat gcaaggcaga acaggcgccg tggtggcttt ggacccgcgt
     4801 accggagagg tgctcgccct ggtcagcaca cccaattacg acccgaataa gttcgcgggc
     4861 catatcagcg ctgccgattg gaaggaattg gtgtccaatc cggataaccc attgctcaac
     4921 cgggcgatcc aggctcaact ggcgcccggc tctacgttca agccctttgt tgccctggct
     4981 gggctggaaa gcaagacgat cgacgaagac ttttcggtgc actgcggcgg cggagcgtcc
     5041 ttctacgggc gctactttaa gtgctgggtg aagaccggcc atggggctgt ttctctgcac
     5101 aaagccatcc gcgaatcgtg cgacgtctat ttctacaacg tcggcaataa aacagggatc
     5161 gacaccatcg cccaatacgc ggaagcggca ggcttcggcc gccgtaccgg cattgacctg
     5221 ccggaagaga aagacggcgt gatgccttcc tcgaaatgga agcttcgtaa cttccggcaa
     5281 aagtggtatg cgggcgagac gatttcggtg gcgatcggac agggcgccgt gactgttacg
     5341 cctctccaat tggcggaggc gtacgccgga atgctggagg gaggggagtg gcgccgcccg
     5401 cacttggtgc catgggacga aatgcaggcg atccgccctg gattcgtccc gcctgagcca
     5461 cggcggtttc cgctcgatca cgagaatgtg gccagaatcg cttccggatt gtgggctgtt
     5521 gtgaatgagg cggggggaac gggcgtgcgg gcgcgaattc cgggctacga cgtaatcggc
     5581 aaaaccggat cggcccaggt tgcttccaac caattcacaa aagggaagaa ggatgaagag
     5641 ctcaaagaca acgcgtggtt tgtgggagcc gttcccaagg acaatcccga gattgtggtg
     5701 gtggcagtgg cgcaacacgc cgagcacggc tatctggcgg ctcctatcgt ccgggacgtg
     5761 ctgaaggcct atctcgacaa gcagattcgc cgccagatga cccggcagga ggaaaagcct
     5821 gcggtggaaa ccggcgaatt gcggttcgtc acccctcccg tcccgaatta acgtttgcta
     5881 tgcgtcactc gatccgagac ctagattgga ctttgctggg gttcgtactg gcgatcgcca
     5941 cgctgggggt attgcagatt tacagcgcta cacgaaacac catctggcaa ggggcccaga
     6001 atcgccaaat tgcgtacatt atcgttggcg ttttcctgat gtggctgatc tcccaagtgg
     6061 attatcacgt actgctcgaa cgcggaccgg tgctgtacct gatttccatt gtgggcttga
     6121 tcggcatcta tatagtaggc gaagtccgaa agggatcccg gcgctggatt ccgttgtttg
     6181 gcgggttcaa ttttcaggtt tcggaatttg tcaagttggt gttaatattg ttagtcgcgc
     6241 gctacttttc gcagttgaac cgcgagacgg tttccggcgt tgacttactg aaaataggcg
     6301 ggttagtagg tgttccgatg ctgcttgtga tgaagcagcc ggatctggga acggcgctga
     6361 cgtatttgcc gattgctgcg ggcggggtgc tgctcgccgg ggtccggtgg aagcatctgg
     6421 cgattgtagt gattcttgga gctttggtgc tgcccgccgg ctggcacttt ctcaagccgt
     6481 accaaaagga gcggctggtg acatttgtgg accccaaccg ggacccgagg ggctcgggat
     6541 accagctcat tcagtccaga atcgcagtcg gcgcaggggg gctctggggc aagggcatcg
     6601 ccaagggatc tcaaacccag ttgcggtttt tgccggtacc ccatacagat tttatctttt
     6661 cggccttcgc tgaggagcat ggctttaccg gcgtgttgct ggcgctggcc ttgtactttg
     6721 tggttctgat gcgcatcatt tacgatgcgc agacagcggc ggatcgcgca gggacttaca
     6781 tttgcgtgtg cgtctgcgta ctgctgttgt ttcatttgct ggtgaacgtg ggaatggtga
     6841 ttggtcgaat gccggtcaca ggaattccct tgcctcttat gagctatggc gggtccagca
     6901 tgttcgcggt ctttttgatg ctgggactcg tcaataatgt ttggctccgg cgatttgtga
     6961 attgactggt tccacgaacc ggggaggcat acgaagtcag cccgggaggg accaggtatt
     7021 tttaggcggc cgttagagaa gttgcggcag ccgtcaaaat tgaaaaggat aaacgggaac
     7081 ccgagaattc tcgcctgaag ggccggataa ggagtccggg cggaggcaga gagcggttcc
     7141 agcggccccg gggcgttgag cgcaagattg ggaaacgggc tccgccctgc ggtctgagga
     7201 aaatcggtat ctgtttcgag tgcattcccg gccggaataa ggcccggctg atggatgcac
     7261 cgcagcggcg acgatgccgc cgtctccatc cgtcaatccc gcctgattat tgccgagagg
     7321 gcgtcctcgg gctcccttaa ctgaggcatt tccatgtcga aagaattgat cgtgtcctcg
     7381 acggctcatg aaaccaaggt ggccatcctc gaagatgacg agctcgtcga gatctacttt
     7441 gagagagaaa agcagtttgg attggcgggc agcatctaca aggggcgcgt tacccgcgta
     7501 ctgcccggca tgcagtcggc ctttgtgaat gtgggcctgg accgtgacgc tttcctctac
     7561 gtttctgatt tttttgagga caacgaagaa tatgaccgga tcgtgacgac tgtggaagac
     7621 aaggtcgtca agatggaaga gaagaaagaa gccctggctc aggcggcgct cggggaggcg
     7681 cggcaggagc cggaaaacgg cggaccggca gcgcaagagc cggaatcttc cacatacgag
     7741 gtccgttcgg agaagcatcc aagctccgct caggcagcgg ctcccaacgg gcataccggg
     7801 gcgcccgcct tgtttggctc agggcataga ggccgccgtg gacgccgccg gcgaatgggc
     7861 tcgggccgcg gttttccgga gacgaagttt atccagcccg ctgaggttgc caccgaagcg
     7921 gcggaggcct cgcctggtcc tgaagttacc ttcgaggtgc ttccaggcga atcccttgcc
     7981 aagtatcggc aggcagccga atcggccggc ctggaggcca aacaagactc ggagcagcaa
     8041 ggtctttcag cccagcacga aggtgacatg gatgctccct ctcatcatct gctggcgagg
     8101 gaagccttgg cggaggattc cagggaaagc atggattcga aggcagcgta ccacgctctt
     8161 gctgctgaga gcccttcaga aaacggccgc ttttccggcg agcaggaggc cgctccgctg
     8221 cccggggcgg tcttagccgc ccctcacggg gcagcctcag aagcgattgc cggaaccgct
     8281 cgcgagccgt atcgggacga agaagtgata ccaccgcagg tcggattgtc cgatgaggca
     8341 gcgcctgagc cggatgccgg cgaggagagt ttttccccct cggaatccac gctcgaacgt
     8401 gaagttttgg aaagcgaaca tggacgccaa gagggcttgg aatccgtcgg cgtctacagc
     8461 agcctttccc agaaggatgc cagtggatct ttagaagaga tttccgccga gcaggttggg
     8521 gcagaggctc tgcaaaccgg cggcgtggcc gagggtttgg ccgggcctcg cccggaggcg
     8581 gcaggcgagg ccgccgcggc gccggaagct atcgtggaag gcgcttccga acgtgaagcg
     8641 agcgtccgcg agaagccgta tgggcagcgg tatctacacc gtgggtcgcg gcggatgcgg
     8701 cggcgcggcc gtgagtttcg ggaccgccgc ggggccgagc ggagcgccga aactagccag
     8761 gcgccgaccg ccgaagcgaa actggccaca ccgcggaccg ccgagaccag catcacggat
     8821 ctgctcaaag agggtcagga gatcattgtt cagattgcca aggagcctat cggcaaaaaa
     8881 ggcgcgcgaa tcacgtctca tattgcgctc cctggccgat atctggtgta tatgccgacc
     8941 gtcgatcatg tcggcgtctc cagaaagatc gtttccgacg aagagaggct ccggctgaaa
     9001 cgcattgtgc agacccatcg cgtcggcatg cccggtggct ttatcgtgcg aaccgccgga
     9061 gagggccagc cggaggaaga aattcgctcg gatatgcact tcctgtacaa cttgtggctc
     9121 gacttgcggc agaaggcgga gagaaccccc gcccccgctt tgattcacca cgatctcaac
     9181 ctggtggagc ggctgctgcg agatcagttg accgacacct tcaagaccat ttgggtggac
     9241 acccaggaag attacgagcg gattctccag tttgtagagc gcttccagcc caatttagtc
     9301 ggccgcgtca agctctatac gcggtcgacg ccgatttttg acgaattcgg catcacgccg
     9361 gagctcgaga aagcgttgcg gcccaaagta tggctgaagt cgggcggcta tatcgtcatc
     9421 aaccagaccg aagccctggt ggcgatcgat gtgaataccg gcaaatatgt tggtaaatcg
     9481 aaccgcctcg aagacacgat tgtaaaaacc aatatggatg cggtgaagga aatcgtgcgc
     9541 caaatccggc tgcgggacct cggcggcatt atcgtggtcg attttatcga tatggacgag
     9601 cgcaagaacc gtcaaaaagt gatgcaagcg ctcgatgagg cgcttcgcgc ggatcgctcg
     9661 ccgtccaaaa ttctccagtt caatgatttc ggcttggtcg caattactcg taagcgcgtc
     9721 aaacaatcgc tggagcgcac tctgtgctct ccttgcccgt actgcgacgg ctccggatat
     9781 gtgaaatcgc cggccaccgt cattcaggag attctggcgg aagcgaagaa gatcgctccg
     9841 gggctggatc agggcaacgt caccctgcgc gtgaaccaag aggtagcaaa ggtgctgaag
     9901 tcccgggata acagctacct cgaagagctg gaaaccatcc tgcggcggcc ggtgctggtg
     9961 aagggcgacc ctctgctaca ccaggagaag tttgacttca actaaaattt ctccgcgcgc
    10021 cgccggagcg gcagcgtggc ggcttcccgc ccggcaggcc tgattcccgg gcagggaagt
    10081 caagaaaatc gccggagatg cagttgccgg aaggggcaaa agcgcgatgc cgactacacc
    10141 gaggggttcc aatccggggc tgacgttgga aacctgcgcc gataaagacg ccacggtcgt
    10201 gcattgcgcc ggtaagctga cagccgcatc cacctcgatt tttcacgagc gcgtcaaagg
    10261 actgttcccg acggcgaagc gggtcgtcct ggacctgacc gacatcgcgc agatggacag
    10321 catgggcctt ggcacgatcg taggtcttta tgtttcggcg aaggcctcag gctgcgagct
    10381 ggaactcatc aatctcagca aacaggtgcg gcgtctgctg agtctgacga atttgctttc
    10441 gttgttcgaa ccctgcggcc agcacatgac ccgaattccg taaagcctac cgccttgcgc
    10501 gctgcggaat tttcgcgaag ggctgcttgt ttcgcttgcc cgaaggcttc gcttgccgct
    10561 ccggaacgat tccggtaagc tcgtctttcc ccttcttcta tgtggaactc gcgaggggct
    10621 gccgggtgcg tgattgcggc gctcattttt ttctccgctt tgcgctgcta taaaacctac
    10681 cgggtcttta acgacacagc ggatgaatcc tcgcacatcg gggcgggcct cgatattttg
    10741 cagaccggaa gttatgcgac ggattacgta cagccgccgc ttgcccggat cgcgctggcc
    10801 ctgccggctt actggatggg tctgcggcgt tcagcccagg agcggaaact gagccatcac
    10861 gcgctttgga ctcgcgctcc gcgcgcattc tattggaaaa ccttggcggc ggccagagct
    10921 ggcaatctga tctttttgcc gtttttgatc gcttatgttt tcgtttgggg aaagcaactc
    10981 tacgggccgg tagcgggcct ggccgccgcg tcggccacaa gtttttcgcc caacctgctg
    11041 gggcacgcct cgctggctac ggtcgatttc ggggctgccg cggcgatctt catagcggcc
    11101 tattacctct ggcgatgggc gcaacagaca ggagcgcgcc attgtatcgc agccgccgca
    11161 gcttgcggaa tcgccgtgct ggtcaagttt tccgccctat ttttcctgcc gccccttggc
    11221 attgcgttct ttctgctggc agcgcaagag cagcatcggg ccggcgggcc cccgcagatt
    11281 gccgccatgg cgcgcactgg cgcactcttt ttggcagtct tgatttttgt ggcctgggcc
    11341 ggctatcggt tcgaaacagg acctttgccg ccggcccgaa ttcagcctgt gccgggaac
//
LOCUS       DASREK010000065         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_202272, whole genome shotgun sequence.
ACCESSION   DASREK010000065 DASREK010000000
VERSION     DASREK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202272"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFA54_03655"
     CDS             <1..271
                     /locus_tag="VFA54_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49930.1"
                     /translation="VRSRGGWAQFTFVATPRLSFNLYGGEDDPNNRDLAAGGIARNLS
                     YAANVFYKLAPNVVVGGEVSQVRTQFLNGQRPLNNHYDLAVAYLF"
     gene            282..989
                     /locus_tag="VFA54_03660"
     CDS             282..989
                     /locus_tag="VFA54_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49931.1"
                     /translation="MRTAALMLMWTAGCCAATLTGSVVLVNSEQASVKNRHDYSGVVV
                     WLERAGGTPLRASQGFRHAKMEQRNKTFVPHVLAVEVGTAVDFPNLDPIFHNAFSNYD
                     GQVFDVHLYAPQTSRRVVFRRPGMVRVFCNIHATMSAVIAVLPTPYFAVTGPDGRFKI
                     EAPEGAYRFQVWHERSNAETLSRLERPIQLGPRGLVLAELRVSEQGYLALPHKNKYGH
                     DYPPAPEDHVFYPLGPQ"
     gene            993..2768
                     /locus_tag="VFA54_03665"
     CDS             993..2768
                     /locus_tag="VFA54_03665"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS49932.1"
                     /translation="MRLLTKIWLSTSVAITALFAATGWIVSQHLVTEIMKQLTAEVAT
                     SLQAYRSLWRERAQMLETLSAQIASSPQVRAAFGTGDAATIADTSGDLLRNLERQLGE
                     RGLVVVTDPQGRVIARLAAYQGVAAPGRLDFVNDARSKFPAQQSGFDVIEDHLYQFVV
                     TPVYVQSGGGLALIDVLVTGFEVTDAVARELRLSTGSDFVFRSDGKTFASTLGPARAR
                     LLPLVGGRPAGQAQPISAAGLHYIELSEPLLNVEGEPAGSLSVLRSLEAAAAAASALR
                     RQIAAIWVVSLVLSFVITYILARRIIQPIEALDYAAAQVARENYDYRVEAVSNDEIGR
                     LAQTFNHMCASIRQARQELIRNERLYTIGRLASSIVHDLRNPLAAIYGGSEMLLHADL
                     PAPQQKRLSANLYKASQRIQQLLDDLLNVGRGNTGGAREERPLGEILQGAVEPLRNAA
                     EAQKVEIHVSGAEDLVVAADRSRLERAFSNLLVNALEVMPGGGRISIDCRADGENIVI
                     RVADTGPGIPAEIRDRLFQPFASAGKKNGTGLGLALARQTVLDHGGKMWADSGAGPGA
                     AFFIVLPLARPGARFSANLEGQRVV"
BASE COUNT          492 a          896 c          922 g          567 t
ORIGIN      
        1 cgtgcgatcc agaggcggct gggcgcaatt taccttcgtc gctacaccgc gcttgagctt
       61 caatttgtac gggggggaag atgacccgaa caaccgggat ctggccgccg ggggaatcgc
      121 ccgaaatctt tcctacgccg cgaatgtgtt ttataagctg gctcccaatg tggtggtagg
      181 aggggaggtt tcgcaagttc gtacgcaatt tctcaacggg cagcggccgc tcaacaacca
      241 ttacgatctg gccgtggcat atttgttcta ggatctgacg tatgcggacc gccgcgctga
      301 tgctgatgtg gaccgccgga tgttgcgccg ccaccttgac cggaagcgtg gtcctggtca
      361 attccgagca agcgtcggtg aagaaccggc acgactattc aggcgtggtc gtctggctcg
      421 aacgcgccgg tggaacgccc ctccgggcga gccagggctt ccggcacgcc aaaatggaac
      481 agcgaaacaa aaccttcgtt ccccacgtgc tcgcggtgga ggtcggcacg gccgtggatt
      541 tcccgaatct ggacccgata tttcacaacg ccttttccaa ctatgacggc caggtgttcg
      601 atgttcatct ttatgcgccg cagacctccc ggcgcgtggt cttccggcgg ccgggcatgg
      661 tgcgcgtgtt ttgcaacatt catgcgacta tgagcgcggt gatcgccgtg cttcccacgc
      721 cctattttgc cgtcaccggc cccgatggcc ggttcaagat cgaggcgccg gaaggcgctt
      781 atcgcttcca ggtgtggcac gaacgcagca atgcggagac tctgtcgcgc ctggaacggc
      841 cgatccaact tggccctcgg ggcctggtcc ttgccgaact gcgggtttcc gagcaggggt
      901 atttggctct gccgcacaaa aacaagtatg ggcacgacta tcccccggct ccggaggacc
      961 atgtattcta tccgctgggc ccccagtagc tgatgcgcct gcttaccaaa atctggctct
     1021 ccacgtcggt ggcgattacg gctcttttcg cggccacggg ctggatcgtc tcccagcatc
     1081 tggtcaccga gatcatgaag caactgacgg ccgaagttgc aacgagcttg caggcctacc
     1141 gctcgctgtg gcgcgagcgt gcccagatgt tggaaaccct gagcgcgcag atcgccagtt
     1201 cgccgcaggt gcgggcggcc ttcggcaccg gggacgccgc gacgattgcc gacacttccg
     1261 gcgatttgct ccgcaatctc gagcgtcagc tcggcgagcg cggcctggtg gtggtgaccg
     1321 atccacaggg gcgcgtaatt gcgcggctcg ccgcttacca aggagtcgcg gcgcccggcc
     1381 gcctggattt cgtgaacgac gcgcgcagca agttcccggc gcagcagagc ggctttgacg
     1441 tcattgagga tcatctgtac cagtttgttg tgacgccggt atatgtgcag tccggcggag
     1501 gcctggcgct gatcgatgtt cttgtaaccg gttttgaagt caccgacgcc gtggcgcggg
     1561 agctgcggct gtcgacgggc agcgattttg tcttccgatc ggacggaaaa actttcgctt
     1621 cgacgctcgg tccggccagg gctcgtttgc tgccccttgt gggaggccgg cccgccggcc
     1681 aggcgcagcc catttccgcc gccgggctgc actacatcga gctttccgag ccgctgctca
     1741 acgtggaagg cgagccggcc ggttcgctga gcgtgctgcg ctcgctggag gcggccgcgg
     1801 ccgccgcttc cgctttgcgc cgccagatcg cagccatctg ggtcgtgagt ctcgttctga
     1861 gttttgtaat tacgtatatt ctggccagac ggatcattca acccatcgaa gcgctggact
     1921 acgccgccgc ccaggtggcg cgcgagaatt acgattaccg cgtcgaagcc gtatccaacg
     1981 acgagatcgg ccggctggcg cagaccttca atcacatgtg cgcctccatc cggcaggcgc
     2041 ggcaggagct gattcgaaac gagcgtcttt acacgatcgg ccggctggcg agctccatcg
     2101 tgcacgacct gcggaatccg ctggcggcca tttacggcgg gagcgaaatg ctgctgcacg
     2161 ccgatttgcc cgctccccag caaaagcgcc tttccgccaa tctctacaag gcgtcccaga
     2221 gaatccagca attgctggac gacttgctga atgttggccg gggaaacact ggcggagctc
     2281 gtgaagagcg ccctctgggg gagatcttgc agggagccgt cgaaccattg cgcaatgccg
     2341 cggaagcgca aaaggtggaa atccacgtgt cgggcgcgga agacctcgtt gtagcggcgg
     2401 atcggagccg cctggagcgc gccttcagca accttctggt caatgccctg gaggtcatgc
     2461 ccggcggcgg ccgcatctcg atcgactgcc gggccgatgg ggaaaacatc gtcattcggg
     2521 tcgcggacac cgggccgggc attcccgccg aaatccggga ccggctcttc cagccctttg
     2581 ccagcgccgg caagaagaat ggcacgggtc tcggcctggc gctggcgcgc cagacggttc
     2641 tggaccacgg cggaaaaatg tgggcggact ccggggcggg gccgggagcg gctttcttca
     2701 tcgttctccc gctcgcccgt cccggcgcgc gattctctgc aaacctggag ggacagcggg
     2761 tcgtgtaagt tcattcgacc ggcccctctc atgtccgccg gcattctcat tttcctggcc
     2821 acctacctgg tgctggcgat tggacgcctg ccgggatttc gcgtggaccg gacgggc
//
LOCUS       DASREK010000066         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2345719, whole genome shotgun sequence.
ACCESSION   DASREK010000066 DASREK010000000
VERSION     DASREK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345719"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VFA54_03670"
     CDS             complement(<1..1113)
                     /locus_tag="VFA54_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS49933.1"
                     /translation="MDQIGLAIIGCGAVVTGRHLPALAAISGAQVCCVYDPDIEAARR
                     VAAGTGARIAAALEEAVQFKDVQAVVIASPNAFHRQGVEAAADAGKHILCEKPLATNL
                     RDARAAQEAARRAGVVLQLGFHHRFTSEFRLVRRLLEAGAIGAVHSFQATMSEPFDVI
                     PGGSANYRLDPKLSGGLTLIDFGSHRIDQLRALAGDAREISACFTSAGGHGLDDNVVL
                     LVAAQSGAIGALSFHRFSRGALAPATLIGKKGVLCFNAYVANPFHAAPVAVFTEEPLP
                     PDVAVFTRPQDWWSPPRSGWIELWPPVVNPFIEEYKAFFGALRSGQISAATGEDGYKA
                     LEIVMAAYKAHHARRAVALPLDPEEVIPLPALTPAAS"
     gene            complement(1120..1911)
                     /locus_tag="VFA54_03675"
     CDS             complement(1120..1911)
                     /locus_tag="VFA54_03675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZS49934.1"
                     /translation="MTSELLRLVSQARIFDLAQPYFVGMPHHPVHPPFLYGLAKKHGD
                     YLGPGGVSSASEALALGSHVGTHIDALCHFSCGGKLHGGIDAASAQSYAGGLRQHSVD
                     TIAPIFRRGVLLDIAGQAGVDALAADFQITPEHLEKAAQAHGVQVEPGNVVLLRTGWA
                     RYWNDAARYIAEVRGPGPQEAGARWLSERRVFAAGSDTVAFERVPAPSMPVHAHLLVE
                     SGIHIIECLNLEELAAEQVYSFLFVAAPLKIRGGTGSPIRPLALA"
BASE COUNT          407 a          667 c          666 g          354 t
ORIGIN      
        1 cgaggcggcc ggtgtaagcg cggggagcgg aatgacttcc tctggatcga gtggaagcgc
       61 gacagcgcgg cgggcatggt gcgccttgta agcggccatc acaatctcca gggctttgta
      121 gccatcctcg ccggtggcgg cggagatttg cccgctccgg agcgctccga aaaacgcctt
      181 gtattcctcg atgaacgggt tcacgaccgg cggccacagc tcgatccatc cggaacgcgg
      241 cgggctccac caatcctggg gcctcgtgaa taccgctaca tcgggcggca gaggctcttc
      301 ggtaaagaca gcgacgggcg cggcgtgaaa aggattggca acataagcat tgaagcatag
      361 aacgcccttt tttccgatca acgtggccgg cgcgagagcg ccccgcgaga agcgatggaa
      421 gctcagagcg ccgatcgctc cgctctgcgc tgccaccagc agcacgacgt tgtcgtcgag
      481 gccgtgcccc ccggcgcttg tgaagcacgc ggagatttca cgggcgtcgc cggccagcgc
      541 ccgcaattgg tcgatacggt gcgagccgaa atcgatcagg gtcagcccgc ctgatagttt
      601 ggggtccagc cggtaattgg cgctgccgcc cggaatcacg tcaaacggct ctgacattgt
      661 ggcctgaaag gagtgtacgg cgccgatcgc gccggcttcc agcaggcggc gcaccagccg
      721 gaactcgctg gtgaaccggt gatgaaatcc taattggagg accacgcccg cccggcgcgc
      781 cgcttcctgc gcagctcgcg cgtcgcgcag gttggtggcg agcggcttct cgcaaagaat
      841 gtgctttccg gcgtccgcgg cggcctcgac gccctggcga tgaaacgcat tcggggaggc
      901 gatgacgacg gcttgaacgt ctttgaattg caccgcttcc tccagagccg ccgcgattct
      961 ggcgccggtc ccggcggcta cgcgccgggc ggcctcaata tccggatcat atacacagca
     1021 gacctgcgcg ccggaaatgg ccgccaaagc gggaagatgc cggcctgtaa cgaccgcccc
     1081 acagccgata atggcgagcc cgatttgatc catgaagact taggcaaggg ccagggggcg
     1141 gatcggcgag ccagtcccgc cgcgaatctt cagcggcgcg gccacgaaaa gaaaggaata
     1201 gacttgctcg gccgccagtt cctcgagatt gaggcactcg atgatgtgga tgccgctctc
     1261 taccagcaaa tgcgcgtgga cgggcatgct tggcgccggc actcgctcga atgcaacggt
     1321 gtcagatccg gcggcgaaca cgcgccgctc gctcagccac cgcgctccag cctcctgcgg
     1381 ccctgggcct cgcacctcgg cgatgtaacg cgcggcatcg ttccaataac gcgcccaccc
     1441 ggtgcgcagc agcaccacat tccccggctc gacctgtact ccatgcgcct gcgcggcttt
     1501 ttccagatgc tcgggcgtaa tctggaagtc agccgcgaga gcgtccactc cagcctgccc
     1561 tgcgatgtcg agcagcacgc cgcgccggaa gatcggagcg atggtgtcca ccgagtgctg
     1621 ccgcaagccg cccgcataag attgtgcgga ggccgcgtcg atcccgccat gcagctttcc
     1681 gccgcaggaa aaatggcaga gcgcgtcgat atgcgttccc acgtggctgc cgagcgccag
     1741 cgcttcggac gcggagctta cgccgccggg gcccaagtag tccccatgct tcttcgctaa
     1801 tccgtagagg aagggaggat gaacaggatg gtgcggcatg cccacgaaat atggctgggc
     1861 cagatcaaag atcctcgctt gcgataccag acggagcagc tcactggtca taagaagcag
     1921 tgactatagc atgaccactc cggcgtttct aatctgaagt tgttgcgcat gtacgggagt
     1981 gcagcgttgt cgatcacaga caagaatttc tgatcagcac gactgctccg agcggctcaa
     2041 gggctgaggc ctgcttctct gagaaattcc gccgcaactt ccgccacagg ccgg
//
LOCUS       DASREK010000067         5612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2345842, whole genome shotgun sequence.
ACCESSION   DASREK010000067 DASREK010000000
VERSION     DASREK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2345842"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFA54_03680"
     CDS             complement(<1..744)
                     /locus_tag="VFA54_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZS49935.1"
                     /translation="MRTLFIGDIVGSAGRRIVADHLGDIVQNQGIDLVIANCENAAAG
                     FGITPRLADELLEMGAHVLTSGNHIWDKREILDYIGRQPRLLRPDNYPEGVPGRGVFV
                     GATATGVDYAVINLQGRTYMPNIECPFRRADAILAALPQSVRLRIIDFHAEVTSEKMA
                     FGWYLDGRATAVVGTHTHVPTADERILPGGTAYLTDVGMTGSYDSVIGADRTAAIERF
                     LYAMPNRLEPAKDNVRLAAVIIDSDEATGR"
     gene            complement(899..1213)
                     /locus_tag="VFA54_03685"
     CDS             complement(899..1213)
                     /locus_tag="VFA54_03685"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZS49936.1"
                     /translation="MEQEGVRVSIFDQVYHLKSGSGDRAYMEQIARYVDQKMAAIASR
                     TQTVDSFRVAVLAALHITDELFRLRQEHEALQESIETKSLEYAALLDEVLETANSAPG
                     GS"
     gene            complement(1241..1525)
                     /locus_tag="VFA54_03690"
     CDS             complement(1241..1525)
                     /locus_tag="VFA54_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49937.1"
                     /translation="MSERSNGAVTDINDLRALSRLEERILATIEELRAARREKTEAEQ
                     ESEALREQLARAEEESRKLSAEIETLRSERKLIGERLERLLSQIDLLGQE"
     gene            complement(1580..3610)
                     /gene="pheT"
                     /locus_tag="VFA54_03695"
     CDS             complement(1580..3610)
                     /gene="pheT"
                     /locus_tag="VFA54_03695"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZS49938.1"
                     /translation="MRIVLSWLAEYIDVKESPRDLAQALTMAGIAVESVSEELGETIF
                     EMDITANRPDAMNHLGVARELAAIFGRQIRKPEIHLREDARPAAACASIAIAAPDLCP
                     RYVGRVAVDVKVGPSPDWMRQRLELCGVRSINNVADITNYVLLEAGHPTHAFDLDTLE
                     GAKIIVRRAEAGERLITLDGVERALLPEHLVIADARRPVALAGIMGGAETEISGRTRN
                     VLIESAWFEPASIRKTARHFGMHTEASHRFERGADIEATTWAAARIAALLQQLASATV
                     LKGAIDAYPAPRQRSAILLRAAAVMRYLGIEIAAVEIESILCSLGFRVERLDGAWQVT
                     PPPARLDVEREIDVIEEIARIYGYERFPARLPAWAGRAEITDDSRKQRQMRDVARALG
                     YDETISLSFLSRSEAAQFGAWEPVAVRNPLSELQSVMRNSAVPGLLRAVEWNLNRGQT
                     DVRLFEIGRLYRQEGSEFAELPVMGLVATGRARPASLNDPGKLYDFFEMKADVAKLLD
                     LFGWKNLYFDRETGAGYYHPGRSARAVADGYAAGRFGELSPEVAAERKLRQPVWLAEI
                     CLDRLFRLPLREPRFRPLPRVPAVDRDFSLLLPEGTRFEDIVAAVGAREHLVRLEPVE
                     IFRGGQAPPGKMSLLLRAVWQRDDASLTDEEVNQYAREILDALRKLGAEQRA"
     gene            complement(3610..4686)
                     /gene="pheS"
                     /locus_tag="VFA54_03700"
     CDS             complement(3610..4686)
                     /gene="pheS"
                     /locus_tag="VFA54_03700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZS49939.1"
                     /translation="MSITEKATRTLAELAADSAAIEGLYQELRELFERELADARDPEH
                     LKKLRDRWLGRKNGLLSFTNDHWLKPASREWKPLVGKLQNSLKSAVEKAIQEAEAARA
                     QPVRSEPAVDVTLPGPPRIIGARHPIRIVQEDIESIFGSMGYSIAEGPEIESDYYNFE
                     ALNFPENHPARDAMDTIFLTDHALLRTHTSPNQVRIMERQPPPLRYLVPGKVYRNDAP
                     DASHSPMFHQVEGFAVDENITFCDLKGTLEHFLKQFFGSNIRTRFRPSFFPFTEPSAE
                     VDISCHVCGGAGCRVCKQSGFIEVLGCGMIDPAVYRYVGYDPERYSGFAFGMGLDRIA
                     MLKYGINDIQLFFQGDVRFLRQFR"
     gene            complement(4704..5066)
                     /gene="rplT"
                     /locus_tag="VFA54_03705"
     CDS             complement(4704..5066)
                     /gene="rplT"
                     /locus_tag="VFA54_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZS49940.1"
                     /translation="MPRVKRGTKRRDRRKKILHSASGYFLTKGKLYRAAKEAVERGLK
                     FAYIGRRQKKRDFRSLWIIRIGAAARANDLSYNKFMHGLKLAGVEIDRKMLADLAIGD
                     AAAFAALAQKAKAALASA"
     gene            complement(5248..5439)
                     /gene="rpmI"
                     /locus_tag="VFA54_03710"
     CDS             complement(5248..5439)
                     /gene="rpmI"
                     /locus_tag="VFA54_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZS49941.1"
                     /translation="MPKLKTHRGAAKRFKRTASGKILRHHASARHILTTKSRKRKRKL
                     RKVALVSAGDHRRIDRMLL"
BASE COUNT         1073 a         1772 c         1662 g         1105 t
ORIGIN      
        1 ccggcctgtc gcctcatctg aatcgataat aacagccgca aggcggacat tgtctttcgc
       61 cggctccagc cggttcggca tggcatataa gaaccgctct atcgccgcag ttctatccgc
      121 gccaatcaca gagtcatagg acccggtcat acccacatcc gtcaaatacg cggtaccccc
      181 gggcaaaatc cgctcatcgg ccgtggggac gtgagtgtgg gtgcctacca ccgccgtagc
      241 tctgccgtcc agataccatc cgaaggccat tttttcgctt gttacttccg catggaaatc
      301 gatgattctc aaccggaccg actggggcaa ggcggccagt atggcgtcgg cccggcggaa
      361 ggggcactcg atgttgggca tataggttcg tccctggagg ttgattaccg cgtagtcgac
      421 tccggtcgca gtcgcgccca caaagacgcc ccgccccggg acgccctccg gataattatc
      481 cgggcgaagc aaccgcggct ggcgcccgat atagtccaga atttcccttt tgtcccagat
      541 atgattgccg gaggtcagca catgcgcgcc catctcgagc agctcgtccg caaggcgggg
      601 cgtgatgccg aagcccgcag ccgcattttc gcaattagct atgaccagat cgattccctg
      661 gttctggaca atatctccga gatggtccgc cacaatgcgc cggcccgccg agcccacgat
      721 gtcgccaata aaaagagttc tcaacgcgaa gatttgtgaa cgattctgtc agggggaaaa
      781 caagcccgga acagaggccc gcacgcctgg cgcgagacct aaacgcgtat tctatcgcaa
      841 acgcagaatc gccaatcgcc ggcgtcgcca cacggagtcg ctgaaagccg cggctttatc
      901 aggatccgcc gggggccgaa tttgccgtct cgaggacttc gtccagcagg gccgcgtact
      961 ccaggctctt ggtctcgatg ctttcctgca aggcttcgtg ctcctggcgc agccggaaaa
     1021 gctcatccgt gatatgcagg gcggcaagca cagcgacgcg gaacgaatcg acagtttgag
     1081 tgcgcgatgc gatagcagcc attttctggt ctacgtagcg cgcaatctgt tccatgtaag
     1141 cgcggtcgcc cgatccgctc ttcaggtgat aaacctgatc gaagatggat acgcgtacgc
     1201 cttcctgctc catttggaac cgcccgctct cacccttata ttactcctgg cccagcaagt
     1261 caatctgcga taacaggcgt tccagccgct cgccgatcaa tttacgttca gaccgcagcg
     1321 tttctatttc cgccgacagc ttccgggatt cctcttccgc ccgggcgagt tgctcccgca
     1381 aggcttcgga ttcctgctcc gcttccgtct tttcccgccg ggcggcgcgc aattcttcaa
     1441 tcgtagccag gattcgctcc tccagccggg aaagcgccct caaatcgttg atgtcggtta
     1501 ctgcgccatt cgatctttct gacatagagt cctctttccg ccggatagcg cttcgcgaga
     1561 aagcgcccgc ggtgcgccgc taggcccgct gctcggcgcc gagtttacgc aaagcatcga
     1621 ggatctcgcg cgcatactga ttgacttctt catcggtcag gctggcatcg tcacgctgcc
     1681 agaccgcccg cagcagcagg ctcatcttgc ccggaggcgc ctggccgccg cggaagatct
     1741 cgaccggctc gaggcggacc aggtgctccc gggcgcctac tgccgcgaca atgtcttcaa
     1801 atcgcgtccc ttcaggcagc aacagagaaa aatccctgtc cacagcaggc actcgcggga
     1861 gcggccggaa acgcggctcg cgcagcggca gacggaaaag acgatcgaga cagatttcgg
     1921 cgagccacac cggctgccgc agctttctct ctgcggcgac ttccggactg agctccccga
     1981 atcgtccggc cgcatagccg tcagccacgg cgcgcgcgga acgcccggga tgatagtatc
     2041 ccgccccggt ctcccggtca aagtacagat ttttccagcc aaacaagtcg agaagtttcg
     2101 ccacatccgc cttcatttcg aaaaaatcgt agagcttgcc tggatcgttc aacgaagccg
     2161 gccgcgctcg gcccgtcgcc accagaccca tcacgggcag ctcagcaaat tcactgcctt
     2221 cctgccggta gaggcggccg atttcgaaga gccggacatc tgtttggccg cggttcaagt
     2281 tccattcgac ggcccgcaac aggcctggga cggcggagtt gcgcatcacg ctttgcagct
     2341 cgctcagcgg gtttcgcact gcaaccggct cccatgcgcc gaattgcgcc gcctcggagc
     2401 gcgaaaggaa agacaaactg atggtctcgt cgtagcccag ggcgcgggct acgtctcgca
     2461 tttgccgttg tttcctggaa tcatcggtga tctcggcccg cccggcccag gccggcaggc
     2521 gcgcggggaa acgctcgtag ccgtaaatgc gcgcgatctc ctcaatgaca tcgatctcac
     2581 gctcgacatc caggcgagcc ggcggcggcg tgacttgcca tgctccatcc aggcgttcca
     2641 ctcgaaagcc gagagagcac aaaatggact cgatttcgac agcggcgatt tcgattccga
     2701 gataacgcat gaccgccgcg gcccgcagca aaatggcgga gcgctggcgc ggcgccggat
     2761 aggcgtcaat ggctcccttc agtacggtgg ccgaggccag ttgctggagc agcgcggcaa
     2821 tgcgcgcggc cgcccaggtg gtggcttcaa tgtcggcgcc gcgttcgaag cggtgagacg
     2881 cctcggtatg catcccaaaa tgccgggctg tttttcgtat cgacgccggc tcgaaccagg
     2941 cgctttcaat gagcacattc cgcgtgcgcc cggaaatttc cgtctccgcg ccgcccatga
     3001 tgccggccag agccaccggc cgtctggcgt cggcgataac cagatgctcg gggagaagcg
     3061 cccgctccac cccgtccaga gttatcagcc gctcgccggc ttcagcgcgc cggacgataa
     3121 ttttcgcccc ttccagcgtg tcgagatcga aagcatgcgt cgggtggccc gcctcgagca
     3181 gcacgtaatt ggtgatatcg gcgacgttgt tgattgaacg cacgccgcag agctcgaggc
     3241 gctgccgcat ccagtcaggt gaaggtccca ccttcacgtc caccgccacc cggcccacat
     3301 aacgggggca cagatcgggg gcggcaatcg caatggaagc gcaggcggcg gcaggacggg
     3361 cgtcttcacg aagatgaatc tccggtttgc gaatctggcg gccgaagatc gccgccagtt
     3421 cccgcgccac gccgagatgg ttcatcgcat cgggccggtt ggcggtgata tccatctcaa
     3481 aaatcgtctc tccaagttct tcagacaccg attccacggc gatgcccgcc atggtaagcg
     3541 cctgggcaag atcgcgcggg gattccttga cgtcgatgta ctcggccagc cagctcagaa
     3601 caattctcat tatcggaact gcctcaggaa ccggacatct ccctggaaga acaactggat
     3661 gtcgttgatc ccgtatttga gcatagcgat gcggtccagg cccatgccaa aagcgaaccc
     3721 ggagtatcgc tcggggtcgt agccgacgta ccggtagacg gccggatcaa tcatgccgca
     3781 ccccaaaacc tcgatgaagc cgctctgctt gcagacgcgg cagccagcgc cgccgcaaac
     3841 gtgacaggag atgtccacct cggcgctggg ctccgtgaat ggaaagaagc tggggcggaa
     3901 gcgcgtgcga atgttcgagc cgaaaaactg tttgagaaag tgctcgagcg ttcctttcag
     3961 gtcacagaag gtaatattct catccactgc aaacccttcg acttgatgga acatcggcga
     4021 gtggctggcg tccggcgcat cgttgcggta caccttgcca ggaaccaggt aacgcagcgg
     4081 cggcggctgc ctctccatga tgcgtacctg gttgggcgag gtatgcgtgc gcagcagggc
     4141 atgatcggta aggaaaatgg tgtccatcgc gtcccgcgcc ggatgattct cggggaagtt
     4201 gagcgcctca aaattgtaat agtctgattc gatttccggc ccttccgcga tcgagtagcc
     4261 catggaaccg aaaatcgact cgatgtcctc ctgcacgatg cggatcggat gccgcgcgcc
     4321 gataatgcgc ggcggtccgg gaagggtgac gtcaacggcg ggttcgctgc gtaccggctg
     4381 agcgcgggcg gcctcggcct cctggatggc cttttcaaca gcgctcttca agctgttttg
     4441 cagctttccg accaagggct tccattcgcg ggaagcgggc ttgagccagt ggtcgttcgt
     4501 gaaactgagc aggccgtttt tgcggcccag ccagcggtcg cgaagcttct tgagatgctc
     4561 cggatcgcgg gcgtcagcca gctcacgctc gaagagttcg cgcaattcct ggtacaggcc
     4621 ctcaatcgcc gccgaatcag cggccagctc tgccaaggtt cgtgtggcct tctcggtgat
     4681 cgacatggga ccgcaaggcc gtctcaggcg cttgccaatg cggccttggc cttctgggcc
     4741 agcgcagcaa atgccgcagc atcgccgata gcaagatcgg ccagcatctt gcgatcgatt
     4801 tccactccgg ccagcttcag cccatgcata aatttgttgt aggacaaatc gttcgctctc
     4861 gccgccgcgc caatgcggat gatccagagg gagcgaaagt cccgcttttt ctggcggcgg
     4921 ccgatgtaag cgaatttcag cccgcgttcg acggcttctt tggccgctcg atacagcttg
     4981 cctttggtaa ggaaatagcc ggacgcgcta tggaggattt tcttgcggcg gtcgcggcgc
     5041 ttggttccgc gtttgactct gggcacggtt tgtctccttc gttgattttg ttagcggcga
     5101 cgcaggcacg tgcctcgcgc actgctcgct cgcttgcggc cggcggccgg acggctgccg
     5161 gaatcccttc gcgacaatcc caagttccct gccggcgtcc taccgggtcg aagcgccccg
     5221 gaagccggtg aacaaggcgg cccgggttta cagcagcatg cggtcgatgc gcctgtgatc
     5281 gccggcgctc accagagcca ccttccgcag cttccgtttg cgcttgcggc tcttcgtggt
     5341 caggatatgg cgggccgaag cgtggtggcg caaaattttt cccgaggccg ttctcttaaa
     5401 acgcttggcg gcgccgcggt gcgttttcag cttgggcaaa gcatacctcc tctccacgcg
     5461 gcgcacacac gtacgattcc gctttcccgt tcgctcgctg gagtctgggg ttttgtgcgc
     5521 ggcagtgcag aaagaacctt cagtataaca cagcgagcat tcagtttgac gatgccggcg
     5581 caggagcggc ccggtttcaa tcagcgaaga gg
//
LOCUS       DASREK010000068         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1686991, whole genome shotgun sequence.
ACCESSION   DASREK010000068 DASREK010000000
VERSION     DASREK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686991"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFA54_03715"
     CDS             complement(<1..253)
                     /locus_tag="VFA54_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49942.1"
                     /translation="MTSTRLAVVDLVPTPYQEPLCRSLSQTPGWKVRFFYLQSKDSVR
                     GWSKLHCPYDAVKLPCLTPESLYPVPLVGVVNWGLLGHLR"
     gene            complement(250..1683)
                     /locus_tag="VFA54_03720"
     CDS             complement(250..1683)
                     /locus_tag="VFA54_03720"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS49943.1"
                     /translation="MSATPEVFAPPRLPTLPWASLPVPAFYATLAAASLHLLTSQSLE
                     AALLGSSLALAVLVAPLEYAVGAWAAAFLAAASSELRNEFLLVRWLVLAFSVVALTLR
                     FFLRRRAGSSHLTKFTYLLLAFLGVSGVTTTTTMAPVLTDLKLGAVICVFYLSSRAGS
                     QLVDSYGPSSARKLTWGLLAYSLAFVAVAAPGYLLSPADSTRFFGNPNTWAALLATVL
                     PWVASPLFRRFRQTGVKLGALAGCFLFGCYLLLLSGSRAAMLGVLLAVTVFCLIHANR
                     RIGAAVLLTSVLITARVLTNPDALSNFSRQYLYKHKVRDAQTNLFESRVQPWEAAMAN
                     FEEHPWLGLGFGVTNKGEAAWSAEVGSWYATETGGSFWGTLSQVGALGSAPLFLAILM
                     LLIEAGRFAWKVRDPWLTGVYGSVLALTVNAIFEGWLIAPGNFIGIYFWMQCFFLNAL
                     ICRFRPAPSRSPALSAPPPSAWADRSR"
     gene            complement(1680..>2923)
                     /locus_tag="VFA54_03725"
     CDS             complement(1680..>2923)
                     /locus_tag="VFA54_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS49944.1"
                     /translation="LFLLLAFLVLAFGGVELWALTIFEVAAFALASVWVLRMAFGRVR
                     PVWNICLLPWAALVLWTWCQYAFGWSIHRYRTEEEALKWLALWLLMAIACHVFTDDSI
                     RSRFRLALVWFAFGLCVFGLVQYFTSRNTIYFLLPVPAGKIFGPFIDANHFTVLMELV
                     TPSALLLALRPSEQRPIYIVVANLILASVVVCASRAGIALVGTETAIVLAASVLGRRS
                     RRRSRERRVLLPIAGLAAVAAFFVLVSSSRPLMERFQEDQPYSVRWNVARATWRLFLN
                     RPLTGYGAGTFEEVFPSAATEDLGMVWQHAHNDPVQFAMEWGAAGIVALIGSVALLLR
                     RKWPREVWLTLVLPLLTLLIHSWFDFPLQIPALAATWLVLLAQVEPAARPGESSPSHS
                     SGRRPGRMARASDRVLKPVHR"
BASE COUNT          716 a          888 c          899 g          420 t
ORIGIN      
        1 cgcgcaggtg gccgagcaag ccccaattca cgacgccgac caggggcacc ggatagagag
       61 attccggtgt aagacaagga agctttaccg cgtcgtatgg gcagtggagc ttggaccatc
      121 cgcgtaccga gtccttgctc tgtaggtaga agaatcgaac cttccatccc ggcgtctggc
      181 tcagactgcg acagagcggc tcctgatagg gagtgggaac caggtccacg acggccaggc
      241 gagtggaggt catcggcttc gatccgccca ggcgctgggg ggcggcgcag agagcgcggg
      301 agatcgcgag ggagcgggcc ggaaccggca aattagtgcg ttcaagaaga agcactgcat
      361 ccagaaatag attccgataa aattgcccgg cgcgatcagc cagccttcaa agatcgcatt
      421 caccgtcagg gcaagaacag agccgtacac gccggtcaac cagggatctc tcaccttcca
      481 ggcgaaacgg cccgcttcta tgagcagcat gagaatagcc aaaaacaatg gcgccgagcc
      541 cagagcgccc acttggctca gagtgcccca aaagctgccg cctgtctctg tcgcgtacca
      601 actgcccact tccgcactcc atgcggcttc gcctttgtta gtgacgccga agccgaggcc
      661 caaccatgga tgttcctcga agttggccat tgccgcttcc caaggctgca cgcggctttc
      721 aaagaggttg gtttgagcgt ctcgtacttt gtgtttgtac agatattgcc tcgagaagtt
      781 agaaagggca tcggggttcg taagcacgcg ggctgtaata aggaccgaag tgagcagaac
      841 ggcggcgccg atcctacggt ttgcgtgaat caggcagaac acagtcacgg cgagcagcac
      901 gccgagcata gccgcgcgtg agccgcttag caacagcaga tagcacccga agagaaagca
      961 tccagccaaa gccccaagct tgacgcccgt ttgccggaac cgccggaaca ggggagaggc
     1021 gacccagggc aagactgtgg ctagtagcgc cgcccacgtg ttaggatttc cgaagaaccg
     1081 ggtggaatcc gccggcgaca ggagataacc gggagccgcc accgccacaa aagccagaga
     1141 ataggcaagc aggccccagg tgagcttgcg ggccgagctt ggcccgtaac tgtccacgag
     1201 ctggctgccg gctcgcgacg acaagtagaa aacgcagatc acggcgccga gctttagatc
     1261 ggtgagaacc ggggccatcg tggtggtggt cgtaacgccc gagacgccga ggaacgccag
     1321 cagcaaatag gtaaacttgg tcagatgcga cgagccggcg cgccggcgga gaaagaatcg
     1381 cagcgtcaaa gccaccacgc tgaaggccag gaccagccac cgcacgagca gaaactcgtt
     1441 tctcaattcg ctcgacgcgg cggcaagaaa ggccgcggcc catgctccca cggcgtattc
     1501 caagggcgca accagcacgg ccagcgccag gctggagccc agtagcgccg cttccaggct
     1561 ttgactggtc agcaggtgca gagaggcggc cgccagagtg gcgtaaaagg cgggaaccgg
     1621 cagagaagcc cagggcagcg tgggaagccg cggcggagca aatacctcgg gcgttgcgct
     1681 catcgatgta ccggcttgag aaccctatcg gaagcgcgcg ccatgcggcc ggggcgccgc
     1741 ccggaggaat gcgatggaga ggattcgccc ggccgcgccg ccggctcgac ttgcgcgagc
     1801 agcaccagcc aggttgccgc aagagccggg atttgcagcg gaaaatcaaa ccagctatgt
     1861 attagcaatg taagaagcgg cagcaccaga gtcagccata cttcccgcgg ccatttccgg
     1921 cgcagcagca gcgcgacgct gccaatcaga gcgacgatgc cggcggcgcc ccattccatg
     1981 gcaaattgca cagggtcatt gtgggcgtgt tgccacacca ttcccagatc ttccgtggct
     2041 gcggagggaa acacttcctc gaaggtcccc gctccgtagc ccgtcagagg gcggttcaaa
     2101 aacaaccgcc atgtagcgcg ggcgacattc cagcggacgc tgtaaggctg atcctcctgg
     2161 aagcgttcca tgagaggccg gctgctggaa accaaaacga aaaaagcagc gacagcggcc
     2221 aagccggcga tgggcaacag aaccctcctt tcccggctcc gccttcgaga ccgccgaccc
     2281 agaacgcttg ccgccagcac gatggcggtt tctgtgccca ccagcgcgat gcctgcgcgc
     2341 gatgcgcaga ccacgaccga agccagaatc agattggcga ccacaatgta gatcggcctt
     2401 tgctcggaag gccgcagcgc cagaagcagc gccgagggcg tgaccagttc catcaagacc
     2461 gtgaagtgat tggcgtcgat aaacggtccg aagattttac ccgccgggac tggaagcaag
     2521 aagtaaatcg tgtttcgcga agtgaaatac tgcaccagcc cgaaaacaca gagcccaaag
     2581 gcgaaccaca ccagcgccag acggaaccgg cttcggatgg aatcgtcggt gaatacatga
     2641 catgcaatgg ccatcagaag ccagagcgcg agccacttca acgcctcctc ttccgtgcgg
     2701 taacgatgga tggaccaccc gaacgcatac tggcaccatg tccacaaaac cagcgccgcc
     2761 cagggaagga gacagatatt ccagaccggc cggacacggc cgaaagccat ccgtaggacc
     2821 cagacggacg ccagtgcaaa cgccgctacc tcgaaaattg tgagcgccca cagctctacg
     2881 ccgccaaaag ccagaacaag aaaggccagc agcaagaaca gag
//
LOCUS       DASREK010000069         8796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_370152, whole genome shotgun sequence.
ACCESSION   DASREK010000069 DASREK010000000
VERSION     DASREK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370152"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1727)
                     /locus_tag="VFA54_03730"
     CDS             complement(198..1727)
                     /locus_tag="VFA54_03730"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS49945.1"
                     /translation="MPSYLSSRRLGIAKGLPNFHKSAAFGCLLALLLAAPLGAQPRSS
                     AQDPFRDADRKLFAEIEKNNQLMANAGHLCDAIGPRLTGSEKLKAANQWTLAKFKEYG
                     LDAHLESLTIANGWRRGTASARLVEPAAETMTAASAGWSPSTNGPVRGPAIYVKAEKP
                     EDVEKFKGKLQGAIVLVTEPGKTPPTFAPAYALDSLAGEARENPDMPSQQRRRLLRQV
                     SEMFKREGVAAVVRDASKDFALFNMTSVGGSDYQIGEVPSAFVIHEDYLRLWRLLESR
                     QPVQVEVDIRNETIPGPVEVYNTVAEIRGATKPAEVVIVGAHLDSWDLGTGATDNATG
                     SSVVLEAARAIEASGLKPQRTLRFILFTGEEQGSVGARSYIRTHAGDLDKIQGVIIHD
                     TGTGKVKTFSLEKRYDIREAMDKIAAPLHEIGLEELTMRETNGSDHVPFREAGVPAFF
                     AMQDPADYRRTHHSQADTFDKIHKDDLIQGAKAVAAMAWNLSEYPEKLPRKKTDPPAG
                     R"
     gene            complement(1687..3516)
                     /locus_tag="VFA54_03735"
     CDS             complement(1687..3516)
                     /locus_tag="VFA54_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS49946.1"
                     /translation="MDAHRGEAPVKRTWRERLKALENVGPLLKMVWETSPLLGCISLV
                     SRLLRAVLPVATLYVGKLIIDAVVRSVRSGQVENGRIWRLVAAELALAVVSDLLARTV
                     ALADSLLGDRFTNRVSVELMRHASTLDLAHFEEPEFYDKLERARRQTMGRMQLMSQVM
                     GAAQDAVTLATLTSALAVFSPWLLAMLVCAVLPAFLGETHFASLSYSLLYRWTPQRRE
                     LDYLRWLGATQESAKEVKIFGLGGYLTERYKRLADLFYAENRDLVLRRAAIGSLLALV
                     GTAGYYGAYVVILYRTLHGALTVGDLTFLAASFSRSRTLIEGVLTNFSNITEQALYLD
                     DLFAFFRMQPRIRSKPGALPAPRPIRKGIEFRRVSFRYPDAERYALHNVSFELRPGQR
                     IALIGENGAGKTTITKLIARLYDPTEGEILLDGVDLREYDLEDWRREIGVIFQDYMRY
                     HMLLRENIAMGRVEHLENRPRIERAARLSLASDVAARLEKGLDQMVGRRFADGVDLSG
                     GEWQKVALARAYMRDAQLLILDEPTATLDARAEHRVFERFAELTRGKIAVLISHRFST
                     VRVADRILVLQDGEVLEEGTHEELVDRGGQYAELFELQASGYR"
     gene            complement(3673..4776)
                     /locus_tag="VFA54_03740"
     CDS             complement(3673..4776)
                     /locus_tag="VFA54_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS49947.1"
                     /translation="MADELSAEFEIRFPSGAAVTAALRLPMGTPSVTVLFGPSGSGKT
                     TVLRCLAGLDQPQAGKIACGSEVWYDAARGVWLPPQKRRIGFLFQDYALFPHLTIRQN
                     VEYGLGRWPAEQRSQRAQAMLELFDLGGLGGRKPRQLSGGQLQRAALARALAPGPRLL
                     LLDEPLSALDAPTRERLRGDLRRLLVRAGTPALVVTHDKTEAIALGDRMAVIIDGKVR
                     QTGTVQEVLSSPADAAVALSVGVETVAPARVLEEGNGLLKVQAGGAHITAVDPGRLKG
                     PHVFICIRAEDVILEKGHPSHGTARNHLAGRITAAIREGPVVRVVVDCGFPLVALVTK
                     QSYEDLGLREGETVAAVVKATSVHLIPRGEGLS"
     gene            complement(4761..5435)
                     /gene="modB"
                     /locus_tag="VFA54_03745"
     CDS             complement(4761..5435)
                     /gene="modB"
                     /locus_tag="VFA54_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HZS49948.1"
                     /translation="MDWQAVWLSLRLSCWTTLILLLAGTPIAYWLSFSPLRWKFLLEA
                     VVALPLVLPPTVLGFYILLAIGPNGPIGRFYTLVTGRLLPFSFQGLLAASVLYSLPFA
                     VQPFMSAFAAVDRKLVEASWCLGVSRRETFFRVIAPLSLPGLVTGLVLSFAHTLGEFG
                     VVLMVGGNLPGVTRTISISIYDQVQALDYAAAGTTSALLLGFSFVVLAVTYALQRRFW
                     AVWPMS"
     gene            complement(5632..6417)
                     /gene="modA"
                     /locus_tag="VFA54_03750"
     CDS             complement(5632..6417)
                     /gene="modA"
                     /locus_tag="VFA54_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZS49949.1"
                     /translation="MRTAWRTLPAFTALLLCGCRSQQPAKQLLVGAAADLQFALPQIL
                     DDFQRLHKGTAVKATYASSGSFFALILNQAPYDVFLSADATYPQELVKRGLADEDTLF
                     QYAAGRLVVWVPNSSPIDVAKLGVAALRDGSVHHIAIANPLHAPYGQAAEAAMKSLGV
                     YGAVQEKLVFGENISQTLQFVQSGAAEIGIVALSLASAPAVRNSGRYWEVPQSAYPRL
                     EQVGVIMKSAKDPDTARAFRAFLLSSKAQAILKQFGFDLPGEH"
     gene            complement(6423..6914)
                     /locus_tag="VFA54_03755"
     CDS             complement(6423..6914)
                     /locus_tag="VFA54_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZS49950.1"
                     /translation="MRLRGIAALLFAAMPAVVAAQAPAAATLTITGSVERPVTLSAAD
                     LAKMPRVSVEVRGRQATVTYTGVLLFDVLKLAGAPVGERLHGPFLASYVMAEARDGYR
                     VLFSLPEIDPGFTDAKVLVADHANGKELGSEQGPFRLVPADKSARRTVRMLQRLEVAT
                     APK"
     gene            complement(7254..7391)
                     /locus_tag="VFA54_03760"
     CDS             complement(7254..7391)
                     /locus_tag="VFA54_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49951.1"
                     /translation="MASACIAASPPAIAEGASGAEGIIGLPARAILLGTGIIALVNPW
                     K"
     gene            7419..>8796
                     /locus_tag="VFA54_03765"
     CDS             7419..>8796
                     /locus_tag="VFA54_03765"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS49952.1"
                     /translation="MRFVCALLLLASSLAAQDNKLTSSEAAQGWLLLFDGESLFGWTQ
                     AGKARWHVAAPALTADGGEGGWLRTNSAFADYIFKCDLRIGADTDAALFLRAEKKDLP
                     DESGYKLPIGVGIDAGRWHTYELQAVGDQISIVLDGRKISESKDLKNKAGYIYLSYNR
                     GSRVEFRNVKLRPLSMQPLLNGRNLFGWKPVNPPDPKAAKPQAPGLLKKFGHIIKPPK
                     SKEPASWTVVNGGIHVEKGPGRLETEAAYDDFVLQLDIRASSRDKKEHPNGGVFFRGD
                     PNVFESGYETQIENEYKAGNPSQPLEFGTGGIYNFQPARRVITRDNETFTETIVARGK
                     HFATWINGYPMADYEDNRPDAPAVPKGARTAAGTISLQVHDPSTELDVKNIRLATLPK
                     SASAPPAPVAAAAAPVANMPGPGGAPAPNVPAPAAAAPPVSAPAMPNLPAAGFSGAPA
                     PPAGGGADA"
BASE COUNT         1785 a         2809 c         2664 g         1538 t
ORIGIN      
        1 gctcaatagc gcggttcgcc cgctggcccg cgaccagcct cgagagcagc ggggtggatc
       61 tccggggcgc cgcgtttgaa gccggtcgga caacgatccc ccgcggcccg ccggcgaata
      121 caaatgagcc cgtgacttcc cgcccgaagt gcgcgagatc tccggcctgc ccaccgcgga
      181 agaacgtgga aacgagtcta gcgccccgcc ggcgggtccg ttttttttct cggaagcttc
      241 tccggatatt ccgaaagatt ccaggccata gccgcaacgg ccttggcgcc ctgaatgaga
      301 tcgtctttgt gaattttgtc gaaggtatcc gcttgcgagt gatgcgttcg gcggtaatcg
      361 gccgggtcct gcatggcgaa gaaggccggc acgccggctt cgcggaacgg cacatgatcg
      421 ctgccgttgg tctcccgcat cgtgagctct tcgaggccga tttcgtgcag cggcgcggct
      481 atcttgtcca tcgcctcgcg gatgtcatag cgcttctcca gagaaaacgt ttttactttc
      541 cctgtgccgg tgtcatggat gatcacgccc tggatcttgt ccagatctcc ggcgtgcgtg
      601 cggatgtagc tgcgcgctcc caccgatccc tgctcctcgc cggtaaacag gatgaagcgc
      661 agggtgcgct ggggtttcaa tcctgaggcc tcaatcgcgc gcgccgcttc cagcactacc
      721 gagctgcccg ttgcgttgtc cgtcgcgccg gttcccaaat cccagctatc cagatgcgcg
      781 cccacgatga cgacctccgc gggtttcgtt gcgccgcgaa tttccgcaac tgtgttgtaa
      841 acctcgaccg ggccggggat agtttcgttc cgaatgtcca cttccacctg gaccggctgc
      901 cggctttcga gcaggcgcca aagccggagg taatcctcgt ggatgacaaa ggcgctgggg
      961 acttcgccga tctgataatc cgatccgccg acgctggtca tgttaaacag cgcaaagtcc
     1021 ttggaggcgt cgcgcaccac cgcagcgaca ccctcccttt tgaacatctc gctgacctgc
     1081 cgcagcaggc gccgcctttg ctgcgacggc atgtcgggat tttcgcgggc ttcgccggcc
     1141 agcgaatcca gcgcataggc cggcgcgaat gtgggaggcg tcttgcccgg ctcggtcacg
     1201 agcacgatag cgccttggag ctttcctttg aatttttcga catcctccgg tttctccgcc
     1261 ttcacataaa ttgccggccc tcgcaccggc ccgttggtgg aaggcgacca gccggccgac
     1321 gccgcggtca tcgtctccgc cgccggctca accaggcgcg cgctggccgt tccccggcgc
     1381 cacccgttgg cgatggtgag gctttccaaa tgcgcgtcga gcccatattc cttgaattta
     1441 gcgagggtcc attgattggc ggccttaagc ttctccgatc ccgtcagccg cggcccgatc
     1501 gcatcacaga gatgtccggc gttggccatc aactggttgt tcttctcgat ctcggcgaag
     1561 agcttgcggt ccgcgtcgcg aaagggatcc tgggcggaag aacgcggctg cgcgccgagc
     1621 ggagcggcaa gcagcagcgc cagaagacag ccgaaggccg ccgacttatg gaaatttggc
     1681 agtcccttag cgatacccag acgcctggag ctcaaataac tcggcatact ggcctccccg
     1741 atcaaccagt tcttcgtgtg tgccctcttc gaggacttcg ccatcctgca acaccaggat
     1801 gcgatcggcc acgcggaccg tggaaaaacg gtgagaaatc aagaccgcta ttttacccct
     1861 ggtgagctcg gcaaagcgct cgaaaacgcg atgctcggcg cgggcgtcga gcgtcgccgt
     1921 cggttcgtcg aggatcagca attgggcgtc ccgcatgtag gcccgggcga gagccacctt
     1981 ttgccactcg cctccggaga ggtccacgcc atcggcgaag cgccggccca ccatctggtc
     2041 aagacccttt tccaaccgcg ccgccacatc ggaggccagg ctgagccgcg ccgcgcgttc
     2101 gatccgcggg cggttttcga gatgttccac gcggcccatg gctatgtttt cgcgcagcag
     2161 catgtgataa cgcatgtagt cctgaaaaat gacgccgatc tcccgccgcc agtcttcgag
     2221 atcatattcg cgcaggtcaa cgccgtccag cagaatctcg ccttccgtcg gatcgtataa
     2281 gcgagcaatg agtttcgtaa tggtcgtctt gccggccccg ttctcgccga tcagagcgat
     2341 gcgctgtccg ggacgcagct cgaaactcac gttgtgaagg gcgtatcgtt ccgcgtccgg
     2401 gtaccggaag ctgacgcgcc ggaactcgat gcccttccgg atcgggcgcg gcgccggcag
     2461 ggcgcccggt ttcgagcgga tgcgcggctg catgcggaaa aaagcaaaca gatcgtcgag
     2521 atagagcgcc tgctcggtga tattcgaaaa attcgttagt acgccttcga tcagcgtgcg
     2581 gctgcgggag aaactcgccg cgagaaacgt caggtcgccg actgtaagcg cgccgtgcag
     2641 cgtccggtaa agaatcacga cataagctcc gtagtagccg gcggtgccca ccagggccag
     2701 gagcgagccg atcgcggcgc gccgcaggac cagatcccga ttttcggcgt aaaacagatc
     2761 ggccaggcgc ttgtaccgtt ccgtcagata gccgccaagg ccgaaaattt tcacttcttt
     2821 ggcgctttct tgcgtcgcgc cgagccagcg caaataatcg agctcgcgcc gttgcggggt
     2881 ccagcggtaa agcagggaat aactgaggga agcaaagtgc gtctcaccca ggaaagcggg
     2941 aagcacggcg cagacgagca tggccagcag ccacggtgaa aagaccgcca gcgccgaagt
     3001 cagcgtggcg agagtgaccg catcctgggc ggcgcccatc acctgagaca tgagctgcat
     3061 gcgtcccatc gtctgccgcc gcgcccgctc cagtttgtcg taaaactccg gctcctcgaa
     3121 gtgggccagg tcgagcgtgg aggcgtgccg catcagttcc acgctcaccc ggttcgtaaa
     3181 ccggtcaccc aaaaggctgt cggcgagcgc cacggtccgg gcgagcagat cggaaaccac
     3241 ggctaaggcc agctccgccg ccacgagccg ccatatccgg ccgttttcga cctggccgct
     3301 gcgcacactg cgaaccacag cgtcgatgat gagcttaccg acgtacagcg tggccaccgg
     3361 caagacggcg cgcaggaggc gggagacgag agaaatacag cccagcagag ggctggtctc
     3421 ccagaccatc ttcaggaggg gcccgacgtt ttccagggct ttgagccgct cgcgccaggt
     3481 gcgctttacc ggcgcctcgc cccgatgagc atccatgagc tcaatgtagc atgggacttc
     3541 tgcgaggcat ccgagagccg tcccggagcg cgcagcttac gttgggctga aaagaaggcg
     3601 agcctgttgc tctggtcccg ttccttggcg gctgcgccca gccgccaacc ctccgccaga
     3661 gctggagcac agtcaggaga ggccttctcc gcgcgggatc aaatgcaccg aggtcgcttt
     3721 caccaccgct gccaccgttt cgccttccct gagtcctaga tcttcgtagg attgcttggt
     3781 gaccaaagcc actagaggaa agccgcaatc gacaactacc cgcaccacgg gaccttcgcg
     3841 aatggcggct gtaatccggc ctgcgagatg gttgcgagcc gtgccgtggc tcggatggcc
     3901 tttttcaaga atgacgtcct cggcccggat gcagatgaac acgtgggggc ccttcagcct
     3961 gcccgggtca accgcggtga tgtgtgcgcc gccggcctgg actttcagca gcccgttccc
     4021 ttcctccaaa acccgagccg gcgccaccgt ctcgactccg accgacagag ctacggccgc
     4081 atcggcgggc gaactcagaa cctcctgcac cgtgccagtc tgccgtacct tcccatcgat
     4141 gatgactgcc atgcggtcgc cgagagcgat cgcctctgtt ttatcgtggg taactacaag
     4201 agcgggggtc ccggcacgca ccagcaaccg ccggaggtcc cctcgcagcc gctcacgggt
     4261 gggcgcatcc agagctgaga gaggctcatc gaggagcagg agccgcggtc ccggcgcaag
     4321 ggcgcgcgcc agagccgccc gctggagctg gccgccggaa agctgacgcg gcttgcggcc
     4381 gcccaagcca cccagatcaa aaagctcgag cattgcctgc gctcgctggg agcgctgctc
     4441 tgccggccat cggcccagtc cgtattctac gttttgccgg atcgtgagat gcgggaacag
     4501 agcgtaatcc tgaaacagga agccgatgcg gcgtttctgg ggcggaagcc agacgccccg
     4561 cgccgcatcg taccaaactt cactgccgca ggcgattttt cccgcctggg gctgatcgag
     4621 gccggcgagg caccgcagca ccgtggtctt gccggaaccg gatggcccga acagcaccgt
     4681 tacggacggc gtacccatcg gcaggcgcag cgcggccgtt accgccgcgc cgctggggaa
     4741 gcggatttcg aattcagcgc tcagctcatc ggccatacgg cccaaaatct ccgttgcagc
     4801 gcataggtca ccgccagcac cacgaacgag aagccgagca acagcgccga agtcgttccg
     4861 gcggcggcat aatcaagcgc ctgcacctgg tcgtagatgg aaatggaaat tgtgcgcgtt
     4921 acgccgggca agtttccgcc caccatcagg acaaccccga actcgcccag agtgtgcgcg
     4981 aagctcagca ccagtccggt gacgaggccc gggagagaca gcggcgcaat cacacgaaaa
     5041 aatgtctctc gtctcgacac gccaagacac catgacgctt ccaccagctt tcgatccaca
     5101 gcagcgaaag ccgacatgaa aggctgtaca gcaaagggaa ggctgtaaag gacggacgcc
     5161 gccaaaagac cctggaaaga aaagggcagc agtcggccgg taacaagggt atagaaccgg
     5221 ccgatgggac cattaggccc aatggccaac agaatgtaga agcccagcac cgtgggcggc
     5281 agcaccagcg gcagagcgac cacggcctcg agcaggaact tccagcgcag cggggaaaag
     5341 ctgagccaat aagcgatcgg tgtccccgca agcagcagga tcaaagtggt ccagcacgaa
     5401 agccgcaagc tcagccagac tgcctgccag tccataactt aaccctccga acgatgctct
     5461 agcattcgcc gctgacgagc gccagccaca gaaaagccga gacgatcagc ttcccaaagg
     5521 cagcccccat ccaacgaccg ttcgtcctgc cggagtccgg cgaaaatcgg cagcaaaaaa
     5581 cttcacgaaa tttcagccgt gcaacgaacc ggcgcgctcg agccgagcgc ctcagtgttc
     5641 tcccggcaaa tcgaacccga attgcttcaa aatcgcctga gccttcgaac tcagcaggaa
     5701 ggcccgaaag gcccgcgccg tatccggatc ttttgcgctc ttcataatta ctccgacctg
     5761 ctcgagacgc gggtacgcgc tctgcggcac ttcccaataa cgtccgctgt tgcgcaccgc
     5821 cggcgcggat gccagagaga gggccacgat tccgatctcc gccgccccgc tctgcacaaa
     5881 ttgaagcgtt tgtgaaatgt tttctccaaa gacgagcttc tcttgcaccg caccatagac
     5941 gcccagagat ttcatcgcag cctcggcggc ctgcccatac ggagcgtgca gcggatttgc
     6001 aatagcaatg tggtgaaccg aaccgtctcg cagcgccgcg acgccgagct tcgcgacatc
     6061 gataggcgat gaattgggaa cccaaacgac caaacggccg gcggcgtact ggaagagggt
     6121 gtcttcatcg gccaagccgc gcttcacaag ctcttgcgga taggtggcgt cggcggaaag
     6181 aaaaacgtca tacggagcct ggttgagaat gagggcgaaa aaagatcctg acgacgcgta
     6241 ggtggccttc accgcagttc ctttgtgcag ccgctggaaa tcgtcgagga tctgcggcaa
     6301 tgcgaattgc aaatccgccg ctgcgcctac caggagctgc ttcgccggct gctgggaccg
     6361 gcagccgcac agcaaaagcg ccgtgaacgc cggcagggtg cgccatgcag tccgcataga
     6421 gactatttcg gcgcggtcgc cacttccagg cgctggagca tgcgcactgt gcggcgagcg
     6481 cttttgtcgg cggggacaag ccggaagggg ccttgctcgc tgcccagttc cttgccgttc
     6541 gcgtgatcgg ccaccagcac tttggcgtca gtaaatcccg ggtcaatttc aggcagcgag
     6601 aaaaggactc ggtacccgtc ccgggcctcg gccatgacat aactggcgag aaacggtccg
     6661 tgcagccttt cgccgaccgg cgcgccagcc aacttcagca cgtcaaaaag caagacgccg
     6721 gtataggtga cagtcgcttg ccgccctcgg acctccacac tcacccgcgg cattttcgcc
     6781 agatccgccg cgctgagcgt aaccgggcgc tccactgacc ccgttatggt cagagtggcg
     6841 gcggccggag cctgtgccgc taccacagca ggcatggctg caaaaagcag ggcggcgatt
     6901 cctctcaggc gcatgcgatc ctccctctcc cgagcgctga cttgtaaacg ttatattgtc
     6961 ttgtaaaagt gtcaaagttg caaggcagga tctcgagcgc gccgcagggc ctgctgacgg
     7021 cattgttcgc tctcttctcg aagctctccg gcttctcacg tccaagaagg gcttgcagcc
     7081 aggcgtatac cacgatgcag ggctttcgtt gatagcgcga ccttgggggc gcatgcccgg
     7141 aatccggcat cgaaatgccg gcgccgtctt tagcccttgg cgggaggatg gatgctcaaa
     7201 aaaaggagtg ctatactcca actgcgcaat aactcgagca ggaccccgaa tcactatttc
     7261 caggggttta cgagagctat gatgccggtt cccaaaagaa tcgctcttgc cggcagaccg
     7321 atgatgccct cggcgccgct ggcgccttct gcaatggccg gcggcgaggc cgcgatgcag
     7381 gcggatgcca tcgctctttg gggttaagat gaggccatat gagattcgtt tgcgctctgc
     7441 tcttgttagc cagttctctg gcagcccagg ataacaagct gacgtcctcc gaagcagcgc
     7501 aaggatggct cctgctgttt gacggggaat ctctgttcgg atggacgcag gcgggcaagg
     7561 cccgatggca cgtggcggcg cctgccctta ccgccgacgg aggcgagggc ggctggctgc
     7621 gcacgaacag cgcgtttgcg gactacattt tcaaatgcga cctccgcatc ggcgcggata
     7681 ccgacgccgc gctcttcttg cgcgccgaaa aaaaagacct gcccgacgaa tccggctaca
     7741 agctccccat cggcgtggga atcgacgcgg gccgatggca cacgtatgaa ctacaagccg
     7801 tgggagatca aatctccatt gtcctggacg ggagaaagat atccgagagc aaggatctga
     7861 agaataaagc gggctacatc tacttgagct acaaccgggg ctcgcgcgta gagttccgca
     7921 acgtgaagct gcggccgttg agcatgcagc cgctgttgaa cggcaggaac ctgttcggat
     7981 ggaaacccgt gaatccgccc gatcccaagg cagcgaagcc gcaggctccc ggccttctga
     8041 aaaaattcgg ccatatcatc aagccgccga agtccaagga acccgcgtcc tggacagtgg
     8101 tcaacggcgg cattcatgtg gagaaaggtc ccggccggct ggaaactgag gcagcctacg
     8161 atgatttcgt actgcaactg gacattcggg cgagctctcg ggacaagaaa gagcatccca
     8221 atggcggcgt gttcttccgc ggagatccca acgtatttga aagcggctac gaaacgcaaa
     8281 tcgagaacga atacaaggcg ggcaacccct cccagccgct cgaattcggc actgggggca
     8341 tctataattt ccagccggcg cgccgtgtaa tcacgcggga caatgagact ttcaccgaaa
     8401 cgattgtcgc tcgcggaaaa cattttgcaa cctggatcaa cggctatccg atggccgatt
     8461 acgaggataa ccggccggat gctcctgcgg tgcccaaagg ggcgcgcacg gcggcaggca
     8521 cgatcagcct gcaagtgcat gatcccagca cggaactgga tgttaaaaac atccggctcg
     8581 ccacgctgcc taaatcggcc tccgcgccgc cagcgcctgt ggcggcggct gcggcgcccg
     8641 tagcgaacat gcccgggccg ggaggagcgc cagcgccgaa cgtgcctgcg ccggcggccg
     8701 cagcaccccc ggtctccgct cccgccatgc caaatcttcc ggccgccggt ttctcgggag
     8761 ccccggcgcc gcctgcgggc ggtggagctg acgccc
//
LOCUS       DASREK010000070         8891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1028445, whole genome shotgun sequence.
ACCESSION   DASREK010000070 DASREK010000000
VERSION     DASREK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028445"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VFA54_03770"
     CDS             <1..1081
                     /locus_tag="VFA54_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS49953.1"
                     /translation="IFDADFIPPPDFLLRTVHYFTDPQVGMVQTRWTYHNRNQSFLTQ
                     VEAILLDGHFVLEHGARARSHRFFNFNGTAGVLRRAAIEDAGGWEHDTLTEDSDLSYR
                     AQLKGWKFLYVPQVECPSELPVEMVAFQVQQARWAKGLIQTGKKILPRLLRAPLPLRI
                     KTEGWFHLTCNISYPLMLVLSVLLLPAMIVRFYQGWFQMLYIDLPLFIASFCSISSFY
                     LVSQKELFPGRWKRTFLLIPFLMGTGIGLTLTNTQAVLEALLDIKSPFQRTAKYGNAK
                     SSKYRRRSHWLPWIDLILGSYFLLTVFYAISAENYFTVPFLLLFVVGYYYAAVLMLVQ
                     DNREALARWAQALLPWRLAYRQASR"
     gene            complement(1129..1962)
                     /gene="pgeF"
                     /locus_tag="VFA54_03775"
     CDS             complement(1129..1962)
                     /gene="pgeF"
                     /locus_tag="VFA54_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZS49954.1"
                     /translation="MGFYFDESGNLRLQEFERWPWLIHGFSTRSAGNLGASAGNPITQ
                     MRENQQRFLATLGGKGLDLVTVRQIHSAVVHMAETGAGRPGDALMLRQPGVAVGVKTA
                     DCFPVLVVDPRQRAAAAIHAGWRGAAQRIVEKTVGEMRRAFHSEPADLLAAIGPGIQA
                     CCFEVGPDVLEEFQSQFAEAHEFCYEEAPNPALDMLPRQTMTGKAHALMRRLDSQRGH
                     VDLAEAARRQLLAAGVLRGHIYDSGLCTMCDRERFYSHRREREQAGRMMAVIGIKRSK
                     S"
     gene            complement(2075..3370)
                     /locus_tag="VFA54_03780"
     CDS             complement(2075..3370)
                     /locus_tag="VFA54_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZS49955.1"
                     /translation="MKFAKSRFFRTAAAAFTAAALGQSLAPAETFMLDPARTQIRFAL
                     PAFLHTVHGTFKLSSGFVRFDPATGTAGGSIVVDSASGASGNEDRDRDMRIKVLESDR
                     FPQITFTPTRIVGRIPSTGRSEVQVQGVFSLHGAGHELAVNAAVTVAGDEMTADAHFV
                     VPYVDWGLKNPSTRLLRVGVKVTVDVHAAGRLASEPEGNKGEPARSSLFEKPEPEVLR
                     SGAPAVPHLKMRALGHLPHFFFAKAHAVPCIAALFVHWTALYLELEQSAGTQHAVYFA
                     DVILDDCLAWDVLKHDIGKREIELAGAEHRQIGPVILVHVGVCKIAQGLTSFCNHLSA
                     DVHSVHTAEHAGQRPQETPGAAANFEDAHFGRLAALADIQEVEQDVLLHVSFAGPEKL
                     IVRPIFLSRSHVVAGVFARASIPIPPHSLQQFRRPFHFQ"
     gene            complement(3504..4412)
                     /locus_tag="VFA54_03785"
     CDS             complement(3504..4412)
                     /locus_tag="VFA54_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49956.1"
                     /translation="MPAAGEKSAVLASLFMAVAAAALPARCAPPAGLADSGLDAGYQR
                     LYNLEFNRAHEVFRDWGRLHPQDPLAPASDAAAYLFSELDRLHVLQSELFVDDEKFEH
                     RKKLAPDEHVRAAFQTALEQAESLSRRILAHSPDDANALFADVLVFGLRADYAALIEK
                     RNFASLRYMKSGRMLAERLLSLDPSYYDAYLAIGVENYILSLRPAPVRWLLRAGGAET
                     DKREGIEKLKLTAEKGHYLQPYARLLLAVSALRDKDFARTQELLAGLARQFPRNPLYR
                     RELDRLRPKMAGTISSSANSIASQNR"
     gene            complement(4412..5770)
                     /locus_tag="VFA54_03790"
     CDS             complement(4412..5770)
                     /locus_tag="VFA54_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZS49957.1"
                     /translation="MPPTAVSTSTALYAQHVNPQWARLLDLLQMNVRYTRCSGAELFD
                     SEGRRILDFMSGYCVHNAGHNHPKIVLAIKTELDRCGPAMLQSHIPEIAGELAAALCA
                     RAGGQLRKVFFCSSGSEGVEAAIKFARAHAGRPGLIYADRAFHGLTCGALSLMGDPSW
                     REAFGPLLADAQSVPFGDIGALERALSGKRAAAFIVEPVQAEGGVNVPGPDYLPAAQA
                     LCRRYGALLVLDEIQTGMYRTGRFLAAHHFGLEPDIVILAKALSGGLVPCGAVLMSDR
                     VYQSVYSSLRRAVIHTSTFSENSLAMRAALATLEVLEEERLGERAQAAGARLRGGLEK
                     ALRGYEMIAGVRGMGLLCGIVFQPPRHMRLKIPFEAFRKIHPGMFGQMLVMRLFREKA
                     MLTQVCGNNFMVLKAAPPLAIREEQIDEFIAAVREVVEFVHSSLTFWSDALNLARRVV
                     NI"
     gene            6190..>8891
                     /locus_tag="VFA54_03795"
     CDS             6190..>8891
                     /locus_tag="VFA54_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS49958.1"
                     /translation="MNISETFIRRPIATTLLMFAVALFGVLAYRALPVADLPSVDFPT
                     LVVSANLPGADPDTMASAVATPLERQFTTIAGLDSMISVNSLGSTQITLQFDLARSQD
                     SAAVDVETAISEAMPLLPPNMPTPPSFRKYNPADIPIMFFGLTSPTLPIWVLDDYAEN
                     KIAQRLSMVNGVAQVNVQGAQKFAVRVQVDPSKLAARNIGINEIATALKSWNVNLPTG
                     TLYGPHQAFNVLADGQLTNAAAYRPMVVAYRNGAPVRLEQVANVIDNVEDDKQGSWLY
                     NREGVRKAINLIIFRQPGANTIEIVDEIKKLIPEFQAQLPPSVQLSIRGDRSENIRSS
                     FHDMQFTMLVTLGLVIMVIFLFLRNSSATLIPGMALPFSIVGTFAIMYLLDYSLDNLS
                     MMALILSVGFVVDDAIVMLENVVRHMEHGEGPLQAAFSGSKEIGSTIVTMTVSLAAVF
                     IPVLFMGGILGRLFREFAVTICVAILISGMVSVSLTPMLCSRILRPPAEARHGRLYAA
                     TERFFQGMLRVYAGSLRWVLRRRPVMMAVFFGVLGGTTYLFVKIPKGFIPTPDMDQFY
                     VATEAAQGTSYYQMVELQKKIADIVAADPNVDSALSTVGGQQNIGSNTGRMSVILKPR
                     NERELNVMQIIERLRPKVSGFPNVRAYLTAPPAIRIGGHITKSEFQFTVQGPNTTELY
                     AQAARLEREVARLPGLQDVNTDLQIKNPQVRIAIDRDKAAALHLNVADIEAELYNAYG
                     PNWASTIYLPTSQNRVLLELLPSYQARADLLSSLYFRSSDGHLVPLGSFAKLTEDAGP
                     LTVNHSGQLPSVTISFNLKPGVALGDAVSEIQDIALRNLSPTITTSFQGAAKAFQDSL
                     RNLGILLTVAVMIVYIVLGVLYESYVHPITILSGLPSAGFGALIT"
BASE COUNT         1712 a         2740 c         2679 g         1760 t
ORIGIN      
        1 gattttcgat gcggacttca tcccgccccc tgatttcctg ctgcgcacgg tccactactt
       61 caccgatccc caggttggca tggtgcagac gcgctggact taccataacc ggaatcagtc
      121 cttcctgact caggtggagg cgattctgct ggacggccac ttcgtgctgg agcacggcgc
      181 gcgggcccgg tcgcatcggt tcttcaattt caacggaact gccggggtgc tgcggcgcgc
      241 agccattgaa gacgccggcg gctgggaaca tgatacgctc actgaagact cggacctgag
      301 ctaccgcgct caactgaagg gctggaagtt tctgtatgtt ccgcaagtcg aatgtccttc
      361 ggagctgccc gtcgagatgg ttgcgttcca ggtgcaacaa gcgcggtggg cgaagggact
      421 gattcaaacc ggcaagaaaa tcctgccgcg tcttctgcgg gcgcccctgc cgctgcggat
      481 caaaacggag ggctggttcc acctgacctg caacatttcc tatccgctga tgctggtgtt
      541 gtcggtgctg cttctgccgg cgatgatcgt gcgtttctac cagggctggt ttcagatgtt
      601 gtatatcgac ctgccgctgt ttatcgcctc tttctgctcg atctcttcgt tttatctagt
      661 cagccagaag gaactgtttc ccggccgctg gaagcgcacc tttctgctga ttccttttct
      721 catgggaacc ggcatcggcc tgacgctcac caatacccag gcagtgctcg aagcgttgct
      781 ggacatcaag tcgcctttcc agcgcaccgc caaatatgga aacgccaaaa gctcgaaata
      841 tcgccgccgg agccactggc tgccgtggat tgacttgata ctcggctcgt attttctctt
      901 gaccgtgttc tacgccatct cggcggagaa ttacttcacc gtgccgttcc tgctgctttt
      961 cgtcgtcggt tattattacg cggcggtttt gatgctcgtt caggataatc gggaagcgct
     1021 ggcgcgttgg gctcaggcgc tgcttccctg gaggcttgcc taccgccagg catcgcgata
     1081 agccgcgccg ctgtggtgcg ccttgggtgc ggcagatttc agcgccggtt agcttttgga
     1141 ccgcttgatt ccaatcaccg ccatcatgcg gcccgcctgc tcccgctcac ggcggtggga
     1201 gtagaacctc tcccggtcac acatagtgca caggccggaa tcataaatat gacccctgag
     1261 aacaccggcc gccagtaact ggcgccgggc ggcttcggcc agatccacat gcccgcgctg
     1321 ggaatcgagc cgccgcatca gagcatgcgc tttgccggtc attgtctgcc gggggagcat
     1381 gtcgagagcc gggttcggag cctcttcgta gcagaactca tgggcttcgg cgaactgcga
     1441 ttgaaactct tcgaggacgt cagggccgac ctcaaaacag caagcctgga tccccgggcc
     1501 gatcgcggcc aacaggtccg cgggctccga atggaaggcc cgcctcatct cgcccaccgt
     1561 tttttccacg atgcgctggg cagcgccgcg ccagccggca tgaatggccg ccgccgcacg
     1621 ctgacgggga tcaacaacaa gcaccggaaa gcagtcagcg gtcttgacgc ccaccgcgac
     1681 ccctggctgg cgcagcatca gagcgtcgcc cggccggccc gcccccgttt cagccatatg
     1741 gacgacggcc gaatgaattt gccgcacagt cacgagatcc aggcctttgc cgcccagcgt
     1801 tgcgagaaac cgctgctgat tttcccgcat ttgggtgatg gggttgccgg ccgacgcgcc
     1861 aagatttccg gcagaccgtg tgctgaaacc gtgaatcagc cagggccagc gctcgaactc
     1921 ttgcagccgc aagttgccgg attcgtcgaa ataaaaaccc atacgctaca ctgaatctta
     1981 gcaatcggag cggcgggccg agggacccgc ggagtcgcgg ccggatcggt ctcttcgcac
     2041 ccgttgctct cgaaccgcag tcgggcgggc ggaattattg gaaatggaat ggacgccgga
     2101 attgctggag gctatgcggc gggattggga tactcgcgcg cgcgaaaacg cccgctacta
     2161 cgtggctccg cgaaagaaaa attggacgga cgatgagttt ttccggtccg gcgaaagaga
     2221 cgtgcaggag cacgtcctgc tcgacctcct gaatatctgc cagggccgca agccggccga
     2281 aatgcgcgtc ctcgaaattg gctgcggcgc cgggcgtctc ttgcgggcgc tggccggcgt
     2341 gttcggccgt gtggacggag tggacgtcag cggagagatg attgcaaaag ctcgtcaagc
     2401 cctgcgcgat tttgcaaacg cctacgtgca ccagaataac ggggccgatc tggcgatgtt
     2461 cggcgccggc cagttcgatt tcgcgttttc ctatatcgtg tttcagcaca tcccaagcaa
     2521 ggcagtcgtc gagaattacg tccgcgaagt acaccgcgtg ctgcgtcccg gcggactgtt
     2581 caagttccag gtacagggct gtccagtgga cgaacagagc tgcgatacat ggaacggcgt
     2641 gggctttcgc gaagaagaaa tgcggcagat ggccgagcgc acgcattttg agatgcggta
     2701 ctgctggggc gccggaacgc aatacttctg gctctggttt ttcaaacagt gagcttcgcg
     2761 ccggctcccc cttatttccc tccggctcgc tcgccagacg ccccgccgcg tgaacgtcaa
     2821 ccgtcacctt gacgccgacg cgcagcaggc gcgtgctcgg atttttcagg ccccaatcca
     2881 cgtaaggcac cacgaagtga gcgtcggcgg tcatttcatc gccggccacg gtgacagcag
     2941 cgttgacagc caattcatgc ccggcgccat gcaggctgaa tactccttgc acttgcacct
     3001 ctgaccggcc tgtcgaagga atccggccga caatccttgt gggcgtaaag gtgatctggg
     3061 gaaaccggtc gctctcgaga acctttatgc gcatatcgcg gtcgcgatcc tcgttgccgc
     3121 tggcgccgct ggcgctgtct acgacgatgc tcccgccggc ggtgccggtc gctgggtcga
     3181 agcgaacaaa tccgctgctg agcttgaacg ttccgtgtac tgtatgaagg aatgcgggca
     3241 gtgcaaagcg gatctgcgtg cgcgccgggt cgagcataaa cgtctcggcc ggcgcgagcg
     3301 actgtcccag cgcagccgcc gtgaaggctg cggcggcggt tcgaaagaac cggcttttcg
     3361 cgaatttcat ggccgtttcc taaaaacagc tccgcgttgg cgcgcggctt gctcccgtaa
     3421 gcagagcttg cagacgccgc cttcaggata ccgccgggca gcgatggtaa gtgttaatcc
     3481 tcagaaggcg cccccaccca gtgtcaacgg ttttgagaag cgatgctgtt ggcggacgaa
     3541 gaaattgtac cggccatttt cggacgcagc ctatccagtt cccgccggta gagaggattg
     3601 cggggaaact gcctggcaag ccctgcgagc agctcctgcg tccgcgcaaa atccttgtca
     3661 cgcagcgcgg agacggccag cagcaaacgg gcgtagggtt ggaggtaatg tcccttttcc
     3721 gccgtaagct ttaatttttc gatcccttct ctcttgtccg tctccgcgcc gccggcgcgc
     3781 agcagccacc ggaccggcgc gggccgcagg ctgagaatgt agttctcgac tccgatagcg
     3841 agatatgcgt cgtaatacga cggatcgagg ctgagcagcc tttcggcgag catccgcccg
     3901 cttttcatat agcgtaaaga ggcgaaattt cgtttctcta tgagggcagc atagtcggcg
     3961 cgaagcccaa aaaccagcac gtcggcgaac aaggcgttgg cgtcgtccgg agaatgcgcc
     4021 agaatgcggc gcgagagcga ctccgcttgc tccagcgctg tttggaaagc cgcccggacg
     4081 tgttcatcag gcgccagctt tttgcggtgc tcgaattttt catcatccac aaacaactcg
     4141 gactggagga cgtgcagccg gtccagttcc gaaaagagat aagcggcagc gtctgaggcg
     4201 ggagcgagcg ggtcttgcgg gtgcagccga ccccaatcgc ggaatacctc gtgcgcgcgg
     4261 ttaaactcca ggttgtacag gcgctggtaa ccggcgtcca gaccggaatc ggcgaggccc
     4321 gcaggaggag cacagcgggc gggcaaagcc gcagcggcaa ccgccatgaa caggcttgcg
     4381 agcaccgccg atttttcccc agccgccggc atcaaatatt gaccactctc cgcgcaaggt
     4441 tgagcgcatc cgaccaaaac gtcaacgagg agtggacaaa ctcgaccacc tcgcggaccg
     4501 ctgcgatgaa ctcgtcgatc tgctcttccc gaatcgccaa tggcggggcc gctttcagca
     4561 ccatgaaatt gttgccgcaa acctgcgtaa gcattgcttt ctcgcggaac aggcgcatga
     4621 cgagcatctg gccgaacatg cccggatgga ttttccgaaa cgcctcgaac ggaatcttca
     4681 gccgcatgtg gcgcggaggt tgaaacacga ttccgcagag aaggcccatg ccgcgcacgc
     4741 cggcgatcat ttcgtagccg cgcaaagcct tctccaggcc gccgcgcagc cgcgcgcccg
     4801 ccgcttgcgc ccgttcaccc agccgctctt cttcgagtac ttccaaggtc gccagggcgg
     4861 cgcgcatggc caggctgttc tcgctgaacg tggaagtatg gattaccgcg cgccgcaaag
     4921 agctatagac cgactgatag acgcggtcgg acatcaggac agcgccgcag ggaaccagac
     4981 cgccgctgag agctttcgcc aaaatcacga tgtccggttc gaggccaaag tgatgcgccg
     5041 caagaaagcg gccggtgcgg tacattccgg tctgaatttc atccagcacc aaaagcgcgc
     5101 cgtagcggcg gcacagggct tgtgcggccg gcaaatagtc cggcccggga acattgactc
     5161 cgccttcggc ttggacaggt tcgacgatga atgcggcggc gcgctttccc gacagagctc
     5221 tttcgagcgc gccaatatcg ccaaaaggca cgctctgcgc gtcggcgagc aagggtccaa
     5281 aagcttcgcg ccaggatgga tcgcccatga gcgacaaggc cccgcacgta aggccgtgaa
     5341 atgcccggtc tgcgtagatc agaccgggcc ggccggcgtg cgcgcgggcg aatttgatgg
     5401 cggcctcgac tccttcgctg cccgaactgc aaaaaaatac ctttcgcaat tggccgccgg
     5461 cgcgcgcgca gagcgccgcg gcgagttctc cggcgatctc cggtatatgg ctttgcagca
     5521 tggccggccc gcagcggtcc agttcggtct tgatggcgag gacgattttc gggtggttgt
     5581 ggccggcgtt gtgtacacag tagccggaca tgaagtcgag aatgcgccgc ccttccgagt
     5641 caaagagctc ggcgcccgag cagcgcgtgt aacgtacatt catctgcaaa aggtcgagaa
     5701 gcctggccca ttgcggattc acgtgttgtg cgtacagcgc tgtcgacgtg gaaaccgcgg
     5761 ttggtggcaa agggagttta acctcgcaaa ggaagtttac agcccaaaag gccgtggggc
     5821 aaacggaaac cttgccctgc cgataattgc gatcatccgc cttctacaga cgcgggccgc
     5881 gaaactcggg tttccttgcg ccggcgggga gagcaaaaac gagcgcggct gggcttcgaa
     5941 aagagccttc cattcggccg tcaagtaacg ggaaacggct gcatgccgcc ttcctgcttt
     6001 gataatctta agtggcgccg cgcgacggaa agcgagcagc cctcgcgcgc aacagcggcg
     6061 caggtccctc tggacggccc cggcgccagc ggcgtcttag gcgggaagca ccggcctggg
     6121 gtcgaagagc cggcgccgcc ggccgcaaga ccgcaaattt acggaaagcg accaggatgg
     6181 catgaagccg tgaacatctc cgagaccttc atccggcgcc cgattgcaac cactctgctg
     6241 atgttcgccg tggctttgtt cggcgtcctg gcctatcgag cgctgccggt agccgacctg
     6301 ccgagcgtag attttccgac cctggtcgtc tccgccaacc ttccgggcgc cgatccggac
     6361 actatggcgt cggcggtcgc aaccccgctc gaacggcaat tcaccaccat cgccggcctg
     6421 gattcgatga tctctgtaaa ttccctcgga agcacgcaga tcaccctcca gttcgacctc
     6481 gctcggagcc aggacagcgc cgcggtggac gtggaaacgg cgatctcgga agccatgccg
     6541 ctgctgccgc cgaacatgcc cacgcctcct tcgttccgca agtacaatcc ggcggatatt
     6601 ccgatcatgt ttttcggcct gacttcgccc acccttccga tctgggtgct cgacgattac
     6661 gccgaaaaca agatcgcgca gcggctttcg atggtcaacg gcgtggctca ggtaaacgtg
     6721 cagggagcgc agaaattcgc ggtgcgcgtt caggtggacc cgagcaagct ggcggcgcgc
     6781 aacatcggaa tcaatgagat cgcaaccgca ctgaagagct ggaatgtgaa tctgccgacg
     6841 ggcacgctct acggcccgca ccaggccttc aacgtgctgg ccgacgggca actcaccaat
     6901 gcggcggcgt accggccgat ggtcgttgcg taccgcaacg gcgcgcccgt ccggctggag
     6961 caggtcgcca atgttatcga caacgttgag gacgataagc aagggtcgtg gctttacaac
     7021 cgggagggcg tgaggaaggc gatcaatctc atcattttcc gccagccggg cgccaacacg
     7081 atcgagattg tcgatgagat caagaaactg attccggagt tccaggcgca gcttccgccg
     7141 tctgtgcagc tcagcattcg cggcgaccgg tccgaaaata ttcgcagctc cttccatgac
     7201 atgcagttca ccatgctggt gacgctcggt ctcgtcatca tggtgatttt tctcttcctg
     7261 cggaattcct cggccacgtt gattccgggg atggcgctgc ctttctccat cgtcgggacg
     7321 ttcgcgatca tgtacctgct cgattacagc ctggacaacc tctcgatgat ggcccttatc
     7381 ctgtcggtgg gatttgtggt ggatgacgcc atcgtcatgc tggaaaacgt ggtgcgccat
     7441 atggagcatg gcgaggggcc gctccaggcg gccttcagcg gatcaaaaga aatcggatcg
     7501 accatcgtca ccatgaccgt gtcgctggcg gcggttttca ttccggtgct gttcatgggc
     7561 ggaatcctgg ggcgtctctt tcgcgaattc gccgtgacca tctgtgtcgc tatcctgatc
     7621 tctggcatgg tctcggtcag tctcacgccc atgttgtgca gccgcattct acggcctccg
     7681 gcggaggccc gccacggccg cctctacgcg gccaccgagc ggtttttcca gggaatgttg
     7741 cgcgtctacg cgggcagcct gcgatgggtg ctgcggcgcc gccccgtcat gatggcggtg
     7801 ttctttggcg tgctgggcgg cacaacgtac ctgttcgtaa agattcccaa gggattcatt
     7861 cccactccgg acatggacca gttctatgtc gccaccgagg ccgcccaggg cacttcctat
     7921 tatcagatgg tcgaactgca aaagaagatc gccgacatcg tggctgccga tcccaatgtc
     7981 gattccgctc tctccaccgt aggcgggcag cagaatatcg gatcgaatac cgggcgcatg
     8041 tcggtgatcc tgaagcctcg caacgaacgc gagctcaatg tgatgcagat catcgagcgg
     8101 ctgcggccaa aggtctccgg ctttccgaac gtgcgggctt acctcactgc gccgccggcc
     8161 atccgcatcg gcggccacat caccaagagc gaattccagt ttacggtgca ggggccgaat
     8221 actaccgagc tttacgcgca ggcggcgcgc ctggagcgcg aggtggctcg tttgccgggg
     8281 cttcaggatg taaataccga cctgcaaatc aaaaacccgc aggtgcggat cgcgattgac
     8341 cgcgacaaag cggctgcttt gcatttaaac gtggccgaca tagaagccga actgtacaac
     8401 gcctacggtc cgaactgggc ttcgaccatc taccttccca caagccagaa tcgcgttctg
     8461 ctcgagctgc tgccatccta tcaggcccgg gcggatcttt tgtcgtccct gtatttccgg
     8521 tcgagcgacg gccatttggt ccccctgggt tccttcgcca agctcaccga ggatgccgga
     8581 ccgctgactg tcaaccactc gggccaactg ccttccgtga cgatctcgtt caacctcaag
     8641 cccggagtag cgctcggaga tgcggtctcg gagatccagg acatcgccct gcgcaacctg
     8701 tcccccacga tcacgacgag ttttcagggc gcggcgaaag cctttcagga ctcgctgcgg
     8761 aatctcggca ttctgttgac ggtcgcggtc atgatcgtct atatcgttct cggcgttctt
     8821 tacgaaagct acgtccatcc gatcacgatt ctttccggct tgccctcggc cggttttggc
     8881 gccctcatca c
//
LOCUS       DASREK010000071         9704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1028982, whole genome shotgun sequence.
ACCESSION   DASREK010000071 DASREK010000000
VERSION     DASREK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9704
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028982"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /gene="rimO"
                     /locus_tag="VFA54_03800"
     CDS             <1..1360
                     /gene="rimO"
                     /locus_tag="VFA54_03800"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZS49959.1"
                     /translation="DSEVMMGLLVQRGHELTCHAAEADVLVVNTCSFIQPAQQESVNA
                     ILEMAGHKKSGQARKLIVAGCLVERYRDEIRREIPEVDAVIGANEIERIIDVCENAGS
                     PRAAPASERAPYLYHDLTPRVRATSRHLAYIKIAEGCDHPCSFCVIPQFRGRFRSRRW
                     ESVVAEARNLFDQGVREITLIGQDTTCFGEDYGLKDGLAFLLDRLARLENAGWIRVLY
                     VYPNKVTQKLLDTMAAHETLCKYIDVPFQHASASVLKRMKRGGSGDVFLRLIERIRKT
                     MPGVALRTSMIVGFPGESEADFRQLCDFLAAAQFDHLGVFAYSDEETSASYYLDGKID
                     ARTIYNRKRRLMAVQRRISRAKKRSLVGQTLPILIDGPSPETDLLWEGRLAGQAAEID
                     GKTYLNDFEGGCPEPGTFGWARITESRDYDLIGTLLASSREYTPAARRSTPGEEPFRI
                     LQ"
     gene            1357..1542
                     /gene="yacG"
                     /locus_tag="VFA54_03805"
     CDS             1357..1542
                     /gene="yacG"
                     /locus_tag="VFA54_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZS49960.1"
                     /translation="MKCPICKKDVSERDRYYPFCSERCKLIDLGNWASGQYVISTPIR
                     PDSEQKPPEGESDGDGE"
     gene            1532..2008
                     /locus_tag="VFA54_03810"
     CDS             1532..2008
                     /locus_tag="VFA54_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZS49961.1"
                     /translation="MASSGRPPAAIWLATWGGCGYFPKGPGTAGSLGGAAVAWVLVRW
                     AGWPPAALAAAALGLLGPGVWASSRAARYWGQEDPQRVVIDEVLGQWLALSVAPAAEW
                     RYWLAAFALFRIFDIWKPFPVRSAEQLAAGWGIVADDLVAGVYAAVVLLALRRLHF"
     gene            2031..3062
                     /locus_tag="VFA54_03815"
     CDS             2031..3062
                     /locus_tag="VFA54_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZS49962.1"
                     /translation="MGLVRKEGGQRPQISSVKPASAIAGGEFVVQGARFRANGEERPR
                     VRFGEVEAPLVVGADDFMVVRVPEGATVGELIIENRDASSSPYACNLGFPIAENLHPV
                     ANPVVDMHGNIYTTFSGSRGQKVPVSVYKIDPNYEMKPFVKDLMNATGLALDREGMLL
                     VSSRFDGTIYQVAPSGKMSIYSEGMGIATGLALDEEGNLYVGDRSGTIFKIAPSLAPA
                     RQIFVFATLEPSISAYHLAVAPDQSLFVTGPTTSSFDSIYRISHNGEVEVYYRGLGRP
                     QGMAFDAEGRLYVAASLGGRKGIWRFGPERKPELVLSGPGIVGLAFASAHTAIVATNN
                     ALYRVDLAP"
     gene            3174..4424
                     /locus_tag="VFA54_03820"
     CDS             3174..4424
                     /locus_tag="VFA54_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZS49963.1"
                     /translation="MTAEILAVGSELLTPQRVDTNSLYLTQRLNDLGIEVVSKAVAGD
                     DRVRLALLLGQMRERTELVIVTGGLGPTEDDVTRDAAAQVCGRELVFRQEVLDQIEAR
                     FRSLRRTMAPINRRQAYIIDGAEILPNQHGTAPGQWYKDAAGILILLPGPPNELKPMF
                     ESECVPRLRLAAPEQYIATFMMRVAGMPESDLDQLISPIYTRYTNPVTTILAAPGDIQ
                     IHLRARGASESEARVVVEELARQIEAVLGNRVYSRSAATLEEVVGRILTERNATLAVA
                     ESCTGGLIAERITSVPGSSRYFAGGWIPYSNRAKREWLGVSQETLETQGAVSAQVARE
                     MAQGARARACSSLGLSVTGVAGPTADAGGGESGPKPVGLVYIGLADEAGCEVKERQFL
                     GDRDRIRAQASQMALDMVRRRFLA"
     gene            4563..5129
                     /locus_tag="VFA54_03825"
     CDS             4563..5129
                     /locus_tag="VFA54_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZS49964.1"
                     /translation="MKALGIIAAVVVLAGVALSSSFVSRRNEMVAQREAIKNQWAQVD
                     TALQRRADLIPNLVETVKGYASQERAVFESIAQARAAMAGAKTPAERIAANDQLSGAL
                     SRLLVVVENYPQLKSNENFLRLQDELAGTENRIAVERRKYNEVVQAYNTNIQLFPNNI
                     VAAISGFQREEAYFRASDSARQAPAVKF"
     gene            complement(5194..5721)
                     /locus_tag="VFA54_03830"
     CDS             complement(5194..5721)
                     /locus_tag="VFA54_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49965.1"
                     /translation="MRTKLLALSALASALLLADAAGGVKWTMPAGWKSQGARPMRAAT
                     YEIPAAPGDKEAGECGVFYFGPGQGGTVEDNVNRWVDQFEQPGGKPSRQSAQIHTQTI
                     NGLRVTTVDASGTYTGAGGPMAQQVIRKPGYRLLGAIVEVPRGPVFFKFTGPAKTVAA
                     NQGAFQAMLKSLSRS"
     gene            5807..6889
                     /locus_tag="VFA54_03835"
     CDS             5807..6889
                     /locus_tag="VFA54_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49966.1"
                     /translation="MLERYRAALSWLAHSGIQAESGGLARYYRAEAGEYKDISTEITA
                     YGIQAYLQLPLPGESGLLSNALRAGQFLCYDATDPQTGLLFFELSPNGSPASRLAYFF
                     DCAIIIRSLIALWNTTADPMYLERAERCGAGMMGRMSRVDGSFFPMLDLETGIPSSAP
                     GGWSLEAGPYQLKAGLSFIELAEVTSSGLFSRTADALLDWCLRQHELFLRGEDRPDRM
                     ADRLHAYCYFLEGLLPFVEKRFECTLVLQAGINRVETLAAEASAALERCDVLAQLLRL
                     RLLCDYMGLLELDASAASREAEALLAFQLHTDDRRTNGAFAFGRRDGQLSCHANPAAT
                     IFALQALRMWKEHQSGELRMSWHDLI"
     gene            6912..8309
                     /locus_tag="VFA54_03840"
     CDS             6912..8309
                     /locus_tag="VFA54_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZS49967.1"
                     /translation="MPDFDLIPRPVPAVETKYRRIVTPIPVPESIPVLERLFACEPVA
                     MRGQPPIVWDHAEGITVHDAWGNRWLDWSSGVLITNAGHGRPEIAAAIHDQVNHQLLT
                     NYCFPSEIRARLVEKLAGLLPEPLKKIFLLTTGSETVECAVKLCRTHGIKAGGRRKIT
                     IVSFHNAFHGRTLGSQLAGGIPALKDWIVNLDPDFVQVPFPDGYRCRDTSFQRFERSL
                     DEARVKPERVAGVVLETYQGGDAAFAPPAYMQELRRWCDRHAALLVCDEVQAGFGRTG
                     TLFGFEHYGIVPDLACFGKGITSSLPLSAVAGRPDVMDLHPKGSMTSTHTGNPVCCAA
                     ALANIDVILKEDLAGNARRVGETLHESLRALKADFPQIAAVMGKGLVAGLPVIDPLTK
                     EPDSHLAYDAVRRCYEKGLLMFNPVGFGGATIKIAPPLTTNAEAAVEGVSVLREALAE
                     AIAARQSTLQEVVGG"
     gene            8306..9532
                     /locus_tag="VFA54_03845"
     CDS             8306..9532
                     /locus_tag="VFA54_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS49968.1"
                     /translation="MSAPAVVLIGAGMIAHDQILPSLYHMQRQGLVGEISVCASHSGA
                     LKRLAGASIFRRAFPGQSFRPYPDFSADPDEPHPNLFREVIEKMQAGGIAVVAVPDPL
                     HFDVIMAALRAGLHVCSVKPLVLTARESVVIEREAFARGLVVGVEYHKRFDDRSLLAR
                     RKYREGAFGEFRLGSACLLEKWYYRHSNFQNWMTTEHSDAFTYIGCHYVDLVHFITGL
                     LPEAVSVYGIRDRFPNGKEGFLWTDARVIWSNGACLNVQNALGFPDAAPGTNTQGLTL
                     YCASGDTGTLISHSDQYRGLKYSYASKPDEPGAAQYAEPSPDYFQYVDLGGPGLTPVG
                     YGYRSIEYILRACLRAGAETSLAKRQELLKEWDEAGIMATPANSRYNEQVIEAARESI
                     RNRGREAPIKRDPPAA"
BASE COUNT         1753 a         2929 c         3122 g         1900 t
ORIGIN      
        1 agacagcgaa gttatgatgg gcttgctcgt ccagcgcggc catgaattga cctgtcatgc
       61 cgccgaagcg gatgtgctgg tggtgaatac ttgcagcttc atccagcccg ctcagcagga
      121 atctgtaaat gccattctgg agatggccgg gcacaagaag tccgggcaag cgcgcaagct
      181 catcgtcgcg ggttgtctgg tcgaacggta tcgggacgag attcgccgcg agatcccgga
      241 agtggacgct gtgatcggcg ccaacgaaat cgagcggatc atcgacgttt gcgagaacgc
      301 cggcagccct cgggccgctc ccgcatcgga gcgggcgcct tatctgtatc acgatttgac
      361 gccgcgggtg cgggccacat cccggcactt ggcctatatc aaaatcgccg agggctgcga
      421 tcacccctgc tctttttgcg tcattccgca gtttcgcggc aggtttcgca gccggcgctg
      481 ggaaagcgtg gtggcggagg cgcgcaacct cttcgatcaa ggcgtgcggg agatcactct
      541 cattggccag gatacgacct gtttcggcga ggactacggc ttgaaggacg ggctcgcttt
      601 tctgctggat cgcctggcga ggctggaaaa tgcgggctgg attcgcgtgc tttacgtcta
      661 ccccaacaag gtcacccaga agctgctcga caccatggcc gcccacgaaa cgctttgcaa
      721 atacattgat gtgcccttcc agcacgccag cgcatcggtg ctgaaacgca tgaagcgagg
      781 cggctcgggc gacgtctttc tccggctgat tgagcgcatt cggaagacaa tgccgggcgt
      841 cgccctgcga acgagcatga tcgtgggctt tcccggagaa agtgaggcgg attttcggca
      901 gttgtgcgat ttcttggcgg cggcgcagtt cgaccatctc ggtgtttttg cgtattccga
      961 cgaagaaacc agcgccagct attacctgga cggcaaaatc gatgcgcgca caatttacaa
     1021 tcgcaagcgg cgcctgatgg cggtgcagcg caggatttcg cgcgccaaaa agcggtcgct
     1081 ggtgggacaa actctgccca ttctgattga cgggccttcg ccggaaacag atctgctgtg
     1141 ggaaggccgt cttgccggcc aggcggcgga gatcgacggg aaaacctatt tgaatgattt
     1201 cgaaggcggc tgccccgagc cggggacctt cggatgggcg cgcatcaccg aatcgcgcga
     1261 ttacgacctg attggcacgc ttttggcttc atcccgggag tacacgccgg cagcccggcg
     1321 gtcaacgcca ggggaggagc cttttcggat tttgcagtga agtgtcctat ctgcaagaag
     1381 gatgtatcgg agcgggaccg gtattacccg ttttgcagcg agcgctgcaa gctgatcgac
     1441 ttgggaaatt gggcgtccgg ccaatacgtg atctcgacgc cgatccggcc ggattccgaa
     1501 caaaaaccgc ccgagggcga gagcgatggg gatggcgagt agcggccggc cgccggcggc
     1561 aatctggctg gccacgtggg gaggctgtgg gtatttcccc aaagggcctg gaaccgcggg
     1621 gtcgctcggc ggggcagccg tggcgtgggt gctggtcaga tgggcaggtt ggcccccggc
     1681 cgcactcgct gcggcggccc ttggccttct cggtccggga gtctgggcat cgtcccgggc
     1741 ggctcgttat tgggggcagg aagatcccca gcgggtggtg atcgacgaag tccttgggca
     1801 atggctggcg ctgtccgtgg cgccggcggc ggagtggcga tattggctcg cggcgttcgc
     1861 attatttcgt atatttgata tttggaagcc gtttccggtg cgctccgccg agcaattagc
     1921 cgccggctgg ggcatcgttg ccgacgacct ggtggcgggc gtctatgccg ccgttgttct
     1981 gctggcgctg cggcggttac acttttagct agcaagcttt gctggaagtt atgggtcttg
     2041 ttcggaagga aggcggccag cggccgcaaa tctcgagcgt gaaaccggcc tcggcgattg
     2101 ccggagggga atttgtcgtc caaggcgccc ggtttcgggc caatggcgag gagcgtcctc
     2161 gcgtccgctt tggagaggtg gaagcgccgc tagtggtggg agccgatgat ttcatggtgg
     2221 tacgcgttcc tgaaggcgcc acggtgggcg aactgattat tgaaaatcga gacgcctcca
     2281 gctccccata tgcctgcaac ctcggatttc ccattgccga aaacctccac ccggtagcca
     2341 acccggtcgt ggatatgcac ggcaatattt acaccacgtt cagcggctcg cgcggccaga
     2401 aggtgccggt ttcggtttac aaaatcgatc cgaactacga gatgaagccc ttcgtcaaag
     2461 atctgatgaa cgccacgggg ctggctctcg atcgcgaggg catgctgctg gtttccagcc
     2521 ggttcgacgg aaccatttac caggtcgcgc ccagcggcaa gatgagtatt tattcggagg
     2581 gaatgggaat cgccaccggc ctcgcgctcg atgaggaagg caatctttac gtcggggatc
     2641 gcagcggcac gattttcaag attgccccca gcctggcgcc ggcgcggcaa atttttgtgt
     2701 tcgctacgct ggagccctcc atttccgcct accatcttgc agtcgctcct gatcagagcc
     2761 tgtttgtgac cggtcccacg acgtccagtt tcgattccat ctaccgcatt tcccataacg
     2821 gcgaggtgga ggtgtattac cgcggtttgg gccggcccca gggcatggct ttcgacgcgg
     2881 aggggcgcct ttacgtggcg gcgtccctgg gaggccggaa agggatctgg cgctttgggc
     2941 cggagcgcaa acccgaactg gtgctctccg ggcctggcat tgtcggcctg gcgttcgcct
     3001 cagcgcacac tgccatcgtt gccaccaata acgctctcta tcgggtggac cttgctccct
     3061 aaattgcgcc cgctttgatc gattcctcca ttgacgattc aagacggcgc tgaaacaata
     3121 atcggtttta tgcaatgggc ggctgcgtgg gatggccggc cgcggaatcg ccgatgacag
     3181 ccgaaatcct ggccgtaggc tccgaactgt tgacgccgca gcgcgtggac actaattctt
     3241 tgtatctaac ccagcggcta aacgatctgg gaatcgaagt tgtatcgaag gcggtcgccg
     3301 gagatgatcg cgtgcggctg gccctgctcc tcgggcaaat gcgcgaacgg accgaactgg
     3361 tgattgtcac cggggggctc ggcccgactg aggatgacgt tactcgcgac gcggctgctc
     3421 aggtgtgcgg gcgcgagctc gtcttccgcc aggaggtgct ggaccagatc gaagcgcggt
     3481 ttcgctcgct tcgccggact atggcgccca tcaatcgccg gcaagcgtat atcatcgacg
     3541 gcgccgagat tctacccaac cagcatggaa cagctccggg ccagtggtat aaagatgccg
     3601 ccggcatact gattctcctg ccggggccgc ctaacgagtt gaagccgatg tttgagtcgg
     3661 aatgcgtccc cagactgcgg ctggcggctc ccgagcaata tatcgcgacc ttcatgatgc
     3721 gcgtcgccgg gatgccggag tctgacctgg atcagctcat ttcgccgatt tacacccgct
     3781 atactaatcc ggtcactacg attcttgccg cgccgggaga cattcaaatc cacttgcggg
     3841 cccgcggagc cagcgaatcg gaggcccggg tcgtggtcga ggagctggcc agacagatcg
     3901 aggcggtttt gggcaaccgg gtgtattcgc ggagcgcggc gactctggaa gaagtcgtgg
     3961 ggcgcatcct taccgagcgc aacgccaccc tggcggtggc cgagagctgc acgggcgggc
     4021 tgatcgccga gcgaattact tcggtgccgg gcagctctcg gtattttgca gggggctgga
     4081 ttccctactc gaatcgcgcc aagagggagt ggctgggggt cagccaggaa acgttagaaa
     4141 cgcaaggcgc cgtcagcgcg caggtggctc gggagatggc tcaaggggcg cgcgcgcggg
     4201 cttgcagttc tctgggattg tcagtgacgg gcgtcgccgg cccgactgcc gacgccggcg
     4261 gaggggaatc cggccccaaa cccgtcggct tggtttacat cggtctggcg gatgaggcgg
     4321 gctgcgaagt gaaggaaagg cagtttctcg gcgaccgcga ccggatccgc gcgcaggcca
     4381 gccaaatggc tctcgacatg gtccgccgca gatttctggc gtaaatcggg cggcgatata
     4441 ctggtgttgg aatcgctcac agcagcattg ttgcgggcct ccggcgagcc ggccgccccc
     4501 gggacccgcc gaccgcgccg tatgccgcca ggcgaagcgc ccgccagggc cgaggaggga
     4561 gagtgaaagc gttaggtatt attgctgccg tcgtggtgct ggcgggagtg gcgctgagtt
     4621 cctcgtttgt cagcaggcgc aacgagatgg tcgcgcagcg ggaagccatc aagaaccagt
     4681 gggcgcaggt ggatacggcg ttgcaacggc gcgccgacct gattcccaat ctggtcgaga
     4741 ccgtcaaagg ctatgcctcg caggagcgag ccgtatttga gagcatcgcc caggcccgcg
     4801 ccgctatggc gggcgcaaag acgcccgccg agcgcattgc cgccaacgat caattgagcg
     4861 gcgcgctttc gcgattgctt gtcgtggtgg aaaattatcc gcagttgaaa tccaacgaga
     4921 acttcctgcg cttgcaagat gagctcgcgg gaaccgagaa tcgcatcgcg gtcgagcgcc
     4981 gcaagtacaa tgaggtcgtg caggcctata acacgaacat ccagttgttc cccaacaaca
     5041 tcgttgctgc gatctcgggc ttccagcgcg aagaggccta tttccgggcg agcgacagcg
     5101 cccgccaggc gccagcggta aagttctgag atccctccgc gcggcgcgac gctacgccgg
     5161 cgggcttctc gggccgcctt tcattcagcg ggtttacgaa cggctcagcg atttgagcat
     5221 cgcctgaaat gcgccctgat tcgccgctac tgtttttgcc ggccccgtga acttgaaaaa
     5281 aaccggtccc cgcggaactt ctacgatggc gccaagcagt cggtagcccg gtttgcggat
     5341 cacttgctgc gccatggggc cgccggcccc cgtgtaggtc ccgctggcat cgacggtcgt
     5401 cactcgcagg ccgttgatgg tttgagtgtg gatctgagca gactgccggg atggcttgcc
     5461 gccaggctgc tcgaactggt cgacccagcg gttcacgtta tcttcaaccg tgccgccttg
     5521 cccggggccg aaataaaata caccgcactc gcccgcctcc ttatcgcccg gcgcggccgg
     5581 tatttcgtag gtggccgccc gcatcggtct ggctccttgc gatttccacc cggcgggcat
     5641 cgtccatttg acgccgccgg cggcgtcggc gagcaacagc gcgctcgcca gcgcggagag
     5701 cgccagaagt tttgttctca taggctcagt ataacgaggg cggctgggcg ccagggcgta
     5761 gccgagcgtg gccggctcgc cgcgagtgct atactagcgc tcttctatgc tcgaacgcta
     5821 tcgcgccgct ctctcgtggc tggcgcactc cggcatccaa gccgaatccg gcggtttggc
     5881 gcgctactat cgcgccgaag ccggagaata caaagacatt tccactgaga tcaccgctta
     5941 cggcattcag gcttaccttc agttgccatt gcccggggaa agcggtcttc tctcgaacgc
     6001 tttgcgcgcg ggacagtttc tttgctatga cgcgaccgat ccgcagacgg ggctgctgtt
     6061 tttcgagttg tcgccgaacg gatcgcccgc gagccggcta gcttattttt ttgattgcgc
     6121 catcatcatc cgcagcctca ttgcgctctg gaacacgacg gccgacccca tgtatctgga
     6181 gcgcgccgag cgttgcggtg cgggaatgat gggccgcatg tcgcgcgtgg acggctcgtt
     6241 ttttccgatg ctggatctcg aaaccggcat ccccagcagc gctcccggag gctggtcgct
     6301 cgaggccggc ccctatcagc tcaaagccgg cctcagcttc attgaactgg ccgaagtaac
     6361 cagttccggc ctgttttcgc gaaccgcaga cgcgctgctg gactggtgcc tccgccagca
     6421 cgaactgttt cttcgcggcg aagaccgtcc ggaccgcatg gccgaccgcc tgcatgccta
     6481 ttgctatttc ttagagggac tgctgccttt cgtggaaaag cggttcgaat gcacgctggt
     6541 tttgcaagcg ggcatcaatc gcgtcgagac cctggcggcc gaggcgagtg cggctctgga
     6601 acgctgcgat gtgttggccc agcttctgcg cttgcggctc ctgtgcgatt acatgggcct
     6661 gctcgagctg gacgcttcgg cggcgagccg cgaggcggaa gcgctgctcg ccttccagtt
     6721 gcacacggac gaccgccgga ccaacggtgc gttcgccttc ggccgccgcg acggccagct
     6781 ttcctgccat gccaacccgg cggcgacgat cttcgctctc caggctctgc ggatgtggaa
     6841 ggagcaccag agcggcgagc tgcggatgag ctggcacgac ctgatctgat gcgcccgcga
     6901 aaggacctat tatgcccgat tttgatctga tcccgaggcc ggtcccggcc gtagaaacca
     6961 aataccgcag aattgtcacg cccattcccg tgccggagtc gattcctgtc ctcgagcggc
     7021 ttttcgcctg cgagccagtg gcgatgcgag gccaaccgcc gattgtgtgg gaccacgccg
     7081 agggcattac cgtgcacgac gcctggggca accgatggct ggactggtct tcgggcgtgc
     7141 tcatcaccaa tgccgggcac ggccgcccgg agatagcggc ggcgattcac gaccaggtca
     7201 atcatcaact gctgacaaac tattgcttcc caagcgaaat ccgcgcccga ctggtggaaa
     7261 agctcgccgg gctgctgccg gagccgctga agaagatctt tctgctgacc acgggatcgg
     7321 agaccgtgga atgcgccgtc aagctgtgcc gcacgcatgg gatcaaggcc ggcggccgcc
     7381 gaaaaatcac gattgtctca tttcacaatg catttcacgg ccgcacgctc ggctcacaac
     7441 tggccggcgg catccccgcg ttgaaagatt ggattgtcaa tctcgacccc gatttcgtgc
     7501 aggttccgtt tcccgacggt taccgctgcc gggacacttc cttccagcgc ttcgagcgct
     7561 cgctggacga ggcccgggtg aaaccggaac gcgtagccgg cgtggttctc gagacgtatc
     7621 aaggcggcga cgccgccttt gccccgcctg cctacatgca ggagctgcgc cgctggtgcg
     7681 accggcatgc ggcgctgctg gtgtgcgacg aagtgcaggc aggcttcggc cgaaccggga
     7741 cgctgttcgg cttcgagcat tacggaatcg tgccggacct ggcctgcttc ggcaaaggta
     7801 ttaccagttc tctgccccta tcggcggtag ccggccgtcc ggacgtcatg gacttgcacc
     7861 caaaaggcag tatgacttcc acccacaccg gcaatccggt ttgctgcgct gcggccctgg
     7921 ccaatatcga tgtgatcctg aaagaagacc tggccggcaa cgcgcggcgc gtgggcgaga
     7981 cgctgcatga aagcctgcga gcgctgaaag ccgactttcc gcagattgcc gcggtgatgg
     8041 gcaagggtct ggtggccgga ctgccggtta tcgacccgct tacgaaggag ccggattcgc
     8101 atctcgctta cgacgctgtc cggcgctgct atgaaaaggg cctgctgatg ttcaacccgg
     8161 tgggcttcgg cggcgccacg atcaagatag ctccgcctct caccaccaac gccgaggccg
     8221 cggtcgaagg cgtcagcgtg ctgcgcgagg cgctggcgga ggccatcgca gcgaggcaaa
     8281 gcacgctgca agaggtcgtc ggagggtgag cgcgccggcc gtcgtcttaa ttggcgcggg
     8341 catgatcgcc cacgaccaaa tcctgccgtc tctctaccat atgcagcggc aaggcctcgt
     8401 cggtgagatc agcgtctgcg cttcgcactc cggcgccttg aaaagactgg cgggagcttc
     8461 cattttccgg cgcgcttttc ccgggcaatc gttccgccct tatccggatt tttctgctga
     8521 tcctgacgag ccgcacccga atctttttcg cgaagtcatc gaaaaaatgc aggcgggcgg
     8581 catcgcggta gtggccgtac ccgatccgct ccatttcgac gtgatcatgg ccgcgctgcg
     8641 agccgggctg catgtgtgct cggtgaagcc gcttgtgctg acggcccgcg agtccgttgt
     8701 gatcgagcgg gaggctttcg ccaggggatt agtggtaggc gtggaatatc acaagagatt
     8761 cgacgaccgc agcctcctgg cgcggcgcaa ataccgggaa ggggctttcg gcgaatttcg
     8821 cctcgggtcg gcttgcctgc tcgagaaatg gtattaccgc cattccaatt ttcaaaactg
     8881 gatgaccacg gagcactcgg acgccttcac gtacatcggc tgtcactacg tggacctggt
     8941 gcactttatc accggcctgc tgcccgaagc ggtgagcgtt tacggcattc gcgaccggtt
     9001 tccaaacgga aaggagggct ttctgtggac agatgcccga gtgatctggt ccaacggcgc
     9061 ctgtctgaat gtccagaacg ctctcggttt cccggatgcg gcccccggga cgaacaccca
     9121 ggggctgacg ctctattgcg ccagcggtga taccggaacg ctgatttccc attcggatca
     9181 ataccgcggg ttgaaatact cttacgcgag caagccggac gaaccggggg ccgcgcagta
     9241 cgccgaaccc agccccgatt attttcagta tgtggacctc ggcggcccag gcctgacgcc
     9301 ggtcggctac ggctaccgct cgatcgagta catcctgcgc gcttgcctcc gcgccggcgc
     9361 cgaaacgagt ctggcgaagc ggcaggagct tctgaaagag tgggacgaag ccggtatcat
     9421 ggcgaccccg gccaacagcc gctacaacga gcaggtcatt gaagcggcgc gagaatccat
     9481 ccggaatcgc ggccgcgagg cgccgataaa acgcgatccg ccggcagcct aagaaacggg
     9541 ctcccaagcg ccgctgcgcc tttgccgagc cggccgaaaa actgctaacc tgtcgctaca
     9601 caagaccggt ctggaggtgt tccatggctt gcacgcggcg aaatgtcctc agttcgatgc
     9661 tttcggcggc gggaattgcg ttcgctcaac gtcccccgcg cccg
//
LOCUS       DASREK010000072         5415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1858933, whole genome shotgun sequence.
ACCESSION   DASREK010000072 DASREK010000000
VERSION     DASREK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858933"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VFA54_03850"
     CDS             <1..1977
                     /locus_tag="VFA54_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS49969.1"
                     /translation="TAISTSATYVKSPTFIVDATWGVTNPHQLLFPTITDQRYGSDVL
                     GIPGTNIGPLPWAGGLPAFGIANYGASGVGSITYGYSYPALEYKQPVIEYVVNATKIH
                     GSHNIRFGMDIARLHANHIEVSPSGFFFSGGVTAVRGGPSPTPYNSVADFILGLAQSE
                     QNFIQVVQPYLTLRVWQDSLYIRDQWQVNRKLTVNYGVRWEYYPVPTEANRGIFFYDI
                     PNNQVEICGMGSVPSACGIHVSKKIFAPSIGLAYRPFEKTVIRAGFATSPFQIDISRS
                     GMKSYPDEIGAQVNGPNAFEPAGSVSQGIPVIPTPVFVDGKITPPPGTGNLYTTPLNI
                     DRGYIESWNLTVQREFRGGFTGSVGYVGTHDVKLNSSYNVNYGQVGGGTASQPFFSRG
                     ITGSVNVLLPVGSAHYHSLQTTLQRRFANGLSLQAAYTWSHEIGMCCSETGSPQILIP
                     QYQNLNRATMPLDVTHNFHLATVYELPFGKNKPLLKSGFAAIIAGGWSTNAILGHVSG
                     LPFNVTASSASLNAPGSSQRADLVKPSVAIVGNGLGGQAYFDPLAFAQVTTARFGTAG
                     WDILRGPGNTNLDLSLFRSFPINERFKLQFRVESFNLTNTPHFGNPGANVSNLQVRSD
                     GTLNLNGFSQITTQVPLGRLIDPRYFRFGMRVMF"
     gene            2417..3370
                     /locus_tag="VFA54_03855"
     CDS             2417..3370
                     /locus_tag="VFA54_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS49970.1"
                     /translation="MRNTKKRLGWLLLVLGLVAAHVTAVAQTPKPKRINKVIELWEQG
                     QPVYFNGPHEGIAGSYEQGLKDAQTYLDYISYDMEHAPFDVKGLAEYMRGLAAGGPTK
                     SGHRTPAVIVNVPVNGTDEATVRANAWMFQQVLATGVHGILLCHADTAGAVRAFVEAV
                     RLPIHKQGVDKGLNEGRRGAHGSTTAARIWGITPEEYNQKADVWPLNPNGELVLGLKL
                     EDKYALANAEEIVKVPGIAFAEGGPGDMALSLNVKASDPRLEEAEARLAKICLANKII
                     FMHGYTPENVIDVIKQGFLVGRSGPEAAEIGRRYTKRQMPW"
     gene            4020..4703
                     /locus_tag="VFA54_03860"
     CDS             4020..4703
                     /locus_tag="VFA54_03860"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS49971.1"
                     /translation="MLALAVGLAAALPLSGQTFGQITGLVTDPSGGVVVKAAVTVTNP
                     QTNFTRTENTNAAGVYTFPNLLPGVYSIKVEMPGFQAEVRNNVELQVQQVARLDFQLQ
                     VGAVAQTVEVSTGAPLLNTEDATVGTVIENQRIVDLPLNSRNFLQLVALSPNVTAGFA
                     NGGQSSSRLGGDRSTQQLSISGNRREWNYFTLDGMSNTEVNFNTYLFVLADLDRLRPG
                     RGNRHQPEA"
BASE COUNT         1095 a         1638 c         1613 g         1069 t
ORIGIN      
        1 acggcgatct ccacgtcggc cacgtacgtg aagagcccca ctttcatcgt tgacgccacg
       61 tggggcgtaa ccaatcccca ccagttgctg tttcccacga tcaccgatca gcggtatggt
      121 tcggacgtgc tgggaattcc cggcaccaac ataggacctc tcccctgggc gggcggccta
      181 ccggcgtttg gcatcgccaa ctatggggcg agcggagtgg gcagcattac ctacggctac
      241 tcgtacccgg ccctcgagta caaacagccg gtgatcgagt atgtggttaa cgcaaccaag
      301 attcacggct cgcacaacat tcgattcggc atggatatcg cgcgcctgca tgccaatcac
      361 atcgaagtat cgccgagcgg cttcttcttt tccggcggcg tgacggcggt gcgaggcggc
      421 cccagcccga cgccttacaa ctcggtagcg gattttatcc tgggcctggc gcaaagcgag
      481 caaaatttca ttcaagtcgt tcagccctat ctcacgctgc gcgtctggca ggacagcctc
      541 tacatccgcg accagtggca ggtgaaccgc aagctcacgg tcaactatgg cgtgcgctgg
      601 gagtactatc cggttcccac ggaggcaaac cggggcattt tcttctatga cattccgaat
      661 aaccaggtcg aaatctgcgg catgggctcg gtgccgtcgg cctgcggaat ccacgtgtcg
      721 aagaagatat ttgctccgag catcggtctt gcctaccggc ctttcgagaa gacagtcatc
      781 cgggcgggct ttgccacctc cccgttccag attgacattt cgcgcagcgg gatgaagagc
      841 tatccggacg agatcggcgc ccaggtaaac ggacccaatg cgtttgaacc ggcgggctcg
      901 gtcagccagg gcatcccggt gattcctacg ccggtctttg tggacggcaa gattactccg
      961 ccccccggga ctggcaacct ctacacgacc ccgttgaata ttgaccgcgg ctatatcgaa
     1021 tcctggaatc tgaccgtgca acgggagttt aggggcggct tcacgggctc ggtagggtat
     1081 gtaggcacgc acgatgtcaa gttgaattcc tcgtataacg taaactacgg ccaagtggga
     1141 ggcggcacgg ccagccagcc tttcttttcc agggggatca ccggttcggt gaacgttctg
     1201 ctgccggtcg gctctgctca ttaccactcg ctccagacca ctttgcaaag gcgattcgcg
     1261 aacggcttat cgttgcaggc agcctacacc tggtcgcatg agatcgggat gtgctgtagc
     1321 gaaacgggct cgccgcaaat cctcattccg cagtaccaga atctcaaccg cgccacgatg
     1381 cccctggacg tcacacacaa cttccatttg gctacggttt atgagctacc cttcggcaaa
     1441 aacaagccgc tgctgaaaag cggcttcgca gcgattatcg ccggcggctg gagcaccaac
     1501 gcaattctgg gccatgtctc cggattgcct ttcaacgtga ctgcttccag cgcttctctg
     1561 aacgctccgg gcagcagcca gcgcgccgat ctggtgaaac cgagcgttgc catcgtcggc
     1621 aacgggctcg gcggccaggc gtacttcgac ccgctggcgt ttgcgcaagt caccacagcc
     1681 cgcttcggaa cggccggctg ggacatcctt cgggggccgg gcaatacaaa cctcgatttg
     1741 agcttgttcc ggagcttccc gatcaacgag cgcttcaaac tccagttccg ggtcgagtcg
     1801 ttcaatctca ccaatacgcc acacttcgga aacccgggag cgaacgtctc caacctgcaa
     1861 gtcagaagcg acggcacgct gaatctgaac gggttcagcc agattaccac acaggttcca
     1921 ctgggccgtc tgatcgaccc gcggtacttc cgcttcggca tgcgggtcat gttctgatag
     1981 ctggtgaggc tggccacagg catgccggcc ggctctatct cttttcgaag gcccggagct
     2041 ccaggcccgg gccttttgtt ttggctggac cggcgcacgc gccgccggca gctcggctgg
     2101 cctgcgctca accaattcga cctacgtccc ggaaaatcag gctgcgcgac tccgggcggc
     2161 tgaaagccga cttcccgggc ggcattttcc tggtttcccg gttcagcgtt tcgtattatc
     2221 atgccgagta ccggattatg cccaataggc gcagtgttcg gtccgttggt ttcgcatcgg
     2281 cacagttccc ggcgcttccg ttgaaaaaaa cagtgatccc gacctggtcc cgccggaacg
     2341 ctagcgcgcg attttgcttt ggagccagaa gaacagagcg agcggagcgg acattgtctg
     2401 gaggaggaga gcatccatga ggaatacgaa aaagcgcctc ggttggctgc ttttagtcct
     2461 gggtctggtt gccgcgcacg ttacggccgt ggcccagaca ccaaaaccaa aacggatcaa
     2521 caaggtgatc gagctctggg agcagggcca gccggtgtac ttcaatggtc cccacgaagg
     2581 catagcgggg agctatgaac aggggttgaa agacgcgcaa acgtatctgg attacatcag
     2641 ctacgacatg gagcacgctc cgttcgacgt caaggggctg gccgagtaca tgcgcggact
     2701 ggcggcgggc gggccgacta agagcgggca tcgcacgccg gctgtcatcg tcaacgttcc
     2761 ggtcaacggc acggatgaag ctaccgtgcg agccaacgcc tggatgttcc agcaggtgct
     2821 tgctaccggc gttcacggca tcctgctgtg ccatgcggac actgcgggag cggtccgggc
     2881 ttttgtggaa gccgtgcggc tgccgatcca caagcaggga gtcgacaaag ggctgaatga
     2941 aggacggcgg ggcgctcacg gttccaccac agccgccagg atctggggaa tcacgccgga
     3001 agagtacaac cagaaagccg atgtttggcc gctgaatccg aatggcgaac tcgtgctcgg
     3061 cctcaaactg gaagacaaat atgccttggc gaatgccgaa gaaatcgtca aggttcccgg
     3121 catcgctttc gccgaagggg gccctggaga catggccttg tctctcaacg tcaaggcttc
     3181 ggatccccgg ctggaagagg ctgaggccag gctcgccaag atctgcctgg ccaacaagat
     3241 tatcttcatg catgggtaca ctcccgagaa cgtgatcgat gtcatcaagc agggatttct
     3301 ggttggccgc tccggcccgg aagccgcgga gatcggccgc aggtatacca agcgccaaat
     3361 gccttggtga gacataagcg ccgccgcgcc tatgattgcg cgctttgggc cgccgtcgag
     3421 cctgcgctcg ccgggtggaa aaaccgccct tggccgctgc ggccgggagc agccgagctt
     3481 ccggcatcgg cccgcaaggg cattgcttgt tttttcgcac cggcgggcct gcaaacccgc
     3541 cttctcttgc cggcgcctca aagcgcagcc ggaggtctgg ccggcttggc ggccgcgatc
     3601 actcacgggc aaggcgcgcc gagaaatccc gcgtccattc gcgtgcagag gccgttgatg
     3661 ctggcgtttc acgttgacga actcgagtca ccagtgcgtt cacattcagc aactctcagg
     3721 gccgattcca gttgacacgg tgaagcgttt ccgatacgat cagccagatt cccgagagct
     3781 tggaagcgaa gggggagtga gattgtgcgt cagttttccc tcgctggggt tctcagagcg
     3841 ttcaatgccg ccgtgcgcgc ggtgcaattt tttccaattc cggctattct tacggggtcc
     3901 aatcgaaatc gggtcttccg gattttttcc ccttctcggg ccgtcctcaa accggctcta
     3961 cgaaatctca acaatttcaa caaagggggt acgcgccgat gcgcacaagg cttggtggga
     4021 tgttggctct ggctgtgggc ctggccgccg ctctgcccct gtccgggcag acattcggcc
     4081 aaatcaccgg gctggttacc gacccgagcg gcggggtcgt cgtcaaggct gcggtaacgg
     4141 tcacgaaccc gcagaccaac ttcacccgga cagaaaatac caacgccgca ggcgtttaca
     4201 cgtttcccaa tttgctgccg ggcgtctaca gcatcaaggt ggagatgccc ggctttcagg
     4261 ctgaggtgcg caacaacgtt gagctgcaag tgcagcaagt ggcccgtctc gattttcagc
     4321 ttcaggttgg cgcggtggcc cagaccgtag aagtgagcac gggcgccccg ctgcttaaca
     4381 cggaagacgc tacggtggga acggtaatcg agaaccagcg gattgtggac ctgccgctca
     4441 acagccggaa ctttctccag ttagtggctc tcagccccaa tgtgacagcc ggctttgcca
     4501 atgggggcca atccagctcg cgcctggggg gcgaccgcag cacgcagcag ctctccatat
     4561 ccggaaaccg acgcgagtgg aactacttca ccctggacgg catgagcaat accgaagtca
     4621 acttcaacac ttatcttttc gtgctcgcag acctcgaccg gctacgaccg ggccggggca
     4681 accggcatca gcccgaagct tgacaatcca agcccgggcc agtggttcaa tatcgccgcg
     4741 ttttcgcttc cagcgctgaa cacgtatgga aactcgggcc gggacgtagt cattggtccg
     4801 ggtattttcg actgggattt ctccacgctg aaaaacttct acttcaccga aaaaagatat
     4861 attcagttcc ggttcgaatc gtttaatttt ctcaaccacc cgaatttcgg ggatcccaac
     4921 ctgaatttgg ggaacagccg ggtggactcc aagggcgtgc cgattgcagg aagcggcgcc
     4981 ttcgggacca tctcgtcgac gcgttccggg attgacatgc gggaattgca gttcagcttg
     5041 aaattgattt tttaatcggg gaaggctggc gattgggagt gggctctcgc ccggcccttt
     5101 ctcgagagcg cgccatgccg gcagcacacg ggcccggcgg ccgcttgccg ggaggggatc
     5161 gcggcggaac tcaaaaactg agagcggcgg cccccttccc ggcgcgcacg gcaatgcgcc
     5221 ggccagaaaa caaacgcgat tcctccccgc ccttcgaaac accgaaggcg gggagcctgg
     5281 ggcattctcc gttacggttc gagcgtaaac gcgtacagcg aatcgccggc cccaaccacc
     5341 agatactgct ggccgtcgag catataggtg atcgggccgt tgctgacgtt ggccgtcaag
     5401 ccggcatgcc aaaga
//
LOCUS       DASREK010000073         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_209774, whole genome shotgun sequence.
ACCESSION   DASREK010000073 DASREK010000000
VERSION     DASREK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209774"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFA54_03865"
     CDS             complement(<1..325)
                     /locus_tag="VFA54_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49972.1"
                     /translation="MKPINWRDFPLIALAAVFTAGALYAQEHNAKQSHSAASVPEVFC
                     SHMKTGQLCSGNASMFKLSDSQKQQYREALSSYNKAVDAAQKQFLADLKDKVHLSETE
                     LALAES"
     gene            complement(361..1350)
                     /locus_tag="VFA54_03870"
     CDS             complement(361..1350)
                     /locus_tag="VFA54_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HZS49973.1"
                     /translation="MKIEVLYFDGCPHHRPAVERVRRALREEGLSAEVEEINVPDAAS
                     AQALGFLGSPTIRIDGRDIEPAARASASFGLMCRTYREGGVPAGVPPMELIRAALHEA
                     AGPQQSASRCCESPSAGAISSESAAPQRQRLLLISAVAAAIAASLCCILPIVAAATGL
                     GAIAVGAAFEKWRPYLLGVAGLLLAGGFLVAYRDRKTACAPGSVCATKPVRRWSLIAL
                     GLAAALAVGLAAFPYYSGAVAKAVLGAPGSVHSAASSRVVKAAFRVPDMDCPACAVSL
                     SAALRKLPGVLDVNLNVDTHRAVVTYDPASQNVAVLERAITGAGFHPSPEPRS"
     gene            complement(1365..1790)
                     /locus_tag="VFA54_03875"
     CDS             complement(1365..1790)
                     /locus_tag="VFA54_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HZS49974.1"
                     /translation="MSAVAKIGQAAREAGVSIDTIRFYEKQGLLKSFGRTQGGFRLFG
                     EQEIEELKFIQKAQELGFSLAEVRELLLLQRDGMEACEHVRDLLEQKIAQVRSKVEEL
                     RKLEVSLKRALRQCERNLDESAREHECCPVLEKLGRKGA"
     gene            complement(2081..>2588)
                     /locus_tag="VFA54_03880"
     CDS             complement(2081..>2588)
                     /locus_tag="VFA54_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS49975.1"
                     /translation="HPITILAGLPSAGLGALVTLWLFGNELNVYSFVGLVMLIGIVKK
                     NAIMQIDFALEAERKEGKAPSDAIYEGCLIRFRPIMMTTMAALLGALPISLGYGAGGE
                     ARRPLGLTVVGGLMFSQLITLYLTPVVYTYMASVVERWNARKHGRVHAKTPSRPRGSF
                     EPAEAKGD"
BASE COUNT          503 a          837 c          753 g          495 t
ORIGIN      
        1 agctctcggc cagcgccagt tcagtctccg acaaatgaac cttatctttg aggtccgcca
       61 gaaactgttt ctgcgccgcg tccacggctt tgttgtagct gctgagcgct tcccgatatt
      121 gttgcttttg cgaatcgctc agcttgaaca tgctggcatt gcccgaacag agctgcccgg
      181 tcttcatgtg cgaacagaag acctcgggca cgctggcagc gctgtgcgac tgtttggcgt
      241 tgtgctcttg cgcgtagagg gcgccagcgg tgaagacagc cgccagcgcg atcaggggga
      301 agtctctcca atttataggc ttcatgtctt tctatctcct ccttcgcaat gaatggatcc
      361 tcaagagcgc ggctccgggc tggggtggaa gcccgcgccg gtgatggctc tctccaacac
      421 cgccacgttc tgcgacgccg gatcataggt caccacggcc ctgtgagtgt caacattcag
      481 attcacgtcc aaaacgccgg gaagcttgcg gagcgcggcg gaaaggctga ccgcgcaggc
      541 cgggcaatcc atgtccggaa cgcggaatgc tgccttaacc acacggctgg aggctgcgga
      601 gtgaacggat ccgggcgctc ccagcactgc cttggcaact gcgcccgagt agtaaggaaa
      661 agcggctaaa ccaacagcca gagctgcggc caaccctagg gcaatcaggc tccagcggcg
      721 gacgggcttc gtggcgcaga cgctgccggg agcgcaggcc gtcttccgat cgcgataggc
      781 cacaagaaag ccgcccgcca gcagcagccc cgcgacgccc agcaggtagg gccgccattt
      841 ttcaaacgcg gcgccaaccg cgatcgctcc caggcctgtc gccgcagcca cgatgggcag
      901 aatgcagcac agcgacgccg caatggccgc cgcgaccgct gatatcagaa ggaggcgctg
      961 ccgctgggga gcggccgatt ccgaactgat cgcccccgca ctcggactct cacaacaccg
     1021 gctggcgctc tgctgcggtc ccgcagcttc gtgaagagcc gcccggatca gctccatcgg
     1081 tggaactcct gccggaacgc ctccctccct gtacgtccgg cacatcaagc cgaagctcgc
     1141 cgaggcccgg gcggccggct cgatgtccct gccatcgata cgaatcgtcg gcgagcccag
     1201 gaatccaagc gcctgcgcgc tcgccgcatc cggcacattg atttcttcaa cctccgcgga
     1261 gagaccttcc tcccgtagcg ccctcctgac ccgttccacg gctgggcgat ggtgagggca
     1321 cccgtcaaaa taaagaactt cgattttcat cggtcgattc tccttcacgc gcccttccgg
     1381 ccgagcttct ccagcacagg acagcattcg tgttctctcg cagactcatc aaggtttcgt
     1441 tcacactggc gcagggcccg tttcaggctc acttcaagtt tccgtaactc ttcgaccttg
     1501 ctccgcacct gtgcaatctt ttgttcgagc aggtcgcgaa cgtgctcgca ggcttccatc
     1561 ccgtctcttt gcaggaggag cagttcgcga acctctgcga gcgaaaaacc gagttcctga
     1621 gctttttgga tgaacttgag ttcctcgatt tcctgttccc caaacaggcg gaatccgccc
     1681 tgagtccggc cgaaactctt cagcaatcct tgcttctcat agaaccggat ggtatcgatg
     1741 cttacaccag cctctcgcgc cgcctgaccg atctttgcca ctgcgctcat ctgcgttttt
     1801 gttcccatat ccagaataca atctagagtt gactccagag tcaagtgaaa aaatagcgag
     1861 tacgccactc tggggacagg gggtgcgccc tcaaattgtg tggccgattt tccgcgcagc
     1921 agcaccgccg agactcacgg ccgcctccgg aggaaccggc gctcagcttt ttgatacgtg
     1981 gcaggggcat gccaacgcgg ggcaggcggg ctgcggctcg gggtactcgg gcgctttctt
     2041 ggcggcgcaa gaaaacggga aggatctaca gagtaagatc tcaatcccct ttcgcttcgg
     2101 ccggctcgaa ggagccgcgg ggcctcgacg gagttttggc atgcacgcgc ccgtgcttgc
     2161 gggcgttcca gcgctcgacc acggaggcca tataggtata gaccaccggg gtcaggtaga
     2221 gcgtgattaa ctgtgaaaac atcaggccgc ccacaaccgt cagccccagc ggccggcggg
     2281 cctcgccgcc ggcgccgtag ccgagcgaaa tgggcagtgc gccgagcagg gccgccattg
     2341 ttgtcatcat gatcggccgg aaccggatca ggcagccctc ataaatggca tccgacgggg
     2401 ccttgccttc tttccgctcg gcttccaggg cgaaatcgat ctgcatgatg gcgttcttct
     2461 tcacgatgcc gatcaacatc accaggccca cgaaagagta cacgttcagc tcgttgccga
     2521 acagccacaa cgtgaccaac gcgccgagtc cggcagaggg aaggcccgcc agaatcgtga
     2581 tcgggtgg
//
LOCUS       DASREK010000074        14823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1364420, whole genome shotgun sequence.
ACCESSION   DASREK010000074 DASREK010000000
VERSION     DASREK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364420"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFA54_03885"
     CDS             <1..825
                     /locus_tag="VFA54_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZS49976.1"
                     /translation="ARQEGFTSMGIWANRRTPLDPETATDAAVARIQEMLRRSGLHIS
                     VIGCSESHIDPDPQARARANSYFGKVIELAGRLGAPHVGTQTGGMPGRPLAEQVDELV
                     RVYTEKYFPLCQKHHVRILWEPYAGRPNIATGPVGYEALFKAFGDSPYIGLQYDPSHL
                     VWQMMDPIQCARDFVDKIYDVHLKDTEIRWPILRKTGIHPLNNAQWWRFRLPGLGQID
                     WAAFFTVLMDAGYSGAMNIEHEDPLYGHPNQGVDFTDDYKTGFRVAHQYLRQYVPA"
     gene            complement(867..2732)
                     /gene="glmS"
                     /locus_tag="VFA54_03890"
     CDS             complement(867..2732)
                     /gene="glmS"
                     /locus_tag="VFA54_03890"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZS49977.1"
                     /translation="MCGIVGYVGSRKVVPLILDGLRRLEYRGYDSAGIAVAGEHNGLM
                     VRRSSGKLRNLEEAIRINPMDGSYGIGHTRWATHGRPTEENAHPHRDCTGDIVVVHNG
                     IIENFLALKKQLIGEGHHFTTETDTEVIAHLIEKHYNGKAAGNLENAARAAVRELGGV
                     FALAIISRRDPNKIVAARCGPPAVIGLGDNEYFVASDVSAILHYTRDIFFLMDGDMAV
                     LTPEGVRLTDFEGRPVNRQVQHILWDPVMAEKGGYKHFMLKEIFEQPRAVRDTTIGRV
                     SRETGKIYLDEMDIAPSEFSSFRQVKIVACGTSWHAALAGKFMIERLARLPVEVDYGS
                     EFRYRDPIIQPETLTVVISQSGETADTLAAQREAKHKGSKTLAICNVVGSMVTREAAG
                     TIYTHAGPEIGVASTKAFTCQLTALFILAMHLGQLRKILADDVSRCLVQQLLHVPAKL
                     EEVLGQDGLIEDLSKKLFRATNFLFLGRGIHYPIALEGALKLKEISYIHAEGYPAGEM
                     KHGPNALIDENLPAVVIATHDPKRPESLVQYEKTLSNIKEVKAREGIVIALANRKDQE
                     VRDAVDYMIELPPAEELLLPIIEIVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(2809..3054)
                     /gene="purS"
                     /locus_tag="VFA54_03895"
     CDS             complement(2809..3054)
                     /gene="purS"
                     /locus_tag="VFA54_03895"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HZS49978.1"
                     /translation="MKAIVQVALKKTVLDPQGQTIRRSLASLGYSGIRDVRQGKFFEI
                     DIENGANREEARREIEKMAREVLTNPVIEEFTYRLED"
     gene            3297..3704
                     /locus_tag="VFA54_03900"
     CDS             3297..3704
                     /locus_tag="VFA54_03900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS49979.1"
                     /translation="MIEYRIGNQIDLDQVIELYRASTLGERRPIGDRPRMAAMLTNAN
                     LVVTAWDGDLLVGIARSLTDFVYATYLSDLAVRVSHQRRGIGRELIRVTQSAGGPAKI
                     VLLAAPKAVTYYARVGLTPHPSAWTLAPDQKLR"
     gene            complement(3788..5161)
                     /gene="mnmE"
                     /locus_tag="VFA54_03905"
     CDS             complement(3788..5161)
                     /gene="mnmE"
                     /locus_tag="VFA54_03905"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZS49980.1"
                     /translation="MPSWNPEDTIVAISTPPGRGGLGVVRVSGPDARTIGERLVKFRT
                     EGRTWDPWRAAFGDLLDDQGRTIDQVVVTYFARPHSYTAEDLVEISCHGAPVVLRHTV
                     ERAIREGARLAEPGEFTMRAFLNGRIDLVQAEAVRDLIESTTLYQARVAAQQAEGAVS
                     RRLSPIKAQLLELIALLEAGIDFAEDDINIAPDAELLRRLEAISSALDGLASSFQFGK
                     VVTAGLTLAILGRPNVGKSSLFNRLLEQERAIVTEIPGTTRDLVSEMASIRGIPMKLL
                     DTAGIRPTQDRVESFGVQKSYEALVDSDLTLLVLDRSQPLGSIDYDLLTRTQNLKKRI
                     VVCNKCDLPAAAELGGIPDAAVHVSAATGEGIDRLRDAIYEAAVPRLSGGEETGFITS
                     LRHEQLLREALDALGRARAAIGARTPHEMLLLDLYTALRAVDAITGATTVEDILNQIF
                     STFCIGK"
     gene            complement(5353..5877)
                     /locus_tag="VFA54_03910"
     CDS             complement(5353..5877)
                     /locus_tag="VFA54_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HZS49981.1"
                     /translation="MVSLNWTAEEVGPKVEKFLRPLLKAAGWALDFKIAEGSAIDPAF
                     EKPDVIVSFEGRDVDLLLANKGELLLALEHVTLEALRVRPEEHSRLLFDADDYRMLRV
                     EELRLSAETAAEKVKRTGIPFKFSPMNSRERRIIHLALRNDPAVRTESEGVAPHRQVV
                     IFPANHQTSKKSGR"
     gene            complement(5899..7605)
                     /gene="yidC"
                     /locus_tag="VFA54_03915"
     CDS             complement(5899..7605)
                     /gene="yidC"
                     /locus_tag="VFA54_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZS49982.1"
                     /translation="MNEEQEQKSIQIRALLAFVLSAAILFLYSRYVVKSQPPKPPAVA
                     TAPASPPAPAKGVVQAAEPKPPAGSPQRPVESAAQEQTFLVESGNYLVSLSNRGAVVT
                     SWTLKNYQDETGRPLELVNPAAAPEAGFPFSYAPQSELNQDLFQVESSAGPPGSTQQV
                     RLHAPVTLTFRWSDGRITARKIFQFSDDSYVVQVQSDLIRDGAPWPHLLTWRGGFGDF
                     AVEDSARNVKTFYFDAAQNKVIRLDAKAAAKGPVDNVGNYLYAGVEDHYFAAAFLTQN
                     RGNPLTLQTTTVEAPTSHESKKQPFASAAVGGAGDNKFLVYVGPKSLATLRAIQPQLA
                     QLVDFGFFSFIAYPLFLMLTWTHHWLPNFGWAIVVVTVIINFILFPLKLKSMQSMKKM
                     QRIQPLIKHINDKYKNLSMRDPKRQEQNKEVMELYQKYGVNPLGGCLPMLLQVPFFFA
                     FYAVLNWSIEMRHMSWLWVKDLSAWDHLYLLPIAMVATQFFYQKMTPSTSADPAQQKM
                     MQFMPLIMGFFFYRLPSGLVLYYLTSNLVGILQQWFINRLPEPELETGKLKHRPKLKA
                     NR"
     gene            complement(7602..7841)
                     /gene="yidD"
                     /locus_tag="VFA54_03920"
     CDS             complement(7602..7841)
                     /gene="yidD"
                     /locus_tag="VFA54_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZS49983.1"
                     /translation="MKTILLSLLTAYKRAISPLLPPSCRFYPSCSDYAREAVQLHGVA
                     RGACLAARRLLRCQPFCRGGLDPVPARAGRPSRFG"
     gene            complement(8353..8502)
                     /gene="rpmH"
                     /locus_tag="VFA54_03925"
     CDS             complement(8353..8502)
                     /gene="rpmH"
                     /locus_tag="VFA54_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZS49984.1"
                     /translation="MPKRTFQPNRRHRAKTHGFRARMKSPGGRTVLARRRAKGRHSLT
                     VSSEK"
     gene            complement(8691..10061)
                     /locus_tag="VFA54_03930"
     CDS             complement(8691..10061)
                     /locus_tag="VFA54_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS49985.1"
                     /translation="MLKVALLAALALCVAQATPLHGRVTAQDGKAVAGVKVAASPFSR
                     NLDTVFAVSNASGEFSFDLPSGDYVVYVDAPGYEMAIDAARPGGAPVVFTLRPAAPPL
                     LPETFLTSARSTEKALLDEQYQQIERYLDRLIAESEAKRPTKWNRDFSSLERYLASVE
                     PHRRKLIELLGGFPAVKTSLEARSELLGENESYRTERVWLEALPGVEAYGILLTPKRA
                     GKHPALICQHGMGGSPAMTAGVSERDDYMRRFGAQAAERGYVVFAPYIISDGAQRNRL
                     HRKAISVGQTLQAIEQWKMMRVTDFLASLPEVDAARIGIYGISWGGRTALYQAAIDPR
                     LAAAAISGYFNQTVKKQFEPSAYSTPYIDTAEEYAFFPRFITEFSDADLASLVAPRPL
                     FIESGTLDTAAYFKDSFIEFTRAKEIYRRLGFEDRIQFGLFRGVHLIHGTQAFEFLDK
                     WLRPSA"
     gene            complement(10055..10813)
                     /locus_tag="VFA54_03935"
     CDS             complement(10055..10813)
                     /locus_tag="VFA54_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HZS49986.1"
                     /translation="MAERILSVMAHPDDAEFLCAGTLILLHNRGFEIHIASMTPGDCG
                     SRELPADQIAQVRIEEARAAAAVIDASYHCLDCRDLVVCYDAPTIRRTVELIRSVGPS
                     IVITHSPHDYMADHEFTSLVVRNACFGAPAPNFKTGVSPGAAPTRRLPHLYYAAPIEG
                     MDIFGQPLPASVIIDISDVIGLKSSMLACHASQREWLRAQHGIDEYIETMRRWSECTG
                     RRIGVTYGEAFRQHVGHAYPKDDLLAELLGGRRC"
     gene            10934..11749
                     /locus_tag="VFA54_03940"
     CDS             10934..11749
                     /locus_tag="VFA54_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49987.1"
                     /translation="MRLCLCLLAAALAAGARAEIRGVLKSAEIDSMLSHAAADVPIHE
                     RPHYSIWLIARSGKAGSLESHSNTDELLFVRQGSAVISLGSGAKTGGTKRLEAAAGDV
                     IKISAGAPHQVDPGSSRFEALALRILAASASVKPREGIRPPARQMPDLLRESEIDATF
                     ADFTSNQPIHSAPNFTMNYVIYTGYSGPWEAHAGCVDIYFLNIGSATAQIGGVIANAR
                     EDVPGEPRGDGVSGASIYTVGPGDLVVIPRNTAHHMDPGAGKLGYVLVKVWVD"
     gene            complement(11746..12738)
                     /locus_tag="VFA54_03945"
     CDS             complement(11746..12738)
                     /locus_tag="VFA54_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZS49988.1"
                     /translation="MATYKIVIPDDYPPVLAPSQAYRDLIARTPVEYYDSLPGSEQRL
                     IERIASAEVVINIRSSSKFTEDVFKSCPKLRLLSLWGTGTDNVDLPAAARYGVTVTNT
                     PGVSAIAMAEHALALMLAVARNIPRVDANTRLGQWPRGSAAQMHGKTLGIIGLGAIGR
                     QFARLGAGIGMKVLAWTMHPDPAVIAQLPVQMAERDEVIRQSDVVSLHLRLSPETKGF
                     FGKREIGLMKPTAFLINTARGQLVDEAALIEALRSRRIAGAGLDVFETEPLPRGHAIT
                     ALENVVITPHSGGVTPEALEAGLQLSVDNMWNFLAGRPTNVVAGPAAVTDARGR"
     gene            complement(12757..13854)
                     /locus_tag="VFA54_03950"
     CDS             complement(12757..13854)
                     /locus_tag="VFA54_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49989.1"
                     /translation="MPIDSCKYLLCAGLLFVPSSARADAPPSFAVSPASLTFTAGDPP
                     QTVTVNGTGAPNWSASVTPPAGGAWLIITEPSSGPVGGSFQVSIANGGLATGTYSGSI
                     AVSVPGIGVQNIDVTFIVPPPPPPTVLEGGVVNAASFRGSAGVAPGEIVTLFGRGLGP
                     DVGAAAPAISQTGFLPTSLAGVSVTVNGAPAPLFFVRQDQISLQMPYDLGAQNSAELQ
                     VQFNGMQSQPVVVPVVSAAPGIFTLHPTGGQAAALNQDSSPNSVFNPAKAGSVVQLFL
                     TGQGKLKATAVAGQPSPVSGPFPAPLQTVSASIGGIDARVTFAGMAPGLTGVLQVNVQ
                     VPDSFFPLENVPVIVTVGGAQSAGNVTIAVQ"
     gene            complement(13887..>14823)
                     /locus_tag="VFA54_03955"
     CDS             complement(13887..>14823)
                     /locus_tag="VFA54_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HZS49990.1"
                     /translation="RVGHFSDYEALTGCTVVLCEQGAAGGIAVFGSAASTRAVDAFAP
                     GHLVEKVHGVVFAGGSAFGLDAVAGVMRYLERRGIGFDTGVARVPIVGGAILYDLAAG
                     SSAVRPTAEMGEAAANAAGDGPVEEGSIGAGTGATVGKLFGLGQAMKGGVGTATVKAA
                     GGALVSALAVVNAFGDVLDPATGRPLAGARISAASRELAGMAECMKRGQVRKTFGGRV
                     QQSNTTLAVVATNARLTKIEASKLAAMAQAGFARTISPVHTTFDGDLIFALGTSEAEA
                     DLNVLGVAAAEAVAASILRGVKLARSLGGIPGLGD"
BASE COUNT         2880 a         4592 c         4481 g         2870 t
ORIGIN      
        1 gccaggcagg aaggcttcac cagcatgggc atctgggcca accggagaac gcccctggat
       61 cccgagacgg cgacggacgc cgcagtggcg cgcatccagg aaatgctccg gcgctcgggt
      121 ctccacattt cggtcattgg gtgctcggag agtcatatcg atccggaccc gcaggcccgc
      181 gcccgcgcta acagctattt cggcaaggtc atcgagctgg ccggaaggct tggcgcgcct
      241 cacgtcggca cgcagaccgg cggcatgccc ggaaggccgc tcgccgaaca ggtggatgag
      301 ctcgtgcggg tatacaccga aaaatatttc cctctctgcc agaagcacca tgtgcgcatc
      361 ctgtgggaac cttatgccgg ccgcccgaac atcgccaccg gaccggtagg atacgaggcg
      421 ctctttaaag ccttcggcga ttcgccgtat atcggcctgc aatacgaccc gtctcacctg
      481 gtctggcaga tgatggatcc gatccagtgc gcacgggact ttgtggacaa gatttacgat
      541 gttcacttaa aggacacgga gattcgctgg ccgattttga gaaagacggg catccatccc
      601 ctgaacaacg cccagtggtg gcgtttccgg ctgcccggct taggacagat cgactgggcg
      661 gcctttttca ccgtgctcat ggacgcgggc tacagcggcg cgatgaatat cgagcacgag
      721 gacccgcttt acggccaccc caaccagggc gtggatttca ctgacgacta caagacggga
      781 ttccgggtcg cgcatcaata cttgcgccag tacgtgccgg catgagactg cgggctattt
      841 tgatttttct ccccgatcgc gcccggtcac tcgaccgtga cgctcttggc cagatttcgc
      901 ggctgatcca cgtcgcagcc gcgccggacg gcgatatggt aggccaaaag ttgcagcggt
      961 acgatctcaa taataggtaa tagcagctct tcggcgggcg gcagctcgat catgtaatcc
     1021 accgcgtccc ggacttcctg atccttgcgg ttcgcgagtg cgatgacaat gccttcccgc
     1081 gcttttactt ctttgatatt cgaaagcgtc ttttcgtact gcaccaggct ttccggccgt
     1141 ttcgggtcat gcgtcgcgat caccaccgcg ggcaggtttt catcaatcag agcgttgggg
     1201 ccgtgcttca tctcgcccgc cgggtagcct tcggcgtgga tgtaggagat ctccttcagc
     1261 ttcagcgcgc cctcgagcgc aatcggatag tgaatgccgc ggccgaggaa gaggaaattg
     1321 gtggcgcgaa aaagtttttt cgagagatcc tcgatcaggc cgtcctgacc cagcacttcc
     1381 tcgagtttcg cgggaacgtg caacagttgc tgcaccaggc agcgggaaac gtcatcggcc
     1441 agaatcttgc gcaactggcc gaggtgcatc gccagtatga acagagccgt gagctggcag
     1501 gtgaaggctt tcgttgaggc gacgccgatc tcggggccgg cgtgggtgta aatggttccc
     1561 gccgcttcgc gggtgaccat cgagccaacc acgttgcaga tcgccagcgt cttcgatccc
     1621 ttatgcttgg cttcgcgctg tgcggccaga gtgtcggcgg tttctccgga ctgcgaaata
     1681 acgaccgtaa gagtctccgg ctgaatgatg ggatcgcggt accggaactc gctgccgtag
     1741 tcgacctcca ccggcagacg cgccagcctc tcgatcatga acttgccggc cagagcagcg
     1801 tgccaggacg tgccgcaggc cacgatcttc acctgccgaa agctggagaa ctcggacggc
     1861 gcgatatcca tttcatccag gtagatcttg cccgtctcgc ggctgacgcg cccgatcgtc
     1921 gtgtcccgga cggcccgcgg ctgttcaaag atttctttga gcatgaaatg cttgtagccg
     1981 cccttttcag ccatgacggg atcccacagg atgtgctgca cctgccggtt taccggccgc
     2041 ccttcgaaat ccgtgagccg cacgccttcg ggagtgagca cggccatatc gccgtccatc
     2101 aggaagaata tgtcccgggt gtaatgcagg atggcggaaa cgtcggaggc tacaaaatat
     2161 tcgttgtcgc caagcccgat caccgcgggc ggcccacatc gcgccgccac aatcttgttc
     2221 gggtcgcgcc gcgaaatgat ggccagcgcg aacacgccgc ccagttctcg caccgccgcc
     2281 cgagccgcat tttccaggtt ccccgctgct ttcccgttgt aatgcttctc gatcaggtgg
     2341 gcgattacct cggtatcggt ttcggtcgtg aaatgatggc cctccccaat gagctgtttc
     2401 ttgagagcga gaaagttttc gatgatgcca ttgtggacaa ctacaatgtc accggtacag
     2461 tcgcgatgcg gatgcgcgtt ttcttcagtg ggccggccat gcgtcgccca gcgcgtgtgc
     2521 ccgatgccgt agcttccgtc catcggattg atccggatgg cttcttccag attgcgaagc
     2581 ttgcccgagg agcggcgcac catgagcccg ttgtgctcgc cggctaccgc aattcccgcg
     2641 gagtcgtacc ctcgatactc gagtctccgc aggccatcca gaatcagggg gactacctta
     2701 cgggagccaa cgtatccaac aatgccgcac ataaaaagct cctcgcggcc gccgcccgcc
     2761 ggtcagcaaa ccgtagcgta accggcggaa agctggcgag gcgtagcctc agtcttccag
     2821 cctgtaagta aactcttcaa tcaccgggtt ggtcagcact tcgcgggcca ttttctcgat
     2881 ctcccggcgg gcttcctccc gattcgcgcc gttttcgatg tcaatctcaa aaaacttgcc
     2941 ctgccggacg tcgcgaattc ccgagtagcc cagcgacgcc agcgagcggc gaatcgtctg
     3001 gccttgcgga tccagcaccg ttttcttcag agccacctgc acgattgcct tcatggaagt
     3061 tttgtccggg cagcccgtgt ccggcatcca aggcagacgc caaaaccaca agccgcagcc
     3121 ttttatatcc attctattcc gtttttcggc gcataaacgt tgagaaattt tatgacagac
     3181 attgcaaaaa tctgcggtcc ggcgcggccg cggagccgag ccgctgggct tccgcagatt
     3241 ctgcatataa tgaaatcggt cattgcggca agcgccggac gtttgtctct tttctcatga
     3301 tcgagtatcg catcggcaat caaatagacc tggatcaggt catcgagttg tatcgggcat
     3361 cgacgctcgg cgaacgccgc cccatcggcg atcgcccccg gatggcggct atgctcacca
     3421 acgccaatct ggtggttacc gcctgggatg gcgacttgct cgtcggcatc gcgcgctccc
     3481 tgaccgattt cgtctatgcc acttatcttt ccgatctcgc cgtccgcgtc tcacaccagc
     3541 ggcgcggcat tggcagggag ttgatacggg taacgcaaag cgcgggcggt ccggcgaaaa
     3601 tcgttttgct ggccgctccg aaagcggtga cttactatgc tcgcgtcgga cttacgcccc
     3661 atccttccgc ctggacgctc gctcccgacc agaagctgcg ctgaaacact ctccggaatc
     3721 cctgattgag agaaactccg ctctgggggt cccgcgccga tctccttgat tttctccccg
     3781 tgcagattca tttcccgatg cagaatgtcg agaaaatttg attcaggata tcttccacgg
     3841 tcgtggctcc ggtaatcgca tcgacggctc gcagcgctgt gtaaaggtcg agcagcagca
     3901 tctcgtgagg cgtccgggcg ccaatggctg cgcgggcgcg tcccagcgcg tcgagagcct
     3961 cgcgcagaag ctgctcatgg cgaaggctcg tgatgaaccc tgtttcttcg cctccggaaa
     4021 ggcggggtac ggcggcttcg taaatggcgt cgcggagtcg atctatgccc tctccggtcg
     4081 ccgccgatac gtgaacggct gcatccggga ttcccccaag ctcagcagcc gccggcaggt
     4141 cgcatttatt gcagaccaca attcgttttt tcaaattttg cgttctcgtt agaaggtcat
     4201 aatcgatgct gccgagaggt tgcgaacggt cgagcacgag cagagtgaga tcggaatcca
     4261 ccagcgcctc gtagcttttc tgaacgccga acgactccac gcggtcctgg gtggggcgaa
     4321 tgcctgccgt gtccagcagc ttcatgggaa tcccgcggat ggaagccatc tccgaaacca
     4381 ggtcgcgcgt agttcccggg atttcagtta cgatggcccg ctcttgttcg agcagccggt
     4441 tgaaaaggct cgatttgccg acgttcggcc gtcccaggat ggccagcgtc aagcccgccg
     4501 tcaccacctt accgaactgg aagctgctgg cgagcccgtc cagtgccgag gaaatggctt
     4561 ccaggcggcg cagcagctcg gcgtccggcg caatgttaat atcatcttcc gcaaagtcaa
     4621 tgcccgcttc cagcagggcg atcaactcga gcaattgcgc cttgatgggc gacaaccggc
     4681 gcgagacggc cccttcggcc tgctgcgctg ccacgcgcgc ctggtataag gttgtggatt
     4741 caatcaggtc gcggacggct tccgcctgca ccagatcgat gcgtccgttc aaaaaggccc
     4801 gcatggtgaa ctcgcccggt tcggcaaggc gagcgccctc ccggatcgcg cgctccacgg
     4861 tgtgccgcag cactacgggc gccccgtggc aggaaatttc gacgagatct tctgccgtgt
     4921 aggagtgcgg ccgggcgaag tacgtcacca ccacttggtc aatggtccgc ccttggtcgt
     4981 cgagcaaatc gccgaacgca gcgcgccagg gatcccaggt ccggccctct gtacggaact
     5041 ttacaagcct ttccccgata gttcgggcat cgggtcctga aactcgcacc actcccagcc
     5101 cgccccggcc gggcggggtg ctgatggcca cgatcgtgtc ttccgggttc cagctaggca
     5161 agtagaaatc tcaggcgggc ggtaatccag tatccgatgg cgcggaggca taggggcgct
     5221 cggaccgatg cgcgaaagac catcttccgc tagacaggac gctttcagca aaatgcggga
     5281 aaattgccgg attcgacgga ccaagccgca tccatccctg attccgcacg gtcagtacag
     5341 cctccggtgg cctcagcgcc cgcttttttt tgaggtctgg tgattggccg ggaagatcac
     5401 cacttggcgg tgaggagcca cgccttccga ctcggttcgt actgcgggat cattgcgcag
     5461 cgccaggtgg atgatgcggc gctctcgcga gttcatcggg ctgaacttga aaggaatgcc
     5521 ggtcctcttg accttctcgg cggcggtctc cgcgctcagc cgcaactctt ccacccggag
     5581 catccggtag tcgtcggcgt cgaacagcag gcgggagtgc tcctcggggc gtacgcgcag
     5641 ggcttccaga gtcacgtgct cgagcgccag cagcaactcg cccttattgg cgagaagcaa
     5701 gtccacgtcc cggccttcga aagaaacaat cacatcgggc ttctcgaatg ccggatcaat
     5761 tgcggaacct tccgcaatct tgaaatcaag ggcccacccc gccgccttca gcaggggacg
     5821 cagaaacttc tccaccttgg gcccgacctc ttccgctgtc caattcaagc tgaccatgct
     5881 gagtctgacg cccggttgct aacggttcgc cttgagcttg ggtctgtgct tcagcttccc
     5941 agtttctaat tccggctccg gcagacggtt gataaaccac tgctgcaaga tgcccaccag
     6001 atttgaagtc aaatagtata gcaccaggcc gcttggcagg cggtagaaga agaaccccat
     6061 gatcagcggc atgaactgca tcatcttttg ctgcgccggg tcggcggagg tggaaggggt
     6121 catcttttga tagaagaact gcgtggccac catagcgatc ggaagcagat acaggtggtc
     6181 ccacgccgaa aggtctttga cccacagcca ggacatgtgg cgcatctcga tggaccagtt
     6241 cagcaccgca taaaaagcga agaaaaacgg cacttgcaac agcatcggca ggcagccgcc
     6301 cagcgggttc acgccatatt tctgatacag ctccatcacc tctttgttct gctcctggcg
     6361 cttggggtcc cgcatgctca ggttcttgta cttgtcgttg atgtgcttga tcagcggctg
     6421 gatgcgctgc attttcttca tcgactgcat gctcttgagt ttcagaggaa agaggatgaa
     6481 gttgatgatg acggtgacga ccacgatcgc ccagccgaaa ttcgggagcc agtgatgcgt
     6541 ccaggtgagc atcaggaaca aagggtacgc gataaagctg aagaagccga aatcgaccag
     6601 ttgcgccaac tggggctgga tcgcccgcag cgtcgccaga cttttcgggc cgacatatac
     6661 gaggaatttg ttatccccgg cgccgccgac ggccgcgcta gcgaacggct gcttcttgct
     6721 ttcgtgcgaa gttggggcct ccacggtggt ggtctgcaaa gtcagcgggt tgccgcgatt
     6781 ctgggttaag aacgcggcgg cgaaatagtg atcctccacg ccggcgtata aataattccc
     6841 gacattgtcc acggggcctt tggcggcggc cttcgcatcc aggcgaatga ccttattctg
     6901 cgcggcgtcg aagtaaaacg tctttacgtt gcgggccgag tcctcgacgg caaaatcgcc
     6961 gaacccgccg cgccaggtca aaagatgggg ccagggggcg ccgtcccgga tgagatcgct
     7021 ctgcacctgc accacgtagg agtcgtcaga gaactggaag attttccggg cggttatccg
     7081 gccgtcggac cagcggaacg tcagcgtcac cggcgcgtgg aggcgaacct gctgcgtgga
     7141 gccgggcggg cccgcggatg attccacttg gaaaagatcc tggttcagct ccgactgcgg
     7201 ggcgtagctg aaaggaaagc cggcctccgg cgccgccgcc gggttgacca attccagcgg
     7261 ccggccggtc tcgtcctgat aattcttgag cgtccagctt gtgaccaccg ctccccggtt
     7321 cgagagcgac accaaataat tgccgctttc gacaagaaac gtctgctctt gtgcggccga
     7381 ctctacgggg cgctgtgggg agccggcagg cggcttcggc tcggcggcct ggactacccc
     7441 tttcgccggg gccgggggcg aagcgggcgc ggtagcgacc gccgggggct tgggaggctg
     7501 cgatttcacg acgtagcgcg agtaaagaaa cagtatggcc gcagacagca caaaggccag
     7561 cagcgcgcga atctgtatcg atttttgctc ttgttcttcg ttcatccgaa cctggagggc
     7621 cggcccgctc gggcaggcac ggggtcaaga ccgccgcggc aaaaaggctg gcatcgcagc
     7681 agccgcctcg ctgccaggca ggctcctcgc gccaccccat ggagttgcac agcttcgcgg
     7741 gcgtagtccg agcacgaagg ataaaaacgg cagctcggag gcaggagcgg cgaaatcgcg
     7801 cgtttgtaag ccgttaagag tgagagtagg attgttttca cgacttgcgt ttcagcaccg
     7861 aaaaaaatcg cgcaacctcg gcccggatag ccccaaattc tgcgtccagc accgcccggc
     7921 gggggttaaa caccacattc caacccggcg cgaccgaagg caattccgct cgcacagcct
     7981 ctcgcaaccg acgccggatc cggttacggg caactgactt cccgatggat ctaggcgtcg
     8041 tgaagccaac ccgcgcaggc ctgccgttct ccgctcggtg gtagaaggcc gcgaacaagg
     8101 ggcccacaaa tcggttcccc tctttataga ttttacgata ttcggcgctt ttcagcagac
     8161 gcgccgattt cgggaagagg aagcggccgc tctgcacggt ttagcgcccc attgccggga
     8221 aactgccact tgtgaaacag gcccccgaaa aaccgcgtcc ggccaggggt gcagagatat
     8281 ctcggctgcc gggccgcgcc gcgaaagccg gcgcctcggg ccagcacaat gtccgaagtc
     8341 gaatcggcca cactattttt ctgagctgac ggtgagagag tgacggccct tcgcgcggcg
     8401 gcgtgccagg accgtgcggc cgcccggcga cttcatgcgg gcgcggaagc catgcgtctt
     8461 tgcccggtgg cggcggttgg gttggaacgt tctctttggc atcagatctc cagtctcaac
     8521 tgcaactatt tgattatatg acaggacgcc gggcctgtca ggcttcactg gggcaatcca
     8581 cagttattgc ttcctcgagg ccttgccgca agggagcgca atagatgcct cggacgcggc
     8641 gcgaacccgc gggcgtgtac cggcggttcg agagccgcgt gttgttgctc ttatgccgat
     8701 ggcctcagcc acttgtccag aaattcaaac gcctgtgtgc cgtgaatcag gtgcacgccg
     8761 cggaataagc cgaactggat gcggtcttca aaacccaggc gccggtagat ctctttggct
     8821 ctcgtgaact cgatgaagct gtctttgaaa taggccgcgg tgtcgagcgt tcccgattcg
     8881 ataaatagcg ggcgcggcgc tacgagcgaa gccagatcgg cgtcggaaaa ctccgtaatg
     8941 aagcggggga agaacgcgta ctcttccgcc gtgtcgatgt agggcgtcga atacgcagac
     9001 ggctcgaact gttttttcac ggtctggttg aaatagccag aaattgcggc ggcagccagg
     9061 cgcggatcaa tcgccgcttg atagagcgcc gtccggcctc cccaggagat gccatatatg
     9121 ccgatgcgcg ccgcatccac ctcgggcagc gacgccagaa aatcagtgac ccgcatcatc
     9181 ttccattgct cgatcgcctg gagcgtctga cccaccgaga tggctttccg gtgcagccgg
     9241 tttcgttgcg cgccgtcgct gataatatac ggcgcaaata cgacgtagcc gcgctcggct
     9301 gcctgtgcgc cgaagcgccg catgtaatca tcgcgctcgg aaaccccggc tgtcatggca
     9361 ggcgacccgc ccataccgtg ctggcagatc aaagcgggat gcttgccggc ccgtttcggc
     9421 gtgagcagta tcccgtaggc ttccacgccc ggcagcgcct ccagccaaac ccgttccgtt
     9481 cggtagctct cgttctcgcc gagcaactcg ctccgggcct caagagatgt cttcaccgcg
     9541 ggaaagccgc cgagcagctc gatcaacttc cggcggtgcg gctcgacgga ggcgaggtag
     9601 cgttcgagag aggagaaatc gcgattccac ttcgtgggcc gcttggcctc ggattcggca
     9661 atcagccggt ccaggtatct ctcaatttgc tggtattgct catccagcag cgccttttcc
     9721 gtggatcggg ccgaagtgag aaacgtttca ggaaggagcg gcggggcggc aggccgaagg
     9781 gtaaacacca ccggcgctcc gccaggccgc gctgcgtcga tagccatctc gtatccgggg
     9841 gcgtccacat agacgacata atcgccgcta ggcaggtcaa agctaaactc gccggacgca
     9901 ttggacaccg caaaaacagt atccaggttc cggctgaacg ggctggcggc gaccttcacc
     9961 cccgcgactg ctttgccgtc ctgcgctgtg acacggccgt gaagaggagt agcctgggct
    10021 acgcagaggg caagggctgc aagaagagcg actttcaaca tcttcggcct ccgagcaact
    10081 ccgccagcaa gtcgtctttt gggtacgcgt ggcccacgtg ttggcggaac gcctctccgt
    10141 acgttacgcc aatccgccgg ccggtgcatt ccgaccaccg ccgcatagtt tcgatgtatt
    10201 cgtcgatgcc gtgctgcgca cgcagccatt cccgctggct ggcatggcag gccagcatac
    10261 tcgatttaag accgatcacg tccgagatat cgataatcac cgaagcggga agcggctggc
    10321 cgaaaatgtc cataccttca attggcgctg cataataaag atgcggcaac cgccgcgtcg
    10381 gcgcagcgcc gggcgagacg ccggttttga agttcggcgc cggggccccg aagcaggcat
    10441 tgcggacaac caaactggtg aactcatgat ccgccatgta atcgtgcggc gaatgggtaa
    10501 tgacaattga aggaccaacc gaacggatca gttcaactgt ccgccggatg gttggcgcgt
    10561 cgtaacagac gaccaagtcc cggcaatcga ggcaatggta gctggcgtca atgaccgctg
    10621 cggcggctct ggcttcctca atccgcactt gcgcaatctg atcggcgggt agttcacggc
    10681 tgccgcagtc gccgggcgtc atcgatgcga tgtgaatctc gaagcctctg ttatgcagaa
    10741 gaatcagggt tccggcgcag agaaattcgg cgtcatccgg atgagccatg accgacagga
    10801 tccgctctgc catctcggaa aatagtacca ggatcgggag cgctcggcct cgataaatcc
    10861 gcggcggcgc aatctgcgcg ttgcttgccc tcccataccg gtttagtatg atggaagccc
    10921 ctgggaggct cctatgcgcc tctgtctatg cctgctggcg gccgctctag cagccggcgc
    10981 tcgagccgag atccgcggcg ttctcaaaag cgcggagatt gactcgatgt tgtcgcacgc
    11041 cgccgccgac gtcccgattc atgagcggcc gcattactcg atttggctca ttgcgcgctc
    11101 ggggaaggcg gggtctctcg aaagtcattc gaacacggac gaactcctct tcgttcggca
    11161 gggaagcgcc gtgatctcgc tcggatccgg ggcgaagacc ggcgggacca aacgcctgga
    11221 ggcggccgcg ggcgacgtca tcaagatctc ggcgggcgcg ccgcatcaag tcgatcccgg
    11281 cagttcccgt ttcgaggccc tggctttgcg tattttggcc gccagcgcaa gcgtcaagcc
    11341 gcgcgaggga atccgcccgc ctgcgcggca gatgcccgat ttattgagag aatccgaaat
    11401 cgacgctacc tttgccgact tcacatccaa tcagccgatt cactccgccc ccaattttac
    11461 gatgaactac gtgatctata cgggttacag cggcccatgg gaagcgcacg ccgggtgcgt
    11521 ggacatctat ttcttaaata tcggctcagc cacggctcaa atcggcggcg tgatcgcgaa
    11581 cgccagagag gacgtccccg gcgaaccgcg cggagatggc gtctccgggg cgagcatcta
    11641 cacggtaggg ccgggcgatc tcgtcgtgat tccccgcaac acggcgcacc acatggaccc
    11701 cggcgcgggc aagctgggct atgtgctggt aaaggtctgg gtcgattagc gcccgcgtgc
    11761 gtcggtaacc gcggccggtc cggcgaccac gttggtcggt cttcctgcca gaaagttcca
    11821 catgttgtcg accgagagtt gaagtccggc ctccaacgcc tcgggcgtta cgccgccaga
    11881 gtgcggcgtg atgaccacat tttccagcgc cgtgatggcg tggccccggg gcaatggctc
    11941 cgtttcgaaa acgtcgaggc cggcgccggc aatcctgcgg ctgcgaaggg cctcaattaa
    12001 cgccgcctca tccacaagct ggccgcgcgc agtgttgatg agaaaggccg tgggcttcat
    12061 cagcccgatc tcacgcttcc caaaaaagcc tttggtttcc ggtgaaagcc gcaggtgcag
    12121 gctgacgaca tcgctttgcc gaatcacctc atcccgctcc gccatctgca ccggcaattg
    12181 cgcgatgact gccgggtccg gatgcatggt ccaggcgagc accttcatcc cgatgcccgc
    12241 acccaaacgc gcaaactggc gtccgatggc tccgaggccg atgattccaa gcgttttgcc
    12301 gtgcatttgg gcggccgacc cccgcggcca ttggccgagg cgcgtgttgg cgtccacgcg
    12361 gggaatattg cgcgccaccg ccagcatgag agccagcgca tgctcggcca tcgcgatcgc
    12421 ggaaactccg ggcgtgttgg tgaccgtaac accgtagcgc gcggcggcgg gcagatctac
    12481 gttgtcggtg cccgtgcccc agagcgagag cagccgcagc ttgggacagc tcttgaaaac
    12541 atcctcggtg aatttggacg aggaccggat attgatcaca acctcggcgg aagcaattcg
    12601 ctcaatcagc cgttgctccg agccgggcaa agagtcgtag tattcgaccg gcgtccgggc
    12661 gatcaggtct ctataggcct ggctcggcgc gagcacaggc gggtagtcat caggaattac
    12721 aattttgtaa gtcgccatag atttgtatcg ctgtttttat tggactgcta tcgtaacgtt
    12781 cccagcgctt tgcgccccgc ctaccgttac gattacggga acattctcca ggggaaagaa
    12841 agaatcaggc acttgaacgt tgacctgaag cacgcctgtc agtcccggcg ccatccccgc
    12901 aaacgtcacc cgcgcgtcaa tgcccccgat ggaggcgctc acggtttgga gcggggcagg
    12961 gaaggggccc gaaaccgggg acggctgccc ggctaccgcc gtggccttca gcttaccctg
    13021 gcctgtcaag aataattgca ccacgctccc ggccttggcc gggttgaaca ccgagttcgg
    13081 cgaggaatcc tgattcaggg cggcggcctg cccgccggtt ggatgcagag tgaaaattcc
    13141 cggtgcggcg ctgaccacgg gcacgacaac cggctggctc tgcatgccat tgaattgcac
    13201 ctgcaactcc gcggaattct gggcgcccaa gtcgtaaggc atctgcaagc tgatttgatc
    13261 ctggcggaca aagaagagcg gggccggcgc gccattaaca gtgacgctga ctccggccag
    13321 ggaagtcggc aagaaaccgg tttgcgagat cgctggagcg gcggccccta catccggccc
    13381 cagtccccgg ccgaacagcg tcacgatctc gcccggagca acccccgcgc tgcctcgaaa
    13441 actggctgcg ttgacaactc cgccttcaag cacagtgggt gggggcggcg ggggaacaat
    13501 gaaagtcaca tcgatattct gaacaccgat tcccggaacg ctcaccgcga tgcttccgga
    13561 gtaagtgccc gtggcgagac cgccgttggc gatcgaaact tgaaagctcc ccccaacggg
    13621 gcccgaactg ggctccgtga tgatcagcca agcgccgccg gccggcgggg ttacgctcgc
    13681 cgaccaattt ggagctccgg tgccgttcac agtaacggtt tgaggagggt ctcctgctgt
    13741 aaaagtgaga ctggcggggc ttacggcgaa gctgggcggg gcgtccgcgc gggcgctcga
    13801 cgggacaaac aaaagcccgg cgcacagcag gtatttgcag gagtcgatcg gcataagagg
    13861 agattatacg gccatcgggc gcgaattcaa tcgcctaaac ccgggatgcc tcccaaagac
    13921 cgggccagct ttacgcctcg caggatcgat gcggctacgg cttctgcggc cgctactcct
    13981 agtacgttca gatcagcctc ggcttcgctc gtgccgaggg cgaagatcag gtcgccgtcg
    14041 aaggtggtat gtactggcga gatggtgcgg gcgaatcctg cctgggccat agcagcgagc
    14101 ttactggctt cgattttcgt cagccgggca ttggtggcga caacggccag cgtggtgtta
    14161 ctctgctgca cgcggccgcc gaaggtcttc cggacctgac cgcgcttcat acattcggcc
    14221 attcccgcca gttcccggct ggctgccgag atgcgcgccc cggctaaggg gcggccggtt
    14281 gcgggatcca gcacgtctcc gaacgcattc acgactgcca gcgcggaaac cagcgctccc
    14341 ccggcggctt tcaccgtggc cgtgccaacg ccgcctttca tcgcctgtcc aagtccgaac
    14401 agcttgccaa cggtggctcc cgtgcctgcc ccaatcgaac cttcttccac ggggccatcg
    14461 ccggcggcgt ttgcggctgc ttcccccatt tccgcggtgg ggcggacggc gctcgacccc
    14521 gcggccagat cgtacaaaat agcgcctccg acgatgggga cgcgggccac tcccgtatcg
    14581 aaaccaatcc cccgccgctc caaataccgc ataacgccgg cgaccgcgtc caagccgaaa
    14641 gcgctgccgc cggcgaaaac cactccgtgg accttttcaa ccaggtggcc gggcgcaaag
    14701 gcgtctacag cccgggtgct cgccgcagat ccgaaaacag cgattccgcc tgcggctccc
    14761 tgctcgcaga gaaccacagt gcacccggtg agcgcttcat aatcggaaaa atggcccact
    14821 cga
//
LOCUS       DASREK010000075        11710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1199720, whole genome shotgun sequence.
ACCESSION   DASREK010000075 DASREK010000000
VERSION     DASREK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11710
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199720"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VFA54_03960"
     CDS             <1..847
                     /locus_tag="VFA54_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49991.1"
                     /translation="VQAQHERRKYLDCSGGQKQLLKFAGLGRYGRARLQRARQLFEAG
                     FGSRVYGLKNGFLLAEFSPGTPLSADGAIGLGAARDSFLDYLSRYLSWVAQNCPSGRA
                     IPFDDLLRMIETNVSELLGPQWTGRVAAFERSRGLIAGGKSIELDGRMLPHEWLATPD
                     GWRKTDGVEHHDDHFFPGCQDIAWDVAGAQIEFSLGAGGRDGLTRRFLLFAPDAALPA
                     RLPFYRLAYLAYRAGYSAMQADSLKESPDGRRFRALSRRYTALLKREIRRSPRSPRAF
                     GGPFL"
     gene            complement(895..1503)
                     /locus_tag="VFA54_03965"
     CDS             complement(895..1503)
                     /locus_tag="VFA54_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HZS49992.1"
                     /translation="MGNIAAFGIYPTYASVESAVDALRAAGFRKTDISVLFPENAGSK
                     DLAHVKATKAPERAAAGAASGAVIGGALGWLVGVGALAIPGLGPLIAAGPIVSALAGV
                     GAVGTVGGLAGALIGLEIPEYEAKRYEGRIKRGGILLSVHCDDANWAKRAKRLLERTG
                     AEDISTAGEAKADYARSDKPLPRSFTGGDDHTSRTEAYKRRH"
     gene            1773..1982
                     /locus_tag="VFA54_03970"
     CDS             1773..1982
                     /locus_tag="VFA54_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49993.1"
                     /translation="MAAGSAKAGDAMYIEEKIVWHCGRDVRARLTYELRRGIRGYTEA
                     RLVGIEELAEESPEIDRAEELVLQQ"
     gene            complement(2128..3171)
                     /locus_tag="VFA54_03975"
     CDS             complement(2128..3171)
                     /locus_tag="VFA54_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZS49994.1"
                     /translation="MHTSSDGPFLRRSLLLLTLLAGFALIWYAADVFILAFLGILLAI
                     LLDALTQWTAARTKLARGWCFLLVVLAVAALVAIGIWQLGPRVILQANQLVGSLPRLL
                     GNVRQYLGKYEWGKVILRQMPAELGTNVIGKATALSSKTFEAISGVVVALIVGLYVGA
                     DPQFYIRGLLRLFPPDKANMAERVLAEVGYTLRWWILGQLVPMIVLGAATMAGLWLLG
                     VPLAFTLGLFTALMVFIPYLGSLIALLATVLVALTQSATLVLYSAVFFLGIHAAEAYL
                     LTPIVQKRAVFLPPALTILVQLLMGILLGFLGLMVATPLTAAALVLVKMLYLHEKPEH
                     HAESPPSAQRGAA"
     gene            complement(3817..5715)
                     /locus_tag="VFA54_03980"
     CDS             complement(3817..5715)
                     /locus_tag="VFA54_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZS49995.1"
                     /translation="MNLRSAVTSVVRRMAYQPIENYGIIGDLHTAALVGKNGSIDWLC
                     LPSFDSPSVFGAILDDEKGGRCQIAPKVGDVVYKQFYWPETNVLVTRFFSQDGVGEVT
                     DYMPVGEASRNRRELVRRISVTRGNMTFRLECQPAFNYARDRHRIHKSSEGVCFQSPA
                     LSLALCSTIALTPDEKGGVTAEFSLGEGQAAIFLLAQVAVGDSSVHVLSAEEEASLFT
                     ATVQYWRRWIARCTYRGRWREMVYRSALVLKLLTYQPTGAIVAAPTCSLPERIGGIRN
                     WDYRYTWIRDAAFTLYALMRIGFIEEAGSFMQWLEARCHELKPDGFLQIMYGIDGRHE
                     LREEALTHLEGYRGSRPVRAGNNAYNQLQLDICGELMDSVYLYNKYGSPISYELWKDL
                     CRLLCWVCDNWQQRDFGIWEVRGQPQQFVYSKVMCWVALDRALRLADKRSFPAERERW
                     LRSRDAIYEDILNNGWSEEMRAFTQYYGSKSLDASTLIMPLVFFLSPSDPRMLATLDA
                     INRPLKEGGLVSSGLVYRYHAKRDVDGLEGDEGTFNMCTFWLVEALTRAARTDRARLE
                     EAQLVFEKMLGFANHLGLYAEQTGACGEALGNFPQALTHLALISAAFNLDRALSNGME
                     WPGVTGPR"
     gene            6130..6690
                     /locus_tag="VFA54_03985"
     CDS             6130..6690
                     /locus_tag="VFA54_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZS49996.1"
                     /translation="MWGKNESPNSPAVNRPPAPAPARSPASTHEDHTPPALHTAPADR
                     TEVVRSGQSVIGKSLVLKATISGSEDLYIDGKLEGSVEMHDHNVTIGPNGNVSANVVA
                     RNITVHGALRGDLRASGKIEVCKTGSLLGDVTTAGISIEDGAYFKGSVDIIRPDQKKE
                     ADSKKDRPDPMKPEPPIKAPLKVGAS"
     gene            7348..8148
                     /locus_tag="VFA54_03990"
     CDS             7348..8148
                     /locus_tag="VFA54_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS49997.1"
                     /translation="MILFAAPVGQVNVETIIQRSTEANQADWKAAPDYSYFEEDKYED
                     GSSKTYEVRMILGSPYRELVAVNGAPLSPEDQEREKRKLNEAIQRRQQESPRERRQRV
                     GRYQRDRQRNRLLMQQLTKAFDFRLLGEEKLDQYEVYELEAKPRPGYQPPNREAQVLT
                     GMEGKLWIDKNTFQWVKVEATVIHPVSIAGFLARVEPGTRFELEKAPVADGIWLPKHF
                     AVNSHSRILFVFGHSTQADETYFGYHKGSEAAGNALQPKVGQARAIQP"
     gene            8470..9183
                     /locus_tag="VFA54_03995"
     CDS             8470..9183
                     /locus_tag="VFA54_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZS49998.1"
                     /translation="MRRARTCLLLVAAACLTPSFAANEKPSEPVERLNQAAKVFDEVM
                     NTPDNGIPKDLLDGASCVIIIPGLKKAGLVIGGQYGKGVLICRGKNGVGWTGPTTVRA
                     EGGSFGFQIGASEVDVVMLVMNQGVRDKLIQSGFTLGADAGVAAGPVGRNAAAATDVQ
                     MHAQILSWSRSRGVFAGAVLQGSTLRRDDDDNKAIYGKYVDPKAIVNGEVPPPPAAKL
                     LIATLNKYSPKLAQPHKPV"
     gene            complement(9490..9717)
                     /locus_tag="VFA54_04000"
     CDS             complement(9490..9717)
                     /locus_tag="VFA54_04000"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HZS49999.1"
                     /translation="MLVESKRRSLAKAISWRVIASITTAVITWGLTGAPTLAVKVGLL
                     DSATKLFGYFLHERLWARIKFGLAKPPEYEI"
     gene            10025..11041
                     /gene="argC"
                     /locus_tag="VFA54_04005"
     CDS             10025..11041
                     /gene="argC"
                     /locus_tag="VFA54_04005"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZS50000.1"
                     /translation="MQPPSKIRAGIIGHTGYSGGELVKILQRHPRVEPVLLEHRRNAG
                     ERGSADGLIPAPIPWQAEASADGGISVLFLATPPEVSLELAPPALERGLPVIDLSGAF
                     RLRTPENYRRWYGAEHPRPDLLAEAAYGLPEFCRERIRGARLVANPGCYPTAANLALK
                     PLIEADVIDRTAGVICDAKSGVSGAGKKPTAKTHFCEVAENFSAYSILEHRHVPEVLL
                     TSGLAQSEFSFTAQLLPLHRGILETIYFRSKEPLDSQSIAAIYQGRYRNEPFVRIYSD
                     GAVPDLHAVAHSNFCDLGIRSDPASRRVVVISAIDNLVKGAAGQAVQNMNLLLGFDEV
                     DGLQ"
     gene            11038..>11710
                     /gene="argB"
                     /locus_tag="VFA54_04010"
     CDS             11038..>11710
                     /gene="argB"
                     /locus_tag="VFA54_04010"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZS50001.1"
                     /translation="MKLLIKLGGTLLDSGESRSSIARQLSDLAQDHRVAVVHGGGKRL
                     SRYLAAQGISSTFIRGFRVTTPETLDAVIKVLAGEVNLQLVAALQQAGVRAVGLTGVD
                     GGLVEAAPLSEELGAVGAIQRARAELLDLLTAQGYLPVVACLAGGARGEIYNVNADQM
                     AVACAIHFRAERLIFLTDVEGVLDAERKLIPELTADQADELIHRGVASGGMEAKLRAC
                     CSAIRQ"
BASE COUNT         2335 a         3506 c         3669 g         2200 t
ORIGIN      
        1 cgtccaggcc cagcacgagc ggcgcaagta tctggactgt agcgggggcc aaaagcaact
       61 gctcaagttc gccggcctgg ggcgctatgg cagagcacgc ctgcaacggg cgcggcaatt
      121 gttcgaagcc ggattcggct cgcgcgtata cggtctcaaa aatggattcc tgctggccga
      181 attttcccca ggaaccccgc tttctgcgga cggcgcgatc gggctcggcg ctgcccggga
      241 ttctttcctg gactatcttt cgcgttatct ctcctgggtc gctcagaact gcccctcagg
      301 ccgggcgatt ccctttgatg acctgctccg gatgattgag acgaatgttt ccgagcttct
      361 cggaccgcag tggacggggc gcgtggccgc tttcgagcgg tcccgcggcc tgatagccgg
      421 cggcaaaagc attgagctcg acggccgcat gctgccgcac gagtggcttg cgacgcctga
      481 cggctggcga aaaacggacg gcgtggagca ccatgacgac cattttttcc cgggctgcca
      541 ggacatcgcg tgggatgtcg cgggggcgca aatcgaattc agcctgggcg ccggcgggcg
      601 agacggttta actcgaaggt tcctgctgtt tgccccggat gccgccttgc cagcgcgcct
      661 gccgttttac cgcctggcct atttggccta ccgggcaggc tattcagcca tgcaggcgga
      721 ttcactgaag gaaagtccgg acgggcgcag atttcgcgct ctgagccgcc gttacaccgc
      781 tcttctcaag cgggaaatca ggcgttcccc tcgatccccg cgggcattcg gcggaccgtt
      841 tttgtgacgg cccggttgaa ggcgccgctt gtgcgtcccg ggcgcagctg ttccctaatg
      901 ccgtctcttg tacgcttccg tgcgggacgt gtgatcgtcg ccgccggtga agctgcgcgg
      961 aaggggcttg tcgctgcggg cgtagtcggc tttggcctct cccgccgtag atatgtcctc
     1021 ggcgccggta cgctcgagca gccgtttggc gcgcttcgcc cagtttgcat catcgcaatg
     1081 gaccgacagc aggatgccgc ctctcttgat gcgtccctca tagcgcttcg cttcgtactc
     1141 ggggatttcg agcccgatca gcgcgccggc caagcccccc acagtcccga ccgcgcccac
     1201 accggccaga gccgatacga tcggtcccgc cgcaatcagc ggcccgagcc cagggatggc
     1261 aagggcgccc acacccacca gccagcccag cgcaccgccg atgaccgcgc cggatgcggc
     1321 gccagcggcg gcccgctcgg gagctttcgt ggcctttaca tgggccaggt ccttggagcc
     1381 tgcattctcc gggaacagca ccgatatatc cgtcttgcgg aaacctgccg ctctcagggc
     1441 atcgactgcg ctttctacgc tcgcgtaagt aggataaatt ccgaaggctg cgatgttccc
     1501 cattgactcc tcctctggcc tttcttacga cgcattttga tcgccgaagt gaaacctccg
     1561 gattataaag gcgcgccctc cagccgcttg tcctgcgaaa tacagaagcg gaaaaatctt
     1621 acaaaccgca aaagaaatta catgatcggg agcggcggat ctgccggcga agctcatgcg
     1681 cccccatgct ttcgtttgga cggcttctgg ccggcggcac gccggttgat tctgcgtttt
     1741 tgtgctgtgt ctaacagtgg taccatgcat gcgtggctgc gggtagtgcg aaagcgggtg
     1801 atgccatgta tatcgaggag aagatcgtct ggcactgcgg acgcgacgtc agggcacgcc
     1861 tcacgtacga gctccggcgg ggaattcgag gctacaccga ggcacggctc gtggggattg
     1921 aggaacttgc agaagaatcg ccggaaatcg atcgcgccga ggagcttgtt ttgcagcaat
     1981 gagtcgtctt gccaccgcct cgcaccttag aaggcggtcg aagacctttc gtttttcgta
     2041 tatacaatcc aacaaaacac actcggcaga ctgatccgag gcggttccgg ccccggcgcc
     2101 gtgcttcccc ctgagcgggc gagcgtttca ggcagcgccg cgctgggcgg acggaggaga
     2161 ctctgcatga tgttccggct tttcgtgcag gtatagcatc ttgaccagca ccagcgcagc
     2221 ggcggtcagg ggagtggcca ccatcagtcc cagaaaaccc agcaaaatac ccatgagcag
     2281 ttggaccaag atggtaagcg ccgggggaag gaacacggcc cgcttttgga caatgggtgt
     2341 caggaggtag gcttcggcgg cgtgaatgcc tagaaaaaaa acggccgagt aaagcaccag
     2401 ggtggcgctt tgcgtgagcg cgaccaagac ggtcgccaac aacgcgatga gtgagcccag
     2461 gtacggtatg aagaccatca gggcggtgaa gagtccgagg gtaaacgcca ggggcacgcc
     2521 gagcagccat aagccggcca tggtcgcggc gcccagcacg atcattggta caagctgtcc
     2581 gagaatccac cagcgcagcg tgtagcccac ctctgcgaga acgcgttcgg ccatattggc
     2641 tttatcggga ggaaacagcc gcagcagacc gcgaatataa aattgcgggt cggcgccgac
     2701 ataaaggccg acgatcaaag ccaccacaac gcccgagatc gcctcgaatg ttttcgagga
     2761 gagcgccgtg gctttgccaa tcacgttggt tccgagctcg gcgggcatct ggcgcagaat
     2821 gaccttcccc cactcgtatt tccccaggta ttggcggacg ttgcccagca gccgaggcaa
     2881 gctgcccacc agttgattgg cttgcagaat cactcgaggt ccaagctgcc agatcccgat
     2941 cgctaccaag gcggcgacgg ccagcacgac gagtagaaaa caccacccgc gcgcaagctt
     3001 ggtgcgagcc gccgtccact gggtgagcgc gtcgagaaga atcgccagca agattccaag
     3061 aaatgccagt atgaaaacgt cggccgcata ccagatcaga gcaaagccag ccagcagggt
     3121 cagcaacagc agcgagcggc gcaggaacgg gccgtcgctg gaagtatgca aaatcgggat
     3181 gggatccacg tcgtgaagtc gaagggaatc tcgttctacg ctccattatg actgtttcct
     3241 ccgggccgca cagttgctgc cgagccgctc gcgcgtgaaa ggacacggtg cagcgccggg
     3301 atgcgtgtga acggatgcgg ctgtagaccg ggaccgtgcg ccgggcaggc ctctgatgtt
     3361 ttggcagtcg gtctggcacg tcgcttgctg acggagacga gggagagccg ccgggcgccg
     3421 gctgcgctga attctgaggt cactcggagc gagtgggtct cgcgcctagc tccgaggtcg
     3481 ttgctgccgg gccgccgcgg cgctccctat cgcgctgctc gtataggatg accatgacta
     3541 tcagaggtga aatctactgt gacagttgtt ttctgcatat aggcttgcag aaacacacaa
     3601 aactgcaaat gcgcggccga gagcttcatt tccacaaccg cgctccgggt gattgctggg
     3661 agaaacacaa gtccggctcg cggagaccgg ccttgctgga aactttcgag cgtcaagccg
     3721 ccgcatgatg ggagaggata gaatttcgcg agcgaagaag cggccgggta tgccgcgcgc
     3781 cagcccaaaa gacgattgtt cgccgccgcg cctggctcag cgcggcccgg ttacgcccgg
     3841 ccattccatt ccattgctga gagcgcgatc cagattgaaa gccgcgctga tgagagccaa
     3901 atgggtcagc gcttgaggaa agttgcctaa agcttccccg caggcgccgg tttgttcggc
     3961 atacaaaccc aggtggttcg caaagcccag cattttttcg aagacgagct gggcctcttc
     4021 caggcgtgcc cgatcggttc gcgcggcccg ggtcagagct tccaccagcc agaacgtaca
     4081 catattgaaa gtcccctcat ccccttccag gccgtccacg tcccgtttcg catggtagcg
     4141 gtagaccaag ccgctcgaaa ccaacccacc ttctttcagc ggccggttga tggcgtcgag
     4201 ggtcgccagc atccgggggt cgctggggga caggaagaac accaaaggca tgatcagagt
     4261 ggaggcgtcc agcgatttgc ttccgtagta ttgggtgaag gcgcgcatct cctcgctcca
     4321 gccgttgttg aggatgtctt cgtagattgc atcgcggctg cgcagccagc gctcgcgctc
     4381 ggcgggaaaa gaccgtttgt cagccagccg caaagcccgg tcgagggcga cccagcacat
     4441 gaccttcgag tagacgaact gctggggctg gccgcgcacc tcccagatgc cgaaatccct
     4501 ttgctgccag ttgtcgcaca cccagcacag caagcggcat agatctttcc aaagctcgta
     4561 ggaaataggg ctgccgtact tgttgtagag atacacggag tccatcagct caccgcaaat
     4621 gtccagctgc aattggttgt aggcgttatt tccggcgcgc accgggcgcg atccccggta
     4681 gccttcgagg tgggtaagcg cttcttcccg cagctcatga cggccatcaa tgccgtacat
     4741 gatttgcaga aagccgtccg gcttcagctc gtggcagcgg gcctcaagcc attgcatgaa
     4801 gctgcccgcc tcctcaatga acccaatgcg catgagagca tacagggtga aagcggcgtc
     4861 gcggatccac gtatagcgat agtcccagtt tcggatcccg ccaatgcgct ccggcaagct
     4921 gcaagtcggc gcggcgacga tggctccggt gggctgatac gtaagaagct tgaggaccag
     4981 ggccgagcgg taaaccatct cccgccagcg tccccggtat gtgcacctgg cgatccagcg
     5041 gcgccaatac tggactgtgg ccgtgaacag ggaggcttct tcctccgcgg aaaggacatg
     5101 gacagagctg tctcccaccg cgacctgcgc cagcagaaaa atggccgctt ggccttcgcc
     5161 cagcgaaaat tcggccgtca cgccgccttt ctcatccgga gtgagagcaa tcgtcgagca
     5221 aagcgccaga ctgagcgccg gcgactgaaa gcagacgccc tcggagcttt tgtgtatgcg
     5281 atgccggtcc cgggcataat tgaaggccgg ctggcactcc aggcggaatg tcatattgcc
     5341 gcgtgtcacg ctgatgcggc gcaccagttc ccggcgattc cggctggcct cgcccacggg
     5401 catgtagtcg gtaacttcgc cgactccatc ctgcgaaaaa aagcgggtga cgaggacgtt
     5461 ggtctccggc cagtaaaact gcttgtaaac cacgtctcca acttttggcg cgatttggca
     5521 gcgtcctcct ttctcgtcgt ccaagatggc gccgaatacg ctgggggaat cgaagctcgg
     5581 aaggcagagc cagtcgatgg atccgttttt acccaccagg gcggcagtgt gcaggtcgcc
     5641 tatgatgccg tagttctcga tgggctgata agccatgcgc ctcactaccg aagtgaccgc
     5701 agaccgcaag ttcaaggcta acccctgacc gcggaacggc gccgcaccgc ccggggatcc
     5761 ggcacgccgg cgcgcgggct caccggggaa cgcgcagcgg cgcgaaggat tcggccgcgc
     5821 cacccttttg tgcgacgcga ggtctattat ctccgaaagg aaaaatttcc ggcgagtggt
     5881 gagtttatta cactcgcagg gtcgatcctg cgccacagct ttggccccgc cgaagtgttt
     5941 ccaagaggtt gcgaataccg gtaattggca gtgcgattgc tcgcatggcc gcaatgtcag
     6001 gtatcttcgc cccgtgcgga attcgtgttc caagccaact tacagtctgc gcattctctt
     6061 cgcggatctg ggtgcgggaa accgggcaga gtggttattt ttgagatctg ggaaagagga
     6121 ggggcataga tgtggggaaa gaatgagagc ccgaattcac cagccgtaaa cagaccgccg
     6181 gcgcctgcgc cggcacgatc tcctgcgagc acccacgagg accacacacc gccggcgctg
     6241 catactgcac cggctgaccg gaccgaagta gtacggtctg gacagtctgt catcgggaag
     6301 tctctggttt tgaaagccac catctcggga agtgaagatc tctatatcga cggaaagctg
     6361 gaaggctcgg tcgagatgca tgaccacaat gtgacgatcg gtcccaacgg caatgtgagc
     6421 gccaatgtag tggcccgcaa cattactgtt cacggcgcct tgcggggcga tctccgggca
     6481 agcgggaaaa tagaagtttg caaaaccgga tcgctcctgg gcgatgtgac gacagcaggg
     6541 atcagcatcg aagacggagc gtatttcaaa ggcagcgtcg acatcatccg gcccgaccag
     6601 aagaaggagg cggattccaa gaaagaccgg ccggacccga tgaaaccgga gcccccgata
     6661 aaagcccccc tgaaagtcgg cgcatcctga ctttgtccgc tgctgcaagg cggccgccgg
     6721 agattccagc cgcccgtgtt gcgtcagatt ttcgaagacg gaagcggccc ccggcccact
     6781 tcgccgttct gcctcccggc atcgcgaagg gatccaaatc gcgggaatgg aagccgcttt
     6841 tgcgggagaa gttctcgggg ccgctctttc taccggcttg ccgcggccta ccggcttgcc
     6901 gcggcgcagc ccggcggccc aggccggtgc ggcggcgcca gctttcgcgc ggctttctct
     6961 caaccctcat cagtactatc gacgaccttc cgtcggcagc gattttgtaa cgatggagaa
     7021 ggtactcgcg tccccaggga atgcaggcga gaggtaaggt ggcaagtggg gggcgccccc
     7081 tgggcgcact ctctttgtgg aaaatttccc ggccccgcaa gtcgcgcctg gaccgggcag
     7141 ctccgccgag cggggagagt tccattcccc taaagaagcg accgagggcg catgattttc
     7201 ggcaaggccc agaggcataa attatcatcg cggctgcaag cgcgctttca tagcttcggc
     7261 cagcagaggc caacgggtct tcgagcctgg gctgccccga tctcccgagg ccattggccg
     7321 gttgccggca ttggaaccct tttcgcaatg atcctgttcg ccgctcccgt cggccaagtc
     7381 aacgtggaga ccattattca acgctccacg gaagccaacc aggccgattg gaaggctgcg
     7441 ccggattatt cctacttcga ggaggataaa tacgaagacg gaagcagcaa aacctatgaa
     7501 gtgcgaatga ttctgggatc cccctaccgc gaactggtgg cagtgaatgg cgcgccgctc
     7561 tcgcccgaag atcaagagcg cgaaaagaga aaactgaacg aagccatcca gcggcggcag
     7621 caggagtcgc cgcgcgaacg ccgccagcga gtgggaaggt accagagaga tcggcagcga
     7681 aaccggctcc tgatgcaaca actgacgaag gccttcgact ttcgcctttt gggcgaggaa
     7741 aagctggacc agtatgaagt ctatgaactg gaagcaaaac cgcgacccgg ttatcagccg
     7801 cccaaccggg aggcccaggt cctgaccggc atggagggca agctctggat cgacaagaac
     7861 acattccagt gggtgaaggt cgaggctaca gtgatccatc ccgtctccat cgcaggtttt
     7921 ctagcgcgcg tcgagccagg cactcgtttc gaactggaga aggcgcctgt ggcggacggc
     7981 atctggctcc cgaagcattt tgccgtcaac tcccattcac gaatcctctt cgtgtttggc
     8041 cactcgacgc aggccgatga aacgtacttt ggctaccata agggttcgga ggccgcggga
     8101 aacgcgttgc agcccaaagt gggccaagcc agggccatcc agccgtaatt gcgaaaactc
     8161 accccccgga ggacgccgac ggcatactgt ctgcggggtt gtgacagcgc atacggtgac
     8221 gcccgtcgca gcgccttttt tccgtcctgt tgcctactaa tacactctaa accgaaggac
     8281 gcagaccgtg catggcagtc ggcttgcagc gattagggcg gaggacgctg taagcgggag
     8341 atcgaaagtt cgcgcgccgg tctgcaagct gcgggcgcga gcatccgggc gcagaattct
     8401 gcgcttggga gccacgaaga cgacttctcc cgcgagccca agcgcgccca ttgcatcagg
     8461 agattgaaaa tgagacgagc acgaacctgt ttattgctgg tcgcggctgc gtgcctgacg
     8521 cccagttttg cggccaacga gaagcccagt gagccggtcg agcggctcaa tcaagcggcg
     8581 aaagtgttcg atgaagtgat gaacacgccg gataatggca tcccgaaaga cctgctggat
     8641 ggagcatcct gcgtgatcat cattccgggc ttgaagaagg ctggattggt catcgggggc
     8701 caatacggga aaggtgtgtt gatttgccgc ggcaaaaatg gggtgggctg gaccgggccc
     8761 actacggtca gagcggaggg cggcagcttt ggtttccaaa tcggcgcatc ggaagtggat
     8821 gtcgtgatgc tggtgatgaa ccaaggcgtg cgcgacaaac tgatccagag cggattcacc
     8881 ctcggtgcgg atgccggagt ggcggctggg ccggtgggcc gcaatgcggc tgccgccacc
     8941 gacgtgcaga tgcatgcgca gattctgtcg tggtcccgtt cgcgcggagt tttcgctggc
     9001 gccgtgctcc agggctccac gctgcggcgc gatgacgacg acaacaaggc gatctacggc
     9061 aagtacgtcg accccaaggc gatcgtgaac ggagaggtgc ctccgccgcc ggcagcgaaa
     9121 ctgctgatag ctactctcaa caagtactcg ccgaagctgg cacagccgca caagccggtc
     9181 tgaggagctc tgaccagcag aaagagcccc gagattgcct cgccgctatt tcccgtcgcc
     9241 agtatcttgt ggtgaaaaac ggtcggctcc ggactcggct tcccggcgcc gccgtttcgg
     9301 tccccgaggc aagccccggg catctttgct cttcgccgga tctacctcac cttgagccgg
     9361 tcgagtcgcc ggctcaaccg ggggaaaagt tcccgccggc agcgatacag gcatcatggt
     9421 agttggcctc ctggcccttt ttttcggcgt gaagaggcga gacctttaca tgcccatatt
     9481 tgctcttact tagatttcgt actcgggtgg cttggccaag ccgaatttga tgcgggccca
     9541 gagccgctcg tgcagaaaat atccaaacag tttggtagcg ctgtccaaca ggccgacctt
     9601 gacggctagc gtaggggcgc cggtcaggcc ccaagtaatg acagccgtgg tgatagaggc
     9661 gatcacccgc cagctaatgg ctttagccag gcttctgcgt ttgctctcga ccagcatcca
     9721 atatcccaat ctctatcggg aatggaatca ttatcgcaga tgacagccga tttgaggcgc
     9781 gcccatgccc gtgaaagcgc ggtcaagcag agacatccgc cgtgccctat gatgtaagtt
     9841 cacgccagcg ggccggtttc gttcgctggc aggagccaaa cccgacttgg ccttccggcg
     9901 gttcgcggga acgccggcgt ttcaccggcg tgcaaggcgg ccctttgggc cgccgtcccg
     9961 gcagaggcgc cgctggggcg ggaaggcgtg tggtatattt atacatcgaa ctgaatattt
    10021 aatcatgcag ccgccatcga aaatccgcgc ggggatcatc gggcacaccg gctacagcgg
    10081 cggggaactg gtgaaaattc tgcagcgtca cccgcgcgtg gagccggtgc ttttggagca
    10141 ccgccggaac gccggcgagc ggggatccgc ggacggcttg atcccggcgc cgatcccctg
    10201 gcaagctgag gcctcagcgg acggcggaat ttccgttctt ttcctcgcta cgcctcctga
    10261 agtttctttg gagctcgcgc caccggcctt ggagcgaggc ctcccggtta tcgatttgag
    10321 cggcgcgttc cggctgcgca cgccagagaa ttaccgccgt tggtacggcg cagagcaccc
    10381 ccggccggat ttgctggcgg aagcggctta cggcttgccg gaattttgcc gcgagcgcat
    10441 ccgcggcgcg cggttggtgg caaacccggg ttgctatccc acggctgcca atctcgcgct
    10501 caaaccgctg atcgaggctg acgtgatcga ccgtacggct ggggtgatat gcgacgccaa
    10561 gtcgggcgtg agcggggcgg ggaagaagcc cacggcgaaa acgcacttct gcgaggtggc
    10621 ggagaatttt tccgcttact ccatcctgga acaccggcat gtcccggagg tgctgctcac
    10681 ctcggggctg gcgcagagcg aattcagctt taccgcccag cttttgcctc tgcaccgggg
    10741 cattttggag accatctatt tccgctccaa ggagccgctc gattcgcagt cgatcgcggc
    10801 catttaccaa gggcgttacc ggaacgagcc gtttgtgcgg atctacagcg atggtgctgt
    10861 tcccgacctg catgccgtgg cacactccaa tttttgcgat ctcgggatcc ggtctgatcc
    10921 ggcctcccgc cgcgtggtcg ttatttcggc cattgataat ctggtcaaag gcgccgcggg
    10981 ccaggcggtg caaaacatga acctgctgct cggttttgat gaggtggacg gcctccagtg
    11041 aaattgctga tcaagctggg cgggaccctt ttggattctg gagaaagccg gtcgagcatc
    11101 gcgcggcaat tgtcggatct cgcgcaagac caccgtgtgg cggttgtcca cgggggcggc
    11161 aaacggctca gccggtatct ggcagcccag ggcatttcca gcaccttcat ccggggattt
    11221 cgcgtgacta ccccggagac tctggatgcc gtcattaaag tgctggccgg cgaagtgaat
    11281 ctgcaactgg tggccgcgct ccagcaggcg ggcgtccgcg cggtggggtt gaccggcgtc
    11341 gacgggggcc tggtcgaagc cgcgcccctt tccgaggaac tgggcgcggt gggcgcgatc
    11401 caacgcgccc gggccgaact gctcgatctt ctcacggcgc agggctatct tcccgtggtg
    11461 gcctgtctgg ccggcggcgc gcgcggcgaa atttataacg tcaatgccga ccagatggcg
    11521 gtggcctgcg ctattcactt cagggcggag agactgattt tcctgaccga tgtcgaaggc
    11581 gtcctggatg ccgaacggaa gctcatcccg gagcttaccg ccgatcaagc cgatgaactg
    11641 atccaccgtg gggtggcttc cgggggtatg gaagccaagc tgcgagcttg ctgtagcgcg
    11701 atccggcaag
//
LOCUS       DASREK010000076        47480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1693554, whole genome shotgun sequence.
ACCESSION   DASREK010000076 DASREK010000000
VERSION     DASREK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 47480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47480
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693554"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFA54_04015"
     CDS             <1..348
                     /locus_tag="VFA54_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylmenaquinone methyltransferase"
                     /protein_id="HZS50002.1"
                     /translation="EPLGMPAYIRGVHPTAIGNVMLTGINVPIRIGGVTVMPGDVVFG
                     DNEGVTFIPPHLVQRVVERAEVTQLHDEWTKEKLRTGKYKSSEIYSTPRDPALRKEYE
                     EWLAREKAKRGIR"
     gene            596..1516
                     /locus_tag="VFA54_04020"
     CDS             596..1516
                     /locus_tag="VFA54_04020"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZS50003.1"
                     /translation="MRISAPQASEAASATLVGARRVERAIARLQALPAFYPPLQKALR
                     LLEDPDSRIDYIHQVICSDQAMASRILRLVNSAYFAVPSEVRTISLAITLAGRDRIAA
                     LLRRFLSEELLAMLSNRKPAAGHVRETSLVTAAAASCIAERLLRSDCEEILLAGLLHN
                     IGELVFLSQFRENYEETLRLAGHMALEEAERIVFGVESRVAGKWLLEAWNFPPPFPAV
                     AEHCADPWGVHFAAAPLAVIVIVHTARKLADAWVADARRGRAQQGAAAEAASAIAPRA
                     LAVLGVDRCFLADIYRDLGEQIQRRQSLWT"
     gene            1622..1891
                     /gene="rpsO"
                     /locus_tag="VFA54_04025"
     CDS             1622..1891
                     /gene="rpsO"
                     /locus_tag="VFA54_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZS50004.1"
                     /translation="MPLAKEAKQSIITKYHTHAKDTGSPEVQIALLSERIQQLTEHFK
                     AHKSDHGSRRGLLMLVSKRRRLLTYLKAYDAERYKALIQKLGIRR"
     gene            2103..4226
                     /gene="pnp"
                     /locus_tag="VFA54_04030"
     CDS             2103..4226
                     /gene="pnp"
                     /locus_tag="VFA54_04030"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZS50005.1"
                     /translation="MTQTTQVEVGGRTLTLETGKIAKQANGAVLVRSGDSAVLVTAVA
                     AQEGREGIDFFPLTVDYREYTYAAGRIPGGYIKREGRPSEKEILTSRLIDRPIRPLFP
                     EGFHCETQIIAMVLSADPEFDPSVLSIVGASAALAISDIPFLHVIGAVRVGLVDGELI
                     AVPGYAKLKESKLNIVVAAAEEGIVMVEAAASEVDEAKMIEAINFGHENCKKIVAAIR
                     ELAEKVGKPKRKVTPPALDQALYTEIETKFGASLRDALDTAKHPKQESHELVRELKNQ
                     VLAGFAEQPEKAAEAKKIFEALQEKIFREDVILRRRRPDARGFDQIRPISCEVAVLPR
                     THGSALFTRGETQALVTATLGTKEDAQRQELLEEAESFKRFMLHYNFPPFSVGEVGFL
                     RGPGRREIGHGALAERALAPMIPEEEDFPYTVRVVSDILESNGSSSMATVCGATLSLM
                     DAGVPIKAPVAGIAMGLVKEGDKYAVLTDIAGAEDHYGDMDFKVAGTRDGITALQMDI
                     KTPNVSTEILEKALAQARTARLLILDKMLQTIAEPRVKVSPYAPRIYTMNIATSKIRD
                     VIGPGGKVIRAIIEQTGVKIDVNDDGTIHIASSDEASANKAMQMIGDITAVAEVGKTY
                     LGKVVRLVDFGAFVEIFPGTDGLLHISEVAEHRIRDVRDELKEGDQILVKVLALEGNK
                     IKLSRKAVLREQREKKKQMETQAAH"
     gene            4758..7928
                     /locus_tag="VFA54_04035"
     CDS             4758..7928
                     /locus_tag="VFA54_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS50006.1"
                     /translation="MNISETFIRRPIATSLLMFAVALFGVLAYSALPVADLPTVDFPT
                     LSVFASLPGASPDTMSSAVATPLERQFTTIAGLDSMISVNSTGSTQITLQFDLSRTQD
                     SAAVDVETAIAEAMPLLPPGMPSPPSFFKFNPADAPVMFLTVSSPTLPIWVLDDYAEN
                     RIAQRLSMVSGVARVNVWGAQKFAVRVQVDPNKLAARNIALNDIAAALRSWNVNLPTG
                     TLYGPHQAFNVLANGQLSDAAAYRRVVVAYRNGAPVRLEQVANVIDNVEDNKQESWIY
                     DREGRRKAVNLVIFRQPGSNTIEVIEAIKKVLGEFQAQLPPSVQLSIRGDRSKNIRDA
                     FHDMQSTMILTLVLVIMVIFLFLRNTSATLIPSMALPFSIVGTFAVMYVLGYSLDNMS
                     MMALILCIGFVVDDAIVMLENIVRHLEHGERPLEAAFSASKEIGFTIVTMTVSLAAVF
                     IPVLFMGGILGRLFREFAVTICVAILISGLVSISLTPMLCSRFLRPPNQAGHGRLYRA
                     TERFFQGMLRSYDLSLRWVLRHRPVMITIFFIVLGSTAYLFVKVPKGFIPTQDLDQLN
                     VSTEAAEGTSFYQMAELQRKIADVVKADPNVDSILSMIGGGWSGNTGNLSVMLKPRKE
                     RRLNVMQIIEELRPKLATFPNIRAFLTPPSAIRIGGRMSKSQYQFTVQGPDTAELYAQ
                     AARLEREIARLPGLQDVTTDLQIKNPRMNITIDRDRAAALQLNVAEIEGELYNAYGPS
                     WATTMYLPTSQNRVLLELLPQYQGRPDSLPHLYFRSTSGNLVPLTSFARLTEDAGPLT
                     INHSGQLPSVTVSFNLKPGVALGEAVAEIKEVGERNLPATITTTFQGEAKAFQDSLRN
                     LGILLTVAIMVVYIVLGVLYESYVHPITILSGLPSAGFGALITLLLFKVDLNIYAFVG
                     LIMLIGIVKKNAIMQIDFALEAERKEGKTPAEAIYEGCLIRFRPIMMTTMAALLGALP
                     ISFGYGAGGEARRPLGLAVAGGLLFSQMITLYLTPVVYTYMASIVQRWNASRFGRKGR
                     AAAAQGIPALGGPVDSPLQPAAAKGD"
     gene            complement(8071..9735)
                     /locus_tag="VFA54_04040"
     CDS             complement(8071..9735)
                     /locus_tag="VFA54_04040"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HZS50007.1"
                     /translation="MKQISALDPGVLSLEEKVGQLFLLAFEGASAREAKPLLRDCFVG
                     SVYLSQDNLTGVDQAVQLLCDLQDTGAATPHALPILAACDQEGAWSVLNPYSTAGPGA
                     LALGAARSDLIEAMFAVIGRELRVLGISADLAPVADVNCNPRNPIIGSRSFGGDAKRV
                     AEAVEAAVRGLHRGGVVACAKHFPGHGDTAQDSHQGAGVVARDAQQIRNGDLIPFRAA
                     IAAGVDMIMTAHLLYPAFDPEWPATLSPAILQNLLRQELGFTGVILTDSFSMGAIRRL
                     YDPAEAAIRAINAGADMIMLAEERYGAKPEDYLRGQITLIEKVRAAARGGVIPPARLN
                     EAVNRVLALKAAAGLFPTAHPRREEAVRVVGCAAHRAVELEAARAAVAVVRNRDGRAP
                     LALAAGSRLVVVSPVDPAAYQIFSKMRGIGPNIAEPPAKTLFQQIQRRHASSDLVTIV
                     DAGAISRYREILRSAAAVIVATENYPLPGFRFPAECQRAVIEGLLAAGLDPVIIGLRD
                     PYELLDLPAVRTYISAFGYAPVCSQAAVEVLFGERPAEGRLPVEIS"
     gene            10358..11047
                     /locus_tag="VFA54_04045"
     CDS             10358..11047
                     /locus_tag="VFA54_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZS50008.1"
                     /translation="MSPAPNLISFAVVPEDPLAMLPRTPIRQFEKNTVIYDRDHQREG
                     LHLIIEGRVKVSRIAEGGKEVVIDFCHKDDFFGDMCFLGIRQDSERATAVDKTSIMLW
                     TLPELERLMARTPALGPALLSVLAQKLANANNRIESFCVDPIHRRLAKALLHMADRFG
                     QQADGNLIHLPPLTHEQLAWYVGTSREMVTQYMNQFRRDHLLKYSRRGMDLDVVALRK
                     YLGGALYQMVA"
     gene            complement(11149..11310)
                     /locus_tag="VFA54_04050"
     CDS             complement(11149..11310)
                     /locus_tag="VFA54_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HZS50009.1"
                     /translation="MLWYAWVFLVIAIIAGLFGFGLVASAAAGIAKVLFFIFLIVFII
                     SLFMGRRAA"
     gene            11938..12750
                     /locus_tag="VFA54_04055"
     CDS             11938..12750
                     /locus_tag="VFA54_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50010.1"
                     /translation="MIDLRTGVEHDRSRQTGGLNTMTRINAILGALLCLLYGSFVIPC
                     QADEWNEKTIVTFSEPVEVPGAVLPPGKYVFKLADSQADRHIVQIMNAEENHIYATVL
                     AIPSERMRPTGKTEMAFYEMPAGQPKALKTWFYPGNNIGQEFAYPKARASEIAQVTHE
                     NVPSAPEEAPQAGATLHGGTEASANPSPAPAPAERPGAAPSQPVETAQANRPAEANPP
                     AAQANPQAETTPLSRAPERMPRTASPLPLVAGVGILLAGAGLVLRRLAKGIA"
     gene            12776..13813
                     /locus_tag="VFA54_04060"
     CDS             12776..13813
                     /locus_tag="VFA54_04060"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HZS50011.1"
                     /translation="MNDLAQRRTKTKVPLRWAARFSLGAGLFLLAYCGFQILGAALWQ
                     RYQSWKLDRSILNGNAPIYSRAPQSAHTGMQQRFPYQATEGAPGIEEQRESSPVSRQP
                     LDAAGPSPSKRPGRDEAASPGVIGRLSIARLGLSAMIRDGTDDETLSLAVGHLPGSAL
                     PGEPGNVVLAGHRDTFFRSLRNAQKNDKIVISTPRGAHEYVVDSMMIVDPQESQVLRP
                     AVQPTLTLVTCYPFSYIGPAPQRFIVQAKEVGGAGNAGLLPQPSAAAGHSPTPPRAAE
                     AAKPALLLVPETPQGKGMRRSKSAALSERPAFTYREPDPPPATSQKRHGKWARAFRPF
                     GKLARAIAHPL"
     gene            13893..14390
                     /locus_tag="VFA54_04065"
     CDS             13893..14390
                     /locus_tag="VFA54_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50012.1"
                     /translation="MKAEISYYVSVSALDSFRVAAAFGSACMAGMMNSVAGGGTLVTF
                     PTLIWLGLPSIAANATSTVAIWPGSLGSMWVSKGTAGLGSSYLSLLFLIQKPVQERMK
                     KTGGLARHSPRWMAAAMLFQLFVAAHGGYFGAHRYFDAGGPECFRSYKYSSNEWLKKL
                     FRSVH"
     gene            14653..16449
                     /locus_tag="VFA54_04070"
     CDS             14653..16449
                     /locus_tag="VFA54_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZS50013.1"
                     /translation="MRSILQSLIKRSTDSLAILLAGLLVACVTASYLTRDQGSNRAGA
                     RQPPGRGQAPLVDYHLWQTARQLASQADTPEEQDFAREALRLADHELDQAFATELREA
                     ALSAPPRGPLKELADRVTRLKSRVAAGQARIAQLTKEAASKDSAIDQLELAKAQLALD
                     QDELEDAQQDLARQGGDRHSKLERALQKHEAAQQQPAALPKVSASASPITLQQQLALW
                     MSLRARERQLLAAQQQAAAKASALLREHEALDKLVNNKTIPASAEGSAPSGDREDEDT
                     QAMLARLHRLSDQRKSLSELDKRIQDAQQLGENYRSWIDAVEGRRRGVLHLLLNSVAA
                     VLAILIASLLLSKGIRHAFGRQTDRRRLRQLRVIASIAVQLAAAGVILLIVFGAPSQT
                     STIIGLATAGLTVVLKDFIVAFFGWFALMGRNGIHVGDWVEIEGVSGEVIEVGLLRTV
                     LLEMGNWASTGHPTGRRVAFANSFAIEGHYFNFSTAGQWLWEELQLPLPAAGDPHEIA
                     RKIQETIERETAADARLAEQDWERVTHQYDLRPFSASPAVELRPSASGVNIVVRYITR
                     APQRYEMKTRLYHEILDVLHKPAPSPASPVKA"
     gene            16646..19036
                     /locus_tag="VFA54_04075"
     CDS             16646..19036
                     /locus_tag="VFA54_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZS50014.1"
                     /translation="MSRIIRKAAVLGAGTMGARIAAHLTNAGVETLLLDIAPDRLTDA
                     EAAQGLTLESKTVRNRIVRQLFEAMQKSRPPALFLPALASRIRTGNFADDLPKIAGVD
                     WIIEAIVENFDAKRALLAQVDNYRKPGTLVSSNTSGLPIASLAEGLSADFRAHWLGVH
                     FFNPPRQMRLVEMIPTPETLPEVIDSLRDFCDRRLGKVVVFAKDRPNFIANRLFLFGL
                     MDLLKSMQKHGLTIEEVDALTGPLIGRPNTATFRLADFVGVDVCVFVGENLYRLVPED
                     EQREVFSPPEFLKRMVEKRWIGDKAGQGFFKREGKERLVLNLETFEYGPPRPVDLPSL
                     EAAKQIRDLGARLRFLLSQQDRAGRFLWDTWSRFLLYAAARIPEITDDVPSIDATMRY
                     GFNWEKGPFEIWDAIGVPESTQRMEAEGKAMPPLVQKLLQSGGASFYKELQGDRFYFD
                     LASVSYRPLSPRPGVTSLAALKQRNKILRSNPGASLIDTGNGILCLEFHSKANTLDRD
                     VISMIRSGVEETESRYEGLVIGNEGQNFCAGANLQWLLELCRSQRWDEIDQAVRATQQ
                     AFLALRDCRKPVVAAVFGQTLGGGSELAMHTSRVQALAETYMGQVETAVGLIPAAGGA
                     KELLWRWMGTIPADADPTPYLKEVFELIAMAKVSSSAAEAAEWRLLRSEDPVTMNRDR
                     LIEDARQTALAMAQAGYAAPAAPAMIPVMGRAGLAYLKMSIYLARRAGYATEYDGHVA
                     TKLAYVLSGGDLAQPMSVPESYLLDLERQTFLSLCGEAKTQERVEYMLRTGKPLRN"
     gene            19471..21882
                     /locus_tag="VFA54_04080"
     CDS             19471..21882
                     /locus_tag="VFA54_04080"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZS50015.1"
                     /translation="MPLPLTYPEPKRDLSEFTRLDNRLDVALAGFEQLALREDSPVTA
                     IRRIPARQPDWAEMPASLSPALADVLRKRGIARLYSHQAEAFERIRAGENLVIVTPTA
                     SGKTLCYNLPVLHYLIENPSGRALYLFPTKALAEDQLHEFHSLVEEMGSDLRAFTYDG
                     DTPQDARRAIRERANVVLTNPDMLHSGILPHHTRWAKLFENLRYVVIDELHTYRGVFG
                     SHVANVLRRLKRIAAFYGSQPQFICCSATIANPRELAQALLEQPVQLIDRNGATSGEK
                     FIVFYNPPVVNRQLGIRRSYIHEARNIARGFIERGQQTLVFANNRLATEVLVTYLKDA
                     CENGPGRAASVRGYRGGYLPKERREIERGLREGKIQAVVATNALELGIDIGSLDVVVM
                     AGYPGAIASTWQRAGRAGRRQALSAAVLVASSAPLDQYIIEHPDYFFSQSPEHGCINA
                     DNLEILVNHLKCAAFELPVRSGESFGGRPTEDLCKFLEELGVLHFAGNAWHWTSESYP
                     ADAISLRKVASDNFVVIDVTAEPKVIGEVDFSSALTTLHEKAIYLHEARQYHVERLNY
                     EERKAYVRSVDCDYFTDAIDYTHVKILDTFESEPLGDKPPEVNSGSQAGGFSAPPAAR
                     KAHGEVRINRQIVGFKKIKFYTLENVGAGQLSMPEQEMHTTSFWLSFSQPLLERMPDF
                     TPSERQSGLAGLGNVLRTVAALLLMCDPRDLGVAVTEDPAGSGNFERPQFEPNLYLYD
                     NYPGGIGQSEPLFRMCAELLAKSRELIEGCPCKAGCPSCVGPIEEVGARGREAALRYL
                     AELGA"
     gene            21882..23210
                     /locus_tag="VFA54_04085"
     CDS             21882..23210
                     /locus_tag="VFA54_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HZS50016.1"
                     /translation="MTSDLKSQLALLRRRITRINRKYQGGPAGTSPPGSLDGTFALYG
                     GSDAFSPGDSACEPEIYAPVRYSPEKFVPGSVVENEQGRYFLAEKFFPSHRHHGGIEF
                     SRLNELAGELLPAISPDDLPACAPGRWAFLDTETTGLSAGTGTCAFLIGVGTIEPAGF
                     RVRLFFMRDYDEEAAMLAGLASLLSRFEVLITYNGKAYDAPLLESRYCLRRMASPLSR
                     LAHLDLLFAARRLWRLRLESCRLIQLENEILGVERQGDLLGEMIPYHYFEYLRTRQAF
                     RLAPLFHHNTMDIVSLAALTAIVLPAFAAPEGSGLRHGEDLLGLARWLRQNGRLPEAV
                     TLYRRAIQRGLSDRDLFSALWEMAILEKKSGHRDRAVAIWSDLAFTQNAFRSAALEEL
                     AKYYEHTERNYSLALEMTAAALALAPAPALERRQRRLKRKAAEQSRPLLR"
     gene            23493..24269
                     /locus_tag="VFA54_04090"
     CDS             23493..24269
                     /locus_tag="VFA54_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HZS50017.1"
                     /translation="MIAKLAILFLLAAPWAWSWGQEGHRITAQIAERHLSSKALAAVR
                     QILGPGRRLEDIANIADEVRTRRPETAPWHYVDIPITARSLDRGKYCPNDQCIIGAIE
                     RFWRELAAPGLSPERRYEALYYFVHFVGDLHQPLHCGDQGDRGGNDVQVKFQGELMNL
                     HQVWDSGIIKSMQLDGEKLRRLGKISRRRRRAWARGTPEQWAMESHDLARDVAYRFPE
                     PVKDSVMLDQNYERRAASVVRTQLAKAGYRLAYALNQIYR"
     gene            24379..25587
                     /locus_tag="VFA54_04095"
     CDS             24379..25587
                     /locus_tag="VFA54_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HZS50018.1"
                     /translation="MRTTGVGRYLGALVLAVVWGGLAPAGKAQTELNFSRMGGVARIV
                     NTDMAVLEAGDKRDDLPCEVSPMKPELGFDLRFHTSYQVTIPLRELAGSGDQLRVLIR
                     VTPVDQPANQIYLVDRFNVPPIDEDAKGEATLPGGFTMGPGRYKVDWLMRDRTERVCS
                     THWEAEAKLDNDNKDLPLIMPPNTVAQRPQEPFSEQPPVERVQSGRLLHVKVLVNFSP
                     TNPREASLKPWDIEAIVSILRSIAREPQIGRFTVIAFNMQEQRVIYREDNVERINFPA
                     LGEAVSGIHLGTVDYRRLQDPQSGTRFLTSLLTEQLGPQNPEPDAIIITGPKLMLEKK
                     IPSDVLKEAGRANCPIFYLNYNFNPRNNPWRDAIGSALKFYRGLEYSISLPHDLGSAL
                     TDMMFRLSKR"
     gene            25647..26609
                     /locus_tag="VFA54_04100"
     CDS             25647..26609
                     /locus_tag="VFA54_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50019.1"
                     /translation="MLAAALLLAPMAAGQPKEPLKYELAGRVRPQGRASLSLHSTTSP
                     FMCSSQSDPAGRFRFRGLPAGTYTLAVLLPGRGEARQTIEVGPKSAGPKRRVEVTVDL
                     TDPKVVSIEALQRRAVVSAKELSIPERARREYAEAQKKLAEPDVAAAVAHLEKAVQIA
                     PQYWIAWNSLGTIAYQSHDFSRAESCFREALKQNPAAFEPLVNLGGALLTRGKWNEAL
                     SYNTQAVGVRPHDALANAQLGMNLYYLGSLDRASEYLAAAKRIDPRHFSHPQLILAEI
                     DVRRHDRQAAARELEDFLRVHPDWPGAESIRRNIDRLLMAELSE"
     gene            complement(26859..28502)
                     /locus_tag="VFA54_04105"
     CDS             complement(26859..28502)
                     /locus_tag="VFA54_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZS50020.1"
                     /translation="MNQNENLPESSFGELLQAFEGSRHFGAFGPDEPRVGIVAAITGE
                     QVFVDIGGKSEGVIPVADLRNPDGALKVQVGDEIQVTISGRDSEGYLLLSAVAARRPQ
                     DWSALERAFQSREIIAGRVTGVVKGGLSVDVGVRAFLPASRSGCREAAEMEKLVGEEI
                     RCRIIQLDIEDENVIVDRRGVLEEEARTARRRFLESLREGAVVRGKVRSLTEFGAFVD
                     LGGVDGLLHVADMAWSRITDPKAVVSPGDEVEVKILHIDRDKPRIALGTKQLTPDPWT
                     EIGQKLHPGDRVRGPVTRVMDFGAFVEIAPGVEGLVHISEMSWAKRVRRPGDLVKTGD
                     VVEAVVLGVNAAEKRISLGLKQALGDPWEEAAKRFPPGTVVEGTVRNVQKFGAFVELA
                     DGVEGLLHVSDLASDRRLNHANEVLKVDQRVRTVVLEFDREKKRIKLGMKQLEPDSSD
                     EYISEHKVGDEVTGRVVHIENGAVRVELGEGVIGICRAGDAPPAATGSFGAQLAAALG
                     KRDTPGPQGVAREALQVGQVRSFRITGLDAASKRIDLAR"
     gene            complement(28856..30136)
                     /gene="eno"
                     /locus_tag="VFA54_04110"
                     /pseudo
     CDS             complement(28856..30136)
                     /gene="eno"
                     /locus_tag="VFA54_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            complement(30180..31433)
                     /gene="glgC"
                     /locus_tag="VFA54_04115"
     CDS             complement(30180..31433)
                     /gene="glgC"
                     /locus_tag="VFA54_04115"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZS50021.1"
                     /translation="MQDVLTILLAGGAGERLWPLTRERAKPAVPFGGTYRIIDITLSN
                     CINSGLRKVYILTQYKSLSLNRHLRRAWNILSPELGDFIEVLPPMKRVSENWYLGTAD
                     AVYQNLSSILEEATPYTLVLSADHVYKMNYGRLAAWHRDHKADVTLATIQVPPSEAHR
                     FGIAEIAEDGSIRGFEEKPQHGSPERSRFNPEMCSASMGVYLFSTEVLAEALRRDAED
                     AGSTHDFGRDILPWLAREARVFAYDFIDENKKEVRYWRDVGTLDAYYEAHMDLVSVSP
                     VFNLYDTSWPIRTFLAQYPPAKFVFADEGRRMGVALDSLVSHGCIISGGRVINSVLSP
                     GVRVNSFSEVEYSILLPHVQVGRRSRIRRAIIDRGVVIPERSEIGFDLEQDRRKGFVV
                     TDSGIVVIDRETMAQQMAEAPQRIG"
     gene            31701..31898
                     /gene="rpsU"
                     /locus_tag="VFA54_04120"
     CDS             31701..31898
                     /gene="rpsU"
                     /locus_tag="VFA54_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZS50022.1"
                     /translation="MAEVRLQEGESLENALRRFKRKVQQEDIIKEVKRHSFYLKPGEK
                     KRVKQALARKRNRKKSRREVE"
     gene            32006..32329
                     /locus_tag="VFA54_04125"
     CDS             32006..32329
                     /locus_tag="VFA54_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HZS50023.1"
                     /translation="MPEFVDRILKCTECGADFVFTAGEQHFFYDKNFKNEPKRCKACK
                     SKRTHGGSHPGSHKVETVAVCSQCGKQTTVPFKPTQGRPVFCRECFLQRRGVAATGGV
                     SSASA"
     gene            32401..33861
                     /gene="mtaB"
                     /locus_tag="VFA54_04130"
     CDS             32401..33861
                     /gene="mtaB"
                     /locus_tag="VFA54_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZS50024.1"
                     /translation="MAFVGSPASFFVQNFGCRAAQADGAALEAALQAKGFAPAGRAAA
                     ADVVVLNTCTVTAAADEDARQAIRRIHRENPRARILVTGCYAQRAPLELAALPGVSWV
                     VGNSHKHLISDLVPIQPLLNQPGPADGEPPAAAPRSVKGGLPQPVKPLDNLSEECGPD
                     NLEFHGQILVGDISSQHRFLPVLGSGASEASPAVTFDRTRPILKIQDGCDSRCSFCII
                     PSVRGRSRSAPPELVLEQARVLASQYREIVISGINLGRWGQDLPGRPRLAGLLRVLLE
                     ETSAARLRLSSVEPMDWTDELLRLMAGDRRMARHIHAPLQSGSDRILRKMHRRYRAHQ
                     YAERIEKAYTWMPEAAFGADVMVGFPGETEADFEQTYRLIEELPFTYLHVFTYSPRPS
                     TPAAELADQAPAPVKRERNRVLRELAQKKGEAFRRRWVGKRLSAITLDDSHPAGAVAL
                     SENYIKVLIPGVRLPGNQIVEVEGAGLDGEFLTARN"
     gene            complement(34143..37376)
                     /locus_tag="VFA54_04135"
     CDS             complement(34143..37376)
                     /locus_tag="VFA54_04135"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS50025.1"
                     /translation="MRKSVLSIVAALAAFAGNAMAQQSGQIVGSVRDSSGAVVPGAAV
                     TVTETGTGFSRSAVTGADGHYVLPNLRPTEYVITSESAGFRKFSQSGVELLANQSLTV
                     NITLEVGAVTETVSVAASAVQVDTTTSTLSEVVDQSRIIELPLNGRDAAKLSTLVAGT
                     AVISVSSESGKSIPGGLQLSSNGSRTAQVAYKLDGTTNTDPYFQENQTFPFPDALQEF
                     SIQTSNYSAVSGNNAGAVVNVVTRSGTNQLHGGAFEFVRNRVLNARNTFSPTQDFLKR
                     NQFGVYGGGPVVLPKLYNGRNKTFFFMGWQGTRIRNLANSLSAFAPTIDERNGDFSTC
                     GKPCNVALKDPLGGTFPNNRIPVSRFDPSSVKVNSHIPAVGGDGFTVIPRPINWQQDQ
                     GVLKMDHQLTGKDRLSGRYFIDHFLNAGTYDPSNLLSYRNPTLASRVRSQSAVLTWLR
                     TISPAVVNDFHFGFNRIHAARGPYFSGVPSMQDLGVRLPIYPTLPSISEINAVGFFNI
                     GDNLEAKFPRTGFEWANRTSWVHGSHTIQFGGEINRQRADIANQFRRAGHFQFSGDST
                     GLAMADYFLGVIRSFDQGTGEYKANRDTYPAVYFQDDWKVRRRLTLNLGVRYEPTPPW
                     HEERGRIEIFRIPDFLNGVKSTKFVNAAPGETFRGDPNAPEDGTLGDYNNVAARLGFA
                     WDVFGDGKTSVRGGAGMFYDQHLEGDFNNNAVNVPPWSIRLSVTQPEGPFSDPYRGRS
                     DFNLVRVESIGDPNAPFPRPVAISTYDPRQETPLAYNWNLTLEREVFPEWLVRAAYVG
                     SASNYGRTTVQLNPAVYIPGSSLGTDARRLFAPTIGGVDYYTQDRRSYYHSFQLSVTK
                     RFSRGFTVLANYTLSKSLDDYGDKVMPWYFPHANAMQYGPSDFDHRHRFVVSWVWQLP
                     NVVPQNGFGRWVLNGWQWTGIGQYQTGAPFTIKSGKDNSLTGLGNDRAKLTGAGITPP
                     AGADKRVWFNSAAFAVNDIGTFGTLGRNTLYGPQLYSWDMGLFKNFRVNERFQTQFRA
                     EFFNIFNQTNFDNPVAQVGAGGFGSITRTNPAAGDPRIIQFGLKLTF"
     gene            complement(37645..40932)
                     /locus_tag="VFA54_04140"
     CDS             complement(37645..40932)
                     /locus_tag="VFA54_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50026.1"
                     /translation="MGTQRVVDPAAVLPAVLFLTVLSIAAFAQTQKATILGTITDSSH
                     AVIPGAQVVVTEINTNVRRTETTNDSGFYVFANMDPGTYRVEVEQPGFRRMVRSGIDL
                     TPNTTARVDLELSPGAVSETVDVTAEAPLLQTDRADTGGKIEEVQLQTMPLGFNRNYQ
                     SLIALVPGVGRPFRPHSEFYNSQDSLGARVNGMGRQLNNYQLEGIDNNIDTGNLTAIV
                     LPAEAIATVDVSTSNYDPEFGRAGGAVTNVTMRSGTNEFHGSLFEFHQDRAIRATQPF
                     ATSKPGMVYNQFGGTLGGRIIRDKLFFFGDYQGSRDHFGSSNLATIPTMAFRQGDLTA
                     SPTPIYDPATGNADGTGRKEFPNKQIPLNRVDSQVQKILSFIPPPTSSGLQTNFQKNT
                     VRVKNLDQFDIKIDYVIGPNDRLAGRYSYQVAFVDDPGLYGPGGIYGGPHNQGFSGTG
                     PARNQSPSLSYSHIFSPTLVTEVRLGYVRDRNDAKNVDYGLTTSKDIGIPGVNLDAWS
                     SGLTQIVVNGFGPELIGYDGSLPWARSVTNYGIVNNWTKTMGNHVIKWGADIRRERND
                     LQQTDPPRGQFTYTEGPTLLNGGSGGLSFGNSFAAFLLDQANKINRGLAVFFPARREI
                     LYNLYFQDKWQVTQKLTLDLGLRWEVWPAGTPHFPAGYSNYNPTNNTLELAGVGSIPN
                     NLGIDGKWLSFAPRFGLAYRINDKTVFRGGYGISFLPRVTGPMNFPVKQANNFTAPNS
                     FAAAGSLATGIPAPDIVQIPSSGILPAPLNQNYSIVPKDLFHGYVQSWNIALQRALPG
                     NFALDVAYVANHGVNVRASEQINYGRVLGGGPAYQPLNQLFKRTNSTTTALGTHSYYN
                     SLQMRFDRRFSQGFMLTTSYTFSKAIDFCTDINCSLNNPMFVSLNRGRSDYDVTHVWV
                     QSFIYDLPFGPNKKFLHSGIGRWIFGDWQANGIFTSQTGRPLSITFSSSTLNTPFVNN
                     RPNLVSAGTPAVYHNVGKGILWFDTSKFSAPAPGTLGNVGRNILTGPGVVNLDFSLFR
                     NFSITERLKGQVRLESFNFTNTAHYDNPNTTFGDANFGQVTTAGGDYNTGRGDPRQFQ
                     IGLRLFF"
     gene            complement(41574..44546)
                     /locus_tag="VFA54_04145"
     CDS             complement(41574..44546)
                     /locus_tag="VFA54_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZS50027.1"
                     /translation="MFQRYTAIAALLLCCTTCLLAQGLNTRATKNDWEEINFEFNSSI
                     LSDGYPSLLRLAELLQTHPDYRVHIEGHTDFVGSEQYNDRLALARANTVRDFLVKYGA
                     RANQIQTSGRGKRNPEVDNRTPEGRFMNRRVVLTVTNGQGQQVSDGGVAEAIQAMTPP
                     AKPAPDCCSDILKRLDDIMAMLRDLKNENDDLRKQVAALKAQPAPPPAPALPPPVSKE
                     ELSNLASEAAKQAVEAGRTRRFSLLGLNAGPDTNGNTTFTGKGRFFAPFSNDSPHAVQ
                     IEGEYLYFRDRQEGQFDAGLVNRYKSFQAGLFSSFKHVSFRNFQSGGTLGEGALTLDY
                     VFGRGRVGAFGTKGFLNNAVVNETPLGNSLIQQTYLRIVDQVGFSTQVGLWRSAYVEG
                     NLGALFQRGAENRPGGTLRFVQPINRFWAFTVEAGLNETLAGKDNTGRVVFGIQLGNW
                     LRPREYAEVRHPVPVDIPRLRYEVLTRTVRSGNDPPVADAGPDQINVPPGTITLDGSG
                     SYDPNGDPITYQWTQTFGPPVTLSNPTSAKTTFQADAGKQYSFRLTVADNHGGTGTAK
                     VTVTTRNLAQAQIVKFTADPPQITAGQTSTLIWQVEGADRVTITPGIGQVDSKAGTIS
                     VAPTATTIYTLTAQNSSGEVTQTATVTVIRPDARIVSFQATPTNIQPGGAATLSWQTE
                     NADQVSISGIGNVAKNGSTIVSPNQTTTYTLTAQNRFGSVNATVTVTVGAPGVPRILQ
                     FSASPTAVLPGGQATLVWQVENATSVTITGIGSVQPTGTSTVSPTTDTTYTLTASNGQ
                     GQVSATAVVTVIQAVKILNFTANPPAFQKPGDPITLSWQTSNATQVIITGVGSVPVNG
                     SVTVNPTVATSYSLIAYGRRSQVTAIVVVNPANGGAGGTGRAPVANPGQDRRVQSATV
                     LLDGSGSYDPEGGSLTFSWTASGSKLVRIDNANSARATATLLSGPDPYPGEYDFQLTV
                     TNSKGVSTTVPLRIYYIP"
     gene            complement(45011..46369)
                     /locus_tag="VFA54_04150"
     CDS             complement(45011..46369)
                     /locus_tag="VFA54_04150"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZS50028.1"
                     /translation="MTRDEAQQLTKKILSFATVPEIQVSVVAGRRSHLRFARNSPTTS
                     GTSDSTAITVIAWKGKRKASASGTLSAQGGNTDSALQALVAEAEALAALSPEDPEYMP
                     LLGPQKYLEVKAYDAETAEMPAAARAKAVGEAVALARSKKLVAAGILRNYGVYHALAN
                     SAGLFAYFSTSTASFSITARTADGWGSSYAEASSVSMRSVDCRETAAIAVKRALDSRG
                     ARELPPGDYPAILEPQALADMATSLLFALNARPADEGRSVFSAPGGKTRIGERIFDPR
                     VNIYTDPQHPVVPASSFSGDGFPTAKAYLARDGVLENLSNTRYWAKQKGRPPGPFFVN
                     LIMDGEGKPVSEMIAATERGILLTRLWYLRMVDPQQALVTGLTRDGTFYIEGGKIQHP
                     VRNFRFNESLVRLLREVELLGTPQRVMQSENISFGDGGMRMMLPAIKVRSFRFTSISD
                     AV"
     gene            complement(46534..>47480)
                     /locus_tag="VFA54_04155"
     CDS             complement(46534..>47480)
                     /locus_tag="VFA54_04155"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZS50029.1"
                     /translation="SDCSFTVTAVDSARGEFATRSSLAYPAERGYEWIEEYPHLAEAE
                     QAAEEAVRKLAAKPVAPGKYDLLLHPTNLFLTIHESCGHPTELDRALGYEANFAGTSF
                     LTPDKLGKFRYGSKWVNMIADRTQPYGLATVGYDDDGVPAQRWYLIREGIFVDYQTTR
                     EQAALLPGMRSHGCAHADSWSSVVFQRMPNVSLDPLDSKLSQEELMAGIKDGILILGM
                     GSYSIDQQRYNFQFGGQVFWEIKNGKKGEMLRDVAYQARTPDFWNSCDGVGSRTEYIL
                     SGVFNDGKGEPEQLNPVSHGCPPARFRQVNVINTGKKS"
BASE COUNT         9243 a        14534 c        14375 g         9328 t
ORIGIN      
        1 gagccgctcg gcatgccggc atacatccgt ggcgtgcatc caacggcgat cggcaatgtg
       61 atgctgaccg gcatcaacgt cccgatccgc atcggcggag tgaccgtaat gcccggcgac
      121 gtggttttcg gcgacaacga gggcgtcacc ttcatcccgc cgcatctggt gcagcgcgtg
      181 gtggaaaggg ccgaagtgac gcaacttcac gacgagtgga ccaaggaaaa gctcaggacc
      241 ggcaaataca agtccagcga aatctactcc actccccgcg atcctgcgtt gcgaaaagaa
      301 tacgaggaat ggctggcccg cgagaaggcc aaacggggca tccggtgagc cggagctgga
      361 ttggctgaag gatcccggtt tcggccggga tccggcgcac ggacgctgtt tcgtcatgct
      421 ggcagcagcg cgggaggttg cgccgcattc atactcccgc gactaccacg caaaccaggg
      481 tggggcggga gaaacgtccg gcagcggcat cttccggacg ccggcaggaa accgatcccg
      541 gcaattctca gatatggcat gatctcgcgt ctcgcctctg ccgatatgaa gtggagtgag
      601 gatttcggct ccacaggcct ccgaagcggc gtcggccact ttggtcgggg cgcgtcgggt
      661 tgagcgggcg attgcccgcc tccaggcgtt gccggccttc tatcccccgc tgcaaaaggc
      721 cttgcggctg ctcgaagatc ccgattcgcg catcgattac attcaccagg tgatttgctc
      781 cgatcaggcc atggccagcc ggatcctgag actggtcaat tcagcctatt ttgcagttcc
      841 ctccgaggtg cgcacgattt ccctggccat cactctcgcc ggccgtgaca gaattgcggc
      901 gctgttgcgg cgcttcttga gcgaagaact cctggccatg ctttcaaacc gcaagccggc
      961 ggccggccat gtccgcgaga cttcgctggt gacggctgcg gcagcgtcct gtatcgccga
     1021 gcggctgctg cgatccgact gcgaagagat cctgctggcc gggttgctgc acaacatcgg
     1081 cgagctcgtt tttctctcgc agttccgcga gaattacgaa gaaaccctgc gcctggccgg
     1141 ccacatggcg ctcgaagaag ccgaaagaat cgtttttggc gtggagtccc gggtggcggg
     1201 caaatggctc ctggaagcgt ggaattttcc gcccccgttc ccggcggtcg cagagcactg
     1261 cgccgatccc tggggggtcc atttcgcggc ggcgcctctc gccgtgatcg taattgttca
     1321 tacggcacgc aagctcgccg atgcctgggt tgcagatgcc cgccgcgggc gggcgcagca
     1381 gggggcggcg gccgaggcgg ccagcgccat tgcgccgcgg gctctggccg tcctgggcgt
     1441 agaccgctgc tttttggccg acatctaccg ggatctgggc gagcagatcc agcgccggca
     1501 gtctctctgg acgtaaattt gtcaaaacac ggcttcctgg ccggctcgcg gcgagcggtt
     1561 gtgctatgct aggggagtaa taaagcgaaa tagaagctga gaaggagtac ggaaccagag
     1621 aatgccactg gcgaaagaag caaagcaaag tatcatcacc aagtatcata cccatgccaa
     1681 ggacaccggt tcgccggaag tgcagatcgc ccttttgagt gagcgcatcc agcaactgac
     1741 ggaacatttt aaggcgcata agagcgatca tggatcccgg cgcgggctgc tgatgctggt
     1801 gagcaaacga cgccggttgc tgacctatct gaaagcgtac gacgccgaac gatacaaagc
     1861 cttgatccag aagctgggaa ttcgccgtta gcattcagcg atcggttctg acattggaat
     1921 tggctacgcg acgctcagtg agagctgcgg ccagttgttg gctcaaatca aacgtctcgc
     1981 ctcttcacaa ccatccgctt ccatttcgct gatactaatc aaacagcagg ctgtcagccg
     2041 cgggccgtgg tttggccgcc ggtgaatccg tttcggggac agcccgcagg agaaagtaat
     2101 agatgacgca aaccacgcaa gtcgaagtcg gtggacgtac actgacgctg gaaacaggca
     2161 agatagccaa acaggcgaac ggcgccgttc tggtgcgttc gggagactcg gcggtcctgg
     2221 tgaccgcggt cgcggcccag gagggccggg aaggaattga tttttttccg ctcacggtgg
     2281 attaccgcga gtacacgtac gcggccgggc gcattcccgg cggatatatc aaacgggaag
     2341 ggcggccgag cgagaaggaa attctcacca gccggctaat cgaccgcccc attcggccct
     2401 tgttcccgga gggcttccat tgtgaaacgc agatcatcgc catggtcctc tcggcggacc
     2461 ccgagtttga cccgagcgtg ctgagcatcg tcggggcgtc agcggcattg gcgatttccg
     2521 atatcccctt tttgcacgtc atcggcgccg tgcgcgtcgg cttggtggac ggggagctca
     2581 tcgccgttcc gggatacgca aagctaaagg aatcgaagct caacatcgta gtggccgcgg
     2641 ccgaagaggg catcgtgatg gtggaggcag ccgcatcgga agtggacgaa gccaagatga
     2701 tcgaagctat caacttcggc cacgaaaact gcaaaaagat cgtagcggcg atccgggaac
     2761 tggccgaaaa agtcgggaaa ccgaagcgca aggttacgcc gccggctctc gatcaggcgc
     2821 tctacaccga aatcgaaacc aaattcgggg cctcgcttcg cgatgctctg gacacagcca
     2881 agcatcccaa gcaggaaagc catgaactcg tacgcgagct gaaaaaccag gtcctggccg
     2941 gtttcgccga gcagccggag aaggccgcgg aagccaagaa gatcttcgaa gcgttgcagg
     3001 aaaaaatctt ccgggaggac gtgatcctgc ggcgccgccg gcccgatgcc cggggcttcg
     3061 atcagatccg gccgatttcc tgcgaagtgg cggtgcttcc ccggactcac gggtcggcgc
     3121 tgttcactcg gggtgaaacg caagctctgg taacagcgac gctggggacg aaagaggatg
     3181 cgcaacgcca ggagctgctg gaagaagcgg agagcttcaa gcgattcatg ctgcattaca
     3241 atttcccgcc gttctcggtg ggcgaggtcg gttttctgcg ggggcccggg cggcgggaaa
     3301 tcggccacgg ggcgcttgcc gaacgcgccc tggccccgat gatccccgag gaagaggatt
     3361 tcccatatac cgtacgcgtg gtcagcgaca ttctggaatc caacggctcg agctctatgg
     3421 ctaccgtatg cggagcgacg ttgagcttga tggatgcggg cgtgccgatt aaggcgcctg
     3481 tggccggcat cgccatgggc ctggtgaaag agggggataa atacgcggtg ctcaccgaca
     3541 tcgccggcgc cgaagaccat tatggagata tggacttcaa agtggcgggc acgcgcgacg
     3601 gcatcacggc gctgcaaatg gacatcaaga ctccgaacgt gtcaacggag atcctcgaaa
     3661 aggcgctggc gcaggcccgc actgcgcggc tgctgattct ggacaagatg ctgcaaacca
     3721 tcgccgagcc gagagtcaag gtttctcctt acgcgccgcg gatctacacg atgaacatcg
     3781 cgacatccaa gattcgcgac gtgatcgggc cgggcggcaa ggtaatccgg gctatcatcg
     3841 aacagaccgg ggtgaagatc gatgtcaacg acgatggcac gatccacatc gcgtcgtccg
     3901 acgaggcgtc ggctaacaag gcgatgcaga tgatcggcga catcacggcc gtggccgaag
     3961 tcggcaagac gtatctcggc aaagtggtgc ggctcgtcga tttcggggcc tttgtggaga
     4021 ttttcccagg caccgacggc ctgctgcaca tcagcgaggt ggcggagcac cgcattcggg
     4081 atgtgcgcga tgagctgaag gaaggcgacc agatcctggt gaaagtgctg gcgctcgagg
     4141 gcaacaagat caagctcagc cgtaaagctg ttctgcgcga gcagcgcgaa aagaaaaagc
     4201 agatggagac tcaggccgcg cattagccgc cgcgggactc gcaaagcgcc gggcggcgag
     4261 cgtcttcgct cccgggccgg agccgggcac aaccttttcc ggcctctctc atagcgggag
     4321 ggccgcgact cgcctggtcg cggtccgagg acgttgcggt cctttgtgag catgacatct
     4381 gtctggcgtc aaaacccgaa gcagattcct cgcggcgccg ggagcgcccg gggctccgcg
     4441 ccggtcagtg gctgaggcca tttcagagcg ggtatcagcg attcacgttc tgcgcccgct
     4501 tacttcaccc ctgcttttcc gtctaagggc tcctgttcca ccgctgtctt ctcatgcggc
     4561 ccgattttcg gagggcgccg gactgcacag ccgggcggct gcccgcgcca agccgggatt
     4621 aaagtctccc gttttgataa actttgtccg atgtggagat cccgcatcgg tcaagctctt
     4681 cggggcagaa aacccagccg gaagtaacaa aaattggcag gcaacgcctt agctagaggg
     4741 ccccgcagga cgagactgtg aacatttccg aaactttcat ccggcgcccg atcgccacca
     4801 gcctgctgat gttcgccgtg gcccttttcg gcgtcctggc gtacagcgct ttgccagtgg
     4861 ccgatctgcc gacggtcgat tttcccacgc tgtctgtatt cgccagcttg ccgggggcca
     4921 gtccggatac gatgtcgtcg gccgtcgcca cgccgctcga gcgccagttc accacaatcg
     4981 cgggcctcga ttcaatgatc tcggtgaact ccacggggag cacccagatc acccttcagt
     5041 ttgatctctc ccgcacccag gacagcgcag cagtggacgt ggagacggct atcgcggagg
     5101 ccatgccttt actgccgccg ggcatgccgt cgcccccttc gtttttcaaa ttcaaccctg
     5161 ccgacgcgcc ggtgatgttt ttgaccgtca gctctcccac attgccgatc tgggtgctgg
     5221 acgattacgc cgagaaccgc atcgcccaac ggctttcgat ggtcagcggc gtggcccggg
     5281 tcaacgtttg gggagcgcag aagttcgctg tccgggtgca ggtagacccc aacaagctgg
     5341 ccgcccgcaa catcgctctc aacgacatcg ccgccgctct gcgtagctgg aacgtcaatc
     5401 tgcctaccgg cacgctctac gggccgcacc aggccttcaa cgtcctggcg aatggccagc
     5461 tcagcgacgc cgccgcctac cgccgggtgg tggtcgccta caggaacggg gccccggtgc
     5521 gtctggagca agtagccaac gtaatcgaca atgtagagga caacaagcag gagtcctgga
     5581 tctacgaccg ggaaggcagg cgaaaagcgg tcaacctggt aatcttccgg cagccgggga
     5641 gcaacaccat cgaagttatt gaagcgatca aaaaagtgct tggagagttc caggcgcagc
     5701 ttccgccttc cgtgcagctt agcatccgcg gcgacagatc gaagaatatt cgggacgcgt
     5761 ttcacgacat gcagtccacc atgattctga cgctggtgct cgtcatcatg gtgattttcc
     5821 tgttcctgcg aaacacctca gctactttga ttcccagcat ggcgctgccc ttctccattg
     5881 tggggacctt cgccgtcatg tacgtgctcg gctacagcct ggacaacatg tccatgatgg
     5941 ccttgatcct gtgcattgga tttgtggtgg acgacgcgat cgtgatgctg gaaaacatcg
     6001 tccggcatct cgagcacggc gagcgcccgc tggaagcggc tttcagcgct tcgaaagaaa
     6061 tcgggttcac gatcgtcacc atgaccgtat ccctggcggc ggtgttcatc ccggtattgt
     6121 ttatgggcgg gattttagga cgcctgttcc gcgaattcgc cgtaaccatc tgcgtggcca
     6181 tcctgatttc cgggctggtt tccatcagcc tgacgccgat gctgtgcagc cgctttttgc
     6241 ggcctccgaa tcaagccggc catggccgtc tttaccgcgc cacggagcgg ttttttcagg
     6301 gcatgttgcg cagctatgat ctgagcttgc gctgggtgct gcggcatcgt ccagtgatga
     6361 tcacgatttt ctttattgtt ctcggatcga ctgcgtatct gttcgtgaag gttcccaaag
     6421 gatttatacc gacgcaggat ctggatcagc tcaacgtctc taccgaggcc gctgaaggca
     6481 cgtctttcta ccagatggct gagctgcaaa ggaagatcgc cgatgtcgtc aaggcggatc
     6541 caaacgtgga ttccattctt tccatgatcg gaggcggctg gagcgggaac acggggaatt
     6601 tgtcggtgat gctgaagcct cgcaaagagc ggcgccttaa cgtgatgcag atcatcgagg
     6661 agctgcggcc caaactggcg acttttccca acattcgagc ctttctgacg ccgccctcgg
     6721 cgatccgcat cggcggccgg atgtcgaaaa gccagtacca gttcacggtg caggggccgg
     6781 ataccgccga gctctacgcc caggccgccc ggctcgagcg cgagatcgcc cgccttccgg
     6841 gtttgcaaga cgtaacgacc gatctgcaaa tcaagaaccc gcgaatgaac atcacgatcg
     6901 accgggaccg agcggcggcg ttgcaattga acgtggccga gatcgaaggc gaactttaca
     6961 acgcctatgg acccagttgg gcgacgacga tgtatctgcc caccagccag aaccgggtgc
     7021 tgctcgagct gctgccccaa tatcaggggc gtcctgactc tttgccccat ttatatttca
     7081 ggtccactag cggaaacctg gtgccgctca cgtctttcgc acggctcacc gaagatgcag
     7141 gcccgctcac gatcaatcac tccggccaac tgccctcggt gacagtttcg ttcaatctga
     7201 aaccgggagt ggcgctgggg gaggctgtcg ccgagattaa agaagtgggg gaacgaaatc
     7261 ttccggccac aatcactacg acatttcaag gagaggccaa ggcgtttcag gactcgctgc
     7321 ggaacctggg catcctgctg accgtagcga tcatggtcgt gtacatcgtg ctcggcgtcc
     7381 tgtatgagag ctacgtacat ccgatcacga tcctttcggg gcttccctcc gcgggattcg
     7441 gggccctgat cactctgctg ttgttcaaag tggacctcaa tatctacgct ttcgtcgggc
     7501 tcatcatgct gatcggcatc gtcaagaaga atgcgatcat gcagatcgac ttcgcgttag
     7561 aggccgaaag aaaggaaggc aagacgcccg ccgaagccat ttacgagggc tgtctgatcc
     7621 ggttccggcc gatcatgatg accacgatgg cggcgctgct cggagcgctg cctatctcgt
     7681 tcggctacgg ggccggaggc gaagcgaggc gtccgctggg actcgccgtc gccggcggtc
     7741 ttctgttttc ccagatgatc accctttacc ttacgccggt cgtttacact tacatggcat
     7801 ccattgtcca gcgctggaat gccagcaggt tcggaagaaa aggaagggcc gcagccgcac
     7861 agggtattcc ggcgctcggc ggacccgtcg attccccgct ccagcccgcc gccgccaaag
     7921 gcgattaggc agcttgagcg tccgatccca agctgaaggg gaacattgcg cgccgtttcc
     7981 tggcagccta cggaataacg gccatgaatt ccggagaccc ggcctgaagc ggagcacggc
     8041 ggccaatggt cattagccgc cgcgctgcct tcacgatatt tctacaggca ggcggccttc
     8101 ggccggacgc tctccgaaga gcacctccac cgccgcctgg gagcacaccg gcgcgtagcc
     8161 gaaggccgaa atgtatgttc gcacggccgg caagtcgagc aactcgtaag gatctcgaag
     8221 gccgataatg acaggatcga ggccggcggc taacagccct tcgattaccg ctctctgaca
     8281 ttccgccgga aaccggaatc ccggcagcgg gtagttctct gtagcgacaa tcactgctgc
     8341 ggcgctccgc agaatctccc ggtacctgct gatcgcgccg gcgtccacaa tcgtgaccag
     8401 atcgctggag gcgtgccgcc gctggatctg ctgaaacagc gttttcgccg gcggctcggc
     8461 tatgttcggg ccgatgccgc gcattttgct gaagatctga taagcggcag ggtccaccgg
     8521 ggagaccact accaagcggc tgccggcggc gagcgccaag ggcgcacggc catcgcggtt
     8581 gcgaaccacg gcgaccgcgg ctcgcgccgc ttccagttcc acggcgcgat gcgcggcgca
     8641 cccgaccaca cgaacagctt cctcccggcg ggggtgcgcc gtggggaaca aacctgcggc
     8701 ggcttttaag gcaagcacgc ggttcaccgc ctcattcagc cgcgcagggg gtataacgcc
     8761 gccgcgagcc gcagcgcgaa ctttttcaat gagcgttatt tgccctcgta aataatcctc
     8821 cggcttggcg ccatacctct cttctgccag catgatcatg tccgcgccgg cgttgatcgc
     8881 ccgaatggct gcttcagcag gatcatacag cctgcggatt gcgcccatgc tgaagctatc
     8941 cgtcagaatg actccagtga aaccgagttc ctggcgcaac agattttgca ggatggcggg
     9001 cgagagcgtc gccggccact ccggatcgaa tgccggataa agaagatgcg ccgtcatgat
     9061 catgtccacg ccggcagcta tagccgcccg aaatgggatc agatcgccgt ttctgatctg
     9121 ctgcgcgtct ctcgccacga cgccggcgcc ctggtggctg tcttgggcgg tgtcgccgtg
     9181 gcccggaaaa tgcttggcgc aagcaacaac accccctcga tggaggcccc tcacagcagc
     9241 ctcgacggcc tcggcaacac gcttggcatc cccgccaaag gaacgcgacc cgatgattgg
     9301 attgcggggg ttgcaattta cgtcggcgac cggagccaga tcggcggaaa tgccgagcac
     9361 tcgcagttcg cgccctataa cagcgaacat cgcctcgatg aggtccgaac gggcggctcc
     9421 cagcgccagc gctcccggtc cagcggtcga gtaaggattc aacacgctcc atgccccttc
     9481 ctgatcgcag gcggccagga tgggcagggc atgcggcgtg gccgcgccgg tatcctgcaa
     9541 atcgcacagc agttgcacgg cctgatcgac gccggtcaag ttgtcctggc tcaaatagac
     9601 gcttccgaca aagcaatccc gaagaagggg tttcgcttcc cgcgccgacg ccccctcaaa
     9661 cgccagaaga aacagttgtc cgaccttttc ctccagactc aagacgccgg gatccaacgc
     9721 gctgatctgt ttcatcgcgg cgctggctgg gaatgatagg ggctcagagc gctgattcac
     9781 gtcgttcgcc gcgcaagcat ccggcgatat cctcctgatt gtacccggca tcttgcaaac
     9841 ccgcggcggc aggctcctcc tgatgcaccg gcgtcgccaa agggggtgag ttgcggcaag
     9901 cggcttgcaa cgccatcgga agcgcatgcg ctgtggcgta gtggtgcgca cgccgggcac
     9961 aggagcttcg gatgggggcg aagtcttccc gccagtcgca aatacggtgg taatggaata
    10021 tcttacggga ggcgggccgc aacagggcgc cgccgctgct cgcagttttt cgatcgccga
    10081 acgccttggc gccccgagct gcgcccggtc gcttagtact ggaagtaaat taccgcaggt
    10141 gtaagaattt cctgtgtcga gtggggtgct gccgtgattt ccaccgatgc cgaccggcga
    10201 acattttacc agccgcggcg catggtttgc ctgccggtga cagaacttcc ctgcccgagt
    10261 gttccagccg ctcgacttgc attcgttgca tcacattggt ttggaaattg ctataaacca
    10321 tcaacttact gggatttttc cacgtcagga ggccctcatg tcgcccgcgc cgaatttgat
    10381 cagctttgcg gtcgtcccgg aagatccgct cgcgatgctc ccgcggactc ccattcggca
    10441 atttgaaaaa aataccgtca tctacgatcg agaccaccag cgcgaaggcc tgcacttgat
    10501 tattgaggga cgggtaaaag tgagcaggat cgccgaaggc ggcaaagaag tcgtcattga
    10561 tttctgccat aaagacgatt tcttcggcga tatgtgcttt ctgggaattc gccaggacag
    10621 cgaacgcgcc acggcggtgg acaaaacttc gattatgctc tggacgttgc cggagttgga
    10681 gcgtttaatg gctcgcacgc ccgctctagg tccggcgctt ttgtccgtgc tggcgcaaaa
    10741 gctcgccaat gccaataacc gcattgaaag cttctgcgtt gacccaattc accgccggct
    10801 cgcaaaagct ctgctccata tggccgaccg attcgggcag caggcggatg gcaatcttat
    10861 acacctgccg cctctgactc acgaacagct cgcgtggtat gtcggcacgt cgcgggaaat
    10921 ggtgacgcag tacatgaacc agttccgccg ggaccacctg ctgaagtatt cgcggcgcgg
    10981 catggacctt gacgttgtcg cgctacgaaa gtatctcggc ggggctctct accagatggt
    11041 cgcttgaaaa aggcgaagcc ccccgcccct tacactcctt gccaacgata agcgccgcaa
    11101 atgcgtgcgc gctgcccgag tgtggatcgg ttcaaagctg ttcccgcttc aagccgcgcg
    11161 cctgcccatg aataagctga tgatgaaaac gatgaggaag ataaagaaga gcaccttcgc
    11221 gattccggcg gcggcgctcg ctacaaggcc gaatccaaaa aggccggcga tgatggcgat
    11281 cacgagaaaa acccaagcat accaaagcat gatggttgtc ctttcaccag aactcaaggc
    11341 acaccgaatg ccagaccgct ttgccggcgt gaaatagtac gcaatcagtg tattggaagg
    11401 acccagagat tcaggttccc cctgatttcg gcgcgaaact gaaagctttt tggggccttt
    11461 tgcggatcag ggtctcggcg ctcgggctcg ttggggtccg gatcttttct gggaggctcg
    11521 gtcttcggcg gatctttgcg cgccggtttc gcccacgtgg ttccttccgg ctggatttgg
    11581 cgttggatca tttgctccct tcctgcccga gtaacagcaa ccctcaggcc ggcgcgcacg
    11641 ccgtgtccgg caatgcgcca tggcccgtcc ggaatccatg cagaacaatg cccttcatgc
    11701 cggctcttgc tgtactacta agatgtaagc tgtgattcgg gcgcccgcac ttgccaaata
    11761 tcggcgaaat cgcccgaagc cggcaagatc cgctattttc cgggagtttt ctcccaaagg
    11821 tcgggcgccg aatgcgaaca ggttgtaaaa attgcctgtg ggggcggggc tttccggggc
    11881 tgaacgtctg gaaacacggg ctttgtcagt ctggcaataa acaggcgaat agagggtgtg
    11941 atcgatctgc ggaccggcgt cgagcacgac cgctcgcgcc aaacaggagg tctaaataca
    12001 atgacccgaa tcaacgcgat acttggggcg ctgctttgtt tgctttacgg gagcttcgtt
    12061 atcccgtgcc aggctgacga atggaacgag aagaccattg tcaccttttc cgagcccgtg
    12121 gaagtgcccg gcgccgtctt gccgccgggc aaatacgtat tcaagctggc tgactcacag
    12181 gcagaccggc acatcgttca gatcatgaat gccgaagaga accatatcta cgcaacagta
    12241 cttgcaatac ctagcgagcg catgcggccc accggcaaga cagagatggc attttacgaa
    12301 atgcccgccg gccagcccaa agctctgaaa acgtggttct atcccggcaa caacatcggc
    12361 caggagttcg cctatccaaa ggcgcgggcc agcgaaattg cgcaagtaac ccacgaaaac
    12421 gttccttccg ctccggagga ggcgccgcag gcgggcgcca cgttacatgg ggggacggag
    12481 gcgagtgcaa acccgagccc cgctcctgct cccgcggaaa ggcccggcgc agctccctcg
    12541 cagccggtag aaacggctca ggcgaatcgg ccggctgaag cgaatccacc ggcggcccag
    12601 gccaatccgc aggccgaaac cacgccgctt tctcgcgctc cggaacggat gcctcgaacc
    12661 gccagcccac ttcctttagt ggcaggcgta ggcatacttc tggccggagc cggcttggtc
    12721 cttcggcggc tcgcaaaagg gatcgcttag gcaatccatc cggtcgagat caacggtgaa
    12781 tgatttggcc cagcgccgga cgaaaactaa agttccccta cgctgggccg cgcgtttttc
    12841 gctgggggcc gggttgttcc tgctggccta ctgcggcttc cagattctgg gcgcggcgct
    12901 ttggcaacgg taccagagct ggaagctgga tcgaagtatt cttaacggca acgcgccgat
    12961 ctactctcgc gcgccgcagt cagcccacac gggaatgcaa cagcggtttc cctaccaggc
    13021 aacggaaggc gcgcccggca ttgaggagca gcgggaaagt tctccggttt cccgccagcc
    13081 ccttgatgca gccgggccgt cgccctcgaa acgcccaggc cgggacgagg ccgcgtctcc
    13141 gggtgtgatc ggacgtctca gcatagcccg gctcgggctc tcggctatga ttcgagatgg
    13201 aaccgatgac gagacgttga gcctcgccgt tggccatcta cccgggagcg cacttcccgg
    13261 agaaccgggc aacgtggttc tcgccgggca ccgtgacacc tttttccgct ctctgcgaaa
    13321 cgcgcagaag aacgacaaaa ttgttatttc cacgccccgc ggagctcatg agtacgtggt
    13381 cgattccatg atgattgtgg atcctcagga aagccaggtg ctgcggccgg ccgtgcagcc
    13441 tacattgacg ctggtgacct gttatccgtt ctcctacatt gggccggctc cgcagcggtt
    13501 tatagtgcaa gccaaagagg tgggcggcgc cggcaatgcg ggactccttc cacaacccag
    13561 cgcggcggcc ggccattcgc cgactccacc acgagcagcg gaggccgcca aaccggccct
    13621 gctgctcgtc cccgaaacgc cgcaggggaa ggggatgcga cgatcgaaat ccgcggccct
    13681 gtctgaaagg cccgctttca cgtatagaga gccggacccg cctcccgcca cctcgcaaaa
    13741 gaggcatggc aaatgggcgc gcgcgttccg cccattcggc aaactggccc gcgccatcgc
    13801 tcatccgctg taacttaaga aaagccgctg gccgccggcg atctgcgcgc cgcgcggcgt
    13861 ttcccacggg ccggttttgc catgaggagg cggtgaaagc ggaaatctcc tattatgtca
    13921 gcgtgtccgc tctcgattct tttcgtgttg ccgccgcctt cggctctgct tgcatggccg
    13981 gcatgatgaa ctccgtggcc ggcggaggca cgctggtcac gttccccacg ctgatctggc
    14041 tcgggctgcc ctccattgcg gccaacgcca caagcacggt tgccatctgg cccggatcgc
    14101 tgggaagcat gtgggtatcg aagggaactg cgggcctcgg aagctcatat ctttcgctgc
    14161 tgttcctcat tcagaagccg gtacaggagc gaatgaagaa aaccggcgga ctggcgcgcc
    14221 actcgccgcg atggatggcc gcggccatgc tgtttcagct attcgtggcg gcccatggcg
    14281 gctatttcgg agcgcatcgg tattttgatg ctggcggccc tgagtgtttt aggtcttaca
    14341 aatattcatc aaatgaatgg cttaaaaaac tttttcgctc tgtgcattaa cggtgtggca
    14401 gcggcgtatt ttgtatccgc caaaatggtt gattggcccg atgcggccgt catggctgcc
    14461 ggagcggtgg caggcggata tggaggcgcg ggagcagccc gccggctggg ccccgcggct
    14521 gtccggcgcg ttgtgattct ggccggcttt ggaatggcgc tatctctttt ttttcgaaaa
    14581 tagctcttcc ggaaaagcta gggccgtgca gaacccgcca ccgttccctt atgctggaaa
    14641 gtgcatgtcg ccatgcgcag tatcttgcag tccttgataa agcgctctac cgattctctg
    14701 gcaatcctgc tcgccggcct gctggttgct tgcgttacgg cgtcgtacct cactcgcgat
    14761 cagggatcga atcgtgctgg ggcgaggcag ccgcccgggc gcggccaagc accgctggtg
    14821 gactaccacc tctggcaaac cgcccgccag ctcgcctcgc aggccgatac cccggaagag
    14881 caggatttcg cccgcgaagc gctgcgcctt gccgatcatg aactggatca ggcttttgcc
    14941 accgagctgc gggaggcggc gctcagcgcg ccgccccgcg gcccgctgaa ggagctggcc
    15001 gatcgtgtga cgcggctgaa atcccgagtg gccgcggggc aggcccggat cgcgcagcta
    15061 acgaaggaag cggccagcaa ggattcggcg atcgatcaac tggaattggc gaaagcgcag
    15121 cttgcgctcg atcaggacga actggaagac gcccagcagg acctggcgcg gcaaggcggg
    15181 gacaggcatt ccaagctgga gagagcgctc cagaaacatg aggcggcgca gcagcagccg
    15241 gcggcgctgc ccaaggtctc cgcttccgcg agccccatta ccctgcaaca gcagttggcg
    15301 ttgtggatgt cactgcgcgc ccgggaacgc cagttgctcg ccgcgcaaca acaagcagca
    15361 gccaaggcct ccgcattgct gcgcgagcat gaggctctgg acaagctggt aaacaacaag
    15421 acaatcccgg cttccgcgga gggatccgcg ccctcaggcg accgggaaga cgaagacacg
    15481 caggctatgc tggcgcgcct gcatcgtctc tcggaccaaa gaaagagcct cagcgagctc
    15541 gacaagcgta ttcaggacgc ccagcagctc ggcgagaatt accggagctg gatcgacgcc
    15601 gtcgagggca ggcggcgcgg cgttctccat ctgctgctga attcagtggc ggcggttctg
    15661 gcgatcctca tcgcctcgtt gttgctttcc aaaggtattc ggcacgcctt cggacgccag
    15721 acggatcgcc gccgcctgcg gcaattgcgc gtcatcgcca gcattgccgt tcaactggcg
    15781 gccgccggcg tgattctgct catcgtcttc ggagcaccga gccagacctc caccataatc
    15841 ggcctggcga cggcggggct gaccgtcgtc ttgaaggatt ttattgtggc gttcttcggg
    15901 tggttcgcgc tgatgggccg gaacggcatc catgtcggcg attgggtgga gatcgagggc
    15961 gtgagcggcg aggtcatcga agtcggacta ttgcggaccg tgcttctcga aatgggcaat
    16021 tgggccagca caggacatcc aaccggccgc cgggtggctt tcgccaacag cttcgccatc
    16081 gaaggccact atttcaattt ctctacagcc ggacagtggc tgtgggagga actgcaattg
    16141 ccgctgcccg ccgccggaga tccccatgaa atagcgcgta agatccaaga gaccatcgag
    16201 cgcgagaccg cagccgacgc ccggctggcg gagcaggact gggagcgggt tacccatcag
    16261 tacgaccttc ggccgttttc ggcaagcccc gccgtcgagc tgcggccgtc cgcctcgggc
    16321 gtcaacatcg tcgtgcgcta cattacgcgc gcgccacagc gttacgagat gaagacgcga
    16381 ctgtatcacg agatcctcga cgtgctccac aaaccggcgc ccagcccagc gagcccggtc
    16441 aaagcctagg cggccaaaag cccctctccc gcctgccgcc ggctgtggct tcaaaactaa
    16501 ggtccgtgga aggtaacccg gcgccggctt ttccggggag ccaggattgt aagaaggagg
    16561 gcgcccgctg ccgctccctg gctcggcgcg gatttgccgc cgacttgtta tcctcataat
    16621 tcggctcttc gattcaccaa actccatgtc tcgaatcatt cgcaaagccg ccgtgctcgg
    16681 cgcaggtaca atgggagcac gcatcgcggc ccacctcacc aacgcgggag tggaaacgct
    16741 gctgctcgat atcgcgccgg accggttgac cgacgcggaa gccgcgcagg gccttacgct
    16801 cgagagcaaa acggtccgca accgcatcgt ccggcagctt ttcgaggcga tgcaaaaatc
    16861 gcggccgccc gcgctcttcc tgccggcgct tgcctcgcgc attcggaccg gaaatttcgc
    16921 agacgatctg ccgaagattg ccggcgttga ctggatcata gaggctatag tggagaactt
    16981 cgacgccaag cgcgccctgc tcgcccaagt ggataactat cgcaagccgg gaacgctggt
    17041 cagctccaac acctcaggac tgccgatcgc gtcgcttgcc gagggcctgt ccgccgattt
    17101 ccgggctcac tggctcgggg tgcatttctt taacccaccg cgccagatgc ggctggtcga
    17161 gatgattccc acgccggaaa ccctccctga agtgatcgat tctctccgcg acttctgcga
    17221 tcgccgcctg ggcaaagtcg tcgttttcgc caaggaccgc cccaatttca tcgccaaccg
    17281 cctctttctg ttcggcctga tggaccttct caagtccatg caaaaacatg ggctcacgat
    17341 tgaagaagtc gatgcgctta ccggaccgct gatcggccgc ccgaatacgg ctacgttccg
    17401 gctggccgac tttgtgggcg tcgatgtgtg tgttttcgtt ggcgagaacc tatatcgcct
    17461 ggtgcccgaa gatgagcagc gcgaggtttt ttcccctccc gaatttctga agcgcatggt
    17521 ggaaaaacgc tggatcggcg acaaagcagg ccagggattt ttcaagcggg agggcaaaga
    17581 acgtctcgtg ctgaaccttg aaacctttga gtacggcccg ccgcggcccg tggatctacc
    17641 ctcgctggaa gccgccaagc aaatccgcga cttgggtgca cggctccggt ttttgttatc
    17701 gcagcaggat cgcgccgggc ggtttctctg ggacacctgg agccggtttc tgctctacgc
    17761 ggccgctcgc attcctgaga ttacggacga cgttcccagc atcgacgcca ccatgcgcta
    17821 tggcttcaat tgggaaaaag gaccgtttga gatatgggac gccatcggag ttcctgaatc
    17881 gacgcagcgc atggaggccg aaggaaaggc aatgccgccg ctggtgcaga agctgctgca
    17941 atcgggcggc gcatcttttt acaaggagct gcaaggagac cgcttttatt tcgatctcgc
    18001 cagcgtctct tacagacccc tttcgcccag gcccggcgtg accagcctgg cagcgctcaa
    18061 acagcgaaac aagatcctgc gctccaaccc cggcgcgagc ctcatcgata ccggcaacgg
    18121 cattttgtgc ctggagtttc attccaaggc caacacgctg gatcgcgatg tcatttccat
    18181 gatccgctca ggagtggaag agacagagtc ccgctacgaa ggactggtca ttggcaatga
    18241 gggccagaac ttttgcgccg gcgcgaatct gcaatggttg ctcgagcttt gcagatcgca
    18301 gcgctgggac gagatcgacc aagccgtccg cgccactcag caggcctttc tagcgctccg
    18361 cgactgccgg aaacccgttg tggccgccgt cttcgggcag acgctcggcg ggggctccga
    18421 gcttgccatg cacacgtcgc gggtccaggc tctggctgaa acgtatatgg gccaggttga
    18481 aaccgccgtc ggcctgattc ctgccgctgg cggcgcaaag gaactgctat ggcgatggat
    18541 ggggacgatt ccggccgacg ccgacccgac tccgtatttg aaagaagtgt tcgaactgat
    18601 cgcaatggcc aaggtctcct cgagcgctgc cgaagcggcc gaatggcgcc tcttgcggtc
    18661 cgaggacccg gtcaccatga accgggaccg cttaatcgaa gacgcccggc agactgcttt
    18721 ggcaatggcc caggccggct atgccgcgcc agccgctccc gcgatgatcc cggtgatggg
    18781 ccgggcgggg ctggcttact tgaaaatgag catatacctt gcccggcggg ccgggtacgc
    18841 cactgagtac gacggccacg tcgcgacgaa gctggcttat gttctcagcg ggggcgatct
    18901 ggcccagccg atgagcgtac cggaaagcta tctcctcgac ctggagcggc agacattcct
    18961 gagtctctgc ggtgaagcga agacacaaga gcgcgtggaa tacatgctcc gcacgggcaa
    19021 gcctttgcgg aattgaggcg gcaggagatc ctcagataga gggcgctcag atgcggcgga
    19081 agactcaaag gggctggaat tactaacccc gagtttttcc aggactccca tggcgatgag
    19141 tcaggagaca gcgcggccgc cgttaccctc ccggccgacc gccgggcttg gaaggccgtc
    19201 aggacccgcc aaatcgccac gccacaagga gggccgccac tacgatcgcc agccagaaat
    19261 attgcaaaac gatctccttg ggccattata ggctcttata tcgctttcca accggcagcc
    19321 gcgcgcgccg cctgtgcgag ttttcgcttc cggagactac agccaccggg tttcggccag
    19381 caaaccggtt tcttgcgcgt ttccctcttg atcggcttgc aaaaatccac tacagtaaca
    19441 gaggcgaata aaaagagaag gagaacgccc ttgcctcttc cccttaccta cccggaaccg
    19501 aagcgcgacc tgagtgaatt cacccggctg gacaatcgcc tcgatgttgc actggcgggt
    19561 tttgagcaac tcgccctgcg agaagactcg ccggtcacgg ccatccggcg gattccggcc
    19621 cggcagcctg attgggcgga gatgccggcg tcgctctccc ctgccctggc tgatgtgctc
    19681 cgcaaacgag gcatagcccg cctctattct caccaggcgg aggcgttcga gcgcattcgg
    19741 gccggggaaa acctggtcat tgtcacgccc actgcgagcg ggaaaacgct ctgctacaac
    19801 ctgccggtgc tgcactacct gatcgagaac ccttccggac gcgccctcta cttgtttcca
    19861 acgaaggccc tggccgagga ccaacttcac gagttccatt ccctagtgga agaaatgggc
    19921 agcgatctcc gggcattcac ctacgacggc gacacgccac aggacgcccg ccgcgccatt
    19981 cgcgagcgtg cgaatgtggt gctcacgaat cccgatatgc tgcactccgg cattctgccc
    20041 caccacacgc gttgggccaa gctgtttgag aacctgcgct atgttgtgat cgacgagctg
    20101 cacacctaca gaggggtgtt cggcagccac gtggcaaatg tactgcgccg gctgaagcgg
    20161 atcgccgctt tctatggttc tcagccgcag ttcatctgct gctccgccac aatcgccaat
    20221 ccccgcgaac tggcgcaggc gctgctggag cagcccgtgc aattgattga ccggaacggg
    20281 gcgacgagcg gcgagaagtt tatcgttttc tacaatccgc ctgtcgtcaa ccggcagtta
    20341 ggcatccgcc gcagctatat ccacgaggcg cgcaatatcg cccgcggctt catcgagcgg
    20401 ggccagcaaa ctcttgtatt cgccaacaat cgcctggcga cggaggttct ggtgacctac
    20461 ctcaaagacg cgtgcgagaa cgggccgggg cgggcggcca gcgtccgcgg ctatcgcggc
    20521 ggctacctgc cgaaagagcg gcgggagatc gagcggggtt tgcgtgaagg caaaatccag
    20581 gcagtagtag cgaccaacgc gctcgagctg ggcatcgata tcggctcgct cgatgtcgtc
    20641 gtcatggcag gctatcccgg cgccatcgct tccacgtggc agcgggccgg gcgcgccggc
    20701 cggcggcagg cgctctcggc ggccgtgctg gtggcctcga gcgctccact cgatcagtac
    20761 atcatcgagc atccggatta cttcttcagc cagtccccag agcacggctg catcaacgcc
    20821 gacaatctgg agatcctggt gaatcacctg aagtgcgcag cgttcgagct gccggtccgc
    20881 agcggcgaaa gcttcggcgg gcgcccaacg gaagatttgt gcaaatttct ggaggagctc
    20941 ggcgtgctgc acttcgccgg caacgcctgg cactggacct cggaatcgta ccccgccgac
    21001 gccatcagcc tgcggaaagt cgccagcgac aactttgtag tgatcgacgt gaccgccgag
    21061 ccgaaagtca tcggcgaagt ggacttttcg tcggcgctca ccacactgca cgaaaaggca
    21121 atttatctgc atgaggcgcg gcaatatcac gtcgagcgcc tgaactacga ggaacgcaag
    21181 gcgtatgtga gaagcgtgga ttgcgattac ttcaccgacg cgatcgacta cacgcacgta
    21241 aaaatcctcg acacctttga atcggagccg ctcggagaca aaccgccaga ggtgaattcc
    21301 ggttcccagg caggcggctt ttcggctccg cctgcggccc gcaaggcgca cggtgaggtc
    21361 cggatcaacc gccagattgt cggcttcaag aagatcaagt tctacacgct cgaaaacgtc
    21421 ggcgcaggcc agctctcaat gcccgagcag gagatgcaca cgacttcctt ctggctgtcg
    21481 ttttcccagc ccttgctgga acgcatgccc gactttacgc cctccgagcg gcagagcggt
    21541 ctggcgggcc tgggcaatgt gctgcgcacc gtcgccgctc tcttgctgat gtgcgatccg
    21601 cgcgatttgg gcgttgcggt cactgaagac ccggcgggca gcgggaattt cgagaggccg
    21661 cagttcgagc ccaatctgta tctctacgac aactaccccg gcggaatcgg ccaaagcgag
    21721 cctctctttc ggatgtgcgc agagctgctg gcaaagagcc gggagttgat cgagggctgc
    21781 ccgtgtaaag caggctgccc gtcgtgcgtc ggccccatcg aggaagtggg agcacgaggc
    21841 agagaagctg ctctgcgata tctggctgag ctgggagcct gatgacctcc gacctcaaat
    21901 cgcagcttgc tctgctgcgg cggcggatca ctcgtatcaa tcgcaagtac cagggcgggc
    21961 ccgcgggcac gagcccgccc ggatctctgg acgggacctt tgctttgtac ggcggcagtg
    22021 atgcgttttc ccccggagat tccgcctgcg agcctgagat ttatgctccg gtgcgctact
    22081 cacctgagaa gtttgttcca ggctccgtgg tcgaaaacga gcaggggcgg tattttctgg
    22141 ctgagaaatt tttcccgtct catcgacatc atggcggcat tgagttttcg cggctgaacg
    22201 agcttgccgg cgagttgctg ccagcgatct ccccggacga tttgccagcg tgcgcccccg
    22261 gacgctgggc cttcctcgat acggaaacga ccggcctgtc ggccggcacg ggcacgtgcg
    22321 catttctcat cggcgtcggg accatcgagc cggcgggctt tcgcgtgcgg ctgtttttca
    22381 tgcgcgacta cgacgaagag gccgccatgc tggccgggct ggcttcgctt ctctcccgct
    22441 tcgaggtgct catcacctat aacggaaaag cctacgacgc gcccctgctc gaatctcgtt
    22501 attgcttgcg ccggatggcc tcgcctctaa gccggctggc ccatctcgat ctgctcttcg
    22561 ccgcccgccg gctctggcgg cttcgcctgg agtcctgccg actgatccag cttgaaaacg
    22621 agattctcgg cgttgagcgg cagggggact tgctggggga gatgattcct taccattatt
    22681 tcgagtactt acggacccgc caggccttcc gcctagcgcc gctgttccat cacaacacga
    22741 tggatatcgt gagtctggcg gctctgacgg ccattgtgct cccggctttc gccgctcccg
    22801 agggctccgg tctgcggcat ggcgaggatc tgctcgggct ggcgcggtgg ctccggcaga
    22861 atggccgatt gccggaggcc gtcaccctct accggcgtgc gatccagcgg ggcctctctg
    22921 accgggacct tttttcggcg ctatgggaaa tggcaattct ggagaagaaa tcgggccatc
    22981 gagaccgcgc ggtggcgatt tggtccgatc tggcattcac ccaaaatgcc ttccgcagcg
    23041 cggcgctcga ggaactggcc aagtactatg agcacacgga acgaaattat tctttggccc
    23101 tggagatgac agccgcagcc ctcgcccttg ccccggcgcc agctttggag aggcggcaga
    23161 ggcggctgaa gcgcaaagcg gccgaacagt cccgcccctt gctccgatag ccgggaaggg
    23221 aagcttcccc gcgcggcggc catggtcaac agcaagaggg cagaagaccc gggtccaggc
    23281 tccgaacggc cgctatttcg ttcgaaatct ggcaattcct ggccctactg cggctggagc
    23341 cgaagcggcc ggcgccccgt ttgagcctgc gttccggccg agggggcggg cttggccgag
    23401 tgcccgcttt cgaggtgggc ttgcgtaccg tggcataatc ggagcagagg ggaatgttgc
    23461 ctagctaaca aacctctggc cgggtcgatt ttttgattgc caaactcgca attctcttcc
    23521 tgctggcggc tccctgggcc tggagctggg gccaggaggg gcatcgcatt accgcgcaaa
    23581 tcgcagaacg gcacttgtct agcaaagcgt tagcggcggt ccgccagatc ctcgggcctg
    23641 gccgccgctt ggaggatatc gccaacattg ccgacgaggt gcggacccgg cgcccggaaa
    23701 ccgcgccctg gcactacgtg gatatcccca ttaccgcaag atcgctggat cgcgggaaat
    23761 attgcccgaa tgatcaatgc attatcggcg ctatcgaacg gttctggcgg gaactggcgg
    23821 ctccgggact ctcaccggag cgccgttacg aggcccttta ttatttcgtg catttcgtgg
    23881 gcgacctgca ccagccactc cactgcggcg accagggcga tcggggcggc aatgacgtcc
    23941 aggtaaaatt ccagggcgaa ctgatgaacc ttcaccaggt ctgggactca ggcattatta
    24001 agtccatgca gctcgacggg gaaaaattgc ggcgtctggg taaaatttcc aggcggcggc
    24061 gccgcgcatg ggcgcgcgga acgcccgagc aatgggccat ggagtcacat gatctcgccc
    24121 gggatgtggc ataccggttc cccgagcccg taaaggacag cgtgatgctg gatcaaaatt
    24181 acgaacggcg ggcggcttcc gtggtgcgca cgcagttggc gaaggcgggt taccgtttgg
    24241 cctatgcgct caatcaaatc taccggtagg cgttgtgaaa cggcaaacgt tccgaaattc
    24301 caaagttgta acacaggaat tagaattgcc ctctggacat ttgtaacttc atggcggata
    24361 cttgaccgaa aggttgctat gcgcacaact ggcgtagggc gataccttgg ggcgctggta
    24421 ctggcagtgg tttggggcgg gctggccccg gcagggaagg cgcagacgga attgaacttt
    24481 tcgcgcatgg gcggcgtggc gcgcattgtc aacaccgata tggccgtact ggaagcgggg
    24541 gacaagcggg acgacttgcc ctgcgaagtt tctcccatga agccggaact gggcttcgat
    24601 ctgcgttttc acaccagcta tcaggtaacc attcccttaa gggaactggc cggaagcggc
    24661 gatcagttgc gggtgttgat tcgggtcacg cccgtagatc agccggcgaa tcaaatttac
    24721 ctcgtagatc gcttcaatgt acctccgatc gacgaggatg ccaagggcga ggcgactttg
    24781 cccggcggct tcaccatggg gccggggaga tacaaggtgg actggctgat gcgcgaccgc
    24841 accgagaggg tctgctccac tcactgggaa gccgaagcca aactggacaa cgacaacaaa
    24901 gacctgccct tgatcatgcc gccgaacacc gtggcgcagc gtccgcagga gcccttcagc
    24961 gagcagccgc ccgtggagcg cgtccagtcg ggccggctgc tgcacgtgaa ggtgctggtc
    25021 aatttctcgc cgacaaatcc gcgcgaagcc agcttgaaac cctgggacat cgaggctatc
    25081 gtctcgatcc tgcgttcaat cgcccgggag ccgcagatcg gccgctttac ggtgattgcc
    25141 ttcaacatgc aggaacagag ggtcatttac cgggaagaca atgtggagcg catcaacttc
    25201 cctgccctgg gagaggccgt ctcgggcatc cacctcggaa cggtggatta ccggcgcctg
    25261 caagacccgc agagcggaac gcgctttctc acttctctgc tgaccgagca gctcggcccg
    25321 cagaatccgg agcccgacgc catcatcatc acgggtccca agctcatgct ggagaagaag
    25381 atcccttccg atgtactcaa ggaagccggc cgcgccaact gcccgatttt ctacctgaac
    25441 tacaacttca atccccgcaa caatccatgg cgggatgcga tcggctcggc gctgaagttc
    25501 tatcggggcc tcgaatactc gatctccctt ccgcacgact tgggctccgc cctaactgat
    25561 atgatgttcc gccttagcaa acgctaggcg aaacaccggt cttgaacttt tcttttccgg
    25621 ggttataatg ccgtaccgtg attctgatgc tcgcagcggc cttgctcctt gcgccgatgg
    25681 cagccggcca gccgaaggaa ccgctcaaat acgagcttgc gggcagagtg cggccgcagg
    25741 ggcgcgcctc cctttcgctg catagcacga cgtctccgtt catgtgctcc tcgcaaagcg
    25801 atccggccgg acgtttccgc ttccgcggcc ttccagcagg aacctacaca ctcgccgttc
    25861 ttcttcccgg ccgcggcgaa gcacggcaga ccatcgaagt cgggcccaag tcggccggcc
    25921 cgaagcggcg cgtagaagtc actgtggact tgaccgatcc caaggtcgtc tcgatcgaag
    25981 cgctgcaacg gcgcgccgtc gtatcggcga aggagctgtc gattccggag cgcgcccgcc
    26041 gggaatatgc cgaggcgcag aagaagttgg ccgaacctga cgtcgccgca gccgttgcgc
    26101 acctcgagaa ggccgttcag atcgctccgc agtactggat cgcttggaac agcctgggaa
    26161 ccatcgccta ccaaagccac gacttctcgc gggccgaaag ttgttttcgc gaagctctca
    26221 aacaaaaccc ggcggctttt gagccgctgg tcaatctggg aggagcgctt ctgacccgcg
    26281 gcaaatggaa cgaagcgctc tcgtacaaca cgcaggccgt gggcgtccgg ccgcatgacg
    26341 ccctggcgaa cgcgcagctt ggaatgaatt tgtactacct agggagcctc gatcgggcat
    26401 ccgagtacct ggcggcggcc aagcgcatcg acccgcgtca tttttcgcat ccgcaactga
    26461 ttttggcgga gatcgatgtg cgccggcatg accggcaggc cgcggcccgc gagcttgaag
    26521 attttcttcg ggtccatcct gactggccgg gagcggaatc catccggcgc aacatcgaca
    26581 ggctgctgat ggcggagtta tccgaatagg cggagatctt gccaagggcg aggtgtatca
    26641 gccagacaca gctctgagga agtggatacc gctcgactcg gccaactttc tcagctctac
    26701 tcgagaaaat cgccagaccc gcattcgtgg cgccgacaga gattttctgg ttgttacggc
    26761 gggctccccg aggctggttt gggtccgccc acagtgaggc gcggccccac cccgttacag
    26821 taagtaggtg cagccatacg gcttcggagt atggcgggtc agcgagcgag gtcgatccgc
    26881 ttcgaggccg cgtcaagacc ggttattcgg aaagagcgga cttgcccgac ttgcaaggct
    26941 tcgcgggcca cgccctgagg gcctggcgtg tcccgcttcc cgagcgcggc ggcgagctgc
    27001 gcgccaaagc tgccggtggc ggcaggaggg gcgtctcccg cccggcaaat gccgatgacg
    27061 ccttcgccca attcgacgcg aaccgcgccg ttctcgatgt gcacgacacg gcccgtcact
    27121 tcgtctccga ccttgtgctc gctgatgtac tcgtcgctcg aatcgggctc gagctgcttc
    27181 atccccagct tgatacgttt cttttcccga tcgaactcga gcaccacggt ccgtacacgc
    27241 tgatcgactt tcagcacttc gttcgcgtga ttcaaacggc ggtcgctggc cagatcagag
    27301 acgtgcagca ggccctcgac gccgtcagcc agctcgacaa aggcgccgaa tttttgcacg
    27361 ttccggacgg ttccttccac caccgtacct ggggggaaac gctttgccgc ttcttcccaa
    27421 ggatcgccga gcgcctgctt caggcccagc gagatgcgct tctccgcggc attgacgccc
    27481 agaaccacgg cctcgaccac atcgcccgtt ttgacgaggt cgcccggacg gcgcacacgc
    27541 ttcgcccatg acatttccga gatgtggacg aggccctcga cgccgggcgc aatctcgaca
    27601 aaagccccga aatccatgac tcgcgtcacc ggcccgcgca cccggtctcc ggggtggagc
    27661 ttctggccga tttcggtcca gggatcgggc gtgagctgct tggttccgag ggcgatgcgg
    27721 ggcttgtcgc ggtcgatgtg aagaatcttc acctcgacct cgtcgcccgg agagaccacg
    27781 gctttgggat cggtgatgcg agaccaggcc atgtccgcca cgtgcagcaa gccatcgacg
    27841 ccgcccagat caacaaaggc gccaaattcc gtcaagctgc gcaccttgcc gcgcaccaca
    27901 gcgccctcgc ggaggctttc gagaaaccgc cggcgggccg ttcgcgcttc ctcttcgagc
    27961 actccgcggc gatccacgat cacattctcg tcctcgatat cgagctggat gatgcggcag
    28021 cgtatttctt cgccgaccag cttttccatc tccgccgctt cccggcagcc gctgcgcgag
    28081 gctggcagga acgcccggac gccgacatcg acgctcaagc cgcctttcac cacgccggtg
    28141 actcggccgg cgatgatttc tctgctctgg aaggcccgtt ccagcgcgct ccagtcctgc
    28201 gggcgccgcg cagccacggc cgagagcagt agatatcctt cggaatcgcg cccgctgatc
    28261 gtgacttgaa tttcgtctcc gacctgaacc ttcagcgcgc catcgggatt ccgcaggtcg
    28321 gcgacgggaa tcacgccttc cgacttgccg ccgatatcca caaacacctg ctcgccggta
    28381 atggccgcga caattcccac gcgcggctca tcgggcccaa acgccccgaa atggcggctg
    28441 ccctcgaagg cttgcaaaag ctcgccaaac gaggactccg gcagattctc gttctgattc
    28501 acggttcaac ccttattgga agttctccga ggagatctct attttgacac agacggagcg
    28561 ggccggcggc gagagattga tcccactttc catgaatgcg ggttcgaagg gactcccggc
    28621 gccggacctt ggccgaaaat cgttccgaaa gccggccgtt taagttgctc cttagcagca
    28681 aacgggaaaa cgacccggcc gcttgcgcgg aacagctttt ggcaggcccc cgtggacgag
    28741 atcgaatgcc aagcgccagc tatcttcggc tctttgcttt cggtttcccc tttgccttcc
    28801 ccggatcctg ctgccgcggc aacggcgttt caccggctcg gcttccattc ccgcactgcc
    28861 ggagaaacgc gcgccggccc agaaactctg cctggcttcc taattcctct tcaatccgca
    28921 atagctgatt gtatttggcg atgcggtcgg tgcggctcaa agagccggtc ttgatctggc
    28981 cggcgttggt ggcaacggcc aaatcggcga taaaggcgtc ctcggtttct cccgaccggt
    29041 gcgagatgac gcaggtgtag ccggcgcggg ccgccagctc catggtcttg agcgtttcgg
    29101 aaagggtgcc gatctggttg accttgatca gaatcgagtt ggcgatcccg cgctcgattc
    29161 ccatttgcag ccgctcggcg ttggtgacga acacgtcgtc gccgacgagc tggattttcg
    29221 agccgagctc ggcggtcagc gcgcgccagc cttcccagtc gtcctcggcc ataccgtctt
    29281 cgatcgagac gataggatac tgccgcaccc agttttcata aaaccgcacc atctggctgc
    29341 tagtccggga gctcttgtcg gattttttga agtgataggc gccgccggca aagaattcgc
    29401 tcgaggccgg gtccaaagcc agggcgattt cttcgcccgg tttgtagccg gcggcgctaa
    29461 tcgcttctag cagaacttcg acggcctctt cgttggattt caaatcgggg gcaaagccgc
    29521 cttcatctcc gaccgcagta gaatggccgc ggcggtggag cacttttttc agcgcgtgaa
    29581 acgtttccgc acccatacgg agcgcatcgg cgaaattacc cgctccgtac ggcacgatca
    29641 tgaattcctg aaaatctacg gaattgtcag cgtgcgcgcc gccgttaatc acgttcatca
    29701 tgggcaccgg caataccctg gcgcctacgc cgcccaggta gcgatacaaa gaggcgccca
    29761 gggcggccgc ggccgcgcgg gcggcggcga gcgacacggc cagtatggca ttggcgccca
    29821 gatgagattt attttttgtc gcgtcgagct cgagcatgat gcggtcgagc gtgaattgat
    29881 cgacggcatc ctggccgttc agagccatat tgagttcgac gttgatgtgg ttgactgctc
    29941 tgcggacccc tttgccgcaa tatcgggacg cgtcgccgtc gcgcaactcc acggcttcgt
    30001 gctcgccggt ggaagcgccg gatggcacag cggcgcgccc gcggctgccg ccggtcagca
    30061 ggacttcggc ctcgacggtc ggattgccgc gcgaatcaag aatctccctt ccaatcactc
    30121 gttcaattct ggtcattagt tccctttccg gtgatgcgct gagatctgaa accggccggt
    30181 catccgatac gctgcggcgc ttcggccatc tgctgcgcca tggtctccct gtcaatcacg
    30241 acgattccgc tgtcggttac gacgaagcct ttgcggcggt cctgctcaag gtcgaacccg
    30301 atttccgacc gttccggaat caccacgccg cgatcgatga tggcgcggcg gatccggctg
    30361 cgccggccga cttgcacatg cggcagcaaa atggagtact cgacttcgga gaagctattg
    30421 acacgcaccc ccggggaaag cacgctgttg atcacgcgcc cgccggagat gatgcatccg
    30481 tgggagacga gcgaatccaa agctacgccc atgcgccgcc cttcgtcggc gaaaacgaac
    30541 ttggccggcg gatattgcgc cagaaaggtt cgaatgggcc aggatgtatc gtacaaattg
    30601 aagacgggac tcaccgagac caggtccata tgggcttcgt agtacgcgtc cagggtgccg
    30661 acatcccgcc agtaccggac ttccttcttg ttctcatcga tgaagtcgta cgcgaaaaca
    30721 cgagcctccc gcgccagcca gggcaggatg tcgcgcccaa aatcgtgcgt agagccggca
    30781 tcctcggcgt cccggcgcag cgcttccgcc agcacctcgg tcgaaaacaa ataaacgccc
    30841 atagaggcgc tgcacatttc gggattgaag cgcgagcgtt ccggagagcc gtgctggggc
    30901 ttttcctcga agccccggat cgacccatct tcggcgattt cggcgatgcc aaaccggtgc
    30961 gcctcggagg gtggaacctg aatcgtagcc agggtgacgt cagctttgtg gtcccgatgc
    31021 caggcagcca gccggccata gttcatcttg tacacatgat ccgccgagag caccagtgtg
    31081 tagggggtgg cctcttccaa gatgctgctg aggttctgat acacggcatc ggccgtaccc
    31141 aagtaccagt tttcgctgac gcgcttcatc ggcggcaaaa cctcgatgaa gtcgccgagt
    31201 tccggagaaa ggatgttcca agcccggcgg agatggcgat tgaggctgag cgacttgtac
    31261 tgcgtcagga tgtagacctt gcgcaggcca gaattaatgc agttggaaag agtgatatcg
    31321 atgatgcggt aggtgccgcc gaaaggaacc gcgggcttgg cgcgctctcg cgttaagggc
    31381 cagagacgtt cgccggctcc tccggccagt agaatcgtga ggacgtcttg catgggaatc
    31441 gggagaaatt cttttataac agatcgccgc ctaggccgct catttgccac aggcgaccgg
    31501 gggcggggta cgcattttga aatgagcctt gccgcggacc gcttggctcg ggtggttttt
    31561 accctcccaa cggggtccta gtgaaattcc taggggggag ttgtaaagat tttgtggaaa
    31621 agcgccttga aataggatac aatctgcttg tcttagaggc ggtcaatttc gaattagtac
    31681 cccgggggga ggtgtagtgg gtggctgaag tcaggctcca agaaggcgaa tctttggaga
    31741 acgcacttcg ccggttcaag cgaaaagtgc agcaggaaga catcatcaaa gaagtgaagc
    31801 ggcactcttt ctacttgaag ccgggcgaaa agaaacgagt aaaacaggct ctggctcgta
    31861 agcgtaaccg caagaaatcg cgtcgagaag ttgagtaaag cgccataacc gaaacccgaa
    31921 tggcatcctc agggcctggg aatagtaaag cagtattagg cttcttacat agaagcaggg
    31981 cggtagcgtt aagcgcaggt gcaagatgcc agagtttgtg gaccgtatcc tgaagtgcac
    32041 ggaatgtggg gcggatttcg tcttcacggc gggcgaacaa cacttctttt acgacaagaa
    32101 cttcaagaat gagcccaagc gttgtaaagc atgcaagagc aaacggactc acggcgggag
    32161 ccatcccggc tcccacaagg tggaaaccgt agccgtttgc tctcagtgtg gtaaacaaac
    32221 cacggtaccc ttcaagccga cacaggggcg gcctgtattc tgccgggagt gtttcctcca
    32281 gcgtcgcggg gtggcggcta cgggaggcgt cagcagcgct tcagcttaga gttccggtct
    32341 tagcctgtaa gccggcctcg ggtgatcctc gcattcaagc gggggcgggt gcattctata
    32401 atggctttcg tggggtcgcc cgccagcttc tttgtccaga acttcgggtg ccgggccgcg
    32461 caagctgacg gcgcagcgct ggaagctgca ctgcaagcca agggtttcgc acccgccggc
    32521 cgcgcggcgg ccgccgacgt cgtcgtcctc aatacctgca cggtaacggc agcggccgat
    32581 gaggatgccc ggcaggctat tcgccgcatc caccgcgaaa acccgcgcgc gcggattctc
    32641 gtgaccggct gctatgccca acgcgctccg ctggaattgg cggcgctgcc gggcgtaagc
    32701 tgggtcgtcg gcaattccca caaacacttg atctccgacc tcgttcccat tcagcccctt
    32761 ttgaatcaac ccggcccggc agatggggaa cccccggcag ctgctccccg gagtgtcaag
    32821 ggcggtcttc ctcaacctgt caaacccctt gacaatctct cggaggagtg cggcccggat
    32881 aatctcgaat ttcacggcca gatcttggta ggcgacatct cgtctcagca ccggttcctg
    32941 cctgtgctcg gctcgggggc gagcgaggcc agcccggcgg tcacgttcga tcgtacccgg
    33001 cccattctga agatccagga cggctgcgac agccgttgct cattttgcat cattccgtcg
    33061 gtccggggcc ggagccgaag cgctccgccg gaactcgtac tcgagcaggc gcgtgtgctt
    33121 gcttcgcagt atcgcgaaat tgtaatcagc ggcatcaacc tgggccggtg ggggcaggac
    33181 ctgcctgggc gaccccgcct ggctggtctc ctgcgtgttc ttctcgagga aaccagcgcc
    33241 gcccgcctgc gcttgagctc ggtcgagccg atggattgga ccgatgagct tctccggctg
    33301 atggccggcg accggcgaat ggcaaggcac attcacgcgc cgctgcaatc cggctccgac
    33361 cgcattctgc gcaagatgca tcgccggtat cgcgcccacc agtacgccga gcgcattgag
    33421 aaggcgtaca cctggatgcc tgaagcggcg ttcggcgccg atgtgatggt cggatttccc
    33481 ggcgaaaccg aagcggattt cgagcaaacc taccgcctga tcgaagagct tccgtttacc
    33541 tacctgcacg tcttcacata ctcgccgcgg ccatccacgc cggcggcgga gctcgccgat
    33601 caggcgccgg cgccggtcaa acgcgaacgc aaccgcgttc tgcgcgaact ggcgcaaaag
    33661 aagggagagg ctttccgccg ccgttgggtg ggcaaacgcc tgtctgcgat caccctcgac
    33721 gattctcacc ccgcgggggc cgtagccttg agcgagaact acatcaaggt gctcatcccg
    33781 ggagtgcggc tgcccggaaa ccagatcgtg gaggttgaag gagcgggcct cgacggcgaa
    33841 tttctcacgg cgcgaaacta acaggacaga gccgcgcaaa ggccgaaccc gtccagcccc
    33901 gatcgcgggc tggcggcctt gtcatcttca atccggtaca gcttcaccgg tcaacccgcg
    33961 tcccgcactc cgagtcagct cgcgacggat cccgactgtg gctccggcca gcgaacattg
    34021 gaatcatcga acgccgcatt tgcctccgag tcacctgttg cacagcctcc gggccggctc
    34081 gtttccacct aaaggaagaa ccctgtccgg ctcgaccata cgtttgacca ggccgcccca
    34141 tatcaaaatg tcaatttcag cccgaactgg atgatccgcg gatctccggc cgcgggattc
    34201 gtccgggtta tgctgccgaa gccccccgcg ccaacctgcg cgaccgggtt atcgaagttc
    34261 gtctggttga agatgttgaa gaactccgcg cggaactgcg tctgaaagcg ctcgttcacc
    34321 cgaaaattct tgaacagccc catgtcccag ctataaagct ggggcccgta cagggtgttt
    34381 cttccaagcg tcccgaaggt cccgatatcg ttgacggcaa atgccgccga attgaaccac
    34441 acacgcttat ccgcacccgc cggcggagta atgccggcgc cggtcagctt cgcccgatca
    34501 ttgcccaggc cggtgagtga attgtctttt ccgctcttga tggtgaacgg cgctccggtc
    34561 tgatactgcc caatgcccgt ccactgccac ccgtttagaa cccatctgcc gaacccgttt
    34621 tgcggtacga cgttgggcaa ctgccaaacc caggagacga cgaaccggtg ccggtggtca
    34681 aaatccgaag gcccgtactg catggcgttc gcatggggga agtaccacgg cattactttg
    34741 tcgccgtaat cgtccaggct cttcgacagc gtgtagttcg ccagcaccgt gaacccccgc
    34801 gaaaaccgct tggtgacgga gagctggaag gagtgatagt acgaccggcg gtcctgcgta
    34861 tagtaatcga cgccgccgat cgtcggcgcg aaaagccgcc tcgcatccgt ccccaaacta
    34921 gacccgggaa tgtacaccgc cgggttgagc tgcacggtgg tccggccgta attggaagcg
    34981 gaaccgacat aggcggcccg caccagccat tcgggaaaga cctcccgctc gagcgtcagg
    35041 ttccagttgt aagccagtgg cgtttcctgg cgcggatcgt aggtcgagat ggcgaccggc
    35101 cggggaaacg gcgcgttggg atcgccgatc gattcgaccc ggacgaggtt gaaatcgctg
    35161 cgcccgcgat acggatcgga gaacggccct tccggttgcg tcacgctgag ccggatgctc
    35221 cacggcggca cgttgaccgc gttgttgttg aagtcccctt cgaggtgctg gtcatagaac
    35281 atgccggcgc cgccgcgcac gctggttttg ccgtcgccaa acacgtccca ggcaaatccc
    35341 agccgtgcgg caacgttgtt gtagtcgccc agcgtgccgt cctccggggc attcggatct
    35401 ccccgaaacg tctctcccgg cgccgcattg acaaacttcg tggacttcac gccgttgaga
    35461 aagtcgggta tccggaagat ttcgattcgc ccccgctcct cgtgccaggg cggggtcggc
    35521 tcatagcgaa cgccgaggtt cagcgtcagc cgccggcgca ccttccagtc gtcctgaaaa
    35581 tagactgccg ggtaggtgtc gcggttggcc ttgtattctc cggtgccctg atcgaagctg
    35641 cggatcactc ccagaaagta gtccgccatg gccagccccg tgctgtcgcc gctgaattgg
    35701 aaatggccgg cccggcggaa ctgattggcg atatccgccc gctggcggtt gatttctccg
    35761 ccgaactgaa tcgtatggct gccgtgcacc cagcttgtgc ggttggccca ttcaaatccc
    35821 gtgcgcggga attttgcctc gaggttgtcg ccgatattga aaaacccgac cgcattgatc
    35881 tcggaaatcg acggcagcgt cggatagatc ggcagccgga ctcccagatc ctgcatactg
    35941 ggcacgccgg agaagtaggg accgcgcgcc gcatggatcc ggttgaagcc gaaatggaag
    36001 tcgttgacca cggccgggct gatcgtccga agccaggtga gcaccgcgct ctggctgcgc
    36061 acgcgcgagg cgagcgtcgg attgcggtac gacagtaggt tggagggatc gtacgtaccg
    36121 gcgttcagga aatgatcgat gaaatagcgg ccgctcagcc ggtctttgcc cgtgagctgg
    36181 tggtccatct tgagaacccc ctgatcctgc tgccagttga tgggccgcgg gattacggtg
    36241 aacccgtctc cgcccaccgc cggaatgtgg gagttcacct tcaccgacga gggatcgaag
    36301 cggctgaccg ggatccggtt gttaggaaag gtcccgccca gaggatcctt cagggcgaca
    36361 ttgcacggct ttccacaggt gctgaagtcg ccgttcctct cgtcgatcgt gggcgcaaaa
    36421 gcggaaagcg aattggccag gttgcggatg cgcgtcccct gccagcccat aaagaagaac
    36481 gttttgttcc ggccgttgta tagtttcggc agcacaaccg ggccgccccc gtaaaccccg
    36541 aattgattcc gcttcaaaaa atcctgggtc ggagaaaacg tgttgcgggc gttcaacacg
    36601 cggttgcgaa caaactcgaa ggcgccgccg tgcaactgat tcgtccccga ccgcgtcacc
    36661 acgttgacca ccgcccctgc attgtttccg gaaaccgcgg agtaattgct ggtttggatg
    36721 gagaactcct gcaaggcgtc cgggaacgga aacgtctggt tttcctggaa atacggatcg
    36781 gtattggttg taccgtccag tttataggcg acctgcgcgg tgcgcgagcc attggaggag
    36841 agttgcaggc cgccaggaat cgatttgccg ctttcgctgc tcaccgagat tactgccgtc
    36901 ccagccacca gagtggagag cttcgccgcg tcgcggccat ttagcggaag ctcgatgatc
    36961 cgcgactggt caactacctc gctcagggtg gaggtagtgg tatccacctg tacggcgctg
    37021 gccgccacac tgaccgtttc agtgactgcg ccgacctcga gagtaatatt caccgtgaga
    37081 ctctgattgg ccagcagctc cacaccggac tggctgaatt tgcgaaagcc agcggactcc
    37141 gacgtaatga cgtactcggt cgggcgcaag ttcggcagga cgtaatgccc gtcggcgccc
    37201 gtgacggcgc tgcggctgaa cccggttcct gtttcggtca ccgtaacggc ggcccctggg
    37261 actaccgctc cgctggaatc gcgcacgctt ccgacgattt ggcctgattg ctgcgccatg
    37321 gcattccctg cgaacgccgc cagcgcggcc acaattgata gaacggactt tcgcatatac
    37381 cctttccttt ccggagaagt ggcgacgcac accgctcctc ccagttctca cccttatgct
    37441 ctgcccttcc ccattaaccc cgttatcttc caatgccggc gctgcccccg cgagccgcct
    37501 ccccaggcga cccggccccg gccggttccc cccgctttcc ccgtctgctt tgttttttcc
    37561 tcctgcgctg tgcgggcggc ccatcagatg gtgcgccggc tgcgacggcg taccatcttc
    37621 gcagtgaaat gcccaccgcc gcggtcagaa gaacaaccgc aacccgatct gaaattggcg
    37681 cggatcgccg cggcccgtgt tgtaatcgcc gcccgccgtg gtaacctgcc cgaaattggc
    37741 gtcgccgaac gtcgtgttgg gattgtcata gtgggccgtg ttggtaaaat tgaacgactc
    37801 caaccgcacc tggcccttca gccgctcggt aatcgagaag tttcggaaca gcgaaaagtc
    37861 gagattcacc acgcccggac cggtcaagat attccggccc acattgccca gcgtccccgg
    37921 agcaggcgcc gagaatttgc tcgtatcgaa ccagagaatc cctttgccca cgttgtgata
    37981 taccgctggc gttccagcgc ttaccaggtt cggccgattg ttcacgaacg gggtgttcag
    38041 cgtggaactg ctgaaggtga tgctcagagg ccggcccgtc tgcgaggtaa agatcccgtt
    38101 cgcctgccag tcgccgaaga tccagcgtcc gatcccggaa tgcaaaaact tcttattcgg
    38161 cccaaacggc aaatcgtaaa tgaagctttg cacccacaca tgcgtcacgt catagtcgga
    38221 acgcccgcgg ttcagggaga caaacatcgg gttgttcagc gagcaattga tatccgtgca
    38281 gaaatcgatc gctttgctga atgtatacga tgtggtcagc atgaatccct gggagaacct
    38341 ccggtcaaat ctcatttgca gcgagttgta gtaactgtga gtgcccaagg ccgtcgtagt
    38401 gctgttggtg cgcttgaaca attggttgag cggctggtaa gcgggcccgc cgccaagcac
    38461 gcggccgtag ttgatctgct cagaggcccg gacgtttacg ccatggttgg caacgtaagc
    38521 gacatccagg gcgaagttgc ccggcagcgc ccgctgaagg gcgatattcc agctctgcac
    38581 atagccgtgg aacaggtcct tgggcacaat gctgtaattc tgattcagcg gcgccgggag
    38641 tatgccgctg gagggtatct gcacaatatc cggcgcgggg atgccggtcg ccagagatcc
    38701 agccgcggca aatgaattcg gcgcggtgaa gttgttcgcc tgtttcacgg gaaagttcat
    38761 aggcccggtc acgcgcggca agaaactgat tccatagccg ccgcgaaaaa ccgtcttgtc
    38821 gttgatgcgg taggccagac cgaatcgggg agcgaagctc agccatttgc catcgatccc
    38881 gaggttgttc ggaatgcttc cgacgcccgc cagctcgagc gtattattcg tggggttgta
    38941 gttggagtag ccggccggaa aatgcggcgt gccggcgggc cagacctccc agcgcaaccc
    39001 gagatccaac gtcagttttt gcgtcacctg ccacttgtcc tgaaaataca ggttgtagag
    39061 aatctctcta cgggccggga agaatacggc gagcccccgg ttgatcttgt tcgcctggtc
    39121 gagcaggaag gccgcaaaag aattgccgaa gctcaatcct ccggaaccgc cgttgagcag
    39181 agttgggcct tccgtgtatg tgaactgccc gcgaggagga tcggtctgtt gcagatcgtt
    39241 tcgctcgcgg cgaatgtcgg cgccccactt gatgacatgg tttcccatgg ttttcgtcca
    39301 gttgttaacg attccatagt tggtcaccga gcgcgcccag ggcaggcttc cgtcgtatcc
    39361 gatcagctcc gggccgaatc cgttcacgac tatctgcgtg aggccgctcg accacgcatc
    39421 caggttgacg cccggaattc cgatatcttt cgaagtagtc aggccgtaat cgacattctt
    39481 cgcatcattg cgatcccgca cgtatcccag ccgcacttcc gtcaccagag tggggctgaa
    39541 aatgtgtgaa tagctcaggc tggggctttg attgcgcgcc gggcctgtgc cggaaaagcc
    39601 ttgattgtga ggtcccccgt agatgccgcc cgggccgtac aatccagggt cgtccacgaa
    39661 ggccacctgg tagctgtaac ggcccgccag gcgatcgttc ggtccgatca cgtagtcaat
    39721 cttaatgtcg aactgatcca gattctttac ccgcaccgtg ttcttttgga aattcgtctg
    39781 gagtccggag gaggtgggcg gcgggataaa gctcaaaatc ttctgcacct gcgagtcgac
    39841 gcggtttaag gggatttgct tgttgggaaa ctccttccgc ccggtaccgt cagcgttacc
    39901 cgtggccgga tcgtaaatcg gcgtcggcga tgccgtcagg tcgccctggc gaaacgccat
    39961 ggtcggaatt gtcgccagat tcgagctgcc gaagtgatca cggctcccct ggtaatcgcc
    40021 aaaaaagaac agcttgtcgc ggatgatccg gccgcctaga gttccgccaa actggttata
    40081 gaccatgcct ggcttggaag tggcgaacgg ctgtgttgcc cggatggccc ggtcctggtg
    40141 aaactcgaac agactgccat gaaattcatt ggtgccggaa cgcatggtga cgttggtcac
    40201 cgcgcctcca gcccgtccaa actccgggtc gtagttgctg gtggaaacgt ccacagtcgc
    40261 gatcgcctcg gccggcaaga cgatggccgt caggttgccc gtgtcaatgt tgttgtcgat
    40321 tccttcaagc tggtaattgt taagctgcct gcccatgccg ttgacgcgag caccgaggct
    40381 gtcctgcgag ttgtagaact ccgaatgcgg gcggaagggc cggcccacgc cgggcaccag
    40441 ggcgatcaga ctttgatagt tccgattgaa tccgagcggc atggtttgca gttgcacttc
    40501 ttcgattttg ccgcccgtgt cggcccggtc ggtctggagc agcggggcct cagcggtgac
    40561 gtccaccgtt tccgatacgg cgccgggact gagttccaga tccacgcgcg ccgtggtgtt
    40621 gggcgtcaga tcaatgccgg agcgaaccat cctacgaaat cccggctgtt ccacctccac
    40681 gcggtaggtt cccggatcca tattggcgaa cacataaaag ccgctatcgt tcgtggtctc
    40741 ggtccggcgc acattggtgt tgatttccgt gaccacgacc tgcgcgcccg gaatgaccgc
    40801 gtgcgagctg tctgtaatgg tgccgaggat tgtggccttc tgcgtttgcg caaaggccgc
    40861 tatcgagagc accgtcaaaa acagaaccgc cggcaaaaca gccgccggat cgaccactct
    40921 ctgagtgccc attttgcccc cttttattaa gcgcgtactg gctggtcttt ccccgcttct
    40981 ctacagattt aaaccctgga actgctcacc ccaaaaatgg ctcggctaca tcaatccgcc
    41041 ccaaccgaaa gggccgtgaa ccgccatcct gtatcgagcc tcaaacccgt ttatttgtgc
    41101 gttagatcga atcgagaaag aaaaaagggc gcaaccccgc cgcggcacgc ctccttgttg
    41161 ggcctctcct tccgaccctg tgctttgatg cggcaattgc cccgttggcc gacaccccaa
    41221 aaccacccga agtcgaaact acgcctcgga tccctatatg tacaggtatt agtactgctt
    41281 gccccaattc cgcttcaccc cgccgaagca ccctgccagc gccagagctt gggaactctc
    41341 tgcaaagcaa gtattcgggc acagtataca tcacgcccat cggcatgcaa gtaagaaaca
    41401 agtaagaaat ttgtggagcg cacaagcggc tgcgttgaag gcgagtttat cgtataaaat
    41461 gcaattccat ctagaatgcc cggggtgcag ggcgtaaaat atgcgctaga acatcggcaa
    41521 aaaaacgggg cacagttccc tgtgccccgc cgtggaagtg atacccgaac cgtttacggt
    41581 atgtagtaga tccgcaaagg aacggtcgtg ctgacaccct tgctgttggt tacggtgagc
    41641 tggaagtcgt actcgccggg atacgggtca ggtccgctca acagcgtcgc tgtcgctcgg
    41701 gcgctgttgg cgttatcgat cctcacgagc ttgctgccgg aggccgtcca gctaaacgta
    41761 agtgagccgc cttcgggatc gtaagaccct gagccatcca acagcaccgt ggcgctctgt
    41821 acgcggcgat cctgcccggg gttggcgacc ggcgctctgc cggttccgcc ggcgccgccg
    41881 ttggcagggt tgacgacgac gatagctgtg acctgcgacc gccggccgta agcaatgagc
    41941 gagtagctgg tggctacggt cgggttcact gtgaccgagc cgttcacggg gaccgaacct
    42001 acgccagtga tgatgacttg cgtggcattc gaagtctgcc agctcaaggt gatcgggtcg
    42061 cccggcttct ggaatgccgg aggattggcg gtgaagttca agattttcac cgcttggatc
    42121 actgtaacca cggctgtagc ggaaacctgg ccttgaccat tgctggcagt caaggtgtag
    42181 gtcgtgtccg tcgtggggct gacagtggaa gttcctgtcg gctgaacgct gccaatgcca
    42241 gtgatggtaa cactcgttgc gttttccact tgccaaacca gagtggcctg gccgcccggg
    42301 agtacggcag ttgggctggc ggaaaattgg agaatacgcg gcacgccggg agcgccgaca
    42361 gttacggtga ccgttgcgtt caccgagccg aagcgattct gggcggtgag cgtgtaggtt
    42421 gtggtttggt ttggagaaac gatggtggag ccgttcttgg ccacattgcc gattccgctg
    42481 atcgaaacct gatcggcgtt ctcggtctgc cagctcaaag tagccgctcc ccccggctgg
    42541 atattagtcg gcgtcgcctg gaaactgacg atccgcgcat ccgggcgtat cacggtaacc
    42601 gtggctgttt gcgtcacttc tccgctggag ttctgggcgg tcagcgtgta gattgtcgta
    42661 gcggtgggag ccaccgagat cgtgccggct ttcgaatcga cctggccgat gccgggggtg
    42721 atagtgaccc ggtcggcgcc ctcgacttgc caaatcaacg tcgatgtctg gccggctgtg
    42781 atttgcggcg ggtcggcagt gaatttcaca atttgcgcct gcgccagatt gcgggtggtc
    42841 acggtgacct tggccgtacc tgtcccgccg tggttgtcag ccacggtcag gcggaagctg
    42901 tattgctttc cagcatcggc ctgaaacgtg gttttggcgg atgtgggatt ggaaagagtc
    42961 accggcggcc caaacgtctg cgtccactgg taggtgatcg ggtcgccatt aggatcataa
    43021 gaaccggagc catcaagggt gatagtcccg ggaggaacgt tgatctgatc cggaccggca
    43081 tcggccacgg gtggatcgtt gccgctgcga acggtccgcg tgagcacctc atagcgcagc
    43141 cgcggaatat ccaccggcac cgggtgcctg acctccgcgt attccctcgg ccgcagccag
    43201 ttcccgagct ggatgccgaa taccacgcgt ccggtgttgt ccttgccggc cagcgtttcg
    43261 ttcaagccgg cctcgaccgt gaaggcccag aaacggttga tgggctgcac gaagcgtagg
    43321 gtgcccccgg gtctgttctc cgcgcctctc tggaacagcg ccccgaggtt cccttccaca
    43381 tacgcgcttc tccacaggcc gacctgggtg ctgaaaccga cttgatcgac gatgcgcaga
    43441 taggtctgct ggatgaggct gttcccgagc ggcgtctcat tgacgacggc attgttcaga
    43501 aatcccttgg tgccgaacgc cccgactcgc ccgcgcccga agacataatc cagggtgagc
    43561 gccccttcgc cgagggttcc tccgctttgg aaattccgga aactgacatg cttgaagcta
    43621 gagaacagtc cggcctggaa gctcttatag cggttcacca agccggcgtc gaactgccct
    43681 tcctggcgat cgcgaaaata caggtattcg ccctcgatct gtaccgcatg tgggctgtca
    43741 ttgctgaaag gagcaaaaaa gcggccctta ccggtgaaag tggtgttgcc gttggtatcg
    43801 ggcccggcat tcaggccgag cagcgagaat ctccgggttc tgcccgcctc gacggcttgc
    43861 ttggcggctt cgctcgcaag attggacagt tcttctttgg agaccggcgg cggaagggca
    43921 ggggcgggcg gcggcgcggg ttgcgccttc aaagcggcca cctgctttct caggtcgtca
    43981 ttctcgttct tgaggtcgcg cagcattgcc atgatgtcgt caagcctttt gagaatatcg
    44041 gagcagcagt ccggggccgg tttcgcgggc ggcgtcatgg cttgaatcgc ctccgccaca
    44101 cctccgtcgc tgacctgttg gccttgcccg ttcgtcacgg tgagaaccac gcgccggttc
    44161 ataaaacgcc cttcgggcgt ccggttgtcc acttccggat tgcgctttcc gcgcccggag
    44221 gtctggatct ggttggctcg cgctccgtac ttcaccagaa agtcacggac cgtgttggcc
    44281 cgggccagag cgagccggtc gttatattgc tccgagccga cgaaatccgt gtggccttcg
    44341 atgtgtaccc ggtagtcggg gtgcgtttgc agcaattccg ccaggcgcaa caggcttgga
    44401 tacccgtcgg agagaatcga tgaattgaac tcaaagttaa tctcttccca gtcgttcttt
    44461 gtcgctcttg tattcaatcc ctgcgcaaga agacaggttg tacaacacaa caggagagcg
    44521 gctatcgctg tatacctttg gaacatgaat tttccccctc tttgacttga aatcatcggc
    44581 atcattacgc cctgaacctt accctacgag tccggcaacc cccggcgtac cggtaagaag
    44641 acttccagac tcgtgcggga atgttgcttg ccgcagccgt gcctcttcac gtactacggt
    44701 tgctaccccg ctttaggcaa tcgtgtttcc acatacattt tcggtttttt tgcccctagg
    44761 aggacactta gtttctcact gtgggtaaaa cttacacacc gagcggggcg gcagcggtgc
    44821 gagtgggcct tttctaccct ctcattcttc gcgagaaaat acgggaagca agacccttgg
    44881 cggtgctttc gggagaccgc tcgcggggaa gaagggcgtc taagaagccg agatcggcgt
    44941 gcctttccgc cgggtgtttg cggccggctc gcggctgagc ggccggcgat gcggacgaac
    45001 cccggtcggc tcacaccgcg tcggaaatgg aggtaaaccg gaaggatcta actttgatcg
    45061 cgggcagcat catgcgcatg ccgccatcgc cgaaactaat gttttcgctt tgcatgaccc
    45121 gctgcggcgt tcccaacagt tcgacttctc tcagcagccg caccaggctc tcgttaaacc
    45181 ggaaatttct gaccgggtgc tggatcttgc cgccctcaat atagaacgtg ccgtcgcgcg
    45241 tcaacccggt caccagggcc tgctgggggt ccaccatacg caaataccac aagcgcgtga
    45301 gcagaatgcc gcgttcggtg gctgcgatca tttccgaaac tggcttgcct tccccatcca
    45361 tgatcaagtt gacgaagaac ggccccggcg gccggccttt ttgcttggcc cagtagcgcg
    45421 tattgctcag attctcgagt accccgtcgc gggccaagta ggccttggca gtgggaaacc
    45481 cgtcgccgct gaaactcgaa gccggaacga cgggatgctg gggatccgtg tagatgttca
    45541 ctcgcgggtc gaagattctc tcgccgatgc gcgtctttcc acccggcgca gaaaacacgc
    45601 tccgcccctc gtccgccggc cgagcgttca gggcaaatag caggctggtc gccatgtcgg
    45661 caagcgcctg cggttcgagg atcgcgggat agtcgcccgg cggcagttcc cgcgctccgc
    45721 gcgagtcgag ggcgcgcttc acggcgatcg ccgcggtttc ccggcaatcc acgctcctca
    45781 tgctgaccga acttgcctcg gcgtagctcg aaccccagcc atcggcagtg cgggcggtga
    45841 tcgaaaacga agccgtgctg gtggaaaagt aagcgaacaa tcccgccgag ttcgccagcg
    45901 cgtgataaac gccgtaattc cgcagaatgc ccgcggcgac gagctttttg ctcctcgcca
    45961 gcgcgaccgc ctcgcccact gccttggccc gggccgcggc cggcatctcg gccgtttcag
    46021 cgtcgtacgc tttcacctcg aggtatttct gcgggccgag cagcggcatg tattccggat
    46081 cttcgggaga gagcgcggcg agagcctccg cttccgcaac cagcgcttgc aaagccgaat
    46141 ccgtgttgcc gccctgcgca ctgagagtcc ccgaggccga ggctttccgc ttgcctttcc
    46201 aggcgatcac ggtgatggct gtcgaatcgg aagttccgct ggtagtggga gaatttcgcg
    46261 caaatcgcaa atgggatctg cgccccgcca ccacgcttac ctggatctcc ggaacggtgg
    46321 cgaacgacaa tatcttctta gtcagttgct gggcttcgtc acgggtcata caaaaactgt
    46381 cttgcaacgg acgcgtgtcg caacggctct ccgccgcgga aagctcgccg gcagccaggc
    46441 gaaaccgctt gcatttgctt gccgctccgg ctgccgattt caaacggcgg ccatcgggcg
    46501 ccacgggcac gggagaaagt cgtcaaaatc tcctcacgat ttctttcctg tgttgatcac
    46561 gtttacctgg cggaaacgcg ccggcgggca gccgtggctt accgggttca actgctccgg
    46621 ctcgcccttg ccgtcgttga acaccccgct caagatgtat tccgtccggc tccccacgcc
    46681 gtcgcaggaa ttccagaaat caggcgtgcg cgcctggtac gccacgtcac gcagcatctc
    46741 gcccttcttc ccgttcttga tctcccagaa tacctgccct ccaaactgaa agttgtaccg
    46801 ctgctggtcg atcgaataac tgcccatgcc caggatcaga atgccgtcct tgatgccagc
    46861 catcagttcc tcctggctca gtttgctatc gagcgggtcg agggaaacgt tcggcatgcg
    46921 ctggaagacg acgctcgacc agctatcggc gtgggcgcag ccatgcgacc gcatgcccgg
    46981 cagcagagcc gcctgctcgc gagtcgtctg gtaatccacg aaaattcctt cgcggatcag
    47041 ataccagcgc tgcgccggaa ctccgtcgtc atcgtatccc accgtggcca ggccgtaagg
    47101 ttgggtccgg tcggcgatca tgtttaccca cttcgagcca tagcgaaatt ttcccagctt
    47161 gtcgggggta agaaaactgg tgcccgcgaa attcgcctcg tagcccaaag cccgatcgag
    47221 ttccgtgggg tgcccgcagc tctcgtgaat cgtgagaaac agattggtcg ggtgcagcag
    47281 cagatcgtac ttgcccggcg ccacgggctt ggcagccagc ttccggaccg cttcttcggc
    47341 ggcctgctcg gcttccgcca ggtgaggata ttcctcgatc cattcgtaac cacgctcggc
    47401 agggtacgcc agtgaactgc gggtcgcaaa ttccccgcgc gccgaatcga ccgccgtcac
    47461 cgtaaacgag cagtcggaac
//
LOCUS       DASREK010000077        12435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2353419, whole genome shotgun sequence.
ACCESSION   DASREK010000077 DASREK010000000
VERSION     DASREK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353419"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1827
                     /locus_tag="VFA54_04160"
     CDS             <1..1827
                     /locus_tag="VFA54_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS50030.1"
                     /translation="TAVVAPNARAAAEYYPANYWYSLIHVPEKSEFPGTGPKGNGISE
                     NMKSQAQWLRLMKTDSCWSCHQLGNKATREIPKNLGQFDSSALAWGRRTMSGQAGGNM
                     TSGLAQLGRERALAMFADWTDRIAAGELPPAPPRPQGVERNVVITEWDWAGPKEYLHD
                     EIATDKRNPTVNANGPLYGSPELSSDYLPVLDPVHYKSSHVKVPVRDPNTTVVSGKVM
                     APSPYWGGEAIWNSKTNVHNPMFDERGRVWLTARIRNEDNPPFCKAGSSHPSAKLFPL
                     AASTRHLAMYDPKTKEFSLIGTCFTTHHLQFAEDANHTLWTSSGGGGGVVGWLNTKLW
                     EETHDEEKAQGWTALILDTNGNGKRDAYVEPNQPVDPTKDKRIAAAFYGVTVSPADGS
                     IWGSVLGFPGSIVRLNPGSNPPETALAEVYEVPWNNPKAPVQGFSPRGLDIDRNGVVW
                     TVLASGHLASFDRRKCQGPLNGPTATGQHCPEGWTLYPEPGPQFKNVADSGSAEASYY
                     DWVDQFNTFGLGANVPIATGNGNDALLALVNGKFVVLRVPYPIGFFAKGLDGRIDDPK
                     AGWKGKGLWSTYATRAPFHMEGGKGTTSKVLHFQLRPDPLAN"
     gene            complement(1857..3962)
                     /gene="recG"
                     /locus_tag="VFA54_04165"
                     /pseudo
     CDS             complement(1857..3962)
                     /gene="recG"
                     /locus_tag="VFA54_04165"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
     gene            complement(4111..5211)
                     /locus_tag="VFA54_04170"
     CDS             complement(4111..5211)
                     /locus_tag="VFA54_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZS50031.1"
                     /translation="MTNATCRAAVLAAFQKPLEMRRYPVPKSIEPGAVLVQVEMAGIC
                     GTDVHLWLGQLPIPIPVILGHETIGRVAALGQGMERDWRGEPLAAGDRITWASSIVCG
                     ECYYCRIKRQLTRCLRRKAYGISYCADEPPHLHGGYAEYIVLRPGTALFRLTDAIATE
                     AVVGAGCALWTAIHGIERMGISWGDVVVVQGAGPVGLASVAVAREAGASRIIVIGGPP
                     HRLERAKRFGADVTLDVDSLPDRRAAVLDVTPGRFGADAVIECVGHPAAVAEGLEYCR
                     DGAKMLVLGQYGDAGATPMNPHLITRKQLQIYGSWGFEPRHVHAALELLGRASTRFPF
                     QEAITHKFPLEGANEALEATRQWRSGKSVLVP"
     gene            complement(5251..6693)
                     /locus_tag="VFA54_04175"
     CDS             complement(5251..6693)
                     /locus_tag="VFA54_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS50032.1"
                     /translation="MFNFSASHEKVPNKSFFLVLAWALALALVSCAPLLGQAAPIQQR
                     ALEYFLDLLKIDTSNPPGRETRVAQYLKGVCDREGIPNELLGGDPQRLNFVARLTAAS
                     SASPAPRPLLLMAHSDVVPVQRSQWTVEPFAALIKDGYIWGRGSQDTKPLLAAELAVM
                     VELKRSGARLRRDVIFLSEADEEAGSSGVQWLIANAWDKISAEFAINEGGFAQRLADG
                     KVLYNVQTAEKIPTRIKLTAHGSAGHGSLPRPDNAVVHLAEAIVRLAHAEQPIRLNAT
                     TREYFRALAKLSEYGKLAPAFGQLEDPSRAPAARLAILRESPMLAAALSTTVSPTMTT
                     AGVKVNVIPSVAEAAVDVRRLPDETKEEILERFRKIINDPQVEIEPLKGDQDNPSTEP
                     SSRTSELYRAIEAVIRAADKNGVVLPNMSLGATDGAFLRARGMGVYGIPLFPTPTEER
                     RAHGNDERLSSASFASGVLLLRDVVRKVAE"
     gene            6692..6859
                     /locus_tag="VFA54_04180"
     CDS             6692..6859
                     /locus_tag="VFA54_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50033.1"
                     /translation="MAKIDGEQDAHRAPEHPGQADAVLYCPVCSTRLAERQCKLICEQ
                     CGYYLSCSDYY"
     gene            complement(6935..8320)
                     /locus_tag="VFA54_04185"
     CDS             complement(6935..8320)
                     /locus_tag="VFA54_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS50034.1"
                     /translation="MPNDQDLDRRDFIARAMGLATAAAVTLPKLRGQSKSPNSTVNYG
                     FIGPGSRGQQLLRHLSKIDTGRCTAMCDIYAENLKKGIATIGSNPEGYYDYRELLARK
                     DIDAVYIAVPLYKHFEITKAAILAGKHVFCEKSLVFTPQEVLELRALAAQHPNQVIQV
                     GLQRRYSEFYQVAKQMIEKGMLGRVTHMHAQWHRNGDWRRPVKDKSLEEQINWRMYTK
                     YSGGLAAELASHQLDVAEWMLGYPFDSVVGVGGLDYWKDGRDIFDNIQLIYTYPKGEK
                     LTYSAITTNAHMPFVGLQGIHEVGEIIMGDAGTIEISLGKGMWYREPDAPKYGRAGTA
                     KENWTAGATVTEAAEGKGFPIIPGQDVDMKQGDSFISREVKYAKRWLYTKGVLVPEEI
                     RDPVAVELEDFLLCVRDGKKPKADLTVGLNDSIGVILSNIAMREERKVKFSEIETLGT
                     AKPAAKKPAKG"
     gene            8547..9104
                     /gene="pyrR"
                     /locus_tag="VFA54_04190"
     CDS             8547..9104
                     /gene="pyrR"
                     /locus_tag="VFA54_04190"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZS50035.1"
                     /translation="MPAIREKAQLMSASEIDRTLVRLAYEVVEKSPDLDKLVFIGVRR
                     RGVPLAQRLSQKIQQIEKRSVPVGTLDINLYRDDLSTVAHQPVVSATEIPFSVTGKDI
                     ILVDDVLYTGRTTRASLDALFDKGRPSRVQLCVLIDRGHRELPIEARFVGKRVETTDS
                     EIIEVKLQEVDGQERVLLVERVSES"
     gene            9109..10032
                     /locus_tag="VFA54_04195"
     CDS             9109..10032
                     /locus_tag="VFA54_04195"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZS50036.1"
                     /translation="MAAGLLGIEELSQEQIQALLDRGRYYQPMQAQSVKKLDTLRGKT
                     IVNLFFEASTRTRTSFEIAAKRLGADTISISAQGSSLSKGESLVDTLNTLDAMRPDAI
                     VMRHSASGAPHFLARFLPTPIINAGDGTHEHPTQALLDAVTILDHRPSLEGLRVAIIG
                     DISHSRVARSNIHLLSKFGVDIVLCGPAPLLPPELVQLAPGITLTTSIEEAVAGADVI
                     MMLRVQLERQHETTFPKGEYFQFYGLRLEHVRLAKPDVLVMHPGPINRGREIASEVAD
                     SPRSVILHQVENGVAVRMAVLEQVASGELRN"
     gene            10118..11398
                     /locus_tag="VFA54_04200"
     CDS             10118..11398
                     /locus_tag="VFA54_04200"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZS50037.1"
                     /translation="MPRLLIRGGRVIDPASSHDAMADVLIEDSRIAAVAPRIEPAGAE
                     ILDAGGLIVAPGFIDMHVHLREPGIEHAETIETGSRAAAAGGFTSVCCMPNTIPVNDS
                     ATVTSFIVERARRSALVNVFPIGAITKDSAGEQLADIGAMKEAGAIAISDDGQPVMNS
                     RVMRRAMEYARSFGLTVIDHCEDLNLSSGGDIHEGAASVRLGLRGIPGCSEDVMVARD
                     ILLSQLTGAKLHVAHISTRNSLAMVRHARQLGIPVTCEATPHHFSLSDADIHDYDSNF
                     KMKPPLRSPCDAGAVADAIAAGVVDAIATDHAPHTGSIKMQEFERCPFGITGLETALG
                     LALEKLVHSGRITLAHMVRLFTAGPAGVLGLQRGTLAPGAAADVTIFSADREWTFDAN
                     QSQSKSRNTPFHQRRFRGGPVVTIVGGRIVWDHR"
     gene            complement(11447..12238)
                     /locus_tag="VFA54_04205"
     CDS             complement(11447..12238)
                     /locus_tag="VFA54_04205"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZS50038.1"
                     /translation="MLFGVVTLLALVLVPFLFWHGTWFGRPLSDREIEQYLNDDSHPR
                     HEQHALVQISQKLDRGDASVQRWYPRVCALAHSREIELRITAAWLMGQDNRADAFHRS
                     LLEALQDPEPLVRRNAALSLARFGDASGRPELRAMLEPFSLLSPADGILRYRLKVRDP
                     AERGTVIAILENGGSRQSEVRSPLPGKILSKTAADGKRVEKGDEIVKLEPSADHVWEA
                     LRALYLVGAPEDLADIEKFTRPLPDWPSRIAEQAKLTAQQIRTRG"
BASE COUNT         2327 a         3928 c         3795 g         2385 t
ORIGIN      
        1 acggccgtgg tagcgcccaa cgcccgggcg gctgcggagt actatccagc caattattgg
       61 tattccctga tccacgtgcc ggaaaaaagc gagtttccag gtacagggcc caaaggcaac
      121 ggcatttccg aaaacatgaa gagccaggcc cagtggcttc ggctgatgaa aaccgatagc
      181 tgttggtcgt gccatcagct cggcaataag gcgacccggg aaatccccaa gaatttgggc
      241 cagtttgatt catcggcgct ggcctggggc cgccgcacca tgtctggcca ggcaggaggg
      301 aacatgacca gcggcctggc gcagctcggc cgcgagcgag ccctggcgat gtttgccgac
      361 tggaccgatc gcattgccgc cggcgagctg ccacccgcgc cgccccgccc gcagggcgtg
      421 gagcgcaatg tcgtcatcac cgagtgggat tgggcggggc ccaaagaata tctccacgat
      481 gaaattgcca cagataagcg gaaccctacg gtcaacgcca atggcccgct ctacggttcg
      541 ccggagctga gctcagatta cctcccggtc ctcgacccgg tgcactacaa gtcgagccat
      601 gtaaaagtgc ctgtccggga cccgaatacc actgtggttt cgggcaaggt catggcgccc
      661 tctccctatt ggggcggcga agcgatctgg aacagcaaga ccaacgtgca caacccgatg
      721 tttgatgaga gagggcgcgt ttggctgaca gcccgaatcc gaaacgagga caatccgccg
      781 ttttgcaaag caggctcgag ccatccttca gcgaaactat ttccgctcgc cgcctctacg
      841 cgccacttgg ccatgtacga tccgaaaacc aaggagttct ccctgatcgg cacctgcttc
      901 accacgcacc atttgcagtt cgccgaggat gcgaaccaca cgctgtggac cagcagcgga
      961 ggaggcggcg gagtagtggg ctggctgaac accaagctgt gggaggaaac tcatgatgag
     1021 gaaaaagccc agggctggac ggcgctcatc ctggatacta acgggaacgg caaacgggac
     1081 gcttatgtag agccgaacca acccgtcgat cccaccaaag acaagcgaat tgcggcggcg
     1141 ttttacgggg tcacggtgag ccccgcggac ggctccattt ggggatcggt gctgggcttc
     1201 ccaggttcga tcgtgcggtt gaaccccggg tccaaccccc cggagaccgc tttggccgaa
     1261 gtctacgaag tgccgtggaa caacccgaaa gcgccggtgc aaggcttctc gccgcgcggc
     1321 ctggacatcg atcgcaatgg cgttgtctgg accgtgctgg caagcggcca cctcgccagc
     1381 ttcgaccggc gcaagtgcca agggcccctc aatggtccca cggcgaccgg acagcattgt
     1441 cccgagggct ggacgctcta tccggagccg gggccgcagt tcaaaaacgt tgccgactcc
     1501 ggcagcgccg aggccagtta ctatgactgg gtcgatcagt tcaacacatt cggcctgggg
     1561 gccaacgtcc cgattgccac cggcaacggg aacgacgccc tgctggcttt ggtgaacggc
     1621 aagttcgtcg tgctccgcgt gccttacccg atcggattct tcgcgaaagg gctggatgga
     1681 cgcattgacg accccaaggc gggatggaaa ggcaagggat tgtggtccac gtacgccacg
     1741 cgagcgccgt ttcatatgga gggcgggaaa ggcacaacca gcaaggttct gcattttcaa
     1801 ctgcggcccg atcccctcgc caactgagcg acagggatcc gggagccgcg gctgcgtcag
     1861 ccgacctgca ccagcccgta gcggcgctgc cagttttccc gaatgtagcc caccagggcg
     1921 cggagatcct cgggggaagg cgggtgttca acatacgcgg cggcttcgga gcgggccagt
     1981 tcgagtacct cgcgatctcg gagcaggtcg gcgacgcgga acgccggcag cccgctctga
     2041 cgcgttccga agaattcgcc gggaccgcgc agccgcaggt cggcctcagc gatccggaag
     2101 ccgtccgttg tctggagcag ggtctggatg cgctcgcgag ccgcctcagt cagcttgcct
     2161 gtgaccagca ggcagtaact ctggtccggc ccgcggccca cgcgtccccg tagttggtgg
     2221 agctgcgcca gcccgaagct ttccgcctgt tcgatcacca tcaccgtggc gttcggcaca
     2281 tctaccccca cttccaccac cgtcgtagcg actagaatgt cgacgcggcc ggctttgaag
     2341 tcctgcatga tggcgtcctt ctccgccggc gccaggcgcc cgtgcagcaa gcgcagccgc
     2401 agattgggaa aaataacgcg ggaaagctgc tcataagatt tttgagccgc tttgatggcg
     2461 cgggtttcgg attcgtcgat cacgggatac acgatgaacg cctggcggcc cgcctggatc
     2521 tgactgcgga ggaagctgta aacctgctcg ctctgatcct gcgtgaaatg gcgcgtctcg
     2581 accgcccggc gtcccggcgg catctcgtcg agaatcgaga catccagatc accgtagacg
     2641 gttagcgcga gggtgcgggg aatcggcgtg gccgtcatca ccagcacgtc cggtgtacgg
     2701 cctttttgga gcagcttcaa gcgttggatc acgccgaagc ggtgctgctc gtcgatgatc
     2761 accaggccca gccgcgcaaa ttccacatcg ccctcgatca gcgcgtgcgt gcccacaacg
     2821 acatgggctg ctccggaagc aatgagcctc ttcagtctgg ctttttctct cgcgttgacc
     2881 gacccggtca gcagaaccgc gacgtgcttc gtcttttcga acatcggacg gcagtacagg
     2941 taatgctgcg ccgccaaaat ctccgtggga gccatgatcg ccacctgaaa accgttggcc
     3001 agcgcgatca tcgcggcttc gagagcgacg atggttttgc cgctgcccac gtcgccttgc
     3061 agcatccggt tcatgggctg cggctgcgac atatcggtgg cgatttcctt caacacgcgt
     3121 ttttgcgccg cggtaggctt gaacgggaga atcgctttga tcttttcccg ggccgcgtcg
     3181 ttcaaagcga aggctatgcc ctccatcgag cgggcgcgcc gccgcttcag ggccaggccg
     3241 cactcaagaa aaaagaactc ctcgaagatc agtcgcattt gcgccggggt ccggaacgca
     3301 ttgaggagcc gcagatccgt acctggggaa gggaagtgcg cctcctgcaa cgcgacagat
     3361 cgaggaggca gccccaaccg gacgcggacg ggttcgggca gcgcgtcgtc cttgacttcc
     3421 acctgttgca aggctcgatg cacgagggtt cgaaaagctc gcgccgtcac cctgccggcc
     3481 gcctcataaa tcgggacgat gcgtcccacg tgcaacgcgg cctcggcttc atcgccatcg
     3541 ccggaaagaa tttcgtactc tgggtgcaag atcgagagtt cgccggaata attgtcgtat
     3601 tcgatttttc cgtaaaccgc tacgcgctgc ccgggcttga gaatgcccgc cagataatcg
     3661 ccccggaacc atttgcaaag cagagtgccg gagccgtcgc gcaccgagac ctggaatatg
     3721 cggacggcgc ttcgccggaa gcggacggcc cccgccgacc ggatctccgc caggacggtg
     3781 gccatttccc ccggcgccag gcgcgcaatc ggcttggtgt tggttcgatc ctcatagcgg
     3841 aagggaacat agtaaagcag gtcctcaatg gtctgaatcc ctttgctttc cagcaaagga
     3901 gcacgggctg gaccaacgcc cttcagatac gtgatcgggg tgttcagctc catcgaaaca
     3961 gcatctgccc ggctctcttc gtcaggctga gcctcgcagt ccggttatcg cccgcggctc
     4021 cgcagagggc gtttcggcag gcgccccagg ccgccggggg aagacagagg atctgattct
     4081 ccaatgggct tcaaatgggc gtactgcgga tcacggcaca agaaccgact ttcccgacct
     4141 ccactgccgt gtagcttcta gggcctcgtt cgccccctcg aggggaaact tgtgtgtgat
     4201 cgcctcctga aatggaaagc gagtggacgc ccgtcccagc agttcgagtg cggcgtggac
     4261 gtggcgcggc tcgaaccccc atgagccgta aatctgcaac tgtttgcgtg tgatcaggtg
     4321 cggattcatc ggcgtcgccc cggcgtctcc gtattggccg agcacgagca tttttgcgcc
     4381 gtcgcggcaa tactccagcc cttctgcgac cgctgccggg tggcccacgc attcgatcac
     4441 cgcatccgcc ccgaaacggc ccggcgtgac atcgaggacc gcagcgcggc ggtccggcag
     4501 ggaatcgacg tcgagcgtga catctgctcc gaagcgttta gcgcgttcca gccggtgcgg
     4561 cggcccgcct atcacgataa tacgcgaagc cccggcttcg cgggcaacgg ccaccgaggc
     4621 caagcccacc ggcccggctc cttgcacgac cactacatcg ccccaggaga ttcccattct
     4681 ctcaatgccg tggatcgcgg tccacagggc gcagccggcc ccgaccacgg cttccgtggc
     4741 aattgcatcg gtaagccgga acagggctgt tccaggacgc agcacgatgt actccgcata
     4801 tccgccgtgc agatgtggcg gctcatcggc gcaataagag ataccgtagg ccttccgcct
     4861 caaacagcgt gtaagctggc gcttgatccg gcagtaatag cactcgccgc acacgatgga
     4921 actcgcccag gtaatgcggt cgcccgcggc cagcggctcg ccgcgccagt cgcgctccat
     4981 accttgaccc agcgccgcca cccggccgat ggtttcatgc ccgaggatca ccggaatcgg
     5041 aatcggcaat tgcccgagcc agaggtgcac gtcggtgccg catatgccgg ccatctccac
     5101 ttggaccagg actgcgcccg gctcaatgct tttcgggaca ggatatcggc gcatctcgag
     5161 cggcttctga aaagcggcca gaacagcggc tcggcaggtt gcgttcgtca tgcggatcgc
     5221 gcttctgctg tcacggctgt gggcaggcgg tcactcagct acctttctca ctacatcccg
     5281 caatagaagc acgccgctcg cgaaacttgc gctcgaaagc cgctcgtcgt tgccgtgggc
     5341 gcggcgctcc tcggtaggtg tggggaacag cgggatgccg taaactccca tgccccgcgc
     5401 ccgaagaaaa gctccgtcgg tcgcacccag ggacatgttc ggcaggacta cgccgttctt
     5461 atcggcggcc cgaatcaccg cttcgatggc ccggtaaagc tcgctggtgc gtgaactcgg
     5521 ctccgtagac ggattatcct gatcgccctt caacggctct atttccacct gcggatcatt
     5581 gatgattttc cggaagcgct ccaaaatttc ctccttggtc tcgtccggca ggcggcgcac
     5641 atcgaccgcc gcctcggcca cgctgggaat cacgttcact ttcactcccg ctgtggtcat
     5701 agtcggcgat accgttgtgg agagtgccgc cgcgagcatc gggctttccc gcagaatcgc
     5761 cagccgggcc gccggcgccc gggaggggtc ttccaactgc ccaaaggcgg gagccagctt
     5821 cccatactcg gatagtttcg ccagtgcacg gaaatattcg cgcgtggttg cattcaggcg
     5881 gattggctgc tcggcgtgcg ccagccgcac gatggcttca gccaaatgga ccaccgcatt
     5941 gtcggggcgg ggcagagagc cgtgccccgc actgccgtgc gccgtcagct ttatgcgcgt
     6001 ggggatcttt tccgccgttt ggacgttata gagcacttta ccgtccgcca gccgctgcgc
     6061 aaaaccacct tcattaatgg caaactcagc gctgatttta tcccaagcat tggcgatcaa
     6121 ccattgcacg ccgctcgatc cagcctcctc gtcggcctcg gaaaggaaaa ttacatcccg
     6181 ccttagtctc gcgccgctgc gcttcaactc aaccatcacg gccagttcgg cagcgaggag
     6241 cggcttggta tcctgggatc ctcttcccca aatgtacccg tcctttatga gcgcggcgaa
     6301 tggctcgacc gtccactggc ttcgctgcac cggcacgacg tcgctgtgcg ccatcagaag
     6361 cagaggccgc ggcgccggag acgcactcga agcggccgtg agacgagcca cgaaattcag
     6421 ccgctgcggg tctccgccga gcagttcatt cgggatgccc tcgcggtcgc agacgccttt
     6481 caggtattgt gcgacgcgcg tttcgcggcc cggcgggttg ctggtgtcga tcttcagcaa
     6541 atcgagaaag tattccagag cccgctgctg gatgggggcg gcttgcccca agagcggggc
     6601 gcaggaaaca agcgccagag ccagcgccca ggctagaaca agaaaaaagc ttttgtttgg
     6661 aactttttca tgggaagctg aaaagttaaa catggcgaaa attgacgggg aacaggacgc
     6721 tcaccgcgcg ccggagcatc cgggccaggc ggatgccgtc ctttactgcc ctgtttgctc
     6781 cacccggctt gccgagcgcc aatgcaagct gatatgcgaa cagtgcggat actatctgag
     6841 ctgttccgac tattactagg cgcccaaaga cgcaaaagcg ccgtgtaaaa tgcaacctcc
     6901 ggaggcgcgc cgttgctgca ttatcctgtt ttttttaccc cttggcgggc ttcttggccg
     6961 ccggcttcgc cgtcccgagg gtttcgatct cgctgaactt caccttgcgc tcttcgcgca
     7021 tggcgatgtt ggagagaatt acgccgatcg agtcgttcaa gcctaccgtc aggtcggctt
     7081 tcggcttttt gccgtcgcgc acacagagca ggaagtcttc cagctccacc gccacaggat
     7141 cccggatctc ctcgggtacc aggacgccct tcgtatacag ccagcgcttg gcgtacttca
     7201 cttcccggct gatgaagctg tcgccttgct tcatatccac gtcctgcccc ggtatgatcg
     7261 ggaaaccttt gccctccgcc gcttccgtta ccgtggcgcc cgcggtccag ttctccttcg
     7321 ctgttccggc ccggccgtat ttcggggcgt ccggctcgcg ataccacata cctttgccga
     7381 gcgagatttc gatcgtgccg gcgtccccca tgatgatttc tcccacttcg tggattccct
     7441 gcaatccaac gaagggcatg tgggcattgg tggtaatggc gctgtaggtg agcttctccc
     7501 ccttaggata cgtgtaaatg agctggatgt tgtcgaaaat atcccggccg tccttccagt
     7561 aatccagccc gccaacgcca accacggaat cgaaggggta gccgagcatc cactcggcca
     7621 cgtcaagctg gtgcgaggca agctcagcag ccaggccgcc tgaatacttg gtatacatgc
     7681 gccagttgat ctgctcttcc aggctcttgt ccttcaccgg ccgccgccag tccccgttgc
     7741 gatgccattg ggcgtgcatg tgggtcaccc tgccgagcat gcctttctcg atcatttgct
     7801 tggcgacctg atagaactcg ctgtagcgcc gctgcaagcc gacctggatc acctggttgg
     7861 gatgctgcgc cgcgagcgcc cggagttcga gaacctcttg cggcgtaaag acgagcgatt
     7921 tttcgcagaa cacgtgcttg cccgccagga ttgccgcttt ggtgatctcg aagtgcttgt
     7981 aaaggggcac tgcgatataa acggcatcga tatccttccg cgccagcaac tcacgataat
     8041 cgtagtagcc ttcggggttg ctgccgatcg tcgctatgcc tttcttgagg ttctcggcat
     8101 agatgtcgca catggccgtg cagcgcccgg tgtcgatctt gctcaaatgc cgcaggagtt
     8161 gctggccgcg gctgccgggg ccgatgaacc cgtagttgac ggtgctgttg ggggacttgc
     8221 tttggcctcg gagttttggg agggtcacgg ccgccgctgt ggccaggccc atagctcgcg
     8281 cgatgaagtc tcttcggtcc agatcttggt catttggcat gtagctcacc ttttgctgtc
     8341 gagtttatca aggcggccgg ctgccgggca atatcttgat tcgaaaagcg atgtggaggc
     8401 cacttctgat tgtcgtcttg cgtttccccc ttccgcgccg ttttccccaa ggcggtcctg
     8461 ccagtccatg acggctggac ggcagcgccc gccggcggcg ggggctgtgc tatcgtagac
     8521 cttacttcca cttagagcgc ccgaacatgc ctgcaatccg cgagaaggcg cagttaatga
     8581 gcgcctcaga aatcgaccgt accctggtga ggctggccta cgaagttgtg gaaaaaagcc
     8641 ccgacctcga taagcttgtt ttcatcggcg tgcggcggcg aggggtgccg ctggcccagc
     8701 ggttgtccca gaaaatacaa caaatcgaga agcgctcggt tccggtcggt acgctggaca
     8761 tcaacctata tcgcgatgat ctatccaccg tcgcgcacca gccggtcgtc agcgccaccg
     8821 agataccttt ttctgtgacc ggcaaggata ttattctggt tgatgatgtc ctctatacgg
     8881 gccgcacgac gcgcgcctct ctggacgcct tgttcgataa agggcgtccc agccgcgtgc
     8941 agctttgcgt gctgattgac cgcggccacc gcgagttgcc gatcgaggcc cgctttgtgg
     9001 gtaaacgcgt ggaaactacc gacagcgaga tcatcgaagt aaagctgcaa gaggtggacg
     9061 gccaggaaag ggtgttactt gtcgagagag tgagcgaatc ctgaagccat ggctgccggt
     9121 cttctgggca tcgaagagct gagccaagag cagatccagg cactgctgga tcgcggccgg
     9181 tactatcagc ccatgcaggc gcagtcagtg aaaaagctcg acacgctgcg gggcaagacg
     9241 atcgtcaatt tgttttttga ggcctcgaca cggacccgca ccagcttcga gatcgccgcc
     9301 aagcgcctcg gcgcggatac catttccatt tccgcgcagg ggtcgagcct ttccaagggc
     9361 gaatccctcg tagatactct caatacctta gacgccatgc gccccgatgc gatcgtaatg
     9421 cgccactcag cctcgggcgc gcctcacttc ctggcccgct ttttgccgac gcccatcatt
     9481 aacgccggcg acggcacgca cgaacacccg acgcaggcgc tgcttgacgc ggttacaata
     9541 ctcgaccacc gccccagtct ggagggcctg cgggtggcga tcatcggcga catctcgcac
     9601 agccgggtgg cgcgctccaa tattcatctg ctctcgaaat tcggcgtgga tatcgtgttg
     9661 tgcgggccgg cgcccctgct gccgccggaa ctggtgcagc tcgcccccgg aatcactctc
     9721 acgacgagca tcgaggaggc ggtcgccggc gccgacgtga tcatgatgct gcgcgtacaa
     9781 ctggagcgcc agcatgaaac gacgttcccg aagggcgaat attttcaatt ttacggcctg
     9841 cggctggagc acgtgcggct ggcgaagccg gatgtattgg tgatgcaccc gggacctatc
     9901 aaccggggcc gcgagattgc ttccgaagtg gccgattctc cgcgctccgt gattttgcac
     9961 caggtggaga acggcgtggc ggtccgcatg gcggtgctgg aacaagtggc gtcgggagag
    10021 ctgagaaatt gaagactcgc gcgcagcgga gcttttccgt ccgattgaga gttgacacgg
    10081 ccgtttccat ccgggagcgc cgcagaggaa ggggaaaatg ccgcgcttgc tgattcgagg
    10141 cgggagagtg atcgacccgg cttccagcca cgatgccatg gccgacgtgt tgatcgagga
    10201 tagccgtatc gcggccgtcg caccgcgcat cgagcctgcg ggcgccgaga ttctcgacgc
    10261 cggcgggctg attgtcgcgc caggcttcat cgacatgcac gtccacctgc gcgagcccgg
    10321 catcgagcac gccgagacaa tcgagaccgg ctcccgcgca gccgccgccg gcggctttac
    10381 ttcggtgtgc tgcatgccga acacaatccc ggtgaacgac agcgctacgg tcaccagctt
    10441 catcgtggag cgcgcccgcc gcagcgccct ggtgaacgtc tttccgatcg gcgccatcac
    10501 aaaagacagc gccggcgagc aactggccga tatcggcgcg atgaaagagg cgggcgctat
    10561 cgccatctcc gatgacggcc agccggtgat gaactcccgt gtgatgcgcc gggcgatgga
    10621 atacgcccgg tccttcggac tgacggtcat tgatcactgc gaggatctca atctcagctc
    10681 gggcggtgat attcacgagg gagcggcctc cgtgaggctc ggcctgcgcg gcattcccgg
    10741 ctgctcggaa gatgtgatgg tggcgcgcga catcctgctg agccagttga ccggggcgaa
    10801 actccatgtc gcccacattt ccacccgcaa ttccctggcg atggtgcggc acgcccgcca
    10861 gttgggaatt ccagtgacgt gcgaggccac gccgcaccac ttctcgctct ccgacgccga
    10921 cattcacgac tacgacagca atttcaaaat gaagccgccg ctgcggagtc cgtgcgacgc
    10981 gggcgctgtg gccgatgcca tcgcggcggg cgtggtggac gccattgcga ccgatcacgc
    11041 cccgcacacc ggcagcatca agatgcagga attcgagcgc tgcccgttcg gcatcactgg
    11101 tctcgagacg gccctcgggc tggcgctcga gaagctcgtg cacagcgggc gcatcacgct
    11161 cgcgcacatg gtgagattgt ttactgccgg cccggccgga gtgctgggcc tacagcgcgg
    11221 cacgctggcg cccggtgcgg cggcggacgt taccattttc agcgccgatc gcgagtggac
    11281 gttcgacgcg aatcagtctc aatcgaaaag cagaaataca cccttccacc agcgccggtt
    11341 tcggggcggt ccggtggtca ctatcgtcgg cggacgcatc gtctgggacc accggtgaag
    11401 cgcgcccaaa actcggtctc cgcagcggcg cgtcccgtac cgtttcctat ccgcgggtgc
    11461 ggatttgctg ggcggtgagt ttcgcctgtt cggcgatgcg agacggccaa tccggcaaag
    11521 gccgcgtgaa tttttcgatg tccgccaggt cctccggagc tcccaccaga taaagagcgc
    11581 ggagggcttc ccaaacgtgg tctgcgctgg gctccagctt gacaatctcg tcgccctttt
    11641 cgacccgttt gccgtcggcc gcggttttgc tcaaaatttt gccgggcagg ggggagcgca
    11701 cttccgattg ccggctgccg ccgttctcca ggattgcgat taccgtgccg cgctccgccg
    11761 gatccctcac cttgaggcga tagcgcaaaa ttccatccgc cggcgacagc agcgagaacg
    11821 gctccagcat ggcccgcaac tccggccggc ccgatgcatc gccgaagcgg gcgagcgaga
    11881 gggcagcatt gcggcggacc agaggttcag gatcctggag cgcttcgagg agggagcggt
    11941 gaaacgcatc cgcccggttg tcctgcccca ttagccaagc ggcggtgatt cgcagctcga
    12001 tctcccggct gtgagccagg gcgcacactc tcgggtacca gcgctgcacg ctggcgtcgc
    12061 cgcggtcgag tttttgggag atttgcacca gtgcgtgctg ctcatgccgc ggatgagaat
    12121 cgtcattgag gtattgctcg atttcgcggt cgctgagcgg cctcccaaac catgtgccat
    12181 gccagaaaag aaacggcacc agtaccagcg ccaacaacgt gaccacgccg aacaacatag
    12241 cccgggggcc gggccgtctt gaagcggttt gattcaatgg cctcccaaga tttcccactt
    12301 aaattgtagc gccagcgaag agcaaaacac ttgccctttg ttttgcctac cggggtgtgc
    12361 ggggcagaac cgtgacagcc tgcggttctg gcggcatgct ttgcctgatc cgctccgtct
    12421 ctgccgggtt taacc
//
LOCUS       DASREK010000078         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2353499, whole genome shotgun sequence.
ACCESSION   DASREK010000078 DASREK010000000
VERSION     DASREK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353499"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VFA54_04210"
     CDS             complement(<1..831)
                     /locus_tag="VFA54_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50039.1"
                     /translation="MKLAFVSPMPPALSGVAEYSAILAASLRKHAQVVVTNRPGPGFD
                     NALYQIGNNRLHLEAYRRALVWPGIAVLHDAVLHHLLLGALDESGYIEEFVYNYGEWR
                     RDLARNLWRSRSRSAADARYFSYPLLRRIVATSRAIVVHNPAAARMAWEAVPGASVTE
                     IPHFFVPPDLPRRWRETRDRLGLASHEFVVGVFGYLRESKRIASVLKAMPAARRGRLL
                     LVGKFVSSELERALAPQLGGSRVIRLERVTDTEFWRLAEITDVCVNLRHPSAGETSGI
                     G"
     gene            complement(828..1490)
                     /locus_tag="VFA54_04215"
     CDS             complement(828..1490)
                     /locus_tag="VFA54_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZS50040.1"
                     /translation="MIAESLARVNERIEKAARRAGRAREQITLIGVSKIMPATAVREA
                     YEAGLRHFGENYVQEFEAKRKELGDLPGAVFHMIGHLQSNKARAAADLFHVIHTVDSA
                     RLARRLNETGKQIQVLIEVKLSPEATKSGIEEQRLPELAECIRSLDKLELRGLMVMPP
                     WPKNPEDSRPYFRRLRSLAEKLRLPELSMGMTGDFEVAIEEGATMIRIGTAIFGPRKA
                     NA"
     gene            1513..2172
                     /gene="tmk"
                     /locus_tag="VFA54_04220"
     CDS             1513..2172
                     /gene="tmk"
                     /locus_tag="VFA54_04220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZS50041.1"
                     /translation="MSAHSGAFFTFEGVDGCGKTTQLARLADRLQAAGYDVVRAQEPG
                     GTRVGREIRRILLDAASTDLRPLPEALLYFASRAQNVEEIILPALAKGSIVLADRFTD
                     ASMAYQGYGRGLGEEIVMALDRLACRGLQPDLTFLIDIDIETSLWRALTRNSGGRPNE
                     SRMERESADFHERVRAGYLEIAARNPSRVRIIDGRKTPDEVADEIWSAAGSFLAARRV
                     R"
     gene            2174..>2804
                     /locus_tag="VFA54_04225"
     CDS             2174..>2804
                     /locus_tag="VFA54_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZS50042.1"
                     /translation="MPANLFDSFLGNSKIVAALRQMLTEDRLPQALLFAGPEGVGKAT
                     LARFLAAAMHCQGPAPRFGAPCGECPVCRRVLAADLSLPEYQKLLEERAKASAEERRE
                     APLVVSTHPDFLTFPPDGPLAQISIEQVRTIREFAQFPPLEGRRRLFLVDRADRIDTA
                     AANSLLKTLEEPPPYLTLVLTAENDFDLLPTIRSRCVPFYFAPLSVEEMN"
BASE COUNT          494 a          887 c          884 g          539 t
ORIGIN      
        1 gccgattccc gaagtttcgc cggcgctcgg gtggcgcaag ttcacgcaga cgtcggtgat
       61 ttcggcgagg cgccagaatt ccgtgtcggt gacgcgttcc aggcggatga cccggctgcc
      121 cccaagctgc ggggcgagcg cccgttccag ctcactggaa acgaattttc ccacgagcaa
      181 caacctgccc cgccgcgctg ccggcatggc cttcaggacc gaggcgatgc gtttggattc
      241 gcggagatag ccgaaaacgc cgaccacaaa ctcatgggat gcaaggccga ggcggtcgcg
      301 cgtctcgcgc catcggcgcg gcagatccgg cgggacaaag aagtgcggaa tctccgtgac
      361 gcttgcgccg ggaacggcct cccaggccat ccgcgcggct gccggattgt gcacgactat
      421 ggcccggctg gttgctacga ttcgccgcag cagaggatag gagaaatagc gcgcgtcggc
      481 ggccgagcgc gaccggctgc gccagagatt tcgcgccaag tcgcggcgcc actctccgta
      541 gttgtacaca aactcttcga tgtagccgga ttcgtcgagc gctccgagca gcagatggtg
      601 cagtacggcg tcgtggagaa cggcaatccc cggccagacc agcgctctcc ggtacgcctc
      661 caggtgcaag cggttgttcc cgatctggta gagggcgttg tcgaagccgg gtcctggacg
      721 gttggtgaca acaacctgcg catgcttccg cagcgatgcg gccagtatgg cggagtactc
      781 cgccacgccg ctcaacgcgg gcggcattgg cgagacgaag gccagcttca cgcgttcgcc
      841 ttccgcggcc cgaagattgc cgtgccgatg cggatcattg tggcgccttc ctcgatcgcc
      901 acctcgaagt ctccggtcat tcccatggaa agctcgggaa gccgcagctt ttcagcaagc
      961 gatcgcagcc ggcggaagta ggggcgcgag tcttccggat ttttcggcca cggcggcatc
     1021 accatcaacc cgcgcagttc gagcttgtcg agactccgga tgcactcggc cagttcgggc
     1081 aggcgctgtt cctcgatgcc gctcttggtt gcctccggcg aaagcttcac ttcgatcaga
     1141 acctggatct gctttcccgt ttcgttgagg cggcgcgcca gccgggcgct atccacagtg
     1201 tgaatcacat ggaacaaatc cgccgccgcg cgcgctttgt tgctttgcag gtgtccgatc
     1261 atatggaaaa cggcgccagg taaatctccg agctccttac gcttggcctc aaattcctgg
     1321 acgtaattct cgccgaaatg acgcaggccg gcctcataag cttcccgaac ggccgtggca
     1381 ggcatgattt tggaaacgcc gatgagcgtg atttgctcgc gcgcccggcc cgcacggcgc
     1441 gccgcctttt caattctttc gttgacccgc gccagagact cggcaatcac aggtttattc
     1501 tatactggtc tgatgtcggc gcattccggc gcgtttttta ccttcgaggg cgtggacggc
     1561 tgcggaaaaa ctacgcagtt ggcgcgcctt gccgaccggc tccaagccgc gggatacgac
     1621 gtggtgcgag cgcaagagcc gggaggtacg cgcgtgggca gggaaatccg gcggattttg
     1681 ctcgacgcgg cctcgacgga cttgcgccct ctcccggaag cgctgttgta tttcgcctcg
     1741 cgcgcgcaga acgtggaaga gatcattttg ccggcgcttg ccaaaggctc cattgttctt
     1801 gccgatcgct ttaccgatgc gtcgatggcc tatcagggct acgggcgcgg cctgggagag
     1861 gaaatcgtca tggccctcga tcgcctggct tgccgcggcc tccagccgga tctcacgttt
     1921 ctcattgata tcgacatcga gaccagcctg tggcgggccc tgactcggaa ctccggcggc
     1981 cgtccgaacg agagccggat ggagcgcgag agcgccgatt tccacgagag agtgcgcgcc
     2041 ggatatctgg aaatcgccgc cagaaatcca agccgggtga ggatcatcga cggccggaaa
     2101 acaccggatg aagtagccga tgaaatctgg tcggccgcgg gatcttttct ggctgcccgg
     2161 agggtgcgtt aacttgccgg cgaacttatt cgattccttt ctcggcaact cgaagattgt
     2221 agccgccctg cggcagatgc tgacggaaga tcgcttgccg caagcgctgc tcttcgcagg
     2281 accggagggc gtgggcaagg ccactctggc gcgctttctg gccgctgcga tgcattgtca
     2341 gggcccggcg ccacgattcg gagcgccctg cggcgagtgc ccggtttgcc ggcgcgttct
     2401 tgccgccgat ctctccctgc cggagtacca gaaattactc gaagagcggg ccaaagcctc
     2461 cgccgaggag cgccgcgaag cgcctctggt agtgagcacg catccggact ttctgacctt
     2521 tcctccggac ggcccactag cccagatttc catcgagcag gtccggacca tcagggaatt
     2581 cgcgcagttc ccgcccctcg aaggccggcg gcggctattc ttggtggatc gggcggaccg
     2641 tatcgatact gcggccgcca attcgctgct caaaacgctc gaggagcccc ccccgtattt
     2701 gaccctggtg ctgacggccg agaacgactt cgatctgctg cccacgattc gctcgcggtg
     2761 tgttcccttc tacttcgcgc cgctcagcgt cgaagagatg aacc
//
LOCUS       DASREK010000079        13880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_375951, whole genome shotgun sequence.
ACCESSION   DASREK010000079 DASREK010000000
VERSION     DASREK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13880
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375951"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFA54_04230"
     CDS             <1..335
                     /locus_tag="VFA54_04230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50043.1"
                     /translation="ISDFANAVFPGGYLPTGAEILQHAAEAGLEIRHLDTLGEHYVLT
                     LRQWSKNFQAARAKIERMKGMQFTRWWELYLALSEAAFRVGRIQLFQTMLTKGHATLP
                     LTRSFASI"
     gene            380..970
                     /locus_tag="VFA54_04235"
     CDS             380..970
                     /locus_tag="VFA54_04235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50044.1"
                     /translation="MPAAFSETAMHWKTAMRSIPLWAALMPLSCLGQKPPPQVMINAP
                     YVTTPAAVIMKMLKLANVTRTDVLYDLGSGDGRIVIAAARSFGARGVGIELNRERIQH
                     AQENARRAGVLPLVTFRRENFFESDLREATVVTLYLLPEVNLALRPKLLRELRPGARI
                     VSNCFDMGDWKPSKQIDVNGCKIYFWSVPAGGTLGN"
     gene            complement(1014..2441)
                     /locus_tag="VFA54_04240"
     CDS             complement(1014..2441)
                     /locus_tag="VFA54_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZS50045.1"
                     /translation="MTDDILYSTIVELSELLKARKISSVELTKAYLDRLEKLGPRYNA
                     LAALTRKIALRQAKDADDDFKRQRIRSPLQGIPYGAKDLLATKGIPTTWGAAPFKNQV
                     FDYDATVIRKLESSGAVLAAKLAMVELAGGGGYRYPSASLFGAGKNPWNPDYWSGGSS
                     SGSAAAVAAAMVPFAIGSETSGSIITPAAYCGVTGLRPTYGIVSRHGAMALSWTCDKL
                     GPLCRSAVDCGLVLHAISGRDWQDPSSSGQRFYYLENYGRPLNELRIGYAPADFHERA
                     AEQARPALQAALDVFRGISPKLSEILLPPMPYADVISTIIQSEAAAIFESLIKSDRLD
                     LMPDEYQKAGLRAALDIPARDYLNAMRVRRLMQENIRLLFRQVDVILSASRAGPASGI
                     NEPLDHPGQPPALSAPPGNGSLIQAGNLAGLPAISLPCGFSTGANLPIAVQLVGKPFD
                     ELTLITLGREFQRRTDWHLKRPPVA"
     gene            complement(2527..2724)
                     /locus_tag="VFA54_04245"
     CDS             complement(2527..2724)
                     /locus_tag="VFA54_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50046.1"
                     /translation="MKKLSRRGFGRLLAASPLLAARAAPQTSSPEDELRAARRRLAEG
                     AQNTAKVSLPMTAEPAFVFRP"
     gene            2858..3574
                     /locus_tag="VFA54_04250"
     CDS             2858..3574
                     /locus_tag="VFA54_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZS50047.1"
                     /translation="MLRTSVVLLYTAVVALAGFLVMIPVTWILGDVRPMYAVAQAVVR
                     SLLKLAGVEVEVGGADLRLLPRPCIYVANHVSNVDPPVLFGYLPRVAIMAKAAVFRIP
                     VLGYALKLGGFIPVERERRDSRRQALEAGMDRLRQGLSLLVFPEGTRSPDGALLPFRP
                     GPFTMAIETQVPIVPITIVGTREVMPKGRSTIRPGRAKIIIHQPVPTTGLDASGRSEL
                     IERVRSIIAAALPADAVRAH"
     gene            3900..4352
                     /locus_tag="VFA54_04255"
     CDS             3900..4352
                     /locus_tag="VFA54_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZS50048.1"
                     /translation="MRRAIDLSIDNVRSGQGGPFAALVVREGKILAEGTNRVTSLNDP
                     TAHAEVVAIREACRILGSFELSGCEIYASCEPCPMCLGAIYWARPARVYFAGTRKDAA
                     EAGFDDDFVYQEIQLPIAERKIPMIPLMREQALEAFEEWKRKPDKVPY"
     gene            4541..4717
                     /locus_tag="VFA54_04260"
     CDS             4541..4717
                     /locus_tag="VFA54_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50049.1"
                     /translation="MRAIRLLLVLLAICACLPAKSTYHYKVTPVASRKNKKIKKHKAK
                     KSKRSRSRSQQRVV"
     gene            4857..5093
                     /locus_tag="VFA54_04265"
     CDS             4857..5093
                     /locus_tag="VFA54_04265"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HZS50050.1"
                     /translation="MKTGDTKARAPRKRSASVTKPGDWTDEQKQQIAEMAYQRFLARG
                     GQHGYAFEDWLQAEAEFAASLASGKPKTARRAKA"
     gene            5252..6178
                     /gene="trxB"
                     /locus_tag="VFA54_04270"
     CDS             5252..6178
                     /gene="trxB"
                     /locus_tag="VFA54_04270"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZS50051.1"
                     /translation="MRDVVIIGSGCAGNTAAVYAARANLNPLVIEGLEAGGQLSLTTL
                     VENFPGFPDGIMGPELVANIKKQAERFGAEYRPGEVTAADLSKRPFRLTVDNQEIVET
                     RTLIIASGASARWLGLPSEQKLIGHGVSSCATCDGFFFRGRPVMVVGGGDSAMEEATF
                     LTKFASEVTITHRRDRFRASKIMLDRARKNEKIKWLTNTVIDEVYDVSKNEVTGVKLH
                     DVRDGRQWEQKIDGLFLAIGHVPNTKIFQGQLELDPDGYIVSKGGARTSVHGVFHAGD
                     VQDRHYRQAVTAAGMGCMAAIEAERLLQAEGH"
     gene            complement(6175..7209)
                     /locus_tag="VFA54_04275"
     CDS             complement(6175..7209)
                     /locus_tag="VFA54_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZS50052.1"
                     /translation="MIQVEPERLELSLLVETWDENRQERRRQAALGSLLTHALLILGL
                     ILLPKIAPPPRTEQPQQNRQHITLLYPPLQELTQTEPNRGPRSKLFLGEAQPPRPPKL
                     FIPKNIPQPANLPGNQQNNRAQSDLAKVEPPQLVPKPELSGAAPLPQILPPSAPPPIP
                     PKLTLENATSPPGGRQGQPQPGILAQERAGNIIEGAVRDLSRSAGRGGTVVGDGYSPG
                     LPDGAVIPSPGNAGSNLELLSDPLGVDFRPYLTRILATVRRNWYAVIPESARLGMVRG
                     RVAIQFIIVRPGIVSKLVIADASGHEALDRAAVAGISASNPFPPLPPEFHGNEIKLQF
                     TFLYNIPVHN"
     gene            complement(7206..8171)
                     /locus_tag="VFA54_04280"
     CDS             complement(7206..8171)
                     /locus_tag="VFA54_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS50053.1"
                     /translation="MTGSKIRVAVIGAGAFGRQHARVLHELPPAELVAVADIDLARAH
                     SVAAPLGVPALADYRELFGRIEAATLAVPTMLHAEIGADLLAHGIDLLVEKPIAADLA
                     SADRLLAAARQHCRILQVGHLERFNPAVEAASAMARLPLFFEVHRMSVFTPRSLDIDV
                     VLDLMIHDIDIVLSLVESKIQQIHAVGLPVLSAQADIAHVRLQFENGCVANLTASRVS
                     TERIRKLRFFQPRQYISVDYSRQDGAIFSVDAANRIGFQPLVVERAEPLAREMAAFLS
                     AVQKRTVPRVTGDAGRNALAVAIKIREEMDAHARVVAATIAGQPV"
     gene            complement(8227..9165)
                     /gene="lpxI"
                     /locus_tag="VFA54_04285"
     CDS             complement(8227..9165)
                     /gene="lpxI"
                     /locus_tag="VFA54_04285"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720208.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HZS50054.1"
                     /translation="MQLAAPRARAVIADPMRLETQHTGRTRVRDLEGGAPLPISMKYA
                     LIAGNGRFPLLVLEAARGAGREMVVVAVKEEAGPEMENLSASLGTRCHWISVGELGRL
                     IEILKAERATHAVMAGQIQHAKIFSSIRPDWRLMKLLLSLKTKNTDALLGAVAGVLAE
                     EGIEVVHSTAFLQPLLAPEGPVTPRNLDEREMADIEYGHRIARALAAFDIGQSVVICD
                     RACVAVEAMEGTDAVIRRAVQYSNGRPLTVVKVSKPNQDMRFDVPVIGPRTIAAMREA
                     NATALAVDAGRTLLLDREELLRQAAEAQIAIAGFPP"
     gene            complement(9173..9949)
                     /gene="lpxA"
                     /locus_tag="VFA54_04290"
     CDS             complement(9173..9949)
                     /gene="lpxA"
                     /locus_tag="VFA54_04290"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HZS50055.1"
                     /translation="MSVHPTAVVHPGAKIDTSADVGPFCIIGEEVEIGPGTRLMAHIY
                     VEGPTWIGRGNIFYPYSTVGVASQDLKYQGERAETRIGDYNRIREFVTIHRGTRGGGA
                     LTSIANSTLIMAYAHVAHDCRVGSHCILANGVTLGGHVTIGDHAVIGAFSGVHQFCRV
                     GQHAMVAGYSVITRDVMPYSLTGVEREARLFGPNKEGLKRRGFDSAAIDRLHKAFRLL
                     GSSKLNTTQALERISAEIEACEEIRELVGFVQSSERGVIK"
     gene            complement(9959..10552)
                     /gene="fabZ"
                     /locus_tag="VFA54_04295"
     CDS             complement(9959..10552)
                     /gene="fabZ"
                     /locus_tag="VFA54_04295"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZS50056.1"
                     /translation="MLTGAMRCVTGAQLALPPFMLDVNQIAQILPHRYPMLMVDRIVE
                     MEPERIVGVKNVTANEAFFAGHFPGFPVMPGVLIVEAMAQVGGVLLLSKIPDRDEKLV
                     LFGSIEQAKFRRPVVPGDQLRIEMRVLKQKASVCKMSGQATVDGAVVAEAVVMCKLAD
                     KNEVESARASAAANSPAGPASGGGDGLPHGATGGPSE"
     gene            10579..11730
                     /locus_tag="VFA54_04300"
     CDS             10579..11730
                     /locus_tag="VFA54_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HZS50057.1"
                     /translation="MRELLSYAVSRRAAQIDLIRRLAEIESPSDNKAAVDRLAEFLAD
                     FLSPVAAIKIYRMRECGNHLRAEFQLPGKSKSGQILALGHMDTVYPLGILAHMPVRIA
                     AGRLWGPGVFDMKAGIAIIIFAAEALRELGRPVTRKLVMQLVSDEETGSICSRDLTER
                     EARKSAAVLVFEPALGSEGRLKTARKGVGDYTIRVRGRPAHAGLDFEAGASATLELAR
                     QLLHIARFTDLRRGITVNPGVIGGGTRVNIVAEAAWAKIDVRVWRMADARRLEKKFRA
                     LRPVDRRTRITVEGGLNRPPFERTPAVRSLYRQARRVAGELGLHLGEAQVGGGSDGNF
                     TAALGVPTLDGLGAVGEGAHAPHESVLIGRLAERVALAAGLIEQIARQP"
     gene            11808..12644
                     /gene="pheA"
                     /locus_tag="VFA54_04305"
     CDS             11808..12644
                     /gene="pheA"
                     /locus_tag="VFA54_04305"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZS50058.1"
                     /translation="MSGASRVAFQGERGAFSEEAAVQLLGRSLVFSPRPRFEDVFRAL
                     AMREVDYAVVPIENTLAGSVHENYDHLLHFKLPIVAETKVRIVHNLIARPGTRFGDIQ
                     RAYSHPVALGQCLRFFEKHPKIKRETYYDTAGSVKMIMEEAPDGAAAIASAAAAEYYG
                     AQILKRSIEDDRQNFTRFFLLARKPPARRSSAKSWKTSIVFTTRNIPGALFRCLSAFA
                     LRDINLAKIESRPLRGKPWEYLFYLDFFGSVQEPRCRRALEHLGELVDLFQVLGCYPQ
                     GA"
     gene            12882..>13880
                     /locus_tag="VFA54_04310"
     CDS             12882..>13880
                     /locus_tag="VFA54_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZS50059.1"
                     /translation="MANDLKPKTTEERDPADPNQLPLLPVRDTVLFPHALLPLTIGRE
                     SSIALVQSLGENKHLGVIAQLDPRVDAPGPQDLHTIGTIAVIHKVIRMPNQNLFIFCE
                     GVSRMRVLEFTALEPFLRGKIEPIREQEPEMTPELEALRQNAISVFQQIVALSPSLSD
                     DLQSIAANIGEIGRLADFMAASLPTLSTAERQEVLNELDVRKRLELVLRKLAKELEVQ
                     QLRSKIYSQVQDQLSQTQREFYLREQMKAIQKELGEGDEQQREIEELRQKIEAAGMPE
                     EVKNEALRELKRLSRMSPAAADYTVTRTYLDWMVVLPWSKSSGVQVDVRKAKEVLDA"
BASE COUNT         2681 a         4118 c         4404 g         2677 t
ORIGIN      
        1 cgatcagcga cttcgcgaac gccgtttttc ccggcggcta tcttcccacc ggggccgaga
       61 tcttgcagca cgccgcggag gccggcctag agatccggca cctggatacg ctcggcgaac
      121 actacgtctt gacgctgcgg cagtggagta aaaattttca ggccgcccgg gctaagatcg
      181 agcgaatgaa gggtatgcag tttacgcggt ggtgggagct atatctggcg ctctcggaag
      241 cggctttccg ggtaggccga attcaattgt ttcaaacgat gctcaccaag ggccacgcca
      301 cgctgccgct gacgcggagc tttgcctcca tttgatgcgc cggtcagcga tgatccgcgg
      361 ctccttctat aatcgtcgaa tgcccgcggc cttttcggaa acggccatgc actggaaaac
      421 cgccatgcgg agcatcccac tatgggcggc gctgatgccg ctgtcttgcc tagggcaaaa
      481 gcccccgccc caggtgatga tcaacgcccc gtatgtaacc acgccggcgg cagtgatcat
      541 gaaaatgctg aagttggcga acgtcaccag gactgacgtg ctctacgacc tgggctcggg
      601 cgacggccgg atcgtgatcg cggcagcgcg gagtttcgga gcccggggcg tcggcatcga
      661 gctgaaccgt gagcgaatcc agcacgcgca ggagaatgcg cggcgggcgg gggtgcttcc
      721 tctggtcaca ttccgccggg agaatttttt cgagagcgac ctgcgcgagg ctaccgtcgt
      781 cactctctat ttgctgcccg aggtgaatct cgcgctgcgc cctaagcttc tgcgcgaact
      841 gaggccggga gcgcgcatcg tttccaattg tttcgacatg ggcgattgga aaccgtccaa
      901 gcagatcgat gtcaatggct gcaaaattta cttttggtct gtgccggccg gaggaactct
      961 cggaaactga gcgctatctc ttcggcctcg gacaagctgc tgcggagtcc gcgtcaggcg
     1021 accggcggcc ttttcagatg ccagtcggta cgccgctgaa actcgcggcc cagcgttatc
     1081 aaagtgagct cgtcgaatgg cttgcccacg agctgcacgg caatgggaag gttcgcgccc
     1141 gtgctgaaac cgcagggcag cgagattgca ggcaagcccg ccaggtttcc agcctggatt
     1201 aggctgccat tgcccggcgg cgcggacagg gcgggcggct gccctgggtg atcgagcggc
     1261 tcgttgatcc cggaagccgg gccagcgcga gaggccgaaa ggatcacgtc cacttgccgg
     1321 aacagcagtc ggatattctc ctgcatcaac cggcgcacgc gcatggcgtt caggtagtcc
     1381 ctggcgggga tgtccagagc ggcgcgaagc cctgctttct ggtactcgtc gggcattaag
     1441 tccagccggt cgctcttaat cagagactcg aaaatagccg ccgcctcgct ctggatgatc
     1501 gtgctgatca cgtccgcgta gggcatcggg ggaagcaaga tctcactcag cttggggctt
     1561 atgccgcgga atacatcgag cgcggcctga agcgcgggcc gggcctgctc ggcggcccgc
     1621 tcatggaaat cggccggcgc atagccgatg cgcagttcat tcaaaggccg gccataattc
     1681 tccaggtaat agaagcgctg cccgctcgag gacgggtcct gccagtcgcg tccggagatt
     1741 gcatgcagca ccaggccgca atccacagcg gagcggcaca gcgggccgag cttatcgcag
     1801 gtccaggata gggccattgc gccatgccgg ctgacaatgc cgtaggtcgg gcggagcccg
     1861 gtgacgccgc agtacgcggc gggcgtgatg atcgatcccg acgtctccga tccaatggcg
     1921 aaaggaacca ttgccgcggc caccgccgcg gccgagccgc tcgacgaacc gccggaccag
     1981 taatcagggt tccaaggatt tttgccggct ccgaagagcg acgcgctagg ataacgatag
     2041 ccgccgcctc ccgccagttc caccatagcg agctttgcgg cgagcacagc gccggagctt
     2101 tccagtttcc ggatcacagt cgcgtcatag tcgaagactt gattcttgaa cggcgccgct
     2161 ccccaggtcg tgggaatgcc ttttgtggcc agcaggtcct tggcgccgta agggatgcct
     2221 tgcaggggac tgcggatgcg ctgccgcttg aaatcgtcgt cggcatcctt cgcctggcgc
     2281 agggcgatct ttcgggtaag cgcagccagg gcattgtacc gcgggccgag cttttccagc
     2341 cgatcaaggt aagctttggt cagctcgacc gaggagatct tgcgggcctt cagcagttcc
     2401 gacagctcga cgatcgtgga gtaaaggatg tcatcggtca tgggtgcttc aacagcatgc
     2461 tcaatctata tttccggctt gctggcggat ccgagcgcga cgggagagag ggagcatagt
     2521 tcgagattac ggccggaata cgaaagcagg ctcggcggtc atgggcaagc tgactttcgc
     2581 ggtattctgc gcgccctcgg cgagccgccg gcgggccgcg cgcagttcgt cctcggggga
     2641 ggaggtttgg ggcgcggctc gtgcggccaa aagcggcgat gccgccagca accttccgaa
     2701 cccacggcgg gaaagctttt tcataaccag cctgcttccc ggctttccgg ccgggtgaag
     2761 ggcgcatacg gaacgcctat cttgcccgga gccgggaagc ctctgcatgc ggaattgcac
     2821 atccacgcgc actataacac aatggcacac gcgacctatg cttcggactt ccgtcgttct
     2881 tttatacacg gcggtggtgg cgctggcggg ttttctggtg atgatcccgg tcacgtggat
     2941 cctgggagac gtgcgcccca tgtacgccgt ggcccaggct gtcgtgagat ccctgttgaa
     3001 acttgccggc gtcgaagtcg aggtcggcgg ggccgatttg agactgctgc cccggccctg
     3061 catctatgtg gcgaaccatg tgagcaacgt ggacccgccg gtgctctttg gctatctgcc
     3121 gcgcgtggcc atcatggcaa aggcggctgt ttttcggatc cctgtgctgg gctatgcgtt
     3181 gaagctcggc ggctttattc cggtggagcg cgagcggcgc gattcgcgcc ggcaggctct
     3241 tgaggcggga atggatcgcc ttcgccaggg gctttcgctg ctggtctttc ccgaaggaac
     3301 ccgcagccct gatggcgcgc tgctgccgtt ccgtccggga ccttttacca tggccatcga
     3361 gactcaggtt ccgattgtgc cgatcacgat tgtcggcacc cgcgaagtga tgccgaaagg
     3421 gcgatcgacg atccggcccg gccgcgccaa aattattatt catcagcccg tccctaccac
     3481 cggcttggac gcctctggcc ggagtgagtt gatcgaacgc gttcgcagca taatcgcggc
     3541 ggcgctcccg gctgacgccg tgcgcgccca ttaagctggc tgtttgcacg cccgcccgac
     3601 accagcgcag agtccttcgt ccatcatcga ggagtctctc tggggtccgg gcggagcgca
     3661 gccggggtag attccaggag ccagttctgt tactggatgc ctctccccga tggtatgctt
     3721 gtgccttcga tggaaggggc ggcaaggcgc cgatcatcag gaatgaagaa tgccgaatag
     3781 agcggcctgg gcggcgctac actgttgccc gcgggcggcg tcgcatctgg cgaatgttca
     3841 cgcgcccttc accctgcgcg agcttgagct aaagttctcg aaacatgatg aaccctttta
     3901 tgcggagggc gatcgatctc tccatcgata acgtgcgcag cgggcagggt gggccgtttg
     3961 ccgcgcttgt tgtccgggag ggtaagatac tggcggaagg cacgaaccgc gtgacttccc
     4021 tcaatgatcc gacggctcac gccgaggtcg tcgcaatccg cgaggcgtgc cgcatcctcg
     4081 gcagtttcga gctttcgggc tgcgaaattt acgccagttg cgagccgtgc ccgatgtgcc
     4141 tcggggcgat ctactgggcg cggccggccc gcgtctattt tgcgggaacc cgcaaggatg
     4201 ccgccgaggc tggcttcgac gacgacttcg tttatcagga aatccaattg cctattgcgg
     4261 agcgcaaaat tccgatgatt ccgctaatgc gcgagcaagc gctcgaggcc tttgaagagt
     4321 ggaagcgcaa gccggacaaa gttccgtact gatcgcacat tttccccgca ggccaggagg
     4381 gcaaaaacgc ttcaccggcg gtcgggaatc cctgctgggg cttgcagacc ccgctgcggg
     4441 ccattttggc gggatggccc ggcagctaat acgatttcaa ttgggaatct aggataatat
     4501 ccggacgtcc ctgcgctgac ggagtgctac aatggggctg atgcgtgcga ttcgactgct
     4561 cctggttctg ctggccatct gcgcgtgttt gccggcgaaa agcacttacc actacaaggt
     4621 gacgccggtc gcctcccgga aaaacaaaaa gatcaagaag cacaaggcca agaagagcaa
     4681 gcgctcccga agccgttccc agcaacgcgt agtttgactg tagctgcgtc ctttgcacta
     4741 cggctgggac tggcattcgc cggttgtgtt ttgacttgcc aagctcacag cggagctcga
     4801 ggctcgctac ggctgtgtca gccggcgagg ggaatagtat aatcaaatct ccggccatga
     4861 aaacgggaga cacaaaagct cgagccccga gaaagcggtc tgcctctgta acgaaaccgg
     4921 gcgattggac ggacgaacaa aagcagcaaa ttgctgaaat ggcctatcag cgctttctgg
     4981 cgcggggagg ccagcacggg tacgctttcg aagattggct ccaggctgag gccgagtttg
     5041 ccgcgtcgct cgcttcgggc aagccgaaaa cggcaaggcg ggccaaagct tgagtgcttc
     5101 ctcgtgcgcg aatcgggcgt ggcgatgtct gagccgccgc cggtggtgtt tcgggcgcat
     5161 cgcagagtga aagtgtagct ttccggagcc cgcctcaatt tcctgcatct catataaaat
     5221 gagaagcttg tcttggaatg gggatttatc catgcgtgac gttgtgatta tcggttcggg
     5281 ctgcgcgggc aacactgccg cggtttacgc cgcgagagcg aatctgaatc cgttggtgat
     5341 tgaaggactg gaagctggag gccagctttc cctcaccacc ttagtcgaaa acttcccggg
     5401 atttcccgac ggcattatgg gtcccgaact tgtggccaat atcaagaagc aggccgagcg
     5461 cttcggcgcg gaataccggc ccggcgaggt cacggcggcc gatttgtcaa aacggccgtt
     5521 tcgcctgacg gttgataacc aggaaatcgt cgaaacgcgg acattaatca tcgcttccgg
     5581 cgcgtccgcc cgctggctgg gcctgccttc ggagcagaaa ttaatcggtc atggcgtgag
     5641 ctcctgcgca acgtgcgacg gatttttctt ccgggggcgt cctgtgatgg tggtgggcgg
     5701 cggcgattcg gccatggaag aggcgacctt cctcaccaag tttgccagcg aggtgaccat
     5761 cacgcaccgc cgcgaccggt ttcgggcctc taagatcatg ctggatcgcg cgcgtaaaaa
     5821 cgaaaagatc aagtggctca ccaacaccgt gatcgacgaa gtctatgacg tcagcaagaa
     5881 tgaagtgacc ggcgtgaagc tgcacgacgt gagagacggc cgccagtggg agcaaaagat
     5941 cgacgggttg tttctggcga tcgggcacgt tccgaatacc aaaatctttc agggccaact
     6001 ggagctcgat ccggacggtt atatcgtttc taagggcgga gcccgtacca gcgtgcatgg
     6061 cgtgttccac gccggcgatg tgcaggaccg gcattaccgc caggcggtga ccgccgcggg
     6121 tatgggctgc atggccgcga tcgaagcgga gaggcttctt caggccgaag ggcattagtt
     6181 gtgtacgggg atgttgtaca ggaaagtaaa ctggagcttg atctcgtttc cgtgaaattc
     6241 cggaggaagg ggcgggaagg gattggaagc gctgatgcct gcgaccgccg cgcggtccag
     6301 cgcttcgtgg cctgaagcgt cggcgataac cagcttagag acgatgccgg gacgaacaat
     6361 gatgaactga atggccacgc ggccgcgtac cattcccagg cgggcgcttt ctggaatcac
     6421 cgcataccag ttgcgccgca ccgtcgccag gatccgcgtg aggtaggggc ggaaatccac
     6481 tcccagcgga tcactgagca actccaaatt gctgcccgca ttgcccgggg atggaattac
     6541 cgcaccatcg ggaagtccgg gactgtatcc gtctccaaca acagttcctc cccggccggc
     6601 gctgcgcgaa agatccctga ccgccccttc aatgatgttt ccagcacgtt cctgggccaa
     6661 aattcccggc tgcggctgcc cctgacggcc accgggaggc gaagtggcgt tttccaaggt
     6721 cagtttcggg ggaatgggag gcggcgcgga gggcggcaga atctgcggca ggggcgcggc
     6781 tccggagagc tccggcttgg gaaccagttg cggaggctcc actttagcca gatcagattg
     6841 cgcccggttg ttctgctgat tgcccggcaa gttggcaggc tgcggtatgt ttttgggaat
     6901 gaagagtttg ggcggccgcg gcggctgtgc ttcccccaag aacagcttgg agcgaggccc
     6961 gcggttgggc tcggtctggg tcagctcttg cagaggggga tacagaagcg taatgtgctg
     7021 ccggttctgc tgcggttgct ctgttcttgg cgggggagca attttgggga gaagaatcag
     7081 tcccaggatc aacagcgcgt gggttagcag ggaccccaag gccgcctgac ggcgccgctc
     7141 ctgacggttt tcgtcccaag tttcgaccag aagactaagt tccagccgct ctggctccac
     7201 ctgtatcaca ctggctgccc cgcgattgtc gccgccacca ctcgggcgtg cgcgtccatc
     7261 tcttccctta ttttaatcgc aacggcaagg gcgtttcgac cagcatcgcc ggttacacgg
     7321 ggaacggtgc gcttctggac cgcagacaaa aacgcagcca tttcccgggc caggggctcc
     7381 gcgcgctcga ccacaagcgg ctggaagccg atcctgtttg ccgcatccac cgaaaagatc
     7441 gcgccgtcct gccgggaata atccactgaa atatattgac gaggctggaa gaaccgaagt
     7501 tttcgtattc tttcggtaga aactcggctg gcggtcaagt tggctacaca gccgttctcg
     7561 aactgaagcc ggacgtgggc gatatcggcc tgggccgaaa ggacaggaag gcccaccgcg
     7621 tggatctgct ggatcttgct ttcgaccaag ctcaaaacga tatcgatatc atggatcatc
     7681 aggtcgagga cgacatcgat gtcaaggctg cggggggtga acacactcat ccgatgcact
     7741 tcaaagaaga gagggagccg ggccatcgcc gaggctgctt cgaccgccgg gttgaaacgc
     7801 tccaggtgtc ccacctgaag aatgcggcag tgctggcgcg ccgccgcgag caaccggtcc
     7861 gcggatgcaa ggtccgcggc aatcggtttc tcgaccagca gatcgattcc gtgggccagc
     7921 agatcggcgc cgatctcagc gtgaagcatt gtcgggacgg ccagtgtcgc cgcctcaatt
     7981 cgcccaaaca actcccggta gtcggcgagc gccggcacac cgagcggagc ggccacagag
     8041 tgagcgcgcg ccaagtcgat gtcggcgaca gccacgagtt cggccggcgg caattcatgc
     8101 aggacgcggg cgtgttgccg gccaaatgcg cctgcgccga tcactgccac gcggattttg
     8161 gagccagtca tgccggcaac cttggaaact ccgcgtgaag cggacgcgat agggagatca
     8221 taggggtcag ggtggaaatc ccgcaatagc gatctgcgcc tcggcggcct gccggaggag
     8281 ctcttcccga tcaagcaaca acgtgcggcc ggcatccacg gccagggccg tagcgttggc
     8341 ctcccgcatg gcggcaatcg tgcgcgggcc aatgaccggc acgtcgaatc gcatatcctg
     8401 attgggtttg gacactttca ccacagtcag gggcctgccg tttgagtatt gaaccgctcg
     8461 ccgtatgacg gcgtcggtac cctccatagc ctctaccgcc acgcaggcgc gatcgcaaat
     8521 aaccacgctc tggccgatgt cgaaggcagc cagagcgcgg gcgatgcgat ggccgtattc
     8581 gatgtccgcc atttccctct cgtcgaggtt tcgcggcgtg acggggcctt ccggcgccag
     8641 caggggttgc agaaacgccg tggagtgcac gacttcaatc ccttcctcgg ccagaacgcc
     8701 ggcgacggcc cccagcagcg cgtcggtgtt cttggttttg agcgacagca gcagcttcat
     8761 cagccgccaa tcgggccgga tcgaggagaa aattttggca tgctggattt gcccggccat
     8821 tactgcgtga gtggccctct ccgccttgag aatctcaatc agacggccaa gctcgcccac
     8881 tgaaatccag tggcaacgtg tcccgagcga tgcgctcaga ttctccattt ccggaccggc
     8941 ttcctccttc accgccacga ctaccatctc ccgcccggcc ccccgagcgg cctcgagcac
     9001 caggagcggg aaccggccgt tgccggcgat caacgcatat ttcatggaaa ttggcagagg
     9061 cgcgccgcct tctagatcgc gcacccgcgt ccggcctgtg tgctgtgttt cgagccgcat
     9121 cggatccgcg atcacagcgc gggccctggg agcagcgagc tgcacgcctg tttcatttaa
     9181 tcacaccgcg ctcggaggac tgaacaaaac ccactagttc gcggatctcc tcgcacgcct
     9241 cgatctccgc gctaatgcgt tcgagggcct gcgtcgtgtt gagtttggaa ctgccaagca
     9301 ggcggaaggc cttgtgcagc cggtcgatgg cggcggaatc gaagccccgg cgtttcagtc
     9361 cttctttatt ggggccaaaa aggcgggcct cgcgctccac gccggtcagc gaatacggca
     9421 tcacgtcgcg ggtgatgacg ctataaccgg caaccattgc gtgctgcccg acgcggcaga
     9481 actggtgtac gccgctgaaa gcgccgatca cggcatggtc cccgatcgtg acgtgccccc
     9541 ccagcgtgac gccattggcc aaaatgcagt gcgagcccac gcggcaatcg tgcgccacgt
     9601 gggcataggc catgatgagg gtggaattcg cgatagacgt gagagctccg ccgcctcgcg
     9661 taccccgatg gatagtcacg aactcgcgga tgcgattgta atcgccgatg cgcgtttcgg
     9721 cccgctcgcc ctggtatttc aaatcctggg atgcgacgcc taccgtactg taaggataaa
     9781 agatatttcc ccgcccgatc caggtagggc cctccacata gatgtgcgcc atcaggcgcg
     9841 tgccagggcc aatctcgact tcttccccga taatgcagaa gggcccgaca tcagcggagg
     9901 tgtcaatttt cgcgccgggg tggactaccg cggtagggtg aacggacatc ggcggcggtt
     9961 actcggaggg gccgccggtt gcgccatgag gcaagccgtc gccgccgccc gatgcaggtc
    10021 ccgcgggaga atttgccgcg gcggacgcac gggcggactc gacttcgttc ttatccgcca
    10081 gcttgcacat tacaaccgct tcggcgacca cggcgccgtc cactgtggcc tgcccgctca
    10141 tcttgcatac tgaggccttc tgcttgagga cccgcatttc gatgcgaagc tggtccccgg
    10201 gaaccacagg ccggcggaat ttggcctgct cgatggatcc gaacagaacg agcttttcat
    10261 cccggtcggg aatcttactc agcagcagca ccccgccgac ttgcgccata gcttccacga
    10321 tcaatactcc gggcatcacc ggaaatcccg gaaaatgccc ggcgaagaaa gcctcgttgg
    10381 cggtgacatt tttgacgccc acgatgcgct cgggctccat ctcgacgatg cggtccacca
    10441 tcagcatcgg atagcggtgc ggcaggatct gtgcaatctg attgacgtcg agcataaacg
    10501 gcgggagcgc gagctgggct ccggtcacgc accgcatggc gcccgtcagc acgcaaaaaa
    10561 ccgctattat agcagaccat gcgcgagctt ctctcttatg ccgtctcccg gcgcgcggcg
    10621 cagattgacc tgatccgccg cctcgcggag atcgaatcgc ccagcgacaa caaagccgcg
    10681 gtcgatcgtc tggccgagtt cctggctgat tttctgtccc cggttgcggc gatcaagatc
    10741 tatcgaatga gagagtgcgg aaatcatttg cgcgctgaat tccagctgcc ggggaagtca
    10801 aaaagcggcc aaattctggc gctgggtcat atggacacgg tttacccctt gggcatcctg
    10861 gcgcacatgc cggtgcgcat cgccgcgggc cggctgtggg ggccgggcgt ttttgacatg
    10921 aaggctggaa tcgcaattat tatttttgcg gcggaagcct tgcgggaact cggccgcccc
    10981 gtcacacgaa aactcgtcat gcagttggtt tccgacgaag agaccggcag catctgctcg
    11041 cgcgatctga ccgaacgcga ggcccgcaag agcgccgctg tgcttgtctt cgagcctgct
    11101 ctcggctcgg aagggagact gaagaccgca cgtaaaggcg tgggcgacta cacgatccga
    11161 gtgcggggca ggccggctca cgccggcctg gattttgaag ccggggcgag tgcaactttg
    11221 gagcttgccc ggcaattgct ccacatcgcg cgattcacgg acctgcggcg cggcattacg
    11281 gtcaatcccg gtgttatcgg aggaggcacg cgggtgaaca ttgtggccga agcagcctgg
    11341 gcgaagattg acgtgcgggt atggcggatg gcggatgccc gccgtctcga aaagaaattt
    11401 cgggcgctgc gccctgtgga ccgccgcacc cggattaccg tcgagggagg tttgaacagg
    11461 ccgcctttcg agcgcacgcc ggcggtccgg tcgctttacc ggcaggcgcg gcgggtcgcc
    11521 ggcgaattgg gcctgcatct cggcgaggcc caggtgggcg gcggctcgga cgggaatttc
    11581 actgctgcgc tcggcgtccc gactctcgac gggctgggcg ccgtcggtga aggggcgcac
    11641 gcgccgcatg agagtgtgtt gatcggccgt ctggccgagc gggtggcgct ggccgccggc
    11701 ctgatcgaac aaattgcgcg tcagccgtag aagctgatac gccgagcccg ccggggttta
    11761 ggccgctggg cgcggcatgg gtctcggcca ggtataatca acgccgtatg agtggagcca
    11821 gtcgagtcgc gtttcaaggg gagcgcggag ccttcagcga agaagccgcg gtacagctcc
    11881 tggggcgtag tctcgtcttc agcccgcgcc cccgctttga ggacgttttt cgcgcgctcg
    11941 ccatgcggga ggtcgattac gcggtcgtgc ccatcgagaa caccctcgcc gggtcggttc
    12001 acgaaaacta cgatcacttg ctccatttca agctgccgat agtggcggaa acgaaagtcc
    12061 gcatcgtgca taacctgatc gcgcgcccgg ggactcggtt cggtgatatc cagcgggctt
    12121 attctcatcc ggtggcgttg ggacagtgcc tccgtttctt tgagaaacat cctaagataa
    12181 aacgtgaaac gtactacgat acggcgggca gcgtaaagat gatcatggaa gaggcgcccg
    12241 atggagccgc cgccatcgcc tccgctgcgg ctgccgaata ctacggcgct cagatcctga
    12301 aacgctcgat cgaggacgat cgccagaact ttacccgatt tttcctgctg gcgcgcaagc
    12361 cccctgccag gcgcagcagc gcgaagagtt ggaaaacgtc gatcgttttc acgacccgca
    12421 atattcccgg cgccctgttc cggtgcctca gcgcttttgc cctgcgcgat atcaatctgg
    12481 ccaagatcga atcccgccct ttgcggggaa aaccttggga atatcttttc tatctcgact
    12541 ttttcggatc ggtgcaggag ccgcggtgcc ggcgcgcgct ggagcatttg ggcgaactgg
    12601 tggacttatt tcaagtgctc ggctgctatc cgcagggagc gtagcgttca ggctcgcgga
    12661 agagttccgg gcgaacgccc acgtgccggg ggtggttacg catttataca gagtggttag
    12721 gcgttataca gaaaggcgct gcctggcctg cttgtccggt taaccttcca cggattggaa
    12781 gatgtccgta tgcagagcga cgttgggaaa tgtccggcgg agcataaagg agcggcgcgg
    12841 ttttcaccca caaggggctt cctatggcat cctaaaggca tatggccaat gacttaaagc
    12901 caaaaaccac cgaggaacgg gacccggcgg accccaatca gcttccgctg ctgcccgtgc
    12961 gcgatacggt cttgttccct catgcactgc tgccgctgac catcggccgt gaaagctcaa
    13021 tcgcgctggt ccagtcgctt ggcgagaaca agcacctggg cgtgatcgcc cagctcgacc
    13081 ctcgtgtcga tgcgccaggc ccgcaggacc tgcatacaat cggcactatc gccgtcattc
    13141 acaaagtcat tcgcatgccc aaccagaacc tgttcatctt ctgcgagggc gtttcgcgga
    13201 tgagagtgct ggaattcact gccctggagc cctttctacg agggaagatt gaacctatcc
    13261 gcgagcagga acccgagatg acgccggaat tggaagcgct tcggcagaat gcgatcagcg
    13321 tttttcagca gatcgtggcg ctttctccgt ctctttccga cgatttgcaa tccatcgccg
    13381 cgaacatcgg cgagatcggc cggctggcgg atttcatggc ggcttccctg ccgacccttt
    13441 ccaccgccga gcgccaggag gtgctgaatg aactggacgt gcgcaaacgc ctcgagctgg
    13501 tgttgcgcaa gctggccaaa gagctggaag tgcaacagct acgttcgaaa atttattccc
    13561 aggtacagga tcagctttct cagacccaga gagaatttta tctgcgcgag cagatgaaag
    13621 cgattcaaaa agagctggga gaaggggacg agcaacagag ggaaatcgaa gagctccggc
    13681 agaaaatcga agcggccggc atgccggagg aagtgaaaaa cgaagcgcta cgggaattga
    13741 agcgcctttc gcggatgtcg cccgcggcgg ccgattacac tgttacgcgc acctatctgg
    13801 actggatggt cgttttgccc tggagcaagt cgtccggcgt ccaggtggat gtccggaaag
    13861 cgaaggaggt cctcgacgcc
//
LOCUS       DASREK010000080         6635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1861408, whole genome shotgun sequence.
ACCESSION   DASREK010000080 DASREK010000000
VERSION     DASREK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861408"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VFA54_04315"
     CDS             <1..1064
                     /locus_tag="VFA54_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS50060.1"
                     /translation="MESIPDRIYFKDSSCRYTHLNHAQARLLGISDPESARGKTVCDF
                     SSEPWAREALLDEEKIVRTRSPLIDKVETVRRPNGEVRWFSTTKAPILDAAGRVTGIV
                     GVSRDISERRRAVESLARQADELARYNAELERFAFVSAHDLQEPLRTMASFAQLLAIR
                     YRGKLGADADQYIEMIISGAARMRALLEDLLTYSRLARRQPGLEPVHTERVFQRALAR
                     LEGQIKRAGAIVTCDPLPVVQADPARLELVFQNLLGNAVKFRAGARPRIHVSAARNGT
                     EWIFSVRDNGIGIEAQHFTRIFLIFERLHSKQEYAGSGVGLAICKRIVEGHRGRIWVE
                     SEPGKGSTFYFTIPILEEA"
     gene            1175..3469
                     /locus_tag="VFA54_04320"
     CDS             1175..3469
                     /locus_tag="VFA54_04320"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HZS50061.1"
                     /translation="MHTIESTPELVEEVYRNMERNLAVVRKRLGRPLSLAEKILFSHL
                     DDPEGQDLTAGVSYLMLRPDRVAMQDATAQMALLQFGLAGREHVAVPTTVHCDHLIQA
                     RAGAKTDTQFAIVENNEVYEFLRQASAKYGIGFWKPGSGIIHQVVLENYAFPGGMMIG
                     TDSHTPNAGGLGMFAAGVGGADAVDVMAGFPWEVKNPKLIGVRLTGELSGWTAPKDVI
                     LYLCGVLTVKGGTNRVIEYFGPGARSISCTGKATITNMGAELGATTSIFPYDEHMAAY
                     LRATRRAALADLAGRRRELLEADPEVEQHPEKYFERVVEIDLSRLEPYMVGPHTPDLA
                     RPVSRLAADVKEHGYPDRISVALIGSCTNSSYEDISRAADVAEQARAHGARMKAPFLV
                     TPGSEQVRSTIERDGQMAALESIGATVLANACGPCIGQWKRQEISQGEPNTIVTSFNR
                     NFPKRNDGNPATHAFIGSPEIVIAYGLAGRLSFDPLRDEIETPDGKRFRLQPPQKAPE
                     LPPNGFVSTVEGFVPPPADGSSVKVEIAPGSTRLQALEPFPAWDGKDFREMPILVKAK
                     GKCTTDHISPAGPWLRFRGHLDNISDNLFSGAINAFTGQAGQGINVLTGERNVPFAKI
                     ARDYKAKGLRWVAVGDENYGEGSSREHAAMSPRFLGAAAIIVRSFARIHESNLKKQGV
                     LPLTFADPSVYDRVQENDRISLIGLKDLAPGRPVKGILHHADGSSESFDLNHTLNREQ
                     MEWFKAGSALNLLRLQAQPEPVAG"
     gene            complement(3534..5021)
                     /locus_tag="VFA54_04325"
     CDS             complement(3534..5021)
                     /locus_tag="VFA54_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS50062.1"
                     /translation="MDQTLRILMLEDVPADAALIERELRKAKFRFAAKRVETRDDFIR
                     ELQTFAPDLILSDYSLPSFDGFSAMALAKELAPLTPFIIVTGSLDEETAVECIKNGAA
                     DYVIKERAVRLGPAIESALEKKRAREAKELAEERLHRSEETFRGIVEAAAEGIWITNR
                     EGKTTFVNQTMADMLGYSLQETMNRRIWSFVSEEDRESFRSKLLSRSRTGAERYDVRF
                     VRKDGAELWAIVSASPMFDNNGQFAGSLKMVTDITERKRSEKELAAKAEQLARYNAEL
                     ERFAYVSAHDLQEPLRTVASFTQLLAQRYQGKLDQDGNRFIQLAVAGCARMRQLIEDL
                     LAYSRVARRPAEVALTDCQAVCTRAIENLRAVIDESAAVVTYGPLPAILADPARLGQL
                     FSNLIGNALKFRGQEPPRVHISADRKGSEWLFAVRDNGIGIDPAHAEKIFEIFQRLHG
                     KQQYSGTGVGLALCKMIVQSHGGRIWVESQPGKGATFYFTLPARS"
     gene            complement(5031..5465)
                     /locus_tag="VFA54_04330"
     CDS             complement(5031..5465)
                     /locus_tag="VFA54_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50063.1"
                     /translation="MDDGEPLEVLLVEDNLDDVALTLRALRKHNLINEVHVVNDGAAA
                     LEHIFGRGDGRDLHRPRVIFLDLKLPKVSGLEVLSKLKADERTRTIPVVVLTSSHEDQ
                     DVTECYRLGVNSYIVKPVEFDDFIRSVSELGFYWMLLNKPAV"
     gene            complement(5458..>6635)
                     /locus_tag="VFA54_04335"
     CDS             complement(5458..>6635)
                     /locus_tag="VFA54_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS50064.1"
                     /translation="SPASDDKNRRADIAREMRLSDVPHRLRLAATLAGWLWLLGAAPP
                     AGVMSNCPAAIIQMGGPGIPAQKPREGIINLNLSGKKWTFHLTLGFLSVAGILLLLLL
                     LAAAILFALMARRRAKESEAANRNLEREILDRKRAEEEVRRLNAELERRVAERTEQLH
                     YAMKQLEAFSYSVSHDLKAPLRAISGYSKALLEDYGVQLEDEARGYLESIARGAGRMG
                     QLINDLLAFSKLGRKEMALVEVNMSELARSVFEEIQRAAPERKLECSFGDLPPARGDR
                     AMLHQVWANLLSNAAKFTRHKENAAIEIGGSSNGSRQNTYYVKDNGAGFDMQYVNKLF
                     GVFQRLHSSEEFEGTGVGLAIVERVVQRHGGRVWAEGKVNEGAAFYFTLPAEKTSHG"
BASE COUNT         1253 a         2040 c         2058 g         1284 t
ORIGIN      
        1 tgatggagag cattcccgac cgcatctatt tcaaggattc ttcctgccgc tacacccacc
       61 tcaatcatgc ccaggcgcgg ctgctgggaa tcagcgatcc ggagtcggcc aggggaaaga
      121 ccgtctgcga tttttcttcc gaaccgtggg cgcgcgaagc gctgctcgac gaggaaaaaa
      181 tcgtaaggac ccgctcgccc cttattgaca aggtcgagac ggtccgccgc cccaatggag
      241 aagtgcggtg gttttcgact accaaggcgc ccattctgga cgcagccggc cgggtgacgg
      301 ggatcgtcgg cgtgtcgcgg gacatcagcg agcggcgaag ggccgttgaa tcgcttgcca
      361 gacaggcgga tgagctagcg cggtataacg ccgagcttga gcgctttgct ttcgtctcag
      421 cgcatgatct tcaggagccc ctgcggacca tggccagctt tgctcagctc ctggcgatcc
      481 ggtaccgggg caagctcgga gccgatgccg accagtacat cgaaatgatc atcagcgggg
      541 ccgcgcggat gcgggccttg ctcgaagatc tcctgactta ttcgcgcctg gcgcgccgcc
      601 agccgggtct cgagccggtc cacacagaac gcgtgttcca gagggcgctg gcgcgtctgg
      661 aaggccaaat caaacgggct ggagccattg tgacgtgcga tccattgccg gtcgtgcagg
      721 cagaccccgc ccgcctcgag ctcgtttttc agaatctgct gggcaacgcg gtgaaattcc
      781 gcgccggcgc tcggccgcga atccacgtct cggccgccag gaatggaaca gagtggattt
      841 tctcggtccg ggacaacgga atcggcatcg aggcgcagca tttcaccagg atctttctca
      901 tcttcgagcg cctgcacagc aagcaggagt atgccggcag cggcgtcggg ctggccatct
      961 gcaagaggat cgtagaagga catcgcgggc gcatctgggt ggaatccgag ccgggcaagg
     1021 ggtccacttt ctacttcact atccccatat tggaggaagc ctaacaggcc ggcgcggcat
     1081 cttaaaaacg ggcggtttct gctgcacccg agtagcagcg tcgggcgagc ccggattttg
     1141 cgaattgtta taattttgac gggcacggaa aatcatgcac actattgagt ccacgccgga
     1201 actggtcgag gaagtttacc ggaatatgga acgcaatctg gccgttgtgc ggaaacgcct
     1261 cggccggccg ctgagtttgg cggagaaaat tttgttttca catctggacg atcccgaggg
     1321 gcaggatctg acggctggcg tgagctatct gatgctgcgg ccggaccgcg tggccatgca
     1381 ggacgccacg gctcagatgg ccctgctgca attcggcctg gcgggccgcg agcacgtcgc
     1441 cgtgcctacc acggtgcatt gcgaccactt gattcaggcg cgcgctgggg cgaagaccga
     1501 cacgcaattc gccatcgtcg agaacaacga agtttacgaa ttcctgcgcc aggcttccgc
     1561 caaatacgga atcggcttct ggaagccggg atcgggcatc attcaccagg tggtgcttga
     1621 gaactacgcg tttcccggcg gcatgatgat cggcaccgac tcgcacacgc cgaatgccgg
     1681 cggattgggc atgttcgcgg cgggagtcgg cggagccgac gcagtggacg tgatggcggg
     1741 ctttccatgg gaagtgaaga atcccaagct gatcggcgtc cggcttaccg gcgagctttc
     1801 gggatggacc gcgccgaagg acgtgattct ctatctttgc ggcgtcctca ccgtcaaagg
     1861 cggcaccaac cgggtgatcg agtacttcgg ccccggcgcg cgcagcatca gttgcacggg
     1921 aaaggcgacg atcaccaaca tgggcgccga gcttggcgcg acgacttcga tctttcccta
     1981 cgatgagcac atggcggcct atctgcgcgc gacccggcga gcggctctgg ccgatctagc
     2041 cgggcggcgc cgcgaactgt tggaagccga tccggaagtc gagcagcatc ccgaaaaata
     2101 cttcgagcgc gtggtggaaa tcgacctttc ccggctcgag ccttacatgg tcggcccgca
     2161 tacgcccgat ctggcgcgcc cggtttccag gctggcggcg gacgtgaagg agcacggcta
     2221 tcctgatcgc atctccgtgg ccctgatcgg cagctgcacg aactcctcct acgaagacat
     2281 ctcgcgcgcg gcggatgtcg ccgagcaggc ccgggctcac ggagcccgga tgaaagcgcc
     2341 gtttctggtt acacccggct cggagcaggt gcgttccacc attgagcggg acggccagat
     2401 ggctgcgctc gaatcgatcg gcgccacggt gctggcaaac gcctgcggcc cttgcatcgg
     2461 ccagtggaag cggcaggaga tttcccaagg ggagccgaac acgatcgtaa cgtcgttcaa
     2521 ccgcaatttt cccaagcgca acgacggcaa tccggcgacg cacgcgttta tcggaagccc
     2581 cgagatcgtc atcgcctatg gtctggcggg aagactcagc ttcgatccgt tgcgggacga
     2641 gatcgaaacg ccggacggga agcggtttcg gttgcagcct ccccaaaaag ctcccgagtt
     2701 gcctcccaac ggtttcgtct cgaccgtgga aggctttgtg ccgccgcctg ccgacggcag
     2761 cagcgtcaag gttgagattg ctcccggcag cacacggctc caggccctcg agccgtttcc
     2821 ggcctgggac ggaaaagatt tccgcgaaat gcccatcctg gtcaaagcga agggcaaatg
     2881 caccacggat cacatttccc ctgccggccc gtggctccgg ttccggggac acctcgacaa
     2941 catcagcgac aatcttttct ccggagccat caacgcgttc acggggcaag cgggccaggg
     3001 aatcaacgtg ctcaccggtg aacggaatgt gcccttcgcg aaaatcgccc gcgattacaa
     3061 ggccaagggc ctgcgctggg tggctgtcgg cgatgagaac tacggagaag gatccagccg
     3121 cgaacacgcc gccatgtccc cgcggttttt aggcgccgcg gcgatcatcg tgcgcagctt
     3181 tgcgcgcatc catgaatcga atctcaagaa acagggcgta ttgcctctga ccttcgcgga
     3241 tccgtccgtg tacgacaggg ttcaggagaa tgaccggatc agcctgatcg gcctgaagga
     3301 tctggccccg ggccggccag tgaagggcat cctgcatcat gccgatggat cgagcgagtc
     3361 tttcgacctc aatcacaccc tcaaccggga gcagatggaa tggttcaaag ccggctcggc
     3421 tttgaacctg ctgcgcctgc aagcccagcc cgaaccggtg gccggctgag gggtccgaac
     3481 agaagccgct ttccggcgtc ttctccagga ttttccgcat tccagagccg cggctacgat
     3541 cgcgcgggga gcgtgaaata gaacgtcgcc cctttgcccg gttgggattc cacccagatg
     3601 cgcccgccgt ggctctggac aatcattttg cacagggcca gcccaactcc ggtgccggaa
     3661 tactgttgtt tgccgtggag gcgctggaag atttcgaaaa tcttttccgc atgagcggga
     3721 tcgatgccga ttccgttgtc gcgcacggcg aagagccatt cacttccttt tcgatcggcc
     3781 gagatgtgca cgcgcggcgg ctcctgtccc cgaaacttca gagcattgcc gatgagattt
     3841 gaaaataact ggccgagccg tgcgggatcc gcgaggatag ccggcagggg gccgtaggtg
     3901 acgacggcgg cgctttcgtc gatgacggcc cgcaggtttt caatcgcccg cgtgcaaacg
     3961 gcctggcagt ccgtcagcgc aacttctgcg ggccggcggg ccacccggga gtatgccagc
     4021 agatcttcga taagctggcg catgcgcgcg catccggcca cggcaagctg gatgaagcga
     4081 ttgccgtcct ggtccagctt gccctggtag cgctgcgcga ggagctgcgt gaagctcgcc
     4141 acggtgcgca gcggctcttg cagatcgtgc gccgaaacgt atgcaaagcg ctcgagttcg
     4201 gcgttgtagc gggccagctg ctcggccttc gccgccaact ccttttccga acgcttgcgc
     4261 tcggtgatgt cggtgaccat cttcaaactg ccggcgaatt gcccgttgtt gtcgaacatg
     4321 gggctggcgg aaacgatggc ccaaagctcg gcgccgtcct tgcggacaaa ccggacgtcg
     4381 tatcgctcgg cgccggtccg gctccgagaa aggagcttgg accggaaaga ctcccggtcc
     4441 tcctcgctca cgaagctcca gatccggcgg ttcatggttt cctgcaagga gtagcccagc
     4501 atgtcggcca ttgtctggtt gacgaaggtt gtcttgcctt cccggttggt gatccagatg
     4561 ccttccgccg ccgcttccac aatgccccga aaggtctctt cgctgcgatg cagcctttct
     4621 tccgccagtt ctttcgcttc gcgggcccgc ttcttttcga gcgcgctctc gatggccggc
     4681 cctagccgca ccgcccgctc cttgatcacg tagtcggctg cgccgttctt gatgcactcc
     4741 accgctgttt cttcgtcgag ggagccggtc acgataatga acggcgtgag aggcgcaagc
     4801 tccttggcca gggccatggc gctgaagccg tcaaagctgg gaagggagta gtcggagagg
     4861 atcaggtccg gcgcgaaggt ttgcagctcg cggataaagt cgtcgcgcgt ctcgacgcgt
     4921 ttggcggcga accggaactt ggctttgcgc agttcacgtt cgatcagggc ggcgtcggcg
     4981 ggcacgtctt ccagcatcag aatgcggagc gtctgatcca tggggaagca tcacacagcg
     5041 ggcttgttca gaagcatcca atagaagccc agttcggaaa ccgaccggat gaaatcgtcg
     5101 aattccaccg gtttaacgat atagctgttc actcccagcc ggtaacactc ggttacgtct
     5161 tggtcttcgt gagaagacgt gagtacgacc acggggatgg ttctggtgcg ctcgtcggct
     5221 ttgagcttgc tcaatacttc gaggccgctg accttgggaa gcttcaaatc gagaaaaatc
     5281 actctgggcc tgtgcaagtc cctgccgtcg ccgcggccga agatatgctc gagcgccgcc
     5341 gcgccgtcgt tgacgacgtg gacctcattg atgaggttat gtttgcgcag cgcacgcagc
     5401 gtgagggcta cgtcgtccag attatcctcg accagaagaa cttccagtgg ttcgccgtca
     5461 tccatggctt gttttctccg ccgggagcgt gaaatagaag gccgcgccct cgttgacttt
     5521 cccctccgcc catacccgcc cgccgtgccg ctgtacaacc cgctccacga tggctaggcc
     5581 tacaccggtt ccctcgaact cctcggacga gtggagccgc tgaaatacgc caaacagctt
     5641 gttcacgtat tgcatatcga aaccggcgcc gttgtccttg acatagtacg tattctgccg
     5701 cgatccgttc gaggagccgc cgatctcgat ggccgcgttt tccttgtgcc tggtgaattt
     5761 ggcggcgttg gacaacaggt tcgcccagac ctgatggagc atggcgcggt cgcctcgggc
     5821 gggcggcagg tcgccgaaac tgcactcgag tttgcgctcg ggcgccgccc gctggatctc
     5881 ttcgaaaaca gatctggcca gttcgctcat gttgacttcg accagcgcca tctctttacg
     5941 gcccagcttc gaaaatgcaa gcaggtcgtt gatcaactgc cccattcgcc cggcgccccg
     6001 ggcgatggat tcgaggtagc cccgcgcttc atcctcgagc tgcacgccgt aatcctccaa
     6061 caaggctttg gaatagccgc tgatcgcccg gagcggagcc ttcaaatcgt gcgataccga
     6121 ataggaaaag gcctccagtt gcttcatcgc atagtgaagc tgctcggtcc gctccgcgac
     6181 ccggcgctcc agctcggcgt tcagccggcg gacctcctct tccgcgcgct tgcgatccag
     6241 aatttcccgt tcgagattgc ggttggccgc ctcagactct ttcgcccggc ggcgcgccat
     6301 gagggcgaaa agaatcgccg cggcgagcag cagcaggagc agcaggatcc ctgccacgct
     6361 aaggaatccc aacgtcaggt gaaaagtcca cttcttgccg gagaggttca ggttgatgat
     6421 cccttcccga ggcttttgag cggggattcc cggccctccc atttgaatga tggccgccgg
     6481 gcagttcgac ataacgcccg ccggcggcgc agccccgagc agccagagcc agccggcaag
     6541 agtcgcggcc agccggagtc gatgaggaac gtccgataaa cgcatttcgc gcgcaatgtc
     6601 cgctcgtctg tttttatcgt cggacgcagg ggaaa
//
LOCUS       DASREK010000081         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2189635, whole genome shotgun sequence.
ACCESSION   DASREK010000081 DASREK010000000
VERSION     DASREK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189635"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1490)
                     /locus_tag="VFA54_04340"
     CDS             complement(312..1490)
                     /locus_tag="VFA54_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50065.1"
                     /translation="MERQKPIARLAGAALGTAAVLIWQCLTVHYNYQGNWTALFYTGS
                     YNAGPPSLAQENIYTFPDSIGYDGQFYHYIAHDPLLRTGIRAHVDNPRLRWRRILVPG
                     LAFLLAAGRPESVDAAFFAVTAGFFFLGVYWSSRFCERCGINPGWGLCFGLIPGVLTS
                     IDRMTIDIALLALCAGFALYGAAEPSRKVLLILALAPLARETGVSLILGYCLSLAIRK
                     QWRPALYSLLAALPWLGWVIFVHQRTDPDLTVWGSVYPLAGIIGRTLQPVQFALTGRW
                     LVAAATLDYIGLLGIWAALFLAVRLAAMAAKAPFGPLEAAIVCAALPVLFAGKSDIWS
                     GGYEFGRTMSPLLALLALQGVRSRWWWNLLPLCMTAPRILFQLEPQWKGVIRGMLRAL
                     "
     gene            complement(1595..2644)
                     /locus_tag="VFA54_04345"
     CDS             complement(1595..2644)
                     /locus_tag="VFA54_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS50066.1"
                     /translation="MKIGIIGAGGVARGVHIPALRRIDGVEIAGIAREHKSEPVDGIP
                     VTKHYQDLLARADIGAIINTTPNHLHRQITLDALAAGKHVLCEKPLGLNHGETLEMLA
                     AAEKSGLVHMTAFTFRYAPGVQYLKHLIETGALGQIRSVRAAYLMALSEHLTGWRSQR
                     PLAGSGALADIGSHAIHIAQFLAGQIAAVSAAGRSFYSDVDDWIGFLAQFACGAMATF
                     EISRAAPGRGDEITENMFIEIYGTRGGAAFSMQDPWALMLCIGERARDAAANLERTPA
                     PQEFLKLPGSPRDIHADDPRWGYRYDQDFQFVDSIRQREVREPSFRAGAECQAVLDAV
                     LKSCEDGRWTPVAAP"
BASE COUNT          596 a          869 c          890 g          492 t
ORIGIN      
        1 cgagcggcag aatatcgcgg cggataaaaa ctacatgtcg agcgttcccg gcgtattcgc
       61 tgccggagac gcacgccgcg gccagtcatt ggtcgtatgg gcgctctcgg aaggccgcaa
      121 agccgcccgc gccatcgatc aatatctgat gggggagagc aagctgccgg cctgaagtct
      181 ggcattcgcg tgcaagtctg acaacagcgc ttcgggttcc cgcgtaagcg ttggaacacg
      241 catgggaagc cctctttgcc gcaaggtgag agggatgtat gatcgcctct gacgccctgc
      301 gaatcggcgg ttcacaaggc gcgcagcata ccccggatta cgcccttcca ttgcggctcg
      361 agctgaaaca gaatgcgagg ggctgtcata cacaagggca gaaggttcca ccaccatctg
      421 gaccggacgc cctgcaaagc cagcaaagcg agtagagggg acatggtcct gccgaattca
      481 tagccgcctg accagatgtc gcttttgccc gcaaacagaa ccggcagagc tgcgcaaacg
      541 atggccgctt cgagcgggcc gaagggcgct ttggccgcca tcgccgccag ccggacagcc
      601 agaaacagcg cggcccaaat tcccaggaga ccgatgtagt ccagagtcgc cgctgccaca
      661 agccatcggc cggtgagggc gaactgcacc ggctgcaatg tccggccgat tatgccggcc
      721 aacggataca ccgagcccca cacggtgaga tcgggatctg tcctttgatg cacgaaaatg
      781 acccagccga gccagggtag agcggccagc aggctgtaca gcgccggccg ccactgtttt
      841 cgaatcgcaa gagagaggca atatccgaga atcagagaca cgccggtctc tcgtgccagg
      901 ggcgccagcg ccaatatgag cagaactttg cgggatggtt cggctgctcc atacagggcg
      961 aatcctgcac acagggccag cagggcgatg tcgatggtca tccggtcgat cgaggtcagc
     1021 accccgggaa tcagcccgaa acaaaggccc cagccgggat tgattccgca gcgctcgcaa
     1081 aaacggctgg accagtacac gccgagaaaa aagaagccgg cggtgaccgc gaagaaggcg
     1141 gcatccacgc tctctggccg cccggcggcg agaagaaatg ccaagcccgg aaccagaatg
     1201 cggcgccatc gcagcctcgg gttgtcgacg tgtgcgcgga tgccggttcg caagagcgga
     1261 tcatgcgcaa tatagtgata aaactgcccg tcgtagccta tgctgtccgg gaaagtatag
     1321 atattctctt gagcgaggct tggcgggccg gcgttgtaag agcccgtgta gaacagcgca
     1381 gtccaattgc cctggtagtt gtaatgaacg gtcaaacatt gccagatcag gacggcggcc
     1441 gttcccagcg cggcgcccgc taggcgcgca atcggcttct gtcgttccac tcagcgcaat
     1501 ctagcacacc ggcgggggcc ggtgatctgc ggattttcac cattggcgca ggcgccgcga
     1561 gacgcgagaa aagcgccgtt ccggcttctg cgaatcaagg cgcggcgaca ggcgtccaac
     1621 gcccgtcttc acaggatttc aagacggcat ccaacaccgc ctgacactcg gcgccggccc
     1681 ggaaggaagg ctcccgaact tctctctggc ggatgctatc gacaaactga aaatcctggt
     1741 cataacgata gccccaccgc gggtcatcgg cgtgaatatc gcggggcgag cccggcagct
     1801 tcaggaattc ctgcggcgcc ggcgtgcgtt ccagattggc cgccgcgtcg cgcgcccgct
     1861 ccccgatgca gagcatcagc gcccaaggat cctgcatgga aaaggccgcg ccgcccctgg
     1921 tcccgtaaat ttcgataaac atgttttcgg tgatctcgtc gccgcgtcca ggcgccgcgc
     1981 gggaaatctc gaatgtggcc attgcgccgc acgcaaattg cgccagaaag ccgatccagt
     2041 cgtcgacgtc ggagtaaaag gaccgtccgg cggcggaaac cgctgcaatt tggccggcga
     2101 gaaactgggc gatgtgtata gcgtgagagc cgatgtccgc gagcgcgcca gaccccgcca
     2161 gaggccgctg cgatcgccag ccggtcagat gttccgagag cgccatcagg taggcggccc
     2221 gcacgctgcg gatctgtccc agtgcgcccg tttcaatcag atgcttcagg tattgcacgc
     2281 ccggcgcgta gcggaacgtg aacgcggtca tgtggaccag tccggacttc tcggcggctg
     2341 ccagcatttc cagcgtctcc ccgtgattga gccccagcgg cttctcgcag agaacgtgct
     2401 tgccggcggc cagggcgtcc agagtaatct gccgatgcag gtggttgggc gttgtgttga
     2461 tgatcgcgcc gatatccgcg cgggcaagca gatcctggta atgctttgtg actggaatgc
     2521 cgtcaaccgg ttctgattta tgctcgcggg cgatacccgc aatttcgact ccgtcgatcc
     2581 ggcgcagggc ggggatatgg acgccgcggg ccactccgcc cgctccaata atgccgattt
     2641 tcacaggtgt gaggtccatt atagagtgaa aaatcaatac tacactatgc gcgagcccgg
     2701 cgcgccgaag gccgctcgat tgcgcggcaa gctcccgaag cttccggcga aactcacccg
     2761 ccctaatgtt ctgcgatttt tctccggctg caattctctc tttgctatgt tagggcttga
     2821 ccggagggga tgtggcgagg aagccgg
//
LOCUS       DASREK010000082        13427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2189866, whole genome shotgun sequence.
ACCESSION   DASREK010000082 DASREK010000000
VERSION     DASREK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189866"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VFA54_04350"
     CDS             complement(<1..1413)
                     /locus_tag="VFA54_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50067.1"
                     /translation="MAYTPATAVEIDKKLRDDFRRRLKDFGISAETTDPVLAVLFRTF
                     AGQLEALYSETERIRLALLDELISGLGLERRMARPAQTIVRFLLDAGSELVEAGTELI
                     GEAQSGERLTFTTDKDIFVSTAAIALAATYQEGALRLMPGVETSEQIQAARPALEPVR
                     VNLGPNPAVFLAIENLPPHHLSRHGFFFELSPDAESIRRAMLSETWCLAGPEGELGAR
                     GILRPRRLNAGVRELCWLIDGALPEPSRAAESETPPLPDGFYAGRIFIFPETPPDRQF
                     LCRLPKATETAFNKIFGRAAAQIFARPRAWLRISFPRRVADLHTGISSISLHAVSASN
                     VECFNQTVRFEEHGASIPISREGGTSKFLVAPLSVFGEAKSAYLPEFQPSPDPDAGRY
                     HIHGGRIELRPARHVDGHFDSYANLRLWVTSGSLGNKVGPGKLQTFLKKSGSPSLRVS
                     NPTSAAGGTDGETAEQARERF"
     gene            complement(1417..1767)
                     /locus_tag="VFA54_04355"
     CDS             complement(1417..1767)
                     /locus_tag="VFA54_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50068.1"
                     /translation="MSFIAFPLRLNKSFLQRCDEVDAILALIRVMAVTPNGSWAGSSH
                     FGLRDLFEQARMHPELPAQAVQEVNRALEDLGITHYRVQAIVKDPQAKQDEDSYTVTL
                     ISSDADSQTVSRKV"
     gene            complement(1834..2283)
                     /locus_tag="VFA54_04360"
     CDS             complement(1834..2283)
                     /locus_tag="VFA54_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50069.1"
                     /translation="MAASPYRSTVTIDGTKFDAVSVEVVFSTQRDRAGMPEMGSLQSK
                     IRVWADAHDDKNLPHSAIQKFFDLANVVTRDKIKDMKIEFWKDDAHQDALCSFKFKGW
                     ISAFETSNPANPPDPGDGWRPYLNHALVLDLEPAMNDQHFQDISISN"
     gene            complement(2311..2784)
                     /locus_tag="VFA54_04365"
     CDS             complement(2311..2784)
                     /locus_tag="VFA54_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50070.1"
                     /translation="MAATPYRCTVTIDGTKFDAICSKVRFTTDKDRAGMPMMGSLKTT
                     IAVFADMHDDKNLPFSAIKKFFDLANVVTRDKIKAMKIENWKDDAHQDALWSYSFQGW
                     IRRFEVSNPHPAGYGDLDGAMDWGDAVPHDVNHLLVLELEPVLNQANFAEIKLSN"
     gene            complement(2824..3177)
                     /locus_tag="VFA54_04370"
     CDS             complement(2824..3177)
                     /locus_tag="VFA54_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50071.1"
                     /translation="MDRPFKVEAGSAAKAAEDEARAQRSGNAGAAKQALRLTPRASSV
                     PTPEVALREQFRASHEIHISAADGRRFDLPLPTQDEAAARQAVPEAAAGERTEPASAP
                     PSLKQKLFKFLGWKH"
     gene            complement(3197..4540)
                     /locus_tag="VFA54_04375"
     CDS             complement(3197..4540)
                     /locus_tag="VFA54_04375"
                     /inference="COORDINATES: protein motif:HMM:NF036663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50072.1"
                     /translation="MPEEVATKTRTTAEILGESAARDLLQDIFADLHPGLIDETRDIA
                     SIFTRQSASEFLSQLTDFEKLLSAEDSYEQTVKKMQEQIDKAEKMRDQLLAQVFQKIR
                     PIERSYRELMLFFENSKVPDGKVRKPVELYIFNADTKAMKDVFSMNIAAFENFVRKRN
                     DNFNFRDDICNVVVPGHVPQPVREKFEDIANAWGMLLIGDIDDEKSFKNIERNFLPGG
                     KYEFMKRPEDRAAADVVTMGYLQLRPAHWFEKEVDGGDDLYGPPSLVFAGAVARADDA
                     VGIAQGPVGSRFGQIAGAEKARVEPLIGEMEHLSMERQLIPIIRDADNHLCFFGCRTQ
                     AEDPYGVLKFFTSYRILRYLERCCRHYLLQVAGQVLTREFMDEQIEAPLKRLLDEQVE
                     QGTILGYDLFVDKDSNKRMQGICDITLNVMPTGPAETFVLKIDVPEFKKQTAEKN"
     gene            complement(4533..5048)
                     /locus_tag="VFA54_04380"
     CDS             complement(4533..5048)
                     /locus_tag="VFA54_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50073.1"
                     /translation="MPLVKVIRKGITPAVRKVPTSTCAAIMPMTLERVGQEVASENEE
                     PTKIESAQDAFEKFQPRLDFKGYAGEDETEFKAEFRFRSIKDFDPENLQKRQEIRNAD
                     GEVEYLRNDIADLKSNIDLLYRLKDRWKLPTVRRAWSNPNQRKEIVSALGKLRSELEK
                     IAGKEGGKANA"
     gene            complement(5127..6608)
                     /locus_tag="VFA54_04385"
     CDS             complement(5127..6608)
                     /locus_tag="VFA54_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HZS50074.1"
                     /translation="MTFQGARRAGPGIFFALLSAAVVWLAPEAWAQGQTAHLEFSDKL
                     RLVNCEPTSSSPCFRIRANIVDAQGAPVSPALPPPAQLAKNLTVRADDLEVTPFYAVA
                     GSSGATVARSRIALILIDISGSMNEPMGTGQSRFAAATSAALQFLDGFENGTDRVAIV
                     PFESHQVIQTIRAAKFASTVEEARQQVRSLPAPQPKNNTALFSAVDASLDVLAAQMAA
                     AQTSPETMLVVMTDGKNDVRQGDDLGLLSGPSGLATVANKVKASGIQVVSVGIGDNQS
                     IDSDAMRQISTQFYLVSDAESLKRVFSLARKLLVNRIQATFTSPWADRASLAGKTLHV
                     RLSLKLANGEQISSNEIVWSTPQMGVPLFEGKCEAPEMKALLAQATPPSTGGWWALLR
                     PVLVFVSIGVTILVMWFWVPRLVWPEQYLGELPATASGGRWSGQTQIRRTTTGPRRNA
                     PPGFDATSAGRAGAQRGPGDATIVRPRTDFGTRTRLEGRRQRE"
     gene            complement(6605..7561)
                     /locus_tag="VFA54_04390"
     CDS             complement(6605..7561)
                     /locus_tag="VFA54_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZS50075.1"
                     /translation="MSLYQTLYYMSLVGSMAGLFSWGLVALISAALPSQETIWVADLV
                     STTILGAFIGGLTVAFTDRWAGSRVMPRWVVSGTVIGMAAGLVAGGIQIPITNNLAEQ
                     APVITRVIAWMLAGSLIGLGVGLRWVAVNSTRAAHAFAGGLLGGAMGGLLFAALGSRI
                     PDLSQALGFVLTGVGICFGITLAPILLRDGVIQFVSSGDPRAQSKFGRSRKEWELQQG
                     DTYVIGSQSQDLSKTRYRPDVEIFIPDAAIAPRHATLFGRERRFYVARHPDAGGPAGL
                     ARYVLRVRGKTVTTTQELHDSDDILIGRTALKFITRKKSDQG"
     gene            7941..9062
                     /locus_tag="VFA54_04395"
     CDS             7941..9062
                     /locus_tag="VFA54_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZS50076.1"
                     /translation="MENTAFERRRLRQLQAIVVGAGALGNEVVKALGLLGVGKMMIVD
                     PDIVEPSNLTRSILFRTSGATGSNKARALAQAAAQIFPDTEIEAVEDEIAGLGFRPIA
                     AADILFSCVDSESARLEIAYIATKLDRPVCDAGLGGPNYSHGRVTFFPGRPAACYCCG
                     LSGRKRRELLTTWQSAVRSCWTSAAAVEQRPYPSTPTMAAVIGAMQVEIGLRRLLDSA
                     SEAWSIELTLALPVKAETFTLRRSEACPFHAPEACMISERDAEAVRGGNSRGLTIADL
                     LASAPAVAGTEPVLLLDWPICVQARCGACRERWPPMQRVTVIRRRGACPHCGSNQVTE
                     EETISRVSRSAPWAQLTPAELGIPEQHLHTIRFERDQPQ"
     gene            9059..9805
                     /locus_tag="VFA54_04400"
     CDS             9059..9805
                     /locus_tag="VFA54_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50077.1"
                     /translation="MSGRKATIVAASCFLLAVLQLLAALVNPAASVGAAVVVGVLTFG
                     ILRLFDVELERAWSPLLIAAGSSAAAIGVRYFGSPATADRTLGLSPLVAFVAAAMVLL
                     LRRSKAQRCGLCNRRIGRSLAFECPRCGLTVCDQTCWIFEHCRCRLCEQNRVPVFSPD
                     GRWWDRRFGPRMKQGRCQLCLAEAADADLRACANCGRPQCRPCWDFANGQCSRCQWTV
                     DDLPEGLKMYMLPGEPGAGRDAPDRRAPIR"
     gene            9811..10071
                     /locus_tag="VFA54_04405"
     CDS             9811..10071
                     /locus_tag="VFA54_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50078.1"
                     /translation="MAKYQMYFDTIKGETRYNVDIDEDETLDAVLDEILYDLRERGDV
                     LQGDGEPQVVWNGTALDFTVTLPEQGVRPNDVLRVSTVATNG"
     gene            10064..10696
                     /locus_tag="VFA54_04410"
     CDS             10064..10696
                     /locus_tag="VFA54_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50079.1"
                     /translation="MGNTLSRQTSPRPLSAPGTERPAAPRDRRLEQEWKLLGQLAQTN
                     PGVLEVLRRRSLADSDIFEAMIHQTCGILSLARGKPVLARSHRVAFRFPRFFPSVPIE
                     AVLAKPVFHPNVDPRNGFVCLWTRTSAADTTMEAARRLQQMIAWKLVNFEATHVMQPE
                     AVEWARRMSFPAGLALDFTPLVEVESFRREKSFTARPDAPFRRRLEPLPG"
     gene            complement(10779..12086)
                     /gene="senA"
                     /locus_tag="VFA54_04415"
     CDS             complement(10779..12086)
                     /gene="senA"
                     /locus_tag="VFA54_04415"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HZS50080.1"
                     /translation="MRATELETMLRDARRRTLELVCDLTEAQMRVPLLAIINPPVWEI
                     GHVAWFQEKWVLRHLLGKDPIRPDGDAIWDSAVIAHDDRWSTLPRSATLRYMQEIFDR
                     VIDSLPQGDVADSEAYFYWYWLAVMHEDMHGEALMYTRQTLGYPEPELALEGTAGLGQ
                     ETGGGWDGPEAEGDVFMPGGLFQLGAKPGKTFVFDNEKWAHAVKVQSFAISRTPVTNR
                     QFAEFVNDGGYRRRGLWSEEGWSWREKAQAEHPVYWIPQTGGRWMRRHFDQIVPLGED
                     LPVIHVNWYEAEAYCAWAKRRLPAEAEWEMAASCESPSSGKKREFPWGDERPTAERAH
                     LDARTLGCVPASDLAAGDSAAGVRQLIGNVWEWTASDFQPYPGFVVDPYKEYSQPWFG
                     AGRKVLRGGCWATRSRLIRNTWRNFYTKDRRDVFAGFRTCAAD"
     gene            12124..13077
                     /gene="senB"
                     /locus_tag="VFA54_04420"
     CDS             12124..13077
                     /gene="senB"
                     /locus_tag="VFA54_04420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HZS50081.1"
                     /translation="MSCPAPPGSLYGNRVTALRWSRILRELGHKVSIVPAYENQPCQL
                     LVALHARRSAASVFAFQEQHPGKPVILALTGTDVYRDIHRLQRARRALELASRLIVLQ
                     PLAVNELPANLRPKARVIYQSAERTAGGRRRTDFFDVCVVGHLRGVKDPFRAAYASRL
                     LPKESRIRILQAGAPMEERMAQMARSEQVRNPRYRWLGGLPRGRARRLIRSSRLVVLS
                     SRMEGGANVISEAVADGTPVLASRISGSVGLLGERYPGYFPVGDTAALAALLLRAESD
                     CAFYANLTQWCLRLRPLFRPAAEKAAWAKLLQEVCPASRPD"
BASE COUNT         2447 a         4224 c         4057 g         2699 t
ORIGIN      
        1 gaagcgctct ctggcctgtt ccgcagtctc tccatccgtg ccgccggccg ccgacgtagg
       61 attgctgacc cgcagagagg gcgacccgct cttcttcaga aacgtttgta atttgcctgg
      121 accaaccttg ttgcccagcg acccgctggt aacccagagg cgcagattgg cgtagctgtc
      181 gaaatggccg tccacgtggc gggccggccg tagctcgatg cgcccgccgt gaatgtgata
      241 ccggccggcg tcggggtccg gcgacggctg gaattccggc aagtaggccg acttggcctc
      301 tccaaaaacc gagagcggcg caaccagaaa tttcgatgtg ccgccttcac ggctgatggg
      361 aatggaagct ccgtgttcct caaagcgcac cgtctggttg aagcactcga cattcgacgc
      421 gctcactgcg tgcagagaga tggaactgat gccggtatgc agatcggcga cgcggcgcgg
      481 aaagctgatg cgcagccatg cccgcggccg ggcgaaaatc tgggccgccg cccgtccaaa
      541 aatcttgttg aacgccgtct cagtggcttt cggcaggcgg cacagaaact ggcgatccgg
      601 cggcgtctcg gggaatatga aaatgcgccc ggcgtaaaat ccgtccggca ggggcggcgt
      661 ctcgctttct gcggcgcggg acggctccgg aagcgcacca tcgatgagcc agcaaagctc
      721 ccgcaccccc gcattcagac ggcgcggccg caggatgccg cgagctccca gttccccttc
      781 gggtcccgcc agacaccagg tttccgacaa catggcccgg cgaatgctct cggcgtcagg
      841 cgatagctcg aaaaagaaac cgtgccggct caagtggtgg ggcggcaggt tttcgatggc
      901 tagaaaaaca gccgggttgg gtcccagatt tacgcgcacc ggctccagcg ccggccgcgc
      961 cgcctgaatc tgctccgacg tttccacgcc tggcatcagg cgcagcgcac cctcctgata
     1021 tgtcgccgcc agggcgatgg cggcggtgga gacgaaaatg tccttatcgg tggtaaaggt
     1081 caggcgttcg ccggattgcg cctctccaat cagctcggtg ccggcctcga ccagttccga
     1141 tcccgcgtcc agcagaaatc gcacaatggt ttgcgccggg cgggccatgc gcctttccag
     1201 tcccagaccg gagatcagtt cgtccagcag cgccagccgg atgcgttccg tttccgagta
     1261 aagggcttcg agttgtccgg cgaatgtacg gaacagaacc gcgagaaccg ggtcggtggt
     1321 ttcggcggaa atgccgaagt ccttcagccg ccgccgaaaa tcgtcccgca gttttttgtc
     1381 gatctcaact gcggtcgccg gagtatacgc catgttttag accttgcgcg aaaccgtctg
     1441 gctgtccgcg tcgctggaaa tgagcgtgac cgtgtaggaa tcttcgtcct gtttggcttg
     1501 gggatccttg acgatcgcct gcacacggta gtgtgtgatt cccaggtcct ccagcgcccg
     1561 gttgacttcc tgaaccgctt gagccggcag ctccggatgc atccgcgcct gttcaaacag
     1621 atcgcgaagc ccgaagtgag acgaccccgc ccagctacca ttgggcgtga cagccattac
     1681 acgaatcaaa gccaaaatcg catccacttc gtcgcagcgt tgcaggaacg atttattcag
     1741 gcggagaggg aatgcgatga acgacataat tttccgggtc ttcactgccc ggttttgtta
     1801 aagcaaagcc ggcggccccg aatcgagaaa ccactaattg ctgatcgaaa tatcctggaa
     1861 atgctgatca ttcatcgccg gctcgaggtc cagcacgagc gcgtgattca gatacgggcg
     1921 ccagccatcg cccgggtcgg gcggattcgc cgggttgctc gtctcgaagg cgctgatcca
     1981 gcctttgaac ttgaaactac acagagcgtc ttgatgcgca tcgtctttcc agaactcgat
     2041 cttcatatcc ttgattttgt cgcgggtgac gacgttcgcc aggtcgaaga acttttgaat
     2101 agcggagtgc ggcagattct tgtcgtcatg ggcgtcggcc cacacccgga tcttgctttg
     2161 caagcttccc atctcgggca tgccggctct gtctctctgg gttgagaaaa ccacctccac
     2221 ggagacagcg tcgaatttcg ttccatcaat ggtaaccgtg ctgcgatagg gtgatgctgc
     2281 cataagtcct cctgctcagt gagcgctgcg ttaattgctc agcttgattt cggcgaaatt
     2341 agcctggttc aataccggtt ccagctccaa caccaacagg tgattcacgt catgcggtac
     2401 tgcgtcgccc cagtccatgg cgccgtcaag atcgccatag cccgccggat gcggattgct
     2461 cacctcaaac ctccggatcc agccctggaa gctgtagctc cacaaagcgt cttgatgggc
     2521 gtcgtccttc caattctcga tcttcatggc cttgattttg tcccgcgtga ccacgttggc
     2581 cagatcgaaa aatttcttga tcgccgagaa aggcagattc ttgtcatcat gcatatccgc
     2641 aaaaaccgcg atggtcgtct tcaagctgcc catcatcggc atgccggccc ggtctttatc
     2701 cgtggtgaag cggacctttg agcaaatcgc atcgaacttg gtgccatcga tggttaccgt
     2761 gcaccgatac ggcgttgctg ccataatcca acctcctgtg cttcagcaat cgaaattaca
     2821 tccttaatgc ttccaaccca agaatttgaa aagcttctgc ttcagggaag gcggcgcgga
     2881 agccggctcg gtccgctctc cggcggcggc ttcgggtacg gcctgcctgg cggccgcctc
     2941 gtcctgcgtc ggcagcggca ggtcgaatcg ccgcccgtcc gctgcgctga tgtgaatctc
     3001 gtgcgaggcc cgaaattgct ccctcagcgc gacttccggc gttgggaccg aggaagcgcg
     3061 cggcgtcaaa cggagagcct gcttagcggc ccccgcattc ccgcttctct gcgcgcgggc
     3121 ttcgtcttcg gcagccttcg ccgccgatcc cgcctcgacc ttaaagggac gatccatagc
     3181 agacctccgc gagttttcaa ttcttctcag cggtttgctt cttgaactcc ggcacgtcga
     3241 tcttcagaac aaaggtctcg gccggtccgg tcggcatcac attgagcgtg atatcgcaga
     3301 ttccctgcat ccgcttgttc gagtccttgt ccacgaacag gtcgtagccg aggatcgttc
     3361 cctgctccac ttgctcgtcc agcagccgct tgaggggcgc ttcgatctgc tcgtccatga
     3421 actcgcgggt gagcacttgg ccggccactt ggagcaaata gtggcggcag caacgctcga
     3481 ggtaacggag aatgcggtac gacgtgaaga acttcaacac gccgtatgga tcttccgcct
     3541 gcgtgcggca gccgaagaaa cacaaatggt tgtcggcgtc gcgaatgatg ggaatgagct
     3601 ggcgctccat gctcaaatgt tccatctccc cgatcagcgg ctcgacgcgc gctttctcgg
     3661 ccccggcaat ctgcccaaag cgcgaaccga ccggcccttg cgcgatgccc acggcgtcat
     3721 cggcccgggc cactgcgccg gcgaaaacca gggaaggagg cccgtacaaa tcgtcgcctc
     3781 cgtccacctc tttttcgaac caatgcgcgg gccgcaattg cagatagccc atggtcacta
     3841 catcggccgc ggcccggtcc tccgggcgct tcatgaactc gtactttccg cccggcagga
     3901 agttgcgctc gatgttcttg aacgactttt cgtcgtcgat atcgccgatc agcagcatcc
     3961 cccaggcgtt ggcgatatcc tcgaacttct cgcggaccgg ctgcgggaca tggccgggga
     4021 caaccacgtt gcatatgtcg tcgcggaaat tgaagttatc gttcctcttg cggacgaagt
     4081 tttcaaacgc cgcgatgttc atgctaaaaa cgtctttcat cgcctttgta tcggcgttga
     4141 agatataaag ctctaccggc tttctcacct tgccatcggg caccttgctg ttctcaaaaa
     4201 acagcatcag ctcgcggtag cttctctcga tcggacggat cttctggaag acctgcgcca
     4261 ggagctggtc gcgcatcttc tctgccttgt caatctgctc ctgcatcttc ttgaccgtct
     4321 gctcgtagct atcctctgca ctgaggagtt tttcgaaatc ggttagttgc gataaaaact
     4381 cgctggccga ctggcgggtg aaaatgctcg cgatatcgcg ggtttcatcg atcagccccg
     4441 ggtgcaggtc ggcgaagata tcctgcaaca aatcccgggc ggcgctctcg cccagaattt
     4501 cggcagtggt cctggttttg gtggcgacct cttcaggcat tagctttgcc tccttccttg
     4561 ccggcgatct tctcgagctc gctgcgaagc ttcccgagcg ccgaaacaat ttcctttcgc
     4621 tggttggggt tgctccaggc ccggcgcacg gtcggcagct tccagcggtc tttcagccgg
     4681 tacaggagat cgatgttgct cttcaggtcg gcgatgtcgt ttcgcaggta ctcgacctcg
     4741 ccgtcggcgt tccggatctc ctggcgcttt tgcaggttct ccgggtcaaa atccttgatg
     4801 ctgcgaaaac gaaactcggc tttgaactcg gtttcatctt ctccggcgta gcctttgaaa
     4861 tcgagccgcg gctggaattt ttcgaaagca tcctgcgcgg attcaatttt ggtcggctcc
     4921 tcgttttcgg aagccacctc ctggccgact cgttccagcg tcataggcat gatagccgcg
     4981 caagtggacg ttggcacttt gcgcacagcc ggcgtgatac ctttgcggat cactttgacc
     5041 agcggcatat ttgattacct ccaatactca gtactgcttt gatccatcct gaccacccgt
     5101 ttcagattct gcgagcctgc tgcttgctac tcccgttgcc tccttccttc caggcgggtt
     5161 cgggtcccga aatcggtccg cggccggaca atcgtcgcgt ccccgggccc tctctgagcg
     5221 cccgcacggc cggcggacgt agcgtcaaat cccgggggag cgttccggcg cggccccgtg
     5281 gtcgtgcggc gtatctgcgt ttgccccgac cagcgcccgc cggatgccgt tgcgggcaac
     5341 tctcccaggt actgctccgg ccaaaccagc cggggcaccc aaaaccacat caccagaatc
     5401 gtgaccccga tcgaaacgaa taccagtacg gggcgcagca gcgcccacca cccgcccgtg
     5461 cttggcggcg tcgcctgcgc gagaagcgct ttcatctcag gcgcttcgca tttcccttcg
     5521 aacagcggca ctcccatctg cggcgtggac cagacgatct cattgctgct gatctgctcg
     5581 ccgttcgcca gcttcaacga cagccggaca tgcagcgtct taccggcaag agaggcccgg
     5641 tccgcccagg gggatgtgaa cgtggcctgg atgcggttca ccagcagctt gcgcgcgagc
     5701 gaaaaaaccc gcttcaggct ctcggcgtcc gaaaccaggt aaaactgcgt gctgatctgc
     5761 cgcatggcgt cggaatcgat ggactggttg tccccaatcc ccaccgacac cacttgaatg
     5821 cccgacgcct taaccttgtt tgcgaccgtg gcgaggccgg aaggtccgct gagtaaaccg
     5881 aggtcatcgc cttgccggac gtcgtttttc ccgtcggtca tcacgaccag catggtttcc
     5941 ggcgaggttt gtgcggcggc catttgagcc gccaggacgt ccagcgaggc gtctacggcg
     6001 gaaaataacg ctgtgttgtt tttcggctgc ggcgccggca gcgagcgcac ctgctgccgg
     6061 gcctcctcga ccgtactcgc gaacttagcg gcacggatgg tctggatgac ctggtgactc
     6121 tcaaacggaa cgatcgctac gcggtccgtg ccgttttcaa agccgtccag aaattgcaaa
     6181 gccgccgaag tggcggccgc gaatctggat tgccccgttc ccatcggctc gttcatgctg
     6241 ccgctgatat caatgagaat cagggcaatt cggctgcgcg cgaccgtggc cccagagctt
     6301 ccggctaccg catagaacgg ggtgacctcg aggtcatccg cgcgcaccgt taaattcttg
     6361 gcgagttgag caggaggcgg cagtgcgggg gagacgggag cgccttgggc gtctacgata
     6421 ttcgcgcgga ttcgaaaaca agggctgctt gaggtgggct cgcagttcac cagccgcagc
     6481 ttgtccgaga attcgagatg agctgtttgc ccctgcgccc aggcttccgg agccaaccat
     6541 acaaccgcgg cgctcaacag ggcgaagaaa atccctggcc cggcccgcct ggcgccttgg
     6601 aacgtcatcc ctggtcggac ttctttcggg taatgaattt caatgccgtt cggccaatca
     6661 gaatatcgtc ggaatcgtgc agttcctggg tggtggtgac cgttttgccc cgaacacgca
     6721 gcacatagcg agccagccct gccggaccgc cggcgtcagg atggcgggcc acgtaaaagc
     6781 gtctctcccg cccgaacagc gtagcgtggc gcggggcgat tgcggcatca gggatgaaga
     6841 tctccacatc cggacgatat cgggttttgc tcaggtcctg gctctggctc ccgatcacgt
     6901 aagtgtcccc ttgctgcagt tcccattcct tccgcgaacg cccgaacttg ctctgggctc
     6961 gcggatcgcc gctggagacg aattgtatca caccatcccg aagcagaata ggagccagag
     7021 tgattccgaa gcatatgccg acgccggtca gaacaaaacc aagggcttgc gagaggtcgg
     7081 gaatccggct gcccagcgcg gcaaagagca atccccccat cgcgccgccc aacaagccgc
     7141 cggcaaaggc atgggccgcc cgggtactat tgacggctac ccagcgcagc ccgactccca
     7201 gcccgatgag cgatccggcg agcatccagg cgatcactct ggtgattacc ggcgcttgct
     7261 ccgccaagtt gttggtaata gggatttgaa taccgcccgc gaccaacccg gccgccatcc
     7321 cgatcaccgt tccggagacc acccagcgcg gcattacccg gctgccggcc cagcggtcgg
     7381 tgaaagcgac cgtcaagccg ccgatgaagg cccccaagat ggtcgtcgag accaggtccg
     7441 cgacccagat ggtctcctgg gagggcaggg cggctgagat taaagcgacc agtccccagg
     7501 aaaaaaggcc cgccatacta cccaccaggg acatgtagta gagcgtctga tacagactca
     7561 tcggccgggc ttccccactg cgggatcgac agtataccaa aagcgcccac gagtaaagca
     7621 caaagcatgt agtcacaggt tttgccgagc gaagcgatcc ccagggccga ggcttgcggc
     7681 cggaagcaac aggcctttcc ggcctggaac aatgtgtttc gcaaactgcc ggcccggggg
     7741 gtctgtcttt ttttttccac attcaggatt gcttcgaatc gcaccagccc ggcgcgcgcc
     7801 gcttttcgag caacgttcca gccagccgcc ttccacaacg ccggcgcact tgaacccgct
     7861 tggtacaatg attcccggtg agcctgccaa ccgttccgca tcccgagccg gcgcaatctt
     7921 ggcgtgcaga ctattcgagg ttggagaaca ctgcctttga aagacgccgc ctgcggcagt
     7981 tgcaggcgat cgtcgtggga gccggagctc tgggcaatga ggtggtgaaa gccctcggat
     8041 tgctcggcgt cggcaagatg atgattgtcg atcccgatat cgttgagccg agcaacctga
     8101 cacgttccat cctgtttcga acttcggggg caaccggatc gaataaagcg cgcgcgctgg
     8161 cccaagccgc cgcgcaaatc ttccccgata cggaaatcga ggctgtggaa gatgagatcg
     8221 ccggcctcgg gtttcgcccg atcgccgcag ccgatattct gtttagctgc gttgacagcg
     8281 agtcagcccg gttggaaatt gcgtacatcg cgaccaagct ggaccggcct gtgtgcgacg
     8341 ccggattggg aggaccgaac tactcccacg ggcgggtgac ctttttcccc gggcggccgg
     8401 cagcctgcta ttgctgcggc ctgagcggga ggaaacggcg cgaattgctc accacgtggc
     8461 aatcggccgt gcggtcctgc tggacctccg ccgcggcggt cgaacaacgt ccgtacccca
     8521 gcacgccgac catggccgcc gtgattggag ccatgcaggt ggagatcgga ttgcgccgct
     8581 tgctggattc tgcatccgag gcctggtcga tcgagctgac cctcgcgctt ccggtgaagg
     8641 cggaaacgtt tactctgcgc cggagtgagg cctgcccgtt tcacgctccg gaagcttgca
     8701 tgatttctga gcgggacgcc gaagcggtgc gcggcggaaa cagcaggggg ctgacgatcg
     8761 cagatctgct tgcgtcggcg ccggctgttg ccgggacgga gccggttctc ctgctcgatt
     8821 ggccgatctg tgtgcaggcc cggtgcggag cgtgccggga gcggtggccg ccgatgcagc
     8881 gcgtaacggt gatacggcgg cggggcgcct gcccgcattg cggatcgaat caagtgactg
     8941 aggaagaaac gatcagccgc gtgagccgca gcgccccttg ggcgcagctc actcccgccg
     9001 aactggggat tcccgagcag cacttgcata cgatccgctt cgaaagggat cagccgcaat
     9061 gagcggacgc aaagccacga ttgtcgccgc gtcttgtttc ctgttggctg tgctgcaact
     9121 gctcgcggcg ctggtcaatc ccgcggcttc cgtcggcgcc gcagttgttg tcggcgtgct
     9181 tacctttgga atcttgcggc tcttcgacgt ggagctggag cgcgcttggt ctccgcttct
     9241 gatagccgca ggatcctcgg cagcggcgat cggggtgcgc tatttcggca gcccggccac
     9301 agccgaccgg accctggggc tgtctccgct ggttgcgttt gtggcggcgg ccatggttct
     9361 gctcttgcgc cgctcgaaag cccagcgttg cggcctctgc aaccgccgga tcggccggag
     9421 cctggcattc gagtgcccgc gctgcggatt gaccgtttgt gatcagacgt gctggatctt
     9481 cgagcactgc cggtgccggc tgtgcgagca gaatcgcgtg ccggtgtttt cgcccgatgg
     9541 acgctggtgg gaccgccgct tcggcccccg gatgaaacag gggcgctgcc aactgtgcct
     9601 cgccgaggcg gccgacgccg acctgcgcgc ctgcgccaac tgcggccggc cgcagtgccg
     9661 cccttgctgg gattttgcta acggccagtg cagccgctgc cagtggaccg tcgatgacct
     9721 gccggaaggt ttgaaaatgt atatgctgcc gggcgaaccg ggagccgggc gggatgcccc
     9781 ggacaggcgc gccccgattc gatagacgct atggccaagt atcagatgta cttcgacacg
     9841 atcaagggtg agacccgcta caacgtcgat atcgacgagg acgaaactct ggacgccgtg
     9901 ctcgatgaga ttctctacga cctgcgcgag cgcggcgacg ttctccaggg cgacggggaa
     9961 ccccaggtgg tgtggaacgg cacggctttg gactttaccg tcacactgcc cgaacagggt
    10021 gtgcggccga acgacgtcct gcgcgtttcg acggttgcta ccaatgggta acacgcttag
    10081 ccggcaaact tcgccccgcc cgctttcagc gccgggaacc gagaggcccg cggcgccgcg
    10141 cgaccgccgg ctggagcagg agtggaaatt gctaggccag cttgcgcaga ccaaccccgg
    10201 ggtgctcgaa gtgctccggc gccggtcgct ggccgatagc gacattttcg aagcaatgat
    10261 tcatcagaca tgcggcatct tgagcctagc ccgcggcaaa cctgttctcg cgcggtcgca
    10321 tcgcgtcgcg tttcgatttc ctcgcttttt cccctccgtg cccatcgagg ccgttctggc
    10381 caaacctgtc tttcacccca atgtcgatcc gaggaacggg ttcgtgtgtc tttggacgcg
    10441 cacctccgcg gctgacacga cgatggaagc cgcgcggaga ttgcagcaga tgatcgcctg
    10501 gaagttggtc aactttgaag cgacccacgt gatgcagccc gaagccgtcg agtgggcccg
    10561 cagaatgagt tttcccgccg gcctcgcgct ggatttcacg ccgcttgtcg aagtggaatc
    10621 ttttcggcgg gaaaagagct tcacagcccg gcctgacgcg ccgttccgcc gccgtctgga
    10681 accattgcca ggatagaaca gcgccgttaa attccctcgc tgcgcgcgca atggccgacg
    10741 ctggtctcgc cggaaaagac gttgcgctgc acagccgctc aatctgcggc gcaggtgcgg
    10801 aagccggcga aaacatcgcg ccggtctttt gtatagaagt ttcgccaggt attacgaatc
    10861 aggcgcgagc gtgtggccca gcacccgccc cgcagcactt tgcgcccggc gccaaaccaa
    10921 ggctgggaat attctttata aggatccacg acaaatccgg gatagggctg gaagtcgctg
    10981 gccgtccact cccagacgtt gccaatcaat tgccgcacgc ctgccgcgct gtccccggcg
    11041 gctaggtcgc tggcgggaac gcagccgaga gtccgggcat ccagatgcgc ccgctccgcg
    11101 gtcgggcgct catcgcccca aggaaattcc cgctttttgc cgctggacgg cgattcgcat
    11161 gacgccgcca tttcccactc ggcctcggcc ggcagccgcc gcttcgccca ggcgcagtag
    11221 gcctcagcct cgtaccaatt cacgtgaatc accggcagat cctcccccag cgggacgatc
    11281 tggtcgaaat gccggcgcat ccagcgaccg ccggtttggg gaatccagta aaccgggtgt
    11341 tcggcctgcg ccttttcgcg ccaggaccat ccctcctcgc tccagaggcc tcgcctgcgg
    11401 tagccgccgt cgttcacaaa ctcggcgaat tgcctgttgg ttacgggggt gcgagagatg
    11461 gcaaaggact gcactttgac cgcgtgcgcc catttctcgt tatcaaacac aaacgttttg
    11521 cccggcttgg ctcccagttg aaacaagcct ccgggcatga aaacatcccc ctccgcctct
    11581 ggcccgtccc agcccccgcc ggtttcttgc ccaaggccgg ccgtcccttc cagggcaagc
    11641 tccggctccg gataccccag cgtctgccgc gtgtacatga gagcctcgcc gtgcatatct
    11701 tcgtgcatta cggccagcca gtaccagtag aagtaggctt cggagtccgc gacatcgcct
    11761 tggggtaggc tgtcgatgac ccggtcgaag atttcctgca tgtaccgcaa cgtcgcggag
    11821 cggggaagcg tgctccaccg atcatcatgc gcgatcactg cggaatccca gatcgcgtcc
    11881 ccgtccggcc gaatcggatc cttgcccagc aagtgccgca gcacccattt ttcctggaac
    11941 caggccacgt ggccgatctc ccagacgggc ggattgatga tggccagcag aggcactcgc
    12001 atctgcgcct ctgtcagatc gcaaactaac tcgagggttc tgcggcgagc gtcccgcagc
    12061 attgtttcca gttccgttgc ccgcatgctg gtagtattat gcccctaacc gatgcgaatc
    12121 tgcatgtcgt gtccggcgcc acctgggagc ttgtatggca atcgggtaac agccctgcgg
    12181 tggagccgca ttctgcgtga gctggggcac aaagtgtcga tcgtgccggc atacgagaac
    12241 cagccttgcc agttgctcgt ggctttgcac gcccgccgca gcgcggcgtc cgtttttgca
    12301 tttcaagagc agcatccggg aaaaccggtc atcttggctc tcaccggcac cgatgtgtac
    12361 cgggacattc accggttgca gcgagcccga cgcgcgctgg aactggcttc gcggctgatt
    12421 gtgctacagc cgctcgcggt aaacgaatta cccgcaaact tgcggcccaa agcccgggtg
    12481 atttaccaat ctgcggagag aaccgctggc ggccgccgcc ggacggattt tttcgacgtt
    12541 tgcgtagtcg ggcatctgcg cggggtcaaa gatccgttcc gggccgcata tgcgagccga
    12601 ctgcttccaa aggagtcccg catccgcatc ctgcaagccg gggccccgat ggaggaaagg
    12661 atggcgcaga tggcgcgctc cgagcaagtg cgcaatccgc gctaccgatg gctggggggg
    12721 ttgccgcgcg ggcgagcacg ccggctgatt cgctcgagcc gcttggttgt gctgagctcg
    12781 cgcatggaag gcggcgcgaa tgtgatctcg gaagctgttg cggatgggac ccctgtgctg
    12841 gcgtcgcgta tctccggctc ggtggggctg ctgggcgaac gctatcccgg ctattttccc
    12901 gtgggggata cagccgcgct agcggctttg ctccttcggg ccgagtcgga ttgcgccttt
    12961 tatgcgaacc tcacacagtg gtgtttgcgg ctccggccgt tgtttcggcc cgcagcggag
    13021 aaggcggctt gggcaaagct gttgcaagaa gtgtgcccgg cttcccgacc tgactgacaa
    13081 aggctggaca gtcttcggcg ccggcccctc gtgcatgcag ccggcggagg gacggctcgt
    13141 gcggcagccc gtggcccggc aggtctttgt ttggtggcgc cgccgccggt gtgcataagg
    13201 gtcaatataa cgcgggaacc ggggcggcga gatctctcgg gctgagacgg ccgggccgac
    13261 tgcctggaag aaacgctgcg ccgtgcgaga attcggcaag ctggctttgg aggcctctcg
    13321 atgagaaaag acactcatag tgccttgcgg aagcacgtcg tgtatttgtt aaacggcggc
    13381 ggcgctcatt taaccttcga tgacgtcatt cgaaactggc cgcccaa
//
LOCUS       DASREK010000083         5201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1034590, whole genome shotgun sequence.
ACCESSION   DASREK010000083 DASREK010000000
VERSION     DASREK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034590"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFA54_04425"
     CDS             complement(<1..438)
                     /locus_tag="VFA54_04425"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50082.1"
                     /translation="MTPLPPAQTTPAAAQAAPGAPARPAVPAASASGLNITGATLQEV
                     IDILARQLHINYIMDPSIRGTVTINTYGELQINDLMPLLQTILRLNNAVAVQVGNIWH
                     IVPSKNASRLPISPQVNAKDLPEDERMVLNLVALRYVSASDIAK"
     gene            complement(795..1286)
                     /locus_tag="VFA54_04430"
     CDS             complement(795..1286)
                     /locus_tag="VFA54_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50083.1"
                     /translation="MRFLILALLVVLPLAAEGSKSEKPASGPAPAAHAAGAPPAAADK
                     AAAHQTPAPAKAAKEAVPSPVPANSGTVVVQTPFGPTLRAAPDGNAAAPARPGLLADD
                     PMLKIEESGDTVIFRRRTPFGDQVWRRKRSELSDMEREAVEARSKTGLPRPAAPPAAS
                     DHR"
     gene            complement(1397..2158)
                     /locus_tag="VFA54_04435"
     CDS             complement(1397..2158)
                     /locus_tag="VFA54_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50084.1"
                     /translation="MMRRKLLIANIALVALAASGVWRFRAEFRRARERYRVLNPPAAP
                     ALKTQPAEPPPLPPIQPAAYLDAAEKFLFSPDRNPTVVVEPPKVKPRPTLPQLFGVMN
                     LGSGPIAIMAEKANAPHKTVRVGETIGEFKLLGAAGDEITLEWEGQPIHAQVSDVLVR
                     PAADSGGNAPAVAVAGGPGGAAGSGSGGATVMNPNANSLPGEYLIGPPMQGAQGTIYQ
                     SPPGDTAPDGTVYQGKRKVVRQTPFGAQSWWEDVK"
     gene            complement(2155..2742)
                     /gene="gspM"
                     /locus_tag="VFA54_04440"
     CDS             complement(2155..2742)
                     /gene="gspM"
                     /locus_tag="VFA54_04440"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HZS50085.1"
                     /translation="MERLGERDKKALVMLGIAVAAVVVLQFGFPASRNSGEAAVTASI
                     PASERRLQRLQEIARQRPRAAAEAQAASRALADAEKGLLKAATPALASAEMQQIMQEL
                     LRAQGVNMQSSEFGVAKPAGEHYAQVPLTVGFTCGIDQWINLMGAIRNAPQVLSTVDL
                     RIAPGDPKNKLIQVRMVVAGYIPAALLNKAKGTNG"
     gene            complement(2744..4222)
                     /locus_tag="VFA54_04445"
     CDS             complement(2744..4222)
                     /locus_tag="VFA54_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZS50086.1"
                     /translation="MARNPLLTLGAGVGVEIRGNDLEVALVKSRWKGVAVDGKTTLRD
                     FRRRPAAEWGAEYQSFLRSRGFRDLPVTLLLPRQEVIVRLLSLPAPTRAELRSAVRFQ
                     IDTLHPYGEENVYYSFAPLGRRSPNGSAEAGSAAAPALVDVAVVIAERSLIEGYADLF
                     AEAGVKLRAMTVGAAAYWGAVRFLNTRRQEPFLLVDQQGAAFELYGESPARPFFSASF
                     DSAAMPLERAVAAAAAELRLPEGEAVPLLVCGERSIPSPLAPEQVLGSPLQAPAEFDL
                     GRDAAVFATALSAACPLWGWRMNLLPLARRSASSRWPIAATATAAASVGAVALLLFLR
                     GPIQDRRYEQALEREIRRLETVEREVRALEQQAQRARAQRAELESFRRRPEADLALIT
                     EISRRLPKTAWINGIEMNDDSVQLTGQADAAAPLLGLLDSSGVLTGASFTSSITRADN
                     REQFRIRAARRSGPPRPAAAAPPPAGAPAPGHSPAPAAHSGI"
     gene            complement(4379..>5201)
                     /locus_tag="VFA54_04450"
     CDS             complement(4379..>5201)
                     /locus_tag="VFA54_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50087.1"
                     /translation="YYLAEGAIERFVMQLSSSTYVDPKNSPPSVFQAGQRRMRWELPA
                     GVVDLEITGENGKLNVLDAQPAALARLFVLLGIDEGRSRQIAAGIAARRQASRAIESS
                     FSGPTPSFIQLEDLLTVPGVTTDVFYGWWVSPGAPLAPQSVENNSPAAGPAVRLALRG
                     GIASHLTLLDGGAVNVNYASPEVLRAIGVPEGPLMNILQAREGRVIENVASFGAGVLP
                     SGAQLVGGGSEAYTVRATAQLSGRPVRRTVAAVIRYGRDRFEAPLGIIRWYASGN"
BASE COUNT          910 a         1740 c         1652 g          899 t
ORIGIN      
        1 cttggcgata tcagaggccg atacataacg cagcgccacc aggttgagca ccatgcgctc
       61 gtcttcgggc aaatccttcg cgttcacctg gggcgagatc ggcagccgcg aggcgttctt
      121 cgacggaacg atgtgccaga tattgccgac ctgcacggct acggcgttgt tcagccgcaa
      181 aatcgtttgc agcagcggca tcaggtcgtt gatttgcagt tcgccatagg tattgattgt
      241 cacggtcccc cggatgctcg ggtccatgat gtagttgatg tggagctggc gggcgagaat
      301 atcaatgact tcctgcaagg tcgcgcctgt aatgttaagg ccgctggcgg aggctgcggg
      361 cacagcggga cgcgccggcg cacccggagc ggcctgggct gccgccggag tcgtctgcgc
      421 ggggggaagc ggcgtcacgg gtgtgggtgt ctgccccggc gcagcgtgcc ccggcggcaa
      481 attatgttgc ggggcggggg cctgggccgg cgccgggttg ggggctgggg ctgcaccagg
      541 ggcctgccct gccggaagcg gagccttcgg ctgcgctggc ggcgccgcgg aatggccggg
      601 cggcaaattg tgctgtggtg cggccgagtg gcctggcggc agcggcggaa ccgagtgtcc
      661 cggcggaagg ttttgctgct ggaccggagt ggccggagcg acgccagaag ccgggccagc
      721 cgcctgtggc gaggctatga ccggcagcga caaagcaaga agcaaactga gacgtttcac
      781 cctggacatc ctcgttacct gtggtcggaa gccgctggcg gcgcggccgg ccggggcaag
      841 ccggtcttgc ttcgcgcttc gacggcctcc ctttccatat cgctcaattc actgcgcttg
      901 cgccgccaga cctgatcccc gaagggcgtt cggcggcgga agatcaccgt atcgccgctc
      961 tcttcgattt tcagcatcgg atcgtcggcg agcaggccgg gacgggcggg cgcggcggcg
     1021 ttgccatccg gagcagcccg caaggtgggg ccgaaaggag tttggaccac cacggttcca
     1081 ctgttcgccg gaaccgggct ggggacggcc tccttggcgg ccttggcagg cgctggcgtc
     1141 tggtgggcgg ccgccttgtc ggcggcagcg ggcggcgcac cagcggcatg agccgccgga
     1201 gcgggtcccg aagcgggctt ctcgctcttg gaaccctccg ctgcgagcgg tagaactacc
     1261 agcaacgcaa gaatcagaaa cctcattttt tctcctctcg acggctcatc ctccgaaacg
     1321 accactcacg gcgccgcggc gcgtgccttc ttccaaatac tgatcagcga tagctacagg
     1381 gtctggggca tcgtgcttac ttcacgtctt cccaccagga ttgcgctcca aacggggtct
     1441 gccgcacgac ctttcgcttg ccttggtaga cggtcccatc cggcgcagta tcgccgggcg
     1501 gcgattggta aatcgttccc tgcgccccct gcataggcgg cccgatcagg tattccccag
     1561 ggagcgaatt ggcattggga ttcatcaccg tggccccgcc gctgccggag cccgcggccc
     1621 cgcctggccc cccggcgacc gcgaccgccg gagcattgcc gccgctgtca gcagcgggac
     1681 gcacgagaac gtcggaaacc tgagcatgaa tcggctgccc ttcccattcg agcgtaatct
     1741 cgtctccggc ggcccccagc agcttgaact cgccgatggt ctcgccaaca cgaaccgtct
     1801 tgtgcggcgc attggctttc tcggccataa tggcaatcgg cccgctgccg agattcatca
     1861 ctccgaacaa ctggggcaag gttggccggg gcttcacctt cggcggctcg accaccaccg
     1921 tcggattccg atcggggctg aacagaaatt tctcggcggc gtccaagtac gctgcgggct
     1981 gaatgggcgg caaaggcgga ggttcggcag gctgcgtctt cagagcgggt gcggcgggag
     2041 ggttcagcac gcgatagcgt tccctcgccc gccggaactc ggcgcgaaat ctccacaccc
     2101 ccgaggccgc cagagccacg agcgctatat ttgcgatcag gagcttgcgc ctcatcatcc
     2161 gttcgttcct ttggctttgt tcagcaaagc ggccggaatg tagccggcga ccaccatgcg
     2221 cacttgtatc agcttgttct tcgggtctcc gggcgcaatg cgcaagtcca ctgtggaaag
     2281 cacctggggc gcgttccgga tggcgcccat caggttgatc cattgatcga tgccgcaggt
     2341 gaagccgacc gtcaaaggga cctgcgcgta gtgctctcca gccggcttgg caacgccgaa
     2401 ttcggaagat tgcatgttca ccccctgggc gcgcagcagt tcctgcatga tctgctgcat
     2461 ctccgcggat gccagcgccg gggtagcagc ttttaataag cccttctcag catcggccag
     2521 agccctcgac gcggcctgcg cttcggcggc ggcgcgcggc ctttgcctcg cgatttcctg
     2581 caagcgctgc aaacgcctct cgctagccgg aatggaagcc gttaccgccg cttccccgct
     2641 gttccgagaa gctggaaagc caaattgcaa cacgaccacg gcggccacgg cgatccccag
     2701 catcaccaga gctttcttat cgcgttcgcc gaggcgttcc atttcagatc cccgagtgag
     2761 ccgcaggagc gggcgaatgg ccgggcgcgg gcgcccccgc cggaggcgga gcagccgccg
     2821 ctgggcgtgg cgggccgctg cggcgcgccg cgcgaattcg aaattgttcc cggttgtcgg
     2881 cccgggtgat cgaactcgtg aacgaagctc cggtcaacac gccggagcta tcgagcaacc
     2941 ctagcagcgg cgccgccgca tcggcctggc cggtcaattg cacgctgtcg tcattcatct
     3001 cgatgccatt gatccaggcc gtctttggca ggcggcggga aatttccgtt atgagcgcca
     3061 agtccgcctc gggccggcgg cgaaagcttt ccagttccgc tcgctgcgcc cgcgctcttt
     3121 gcgcctgctg ttcgagcgct ctcacttccc gctccaccgt ttccagacgc ctgatttccc
     3181 gctccagcgc ctgctcgtac cgcctgtcct ggatcgggcc ccgcagaaaa agcagcagcg
     3241 ctacagcgcc gaccgaagcc gcggccgtag ccgtagcggc aatcggccag cgggagcttg
     3301 cgctccggcg cgccagcggc agcaaattca ttcgccagcc ccagagcggg caagcggcgc
     3361 tcaaggctgt cgcgaagacc gcggcgtcgc ggcccagatc gaattccgcc ggagcctgca
     3421 aagggcttcc caacacctgt tcgggcgcca agggcgatgg aatcgaacgc tcgccgcaaa
     3481 ccagcagcgg cacggcttcg ccctcaggga gccgcagctc cgccgccgca gcggccactg
     3541 ctctttcgag aggcatcgcg gccgaatcga agctagcgct gaagaacgga cgcgccgggc
     3601 tctctccgta cagttcgaag gccgcaccct gctgatccac gagcagaaac ggctcctgcc
     3661 ggcgcgtgtt caggaaccgt acagctcccc aataagccgc tgcgcccacc gtcatggccc
     3721 gcagtttcac tcccgcttcc gcgaacagat cggcgtagcc ttctatcagt gagcgttccg
     3781 cgatcacgac ggcaacgtcc accaacgcag gagcggccgc gctccccgcc tccgcggagc
     3841 cattcgggga acgccgccca agcggcgcga agctgtagta gacattctct tcgccgtagg
     3901 gatgcagagt gtcgatctgg aatcgcacgg cggagcgcag ttcggcgcga gtcggagccg
     3961 gaaggctgag cagccggacg atcacttcct gccgcggaag cagcaacgta acaggcaggt
     4021 cgcggaatcc gcggctcctt agaaacgact ggtattcagc cccccattca gcggccgggc
     4081 ggcggcgaaa gtcgcgcagc gttgttttcc catccacggc gacgcccttc cagcgcgact
     4141 tgaccaaagc tacttcgaga tcattgccgc ggatctcgac gcccacgccg gcgcccaagg
     4201 taagaagagg atttcgagcc atctcagttc ctattcaccc ccaacaatcg ccgaccagtt
     4261 ccgacattcg ccggcaaagc gcctttccat cgttcctgct ctgccgcctc gcgcggcgcg
     4321 aagctgcggg gaacccaggc gcccggcctg ccttcttccc gcgagccgcg gattcccgct
     4381 agtttcccga tgcataccac cgaatgatac ctagaggcgc ttcaaagcga tcccggccgt
     4441 aacgaatcac ggcggccact gtgcgccgaa ccggccgccc gctcaattgc gccgtggcgc
     4501 gcaccgtgta agcctcagag cctccgccca ccaactgcgc tccgctgggc aaaacaccgg
     4561 cgccaaagga cgcgacattc tcgatgacgc gcccttcgcg ggcttgcaga atattcatca
     4621 ggggcccttc gggaactcct atcgcccgca gcacttccgg ggaggcgtag ttcacgttta
     4681 ccgcgccgcc gtcgagcagc gtcaggtgcg aagcgatccc gccgcgcagc gcgagacgta
     4741 ctgcgggtcc ggccgcggga gaattgtttt caacgctctg cggcgcgaga ggcgcgcccg
     4801 gcgataccca ccagccgtaa aaaacgtcgg tcgtcacgcc gggaacggtc aaaagatcct
     4861 ccaactgtat gaaggacggc gttgggccgg aaaaagacga ttcaatcgcg cgactcgcct
     4921 gccgccgggc tgcgattcca gccgcaattt gccgggaacg gccctcatca attccaagaa
     4981 gcacaaaaag ccgcgccagg gcggcaggct gggcgtccag aacgttcagc tttccgttct
     5041 cgccggtgat ttccaggtcc acaacgccgg ccgggagttc ccagcgcatc cgcctttgcc
     5101 cggcctggaa tacagaggga ggagaattct taggatccac gtaagtcgag ctggacaact
     5161 gcatgacaaa gcgctcgatt gctccctcgg cgagatagta g
//
LOCUS       DASREK010000084        19112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1369484, whole genome shotgun sequence.
ACCESSION   DASREK010000084 DASREK010000000
VERSION     DASREK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 19112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19112
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369484"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2397)
                     /locus_tag="VFA54_04455"
     CDS             complement(<1..2397)
                     /locus_tag="VFA54_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50088.1"
                     /translation="MRLTLLSCLLGLMGWATQPGVGLAQDVRAQAERLERLGDVVRSQ
                     ALLAKAAESNPKDAAAQQAYAQFLDRYHDPLRRTAYQRWLDAVNPADHAARAEITRRL
                     ALLDLIEGDQRAAAAHLAEYQAAGGRAAPEILSALRSEPPGEQLPSQSETEIPGPLRS
                     FARMAALSTDLAPADVLPALARNVITNGYQASRSNEALEPTEYLKLVNRYLSQARELT
                     QLAGKDQVISVPACESEQTGQLLRILGYRLRSGCGDDAALETVNASRAFLTIDSGFPL
                     ADLEDSLRRNQPFRYVYKPAKIPVMFGVGYWTSNQKSQQGVDFLDSFIGDPALARLYL
                     AFSKLDPVTAEALRKAYPLERWKAYAHVLDFFGSRFELRNGRAVTPGGERSAAAWGEM
                     VGASPEKGPEFFERLISKDDGWMASFFDALSRTRGPLRDYLTEPARMRRFYAAVRGRI
                     TSPGPARPVFRSNADLMLLTSRLQIQPDGTPHIPGGLDPWKQLFATHPHGKYDGKLTR
                     AAAGWRTPDDVVEALFALCRKSVENEPLKIFMAISDIDRNRRSPLRPATVSRLTVDYR
                     PYGAQYAVFNDAPALTDETILSFLDTIAALSKIKDPGQRANTAGMFQGLVSLWQIFCR
                     QDQIPAAKADAALKSLIGLFGSIKNDRELFDAGRSGVRVLLAATNSPEDASPQDQVVA
                     LLAGTPRPGDREIQRQVASDLNSLFEAQRLVSLKTLFDLADHLESVSRGERLNVAMAN
                     RLASRIAEIRLPRSTLTTAEKNSFAFGYWTEKHIENQRSLNLRQIVDKAAGQPEKLKD
                     V"
     gene            complement(2532..4364)
                     /gene="uvrC"
                     /locus_tag="VFA54_04460"
     CDS             complement(2532..4364)
                     /gene="uvrC"
                     /locus_tag="VFA54_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZS50089.1"
                     /translation="MVPPESLEFLKQKAGELPASPGVYLYKDAAGKVIYVGKAKSLRD
                     RVRNYFSDERLADAKTGTLVSEARDLDYILVDNEKEALALENNLIKKWQPRFNVLLRD
                     DKTYPYIKLTISEKYPRVYVTRRLKKDGSVYFGPYFPANLAHRLVHFIHRYFRVPSCK
                     VDLARFHPRPCLQYHIHRCLGPCVQGLTTDEIYAQAVHQVKMFLEGRHEALAKELRAR
                     MEQAAANLEFEQAAALRDLIATVEELDERQKVAAAEGQDIDIFGYYAEPPLVAANLFH
                     LRNGRIVDRRELFWEDQEEFNPREFFSSLLKQIYIGRQYVPAQIHVPVEFEDREALAE
                     YLTEMRGRKVEIVTPQRGQKRAMLELVERNAKHSFEQRFRVLKPSSKQIIGALEDALN
                     LPEITPRIECFDVSHTQGTDTVASMVVWENGRMKKSDYRKFIIRTVDGNDDFASMREV
                     VARRYARLQQEGKPLPGLVLIDGGLGQLHAAAEALEAVGVINQPLVSIAKKEEIIYVY
                     GQENEPVVLDRYSPVLHLVQQLRDEAHRFAVTFHRSRRGAARLASELGQIPGVGERTL
                     RKLLKTFGSLEGVRQAGSDQLASVVGAAQAGRIAGYFAGRSGGT"
     gene            complement(4576..7881)
                     /locus_tag="VFA54_04465"
     CDS             complement(4576..7881)
                     /locus_tag="VFA54_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS50090.1"
                     /translation="MKIQRPFLLVSLCLISTLPALAQTATGEVSGTVTDASGGVVASA
                     KVTVTNSETGTSREGSTDSSGAYIFTLLQPGFYNLSVEAPGFRKTVRNGVELRVNQRA
                     EVNLQLQVGQVTDTVEVAASAPLLESQSSTLGSVIDTQLTEELPLNGRNFVQLATLSP
                     GVNGTGYSVAGTIMSGTRPDDRRPGTEIFSNGNREGSNDFLYDGIDNNDRLTLSIVLR
                     PGVEAIKEFKVQTNLYSADQGRNSGAVVDVVTKSGTNQWHGSVFEFLRNSAMDARNFF
                     NTKGTPFPSFRYNQFGGSFGGPVEIPKIYKGRNRTFFFVDYEGFRRNSQQLLTTTVPT
                     AAMRQGDFSALPVKIYDPTTTTPSGSSYTRTQFPGNMIPASRFDPITAKLVNAYPLPQ
                     RSALVSNYVSNMTQQQNWDQGDIRVDHQFSSSDTFFARYSIQKTDTIVPSTFPPTTVA
                     GIPHPVSLGNEDSFAGTSSTPVQQTVASYTHIFGPRVINDLRAGFNRFRVDYTLQGTT
                     PTEKLGEELGVANANPNAQQTGVPIVSPSNYAGIGQSRSLPIYRRENTFEILDNVTWT
                     RGAHTVKFGLDVRRRQITEYQTNRGNGRFNFSTGFTAQPGVNSGDSIASLLLGYPTLY
                     EQDYLLVWPGIRGTEAGAYVADDWRISKRLTLNVGLRWEYYSPYSEVANRWANFDFAS
                     ATIKVAGQNGFSSTAGVQGDWRDFAPRFGFAYQALAHTVVRGGFGLFYNPNGNGGALL
                     RLDRQAPYGPILSISPGDQTLGPRVSDGFPPAPSVNFSALSNPTGNVIGIPSNLKQAY
                     AEQFNLTVEQELAPVNTLLKFAYVGNLGRRLGNSYNPNQPVPGPGPTAPRRLFYSIRP
                     GLGDITYYVSDGLSNYNAFQLTVEKRLSSGISGLLGYTWAHAIDNVATDFGGGTGTPQ
                     DPRNRNLDRGNSAYDIRHRFTASFTYRLPGFAWKGLTGALLGGWQTNGILQWQTGLPF
                     TPQLNNPTVNTGTGSRPDRIGSGNLPSGQRSITHWFDPSAFATPAQFVYGNAGRDILF
                     GPGRANLDLSLFKNFRPAERLTVQFRAESFNVFNHPQFGQPNASIGSNSVATITSTVG
                     NPRQMQLALRLVF"
     gene            8023..8454
                     /locus_tag="VFA54_04470"
     CDS             8023..8454
                     /locus_tag="VFA54_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50091.1"
                     /translation="MHRHWVLACLLCFASSAGLAQRSRQTGPPPAPPEPDQEQENGEP
                     EYTFNPIQAQKELKIGDFYFKKKSYRAAAGRYERATKWQPDLAEAYYKLGESREKLDQ
                     NEQALAAYQKYLDLSPPPKQAEEARKKIAQLQKQRSATASK"
     gene            8451..10730
                     /locus_tag="VFA54_04475"
     CDS             8451..10730
                     /locus_tag="VFA54_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50092.1"
                     /translation="MSSPPPAPDLFDRRPILILILLTIGFFWRLVLSRQFTFLESPDL
                     AYQVLPWYQVEARAFHHGVFPMWDPYQWSGQSLLGQMQPGAAFPLNWLLFLMPLRDGH
                     LNFVWVHRHFVLMHILAALFMYAFCRRLGSSRFASLMAGAAFALGGYMGTTLWPQMLE
                     GAMWIPLVFLFFHRMVEQAQKGSSRLPALSSAALCGGTIGVSLLSGHHQTPMFTLLAL
                     CGVFLYFLCARPGLRLRLLTLFGAVGVFAFLVSALQLLPAAEYGMHAYRWVGTENPVR
                     MNEPVPYLAAETGGLYPISLLGVVFPRAFLTTDPFLGFVCLSFALFAVFTNWRLARVR
                     IYSVLALAAVAYASGIYSVLHGLIYALVPFADKARSPGHAIFVFQFAAFTLTAQGIDC
                     FFSPQEPEWERWRHRIIKALAAAGSLAWIAMFWLYMNMKFETKPGDHVALASMVAFVL
                     AGVLYGFGRGRLSAAGARAALIALLLFEMGIASYWIIPHRSDPLRAQYLHHLTDHAGI
                     VQFLKAQPGPFRFDLPPGNDFPANMGDWEGLEGTRGYLASVSAGLFDFMSWDWTQTPL
                     LLNTVYVVSKTPTRPNQREVFADSDGWKVFRNEDALPRAWVVQSVRMAPSAREAAAIF
                     GAPGFDPRRETVLLASRSEKPPLEPCTGEAAVEVTGVTFHHVAALARTPCRAMVVFGD
                     PYFPGWRARVDGSPAALYAPFGALRGVIVPPGTHKVELVYRPRSVLIGALLSALGFIG
                     CACLAWLARSRSSQRTASR"
     gene            complement(10613..11464)
                     /locus_tag="VFA54_04480"
     CDS             complement(10613..11464)
                     /locus_tag="VFA54_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZS50093.1"
                     /translation="MRALVLSAGGTFGAYHAGAWQALEEAGFRPDIVAGASVGALEAA
                     LIARGCTAAQLSAWWRDPRWNVFRWNWPPRVWGLLDPRRLEKRVTELCERFSKPLPGV
                     KLLVTLTELPGAKICVKADEEVTAPCLLASCAIPLFYPPVKIDGRWYCDGGVFCRLPL
                     GAAAAAGAAEIVAIDLLAAPPSRVLRAALGAACAVREAAIRQRDVSQIANPGVRVLRI
                     EPSRPLGRPRDMLRWNPGQINQWIEAGYRDAVRWLDRERASQAKHAQPINPRAESSAP
                     IKTERGR"
     gene            11533..12207
                     /locus_tag="VFA54_04485"
     CDS             11533..12207
                     /locus_tag="VFA54_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HZS50094.1"
                     /translation="MPDAFPAHEETGLLVEVRNGPGVLHQLTGVIFRHGGDITSVAIL
                     DSDKPLASVFFEIAGVDAVAAIEEDLQALDVVASVGRTTTLQKVYGKRLIIMGGGAQV
                     GQVAIGAISEADRHNIRGERISVDTIPLVGEKALADAVRAVVRLPRAKALVLAGSLMG
                     GEIEAAVREVRQKGLLVISLNMAGSVPEAADLVVSDPVQAGVMAVMAIADTAAFNVAR
                     LKTRVF"
     gene            12424..13755
                     /locus_tag="VFA54_04490"
     CDS             12424..13755
                     /locus_tag="VFA54_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50095.1"
                     /translation="MSAAQSAAVGLNFEEDLYIPPVPQTVEETGLAPSHIEQLIIKTL
                     YFNGETVGRDLANALGLKFSLIDGIMEFLKRTRLVEVKRSSGFGNVSAVFALSEAGRI
                     RGREYLDINQYAGRAPVPVEQYVEVVRKQRAKDGWLTKETLTLAFQHMVVSPETITRI
                     GPAVNSGKSFLLYGQPGNGKTYMAEALQNVEHSCIYVPYALEVQGQIIQMFDALYHRR
                     VDEVDASQSSLAVDPLYDGRWARCRRPFIVTGGELTLEMLDLSYNPSAKIYDAPFQLK
                     ANNGIYLIDDFGRQKVSPAELLNRWIIPMDRRVDYLTFHTGGKVAVPFEAFLIFSTNL
                     HPDTLGDEAFLRRIQYKMYLKSPTQEEFIEIFKRYCESENLYCPSTVLDRFIEKHYRR
                     TGKRFRRCHPRDIITHAIDLIRFEKAPYELTEQMLDRAFESCFLESSEIVD"
     gene            13880..14281
                     /locus_tag="VFA54_04495"
     CDS             13880..14281
                     /locus_tag="VFA54_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50096.1"
                     /translation="MLGEMLGESRGKRTGRRVLPMDGPGVKVEVAFEDSGKLLGIDMN
                     NIGTYWSATRPDGTLYGEGQGVIVTRDGDMGTWKGAGIGQLKGGAVSYRGAIFYYSAS
                     PKLARLNSIAAVFEFAVDPEGNTHTKLWEWK"
     gene            complement(14298..15158)
                     /gene="mutM"
                     /locus_tag="VFA54_04500"
     CDS             complement(14298..15158)
                     /gene="mutM"
                     /locus_tag="VFA54_04500"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZS50097.1"
                     /translation="MPELPEVETIVRGLASRLPNRRIERIEVFQPRVIIGDIQRAEGF
                     TIAQVRRRGKFVLLDLERGGRRGHLLAHLGMTGQLRLAAEPGPYTRVLFQLDKGALLY
                     NDVRQFGRLEFAERLPARFKRLGSEPLEIGEEEFVRRFRARRAMVKPLLLNQAFLSGL
                     GNIYVDEALFRARIHPRALANRIRPGRAAKLYRAIVRVLREAIAHRGSSISNYVGSDG
                     RRGAFQHLHKVYRRTGLPCPVCGAAIRRILVSQRGTHFCPRCQKIRSGAGRAPEPIGG
                     TLGSARRGAR"
     gene            complement(15160..16023)
                     /locus_tag="VFA54_04505"
     CDS             complement(15160..16023)
                     /locus_tag="VFA54_04505"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HZS50098.1"
                     /translation="MKILVIGGTGTVGSQTARNLVGRGESVRIMTRSAEKAKAVPRGA
                     EAVTGDLDAPASLPGAFRGIDAVFLATPLSPTETQAGLAAVAAARSAGVRRFVYMSVH
                     NLERAPHIPHFKSKLPIEKSVRESGLEHTILAPNNFFQNDLLMREAIVRWGVYPQPIG
                     NVGLNRVDVRDIADAAARALTEAGHNGKKYPLVGPDLLTGESTARIYSRRLGREVRYA
                     GDNLDSWAEQMRRMLPDWLVRDLRIMYEYFQAHGLAATKEDLDQQQAILGHAPRNFDE
                     FVAGLAPEWRA"
     gene            complement(16128..16490)
                     /locus_tag="VFA54_04510"
     CDS             complement(16128..16490)
                     /locus_tag="VFA54_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS50099.1"
                     /translation="MFKVEQIDHIALTVADVERSVAWYRDVLGLERRYQEAWGSFPAV
                     MCAGSTAIALFPAASTEPGHLPRAKRPTVIRHLAFRTNRANFEEAQVDLKSEISHSTL
                     KTTIFVTQSISGTRTAMR"
     gene            complement(16591..17157)
                     /gene="nuoB"
                     /locus_tag="VFA54_04515"
     CDS             complement(16591..17157)
                     /gene="nuoB"
                     /locus_tag="VFA54_04515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HZS50100.1"
                     /translation="MAFIQNQFEKNFLTTSVDYVFNWARKSAIWPLTFGLACCAIEMI
                     ASSTSRFDIARFGSEVFRPSPRQSDLMIVAGTVTLKMAPVLKRIWDQMPDPKWCISMG
                     ACSSVGGPFNTYAVLQGVDRIVPVDVYITGCPPRPENLFYGLLKLQDKIDQMTTLVKR
                     PTEVRLDESMLEQFKKRVMVAQVQNPAA"
     gene            complement(17165..17911)
                     /locus_tag="VFA54_04520"
     CDS             complement(17165..17911)
                     /locus_tag="VFA54_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS50101.1"
                     /translation="MTEAEEALLRAEFDRRALANPRLEYRHPAIMEKVFDRMQVARAE
                     RSLDIGCGSGWATRRLAQMASEGLAVGMDISDEMIRLARRLSVALENCMFVSGAADEI
                     PWQEDFFSLALSVDSAFYWPDPAAAASEIRRILSPGGRLVLLLSYFRENPMHKRWQGL
                     FRVPVHLKAGAEWAELFAAIGFEAVRHEQITDESPVEPGFAPSEFYPTPEDKLAFRRA
                     GALLIEARKPVLRPGSQPPAAPHTPLVVLE"
     gene            complement(17904..>19112)
                     /gene="thiC"
                     /locus_tag="VFA54_04525"
     CDS             complement(17904..>19112)
                     /gene="thiC"
                     /locus_tag="VFA54_04525"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZS50102.1"
                     /translation="NLEPMCIGVASACKVNANIGNSAVTSDVDGELEKLRYAIKYGAD
                     TVMDLSTGGDIPNIRKHIIAHSPVPIGTVPIYEALTRVRRVQDLTPQLMLEVIEEQAE
                     QGVDYMTIHAGVLIQYVPLAVKRITGIVSRGGSILAEWMVKNHKQNMLYEHFDEICKI
                     FQKYDVSFSLGDGLRPGCLADASDEAQFAELKTLGELTKKAWEYDVQVMIEGPGHIPM
                     DQIQMQVEKEREWCYEAPFYTLGPLVTDFAPGYDHITSAIGAAMIGWHGASMLCYVTP
                     KEHLGLPNKEDVKAGLIAYKIAAHAADIARHRPGARDRDDALSHARYNFDWNRQFELS
                     LDPETARALHDETLPQEGFKEAHFCSMCGPRFCSMNISSTVEGFTAEEAEAVAAGLAR
                     PQLVTIAGND"
BASE COUNT         3554 a         5847 c         5921 g         3790 t
ORIGIN      
        1 aacatccttc aacttttcgg gctggccggc cgccttatcc acaatctggc gcaagttcaa
       61 gctgcgctgg ttttcgatgt gtttctcggt ccagtacccg aaagcgaacg aattcttctc
      121 cgcggttgtc aacgtgctgc gcgggagccg gatctccgcg atccgggagg ccagtcgatt
      181 ggccatagcg acattgagcc tctcgcctcg cgacacgctc tccaggtggt ccgccaaatc
      241 gaaaagggtc ttcaacgaca ccagtctttg ggcttcaaac agggagttga gatcggaggc
      301 gacctgccgc tggatctcgc gatcgcccgg ccgcggcgtt cccgcgagca gcgcgaccac
      361 ctgatcttgc ggcgacgcgt cctcgggcga attcgtggca gccaatagca cgcgcacgcc
      421 cgaccggccg gcgtcgaata gttcccggtc attctttatc gatccgaaca ggccgatcag
      481 gcttttgagg gcggcgtccg ctttcgccgc agggatctga tcctggcggc aaaagatttg
      541 ccacagactc accagcccct ggaacatgcc cgccgtattc gcacgctggc cgggatcctt
      601 gattttgctg agcgcggcga tggtatcgag gaacgacaga atggtctcat cagtcaaagc
      661 gggagcatca ttgaacacgg cgtattgggc gccgtaaggc cggtaatcca cggtcagccg
      721 cgagaccgtc gccggccgca gcggggagcg ccggttgcgg tcgatgtcgc tgatggccat
      781 gaaaatcttg agcggctcgt tctccaccga cttgcggcag agagcgaaca gcgcctccac
      841 cacatcgtcg ggcgttctcc aacccgccgc cgcccgggtg agtttcccgt catactttcc
      901 gtgcggatgc gtggcgaaaa gctgcttcca aggatccaga ccgccgggaa tatgcggcgt
      961 tccgtcgggt tgaatctgaa gccgcgaagt aagcagcatc aaatcggcat tcgaccggaa
     1021 gaccgggcgg gcgggtcccg ggctggtgat acgcccacgc acggccgcat agaagcgcct
     1081 catacgcgcc ggctcggtca gataatcgcg aagcggcccc cgggtgcggg agagggcgtc
     1141 gaaaaagctg gccatccagc cgtcgtcttt ggatatgagc ctctcaaaga actccggtcc
     1201 tttctccggg gaggcgccca ccatttcccc ccaggcggcc gcgctccgct ctccgccggg
     1261 agtcaccgcc cggccgttcc gcagctcgaa gcggctgccg aagaaatcca gtacatgagc
     1321 ataggctttc catctctcga gcggatacgc tttgcgcagc gcctcggccg ttaccggatc
     1381 gagcttgctg aaagccagat acaaccgcgc cagtgcgggg tcgccaatga aagaatccag
     1441 gaaatcgacg ccttgctgcg acttttgatt ggaggtccag tagccgaccc cgaacatcac
     1501 agggattttc gccggcttgt acacatagcg gaagggctga ttccgccgga gcgaatcttc
     1561 caggtcggcc agtggaaaac cggaatcgat cgtcaggaag gcgcgcgacg cgttcactgt
     1621 ttcgagcgcc gcgtcatccc cacacccgct ccggagccgg tagccgagaa tgcgcagtag
     1681 ttgcccggtt tgctccgatt cgcaggctgg aacgctgatg acctgatctt tcccggcaag
     1741 ctgcgtcaat tcccgcgctt gggaaaggta gcgatttacg agtttcaaat attcggtcgg
     1801 ctcgagggcc tcgttgctgc gggaggcctg atagccgttc gtgatgacat tgcgagccag
     1861 ggcgggcaac acatcggcgg gggctaaatc cgtggaaagg gccgccatgc gggcaaacga
     1921 gcgcagcggc ccgggaattt ccgtctccga ttgggatggc agttgctcgc ccggcggctc
     1981 gctccgcaaa gccgatagaa tttccggagc tgctcttccg cccgccgcct ggtactcggc
     2041 cagatgcgcc gcggccgccc gctggtcgcc ttcgatcaaa tcgagtaaag ccaggcgccg
     2101 cgtgatttcc gcccgggctg cgtgatcagc cgggttgacg gcatccagcc agcgctgata
     2161 tgccgttcgg cgcagcgggt cgtggtatcg gtccaaaaac tgagcgtagg cttgctgtgc
     2221 cgccgcgtcc ttcggattgg attcggctgc cttcgcgagg agcgcctggg accggacaac
     2281 gtcacccaag cgttccagcc tttccgcctg cgccctcaca tcctgagcca gcccaacccc
     2341 cggctgggtg gcccacccca tgagaccgag taaacagctc agtagtgtaa gccgcatgac
     2401 tgttctcccc gccaaaaaga acgtggcttt ttggtttatt gattctatgg cagcaggatc
     2461 atcaagacaa ctctgactcg aaatttcacc gaggccgcag caaagtttac acgccggacg
     2521 ggaagcaagc cttacgttcc gccggaccgc ccggcgaagt atccggcgat ccggccggct
     2581 tgcgccgcgc ccactacgga agccaactgg tcgctgccgg cttgccggac gccctctaga
     2641 cttccgaatg ttttcaatag cttgcgcaac gttcgttcgc ccactccggg gatttgcccg
     2701 agctcggaag ccaagcgcgc cgcgccccgg cggctgcggt gaaacgtcac cgcaaaccta
     2761 tgagcctcgt cccgcaactg ctggaccaaa tgcagaaccg gagaatagcg gtccagcacc
     2821 accggctcgt tctcttgccc gtaaacgtag atgatttctt ctttcttcgc gatagagacc
     2881 agcggctgat tgatcacgcc caccgcttcc agcgcctccg ctgcggcgtg caattgcccc
     2941 aagccgccgt caatcaaaac cagcccgggc agcggcttgc cttcctgctg caagcgcgcg
     3001 taccgccgtg ccaccacctc tcgcatagag gcgaaatcgt cgttgccatc gaccgtgcgg
     3061 ataatgaact ttctgtagtc gctctttttc attctgccgt tttcccacac caccatggaa
     3121 gctaccgtgt ccgttccctg ggtgtgggaa acgtcgaagc attcgatgcg cggggtgatt
     3181 tccggcaggt tgagcgcgtc ctccagcgcg ccgatgattt gctttgagct cggcttcaga
     3241 acgcggaagc gctgctcgaa gctgtgcttc gcgttgcgct ccaccagttc gagcatggcg
     3301 cgcttttgcc cgcgctgcgg cgtcacgatc tccactttcc ggccgcgcat ttcggtcaaa
     3361 tactccgcca gcgcctcgcg atcttcgaat tccaccggca cgtgaatctg cgcagggaca
     3421 tattggcggc cgatgtagat ctgcttcaga agagaagaaa agaattcgcg gggattaaac
     3481 tcctcctgat cctcccagaa gagctcccgg cggtccacga tgcgaccgtt cctcaggtga
     3541 aacaggttcg ccgcgaccag gggcggctcg gcgtaatagc cgaaaatgtc aatatcctgg
     3601 ccctcggcgg cggccacttt ttgccgttcg tccagttcct ccacggtcgc gatcagatcg
     3661 cgcagcgcgg ccgcttgctc gaactccagg ttggcggcgg cctgctccat gcgcgcacgc
     3721 aactctttgg cgagggcctc gtggcgtcct tccaaaaaca tcttcacttg atgcaccgct
     3781 tgggcgtaaa tctcgtccgt ggtcagtccc tgcacgcagg gccccaggca gcgatgaatg
     3841 tggtattgca gacagggacg gggatggaaa cgcgcgaggt ccaccttgca ggaaggcacg
     3901 cggaagtaac gatggatgaa gtgcacgagc cggtgcgcca ggttggccgg gaaataagga
     3961 ccgaagtaga ccgaaccgtc ctttttcaaa cgccgggtta cgtacacccg gggatatttt
     4021 tccgaaatcg tgagcttgat gtacgggtag gtcttgtcgt cccgcagcag cacgttgaaa
     4081 cgcggctgcc attttttgat caggttgttt tcgagggcca gagcctcttt ttcattgtca
     4141 acgagaatgt agtcgaggtc gcgggcctcc gacacgagtg tgccggtctt ggcgtcagcc
     4201 agccgctcgt cggaaaagta gttccggacg cggtcccgca ggctcttggc cttgcccaca
     4261 tagatgactt ttccggccgc atccttgtag agatacacgc ccggcgacgc tgggagttcg
     4321 ccggccttct gcttcaaaaa ttccaggctc tcgggaggga ccatagcagg tctttccctt
     4381 gattttagcg cgataacccc ttccagccgc gaggccgaag ggcgcgccgg agcagacagt
     4441 ctgttcggca agcggttgcg ggaaatgcgt ccggatcgcc gccgcggccg ccgaaatcga
     4501 gctctgagcc gcgcggtaat acagcgggca gcaaggcgag cttagcttaa gacttgcttg
     4561 acacggtcgc ccgcctcaga agaccagccg caaagccagt tgcatctggc gcggattgcc
     4621 gacggtggac gtaatcgtgg cgaccgagtt gcttccaatc gaggcattcg gctgcccgaa
     4681 ctgcggatgg ttaaagacgt tgaacgattc cgcgcggaac tgtacagtca ggcgttcggc
     4741 cggccggaaa tttttgaaca gcgacaagtc caggtttgcg cggcccgggc caaagagaat
     4801 gtcacggccc gcattgccgt aaacgaactg cgccggcgtg gcgaaggcgg acgggtcgaa
     4861 ccagtgggtg atcgatcgct ggccactcgg gagattgccg ctgccaatgc gatcgggacg
     4921 gctgccggtt ccggtattga cagtggggtt gttcagctgg ggcgtgaacg gaagcccggt
     4981 ctgccattgg aggatcccat tggtctgcca tccgcccagc agcgcgccag tcaagccttt
     5041 ccacgcaaaa cccggaagcc ggtacgtgaa gctggccgta aaccggtggc ggatgtcgta
     5101 ggccgagtta ccccggtcga gattgcggtt tctcgggtcc tgcggcgtac ccgtgccccc
     5161 gccaaaatcg gttgccacgt tgtcgatcgc atgggcccag gtgtagccta gcaggccaga
     5221 aattccggag gagagccgct tctcaacagt gagttgaaat gcgttgtagt tgctcagccc
     5281 gtcggatacg tagtaggtga tgtcgcccag cccgggccgg atgctgtaga acagccggcg
     5341 aggcgcggtc ggccccggcc ccggcacggg ctggttaggg ttgtagctgt tccccaaccg
     5401 ccggccaaga tttcccacat aggcgaattt caacaatgta ttcacggggg ccagttcctg
     5461 ctcgacggtg agattgaact gttcggcgta tgcttgcttc aaattgctcg gaatcccaat
     5521 gacgttgccg gttggattgc ttaaggcgct gaagtttacg gagggagccg gcgggaatcc
     5581 atcgctgacg cgcggtccga gagtctgatc gcccgggctg atcgagagaa tagggccata
     5641 gggggcctgg cggtccaggc gaagcaaggc gccgccgttg ccgttggggt tgtagaaaag
     5701 tccaaagccg ccgcggacca ctgtatgggc gagggcctga taagcgaagc cgaagcgcgg
     5761 ggcgaaatcc ctccagtccc cctgaacgcc ggccgtcgag ctgaatccat tttggcctgc
     5821 cactttgatc gtggcgctcg caaagtcgaa attcgcccat cggttggcga cttcgctata
     5881 cgggctgtag tactcccacc gtagcccaac atttagcgtg agcctcttgc ttatccgcca
     5941 gtcgtccgcc acataagcgc cggcttccgt tccccggatg ccgggccaga ccagcaggta
     6001 gtcctgttca tatagtgttg gataacccag cagcaggctg gcgatggaat cgccgctgtt
     6061 caccccgggc tgggcagtga agccggtgct gaagttaaag cgcccattgc cgcggttggt
     6121 ctgatactcc gtaatctgcc gccggcggac gtccaggccg aacttgacgg tgtgagcgcc
     6181 gcgggtccag gtaacgttgt cgagaatttc gaaggtgttt tcgcgccggt agatcggcag
     6241 cgagcggctc tgtccgatgc ccgcgtagtt ggagggggag acgatcggca ctccggtttg
     6301 ctgcgcgttg ggattggcgt ttgcgacacc gagttcctcg ccgagcttct ctgtaggggt
     6361 cgtgccctgc aaagtgtagt ctacgcggaa gcggttgaat ccggcgcgca ggtcattgat
     6421 cacccgcggt ccgaatatgt gggtatagct tgccaccgtc tgctgtacag gagtcgagga
     6481 tgtgcctgcg aatgaatcct cgttgccgag cgataccgga tgaggaatgc cggcgactgt
     6541 ggtgggagga aacgtgctgg gaacgatggt gtcggttttt tgaatcgaat agcgcgcgaa
     6601 gaaagtgtcg ctggagctga actggtgatc gacgcggata tcgccctggt cccagttctg
     6661 ctgctgcgtc atgttgctga catagttgct gacgagcgcg ctgcgctgcg gcaagggata
     6721 agcgttgacc agcttcgcgg tgatcggatc gaagcggctg gcaggaatca tgttgcctgg
     6781 aaactgcgtg cgcgtgtacg aggagcccga cggcgtcgtg gtcgttggat catagatctt
     6841 taccggcaac gcgctgaaat cgccttgccg catggctgcc gtaggaactg tagtggtgag
     6901 gagctgctgg gaattgcgcc gaaacccctc ataatccaca aaaaagaacg ttctgttcct
     6961 gcccttgtag atcttcggga tctcgaccgg gccgccgaaa gagccgccga actggttata
     7021 gcggaatgac ggaaacggcg tgcctttggt gttgaaaaag ttgcgcgcgt ccatggcgga
     7081 attgcgcaga aactcgaaga cgctgccatg ccattgattc gtcccggatt tggtgacgac
     7141 atcgacgacg gcgccggagt tgcgtccctg gtcggcgctg taaaggtttg tctgaacctt
     7201 gaactccttg atcgcttcca cgccaggacg cagcacgatc gacagcgtca ggcggtcgtt
     7261 attgtcgatg ccgtcgtaca aaaaatcatt ggagccttca cgattgccgt tggagaagat
     7321 ctccgtaccg gggcggcggt cgtcgggccg cgtgccgctc ataatcgtgc cggcgacgct
     7381 gtatccggtg ccgttgacgc ccggcgaaag ggtcgccagt tgcacgaagt tgcggccgtt
     7441 caacggcagt tcttcggtca attgggtatc gatcacgctg ccgagcgtgg acgactggct
     7501 ctcaagcaag ggtgcggagg cagccacctc cacggtgtcg gtgacttgcc cgacctgcaa
     7561 ttgcagattg acttccgctc gctggttgac gcgcagctcg acgccgttcc gcaccgtctt
     7621 gcggaagccg ggggcttcaa cggagaggtt atagaagccg ggctgcaaca gcgtgaatat
     7681 gtacgctccg gatgaatcgg tactaccttc ccgcgacgtt ccggtttcgg aattcgtgac
     7741 ggtgactttg gcgctggcaa ccacgccgcc gctggcgtcg gttaccgtgc cgctgacttc
     7801 ccccgtggcg gtctgcgcga gcgcaggcag ggtggaaatc aggcaaagcg aaaccaacaa
     7861 gaacggcctc tggattttca tgacgccctc tcctttccac gagaccagat tgcattgagc
     7921 atacatcgtt caggtgctcg aaacaaccgg ccggtgggcg ggctgcctaa gtagagcaca
     7981 aaaggggtta cccggacgcg agcccgcgtt ataattttgg tcgtgcatcg ccattgggtt
     8041 ttagcctgcc ttctttgctt cgcctcgtcc gccggcctgg cccagcggag ccgtcagacc
     8101 ggtcccccgc cggctccgcc ggaacctgac caggagcagg aaaacggcga gccggagtac
     8161 acgttcaacc cgatccaggc ccaaaaggaa ctgaaaatcg gggattttta tttcaaaaag
     8221 aagagctacc gggcggccgc cggccgctac gagcgcgcga cgaagtggca gccggatctg
     8281 gcggaagctt actacaaact gggtgaatcg cgcgagaagc tcgatcagaa cgaacaggcc
     8341 ctcgctgcct atcaaaaata tctcgatctc tcccccccgc ccaaacaagc cgaagaagca
     8401 aggaagaaaa tcgcgcagct tcagaagcaa aggtcagcaa ctgcaagcaa atgagcagcc
     8461 cgccgccggc gcctgatttg ttcgaccggc gccccattct catcctgatc ctgctgacga
     8521 tcggcttttt ctggcggctc gtgctcagcc ggcagttcac gtttctggag agtccggatt
     8581 tggcctatca ggttctgccg tggtatcagg tcgaggcgcg cgcatttcac cacggcgttt
     8641 ttccaatgtg ggacccctat cagtggagcg gccaatctct gctcgggcag atgcagccgg
     8701 gagcggcgtt cccgctgaac tggctgctct ttttgatgcc cctcagggac ggacatctga
     8761 atttcgtctg ggtgcaccgc cactttgtcc tgatgcacat tctggctgcc cttttcatgt
     8821 acgccttctg ccggcgtctg ggttccagcc ggttcgcctc actgatggcg ggcgccgctt
     8881 ttgcgctggg cgggtatatg ggaacgacgc tgtggccgca gatgctcgaa ggcgccatgt
     8941 ggattcctct cgtatttttg tttttccacc gcatggtcga gcaagcgcag aagggctcct
     9001 cccggttgcc cgccctttcc agcgcggcgc tgtgcggcgg cacgatcggc gtttcccttc
     9061 tgagcgggca ccatcagacg ccgatgttca cgttgctggc tttgtgcggc gtattcctct
     9121 attttctttg cgcgcgtcca ggcctgcggc tccgcttgct gaccctgttc ggggctgtag
     9181 gcgtcttcgc gtttctagta agcgcactcc aactgctgcc ggctgcggag tacggcatgc
     9241 atgcctatcg ttgggtggga acggagaacc cagtccgcat gaatgagccc gtcccctatc
     9301 tcgcggcgga gaccggggga ttgtacccca tctcgctgct cggcgttgtc ttcccccgag
     9361 cgttcctcac caccgacccg tttctgggct tcgtttgtct cagctttgcg ctcttcgccg
     9421 tgttcacaaa ctggcggctg gccagagtgc gcatttactc ggtgctggcg ctcgccgcgg
     9481 tcgcctacgc ctcggggatc tactctgtgc tgcacgggct catctatgcg ctggttcctt
     9541 ttgccgataa agcccgcagc cccggccatg ccatatttgt tttccagttc gccgcgttta
     9601 cgctgacagc ccaggggatt gattgcttct tctcgccgca ggaaccggaa tgggagcgct
     9661 ggcgccaccg catcattaaa gctttggcgg cggccggctc gcttgcctgg atagccatgt
     9721 tttggcttta catgaacatg aagttcgaaa ccaagccggg cgatcacgtg gcgctcgcct
     9781 cgatggtcgc tttcgtgctg gccggcgtgc tttacggctt cggccggggc cgtctctccg
     9841 ccgccggcgc ccgagccgct ttgatcgccc tgctgctgtt tgagatgggt attgccagtt
     9901 actggattat tccccaccgc agcgatccct tgcgggcgca gtacctgcac cacctcaccg
     9961 atcatgccgg catcgtgcaa ttcctcaagg cgcagccagg gccctttcga ttcgacctgc
    10021 cgccgggcaa cgactttcct gccaacatgg gcgattggga gggtctcgaa ggcacgcgcg
    10081 gctatctggc atcggtttcg gccggattgt ttgacttcat gagctgggac tggacccaga
    10141 cgccgctttt gctaaataca gtttacgtgg tttcgaagac gcccacgcgc cccaaccagc
    10201 gcgaggtatt cgccgattcg gacgggtgga aagtctttcg caacgaagat gcgctcccgc
    10261 gggcctgggt agtgcagtcg gtgcgtatgg cgccgagcgc ccgcgaagcc gccgctattt
    10321 tcggcgcgcc tggtttcgac ccgcggcgcg aaaccgtgct gcttgcatcg agatcggaaa
    10381 agccgcctct cgagccttgc acgggcgaag ccgccgtaga ggtcacggga gttactttcc
    10441 atcatgtggc agcgcttgcg aggacgcctt gccgggccat ggtcgtcttc ggcgatcctt
    10501 attttcccgg ctggcgggct agggtagacg gctcgccggc ggccctttat gccccgttcg
    10561 gggcgctgcg cggagtgatt gttccgccgg ggacccacaa ggttgagctc gtctaccgcc
    10621 cgcgctccgt cttgattgga gcgctgcttt ccgccctggg atttatcggc tgcgcgtgct
    10681 tggcctggct ggcccgctcg cggtcgagcc aacggaccgc atcgcgatag ccggcttcga
    10741 tccattgatt gatttggcct ggattccacc gcagcatgtc ccggggccgt ccgagcggcc
    10801 ggctgggctc aatccgcagg acgcgcacgc ccggattggc gatctgactg acatcgcgct
    10861 gccgaatggc cgcctcccgc acagcgcagg ccgcacccaa agcggcgcgc agcactcgcg
    10921 acggaggggc agccagcaga tcgatggcaa cgatttcggc ggcgccggcg gcggccgccg
    10981 ctcctaaagg aagccggcag aaaacgccgc cgtcgcaata ccagcggccg tcgattttca
    11041 ccggagggta aaataaggga attgcgcagg aagccagcag gcacggcgcg gtgacctctt
    11101 catcggcctt cacgcatatt ttggcgcccg gcagttccgt cagcgtgacc agcagtttga
    11161 ctccgggcag cggttttgaa aaccgctcgc acagctcggt cacgcgcttt tcgagccgcc
    11221 gcggatcgag caagccccaa actctgggcg gccagttcca tcgaaaaacg ttccagcgcg
    11281 ggtcgcgcca ccacgcggag agctgcgcgg ccgtgcagcc gcgcgctatc agggcggctt
    11341 ccagggcccc cacggacgcg ccggcgacga tgtccggccg gaatcccgct tcctccagag
    11401 cttgccaggc tcccgcgtga tacgctccga acgttccgcc agccgataga acgagcgctc
    11461 gcaacggcct cccctgcctc tcatcttccc acggccccgc gcgcccggcg gccgctgcct
    11521 tatactgtcg gtatgcccga tgcatttccc gcgcacgagg agaccggctt gctggtcgaa
    11581 gtgcgcaacg gccctggagt tttgcaccag cttaccggcg tgattttccg ccacggcggc
    11641 gatatcacct cagtggcgat cctggacagc gacaagcccc tggcctcggt tttctttgaa
    11701 attgcgggtg tagatgccgt tgccgccatc gaagaggacc tgcaagccct cgatgtagtg
    11761 gcgagcgtag gccgcactac gacgctccag aaggtctacg ggaagcgcct catcatcatg
    11821 gggggaggcg cgcaggtcgg ccaggtcgct atcggcgcga tctccgaggc ggatcgccac
    11881 aatattcgcg gcgagcggat ttccgtggac acaattccgc tggtcggcga aaaggcgctg
    11941 gctgacgccg tgcgggcagt ggtgcggctg ccccgcgcga aggcgctggt gctggccggc
    12001 tcgctgatgg gcggggaaat cgaggccgcc gtgcgggagg tccgccagaa gggcctgctg
    12061 gtgatttctc tgaacatggc gggcagcgtt ccggaggccg cggatctggt tgtcagcgac
    12121 ccggtgcagg ccggcgtcat ggccgtgatg gccatcgccg atacggcggc gttcaacgtg
    12181 gcgcggctga aaacacgagt gttttgagcc ggccggaatg cgcttttcgg cccgcgcgtt
    12241 tttccacgga taaaccgggc cccagcggct gccagccggt gaagccgcga accggccgtt
    12301 ccctcaggat tccgtcctat taccaccagg gtcatatccc cgccggagta aaacaattca
    12361 ccagtctttg tttagggtgc tcgcccggtg ggaaatagcg ggttgttcga gcatactggg
    12421 gccatgagcg ccgctcagag tgccgccgtt ggcctgaatt tcgaggaaga cctgtacatt
    12481 ccaccggtgc cgcagacggt cgaggagacc ggactggcgc cgtcgcacat cgaacagctc
    12541 atcataaaga cgctctactt caatggcgag actgtggggc gcgatttggc caacgcgctc
    12601 gggctgaaat tcagcctcat cgacggcatc atggaattcc tcaagcgcac gcggctggtg
    12661 gaggtgaagc ggtcttccgg attcggcaat gtctcggcgg tgtttgcgct gtcggaagcc
    12721 gggcggatcc gcgggcgcga gtatctcgac atcaatcaat acgcgggccg cgcccccgtg
    12781 ccggtggagc agtacgtcga ggtggtgcgg aagcagcggg ccaaggatgg atggctgacc
    12841 aaggagacgc tgaccttggc gtttcagcac atggtggtca gtcccgagac gatcaccaga
    12901 atcgggcccg ccgtgaattc cggaaaatcg tttctgcttt acggccagcc gggcaacggc
    12961 aagacctata tggccgaggc gctgcaaaac gtggagcatt cctgcatcta cgtgccttac
    13021 gctctggaag tgcaaggtca gatcatccag atgttcgacg ccttgtatca ccgccgcgtc
    13081 gatgaagtgg atgcgtctca gtcttcgctc gccgtcgatc ccctgtacga cgggcgttgg
    13141 gcgcgctgcc gccgtccttt catcgtcacc ggcggtgaac tgactctgga aatgctcgat
    13201 ctgagctaca atccgtcggc gaaaatctac gatgcgccct tccagctcaa ggccaacaac
    13261 ggcatttacc tgattgacga cttcggccgc cagaaggtca gtccggccga actgctgaac
    13321 cgctggatta tcccgatgga ccgccgcgtg gactatctga ccttccacac aggcggcaaa
    13381 gttgccgtgc cattcgaggc ttttctgatt ttctccacca acctgcatcc cgacacgttg
    13441 ggcgatgaag ctttcctgcg gcgcatccag tacaaaatgt atctgaagag tcccactcag
    13501 gaggagttca tcgagatttt caagcgatac tgcgagtcgg aaaacctgta ttgtccctca
    13561 accgtcctgg atcgcttcat cgaaaagcac taccggcgca ccggcaagcg gtttcggcgc
    13621 tgccatccga gagatatcat cacccacgcc atcgatttga tccggttcga gaaagcgccc
    13681 tacgagctca ctgagcaaat gctggaccgg gccttcgaaa gctgcttcct cgaatccagc
    13741 gaaattgtag attaactgag cctctccaac gcggctgcga gacgcctcca gcggctgagc
    13801 ggatcagggg attgatttta cgtccgccca cggcgctggg tggtattctt gagaccactg
    13861 caatttcaca ggaggcgaaa tgctaggaga aatgctcggt gaatcgcgcg gcaagaggac
    13921 cggaagacga gtgctgccga tggacggacc cggcgtgaaa gtggaagttg cgtttgaaga
    13981 cagcggcaag ctgctgggga tcgacatgaa caacattgga acatattggt ccgcgacgcg
    14041 gcccgacggg actctctacg gggaaggaca gggcgttatc gtgacccgcg atggggatat
    14101 ggggacgtgg aagggcgcgg gcatcgggca gctcaagggc ggagccgtca gctatcgggg
    14161 agccattttc tactacagcg cttctcccaa actcgcgcgg ctgaacagca tcgccgccgt
    14221 attcgagttt gcggtggacc cggaaggaaa cacccatacc aagctttggg aatggaagta
    14281 agctgcgccg gttgctttca gcgggctccg cggcgcgcgc tgcccaaggt ccccccaatg
    14341 ggctcaggag cccgtcccgc gccgctccga atcttctggc atctcggaca aaagtgagtg
    14401 ccgcgttggg aaacgagaat gcgccggatc gcggcgccgc aaaccgggca gggaaggccc
    14461 gtgcggcggt agaccttgtg cagatgctgg aaagcgccgc gccggccgtc gcttcctacg
    14521 tagttggaaa tcgaagagcc acggtgcgca atggcctcgc ggagcacgcg gacgatggcg
    14581 cgataaagtt tcgccgcccg gccgggacgg atccggttgg ccaaagcccg agggtgaatg
    14641 cgtgcgcgga atagcgcctc atcgacatag atattaccca gaccgctcaa gaacgcctga
    14701 ttcagcaaca acggcttgac cattgcgcgc cgcgcgcgga agcgccgcac gaattcttcc
    14761 tctccgattt ccaggggttc actgcccagg cgtttaaatc gggcgggcaa acgctcggcg
    14821 aactccagcc gcccaaactg ccggacgtcg ttgtagagca gagcgccctt atcgagttga
    14881 aagagcacgc gggtgtaagg cccgggctct gccgccagcc ggagctgtcc tgtcatgccc
    14941 agatgcgcca gcagatgtcc ccggcgtccg ccgcgctcca aatccagcaa cacgaatttt
    15001 ccacggcgcc ggacctgggc gatggtgaag ccttcggccc gctggatgtc gccaatgatc
    15061 actcgaggct gaaatacttc gatccgctca atccgccggt ttggaaggcg ggaggccaga
    15121 ccgcgcacga tcgtttccac ttccggcagc tcgggcatac tacgcgcgcc attcgggagc
    15181 aagtccggcc acaaattcgt cgaagttccg cggcgcgtgg cccagaattg cttgctgctg
    15241 atccagatcc tctttcgtag cagccagccc gtgcgcctga aaatactcgt acatgatgcg
    15301 gagatcgcgc accagccagt ccggcagcat gcggcgcatc tgctccgccc acgaatccag
    15361 gttgtcgccg gcatagcgga cctcccggcc gagccgccgg ctgtaaatcc gggccgtact
    15421 ttcgccggtg agaaggtcgg gtcctaccaa ggggtatttt ttgccgttgt gtccggcctc
    15481 tgtcagcgcg cgggcggctg cgtcggcgat atcgcggacg tccacccggt tcagcccgac
    15541 atttccgatc ggctgcgggt agacgcccca cctgacgatg gcctcgcgca tcaaaaggtc
    15601 attctgaaag aaattattcg gcgcgagtat cgtgtgctcc agacctgatt cccgcaccga
    15661 cttttcaatg ggcagcttgc tcttgaagtg cgggatgtgc ggggcgcgct ccagattgtg
    15721 caccgacatg tatacgaagc ggcgaacacc cgccgagcgg gccgccgcca ccgccgccaa
    15781 tcccgcttgc gtctcggtag gactgagagg tgtggcgagg aagaccgcat ctattccccg
    15841 gaaggccccc ggcaggctgg ccggcgcgtc gagatcgccc gtcaccgctt ccgcgccccg
    15901 gggtacggct ttcgcttttt cagccgagcg agtcatgatc cggacgctct caccgcggcc
    15961 gaccaggttc cgcgctgtct ggctgccgac cgtccctgtg cccccaatga ccagaatttt
    16021 catggagctt cctcctcctc gttgctcgcg accgccggcg agaatgcgcg gcccggggac
    16081 cgcgcattct cactcaggac gctcgccgag ctcgtaagtt gtgatttcta tctcatggcc
    16141 gtccgggtcc cggaaataga ttgagtgaca aatatcgtgg tcttcaaagt ggaatgcgat
    16201 atttcgcttt tgagatcaac ctgcgcctcc tcaaaattgg cgcggtttgt ccggaaggca
    16261 agatgccgga tcaccgtagg gcgtttggcg cgcggcaaat ggccgggctc ggtgcttgcc
    16321 gccggaaaca gcgcgatggc tgtgctgccg gcgcacatca cggcaggaaa gctgccccat
    16381 gcttcctggt aacgccgctc caagccgagc acgtcccggt accacgcgac agacctctcg
    16441 acgtcggcaa ccgtcagcgc aatatgatcg atttgctcga ccttaaacat tggcgggctc
    16501 tagaccagat cggaaggagc gcaagcgcat gaaccgccgg ccccgattgt ggccgcacag
    16561 agccggtatt cggcgcgcag cctcggctga tcaagcggct ggattctgga cctgcgcgac
    16621 catcacccgc ttcttgaact gttcgagcat gctctcgtcc agccgcacct cagtgggcct
    16681 cttcaccagc gtggtcatct gatcgatttt gtcttgcagc ttcagcagcc cgtaaaagag
    16741 attctcgggg cgcggcgggc aaccggtgat gtagacgtcc acaggaacga tccggtccac
    16801 cccttgcagg actgcatagg tattaaaagg accgccaacg ctggagcagg cgcccatcga
    16861 aatacaccac ttcgggtcgg gcatctggtc ccagatgcgt ttcagaaccg gcgccatttt
    16921 cagggtcaca gttccggcga cgatcatcag atccgactgg cgcgggctgg ggcggaacac
    16981 ttccgagccg aagcgggcga tgtcgaagcg cgaggtcgaa gaagcgatca tctcgatggc
    17041 gcagcaggcc aagccgaagg tgaggggcca aatcgccgat tttcgcgccc agttgaagac
    17101 gtaatccacc gaggtggtca aaaagttctt ctcgaactga ttttggatga aggccatacc
    17161 gaattcattc taatacaacc agcggagtat gcggagccgc aggcggctgc gagccgggcc
    17221 gtaaaaccgg cttgcgcgcc tcgatcagca aagcgcccgc gcggcggaag gcgagcttgt
    17281 cttccggggt cgggtagaat tcgctgggcg caaagccagg ctcgacgggg ctctcgtcgg
    17341 tgatttgctc gtggcgcacg gcttcgaaac cgatggctgc gaaaagctcg gcccattccg
    17401 cgccggcctt caggtgaacg ggaacgcgaa agaggccctg ccagcgttta tgcatggggt
    17461 tttcccgaaa gtagctcaac agcagaacca ggcggccgcc cggggagagg atgcgccgga
    17521 tttcggaagc agccgcggcc gggtcgggcc aataaaaagc ggagtccacg ctcaaggcca
    17581 gcgagaaaaa atcttcttgc cagggaattt catcggcggc gccggagaca aacatgcaat
    17641 tctcgagcgc cacggaaagg cggcgggcga gccggatcat ctcgtcggag atgtccatgc
    17701 ccaccgccag cccctctgat gccatctgag ccagacgccg cgtcgcccag ccggagccgc
    17761 agcctatatc gagcgagcgc tccgcccgag ccacctgcat ccggtcgaat actttttcca
    17821 tgatcgccgg atgccggtat tcgagacgcg gattcgccag ggcgcggcgg tcgaactcgg
    17881 cgcgcagcag cgcctcttcc gcttcagtca ttcccggcta tggtcaccaa ttgcgggcgc
    17941 gccaggccgg cggcgaccgc ctcggcctcc tcggcggtga agccctccac ggtcgatgaa
    18001 atattcatcg agcagaagcg cgggccgcac atcgagcaga agtgagcttc tttgaagcct
    18061 tcctgcggca aagtctcgtc gtgcagcgcg cgggccgttt cgggatcgag ggaaagctcg
    18121 aactggcgat tccagtcgaa gttgtagcgg gcatgggaga gcgcatcgtc ccggtcgcgg
    18181 gctccaggcc gatgccgggc gatgtcggcc gcgtgggcgg cgatcttata ggcgatcaag
    18241 ccggccttca catcctcctt gttcgggagc ccgagatgct ccttcggcgt gacatagcag
    18301 agcatcgaag ccccgtgcca gccgatcatc gccgctccaa tggcggaggt gatgtgatcg
    18361 tatcccgggg cgaagtcggt gacgagcgga ccgagcgtgt agaacggagc ctcgtaacac
    18421 cactcccttt ccttttcgac ctgcatctgg atttgatcca tagggatgtg tcccggcccc
    18481 tcgatcatca cctggacgtc gtactcccat gccttcttgg tcaactctcc gagcgtctta
    18541 agctctgcga actgggcttc atcgctggcg tcggcgaggc agcccggccg caagccgtcc
    18601 ccgagcgaga agcttacatc gtatttctgg aaaatcttgc agatctcgtc gaagtgctcg
    18661 tagagcatgt tctgcttgtg attcttgacc atccattcgg ccagaatcga gccgccccgg
    18721 ctgacgatgc ccgtaatgcg cttcacggcc agcgggacgt attggatcag cacgccggcg
    18781 tgtatggtca tgtaatccac gccctgttcg gcttgctctt cgatcacctc caacatgagc
    18841 tgcggcgtca gatcctgcac gcggcggacg cgcgtgaggg cctcgtagat ggggacggtc
    18901 ccgatcggca ccggcgaatg agcgatgata tgctttcgta tattggggat gtcgccgccg
    18961 gtggagagat ccatcaccgt gtcggcgccg tattttatgg cgtagcgcag cttctccagt
    19021 tccccgtcca catccgaggt gaccgccgag ttgccgatat tggcgttgac cttgcaagct
    19081 gaggcgacgc cgatgcacat gggctccaga tt
//
LOCUS       DASREK010000085         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2192663, whole genome shotgun sequence.
ACCESSION   DASREK010000085 DASREK010000000
VERSION     DASREK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192663"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFA54_04530"
     CDS             <1..542
                     /locus_tag="VFA54_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZS50103.1"
                     /translation="LAQRLGCRELNLLTNALAENGASGHPAAPHPATMWITAYTTLSQ
                     VAELAERHDVVYNLEPLNTKLDHPGYPLPRVEDVVRLVEKVGSPRIKLLFDVYHAQVQ
                     EGNVIQLIRDFHSYIGYVHVADVPGRHEPGTGEINYPHVAKALMQAGYDGIIGLEAFP
                     AGDDAEAMRRFREIFAAQE"
     gene            650..1582
                     /locus_tag="VFA54_04535"
     CDS             650..1582
                     /locus_tag="VFA54_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZS50104.1"
                     /translation="MAGAAVLGACRSSHKKVIAVIPKGTIHIFWQSIQAGALAAGHDW
                     NVEVVWNGPAEETDYSRQIQIMDSMITRRVDAIAVSASDRIALNGALDRAAAAGIPVA
                     VFDSGVDSTNYVTFLATNNYQAGEMAARELGQLLHGKGKVAMLMHLPGSGSTMEREKA
                     FDDVMTKEFPGIQVVARQFGMSDRAKAMAAAENILTAHPGLDGLFGSSEANSVGAAQA
                     FKGRGLAGKIRLVGFDYSDGLLADLRSGTIDALVVQDPFRIGYEAVKTLVDKLNGRTP
                     PKRVDLSARVITKADLEKPEVKELLFRDLGKYLH"
BASE COUNT          406 a          581 c          620 g          396 t
ORIGIN      
        1 cgctcgcgca acggctcggc tgccgcgagc tgaatctgct gaccaatgcg ctggcggaaa
       61 atggagccag cgggcatcct gccgcgccgc atccggcgac catgtggatc acggcttaca
      121 cgaccctgtc gcaagtcgca gaattggccg agcggcacga cgtggtctac aacctggagc
      181 cgctgaatac caagctcgat catcccggct atccgctgcc gcgcgtcgaa gacgtcgtgc
      241 ggctggtgga gaaagtcgga agcccgcgga tcaagctgct cttcgacgtc taccatgctc
      301 aggtgcagga gggaaatgtc attcaactga tccgcgattt tcactcctac attggctacg
      361 tgcatgtggc cgatgtcccg ggacggcatg aaccgggaac aggcgagatc aactatcctc
      421 acgtggcgaa agcgctcatg caagccggct acgatggcat catcgggctg gaggcgtttc
      481 ccgccggcga tgatgcagag gcgatgcgtc gttttcgcga gatttttgca gcacaggaat
      541 agaagcggtg tccccgagat cagcggcgaa cgaaacggcg ggccgctcgt ccttggcgcc
      601 gcggcggcgg caaactttcc gttttccttg gcgggttttc gttctcctca tggcgggagc
      661 cgcagtcctc ggcgcctgcc gttcctctca caagaaagtc atagcggtta ttccaaaggg
      721 cacgatccac atcttttggc aatccattca agccggggcg ctggcagcgg ggcatgactg
      781 gaatgtggag gtcgtctgga acggtccggc ggaagaaact gattacagcc ggcagatcca
      841 aatcatggat tcgatgatca ccaggcgtgt ggatgcgatc gccgtttccg cgtccgaccg
      901 tattgccctg aacggcgcgc tcgaccgggc cgccgcggcc ggaatccccg ttgccgtctt
      961 cgattccggc gtcgattcca cgaactatgt gacctttcta gcgaccaaca actatcaggc
     1021 gggcgagatg gctgcgcgtg agctcggcca gctcctccac ggcaagggga aagtagccat
     1081 gctgatgcac ctgcccggca gcggctccac gatggaaaga gaaaaagcct tcgacgacgt
     1141 catgaccaag gaatttcccg gcatacaggt tgtggccagg cagttcggca tgtccgatcg
     1201 cgccaaggcc atggcggcgg cggaaaatat cctaaccgcg catcctggcc tcgacggatt
     1261 gttcggttcg agtgaggcca attccgttgg ggcggcgcaa gcgttcaagg gccggggtct
     1321 cgccggcaag atccgcctgg tgggcttcga ttacagcgac gggctgctgg cggatctgag
     1381 atccggcacg atcgacgccc tggtggtgca ggatccgttc cgcatcggtt atgaagcagt
     1441 gaagacgctg gtggataaat tgaacgggcg gacgccgccg aagcgcgtgg acctcagcgc
     1501 ccgtgtgatc accaaggccg acctcgaaaa acccgaggtc aaggaactgc tgttccgcga
     1561 tttaggaaaa tatctccatt aggcggccga agacctgcct tgaaccctct cgcgtgaggg
     1621 gacttatttc ttcaagccct cttatagccg cggccctgcg cgtaacggag tattgaaccc
     1681 tctcgcgtga gggacttatt tcttcagggg gcggtggtac tagaaactag cggtacgtat
     1741 gactaatttc cactcggcgg ttggcgctct cccacgggct tttcggaaga aaatttgtgg
     1801 tcaaaattcc aaccatcccc gataatagtc ctgatgcctc gaaggggagg gcggcagtgg
     1861 acagggattc tccgacgagc aagaagcatc ttgcgagagc agggattccg gggcctcatt
     1921 tggaaaactc tcggtgagac tgtcttccgc cgcatgcgct acttcgagcg cgccttggcg
     1981 gagccgattc cccaaattgt ccc
//
LOCUS       DASREK010000086        13698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1037027, whole genome shotgun sequence.
ACCESSION   DASREK010000086 DASREK010000000
VERSION     DASREK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037027"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFA54_04540"
     CDS             complement(<1..274)
                     /locus_tag="VFA54_04540"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HZS50105.1"
                     /translation="MEAWRQARPRQAAKLIDLARSGLEGWDIFGVPVALDPARIPWRL
                     GDTRFVWEINRHQFFFTLARAFSLTGDRRYALRVFSLVEDWLNKNPC"
     gene            complement(519..1496)
                     /locus_tag="VFA54_04545"
     CDS             complement(519..1496)
                     /locus_tag="VFA54_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50106.1"
                     /translation="MRKRFESGRPRYGRLLLAILLLPGLALTEDRPVIKAVVSSADFQ
                     PGAAFEGLATIFGTGFSSQEYHAASLPLPTKLGDIQVRYCVPLSAVWDDCIPMELLYV
                     GPNQINFRISYGSRDESQPVFNGALVVRRNNTLSDETRRQVFDPFAPRIFVMGFDCNF
                     DPSYHDASPCGLTPQRASAQQVVRGALTDVAGVLITSNNPAQTGRAYTLWLTGLGHLA
                     EGQPADVQVRIGGLSSGGIPANLAASAQLLYAGASPQFPGLYQINFELPPQLAQNLAS
                     AGIAGPSSQNSCGDYHLELAVSVSEGAAVASNTVEMPLVAFNGDGPCSR"
     gene            complement(1518..2996)
                     /locus_tag="VFA54_04550"
     CDS             complement(1518..2996)
                     /locus_tag="VFA54_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HZS50107.1"
                     /translation="MRLLANRHLGPAKGAPPADQTRPEAAIHPKSAYPAHGPLALTAA
                     AAKRVAGNALVLAALKGISPLISMLLLMVICRRLGAEGMGRYALAYAYLNVFILLGSL
                     GLPTLLTREGARHSHSLSALLASAMALTGALSLALTALMTALAVLSDFDVQTVRAMIV
                     LSLAIAPSICLSCYEAAFLSREETLPIALAALAENVFKAGLGIAAVLTGCGIDAVLAA
                     AVAGRVAACGLSFVLLRRRGILVTWTANVGSARKLAAQTPVFALSSVCATLYWRADIF
                     LLSRLRPLSEVGCYAAAYRILDMAILPPQSLCQALYPRIARDSQTAATGRNPALLGLA
                     LVTIPLAAAVTCLAPALPRLLYGRAFWTAAPTLMILIWTTVPYAWNRYHANVLLAHDS
                     QRADLLINVTLLALNIALNLILIPRYGANGAAAVTLASAILYGAAQARYLKSRTRAGN
                     RPRKSERNPPFATAFFGPRMGEGIIRHRRSPKQFASGPEPAG"
     gene            complement(2993..4393)
                     /locus_tag="VFA54_04555"
     CDS             complement(2993..4393)
                     /locus_tag="VFA54_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS50108.1"
                     /translation="MTRVETFGRLSLWLSASGGVAAGLAIVWLAEMPVRWVTAVLLGA
                     GIVAAALMTGQPRRAALFLTAFSLQIGLVLYLTKPEPASDLGASWPNSLALPLGTLAA
                     LGALLCGVRRRFTWGGTAGLCAGIVAFTTAASLPGSPRPFIGVCHVVLVFAYYAIFLA
                     AANCVRELRDLRLINHALMISLALQSLVYFAQSLVGATFTPAGEWIEQTGQPLGRYGG
                     WAGTQPAAFASFLLPLLLLAVARFLTARDLARLHPAMLLTVMGLSTLCLTFTRAAWIG
                     LGLAVIYLVGAAARRAMLVRRNALLLAVCLVAVAGALGPGISVRVRQDHASALAERWS
                     LIRMGLQVARAKPLAGVGAGAYPYVFENYLTPDLAKGWLYVVHNVYVLRAAETGLPGL
                     AALVFLLGAFFRLASPERMQEAEARVMALGWRAGLIALSWEMLWDVSLGPAANSLLWF
                     LSGLMISAARFSPQRP"
     gene            complement(4380..6212)
                     /locus_tag="VFA54_04560"
     CDS             complement(4380..6212)
                     /locus_tag="VFA54_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZS50109.1"
                     /translation="MEGLLEANILGIRIRRRTQREALDCLRKMLAGRGPHSVYFVHAA
                     TANLAFENAEYRAILNRGDLVLNDGVGVRWAGRAAGAPFPDNLVGTDLLPQLFQERSR
                     ENQLRLFLLGGRPGVAQRAAARLEREYPAISVVGFHHGYFSPGAERDIVNQVRRFKPD
                     LLLVGMGNPRQEEFIDRRLHELGCKAAIGVGGLFDHLAGELRRAPVWVRRLGLEWLQL
                     LTQQPHKRRRYLCGIPRFLWRVFWPKLVGQSRRLAKAVLGAGAGVAIGLLAGEGVVRL
                     ARPQILARYPEGLYVPSASRQYKLRPHFSGTFRYPEFRTQVRINGQGLREDQEYQQPT
                     PQICRILAVGDSFTMGYSVDQQRTWVRTVERLLNTELPAPGQAGRDQGRVHFPPRRRY
                     EVINAGVPGYSTWQELAYLEEEGLSLKPDVVLLGFFIGNDIADNASATLPVELRDGAL
                     ISAGARNGCLPFVLRLALARHSHLYYAAHSIGARDPRRYAIYTGGAGDGWRQTEQLFL
                     RLSEIAKRHTLRAIVLLIPERMQVESALARAAAAEIGRPIDAAAPSRRMREISRRAQL
                     ETIDLLDRIAGMTGLYFAQDGHWNAQGNLAAAAAIFEYLRHDPS"
     gene            complement(6223..7647)
                     /locus_tag="VFA54_04565"
     CDS             complement(6223..7647)
                     /locus_tag="VFA54_04565"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZS50110.1"
                     /translation="MHHFRRSLLAEGLKIFDLAALSACLGFAVLLPAAPARPTGLAGV
                     LSLRISLRSVLLAFVFLVLCRRIFECCGLYQSQRLGSRKKFYKAILRAVTLAALLLGG
                     LLAAFGESAFHGASVLEFWAAALTLVAFSRVFLYTSLAAVRRRGRNLRSILIIGAGRR
                     GRSFAESIQRRPELGYRVVGFLDDSGGGPGPAPLLGGIGDLPRILREEQIDEVALALP
                     LKSFYSQASRLVSFCEEQGIVIRLPGGLFEAPLAQVESERLDELGVLTLSTVRGSAWS
                     FLLKRIADVVLAAAGLLATLPVWPIIAVLIRLDSAGPALFRQVRVGYNGRRFLMWKFR
                     TMTVDAEARQAELEPLNEVLGAAFKIRNDPRITRVGRWLRRTSLDELPQLLNVLKGEM
                     ALVGPRPLPVRDVERLSGGWQRRRFSVRPGITCLWQAGGRHRLGFEDWMRLDLHYIDN
                     WSLLLDLKILLRTLPALWTGAGAS"
     gene            complement(7703..8476)
                     /locus_tag="VFA54_04570"
     CDS             complement(7703..8476)
                     /locus_tag="VFA54_04570"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HZS50111.1"
                     /translation="MSRYLEILRKAKLEGELFSTPREPGARRSSAPAVSTPPSSPSPW
                     SRQGTQHRDQWQQLVYRLFLPPAARLPRSIAMASATSGEGTSYVAFHLAVQAARATGN
                     PTLLLEANLYRPSQAGRFGLSGSPGLREILHDEQFPLEACLHQTAFDRLWLLPAGSAG
                     NGLAGLPDWTNFRGVFFRLRQRFPAIVGDLPPVNTSDALILGPFFDAAVLVVEADLAS
                     REVIQNAATRLRRANSSVAGAVLNKRKFFVPEPLYRRLG"
     gene            complement(8492..9784)
                     /locus_tag="VFA54_04575"
     CDS             complement(8492..9784)
                     /locus_tag="VFA54_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS50112.1"
                     /translation="MFVEHFGLREQPFGSTPDPKFLYFTAQHAEALAALHYGLSERRG
                     LLVLTAEPGMGKTTLLHYLMDRWRDRAQMAFLFSPPENRDQMIAAVLEDLGLACTGDF
                     SEKRQMLQALALECHRQGRRLLLVFDEAQAIPAPVLEDIRLLSNFETPQEKLMEIVLA
                     GQPALSERLESPECEQLRQRICLHAGIGRLNSEDVARYISHRLRVAGVKRRIFSRRAL
                     ELAAEASGGVPRKINTLCFEALSAAFLKGKKRVGREEIHSAATAAAVRFPATAPRKSL
                     AAGLGWAAGFASLALLAGTMGFESTLPAVKQALHFRTPQPGEILVAPAAASPAALPET
                     APAASRSAEGSDRPPESPTEEQPATELPIAERTPETFPAARVSVAPGETLRRIALREY
                     GQWNEEIWQWIRRANPQLTDPDNLKAREVLLLPAYPKE"
     gene            complement(9794..11269)
                     /locus_tag="VFA54_04580"
     CDS             complement(9794..11269)
                     /locus_tag="VFA54_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50113.1"
                     /translation="MRRENSSVDLVPFTGELRRGEAVTGPRDLAAAFFRRKAIMGVLF
                     GSVLAGAAVGAIYLRPRLFPPRYASELRFILRKDRFDAVVTPFDRAVPTLNTTVAPQE
                     IQSEIEILRSSDVLDRLAREARVASPERLDQGLTVEPVTAGRNFTNLIAVRYSSPDPA
                     EVARVLEALPRIYAEKYVAVNRRPAAVEYYRSQADDFEQQLRQAEADLAEFEKGQPEL
                     VAVDVAQRARQKLSDLEKQKSDSESAIREARSRVAELTRELAVIPVTIRSARQIGENP
                     YLERLQLELLEAQNRRSEAKFYREIDRLETRIGELRQQIQEAQSHRRSFEENLPNPLR
                     QTVEGELVRAKAALAGLQSRLASISEQEHKAREELAASRLIAAENASEKAELSRNVKI
                     AEEGYMLYRKKYTEAEEAEALDQKGVLNVSVAEPARPPFRVASRGVWFYFAASVLLAA
                     AVAAAGGFAAELADHSIHTPAQLEAQTSLSVLACIPESKRR"
     gene            complement(11496..12215)
                     /locus_tag="VFA54_04585"
     CDS             complement(11496..12215)
                     /locus_tag="VFA54_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS50114.1"
                     /translation="MPIHVFAASSLELTGEAITQCLREQPDVLARVATGSPQEVLNQC
                     AGFHPCVLIIGDSFLDSIDPRRFRELIDYGRAISVLVIGSGGAPEEAMSWLQLGCLGY
                     LDRSETLATITRAVYAVAAGQFWAPRSQVADLLRHLLDGGGASSQLTGREEQILELIA
                     QGRSNREIANSLFISNETVRWHVRRLLAKIGAEDRAAAARFAQRYWAGSRFPILPRRS
                     ARAAQATAPSSGGRPVSASAT"
     gene            12327..>13698
                     /locus_tag="VFA54_04590"
     CDS             12327..>13698
                     /locus_tag="VFA54_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HZS50115.1"
                     /translation="MELFFGKRDKLPHASLVCLLRGQVPELTAGSSAYIPPKNKMRWQ
                     KIIAGWQLGAALACAAELSPVALRVAGQLTNRANGFNLVEGRELAGPQGLAIDASATP
                     PHIYVADTLNNRVLGWRDAGNFPTGAVADLVIGQPDLLGTGPNSPDVSVGFWSPAGVA
                     VDAAGNLFVADTNNHRVLRYPRPFDQKNQAPDLVVGQMNFSSHSRNMGLADPNSASLA
                     YPVAVALNAQGDLAVSDSVNHRVLIFRAGSLAANGPAADVVLGQANFSTSAGGAGPAG
                     ISQPGGVAFDGTGRLYVADVTGNRVLVFSAGAATGASAVRIIGGNPDGALRGPHTLYR
                     PFGVAAAGDMLLVADTGNHRVLGFHGLSAAAGAVAAADFVFGQPDFTSALPNAVSEAR
                     PLASARALCSPAQAALGPDRTMFVADAGNNRVLAVPPAGAAYVRAARVLGQAGFTQNA
                     PNRVDGR"
BASE COUNT         2798 a         4362 c         4161 g         2377 t
ORIGIN      
        1 ggcaaggatt cttgttcagc cagtcttcga ccaagctaaa gactctgagc gcatagcgcc
       61 ggtcgccggt gagcgaaaag gcgcgcgcca gcgtgaagaa gaattggtgc cggttgattt
      121 cccaaacgaa acgcgtgtct ccgagccgcc aggggattct cgccggatcg agagcgaccg
      181 ggacgccgaa aatatcccag ccctccaggc cagagcgagc gaggtcaatg agcttcgccg
      241 cctgccgcgg ccgtgcctgc cgccaggcct ccaatattcg ccttccgcgt tcgggaccaa
      301 atagaaaaca tcggtcgagc cgcccgcaca gggccaatcg gtcgggcgga gtccattttg
      361 caggaacaaa agaatggcga ctgccgccat gcatcgccag cctgtgaatc atttcgcgcg
      421 gactcatagc gaccagccgc tgcgcgtaac ggagaggctt cgctaccgcc ggctgacaat
      481 gccatttcaa aagcggtgtg gcaatttctg atctggtctt accgggaaca aggtccatcg
      541 ccattgaacg ccacgagcgg catctcgact gtattactcg ccacagccgc tccctcggag
      601 acgcttacag cgagctccag gtggtaatcg ccgcaagaat tctgggagga cgggccggcg
      661 atccctgcgc tcgccagatt ctgcgcgagt tgcggcggaa gttcgaagtt gatttgatac
      721 aaacccggaa attgcggaga agcgccggca tatagcaact gcgcgctggc cgccaaattg
      781 gcgggaattc cgccggagga caggccgcca attcttacct gcacatcggc cggctggccc
      841 tcagccaaat ggccgaggcc tgtgagccaa agggtgtaag ctctgccggt ctgcgccggg
      901 ttattcgaag tgatcagaac gcccgcaaca tcggtcaggg cgcctcgaac cacctgctgg
      961 gcgcttgctc gctgcggcgt gaggccgcag ggcgatgcgt cgtgatagct gggatcaaag
     1021 ttgcagtcaa agcccatcac gaaaatgcgg ggcgcaaacg ggtcgaacac ttgccgcctg
     1081 gtttcatcgc tgagagtgtt gttgcgccga actaccagcg cgccgttaaa aacgggttga
     1141 gactcatcgc ggctgccata ggagatccga aagttaattt ggttgggacc gacgtacagc
     1201 agttccatcg ggatgcaatc gtcccaaacc gcgcttagcg gaacgcagta ccgcacctgg
     1261 atatcgccca gcttcgtggg cagcggcagt gaagcggcgt gatactcctg gctggagaat
     1321 cctgtgccga agatcgtagc aagaccttcg aacgccgccc caggctggaa atcggctgaa
     1381 ctgacgaccg ctttgataac cggccgatct tcggttagtg ccagcccggg cagaagcaag
     1441 atagccagaa gcaggcgtcc gtatcgtggt ctgccggatt cgaaacgttt ccgcacgcgc
     1501 gtggctccgg taactggcta gccagccggc tcgggtcccg aagcgaactg cttcggggat
     1561 ctcctgtgac gaatgatccc ttcacccatg cggggcccga aaaaggccgt ggcaaagggc
     1621 gggttgcgct ctgattttct cggccgattt ccggctcgcg tcctgctctt cagatatctg
     1681 gcctgcgccg cgccatagag aatcgcgctg gccagcgtga ccgccgctgc tccgttcgcc
     1741 ccatatcgcg gaatcaagat tagattgagc gcgatgttca aggccaaaag cgtcacattg
     1801 atcaacaaat ccgcgcgctg cgaatcatgc gctaaaagca cattggcgtg ataccggttc
     1861 cacgcgtaag gcacggtggt ccagatcagg atcatcagcg tgggcgccgc agtccagaag
     1921 gcgcggccat acaacaggcg cggcaaagcg ggggcaaggc aggttacggc tgcggcgagg
     1981 ggaatggtta ccagcgccag gccaagcagc gcggggttcc tgcccgttgc ggcggtttgc
     2041 gaatcgcggg caatgcgggg ataaagcgcc tgacacaggc tctgcggcgg caaaatcgcc
     2101 atatcgagaa tccggtatgc tgccgcatag catccgacct cgctcaaggg ccgcaagcgc
     2161 gagagcagga agatatcggc gcgccagtag agggtggcgc aaaccgagct cagcgcgaag
     2221 actggcgtct gcgcggcaag cttgcgcgcc gatcccacgt ttgccgtcca tgtcaccagg
     2281 attccccgcc tgcgaagcaa aacaaatgac aggccgcatg cggccacccg cccggctacg
     2341 gcggcggcca gcacggcatc gattccgcag ccggtgagca cagccgcgat tcccaatccg
     2401 gccttgaaga cgttttcggc cagcgctgcc aaggcgatcg gcaaggtctc ttcccggctc
     2461 aggaacgcgg cctcataaca actcaggcag atggaaggag caatggccag gctgaggacg
     2521 atcatcgccc gcacggtctg aacgtcaaag tctgacagga ccgccaaagc cgtcatgagg
     2581 gcggtgaggg ccagagaaag cgcgccggtg agcgccatcg cgctggcaag caaagcgctg
     2641 aggctgtgcg agtggcgcgc cccctcccgc gtcaacaatg tgggcagtcc gagagatccc
     2701 agcagaatga aaacgttcaa gtacgcgtag gccagcgcat agcgtcccat cccttctgcg
     2761 cccaagcggc ggcaaatcac catcaggagc agcatggata tcaggggtga aatccccttc
     2821 agggccgcga gaaccagcgc gtttcccgct acacgcttcg ctgctgccgc ggtaagggcg
     2881 agcggcccat gcgccggata cgcggacttc ggatgaatcg cagcttcggg ccttgtttgg
     2941 tcggccggcg gcgcgccttt cgccggcccc agatggcgat tcgcaagcaa tctcatggtc
     3001 gttgtgggga aaagcgggcc gccgatatca tcaatccgct caagaaccaa agcagagagt
     3061 tggcggcagg cccgagagat acgtcccaca gcatctccca agaaagcgcg atcaatccag
     3121 ctcgccagcc caaggccata acgcgcgctt ccgcttcctg cattcgttcc ggcgaagcga
     3181 ggcggaaaaa agcccccaga agaaaaacca gagcggccaa accgggcaaa cctgtctccg
     3241 ccgcgcgcag cacgtaaacg ttatggacca catagagcca gcctttggcc aagtccggcg
     3301 tcaggtagtt ttcaaatacg tagggatagg ctcccgcccc gacgccagcc agcggcttgg
     3361 cccgagcaac ctgcaaaccc attcgaatca gagaccagcg ttctgccaga gccgaagcat
     3421 gatcctgccg tacgcggacc gaaattccag ggccgagcgc tcccgccaca gccacaaggc
     3481 agactgcgag caagagagca ttccggcgca ccagcatggc gcgccgggcc gccgccccca
     3541 ccaggtaaat gacggccagc cccaggccga tccacgccgc tcgggtgaac gtaagacaca
     3601 atgtgctcaa tcccatgact gtgagcagca tcgccgggtg caggcgcgca agatcccgcg
     3661 cggtcaggaa acgggcgaca gcaagaagca gcagcggcag caggaaactc gcgaaggccg
     3721 ccggttgtgt tcccgcccag cctccgtatc tccccagggg ttggcccgtt tgctcgatcc
     3781 actcgccagc cggcgtgaat gtcgcgccga cgagactctg agcaaagtag accaagcttt
     3841 gcagcgccag cgagatcatc agggcatgat tgatcagacg gaggtcccgc aactcccgaa
     3901 cgcaattagc agccgccaag aagatcgcgt agtatgcgaa tacaagcaca acgtggcaga
     3961 cgccgatgaa agggcgcgga gaacccggca gggatgcggc cgtggtgaac gcgacgattc
     4021 cggcgcaaag gcctgccgtg ccgccccagg tgaaccgccg ccgcactccg cagagcaggg
     4081 cgccgagcgc cgcaagcgtt ccaaggggca gcgccaggga attcggccag gatgcgccga
     4141 gatcggaggc cggctcgggc ttggtcaggt aaagcaccag gccgatctgc aagctgaacg
     4201 ccgtcaggaa caaggcggcg cggcgcggct ggccggtcat cagcgctgcg gcgacgatgc
     4261 ctgcacccag caacacagcc gtgacccagc gaacgggcat ttccgcgagc cacacaatag
     4321 ctaggcccgc agcgactccg cctgatgccg aaagccataa ggacagccgg ccgaaggtct
     4381 caactcgggt catggcgcag gtactcaaaa atggctgccg ccgccgctaa attgccctga
     4441 gcgttccaat ggccgtcctg ggcaaaatag agcccggtca ttccagctat gcggtccagc
     4501 agatcgatgg tttccagttg cgcccgccgc gaaatctcgc gcatccggcg gctgggagcc
     4561 gccgcatcta tcggcctccc gatttccgct gcggccgccc gagccaaggc tgattcgacc
     4621 tgcattcttt ccgggattag caagacaatc gcccgcaggg tgtggcgctt tgcgatctca
     4681 gagagacgca gaaagagctg ctcggtctgg cgccagccat cgcccgcccc tcccgtgtag
     4741 atcgcgtagc ggcgcggatc ccgcgcccca atgctatgag cggcataata gagatgtgag
     4801 tgccgggcaa gggccagtcg caagacaaaa ggaagacagc cgttgcgagc cccggccgag
     4861 atcagagccc catcgcggag ctcgaccggc agggtggccg atgcattatc ggcgatatca
     4921 tttccgataa aaaacccgag taagaccaca tcgggtttca gcgacagccc ttcttcttcg
     4981 agatacgcca attcctgcca cgtggagtaa cccggcacgc cggcattgat cacttcatag
     5041 cgccttcgag gagggaaatg cacccggcct tgatcgcggc ccgcctgccc cggtgcgggc
     5101 aattccgtat tgagcagcct ttcgacagtc cgcacccacg tgcgttgctg atccacgctg
     5161 tagcccatgg taaaggaatc gccgacggcg agaatgcggc atatctgagg ggtgggctgc
     5221 tgatattctt gatcttcgcg gagcccctgg ccgttgatac gtacttgcgt gcggaactcc
     5281 ggatatcgga atgtgccgga gaaatgcggg cgcagtttgt attgccggct tgcggagggc
     5341 acataaagac cctcgggata ccgcgcgaga atctggggac gggccagacg cacgactcct
     5401 tcgccggcca gcaaacctat tgccacgccg gctcctgcgc ccaacacggc cttggccagc
     5461 ctgcggctct gaccaacgag cttcggccag aagactcgcc agagaaatcg gggaatgcca
     5521 cacagatacc gccggcgttt gtgtggctgc tgggtgagca actggagcca ctccaggccg
     5581 agcctgcgga cccagaccgg cgcgcgtcga agttcgcccg ccagatgatc aaacaggccg
     5641 cctacgccga tggccgcctt gcagccaagt tcgtgcaatc gccggtcgat aaattcttcc
     5701 tgccgggggt tgcccatccc gaccagcagc aggtccggct tgaagcggcg aacctggttg
     5761 acgatgtccc gctccgcgcc gggcgaaaaa tatccatggt gaaagccaac cacggagatg
     5821 gctggatatt cccgctcgag gcgagccgcg gcccgttgcg cgacgccagg ccttccgccc
     5881 agcaggaaga ggcgcagttg gttttcccga gatcgctcct gaaagagttg gggaagcagg
     5941 tccgtaccca cgaggttgtc aggaaacggc gccccagccg ctcgccctgc ccatctcacg
     6001 cccacgccgt cgttcagcac caggtccccg cgattcaaaa ttgcccgata ctcggcattt
     6061 tcaaaagcca gattcgccgt ggctgcgtgg acgaaataaa cgctatgggg cccgcgcccg
     6121 gcgagcatct tccgcaagca atccagcgct tcgcgctgtg tgcgccggcg aatgcggatt
     6181 cccaagatgt tggcttccaa tagcccttcc atagcgtcac agtcagctag caccggcccc
     6241 tgtccacagg gccggaagcg tgcgcagcaa gatcttcaga tccagcagca gcgaccagtt
     6301 gtcgatgtag tgcaagtcca gccgcatcca atcctcgaac cccagccggt gccggcctcc
     6361 tgcttgccaa aggcaggtga tccccggccg taccgaaaag cggcgccgct gccagccgcc
     6421 gctcagacgc tcgacatccc ggaccggcag cggccgcgga cctaccagcg ccatttcgcc
     6481 cttgagcacg ttgaggagct ggggcaactc atcgagactg gtgcgccgga gccagcgccc
     6541 tacccgcgtg atacgcggat cgttgcgaat cttgaatgcg gcgccgagta cttcattcag
     6601 gggctcaagc tcggcctgcc gggcctcggc atcaacggtc atggtccgaa atttccacat
     6661 gagaaaccgc cggccgttgt agcccacccg cacctgccga aaaagggctg gccccgctga
     6721 atcgagccgg atcaacaccg caatgatcgg ccagacagga agagtggcca gcaatccggc
     6781 ggcggctaga actacgtccg caattcgctt cagaaggaag ctccaggccg agcctcggac
     6841 tgtggaaagc gtcaggacgc ccagctcatc cagccgctcg gattccacct gcgccagagg
     6901 cgcctcaaat aaccccccgg gcaggcggat gacgatccct tgctcttcgc agaacgaaac
     6961 gagcctggat gcttgggaat aaaaactctt cagtggaagc gcgagggcaa cttcatcgat
     7021 ctgctcctcg cgcagaatgc gaggaagatc gccgatgccg cccagcagcg gcgccggacc
     7081 agggccgccg ccgctgtcgt ccagaaaccc cactacgcga tagcccagtt cgggccgcct
     7141 ttggatcgat tcagcgaaac ttcgcccccg gcgtccggcg ccgataatca atatgcttcg
     7201 gagattgcgc cctcgccggc gcaccgcggc gagactggtg tatagaaaaa cccgcgaaaa
     7261 cgcaaccaac gtcagggcgg ccgcccagaa ctcaagcaca gatgcgccat gaaaagcgct
     7321 ttcaccgaac gcagccagca ggcctccgag cagcagcgcc gccagtgtca cggcccgcag
     7381 aatggctttg tagaattttt tccggctccc gagccgctgg gactgataca gtccacagca
     7441 ttcgaagatg cggcggcaca gaaccagaaa aacgaacgcc agcaggacgc tgcgcaggct
     7501 gatgcgcagc gaaaggacgc cggccaggcc ggttgggcgc gctggcgccg ccggaagaag
     7561 cacggcgaat cccaagcacg cggacagcgc cgccaggtca aagatcttca acccttctgc
     7621 caacaagctc cggcgaaagt ggtgcatagg ttctgtgcca aacgccgccg cggtccagcg
     7681 ttgccggccg gcgcgtcctt tgtcatccga gccgccggta gagcggctcg ggaacgaaga
     7741 acttgcgctt gttgaggacg gcgccagcca cgctcgaatt cgcccgccgc agtcttgtcg
     7801 cggcattttg aatcacttct cggctcgcaa ggtccgcttc cactaccagc accgcggcat
     7861 caaagaaagg cccgaggatc agcgcatcgg aggtgttcac cggcggcaga tcaccgacaa
     7921 tcgccgggaa ccgctgccgc aagcgaaaga aaacgccgcg aaaattcgtc caatcgggca
     7981 agccggccag accgttcccc gccgagcctg cgggcaacag ccaaaggcga tcaaaggccg
     8041 tctggtgcag gcaggcctcc aaggggaact gctcgtcgtg aaggatttca cgtagtcccg
     8101 ggctcccgct gagaccaaag cgcccggctt gtgaaggcct gtaaaggttg gcctccaaca
     8161 gcaatgttgg gtttccggtg gcccgggcag cctgaaccgc caaatgaaag gccacgtaac
     8221 tggtcccttc gccggacgta gccgaagcca tcgcgattga acggggcaat cgggcggccg
     8281 gcggtaagaa gaggcggtag acaagctgct gccattgatc gcggtgctgc gtcccctgcc
     8341 ggctccacgg agatggcgaa gaaggggggg tggaaaccgc cggcgcggaa gaccgccgcg
     8401 cccctggttc ccgcggagtc gagaataatt caccctcgag tttcgctttc cggagaatct
     8461 ccagataccg gctcatattt gaccctctgc gtcattcctt gggataggca ggcagcagca
     8521 gaacttcgcg cgctttcagg ttgtcagggt ctgtgagctg cggattggcg cggcggatcc
     8581 attgccagat ctcctcgttc cattggccgt attcgcgcag cgcaatcctt cggagcgttt
     8641 cccctggcgc cactgaaacg cgggctgcgg gaaatgtttc cggcgtccgc tcggcgatcg
     8701 gtaattccgt ggcgggttgc tcttcggtcg gggactcggg cggccggtct gacccttcgg
     8761 cggatcggga tgcggccggc gccgtttcgg gcagggccgc cggcgaggcg gcagccgggg
     8821 caaccaagat ctctccgggc tggggggtgc gaaagtggag cgcctgcttg accgccggca
     8881 gagtcgactc aaatcccatt gtcccggcca gcagcgctaa acttgcgaaa cccgcagccc
     8941 agcccaggcc cgccgccaga ctttttcggg gagccgtcgc cggaaacctc actgccgctg
     9001 ctgttgccgc cgaatgtatc tcctccctcc ccacccgctt cttacctttc agaaatgcag
     9061 ccgagagagc ctcgaagcac aaggtattga tcttccgagg aaccccgccg gaagcctcgg
     9121 cggccagttc tagagcgcgc cgggaaaaga tccttcgttt cacgccggca acgcgcaatc
     9181 ggtgcgagat gtagcgcgcc acatcctcgg aattgaggcg gccgataccc gcgtggaggc
     9241 agatgcgctg tcgcagttgt tcgcactctg gggattccag ccgctcggaa agcgccggct
     9301 ggccggccaa cacaatttcc atcaattttt cctgcggcgt ttcgaaattc gagagcaacc
     9361 ggatatcttc gagaaccggc gccggaattg cttgcgcctc gtcgaagacc agcagcagcc
     9421 gcctgccttg ccgatggcat tccaacgcga gagcttggag catctggcgc ttttcggaga
     9481 agtcccctgt acaggcgaga cccaaatcct ccagcacggc tgctatcatc tgatccctgt
     9541 tctcaggcgg gctgaacagg aaggccatct gtgcgcggtc ccgccagcgg tccatcaagt
     9601 agtggagcaa tgtcgtcttg cccatgcccg gctcggctgt caaaaccagc aagccccggc
     9661 gctcgcttag tccgtagtgc aaagccgcca gcgcctccgc atgctgggcg gtgaagtaga
     9721 gaaacttcgg atcaggagtg ctgccaaacg gctgttcccg caggccaaag tgttccacaa
     9781 acattctgcc cgctcacctc cttttcgact ccggtataca ggccagaacg gagagcgaag
     9841 tctgcgcttc cagttgcgcc ggcgtgtgga tggaatgatc cgccaattcg gctgcaaagc
     9901 cgcccgccgc cgcgactgcc gccgccaaaa gcacgctggc tgcaaaataa aaccacacgc
     9961 cccgggacgc gacgcgaaac ggcgggcggg ccggttcggc tacggaaacg ttcaatacgc
    10021 ctttttggtc gagagcctcc gcttcttccg cctcggtgta tttcttgcga tacagcatgt
    10081 agccttcctc ggcgatcttg acattgcggc tcaactccgc tttttcggaa gcgttttcgg
    10141 ctgcgatgag gcgcgaagcg gccaattcct cgcgggcctt gtgctcttgt tcggaaatgc
    10201 tggcgagccg cgattgcagc cctgccagcg ccgccttcgc ccgcaccaat tcgccttcaa
    10261 cggtctggcg taacggattc gggaggttct cttcgaaact cctgcggtgc gactgcgctt
    10321 cctggatctg ctgccggagt tcgccgatcc gcgtctcgag ccggtcgatt tcgcgataga
    10381 acttcgcttc ggaacgccgg ttctgcgcct ccaggagttc taattggagg cgttccagat
    10441 acgggttctc tccaatctga cgcgccgagc gaatcgtcac aggaatcaca gccagttcgc
    10501 gagtcaattc ggcgacccgg ctcctggctt ctcgaatggc gctttcggag tccgatttct
    10561 gcttttcgag atcggacagc ttttgtcgcg cccgctgcgc cacgtcgacg gccacaagct
    10621 ccggctgtcc cttctcaaat tccgccaggt ccgcttccgc ctgccgcaac tgctgctcga
    10681 aatcatccgc ctgcgaccgg taatattcca ccgcggcggg acggcgattt actgccacat
    10741 acttttccgc ataaatccgc ggcaacgcct cgagcacccg agccacctcg gccgggtcgg
    10801 gcgaactgta acggacagcg atcagattgg tgaagttgcg ccccgcagtt acgggttcga
    10861 ccgtaagacc ctgatccagc cgctcgggag aagccacacg cgcctcacgc gccagccggt
    10921 ccaaaacgtc ggagcttctg agaatttcga tttccgattg aatctcttgg ggcgcaaccg
    10981 ttgtattcag cgtgggaacg gcccggtcaa atggagtgac cacggcgtca aagcggtcct
    11041 ttctgagaat aaatcgaagc tccgacgcgt agcgcggcgg aaacaaacgc ggccgcaagt
    11101 aaatcgcccc aacagcagcc ccggccaaca cagatccaaa caacacgccc ataatcgctt
    11161 tacggcggaa aaatgcggcc gctaaatctc gcggccccgt gaccgcctcg ccccggcgta
    11221 gctccccggt gaaagggacc agatctacgc tgctgttttc acgtcgcaaa tgcgcacacc
    11281 gctccagctc atagccaagt tccagcggcg gaaccgccct ttactccagg tcaaaccgcg
    11341 tgggtgttgc gatgcgccga tcgcaatgga tcttggattc ctgacagaat gctgagctcc
    11401 cgaagattat atgcagtccc atatcggatg caagttggtt ttttcccaac gcattttcgt
    11461 aaaaatgggg gacaatacca accaaaatac tgtttttatg ttgccgaagc cgaaacgggg
    11521 cggcctcccg atgacggcgc tgtggcctgg gctgcccggg cagaccttcg cggcaaaatt
    11581 gggaaccgcg agccggccca atagcgctga gcgaaacgcg cggccgctgc gcgatcctct
    11641 gcgccgatct tggccagcag ccgccttacg tgccaccgca ccgtctcatt gctgatgaac
    11701 agggagtttg caatttcccg gttggagcgg ccctgggcga tcagctcgag gatttgctcc
    11761 tcccggccgg tcaattgcga gcttgcgccg ccgccgtcca gcaaatgccg caataaatcg
    11821 gccacctgcg accggggagc ccaaaattgt ccggccgcca ctgcgtaaac cgcccttgtt
    11881 atagtggcca gggtctcgct tcgatcgaga tatccgagac agccgagctg gagccaggac
    11941 atcgcttctt cgggggcgcc gcccgatcct atgaccagca ccgatatcgc gcgcccgtaa
    12001 tcgatcagtt cccgaaaacg ccggggatcg atgctgtcga gaaacgagtc gccgataatg
    12061 agcacgcagg gatggaatcc ggcgcactgg ttcagaacct cctgcggaga gccagttgcg
    12121 actctggcca agacgtcggg ctgttcccgc aggcattgtg tgatcgcttc gccggtcagt
    12181 tccagagagg acgcggcaaa aacatgaata ggcatcggcc agctccttac gttggcgtca
    12241 aaaaacgtta ctaagcctac tccataaagc cggttgttgg gaagtctgtt ctgtttgttt
    12301 tttaaaaaaa gaattattca aaaaatatgg aattattttt tgggaaacgt gataaattac
    12361 cgcatgcaag cctggtttgc ctgctccgcg gccaggttcc cgagctgacg gcagggagct
    12421 cggcctacat tcctccaaaa aacaagatgc gctggcagaa aatcatcgcc ggttggcaat
    12481 tgggagctgc gctggcgtgc gctgcggagc tgagtccggt agccctccgc gtagccggcc
    12541 agctcaccaa tcgggccaac ggcttcaatc ttgtcgaggg ccgagagctg gccgggccgc
    12601 aaggcctggc aatcgatgct tcagcgacgc ccccgcacat ctacgtggct gacacgctga
    12661 acaaccgcgt cctggggtgg cgggacgccg gcaacttccc cacgggcgcc gtggcggacc
    12721 tggtcatcgg ccagccggat ttgttgggca cggggccaaa ctcgcccgat gtgtcggtag
    12781 gattctggtc tcccgccggc gtggccgttg acgccgccgg caatctgttt gtcgctgata
    12841 ccaacaacca ccgcgtcctc cggtatccac ggcctttcga ccaaaaaaat caagcgccgg
    12901 accttgtggt gggtcaaatg aatttcagtt cgcacagccg caatatgggt ttggccgatc
    12961 cgaacagcgc ttctctggcg tatccggtag cggtggccct caacgcgcag ggcgacctcg
    13021 ccgtttctga ttccgtaaac caccgtgtcc tcatttttcg cgctggatct ctggcggcaa
    13081 acgggcctgc cgctgacgtt gttttgggtc aggcgaattt ctcgacctcg gccggaggcg
    13141 ccgggccagc cggcatctcg cagccgggag gcgtcgcttt tgacggaacg ggccggttat
    13201 acgtggccga tgttaccggg aatcgggtgc tggtgttcag cgccggcgcg gccaccggag
    13261 ccagcgccgt tcgcataatc ggcggcaacc cggatggcgc cttgcgcggc ccgcacacgc
    13321 tgtaccggcc gttcggcgtc gctgcggccg gcgatatgct tctggtggcc gacacgggca
    13381 atcaccgtgt cctgggcttc cacggattga gcgccgccgc gggagccgtc gcagcggccg
    13441 attttgtttt cggccaaccc gatttcacaa gcgcactgcc gaatgcggtg agcgaggccc
    13501 ggccgctcgc ttcggcgcgg gcgctctgtt ctccggcgca agcggcgctc gggccggacc
    13561 gcacgatgtt cgtggccgac gcgggcaaca accgcgtact cgccgtgccg ccggccgggg
    13621 cggcctacgt ccgggcggcg cgtgtgctgg ggcaagcggg cttcacgcaa aatgctccca
    13681 accgggtgga tggccgcg
//
LOCUS       DASREK010000087        12192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_381147, whole genome shotgun sequence.
ACCESSION   DASREK010000087 DASREK010000000
VERSION     DASREK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12192
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381147"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFA54_04595"
     CDS             complement(<1..443)
                     /locus_tag="VFA54_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZS50116.1"
                     /translation="MEQVWFVAALWLGLALVATLLAIWFRVSTALSEIVVGTVAQLLI
                     GALAGAEALGVKSAWITFLAGTGAIVLTFLAGAELDPSTFRARWKEAGTVGLVGFLGP
                     FLGCSAVSYFFLGWSGRASLLTGIALSTTSVAVVYAVMLELGFNRT"
     gene            complement(610..1527)
                     /locus_tag="VFA54_04600"
     CDS             complement(610..1527)
                     /locus_tag="VFA54_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50117.1"
                     /translation="MPDAEIQQLIEELHSLRDGEQAVIRLISLGWKAVGPLREFLLQS
                     APVSVYQPRRWAVEALAALGAKDVLIEYLLREDKVSDPVVRLGEEAVENAAARALAEW
                     QDVEAFLALSMVSERKLLAGVVDGLGRFGRMESIPYLQRALEDDLCREAAEAALRNMG
                     AAARPSLIASALIPRPSMGEESPSSVRRRRSALNILGEMGMGDEDRKALFPLLNDADP
                     EIVIRAATILEPGAAGEQRRQMARRLLEVLPAADWTFREDLESCLQRLFADARLEIEQ
                     EIARRMALPAKDRILDFALRVLLRVRSRC"
     gene            complement(1520..2797)
                     /locus_tag="VFA54_04605"
     CDS             complement(1520..2797)
                     /locus_tag="VFA54_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS50118.1"
                     /translation="MGSELHPSGAKILSGEPQNLRAARQRRPAWLNRNVAGMSVTSFF
                     SDAGHEMVTAVLPGFLGVIGVPAGVLGWIEGGSDAAASFVKLGAGWYSDRIGSRKAMV
                     TAGYFLTGTALALFAAAVSWPLILLGRLIAWIGRGIRAPLRDAMLSDSVSPDARGKAF
                     GLHRAGDTLGAVIGPLIGVGCLSLLPASSASGPFRIIFLFSLVPGLASVAAFAGLVRE
                     NKSRGQKGLRFWGALSNLPYPYRRFLTGVGVFGLGDFSHTLLILAATQLLAPERGPVA
                     AAQWAAGLYVIRNLFYAGASFPIGFLADRISKTKLLAAGYLLGACTAMATSALFVFHA
                     SRSGALAAVFAAAGFYIAMEDALEGAIPADLVPSGLRGVGYGVMGTVNGVGDLAASAL
                     VGTLWTAVSPAAAFGAAALLMLLGAALVLRNHA"
     regulatory      complement(2805..2871)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     regulatory      2945..3011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            3035..5056
                     /locus_tag="VFA54_04610"
     CDS             3035..5056
                     /locus_tag="VFA54_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HZS50119.1"
                     /translation="MNSLNAAQKRHLLSELKHADTLLSDIEAVLAASASKAAFPKYKQ
                     DLSPRQIKVIQDYIARLRTQMLRAAEGFDLDRSEPAFGSLHSIRTILTFIDIAFEELR
                     PKYMRGYGAVPESAAPELNGLADELQGIASRLDAYLAAGLGQDIPARLERLAQAGTDT
                     SLLSRLEQVISDHGLVEFRPALSIILERLENNALEIALFGRVSSGKSSLLNRILGVDV
                     LPVGVNPITAVPTRIVYGPAAVLTVWFADRRPERCDISRLAEFAAEQHNPANARHVTK
                     IVVELPASGLCEGVTFVDTPGLGSLAAAGALETRAYLPRCDLGVVLVDAGSTLTQEDI
                     GTIHALYEAGIPASVLLSKADLLGPGDLSQAEQYIAAHIASELGFNLRIHPVSVIGEH
                     AALLDRWVEQEIRPLYSRRRELAAESVRRKLGALREAVEAALRARLQRAGRSSAAGAE
                     VAKKIESRLRRAAGKLQQTRKICEKLAGEAASLANSVVARAAVHALRTAGIDAPAAVG
                     AALDEVTGEITKEIRQALETLAKELAGALESTAADLGSPGWAEEGLDTIIREMPRPDL
                     GQIHLDLRVSVLSKFAKNWEIRRMQSRIRNQIGSTIDAALDAYSRLLESWSRKTIAEM
                     QRRFDAQADSYRAEAGRIAGAEEASAQDPEAIRRSLESIQAPVAAGHRE"
     gene            5061..5558
                     /locus_tag="VFA54_04615"
     CDS             5061..5558
                     /locus_tag="VFA54_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50120.1"
                     /translation="MYATARTPTNTRWYRFVYEFQSAAEAPVELVEVLQRRSGWSHAV
                     FFPRMEAHSGQPVSPPSVCVLHEDRLCLYRIGDNWDVACQDIPLPSIDRIVLQLYLLA
                     CSIEIFWPGGSARLRFPAYGAEPVVALSRRLTGPDWEKTVMREPVLGYGVEISWRRPG
                     SLPKS"
     gene            complement(5624..8494)
                     /locus_tag="VFA54_04620"
     CDS             complement(5624..8494)
                     /locus_tag="VFA54_04620"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HZS50121.1"
                     /translation="MEQVTVDPSSKLADAAGGTTTAQRQKQGVVFPRYFTSHLAEGRS
                     PYDEIEWERRTASIGNDKGAVIFEQRDVETPRFWSQTATNIVASKYFHGKLGTPERET
                     SVKQLVHRVVDTITEWGLAGGYFATRQDGENFRDELAHLMLHQKACFNSPVWFNVGVR
                     EARGYGWAWDAQQGRVVALEKEGQRPQCSACFIVSVKDTLESILDLAKTEGMLFKWGS
                     GTGSNLSTLREEDAVLSGGGRASGPLSFMKGFDAFAGVIKSGGKTRRAAKMVILNADH
                     PDIEDFIWCKAKEERKAWTLVEAGYDASIDGDAYSSIFFQNANNSVRATDDFMLAAIN
                     DGDWWTRSVVSGQPKDRYKARDLLRQIAEATHQCGDPGMQFDTTINRWNPCKNSGRIN
                     ASNPCSEYMFLDDSACNLSSLNLMKFMTAAGQFDVEAFRRAVDTMILAQEIIVDNASY
                     PTAKIAENSHTFRPLGLGYANLGALLMSMGLAYDSDQGRDTAAAITAVMCGQAYLTSA
                     RIAEAYAGGIGPFPGYEANREPFLDVIRMHRDAVSRIDRRNISEPLATAAQACWDEAL
                     ELGRRAGYRNAQVTVIAPTGTIGFMMDCDTTGIEPDLALVKYKKLVGGGVIKIVNNTV
                     PSALLKLGYSPEQVSDIVSYIDATGTIEGAPHLKSEHLPVFDCSFRPQNGVRSIHHMG
                     HIKMMAAVQPFISGAISKTINVPEECTVEDIMDAYIQSWKLGLKAVAIYRDNSKRVQP
                     LSASKHDKKEVGKGAAAAPAVQTVVTEKIVYRPTRTKLPDERKSITHKFSIAGHEGYI
                     TVGMYEDGAPGEIFIRMAKEGSTISGLMDSFATAVSMALQYGVPIKVLCDKFVHVRFE
                     PSGYTNNPEIRYAKSVMDYIFRWLGLKFLGPQYGNQAVEAEEVASASELFPTEPEPQQ
                     KLPFRPAPTVEDAPTCSECGSIMTRNGSCYKCANCGSTSGCS"
     gene            complement(8978..10225)
                     /locus_tag="VFA54_04625"
     CDS             complement(8978..10225)
                     /locus_tag="VFA54_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZS50122.1"
                     /translation="MATSATETNTAAENTIDLEKQYVLQSYARYPLVIEWGKGAVVRD
                     EKGRRYLDFISGIGVNALGHAHPRIVKAVREQVGKLVHCSNLYYHRYQGPLAKRLAEI
                     TGLQRTFFCNSGTEAIEGALKIARAFGGKQNSRKHEVVSLENSFHGRTLGALSVTGQT
                     KYRQDFEPLLPGVRFVPPNDSEALEQAVGENTAAIVLEPILGEGGIVALSREFAQHAA
                     SLARRYNALLIFDEIQCGLGRTGAYFAFQLWNQGRPAEAAITPDVLVTAKPLGCGFPI
                     GAIVASERAAAAIGSGMHGSTFGGGALICRVALEFLDILDGLLPAIRENGRYFRERLR
                     MLAEKHKIVRDVRGEGLMLGLDLAVPGKTLVLEAMERGFLINATHDTVLRFLPPYVIE
                     QKQISRLIAALDEILGTVPDALT"
     gene            10413..10970
                     /locus_tag="VFA54_04630"
     CDS             10413..10970
                     /locus_tag="VFA54_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50123.1"
                     /translation="MLRLGLLAFAGAGLLFGQPSPGRELKGVIYWPAAELKRFSAALK
                     AKEPAVHADRRSMNAFDYLGHRGNYTVLEGRRDESSVPEVHAGADDIFLIQDGEATML
                     YGGVVEGAARVQKGAMRGARLAGAQSIRMNPGDVLIVPGDVPHQMIVEKGKWITFFTI
                     KIESRQPGRPAAGSRLQPASPKQKP"
     gene            11034..>12192
                     /locus_tag="VFA54_04635"
     CDS             11034..>12192
                     /locus_tag="VFA54_04635"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZS50124.1"
                     /translation="MFFRDRFNVTQRDLERYLSEALSRGGEYADLYFEYIATCSLSLD
                     ESLVKSAAQGVSAGCGVRVLSGEKTGYAYTDDLSPEKILQAARVAAQIAAAPSRAQSF
                     GISEQAAVHDLYPVLSTDEELAAKLDLLHRADRAARRFDPRVFQVRAGYADERRHVLI
                     ATSAGRVAEDFQPLTRLSVVALAKQGASVQRGTDGGGGRVSLDFFEKIRTPEQFAREA
                     ARQAVVQLEALDAPAGEMTVVLGPGWPGILLHEAVGHGLEADFNRKQVSAFAGLMGRQ
                     VASRLCTVVDDGTIPGRRGSLNVDDEGNPTNRTVLIEKGILKGYLQDTLSSRLLGSAP
                     TGNGRRESYRHMPMPRMTNTFMLPGEDAPEDIIRSVPRGLYCATFGGGQVDI"
BASE COUNT         2344 a         3811 c         3741 g         2296 t
ORIGIN      
        1 gtccggttga atccgagctc cagcatcact gcgtagacta cggccaccga cgtggtcgaa
       61 agcgcgatgc cggtgagcaa gctcgctctt ccggaccatc ctaggaagaa gtaagaaacg
      121 gcgctgcacc cgaggaacgg acccaagaag ccaaccagtc caacggttcc ggcttctttc
      181 caccgcgcgc gaaaagtgga cggatccagt tcggcgccgg ccagaaacgt caagacaatg
      241 gcgccggttc ctgccagaaa cgtgatccac gctgatttca cgcccagcgc ctctgctccc
      301 gccagagcgc ctatcaacaa ctgggcgaca gttccaacga cgatttcgga aagcgcggtg
      361 gagacccgaa accatatcgc cagcaaggtg gctaccagag ccaacccgag ccacagggca
      421 gccacaaacc agacctgctc catgtccgat ctccattcct attgattttt ccagggaacc
      481 gctgtcgaag cgtctttcag gagggcgacc cccacagcga ttccactgta ggagtcattg
      541 gccgggacgc gccggcgatt gcgcggactc caccgctatc tttaagcata cagaaaacgc
      601 cttcaggatt caacagcgtg agcggacgcg caaaagcacc ctcagtgcaa agtcgagaat
      661 ccggtctttc gcgggcagag ccatccggcg ggcgatttcc tgttcaatct ccaaccgggc
      721 atccgcgaag aggcgttgga ggcaggattc gaggtcctcg cgaaatgtcc aatccgccgc
      781 aggcagcacc tccagcaggc gccgcgccat ctggcgccgc tgctctccgg cagcccccgg
      841 ctccagaatc gttgccgcgc ggatcacaat ctccgggtcg gcgtcgttta gcagagggaa
      901 cagcgccttc cggtcctcat cccccatccc catctctccg aggatgttca atgcgcttct
      961 gcgccggcgt acgctggagg gcgattcctc ccccatgctg ggccgcggta tcaacgctga
     1021 ggcaatcaac gagggccggg cggctgcgcc catatttcga agagccgcct cggcggcttc
     1081 gcggcatagg tcgtcctcga gagcccgttg caggtaggga atcgattcca tacggccgaa
     1141 tctgcccaga ccatctacga cgcccgccag cagcttcctc tcggaaacca tgctcaatgc
     1201 caggaaggct tcgacgtctt gccactccgc cagggcccgc gccgccgcgt tctcgacggc
     1261 ctcttcgccc aggcgaacca cgggatcgga aactttgtcc tcccgaagca gatactctat
     1321 gagcacgtct ttcgcaccca gggcggcaag cgcctcgaca gcccatcgcc gcggctgata
     1381 gacgctcacc ggagcgctct gcaaaagaaa ctcccgaagc gggcccaccg ccttccaccc
     1441 gagcgagatg agacggatga cggcttgttc cccatcgcga agcgaatgga gttcttcgat
     1501 aagctgctgg atttccgcgt caggcatgat ttctgagaac cagcgcggct ccgagcagca
     1561 tcaggagcgc cgcggccccg aaggcggccg ctggggatac cgccgtccat aatgtgccta
     1621 ccagggcgct ggcggcgaga tcgcccactc cattcacggt gcccataacc ccgtacccta
     1681 cgcctcgcag cccgctcgga acaagatccg ccggaatcgc tccctccaaa gcgtcctcca
     1741 tggcgatgta aaagcctgcc gctgcaaaga cagcggcgag agccccggaa cgcgacgcat
     1801 ggaaaacgaa cagcgcggat gtagccattg ctgtacaggc gcccagcaga tatccagcgg
     1861 ccagaagctt cgttttcgaa atccggtccg cgagaaagcc gatcggaaag gaggctccgg
     1921 catagaaaag gttccgaatg acatagaggc ccgctgccca ctgtgcggcg gccacgggac
     1981 ctcgttccgg agccagcaat tgtgtcgctg ccaggatcag aagcgtgtgc gaaaaatcgc
     2041 cgaggccaaa cacgcccacc cctgtcagaa accgcctgta ggggtatggc aggttgctca
     2101 aggcgcccca aaaccgaagc cccttctgcc ctcgactctt gttttctcgg accagcccgg
     2161 caaaggcggc gacagaggcg agacccggaa cgagggaaaa aaggaagatg attcggaacg
     2221 ggccggacgc tgaggaggca ggcagcaggg ataaacagcc caccccgatc agcggaccga
     2281 taacagcgcc cagagtgtcg ccggctcggt gcaggccgaa ggccttgccg cgcgcatccg
     2341 gagagacgga atcggaaagc atcgcgtccc gcagcggagc gcggattccg cgccctatcc
     2401 aggcgatcag tctgccgagg agaatcagcg gccacgacac tgcggcggcg aacagcgcca
     2461 gggccgtacc agtcaagaag tagccggcag tgaccattgc tttccggctc ccaatccggt
     2521 ccgagtacca acctgctccc agcttcacaa agctcgcggc cgcgtccgag ccgccctcaa
     2581 tccatccgag caccccggcc ggcacgccga tgacgcccag aaaacctggc agtacggcgg
     2641 tcaccatttc gtgtccagcg tcggagaaga agctggtcac gctcatgcct gccacattcc
     2701 ggttcagcca cgccggccgg cgttgccggg cagctctcag attttggggt tcgcctgaaa
     2761 ggatcttggc gccggatgga tggagttcac tgcccacctt gttcctcctt gtaggagtca
     2821 tctgccccgg gaggggcgtt ttaaagggtg aactccaaca cctgtatttc gattatgccg
     2881 cccgcgcggc cgttcgtcaa tcacgagccc agaccgttga cactccggcg ctgcacaacg
     2941 gaaaataaac gtaggtgttg gagttcaccg attaaccgcc ctgtaaaagg gaagataact
     3001 cctacccagc tagcttgctc ctggatacga gacggtgaac tccctcaacg cagcccagaa
     3061 gcggcacctc ctctccgagc tgaagcacgc cgatactctg ctctccgaca tcgaagctgt
     3121 gctggcggct tccgcttcga aggcggcgtt tccaaagtac aagcaggatc tctcgccccg
     3181 gcagatcaaa gtaatccagg actatatcgc ccgcctccgc acccaaatgc tccgtgcggc
     3241 agaagggttt gacctcgacc gctccgagcc tgctttcgga tcgctgcact cgattcgaac
     3301 gattctgaca ttcatcgata tcgctttcga ggaattgcgg ccgaaatata tgcgcgggta
     3361 tggggccgtg ccggagtcgg ccgcgcccga actcaacggg ctggccgacg agctccaggg
     3421 gatcgcctcg aggctggacg cgtatctggc cgctggtctc ggccaggaca tccccgcgcg
     3481 tttggagcgg ctggctcagg ccgggacgga tactagcctg ctctcgcgtc tggagcaggt
     3541 gatctccgat cacgggcttg tggagtttcg tccagccctc tcgatcattt tggagcggct
     3601 cgagaacaac gccttggaga tcgccctgtt cggccgggtc agctccggca agtcttctct
     3661 gttgaaccga attctcggcg tggacgtgct gccggtgggc gtcaacccga ttaccgccgt
     3721 gccgacccgg atcgtgtacg ggccggcggc ggttttgaca gtctggtttg ccgaccggcg
     3781 gccggaacga tgcgacatct cgcggctggc cgagttcgcc gccgagcagc ataacccggc
     3841 aaacgctcgc catgtgacga agatcgtcgt cgagctcccg gcgtccggtt tatgtgaggg
     3901 cgtgaccttc gtggatacgc cgggcctggg atccctggcc gccgccggag cgttggagac
     3961 gcgcgcctac ttgccgcgat gcgacctcgg tgtggtgctg gtggacgccg gctcgacgct
     4021 cacccaggaa gatattggta cgatccatgc cctctatgag gcaggtattc ctgcgtccgt
     4081 gttgctgagc aaggcggatc tgctaggccc cggcgatcta tcgcaggccg aacagtatat
     4141 cgccgctcac atcgcatccg agctcgggtt caatctccgt attcatccgg tcagcgttat
     4201 aggagagcac gccgctcttc tagaccgttg ggtggaacaa gagatccggc cgctttacag
     4261 ccgccgtcgg gaactggcgg cggaatcagt ccggcggaag ctgggggctt tacgggaagc
     4321 ggtcgaagcc gctctgcgag cgagattgca gcgggctggc cgttcttccg cggccggggc
     4381 cgaggttgcg aagaagatcg agtcccgcct gcggcgagcc gccggcaaac tgcaacagac
     4441 gcgcaagata tgcgaaaagc tggccggcga agcggccagc ctcgccaaca gtgtcgtcgc
     4501 aagagccgct gttcatgcgc tccgcactgc cggcatcgac gcgccggcgg ccgtcggtgc
     4561 ggcgctggac gaagtgactg gcgagataac caaggaaatc cggcaggcgt tggaaaccct
     4621 ggctaaagag ctcgcgggag ctttggaatc caccgcggcc gatctggggt ccccgggctg
     4681 ggccgaggag ggcttggaca cgatcattcg tgaaatgccg cggccggatc tgggccaaat
     4741 ccaccttgac cttcgtgttt cggtcttatc gaaattcgcc aaaaactggg agatccgccg
     4801 gatgcaatcg agaatacgca atcagatcgg ctctacgatc gacgctgcgc tcgacgcgta
     4861 cagccgctta ctcgaaagct ggtcccgtaa aaccatagcc gagatgcagc ggcgcttcga
     4921 cgcgcaggcg gattcctatc gtgccgaggc cggccgcatc gccggcgcgg aggaggcttc
     4981 tgcccaagat cccgaggcga tccgccggag cctcgaatcg atacaggccc cggtcgccgc
     5041 cggccaccgg gagtagcaaa atgtacgcca cagctcgaac gcctaccaac acgcgctggt
     5101 accgttttgt gtacgagttt caatcagcgg cggaagcgcc ggtggaactc gtggaggtgc
     5161 tccaacggag atccggctgg tcgcacgcgg tgttcttccc cagaatggag gcgcactccg
     5221 gccagcccgt ttcgccgccc tcggtttgcg tcctgcatga ggatcgcttg tgcctctacc
     5281 gcatcggaga taactgggac gtcgcctgcc aagatattcc gctgccgtcg attgaccgga
     5341 tcgtgctcca gctctattta ctcgcctgct cgatcgagat tttctggccg ggaggcagtg
     5401 cgcgcctccg ctttcctgcg tatggggcgg agccggtcgt tgcgctctcg aggcggctga
     5461 ccggcccgga ttgggagaag acagtgatgc gggaaccggt tctggggtac ggagtcgaga
     5521 tcagctggcg gcggcccgga tcgttgccga agagctgaag tcggcctgaa agggccgctt
     5581 gcccgcttga cctgcgactt tgtgcgggct gcccgggcgg caatcagcta cacccgctcg
     5641 tgcttccgca gttggcgcac ttatagcagc ttccgttgcg cgtcatgatc gagccgcatt
     5701 cggaacacgt tggggcgtcc tcgacagtag gcgccgggcg gaaggggagt ttctgctgcg
     5761 gctccggctc agtcgggaat agttccgaag cgctcgctac ttcctccgct tcgacagcct
     5821 ggttgccgta ctgggggccg agaaacttca gcccgagcca gcggaagata taatccatca
     5881 ccgacttcgc gtaacggatc tcaggattgt tcgtatagcc gctcggctcg aaacgtacgt
     5941 ggacaaactt gtcgcacagc accttgattg gcacgccata ttgcaacgcc atcgagacgg
     6001 ccgtcgcgaa gctgtccatc aggcccgaga tggtggagcc ttccttggcc atgcggatga
     6061 agatctcgcc cggggcgccg tcttcataca tgccgaccgt gatgtagcct tcgtggccgg
     6121 cgatggagaa tttgtgggtg atggatttgc gctcgtcagg cagtttcgtc ctggtgggcc
     6181 ggtagacgat tttctcggtc acgaccgtct gaaccgcggg cgccgcggca gcccccttcc
     6241 cgacttcctt cttatcatgt tttgaggcgc tcagtggctg gacccgcttc gagttgtcgc
     6301 ggtagatggc cactgccttc aaccccagct tccagctctg gatgtaggcg tccataatgt
     6361 cttccaccgt gcactcctcg gggacattga tggtcttgga aatggcgcct gaaataaagg
     6421 gctgcaccgc cgccatcatc ttgatgtgcc ccatgtggtg aatggagcgc acgccgttct
     6481 gcggccggaa gctgcaatcg aaaaccggca agtgctcgga tttcagatgc ggcgctccct
     6541 cgatcgtgcc cgtggcgtcg atgtacgaaa cgatgtcgct gacctgttcc ggcgaatagc
     6601 ccagcttcag gagcgcagag ggcaccgtgt tgttgacgat cttgatcacg ccgccgccga
     6661 cgagcttctt gtatttcacc agcgccaaat cgggctcgat gccggtggtg tcgcaatcca
     6721 tcatgaagcc gatggtgccg gtcggagcga ttacagtaac ctgcgcgttg cggtagccgg
     6781 cccggcgccc cagctcgaga gcctcgtccc agcaagcctg cgccgccgta gccagcggtt
     6841 cgctgatatt cctccggtcg atgcggctta ccgcgtcccg gtgcatgcgg atcacgtcga
     6901 gaaacggctc gcggttggcc tcatagcccg gaaacggccc tatgccgccg gcgtaagcct
     6961 cggcgatccg cgcgctggtt aaatacgcct ggccgcacat cacggcggtg atcgccgccg
     7021 ccgtgtcccg cccctgatcg ctgtcgtaag cgagacccat ggacatcaac agagcgccca
     7081 agttggcata accaagacct agaggccgga aggtgtggga attttccgcg attttggcgg
     7141 tcggatagct cgcgttatcc acgatgatct cctgcgccag aatcatcgtg tccacggcgc
     7201 ggcggaacgc ctcgacgtcg aactggcccg cggcggtcat gaacttcatt aggttcagtg
     7261 aggaaagatt gcaggccgag tcgtcgagaa acatgtactc cgagcaggga ttcgacgcat
     7321 tgatccgtcc gctgttcttg caggggttcc accggttgat cgtggtgtcg aactgcatgc
     7381 cggggtcgcc gcactggtgc gtggcttcgg cgatctggcg cagcaaatcg cgcgccttgt
     7441 aacggtcttt gggctggccg gaaacgaccg agcgcgtcca ccaatcgccg tcgttgatgg
     7501 cggccagcat aaaatcgtct gttgcgcgca cggagttatt ggcattctgg aaaaagatgg
     7561 agctgtaggc gtccccgtca atggaggcgt cgtagccggc ctcgaccagc gtccaggcct
     7621 tgcgctcttc cttcgccttg caccagatga aatcctcgat atcgggatga tcggcgttga
     7681 ggattaccat tttggccgcg cggcgcgtct tgccccccga cttgatgacg ccggcgaagg
     7741 cgtcgaatcc cttcatgaac gaaagcggcc ccgaggcgcg gcccccaccc gaaagcacgg
     7801 cgtcttcctc gcgcagcgtg gaaaggttcg atccggtgcc ggatccccat ttgaagagca
     7861 tgccttcggt cttggcgaga tcgaggatcg attccagggt gtctttcacc gagacgataa
     7921 agcacgccga gcactgcggc cgctggcctt ccttttcgag cgccaccacc cggccctgct
     7981 gggcatccca ggcccagccg tagccgcgcg cttcccggac gccgacgttg aaccagaccg
     8041 gcgagttgaa gcaagccttc tggtgcagca tcagatgcgc cagctcatcg cggaaattct
     8101 cgccgtcctg gcgcgtcgcg aagtagccgc ccgccaatcc ccactccgtg atcgtgtcca
     8161 ccacgcgatg gacgagctgc ttgacgctgg tttctctttc cggagtgccc agcttgccgt
     8221 ggaaatattt gctggcgacg atgttagtcg ccgtctgcga ccagaaccgg ggcgtctcga
     8281 cgtcccgctg ctcgaagatc actgcccctt tgtcattacc gatagaggct gtgcggcgct
     8341 cccactcgat ctcatcataa ggagatcggc cttcggccag atgagaagtg aaatagcggg
     8401 ggaaaaccac accctgcttt tgccgctgag cggtggttgt cccaccagcc gcgtccgcca
     8461 gtttcgagct tggatccacg gtgacttgtt ccatcagatg tctctcccgg tcatgaaatc
     8521 gagccttcgc agcgtgcgga ggacatcata caatatcttg gggtcttgtc aagaattata
     8581 ataacatatt gtacaacgat ttgcgctggc gaggaagatt cgcccgcctt tccggccgga
     8641 tcagacgacc atcctgttcg acaattcctg gtggatggag aaggatcata gtggtttttc
     8701 ggcctgccag ccgcacgtgg ataacgccat gagggcgttg aaccgaatcc tgcctgtgct
     8761 cttcaccgca ggccacgggg ggccgggcag gtacagccag ggcctgacga tttgcttcac
     8821 gggctcggtg ggctttagac tatcgccgta gtgtagatgg ggaccgaacg cggcgtagcg
     8881 accggttttc cgtgcttcac aaaaaagacg gccctagagg gggtacgtgc gcctctgggc
     8941 ttcctacact cttaaaaaag aacccggccg gcggtagtta agtcagagca tccggtactg
     9001 tcccaagtat ctcgtccaga gccgcgatca agcggctaat ttgtttttgt tcaatgacat
     9061 acggtggcag gaaacgcaac accgtgtcgt gggtggcgtt gatcagaaac ccccgttcca
     9121 tggcttcgag gaccagagtc ttgcccggga cagccagatc gaggcccagc atcaacccct
     9181 ctccgcgcac gtcccgcaca attttgtgct tctccgcgag catccgaagg cgctcgcgga
     9241 agtagcggcc gttttcgcgg atggccggga gcaggccatc gagtatgtcc aggaactcga
     9301 gcgccacacg gcaaatcagc gcgccgccgc cgaaagtgct cccgtgcatt ccggacccta
     9361 tagccgcggc ggcccgttcg ctggccacaa tggccccgat cggaaagccg cagccgagcg
     9421 gcttcgccgt taccagcacg tcgggagtga ttgccgcctc ggccggacgg ccctggttcc
     9481 aaagctgaaa cgcgaaatac gcgccggtgc ggcccaagcc gcattggatc tcgtcaaaga
     9541 tcagcagggc attgtaacgc cgggcgagac tcgctgcgtg ctgggcaaac tcgcggctga
     9601 gcgccacgat gccgccctcg ccgagaattg gctcaagcac gatggccgct gtgttctcgc
     9661 ccacggcctg ctcgagcgcc tccgaatcgt tcggcggaac aaagcgaacg cccggaagca
     9721 gcggctcgaa atcctgccgg tatttggtct gcccagtcac cgaaagcgcg cccagtgtgc
     9781 ggccgtgaaa cgagttttcc agggaaacga cctcgtgctt ccgcgaattc tgtttcccgc
     9841 cgaaggcgcg ggcgattttt aaagccccct cgatcgcctc cgttcccgaa ttgcaaaaga
     9901 acgtgcgctg caatccggtg atttccgcca gccgcttcgc cagaggtcct tggtaccggt
     9961 gatagtaaag attcgagcag tgcacgagct ttccgacttg ctcgcgaacg gccttcacaa
    10021 tgcgcggatg cgcgtggccc agggcgttga cgccgatgcc ggagatgaaa tccaagtacc
    10081 ggcggccctt ctcgtcgcga acgacagcgc ccttgcccca ttcaatgacc agcgggtagc
    10141 gggcgtagct ctggagaacg tattgctttt ccagatcgat cgtattctcg gccgccgtgt
    10201 tcgtctctgt cgcgctcgtc gccatgaaaa gtatgatagc aaggcgcggt gcgccggcga
    10261 atcctcaggg gcgcgcgatt gccgcacatc ctcaaaacaa ggatcgccgc gggaggttcc
    10321 agcaacaggc ccggacgagg aaaggcgcgg cgccgtgtta ctcttcttcc tcgaagttct
    10381 tgccgaaaga tcgcctttag gaggagcgct ctatgttgcg actcggcctt cttgcttttg
    10441 ccggagccgg cttgttgttc gggcagccat caccggggcg ggaactgaaa ggtgtgattt
    10501 actggccagc ggccgaactc aagagatttt ccgctgcgtt gaaagccaag gagccggccg
    10561 ttcacgcaga ccgccggtcg atgaatgcgt ttgactactt gggccatcgc ggcaattaca
    10621 ctgttctcga agggcggcgc gatgaaagca gcgtccccga ggttcacgcc ggagccgatg
    10681 acatttttct gattcaggat ggcgaggcta cgatgctcta tggcggcgtg gtcgaggggg
    10741 ctgcgcgcgt ccagaagggc gccatgcgcg gggccagatt agccggagcg cagtcgattc
    10801 gcatgaatcc gggcgacgta ttgatcgttc cgggcgacgt tccccaccag atgatcgtcg
    10861 agaaaggcaa atggatcaca tttttcacca taaagattga gagccgccag cccgggaggc
    10921 ccgcggccgg gagccggctc cagccggcct cgccgaaaca aaagccgtga tctgccgatc
    10981 gctgcgcctt acctcccgga ctccgcgctg cccgcctacg ctacaattaa aatgtgtttt
    11041 ttcgcgatcg tttcaacgtc acccagcggg atctcgagcg ttatctgagc gaagcgctct
    11101 cgcgcggcgg cgagtacgcc gacctgtact tcgagtacat tgcaacttgt tctttaagcc
    11161 ttgacgaatc gctcgtcaaa agcgcggcgc aaggtgtttc ggcgggctgc ggcgtccgtg
    11221 tgctgagcgg agagaaaacc ggatatgcct acaccgacga tttgagcccg gagaagatat
    11281 tgcaagccgc gcgtgtggct gcgcaaatcg ccgcggcgcc ttccagagcg caatcttttg
    11341 gcatcagcga acaggccgcg gtccacgatc tgtatccggt gctttccact gacgaagaac
    11401 tggcggccaa gctggatctc ctccacaggg cggatcgggc ggcgcgacgc tttgatccgc
    11461 gcgtgttcca ggtgcgcgcc ggttacgccg acgaacgccg ccacgtgctg atcgcgacat
    11521 ccgcgggacg cgtcgcggaa gatttccagc cgctaacgcg cttgagcgtt gtcgcgctgg
    11581 ccaaacaggg tgcgagcgtc cagcgcggca cggacggcgg cggcgggcgc gtttcgctcg
    11641 attttttcga gaagatccgc acgccggagc agttcgcgcg ggaggcggct cgtcaggcgg
    11701 tagtccaact ggaagcgctc gatgcgccgg ctggcgaaat gaccgtggtg ctcggaccgg
    11761 gctggccggg gatcctgctg catgaagcgg tcggccatgg cctcgaagcg gatttcaacc
    11821 gcaagcaggt ttccgccttt gcgggcctga tgggacggca ggtggccagc cgcctgtgca
    11881 ccgtagtcga tgacggcacg attccggggc ggcgggggtc tctgaatgtg gatgacgaag
    11941 gcaatccgac caatcgcacg gttttgatcg aaaaagggat tctgaaaggc tacttgcaag
    12001 acaccctttc cagccggctc ctgggctccg cgcccacggg caatgggcgc cgggaaagct
    12061 atcgccacat gccgatgccg cgcatgacga ataccttcat gttgccgggc gaagacgctc
    12121 cggaagacat catccgctcc gtgccgcgag ggctttactg cgctaccttc ggcggcggcc
    12181 aggtggacat ca
//
LOCUS       DASREK010000088         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_52414, whole genome shotgun sequence.
ACCESSION   DASREK010000088 DASREK010000000
VERSION     DASREK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52414"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFA54_04640"
     CDS             <1..520
                     /locus_tag="VFA54_04640"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50125.1"
                     /translation="DSLARALETYRALVSDQDVPGRITFRTEQEKDTQALHQFEAVDR
                     DFHRSPEAQIARYYIGVVYYDLGRTADAEKQLQQVANEKQGNIPSLAKFTLAEVYLAE
                     GKDEEARKLYEFLLKNPTELVPETRVQLAMARYLRNKNPQEARNILLQLVKNQGAVSG
                     AAANMLRDLGAQ"
     gene            616..1737
                     /gene="dgt"
                     /locus_tag="VFA54_04645"
     CDS             616..1737
                     /gene="dgt"
                     /locus_tag="VFA54_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HZS50126.1"
                     /translation="MPALGHLRFPTEKTGGRRYPEAPHAYRNEYQRDRDRIIHSRAFR
                     RLENKTQVFTSPVSDHFRNRLTHTLEVSQIARTVACTLGLNEDLVEALALAHDIGHPP
                     FGHSGEARLDAEMRRFGDRFDHNLHALRIVESFEHRYAAFPGLNLTFEVREGIIKHSR
                     DYDAALFPELAEYRLCERPPLEAQLIDPADEIAYDCADLDDAVDAGLVGVEAVSEKVP
                     LFARFREQAERDCAGATADQKFHQALRQLLDAMVSALIEGTSAAAAIAKVRSAEDVRL
                     CPQRLAALTGEAQRAARDLKAFLYERVYNAPALLEEKQGSAAKLGMLFRYYLEAPEKL
                     PPAYQERALLEPLHRVVCDYIAGMTDNYLLRLYAAEITL"
     gene            complement(1727..>2992)
                     /locus_tag="VFA54_04650"
     CDS             complement(1727..>2992)
                     /locus_tag="VFA54_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HZS50127.1"
                     /translation="YPSGDQNKYSRYQGTGGFPVGSLLTRTAAAISFGEPNILLTGYL
                     TGESRMMIYRNIRPRLSHLAGFLLWDADPYMVITDEGRLVWLLDGYTTSRSHPYSATL
                     PVTGLAGGANYIRNSVKATVDAYDGRMALYVFDPADPIIQAYERLFPGLFRPASQMPA
                     DLRRHTRYPEALFRTQAEAYRIFHMRDPQVFYNKEDVWEIARDLYGHSGRPEAMAPTY
                     VVATLPGEANPEFMLILPFTPRGKDNLIGWMAGRCDGEHLGEAIYYQLPKQQLVYGPM
                     QIESRVDQDQNISKDLTLWNQQGSRVLRGNIIVLPVGNSFLYVESIYIQAVEARMPQL
                     KKVVLAMGNRLIYRDTFDEALADLTSGSPPQPPAGAVTSARPAQPPASQAQEPPANPA
                     LAEQLRKLHEQAQDLVRQIEALEKQAATK"
BASE COUNT          586 a          923 c          905 g          578 t
ORIGIN      
        1 cgactctctc gcccgcgcac tcgaaaccta ccgggctctg gtttcggacc aggatgtgcc
       61 cggccgcatc accttccgca ccgagcaaga gaaggacacc caggccctgc accagtttga
      121 agccgtagac cgggattttc accgttcccc ggaggcccag atcgcccgct actacatcgg
      181 tgtcgtgtac tacgacctgg gccgtaccgc cgacgccgaa aagcaattgc agcaggtcgc
      241 caatgagaaa caaggcaaca ttccctccct cgcgaaattc acgctggcgg aagtctattt
      301 ggccgaaggc aaggacgagg aagcccgcaa attgtacgaa tttctgttga aaaatccaac
      361 ggaactggtt cctgaaaccc gcgtgcagtt ggccatggcc cgctatctgc ggaacaagaa
      421 tccgcaggag gcccgaaata ttcttttaca gcttgtcaag aaccagggag ccgtgtcggg
      481 agcggcggcc aatatgctgc gggatttggg cgctcagtaa acaggggcca tggacggctt
      541 ggggaaagga aggcggcccc gcggcgggag gaacgcccct cccagggttt ccaatctttt
      601 gaatttctct cagttgtgcc cgccttaggt catctgcgct ttccaactga aaaaaccggg
      661 ggccgccgct atccggaagc tcctcacgct taccggaatg agtaccagcg ggaccgggac
      721 cggatcattc attcccgggc cttccgccgg ctggagaaca agacccaggt ttttacttca
      781 cctgtatccg accattttcg caatcgcctt acccatactc tggaggtctc acaaatcgcg
      841 cggaccgtgg cctgcaccct cggtctgaac gaagatctcg tggaagcact cgccctggct
      901 cacgacatcg gccatcctcc tttcgggcat tccggcgagg cccggctcga tgccgaaatg
      961 aggcgcttcg gcgaccgctt cgatcacaat cttcacgcgc tgcgcatagt ggaaagcttc
     1021 gagcatcgtt acgctgcatt cccgggcttg aacctgacgt ttgaagtccg cgagggcatc
     1081 atcaaacact cccgggatta cgatgccgct ctattccctg agctcgctga ataccggctc
     1141 tgcgaacggc ccccgcttga agcgcaactg atcgatcccg ccgatgagat tgcctacgac
     1201 tgcgccgatc tggacgacgc cgtggacgcc ggcctggtgg gtgtggaggc agtaagcgaa
     1261 aaggtcccgc tttttgcccg gtttcgagag caggcggaac gggattgcgc cggggcgacg
     1321 gcggatcaga aattccacca agccctgcgg caattgcttg acgccatggt ttcggccctg
     1381 attgagggaa cctcggccgc agcggccatt gcgaaagtac gctcggccga agacgtccgg
     1441 ctttgcccgc agcggctggc cgcactgacc ggcgaagccc agcgcgccgc tcgcgacctc
     1501 aaggctttcc tttacgagcg cgtctataac gcccctgcct tgctggagga aaagcagggg
     1561 agcgccgcca agctaggaat gctttttcgt tactatttgg aagctcccga gaaactgccg
     1621 cccgcttatc aggagcgcgc cctgctggag cccctgcacc gggtggtttg tgactacatc
     1681 gccggcatga cggacaatta tctgctccgc ctctacgctg cggagatcac tttgtagcag
     1741 cctgtttctc gagagcctcg atttgccgca ccagatcctg cgcctgctcg tgcagcttcc
     1801 gcaactgctc ggccagggcg gggttggctg gcggctcctg cgcctgggat gccggaggct
     1861 gcgccgggcg cgccgacgtc actgcccctg ccggaggctg cggcggggac ccgctggtca
     1921 agtcggcgag agcttcgtcg aacgtatcac ggtagatcag ccggtttccc atggcgagca
     1981 caactttctt gagctgcggc attcgcgctt ccacggcctg aatgtaaatg gactcgacat
     2041 acaggaaact gttgcccacc ggcagtacga tgatgttacc gcgcagcacc cgcgatccct
     2101 gctggttcca aagcgtgaga tctttggaaa tgttttgatc ctggtccacg cgggattcga
     2161 tttgcatggg accgtaaaca agctgctgct tgggcaattg atagtagatg gcttctccca
     2221 gatgctctcc gtcgcagcgc ccggccatcc agccgatcaa attgtccttt ccccgcggag
     2281 tgaacgggag aatcagcata aattcgggat tcgcctcgcc cggcagggtg gcgacgacgt
     2341 aagtgggcgc catggcctcc ggccggcccg agtgcccgta aaggtcgcgg gcgatctccc
     2401 atacgtcttc cttgttgtag aagacctgtg gatcgcgcat gtggaatatg cggtacgctt
     2461 cggcctgggt gcggaacagg gcctccggat agcgcgtgtg ccgccgcagg tcggcgggca
     2521 tctgggatgc tggccggaac aagccgggga acaggcgttc gtaggcctga atgatcgggt
     2581 cggcggggtc aaacacgtag agcgccatcc gcccgtcata agcatccacc gtggccttca
     2641 ccgagttgcg aatgtaattg gcgccgcccg ccaggcccgt caccggaaga gtcgccgagt
     2701 agggatgcga ccgggaagtt gtgtagccgt cgagcagcca caccaaacga ccctcgtccg
     2761 tgatgaccat gtaggggtcg gcgtcccaaa gcagaaagcc ggccaggtgg gacaggcgcg
     2821 gccggatatt gcggtagatc atcatccggc tctcgccggt caaatatccg gtcagaagaa
     2881 tgttcggctc cccgaaggag atcgccgccg ccgtccgggt gagcagagat cccaccggga
     2941 atccgcccgt gccctgatat cgggagtact tattctggtc gcccgaggga ta
//
LOCUS       DASREK010000089         8781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2031242, whole genome shotgun sequence.
ACCESSION   DASREK010000089 DASREK010000000
VERSION     DASREK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031242"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1218)
                     /locus_tag="VFA54_04655"
     CDS             complement(61..1218)
                     /locus_tag="VFA54_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50128.1"
                     /translation="MSLLFGAGLEAKAQSLDFEFYRTRVEPIFLKKRPGLARCYVCHS
                     ANNTGLRLQPLPRGATSWTEEQSRRNFQSVSQLVVPGDPLSSRLLMHPLAHSAGGDPF
                     HGGGQQFASQNDPDWQTLADWVRRAGTAGSAGQSTTSGESLDFGYYRAHVEPMFLKQR
                     ENGAMCYNCHSVINTRLRLERLSPGRTFWTQEQSRRNFEAVVRLVTPGEPLKSRLLLH
                     PLAPEAGGDPMHTGGKFWKSQADPEWQMLAEWVRSGHAAEAAAPPGPVLDYEFFKTRV
                     EPIFLKKRPGLARCYVCHSANNTGLRLERLPPGATTWTEEQSRRNFQSASQLVVPGDP
                     LSSRLLMHPLAHSAGGDPFHGGGQQFASQNDPDWQVLAEWVRGQKATTSSK"
     gene            1470..2732
                     /locus_tag="VFA54_04660"
     CDS             1470..2732
                     /locus_tag="VFA54_04660"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZS50129.1"
                     /translation="MRDFRFISRPALVLLFSACLAAAALPKASPEDAGLSSERLKRVH
                     ALLERYIDQGEIAGAVSLVARRGRVVHWEAQGLMDLETRQPMRPDAIFRLASMTKPVT
                     SLAVMMLHEEGRFLLDDPVSKFLPEFKSMNVAVANAPNERYPGGYRLVPADRAITIRH
                     LLTHTAGLPSGTSGPTMEAMHKLASLHRPETLLADYIKTLAQLPLNFQPGAAWEYGSA
                     TDVLGRLVEVASGQPLDQFFRQRIFAPLGMNDTWFYLPDDRLPRLASAYAKKNGKLEK
                     LTSPNVDSRNGRFFSGAGGLAGTAEDYFRLCQMFLNGGQFNGVRIVSRKTVEMMTANQ
                     IGRIPLWQDAYRGYGFGLGFRVRERLGESQTLGSTGEYGWGGAYGTYFWVDPKEQMIG
                     ILMIQLNPYAHLNIRPEFQNAVTQAIID"
     gene            2910..3767
                     /locus_tag="VFA54_04665"
     CDS             2910..3767
                     /locus_tag="VFA54_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZS50130.1"
                     /translation="MRLSVGSVLFAPLVLWSGCLPTGLGPAAYGRVEQTVRFDPDGRF
                     RLDNVNGRIEIETWEKQEVAIEAEESASNRRLLDQVHIAIRGEGARVEVHTETPHGGW
                     FLGAGSTKVDYRVRIHKQARVEVKTVNGLVRVDGVAGSVRAETVNGAVRIADASGSVE
                     AKTVNGSIQAEYHDPGDGRHAFSTVNGAVTLYLPGGARGYINARTVNGRITADFPLQT
                     SSKPGRRRLEGRLGEGGGIFEIHTINGAVRVLKSADKIVQRTLLQSCRLPVSACVWTL
                     KSAPIRNLS"
     gene            3728..3985
                     /locus_tag="VFA54_04670"
     CDS             3728..3985
                     /locus_tag="VFA54_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50131.1"
                     /translation="MDPQERADQEFELIYIAAGVGEAQSIEAILDHEGIDYAIQVEQY
                     RSGVIFASVRAGAFFYVPPEFAARSRETLTRLGYRVQTPIE"
     gene            complement(3967..4980)
                     /gene="hemH"
                     /locus_tag="VFA54_04675"
     CDS             complement(3967..4980)
                     /gene="hemH"
                     /locus_tag="VFA54_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZS50132.1"
                     /translation="MSDRRIGVVLFQLGGPDSLEAVEPFLYNLFSDPDIIDFPLARIA
                     RQPLARLIASNRAKKVRSHYMEIGGRSPIRELTERQAEALAAALRPAYNAKVVVAMRY
                     WRPLTQDAIEQLRSEPVSDLVLLPLYPQYSSTTTGSSLREWNRRAAEAGMNGTPTHLI
                     REFYDHPGYLDSLVEQINKALAAFPQMRDVHLVFSAHNVPTSVVQAGDPYPSQIEATV
                     RLVAERGGWGLPAHLCYQSKVGGARWLEPSLDQTIEHLAARGVKNLLVIPIAFVSDHV
                     ETLSEINIEAREHAARCGIAQFEMMPGLNDSPRFIQALADLVRQVVERTAAADEKCYS
                     IGV"
     gene            5133..5768
                     /locus_tag="VFA54_04680"
     CDS             5133..5768
                     /locus_tag="VFA54_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZS50133.1"
                     /translation="MAESTVQGQQAVTGVNGGSDVKAAGTPPKTKKEDTSGKPGVAEL
                     LLKDQPEPQLISTRRRIVTASVLGFLTVNFLMFLRFFFPRTLFEPKTKFRIGYPADFG
                     YGVDTKFQQQYRIWVVRNAEGLFVIYARCTHLGCTPDWKPSENKFKCPCHGSGYDSEG
                     INFEGPAPRPMDRAHVELDPQGQIVVDTSILYSWPKGERSHFNDPGAILKV"
     gene            5825..6769
                     /locus_tag="VFA54_04685"
     CDS             5825..6769
                     /locus_tag="VFA54_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HZS50134.1"
                     /translation="MPENEGNGSGKKAAAPPAAAGGGNGKKGLAALLDREQIEALKEK
                     IKEDAKALKQPTKTDLYKSIFRVKHDETPRSRALGVLTNVFLHLHPAKINRDAVAYNY
                     TWGMGGITFYLFIVLTFTGTLLMFYYHPSKVVAFRDVMFLEHDVPFGKLLRNMHRWAA
                     HLMIITVWLHMFRVFLTGSYKKPREFNWVVGVILLVLTMLLSFTGYLLPDDQLGFWAV
                     TVGTNMARATPLLGHEGPFGPQLGMTPFNDVRFALLGGSIVDSNALLRAYIWHCIAIP
                     IIASTFMVVHFWRVRKDGGISGPAPVVLESEIKPERKL"
     gene            6845..7780
                     /locus_tag="VFA54_04690"
     CDS             6845..7780
                     /locus_tag="VFA54_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HZS50135.1"
                     /translation="MDWSQIWEVATAPDNVPITALLGLVPFYTWYGLRQAKANDRLIE
                     QLETNPEMAKTHHRKVQPWKPGWAREVQVWPYLLRIEFLAALIVTIILMVWSITLNAP
                     LEEPSNPNLTMNPAKAPWYFLGLQEMLVYFDPWMAGVVLPSLIIVGLMAIPYIDANPL
                     GAGYYTFKQRKWAILTFCFGFLGLWVLMVVIGTFIRGPGWMWFWPGMTWDHDRLIFEV
                     NRDLPDLFGITSFLGKGVLGAAAVGLYVALTGYGFHKLITRPGFNRKIYERMNIFQYL
                     TMQALLILMMSLPVKIALRLLFRIKYVWITPWFNI"
     gene            7790..>8781
                     /locus_tag="VFA54_04695"
     CDS             7790..>8781
                     /locus_tag="VFA54_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50136.1"
                     /translation="MPPSAPTPDPDPVTSKSLSRLLLVSSLILVMTLVWALLDETYFL
                     RPWKRYQRQFVQLYSSYLKKLQPVQGQSEDAIRKSATYQQLAKQIDDARKAAAPELDR
                     INKELTTVIQPRLSDVTPEFQTVRSHIAALTYDLEHAASDSARKSIQSEIDEVRKEKH
                     SLTVTKTDGSGTSERVQWDFAQMESEFNRLRDRRAELQTQIIKINAPRTELEAKLAAY
                     VKDQMTGLTREQIQGLLQNMENFKVEIKQIHVKEIDLVDRCESCHLGTREPVQLTKAS
                     MGGQEVFVSHPEPDLLKIHDPERFGCTPCHNGNGIATTSVEKAHGNYEHWLWPL"
BASE COUNT         1764 a         2549 c         2690 g         1778 t
ORIGIN      
        1 cggagcggcc ctgatccgcc ggccgcggca atcccggcgg tttcatctcg cacaagcctg
       61 ctatttggat gaagtggtgg ctttttgacc ccggacccac tctgccagga cctgccagtc
      121 tggatcgttc tgcgaggcaa actgctggcc gccgccatgg aaagggtctc cgccggccga
      181 gtgcgccagc ggatgcatta gcagccggct ggaaagcggg tcgccaggaa ccaccaactg
      241 cgaggcgctc tggaaattgc ggcgggactg ctcctcagtc caggtcgtag ccccgggggg
      301 gagccgctcg aggcggaggc cggtattgtt cgccgagtgg cacacgtaac agcgagccaa
      361 cccggggcgc ttcttgagga aaatcggttc gacacgggtc ttgaaaaact cgtagtcgag
      421 cacgggcccc ggcggcgccg ccgcttccgc ggcgtggccg ctgcgcaccc attcggctag
      481 catctgccac tctggatcgg cttgcgattt ccagaatttt cctcccgtat gcatcgggtc
      541 gcccccggcc tcgggcgcga gggggtgcag cagcagcctg ctttttagag gctcgcccgg
      601 cgtcaccagc cgtacgacag cctcaaagtt gcggcgcgat tgttcctggg tccagaaggt
      661 gcgtcccggt gaaagacgct ccaaacggag ccgcgtgttg atcactgagt ggcagttgta
      721 gcacatcgcg ccgttttcgc gctgcttcaa gaacatgggc tcgacgtggg cccggtaata
      781 tccgaaatcg agcgactccc cggaggtcgt ggactggccc gcggagccgg ctgttccggc
      841 ccgccggacc caatcggcca gtgtctgcca gtccgggtca ttttgtgagg caaattgctg
      901 gccgccgcca tgaaaagggt ctccgccggc cgagtgcgcc agcggatgca tcaacagccg
      961 gctcgagagc gggtcgccgg gcacaaccag ttgggaaacg ctttggaaat tgcggcgcga
     1021 ttgctcttcg gtccaggaag tagcgcctcg cggcagcggc tgaagccgca aaccggtgtt
     1081 gttggccgaa tggcaaacgt agcagcgggc tagcccggga cgctttttca gaaaaatcgg
     1141 ctcgacccgc gttctgtaaa actcgaagtc caaggactgt gccttcgcct cgaggccggc
     1201 gccaaacaac agcgacacag ccagcaggaa aaccggaaag gtacgcccgg tgcaactccc
     1261 gcggtatagc gcaattttct gagagttgag cggtcgcacg gtcaatgcct ccagtttgcg
     1321 gcgttagttt agcacaggcg tatgcgaggc aaaagggctc cgcccggcag gggaagagct
     1381 tcagcaggca gcctcgtcac ggtggaaaaa gggcgccgct ctgttccttg ctactcggtg
     1441 tatactacgc caatcccgga ggctgaaaaa tgagagattt ccgattcata tcccgccccg
     1501 cgctggttct gcttttcagc gcgtgcttgg ctgccgccgc tctcccgaaa gcgagcccgg
     1561 aggatgccgg gctttccagc gagcgtctca aacgcgtcca cgcgctgttg gaacgctaca
     1621 ttgaccaagg cgagatcgcc ggcgccgttt cgttggtcgc ccggcgcggc cgcgttgtgc
     1681 attgggaggc ccaaggtttg atggacctcg aaactcgcca gccgatgcga ccggacgcca
     1741 ttttccggct ggcatccatg accaagcccg tcaccagcct ggcggtcatg atgttgcatg
     1801 aggagggacg ctttctgctg gacgaccccg tctccaaatt tctgcctgag ttcaaaagca
     1861 tgaacgtcgc tgtggctaat gcccccaacg agcgctaccc gggagggtac cgcctggttc
     1921 ctgccgaccg tgccattacg atccgtcatt tgctgacgca cacggccggc ctgcccagcg
     1981 ggaccagcgg gcccactatg gaagccatgc acaagctggc atccctgcac aggcccgaaa
     2041 ccctgctcgc cgattacatc aagaccttgg ctcaattgcc cctcaatttt cagcctggcg
     2101 ccgcctggga atacggatca gccaccgatg ttttgggccg gctggtggaa gtagcttccg
     2161 gccagcctct ggatcagttc tttcgccagc gaatttttgc tcccctggga atgaatgaca
     2221 cgtggtttta tctccctgat gaccggctgc cgcggctggc ctcggcctat gccaaaaaga
     2281 atggaaagct ggagaagctg acctcgccca acgtggatag tagaaacggc cgctttttta
     2341 gcggcgcggg aggtctggcc ggcacggcgg aggattattt tcgcctgtgc cagatgttcc
     2401 tcaacggcgg ccagttcaac ggcgtccgga tcgtcagccg caagaccgtc gagatgatga
     2461 ctgccaatca gattggccga atcccgctct ggcaggacgc ctatcgaggg tacggcttcg
     2521 gcctcggctt ccgcgtccgg gagcgtctgg gcgaatcgca aaccctgggc tccaccggcg
     2581 aatatggctg gggcggcgcc tacggaacgt atttctgggt ggatccgaaa gagcagatga
     2641 tcggcattct gatgatccag ttgaatccgt atgcgcatct caatattcgg cctgagttcc
     2701 agaatgcggt gacccaggcg atcattgatt agctgtccgg ggagtaagcg atccgggctg
     2761 ttgagcatca gttccgggtt tagctgcaag aacagcctgc cagtggcctt aagctgccaa
     2821 ttggcggggg tcatcactgc ttttttctgg gcgcggggaa cttctgacct ctctttctcg
     2881 taaagcaaga gaaaaccgga gaaaattaca tgcgtctctc ggtgggatcg gtcttatttg
     2941 cgcccctggt actgtggtcc ggctgcttgc cgaccgggtt gggccccgcg gcctatggcc
     3001 gcgtcgaaca gaccgttcgt ttcgatccag atggccgttt tcggctcgac aatgtgaacg
     3061 gaaggattga gatcgaaacc tgggaaaaac aggaagtcgc aatcgaagcc gaggagtcgg
     3121 cttccaaccg gaggctcttg gaccaagtcc acattgcaat ccggggcgag ggagcccgag
     3181 tcgaggtgca tactgagacg ccgcatggag gatggttcct gggcgccggc agcacaaagg
     3241 tagattatcg agttcggatt cacaagcagg cgcgcgtcga ggtgaaaacg gtaaacggct
     3301 tggtgcgcgt ggacggagtc gctggttcgg tgcgggccga gactgtcaat ggcgccgttc
     3361 gcattgcgga tgcctcggga tcggtggaag caaagacggt gaatgggagc attcaggcgg
     3421 aatatcacga cccgggtgac ggcaggcacg ctttctccac tgtgaacggc gccgtgaccc
     3481 tgtatttgcc tggcggcgct cgcggctaca tcaatgcccg tacagtaaac ggccgcatta
     3541 cggccgattt tccgctgcaa accagcagca agccgggccg gcgccgcttg gaaggccgtc
     3601 tcggcgaagg gggcggcatt tttgaaatcc acacgatcaa cggcgctgtc agggtgctca
     3661 agagcgccga taaaatcgtt cagcggacct tgctacaatc atgtcgtttg ccggtttctg
     3721 catgcgtgtg gaccctcaag agcgcgccga tcaggaattt gagctgattt atatcgccgc
     3781 cggagtcggc gaggctcaaa gcatcgaggc gattctcgac cacgagggaa tcgactacgc
     3841 gattcaggtg gagcaatacc gctccggtgt aattttcgcc tcggtgaggg ccggagcctt
     3901 cttttacgta ccgccagaat tcgcggcgcg ttcccgtgaa acgcttacac ggctcggata
     3961 ccgcgttcag acgccgatcg agtaacactt ctcatcggcc gccgcggtgc gctcgacaac
     4021 ctgcctcacc agatccgcaa gagcctgaat gaagcgcggg gagtcgttca agccgggcat
     4081 catctcgaac tgcgcgatgc cgcagcgcgc ggcgtgctcc cgggcttcga tattgatctc
     4141 gctgagcgtt tccacgtgat ccgagacaaa ggcaatggga atcaccagga ggttcttcac
     4201 gccgcgcgcg gccagatgtt cgatcgtctg atcgagcgac ggctcgagcc accgggcgcc
     4261 gccgactttg ctctggtaac acagatgcgc cggcaacccc caacctcctc tttccgcgac
     4321 cagcctgacc gtagcctcaa tttgggacgg gtatgggtcg cccgcctgca ccacggatgt
     4381 cggcacgttg tgcgcgctga agaccagatg aacgtcgcgc atttgcggaa aggcggcaag
     4441 agctttgttg atttgctcga ccagggagtc gaggtaaccg gggtggtcgt aaaattcgcg
     4501 aatcagatgc gtgggcgttc cgttcatgcc ggcctcggcg gccctgcggt tccattcgcg
     4561 caggctgctc ccggtggtcg tcgaggagta ctgcggatac aggggaagta atacgaggtc
     4621 gctgaccggc tcggatcgca actgctcgat ggcgtcctgg gtcagcgggc gccaatagcg
     4681 catggcgacg acgactttag cgttgtaagc cgggcgcagc gcggcggcga gcgcttccgc
     4741 ttggcgttcg gttagctccc ggatcggcga gcggcccccg atttccatgt agtggctgcg
     4801 gacctttttt gcgcgattgc ttgcgatcag gcgcgccagc ggctggcggg cgatgcgggc
     4861 cagcgggaaa tcgatgatat ccgggtctga aaaaaggtta tacaaaaaag gctcgacggc
     4921 ttccagcgag tccggcccgc ccagttgaaa caaaaccacg ccgatgcgtc tgtctgacaa
     4981 aagttccctc tcccgttttc ggtgatttcc gaatctttca ttgtagagaa tcgcacctgg
     5041 tgcgggccac ccccgccctt gacaggctca tgcgcggtta ataacaataa ggtttaacca
     5101 gccctacggt tttaaccagc caaggagacg taatggcaga atctacagtg cagggccagc
     5161 aggccgttac gggtgtgaac ggcggctctg acgtgaaagc ggcaggcaca ccaccgaaga
     5221 ccaagaaaga agacacttcc ggcaagccgg gcgtcgccga acttcttttg aaagatcaac
     5281 ccgaaccgca gctcatcagc acccgccgcc gaatcgttac ggcctccgta ttgggttttc
     5341 ttacggtcaa ttttttgatg tttctgcggt tttttttccc gcgaaccctg ttcgagccca
     5401 agactaaatt tcgaatcggg tatcccgccg atttcggcta cggcgtggac acgaaattcc
     5461 agcagcagta ccggatttgg gtggtgcgaa acgccgaggg gctgtttgtg atttatgcgc
     5521 gctgcaccca tttgggctgc acgcccgact ggaaaccgag cgagaacaag ttcaagtgcc
     5581 cgtgccacgg aagcggctac gacagcgagg gcatcaactt cgaaggtccg gcgcccaggc
     5641 caatggatcg cgcacacgtc gaacttgacc cgcagggtca gattgtggtg gataccagta
     5701 ttctctatag ctggcccaag ggcgaacgat cacactttaa tgatcctggg gcaatcctga
     5761 aagtatagct ggatgggcga agagctccgg ctttttcgcc tgccggcgga tctgggttta
     5821 aaaaatgcct gaaaacgaag gtaatggaag cggcaagaag gcggcagccc cgcccgcggc
     5881 tgccggcggc ggcaacggga aaaagggtct ggcggcgctg ctcgaccgcg agcaaatcga
     5941 agctctgaag gaaaagataa aggaagacgc caaggcgctc aagcagccta ccaaaaccga
     6001 tctttacaaa tcgattttcc gggtcaagca cgatgaaact ccgcgcagcc gcgcgctcgg
     6061 cgtgctgacc aacgtctttc tacatttgca cccggcgaag atcaatcggg acgcggtggc
     6121 ctacaactat acctggggga tgggaggcat cacgttctac ctcttcatcg tgctgacctt
     6181 cacgggcact cttttgatgt tctactacca tccgtcgaag gtcgtggcct tccgcgatgt
     6241 aatgtttctg gagcacgacg ttccattcgg aaaactgctg cggaacatgc accggtgggc
     6301 cgctcatctg atgatcatca ccgtgtggct gcacatgttc cgggtgtttc tcaccggctc
     6361 ttacaagaag ccgcgggagt tcaactgggt ggtgggggtc atcctgcttg tcctcacgat
     6421 gctgctctct tttaccggct atctgctgcc tgacgatcag ctcggctttt gggcggtcac
     6481 ggtaggcacc aacatggcgc gcgcgacgcc attgctgggc cacgagggac cgttcgggcc
     6541 gcaactgggc atgacgcctt tcaacgacgt gcggttcgct ttgctcggcg ggtccattgt
     6601 cgattccaac gccttgctgc gcgcctatat ctggcattgc attgcaatcc cgattatcgc
     6661 ttccacgttt atggtcgtgc acttctggcg cgtgcgaaaa gacggcggaa tttccgggcc
     6721 ggcgcccgta gtgctcgagt cggaaatcaa gccggagaga aaactttgat cgctgggggt
     6781 gcagccaagg cctggggagg ttggaacagg gccgccgccg ctgcgtatta aaaggggttt
     6841 cacgatggat tggtctcaaa tttgggaagt cgccaccgcg ccggataacg tgccgattac
     6901 ggccttgctg ggactggtgc cgttttacac ctggtatggg ctgcggcagg cgaaggcgaa
     6961 tgaccgcctg atcgaacaac tcgaaacgaa tcccgagatg gccaagaccc accatcgcaa
     7021 agtccagccg tggaagcccg gctgggccag agaggtgcag gtctggccgt atcttctgcg
     7081 tattgagttt ctggccgcct tgatcgtcac tattatcctg atggtctggt cgatcacatt
     7141 gaacgcgccg ctcgaggagc cttccaatcc gaatctcacc atgaacccgg ccaaggcgcc
     7201 gtggtacttc ctgggattgc aggaaatgct ggtttacttc gacccgtgga tggcgggcgt
     7261 ggtgctgccc agtctgatca tcgtggggct gatggccatt ccctatatcg acgcgaatcc
     7321 gctgggcgcc ggatattaca cgttcaagca gcgcaagtgg gccattctaa ctttctgctt
     7381 cggtttcctt ggcctgtggg ttctgatggt cgtcatcggg acgttcatac gcggccccgg
     7441 ctggatgtgg ttctggccgg gcatgacctg ggatcacgac cgcctgattt tcgaggtgaa
     7501 ccgcgacctg cccgacttgt tcggcatcac gagcttcttg ggaaagggag ttttgggggc
     7561 ggcggcggtc ggcctgtacg tggccctcac gggctacggt tttcacaagt tgatcacccg
     7621 tcccggattc aacagaaaga tttacgagcg gatgaacatc ttccagtact tgaccatgca
     7681 ggcgctgctc attctgatga tgagcctgcc ggtaaagatc gcgctgcgat tgctcttccg
     7741 catcaagtac gtatggatta ctccatggtt caacatttga gggttgaaaa tgccgcctag
     7801 cgcacccacc ccggaccccg atccggtaac cagcaaatcg ctcagccgtt tgctgctggt
     7861 ttcttcgctg attctggtga tgacgctggt ctgggctttg ctcgacgaga cttattttct
     7921 gcgcccctgg aagcgctacc agcggcaatt tgtacagctt tattcttcct acttgaagaa
     7981 gctccagccg gtgcaggggc agagcgagga cgcgattcgg aagtcggcaa cctatcagca
     8041 gttggccaag cagatcgacg acgctcgaaa agcggccgct cccgagctgg atcgcatcaa
     8101 caaggagctc acgaccgtta ttcagccccg gttgagcgat gtgacgccgg agtttcaaac
     8161 ggtccgcagc cacattgccg cgctcaccta cgatttggag cacgccgcct ccgacagcgc
     8221 caggaagtcg attcagagcg agattgacga ggtgcgcaag gagaaacatt cgctcaccgt
     8281 gaccaaaacc gacgggagcg ggacctccga gcgcgtgcag tgggatttcg cgcagatgga
     8341 gagcgaattc aaccgcctgc gagaccgccg cgccgaactg caaacccaga ttatcaagat
     8401 caacgccccc cggaccgaat tggaagccaa gctggcggcg tatgtgaagg atcagatgac
     8461 cggcctcacc cgcgaacaga tccagggctt gctccagaac atggagaact tcaaggtcga
     8521 gatcaaacag atccacgtaa aagagatcga cttggttgac cgctgtgaat cctgccacct
     8581 gggcacgcgc gagccggtgc agctcaccaa agcgtccatg ggagggcagg aggttttcgt
     8641 cagccacccc gagcccgacc tgctgaagat tcacgacccc gagcgcttcg gctgcacgcc
     8701 ctgccataac gggaacggca ttgcgaccac gagcgtagag aaggcgcacg gtaattacga
     8761 gcactggttg tggccgcttt a
//
LOCUS       DASREK010000090         5789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2360682, whole genome shotgun sequence.
ACCESSION   DASREK010000090 DASREK010000000
VERSION     DASREK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360682"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFA54_04700"
     CDS             complement(<1..939)
                     /locus_tag="VFA54_04700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS50137.1"
                     /translation="MPLPNCGRNSRSAILGDFLSIGLACGLLALMALLMSWSAAGDAV
                     TSDEDEHISAGFSYLTKGDLRLNPEHPPLMKDLAALPLLGMNLKAPWDHPSWTEEVNG
                     TDRFARAFLLHSGNDLETIVQTARKPMIVFTVLLGLAVFFCTRCMFGGAAALLATFFF
                     AFSPTFLAHGRLVTTDVGASAGFFIATIAYLHFLEKPTIPNVLLAGAGLGFALLTKFS
                     TFLLLPIDFILTLLWAALSRSSPVTAQEKCHTCRAERETHKIHTVRRWLGGYFAIVTL
                     AFLTIFPVYWLHTRNYPPERERRDATEILSWLSVGVM"
     gene            complement(1411..2847)
                     /locus_tag="VFA54_04705"
     CDS             complement(1411..2847)
                     /locus_tag="VFA54_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZS50138.1"
                     /translation="MDRNLSHYRRASTRREFFARAGSGLAGIALAHMLHEDGLLAASA
                     SAVDPLAPKPPHHPPTAKSIIWLFMEGGPSHVDLFDPKPKLKELQGQPIPESMRPKFT
                     AMPGTSKNGLMPSKRTFKQYGQSGIWVSDWYPNVAQHVDDMTVIRSCWTDGINHLGGV
                     TEMNSGSILSGRPCLGAWVNYGLGSANRNLPSFVVMLDDRDPIGGAKQWSAGFLPASY
                     QGTQFRQGDTPLLHLKPPAGMTDSEQRNELALLKRLNEIWSEDKQEDSELDARIRSYE
                     LAYKMQSAAPEAVDLSKESEATKKLYGLDVEETRAFGTNCLLARRLVERGVRFVELYC
                     GSGSGWDAHENVETNHSKWCKASDKPIAGLLTDLKARGMLKDTLVVWGGEFGRTPFNE
                     KGLGRDHNPWGFTIWMAGGGVKGGQYIGSTDEIGMYATERRMHVNDIHATMLWALGLD
                     HLRVTYMHNGRAERPTVVAGEVNKDVFA"
     gene            complement(2865..5486)
                     /locus_tag="VFA54_04710"
     CDS             complement(2865..5486)
                     /locus_tag="VFA54_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZS50139.1"
                     /translation="MLVAVAAVKPQQVLRLFWPAAILIACAAPAQTAKKVSFSHDVVP
                     ILSQKCMQCHGLAQPMANLDLRSREGALKGGQHGPALVPGDAAASHIYQHITGQEQPQ
                     MPLGGRLSDEEIAVLRDWINSGAEWDSNITLSAPSPVAAATAAAPEKKFTEAQRRYWA
                     FQKVVKPSVPAVKNRNWVRTPIDAFILAKLEEKNLRPNPPADKITLIRRAYFDLIGLP
                     PSPEDVQAFLADNSPGAFEKVVDRLLASPQYGERWGRHWLDLARYADTQGFKADETRP
                     NVWRYRDYVIRAFNEDKPYDRFIKEQIAGDELYPDDPMAKIATGFDRLWPDESNLANP
                     ILMRQEILNDITDTVSAVFMGLTYGCAKCHDHKFDPILQKDYYRLQAFFAASRNEDHT
                     RLFTGQQAKAYQEQYAAWDAQTRDIRSKMRQILDSVREDKTKEAIGMFPKEAQDAIFT
                     PPEKRTPMQWQMYYRSESRLPTDAALEKSLKGEARQQYAELKKQLASYDHLKPPEPPL
                     AEVMIDQGPAAPPTFILSKGVWDAPLQEVEPGFLSILDPAPAKIVPPEGVKSTGRRSA
                     LAKWLADPANPLTTRVMANRIWYYHFGHGIVDSPSDFGIMGERPSNPQLLDYLTATFV
                     ENGWSIKKMHRLIMLSNAYQQSSAYQPEAAKVDPNNKLLWRFDRRRLEGEAIRDSMLY
                     VSGELNLKMGGPGVFPPLPPGVSMPGSRYLNWETEKDPAEANRRSVYVFVKRNLRYPM
                     FETFDFPDTHESCPRRYATVTPTQPLALMNDELVMSWSQALAGRVLNDSGLSPAQQVD
                     RAFRLVFSRSPKEEERQAVLEFLDKQAGEIAKQLANNEKVPMPEAIPQGMDPARAAAF
                     VDFCQALLNSNEFIYIN"
BASE COUNT         1159 a         1735 c         1708 g         1187 t
ORIGIN      
        1 catcactcca accgagagcc agctcaggat ctctgtagcg tccctccgtt cccgctcagg
       61 cgggtaattg cgcgtgtgga gccagtaaac gggaaaaatc gtaagaaacg caagagtcac
      121 gatcgcgaaa tatccgccca accaccggcg aacggtgtgg attttgtgcg tttctctttc
      181 agcccggcat gtgtggcatt tctcttgcgc tgtgaccggc gaggagcggc tcagagcggc
      241 ccacagcaac gtcaggatga agtctatcgg caggagtaga aacgtcgaaa actttgtgag
      301 gagggcgaat ccgagtccgg cgccggctag aaggacgttc ggtatggtcg gcttttctaa
      361 aaaatgaagg taggctatcg tagcgatgaa aaagccggcg ctcgctccga catccgtggt
      421 taccagacgt ccgtgggcga gaaaggttgg cgaaaaagcg aagaaaaacg tcgcgagcag
      481 cgcggcggcg ccgccgaaca tacatcgcgt gcaaaagaaa acggcaaggc ccaatagaac
      541 agtaaagacg atcatgggct ttcgggcggt ctgcacaatc gtttcgaggt cgttgccgga
      601 atgcaatagg aaagctcgcg cgaaccgatc ggtcccgttc acttcttcgg tccagcttgg
      661 atgatcccag ggggccttca ggttcattcc tagaagcggc agcgccgcga ggtctttcat
      721 cagcggcgga tgttcggggt tgaggcggag gtcccctttg gtcagatagc tgaaaccggc
      781 gctgatgtgc tcgtcttcgt cgctggtaac agcatcgccg gcggcgctcc aagacatcaa
      841 caaggccatc aaggcaagca agccgcaagc gagcccgata gacagaaaat ccccgagaat
      901 tgcacttcgc gaatttcggc cgcagttggg caacggcatg gttcctgcgc agcctccgcc
      961 cgggcgatat gttatcccaa aacacaccgc agccatcgac ctttcgagat gtttagcgga
     1021 ttcctcgatc ttcagcccgc ctgcggacaa agcgtcaggg gcccggcata gcgtgggacc
     1081 tggtcaaaca tgtagggggc gcaacactga agggcgggcc gaggccccag ccgctcggag
     1141 caatcgaccg tcgcccgaga gctagcacgc cacagcgggc gctggaaact tgagcgcctt
     1201 ttcgcaggaa taccgccagc gccggagccc gcccgacaac ttaccgctac ccgcagccaa
     1261 ccggccgagc ttccgcacta taacgggcga ggacatggat gcgcagcttg tgtgggcgcc
     1321 ggccgggcca ggcgtgggtg tggtaatcgg cctcttgcct actcagcgcc cacgcttacc
     1381 agaaaggcgg aagccagata tagaaaaatc tcaggcgaag acgtctttat tcacttcgcc
     1441 tgccacgacc gtcggccgtt ccgctcggcc gttatgcata tacgtaacgc gcaggtggtc
     1501 aagaccgaga gcccagagca ttgtggcgtg aatatcgttc acatgcatgc ggcgctcggt
     1561 ggcgtacatg ccaatttcat cggtggagcc gatatactgg ccgcctttga cgccgccgcc
     1621 cgccatccag atggtgaagc cccagggatt gtgatcgcgg cccagtccct tttcattgaa
     1681 aggcgtacgg ccaaactctc cgccccaaac gacgagagta tcttttagca tgccgcgagc
     1741 cttgagatcg gtgagcaggc cggcgatcgg cttgtcggaa gctttgcacc acttgctgtg
     1801 gttggtttcg acgttttcgt gcgcatccca gccgctgcct gaaccgcagt acagctcgac
     1861 gaaccggacg ccgcgctcga ccaagcggcg cgccagcagg cagttggtgc cgaaggcgcg
     1921 agtctcctcc acatccagtc cgtacagctt tttggtcgcc tccgactcct tcgagaggtc
     1981 cacggcttcc ggcgccgcgc tctgcatctt gtaagcgagc tcgtaggagc ggatgcgcgc
     2041 atccagttcg gaatcttcct gcttgtcctc ggaccatatt tcattgaggc gtttcaacag
     2101 cgctagttcg ttgcgctgct cggagtcggt catgccggcc gggggcttca agtggaggag
     2161 cggagtatcc ccctggcgaa attgcgtccc ctgataagag gcgggtaaga agcccgcgct
     2221 ccactgcttc gcgccgccga tcggatcgcg atcgtccagc atcacgacaa aggaaggcag
     2281 attgcggttg gcgctcccca gtccatagtt gacccacgcc ccgagacacg gccggccgct
     2341 cagaatcgag ccgctgttca tttccgtcac gccgccgagg tgattgatgc catcagtcca
     2401 gcaggagcgg atgacggtca tgtcatccac gtgttgcgcc acattcgggt accaatcaga
     2461 cacccaaatc ccgctctggc cgtactgctt gaacgtgcgc ttcgaaggca tcagcccgtt
     2521 ctttgaggtg ccgggcattg ctgtgaattt cggccgcatg gactcgggga ttggctgccc
     2581 ttgcagctcc ttgagcttcg gcttgggatc gaacaggtcc acgtgactcg gaccgccctc
     2641 catgaacagc cagatgatcg actttgccgt cggagggtga tgtggaggct taggagcgag
     2701 aggatcgaca gccgaagccg aagcggccag caaaccatcc tcgtgaagca tgtgagccag
     2761 ggcgataccc gcgaggccgc tgccggcccg agcgaaaaac tcacggcgcg tggaagcccg
     2821 cctatagtgc gaaagattcc gatccatctt ggcccccttc cttgctagtt gatatagata
     2881 aattcgttgg agttcagcag cgcctgacaa aaatccacaa acgccgccgc ccgcgccgga
     2941 tccattccct gcggaatggc ttcgggcatg ggcacttttt cattgttagc cagctgtttt
     3001 gcaatctcgc cggcctgttt gtccagaaat tcgagaaccg cctgccgttc ttcttccttg
     3061 ggagatcgtg aaaaaaccag gcgaaacgcc ctgtctacct gctgcgccgg agaaaggccg
     3121 ctatcattca agacgcgccc ggcaagagct tgcgaccagc tcatcaccag ctcatcgttc
     3181 atcaacgcca gcggctgggt cggagtgacg gtagcatagc gccgcgggca gctctcgtgc
     3241 gtatccggaa agtcaaaggt ttcaaacatc gggtaccgaa gattgcgctt cacgaacacg
     3301 taaacgcttc ggcggttcgc ttcagccgga tccttctccg tttcccaatt caggtacctg
     3361 gaccccggca tgctgacgcc cggcggaagc ggcggaaata cgcccggccc gcccatcttc
     3421 aagttcagct cgccgcttac gtagagcatc gaatcgcgga ttgcttcgcc ttccagccgg
     3481 cggcggtcga accgccacag cagcttgtta ttcggatcga ctttcgccgc ctccggctga
     3541 taggcggaag actgctggta agcgttcgaa agcataatca gccggtgcat ctttttgatg
     3601 ctccagccgt tctcaacgaa cgtagccgtc aggtaatcca gcagttgcgg gttgctcggc
     3661 ctctcgccca taatgccgaa atcgctaggc gaatcgacaa tgccgtgccc aaaatggtaa
     3721 taccagatgc gattggccat cacccgcgtc gtcagcggat tggcgggatc ggcaagccat
     3781 ttggcgagcg ccgagcggcg gccggtggat ttgacgcctt cgggcggaac aatcttggcc
     3841 ggcgcagggt cgaggatcga gaggaagcct ggttctactt cttgcaacgg cgcgtcccag
     3901 acgcccttcg aaaggataaa cgtgggcggg gcggccggac cctgatcgat catgacttcc
     3961 gccagcggag gctccggggg tttcaaatga tcgtaagagg ccagttgctt cttcagctcc
     4021 gcgtattgct gcctggcttc tcctttgagg ctcttttcca gcgccgcgtc agtcggcagc
     4081 cggctttcgc tgcggtagta catctgccac tgcatcggcg tgcgcttctc aggaggcgtg
     4141 aaaatagcgt cttgcgcctc cttggggaac atcccgatgg cctctttcgt cttgtcctcg
     4201 cgcaccgaat ccaaaatctg ccgcatcttt gaccgaatgt cccgcgtctg ggcatcccaa
     4261 gcagcgtatt gttcctgata ggccttggcc tgctggccgg tgaacagacg ggtgtggtct
     4321 tcattgcggc tggcggcaaa gaaggcctgc aagcggtaat agtctttttg gaggatcggg
     4381 tcgaacttgt ggtcgtggca tttggcgcac ccgtacgtca gccccataaa cactgcggaa
     4441 acagtgtccg tgatgtcgtt caagatctct tgccgcatca gaatggggtt ggccaggttg
     4501 ctctcgtcgg gccagagcct gtcaaagcca gtagcgatct tcgccatcgg atcatccgga
     4561 tagagctcgt cgccggcgat ctgctccttg atgaaccggt catagggctt gtcttcgttg
     4621 aacgctcgga tcacgtaatc ccggtaccgc cacacgttcg gccgggtttc atccgccttg
     4681 aagccttgcg tatcggcgta tcgggccaag tcgagccagt gccggcccca gcgttcgcca
     4741 tactgcgggg aagccagcag gcggtctact accttttcaa aggcgcccgg agagttgtcg
     4801 gctagaaacg cctgcacgtc ttccggcgac ggagggagcc cgatcaagtc aaagtacgcc
     4861 cggcgaatca acgtgatttt atcagccgga ggattgggtc tgaggttctt ctcctcaagc
     4921 ttcgccagga tgaaggcatc gatgggggta cgaacccagt tgcggttctt aacggccggc
     4981 actgagggct ttaccacctt ctgaaaagcc caatagcggc gctgcgcctc agtgaacttc
     5041 ttttcggggg ccgcagcggt cgcggccgca accggcgagg gagccgagag ggtgatattt
     5101 gagtcccact cggcgccgct gttaatccaa tccctcagca ccgcgatctc ttcgtcgctg
     5161 agcctgccgc caagcggcat ctgtggctgc tcctgacccg taatgtgctg ataaatgtgg
     5221 ctggccgcag cgtcgcccgg cactagcgcg ggaccgtgct ggccgccttt gagcgctccc
     5281 tcgcggcttc ggaggtcgag attggccatc ggctgcgcca acccgtggca ttgcatgcat
     5341 ttctgcgaca gtatcggcac tacatcatgg gaaaaagaaa ccttcttagc tgtttgggcc
     5401 ggcgcagcac acgcgattaa aatcgctgcg ggccaaaaaa gacgaagaac ctgctgaggt
     5461 ttcaccgctg ctaccgcaac cagcatttca gcctccgggc actgaggtac ttacgttgat
     5521 tttacccccg attctacaga cgcctcaact aaatttttac ggcgctttta caacgccaag
     5581 tccctggaat ttcctgcgct tcggcgttta gctcacccgc caaatcgcct agcctcgcgg
     5641 gcgccgccgc gccggtgtcc tttggatcgc cagcgggggg ttatggcctc gcggcaaaaa
     5701 cgcagggatc ttcggccacc cccccgcatg cttcggaacg tgggatgttt cgggacatcc
     5761 ttttgaaccg gaaaagctgg ctttgaact
//
LOCUS       DASREK010000091         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1868170, whole genome shotgun sequence.
ACCESSION   DASREK010000091 DASREK010000000
VERSION     DASREK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868170"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFA54_04715"
     CDS             complement(<1..765)
                     /locus_tag="VFA54_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50140.1"
                     /translation="MILKWKQSVLVCVAAGLTALAPPAASGADAAAADPAAMDPAPPP
                     QDQGQASQPAFKLTPYGRVELDIIGSSRGTNPLDPRQFNGYSTAAGPEPHSSSTFNPR
                     FSVLGMQADVNQANQQVQAKVEVDFYSTDSANLITPRLRLAYVQYTRQQTKITAGMDW
                     NPVVPLLPDIVDFSIMGYGGNLWQRLPQVTIRQGIGKNWEVMGTVMRFERGFTLDSKQ
                     FGGADPFNDPIKMPYLGARVAYQKWGAGGNGMVAVSG"
     gene            complement(969..1763)
                     /locus_tag="VFA54_04720"
     CDS             complement(969..1763)
                     /locus_tag="VFA54_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HZS50141.1"
                     /translation="MKSAGLTGELVAEFLGTMVLILFGTGVVAMVALFGTGVRGEIVN
                     GGYTNITLGWGLAVAMGVYVAGGISGAHLNPAVTLALAVFRHFSWRKVLPYSLAQTAG
                     AFAGAAIVYWDYRLAFHRADPWLDHTAGVFTTFPAFPEVFSAGLFDQILGTALLVGLI
                     FAVVDPRNPVAAPNVSPPNLGPLIVGLIVVAIGMAFGGMHGYAINPARDFGPRLLTVV
                     AGFKNNGLTSGRPVFWVPIVGPLLGGLIGGLVYDQGVRRFLPALTK"
     gene            complement(1760..2743)
                     /locus_tag="VFA54_04725"
     CDS             complement(1760..2743)
                     /locus_tag="VFA54_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS50142.1"
                     /translation="MTGLTAAPPKARLSVCFNLAVGPLLGLAAVFAFFAVWAGSPFYS
                     AYNLVTMLTQSVIVASGALGMVIVMIAGGIDLSVGSIIALSTVVIARLLQAGASPWCA
                     ALAGVAVGGFCGALNGLLVTGLGLVPFIVTLGTLLLFRGAATGLAGEQKIDAPITWLN
                     DMLTKFPSPRWMLVASGVWLTLLLSVTVALLLDRTVLGRYAFAIGSNEQTARLCGVRV
                     ERIKIYVYTLGGLLTGFAGLMQFSRLTVGDPTAAKGMELDIIAAVVIGGGSLSGGQGS
                     VLGSLAGALLMTVIRSGLSMKGVPNWVQDVLTGCIIVAAVALDRLRRRGTA"
     gene            complement(2740..>3528)
                     /locus_tag="VFA54_04730"
     CDS             complement(2740..>3528)
                     /locus_tag="VFA54_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS50143.1"
                     /translation="VPHQLGRPVLELDALAAPGVASASLTLHRGEILGIAGLIGAGRT
                     EMLRAIFGLAPVRSGKVTVLAVSSLAPPSSFGRPGPAGGQTVAPATRLAEGIGFLSED
                     RKKEGLAQIASLADNLTLSNLRPYARFGWLNRQAQLRAAAGWISRLAIKAPSAAAPVG
                     SLSGGNQQKVALARLLHSNCDILLLDEPTRGIDVASKAQIYELIGKLAQAGKAILFVS
                     SYLPELLGVCDNIAVMCRGRLGEKAPASAWTERSAMSAAMGEPV"
BASE COUNT          774 a         1090 c         1101 g          563 t
ORIGIN      
        1 gccggaaacg gcaaccattc cgtttcctcc cgcaccccat ttctgatagg cgacccgggc
       61 gccgaggtag ggcatcttga tggggtcgtt gaacgggtcg gcgccgccaa actgcttgga
      121 atcgagcgtg aagccgcgct caaaacgcat caccgtcccc atgacttccc agtttttgcc
      181 gatgccctgc cggatggtga cctgcggcag gcgctgccaa agattgccgc cgtaacccat
      241 gatggaaaaa tcgacgatgt cgggcagcaa aggaacaacc ggattccaat ccatgccggc
      301 tgtaattttc gtttgctgcc gggtgtactg gacatatgcg aggcgcaggc gcggagtgat
      361 gaggttggcg gagtcggtgc tgtagaaatc gacttccact ttggcctgca cctgctgatt
      421 cgcctgattt acgtcggcct gcatgcccag cacgctgaag cgcgggttga acgtggagga
      481 cgaatggggc tccggaccgg cggcggtcga gtagccgttg aactggcgcg ggtccagcgg
      541 attggttccc cggctggagc cgattatgtc cagctcgacg cgcccatagg gagtcagctt
      601 aaatgcaggt tgtgaggctt gcccctggtc ttgcgggggc ggagcggggt ccatagcggc
      661 cggatcggcg gcggcggcat ctgcgccgct agctgccggc ggcgcgagcg cagtcagccc
      721 tgcggctacg cacactaaca cactctgctt ccacttaaga atcattcagc atccacctct
      781 ggaggaattg actgcgactc gaagtgtcag tctacggtga gcgtgcgatt ctgtccaatc
      841 gataatttca attggcggcc aagatttttt tgattcggaa acgcgccgcc taattgatga
      901 aaattcttat ggcgactatt tcgttaaaga acaaacagcg ggcggaaata cagcaaatca
      961 gcgcgcgatc actttgtcaa ggcaggcagg aaacgccgca cgccctggtc gtacaccagt
     1021 cccccgatca atccgccgag aagggggccg acgatgggca cccagaatac gggacgtccg
     1081 ctggtaaggc cgttattttt gaatccggcg acaacggtca gcaggcgcgg gccgaaatcg
     1141 cgcgccggat tgatcgcgta gccgtgcatg ccgccgaagg ccataccgat ggccacgaca
     1201 atcaaaccga cgatgagcgg cccaaggttc ggcggggaaa cgttcggcgc agcgacaggg
     1261 ttccggggat ccaccacggc gaaaatgaga ccgaccagca aagcggtgcc aagaatctga
     1321 tcaaacaggc cggccgaaaa gacctccgga aacgccggaa aagtggtgaa cactcccgcc
     1381 gtgtggtcca gccagggatc ggcgcggtgg aaagccaggc ggtagtccca atacacaatg
     1441 gcggctccgg cgaaagctcc cgctgtttgg gcaaggctgt agggcagaac cttgcgccag
     1501 gagaaatgcc ggaaaacggc gagcgccaga gtgacagccg ggttcagatg ggctcccgaa
     1561 atacctcccg ccacatacac gcccatagct acagccagtc cccagcccag agtgatattc
     1621 gtgtagccgc cgttgacaat ctcgccacgg acgccggtgc cgaacagcgc gaccatggcc
     1681 acgacgccgg tgccgaagag aatcaagacc attgtgccga gaaactcggc gaccaattcg
     1741 ccggtcaagc cagccgattt catgccgtac ctctccgccg cagccggtcc agggctacgg
     1801 ccgccacgat aatgcatccg gtcaacacat cctgaaccca gttaggcacg cctttcatgc
     1861 tgagcccgct gcggatgacg gtcatcaaca gcgcgccggc cagagatccc aggacgcttc
     1921 cctgcccgcc ggaaaggctg ccgcctccga tcacaacagc cgcaataatg tccagttcca
     1981 ttcctttggc ggcggtgggg tcgccgactg taagccggct gaattgcatc aggccggcga
     2041 aaccagtgag caatccgccg agcgtgtaga cgtaaatctt gattcgctca acccgcacgc
     2101 cgcacaggcg cgcggtctgc tcgttggatc caatggcgaa agcatagcgg ccgaggacag
     2161 tgcggtccaa cagcagcgcc accgtcacag agagcaggag cgtcagccac acgccggaag
     2221 ccacgagcat ccagcgaggc gacgggaact tggtcagcat gtcattgagc caggtaatgg
     2281 gggcgtcgat tttctgctcg cccgccaggc cggtcgccgc accgcgaaag agcagcagag
     2341 ttcctagggt cacaatgaaa ggcacgagtc caaggcccgt gacgagcagc ccgttcaggg
     2401 cgccgcaaaa gccgccaacg gccacgccag ccagagctgc gcaccaggga ctcgctccgg
     2461 cttgcaaaag acgggcgatc accaccgtgg agagcgcgat gatcgatcca acgctcaaat
     2521 caatgccgcc ggcgatcatc acgatcacca tgccgagcgc gcccgacgcg acgatcacgc
     2581 tctgcgtgag catcgtgacc aggttgtagg cggaataaaa gggcgaaccg gcccagaccg
     2641 cgaagaacgc gaagacagcg gcgaggccga ggagcgggcc cacggccaga ttgaagcaga
     2701 ccgaaaggcg ggccttcggc ggcgcggcag tcagaccggt cacaccggct ctcccatcgc
     2761 cgcgctcatg gcgcttcgtt cggtccaggc gcttgcgggc gctttttccc caagccggcc
     2821 gcggcacatg acggcgatgt tgtcgcagac gcccagcagc tccggcagat aggaactgac
     2881 aaacagaatc gctttgccgg cctgagcgag tttcccgatc agctcatata tctgagcctt
     2941 gctcgcgaca tcgatgcccc gcgtgggctc atccagcagc aatatatcgc aattgctatg
     3001 gagcagccgg gccagggcga ccttttgctg attgcccccg gaaagagatc cgaccggggc
     3061 agcggcggag ggcgctttga tagccagccg ggaaatccag ccggctgccg cgcgcaattg
     3121 ggcctgccga ttcagccacc cgaaacgggc ataaggccga aggttggaaa gagtcagatt
     3181 gtcagccagg gacgcgattt gcgccaaacc ctccttcttg cgatcttcgc tcaagaaccc
     3241 gattccttca gccagacgtg tggcaggagc gactgtctgg ccgcccgcag ggccgggccg
     3301 gccgaaggaa gaaggcggcg caagcgaaga gacagccagg acggtgacct ttccgctgcg
     3361 cacgggggcg agaccaaaga tcgcgcgcag catctcggtg cggccggcgc caatcagacc
     3421 cgcgataccc aaaatttcgc ctcgatgcaa tgtcaagctg gcggaggcca cgccgggcgc
     3481 cgcgagggca tcgagctcga gaacaggccg gcccaactgg tggggaac
//
LOCUS       DASREK010000092        13676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1536452, whole genome shotgun sequence.
ACCESSION   DASREK010000092 DASREK010000000
VERSION     DASREK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13676
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536452"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFA54_04735"
     CDS             complement(<1..823)
                     /locus_tag="VFA54_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZS50144.1"
                     /translation="MKISLGSWAFSFGPYESHPIPFDRAARRLAEAGYDGVEVCGFPP
                     HVSLNRYPTAAPRRELRVFLQDLGLECSGYAADLTSVNPAAPGNKQKYLDLFQRNLEL
                     CRDLRIPAIRVDSGAAPDSIPDAEYLAARDRLVETWRDAADLGQAAGVRVVWEFEPAF
                     AFNKPSEIRAIADAVNHSNFGILFDTSHAYMSAVMGARGQGTLEVLPGGIPEMLRLLD
                     SRIRHIHLIDSDGSLHNNETSTHVPFGAGKIDFASLAPQLLKIPDIEWWCIDMCFW"
     gene            1087..2151
                     /locus_tag="VFA54_04740"
     CDS             1087..2151
                     /locus_tag="VFA54_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50145.1"
                     /translation="MTQNVVQRFSAPTQAAAILILLLHSGACGPAWLAKQAEFAVENC
                     TALDGAPELSSWRWKVDPDPVLTRGPKGAWDSFDVLNPSVVFWNGLYYNLYSGFDGHT
                     WRTGLATSVDGRHWEKFAGNPVLSPLPGSWEGDYIAANGSALHDGSQFLYWYHAGRPA
                     RIGLARSTDARHWQRLGPPVLEPGPEGSWDESGVADPYVLRCRDAYYLYYLGQNRRAV
                     QRIGAARSPDGIHWQKFIGNPVLDPGPPGAFDERGVGEPAVFRAATEFYMIYTGRSAG
                     ETRRLGAARSSDGVHWQRAALAATIASGQPWDSHVVCDPSVLAVNGKLWLWFGGGDVA
                     RPDERLHGQIGLATASLNGR"
     gene            2258..4246
                     /locus_tag="VFA54_04745"
     CDS             2258..4246
                     /locus_tag="VFA54_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZS50146.1"
                     /translation="MKRFIYLCLLGPWLLLGQSGVPNLKPDPVQWSLRLEPDSAAPGQ
                     KILGRLIATIEPGWHLYSLTTPRPPIATTVAVQPSPAIEKWTVFAPPPKRALDPNFNV
                     ETETYEKNVDFVIDLDLAPSAAPGAAPITAQVRYQACNDKTCLFPARKTAAATLKIDP
                     NIRAATPVIPAGYTEFKGGPPAAGPGTATPTGGGASGGSPVSPAPAAFAGSQGTGQFI
                     ALALGLGFLAIFTPCVFPMIPITMSYFLGAQTGSRRQSVIQAFTFCFGVVLLFTGLGA
                     AVTAILGPFGITQLGSSPWVNLFIALVFLAFGLSLLGAFEITVPSGVLTTVTNASYRG
                     GLAGALIMGLAFSLASFACTGPFVGTLLAGSTQGGLSWPILGMFFFSIGLASPFFFLA
                     LFPAYLKRLPKSGGWLERTKVTLGFLILAAMMKYLSNIDQVYQWNILTRERFLAVWIV
                     MLMLAGLYLLGILKLKEEKDEPVSLGRLFAAAAMLVLAISLVPGMFGARLGELDAYVP
                     APGESALTSGGATAELKWIKDDYRAALDLARQSGKPLFVSFTGYACTNCHWMKANMFT
                     RPEIAAVLKNFVLVELYTDGTDAASEANQKMAETRFNTTAIPFYAVIRPDDTVSGTFA
                     GVTRNAKEFEAFLNRGLGGAATPSRPSAAAVPAATPAS"
     gene            4360..6108
                     /locus_tag="VFA54_04750"
     CDS             4360..6108
                     /locus_tag="VFA54_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZS50147.1"
                     /translation="MIPPLDPGGALAAALSAAVFIVAIVYVVRSGHADIQTPLMSALA
                     GEDALLGRELFSNAILERRLAGRGLLLSVALHAVVLLGTPLLPYLFPDRLNFDFRKYN
                     VKIIEFRIPEPLLYTVPDAKQSRSRERAEPPGRVARGRRNLLARASPAPGSRLPGVAG
                     GPPRLQLPSSAQSRARDVVIEPDQPPQVSIAAPHPLPTAFLWAQGPAPDRLSRLVGEP
                     PAVKLPLFALPHSTPAVQRPNREVALAELQIASGPVLTERPPQLPVLPANISPVRMPS
                     PPIDAPGQLPASALPGSSPLNLIALMQTPALSAPAYLLQTGNRLAEAQPGPNGPSQGA
                     SGGNGSPAANANASETAKDAPKAPGAETSANTRSDTSGAETAPSKGTYADLLAASSPG
                     STGKGSLAADSNAGQAGEQERRKPSGQSYPSGNLGVIIVQQSAQDTILEGSEVLTGQP
                     VYTVYFDVPGAPRRWILQYCVPGSDSKTFFEQPDGVIRILPKRSVQPPFPIERIPLDV
                     KSVPNPGTPVSARRLVIYATVNERGETGNVRLIRGTGQEIDRTAMATLQRWTFRPAMR
                     GDTPVAVEALFGIPLP"
     gene            complement(6129..6356)
                     /locus_tag="VFA54_04755"
     CDS             complement(6129..6356)
                     /locus_tag="VFA54_04755"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HZS50148.1"
                     /translation="MRVTGAALAGINAAIRQIDQAADNLSRLADPSSAAEIDVSKEAV
                     RLLSARSAFTANVKLIRAANELDEKLLDLLA"
     gene            6427..7431
                     /locus_tag="VFA54_04760"
     CDS             6427..7431
                     /locus_tag="VFA54_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZS50149.1"
                     /translation="MEHKVLITVSPIYSEAVNFLTARLKGAGFDVQYLNQPDEQLSAA
                     ELSDLLRGVEVYVVGNARVPRQAIQSSPRLALVAKYGVGVDNIDLAAATDHGVLVTNA
                     PGENAISVAEMTIAGLLNLSRKLREIERSFRQGKWRAVFGHEIYQRTLGIIGLGNIGK
                     QVAIRARAFGMRILAYDLLDYPEFCREHQVEMTPLDRLLAESDAVTLHVPLTPETRHM
                     IGAPQFSRMRPGALLIHTARGGVVDEQALYLALADGPLGGAAIDVFECEPLGESPLKR
                     LDNVILTPHVAGISYEAAERVAARTLENVLAYQAGRTPPCLLNPEAARLRKKLDFVDR
                     "
     gene            7568..8029
                     /locus_tag="VFA54_04765"
     CDS             7568..8029
                     /locus_tag="VFA54_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50150.1"
                     /translation="MHLSDRRRAPWLILVVDDDYGDQILIQEALQASGICKQIQIVSD
                     GEEAMEYLLGQGRYAAPGSCRRPDLILLDLNMPRLGGKEVLALVKADQNLRSIPVVAF
                     TTSGRQEDISQCYSLGVNSYVQKPVNFQEFQCVLRRVEEYWLEISLPPPPG"
     gene            8043..8456
                     /locus_tag="VFA54_04770"
     CDS             8043..8456
                     /locus_tag="VFA54_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50151.1"
                     /translation="MQRALTILHIDDDSDTRFLLRELISESPEPEGGEHTEICWLDAS
                     GVEEAVAQFAGAPLDAILLDNRLAGENGVELLPRIRQVWDCKVWILTGFFHEKLHDES
                     LQRGAAGVIGKDELLKDAAGLRAFLLRNCGRSSPQ"
     gene            complement(8462..9118)
                     /locus_tag="VFA54_04775"
     CDS             complement(8462..9118)
                     /locus_tag="VFA54_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50152.1"
                     /translation="MAASTTTPLRSILLVEDDPDYSSLLREAFSEAGFEILEARNGEK
                     ALQLLRDQKVNLVISDFMMPELNGLELCRLVANDVELSHVRVILYSCNTDALFRRKAR
                     ELGALEYLPKTEDVSALVRQICDVAGLAAARNQAAPEPNGHSRLEDVLQSVHRSTSQL
                     SSLINNLLDFIQIAALTEASPPPVRVAWEAAQRIRADIQQVLGEIEKNMEQLKSAPSS
                     "
     gene            complement(9162..9956)
                     /locus_tag="VFA54_04780"
     CDS             complement(9162..9956)
                     /locus_tag="VFA54_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS50153.1"
                     /translation="MEKAAATPSNLPQDRALSEIDEFVYAISHDLRAPLLNFQGFLQR
                     LERAAEALRGHVETGHLPPDQRRLAEQILNEKIKPSLDVLERNARRMDRLLAALLDLS
                     RTGREPLQMQEVNGSEIAQAVIEEFRAAAAAKNAALVLESMPVLWADPHRLQEIFRRL
                     IDNGLKFLHPQRPGLVTVGGSAGRSEHVCWVRDNGIGIRGRDRDKIFRPFGRVQEISA
                     PGEGAGLAAVRKLIEQQRGRLWVESDHGSGSTFFFALPAKPGHLIE"
     gene            10154..11050
                     /locus_tag="VFA54_04785"
     CDS             10154..11050
                     /locus_tag="VFA54_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50154.1"
                     /translation="MKSQPAVILLVEDDLGDQVLTQEAFKSFKTPYDLRIVPDGKEAL
                     DYLYRKGDYETEAAPEPDLILLDLNMPRVNGQQVAERVHADPRLRQIPIVVLTTSRRE
                     EDVVRLYGRGATTFITKPLDFKQFKATVRDLEYLIKFVAELKNLRDRARVTDAQIRKL
                     TRRRQQLQRLTDGLFDQHMRQIEAIFLENRERLGSAEPQQSVSCTETAAGRALLLLAG
                     KILKGRPEEWKRISGEPAMESSAFHGAGASHGPTEMSEGLSRLARSLNNLSEKSAAAS
                     PSAGQDETNHSTPEAPEHTENR"
     gene            11342..11860
                     /locus_tag="VFA54_04790"
     CDS             11342..11860
                     /locus_tag="VFA54_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZS50155.1"
                     /translation="MDWARLLLALAVFSAAAQSPKTAPAAKAKYMPPPPVQPIPYSHK
                     QHLALGLQCKNCHEMAEPGDYAGLPSTATCMNCHTTIKKDSPSIQKLAQFHRDGQPVP
                     WVRVYHIPDYVDFSHKVHLTRAKATCETCHGPVRERDVLRKETDITMAGCMDCHRANN
                     ASIACDYCHALR"
     gene            11964..12638
                     /locus_tag="VFA54_04795"
     CDS             11964..12638
                     /locus_tag="VFA54_04795"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZS50156.1"
                     /translation="MQPLLHLFKTLTDPWQLVAFLQGWPGYLILFAIVFAETGLLVGF
                     FLPGDSLMFTVGVFAGAGHLNILWVNGILMAAAILGDSFGYLLGRKTGPKIFSREDSR
                     LFHKRHVVRTQQFYQKHGGRTILYARFIPIIRTFAPYVAGVGQMEYKRFIGFNVFGGI
                     GWVLALTMLGYGLGNVPIVKNNFEKMILLIIFLSILPGLIEVLRHKLGAGRRGKSEVP
                     VAADTE"
     gene            complement(12864..13286)
                     /locus_tag="VFA54_04800"
     CDS             complement(12864..13286)
                     /locus_tag="VFA54_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50157.1"
                     /translation="MPLDISISNTTLNAPGNINRPNVNGPVKILGDIGPGQLYFGASA
                     FSAPPPNTFGNLGRNVLHGPRLFSIDFSVFRKFRLTERFNLEFRGESFNVTNTPHFDN
                     PGTNFSNNNFGQVTTAQGNQTVKVNENRQLQFSMRLVF"
BASE COUNT         2599 a         4153 c         4107 g         2817 t
ORIGIN      
        1 cccagaagca catgtcgatg caccaccact cgatgtccgg aatcttcaac aactgcggcg
       61 ccagcgaggc aaaatcgatc ttgccggcgc cgaacggaac atgggtgctg gtttcgttgt
      121 tgtggaggga gccgtcggag tcgatgagat ggatatggcg gatccgactg tccagcaacc
      181 ggagcatctc cggaatgccg ccgggcaaga cctccagcgt tccctggccc cgcgcgccca
      241 tcactgccga catgtaagcg tgcgaggtgt cgaagagaat gccgaagttg gaatgattca
      301 ccgcgtcggc gatcgcgcgg atttccgagg gcttgttgaa agcgaaggcg ggttcgaact
      361 cccaaacgac gcgcacgccg gcggcctgcc ccagatcagc cgcgtcgcgc caggtctcga
      421 cgagcctgtc ccgggcggcg agatattccg cgtccggaat ggaatcgggc gccgcccccg
      481 aatccacgcg aatcgccggg atccgcaagt cgcggcacag ctccaggttt ctctgaaaca
      541 gatctaaata tttttgtttg tttccgggcg ccgccggatt cacagaggta aggtctgcgg
      601 cataaccgga acactcgagc ccgaggtctt gcaagaagac gcgcaattcc cggcgcggcg
      661 cggctgtcgg atagcggttc aggctgacgt gcgggggaaa accgcagacc tcaacaccgt
      721 cgtaacccgc ctcagccagg cggcgggcgg cgcggtcgaa gggaatggga tgggattcat
      781 acggcccaaa cgaaaaagcc caactgccga gagaaatttt catgtttttc tccgtggccc
      841 gatgcgccag cggtagcgga gtgttttcag gccgctccta tcggccgcag ccaccggcgg
      901 aacgccgtct cggccggacc gtaaggccgt tttgccgttc ggtccggtcc tctcacggcg
      961 gttgcggagg agaatgcagc cttctttttt ctgcggcggt gctaggctgt tgatctgggc
     1021 cgcggcggtg agagccgccc cgaaaagcag aagagacgaa aatcgacgca cggttttccc
     1081 tgcctgatga cgcagaatgt tgtacaacgg tttagcgcgc caacacaagc ggcggccatc
     1141 ctcatcctcc tgctccattc aggcgcctgc ggaccggcct ggctggccaa gcaggccgag
     1201 tttgccgtgg agaattgtac ggccctcgat ggagcgccgg aactctcctc ctggcgatgg
     1261 aaagtcgacc ctgatccggt gctgacgcga ggtcctaagg gggcctggga ttcgttcgat
     1321 gtactgaacc cgagcgtagt tttctggaac ggcctctact acaacctgta ctccggcttt
     1381 gacggtcaca cctggcgcac cggcctggct acctcagtgg acggccggca ctgggagaaa
     1441 ttcgccggca atcctgttct ctctccgctt cccggctcct gggaaggcga ttacatcgcc
     1501 gccaacggtt cggctctgca tgacggctcg caattccttt actggtatca tgcaggccgc
     1561 ccagcgcgca tcgggctggc ccgctccacc gacgcccggc actggcagcg gttgggacct
     1621 ccggttctcg agccggggcc tgaggggagt tgggatgagt cgggcgtggc ggacccgtat
     1681 gttctccgct gccgcgacgc gtactacttg tattatctgg ggcagaaccg ccgggctgtg
     1741 cagcgcatcg gcgccgcccg ctcgccggat ggcatccact ggcagaaatt cattggaaat
     1801 ccggtgctcg atccggggcc tccgggagct ttcgacgagc gcggcgtcgg ggaacctgcc
     1861 gtgttccgcg cggccacgga attctatatg atttatacag ggcgcagtgc cggcgagacg
     1921 cgacgcctgg gggcggcccg atcgtccgac ggtgtgcatt ggcagcgggc ggcgctggcg
     1981 gctacgattg cgagcggtca gccgtgggat agtcatgtgg tgtgcgaccc gtccgtgctg
     2041 gcggtcaacg gcaagctctg gctatggttc ggaggcggtg acgttgcccg ccccgacgag
     2101 cgcctccacg gacaaatcgg cctcgcaaca gcttcgctca acgggcgcta agcaatgttg
     2161 tttcgatggc ggtttcgggc cgttgcggct ttttgagtga tccggatcaa gaaacttgga
     2221 ccgcgcgccc gggcggagaa tccaattgca tagcgcaatg aaaaggttca tctacctttg
     2281 cctgctcggc ccctggctgc tgctgggaca gtcgggcgtc cccaatctga agcctgatcc
     2341 cgtccaatgg tcgttgcgtc tcgagcctga ctcagcggcc ccgggtcaaa agatactggg
     2401 ccggctgatt gccacgatcg agccgggctg gcacctgtac tcgctgacaa ctccacgccc
     2461 tccgattgcg acgacagtcg ccgtgcagcc atccccggcg attgaaaaat ggaccgtgtt
     2521 cgccccgcct cccaagcgcg ctctcgatcc caatttcaac gtcgagacgg agacctacga
     2581 gaaaaatgtc gacttcgtga ttgatttgga tctcgcccca agtgcagcgc cgggtgcagc
     2641 gccaattacc gcgcaggttc gctatcaggc ttgcaacgac aaaacctgcc ttttcccggc
     2701 ccggaaaacg gccgccgcga cgctgaaaat cgatcctaac atccgggccg ccaccccggt
     2761 cattcccgcc ggatacacgg aattcaaagg cggaccgccg gccgccgggc cgggaacggc
     2821 aactccgacc ggcggcggcg catccggcgg atctccagtg agtccggctc ccgccgcctt
     2881 tgccggctca caggggaccg gccagttcat cgccctcgct ttaggcttgg gatttctcgc
     2941 catcttcacg ccatgtgtct ttccgatgat ccccatcacc atgtcgtatt tcctgggagc
     3001 gcagacgggc agccgccgcc agagcgtgat ccaagccttt acgttttgtt ttggcgtcgt
     3061 gttgctgttt acgggactcg gcgcggcagt aaccgctatt ctcggcccgt ttggaattac
     3121 gcagctaggc tccagcccgt gggtcaattt gtttattgcc ctggtgttcc tggctttcgg
     3181 tctcagcttg ctgggagctt ttgagatcac agtgccgagc ggcgtgctga ctaccgtaac
     3241 caacgcgtct taccgaggcg gcttggcggg cgctttgatc atgggcttgg cgttttcgct
     3301 ggcttccttc gcctgcacgg ggccttttgt aggaacgctg ctggcgggct cgacccaggg
     3361 cggtctttcc tggccgatcc tcggcatgtt cttcttttcg atcgggctgg cttccccatt
     3421 tttctttttg gccttgttcc cggcttattt gaagcgcttg ccgaagagcg gcggctggct
     3481 cgaacggacc aaggtaacct tggggttcct gatactggct gcgatgatga aatacctgag
     3541 caacattgac caggtttacc agtggaacat cctcacgcgc gagcggtttc tggcggtctg
     3601 gattgtaatg ctgatgctgg ccggactcta tttgctggga attctgaagc tgaaggagga
     3661 aaaagacgag cccgtgagcc tgggccgcct ttttgccgcg gccgctatgc tggtgcttgc
     3721 catttcgctc gtgcccggga tgtttggcgc gcggctcggc gagctcgacg cctacgttcc
     3781 ggctcccggc gagtctgcgc tgaccagcgg cggcgctacg gccgaattga agtggatcaa
     3841 agacgattac cgcgctgctc tcgatttggc ccgccagagc ggcaagccgt tatttgtaag
     3901 cttcacgggc tacgcctgca ccaactgcca ttggatgaag gccaacatgt tcacgcggcc
     3961 ggaaattgcc gctgtcttga agaactttgt gctcgtcgaa ctatacaccg atgggaccga
     4021 tgccgcctcc gaagcgaacc aaaagatggc cgagacacgc ttcaacacca cggcgattcc
     4081 gttttacgcc gtgatccgcc cggatgacac cgtctcgggg acctttgccg gcgtcacccg
     4141 gaatgcaaag gagttcgaag cctttctgaa ccgcgggctc ggcggcgcgg cgacgccaag
     4201 ccgcccgagt gcggctgcgg tgccggctgc cacaccggcc tcttagagta tggcccacgc
     4261 ccttaaggga agtccttacc agggctaagg gttacccccg atgggcggaa acctctaaat
     4321 tccctacact cttgtcgata gaccaaagcg aggctatcga tgatcccccc tctcgatccc
     4381 ggcggtgcgc ttgccgcagc gctatcagct gctgttttca tcgttgccat tgtctacgtg
     4441 gtccgatccg gccacgctga tatccagact cctttgatgt cggcgctggc gggagaagac
     4501 gccctcctcg gccgagaact gttctccaac gccattctgg agcgccgtct tgccggccgc
     4561 ggcctgctgc tttcggtcgc tttacatgct gttgttttgc tggggacccc cttgctgcca
     4621 tatctcttcc cggatcgcct gaactttgat ttccggaaat ataacgtcaa gatcatcgaa
     4681 ttccgaattc cggagcctct actctataca gttccggacg caaagcagag ccgctcccga
     4741 gagcgagccg agccgcctgg ccgcgttgcc cgggggcgca ggaacttgtt ggcgcgggca
     4801 tcccccgcgc caggcagccg cctcccgggg gttgcgggag ggccgcctcg cttacagcta
     4861 ccctcgtcgg cgcaatctcg agcgagagat gtagtcatcg aaccggatca gccgccccag
     4921 gtgagcattg cggcgcctca tccgcttccc acagcttttt tgtgggcgca ggggccggcg
     4981 ccagatcgcc tttcccggct cgtgggcgag ccgcctgcgg tcaaattacc gctctttgcc
     5041 cttcctcatt ccaccccggc ggttcagcgc cccaatcggg aagtagcgct tgcggaatta
     5101 cagatcgcat ccggacccgt attgacggag cggcccccgc aactgcccgt gcttccggcg
     5161 aacatctcgc ctgtccgtat gccgtcacct ccgatcgatg ctccgggcca gttgccggct
     5221 tcggctcttc ccggatcgag ccctttgaac ctgatcgctt tgatgcaaac gccggctcta
     5281 tccgctcccg cttatctttt gcaaaccgga aatcgactgg ccgaggcgca acccggcccg
     5341 aatggcccat cgcaaggggc gtcaggcggg aatggttcgc cagcggccaa cgccaatgca
     5401 tcggagacag ccaaagatgc tcccaaggct ccgggcgccg aaaccagtgc gaatacccgt
     5461 tccgacacgt caggagccga aacagcgccg tctaagggaa cctatgcgga cttgctggcg
     5521 gcatccagcc caggctctac gggaaaagga tccttggccg ccgactcgaa tgccgggcag
     5581 gcaggggaac aggagcggcg caagccctcc gggcagtcct atccaagcgg caacctggga
     5641 gtcatcatcg tgcaacagtc ggcgcaggat acgattctcg aggggagcga agttctgact
     5701 ggccagccgg tttatacggt ctatttcgac gtgccgggcg ccccgcgccg ttggatcttg
     5761 cagtattgcg tacccggctc agactctaag acgttcttcg agcagcccga cggagtgatc
     5821 cgcattttgc ccaaacgctc ggtccagccc ccgtttccga tcgagcgaat cccgctggac
     5881 gtcaagagcg ttccgaatcc cggcactccc gtctccgcgc gccggctcgt catctatgcg
     5941 accgtaaacg agcgcggcga gaccggcaat gtccgcctga tccggggcac ggggcaggaa
     6001 atcgatcgga ccgcaatggc gaccctgcaa cgctggacat ttcgtccggc gatgcgcggc
     6061 gatacgccgg tggccgtcga agcgctgttt ggaattccgc tgccataggg gcaactgctc
     6121 cgtttttctc acgccaacaa atcaagcagt ttctcgtcca attcgttcgc ggcgcgaatg
     6181 agcttgacgt ttgccgtgaa cgccgagcgc gccgaaagca aacgcaccgc ctccttactg
     6241 acatcgattt cggcggccga ggacggatcg gccagccggc tcagattgtc ggcggcctga
     6301 tcgatttgcc ggatcgctgc atttatgccc gccaatgcgg ctccagttac tctcatgtgt
     6361 tacactcatc ggctcgttcg caaatatttc aagggggaag cgcctgccgg atcaactttt
     6421 aactgtatgg aacataaagt cctgatcaca gtgagcccta tctatagcga agctgtcaat
     6481 tttctgacgg cgcgtttaaa aggggcggga ttcgatgtcc agtacctaaa tcaacccgac
     6541 gagcagttga gcgctgccga actgagcgat ctgctgcggg gcgtggaggt ctatgtcgtg
     6601 gggaatgcgc gggtgccccg gcaggcgatc cagagttcac cgcgcctggc cctggtggcg
     6661 aagtatgggg taggcgtgga caatatcgat ctggcagcgg cgaccgatca cggcgtgctg
     6721 gtgaccaatg ccccgggcga aaatgcgatc tcggtggccg aaatgaccat tgccgggttg
     6781 ctcaatctct cccgcaagct ccgcgaaatc gagcgctctt tccggcaggg gaagtggcgc
     6841 gctgttttcg gccacgaaat ataccagcgc acgctgggca ttatcggatt gggcaacatc
     6901 ggcaagcagg tggcgatcag ggcacgggct ttcggcatgc ggattctggc gtatgacctg
     6961 ctggactacc cggaattctg ccgcgagcac caggtcgaga tgactccgct cgatcggctc
     7021 ctggcagagt cggatgcagt caccctgcac gtgccgctta ccccggagac ccgccatatg
     7081 atcggcgcac cccagtttag ccgtatgcgt cccggggctc tgttgattca cacggctcgc
     7141 ggcggggtgg tggatgagca agccctttat ctggctctcg cggacggccc cctgggaggc
     7201 gctgcaatcg atgtatttga atgtgaacct ttaggagaaa gcccgctgaa gaggcttgac
     7261 aacgtcatcc tgacgcccca tgtcgcgggc atcagctatg aggctgccga acgcgtcgcc
     7321 gcacggacgc tcgaaaatgt gctcgcttac caagccgggc ggactcctcc gtgcctgttg
     7381 aaccccgagg ccgcccgcct acgcaagaaa cttgatttcg tcgatagata agcgagaccg
     7441 gatctgccgg cgcaaacctt gcggcaaaag aattggcaaa aacgccgtcc ggcaatgcga
     7501 aaaagagtga gcgagccgcg cttatatcgc gcaacgccgc ctgattcgcc agcccccgca
     7561 gaatgccatg catttgtctg atcgccggcg cgcgccatgg ttaattctgg ttgttgatga
     7621 cgattacggc gatcagatcc tgatccagga ggcgcttcag gccagcggca tttgcaagca
     7681 gatccaaatc gtaagcgacg gggaagaggc catggaatat ctcctcgggc aaggccgtta
     7741 tgcggctccc ggatcgtgcc gccgtcccga tttgattttg ctcgacctga atatgcctcg
     7801 cctggggggc aaggaagtgc tggcgttggt gaaggccgat cagaatttga ggtcgattcc
     7861 agtggtggcg tttacaacct ccggccggca ggaggacatc agccagtgct acagcctggg
     7921 cgtgaattcc tatgtgcaaa agcccgtaaa ttttcaggaa ttccagtgcg tactacgtcg
     7981 tgtagaggaa tattggctgg aaatatcctt gcccccgccg ccggggtaaa ctgacatgat
     8041 acatgcagcg ggcgctcacc attctccata tcgatgatga ctcagacacc cggttcttgc
     8101 tgcgggagct catttctgag tctccggagc cggagggcgg cgagcacacc gaaatctgct
     8161 ggctggacgc ctcgggggtc gaggaagccg tggcccaatt tgcaggggcg ccgctcgacg
     8221 cgatcctgct ggataaccgg ctggccggtg aaaacggcgt cgagctgctg cctcgcatcc
     8281 ggcaggtctg ggactgcaag gtctggatcc tgacgggttt ttttcacgaa aaactacacg
     8341 acgaatctct ccaacgcggg gcggccggag tgatcggaaa agacgagctg ctcaaggatg
     8401 cggccggcct gcgggccttt ctgcttcgga actgcgggcg ctcctcgccg cagtgaacac
     8461 ctcaggaact gggcgccgat ttcaactgct ccatgttctt ctcaatttcc cccaacactt
     8521 gctgaatgtc ggcgcgaatc cgctgcgcgg cctcccaagc gacgcgcacc ggcggcggcg
     8581 aggcttccgt gagagccgca atttgtatga aatcgagcag gttgttgatc agcgaactga
     8641 gctgcgacgt gctccggtgg acggactgca acacatcctc gagccgggaa tgcccgttgg
     8701 gttccggcgc cgcctggttc cgcgcggccg ccaggcccgc cacatcacag atctgccgga
     8761 ccagagcgct gacatcctcc gtcttgggta gatattccaa agctcccagt tcgcgcgctt
     8821 tcctgcgaaa cagcgcatcg gtattgcaag aataaagaat cacccgtacg tgcgagagct
     8881 cgacatcatt agccaccagc cggcaaagct ccaggccgtt caactcgggc atcatgaaat
     8941 cggaaatgac tagatttact ttctgatcgc ggagcagttg caaagccttt tccccattgc
     9001 gtgcttcaag aatctcgaaa ccagcctccg agaacgcttc ccgtagcagg ctggaatagt
     9061 cggggtcgtc ttccaccaag aggattgagc gaagaggtgt cgttgtgctc gcagccatgg
     9121 aaatccctct cttgcgctta tcggcgcgaa atcgggaaag tttattcgat caggtgaccc
     9181 ggcttggcgg gcaaggcgaa aaagaaagta ctgccggacc cgtgatcgga ctccacccag
     9241 agcctgcccc tctgttgctc gatcagcttt cgaacggcag ccagaccggc gccttccccc
     9301 ggagcggaaa tctcctgaac tcttccgaac ggccgaaaaa ttttatcgcg gtcccggccg
     9361 cgaatgccga ttccgttatc ccgcacccag catacgtgct ccgaacggcc ggccgagccg
     9421 cccacggtga cgaggccggg gcgctgggga tgcaggaact tcagcccgtt gtcaatcaag
     9481 cgtcggaaga tctcttgcag ccggtgcggg tcggcccaca agacgggcat agattccagc
     9541 acaagcgcgg catttttagc cgccgcggcg gcgcgaaact cctcgatcac cgcctgggca
     9601 atctccgatc cgttaacctc ctgcatctga agcggctcgc ggcccgttcg cgaaaggtcg
     9661 agcagcgccg ctagcaggcg gtccatacgg cgcgcgtttc gctccagcac atccagcgag
     9721 ggtttgattt tttcgttgag tatctgttcc gccagccgcc gctggtccgg tggcagatgg
     9781 cctgtttcga cgtggccccg gagtgcttcg gccgctcgct ccaggcgctg gagaaatcct
     9841 tggaaattca gcaggggcgc gcgcaggtca tgggaaatgg cgtagacgaa ctcgtctatt
     9901 tccgaaagcg cgcgatcctg cggcaagttt gagggggtgg cggccgcttt ctccatacat
     9961 ccaacccatc ggcccgaatc gcagagggct acagtgtgct gtttcgctgc ggagcgttgc
    10021 aacttccgcc cgcaagagcc gatgagttcc ctggagggtg gctcctggaa attgggcccc
    10081 tcgtctgccg gatttgaacc gagcggcccc gcgcagctcg ggcgtttgcg gaggtttgat
    10141 ggtgagtacg gccatgaagt cgcaaccagc ggtcatcctt ttagtggaag atgatctcgg
    10201 cgatcaggtc ttgactcagg aagcttttaa aagcttcaaa acgccctatg acctccggat
    10261 tgtgccggat ggcaaggagg ctctcgatta tctctacaga aaaggcgatt acgaaacgga
    10321 agccgcccca gagccggatc tgattctcct cgatctgaat atgcctcgcg ttaatggcca
    10381 acaagtagcc gagcgggtcc atgccgaccc gcgcctgcgg caaattccca ttgtggtgtt
    10441 gaccacatca cggcgggagg aagacgttgt gcggctctat ggacgtggcg caactacttt
    10501 tatcaccaag cccttggact tcaagcagtt caaggcaacc gtgcgggatc tcgaatatct
    10561 gatcaagttt gtcgccgagc tcaagaacct ccgcgaccgt gctcgcgtca ccgacgctca
    10621 gattcggaaa ctcacgcgac gccggcagca attgcagcgc cttactgacg ggcttttcga
    10681 tcagcatatg cgtcaaatcg aagccatttt tctcgagaat cgcgagagac tgggatccgc
    10741 cgagccgcag caatcggttt cgtgcaccga aactgcggct ggccgcgctt tgctgcttct
    10801 ggccgggaaa atcttgaagg gccggcccga agagtggaag cggatttcgg gagagccggc
    10861 catggagagt agtgcgttcc acggagccgg tgcgtcccat gggccgaccg agatgtccga
    10921 agggctctcg aggctggcgc ggagcctgaa taacctgtcc gagaaaagtg cggctgcctc
    10981 accctcggcg ggacaggacg aaaccaatca ttccacgccg gaggcgcctg agcacacaga
    11041 aaatcgctaa atgttctgat tcccgagctt gatcccggct taaaaacccg tcagatcgat
    11101 tgaccggccg gcgaagggcg cggctcgctg ggctgccgcc ggctcgaatc caggaaacgg
    11161 cgcagccctg gttccggcgc acggatttga ccgagagcct ctgcgcggaa tcccgacacg
    11221 actggattag cccgtttctg ctgtttcgtc tttgtcgatc gcccggcgac ggtggcttcc
    11281 agtcccgcgt aagagccgga caatcccttt catccacaag ccatgcgcta tgattgatgt
    11341 catggattgg gcccgactct tgctagcgct cgctgttttt tccgcggctg cgcaatcgcc
    11401 gaaaactgcg ccggccgcca aggcaaaata catgccgcct cctccggtgc agccaattcc
    11461 ctacagccac aagcagcatc tagcgctggg actgcaatgc aaaaattgcc atgagatggc
    11521 cgagccgggc gattacgccg gcctgccctc gacggcgacg tgcatgaatt gccatactac
    11581 gatcaagaaa gacagcccat ccattcaaaa actggcccag ttccataggg acggccagcc
    11641 cgtgccttgg gtgcgggtct accatattcc cgactatgtc gatttcagcc acaaggtgca
    11701 tctgacgcgc gccaaagcca cgtgtgagac ctgccacggg cctgtgcgcg aacgcgatgt
    11761 cttgcggaaa gaaaccgaca ttacgatggc cggctgcatg gattgccatc gcgccaataa
    11821 cgcttcgatc gcctgcgatt actgccacgc tttgcggtga gaatggctta taacagcctt
    11881 tgaaccggtt ccgctgttcc gttcggaccg gtttcctgcc atgcgcctca gtcgatgagg
    11941 gcgccaatgc tacactcaga tgagtgcagc ctctccttca tctctttaag acgctcaccg
    12001 acccttggca actggttgcg tttcttcagg gctggccggg ctatctgatc ttgttcgcga
    12061 ttgtttttgc cgaaacgggg ttgttggtag gctttttcct gccaggcgat tccctaatgt
    12121 tcacagtggg cgtcttcgcc ggcgcggggc atctgaacat cctttgggtc aacggcattc
    12181 tgatggccgc cgctattttg ggagacagct tcggttattt gctgggtcgc aaaaccggac
    12241 ccaaaatttt ctcgcgcgag gattcccggc tcttccacaa gaggcacgtc gtccgcacgc
    12301 agcagtttta ccagaagcac ggcggccgta ccatcctgta tgcccgtttc attcccatca
    12361 tccgtacatt tgctccttac gtggcggggg tgggacagat ggaatataaa cgcttcatcg
    12421 gattcaatgt gtttgggggc atcggctggg tgttggctct gaccatgctc ggatacgggt
    12481 tggggaacgt tcccatcgtg aagaacaact tcgagaagat gatcctgctg atcatcttcc
    12541 tctctattct tcctggtctt attgaggttc tgcgtcacaa gcttggcgca ggccgccgcg
    12601 ggaaaagtga agttcccgtg gccgccgaca cggagtagcg catcgccttc ccgaatgccg
    12661 gaagcgcgcg gcgatttccg ctgaatatgt tttcctctgt gcgggccaag caggctgttt
    12721 ccccggcccg cctgttgggc ccgcagagtc aaaaccgcag gcgccggacg tacggaaacc
    12781 ggctgccaag caagaacggc cggctggcgg atatttcaat ccgaaagccg gccgatttgt
    12841 tggagggctt gtggcgcggg ccgtcaaaat accagccgca tgctgaattg cagttgacgg
    12901 ttttcgttga cttttaccgt ctggttgccc tgagccgtgg tcacctggcc gaaattgttg
    12961 ttactgaagt tcgtccctgg attgtcgaag tggggcgtgt tggtcacgtt gaacgattcg
    13021 ccgcggaact ccaggttgaa ccgctccgtc aggcggaact tccggaacac cgagaagtcg
    13081 atggaaaaca gccgcgggcc gtgcaggacg ttgcgcccca gattgccgaa cgtatttggg
    13141 ggaggcgccg agaacgccga ggcgccgaag tagagctggc ccggcccgat gtctccgagg
    13201 attttcacgg gtccgttcac gttggggcga ttgatattgc ccggagcgtt cagggtggta
    13261 ttgctgatgc tgatatccag cggcaacccg ctttcccatg tccacagccc attcagttgc
    13321 cagccgccca gcagccactt ggaaatcccc gactgcgccc agcgttttcc ctgcccgaag
    13381 ggcagctcgt aaatggcgct ctcggtgaag acgtgccgcc gatcccactg cgccaggcct
    13441 ttgttgagcg ccagattgat gttgttgaaa ttcccgcccg atgtccggcc atcgaagtcg
    13501 atgcctttcg cgaaggcata ggacgttgtg agcagaaacc cgttagcgat tgtccacccc
    13561 ttcgatcatg tagttgttgt gctggtccag gccgttcacc acggactgca aatgctcctg
    13621 ggaattaaaa aacggcgagt tggagcgata ggcccgttgc acaccgggaa tcagca
//
LOCUS       DASREK010000093        23180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1702740, whole genome shotgun sequence.
ACCESSION   DASREK010000093 DASREK010000000
VERSION     DASREK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 23180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23180
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702740"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFA54_04805"
     CDS             complement(<1..482)
                     /locus_tag="VFA54_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50158.1"
                     /translation="MDCLSPIKVLAWMAAGALVIVAGGALGSPAGRRNKQSGAREESG
                     ETEDSEQFEDLVDRPVAQAILSESIEAVHRIESLAGSVAALEARMQALERSSPAGRLD
                     EIWQRVLQIEERLESIEAERQAAPSLDALVAEAGKRLSSGMSAIESRVEQQQAAIEQL
                     "
     gene            complement(540..1553)
                     /locus_tag="VFA54_04810"
     CDS             complement(540..1553)
                     /locus_tag="VFA54_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZS50159.1"
                     /translation="MKCAVFYGPGDLRIEQRPKPQIRQDGDVLLRVEAASICGTDLHI
                     LADPPGHPATPGSILGHEYVAEVLDAGDGVDGLRVGSRVVVDPNITCGRCAYCRRGAP
                     NLCRNMTTLGIFRDGGFAEYNVAPAASLYPISKDIPLERAVFAEPLSCVLNGFGKAGL
                     KIGDNAVILGAGPIGLLFSMLFRAGGAGKVGISEVSPVRRRLASLAGADQVWNPQEVS
                     LPESVREWSGIGADIVVDSVGSLLGEALRLVRSGGRIVLFGMNQQAESAVNQYEVTRR
                     EISIAGSFIAGGTFPQTVATLENPPLPLESIITHRIGLDGINSILPAMQSGEAVEVVI
                     RPE"
     gene            complement(1804..2196)
                     /locus_tag="VFA54_04815"
     CDS             complement(1804..2196)
                     /locus_tag="VFA54_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50160.1"
                     /translation="MDYRIADTNPDLFAPHRNLSDDVNGKIVKSEIEGGVYLDDLPRG
                     AEIEVETQNRFYRIVNRGGGSALISGHPQFCPSPVLVKIHGSNWGGSMLKLRFIGRGM
                     HLEFLHPRFRTIITSRIVEIRPLAACTA"
     gene            2285..4243
                     /locus_tag="VFA54_04820"
     CDS             2285..4243
                     /locus_tag="VFA54_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50161.1"
                     /translation="MPKTAAAAKKPRREALEKPYPAKLSKDQAQTVTSWHGAAVVAAI
                     TLAVFWPAIGNQFVNWDDDKNFLNNPYFRGLTLSHLRWMFTTSLLGHYHPFTWITLGL
                     DYVLWGMNPAGYHLTNILLHAANAVLLYLIALRLLGGRIWVAVLAALLFSVHPLRVES
                     VAWVTERRDVLSGFFYLLSVLTYLRAQRPPEDGPSSRTREMALSAAFFAAGLLSKVIV
                     ASLPLVLLLLDAYPLRRGFRLAEKIPYFLFAAAGALAALATQGAGVHGFAEHVVLLPG
                     LRTGLSLYGLFFYLWKTVLPFGLYPQYVLPAGISVFDRRIVLAAALAAALTITAVVLR
                     RRLPAFAAVWACYVVTLLPVLSFARLDTQQYAADHNSYLATLGLALAGAAAIAAIRRR
                     EIARAAAPLLILIFAMLCRRQIPVWRDPSSLWTATLAGAPDSVVAHNNLGEALAAQGR
                     LGEAAAHFRRAIEIRPGYAQAHYNLARASQKQGDLPGAIAEFQRALQIEPAFATAHND
                     LATCYAALGQSGPALEQYRLALRYQPDYADAHYNLGNLLQQQRQLEDSIAEYRQALRY
                     NPGLADAHNNWGVALDALGRPAEAMEHYRQALLIAPTNSDAHNNLGAALEAQGKKDEA
                     VAEYRQALRFNPGHRDAAANLARALRAP"
     gene            4597..5961
                     /locus_tag="VFA54_04825"
     CDS             4597..5961
                     /locus_tag="VFA54_04825"
                     /inference="COORDINATES: protein motif:HMM:NF005812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HZS50162.1"
                     /translation="MAATLSATSRDLDLQHLWHGNLQHRDLAKRPPLEITRAEGCYVY
                     DAAGRRYLDAMAGLFCVNVGYGRREIVAAVADQMSRLPYYPLTQSHGPGARLAARLAG
                     LLPGELGRVFFVNSGSEAVETALKIARQYGRQAHPGQNRYKIIARNRGYHGFTMGAAS
                     ATGQILRKQAFEPLVPGFFHVSPPDHYRCPHCSREPACTLACADEVEQVIRAEGPETV
                     AAVIAEPVIGGGGVFPAPEGYWERLREICDRYEALLIADEVITGFGRTGKLFGFEHAG
                     IVPDIITIAKGITSAYLPLAAAVATERVFQIFQAGESDEAKFTQVSTFGGHPASCAAA
                     LANLDILVGERLWENAARMGSYLLEALRAIHSPFIGEVRGRGLLIGLEMVESQDTRTP
                     LPEAAAAGLQRAIRDAGVIVGRNSDTVPGFCNVLILSPPLTLSREQADAIVEALEAGL
                     AQLH"
     gene            6101..7615
                     /gene="accC"
                     /locus_tag="VFA54_04830"
     CDS             6101..7615
                     /gene="accC"
                     /locus_tag="VFA54_04830"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZS50163.1"
                     /translation="MAKTPFRKILIANRGEVAVRVIRACREMGIASVAVYSEADRAAL
                     HVRMADEARAIGPAPAPESYLSVERIIEAARASGAGAIHPGYGFLSENPALARACDEA
                     GIVFIGPSAGSMELMGSKTAARQRMLAAGVPVVPGATAAAADWRQAQAEARRFGYPIL
                     VKAAAGGGGKGMRVVEREEELPAAMREAASEAERAFGDPSVYIEKYLSRPRHIEIQVL
                     GDRYGELIHLGERECSLQRRHQKVIEECPSPLVAEHEEMRRKMGETAVRAARAAGYYN
                     AGTIEFLVDEDLNFYFLEMNTRLQVEHPVTEMVTGIDLVREQILIAAGEPLGRRQEQI
                     GWSGAAIECRVYAEDPSNNFFPSPGRIASMSEPAGPGVRVDSGAYPGWTVPIEYDPLF
                     AKLAVWAPSREGAIARLRRALDEYHVSGIQTNLGFFREIVNDPEFQAGRLDTGFIARW
                     MSRAKTPPLESCDQERAVALAAALYALRRENSAASASEAAPSRWKTQGRARLLQ"
     gene            7612..8169
                     /locus_tag="VFA54_04835"
     CDS             7612..8169
                     /locus_tag="VFA54_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZS50164.1"
                     /translation="MKLEIIIDGRARAVEVPDQALAGGPGNFVIESSPIQGDIREIEP
                     GLYSVLIAGRSYEVRIDPASAWSAGTEPAARPGTDGPAPDYYAHVRGRRYAVQVRDPR
                     RLPLQRGRPAAAGRQSVASPMPGKVVRLLVSEGEQVKAGQGLLVVEAMKMQNEIRAPK
                     DGRVTAIRVAEGAAVAGGETLAEVE"
     gene            8252..9163
                     /locus_tag="VFA54_04840"
     CDS             8252..9163
                     /locus_tag="VFA54_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZS50165.1"
                     /translation="MREFDVVTGAFGYTGKYITARLLAMGRAVRTLTAHPDRLNPFGG
                     KVSVAPFHFEVAGKLAASLQGADTLYNTYWVRFSHGKVTFDQAVRNTRKLIEAAGTAG
                     VRRLVHISISNPSEDSPLPYFRGKAILERAIAESGLSYAIVRPTVVFGREDILINNIG
                     WLLRRFPVFLIPGSGQYCLQPVFVEDVADIAIAAACAEQNGVWDAAGPEVYVFEELVR
                     AIAAAVRSRARLIRAPAGLVALLCRLLGYFVGDVLLTRDEVKGLMAGLLVSHAAPAGR
                     KKLSEWLAENSETVGAAYASELQRHYR"
     gene            9461..9537
                     /locus_tag="VFA54_04845"
     tRNA            9461..9537
                     /locus_tag="VFA54_04845"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9495..9497,aa:Val,seq:tac)
     gene            9838..10464
                     /locus_tag="VFA54_04850"
     CDS             9838..10464
                     /locus_tag="VFA54_04850"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS50166.1"
                     /translation="MRHAVLWTVNILLLFVLEAAARGQTPPNTLSAEEMQEGWILLWD
                     GKTLNGWESHGSGVWKVEDGALVGEPEGKAGWLGTMQAFSDFVLKAEFRTTADCNSGI
                     YLRAAREGVPRQTGYEIQIRDYKEPQETVPFLTGSLVMHAHASGAKIIPNQWNRYEVT
                     AQGDHFIVVYNGQTVLDTRQSKTSSGVIGLEYNNRKIEFRNLKLRPLR"
     gene            complement(10671..11171)
                     /locus_tag="VFA54_04855"
     CDS             complement(10671..11171)
                     /locus_tag="VFA54_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZS50167.1"
                     /translation="MARNAVLLAIFTVLTAAAAGDEDCFSPPPPQPLPFSHKKHAATG
                     LRCTECHAMEKPGYSAGLPSTATCMACHITIKKESTPIHRLAAYDKQHKPVPWEPVYR
                     LPDYVEFSHAEHVKKGKVECQTCHGLVQERDVTCRETDLAMAACIDCHRTRGGPVGCG
                     SCHDPR"
     gene            11322..11810
                     /locus_tag="VFA54_04860"
     CDS             11322..11810
                     /locus_tag="VFA54_04860"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50168.1"
                     /translation="MPKEARHLVSFIATPQPVVEKMLGLAKVEKDDVVYDLGCGDGRV
                     VIAAAKKYGARAVGVDINPERIRESEENARLAGVAEQVIFRNEDLFEAGIAEATVVTL
                     YLVESLNRKLRMKLWRELRPGTRVVSYNFDMGDWEPEKEIAIEGRKLYLWRIPFHPPT
                     RP"
     gene            11920..15201
                     /locus_tag="VFA54_04865"
     CDS             11920..15201
                     /locus_tag="VFA54_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZS50169.1"
                     /translation="MSHLVLVEVSGMNRIHNSKHLLHAPALLVLGVITAGAASGEVSF
                     NRDIRPIMADTCFRCHGPDKNARMMGLRLDIREEATRRTATGVIPIVPGKPEESAIIQ
                     RIFAKDPARRMPPVYAHKELTETQKETIRRWVAEGAKYEGHWAYQPVRRPEAPAVTQA
                     LAPIRNPIDAFIQSRLAREGLRPSPEADRRTLLRRVTLDLTGLLPTPEEGSAFLKDQS
                     PDAYEKVVDRLLASPQYAEKQAMHWLDAVRYADTAGFHSDLPYPIWPYRDYVLRAFAE
                     NKPFDQFTREQLAGDLLPNATIEQKVASAYNRLNRMSPEGGLQPKEYLAKYGADRVRT
                     TSTVWLGSTLGCAECHDHKFDPFLSKDFYSMKAFFADILETGRAPDIGPGAWGSKLDL
                     PTPEQQRQREQWSWQLAQARRDLDAEAQVLAADTNWEAEVLRRYEAGELDWRYQRPVS
                     AESAHGARLTIYNDEPVESNYYIGGTLVSERAPGRGLVVVSGPNPDNETYTVSFRPGE
                     GAWTALGVEAVHDESLPSHRLARGSDRLVLTEVEAQVSEGASGPVRKLSFVLATPSMR
                     IEPPELHPMAVIDGDPRTGWGVASNGDPGNLFLALRFAQKLHTTADSVVTVRLRHDSQ
                     FRRATLGRFRLALSAGEYSWPEPENLRARLKKETGDPPPSNHGGVPARVLTALRTPED
                     KRTEEQKRTLLDYYEWLTPDLEHRLAQIGKLEAKLGQLESQIPRVVVTEATRPRVTRI
                     LPRANWMDDTHEIVEPAIPVFLGKLETGGRRATRLDLANWLVSPRNPLTARVFVNRLW
                     REFFGAGISKTLDDLGSQGEWPVHPELLDWLASEFMQPAWQAQGAHPWDVKHIIRTIV
                     TSHTYRQSSLPRPEVEERDPDNRLLARQSRFRVDAEIVHDIALEVSGLLVEKFGGPSV
                     RPYQPDGYLAALNFPKRDYSASRGEDLYRRGVYIFWQRTFLHPSLLAFDAPTREECTV
                     NRVASNTPLQALDLLNDPIYVEAARALAQNMIQNGGRTTETRIQWAFERALTRPPRAE
                     EQRILADLYTKNLRRFQAAPMEAKKLIATGETPAPQRIEPAELAAMTTVARAILNLHE
                     TITRN"
     gene            15211..16638
                     /locus_tag="VFA54_04870"
     CDS             15211..16638
                     /locus_tag="VFA54_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZS50170.1"
                     /translation="MDRRDLFTDIRRRTFLSRGVAGLGLLALNSLLDPRLFAAASDLQ
                     SKGAVNPLHHPAKAKRVIFLYQAGGPSHLETFDYKPKLAEMNGKPMPESLTKGQQIAQ
                     LQGKPLICFGPQHKFQKFGKSQQEICSLFPHIGSVADDICIVRSMWTEQINHDPAHTI
                     MNTGSILPGRPSMGSWIFYGLGSEAEDLPGFVVLTSAGRGGQMQPIAARQWSAGLLPG
                     KFQGVKLNSVGDPVLYIENPAGVSAEVQRDSIEAVNKLNRLEYDSVKDPEILTRVAQY
                     EMAFRMQTSVPSLVDMSQEPKQILEMYGAHPGDGSFASNCLLARRLAERGVRFIQLYH
                     RDWDHHDAVKQNVALKAEEVDRPTAALIQDLKQRGMLDDTLVVWGGEFGRTPMSQGGD
                     GRDHHIKGFSFLLAGGGIQGGLTYGSTDDLGYAAQENPVSVHDFHATMLYLLGIDHER
                     LTIKFQGMDARLTGVSGRVVKEILA"
     gene            16707..17105
                     /locus_tag="VFA54_04875"
     CDS             16707..17105
                     /locus_tag="VFA54_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZS50171.1"
                     /translation="MATLRLIEYADASPEVREVYDDIMATRKTDWINNFWKALARDPV
                     TLRRTWQSIKEVMAPGALDALTKEMIYLAVSATNQCAYCIASHTAGARKAGMTDQMFA
                     ELMAVTGMANETNRLASGYQVEIDEKFKNP"
     gene            complement(17217..18338)
                     /locus_tag="VFA54_04880"
     CDS             complement(17217..18338)
                     /locus_tag="VFA54_04880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZS50172.1"
                     /translation="MGLAQEAVLETPSAPAERPRADETPYLAAKRRYLPALTGLRFFL
                     ALWVILNHLMDTGTELDAWSLRLPHPMYVILHSSYLAVGIFFVLSGFVLARGYGSMVW
                     NRGSFLRYGAGRIARIYPGYALSLLIVAPFIIQDRVPGKAGLVARYGLLLQGWAGTPP
                     VHWNTPAWSLSCEIFFYLCFPLGAVCLSKISWRNALAIGAAVCFLPVALLRLGVPDVW
                     KPVIHLADFIMGILTARAYELLLRSRRPFTGRGHWLYVPAILLSAFFILNPETIRGPI
                     DLNGALRPLNALLLIGLSLEGGVLARWLSSRIMVFLGQTSYSMYILHIPLLWWYRRLA
                     AEPTPAVYVPAVVIISAAVFRMLEQPANRRIRRWCDSRI"
     gene            complement(18516..19919)
                     /locus_tag="VFA54_04885"
     CDS             complement(18516..19919)
                     /locus_tag="VFA54_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50173.1"
                     /translation="MSGGVEPVVLVVDDTQASRYVTSCALRQAGFQVREAATGTEALA
                     QAKTDRPEAVILDANLPDISGVQVCRQIKADPETASIAVLHVSAACAAVPAAGAEGGA
                     DAYLVAPVDHTALTAILKALLRAREAEEKAQSMACDWEATFNSVHDAVALVDAGGKIR
                     RCNEKFKLIFGEAAGVLPGRHYQDIVPACQQNPLTRALRTKAHETSRQLIRDRWFRAS
                     AHPIAGGNGITGAVWILCDITDLRHAEELRHRDERLGALTAALITAQEEERRRLSREL
                     HDSLNQKLCMLAVGVEQLEQELPDSAAAIRKELQSLRRRAEEISDDVRRTAYQLHPSI
                     LEHLGLGVALRSYCEEVQKRTGIKIRFRQRGLPDSIPQDVALCFYRVAQEAIQNAAKH
                     SGAQMIVISLRSAGGSLRLTVADSGKGFDPGDKSRQGLGLISMAERVRLVNGALVVKT
                     GPGEGARIDLRVSLQRI"
     gene            complement(20210..20455)
                     /locus_tag="VFA54_04890"
     CDS             complement(20210..20455)
                     /locus_tag="VFA54_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50174.1"
                     /translation="MSREKLSEELEKARLAHRAALDRLESATALSRDLAGTPDSTAAL
                     LRANDEFFSANRKYRAALIAYTEYITREIEERSRGKI"
     gene            complement(20499..>23180)
                     /locus_tag="VFA54_04895"
     CDS             complement(20499..>23180)
                     /locus_tag="VFA54_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZS50175.1"
                     /translation="QFRGHTSEYLEPTPGEASLNLLRMAREGLVLVLRRLIQAVAEQN
                     VSMSETGIYVDHSGEQRQVNIEVTPIAGVAPGERYYLIVFQEGAKKSAAPPLQFNVGQ
                     RSSSLEDYTEALEEHARQLQQQLAETREYLRNLTEDHEAHAEELRAANEEVRSANEEL
                     QSTNEELSTTKEELQSANEELTTVNEELQHRNQELAALNSDLKNLFSAVSIPIVMVDN
                     SLRLRRFNTPAEKLLELGPADVGRPIGHLRGPLEPLPLEKLIVGVLETLKPEQQDVQD
                     QNGRWHSVTVRPYRTTDDRIEGAVITIVDIDPIKRSLRAAEEARDYAEGMIETVREPL
                     LVLDGDLRVQRATLAFYEMFQVSREETEGRLLYDLGNGQWNRQRLRELLGDALFRGIP
                     FQDYEVEHDFPHIGRRTMYLSARRIPRLEARTVLLAIEDVTKRRQEAEIRYGRLFEAS
                     KDGILLIDVEAGSIMDINPYMLELTGYPREEFVGRRLNQTAPFRDAPFSRQLMQELME
                     KDVVRYDDVPLIARDGRRLELQITANRYLVGTRQVIQMNVRDTTDRAALRESQRSFRL
                     FVESVRDYAMFQMDPSGNITSWNAGAERILGYTETEILGQPLSRLFTREDTGRGEPER
                     LLEKARTQKSAEDERWFSRKDGSRFLANAILTSVRDETDKLRGFAMVMRDTTQQRQAE
                     ERLRRSLQEKDVLLKEIHHRVKNNLQMIVSLIQLQSDYLRDPEAAAMFQEMHHRVQSI
                     AAIHELLYGSPDLAHIDFGGYIQKLARDLFEFYRAHPDRIRLRTDMRNTELELNQAVP
                     VGLIVNELLSNCLKHAFPKERSGLVQVTLECGDSQCVLSVADNGVGLPANLDPAHATS
                     MGLQLVRLLVEQLQGTLQLDRSQGTRFTISFPRKIQQ"
BASE COUNT         4416 a         7129 c         7334 g         4301 t
ORIGIN      
        1 tgcaattgtt cgatagcagc ctgctgctgt tccacgcggc tctcgatcgc ggacatgccg
       61 gaagagaggc gcttcccggc ttcggcgacc aacgcgtcga gcgaaggagc ggcctgccgt
      121 tcggcctcga tcgactcgag gcgttcttcg atttgcagaa cccgctgcca gatctcgtcc
      181 agacggcctg ccggcgagga acgctcgagg gcctgcatgc gcgcttccag cgccgccacg
      241 ctccccgcaa gtgactcgat gcggtggacg gcctcgatgc tctccgaaag aatagcctgg
      301 gccacgggcc tgtccaccaa gtcttcgaac tgttcggaat cttcggtctc gccggattcc
      361 tctcgcgccc cagattgctt gttccggcgc cccgccgggg aacccaaagc ccctcccgct
      421 acaatcacca gcgcgccggc agccatccag gccagaactt ttatagggct caggcagtcc
      481 atgaatcatc ctcgatcggt taccaaacaa acccacttta tcatgttttt agcaaaaagc
      541 tattccggcc gtatgacgac ttccactgct tcgccgctct gcatggccgg aagaatactg
      601 ttaatgccat ccagcccaat gcgatgggta attatggatt cgagcggcag cggggggttt
      661 tcgagggtgg caaccgtctg cggaaacgtc ccgccggcga tgaacgagcc agcaatgctg
      721 atctcccgcc gggtgacctc atattgatta accgccgatt cagcttgctg gttcattccg
      781 aaaaggacga tgcgccctcc ggagcgaacc agccgaagcg cctctccgag cagagacccg
      841 accgaatcga cgacaatgtc ggcgcctatc ccactccact cccggacgct ctccggcagg
      901 gaaacttcct gagggttcca cacctggtcc gctccagcca ggctcgccag gcgcctgcgg
      961 accggagaga cctccgatat gccgactttc cccgcgcctc cggcccgaaa cagcatggag
     1021 aacaacaggc cgatcgggcc agcgcccagg atgacggcgt tgtctcctat tttcaggccc
     1081 gctttgccga atccattcag cacgcacgag agcggctcgg cgaagaccgc ccgctcgaga
     1141 ggaatatcct tgctgatcgg gtaaagcgac gctgcgggcg ccacgttata ttcggcgaat
     1201 cctccatcgc ggaagattcc caaggtcgtc atgttccggc acaggttggg cgcgccgcgg
     1261 cggcaatacg cacatctgcc gcaggtgata ttggggtcca ccacgacccg gctgcccacg
     1321 cgcaggccgt ccacgccatc gcccgcatcg agaacctccg cgacatattc atgacccaga
     1381 atgctccccg gcgtggccgg atggccgggc gggtccgcca gaatgtgcaa gtcagtgccg
     1441 cagatgctgg ctgcctccac ccgcagaagg acatcgccat cctgccggat ctgaggcttg
     1501 ggccgctgct cgatccgcag atcgccagga ccgtaaaaaa cggcgcattt cataatcact
     1561 ttctcaggcg ccagggtaac acgcgccgca tcggggaaaa agagctagct tcgaaaccaa
     1621 cgttgcggcg cgctgcggac gttttccggg cggcgatgac gcttcaagag ttcctctgga
     1681 agcggagatc tcaatagtac cgggcggcct ctgctcggga tggccaaacc ggcgaaggac
     1741 gctggaagcg aaaacgggca gcacagtctc gtcaggcgcg ccaggtccca aagcaatttc
     1801 ccgctacgcg gtacaggcag ccaggggccg gatctccacg atccgcgatg tgatgattgt
     1861 acggaagcga gggtggagaa attccagatg catgccccgg ccgatgaaac ggagcttcaa
     1921 catcgagccg ccccaattgg acccgtgaat cttcaccaga accggactcg ggcaaaactg
     1981 cggatgcccg gaaatcaggg cgctgccgcc gccgcggttc acgatccggt agaatcggtt
     2041 ctgcgtttcc acttcgatct cagcgccgcg cggtaaatcg tcgaggtaaa cgccgccttc
     2101 gatctctgat ttcacgatct tcccgttcac gtcgtcgctc agattcctat ggggagcgaa
     2161 cagatcggga ttcgtatcgg cgatccgata gtccattctg agtcgattct actatacttc
     2221 tgcgaagagt ccagcctgct tctccccggc ttttgctcac ctgttagggc gctagaatat
     2281 gggggtgcca aagactgcgg cagcggcgaa gaagccccgc cgggaagcgc tggaaaagcc
     2341 ttatcccgcc aaactatcga aggaccaggc ccagactgtc acgtcgtggc acggggcggc
     2401 ggtggtcgcg gccattaccc tggcggtctt ctggccagcg atcggcaacc agttcgtcaa
     2461 ctgggacgat gacaagaatt tcctgaataa tccgtatttt cgcgggctga cgctgtccca
     2521 tctccgctgg atgttcacca cctctctgct cggacattat catcccttca cctggatcac
     2581 gctgggcctg gattacgtgc tttggggcat gaatcccgcc ggctaccatc tgacgaacat
     2641 tctcttacat gcggccaatg ccgtgctgct ctatctgatc gctctgcgcc ttctaggcgg
     2701 gagaatttgg gtggcggttc tggcggcgct gcttttctcc gttcatcctt tgcgggtgga
     2761 atcggtggca tgggttaccg agcgccgcga cgtgctgtcg ggattttttt acctgctctc
     2821 ggttctcact tacctgcgcg cacaaaggcc ccccgaagac ggccccagct cccggacgcg
     2881 ggagatggcc ctgtcggcgg cgtttttcgc cgcgggactg ctttccaaag taattgtggc
     2941 gagcttgccc ctcgtgttgc tgctgctcga tgcatatccc ttgcgccgcg gattccggct
     3001 cgccgagaaa attccgtact ttctgttcgc cgcggccggg gcgctggcgg cgctggcgac
     3061 gcagggggcc ggcgtccatg gcttcgcgga acacgtcgta ctgcttcccg gtctgcggac
     3121 ggggctgtcg ctctacggtc tgtttttcta tctctggaaa acggttctgc ctttcggcct
     3181 gtatccgcag tatgttctgc cggccggcat ttcggtcttc gaccggcgca ttgtcctcgc
     3241 cgcggcgctg gcagcggcgc tgacgatcac cgccgtcgtc ttgcgaaggc ggcttcctgc
     3301 ttttgcagcc gtctgggcgt gctatgtggt gacgctgctg cctgtgcttt ccttcgcccg
     3361 gctggacact cagcaatacg cagcggatca caacagttat ctcgccacgc tcggcctggc
     3421 gctggcgggg gcggccgcga ttgccgccat ccggcgccgc gagattgcca gagcggcggc
     3481 tccgttgctc atcttgatat tcgccatgct ttgccggcgc caaatcccgg tgtggcggga
     3541 tccttcgagc ctctggacgg ctacgctcgc cggagcgccc gattcggtag tggctcacaa
     3601 caacctcggc gaggctctgg ccgcccaagg caggctcggc gaggccgccg cgcatttccg
     3661 gcgggcgatt gagatccggc cgggctacgc tcaggcccat tacaatctcg cccgagccag
     3721 ccagaaacag ggggatctcc cgggggccat cgcagagttt caacgggcgc ttcaaattga
     3781 gcccgccttc gcgacagctc acaacgacct cgccacctgt tacgccgccc tgggccaatc
     3841 cgggccggcg ctcgaacagt accggctcgc gctgcgctac cagcccgatt acgccgatgc
     3901 gcactacaat cttggcaatc tgttgcagca gcagcgccag ctcgaagact cgatcgctga
     3961 gtaccggcag gcgctgcgtt acaaccccgg gcttgcagac gcccacaaca actggggcgt
     4021 ggctctggac gccctgggac gcccggctga agcgatggaa cactaccggc aggcgctttt
     4081 gattgcaccc acaaattccg acgcccacaa caatctgggc gcagcgctgg aagcgcaggg
     4141 gaaaaaggac gaagctgtcg ctgagtaccg gcaagcgctg cgtttcaatc ccggccaccg
     4201 ggacgccgcc gcgaacctgg cgcgagcgct ccgcgcccca tagcgctcca aagggcggcg
     4261 gttcgggagg ctggtcatgc ggcgagcgat tctacgatgc ccattactca ttcagatcct
     4321 cgcgggagcc ggcccggccg gaaacggcag cccctgatcg accccggagt ggatgcgatg
     4381 atcaccagcg attcgcaggc actcccagta ttcttgcggc agaaagagtc gccacgccgg
     4441 gccgggacgc aaagctcggg ccggctcccg atttcgggtg gaactggcgc caggactgta
     4501 agaatccgtc cccagggttg ttcccccggt acgtcggttc cgcagtcttc cgtttgcagt
     4561 acaatggctc tgccacaggc tccgcaggag gactacatgg ccgccacgct cagcgccacg
     4621 agccgggacc tcgatcttca gcatctctgg cacggaaatt tgcagcaccg cgatctcgcc
     4681 aagcgcccgc cgctcgaaat cacgcgggcg gaaggctgtt atgtctacga tgccgccggc
     4741 cgccgctatc tggacgccat ggcgggtctt ttttgcgtca acgtcggcta cggccggcgc
     4801 gagatcgtgg ccgcggtcgc cgaccagatg tcaaggctgc cgtattaccc cctgacgcag
     4861 tcgcacggcc ccggcgcaag gctggcggcg cggctggccg ggcttctgcc aggcgaactg
     4921 ggtcgggtgt tcttcgtcaa tagcggctcg gaagcagtgg aaacggcgct gaaaatcgca
     4981 aggcagtacg gccggcaggc ccatcccggc cagaaccgtt acaaaatcat cgcccggaat
     5041 cgcggctatc acggcttcac gatgggcgcg gcctcggcta ccggacagat cttgcgcaag
     5101 caggcgttcg agcccctcgt gccaggattt ttccatgtct ccccgcccga ccactatcgc
     5161 tgcccgcact gctcccgcga gcctgcctgc accctggcct gcgcggatga ggtggagcag
     5221 gtcatccgcg cggagggtcc cgaaaccgtg gcggcggtga tagccgagcc tgtaatcggc
     5281 ggcgggggcg tttttccagc tcccgagggc tattgggagc ggctgcggga aatctgcgac
     5341 cgctacgaag cgctgctgat cgccgatgaa gtaatcaccg gctttgggcg taccgggaag
     5401 ctgttcggct tcgagcacgc cggcatcgtg ccggatatca tcactatcgc caagggaatc
     5461 acatccgcct atcttccgtt ggcggcggcg gtcgccaccg agcgcgtctt ccagatcttt
     5521 caagcgggcg agtcggatga agcgaagttt acacaagtca gcacgttcgg gggccatcca
     5581 gcctcctgcg cggcggcgct ggccaatctc gacatcctgg tgggcgaacg gctttgggaa
     5641 aacgccgcgc gcatgggaag ctatttgctc gaggcgctgc gcgcgattca ctcgccattt
     5701 atcggcgaag tgcgcggacg cgggctgctg atcggactgg aaatggtgga gagccaggat
     5761 acccgcacgc cgcttcccga ggcagcggcc gcaggccttc agcgcgcgat ccgcgatgcg
     5821 ggcgtcatcg tagggcgcaa cagcgacacc gtgcccgggt tctgcaacgt gttgatcctt
     5881 tcgccgccgc tgacgttgag ccgcgaacag gcggacgcga ttgtggaggc actggaagcc
     5941 ggactggctc aactgcactg aggccgcgcc atcttgcgcc gtcttcggca tccagccagg
     6001 ggcggccgtg cgctcagcct gcgcgttgct gttactgact gggtctgccg cttttttctg
     6061 cgcctgttag aatagttcta ctctgcgctc ccgaccctct gtggccaaga cgccattccg
     6121 aaaaatctta atcgccaatc gcggcgaggt cgcagttcgc gtgattcgcg catgccgtga
     6181 gatgggaatc gcttccgttg ctgtttattc ggaagcagac cgggccgcgc tgcatgtccg
     6241 catggccgat gaagcccgcg ctatcggccc tgcgccggcg ccggagagct atctgtcggt
     6301 ggaacgcatt atcgaagccg cgcgagccag cggagccggc gctattcatc ccggttatgg
     6361 tttcctgagc gaaaatccgg cattggcgcg ggcttgtgat gaggccggca tcgtgtttat
     6421 cggccccagc gccgggtcta tggaattgat ggggtccaaa acagcggcgc ggcagagaat
     6481 gctcgcggca ggcgttccgg ttgtgccggg cgccaccgct gcggccgctg actggcgcca
     6541 ggctcaggcg gaagctcgcc gctttggata tcccattttg gtgaaagcag cggccggcgg
     6601 cggcggcaag ggcatgcgag tagtggagcg cgaagaggaa ttacccgccg ccatgcgcga
     6661 ggcggccagc gaggcggagc gagccttcgg ggaccccagt gtttacatcg aaaaatacct
     6721 gagcaggccc cggcacatcg agatccaggt gctgggcgac cgctacggcg agctgattca
     6781 cctcggggaa cgagaatgct cgctgcaacg ccggcatcaa aaggtgatag aagaatgtcc
     6841 ttcgccgctg gtcgccgaac acgaagagat gcggcggaag atgggcgaga ccgccgtccg
     6901 ggcggcgcgg gcagccggtt actacaacgc cggcacgatc gagttcctgg tcgatgagga
     6961 cctcaatttc tatttcctcg agatgaacac ccggcttcag gtggaacacc cggtcacgga
     7021 gatggtgacc ggaatcgacc tggtgagaga gcaaattctg atcgcagcag gagaaccgct
     7081 cggacgccgc caggagcaga tcggctggag cggcgcggcg atcgagtgcc gcgtgtatgc
     7141 cgaagatccc agcaacaatt tcttcccttc gcccggccgc attgcgtcca tgagcgagcc
     7201 tgccggaccc ggcgttcgcg tcgattcggg cgcttacccg ggctggacgg ttcccattga
     7261 atatgatccg ctgtttgcga aactggcggt gtgggcccct tcgcgcgagg gggcgatcgc
     7321 ccggctgcgc cgcgcgctgg atgagtacca tgtcagcggc attcaaacca atctcggttt
     7381 cttccgagaa atcgtaaatg accccgaatt ccaggccgga cgccttgaca caggcttcat
     7441 cgcgagatgg atgtcccgcg cgaagacccc tccgctggaa tcctgcgacc aggaacgggc
     7501 ggtcgcttta gctgcggcgc tgtatgccct gcgccgggag aacagcgcag cttcggcctc
     7561 cgaagctgcg ccttcccgct ggaagactca gggccgcgcg cggctgctgc aatgaagctc
     7621 gagatcatca ttgacggcag ggcgcgggcg gtcgaagtgc cggaccaggc gctggccggc
     7681 ggcccgggca attttgtgat cgagtcttct cccattcagg gtgacatccg ggaaatcgag
     7741 ccgggcttat attccgtcct gatcgccggc cgcagctacg aagtaagaat cgatccggct
     7801 tccgcatgga gcgccggaac agagccggcc gcccgccctg ggacggacgg gccggcgccg
     7861 gattactacg cccacgtccg cggacggcga tatgcggtcc aggtgcgcga tccccggcgg
     7921 ctgccgctac agcgcggccg gccggccgca gccggccgcc agagcgtcgc ttccccgatg
     7981 ccgggaaaag tggttcgcct gctcgtgagc gagggggagc aggtgaaagc cggacagggc
     8041 ttgctggtcg tggaagcgat gaagatgcag aacgaaattc gcgcgcccaa agacggacga
     8101 gtaaccgcaa ttcgagtcgc cgagggcgcc gcggttgccg gcggcgagac gctggcggaa
     8161 gtggaatagg cgggcctgcg gccgggcggc cgcactgtca ggccttgacg ggaattggga
     8221 aggcgggaaa tgagtaccgg aggctggcaa aatgcgtgaa ttcgacgtcg taaccggcgc
     8281 gttcggatat acgggcaagt acatcaccgc gcgattgctg gcgatggggc gcgccgttcg
     8341 tacgctgacc gcccatccgg atcgcctgaa tccatttggc ggcaaggtca gcgtggcgcc
     8401 gttccatttt gaagtcgccg gaaagctggc ggcgagcttg cagggggctg acactcttta
     8461 caatacatac tgggtgcggt tctcgcacgg caaagtcact ttcgaccaag ccgtgcggaa
     8521 cacgcgaaag ctcatcgagg cggccggaac ggccggcgtc cggcggctgg tgcacatcag
     8581 catctccaac ccttcggaag attcccctct tccttatttc cggggcaagg cgatcttaga
     8641 acgggccatc gccgagtcgg gattgtccta cgcgatcgtc cggccgaccg tagttttcgg
     8701 ccgcgaggat attcttatca acaacatcgg ctggctgctc cggcgttttc cggtgtttct
     8761 gattcctggc tcgggccagt attgcctcca accggttttc gtcgaagacg tggccgacat
     8821 tgcgatcgcc gccgcctgcg cggagcagaa tggcgtgtgg gacgccgccg gccccgaggt
     8881 ttacgttttt gaggagcttg tgcgcgccat tgccgccgcc gtgcgcagcc gcgcgcggct
     8941 catcagggcc ccggcggggc tggtagcgct tctatgccgc ctgcttggat atttcgtcgg
     9001 ggacgtgctg cttacgcgcg atgaggtgaa gggactcatg gccggcctcc tggtgtccca
     9061 cgctgcgccg gcgggccgga aaaaactcag tgaatggctg gcggaaaact cggaaacagt
     9121 cggcgcggcc tacgcctcag agttgcagcg gcactatcgt tagctgaaga atgctgctca
     9181 cgccggccgc tgcaccctct cttctgagga cccgtctctg aggagcccgg cctcgcaccg
     9241 cgcgaagcaa tggacttcgg aggcgaccgg caacaagcct cgaaacagga accgggggcc
     9301 ggagccggaa cccggcatcc atccccggtt ccggaactga acctctgcgc ttcaaagctg
     9361 gactccggga ctgaatctaa aaaagcggat ctaggtcgag cctggataat ggggttcctt
     9421 ccggtttgtg cggaggaagt agtctagaat ggatcaatag gggcggttag ctcagttggt
     9481 tagagcgcct gccttacaag caggaggtca caagttcaag tcttgtaccg cccaccatct
     9541 attttcaagg acctatattc tcaagactta tgagggcatt agtgtaccct ctgctgagac
     9601 attgagatcc aactcgggga tccaacttcc ctccgccagg gcgctcctgc tgaccggagt
     9661 agatacgctc aatacgcccg gccgcgtccc gttgcttgcg tccaacatgc gcgcacaggc
     9721 tccccgggcg ggtgacggtt ctgtccggcg ctctgcttcg atcgccggaa agtggatcga
     9781 ttccgcaacg tcacgttgca gggcggcgaa cgtgcataat attgtcgagg gggcccaatg
     9841 agacacgcgg ttctatggac tgtaaatata ctgttgttat tcgtgctgga ggcggcggcc
     9901 cgaggacaga ccccgccgaa tacgctcagc gccgaagaaa tgcaggaggg atggattctc
     9961 ctttgggacg gcaaaacgct gaacggatgg gaatcccatg ggagcggcgt ctggaaggtg
    10021 gaggacggag ctctggtggg cgagccggag ggcaaagccg gctggctggg gacaatgcag
    10081 gcattcagcg atttcgtgtt gaaagccgaa ttccgcacca cggcggactg caacagcggg
    10141 atctaccttc gcgctgcacg tgagggcgtg ccgcgccaga ccggttacga gatccaaatc
    10201 cgcgactaca aggaaccgca agagaccgtg ccgttcctga ccggcagttt ggtgatgcac
    10261 gcgcacgctt cgggcgcaaa gatcatcccc aaccagtgga atcgctacga agtgaccgcg
    10321 cagggcgatc attttatcgt ggtctacaac ggtcaaacgg tgctcgatac ccgccagtcc
    10381 aaaacttcct ccggggtcat cggcctggaa tacaacaata gaaaaatcga gtttcgaaac
    10441 ctcaagttac gtccgttgcg ctgagaacgg gaaaaccacc cggcgccagg ggccggcgat
    10501 ccggctctag cgagtatgcc ggcgggccag ggtttttatc cagctccgct gcccggcaac
    10561 ttgggtggat cctgttggtc tcaggcgaaa ccggaacccg gagggacacg cgcctggaaa
    10621 cattcttata tgaaaacgtt ttgtctggcc gttttccagc gctcaaggaa ctagcgtgga
    10681 tcgtggcagg agccgcagcc caccgggccc ccccgcgtcc ggtggcagtc gatgcaggcg
    10741 gccattgcca gatcagtctc gcggcacgtc acgtcccgct cttgcaccag cccgtggcag
    10801 gtttgacatt cgacttttcc tttcttgacg tgctccgcgt ggctgaactc gacgtaatcc
    10861 ggcaggcgat acacaggctc ccagggaacc ggcttgtgct gcttgtcata ggcggccagc
    10921 cgatggatgg gtgtactctc ttttttgatc gtgatatggc atgccatgca ggttgcagtc
    10981 gagggaagac ccgcggaata gccgggcttt tccatggcgt ggcattctgt gcaacgcaag
    11041 cctgtcgcgg cgtgcttttt gtggctgaac gggaggggct gtggcggcgg cggggaaaaa
    11101 caatcctcgt cgccagccgc ggcggccgtc aggacggtga agatcgccaa gaggacagcg
    11161 tttcgagcca tggacccgga ggatgcagac ggcagacttt caggatcgca tcgatcggga
    11221 ggattgacaa ggataccggc gtcaaattga tccgctgttg acctcacgtt gctttcggct
    11281 ggctccacgc tgctggcgcg ccggccaaac gtcggttctc tatgcccaaa gaagcacgcc
    11341 acctggtcag tttcatcgcc acaccacagc ccgtggtgga aaagatgctg ggcctggcga
    11401 aagtggagaa agacgacgta gtctacgatc tgggctgcgg cgacggccgc gtggtaatcg
    11461 ccgctgccaa gaaatacggc gcccgtgcgg tcggcgtcga tatcaacccc gagcggatca
    11521 gggaaagcga ggaaaacgcc cgcctggcgg gcgtagcaga gcaggttatc ttccgcaacg
    11581 aggatctgtt tgaagctggg attgcggagg ccacggtggt cacgctttac ctggtggagt
    11641 cgctgaatcg gaagctccgc atgaagttgt ggcgggagct gcggccagga acgcgcgtcg
    11701 tatcgtacaa cttcgacatg ggcgactggg agccggaaaa ggaaatcgcg atcgaagggc
    11761 ggaagctata cctttggagg attcctttcc atccccctac ccggccctga tagccgcatc
    11821 gaactgtccc ctgaaaattt tggaaattac cgctcaagat cctgcccgtg agacatgcca
    11881 tcgatttccg gctctcaaag ccgccagagg gagtagaata tgagccatct agtgctcgtg
    11941 gaagtgagcg gaatgaatcg gatacacaac agcaagcatc tgctccacgc gccggcactg
    12001 ctggttttgg gcgttatcac agccggcgcc gccagcggcg aagtcagctt taaccgcgac
    12061 atccggccca ttatggccga cacctgcttc cggtgccatg gtcccgataa aaatgcacgc
    12121 atgatggggc tgcgcctgga catacgggag gaagccacgc ggcgcacggc gactggggtg
    12181 atccccatcg tgccgggcaa gcccgaggaa agcgccatca tccagcgcat tttcgcgaag
    12241 gatccggcgc ggcgcatgcc cccggtttac gcgcataaag agctgacaga gacgcagaaa
    12301 gaaacgatcc gccgctgggt ggcggagggc gccaagtatg agggccactg ggcgtaccag
    12361 cccgtgcggc gtcccgaagc gcctgcggtg acccaggccc tggcgccgat tcgaaatccg
    12421 atcgacgcct tcatccagtc gcgcctagct cgggaaggct tacgcccctc gccggaggcg
    12481 gaccggagaa cgctgctccg gcgggttacg ctcgatctga ccgggctgct tcctaccccc
    12541 gaggaaggga gcgcgtttct caaagatcag tcgccggacg cgtacgagaa agtcgttgac
    12601 cggctgctgg cgtcgccgca gtacgcggaa aagcaggcca tgcactggct ggacgcggtt
    12661 cgttatgccg atacggccgg gtttcacagc gatcttccct acccgatctg gccgtatcgc
    12721 gattacgtac tgcgggcctt cgccgagaac aagccgttcg accagtttac ccgtgagcag
    12781 cttgcgggcg atctgctgcc gaacgccact atcgagcaaa aagtcgcctc ggcttacaac
    12841 cggctgaacc ggatgtcgcc ggagggcggg ctgcaaccca aggaatatct cgcgaaatac
    12901 ggcgccgacc gcgtgcgcac gacgagcacc gtctggctgg gaagcacgct cggctgcgcc
    12961 gagtgccacg accacaaatt cgaccccttc ctctcgaaag atttctattc gatgaaggca
    13021 tttttcgccg acattctcga aacggggcgc gccccggaca tcggtcccgg cgcgtgggga
    13081 tccaagctcg atctgccgac gccggaacag cagaggcagc gcgagcaatg gagctggcag
    13141 cttgcccagg cgcggcgcga tctggacgcg gaagcccagg tgctcgccgc cgataccaac
    13201 tgggaagcgg aggtattgcg gcgctacgaa gcgggagaac tggattggcg ctaccagcgg
    13261 cctgtctccg cggaatcggc ccacggagcc aggctgacga tttacaacga tgagccggta
    13321 gaaagcaact actacatagg cggcacgctg gtttccgaac gcgccccggg ccgcggcctc
    13381 gttgtggtca gcgggccgaa ccctgacaac gaaacgtata cggtttcctt ccggccgggc
    13441 gagggcgcct ggactgctct gggcgtggaa gccgttcacg atgagagcct gcccagccac
    13501 cggctggcgc gggggtccga ccgcctggtg ctgaccgagg tggaggctca agtttcggag
    13561 ggcgccagcg gaccggtccg aaaactgtcg tttgtgctgg ccaccccgag catgcgaatc
    13621 gagccgcctg agctgcaccc gatggcggtc atcgacggag atccccgcac cgggtggggc
    13681 gtggcctcga acggcgaccc gggcaatttg tttcttgcgc tgcggttcgc gcagaagctg
    13741 catacgaccg cggattcggt ggttaccgtc cggttgcggc acgattccca gttccggcgc
    13801 gccacgctgg gtcgatttcg gctcgcgctt tccgccggag agtattcctg gccggagccg
    13861 gagaatttgc gagcccgctt gaagaaggag accggagacc cgccgccgtc aaaccatggc
    13921 ggcgtgccgg cccgggtgct tacagccttg cggactccgg aagacaaacg cacggaggag
    13981 cagaagcgga cgctgttgga ctattacgaa tggctcacgc cggacttgga acatcgcctg
    14041 gcgcagatcg gcaaactgga agcgaaactg gggcaattgg agtcgcagat tccccgcgtc
    14101 gtcgtgaccg aagcgacacg gccgcgggtg acccgcattc tgccgcgcgc caactggatg
    14161 gatgacacgc atgaaatcgt cgagccagcg attccggtgt ttctgggcaa actggagacc
    14221 ggcggccgcc gggccacgcg gctcgatctg gcgaactggc tggtctcgcc gcgcaatccg
    14281 ctcacggccc gcgtgttcgt caaccgattg tggcgcgagt ttttcggcgc cgggatctcc
    14341 aagacgctgg atgacctcgg gtcgcaggga gaatggccgg tccatccgga gctgctcgac
    14401 tggctggcgt cggaattcat gcagccggcc tggcaggcgc agggcgcgca cccgtgggac
    14461 gtgaagcaca tcatccgcac tatagtaact agtcatacgt atagacaatc ctcgctgccc
    14521 aggccggagg tggaagagcg cgatccggac aatcgtttgc tggcgcgcca gagccggttc
    14581 cgggtggacg cggagatcgt gcacgatatc gcgctcgagg tttcgggcct gctggtggaa
    14641 aaattcggcg gcccgagcgt gcggccgtat cagcccgatg gctacctggc tgcattgaac
    14701 ttccccaagc gcgattacag cgccagccgc ggagaggacc tgtaccggcg cggagtctac
    14761 attttctggc agaggacctt tttgcatccg agcctgctgg cgttcgacgc ccccacgcgc
    14821 gaggaatgca cggtcaaccg cgtggcttcg aatacgccgc tccaagcgct ggatctgctg
    14881 aacgacccga tatacgtgga agcggcgcgc gctttggcgc aaaacatgat ccagaacggc
    14941 gggcgcacga cggagacacg cattcaatgg gccttcgagc gagccctgac gcgtcccccc
    15001 cgggcggagg agcagcgcat tcttgccgat ttgtacacaa agaatctccg gcgcttccag
    15061 gctgcgccca tggaagcgaa aaaattgatc gcaaccggcg aaacgccggc gccgcagcgg
    15121 attgagccgg cagagctggc ggctatgacg accgtggctc gcgcgattct caatctgcac
    15181 gaaacgatca ccagaaatta ggaggaagct gtggatcgaa gagatctgtt tacggacatc
    15241 cgccgccgga cgtttctgag ccgcggcgtc gctgggctgg ggctgctggc gctaaactcg
    15301 ctgctcgacc cccggctgtt cgccgcagcc tcggacttgc aatccaaagg ggccgtaaat
    15361 ccgctgcatc acccggccaa ggcaaaacgg gtaatcttcc tttaccaggc cggcgggcct
    15421 tcgcatctcg agaccttcga ctacaagccc aagctggcgg aaatgaacgg caagcccatg
    15481 cccgaatcgc tgaccaaagg gcagcaaatc gcgcagttgc agggcaagcc cttgatctgc
    15541 ttcggcccgc agcacaagtt tcagaagttc gggaagtccc agcaggagat ttgctctttg
    15601 tttccgcaca tcggcagcgt ggctgacgat atttgcatcg tgcgctcgat gtggacggag
    15661 cagatcaacc acgacccggc gcacacgatc atgaatacgg gttcgatcct gccggggagg
    15721 ccgagcatgg gatcgtggat tttttacgga ctcggatccg aggcggaaga tctgcccgga
    15781 ttcgtggtgt tgacctcggc gggcaggggc ggccagatgc agcccatcgc cgcgcggcaa
    15841 tggtcggcag gattgctccc cggcaagttc caaggcgtga agctgaactc ggtgggcgac
    15901 ccggtgcttt acattgagaa cccggccgga gtgagcgcgg aggtgcagcg cgactcgatc
    15961 gaagcggtca acaaactcaa ccggctggaa tacgactcgg tgaaagatcc ggagattctg
    16021 actcgcgtgg cgcagtatga aatggcgttc cgtatgcaaa ccagcgtgcc gagcctggtg
    16081 gatatgagcc aggaacccaa acagattctc gagatgtacg gcgctcatcc cggagacggg
    16141 tccttcgctt ccaattgcct gctggcgcgg aggctggccg agcggggagt gcggtttatc
    16201 cagctctatc accgggattg ggaccaccac gacgccgtca aacagaacgt agcgctgaaa
    16261 gccgaagagg tggaccggcc aacggccgcg ctgatccagg acctgaagca gcgcggcatg
    16321 ctcgacgaca ccctggtagt ctggggcgga gagttcggcc gcacgccgat gtcgcagggc
    16381 ggcgatggcc gcgatcatca catcaaaggt ttttcgttcc tgctggcggg cgggggcatc
    16441 caaggcgggc ttacctacgg atcgaccgac gacctcggct atgcggcgca ggagaatccg
    16501 gtcagcgtgc atgactttca tgcgaccatg ctgtatcttc tgggaatcga tcacgagcgg
    16561 ctcacgataa agtttcaggg catggacgcc cgcctgacag gggtgtcggg ccgcgtggtc
    16621 aaagaaatct tggcgtaaag tttcccttcc ccagcggact gcacacagcc tcccaccagg
    16681 ctcggcggct gcgcgataat cgccgcatgg ccactctccg actcattgaa tacgccgatg
    16741 ccagccccga agtgcgggag gtctacgacg acatcatggc cacccgcaag acggactgga
    16801 tcaacaattt ttggaaagct ctcgcgcgag atcccgtcac cctccgaaga acatggcaga
    16861 gcatcaagga agtgatggct cccggcgcgc tggacgcgct cacgaaagag atgatctacc
    16921 tggctgtcag cgcgaccaac cagtgcgcgt actgcatcgc ttcccacacg gccggcgcgc
    16981 gcaaggccgg catgacggac cagatgttcg ccgaactgat ggccgtgacg ggcatggcga
    17041 acgaaacgaa ccgcctggcc agcggctacc aggttgagat cgacgaaaaa ttcaagaacc
    17101 cgtgaagacg gcttagcaag agccttgcgg tgcggcatcc ggcgctctcc acacacgcgg
    17161 aagccgggcg cccaagcgcg gatccctcgg actgggggaa tccctcgcgc cgggtttcag
    17221 attcgtgaat cacaccatct cctgatgcgg cgattggcgg gctgctcgag catccggaaa
    17281 acggcggcgg agatgatcac caccgcgggc acatagactg cgggcgtcgg ctcggcggcg
    17341 aggcgccggt accaccaaag cagaggaatg tggaggatat acatcgagta gctggtttgc
    17401 ccgagaaaaa ccatgattcg tgaggaaagc catcgtgcga gcacgccgcc ttccagagaa
    17461 agtccgatga gaagcagagc gttgaggggc cgcagagcgc cgttcagatc aatgggccca
    17521 cggatggtct cgggattgag aataaaaaac gcgctcagga ggatggccgg aacatagagc
    17581 cagtggccgc gtccggtgaa ggggcggcga gagcggagca gaagctcgta ggcgcgcgcc
    17641 gtgagaatgc ccatgataaa gtcggcaagg tgaatcaccg gtttccagac gtcaggcacg
    17701 cccagacgca ggagcgcgac gggcaggaaa caaacggcgg cgccgatggc cagtgcgttg
    17761 cgccagctta tcttagacag acataccgcg cccagcggaa aacataaata gaagaaaatt
    17821 tcgcaggaaa gagaccacgc gggagtgttc cagtggacag gcggggtccc ggcccaccct
    17881 tgcaataaca ggccatagcg cgccacaagt cccgcctttc ccggcacgcg gtcctgtatg
    17941 atgaacggcg cgacaatcaa cagactcaga gcatagccgg gataaatgcg ggcgatccgg
    18001 ccggcgccat agcgcaaaaa gctgccccgg ttccagacca tcgagccata gccgcgggcc
    18061 agcacgaagc ctgagaggac aaagaagatt ccgaccgcca ggtagctgct gtgaagaatt
    18121 acgtacatcg gatgcggcag ccgaagagac cacgcgtcca gttccgtccc cgtgtccatc
    18181 aagtgattca ggattaccca gagcgcaaga aagaaacgca agcccgtcag ggccggcaga
    18241 taccgtctct tcgccgcgag atagggagtt tcgtcggccc ttggacgctc tgctggcgcg
    18301 gatggggtct caaggaccgc ctcttgagca agacccataa gcaatgcgct ccttgattgt
    18361 atcgagccgg agagattttg cgccactccg ggaagcggcg gttcgcgccg cggttccggc
    18421 ggcgcaacac ggtatttata ataggattgg gcgaccggcg cgtttcaagc gtgccgcaag
    18481 gcgggcgcgc agaatgcccg cgctcgcgcg tgagatcaga ttctttgcag gcttacgcgc
    18541 aggtcgatgc gtgcgccttc gccggggccg gttttcacca ccagcgcgcc gttcaccagg
    18601 cgcacgcgtt cggccatgct gatgaggccg agcccctgcc ggctcttatc tcccgggtcg
    18661 aagcctttcc ccgaatccgc gaccgtgagg cgcagcgatc ctccggcgga ccgcagcgag
    18721 atcacgatca tttgcgcccc cgaatgcttc gcggcattct ggatggcctc ctgggcgacg
    18781 cggtaaaaac ataaagccac gtcctgggga atcgaatccg gcaggccacg ctgccgaaac
    18841 cggatcttga tgcctgtcct cttttgcact tcttcacagt aggaccgcag cgccacgccc
    18901 agtccgaggt gttccaaaat cgagggatgc agttggtagg ccgtgcggcg cacgtcgtcg
    18961 gagatttctt ccgcccgccg ccggagagat tgcagttctt tccggatggc ggccgcggaa
    19021 tcgggcaact cctgctccag ttgctcgacg cccacggcca gcatgcagag cttctgattc
    19081 aagctgtcat gcagctcccg ggaaaggcgc cgccgttctt cttcctgagc cgtaatcagc
    19141 gcggcagtca gggcgcccag ccgctcatcg cggtggcgta attcttccgc gtggcggaga
    19201 tcggtgatgt cgcaaagaat ccacacggct cccgttatcc cattgccgcc cgctatggga
    19261 tgtgcgctcg cgcgaaacca gcggtcgcgg ataagctggc ggctcgtctc gtgggctttg
    19321 gtgcgcaggg ctcgggtcag cgggttctgc tggcaggccg gcactatatc ttgataatgc
    19381 cttcccggaa ggacgccggc ggcctccccg aagatgagct tgaatttttc gttgcagcgc
    19441 cgaattttcc cgccagcatc gaccagggcg acggcgtcat gaacgctgtt gaaggtcgct
    19501 tcccagtcgc acgccatcga ctgcgctttc tcttccgcct cgcgcgcccg cagcagcgcc
    19561 ttgagaatcg ccgtcaacgc ggtatgatcc accggcgcga ccagataggc gtcggcgccc
    19621 ccttcggcgc cggcggctgg aacagcagcg cacgcagcag agacgtgcag aacagctatg
    19681 gaagccgtct ccggatcagc cttgatctgc cggcatacct ggaccccgct tatgtccggc
    19741 agatttgcgt ccagaatgac ggcttccggc cggtcggttt tcgcttgggc gagcgcctcg
    19801 gttcccgtag ccgcttctcg cacctgaaag cccgcctgcc gcaatgcgca gctcgtcaca
    19861 tagcgggagg cttgcgtatc gtctacgacc agaaccacag gctccacgcc gccgctcata
    19921 ggatcaccgc tccggcggcc atcccttccc cgccggccac ggcaagccgt attttcgatg
    19981 tattcacaaa catttcagac ttccagcttc tgttttttgg acgcaaccgg aggagagaga
    20041 gtcggagcgg cagacgccag tatagcatcc acagaattca gcgccaccta gggagaaccc
    20101 gagccgccgg gcaaaaaggg gcatgggccg gcgcacgggt ttcagcggtt ccccgccggc
    20161 cgggggagga agacaaaagc gcgtgggact ttcccgccct tcggccgctt caaatctttc
    20221 cccgggaacg ttcttcgatt tcgcgggtaa tgtattccgt gtaggcgatc agcgccgcgc
    20281 ggtacttccg gttggcggag aagaattcat cgttagcgcg aagcagagcc gcggtgctgt
    20341 cgggagtccc agccagatcg cgagaaagcg cggtggctga ttccagccgg tccagggcgg
    20401 cgcggtgagc gagccgagcc ttttccagct cttccgagag cttttctcgc gacataatct
    20461 cgccgctccg gaattagaat agcacaggct gcggctccct attgctgtat cttgcgcgga
    20521 aaagaaatgg tgaaccgcgt tccctggctc cgatccagtt gcagtgttcc ctgcaattgt
    20581 tcgaccagca accttaccag ttgcagcccc atagaggtgg cgtgggccgg atcgagatta
    20641 gcgggcaatc cgacgccgtt gtcggccacc gaaagaacgc actgcgagtc tccgcactcc
    20701 agagtgacct ggaccaaccc ggagcgttcc ttcggaaacg cgtgcttgag gcaattgctg
    20761 agcagctcat tcacaatgag gccgacggga accgcctggt tgagttcgag ctcggtgttc
    20821 cgcatgtctg tgcgcagccg gatgcggtcg ggatgggccc ggtagaattc gaacaggtct
    20881 ctcgccagct tttggatgta cccgccgaaa tcgatgtggg cgagatcagg cgatccgtac
    20941 agcagttcat ggatggcggc gatggactgc acgcggtgat gcatctcttg aaacatggct
    21001 gcggcttccg gatcgcgcag gtaatcggac tgtagctgga tgaggctcac aatcatttgc
    21061 agattgttct tcacccggtg atgaatctct tttagcagga cgtctttttc ctgaagagac
    21121 cggcgcagcc gctcttccgc ctgcctctgc tgggtagtgt cgcgcatcac catggcgaaa
    21181 ccgcgcagct tgtcggtctc gtcccggaca ctggtcagga tggcattggc caaaaaccgg
    21241 ctgccatcct tccgggaaaa ccagcgttcg tcctcggcgc tcttttgggt ccgggctttt
    21301 tcgagcagcc gctccggttc gccccgcccg gtgtcttcgc gtgtgaacag gcgggagagc
    21361 ggctggccca gaatttccgt ttcggtgtag cccaaaatgc gctcagcgcc ggcgttccaa
    21421 ctcgtgatat tgccgctggg gtccatttgg aacatggcgt agtcgcgaac gctctcgaca
    21481 aacagccgga agctgcgctg gctctccctg agggcggcgc ggtcggtggt atcgcggaca
    21541 ttcatttgaa tcacctggcg tgttccgacc agatagcggt tcgccgtgat ttgcagctcc
    21601 agccggcggc catcgcgggc gatgagcggt acgtcatcgt accggacgac gtctttttcc
    21661 atcagctcct gcataagctg ccggctgaac ggcgcgtccc ggaagggagc cgtctggttc
    21721 aggcggcgcc ctacgaactc ttcccgggga tagcccgtca gttcgagcat gtagggattg
    21781 atgtccatga tggacccggc ttccacatcg atcaaaagaa ttccgtcctt ggaggcctca
    21841 aacagccggc cgtagcggat ttcagcttcc tggcggcgct tggtgacatc ctcgatcgcg
    21901 agcagcacgg tgcgcgcttc caggcgggga atgcggcggg cgctgaggta catggtgcgg
    21961 cggccgatat ggggaaaatc gtgctccact tcgtaatcct ggaaggggat gccccggaac
    22021 agggcgtccc ccagcagctc ccgcaggcgc tggcggttcc actggccatt gccgaggtca
    22081 tagagcaggc gcccttcggt ttcctcgcgc gacacctgaa acatttcgta aaaagccaga
    22141 gtggctcgct gcacccgcaa atcgccgtcc agcacgagaa ggggctcgcg gacggtttcg
    22201 atcatgcctt cggcgtaatc tctggcttcc tcggccgcgc ggaggctgcg cttgatgggg
    22261 tcgatgtcga caatggtgat gacagcgccc tcgattcggt cgtcggtggt ccggtagggg
    22321 cgcaccgtga ctgagtgcca gcgcccgttc tggtcctgga catcctgctg ttccggcttg
    22381 agggtttcca gcacgccgac aatcaacttc tcaagaggaa gcggttcgag cggcccccgc
    22441 aggtgcccga tgggccggcc cacatcggcc gggcccagct cgagcagctt ttccgccggc
    22501 gtgttgaagc ggcggaggcg aaggctgtta tccaccatga cgatgggaat gctcaccgcc
    22561 gaaaacagat tcttcaaatc gctattgagg gccgcgagct cctggttgcg gtgctggagc
    22621 tcctcgttta cggtcgtaag ctcctcattg gcgctttgca attcctcttt ggtagtgctc
    22681 agctcctcat tggtgctctg gagctcctcg ttggcgctgc gcacctcttc gttggcggcg
    22741 cgcagctcct cggcgtgggc ttcatgatct tcggtgagat tgcgcaggta ttccctggtt
    22801 tcggcaagct gctgctgcaa ttgccgcgcg tgctcttcga gcgcctcggt gtagtcctcg
    22861 aggctgctgc ttctctgtcc aacgttgaat tggagcggag gcgcggcgct tttcttggcg
    22921 ccttcctgaa atacgatcag ataataccgc tcgccgggtg cgactccggc aatcggcgtc
    22981 acttcgatgt tgacctgacg ctgctcgccg ctatgatcca cataaatgcc ggtctcggac
    23041 atcgaaacgt tctgctcggc cacggcctgg atgaggcgcc gcagcacaag gacgagaccc
    23101 tcccgcgcca tccgcagcag attgaggctc gcttcgcccg gcgtcggctc caggtattcg
    23161 ctggtgtgtc cccgaaactg
//
LOCUS       DASREK010000094        13270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_723922, whole genome shotgun sequence.
ACCESSION   DASREK010000094 DASREK010000000
VERSION     DASREK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13270
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723922"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFA54_04900"
     CDS             <1..197
                     /locus_tag="VFA54_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50176.1"
                     /translation="ARSENELRALLFGALQNPARLAARRAALIREMFGCTLDGFSSRR
                     VAEQLGKLAAASHDCCSSSA"
     gene            264..1319
                     /locus_tag="VFA54_04905"
     CDS             264..1319
                     /locus_tag="VFA54_04905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS50177.1"
                     /translation="MASEPAKIRPGKNARSISVVTPTYYRRDEIDQLLDSLSGQTVRP
                     LELIIVDGAPVSEQATERLVASRSSALPFRCRYIRQSGGTAIQRNAGIDIAQGDFVSF
                     IDDDISLEPDFFEQILLAYELDKDQRVGGIAGYITNQHLDPNKSPRWRWYRRLHLFTT
                     YEPGRYDYRTGYPINRYLQPPHAGIRTIDFMGSNCAVWRREVFASGLRFSPFFSDYGV
                     LEDAHLALRAGRDWTLWECGLARCVHHRSPKGRVSKRRLAWKTAVNYRYVFVDIVPQR
                     NWKQELRFWSVQAVDLLRLCAHAFRSREKGDWPAVLGKVEGILAAMRLKKAGPAVPIG
                     STSADSAPVSEGTICSS"
     gene            1316..2482
                     /locus_tag="VFA54_04910"
     CDS             1316..2482
                     /locus_tag="VFA54_04910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS50178.1"
                     /translation="MKCAVFSHKPCWRSRASPSGYATDGGFAFQMAALAEMFDETTVA
                     VPIYKCGPPRGEIALAGRNLKIAPLVPPLGRGVWRKALMPAWFLWNLPALLREMSRAD
                     AVHVPIPGDIGTIGMVLALVLGKPLFVRHCGNWFVERTAAERFWKWFMVRFAGGRRVM
                     LATGGAEEPPSPRNSHIGWIFSTSLTEAEIRACGQRRSSIDPQRLRLIIVCRQEKEKG
                     TGIVIDGLRLVCQDFPGASLDVVGDGSALPQFRKQAADLGLAERITFHGRLGHGEVIS
                     LLQQASLFCYPTAASEGFPKSVLEALACGLPAITTRVSVLPQLISQGCGILLDAPTPE
                     AVATAIRRCVDHQDEYRFMSARALEIAREYSLEKWRENIACLLRAAWGPLACDA"
     gene            2472..3566
                     /locus_tag="VFA54_04915"
     CDS             2472..3566
                     /locus_tag="VFA54_04915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS50179.1"
                     /translation="MTRDLARLKICFLAGTLGQGGAERQLIHILKALRCHGAGVRLLC
                     LTQGEFWEEAIRKMEIPVIWVGRQKSRLARLGGILRVLKNDPPDIIQSHHFYTNLYAT
                     AAARIIGSRDVGALRNDPGGEMKFHGFVTGQLSLRAPRLLVSNSRAAMDRASALGIPS
                     SRLRWLPNVVDLDRFCPGGCEHNGRVRLLSVGRLEEQKRHDRLLRALKKQLSGPCGRD
                     FRLVIAGEGSERPRLEALTRGLDLLPRVQFRGGVVETDALYRESDIFVLASDWEGTPN
                     VILEAMASGLPVVATRVGGVPEIVQHGETGYLVDPDDESSLAEALSILIGDCRLRREM
                     GRRARKYVEDNHSPQRLGRLLQELYEAALS"
     gene            3584..5056
                     /locus_tag="VFA54_04920"
     CDS             3584..5056
                     /locus_tag="VFA54_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS50180.1"
                     /translation="MTANTVQTWRVPLLQKTSLPIRKLGLFALAHVPLGLLMHQFNAI
                     ATVHAIVTFLIGCGWAIYGRRPERVAYVAAYITGAEVLWRMASADVFWEFGKYAVAGI
                     FLLALLRTGKLQHFALPLLYLGLLTPSTVQTIQDLRWAEARSQISFNLSGPFALAMSC
                     WFFANVRLSLKQLQRLFLVLIAPIAGIATIALYGTVAASEIHFTTQSNFETSGGFGPN
                     QVSAVLGLGALLTFLILLTYQGKKAGVKLCLFCGLALFAIQSALTFSRGGLYNAAGAG
                     AVASMLFLSKTKFKLRVLAVAVLVLIIAHYVVLPSLDDYTGGALSERFSSLNLTGRDR
                     LIRGDLQAWEENPVFGVGPGRARFSRGEFYREGVAAHTEFSRLLAEHGMWGLAALFTL
                     LFMAIKNFRQARSGVHKALLTAATAWSLLFMTNAAMRLVAPSFVFGLASVKLLRQRKI
                     LQWTTGPYPSHQGQVAVKQIGAVNPAFTQRAPGRLSSAYE"
     gene            5193..6299
                     /locus_tag="VFA54_04925"
     CDS             5193..6299
                     /locus_tag="VFA54_04925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZS50181.1"
                     /translation="MRIFAVGDTAFSGRVAASLEGRSSPIAGGVAALLQSGDIVFGNL
                     EGSLSDSIPRGSLFAAPASAASLLSDSGFTLLHLANNHAYDYGPAGLGSTLAALASAG
                     VTPLGAGTDSFSARRPVRTDRRGLRIGWLGCGRSECAPASDGPQFWEFDEAELLQAVT
                     SARREVDFLVASLHMGFMYVDYPHPDHRTLARRLAQAGADLVLMHHPHVLQGIEITPE
                     GRGICYSLGNFLLDWREGNVAPEVMIAEQNQGAILLFDVDREGVSLMAALPTRIEARS
                     TVQWATGEAGRKILRRLARLSDDLKGDCTASFWKQRSERNTVLAVRVMWFHLSRGHWA
                     VVWNGLKRVRREHLTMLARWIRQFFRHPAETAAP"
     gene            6338..7363
                     /locus_tag="VFA54_04930"
     CDS             6338..7363
                     /locus_tag="VFA54_04930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS50182.1"
                     /translation="MIGNFLSGSAGTRGVCEDLADRLISAGWPVLFASSKPGKLRRLW
                     DMLSTARAQRDRYDVAHVDVFSGPAFFWAELVCWILQRSRKPYVLTLHGGNLPEFARR
                     WPRRVRRLLGSATAATAPSGYLREQLRAYRKDLQLLPNPVDVASYPFRLRERPTPSLI
                     WLRAFHEIYNPSLAVQTLALLTTDFPDAQLTMIGPDKGDRSLQSAQRLAGKLGLDRQI
                     TFVPGVPKTEVPRRLLEADIFLNTANIDNTPISLIEAMASGMCIVTTSVGGIPYLLDH
                     EKDALLAQPGEAESMAHGVRRILTEAGLAGKLSRNARRKAQQFDWRLIFPRWEALLRS
                     VADKPAA"
     gene            7360..8799
                     /locus_tag="VFA54_04935"
     CDS             7360..8799
                     /locus_tag="VFA54_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZS50183.1"
                     /translation="MSNAWIRIYHRLPQPCRSAAAAVWGFRLRFWRYGPESEQLVTEA
                     LQRDYWQPQQWEKWRAERLGYVLHRAATRVPYYREQWEMRRRRGDRASWDRLENWPVL
                     KKEMVRANPRAFVADDCDPRRMFHDHTSGTTGSPLSVWLNRNTVRAWYALFEARCRRW
                     YGVSRFDRWGILGGRLVTPADQRKPPFWVWNPALNQLYLSSYHLAPDLTPYYLEALAR
                     YRIQYLLGYTSSLCALAQAALRTGRRSLPLRVIVTNAEPLLPHQRQMISEAFQCPVRE
                     TYGMTEIVAAATECEWGRLHLWPEAGRVEILEHDNPVQPGACGDIVATGLLNADMPLI
                     RYTTGDRGALAANNIPCSCGRLLPVLASVDGRADDLLYAADGRQIGRMDPVFKSDLAV
                     REAQIIQETLGTVRVRYVPAAGFTDRSANTIIEAIRSRLGPVEVLLESVPQLPREPNG
                     KFRAVICRIPPEQRPDGQRCGSELTSTLV"
     gene            8796..9908
                     /locus_tag="VFA54_04940"
     CDS             8796..9908
                     /locus_tag="VFA54_04940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS50184.1"
                     /translation="MTRNADLSAQTRVSGSIRNPQPIVSIIVPCRNEVKHIRGFLDSV
                     FEQDLAGFDWEVIVADGMSDDGTWPLLEEYARKQPRLRLLRNPAGIVSTGLNACIRAA
                     RGDIIVRMDAHTEYAPNYVRECLRALEETGAGNVGGPARTRADGLWQRAIAAAYHSPF
                     ACGGVRFHNDYYEGFADTVPYGCWKKFTLVELGLFDETLVRNQDDELNLRLTRSGGKV
                     WQTPRIVSYYHPRTELSKLFLQYFQYGFWKVAVIRKHRLPASWRHLVPGAFVAALGIS
                     FLSWLISEGGGRLGLAAVSESLLLTLLLSYAGCCVIAAFTSARSHGWSIFPILPPIFA
                     VYHFSYGLGFLCGLACALFPGSRLSRPARAFVEISR"
     gene            9976..10692
                     /locus_tag="VFA54_04945"
     CDS             9976..10692
                     /locus_tag="VFA54_04945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50185.1"
                     /translation="MPSAYPTGSEIERIRAEYARRGREIPQDFYSWSRPVNQFFHCQT
                     LRACVAALVREALFPLTGKTIVDIGCGSGEWLTEFIQWGARCADVGGIDLRPDKIEEA
                     RKRLPEANLHTGDARRLPFPSNSADLVSQFTVFTSILEPHFKKAVAAEMLRVLKPGGL
                     ILWYDFRFNNPSNSSVRGIKSEEIRTLFPGCRIHLRRVTLAPPVARFVVPITWIGALL
                     LEKIPILRTHYLGAIRKTAI"
     gene            10738..11379
                     /locus_tag="VFA54_04950"
     CDS             10738..11379
                     /locus_tag="VFA54_04950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZS50186.1"
                     /translation="MTKVLLDRMCAAAALVLLLPLWFFIAVAIAWDDGLPILFRQTRI
                     GRGNVPFQLLKFRSMRPRVPGPRITAGNDPRLTRVGAFLRRFKLDELPQLWNVLKGDM
                     SLVGPRPEVPAFVDFQDPTWQTVLQVRPGITDLASLVYRNEEEILCGQNDPESYYRNE
                     ILPAKLALNMHYLRSRSLWSDLKIILITMRYSFFPAGFHSSSISRMFLKQENS"
     gene            11376..12569
                     /locus_tag="VFA54_04955"
     CDS             11376..12569
                     /locus_tag="VFA54_04955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZS50187.1"
                     /translation="MSEQSFIPFHIPSIGEEEISEAAAALRSGWLTTGPRTAQFEREF
                     KEYVHVSHALAVNSGTAALHLALAGLGIGPGDEVITTPLTFCATVNTILQVGAVPVLA
                     DIGADGNIDPASVAQRITGRTRAILPVHLGGLPCEMDQLWSLARQHGLFVIEDAAHAV
                     GSLYRGQPIGGGSDAVAFSFYATKNLTTGEGGMVTTNNSALAESMRILCLHGITKDAW
                     NRYAQHGNWYYEVQACGFKYNLSDIQSAIGIHQLRKQEHFIEVRTRYARLYSEALAGV
                     PEVDIPPDPEWRRHSWHLYILRLNLELLDINRDEFIRELHRKGIGASVHFIPVPLHPF
                     FKEYASRPENFCPRALALYPRLVSLPLYPAMSENQVSYVSRTVKEIVTAAKRRKPVFL
                     AATTP"
     gene            12566..>13270
                     /locus_tag="VFA54_04960"
     CDS             12566..>13270
                     /locus_tag="VFA54_04960"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50188.1"
                     /translation="MNTKIIKKLFYRFRRLFIALWHLAIITAALLSAFLLRFDFSIPA
                     SEVPLLFRVFCIAVVSKMLAFYLAGIDRGWWRFVGMADLIRVLVANAMGSWALALMAS
                     FLVGSRFPASIYFIDFLICFLATAGGRFCVRLYYETIAGELSRPGRRRLLIYGAGMAG
                     ATVLKEIRANQSLGYDVVGFLDDDPKKRNGRLLGVPVLGAGRDASRIIDHYKRNNRNI
                     EEILIAMPTATGRQMQE"
BASE COUNT         2417 a         3948 c         4041 g         2864 t
ORIGIN      
        1 tggcgcggtc cgaaaacgaa ctccgcgcgc tgcttttcgg cgcccttcaa aatcccgcac
       61 ggctggccgc ccgcagggca gcgctgatcc gggaaatgtt cggttgcact ctcgacggct
      121 tttccagccg ccgggtagcg gagcagctag ggaagctggc ggccgcgtca catgattgtt
      181 gttcttcatc ggcatgattg attcgcgctg gggattcagc ccagggcgct ccaatccccg
      241 cctaccctcg caaagcgttc catatggcat ctgagccagc caaaatacgc cccggcaaaa
      301 acgctcgctc catctcggtg gtcacgccga cgtactaccg ccgggacgaa attgatcagt
      361 tgctggacag cctgtctggc cagactgtgc gccctctgga gctgatcatc gtggacgggg
      421 ctccggtgag cgagcaggcc acggaaaggc tggtggccag ccggtcctcc gctttgccgt
      481 tccggtgccg atacatccgc cagagcggcg gcacggccat tcaacgaaat gcggggattg
      541 atatcgccca gggagatttc gtctcgttta tcgatgacga tatatctctc gagcctgatt
      601 tcttcgagca gattctgctg gcttacgagc tggataaaga tcagcgcgtc ggcggcatag
      661 ccggttacat caccaaccag cacctcgacc cgaataaatc gccgcgctgg cgctggtacc
      721 ggcgcctgca cctcttcact acctacgaac cgggacgcta tgattatcgg accggttatc
      781 cgatcaaccg ttatttgcag ccgccgcacg ccggaatccg cacgatcgat tttatgggtt
      841 ccaattgcgc cgtctggcgc cgggaggttt ttgcaagcgg actgcgcttt tcgccctttt
      901 tttccgatta cggcgttttg gaagacgcac acctggcctt gcgggcgggc cgtgactgga
      961 cgctttggga atgcgggcta gcccgctgtg tgcatcatcg ctcccccaag ggccgggtca
     1021 gcaagagaag gcttgcctgg aaaacagccg taaactaccg gtacgtgttt gtggacatcg
     1081 ttcctcagcg aaactggaag caagagcttc gtttttggag cgttcaggca gtggatctgc
     1141 tgcgtctttg cgcccacgct ttccggtcgc gcgagaaggg cgattggcca gcggtattag
     1201 gaaaagtcga gggcattttg gccgccatgc ggttgaaaaa agctggcccg gccgtcccca
     1261 ttggttccac ttccgctgat tccgcccctg tttcagaagg aaccatctgt tcctcatgaa
     1321 gtgcgccgtg ttttcccaca agccatgctg gcgcagccgc gcgtcgccca gcggatacgc
     1381 cactgacggc ggctttgcgt ttcagatggc cgcgcttgcg gaaatgtttg acgagaccac
     1441 cgtagccgtc cccatttaca agtgcgggcc gcccagaggc gagatcgctt tagccgggcg
     1501 caacctgaaa atcgcgccgc tggttccgcc gctgggacgc ggtgtgtggc gaaaggctct
     1561 catgccggcg tggtttcttt ggaatctgcc ggcgctgctt cgcgaaatgt cgcgcgccga
     1621 cgccgtgcac gtgcctatcc ccggcgatat cggcaccatc ggaatggtgc tggccttggt
     1681 cctcggcaag cctcttttcg tgcggcattg cggcaactgg ttcgtcgaaa gaaccgccgc
     1741 cgagcgcttc tggaagtggt tcatggtccg gttcgccgga ggccggcggg tgatgctcgc
     1801 cacaggggga gccgaggagc cgccctcgcc gcggaattcc cacatcgggt ggattttctc
     1861 aacgtcgctg acggaagccg aaatccgggc ctgcggccag cggcgcagct ccattgatcc
     1921 ccagcgcctg cggttgatca tcgtgtgccg gcaagaaaag gaaaagggca caggcattgt
     1981 tatagacggt ctgcggctgg tctgccagga ttttcccgga gccagcctgg acgtcgtggg
     2041 ggacgggtcc gcgcttccgc aatttcggaa gcaggccgcc gatttggggc tggcggagcg
     2101 gataaccttc cacggcaggc tgggacatgg cgaggtgatc agcctgttgc aacaggcaag
     2161 cctgttctgt tatccgacgg cggcttccga gggcttcccg aaatctgtgt tggaagcttt
     2221 ggcgtgcggg cttcccgcca tcaccactcg cgtctctgtt ttgccgcagc tcatcagtca
     2281 gggctgcggc atcctgctcg acgccccaac gcccgaagct gtcgccacag cgatccggcg
     2341 atgtgtagac caccaggatg agtaccggtt catgtcagcc cgggcgctgg agatcgcccg
     2401 cgaatactcc ctggaaaagt ggcgggagaa tatcgcctgt ttgttgcgag cagcatgggg
     2461 acctcttgcg tgtgacgcgt gatctcgcca ggctcaaaat ctgttttctc gccgggacac
     2521 tcggacaagg aggcgcagag cggcaactca ttcacattct gaaggcgctg cgctgccacg
     2581 gggcgggggt caggcttctc tgcctgacgc aaggcgaatt ttgggaagaa gccatccgca
     2641 agatggaaat tccggttatc tgggtcggcc ggcaaaagtc gcggctggca cgcttgggag
     2701 gcattttaag agtgctgaaa aatgaccctc ccgatatcat ccagagccac catttctata
     2761 ccaatctgta tgccacggcg gcagctcgaa taataggttc acgggatgtg ggagccctgc
     2821 gaaacgatcc cggcggtgaa atgaaatttc acgggtttgt gacggggcag ttaagtctcc
     2881 gggcgccgcg gctgctcgtt tccaattccc gcgctgcgat ggatagggcg agcgccctcg
     2941 ggatcccttc gtcgcgcctg cgatggctgc cgaacgttgt agatctcgat cgcttttgtc
     3001 ccggcggctg cgaacacaac gggcgtgttc gcctgctctc ggtgggaaga ctggaggaac
     3061 aaaagaggca tgaccggctt ctgagagctc ttaaaaaaca actcagcgga ccgtgcgggc
     3121 gcgattttcg tctcgttatc gccggagaag gctccgaacg gccgcgttta gaagcgctga
     3181 cccgaggcct tgacctgctc ccccgggtcc aatttcgcgg aggtgtcgtg gagaccgacg
     3241 ccttataccg ggaatccgac attttcgtcc tcgcttccga ctgggaggga acccccaatg
     3301 tcattcttga agcgatggcc tcggggctgc cggtggtagc cacccgcgtg ggaggggttc
     3361 cggaaatcgt ccagcatggg gaaacgggct acttagtgga tccggacgac gaaagctctc
     3421 tggcggaagc gctttcgatt ttgatcggcg attgccggct tcgtcgggaa atggggcgcc
     3481 gcgccaggaa gtatgtggaa gacaatcatt ctccgcagcg gcttggccgt cttttgcaag
     3541 agctgtacga ggcggcgctc tcgtgagaca gggcgttcat aagatgaccg cgaacaccgt
     3601 gcaaacctgg cgggtcccct tgctgcaaaa aacatccctg ccgatccgca agctgggctt
     3661 atttgccttg gcccacgtgc ctctgggatt gctgatgcat cagttcaacg cgatcgccac
     3721 ggtgcacgcg atcgtgacgt tcctgatcgg ttgcggatgg gctatctatg ggcggagacc
     3781 ggagcgggtg gcctatgtcg cggcttatat cacgggagcc gaagtgctgt ggcgcatggc
     3841 cagcgccgat gtattttggg aattcggcaa gtatgccgtg gccggcattt tcctcctcgc
     3901 actgctgcga acaggcaaat tacagcattt cgcgctgccg ctgctgtacc tcggtctgct
     3961 gaccccttcc acggtccaaa caatccagga tttgcggtgg gcggaagcgc gcagtcaaat
     4021 cagttttaat ctttcaggac cgttcgcact ggccatgagc tgctggtttt tcgccaacgt
     4081 tcgactttcg ctaaagcagc ttcagcgcct gtttctcgtg ctcattgcgc cgatcgccgg
     4141 aatcgcgacg atcgccctat acgggactgt agccgcttcc gaaatccatt tcaccaccca
     4201 gtcgaatttc gaaaccagcg gaggttttgg gcccaatcag gtttcggccg tgctgggctt
     4261 gggcgcgctc ctgacctttc tgatcctgct cacctaccag ggcaagaaag ccggcgtgaa
     4321 gctatgtctg ttctgcggtc tggcgttgtt cgccatccag agcgcgctga ccttctcccg
     4381 cggcggctta tacaatgcgg cgggggctgg cgccgtcgct tcgatgcttt tcctttcgaa
     4441 gaccaagttc aagctcaggg tgttagcggt cgccgtatta gtgctgatca tcgcgcacta
     4501 cgtggttctt ccctcgctgg atgactacac cggcggcgcg ctgtccgagc gcttcagcag
     4561 cttgaatttg accggcagag accgcctgat tcggggcgac ctgcaagcct gggaggagaa
     4621 tcctgttttc ggcgtcgggc ccggaagggc tcggttttcc cgtggcgagt tctaccggga
     4681 aggtgtggcg gcccacacag aattctcgcg cctcctcgcc gagcatggaa tgtgggggct
     4741 ggcagcactg ttcacgctgc tgttcatggc gatcaaaaac ttccggcagg ctcgcagcgg
     4801 tgtccataaa gcgctgctga ccgccgcgac cgcctggagt ctcttgttca tgaccaacgc
     4861 tgccatgcgg ctggtggctc cctcttttgt atttgggctg gcgtcggtga agttgctgcg
     4921 gcagcgaaag atccttcagt ggacgaccgg gccgtatccg agccatcaag gtcaggtcgc
     4981 ggtgaaacag attggcgctg tgaatccggc atttacccaa cgggcgccgg ggcgcctttc
     5041 gagcgcctat gaatgacagc cccgcggtgg ccgcacagcc taaagttgat tttggaccgc
     5101 cggcgcttct ctgcgcatct tcattttggg agtgtgctcc ggccggtttg cctgctgttt
     5161 gcttttggcg tagaaacccc gtgggaagac cggtgcgcat ttttgccgtc ggcgacacgg
     5221 ccttttcggg acgggtggcg gcaagcctcg aagggcggtc gtcgccaatc gccggcggcg
     5281 tggccgccct gctccagtcg ggcgacatcg tctttggcaa cctggaagga tcgctcagcg
     5341 atagcattcc gcgcggatcc ctcttcgccg cgcccgcatc cgcagcgtcg ttgctgtcgg
     5401 actccggatt caccctgctg catctcgcca acaatcacgc gtatgactat ggtcctgccg
     5461 gactgggatc cacgcttgcc gctttggcaa gcgccggcgt gacgccgctc ggcgcaggaa
     5521 ccgattcctt ctccgcccgg aggccggtcc ggacggatcg ccgagggctt cggatcggtt
     5581 ggctcggatg cggccgctcg gaatgcgcgc cagcgtctga cggcccccag ttttgggaat
     5641 ttgacgaagc agagctgttg caggcggtga ccagcgcccg cagagaagtt gattttctcg
     5701 tcgcttcgct gcacatgggc tttatgtatg ttgactaccc gcacccggac catcggactc
     5761 ttgccaggcg gctggcccaa gccggggctg acctcgttct tatgcatcac ccgcatgtgc
     5821 tccagggaat cgaaatcacg ccggaaggac ggggcatttg ctacagcctc ggcaattttc
     5881 tcctcgattg gcgggaaggc aacgtggcgc cggaggtgat gatcgccgag caaaaccagg
     5941 gcgccatctt attgttcgac gtggatcgtg aaggcgtttc actgatggcc gccctaccca
     6001 cacgcatcga ggccagaagc acggttcagt gggctacggg cgaggctggc cggaagattc
     6061 tccggcgcct ggcgcgcctc agtgacgatt tgaagggcga ttgcactgct tccttctgga
     6121 agcagcgttc cgagcggaac acggtcctgg ccgtgcgagt catgtggttc catttgtcgc
     6181 gcggccattg ggctgtggtg tggaacggcc tcaaaagagt tcgccgggag catctcacca
     6241 tgctcgcccg atggatccgg caatttttcc ggcatccggc tgaaactgcc gcgccgtgaa
     6301 tggcagctca atgcggagtt cccggacaag acttttgatg attggaaact tcctgtcagg
     6361 ctccgcaggc acgcgcgggg tttgcgagga cctggcggac cgcctgatct cggccggctg
     6421 gccggtgctg tttgcgtcgt cgaagcctgg aaagctgcgg cggctttggg acatgctgag
     6481 tacggcgcgg gcgcagcggg accggtatga cgtcgcgcac gtggacgtgt tcagcgggcc
     6541 ggcgttcttc tgggcggagc tggtctgctg gatattgcag cgcagccgaa agccttatgt
     6601 gctcacattg cacggcggca atctgccgga attcgcccgc cgctggcccc gccgcgtacg
     6661 gcgtctgctg ggatcggcca ccgccgccac ggcgccctcg ggatatctgc gcgaacaact
     6721 gcgggcctac cgcaaggatt tacaattgct gcccaatccg gtggatgtgg cgtcgtaccc
     6781 tttccggctc cgggagcggc ccacgccttc cctgatatgg ctgcgcgctt tccacgaaat
     6841 atacaacccc agcctggcag tgcaaacgtt agccttgctg acaacggatt tcccggacgc
     6901 ccaattgacg atgattgggc cggataaagg ggaccgcagc ttacagagtg cgcaacggct
     6961 ggccggcaag ctgggcctcg accgccaaat aacgttcgtc ccgggagtgc ccaagaccga
     7021 ggtgccgcgc cggctgctcg aagccgatat ttttcttaat acggcgaata tagacaacac
     7081 acccattagc ctcatcgagg ctatggcgag cggcatgtgt attgttacta caagcgtggg
     7141 cggtattcca tatctcctcg atcatgagaa agacgccctg ctggcgcagc caggcgaggc
     7201 ggaaagcatg gcgcatgggg tgcggcgcat cctgaccgaa gccgggctgg ccggaaagct
     7261 ttcccgcaac gcgcggcgca aggcccagca attcgattgg agactgattt ttccgcgatg
     7321 ggaagccctg ctgcgttccg ttgcggacaa gccggcggca tgagcaacgc ctggatccgg
     7381 atctatcatc gccttcctca accctgccgg tcggcggcgg ccgctgtgtg gggttttcgc
     7441 ctgcgcttct ggcggtacgg accggaatcg gaacagctcg tgactgaggc tctccaacgg
     7501 gattactggc agccgcaaca gtgggagaaa tggcgcgcag agcgcctggg ctacgtattg
     7561 catcgggcgg ctacccgcgt tccttactac cgcgagcaat gggaaatgcg gcggcggcgc
     7621 ggggaccggg cttcctggga ccggctggag aactggcccg tactgaaaaa agagatggtt
     7681 cgcgccaacc ctcgagcgtt cgtagccgat gattgcgacc cccggcggat gtttcacgat
     7741 cataccagcg gcacaaccgg cagcccgctc agcgtttggc tgaaccggaa cacagtacgg
     7801 gcctggtatg ccctcttcga ggcgcgctgc cggcggtggt acggcgtttc gcggttcgat
     7861 cgctggggca ttctcggcgg ccgactggtg acgcccgccg accagcgaaa accgccgttc
     7921 tgggtatgga acccggcgct caaccagcta tacttgtcgt cataccatct cgcccccgat
     7981 ctcacgccgt actatctgga agcgctggcg cggtatcgca tccagtatct tctcggctac
     8041 acatcctctc tttgcgccct ggcgcaggca gcgctgcgaa cagggcggcg aagcctgcct
     8101 ttgcgggtga tcgtcacaaa cgccgagccg ctgctgccgc accagcggca aatgatctcc
     8161 gaggcctttc aatgccccgt gcgggaaacc tacggcatga ctgagatcgt agccgccgcc
     8221 accgagtgcg agtgggggcg actccacctg tggccggagg ccggccgggt cgagattctc
     8281 gagcacgaca atccggtaca gcccggcgct tgcggcgata tagtcgcgac aggtttgctg
     8341 aacgccgata tgccgttgat ccgatatacg accggcgacc ggggcgcgct cgccgcgaac
     8401 aacattccct gctcctgcgg ccgcttgctg ccggtattgg cgtcggttga cggccgggcc
     8461 gacgacctct tgtacgctgc cgacgggcgc caaatcgggc ggatggaccc cgtcttcaaa
     8521 tccgatctgg ccgtgcggga agcccagatc attcaggaga ccctgggaac agtgcgagtc
     8581 cgttatgttc ctgcggcggg cttcaccgat cggtcggcga acacgatcat tgaagccata
     8641 cgctcccggc tgggtccggt tgaggttctt ttggaatccg tgccccaact tccccgcgaa
     8701 cctaacggca aattccgcgc ggtaatttgc cgcattcctc ctgaacaacg gcctgatggg
     8761 cagcggtgcg gcagtgagtt gacttcgacg ctggtatgac acgaaacgca gatctttccg
     8821 ctcagacaag ggtctccggg agcattcgga acccacagcc gatcgtttcc atcatcgtgc
     8881 cctgccgcaa cgaggtaaaa cacatccggg gctttctgga ctcggtcttt gagcaagatt
     8941 tggccggctt cgactgggag gtcatcgtag cggacggcat gagcgacgac ggcacatggc
     9001 cgttactcga agaatacgct cggaagcaac cgcggcttcg cctgttgaga aatccagccg
     9061 gaattgtctc gaccgggctg aacgcctgca tccgcgcggc gcggggcgat atcatcgtcc
     9121 gaatggacgc ccacacggaa tacgccccga actatgtgag ggagtgcctc cgggcgctcg
     9181 aggaaacagg ggcgggcaac gtcggcggac cggcccggac acgagcggat ggattgtggc
     9241 agcgagccat cgcggctgcc taccattctc ctttcgcctg cggaggcgtc agattccaca
     9301 atgactatta cgagggcttt gccgatacgg tgccttacgg ctgctggaaa aaattcaccc
     9361 tcgtggaact cggccttttc gacgagacgt tagtccggaa tcaggacgat gagctcaatc
     9421 tccggctgac gcgctccggc ggcaaagtgt ggcagacgcc gagaatcgtg tcttactatc
     9481 accctcggac tgaattgagc aagctattcc tccaatactt tcaatacggc ttttggaagg
     9541 tggccgtcat tcgcaagcat cgtttacccg cttcctggcg tcatttggtc cccggggcgt
     9601 ttgtggcggc gcttgggatc agctttttat cgtggctgat ctccgaaggc ggcgggcgct
     9661 tgggtttggc ggccgtttcc gaatcgctgc tgctaactct gctcctttcc tacgccgggt
     9721 gctgcgttat cgcggctttt acgtcagccc ggtcgcacgg ctggtcgatt tttccgatct
     9781 tgccaccgat tttcgccgtt tatcactttt cgtatggcct cggatttctt tgtggcctgg
     9841 cctgcgcgct gtttcctggc agccggctgt cgcggccggc cagagccttc gttgaaatca
     9901 gcagatgaga tgggcgagtc ggaaggctgc tgccaaagag aaaacaattc cgcttgcccc
     9961 gatcgcagca gctcaatgcc atcggcatac cctaccggca gcgagatcga gcgaattcgc
    10021 gcggaatatg cgcgccgcgg cagagaaatt ccacaggatt tttatagctg gagccgcccg
    10081 gtaaatcagt ttttccactg ccagaccctc cgcgcgtgcg ttgcggcctt ggttcgggag
    10141 gctttgtttc cacttactgg gaaaacaatt gtggacattg gttgtggctc gggagaatgg
    10201 cttaccgagt ttattcagtg gggcgctcga tgtgcggacg tcggtgggat cgatttgcgg
    10261 cccgataaga tcgaggaggc gcgcaagagg ctcccggagg ccaacctgca caccggcgat
    10321 gctcgccggt tgccatttcc gtctaactcg gctgacctgg tcagccagtt tacggtgttt
    10381 acctccatcc tggagccgca ttttaaaaag gcggtcgccg cagagatgct gcgggtgctc
    10441 aaaccgggcg gcctcatact gtggtacgat tttcgcttca acaatccaag caactccagc
    10501 gtccggggca tcaaatccga agagatccga acgctctttc ctgggtgccg gatccacttg
    10561 cgccgcgtca ccctggcgcc gcccgtcgcc cggttcgttg tcccgattac gtggattggc
    10621 gccttgctgc ttgagaaaat tccgatcttg agaacccatt atcttggcgc catccgcaaa
    10681 accgctattt agtcgtgagg cgccttcgaa acccgtacat tgcaagggcg cgaagaaatg
    10741 accaaagtcc tgctcgatcg aatgtgcgcg gcggctgctc tggtgctcct cttaccgctc
    10801 tggtttttta tcgccgtggc aattgcgtgg gacgacggcc ttccgattct gttccggcaa
    10861 acgcggatcg gccggggcaa tgttccgttc cagctcttga agttccgctc gatgcggccc
    10921 cgcgtgcccg ggccgcgcat cacggccggg aacgacccgc ggttgaccag ggtcggcgct
    10981 tttttgaggc gctttaaact cgacgaattg ccacagctct ggaacgtctt gaaaggcgac
    11041 atgagtctgg tgggcccgcg gccggaagtt ccggcgtttg tcgattttca ggatccaacc
    11101 tggcaaaccg ttcttcaggt ccggcccggg ataaccgatc ttgccagcct cgtttatcga
    11161 aatgaagaag agattctgtg cggccaaaat gacccagaaa gttactatcg caacgagatt
    11221 ctgccggcca agctggcgtt gaatatgcac tacctccggt cgcggtccct gtggtctgat
    11281 ttgaagatca tcctgatcac tatgcgatat tcatttttcc ctgccggttt tcattccagt
    11341 tctataagcc ggatgtttct caaacaggaa aattcgtgag cgagcaatct tttattccat
    11401 ttcatatccc cagcatcggc gaggaagaga tctccgaggc agcggcggcg ttgcgttcgg
    11461 gatggctgac cacaggtccg cggacggcgc agttcgagcg cgaattcaaa gagtacgttc
    11521 acgtttcgca tgctctggcc gtaaattccg gcactgcggc cctgcacctg gccctggctg
    11581 gcttggggat cggcccgggc gacgaagtca ttaccacccc gctgactttc tgcgccacag
    11641 tcaatacgat tctccaagtc ggcgcggtac ccgttttggc cgatatcggg gcggacggca
    11701 acatcgatcc ggcgtcggtg gcgcagcgca tcaccggccg cacgcgggct atcctccctg
    11761 tgcatctcgg agggctgccc tgcgaaatgg atcagctttg gtctttggcg agacaacatg
    11821 gcctgttcgt catcgaggat gccgcccatg ctgttggctc actttaccgg gggcagccaa
    11881 tcggcggcgg cagcgacgcc gtggcattca gcttttacgc cacgaagaac ttgaccaccg
    11941 gcgaaggagg gatggtcacc accaacaatt ccgcgcttgc ggagtcgatg cgaatcctgt
    12001 gccttcacgg gatcactaaa gacgcctgga accgctacgc gcagcacggt aattggtatt
    12061 acgaggttca ggcctgcggc ttcaaataca atctgagcga catacaatca gccatcggca
    12121 ttcatcaact tcgaaagcag gagcatttta ttgaagtccg tacacgatac gcccgcctct
    12181 acagtgaagc tcttgccggc gtgcccgaag tagacattcc ccccgacccg gaatggcgcc
    12241 gccattcgtg gcatttatac atactgcgcc tgaatctcga actgctggac atcaaccgcg
    12301 atgaattcat tcgcgaactg caccggaaag gcatcggagc cagcgtccat tttattcccg
    12361 tgccgctgca tccgtttttc aaggaatatg cgtcgcgtcc cgagaacttc tgtccccgag
    12421 ccttggcctt atatccgcgg ctggtgtcat taccgttgta tccggcgatg agcgaaaacc
    12481 aagtcagcta cgtctctcga acagtgaagg aaatcgtgac tgcggcgaaa cggcggaagc
    12541 cggtttttct ggccgctacg accccatgaa caccaagatt atcaagaagc tcttctaccg
    12601 ttttcggcga ttgtttatcg ccctatggca tcttgcgatc attaccgcag cactgctttc
    12661 cgcttttttg ctccgtttcg atttttcgat cccggcctcg gaagttccct tgctctttcg
    12721 tgtgttttgc attgccgtcg tgtcgaaaat gctggcattt tacctggcgg gaatcgaccg
    12781 cgggtggtgg cgattcgtgg gaatggccga tctgattcgc gtgctggttg ccaacgctat
    12841 gggatcctgg gctcttgctc tgatggcctc gtttctggtc gggtcccgct ttccggcttc
    12901 catctacttc atcgatttcc tgatctgctt tctggcgacg gccggcggac gcttctgcgt
    12961 ccgtctttac tatgaaacga tcgccggcga gttatccagg cccggccgcc ggcggctgct
    13021 gatttacggc gccggcatgg cgggggcgac agtcttgaag gaaatccggg cgaaccagag
    13081 cttgggttac gacgttgtcg gctttctgga cgatgacccc aagaaacgga acggcaggct
    13141 tcttggcgtc cccgtacttg gcgcggggag ggatgcttcc cgcattattg accattacaa
    13201 gcgaaacaac cggaacatag aagaaattct catcgccatg cccaccgcca ccggccgcca
    13261 gatgcaagaa
//
LOCUS       DASREK010000095         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1869743, whole genome shotgun sequence.
ACCESSION   DASREK010000095 DASREK010000000
VERSION     DASREK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869743"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFA54_04965"
     CDS             complement(<1..201)
                     /locus_tag="VFA54_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZS50189.1"
                     /translation="MISRKVEALSRTSLLASLTPGEIEALALRAIEKRYAPGEMLFRE
                     GEECTGFYVLAQGSVKIVKTSPG"
     gene            303..2111
                     /locus_tag="VFA54_04970"
     CDS             303..2111
                     /locus_tag="VFA54_04970"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HZS50190.1"
                     /translation="MSEKLIHRAMPLPEILVKYPGCRQILDRYGLLGCGGPYGPQEPL
                     WFFARAHRIPEAQLLCELEQAAQRGADWCAGPANDADSAAVIYRRFFKAAIVVMFTFG
                     CVFGGINLAILAAKHQLASLDMRALIWSHAHAQVAGWVTFFVMGFAYQAFPRFKLTAL
                     RRPRLAAAIPYVMAPALAVRCLADLWQKAPFWKVAGITAGAVELAAVLAFVWIITQTL
                     RSSQQAREPYEKFIYAALGWMVVAFAFDLWVFAASSAISGYSEWVRFIGAYDGPWRDL
                     QLLGFAGGMILGVSQRFLPFLYGFGEVSPRASNRAFYLWNLSVAGNIAFYSLLIHTRR
                     PAWGAMLELSLLGLLAAVLILIRSFHLYSENREQDRSLPFIRAAYGWAAFALALLMLM
                     PLYHFAVGRVFSHAYFGAYRHAFTVGFISMMIVGVSSKVVPILAGINARERRSLAAPF
                     WLLNTGNGMRVLFQILTDTQPWAYPWMALSAWIEVAGLIWWAIDLWRTMDHKPSQARG
                     LAGAVTVTPQTLVSDIISRYPAAAAVFEAFGFSLINNPVARLAVRSVTLQQACRLKHI
                     QYEVLAPALLQCIEDSEKEPRATHRTSQLQGLAALL"
     gene            2191..>2675
                     /locus_tag="VFA54_04975"
     CDS             2191..>2675
                     /locus_tag="VFA54_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS50191.1"
                     /translation="MPGQTQSSSSGLQLLLATISFTICFAAWGLISAFAPYFREMFRL
                     SATDTALLVSVPVLLGSLGRIPMGMLADRFGGRVVFSLLMLFAAGPAFLAPKAESHRE
                     LLALGFLLGMAGSSFAVGVQFVSGWFPADRQGAALGVYGLGNIGQSGAVFLGPVLASV
                     IG"
BASE COUNT          418 a          839 c          800 g          618 t
ORIGIN      
        1 gccgggcgaa gttttgacga tcttgacgct tccctgcgcc agcacgtaga acccggtgca
       61 ctcttctccc tcccggaaca gcatttcgcc gggcgcgtag cgtttttcaa tcgcccgcag
      121 cgccagcgcc tcgatctcgc cgggagtgag gctggcgagc aaagaagtcc tggagagggc
      181 ttccactttt cgcgagatca caggcgcacc cattcggcta ccgacgaatg taccgcatgc
      241 ggcgtgtatc ctcaaggctg tccgaacggc gccagcgcca cgccgcggca ggagagcatt
      301 gtatgagcga aaaactcatt caccgcgcga tgcctcttcc ggaaattctc gtcaagtacc
      361 ccggttgccg ccaaatttta gaccggtatg gcctgcttgg ttgcggcggg ccttatggac
      421 cgcaggagcc gctgtggttc tttgcccggg ctcaccgcat tccggaagct caactgctct
      481 gcgaactcga gcaggcggcg cagcgcggtg cagactggtg cgccgggcct gccaacgacg
      541 cagattctgc ggcagtgatc taccgccgct tcttcaaggc cgccattgtg gtcatgttta
      601 cgttcggctg tgtcttcggc ggcatcaatc tggccattct ggccgccaaa catcagctcg
      661 cctcgcttga catgcgcgcc ctcatctggt cgcacgcaca cgcccaagtg gccggatggg
      721 tgactttctt cgtcatgggc ttcgcctatc aggccttccc gcgattcaag ctgacagcgc
      781 tccggcggcc tcgcctggcg gctgccattc cttatgtgat ggcgcccgcg ctggccgtgc
      841 gttgcctggc tgacctgtgg caaaaggccc cgttttggaa ggttgccggc attaccgccg
      901 gcgcggtgga gttggcggca gtcctcgcct ttgtctggat catcacccaa actctccggt
      961 cctcccaaca ggccagagaa ccgtacgaaa agtttattta cgcggcgctg ggctggatgg
     1021 tcgtggcatt tgcatttgat ctttgggttt tcgcggcatc ctctgccatc agcggctaca
     1081 gcgaatgggt gcgattcatt ggcgcttacg acgggccgtg gcgcgacctg caactgctgg
     1141 gtttcgccgg cggcatgatt ctcggcgtga gccagcgttt cctgcccttc ctctacggct
     1201 tcggtgaagt ctccccaagg gcctcgaacc gggcttttta cttatggaac ctgtccgtgg
     1261 cgggaaacat cgcattttac tccctgctga ttcatacgcg ccgcccggca tggggcgcca
     1321 tgctcgagct gagcttgctc gggttgctgg ccgctgtcct gattctgatt cgcagcttcc
     1381 acctgtattc cgagaatagg gaacaagatc gctcactgcc tttcatccga gccgcctatg
     1441 gttgggccgc tttcgcgctt gctttgctga tgctgatgcc gctctaccat ttcgcggtgg
     1501 gcagggtttt ttcccatgct tatttcggcg cctaccggca tgccttcacc gtcggcttca
     1561 ttagcatgat gattgtgggc gtctcgagta aagtcgttcc catcttggcc ggaatcaacg
     1621 cgcgagagag gagatcgctc gccgcccctt tctggctgct gaataccggc aacggcatgc
     1681 gcgtgctctt tcaaatcctg accgatacgc agccctgggc ctatccctgg atggcgctga
     1741 gcgcctggat agaggttgcc gggctgatct ggtgggccat cgatctgtgg cgcaccatgg
     1801 atcacaagcc ctcccaggcg cgagggctgg ctggcgcagt cacggtgact ccgcaaaccc
     1861 tggtatcgga catcatcagc cgttatccgg cggcggccgc cgtctttgaa gcgttcggct
     1921 tttcactgat caacaacccg gtagcgcgcc tggccgtacg ctccgtgacg ctccagcagg
     1981 cgtgccgtct caaacatata cagtacgagg tgctggcgcc tgccctgctc cagtgcatag
     2041 aggactccga aaaggagccg cgcgcgacgc accgaaccag ccaactgcaa gggctggccg
     2101 ccctcttgta atcctgtctt cggtatcatc gaaagtcgaa agggttgcaa agatgcttcg
     2161 aagcggaaca aggagcgctg tctgactctg atgccaggac aaacacagtc ttcgagctcc
     2221 ggcctccagc ttctgctggc caccatctcc ttcacgatct gcttcgctgc ctgggggctg
     2281 atcagcgcct tcgctccgta tttccgcgaa atgttccggc tgtccgcgac cgatacggcg
     2341 cttctggtat ccgttccggt gctgctcggc tcgttgggcc ggatccccat gggcatgctg
     2401 gcggaccgct tcggcggtcg tgtcgtgttc tctctgctga tgctctttgc cgccgggccg
     2461 gcattcctgg cgcccaaagc agagagccac cgcgaacttc tggcgcttgg ctttttgctc
     2521 gggatggcgg gttcctcgtt tgcagtcggc gtccagttcg tttccgggtg gttccccgcg
     2581 gaccggcaag gtgctgcgct gggcgtttat ggtctcggca atatcggcca atcgggggcg
     2641 gtttttctgg gtccggtgct ggcgtctgtc atcgg
//
LOCUS       DASREK010000096         9244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2362975, whole genome shotgun sequence.
ACCESSION   DASREK010000096 DASREK010000000
VERSION     DASREK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2362975"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VFA54_04980"
     CDS             <1..142
                     /locus_tag="VFA54_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZS50192.1"
                     /translation="RPEKGMSEKLANELNDAANLRGGAIKKRDDVHRMAEANRAFAHY
                     RW"
     gene            214..2307
                     /gene="fusA"
                     /locus_tag="VFA54_04985"
     CDS             214..2307
                     /gene="fusA"
                     /locus_tag="VFA54_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZS50193.1"
                     /translation="MARTIPLERMRNIGIAAHIDAGKTTTTERILYYTGRTHKLGEVH
                     EGTAIMDWMEQEQERGITITSAATTCQWRDIQINIIDTPGHVDFTAEVERSLRVLDGA
                     VAVFDAVAGVQPQTETVWRQADKYSVPRICFINKMDRVGADFEHSIETIRQRLKANPV
                     AIQYPIGAEDRYKGLVDLIAMKAIFWRDETLGAEFDVEEIPEELRQVCREYRERMIEA
                     VSEQDEKLFEKFVHGEPVSNEELKAGLRRATIAMKLFPVVCGAAFKNKGVQPLLDAVV
                     DYLPSPLDIPPIRGTSLDGKEVIERRADDKEPFAALVFKIMTDPFVGQLAFLRVYSGV
                     LKSGDSVYNVAKQRKERIGRLLRMHANKREEIDEALAGDIAAVVGLKTVQTGDTVCDE
                     NHPVVLESIEFPTPVVSVAVEPKTRADQQKLGMALAKLAQEDPTFKVTTDPETNQTII
                     SGMGELHLEIIIDRMMREHSVSANVGKPQVAYRETIRKAAEGEGRFVRQTGGRGQYGH
                     VKITVEPLPQGSGFQFENAIVGGAIPKEFIKPVEAGIQEALESGILAGYEMVDVKATV
                     YDGSYHEVDSSEMAFKIAGSMALKDAVRKANPVLLEPIMSVEIVVPEEYMGDVIGDIN
                     SRRGRIEGMELRGGSQIIRAVVPLAEMFNYTTDLRSRTQGRGSHTMHFARYEEVPAAV
                     AEEIISRVQGKVVRS"
     gene            2426..3613
                     /gene="tuf"
                     /locus_tag="VFA54_04990"
     CDS             2426..3613
                     /gene="tuf"
                     /locus_tag="VFA54_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZS50194.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHIDHGKTTLTAAITKVLAKKDPKVQ
                     FRSFDSIDNAPEEKARGITIAVAHVEYSTEKRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPYIVVFLNKVDAVDDPELLELVELEVRELLT
                     KYGFPGDKLPVVRGSALGALNGDPKWEATVEELMKAVDEYIPLPQRDIDKPFLMPIED
                     IFSISGRGTVVTGRIERGKIKVGEEVEIVGFRDTMKRVVTGVEMFKKLLDEGQAGDNV
                     GLLLRGTEKDEVERGQVVAKPGSITPHKKFRGEVYVLSKEEGGRHTPFFNGYRPQFYF
                     RTTDVTGVAQLPEGTEMVMPGDNVSLTVELITPVAMEKGLRFAIREGGRTVGAGTVSE
                     ILE"
     gene            3680..3832
                     /gene="rpmG"
                     /locus_tag="VFA54_04995"
     CDS             3680..3832
                     /gene="rpmG"
                     /locus_tag="VFA54_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZS50195.1"
                     /translation="MPREIITLQCSECKNRNYTGTKNKKTKTERLELKKFCRHCRKHT
                     AHREIK"
     gene            3902..3976
                     /locus_tag="VFA54_05000"
     tRNA            3902..3976
                     /locus_tag="VFA54_05000"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3935..3937,aa:Trp,seq:cca)
     gene            3999..4250
                     /gene="secE"
                     /locus_tag="VFA54_05005"
     CDS             3999..4250
                     /gene="secE"
                     /locus_tag="VFA54_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZS50196.1"
                     /translation="MAKEATEGVTGAVETVKGWPEKVKNYIEGLRMEMRRVTWPNKKQ
                     VQATTIVVIITVFLFGAFFAVVDSILQFLVTRLFNYFTR"
     gene            4271..4990
                     /gene="nusG"
                     /locus_tag="VFA54_05010"
     CDS             4271..4990
                     /gene="nusG"
                     /locus_tag="VFA54_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZS50197.1"
                     /translation="MNETEKTAGEPAKEDREKAVNEETADAEVSRNPSPQEKASAGEK
                     SPAETADGGAPEGSKAAAHPGMGWYIIHTYSGFENKVKESLRSRAEAFGYGDKIGEIL
                     IPTEDVVELRAGKKVTSKRLIYPGYVLVQMEMNDELWHVVKNTPRVTGFVGGGNVPTP
                     LSADEVNQILYRQATAADKPKPKMTFTKNETVKITDGPFTNFTGKVDEINADRNTLRV
                     LVTIFGRATPVELDFFQVEKM"
     gene            5046..5474
                     /gene="rplK"
                     /locus_tag="VFA54_05015"
     CDS             5046..5474
                     /gene="rplK"
                     /locus_tag="VFA54_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZS50198.1"
                     /translation="MAKKVKSEIKLQIMAGKATPAPPVGPALGQAGVNIVDFCKAFNA
                     KTASKDLEGLIIPVVITVYTDRSYSFITKTPPVAVLVKKAVNLAKGSGEPNKNKVGKI
                     TRKQIEEIARQKMPDLNCHTLEAAMRCVEGTCRSMGVDVA"
     gene            5573..6271
                     /gene="rplA"
                     /locus_tag="VFA54_05020"
     CDS             5573..6271
                     /gene="rplA"
                     /locus_tag="VFA54_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZS50199.1"
                     /translation="MKPGKKYQAAAKQVEQRKYKLEEAVPLLQKIKYAKFDETVELHM
                     RLGVDPKHADQMVRGTVVLPNGLGTSKRVLVIASGEKIQEAERAGADFAGGEEMVNKI
                     QQENWLDFDAVVATPDMMRHVGRLGKVLGPRGLMPNPKTGTVTMDVAQAVKEIKAGKV
                     EFRVDKTGIIHAPVGKISFQADKLVENAASLIQSIIKAKPAAAKGRYVHSVTLCSTMS
                     PGVSIDVSAMETRA"
     gene            6350..6874
                     /gene="rplJ"
                     /locus_tag="VFA54_05025"
     CDS             6350..6874
                     /gene="rplJ"
                     /locus_tag="VFA54_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZS50200.1"
                     /translation="MKSKAKKKEAVEQLHKELAKTQNAFTASFQGIKVHEDFELRKQV
                     RAAGGRYRVVANRLARVAAQGTPAEPLLKDLKGMTSLVLADQDPVALAKALTAYAKNN
                     PVFSFRAGMVAGKVVDVNQIAALANLPGKEAIYAKLLFLINAGAQRLVTAVNAVGRNL
                     AVVIDQGVKEKKFS"
     gene            6977..7363
                     /gene="rplL"
                     /locus_tag="VFA54_05030"
     CDS             6977..7363
                     /gene="rplL"
                     /locus_tag="VFA54_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZS50201.1"
                     /translation="MADIHAIAEQIQGLTLLEAAELVKVLETKLGVSAAAAAPVMMAG
                     GGSAAAPAAESAEEKTEFTVVLTSAGASKINVIKAVREVTNLGLKEAKDLVDGAPKVI
                     KENVSKQEAEELKKKFTEAGATVEIR"
     gene            7421..8464
                     /locus_tag="VFA54_05035"
     CDS             7421..8464
                     /locus_tag="VFA54_05035"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZS50202.1"
                     /translation="MRSLTAKERKKTGRPARRSFFQTLLGSAVAASLAKKILAQTSSN
                     GGGIPTRPLGRTGERVSILALGGWHIATAKDKGEAIRIMHAAIDEGITFFDNAWDYHD
                     GRAEEWMGEALASGGRRQQVFLMTKNCNRDYAGSLKCLEDSLRRLRTDHLDLWQFHEL
                     VYDNDPDWIFEKGGIKAAFEARKAGKVRFVGFTGHKDPRIHLKMLSQDYPWNASQMPV
                     NVMDAHYRSFQREVVPVCLRNNVGVIGMKGLAGGSPRGRIPTATAITAEECLRYALSL
                     PVASLVVGIRSMEDLRQDVAVARNFRPMADLEKTRLLSRVREEAGDGRHELFKSTQQF
                     DSPHHRRQHDFEV"
     gene            8893..>9244
                     /locus_tag="VFA54_05040"
     CDS             8893..>9244
                     /locus_tag="VFA54_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50203.1"
                     /translation="MDARLDGAPRERLDFSKIKTTIPIPNLIEVQKKSYERFLQMDLL
                     PSEREDTGLQAVFNSVFPITDFRGVSQLEFVDYSIGNWECKCGNLRGLHHLRSTCKNC
                     GATIRTDPYYAGEIP"
BASE COUNT         2073 a         2380 c         3148 g         1643 t
ORIGIN      
        1 ccggccggaa aaagggatgt cggagaagtt ggcgaatgag ttgaacgacg cggcgaatct
       61 gcgcggagga gcgatcaaga agagagacga cgtgcaccgg atggcggagg ccaaccgggc
      121 attcgcgcat taccgatggt aggcaaacga cgtggaccgg gaggtccgcg ccaccgtgtt
      181 gtggcaggaa gggtcaggtt ttagaagttt tttatggcaa gaacaatccc gctcgaaagg
      241 atgagaaaca tcggcatcgc ggctcatatc gatgctggta agaccacaac tacggagcgg
      301 attttgtatt acacggggcg gacgcacaag ctcggcgaag tccatgaagg cacagccatt
      361 atggactgga tggagcagga gcaggagcgc ggcatcacga tcacttcggc cgcgaccacc
      421 tgccagtggc gcgacatcca gatcaacatt atcgatacgc cgggccacgt ggatttcacc
      481 gccgaagtcg agcgcagcct gcgcgtgctc gatggcgcgg tggccgtctt cgacgcggtg
      541 gccggtgttc agccgcagac cgagactgta tggcggcagg ccgacaagta ttccgtcccc
      601 cgcatttgct tcatcaacaa gatggaccgc gtgggcgccg atttcgagca ttcgatcgag
      661 acgatccgcc agcgcctgaa ggccaatccc gtggccattc aatatcctat tggcgcggag
      721 gaccgctaca aaggcctggt cgatctgatc gcgatgaagg cgattttctg gcgggatgag
      781 acgctgggcg ccgagtttga tgtggaggag attccggaag agctccggca agtctgccgg
      841 gagtaccggg agcggatgat tgaggccgtc tccgagcagg atgagaagct gttcgagaaa
      901 ttcgtccacg gagaaccggt cagcaacgaa gagcttaaag ccggcctgcg gcgcgcgacc
      961 atcgccatga agctgtttcc ggtcgtttgc ggagcggcgt tcaagaacaa aggcgttcag
     1021 ccgctgctcg atgccgtggt ggattatctg ccttcgcccc tggatattcc cccgatccgg
     1081 ggaacgagtc tggacggcaa agaggtgatc gagcggcgcg ccgacgacaa agagccgttc
     1141 gccgcgctgg tgttcaagat catgacggac ccgtttgtcg gccagttggc cttcttgcgg
     1201 gtgtattcgg gcgttttgaa aagcggtgac agcgtatata acgtcgccaa acagcgcaaa
     1261 gagcggattg ggcgcctgct gcgcatgcac gccaataagc gtgaagagat cgacgaagcg
     1321 ctcgccggcg acattgcggc ggtggtcggg ctgaagacgg ttcagaccgg cgacacggtt
     1381 tgtgacgaga atcatccggt ggtgcttgaa tcgatcgagt ttccgactcc ggtcgtatcg
     1441 gtggccgtcg agcccaaaac gcgggccgac cagcagaaac tgggtatggc gctggcgaaa
     1501 ctggcacagg aggatccaac ttttaaggta acgaccgatc cggagacgaa ccagacgatc
     1561 atctccggca tgggcgaact gcatctggag atcatcatcg accggatgat gcgggaacac
     1621 tcggtgtcgg cgaacgtcgg taagccgcaa gtggcctacc gtgaaaccat tcgcaaggcg
     1681 gccgaaggcg aggggcgctt cgtccgacag accggcgggc gagggcagta cgggcatgtg
     1741 aagatcacgg ttgagcctct gccgcaggga agcgggtttc aatttgaaaa tgcgattgtg
     1801 ggcggcgcga tcccgaagga gtttatcaag cccgtcgagg ccggcattca ggaggctctg
     1861 gagagcggga ttctggccgg ctacgagatg gtcgacgtaa aggccaccgt atacgacgga
     1921 agctaccacg aggtggacag ttccgaaatg gctttcaaaa tcgccggctc catggcattg
     1981 aaggacgcgg tccggaaagc caacccggtg ctgttagaac ccattatgag cgtcgagatc
     2041 gtcgttcccg aggaatacat gggcgatgtg attggagaca tcaattcccg gcgggggcgg
     2101 atcgaaggaa tggagttgcg cggcggatcg cagattatcc gcgcggtcgt gccgctggcc
     2161 gagatgttta actacaccac cgaccttcgt tcccgcacgc aaggccgcgg cagccacacc
     2221 atgcactttg cgcggtatga agaagtgccg gccgctgtgg ccgaggagat catcagccgg
     2281 gtgcagggaa aggtggtccg gagctgaagg gagcgcggac cggaacctct gggaaaggcg
     2341 ggtgtaggga tcgggcagtg gagtttgctg gcagggagac gcgatcgtcg gcggatgagc
     2401 gctgcgagcg ggagcgaggg acataatggc gaaggaaaaa tttgatcgga gcaagccgca
     2461 cgtaaacgta gggacgatcg ggcacatcga tcatggcaag acgacgttga cggcggcgat
     2521 cacgaaggta ctggcgaaga aggatccgaa ggttcagttc cggtcgtttg attcgatcga
     2581 caatgcgccg gaggagaagg cgcgggggat cacgatcgcg gtggcgcacg tggagtattc
     2641 gaccgagaag cggcactacg cgcacgtaga ttgtccgggg cacgccgact acatcaagaa
     2701 catgatcacg ggtgcggcgc agatggacgg ggcgattctg gtggtggcgg cgaccgacgg
     2761 gccgatgccg cagacgcggg agcacatatt gctggcgcgg caggtagggg tgccgtacat
     2821 cgtggtgttt ttgaacaaag tggatgcggt ggacgatccg gagcttttgg aactggtgga
     2881 gttggaagtg cgggagctgc tgacgaagta cgggtttccg ggggacaagc tgccggtggt
     2941 gcgggggtcg gcgctgggag cgttgaacgg ggacccgaag tgggaagcga cggtggaaga
     3001 gctgatgaag gcggtggacg agtacatacc gctgccgcag cgggacatcg acaagccgtt
     3061 tttgatgccg atcgaagaca tattctcgat ctcggggcgg gggacggtgg tgacggggag
     3121 gatcgagcgg ggcaagatca aggtagggga agaagtggag atcgtgggat tccgggacac
     3181 gatgaagcgg gtggtgacgg gcgtggagat gttcaagaag ctgctggacg aggggcaggc
     3241 gggggacaac gtaggtctgc tgctgcgagg gaccgagaag gacgaagtgg agcgggggca
     3301 ggtggtggcc aagccgggtt cgatcacgcc gcacaagaag tttcgcgggg aagtatacgt
     3361 attgagcaag gaagaagggg ggcgtcacac gccgtttttc aacggatacc ggccgcagtt
     3421 ttattttcgg accacggacg tgaccggggt ggcgcagttg ccggaaggga ccgagatggt
     3481 gatgccggga gacaacgtga gcctgacggt ggagttgatc acgccggtgg cgatggaaaa
     3541 gggactgcgg ttcgccatcc gtgaaggcgg ccggacggtc ggcgccggca ccgtgtcgga
     3601 gatattggag tagccgggaa aggatggggc ccggagactt gtggtctggt gcgatgggcc
     3661 gccgcaacag agagtcggta tgccgagaga gattatcacg ttgcagtgca gtgagtgcaa
     3721 gaaccgcaac tatacgggca cgaagaacaa gaagacgaag acggagcggc tcgagctgaa
     3781 aaaattttgc cgccattgcc gcaagcatac ggcgcatcgg gagattaaat agagcagggt
     3841 tgtgcaggtt ggccggaagg ggttgtcgcc ccaatcggca gcggacctca acccggcgtg
     3901 caggggcgta ggttgaacgg tagaccagcg gtctccaaaa ccgcgagtgg gggttcgaat
     3961 ccctccgccc ctgccactga aaagaagcga tggctaccat ggcgaaagaa gcgaccgaag
     4021 gcgtaacggg agcggtggaa accgttaagg gctggcccga gaaagtcaag aactatatcg
     4081 agggcctgcg gatggaaatg cggcgcgtca cctggccgaa caagaaacag gtacaggcga
     4141 ctaccatagt ggtcatcatt acggtttttt tgttcggcgc cttttttgcc gtggtcgatt
     4201 ccattttgca gtttttggtg acccgcctgt tcaattactt cacgcgttag tttggcaggg
     4261 aagcattaac gtgaacgaga ctgaaaaaac cgctggcgag ccagcgaaag aagaccggga
     4321 aaaggccgtc aacgaagaaa cggcggacgc cgaagtgagc cggaatcctt ctccgcagga
     4381 aaaggcctct gccggggaga agagtccggc cgagacggct gacggcggcg cgccggaagg
     4441 gtccaaggcc gcagcccacc cgggcatggg ctggtacatc attcatacgt attccggttt
     4501 tgagaacaag gtgaaggaga gcctccgcag ccgggccgag gcgtttggct acggggataa
     4561 gatcggcgag attctgatac caaccgaaga cgtcgtggag ttgcgcgccg gaaagaaggt
     4621 gacgagcaag cggctgattt atccgggcta cgtcctagtg cagatggaaa tgaacgacga
     4681 gctctggcac gtggtgaaaa acacgccgag agtcacggga tttgtcggcg gcggcaatgt
     4741 gcctacgccg ctcagcgccg atgaagtcaa ccagattctc taccgccagg cgacggcggc
     4801 cgacaagccg aagcccaaaa tgacttttac caagaacgaa acggtgaaga tcaccgacgg
     4861 gccgtttacc aacttcaccg gcaaggtgga tgaaatcaac gccgaccgga acacgctgcg
     4921 ggtgctggtg actatcttcg gccgcgcgac gcccgtagaa ctggatttct tccaggtgga
     4981 aaagatgtag ccggctgggc tctctgcgga gacccgggcc tcgggcgcaa taattgcttt
     5041 gacatatggc aaaaaaagta aaaagcgaga tcaagctgca aatcatggcg gggaaggcca
     5101 cacccgcgcc gccggtaggg ccggcattgg gtcaggccgg agtcaatatc gttgacttct
     5161 gcaaggcctt caacgccaag acggcgtcga aggatctgga gggcctgatt attccggtcg
     5221 taatcactgt ctatacggat cgttcgtaca gcttcattac gaagacgcct ccggtggccg
     5281 tgctggtaaa gaaggccgtc aatctggcca agggctccgg cgagccgaat aagaacaagg
     5341 ttggcaagat cacgcgcaag cagatcgagg agatcgcccg gcagaagatg ccggacctca
     5401 actgccacac gctcgaggcg gccatgcggt gcgtcgaggg aacttgccgc agcatggggg
     5461 tggacgtagc ctgacgccgc gaggcgggcg cgcagggaat tccaaaggca ggcacaaacc
     5521 gggatgaacg aagtgggagg ccctaaacgg ccgcaactac cacgaggtac acatgaaacc
     5581 aggtaagaag tatcaagcgg cagccaagca ggtcgagcag cgcaagtaca agctggaaga
     5641 ggctgttccg cttcttcaga agatcaagta cgccaaattt gacgaaaccg tcgagctgca
     5701 catgcgcctg ggcgtggacc ccaagcatgc ggaccaaatg gtgcgcggga ccgtcgtgct
     5761 gcccaacggt ttgggcacgt caaaacgagt cctcgtgatt gcttccggtg agaagattca
     5821 ggaggcggag cgggccggcg ctgatttcgc aggcggggaa gaaatggtca acaagatcca
     5881 gcaggaaaac tggctggatt tcgatgccgt cgtcgctacg cccgacatga tgcgccacgt
     5941 cggcaggcta ggcaaggtgc tcggaccgcg gggcctgatg cccaacccga agaccggcac
     6001 ggtcactatg gatgtggcgc aagccgtcaa ggaaatcaag gccggcaagg tggaattccg
     6061 tgtggataag acggggatca ttcacgcgcc ggtcggcaag atcagctttc aggctgacaa
     6121 gctggtcgaa aacgccgctt cgctgattca atctatcatc aaggccaagc cggcggccgc
     6181 gaagggcagg tacgtgcaca gcgtcactct gtgctcgacc atgagcccgg gcgtaagcat
     6241 tgacgtttcg gccatggaaa ccagagccta gaggcatggt tctcacggtt gccggggacg
     6301 tctcccatca cgcggagcgg aagcgccctt taatggaaga gagacgaaca tgaaatcgaa
     6361 ggcgaagaag aaagaagcag tcgagcagct ccacaaagaa cttgccaaga cgcagaacgc
     6421 attcaccgct tcgttccagg gcatcaaagt gcacgaggat ttcgagctgc gcaagcaggt
     6481 gcgggctgcc ggcggaagat accgtgtcgt ggccaatcgc ctggcccggg tggcggcgca
     6541 aggaactccg gccgagcctt tgctgaagga cttgaaggga atgacatcgc ttgttctggc
     6601 cgaccaggat ccggtcgctt tggccaaagc cctcaccgcc tatgccaaga acaacccggt
     6661 gttttccttc cgcgccggaa tggtcgcggg caaggttgtt gacgttaacc agatcgcggc
     6721 cctggcgaac cttcccggca aggaagcgat ctacgccaag ctgttgttcc tgattaatgc
     6781 gggagcgcag cgcctggtca cagccgtgaa tgcggtcggc cgcaatctgg cagtggtcat
     6841 agatcaggga gtgaaggaaa agaaattttc atagctttca gttcccagcg accggttttt
     6901 caacgccagc gcgcgggcgt gcagcgctga ctgctggcag aactgacggc ttccaattta
     6961 aggagcattt ggcaaaatgg ctgatattca tgcaattgca gagcagatcc agggtctcac
     7021 tcttctggag gccgcggagc ttgtgaaggt tcttgagacc aaactgggcg tttcagcggc
     7081 ggccgctgcg ccggtcatga tggccggcgg cggcagcgcg gcggccccgg cggcggagtc
     7141 ggcggaggaa aagacggagt tcactgtcgt tcttaccagc gccggcgcca gcaagatcaa
     7201 tgtgattaag gccgtccggg aagtcacgaa cctgggactc aaagaggcga aagacctggt
     7261 ggatggggcg ccgaaggtaa ttaaagagaa cgtctccaag caggaagcag aggaactcaa
     7321 gaagaagttc accgaagccg gggcgacagt cgagatcaga tagcgttcca gggccggccg
     7381 gaaaggcggg agcctggccg gtttgtctgc gggtggacgg gtgcgctctt taactgcaaa
     7441 ggaacgtaag aagaccggca ggcccgcgcg gcggagcttc tttcaaaccc tcctggggag
     7501 cgccgttgcc gcgtcgctgg ccaagaagat actagcgcaa acatcctcga atggcggcgg
     7561 cattccgacg cgtcccttgg ggaggaccgg agaacgggta tccatcctgg cgctgggcgg
     7621 atggcacatc gcgacggcga aagacaaggg cgaagcgatc cggatcatgc atgccgcgat
     7681 cgacgaaggg atcaccttct tcgacaacgc ctgggattat catgacgggc gcgccgagga
     7741 gtggatgggt gaggcgctcg catcgggcgg ccggcggcag caggtgtttc tgatgaccaa
     7801 gaactgcaat cgggactacg cggggtcgct gaaatgcctg gaggacagcc tgcgccggct
     7861 gcgtaccgat catctggacc tttggcagtt ccacgagctg gtttacgaca acgatccgga
     7921 ttggatattt gaaaaaggcg gcatcaaagc ggcgttcgag gcccgcaaag ccgggaaggt
     7981 ccgtttcgtt ggcttcaccg ggcacaagga tccccggatc cacctgaaga tgctcagcca
     8041 ggattacccc tggaacgcgt cgcaaatgcc ggtaaatgtg atggacgcgc actatcgcag
     8101 ctttcagaga gaggtcgtgc cggtttgctt gcgaaacaac gtcggcgtga tcgggatgaa
     8161 gggtttggct ggcggctctc ctcgtggccg gatacccacg gcgacagcca tcacagccga
     8221 ggagtgcctg cggtatgcct tgagccttcc cgtggcgtcg ctcgtggtgg gaatccggtc
     8281 gatggaagat ctccggcagg atgttgccgt tgcgcggaac ttccggccca tggcggatct
     8341 cgaaaaaaca agattgctga gccgggtgcg ggaggaggct ggagacgggc ggcacgaatt
     8401 gttcaagtcc acacagcagt ttgacagccc gcatcaccgg cgacagcacg attttgaggt
     8461 ttaggggggt tccggacaca gacccggcgc ttggccgggg ccgggcaggc gcgcgcttgc
     8521 gccgggcgcg cctttggtgt tagactggtt tttcggtgct ggtgttggga ttccactcgc
     8581 tggattcgtg gtggccgaca acttttctga gggatggctt cgcacttcgc tagaagctgc
     8641 gcggaggcta cgcctttttg ccgccgggca cggggttggt gtgtctggcg gagcctgtcc
     8701 cgctctcgct cgggtcctaa ctgtcatggc tcttttgaat cgggagcagc ccctcccgtt
     8761 ttctcctgcc cgtgtactgc cgctgtgtgg cgaagcggcc ggccctgtct gggatggccg
     8821 ctgatcgagt gattgatctc gcatggtgag gccggcgctt cggcccatat ctgtgtttca
     8881 ggagcttttc taatggacgc aagactcgat ggcgctcccc gcgaacgcct ggatttttcg
     8941 aagatcaaaa cgacgatacc aattcccaat ctgatcgagg ttcagaaaaa atcatacgag
     9001 cggttcttgc aaatggacct gctgccgagc gagcgggaag acacaggtct gcaagcggta
     9061 ttcaactcgg tttttccgat cacggacttc cgcggcgtga gccagttgga gtttgtggat
     9121 tactccattg ggaactggga gtgcaaatgc ggcaacttgc gcggcctgca ccatctgcgg
     9181 tccacttgta agaactgcgg cgcgaccatt cgcaccgatc cctactatgc cggcgaaatt
     9241 ccct
//
LOCUS       DASREK010000097         3997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1042843, whole genome shotgun sequence.
ACCESSION   DASREK010000097 DASREK010000000
VERSION     DASREK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042843"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFA54_05045"
     CDS             <1..634
                     /locus_tag="VFA54_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS50204.1"
                     /translation="YLRSLAFDKAERAAAERVYARHLGKAPPPDAEYKALQDFLFRYI
                     ASECGRLGMAVHLHAAAGAGSYFDVAGANPLLLQSVLNDPALRKTNFVIVHGGWPFTR
                     ETTALLQKPNTYLDYSAQSLLLPPATLAPTIREWLEWAPEKVLFGTDAYPYSDELGWE
                     ESEWLAAKRGREALAIALTAMVRDGEISKDRAFELARMVLRENARKLYGL"
     gene            complement(692..1153)
                     /locus_tag="VFA54_05050"
     CDS             complement(692..1153)
                     /locus_tag="VFA54_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50205.1"
                     /translation="MALRHRASAGEKAPVRELARVLLLEDDVTVRLALQAILEASGYS
                     VDSAASAPEAMDKLEREQYALVLCGLHDGARDECRRVISHARAQDYKPATAYLTSSGG
                     QNKPPKSGRLLIEPQEVPALLTKIADLLADRAAGRERRNMRRRRVDLVGIN"
     gene            complement(1229..2545)
                     /gene="purB"
                     /locus_tag="VFA54_05055"
     CDS             complement(1229..2545)
                     /gene="purB"
                     /locus_tag="VFA54_05055"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZS50206.1"
                     /translation="MIPRYTRPEMGRIWTDQNKFDTWLKVELAAAEALAEMGEIPHEA
                     AAALARHAAFDVPRILEIERDVKHDVIAFTTAVSEKMAAAGQAEASRWLHYGLTSNDV
                     VDTAQALQISQASALIAVGLERLSEVLKARALEFRDTVCIGRTHGVHAEPTTFGLKLA
                     NWYAEVSRQIARFAEAREQMRVGKISGAVGTFAHIGPEAEERICAKLGLKPDPISTQV
                     IQRDRHAHYVSTLALIAATLEKIALEIRHLQRTEVREAEEFFSKDQKGSSAMPHKRNP
                     ITCEQICGLARVVRANAIAAGENIALWHERDISHSSVERVILPDSTILVDYLLARTTN
                     IISTLVVYPERMRQNLEMTQGLVFSGQLLLDLAARGALREEAYRWVQRNAMRAWESGE
                     SFRHLVEQDLDISKYLNIKDLDNTFSLDRQLRNVGRIFERVFGPGS"
     gene            complement(2736..3512)
                     /locus_tag="VFA54_05060"
     CDS             complement(2736..3512)
                     /locus_tag="VFA54_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS50207.1"
                     /translation="MSVRICVLGSGSGGNCTFVATERTRILVDAGFSYREIKTRLRKI
                     GEDPERLDAVLITHEHSDHVAGLPVLARKLSNPIFLTRLTESRLEWGEARPPVEIFQA
                     GQRISIGDFEIDSFTVPHDAIDPVAFCFRAEGLKLGVITDLGYMPESVKHQIRGCDFL
                     VLESNHDLDMLRVGPYPWFVKQRVMSRVGHLSNHAVSEFLTADYDRASQVLVLAHLSE
                     QNNHPEIARLTAIQALQAAGAEATRLVLAEQWRPTEMFQF"
     gene            3760..>3997
                     /locus_tag="VFA54_05065"
     CDS             3760..>3997
                     /locus_tag="VFA54_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50208.1"
                     /translation="MKKLVFFTVAVLLANAISPVLLGGIPSDKAVYRGGTIAALPENT
                     EGAVTTTNEQYFLFTYKKGRLLIPYERVNFLEYGQ"
BASE COUNT          829 a         1227 c         1165 g          776 t
ORIGIN      
        1 gtatctgcgt tccctggcat tcgacaaagc ggagagagcc gcggcagagc gcgtatacgc
       61 ccgccatttg ggaaaagccc cgccgcccga cgccgagtac aaggcgcttc aggattttct
      121 tttccgctac atcgcctcgg aatgcggccg tcttggcatg gcggttcacc tgcacgcggc
      181 ggccggagcg ggcagctatt tcgatgtggc cggagcgaat ccgctgctgc tgcaatccgt
      241 tctcaacgac cccgcattga ggaagacgaa tttcgtaata gtgcacggcg gctggccatt
      301 taccagggaa accacagcgc tgctccagaa acccaatacc tacctggact actccgccca
      361 atccctgctg ctgccaccgg cgacgctggc tcccaccatt cgggagtggc tggaatgggc
      421 gcccgaaaag gtcctcttcg gcacagacgc ttacccctat tcggacgaat tgggatggga
      481 ggaatccgag tggctggcgg caaaacgcgg gcgcgaggcc ctggcgatcg ccctcaccgc
      541 catggtgcgg gacggagaga tttcaaaaga ccgggctttc gagctggccc gcatggtgct
      601 gcgcgagaat gcccgcaaac tgtacggcct atagcagccg ggcggcgcga gcactgatgg
      661 gaaaaaagaa aatgactaag gaaaaaccga atcagttaat cccgaccaaa tcaacccgcc
      721 tccggcgcat attccggcgc tcgcggccgg ccgcacgatc tgcgagaagg tcggcgattt
      781 tggtcagtaa ggcggggact tcctggggtt cgatcaacag ccggccggat ttgggcggct
      841 tattttgccc tcccgaactg gtcaaatacg cggtggccgg cttgtaatcc tgagcgcggg
      901 cgtggctgat cacacggcgg cactcgtcgc gggccccatc gtgcaatccg cacagcacca
      961 gcgcgtactg ctcccgctcg agtttatcca tggcctccgg cgcggaggca gccgaatcga
     1021 ccgagtatcc gcttgcctcc agaatcgcct gcaaggccag gcgaactgtc acatcgtctt
     1081 ccagcaacaa tacacgcgct agttcgcgga ccggcgcctt ttctccggcg ctggcccgat
     1141 gcctcagggc catcccaaaa agctccttat ggcaacaatt ctactccact ttgttcggat
     1201 tttcgataga aaaacctgag ccggcaaact aagaccccgg gccgaagacg cgctcgaaaa
     1261 tccggcccac gttccggagc tggcgatcca gggaaaatgt gttatctaag tcttttatat
     1321 ttaaatactt agatatatcc aagtcctgtt ccaccagatg ccggaagctc tcgccgcttt
     1381 cccaggcccg catcgcattg cgctggaccc atcggtacgc ttcctcgcgc agcgcgccgc
     1441 gggcggccag atcgagcagc aattggcccg agaaaaccag tccctgggtc atctccaggt
     1501 tttggcgcat gcgctccgga tagacgacta aggtgctaat aatgttcgtg gtacgcgcca
     1561 acagataatc caccaaaatt gtcgaatcgg gaagaatgac gcgttcgacc gacgagtgcg
     1621 aaatatccct ttcgtgccaa agcgcgatat tttcgccggc ggcgatggca ttagcccgca
     1681 ccacgcgcgc cagcccgcag atctgttcgc acgtgattgg gtttcgttta tggggcatgg
     1741 ccgaactgcc tttctgatcc ttcgagaaaa actcttcggc ctcccgcact tccgttcgct
     1801 gtagatgccg gatttcgaga gcgattttct ccaaggtagc ggcaatcagc gccagggtcg
     1861 aaacgtaatg cgcatggcgg tcgcgctgga tcacctgagt agaaatcggg tcgggcttga
     1921 gccccagttt ggcgcagatc cgttcttcgg cttcgggccc gatgtgcgca aacgtgccca
     1981 cggctccgga aattttgccc acccgcatct gctcgcgggc ttccgcaaag cgcgcgatct
     2041 gacgcgagac ttcggcgtac cagttggcca gcttgagtcc aaaggtggta ggctccgcgt
     2101 gcactccatg ggtccggccg atacagaccg tatcgcggaa ttcaagagcc cgggccttca
     2161 ggacctcgct caggcgttcc agcccgacgg cgatcagggc cgaagcctgc gagatctgaa
     2221 gcgcttgcgc cgtatcgacg acatcgttgg aagtcagccc gtaatgcagc cagcgggatg
     2281 cctcggcctg gcctgcggcc gccatcttct cggagacggc ggtggtgaag gcgatgacat
     2341 catgtttcac gtcgcgctca atttcgagga tccgcgggac gtcgaaagcg gcgtgccggg
     2401 cgagcgccgc agccgcttcg tgcggaattt cacccatctc ggccaaagcc tccgcggccg
     2461 ccagctctac cttgagccaa gtgtcaaatt tgttctgatc ggtccagatg cgccccattt
     2521 ccgggcgcgt gtagcgggga atcatagcga aagttcaacc cttattggcg ccgccgagcg
     2581 cttcgttctt acttcgggcg acagcggatg tgcaaagcgg ccacggcaag ccggcatccg
     2641 ggcatgaatt caaccgctgc gccggcgcgc gagcaaacca tagtatcagc aaaccgcgcc
     2701 ggggcggttc cgcgaggcgg atgccagagc ttgcctcaaa actgaaacat ttccgtgggc
     2761 cgccactgct cggccaggac aagccgggtg gcttcggcgc cggcggcttg cagcgcctga
     2821 atcgcagtca ggcgtgcaat ttccgggtga ttgttttgtt cgctgaggtg agccagcacg
     2881 agcacctgag acgcgcggtc gtagtcggct gtcagaaact cggaaaccgc gtggttggaa
     2941 agatgcccca cacggctcat cacccgctgc ttcacaaacc acgggtatgg cccgactctc
     3001 agcatatcga gatcatgatt cgattcgagc accaaaaaat cgcagccgcg gatctggtgc
     3061 ttcaccgatt ccggcatata acctaagtcg gtgatcacgc ccagcttgag cccctcggcg
     3121 cggaaacaga aggccaccgg atcaatagca tcatggggaa cggtaaacga atcgatctca
     3181 aaatcgccga tggaaatcct ctggccggcc tggaaaattt cgaccggcgg ccgcgcctcg
     3241 ccccactcca gtcggctctc cgtgagccgt gtcaggaaaa tcggattgga aagcttccgc
     3301 gccaggaccg gcaggccggc gacatggtcg ctgtgctcat gggtgatcaa gacggcgtcc
     3361 agacgctccg gatcctctcc gatctttcgc agcctggttt tgatctcacg ataactgaag
     3421 ccggcgtcca ccaaaatccg cgtccgctcg gtggctacaa aagtgcagtt gccgccgctg
     3481 ccgctgccca ggacgcagat ccggacgctc atggttgcac gcttctcatt gtttgtatca
     3541 gcatttcgaa ccaatcacaa tagttttcga gcgccggccg ggggacgagc ggcagacatt
     3601 ggccgccggt gcgctctgcc ggcgatgaac cacagacagg gccctcgaat gcagaaattc
     3661 gaaaagctgg aaaatcccgc tggatccgct ggcccttgcg cttttcggtt gggaaaggca
     3721 aaataggcac cgacagattt gttcgcggag aggacgccga tgaaaaagct ggtctttttc
     3781 acggtggcgg ttctccttgc gaatgccatt tcgccggttc tgctgggtgg catccccagc
     3841 gacaaggctg tctatcgcgg cggcaccatc gccgcgttgc cggaaaacac cgaaggagcc
     3901 gtaaccacca ccaacgagca atactttctg tttacctaca agaagggacg cttgttgatc
     3961 ccttacgagc gggtaaactt cctcgagtac ggccaaa
//
LOCUS       DASREK010000098         4782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_386179, whole genome shotgun sequence.
ACCESSION   DASREK010000098 DASREK010000000
VERSION     DASREK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386179"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFA54_05070"
     CDS             complement(<1..317)
                     /locus_tag="VFA54_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50209.1"
                     /translation="MCKLERIGLCTVLALGPAVMALHAQIGNRTQPTGALALEDNAGI
                     LVCERVSNVWNPGMYSAGMLQQNAFQKTGPGVITPAGTITWNEAALAAEEGRGPNQYP
                     DQGP"
     gene            complement(426..2255)
                     /locus_tag="VFA54_05075"
     CDS             complement(426..2255)
                     /locus_tag="VFA54_05075"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZS50210.1"
                     /translation="MVRRWGVWLAWTAFLLPAFSATVSFDLRDAEGRRHTIAEAHESK
                     AIVLLFLAPDCPISNSYAPELNRLAAQYSRRGVLFYGVFSDPAVSGTEMEQYGREYGY
                     SFPLLLDRSQSLARQAGAAATPEAVILSARGEVLYRGRIDNRFIDFGKTRPAPARRDL
                     KLSLDQILAGKPVSHPATKALGCGIPFGREIHGEAVTFARDIAPIVYQHCAPCHHPGG
                     AAPFPLLRYEDVTKRASQIAAVTSSGYMPPWKPQPGYGKFRGERGLTEAEVTAIRDWV
                     NEGAPLGKTATPPAPKFASGWQLGTPDLIAKMPEPFTVPAEGPDIYQCFVIPITLPAD
                     RYVRAVEFQPENRSAVHHALFFADLSGGARRRDAEDPAAGYRCFGVPGFLPSAALGGW
                     SPGVAPLETPAQTAVPLKKGSDLVLQIHFHPTGKQEREQASLGLYFTSVPPQRRVADV
                     ALGSRNIDIPPGDRSYKVRDGFTLPIDVEAVGIIPHAHYICKDMKGWALLPDGTKKWL
                     IWIKDWDFNWQEQYQYGPSLHLPAGTRLEMEFTYDNSNQNPRNPNHPPKRVLWGPEST
                     DEMAGLHLQVIPERMSEMPILGQALWGKIMRSMGGKFFTLPSQ"
     gene            complement(2301..2777)
                     /locus_tag="VFA54_05080"
     CDS             complement(2301..2777)
                     /locus_tag="VFA54_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50211.1"
                     /translation="MTRRYCLFVCATAAGILLAAPLSPQMRRGSSARRGRQQQAPGAP
                     ESVPPADFSGTLRKLDKKEILLEVEPDRVLNLRRTGKTKFLKGSAAVQPSAFKNGDHI
                     SVEATREVDGSLTAVAVFLSPPPAEPGKQSKPEPNPGGSETSGDGASASHGPAPAR"
     gene            2847..>4782
                     /locus_tag="VFA54_05085"
     CDS             2847..>4782
                     /locus_tag="VFA54_05085"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50212.1"
                     /translation="MRLSRGLLAGLLCVLMPALASEIAVTGRIVDENGAAVGGARITF
                     KKAGDSTSQDVLADPTGTFNVRLPAPGAYSVAAAQTGFFPLKEYSIQVTEGPQEVHLV
                     LNHIREVFQSVNVETSVSPIDFDRTTSERTLDKLQIMNVPYPSTHDLRNAIKLIPGVV
                     QDASGGLHFDGSAENQVLYTLDGFNISDPLTGRFSARLSVEAVRSLEYSSGRFSPEFG
                     KGSAGALAIQTETGDDQWRYSATNFVPGIDTLKGIHIGAWTPRFNVSGPLKKGRAWIS
                     DNMDGEFSELLIPDAPPGQDRSSTWRGDNLLHTQFNATPSNLLYADFLINGASSSNLG
                     LSALDPISTTTDQRLRTWFYSLKDQIYLGRGAMLELGFGENRTFLRQIPQGHDFYVMT
                     PAGRRGNYFIDSVQKSRRDQFLANLIPPAFHLAGSHQFKSGIDWDRLNYWQDIRRTGF
                     EQLDLSGRLLRLTTFVGNGRFSRPGLEASSYLLDDWRPKPNLLLELGVRQDWDELVRR
                     TVLSPRLSFSYAPFGSKNTKIAGGYAVVHDATSLQLFTRPMDQYSVSTTYNPDGTVAS
                     GPSLTIFTIQNPHLTVPRYQNWTLGFERQLPRKFDLRLNWLRKRSHDGFTYINSLGSS
                     SLVLPQPVAGYDASSLAGVFN"
BASE COUNT          912 a         1472 c         1409 g          989 t
ORIGIN      
        1 ggcccctgat cgggatactg gttcggcccg cgtccttctt cggccgcaag cgccgcttcg
       61 ttccaggtaa tcgtccccgc gggtgtgatc acgccgggac cggttttttg gaatgcgttc
      121 tgttgcagca ttccggcaga gtacatgccg gggttccaaa cattcgagac gcgctcgcaa
      181 accagaattc cggcattatc ctccagcgca agcgcccccg ttggctgggt ccggttcccg
      241 atttgggcat gcagagccat gacggcggga ccgagcgcca ggacagtaca caatccgatc
      301 cgctccagct tgcacataac agccacctcg cagtgagaga tgtgcagcgc tggtttgcgt
      361 ttccaacaga tttgttatgg aaggccctga atgtcaggtt aagcccgatc tggcgaagca
      421 tcttttcact ggcttggtaa tgtaaagaac tttccgccca tgctgcgcat gatctttccc
      481 caaagggctt gccccaaaat cggcatctct gacatgcgct cgggaattac ctgcaaatgc
      541 aggccggcca tctcgtcggt gctttccggg ccccagagca cgcgcttggg cgggtggttc
      601 ggattgcgcg ggttttgatt cgagttatcg taggtaaatt ccatttcgag gcgcgtgccg
      661 gccggcaaat gcagagatgg gccgtattga tactgctctt gccagttgaa gtcccaatct
      721 ttgatccaga tcagccactt tttcgtgcca tcgggcagca gagcccagcc cttcatgtcc
      781 ttgcaaatgt agtgggcatg aggaatgatt cctaccgcct ccacgtcgat gggcaaggta
      841 aagccgtccc ggactttgta agagcgatca ccgggcggga tgtcaatgtt gcgcgatccc
      901 agcgcgacgt cagcgaccct cctttggggt ggaacgctag tgaagtacag gcccaggctg
      961 gcctgctccc tttcctgctt gcccgtcgga tgaaaatgaa tttgcaggac cagatcggag
     1021 cctttcttta gaggcaccgc ggtttgcgcg ggcgtctcga gaggcgccac gcccggcgac
     1081 cagccgccga gagccgccga aggcaggaac ccaggcaccc cgaagcagcg atacccggcg
     1141 gcagggtcct ccgcgtctct cctccgcgct ccgccggaaa gatcagcgaa aaacagagcg
     1201 tggtgtacgg cgctgcggtt ctcgggttgg aactcgacag ctctgacgta tcgatcagca
     1261 ggaagtgtta tgggaatcac gaagcactgg tatatatccg gcccttcggc cggaacggta
     1321 aaaggctcag gcatcttcgc gatcaagtca ggcgttccaa gttgccagcc ggaagcgaac
     1381 ttcggcgcag gcggagtcgc cgtctttccc aggggcgcgc cttcgttgac ccagtcccga
     1441 atggccgtca cctccgcttc ggtcagcccg cgctctcccc gaaacttgcc atagcccggc
     1501 tgcggcttcc agggcggcat ataaccgctg gatgtgaccg cggcgatctg ggaggcgcgt
     1561 ttcgtaacgt cttcatagcg cagcaaggga aagggcgcgg cgccgccggg atggtggcaa
     1621 ggggcgcagt gctgatagac aatcggagcg atgtcacgcg cgaaagttac tgcttccccg
     1681 tgaatctccc gcccgaacgg aatgccgcat ccaagcgcct tcgtcgcggg atgggagacc
     1741 ggcttgccgg ccaaaatctg atcgaggctg agcttcagat cgcggcgcgc tggcgccggg
     1801 cgcgttttgc cgaaatcgat aaaccggttg tcaatccgtc cgcggtaaag cacttcaccg
     1861 cgggctgaga ggatcacggc ttcgggcgtg gctgccgcgc cggcctggcg cgccagagat
     1921 tgcgaccggt cgagcagaag cgggaaggag tagccgtatt cccggccata ctgttccatt
     1981 tccgtcccgc tgacagccgg atcggaaaac acgccataaa acagcacgcc gcgccgcgag
     2041 tattgcgctg cgagccggtt taattccggc gcatagctgt tggaaatcgg acagtcgggc
     2101 gccaaaaaca ggagcacaat agctttgctt tcgtgcgcct cggcgatggt gtggcggcgg
     2161 ccctcagcat cccgcagatc gaaggagacg gttgcggaaa acgcaggaag caagaaggcc
     2221 gtccacgcca gccatactcc ccaacgcctt accatgtcga tctccatcgt tccacgaaac
     2281 ggcgcctggc ccgcacaaat ttaccgcgcc ggcgcggggc cgtgggaggc ggaagccccg
     2341 tcgccgcttg tttcgctccc gcctggattt ggttccggtt tggattgttt ccccggctcg
     2401 gccggcggcg ggctcagaaa cacagcaaca gcggtgagcg atccgtcaac ctcgcgcgtt
     2461 gcttccacgg aaatgtggtc gccgttcttg aacgccgaag gctggacggc ggcagaacct
     2521 ttcagaaact tcgtctttcc cgtgcggcgg agattcagca cgcggtccgg ctccacttcg
     2581 agcagaatct cttttttgtc cagtttgcgg agagttccgc tgaaatccgc cggcgggacg
     2641 ctctccgggg cgcccggcgc ctgctgctgc cgcccccggc gtgcgctcga accgcgccgc
     2701 atctgcgggc tgaggggcgc agcgagcagt atgcctgcgg ccgtcgcaca tacgaaaagg
     2761 cagtatctgc gagtcatcgc tttcagttta aacccgaaat ttacctgctt ctctcgggga
     2821 tgtatactga gctttcaggt gcggctgtga gattatcccg gggcttgttg gcgggactgt
     2881 tgtgcgtcct tatgccggcc ctggcgtccg agatcgctgt gaccggccgc attgtggacg
     2941 agaacggcgc agccgtgggc ggcgcgcgca taacgttcaa aaaagccggc gactccactt
     3001 cgcaagacgt tctggccgac cccacaggaa ctttcaatgt ccggcttccc gcgccgggcg
     3061 cttattctgt cgccgctgca caaaccggat ttttcccttt gaaggaatat tcgattcaag
     3121 tgacagaggg gcctcaggag gtgcacctgg tgctaaatca tatccgggaa gtttttcagt
     3181 cagtgaacgt cgaaacctcg gtgtcgccga ttgacttcga ccggaccaca tcggaacgca
     3241 cgctcgacaa actccaaatc atgaacgttc cgtacccttc cacgcatgac ctgcgcaacg
     3301 cgatcaagct gatccccggt gtggtgcagg acgcgagcgg cggcctgcac ttcgacggca
     3361 gcgccgaaaa tcaggtgctg tatactctgg atggcttcaa catcagcgat ccgctgaccg
     3421 gccggttttc cgcgcgcctc agcgtggagg cggtccggtc gctcgagtat tcttcgggcc
     3481 gcttctcgcc ggaatttggc aagggctcag ccggggccct cgccatccaa accgaaaccg
     3541 gagacgatca gtggcgctac tcggcgacga acttcgtgcc cggcatcgat acactgaagg
     3601 gcatccacat cggcgcctgg actccgcgat tcaatgtctc cggccctttg aaaaaaggcc
     3661 gcgcctggat ttccgataac atggacggcg agttttccga gctgttgatt cccgatgcgc
     3721 cgccgggaca ggaccgttcc tctacctggc gcggagacaa tctgctgcac acgcagttca
     3781 acgccacgcc atctaatctc ctttatgccg attttttgat caacggcgcc agttcctcca
     3841 atctcggctt gagcgcgctc gatccgattt ctaccaccac cgatcagcgc ctgcgcacct
     3901 ggttctacag cctgaaagac cagatctatt tggggcgcgg cgctatgctg gagctgggct
     3961 tcggcgaaaa ccggacgttt cttcgccaga taccccaagg gcatgatttc tatgtaatga
     4021 cgcccgcggg gcggcgcggc aattacttca tcgactccgt ccaaaaatcg cggcgcgatc
     4081 agttccttgc caacttgatt cccccggcct ttcatttggc gggatcgcac cagttcaagt
     4141 ccggcatcga ttgggaccgc ctcaattact ggcaagacat ccggcgcacc ggtttcgagc
     4201 agttggatct cagcggcagg ctgctccgcc tgacaacctt tgtcggcaat ggacggttca
     4261 gccgcccggg cctcgaggca tcctcttatc tgctggatga ttggaggccc aaaccgaatt
     4321 tgctgttgga acttggcgtt cgccaggact gggacgaact ggtgcgcagg acggtcctct
     4381 cgccccggct ctccttctcg tacgccccct tcggctcgaa aaatacgaag atcgccggcg
     4441 gttacgccgt cgtgcacgat gcaaccagct tgcagctatt tacccgccca atggatcagt
     4501 actcagtcag cactacttac aatccggacg ggacggtcgc aagcggccct tctctgacga
     4561 tcttcaccat tcagaatccg catctgaccg tgcctcgata ccagaactgg actctcgggt
     4621 tcgagcgcca gttgccgagg aagttcgatc tgcggttgaa ttggttgcgc aaacgcagcc
     4681 acgacggctt tacctatatc aacagcctcg gctcgagcag tctggtattg cctcagcctg
     4741 ttgccggcta cgatgcgtcg tcgttggccg gagttttcaa tc
//
LOCUS       DASREK010000099         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1377162, whole genome shotgun sequence.
ACCESSION   DASREK010000099 DASREK010000000
VERSION     DASREK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377162"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFA54_05090"
     CDS             complement(<1..1135)
                     /locus_tag="VFA54_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS50213.1"
                     /translation="MDRRQFLKDTAKAAALATSAVSMSARAYSRVLGANDRIRLGQLG
                     CGDRSRGHVHMAQLAAKHTPVEHVAVCDLWSKARERRAAQVKQAFGVEPKVFKYYEDM
                     LAQNDIDGVMIATGDFQHAKLCAEVVRAGKDCYVEKPFANVLAEAKECRAVVKASKQV
                     VQMGSQHRTQPYQIAVRDIVRSNRIGPIVKIEQEWNVNMPRWHQRQAAIRELREEDTD
                     WKRWLLGKPYRPFDPRVYLEFRLFKDFSGGIFDQWMSHGADLVHLWTDSTHPVSCVAT
                     GGIFVWKDGRENPDTATAVFTYPKGFLYSYSTTFGNSYRSFSRIFGREGTIENYGGEG
                     ASLFMVTREGGPHEPDPEGSPHKPEYFAPPGMGPSDEKPEIVRV"
     gene            complement(1158..>2572)
                     /locus_tag="VFA54_05095"
     CDS             complement(1158..>2572)
                     /locus_tag="VFA54_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZS50214.1"
                     /translation="QISLVTAARENWAVSLQGQAPRTLAGGQAANIRFRVTAPENAEY
                     TRPYFQRHNVEQPYYEILKPEYANLPLPPYPLSAQLDLLYEGAPVRLAQVVQSVKRVT
                     GRGVVMNPLVVGPAISVSAAPRTGILPLDAKSFLLTANIHSNVKGPAKGTVKLELPQG
                     WQSLPATAEFSTSKDGEEQMLSFRVMPSRLQERPYTVTVVAEYNGRQYKEGYRAVGYE
                     GLRPYHLYRPASYTATGVDVKAAPGLNVGYVMGSGDEIPECLENLGIKVHMLVSADLA
                     TGDLGKYDVILLGVRTYAVREDLKTNNSRLLDYVKNGGVVIVQYNTPEFDHNFGPYPY
                     TMTGDPEEVTDERSKVEILEPGNPVFNWPNKITAKDFEGWVAERGSKFLSQWDPRYQP
                     LLETHDPGQDPQKGGMLYARYGKGVYIYNAYAFYRQLPEGVPGAYRLFANLVSLPRNP
                     QFRSAASGRPGAKRPAHSGV"
BASE COUNT          479 a          805 c          766 g          522 t
ORIGIN      
        1 caacgcgcac aatctcgggc ttctcgtcgc tgggccccat acccggagga gcaaagtatt
       61 ccggcttgtg cggcgagcct tccggatccg gctcgtgggg gccgccctcg cgagtgacca
      121 tgaacaacga cgcgccttcg ccgccatagt tctcaatcgt gccttcgcgg ccgaaaatgc
      181 ggctgaagct gcgatagctg ttgccgaacg tcgtggaata gctgtatagg aagcccttcg
      241 ggtaagtaaa cactgcggta gccgtgtccg ggttttcgcg gccgtccttc cacacgaaaa
      301 tcccgccggt cgcgacgcag cttaccggat gcgtggaatc ggtccacagg tgaaccaggt
      361 cggcgccgtg gctcatccac tggtcgaaaa tgccgcccga aaaatccttg aacaggcgga
      421 actcgaggta gacgcgcggg tcgaacgggc gatacggctt gcccagcagc cagcgcttcc
      481 agtcggtgtc ttcttcacgg agctcccgga ttgccgcctg ccgctggtgc cagcgcggca
      541 tattgacgtt ccactcctgc tcgattttca cgatggggcc gatccggttg gagcgcacga
      601 tgtcgcgcac ggcgatttga tagggctggg tgcggtgctg gctccccatt tggaccactt
      661 gctttgaagc cttcaccacc gcgcggcatt ctttcgcttc ggcaagcacg ttggcgaacg
      721 gcttctcgac ataacaatcc ttgcctgccc ggaccacctc agcgcacagc ttggcgtgct
      781 ggaagtcgcc cgtcgcgatc attacgccgt cgatgtcgtt ctgggccagc atgtcttcat
      841 aatatttaaa caccttgggc tccaccccga acgcctgctt cacctgggcg gcgcgccgct
      901 cgcgggcttt gctccacaag tcgcacaccg cgacatgctc gaccggcgtg tgcttggcag
      961 cgagttgagc catgtgcaca tggccgcggc tgcggtctcc gcagcccagt tgtccgaggc
     1021 gaatacggtc gttggcgccc agcacgcggc tatatgcgcg cgcgctcata gagacagccg
     1081 aagtagccag cgccgccgct ttcgctgtgt cctttaaaaa ttgccggcga tccataaatt
     1141 cgtcctcctc cttacgatca tactcctgaa tgcgcgggcc gcttcgcccc gggccggccg
     1201 gaagccgcac tgcggaactg cgggttcctc ggcaacgaga ccaggttggc gaacagccgg
     1261 taggcgccgg gaacgccttc cggcaactgc cggtaaaatg cgtaagcgtt ataaatatac
     1321 acgcccttgc cgtagcgggc gtaaagcatg ccgccctttt ggggatcctg ccccggatcg
     1381 tgcgtctcga gcagcggctg ataccgcgga tcccactggc tcaggaactt tgagccgcgc
     1441 tcggccaccc agccttcaaa atctttggcg gtgatcttgt tgggccagtt gaaaacagga
     1501 ttcccgggct cgagaatttc aacctttgac cgctcgtcgg tcacttcttc cggatcgccg
     1561 gtcatcgtat aaggatacgg gccgaagttg tggtcgaact cgggcgtgtt gtactgcaca
     1621 atgacgactc cgccattctt cacgtaatcg agcagccggg aattgttggt cttgagatcc
     1681 tcccgcacgg cgtaagtccg gacgcccaga agaatcacat cgtatttgcc caaatcgccc
     1741 gtcgccagat cggcggaaac cagcatgtgg actttgattc ccaggttttc gaggcactcg
     1801 ggaatctcgt cgccgctgcc catgacgtag ccgacattga gaccgggcgc cgctttgaca
     1861 tccacgccgg tggccgtgta gctggccggg cggtagagat gataggggcg caagccctcg
     1921 tagcccacgg ctcgatagcc ttccttatat tgccggccgt tatactcggc gaccacagtc
     1981 acggtatagg gcctctcctg caatcgggag ggcatcacac gaaagctcag catctgctct
     2041 tcgccgtcct tcgaagtgga aaattccgct gtcgccggca aggactgcca cccctgcggg
     2101 agctcgagct tcaccgtccc tttggcgggc cctttcacgt tgctgtgaat gttcgcagtc
     2161 agaaggaagg atttcgcatc cagcggaagg atccctgttc ggggagcggc ggacacggag
     2221 atcgccgggc cgacaaccag cggattcatc acgacccccc ggccggtgac ccgtttaacc
     2281 gactgaacca cttgcgccag gcgcaccgga gcaccctcat agagcagatc tagctgcgcc
     2341 gaaagcggat agggcggcag cggcaaattc gcatattccg gtttcagaat ctcgtaatag
     2401 ggctgctcga cattgtggcg ctgaaaataa ggacgcgtgt attcggcgtt ctcgggcgcc
     2461 gttacccgga agcggatatt agcggcctgc ccgccggcaa gagttcgcgg cgcctggcct
     2521 tgcaggctga ccgcccagtt ttcgcgggcc gcggtgacaa gagagatctg at
//
LOCUS       DASREK010000100         3883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_57157, whole genome shotgun sequence.
ACCESSION   DASREK010000100 DASREK010000000
VERSION     DASREK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57157"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFA54_05100"
     CDS             <1..517
                     /locus_tag="VFA54_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50215.1"
                     /translation="DSAQVILHQSLREQYEWMASYTRSRAYSNAVLDITIDQPMLISS
                     NVGPMPWDSPNRFLSWGYLPTPHKNWALAYLLEVRDGFPFSLVNQQGQVIGGVNASRL
                     PMYFNLNLHLERRFRLGSYHFALRGGFNNATNHRNPTVVNNTLGSPQFLTYYGSEGRH
                     FVFRLRWLGKD"
     gene            701..1027
                     /gene="groES"
                     /locus_tag="VFA54_05105"
     CDS             701..1027
                     /gene="groES"
                     /locus_tag="VFA54_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZS50216.1"
                     /translation="MKFRPLHDRVLVRRIEEAETVRGGIIIPDTAKEKPQEGEAIACG
                     NGKLLDTGQRVALEVKPGDRVLFGKYSGNEIKLDGEDFLILREDEILAILDKNSSGSD
                     NKKGKK"
     gene            1043..2665
                     /gene="groL"
                     /locus_tag="VFA54_05110"
     CDS             1043..2665
                     /gene="groL"
                     /locus_tag="VFA54_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688391.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZS50217.1"
                     /translation="MPAKKIILGEDSRQSILRGVNALADTVKITLGPKGRNVVLEKKF
                     GSPTITKDGVTVAKEIELPEPLENLGAQMVREVASKTSDVAGDGTTTATVLAQSIFRQ
                     GIKSVTAGSNPMALKRGIERAIETAIEEIKRLAKPVSGDAIAQVGTISANNDSTIGKI
                     IAEAMEKVGKDGVITVEESKTMETVLDVVEGMQFDRGYLSPYFVTDAERMECVLENPY
                     ILIHEKKISNMKDLLPLLEQIARSGKPLLIVAEEVEGEALATLVVNKLRGTLNCCAVK
                     APGFGDRRKAMLEDIAILTNGKAIMEETGIKLEGVKLEDLGRAKRVTVDKDNTTIVEG
                     AGSKKAIEGRVKQLRTQIEETTSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEKK
                     ARVEDAMHATRAAVEEGIVPGGGVALVRSIPAVKKLKLEGDEQTGVDIVLRALEEPLR
                     QIASNAGYEGSIVVEKVKAEKNSNFGFNAETGEYEDLVKSGVIDPAKVVRFALQNAAS
                     IAALMLTTEALVSEIPEKEPAGPPSGPPGGMY"
     gene            complement(2854..>3883)
                     /locus_tag="VFA54_05115"
     CDS             complement(2854..>3883)
                     /locus_tag="VFA54_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZS50218.1"
                     /translation="YVSSYWRNGPVLNNAISGVDQALWDIKGKRANMPVYQLLGGKSR
                     FAVDLYAHASGNSFPEVEDNIRKWMEQGYRNVRAQVAIPGQATYGVAGRGAGRAADSA
                     GPLPPAGPPRQGVFDSAPYVRTVPKLFEYLRSKLGDRVELLHDVHERVTPTEAIGLCK
                     ELEKYRLFFVEDALSPEDIQYFRIIRQQCSTPLAMGELFNSPHEFMGLISERLIDYIR
                     IHISQIGGLTPARKVAALAEWFNVRTAWHGPGDVSPVGHCANIALDISCRNFGIQEQT
                     VFPKETQEVFPGCPKIKDGYYWVNEAPGWGIDLDEKLAAKFPPKADTPFDYNWGSTRK
                     SDGTIIRP"
BASE COUNT          851 a         1070 c         1240 g          722 t
ORIGIN      
        1 tgactcggct caagtgattc tccaccagtc gctgcgcgag caatacgaat ggatggcgag
       61 ctacacgcgg tcccgggcct attccaacgc cgtcctcgac atcacgatcg atcagccgat
      121 gctgatttcg agcaacgtgg gtcccatgcc gtgggatagc cccaaccggt ttctgagttg
      181 gggttacctg ccgacgccgc acaagaattg ggccctcgct tatctgctcg aggtgcgcga
      241 cgggttcccg ttttccctag tgaaccaaca gggtcaggtc atcggcggcg tcaatgcgtc
      301 ccgcttgccg atgtacttta atctcaacct gcatctggag cgccgcttcc gtctcggcag
      361 ttaccatttc gccttgcggg gcggctttaa caacgccacc aaccacagga atcctaccgt
      421 cgtcaacaac acgctcggct cgcctcagtt tttgacttat tacgggagcg aagggcggca
      481 ctttgtgttc cggctgcgct ggctgggaaa agattagccc acagcggggc ggaatccttt
      541 ccagtcgttt tcggcaatcc ggctcgccgc aggcgcttga catggatggc ggcaggtcgt
      601 atcattagca ctctaaggca gagagtgcta gaaggggcgc ggcccctttc tcggtccagt
      661 caaaaatcaa gataactttc ggtacagaaa ggagatagcg atgaaatttc gacctctaca
      721 tgatcgtgtt ctcgtgcgcc gtatagagga agcggagacg gtccggggcg gcatcatcat
      781 ccccgatacg gctaaggaaa aaccacagga aggcgaggcc attgcctgtg gcaatggaaa
      841 actgctggac accgggcaac gcgtggcgct ggaggtgaaa ccgggcgatc gcgtgctgtt
      901 tggcaaatac tcagggaatg aaatcaaact ggacggcgag gactttctga ttttacgcga
      961 agacgagatc cttgcgattc tcgacaaaaa ttcatcggga tccgacaaca agaaagggaa
     1021 aaaatagagg gagacatagc gcatgcccgc aaaaaaaatc attttaggcg aggattcgcg
     1081 gcaatcgatt cttcgcggcg taaacgccct ggccgacaca gtaaaaatta ctctcggccc
     1141 taaggggcgg aatgtggttc tggagaagaa atttggctcg ccgacgatca cgaaagacgg
     1201 cgtgactgtg gctaaagaaa tcgagctgcc cgagccgctg gagaatttgg gcgcccaaat
     1261 ggtgcgcgaa gtcgcatcca aaaccagcga tgtagccgga gacggcacta ctacggccac
     1321 cgtgctggcg cagtcgatct tccgccaggg aatcaagagc gtcacggccg ggtccaaccc
     1381 gatggcgctc aaacgaggga tcgaaagagc gatcgagacg gccattgaag aaatcaagcg
     1441 gctggcgaag ccggtctcgg gtgacgccat cgcgcaggtc gggacgatct cggccaacaa
     1501 cgactcgacc atcgggaaaa tcattgccga ggctatggag aaggtcggca aggacggcgt
     1561 gatcacggtc gaagagtcca aaaccatgga gacggtgctg gatgtggtcg aagggatgca
     1621 gttcgaccgc ggctacctgt cgccctattt cgttacggac gccgagcgta tggagtgcgt
     1681 gctcgaaaat ccgtacatcc tgattcacga gaagaagatc agcaacatga aggatctgct
     1741 gccgctgctg gagcagatcg cgcggtccgg caagccgctg ctgatcgtgg cggaagaagt
     1801 ggaaggcgaa gcgctggcca cgctggtggt gaacaagctg cgggggacgc taaactgctg
     1861 cgcggtgaag gcgcccggtt tcggcgatcg ccggaaagcg atgctggagg atatcgcgat
     1921 cctgaccaac ggcaaggcga tcatggagga gaccggcatc aagctcgaag gcgtgaagct
     1981 ggaggacctc ggccgggcca agcgggtgac ggtagacaag gacaacacga cgatcgtcga
     2041 aggcgccgga tcgaagaagg cgatcgaggg gcgcgtgaag cagttgcgga cccagatcga
     2101 ggagaccacg tcggactacg accgggagaa actgcaagag cggctggcga agctggtggg
     2161 cggcgtggcg gtgatcaagg taggcgcggc gaccgagacg gagatgaagg agaagaaggc
     2221 gcgggtggaa gacgccatgc acgccacgcg ggcggcagta gaggaaggta ttgtgccggg
     2281 cggcggcgtg gctctggtgc gttccatccc ggcggtgaag aagctgaagc tcgaaggaga
     2341 tgagcagacc ggcgtagata tcgtcctccg ggctttggaa gagccgctgc ggcagatcgc
     2401 atccaatgcc ggctacgaag gctcgatcgt ggtggagaaa gtcaaggcgg aaaagaattc
     2461 caattttggg ttcaatgcgg aaaccggcga atacgaggac ttggtgaaat ccggggtgat
     2521 cgacccggcg aaggttgtcc gcttcgcctt gcagaacgct gcttccattg ctgcgctcat
     2581 gctgacgacg gaagcgctgg tcagcgagat tccagaaaaa gagcctgccg gacctccttc
     2641 ggggcctccc ggcggcatgt actgaccgac taacccagca gctgggaagc gccagggcgt
     2701 agagtcctgg cgcttttttc gtggagcgct gtctggaaac tgcggcctgt ggccgcgaat
     2761 caggcgcagt cggtcccgca tccaggccac gaagtggtcc tccccaaaaa acgggactgg
     2821 tccgcgagat ggaacccacg gggcgtagag cgcttagggc cgaatgatcg tcccgtcgct
     2881 ttttcgggta ctgccccagt tgtagtcaaa gggagtatcg gctttcggcg ggaattttgc
     2941 cgcgagcttc tcatccagat cgatgcccca tcccggcgct tcattgaccc agtagtagcc
     3001 gtctttgatt ttcgggcagc ccgggaagac ctcttgcgtc tctttcggga agacggtctg
     3061 ctcctggatg ccgaaattgc ggcaggaaat atcgagcgcg atgttggcgc aatggccgac
     3121 cggagagacg tcccccggcc cgtgccaggc tgtccgcacg ttgaaccatt cggccagagc
     3181 cgcaaccttg cgcgcgggcg tcaggccgcc gatctgggaa atgtgaattc gtatgtagtc
     3241 gatcagccgc tcggaaatca accccataaa ttcgtgcggg ctattgaaga gttctcccat
     3301 cgccagcggc gtcgagcact gctggcggat aattcggaag tattggatgt cttccggcga
     3361 aagagcgtcc tccacaaaga acaggcggta tttttccagt tctttgcaaa ggccgatagc
     3421 ctcggtgggc gtcacgcgct catggacgtc gtgcagcagt tcgacccggt cgcccagttt
     3481 gcttcgcagg tactcaaaca gcttgggaac agtgcggaca taaggagcgg aatcaaacac
     3541 gccctgccgc gggggcccag ccggcggaag cgggccggcg ctgtctgccg ctctgcctgc
     3601 gccgcgcccg gccacgccgt acgtggcctg gcccggaatc gccacttgcg cccgtacatt
     3661 cctgtatccc tgctccatcc acttccggat gttgtcttcc acctccggga agctgttgcc
     3721 gctggcgtgc gcgtacagat ccacggcgaa ccgcgatttg cctcccaata gttggtatac
     3781 aggcatgttg gcccgcttgc ccttgatgtc ccacagcgcc tggtctacgc cgctgatagc
     3841 gttgttgagc acggggccgt tgcgccagta agagctgacg tag
//
LOCUS       DASREK010000101         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_222629, whole genome shotgun sequence.
ACCESSION   DASREK010000101 DASREK010000000
VERSION     DASREK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222629"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VFA54_05120"
     CDS             <1..1363
                     /locus_tag="VFA54_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZS50219.1"
                     /translation="PMYQTLPAVIRRIRRGGGPALVEGQVIRMDAHSSSDDQRKYRAE
                     EELQELLVRDPIYQTERYLYRNKILTPEQTDALRAEVKAEVDRAADEADRHPMPSADN
                     LLAHIYSDDPAVGAEPVPPRYISSEPVTMLDAINHGLREELEQNPKIVMWGEDIADPK
                     GGVFGVTRGLSTAFPDRVSNSPLAEASIVGVAGGMAIGGYKPIVEIQFGDYLWPGFMQ
                     LRNEIPTVRWRSQGVWTCPVVVRIAVGGYIKGGPWHSACVEAFFARIPGWYVVFPSCA
                     DDAKGLIKSAARSQDPVIFLEHKGLYRRIQAKTPEPDADYLVPFGRGRIRREGTDITI
                     VTWGSTVYLALELARQMETEGRSLEIIDLRSIVPFDEQIVYDSIRKTNRVVVAHEDTF
                     TMGFGAEVAARIAANCFEHLDAPVKRVAAQDSFVPSASNLELMVLPSVEDLRRGVDEV
                     LHY"
     gene            complement(1491..1901)
                     /locus_tag="VFA54_05125"
     CDS             complement(1491..1901)
                     /locus_tag="VFA54_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HZS50220.1"
                     /translation="MQRPIRVLIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQTP
                     EMIVSAALQEDVDFIGLSILSGAHNAIVARLMALLKQHGMEDVKVIVGGIIPDADIAA
                     LKQLGVSAIFQPGTSMEEIVRFIRENAPARDSIA"
     gene            complement(2022..3605)
                     /locus_tag="VFA54_05130"
     CDS             complement(2022..3605)
                     /locus_tag="VFA54_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZS50221.1"
                     /translation="MPGEFTTISGTPVQRLYTPADLPDFDPATDLGMPGEFPFTRGIH
                     RDMYRGKLWTMRQFSGFATPEETNRRYRYLLEQGQTGLSVAFDLPTLMGYDADDEMAI
                     GEVGKCGVSISSLADMEVLFEGIPLGCISTSMTISSPAPMLWAMYLVVAEKQGAKWET
                     LSGTIQNDILKEYIAQKEYIYPPRPSMRLVVDTFEFGSRRAPKYNTISISGYHIREAG
                     STALQELAFTLRDGIEYVLWGIERGLDVDEFAPRLSFFFDAHNDFFEEIAKFRAARKI
                     WAHVMRERFHARNERSWKLRFHTQTAGCSLTAQQPRVNIVRTALQALAAVLGGTQSLH
                     TNALDEAWALPTEEAALLALRTQQVIAHESGVVDTADPLGGSYFLERLTLDLEKGCYQ
                     YFDRIDSMGGMIAAIERGFPMREIAEASYRYQQAVEKKQKIVVGVNAFEAGAEPIETL
                     YIDRSVEEQQIARLRKLRAERDQARVRRALDDLSRAAEREQNTMPYLIEAVRSYATLG
                     EMCGALKKVFGVYREAAVT"
     gene            4010..>4223
                     /locus_tag="VFA54_05135"
     CDS             4010..>4223
                     /locus_tag="VFA54_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50222.1"
                     /translation="MRTFPLLSALTVCVLTGQGWSQPSQAPARADGRGDRIRQMPPQA
                     NLGGAGTTGNGIDYHGGPVLDTVLGSP"
BASE COUNT          810 a         1309 c         1278 g          826 t
ORIGIN      
        1 gccgatgtac cagacgctgc cggccgtaat ccgccgtatc cggaggggag gcggacccgc
       61 tctggtcgaa gggcaagtca tccgcatgga tgcgcattcc tcctcggatg accagcggaa
      121 atatcgcgcg gaggaggaac tgcaagagct gctggtccgc gacccgattt atcagacgga
      181 gcgctacctg tatcgcaaca agattctcac ccccgaacag accgacgcgc tgcgcgctga
      241 agtgaaggcg gaagtagacc gagcggccga tgaggcggac cggcatccga tgccctcggc
      301 ggacaatctg ctggcccaca tttactcgga tgacccggcg gtgggggccg agccggtccc
      361 gcctcgctac atttccagcg agcccgtgac catgcttgac gcgatcaatc acggcctgcg
      421 agaggagctg gagcaaaatc ccaaaatcgt catgtggggc gaggacatcg ccgatccgaa
      481 aggcggcgtc ttcggcgtga cccgcggcct gagcaccgcc tttccggatc gcgtctccaa
      541 ctcgcctctc gcggaagcca gcatcgtagg cgtcgccggc ggtatggcca tcggcggcta
      601 caagccgatc gtcgaaatcc agttcggcga ttatctctgg cccggcttca tgcaattgcg
      661 caatgagatt cccactgtgc ggtggcggag ccagggtgtt tggacctgcc cggtagtggt
      721 gcgcatcgcc gtcggcgggt atatcaaagg cgggccgtgg cattcggcct gcgtcgaggc
      781 tttcttcgcc cggattcccg gctggtacgt ggtgtttcca agctgcgccg acgatgccaa
      841 gggcctcatc aagagcgccg cgcggtccca ggatccggtc attttcctcg agcataaggg
      901 actctaccgg cgcattcagg cgaaaacgcc ggagcccgac gcggattatc tggtcccgtt
      961 tggccgcggg cgcatccggc gcgaaggtac ggacattaca atcgtcacct ggggcagcac
     1021 ggtctatctg gccttggaac tggcccggca gatggaaaca gaaggccgat ccttagagat
     1081 tatcgacctt cgctccatcg ttccatttga cgaacaaatc gtttacgaca gcatccgcaa
     1141 aaccaaccgg gtcgttgtgg cgcacgaaga cacattcact atgggttttg gagccgaagt
     1201 ggcagcgcgc atcgcggcga actgtttcga gcatctcgat gcgccggtca agcgggtcgc
     1261 ggcccaggac tccttcgtcc cttcagcgag caacctggag ttgatggttc tcccctcggt
     1321 tgaggatctg cggcgcggcg ttgatgaggt gctgcattac tagcgggcgc ggcgtccgtt
     1381 ggatctaccg cgggccgtat tatttcgtac atgcccgcgc tcgattcttc agaggcggag
     1441 gctggaggca gcgtttcgtc ccgggtgaag cgggagattg tcctgcttgc ctacgcgatg
     1501 gaatcccggg ctggcgcatt ttcccggatg aaccgcacga tctcttccat ggaagttccc
     1561 ggctggaaaa tggcgcttac ccccagttgc ttcagagcag caatatccgc atcagggata
     1621 atcccgccga caatcacctt cacatcctcc atgccgtgct gttttagcag cgccatcaaa
     1681 cgagccacga tggcgttgtg cgcgccggaa aggatggaga ggccgataaa atcgacatcc
     1741 tcctgaaggg ccgccgagac gatcatttcc ggcgtttggc gcaggccggt gtaaatcacc
     1801 tccatgccgg cgtcgcgcaa ggcccgggcg attaccttgg cgccgcggtc atggccatcg
     1861 agtccgggtt tggcgatcaa cacacgaatg ggacgctgca tcttctctcg agacacccca
     1921 aggaaacgta gagcaaagcg gctcccgcca aatagttgct atcggaaaca gcatgacgcc
     1981 gccgttcggg tccgcgccgg ggaggtgcat ccgcaacccg tttaagtgac ggccgcctca
     2041 cggtagacgc cgaaaacctt cttcagggcg ccgcacattt ctcccagggt agcgtagctg
     2101 cgcacggctt ctatgaggta gggcatcgta ttctgctccc gctcggcggc ccggctcaga
     2161 tcgtccaatg cgcgccgcac gcgggcttgg tcgcgctcgg cgcggagctt ccgcaggcgc
     2221 gcaatctgct gttcctccac gctccgatcg atgtacagcg tctcgatcgg ttcggccccc
     2281 gcctcgaagg cgttgacgcc aactacgatt ttttgcttct tttcgacggc ctgttgatac
     2341 cggtagctgg cttccgcgat ttcgcgcatc gggaagccgc gctcgatcgc cgcgatcatg
     2401 ccgcccatgg aatcgatgcg gtcgaaatac tgataacagc ctttctccag atcgagcgtc
     2461 agccgctcca ggaaatagct gccgcccagc ggatcagccg tgtccacaac gccgctctcg
     2521 tgggcgatca cctgctgcgt gcggagcgcc agcagcgccg cctcctcggt ggggagcgcc
     2581 caggcctcgt caagcgcatt agtgtgaaga gactgcgtgc cgccgagcac cgccgccagc
     2641 gcttgcagcg ccgtgcggac aatgttgacc ctgggttgtt gcgcagtcag ggaacagccg
     2701 gcggtctggg tatgaaagcg cagcttccag gaacgctcgt tcctggcgtg gaaacgttcg
     2761 cgcatcacgt gcgcccatat ttttcgcgcc gcgcggaact tggcgatctc ttcgaagaaa
     2821 tcgttgtggg cgtcgaaaaa gaagctgagg cgcggggcga attcatccac atccagcccc
     2881 cgctcgatgc cccacagcac atactcgatg ccgtcgcgca gcgtgaacgc cagttcctgg
     2941 agagccgtcg atcccgcttc gcggatatga tagccgctga tggaaatcgt attgtacttg
     3001 ggcgcgcgac gcgatccgaa ctcgaaggta tccacaacga gccgcatcga cggccgcggc
     3061 ggataaatat actccttttg cgctatgtac tccttgagaa tgtcgttttg gatcgtgccg
     3121 gagagagtct cccatttcgc cccttgcttt tcggccacta ccagatacat cgcccagagc
     3181 atgggcgccg gggaagaaat agtcatcgaa gtcgagatgc acccgagcgg gataccttcg
     3241 aacagcactt ccatgtcggc gagcgatgaa atcgacacgc cgcatttccc cacttctccg
     3301 atcgccattt catcatcggc gtcgtatccc atcagcgtgg gaagatcgaa agccacggat
     3361 aggccggtct ggccttgctc cagcaggtac ctgtagcggc gattggtttc ctccggcgtg
     3421 gcaaagccgg agaactggcg catcgtccaa agctttccgc gatacatgtc ccggtgtata
     3481 ccccgggtaa atggaaactc tccaggcatt ccgaggtcag tggcagggtc gaaatccgga
     3541 agatctgccg gcgtgtaaag tctctggacg ggtgttccgc tgatcgtagt gaactcgccg
     3601 ggcatacagg ccgcctgctt tcaatataca ggatcagggg gccgctttct gggccgcctc
     3661 gacgccgccc ttagcccttt ttctttaact tagagcgcga gcgcataaaa accgaatcgg
     3721 cgcttcccag atgaagtttc caggcgcgcg cgcgcacggc ggctccgcag acgggatggt
     3781 tttccgcgcc tgtgccaccc tgcgccgggc aagggccggg ggagcctttc cggggcggct
     3841 atgctgccag cggggagggc cgggccgttc tcacgcgctt tccatcagaa ataaatatct
     3901 tgccttacaa ggacttagcc gcaaagtact attgaccact aatcagggcg tatgaaaaac
     3961 tgtgcgctgc catacccatc cgtcgcagct cgccggcgcc ggaggcccca tgcgaacttt
     4021 cccgctgctc agcgctctca cagtatgtgt tctgacgggc caaggctgga gccagccgtc
     4081 acaagccccg gctcgcgccg atggcagagg cgaccgcatc cggcaaatgc cgccgcaagc
     4141 caatttgggc ggcgcgggta cgacaggaaa tggaattgac taccacggag gccccgttct
     4201 cgatacggtt ctcggttctc cga
//
LOCUS       DASREK010000102        14657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1045270, whole genome shotgun sequence.
ACCESSION   DASREK010000102 DASREK010000000
VERSION     DASREK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 14657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14657
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045270"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFA54_05140"
     CDS             <1..328
                     /locus_tag="VFA54_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50223.1"
                     /translation="IVGAGKIPTPVDEAEIRAIQTIIKSGLPAEPWPFLRVGQLVRIE
                     AGPLADLEGILLDIKNRRRLIVSVTILQRSVAVEIERFWVTPIRGLRRPAASTASAFV
                     PDVKSA"
     gene            390..749
                     /locus_tag="VFA54_05145"
     CDS             390..749
                     /locus_tag="VFA54_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50224.1"
                     /translation="MTVLKRRTRMVNFRLSEDEYKELKNLCIANGSRSISDFARAAVC
                     QSLGGNGNAEKFEGTVRELHGKFAELDREVKRLAQLFAEAGSPAVPKKNAASSPGESG
                     AASRKVINGDKLCGSVS"
     gene            731..1783
                     /locus_tag="VFA54_05150"
     CDS             731..1783
                     /locus_tag="VFA54_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZS50225.1"
                     /translation="MRISLLILGMTWLAAAASGAPQREVTSAPPVAPVSPSVHPQESE
                     TKTVEPRPATEEAPSSYVLGPGDQIVIRAFDAEEISDKPTRISSSGYINLPLVGRVRA
                     AGMTVEQLEADLTSRLQTYIQSPEVSVSVSEFRSQPVSVIGAVNAPGVHQLQGHKTLI
                     EILSLAGGTRTDAGHTVKITRRLEWGRIPLPNAKDDPTGQFSVAEVNLKSVLDARNPQ
                     DNILIQPEDVISVPRAEMVYVVGDVPRAGGFVLNEQESVTVLQALSLAGGLGRAAAPT
                     KAKLLRAGGKGASRTELAVNLKDILAGKKDDVPMQPEDILFIPNSASKSVALRSLETA
                     LGMGAQIGAGIAIYSH"
     gene            1803..4244
                     /locus_tag="VFA54_05155"
     CDS             1803..4244
                     /locus_tag="VFA54_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZS50226.1"
                     /translation="MENSNLIPSDRDPAEGPSGDHGNLHYDVARLEARIEELCQSARE
                     HMPGRLQLVEAGDPELADSEISSSLLVYWHMLRRHKSTIVIIAFLGAVAGVLFSLPQT
                     PIYQAQTSLEIQGLNENFLNMTNMNPTTTSGFYPEYDIQTQMKILQSRTLIERAVTKL
                     KGEESADPPPPLGRLAAWRKALGLGTGPSMDPRQQALVTAASTVRVRNTGQNRIVDIT
                     CDSTDPKMAADFANTLASEFIEQNLESRWNSSQKTGEWLTRQLQDLKIKLEKSEDQLQ
                     SYARASGLMFTSEKDNENVDQEKLKQLQQELSKAQADRVSKQSLYEMALSSPPDALPE
                     VLDSESLAEYQMKLVDLKRQLADLTSSFTPAHPKVKRLQAQIAEIEASIKKERGNIMT
                     RIRNEYDSAQRRERLLATAYATQAKIVSEQAEKAIHYNILKREVDTNRQLYDAMLQKV
                     KEAGITSAMRASNVRVVDPARPPGGPYKPNPSQNAALGLLTGVFLGIVFVLVREHADR
                     SLRAPGEASTYLNVPELGVIPSTRADSGKAPSSRDLTLRIGSPAGDNSGPNGTEDGAG
                     ARVELITWQQKPSLLAESFRATLASILFSGQNGNRPRVLVVTSPSPSEGKTTVISNLA
                     IALAEISRRVLLIDADMRKPRLHDVFDVPNSWGLSDLLRERNPIDDCPREALARETAI
                     PGLYLLPSGPGTVSISNLLYSARVPELLRRFRREFDTVLIDTPPMLQISDARVLGRLA
                     DAVILVLRAGQTTRDSAIAAKQRFAEDGTLVLGTVLNYWNPAASTNGYGYGYGYGYGY
                     GYYDAYHSHYAGKGS"
     gene            4250..5293
                     /locus_tag="VFA54_05160"
     CDS             4250..5293
                     /locus_tag="VFA54_05160"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZS50227.1"
                     /translation="MAHYLVTGAAGFIGAAVVEQLLAGGHHVTGIDNLNDAYDVRLKE
                     WRLRQLLPYPRFVFQRLDICDWKKLSEFWQASPPFDAVLNLAARAGVRQSVENPWLYT
                     ETNVTGTLNLLELSKIHGVRKFILASTSSLYGSHNPMPYAEDADTSRPLSPYAASKKA
                     AEALCYTYHYLHGLDATVLRYFTVYGPAGRPDMSPFRFVQWISEGRPLVVYGDGRQSR
                     DFTYVADIARGTIASLALRGYSTINLGSDRPAPILDLIRMIEQQLKQKARLEFHPPHA
                     SDVPATWADITRARRCLGWEPTVGLEEGIASLVDWYRKNRDWAREIETFAGAPAATAE
                     RRALHPEPSPAAQ"
     gene            5313..6188
                     /locus_tag="VFA54_05165"
     CDS             5313..6188
                     /locus_tag="VFA54_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS50228.1"
                     /translation="MSANANISAELGAKAPAGATPVLRIEPTNGWVSLKLGELWEYRE
                     LLYFLIWRDIKVRYKQTALGVAWAILQPVLTMMVFSLFFGRLAKMPSDKVPYPIFTFT
                     GLVPWMFFATGLSQASNSLVGSANLITKVYFPRLAIPIATVLSGVVDFVLAFVVLLAM
                     MLYYGRLPTIHILWLPLFLLLALVTSLGVALWLSALNVEYRDVRYVIPFLTQFWMFAT
                     PIAYPSSLLYRPWRKLYALNPMVGVIEGFRWALLNTETRPGPMIVVSSLAALAILAAG
                     AFYFRRMEKTFADVV"
     gene            6172..7200
                     /locus_tag="VFA54_05170"
     CDS             6172..7200
                     /locus_tag="VFA54_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HZS50229.1"
                     /translation="MRTWCDSGGFRCKVIAEVAQNHDGSLGTAHAYIDAIAKTGADAV
                     KFQTHIASAESTPQEPWRVKFSRQDATRYDYWKRMEFTEEQWRGLAEHAGERGLIFLS
                     SAFSMEAVDLLERVGVPAWKVGAGEIGNLPMIRRMASTGKPVLLSSGLSGWDEIGAAV
                     ACVRREGAPVIVLQCTTAYPCPPEKVGLNVLGEIRERFHCPAGLSDHSGTIFAGLAAA
                     ALGAEVIEVHVAFSRECFGPDVPASITTAELAELVAGVRFIEKAVNSPVDKEAAASAL
                     EGLRQIFSKSIVAARDLPAGQLLTEGDLALRKPGTGIPPARLGQVVNRKLKRAVGANT
                     LIAEADLE"
     gene            7193..8386
                     /gene="neuC"
                     /locus_tag="VFA54_05175"
     CDS             7193..8386
                     /gene="neuC"
                     /locus_tag="VFA54_05175"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HZS50230.1"
                     /translation="MSSAPRRKVCVVLVDRANYGRLKPVMQAIAERPELELQVIAAGT
                     MVLERFGFPIDVLKRDGFRVDGEIYIELEGSTPATMAKSVGFGVVEFASEFQRLKPDI
                     VLLIGDRYEALAAAIAAAYMNLCIAHIQGGEVSGSIDESARHAITKFAHYHFPSTRQA
                     AEYLVRMGERPEAILAIGCPSSDIARTLRRHLDPAVVNRAGSGACIDVEKDFLLVVFH
                     PTTTEYGGERAQMQQVLESLKRLQIQTILLWPNIDAGSDHISKAIRTFRDMEKPAWLR
                     TIINLTPEDYLKVLANAACALGNSSSFVRDASYFGTPVVLIGSRQDGRECDVHVTRVP
                     PLADEITTAAWAQIAHGRYPPSTLYGDGRVSERIAAALVRLEPYLQKRLYYVEEPLPE
                     TVGAD"
     gene            8464..9177
                     /locus_tag="VFA54_05180"
     CDS             8464..9177
                     /locus_tag="VFA54_05180"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HZS50231.1"
                     /translation="MSGLDFRVLGVIPARGGSKGIPRKNIRLLHGRPLLSYTAEAALS
                     ARLLSRVILSTDDEEVAEVGRRCGLEVPFLRPPELATDGAPTLPVVQHAVRALAQNGG
                     RFDAVCLLQPTNPLRRPEHIDGCIELLVNSGADSVITVLPVPPEYNPHWVYFAESDGT
                     LRLSTGEDAPISRRQDLPPAFHREGSVYVIRTEVIIDGNSLYGRRIVGYPADPKASVN
                     IDVPEDWERAEELLAAGVR"
     gene            9174..9896
                     /locus_tag="VFA54_05185"
     CDS             9174..9896
                     /locus_tag="VFA54_05185"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS50232.1"
                     /translation="MTSFELLYICAEPLMSPLYFLVRRRLQAIIRECGGQASILDVGG
                     RKSHYTAGVPAAVYITDLPRKTAVQQKLHLGVNSRIVGQIRQRRSNVRGFVYDDMTHS
                     ALRERAFGCVVAVEVLEHVEDDVCFIREVHRVLRGGGVFLMTTPNGDAVQNTNPDHKR
                     HYTRAQLQSLLASHFEAVRVEYALAGGQFRRWGLQSWSVQHPVQTMRSAVGNLVTLIQ
                     SSAKHIRERPNGTRHLLAEARK"
     gene            9901..11643
                     /gene="asnB"
                     /locus_tag="VFA54_05190"
     CDS             9901..11643
                     /gene="asnB"
                     /locus_tag="VFA54_05190"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZS50233.1"
                     /translation="MCGIAGIFGVGWNEAQLGAMLASQRHRGPDDEGCYISPGGLAGL
                     AHNRLSILDLSPAGRQPMSNRDGSLWIAFNGEIYNYLELRTELRGYPYRSNTDTEVIL
                     AAYERWGRACLEHFIGMFAFLLWDEREQTLFAARDRFGVKPLYYHRQRGGTLLAASEI
                     KGLHAAGVPAAPDAGTWATYLARGLYDHSQRTFWDGVQSVPPGHFLTWRRAETKLTRW
                     YDLAERVPPDFDSRSAEAAAEEYLDLLQESVQLRFRADVPVGINLSGGLDSSTLLGLI
                     QRLQGAASEVRAYTFLTGDPRYDELPWVEQMLRRTRHPLVPCRLEPSDVPRLARSVQM
                     HQDEPFGGLPTLAYARLFERARADGVKVLLDGQGMDEQWAGYDYYQDVLNGKNGATIQ
                     GAADSPVRPECLAPEFLALAENWEMPRPFADNLRNCQYRDARHTKIPRALRFNDRISM
                     RASTELREPFLDHRLFELALRQPADRKIANGTRKVLLRAMARELLPEGVVEAPKRPLQ
                     TPQREWLRGPLRPWAEEQMEAAISAYGGSWLKPEKVRAAWQAFLNGESDNSFFVWQWL
                     SLAMIMDGVSAFTA"
     gene            11640..12950
                     /locus_tag="VFA54_05195"
     CDS             11640..12950
                     /locus_tag="VFA54_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide ABC transporter ATP-binding
                     protein"
                     /protein_id="HZS50234.1"
                     /translation="MSELAIRVAGLSKKYRIGGQRERYKTLRDSLADAAAAPFQRLAS
                     LMARRKNGNGCGESEIWALSNVEFEVSRGDVVGVIGHNGAGKSTLLKILSRITEPTSG
                     HAEIHGRVGSLLEVGTGFHLELTGRENIYLNGAILGMKRAEIERKFDEIIAFAGVEKF
                     VDTPVKHYSTGMHLRLAFAVAAHLEPEILLVDEVLAVGDAAFQKRCLGKIGDIAAEGR
                     TVLFVSHNLGAVKELCNMGLVLRNGLLAFRGSVAQALAFYGRTLTEAANDEHSGHATG
                     WRYIYLNGCSDGSLAVTTPEEPLQIHAGLELDQNCQGGLVFLLIEDFMGQLVVHRRAA
                     LSTMCSEPIGPGRYRVTSRLPAVWLAPGVYTVYLKLIIETFDGQYMRYLSEKIFLEVD
                     GATAGLSRALLAPPCEWSLTSWQAPPTFHQVASGLETRSLARPL"
     gene            13019..13825
                     /locus_tag="VFA54_05200"
     CDS             13019..13825
                     /locus_tag="VFA54_05200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50235.1"
                     /translation="MSAYSDTFYARQGEGSISSARRIVPLVIDLIAPKSVVDVGCGLG
                     SWIAVFMESGIRDVLGIDGPWVRKEMLHISPEHFQEADLARPLTLDRGFDLVVSLEVA
                     EHLPASAAQTFINSLTAMGPAVLFSAAIPFQGGIYHLNEQWQSYWAEMFMGREYVPVD
                     CIRPVIWNDPDIKVWYAQNTVLYIRQSALPCYPRLKSFSIASCDSLSLVHPRLYLGKM
                     EKLGRELQSTTRLGTLFAESAIRRLPGAALAARVVRSLRHSIKDSAPSGQ"
     gene            13829..>14657
                     /locus_tag="VFA54_05205"
     CDS             13829..>14657
                     /locus_tag="VFA54_05205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS50236.1"
                     /translation="MITPSVTVVMPVFNQACYARAAIDSILNQTVSKFEFLIVDDGST
                     DGTSHVLHAVTDSRIRVIRIPHSGFLTALRTGVEAASGDLVARMDSDDLCHPKRLERQ
                     IAFLEAHPECVFVTTYYGLVTPRGYFLAPKQSFDWKYVTAADITQGNGLFCDPGTIFH
                     RRQAVSAGLYDSEFEYETPLWYKLLRYGQGAVLGASLYYARWLVSSYSRDESGLRRRR
                     QVHRLIRLKYDPEGATKIPAKASPKNEPSGISVARRAIGLYLLAGDKASAREVAIKAW
                     "
BASE COUNT         2908 a         4381 c         4457 g         2911 t
ORIGIN      
        1 catcgtcggc gccggtaaga ttcccacgcc tgtagacgaa gccgaaattc gcgctattca
       61 gaccattatc aaatccggcc tccccgccga gccgtggccc tttctccgcg tgggccagtt
      121 ggttcgaatc gaggcgggac ctctcgctga tctggagggc attctgctgg acatcaaaaa
      181 ccggcgccgg ctcattgtct cggtgaccat tcttcaaagg tccgtcgcag tagaaatcga
      241 gcgtttctgg gtaacgccga ttcgagggct ccgccgccct gccgccagca ctgcttcggc
      301 cttcgtcccg gacgtcaagt ccgcttagaa tcccgctttc tcaaccggtc gtttggttat
      361 taggcgggac agccgcagga gcacctagca tgactgtact aaagcgtaga acgcgaatgg
      421 tcaattttcg tctctccgaa gacgaataca aggaactgaa gaatctctgc atcgccaacg
      481 gatccagaag catttccgac tttgcgcggg ccgccgtatg ccagtcgctc gggggcaatg
      541 gcaatgcgga gaaattcgaa ggcacagtgc gagagctgca tgggaaattc gccgagcttg
      601 accgcgaggt caagagatta gcgcagctgt tcgcagaggc agggtctccc gcagtcccca
      661 agaagaacgc cgcctccagc cccggcgaaa gcggcgccgc ttcgcgcaaa gtaatcaatg
      721 gagataagtt atgcggatca gtctcttgat tctaggtatg acatggctgg cagcggccgc
      781 ttcgggcgcg ccgcagcgag aggttacatc cgcgccgcca gtcgcccccg tgtccccaag
      841 cgtccatccg caggagagcg aaaccaagac tgtggagcct cgtccggcga cggaagaagc
      901 gccttcctcg tacgttctcg gtccgggcga ccagatcgtc atccgcgcgt tcgatgcaga
      961 agaaatttcc gacaaaccga ctcgaatctc gagcagcggt tacatcaatc tgccgctggt
     1021 gggccgtgtg cgcgccgccg ggatgacggt cgagcagctc gaagccgatc taacgtcacg
     1081 gctgcaaacc tacattcaat ctcctgaggt ttcggtaagc gtcagtgagt tccgcagcca
     1141 gccggtctcc gtgatcggcg ctgtcaatgc accgggtgtt caccaattgc agggccacaa
     1201 aactctgatc gagatccttt ccctggccgg gggaacgcgc actgatgcgg gacacacggt
     1261 caaaatcacc cgccggctgg aatggggacg gattccactg ccgaacgcca aagacgaccc
     1321 caccgggcag ttcagcgttg ccgaagtcaa tttgaaatcg gtcctggacg ccagaaaccc
     1381 gcaggacaac attctgatcc aacccgagga cgtcatctcg gtgccgcgcg ccgagatggt
     1441 ctatgtggtc ggcgacgtgc ccagggccgg cggctttgtt ctcaatgagc aagaaagcgt
     1501 taccgtgctg caagctctct cgctggccgg cggcctgggg cgcgctgccg ctcctaccaa
     1561 agccaagctg ttgcgcgcag ggggcaaagg ggccagccgg actgagttgg ctgtgaacct
     1621 gaaagacatt ctggcgggaa aaaaggacga cgttcccatg cagccggaag acatcttgtt
     1681 tatcccgaac agcgcgagca agagcgttgc tctccggtcc ttggaaacgg cgctgggcat
     1741 gggagcgcag atcggcgcgg gcatcgcgat ttacagccat tgagctggaa gaaggagcgg
     1801 ccatggaaaa ctcaaatttg attcccagcg atcgagatcc cgcagaaggc ccctccggcg
     1861 accacgggaa tctgcattac gacgtcgcaa gactagaagc ccggatcgaa gaattgtgcc
     1921 agagcgcgag agagcacatg ccggggcgcc tgcaactggt ggaggccggc gatccggagt
     1981 tggccgattc cgaaatttcg agcagcctgc ttgtctactg gcacatgctg cgccggcaca
     2041 aaagcaccat cgtgattatc gcctttttgg gggcggtggc cggggttctt ttcagccttc
     2101 cgcagacgcc gatttatcag gcgcagacct cccttgagat ccagggcctg aacgaaaact
     2161 ttttgaacat gaccaatatg aacccgacca ctacgtcggg tttttatccc gagtacgaca
     2221 ttcagacgca gatgaagatt ctgcaaagcc gcacgctcat cgagagagcg gtcaccaagc
     2281 taaaggggga ggaatccgca gatccgccgc cgcctctggg gcggctggcc gcttggcgca
     2341 aagccttggg tctcggcaca ggcccgagta tggatccgcg ccaacaggct ttggtgacgg
     2401 ctgccagcac ggtccgggtc cggaatacag gccaaaaccg gatcgtcgat attacgtgcg
     2461 attcgacaga tcccaaaatg gcggccgatt tcgccaacac tctggccagc gaatttatcg
     2521 agcagaatct ggaatcccgc tggaactcat cccagaaaac cggagaatgg ttgacccgcc
     2581 agcttcagga cctgaagatc aaattggaaa aatccgaaga tcaattgcaa tcctacgccc
     2641 gagcgtcggg cctgatgttc acttccgaaa aggataatga aaacgtcgac caagaaaagc
     2701 taaagcaatt gcagcaagag ctctccaaag cgcaagccga cagggtgagc aagcaatccc
     2761 tctatgaaat ggctctctcc agcccgcctg acgcgctgcc cgaggtattg gacagcgagt
     2821 ccctcgcgga gtatcagatg aagctggtgg atctgaagcg ccaattagct gacctcactt
     2881 cgtccttcac gccggctcac ccgaaggtga aacgactaca ggcgcagatc gcggagatcg
     2941 aagcgtccat caagaaagag cgcggcaaca taatgactcg gatccgaaac gaatatgatt
     3001 ccgcccagcg cagggagcgc ttgctcgcaa ccgcgtacgc cacgcaggcc aagatcgttt
     3061 ccgagcaggc ggaaaaagca attcattaca acattctaaa acgtgaggtg gatactaacc
     3121 gccagcttta cgacgccatg cttcaaaagg tcaaggaggc gggtattacc tcggcgatgc
     3181 gggccagcaa cgtacgcgtg gtcgatcccg ccaggccgcc cggagggcct tataagccga
     3241 atccgagcca aaacgccgcg ctgggactgc tcaccggcgt tttcctcggg atcgtctttg
     3301 tgctggtgcg cgagcacgcg gaccggagcc tcagggcgcc cggcgaagcc tctacgtacc
     3361 tgaacgtgcc cgaactcggc gtgatcccct ctacgcgcgc tgattccggc aaagccccct
     3421 ccagccgcga tctgactcta agaatcggaa gtcctgccgg cgacaacagc ggacccaacg
     3481 gaacggagga cggcgccggc gcccgcgtcg agttgattac ctggcagcag aagccgtcgc
     3541 tgctggcgga atcgttccgg gcgacgttag cctccatcct gttttcagga cagaatggca
     3601 accgtcctcg cgtcctggtc gtgaccagcc caagcccatc ggaaggaaag acgacggtca
     3661 tcagcaacct cgcgatcgcg ctcgccgaaa tcagccgacg cgtactgctg atcgacgccg
     3721 atatgcgaaa gcctcggctg cacgatgtat ttgatgtacc caatagctgg ggtttgagcg
     3781 acctgctgcg cgagcgaaat ccgattgacg attgcccgcg agaggcgctg gcccgggaaa
     3841 ccgcgatacc gggtctgtat ctgctgccca gcgggccggg cacggttagt atctccaacc
     3901 tcctttactc ggcccgagtc ccggagctgc tgcggcggtt ccggcgtgag ttcgataccg
     3961 tgctgatcga taccccgccg atgctccaga tttccgacgc ccgagtgctc ggccgcctgg
     4021 ctgacgccgt gattctggtt cttcgcgcgg gccaaactac gcgggatagc gcaattgccg
     4081 cgaagcagcg cttcgccgag gatggaacgc tcgtgctggg aacagtgttg aactactgga
     4141 accccgcggc gtcaacgaat ggttacggtt atggctacgg ttatggttat ggctacggct
     4201 attacgatgc ctaccacagc cattatgcag gcaaaggaag ttagcggaga tggctcatta
     4261 tcttgtcact ggggccgccg ggttcatcgg cgccgcagtg gtggaacaac tcctcgcagg
     4321 cggccatcac gtcaccggca tcgataacct gaacgacgct tacgatgtgc ggctcaaaga
     4381 gtggcggctg cgccagcttt tgccttatcc aaggttcgtg ttccagcgcc tggatatttg
     4441 tgactggaaa aagctgtccg aattctggca ggccagccct ccgtttgacg ccgtgctgaa
     4501 tctggctgcc cgggcaggcg tgcggcaaag cgttgaaaat ccctggctgt acacggaaac
     4561 caacgtaacc ggcacgctga accttctcga actctcgaag attcacggcg tacgcaagtt
     4621 catcctggct tcgacttcca gcctgtatgg gtctcataat cccatgccct acgccgagga
     4681 tgcggatacc agccggccgc tttccccgta tgcggcttct aagaaggctg cggaagcgct
     4741 ttgctatacc tatcactact tgcacggcct ggacgcgaca gtgctgcggt acttcacggt
     4801 ttacggtccc gccgggcggc ccgatatgag tccctttcga ttcgtgcagt ggatcagcga
     4861 agggcgcccc ttggtggtgt acggcgacgg gcgccagtcc cgggacttca cctatgtcgc
     4921 cgacatcgcg cggggaacca tcgcttcttt ggcgctgcgt gggtactcga ccatcaacct
     4981 ggggtcggac cggccagcgc cgatcctgga tttgattcgc atgattgaac agcagctaaa
     5041 acagaaagcg cggcttgagt tccatccgcc ccacgcttct gacgttccgg ccacctgggc
     5101 cgatatcaca cgcgcgcgcc gctgccttgg ctgggaaccc acagtcgggc tggaagaagg
     5161 aatcgcatcc ctggtcgatt ggtacagaaa aaatcgagac tgggctcgcg aaattgagac
     5221 ttttgccggc gcgcccgccg ccacggcaga gcgcagggcc ttgcatcccg agccttcgcc
     5281 ggctgcgcag tagaactgga tttctctgaa ccatgagcgc gaacgccaat atttctgctg
     5341 agctcggagc caaggcgccc gccggcgcca ccccagtctt gcggatcgag cccacaaacg
     5401 gttgggtgtc tctcaagtta ggcgagcttt gggaataccg ggagctgctt tactttctga
     5461 tttggagaga tatcaaggtc cggtacaagc aaaccgcttt gggggtagcc tgggctatcc
     5521 ttcagcctgt tttgaccatg atggtgttca gcttgttttt tggccggctg gcgaagatgc
     5581 cctcggacaa ggtcccgtat ccgattttta ccttcaccgg gctcgtcccc tggatgttct
     5641 tcgccacggg cctgagccag gcttccaaca gcctggtggg cagcgcgaac cttatcacca
     5701 aagtgtattt ccccaggctg gcgataccta tcgctacggt gctttccggc gtcgtagatt
     5761 tcgtgctcgc gttcgttgtt cttctggcaa tgatgctcta ttacggacgc ctgcccacca
     5821 tacatatcct ctggctgccg cttttcctgc tgcttgccct ggttacatcc ctgggcgtcg
     5881 cgctatggct ttcggctctc aatgtggaat accgggacgt ccgttatgtg atcccctttt
     5941 taacacagtt ctggatgttc gctacgccga tcgcctatcc cagcagcctg ctctaccggc
     6001 cctggcggaa gctttacgcg ttgaatccga tggtcggcgt tatcgaaggc tttcgttggg
     6061 ctttgctgaa tacggaaacg aggcccggcc cgatgattgt ggtgtcgtct ctggcggcgc
     6121 tggccattct ggccgccggc gcgttctatt tccggcgaat ggagaaaact tttgcggacg
     6181 tggtgtgaca gcggtggttt ccgctgcaag gtcatcgccg aggtggcgca gaatcacgac
     6241 ggcagcctgg gaaccgccca cgcgtatatt gacgcgattg caaagacggg cgcggatgcg
     6301 gtcaagtttc agacgcacat cgccagcgcc gagagcacgc cgcaggaacc ctggcgcgtc
     6361 aaattcagcc gccaggacgc cacgcgctac gactattgga agcgcatgga gtttaccgag
     6421 gagcagtggc gcggcctcgc cgagcatgcc ggggagcggg ggctgatttt tctctcttcc
     6481 gctttttcta tggaggccgt ggatctgctc gagcgcgttg gcgttcccgc ctggaaagta
     6541 ggcgccggcg agattgggaa cctgcccatg attcgccgca tggcgagcac cggaaaaccg
     6601 gtactgctct cgagcggcct gtcaggctgg gacgagatcg gcgcggccgt agcctgcgtt
     6661 cgccgggaag gcgctccggt gattgtactg caatgcacta cggcgtatcc gtgcccgccg
     6721 gagaaagtcg gattgaacgt gttgggcgag attcgggagc ggtttcactg tcctgcgggc
     6781 ctgtcggacc attccggcac gatctttgct ggcctggcgg cggcggccct cggagcggag
     6841 gtcatcgaag tgcatgtggc cttttcgcgg gaatgcttcg gcccggatgt gcccgcgtcg
     6901 attactacgg ccgaattggc cgaactggtg gccggcgtgc gcttcatcga gaaagcagtc
     6961 aacagcccgg ttgacaagga ggccgccgcg tccgcgctcg agggcctgcg ccagattttc
     7021 tccaaaagca tcgtggcggc ccgcgacctg ccggcgggac aattgctcac cgaaggggac
     7081 ctggccctcc ggaagccagg caccgggatc cctccggcgc ggctggggca ggtggtcaac
     7141 cgcaagctca agcgcgccgt gggcgcgaac acactgattg ccgaggcgga ccttgagtag
     7201 cgcaccgcgc cggaaggttt gcgtcgttct ggttgaccgc gcgaattacg gacgcctgaa
     7261 gccggtgatg caggccatcg ccgagcgccc cgagttggag cttcaagtga tcgccgccgg
     7321 cactatggtg ctcgagcgct ttggtttccc catcgacgtg ctgaaacgcg atggattccg
     7381 cgtggacggc gaaatctata tcgagctgga gggctcgacg ccggccacca tggccaagtc
     7441 cgtcggcttc ggcgtcgtgg agttcgccag cgaattccag cggctcaagc cggatatcgt
     7501 cctgctgatc ggcgaccgct acgaggcgct ggccgccgcg atcgccgcgg cctacatgaa
     7561 tctttgtatt gcccatatcc agggaggcga ggtatcgggc tccatcgatg agagcgcgcg
     7621 gcacgccatc accaagttcg cgcactatca tttcccgagc acccggcaag cggcggagta
     7681 tctggttcgc atgggggagc ggccggaagc gattctcgcg atcggttgtc cgagcagcga
     7741 catcgcgcgc acgctgcgcc gccatctcga cccggcggtg gtcaaccgcg ccggcagcgg
     7801 agcctgtatc gacgtcgaga aagactttct gctcgttgtt tttcatccga ctaccaccga
     7861 atacgggggc gagcgggcgc aaatgcagca ggtgctggaa tcgctgaagc gccttcagat
     7921 ccaaaccatc cttttgtggc cgaacatcga cgccggctcc gaccacatca gcaaggccat
     7981 ccgcaccttc cgggatatgg aaaaaccggc ctggctgcgt accattatca atcttacgcc
     8041 ggaggactat ctgaaagttc tggccaatgc cgcctgcgct ctaggaaatt ccagcagctt
     8101 cgttcgcgac gccagttact ttggtacgcc tgtcgtgctg atcggcagcc ggcaggacgg
     8161 ccgcgagtgc gatgtccacg tcacccgcgt cccgcccctg gccgatgaaa ttaccacggc
     8221 cgcctgggcg caaatcgcgc atggacgata tccgccgagc acgctctacg gagatgggcg
     8281 ggtttccgag cgcatcgcag cggccctggt ccggctggag ccttaccttc aaaagcggct
     8341 gtactacgtt gaagagccgt tgcccgagac cgtgggggcg gattgaacct ggagcgaacc
     8401 ggccgcaccc gatgccatgg agttctcgaa tcctcaaaaa caggcttttg agagggcgcc
     8461 cgcatgagcg ggctggattt ccgggtgctc ggcgtgatcc ccgcgcgcgg cggctcgaaa
     8521 ggaattccga gaaagaacat ccggctgctc catgggcgcc cgcttttgag ctatacggcg
     8581 gaggcggccc tctcggcgcg gctgctctcg cgcgtcattc tgagcaccga tgacgaagag
     8641 gttgcggaag tgggccgccg ctgcggcctg gaggtccctt ttctgcggcc cccggaattg
     8701 gccactgatg gggcgcctac gcttccggtg gtgcagcatg cagtccgcgc gcttgcgcag
     8761 aacggcggcc gttttgacgc cgtgtgtctg ctccagccga ccaatccgct gcggcggccg
     8821 gaacacatcg acggctgcat cgagctgctg gtcaacagcg gcgcagattc cgtaatcacc
     8881 gtgcttcctg tgccgccaga atacaacccg cactgggttt acttcgccga aagcgacggg
     8941 acccttcgct tgagcaccgg cgaggacgcg cccatttcgc ggcgccagga tctgccgccg
     9001 gccttccacc gcgaaggatc ggtctacgtg atccgaacag aggtaattat tgacggcaac
     9061 agcctgtacg gccgccgcat cgtgggctat ccggccgatc caaaggccag cgtgaatatt
     9121 gatgtcccgg aagattggga acgggcggag gaattgctcg ccgctggcgt aagatgacct
     9181 ctttcgagct gctctatatt tgcgcggagc cgctgatgag ccccctgtac ttcctggtgc
     9241 gcagaaggct gcaagccata atccgagaat gtggcgggca ggcctccatt ctagacgtcg
     9301 gcgggcgaaa gtctcattac acggcaggag ttccagcagc cgtctacatc actgatctgc
     9361 ccaggaaaac tgccgttcaa cagaaactcc acttgggagt taactctcga attgtgggac
     9421 agatccggca aagaagaagc aatgtgcggg gctttgttta cgatgacatg acgcactccg
     9481 cgcttagaga aagggcgttt ggctgcgtcg tcgccgtgga agttctcgaa catgtggagg
     9541 acgacgtttg tttcatccgg gaagtgcacc gggtgctgcg gggcggcggc gtctttttga
     9601 tgacgacgcc caacggcgat gccgtgcaga acaccaaccc ggatcataag cgccattaca
     9661 caagagcgca actgcaaagc ctgctggcct cgcatttcga ggcggtccgc gtggaatatg
     9721 cccttgccgg cggacaattc cggcgctggg gtctgcaatc gtggtcggtt cagcatcccg
     9781 ttcagacaat gcgtagcgcg gtgggaaatc tcgtcactct aattcagtca agcgccaaac
     9841 atataagaga gcggccgaat ggcacgcggc atttgttggc ggaagcaagg aagtgaaaac
     9901 atgtgcggca tcgctgggat cttcggtgtg ggttggaacg aggcgcaact cggcgctatg
     9961 ctggccagcc aaaggcaccg gggtcccgat gacgagggat gctacatcag tcccggcggc
    10021 ctcgccggcc tggcccacaa tcgtttgagc atccttgacc tttctcctgc gggccgacag
    10081 cccatgagca accgcgacgg atcgctgtgg attgcattta acggcgagat ctacaactat
    10141 ctcgagctgc gcacagagct gcgcggctat ccctaccgca gcaatacgga tacggaggtg
    10201 attctggcgg cctatgagcg ctggggaagg gcctgcctcg aacacttcat cgggatgttc
    10261 gcctttcttc tctgggatga gcgggaacaa acgctgtttg ccgcccgcga tcgattcggg
    10321 gtgaaacctc tctactacca ccgccaacgc ggcggcacgc tgctcgccgc cagcgaaatc
    10381 aagggcctgc atgccgccgg cgttcccgca gcgcccgacg ccggaacctg ggcgacatat
    10441 ctggctcgcg gcctgtatga ccattcccag aggacgttct gggacggcgt gcaatcggtt
    10501 cctcccggcc attttctcac ctggcggcgc gcggagacga aactcacgcg ctggtacgac
    10561 ttggccgagc gcgtaccgcc tgatttcgac agccgctccg ccgaggccgc cgccgaagaa
    10621 tatctggacc tcctgcaaga gagcgtgcag ctccggtttc gcgccgacgt ccccgtgggc
    10681 atcaatctca gcggcgggtt ggattcctcc acgctgctgg gcctgatcca gcgcctccag
    10741 ggggcggcga gcgaagtgcg ggcctatacg tttctcaccg gcgatccccg ctatgacgag
    10801 cttccctggg tcgagcagat gctccggcgc acgaggcatc cgttggttcc ctgccgactg
    10861 gagccaagcg atgttccccg cctggcgcgc tcggtccaga tgcaccagga cgagcccttt
    10921 ggcggcttgc ccactctcgc ctacgcgcgg ctgtttgaac gggcgcgcgc cgacggagtg
    10981 aaggtgctgc tcgatggcca gggcatggac gagcagtggg ccggatatga ttactaccaa
    11041 gacgttctga acgggaaaaa cggagccact atccaggggg cggccgattc gccggtccgg
    11101 cccgagtgcc tcgcgccgga atttcttgcg ctcgccgaaa actgggagat gccgcgcccc
    11161 ttcgccgata acctccgcaa ctgccaatat cgcgatgcgc gccacacgaa aatcccgcgc
    11221 gcgctccgct tcaacgaccg catatccatg cgggcatcga ccgagctgcg cgagcccttc
    11281 ttggatcacc ggcttttcga gctggcgctg cggcagccgg cggaccggaa gatcgccaat
    11341 ggcacacgca aggtgctgct ccgcgccatg gcgagagaac tgctgcccga gggagtggtg
    11401 gaagctccca agcggccctt gcaaacgccg cagcgcgagt ggctgagagg acctctgagg
    11461 ccgtgggccg aagagcagat ggaggcggca atttccgcct atggcggatc gtggctcaaa
    11521 ccggaaaagg tgagggctgc gtggcaggca ttcctgaatg gggagtccga taacagcttc
    11581 ttcgtgtggc aatggctgag tctggccatg attatggacg gggtatcggc atttacggca
    11641 tgagtgaact ggcaatccgg gtagcgggcc tttcgaaaaa gtaccgaatc ggcggccagc
    11701 gcgagcggta caagacgctg cgggattctc tcgccgacgc ggctgcggcg cccttccagc
    11761 gcctggcttc actaatggcc cggcggaaaa acgggaacgg ctgcggcgag agcgagatat
    11821 gggctttgtc caacgtggaa ttcgaggtga gccgagggga tgtcgtcggc gtgatcggcc
    11881 ataacggggc cggcaagagc acgctgctga aaattctctc tcgaatcacg gagccgacca
    11941 gcggccatgc ggagatacac ggccgggtgg ggtcgctgct ggaggttggc accggctttc
    12001 acctcgagtt gacgggacgg gaaaatatct acctgaatgg ggcgattctc ggcatgaagc
    12061 gggccgagat cgagcgaaaa ttcgacgaaa tcatcgcgtt cgccggggtg gagaagtttg
    12121 tggatactcc ggtgaagcat tactcgaccg gcatgcatct gcggctggcc ttcgccgtgg
    12181 ccgcccacct ggagccggag attctcctgg tggatgaagt cctcgccgtc ggagacgccg
    12241 catttcaaaa gaggtgcctg ggaaaaatcg gcgacatcgc cgcggagggc cgaaccgtcc
    12301 tgtttgtgag tcataacctg ggcgcggtga aggagctttg caacatgggt cttgtcctgc
    12361 gaaacggttt gttagccttc cgcggatctg ttgcgcaagc attggccttc tacggccgaa
    12421 cgctcaccga agcggctaat gacgagcact ccggacatgc tacagggtgg cgttacattt
    12481 atctgaatgg ctgctcggat ggctcgctcg cggtcaccac tcccgaagag ccgttgcaga
    12541 tccacgccgg gcttgaactg gatcagaatt gccagggcgg ccttgttttt ctcctgatcg
    12601 aagatttcat gggccagctt gttgtgcatc gccgggccgc tctctctacc atgtgttctg
    12661 agcccatagg gccgggccgt taccgggtaa ctagcaggct tcctgccgtg tggctggctc
    12721 ccggagttta caccgtctat ctgaaactta tcatcgagac tttcgacggc cagtatatgc
    12781 gctacttatc ggaaaagata tttctagagg ttgatggcgc taccgccggc ctgagccgtg
    12841 ccttactcgc tcctccgtgc gagtggtcgc tgacttcttg gcaggctccg ccgaccttcc
    12901 atcaggttgc tagtggcctg gaaacgcggt ccctcgcccg cccgctctga ctctccggcg
    12961 ccgattccag gccacacacg accgcaatct gcttggccgt tggtcaaggt tttatcccgt
    13021 gagtgcatac agcgatacgt tttatgccag gcaaggcgag ggctcgatca gctctgcccg
    13081 ccgtatcgtc cctctggtga ttgacctgat cgcgccgaaa tccgtggttg acgtcggctg
    13141 cggtctcggt tcgtggattg cggtgtttat ggaatccgga atacgggacg tcctaggcat
    13201 cgatggcccg tgggttagga aagagatgct tcatatctcg cctgagcatt tccaggaggc
    13261 cgatctagcg cgcccgctta ctctagaccg agggttcgat ctggtcgtgt cgctagaggt
    13321 ggcagaacat ctgccagctt cagcggctca gacgtttatt aattctctca cagctatggg
    13381 gccggcggtt ttattctcgg cggccatacc cttccaggga ggaatttacc acctgaatga
    13441 gcaatggcaa tcatattggg cggaaatgtt catgggaaga gaatatgtcc cagtcgactg
    13501 tatacggcct gtcatttgga acgatccaga cataaaggtc tggtacgccc aaaacacggt
    13561 cctgtatata cggcaaagcg ccctgccgtg ctatcccagg ctgaagtcct tttctattgc
    13621 atcctgcgac agcctttctc ttgtgcaccc aaggttgtac ctgggtaaaa tggaaaaact
    13681 tggccgcgaa ctccaaagca ctacccgcct ggggacgctt ttcgccgaga gcgccattcg
    13741 gcgactgccg ggagccgcgt tagctgcgcg ggtagttcga tcactcaggc attccatcaa
    13801 agactccgcc ccaagcgggc agtaggtcat gattacgcct tcggttaccg tcgtcatgcc
    13861 ggtgttcaac caagcgtgtt atgcccgcgc tgcgatcgac agcattctga atcagaccgt
    13921 ttcaaaattc gaatttctta tcgtcgacga cggttctacc gatggcacga gccatgtcct
    13981 gcacgcggtt acggatagcc gcatccgagt aatacgcatt ccccacagcg gatttctcac
    14041 cgcccttagg accggtgttg aagcagccag cggggatctt gtcgcccgga tggactctga
    14101 tgacctttgt cacccgaagc gcctcgagcg ccaaatcgct tttctcgagg cgcatcccga
    14161 atgcgtgttt gtcaccacgt attacggcct ggtgacgccc aggggatatt ttctcgcccc
    14221 caagcagagc ttcgactgga aatacgtcac tgcggctgac atcactcagg gcaacggcct
    14281 tttttgcgat cccggaacta tatttcatcg ccggcaggct gtatcggccg gactgtatga
    14341 ttccgaattt gaatatgaga cgccgctctg gtataagctt ctccgctacg ggcagggcgc
    14401 cgtcctagga gcgtcactct actacgcccg gtggctcgtg agctcctatt ccagagatga
    14461 gagcggattg cggcgccgaa gacaagttca ccgtttgata cgactcaagt acgacccgga
    14521 gggcgcaaca aagattcctg ccaaggctag ccccaaaaac gagccgtcgg gcatcagcgt
    14581 ggcccggcgc gccatcggac tttaccttct ggcaggagat aaggcgtctg cccgggaggt
    14641 cgccataaaa gcatgga
//
LOCUS       DASREK010000103         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_57425, whole genome shotgun sequence.
ACCESSION   DASREK010000103 DASREK010000000
VERSION     DASREK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57425"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFA54_05210"
     CDS             complement(<1..581)
                     /locus_tag="VFA54_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50237.1"
                     /translation="MRRRDFLLQSSAALLAARGKAASWDAGSVAHLLPTVNDRRILLK
                     ASFQAPLSRAPSLRAGKSFFPGTRMDTAGLFWAFDATGLGPGRSHELQLISDSRRPLC
                     DPWTLKTFPDPQDQPARFRLLIYTCAGGHDALTGRDGQPRFLSLARRRRLLERGLSFQ
                     PDAVIANGDHIYWDLRTKNAKFLGESPRGIAFAG"
     gene            complement(693..1718)
                     /locus_tag="VFA54_05215"
     CDS             complement(693..1718)
                     /locus_tag="VFA54_05215"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZS50238.1"
                     /translation="MSFFTKRAALTCEGAIAVLSACWLASALALSRPAQAQSHPAITK
                     ETFDQWMTELSNWGRWGKDDELGAINLIMPAKRKQAAALVKEGVSFSLSRSAEKEKAA
                     DNPAPFGHQMIRTGINNPGTSVADTFFISHHGYAHTHMDSLCHFFYKGKMYNGFPQEM
                     VTERGAAKNAIFNFKNGIFTRGVLMDIPRLKGVEYLEPGTPIYPEDLDAWEKKARVRV
                     GAGDVVFIRTGRWALRDAKGPWEISHKAAGLYASCARWLKSRDAAMVGSDAASDVLPS
                     GVEGVTQPIHTLVLVAMGMPIFDNCDLELISKEANRRQRWDFLITAAPLAVPGATGSA
                     LNPIATF"
     gene            complement(1812..>2441)
                     /locus_tag="VFA54_05220"
     CDS             complement(1812..>2441)
                     /locus_tag="VFA54_05220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZS50239.1"
                     /translation="TTLWYQIYPEADMAPVLTRVRQAVKLGCKVVCLTVGTPYQPAGA
                     NGPPNPSKLAVEGNPHMDWAIVDRVRQAAQAPVVLKGIMSADEARVAVEKGVQGIIVS
                     NHGGRFVQGLASPIEVLAPVVDAIGAKAPVMIDGGLRRGSDIVKALALGARAVLVVRP
                     PLWGLAAYGSDGVQTVMQMLISETARDMGLCGKPTLADLNRTLVRMVRR"
BASE COUNT          489 a          783 c          717 g          452 t
ORIGIN      
        1 cctgcaaatg ctatgccgcg aggcgactcg ccgaggaatt tggcattctt ggtgcgcaga
       61 tcccaataga tgtggtcgcc gtttgcaatc accgcgtccg gctggaagga gagtccccgc
      121 tccagtagcc ggcggcggcg ggcgagagac aaaaagcggg gctggccgtc gcggccggtc
      181 agagcatcat gcccgcctgc acatgtataa atcagaaggc gaaacctggc cggctggtcc
      241 tgaggatccg ggaaggtttt taaggtccac ggatcgcaca ggggccgccg cgagtcgctg
      301 attaactgca actcgtggct gcggccaggg cccagccctg ttgcgtcgaa cgcccagaac
      361 aggccggcgg tgtccatgcg cgttccaggg aagaaactct tgccggcgcg caggctaggg
      421 gcccggctca gaggagcttg aaaggaggct ttgaggagaa tgcgccgatc attgaccgtc
      481 gggagcaagt gggctacgga tccggcatcc cacgaagcgg ctttccccct ggccgccagc
      541 agcgccgcgg agctttgtaa cagaaaatct cttcgccgca cggtggcact ataccaatac
      601 cgtcgttcgt ccgacaacag ccggcgctgg ccggcgcggc aaacaggcgt ggccgcttcg
      661 ttgacgcggc acaaacacga ctgtcacacg cctcaaaacg ttgcgatcgg attcagcgcg
      721 gaaccggtag cccctggtac ggccagcggc gccgcggtga tgagaaaatc ccagcgctgc
      781 cgccggtttg cctctttgct gatcaattcc aggtcgcaat tgtcgaaaat gggcattccc
      841 atggcgacca acaccagcgt gtgaatcggc tgggtaacgc cttcgacgcc ggaaggcagc
      901 acgtcgctgg ccgcatcgct gcccaccatc gccgcatccc gcgatttcag ccaccgggcg
      961 caggaagcat acaggcccgc cgccttgtgc gagatctccc aagggccttt cgcgtcccgc
     1021 aacgcccagc gtcccgtccg gatgaaaact acatcccccg ctcctaccct gacgcgggct
     1081 ttcttctccc aggcatccag gtcttccgga tagatcgggg tccccggctc caggtattcc
     1141 acgcccttca ggcgcggaat atccattaag actccgcgcg tgaaaattcc gtttttgaag
     1201 ttgaatatcg cgtttttcgc cgcgccccgc tcggtcacca tctcctgggg aaatccatta
     1261 tacattttgc ccttgtagaa gaaatggcaa agcgaatcca tgtgagtgtg ggcatagccg
     1321 tgatgcgaga tgaaaaacgt atcggcgacc gaagtccccg gattgttgat accggtcctg
     1381 atcatctggt gtccaaaagg agcaggatta tcggctgcct tttccttctc agcgcttcgc
     1441 gacagcgaga aagacacgcc ctccttcacc aaagcggccg cttgttttcg cttggcgggc
     1501 ataatcaggt tgattgcccc cagctcgtcg tccttacccc agcggcccca gttggagagc
     1561 tcggtcatcc attgatcaaa cgtttccttg gtgattgccg ggtgggattg cgcctgagcc
     1621 ggacggctca gcgccagcgc tgaggccagc cagcaggcag acagcacggc gatagcaccc
     1681 tcgcacgtga gagcggctct tttcgtaaaa aatgacatgt gttcctcttc gatgagtggc
     1741 cgacggccga tcggccgcaa aacaatgcag caatgcagcc ttgacggccg catgactgac
     1801 ccagcggcct gtcagcgccg caccatgcgc accagagtgc gattcagatc cgccagagtg
     1861 ggtttgccgc agagccccat gtcgcgcgcc gtctcgctaa tcagcatttg catgacggtc
     1921 tgcacgccgt cgcttccgta agccgccaga ccccagagcg gcgggcgcac aaccaaaacc
     1981 gcgcgagcgc cgagcgcaag agctttcacg atatcgctgc cgcggcggag gccgccatcg
     2041 atcattaccg gcgccttggc cccgatggca tccactacgg gcgccagcac ctcgatgggc
     2101 gaagcaagac cctgcacgaa gcggccgccg tgattggaaa cgatgatgcc ctggacccct
     2161 ttctccacgg caacccgggc ttcgtcggcg ctcatgatgc ctttcaatac gacgggcgcc
     2221 tgagccgcct gacgcacacg gtccactatc gcccaatcca tatgcgggtt tccctccaca
     2281 gcaagctttg atggattggg agggccgtta gccccggccg gctgatacgg agtgcccaca
     2341 gtcaggcaca caactttgca cccgagcttc actgcctgac gcacgcgggt caataccgga
     2401 gccatgtcgg cttcagggta gatctgatac caaagcgtcg t
//
LOCUS       DASREK010000104        14295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2037007, whole genome shotgun sequence.
ACCESSION   DASREK010000104 DASREK010000000
VERSION     DASREK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 14295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14295
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037007"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VFA54_05225"
     CDS             <1..1384
                     /locus_tag="VFA54_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZS50240.1"
                     /translation="YDQIGGGFHRYSTDSRWLAPHFEKMLYDNALLISAYLEAYQATG
                     RRDFAQIARDTLRYVERDMLSPEGAFYSATDADSAGPDGAPEEGRFFTWTPEEMESAL
                     GPDRFRLAKAYFGVTTQGNFHGRNILHVDRPLDLVAGELGIPGGKAHSMLEESKRLLY
                     DARGKRPRPLRDEKILTSWNGLMISACAQAALALGDDRYARLGAGAADFILRNLRREG
                     RLIRTYSNGRAGRTAYLDDYAFFIGGLLDLYEATGELRWLSAALDLDAALARHYEDPA
                     GGFFLTSDDDEKLLVRHKPSFDGPEPSGNSVAVRNLLRLAELTSSERFRERAERALTT
                     FNGILSEAPAYLSDMLLGVDFALDTPKEIVIVTPHSRSEAEPLLEQLRRRFVPNRILV
                     VAPQGTALAAAQKLIPLVENKIAQDGRATAYVCEKRVCKLPTADPEVFAGQITARPPA
                     LDPAGGAMRR"
     gene            1528..1788
                     /locus_tag="VFA54_05230"
     CDS             1528..1788
                     /locus_tag="VFA54_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HZS50241.1"
                     /translation="MAYVIAEPCIGTKDTACVDACPVDCIHPKKDEPNHAEAKMLYID
                     PVECIDCGACVPVCPVSAIFALDDLPEKWQHFAAENAAYYGR"
     gene            2117..3163
                     /locus_tag="VFA54_05235"
     CDS             2117..3163
                     /locus_tag="VFA54_05235"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS50242.1"
                     /translation="MASDYSKLLRGMIRYYPVVLKNFRTYLGNLYIRRIKKSNVVRPY
                     SAVFYATHKCNLDCSYCTQKNPEVFSEELDTARTIEVLHAIRRGVDTILFTGGEPLIR
                     PDIGELVRAARREAKFLDVLLVTNGVLLDQRKEILDHLTGLVVSLDAITPEPNGRLTK
                     SSVVQRVLDNLDMVRSKLPPRAVTISCVLEEWNIGEAERILEYCAGHGHVFSVQSAQN
                     GLHPDFSLLYNRRYRDFVDKLVRLRSERKIPINGTPKLLRTLLEFKDFHCYPTMFPRV
                     YPNGDVFYPCEPLRKIAGNVLEDGGFAKIFERGRRLYGEVPECKSICYLFGNVVSSFY
                     VEDFWGFAGDTIRR"
     gene            complement(3216..3371)
                     /locus_tag="VFA54_05240"
     CDS             complement(3216..3371)
                     /locus_tag="VFA54_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50243.1"
                     /translation="MDSVLRALKEVIAAHPAGPIRRALRPGGLCRGWNQKEMHKGDRC
                     SVMMSWP"
     gene            complement(3399..4124)
                     /locus_tag="VFA54_05245"
     CDS             complement(3399..4124)
                     /locus_tag="VFA54_05245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50244.1"
                     /translation="MTDEVWKDETLVRAFLAGVRGGLPFAAAQIEILLRIVYALGRPI
                     ESLADLGCGDGILARAILAKFPAASATLVDFSEPMLEQARGQLSSEGRDVCFVPADFG
                     EKQWSGLIAGRGPFDLILSGYAIHHQPHQRKRELYQEIFELLKPGGLFLNTEHVSSPT
                     NWVRSISDGLYIDSICKFQRSQGSQKGRDEIAREFFHRPDKAANILASVESQCDWLRA
                     AGFVDVDCYFKVFEAAIFGGRRP"
     gene            complement(4121..4855)
                     /locus_tag="VFA54_05250"
     CDS             complement(4121..4855)
                     /locus_tag="VFA54_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50245.1"
                     /translation="MSEVRIRSNETGRGAKRGFTSRLAAAALAPLFALWLLVPLLRRE
                     RRRRGWKWVRICGLMAGAALLAAGWKLRPLLFAGATLLLLSALLGPLADPGQLHALAE
                     RLGAKHILNGGFFAGGEACLPVGAAVLLFLTAEELLAAAAESPGRKQWRWPLAEVKNI
                     QIDGQDYHPRDVGFAKAPPARDQNVSRNAACLLALHLAGENEHQLYFEYKGVFARHLA
                     EVAAHTIYRCRALAAKAPVQILRVIR"
     gene            5408..5920
                     /locus_tag="VFA54_05255"
     CDS             5408..5920
                     /locus_tag="VFA54_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS50246.1"
                     /translation="MEFDPRTLEGYRLKLRYKACYHLGSFCPDVDDIVQETLARFVTA
                     LRDKKVHNPESIGAFLSGICNNVIHEYWRRLWREAPPDSALPEHPAVPPASEAVELRQ
                     AVAAALAQLSDRDCELLRAFFLEEQDKDEICRRTGLSDSQFRVALFRAKERFRKIYRQ
                     EMKHKSPGVH"
     gene            5938..6444
                     /locus_tag="VFA54_05260"
     CDS             5938..6444
                     /locus_tag="VFA54_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZS50247.1"
                     /translation="MEYHEDPLLREELIRNYLLKRLDESATKAFESHYLICDECFEEL
                     RASELLMGGLSWSKVDRRRLEDVVVIAFSGPVQLTRQSEMTGLLRGMLEQKDTKVLID
                     LSRVSRIDSAGLGMLINCYSHAVRNSGMLKLLNPSMEVQRLLHITHIDSVLETYYDER
                     QAVASFNS"
     gene            6644..10138
                     /locus_tag="VFA54_05265"
     CDS             6644..10138
                     /locus_tag="VFA54_05265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50248.1"
                     /translation="MQRRIWSLLVWILFPCLAWSQSYTASVRGTVTDAAQAAVPSASV
                     VVTEVNRNLPHSTKTDVAGRYILPALPPGTYSLSVEAPGFQKYMRPATFQLEVQQEAT
                     INVQLSLATVSTSIEVEGTAPLLNTASATLGQVVENRFMATAPLVNRTALSLVLLTPG
                     VVPSDNSPGGTANTNFIANGNRNRTSQAMLDGTNVSGIEQNGGWTDIEYSPSVDAIQE
                     FKVQTNFFNAEFGETGGAIVNIITKSGTNDPHGSLYEFTRNSGLNANNWFSNRAGLPI
                     PDFQRHVFGGTLGGPVSLPKIYNGKNRTFFFFDYEGARQSTATSVTTTVPTDLQRAGD
                     FSQTLNSNGKLSVIYNPFDTYVSNGQTLRNPFPGNVVPPSMMNAVAKKLISYYPEPTS
                     AGNPVTHSNNYFAQGANELTSNQMDAKIDQTVSDKQRIMGRYSVYWQHTTPANLLGNI
                     ADSGNFVPSRNQNFVLNYTRTHSPTTIFEARIGELRVHDDQLPRSTGFDSTTLGFPKV
                     LQTGGVYQFPNVSASGYRTLGAGGFSLIHEGKETRTASGAFTKIIGGHTIKGGAERLF
                     LYENYFQPGYPAGGFSFSRQVTGQNPLVGTSSQGNAIASLLLGWGQGGYMWWDHPVAA
                     ASGYFGSFIQDDWRITRKLTLNIGLRYEFDVPRTERFDRLDYFDYYSPSPLAGKVPAS
                     PNCPACSNLLGVMKFVNSSRRSPFDGDYNNFGPRIGLAYALGNKMSVRAAYGIFYSQN
                     RDSIKGEVGPTFRSSTSVLWTLDGGLTQYASLSNPYPSGLTPAPGRNPLAWIGHGVDS
                     YIPSYINPQIQQWLFSIQRELPGNAVLEVNYSASKATHNYFGGEDLPGNYNKLNPVYW
                     GLGRTALESLVPNPFHGIITDPTSPESLPTVPLNVLLRPYPQYDGGLGEYNAPPNIAN
                     SMYHSMQLKYEKRFSKGLAVLASYTISKLISDSDESSSDVDWQGGSSGVQNAWNLRLE
                     RSLSQFDIPQRAVISLDYELPLGRGRRFGSSMNGVAEALVGGWEVATILTFSSGYPIV
                     PGLDSPNLWDGASQRPNIIGNPCTSGSVESRMNGSYFNLSAFSQPDEDVFGTAPRTLP
                     ACRTPGIRNADIVLMKNFRFKERQQLQLRLESFNVTNTPTFGRPDSNYGSNTFGVISG
                     YASGRGPREMQVAAKFYF"
     gene            10371..11516
                     /locus_tag="VFA54_05270"
     CDS             10371..11516
                     /locus_tag="VFA54_05270"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50249.1"
                     /translation="MPKFHGRHLLGVAALLVFFASVSRAQVSTLQGEVKDENGKPLQN
                     AMVHIERKDIKGNYQVKTNKKGQYLHAGLPLGVYKVSVEVNGKTMDTVDNVRTKLGDP
                     TTVDFNLAEAKARAEAAAAGMELNENRQMTAEQRRQIEEATKRRQEQLSKNKALNDAF
                     NAGMEAMRAKNWESAIDSLTKASEIDPKQHVVWANLGEATMNLASAKPPAEQPPLIAK
                     AMEDYTKAIEIKPDDAAYHNNYGLALARAGKYPEAQAELSKAAELDKPNAGRYFFNLG
                     AILVNTGHNDEAYEAFKKAVAADPNYADAYYQIGVYLLSKAQVAADGKVTPAPGTAEA
                     FQKYLELAPNGPNAASAKGSLQAISGSVQTQYGTEKPAPAPAAKKKR"
     gene            11517..11804
                     /locus_tag="VFA54_05275"
     CDS             11517..11804
                     /locus_tag="VFA54_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50250.1"
                     /translation="MRFLLGLIAILAAFILLRSLLEPVVKAVVALLTPPAATHTAAGV
                     RSGSAPRASELKKDPVCGTFVAPELAVTQEIRGQIMHFCSPKCRDEYSRRA"
     gene            11935..13494
                     /locus_tag="VFA54_05280"
     CDS             11935..13494
                     /locus_tag="VFA54_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZS50251.1"
                     /translation="MSDISRRRFNETLVSGIGASLVTASPLHPQASSAKKPNILFICA
                     DQHSGLVMGPNGHPIVRTPNLDRLAARGVNFRNAYCGSPVCVPSRAGLMTGMFPSDVG
                     SYCNSTHFDGRVPTWGNRLRDAGYHCWATGKLDLAPGKDYGFREVHTANGHAEHPDIT
                     SLFRAPLCFRVDERRQIDGRFQQRVHSDDAHVKYALDFLRNEAPRQQNPWAAYVGLTL
                     PHPSFVALPKYAETYRPEDMPLPNIPAGYLEQRHIVFQALANFKLISTPIPEARVRRA
                     RAAYFGMITELDAYIGNLLDELEGSGLLDNTLVVYTSDHGEMLGEHGLWLKNNLLEGS
                     ARVPLIMAGPGLPRGRTVETPVAHVDLVATMLDLAAASTAGGLRGRSLLRLAHGQPGN
                     HPGYAVSESHSEGNCTGSFMVRKDNWKYIYFSWYEPLLFNLKEDPGELRNLAGKPETT
                     SVQKELHGILTSLLDPDAVTGRAFAEQERRFLQMARERPADSFYKELAGRLGPGQARA
                     ITNKYYKNRNG"
     gene            complement(13614..>14295)
                     /gene="kgd"
                     /locus_tag="VFA54_05285"
     CDS             complement(13614..>14295)
                     /gene="kgd"
                     /locus_tag="VFA54_05285"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF028180.4"
                     /note="kgd; produces succinic semialdehyde; part of
                     alternative pathway from alpha-ketoglutarate to succinate;
                     essential for normal growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HZS50252.1"
                     /translation="LLPHGYEGQGPEHSSARIERFLQLAGEDNLQIAQPSTAAQYFHL
                     LRRQAVRRWRKPLVAFTPKSMLRDPRACSPIEAFTQGRFLTVIADSESQQPDRVLLCT
                     GKIGHELRSERQRRGDSSTAILFLEQLYPFPEQELARELDRYPNAEIVWVQEEPANMG
                     ALFFVQPRLRRLAGKRPFRSVKRSASASPATGSAKAHAIEQAALLALAFGPGRSATRN
                     ESLARKVS"
BASE COUNT         2897 a         4319 c         4277 g         2802 t
ORIGIN      
        1 ttacgatcag atcggcggcg ggtttcatcg gtattcgaca gattcccgct ggctggcgcc
       61 gcacttcgaa aaaatgctgt acgataacgc cctgctgatt tccgcctacc tggaagccta
      121 tcaggccacg ggacgccggg acttcgccca gatcgcccgc gacacgctgc gatatgtcga
      181 gcgcgacatg ctttctccgg aaggcgcgtt ctactccgcg accgacgccg atagcgccgg
      241 tcccgacggg gcgccggaag aaggccggtt tttcacctgg acgccggagg agatggaatc
      301 ggctctcggg ccagaccgat tccgcctggc gaaagcttac ttcggcgtta caacacaggg
      361 caatttccat ggccggaaca ttctccatgt ggaccgcccg ttggatctcg tggccggcga
      421 gctcggcatt ccgggcggca aagcgcattc gatgctcgag gaatcgaagc gattgctcta
      481 cgatgcgcgc ggcaaacgtc cccgcccgct gcgggatgag aaaatcctga cttcgtggaa
      541 cggcctgatg atttccgcct gcgcacaggc tgcgctggcg ctgggcgacg atcgctacgc
      601 ccggctcggg gcgggtgcgg ccgatttcat tctgcgaaat ttgcggcggg agggccgcct
      661 gatacggacc tacagcaacg gcagggccgg gcgcaccgcc tacctggacg attatgcgtt
      721 cttcatcggc gggctgctcg atctctacga ggccaccggc gaattgcggt ggctgagcgc
      781 cgctctcgat ctcgacgccg ctttggcgcg gcattatgaa gatccagccg gaggcttctt
      841 tctgaccagc gacgatgacg aaaagcttct ggtgcgccac aagccgagct tcgacggtcc
      901 ggagccctcg ggcaactccg tggcggtccg caacctgttg cggctggccg aactgacctc
      961 cagcgaacgc ttccgcgagc gcgccgagcg cgctttgacc acgttcaacg gcattctgag
     1021 cgaagcgccg gcgtaccttt ccgacatgct gctcggtgtg gatttcgccc tggacacccc
     1081 gaaagaaatc gtaatcgtta ccccgcactc gcgcagcgag gccgagcctc tgctggagca
     1141 actgcggcgg agattcgtac ccaatagaat cctggtggtg gcgccgcaag gcaccgccct
     1201 ggccgccgcg cagaaactga tcccgctggt ggaaaacaag atcgcccagg atgggcgggc
     1261 cacggcttac gtctgcgaga agcgggtatg caagctgccc accgccgacc ctgaggtctt
     1321 tgcgggccag attacagcca gaccgccggc gcttgatccg gccggcggcg ccatgcgccg
     1381 atgatcgtgg gccgggccgc tgtgcggctg aaaacagccg gatccccgcc atcgccttcc
     1441 gtccgggcca ctttcttttt ctcactgccc atgctatatg tgtgagttaa agctgggccg
     1501 cttgccctaa cattggagaa ctgaaacatg gcttacgtca tcgccgaacc ttgcatcggc
     1561 acgaaagaca ctgcgtgcgt ggacgcatgc ccggtcgatt gcattcatcc taaaaaggac
     1621 gagcccaatc acgccgaagc caagatgctc tacatcgatc cggtggagtg catcgattgc
     1681 ggcgcgtgcg ttccggtttg tccggtttcg gctatctttg ctctcgatga tctgccggaa
     1741 aagtggcagc atttcgcggc ggaaaacgcc gcctactacg ggcgatagcc tcggggtctt
     1801 ttgagcgctg catctcgcag caccagccac aagtctgaac acgttacaaa ataggatttg
     1861 cggcgcacgc aggaggcgct gtgcgccgcc gttccgctca agctgccgcg cctttctgaa
     1921 ctgggcagac ggcgccggga gcgccgcgct gcgctgccgc atgcgctcct gaagctcaca
     1981 gcgccggggg ctcttctctg atcggaaatt ggcgtctcct gcgtcgctgt ttgcaggcag
     2041 cgtgcggccg gaacaaccag cgttgcgttg agccagacac agaggccgtc cgttacgcgg
     2101 cggtacactg gatgccatgg cgtccgacta ctccaagctg ctgcggggca tgatccgcta
     2161 ctatccggtt gttctaaaaa acttccggac ctacttgggt aatctttata tccgccggat
     2221 caaaaagagc aatgtggtgc gtccctactc ggcggtgttc tatgccacgc acaagtgcaa
     2281 cctggactgc tcctattgca ctcaaaagaa ccccgaggtt tttagcgaag agctggatac
     2341 ggcgaggacc attgaagtgc tacacgccat tcgccgcggc gtggatacga ttctcttcac
     2401 gggcggggag ccgctgatcc ggcccgacat tggcgagctg gtgcgcgccg cccggcgcga
     2461 agcgaagttt ctcgacgttt tgctggtgac caacggcgtg ctgctcgacc agcggaagga
     2521 aatcctcgat catcttaccg gcctcgtcgt gagcctggac gccatcaccc cggagccgaa
     2581 cggccgcctg accaagtcga gcgtggtcca gcgggtgctc gacaacctcg atatggtgcg
     2641 ctcgaagctt ccgccgcgcg cggtgactat cagttgcgtt ctcgaggagt ggaacatcgg
     2701 cgaggcggag cggattctgg agtactgcgc cgggcacggc catgtatttt cagtacaatc
     2761 cgcccagaac ggcctgcacc ccgacttctc gctgctctat aaccgccgct accgggattt
     2821 tgtggataag ctggtgcggc tgcgctccga acgaaagatc cccatcaacg gcaccccgaa
     2881 gcttctgcgg actctgctcg agttcaagga ttttcattgt tatccaacga tgttcccgcg
     2941 tgtttatccc aacggcgacg tgttttaccc ttgcgagccg ctgcgcaaga tcgccggcaa
     3001 tgtgctggaa gacggcggct tcgccaaaat tttcgagcgg ggccgccgtc tttacggcga
     3061 agtcccggaa tgcaagtcga tctgctactt gttcggcaat gtcgtctcgt cattctacgt
     3121 agaagatttc tggggcttcg ccggcgatac catccgccga tgagccgaag gacggctgcc
     3181 gcggccaagc cgctttccgc aacgagcagc aggccttagg gccaggacat catcaccgag
     3241 caccggtccc ctttgtgcat ttccttctga ttccatcccc ggcacaagcc gcccggacga
     3301 agcgcgcgcc ggattgggcc ggcgggatgt gcggcgatga cctctttgag agctcggaga
     3361 acagaatcca ttacaggctt cttccccggg gatcagcgct acggacggcg cccgccgaaa
     3421 atcgccgcct cgaatacttt gaaataacaa tcgacgtcaa cgaatcccgc ggcgcgcagc
     3481 cagtcgcatt gcgactcaac cgacgcaagg atattagccg ctttatccgg gcgatgaaaa
     3541 aactcacggg caatctcgtc gcggcctttc tgggagcctt gactgcgttg aaacttacaa
     3601 atggaatcga tgtagagacc atcggaaatg gaccgcaccc agttggtcgg cgatgagacg
     3661 tgctctgtgt tcagaaacag tccgcccggc ttgagcaact cgaaaatttc ctgatagagc
     3721 tcccgctttc gctggtgcgg ctggtgatga atggcgtagc ctgacaggat caggtcgaag
     3781 gggccgcgcc cggctatcag accgctccac tgcttctcgc cgaaatccgc cggcacaaaa
     3841 cagacgtcgc gtccttcgct ggaaagctgg ccccgggctt gctccagcat aggctcagaa
     3901 aagtccacca gggtggccga cgcggcgggg aacttggcta gaatggcacg cgcgaggatg
     3961 ccgtcgccgc agcccaggtc ggccagcgat tcgatgggcc tgccgagagc ataaacaatc
     4021 cgcagcagaa tctcgatctg cgcggccgcg aagggcagcc cgccccgcac tccggccaga
     4081 aaggcgcgaa ccagcgtttc gtctttccag acctcgtctg tcatcggatc acccgcagga
     4141 tttggacggg cgcctttgcc gccagggccc ggcagcggta aatggtatgc gccgcaactt
     4201 cggccaggtg gcgcgcaaaa acgcctttgt attcgaagta cagctgatgc tcgttttctc
     4261 cggcgaggtg gagcgccaga aggcaggcag cgttgcggct aacgttttgg tctcgcgccg
     4321 gcggagcctt ggcgaaaccg acgtcgcggg ggtggtagtc ctggccatca atttggatat
     4381 ttttcacttc ggccagcggc caccgccatt gtttccgccc gggagactca gcggctgccg
     4441 ccagcagctc ttctgcggtg agaaacagca ataccgcagc gcccaccggc aggcatgctt
     4501 cgccgccggc gaagaacccc ccattcagaa tatgctttgc tccgagcctt tcggccagag
     4561 cgtgtagttg gccgggatcg gccagcggcc ccaacagcgc cgagagcagc agaagcgtgg
     4621 cgcccgcaaa caacaggggg cgcagtttcc agccggcggc gagcagcgcc gcgcccgcca
     4681 tcagtccgca aatccggacc cacttccacc cccggcgccg ccgctcgcgc cgcaaaaggg
     4741 gtacgagcag ccagagggcg aacaatgggg ccaaagccgc tgcggccaaa cgagaggtaa
     4801 acccgcgctt ggcgccacgc ccggtctcgt tgctgcgaat gcgcacttcg ctcatgagat
     4861 cacgtcctat ccaccatatc gcaatatccg gcgcgcaagc agcgcggctt tcttttcggc
     4921 cctgtataat tcgacgtgaa tctggcggct gataggggag aggagtgcga actttggttc
     4981 ggagaaagac cgcgggtttg gtggctgtga aaaaaatgcc ggcgtaccac gcccgtaaaa
     5041 acgagtggct cccatacttg cagttcactc gtttttatgt cttaatgcaa cactgtgaaa
     5101 acgatgcaga gtcccggctg ctgcgaatcg gccgagccct tctatcgccg gaaaccagcc
     5161 gccggggcct ccggcggtat gcggagattt cggagtggga atggaaagga atcacggcgg
     5221 agaagcgcgg caggactcac gccggttcgc gaggagtaat tggcagagaa catgcacatg
     5281 tgacggcgga ggtccagctg gccagattta cgcctatttg ttttgacgcc ggcccttctc
     5341 cggggccggc ggccccggct atcacgggcc gctggcgctt tccgcatttt ttccgggtgc
     5401 ttccgaaatg gaattcgatc cccgaacgct cgaaggctac cggctcaagc tgcggtataa
     5461 ggcctgctat catttaggat cgttttgtcc agatgtcgac gacattgtac aggagacgct
     5521 tgcccggttt gtcaccgcct tgcgggacaa gaaggtgcac aacccggaga gcatcggagc
     5581 cttcctcagc gggatttgca acaacgtgat tcacgaatac tggaggcgcc tgtggcgcga
     5641 ggcccctccg gattcggctt tgcccgagca ccctgccgtt ccaccagctt ccgaggccgt
     5701 ggagctgcgg caggcggtgg ccgccgccct ggcgcagctg tccgacagag actgtgagct
     5761 tttgcgggct tttttcctgg aggagcagga taaagacgaa atttgccgca ggaccggcct
     5821 ttcggacagc caattccgcg tggcgttgtt ccgagccaag gagcgttttc ggaaaattta
     5881 tcgccaggag atgaaacaca agtcccccgg ggttcactaa ttatcaggag agcaggcatg
     5941 gagtaccacg aggatccatt gctccgagaa gagctgatcc gaaactacct gctgaaacgg
     6001 ctggatgaat cggcgacaaa agcattcgag agccattatt tgatctgtga cgaatgcttc
     6061 gaggagctga gggcctccga attgctgatg ggcggccttt cttggtccaa ggtggacagg
     6121 cgccggctgg aagatgttgt ggtgatcgcc ttttccggcc ccgtgcaatt aacccggcag
     6181 tccgagatga cggggctgct gcgcggcatg ctggagcaga aggacaccaa ggtgctgatc
     6241 gatttgagcc gggtaagccg gattgacagc gccgggctgg gaatgcttat caactgttac
     6301 tcccatgccg tccgcaacag cgggatgctc aaacttctga acccgagcat ggaggtgcag
     6361 cggctgctgc acatcaccca catcgactcc gtgctcgaga cctactacga cgagcgccag
     6421 gcggtagcca gcttcaattc ttagaagcgc ggccagcttc cggtttcgaa cttcccccaa
     6481 gcatcccggc ggccgtagcg tgctcgtgcg tgagcgcggc ggaaaaccga aattcctgga
     6541 cgctgaagag actccgaaaa ttaacgctaa agcctggaga tttctatatt gacatcgccg
     6601 ccgggcgggt gtagcatacg gccaattttt tggggaggaa caaatgcaaa ggcgaatttg
     6661 gtctctcctt gtttggattc tgtttccttg tcttgcctgg tcacagtctt acaccgcgtc
     6721 cgtgcgcgga actgttacgg acgcagcgca ggccgccgtt ccctccgctt cggtggtggt
     6781 gaccgaggtg aaccgcaatt tgccgcacag cacgaaaacc gatgtagcgg gccggtacat
     6841 ccttccggcg cttccgcccg gcacctatag ccttagtgta gaggcgcccg gctttcaaaa
     6901 atacatgcgg cctgcgactt tccaactgga ggtgcagcag gaggccacga ttaacgtcca
     6961 gctcagcctg gcgactgttt ccacctcgat tgaagtggaa ggcacggcgc cgctgttgaa
     7021 cactgccagc gcgacgctgg ggcaggtcgt ggagaaccgg ttcatggcga ccgcccctct
     7081 ggtaaaccgc acggctttga gcttagtgct tctgacgccg ggcgtggtcc ccagtgacaa
     7141 cagtcccgga gggacggcaa acaccaactt cattgccaac ggaaaccgga accggacctc
     7201 gcaagcgatg ctggatggca cgaatgtttc gggcatcgag cagaacggcg gctggaccga
     7261 catcgagtac tcgccttcag tggatgccat ccaggagttc aaagtgcaaa cgaacttctt
     7321 caacgccgag ttcggcgaga caggcggcgc catcgtcaac atcatcacca agagcggtac
     7381 caacgacccg cacggctcgc tctacgagtt cacccgcaac tccgggctga acgcgaacaa
     7441 ctggttctcg aaccgcgccg gcctgcccat cccggatttc cagcgtcatg ttttcggcgg
     7501 caccttgggc ggccccgtgt ctctccccaa gatctacaac ggcaaaaacc gcaccttctt
     7561 tttctttgac tatgagggag cccgccagag caccgccact tcggtaacga ccacggtgcc
     7621 cacggatttg cagcgcgcgg gagacttttc gcaaaccttg aattccaacg gcaagctgtc
     7681 ggtgatttac aacccgttcg acacctacgt ctccaacgga cagaccctgc ggaatccctt
     7741 tcccggaaac gtagttcccc cgtccatgat gaacgcggtg gcgaagaagc tgatctctta
     7801 ctatccggag cccacttcgg ccggcaaccc ggtgacccac tccaacaatt actttgcgca
     7861 gggcgcgaac gagctgactt cgaatcagat ggatgcgaaa atcgatcaga cggtcagcga
     7921 caaacagcgc attatggggc gctacagcgt ttactggcag cacaccacgc cggccaacct
     7981 gttgggaaat atcgccgaca gcggcaactt cgttcccagc cgcaaccaga atttcgtttt
     8041 gaactatact cgtacgcaca gccctactac gattttcgag gcgcggatcg gcgaactccg
     8101 ggtccacgac gatcaattgc cgcgcagcac gggcttcgat tcgacgacct tgggtttccc
     8161 caaggtcctt cagacggggg gagtctatca gttcccgaat gtttctgctt ccggctatcg
     8221 aactctgggc gcggggggat tttccttgat ccatgaaggc aaggaaacgc gcacagccag
     8281 cggagctttt accaaaatta tcggcgggca cacgattaaa ggcggagccg agcgcctgtt
     8341 tctgtatgag aactactttc agcccgggta tccggcgggc ggattcagct tcagcaggca
     8401 ggtcacgggc cagaatccgc tggtcggcac ttcaagccag ggcaatgcca tcgcctcgct
     8461 gctgctcggc tgggggcaag gcgggtacat gtggtgggac catcccgtgg cagcggcctc
     8521 cggctatttc ggcagcttca tacaggacga ctggcgaatc acccggaagc taaccctcaa
     8581 tatcggcttg cgctatgaat tcgacgttcc ccgcacggag cgtttcgacc gtcttgacta
     8641 tttcgactac tattcaccgt ctccgctggc gggcaaggtt ccggccagcc ccaattgccc
     8701 ggcgtgcagc aaccttttgg gcgtcatgaa attcgtgaat tccagccgcc gcagcccgtt
     8761 tgacggggac tacaacaact tcgggccgcg catcgggctg gcttacgcgc tgggaaacaa
     8821 aatgtcggtt cgagccgcgt acggcatctt ctattcccag aaccgcgact cgatcaaggg
     8881 tgaggtcgga cccacgttcc gctccagcac gagtgttctg tggacgctcg acggaggctt
     8941 gacgcagtat gcgtccctct cgaaccctta tccgagcggg ctgacgccgg cgccgggaag
     9001 aaatcccctg gcgtggattg gtcatggcgt cgattcctat atacctagct acatcaatcc
     9061 gcagattcaa caatggctct tctctatcca gcgcgaattg ccgggcaatg ctgttctgga
     9121 ggtcaattat tcggccagca aggcaaccca taattacttt ggtggcgagg acctgcccgg
     9181 aaattacaat aagctgaatc ccgtctattg gggcctgggc cggaccgccc tggaatcgct
     9241 cgtgccgaac ccgtttcatg gaatcatcac cgatccaacc tctccggaaa gtctgccgac
     9301 cgtgccgctg aacgtgttgt tgcggcctta cccgcagtac gatggaggcc tcggagaata
     9361 taacgcccct ccgaatatcg ccaattcgat gtatcattcg atgcagctta aatacgagaa
     9421 gcggttttcg aagggtctgg cggtgctcgc cagttatacc atctccaagc tgatcagcga
     9481 cagcgacgaa tcctcgagcg atgtggactg gcagggcggc tcttcgggcg ttcagaacgc
     9541 atggaatctg cgcttagagc gctcgctctc tcaattcgac attcctcaaa gggcggtgat
     9601 cagcctggac tacgaactgc ccctgggccg cgggagaagg tttggaagca gcatgaacgg
     9661 cgtggcggaa gcgttggtcg gcggctggga ggtcgcgacg attcttacgt tcagttccgg
     9721 ttacccgatt gtgcctggct tggactcgcc gaatctttgg gacggagcgt cccaaaggcc
     9781 gaacatcatc ggtaatccat gcacatcggg ttcggtagaa tcgcgcatga acggatccta
     9841 cttcaacctg agcgccttct cacaaccgga tgaagatgtc ttcggcactg cgccgcgtac
     9901 gcttccggcg tgccgcacac cgggcatccg caacgcggac atcgtgctga tgaagaattt
     9961 tcgcttcaaa gagcgccagc agcttcagct ccggctggag tcgttcaacg tgaccaacac
    10021 cccgactttt gggcggcctg actccaacta cggttccaac acctttggcg tcatcagcgg
    10081 ctacgcctcc ggccgcggac cgcgggaaat gcaggtcgcc gccaaatttt atttttgaaa
    10141 gccgccggct gacggcgctg tgagtcccgg tgctctggcc gggattcagt cggcggatgc
    10201 cgcctgcttc gcgggacatg agggcggcca tcgatagcag agatgccggc cctacgcacg
    10261 cggagtaaag cgtaggcgcg cctccgaagg aacggttggg ccgccgcttt catccgggct
    10321 ttttcctgtt gtatgctaga cttgtcaagg agatttcctg gaggcaaagt atgcctaagt
    10381 ttcatggacg ccacctcttg ggcgtcgccg cattgctggt gtttttcgcg agcgtttccc
    10441 gggcgcaggt atccacgttg caaggggagg taaaggatga gaacggaaaa cctctccaaa
    10501 acgcaatggt ccacatcgag cgaaaagaca tcaaaggcaa ttaccaggta aagaccaaca
    10561 agaagggcca atacctccac gcaggcttgc cgctcggtgt gtataaggta tcggtcgaag
    10621 tgaacggcaa aaccatggac acggtggata acgtccgcac caaactcggc gacccgacca
    10681 cggtggattt caacctggcc gaggccaagg cgcgggcgga ggcggccgcg gcgggcatgg
    10741 aattgaatga gaaccgccag atgaccgcgg agcagcgccg gcagattgaa gaggccacca
    10801 agagacggca ggagcagctt agcaagaaca aagcgttgaa cgacgccttc aacgcgggca
    10861 tggaagcgat gcgcgccaaa aattgggaaa gcgccatcga ttcgctgact aaggcctccg
    10921 agatcgatcc caaacaacac gtagtctggg ccaatttggg cgaagcaacc atgaacctgg
    10981 cttccgccaa gcctccggct gagcagcccc cgttgatcgc gaaagccatg gaggattaca
    11041 ccaaggcgat cgagatcaag cctgatgatg ccgcttacca caacaattac ggcttggcgc
    11101 tggcgcgcgc cggcaagtac ccggaagccc aagccgagct cagcaaagca gccgagctgg
    11161 acaagccgaa tgcaggccgt tatttcttta atctgggagc gattttagtc aacaccggcc
    11221 acaacgacga ggcctatgag gcgttcaaga aggctgtcgc cgccgatcct aactatgctg
    11281 acgcatatta tcagatcggc gtgtacttac tgagcaaggc ccaagtagca gccgacggga
    11341 aggtcacgcc ggctccggga accgccgaag cattccagaa gtatttggaa ctggcgccga
    11401 acggccccaa tgccgcatcg gcaaaaggaa gcttgcaggc cattagcggc tcggtccaaa
    11461 cgcagtatgg aaccgagaag cccgctcctg cgcctgcggc aaagaagaag aggtaaatgc
    11521 gttttttact cggcctgatc gcgattctgg cagcgtttat actgttgcgg tcgctgctcg
    11581 aaccggtggt gaaggctgtg gtagccctgc tgacgccccc ggcggccacg catacagccg
    11641 cgggcgtcag atccggctcc gccccccgcg cgagcgaact caagaaagat ccggtttgcg
    11701 gcacatttgt ggccccggag ctcgctgtga cccaggaaat ccgcggtcaa atcatgcatt
    11761 tctgctcgcc aaagtgccgg gacgagtaca gccggcgagc ataatcccgt tttcacaccg
    11821 gaattcatag gaacggagcc gcggctcttt tttccgcagc cgggccggtt tgcatcgctt
    11881 agtttccgga tgtatgctgt ggtttgccat ccggtttcca cagaggttgc ttctatgtcc
    11941 gacatttccc gcaggcgatt taacgagacg cttgtttcag ggatcggagc gtctctggtc
    12001 acggcatcgc cgctgcaccc gcaagcctct tctgctaaga agcctaatat tctcttcatc
    12061 tgcgccgacc agcatagcgg actggtaatg gggccgaacg gccaccccat cgtccgcacg
    12121 cccaatctgg atcgccttgc cgcgcgaggc gtcaatttcc gcaacgctta ttgcggcagc
    12181 ccggtttgcg tgccctcccg ggcgggcctg atgacaggca tgtttccttc cgacgtaggc
    12241 tcgtactgca acagcacgca tttcgacggg cgcgtgccga cttggggcaa ccggctgcgc
    12301 gacgcaggtt atcactgctg ggcgacggga aaattggatt tggcgcccgg aaaggattac
    12361 ggctttcgcg aggtccacac cgccaacggc cacgccgagc atccggacat aacgtcactt
    12421 ttccgcgctc ccttatgttt tcgcgtcgac gagcggcggc agatcgatgg aaggtttcag
    12481 cagcgcgtac attccgacga tgcgcacgtg aaatacgctc tggattttct gcgcaacgag
    12541 gcgccccggc agcagaaccc gtgggctgct tacgtgggcc tcacgcttcc ccatccgagc
    12601 ttcgtcgcat tgccaaaata tgctgaaact taccggccgg aagacatgcc gttgcccaat
    12661 atccctgccg gttacctgga gcagcgccac attgtgttcc aggctttggc caatttcaag
    12721 ctgatttcga cgccgatccc ggaagcccgg gtacgccgcg cacgggccgc ttacttcggg
    12781 atgatcaccg agctggacgc gtatatcggc aatctgcttg acgagctgga aggatccggg
    12841 ctgctcgaca atacgcttgt ggtttatacc agcgatcacg gcgagatgct gggcgagcac
    12901 gggctatggc tgaaaaacaa cctgctcgaa ggctcggcgc gtgttcctct tatcatggcc
    12961 ggtcccggcc tgccgcgcgg ccgcacagtc gaaaccccgg tggcccatgt ggatcttgtg
    13021 gccacaatgc ttgatctggc cgcggcgtcc actgcgggag gtttgcgagg gcgctccctg
    13081 ctgcggctgg cccatggcca gccgggaaat catcctgggt acgctgtgag cgaatcgcat
    13141 tcagaaggca attgtacggg atcgttcatg gttcgcaaag acaactggaa gtatatttat
    13201 ttcagttggt atgagcccct gcttttcaat ttgaaggaag accccggaga gctgcgcaac
    13261 ctggccggca agccggaaac aacttccgtt cagaaggagc tgcacgggat tctgacctct
    13321 ctgcttgacc cggacgctgt cactgggcgc gctttcgccg agcaggagcg ccggtttttg
    13381 caaatggcga gagaacgtcc cgccgattcg ttctataagg agctcgccgg ccgcctcggt
    13441 cctggccagg cgagagctat taccaataag tactacaaaa atcgcaacgg ttgagagcgt
    13501 cgccgctccg cggtcccgtt ggctgttccg cgggaaaaaa ggcggggtga aacctgccgc
    13561 tccggccatc tcgcttaatg cggtaatggc acgcgcctgc cgatgcgctg cgctcacgaa
    13621 accttgcggg cgagcgactc atttctcgtg gcggagcggc cagggccgaa ggccagcgcc
    13681 agcagagcgg cttgctcgat cgcgtgcgcc tttgcggagc cggttgccgg gctggcgctc
    13741 gccgaacgct tcacggaacg gaacgggcgc ttccccgcga gacgccgcag ccgcggctgc
    13801 acgaaaaata aggcccccat gttggccggc tcttcctgca cccatacgat ctcggcgttg
    13861 gggtatcgat cgagctcgcg agcgagctcc tgttcgggga acggataaag ctgctccaga
    13921 aatagaatgg ccgtcgaaga atcgcctctt ctctgccgct cgctgcgcag ttcgtggccg
    13981 attttgcctg tgcacaggag cacgcggtcg ggttgctggc tttctgaatc ggcgatcacg
    14041 gtgagaaagc ggccttgcgt gaacgcctcg atcggcgagc acgccctggg atcgcggagc
    14101 atgctcttgg gggtgaaggc cacgagcggc ttgcgccacc gcctcacggc ctgccggcgg
    14161 agcaggtgaa agtactgcgc ggccgtagag ggctgcgcaa tttgcaaatt gtcctcgccg
    14221 gcaagctgca agaaccgctc gatgcgggcg cttgagtgct ccggtccttg gccctcgtag
    14281 ccgtgcggaa gaagc
//
LOCUS       DASREK010000105         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1379453, whole genome shotgun sequence.
ACCESSION   DASREK010000105 DASREK010000000
VERSION     DASREK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379453"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VFA54_05290"
     CDS             complement(<1..1355)
                     /locus_tag="VFA54_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZS50253.1"
                     /translation="MTDRTLYQVLDETARQYGDAPALRQPSPGGGYLTYSWNQYRRAA
                     EEIAAGLGTLGIRKGDIVALDSETRLEFYLADLGIVTAGAVAAALYPSYPPPDLIATV
                     AAIGAAVVFVEDRKTLQSLQGAAVRHWILLTGEAPTKNEDTLTLEALRERGRAALTED
                     PTLLERMRARVQPSDAAILYLTSGATGEPKKALVTHQAVVANIDMGPAALPLGPSDAT
                     VVFLPSAHIAQRVVMEFLPLRCGMPVTFSESLLRLPQEIRSVRPTILLAPPRMWERIY
                     ASICTELKKRPAAVRKAFYGALGLSLAAARYRRQGQAVPARIRFPLLVADRVLFRTVR
                     ARFGGRVRVAASGAAPLGKDLAEFYEAIGMPIIEGYGLTEGGVVTLNPLDAPQPGSIG
                     KPLPGIEVKLSDAGELLVKSPCLFSGYLNDEDSTARVLREGWLHTGDLASFDDRGYLY
                     IT"
     gene            1740..2024
                     /locus_tag="VFA54_05295"
     CDS             1740..2024
                     /locus_tag="VFA54_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HZS50254.1"
                     /translation="MDVNAIQQMMQPQVATAPVAPVQQPQQYREVIQSVKALNAAEMF
                     GQQNELMFHMDRQTRRMVIRVVNRETQEVVSQVPPEYILRLAEDLKAPAV"
BASE COUNT          429 a          631 c          738 g          368 t
ORIGIN      
        1 gtaatgtaga gatacccgcg atcgtcaaag gacgcgaggt caccggtatg cagccaaccc
       61 tcgcgcaaaa ctctggcggt agagtcctcg tcgttcagat agcccgagaa caggcacggg
      121 ctcttgacga ggagttcgcc ggcatccgag agcttcactt cgatgcccgg aagcggcttg
      181 ccgatgcttc cgggttgcgg agcatccagc ggattcagcg tgaccacccc gccttcggta
      241 agaccgtagc cctcgatgat cggcatgccg atggcttcat agaattcggc gagatccttt
      301 ccgagaggcg cggcgccgga ggcggcgacc cgcacccgtc ctccaaagcg cgctcgtacg
      361 gtgcgaaaca ggacgcggtc agcgaccaag agggggaagc ggatacgggc tggaacagct
      421 tgtccttgcc ggcggtagcg cgcagccgcc agagagagtc ctaaagcgcc gtagaacgcc
      481 ttgcgcacgg cggccggccg cttctttagt tcggtgcaga tgctggcgta aatccgctcc
      541 cacatgcgcg gcggcgccag cagaatggtc ggccggacgg agcgtatttc ctgcggtagc
      601 cgcaacaggc tttccgagaa cgtcacgggc ataccgcagc ggagcggaag aaactccatc
      661 acgacgcgct gcgcgatatg cgccgacgga agaaacacca cggtggcatc gctcgggccg
      721 agcgggagtg ccgccggacc catatcgata ttcgccacaa ccgcctggtg cgtgacgagg
      781 gctttcttcg gctcgccggt cgcgccggaa gtcaagtaca ggatagccgc atcggaaggc
      841 tggacgcgcg cccgcatgcg ttcgagcagc gttgggtctt cggtgagcgc ggcgcgacct
      901 cgctcgcgca aagcctcgag ggttagggtg tcctcgtttt ttgttggagc ttctccggta
      961 agcagaatcc agtgccggac cgcagcgccc tgcagggact ggagcgtttt gcggtcttcc
     1021 acgaagacca ccgccgctcc gatagctgcc acggtggcga tgagatcggg cggcgggtag
     1081 ctgggataaa gcgcagctgc cactgcgccc gcagtcacga tgccgagatc ggccaggtaa
     1141 aattccaggc gggtttccga gtccagggcg acgatgtcac ctttgcggat gccgagcgtg
     1201 ccgagcccgg cggcgatttc ctcggcagca cgccggtact gattccagga ataggtgaga
     1261 tagcccccgc ccggggacgg ttggcgcaag gccggcgcgt cgccatattg gcgcgcggtc
     1321 tcgtcgagaa cctgatagag agtccggtcg gtcacccctg caatttaaca tggacttttt
     1381 ttgcagggct gttcgcgaga gcggctgggg agggcaaggg gcgaggcgcc ggcgcactcg
     1441 gcggcccgcg agacataaag caaggcttgg cgggccagtt ctgggaccgt gggatcggga
     1501 tggcgcagaa gggcaaccag tttccggatc caggggagct cggcggggga cagaccaatc
     1561 atgggcacgg tgccggccat cgaaactctg cctctgattc ccttttcggc caaggggcgg
     1621 aaatcgtgag cggctggccg cggtaaagcg cctcaagcgt ttgccggctc cgccgataag
     1681 ttgggttgag gaagcgggcc gagccggcaa tccggcgccg cccgttcgaa ggagccgtta
     1741 tggatgtgaa cgcaatccag caaatgatgc agccccaggt ggcaacggca cctgtagcac
     1801 cggtacagca accgcagcaa taccgcgaag tcattcagtc ggtgaaggca ttgaatgccg
     1861 ccgaaatgtt cgggcagcag aacgagttga tgtttcatat ggaccggcag actcgccgga
     1921 tggtgatccg ggtcgtcaat cgcgagacgc aggaagtcgt gtctcaagtc cccccggaat
     1981 atattttgcg gctggctgag gatctgaagg cgcccgccgt ctgatcgagt ctggatagtc
     2041 ggagttgcgg aaaccgcggg acgatcatgg ccgcgcgcga gaaccggcgc ggggtgaaaa
     2101 acgaagatgt ggaacactgc gcaagacgca tacttggaga atcggattct gtccgccgat
     2161 ccgctg
//
LOCUS       DASREK010000106        10848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_58419, whole genome shotgun sequence.
ACCESSION   DASREK010000106 DASREK010000000
VERSION     DASREK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58419"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1790
                     /locus_tag="VFA54_05300"
     CDS             <1..1790
                     /locus_tag="VFA54_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50255.1"
                     /translation="STANSLAVKGGSITASGGIVVDARASPPHVYIADTPNNRVLAWR
                     NLNSLREGSRADIVLGQPDLETTLSNYGSPAAGDRPASPSNLNRPSGLALDPEGNLFV
                     ADTGNNRVLRFPRPFDSPGAAADLVIGQPALSGYSVQGASAFLLNHPTGIAVEPLRGD
                     LAVADTLNHRVLLFAAPLRSAMPASQVLGQSSFVDSAPGLSSTALSFPTGVAFDSQGD
                     IYVADTGNSRIMVFAPPRELPATGAAALASGPAPLGQPDFVTGVGGTAGNRLRNPTGI
                     VVNSRDGDLWVADTGNHRVLRFPPLPVLLTNGGAPYASGGLIGQMSFTGRTENLGAVL
                     SGQTSPAGLSFPNAVALDDWGNLLVGDGNARVLLYFPQASVVSAATYWAGVPLAPGML
                     ASIFGDQLTAETALSGIPLATTLADTQVWVNGVPAPLLYVSPGQINFQMPSNVTPGTA
                     VRLEVVRASSGQTIAGGSAAIAPAAAGIFGVLNEDNSPNSPANPAARGSIIQIFATGQ
                     GPVSGGPGDGSPAPDSPLATTPSTPLVTIGASSQRDVFPDFSGLAPGFVGLWQINARV
                     PAPTAPGPQVPVIVRYGGSASNVVFIGVK"
     gene            1844..2398
                     /locus_tag="VFA54_05305"
     CDS             1844..2398
                     /locus_tag="VFA54_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZS50256.1"
                     /translation="MQLEFAIALGLMLAAETSAPKWNPESYIIGPGDVLTVQVWKEPD
                     LSRRVPVRPDGRISLPLLNDVQAAGLTPNELAGRVSQELKKYLTEADVAIVVEQVNSR
                     RIYVMGEIARPGAYPLLAQTSVLQALSAAGGFREFANTKKIYILRGAGVYQKRLLFDY
                     NAVVKGKKPEQNILLENGDTIVVP"
     gene            2408..3730
                     /locus_tag="VFA54_05310"
     CDS             2408..3730
                     /locus_tag="VFA54_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50257.1"
                     /translation="MRNRCLCAVAFLPAILCWGQTPEAPALLSRGVAAESGLPRAGVL
                     QPYAGAFSGYDNGPLSTSLDRPGGAFQEVEGGLSFLRRGTRAEIRLDYRFDLRHYQAS
                     SRLDRSNQALTVDTRFHISRRWTVMLRDTGSSASYDSPSQPAGTGVAEGFLVGVGPEV
                     FHSRTIAQTGMADVIFAPTARTSVSVGGDGFLIQRQFAPLIDAVGWRARADVARRYSR
                     HRTVSLSYSFTHFDHARSFGGAEYAVFALGHSVRLSRRSEIDLLAGAGRLQSAGLRAV
                     SLDPEISRLLGISRGVETFHLDTWAPHVTAAWVQGVGRAELRVQFAREVTDGGGLTGL
                     ARQNEGSLAFQTAHVRSAWRPGIHIIVRTYRSLDTILYSNVTASAGLSLARRLGSQTE
                     AVVRYDFGFYNFEEGLLRNFQRRQFAAGVTCYFSGRATPPNFDRAIRP"
     gene            3925..4368
                     /locus_tag="VFA54_05315"
     CDS             3925..4368
                     /locus_tag="VFA54_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS50258.1"
                     /translation="MRYFLLLTAVLVTPALVSAAEGSSTPATYISADKVTAGFGKGGF
                     LARTDEYNVQTSRRVKPGNPEVHLKDTEIMYIVNGSATLVTGGALVDGKTTRPEQMVG
                     TSVNGGTSQKISKGDVIVVPAGTPHWFQEVNGTVDYYTVKVHSSK"
     gene            5058..8507
                     /locus_tag="VFA54_05320"
     CDS             5058..8507
                     /locus_tag="VFA54_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50259.1"
                     /translation="MFRAPCLVLIGTIFFCARVLAQSDNAAISGFVRDPQNAVVPNAT
                     VVVKNEANGVERRTATNESGYYIVSNIPPATYTVRVEAKGFRVFEQAGNKLNPNINTS
                     VDVTLQVGAVTETVEVTASVATLQSESAVIGKLVDRTQIDALELNGRNPIFMAQLVPG
                     ARGTTLANLNFNFSQGPANFNGSRNPENLITIDGAPATRTRSNGTSLGSADVDSVQEI
                     QVLTANYAAEYGRSSGGQIRIITKAGGREFHGSAYEYLRNTALNANTWQRNTNPSTGF
                     TAPIHYNQFGYNVGGPFYIPRRFNTDKSKFFWYWGQEWVRYRFTDSNTLTVPSLLMRQ
                     GDFSELLDPKNFFYGKTVIIKDPSNGQPFPNNIIPKSQLSPNGIGILNAWPAPNLATP
                     INGNRNWYKAAIHPQNQRKDTLAADMNLTDRQRLTFRRLNYAFNEYQPLDGGTDRTPK
                     FFNRPNQTNSLNWVWTISPTLVNEALATVSLDDVYIPVDTAHFFDRTQAGINYPYIFP
                     EGKLIPTRIPTVNMSPFSQLSGGPYPSHSSGPIYDVSDSLTWIKGSHTLKFGFLYERS
                     GENDNDEINVSGCPTCTNNQNGQFLFTDARSGFPSTGAAVANAALGLFDTYSEIGHRA
                     YTIFRGSMNEAFVQDSWKATQKLHLDYGVRYTVIVPYHALWGNMIVFDRSFYDPSKAV
                     KVDPKTGFIIPGSGDPYNGMVIPGSGFPGSAKGRFPEADTNQFNYLFRGVNSHYSDIQ
                     WGDVQPRVGVAYQLNQKTVVRAGIGRFFTRLGVSDSIFLGGNPPFQPNANVSFGNVDN
                     PGGTSTNAVPLVVTTQSKAFKNPEAWNWNVTVERQMPWSTVLSVAYVGRRGLHLQREA
                     DINQPPAGTLQANPGVNLDALRPYKGFGSIRETDNVASSRYNSFQLNWTRRFSNGLLF
                     GVAYTLSKSWDDGSNQRDIIPDTYDAHMLWGPSEFDTRHIVAINYLYELPFFRGRSNL
                     ASKLIGGWQISGITQFQTGLPCGVAGSTDYAGTGQDGNFGCGVSGQYWVVKGTPTVTG
                     QFAARGSQDPAQWFTVKNADGSAIFTPPPAGTFNTQRVRNLLYGPGYQNWNLGLFKRF
                     PINERTGLQFRAEAFNFINHPNWGNSNNVNSVDYNPTSSTFGKVTNKYSERNIQLSLR
                     YYF"
     gene            8641..8728
                     /locus_tag="VFA54_05325"
     tRNA            8641..8728
                     /locus_tag="VFA54_05325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8674..8676,aa:Leu,seq:taa)
     gene            complement(8731..10119)
                     /gene="accC"
                     /locus_tag="VFA54_05330"
     CDS             complement(8731..10119)
                     /gene="accC"
                     /locus_tag="VFA54_05330"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZS50260.1"
                     /translation="MFKKVLIANRGEIAVRVIAACKELGIRTVAVYSEADRASLHVRF
                     ADEALCIGPAKSADSYLNIPAVISAAEITDVDAIHPGYGFLSENPGFAEVCEMSGITF
                     IGPTPETLRLMGEKEHAREAMAKAGLPILPGSDGLLKNEEHALAAAREIGYPVIIKAA
                     NGGGGRGMRIVREPRDLGPMLHQAQHEAAVSFQSADVYLEKYIQRPRHIEFQVLGDAE
                     GNVVVLGERECTIQRRHQKLIEESPSPRVDAATRSRMGQRIARALQNIGYCNAGTVEF
                     LMDEDGSLYFIEMNTRIQVEHPVTEQVTGIDLVKAQILIAGGEPLSSILSEPVEIRGH
                     AIECRINAENPQTFAPSAGRITKFHAPGMAGIRVDTAAHADCFVPPYYDSLVAKLIAS
                     GRDRAEAITRMQRALDMFIIEGIYTSIPIHQQIMADADFVLGNFDTTYINRLIQQQKV
                     EAATQPAAESGR"
     gene            complement(10131..10628)
                     /gene="accB"
                     /locus_tag="VFA54_05335"
     CDS             complement(10131..10628)
                     /gene="accB"
                     /locus_tag="VFA54_05335"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZS50261.1"
                     /translation="MNLKEIKELIDTLTEKDVAEFELERGNEKIRIKRAVAADGAAHI
                     AFASAAPSVPAAAAPPPAAAPSAAVPAAAPPPAASQTEEELHIIKSPIVGTFYESSAP
                     GAPPLVEIGDTVQVGQVLCIIEAMKLMNEIESDVAGIVEKRFVSNQQPVEYGEALFGI
                     RKKNP"
     gene            complement(10625..>10848)
                     /locus_tag="VFA54_05340"
     CDS             complement(10625..>10848)
                     /locus_tag="VFA54_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZS50262.1"
                     /translation="FVHSTGHGVGLEIHEAPRLGKNDKTLLESGMVITIEPGAYLEDL
                     GGVRIEDMVLVKANGCEVLTPTSKDLRIL"
BASE COUNT         2051 a         3404 c         3183 g         2210 t
ORIGIN      
        1 tttctactgc caattctttg gctgtcaaag gcggctctat caccgcctcc ggcgggatcg
       61 tcgtagatgc ccgcgcctcg ccgccgcacg tctatatcgc agacactccc aacaaccgcg
      121 tgcttgcctg gcgaaatctg aactcgctcc gtgaggggtc gcgcgccgac atcgttctgg
      181 gacagcctga tttggaaacg actctctcca actatggctc cccagctgcc ggggaccgcc
      241 cggcaagtcc gtcgaacttg aatcgccctt cgggcctggc tctggacccg gagggaaatc
      301 ttttcgtcgc cgacaccggc aacaaccggg tgctccgctt cccgcgcccg ttcgacagcc
      361 cgggcgccgc ggcggatctc gttatcggcc agccggcatt gtcaggctac tccgtacagg
      421 gcgcttcggc ctttttgctg aatcacccca ccggaatcgc tgtggaaccg ctccgtggag
      481 atctggccgt cgccgacaca ctgaatcacc gtgtgcttct gttcgcagca ccccttcgaa
      541 gcgcaatgcc ggcttctcag gttcttgggc agagcagctt tgttgacagc gctcccggtc
      601 tgtcgagcac cgcgctgagt tttcccacgg gggtagcgtt cgacagccag ggggatatct
      661 atgtcgccga taccggcaac agccgcatta tggtgtttgc gccaccccgc gagctgccgg
      721 ccactggagc ggccgctctt gccagcggtc cggcgccgct gggccagccc gatttcgtga
      781 cgggtgttgg tggaacagcg ggcaaccgcc tgcgaaatcc gacgggaatc gttgtcaaca
      841 gccgggacgg cgacctctgg gttgctgata caggcaatca tcgggtgctt cgatttccgc
      901 cgctgcccgt gctgctgacc aacggcggcg ctccctatgc ttcgggcggg ctgatcggcc
      961 agatgagttt tacagggcgg acggaaaacc tgggagccgt cctctccggg caaacttctc
     1021 cggcgggact gagcttcccc aacgcggttg cgctcgatga ctggggcaat ctgcttgtgg
     1081 gggatggcaa cgcccgggta ttgctttact tcccacaagc ctccgtggtg agcgcggcga
     1141 cttattgggc cggagtcccc ctcgcacccg gcatgttggc atccatcttc ggcgaccagc
     1201 ttaccgcaga aacagccctt tctggaattc ctctggcgac gactttggca gacacgcagg
     1261 tgtgggtaaa cggcgtgccg gctccccttc tctatgtctc gcccggacag atcaatttcc
     1321 agatgccgtc aaacgtcacg ccgggaaccg cagtccggct ggaagtcgtc cgcgcctctt
     1381 ccggccagac catcgccgga ggatctgcgg cgatcgcccc ggcggctgcc ggcatttttg
     1441 gcgtgctcaa tgaagataat tcgcccaaca gcccggccaa tcccgcggcg cgcggctcga
     1501 tcatccagat tttcgccacg ggccaaggac cggtgagcgg cggaccgggt gacggaagcc
     1561 ccgcgccgga ttcccccctg gcgacgactc catcaacgcc tctggtgacg atcggcgcct
     1621 ccagccagcg cgacgtgttc cccgacttta gcggcttggc gccgggattc gtcgggctgt
     1681 ggcaaatcaa tgcccgcgta cccgcaccca cggcgcccgg cccacaggta cccgtgattg
     1741 tgaggtacgg aggctcggcg agcaatgttg tttttatcgg cgtcaaatga caatgcgcaa
     1801 agctgagtcg ccgcaagctc aagtgcggct taaggagtga tacatgcaac tggagtttgc
     1861 aatcgccctc gggttgatgc ttgccgcaga aacttctgcg ccgaagtgga acccggaaag
     1921 ttacatcatc ggccccggag atgtgctcac cgtacaggtc tggaaggagc cggatctttc
     1981 gcggcgggtg ccagtccggc cagacgggag aatctctctg ccgctcctca atgatgtgca
     2041 ggccgccggc ctgaccccta acgaactggc aggccgcgtg agtcaagagc tgaagaaata
     2101 tctcaccgaa gctgacgtcg ccatagtcgt ggaacaagtg aacagccggc ggatctacgt
     2161 catgggcgag atcgcccggc cgggcgccta tcccctgctg gcccagacct cggtgttgca
     2221 ggccctgagc gcggccggcg gtttccggga gttcgccaac acgaagaaga tttacatcct
     2281 gcgaggggct ggtgtttatc agaagagact tctattcgac tataacgcag tcgtgaaggg
     2341 caaaaagccc gagcagaaca ttctgctcga aaacggggac accattgtgg tcccgtgagg
     2401 ccgggatatg cgcaaccgct gcttgtgcgc cgtcgcattt ttgccggcga ttctctgctg
     2461 gggacagacg ccggaagctc ccgcactgtt gagccgggga gtggctgcgg aatccggctt
     2521 gccgcgcgcc ggcgtgcttc agccctatgc cggagctttt tcgggctacg acaacggacc
     2581 gctttcgacg agcctggatc ggccgggcgg cgcattccag gaagtggagg gaggtctcag
     2641 ttttttgcgg cgcggtacgc gtgccgagat tcgcttggat taccgctttg acctgcggca
     2701 ctatcaggcc agcagccgcc tggatcgctc gaaccaggcc ctgaccgtgg atactcgctt
     2761 ccacatttcg cgccgctgga ccgttatgct gcgcgataca ggctccagcg cctcttatga
     2821 cagccccagc cagccggccg ggaccggcgt agcggagggg tttttggttg gcgttgggcc
     2881 tgaagttttc cactcccgca ccatagcgca gaccgggatg gcagacgtaa tctttgcgcc
     2941 aacagccagg acctcggtat ccgtcggcgg cgatggcttc ctcatacagc ggcaattcgc
     3001 gcctttgatt gatgcggtgg gctggcgcgc gcgcgcagac gtggcccgcc gctacagccg
     3061 gcatcgaact gtctcgctct cttacagctt cacccatttc gatcacgccc gaagcttcgg
     3121 cggggccgag tacgccgttt ttgctttagg gcattcggtc cgcttgagcc ggcgctccga
     3181 aatcgatctg ctggccggag ccgggcggct gcaaagcgcc gggctgcgag ccgtatcgct
     3241 cgatccggaa atctcccgcc tgctcggcat ctcccgcggc gtcgagacct ttcatctcga
     3301 cacctgggcg ccccatgtga cggcggcctg ggtccaggga gtgggccgcg ccgagttgcg
     3361 ggtgcagttc gccagggaag taactgatgg cggcgggttg accggcctgg cccggcagaa
     3421 cgagggaagc ctggcgtttc aaaccgcgca tgtccgctcg gcctggaggc cgggcatcca
     3481 tattatcgtg cgcacctacc gctctcttga cacaattctt tactcgaacg tcacagccag
     3541 cgccggcctc tcgctggccc gccggctggg gtcacagaca gaagccgtgg tccggtacga
     3601 cttcgggttt tacaacttcg aagaagggct gctgcgaaat ttccagcggc gccagttcgc
     3661 cgcgggggtc acctgttatt tcagcggaag agcgaccccg ccgaatttcg accgcgcgat
     3721 ccggccctga actgcgtctt gcgatctgcc gcgtccgagt tgggcaatat cactgcgggc
     3781 tgctccgcgc tgaggcaggc gccggggact gcgcgcggct tgctccggaa cccgagaaaa
     3841 caaaaattcg atagactcgc cggagagcat tcaggtatga tactcggcac ggttgggtta
     3901 gcgaacgaat caaggagaca acaaatgcgg tatttcttat tgcttacggc ggtgctggtg
     3961 acgccggccc tggtcagcgc tgccgagggg tcctcgactc ccgccactta catcagcgcg
     4021 gataaggtga cggcgggctt cggcaaaggc ggtttcctcg ccaggacgga cgagtacaac
     4081 gttcagacga gccggcgggt gaagcccggc aatccagaag tgcatctcaa ggacaccgaa
     4141 attatgtaca ttgtcaatgg ctcggcgacc ctggtgaccg gcggcgcgtt ggttgacggc
     4201 aagaccaccc ggccggaaca gatggttggc acgagcgtga acggcggaac gagccagaag
     4261 atcagcaagg gcgatgtgat cgttgttccg gccggaactc cgcactggtt tcaggaagtc
     4321 aacgggacgg tcgattacta cacagtcaaa gttcactctt ccaagtagcc gttgaccgcc
     4381 cgaactgcat tttgggagcg gcgcctgccg cttccaaaat agcagtttgc gaccgttgcc
     4441 accaccaacc cgaccacatg cgcgcccttg ttgatgcacg ccgggccgcg ctatttgcgt
     4501 catttaatgt cgtgggtcgc ggctggcagg ctgttgcaca aagccggcgg gaaggccgca
     4561 tggtgagctt ggagggggcg gtttttcatc gagatttcct gccaactttt cagattctca
     4621 atcggaatag ttgttaaacg ccggaagaat tcctgacctc aatttccctc gtattccgcc
     4681 ataaccggct tttgaggcgg gcgtgttcgc gggcatcagg cccgcaaggg cggcgtgggc
     4741 cccggccatt cgaaacaaat tcacaaaatt ttcttgacaa agagcggctt ttcatgttat
     4801 cgcttcttgg caagggtttt ccggcaacaa cgtttgcaat cgggattggc tctctggaaa
     4861 gggtggtttc gcggcccgtc ggcttccggc ggtcggccgt tctagggggg ctaggatgag
     4921 agtcctgatt cggcgcccgg gcgtggcgcc gtccatttat ataccaattg gggccaggat
     4981 ctcagcccat ccagttttga gcccaatcca attttgcctc cggtctgccc cgctgccata
     5041 agcggaaagg agggtttatg tttcgtgctc cgtgtctcgt gctgatcggc acaatcttct
     5101 tttgcgccag ggtgctggcg cagtccgaca acgccgcgat ttccggtttc gttcgtgacc
     5161 cgcaaaacgc tgtggttccc aacgccacgg ttgtcgtcaa aaacgaggcg aacggcgtgg
     5221 agcgccgcac ggccacaaat gaatccggtt attacatcgt tagcaacatt cctccagcca
     5281 cttacacggt tcgcgtggag gccaagggct tccgcgtgtt cgagcaggcc ggcaacaagc
     5341 tgaacccgaa tatcaacacc tcggtggatg tgacgctgca agtgggggcg gtaacggaga
     5401 ccgtcgaggt gactgcctcg gtggccacgc tgcaatcgga aagcgccgtg atcggcaagc
     5461 tcgtggaccg cactcagatc gatgcgctcg agctcaatgg ccgcaatccg atcttcatgg
     5521 cacagctcgt gccgggcgcc cgcggcacca cgctggccaa cctgaacttc aatttttccc
     5581 aggggccggc caactttaac ggctcacgta acccagagaa cctgattacc attgacggcg
     5641 ccccggccac gcgaacccgt tccaatggca ccagccttgg ctcagccgat gtggactctg
     5701 tgcaggaaat tcaggtgctt acggcgaatt atgcggcgga atacggccgt tcgtccggcg
     5761 gccagatccg catcattacc aaggcaggcg gccgcgagtt tcacggctcc gcttacgaat
     5821 atctgcgcaa cacggcgctc aacgccaata cctggcagcg caataccaac cccagcacgg
     5881 gctttacggc tccgattcat tacaaccagt tcgggtataa cgtcggcggc cctttctaca
     5941 ttccccgccg attcaacacc gataaaagca agttcttttg gtattgggga caggaatggg
     6001 ttcgataccg ctttaccgac tcgaacaccc taaccgttcc atcgctcctg atgcggcagg
     6061 gtgatttcag cgagctgctg gatcccaaga acttctttta cggcaagacc gtcatcatca
     6121 aagatccttc gaacggccag ccgtttccca acaacattat tccgaagagc cagctcagcc
     6181 cgaacggtat cggtattctg aatgcttggc cggcgcccaa cctagccacg cccatcaatg
     6241 gcaaccgaaa ctggtacaaa gcggcgattc acccccagaa ccagcgtaaa gataccctgg
     6301 ccgccgacat gaacctcacc gaccggcagc ggctcacatt ccggcgtctc aactatgcgt
     6361 tcaatgagta ccagcccctg gatggcggca cggaccgcac gcccaaattc ttcaaccgcc
     6421 cgaaccagac caattcgctg aattgggttt ggaccatcag ccccacattg gtcaacgaag
     6481 cgctcgccac tgtgagtctg gacgacgttt atattcctgt ggacacggcc cactttttcg
     6541 accgcaccca ggcaggcatt aattaccctt acatattccc ggagggaaag ctgatcccga
     6601 cgcgcatccc cacggtgaat atgtccccct tctcccagct cagcggcggg ccctaccctt
     6661 cgcactcctc cggtcccatc tacgatgtgt ccgacagcct gacctggatc aagggcagtc
     6721 acacactgaa gttcggtttt ctttatgagc gctcggggga aaacgacaac gacgagatca
     6781 acgtaagcgg ttgtcccacg tgtacgaaca accagaacgg ccagttcctt ttcacagatg
     6841 cgcgatcagg attcccctcc acgggcgcgg ctgtggcaaa cgccgctctg ggcctattcg
     6901 atacgtattc ggagataggc catcgtgctt atacgatctt ccggggcagc atgaacgagg
     6961 cttttgtaca ggattcctgg aaggctacgc aaaagctgca cctcgactac ggcgtccgct
     7021 atacggtcat cgttccctat cacgccctgt ggggcaacat gatcgtgttt gacaggtcgt
     7081 tctacgatcc cagcaaagcc gtgaaggtcg atcccaagac gggctttatc attccgggtt
     7141 cgggagatcc ttacaatggc atggtcattc cgggcagcgg cttccccgga tccgcgaagg
     7201 gacgtttccc ggaggcggac acgaaccagt tcaactactt gttccgcggc gtgaattccc
     7261 actattccga catccagtgg ggcgatgttc agccgcgcgt cggcgtcgct taccagctca
     7321 atcagaaaac cgtagtgcgc gccggcatcg gccgcttctt cacccggctt ggcgtcagcg
     7381 attctatctt ccttggcggc aacccgcctt tccagcccaa cgccaacgta tccttcggca
     7441 acgtcgataa tcccggcgga acctccacaa acgccgttcc cctggtcgtg accacgcaga
     7501 gcaaggcttt caagaatcct gaggcctgga actggaacgt cacggtggaa aggcaaatgc
     7561 cctggagcac ggtgctgtct gtggcgtacg tgggccgccg agggctacac ctccagcggg
     7621 aagccgacat caaccagccg cctgcgggca cgctccaggc gaatcccggc gtcaacctcg
     7681 acgcgttgcg gccgtataaa ggcttcggct ccattcgcga aacggacaac gtggccagtt
     7741 cccggtacaa ctccttccag ttgaactgga cccgccgctt ctcaaacggg cttttgttcg
     7801 gcgtcgcgta cacgctttcg aagagctggg acgacggctc caaccagcgc gatatcattc
     7861 ctgataccta cgacgcccat atgctgtggg gaccgtcgga gttcgacacc cgccacattg
     7921 tggccattaa ttacctctat gagctcccct ttttccgcgg gcgctccaat ctggccagca
     7981 agctgatcgg cggctggcag atcagcggca tcacccagtt ccagaccggt ttgccttgcg
     8041 gcgtggccgg cagcaccgat tacgccggta cggggcagga cgggaacttc ggatgcgggg
     8101 tcagcggcca gtactgggta gtgaaaggaa cccccaccgt caccggtcaa ttcgcggccc
     8161 gcggttcgca ggacccagct cagtggttca ccgtgaagaa tgcagacggc tcggcgatct
     8221 tcaccccgcc cccggcgggg acgttcaaca cgcagcgggt ccgcaacttg ctttatggtc
     8281 ccggctacca gaactggaac ctggggctgt tcaagaggtt tccgattaac gagcggaccg
     8341 gcttgcagtt ccgcgccgag gcgttcaatt tcatcaacca cccgaactgg ggcaactcga
     8401 acaacgtgaa cagcgtggac tacaatccga ccagttcgac gtttggtaag gtgaccaaca
     8461 agtacagcga gcgcaacatc cagctttcgc tgcgttatta cttctaacca agctgccggc
     8521 ggccgagcgc atcagcgccc ggccgctgcc ttccctgccg tttttcagag tctttcgatc
     8581 acctccggtg ggtcatcacc ggcatcccga ggggagtttg ggctaagctg ttggtagcgc
     8641 gcccgggtgg tggaatggca gacacaaagg acttaaaatc ctttgggccg caaggtccgt
     8701 gcgggttcga gtcccgcccc gggcaccagc tcaacgcccc gattcggcgg cgggctgtgt
     8761 ggccgcctcg accttttgct gctggatcag cctgttgata taggttgtgt cgaaatttcc
     8821 cagtacgaaa tcggcgtccg ccatgatttg ctggtgaatc ggaatggatg tgtagatccc
     8881 ctcgatgatg aacatatcga gagcgcgctg catgcgcgtg atggcctcgg cgcgatcgcg
     8941 gccggaggcg atcagctttg cgaccaagga atcatagtac gggggcacga aacagtcggc
     9001 gtgggcggcc gtatccacgc gaatgcccgc catacccggc gcgtggaatt tcgtgatccg
     9061 gccggccgaa ggggcaaacg tctgcggatt ttccgcattg attcggcact cgattgcgtg
     9121 gcccctgatt tctaccggct cgctcaggat ggaagaaagg ggctcgccgc ccgcgatcag
     9181 tatctgcgcc ttgactaaat ctatgcccgt cacctgctcg gtcaccggat gctccacctg
     9241 aatgcgggta ttcatttcga tgaagtacaa gctgccgtct tcatccatca gaaattccac
     9301 cgtgccggcg ttgcagtaac cgatgttctg aagcgcgcgg gcgattcgct ggcccatccg
     9361 gctgcgcgtg gcggcgtcca cgcggggcga cggcgactct tcgatcaact tctggtgccg
     9421 ccgctggatc gtgcactccc gctcgccgag cacgacgacg ttgccttcgg catcccccag
     9481 cacttgaaac tcgatgtgcc ggggccgctg gatgtatttt tccagataga catcagccga
     9541 ctggaagctc accgccgcct catgctgcgc ctggtggagc atgggaccca ggtcccgggg
     9601 ctcgcgcacg attcgcatgc cgcggcctcc tccaccgttg gcggccttga tgatgacggg
     9661 atatccgatt tcccgggcgg ccgccagcgc gtgctcctcg tttttcagca ggccatccga
     9721 tcccggcaaa atcggcaggc cggccttcgc catcgcctcc cgggcgtgct ccttctcgcc
     9781 catgagccgc agagtctcgg gcgtcgggcc aatgaaggtg atccccgaca tctcgcagac
     9841 ctccgcaaaa cccgggttct cgctcagaaa gccgtacccg ggatggatgg cgtccacatc
     9901 ggtgatctcc gccgcactga tcaccgcggg aatgttcagg taactgtcgg cgcttttggc
     9961 cggcccgatg cagagcgctt cgtcggcgaa acggacgtgc agagaggccc ggtccgcctc
    10021 ggaatacacc gcgaccgtcc ggatgcccag ctctttgcag gctgcgatca ctcgcactgc
    10081 gatctctccc cggttggcta tcagaacttt cttgaacata cgattcgccc tcaggggttc
    10141 ttctttcgga tgccgaacag cgcttcgccg tactcgaccg gttgttggtt ggatacaaag
    10201 cgcttttcca ctatcccggc gacatccgat tcgatctcgt tcattaactt cattgcttca
    10261 atgatgcaga gtacctggcc tacttgaacc gtatcgccta tttccacgag gggcggcgct
    10321 cccggcgccg acgactcata aaaagtcccg acgatgggag atttgatgat gtgcagctct
    10381 tcctcggtct ggctcgccgc cggaggaggc gccgctgctg gcactgccgc gctgggcgcg
    10441 gccgcaggcg gcggagcagc ggctgcggga acgcttggtg cggccgaagc aaaagcgata
    10501 tgcgccgcgc cgtctgccgc tacggctcgc ttgatacgaa tcttctcatt tccccgttcg
    10561 agctcaaatt cggcgacatc cttttcggtg agtgtatcta tgagttcctt gatctctttc
    10621 aggttcatag aatccgcaga tctttggacg tcggagtgag aacttcgcaa ccgtttgcct
    10681 tgaccaaaac catatcctcg atcctcacgc cgcccaagtc ctccaggtat gctcccggct
    10741 cgatcgtaat caccatgccg gattcgagca aggtcttgtc atttttgccg agccgcggcg
    10801 cctcgtggat ctccaggccc acaccgtgcc cggtggaatg cacaaaag
//
LOCUS       DASREK010000107        13814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2037631, whole genome shotgun sequence.
ACCESSION   DASREK010000107 DASREK010000000
VERSION     DASREK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037631"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VFA54_05345"
     CDS             complement(<1..883)
                     /locus_tag="VFA54_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZS50263.1"
                     /translation="MDRRSLLRLGGLSALSLIRLPPLAFAQEKSKLKITGVRLVKTRP
                     KRPLPPYTPAPGAWSTNGVEVASPMSIYPEYKAKRSLFLPDPDKVPSFTVEISTDKGV
                     KGYGSGGPGGGYVVEQHFTKLLLGEDPFNVERIWDILWRSSMSYGRAGLVIHGISGVD
                     LALWDLIGNALGMPVYKLLGGETKSRIPAYCTGNDIEQHIQFGYKRLKLAIPFGPADG
                     REGMKKNVELVQSTRKALGPDGDIMLDCWMSWNERYTIEMAEMVAPYRVYWMEECLPP
                     HDYEGFGRLNAAIKSTRI"
     gene            complement(933..3296)
                     /locus_tag="VFA54_05350"
     CDS             complement(933..3296)
                     /locus_tag="VFA54_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZS50264.1"
                     /translation="MQNTWTLLAGISAALAVCLPNPAAQPAASPAPARDAPAQFRQLC
                     SGCHGDKAAGTERGPALADNRSLRRRSEKQIQDLIRNGAPPGMPPFRLPENELEALAA
                     WVHSLNASVYDLRLPGDPAAGERFFFGKGQCASCHMVRGRGRTNGPDLSDIGRQLTLA
                     ELEQALDDPSARAGSRSSGSCPPWAWCPQDTWSVARVRLRNGSVLRGFARNQGKHDLQ
                     LQTFDGWMHLLLDTEYDEVSREKGTVMPALHSTAEERRNLIAYLARLGGVPTGPLTGE
                     FEPAGAGAMESVLRPPPGDWPTYNGDIRGNRHSALRQITTENANRLQLQWTYTLPYIG
                     LEMTPVVSGGVMYVSAPNRICALDSRTGREIWCYSRPRSAADTIAGDAAKGANRGVAV
                     LGDWVYFATDNAHLICLHRLTGALIWDVVMPEGPGRYGATAAPLVVGDLVISGVSGGD
                     GPLRGFLAAYNAGTGRQVWRFWTVPQRGQPGSETWRGSALETGGGATWLTGSYDPETG
                     LLFWPTGNPFPDTDGDQRAGDNLYTNCVLALEAKTGKLRWYYQFTPHDLHDWDATEPL
                     VLVNAMFHGRERKLLLQANRSGFFYVLDRTTGEFLLGKPFVRRLTWASGIGAGGRPHV
                     VEGNKPTPAGTKTCPAVRGATNWYATTFNPETKLFYVMAIEDCNIYRQSEQGGYVPLA
                     DPSSPPEKFLRAIDIETGKIAWEIPQVGPPEANYSGVLSTAGGLVFYGETGGGFAAVE
                     AKSGKTLWHFETGQQWRASPMTYMAGGRQYVAVASGSNVLAFALPGR"
     gene            3575..4819
                     /locus_tag="VFA54_05355"
     CDS             3575..4819
                     /locus_tag="VFA54_05355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZS50265.1"
                     /translation="MHFPFAGDQVMGKVDIEYRSRLTRRSALRKLAAFLAGSPLLRGQ
                     QDPFRDQSRVPAMNELLTALDFEPVAYAKIPRDAYDYMALGAEGEVTLRRNRQAFDWV
                     SLVPKGAADSSSVNAAAEVLGIRMAFPIFVSPTATQGQLHPQGELAMHQGATAASGTL
                     MMISVNASFPIEKIAAAAAGPVWYQLYARATVDENRELLERAQQAGCRAVAITADEPV
                     GSYREPTLHDRHMSEGPGSGRGRAAATRNRQVQAPQNAKYGVTGGQPWLDWKLMDAIR
                     SFIKVPLLAKGILTAESARACVAHGLDGIIVSNHGGRALDYSPSTLEALPEVVDAVQG
                     RIPVLMDGGVRRGSDVLKALALGAKAVGLGRAPRWGLAGYGPAGTQRVLEIVQGELVL
                     AMAATGRPRLESIDRTLVKIDF"
     gene            4970..5773
                     /locus_tag="VFA54_05360"
     CDS             4970..5773
                     /locus_tag="VFA54_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS50266.1"
                     /translation="MTKFFAAVLAAVLAYPAAAQLLPPSEAGITMGHVHLNVRDVEAH
                     KKFWVEQIGAKPVTLGRIEGVTVPGLILLFRKQDPTGPVTGEVINHMGLKVKKLSDLL
                     ARFQSAGFRTEKVRIGRENTPQTYVIGPEEFRMELVEDPSLAFPVVSHHLHYFLDEPK
                     AVKQWYVEKLLLKPTMRGPYESADVPGMNLTFAPSRSGPTVPTQGRALDHIGFEVKNL
                     EAYCNRLEQHGVKLDSPYRKLPEIGLAVAFLTDPWGTRIELTEGLNQAR"
     gene            5884..6327
                     /locus_tag="VFA54_05365"
     CDS             5884..6327
                     /locus_tag="VFA54_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS50267.1"
                     /translation="MRRGVLVVLIAAACSPVLAQSPAAPPKRKNSGVVATTIMDRPEV
                     RILRVEIEPRATRATHAHNDVRYHLFIPLTGAVELSIASQEPVDASPGQVYFLNAGTQ
                     HGFRNTGASPARVIEVFVRETAARAGATADENWPAALAAALATRR"
     gene            complement(6397..7791)
                     /locus_tag="VFA54_05370"
     CDS             complement(6397..7791)
                     /locus_tag="VFA54_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50268.1"
                     /translation="MRVWITLTALALLTPLAAECQQNYRVDTPHPRLWLNARRLRLLK
                     RERQRSSERWQELQSLIESGQTLPEEPLVLALQYAVEDKQEAGGRAVSWAVKRTGESS
                     VADPAELRALAVVLDWCYPLFSEADRAKILTRISRAARSLLAQSGMQPFASLTLAAIA
                     SAGDWPDAERTLAALQKKWKVEIWPNAGAGALDAPAARVAFLEACHALRDNLGVDLWD
                     EAPEYFKQFPLYLMLQCYPAPFIVEGVRLHRSAEMDAENSDPALEGRRTRVGELLAVA
                     YDGNSVQAQFLQGWVTHDSYRLRDPWGALYEFLWMNPYQVGLSYYSAPLQLYDAENGR
                     LWARSSWDDDAAWAGYSGGKLQLFADGHRSIVQQSPKPSPVLFPGFAVAAVSGDAAFE
                     ARLIEGEDVFAVGLQAGRTYWVKTGEQKRFVPQVAAKGGILPLHAEPGADATFEIRSS
                     DPNPPAPPKRDSRR"
     gene            complement(8384..8704)
                     /locus_tag="VFA54_05375"
     CDS             complement(8384..8704)
                     /locus_tag="VFA54_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50269.1"
                     /translation="MAVEAEAQSPQQAAEQGAQPTKNLLFTLSDGTKVKRRIPRQRAC
                     NEPGPDTKKGNICAGHLKRWFGFGDEIKQKMGPNPEIYRCEHCHTLYISEDQDQPRTL
                     ILRY"
     gene            9108..9485
                     /locus_tag="VFA54_05380"
     CDS             9108..9485
                     /locus_tag="VFA54_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZS50270.1"
                     /translation="MAKTISKQMTKAQIGHYKKILEEKAIELRKNMLTPAAANIVARR
                     EEPVDVADLSTQSHEEWIFLNRNSLDISLLRQIQEALTRIEDQTYGICQDCGTPISFK
                     RLEAVPWATYCVQCQDRHATSNN"
     gene            9883..13134
                     /locus_tag="VFA54_05385"
     CDS             9883..13134
                     /locus_tag="VFA54_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS50271.1"
                     /translation="MKKLALLLVLFGPIGLRAQQTATSQISGVVTDPSGSAVPNAEIK
                     VTQTETDFTRTLISGPDGAYTIPNLPVGPYRLQVTASGFSVYVQSGIILQVNSNPVIN
                     PALRVGAVSEQVQVSAEVAMTETHENSISQVIDQRRIEDLPLNGRNPTQLVLLSGGAA
                     NGSFTGSSLNGSKNYSTQSVTFSVAGGQLDAVNYVMDGGDNNDAFSNVNLPFPFPDAL
                     QEFSVQTNALPARYGLHSGAVVNLVTKSGSNAIHGDLFEFLRNGDLNARNFFAPVHDA
                     LKRNQFGGTAGGPILRDRLFFFGGYQGTRTRTDPPTTQGIVPNLAMLSGDFTTALSTT
                     CRSAPLTLTGFANDHIPVSQFNQQALNLLTYIPLSNDPCGRITYGIPNNSAEDQYIGR
                     IDFNQSERHSIFSRYFISDYRNPAVFDGKNALSTTKPGVAPRSQSFTVGDTYSLTPTT
                     ISAFHATFNRLRIFRGPADNLISPNTVGINISQYVPNFIYLQVNNFFTLGCGTCSPGF
                     FNTNTLPQIAEDVDLIRGAHQISFGVNYIHVQLNSLSNNIANGQYTFNSSITGYGLAD
                     FLLGQPSAFTQSNPQRQNNRYNYLGLYVQDAWRVSRRLSINAGLRWEPYFPAYDRYGR
                     GSHFELSDFLAGKRSSVFVNGPAGESFYGDPSFPRGDANSHWPNFGPRLGVVIDPAGD
                     GRQTIRASYGILYNTPETYYNIRFVSAPPYGNQINIPNPAGGLTNPYQGFQGGNPFPQ
                     VFPPPKDVAFPPFGVYVNIPLNVKPTYVQLWNVSYQRQLTGNWLVSANYLGSKTTHLW
                     LATEANPAAYLGPSSTTGNTNQRRVLYLIDPAKGGLYGTMGQTDDGANSHYNGLLVSL
                     QHRFSHNFTSLTNYTYSHCIGDGDFTGELTGPLYQNPNNRAGDRGNCGFDRRQIFNHS
                     LVVSSPRFPNKLAQRLAGNWQLSGILSVSTGEFFNVLTGTDRSLTGVNLDRPNVVGNY
                     QLSNASFNHWFNPGAFQANALGSFGNAGHNIMLGPKTVNFDMGLVREFGVREAMRLEF
                     RGEAFNVINHANMDMTIPSSGTNLHNQVSDPLFGSITGAADPRILQLALKLKF"
     gene            13445..>13814
                     /locus_tag="VFA54_05390"
     CDS             13445..>13814
                     /locus_tag="VFA54_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS50272.1"
                     /translation="MWASKRAVSDVLRFAVLLGAAAFWVQFGFAQFTSSVEGTVTDAS
                     QAAVPEAQVTLTNQLTQVVHRTTTSEAGFFRILQLPPGAYRLEVRREGFRTWVENDLI
                     LEGSQARTVYPVLAVGEQAAR"
BASE COUNT         2706 a         4186 c         4247 g         2675 t
ORIGIN      
        1 cgatgcgcgt ggacttgatg gctgcgttca ggcggccgaa gccttcgtaa tcgtgcggcg
       61 gcaggcactc ttccatccaa tagacgcggt acggagcaac catttcggcc atctcgatcg
      121 tgtagcgctc gttccaggac atccagcagt cgagcatgat gtcgccgtcc gggcccaggg
      181 ccttgcgcgt gctttgcacc agctccacat tttttttcat gccctcccgg ccgtcggcgg
      241 gtccgaaggg aatggccagc ttgagcctct tgtatccgaa ctgtatgtgc tgctcgatat
      301 cgttgcccgt gcagtaggcg gggatacggg acttggtttc tccgccgagc agcttgtaaa
      361 cgggcatgcc gagagcgttg ccgatgaggt cccacaacgc cagatcgacg ccgctgattc
      421 catggatcac caggccggcc cggccgtacg acatgctgga gcgccacaaa atatcccaaa
      481 tgcgttccac attgaacgga tcttctccca gcagcagctt ggtgaagtgc tgctcgacga
      541 cataaccgcc gccgggaccg ccgctgccat acccctttac gcccttgtcc gtggagattt
      601 cgaccgtgaa actcgggacc ttatccggat cgggcaggaa cagcgaccgc ttcgccttgt
      661 actccgggta gatcgacatc gggctggcca cttccacgcc gttggtggac cacgcgccgg
      721 gggccggcgt gtagggcggg aggggccgct tgggccgcgt ctttaccaga cgcactcccg
      781 taattttgag tttgcttttt tcctgagcga aggcgagcgg cggcaaccgg atcaggctga
      841 gcgccgacag tccgcccaat cttagaagcg acctgcgatc cataaggcct ccttccgtga
      901 acaatctgcg agagatcagg ctttcccggg gctcacctcc cgggcaaggc gaaagcgagc
      961 acattgcttc cggaggcgac ggcgacatac tggcgtccgc cggccatata ggtcatgggc
     1021 gaagcccgcc attgctgccc ggtctcgaag tgccacagag tttttcccga ttttgcctcc
     1081 acggcggcaa agccgcctcc tgtctcacca tagaagacga ggccgccggc tgtcgagagg
     1141 acgcccgaat agttggcttc cggcggccct acttgaggaa tctcccaggc gattttgcct
     1201 gtttcgatgt cgatggcgcg aaggaacttt tccggggggc tggaaggatc cgctaagggc
     1261 acgtatccgc cctgttccga ctggcggtag atgttgcaat cctcgatggc catcacgtaa
     1321 aaaagcttcg tttccgggtt aaaagtcgtc gcataccaat tcgtcgcgcc gcgcacggcc
     1381 ggacaagttt tggttcccgc cggagtgggc ttgttaccct ccaccacgtg gggccggccg
     1441 ccggccccga tgccgctcgc ccaggtcagc ctgcggacga acggcttccc gagcaggaat
     1501 tcgccggtgg tccggtccag gacgtaaaag aagccgctgc gatttgcttg cagcagcagc
     1561 ttgcgctcgc gtccatgaaa catggcgttc accagaacga gcggctcggt ggcgtcccag
     1621 tcgtgcaggt cgtggggcgt gaattggtaa taccatcgta atttgccggt tttagcttcg
     1681 agcgccaaca cgcaattggt atagagatta tcgccggccc gctggtctcc gtccgtgtcc
     1741 gggaacgggt tgccggtagg ccaaaacagc aggccggtct ccgggtcgta agagccggtg
     1801 agccacgtcg ctccgccgcc ggtttcgagc gctgagccgc gccaggtttc ggatcccggc
     1861 tggccgcgtt gaggcacggt ccagaagcgc catacctgcc ggcccgtccc ggcgttgtag
     1921 gctgcgagaa acccgcgcag cgggccatcg ccgccggaga cgcccgagat caccaggtct
     1981 cccacgacca gcggggcggc cgtcgcgcca tagcggccgg ggccttcagg cattaccacg
     2041 tcccagatca gcgctccggt cagccggtgc aggcaaatca agtgggcgtt atcggtcgcg
     2101 aaatagaccc agtcgcctag aacggctacg ccgcggttgg cgcctttggc ggcgtctccg
     2161 gcgatggtat ccgcggcgct acgggggcgc gaatagcacc agatttcgcg gccagtgcgg
     2221 gaatccagtg cgcagatccg gttgggcgcc gagacataca tgacgccgcc gctcaccacc
     2281 ggggtcatct ccagtcctat gtaagggagc gtgtaagtcc actgaagctg taaccgattc
     2341 gcgttttcag tagtgatttg acgcagggcg ctatgccgat tgcctctgat gtcgccgttg
     2401 taagtgggcc aatcgccggg cgggggccgg agaacgcttt ccatcgcccc ggcgccggcc
     2461 ggttcgaact cgccagtgag agggccggtc gggacgccgc cgagccgggc caggtacgcg
     2521 atgagattgc ggcgctcttc cgcggtggag tggagcgccg gcatcacggt tcctttttcg
     2581 cgggaaacct cgtcgtattc cgtatcgagc aggagatgca tccagccgtc gaacgtttgc
     2641 aattgcaaat catgcttgcc ttgattcctt gcaaaaccgc gcagcacgga accattgcgc
     2701 aggcgaacgc gcgccacgct ccaggtatct tgggggcacc aggcccacgg gggacacgat
     2761 cctgaggagc ggctcccggc tctggccgac ggatcatcca gcgcctgctc taactcagcc
     2821 agagtgagtt ggcgcccgat gtcggaaagg tcggggccgt tggtccggcc tcggccgcgc
     2881 accatgtgac aggaagcgca ctggcctttg ccaaaaaaga atcgctcgcc ggcggccggg
     2941 tctcccggaa gcctgagatc gtaaacggag gcattcaggg aatggaccca cgcggcgagc
     3001 gcctcaagct cgttctccgg gaggcgaaac ggcggcatgc cgggcggggc tccgttccgg
     3061 atgaggtcct gaatctgctt ctcggaccgc ctgcgcagag aacgattatc cgccagcgcc
     3121 ggcccgcgct ctgtgccggc ggccttgtct ccgtggcagc ccgagcagag ttgccgaaat
     3181 tgcgccggcg cgtcccgggc gggagccgga gaggccgcgg gttgagcggc cggattcggg
     3241 aggcaaacgg ccagcgccgc ggaaatgcca gcgaggagag tccaagtgtt ctgcatgaac
     3301 actcagatta acatccaaag aatacgaatc gtcacttcgg gacctgcgcc cgccgtgatc
     3361 acaggagcag gagtcgaggc ggcaaaagcg agcgggagcg attgctctac cgaaacacgg
     3421 gcgggagcag tcccgtgacg caccgtagag aaggaaccgc gtccgcaact ggtcttctgg
     3481 cgcggtggtg gtccgggcac ggcaaccgag gggcatttta cgggcaggtc aaaacctgcg
     3541 cgtgcgattg tggcaaggtg tatactacac gccggtgcat ttccctttcg ccggggatca
     3601 ggttatggga aaggtcgaca ttgaatatcg atcccgtctg acacggcgct cggccctccg
     3661 gaagctggcg gcttttttgg cgggctcgcc gctgctgcgc gggcagcagg atccgtttcg
     3721 agaccaaagc cgcgtcccgg ccatgaacga gttgctcacc gcgctcgatt ttgagccggt
     3781 ggcctacgca aaaattcccc gcgatgcata cgactacatg gcgctgggag ccgaagggga
     3841 ggtcactcta cgccgcaacc ggcaggcgtt cgattgggtg agtcttgtgc cgaaaggcgc
     3901 ggcggattcc agttctgtca atgcagcggc ggaagtgctt ggcatccgaa tggcattccc
     3961 catttttgtg tcgcccacgg cgacgcaagg ccagctacac ccacaggggg aactggctat
     4021 gcaccagggg gcgacggccg cttccggcac gctgatgatg atcagcgtca atgccagctt
     4081 tcccattgag aagatagcgg cggcggcggc cgggcctgtt tggtatcagc tctatgcaag
     4141 agcgacggtg gatgagaatc gcgagctgct cgagcgcgct cagcaggccg gctgccgcgc
     4201 cgtcgccatc acggcggatg agcccgtggg atcctaccgg gagccgacac tgcacgaccg
     4261 gcacatgtcg gaagggccgg gatctggcag gggccgggcc gccgccacgc gcaaccggca
     4321 ggtgcaggcg ccccagaacg cgaaatacgg cgtcaccggc gggcagccct ggctcgattg
     4381 gaaattgatg gatgcgatcc gttccttcat caaggttccg ctgctggcta aaggaatcct
     4441 cacggcggag tccgcgcggg cctgtgtggc tcacggcctg gatggaatca tcgtgtcgaa
     4501 tcatggcggc cgcgccctgg actactcgcc gtctaccctg gaagcgctcc ccgaagtcgt
     4561 ggatgccgtg cagggccgga ttccggttct gatggatgga ggagtccggc gcgggtccga
     4621 tgtgctgaag gctctcgccc tgggggcgaa agcggttggt ttgggccggg caccgcggtg
     4681 gggcctggcc ggttatgggc cggcagggac gcaacgagtg ctcgagattg tccagggtga
     4741 actggtgctg gcgatggccg ctaccggaag gcccagactc gagtccatcg accgcaccct
     4801 ggtaaagatc gacttctgag aaagatcggc ggggaccaag gaccggatga aaccggctga
     4861 aacgccggcg cccttgcggg cccgcctttg cgccgccgac aatggcttcc ggcgccagcg
     4921 agggacttcg tttcgcgccg tgtacactac gcgcccaggg agaattctca tgacaaagtt
     4981 ctttgccgcc gtgctggccg cagttctggc gtacccagcc gcagcgcaac tgctgccgcc
     5041 gagcgaggcg ggcatcacga tgggccacgt gcatctgaat gttcgggatg tcgaggcgca
     5101 caagaagttc tgggtggagc agatcggcgc gaaaccggtt acgctgggaa gaatcgaggg
     5161 agtgacggtg cccgggctga tccttctatt ccggaaacag gatccaaccg gtcccgttac
     5221 cggcgaagtg atcaatcaca tgggcctgaa ggtaaagaag ctctccgacc tgctggcccg
     5281 cttccagtcg gcgggattca ggacggaaaa ggtccgaatc gggcgtgaga acacgccgca
     5341 gacctacgtc atcggcccgg aagagttccg catggaactg gtggaagacc cctcgctggc
     5401 ctttcccgtt gtcagccacc atctgcacta ctttctcgat gagcccaagg ccgtcaaaca
     5461 gtggtatgtg gagaagctcc tgctgaagcc cactatgcgc gggccttacg agtcagctga
     5521 cgttccgggc atgaatctga cattcgctcc cagccgcagc ggtccaacgg tccccaccca
     5581 aggccgggcg ctcgaccata tcggatttga ggtaaagaat ctggaggctt actgcaatag
     5641 gctcgagcaa cacggagtga agctcgattc cccgtaccga aagctgccgg aaatcggctt
     5701 ggccgtcgcg ttcctgacgg acccatgggg aacgcgtatc gaactgacag aagggctgaa
     5761 tcaggcccgg tagccgggcg gaatagccgc tgcgcgcttg cgggcgccgg ctgccgatcc
     5821 ggcttcgagc gggagcggcg ccggccgagc cgggccggat ccagccggct ggaggtgtac
     5881 cccatgcgca gaggcgtctt agtcgttttg attgcggctg cctgctcgcc ggtcttggct
     5941 caaagtccgg ccgctccccc aaaacggaaa aattccggcg ttgtagccac aacaatcatg
     6001 gaccggcccg aggtaagaat cttgcgagtc gagatcgagc cccgcgctac acgcgccacg
     6061 cacgctcaca atgacgttcg ctatcacctt ttcatacccc tcaccggcgc tgtagagctt
     6121 tccatcgctt cgcaagagcc tgtggatgcg tctccgggac aggtgtattt cctgaacgct
     6181 ggcacgcaac acggcttccg gaatacaggg gcctcaccgg caagggtgat tgaggtcttc
     6241 gtcagggaaa ccgccgcccg tgcgggcgca acggcggatg aaaattggcc ggccgcgctg
     6301 gccgccgctt tagccacccg tcgataaagc tccggaacag atctgttgct gccttctatc
     6361 gtttgccggc cggcggagcg ggaagggaag aagcctttat cgtctggagt cccttttggg
     6421 cggcgccggc gggttcgggt ccgacgaccg gatctcgaag gtagcgtcgg cgcccggctc
     6481 tgcatggagg ggaaggattc cgccctttgc cgcgacttgc ggtacgaagc gtttttgctc
     6541 gccggttttg acccaatacg tcctgcccgc ttgcaaccct acggcgaaaa catcttctcc
     6601 ttcgatcagc ctggcctcaa acgcggcgtc gcccgaaaca gccgccacgg caaaaccggg
     6661 aaaaagaacc ggcgacggct tcggagactg ctgcacgatc gacctgtgtc cgtccgcaaa
     6721 gagctgaagt ttgccgccgg agtagcctgc ccaagcggca tcatcgtccc aactgctgcg
     6781 ggcccacagc ctcccgtttt ccgcgtcgta aagctgcaaa ggggcgctgt agtagctcaa
     6841 cccgacttga taggggttca tccacaagaa ctcgtagagc gccccccacg gatcgcggag
     6901 gcgatagctg tcatgggtaa cccatccctg aagaaactgg gcctgcacgc tgttgccatc
     6961 gtaggccacg gccagcagtt cgcccactcg cgttctgcgg ccctcgaggg cgggatctga
     7021 attttccgca tccatctccg cagagcggtg caggcggacg ccttccacga tgaagggcgc
     7081 cggatagcat tggagcatca ggtatagcgg aaattgcttg aaatattcag gggcttcgtc
     7141 ccacagatcg acacccaggt tgtcgcgcaa ggcgtggcag gcttccaaga aagccactcg
     7201 cgcggccggg gcatccagag ctccggcgcc tgcattcggc caaatctcga ccttccattt
     7261 tttctgcaaa gccgccaggg ttcgctctgc atcgggccag tcaccggcgg aggcgatggc
     7321 cgccagggtg aggctggcga acggctgcat gccgctctgt gcaagaagcg atctggccgc
     7381 gcgtgagatg cgtgtgagga tcttggcgcg atccgcctcg ctgaacaggg gatagcacca
     7441 gtcaagaacg accgccaatg cccgcaactc cgccgggtcg gcaaccgaag attcgcccgt
     7501 tcgtttcacg gcccaggaaa ccgcccggcc gccagcctct tgtttatcct ccacggcata
     7561 ttggagggcc aagaccagcg gctcctcagg gagcgtttgg ccgctttcta tcaagctttg
     7621 caactcctgc caccgctcgg acgagcgctg ccgttcccgt ttgaggagcc gcagccggcg
     7681 ggcattcaac cacaaccgcg gatggggggt atcgacccgg taattctgct ggcactcggc
     7741 tgccaggggc gtaaggagcg ccagagcggt cagggttatc caaactctca tgcgaagcgg
     7801 tagcagtcta acacactgtt cgccggccga cgcaggaccg ggaaggaagg atgccgcggg
     7861 cgaaagaagt tttggacaga gtgtcccagg ggtgtgtaca atcgactcag ccaggccggc
     7921 gatcgtacgg aacgggacct ttgtttccat cagttagcgc gcgtgccgtt gtggacggcc
     7981 gtttgccagg caaaaggtga attggtgcta gacaacacct cttcaaacct gccctctgct
     8041 gtattcggca gagtgcgggg ctttcaggtg tgaggacaac gtgcttgcgg gagccggtgt
     8101 ttcgcgattc aagcctgatg cttcgcgctg ctgggtacaa cagagggttt tttacctgcc
     8161 tcggatcgcg gctccgggct ctcgactggc gttcctggtg gaaggtttta ggacgcggac
     8221 ttcaccgggg gcgaagcagt ggcggagtgg tccagttgtg gccggcatcg agatccctcg
     8281 agtctttttc ttggctccgg cctctccagt tcgcggcaaa tgaatccctc ctccggccag
     8341 cgaactggag caggccgctt ctcaaaagag accgcccggc gatctagtag cgcaggatca
     8401 acgtccgggg ctggtcttgg tcctcggaaa tgtagagggt atggcaatgc tcgcagcggt
     8461 agatctcggg gttggggccc attttctgct tgatttcgtc cccgaagcca aaccagcgct
     8521 tgaggtggcc ggcgcagatg ttgccctttt tagtatccgg accgggctcg ttgcaagcgc
     8581 gctgcctcgg aatgcggcgc ttgacctttg tcccgtcaga gagcgtaaat agaagattct
     8641 ttgtgggttg agctccttgt tcagcagcct gctgggggct ctgggcttcc gcttccaccg
     8701 ccatgccgtc ctctcctggc cagaccagaa agtataacat ccggtgggcg caccagtccg
     8761 ggccggttct taaattttgc gaaagggctt gcttttcccc gggccgtccg agtacaatgc
     8821 cccaatcatt ggttggagct ctggccaacc gccgcatatc atcggtacaa attctgcaac
     8881 cgccagcgac ctttctgcgt cagatgggtt agtccaaagc gggcttaagt gcgggctgaa
     8941 gaatttggct cttgctgtac ggaactaaga agcccgcaga gaagggcggg atagcggcct
     9001 ggagggaagg aaaattgcag cggaaactag gctgcacggc tgggatggac gataagtagt
     9061 tagcttttcc ggccccggtg ttggatggcg gagacgcgga ggcatttatg gcaaagacga
     9121 tcagcaaaca gatgacgaag gcccagatcg ggcactacaa gaagattctc gaagagaaag
     9181 ccatcgagct ccggaagaac atgctgacac ccgccgcggc caacattgtt gctcgccgcg
     9241 aagagccggt tgacgtcgct gacctctcaa cccaaagtca cgaggaatgg attttcctga
     9301 accgcaatag tctggatatc agccttctcc ggcaaatcca ggaagctttg acccggatcg
     9361 aggatcaaac ctacggaatc tgccaggatt gcggcactcc gatttcgttc aagcggctcg
     9421 aggcggtacc ctgggcgacc tattgcgtgc aatgccagga ccgccacgcc acctcgaata
     9481 actgagcctg aatgcggcgg ggagttgcac gccgctgtgt gcgcacctga ccgttgattc
     9541 ctacaagcgg agccgcgaca accggctccg ccgaattttc ccgccaaaaa ttcagacgcc
     9601 ggctcgattt ccgcccacgt tgattcgaga gggcttcctg gccgcaggcg atctttcagt
     9661 gtggcgcctt tcgcttttga ggcgcgagca agagacgttt gaagcgacag gcggggctgt
     9721 gccgggggct gaagcccgta tgtagaggca atgcccggac cggggccgga aggcgctggc
     9781 tttagcgata caaataaagt ttcgggaagc gcgccgccgg cttgacgcag cgaagtactc
     9841 agttgtaggc ttgctggaac gccaagcgta aggagacgcg ccatgaagaa gcttgccttg
     9901 ctgctcgtgc ttttcggtcc gatcggtttg agagcgcaac agaccgccac ctcgcagatc
     9961 tcaggcgtcg tcaccgaccc tagcggttcg gccgtgccca acgccgaaat aaaggttacg
    10021 caaacagaga cggatttcac ccgcacgctg atctcaggtc cggatggcgc ctacaccatc
    10081 cccaatctgc cggtaggtcc gtatcgcctc caggtcaccg cttccggttt cagcgtgtat
    10141 gttcaaagcg gcattatcct gcaagtcaat agcaacccgg tgatcaatcc agcgctgcgg
    10201 gtcggcgccg tcagcgagca ggtacaggtc agcgccgaag tagcgatgac ggagacgcac
    10261 gagaactcga tctcgcaagt gatcgaccag cgccgcatcg aggatcttcc gctgaatggc
    10321 cgcaatccga cccagctcgt gctgctttcg ggcggtgcag ccaacggcag ctttacgggg
    10381 agcagcctca acggcagcaa gaattactcg acgcagtcgg taacgttctc cgtggctggg
    10441 gggcagctcg acgccgtgaa ttatgtaatg gatggcggcg acaataacga cgcgttcagc
    10501 aatgtcaatc tgccgtttcc tttcccggac gcgcttcagg aattcagcgt tcagacaaat
    10561 gccttgccgg cccgctacgg gctgcattcc ggagccgtgg tgaacctggt aaccaagtcc
    10621 ggcagcaacg cgattcacgg cgacctgttt gagtttcttc gaaatggcga tctgaacgcc
    10681 cgcaactttt tcgcacccgt gcacgacgcc ctgaagcgca accagttcgg cggcaccgcc
    10741 ggcggaccga ttctgcgcga caggctgttt ttctttgggg gatatcaggg aacaaggacc
    10801 cgcaccgatc cccccaccac gcaaggcatc gtaccgaacc tggccatgct gagcggcgac
    10861 tttactaccg cgctttccac gacttgccgg agcgccccgc ttactttaac cggatttgct
    10921 aacgaccaca ttccggtgag ccagttcaac cagcaggcat tgaatctgct cacgtacatt
    10981 ccgctttcga acgatccctg cggccggatc acctacggca tacccaacaa tagcgctgag
    11041 gatcagtaca taggccgcat cgatttcaac cagagcgaaa ggcactcgat attctcccgc
    11101 tacttcattt ctgattaccg caatccggct gtattcgacg gcaagaacgc cttgagcacg
    11161 accaagcccg gcgtggcgcc gcgcagccaa tcgtttacgg taggcgacac ctactcgctg
    11221 accccgacca ccatcagcgc gtttcacgcc acgttcaatc ggctgcgcat cttccgcggt
    11281 ccggcggaca atctgatcag ccccaatacg gtgggcatca acatttcgca gtacgtcccc
    11341 aatttcattt accttcaggt caacaacttc ttcacgctcg gctgcggcac gtgctcgccg
    11401 gggtttttca ataccaatac gttgccccag attgcggagg atgtggatct gatacgcggc
    11461 gctcaccaga tctccttcgg ggtgaactac attcatgtgc agctcaattc cttgagcaac
    11521 aacatcgcca acggccagta cacgttcaat tcctccatta ccggctatgg cctggcggac
    11581 tttctgctgg gacaacccag cgcgtttacg caatccaatc cacagaggca gaacaaccgt
    11641 tacaactatc tcggcttgta cgtgcaggac gcctggcggg tgagccggcg tctgagcata
    11701 aacgccggcc tgcgatggga accgtatttt cccgcctacg acaggtacgg ccggggcagc
    11761 catttcgagc tttcggattt tctcgccggc aagcgcagtt cggtgttcgt gaacggtccg
    11821 gccggtgagt ctttttatgg cgatccgagc tttccccggg gagacgccaa cagccattgg
    11881 ccgaactttg gaccgcggct cggggttgtg atcgatccgg ccggcgacgg ccggcagacg
    11941 attcgggctt cttatggaat cctgtacaac acgccggaaa cttactacaa catccggttc
    12001 gtcagcgccc cgccgtatgg caatcagatc aacatcccca acccggctgg cgggcttacg
    12061 aatccttacc aggggttcca gggcggcaat ccgtttccac aggtttttcc tccgccgaag
    12121 gacgttgcat ttcctccgtt cggcgtttac gtaaatattc cgctgaatgt aaaaccaacg
    12181 tatgtgcagc tctggaatgt gagctatcag cggcaactga ccgggaactg gctggtcagc
    12241 gccaattacc tgggcagcaa aaccacgcac ctctggctgg cgacggaagc gaacccagcg
    12301 gcgtatctgg ggccgtcttc gaccaccggc aacaccaacc agcgccgcgt gctctatctg
    12361 atcgatccgg ccaaaggggg actgtacggc acgatgggac aaaccgatga cggcgccaat
    12421 tcgcattaca acgggctgct agtctcgctc cagcaccggt tcagccacaa tttcacgtct
    12481 ctgaccaatt acacgtattc tcattgcatc ggcgacggcg atttcaccgg agaattgacc
    12541 gggccgcttt atcagaatcc caacaatcgc gccggcgacc gcggcaactg cggctttgat
    12601 cgccggcaga tcttcaacca ctccctcgtc gtcagcagcc cccgttttcc gaacaagctc
    12661 gcgcagcggc tggccgggaa ctggcagctt tcaggaattc tcagcgtgtc caccggcgag
    12721 ttcttcaacg ttttaaccgg caccgaccgg tcgcttaccg gagtaaatct cgaccgcccg
    12781 aacgtggtag gcaactacca gttgagcaac gccagcttca accattggtt caatccgggc
    12841 gcgtttcagg caaatgcgct gggttcgttc ggcaatgccg gacacaacat catgcttgga
    12901 cccaaaacgg tgaactttga catgggcctg gttcgcgaat tcggcgtgcg ggaggcgatg
    12961 agactggaat ttcgcggcga agcgttcaac gtgatcaatc acgccaacat ggacatgacg
    13021 attccgtcct cggggaccaa tctgcacaat caagtcagcg atcccttgtt cggctcgatc
    13081 acgggggccg cggatccccg aatcttacaa cttgccctga agctgaaatt ctgaatccag
    13141 gcggcgcatt ttttgctcca gagtgcgact cgatggccgt aagccaaagc gcttgtttta
    13201 aaaatgccca gcaatctcat tgccggcggc cccagatgaa ataaacctag gaaagcggat
    13261 attttcacgc gcgaatcaaa gcagacggat cattaatcaa cgatgctgcg tcaggctggg
    13321 tgagcttcga gcttgacagc cgctcaactg ctgtgcttcc ttattacgag cccggcgctt
    13381 gcgggcggtc aatccaagcg ctggtcgatg gggcaagcag gacggtttcc aactggaggg
    13441 atgcatgtgg gctagtaagc gtgccgtcag cgatgttctg cgtttcgccg tgctgcttgg
    13501 tgcggctgcc ttttgggtgc agttcggatt cgcgcagttt accagcagcg tggaaggcac
    13561 cgtgaccgat gcttcgcaag cggcggtacc ggaagctcag gtgaccctta ccaaccaact
    13621 gacccaggtt gttcaccgga cgactacgtc ggaagccggg ttcttccgca tcctacagct
    13681 ccctcccggc gcctaccgtc tcgaggtccg gcgcgaaggc ttccggacct gggtggaaaa
    13741 tgatttgatc ctcgagggca gtcaggcgcg cacggtgtat ccggtgcttg ccgtgggtga
    13801 acaggcggcg cgag
//
LOCUS       DASREK010000108         6428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1380467, whole genome shotgun sequence.
ACCESSION   DASREK010000108 DASREK010000000
VERSION     DASREK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380467"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VFA54_05395"
     CDS             complement(<1..1084)
                     /locus_tag="VFA54_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HZS50273.1"
                     /translation="MSDAFHQRIHSALIDQQLQDALASFAGRLMRERRSSVGGEEFPE
                     YQELRAQANLIKRHTMENLDHYLEILSRKVEEHGGQVAWCRDAGEAQAFIGQLARERG
                     VRLAVKSKSMTSEEIGLNEALEHYGVEATETDLGEFIVQLAHEHPFHIVAPALHKNRR
                     QVAALLSEKLGVAWEEEIPKQTAIARSVLRKKFLEAGMGITGANFLIAESGAVVIVEN
                     EGNARLATSVPRIHVAVAGIEKVIPRAADLAVFLKLLGRSATGQPLTSYTSFLAGPRR
                     PGEMDGPDEFFLLLLDNGRSRILADRDKRQTLYCIRCGACLNVCPVYRKIGGYSYPGT
                     YSGPIGAILTPQLSGITYEPELPFASS"
     gene            complement(1089..1814)
                     /locus_tag="VFA54_05400"
     CDS             complement(1089..1814)
                     /locus_tag="VFA54_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZS50274.1"
                     /translation="MPARVSLFVTCLVDQFFPQIGLAMTRVLERLGYEVDFPEAQTCC
                     GQPAFNSGFWGEARLVGRHFLKVFKGCDYIVCPSGSCTSMVRRHYAEIFRKDAESLEA
                     ANALAPKIFEFSEFLVQVAQREDVGARFEGVVTYHDSCHALRELGLREGPRRLLRSVR
                     GLELREMDAAEECCGFGGTFSVKFDDVSAAMLAGKIDSIRRTGAQSVVSADASCLMQI
                     RGGLLRAGLPIQTLHLAEVLACV"
     gene            1872..3461
                     /locus_tag="VFA54_05405"
     CDS             1872..3461
                     /locus_tag="VFA54_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZS50275.1"
                     /translation="MYDAIIIGAGHNGLAAAGYLARGGWRVLVLEARELVGGACVTEQ
                     LWPGFRVSTAAYLCSLLQPRIVKDLELERFGYQVDAKDPAFFSPFPDGRCLLMWQNRQ
                     KTLEEIGRFSRRDAEVYPKYEEHLERLARFLEPLLLTAPPNLPPRGGGDVIDYLRLAG
                     RLRKLGSADIAALAKIFTQSAAEFLDEWFESDELKVTLATDGVIGANGGPMSPGTAYV
                     LLHHCMGGVGGVRGLWGFVRGGMGAISEAMAASARSRGAEIRCSSKVIRILVRDGRAA
                     GVVLAGGEEIPARAVVSCADPRRTFLGLLEPGHLPDDFVRAIRFYKCEGTSLKINLAL
                     DGLPEFRAQPGASPGPHHRATIHICPGMQYLERAWDDAKYGRPSERPLIEMTIPTLYD
                     PSLAPSGKHIMGVFLQYAPYTLAAGSWDQIRESYADRVLDLISEYAPNIKSIVLHRQV
                     LTPLDIERTFGLTGGNIFQGAMSLDQMFFFRPVAGWARYRTPVRGLYLCGSGTHPGGG
                     VMGAPGFNAARIIRGDLRRSH"
     gene            3617..5494
                     /locus_tag="VFA54_05410"
     CDS             3617..5494
                     /locus_tag="VFA54_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZS50276.1"
                     /translation="MKGRLSNYTLVLSMLAVLVGVGIIREWWTVAMMALLIFAVTLHS
                     SHLHAAKLSAERRHAREVAELHLRTIEALAVAIDAKDNTTHQHLERVVIYAEAIGKEL
                     NLPEDDMKALRAAALLHDIGKLAVPEYIISKPGKLTEEEFEKMKIHTVVGAEILERVQ
                     FPYPVVPIVRSHHERWDGSGYPAGLRGEQIPIGARILAVVDSFDALSSDRQYRRALSR
                     WDAMEAVKKEAGILYDPKIVDVLEKLYPKLEPLIESIARVDVKSAAQEKIVKGQAPAP
                     KLESERTADTSSALHSIVQAREELQVLYQLTQALGTSLSVDETLRLIAEELRKLIPFD
                     SIAVFLIREGKLLPRFVEGVNASLLARMQIPLGEGLSGWVAANGKYSLNGNPAVEPAY
                     LQDPHQFSRLRCALSVPLDSPKGVIGAVTLYSTEHNAAFSLDHLRILKAVGSKAGTTL
                     HNALIHEAMGRSAETDGLTGLPNARALSTRLEQESARATRQQTPYTLFVVDMNGLKKI
                     NDTFGHLMGNKIIKAVGNALRSSVREYDLVARMGGDEFVLLLPGMDASAAAAKRIELA
                     MAVENLRKDFPNIEVSVSMGSASFPDDSRDSEKLMALADRRMYWHKREYHSTRAAITS
                     A"
     gene            complement(5616..>6428)
                     /locus_tag="VFA54_05415"
     CDS             complement(5616..>6428)
                     /locus_tag="VFA54_05415"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HZS50277.1"
                     /translation="PREVRAKVVVLCASTLESTRLLLNSAPGGLCNSSGVLGHYLMDH
                     IFEGGAEGEFPMLEANPWEGPPRRPNGIYIPRFRNITEKSTNGFIRGYGYQGRSSPVF
                     DYDAPGFGAGYKQAVRKGVWRVNLGLFGECLARKENYVELDKDVVDAWGIPALRISMT
                     WSDNEKKLYDDGRLQAAEMLEAAGAKNIRLTGEYSVPGFGIHEIGTARMGDDPKTSVL
                     NRFNQAHDVKNIFVTDGACFVSSACQNPTLTMMAITVRACEYIVDQSRKNQL"
BASE COUNT         1262 a         1977 c         1920 g         1269 t
ORIGIN      
        1 ggctggacgc gaagggcaac tcgggctcgt aagtaatgcc cgaaagctgg ggcgtgagga
       61 ttgcgccgat tgggcccgaa taggttcccg gatagctgta tccgccgatc ttgcggtaca
      121 cggggcagac gttgaggcaa gcgccgcagc ggatgcagta gagcgtctgg cgcttgtcgc
      181 ggtcggccag aatgcggctg cggccgttgt ccagcaacag caggaaaaat tcgtcggggc
      241 cgtccatttc gcccgggcgc cgcggccctg ccaggaacga ggtatagctg gtgagcggct
      301 ggccggtggc gctgcggccc agcagcttca ggaaaacggc cagatcggcg gcgcgcggaa
      361 tcaccttttc gattcccgcc acggccacat ggatgcgcgg cactgaagtg gccaaccgcg
      421 cgttgccctc gttttccacg atcaccaccg cgccgctctc cgcgatgagg aaattggcgc
      481 cggtgatgcc cattccggct tccagaaact tctttcgcaa tacgctccgt gcgatcgccg
      541 tctgcttcgg aatctcttct tcccaggcta caccgagttt ctcggagagg agcgcagcca
      601 cttggcggcg gttcttatga agagcgggcg ccacgatgtg gaacgggtgt tcgtgcgcta
      661 gttgcacgat aaactcgcct aaatcggtct ccgtggcttc cacgccgtaa tgttccagcg
      721 cctcattgag cccgatctct tccgaggtca tcgacttcga tttgacggcc agccggacgc
      781 cgcgctcgcg ggccagttgc ccgataaaag cttgcgcttc cccagcgtcg cggcaccagg
      841 ccacctgacc tccatgctcc tcgaccttgc ggcttaggat ttccaggtag tgatcgagat
      901 tctccatcgt gtgccgcttg atcagattcg cctgagcgcg cagctcctga tattcaggaa
      961 attcctcccc tcccacgctc gaacgccgct cgcgcatcag ccggcccgca aaagatgcca
     1021 gggcgtcctg aagctgctgg tcgatcaagg cgctgtgaat gcgttgatgg aatgcgtcgg
     1081 acatgagatc agacgcaggc cagcacttca gccaaatgaa gagtctgaat aggaagaccg
     1141 gcccggagga ggccgccgcg tatctgcatc aaacagcttg cgtccgcgga aaccaccgac
     1201 tgcgcacccg tgcggcggat tgaatcgatt ttgccggcca acatagccgc cgagacgtca
     1261 tcgaatttga cggagaacgt gcccccgaag ccgcagcact cctccgctgc atccatttcg
     1321 cgcagctcca gcccgcgcac ggagcgcaag aggcggcggg gaccttcccg gagcccgagc
     1381 tcgcgcaggg catgacagga atcgtggtag gtgaccacgc cttcaaagcg tgcgcctaca
     1441 tcctctctct gcgccacctg cacgagaaac tcagaaaatt cgaagatttt cggagccaga
     1501 gcgttggcgg cctccagcga ctctgcatcc tttcggaaga tttcggcgta gtgccgcctc
     1561 accatcgacg tgcacgagcc ggatgggcag acgatatagt cgcaaccctt gaacaccttc
     1621 aggaaatgcc ggccgaccag gcgggcttcc ccccaaaagc cgctgttgaa ggcgggctgg
     1681 ccacagcatg tctgcgcttc cggaaaatcg acttcatacc ccaggcgctc cagcacccgc
     1741 gtcatcgcca ggccaatctg tggaaaaaac tgatctacga gacaggtgac aaagagtgag
     1801 acccgcgcgg gcatcgtatg actccgctat tctaacaggt gccgcgctga aaacagcgca
     1861 gcgaacacgc tatgtacgac gcaattatca ttggcgccgg ccataacggg ctggcggctg
     1921 ccgggtacct ggcgcggggc ggctggcgtg tgctggtgtt ggaagcccgg gagctggtcg
     1981 gaggagcgtg cgttaccgaa caactttggc ccggctttcg ggtgtccacg gcggcatact
     2041 tatgcagcct cctccagccg cggatagtca aagacctcga actggagcgc ttcggctacc
     2101 aggtcgatgc caaggatccg gcctttttct cgccgtttcc cgatggccgc tgccttctga
     2161 tgtggcagaa tcgccagaaa acgctggaag agatcggcag gttttcccgg cgcgacgcgg
     2221 aggtttatcc caagtatgaa gagcacctgg agcggctggc ccggtttctc gagcctctgc
     2281 tgcttacggc gccacccaac ctccccccgc gtggaggcgg cgatgtcatc gactacctgc
     2341 gccttgccgg ccgcctgcgc aaactgggga gcgccgatat cgccgcgctg gccaaaatct
     2401 tcacccagag cgcagccgaa tttctggacg aatggttcga atccgatgag ctgaaggtca
     2461 ctctcgccac ggacggcgtg atcggcgcca acgggggacc gatgtccccc ggcactgctt
     2521 atgtattgct ccatcattgc atgggcggcg tgggaggcgt ccgggggctg tggggatttg
     2581 tgcgcggagg catgggggcc atcagcgagg cgatggcggc cagcgcccgg agccgcgggg
     2641 ccgagattcg ctgctcgtcc aaggtgatcc ggatcctggt tcgcgacggc cgcgccgctg
     2701 gggtggtttt ggccggcggc gaggagatcc ccgcccgcgc cgttgtctcc tgcgccgatc
     2761 cccgccggac tttcctcggc ctgttggagc ccggccatct gcctgatgat ttcgtgcgcg
     2821 ccatccggtt ctataaatgc gaaggaacgt cgctcaagat caatctggcc ctcgatggtt
     2881 tacccgagtt ccgcgcccaa cctggcgcaa gccccggccc acaccatcgc gcaaccatac
     2941 acatctgccc cggcatgcaa tatctcgagc gggcctggga cgacgcaaaa tatggccggc
     3001 cttccgagcg gccattaatt gaaatgacaa ttccgactct ttatgatcct tccctcgctc
     3061 cttcgggcaa gcacatcatg ggggtgtttt tgcagtacgc gccctacacg ctggccgcgg
     3121 gttcctggga tcagattcga gaaagctatg ccgatcgcgt tctcgattta atttccgagt
     3181 atgctccgaa tatcaaatcg atcgtgctcc atcgccaggt gcttactcca ctcgatatcg
     3241 agcgcacttt tggcttaacc ggaggaaata tctttcaggg cgcgatgagt ttggaccaaa
     3301 tgttcttctt tcgtccggtg gccggctggg cgcggtaccg cacgccggtt cgcggcctgt
     3361 acttatgcgg ctcgggaacc catccgggag gcggcgtcat gggcgccccc ggcttcaatg
     3421 cggcccggat cattcgcggg gatttgcgac ggtctcactg aacgtattcc caatgcggta
     3481 gtagcactag ggacataaga gacatagggt atttcccgcg ggaaaatcag tagtacttcc
     3541 ttgcccggag ggactagcgt tcgattggca aaaagcagcc gggacccgag aatcaatcct
     3601 ttgggatccg gcagccatga aagggcgcct ctcgaactac actctggttc tctcaatgct
     3661 ggctgttttg gtaggggtcg gcatcatccg ggagtggtgg actgtcgcaa tgatggcgtt
     3721 gctcatcttt gccgtgaccc tgcattcttc gcacctgcat gcggcgaagc tctccgccga
     3781 gcgccgccac gcccgagaag tcgccgagct gcacctgcga accatcgaag cgctcgccgt
     3841 cgccatcgat gccaaagata acaccaccca ccagcacttg gagcgggtag ttatttatgc
     3901 ggaggcaatc ggcaaggaat tgaatttgcc ggaagatgac atgaaggctt tgcgcgcggc
     3961 ggccctgctc cacgatatcg gcaagctggc cgtgccggaa tatatcatct cgaagcccgg
     4021 caaattgacc gaagaagaat tcgagaaaat gaaaatccac accgtggtcg gagcggaaat
     4081 tctggagcgc gtacaatttc cgtatcccgt ggtgccgatc gtgcggtccc atcatgaacg
     4141 ctgggacggc tcgggatatc cagccggctt gaggggcgaa cagattccga tcggcgcccg
     4201 gattctggcg gtcgtggact cgtttgatgc tctttcttca gaccggcagt atcggcgcgc
     4261 cctttcgcgc tgggacgcca tggaggcggt gaagaaggag gccggcatcc tctatgaccc
     4321 caagatcgtc gacgttttgg aaaagctcta tccaaaactg gaaccgctta tcgaatccat
     4381 cgcccgcgtc gatgtgaaat cggcggcgca ggaaaagatt gtgaaagggc aggcccctgc
     4441 gccgaaactg gagtcggaaa gaaccgccga tacctcctcg gccctgcact ccattgtgca
     4501 ggcccgcgaa gaattgcagg tgctttacca gcttacgcag gctctcggga cgagcttgag
     4561 cgtagacgaa actctgcgcc tcatcgccga ggaactgcgc aaattgatac ctttcgattc
     4621 tattgcggtg tttctaattc gggaaggcaa gctcctgccg cgcttcgtcg aaggcgtcaa
     4681 tgcttcgctc ctggcgcgaa tgcagattcc gctaggcgaa ggcctctcgg gatgggtggc
     4741 cgcaaacgga aaatacagtc ttaacggcaa cccggcagtc gagcccgctt acttgcagga
     4801 ccctcaccag ttcagccgcc tgcggtgcgc cctttccgtg cccctcgatt cgcccaaggg
     4861 ggtgatcgga gccgtgactc tctacagcac cgaacataac gccgccttca gcctcgacca
     4921 cctccggatt ttgaaggccg tcggatccaa agcgggaacg acgctgcata acgctctgat
     4981 ccatgaggcg atggggcggt cggcggagac cgacggcctg accggtttgc caaacgcccg
     5041 cgcgctatcc acccgtctgg agcaggaatc cgcccgcgcc acgcggcagc agacgcccta
     5101 cactctcttc gtcgtggaca tgaacggttt gaaaaaaatc aacgatacct tcgggcactt
     5161 gatggggaat aaaatcataa aagcggtcgg caacgccttg cgcagctcgg tgcgggaata
     5221 tgacctggtg gcccgcatgg gcggcgatga atttgtcctt ctcctgcccg gcatggacgc
     5281 aagcgcggcc gccgcgaagc gcatcgagct ggcgatggcc gtcgagaatc tgagaaaaga
     5341 tttccccaac atcgaggttt ccgtcagcat gggctccgct tcttttcccg acgacagccg
     5401 cgactccgag aaactcatgg ccctggccga ccggcgcatg tactggcaca aacgcgagta
     5461 tcacagcacg cgcgccgcga ttacttccgc gtgaaatgtt cttaacctag agcgtccggc
     5521 cgccttcccc gccgcattca aaacccaagc gtgatcccgc ggacaaaaaa cgcgaggttt
     5581 tgccgaaaat gcggagggcg aatggacggc ctgtcctaaa gctggttctt cctgctctga
     5641 tcgacgatat actcgcaggc ccgtaccgta atcgccatca tggtcaaggt gggattctgg
     5701 catgcgctgg atacgaagca tgcgccatcg gtgacaaaga tgtttttaac atcgtgcgcc
     5761 tggttgaagc ggttcagaac actggtcttc gggtcatcgc ccatgcgcgc cgttccgatt
     5821 tcgtggatgc cgaagcccgg gaccgaatat tcaccggtga ggcgaatgtt cttggcgccg
     5881 gcggcttcga gcatttcggc ggcttgcagg cgcccgtcgt cgtaaagctt tttctcgttg
     5941 tcgctccacg tcatgctgat tctgagcgcc ggaatgcccc aggcgtccac cacgtcctta
     6001 tccagctcga cgtagttttc ttttctggcc agacactcgc cgaacagccc gagattgacc
     6061 cgccaaacgc ccttccgcac cgcctgtttg taaccggcgc cgaagcccgg agcgtcgtag
     6121 tcgaagacgg gcgagctgcg gccctgatag ccgtagccgc ggatgaatcc gttggtcgat
     6181 ttctcggtga tgttgcggaa gcgcggaata tagattccgt tgggccgccg gggcggccct
     6241 tcccagggat tggcttcgag catgggaaat tcgccttccg ccccgccctc gaatatgtga
     6301 tccatcaggt aatgccccag cactccgctg gagttgcaca gacctccggg cgccgaattc
     6361 aacagcaggc gcgtcgattc cagagtggag gcgcacaaca ccacgacttt ggcgcgcacc
     6421 tcgcgcgg
//
LOCUS       DASREK010000109        20285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1216457, whole genome shotgun sequence.
ACCESSION   DASREK010000109 DASREK010000000
VERSION     DASREK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216457"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFA54_05420"
     CDS             complement(<1..749)
                     /locus_tag="VFA54_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50278.1"
                     /translation="MPDLRIFLDPCNPGQFYACCGLIELFDREGARVLSHFALDERLP
                     RRAEFYVTSQVELDLTKLISYLKSAEVRKLNGPDKAEAIHWGDLELDWWLDEFRQETS
                     ELKGWAGRQTSLNLVTELRKLLPADATRLFGALAMTTVRFNLDPRSAWLALDLGYSPN
                     EQRTKESATYPAVELLAAAGLQGFRPCGSRREGFRYHLWLTPLPRVVARTASCRPWPG
                     LPVAAYRFEIERRGQQKCFTLATKEDSSHDGS"
     gene            complement(742..3351)
                     /gene="cas3u"
                     /locus_tag="VFA54_05425"
     CDS             complement(742..3351)
                     /gene="cas3u"
                     /locus_tag="VFA54_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HZS50279.1"
                     /translation="MKSDGEELQPSEWFEHFFVQLSGFDPYPWQTVLFCELLEGRNPD
                     ELCLPTGLGKTAVMHVWLLALSWELLHRPRKQAVPRRLVWVVDRRVVVDQATEEAQLL
                     AKRIKDAPEIEGALERLSVAGKKDNVLAVSALRGERAHNRAWSLDPSRPAIVVGTVDM
                     IGSRMLFSGYGDGPWQRPQHAGLLGQDVLLVNDEAHLTPAFAKLLFKISTVQRSQGLK
                     PFLTIRLSATPRESGPRWPDSLTSDLTGSPHFRRIYTAEKLLQFCFVEKREPIAHEIL
                     KIAAGVDQRQGRILIFVREPENAASLARAIRKGTGSDRIRLITGTMRGWERDRIVEDP
                     IFRLFASNQSPPEPCWLVATSAAEVGANISSDLLITDLDTADHLIQRFGRLNRFGETC
                     GQARLVYHEPDGQKEARLLATLEYLTSLPALDSGRDISLRSLFENPPPSKACSEEPPT
                     AQFSDRVLDAWSQTSLCPHPARPPVEAWLHGKQQDLPETEIAWRDDVFWLASRDVSSM
                     DRREALRRHRVLAHERLREPTSRVWKKLQFLPAETRVLLLHPDGAIGSFPIHALVADP
                     ARLRYSLILLPTGCGSIEDGMFQPHAPAEPTPDYDVADRPAGARRARFIAQFDGEGWL
                     IRPLGISPRAAKPKPIWLGSLDQGSVALLARSLGLDLSWQVQIANPEEGEIPPATLLY
                     FRQPRPQKQTQAEVLLDDHLQAVADMARCLAEKLQLPSDLAQTFEWAGRLHDLGKAAP
                     IWQSAMGGDPARPLAKTARPGSPAQLAGFRHELASLTSGEATLDVPLALRDLALHLVA
                     AHHGWGRPHFPDRALDRRNLRASQTAALECARRFARLQEVYGYWGLAYLEAMFKAADA
                     MVSAETFEQPDYA"
     gene            complement(3449..3847)
                     /locus_tag="VFA54_05430"
     CDS             complement(3449..3847)
                     /locus_tag="VFA54_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50280.1"
                     /translation="MPITASAGGPAQEIRLRLGIPVAVDGRFGRLRGQPGPEEAWSVL
                     AVILADASGIERPIPLTNQGGAVYEYSYLAPARTALRVKVSSNEFLLADGSGSPIDER
                     GQVFSAVTPSVTARSPELRADDRDLGLTSG"
     gene            complement(4012..4785)
                     /locus_tag="VFA54_05435"
     CDS             complement(4012..4785)
                     /locus_tag="VFA54_05435"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZS50281.1"
                     /translation="MPGTPLCFAGKEDVTVRLLATFKRLISEGAVAPGGRLPAERELA
                     QKFQVSRSSLRQALKVLEIMGVISQRVGDGTYVNKAAASLLGEPMEFLILLNGISFHE
                     LMEARIIVEPELAARAAARATMDDLAALRREMAAMKGSAKDHNRLSQHDLQFHQAIFQ
                     AAGNRVCTTMFTVIHQSLHRLIELTSHLVPVEHTLHLHQRIYAAIRQRDPAGARKRMA
                     EHLLDAKGLLARAHDEQQSSYLRDRIKELAPTGLRKGKS"
     gene            complement(4969..7200)
                     /locus_tag="VFA54_05440"
     CDS             complement(4969..7200)
                     /locus_tag="VFA54_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HZS50282.1"
                     /translation="MKLLAYVCLLASTAALPLASQQPIYLDPSQPIEKRVDDLIPRLT
                     LEEKASLLSTTAPAIDRLKIPVMNGWNQSLHGIVWTKPTTMFPVNIGMAATWDPALIH
                     DVAAAIADEGRAIYNLWPTVPGKMEPGQQGQLVTVTPSGERLRHNGLVYRSPVINISR
                     DPRWGRIHEAFGEDPYLTSCMTVAYVKGTQGDDTRYLKLAATLKHYAVNNQEKDRQSL
                     SAKVGERMLMEYYLPHFKAGIVEGRAQSIMSSYNAINGVPNAENRLLLTDILRGMWKF
                     EGFVVPDSGAVQLLVRAHKRYATIEEAAAKTILAGSDLDNGAYAVALPKAVEKGFLTE
                     KDIDQSLRRVLKVRFRLGELDPPEMVPYSKIPPSVIDSPQHRDLALRTARESIVLLTN
                     KNAFLPLDRSKIRSIAVIGPHANFAMTGIGYTGLASKFVKPLDGIKNKVAPGTEVLYA
                     RGSGFLESDNEEASFAEAVSTAKKADVAIAYVGTNQLLEGEGRDRMYINLPLIQQQLV
                     RRVYEANPRTVVVLVNGGPVSLASSGPGIQGRALNVPAVVEMFMAGEEGSNAVAEVLF
                     GDYNPGGKLPYTVYQSVADLPPMDEYDITKGFTYMYFDGKPAYPFGHGLSYTTFNYTN
                     LHVSSQRIPGNGAVTVRVDVQNVGRRAGDEVVQLYVHDLQATLKRPKKELRGFERISL
                     KPGEKKTVSFLLPAEKLAFYDESRKAFVVEPGAFDVLVGSSSEDIRAKGRFEVASPAQ
                     WTN"
     gene            complement(7309..10653)
                     /locus_tag="VFA54_05445"
     CDS             complement(7309..10653)
                     /locus_tag="VFA54_05445"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS50283.1"
                     /translation="MRTNAWRNGDLLLGLAAMMVWWGGSVAAFAQVQAGRIVGAVYDP
                     QRAAVPNASVTVTDVATNISKRVATNATGDYVVTPLNPGSYKITAAAPGFQTTVQTGI
                     ELVVGQAIRVDLELRLGATSTEVQVSAETPLLSTESGTLGQVITNTQIVDLPLNGRSF
                     SELARLAPGDALLPPTGNVQQVRPELVNGNVISGVRGSQTTFLLDGVDVTEQHQGGTW
                     IQTSIDALQEFNVQQNAYSAEFARAGGSFNATTKSGSNSFHGDLFEFLRNDKLDARNF
                     FSPARGILKRNQFGGTFGGPVLIPKVYNGKDKTFFFISYEGQRQRIGQVFNSTVPSEA
                     ERNGDFSASGLNKIYDPLTTGPNPAGNGNVRAQFANNKIPPARLSPQALYFNKYIPLP
                     NTSTGTAVFVPSSAFDQNQVTLRLDREVNASNKLFIRLSIHHNQEDDPAAFPALGYTH
                     LEGPARNVAAALTSNLRANMIHEARFSYMYGEYRSNAYFQGLGAQFDKEAGITGLEGV
                     QDASISSLPAFSFSGYTGFSGNAGDGRPKWQDRWVYEFTDNLTWIRGRHIIKLGTRIH
                     YFEPLFTDSRTHNGSFSYTGIMTENPAAAASTGDAFADWMLGYPASVGRSNPATWWGG
                     YGTYWHFFVQDDLKVGDKLTWNLGLRYEYTPWLNGYRGQVATFDPTQAKSIIVASNTD
                     QIDLGAQPAAAVGYALYKDLIQTSSQAGLPYSITYPDTHQFAPRFGLAWRPFGEDTVF
                     RGGYGIFYESEGTSGRLNFNFLPFSLSETVNADRDVTPTRTTANFFLGAPFGSAVTAA
                     SWTPSPTRMRMGYDQHWNFGVQRQLRKKLLLEVDYVGNKGSFLSGTDAINFPLAGAGS
                     IQNRRPYPRFGNMSYSSQDVSSTYHSLQAKLERRLSAGAWFLASYTFSKSITSANTPA
                     VGGNYAWERAISNFDVPHIFAGSFGYELPFGKGKRFLGSGGLSSWLLGGWQLQSIINF
                     RSGLPYTPTISRDVANTGIGSQRPNRLGSGKLDNPTLAQYFDRSAFAVPASFTYGNSG
                     GDILRGDYSGTVNVSFFKQFAVTENSRLQFRAEVFNLPNAAYFSPPNSSTAAGGSAPV
                     DVASGAKVISTSNDPRQIQFALKYTF"
     gene            complement(11278..12399)
                     /gene="dnaJ"
                     /locus_tag="VFA54_05450"
     CDS             complement(11278..12399)
                     /gene="dnaJ"
                     /locus_tag="VFA54_05450"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZS50284.1"
                     /translation="MARRDYYEVLGVSRDAGEQEIKSAYRKMALKYHPDRNPNNKEAE
                     EKFKEAAEAYSVLSDPQKRATYDQFGHDGLAGAGGAGFNPEAFTDFADIFGDFFGFGD
                     LFGSGGTRRRSQAQRGADLRYDLEISFEDAVFGLHTEIKFPKLETCSRCNGRGAEPGS
                     GPTTCGTCGGRGQVRYQQGFFSISRTCPACHGSGQVITRPCTRCHGEGRTRTERKLKI
                     NIPPGVDNGTRLRLAGEGEPGVNNGPPGDLYVVLKVKEHPIFEREDADLHCTIPVNVA
                     QAALGAEIKIPTLEGEETIKLPPGVQTGGVFRLRGKGVPHVNGHGRGDLVLHINVVIP
                     TKLTREQKKLFEQLLDLLPAENQPAEKGIFERVKDYFAQ"
     gene            complement(12406..12957)
                     /gene="grpE"
                     /locus_tag="VFA54_05455"
     CDS             complement(12406..12957)
                     /gene="grpE"
                     /locus_tag="VFA54_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZS50285.1"
                     /translation="MEELLNQQDEQVSPDSEPSETQAAPEQAGGADIAALTAERDQAI
                     AQKEEIWDRFLRKQAEFENFRKRTAREKEEILEFATMEIIRALLPVLDDFERALKTPS
                     DGEEYRKGIELIHKRLYDILTKSGLTPVESVGKKFDPHLHQAVDTVKDGREDQTIVEE
                     YQRGYEFKGRLLRPAMVKVAVRE"
     gene            complement(13020..14093)
                     /gene="hrcA"
                     /locus_tag="VFA54_05460"
     CDS             complement(13020..14093)
                     /gene="hrcA"
                     /locus_tag="VFA54_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZS50286.1"
                     /translation="MQRPFSLTGRNLEVLNSIIKSYIATGQPVGSRAVSAKRKDRLSP
                     ASIRNVMAELEAEGYLTHPHTSAGRVPTDKAFRHYVRNLTASRLQPSEADFVHVNLSE
                     AATLEERMGRSSHVLAALTHQVGIVVLAPLSRVVLQHVQFVKLAEKRVLMVFVARGDV
                     VRHRIVRLSEEVSQEELDHIAHYVNENFTGWQLGAARREILRRIQEERVEYDAILRRL
                     RLLCLQGFLAADSEARVFLDGASNLVEDAPVLDRDRVRDLLRALEEKEKLIELLDHCI
                     RAEVKIQTPQGAAGETLCVRIGLEDAYPAMKNFALIGAACDMETGLAGRIAVIGPMRM
                     QYERVISAVAHVASVFQNLTENN"
     gene            14458..15033
                     /locus_tag="VFA54_05465"
     CDS             14458..15033
                     /locus_tag="VFA54_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS50287.1"
                     /translation="MHRKTTSRCYGKLGLCAGFVFALLLTAALPLLPQTDKAAPRQLD
                     VPFVPTPQEVVEAMLKLAQVKSTDVVYDLGCGDGRIVITAAKEYGCHAVGIDINPERI
                     QEARENAKKAGVSDLVTFREENLFTADVRPATVVTLYLLPSVNLKLRPRLQSTLKPGT
                     RIVSHSFDMGDWKPDQEQTVDGRSIYLWVIH"
     gene            15350..15616
                     /locus_tag="VFA54_05470"
     CDS             15350..15616
                     /locus_tag="VFA54_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZS50288.1"
                     /translation="MATIILPAAAARPQLNLPEIRRRKGISLEQIADASKINIRYLQA
                     IEEGHFDKLPGGIFNTSYIRQYARAIEYEENALLAYYAACCGGV"
     gene            complement(15834..16754)
                     /locus_tag="VFA54_05475"
     CDS             complement(15834..16754)
                     /locus_tag="VFA54_05475"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZS50289.1"
                     /translation="MDSSVMLCGRTLRNPVLAASGTFGYGIEFEKIVPLDRLGGIIVK
                     GLSREPMAGNPAPRLYETACGMLNSVGLQNIGVRAFVEQKLPALSSLSTPVFANVFGY
                     RVEDYAEVIRILEDAPGLAGYELNVSCPNTSRGGVHFAADSKMLAEVVAAAKRAAQRR
                     PLMVKLSPNVGDICVFARAAEQAGADALSLVNTLIGLAIDARRRRPHLGAGFGGLSGP
                     AIKPIALRMVYQAARAVRIPVVGLGGIATGEDVAEFLLAGAAAVQVGSATFWDPQAPV
                     RIAEEFDQFLREQQIAAARDLIGGLQMENP"
     gene            complement(16759..18339)
                     /locus_tag="VFA54_05480"
     CDS             complement(16759..18339)
                     /locus_tag="VFA54_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZS50290.1"
                     /translation="MLRLLRAALLGVLAAAGSPAAARPRLTVVIVSEQFRPDYFEQFR
                     ASFSAGGFRRLLEGGSVFRNCRYEYAATFPASGAAVLSTGAYPELSGIPAEHWYDRQK
                     HQVVSAVEDASYFTVGSGQPRRPGASPRKLLAGTIADQLRLATAGRSRAVSISLRDQT
                     AVLFGGAKPAGCYWMDEGGRFVTSSYYSDTLPQWAEAFNKTHPALRFRGQPWTAAGAP
                     PSAPPLRVLAPVDPINLQEFFAAFLASPFAVDEEFDFAQEAIAAEKLGEGPSGDLLVL
                     SVSSPFLLGLETGAQSPLVRDLVVRLDRRMEEFFTWLDSRYGVGQVCIAFTATQGLPD
                     SPESLDAGGLHAERVSGEQVAAAVNSRLTALYGRAPRGLSSYVEKYVFPWLYLRQEVT
                     ELYPDAARLAGEAAMSVPGVAGYLVPNGRSSFLTRETRQLLARCWSAGRAGDVLIAYQ
                     PYHFESFGNGRGVAPGSLYNYDTVVPLLLYGPAFQAEIFDAVVNPADFAPTLAAALDI
                     PPPSSANGQVLAEALKER"
     gene            18464..20002
                     /locus_tag="VFA54_05485"
     CDS             18464..20002
                     /locus_tag="VFA54_05485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HZS50291.1"
                     /translation="MGELFDLAIIGGGINGTALAQLAADNGYRIVLLEKTDFGAGVTS
                     RSTRLIHGGLRYLEHGQVKVVRESLRERDALLRDFPHQVRPLPFLVPVYRSDLRKPWW
                     IGLGLEAYSWIARSRLLPRHQHLSPQQAFELEPGLKRENLNAAFLYFDCQAVYPERLA
                     LEMALMAEESGAEVQNHAEVTALLTESKPELRVAGVAANRREYRARLVINAAGPWVDH
                     VLQLMPRGAGRPLLSLIGGAHIVTGSFPGAPSHAIYHEALADRRPFFIIPWRGLWLIG
                     TTETPVSGDPAGVAPSHDEIVYLLRETNLLIPEARLTPDCILYAYAGPRPLLRSESQP
                     AQSITRDHAIYDHEVKDGVAGILTLTGGKLTTARAFAAEALARIAQKLGRPRPVRRPR
                     PKHDWDGIPSRLAEIYGGRAADIVALAEKNPELDRPVCPPKPTTAAEIVHAVTKEKAR
                     TLGDILLRRTGLAFEPDLQEHEIAQVAHVAAPLLGWDNAGIAAALAAYRNEAGRTLYR
                     RPER"
     gene            19999..>20285
                     /locus_tag="VFA54_05490"
     CDS             19999..>20285
                     /locus_tag="VFA54_05490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS50292.1"
                     /translation="MIPAAALAVLVALAWGCLLLRRARLSGLSEFDAIDTQEPPERWP
                     PVSVIIPARNEAAMLRFTLPSALSFYYPQIEVLLVDDCSNDRTAAAAEETAR"
BASE COUNT         4017 a         6258 c         5949 g         4061 t
ORIGIN      
        1 gatccgtcat gggacgagtc ctccttcgtg gcaagagtga aacacttttg ctgcccgcgc
       61 ctctcaattt caaagcggta ggctgcaact ggaagtcccg gccagggacg acaagacgcc
      121 gtgcgggcga ctacacgcgg caggggtgtg agccaaagat gatagcggaa gccttctcgg
      181 cgagaaccgc agggccggaa gccctgcaag ccggctgcgg caagaagttc gacagccgga
      241 taggtcgctg actccttggt ccgctgctcg ttcggggaat aaccgagatc gagtgcgagc
      301 caagctgaac gggggtccag gttgaaacga accgtggtca tcgcaagtgc tccgaataat
      361 ctagttgcgt cggcggggag cagttttcgc agttcagtga ctaggttgag ggatgtctgc
      421 cggccagccc agcctttgag ctcgcttgtc tcctggcgga attcatcgag ccaccagtcc
      481 agctcaaggt cgccccaatg gatcgcttca gccttgtcag gcccgttcag ctttcgcact
      541 tctgcggact ttagataaga aatgagtttc gtcaaatcca gttctacctg actcgtgaca
      601 taaaattccg cccgtcgcgg aaggcgctcg tccaacgcga aatgcgacag tacacgggcc
      661 ccctcccggt cgaacagttc tatcagtcca caacaagcgt aaaactgccc aggattgcaa
      721 gggtctagga aaatgcgaag atcaggcata atctggttgt tcgaaggttt cggccgagac
      781 catggcgtcg gcagccttaa acatcgcttc caggtaggct agtccccagt atccatatac
      841 ttcctggagt cgtgcgaagc ggcgcgcgca ctccaatgcc gcagtctggc tggcacggag
      901 atttctgcgg tccaaagcgc ggtcggggaa atggggccga ccccatccat gatgtgcggc
      961 gaccaagtgc aaggcaagat cgcgcagagc tagcggaaca tccagagtgg cctcgccgct
     1021 tgtaagagaa gcaagctcgt ggcggaagcc cgccaattgc gcgggagagc ccggacgagc
     1081 cgttttggcc aaaggccgcg ccggatcgcc gcccatggca ctctgccaga tcggtgcggc
     1141 tttgccaaga tcatgaaggc ggccagccca ttcgaaggtt tgtgccaaat cagaaggaag
     1201 ctggagtttt tcggccaggc atcgagccat gtcggcgaca gcctggagat ggtcatccag
     1261 gagcacttcg gcttgcgtct gcttttgagg tctgggctgc cgaaagtaga gcagcgtcgc
     1321 cggcggtatt tcgccctctt ccgggttggc gatctggacc tgccaggata aatccagtcc
     1381 gaggctcctc gcgagaagcg caaccgagcc ctgatctaag ctgcccagcc aaatgggttt
     1441 tggcttcgct gcccttggag aaatgccaag cggccggatg agccatccct ccccatcgaa
     1501 ttgcgcgata aaacgggcgc gcctcgcgcc ggcggggcgg tctgccacat cataatcggg
     1561 cgttggctct gcgggcgcat gtggttgaaa catcccgtcc tctatggatc cgcagccggt
     1621 tggcaacaga atgagggagt accgtagccg tgctgggtct gcaaccaaag cgtgaatagg
     1681 aaatgatcca atcgcgccat cggggtgcaa taagaggacg cgtgtttccg ccgggagaaa
     1741 ctggagtttt ttccaaacgc ggcttgtggg ttcgcggagg cgttcatgag caagcacgcg
     1801 gtgccgccgc agggcttccc ggcggtccat agaactcaca tcccgagagg ccagccaaaa
     1861 aacatcatct cgccaggcaa tttctgtttc cggcaagtcc tgctgttttc cgtgcagcca
     1921 agcctccacc ggcggccgcg ctggatgggg gcatagcgag gtttgactcc aggcgtccaa
     1981 tacccggtct gaaaactgtg ccgtgggcgg ttcctcggag caagccttag agggcggcgg
     2041 attctcaaac agtgagcgca gcgaaatgtc tcgtcctgaa tccagcgccg ggaggctagt
     2101 taggtattct agcgttgcta gcaatcttgc ctctttctgg ccgtcgggct cgtggtagac
     2161 cagacgggcc tgcccgcatg tttcaccgaa gcgattcagg cggccaaagc gttggattaa
     2221 gtgatcggcg gtatcaaggt cagtaatcaa caaatcagac gagatgttgg cgcccacctc
     2281 ggccgccgac gtcgccacca accaacacgg ttctggcggc gattggttag acgcaaaaag
     2341 gcggaagatg ggatcttcaa cgatacgatc gcgctcccag cctcgcattg tcccggttat
     2401 aaggcgaatc cgatcggagc cggttccttt gcgaatagcg cgggccaggg aagcagcatt
     2461 ttcaggctcc cgtacaaata ttaggattcg tccctgcctc tggtccacgc ccgccgcaat
     2521 tttaagaatc tcatgagcta tgggttctcg tttctcaaca aaacaaaact ggaggagttt
     2581 ttcagcagtg tagatccgcc gaaaatgtgg gctgcccgtg aggtcggagg taagtgaatc
     2641 cggccagcgc gggccgcttt cccgcggggt ggccgagaga cgtatcgtta ggaatggttt
     2701 gagaccctga gagcgctgaa cggttgaaat cttgaacagc agcttggcaa acgcaggcgt
     2761 aagatgagcc tcatcattta cgaggaggac atcttgtccc aggagccccg cgtgctgggg
     2821 gcgctgccaa gggccgtcac cgtaacccga gaaaagcatc cggctgccta tcatgtccac
     2881 cgtgccgacg acaatggccg gccgggaagg atcgaggctc caggcccggt tatgggcgcg
     2941 ctcgccgcga agtgcgctca ccgccaggac gttatccttt ttaccggcaa ccgaaagccg
     3001 ctcgagtgcc ccctcgattt ccggtgcgtc ctttattcgc ttcgcgagaa gctgcgcctc
     3061 ttccgtagcc tgatccacga cgacgcggcg gtccacaacc cagaccagtc gcctcgggac
     3121 cgcttgcttc cgtggccgat gcagtagctc ccacgaaagc gccagcagcc agacgtgcat
     3181 cacagcggtt tttccgagtc cggtcggcag gcataattcg tcgggattcc ggccctccag
     3241 taattcgcag aacaggacag tttgccaagg atatgggtca aaacccgaca actgcacaaa
     3301 gaaatgttca aaccattcgg atggctgtag ctcctcgccg tcgcttttca tgacacgaaa
     3361 tcagccgccc taacgctaat ggcagagcaa atcttaatcg attttccaaa taatttctac
     3421 gattttctct aaggcgtctg tcgaaatttt atccagaagt aagacctagg tcccgatcgt
     3481 ccgcccgcaa ctctgggctg cgggccgtca cggagggtgt gacagcggaa aaaacttgcc
     3541 cgcgttcgtc gatcggcgaa cccgagccgt ccgccaggag gaattcgttg ctggacacct
     3601 tgactctcaa cgccgtccga gcaggcgcca gatacgaata ttcgtatacg gcgccgcctt
     3661 gattagtcaa cggtattggc cgctcgatgc ccgatgcgtc cgctaaaatc accgcgagca
     3721 ccgaccaagc ctcttcgggt cccggttggc cgcgtaaccg gccgaatcgt ccatctaccg
     3781 cgacgggaat gcccagccgc agccggatct cctgagccgg gccgccggct gaggcagtga
     3841 ttggcactcc tcagtcccac gcgcaagatg accggcggcg ccggctgcga tccgccgcaa
     3901 ttgctcccgc ggccactgtc gcgtccggcc ttataaaatg cagccgctcg aagaaatccc
     3961 caaccagcaa aacgcccgcc gccaagactc aagatatacg ctgccggcgg gtcaggattt
     4021 tccctttcgg agccccgttg gcgccagttc tttaatgcgg tcgcggagat acgagctttg
     4081 ctgttcgtcg tgggcacggg cgagcaagcc cttcgcgtct agcaagtgtt ccgccatccg
     4141 ttttcgcgcc cccgccgggt cccgctggcg aatggccgca tagattcgct ggtgcaaatg
     4201 cagcgtgtgc tcgaccggca caagatgcga ggtgagctca atcaagcggt gcagcgattg
     4261 atggatcacc gtaaacatcg tcgtgcagac acgatttccc gctgcttgaa aaatggcttg
     4321 atgaaattga aggtcgtgct ggctcagccg gttgtggtcc tttgcactcc ccttcatcgc
     4381 ggccatctcg cgccgcagag cagccaggtc gtccatagtc gcccgggcgg ccgcgcgcgc
     4441 cgcaagctcc ggctccacaa tgatgcgcgc ctccatcagt tcgtggaagg agatgccatt
     4501 cagcagaatc agaaactcca tcggctcgcc cagcagagac gccgcggcct tgtttacata
     4561 cgtgccgtct ccgacacgct gggaaataac tcccatgatc tcgagtacct tcagcgcttg
     4621 ccgcaaggag gaccggctga cctgaaattt ctgcgccaac tctctttcgg caggcaggcg
     4681 gccgccggga gcaaccgccc cttcggagat gaggcgcttg aaagtggcca gcaggcgcac
     4741 agtaacatcc tctttgccgg cgaaacacaa aggcgtacct ggcatgagac gcttcaacgc
     4801 ttaccttctg ggatcggggg caggaccccg gcgatttctc ctctgagcgt ctgtgacccg
     4861 gcaggagctt tctcagactg cccagccatc gagatgaacg gcgcagcgcc tgcgcacacg
     4921 gaataaaacg aaatctcggc tcggccgccg gaggtcgttg tcattgtctc agttcgtcca
     4981 ttgcgccggc gaagcgacct cgaaccggcc ctttgcccgg atatcctcgg aggagcttcc
     5041 gaccaagacg tcgaaggcgc ccggttcaac tacgaaggcc tttcggcttt cgtcgtagaa
     5101 ggccagcttt tcggctggca gcaggaaaga gacggttttc ttttctccag gcttcaggct
     5161 gatccgctcg aagccccgca gctctttctt cggccttttg agggtcgcct gcaagtcgtg
     5221 cacataaagt tgcacgactt cgtcgccggc gcgccggccg acgttctgta catccacgcg
     5281 gactgtgacc gcgccgttgc cgggaatccg ttgggaggaa acatggaggt ttgtatagtt
     5341 gaaggtcgta tagctgagcc cgtgaccgaa cggatacgcc ggtttgccgt caaagtacat
     5401 ataagtgaac cctttggtga tgtcgtactc gtccatgggc ggcaaatccg ctactgattg
     5461 atacaccgta taaggcagct tgccgccggg attatagtcg ccgaacaaaa cctcagccac
     5521 cgcgttgctg ccttcctccc ccgccatgaa catttcgacc accgccggaa cgttgagcgc
     5581 ccggccttgg attccgggcc cggagctggc gagcgaaacc gggccgccat tcacgagcac
     5641 caccacggtc ctcggattgg cttcgtaaac tctccttacg agctgttgtt ggatcagagg
     5701 caggttgatg tacatcctgt cgcggccctc cccctccaat agctgattcg tacccacata
     5761 cgctatcgct acgtccgcct tcttcgcagt ggaaacggcc tccgcgaagc tggcttcttc
     5821 gttgtcggac tccagaaatc cgctgccgcg cgcgtaaagc acctccgtgc ccggcgcgac
     5881 tttattcttt atgccatcaa gcggcttgac gaacttggac gccaagccgg tgtagccgat
     5941 tccggtcatg gcgaagttag cgtgcgggcc gatgacggcg atgctcctga ttttgctccg
     6001 gtcaagcgga aggaaggcgt tcttattggt caagagcacg atcgattccc gggcggtccg
     6061 gagcgcgaga tcccgatgct gtggggaatc gatcaccgag ggaggaatct tgctgtaggg
     6121 caccatttcc ggagggtcca actcacccag ccggaacctc actttcagaa cccggcggag
     6181 cgattgatca atgtcctttt cggtcaagaa gcctttctcg acggctttcg gaagcgcaac
     6241 tgcataggcg ccgttgtcga ggtcgctccc cgcgagaatc gtcttagccg ccgcttcctc
     6301 aatcgttgca taccgcttgt gcgctctgac caggagctgc acggctccgc tgtcggggac
     6361 gacaaaaccc tcgaacttcc acatcccgcg caggatgtcg gtgagtagca gcctgttttc
     6421 cgcgttggga acgccattga tggcgttgta cgacgacatg atcgactgcg cccggccctc
     6481 cacaatcccc gctttaaagt gcggcagata gtattccatc agcatccgtt cgccgacctt
     6541 cgccgaaagg ctctggcggt ccttctcctg gttgttcacg gcgtaatgtt tgagcgtagc
     6601 agccagtttc aggtatctgg tgtcgtcgcc ttgtgtgcct tttacatagg caaccgtcat
     6661 gcacgaggtg agataaggat cctcgccgaa agcttcatgg atccggcccc agcgtgggtc
     6721 gcggctgatg ttgatcaccg gcgagcggta gaccaggccg ttatgccgca ggcgctctcc
     6781 gctcggcgta acggtcacta gttggccctg ctggcccggc tccatttttc cgggtacggt
     6841 gggccacagg ttgtagatgg cccggccttc gtcggcgatc gcggcggcga cgtcgtggat
     6901 cagcgccgga tcccatgtgg cggccatccc gatgttcacc gggaacatgg tcgtaggctt
     6961 cgtccaaacg atgccgtgca agctctgatt ccagccgttc atcactggga tctttagccg
     7021 gtcaatcgcg ggcgccgtcg tgctgagcag cgaggccttc tcctcgagcg tcaagcgcgg
     7081 aattagatcg tccacccgct tttcgatagg ctgcgaagga tcaaggtaaa tgggttgctg
     7141 gctcgcaaga ggcagcgcag ccgtcgaagc cagcaggcaa acgtaagcaa gaagtttcat
     7201 cgcaaaccct ctttccagaa atggcgaagc gccgcggcgt gtcgtgttct ccagtctgat
     7261 ccagctttgc cctgccttgc ttatcggcgc cgctccaagc tcggcccttt aaaacgtgta
     7321 tttcaaagca aactgtatct gccgcgggtc attggaagta ctgatgactt tcgcccccga
     7381 ggcgacgtct accggcgcgc ttccgcccgc cgccgtggag ctgttgggcg ggctgaaata
     7441 agcggcgttg gggagattga agacctcggc gcggaattgc agcctggagt tttcggtcac
     7501 cgcaaattgc ttgaagaagg aaacgttgac ggttccgcta taatcgcccc gcagaatgtc
     7561 cccgccggaa ttgccgtaag taaatgaggc cggcacggca aaagcgcttc tgtcaaaata
     7621 ttgagcgagg gtcgggttgt ccagctttcc cgatccgaga cgattcggcc gctggcttcc
     7681 gatccccgta ttcgctacgt cgcgcgaaat ggtcggcgta tagggcaatc cgcttcgaaa
     7741 attaataatg ctctgcaatt gccagccgcc caagagccag ctgctcagcc cgccgctgcc
     7801 gagaaaccgc ttccctttac caaatggcaa ttcatagccg aaactcccgg caaaaatgtg
     7861 cggcacgtca aaattcgaaa tcgccctttc ccaggcgtag ttcccgccta ccgctggagt
     7921 gttcgcgctg gtgatactct tggaaaacgt gtaggaagcc aggaaccagg cgccggcgga
     7981 gagtcgtctt tcgagcttgg cttgcagaga gtggtatgtg ctcgagacat cttgcgagct
     8041 atagctcatg ttgccgaagc gcggataggg ccggcggttt tgaatcgatc ctgcgcctgc
     8101 cagaggaaaa ttgatcgcat cggtgccgct taagaaggat cctttgtttc ccacgtaatc
     8161 cacttccaga agcagtttct tccggagttg gcgctggacg ccgaaattcc agtgctggtc
     8221 atagcccatc cgcatccgtg tggggctggg cgtccaactg gccgcggtca ctgcggaacc
     8281 gaatggcgcg ccgagaaaga aattcgccgt cgtacgtgtg ggagtcacgt cgcggtcggc
     8341 gttgacggtt tccgacaggc tgaagggaag aaaattgaag ttcagccgcc cgctggttcc
     8401 ttctgattcg tagaaaattc catagccgcc ccggaaaacc gtatcttccc cgaaggggcg
     8461 ccaggccagg ccgaatcggg gcgcaaactg gtgcgtatcc ggataggtga tgctgtaagg
     8521 cagcccggcc tggctgctgg tctggatcag gtccttataa agggcatacc cgacagccgc
     8581 cgcaggctgg gcgcccaaat cgatctgatc tgtattgctg gcgacgataa tggatttggc
     8641 ctgcgtcgga tcaaatgtgg cgacttggcc ccgatagccg ttcaaccacg gcgtgtattc
     8701 gtagcgcagc ccgaggttcc aggtcaactt gtcgccgacc ttcaaatcat cctgaacaaa
     8761 aaagtgccag tacgttccat acccgcccca ccacgtcgcc ggattcgacc ggcccacaga
     8821 agccgggtag cccagcatcc agtcggcaaa cgcatcgcct gtgctggcgg cagcggccgg
     8881 gttttcggtc atgatgcccg tatagctgaa gcttccattg tgggtgcgcg aatcggtgaa
     8941 aagcggctca aagtaatgga tgcgtgttcc cagtttgata atatgccttc ccctgatcca
     9001 cgtcaggtta tcagtgaatt catagaccca ccggtcctgc cactttgggc gcccgtcgcc
     9061 ggcgtttccc gaaaaacccg tatagcccga gaagctgaaa gcgggcaggc tggaaatcga
     9121 ggcgtcctga accccttcaa gccccgtaat tcccgcctct ttgtcgaact gcgctcccag
     9181 cccctggaag taggcgttcg accgatattc cccatacata tagctgaacc gggcctcgtg
     9241 aatcatattc gcccgcagat tgctcgttaa agcggccgcg acgtttcgcg ccggaccctc
     9301 cagatgggtg taccccagag ccggaaatgc ggccgggtcg tcctcctgat tgtggtggat
     9361 gcttaaacgg atgaaaagct tgttgctcgc gttgacctca cggtcgaggc gcagcgtcac
     9421 ctgattttgg tcgaacgcgc tggagggtac gaagacggcg gtgcctgtgg acgtattcgg
     9481 cagcggaatg tatttgttaa agtacaacgc ctggggagag agccgcgctg gaggaatctt
     9541 attattagca aattgggcgc gcacgttccc gttgccggcg ggattcggcc cggtggtcag
     9601 agggtcatag atcttgttca aaccgctggc gctgaaatcc ccgttccgct ccgcctcgct
     9661 gggcactgtg ctgttgaaaa cctgtccgat ccgctgcctc tgcccttcat agctgatgaa
     9721 gaaaaaggtt ttatccttcc cgttataaac cttcgggatc aaaaccggtc ctccgaaggt
     9781 gcccccgaac tggttgcgct ttaaaattcc ccgggctggc gaaaaaaagt tgcgggcgtc
     9841 gagcttgtcg ttgcgcagga attcgaacag atctccgtgg aaactattcg agcccgactt
     9901 ggtcgtagca ttgaacgatc ccccggcgcg cgcgaactcg gccgaatacg cattctgctg
     9961 gacgttgaac tcctggagcg cgtcaatcga ggtctgaatc caagtgccgc cctggtgctg
    10021 ttcggttacg tccaccccgt ccagcagaaa agtggtctgc gagccccgca cgccgctgat
    10081 cacgttgccg ttgaccagct ccggacggac ctgctgcacg ttgcctgtag gcggcagcag
    10141 ggcgtctccg ggggcaaggc gcgccagctc gctgaaactc cggccgttga gcggcaggtc
    10201 cacaatttgg gtattggtga tcacctggcc caatgtgccg gactcggtgc tcagcagcgg
    10261 cgtctcggcg cttacctgca cttctgtgct ggtggccccg agccgcagct ccagatccac
    10321 gcggatcgcc tggcccacaa ccagctcgat cccggtctgg accgtagtct ggaatccagg
    10381 ggccgctgcc gtaatcttgt agctccccgg attcagcggc gtgactacgt aatcgccggt
    10441 ggcgttcgtg gctacccgct tggatatgtt ggtggcaacg tcggtaaccg tgacacttgc
    10501 attgggaacg gcggcgcgct ggggatcgta cacggcgccc acgatccgcc ccgcttgcac
    10561 ttgcgcgaag gccgcaacgc tgccgcccca ccaaaccatc attgcagcca ggcccaacag
    10621 caggtccccg tttctccatg cgttggttcg catcgtccgg atcctccctc agccgcccgc
    10681 gtcttaagcg cccctcgcac aggcggcgcg ctttggctga agatggtata gccaatgtcc
    10741 cgcagtgtca agaaaaaagc tccgcgattt cgttggccgc gttcacggcg gtctcgactg
    10801 acaccagtcg ccccaggccc cctgccgatt tccccctcct gccgctctag ccggcgcgaa
    10861 aataattggt tcgaccaaac cgcgcctctg ttcggcgttt ttcgcactcg cctttctcgc
    10921 cgggcgggtg tgcgccccgg aaagcgcact tcggcggttg gacaaccagg gcgcggaagc
    10981 ttggatggcc ttccacagcg cgaccggcag cgactcggct cggacggagg ttagctggcg
    11041 ccgagtccgc agccgcgcgc cgcgccgctg gatgtcccgc gtatagaagg ccacgagtat
    11101 gtattcgcaa tttaggaggt cagactgtaa attctgacac aggaaccgct gaactggagg
    11161 catggcgtgg gcttcctcgc cgccgggaaa tccgctggcg cgggcggcca aacaggaccg
    11221 atccctcact gctgctggat ttctgaattt tttcacctgc cctgcgtagc cgaagcgcta
    11281 ctgggcgaaa taatctttga cccgctcgaa aattcctttt tcggcaggct gattttcggc
    11341 aggcagcaag tcgagcagtt gctcgaagag cttcttttgc tcgcgggtca gcttcgtcgg
    11401 gatgacgacg ttgatgtgca aaaccagatc ccctcggccg tggccattca cgtgaggcac
    11461 ccctttcccg cgcagccgga agacgccgcc ggtctggact ccgggaggca gcttgatcgt
    11521 ctcttcgcct tcgagagtgg ggatcttgat ttcagctccc agggcggcct gagccacgtt
    11581 gaccggaatc gtgcaatgca ggtcggcgtc ttcgcgctcg aagatcggat gctccttgac
    11641 cttgagcacg acatatagat ctcccggagg gccattgttg accccaggct ctccctcgcc
    11701 agccaggcga agccgggtac cgttatcgac gcccggggga atgttgatct tgagctttcg
    11761 ctcggtgcgg gtgcgccctt cgccatggca acgggtgcaa ggccgcgtaa tcacctgtcc
    11821 cgagccgtga caggccgggc aagtacggct gatcgagaaa aacccctgct ggtatcgcac
    11881 ctggccacgg ccgccgcacg taccgcacgt agtcgggccg cttccgggtt ccgctccccg
    11941 gccattgcag cgcgagcagg tctcgagctt tggaaacttg atttcggtgt gtaggccgaa
    12001 taccgcgtcc tcgaagctga tctcgaggtc gtagcgcagg tcggcgccgc gctgcgcctg
    12061 ggatcgccgg cgcgtgccgc cgctgccgaa caagtcccca aatccgaaga agtctccgaa
    12121 aatgtcggcg aaatcggtga acgcttcggg attgaacccc gcgccgccgg ccccggccag
    12181 tccatcatgc ccaaactggt cgtaggttgc ccgcttctgc gggtcgctga gcacactata
    12241 agcctctgcc gcttccttga atttctcttc cgcctctttg ttgttcggat ttcggtctgg
    12301 gtggtacttt agcgccatct tgcgataggc gctcttgatc tcctgctctc cggcgtcgcg
    12361 gcttacaccc agaacctcat agtagtcgcg tctcgccacg gcttgttatt cccgcactgc
    12421 tactttcacc atggccgggc gcaacaagcg gcccttgaac tcatatcccc gctgatactc
    12481 ttccacgatc gtctgatcct ctcgcccatc ctttaccgta tcgaccgcct gatgcagatg
    12541 ggggtcaaat tttttgccaa ccgactcgac cggggtcagc ccgcttttcg tcagaatatc
    12601 gtacaaccgc ttgtgaatca gttcaatccc cttgcgatat tcttcgccgt ccgacggcgt
    12661 cttcagagcc cgctcgaagt cgtccagcac cgggagcaag gcccggatga tctccatcgt
    12721 cgcaaactct agaatttctt ctttttcacg agccgtgcgc ttgcggaagt tctcgaattc
    12781 cgcctgcttg cgcagaaagc ggtcccaaat ctcctccttc tgcgctatgg cttgatccct
    12841 ctcggcggtc agggcggcta tgtcggctcc gcctgcctgc tcgggagcgg cctgcgtttc
    12901 cgacggctcg gaatcgggcg aaacctgctc gtcctgttgg ttcaacagct cttccatacg
    12961 cttatgttat cagaaaaagc cggcgctggc cggcaggagt cggcggactg tgcccgtttt
    13021 cagttgttct cagtaaggtt ctgaaaaacg ctggccacgt gcgctacggc tgatatcaca
    13081 cgttcgtatt gcattcgcat cggtccgatt accgcgatgc gcccggctag gccggtttcc
    13141 atatcgcaag cagcgccgat aagggcgaaa ttcttcatgg ctggataggc gtcttccaag
    13201 ccgattcgga cgcaaagcgt ttctccggca gcgccctgtg gcgtctggat tttcacctca
    13261 gcccgaatgc agtggtcgag cagctcgatc agcttctcct tttcctcgag cgcccgcaac
    13321 aggtcccgaa cccggtcacg gtccagaacc ggagcatctt ccaccaggtt cgatgcgcca
    13381 tccagaaaga cccgcgcctc ggaatcagcg gccaaaaagc cctgcaaaca gagaagccgc
    13441 aagcggcgta aaatcgcgtc atactccaca cgctcttcct ggatgcgccg cagaatttcg
    13501 cgcctggccg cccccagttg ccagccggtg aaattttcgt tgacgtaatg cgcgatatgg
    13561 tcgagctctt cctgcgagac ttcttccgag aggcgcacga tccggtgccg caccacatcg
    13621 ccccgcgcga cgaataccat caatacgcgc ttttccgcca gttttacaaa ctgcacgtgc
    13681 tgaagaacca cccgggaaag cggcgccagt actacgattc ccacctggtg cgtgagtgcg
    13741 gccagcacgt gggaggatcg ccccatccgt tcctccaacg tagccgcttc gctcaaattg
    13801 acgtgaacga aatccgcttc cgagggctga agccggctgg ctgtcaaatt tcttacgtag
    13861 tggcggaagg ctttgtccgt cggcaccctt cccgccgagg tatggggatg tgtaagatac
    13921 ccttccgctt ccagttcagc cataacattg cgaatcgagg ctggactcag ccggtctttg
    13981 cgcttggcgg aaacagcacg ggagccgacc ggctgtcctg tagcaatgta gctcttgata
    14041 atcgaattca gaacttcgag gtttctacct gtaagggaaa acggcctctg catgccacac
    14101 tcccttgggc gctgccgcac gacgcccatg ccgattctaa cctcttgaaa cataaggtgt
    14161 caatacgctt cggccgcgat cgaagcacta gaaagaggtc acggatggac tctgccacgg
    14221 ccggtatacg caggcgcgga aggagaggcc gcccgcggta cactatagcg aagcaggccg
    14281 cccgaatgcg ggtcgcgagc tcggtggaac tcatgtgcgc cgtgcgggcg ccatcagttc
    14341 cgctcagcct ggtgaaaacg cggccgtgtc gcgcctgagg cggtccgcga acgttttcgt
    14401 tttcgcccgc gcgtatgggg acttctcccc tcatgcgccg cagataagga gatgagtatg
    14461 catcgaaaaa cgacttcccg ctgctatggc aaactcggtc tttgcgccgg attcgtcttc
    14521 gcgctgctgc tcacagccgc tcttccgctt cttccccaaa ccgataaagc ggcgccccgg
    14581 caactcgatg ttccttttgt accaaccccc caagaggtcg tcgaggccat gctgaagctg
    14641 gcgcaagtga aatccaccga tgttgtctat gatctgggtt gtggcgacgg acgcatcgta
    14701 ataaccgccg ccaaagaata cggttgccac gccgtcggta tcgatatcaa ccccgagcgg
    14761 atccaggaag cccgcgagaa tgccaaaaag gcgggtgttt ccgaccttgt taccttccgc
    14821 gaggaaaacc tgtttaccgc cgacgtgcgg ccagctacag tggtcacgct ctatctgctt
    14881 cccagcgtga atctaaagct ccggcccagg ctgcaatcca ctctgaaacc cgggacgcga
    14941 attgtctctc atagctttga tatgggagat tggaagcccg atcaagagca gactgtggac
    15001 gggcgttcta tctacctatg ggtcattcat tgagacgcat agacccatag gaaagaggct
    15061 tacagtactg cgcggggacc gtcctcgaga acgcgcttca aaccgcaaac gggcggatcg
    15121 gcagatttat cggctgattc gccctccagc ctaaaattga gtctgccagc cgagctggat
    15181 tttctaatgt tgtttcaggc cggatgctag gcgtttttcc caattatcca cctgcacggt
    15241 tggatttaaa ttaattacaa attgggccga tgagaagaaa ggcattggga tgtgaggccc
    15301 atccggaagc tgaggggctc ctttcctggg agttggactg aggaaagaga tggctacgat
    15361 aattctcccc gccgcagctg cccggcccca gttgaacctg ccggaaatcc ggcgcagaaa
    15421 aggcatttca ctcgagcaaa ttgccgacgc cagcaaaatt aatatccgtt atctccaagc
    15481 tatcgaggaa gggcatttcg acaagctgcc gggcggaatt tttaatacca gctatatccg
    15541 tcaatatgcc cgagccattg aatacgaaga gaacgccctg ctagcctatt acgccgcctg
    15601 ttgcggcggc gtgtagccgg gcgcgcccgt cggccgggcg ctctgcgccg cccaaccgca
    15661 cctttcgata catagacaaa cggaagcggc ccttggaagc tcccagatgt tttctcgatc
    15721 tttgcccgaa aagactgacc ggccctgctg cccacagtct cggcaagtcc aaaatttctt
    15781 tgctgcgagc agagggggcg cggaaagctt cagccgcagc cccggcatcg ccctcatggg
    15841 ttttccattt gcaacccgcc gatcagatcg cgcgcggccg cgatctgctg ctcgcgtaaa
    15901 aattggtcaa actcctcggc gatccggacc ggagcttgcg gatcccagaa tgtcgccgat
    15961 cccacctgaa ctgcggcggc tcccgctagc aaaaactcgg ctacgtcctc gcccgtcgca
    16021 atgccaccca gccccacgac aggaatccgc accgcgcggg cagcttgata gaccatgcgc
    16081 agcgcgatcg gcttgatcgc cggtccagag aggccgccga accctgcgcc gagatgaggc
    16141 cggcggcggc gggcgtcgat cgccagcccg atcaatgtgt tgaccaggga gagcgcatcg
    16201 gctccagcct gctccgccgc ccgcgcaaat acgcagatat cgcccacgtt gggcgacagc
    16261 ttcaccatga gcggcctccg ctgcgcggcg cgcttggcgg cagcgacgac ttcggccagc
    16321 atcttcgaat ctgccgcaaa gtggacgccg ccgcggctgg tgttcgggca ggaaacgttc
    16381 agctcgtatc cggccagacc gggcgcatcc tccaggatcc ggatcacctc agcgtagtct
    16441 tccacccggt atccgaagac attggcgaac accggagtcg ataaagacga cagcgccgga
    16501 agtttttgct ctacgaaggc ccgcaccccg atgttctgta atcccaccga gttgagcatg
    16561 ccgcaggcgg tttcatacag ccgcggcgcc ggatttccag ccatgggctc gcgggacagg
    16621 cctttgacga tgatgccgcc caggcggtcg agcggaacaa tcttttcaaa ttcgattcca
    16681 tagccgaacg tgccgctggc ggcaagcaca ggattgcgca gcgtccggcc gcaaagcatt
    16741 acggagctgt ccaacctgtc acctttcttt caaagcctcc gcgagaacct ggccgttcgc
    16801 agagctgggg ggcggaatat ccagggctgc cgccagcgtc ggcgcgaaat ccgccgggtt
    16861 gaccacagcg tcaaaaattt ccgcttggaa ggcggggccg tacaacaaga gcgggaccac
    16921 agtatcgtag ttatacaaag atccgggagc tacgccccgt ccgtttccga agctttcaaa
    16981 atggtaaggc tggtaggcga tcaaaacatc gccggccctg ccggcagacc agcagcgcgc
    17041 cagaagctgc ctggtttccc gcgtcaggaa gctcgatcgg ccattgggaa ccaggtaccc
    17101 cgccactcct ggaaccgaca tagccgcctc tccagccagg cgggcggcat cgggatacag
    17161 ttcagtcact tcctgtcgca ggtagagcca ggggaaaacg tatttctcga cgtagctgct
    17221 caggccgcgc ggtgcgcgcc cgtaaagcgc cgtcagccgg gaattcaccg ccgccgccac
    17281 ctgctcgccc gatacacgct ccgcatgcaa cccgccggcg tccaggctct ccggactatc
    17341 cggcaggccc tgagtagccg tgaacgcgat gcagacctgg ccgacgccgt agcgggaatc
    17401 gagccaggta aagaactcct ccattctccg gtcgagccgc acaaccaaat cccggacaag
    17461 cggcgattgc gccccggttt cgagacccag caggaaaggc gaagacacgc tcagcaccag
    17521 caggtcgccg gaaggtcctt cgccaagttt ttccgcagcg attgcttcct gggcgaaatc
    17581 gaattcctca tccacagcga aaggagaggc caggaaggca gcaaaaaatt cctgcaagtt
    17641 gatcggatcc acgggagcaa gaacgcgcag cggcggcgcg gaaggaggcg cgccggcagc
    17701 cgtccacggt tgtccccgga agcgcaaggc gggatgcgtc ttgttgaagg cctcggccca
    17761 ttgcggcagc gtatctgaat aataggaact ggtgacaaag cggcccccct cgtccatcca
    17821 gtagcatccc gccggtttgg ccccgccgaa caacaccgcc gtctggtcgc ggagggaaat
    17881 ggagaccgcc cgcgagcggc ctgccgtcgc caggcgcaat tggtccgcga ttgtgcccgc
    17941 cagcagcttc cggggtgaag cgcccgggcg gcgcggctgg ccggaaccga cggtgaaata
    18001 ggacgcgtcc tctaccgcgc tgaccacctg atgcttctgg cggtcatacc aatgctcggc
    18061 gggaatgccg gatagttcgg gataagcgcc ggtcgacaat acggcggcgc cggaagcagg
    18121 aaatgtggcg gcatactcat aacggcagtt acggaagacc gagccgccct ccagcagcct
    18181 gcgaaaacct ccggcagaaa aggaagctcg aaattgttcg aaataatcag gacggaactg
    18241 ctccgagaca atgacaacgg tcagcctcgg ccgagcggca gccggagaac cagccgccgc
    18301 cagcactccc agcaatgcgg cccgcagaag ccgaagcata acgtggcggt tcggctgggg
    18361 aatgtggctc ccgcgcggcg cggcgagcgc cgcgcggcgc cgccgctgcc cggcattcga
    18421 ggctccggcc cgcatcaggt gctaaagtgt agtgtaacaa aacgtgggtg aactgttcga
    18481 cctggcaatc atcggcggcg gaatcaacgg aacggcgctc gcgcaactgg cagcggataa
    18541 tggctaccgc atcgtgttgc tggaaaaaac agatttcggc gccggcgtca ccagccgttc
    18601 cacacgcctc attcacggcg gcctccgcta tctcgagcac gggcaggtga aggttgtccg
    18661 cgagtctctg cgagaacggg atgcgcttct ccgggacttt ccacaccaag tccggccgct
    18721 gccgtttttg gtgccggtct accgcagcga cttgcgcaag ccctggtgga tcggcctggg
    18781 actggaggcg tactcctgga tcgcccgcag ccgccttttg cctcgccacc agcatctctc
    18841 gccgcagcag gcgttcgaat tagagccggg gttgaagcgg gaaaatctga acgcagcgtt
    18901 tttgtatttc gattgccagg cggtctaccc cgaacggctc gcgctggaga tggccctgat
    18961 ggccgaagag agcggcgctg aagtgcagaa tcacgccgag gtcaccgctt tgctcaccga
    19021 atccaagccc gagctgcgcg tcgcaggcgt tgctgccaac cgccgcgagt atcgcgcgcg
    19081 cctggtgatc aacgcagccg gaccttgggt cgatcacgtc ctgcaattga tgccgcgggg
    19141 agctggccgc ccgcttctga gcctgattgg cggcgctcac atcgtgactg gaagcttccc
    19201 tggcgcgccc tcacatgcga tctaccacga ggctctggca gaccggcgcc cgttctttat
    19261 catcccctgg cgcgggctct ggctcattgg gacgacggaa acgccggttt ccggggatcc
    19321 ggccggcgtt gcgccgagcc acgacgaaat cgtttacctg ctgcgcgaaa ccaacttgct
    19381 gatcccagag gcgaggctga ccccggattg cattctctac gcgtatgccg ggccccggcc
    19441 cctgcttcgc agcgaatccc agccggctca gtccatcacc cgggaccatg cgatttacga
    19501 tcacgaggtc aaagacggcg tggcgggaat cctcaccctt accgggggta agcttactac
    19561 ggcacgggcg tttgctgccg aggcgttggc ccgcatcgcg cagaagctcg ggcggccccg
    19621 gcccgtgcgc cgtccgcggc ccaaacacga ttgggacggg attccctccc ggctcgctga
    19681 aatctatggg ggccgcgcgg ccgacatcgt cgcactcgcg gaaaaaaatc ccgaactgga
    19741 ccggccggtt tgcccgccga agcccaccac cgccgctgag atcgtgcacg cggtgacgaa
    19801 ggaaaaagcc cggaccctgg gcgacattct gttgcggcgc acgggactcg ccttcgagcc
    19861 cgatctgcaa gagcatgaga tcgcccaagt cgcgcacgtt gccgcgcccc tactcggctg
    19921 ggacaacgct ggcatcgcag ccgctctggc cgcgtatcgc aatgaggccg gccgaactct
    19981 ctaccggaga ccggaacggt gatcccggcc gcggcactgg ccgttctcgt ggcgctcgcg
    20041 tggggctgcc tcctgctgag gcgggcccgc ctgtccggtt taagtgaatt tgatgcgatt
    20101 gatactcagg aaccgccgga gaggtggccg ccggtaagcg ttatcattcc cgcgcgcaac
    20161 gaggcagcca tgctgcgctt caccctgcct tccgctttgt ccttctatta tccccagatc
    20221 gaggttcttc ttgtggacga ttgctccaac gatcgtacgg cggcggcggc cgaagaaaca
    20281 gcgcg
//
LOCUS       DASREK010000110         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1875606, whole genome shotgun sequence.
ACCESSION   DASREK010000110 DASREK010000000
VERSION     DASREK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875606"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1035)
                     /locus_tag="VFA54_05495"
     CDS             complement(67..1035)
                     /locus_tag="VFA54_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS50293.1"
                     /translation="MTTQMQTRKQSAAIIVRPMAETDVSEARRIFQRAFGAFIGVPDP
                     DTFWEDRDYVRTRWAGKLGGSLVAEADGCLVGSNFAANWGSFAFFGPLTVHPDRWDQG
                     IAQHLLGPTMELFQSWNVRHAGLFTFAQSAKHVNLYQKFGFWPRFLTAIMTRPSERRP
                     VSWTPFSGLNPESRSHALRACRELSDSIFEGLDLSREIQAIGQLQLGDTLLLWSGDVL
                     DGFACCHCGPGTEAGNDNCYIKFGAIRRTAEADQLFDALLDVCEAFAAERGLARVEAG
                     VNLGRERAHRKLRARGYRTQIQGVAMHRPNDPAFSNAADYVIDDWR"
     gene            complement(1146..2042)
                     /locus_tag="VFA54_05500"
     CDS             complement(1146..2042)
                     /locus_tag="VFA54_05500"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HZS50294.1"
                     /translation="MRLITVDDPNSGQEEFCRALERGDILFFPHTPILGEEDRKFLLG
                     IELTDAGYHKNIAYRPNTGKVTGFAESQALDVARLRSILHAYSERALEFMRELLPRYA
                     AGWRVDFASFRPQEEEGRQLPVKKRNDLLHTDAFPTRPTRGDLILRFFTNIHPEKSRV
                     WLTGDPFALLAPQYAEDAGLRRLAAQAESPWTSLQRGLVGMLRAAGLPVVDRTPYDRF
                     MLRFHDYLKYNREYQEKSVKYMFEFPPGSTWMVFTDIVPHAVLSGRYALEQTVIVARR
                     SLASPESAPVAILEKLSQSRLA"
     gene            complement(2135..>2607)
                     /locus_tag="VFA54_05505"
     CDS             complement(2135..>2607)
                     /locus_tag="VFA54_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZS50295.1"
                     /translation="ISYPSMWGATNWMAPSYDPATGWLYIAFREFGDRYYKQPADYEA
                     GKAYLGGKTDPGPDQEWGGVKAINPETGNIVWEQKFFLGSLSAGVLATGGGVLFAAGR
                     DGNLIAFESRTGKPLWRFQTGAPINSSPMSYAVDGKQFIALAAGNVLYSFALPE"
BASE COUNT          544 a          800 c          772 g          491 t
ORIGIN      
        1 gggttcgaat ccctccgggc gcgccagctt aaacttctgg aggccagtgc tgcggtcccg
       61 gcggctctaa cgccagtcgt caatgacgta atccgccgca ttgctgaacg cggggtcgtt
      121 gggccgatgc atcgcgacgc cttgaatttg ggtccgataa ccgcgggcgc gcagcttgcg
      181 atgggcgcgc tcccgcccta aattgacgcc ggcctcgaca cgcgccaagc cccgctctgc
      241 cgcaaacgcc tcgcaaacat ccagcaaagc atcgaaaagc tgatccgctt cggctgtccg
      301 gcgaatggcc ccgaatttga tgtagcagtt gtcgtttccg gcttcggttc cgggcccgca
      361 atgacagcac gcgaatccgt cgagcacgtc gccgctccat agaagaagcg tgtccccgag
      421 ctgcaactgg ccgatggcct gaatctcccg gctgagatcg agcccctcga atatggaatc
      481 agagagctcg cggcaagctc ggagggcgtg gctgcggctc tctgggttca aacctgaaaa
      541 cggggtccac gagaccggcc ggcgctccga aggccgcgtc atgattgcgg tcaggaaacg
      601 cggccagaat ccaaattttt gatagaggtt gacgtgtttt gcgctctggg cgaacgtaaa
      661 gagtcccgcg tggcggacat tccaggactg aaacagctcc atcgtcgggc ctaagagatg
      721 ttgcgcgatg ccctggtccc atcgatccgg atggaccgta aggggtccga agaaggcaaa
      781 actgccccaa ttagcggcga agtttgagcc gacaaggcag ccatcagcct ccgcaaccaa
      841 tgagcctccc agctttcctg cccaccgtgt gcggacgtaa tcccgatctt cccaaaatgt
      901 gtcgggatct ggaacgccga tgaaagcgcc gaacgcccgc tgaaaaatac gccgtgcctc
      961 agaaacatcc gtttcggcca tgggacgaac gatgatggcg gcgctctgct ttctcgtttg
     1021 catctgtgtg gtcacggatt ccccctatcg cgcgcatcct aacacaagcg caagcgccag
     1081 ccacgaggct ggccacttgg atccggctct tttgcttgac tcgccagaac cttgagactg
     1141 aaaagttacg ccagccttga ttggctcagc ttttcgagaa tcgcaacggg cgcgctctcg
     1201 ggcgaggcca gactccggcg cgccacaatc acggtctgct cgagcgcata gcgtcccgag
     1261 agaaccgcgt gcgggacgat atcggtgaag accatccagg tcgagcctgg cggaaattcg
     1321 aacatatatt ttacagattt ttcttgatat tcccggttgt acttcaagta atcgtggaag
     1381 cgcagcataa agcggtcgta aggcgtgcga tcgacgaccg gcagacccgc cgcacgcagc
     1441 atccctacca gtccgcgctg caaggaagtc caaggcgatt cggcctgcgc cgcgagccgc
     1501 cgcaacccgg catcctctgc atactgaggc gccagaagcg caaacggatc cccggtgagc
     1561 cagaccctcg atttctcagg atgaatattg gtgaaaaacc gcagaatcaa atcaccgcgg
     1621 gtgggacgtg tgggaaaggc atcggtgtgc agcaggtcat tgcgcttttt gaccggcaat
     1681 tgccggccct cttcttcttg cgggcggaag ctggcaaaat ccacacgcca gccggccgcg
     1741 tagcggggca ggagttccct catgaattcc aaagcccgct cggagtaggc gtgcaggatg
     1801 gaacgcagtc tcgccacatc cagcgcttgc gattcggcga agccggtcac ctttccagtg
     1861 ttcggccgat aggcgatatt tttgtgatag ccagcatcgg ttagctctat gccgagcagg
     1921 aatttgcggt cctcctcccc cagtatcggc gtgtggggga agaacagaat atcgcctcgc
     1981 tcgagcgcgc ggcaaaattc ctcttggccg ctgttcggat cgtcaacagt aatcaatcgc
     2041 atggcatcac atgccggcca ggcacatggc ccggaacctg gcgggaatgc cgggaaagcg
     2101 cgccgcctgg gggcgaaggc cctggggagc gtccctactc gggcagcgcg aaactgtaaa
     2161 gcacattgcc cgcagcgagc gcgatgaact gttttccatc gactgcgtaa ctcatgggcg
     2221 acgaattgat gggcgcgccg gtttgaaaac gccacagggg cttgccggtc cgcgactcaa
     2281 acgcgatcag attgccgtcg cggccggcgg cgaagagcac gccgccgccg gtggccagta
     2341 cgccggccga aagggagccg aggaaaaact tttgttccca cacaatgttt cccgtctccg
     2401 gattgatcgc tttcacgccg ccccactcct gatccgggcc gggatcggtc ttaccgccca
     2461 gatacgcctt gcccgcctcg taatcggcag gctgtttgta atagcggtcg ccgaactcgc
     2521 ggaaagcgat gtacaaccag ccagttgccg gatcgtacga gggcgccatc caattcgtcg
     2581 cgccccacat gctcggataa ctaatgt
//
LOCUS       DASREK010000111         3832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_226271, whole genome shotgun sequence.
ACCESSION   DASREK010000111 DASREK010000000
VERSION     DASREK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226271"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2660)
                     /locus_tag="VFA54_05510"
     CDS             complement(<1..2660)
                     /locus_tag="VFA54_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS50296.1"
                     /translation="MRKGLWRVFLFFSAAGFACGQVATGNIRGTVSDSTGGVLPNASV
                     VIHSNTTNFERRVSTNERGDFNAPSIPVGVYQITVEASGFQRKVLTDVELQVDQTAII
                     PIELAPGTVTETVEVQASAPLLESQTSSLGQVIEDKQILDMPLNGRNPFALGILAGGT
                     VPFQGLTTNLPIAGGGGRYMANDILLDGVDDNLRNYAGSVGRAGLAYVPSVDAVQEFK
                     VKTNNFSAEYGHSAGYVMNATIKSGANEYHGAFWEFLRNDKLDANNFLSNYSGQPRAP
                     FRQNQFGAAAGGAVRLPFYNGHDRTFFFVDYEGTRIRQKAGSTLNDEPPATFRSGDFS
                     SSSTIIYDPATRVLGPNGVVTDQPFQGNMIPSSRLDPAAVKYQSLIPTPNVGGANSTG
                     PNYLAVTPTKTDRNQGDVRIDHKITDQNSLLARVSVSRADQPSQGAYIYSPTDQVFNT
                     VNAVLGDTHLFSPTFINEFRFGFNRANDSRLATEVDKATAFAAQNGFQSGQIIGFPNV
                     NWTYSGQTLGSTEFSSFGAASTNYAFENSFQWTDNITLIRGAHTIKAGADLRRFRFDR
                     LQGYPPSGNYYFGATYTSNPSLPQTGGLPYADFLLGLPTSVINSSQIAWSMQRDLYFG
                     PFVQDDWKVNSRLTLNLGFRYDLYTQPVQARDTGGMFDPYAVSSTGRKGIIIVPGKNG
                     FSRAIVDGHHRNFGPRFGFAYQATRKFVIRGGYGIFYSQREQNDQTTDMALSLLNFRN
                     IDMPPVSASKTVTPPYTFRSPLIVDPLLDPELSKFTAASPLSSDSGSFNEADIGFSMF
                     PMLQQFNLSLQYELIPNLLVEASYSGARGVHWVQRIDLNQEPFDYALQGINTQANRPF
                     PFLASSEGLDTSDVSTWYNAFNLRVERRYT"
     gene            complement(2720..>3832)
                     /locus_tag="VFA54_05515"
     CDS             complement(2720..>3832)
                     /locus_tag="VFA54_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50297.1"
                     /translation="LRKQLQGAAEPAETALANASRILPVITTAHGASGSNNSYWPEMY
                     MNMPIVDPNRAQPYRDTPDPKVFGTVSAFDPQLFSSVEECAEALANGTMLAKYTPLDV
                     AQWLEDMSAAASTNAAQTLARAADKNAPELRRLLADVAIQAGAGKFFAYKFRSAVLWS
                     LYERSGDRMALEQAIKAYRTAREAWASMAEMAKKVYASDITYGLNANMRGHWYDRLAG
                     IDGDLQDMEKRLAGTRAAAIKSSADPAAIRRAVAAVLSRPQRPSLNGQHTPPPHFEPG
                     KPLELSVSFGGGDSRKVNLLYRHADQSQRWRSAEMQLHDRRYRGVIPADYTQSPYPIL
                     YYFEVHEAGGSGLYPGFSADLSNQPYFLVRSNRRQG"
BASE COUNT          782 a         1112 c         1180 g          758 t
ORIGIN      
        1 tttgtatacc gccgctcgac ccgcaaattg aaagcgttat accaggtgct cacatccgac
       61 gtatccaggc cctcggagct ggcaagaaac ggaaaagggc ggttcgcttg tgtgttgatg
      121 ccctgtagcg cgtagtcgaa aggctcctgg ttcaggtcga tgcgctgcac ccagtgcacg
      181 ccgcgcgcgc cggaatagct ggcctccacg agaagattgg gaatcagctc atactgcaac
      241 gacagattga actgctgcaa catcggaaac atggaaaatc caatatccgc ttcgttgaac
      301 gacccgctgt cggaactaag aggactggca gccgtgaatt tgcttaattc cgggtcgagc
      361 agcggatcga cgataagcgg agaccggaag gtgtacggcg gcgtcacggt tttgctcgcc
      421 gacaccggcg gcatatcgat gttccggaaa ttcagcagcg acaacgccat atccgtcgtc
      481 tgatcgttct gctcccgctg gctgtaaaaa attccataac cgccccgaat cacaaatttg
      541 cgggtcgcct gataggcgaa gccgaagcga gggccgaaat tccggtgatg cccatccaca
      601 atggcccgcg agaatccgtt cttgccgggt acgataatga tgcccttgcg gccggtgctc
      661 gatacggcgt agggatcgaa catgccgccg gtgtcgcgcg cctgaaccgg ctgcgtatag
      721 aggtcgtagc ggaagcccag gttcagcgtg aggcggctgt ttaccttcca gtcgtcctgc
      781 acgaacgggc caaaatacaa gtcgcgctgc atggaccacg cgatctggct ggaattgatc
      841 acgctggtgg gcagcccgag cagaaaatcg gcgtacggca gacctcccgt ttgtggaagg
      901 ctggggttgg acgtataagt ggctccgaaa tagtaattgc cgctcggcgg atacccttgc
      961 agccggtcaa accggaaccg gcgcaggtcc gcgccggctt tgatggtgtg agcgccgcga
     1021 atcagcgtga tgttgtcggt ccactggaac gagttttcga acgcgtagtt tgtgctggcg
     1081 gcgccaaagg agctgaactc ggtcgagccc agcgtctggc cggaatatgt ccagtttacg
     1141 ttgggaaacc cgatgatctg cccggactga aacccgttct gcgcggcgaa ggcggttgct
     1201 ttgtcgacct ccgtagccag ccgcgaatcg ttggcgcggt taaagccgaa gcgaaattcg
     1261 ttgatgaatg tcggactgaa cagatgcgtg tcgcccagaa cagcgttcac cgtgttgaac
     1321 acttggtccg tgggactgta aatataggcg ccctggctcg gctggtccgc gcgggaaaca
     1381 gaaacgcgcg ccagcaagct gttctggtcc gtgatcttgt ggtcgatccg aacatcgccc
     1441 tgattgcgat ccgttttggt gggggtcacc gcaaggtaat tgggcccggt ggaattcgcc
     1501 ccgccgacat tgggcgtggg gatcaaagac tgatacttca ccgccgccgg gtcgaggcgg
     1561 ctggaaggaa tcatgttgcc ttgaaacggc tggtcggtca caacgccatt ggggcccagc
     1621 acccgtgtcg ccggatcata aattatcgtc gaggagctcg aaaaatcgcc ggaacggaac
     1681 gtggcgggag gctcgtcatt caacgtggat cccgctttct gccgaatccg tgtcccttcg
     1741 tagtcaacaa agaaaaaggt ccggtcatgg ccgttgtaga agggcagccg caccgcgccg
     1801 ccggccgccg cgccgaactg gttctgccgg aacggagcgc gaggctggcc ggaatagttg
     1861 gaaagaaaat tgttcgcgtc gagcttgtca ttccgcaaga actcccaaaa cgctccatgg
     1921 tattcgttgg cgcctgactt gatggtggca ttcatcacgt agccggcgga atggccgtac
     1981 tcggcggaga aattgttggt cttgaccttg aattcctgca ctgcgtccac ggaaggaacg
     2041 taggccaggc cggcgcgccc gaccgagccg gcataattgc gcaggttgtc atccacgcca
     2101 tccagaagaa tgtcattggc catatagcgc ccgccgccgc cggcgattgg gagattggtg
     2161 gtcaggcctt gaaacggcac ggttccgcca gcgagaatgc ccaaagcgaa cggattgcgc
     2221 ccgttaagcg gcatgtccag aatctgctta tcctcgatga cctgcccgag cgaggaagtc
     2281 tgcgactcga gcaacggggc cgaggcctgc acctccaccg tctcggtaac cgtgccgggc
     2341 gccagctcga tcggaatgat cgccgtctgg tccacttgca gctcgacgtc agtcaacact
     2401 ttcctctgga agcccgaagc ctccacagtg atttggtaga cgcctaccgg tatcgaaggc
     2461 gcattgaagt cgcctctttc attcgtgctc acacggcgtt caaagttcgt tgtattgctg
     2521 tgaatcacaa ccgaggcgtt gggcaggact ccgcccgtgg aatcggaaac cgttccccgg
     2581 atgttgcccg tggcgacttg gccgcaggcg aagccagcgg cggaaaaaaa cagaaaaacg
     2641 cgccacaacc cttttctcat ttggcccctc cagtccgatg gaaaaaacac ctccagcctg
     2701 ctgagaacct cagcactgcc tagccttgcc ggcggttgct cctcaccagg aaatagggtt
     2761 ggttggaaag atccgcgctg aagcctggat aaagcccgct cccgcccgcc tcgtgcacct
     2821 cgaagtaata tagtatcggg tacggagact gggtatagtc cgccgggatc acgccccggt
     2881 agcggcggtc gtgcaattgc atctcggccg agcgccagcg ctgggactgg tcggcatgcc
     2941 ggtacagcag attcaccttc ctgctatcgc ctccgccaaa ggaaaccgac agttcgagtg
     3001 gtttgccggg ttcgaagtgc ggagggggcg tatgctggcc gttcaacgaa ggtctctgcg
     3061 gccgcgagag cacggcggca acggcgcgcc ggatggccgc tggatcggcc gaggatttga
     3121 tggctgcagc tctggtccct gccaggcgtt tttccatgtc ttgcaagtcg ccgtcgatcc
     3181 cagcaaggcg gtcgtaccag tgcccccgca tattggcgtt caggccatag gtaatatcgg
     3241 aagcgtacac cttctttgcc atttcggcca ttgatgccca agcctctcgc gcggtgcggt
     3301 aggccttgat ggcttgctcg agggccatcc ggtcgcccga gcgctcatag agggaccaca
     3361 agacggcgct gcgaaacttg tacgcgaaaa acttgccggc gccggcctga atggcgacat
     3421 ccgccagcaa gcgccgcagt tcgggggcgt tcttgtcagc cgctcgcgcg agcgtttggg
     3481 ccgcgttcgt ggaagccgcc gccgacatat cctcgagcca ctgggccaca tcaagaggcg
     3541 tgtacttggc gagcattgtg ccattggcca gagcctcggc gcactcctcg acggaggaaa
     3601 acagttgggg atcgaaggcg ctcaccgtgc cgaacacttt gggatccgga gtatcccgat
     3661 acggctgcgc gcggttcgga tcgacaatcg gcatgttcat gtacatctcg ggccagtagc
     3721 tgttattcga acccgaagct ccgtgcgccg tagtaatgac cggcaaaatc cggctggcat
     3781 tggcgagggc cgtctccgca ggctcggcag cgccttgcaa ctgcttccgc aa
//
LOCUS       DASREK010000112         6524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_227144, whole genome shotgun sequence.
ACCESSION   DASREK010000112 DASREK010000000
VERSION     DASREK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227144"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /gene="purL"
                     /locus_tag="VFA54_05520"
     CDS             complement(<1..1172)
                     /gene="purL"
                     /locus_tag="VFA54_05520"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZS50298.1"
                     /translation="MAAITADLISQHQLSEEEYESILRLLGREPTITELGIFSVMWSE
                     HCSYKSSRIHLKKLPTEGPRVLEGPGENAGVVDIGSGYAVAFKIESHNHPSFIEPFQG
                     AATGVGGILRDIFTMGARPIAVMDSLRFGPIGLGPGDASSDAARNRRILEGVVGGIAH
                     YGNCFGVPTVGGECVFEPCYSGNPLVNVFALGVFRRDEIFYARASGIGNPVIYVGAKT
                     GRDGIHGASMASAEFDEQSMEKRPNVQVGDPFLEKLLLEACLEAMQTGAVVGIQDMGA
                     AGLTCSTCEMGGRAGTGIEIDLMHVPQRESGMTPYEIMLSESQERMLLVAERGREEEI
                     FRIFRKWGLDAATIGQVTGDGKLRVKDHGKVVAEIPNAALANQAPLYRRPAAAPFRT"
     gene            complement(1230..1973)
                     /gene="purQ"
                     /locus_tag="VFA54_05525"
     CDS             complement(1230..1973)
                     /gene="purQ"
                     /locus_tag="VFA54_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZS50299.1"
                     /translation="MKFGVVIFPGSNCDHDAYYALAGNLGKNTAYLWHQSEDLQNCDA
                     IILPGGFSYGDYLRTGAIARFSPVMSAVAKFAGSGGLVMGICNGFQILVEAGLLPGAL
                     LRNRNLRFVCRTVHLRTETTKTPFTAELRPGELLAMPVAHGEGSYYCDPATLAGLRRE
                     DRIVFRYASASGEINDEANPNGSLENIAGIVSEGRNVLGLMPHPERAADPLMGSTDGL
                     RIFASMASHLARGARSESVRLAGLVRQTL"
     gene            complement(2147..2368)
                     /locus_tag="VFA54_05530"
     CDS             complement(2147..2368)
                     /locus_tag="VFA54_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50300.1"
                     /translation="MGTFSAGQQVKHERYGFGTVLACDEERISIRFDDHGEKKFVRSM
                     VVLEKSDREPPPEKKSSRRRKTPPAAKQS"
     gene            complement(2565..3599)
                     /locus_tag="VFA54_05535"
     CDS             complement(2565..3599)
                     /locus_tag="VFA54_05535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZS50301.1"
                     /translation="MRILRLSAPLLPALLVLCLSCRKSPDPKVAAVVNGHNITYDELG
                     KYYRAQMQATPEKPDEEQVTMTKLNLLRELIDNQIMLQRAERLGLMAVDADVDAKLTE
                     LKAPYTEEEFQKQLKSRGATVDEIKADLRRSLSIQKLFNKEITAKISISDAEVRAFYE
                     ANKASFNLAEPQVHVLQILVTSIPDPQVRNLRNDKASNEDEARKKIQMIENRLKQGED
                     FVTLAQNYSEDPDSAPNGGDLGFIPQSALDKADAELRRTVALLRPGEISPIVHTPQAY
                     RIIKLQSREPAGQREFSDPRVQQTIRERLLNRKDQLLKAAYYETARNEAKVVNYLAEK
                     VVESAGKAKK"
     gene            complement(3603..4853)
                     /locus_tag="VFA54_05540"
     CDS             complement(3603..4853)
                     /locus_tag="VFA54_05540"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZS50302.1"
                     /translation="MAQIFPFRAYRYSKRAGDPACLLTQPYDKITPEMQQRYLALSPY
                     NVAHIIRGPATPEDDDRNNVYTRAARHLEDWIRCGILVQEQQPLFFAYFQKFELPGKP
                     GRAYLRKGLIALGAVEDYSQGTVYRHELTHSGPKRDRLELLRHTRAHFGQIFMLYDDP
                     QSEVDARLEAAAQAEPLVCVKDEYGAEHSLWPLSRPETIMEIQRLLEPCKLLIADGHH
                     RYETALAFRDENPNLAGARRVMMTLVNMRSPGLAILPTHRVVGNIADFDAERVVREAK
                     RFFSVEEQSSLESLEARMERASPGQTAIGALFRANHKIYLFTRNNEPRLDVSVLHEDL
                     LAKILGLTDEAVREERNIRYIRGFREAARELNAPGAQAVFFLNPAKIDQVAKVAFSGG
                     VMPQKSTDFFPKLLSGLTIYKIEE"
     gene            4878..5630
                     /locus_tag="VFA54_05545"
     CDS             4878..5630
                     /locus_tag="VFA54_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZS50303.1"
                     /translation="MRSASSTPALWIGCCGWPEGRSRYFQHFPAVELQTTFYQPPAVA
                     LAEKWRRDAPAEFAFTLKAWQLITHPASSPTYRRLKDGVEPGKADQYGYFRPTEEVWA
                     AWRATSAVAQALRAHAIVFQCPASFTPTEENKKNLAAFFRAIERGPWLAVWEPRGQWE
                     SPEIRDLCRRLDLIHCVDPFSAEPLHGKAVYFRLHGRGAYRYQYSDLDLRHLHQICAR
                     HLTAGRTPIWVMFNNTFMRDDALRFRDLMAAG"
     gene            5733..6398
                     /locus_tag="VFA54_05550"
     CDS             5733..6398
                     /locus_tag="VFA54_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50304.1"
                     /translation="MSKRSTTGTCNLCGAVFDRTAMGQHLAACKGPAPVPGASRPLTS
                     SFHLVVEASDRDAYWLHVAAPVDAPLSKLDDFLREIWLECCGHVSRFSIDGVEYWSDP
                     DSSSEKSMRAPIGKVMGIGDKVSYEYDFGSTTKLALKVARAFQHPGPAKNVQLLARNE
                     APQIACNHCQEGGLATQICTECQWAGAGWLCAQCAKKHPCPADSFLPVVNSPRVGVCG
                     FTG"
BASE COUNT         1267 a         1974 c         1904 g         1379 t
ORIGIN      
        1 gtgcggaacg gggcagcggc tgggcggcgg tacagcggag cttggtttgc caacgctgcg
       61 ttgggaatct ccgcaacgac cttgccgtga tccttgacgc gtaatttgcc gtcgcccgtc
      121 acctggccga tagtggcggc atcgaggccc catttccgaa atatgcggaa gatttcttcc
      181 tcgcgtcccc gttcggccac cagcaacatc cgctcctgcg attcggagag cataatttca
      241 tagggtgtca tcccgctttc gcgctgcgga acatgcatca aatcgatctc aatgcccgtt
      301 cccgcccgtc cgcccatctc gcaagtcgag cacgtcaatc ccgccgcccc catgtcctgg
      361 atcccgacaa cggccccggt ctgcatggcc tccaggcacg cttccaggag cagtttttcc
      421 aggaaggggt cgcccacctg cacgttcggc cgcttttcca tcgactgctc atcgaattcg
      481 gccgaggcca tcgatgcccc gtgaatgcca tcccggccgg tcttggcgcc gacatagatc
      541 acgggattgc cgatgccgga ggcgcgcgcg tagaagattt catcacgccg gaagacgccc
      601 agcgcgaaga cgttgaccag aggatttccg ctgtagcagg gctcgaaaac gcactcgccg
      661 cccacggttg gcactccaaa gcaattgccg taatgggcga tgccgccgac cacgccttcg
      721 agaatgcgtc ggttgcgggc ggcgtcggac gatgcgtccc ccggcccgag cccgatgggc
      781 ccgaagcgaa gcgagtccat gacggcgatc ggccgcgctc ccatggtgaa gatgtcgcgc
      841 agaatgccgc cgactcccgt cgcggccccc tggaacggct cgataaagct gggatgattg
      901 tgggactcga tcttgaaagc cacggcgtag ccggatccga tatccaccac gccggcattt
      961 tcgccgggcc cttccagcac gcgcgggccc tcagtaggaa gcttcttcag atggatgcga
     1021 gagctcttgt acgaacagtg ttcgctccac atcacgctga aaattcccag ttccgtgatc
     1081 gtcggttcgc ggccgagaag ccgcaaaata ctctcgtatt cctcttcgga aagctgatgt
     1141 tgcgaaatga ggtcagcggt gatggcggcc atcgctacac actggcaaaa gcccggttcg
     1201 ctgcgaaagg atactagcgc tgttgctgct cataaggtct gccggaccaa ccccgccagg
     1261 cggacgcttt cgctccgggc gccgcgcgcc aggtggctgg ccatggaagc aaatatccgc
     1321 agcccgtcgg tgcttcccat cagtggatct gcggcgcgct ccgggtgggg catcaaaccg
     1381 agcacgttcc ggccctcgct gacgattccg gcgatgtttt ccaaagatcc gttgggattg
     1441 gcttcgtcgt taatctcgcc ggacgcggaa gcgtaccgga atacgatccg gtcctcccgc
     1501 cgtaagccgg ccagcgtggc cgggtcacag taatacgagc cttcgccatg ggccaccggc
     1561 attgccagca actcgcccgg ccgaagttcc gctgtaaagg gcgttttagt ggtttcggtc
     1621 ctgagatgga ccgtccggca tacaaaccgc aggttccgat tcctcagcaa tgcgcccggc
     1681 agcaatcctg cctcgaccag gatctggaag ccgttgcaaa tccccatcac cagcccccct
     1741 gagccggcga acttcgccac cgcgctcata actggcgaaa agcgcgcgat cgccccggtt
     1801 cgaagatagt ccccataaga aaagccgccg ggtaggatga ttgcgtcgca gttttgcaga
     1861 tcttctgatt ggtgccacag ataggcggtg ttcttgccca ggtttccggc cagggcgtaa
     1921 taagcgtcgt ggtcgcaatt ggatccagga aaaataacaa cgccaaattt catagggagg
     1981 gctgactcct gatcttaaca tttcacggca cgagcgagaa ggaatctttc gggaaatcag
     2041 aagttctgga gcatcgatct cctagtggaa tccggaacgc aagcgcactc aggaatttgc
     2101 ctttccggcg cgttgctcgc agcggatacg aaaaacggca gcgtttttac gactgcttgg
     2161 ctgctggcgg cgttttgcgg cggcgagagg atttcttttc aggcggcggt tcccgatcgc
     2221 tcttttccag aactaccatg gagcgcacaa attttttctc cccatggtca tcaaaccgaa
     2281 tcgaaattcg ttcttcgtca caagcaagaa ccgtgccgaa gccataacgc tcgtgcttca
     2341 cttgctggcc ggctgaaaaa gtacccatgt acaaattata gcatcctgcg cgcttgcgcg
     2401 ccttcgcgga agccggctgg ttccctcacc gccgcggccg agcctgtatc ggagcgctcc
     2461 ctcgcatacc tgtcgattcc tgggccgatc acctttgcaa gcggctccgg tcgcgaaaca
     2521 acaaactgac cctcagtcag ataggccgct gccggcttcc gcgcctactt cttcgctttg
     2581 ccggcgcttt ccacgacttt ctcggcaagg tagttcacca ccttggcttc gtttctggca
     2641 gtttcgtaat aggcggcctt gaggagttgg tctttacggt tcagtaatct ctcgcggatg
     2701 gtttgctgca ccctgggatc cgaaaactcc ctctggccgg cgggctctct ggattgcagc
     2761 ttgatgatcc gatacgcctg cggcgtatgc acgatgggtg aaatttctcc cgggcgcaac
     2821 agcgcgacgg tccggcgaag ctcggcgtcc gctttgtcga gcgcagattg cggaatgaag
     2881 ccgagatcgc ccccgttcgg cgccgaatcg ggatcttcgg aataattctg cgccagagtg
     2941 acgaaatcct ccccctgctt gaggcggttt tcaatcatct ggatcttctt gcgggcttcg
     3001 tcttcgttgc ttgctttgtc atttcgcaaa ttgcgcacct gggggtccgg aattgacgtc
     3061 accagtatct gcaacacgtg cacctgcggc tccgcgagat taaagctggc tttattggcc
     3121 tcgtaaaatg cacggacttc cgcatcgctg atcgagatct tggcggtgat ctctttgttg
     3181 aagagcttct ggatgctgag gctccgccgc aaatcggcct tgatttcatc caccgtggcg
     3241 ccgcggcttt tcaattgctt ttgaaactct tcttcggtgt aaggcgcctt gagctcggtg
     3301 agcttggcgt ctacatcggc atccacagcc atcaggccca gccgttcggc ccgttgcagc
     3361 atgatctggt tgtcgatgag ctcgcgcagc aggttgagct tggtcatggt tacctgctct
     3421 tcgtcgggct tttccggcgt ggcctgcatc tgcgcccggt aatattttcc cagttcgtcg
     3481 tatgtgatgt tgtgaccgtt caccactgcc gctaccttcg ggtcgggcga ttttctgcaa
     3541 ctcaggcaca aaacgagcaa ggcgggcaac aaaggagcag aaagccggag aatacgcacc
     3601 tgttactcct cgattttgta tatggtaagc ccggagagca gtttcgggaa aaaatcggtc
     3661 gatttctgcg gcatcacgcc gccggaaaac gccactttcg ccacctgatc gattttcgcc
     3721 ggattcagga agaataccgc ctgggcgccg ggcgcgttca actcgcgggc cgcttcgcga
     3781 aagccgcgaa tgtagcggat gtttctctcc tcccgcaccg cctcgtcggt cagcccgagt
     3841 attttggcga gcaggtcttc atgcagcacg gaaacatcca ggcgtggttc gttgtttctt
     3901 gtaaagaggt agatcttatg gttggcgcgg aacagcgctc cgattgcggt ttggccgggc
     3961 gaagcccgct ccatacgggc ctctaaagac tcgagggacg actgctcttc cacagaaaaa
     4021 aacctcttcg cctcgcgaac tacgcgctcc gcgtcgaaat cggcgatatt gccaaccacc
     4081 cggtgcgtcg gaaggatcgc cagcccgggc gaacgcatat tcaccaacgt catcatgacc
     4141 cggcgggcgc cggccaggtt cggattctca tcgcgaaagg ccagagccgt ttcgtagcgg
     4201 tggtgcccgt cggcgatcag cagcttgcag ggttcgagca aacgctggat ttccatgatc
     4261 gtctcaggac ggctgagggg ccataagctg tgctccgccc catattcatc tttcacgcaa
     4321 accaaaggct cagcctgggc ggcggcctca aggcgggcgt ctacctcgga ttgcggatcg
     4381 tcgtacagca tgaagatctg gccgaagtgg gcgcgcgtat ggcgcagcaa ctccagccga
     4441 tcacgcttcg gaccggaatg ggtgagctcg tggcggtaaa ccgtcccttg agagtagtct
     4501 tcgacggcgc cgagcgcaat caggcccttg cgcaggtagg cccgtccagg cttgccgggg
     4561 agctcgaatt tttggaaata ggcgaaaaac aagggctgct gctcttgcac caggatgccg
     4621 cagcggatcc aatcttccaa atgccgggcc gcccgcgtgt aaacattgtt tctgtcgtcg
     4681 tcctccggcg tggcaggccc gcgtatgatg tgtgctacgt tgtaagggct cagcgcaaga
     4741 tagcgctgct gcatttccgg cgtaatcttg tcgtaaggct gggtcagcag gcaggccgga
     4801 tccccggcgc gtttggagta gcggtacgcg cgaaacggaa aaatctgggc catgagaaaa
     4861 ttccagcata ccaaaggatg cgatctgctt cttccacacc cgccctgtgg atcggatgct
     4921 gcggctggcc ggagggccgg agccgatact tccagcattt tcccgccgtg gagctgcaaa
     4981 cgacgtttta tcaaccgccc gccgtcgccc tggcggaaaa gtggcgccgc gacgccccgg
     5041 cagagtttgc ttttacatta aaggcctggc agctcatcac gcatccagct tccagtccaa
     5101 cctaccgccg gctgaaagat ggggtcgagc cgggcaaagc ggatcaatac ggctattttc
     5161 ggcccactga agaagtttgg gcggcgtgga gagccacctc cgcggtcgcg caagcgctgc
     5221 gtgcgcatgc catcgtcttc caatgccctg catcgttcac ccccactgag gagaataaga
     5281 aaaatctcgc cgcctttttt cgagccatag agcgcgggcc gtggctggcg gtttgggagc
     5341 cgcgcgggca gtgggaatcc ccggaaatcc gggatctgtg ccgccgcctg gatctgatcc
     5401 attgcgtgga tcctttctcg gccgagccgt tgcacggaaa ggcggtgtat ttccgactcc
     5461 atggccgggg ggcctaccgt taccaatata gcgatctgga tctgcgccac ttgcatcaaa
     5521 tctgcgcgcg ccacctgacg gcgggccgta cgccgatctg ggtaatgttc aacaacacgt
     5581 ttatgcgcga cgatgcttta cgctttcgag acttgatggc cgcaggctga tcgagccaca
     5641 agcggaaccc gccggctggt ccgtctgccg cctcccaaaa aatttattgg ctgagcaccg
     5701 gcatttctgt gctacacaaa ttaagatcca ctatgagcaa acgatcgacc accggaacct
     5761 gcaatttgtg cggcgccgtt ttcgaccgca ccgcgatggg acagcacctg gcggcctgca
     5821 aaggtccggc tccagttccc ggagcgagcc ggccgcttac ctccagcttt caccttgtcg
     5881 tcgaggcatc ggatagagac gcatactggc tgcacgtcgc cgctcccgtg gacgccccat
     5941 tgagtaaact ggacgatttt ctgcgcgaaa tctggctcga gtgctgcggc cacgtcagcc
     6001 gcttctcgat cgatggcgtt gaatactggt cggatccgga ttcctcgagt gaaaaaagca
     6061 tgcgggcgcc gatagggaaa gtaatgggga tcggcgacaa agtgtcgtat gaatatgatt
     6121 ttggctctac caccaaactt gcgctgaaag tggcgcgggc gttccaacac ccgggcccgg
     6181 ccaaaaacgt gcagctccta gcgcggaatg aggcgccgca gatcgcatgc aatcactgcc
     6241 aggaaggcgg cctggccacc caaatctgta ccgaatgcca atgggccggc gctggctggc
     6301 tgtgtgcgca atgcgcgaag aaacatccgt gccctgctga ttcgttcttg ccggtggtca
     6361 actcgccgcg cgtcggagtc tgcggattca ccggatgaga tggcctgccg gacgcttcct
     6421 cgggcggcgc gccagggtga atagaaggga tcagtttgcc gcggcaagca gattcttcac
     6481 gtactcgtga gccgtatcaa ccaggctcca ggcgccttcc caga
//
LOCUS       DASREK010000113         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1048883, whole genome shotgun sequence.
ACCESSION   DASREK010000113 DASREK010000000
VERSION     DASREK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048883"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            675..1154
                     /locus_tag="VFA54_05555"
     CDS             675..1154
                     /locus_tag="VFA54_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50305.1"
                     /translation="MKILIADDEPVSRRMLEWLVRKNGDEPVVLANGSLAGWLLQRDD
                     APRVAILDWLMPGLDGIQICRLLRSRTDRPYTYILLVTSKTQKEDLVRGLEAGADDYI
                     TKPFDPDELNLRINVGRRVAEVQDRLLSQINDLRRRLAVAEPAGRFAARAACLNPLV"
     gene            1245..2300
                     /locus_tag="VFA54_05560"
     CDS             1245..2300
                     /locus_tag="VFA54_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZS50306.1"
                     /translation="MRAKYLAAACLLVVAACNRRPAHRRLTLAVIPMGTTHEYWKSIH
                     AGAVKAAREEGVDLVWKGPQKEDDRAQQIAVVEDMISRHVDGIILAPLDGKGLQNPVS
                     EARRAGIPAVIVDSHLEGDDYVSFIATDNYQGGRLGARRLAETLGDKGSIILMRVLVG
                     VESTMARERGFLDEIHKHSGIRILSDNQYGGITTETAYQTGENLLNRFPDVDGIFCPN
                     ESTTFGMLRALTDAGKAGKVKFVGFDSSEKLIDALRRKQIDGLVLQNPFRMGELGVKT
                     LVRYLHDPQAAIERRIDTGAVVATPANMDTPEIKQLLSPPLDQYLSRSSPDTAAPVLG
                     KQIKRDAAGFDAPVISG"
     gene            2300..>2969
                     /locus_tag="VFA54_05565"
     CDS             2300..>2969
                     /locus_tag="VFA54_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS50307.1"
                     /translation="MPAAYRIQMSGLRKAFGATLALDGVNLSVEPGEVHALIGENGAG
                     KSTLMKILSGVYRPDAGVMLLDGASYAPADPLDARRHGVVMVYQELALAPHLPVAANI
                     LLGVEPKVMGWIRRAELQRAARRALENLGHGEIDLDIPVSQLSMGAQQIVELARALLS
                     DARVVVLDEPTSSLAAADVPVLFAAIRRLREHGVAIIYISHFLEEIEQIAGGFTVLRD
                     GRSVG"
BASE COUNT          558 a          918 c          908 g          585 t
ORIGIN      
        1 tcgcggcccg aagctgtgac ggatgtgcca gcgtatggct ggaatgcgtt atcgcagacg
       61 ccggctacgt tgagctcgac atagtgggcg gcgccggaag tcgagtcggc cagatgcgcc
      121 agcgataacg gccggtgcag atattattag ttgcgttgaa gaaacgaaaa tctttctggt
      181 ttagccggaa agagcgtccc agttccgcag tgttgctgcg tttagcatat aatactggcg
      241 gctcgtctat cgtaggtgga gaccgatcga acttcggccc ccaataaaga aatctgctgt
      301 ttcacgtgtt ctgccggccc cagaccgtgg tccatggacg gctcggcatc acgagcgtcg
      361 cgatcgcgga aagcggaaga gccaggcgag aggcgcgact catatgggcc atgcgtacta
      421 ctccccggga gctggtccgc aggggcgcga gttttggtag cgcgaccccg ctgtcccatc
      481 ccgctccggc ggctctcttt tcattttgtc gggcaagctc ttcattccct gggaagttag
      541 taccctcgca gccggccgcc ctcgggcgtc tagcctcgga acacaagctg ccagggtcat
      601 aaggtgattc cctgaggaag atgagaagtt cgtactgttg cctctgcctt ttctctggcc
      661 tacgctggcc ttctatgaaa atcctgatcg ccgatgatga gcccgtcagc cgccgcatgc
      721 tcgagtggct ggtccgaaaa aacggcgacg agcccgtggt gcttgcgaac ggctccctgg
      781 ccggatggct cctccagcgc gacgacgctc cgcgcgtggc aatcctggac tggctgatgc
      841 ccggcctgga tggaattcag atttgccggc ttttgcggag ccgcacggac cggccgtaca
      901 cgtacatact cctggtcacc agcaagacac agaaggagga cctcgtgcgc gggctcgagg
      961 ccggagccga cgactacatc acaaaaccgt ttgaccctga cgagttgaat ctccggatca
     1021 acgtgggccg ccgcgtggca gaagttcagg atcgtctgct ttcccagatc aatgatctcc
     1081 gccgccgcct cgcggtggca gaacccgccg gccgctttgc ggcccgcgcc gcatgcctga
     1141 atccgctcgt ttgaaattgg accggccccc aagcgccggc cgctgttcgc atcttcgcac
     1201 agccgggtga gctgcgctcg cgctttgcta caatcagggc gcatatgcgc gcaaaatacc
     1261 tggccgcggc ctgcttgctg gttgtggccg cctgcaatcg ccgtcctgcc catcgccggc
     1321 tgactctggc ggtcatcccg atgggaacta cgcacgaata ttggaagtcg attcatgccg
     1381 gcgcggtaaa ggccgcccgc gaggaaggcg tcgatctggt ttggaaaggc ccccagaaag
     1441 aagacgaccg cgcccagcaa atcgccgtcg tcgaagacat gatttcgcgc cacgtcgatg
     1501 gcatcatact cgcccctttg gacggcaagg ggcttcagaa tccggtctcc gaggcgcgcc
     1561 gcgcgggcat tcccgccgtc atcgtcgatt cccatttgga gggcgacgat tatgtcagct
     1621 tcatcgcgac tgacaattac caagggggcc ggcttggcgc gcggcgcctg gcggaaacgc
     1681 tcggggataa aggatccatc attctaatgc gcgtgctggt cggcgtggaa agcaccatgg
     1741 ctcgcgaacg aggcttcctc gatgaaattc acaagcattc gggcatccgc attctcagcg
     1801 ataatcaata cggcggcatc acgaccgaga ctgcgtacca gacgggcgaa aatctgctca
     1861 accgttttcc cgatgtagac ggcattttct gccccaatga atccacaacc ttcggcatgc
     1921 tgcgggcttt gacggacgcc ggcaaggcgg gcaaggtgaa attcgtgggc ttcgattcga
     1981 gcgaaaagct gatcgacgct ctgcgccgga agcagatcga cggcctggta ttgcagaatc
     2041 cctttcgcat gggcgagctc ggcgtgaaga cgctggttcg ctatctgcac gacccgcagg
     2101 ctgcgatcga gagacgtatc gacaccggcg cagtcgtggc aacgcccgcc aacatggaca
     2161 cgccggagat caagcagctt ctctcaccgc ctctggatca atatttgagc cgttccagcc
     2221 cggacaccgc cgctccagtc ctggggaagc aaatcaaacg cgatgcggcc ggttttgatg
     2281 cgccggtgat ctcgggctga tgcctgccgc atatcgcatc cagatgagcg gcctgcgcaa
     2341 ggcctttggc gcaaccctcg ccctggacgg cgtcaacctc tccgttgagc cgggcgaagt
     2401 gcatgccctc atcggagaaa acggggcggg caaaagcacc ctcatgaaga tattgagcgg
     2461 cgtctatcgc cccgatgccg gcgtcatgct gctcgacggc gcttcctatg cgcccgccga
     2521 tcctctggac gcccgccgcc acggcgtggt gatggtctat caggaactgg ctctcgctcc
     2581 ccatctgccc gtcgctgcca atatcctgct cggggtggaa ccaaaggtta tgggttggat
     2641 tcgccgggcc gagttgcagc gggcggcgcg ccgcgctctc gagaacctcg gccacggaga
     2701 aatcgatctc gatatcccgg tttcgcaact gagcatggga gcccagcaga ttgtcgagtt
     2761 ggctcgcgct ctgctctcgg atgcccgcgt ggtcgtactc gacgagccaa ccagcagtct
     2821 ggcggcggcc gatgttcccg tgctgtttgc cgccatccgg cggctccggg agcacggcgt
     2881 ggcgattatt tacatcagcc atttcctcga ggaaatcgag caaattgccg gtggttttac
     2941 cgttctccgg gacggccgct cggtaggcg
//
LOCUS       DASREK010000114        10305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1544276, whole genome shotgun sequence.
ACCESSION   DASREK010000114 DASREK010000000
VERSION     DASREK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544276"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFA54_05570"
     CDS             complement(<1..227)
                     /locus_tag="VFA54_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50308.1"
                     /translation="MPSLKSVWCRMTHRDVAYGGGEYYWCRRCLCRFPVPWKALRENE
                     GAKTRTAGTTPIFSAHPWLPALAHRPSVVLPA"
     gene            complement(721..1155)
                     /locus_tag="VFA54_05575"
     CDS             complement(721..1155)
                     /locus_tag="VFA54_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS50309.1"
                     /translation="MSEKLRPKIRVLTADDSIEIRETLFSFLTKQDQLVVMAQAADGQ
                     EALEQAGRLPVDLAILDIQMPKMSGLEAAAELRKRFPHLRILLMSAYAEPEMLRAAKK
                     SGANGFIVKGRLRSELMRKIRRLFPEYFPQRLAKKSRPGPQD"
     gene            1720..2832
                     /locus_tag="VFA54_05580"
     CDS             1720..2832
                     /locus_tag="VFA54_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZS50310.1"
                     /translation="MKKRGDTVVGSGRVTPARSLLAGIVESSTDAVIGMRLNGAIITW
                     NSAAERLYGYRAEEIRGKPASILFPSGRGKELSQALRRLKRGQRVHYYETTQLRKDGS
                     EAQVGIAMSPIRNRAGEIVGASAIARDISDRKRTENVLRQLSARLLKVQDQERRRIAR
                     ELHDSTAQRLARLEFCLRSVLQTPLGAETRRTLTECRRLAAECSNEIRTLSYLLHPPL
                     LDELGLGSALRAYVEGYNRRYGMRVALKMPRKRMPLPEDLEMTLFRIAQEALTNIYRH
                     SGTRKATIRLAMESGQVLLEVADQGRGMPAEMVERLNKGTAMLGVGIAGMRERVRQLG
                     GSLEIRSGTRGTTVRAVLPQPSDLKPPEASSVRRAA"
     gene            complement(2847..3716)
                     /locus_tag="VFA54_05585"
     CDS             complement(2847..3716)
                     /locus_tag="VFA54_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50311.1"
                     /translation="MTQPVRAQGAALPKSLAGISVTLRQVSDIPVPLFEVRPIFTCLN
                     PLPGNQGSCGSVTAITVQIPYELRPLCPVCASPLPLNAQLFVTENGNAGGLIDLTPLA
                     DQIHIVTTCDTIVPGSTGRASITGLPCPPMVTHGDGALVSAANPAKPGEELVAYAVGL
                     GATDPPAATGQPATTAARTASSLRMGFNFLPNALPAKPTPLPSPRAAPVTDPFPISGL
                     TPVPIFSGLTPGYVGLYQINFLAPAVPAGTPACQDPNRVAPGADFVQSNVTVSFGSVF
                     SFDGAGICVAVPN"
     gene            complement(4026..4102)
                     /locus_tag="VFA54_05590"
     tRNA            complement(4026..4102)
                     /locus_tag="VFA54_05590"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4066..4068),aa:Asp,seq:gtc)
     gene            complement(4208..4840)
                     /locus_tag="VFA54_05595"
     CDS             complement(4208..4840)
                     /locus_tag="VFA54_05595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HZS50312.1"
                     /translation="MNWLGSFLIAVLGIAISIAAMGQKTPPTRQQVLDHLARMRATEK
                     GMMNVDPREGEYLSNLVKKLNAKHVLEIGTSNGYSGIWLALGLRETGGKLLTLDVDQG
                     RRSLALKNFRATGVEDIVESRLGDALKILPGLQGPFELVFIDAWKPDYKKYLDLVLPL
                     VPPGGVIVAHNVASHPDEMPDFLQAIRTNPQLRTEFVNIGPGGFSVSYKK"
     gene            complement(4850..6907)
                     /locus_tag="VFA54_05600"
     CDS             complement(4850..6907)
                     /locus_tag="VFA54_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS50313.1"
                     /translation="MRPWLLSLLCVSLACANAQAYVRERTDPNGPFLFRTDNNNIQFR
                     VNDQTAAGLTNSSGGTTITADSAPIQALQAATAAWSSIGTASITFAPLQKTSVSNSSR
                     DFINLITFADTPANRSILGDALAVTNPVGVRTTGEMVDTDILFNPKLTYSTTFQGGTY
                     DIQTIATHELGHTLGADHSGLLGATMFFAARAASGILSALTSDEIAFATKVYPQANAA
                     ASFGSISGKITLDNGQSATDALVAAVNPSTGVAVGGVTDASGAYTIEMIPPGQYFVYA
                     EPMDGPVMPDQFATIAANPNTSFTTTFLGGNDSPQKLTVSAGQTASADLTVPSGTPAM
                     NITLGAAATGQIASVNGGASALYPGQTFNFAIAGQGLDDPSINENSISFLGGPISMQG
                     GSLSRSRTQSTNTPILLFTVNVAPNAQPGLYTLVIHSASGTAVYSGGAKILPPAPAFS
                     AKGVVNGASFLGYGVTPGEIVSIFGAGLGPAAGVSGGIDPITGGLAASVGGVTVTFNG
                     VPAPLFYASQNQLNVQVPFEVAGQAGANISVRYQASVSSPPVNVPVTSASAGIFQLPG
                     SSQGVILNQDNTVNSPSNPAARGSVVQVYATGQGAVQPPLTTGQLAPALGPLSNAQQT
                     VTATIGGQTADVRFAGMAPNFVGLLQVNVAVPSGAPQGSSVPIMLNIGGALSQPNVTL
                     AIQ"
     gene            complement(6913..7485)
                     /locus_tag="VFA54_05605"
     CDS             complement(6913..7485)
                     /locus_tag="VFA54_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50314.1"
                     /translation="MKCRPLLQLGILLLCAAWPAFSTMVPRFSLEELATGSDLIVHGK
                     VLRTWTAWDAATQIIWTHSEVQVIDALKGSPQSAVVVSEPGGSVDDVTMAISGMPRYQ
                     PGEEVVLFLFQTPVGLWRARGLGQGKFLVTESVSGVRTVRPSPNGALVVEPIGGKPTG
                     GTDLRQLRGMRLEDFKSRVRAAALLRKGTN"
     gene            complement(7566..8177)
                     /locus_tag="VFA54_05610"
     CDS             complement(7566..8177)
                     /locus_tag="VFA54_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50315.1"
                     /translation="MSSRAQNLFAVTYRNVPGKGVFAQGGLKFQTEESAMRRQYAAVL
                     RTAPLEVLLGEASLWVLWPSTAAIWAFPLLLWRFSTDVAVLAAIGLFLAVQIAHMLFY
                     RQWLNYAGFVLGNRALQGLVYAGSAAGFWFSGQWMKALALAAWLLLMATGVVQVVFTV
                     PFLPLLTRLFSARPADQALWNVACTHARRTGIPVRDLQSAPKR"
     gene            8216..>10305
                     /locus_tag="VFA54_05615"
     CDS             8216..>10305
                     /locus_tag="VFA54_05615"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZS50316.1"
                     /translation="MTRLGPCAAAAALSILSIACGAARPGLEQRAGEALSQTEGRIQL
                     DGLAKPVEVLRDTWGVPHIFAQSAADLFFAQGFVAAQDRLFQMEMWRRAGAGELSEIL
                     GPSYLERDRFARLLRFRGDMEQEWSSYSPDAKELATAFVRGVNAYIEKQGDRLPIEFQ
                     LLGVRPGKWKPEDCLLRLAGLLMCRNAVSEIARARLISRVGPELAAALEPPDPPVKIA
                     ALKDPLMEGIDESVTKTLREAIGPVRFDNEQGSNNWVVDGTLSATGKPILANDPHRPV
                     ILPSLRYMAHLVAPGWNVIGSGEPALPGIAVGHNERIAFGFTIVGIDQQDIYVERTNP
                     SNANLYRWRGNWRPMEVQHEKIAVKGRAEPTDVMLKFTVHGPVIYEEPGKGRAFALRW
                     VGSEPGTAGYLASLAADRAQNWQEFTRAMERWKVPSENLIYADVDGNIGWIAAGLAPI
                     REKGSGLLPAPGEAGDYEWSGYLPLSEHPQLYNPPQHYIATANHNILPKGYPHHLGYE
                     WTPPFRYRRIDQVLKSGRKFTVEDFEKLQTDVTSVEAQEFVALLKKAPAGGGPARQLL
                     ESWDGVLGKDSPAAALFEIWARQVKSRFVAARVPADARALAESAMNDRTLLDLLEREP
                     AASRHALLLAALQEAVVEGQKLLGEEDVQAWRWGALHRIRFHHPLALDAERRKFFDLG
                     PVERPGDGYTVCATG"
BASE COUNT         2072 a         3118 c         3198 g         1917 t
ORIGIN      
        1 gcgggaagaa caacggaggg ccgatgcgcc agcgccggca gccacggatg tgcggagaag
       61 atcggagttg ttccggcagt cctggtcttc gccccttcat tctcccgcag agccttccac
      121 ggcacaggga accggcacaa gcaccgccgg caccagtaat actctccgcc gccgtaggcg
      181 acatcccgat gcgtcatccg gcaccatact gatttgaggc ttggcatcct tcctcctgct
      241 gacgaaacaa aagccgctgg ttttcaccca acgcggcgaa caacgcatcg gcggaaaggc
      301 cgcggacttc ccggccgggg gtgagcgcgc ttgtgcagcc aaaattaatc tgatgagaac
      361 agcgcacgtc tcagaatcac tcaagccgcc gcgatttccc aatagctctt tcgtacctgg
      421 catagcccac tcgtaccggt tttccgctat cgcggcgata ctggcagcag cttgctttgc
      481 ggcaaccgct gtcgagcata acagcgcatc actagagcga gaactaggaa tgttcccagt
      541 aaatgcttct atttggcgga gcgaagccgt ttgccgactt gccttttcga cggagaagag
      601 agccttcgat tggccgtgtg cgcaggcgca caaggcagtc gcgttgtgac gctggtggag
      661 aaagtgaatc cggttcctgg cggcggacgc tccgaaagtg caggcctcgg aatatccatt
      721 tcaatcttgc ggcccgggcc tgcttttctt agccagccgc tgcgggaaat attccggaaa
      781 aagccgccga attttccgca tgagctcgct cctcagacgt cctttaacga taaaaccgtt
      841 tgccccgctc ttcttcgccg cgcgcagcat ttcgggctct gcgtaggcgc tcatcagcaa
      901 tatccgcaaa tgggggaagc gtttgcgcaa ttctgcggcg gcttctaatc cgctcatttt
      961 cggcatctga atatccagaa tggcaagatc aaccggcagg cgcccggcct gttccagagc
     1021 ctcctggccg tcggccgcct gggccatcac gaccaactgg tcctgcttgg taaggaagga
     1081 aaacagcgtc tcacgaatct cgatcgaatc gtccgctgtg aggactctga tcttgggtct
     1141 gagcttttct gacactgaac ctcttctcgt cccgaacgaa tttgcagccg gccttttggg
     1201 ggaccacgct ccgacggagc agggcttccg gccaggagaa accggatatc agctttatca
     1261 actgctggaa aaggaaccgc ctgactgcct tcggctacac gcctcctcct atccacaact
     1321 ctggttcagc cgggaaagca aattcaatag atcgggagta ccgcttgcgc gccgtattcg
     1381 taccgttact cttgccgagc caacgatgcg gaaaacggcg tttcgcgggc tcaaagcaat
     1441 ccgagcaggc gcgccttgcg gcggctcgca tgggaagaac cggcggcttc catgctgagc
     1501 cgctcccgtc aggttcgccc gggtgcggca tgttcggtcc gcctggtgac ccggcgatgc
     1561 agccgaagat ttggcgagaa ttgcggcgca ataccgtgtc attttgctcg ggaaatccct
     1621 agtatttggg tgctaaacgg tgtgttattc tgcaatacct cagctagatt cgtcgctctg
     1681 ggggatcgca aggtccgagt tactgtcggt tatgcgtgca tgaaaaagcg tggcgatacg
     1741 gtcgttggaa gcggccgagt aacccccgct cggtcgctcc tggccggcat cgtagagtcc
     1801 tcgactgatg ctgttatagg gatgagactg aatggggcca tcatcacttg gaattccgca
     1861 gccgagagac tgtacggcta cagggcggaa gagatccggg gaaagccggc ttcgattctt
     1921 tttccttccg gccggggaaa ggagctttct caagctttga ggcggctcaa gcgcggccag
     1981 cgcgtgcatt attacgagac tacccaactc cgcaaggacg gcagcgaggc gcaggtgggg
     2041 attgcgatgt ctcccattcg caatcgcgcc ggcgaaatcg tcggcgcctc ggcgatcgcg
     2101 cgcgatatca gcgatcgcaa gcggacggag aacgtcctcc ggcagctttc cgcgcggctg
     2161 ttgaaggtgc aggatcaaga acggcgccgc atcgcgcgcg agctgcacga tagcacggcc
     2221 cagcggctgg cgcggctgga attttgcctc cggagcgttt tgcaaacccc gctgggcgcc
     2281 gagacccgcc ggactttgac cgaatgccgc aggctggcgg ccgaatgctc aaacgaaatt
     2341 cgaacgctct cttatctcct gcaccctcct ttattggacg agctcgggct gggcagcgcc
     2401 cttcgggcct acgtcgaggg ctataaccgg cgctacggaa tgcgggtagc cctgaaaatg
     2461 ccgcgcaagc ggatgcctct gccggaagat ctggaaatga cgctgttccg catcgcccag
     2521 gaagctttga caaacattta ccggcactcg ggaacgcgca aggccacgat ccgtctggcg
     2581 atggaatccg gccaggttct attggaagtg gcggatcagg gccgcggtat gccggccgaa
     2641 atggtggaac gcctgaacaa gggcacggcc atgctcggcg tcggcatcgc cggcatgcgc
     2701 gagagagtgc ggcaactcgg cggatccctg gaaatccgct cggggactag gggaaccact
     2761 gtccgcgctg tgttgcccca gccgagcgat ttgaaacctc cggaggcgtc ctcggtgcgc
     2821 cgcgcggctt agcccgggca taagtctcag ttcggcacag cgacgcagat cccggctcca
     2881 tcgaaggaaa acacggatcc gaagctgact gtgacattgg actgcacgaa gtcggccccg
     2941 ggcgccactc ggttcgggtc ctggcaggct ggcgttcccg ccggaacagc gggcgccagg
     3001 aagttgatct gatacagccc cacgtaaccc ggcgtgaggc cggaaaaaat cgggaccggc
     3061 gtgaggccgg aaatcgggaa aggatcggtt accggcgctg cccgggggct cggcaagggc
     3121 gtcggcttcg ccggcagcgc gttaggaagg aagttgaagc ccatacgcag agacgaagca
     3181 gtccgtgcag cagtggtggc cggttggccg gtcgcagccg gtggatctgt cgcgcccagg
     3241 ccaacggcat acgccacgag ctcctcgccc ggctttgccg gattggccgc tgaaaccagc
     3301 gcgccatccc cgtgggtgac catcggtgga caaggcagcc ccgtgatgct ggcgcggcct
     3361 gtggatccag ggacgatcgt atcgcaggtg gtgacgatgt ggatctggtc cgccaagggc
     3421 gtcagatcga tcaggccgcc cgcattgccg ttctccgtca cgaataactg cgcgttcagc
     3481 ggcagcggac tcgcgcagac cgggcacaga gggcgaagct cgtaagggat ttggacggtg
     3541 attgccgtga ccgatccaca ggaaccctgg tttccgggca gcggattcaa gcaggtgaaa
     3601 atcggccgaa cctcaaaaag gggaacggga atatcggaga cctgccgcag cgttaccgaa
     3661 atgcccgcca gcgatttcgg cagggccgca ccttgagcgc ggacaggctg cgtcaaggag
     3721 cgcccaacac cactcacaaa aagcgtaaga atttgacctg gggccgcagg aatcggcgct
     3781 gggtacaaat agccggcccc tgcaactgtg tttgtctgag cgaacgcgga agtacaccaa
     3841 agaataaggc ctgcaacgat gtgtggtctg cgcatggggc tacctcttga tgaaaggaaa
     3901 cgactcgagg cttctgcctg gggccgagag aacggagacc cgggccggat ccccgccatc
     3961 gaatgacacg ggcgccgatg cggcccaaaa agaaccgcgg aaaagcggcc cagaggttga
     4021 gagaatggcg gggacgacgg ggctcgaacc cgcgacctcc gacgtgacag gccggcgttc
     4081 taaccaactg aactacgtcc ccgctcaggc gggcaaattg tctactgccg atcatgctgc
     4141 gggaagcgca gccgtgacta ttagagtagc atacagccgg cttcatagcc tggaatcgct
     4201 gggccgctta ttttttataa ctaaccgaga aacctccggg accgatattc acgaattcgg
     4261 ttctcaactg cgggttcgtc cggatcgcct gcaagaagtc gggcatttca tcgggatgcg
     4321 aagcgacatt gtgggccacg atcaccccgc ccggaggaac tagcggcaaa accagatcca
     4381 ggtatttctt gtagtccggt ttccaagcgt cgataaacac cagctcgaac ggcccttgca
     4441 aaccgggcaa aatcttcagc gcatcgccaa gccgcgattc gacgatgtct tctacgccgg
     4501 tagctctgaa attcttgagc gccagcgacc gcctgccttg gtccacgtcc agcgtaagca
     4561 gctttccccc ggtctcccgc aggcccagcg cgagccagat tccggagtag ccgttggatg
     4621 tgccaatctc gagaacgtgt ttggcgttca attttttgac caggttggaa aggtattcgc
     4681 cttcccgggg atccacgttc atcatcccct tctcggtggc tcgcatccgg gcaagatgat
     4741 cgagcacctg ctgccgcgtc ggcggcgttt tctgccccat agcggcgatg gagatggcga
     4801 ttcccaagac tgcgatcagg aaagacccga gccaattcat gactcttcct tattgaatcg
     4861 ccaacgtcac gttcggctgg ctgagagcgc cgccgatatt aagcataatc ggcacgctgg
     4921 acccctgggg ggcgcccgat ggtactgcca cattcacctg gagaaggccg acaaagttcg
     4981 gcgccatgcc cgcaaaccgc acatccgccg tctggccgcc gatcgtggcg gtgaccgtct
     5041 gctgcgcgtt gctcagcggc ccaagagccg gggcaagctg gcctgtggtc agaggcggct
     5101 gcaccgctcc ctgccccgtg gcatagacct gaacgacgct gcctcgcgca gcgggattgc
     5161 ttgggctgtt caccgtgttg tcctggttca gaatcactcc ctggctgctg cccggtaatt
     5221 ggaaaatccc tgcactggcg ctggtaacag gcacatttac gggcggcgag ctcacggaag
     5281 cctggtaacg gacgcttata ttcgcgcccg cctgcccggc gacctcaaaa ggaacctgca
     5341 cgttcagttg attctgactg gcgtaaaaca gcggcgccgg cacgccgtta aacgtaactg
     5401 ttacgcctcc cacggatgcc gccagccctc ccgtgatggg gtcgatgccg ccggaaacac
     5461 ccgcagccgg cccgagcccg gcgccgaaaa tcgagacgat ttcgcccggc gttacgccat
     5521 atcccaggaa acttgcgccg ttcaccaccc ctttggcgct gaatgcgggc gccggcggca
     5581 ggatcttcgc tccgccagag tacacggctg tgccagaggc gctatgtatt actaacgtat
     5641 ataagcccgg ctgggcgttg ggagccacgt ttaccgtgaa aagcaaaatc ggggtgttgg
     5701 tgctctgggt cctgctgcgg gaaaggctgc cgccttgcat ggaaatgggg ccgcccagaa
     5761 aggaaatgga attctcgttg atgctggggt cgtcaagacc ttggccggcg atcgcaaaat
     5821 tgaaggtctg tcccgggtac agagccgatg cgccgccatt taccgaagct atttgaccgg
     5881 ttgcggcggc tcccagggtg atgttcatcg ccggcgtgcc cgagggaacc gtcagatcgg
     5941 cgctggcagt ctggccggcg ctgactgtca gcttctgcgg cgagtcattt ccaccaagga
     6001 aagtggtcgt gaaacttgtg ttcgggttgg cggcgattgt agcgaactgg tcgggcatga
     6061 ccgggccgtc catcggctcg gcgtagacaa aatactggcc cggcgggatc atctcgatcg
     6121 tgtatgcccc gctggcatcg gtgacgcccc cgacggcgac gccggtcgag gggttcaccg
     6181 cagccaccaa agcatctgtg gccgactgcc cattatcgag cgtgattttt cccgagatcg
     6241 agccgaacga cgcggcggcg ttggcttggg ggtagacctt cgtggcaaag gcaatctcat
     6301 ccgaggtcaa cgcgctcagg atgccgctcg cggcccgggc tgcaaaaaac atggtcgcgc
     6361 ccagcaggcc ggagtgatcc gcgcccagag tgtgccccag ctcatgcgtc gcgatcgttt
     6421 gaatgtcgta ggtgccgccc tgaaacgtgg tggagtacgt cagcttgggg ttgaagagaa
     6481 tatcggtgtc caccatttcg ccggttgtgc ggactccgac gggattggtc accgccaggg
     6541 cgtctcccaa aatgctgcgg tttgccgggg tatctgcaaa cgtgataagg tttatgaaat
     6601 cgcggctgga gttggacaca gacgtttttt gcagcggggc gaacgtaata gaggcagtgc
     6661 cgatactgct ccaggcggcg gtggcggcct gcaaagcttg tattggggcg ctgtctgctg
     6721 taattgtagt cccccccgag ctgtttgtga gcccggccgc tgtctgatcg tttacgcgaa
     6781 actgaatatt gttgttatcg gtccggaaca aaaacgggcc gttcggatcc gttcgttccc
     6841 ggacatacgc ttgagcgttg gcgcaggcca gagagacaca caggagactc aagagccagg
     6901 gtcgcatagg aatcagttcg ttcccttccg taaaagggcc gctgcccgca ccctggattt
     6961 gaagtcttcc aagcgcattc cccggagctg ccggagatcg gttccccctg tgggctttcc
     7021 accgattggc tcgacgacga gcgctccatt cggcgacggg cgcacggtac gcacgccgct
     7081 caccgattcg gtcaccagga acttgccctg gccgagaccc cgggcccgcc acaggccgac
     7141 cggcgtctga aacaggaaca acacgacttc ttcgccgggt tgatagcgcg gcatgcctga
     7201 gatggccata gtcacatcat ccacgctgcc gccgggctcg ctgacgacga cggccgattg
     7261 cggcgagccc ttcagcgcat cgattacctg cacttcgctg tgcgtccaga tgatttgcgt
     7321 ggcggcatcc cacgccgtcc aggtccgaag cacttttccg tgcacgatca aatccgatcc
     7381 agtcgcgagt tcttcgagag aaaagcgcgg caccatagtg ctgaaagcgg gccatgccgc
     7441 gcataagagc aaaatgccga gttgcaatag cggtctacat ttcataaaag attcgtcttc
     7501 accagccggc gcacaagccg agacccttcc attctaaacg tgatcggccg gacaaaagtt
     7561 tccggttaac gcttgggcgc agactgtaga tctctcaccg gaatcccagt tcggcgcgcg
     7621 tgcgtgcacg ccacattcca caaggcttga tcggcgggac gagcagaaaa caagcgggtt
     7681 agcagaggaa ggaaggggac ggtgaagaca acctgcacca cccctgtggc catcagcaac
     7741 agccatgcag ccaaggccag agctttcatc cattgtccgc taaaccagaa tccagcggcc
     7801 gaaccggcgt aaaccagtcc ttgcagcgcg cggttgccga gcacaaagcc ggcgtagttc
     7861 agccactgcc ggtaaaaaag catgtgggcg atttgcaccg ccaaaaacag cccgattgcg
     7921 gcgagcacgg ccacgtccgt ggaaaagcgc cacagcagca gcgggaaggc ccaaatggcg
     7981 gccgtcgaag gccagagcac ccacagcgac gcctcgccca gaagcacctc gagaggcgcc
     8041 gttcgcagaa ccgccgcata ctggcggcgc atggcgcttt cttccgtttg aaatttcagg
     8101 ccgccctgcg cgaacacgcc cttgccgggc acattccggt aagtcacggc aaacagattt
     8161 tgggcgcggc tgctcattgc atgcacaagc gtccaggatt actatcatag ccgctatgac
     8221 gcgcctgggt ccttgcgccg ccgcggcggc gttgtcgatt ctctccattg cgtgcggcgc
     8281 cgctcggccg gggctcgagc agcgcgccgg cgaagctcta tcacagaccg aagggcgaat
     8341 ccaactcgac ggcttggcca agccggtcga agtgcttcgc gacacctggg gcgtgccgca
     8401 catcttcgcc cagtccgcag ccgacctgtt ttttgctcaa ggattcgtcg ccgcgcagga
     8461 ccgcctgttc cagatggaaa tgtggcggcg ggccggcgcg ggagagctgt cggaaatcct
     8521 cggaccctcc tacctggagc gcgaccgctt tgcccggttg ctccggtttc gcggcgatat
     8581 ggaacaagag tggtcgagtt acagtcccga tgccaaggag ctggctacag cgttcgttcg
     8641 cggcgtgaac gcttatatcg agaagcaggg cgatcgcttg cccatagaat ttcaactgct
     8701 gggcgtcagg ccgggcaagt ggaaaccgga ggactgcttg ctgcgcctgg ccggcctgct
     8761 gatgtgccgc aatgccgtct cggaaattgc gcgcgctcgg ctgatttcca gggttgggcc
     8821 ggaactggcg gcggctctcg agccgcccga tccgccggtc aaaatagccg ctctgaaaga
     8881 tcctttgatg gaaggcatcg atgagagcgt gacgaaaacg ctccgcgagg cgatcggccc
     8941 ggtacgcttt gacaacgagc aaggcagcaa caactgggtt gtcgatggaa cgctctccgc
     9001 cactggcaag ccgatcctcg ccaacgatcc gcaccggccc gtgattctgc cttcgctgcg
     9061 ttacatggcg catctggttg cgcccggatg gaacgtcatc ggctcgggcg agcccgccct
     9121 gcccggaatc gccgtgggcc acaatgagcg catcgccttc ggcttcacga tcgtcggcat
     9181 cgatcagcaa gacatctacg tggaaaggac gaacccgtcc aacgccaatc tctatcgatg
     9241 gcgcgggaac tggcgtccaa tggaggtgca gcacgaaaaa attgccgtaa aaggccgcgc
     9301 cgagccaacc gatgtcatgt tgaaattcac cgtgcacggc ccagtgattt acgaggagcc
     9361 gggcaaaggc cgcgctttcg cgctgcgctg ggtgggaagc gagccgggca cggcgggcta
     9421 cctggcatct ctcgcggccg accgcgccca aaactggcaa gagttcaccc gcgctatgga
     9481 gcgctggaaa gtgccgtcag aaaatctgat ttacgcggat gtcgacggca acattggctg
     9541 gatcgccgcg ggcctggcgc caatccgaga aaaggggagt gggctgctgc ccgcgccggg
     9601 cgaggccggc gactatgagt ggagcggcta tttgccttta tccgagcacc cgcagcttta
     9661 taacccgccg cagcactaca tagctacggc caatcacaac atcctgccca aggggtatcc
     9721 gcaccatttg ggctacgagt ggacgccgcc cttccggtac cgccgcatcg atcaggtgct
     9781 gaagtcgggc cggaaattta ccgtcgagga cttcgaaaag ctgcaaaccg acgtgacgtc
     9841 ggtcgaggcc caggaattcg tggcgctttt aaagaaggcc ccggcgggag gcggcccggc
     9901 gcgccagttg ctggaatcgt gggacggcgt gctcgggaaa gattcgccgg cggccgctct
     9961 ttttgagatt tgggcgcgcc aggtgaaaag ccggttcgtg gccgctcgcg tgccggcgga
    10021 cgcacgcgcc ctggcggaaa gcgcgatgaa cgatcgcacg ctgcttgacc tgctcgagcg
    10081 cgagcccgcc gcatcgcgcc atgccctcct gctcgccgct ttacaggaag ccgtggtcga
    10141 agggcagaaa ctcctgggag aagaagacgt gcaagcgtgg cgctggggcg ctttgcacag
    10201 gattcgcttc catcatcctc tggcccttga tgccgagcgc cgcaagttct ttgacctcgg
    10261 cccggtggag cgccccggcg acggctacac ggtctgcgca acggg
//
LOCUS       DASREK010000115         8538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1050408, whole genome shotgun sequence.
ACCESSION   DASREK010000115 DASREK010000000
VERSION     DASREK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050408"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VFA54_05620"
     CDS             <1..793
                     /locus_tag="VFA54_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZS50317.1"
                     /translation="PCDSQEFAAAVSEHKPGCILVETVANPMLRVTPLDTINEIARRH
                     GAAVVVDSTFATPLLVRPLQLGSDLVVHSLTKYLAGHGDVLGGIVVSREEFRPVLQSI
                     SRAVGGVLGPFEAYLAMRGVKTFPLRMTRQCANACRVAGWLASHPRIARVHFPGDPQH
                     PDAAIVRRLFPPNMYGAMVSFEVKDAGKEQIFRLVDSLKMIVPATSLGDVHSMILYPP
                     MSSHREISPKHRQRMGIGENFLRLSVGIEAPEDIIEDLDQALGLG"
     gene            complement(929..2191)
                     /locus_tag="VFA54_05625"
     CDS             complement(929..2191)
                     /locus_tag="VFA54_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS50318.1"
                     /translation="MAAIRARHKVLGMLFLASIVTYLDRISIAAAAPHMKEDLGLTTA
                     QMGYVFSAFVFAYGIFEIPSGWLGDRFGPRRVLTRVVLWWSAFTAATGAVRSYFSLLA
                     CRFLFGAGEAGFFPNSSCAISRWFPFGERARAQGIVWTASRLGAALTPAIALPLQAWL
                     GWRPVFFLFGATGLLWAIPWYLWFRDYPGQKAGVSAPELLEIGESGPAPHASLSWKVA
                     LSSANLWAVMLMYHTYCYAAYWFFSWMPTYLVKAKGIEAFATYAALPYLMGAAANVAG
                     GYTSDFMVRKVGLKWGRRSVGLVSMASAGGFMLLSIILQNKLLAIIALSLSFAVSDFM
                     LPNCWAVCLDIGKRYAGTVSGAMNMAGQMGSTIAAAAYGSLVEKHGWNLPLAGMAALS
                     LASALIWLRIDPTKQLVPEKQPALAPQS"
     gene            complement(2203..3204)
                     /locus_tag="VFA54_05630"
     CDS             complement(2203..3204)
                     /locus_tag="VFA54_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HZS50319.1"
                     /translation="MRKCVLLLTIACFVLLPLTAQVTLTRQDNTQIDITIDGKPFSTL
                     FFGPNTLKPYMWPLRAADGKIVTRQWPIAEAPGEAHDHPHHRGLWFAHGEVNGLDFWA
                     PETPGASEFKPGRISLEKVVEVKSGKKAGSMVVMFDWKDQSGQTLITETRKTIIYGDP
                     KLRILDFDITLKADQKVVFGDTKEGTFAIRLAQPLCEPAAKPQPGITPGTGLMTTAEG
                     KQGEKNVWGKRSKWVDYAGTLDGEKLGIAIFDHPGNPKHPTYWHSRSYGLFAANIFGE
                     HDFYNDKGRNGSVTLAPGQSLRFRYRVVIHPGDAQEAGIEALYRKYAGGKETGPGTE"
     gene            complement(3347..3742)
                     /locus_tag="VFA54_05635"
     CDS             complement(3347..3742)
                     /locus_tag="VFA54_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HZS50320.1"
                     /translation="MARPNFQSVDEYIASRPEAVRDLLGQVRSAIRKALPGADEVISY
                     QIPAYRLHSRLVIYFAGWKKHYSLHPATGGLAAAFKDELARYKMSKGTIRFPLLRPVP
                     VKLIGRIAKFRAKEVAGREKAKAATAKKP"
     gene            3891..5468
                     /gene="lysS"
                     /locus_tag="VFA54_05640"
     CDS             3891..5468
                     /gene="lysS"
                     /locus_tag="VFA54_05640"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZS50321.1"
                     /translation="MSFENELLEQRYQKIRQLEELGFTAYPRKFAYTHTLGEIWKQYG
                     SASAEDLAAKRVGVRVAGRVLTVRRQGKAGFAHIQQGGDKLQIYVRQDAVGENSFHLY
                     KMLDAGDIIGAEGYLFRTKTNELTVHVERLEFLSKALLAMPEKWHGLHDVETRYRQRY
                     LDLIANPEVRDVFVRRAKIIGALRRTLEAHGYIEVETPMMQPLYGGAIAKPFRTHHNT
                     LDIDLYLRIAPELYLKRLVVGGLDRVFEINRNFRNEGVSTQHNPEFTMLEFYQAYSDY
                     RDLMDFSEVLLREAAMAALDGKTAAAFGEHLIDFSRLARFSMKEAVVEFWPDPGTRPA
                     ISDLSDCARVVELMEAHNRTVEEKIVAPPGATAGELVGLLFEQVCEKRLIQPTIIYDF
                     PVEVSPLSKNKPDDPEWVERFEIYAAGMEIANAYSELNDPQEQRRRFEMQAALRERGD
                     AETHEMDEDYIRALCYAMPPTAGEGIGIDRLTMILTNSHSIRDVILFPLLRPEGDIGI
                     AAQLRKLEEASADGRQR"
     gene            5558..6787
                     /locus_tag="VFA54_05645"
     CDS             5558..6787
                     /locus_tag="VFA54_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZS50322.1"
                     /translation="MAVEFFIARRYLRAKRKQAVISVITAISTIGVAAGVMALIVALA
                     INNGFGEELRNHLLGATSHVNLMEIERGNGIADWRRFLDRFRKVPHVVAIAPALYGQV
                     MLSGPIQAKGAVLKGIDPSSELGIGDLLRSIKQGSIDAILEEKGLPGLIVGRRLADEI
                     GLRMGTVVTIVSPQGELTPFGPAPSYKRFRVVAIFDSGFYDFDSLWTFTSLKAAQQAL
                     SLGDVINAVEFKLDDLYRAEEVSKEIEKIAGPRFSTTTWMEQNRALLHALKMERLVTI
                     VTIGLIEMVAALNILISLVMMVMEKYRDIAILMSMGMRREQVRKIFMLQGTMIGALGT
                     AIGLTLGYTLSWLGQRYHWIHLDSDIYGLSYVPFQPHLYDGAWVAAAAMLVSFLATIY
                     PARSATRIAPVEVLRYE"
     gene            complement(6869..7543)
                     /locus_tag="VFA54_05650"
     CDS             complement(6869..7543)
                     /locus_tag="VFA54_05650"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50323.1"
                     /translation="MNSDQIERRSFLLPFRCNYLLRKPEHPSPGAFFIVALHGHAMTP
                     EQMLWLTVPLVGEEHYAASLQGPYQIWVNRNGEPRADVAFHWSTRFEPEHSWRLHHDM
                     ILNVIAEIGAASERVVLVGFSQSVALNYRFLCTHPGAVRGAIAICGGIPGDWDQRPYQ
                     HSPAAVLHIAAREDEYYPPVVAETFPERLRTRIPDLEFHMLDGGHRIPTAARPIMQAW
                     LARLSR"
     gene            complement(7748..>8538)
                     /locus_tag="VFA54_05655"
     CDS             complement(7748..>8538)
                     /locus_tag="VFA54_05655"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50324.1"
                     /translation="AFALAQEDSPAYLPGAFQLVASALKDEPKVTEAFRTGAGLGWHE
                     HDPGLFEGTERFFRPNYAANLVSSWIPALDGVENKLKAGARVADVGCGHGSSTILMAQ
                     SYPKSKFVGFDYHAPSIERARKKAQEAGVSDRVTFEVARSKDYPGAGYDLVAFFDCLH
                     DMGDPVGAAAHVLKSLAPDGAWMIVEPFAHDRLEENLNPVGRVYYAASTMICTPASLA
                     QEVGLALGAQAGEARLREVVTSGGFTRFRRAAETPFNIVLEARP"
BASE COUNT         1663 a         2672 c         2567 g         1636 t
ORIGIN      
        1 cccatgcgat tcacaggagt tcgcggcggc cgtcagcgag cacaagccgg gatgtattct
       61 cgtcgagacc gtggcgaatc cgatgctgcg cgttacgcct ctggacacga tcaacgagat
      121 cgcccgccgt catggcgctg ccgtggtcgt ggattcgacc tttgcgaccc ctttgctggt
      181 gcgcccgctc cagcttggct cggacctggt ggtgcacagt ctgacgaagt acttggccgg
      241 ccacggcgac gtactcggcg gcatcgtcgt ctcgcgggaa gagttccgcc cggttctgca
      301 atccatttcc cgggccgtgg gcggagtcct tggcccgttc gaggcttacc ttgccatgcg
      361 cggcgtcaag acctttcccc tgcgcatgac gcgccagtgc gccaacgcgt gccgtgtggc
      421 cggctggctc gcctcgcacc cccgcatcgc cagggttcac ttccccggcg atccgcagca
      481 cccggacgca gccattgtgc ggagattgtt tccgccgaac atgtacggag ctatggtcag
      541 ctttgaagtg aaagacgccg gtaaggagca gatcttccgc ctggtcgatt cattgaagat
      601 gatcgtgccc gccaccagcc tcggcgacgt gcattccatg attctctacc cgcccatgag
      661 ctcgcaccgc gagatttcgc cgaagcaccg ccaacgcatg ggcatcggtg agaattttct
      721 ccgcctgtca gtgggaatcg aagctccgga agacatcatc gaggatcttg accaggctct
      781 gggcctcggt tgacgcctgc tccgcgcccg acttgcgcgc cgcggcgccc ggcgcgggcc
      841 gcgcttcttt tttcggccgg agaaacaatc cccgggagcg cctcccggtt cgggccgtaa
      901 agcctggccg gcgtgctttt ccggcctgct aggattgcgg cgccaacgcc ggctgcttct
      961 ccggaaccaa ttgctttgtg gggtcgatgc gaagccaaat caaagcgctg gcgagagaga
     1021 gagcggccat gccggctaag ggaaggttcc agccgtgctt ttccaccagg ctgccgtaag
     1081 cggcggcggc gatcgtggat cccatttgcc ccgccatatt catcgcgccg cttaccgtgc
     1141 ccgcgtagcg cttgccgata tccaggcaga cagcccagca gttcggcagc atgaaatcgg
     1201 aaaccgcgaa gcttaaggaa agggcgataa tcgccagcag cttgttttga aggatgatcg
     1261 agagcagcat gaagccgccg gcgctggcca tgctcaccag gcctaccgac cggcggcccc
     1321 atttcaaccc gaccttgcgg accatgaaat cgctggtgta gcccccagcc acgttggccg
     1381 ccgctcccat caggtatggc agagccgcat acgtggcgaa cgcttcgatg cccttcgcct
     1441 tcaccagata cgtgggcatc caggaaaaaa accagtaggc ggcgtagcag tatgtgtgat
     1501 acataagcat cacggcccaa aggttggcgc tgctcagcgc cactttccag gagagcgatg
     1561 cgtgaggcgc cgggccgctc tcgccgatct ccagcagttc cggcgcgctc actcccgctt
     1621 tttgccccgg atagtcgcgg aaccacagat accagggaat cgcccaaagc aaccctgtgg
     1681 ccccgaagag aaaaaacacg ggacgccagc cgagccatgc ctgaagaggc aaggcgatgg
     1741 ccggggtgag cgctgccccg agccggctcg cggtccagac gatgccttgc gcccgggctc
     1801 gctctccgaa cgggaaccac cgggaaatgg cgcaggagct attggggaaa aaaccggcct
     1861 cgcccgcgcc aaatagaaag cggcaggcga gcaggctgaa atagctgcgg accgcgcccg
     1921 tcgcggccgt aaacgccgac caccacagga cgacacgggt caaaacccgc cgagggccga
     1981 aacggtctcc cagccagccg ctgggtatct cgaatatgcc gtacgcaaaa acgaacgcgc
     2041 tgaagacata acccatctgt gccgtggtca accccaggtc ttctttcatg tgcggcgccg
     2101 cagccgcaat actgatccgg tccagatagg tgacgatgga agccaggaac agcattccca
     2161 gcactttgtg acgtgcccgg atggcggcca ttcattcctt ctttattcgg ttccggggcc
     2221 ggtttccttg ccgccggcgt actttctgta gagcgcttcg attcccgctt cctgggcgtc
     2281 gccgggatgg atcaccacgc ggtagcggaa tcgaagcgac tggcccggcg ccagggtcac
     2341 gctgccgttg cggcccttgt cgttgtaaaa atcatgctcg ccaaaaatgt ttgccgcaaa
     2401 aaggccgtag ctccgggagt gccagtaggt cggatgcttc gggttgccgg gatgatcgaa
     2461 gatggcgatc cccagctttt ccccatccag ggttccagcg taatccaccc acttggagcg
     2521 tttcccccag acattctttt cgccctgctt gccctcggcc gtggtcatca ggccggtgcc
     2581 gggcgtaatt ccgggctgcg gcttcgcggc gggctcacag agcggctggg ccaggcgaat
     2641 ggcgaacgtc ccttccttcg tgtcgccgaa cacaaccttc tggtctgctt tcaaggtgat
     2701 gtcgaaatcg aggatccgca atttcggatc gccgtagatg attgttttgc gcgtttccgt
     2761 gatcagcgtc tgaccgctct gatccttcca atcaaacatc accaccatgc ttccggcctt
     2821 tttgccgctc ttcacctcga caaccttttc cagggatatg cgccccggtt taaactccga
     2881 ggcccccgga gtttccgggg cccagaagtc caggccgttg acctcgccat gggcgaacca
     2941 caagccgcga tggtgcgggt gatcgtgcgc ttcgcccgga gcttccgcga tgggccattg
     3001 gcgggtcacg atcttgccat ccgcggcccg caggggccac atataaggct ttagcgtgtt
     3061 ggggccgaaa aagagcgtcg agaagggctt gccgtcgatg gtgatgtcaa tctgcgtatt
     3121 gtcctggcgc gttagggtca cctgagcggt caggggcagc agaacaaaac aagctatcgt
     3181 aagcagaagc acgcattttc tcatggccaa aagtttggca cattgaaagc gcaaatgcca
     3241 gtgcgggatt gcggataccc ggcgcgaatc tccaatgcct gacgccgccg cactctgcga
     3301 ggcgtaccgg gatggtccac ccagccgggc tggcatgatg ccgggcctag ggcttcttcg
     3361 ctgtggccgc tttcgccttt tcgcgtccgg caacttcctt cgcgcgaaac ttcgcgatcc
     3421 gtccgatcaa cttcacggga accggccgta aaagcgggaa gcgaattgtg cccttgctca
     3481 tcttgtaccg ggcaagctcg tccttgaacg ccgcggcgag gcccccggtt gcggggtgga
     3541 gcgagtagtg cttcttccat ccggcaaaat agatcacaag gcgactatgc agccggtacg
     3601 cgggaatctg gtacgaaatc acttcgtcgg ctcccggcaa agccttgcgg atggcgctcc
     3661 gcacctgccc gagtaggtct cgcacggctt ccgggcgcga agcgatgtat tcgtccacgg
     3721 actggaagtt aggtctggcc atcgtgattc cttggattgt ctcacggcga aactgaaaat
     3781 aacggatcgt tgccgctttc tccgcgctcc ggcatccgcc ggtgaagtgg ccggcaaatg
     3841 atggatgcta ctctatagcc tagagcgctt gtgcgccacg gagagtgtga ttgtcctttg
     3901 agaacgaact gctcgagcag cgctaccaaa agatccggca actggaagag ctcggtttca
     3961 ccgcgtaccc acgaaagttc gcctacaccc acacccttgg cgaaatttgg aaacagtatg
     4021 gctccgcttc cgccgaagat ctggcggcga agcgggtcgg cgtgcgggtg gccggcaggg
     4081 tgctcaccgt ccggcgccag ggaaaggcgg gattcgccca catccagcag gggggagaca
     4141 agctccagat ctacgtgcgg caggacgcgg tcggtgaaaa cagcttccac ctttacaaaa
     4201 tgctcgatgc cggcgacatc atcggggccg agggctacct gtttcgcacc aaaaccaatg
     4261 agttgaccgt acacgtcgaa cgcctcgagt ttctctccaa ggcgctgctg gcgatgcccg
     4321 agaagtggca cggcctgcat gatgtcgaaa cgcgataccg ccagcgttac ctcgatctga
     4381 tcgccaatcc cgaggtgcgg gacgtcttcg tgcgccgcgc caaaattatt ggagcgctcc
     4441 ggcgcaccct ggaagcgcac ggctacattg aagtcgagac gcccatgatg cagccgctct
     4501 acggcggcgc gatcgcgaag ccgttccgca cccatcacaa tacgctcgat atcgacctgt
     4561 atctccgcat cgcgccggaa ctgtatttga aacggctggt ggtgggcggt ctggaccgcg
     4621 tttttgaaat caaccgcaac ttccggaatg agggcgtctc cacgcagcac aatcccgaat
     4681 tcacgatgct cgagttctat caagcctaca gcgactaccg cgacttgatg gatttcagcg
     4741 aagttctgct ccgggaagca gccatggccg ccctcgacgg caagaccgcg gcggccttcg
     4801 gcgagcatct catcgacttc agccgcctgg cgcgcttttc tatgaaggag gcggttgtgg
     4861 aattttggcc cgatccgggt acgcgcccgg ctatctccga cttgagcgac tgcgccagag
     4921 tggtggagct gatggaagcc cacaaccgca ccgttgagga gaaaatcgtg gcgccgcctg
     4981 gcgcaacggc gggggagttg gtcggcctgc tgtttgaaca ggtctgcgaa aagcggctca
     5041 ttcagcctac gatcatttac gactttccgg tcgaagtctc gccgctgtcg aaaaacaagc
     5101 cggacgaccc ggagtgggtg gagcgcttcg agatttacgc cgcaggcatg gaaattgcca
     5161 atgcgtactc cgagctgaat gacccgcagg agcagcggcg gcgtttcgag atgcaggccg
     5221 ccctgcggga gcgcggcgac gccgaaaccc acgagatgga tgaagactac atccgcgccc
     5281 tttgctacgc catgccgcca acggccgggg aaggcatcgg catcgaccgt ctgaccatga
     5341 ttctgaccaa ttcgcacagc atccgggacg tgatcctgtt tccgctgctg aggcccgagg
     5401 gcgacatcgg catcgccgcg caattgcgta aactggagga agcaagcgcg gatgggcgcc
     5461 agcgatgacc ggctcgaaca accgcccggg tcagggaaga ggccggagct gccggccccc
     5521 cgtacgcgcc cgtacatctg ctatccgctt ttcttagatg gctgtcgagt tttttatcgc
     5581 ccgccgttac ctgcgcgcca agcgcaaaca ggcggtcatt tccgtcatca ccgctatttc
     5641 cacaatcggt gtagcggccg gtgtgatggc gttgatcgtg gcgctggcaa tcaataatgg
     5701 attcggcgaa gaactgcgga atcatctgct gggcgcgacc tcccacgtca acctgatgga
     5761 gatcgagcgc ggcaacggca ttgccgattg gcgccggttc ctggaccgct tccgcaaggt
     5821 cccccacgtc gtggcgatcg ctccggcgct ctatgggcag gtgatgctct ccggcccaat
     5881 ccaggccaag ggagctgttc tgaagggcat cgatccgtcg tcggaactag ggatcggcga
     5941 tctgctcagg agcatcaagc aaggttccat cgacgccatc ctcgaagaaa aaggactgcc
     6001 cggtctgatc gtcggcaggc ggctcgctga cgaaatcggc ctgcgcatgg gaacggtggt
     6061 gaccatcgtc agcccgcagg gcgaactgac tccctttggg ccggcgccca gctacaaacg
     6121 gttccgcgta gtggcgatct ttgattcggg cttttacgat ttcgactcgc tttggacttt
     6181 taccagcctc aaagccgcgc agcaggcgct ttccctgggc gacgtaatca acgcggtgga
     6241 attcaagctc gacgatctgt accgggccga agaggtcagt aaagaaatag aaaaaatcgc
     6301 cggccctcgc ttctcaacga ccacgtggat ggagcagaac cgggcgctgc tgcacgcatt
     6361 gaaaatggag cgcctggtaa caatcgtcac gatcggcctg attgaaatgg tggcggctct
     6421 gaacatcctg atttcgctgg tcatgatggt catggaaaaa tatcgagaca tcgcgattct
     6481 catgtccatg ggcatgcggc gggagcaagt gcggaaaatt tttatgcttc agggtacgat
     6541 gatcggagcg ctcggcacag ccataggcct gacgctcggc tacacgctga gctggctggg
     6601 acagcgctac cattggatac accttgattc agatatttac ggcctcagct acgtcccgtt
     6661 tcaaccgcac ctctacgacg gcgcatgggt cgctgcggcg gccatgctgg tgagctttct
     6721 cgcgacgatt taccccgccc gcagcgccac ccggatcgcc ccggtcgaag ttttgagata
     6781 cgaatgaaac ccgctgttcc gtctttgcgg gttgcgtttc atctgatttg aatttgggga
     6841 ccgagaaagc cggacctcga ccctgcccct atctgctcag cctcgccagc caagcttgca
     6901 tgatcggcct ggcggctgtc ggaatccggt gcccgccgtc gagcatgtga aactccagat
     6961 ccgggatgcg cgtgcggaga cgctctggaa acgtctcagc cacaaccggc gggtaatact
     7021 cgtcctcgcg cgccgcgatg tgcaggacgg cggccggcga gtgctggtaa ggacgctggt
     7081 cccaatctcc cggtatgccg ccgcaaatcg cgattgcccc gcgcacggcg cccggatgcg
     7141 tgcaaagaaa gcggtaattc agggccaccg actgagaaaa acccaccagc acaacacgct
     7201 cggaggccgc accgatctcg gcgatcacat tgagaatcat atcgtgatgc agccgccacg
     7261 agtgctccgg ctcgaagcgc gtagaccaat gaaaggccac atccgccctc ggttcgccgt
     7321 tccgattcac ccagatttga tagggaccct ggagcgaagc cgcatagtgc tcctcgccga
     7381 cgagcggaac cgtgagccat agcatttgct ctggggtcat cgcgtggccg tgcagcgcca
     7441 caatgaaaaa cgcgcccggc gaggggtgtt ccggcttccg caagaggtaa ttgcatcgga
     7501 atggcagcag gaagcttcgt ctttcgatct gatcagaatt catcagcctg taatggatat
     7561 tgcgtaaaat aaaccgggcc cgcgcccgcc acgcggcgcc cgccccacaa tccggagcct
     7621 cttctttccg gtgacgatca ccccggctcg cctggcgcga gggcccgcgc caccgggcgc
     7681 ggatcgcctg cgcgcagcgg ccattcctgt caagccccgc ctgggattct cttttgcttc
     7741 cggatcctta cggtctggct tccaggacaa tgttaaacgg cgtttccgca gcgcgccgga
     7801 accgggtgaa gccgccggag gtcaccacct cgcgcagccg cgcctcgccg gcctgggctc
     7861 ccagcgccag gcccacctct tgggccagag acgccggagt gcagatcatt gtagatgcgg
     7921 cgtaatacac gcggcccaca gggttgagat tctcctccag ccggtcatgg gcgaagggct
     7981 caacgatcat ccaggcgccg tcgggcgcca ggctcttcaa cacgtgggcg gccgctccca
     8041 ctgggtcgcc catatcgtgc aggcaatcaa agaacgctac caggtcgtaa cccgcccccg
     8101 gataatcctt cgatcttgcg acttcaaacg tgactcgatc ggagacaccc gcctcctgcg
     8161 ccttcttacg ggcccgctcg attgagggag cgtgataatc aaaaccgacg aatttcgatt
     8221 tcggatagga ttgtgccatc aggatggtcg agctcccgtg gccgcagccg acgtcggcca
     8281 cccgcgctcc ggctttgagc ttgttttcca caccgtccag ggcggggatc caggagctca
     8341 ccaggtttgc cgcatagttc ggccggaaga agcgctccgt tccttcgaac aaacccggat
     8401 cgtgctcatg ccagcccagg cctgcgccag tgcggaaggc ttccgtaact ttcggttcgt
     8461 ccttcagagc cgacgcaacg agctggaacg cgccgggtag gtaggccggg ctgtcttcct
     8521 gcgccagggc aaaagcct
//
LOCUS       DASREK010000116        12333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1384954, whole genome shotgun sequence.
ACCESSION   DASREK010000116 DASREK010000000
VERSION     DASREK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12333
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384954"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFA54_05660"
     CDS             <1..935
                     /locus_tag="VFA54_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50325.1"
                     /translation="HEVPLRILPPNPSLQALPLRANLGEARQKITLRGSGLDRLERIE
                     SEGASIQLEPPRNPEMREAEIQLRPDVKKGASLGLLLYVEGLHSPLRAPDAVTVVGPR
                     PCIARVERSLPENLGVALKPGELPAGSVSSFSMRVKNLDSRPVLRFECIEPAKTLSAE
                     RLHPGESRPGARLEAAGSGTLFASVDPGAIGQAGCTLVAVVKTADGISDPYTLGQVVR
                     LPRIESFTLTEEKLGDSGYAGVLKGENLETIEKTGWDAQTGLPVSDLPKPVLGEGQKQ
                     MLRIPVPWPAPSPHAPLYIWLQGETQGRQTGAKY"
     gene            complement(1153..2025)
                     /locus_tag="VFA54_05665"
     CDS             complement(1153..2025)
                     /locus_tag="VFA54_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50326.1"
                     /translation="MITSVSRGPLGVLLVALSVAAAPAGAGVRSIDEIDHAFARFYNF
                     DFAGAHAVLNAYIAAHPDDPLAYAVRSAVYLYTEFDRLGILESEFFSDDAKITDKRKV
                     KPDPENRRLFFGAVEDARDRIRSRLAAKPEDYHAMFAACMVEGLLGDYTALVEKKQLG
                     SLSSDKRSNTCAQNLLKSYPDAYDAYVTTGFTEYLVGALPFYVRWFVHFDEVKGSKEQ
                     GIANLLVAARSGHYLRSFAKVLLAVVYLREKRLGQAREMLAELVRDYPENRVFRTELI
                     KISSRAQAAGTGTP"
     gene            complement(2199..3701)
                     /locus_tag="VFA54_05670"
     CDS             complement(2199..3701)
                     /locus_tag="VFA54_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZS50327.1"
                     /translation="MANAFRGRRGVAALFLVLALVIGGLLGSFLTTLLFGSGRSVPLL
                     VASAGNRGIGAVSFDSGFAEVAKAVLPSVVNVSSSKVVKVPGIGPESPFLDPFFRQFF
                     GNDFMRQFNVPREQREHSLGSGVIVSPDGYILTNYHVVEKATEIKVSRSGQREFKAKI
                     IGTDPKTDIAVLKVDEKNLPAITFGDSSKVRVGEFALAIGNPFGVGQTVTMGIVSATG
                     RGGLGIEAYEDFIQTDASINPGNSGGALVNVRGELIGINTAILSRSGGNNGIGFAIPV
                     NMARQVMEQILKHGKVVRGWLGVVIQPVTPEIAKAFGLSGEPRGALVSDVTPDSPAAK
                     AGLQKGDIIIDLNGQPVADSRELSLKVANLQPGSTAKLTLWRNGAQRELAVTLGEMPA
                     NLGRPGEGGGEGGGGTALQGLSVDELTPQIARQLGISPGTKGVVITDVQEGSAAAEAG
                     LRQGDVIQEVNRRPVTNVSEFQRAVRQAGREPVLLLVNRGGDTLYVVVEQ"
     gene            complement(4004..4303)
                     /locus_tag="VFA54_05675"
     CDS             complement(4004..4303)
                     /locus_tag="VFA54_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZS50328.1"
                     /translation="MTHIVPARTYVLVFAALLALAIGTTEIARIDLGPFNAVLAVTIA
                     VIKALLVALFFMHLLYTRHRTKIIAAAGVVWLGILIALTLSDTLTRNWLPVPRPW"
     gene            complement(4300..4953)
                     /locus_tag="VFA54_05680"
     CDS             complement(4300..4953)
                     /locus_tag="VFA54_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZS50329.1"
                     /translation="MQAAEAHEAVAHQFDDMEQQHDAATLGMWIFLVTEIMFFGGLFT
                     GYTVYRSAFPAAFAAGSRLCEVLLGGTNTGVLLLSSLTMALAVRSAQLDRRTHLIFFL
                     ICTMLLGLAFLSIKGLEYFHKYQEHLIPGSHFAFAGPDPRQVQLFVYFYFAMTGLHAF
                     HMIIGVGVVLVMLLLAFQGKLTSRRYDPVEVAGLYWHFVDIVWIFLFPLLYLVDLHK"
     gene            complement(4955..6583)
                     /gene="ctaD"
                     /locus_tag="VFA54_05685"
     CDS             complement(4955..6583)
                     /gene="ctaD"
                     /locus_tag="VFA54_05685"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HZS50330.1"
                     /translation="MSTMPAELEIHTHEHYLNASYGIRSWLLTTDHKRIALLYLASIT
                     FMFFVGGAFAVLMRLNLLTPEGEMVSAETYNKLFTMHGLIMIFFFLIPSIPAVLGNFV
                     IPLMIGARDVAFPKLNLLSWYLFITGSILALYSAIAGGVDTGWTFYTPFSTAYSNTHV
                     LSMAAGVFITGFSSILTGLNFVVTIHTMRAPGLTWYRLPLFLWSLYATSVIFILGTPV
                     LAIALLLLGLERLFGVGIFNPSLGGDPILFQHLFWFYSHPAVYVMVLPAMGVISEVVP
                     CFSRKRIFGYRIIAFSSMAIAIIGFLVWGHHMFVSGQSIYAGLIFSFLSYLVAIPSAI
                     KVFNWTATLYKGAISYATPMLYAFGFIGLFTMGGLTGLFLAALAVDVHVTDTYFVVAH
                     FHYIMVGGAVMAYLGGIHYWWPKISGRLYPEGWAKLSALVVFLGFNLTFFPQFILGYL
                     GMPRRYHSYPAEFQVLNVMSTAGASILAVGYLMPLVYLIWSMRYGPLAPANPWQATGL
                     EWQTPSPPPEHNFEQTPIVTRAPYGYDEMEEQRV"
     gene            complement(6580..7524)
                     /gene="coxB"
                     /locus_tag="VFA54_05690"
     CDS             complement(6580..7524)
                     /gene="coxB"
                     /locus_tag="VFA54_05690"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZS50331.1"
                     /translation="MQQLQLFPNRASTIGTGVDHLYFYLVGVSGFFSILIFTLIFIFA
                     IKYRRRSPADRAQQIAGNVPLELAWALIPFGLEMIMFFWGASLYFRHYQAPPDAEDIY
                     VVGKQWMWKLQHPEGPREINELHVPVGRPFRLILASEDVIHSFYIPAFRIKQDAVPGM
                     YTTEWFQATSVGKYHLFCAEYCGTNHSRMTGWIYVMDPGDYQNWLSGGAPGESMAAAG
                     ARHFSRLGCAGCHVPDNSGRGPSLVGLYGSQVRLVGGRTAFADDSYIRACIMTPSVNR
                     VANYPPIMPTFRGVISEEELSQIVEYIKSLNQGARIQP"
     gene            complement(7527..8372)
                     /locus_tag="VFA54_05695"
     CDS             complement(7527..8372)
                     /locus_tag="VFA54_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZS50332.1"
                     /translation="MIRLLLAAAGLAVFAPAFAQTGLSPAPAPAGFNAAAVQTGLPPA
                     LRNVGIDQKLNAQAPLDIPFRDEAGKTVQLRQYFGRQPVILALVYYDCPMLCTLVLNG
                     LVRSLRAISLNPDQHFQIVTVSFDPRETPALAMAKKQQYINNYRRPGAEQGWHFLTGE
                     EASIEKLTRAVGFRYAFDAKSQQFAHASAIMVLTPEGRLARYFYGLEYSARDLRLALI
                     EASANKIGTPADQVLLFCFHYDPATAKYSLAIMNALRAAGAATALALGGFLFVMFRRD
                     RRGSR"
     gene            complement(8369..8956)
                     /locus_tag="VFA54_05700"
     CDS             complement(8369..8956)
                     /locus_tag="VFA54_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50333.1"
                     /translation="MPEHGAEDERGNSENRNQPASLWDVLRSGAGLAVMTLLNLLGGG
                     SRAERRPAAPKVLAEEENKPAPAQAAAGHERRDVNVKGLLAFGAVLCVVIALTALGMD
                     RLFRHYASSQPAGLSAREAAAPELPPEPRLEIDMHADLDRLRAHEDQVLHSYGWVDRR
                     NGTVRIPIGRAMELILRQGLPVWQSAPAARKGNDE"
     gene            complement(8956..10152)
                     /locus_tag="VFA54_05705"
     CDS             complement(8956..10152)
                     /locus_tag="VFA54_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50334.1"
                     /translation="MNGAGPADFRLQRLEQRCFAIGVLALAACIAAAFRSPDQFFRSY
                     LMAYVFWSGVCIGCFPVLMMHHLTGGGWGYLIRRLLEAALRALPVMAALVLPALLGIP
                     HLYTWAHPQAMAGNAALRHKRIYLNVPFFALRTVFYFAIWALLAHYLNKWSGEQDRAD
                     APETRRRLYNLSGPGLVIYGLTMTFASMDWVMSLEPEWFSTIYSAMFMVGQVLSSLAF
                     AIAVLLLFIRQAPLREIAAPKYLNDLGNMLLTFVILWAYLAFSQFLIIWSGNLTDEIP
                     WYLHRSRQGWQWIAMALFVFHFAIPFLVLLSRDIKRQAEALSALAGALFLVRLLDMFW
                     MVNPAFDTAGVRFHWLDWAATLGIGGIWVGAFFRRLGQKPLLPVHDPELQAAIERIGK
                     ARVGEA"
     gene            complement(10149..10742)
                     /locus_tag="VFA54_05710"
     CDS             complement(10149..10742)
                     /locus_tag="VFA54_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZS50335.1"
                     /translation="MSRPARHKKIAAGWICAWLAAIAPGCRQVMNEEPRYNALAESDF
                     FPDRQAARPLVEGTVARGHLHTDRAFYSGMSGDAPVARIPFPVTRQVLERGQERFNIF
                     CSPCHGRLGDGQGMIVKRGLRGPPSYHIARLREAPDGHFFDVITNGYGAMYSYASRVP
                     PRDRWAIIAYIRALQLSQAAPLAAVPPEERRKLEAQP"
     gene            complement(10739..11275)
                     /locus_tag="VFA54_05715"
     CDS             complement(10739..11275)
                     /locus_tag="VFA54_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HZS50336.1"
                     /translation="MNKPRIYGLIAEFDRPEDLRAAARRAYEEGYRRLDAFSPLPIEG
                     LADAMGLHRTQVPLIVLAGGLVGCLGGFYLQYWIHAIDYPINVGGRPYNSWPSFIPVT
                     FEMTVLFAALAALLGMLALNGLPMPHHPVFNVPRFALATRDRFFLCIEARDPKFDPEA
                     TRRFLESLHAREVSEVPR"
     gene            complement(11279..>12333)
                     /gene="nrfD"
                     /locus_tag="VFA54_05720"
     CDS             complement(11279..>12333)
                     /gene="nrfD"
                     /locus_tag="VFA54_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZS50337.1"
                     /translation="QEWRTSINRFAEAMTLFAVACAGMFPLLHLGRPWFFYWLFPYPS
                     TMGVWPQFRSPLVWDVFAVSTYATVSFLFWFVGLIPDLATLRDRARHWLPRKFYGFLA
                     MGWRGSAFHWRRYQVAYLLLAGLATPLVVSVHSVVSFDFTIAILPGWHSTIFPPYFVA
                     GAIYSGFAMVMTLAIPLRAIYGLEDFITLRHLDNMAKVMLATGLIVAYGYMMETFIAW
                     YSGNQFEQFLIHNRMFGPYAKVFWAVILCNVAAPQLLWSPRVRSNVAALFAIALAVNL
                     GMWLERYMIVVVSLHRDFMPSAWGMYAGTRWDYATFAGSIGLFLALMFLFIRFLPVIS
                     IFELRELVHRPAPHSG"
BASE COUNT         2642 a         3955 c         3624 g         2112 t
ORIGIN      
        1 tacacgaggt cccccttcgg atcctgccgc ccaacccgtc gctccaggcc ctgcctttgc
       61 gggcgaatct cggcgaggcg cgccagaaga ttacactccg gggttcgggt ctcgaccgtc
      121 tcgagcgcat cgaatccgaa ggggcgtcca tccaactcga gccgccgcga aacccggaga
      181 tgcgcgaggc ggagattcaa ctgcgtccgg acgtcaagaa gggcgcgagc ctcggtttgc
      241 ttctctacgt cgaaggactc cacagtcctt tgcgggcgcc ggatgccgtc acggtggtcg
      301 gtccaaggcc gtgcatcgcc cgcgtcgaac gctctttgcc tgagaattta ggcgtcgctc
      361 tcaagcctgg cgaactgcct gccggctcgg tttcgagttt ttccatgcgc gtgaaaaatc
      421 tggattcgcg gcccgtgctc cgcttcgagt gtatagagcc cgccaagacc ctatcagcgg
      481 aaaggctgca tccgggcgaa agccgtcccg gcgcgcggct ggaagctgcg ggaagcggga
      541 ccctgtttgc atcggtggat cccggcgcga ttggtcaagc cggctgtacg ctggtggcgg
      601 tggtcaaaac ggcggacggc atctccgatc cttacacact cggccaggtt gtgcggctgc
      661 cgagaattga aagtttcaca ctgaccgagg aaaagctggg cgattccggc tatgccgggg
      721 tcctcaaggg cgagaacctg gaaaccattg agaagacggg ctgggacgcg caaaccggct
      781 tgccggtcag cgaccttccg aagccggtgc tcggtgaagg acaaaagcaa atgctccgca
      841 tccccgtgcc ctggccagcc ccatcgcccc acgctcctct ttatatctgg ctccagggcg
      901 aaacccaggg ccgccagacg ggcgcgaagt attgattgaa tttcgccggc gagtcgcggg
      961 agctcagttc cggcaagcgg gaaagtccag ccatcggcgg caactcggtg cgcccgccgc
     1021 gacagtgtga tcgctcccgc cgcggtgcct ggctttgtct ccggcgcgcg gctggctccg
     1081 cccggcatcc cgttccgtta tctcgagcct tggggagagg gatctgcctt ttttcaaccg
     1141 ccgatcgggt tcttagggcg tgccggtccc ggctgcttgc gcccggctgg aaatttttat
     1201 gagttccgta cggaaaacgc gattctctgg atagtcacgg acgagttccg ccagcatctc
     1261 cctggcttgg ccgagcctct tttccctcag gtatacgacg gccaataaga ccttggcgaa
     1321 ggaccgcagg taatggccgg agcgcgccgc caccaggaga ttcgcaatcc cttgctcttt
     1381 gctgcctttc acctcatcga aatgaacgaa ccagcggacg taaaaaggaa gcgctcccac
     1441 caggtattcg gtgaagcccg tcgtgacgta ggcatcgtag gcgtccggat agctcttcaa
     1501 aagattctgc gcgcaggtgt ttgaccgctt gtcggaagaa aggctcccca gttgcttttt
     1561 ctcgaccagc gccgtgtaat cgcccaacag cccttccacc atgcacgcgg cgaacatcgc
     1621 atggtaatcc tcgggtttgg ccgccagccg ggaccggatt ctatcgcgcg cgtcctctac
     1681 tgctccaaaa aacagccgcc ggttttcggg atccggcttc accttccttt tgtccgtaat
     1741 cttcgcatcg tccgaaaaaa actccgactc cagaatgccg agccggtcga actccgtata
     1801 caaatacacg gcggaacgga ccgcgtaagc cagcgggtcg tcgggatggg cggcaatgta
     1861 agcattcaag acggcgtggg cgccggcaaa atcaaaattg tagaaacgcg caaaagcgtg
     1921 atcgatttcg tcgattgacc ggacgccagc ccctgcgggc gcggccgcca cgctcagcgc
     1981 caccagcaat acgcccaggg ggcctcgaga cacgctggta atcatagcag ttaatccgtc
     2041 cctgaggcgt gtgcgattga gcgggaggaa gtgctttctt ttctccttcg caccaccccc
     2101 tgtctccggc gccgggcaga ccatcgaaag ctgggggcgc ccgggggaag acatggcgtg
     2161 agcgggcggc ttcgcccgct cacgcgccgg tgcgcctgct actgttccac cacaacgtat
     2221 aaagtatcgc cgccccggtt cacgagcagc aggacaggtt cccggccggc ctgccgcacg
     2281 gcccgctgga actcggaaac gttggtcacg ggcctgcgat tgacctcctg gatcacatcg
     2341 ccttgccgga ggcccgcttc cgccgcggcg ctgccctcct ggacgtcggt aatcacaaca
     2401 cccttggttc ccggagagat accaagttgg cgggcgatct gcggcgttag ctcatccacc
     2461 gataggcctt gcagagctgt gccgccgcca ccctcgccgc cgccctcgcc aggacgcccc
     2521 aggttggccg gcatctcgcc cagggttacg gcaagttccc tctgcgcgcc gttccgccac
     2581 agcgtcagct tggctgtgga acccggctgg agattggcga ccttcaggct cagctcgcgg
     2641 ctgtcagcca ccggctgtcc gttcagatcg atgatgatgt cccccttctg taaaccggcc
     2701 ttggccgccg ggctgtccgg cgttacgtcg ctcaccagcg cgccgcgagg ctcgcccgaa
     2761 agcccgaagg ctttcgcgat ctccggagtg accggttgga taaccacgcc gagccagccg
     2821 cgcaccactt tcccatgctt gaggatctgc tccattacct ggcgcgccat gttgacggga
     2881 atcgcgaagc cgatgccgtt attgccgccg ctgcgcgata ggatagccgt gttgatgccg
     2941 atcagttccc ctcgcacgtt taccagcgcg ccaccggaat ttccggggtt gatcgaagcg
     3001 tcggtttgaa taaaatcctc gtaggcctca atgccgagac cgccgcgccc cgtggcgctc
     3061 acaatgccca tcgtcaccgt ctggccgacg ccgaacggat tcccaatcgc cagcgcaaac
     3121 tcgccgacac ggaccttcga ggaatccccg aacgtgatcg ccggcaaatt cttctcgtcc
     3181 accttaagga cggcgatgtc ggttttggga tcggtcccga tgatctttgc cttgaattcg
     3241 cgctgcccgc tgcgcgatac cttgatctcg gtcgccttct ccaccacatg atagttcgtc
     3301 aggatgtagc cgtccggact gacgatcacg ccggatccca gactgtgctc ccgctgctcc
     3361 cgcgggacgt tgaactggcg catgaaatcg tttccaaaga attggcggaa gaacggatcg
     3421 agaaacggcg actccggacc gatgcccggc actttcacca ccttcgaaga cgaaacgttc
     3481 accaccgagg gaagcaccgc cttcgccacc tcagcgaaac cggagtcgaa cgaaaccgcc
     3541 ccgattccac gattgccggc gctggcaacc agaagcggca cgctccgtcc cgagccaaac
     3601 agtaaagttg tcagaaatga gcctagcagg ccgccgatca ccagggccaa aacgaggaaa
     3661 agcgccgcca cgccgcggcg gccccgaaat gcgttagcca taaagatcct cctgcgagaa
     3721 attccggaca accggaccgg gagccctgcc cctcacggat ctagcgcaag agccgggcgg
     3781 gctgcgaaag attccataat atagacggcc ctgagagatc ggaggtttac aggagaacag
     3841 ccgaacaaga cgctggaggt tgcgcttagc gagtctcatt gacagactcc cagcccgaac
     3901 ctgcctccca tcatacaacc gccggatcag cccggaaaaa ggtctgtctt ctgctacacc
     3961 ccgtggccat cctcgcaagt agacaagcgt ctggcgcccc cctctaccac ggtctgggaa
     4021 ccgggagcca gtttcttgta agggtgtcgc tcagcgtcag cgctatcaga atgccgagcc
     4081 ataccacgcc tgccgccgcg atgattttcg tccggtggcg ggtgtagagc agatgcataa
     4141 aaaataacgc caccagcaag gccttgatga cggcgatcgt gaccgccagc actgcattga
     4201 acggtccgag atcaatccgg gcgatctccg tggtgccgat ggccagcgcc agcagcgccg
     4261 cgaataccag aacataggtc ctcgctggaa cgatgtgagt catttgtgca agtccaccag
     4321 ataaagcagc ggaaaaagaa aaatccagac gatatcgacg aagtgccagt acaggccggc
     4381 cacttcgact ggatcatagc gccgcgaggt aagctttccc tgaaacgcga gcagcagcat
     4441 gaccagaacg acgccgacgc cgataatcat gtgaaacgca tgaaggccgg tcatggcgaa
     4501 atagaaatag acgaagagct gcacctggcg cgggtcgggt ccggcgaatg cgaagtggct
     4561 tcccggaatc aggtgctctt ggtatttgtg aaagtactcg agccccttga tgctcagaaa
     4621 cgccaggccc agcagcatgg tgcagatcaa aaagaaaatg agatgcgtcc gcctgtccag
     4681 ttgagccgag cgcaccgcca gagccatggt caagctgctc agcagcagca cgcccgtgtt
     4741 ggttcctccc agcagcacct cgcatagccg gctgccggcg gcgaacgccg ccgggaacgc
     4801 ggaccggtat acggtgtagc ccgtgaacag cccgccaaaa aacatgatct cggtgaccag
     4861 aaaaatccac atccccagcg tcgccgcgtc atgctgctgc tccatgtcgt cgaactgatg
     4921 cgccactgcc tcgtgcgcct cggcggcctg catttcagac acgctgctcc tccatctcgt
     4981 cgtagccgta gggggcccgc gtcacgatcg gcgtctgttc aaagttgtgt tccggcggcg
     5041 gcgagggagt ctgccactcg agccccgtcg cttgccacgg attcgccggc gccagcggtc
     5101 cataccgcat ggaccagatc aggtacacca ggggcatcaa gtaccctacc gcgagaatcg
     5161 aagcgcccgc ggtggacatg acgttaagca cttgaaactc ggcagggtag gaatgatatc
     5221 tacgcggcat gccgagatat ccgagaatga attgcggaaa gaacgtgaga ttgaatccga
     5281 gaaagacgac cagcgccgac aacttggccc atccttccgg gtacaatcgc ccgctgatct
     5341 tcggccacca gtaatgaatt ccccccaggt atgccatcac agcgccgccc accatgatgt
     5401 agtggaaatg ggcgactaca aaatacgtgt ctgtcacgtg aacgtccacc gccagcgcgg
     5461 ccaaaaaaag gcccgtcaac ccccccatcg tgaaaaggcc gatgaagccg aaggcgtaaa
     5521 gcatgggcgt ggcgtagctg atcgcgccct tatacagcgt ggcggtccag ttgaacacct
     5581 tgatcgccga aggtattgct acaagatagc tcaggaaaga aaaaatcagc ccggcgtata
     5641 tcgactgccc gctgacgaac atgtgatgtc cccacaccag aaagccgatg atcgcgatgg
     5701 ccatgctcga aaacgcgata attctgtatc cgaagatgcg ctttcgcgaa aagcagggga
     5761 caacctcgct gatcaccccc atggccggca gcaccatcac gtagaccgca gggtgcgagt
     5821 agaaccagaa caaatgctgg aacagaatcg ggtccccgcc cagcgaaggg ttgaaaatgc
     5881 caacgccgaa caacctttcc agccccagca gcagcagcgc gatagccaga accggcgtac
     5941 ccaggatgaa aatcacgctg gtggcgtaca gcgaccagag gaacaggggc agccggtacc
     6001 aggtcaggcc cggcgcgcgc atcgtgtgga tggtgaccac gaagttcagg ccggtcagga
     6061 tcgaggaaaa cccggtgatg aaaacgccgg ccgccatcga gagtacgtgc gtgttcgaat
     6121 aagccgtgct gaacggcgtg taaaacgtcc acccggtatc gacgccgccc gcaatggcgg
     6181 aatacagcgc cagaatgctg ccggtgatga acaggtacca gctcagcagg ttcaacttgg
     6241 ggaaagccac gtcccgcgcc ccgatcatca acggaatgac aaaatttccg agcacggccg
     6301 ggatggacgg aatcaggaaa aagaaaatca tgatcaggcc gtgcattgta aacagcttgt
     6361 tgtaagtttc cgccgatacc atctcccctt ccggcgtcag cagattcagc cgcatcaata
     6421 ccgcaaaggc gccgccgacg aaaaacatga aggtgatcga agccaaatac agcagcgcga
     6481 tgcgcttgtg gtcggtggtg agcagccacg accggatgcc gtagctggcg ttcaaatagt
     6541 gctcgtgagt gtgaatctcg agctcggcgg gcatcgtgct catggctgga tcctcgcgcc
     6601 ctggttgaga gacttgatgt attccacgat ctgcgacagc tcctcctcgc tgattacgcc
     6661 ccgaaaagtc ggcataatcg gcggataatt cgccacccgg ttgacgctcg gagtcatgat
     6721 gcaggcccga atgtagctgt catccgcgaa ggcggttctc ccgcctacca gccggacttg
     6781 tgacccatat aagcccacca gcgacgggcc gcgtccgctg ttgtccggca catggcaccc
     6841 ggcgcatcct agtcgggaaa aatgcctcgc tccggcggcc gccatcgatt cgcccggagc
     6901 gccgccgctc agccagtttt ggtaatcgcc cgggtccatc acgtagatcc agccggtcat
     6961 gcgggagtgg tttgtgccgc aatattccgc gcagaaaagg tgatacttgc cgacgctggt
     7021 cgcctgaaac cattccgtgg tgtacattcc cggaacggcg tcctgcttga tccggaacgc
     7081 cggaatgtaa aagctgtgaa tcacgtcctc ggaggccaaa atcagccgga acggccggcc
     7141 caccggcaca tgcagctcgt tgatctcccg cggaccttcc gggtgctgga gcttccacat
     7201 ccattgtttg ccgaccacat aaatgtcctc ggcgtcgggc ggcgcctggt agtgccggaa
     7261 gtacaggctc gccccccaaa agaacatgat catttccagg ccgaagggga tcagggccca
     7321 ggccagctcg agcggcacat tgcccgcgat ttgctgcgcc cggtccgccg gcgagcgcct
     7381 gcggtacttg attgcaaata taaaaatcag cgtaaaaatg agaatcgaaa agaaccccga
     7441 cacgccgacc aggtaaaaat aaagatggtc cacgcccgtt ccgatggtgg acgcccggtt
     7501 tggaaatagc tgcagttgct gcattctcaa cggctcccgc ggcggtcgcg ccggaacatc
     7561 acaaacagga agccgcccaa agccagcgcc gtggccgccc cggccgcgcg caaggcattc
     7621 attatcgcca ggctgtattt cgccgtcgcc gggtcataat ggaagcaaaa cagcagaacc
     7681 tgatcggcgg gagtgccgat tttgttcgcc gaagcttcga tgagggccag ccgcaggtcc
     7741 cgggcggaat actcgagccc gtaaaaataa cgggccaggc gcccttccgg agtcagcacc
     7801 atgatggcgc tggcgtgcgc aaattgctgc gacttcgcgt cgaacgcgta gcgaaacccc
     7861 accgccctgg tcaacttttc aatcgacgct tcctcgcccg tgagaaaatg ccagccttgc
     7921 tcggccccgg gccggcggta gttgtttata tattgctgct tcttagccat cgccagagcc
     7981 ggcgtttccc tcgggtcgaa gctcaccgtg acgatctgaa aatgctgatc ggggttaagt
     8041 gaaatggccc gcaggctcct caccagccca ttcagcacca gcgtgcagag catcgggcag
     8101 tcgtaataaa ccagcgccag gatcaccggc tggcggccga aatactgccg gagctgcacc
     8161 gttttccccg cctcgtcgcg aaacgggatg tcgagcggcg cttgcgcgtt cagcttctga
     8221 tcgatgccta cattccgcag agccggcggc agcccggtct gcacggcggc ggcgttgaag
     8281 cccgctggcg ccggcgcggg gctgaggccc gtttgggcga aagcgggtgc gaaaaccgcc
     8341 agcccggcgg ccgccaacag caatcggatc attcgtcgtt cccctttctt gcggccgggg
     8401 ccgattgcca gaccggaagg ccctgccgca aaatcagttc catggctcgg ccgatgggga
     8461 tcctcaccgt tccgtttctc cggtccaccc acccgtagct gtgcagcact tggtcttcgt
     8521 gcgcgcggag ccggtcgagg tctgcgtgca tgtcgatctc aagccgcggc tccggaggca
     8581 gttccggcgc ggcggcctcc cgcgccgaca gtccggcggg ctggctggaa gcgtagtggc
     8641 gaaacagccg gtccatcccc agagccgtca gcgcgatcac gacacacagg accgcgccga
     8701 acgccagcag ccctttgaca ttcacgtcgc ggcgctcatg cccggcggcc gcctgagccg
     8761 gagccggctt gttttcctct tccgcgagaa ccttcggggc cgccggccgc ctctcggcgc
     8821 ggctgccgcc ccccagcaga ttaagtagag tcatcaccgc caggcccgcg cccgagcgca
     8881 gcacatccca caggctcgcg ggctgattcc ggttctcgga attgcctcgc tcatcctctg
     8941 cgccgtgctc tggcatcaag cctctcctac acgcgcttta ccgatgcgtt cgattgccgc
     9001 ttgcaactcc ggatcatgga ccggcagcag cggcttttgc cccagccgcc ggaaaaaggc
     9061 gcccacccaa atgccgccga tccccaaggt cgcggcccaa tcgagccagt gaaaccgtac
     9121 tccggccgta tcgaaagccg gattgaccat ccaaaacata tcgagcagcc ggaccaggaa
     9181 cagcgccccg gccagcgctg aaagcgcctc ggcctgacgt tttatgtccc gggaaagcag
     9241 gacgagaaac gggatcgcga aatggaacac gaacagcgcc atcgcaatcc attgccatcc
     9301 ctgccgggag cggtgcaagt accatggaat ttcgtccgtc aagttgcccg accagataat
     9361 cagaaactgg gaaaacgcca gataggccca caagatcacg aacgtcagca gcatgttgcc
     9421 caaatcgttc agatatttcg gcgccgcaat ctcgcgcagc ggggcctgcc ggatgaacag
     9481 cagcagcaca gcgattgcga aagccaggct ggacagcacc tggcctacca taaacatcgc
     9541 gctgtaaatc gtcgaaaacc attcgggctc gagcgacatg acccagtcca tcgaagcgaa
     9601 cgtcatcgtc aagccgtaaa tcaccagccc ggggccgctc aggttgtaaa ggcgccgcct
     9661 ggtctccggc gcatcggcgc ggtcctgctc gcccgaccac ttgttgagat agtgcgccag
     9721 caaagcccag atcgcgaagt agaaaacggt gcgcagcgcg aaaaaaggca cgttcagata
     9781 gatccgcttg tgccgcaaag ccgcattccc ggccatcgcc tggggatgcg cccatgtata
     9841 taaatgagga atacccagaa gcgccgggag aacgagcgcg gccatcacgg gcaaggcccg
     9901 cagcgcggct tccagcagcc tccggattag gtagccccag ccgccgcccg tgaggtggtg
     9961 catcatgagt accggaaaac acccgatgca cacgccgctc cagaatacgt aagccatcag
    10021 ataggagcgg aaaaattggt ccggcgaccg gaatgccgcc gcgatgcacg cggcaagagc
    10081 cagaacgccg atggcaaagc agcgctgctc gaggcgctgc aaccggaaat ccgccggccc
    10141 cgctccgttc acggctgcgc ctccagtttc cggcgttctt ccggcggcac ggcggcgagc
    10201 ggcgccgcct ggctaagctg taaagcgcgg atatacgcga taatcgccca tcggtcccgc
    10261 ggcggaaccc gcgaggcgta gctgtacatg gctccatacc cattcgtgat tacatcgaaa
    10321 aagtgcccgt caggcgcttc ccgcagccgg gcgatgtgat aggaaggcgg cccgcgcaat
    10381 ccgcgcttca cgatcatgcc ctggccgtct cccagcctcc catggcaagg agagcagaag
    10441 atgttgaacc gctcctgccc gcgctcgagc acctgccgcg tgaccgggaa ggggattcgc
    10501 gccactggcg cgtccccgct catgccgctg tagaacgcgc gatcggtgtg aagatgcccg
    10561 cgcgccaccg tgccttccac cagggggcgc gcggcctgcc ggtctggaaa gaagtcgctc
    10621 tcggctaggg cgttgtacct tggctcttcg ttcatcacct gccggcagcc cggagcgatc
    10681 gcggccagcc aagcgcaaat ccagccggcg gcgattttct tgtgtcgcgc cggccggctc
    10741 accgcggcac ctcggaaact tcgcgggcgt gcaacgactc cagaaaccgc cgggttgctt
    10801 ctggatcaaa cttcggatcc cgcgcctcga tgcacaggaa aaagcggtcc cgagtcgcca
    10861 gcgcgaagcg cggcacgtta aataccggat ggtgcggcat gggcagcccg ttcagagcca
    10921 gcatgcccag cagcgctgcc aaggcggcga atagcacggt catttcgaaa gtcaccggta
    10981 tgaatgacgg ccagctattg tacggccggc ccccgacgtt aatgggatag tcgatggcat
    11041 gaatccaata ctggagataa aagccgccca ggcagccgac gaggccgccg gccagcacaa
    11101 tcagcggcac ctgcgtgcga tgcaacccca tcgcatcggc gagtccctcg attggaagcg
    11161 gcgaaaaagc gtccagcctg cgatagcctt cttcgtaggc tcgccgcgcg gcggcccgca
    11221 agtcctccgg cctgtcgaat tcagcaatca atccgtaaat gcggggctta ttcatcgctc
    11281 atcccgagtg cggcgcgggg cggtggacca gttcccgcag ctcgaaaatc gaaatcaccg
    11341 gcaggaatcg aatgaataga aacatcagcg ccagaaacag gccaatcgag ccggcgaaag
    11401 tcgcgtaatc ccaccgcgtc cccgcataca tcccccacgc cgagggcatg aagtcccgat
    11461 gcaggctcac caccacgatc atgtaccgtt ccagccacat gcccagattc acggccaggg
    11521 cgatcgcaaa aagggcggca acgttcgatc gtacgcgcgg cgaccatagc aactgaggcg
    11581 ccgccacgtt gcacagaatc accgcccaga aaaccttggc atagggcccg aacatccggt
    11641 tgtgaatcag gaactgctcg aactggttcc cgctgtacca ggcgatgaac gtctccatca
    11701 tgtagccgta ggcgacgatc agtcccgtgg ccagcatcac cttggccatg ttgtccaagt
    11761 ggcgcagggt gatgaaatcc tccaggccgt aaatcgcccg cagcgggatg gccagcgtca
    11821 tcaccatggc gaagcccgag tagatcgccc ccgctacgaa gtacggcggg aagatcgtgc
    11881 tgtgccagcc gggaagaatg gcgatggtga aatcaaagct gaccacgctg tgtaccgata
    11941 cgaccaacgg cgtggccagg ccggccagca gcagatacgc gacctggtag cgccgccagt
    12001 ggaaggccga tccccgccat cccatcgcca ggaatccata gaacttgcgc ggcagccaat
    12061 gccgcgcccg gtcgcgcagc gtggccagat cgggtatcag gcctacgaac cagaaaagga
    12121 aggaaacggt cgcgtaggtc gagaccgcga atacgtccca caccagcggg ctgcggaatt
    12181 gcggccatac ccccatggtg ctcggatacg ggaacagcca gtaaaaaaac cacggccgcc
    12241 ccaaatgcag aagaggaaac attccggcgc aggctacggc aaacagtgtc atcgcttcgg
    12301 cgaagcggtt aatcgacgtg cgccattcct ggt
//
LOCUS       DASREK010000117        11011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_394140, whole genome shotgun sequence.
ACCESSION   DASREK010000117 DASREK010000000
VERSION     DASREK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394140"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFA54_05725"
     CDS             <1..398
                     /locus_tag="VFA54_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HZS50338.1"
                     /translation="TNVIKDGEEVIGSRTKVKIVKNKVAAPFRSAEFDILYGEGISRE
                     GDLIDLGAEHNIIEKSGAWYSYKGERIGQGRENAKQFLRENQDIRADLDTELRRILGL
                     LPAVPNGSPPAEATPAAKAGANTKAAVRG"
     gene            complement(579..1601)
                     /locus_tag="VFA54_05730"
     CDS             complement(579..1601)
                     /locus_tag="VFA54_05730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS50339.1"
                     /translation="MRQTHTYHHLIPREYPGLLSIVIPVWNEQEAIPFLRMRLASFMN
                     ELPCPAELILVNDGSSDRTLDLLLDWAAADSRVKIVNLARQFGHQAASTAGLDHAAGD
                     AVVLMDADLQDPPEVILDMLAEYCRGFDVVYGRRTSRQGETIFKRATAWIFYRLMRAL
                     IYHDLPVDVGDFRLISRRCLDALIAMRETHRFLRGMVAWVGFAQTAVPYVRQPRVKGE
                     TKYPLVKMLRFAWTAAISFSPTPLRLSFALGVLLGVIGMAQGINAVVRHMLGMFTVPG
                     WTSLMVAICFIGGAILISIGILGEYIGRIFEEMKGRPIYVVASSANFPSSRAYLNPEK
                     KEAVHH"
     gene            complement(1620..3197)
                     /locus_tag="VFA54_05735"
     CDS             complement(1620..3197)
                     /locus_tag="VFA54_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50340.1"
                     /translation="MSTSVSIVAHREPGVLLSLAKRLLAPSYTDLFFIFVMTWSFLAG
                     ANGWQRLFMDGDTGLHIRIGDYILAAHQVPVRDLFSFSKPGQDWIAFEWLSELIFALL
                     HRSFGLKGVALLSGVVIGALITILLVYTLWRGAHSMIALGLVLMALNAINIHFHARPH
                     LFTLLFLTAGIWLIFADRRERSPAVWLLAPLTALWANMHGGFVLFLALLGLLVIGSAA
                     EAAWTGEPVVSRSSEAVRYAGVSLACAAASLVNPYGLRLHAHIFFDVIRSKWTMDLVD
                     EFKSPSFRSEQMMHFMALLFLGLIIVAPLLEKRRIVEALWIVFLAYCSLISVRHAPIY
                     MIVATPVIAVQLSDYWRRWSSTRGRRSIPGIFDDLAAQLRINFRFPSLLTLGFLVAIG
                     MSGWMHWPAGFSPEFFPVRMVSRHADALASSRVFTFDQWADYLIYRNYPRQRVFLDGR
                     HQYYGEKIVRDYLKLRSGDLAWKDLAAKYRFDLALCSPDTPLATLMKSDPQWRIVEDD
                     GVAILFVKKEEKPGLTP"
     gene            complement(3760..3933)
                     /locus_tag="VFA54_05740"
     CDS             complement(3760..3933)
                     /locus_tag="VFA54_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50341.1"
                     /translation="MKFIKIDKWIVNVSRITYIEKYQSGGVSVRFSGSQDLRLDKPEA
                     DALIATLDASEPR"
     gene            4254..4916
                     /gene="clpP"
                     /locus_tag="VFA54_05745"
     CDS             4254..4916
                     /gene="clpP"
                     /locus_tag="VFA54_05745"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZS50342.1"
                     /translation="MTSQILVPMVIEQTSRGERAYDIYSRLLKENIIFLGTPIDDAIA
                     NMIIAQLLFLEAEDSQKDISIYINSPGGSITAGLAIYDTMQFIKPDVTTICLGQAASM
                     AAVLLAAGTKGKRFALPHSRIMIHQPSVSGLAGQATDIDIYAREILRMRETLNQILAT
                     HTGQPVEKVAHDVDRDYIMEPQDAIKYGMIDSLIEHRDLAASGLTVPSVASRQDGSLL
                     SR"
     gene            5122..7443
                     /locus_tag="VFA54_05750"
     CDS             5122..7443
                     /locus_tag="VFA54_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50343.1"
                     /translation="MRVRIPSRWLSKLRAPARAVLAGALLSTVGGASSSQSRVGSLPA
                     AADNAYVEASLCARCHAQIAESYRQTGMGRSLFRPSAANATEDYAKNHEFYHALSDTH
                     YAMILRNGAYYQRRWQIGFDGKETNIEEMKIDYVIGSGNHARSYLHRTPRGTLIELPL
                     GWYAENGGYWGMSPGFDSRHPQTRRAVSYECMFCHDAYPRIPAGHEAPGSEPVYAGDL
                     PEGIDCQRCHGPGGKHVRMVQAPGAKLAQIRDSILNPARLSPAAQMEVCMQCHLEPTS
                     SRLPSLLRRFDRGPFRFTPGEPLGNFLIYFDHAPGTGHDDKFEIAGAAYRFRKSRCFL
                     ESKGKLTCTTCHDPHRIPRGQEAIRQYSGACRQCHRGRIEALAASGKHPVAGDCISCH
                     MPKRRTEDAVHVVMTDHLIQRGPPAPGLLAELAERHPSEAEEYHGEVVPYYPSPLPPT
                     GENALYRAVAQVQYQSNLQDGLKDLAREIGRQRPREPEFYMQLGAAWRSGGKPEEAVA
                     AFQQAVRLKPDSPRALRCLAEAWRAAGRAQRSAEALDRAIRIAPAEAASWYQYGLLEA
                     GLGRAEQAVEKLRKAISLDPDLPGEYTSLAGILAGAGQIEAAEGALGEALRIDPYDSM
                     AYDLKGRVSAAKGDMQQAVYNFEKATRLRPGYGPHLYDYALALARVNRFDDAQGQVEA
                     ALQADPNLAEAHELAGTLLARKRLLAEAAREYREAVRLRPDFSQAHLDLARVLAAQGD
                     RPGAIEHLREAARGRDAGVARQAAQALQQLGAQ"
     gene            7440..9422
                     /locus_tag="VFA54_05755"
     CDS             7440..9422
                     /locus_tag="VFA54_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZS50344.1"
                     /translation="MKTSPLLTAVSAGVIGLAFCLAPFVFEGKPAAAQQQSGAALHGR
                     VLDAAGRPVAGAMVSLKKKGAGQALLSMTAAGGGYSFPELDSGADYELNADYEILSSG
                     VKVLHVSNSDEKFAIDLTVAAPIQFQEIGARAGLNFVLRNGETGHAYQPEIMLGGVAA
                     LDYNNDGCMDIFFTNGAELPSLRKTGPEFYNRLYRNNCDGTFTDVTEKAGLAGEGYSM
                     GAAAGDYDNDGYVDIFVAGVYRNTLYHNRGDGTFEDVTDKAGLGAPDPEYGKLWSTSA
                     GWFDADNDGYLDLFISNYVSWQPGGDNCTDRGKPYYCHPRVYKPLPNQLFHNNHDGTF
                     TDVSAASGIRRSAGKGMGVVFGDFNGDGLADVFVANDSVPNFLFQNLGGGKFKEIGVE
                     AGVAYAAHGKAVAGMGADFRDFDDDGREDIVLDGIYFDTFPLYRNVGKPGFFVDETAA
                     SGLAAATQDLTGWSLGMYDLDNDGHKDLFFANSHFPGSEPYAHSDAAIPNHVLRNLGN
                     GRFVDVSIGAGRDFQIPALHHGAAFADFDNDGRLDVVVTALNSPVRLFRNISPAAGHW
                     IALRLTGTRSNRDGLGAKVRLTLPSGAAQYNHASTSVGYASSSEPLVRFGLGPYEAAS
                     EIEIRWPSGVVQRLRNISGDRVLVVQEPTAAGRPPS"
     gene            9692..>11011
                     /locus_tag="VFA54_05760"
     CDS             9692..>11011
                     /locus_tag="VFA54_05760"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS50345.1"
                     /translation="MLLALAGHPAIAQVLFGSMVGNVTDPTGAAVPAATVKITEMNTN
                     VSRTVQTNEAGAYTVSTVPAGTYQVEITKEGFRGFVTSNIVVNQNNVVRVDAQLQVGA
                     VAENVEVTAEAAALQTDRADVHAEVSTYALQQLPQPNRTYTGLLTLVPGVTPPGGQLN
                     GGTNNPSKSMQFSANGTGVAGATVRIEGINAQNPWVVQYQSFVPSVEAIENVNVVTNS
                     PDAEQGQAGGASVNVILKSGSNETHGAVYWYHINSATTAVNFFAPPGSKPAHQVDNNA
                     GGSMGGHIIRNKLFYFGSYEGDFLHQANSAIIGVPTPQMLAGDMSGSGTPIYDPNTGN
                     PDGSGKTPYPNNIIPPNQLNPIIGKLTPLIPKPNLPGIVNNYFVNQGTIYNLHKIDTK
                     VDYQANSKLRISGRYGKQPYYNLQQPLFGQILGGNSGGWPSLSFSGAG"
BASE COUNT         2332 a         3366 c         3375 g         1938 t
ORIGIN      
        1 gcaccaacgt cattaaggac ggcgaagagg tgatcggcag ccgcaccaag gtcaagatcg
       61 tcaagaacaa agtcgcggcg ccgttccgct cggcggagtt cgacattctt tatggcgaag
      121 gcatttcgcg ggaaggcgac ctgatcgacc tgggcgcgga gcacaacatc atcgagaaga
      181 gcggcgcatg gtacagctac aagggcgagc gcatcggaca gggccgggag aacgccaagc
      241 aatttctgag agaaaatcag gatatccggg cggatctcga taccgagctg cgccgcattc
      301 ttggcctgct cccggcggtc ccgaacggca gtcccccggc ggaagccact ccggccgcca
      361 aagccggcgc aaatacgaag gcggcagtgc gcggatagcc gggcgctgtt atcggcgggc
      421 agccacgcag cgattggcaa tcagccgtca gcgatcagct agcctgctgg cggcattgct
      481 cgaaaagctc aaagctgaag ctggcggcgc tcctgcaaaa gctgaaggct gagcgctgaa
      541 ggctgactgc tgcatgctgc ccgcggcggc gcccgaagtc aatgatgcac tgcttccttc
      601 ttctcggggt tgagataggc tcgcgaactc gggaaattgg ctgacgaagc caccacgtaa
      661 atcggccttc ctttcatctc ctcgaagatc cgcccgatat attcccccaa gatgccgatg
      721 ctgatgagaa ttgcgccgcc gatgaagcag atcgcgacca tcaatgaggt ccacccgggg
      781 actgtaaaca tgccgagcat gtggcgcacc acagcgttaa tgccctgcgc cattccgatc
      841 acgcccagca ggacgcccag ggcaaaactg agacgcaaag gggttgggga aaacgaaatc
      901 gccgccgtcc aggcgaagcg gagcattttg accagcgggt atttcgtctc acctttcacg
      961 cgcggctgcc gcacgtaggg tacggctgtc tgggcgaaac cgacccaggc caccatgccg
     1021 cgcagaaagc ggtgggtctc gcgcatggcg atcagcgcgt cgagacagcg gcgcgaaatc
     1081 agccggaaat ccccgacatc cacggggaga tcgtggtaaa tcagagcgcg catcagccgg
     1141 taaaaaatcc aggccgtcgc ccgcttgaaa atggtttctc cctgccgcga cgttcggcgg
     1201 ccgtacacca catcgaagcc gcggcagtat tcggccagca tgtcgaggat gacttcgggc
     1261 ggatcttgca ggtctgcgtc catcaacacg acagcgtctc ccgccgcgtg gtccagaccc
     1321 gcggtgctgg cggcctggtg gccgaactgc cgggccagat taacgatctt cacgcgtgaa
     1381 tccgcggccg cccaatccag gagcaaatcg agagtgcgat ccgaactgcc gtcgttgacc
     1441 aggatcagct ccgccgggca cggcagctcg ttcataaagc tcgccagacg catccgcagg
     1501 aagggaatgg cttcctgctc gttccagaca gggatcacga tggaaagcag tcccggatat
     1561 tcccgcggga ttaagtggtg atatgtgtgc gtctgtcgca tgagcgggcg ccttgccact
     1621 tagggagtaa ggcccggttt ctcctctttt ttgacaaaga ggatcgctac cccgtcgtct
     1681 tccacgatgc gccactgcgg gtcggatttc atcagtgtgg cgagcggagt atccggcgaa
     1741 caaagggcta gatcaaaccg gtatttggcg gcaaggtcct tccacgccag atcgccgctc
     1801 cgcagcttca ggtagtccct gacaatcttt tcgccgtagt actgatgacg gccatcgaga
     1861 aaaacacgct ggcggggata gtttctgtaa atcagataat cggcccattg atcgaaggtg
     1921 aacacccggg acgacgccag cgcgtccgcg tggcggctca ccatccggac gggaaaaaac
     1981 tccggggaga agccggcggg ccagtgcatc catccggaca ttccaatcgc cacgagaaag
     2041 ccgagggtca acaggcttgg aaagcgaaag ttaatccgaa gctgcgcggc caaatcgtcg
     2101 aaaatgccgg gaatcgatcg gcgaccccgg gtggaggacc atcggcgcca atagtccgac
     2161 aactggacag caatgaccgg cgtggcgaca atcatgtaaa tcggagcatg ccgcaccgaa
     2221 atcaacgagc agtaagcgag gaacacgatc cacagggcct cgacaatccg ccgcttttcg
     2281 agcaaaggcg ccacgatgat gaggcccaga aacagcagcg ccatgaaatg catcatctgt
     2341 tcggagcgga atgacggcga tttgaactca tccaccagat ccatggtcca cttggagcgg
     2401 atgacgtcaa agaaaatgtg ggcgtgcagc ctgagaccgt aaggattcac cagggaagcg
     2461 gcggcgcagg ccagactcac gccggcgtaa cgcacggcct cggagctgcg gctcaccacc
     2521 ggttcgccgg tccaggcggc ctccgccgcc gagccgatca ccagcagccc gagcagggcc
     2581 agaaacaaca cgaagccgcc gtgcatattg gcccacaagg cggttagcgg agccaacagc
     2641 cagaccgccg ggctcctctc gcggcggtcg gcaaaaatca gccatattcc cgcggtcaga
     2701 aacaggagcg tgaacaggtg cgggcgggcg tggaaatgga tgttgatcgc gttgagcgcc
     2761 atcaagacaa gacccagcgc gatcatgctg tgggcgcccc gccacagcgt atataccagc
     2821 aatatcgtga tgagcgctcc gattacgact cccgaaagca gcgccacgcc tttcagccca
     2881 aagctgcgat gcagcagggc gaagatcagc tccgaaagcc attcgaaagc gatccaatcc
     2941 tgacccggct tggaaaatga aaacaaatcg cggaccggaa cttgatgcgc ggccagtatg
     3001 taatcgccaa tccggatgtg aagcccggtg tctccgtcca taaacagccg ctgccatccg
     3061 ttcgcgcccg cgaggaacga ccacgtcatg acgaagatga aaaacaggtc ggtgtatgac
     3121 ggcgccagca gccgcttcgc caggctaagg agaacccctg gctcgcgatg ggcaacgata
     3181 gagaccgagg ttgacacagt ccagatccga atcggcgagc gctgccggtg cggcggcgca
     3241 tacctttctc gggcggtgct ccgtcaaggg ctcacggcgg ccggcagtgg tccgccgcct
     3301 ggaatgctgg agaaaccggc agcggagacg gccggctccg cggccgaccg gacgcccgca
     3361 gccccggtcc gcccgtaagc cgctgaaaga tcaactcatc ccgagcgaga gtatcacgca
     3421 gagcgaaagg gagtaaataa gagaaggccc taatactaca tcctgaatcc tactgatgcg
     3481 ccttcgaaat aggaccggcg gcgaacacga gctcatcgtc tctcacaaac gccgaatttg
     3541 catccgcccg ccgttgatag ccttcgggaa caggcttact gtctgggaga cgaatcggga
     3601 gcagcgtggc tgccggggcc accaccagat cctggcgcat tcaggcgagt ttctggcggc
     3661 gcgccgcgaa ccattcttcc catgtctcga aatcggaata ccaggacgcc tcgaaaattc
     3721 gggtaagcgc caggccgggg cctcggcccg gccgcgcctt caccggggct ccgatgcgtc
     3781 tagcgttgca atgagcgcat ccgcttccgg cttgtcaagt ctcaagtctt gagagccaga
     3841 gaacctcacg cttacgccgc cgctctgata tttctcaatg taggtaatcc gggacacatt
     3901 cacaatccac ttatcgatct tgatgaactt catagctctc cttcgcactg tcagtcactc
     3961 aaccaatgcc agatacgatt tctgatccct gccgtcatgg cggcatcatc cgcagcgacc
     4021 cgcgcgccct gcaaaaacgg ggatgcacga agtgcagagc caaagccgcc ttcagattct
     4081 cccacttttt cgaggaagca caggtccgcc gggtgagaag aagaccgggg cagcaatggg
     4141 gtatctaaac cggtgattgg agcaatcaga ggttgatttg tctcgacgct aaggcgtcaa
     4201 caccctttct tgcgctttcc aggaacccgc aacgcatgtg agataattaa agtatgacgt
     4261 cgcagatttt ggttccgatg gtcattgagc agacctcgcg aggggagcgc gcttacgaca
     4321 tctactcgcg cctgctgaag gaaaacatca tctttctcgg cacgccgatc gacgacgcga
     4381 tcgcgaacat gatcatcgcc cagcttctgt tccttgaagc ggaggactcg caaaaggata
     4441 tatcgattta catcaattct ccgggcggct cgatcacagc cgggctggcg atttacgata
     4501 cgatgcagtt catcaagccc gatgtcacca ccatctgcct gggacaggcg gcttccatgg
     4561 cggccgtgct tctggcggcc ggcacgaagg gcaagcgctt cgccctgccg cactcgcgca
     4621 tcatgattca ccagccgtca gtgagcggtc tggccgggca ggccaccgat atcgatattt
     4681 acgcccgcga gatcctgcgt atgcgcgaaa cgctcaacca gatccttgcc acgcacactg
     4741 gccagccggt ggagaaggtg gcccacgatg tagaccgtga ctacatcatg gagcctcagg
     4801 acgcgatcaa atacgggatg atcgactctc tcatcgagca ccgggattta gctgcaagcg
     4861 gccttacagt tccctcggtc gcgagccgtc aagacggatc gctgctgtcg cggtagcgag
     4921 gcctccagac caatttcgaa catcgttcgc gagacaacga cgggtttccc gcgcagccag
     4981 cccttatgtt tttcctttca gccggccggc ttcgaggctg tcccagcctc ctgcggtgag
     5041 aaggtggcat ccgtcgcccc atcctgagga ggactgtgta tatcattggt aagccgcttg
     5101 ccctcaaggg gcgcgccgcg catgcgggta aggataccga gccgctggct gtcaaagctt
     5161 cgagcgccgg cacgagccgt gctcgccgga gccttgcttt ctaccgtggg cggggcgtct
     5221 tcctcacagt cccgggttgg ctcgctgccg gcggccgctg acaacgccta cgtcgaggcg
     5281 agcctttgcg cgcgctgcca tgcgcagatc gcggagagct accggcagac cggaatgggc
     5341 cgctcgctgt ttcgcccctc ggcggcaaac gccacggaag actacgcaaa gaaccatgag
     5401 ttctatcacg cgttgtctga cacgcactat gcgatgattt tgcggaacgg ggcctattac
     5461 cagcgccgct ggcaaatcgg tttcgacggc aaagaaacca acatcgagga gatgaagatc
     5521 gattatgtca tcggctcggg caaccacgcg cgatcctatc tgcatcggac gcctcggggg
     5581 acattgatcg aactcccgct cggctggtat gccgaaaacg ggggatattg gggcatgagt
     5641 ccgggctttg actcgcgcca cccacagacg cggcgcgcgg tctcctacga gtgcatgttc
     5701 tgccatgacg cgtatcctcg cattccagcc ggacatgagg cccccggcag cgagccggta
     5761 tatgcgggcg atttgcctga agggatcgac tgccagcgat gccacggccc gggaggcaag
     5821 cacgtccgca tggttcaggc gcccggagcc aagcttgctc agattcgcga cagcattctg
     5881 aaccccgcgc gcctgagccc ggcggcgcag atggaagtct gcatgcagtg tcacctggag
     5941 ccgaccagca gccgtcttcc gtcgctcctg cggcgtttcg atcgcggacc cttccgcttt
     6001 acgcccggcg agccgcttgg aaattttcta atctatttcg atcacgcgcc cggcacgggg
     6061 catgatgaca agtttgagat tgcgggcgcc gcataccggt ttcgaaaatc ccggtgcttc
     6121 ctggagagca aaggcaagct tacctgcacg acgtgtcacg atccgcatcg catcccgcgc
     6181 ggccaggagg cgatccggca gtactcgggc gcttgccggc aatgccaccg cggccggatc
     6241 gaggctctgg cagcttccgg caagcatcca gttgcgggcg attgtattag ctgtcacatg
     6301 cccaagcgca gaaccgaaga cgcggtacat gtggtgatga cggaccattt gatccagcgc
     6361 ggtccgcccg cgcccggcct gctcgccgag ctcgccgaac gccatccgag cgaagcggag
     6421 gagtatcacg gcgaggtagt gccttactac ccgtctcccc tgccccctac gggcgaaaac
     6481 gctctttatc gcgccgtggc ccaagtccag taccagagca acttgcagga cggcctgaag
     6541 gacctggccc gcgaaatcgg caggcagcgg ccgcgcgagc ccgagttcta catgcagctt
     6601 ggagccgcct ggcggagcgg cggaaaacca gaggaagccg ttgcggcctt ccagcaagcg
     6661 gtgcggctca agccggattc cccccgggcg ctgcggtgtc tggccgaggc atggagagcc
     6721 gccggcaggg cccagcgcag cgcggaggcg ctggatcgcg cgatccgaat cgccccggcg
     6781 gaagccgcct cctggtatca atacgggctg ctcgaggccg gtttgggccg ggcggagcaa
     6841 gccgtggaaa agcttcggaa agcgatctcg ctcgatccgg atttgcccgg cgagtacacc
     6901 agcctggcgg gaattctggc cggggcggga caaatcgagg cggcggaagg ggcgctcggt
     6961 gaagcgctgc gcattgatcc ctacgactcg atggcctacg atctcaaagg cagagtctcg
     7021 gccgccaagg gcgacatgca gcaggcggtc tacaatttcg agaaagcgac gcgcctccgc
     7081 ccgggctatg gcccgcatct ttatgattac gccctagcgc tggctcgggt aaatcgattc
     7141 gacgacgcgc aggggcaagt cgaagccgcg ttgcaggcgg atcccaacct ggcggaggcg
     7201 catgagctgg cgggtacact tttggcaaga aagcggctgc tggcggaggc cgcccgcgag
     7261 taccgggaag ccgtgcggct gcggcccgat ttcagccagg cgcatttgga tctggcgcga
     7321 gtgctggcgg cgcaggggga tcgtcccggg gccatcgagc acctgcggga agcggcgaga
     7381 ggacgcgatg ccggcgtggc ccggcaggcg gcgcaggcgt tgcagcaact cggtgcgcaa
     7441 tgaaaacgag ccctctgctg accgccgtaa gcgccggagt cattggactg gcgttctgcc
     7501 tggccccttt cgtcttcgag ggaaagcctg cggcagcgca gcagcagtct ggcgcggctc
     7561 tgcacggccg ggttttggac gccgcaggcc ggcccgtagc gggagcgatg gtgtcgctga
     7621 agaagaaggg cgcaggccag gcattgctgt cgatgaccgc cgccggcggc ggctactcct
     7681 tccccgagct cgacagcggc gccgactatg agttgaacgc ggactatgaa atcctctcca
     7741 gcggagtaaa ggttcttcac gtatcgaatt cggacgagaa attcgccatt gacctcaccg
     7801 tggccgctcc gattcagttt caagagattg gggcgcgagc gggactgaat ttcgtgctgc
     7861 gcaacggaga gactggtcat gcctaccagc cggaaatcat gctcggcggc gtggcggccc
     7921 tggactacaa caacgacggc tgcatggata ttttcttcac caacggcgcc gagctgccct
     7981 ctctgcgcaa gaccgggcca gaattttaca accgcctgta tcgcaacaac tgcgacggca
     8041 cattcaccga cgtgaccgaa aaagccggcc tggccggaga aggctattcg atgggggcgg
     8101 cggccggaga ctatgacaac gacggctacg tagacatttt cgtggccggc gtttaccgca
     8161 ataccctgta tcacaaccgg ggcgatggga ccttcgaaga cgtcaccgac aaggccggct
     8221 tgggcgcgcc ggatcccgaa tacggcaaac tgtggtctac gtcggcggga tggtttgacg
     8281 ccgacaatga cggctacctc gacctcttca tcagcaacta tgtttcctgg cagcccggcg
     8341 gggacaactg caccgaccgt ggcaagccgt actactgcca tccacgggtg tacaagcctt
     8401 taccgaacca gcttttccac aacaatcacg acggaacctt taccgacgtt tccgcagctt
     8461 ccggcattcg ccgcagcgct gggaaaggca tgggagtggt ttttggcgat ttcaacggcg
     8521 acgggctggc cgatgtcttt gtggcgaacg attccgtacc aaatttcctt ttccagaacc
     8581 tcggcggcgg caagttcaaa gaaatcggcg tggaggccgg cgtggcctat gctgcgcacg
     8641 gcaaggccgt cgcggggatg ggcgctgact ttcgggattt tgacgatgac ggccgcgagg
     8701 atattgtgct cgacggcatt tatttcgaca ccttcccact ctaccggaac gtggggaagc
     8761 caggattctt tgtcgacgag accgcagcct cgggacttgc cgccgcgacg caggacctta
     8821 cgggctggag cctcggcatg tacgacttgg ataatgacgg gcacaaggat ctgtttttcg
     8881 ccaactccca ctttcccggc tcggagccct atgctcactc cgacgcggcg attccgaatc
     8941 atgtgctgcg caacttgggg aacggccggt tcgtggatgt ttccatcggc gcggggcggg
     9001 attttcaaat tcctgcgctc catcacggcg cggctttcgc cgacttcgac aacgacggcc
     9061 gtttggacgt ggtcgtcacc gctctgaact ctccagtccg gctatttcgc aatatcagcc
     9121 cggcggccgg ccactggatc gcccttcggc tgacaggaac gaggagcaat cgggacggcc
     9181 tcggcgcgaa ggtccgcctc actctgccca gcggcgccgc ccaatacaac catgcgagca
     9241 ccagcgtggg ctatgcatcc tccagcgagc cgctggttcg cttcgggctc gggccctatg
     9301 aggcggccag cgaaatcgaa atccggtggc cgagcggtgt ggtacaaagg ctcaggaata
     9361 taagcggcga tcgagtacta gtagtacaag agccgacggc ggccgggcgg ccgccatcct
     9421 gagccggcaa cctcactcca gaacggccca gtggagttaa cttccttttt cacctgccga
     9481 ttgcgggcct tcaaaattta ccaacaaagt atttgacaac cggggcgaag ggttatattc
     9541 ttttcccgaa ttggggtcgg ctttagttgt gacaagcaag aagcggtgag ggcaaaaggg
     9601 gaggactatg cagaaaagtc ttggttccag cctaagggta aacctatctg ggtatcaggc
     9661 gtggctttgc ggctcagccg cggcggtact cttgctgttg gcgctggccg ggcatcctgc
     9721 catcgcccag gttctatttg gctccatggt cggcaacgtc accgacccaa ccggggcggc
     9781 agttcccgca gccaccgtga agatcaccga gatgaacacc aacgtctcgc gcacggtgca
     9841 gaccaacgaa gcgggagcgt atacggtatc caccgtgccg gccgggacgt atcaggtcga
     9901 gattactaag gaagggttcc gcggattcgt tacttccaac atcgttgtga accagaacaa
     9961 tgtggtccgc gtggacgcgc agctccaggt gggagcggtc gcggaaaacg tggaggttac
    10021 cgccgaggcg gcggctcttc aaaccgaccg cgccgacgtg cacgccgaag tctcgactta
    10081 cgctttacaa caactgcccc agccgaaccg gacatatacc ggcctgctga ccctggttcc
    10141 gggcgtgacg ccgccgggcg gacagcttaa cggaggaaca aacaatcctt ccaaatcgat
    10201 gcagttttcg gcgaacggca cgggcgtggc cggggcgacg gtccgcatcg agggcatcaa
    10261 cgcccagaat ccctgggttg tgcagtatca gtcgttcgtg ccgtccgtgg aggcgattga
    10321 gaacgtcaat gtggtgacca acagccccga cgcggaacag ggccaggcgg gcggcgcgtc
    10381 ggtgaacgtg atcctcaaga gcggatcgaa cgaaacgcac ggggccgtct actggtatca
    10441 catcaacagc gccacgacgg ccgtcaactt tttcgcgccc ccgggcagca agccggccca
    10501 ccaggtggac aacaacgcgg gcggctcgat gggcggccac atcatccgca acaagctgtt
    10561 ttacttcggc agctatgagg gagattttct gcaccaggcc aattcggcga tcattggcgt
    10621 tccgaccccg cagatgttgg ccggagacat gtccggttcc ggcaccccca tttatgatcc
    10681 caatacgggg aaccctgacg ggagcggcaa gaccccttat ccgaacaaca tcattccgcc
    10741 gaaccagctc aatccgatca tcgggaagct gacgccgcta atcccgaagc cgaaccttcc
    10801 gggaatcgtc aacaactact tcgtcaatca gggaacgatt tacaacctgc acaagatcga
    10861 cacaaaggtc gactaccagg ccaactccaa gctgcgcatc tccggccgct acggcaagca
    10921 gccgtattac aacctgcaac agccgctctt cgggcagatc ctgggcggga acagcggagg
    10981 ctggccgtcg ctttcgtttt caggggccgg g
//
LOCUS       DASREK010000118         9171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_733097, whole genome shotgun sequence.
ACCESSION   DASREK010000118 DASREK010000000
VERSION     DASREK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733097"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFA54_05765"
     CDS             complement(<1..610)
                     /locus_tag="VFA54_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HZS50346.1"
                     /translation="MNPFLRFFLLGTLLLAGAACSRQSESSGRQAGSQAQLEGPAPVN
                     GPVSGGETDDRPVIVAFGDSLTAGLGVDERSSYPAVLQRELDARGYRYRVVNAGVSGD
                     TSGGGVARVEDVLAYRPRIVILELGANDGLRGLPVATTRANLEQIIQTLQRGGARVVL
                     AGMTLPPNYGADYVHSFERVFVDLSKEYNLPRIPFLLEGVAAH"
     gene            646..1341
                     /locus_tag="VFA54_05770"
     CDS             646..1341
                     /locus_tag="VFA54_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS50347.1"
                     /translation="MSIAGPIIKVTNLTKTITSGKNKVEILRGIDFEVSAGSYVAIMG
                     PSGSGKSTLLGLLAGLDSPTTGSIVLDGEEICGLGEDALARLRSRKIGFVFQSYHLIP
                     TLTAEENVLLPAELAGSGNGDNPVRRARELLDSVGLRSRLRHYPVQLSGGEQQRVALA
                     RAFMVRPAILLADEPTGNLDSASGRHVLDLLVALNRRENTTLLLVTHDQDLASNAGRR
                     ITLRDGRIVADES"
     gene            1343..3907
                     /locus_tag="VFA54_05775"
     CDS             1343..3907
                     /locus_tag="VFA54_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZS50348.1"
                     /translation="MIRLRSLSLVMAWRDVRASAGKFLFVIASVAVGVGALTGVRGFS
                     RAFHEALLRDARTLIAADLAVRTFAAPSGEQRQEMQRLAQRGAAVTMVTETLSMISAG
                     SGSTPVMVSLKAVDPALYPFYGAVQLDPPMPLSQALKAETIAVSDDLLLRLGLRAGQS
                     VRIGKADFRIVGVVKREPDRMTGTLNVGPRVLLTHEALARTGLMKFGSRASYRVLVRL
                     GNVPVEFARQRLSKVFGFGARVVDYRQAHPAIDRGLERATTFLSLVSLIALIVGGLGV
                     AMALHSHLQQRMDTIGILKCLGARASQVLRIYLVEALGVGLAGSAAGVLIGYVVQWVF
                     PRLIANYFQIPITIRWASPAALQGLAVGMLCTLLATLPPLLLVRRIKPSDIFRRDMPE
                     ARPSLSDRLHMARAALAAGGSILAGLGGITVWLAGSFRLGLIFLAGVLGAVIALSAFA
                     WLILRLVRIGSAALANCRAGRWRLPSTLRHGIANLHRPGTQAATVLVALGLGVSFMLT
                     VHLVQHSLLVQIFASAPPDMPNVFFINITGRERDGLAELIHSQRGVQDASPPVPLISG
                     KLKSVDGTPVDRLALQGWARRFQGSRYVTWSEEPPRHTEVVEGAWWKGRPHPAEMAID
                     EDAAQILGLRPGSQLEWEMGAKTVAARVAAVYRAEVTRFGSGAEFIFSPGALDGLPAI
                     YYASARVRPADVAALQKAAFERYPTVTVINAAEVLQTVQQVIDQIALIVRFISAFAIF
                     AGAIILAASVAGARLRRLREVSILKTLGATRGCVARIFCVEFLILGTLAGSAGCVLAT
                     GLSSLFLRQLMEARLDINWRANLAAVVFTALAVNLTGWIASVRILNQKPLEILRHE"
     gene            complement(3911..4834)
                     /locus_tag="VFA54_05780"
     CDS             complement(3911..4834)
                     /locus_tag="VFA54_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZS50349.1"
                     /translation="MWKRWTKAIRDWAHEGTPRPQTPPRIGLALGGGFSRSLAHVGVL
                     QVFEQHQIPIHAIAGISSGSIIAAAYASGTPLAEIISKGAVTTFSSYARWTLSRLGLA
                     SSERMQLWLRTILRHTNFEEMQTPLAVVATDLITGAPVVFRDRGDIIAPVRASCSYPG
                     LFLPLQLNGRWLVDGAVSTTVPVQAVRDLGATHVVAVYLHSITVDGFRPANIFQVVSQ
                     CFSILQDRIAADERARAELLLEPKVASFAWNDFERADELVEAGRQSALEALPIIEGWL
                     RQPLASAAPDPGRSITAAPAISSAAGPPLQS"
     gene            4849..7431
                     /locus_tag="VFA54_05785"
     CDS             4849..7431
                     /locus_tag="VFA54_05785"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZS50350.1"
                     /translation="MNLDRYMVKKVTHAPRSRLSPNPAAALIAAALAITAGLLALPAE
                     KTPLTGTPELIQTLDRLNVLGSVLMIAAHPDDENTNVIAYFARGRHIRTAYLSATRGE
                     GGQNLLGTEQNEAMGLLRTQELLAARHIDGGEQFFTRAYDFGFSKNPEEALQKWGRDR
                     LLSDFVRIIRRFRPDVIIDRWPPPPGSGGHGQHTATGYIAPEAFAAAADPNQFPEQMA
                     EHLQPWRARRLVWNTFSFNRRQEIAEEQAADRIRVDAGGFDPVLGKSYAEIAGESRSE
                     HWSQGQGTPERRGPSLQFFVQVAGERVSNPPTSDLFEGIDTTWGRVEGAARLGRLFSQ
                     ARRELVPDQPEKILPLLVAAYTEMERLHDPWVDVKRPELLHAIDLAAGLWLDAEADRW
                     NVVPGSSLDLTLTAINRSHFPLTWERTEIKGVAGQTSAAPNRPLEFNNPQQFKATVTV
                     PPNAPFSQPYWMRELRTADYYPVSDPNLIGMPENPPVLEATFYLRTAQGVVLPMRAPV
                     QYRWIERAGGEKVRAVEVVPPVAVSFTSPAMVFPEARPRPVIVRLSSSVKQAKGTVSL
                     AAPVGWKVAPAAAPFEISDPNQQLGVRFEVTPQARAGGGHMTARAQIGGTTVSAGMVS
                     IQYPHIPPQTYFPPARTRVERFDVRLTAKNIGYVMGAGDEVPQALEQLGASVRLLTGE
                     DLAAGDLRRFDAIVTGIRALNVRDDLIAARQRLLDYVAGGGTLVVQYNTSDARSADRA
                     AALAPYPLTPSAQRTTVEEAPVSFPNPDLPVLHRPNEITARDFEGWVQERGLYFMSGW
                     DPHYRPVFESHDPGEKPQLGGTLYARYGQGVYIYTSYAWFRQLPAGVPGAYRVFANLV
                     SGGK"
     gene            7911..8285
                     /gene="rpsL"
                     /locus_tag="VFA54_05790"
     CDS             7911..8285
                     /gene="rpsL"
                     /locus_tag="VFA54_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZS50351.1"
                     /translation="MPTFNQLVRRGRRPPHWKTASPALESCPQKRGVCTRVYTSTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGIGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGTL
                     DAAGVSDRKQGRSKYGAKRPKS"
     gene            8350..8820
                     /gene="rpsG"
                     /locus_tag="VFA54_05795"
     CDS             8350..8820
                     /gene="rpsG"
                     /locus_tag="VFA54_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZS50352.1"
                     /translation="MPRKGNIDRREVPADPVYNSTLVEKFICSMMWDGKKSTAEKIFY
                     QSMKQLQERANDDPLKVFKKAVENCKPVLEVKTRRVGGANYQVPVEVPQNRRTSLAIR
                     WLISYARLRPEKGMPEKLTAELLDAANNRGGAIKKKDDTHRMAEANKAFAHYRW"
     gene            8863..>9171
                     /locus_tag="VFA54_05800"
     CDS             8863..>9171
                     /locus_tag="VFA54_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZS50353.1"
                     /translation="MARTVPLAKYRNIGIMAHIDAGKTTTTERILYYTGRTHRLGEVH
                     EGTAIMDYMEQEQERGITITSAATTCEWRDCRINIIDTPGHVDFTAEVERSLRVLDGA
                     V"
BASE COUNT         1666 a         2802 c         2968 g         1735 t
ORIGIN      
        1 gatgtgcggc gacgccctcc agcagaaagg gaatgcgcgg caaattgtat tctttcgaga
       61 gatccacgaa aacgcgctcg aaagaatgca cgtaatcggc gccatagttc ggcggcagag
      121 tcatgcccgc gagaactacc cgagcgcccc cgcgctggag agtctggatg atttgctcga
      181 ggttggcgcg agtggtcgcc accggcagcc cgcgcagtcc atcattggct cctagttcga
      241 ggattacgat tcggggacgg tacgcgagca cgtcctccac gcgggcgacg ccgccgccgc
      301 tggtgtcgcc gctgacgccg gcgttcacaa cgcggtagcg gtagccgcgg gcgtccagtt
      361 ccctttggag cactgccgga tagctggatc gttcgtcgac gcccaacccc gccgtcaagc
      421 tgtcgccgaa agcgacgatc acggggcggt catcggtctc gccgccggag accggcccat
      481 ttaccggcgc cgggccctcc agctgcgcct gcgagccggc ctggcgcccg gaagactcgc
      541 tctggcggct gcacgccgcg cccgccagca gaagcgtccc gagaagaaag aagcgaagga
      601 aggggttcac cgcctgcatg ccataataca gttcaggatg ccgctatgtc gatcgcaggt
      661 cccattatca aggttaccaa ccttaccaag acgattacga gcggaaaaaa caaggtggag
      721 attctgcgcg gcatcgattt tgaggtttcg gccggatcgt atgtagcaat aatgggtcca
      781 tccggctccg gcaagagcac gctgcttggg ttgctggccg gcctcgattc accgaccaca
      841 ggcagcatcg ttcttgacgg cgaagaaatc tgcggcttgg gcgaagatgc tctggcgcgg
      901 ctgcgcagcc gcaagattgg ctttgtattc cagtcctatc atttgattcc caccctcacg
      961 gcggaagaga acgtgcttct tccggcggaa ctggcgggca gcggcaacgg agacaatccc
     1021 gtccggcgag ccagggaatt gctcgattcg gtgggactga ggagccgact ccggcattat
     1081 ccggtgcagc tctccggcgg cgagcagcag cgtgtcgcgc tggctcgcgc cttcatggtg
     1141 cgcccggcta ttctgcttgc ggacgagccg acaggcaatc tggattccgc cagcgggcgt
     1201 cacgttctcg atttgctggt cgcgctgaac cgccgggaga atacgacctt gcttctggta
     1261 acgcacgacc aggatctcgc gtccaacgcc ggccggcgca tcaccctgcg cgacggccgg
     1321 attgtagccg acgagagttg aaatgatccg cctccgctcc ctttcactgg ttatggcctg
     1381 gcgcgacgtc cgcgcctcag cgggaaaatt tctgttcgtc atcgcctcgg ttgcagtcgg
     1441 cgtaggagcg ctcactggag ttcgcggctt cagccgcgcg tttcacgagg cgctgttgcg
     1501 cgacgcccgc accctgatcg ccgccgatct tgcggtccgc accttcgcag cgccctcggg
     1561 cgagcagcgg caggagatgc agcggctggc ccaacgcggc gccgcggtca cgatggtcac
     1621 ggaaactttg tccatgataa gcgccgggag cggctcaacg ccggtcatgg tttctctcaa
     1681 ggccgtggat ccggcgctgt atccttttta cggcgccgtg cagcttgatc cgccaatgcc
     1741 gctttcgcag gcgctgaagg ccgaaaccat cgcggtctcc gacgacctgc tgctccgttt
     1801 ggggctgcgc gccggccagt ccgtgcgcat cggaaaagcc gatttccgaa tcgtcggcgt
     1861 ggtcaagcgg gagccggacc gcatgaccgg caccctcaac gtcggaccgc gcgtgttgct
     1921 cacacacgag gctctggccc gcaccggtct gatgaagttc ggcagccggg cctcctatcg
     1981 ggttctggtg cgattgggga acgtgccggt ggaattcgcc cggcagcggc tctcgaaggt
     2041 attcgggttc ggcgccaggg tcgtggacta tcgccaagcg catccggcca tcgatcgggg
     2101 gctcgaacgg gcgacgacct ttctcagcct ggtgagcctc attgcgctga tcgtgggcgg
     2161 cttgggcgtg gccatggctt tgcactcgca cctccagcag aggatggata cgatcgggat
     2221 actgaaatgc ctgggggcgc gcgcaagcca ggtgctccgg atttatttgg tggaagcgct
     2281 gggagtgggc ctagcgggga gcgcggccgg ggtgttgatc ggctacgtgg tgcagtgggt
     2341 ctttccgcgc ctcatagcaa actattttca gatccccatt acgattcgct gggcgtcccc
     2401 ggcggcctta cagggcctcg ccgtaggaat gctgtgcaca cttctcgcta cgctgccgcc
     2461 cctgctcctg gtgcggcgca tcaagccttc cgacatcttt cgccgcgata tgcccgaggc
     2521 ccgcccgtcc ctatcggacc ggctgcacat ggctcgagcc gcgctagctg ctggagggtc
     2581 cattctcgcc ggcctgggag gaatcaccgt ctggctggcg ggatccttcc gcctggggtt
     2641 gatcttcctt gccggcgtat tgggtgcggt gatcgccttg tcagctttcg cttggctaat
     2701 actgcgcctt gtacggatag gttcagcggc gctggcaaac tgccgtgccg gccgctggcg
     2761 gttgccgtcc acgctgcgcc acggcattgc caacttgcac cggcccggga cccaagcggc
     2821 caccgtgctg gtcgcgctcg gtctgggtgt cagtttcatg ctgaccgtac acctggtgca
     2881 acactctctg ctggtccaga tcttcgccag cgccccgccc gacatgccca acgtattctt
     2941 catcaacatc accggccgcg agcgcgacgg cctagcggaa ctgatccatt cgcaacgggg
     3001 cgtgcaagac gccagccctc cggtgccgct gatttcgggc aagctgaaga gtgtggacgg
     3061 cacgccggtc gatcgacttg ccttgcaggg ctgggcgcgg cgattccagg gctcgcggta
     3121 cgtcacctgg tcggaggagc cgccccggca cacggaggtg gttgagggcg cgtggtggaa
     3181 ggggcggccc catccggccg aaatggcgat tgacgaggat gccgcgcaaa ttttggggct
     3241 tcggccggga tcacaactgg aatgggagat gggcgcgaag actgtagctg cgcgcgtcgc
     3301 ggccgtgtac cgggccgagg tgacccggtt cggctccggc gccgagttca tcttcagtcc
     3361 cggcgctctg gacggcctgc cggcgatcta ttacgcgagc gcgcgggtcc ggccagcgga
     3421 cgtggcggct ttgcagaagg ccgcattcga gcgttatccc actgtgacgg tgatcaacgc
     3481 cgctgaggtg ctgcaaaccg tccagcaggt catcgatcag atcgccctga ttgttcgctt
     3541 catttccgcg tttgccattt ttgccggagc catcattttg gcggccagcg tggccggcgc
     3601 caggctccgg cgtctccggg aagtcagcat tcttaagacc ttgggcgcca ctcgaggctg
     3661 tgtggcgcgg atcttttgcg tcgaatttct gattctggga actctggcgg gctcggccgg
     3721 ctgcgtgctc gcgaccggac tttccagcct gtttttgcgc cagttgatgg aggccagact
     3781 ggatatcaac tggcgggcga acctggcagc tgttgtcttc accgcgctgg cggtgaatct
     3841 gactggctgg atcgcgagtg tccggattct caaccaaaaa cctttagaga tactcaggca
     3901 cgagtagaat tcagctttgc aaaggcggcc cagcggcgct ggagatcgcc ggagcggcgg
     3961 ttatagatct gcccgggtcc ggggcggctg aggcgagggg ctggcgcagc cagccttcga
     4021 tgatcggcaa cgcctcgagg gccgactgcc ggccggcctc gaccagttcg tcggcccgct
     4081 caaagtcgtt ccaggcgaat gaggccacct tcggctccag cagcagctcc gcacgggcgc
     4141 gttcgtcggc tgcgatacgg tcttgcagaa tggaaaagca ctggctgacc acctggaaaa
     4201 tgttcgcggg acggaaaccg tccacggtaa tggaatggag atagacggcc actacatggg
     4261 tggcgcccag gtctctgaca gcctgaacag gcacggtagt cgaaacggct ccatccacca
     4321 gccagcggcc gttaagctgg agcggcagga aaaggcccgg ataggaacaa ctcgcccgca
     4381 ctggtgcgat aatgtcacct cggtccctga agactacggg agcgccggtt atcaaatcgg
     4441 tggcgaccac ggccaagggc gtctgcattt cctcaaagtt ggtatgacgc aggatggttc
     4501 gcagccacaa ctgcatgcgc tcgctgctgg ccaggcccag gcgcgacagc gtccatcggg
     4561 cgtagctgct gaaggtcgtg accgcccctt tggaaatgat ttccgcgagc ggggtgccgc
     4621 tggcataggc cgcggctata atcgatccgg aactgatgcc ggcgatcgca tgaatcggga
     4681 tctgatgctg ctcaaagacc tgcaacaccc ccacgtgagc caacgagcgg ctgaaaccgc
     4741 ccccgagggc caggccgatg cgcggcggcg tctgtggcct gggcgtcccc tcgtgcgccc
     4801 aatctcgaat cgccttcgtc cagcgcttcc acatcgctgc ccacccctat gaatttggac
     4861 cgctatatgg tgaaaaaagt tacccacgca ccccggagcc ggctttcgcc gaaccccgct
     4921 gcagccctca tcgcggccgc tctggctatc acggccggac tgcttgccct gcccgccgaa
     4981 aaaacaccgc ttaccggaac ccccgagttg attcaaaccc tcgatcgcct caacgtcctg
     5041 ggcagcgttc tcatgatcgc cgcccaccct gatgatgaga ataccaacgt catcgcctat
     5101 tttgctcgcg ggcgccacat ccgcaccgcc tatctctccg ccacccgggg cgagggcggc
     5161 cagaacctgc tcggaacgga gcagaacgaa gcgatgggtt tgctccgcac ccaggaactg
     5221 ctggcggcgc gccatatcga cgggggagag caatttttca ccagagctta cgacttcggt
     5281 ttcagtaaga atccggagga agcgctgcaa aaatggggcc gggaccggct attgtcggat
     5341 tttgtccgaa tcatccggcg ttttcgtccg gatgtcatca tagaccgctg gcccccgccg
     5401 cccggctcgg gcgggcacgg acagcacacg gcaaccggct acatagctcc agaagcgttt
     5461 gcggcggcag ccgaccccaa ccagttcccg gagcagatgg ccgaacacct ccagccctgg
     5521 cgagcccggc gcctggtctg gaataccttt tcgttcaacc ggcggcagga aatcgcagag
     5581 gagcaggctg ccgatcgcat ccgggtggac gccggcggct tcgaccccgt tctcgggaaa
     5641 tcttacgcgg agattgcggg agaaagccgc agcgaacact ggagccaggg tcagggcacc
     5701 ccggagcgca ggggcccgtc gctccagttt ttcgtgcaag tggccggaga gcgggtaagc
     5761 aatccgccaa catcggatct ttttgaaggt attgatacta cctgggggcg cgtcgaagga
     5821 gccgcccggc tcgggcggct cttcagccag gctcgcaggg agctggtccc tgaccagccg
     5881 gagaagattc tcccgttgct ggtcgccgcc tataccgaga tggagcgcct gcatgacccc
     5941 tgggtggatg tgaagcgtcc ggagttgctc catgccattg acctcgcggc cgggctgtgg
     6001 ctggacgcgg aggcggaccg ctggaatgtc gtacccggct cttctttgga tctcacgctg
     6061 accgcgatca atcggtcgca ttttccgctc acctgggaga gaacggagat aaagggcgtc
     6121 gcgggccaga ccagcgctgc tcccaaccgg ccccttgagt tcaataatcc gcagcagttc
     6181 aaggctacgg taaccgttcc gccaaatgct cccttttcgc aaccctactg gatgcgcgaa
     6241 ttgcgcacgg ccgactatta tccggtttcc gacccaaatc tgatcggcat gccggaaaac
     6301 ccgccggtcc tcgaagcgac gttttacctg cgcacggcgc agggagtcgt gttaccgatg
     6361 cgggcgcccg ttcaataccg ttggatcgaa cgcgcgggcg gcgaaaaagt ccgcgccgtc
     6421 gaggttgtgc cgccggtggc ggtatctttt acttcgccgg ccatggtttt cccggaggcc
     6481 aggccccggc cggtgatcgt gcggctcagc tcgagcgtca agcaagcaaa aggaacggtc
     6541 tcgctcgcgg cgccggtggg ctggaaagtg gcgcctgcgg ccgcgccgtt tgagatttct
     6601 gaccccaatc agcagttggg ggttcgtttc gaggtgacgc cgcaggcgcg ggccggcggc
     6661 gggcatatga cggctcgggc gcaaatcggc gggacgacgg tgagcgccgg catggtttcc
     6721 attcagtacc ctcacattcc gccgcagacg tatttccctc cggcgcggac ccgcgtcgag
     6781 cgcttcgatg tccggttgac ggcaaaaaat atcggctacg tcatgggcgc cggggatgaa
     6841 gtgccgcagg cgctcgagca gctcggcgca tcggttcgcc tgctcacagg cgaagatctt
     6901 gccgcaggcg acttgcggcg cttcgacgct attgtgaccg gaattcgggc tctgaatgtc
     6961 cgcgatgatc tgatcgccgc ccgccagcgc ctgctcgatt atgtggccgg cggcggaact
     7021 ttggtggttc agtacaacac gtccgacgcg cggtccgcag accgggcggc ggcgctggcg
     7081 ccttacccgc tgacaccctc ggctcaacgc acaacggtgg aagaagcgcc cgttagcttc
     7141 cccaatcctg acctgccggt cttgcacaga cccaacgaga tcacggcgcg cgatttcgaa
     7201 ggctgggtgc aggagcgcgg gctatatttc atgagcggat gggatccgca ttaccggccg
     7261 gtgttcgagt cgcacgatcc cggggaaaaa ccgcaacttg gcggcacgtt gtatgcccgc
     7321 tacggccagg gagtatatat ttatacttcc tatgcctggt tccggcagct tccggcggga
     7381 gtgccggggg cctatcgcgt ctttgccaat ctggtaagcg ggggaaaatg atggtgcaga
     7441 ccttgcctcc ggcagcgcaa gggctctatt cccaccgtca ttttcaagcc gtgatccggg
     7501 gaacggagcg caaagcattg gtgtcagctt cggcgggcaa cgggcggtta tctattattt
     7561 ttccgacgcc ttgacataac gaaagttgat tgattaaagt aaaaaagatt agcgagtgag
     7621 gtcttctgct gctgtgctac cgttccgcgg gtgcgggacc cggccggcag ccggccccga
     7681 ggctgcttgc gagacttata gctagcatgg gccgtccgga gttgttttcc tcgcaattga
     7741 aagccggccg agaggggccg ctttcatcgt tcgccgccga gaagggctgc tgccggcaag
     7801 tgcgggtcca tttagggcgg cgtggtttca acctgaactg aactttcgac cgggcttctt
     7861 gcggctcgtg gagtgcgtga ggcgcgggaa ggcggattga ggcttgattc gtgccgacat
     7921 ttaatcaatt ggtgcgccgg gggcgccggc ctccgcattg gaaaacggcc agcccggcct
     7981 tggaaagctg cccccagaag cggggcgttt gcacgcgcgt ttacacgtct acgcctaaga
     8041 agccgaactc ggctctacgc aaggtggcgc gagtgcggtt gactaatgga atcgaggtga
     8101 ccacttacat tccgggcatt gggcacaact tgcaggagca ctcgattgtg ctcattcgcg
     8161 gcggccgcgt gaaggatttg cccggcgtgc gctatcacgt aattcgcgga acgttagatg
     8221 ccgcgggggt ctcggaccgc aagcagggac gcagcaagta tggcgcgaag aggccgaagt
     8281 cgtaacggaa agccgccggc gctcggccgg ctggcctggc tgatagcgga aggctgatag
     8341 ctacttttta tgccacgcaa aggaaacatc gatcgacgcg aagtgccggc ggacccggtc
     8401 tacaactcga ctctggtgga gaagttcatc tgctcgatga tgtgggacgg gaaaaaatcg
     8461 acggccgaaa agatttttta tcaaagcatg aagcagcttc aggagcgggc caacgatgac
     8521 ccgctgaagg tgttcaaaaa ggcggtcgag aactgcaagc cggtgctgga agtgaagacg
     8581 cggcgcgtgg gcggcgcaaa ctaccaggtt cctgtggaag tgccgcagaa ccgccggacg
     8641 agtctggcga ttcgctggct tatctcgtat gcccgtctcc gtcccgagaa gggcatgccg
     8701 gagaagctga ccgctgagct gctcgatgcc gccaacaacc gcggcggtgc gatcaagaag
     8761 aaggatgata cgcatcggat ggcggaggcc aacaaggcgt ttgcccacta caggtggtag
     8821 gaagcggggc ggcgtttctt ccgccgccgc ccgctgagtt ttatggcgcg taccgtacca
     8881 ttagcgaaat accggaatat cggcatcatg gctcacatcg atgccggtaa gaccacgacc
     8941 acggagcgca tcctctatta cacggggcgc acgcaccgtc tgggagaggt ccacgagggt
     9001 acggccatca tggattacat ggagcaggag caggagcgcg ggattaccat tacctccgcg
     9061 gctacgacct gcgaatggag agattgccgc atcaacatca tcgacacgcc gggtcatgtg
     9121 gactttaccg ccgaagtcga gcgcagcctg cgcgtgctcg atggcgcggt g
//
LOCUS       DASREK010000119        15901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_228916, whole genome shotgun sequence.
ACCESSION   DASREK010000119 DASREK010000000
VERSION     DASREK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228916"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /gene="rplV"
                     /locus_tag="VFA54_05805"
     CDS             <1..310
                     /gene="rplV"
                     /locus_tag="VFA54_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZS50354.1"
                     /translation="RVSPQKARLVVDLIRGKHCGDAIHILQATNKRIAPQVEKVLRSA
                     ITNAESASSDVDVDKLYIKRAYVNEGPRMKRLRPAPMGRAYRYQRRLSHIVIEVSDEE
                     "
     gene            323..964
                     /gene="rpsC"
                     /locus_tag="VFA54_05810"
                     /pseudo
     CDS             323..964
                     /gene="rpsC"
                     /locus_tag="VFA54_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            1257..1685
                     /gene="rplP"
                     /locus_tag="VFA54_05815"
     CDS             1257..1685
                     /gene="rplP"
                     /locus_tag="VFA54_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZS50355.1"
                     /translation="MLMPKKVKYRKQQRGRRCGKAWRGSELSFGDFGLKAMEPAWVTD
                     RQIEAARVAMTRFIKRGGKVWLRLFPDKPITKKPAETRMGKGKGAPEQWVAVVRPGKI
                     LFEMEGVPEATAREAMALAAHKLPIPTKFVARRAEGSNER"
     gene            1675..1869
                     /gene="rpmC"
                     /locus_tag="VFA54_05820"
     CDS             1675..1869
                     /gene="rpmC"
                     /locus_tag="VFA54_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZS50356.1"
                     /translation="MSAEKIRELDDRELAHQLAETQDKLFRLRFQMSMGQTDGLKRYR
                     ELKKERARMLTVQRQRELSK"
     gene            1882..2181
                     /gene="rpsQ"
                     /locus_tag="VFA54_05825"
     CDS             1882..2181
                     /gene="rpsQ"
                     /locus_tag="VFA54_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZS50357.1"
                     /translation="MEDQRNKPKGRRAEKVGQVVSTKMAKTIVVEVSRRVPHPLYRRI
                     ITKRTKFYAHDEKGLAKPGDRVRIVETRPLSKLKRWRLAEVVWRAQQVKVEPSEI"
     gene            2269..2637
                     /gene="rplN"
                     /locus_tag="VFA54_05830"
     CDS             2269..2637
                     /gene="rplN"
                     /locus_tag="VFA54_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZS50358.1"
                     /translation="MVGMRTRLEVADNSGARKLACILPRGGDIGLRAGLGDVVTASVK
                     EAAPDSNIKKGSVVRAVIVRTRKETRRRDGTYIRFDSNAAVLINEAGEPIGTRVFGPV
                     ARELRDKKFMKIVSLAPEVL"
     gene            2646..2963
                     /gene="rplX"
                     /locus_tag="VFA54_05835"
     CDS             2646..2963
                     /gene="rplX"
                     /locus_tag="VFA54_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZS50359.1"
                     /translation="MEIKRNDIVKVIAGRDKGKQGRVLSVDPSKRRLLVERVMMSKKH
                     TRRNPAKQIQGGIAEREAPIAVSNVMIVCPSCGPVRIAHHIGPDGRKTRVCRKCGTTL
                     DRK"
     gene            2965..3507
                     /gene="rplE"
                     /locus_tag="VFA54_05840"
     CDS             2965..3507
                     /gene="rplE"
                     /locus_tag="VFA54_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZS50360.1"
                     /translation="MAARLKEHYKQHVAPALRQQFQYASPMAVPKLAKITINIGLGEA
                     TQNARLMDGAVQELALITGQRPVVNKAKKSISAFKLRQGMPIGCSVTLRGERMYEFFD
                     RLVNVALPRVRDFRGVSTKSFDGRGNYTLGLRDQLIFPEIDYAKVDKIKGMNVTITTT
                     ARTDPEALALLKAMGMPFRS"
     gene            3605..3790
                     /locus_tag="VFA54_05845"
     CDS             3605..3790
                     /locus_tag="VFA54_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZS50361.1"
                     /translation="MATTAKIAKELKKPKFKIRLRNRCRICGRPRGFLRKFRLCRLCF
                     RSLALRGEIPGVVKSSW"
     gene            3897..4295
                     /gene="rpsH"
                     /locus_tag="VFA54_05850"
     CDS             3897..4295
                     /gene="rpsH"
                     /locus_tag="VFA54_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZS50362.1"
                     /translation="MNMTDPIADMLTRVRNALAARHPKVDVPASKLKAEIARILKEEG
                     YIANFKVAEEDGKRTIKIYLKYSADNAPVISRIERVSRPGRRVYVGNKEIPRVLGGMG
                     INILTTPRGVMTGRTAHRERVGGEILCEVW"
     gene            4362..4895
                     /gene="rplF"
                     /locus_tag="VFA54_05855"
     CDS             4362..4895
                     /gene="rplF"
                     /locus_tag="VFA54_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZS50363.1"
                     /translation="MSRVGNKAIVLPKGVKVKIEPGAVEVEGPKGKLRAAVPEVIRFE
                     QKDGTLQARREREEDAALHGLARSLVQNAVTGVTTGFTKELDIVGIGYRASVSGRVAT
                     FVLGYSHPIDVLIPDGIDIKVDKQTHIIVSGADKGLVGQMAANIRALRPPDPYKNKGI
                     RYVGEVLKKKVGKTGAK"
     gene            4897..5259
                     /gene="rplR"
                     /locus_tag="VFA54_05860"
     CDS             4897..5259
                     /gene="rplR"
                     /locus_tag="VFA54_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZS50364.1"
                     /translation="MIHQVSRNDLRKRIHRRIRKRLSGSPRRPRLSVYRSLKHIYAQV
                     IDDTTGRTLVSASSAEKDSPLKSGGNVAAAKEIGKLIAERARQAGITQVVFDRSGYLY
                     HGRVKMLAEAAREGGLEF"
     gene            5348..5860
                     /gene="rpsE"
                     /locus_tag="VFA54_05865"
     CDS             5348..5860
                     /gene="rpsE"
                     /locus_tag="VFA54_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZS50365.1"
                     /translation="MPAEQTKRIDPATLNLKDQVVSINRVTKVVKGGKNLSFSALVVV
                     GDPDQKIVGYGSGKAKEVPSAIRKGIESAKKNLVKIHTLQTTIPHRVLGRFGSGMVLL
                     KPAREGTGIIAGGPVRAVIQAAGIANVLTKSIGTTNPHNVVKATFDALLQLRDKQEMA
                     ELRGIEADKL"
     gene            5866..6048
                     /gene="rpmD"
                     /locus_tag="VFA54_05870"
     CDS             5866..6048
                     /gene="rpmD"
                     /locus_tag="VFA54_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZS50366.1"
                     /translation="MSDGKIKIRLKRSPICCPEKHKRVVRGLGFRKLNQVVERPDSSA
                     IRGMIRKIPHLVEVVQ"
     gene            6045..6488
                     /gene="rplO"
                     /locus_tag="VFA54_05875"
     CDS             6045..6488
                     /gene="rplO"
                     /locus_tag="VFA54_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZS50367.1"
                     /translation="MNLSSLHPPKGQKHAPKRIGRGMGSGRGKTATRGSKGQHAGTGF
                     SQKRGFEGGQMPLHRRLPKRGFTNIFKKQFALVNLRQLDRLEGDSFTPERLLELGVIR
                     RTLDGVKILGTGSLTRPITVSAHRFSKSAAEKIAASGGKFEVLGR"
     gene            6485..7882
                     /gene="secY"
                     /locus_tag="VFA54_05880"
     CDS             6485..7882
                     /gene="secY"
                     /locus_tag="VFA54_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZS50368.1"
                     /translation="MKFVETVANIFRVQDLRNRLLFTLALLAVYRVGGHIPTPGVDPA
                     ALEQFFQRNAGTLFGFMDLFSGGQFRRLTIFALGIMPYITASIILQLLTVVIPTLEKL
                     QKEGELGRRKITQWTRYLTVGLSAVQSFGIALTLMKSPGNFVLNPGPGFIAMTMLTLT
                     TGTAFIMWLGEQITERGIGNGMSLIIFAGIVVNLPNAISNLYVNVAVTKQWSVLTLLF
                     ILAMMVIVVAFVVLVERGERRIPVQYAKRVVGRRMMGGQSTHLPMKVNAGGVIPVIFA
                     SSLLTFPQTFGAVEFVRNNRYLSGMLKAISYDEPLYVLLYVIGIILFAFFYVSIIFNP
                     AEVADNMRKYGGFIPGIRPGRRTAEYINEILTKITLVGGLYLALLSVIPDFMIGGIRL
                     HHLPLVGNFFDAHFPRQILEGLNVNFYFGGTSLLIVVGVAMDTINQVEAQLIMRHYEG
                     FTPRSGRIRGRRSTF"
     gene            7951..8631
                     /locus_tag="VFA54_05885"
     CDS             7951..8631
                     /locus_tag="VFA54_05885"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZS50369.1"
                     /translation="MAVQQSPGKRVGAVVLFGPPGAGKGTQARRIAERLGIPHVSTGD
                     MIRQQVKAQSPGGDAFGGMMKKGELIPDEYVNELVRERVKAPDSQRGFVLDGYPRTVG
                     QAQALQSMLAGTGLPIMAFNINIGYNELIPRITGRRSCPRCGAIYNVYLHPPRHDNVC
                     DRDGTPLETRADDREEVIRERLLAYEKQTRPVIDFFRQSGQEMHELDGTLASDKISDQ
                     LVEILNSA"
     gene            8628..9398
                     /gene="map"
                     /locus_tag="VFA54_05890"
     CDS             8628..9398
                     /gene="map"
                     /locus_tag="VFA54_05890"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZS50370.1"
                     /translation="MIVRKTPAELEKMRRSGLLVWQILEELKKMVEPGVTTYDLERAA
                     EKMMADAGARPAFKGYYVPAAGRQYNFVLCTSVNSEIIHGLPSAKRVLREGDIVSIDT
                     GVCLEGYYGDSASTVPVGRISPEAERLLRVTQEALDLAVDKAREGNRLFDISSAVQNH
                     VESNGYSVVREFVGHGIGTKLHEDPQIPNYVDPRNGNPRLKEGMVLAIEPMVCAGKPD
                     TRVLSDRWTAVTKDGSNAAHFEHCVAVTSNGPWILTKP"
     gene            9414..9668
                     /gene="infA"
                     /locus_tag="VFA54_05895"
     CDS             9414..9668
                     /gene="infA"
                     /locus_tag="VFA54_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZS50371.1"
                     /translation="MQRREGADTIRRKGRVIELLPSAMYRVELEDMTRVLAHVGARRS
                     KDFLRLLPGDLVDVELSPLDPRRGRVIGRRRENNGAEPRN"
     gene            9705..9818
                     /gene="rpmJ"
                     /locus_tag="VFA54_05900"
     CDS             9705..9818
                     /gene="rpmJ"
                     /locus_tag="VFA54_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZS50372.1"
                     /translation="MKVRASVKKICNKCKIIRRRGVVRVICVNTKHKQRQG"
     gene            9834..10220
                     /gene="rpsM"
                     /locus_tag="VFA54_05905"
     CDS             9834..10220
                     /gene="rpsM"
                     /locus_tag="VFA54_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZS50373.1"
                     /translation="MARIAGVDLPPQKRAEIGLTYIYGIGRARSNSLLARAGVSPDKK
                     VRDLTEDEVNRIRHILEEEGAVEGDLRKEISMNIRRLIEIGSYRGLRHRRNLPVRGQR
                     THTNARTRKGPRKGTIANKKKATAKT"
     gene            10297..10710
                     /gene="rpsK"
                     /locus_tag="VFA54_05910"
     CDS             10297..10710
                     /gene="rpsK"
                     /locus_tag="VFA54_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZS50374.1"
                     /translation="MATKAAAGKTTKKKTFKKKEKKIVHSGTVFIQATFNNTLVTVAD
                     TQGNVISWSSAGALGFRGSRKGTPFAAQQAALAAANAAKDHGMRTVEVRVKGPGSGRE
                     SAIRALATAGLEVRAIKDVTPIPHNGCRPPKKRRV"
     gene            10803..11438
                     /gene="rpsD"
                     /locus_tag="VFA54_05915"
     CDS             10803..11438
                     /gene="rpsD"
                     /locus_tag="VFA54_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZS50375.1"
                     /translation="MARYRGPVCRLCRREGMKLFLKGERCYSDKCAIEKRNFAPGQHG
                     KSKRPKKLIGYGLQLREKQKVRRIYGLLERQFRNTFKKAAQMKGVTGDNLMSLLERRL
                     DTVVYRMGLGTSRAQARQLVQHGHIHVNGRKVNIPSYEVKPGDTIEVRESSRKNAALL
                     AAREVTSHVSKPSWIDVDLESLKGTINASPKREEMTQIPINEQLIVELYSK"
     gene            11506..12552
                     /locus_tag="VFA54_05920"
     CDS             11506..12552
                     /locus_tag="VFA54_05920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZS50376.1"
                     /translation="MWKGFQKPKRLAANTDTLTDKYGLFWAQPFERGFGTTIGNALRR
                     VLLSSIEGPAITAVKIEGVLHEFSSIPGVVEDATDIILNLKQIPFKVNGEGNKTVRLF
                     SDQPGVVTSGQIQADADVEILDKNMYVATVSEGGKLDVEMRIKMGRGYVSADKNFDED
                     LALGYIPIDSVHSPVRKVNYTVEAARLGQVTDYDKLTLEVWTNGVVAPQDAIGYAAKL
                     LKDHMAIFINFEEQLETEEAAAESAAERLNENLNRSVEELDLSVRSYNCLKNAQIQTI
                     GDLVTRTEADMLKTKNFGRKSLNEIKEILAQMGLSLGMRIDEQGRLVPPPGAGSSPPP
                     GTESEQQIEQPGVE"
     gene            12574..13032
                     /gene="rplQ"
                     /locus_tag="VFA54_05925"
     CDS             12574..13032
                     /gene="rplQ"
                     /locus_tag="VFA54_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZS50377.1"
                     /translation="MRHKRAGYKLKRDMGARKALLRGLTTSVIENERVITTVPKAKAV
                     RPLVEKMITLAKRDTLHTRRQAAAFLETPKAVKKLFETFGPRFAQRNGGYTRITRLGW
                     RRGDGAEQVIIELVGSELVKRAEERRKRREARLRAAQEGGPAEAPEKGDE"
     gene            13277..15265
                     /gene="asnB"
                     /locus_tag="VFA54_05930"
     CDS             13277..15265
                     /gene="asnB"
                     /locus_tag="VFA54_05930"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZS50378.1"
                     /translation="MCGIAGYTHSARRSDAALIRRVTGALRHRGPDQEGVYVSDCVSL
                     GAVRLKIIDLEDGEQPMADDEHQTVLVYNGEIYNYQEIREELIARGHRFRSCSDTEVV
                     LRSFLEWDTACFKKFKGMFGLALWQERQRRLILARDRMGIKPLYFCARAGQIYFGSEM
                     KAILEHPEISRNLDLAGLDQYLSLNYTPAPRTLVEGIQKLLPGHLLEWQAGVVRTEPY
                     WRLQLAPDGTLSEQDAVCQLDALLRRSVRQHLISDVPLGLLLSGGVDSSTIAHYAASE
                     SRVPLKTFSISFKGRTFDESRYFRAVAGRYSTEHYELDLSRDLPLEEAVRQFSEYADE
                     PCGDSSSLPVWYVSRLCRQHVTVALSGEGADELFGGYLTYQADRVAAWMRLVPASVRR
                     AALRAARRWLPVSEDKISLEYMIKRFLEGTFLPPLAAHCYWNGAFSAEQRRMLCAYNP
                     PFLSRELEISDAVGEAPAAWNVEDLDGGSSASRGLNRFLWFDQRYYLPDDILAKVDRM
                     SMAHALEVRPPFLDHEIVEFAARLPVRLKIRGGRQKYILKLLMRDKLPSEILLRKKVG
                     FDIPAHDWLRRELRPLLLDALNPAALERTGLFRPGAVERIIHDHLERKINAGFQLWSL
                     LILLLWMERWQIRSPLERIKDSTPAAELSPAPVSFATP"
     gene            15175..>15901
                     /locus_tag="VFA54_05935"
     CDS             15175..>15901
                     /locus_tag="VFA54_05935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS50379.1"
                     /translation="MANTVTSGTDQRLNAGGRALSGASQLRYTLILVIAGAAIFLGSI
                     FAPPSLMDDVDAVNAQIARNMLDSGDWVTPRIDGVVYLEKSPLNYWLMAASYFVFGVH
                     DWAARLPAALGAVGLMLVTAALGTWGLGRRGGFYAGLAVGTCVGLYLFTRIVIPDVLL
                     TLAVTCALYCFLRAVEGPPAKEGLRPAYNGRLWAYGFWASIGVGMLLKGLLGALVPAA
                     TGGLYLLFRRRFGDRDTWRRLRPV"
BASE COUNT         3411 a         4372 c         5190 g         2928 t
ORIGIN      
        1 gcgggtatcg ccgcagaagg cgcggctggt tgttgacctg atccgcggga agcattgcgg
       61 cgacgccatt cacattttgc aggcgaccaa caagcggatt gcgccgcagg tggaaaaagt
      121 gctgcgctcg gcgattacaa acgccgaaag cgcttcgagc gatgtggatg tggataagct
      181 ctatatcaaa cgcgcgtacg tcaacgaagg gccgcggatg aagcggctcc ggcccgcccc
      241 gatgggaaga gcttaccgat accagaggcg gttgtcccac atcgtaatcg aagtgagcga
      301 cgaagaatag cagcggagga ttatgggtca gaaagttcac ccttatggat ttcggctcgg
      361 ctacaacaag acgtggcact cgcgctggtt tgccaagcag ggctacgccg acttgctcca
      421 cgaagaccta aagctgaaag aggtgctgcg gcagaggctg aaggcggcgg gcatcagctc
      481 ggtggaagtt gaccggccgg gcaacaagct gcggatcacg atccgcacct cgcgtccggg
      541 catcatcatc ggccgcaaag gcgccgaaat tgaaaagctc aagcaggagc tgcaccagcg
      601 cacaaatcgc gaggtgttca tcgacatcca ggaggtgcat aaacccgagc tggacgccca
      661 actggtaagc gagagcgtgg cgctgcaact ggaaaagcgc gtggcgttcc gtcgcgcgat
      721 gcgcaaggcc gtggattcgg cgctgcgttt cggctgccag ggcatcaaga tccgcgtggc
      781 tggaaggctg aatggcgctg aaatcgcccg cagcgaatgg tatctgcaag ggcggctgcc
      841 gctgcacacc ctgcgggccg atatcgacta cggcgcctcg gaagctcaca cgacttatgg
      901 agtgatcgga gtcaagacct ggatctatcg cggcgaaatc atcgagacca aagggcgcaa
      961 agcggcgcta ggatctctcg agccggagcc gctgccggca aggcgccggg agcctcggcc
     1021 ggatgcagcg cagggccggc ctcaggcgaa agaccgagag cgggcgcggc aagtcggccc
     1081 gccgccaccg cccgttcccg ccgcctccgc cgcagcgccg ccggccgctg agcctgcggc
     1141 cgaagagccg ggcacgcgcg ggcctgggcc ggtgacgccg ccgctggctc ccaagccgga
     1201 gaccgctgcg ccatcgggcg gcgcgggcga agaagcgaac aagaaggaat ccagccatgt
     1261 tgatgcccaa gaaagttaaa tacagaaagc agcagcgggg ccggcgctgt ggcaaagcgt
     1321 ggcgcggctc cgagttgtcc ttcggcgatt ttggcctgaa ggcgatggag ccggcctggg
     1381 tcacggaccg gcagattgag gcggctcgcg tggcgatgac ccgctttatc aagcgcggcg
     1441 ggaaggtctg gctgcggctg tttcccgata agccgatcac caagaagccg gcggaaaccc
     1501 gcatgggcaa aggcaagggc gcccccgaac aatgggtggc ggtggtcagg ccggggaaaa
     1561 ttctgttcga aatggaaggc gtgccggaag ccacagcgcg tgaggcgatg gcgctcgcgg
     1621 cgcataagct tccgatccct actaaattcg tcgcgcggcg cgcggagggg agcaatgagc
     1681 gctgagaaaa tccgcgaact cgatgaccgg gagctggcgc atcaactggc ggagacgcag
     1741 gacaagctgt tccggcttcg tttccagatg tccatgggac agacggacgg gttgaagcgg
     1801 tatcgcgaac tgaagaagga gcgcgcccga atgttgaccg tgcagcgcca gcgggagttg
     1861 tccaagtagc cgggagtgag catggaagat caacggaata agccaaaagg cagacgggcg
     1921 gaaaaggtcg gccaggttgt ttccaccaag atggccaaga cgattgtcgt cgaagtgagc
     1981 cgccgcgtgc cgcacccgct ctatcggcgc attatcacca agcgcactaa gttttacgcc
     2041 cacgatgaaa aagggctagc gaagccggga gaccgggtgc gaatcgtgga aacgcggccg
     2101 ctgagcaagc tgaaacggtg gcgtctggcg gaggtggtgt ggcgggcgca gcaggtgaaa
     2161 gtcgagccga gcgagatttg agaaaggggt cagcagtcag gagcccggag agggaggatg
     2221 tttttcccgg atgcccgttc gggcttttga ttactgggtg aatgagatat ggttggcatg
     2281 cgaacgaggc tggaggtggc cgacaactcc ggcgcgagaa aactggcttg catactgccg
     2341 cgagggggcg atatcgggct gcgggccggc ctgggtgacg tggtgacggc cagcgtgaaa
     2401 gaggctgctc ctgattcgaa tatcaagaag ggcagtgtgg tgcgggcggt gatcgtcaga
     2461 actcgaaaag agacgagacg ccgggacggg acctacatcc gcttcgactc gaacgccgct
     2521 gtgttgatta acgaggcggg cgagccgatc ggcacgcgcg ttttcgggcc tgtggcgcgc
     2581 gagcttcgcg acaagaaatt tatgaaaatc gtgtcgctcg ccccggaggt gctgtgatgg
     2641 gcgccgtgga gatcaagaga aacgacattg taaaggtgat tgcggggcgc gataagggca
     2701 agcaagggcg ggtgctgagc gtcgatccgt cgaagcggcg gctgctggtc gaacgggtga
     2761 tgatgagcaa aaaacatacg cggcgcaatc cggccaagca gattcagggc ggcatcgctg
     2821 agcgcgaggc gccgatagcg gtttcgaacg tcatgatcgt gtgcccgagc tgcgggccgg
     2881 tgcgcatcgc ccatcatatc gggcccgacg gccgcaagac gcgggtttgc cgcaaatgcg
     2941 gaactacgct ggacaggaaa taacatggcc gccagactga aagagcacta taagcagcat
     3001 gtagcgccgg cgctccggca gcagtttcag tatgcgagcc ccatggctgt ccccaagctg
     3061 gcgaagatca cgatcaatat cgggttggga gaagcgacgc aaaacgccag gttgatggat
     3121 ggggctgtgc aggaactggc gctcatcacc gggcagcgcc cggtggtcaa caaggccaag
     3181 aaatcgattt cggcgttcaa gctgcggcag ggcatgccga tcggctgctc ggttacgctg
     3241 cgcggcgagc ggatgtatga gttcttcgac cggctggtga acgtggcgct gccgcgcgtg
     3301 cgcgactttc ggggagtctc gacgaaatcc tttgacgggc gcggcaacta tacgctcggc
     3361 ctgcgcgacc agcttatttt ccccgagatt gattatgcca aggtggacaa aatcaaagga
     3421 atgaatgtga cgatcaccac cacggcgcgg acggacccgg aagcgctggc cctgctgaaa
     3481 gccatgggca tgcccttccg cagttgattg atttggccgc accagacggc gtccggctct
     3541 tccgactttg caagggaggt gcgggccggg cttgtgagtg actgctggtg aaaaaggacg
     3601 aagaatggca accaccgcga aaatcgcaaa ggagctgaaa aagccgaagt tcaagattcg
     3661 actgcgcaac cgttgccgca tctgcgggcg gccgcgaggc tttctccgca aatttcgcct
     3721 ttgccggctg tgtttccgga gcctggcgct gagaggcgaa atcccgggag tggtcaagag
     3781 cagttggtaa agagccggtc tcaactatcg gctttggccg gccaggatac ggatcgctgg
     3841 caggcagagc ggaaagctga tgatcgaggg ttaatcacct atagctgaga gcttctatga
     3901 atatgactga tccgatcgcc gacatgctga cccgggtgcg aaacgccctg gcggcccgtc
     3961 acccgaaggt ggatgtgccg gcgtccaaat tgaaggcgga aatcgcccgg atcctgaaag
     4021 aagaaggcta tatcgccaat ttcaaggtcg ccgaagaaga cgggaagcgg acgatcaaga
     4081 tctatttgaa gtacagcgcc gataatgcgc cggtgatatc gcggatcgag agggtttccc
     4141 ggccggggcg ccgggtctac gtcggcaata aagagattcc tcgcgtgttg ggcggcatgg
     4201 ggatcaacat cctgactacg ccgcggggcg ttatgaccgg ccgcaccgct caccgggagc
     4261 gtgtgggcgg cgagattctc tgcgaggtct ggtaaacaac gaggtccggt aaacaaaagg
     4321 agacgcggcg gctcggagat gcggccgcgc ggtgaggcaa gatgtcgcgt gtaggaaaca
     4381 aggccatcgt gctcccgaag ggagtcaagg taaagatcga gccaggcgca gtggaagtgg
     4441 aagggccgaa gggcaagctg cgagccgcgg tccctgaggt catccgtttc gagcagaagg
     4501 acggaacgtt gcaggctcgc cgcgagaggg aggaagatgc ggccctgcat ggtctggcgc
     4561 gaagcttggt ccagaacgcg gtcaccggag tgaccaccgg atttacgaaa gagctcgata
     4621 ttgtcggaat cggctaccgc gcgagcgtga gcgggcgggt ggccacgttt gttctcggct
     4681 attcccatcc gattgacgtg ctgatacccg acggcatcga catcaaggtg gacaaacaga
     4741 cgcacatcat cgtctccggc gcagacaagg gcctggtagg acagatggcg gcgaatatcc
     4801 gggcgctgcg cccgcccgac ccttacaaga acaagggcat tcgctacgtg ggcgaggtgt
     4861 tgaagaagaa agtcggaaag acgggagcca agtaagatga ttcatcaggt ctccagaaac
     4921 gatctgcgaa aacgcatcca tcggcgtata cgcaaacggc tgagcgggtc gccgaggcgt
     4981 ccgcgcttga gcgtctaccg gtctttgaaa catatttacg cgcaagtgat cgacgatact
     5041 accggccgca cgctggtctc ggccagttcg gcggagaaag actccccgct gaaatcaggc
     5101 gggaacgtgg cggcggcgaa ggaaatcggc aagctgatcg ccgaacgggc gcgccaggca
     5161 ggcatcaccc aggtggtgtt cgaccgcagc ggctatctgt atcacgggcg cgtgaagatg
     5221 ctggctgaag cggcgcgcga gggaggcctg gagttttgat gccgcccggc ggcccccagc
     5281 acggccggac gcgtttcccg caccgcgctg gagcggctca ggatcgtatt tggggaaatg
     5341 gagcaatatg cccgcagagc aaacaaagcg gattgatccg gcgacactga acctgaaaga
     5401 ccaggtcgtg tccatcaacc gggtgaccaa ggtcgttaaa ggcggcaaaa atctgagctt
     5461 ttcggcgctc gtcgtggtcg gcgacccgga ccagaaaatt gtcggttatg gcagcggaaa
     5521 ggcgaaagaa gtgccttcgg cgattcgaaa ggggatcgaa tcggccaaga agaatctggt
     5581 gaagatccac acgctgcaaa ccacaattcc gcaccgggtt ctgggacgat tcggcagcgg
     5641 catggtcttg ctaaagccgg ctcgcgaggg tacgggcatc atcgccgggg ggccggttcg
     5701 ggcggtgatt caggccgcgg gaatcgccaa cgttcttacc aagtcgatcg gaaccaccaa
     5761 tccccataac gtggtcaaag cgaccttcga cgctttgctg caacttcgcg acaagcagga
     5821 gatggcggag cttcggggga tcgaggcgga caaactctag agcttatgtc tgacggcaaa
     5881 atcaaaatcc ggctgaaacg cagtccgatc tgctgccccg agaaacacaa gcgggtcgtg
     5941 cgcggcctcg ggtttcgcaa gctgaatcag gtcgtggaac gtcccgacag ctccgcgatc
     6001 cggggtatga ttcgcaagat tccgcacttg gtcgaggtgg tgcaatgaat ctgagctcgc
     6061 tccatccccc caaagggcaa aaacatgcgc cgaagcggat cggccgcggc atgggctccg
     6121 gccgggggaa aacggctacg cgcgggtcca agggccagca tgcagggacc gggttctccc
     6181 agaagcgggg gttcgagggc ggacagatgc ctttgcaccg ccgcctgccg aaacgcggat
     6241 tcactaatat cttcaagaag cagttcgcct tggtgaacct gcgacaactc gacaggctgg
     6301 aaggcgatag cttcactccg gagaggctgc tcgagctggg ggtgatccgg cgcaccctcg
     6361 atggcgtcaa aattttgggc acgggctcgc tgacgcgccc cattacagtc tccgcgcacc
     6421 gcttctcgaa gagcgctgcc gaaaagatcg ccgcctcagg cggcaagttt gaggttctcg
     6481 gcagatgaag tttgttgaaa ccgtcgccaa tatttttcgc gttcaggatc tgcgcaaccg
     6541 gctgctgttt acgctcgcgc tcctggccgt ttaccgcgtc ggaggtcata tccctacgcc
     6601 gggcgtggat ccggcggcgc tcgagcagtt tttccagcgg aatgccggca cgctgttcgg
     6661 ctttatggat ttgttttcgg gaggccagtt ccgccggctc acgatctttg cgctggggat
     6721 catgccgtac attacggcct cgatcatcct gcaactgctg accgtagtga tcccgacgct
     6781 cgagaagctg caaaaggaag gcgagcttgg ccgccggaag atcacccagt ggacgcggta
     6841 tctcacggta ggcttgagcg ccgtgcagtc gttcggcatc gccctgactc tgatgaaaag
     6901 ccccggtaat tttgtgctca acccagggcc gggcttcatt gccatgacta tgctaacgct
     6961 gaccaccggc acggccttca ttatgtggct gggggagcag atcaccgagc ggggcatcgg
     7021 caacggcatg tcgctgatca ttttcgccgg catcgtggtc aacttgccga acgcgatcag
     7081 caacctctac gtcaacgtgg ccgtgacgaa gcagtggagc gtgctgactc tgcttttcat
     7141 cctggccatg atggtcatcg tggtcgcttt cgtagtgctc gtcgagcggg gtgagcgacg
     7201 gattcctgtg cagtacgcga agcgggtcgt ggggcggcgg atgatgggag gccaatccac
     7261 gcatctgccg atgaaggtca acgcgggcgg cgtcattccc gtaattttcg ccagttcgct
     7321 gctgacattt ccgcagacct tcggcgcagt agagtttgtc aggaacaacc gctatctcag
     7381 cggtatgttg aaagccatca gttatgacga gccgctctat gtgctgcttt atgtgattgg
     7441 catcattctg ttcgcgttct tctatgtctc gattattttc aaccccgctg aggtagccga
     7501 caacatgcgg aagtacggcg gcttcattcc cggcatacgg cccggcaggc gcactgctga
     7561 atatataaac gagatcctga cgaagatcac gctggtcggc gggctttatt tggctttgct
     7621 gtccgtcatc cctgatttca tgatcggagg catccggctg catcatcttc ccctggtggg
     7681 aaactttttt gacgcgcact ttccgcggca gattttggaa ggccttaacg tcaatttcta
     7741 tttcggggga acgtccttgc tcattgtggt aggggtggcc atggatacga tcaatcaggt
     7801 cgaggctcag ttgatcatgc ggcattatga gggtttcacg ccgagatcgg gccgcatccg
     7861 ggggcggagg agcacgtttt aacattgaag tcatcgggca ggcgcgcgct cttgagccgc
     7921 gggattgcac agcagagcga aggacgacga gtggccgttc aacaaagccc gggtaagcgc
     7981 gtgggggccg tcgttctgtt tgggcccccg ggcgccggaa aaggaaccca ggcccgccgg
     8041 attgccgagc gtctggggat tccccatgtt tccaccggcg atatgattcg ccagcaggtg
     8101 aaggcgcaaa gccccggcgg cgacgccttc gggggcatga tgaagaaggg cgagctgatt
     8161 cccgacgagt acgtgaatga acttgtgaga gagcgggtga aagctcctga cagccagcgt
     8221 ggtttcgtac tcgatggcta tccgcggacc gtgggccagg cgcaggcgtt gcagtcgatg
     8281 ctggcaggca ccggcctgcc catcatggcg ttcaacatca acattggata taatgaactt
     8341 atcccgcgga taaccggacg ccggtcttgc ccccggtgcg gcgcaatcta taacgtttac
     8401 ttgcatccgc cccggcatga taacgtatgc gaccgcgacg ggacgccgct cgagacccgc
     8461 gccgatgacc gcgaggaagt gattcgcgaa agacttctgg cctacgaaaa gcaaacccgt
     8521 ccggtaatcg actttttccg gcaatcgggg caggagatgc atgaactgga tgggacgctg
     8581 gcttcggaca agatttcgga ccagttggtt gaaattttga attcggcatg atcgttcgta
     8641 aaacgcctgc cgagctggag aaaatgcggc gcagcggtct gctcgtgtgg cagatcctgg
     8701 aagagctgaa gaagatggtt gaacccggtg taaccactta cgatctggag cgggccgctg
     8761 aaaaaatgat ggccgatgcg ggcgcacgcc cggccttcaa gggctattat gtgccggccg
     8821 ctgggcggca atataatttt gtcttgtgta catcggtaaa cagcgagatc atccacggcc
     8881 ttccctcggc caagcgcgtt ctgcgggagg gcgatatcgt ctcgatcgac actggggttt
     8941 gcctggaagg ctattacggc gattccgcca gcacagtgcc ggtgggccgc atcagcccgg
     9001 aagctgaaag gctgctgcga gtgacccagg aagccctgga tctggctgtc gacaaagcca
     9061 gggagggcaa ccggctcttt gacatttcct cggcggtgca gaatcacgtc gagtcgaacg
     9121 gatattccgt ggtacgcgag tttgtaggcc atggcatcgg caccaagctg cacgaggacc
     9181 cgcaaattcc caactacgtg gatccgcgca acggcaatcc gcggctcaaa gaaggcatgg
     9241 tgctggctat cgagcctatg gtatgcgccg gcaagcccga cacccgggtc ctctcggacc
     9301 gctggacggc ggtcaccaaa gacggaagca atgcggcgca ttttgagcac tgcgtggccg
     9361 taacatccaa cgggccgtgg atcttgacga aaccgtgagg gcgcggccgg cagatgcagc
     9421 ggcgggaagg agcagatacg atccggcgaa agggcagggt catcgaactg ttgcccagcg
     9481 cgatgtaccg agttgagctg gaagatatga cccgcgtttt ggcccatgtt ggggcgcgtc
     9541 ggagcaaaga ttttttgcgg ctcctccccg gtgacctggt ggatgtggag ctttcgccgc
     9601 tcgacccaag gcggggccgc gtgatcggca ggaggcggga aaacaacggt gcggagccga
     9661 ggaactgacg tcccgcggaa tttggcgaga gcgacggaga gggtatgaaa gtacgggctt
     9721 cagtaaaaaa aatatgcaac aaatgcaaga ttatccgccg gcgtggagtg gtccgcgtga
     9781 tttgcgtaaa caccaagcac aagcagcggc aaggatgagg acgagaggtt tttatggcgc
     9841 gcattgcagg agttgacctg cctccgcaga agcgggcgga gatcgggttg acctacatat
     9901 atggcatcgg ccgggcacgt tccaattcgc ttctggcccg ggcgggcgtg agtccggata
     9961 agaaagtacg ggatctgacg gaggatgaag taaaccggat ccggcacatt ttggaggaag
    10021 aaggcgcagt ggaaggcgac ctgcgcaaag aaatctccat gaatatccgg aggctgattg
    10081 aaatcggttc ctaccggggt ctccggcacc ggcgaaacct gccggtgcgg gggcagcgca
    10141 cccataccaa cgcacgcacc cggaaagggc cgcgcaaggg aacgatagcc aacaaaaaga
    10201 aggcgaccgc caagacgtaa ggagctgtcg gccgtccaac caccggactg aatctgcgag
    10261 tgacgctggt ggcttgaatg cgggcggccc aaatctatgg ccaccaaagc agcagcaggc
    10321 aagacgacca aaaagaagac ttttaagaaa aaggaaaaga agattgtcca cagcgggacg
    10381 gtattcattc aggcgacgtt caacaacact ctggtgacgg ttgcggacac gcagggcaac
    10441 gtcatttcct ggtcgagcgc gggcgctctg ggtttccgcg gctcgcgtaa aggaaccccc
    10501 ttcgccgccc aacaggcggc cctggcggcg gctaatgctg ccaaggacca tgggatgcgg
    10561 accgtggagg tccgtgtgaa gggacccgga tcgggccggg aatcagcgat ccgggcgctg
    10621 gcaaccgccg gccttgaggt acgggccatc aaggatgtga caccgattcc ccacaatggg
    10681 tgccggccgc ccaagaagag acgggtctga gcttatgcgc cggaatcgtg gtcaggatac
    10741 tgccaaaact ggatctggca gaacacaaat cgcaagccgg acggcctctg tgaaggagca
    10801 aaatggctag atatcgcggc ccggtatgcc ggctctgccg gcgcgagggc atgaagcttt
    10861 ttttaaaggg cgagcgctgc tatagcgata agtgcgcgat cgagaagcgt aatttcgccc
    10921 ctgggcaaca tggcaagagc aagcggccga aaaagttgat cggttatgga ttgcaactgc
    10981 gcgaaaagca gaaggtccgc cggatctatg ggctgctcga gcggcaattc cggaacacgt
    11041 tcaagaaagc ggcgcagatg aaaggcgtga ctggcgacaa cctgatgagc ctgctcgagc
    11101 gccgtctgga taccgtggtc taccgcatgg ggctgggcac aagccgggcc caagctcgcc
    11161 agctcgtgca gcacgggcac atccacgtaa acggccgcaa ggtcaatatt ccttcgtacg
    11221 aagtgaagcc cggcgacacc attgaggtgc gcgaatcaag ccgcaagaat gctgctctgc
    11281 tggccgcacg cgaagtcact tcgcatgtga gcaagccaag ctggatcgac gtggacctgg
    11341 agagcttgaa gggcacgatc aacgcgtcgc cgaagcgcga ggaaatgacg caaattccca
    11401 ttaacgagca gctcatcgtc gagctgtaca gcaaataaga ttgcagatcg ccggccggct
    11461 gcggcgtcat tcggcgccgg gcctgccgtg aggaggttcc cacttatgtg gaaagggttt
    11521 caaaagccca agcggttggc ggcgaatacc gacacgctga ccgacaaata cggacttttc
    11581 tgggcgcagc cgtttgaacg agggttcggc acgaccatcg gcaacgccct gcgccgcgtg
    11641 ttgttgagct cgatcgaagg cccggccata acggcggtca agatcgaagg cgttttgcat
    11701 gaattcagct cgattcccgg cgtggttgag gacgccacgg atatcatcct caacctcaaa
    11761 cagattccgt tcaaggtgaa tggcgaaggc aacaagacgg tgcgcttgtt ctccgatcaa
    11821 ccgggcgtgg tgaccagcgg gcagattcag gccgatgcgg atgtggagat tctcgacaaa
    11881 aatatgtatg tcgccacggt aagcgagggc ggcaagctgg atgtcgagat gcgcatcaag
    11941 atgggccggg gatatgtgag cgccgacaaa aactttgacg aggatctggc gctcggctat
    12001 attcccatcg attccgtcca ctcgccggtg cgtaaggtta attacaccgt ggaggccgcg
    12061 cggctcggcc aggtcaccga ttacgacaag ttgaccctgg aggtctggac aaatggggta
    12121 gtcgctccgc aggatgccat tggctacgcg gcgaagctgc tcaaggacca catggccatc
    12181 tttatcaact tcgaggagca gctcgaaacc gaggaagctg cggctgagag cgctgctgag
    12241 cggttgaacg aaaacctcaa ccggtcggtc gaagagctcg atctcagcgt ccgatcatac
    12301 aattgcctca agaacgccca aattcagact atcggcgacc tggtgactcg tacggaagcc
    12361 gacatgctga aaaccaaaaa cttcggccgg aagtcgttga atgaaatcaa agagatactg
    12421 gcccaaatgg ggctttccct cggtatgcgg attgacgagc agggccggct ggtacccccg
    12481 ccgggagcgg gatcatctcc gcctcccgga actgagagcg aacagcagat cgagcagccc
    12541 ggcgtggaat agcgtgaaaa acttggactt ccgatgcgac acaaaagagc gggatacaaa
    12601 ctaaagcggg acatgggagc gcgcaaggct ttgctgcgcg gtctgaccac cagcgttatc
    12661 gagaacgagc gcgtcatcac gacagtcccg aaagcgaaag cagtgcggcc gctggtcgaa
    12721 aaaatgatca ctctcgccaa gagggatacg cttcacacgc gccgccaggc ggccgcgttc
    12781 ctggagacgc ccaaggcggt gaagaagctg tttgaaacct ttggtcctcg cttcgcccag
    12841 cgcaacggcg ggtacacgcg cattacgcgg ttgggctggc ggcgggggga tggcgccgag
    12901 caggtgatta tcgaactggt cggttctgag ctggtgaagc gggccgaaga acgccgcaaa
    12961 cgacgggaag cccggctccg ggcggcgcag gaaggcgggc cggcagaagc accagaaaag
    13021 ggcgacgagt agccaacagg cccggctttg ccctccgggc ctgtccagcc tgccatgccg
    13081 gcggcgaact ccgccaattc caagaccttc cccccgacgc tgtcaatccc caaaaatcct
    13141 ggcccggcca gttgacaagt acggaagcgc cgcatatatt tgagacgcga atcaacgcag
    13201 cctctcggtg gacgcgtggg tgacggggat ccattgagac gcggctaaat gttgttgtga
    13261 gcggggacga tcatcagtgt gcgggattgc gggttacacc cattcggccc ggagatctga
    13321 cgcagcctta atacggcggg tgactggcgc cttacgccat cgcggacccg atcaagaagg
    13381 ggtctatgtg tcggattgtg tttccctcgg cgccgttcgc ctgaagatca tcgatcttga
    13441 agacggcgaa cagccaatgg ccgacgacga acaccagacc gttctcgtct acaacggcga
    13501 aatctacaat taccaggaaa ttcgggaaga gctgattgcg cgcggccatc ggttccgttc
    13561 ctgttccgac acggaggtag tgcttcggtc ttttctggag tgggacacgg cctgtttcaa
    13621 aaaattcaaa ggcatgttcg gcttggccct gtggcaagaa cggcaacggc gcttgatcct
    13681 ggcacgcgac cgcatgggca tcaagccgct ctatttttgc gcccgcgccg gccagattta
    13741 cttcggctcc gagatgaagg ctattctgga gcacccggag atttcccgga acctcgatct
    13801 ggccgggctt gatcagtacc tgtctttgaa ttacacgccg gctccccgca ctctggtgga
    13861 gggaatccag aagctgctgc ccgggcatct attggagtgg caggcgggcg tcgtccggac
    13921 agagccttac tggcgactgc agcttgcacc ggacgggaca ctgagcgagc aagacgccgt
    13981 ttgccagttg gatgccttgc tgcgccgctc cgtcaggcag cacctgattt ccgatgtccc
    14041 tctgggactg ctgttgagcg ggggcgtgga ttcgagcaca atcgcgcact acgcggcatc
    14101 agagtcccgc gtcccgctga agaccttctc gatttccttc aagggccgca cattcgatga
    14161 aagcaggtat tttcgcgcag tagccggccg ctactccacg gaacattacg agctggacct
    14221 atcccgcgat cttcctcttg aggaggccgt acggcagttt tcagagtacg cggacgaacc
    14281 gtgcggcgac tcctccagtt tgcctgtctg gtacgtgtcg cggctttgcc ggcagcacgt
    14341 gacggtcgcg ttatccggag agggggccga cgagctgttc ggcggatatc ttacctacca
    14401 ggcggaccgg gtggcggcct ggatgcgttt ggtcccggcc agtgtgcgcc gcgccgcgtt
    14461 gcgcgccgct cggcgctggt tgcctgtgtc ggaagacaaa atcagcctcg aatacatgat
    14521 caagcgcttt ctcgagggga catttttacc tcctctagcc gctcactgct actggaacgg
    14581 ggcgttttcc gccgagcaga gaaggatgct ttgcgcttac aacccgccgt ttctcagcag
    14641 ggaattggaa atttcagatg ccgttgggga agcaccggcc gcctggaacg tcgaagacct
    14701 cgatggcggc tcctcggcgt cccgaggcct caatcgcttc ctctggttcg atcagcgtta
    14761 ttatttgccg gacgatattc tggccaaggt tgaccgtatg agcatggcgc acgcgctcga
    14821 ggtccggccg ccgtttctcg atcacgaaat cgtggaattt gcggcgcggc tgcctgtgcg
    14881 cctgaagatt cgcgggggcc ggcagaaata catcctgaaa cttctgatgc gcgacaagct
    14941 gccgtccgag atcttgctgc ggaaaaaggt cggcttcgac atcccggcgc acgactggct
    15001 gcggcgggaa ttaaggcctt tgctgctcga tgctttgaac cccgctgctc tggagcgcac
    15061 cggccttttc cggcccggcg cggtcgagcg gatcatccac gaccatttgg aaaggaagat
    15121 caacgccggt tttcagttgt ggagcctttt gatcctgtta ttgtggatgg agagatggca
    15181 aatacggtca cctctggaac ggatcaaaga ctcaacgccg gcggcagagc tctctccggc
    15241 gccagtcagc ttcgctacac cctgattctc gtcattgccg gcgcggctat ttttctcggc
    15301 agcatcttcg cgcctccatc cctgatggac gacgtggacg cggtgaacgc ccagatcgcc
    15361 cgcaacatgc tggattccgg cgattgggtc actccccgca tcgatggcgt ggtctacctc
    15421 gaaaagtcgc cgttgaacta ctggctgatg gctgccagct atttcgtttt cggcgtgcat
    15481 gactgggcag cgcgcctgcc cgccgcgctg ggagcggtgg gtttgatgct ggttaccgcc
    15541 gcgctcggaa cttgggggct gggacgccgc ggcggttttt atgccggcct tgcggtggga
    15601 acctgcgtgg gattgtacct gtttacacgg atcgtaatcc ccgatgtcct gcttacactg
    15661 gccgtcacgt gtgctcttta ctgttttctg cgtgcggtcg aaggaccgcc cgccaaggaa
    15721 ggccttcgtc ccgcttacaa tggccgtctc tgggcttatg gtttttgggc gtcgatcgga
    15781 gtaggaatgc tgctcaaggg gctgctcggt gcgctcgtgc cggctgccac gggcggcctt
    15841 tacttgctct tccggcggcg ctttggggat cgagatacgt ggcggagatt gaggcccgtc
    15901 t
//
LOCUS       DASREK010000120        20440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1051598, whole genome shotgun sequence.
ACCESSION   DASREK010000120 DASREK010000000
VERSION     DASREK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 20440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20440
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051598"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFA54_05940"
     CDS             complement(<1..596)
                     /locus_tag="VFA54_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS50380.1"
                     /translation="MPGADSMLSSPPQDAAAERERLILEHLPQVKLIASRIHDKLPQS
                     VSLEDLVSSGIVGLITAIDNFNPKLNVKLKTYAEYKIRGAILDSLRDLDWAPRNKRRK
                     AKLIEAAILMAEKKHGRSPSEEEIAAELGISLSEYHEWLVEVRAVNLARLEQSGSNDG
                     ETADALRFISDDEDQWPSNLFERSELERLITAALDNMPR"
     gene            complement(608..1723)
                     /locus_tag="VFA54_05945"
     CDS             complement(608..1723)
                     /locus_tag="VFA54_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50381.1"
                     /translation="MNIRAYHSDSVESAIRQAKLELGGDAILLESKETPIEERHRGLY
                     EVRFGFGGTAAPPRPSASRPAGKLFELSQGIEEIRRMLYSYTRTYYLPAPDFFSHPAL
                     ARLYQNLAANEISPELAMRLMASAAAAAKGGASDEEVEQALAEHIGSVVPSTQEIGMP
                     GTRPRVIALVGPAGSGKTTTMAKLAVRFQLERRQNVQLLSADTYRVGAIDQLRTYAAI
                     VGAGWTAAEECEQLAAALDRLRGPAADGGEHPGLIVIDTPGFGFSGLEKIADLAAWLR
                     GAPDVDIHLVVSASLKPRDLRRTTEKYRIFGPRKLLFTKLDETLSFGPLLEEAIRTGW
                     ALSFLSAGQGIPEDLTPATPSVLADLALNRRAGLGGC"
     gene            complement(1767..3812)
                     /gene="flhA"
                     /locus_tag="VFA54_05950"
     CDS             complement(1767..3812)
                     /gene="flhA"
                     /locus_tag="VFA54_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HZS50382.1"
                     /translation="MEAVLNQRTTSNLNIAIPLAVVAVVLIMILPVPPAVLDVLISAN
                     ITLSVITLIVTMYITRPVQFSVYPSLLLLLTLFRLALNISSSRLILLNGNSGTAAAGR
                     VIESFGQFVVGGNYVIGVVIFILLVAIQYVVINHGAVRISEVTARFTLDAMPGKQMSI
                     DSDLNAGLIDEAEARARRQEIGREAEFYGAMDGAVRFTQRDAIASIIITAVNIVAGFL
                     IGVLQHGMPLERALQTYTVLTVGDGLVTVVPALLISVSGGLITTRAATDSRLGADFSR
                     QLFFHPEPLLIGSAVLLVLAAAPGLPGLPFLALGAGVGGIAWNLRRKTAAQAVVPVKP
                     QPASERESLEGLLRVEPLAIEVGLGLVKMVDAAQGGTLLKRIAAVRRQLAGELGFIMP
                     PVRVTDNLTLRSREYVVLLKGAEIARYELRAGCELAINPGTVKEKLPGVETREPAFGI
                     PAMWIEASLVERARTLGYTVVDPPNVLATHLTELIRTHAYELFNRQEAKKLLDRVNEE
                     NPKLVEELVPKLATLAVVQRVLQGLLRERVSIRDGASILEAISESAAMTRNPVLLVEY
                     VRQSIARTVVKPYVNDKGELAAFFVDPAIEETVRAGIEHAEASTRLNLAPAAIREIIE
                     KFKKCLGVLQGPGVVITGAEVRFAMRQICESALPQAVFLSHAEIPATVKLVSLGIIQ"
     gene            complement(3904..4959)
                     /locus_tag="VFA54_05955"
     CDS             complement(3904..4959)
                     /locus_tag="VFA54_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HZS50383.1"
                     /translation="MTDQSQKSEQPTPRRLQQARLEGQVAHSRDLTQAAGFALAAAVL
                     AWAGEALFAGLQESMRGLFAEAFRDGLDAGRIGALLAGLLGGPLAAAAYLGAALLATA
                     LIAHFLQTGFAISPKRLQFDFSKLGPAGRIATLPAQNVREACKALLLFPLFAWACWAV
                     LSQNLGAFLDLPNQTPAAAAALVGHSLGSLSGKAAVVLLALGAWDYFRERQKLLRQLR
                     MTKQEVRQEQRDLEGNPLIKARFRRLQREMARRRMMSRVPKSSVVITNPTHYAVALEY
                     RMERMRAPVVTAKGQNYMALRIRRMAEQHGIPLVENPPLAQALYKGCEVGSEIPVALY
                     HAVAEILAYIFRLTRQR"
     gene            complement(5083..5874)
                     /locus_tag="VFA54_05960"
     CDS             complement(5083..5874)
                     /locus_tag="VFA54_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZS50384.1"
                     /translation="MSDGGALIITEQFLIAFLLVFARVAGLFTFLPLPGSSLFPGAVK
                     AALILLVTLVLLPSAAIRASAENGMGTICVWVLRETAFGMAAGVALQFLNEMLGLAAQ
                     ILGFQAGYSYVNTIDPSTQVDAAILNVLLTLLGGLLFFSLDLHLEVVRALAWNLQTVP
                     VGQSVVGGAGAGLALAMVRLGGVVFDVAVRLAFPVVAVLLLLDLALAFLSYVNGRMQL
                     LSLAFPAKMSVAMVSLAALMAAVPRLYEELARQAMAVACGILRRW"
     gene            complement(5994..6263)
                     /locus_tag="VFA54_05965"
     CDS             complement(5994..6263)
                     /locus_tag="VFA54_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HZS50385.1"
                     /translation="MNTDMAVELARQALITSFVVSAPILALGFVAGVVVSIVQILTSM
                     QDPAFNTIPRLAAFLAGALVLLPWMTERLVSYTIALFSNLKGFAR"
     gene            complement(6324..7049)
                     /gene="fliP"
                     /locus_tag="VFA54_05970"
     CDS             complement(6324..7049)
                     /gene="fliP"
                     /locus_tag="VFA54_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721149.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HZS50386.1"
                     /translation="MKRIAAALLLAAGRLAAQQGPWNGLRLGGKPGPGEWSAPLEILL
                     WLTLLVLLPAAIISLTPFLRLLVVLHFLRQALGTQSAPSNQVLAGLSLFLTLLLMQPT
                     GARIYEQAIGPLDRGEIGMQEALARAAVPVKGFLAKYVREKDVALFLEISRTPRPRNL
                     DDLDLAVLMPAYVLSELRTAFQIGAVLFLPFLIIDLLVASITLSLGMLQLPPVMISAP
                     FKILLFVVVDGWNLVVGSLVKSF"
     gene            complement(7057..7374)
                     /locus_tag="VFA54_05975"
     CDS             complement(7057..7374)
                     /locus_tag="VFA54_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HZS50387.1"
                     /translation="MLNSIAAVALVLAMLAGLLWWLRRLAGKGGRLCGSSGRHIRVIE
                     SVNLAPGAYLHLIEVSGRALLVSAASQGCELLYELEGLPDGAPAESPWRRALFSKRRN
                     LQT"
     gene            complement(7409..7663)
                     /locus_tag="VFA54_05980"
     CDS             complement(7409..7663)
                     /locus_tag="VFA54_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HZS50388.1"
                     /translation="MPEPEQKLARFYDVPIELSVELDRRTMKVREILGLRTGSLIKMN
                     RSAGDNMSLLLAGVVVGYGEIVVIEDTIGIRITDFLQELK"
     gene            complement(7705..8982)
                     /locus_tag="VFA54_05985"
     CDS             complement(7705..8982)
                     /locus_tag="VFA54_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HZS50389.1"
                     /translation="MPSSFSTALSGLRGSSDAVNVIANNLANMNTIAYKASVAEFSDL
                     IYQQVGQNNAGVPFQIGLGTAPISVIRQFDQGTIQNTSGLLDAAIQGAGFFVVRRGDA
                     TLYTRAGNFQLNKLGQLVDSSSDAVQGWVQDASGTVNTNLPVGDIVIPVGQTLAPKAT
                     GLVRLGANLSATAATGDTFSAPLQVFDSLGSPHILTFTFTKAASGWNLDVTIPGADVG
                     SANTTESILTNAPVNITFDQNGILVNPGTPPPSPPPYNLTIDSSAFNLADGAAPLNIT
                     WSLLDPQGNPTITQFAQASAVATTSQDGLASAQLTTVSIADGGKVVGTFSDGQTKVLA
                     QIALATFISPLTLAAVGGNNYIVSSASGTPAIGTADTGGRGSVVGASLEFSNVDIARE
                     FTALMTAQRSFQANSRVITTADDLNQEAINLKR"
     gene            complement(8994..9302)
                     /locus_tag="VFA54_05990"
     CDS             complement(8994..9302)
                     /locus_tag="VFA54_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZS50390.1"
                     /translation="MTTTTALPPLGSGAAGNPAAGSISNPKTQAGTDTFLQLLVAELK
                     TQDPLDPEKGTDFVTQLAQFNSLEQLISINGNLQALVSEAGSGGAASQNSPAAGQTNK
                     "
     gene            complement(9359..9997)
                     /locus_tag="VFA54_05995"
     CDS             complement(9359..9997)
                     /locus_tag="VFA54_05995"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZS50391.1"
                     /translation="MPHAPNPAVAEGAVSARETPTPAETGDQRAAQPGTAAGREKILE
                     IHNTAILIPGNDAAESSPVKDAPACGAATGPSVSGESAGVFNRAVEKASVSSTPAGSR
                     IDLLLRPEGLGRVAVRLLERGGGVEVAVRSDSQPAKSLLVDSLPSLVEGLRRHGWDIT
                     RTAAGGAESEQRGWSAGQQNQRGYEEKSRRQQAQGGTREDRDPAVLFSLGLP"
     gene            11299..12036
                     /gene="flgF"
                     /locus_tag="VFA54_06000"
     CDS             11299..12036
                     /gene="flgF"
                     /locus_tag="VFA54_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HZS50392.1"
                     /translation="MNQALLTAAAGLRSRLETLELVGNNIANVDTAGFKAGREFYNLF
                     LGAGAEPGLSGDANWMPVIEGSAIDFRQGALTPTAAALDLALDGPGFFAVEGPQGPLY
                     TRNGNFRRSTSGQLETAEGYAVLGQNGRPVQLPPGNVTISPDGAISAGQQGIGRLQVV
                     DFPNPQSLARAGGNYFRSADSAPPLPALRTRIAQGQLEGANVNPAEAAIRLVEVSRQF
                     ETLSRAVSLIANEMDRQAIEQIPAAGA"
     gene            12083..12874
                     /gene="flgG"
                     /locus_tag="VFA54_06005"
     CDS             12083..12874
                     /gene="flgG"
                     /locus_tag="VFA54_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HZS50393.1"
                     /translation="MIRALYSAASGMSAQQLNIDNIANNLANANTTGFKHRRAQFQDL
                     LYQTTLAPGTAAGQQTVVPSGLQVGLGARPVSNEIIFSQGDFIQTGNPLDLVIEGQGF
                     FQIRLPTGDLAYTRAGAFHLDRDGNIVTADGDPIEPQITLPRDALSVTVAADGTVSFT
                     QPGQVQAQQGGTIQLAMFPNPGGLNSLGRNLFQPTQASGDPIVGTPGGLEGLGQLLQG
                     NLEGSNVSVVEEFINMIISQRAYEASSKAVQAADQMYQQANNITR"
     gene            12887..13924
                     /gene="flgA"
                     /locus_tag="VFA54_06010"
     CDS             12887..13924
                     /gene="flgA"
                     /locus_tag="VFA54_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HZS50394.1"
                     /translation="MERSRWKLLMPVGLSILCAISQVLGAGGVPAIETEHESVTAAEL
                     ARVMPEWRQAPPETKILYAPLPGLSRELLRAEVERLAARHGIDRAAGEWPERILIVRR
                     LRPLSDREVEAAIAASLAERCRITPEQIRVQLVGFHSPQVPAGQLRLRAGKASNTGEV
                     EIVPLTWITEEKRSGTLWIRARVSAVASYVVAAHELEPETLIGENDITVRNSAIQASL
                     EDWVRDRSEVIGKRLARRLGAGEKIPRAWLIRPKVVGRGDVVELRLARGLIQLRVPGE
                     AQQDGAAGERIMFRNLQSGRQVAARVEDSQSAEVIAGFDLTKKFPKPTRAGATETTKG
                     AAIEHTPSEAK"
     gene            13896..14612
                     /locus_tag="VFA54_06015"
     CDS             13896..14612
                     /locus_tag="VFA54_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HZS50395.1"
                     /translation="MNTRQAKQSKSPIGGYRQCTAFLAVWVLFGFPMSASDQMTPLDR
                     YIQEAQREAAHVAPSGGSLYAPHGPLAELVGDARARQVDDMVTIVVSETASASNSGTT
                     SSSRKTSASAGVPAIYGHVSNQLSNLASASSASSLAGTGTTSRQSAISTRLSARVTHV
                     LPNGYLVVEGSKEITVNSDRQLITVRGIVRPIDLARDNSVTSDRVADIQILVDGKGVV
                     NDAIRRPNFLYRLLLGLLPF"
     gene            14627..15748
                     /locus_tag="VFA54_06020"
     CDS             14627..15748
                     /locus_tag="VFA54_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HZS50396.1"
                     /translation="MQTESAGTRRRDPTKKLIAGLLAAISSLPAARLGDLTTVEGVRD
                     NMLIGYGLVAGLKGTGDRQQTIFPIQTLTNMLQRMGVTVNPAQMQVRNIAAVMVTATL
                     PPYAEPGARLDVVVSSIGDAASLQGGTLLLTALKASTGDTYAVAQGPLSIGGFTAGTA
                     QTGVQVNHSTVGRIPGGALVERAAPTEPPGNSGLNLQLRRPDFATAARIAEAVNKKFG
                     AGVARAENAAVVRVAMPEAFRSKPVDFIAALEPLEVAAEHPARVVLNERTGTVVLGKD
                     VRIAPVEVLHGNLSVQIVTTLEVSQPSPLSPGQTAVVPQTGVAVNEEKARRVVLKEGA
                     NVEELVQSLIGIGSTPRDVIAIIQSIAAAGALDAVVEVI"
     gene            15749..16090
                     /locus_tag="VFA54_06025"
     CDS             15749..16090
                     /locus_tag="VFA54_06025"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HZS50397.1"
                     /translation="MAGQIESAVWSRIRMLPTQQQQIAEASRQFEALLIERLLQMMRP
                     ATQEDQQGAGRAPETYMEMAEQAMAQALAERGGLGIARLLQRGLEGPKGTDRKSSADK
                     QNEAKSGRPVR"
     gene            16188..16496
                     /locus_tag="VFA54_06030"
     CDS             16188..16496
                     /locus_tag="VFA54_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZS50398.1"
                     /translation="MRIDDNFSPSSASGSNAVRSPSISQAPEVRSSGRPQSAGLDGAT
                     DRASLSALGVHISRALEQDPPEIVAKIGQLQQAVANGTYSVPSSAVSVKIVASALGSF
                     "
     gene            16534..16926
                     /locus_tag="VFA54_06035"
     CDS             16534..16926
                     /locus_tag="VFA54_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50399.1"
                     /translation="MEVTASEDTAALVEIQSRLEALAAALASGKSDDVEQRAGQLDSA
                     LKPLEKLRETFSGKDKTPGSAAGLGPALHRIRAQCAALREAAARGEAAVCALAGILAL
                     LYNAEAGAQYSARGSPSLPSAPRLRAEA"
     gene            17104..19122
                     /gene="flgK"
                     /locus_tag="VFA54_06040"
     CDS             17104..19122
                     /gene="flgK"
                     /locus_tag="VFA54_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZS50400.1"
                     /translation="MANILASLGAAAGNLGAFGQALAVTQNNVGNATTPGYARQRINF
                     EALPFDLLGGESGGVTVQSVQSIRDQFLDQQVITAIQNQSYFQNLAQALTQIEPSFQL
                     SGNLSIGATLDQFFNSFSALSTSPNDFTLRQQVLQSAQTLATAIHSSYNGLTSERAAL
                     DQQAGVLANQINSLTAEVAHLNAARSTADANEDNSANQTRLSQVLEQLAGLVDFQTVR
                     QNDGSLSLVLRGGAPLVAGTASFNLQAVPTASRLEIRDALGNDVSPSVTGGQLGAVLN
                     LRNTKIPAYLGQLNQLAATLADAVNNQLAQGRDLAGVPGKPIFQYTSLAFTGTGRTPG
                     TAGAATPSPPDSVTVSFSGGLSGSITADLGSFFVAAAPPAGLAAGDTVTVNFTSADGA
                     TRASITTTPLSAGDATATIAARLNDQIALSPALAGKFSFSDAGGRLKLMESDTAGQGF
                     TFTASTNDPGFITGLEAGGTVGGESAQEIAGALNAQVAADPALSAAGVRFTVSNGEVR
                     LDAGVSFTATVTDNSNGTGFASGLAGTFTAGGSPAAATFAATGLGNSEIAASGPNSSG
                     GNENALALAQLGSSPIVAGFTPSQFYGRLVSRVGEDSSEAQTSLETENQLLTQAQNLR
                     DSFSGVSLDEEATQLTQFQKAYEATARVITVLDSLADTVINLIGSPLT"
     gene            19119..>20440
                     /gene="flgL"
                     /locus_tag="VFA54_06045"
     CDS             19119..>20440
                     /gene="flgL"
                     /locus_tag="VFA54_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZS50401.1"
                     /translation="MIFPRVTLTMLSRQSVIDLQTIEARRQHLTAEVSSGLRVRNPSD
                     DPAGAAAIIQTNSDFEANAQLLKNLQGLGDQLQSVDGALNQAITLVQRAQSLASEAAN
                     FNQTAETRSSIATEVAGLIQNLITIANTSFGGKYVFAGGQDGVLPFVADPSSTNGVIY
                     RGGTGQRSIAFPGGAQSPVSADGQSIFLNPSDFTGSGRTAGITGAATPTPPVGIGVSF
                     SNGVTGAITADLPSFFVAAAPPSVPAAGDQITVHFISADGAINSSITATLGGGETAAQ
                     IVSALNAQIAVTPALAGKITFSDQSGNLKIVESDTAGQSFTFTATDTGTVTSGLEGGG
                     VIGGESAQEIAAALNAQVALNPELSAAKINFSAANGEIKVTSAESLTFTAVDFDRGTG
                     FVSGMAGTHSIGGSNSANVFQVLTALETALRANDVSGIQATLDDLQAAV"
BASE COUNT         4018 a         6479 c         6300 g         3643 t
ORIGIN      
        1 cgcggcatgt tgtcgagagc cgccgtgatc agtctttcca gctccgaccg ctcgaacaag
       61 ttcgaaggcc attgatcctc gtcatcggag atgaagcgca gggcatcggc ggtttcgccg
      121 tcgtttgagc cggactgttc caggcgggcc aggtttaccg ccctcacctc caccagccac
      181 tcgtgatatt cgctgaggga aatgcccaac tcggcggcga tttcctcttc gctcggcgag
      241 cgcccgtgct tcttctcggc cattaaaatt gccgcttcga tcagcttcgc tttccggcgc
      301 ttgttgcgcg gcgcccaatc gaggtcccgc aggctgtcga gaatcgcgcc gcgaatcttg
      361 tactcggcat aggtcttgag cttcacgttg agcttggggt tgaagttgtc gatggcggtg
      421 atcagcccca cgatgccgga actgaccaga tcctccagac tcacgctctg cggcagcttg
      481 tcgtgaatgc gcgaggcgat caactttacc tgcggcaggt gctccaggat cagtctttct
      541 cgttcggccg cggcgtcctg cggcggcgaa gaaagcatgg agtcagcgcc cggcatcgga
      601 gcccctctca gcagcctccc agccccgctc gtcggttgag cgccagatca gcgaggacgg
      661 acggggtagc cggcgtcaag tcctcgggta ttccctggcc ggcgctcaaa aatgagagcg
      721 cccagccggt gcggatcgcc tcttccagca gggggccgaa ggagagcgtc tcatcaagtt
      781 tcgtgaacag cagctttctc ggcccgaaga tccgatattt ctccgtagtc cgccgcaagt
      841 ccctcggctt gagcgaggcg ctcacaacca agtggatatc cacatccggc gcgccacgga
      901 gccatgcggc aaggtctgcg attttttcca gcccggaaaa cccgaatccg ggagtgtcga
      961 tcactatcag cccgggatgc tcgcctccgt cggccgccgg cccccgcaaa cggtccagcg
     1021 ccgcggccag ttgttcgcat tcctcagccg ccgtccagcc ggcgcccaca atagcggcgt
     1081 aggttcgcaa ctggtcgatc gctcccaccc gatatgtatc ggcagagagg agttgtacgt
     1141 tctggcggcg ctcaagttga aaacggacgg cgagcttcgc catcgtagtc gtttttccag
     1201 agccggccgg ccccaccagg gcgatcacgc gaggccgcgt tccgggcata ccgatttcct
     1261 gggtggatgg cacgacagat ccgatatgct cggcgagggc ttgttccacc tcctcatcgc
     1321 tcgcgccgcc tttcgccgct gcggccgcgc ttgccatgag ccgcatggcc agttcggggc
     1381 tgatttcgtt cgctgcgagg ttctggtaaa gccgggccaa cgccggatga gaaaaaaagt
     1441 caggcgccgg caggtagtac gtgcgggtgt agctatacag catccgccgg atctcctcga
     1501 tgccctggct gagctcgaag agctttccgg ccggccgcga agctgatggg cgcggcggag
     1561 cggccgttcc gccgaaaccg aaccggactt cgtacaatcc tcggtgccgc tcctcgatcg
     1621 gcgtttcttt cgattccagc agaatcgcgt cgccgcccaa ctcgagtttc gcctggcgga
     1681 tcgcggactc tacgctgtcc gaatgataag cccggatatt catcggctgc ccctcccggc
     1741 tcctgccgct tcactgttcg cggcgctcac tgaataatcc cgagagacac cagcttgacc
     1801 gtagccggaa tctcggcgtg cgaaaggaat accgcctgcg gcaaggcgct ttcacaaatc
     1861 tgccgcatgg cgaaacggac ctcggcgccg gtgatcacca ctcccggacc ttgcagaaca
     1921 cccaaacact ttttgaactt ctcgatgatc tcccgtatcg ccgcgggcgc cagattcagc
     1981 cgcgtcgagg cctcggcgtg ctcgatgccc gcgcgcacgg tttcctcaat tgccgggtcc
     2041 acgaaaaacg cggccagttc tcccttatcg tttacatacg gtttcactac cgtgcgggcg
     2101 atcgactgcc ggacatactc cacgagcaaa acgggattcc tggtcatcgc cgcggattct
     2161 gaaatcgctt ccagaatgct cgcgccgtcc cggatcgaga cgcgctcccg caacaaaccc
     2221 tgcaatacgc gctgcacgac ggccagagtc gccagtttcg gcaccaactc ctcaaccagc
     2281 ttcgggtttt cctcattcac gcggtccaga agcttcttcg cctcctgccg gttgaatagc
     2341 tcataggcat gtgtgcggat caactccgtc aaatgggtgg ccagaacgtt cggcgggtcc
     2401 actaccgtgt agcccagggt acgggcgcgc tccaccagcg aggcctcgat ccacatagcc
     2461 ggtatgccga acgcgggctc ccgggtttcc acgccgggca gcttttcctt cacagtcccc
     2521 ggattgatcg ccagttcgca gcctgcccgc aactcgtagc gggcgatttc cgctcctttc
     2581 agcagcacga catactcgcg cgagcgcaga gtcaggttat cagtgacgcg aaccggcggc
     2641 ataatgaacc cgagctcgcc cgcgagctgc cggcgcacgg cggcgatgcg cttcaggagc
     2701 gttcctccct gagccgcgtc caccatcttc accaggccga gccctacttc gatagccagc
     2761 ggctcgacgc gcagcaggcc ttccagactc tcgcgctcgg aggccggctg cggtttgacc
     2821 ggaacaacgg cctgtgccgc cgttttccgg cgcaggttcc aggcgattcc gccgaccccg
     2881 gctcccagcg ccagaaacgg cagccccggc aagcccggcg ccgccgcgag caccagcagc
     2941 acggcggagc cgatcaacag cggctcgggg tggaagaaga gctgccggct gaagtcggcg
     3001 ccgagacggg agtcggtcgc cgcccgcgtg gtaatcaggc cgccggaaac ggaaatgagc
     3061 agcgccggca ctacggtcac caggccgtcg cccacggtca gaacggtgta ggtttgcagg
     3121 gcgcgctcga gcggcatgcc atgctgcaaa acgccgatca ggaagccggc gacgatgttc
     3181 accgccgtga taatgatgct ggctatggcg tcgcgctgcg taaagcgcac agcgccgtcc
     3241 atggctccat aaaattccgc ttcgcgcccg atctcctgcc gccgggcgcg ggcctcggct
     3301 tcgtcgatca ggccagcgtt caaatccgag tcaatcgaca tctgcttgcc tggcatcgcg
     3361 tccagcgtga accgcgcggt cacctcggaa atgcgcaccg cgccatgatt gatcacgaca
     3421 tactgaatgg cgaccagcag gatgaatatc accactccaa tgacatagtt cccgcccacg
     3481 acgaattgcc cgaaagattc gatgacgcgc ccggcggctg cggtgccgct gttgccgttg
     3541 agcagaatca ggcggctgga ggaaatattc agggccaacc ggaacagcgt cagtagcagc
     3601 agcagcgacg ggtatacgct gaattgcacc gggcgggtaa tatacatggt cacgatcagc
     3661 gtgatcactg agagagtaat attggccgag atcaacacgt ccagcacggc cggcggcacc
     3721 ggcaaaatca tgatcagcac gaccgccacc acagccagcg gtatggcgat gttgagattg
     3781 ctggtcgtcc gctgattcag aacggcttcc aattcgggct cctggggcct tgctactgct
     3841 tatacaatcg cctctctctt cctgggccgc cgcacccggg gttttcggcc gggcggcggc
     3901 cctctacctc tgtctggtta atcggaagat gtaggccaaa atttcagcca ccgcgtgata
     3961 aagcgcaacc ggaatttcac tgcccacctc gcaccctttg tacaaagcct gcgcgagggg
     4021 cggattttcc actagcggga tgccgtgctg ctcagccatc cgccgtatgc gcaaagccat
     4081 atagttctgg cccttggccg taacaaccgg cgcgcgcatc ctctccatgc gatattcgag
     4141 cgctacggcg taatgggtcg ggttggtaat aacgacgctg gatttgggca cccggctcat
     4201 catccggcgg cgggccatct cccgttgcag ccggcggaag cgtgctttta tcaggggatt
     4261 cccctccaga tccctctgct cttgccgcac ctcctgctta gtcatacgca gttgccggag
     4321 cagcttctgt ctttcccgga aatagtccca ggcccccaaa gcgagcaaga ctacggcggc
     4381 ttttccggaa aggctgccca gtgaatggcc caccagcgcg gcggccgccg ctggcgtctg
     4441 gttcggcaga tcgagaaacg cgccgagatt ctggctgagc acggcccagc acgcccaggc
     4501 gaacagcgga aaaagaagca gcgccttgca cgcctcacgg acgttttgcg cagggagcgt
     4561 ggctatcctg cccgccgggc cgagcttcga aaaatcaaac tgaagccgct tggggctaat
     4621 tgcgaacccg gtttgcagga agtgggcgat gagggccgtt gcgagcagcg cggcgccgag
     4681 atacgctgcc gccgcaagcg gcccgcccag cagaccagcc aggagagcac cgatgcggcc
     4741 cgcatccaga ccgtcccgaa aggcctccgc aaacagcccg cgcatgctct cttgcaagcc
     4801 tgcgaagagg gcctcgcccg cccatgccag aacggcggcc gccagcgcga atccggcggc
     4861 ctgcgtgagg tcccggctgt gcgccacctg gccttccagc cgggcctgtt gcaagcggcg
     4921 tggggtaggt tgttcgctct tctgcgattg gtcggtcatc ggggttgtcc gttctagctg
     4981 ccggactcac gcgcgcatgt ttgatcggtg aagcgccggc tcgcccaacg gcgggcggcg
     5041 tcacgttgcc ggagaagccc gcttgggaaa ctctgcggct tgtcaccatc gccgcaaaat
     5101 accgcaagcg acggccatcg cttgccgcgc cagttcctca tagagccgcg ggacggcggc
     5161 catcaacgca gccaggctga ccatggctac gctcatcttt gccggaaagg ccaggctcaa
     5221 aagctgcatc cggccattca catagctcag gaacgccagc gccaggtcga ggagcaacag
     5281 aacggcgaca accgggaagg caaggcgtac tgcgacatca aagacaacgc ctccgagacg
     5341 caccatagcg agcgccagtc ctgctcctgc gccgcctacg acagactgcc caacaggaac
     5401 cgtctgcaaa ttccaggcaa gcgcccgaac cacttccagg tgcagatcga gcgagaagaa
     5461 cagcagccct cccagcaggg tgagcaaaac attcagaatc gccgcatcca cctgcgtcga
     5521 agggtcgatc gtattgacgt acgaatagcc tgcctgaaaa ccgagaattt gggcggccaa
     5581 gcccagcatc tcgttcaaga attgcaaggc gacgccagcc gccataccga acgcggtttc
     5641 gcgcaacacc cacacgcaga tcgtgcccat accgttctcc gcggaagcgc gaatggcagc
     5701 cgagggcagc agcaccaggg tcacgagcag tatgagagct gctttgacgg ctcccgggaa
     5761 tagactgctt ccaggcaagg gcagaaaggt gaacagcccg gctactctcg cgaacacgag
     5821 cagaaaggcg atcaagaatt gctccgtaat gatcaacgct ccgccgtcgc tcattgaaga
     5881 tcgaacccga ttgtttgcgc ccatccttcg ccaggccggg gaagcgcgcg gcgaaagctt
     5941 tcgccgacaa aagatgcgcg gcccatcgct gcaacgtcgc cggttgcgcg ccgctagcgc
     6001 gcaaagcctt tcaagtttga aaacagggcg atcgtgtaag agaccaggcg ctcggtcatc
     6061 catggcagca gaaccagcgc tcccgccagg aaagcggcga gccttggaat cgtgttgaat
     6121 gcaggatcct gcatcgaagt caggatttgc acaatgctga ccactacgcc cgcaacgaat
     6181 cccaaggcta gaatcggagc gctcaccaca aaagaggtta tcagcgcctg ccgcgccagt
     6241 tcgacggcca tgtctgtatt cataagatcc tcccgccggc gccgcgccgc ccgatctacg
     6301 gcgcatggga gccggcgcgg ctttcaaaaa ctctttacca gcgagcccac cacgagattc
     6361 cagccgtcta cgaccacgaa cagcaagatc ttaaagggtg cggagatcat gaccggcggc
     6421 aactgcaaca tgccgagaga gagcgtgatc gaagcgacga gcaggtcgat gatcaaaaac
     6481 ggcaaaaaca ggaccgcccc gatctgaaaa gccgttctca gctcggaaag aacataggcc
     6541 ggcataagca ctgcaagatc gagatcgtcc aggttccgcg gccgcggagt gcgggaaatc
     6601 tcgaggaaca gcgccacatc cttttcacga acgtacttgg ccagaaaacc ctttacggga
     6661 acggcggcgc gcgccagggc ctcctgcatc ccgatctcgc cgcgatccaa aggcccgatc
     6721 gcctgctcgt aaatgcgcgc gccggtgggc tgcatcaata acaaagtcag aaacagcgac
     6781 aaaccggcca agacctgatt gctgggcgct gactgcgtgc ccagcgcctg gcgcaggaag
     6841 tggagcacta ccagcagccg cagaaacggc gtcagagaaa tgatcgccgc cggcagcagg
     6901 acgagcagtg tcagccagag tagaatctcg agcggcgccg accattcgcc gggaccgggc
     6961 ttgcctccga gccgcagccc gttccaaggg ccttgctgcg ccgccagccg gcctgccgct
     7021 agaagaagtg cagcggcgat tcgcttcatg ccatcctcat gtctgaagat tcctcctctt
     7081 cgaaaacaga gctcgtctcc aaggactttc agccggcgcg ccgtccggca agccctcaag
     7141 ctcgtaaagc agttcgcagc cctgcgaggc cgccgagact agcagcgccc ggcccgagac
     7201 ctcgatcaaa tgcagatacg ctccaggggc caggttgacg ctctcgatca cgcgaatgtg
     7261 ccggccggaa gagccgcaca accggccgcc tttgccggcc aaccggcgga gccaccacag
     7321 caggccggcg agcatggcaa gcacgagcgc gaccgctgca atcgaattca gcaaccggaa
     7381 ggcccctccg gcttcggcct ccccgacatc acttcagctc ctgcaaaaaa tccgtaatcc
     7441 ggataccgat cgtgtcttcg atgacgacaa tctcgccgta gcccacgacg accccagcca
     7501 gaagaaggct catgttgtcg ccggcggacc ggttcatctt gatcagagag ccggtccgga
     7561 ggcccaagat ttcgcgtacc ttcatcgtcc tccggtccag ctcaacgctc agttcgatag
     7621 gcacgtcgta aaagcgcgcc agtttctgtt ccggttctgg catagtctca tcggccccga
     7681 tttgcaggcg cggcaaatct ggcgtcagcg cttcagattg atggcctcct gattcagatc
     7741 atcagcggtg gtgatcaccc gcgaattggc ctgaaagctg cgttgagcgg tcatcaacgc
     7801 ggtgaactcc cgggcgatgt cgacgtttga gaactcgagg gacgctccta ctaccgatcc
     7861 gcgtcccccg gtgtccgccg tgccgatagc cggagttccg gaagcggagg aaacgatgta
     7921 attgttcccg cccaccgccg caagagttaa aggactgatg aaggtggcca gggcgatctg
     7981 cgccagcacc ttcgtctggc cgtcgctgaa agtgcccaca acctttccgc cgtcggcgat
     8041 cgacaccgta gtcaactgag cgctggccaa tccgtcctgg ctggtggtgg cgaccgccga
     8101 agcctgggcg aattgggtga tcgtcggatt tccctgcgga tcaagcaagc tccaggtgat
     8161 gttcaacggc gctgcgccat cggcaaggtt gaacgcgctg ctgtcaatcg tcaggttata
     8221 gggaggcggg ctgggcggcg gcgttcccgg attgaccaaa atgccgttct gatcgaatgt
     8281 gatgttcacc ggggcattgg tcaaaatgct ttccgtcgta ttagcggatc ccacatcggc
     8341 cccgggtatc gtgacgtcaa gattccagcc cgacgccgcc ttagtgaaag tgaaggtcag
     8401 tatgtgcgga gagccgagtg aatcaaatac ttgtaagggc gcggaaaaag tatcacccgt
     8461 ggcggctgtg gcgctgagat tcgccccgag ccgcaccagg ccggtcgcct tcggcgccag
     8521 cgtttggccc accggaatca caatgtcccc cacaggcaag ttcgtattga ccgtgccgct
     8581 ggcgtcctgc acccagccct ggacggcatc cgaagagcta tcgacgagtt gccccagctt
     8641 gttgagctgg aagttgcccg cccgggtgta gagcgtcgcg tcgccccgcc ggacgacgaa
     8701 aaagccggcg ccctgaatgg ccgcgtcgag caggccgctg gtgttctgga tcgtgccctg
     8761 gtcaaactgc cggatcacgc tgatcggggc cgtgcctaaa ccgatttgaa aaggcacgcc
     8821 cgcattgttc tgccctacct gctggtagat caaatcgctg aactccgcga cgctcgcctt
     8881 gtaagcgatc gtgttcatgt tggcgaggtt gttggcaatc acgttgaccg cgtcggaact
     8941 gcctcgcaag ccggaaagag cagtggaaaa tgaggaaggc atagtcagtt ctcctatttg
     9001 ttagtctgtc cagccgccgg cgaattctgg gaggcagccc cgccggatcc cgcctcgctg
     9061 accagggctt gcaaattccc attgattgag atcaactgct cgagcgagtt gaattgggct
     9121 agctgggtta cgaaatcggt gcctttctcg gggtccagcg gatcttgcgt cttgagctcc
     9181 gctaccaaaa gctgcaaaaa cgtgtctgtc ccagcctggg ttttcggatt ggagatggag
     9241 ccggccgccg gattgcctgc cgcgccgcta ccgagcggcg gaagtgcggt tgtcgtagtc
     9301 atatcgaatc ctcatttctg gttttctcct tcgtaccgag ccgaacttac attctttctc
     9361 acggcagccc gagagaaaac agcaccgccg gatcgcgatc ttcccgcgtt ccgccctggg
     9421 cttgttgccg gcggctcttc tcttcatagc cgcgctgatt ctgctgcccg gcgctccagc
     9481 ctctctgttc tgattcggcc ccaccggctg cggtgcgcgt aatatcccac ccgtggcggc
     9541 gcaaaccctc gaccagcgag gggagtgagt ccacaagaag gctcttcgca ggctggctgt
     9601 cgctgcgaac cgctacttcg accccgcctc cgcgctcgag cagacgaaca gccacacgcc
     9661 ctagaccctc cggacgaagc aacagatcga tgcgcgagcc agccggagtg gaagaaacgg
     9721 aagccttttc gaccgcgcga ttgaagacgc ccgctgattc ccctgaaacc gagggcccgg
     9781 ttgcggctcc acatgccggg gcgtctttga cgggcgaact ttctgccgcg tcattccctg
     9841 gaatcaggat ggccgtgtta tgtatctcga gtatcttttc ccggccggca gcagtccccg
     9901 gctgcgcggc gcgctgatcg cctgtctccg ccggagttgg cgtttcacgc gccgaaacag
     9961 ccccctccgc tacagccgga ttcggcgcgt gtggcaaagc tggcttctcg agcgccccgg
    10021 gagaccctgc atcgggccgc ttttccggct gtccgtgttt ccctgcaccc gcatccggct
    10081 ggcttcccgg agagagtaca gcatccggcc gccgcgccgg cgaggaatca acgtctgcac
    10141 tcgctgtcga gggcggcttt gccgcttcac ttcggatcac ttgccccgtt gctgcgcctg
    10201 gcgcttcctg ccgttgggcc tgaagagtaa aggccgccag caacgaatca gccgggaagg
    10261 aatcgacaga ctgcgcggac agaatcctcg cggtcggttg attttcagga attgcagcgc
    10321 ccgcctgtgg cgcggccgcg gacggcgccg cgggcgggaa cacttgggcg gaaaatgcaa
    10381 aggattgcgc ctgggccggg agcggcgcgg tctctgtccg cttgccggct gccggcggcg
    10441 cgtctgcttc ggtcccgcca gtctttccgg ttggcgcctc tttttcgggt gacagctttg
    10501 gctcgacacc tgctgccaga aatgaaaagg aggaagccga aacggcgtgg agcgcgcggc
    10561 ccgcagcgcc agcggcttcg cctgccgctt tgccgcccgc caactgccta tttgtaataa
    10621 aggagtcttg ccctgcgaac gcagtttccg gggcaggagc gttgatgagc aacggcgcaa
    10681 aaaaccttgg atgcagaggc tggccggcag cgacttccgg cgcggcttct gtttcggaac
    10741 cggaagcggc tgcgattgga aactgactca agccaacgat actccagccg ggagtatgtg
    10801 cgatctgcgg cgccgcagcc gttgtgctgc cgtccccagg ctttccatcg ggaacggctg
    10861 ccgcattctt ttctccgggc caactgcggc gtaccagatg gcggaacgcc gaagaggcgc
    10921 cggcaggggc ctggcggctt tcctcccaat ctgcggcggc cgtcccgtgt ggatagccaa
    10981 ttgccgattg ctggctgctc ttcgagctgt tgccggtccc tgcccgcgcc agccccggca
    11041 tgctggacgc aattccattt gccgggccgg aaagtaacag cggagcagcg atcgccatgc
    11101 gatcttgcct ttgcacggcg cgggccgcag cgggaagaca atcgattctc aggtttttgg
    11161 cattgcgccg cgcggcaaga aagtgccgcc aggcaaaata ttttcgctgc cggcgtcgcc
    11221 gcgcgtttgc aacgccgctg catttcaatc ggttgaacgg gcaggctgtg gtacggggat
    11281 tgcattgacg cgcttggtat gaatcaagcg cttctcacgg cggccgccgg cctgcgctcg
    11341 cggctggaaa cgctggaact ggtcggcaac aacatcgcga atgtagatac ggcgggcttc
    11401 aaggccggcc gcgaattcta caacttgttt ctcggggccg gagcagagcc gggcttgagc
    11461 ggcgatgcca attggatgcc cgtcatcgag ggctctgcca ttgactttcg ccagggcgcc
    11521 ttgacgccca cagcggcagc gctcgatctg gccctcgatg gtccgggttt cttcgccgtc
    11581 gaggggccgc agggccctct gtacacccgc aatggcaatt tccggcgatc caccagcggc
    11641 cagctcgaaa ccgccgaagg ctatgccgtc ttgggccaga acgggcggcc agtccaactt
    11701 cctccgggca acgtcaccat tagcccggat ggcgccatct ccgccggaca acaaggaatc
    11761 gggcgcctgc aagtggtgga tttccccaat ccgcagagct tggcgcgggc cggcggaaac
    11821 tattttcgtt ctgcggattc cgccccgccg ctgccggctc tgcggaccag aattgcgcag
    11881 ggccagctcg agggggctaa cgtaaatcca gccgaggcgg cgatccggct ggtcgaagtg
    11941 tcgcgccaat tcgagacgct ctcgcgcgcc gtatccttga tcgcgaacga aatggaccgg
    12001 caggccatcg agcagattcc tgcggcgggc gcatgacacg gacgccggca gccggagcgc
    12061 ggcgcggtaa aggagatgca aagtgattcg cgcattatat agcgctgcca gcggcatgtc
    12121 cgcgcagcaa ttgaacattg acaatatcgc gaacaatctg gccaacgcca acaccaccgg
    12181 tttcaagcac cgccgggcgc agttccaaga tctgctctat caaaccacgc tggctcccgg
    12241 caccgcggcc gggcagcaga cagtcgtgcc ttcgggactt caggtaggcc tgggcgcgcg
    12301 tcccgtctcc aacgagatca tcttctcgca gggggatttc attcagaccg gcaatccgct
    12361 tgatctggtg attgagggcc agggattctt tcagatccgg ctgcccactg gcgacctcgc
    12421 gtacacgcgt gcgggggcgt ttcacctcga tcgcgacggc aatatcgtca cagccgacgg
    12481 ggatccgatt gaaccgcaga ttacgctgcc tcgggatgcc ctgagcgtta ccgtcgcagc
    12541 cgatgggaca gtcagcttca cccagcccgg tcaggtgcag gcgcagcagg ggggaacgat
    12601 tcaactggcg atgtttccca atccgggcgg actaaatagt ctcggccgca atctgtttca
    12661 gcctacgcaa gcgtcgggcg acccgattgt cggcacaccc ggaggcctgg aaggcctggg
    12721 ccaactgttg caaggcaacc tggaaggctc gaacgtgagc gtcgtggagg aattcatcaa
    12781 catgatcatc agccagcggg cctacgaagc ttcgagcaaa gcagtgcagg ctgctgatca
    12841 gatgtatcag caggccaaca acatcacgcg ttagagatcg agacctatgg aacggtcgag
    12901 atggaaattg ttgatgccgg tagggctttc cattctatgc gccatttcgc aggttctcgg
    12961 ggctggcggc gttcccgcca tcgaaaccga gcatgagagc gtgacggccg ccgaactggc
    13021 ccgcgtgatg ccggagtgga ggcaggcgcc gccggagacg aaaatcctct acgcgcctct
    13081 gccaggcttg tcgcgagagc tcctacgggc ggaggtggag cggctggcgg cgcgccacgg
    13141 gattgatcgg gccgccggag aatggccgga gcgaatcttg atcgtccggc ggcttcgccc
    13201 gctttcggac cgggaagtgg aagccgccat agcggcctcg ctcgccgaac gatgccgcat
    13261 taccccggag cagatccgag tgcaactggt cgggtttcac tctccgcaag tgcccgcggg
    13321 ccaattgcgg ctccgcgcag gaaaggcgtc taacacggga gaggtcgaaa tagtccccct
    13381 cacctggata acggaagaaa agcgcagcgg aaccttgtgg atccgggccc gcgtgagcgc
    13441 tgtcgcatct tatgtcgtcg ccgcgcacga gctggaacca gaaacgttga tcggagaaaa
    13501 cgatatcacg gttcggaata gcgcgattca agcttccctg gaagactggg tgcgcgaccg
    13561 gtcggaagtg atcggcaagc gcctcgcgcg gcggctggga gccggcgaaa aaattccgag
    13621 ggcctggctg atccgcccga aagtggtggg acgcggcgac gtcgtcgagc tgcggctggc
    13681 ccgcggactc atccagttgc gagtcccggg agaagcacag caggacgggg ccgcgggaga
    13741 acgcataatg tttcgaaatt tgcagagcgg gcggcaagta gcggcgcgcg tggaagactc
    13801 ccaaagcgcg gaagtgatcg ctggttttga cctgacaaaa aaattcccaa agccaacgcg
    13861 cgcgggcgca acggaaacga cgaagggggc tgccattgaa cacacgccaa gcgaagcaaa
    13921 gtaagagccc gatcgggggg tatcgccaat gcacggcctt cctggctgta tgggtgttat
    13981 tcggttttcc catgtccgcg tcggaccaga tgacgcctct tgaccgctac atccaggagg
    14041 cccagcgaga ggcggcgcac gtagcgccgt ccggcggctc tctctacgcg ccgcacgggc
    14101 ctctggccga actggtcggc gatgcgcgag cgcgccaagt tgatgacatg gttaccatcg
    14161 tggtcagcga aacggcgtcg gcctcgaatt ccggcaccac ctccagctcc cgcaagacca
    14221 gcgccagcgc cggggtcccg gccatctacg ggcacgtttc gaatcagctt tccaatctcg
    14281 ccagcgccag cagcgcgagc tctctggccg gcacgggaac gaccagccgg cagagcgcta
    14341 tctcgacgcg gctgagcgcc agagtcacgc atgtgcttcc caacggctat ctcgttgtcg
    14401 aggggagcaa ggagattacc gtaaattcgg accggcagct aattacggtg cgcggcatcg
    14461 tccgccccat cgacttggcc cgggataatt cggtcacctc cgaccgcgtg gccgatattc
    14521 agatcttggt agacggcaaa ggggtcgtga atgacgccat tcgccggccc aatttcttat
    14581 accggctgct gctgggtttg ctgccgttct agaaggacgg tgtttgatgc agacagaaag
    14641 cgcgggaacg cgcaggcggg atccgactaa aaagctgata gcagggctgc tcgcggcgat
    14701 ctccagcttg ccggcggctc gtttgggtga tcttactacc gtggaaggcg tgcgcgacaa
    14761 catgctcatc ggctacggat tggtggccgg cctgaagggc acaggcgacc gccagcagac
    14821 gattttcccc attcagactc tgaccaacat gctccagcgt atgggagtca cggtcaatcc
    14881 ggcccagatg caggtgcgga acattgcggc ggtgatggtg acggccaccc tgccgcctta
    14941 tgccgagccc ggcgcccggc tggatgtggt agtgagctcg atcggagacg ccgccagcct
    15001 gcaaggggga acgctgctgc tgacggctct caaagcgtcc accggggaca cctacgccgt
    15061 ggcccagggg ccgctgtcga tcggcggctt caccgcaggc acggcccaaa ccggcgtaca
    15121 ggtgaaccat tcgaccgtag gacgcattcc tggcggcgcg ctggtcgagc gggccgcgcc
    15181 caccgagcct cccggcaatt cgggtttaaa ccttcaattg cgccggcctg attttgcaac
    15241 cgctgcacgc atcgccgaag ccgtgaacaa gaaatttggc gccggcgttg cccgggctga
    15301 aaacgccgct gtggtccggg ttgccatgcc ggaagccttt cgctcgaagc ccgtggattt
    15361 cattgcggcc ctcgagccgc tcgaggttgc ggcggagcac ccggcccgcg tggtgctcaa
    15421 cgagcgcacg ggcaccgtgg tgctgggcaa ggacgtccgc atcgcccccg tggaggtgct
    15481 gcatgggaac ctttccgtcc agatcgtcac cacactcgag gtaagccagc cctcgccgct
    15541 ttctccgggt caaaccgcgg tcgtgcctca aaccggcgtc gctgtgaacg aggagaaggc
    15601 caggcgggtc gtcctgaaag aaggagctaa cgtagaggag ctcgtgcagt cgctcattgg
    15661 aatcggttcc acgccgcggg acgtcatcgc catcatccag agcatcgccg ctgccggcgc
    15721 cctggacgca gtcgtggagg tgatctaggt ggctggccaa atcgaatcgg ccgtctggag
    15781 ccgtattcgg atgctgccca cacaacaaca gcagatagcc gaagcatccc gccagttcga
    15841 ggcgctgctg atagagcgcc tgctgcaaat gatgcgcccg gccacacaag aagatcagca
    15901 gggagcgggc agagcgccgg aaacctatat ggaaatggcc gagcaagcga tggcgcaggc
    15961 cctcgccgag cggggcggcc tgggcatagc gcggctgctc cagcgcggac tggaagggcc
    16021 gaagggtaca gatcggaaga gttcggccga taagcagaat gaggccaaga gtggccggcc
    16081 tgtgcggtag gccgcgtggc cgcaagagaa ccgccgagca aagatgcata accaattgca
    16141 gggctggcct cccgcccggc gcgtggccgc tctaagacag gttggctatg agaattgacg
    16201 acaatttttc tccttcaagc gcttccggtt ccaacgccgt tcgatcgccc agcatcagcc
    16261 aagcgcctga ggtgcgcagc tcgggccgcc ctcaaagcgc cgggttggat ggcgccaccg
    16321 accgcgcgag tctctccgcg ctcggagtcc atatttcacg ggctctcgaa caagatccgc
    16381 cggaaatcgt ggccaagata ggccagttgc agcaggccgt ggcgaacgga acgtatagcg
    16441 ttccgtccag cgccgtgagc gtgaaaattg ttgcttctgc actcggttct ttctgatgag
    16501 acggatcaat aaagagcggg cgggagcgga agtatggaag taaccgctag cgaggataca
    16561 gccgccctcg ttgaaatcca gtctcgtctg gaagccttgg ctgccgcatt ggcttccggg
    16621 aagtccgacg acgtggagca gcgggccggg cagctcgaca gcgcgctgaa gcctttggaa
    16681 aagcttcgcg agacgttttc cggaaaagat aaaactcccg gaagcgctgc cggtctcgga
    16741 ccggcgctgc atcgaatccg cgcccaatgc gccgccctca gggaagcagc ggcgcgcggc
    16801 gaagccgccg tctgcgcgct ggcgggaatt ctggctttgc tatacaacgc cgaggctggc
    16861 gctcaatact cggcgcgggg ctcgccgagt ctgccctcgg cgccccggct ccgggcggaa
    16921 gcttgagcgc gaatgcaacg agtggaagag cgcggccttg gcgggcaagc aggccgctga
    16981 gccttttgac atcgctgcct ctcaggccag cccggcgccg gtaacggttc ggcacatcgc
    17041 cagaccggcc aaaaaaggca aggcgtattc ggattcggcc gaagatccgg ctggggatcg
    17101 aacatggcta acattctcgc ttcactcggc gcggcggcag gcaacctggg agctttcggg
    17161 caggccctgg cggtcactca gaacaacgtc ggaaacgcca cgacgcccgg ctacgcccgc
    17221 caaaggatca acttcgaagc gctccctttc gatttattgg gaggcgaatc aggcggcgta
    17281 accgttcaga gcgtccaaag cattcgtgac cagtttctcg atcagcaagt aattactgct
    17341 atacagaacc aatcgtattt tcagaatctg gcgcaagcgc tgacgcagat agagccaagc
    17401 ttccagctct cgggcaacct gtccattggc gcgaccctgg accagttttt taactcgttc
    17461 tccgcgctat cgacttcgcc caacgatttc actctgcggc agcaagtgct ccaatcggct
    17521 cagaccctgg cgacggccat tcactcgagc tataacggcc tgaccagcga gcgcgcagcg
    17581 ctcgaccagc aggcaggcgt tttagcaaat cagatcaaca gcctcacggc cgaggtggct
    17641 catttgaacg ccgcacggtc tacggccgac gcgaatgaag acaacagcgc caatcagacc
    17701 cgccttagtc aggtgctgga gcaactggcc gggctggtgg actttcaaac ggtgcgccag
    17761 aacgacggct ccctttccct ggtattgcgc ggcggcgcgc cgctggtggc gggaacggcg
    17821 tcgttcaact tgcaggcggt tcctacggcc tcgcggctcg agatccgtga cgccctcggc
    17881 aacgatgtgt cgcccagcgt gacaggcggg caactgggag cggtcctgaa cttacggaac
    17941 acgaagattc ccgcctacct gggccagctc aatcagcttg cggcgacact cgccgatgca
    18001 gtgaacaacc aattggcgca aggccgtgat ctggccggcg taccgggtaa accgatcttc
    18061 cagtacacgt cgctggcatt tacagggacc ggccgaaccc cggggaccgc cggcgccgct
    18121 acgccttctc cccccgattc tgtgacggtc agcttctccg ggggactaag cggctcgatc
    18181 acggcagacc tgggcagctt ttttgtagcg gcggcgccgc ccgcgggcct cgctgccggc
    18241 gacacggtca ccgtgaactt cacgagcgca gacggagcca caagagcctc gatcaccaca
    18301 acgccgctct cggcggggga cgccacagcc accatcgccg cgcgcctcaa tgaccaaatc
    18361 gcgttgagcc ctgcgctggc cggcaagttt agcttcagtg atgcgggggg acgcctgaag
    18421 ctgatggaaa gcgacactgc tgggcagggc ttcacgttca ctgcttcgac caatgacccg
    18481 ggcttcatca ccgggctgga ggccgggggc acagtcggcg gagaatccgc ccaggagatc
    18541 gccggcgccc tcaacgctca ggtcgctgcc gacccggcgc tttcggcggc aggcgtgcgc
    18601 ttcacagttt caaacggtga ggttcgcctc gatgccggcg tcagcttcac ggccaccgtc
    18661 acggataatt cgaacggtac cggctttgct tcgggcctgg cgggaacgtt cactgccgga
    18721 ggatcgcccg cggcggcaac cttcgccgcc acaggactcg ggaattccga gatcgcggcc
    18781 tcggggccta attcttcggg cggcaacgaa aacgcgctgg ccctggcgca attgggatcc
    18841 tcgccgattg tggccgggtt cacacccagc cagttctatg gaaggctggt ttcgcgagtg
    18901 ggcgaagaca gcagcgaggc ccagaccagt ctggagactg aaaaccagct tttgacgcag
    18961 gcgcagaacc tgcgcgattc gttttccggc gtctccctcg acgaggaagc gacccagtta
    19021 actcagtttc agaaagcgta tgaggcaacg gcgcgggtga tcacggtgct cgacagcctc
    19081 gcggatacgg tcatcaattt gataggcagc cccttgacat gattttccca cgagtcacat
    19141 tgacgatgct gagccggcag tcggtcatcg acctgcaaac gatcgaagcg cgccggcagc
    19201 atctcacggc agaagtttcg tcgggtctga gagtcagaaa tccatccgat gatcccgccg
    19261 gggcggcggc catcatccag accaacagcg actttgaggc caacgcccag ctcttgaaga
    19321 acctgcaagg cttgggcgac cagttgcaga gcgtggatgg ggcgctcaat caggccatta
    19381 cgctcgtgca aagggcgcaa agcctcgcct cagaagctgc gaacttcaac caaacggcag
    19441 aaactcgatc ttcgatcgcc accgaggttg ccgggctgat tcaaaacctg atcaccatcg
    19501 cgaacacgag tttcgggggc aagtacgttt ttgccggtgg gcaggatggg gtgctgccgt
    19561 tcgtcgccga tccgagcagc acaaacggcg tgatctatcg cggaggaacc gggcagcgct
    19621 cgatcgcgtt tcccggaggg gctcagtcgc cggtgagcgc cgatggacag agcatatttt
    19681 tgaatcccag cgatttcacg ggcagcggac gcacggctgg cattaccggc gcggcgacgc
    19741 caactccgcc ggtggggatc ggggtcagtt tctccaacgg cgtgacgggg gcaatcaccg
    19801 ccgatcttcc aagtttcttt gtcgctgccg cgccgccgag tgtccccgca gccggagatc
    19861 agatcacggt tcacttcatt tcagccgacg gcgcgatcaa cagttccatt acagctacac
    19921 tcggcggcgg tgaaactgcg gcgcagatcg tctccgcgct caatgcacaa atcgcggtca
    19981 cgcctgcgct cgccggcaag atcaccttca gcgaccagag cggcaacttg aagatcgtgg
    20041 aaagcgatac ggccggccag agtttcacct ttaccgccac cgacactggc acggtgacga
    20101 gcggcctcga gggcggcggc gttatcgggg gcgaatcagc ccaggagatc gccgccgcct
    20161 tgaacgccca agtagccttg aacccggaac tgagcgcagc gaagatcaac ttctcggcgg
    20221 caaacgggga gatcaaggta accagcgccg aaagcctgac cttcactgcc gtggacttcg
    20281 atcgcggcac gggttttgtc tccggcatgg ccggaactca cagcatcggc ggctccaaca
    20341 gcgccaatgt gttccaggta ttgaccgctc tcgagaccgc cctgagggcg aatgacgtga
    20401 gcggtattca ggccactttg gatgatttgc aagccgcggt
//
LOCUS       DASREK010000121        12882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_735042, whole genome shotgun sequence.
ACCESSION   DASREK010000121 DASREK010000000
VERSION     DASREK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735042"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFA54_06050"
     CDS             <1..945
                     /locus_tag="VFA54_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50402.1"
                     /translation="RRAAGPQIAELYETRFRGTPVFFISDDHDYFENDEADDRLVTFP
                     PDDFMLRMARATRRLYYPEFLPDPNRPPGLPGSSPLDRPGGVSECFGTLRYGRLVEIL
                     MYDCRRFLTLTGPSAVFVPPETEEWLTGRMKDTAVTHVVNVPSTPPGWSAGKFGEWYP
                     DILDNQGRLGTSRPKPYWQPGWAAQHDRLLQACSAMQQRIPLFISGDLHAIGEGRIRR
                     TGSADLRANPVISILSGPIGTSTDGWPSASRGTPALPPSRIEIEEMQRPLEENGFLVV
                     DFTPDQITARFFKWNVNMPDDVMDRLEPFRTTRMSRTG"
     gene            1009..1845
                     /locus_tag="VFA54_06055"
     CDS             1009..1845
                     /locus_tag="VFA54_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZS50403.1"
                     /translation="MTRRTWLAALAAASAAAPQELKPTPRKGRLKQCVTRGVFGRNMP
                     FDEMCRHAARLGVVGFDLIGPEDFPVLKKYGLIPTMVPGAMTLTGGFNHKEDHPAVEK
                     ALRELIDNAAAAGAPNVIALSGNRRGISDQEGMDNVVALLSRVKGQAEDRGVNVCLEL
                     LNSKVNHPDYQCDHTAWGVEVCRRVNSPRVKLLFDIYHMQIMEGDIIRTIRGNFQWIA
                     HFHTAGNPGRHEMDDNQEMNYRGIARAIADLGFQGYIAHEYSPLRDPIQSLEETLAIF
                     TV"
     gene            complement(2087..3271)
                     /gene="hpnI"
                     /locus_tag="VFA54_06060"
     CDS             complement(2087..3271)
                     /gene="hpnI"
                     /locus_tag="VFA54_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HZS50404.1"
                     /translation="MTVLCGAVYALCLLVAAGGFIFYGLTLIAARRRRVEAAVTKRFT
                     PPITILKPLAGLEPDLEANLRTFFKQDYPEYQILFGVRSSDDAAASVAQKLIAQHPGR
                     DASLVIAGDALYPNAKVSSLSRMMERARHEILIISDSDIRATPGYLQGIAADFADPQV
                     GVSFCPYRAEPAPSWTPQNTRGSGPTFWSLLEALTLNTEFWCGVLVARMLEGIRFAVG
                     PTMALRRKYLEELGGFAAVGDYLAEDFVLGQWAQRYGYRAVLSRHIVDHCIGSQPFWP
                     NLQHRVRWARSTRRSRPWGYVGQIFTNPLPFALILLATRAAPLGLLVVAARAAVAAAI
                     AGGLLRDRLARRYWWLIPLADVASLVVWILGFFGNTVQWRGRRYRLFRDGRFQPCNLP
                     EA"
     gene            complement(3326..4801)
                     /locus_tag="VFA54_06065"
     CDS             complement(3326..4801)
                     /locus_tag="VFA54_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50405.1"
                     /translation="MPSDDTQLMAVDRAVLAGYWSRLLTPQDVHVYPLFRLIRLYFEL
                     HFLERYAWLQSVAIAAHLASVILLFLLCRRFFHSSWAALLAALLFAWHALGRVAFLRK
                     ADEPYILSLPFLLLAVYCLQRLRTSPQPAWAAGCLLCLLAAIGLHSMGAMLAIPGVLL
                     AYYAPRPQGPDAKLGARRCRIAAWLACLLPALAGACLWAAAWPPLARSRPAGHRPIPH
                     NLGDFFTWLLDASRGTLWHFAFLARRTHPTPAAVAIAGIAFAAVLVAVRRQPSFRWIL
                     TVAALAIPPVFVTLFVRSSNDYGTSRYFYQSSLLAAVAAAAAFDLLLAALAQRPALRL
                     SAIAIILAAAPFYFMSQEKLVEKTVRELQSGPASTREFWAAWAGFFNRAAAGRGSPPF
                     RLPHVLLGSSIYLDEVFNMCNPRGKAGILVLPAARTHAADCMAFWERAKQYGAGSLSV
                     HSGAWTPSMLVAGFPQAAPETGAWVCNAWADEGPSQPRMLH"
     gene            complement(5118..5501)
                     /locus_tag="VFA54_06070"
     CDS             complement(5118..5501)
                     /locus_tag="VFA54_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZS50406.1"
                     /translation="MRTTLLHLAAGALLFGAGLLIGQRTAAPRKTLLHVFAFKAVEGA
                     TPQRLDDLWSATRKLASQEPAIQNIWMGKIVRHQRDWQYAVVMEFENEAGHKKYEAGP
                     AHAEWLKTYSQIRAEPTFTLDLQGQ"
     gene            complement(5658..6263)
                     /locus_tag="VFA54_06075"
     CDS             complement(5658..6263)
                     /locus_tag="VFA54_06075"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZS50407.1"
                     /translation="MRRAVYAGVLGLALIVFRQESQLAAFPVEEGADRIGAAAPRLAL
                     ERWVNSSPLEIANLRGRVVLIRWWTDTCPFCAATAPALQKLQTKYGGLGLQVIGVFHP
                     KPAGDWSVERVREGAKRLNMNFPVALDADWKALHRWWLDQRERSATSVSFVIDKRGII
                     RYVHPGVEFHEAAAGEGPGHDACHAQFHDIEQTIIRLLAEP"
     gene            6701..6961
                     /locus_tag="VFA54_06080"
     CDS             6701..6961
                     /locus_tag="VFA54_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50408.1"
                     /translation="MAPVIPLTAPRTRKSIDNSDQLAAFQVIHLAGARLVQDQNRELA
                     IEVPAENVDEEFVWALEKLGLCGLPITERDYPLEMGPSTSHE"
     gene            complement(7029..7946)
                     /locus_tag="VFA54_06085"
     CDS             complement(7029..7946)
                     /locus_tag="VFA54_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZS50409.1"
                     /translation="MKRPATQKSGSSLGCIMVAFWALLKEAFWKWQDDQAGRLAASLA
                     YYTLMSLAPLVVLAIAIAGLMFGQKAVEGQIVGQIKSLVGQSGAVVIQEIIRRAHQPR
                     AGILAGTLGFLFLLVGASGVFSELKDALNMVWGVPQATGGGVRGFLKRRLLPFAMTVG
                     VGFLLLISLVLNAVLDASVKYLNSLLPVAPWLLRMANFVLSFAVITLLFAIIYDILPD
                     VKIPWSDVWIGAATTSLLFTMGKTLIGFYLGTASVGSAYGAAGSLIVFLVWLYYSSQI
                     LYFGAEFTRIYSQRRGSAARAQAKLKDAA"
     gene            8078..11029
                     /locus_tag="VFA54_06090"
     CDS             8078..11029
                     /locus_tag="VFA54_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZS50410.1"
                     /translation="MPRQNLRIGLVLLSSALFCAAADLPVARNFEGQRIASIRFDPEN
                     QPLAQDEIQRALSLRPGMPFQVAHLRSTIKQLYATGRYERIEADGEPTAGGVAVVFRT
                     TNRWFISRVESDRAIKQPPTPGQVTAVSRLELGQPFHEDQLQEASQRVLNLLKRNGYY
                     EARVEPVLSRDPAHQEVNIRFAVEPGKRARFQTPVIVGEPEMPAGAVSAATKWKRFWF
                     LGWKPVTSETVQSGIRNVRKKYEKEDRLMASVSLEKMAYDSATRRVTPTLKVIGGPKV
                     ELRTEGAKISGRKKRDYIPVYDVGAVDRDLLVQGVRNLRDYFQIQGYFDVRVSYEENQ
                     IAADHEVITYTIRLGPRRKLEKVEIQGNRYFDTATIAERMLIQPAGLLRLRHGRYSQA
                     LARRDESAIKALYNSNGFREANVEFETIDNFQGDPDQMAVIVKIEEGPQYLVSEFSIS
                     GIRQLNEQEILSHISSVAGEPFSETNVASDRNYIIQRYHDAGFPDASFVWSTKPGPRP
                     NTVSVHYAVTEGVRRYVRDVLLSGMDTTSPRLVAPALHIQKGDPLSLPAMSATQENIY
                     NLGVFDQVNMAVQNPDGNTERKYVLFQVQEGRRYQLATGFGAEVARIGGGQNSLANPA
                     GATGFTPRGSVDLSRLNMWGLGHTLNFKGRVSTLDRLASLNYYAPRYRNVEGRNITVT
                     GLWERSLNVRTFSAHRLEGSTQLSQKLSKASTMLYRFTYRRVSVDASTLKIEPLLIPL
                     LSQPVRIGILSANYVLDRRDNSADAHSGIYNTVDLGMAANAFGSKRNFGKLLETNSTY
                     HPLGKNLVLAQRLEVGWLGPFALQTGLTDAQAIPLPERFFGGGGTSHRGFPDNQAGPR
                     DPLTGFPLGGNALLFHNTELRFPFVGDNVSGVLFHDMGNIYSTISKISFRIHQRDMAD
                     FNYMVHAVGFGIRYRTPVGPVRVDLAYSINPPAFMGLKGTPEELLFGTAPRVPQRINH
                     FQFFFSIGQAF"
     gene            11041..11664
                     /locus_tag="VFA54_06095"
     CDS             11041..11664
                     /locus_tag="VFA54_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50411.1"
                     /translation="MLLLLGLATAASAAVVLDRIAVTVENEVITEGELLDDIRLTAFL
                     NGDQPDFGPQSRRQAAERLVDQYLIRREMKISRYQPDTGGAEKALSAFKRSRFQSDEQ
                     YRQALKSYGISEEELLDKLRWQTAALQFIDFRFGQGLPASPPPSRSGDHLEIEAPPEN
                     HEAPSAEPAVAGQGERGIPPAEEEVLDRWLKQARSTTRIEYNPEAFQ"
     gene            11661..>12882
                     /locus_tag="VFA54_06100"
     CDS             11661..>12882
                     /locus_tag="VFA54_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50412.1"
                     /translation="MSRIPKIALIVAGSIAGVVVIAAIGVLIILQTSWFRQFAASKIE
                     TELETATGGRSAIGGFSFSPRGLRVEVDNFVLHGTEGPDEPPLFRAQSIIVGLKILSV
                     FHHRLDIASLEVDAPQAYIRVARDGATTLPHPQALRPAGNKGPIQTVLDLAIGHFDLR
                     GGSIIFADRKMPLTARGENLRARLTYHALGPAYSGDLSMAPVYVQTGSQPALPVNVRI
                     PIRIESNQIVISNARLDAPSSHLQISASLADLAAPHGLLSLKGRLALDELAQSFDPVL
                     LQKVQNVPPLDLDFSSDLNRARLEAVKLEASLGESHLEASGATIDLRKLSGALAFRGD
                     LALAELGRVFDFSARPQGRVTISGDIKLAGANHYSVAAKVSARNVAFNAGKTRLAGVN
                     AAAQIEANPEAIYAR"
BASE COUNT         2701 a         3916 c         3927 g         2338 t
ORIGIN      
        1 cgccgggccg caggcccgca aatcgccgag ctctatgaaa cgcgctttcg cgggaccccg
       61 gtctttttca tcagcgacga tcacgattac ttcgagaacg acgaagccga cgaccggcta
      121 gtgacttttc ctcccgatga tttcatgctg cgaatggcgc gcgcgacgcg ccgcctgtat
      181 taccccgagt ttctgcccga cccgaatcgc ccgccaggat tgcccggttc atcgcctctt
      241 gaccgcccag gaggggtttc ggagtgcttc ggaacgttgc gctacggacg cctggtcgaa
      301 atcctcatgt atgactgccg ccgcttcctg actttgaccg gccccagcgc ggtcttcgtg
      361 ccgccggaaa ccgaagagtg gctgaccggc cgcatgaagg atactgccgt gacacatgta
      421 gtcaatgtgc cctctacacc gcctggttgg agcgccggga aattcggcga gtggtatccg
      481 gacatactgg acaaccaggg caggctgggc acgtcccggc caaagccgta ttggcagccc
      541 ggctgggcgg cgcaacacga ccgcttgctc caggcgtgct cggccatgca gcaacgaatc
      601 ccgctgttca tcagcggaga tttgcatgcc attggagaag ggcgcattcg gcgcaccggt
      661 tcggccgacc tccgggccaa tcccgtgatc tccatacttt ccggtccgat cggcacgagc
      721 actgacggct ggccctccgc gtcgcgcggc accccggcgc tgccgcccag ccgcatcgaa
      781 atcgaggaaa tgcagcggcc gctggaggag aatgggtttc ttgtcgtgga tttcacaccg
      841 gatcagatca cagcgcgatt tttcaaatgg aacgttaaca tgccggatga cgtgatggat
      901 cggctggaac cctttcgtac aacgcgcatg tcacggaccg gctgatcagc gcgggctcga
      961 attgcccggc tgaagaaata ggccggtgca gcctggggga gaagtgaaat gacgcgaaga
     1021 acatggctgg cggcgctggc ggccgcatcg gcggccgctc cgcaggaact caagccaacc
     1081 ccccggaagg ggcgattgaa acagtgcgtc acgcgcggcg ttttcggcag aaacatgccg
     1141 ttcgacgaaa tgtgccggca cgcggcgcgt ctcggggtgg tcggcttcga tctgatcggg
     1201 cccgaggatt ttccggtttt gaagaaatat ggcctcatac cgacgatggt ccccggagcg
     1261 atgacgctca ccggcggctt caatcacaaa gaggatcatc cggcggtaga aaaggcgctg
     1321 agagagctta tcgacaacgc ggcggctgct ggagctccca acgtgatcgc tctctcgggg
     1381 aaccggcgcg gcatttcgga ccaggaaggc atggacaacg tcgtcgccct attgagccgc
     1441 gtaaaagggc aggccgaaga cagaggcgtc aacgtctgcc tggaattact gaacagcaaa
     1501 gtgaatcacc cggattacca gtgcgaccac acggcttggg gtgtggaggt ctgccggcgt
     1561 gtaaactcgc cgcgcgtcaa gctgctgttc gacatctacc acatgcagat catggaaggc
     1621 gacatcatcc gcacgatccg cggcaatttc caatggatcg cgcattttca cactgccggc
     1681 aatcccggac ggcatgagat ggatgacaat caggagatga attaccgggg aatcgcccgc
     1741 gccatcgcgg acctgggatt tcagggctat atcgcgcacg aatattcgcc gctccgcgat
     1801 ccgattcagt cgctggaaga aactctggcg attttcaccg tctaaacggc cggtcgcgca
     1861 cgagcgcgcc gatctggccg cgcggcaaga atcctaacag cagattcttc gctccgcttg
     1921 aaaaacaaag gaaacactcg taccagcagc tcgccagcga cgccggacag cgtcactgcg
     1981 cgctgaccag ttccgcttcc cgcgccgtat tcgcagagag tttttccaat cgggcttctt
     2041 aagaaaatcc acaataggcg gcccttttgc gatgcaatgc cggccatcag gcctcgggaa
     2101 ggttgcacgg ctggaaccgg ccatctcgaa acaatcgata gcgcctgccc cgccactgca
     2161 cggtgttgcc aaaaaatccg agaatccaga ctaccagact tgccacatcg gccagcggga
     2221 tcagccacca gtaccgccgc gcgagccggt cgcgcagcag cccgcctgca atggcggctg
     2281 ccacggccgc cctcgcggcg accaccagta atccgagcgg cgcggcgcga gttgcgagca
     2341 gaatgagagc aaaggggagc gggttggtaa agatttggcc cacatagccc caaggccggg
     2401 accggcgggt ggagcgggcc caccgcacgc gatgttggag attcggccaa aacggctgcg
     2461 agccgatgca atggtctaca atgtggcgcg agaggacggc cctgtacccg tatcgttggg
     2521 cccactgccc caacacaaaa tcctcggcca gatagtcgcc cacagcggca aagccaccga
     2581 gctcctcgag atacttgcgg cgcagcgcca tcgtgggacc tacggcgaac cggatgcctt
     2641 cgagcatccg cgcgaccaag acgccgcacc agaattctgt attcagcgtc agggcttcga
     2701 gtaacgacca gaacgtggga ccagagcctc gtgtattctg gggcgtccac gaaggagccg
     2761 gttccgcccg gtagggacag aacgaaacgc ccacctgcgg gtcggcgaag tccgcggcga
     2821 tgccctgcaa gtagccgggt gtggccctga tgtcgctatc gctgatgatc aggatttcgt
     2881 ggcgcgcccg ctccatcatg cggctcaggc tggagacctt ggcgttgggg taaagcgcat
     2941 cgccggcgat caccaggctg gcgtcccggc cgggatgctg cgcaatcagt ttttgagcga
     3001 ctgacgccgc cgcgtcgtcg gaagatctga cgccgaacag tatctggtat tcgggataat
     3061 cttgtttaaa aaacgtacgg aggtttgctt cgaggtccgg ttccaggccg gcgagcggct
     3121 tgaggatagt gatgggcggc gtgaatcttt ttgtgacggc ggcctctaca cggcgcctgc
     3181 gggcggcgat cagggtgagc ccgtaaaaaa taaagccgcc cgcggcgacc agcaagcaga
     3241 gggcatacac ggccccgcac agaaccgtca ttggaaagaa gcagggccgc tgaaggcccg
     3301 aaggaaaaag gctgcgataa cccaatcagt gtagcatgcg gggctgcgat ggcccctcat
     3361 cggcccaggc gttgcagacc caagctccgg tctccggcgc agcctgcggg aaacccgcca
     3421 caagcatcga cggagtccag gcgcccgagt gcacggaaag cgagccggcg ccatattgct
     3481 tcgccctttc ccaaaaggcc atacagtcgg ccgcatgagt gcgggccgcg ggaagaacca
     3541 gaatgcccgc cttaccccgg ggattgcaca tattgaacac ctcgtccaga taaatcgacg
     3601 aaccgagcag cacatggggc agccggaatg ggggcgagcc tcggccagcg gcggcccggt
     3661 tgaagaatcc agcccatgca gcccaaaatt cacgagtgga agcaggaccc gattgcagct
     3721 cacgcacggt tttctcaacc agtttttcct ggctcatgaa ataaaagggc gcggccgcca
     3781 gaattattgc gatggccgac agccttagag cagggcgctg ggcaagcgct gccagcaaca
     3841 ggtcgaaggc agcggccgcc gccacagcgg ctagcagcga gctctgataa aaataacgcg
     3901 atgttccgta gtcattcgag ctgcgcacga acagggtgac gaaaactgga gggatggcca
     3961 gagcggccac ggtcaaaatc caacggaagc ttggctggcg ccgaacagca acaagaactg
     4021 cggcgaaagc aattccagca atcgccacag ccgcaggcgt agggtgcgtg cggcgcgcga
     4081 gaaaagcaaa atgccaaagc gtcccccgcg aagcatccag gagccaggtg aaaaaatcgc
     4141 caaggttgtg agggatcggc cggtggcctg ccggacggct gcgggccaga ggcggccagg
     4201 cggcggccca aaggcacgct ccggcgagcg ccggaagcag gcaggccagc caggcggcga
     4261 tccggcagcg ccgcgcgccg agctttgcat caggtccctg tggtcggggc gcatagtaag
     4321 cgagcaaaac cccgggaatg gccagcatgg cccccatgga gtgaaggccg atggccgcca
     4381 gcaggcacag aaggcacccc gccgcccatg cgggctgcgg cgaggtccgc aagcgctgca
     4441 agcagtacac tgccagcaaa agaaaaggca ggctgaggat gtaaggctcg tcagccttgc
     4501 gcagaaaggc gacccggccc agtgcgtgcc aggcgaacag gagggcggcc agcagcgccg
     4561 cccagctcga atgaaagaag cgccggcaaa gcaaaaacaa caaaatcacg ctggccagat
     4621 gggcggcgat ggccacggac tgcaaccagg cgtaccgttc caggaaatgc aactcgaagt
     4681 acaggcgaat taagcggaaa agaggataaa catggacgtc ttgcggagtg agcaggcggc
     4741 tccaatatcc ggccagcacc gcgcggtcga cagccatcaa ctgagtatca tcagaaggca
     4801 tcaccagcaa aaaatcggag ggcggccacc acaatctgac agatgcggct aatacagcga
     4861 agagcaaaag gccaattgta agatttgccg cccgggaggc gccggtcatt gccaatacct
     4921 ggcgttccgg tgccggccct tccggatcag gcttaccgca aaaagagatc ttcctaggcg
     4981 catgagattc accaggcgca aaggcgcccc gccgcgcggc tgcgccgcga gccggatgca
     5041 gccggattcg caaatgcccg gccagctcgc gcagggccgc atgggggcgc accgccgccg
     5101 agacggcgcg cccgggacta ctggccttgg agatccagcg tgaaggtagg ttcggcgcgg
     5161 atctgggagt acgtcttcag ccattccgcg tgggccggcc cggcctcgta cttcttgtga
     5221 ccggcttcgt tctcgaactc cattaccacg gcgtactgcc aatcgcgctg gtgccggaca
     5281 atcttgccca tccagatatt ctggatggcc ggctcctggc tggccagctt gcgagtcgcc
     5341 gaccagagat cgtcgaggcg ctggggagtt gctccctcca cggccttgaa cgcgaacacg
     5401 tgaagcaggg ttttgcgggg cgcggcggtc ctttgcccaa tgagaagtcc ggctccaaat
     5461 aggagcgctc cggccgccaa atgcagcagg gtagtgcgca tcgaacctcc tggaagactc
     5521 gccgctagcg aaggcttgca gcggcgaatg aactttgagt ttaccagttt tggctgttcg
     5581 cgccggaggt ccctgtgccc agctctcttg gttgaattct tgctcggact cgtagcgcgt
     5641 cacggcgcat gcgtgcctta aggttccgcg agcaaacgta tgatcgtttg ttcgatgtcg
     5701 tgaaactgcg cgtggcatgc gtcgtggccg ggcccctccc cggctgctgc ttcatggaac
     5761 tcgacgccgg gatgaacgta tcgaatgatg ccgcgcttgt cgattacgaa cgacacagag
     5821 gtcgcgctgc gctccctttg gtccagccac cagcggtgca aagccttcca gtctgcatcc
     5881 agggcgaccg gaaagttcat gttcaggcgc ttggctcctt cgcgcacccg ctcgacgctc
     5941 cagtcgcccg cgggcttggg atggaagacg ccgattactt gcaaacccag gccgccatat
     6001 tttgtttgca gcttttgaag cgcgggcgcc gttgccgcgc agaacgggca ggtgtcggtc
     6061 caccagcgaa tcaagacgac tctgccgcgc aggtttgcga tttcaagggg cgaggagttc
     6121 acccagcgct ccagcgcgag ccgcggagcg gcggcgccaa tacggtcggc cccttcctcc
     6181 accgggaagg cggcgagctg cgactcttgc ctgaaaacaa tcaaggctag tcccaacact
     6241 cctgcataaa cagcacgcct cattggccgt tcgccttgaa gagaggatac cattcttcgc
     6301 tgccggaatt tccgtgcggg ctcggtcaat tccaggcgcg gctgactgct gccgggtatc
     6361 gccgcgcgtt tttcggagcc tatccctacc gcggtctatc cggaatcctg cataccggct
     6421 cgagccggtt tgtgacccgc tcttatcgag ttccgaatca gggcctgctt cgcatgctat
     6481 ccggcttcga acctggaggt tgtggccgct ctgttggggt ttgaacggtt gtacgaatcg
     6541 taacaaaagg tgcttttttg gattttgaca aggctgcgga ctgggtaaat ttgccatgcg
     6601 tatcaatgaa aggaatgcaa agtgctcgga tgcaaatggc ttttggcgtg caacaaaatc
     6661 gaagagagaa cccgcttcga attcaggagg gggttgagat atggcgcctg taatcccgct
     6721 caccgcaccg cgcacccgaa agtcaatcga caacagcgac cagcttgcgg cctttcaagt
     6781 gattcatctg gcgggcgctc ggctggtcca ggatcaaaat cgagagctgg ccatcgaagt
     6841 ccccgccgaa aacgtggatg aagagtttgt ttgggcgttg gagaaactcg ggctttgcgg
     6901 gttgcccatc accgagaggg actatcctct ggaaatgggg ccttcgactt cacacgagta
     6961 gcccagccat aactttcgct gcggtctttc gagtcgcggc tgctccaaca caacgcaggg
     7021 catctttctc aggccgcatc cttgagcttg gcctgtgcgc gggccgccga accacgccgc
     7081 tgggagtaaa ttcgggtgaa ctcagcgccg aagtaaagaa tctgcgaaga ataatacagc
     7141 cagacgagga atacgatgag tgatccagcc gccccgtagg cggatcctac gctcgccgtg
     7201 cctaggtaaa acccaataag cgttttgccc attgtgaaca gcagggaagt agttgcggcc
     7261 ccgatccaga cgtcgctcca gggtatcttg acatcgggca ggatgtcgta aatgatcgcg
     7321 aataacagtg tgataacggc aaacgacaga acaaaattcg ccatgcgcaa cagccatggg
     7381 gccaccggca gaagactgtt gagatacttg accgaagcat ccagcacggc attgagcacc
     7441 agcgagatca gaagcagaaa cccgacgccg accgtcatgg caaagggcag cagccttcgt
     7501 tttagaaacc cccgcacgcc gccgcccgtt gcttgcggaa caccccagac catgttgagc
     7561 gcgtccttaa gctcagagaa aacacctgac gcgccgacca ggagaaagag gaatccgagg
     7621 gtccccgcca ggatgccagc cctgggttgg tgggcccgcc ggatgatctc ttgtatgacg
     7681 accgctccgg attgtcccac caggctcttg atctggccga cgatttggcc ctccacggcc
     7741 ttctggccaa acatcaagcc ggcgattgca attgccagca ctaccagcgg cgccagcgac
     7801 atgagggtgt agtaggccag ggaggccgcg agccgcccgg cttgatcgtc ctgccacttc
     7861 cagaatgcct ctttgagaag agcccagaag gcgaccatga tgcacccgag ggaactgccc
     7921 gacttttggg ttgccggacg cttcatctgc tttgcaaaag tattgcctgg cccccggcgg
     7981 ctccgtgcgc tacacgtctg cggcagcact gactggaccg ccatctgggc ctacgggctg
     8041 cggttccgct ggcatcaaag ttgcgggctt tatcaatatg cctcggcaga accttcggat
     8101 cgggttggtt ctcctgagct ccgccctctt ctgtgctgcc gccgatcttc ccgtggcgcg
     8161 aaatttcgaa gggcagcgaa tcgcctcgat ccggtttgac ccggagaatc agcccctggc
     8221 gcaagacgag atccagagag cgctctcgct caggccgggc atgcccttcc aggtggctca
     8281 cctccgctcc accatcaagc aactttacgc gaccggcaga tatgaacgga ttgaggcgga
     8341 cggcgagccg actgcgggcg gcgtcgccgt ggtctttcgc acgaccaacc ggtggttcat
     8401 cagcagggtt gaatcggaca gggcgatcaa acagcctccc acgcccggac aggtcaccgc
     8461 ggtgagccgg ctcgaactag gccagccctt ccatgaagat caattgcagg aagccagcca
     8521 gagggttctc aacttgctga agcggaatgg gtattacgaa gccagagtgg agccggtgtt
     8581 gagccgcgac ccggcgcatc aggaagtcaa tatccgcttc gctgtcgagc ccggcaagcg
     8641 cgcgcggttt cagactccgg tcatcgtcgg ggagccggag atgccggccg gcgctgtcag
     8701 tgctgctacc aagtggaaac ggttttggtt tttgggctgg aagcccgtga ctagcgagac
     8761 tgttcagagc ggcattcgga acgtacgcaa gaagtacgag aaggaagacc ggctgatggc
     8821 ctcggtttct ctcgagaaaa tggcctacga cagcgcgacg cgccgggtga ccccgaccct
     8881 caaggttatc ggcgggccga aagtggagct ccggaccgag ggcgccaaga tatccggcag
     8941 aaagaagcgg gactacattc ccgtctatga tgtcggagca gtagaccgcg atttgctcgt
     9001 gcagggcgtg cgcaatctcc gggactactt ccagatccag ggttatttcg atgttcgggt
     9061 cagctacgag gaaaaccaaa tcgcggcgga tcatgaagtc atcacctata ccatccgcct
     9121 gggtccgcgc cgcaagctgg agaaggtgga gattcaagga aaccggtatt tcgataccgc
     9181 cacgatcgcg gaacggatgc tcatccagcc ggccggtttg cttcgcctgc ggcacggccg
     9241 gtacagccag gctctggccc ggcgcgacga gagcgcgatt aaagctttat ataacagcaa
     9301 cggcttccgg gaggccaacg ttgagttcga gacgatcgac aatttccaag gtgatccgga
     9361 tcaaatggcc gtcattgtga agatcgagga gggaccgcag tatcttgttt ccgaattctc
     9421 cataagcgga atccgccagt tgaatgagca ggaaatactt tcccatatct cctcagtggc
     9481 aggggagccg ttcagcgaga caaacgtcgc gtcggaccgg aattacatca tccagcgcta
     9541 tcacgatgcc ggatttccgg atgcgagctt tgtatggagc acgaaacccg gcccccggcc
     9601 gaatacggtg agcgttcact atgcggtgac cgagggcgtc cggcgctatg tgcgcgacgt
     9661 gctgctttcc gggatggata cgaccagccc ccgccttgtc gcgccggcgc ttcacatcca
     9721 gaaaggcgac cctctgtcgc tgccggcgat gagcgccacc caggagaata tttacaacct
     9781 cggcgtattc gatcaggtga acatggcggt gcagaatccg gatggaaaca cggagcgcaa
     9841 gtatgttctt ttccaggtcc aggaagggcg ccgctaccag cttgccaccg gcttcggcgc
     9901 ggaggtggcg cgaatcggag gcggccagaa cagcctagct aatccggcag gggcgactgg
     9961 cttcacaccg cgcggcagcg tcgatttaag ccgcttgaac atgtggggtc tcggccatac
    10021 gctgaatttc aagggccgcg tttctacgct cgaccgcctg gcctcgctga attactacgc
    10081 tcctcgctat cgaaatgtgg agggtcgcaa catcacggtg accggccttt gggagaggtc
    10141 gctgaacgtc cgaacctttt cggcccatcg cctggaaggc tcgacccagc tttcgcagaa
    10201 gctgtccaaa gccagcacca tgctgtaccg tttcacttac cggcgcgtca gcgtggacgc
    10261 ctcgacgctc aaaatcgagc cgctgctgat tcctttgctc tcccagccag ttcgaatcgg
    10321 cattctttcg gcgaactacg ttcttgaccg gcgcgacaat tccgccgatg cccattccgg
    10381 catttacaac acagtcgatc tcggaatggc tgccaacgcc ttcggctcca agagaaattt
    10441 cggcaagctg ctcgagacca attccaccta tcaccctctc ggcaaaaatc ttgttctggc
    10501 gcaaaggctg gaggtcggct ggctgggtcc gttcgcgttg cagaccggcc tcaccgatgc
    10561 gcaagcgatt ccccttccgg agcgattttt tggcgggggc ggcacttccc accgtgggtt
    10621 tcccgacaat caggcggggc cgcgcgatcc tctcaccggt tttccgctgg gcggcaacgc
    10681 tctgcttttt cacaacacgg agctgcggtt tccatttgtc ggggacaacg tcagcggcgt
    10741 gctgtttcac gatatgggaa atatctactc caccatcagc aagatttcgt tccgcattca
    10801 ccagcgtgac atggccgact tcaactacat ggtgcacgcc gtggggtttg gcatccgcta
    10861 ccggactccg gtcggtccgg tacgcgtgga cctggcgtac agcatcaatc cgccggcgtt
    10921 catggggctg aagggcaccc cggaagagct gctattcggg actgctccgc gggtgccgca
    10981 gcgcatcaac catttccagt tctttttttc catcgggcag gcattttgat gttgagaggc
    11041 atgcttcttc tcctcggtct cgcaacggcc gcctccgcag cggtggtttt ggaccggatt
    11101 gccgtcacgg tcgaaaatga agtcattacc gaaggggagc tgctcgatga cattcgtctg
    11161 acggcattcc tgaacggcga ccagcctgac ttcgggccgc agtcgcgccg ccaggccgcc
    11221 gaacggctcg ttgatcagta cttgatccgg cgggaaatga agatcagccg ttaccagccg
    11281 gataccggcg gagccgaaaa agccctgagc gcatttaaaa ggagccggtt tcagagcgac
    11341 gagcagtacc gccaggcttt gaagagttac ggaatcagcg aggaggagct gctggacaaa
    11401 ctgcgttggc aaaccgctgc tctccaattc atcgactttc gtttcgggca gggcctcccc
    11461 gcttcgccgc ctccatcccg gtccggagat caccttgaga tcgaggcgcc tccggagaac
    11521 catgaagccc cgtccgccga gccggccgta gccggccagg gagagcgagg catcccgcct
    11581 gccgaagaag aggtcctcga ccggtggctg aagcaggccc gcagcacaac acgcatcgaa
    11641 tacaacccgg aggcattcca atgagccgca ttccgaaaat cgccctgatc gtcgccggct
    11701 cgattgccgg ggtcgtggtg atcgccgcta taggcgtcct gatcatcctg caaacatcct
    11761 ggttccggca attcgcggca agcaagatcg agacggagct ggagacggcc accgggggcc
    11821 ggagcgcgat tggcggattt tccttctctc cgagaggact ccgcgtggag gtcgataact
    11881 tcgttctcca cgggacggaa ggtccggacg aacctcccct gtttcgcgcg caatcgatca
    11941 tcgtgggttt gaagatcctc tcggtgttcc atcaccgact cgacattgct tccctggaag
    12001 tcgacgcgcc gcaagcctac attcgtgtag cccgggacgg cgccacaacc ctcccgcatc
    12061 cccaagccct gcgccccgca ggcaacaagg gcccgataca gacggtcctg gatctcgcca
    12121 tcggccattt cgacctccgc ggcggctcca tcatctttgc agaccggaag atgccattga
    12181 cggctcgcgg agagaacctc cgcgcgcgat tgacctatca cgcgctcgga cctgcctatt
    12241 cgggcgatct atcgatggct ccggtgtatg tccagaccgg cagccagcct gcgcttccgg
    12301 taaacgttcg aatcccgatc cggatcgaga gcaatcaaat cgtaatctct aacgcccggc
    12361 tcgacgcgcc gtcttcccat cttcaaatct cagcctcact ggccgacctc gcagcacctc
    12421 acggcttgct cagtctgaaa ggccgccttg cgctggacga gttggcccag agcttcgacc
    12481 cggttcttct ccaaaaggtt caaaacgttc ctccgctcga cctcgacttt tcttcagacc
    12541 tgaatcgggc ccgcctggaa gcggtgaaat tggaagcctc cctcggcgag tcccatctgg
    12601 aggcctcggg ggcgacgatc gatctcagga aattgagcgg tgctctagcg ttccgcggcg
    12661 atctggcgct ggcggaactg gggcgcgtct tcgatttttc cgctcggccg caggggaggg
    12721 tgacgataag cggcgatata aagctggcgg gcgcaaatca ttattcggtt gcggcgaagg
    12781 tcagcgcgag gaacgtggcc ttcaatgccg gaaagacgcg cctggccggc gtgaacgccg
    12841 cggcgcagat cgaggcgaac ccggaagcga tctatgcccg cg
//
LOCUS       DASREK010000122         5185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_66059, whole genome shotgun sequence.
ACCESSION   DASREK010000122 DASREK010000000
VERSION     DASREK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66059"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..960)
                     /locus_tag="VFA54_06105"
     CDS             complement(34..960)
                     /locus_tag="VFA54_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZS50413.1"
                     /translation="MKLCTFEHHSKIYAGVVVRAKGGERRVLPVAEINARRGGDFPDD
                     LLALITAGRIEDLKNASVEDLHGAALPPAEIRLRLPYPAPPKIWCIGLNYLSHAEDLQ
                     AAQPEEPGSFMKPSSALFEPGGEILLPPAEVSSEVDAEGELGVVIGKECRDIPPELAP
                     EVILGYTTTLDMTALDVLRRNTRYLTRAKSFDTFFSFGPIITTADEIADVNAIEVITE
                     HNGSVFSRDVVRHMRTRPYELVAFHSRVFTWQPGDILSTGCPKGARIQPGDRVAARLE
                     GIGFLEATVGRRKAEPFIANGGQTGRSTPFRR"
     gene            complement(995..2644)
                     /locus_tag="VFA54_06110"
     CDS             complement(995..2644)
                     /locus_tag="VFA54_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50414.1"
                     /translation="MVVLAALCLLGLFSTEIADTDFWWHLKTGEYIWQHRSLPVPDPF
                     SFTADLGAPAFEGEARVRYFNLTHEWLGQLLWYAVYRLAGFPGVVLFKALLLTAFCGI
                     AGLLAARRTGSRYWGLAAAGAASSLAVLFSVDRPALLTFFFVAVFVWILERGSPLWLL
                     PVLALVWANCHGGFFLGWVVLGAYAAGATIRRRPGQPEPGLRRLWLITLVSMSVSGLN
                     PSYFHIVEILAAYRRSSLQRGLVEWSRPPLWGPPYAFDVLLYAAIAVLIAARRRVRLS
                     DWLLFSLFAAASLLAFRNILLIAFLAPILIASYFPWQRRLPAAAAPAACLLLAALLGA
                     GIFRGRFFQWRAALWKFPAGAAEFLLANHAGGHLFNTYEYGGYLIWRLWPQQRVFIDG
                     RALNESVYHDYVQALGDSRGGGREEVLARYGVEAIVANGFESTSGVIYPMVLALASPA
                     ETNWRLVYQDPAAMVFFRHPPPGMHGLPKSAIGEHLEAECGLYIEKDPELCLCARTLG
                     FLFLRGGDAQRARRWLSRYLSSPHETDPEAEAAYRGLPVRP"
     gene            2861..3913
                     /locus_tag="VFA54_06115"
     CDS             2861..3913
                     /locus_tag="VFA54_06115"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50415.1"
                     /translation="MNQTKVEELAFRVVGDMGGAFTMALGYIGDRLGLYKAMAGAGPL
                     TSVELAKRTGLNERYVREWANAMVAAEYIDYDPAAQKYIMTDEQAFVLANEDSPMFVG
                     GGLQFTTPSIWNVPKIMEAFRNGGGISYSEIGEEIPEAIERFFRPGYLHSLTNKWLAA
                     VPGLVPRLERGASIADVGCGCGQSTVAMAKAFPKSQVLGIDYHEPSIERARRLAASHG
                     LGNVEFLLAPAEQLPAARKFDLICSFDCIHDMVNPRATLNAIRQALAEDGVYVWSEPN
                     ASEKPHERRNPVAKAFSAISPLHCMTVSLAHDGEGLGTVIGETGARKLAQETGFSKFE
                     KLPIDDPFNQFFALRR"
     gene            3990..4847
                     /locus_tag="VFA54_06120"
     CDS             3990..4847
                     /locus_tag="VFA54_06120"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZS50416.1"
                     /translation="MGNFAGLRVLALESRRAEEIARLITSSGGAPVVAPSMREAPLES
                     NKEALAFASDLRNGRFPVVIFLTGVGARALARVVQSVYPLEEFIDALRKSAVVARGPK
                     PAAVLREWGVPIAVAVPEPNTWRELLRSLDEHSESAPVRGCRVAVQEYGAPNPELLAG
                     LAERGAIVTRVPVYQWTLPENTGPLRDAVAALAREEIDVVLFTTSIQIEHLLQVAGEM
                     GLVEPVRRALRKAVLASIGPTTSEALREHGLAPDLEPSHPKMGFLVKEAAAQSSALLA
                     AKRSRQPAP"
     gene            complement(5001..5076)
                     /locus_tag="VFA54_06125"
     tRNA            complement(5001..5076)
                     /locus_tag="VFA54_06125"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5041..5043),aa:Thr,seq:cgt)
BASE COUNT         1029 a         1569 c         1682 g          905 t
ORIGIN      
        1 gcggattttg gctgaagttg cgtttcgtga agctcagcgc cggaaggggg tgctgcgccc
       61 cgtctggccg ccgttggcta taaacggttc ggctttgcgc cggccgaccg tcgcctcgag
      121 aaatccaatt ccttccagcc gcgccgctac acgatcgccc ggctggatgc gcgcgccttt
      181 ggggcacccg gtcgagagaa tatcgcccgg ctgccaggta aagacgcggc tgtggaacgc
      241 caccagctcg tacgggcgcg tgcgcatgtg ccgcacaacg tcgcgcgaga aaaccgagcc
      301 attatgctcg gtgatcacct cgatggcgtt gacgtcggca atttcgtcgg ctgtggtgat
      361 gatcgggccg aaagaaaaga atgtgtcgaa gcttttggcc cgcgtgaggt agcgcgtatt
      421 gcgccgcagc acgtccagcg cggtcatgtc cagcgtggtt gtgtaaccga ggatcacttc
      481 gggcgcgagc tccggcggaa tgtcccggca ttcctttccg atcacgaccc ccagttcgcc
      541 ctctgcgtcc acttcgctcg aaacctccgc cggcggcaga aggatctccc cgccgggctc
      601 aaacagggcg ctggagggtt tcatgaagct accgggttct tcgggctggg cggcctggag
      661 atcctcggcg tgggacaggt agtttaatcc gatgcaccag atcttgggcg gcgcggggta
      721 gggcagacgc aggcgtattt cggccggcgg caaagccgcg ccgtggagat cttcgacgct
      781 ggcattcttg agatcctcga tccggcccgc cgtgatgagg gcgagcagat cgtcggggaa
      841 gtcgccgccc cgccgggcgt tgatttcggc caccggcagc acgcggcgct cgccgccttt
      901 cgcgcgcacg accacgccgg cgtatatctt cgagtgatgt tcaaatgtgc aaagcttcat
      961 gagtttggcg aactgctttc agtttcgctc cgggctatgg ccggacgggc aagccgcgat
     1021 aggctgcctc ggcctccgga tctgtttcgt gggggctcga cagataccgc gagagccagc
     1081 gccgcgcccg ttgcgcatct ccgccccgta gaaacaaaaa acccagtgtc cgcgcacaca
     1141 ggcatagctc gggatccttc tcgatgtaca gcccgcattc agcttccaga tgctccccga
     1201 tcgcggactt aggcaatccg tgcattcccg gcggcggatg gcgaaaaaaa accatggccg
     1261 ccggatcctg atagaccagg cgccagttgg tttcggcggg gctcgccaac gccaagacca
     1321 taggataaat cacgccgctg gtggactcga agccgttggc tacgatcgct tccactccgt
     1381 aacgggccag cacttcctct cgcccgccgc cgcgggaatc gcccagcgcc tgtacatagt
     1441 cgtgatatac agattcgttc agcgcccgcc cgtcgatgaa cacgcgctgc tggggccaaa
     1501 gccgccagat cagatagccc ccgtattcat aggtattgaa aagatgcccg ccggcatggt
     1561 tggcgagcag gaactcggcc gcgccggcgg gaaatttcca caaagcggcc ctccactgaa
     1621 aaaatcggcc gcggaagatg ccggctccca gcagagcggc cagcagcagg cacgccgccg
     1681 gcgccgcagc agccggcaag cgccgctgcc aggggaaata agaggcaatc aggatgggcg
     1741 ccaggaaggc gatgaggagg atgtttcgga acgccagcag tgaggcggca gcgaacagcg
     1801 agaacaacag ccagtcgctc aggcgcaccc gccgccgcgc cgcaatcagc accgcgatag
     1861 cagcatacag cagaacgtcg aacgcgtacg gcgggcccca gagaggcggc cggctccatt
     1921 ccaccaagcc gcgttgcagc gagctgcgcc ggtacgcggc gagaatctcg acgatgtgga
     1981 aatagctggg atttaggccg gagacggaca tggaaaccag ggtaatgagc caaaggcggc
     2041 gcaggccggg ctcgggctgc cccggccggc ggcggatcgt agctccggcg gcgtacgcgc
     2101 cgagcacgac ccaaccgagg aaaaaccctc catggcaatt cgcccacacc agcgccagga
     2161 ccggcagcag ccacagtggc gagccgcgct ccagaatcca gacaaagacg gcaacaaaga
     2221 aaaacgtcaa tagagccgga cggtccacgc tgaaaagcac ggcgagggaa gacgcggcgc
     2281 cggcggcggc cagtccccaa tagcggctgc ccgtccggcg cgccgccaga aggcccgcaa
     2341 tgccgcaaaa tgcagtgagc agcagcgctt tgaagagaac cacgcccgga aaacccgcga
     2401 gacggtaaac ggcgtaccac aagagctggc ccagccactc gtgcgtgagg ttgaaatagc
     2461 gcactcgcgc ctctccctcg aacgcgggcg cgccgagatc ggcggtgaag gagaaaggat
     2521 cgggaacggg cagcgaacgg tgctgccaaa tgtattcgcc ggttttcagg tgccaccaaa
     2581 agtccgtgtc ggcgatttcg gtggaaaaca agcccaaaag gcagagggcg gccaggacca
     2641 ccatcgccgt ccccagccac cggtccggga gtcttgaagg ccgcggcggc tgcggagcag
     2701 actcggacgt cgcgagaatg ggctttgtgg gtccggcctt tccagggacg gaggctcttg
     2761 aagctttccg tgacagaagc gatagcttat cacaatgccc gcgagcagcc ttgtgcggta
     2821 ccatttagag caagttcggc tttgaaggag gatcacaggt atgaaccaga caaaagtgga
     2881 ggagcttgcg ttccgcgttg tgggggacat gggcggagca ttcacaatgg ccttgggtta
     2941 catcggcgac cgtctcggtc tttacaaagc aatggccggg gctggcccgc tgaccagcgt
     3001 cgaactggca aagagaaccg gcctcaacga gcgctatgtc cgcgagtggg ccaatgctat
     3061 ggtggcggcc gaatacattg actacgaccc agccgcacaa aaatatatca tgacggacga
     3121 gcaggcgttc gtgctggcca acgaagacag cccaatgttc gtcggcggcg ggctgcaatt
     3181 caccacgcct tcgatctgga atgtgcccaa aatcatggaa gcctttcgaa atggcggcgg
     3241 catctcttat tctgaaatcg gcgaggaaat tcccgaagcg atcgagcgat tcttccggcc
     3301 gggctatctc cattccttga ctaataaatg gctggccgca gtgcccggtc tggtgccgcg
     3361 cctggagcgg ggcgcatcca tcgccgatgt gggctgcggc tgcggccaat ccactgtagc
     3421 catggccaag gctttcccga aatcgcaggt gctgggcatc gattatcacg aaccgagcat
     3481 cgagcgggcg cgaaggcttg cggcttccca cggcctcggc aatgtggagt tcttgctggc
     3541 tccggcggag cagcttccgg cggcccgcaa gttcgacctg atttgcagtt tcgattgcat
     3601 tcacgacatg gtcaacccgc gggcaacgct taacgcaatt cgccaggcgc tcgccgaaga
     3661 cggagtttac gtctggtcgg agccaaacgc ctcggagaag ccgcacgagc gccgcaatcc
     3721 ggtggcgaag gccttttcgg cgatcagccc gctgcattgc atgaccgtgt cgctggcgca
     3781 cgacggcgag gggcttggca cggtgatcgg cgaaacgggc gcgaggaagc tggcccagga
     3841 aacaggcttt tcgaaattcg aaaagctgcc gatcgatgat ccgtttaacc agtttttcgc
     3901 tctgcggcgc tgaaagcggc ggcgctgttg ggagcagatg agttcctgaa gcggggcttt
     3961 tacacggctg ccgcgggctt gactttggaa tgggtaactt tgccggtttg cgtgttctcg
     4021 cgctcgagag ccgccgcgcg gaagagattg ccaggctgat taccagcagc ggcggcgctc
     4081 ccgtcgttgc tccctcgatg cgggaagcgc cgctcgaatc caacaaagaa gcgctggcat
     4141 tcgcctccga tttgagaaac gggcgcttcc ccgttgtaat cttcctcacc ggcgtcggcg
     4201 cccgggcgct ggctcgcgtg gtccagtctg tctacccgct ggaggagttc attgacgctc
     4261 tcaggaagtc ggcagtggta gcccgcggcc ccaagccggc ggcggtgctg cgggaatggg
     4321 gtgtgccgat tgcagtggct gtccctgagc ccaacacatg gcgcgagctg ctgcggtcgc
     4381 tcgatgaaca ctccgagtcg gcgcccgtcc ggggctgccg ggtcgccgtg caggaatatg
     4441 gcgctccgaa tccggagctg ctggcggggc tggccgagcg cggcgctatt gtgacgcggg
     4501 tcccggttta ccaatggact ctacctgaga ataccgggcc gttgcgggat gccgttgcgg
     4561 cgcttgctcg agaggaaatc gatgttgtcc tgtttacgac ttccatccaa atcgagcacc
     4621 tgctccaggt agccggggaa atgggactcg tggaaccggt gcggcgcgct ttgcgcaaag
     4681 cggtcctcgc ttcgatcggg cccaccacat cggaagcgct tcgggagcac gggctcgcac
     4741 ccgatctgga gccgagccat ccgaaaatgg gttttttggt caaggaagcc gccgcgcaaa
     4801 gcagcgcatt gctggcggca aagcgaagcc ggcagccagc tccttagtta gcaggctcga
     4861 agaaatttcc gcctgatcgg agaaacagcg gctgccaggc tcgaacggct gcggcttgcg
     4921 tcttgctccc gggaatctcg ggcctttggt gtaatcccag taagtctttc gaagaatgga
     4981 gccgcgggcc ggttcgaaaa tggagccacg ggtcggactt gaaccgacga cctgcggttt
     5041 acgaaaccgc tgctctaccg gctgagctac cgtggctgtt tcgctgcgtg tttttagttt
     5101 atcactgaag acgtgcctgg taagacgctc cctattcttc cgcggccgca aggtgcacgg
     5161 cgcagcttaa acgcatggcc ggcaa
//
LOCUS       DASREK010000123        15072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_397165, whole genome shotgun sequence.
ACCESSION   DASREK010000123 DASREK010000000
VERSION     DASREK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397165"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1388..2863
                     /locus_tag="VFA54_06130"
     CDS             1388..2863
                     /locus_tag="VFA54_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50417.1"
                     /translation="MGIDPAAAGIVFEGGFATSSGSWEVKAPAVRFAGSRRILPRPHG
                     GLRSSVVSAVNVWPAERGAAAPNLSHCGIIAPRVPVDSRMVQAPRELPVRVSVLPRQS
                     SPRVDAADFLGKAADPALPDRPIDPPFRTRPGADLAFGPPKVNASAAAPPAAQWLPLA
                     AVERVEIGPEPLPMLSAAPVETVTPPRSMPAAPLRSAGETAESASRPPMPSAGAPPDA
                     LREAVARAQQARSDRGSELARRVQMAWEEALWPALNTKAGRMGLAVCAGFLALLFGIH
                     AARPAIGSGLNSLLKPLNDRSYFLLEEGFDKGFNNWTNPGLMTPSQGGLTELQRGLTL
                     YRPSLHRADYEFTFSGLVRRGALGWVVRAADSRNYYAFKLAWQGRGKERRSVLLRYPV
                     ANGASADKPQAVPLPFDLIEDKLYRVTVDAAGGRITTMINDRGVDSCADANLKLGGVG
                     FFADVGDEEMIQSLSVSGNDDGMGRLIYWFKGFGAFLADKL"
     gene            3048..4769
                     /locus_tag="VFA54_06135"
     CDS             3048..4769
                     /locus_tag="VFA54_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50418.1"
                     /translation="MRIFGDMTANGEKNKEAGPQRSGDGTRARAAIAGAAEERRSSSN
                     GHDPNEFFATAVALHMQGKPEEALIYLEKARKSGENLAEIYSAMAQIYLERKQHSEAA
                     KAYRDLLAVDPDNVAAEFNLALCYEALGSYPEACKAFRAVIHNSPELVDAHLGLAAAC
                     TKMGDFAAAQAAYEEYLKTNPNSFAARFGLGVSLHLAKQYESAIAAYKKALEMEPQSE
                     EALANLASVYSETQDFKHALACYDKLVKAKPDSWTAQEGLAYASFRMELFEEAAEHYE
                     AVVKLNPQSFESWYNLGVTRQKLGAAKEAAAAYREAARLKPDAVEPHINLAIALEELG
                     EEADAMTEYQHALRLDPKAAAPLYNLALIYEKKNKEIEAADCYQRLLQEKPDFLDARF
                     RLALLFHKRGDWARAAAQYEQCLKEKPEWPEAWLNIGIVYLKSSRLPEAENAFTQCLK
                     LTPEDPEAMLGLAAVHLQQENWEQARKLYQRLIELKPNSPEILFNAALLEQKSKNLQE
                     AIKLYSQAVSLKTEFAEAYLNLAIAQQASGHEADASKAFEKAILLKPELARGYFTLAH
                     PGSTAVQ"
     gene            complement(5006..6934)
                     /locus_tag="VFA54_06140"
     CDS             complement(5006..6934)
                     /locus_tag="VFA54_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50419.1"
                     /translation="MQLRRFARFLFFLTLAFLSRCAAALASEADALAISANIQARHMP
                     HGTILDPIFAAPDSDQIIRYTRCGDSAIWTGHYLAAEAFRYKVTGSADALANAKKALA
                     GIKSLADVTGTNLLARCLVPVDSPYAPSITQEEQHNGVYTNSSAKYYWVGGTSRDQYS
                     GVFFGLGVAFDMIDDAGVRAAIRDLTTRLLDFLRGRNWMVVMPDGSISTTFIARADQQ
                     LSFLQVGRHVNPDHYSLTLDKTILSPEAPLPIAIDVTTDSSYFKFNLDSINLYNLIRL
                     DGSIFNPLYVQAYNVLRRHTRDQENAFFNMIDRALNGPDAVRDQATATMLDQWLQRPR
                     RDFFVDLRGQLSSCGSPDQACSPIPVPLRVPTDFLWQRSPFQLVGGGSGVIENAGIDY
                     ILPYWMARYYGIENADSVVSAASGGTSVSAESIASFYGSGLASGVDSAKSLPLPLTLG
                     GVRVQVQDSAGVSRMAPLFYVSPTQINLEIPAGTAAGQATVTVLDANNTALVSTTADV
                     QAVAPGLFTADGSGKGVAAALAIQVGVNNLQNVTPIFDCAGGVCASVPIKLGVDRPVY
                     LSLYGTGIRNRSSLANVTVVISATNAPVLYAGPQGSYAGLDQVNVAIPLSLRGAGETD
                     LLLTVDGKAANSVRVNIQ"
     gene            complement(7052..7393)
                     /locus_tag="VFA54_06145"
     CDS             complement(7052..7393)
                     /locus_tag="VFA54_06145"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HZS50420.1"
                     /translation="MPAETPAKLLRIHFSERDRYGGKPLYEAIVEKCRELKIAGATVF
                     RGFEGYGEKAELHKRHLLGHDLPIVVTIVDSAQNIERLLPAIEEMMDTGMIAISDVSV
                     RRIQKAAEAER"
     gene            complement(7371..8105)
                     /locus_tag="VFA54_06150"
     CDS             complement(7371..8105)
                     /locus_tag="VFA54_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HZS50421.1"
                     /translation="MLQKGPAKKVTIFVNEDAQSHATPLYEAILTFLLHKGVAGATAT
                     RAMAGFGAHQVMHTTKIEVLTEHLPIRIEFIETPEKVNELLPILYDMVTDGLIEVQDT
                     QVLKAAKKERRAAPKVPHQEKRGKATLMRIYLGESDKWHGEPLYDAIVNRLRMIDVAG
                     ATVYRGVMGYGAKGHTHKQSFWHLSRDLPVMISVIDEPEKVARASDAIEEMIGDGLIV
                     LSDVDMIRLVHNQPAVEAADAGGNAR"
     gene            complement(8121..8492)
                     /gene="crcB"
                     /locus_tag="VFA54_06155"
     CDS             complement(8121..8492)
                     /gene="crcB"
                     /locus_tag="VFA54_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HZS50422.1"
                     /translation="MERYLMVLVGGGTGALARYLAGSAIMSRIGGRFPLGTLLINVSG
                     SFFIGLLMTLFTERFQPHPNWRLLLVVGFLGGYTTFSSFEWETYSAVREGGRWVGFVN
                     VAASVILGYAAVWLGSLLARR"
     gene            complement(9046..10542)
                     /locus_tag="VFA54_06160"
     CDS             complement(9046..10542)
                     /locus_tag="VFA54_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS50423.1"
                     /translation="MSQLLQTKSIDQLVAQAEAPERRLRKSLGPWSLTALGVGAVIGT
                     GIFVLTGTAAAGETVAIPSLLKAPVLDLLLHGRRAVSTIGRPGAGPGIALSYLLTAIA
                     CGFAALCYAELASMIPIAGSAYTYAYATLGEIFAWIIGWDLILEYAIGNTAVAVGFSG
                     YFNDLLQNLLGVCLPLRWSTPPIAGGAYTGSWFNVPATLVMFALTVLLVSGVREGARA
                     NAVMVGVKVTAILVFVFGAARAVRTEHWHPFLPNGFQGVLTGAAIVFFSYIGFDSIST
                     AAEECKRPQRDLPLGIIATLAICAVLYVAVALVLTGIADWRTLNNAAPVANALKPLGM
                     EQVRRWVGIGAMAGMLSVLLVFQYGQARIWFAMSRDGLLPPVFSRIHPRFQTPHVSTW
                     IAGVAAGVPAGIWDIGTFADLTNIGTLFAFVVVSAGVILLRRRQPERRRAFRVPWVPL
                     LPLVSAGSCLVLMASLPLETWIRFFVWLTIGLGIYLLYGRRHSQFAGK"
     gene            complement(10709..13102)
                     /locus_tag="VFA54_06165"
     CDS             complement(10709..13102)
                     /locus_tag="VFA54_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZS50424.1"
                     /translation="MDFLQQLNPKQREAVEHTEGPLLILAGAGSGKTRVITYRIAHLI
                     CHRGVWPGNILAVTFTNKAAGEMRDRVLALLAENGGAELAGVSPAVSTFHSFCVRLLR
                     RDGAPLAALRAGFTTRFTIYDEADQLAAVKAVYKQLGLDEKFLAYRGALSRISHAKNH
                     GWTPEEYFRRSTNPQAEKLAVVYEQYEAALRRTGALDFDDLLLEAVRLLRHDEGARLR
                     AAERYHYIMVDEYQDTNRPQYELVRLLCEPRRNICVVGDEDQSIYAWRGADIRNILDF
                     ERDFPGAVLIRLEQNYRSTKNILDAAGHVVAHNKERKGKKLWTEAGAGSPLWLYTAAD
                     AENEALFIADTIEKYLRENPGDRVAVLFRTNFQSRQIEEALRRYGRRYVLVGGLSFYQ
                     RAEIKDLLAYLKAAVSPQDSFSLLRIINTPARGIGKSTIEQIEKYALEREISLWSAMQ
                     ELLRTGAFPSRAQAAVTAFCGLMDELKSRLQSERLNDTLVWLKQRTGYQRMLEEEDTV
                     ESQSRMENIDELINAAADAAERGEGVNEFLDHAALVADTDQLDEGAQVTLLTLHSAKG
                     LEFSLVIMAGMEEILFPHIRASETESGIEEERRLCYVGMTRARKQLILTRARYRRRFG
                     GGELEPSLPSRFLSEIPVSLIQDLSPAPEPGVIPGLDLYAEQEIVRQMARRSAYTGKT
                     YNSIENIAEFFAARGIRAPQPVPQAAPSAGGAAATGPASVAAGAHPSPPAGPAGAPAR
                     RSKASHFRAGTRVHHPKYGTGTVLRREGEGDDAKITVHFLHYGIKKLIEKYATLRQE"
     gene            complement(13439..14410)
                     /locus_tag="VFA54_06170"
     CDS             complement(13439..14410)
                     /locus_tag="VFA54_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50425.1"
                     /translation="MKMEAIAVKDSAGRLLFHPIFRPSGKKLLAKGHQLSEEDVRLLD
                     AEGLERIWVAALEQGEVPEDYAASMMASEIGCGSLEIKLAAGGRANLVATENSCLLVD
                     DQLLRQVNSAGCLAVATSPNFSYAVAGQRVATIKTAPFAIPEREMEATRAMLRECGPV
                     LQARPIRHPVVAVLYSDLLQGDRARQQFESIMRIRLQRLGTGSTFVLTAIEEEPAVAR
                     ALDHLLRSHPTIVLIASTTAPAGPCDVVGRAMERIGCKLERFLAPVEPGNLLLLAYAG
                     DVPVVSAPGCFRSPKPNVVDLLLPPLLARYRVAAHEVASLGHGGLLQ"
BASE COUNT         2953 a         4780 c         4572 g         2767 t
ORIGIN      
        1 ggcgtcctcg ctgccggccg aagcgttccg catcgaaccc agcatgggat tcgggcggca
       61 cgtggagagt ttttccccgc tcggcccccc tctctcgatc tggcgcagcc gaccgggaga
      121 ctcaccggca gcgacgacgc caggtattcg atcgttttcc acccgcggtc ttcggctgcc
      181 tcacgagcag aggtggatca atccggcgga actggcgact ccgaaagcgg aaacttcgcg
      241 ctccttgcgg caggcctgga gtttggacgg ccccttgttc cggggcggcg tgttgctgcc
      301 ggtcgggcgg tatgcggccc atgctcccat ccacaacggc gggctggcaa ccgtgccggc
      361 cgcgcaggcc tttgcgctgg tccggattgc tagcgccgga gcggaactcc aggcgccccc
      421 gcgggccgac ccggagcttg tgaacccaag cccggctatc aagcgcccaa gcccggcgat
      481 gaccggcggg ttttccttta cagctttgag gaaagaaaca gcggctttcc gcactctgat
      541 ggcggccctc cgcgaatcga gcccaaaact ccgggtggaa gaggttttgc ccatcggtcc
      601 gaagccggcg agggcgcccc tgcaatcgcc ggcgacgccc tgcccgcgcc aactgggcca
      661 cttgggcgag cacgccgcgc ttcgctggag acaaagagtc ccgccgccga gatggcagac
      721 gccggccccg gaactgcttg ttccggccct ggccgccgaa ctaacagcgc aagattccag
      781 ggactgccgc aagccggttg tccctgcggg cgcagtgcag gcggcggcca ttctcatacg
      841 ccttccaggg accggtctga ggccgctccc cgcaacgggg cgcgcgccgg cggtcccgtt
      901 cactccattg ggcgcagaac cccgagcgcc ggccgcggcg ccgcttctct ttcccccgca
      961 agtttacggt cgccggccga atttccatgc ggcatgctcg cgaaacttcg cgcgaatttc
     1021 gcgtttcggt tccggggaaa tgctctcggg cagccttgct ctgtttccga tgcttgccgg
     1081 gagagccgca tcggcgatcg ttcccccagc ctctccgaaa agattacggt caggcctgaa
     1141 accgccgatg cgctattcct acgtgcaaac ggccggcgca aacgggatcc ttacagccca
     1201 aaacatcccg gcgcggcagc cgcaagcctg gatctctacg gagccggcgg cggttgcttc
     1261 cgcgaccccg cagcgaatcg caacttttcc tgcaccgcat ttcccaaggg cgcaggtgcg
     1321 gatgcaatcg tgtccgggtg cgcggcccag agtagccgcg gcgaggcctg tactcgagca
     1381 gccggccatg ggaatcgacc cggccgcggc cggtatcgtt ttcgaaggcg gtttcgctac
     1441 ttcgagcggt tcctgggagg tcaaggcccc cgcagtccgg tttgcggggt cccgccggat
     1501 cctgcctagg ccgcacggcg gcctgcgctc atccgtggta tccgccgtga atgtgtggcc
     1561 ggcagagcgc ggcgcggcgg cgccgaatct ttcgcactgc gggatcatcg cgccgagggt
     1621 tcccgtggac agccggatgg tccaggctcc gcgcgagctg cccgtgcgcg tctccgtttt
     1681 gccgcgccag tcttctccga gagtggatgc agcggatttc ttggggaagg ccgcggatcc
     1741 ggctctcccg gatcgcccca ttgatccgcc gtttagaaca aggccgggcg cggacctcgc
     1801 cttcgggccg cccaaggtta acgcctctgc tgcggctccg cctgcggcgc agtggctgcc
     1861 gctcgcagcg gtcgagcgtg tggagatcgg gccggagccc ctgccgatgc tttctgctgc
     1921 gccggtcgag acggttaccc cgccgcggag tatgccggcc gccccgctgc gatccgctgg
     1981 agagaccgcg gagtcagctt ctcgcccccc gatgccgagc gccggagctc cgcccgacgc
     2041 cctgcgggag gcggtcgcac gggctcagca ggctcgctcg gatcgaggca gcgagctcgc
     2101 ccggcgagtc caaatggcgt gggaagaggc gctctggccg gcgctgaaca ccaaagcagg
     2161 ccgcatgggc ctggccgtct gcgccggatt tctcgccttg ctgttcggta tacacgccgc
     2221 ccggccagcg attgggtcag gcctgaatag tctgctgaaa ccgctgaacg accgttccta
     2281 cttcttgctc gaagaagggt tcgacaaggg attcaacaat tggaccaacc ccgggctcat
     2341 gacgccctcg caaggaggcc tgaccgaatt gcagcggggt ctcactctct accggccctc
     2401 gctccaccgg gccgactacg aatttacttt ttccggtctg gtccggcgcg gcgctctcgg
     2461 ctgggtcgtc cgcgccgccg actcccgcaa ttactacgct ttcaagctgg cctggcaggg
     2521 cagagggaaa gaacggcgca gcgtgctgct ccggtatccg gtggcgaacg gcgcctcggc
     2581 cgacaagccg caggcggtgc cgctgccctt cgaccttatc gaggacaagc tgtaccgcgt
     2641 cacggttgac gcggccgggg gccgcatcac cacgatgatc aatgaccgcg gcgttgattc
     2701 ctgcgcagat gccaatctta agctgggagg cgtgggattc ttcgccgacg taggcgatga
     2761 agaaatgatc cagtcgcttt cggtaagcgg taacgacgac ggcatgggaa ggctgattta
     2821 ctggttcaaa ggcttcggag cgtttctggc ggataagctt tgaaacctat gtctcagaaa
     2881 ttcattggag atttccgagc agcgcccccg gggccgggcg ggagctttga agcgtcgggc
     2941 ggcgggcatg gacgctgcgc cggtcccgcg cgaagaattt tgcagcgaat gtctgatcca
     3001 gcgcctcgat cttcggccag gcgcgacttg ttagcaggga gaatctcatg agaattttcg
     3061 gcgatatgac agcgaacggc gaaaaaaaca aggaggcggg tccccagcgc tcgggcgacg
     3121 ggactcgggc gagggcggca atcgccggcg ccgccgagga acgccgctcc agctcgaatg
     3181 gtcacgaccc caacgaattt ttcgccaccg ccgtggctct gcacatgcag ggcaagccgg
     3241 aagaagctct catttacctt gaaaaagcgc gcaagagtgg tgagaacctg gcggagatct
     3301 actcggcgat ggctcagatt tacctcgagc gcaagcagca ctcagaggcg gccaaagcct
     3361 atcgcgacct gctggccgta gatcccgaca acgtcgccgc cgagttcaac ctcgcgctct
     3421 gctacgaggc tttgggatcc taccccgagg cttgcaaagc gttccgggcg gtcatccaca
     3481 acagtccgga attggtggat gcgcaccttg gcttggccgc cgcctgcacc aaaatgggcg
     3541 atttcgccgc ggcccaagcc gcctatgaag aatatctaaa aaccaatcca aactcgttcg
     3601 ccgcccgctt cggcctgggc gtgagcttgc atctggccaa gcagtatgaa agcgccatcg
     3661 cggcgtacaa gaaggcgctc gaaatggagc ctcagtcgga agaggcgctg gccaatctgg
     3721 cttccgttta tagcgagacg caggatttca agcacgcgct cgcctgttac gacaagcttg
     3781 tcaaggccaa gccggattca tggaccgcgc aggagggcct cgcctatgcc agcttccgga
     3841 tggaactctt cgaggaagct gcggaacatt atgaggccgt cgtgaagctg aacccacagt
     3901 cttttgagag ctggtacaac ctgggcgtca cgcgccagaa actgggagca gccaaggaag
     3961 cggccgccgc ctaccgcgaa gccgcccggc tcaagcccga cgccgtcgaa ccgcacatca
     4021 acctggccat tgcgctcgaa gaactgggag aggaagcgga cgccatgacc gaatatcagc
     4081 acgcgctccg cctggatccg aaagcggcag ccccgctcta caatctggcg ctgatctacg
     4141 aaaagaaaaa caaggagatc gaagccgccg actgctacca gcgcctgctc caggaaaagc
     4201 cggactttct cgacgcgcgt ttccgcctgg ctctgctgtt tcataagcgc ggggactggg
     4261 caagagcggc cgcacagtat gagcaatgcc tgaaagagaa accggagtgg ccggaggcgt
     4321 ggctgaatat cggcatcgtt tacctcaagt caagccgatt gccagaggcg gaaaacgcct
     4381 tcactcagtg cctgaagctc acgccggagg acccggaggc gatgctcggg ctcgccgcag
     4441 tccatttgca gcaggagaac tgggaacagg cgcgcaagct ctaccagcgg ctgatcgaac
     4501 tgaagccgaa tagccctgaa attctgttca atgcggcgct gcttgagcag aagtccaaga
     4561 atctccagga ggccatcaag ttgtacagcc aggcggtttc cctgaagacc gagtttgccg
     4621 aggcctatct caatctggcg attgcgcagc aggcctctgg acacgaagcc gacgccagca
     4681 aggccttcga aaaggcgatc ttgttaaagc ccgaattggc ccgcggctat ttcacgctgg
     4741 cgcaccccgg ctctaccgcc gtgcagtaga tggcgggcgc aggccggcgg tggaaacagc
     4801 ggcgccgcaa accgcaccag ggcgccgcac gagtgcggcc cagcaacgag gcggcgcgac
     4861 ggccctaccc aggcggcgct aaaggcgtga tgttagcaac cgtgcttgag caacgggccg
     4921 cggctcgcaa ctttcatcgc cccgcgcgac acgctgcttg ttccggcggc cctcgaatca
     4981 gcggcgggcg ggagcgatcg cggttttatt gaatatttac ccgcacgctg ttggccgctt
     5041 tcccatcgac tgtcagaagc aggtcggtct cgcctgctcc ccgcaagctg agcggtattg
     5101 ccacgttcac ctgatccagg ccggcgtagg agccttgcgg ccccgcatac aggaccggcg
     5161 cgttggtggc gctgattacc acggtaacgt tggcgagcga agacctgttc cgaatccccg
     5221 ttccatacag gctgaggtat acgggcctat ccactcccag tttgatggga accgatgcgc
     5281 acacccctcc ggcgcaatcg aaaatcggag tgacgttttg caggttattg acacccacct
     5341 gtatcgccaa ggccgcagcg acccccttcc cgctcccgtc tgctgtgaat agcccggggg
     5401 cgaccgcttg cacgtcggcc gtggtggaga ccagagcggt gttatttgcg tccaagacgg
     5461 taactgtggc ctgtccggca gcggttccgg cgggaatttc aagattgatc tgtgtggggg
     5521 aaacatagaa caagggcgcc atacgggaaa cgccggcgct gtcctgaacc tgaacccgca
     5581 cgccgcccag cgtcagcggc aagggcaggg attttgcaga gtctacgccg ctcgccagac
     5641 cggagccgta aaaagaagcg atcgattccg ccgaaaccga ggttcctccc gaggcggccg
     5701 atacgaccga atccgcgttc tcgattccgt aataccgcgc catccaatac ggcaggatgt
     5761 agtcgatacc cgcgttctcg atcacgcccg atcctccgcc aaccagttga aacgggctcc
     5821 gctgccacag aaaatccgtc ggcacgcgca agggaacggg aatcggcgag caagcctgat
     5881 ccgggctgcc gcaggaagac agttggcccc gcaggtcgac aaaaaaatcc ctccgcgggc
     5941 gctggagcca ttggtcgagc atggttgccg tagcctgatc gcgcaccgca tccggcccgt
     6001 tgagggcgcg gtcaatcatg ttaaaaaatg cattctcctg gtcccttgtg tgccgccgca
     6061 gcacattata agcctggaca tagagaggat tgaaaatgct tccgtccagg cgaatcaggt
     6121 tgtacagatt gatgctgtca agattgaatt tgaagtagga gctgtccgtc gtaacgtcga
     6181 tggcgatggg cagcggcgcc tcgggagaca gaatggtctt gtccagcgtc agagagtagt
     6241 gatcgggatt gacgtgacgt ccgacttgca gaaagctcaa ctgctggtcc gcgcgggcga
     6301 taaatgtcgt ggagatggag ccgtccggca tgacgaccat ccagttgcgt ccccgcagaa
     6361 aatccagcag ccgggtggtc aaatcgcgga tcgccgcgcg aacgccggcg tcatcaatca
     6421 tatcgaaagc cacgcccagc ccgaagaaaa ctccagagta ttgatcgcgc gacgtcccgc
     6481 ccacccagta atatttggcc gaactgttcg tataaacgcc gttgtgctgc tcttcctgcg
     6541 tgatcgacgg ggcgtaaggg gaatccaccg gaaccaggca gcgcgccaga agattggtcc
     6601 ccgtcacgtc ggccagcgac ttgatgcctg cgagcgcttt tttggcgttg gccagcgcgt
     6661 cagcggagcc ggtcacctta tagcgaaagg cttccgccgc cagataatgg cccgtccaga
     6721 tcgccgagtc cccgcaccgc gtgtatcgta tgatctgatc gctgtccgga gcggcgaaga
     6781 tcggatccag gatcgtcccg tgaggcatgt ggcgcgcctg gatgttggcc gagatcgcaa
     6841 gggcatccgc ctccgacgcc agcgcggcgg cgcatctgga aagaaaagcg agtgtgagaa
     6901 aaaagagaaa acgcgcgaat cggcgcaact gcaatcggcc ctcaccgtgt ctgagcggac
     6961 agactggcga tcgtagcaga cccgctcgca gcttgtcggt tcgatacagc acgttccggg
     7021 tctttctgcg cgggcccaag cagccgcgcc ttcagcgctc cgcttccgca gccttctgaa
     7081 ttctgcgtac gctcacgtcc gatatggcga tcattccggt gtccatcatc tcttcgatgg
     7141 ccggaagcaa tctttcgata ttctgggccg aatcgacgat ggtcaccacg atcggcagat
     7201 cgtgcccgag gaggtggcgt ttgtggagct ccgctttctc cccgtagccc tcaaagccgc
     7261 ggaatacggt ggctccggcg atcttcagtt cccggcattt ctcgacgatc gcttcgtaga
     7321 gcggcttgcc gccgtagcgg tcccgctcgg aaaaatggat gcgcaggagc ttagcgggcg
     7381 tttccgccgg catcggccgc ctccaccgcc ggctggttgt gtaccaatcg tatcatatcg
     7441 acatccgaca gaacgatcag gccgtccccg atcatctcct cgatcgcgtc gctggcccga
     7501 gccacctttt ccggctcatc gataacggag atcatgaccg gcagatcgcg ggacaggtgc
     7561 cagaagctct gcttatgggt atggcccttc gcaccataac ccataacccc ccggtatacg
     7621 gtggcgccgg ccacgtctat catgcggagg cggtttacga tggcgtcgta gagcggttct
     7681 ccatgccact tgtccgattc gcccagataa atgcgcatca gagttgcttt cccgcgcttt
     7741 tcttgatgcg gcaccttcgg cgcggcgcgg cgctccttct tcgccgcctt gagcacctgc
     7801 gtgtcctgca cctcgatcag gccgtcggtg accatatcgt agaggatggg gaggagttca
     7861 tttactttct ccggggtttc aatgaactcg atgcgaatgg gcagatgctc tgtcagcact
     7921 tcgatcttgg tggtgtgcat cacctgatgg gcgccgaatc cagccattgc ccgcgtggca
     7981 gtagctccgg ccactccttt gtgcagcaga aatgtaagga tcgcctcgta gagcggtgta
     8041 gcgtgggatt gcgcgtcttc gtttacgaag atcgtaactt ttttggcagg ccctttttgc
     8101 agcattcctc ctcctctgcg ctagcggcgg gccagaagcg atcccagcca tacggccgca
     8161 tagcccagta taacgctcgc ggcgacgttg acgaagccga cccagcggcc gccctcccgg
     8221 acagccgaat atgtctccca ttcgaaactg gaaaaggtgg tgtagccccc gagaaagccg
     8281 accaccaata acagtcgcca gttgggatgc ggctggaacc gctcggtaaa caaggtcatc
     8341 agcagcccaa tgaagaacga gccgctcacg ttgataagaa gggtaccgag aggaaaacgc
     8401 ccgccaatcc ggctcataat ggccgatcca gccagatatc gcgccagcgc cccggtaccg
     8461 ccgccaacca aaaccataag atatcgttcc aaacgctaga cctcccactc cggtcgagtt
     8521 gagcgggagg cccgacgcac cgcggatccc agaggctcca acgcggccga acccctctcg
     8581 aaaccgatca agcctttggc gatccaagtt tgggcgcggt ccggtaaagt tgtggttgtc
     8641 gcatggggaa tcccgtcgtt cgccttgctg ctcctggcag aaaacgccgc ttgtaagttg
     8701 tgcatgttcg ttccggtccc ttttgcatca taagccaatt gcgcggttgc gccgcaattc
     8761 cgaagcggcg ggcggcaatg gcgctgccgg ccctgggttt accgaaacgg cgctgcataa
     8821 gcggcggcaa agccatcgtt cagggaatca caggcaggat ccagctcggc gagaagctcc
     8881 tgcaagcgac ctttttaccg tagtcattgg cctcgatgga ggagcggcga ttgctcgaac
     8941 tccacggcgt gaaccgccct ttcaagctaa tcgataaggc ttgccagcgt caattttgca
     9001 aataaaaatc gatgcactcc ggcccgctcc tgtccggggc gcaatctact ttcccgcaaa
     9061 ctggctgtgc ctgcggccgt aaagcagata aattccaaga ccaatcgtca gccaaacaaa
     9121 aaagcgaatc caggtctcca gcggcaaact ggccataagc accaggcagg accctgctga
     9181 aaccaggggc agcagcggga cccacgggac gcgaaaagcc cggcgccgct ccggctgtcg
     9241 ccgccgcagt aaaatgacgc cggcggaaac aacgacgaac gcaaacaggg tgccgatgtt
     9301 cgtcaagtcg gcgaacgtgc cgatgtccca gattccggcc ggaactcccg ccgcgacgcc
     9361 ggcgatccag gtgctcacat ggggcgtctg aaatcgcggg tgaatgcgcg agaatacggg
     9421 cggcagcagg ccgtcccgcg acatggcaaa ccagattctc gcctgcccgt actgaaacac
     9481 gagcagcacg gagagcattc ccgccatcgc gccgatgccc acccatcgcc gcacctgctc
     9541 catgccaagc ggcttgaggg cgttcgccac cggcgcggcg ttgttcagag ttctccagtc
     9601 cgcaatgccg gtcaggacca gagcgacggc cacgtacagg accgcgcaga ttgccagagt
     9661 agctataata cctagtggca gatcccgctg cggccgtttg cattcctcgg cggcagtgga
     9721 gattgagtca aagccgatat agctgaaaaa aacgatcgcc gcgccggtca gcactccctg
     9781 gaagccattg ggcagaaaag gatgccaatg ctcggtgcgg acggctcgcg cggccccgaa
     9841 gacaaacacc agaatcgcgg tgacctttac gccgaccatg actgcgttgg cccgcgcccc
     9901 ttcccggacg cctgacacga gcagcacggt cagcgcaaac atcacgagcg tggccggcac
     9961 gttaaaccaa ctgcccgtat acgccccgcc ggcgatcggc ggagtgctcc atcggagcgg
    10021 caaacagacg ccgagcaggt tttgcagcaa gtcgttgaaa tagccggaaa agccgaccgc
    10081 tacggcggta ttgccgatcg cgtattccag aatcaggtcc cagccgatga tccaggcgaa
    10141 aatctcgcct aacgtggcat aggcgtaagt atacgcgctg cccgcaatcg ggatcatcga
    10201 cgccagctcg gcgtagcaca gggcggcgaa gccgcaggca atggcagtca gcaagtagga
    10261 aagggcgatt cccggaccgg cgccaggcct gcctatggtg gaaaccgccc tccggccgtg
    10321 cagcagcagg tcgagcaccg gcgccttcag aagggaaggt atcgcgacgg tctcgcccgc
    10381 cgccgccgtg cccgtgagga caaaaatacc ggttccgatc acggcgccca cgccgagcgc
    10441 cgtcaggctc cacggaccca gagatttccg cagccgcctc tcgggcgctt ccgcctgcgc
    10501 tacaagctgg tcgatgctct tggtctggag cagttgcgac acggccgtta ccgcttgcgc
    10561 tgtccggccg attgcctctt gattttgcgc ttgtgggatc ctctgggtac agtccgcttg
    10621 cccttcgggg gcgctttctt tcgcgccgcc cgtttcagtt tcttgcgttc agctttgtcg
    10681 gcaatctttt tggtgtccaa ttgagcgttt actcctggcg caatgtagcg tatttttcaa
    10741 tcagcttctt gatgccgtaa tgcaaaaaat ggacggtgat cttggcgtca tcgccctccc
    10801 cttcccgccg caacaccgtc ccagtgccgt atttcggatg atgcacccgg gtgccggcgc
    10861 gaaaatgcga agccttgctg cgcctcgccg gcgctccggc tggacctgcg ggcggggagg
    10921 ggtgtgcgcc ggctgcgacc gaggcgggcc cggtagcggc tgcccctcct gcgctgggcg
    10981 cagcctgcgg aaccggctgc ggcgcccgga tcccgcgagc cgcgaaaaat tcggcgatat
    11041 tttcgatgga attgtaagtt ttcccggtat atgcgctgcg ccgggccatc tgccgcacga
    11101 tctcctgctc cgcgtacaga tcgaggcccg gaatgacgcc cggctcggga gcgggagaaa
    11161 gatcctgaat cagcgagacg gggatctcgc tcaaaaaacg cgagggcagg ctcggctcca
    11221 gctctccgcc gccaaagcgg cgccggtatc gcgcgcgcgt caagataagc tgcttcctcg
    11281 cgcgcgtcat gcccacatag cagagccgcc gctcctcttc tatgccgctt tcggtttcgc
    11341 tggcgcgaat gtggggaaac aggatttctt ccatgccggc catgattacg agggagaatt
    11401 ccagcccttt cgcgctgtgc agggttagca gcgtgacctg agcgccctca tctagctggt
    11461 ccgtgtcggc caccagcgcg gcgtgatcca ggaattcgtt gacgccctcg ccgcgctcgg
    11521 cggcgtcggc cgccgcgttg atcagctcgt cgatgttttc catccgcgac tggctctcaa
    11581 cagtgtcttc ctcctcgagc atgcgttgat aacccgtccg ctgcttcaac cacaccagcg
    11641 tgtcgttcaa gcgctcactc tgaagcctcg acttcagctc atccatcagc ccgcaaaagg
    11701 cggtaaccgc ggcttgggcg cgagatggaa acgctccggt ccggagcagt tcttgcatcg
    11761 cgctccacaa agagatctcg cgctccaagg cgtatttttc gatctgttcg atcgttgatt
    11821 ttccgatgcc ccgggcgggc gtattgatga tccgcagcaa gctgaaagaa tcctgcgggg
    11881 acacggcggc tttcaaatag gccagcaggt ctttgatctc ggcgcgctgg tagaagctca
    11941 gcccgcccac cagcacatac cggcgcccgt agcgccgcaa tgcttcctcg atctgccgcg
    12001 attgaaagtt cgtccgaaac agcacggcca cgcggtcgcc cgggttttcc cgcagatatt
    12061 tctcgatcgt gtcggcgatg aaaagcgctt cattttccgc atcggccgcc gtatacagcc
    12121 agagcggcga ccccgccccg gcttcggtcc agagcttttt ccctttgcgc tccttgttgt
    12181 gagcgaccac atggccggcg gcgtccagaa tatttttcgt ggagcgatag ttctgctcca
    12241 gccggatcaa aacggctccc ggaaaatcgc gctcgaagtc caaaatgttg cggatatcgg
    12301 cgccgcgcca ggcatagatg gattggtctt cgtctccgac cacgcagatg ttgcgccgcg
    12361 gctcgcaaag cagccgcacc agctcgtact ggggccggtt ggtgtcctgg tattcgtcca
    12421 ccatgatgta gtggtatcgc tcggccgcgc gcagccgcgc gccttcgtcg tgccgcagca
    12481 ggcgcactgc ctcgagcagg agatcgtcga aatcgagggc gcccgtccgg cgcagagcgg
    12541 cttcgtattg ctcatacact acggccagct tttcagcttg agggttggtc gaacgccgga
    12601 aatattcttc gggcgtccag ccgtgatttt tggcatggct gatgcgcgag agtgcgcccc
    12661 ggtacgccaa aaatttttca tccaggccca actgtttata cacggctttg accgccgcga
    12721 gctgatcggc ttcatcgtaa atcgtgaagc gcgtcgtaaa gccggcccgc aaagcagcca
    12781 aaggcgcgcc gtcccgccgg agcaggcgga cacaaaagga atggaatgtc gaaaccgccg
    12841 ggctcacgcc ggcgagctcc gcgccgccgt tttcggcaag aagagccagc acgcggtctc
    12901 gcatctcgcc ggccgccttg ttcgtgaatg ttaccgccag gatgttgccc ggccacacgc
    12961 cccggtggca aatcaggtgc gcgatgcggt aggtgatcac gcgcgtcttg ccgctgccgg
    13021 cgccggcgag aatcaacagc ggcccttcgg tgtgctcgac agcctcccgc tgtttgggat
    13081 tcagttgttg gaggaaatcc atgctgaagc agacgaagcc tattttagca cgcggccccg
    13141 gggcttccgg ttgaggcggc tgtttgcgcg gctcgcgcgc cggattggcg acgggtctct
    13201 gcaagtttga gcgccgccgc cttgcggcac aaggcaaacc taatattccg gccaatcaat
    13261 cgccttccag accagccgga tcgcaaggcc caagactcac ggcacgggag cgaggacctg
    13321 aggcgattca tcgccgccgg cgttgaactc tctgccgcac actgggcggc acagatcaga
    13381 acgcgagggg atcgaaggat cgagtgggaa cagggccgag gcgcctgtcc ccacaggatc
    13441 actgcaacaa tccgccgtgg ccgaggctgg ccacctcgtg agcggcgact cggtagcggg
    13501 cgagaagggg cgggagtagc agatcaacga cgttaggctt aggggaacgg aaacagcctg
    13561 gagcagaaac aacggggaca tcgcccgcat aagccagcag cagaaggttg ccgggttcca
    13621 ccggcgccag gaaacgctcg agcttgcacc caatgcgctc catggctcgt cccaccacgt
    13681 cgcaggggcc tgccggagca gtggtggatg cgatcaagac aatcgtggga tgagaccgca
    13741 aaagatgatc cagcgcgcgg gcaacggccg gttcttcctc tatagctgta agcacgaagg
    13801 tgctgcccgt ccccaagcgc tgcaaacgaa ttctcatgat gctttcaaac tgttgccgcg
    13861 ctctgtcgcc ctgcaacaaa tccgagtaga gcacggcgac gaccgggtgg cggatcgggc
    13921 gcgcctgcaa gaccggaccg cactcgcgca gcatcgcgcg cgtcgcctcc atttcgcgct
    13981 ccggaatcgc gaaaggcgct gtcttgatcg tggccacgcg ctggcccgcc acagcatacg
    14041 agaaattcgg actggtcgcc accgccaggc agccagccga attcacttgc cgcaggagct
    14101 ggtcatccac cagcaggcag ctattttcag tggccaccag attcgcccgc ccgccggccg
    14161 ccagcttgat ctccaggctg ccacacccaa tttcagaagc catcatgctt gccgcgtagt
    14221 cctccggcac ctcgccctgc tcgagcgcag ccacccaaat gcgctctaat ccctccgcat
    14281 ccagcaagcg cacgtcctcc tcgctcaact gatggccctt ggccagcagt tttttcccgg
    14341 atggacggaa aatcggatga aacaacagcc ggccggctga atcctttacg gcaatggcct
    14401 ccatcttcat gatccacctc ctggaacacg caagtcgatt acaacccaga aatttttgtt
    14461 cgattatacg aaaatatcta gaaatagcaa ctgatttttt taatttattg tgaattataa
    14521 gaaatccgtg gtactagttt ggtactcgcc ccgataccgt ttgccggccg tttatttttc
    14581 tctggacgcc ttccttggcg atccccagcg gaataaaagc gatttttctc ccggacggcg
    14641 aaaagaggat tcgaaagagt gcagccagta cgactcgcgg agccggccgg ccggaactcg
    14701 ccgcggatgc aggctgccag tcccgcacct ccggtgtgca gcggttcctg atctaaaccg
    14761 gaagccgctg agccggcttg ccccttcccg ctgaagccag ctacgtgcca caggtcaaca
    14821 cgtcggagca aaaaatacaa aataaatgga cgaaaaaaat cgttatttca gtgtgcggcc
    14881 gaactaatgg cggcagcata cttgcagaaa aatcggccat ggcttctagc ccgggagcca
    14941 ggtctttccg agaaatgccg tccggcggca tcgccgcgcc gtgcgagccc ggccgatcct
    15001 ctggaggaaa gctcaggagg cggccagcga ggcggcgcat acgctagttc cgagcgggct
    15061 gcctgccagc ac
//
LOCUS       DASREK010000124        14107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1388304, whole genome shotgun sequence.
ACCESSION   DASREK010000124 DASREK010000000
VERSION     DASREK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388304"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFA54_06175"
     CDS             complement(<1..650)
                     /locus_tag="VFA54_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50426.1"
                     /translation="MNPPVRPPWHRRVAALLPLPLRRMLLRFEAAIEERVRQLALSLP
                     AGAKTLDAGAGEAQYARLFAHCCYIGVDLGVGDPGWDYSRLDARADLEKLPFPDACFA
                     AALNVTVLEHVREPARVLAEIARVLRPGAPFLLIVPQEWEVHQAPHDYFRYTRYGLEW
                     LLRQAGFVEVQIEPGGGFFTLLGRRLLDSVLFFQGGWRWMLFPLAAAVAGPAGLLLP"
     gene            complement(647..1615)
                     /locus_tag="VFA54_06180"
     CDS             complement(647..1615)
                     /locus_tag="VFA54_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS50427.1"
                     /translation="MIGKPWAGRNGILGCPRCRHSGFTVLFSATDRLYASTAREFQVV
                     ECGNCSLIRLEPQPSWEEIGAFYPENYWWAPDRSVIGRLEGIYRRIVLSDHVRFVEHG
                     LERGALVLDVGCGGGSFLSALRRRGMRGVGLDFSAGAAAVAWRSQGVPALCGLLESSP
                     FAPESFGGITMFHVLEHVPDPSESLAAARRLLSPAGKLYVQVPNAACWQFLLLGDRWS
                     GIDVPRHLIDFRKTDVEQLLEETGFVVRREKFFSLRDNPAGLATSLAPGLEPMSRRAR
                     RVRESGARRLLKNLVYLALSAAAVPFTALEAAAGAGSTVLIEAVKK"
     gene            complement(1612..2649)
                     /locus_tag="VFA54_06185"
     CDS             complement(1612..2649)
                     /locus_tag="VFA54_06185"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS50428.1"
                     /translation="MTAFVQNPLVSVVVVNWDRKDLLRACLESLSRQSFVNFETIVVD
                     NGSRDGSAAMVAAEFPGVRLIENSENRGFCAANNQGIAAAAGRYIALLNNDAEADPRW
                     LEELLQVFELGADIGMAASKILVWENRQTIDKAGHLIYPDGQNRGRGTGETDRGQYDR
                     LEETSWPDGCAAMYRKDMLDQIGGFEEDFFAYADDAELGLRARIAGWRCLYAPAAVVY
                     HHQGSTLGRFSPERLILIERNRVWLAAKLFPWRLLLLNPFYFLLRVAAGVYASQRGSG
                     EAALFTSRRGFPVLVKCFFRANLAAFAGLPRMLAKRRDVARIRRLSASEVCALLERFR
                     IRLRDLTLKTS"
     gene            2725..3852
                     /locus_tag="VFA54_06190"
     CDS             2725..3852
                     /locus_tag="VFA54_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZS50429.1"
                     /translation="MGDFGQNLRREREARGVALEDISRATKISVRLLQAIEDEEFERL
                     PGGIFNVNFVRQYARQLGLDDERIVSEFRRLTAPPVETETPRSSLPPEWIARAAEQRA
                     REREPRVWMAATVVVAAIAIGGAAYLIWSGRRAPALERLSPPAASAPTPAAPAGIPEP
                     TRPEGLPADSAQSLSSRPSAPEEHAPAVERPASAAPQNPASPTAAAIQSRKPAPAAGA
                     APIHEQTASSPTQPAALSSGVTPAADVSSETPAETVWAEAPVRVEIQATSIVWVGASA
                     DGQPRFQTTLRAQQTRRIAAQSSVRLRVGDAGALMVTLNGQPQPPIGPKGQVRTVILT
                     AQGMRVLAPPSAAPPSEGARPADTTEIQQGAGGAAEAGPNP"
     gene            3875..4987
                     /locus_tag="VFA54_06195"
     CDS             3875..4987
                     /locus_tag="VFA54_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZS50430.1"
                     /translation="MPRRIYLDYNASTPIAPEVEEAMRPFFSEHFGNPSSAHWAGRSA
                     REAVETARAEVAALIGCSAEEIIFTSGGSEANNHALKGVFFARGGAGGHIITTQIEHP
                     AVLNPCRFLEQLGAKVTRVPVDRFGRVDPDDVRRAIIPGTILISVMHANNEVGTIQPL
                     REISRIAREHEILFHTDAAQSCGKIPVRAGEFGVDLLSVAGHKLYAPKGVGALFLRRG
                     VAIEPLIHGAAHESGRRAGTENVLLDVALGAACRLAQSWIGRPHIQDLRDLFWSLLQK
                     RFGECVVLNGHPAERLPNTLNVSFVGRIGAEILARMENIAASTGSACHAGSIEISPVL
                     KAMGTPLSVAAGAIRFSLGRNTTREEIEEVAATLEI"
     gene            complement(5093..6172)
                     /locus_tag="VFA54_06200"
     CDS             complement(5093..6172)
                     /locus_tag="VFA54_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HZS50431.1"
                     /translation="MKGITVKILRHILVAAVLVFMPISMRAQQAQPKAQRKAGGQAQA
                     EPSKPPAEPVQFPDKNLEAAVRQQVIGKKDPSQPLTKDDVRYVATIEAKGKGIRDLTG
                     LENCVSLTSLDLSGNEITDLTSLEKLKSVEKPDTLEVGSQIKWVDLSKNKITSLKGLD
                     ALGKLEYAQLSDNQITDLTPLGSLTRLNDLYLSNNQIKDLTPLANLKKLWTLYADGNQ
                     ITDLKPLAGLTQLTSLDLKGNQITDISPLAALTSLRYLALDGNKIHTLAPLVSSAKKD
                     FAGDKSFAPYLKLYVARNPLTSAAKTAQVAELKSYGVTVIDGGMTPSAAPKTGTRPGA
                     SGPRPKAVAKSSASSKTSASAKALK"
     gene            6423..6499
                     /locus_tag="VFA54_06205"
     tRNA            6423..6499
                     /locus_tag="VFA54_06205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6457..6459,aa:Arg,seq:cct)
     gene            complement(6508..6780)
                     /locus_tag="VFA54_06210"
     CDS             complement(6508..6780)
                     /locus_tag="VFA54_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50432.1"
                     /translation="MSSSLAIAGIIEMQRSYKTLSLPPPPPAQPEDDLIGWMGLPELA
                     EFAPAVSDGQGGDDVLRAVAAQAAARRESGRRSLFSRLFSFRFFRR"
     gene            7034..7120
                     /locus_tag="VFA54_06215"
     tRNA            7034..7120
                     /locus_tag="VFA54_06215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7068..7070,aa:Leu,seq:tag)
     gene            7343..8557
                     /locus_tag="VFA54_06220"
     CDS             7343..8557
                     /locus_tag="VFA54_06220"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZS50433.1"
                     /translation="MGSDADSRSGPILVLSGTKRVVITIARSLARHGIPVHTAGFDSS
                     GGSPTRSRFIQRHFSLPDFRRNPDLFRERLLALIDRYSYKTLLPAGDDALVSLGPVDA
                     ELRRRVRLLCPPPDIAGLVLDKPKSLALARECGLRTPAQFCVDGQRLEDFRHILPFPL
                     VAKPANKARNQQPGSRRIEDFEDLRQTILANPRFCEEWVLQEYCSGFGVGVEVLLWDN
                     EPLIVFQHRRVKELPSSGGVSVKCAAEPPDPELAEGSVRLLRRLGWQGVAMVEYRFDP
                     ATRSAVFMEINGRFWGSLSLPVHAGVDFPYYLWELAHQRRPQMPARYPPGVCVMWRAG
                     DCLRLLGLLQEWRAGRFPLSKLWREIGRFGADFFSPVTDAVWSLSDPWPAISEFFSLL
                     RLLPFAAHSRER"
     gene            8746..9411
                     /locus_tag="VFA54_06225"
     CDS             8746..9411
                     /locus_tag="VFA54_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZS50434.1"
                     /translation="MVEAAFGRAASHRLRDEIVLLYAITDRSQLTAPLSERIALLLQA
                     GVDFLQIREKDLSPRELFEVVKSAVALPNPHGTRILVNERTDVALAAGAHGVHLPAGS
                     ISPARIRRVVPAGFLIGSSCHSAEEVAFAEAAGADFAVFGPVFETPSKRSYGPPLGTN
                     ALAEAVQGRRIPVLALGGVTPQNFLQCRAAAGIAAISMFQNAADPGGLVKTLRARWQE
                     GGS"
     gene            complement(9428..10177)
                     /locus_tag="VFA54_06230"
     CDS             complement(9428..10177)
                     /locus_tag="VFA54_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50435.1"
                     /translation="MSGSISLASRLPVEEFAEQFQREMLPLNNTYSYFAALPLDDHQV
                     QQYVQEPVAALPPSLSEILPKVRLVLVPYLERSNGKDLVAFEKPAEARQVPAASLELG
                     GEIFFFLAVKDEEIADYHYTFYNGLASLASFRLTEAQRERFSRLLREELANEAHGEVE
                     EKSWRLKQQLLRRQTNLLRDTKLQREYFRQAFEDTATLYLHGLCCDIDVETGPRQLPT
                     RYLRKRLELLRQTFPPPEGFALFPEELGRRE"
     gene            10239..11420
                     /gene="lpxB"
                     /locus_tag="VFA54_06235"
     CDS             10239..11420
                     /gene="lpxB"
                     /locus_tag="VFA54_06235"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HZS50436.1"
                     /translation="MKVFISAGEDSGDQYGAALVSVLQRRWESSVFFGCAGPRLRAAG
                     VHPVVSAERLAVVGLVEVAGHLPRIYRDFRRLYRAARQERPDLAILVDSPDFHLRLAA
                     KLARLQIPVVYLIAPQAWAWRQWRVRQMRRVITRLLCIFPFEEEFFNKRGVPAVYIGH
                     PLAEAARPSLSRHDFFRKHRLASERPLIAVLPGSRQGEAARHLPLLLDAIRQLNTWRA
                     MTFVVAASRSTGKKFYARYAAGSGVPVIEGDTHNLLAHCDLALVASGTATVEAALLGA
                     PMVTFYRVHPATWILGKWLVDAPFLSMVNLLAGRKIVPELIQSEFTSARLAEEARALL
                     DGPEHAGQMRRALAELAGRLDTGSGVMERAADVCEEVLTAARRVPPQVRATAQNEVRS
                     W"
     gene            11420..13390
                     /locus_tag="VFA54_06240"
     CDS             11420..13390
                     /locus_tag="VFA54_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZS50437.1"
                     /translation="MVRRSLLSVLIALGLLAPASAQDRRPRIDVEHYRIDAEIHPNTQ
                     SLTARVLVQFTPQEDTSSAIFELNNGLNVTRVSGEKNEEIPATRYQQDFTVRLNFPAT
                     LPKGKPVAVTFQYDGRLMGLDNSPVEGISFASIAGDRAYLLYPARWFPVSGYTSDRYT
                     AELNITVPAGMDVIASGLHTTKPAPEGKVIHSFTYDHPSFPGSVGVVTGQPERVSSEG
                     ITTTLYFRGPEKEQASAYGDAAGKIVRYFSSLFDLPPSANLTVLETEDNAPNGYAAPG
                     ILFLSPRGIGKKLNSRLLSLEIAHQWWGILVSPASRRHLWLENGFANYAHLLWVENSA
                     GPAAFNAEVQDTSIEALTNEETPVIQADTLPDYSPELMAITSAKGAMVLHMLRYLVGD
                     DVFFRSVKEFAQKYAWNSAITADFEKIVSAEAKQGLQYFFIEWIESSGASEFKLEYTI
                     FRLGEGKGFRVVGKVTQDKDTFRMPVEMLIETEGEPERKKIEVAGTSSEFSVDTFGKP
                     KRVILDPDHKVLRLDDKIRVAVAIKKGEQQAELGYYNEALQEYQKALDVNRNSSLAHY
                     RIAEVFFLQRNYQSAANEFREAINGDGEPKWTEVWSHINLGKIFDITDQRDRAVNEYQ
                     QAIRTKDNTQNALDEANKYLQSPYRRPSRSEV"
     gene            complement(13401..13628)
                     /locus_tag="VFA54_06245"
     CDS             complement(13401..13628)
                     /locus_tag="VFA54_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50438.1"
                     /translation="MRVRVESYSGYKADESPLRFLLGEHRYSVVEIIDRWYGPGYRYF
                     RVRADDGNLYLLRFDEVTQEWSLAGYREEEG"
     gene            complement(13631..>14107)
                     /locus_tag="VFA54_06250"
     CDS             complement(13631..>14107)
                     /locus_tag="VFA54_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50439.1"
                     /translation="SAAPPALPEPEFPKPPGDREIAKPLAKRPRRTSSPGEPPMHEGA
                     TGRALSPPSGFCFLFLLFRGYRLEVLCLENFFTVEAFDVVHIAISGNDFGARVLAVRL
                     HIDGLSFPVKCGCYEEVVASSMRDEHTDRPYFIHPFLIVKRNGFEKMHPKPLAREN"
BASE COUNT         2722 a         4394 c         4300 g         2691 t
ORIGIN      
        1 ggcaacaaga ggcccgccgg cccggcgacc gccgccgcca gcggaaacag catccaacgc
       61 cagccccctt gaaagaaaag caccgagtcg agcaggcggc gtcccagcaa ggtgaagaag
      121 ccgccgcccg gctcgatctg cacttcgacg aaaccggcct ggcgcagcag ccattccagg
      181 ccatagcggg tataccgaaa atagtcgtgc ggcgcctggt gcacctccca ctcctgggga
      241 acgatgagca gaaatggtgc gccggggcgc aatacgcgcg cgatttccgc cagcacccgg
      301 gccggctccc ggacgtgctc caaaacagtc acattcagcg ctgccgcgaa gcaagcgtcc
      361 ggaaaaggaa gcttctccag atcggcccgg gcatcgagcc gggaataatc ccaccctggg
      421 tctcccacgc ccagatccac gcctatgtag caacaatgtg caaaaagccg ggcatattgc
      481 gcttcgccgg ctccggcatc gagcgtcttg gcgccggccg gcagcgacag agccaattgg
      541 cggactctct cctctatggc cgcctcgaag cgcaacagca ttcggcgaag cggcagaggc
      601 agcaaggcag ccactcgccg gtgccagggc ggccgcaccg gggggttcac ttctttaccg
      661 cctcgatgag gacggtggat ccggccccgg cagctgcttc gagcgccgta aacggcacgg
      721 cggccgcgga aagagccaga tatacgagat tcttcagcaa tcggcgcgcg cccgattcgc
      781 gcacccgccg cgcccgccgg gacatcggct cgagccccgg cgccagactt gtcgccaggc
      841 ctgcgggatt gtcgcgcaga gaaaaaaatt tctcccgccg gacgacaaat ccggtttcct
      901 ccagcaactg ttccacatcc gttttccgga aatcgatcag gtgccgtggc acgtcgatgc
      961 cgctccaccg gtcgccgagc aacagaaact gccagcaagc ggcgttggga acctggacgt
     1021 aaagcttccc ggccggcgac agcaagcggc gggcggcggc gagggattcg gagggatccg
     1081 gcacatgctc caggacgtga aacatggtaa tcccgccaaa gctctccggc gcgaagggcg
     1141 agctctccag cagcccgcag agggcgggaa ctccctggct gcgccaggct acggcagcgg
     1201 cgccggcgga aaaatccaag cctactccgc gcatgccgcg gcggcggagg gcggaaagaa
     1261 aggacccgcc gccgcagccc acgtccaaga ccaatgcgcc gcgctcgagc ccgtgctcta
     1321 caaaacgcac atggtcgctc agcacgatcc gccggtagat gccctccagc cggccgataa
     1381 ccgagcgatc cggagcccac cagtaattct ccggataaaa tgcgccgatc tcttcccacg
     1441 acggctgcgg ctccagccgg atcagcgagc agttcccgca ttccacaacc tggaactcgc
     1501 gcgcggttga agcatacagg cggtccgtgg ccgagaacaa aacggtgaag ccgctgtgcc
     1561 ggcagcgggg acagccgaga attccgttgc gcccggccca aggcttcccg atcatgaagt
     1621 tttcagggtc agatcgcgca ggcgaattcg gaaccgttcc agaagagcgc acacctcaga
     1681 ggctgaaagc cgccggattc gcgccacgtc ccgccttttg gcgagcatgc gaggaagccc
     1741 agcgaaagca gcgagattgg cgcgaaagaa acattttacc agcaccggga atccccgccg
     1801 gcttgtaaac agcgccgctt cgcccgaccc ccgttgggat gcgtaaacgc cggccgccac
     1861 gcgcagcaaa aaataaaacg gattcagaag cagcaggcgc caggggaaca gtttagccgc
     1921 cagccacacc cggttgcgct cgatgagaat caaccgttcc ggcgaaaaac ggccgagagt
     1981 cgagccctga tgatgataga ccacggcggc cggcgcatac aggcagcgcc agccggcgat
     2041 ccgcgcgcgc aagcccagct cggcgtcgtc ggcgtaggcg aaaaagtcct cttcgaaccc
     2101 gccgatctgg tccagcatat ctttccgata catagcagcg cagccgtcgg gccacgacgt
     2161 ttcctcgagc cggtcgtact gtccccggtc ggtctccccg gttccgcggc cccggttttg
     2221 gccgtcggga tagatcaagt ggccggcctt atcgatggtc tgccggtttt cccaaaccag
     2281 aattttggaa gccgccatcc cgatatccgc tcccagctcg aacacctgca acagttcttc
     2341 cagccagcgc ggatcggctt ccgcgtcatt attcagcaga gcgatatagc gccctgcggc
     2401 cgccgcaatg ccctgattgt tggccgcgca gaagccccga ttttcggaat tctcgatcag
     2461 ccgcacaccg ggaaattcgg cggcgaccat ggccgcagaa ccatcccggg agccgttatc
     2521 caccacaatg gtctcgaagt tcacaaagct ctggcgagaa agcgactcga ggcaggctcg
     2581 cagcagatct ttgcggtccc agttcaccac gacaaccgac accaatggat tctgtacaaa
     2641 agccgtcact ggatcccatc tgcggtcgcg gatactcggc agggctgtag tattataaac
     2701 ccgaagagtc ttttcccaga aaaaatgggc gacttcgggc agaaccttcg gcgtgagcgc
     2761 gaagcccgcg gcgtagccct cgaggatata tcccgggcca cgaaaatcag cgtgcgcctt
     2821 ttgcaagcca tcgaggacga ggaattcgaa aggctgccgg gcggaatttt caacgtcaac
     2881 ttcgtgcgcc agtatgcgcg ccagctcggg ctggatgacg aaaggatcgt cagcgaattt
     2941 cgccggctca cggcgccgcc ggttgaaacc gaaacgccgc gttcctctct gcccccggag
     3001 tggattgcgc gcgcggcgga gcagcgggct cgggagcgcg agccacgcgt ctggatggcg
     3061 gcgactgtcg tagtggcggc catcgcgatc ggcggcgccg cgtatctgat ttggtcaggc
     3121 cggcgggcgc ccgcgctgga gagactctcg ccgccggctg cctccgcacc gacgccggcg
     3181 gctcccgccg gtattcccga gccgacccgc ccggagggcc tgccagcaga cagtgcgcaa
     3241 agcctgtcca gccggccttc tgcgccggaa gagcacgccc cggcggtgga acgcccggcg
     3301 agcgcggcgc cgcaaaatcc ggcttcgccc acggccgcgg cgatccagtc ccgaaagccc
     3361 gcgccggcgg ctggggcggc gcccattcac gagcagacgg cgtcttcccc gacacagccg
     3421 gcggctctca gttccggcgt cacgccggct gccgatgtca gttcggagac gcctgcggag
     3481 accgtctggg ccgaggcgcc cgtgcgtgtg gaaatccagg ctaccagcat cgtgtgggtt
     3541 ggggcctcgg cggacggcca gccccgcttc cagacgactc tgcgcgctca acagacacgc
     3601 cggatcgccg cgcagtcatc ggtacggctc cgggttggtg atgcgggagc gctcatggtc
     3661 accctcaatg gccagccgca gcctcccatc ggcccgaaag gccaggtgcg cacggtgata
     3721 ctcacggcgc aaggcatgcg cgtcctcgct cctccttctg cggcgccccc gtccgagggc
     3781 gcccgccccg ctgacacgac ggagatccag cagggagcag gaggagcagc tgaagccggt
     3841 ccgaatccct gaccgggacg gccccgcgct gcttatgccc cgccgcattt atctcgatta
     3901 caacgccagc accccgatcg caccggaagt agaggaggcg atgcgcccct tcttctccga
     3961 gcatttcggc aacccctcca gcgcgcattg ggccggccgc tccgcgcggg aagcggtcga
     4021 aaccgcccgg gctgaagtcg ccgccttaat aggctgctcg gcggaggaaa tcattttcac
     4081 cagcggcggc agcgaggcga ataaccacgc tttgaaaggc gtgtttttcg ccagaggagg
     4141 cgccggcgga cacattatta ccactcagat cgagcacccg gctgtgctga acccgtgccg
     4201 ctttctggag cagctcggcg ccaaagtcac gcgtgtgccg gtggaccgat tcggccgcgt
     4261 ggaccctgac gatgtgcggc gggccatcat tcccggcacg atcctgattt cggtgatgca
     4321 cgccaacaac gaagtgggaa ccatccagcc gcttcgcgag atctcacgaa tcgcccgcga
     4381 gcatgaaatc ctgtttcaca ccgacgcagc gcaatcctgc ggcaaaattc cggtaagagc
     4441 cggcgaattc ggcgtggacc tgctttctgt ggcgggtcac aaactctacg ctccgaaggg
     4501 cgtgggagcg ctgtttctgc gccggggagt tgccattgag ccgttgattc acggggctgc
     4561 gcacgaatcg ggacgccgcg ccggcaccga aaacgtgctg ctggacgtgg ctttgggcgc
     4621 agcctgccgg ctggctcagt cctggatcgg ccggccgcac atacaggatc tccgggatct
     4681 gttctggagt ctcctgcaaa aacggtttgg cgagtgcgtt gtgctcaacg ggcatccggc
     4741 cgagcggctg ccgaataccc tcaatgtgag ttttgtcggg agaatcgggg cggagattct
     4801 ggcccggatg gaaaatatcg cggcatctac cggttcggcc tgccatgcgg gctcgataga
     4861 aatctcccct gtcttaaagg cgatggggac gccgctctcg gtggctgccg gcgcgattcg
     4921 tttcagcctt ggccgtaaca cgacccgcga ggagatagag gaggtcgcag ccactctcga
     4981 gatctaacga agtttccgaa ttcgccacgc cagatccgct ccagccgcgg cgaagcggga
     5041 ttttcccgtg accgggaaat tctcttgcgc cggccgtgat ccccgcttcg gcctacttca
     5101 acgctttcgc gctggcgctg gtcttgctcg aggcagatga cttggcgacg gccttgggcc
     5161 gcgggcctga agcgccgggg cgtgtccccg tcttcggcgc tgccgaggga gtcatgccgc
     5221 cgtctatcac ggtcacccca tagctcttga gctcggccac ttgggcggtc ttcgccgccg
     5281 aagtgagcgg attgcgcgcc acgtaaagct tcagataggg cgcaaagctt ttgtcgcccg
     5341 caaaatcctt ctttgccgag ctgaccagag gagcgagagt gtggatcttg tttccgtcga
     5401 gcgctaagta gcgcagtgaa gtaagcgcag caagcggcga aatatcggtg atctgatttc
     5461 ctttcagatc caacgaagtg agctgcgtca aaccggccag cggtttcaga tcggtgatct
     5521 gattgccgtc ggcgtaaagg gtccagagtt ttttcaggtt ggcgagcggg gttaggtctt
     5581 tgatctggtt gtttgaaagg tacagatcat tcaaacgcgt caggctgccc agcggcgtta
     5641 gatcggtgat ctggttgtcc gaaagctgag cgtactccag tttcccaagc gcgtcgaggc
     5701 ctttcaggct ggtaattttg tttttggaaa gatcgaccca cttgatctgg cttccgactt
     5761 ccagcgtgtc cggtttctcc acgcttttca gtttctcaag cgacgtcaga tcggtgattt
     5821 cgtttccgga taagtccagc gacgtgaggc tgacgcagtt ctccagcccg gtcaagtccc
     5881 gtatgccttt gcctttggcc tcaatagtgg ccacatagcg gacatcgtct ttcgtgagag
     5941 gctggctagg atcttttttt ccgatcacct gctggcgcac ggcggcttcc aaattcttgt
     6001 ccggaaactg gacgggctcc gccggaggct ttgacggctc cgcctgcgcc tgcccgccgg
     6061 cctttcgttg cgctttcggc tgcgcctgct gggcccgcat ggaaatgggc atgaacacta
     6121 aaacggctgc aacgagaatg tgacgcagaa tcttcaccgt gattcccttc atacgacagc
     6181 tcctggttgt aagctgcatc gcaaccgcga tgtgggtgta atgctgcgtg agttagcaat
     6241 ctattgtact caatcccaaa ccccgcggcg gtggaaacct cggatcccgt ccgagcggcg
     6301 cggccgtctc gcccacccgg cggcgcctgg cagggacacg gcggatgtgg gagaggcacg
     6361 gataccgccc gttctcgacc cgcaaggaag ttcactggcg gtgaggtaaa atgaagcggc
     6421 aagggcacgt agctcagatg gatagagcgg tcgcctccta agcgacaggt cggcggttcg
     6481 aatccgcccg tgcctaccac ttccctttca gcggcggaaa aatcggaaag aaaaaagtct
     6541 ggaaaacagc gatcgccggc ctgactcccg gcgggccgca gcctgggcgg cgacggctcg
     6601 cagcacatca tcgccgccct ggccatcgct caccgcgggc gcgaactctg ccaattcggg
     6661 cagccccatc cagccaataa ggtcatcttc tggctgggcc ggcggcggag gaggaagact
     6721 cagggtttta tagctccgtt gcatttctat aattccggca atggcgagac tggaactcat
     6781 aagatgttcc cgtttctaaa atactgggat ggcggtgaaa ttactatggt acgagcctct
     6841 cattctttgc ccctacggat gtctcatccg cacccgtgag actcccgacc atccagtgaa
     6901 ccgatcgtcc taccccatag gtttattccc ttagctgccg tctttttgac gccgcgcgcc
     6961 gcagccccgc cctcttgcga gcgccggctg atttacgacc tgagcggtgt aaactgaatg
     7021 ggagacctga tttgcgaacg tggcggaatt ggcagacgca ccagacttag gatctggcgg
     7081 ccgcaaggcc atgggagttc gaccctcccc gttcgcacca gttttgcagg aatcgggttc
     7141 ttgcccggcc ggctctcggc tgcgggagat cctactgtgg tcgaaatttg gcgattgtcg
     7201 cggaaaatgc cctgcaattt cgcccggaat cggccggggc gtgcttttgg acgaagttga
     7261 cggggtttca cagaggccct acaacaacga tcggctgccg ggaaagagtc caaaatcttg
     7321 ccaggcactc tgcccatgag gcatgggaag cgacgctgat tctcgttccg gtcccattct
     7381 ggttctttca ggcacgaagc gggtagttat aaccatcgcc cggtcgcttg cccggcacgg
     7441 cattccggtt catactgccg gcttcgactc ctcgggtgga agtccaacgc gttctcgctt
     7501 catacagcgc cacttcagcc ttcccgactt ccggagaaat cccgacttat tccgcgaacg
     7561 ccttttggcc ttgattgatc gctattcgta caaaactctc ttgccggccg gcgacgatgc
     7621 actggtttcg ctggggccgg tcgacgccga gcttcgccgc cgcgtcaggt tgctttgtcc
     7681 tccgccggac atcgccggcc tcgtgctgga caaaccgaaa agtttggcct tagcccgtga
     7741 gtgcgggctt cgaacgccgg cccaattttg cgtcgatgga cagcgtttgg aggatttccg
     7801 ccatattctg ccatttcctc tcgttgccaa accggcaaac aaagctcgga atcagcagcc
     7861 cggcagccgc cgcattgaag atttcgaaga tctgcgccaa acgattctgg ccaacccgcg
     7921 tttttgcgag gaatgggtat tacaagaata ttgctccggc ttcggcgtag gcgttgaagt
     7981 actgctgtgg gacaacgaac ctctcattgt gttccagcac aggcgggtga aggaactgcc
     8041 cagttccgga ggagtgagtg tgaagtgcgc agccgagccg cccgatccgg aactggcgga
     8101 aggctcggtg cggctgcttc ggaggctcgg gtggcaaggc gtggccatgg tcgaataccg
     8161 cttcgatccc gcgacgaggt cagcggtgtt catggagatt aacggccgat tttgggggtc
     8221 tttgtcgctg cctgtgcatg ccggcgttga ttttccctac tatctttggg agctcgcgca
     8281 tcagcgccgg ccccagatgc ccgcccgtta tccacccggc gtctgcgtga tgtggagggc
     8341 gggcgactgc ctgcggctgc tcggcttgct tcaggagtgg agagccggca gattccctct
     8401 ttccaaacta tggcgcgaga taggccgctt cggggccgac tttttctccc ctgtaacgga
     8461 tgccgtgtgg tcgctttccg atccctggcc ggccatttcc gagttctttt ctctgctccg
     8521 tctcttgccc tttgcggcac acagccgcga gaggtagcga ctccaggaac cgctgcggcg
     8581 cggtatccgc gtccggtccc gccggcccgc cgtcttcttt gtttcctgca taacccggct
     8641 ctctggctgg ttcgcgcgaa aagagatcgg cagggcttgt gccttgctgc gcagccacag
     8701 gcccgcttgc aactcatgcc gccggccggc gtaaagtgaa ggagagtggt cgaggccgct
     8761 ttcggccgcg ccgcaagcca cagacttcga gacgaaatcg tgcttcttta cgccattacc
     8821 gaccgctcgc aattgacggc gccgttgagc gagcgtatcg cccttttgct ccaggcgggc
     8881 gtcgattttc ttcagatccg ggaaaaggat ctctcgcctc gcgaactgtt cgaagtggtt
     8941 aaatcggccg ttgctcttcc taatccgcac ggaacaagga ttctggtgaa cgagcgcacc
     9001 gatgtcgctc tggctgccgg cgctcacggc gtgcatctgc ccgctggatc gatctccccc
     9061 gcgcggattc gccgggtcgt cccggcgggc ttcctgatcg gcagttcctg tcactcggcg
     9121 gaggaagttg cttttgcgga ggcggcgggc gccgacttcg ccgtattcgg gcccgttttt
     9181 gaaacgcctt ccaaacgaag ctacggcccg ccgctcggga ccaacgctct ggcggaagcg
     9241 gtgcagggcc gccggattcc ggtactcgcc ctaggaggcg ttaccccgca gaacttcctt
     9301 caatgccggg cggcggcggg aatcgcggca atatccatgt ttcaaaacgc tgccgatcca
     9361 gggggcttgg tgaaaacttt gcgcgcgcgg tggcaggagg gaggatcctg aagcgccatt
     9421 ctgaaggtta ttcgcgtctt cccaattctt ccgggaagag cgcgaagcct tcaggcggag
     9481 gaaaagtctg gcgcagcagt tccagccgct tccgcagata gcgcgtgggc aactgtcgcg
     9541 ggccggtttc cacgtctata tcgcagcaca ggccatggag ataaagagtg gccgtgtctt
     9601 cgaacgcctg gcgaaagtac tcccgctgca actttgtatc gcgcagcaga ttcgtttgcc
     9661 ggcgcagcaa ctgctgcttc agacgccagc tcttttcttc cacttcccca tgcgcctcgt
     9721 tggccaactc ttcccgcagg agccggctga accgctcgcg ctgcgcctcg gtgagccgga
     9781 aggaggcaag cgaggcaagg ccgttgtaaa aggtgtaatg atagtcggcg atttcctcgt
     9841 ctttgactgc cagaaagaaa aaaatctcac cccccagctc gaggctcgcc gccggcacct
     9901 ggcgcgcctc cgccggcttc tcgaacgcca ccagatcctt tccgttgctc cgctccagat
     9961 aggggaccag caccaggcgc actttcggta aaatttcgct taacgacggc ggcagggccg
    10021 ctactggctc ctgcacgtat tgctggacct gatggtcgtc gagcggcagc gccgcgaagt
    10081 acgaataggt gttattgaga gggagcatct cgcgctggaa ctgctcggcg aactcctcca
    10141 ccggcaagcg cgacgccaag gaaatagaac cagacatgtc ctgctgccat cgtagcacag
    10201 cgatgcatcc gcagcgccgc attctatact ggagtcaaat gaaggtgttc atctcagcgg
    10261 gggaagactc cggcgatcag tacggagcgg cactcgtctc tgttcttcag cggcggtggg
    10321 aatcctcggt cttctttggc tgcgccggac cgcggctgcg tgcggcgggc gttcatcccg
    10381 tggtcagtgc agagcgtctc gccgtagtcg gactggtcga agtcgctgga cacctgccgc
    10441 gcatttacag ggattttcgc cgcctatacc gcgccgcccg gcaggagcgg cccgatctgg
    10501 cgatactcgt tgactcgccg gacttccacc tgcggctggc ggccaagctc gcccggctgc
    10561 aaatcccggt ggtctacctg atcgctccgc aagcctgggc gtggaggcaa tggcgggtaa
    10621 ggcagatgcg ccgcgtgatt accagactgc tctgcatttt tcctttcgag gaagaatttt
    10681 ttaacaagcg gggcgtcccg gcggtctata tcggccatcc gctggcggag gctgcgcggc
    10741 cctctttgtc gcgccacgat ttttttcgga aacacaggct tgcttcggag cgtcccctta
    10801 tagcggtgct cccgggcagc cgccagggcg aagccgcgcg gcacctgccc ctgttattgg
    10861 acgcgatccg gcagctaaac acatggaggg ccatgacgtt tgttgtggcc gcctcccggt
    10921 ctacgggaaa gaagttttat gcccggtacg cggccgggtc gggcgtcccg gtgatcgagg
    10981 gcgacacgca caacttgctg gcccattgcg atctggctct ggtggccagc ggcaccgcga
    11041 ccgtcgaggc cgcactgctg ggcgcgccta tggtgacttt ttacagggtt catcccgcaa
    11101 cctggatttt gggaaaatgg ctggtggatg ctcccttcct gagtatggtc aacttgctcg
    11161 ccgggcgtaa aatcgtgccc gaattgattc agagcgaatt tacctccgcg cgcctggccg
    11221 aggaggcgcg cgcgctgctt gacggtccgg agcacgccgg acaaatgcgc cgtgcgctgg
    11281 cggaactggc agggcgactg gatacaggga gcggcgtgat ggagcgcgcg gctgacgttt
    11341 gcgaagaggt tttgaccgct gcccgccggg tccccccgca agtgcgcgcc acggcgcaaa
    11401 atgaggtgag gagctggtga tggttcgcag gtcgttgctt tccgttctta tcgcgctggg
    11461 cctgttggcg ccggcttcgg cccaggatcg gcggccgcgc attgatgtcg agcactaccg
    11521 gatcgacgcc gagattcatc cgaacacgca gtctttgacg gcgcgggttc ttgtacagtt
    11581 cacgccccag gaggacacct cgagcgccat cttcgagctc aacaacggcc tcaatgtcac
    11641 gcgcgtgtcc ggcgagaaaa acgaagaaat ccccgctaca cgctaccagc aggacttcac
    11701 cgtccggctc aactttccgg ccacgctgcc caaagggaag cccgtggctg ttacgtttca
    11761 gtacgacggc cggcttatgg gtctggacaa ctcgccggtc gaaggcatca gtttcgcctc
    11821 tatcgcgggg gaccgcgcgt atctcctcta tccggcccgc tggttcccgg tcagcgggta
    11881 taccagcgac cggtataccg ctgaactgaa tatcaccgtt cccgccggaa tggacgtgat
    11941 tgccagcggc ttgcacacca ccaagcccgc gccggaaggt aaagttatac atagcttcac
    12001 ctacgatcac ccttcctttc ccgggagcgt tggcgtggtg accgggcagc ccgagcgcgt
    12061 atcgagcgaa ggcattacta ctactctcta cttccggggg ccggagaagg agcaggccag
    12121 cgcttacggc gacgcagccg gcaagatcgt aaggtatttc agcagcctgt ttgatctgcc
    12181 cccttccgcc aatttaaccg tccttgagac cgaagacaat gcgccgaacg gctatgcggc
    12241 gcctggaatt ctcttcttga gcccccgcgg catcggcaaa aagctgaaca gccggctgct
    12301 cagcctggaa atcgcgcatc agtggtgggg aatactggtc tcaccggcgt cgcggcgcca
    12361 cctctggctc gaaaacggtt tcgccaacta cgctcatctt ctctgggtgg agaacagcgc
    12421 cgggccggcg gcattcaacg ccgaggtgca agataccagc atcgaggcgc tcaccaatga
    12481 agagacgccg gtcatccagg ctgatacgct gccggattac tctccggaac tcatggcgat
    12541 aacctcggcc aagggcgcga tggtgctcca tatgctccga tacctggtcg gggacgacgt
    12601 gtttttccgc tcggtgaaag agttcgctca gaagtatgcc tggaattcgg cgatcacggc
    12661 ggacttcgag aaaatcgtgt ctgccgaggc caagcagggc cttcaatact ttttcatcga
    12721 atggatcgaa tccagcgggg cctcggagtt caagctcgag tacacgatat tccgcctcgg
    12781 cgaaggcaaa ggcttccgcg tggtcgggaa ggtgacgcaa gataaggaca ccttccgcat
    12841 gccagtggag atgctcattg aaaccgaggg tgagccggag aggaagaaga tcgaggtggc
    12901 gggcacgtct tcggaattct cggtcgatac tttcggcaag cccaaacgag tgattctgga
    12961 tccagaccac aaagtactgc ggctcgatga caaaatccgg gtcgccgtgg cgatcaagaa
    13021 gggcgagcaa caggcggagc tcgggtatta caacgaggcg cttcaggagt accaaaaagc
    13081 cctcgacgtg aatcgaaaca gctccctggc gcattaccgc atcgcagaag tatttttctt
    13141 gcagcgcaac tatcaatccg cggccaatga attccgggag gccatcaacg gggacggcga
    13201 gcccaaatgg accgaggttt ggtcccatat caatctcggc aagatctttg atatcaccga
    13261 tcagcgggat cgggccgtaa acgaatacca gcaggcaatc cgcaccaaag acaacacgca
    13321 gaacgcgctg gacgaggcga acaagtattt gcaatcgccg taccggcggc cgtcgcgcag
    13381 cgaagtctga gaccgcggcc tcagccctct tcttcccggt agccggccag cgaccattcc
    13441 tgtgtcactt catcgaagcg cagcagatac agattcccat catcggctct cacgcggaag
    13501 tagcgatacc cggggccata ccagcgatcg atgatctcca cgaccgagta cctatgctcc
    13561 ccaagtagaa accttaaggg ggattcgtcc gccttatagc cgctatagga ttccaccctg
    13621 actcgcatgg ttaattctcc cgggccagcg gttttggatg cattttttcg aacccgttcc
    13681 gcttgacaat caggaaagga tggataaaat aagggcgatc tgtatgttcg tctcgcatgg
    13741 atgacgcaac gacctcttca taacatccgc acttaactgg aaaactgagt ccatcaatat
    13801 ggagccgtac tgcgagcact cgcgcaccaa aatcattgcc cgagatggca atgtggacta
    13861 cgtcgaatgc ctcgactgta aaaaagtttt cgaggcagag gacctcgagc cggtagccac
    13921 ggaacaagag gaagaggaag cagaagcctg aaggcggaga caaggccctt cctgttgctc
    13981 cctcgtgcat tggcggctcg ccgggggagg aggtccgcct tggccgcttc gcgagcggtt
    14041 ttgcgatctc gcgatctccg ggcggcttgg gaaactctgg ttccggcaag gcgggcggcg
    14101 ccgcgct
//
LOCUS       DASREK010000125         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1053968, whole genome shotgun sequence.
ACCESSION   DASREK010000125 DASREK010000000
VERSION     DASREK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053968"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VFA54_06255"
     CDS             complement(<1..981)
                     /locus_tag="VFA54_06255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZS50440.1"
                     /translation="MTSSRFRQIERLYHAVRERPTGERVSFLDEACAGDEDLRREIES
                     LLAQDISATNPLDRLGGALADAAGIEDTSAPVFAVGSEVGCYRIEGPIGEGGMGVVYK
                     ALDTRLNRPVAVKVLSSRLADAAARRRFQREAQTASSLNHPHILTVYDVGERDGEQYL
                     VTEYIDGGTLREWAGAGTRTWREIVELLVGVADGLASAHEANILHRDIKPANILVMKS
                     GYAKLADFGLAKLAGASPQPADATKTATVQTRPGMVVGTIAYMCPEQAQGRATDARSD
                     IFSFGVVLYELLAGRRPFDGRSDLETLHKVIETAPEPLGDNVPTALRAIVE"
     gene            complement(1212..1568)
                     /locus_tag="VFA54_06260"
     CDS             complement(1212..1568)
                     /locus_tag="VFA54_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZS50441.1"
                     /translation="MPAAVPRSAEITKLLKPWSGGDDAALESVVEQVCAELPVIARRY
                     MKNERQGNTLQATAFVNEVDLHLVDVTHVDWQERAQFFAMAAQMMPHISVDKPRARAS
                     DKRGGRVARLNIEKTP"
     gene            complement(2014..>2376)
                     /locus_tag="VFA54_06265"
     CDS             complement(2014..>2376)
                     /locus_tag="VFA54_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding acetyl-CoA synthetase"
                     /protein_id="HZS50442.1"
                     /translation="IESKLLTHPAVEACCLMGAGHPSPFAIVLLSMEAREKCTDADGQ
                     RQLAESLHLQMQNVNADLDPHERVAFLAVVDGPWTIGNGLITPTLKIKRTALEGRYQA
                     LFEEWQRQELPVVWEKLS"
BASE COUNT          428 a          797 c          691 g          460 t
ORIGIN      
        1 ctccacgata gcgcgcagcg ctgtgggcac attgtcgccg agaggttccg gcgcggtctc
       61 gattaccttg tgcagggtct ccagatcgct ccgcccgtca aacggccggc gccccgcgag
      121 caactcgtag agcactaccc caaaggagaa gatatcgctg cgggcgtcgg tcgctcttcc
      181 ctgggcctgc tccggacaca tgtacgcgat ggttcccacc accatgcccg gccttgtctg
      241 cacggtggcc gttttcgtcg catcagcggg ttgcggcgag gcgcccgcca gtttcgcaag
      301 cccgaaatcc gccagctttg cgtagccgct cttcatgacg aggatattcg ccggcttgat
      361 gtcccgatgc aggatattgg cctcgtgcgc ggacgccaaa ccatcggcca ctcctaccag
      421 aagttccacg atctcccgcc acgtgcgcgt gccggccccg gcccattcgc gcaacgtacc
      481 gccgtcgatg tactcggtaa ccaggtactg ctccccgtcg cgctcgccga cgtcatacac
      541 cgtgaggatg tgaggatgat tgagcgaaga cgccgtttgc gcttcccgct gaaaacggcg
      601 gcgtgccgcg gcgtcggcaa gccggctgga aagcaccttc accgccacgg gccggttcag
      661 ccgcgtgtcg agagccttgt agacgactcc catcccgcct tcgccgattg gcccctcaat
      721 cctgtaacag ccgacttccg accctacggc gaacaccggc gcgctggtat cttcgatacc
      781 ggccgcgtcc gccagcgctc cgccaagccg atcgagtggg ttcgtcgcgg agatatcctg
      841 cgccagcaaa gactcgattt cgcggcgcag gtcctcgtcc ccggcgcagg cctcgtcgag
      901 aaagctcaca cgctcgcccg tcggccgctc ccgcacggcg tgatagagcc gctcgatctg
      961 tcgaaagcgt gaagaagtca taagaatctc gcctatggtt tactgctgca actcctcatc
     1021 aaccatgcct tgccaagatc ccagtcgacc gcacggtccg caacgaattt tcagtaccga
     1081 aacgatctcc tcttccgtca ggccgccgga gtatctcaat tcgaccacct tcgtccggcg
     1141 cggctcgaca cgtgaaaatt cgtctaaggc ctcgtccaga gcgacgatgg agggcccggg
     1201 aaccggagcg atcacggcgt tttctcgata ttcagccggg caacccgccc gccccgcttg
     1261 tccgacgccc gggctcgagg cttatctacg gagatgtgcg gcatcatctg cgcggccatg
     1321 gcaaagaact gcgctcgctc ctgccaatcg acgtgggtca cgtcgaccag gtggagatcg
     1381 acctcattta cgaacgccgt ggcttgcagg gtattcccct gtcgttcgtt cttcatatag
     1441 cggcgagcga taacgggtaa ttccgcgcag acctgctcga ccacgctctc cagcgcggca
     1501 tcatcgccgc cgctccaggg tttgagtaac ttcgtgattt ccgcagaccg ggggaccgcc
     1561 gccggcattc tcactcacgc gtaccaaaaa atagcacagt cgtcccggtt tcgtcgttgg
     1621 ttccatgcac cggggcacag ggagcaggcg gtaacccctg tatctggtgc cggcaaactc
     1681 gatcgactcg gccggggaga acgtgggtgt ggctctgacg gctaatggcg aggttcacgg
     1741 tttcattctg atcccccggt gaaggaagcg ccggcaggct gccctgggcg gatcgcccag
     1801 ccggaacctg gcccggatca ggaacggtaa ttctcgtcag aaggcggccg atgcactcct
     1861 gatttagttt agcggctgtg aaggcggacc cctgtaccgc agcctgccgc cgtcggcttg
     1921 cggaagcgtg caatatgctc gaaatcggtc aagcggcccc ccgcgtgaac cacgggttcc
     1981 gtaacactga gaacgttatt atccagtcga aggctacgag agcttctccc agacgacggg
     2041 caactcctgg cgctgccatt cttcaaataa cgcctggtag cggccctcca gcgccgttcg
     2101 cttgatcttc agcgtcggcg ttatcaggcc gttgccgatc gtccacggcc cgtccacgac
     2161 cgccagaaac gccacgcgct cgtgcgggtc cagatccgcg ttgacgtttt gcatctgcaa
     2221 gtggagggat tcggccaact gtctctggcc gtcggcatcg gtgcactttt cacgggcttc
     2281 catcgacagc agcacaattg cgaacgggct cggatggccc gcccccatca ggcagcaggc
     2341 ctcgaccgcg ggatgtgtca acagtttgct ctcgat
//
LOCUS       DASREK010000126         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_559682, whole genome shotgun sequence.
ACCESSION   DASREK010000126 DASREK010000000
VERSION     DASREK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559682"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VFA54_06270"
     CDS             complement(<1..911)
                     /locus_tag="VFA54_06270"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS50443.1"
                     /translation="MRKTEIDPLRLLLFLLAGIVPAAAPALAQIGNSELFGTVRDASG
                     LVVAGAEVEVRDAGTGRTVKAATNDGGAFYFFALEPGEYAVAVSKAGFAPLRRSGIHL
                     RVADRVSLDLQLAIGDLSQTVDVSTSAPFLQTTTGAESFIADNIKVTTLPLDGRNFVP
                     LVALAPGVALPPGSTASPSLLPRINGSRPRTSEYIYDGISVLQPEPGQVAYFPVIDAI
                     EEFRVQLNSYSAEYGRSNGGVIQVETKSGTNDFHGTLFEFFRNEGLNARNFFAPFSPK
                     PPVKRNQYGLALGGPVQKNRTFFFADW"
     gene            1003..2115
                     /locus_tag="VFA54_06275"
     CDS             1003..2115
                     /locus_tag="VFA54_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZS50444.1"
                     /translation="MEVRNRVGEIRRKRGVAAADLAAQVGVSRQTIYAIEAGGYTPNT
                     VVALRLAEALGVRVEELFSLAREGAAAKMQTVDLLGAETLAPGQPLRLCQIGRRKVGV
                     FSSPAPFELPPADAIAAGPAGPQMALAEPLCDPDVDPAKRLLVAGCDPGMGVLARRLM
                     CEASVELITAGCSSLQALAWLREGKVHVAGLHLRDEASGELNVPAVKSVFPKGGYKMV
                     TFAVWEEGLIVRKGNPKKIRSISDLGRPDVAIVNREQGAGSRFLLDRELKKAGIAGAA
                     VRGYDRLTRGHIDAAWHVYAGQADCCLATTAAARVFGLDFIPLGSNRYDLVIPRRFLD
                     LRAVDAMLDRMTGRGFRLELEALGGYETSQTGKIVL"
     gene            complement(2125..2478)
                     /locus_tag="VFA54_06280"
     CDS             complement(2125..2478)
                     /locus_tag="VFA54_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HZS50445.1"
                     /translation="MVETEPVVKMHPKSEGIKTPRKKKPKEIAVITECCTGCAGSPAC
                     IPYCPVEECMYWVPDEEHPPFGHVEVDPQLCIGCKKCTSKGPDDTFLDGCPWDAIEMV
                     DTAEWEAAHGIKMAY"
     gene            complement(2722..3330)
                     /locus_tag="VFA54_06285"
     CDS             complement(2722..3330)
                     /locus_tag="VFA54_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZS50446.1"
                     /translation="MHLLPTPDEIIRILKDTGALRRGHFELPNGKHTDQFLQLPLALR
                     YYQHCRTLSVALSRLLRANLEISALVPELSVVTPASGGLPVAFGIAEALRTRQVYWAE
                     NEGGRLRFRQYMEDHQGEKVILVDDTLRTGQKLHELKALLESAGATIMALAVVVDQPH
                     GRRLQFDHIPLYSLVTLETEVYADAGCCAMCRSGQPLERVRT"
     gene            complement(3384..4559)
                     /locus_tag="VFA54_06290"
     CDS             complement(3384..4559)
                     /locus_tag="VFA54_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZS50447.1"
                     /translation="MNIDEIQAALCAEKLDGWLFFDHHRRDPIAYRVLGFEPRQHVTR
                     RWYYLIPAEGEPRKLVHRIESGMLDALPGARRVYSGWQEMRAELFGMLSGLRRIAMQY
                     SPDCMIPYVSLVDAGTVELVRGLGVEVASSSALVQRFEACWTAEQLEMHLEAGRRVDK
                     IRAEAFEEIGRRVRSAGLVQELEIRDFILSRFRDHNLIAEDGPIVGVNANSGNPHYEP
                     GPGRTSPIRSGDFVLLDMWAKLGRPGAVYYDITWTGYLGQEPPARISGIFEIVSSARD
                     RAIAFIQSAVRERRTICGYQVDDVARAYITEQGYGERFIHRTGHSIGEEVHGNGANMD
                     NLETHDDRPLAPGTCFSIEPGIYFPDFGVRSEVNVYIGPSEAQVTGEIQRAIVMIRV"
     gene            complement(4700..>5273)
                     /locus_tag="VFA54_06295"
     CDS             complement(4700..>5273)
                     /locus_tag="VFA54_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HZS50448.1"
                     /translation="GGLLPASWERLLPAFASHLADFVFPASWYQFVQPVFVVALAPVF
                     AWLWLALHRKGAEPSSPAKFSFGLLWAGAAFAILVPAALAASDGRQVGPWWLVGTYFL
                     QTLGELSLSPVGLSAMTKLAPERAAGFMMGIWFLSTSIGNWLAGKAASLYSSLPLPTL
                     FGSVAGFAIAAGVILALLAKRTKRLMGDVN"
BASE COUNT         1080 a         1599 c         1654 g          940 t
ORIGIN      
        1 tgccagtccg cgaagaaaaa cgttcggttc ttctgaaccg gccccccgag agctaagccg
       61 tactgattcc gcttaacggg cggcttggga ctgaaaggcg cgaagaaatt gcgcgcattc
      121 aggccctcgt tgcggaaaaa ctcaaacagc gtgccatgaa aatcgttggt tccggacttt
      181 gtctccactt gaatcacgcc gccattggaa cggccgtatt ccgccgagta gctattgagt
      241 tggacgcgaa attcctcgat cgcgtcgatc accggaaaat aggcaacctg gccgggctcg
      301 ggctggagaa cgctgatgcc gtcgtagatg tactcgctcg tcctcggccg gctcccatta
      361 atgcgcggga gcagcgaggg ggaagctgta gagcccgggg gaagcgccac gcccggagcg
      421 agcgccacca gcggcacgaa gtttctgcca tcgagcggca gggttgtcac cttgatattg
      481 tcggcgatga agctctcggc gccggtggtg gtctggagga atggcgcgga ggtggatacg
      541 tctacggtct gcgacagatc gccgatggca agttgcagat cgagagacac ccgatccgca
      601 acacgcaagt gaattccgga ccggcgcagg ggcgcgaacc cggctttgga aacggccacc
      661 gcgtactcgc cgggctccag ggcgaagaaa taaaatgcgc cgccatcgtt ggtcgcggct
      721 ttcaccgtgc ggccggttcc cgcatcacgc acctccacct ccgcaccggc gacgaccagt
      781 cccgaagcat cgcgaaccgt cccgaaaagc tcggagttcc cgatctgggc gagcgcgggc
      841 gcggcggctg gaacgattcc cgccaataga aacagcagaa ggcgcaaagg gtcgatttcg
      901 gtttttctca taaagacgac attgtaactt atacaagact tgtcatgcaa aggaaaaaat
      961 tgtagcgtat aagggacaag agcgcccccg agggcggagg ctatggaagt cagaaaccgg
     1021 gtcggagaaa tccggcggaa gcgcggcgta gcagcggcgg atctggcggc ccaggttggg
     1081 gtcagccggc agacgattta cgccatcgaa gccggcgggt acacgccgaa cacggtagtc
     1141 gccctccggc tggctgaggc gctgggggtg cgggtagaag agctgttctc gctggcacgg
     1201 gaaggcgcgg cggcgaagat gcagactgtc gatctgctgg gcgcggaaac gctcgcgccg
     1261 ggtcagcccc tccgcttgtg ccaaatcggc cggcgcaagg tgggcgtttt ctcatcgcca
     1321 gcgcctttcg aactgccccc cgccgatgcg atagccgccg ggccggccgg accgcaaatg
     1381 gctttggccg agccgctgtg cgatcccgac gtggatccgg caaaacgcct gctcgtggcc
     1441 ggatgcgatc cgggcatggg agtgctggcg cggcgcctga tgtgcgaggc ttctgtggag
     1501 ctgatcaccg cgggctgctc cagcctccag gcgctggcat ggctccgcga ggggaaggtt
     1561 cacgtcgccg gcttgcacct tcgcgatgaa gcaagcggag aattgaatgt cccggcggtg
     1621 aagagcgttt ttccgaaggg cggttataag atggtcactt tcgccgtctg ggaggaaggc
     1681 ttgatcgtcc gcaaaggcaa tcctaaaaag atccgcagca tatccgacct gggccgcccg
     1741 gacgtggcca tcgtcaatcg cgagcaagga gccgggagcc ggtttctgtt ggaccgtgaa
     1801 ttaaagaagg cgggcatcgc aggggcggcc gtgcgcggat atgaccgctt aacccgtggc
     1861 cacattgacg cggcctggca cgtctacgcg gggcaggccg attgctgtct ggcgacgacg
     1921 gcagccgcgc gtgtcttcgg gctggatttc atcccgctgg gcagcaaccg ttacgatctg
     1981 gtgattcccc gccgttttct ggatctgcgg gcggtcgacg ccatgctgga tcgaatgacg
     2041 ggccgcggat tccgcctcga attagaagcg ctcggaggct atgagaccag ccagacaggt
     2101 aagattgtct tgtgaaaaag gctttcagta cgccatcttg atgccgtgag cggcttccca
     2161 ctcggcggta tccaccattt cgatcgcgtc ccacgggcag ccatccagaa acgtgtcatc
     2221 aggacccttg ctcgtgcact tcttgcagcc gatacacaat tgcgggtcca cttccacgtg
     2281 gccgaaagga gggtgctctt cgtcgggcac ccaatacatg cactcttcca ccgggcaata
     2341 gggaatgcag gcgggcgagc cggcacagcc ggtgcagcac tcggtaataa cggcaatttc
     2401 cttgggcttt ttctttcgag gcgtcttgat gccctcggac tttgggtgca ttttgacgac
     2461 gggttcggtt tctaccatgg ctccctctag accgtttccc attatatcga aaacggccgt
     2521 cgcggcttca gccccggaaa acgggctgct cctcgctgct ctccgatgag ctgctgagta
     2581 tagaaaccgg ctgccggacc gcgagaaaac agcgcgtcca cacgagcctt gcggaagcga
     2641 ctgtcgatca catcactttt ccttgtacaa cccgcctggc ggggccgcaa tggaatcgtt
     2701 cctttccgga agcgcggctg gtcaggtgcg gactctttcc agcggctggc cggaacggca
     2761 catcgcgcag cagccggcgt ccgcataaac ttccgtttcg agagtgacaa ggctgtaaag
     2821 cgggatatgg tcgaattgga gccggcgccc gtgcggctga tcgaccacga ccgccagggc
     2881 catgatcgtc gcgccggcgg attccagcag ggccttgagc tcgtgcagct tctggccggt
     2941 gcgtagagta tcgtccacca gtatgacttt ttcgccctga tggtcctcca tgtactggcg
     3001 gaagcgcagc cggccgcctt cattttctgc ccaataaacc tggcgggtgc gcaaggcctc
     3061 ggcgatgccg aaggcgacgg gcaagccgcc cgaggcggga gtgaccacgc tcaactccgg
     3121 aaccaaagcg gaaatctcca gattggcccg cagcagccgg ctgagcgcaa cgctgagcgt
     3181 ccggcagtgc tggtagtagc gcagggcaag cggcagttgc aggaactggt ccgtgtgctt
     3241 gccgttgggc agctcaaagt ggccgcgccg cagcgctccg gtgtccttta aaatgcgaat
     3301 aatttcgtcc ggcgtcggaa gcagatgcat gctgttaatt attccacaca ggcaattgag
     3361 cgtaaccggc ccaaatccag cggctacacg cgaatcatca cgatagcccg ctggatctct
     3421 cccgtcactt gcgcttcgct cggcccgata tagacattca cctccgagcg cacgccgaaa
     3481 tccggaaaat agatgcccgg ctcgatggag aagcacgttc cgggcgccag tggacggtcg
     3541 tcatgggtct ccagattgtc catattggcg ccattgccat gcacctcttc gccgatcgag
     3601 tgccccgtcc ggtggataaa gcgctcaccg tagccctgct ctgtgatgta cgcgcgggcg
     3661 acgtcgtcca cttgatagcc gcaaatggta cgccgttcgc gcaccgccga ttggataaac
     3721 gcgatcgcgc gatcccgggc ggagctcacg atctcgaata tgccgctaat ccgcgcggga
     3781 ggctcctggc caagatagcc ggtccaggtg atgtcgtaat agacggcgcc aggacggccg
     3841 agcttggccc acatatcgag caggacgaag tcgccgctcc ggatgggcga cgtccggccg
     3901 ggccccggct cgtaatgcgg gttgccgctg tttgcgttca cgccgacgat cgggccgtcc
     3961 tcggcaatca agttgtgatc gcggaagcgg ctgagaatga aatcgcggat ttccaattcc
     4021 tgaaccaagc ctgccgagcg gacccggcgg ccgatctcct caaacgcctc ggcgcggatt
     4081 ttgtccaccc tgcgcccggc ttccaaatgc atctcgagct gctcggccgt ccaacaggcc
     4141 tcaaaccgct ggaccaaggc ggaggagctt gccacttcca cgcccaagcc gcgcaccagc
     4201 tccaccgtgc ctgcatccac cagagataca taaggaatca tgcaatcagg tgaatactgc
     4261 atcgcgatac ggcgcaaacc agagagcatg ccgaagagct cggcgcgcat ctcctgccag
     4321 ccggagtaga cgcggcgggc gccgggtaag gcgtccagca tcccgctttc aatacgatga
     4381 accagcttcc gcggttcgcc ctcggcggga atcagatagt accagcggcg cgtgacgtgt
     4441 tgccgaggct cgaatcccag aacgcggtag gcgatcggat cgcggcgatg gtggtcgaag
     4501 aaaagccagc cgtccagctt ttcggcgcac agcgcggctt ggatctcatc aatattcata
     4561 ggggcggtct cggatgcggt gaaggttcta tgtaaaaatc gaaggcgcct ggcaaccggc
     4621 tcatacggcc gccttgaaag ccgtgcctca tcgaacagcc gagcggtccg ttcgctccgg
     4681 gctgagaacg ttcaagaatc taattgacat cacccatcag cctttttgtc cgtttcgcca
     4741 gcagcgccag aattacgccg gcggcgatgg caaagccggc cacggatccg aagagcgtag
     4801 gcagcggcag cgaactgtaa agcgatgcgg ctttgcccgc cagccagttg ccgatgctcg
     4861 tcgaaagaaa ccagattccc atcatgaacc cggcagcgcg ctcgggagca agtttcgtca
     4921 tggcgctgag gcccacgggg ctgagcgaaa gctcgcccaa agtttgcaga aaatacgtgc
     4981 ccaccagcca ccaagggcct acctgccggc catcgcttgc ggcaagcgcg gccgggacca
     5041 ggatcgcgaa tgccgcgccc gcccacagta atccaaagga aaacttggcg gggcttgaag
     5101 gctccgcgcc tttgcgatgg agcgccagcc acagccaggc aaacacgggc gccagcgcga
     5161 ccacgaagac cggctggacg aactgatacc agctcgcagg aaaaacgaaa tccgcgagat
     5221 gagaagcgaa ggccggcaaa agacgctccc aactggcagg caaaaggccg cca
//
LOCUS       DASREK010000127        11367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1715672, whole genome shotgun sequence.
ACCESSION   DASREK010000127 DASREK010000000
VERSION     DASREK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715672"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1417)
                     /locus_tag="VFA54_06300"
     CDS             complement(35..1417)
                     /locus_tag="VFA54_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS50449.1"
                     /translation="MDLLLTHGYFLYEDAKELHIMKPYPPLGILYISSHLRQKGFAVE
                     IYDTTFGSRQELFGILEKGPPSVIGIGGNLMTRPSVLEISAAAKRAGWTVVLGGPEPA
                     NYPREYLEAGADVIVEGEAEATMEELLPALRSRRCDALAHVDGIFFRSEDGALARTAP
                     RALIPDLDAQPWPDRERIDIGRYLAAWRKRHGAGSISVITSRGCPYHCRWCSHSTYGK
                     THRRRSPGNVADEVEWILRRYEPDMLWMADDVFTIHPGWILDYAAEMKRRALRIPFEC
                     ITRADRVSPRIAEALAELGCFRIWIGSESGSQRILDAMQRGVKVEQVQRAIALCRGQG
                     IQTGMFLMWGYEGEELADIEATVEHVKACAPDVFLTTVSYPIKGTPYYYEVEPRLVRL
                     APWAGSTDRDFRIAGRHSRRFYQFADQLLKSSVALSRLNGEQPAAPDLKAQVELARRG
                     LQESFEETEV"
     gene            complement(1402..2886)
                     /locus_tag="VFA54_06305"
     CDS             complement(1402..2886)
                     /locus_tag="VFA54_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS50450.1"
                     /translation="MADVLLTHSNHLYFDRKQTRKMQPYPPLQTLIAAAALREEGLET
                     ALFDATLNPPEEGFYAALDQHRPRLVAVCEDNFNYLTKMCLARNRELAFFMCRAAKAR
                     GLPVLVNGSDASDHAGEYLAQGADFILIGEVEKTLVETAAKLLRSPQAETEGRLPGRV
                     AEEIRGLAYLDSVSGQVRRTPPREVIADLDRIPPPAWDLIDASQYRKAWLSAHGYFSV
                     NMVSSRGCPYRCNWCAKPIYGQSYHFRSPSRVADEMLYLKTRFQPDRLWFADDIFALS
                     PRWTEEFAEAVETRGAQIPFQMQSRCDLMTRDTVAALRRAGCAEVWMGVESGSQRVLD
                     AMEKGLRVQHVYQARENLLRHGIRACYFLQFGYPGETWIDIQSTIHLVRETKPDDIGV
                     SVAYPLPGTKFYGLVKAQLGSQKNWLDSDDLAMMFQGAYTSEFYKALRDALHLEVDIA
                     QKRKTGSSREQLCVLWRQVEQMQKTCSNPNATALPDPAPVWTCC"
     gene            complement(2888..4390)
                     /locus_tag="VFA54_06310"
     CDS             complement(2888..4390)
                     /locus_tag="VFA54_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS50451.1"
                     /translation="MRSKKVVFFFPAFSSQEATAPLAILAISTPLLRAGYQVRIIDST
                     ITPNFQKQVLKELENALCLAVSLVTGPMIRETVQIARAAKALYPGLPVILGGWHPSLL
                     PDQTLAAEFIDVVVRGQGEDALLEILERLEAGASLEGVAGVGYKQDGRLIFNPPRPLK
                     PIRDLPPKAYHLADFDAYERLCGRRWAMYISSLACPYSCSYCTNQGVYGRQWNALDAG
                     QAAQEMSDLVSRYRLSLLWVVDDNFLVDRERAVAIAEELLRRGVKFDWSIQASTNLVT
                     RLTTEELKLLRRAGLSQVSQGADSGSPKILHLMNKDFQKIETVYEAAHKLHEVGIRPS
                     FNMIFGYPGEGEKERRESIALIMDICRRFPGAEFWTNIFTPYPGSPVMERAFELGIDV
                     PKSLEGWADFFPRYTVLPWLKGRKHHRLQIMREYLRVAFHRVPIAADRRNRVTRAVHE
                     AISVPARWRLDHDFYVFPFELWLKNAAARVLQAPKPKVDARQLSAEPVTC"
     gene            complement(4585..5898)
                     /locus_tag="VFA54_06315"
     CDS             complement(4585..5898)
                     /locus_tag="VFA54_06315"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZS50452.1"
                     /translation="MTLTWKKAALPGPVQAALHALRLDETDPEMLCRLNEDQWRQALR
                     FTDPAQLTLVLQGRLAEAGVCKRLPPSIRLRFHRNWSDNTERLARTRTEYSAIAERLT
                     ASGVDFVALKGLTHSPHFTPDPRLRVQYDIDLFCPPQDVYRARDALRKMGYETVTALE
                     GLPTDHLPAMVRKTGWRWRGNYFDPELPLSVDLHFRFWDSETERLPAPGVEAFWERRA
                     GHDLDLADRLGYAALHALRHLLRGSLRLHQIYEIAYFIQKHSGDADFWRRWNLAHEPG
                     MRTLESVVFRLAGCYFGSPRLDAFAPLPAPVERWLEQYAWSPVEAGFRPNKDELYLHV
                     SLLANRADRWFVIRRRMFPITWPGPVEGVHAPDSQKTRASRIRDRIQWAGFVASRLMF
                     HARAMGPAIWGLARCMKCSRAGHASQPLPPREHTTVITDPRHSGD"
     gene            complement(5910..6890)
                     /locus_tag="VFA54_06320"
     CDS             complement(5910..6890)
                     /locus_tag="VFA54_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50453.1"
                     /translation="MDSKRYEQWQTPDPLGYRFEAPYTESYSPLGFPLILRTNSQEVL
                     AAGRRSWEESEAAFETKPIELRVLVLGDEAEPPRALPEGPVYRAQGHLLALVLGTENF
                     AVCDLDRGFGFARLTPGVVKNHLFTAFHFLDCMAYLCLSHQHLTPIHAACVVRNGRGI
                     LLAGGPGAGKSSLAWACARAGMTFVSDDATWLLRKEPVLIGRPQRMRFRPDAVQLFPE
                     LARFPRWDTVIGKHSFEIRTADVPGLLTVRCCRPGPVVFLDRQPSGEAQLDRVGPEEA
                     GRRLAAATTLYEFQVWKQQEKSREILLQQGALRLRYSTLASAVQQISKIE"
     gene            7091..7732
                     /locus_tag="VFA54_06325"
     CDS             7091..7732
                     /locus_tag="VFA54_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS50454.1"
                     /translation="MADIVAGFALPDRDLELIEKLREGSDEAFASLLSLYERPVYNFV
                     FRLLGDAADAPDVTQEVFLKVFRGIGDFRGQCSLKTWIYRIALHEASNFRRWFSRHRC
                     GELSLDEKEEGRNGFADTLRDGQESQYEQVLRHERIRVIERALSQIHEPYRVAVVLRD
                     IEGLSYEEIAEILQVSLGTVKSRILRGREALKHALAALDPKRSLTACPLEAVE"
     gene            7736..8389
                     /locus_tag="VFA54_06330"
     CDS             7736..8389
                     /locus_tag="VFA54_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50455.1"
                     /translation="MNCVFAREKLTAFLCEELDASQSAGLRRHLEKCSGCHEEYEYQA
                     RLISPLRELPRIEPPANLAMQIRVRLSYRDQPGFWERWQVYLSNFLRPVALPAAGGLL
                     TALMLFAILMPAVAVSRAVALTNDVPTVLSTEPRFKQASFLPLNEDILVEAWIDEQGR
                     VSYFQVLNPVQGSVPEKALQAQLNNVMLTTFFEPATRFGQPTASKVLLALRRINIRG"
     gene            8432..9331
                     /gene="rsmA"
                     /locus_tag="VFA54_06335"
     CDS             8432..9331
                     /gene="rsmA"
                     /locus_tag="VFA54_06335"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZS50456.1"
                     /translation="MPASRRAPLGQNFLASPSYLARIAGEIRSAVTGGQRPDSAQAAP
                     PKSGAASLVVGPAPAPSADLIVEIGAGSGALTKYLLELATPLVAVEIDAKLAESLRAA
                     LPSPLLDVVNADILKVDLRRLLEERGARRAVVAGNLPYYITSPVLHHIFKAAGSVSDA
                     VLLVQKEVAARIAARAGSREYGYLSVLCRVYSRPELCFTVPPGAFRPAPKVTSALVRL
                     HFEPHWAAWGVDDPARFLDFVKLCFHQKRKTLLNNLAAKFGDRLRSGALARLPELRLR
                     AEQLEPERLAALWLLLERAEAPV"
     gene            9556..10518
                     /locus_tag="VFA54_06340"
     CDS             9556..10518
                     /locus_tag="VFA54_06340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZS50457.1"
                     /translation="MRILLAEDDPVSRKLVESFLAKWSHEVLFAADGNEAWALLQEPD
                     GPRLAILDWMMPGMDGVHICREIRKQTGRPYVYLMLLTSKSEKQELLAGLEAGADDYL
                     VKPFDPEELRYRLRAGKRILDLQQDLLSMREAMQHPESYDPLTGLANRESILGLLRQK
                     LVQERGFRPLGVILANVDCFRQAVHAYGLPAGDMVLREVARRLRSVVDSRGVIGRTRT
                     GEFLIIMSGCDPAQLMRQAEELRTAVSASPIDLLGAAINVSISAGVATAAEASDVSAL
                     MIAADAAVRRAKAKGRNRIEPAALADPGALRGITGPNPPLHPTS"
     gene            complement(10587..>11367)
                     /locus_tag="VFA54_06345"
     CDS             complement(10587..>11367)
                     /locus_tag="VFA54_06345"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HZS50458.1"
                     /translation="YWGRAASLIHTSIDGRSDARIPENVRIYMFAGGQHGPAAFPPTR
                     TIGQQLNNPNDYRWAMRALLVAMDRWVTAGAAPPPSAYPQIAKDSLVTPQAVQFPRIP
                     GVNFPTIIHKAYRVDYGPQWKAGIIGFEPPKVGAPFPILVSQVDLDGNEIGGLKMPEV
                     AVPLATYTGWNLFDGKAGPSDELASMQGSYIPFPRTRAERERNHDPRRSIEERYYDRS
                     EYMGEVSDAALRLIEQGYVLDRDLPAILEQANVRWKRATGQ"
BASE COUNT         2210 a         3487 c         3506 g         2164 t
ORIGIN      
        1 cggggggcca atcggtcaaa cgcagccgcg gcgctcatac ctcggtttcc tcgaaacttt
       61 cttgcagccc gcgccgcgcc agctccacct gcgctttcag atccggagcc gccggctgct
      121 cgccgttcag ccggctcaag gcaaccgagc ttttcagcag ttggtcggcg aactgataaa
      181 accgccgcga atggcggccg gcgatcctga agtcgcggtc cgtggaaccg gcccagggcg
      241 caaggcgcac caggcgcggc tctacctcgt aataataggg agttcccttg atcgggtagg
      301 agacggtcgt cagaaagaca tcgggagcac aagcctttac gtgttccacg gtcgcttcga
      361 tgtccgcgag ctcctcgcct tcgtatcccc acatgaggaa catgccggtc tgaatgccct
      421 gcccccggca cagagctatg gcgcgctgaa cttgctccac cttgacgccg cgctgcatgg
      481 cgtccagaat gcgctgcgaa ccgctttccg agccgatcca tatgcggaag cagccgagct
      541 cggcgagtgc ttcggcgatg cgcgggctca cgcggtcggc gcgcgtaatg cactcaaagg
      601 gaatccgcag ggcgcggcgt ttcatctccg cggcgtagtc cagtatccag ccgggatgaa
      661 tggtgaacac gtcgtccgcc atccacagca tgtccggctc gtagcggcgc aagatccatt
      721 cgacctcgtc cgccacgttg cccggcgagc gccggcgatg agtttttccg taggtcgagt
      781 gactgcacca gcggcagtga tacgggcagc cccgggatgt aattactgag atcgatccgg
      841 ccccatgccg tttccgccat gcggccagat accggccgat atcaatgcgc tcgcgatccg
      901 gccagggctg cgcgtcgaga tcggggatca gggcgcgcgg cgccgtgcgg gcgagcgctc
      961 cgtcctcgct ccggaaaaaa atgccgtcca cgtgggcgag ggcgtcgcag cgccgcgacc
     1021 gcagcgccgg aagcagttcc tccatggtgg cttccgcttc accctctacg atcacgtcgg
     1081 cccctgcttc gagatattcg cgcgggtaat tcgccggctc cggaccgccc agcaccaccg
     1141 tccagccggc ccgcttggcg gccgccgaaa tttctaggac gctgggccgc gtcatgaggt
     1201 tgcccccgat gccgatcacc gacggcgggc ctttctccaa aattccgaac agctcctgcc
     1261 gagagccgaa cgtcgtgtcg taaatttcga cggcaaagcc cttctgccgc agatgcgaag
     1321 agatataaag aatgccgagc ggcgggtagg gcttcatgat atgaagctct ttcgcgtctt
     1381 cgtagagaaa atagccgtgg gtcagcagca ggtccataca ggcgccggat ccggcagtgc
     1441 cgtagcgttc gggttggagc aagtcttttg catctgctcc acctgccgcc acaacacgca
     1501 gagctgctcc cggctcgaac cggttttccg cttctgcgcg atatccactt cgaggtgcag
     1561 cgcgtcgcgg agcgccttgt agaactcgct cgtgtaggct ccttgaaaca tcatcgcgag
     1621 atcgtcgctg tcgagccagt ttttttggga accgagctgc gcttttacca ggccgtaaaa
     1681 cttcgttccc ggcagagggt aggccacgga aacgccgata tcgtcgggtt tcgtttcccg
     1741 aactaggtga atggtgctct ggatgtcaat ccaggtttcg ccggggtatc caaactgcaa
     1801 aaaataacaa gcgcggattc catgccggag caggttttcg cgagcttggt aaacgtgctg
     1861 tacgcggagg cccttctcca tggcgtcgag gaccctctgc gatcctgatt ccacgcccat
     1921 ccaaacctcg gcgcagcccg cccgccgcag cgccgccact gtgtcgcgcg tcatgagatc
     1981 gcatcgcgac tgcatctgga acggaatctg cgcgccgcgg gtctccacgg cctccgcaaa
     2041 ttcttccgtc cagcgcgggg aaagcgcaaa tatatcgtcg gcaaaccaca agcgatcggg
     2101 ctggaacctg gttttcaggt acagcatctc gtcagcgact cgagaaggag accggaagtg
     2161 atagctctgc ccgtaaatcg gcttggcgca ccagttgcac cggtaagggc agccccggct
     2221 cgagaccatg tttacggaaa aatagccgtg ggcggacagc caggccttgc ggtattggga
     2281 tgcatcaatc aggtcccagg ccggcggggg aatccggtcc agatcggcga tcacttctcg
     2341 cggaggagtc cggcgcactt ggcccgagac agagtccagg tacgccaggc ctctgatctc
     2401 ttcggcaacg cggcccggca gccggccttc tgtttccgcc tgcggggagc gaagcagctt
     2461 ggccgcggtc tcgaccagcg ttttctcgac ctcgccgatg aggatgaaat cggctccctg
     2521 ggccagatat tcgccggcat gatcggaagc gtccgatcca ttgaccagca ccggcaggcc
     2581 ccgcgccttg gccgcacggc acataaaaaa ggccaactcg cggttccggg cgagacacat
     2641 ttttgtcaaa taattgaaat tgtcttcgca cacggccacc aaacggggcc ggtgctggtc
     2701 gagcgcggcg taaaatcctt cttctggagg attcaacgtc gcatcgaaaa gcgccgtctc
     2761 aaggccttct tcgcggagcg ccgccgccgc tatgagcgtc tgtagcggcg gatacggctg
     2821 cattttacga gtctgtttcc ggtcgaagta gagatgattg gagtgggtga gcagtacgtc
     2881 ggccatctca gcacgtcacc ggctcggcgg agagctgccg ggcgtccacc ttgggtttgg
     2941 gagcttgcaa tacgcgggcg gcggcgtttt tcagccaaag ttcgaacggg aacacgtaaa
     3001 aatcatgatc cagccgccag cgggcgggaa cgctaatcgc ctcgtgcacg gcgcgcgtga
     3061 cgcggttccg ccggtctgcg gcgatcggca cgcggtgaaa cgcgacgcgc agatattcac
     3121 gcatgatttg caagcggtgg tgcttgcgtc cctttaacca gggcagcacc gtgtagcgcg
     3181 ggaaaaagtc ggcccagcct tcaagcgatt ttggaacgtc gatgccaagc tcaaaagccc
     3241 gctccatcac gggagatcct ggataaggcg taaagatatt ggtccagaat tcggcgccgg
     3301 gaaaccggcg gcagatgtcc ataatcaagg caatgctttc gcgccgttcc ttctctccct
     3361 cgcccgggta tccgaaaatc atgttgaagg aagggcggat gccaacttcg tgaagtttgt
     3421 gggcggcctc atagaccgtc tcaatcttct gaaaatcttt gttcatcaaa tgcagaatct
     3481 tcggcgagcc ggagtccgcg ccttgcgaaa cctgcgaaag gccggcgcgc cgcagcagct
     3541 tgagctcctc ggtggtcaag cgggtcacca ggttggtaga ggcctggatg ctccaatcga
     3601 acttcacccc gcgccgcagc agctcctcgg cgatcgccac agctcgttcc cggtccacga
     3661 gaaagttatc gtccaccacc cacagcaggc tcaggcggta ccgggagacc agatccgaca
     3721 tttcctgggc cgcctgcccg gcatcgagcg cattccactg ccggccatag acgccctggt
     3781 tggtgcagta ggaacaacta tagggacaag ccaggctgga aatgtacatt gcccagcggc
     3841 gcccgcagag gcgctcataa gcatcgaaat cggccagatg gtatgccttc ggcggcaaat
     3901 cccggattgg tttcaaaggc cgcggcggat tgaatatgag ccggccatcc tgtttgtaac
     3961 ctacgcctgc gacgccctcg agcgatgctc ccgcttccag ccgctccaga atctccagca
     4021 gcgcgtcctc cccctggcct cgcacgacta cgtcgatgaa ttctgcggcc aaagtctgat
     4081 ccggcaacag cgaggggtgc cagccgccga ggatcacggg aagtccggga tacaaggcct
     4141 ttgcggcccg cgcaatttgc accgtctcgc gaatcatagg tcctgtcacg agcgatactg
     4201 ccaggcacag agcattttcg agttctttaa gaacttgctt ctgaaaattc ggtgtgattg
     4261 tggaatcgat aattcgaacc tggtagccgg ctcgcagcag cggagtcgaa atcgccagta
     4321 tggccagcgg cgccgtcgct tcctggctgg aaaaggcggg aaagaaaaaa acgacctttt
     4381 tggaccgcat cgggcgattc gcgcgggcaa cagccgcaca cgacgaatgt tattctaacg
     4441 cccgccagca gggatcaaat gtaaaacctt ccgagccggc aggagctgcg gaaggaacga
     4501 tcgggtctga aatggactct gaggacgggt actgggaaac acttttcgtc gtcctgcgac
     4561 ggcgaaccgg gcgcaggatg cgtgtcagtc ccccgagtgg cgtggatccg tgatcacggt
     4621 ggtatgttct cgcggcggaa gcggttgcga agcgtgtcct gcgcgggagc atttcataca
     4681 tcgtgctaag ccccaaatgg ccggtcccat cgctcgcgcg tgaaacataa ggcgggaagc
     4741 cacgaatccg gcccattgga tacggtctcg gatccgcgag gcgcgagtct tttgactgtc
     4801 gggcgcgtgg accccctcaa cagggccggg ccaagttatc ggaaacatac ggcggcggat
     4861 cacaaaccac cggtcggcgc ggtttgccaa caggctcaca tgcaggtaca gctcatcctt
     4921 gtttggccgg aagccagctt ccaccggcga ccaggcatat tgctccagcc atctctccac
     4981 tggagccggc agaggcgcaa acgcgtccag gcgcggcgaa ccgaaatagc acccagccaa
     5041 ccggaaaaca accgattcaa gcgtccgcat tccgggctcg tgcgccaggt tccatcgccg
     5101 ccaaaaatcg gcgtccccgc tgtgtttctg aatgaaatag gcgatctcgt agatttggtg
     5161 caggcgcagg ctgccgcgca gcaggtgccg caaggcgtgg agcgcggcat atcccaggcg
     5221 atcggcgaga tcgagatcgt gccctgcgcg gcgctcccaa aacgcttcca cgcccggagc
     5281 gggcaatcgt tccgtctcag agtcccagaa gcggaaatgc agatccacgg agagaggcag
     5341 ttcgggatcg aagtagttgc cgcgccaccg ccagccggtt ttacgaacca tagccggcaa
     5401 gtggtccgtg ggaaggcctt ccagggcggt tacggtctca tagcccatct tccgcaacgc
     5461 gtcccgggcg cggtagacat cttgaggcgg gcagaacagg tcaatgtcat attgcacacg
     5521 gagccgggga tcgggtgtaa agtgcggcga atgcgtaagc cccttgagag ccacgaaatc
     5581 cacgcccgag gctgtcagcc gttccgctat ggccgagtac tcggtccggg tgcgcgcgag
     5641 ccgctcggtg ttatcgctcc agttgcggtg aaagcgaagg cgtatggaag ggggcaggcg
     5701 tttgcatacg ccggcctcgg caaggcgccc ctgaagcacg agcgtcaatt gcgcgggatc
     5761 ggtgaaacgc agcgcctgcc gccactggtc ctcgttcaag cggcaaagca tttccggatc
     5821 ggtttcatcc aggcgcaaag catggagggc tgcctgaacc ggccccggga gcgccgcttt
     5881 tttccacgtt aaagtcatgg cctgcgccgc tactcaattt tcgaaatctg ctggacggcg
     5941 ctggcgagag tgctgtagcg caaccgcagc gctccttgtt gcagcaagat ttctctcgat
     6001 ttctcttgtt gcttccatac ctgaaactcg tagagcgtag tcgccgccgc gagccgccgc
     6061 ccggcttcct cggggccgac tcgatcaagc tgggcttcac ccgagggctg gcgatccaaa
     6121 aaaacgaccg gccccgggcg gcagcaccgg accgtcaaca accccgggac atcggccgtg
     6181 cgaatttcga acgaatgctt ccctatcact gtgtcccatc gcggaaaccg cgccaattcc
     6241 gggaacaatt ggaccgcatc cgggcggaat cgcatccgtt gcggcctgcc gatcaggacc
     6301 ggctcctttc gcagcagcca ggtcgcgtcg tcggaaacaa aagtcatgcc ggcccgcgcg
     6361 caagcccacg cgagagaact tttgccggcg ccaggccctc cggccagcag aatgccacgt
     6421 ccgttgcgca ctacgcaagc agcatggatt ggcgtgaggt gctggtgcga gaggcacagg
     6481 taggccatgc agtccaaaaa gtgaaaggcg gtgaaaaggt gatttttcac tacgccgggc
     6541 gtgagccgcg cgaagccgaa gcctcgatca agatcgcaga ctgcgaagtt ctcggttccc
     6601 agcaccaggg ccaacagatg cccctgtgcc cgatagacgg gaccttctgg caaagcccgc
     6661 ggcggctccg cttcgtcccc gagcaccaga acgcgcagct caatcggctt cgtttcaaaa
     6721 gcggcctcgg attcctccca actccggcgg cctgcggcga gcacctcctg cgagttggtc
     6781 ctgagaatca gagggaagcc gagaggcgaa tacgattcgg tgtagggcgc ctcgaagcgg
     6841 tagcccagag ggtccggcgt ctgccattgc tcgtatcttt ttgaatccaa tcaagttccc
     6901 ccatttataa cggcgggatt gcccccggcc tgcccctacg atggtaacag aagccgggga
     6961 gcgggaactt tcaacccccg ccggtgtcta cctaagatgc agggctgaaa acgcgccttg
     7021 caaagcgttc ctgatcgttg gtaaggtgag gggtagacgg acataattct catttactgg
     7081 agaaacggca ttggcggata ttgtcgccgg tttcgcgctt cccgaccggg atctggaact
     7141 gatagaaaaa ttacgagagg ggtccgacga ggcgtttgca tcgttgcttt cgctgtatga
     7201 gaggccagtc tacaacttcg tttttcgctt gttgggcgac gcggcggatg ccccggacgt
     7261 aacccaggag gtgtttctga aagtgtttcg cggcatcggc gactttcgcg gccaatgcag
     7321 cctgaaaacc tggatttatc gcatcgcgct gcatgaggcc tcgaatttca ggcgctggtt
     7381 ctcacggcat cgctgcggcg aactatcgct ggatgaaaag gaggaaggcc gtaacggatt
     7441 cgccgatact ctccgagacg ggcaagagag ccagtacgag caggttttgc gccatgaacg
     7501 catccgcgtc atcgaacgcg ccctctcgca aattcacgag ccttaccgcg tcgcggttgt
     7561 gctgcgcgac atcgaaggcc tgagctatga ggagatcgcc gaaattctgc aagtatcatt
     7621 aggtacggtg aaatcccgca tactgagagg gcgcgaggcg ctgaagcacg cgctggcggc
     7681 gctggacccg aagcgcagcc tgacggcgtg ccctctggaa gcagtggaat aatgtatgaa
     7741 ctgtgtcttc gcgcgtgaga aactgacggc gttcctttgc gaagaattgg acgccagcca
     7801 gtctgctggg ctgcggcgcc atctggagaa atgctccggg tgccacgagg agtatgaata
     7861 tcaagcccgg ctgatctccc cgctccgcga attgccgagg atcgagccgc ccgccaatct
     7921 ggcgatgcag atccgggtcc ggctgtctta tcgcgatcag ccgggattct gggaacgctg
     7981 gcaggtctat ctctccaatt ttctgcgccc ggtggcgctc cccgcggcgg gtggtttgtt
     8041 gaccgccctg atgctcttcg ccattctcat gccggcagtg gcagtgagcc gggccgtggc
     8101 gcttaccaat gatgtgccca ccgtgctgag cacggaaccc cggttcaaac aggcttcgtt
     8161 ccttcctttg aacgaagata ttctggtcga agcctggatc gacgagcagg gccgcgtttc
     8221 ctactttcag gtgttgaatc cggtgcaggg aagcgttccg gaaaaagctc tgcaagcgca
     8281 actgaacaac gtgatgctga ctaccttttt cgagccggcc acgcgcttcg gccagcccac
     8341 tgccagcaag gttctccttg cgctccggcg aatcaatatt cgcggataac gccgcccttt
     8401 cgggcgctga ctgaacgcgc ccggactccc attgcccgcc tcgcgccgcg ccccgctcgg
     8461 acaaaacttc ctcgccagcc cctcctatct ggctcggatt gcaggggaga tacgtagtgc
     8521 cgtgacaggg ggccagcggc ctgattccgc ccaggcagca ccgccgaaga gcggcgcggc
     8581 gtcgctggtg gtagggcctg cccccgcgcc cagcgcggat ctgattgtcg aaatcggggc
     8641 cggctcggga gcgctgacca aatatctgct ggagctggct acgccgctag tggccgtcga
     8701 aatcgacgcc aagctggcgg aatcgctgcg cgccgctctc ccgagcccgc tgctcgatgt
     8761 cgtcaacgcc gacattctca aagtggactt gcgtcgattg ttggaagagc gcggcgcccg
     8821 ccgggccgtg gtggccggca atcttccgta ttacattacc tctcccgtcc ttcaccacat
     8881 cttcaaagcc gctgggagcg tgtccgacgc cgtattatta gtacaaaaag aggtggccgc
     8941 ccgtattgcc gcccgcgcgg ggtcccggga gtacggctat ttgagcgtct tgtgccgggt
     9001 ttatagccgg ccggagctgt gtttcaccgt cccgccgggg gccttccgcc ccgctcccaa
     9061 ggtaacttcg gcgctcgtgc gtcttcactt tgagccgcat tgggcggcgt ggggcgtaga
     9121 cgaccccgcg cggtttctcg atttcgtgaa actctgcttt catcagaagc gaaagacgct
     9181 gctcaacaat ctggccgcga aattcggcga ccggttgcgc tccggggctc tggcgcgcct
     9241 tccggaactg cgtttgcgag ccgaacagtt ggagcccgaa cggctggctg ctctctggct
     9301 cctgctggag cgcgccgaag ctccggtttg acaggcgcgg cgcagagggt cgagttcccg
     9361 gggccgcacg cgcctcgagt aggcaaaaag cctgctcaca gcagccagga gaaaaccgtg
     9421 agattgcgtt ttggggcgcg caacaagggc gcttgcgcga attgtgaact aggaaagcta
     9481 cctcagggcg ggggtactgg cgacctattg gctcgtgaga gtatgccgga ttagcataag
     9541 agcgggtcag cgccgatgag aatactgctc gccgaagacg atccggtcag ccgcaaatta
     9601 gtcgagtcat ttctggccaa atggagccac gaggtgcttt ttgccgctga tgggaatgaa
     9661 gcgtgggccc ttctacaaga accggatggg ccgcgcctgg cgatcctcga ttggatgatg
     9721 cccggaatgg acggcgtgca catctgccgg gagatccgta agcaaacggg cagaccttat
     9781 gtttacctca tgctgcttac gtcgaaatcg gagaagcagg agctgctcgc cggcctcgaa
     9841 gcgggcgcgg atgactatct ggtgaagccc ttcgatccgg aagagttgcg gtaccgtctt
     9901 cgggcaggca agcgcattct ggatcttcaa caggacctcc tttctatgcg ggaggcgatg
     9961 cagcacccgg aatcttatga tcccctgacc ggtttggcca accgggagtc cattctcggg
    10021 ctcctgcggc agaaacttgt tcaggaacgg ggattccggc cgttgggcgt catactcgcc
    10081 aacgtcgatt gcttccggca ggccgtccat gcgtatggcc tgcccgccgg ggatatggtg
    10141 ctccgcgagg tggcgaggag actgcgttct gtggtggact cgcgcggcgt catcggccgg
    10201 accaggaccg gcgagtttct cattattatg tcggggtgtg acccggctca actgatgaga
    10261 caagccgaag aattgcgaac tgccgtcagc gccagcccca tcgacctgct cggcgcggct
    10321 atcaatgttt cgataagcgc gggcgtggct accgccgcgg aagcttccga tgtttccgca
    10381 ctaatgattg cagccgatgc cgctgtgcgc cgcgccaaag cgaaaggccg aaaccgcatc
    10441 gagccggcgg ccctggccga cccgggcgcc ttgcgcggaa taaccggtcc gaacccgccg
    10501 ctgcatccaa cttcttagcg cagcgaatct tcgaaatcca cgggatccac tttgcccgcg
    10561 gaaaaccctg cgcccggcag cctcggtcac tgccctgtcg cgcgcttcca gcggacattg
    10621 gcctgttcga ggatcgcggg caaatcccga tcgagcacgt agccctgctc gatcagccgc
    10681 agcgccgcgt cggaaacttc tcccatatac tcgctgcggt cgtaatagcg ctcttcgatc
    10741 gaccggcgcg gatcgtggtt ccgctcgcgt tccgcgcgcg tgcgcgggaa cgggatatag
    10801 ctgccctgca tgctcgccag ttcgtcggaa ggaccggcct ttccgtcaaa caaattccag
    10861 ccggtgtaag tcgccagcgg caccgcgacc tcgggcattt ttaagccccc gatttcattg
    10921 ccgtccaggt ccacttgaga aaccaggatg gggaagggcg cgccgacttt cggcggctcg
    10981 aacccgataa tgccggcctt ccactgcggc ccgtaatcca cgcgatacgc cttgtggatg
    11041 atagtcggaa aattgacgcc cgggattctg ggaaattgca cggcttgcgg ggtcacgagc
    11101 gagtccttcg cgatctgcgg gtaggcgctg ggaggcggcg cggcgccggc ggtgacccag
    11161 cggtccatag ccaccagcag cgcgcgcata gcccagcggt aatcgttcgg attgttcaat
    11221 tgctggccga tggtgcgcgt cggcgggaac gcggccgggc catgctgccc gccggcaaac
    11281 atgtaaatcc ggacgttctc cggaattctc gcgtcggacc ggccgtcaat cgaggtgtgg
    11341 atcagcgacg ccgcgcgccc ccaatat
//
LOCUS       DASREK010000128         9171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_399280, whole genome shotgun sequence.
ACCESSION   DASREK010000128 DASREK010000000
VERSION     DASREK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9171
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399280"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="VFA54_06350"
     CDS             <1..1833
                     /locus_tag="VFA54_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50459.1"
                     /translation="QASVFRRNGALLSSFGPLLAAMHPAGDVPLRPVPRGGERSFPLL
                     RLAMLASDARGPALISALNFSETRPVDSDIQTADPRTGRPLVLRGLNLPPRQALLMPV
                     NFPLSQPEICGDCSSFAPDERLVWATAELLSVSFENGVLAMEFAAPAEGELTLELARE
                     PKGPLITGGHIRPFDWDEKTHRLHVRIPAGPAPEYRTRVGLAIDLPDSSVFLKTARRL
                     VIGSTAKVTAVFSPPALARRSRLLAPPGWRLKADPPGKDALPGEIDYSVVVPAGAVAG
                     DTVTLAVEADGKIVQAATLPLAPFCTVRIEPEEALHPRPDERFAIRPYLAALHLPGRR
                     AYRVLLRNNYDEIRNFEITASGEGLVFSPSHLEVSVGGGLERQITFYAAPAGGSGQNP
                     GTDDSPGRGNSGSERSRTGLYRGNLEIRQGSQKSNVPLALAVIAPGESLAYEFDMDRD
                     GSPELVLENRKLRAVFFPRQGGRSTEFLLKDEGINAFSIRGALDAGFPMEGRVLGPGR
                     IELHGGGLTRIISLGGEDSYFEVEETGRSGDWLVSAAPDLAWAGPKGHARFAIDVPNA
                     RIEADRKPFSINYRVQFADLPGPGSRSARFLFEWIEEHASQPGK"
     gene            1976..2407
                     /locus_tag="VFA54_06355"
     CDS             1976..2407
                     /locus_tag="VFA54_06355"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HZS50460.1"
                     /translation="MDKGEHEPEVKITDRRRFSREGEPLQPEEEDAAEEKTERAAEPA
                     APPSQPAVNHTPLPPPTFDVLVLSLRWQAELQLGMFSDDKEQEPMPDLEGARHSIDLL
                     GMLQEKTRGNLTLEEQRLLENSLTELRFRYVEAVERFAKKK"
     gene            2408..3199
                     /locus_tag="VFA54_06360"
     CDS             2408..3199
                     /locus_tag="VFA54_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS50461.1"
                     /translation="MPANGTVQITVLGSGTSVGVPTIGCDCRVCRSDDPRDQRLRPSI
                     LVSYNNRNVVIDTGPDFRMQALRAGFRTLDAALYTHAHADHILGLDDVRPFNYGHSEN
                     IPIYARPEVLTVIRRVFRYIFEETNHCGGIPRVEACPLDDSGVELFGLRFIPVPVLHG
                     ERLIVGFRFGRAAYLTDHSDIPEQSMPLLEGLDVLFLDALRHVPHPTHSTVERSLRWV
                     EQLRPARAYFTHICHELSHEETDRSLPPNVRLAYDGLRILVGGDQ"
     gene            3201..4178
                     /locus_tag="VFA54_06365"
     CDS             3201..4178
                     /locus_tag="VFA54_06365"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZS50462.1"
                     /translation="MFSIYRSLGEIPAVFGPSAITVGNFDGVHLGHSRILREVAQIGR
                     EHGWATLALTFDPHPTKVVAPDRMPRLLTSMPARLALFAQAGLQAALVLPFTPEVARL
                     APEEFVEKILVEKLRAQAVVVGANFRFGRNHAGDAALLGACGRRFGFEVRIIDPVIWR
                     GKIVSSSAVRQLISAGAVSQAAHMLGRPFAVEGRVVRGHGVGARQTVPTLNLAPETEV
                     LPERGVYSTCTTDPDSGREWPSVTNIGYRPTFGGSDLSIETFLLRPLDGPSPVRLSIA
                     FWRRLRDEKKFASPDELRAQIWKDVAATEKFFRRLRAAQKTTIAKEVVS"
     gene            4175..5197
                     /locus_tag="VFA54_06370"
     CDS             4175..5197
                     /locus_tag="VFA54_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HZS50463.1"
                     /translation="MKLSPGKQKGLEAVSDPRGVIAAAAMDQRGALRTPLAKARGAKP
                     EEVSDREMSEFKSAVTRVLSPHASAILLDPEWGLEAAKLRDKNCGLLLAYEKSGYDNT
                     QPGRRPDLLPLISARRLKEMGADAVKVLLYYTPEEAAAINDAKHAFIERIGAECAAVD
                     IPFFLEFVGYDPAGGDEKGFEFAKKKPEIVARSMAEFSKDRYLVDVLKVEIPVNLAFA
                     EGAKSFTGPKAYTKEEALEHYRRAAECAGRPFIYLSAGVSNEQFTESLRWAAEAGVKF
                     SGVLCGRATWKDGIPLYAKGGVEALENWLRKDGVANIKAVNECLKAATPWYAFYGAHS
                     AAEVAS"
     gene            5343..6734
                     /gene="cpaB"
                     /locus_tag="VFA54_06375"
     CDS             5343..6734
                     /gene="cpaB"
                     /locus_tag="VFA54_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZS50464.1"
                     /translation="MKRRLVPLLGVAFVVAVISTGVFYGLIVGKMRTSSGQPRPIVVA
                     AGSLPRGTVLDKTHVKLMPLSGRAVPEGAFTDPDQVTGLTVLQNIEPDQPITQASLSS
                     RDSAAPSAQAIPEGLRAVSIRVTDSPGVVAMLHAGYKVDVQAVRSRGSGETELHTVLQ
                     DVEVLTVQPESAQNRSGPGAVITLLAKPEDADVLGVADAGGRIRLVLRNPSDRQRMPL
                     PSLPFPALFHPQAGPGLSPVLRAALPVKTAAASPKAAAPAGPVPDEAAAQVQLRVQIV
                     AVSPSAAEELRSRLAPPARRDLLQVCAFRPGWSLESAVESLRAKRQIEVLSASTLLAG
                     NNHGVSMQAGAHSAGSTPAGAEETGVRIQFVPSFNRNGTFRIRVAPEVTSSGREGVTT
                     RKIETEVELSDGQGFLVAGLADSQAGSSLISRLFPGRTPEAGAGELLVLATPRLVKTV
                     QPERRSAALIQRP"
     gene            6747..7709
                     /locus_tag="VFA54_06380"
     CDS             6747..7709
                     /locus_tag="VFA54_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZS50465.1"
                     /translation="MLVISTILVVFVATFLAAAIAVLIASIILQRRSGESPENWAAMF
                     GETSASPLLKSEELSTVSLLSSLLARFDFVKVMKAQLEEADLRWPVGRLTLMMLLGGS
                     LMGALLGNASFLPSWVALSGGCAGGFLPYAYVLHKRKARLARFEEQLPDALDFLSRAL
                     RAGHPFAACLEMLADESQPPLSAEIRKTCDERQLGMSWEQALDNLATRVPLIDIGFFA
                     AAVQLQSRTGGKLGEVLGRLAETMRERFALRGEVRAIAAHGRMTGIVLTLIPVVVVSV
                     MAVVNPGYLEILLGSSTGRNLLFASIGCLVLAHFVIRRIVNIKT"
     gene            7706..8641
                     /locus_tag="VFA54_06385"
     CDS             7706..8641
                     /locus_tag="VFA54_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZS50466.1"
                     /translation="MTTELWLLIGFFAFVMLVVTGAGYAFVLRPADGSGSRPSESGLL
                     QGSEPEPASTKDILARIVRHLGEAVPAKQGEVSALRKRLISAGYRSPSTVPLFYGLKY
                     AGALLLGILIGWAVVLVREDASAALLPAIAGAGFGYLLPDRILRSMIAGRQERIHAAL
                     PDALDLLVLCIEAGQSLDQALIDASTELKRACPDLSSELALTHFELRAGATRAEALRN
                     LARRNPDPELRKLVNVLIQSDRFGTSLGPALRVHARYMRIRAKQKAEEAARKISIKLL
                     FPIFFLIFPCMLMVTAGPAVVQIFTQLLPMISGGQ"
     gene            8831..>9171
                     /locus_tag="VFA54_06390"
     CDS             8831..>9171
                     /locus_tag="VFA54_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50467.1"
                     /translation="MDILIVDDSVVLRKILERSLRQTQIAIDNVLEAGDGQEALEKLH
                     AHAVQMVFSDVNMPNMDGLELLRQIKATQSLRHVPVVIVTTDGSESTVLQALSLGAAA
                     YIRRPFTALQM"
BASE COUNT         1606 a         2862 c         2899 g         1804 t
ORIGIN      
        1 caggcttctg tcttccgccg caacggcgct ctgctcagca gcttcgggcc gttgctggcg
       61 gcgatgcatc ccgccggcga tgtgcccttg cgccctgtgc cgcgcggggg agaaaggtcc
      121 tttccgctgc tgcggctggc gatgctggcg tcggacgcgc gcggcccggc tctaatttcc
      181 gcgctcaatt tcagcgagac ccgccctgta gatagcgata ttcagaccgc tgatccgcgc
      241 acgggcagac cgctcgtgct gcgcggcttg aatctcccgc cgcggcaggc gctgctgatg
      301 ccggtcaatt ttcctttgtc gcagcccgag atatgcggtg actgctcttc tttcgctccg
      361 gatgagcgcc tggtttgggc gacggccgag ttgctctcgg tttcctttga aaacggcgtg
      421 ctggccatgg agttcgccgc gcccgccgag ggagagctga cactcgaact tgccagagag
      481 ccgaaagggc cgctgatcac gggcggtcac atccgcccgt ttgactggga cgagaagacg
      541 catcgattgc acgttcgcat tcccgccggt ccggccccgg agtatcgcac gcgggtcggc
      601 ttggccatcg atctgccgga tagctctgtt tttttgaaga cagcccggcg tctggtcatt
      661 ggttcgacgg ccaaagtgac ggctgtgttt tctccgccgg cgctggcgcg ccgctcgcgg
      721 cttctggcgc cccccggatg gcgcctcaaa gccgacccgc ccgggaaaga cgcgttgccg
      781 ggcgagattg attacagcgt cgttgtcccg gccggcgcgg tggccggaga tactgtgaca
      841 ctggccgtgg aagccgacgg caaaatcgtc caggctgcca cgctgccgct tgctcccttc
      901 tgcacggtgc gcatcgagcc ggaagaagcg ctgcacccgc ggcccgatga acggttcgca
      961 attcgcccgt acctcgccgc gctccacctg ccgggccggc gtgcgtatcg cgtgctgctg
     1021 cgcaacaact atgacgagat tcgaaatttc gagatcaccg cttccggcga gggactggtt
     1081 ttttctccat cccatctgga agtcagcgta ggcggcggac tggagcggca aatcacgttc
     1141 tatgcagcgc cggccggcgg gtccggccag aatcccggaa ccgatgactc ccccgggcgg
     1201 ggaaacagcg gctccgagcg ttcgcggacc ggcctctacc gcgggaacct ggagattcgg
     1261 cagggatctc agaagagcaa cgtcccgctg gcgctcgcgg tgattgctcc aggcgaatcg
     1321 ctcgcctatg aattcgacat ggaccgggac ggatccccgg aactggtgct cgaaaatcgc
     1381 aaactgcgcg ccgtcttttt tccccgccag ggcgggcgct cgacggaatt cctgctgaaa
     1441 gacgagggca tcaatgcgtt ctcgatacgc ggcgctctgg atgccggctt tcccatggaa
     1501 gggcgcgtgc ttgggccggg gcgtatcgaa ttgcatggcg gcggccttac gcgaatcatc
     1561 tcgcttggcg gagaagattc ctatttcgag gtggaagaaa ctggccgctc aggcgactgg
     1621 ttagtttcgg cggcgccgga cctggcctgg gccgggccca agggccacgc gcgttttgcg
     1681 attgatgttc ccaacgcgag gattgaggct gatcgcaagc ctttctcgat caactaccgc
     1741 gtgcaattcg cagacctccc cggccccggt tcccggagcg cccgcttcct tttcgagtgg
     1801 atcgaagagc acgccagcca gccggggaag taaacgtcgt tccactcaaa attcgggaag
     1861 cgcgggcgct accgaaccgc ggttcgtttg gttggcctct ccggttccgt cgcaaagacg
     1921 agggccgccc gccggacgca cttccggtca aacgccctgg gctacaataa tttctatgga
     1981 taaaggagag catgaaccgg aggtgaagat caccgatcgg cgccggtttt cgcgggaagg
     2041 ggaacccttg cagccggaag aagaagacgc cgcggaagaa aaaacggagc gggctgctga
     2101 gcccgccgct ccgccctccc agccggcagt caaccatact ccgctgccgc ctcccacatt
     2161 cgatgtgctg gtcctttcct tgcggtggca ggcggagctc caactcggca tgttcagcga
     2221 tgacaaagag caggagccga tgccggacct ggagggcgcc cggcactcaa ttgatctcct
     2281 gggcatgttg caggaaaaaa ctcggggaaa cctgaccctg gaagagcagc ggctgctcga
     2341 aaactcgctg accgaactgc gttttcgcta cgtcgaggct gtggaacggt tcgcgaaaaa
     2401 gaaataaatg ccagccaacg gaacagtgca gattaccgtg ctcggctcgg gaaccagcgt
     2461 aggcgtgcct acgatcgggt gcgactgccg ggtgtgccgc tccgacgacc ctcgagacca
     2521 gcggctccgc ccgtcgatat tggtcagcta caacaaccgc aacgtggtga tcgacactgg
     2581 ccccgatttc cgcatgcaag ctctgcgggc gggctttcgc accctggatg cggcgctgta
     2641 cacgcacgct cacgccgatc acatcctggg cctcgatgac gttcggccgt tcaactacgg
     2701 ccattccgag aacatcccca tctatgcgcg gccggaagtg ctgacggtta tccggcgggt
     2761 gttccgctac atctttgagg aaaccaacca ttgcggcggg attccaaggg tggaggcatg
     2821 ccccctggat gactccggag tggagctgtt cggccttcgg ttcataccgg tgccggtgct
     2881 gcacggggag cggctgatcg tgggatttcg ctttgggcgc gccgcgtatc tgaccgatca
     2941 cagcgatatc ccggagcagt ccatgccgct gctggagggc ctggacgtcc tttttctgga
     3001 tgcgctgcgc cacgtgcctc atccgacgca ctcgacggtg gagcgctcgc tgcgctgggt
     3061 ggagcaactg cgtcctgccc gcgcctactt cactcacatc tgccacgagc tcagccatga
     3121 agagaccgac cgctcgctgc cgcccaacgt ccgcctggcg tatgacggtc tccggatcct
     3181 cgtgggaggg gaccagtaga gtgttttcga tctaccgctc gctgggcgaa atacccgctg
     3241 ttttcggccc cagcgccatt acggtcggca attttgacgg cgtgcatctg ggccattccc
     3301 gcattttgcg cgaggtggcg caaatcgggc gggagcacgg atgggcgaca ttggcgctca
     3361 cgtttgatcc gcatccgacg aaagtggtgg cccccgaccg gatgccaagg ctgctgacat
     3421 ccatgcccgc gcggctggcg ctcttcgcgc aggcgggcct gcaagcggcc ctggtgcttc
     3481 ctttcactcc ggaagttgcg cgcctggcgc cggaagaatt tgtagaaaaa attcttgttg
     3541 aaaagctgag ggctcaggcc gtcgtcgtgg gagccaattt tcgctttggg cgcaatcatg
     3601 cgggcgatgc ggcgctgctg ggcgcctgcg gccggcgttt tggcttcgag gtgcgcatca
     3661 tcgacccggt gatatggcgc ggcaaaattg tctccagttc cgcggtccgg cagcttatca
     3721 gcgccggggc ggtctcgcag gcggctcata tgctgggacg tccttttgcc gtggaagggc
     3781 gggtcgtgcg cggacacggc gtaggcgccc gccaaacggt tcccacgctg aatctcgcgc
     3841 ctgaaactga agtgctgccg gagcgcggcg tctacagcac ctgcacgacc gatcccgatt
     3901 ccggccggga atggccttcg gttacgaaca tcggatatcg ccccacgttt ggcgggtcag
     3961 atttgtccat cgagaccttt ctgctccgtc ctttggacgg accgagtccc gtccgcctgt
     4021 cgatcgcgtt ttggcgccgc ctgcgggacg agaaaaagtt cgcctcgccg gatgagctcc
     4081 gggcgcagat ctggaaggat gtcgctgcta cagaaaaatt ctttcgccgc ctgcgtgcgg
     4141 ctcaaaagac caccatcgca aaggaggtag tttcgtgaag ctctcgccag gaaaacagaa
     4201 aggcctcgag gcggtttcag atccccgcgg cgtgatcgcc gccgcagcca tggatcagcg
     4261 gggcgcgctt cgcacgccgc tcgccaaggc gcgcggcgcg aagccggagg aagtgtccga
     4321 ccgggagatg agcgagttca agtcggcggt gacgcgggtg ctctctccgc acgccagcgc
     4381 catccttctc gatccggagt ggggcctcga agccgcgaag ctgcgggata aaaactgcgg
     4441 cctgctgctc gcctatgaaa agagcggcta tgacaacacc cagcccggcc gccgccccga
     4501 tcttctgccg ctcatctccg cgcgccgcct gaaagagatg ggcgctgacg cggtgaaggt
     4561 gctcctgtat tacacgcccg aggaagccgc cgccatcaat gacgcgaagc acgctttcat
     4621 cgagcgcatc ggcgcggagt gcgcggccgt cgatattccg tttttcctgg aatttgtcgg
     4681 ttacgacccc gccggcggcg acgaaaaagg atttgaattt gccaaaaaga aaccggaaat
     4741 tgtggctcgc agcatggcgg agttttccaa agaccgttat ttggtcgacg ttttgaaggt
     4801 tgaaatcccg gtcaatctcg cgttcgccga aggagccaaa tcgtttaccg gcccgaaagc
     4861 gtacacgaaa gaagaagcct tggagcacta ccggcgcgct gcggaatgcg caggccgtcc
     4921 gtttatttat ctcagcgccg gcgtcagcaa cgagcaattt acagaatccc tccgctgggc
     4981 cgccgaggcc ggcgtcaaat tctcaggtgt gctctgcggc cgcgccacct ggaaggacgg
     5041 catcccgctc tacgcaaaag gcggcgtcga agcgctcgag aattggcttc ggaaagacgg
     5101 tgtcgccaat atcaaagcag tcaacgagtg cctgaaagcg gccacgccct ggtacgcgtt
     5161 ttatggcgcc cattccgccg ccgaggtggc gtcataagcg cgcgaagccg gctggaacta
     5221 cgacaaaaat cctagaggcc tagtagtaaa gcccaattct gctctttggc ccgccgtgtt
     5281 gtacttctag ttgcagctcc ccgggagtgg tttgtcttca ccgggctgcg cggcggattt
     5341 ttatgaaaag aaggctcgtg cctctgttgg gggtcgcatt tgtggtcgcc gtaatctcga
     5401 ctggcgtttt ctatgggctg atcgtcggga agatgcggac atcttcgggc cagccacgtc
     5461 cgatcgtggt agcagcgggg tctctgccgc gaggcacggt tctcgacaag acgcatgtca
     5521 agctgatgcc gctttccggg cgcgccgttc ccgagggagc tttcaccgat ccggatcagg
     5581 tcacgggcct gacggtcctg cagaacatcg agccggatca gccgattact caggccagcc
     5641 tttcctcgcg cgattcggcc gccccttcgg cgcaggcgat accggagggc ttgcgggcgg
     5701 tttccattcg ggtgaccgat tcgcccgggg tggtcgcgat gctgcacgcc ggctacaagg
     5761 tcgatgtgca agcggtccga agccgcggca gcggtgagac ggaactgcac acagtgctgc
     5821 aggatgtaga ggtgttgacc gtgcagccgg agtccgccca aaaccgcagc gggcccggcg
     5881 cggtgattac gctgctggcc aagccggagg acgccgatgt gctgggcgtt gcggacgcag
     5941 gcgggcgcat tcgcctcgtc ttgcgcaatc caagcgacag gcaaaggatg ccgttgccga
     6001 gccttccatt cccggctctg ttccaccctc aagctggtcc cggtttgtcc ccggtgctgc
     6061 gggctgcgct gccggtgaaa acagcggctg catccccgaa ggcggcggct cccgccgggc
     6121 cggttccgga cgaggccgct gcccaggtcc agcttcgtgt ccagatcgtc gcggtttcgc
     6181 ccagcgccgc cgaagagctc cgctcgcggc tggctccccc ggcgcgccgg gatttgctgc
     6241 aagtatgcgc tttccggcca ggctggagtc tggaaagcgc ggtggagagc ctgcgcgcaa
     6301 aacggcagat cgaggtcctc tcggcctcca ctctcctcgc tggcaataac cacggagtca
     6361 gcatgcaggc gggcgcgcat tctgccggct ctactccggc aggcgcggaa gaaaccggag
     6421 tgagaattca gtttgtgccc tccttcaacc gcaatggcac cttccgcatt cgcgtggcgc
     6481 cggaggtcac atcttccggg cgcgagggcg tcaccactcg caagatagaa accgaagtcg
     6541 agctctccga cggccagggc ttcctggtgg ccggcttggc ggacagtcag gcaggaagca
     6601 gtctgatatc ccggctcttc ccgggccgca cgcccgaggc cggagcaggc gagttgctgg
     6661 ttttggcgac cccgcggctg gtgaagacgg ttcagcccga gcggcgatcc gcagctctga
     6721 tccagcggcc gtaagcacaa acgccgatgc tcgtcatatc cactatcctg gtcgtctttg
     6781 tggctacgtt cctggcagcc gccattgccg tgttgatcgc ctcgatcatc ttgcagcgcc
     6841 gctcgggcga aagccccgag aattgggcag cgatgttcgg ggagacctcc gcgtcgcctc
     6901 tgctcaaaag cgaagagctg agcacggtct cgctgctttc cagcctcctg gcgaggtttg
     6961 atttcgtaaa ggtgatgaag gcgcagctcg aggaagcaga tctgcgctgg cctgtcggac
     7021 ggctgaccct gatgatgctg ctgggcggca gcttgatggg ggctttgctg ggaaacgcaa
     7081 gctttctccc gtcctgggtg gctttgtcag gcggatgcgc gggcggcttt ctcccctatg
     7141 cctatgtgct ccacaagcga aaagcgcgcc tggcgcggtt tgaggaacag cttccggacg
     7201 ccctcgattt tctctcccgc gcgctgcggg cgggccatcc gtttgccgcc tgtctggaaa
     7261 tgctagccga cgagagccag cctccgctgt cagccgaaat ccgcaaaact tgcgatgagc
     7321 ggcaattggg gatgtcctgg gagcaggctc tggataacct cgctaccagg gtgcccttga
     7381 tcgatatcgg cttctttgcc gccgctgtcc agttgcaaag ccgcactgga ggcaaactgg
     7441 gcgaggtgct cggcaggctc gccgagacga tgcgcgagcg cttcgccctg cgcggcgagg
     7501 tccgcgccat cgccgctcac ggacgcatga cgggtatcgt gctcacgctc ataccggtgg
     7561 ttgtcgtctc ggtcatggct gtcgttaatc ctggctactt ggaaatcctt ctggggagtt
     7621 caaccggcag aaacctgctg ttcgcctcca tcggctgcct ggttctggcg catttcgtta
     7681 ttcgccggat tgtgaacata aaaacatgac caccgaactg tggctcctga tcggtttttt
     7741 cgccttcgtc atgctcgtgg tcaccggcgc cgggtatgca tttgtcctgc ggccggccga
     7801 tggcagcggg agccgccctt cggaatccgg tctgttgcaa ggctccgagc ccgagcccgc
     7861 ctcgacaaag gacatcctgg cgcggatagt tcgccacctg ggagaagccg tgcctgctaa
     7921 gcagggcgag gtgagcgcgc tgcgtaagcg cttgatttct gcggggtacc gctcaccctc
     7981 cacggtaccc cttttttacg gtctaaaata tgcgggcgca ttgctgctgg gaatcctcat
     8041 aggctgggcg gtcgtgttgg tccgcgaaga cgcttccgcg gcgctgcttc ccgcgatcgc
     8101 cggggcgggc ttcggctacc tgctgccgga ccgcattctg cgctcgatga ttgcgggccg
     8161 gcaagaacgc attcacgccg ccttgcccga cgccctcgat ctgctggtac tctgcattga
     8221 agccggacaa tccttggacc aggccctgat cgatgcgagc acggagctaa aacgagcctg
     8281 cccggacttg agctccgagc tcgccctgac ccacttcgaa ctgcgagccg gcgccactcg
     8341 agccgaggcc ctcagaaatc tcgcccggcg caatcccgac ccggaactgc gcaagctcgt
     8401 gaacgttctc atccagagcg accggtttgg aaccagcctt gggccggcgc tgcgggtcca
     8461 cgcccggtac atgcggattc gagcgaaaca gaaggcagaa gaggccgctc gcaaaatcag
     8521 catcaagctt ctcttcccga tttttttcct gattttcccg tgcatgttga tggtcacagc
     8581 cgggccggcc gtcgtgcaaa tcttcaccca gctcttgccc atgattagcg gcgggcagta
     8641 agcggaaacg gtctcttcgc cgccggtttt ccggcactta gaaaactttc gccagagttt
     8701 ccggcctgtt cttcccgctg gcatctcaca cctgcccctg gatcggcggc ggtaagggcg
     8761 ccatcttcta ccgttgccgc ccgggttcgc cgattgctta tttgaggtcg cggcggcagc
     8821 ccaggcatct atggatatcc tgatcgtcga tgattcagtg gttttgcgaa aaatcctcga
     8881 acgaagcctg aggcagactc agatcgccat cgacaacgtg ctggaggccg gcgacgggca
     8941 ggaagccttg gaaaagctgc acgcccatgc agtacagatg gttttttccg acgtgaatat
     9001 gccgaacatg gacggcctcg agctcttgcg ccagatcaaa gccacccaga gcctgcggca
     9061 tgtcccggtg gtgatcgtga caacggatgg atccgaatcg acggttttac aggctctttc
     9121 cttgggcgcc gccgcttata tccggcggcc cttcaccgca ctgcaaatgg a
//
LOCUS       DASREK010000129         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_737877, whole genome shotgun sequence.
ACCESSION   DASREK010000129 DASREK010000000
VERSION     DASREK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737877"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFA54_06395"
     CDS             <1..258
                     /locus_tag="VFA54_06395"
                     /inference="COORDINATES: protein motif:HMM:NF023298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HZS50468.1"
                     /translation="RGAIGVQAAGWERIAFAVWAWLMRRPRLFELAGSAAQVLLPADR
                     DGGWIRSLPGLLQAWTRWRDFPPPAAHSFRQLWRRQRRRSN"
     gene            262..888
                     /locus_tag="VFA54_06400"
     CDS             262..888
                     /locus_tag="VFA54_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HZS50469.1"
                     /translation="MPAREHILHKIRTALGRNAGQPPAPAPPPRLVFAAASSAELVRQ
                     AIERFPGKAVRAGSDQEARDYVASQINGLRAIASPAPLLDRLGILTLPGVATAAGGPA
                     EVQRACAESDIGITTAEYVLADTGALVTLSGARESRLVSLLPPAHIAVAPADRILTGL
                     DELFTLLPDPAALSSSLVLIAGPSRTADVEGVLTLGVHGPKELHLVLV"
     gene            complement(993..1268)
                     /locus_tag="VFA54_06405"
     CDS             complement(993..1268)
                     /locus_tag="VFA54_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50470.1"
                     /translation="MKLLRVKVDIGGTRNEEAWTGMKHFEEVPPHEEIFRLFCGHDAN
                     QPHPAGEWGEATLKFEDFIAEYAIAHYLKQPRVIDANIEPDKAPLES"
     gene            1332..>3803
                     /locus_tag="VFA54_06410"
     CDS             1332..>3803
                     /locus_tag="VFA54_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50471.1"
                     /translation="MLALCDLCKSIRLCFLILLLGIYPVFGAAPPEGNEPRCGSDPAL
                     RLTDLFRRQQAQLRRTPAALGIGPAPAFLPDIGDIAMVDDSEGVVVRPNPFNLQQLTL
                     HFSPSGARASEYTAATGALHFDAAAAAAGTPLASLGDDDTRMVPLPFSFPFFGQRYTS
                     LYVNSDGNLTFTGGDASSDPRSAGRAISGPPRIFPFFDDLDPSQPGAAVEYFGAADRA
                     VVTWNQTPEFSETTPGARQTFQAVLYPDGRIDFNYQTIATGTAAVVGIAPGFQQSGFT
                     AVDFSAGVAVPRGGALAEVFSNRNSIDFIAVSQKFYRNHEDAYDYIILFNTLGFTGED
                     DPGAFSAERNIRNHVRGIGGLSRGNSVFDDGLSWGSPLRLQSFVNMGPLANFPANPAD
                     IIPAFASSRNTPLSILGQESGHRFLAYVRYLDPLTGQPSSSLLGRANAHWSFFFNSDA
                     SVVEGNRIQDLGTGQSPRFLTIGTVEHYSALDQYLMGLRGPDDVPPSFLVRNPSIPLP
                     AASAPQPGVRFDGARQDITLQMITDAEGKRIPDWTVAQKHFNFAFVLLAPAGSAPPAA
                     DVAKLEQLRRAWGSYFAAAVDNRGTAEVRLVKELLLSTWPAGGVVQGSPATTATVSVA
                     APLSSKLDVILSTDSSVIAIPPSVTIPAGAVSASFSIVGNRPGTAALSARAVDASYEV
                     AQTIVQVRESASQLHLDLVSGGGQQGALGQVLSEPIVLRLHDDNDVPFSGVPVNFAAS
                     GDGVVTPSRALTDAAGRVRVSWRLASSGASNTLTASPDGAASEGVGVSARGLAQPVFS
                     AAGVVNAAALQPAPAAAGISPGGLTT"
BASE COUNT          606 a         1216 c         1166 g          815 t
ORIGIN      
        1 cgcggcgcga tcggagtgca ggccgccggt tgggaacgga tcgcgtttgc cgtttgggcg
       61 tggctgatgc ggcgcccgcg cttgtttgaa ctcgccggtt cggcggccca ggttctgctg
      121 ccggcggatc gcgatggcgg ctggatccgt tccttgcccg gtctgctcca agcctggaca
      181 aggtggcgag actttccgcc gcccgccgcg catagcttcc ggcagctttg gcggcggcag
      241 cggcgccgca gcaactgacg catgccggcc cgagagcata tccttcacaa gatccgcacg
      301 gccctgggcc gcaatgccgg acaaccccca gctcccgcgc ccccgcctcg attggtcttt
      361 gccgctgcca gttcggctga gctggtccgc caggctatcg agcggtttcc cggtaaggct
      421 gtgcgcgccg gctccgatca ggaagctcgc gattacgtcg cttctcaaat taatggattg
      481 cgcgccatcg cctcgccggc gccgctgctc gatcgcctcg ggattctcac gctgccggga
      541 gtggccacag cggcgggcgg tccagccgaa gtgcagcgtg cctgcgctga gagcgatatc
      601 ggaatcacca ccgccgagta cgtcctggca gataccgggg cgctggtcac gctgtcgggc
      661 gctcgggaat cgcgtctagt ttcattgctg cccccggcgc atatcgcggt ggctccggca
      721 gaccgcattc tcaccggact cgatgaactc tttacgcttt tgccagaccc ggcggccctc
      781 tccagctccc tggtcctgat cgccggccct agccggaccg ccgacgtgga aggggtcttg
      841 accctgggcg tccacggtcc gaaggagctg cacctcgtgc tggtatagtg cccgggcctc
      901 tttgccatag ctgtttcttt gcagtttggt gatggcttcc tggtcccctt gcagttggac
      961 ctggggagcc gcacggcgag ggcttcccgg cgctaggatt ccaatggagc tttatccggc
     1021 tcaatattgg cgtcaattac gcggggttgc ttgaggtagt gtgcaattgc gtattcggcg
     1081 ataaagtcct cgaatttcag agttgcctcg ccccactcgc cagccggatg cggctggttg
     1141 gcgtcgtgac cacagaacag ccggaagatt tcctcatgag gcgggacctc ttcgaagtgc
     1201 ttcatgcccg tccaggcttc ttcgtttcgt gtgccgccaa tatccacctt cacgcgtagc
     1261 aatttcactg gattccctcg tttcccggtc ccaaacacgc tcccgttggt ccggtgataa
     1321 gataaaaagc cttgctggcg ctttgcgact tgtgtaaatc gattcggctc tgcttcctca
     1381 ttttacttct tgggatctat cccgttttcg gcgcggcgcc gccggaagga aatgaacctc
     1441 gctgcggcag cgatccggcg ctgcgcctga ctgacctgtt ccggcgccag caggcccaat
     1501 tgcgccggac tcccgctgcg ctcgggatcg gtcctgcgcc tgcctttctt ccggatatcg
     1561 gagatatcgc aatggtggac gactccgaag gcgtggtggt ccgccccaac ccattcaact
     1621 tgcaacagct cacgttgcat ttctcgccca gcggcgcgcg cgcttcggag tacacagccg
     1681 ctacaggcgc gctacatttc gacgcggccg cagcggccgc gggaacgccg ctggccagcc
     1741 tgggcgacga cgacacccga atggtgccgc tgccgttctc tttcccattc ttcgggcagc
     1801 ggtatacgtc cctctatgtc aactccgatg gaaacctgac gttcacgggg ggcgatgcgt
     1861 cgagcgatcc acggagcgca ggccgggcga tctccggtcc gccgcgaatt ttccctttct
     1921 tcgacgacct cgatccctcg caaccaggcg ctgccgtcga atactttggc gcggccgatc
     1981 gtgcagtggt cacgtggaat cagacgccgg aattttcgga aaccacgccg ggcgcccgcc
     2041 agacgtttca ggccgttctc tatcccgacg gccgcattga cttcaactac cagacgatcg
     2101 ccacaggcac tgcggccgtg gtcgggatcg cccctggctt tcaacagagc ggcttcacgg
     2161 ccgttgattt ctcggctggc gtagcggttc cacggggagg cgcgctggcc gaggtgttca
     2221 gcaataggaa ctcgatcgat ttcatcgccg tgagccaaaa gttttaccgc aatcatgagg
     2281 acgcctacga ctacatcatt ctgtttaata ccttgggttt tacgggagaa gatgatccgg
     2341 gagcattttc cgccgagcgc aacatccgca atcacgtccg tggaatcggc ggcctctcgc
     2401 gggggaacag cgtattcgat gacggcctga gctggggctc gccgctgcgc ttgcaaagct
     2461 tcgtgaacat gggcccgctt gccaacttcc ccgcgaaccc agccgatatt attcctgctt
     2521 tcgcttcgag cagaaacacc ccgctctcca ttctgggcca agaaagcggc caccggtttc
     2581 tggcgtatgt acggtacctt gatccgctga ccggccagcc gtcctcaagc ttgctcggcc
     2641 gcgccaatgc tcactggagc ttctttttca acagcgacgc gtctgtggtc gagggaaacc
     2701 gcattcaaga tctgggaacc gggcagtcgc cccgcttttt gactatcgga accgttgagc
     2761 actacagcgc tttggatcag tatttgatgg gcctgcgcgg tcccgatgat gtgccgccat
     2821 ctttcctggt gcgaaatccg tcgattccgc ttcctgctgc cagcgcgccg caaccgggcg
     2881 tacgtttcga cggcgcgcgg caagatatta ccttgcaaat gatcactgat gccgagggaa
     2941 agcgaatccc cgattggact gtcgcgcaaa aacacttcaa ttttgcattc gttctgctgg
     3001 cgcccgccgg ttccgcgccg ccggcggccg acgtggccaa gctggagcag ttgcgccggg
     3061 cttggggcag ttactttgcc gccgcggtgg ataatcgcgg cacggcggag gtgcggttgg
     3121 taaaggagct tttgctttcg acttggccgg cgggtggcgt agtgcagggg agtcccgcca
     3181 caacagccac cgtgagcgtc gccgctcccc tttcttccaa gctggacgtg attctcagta
     3241 cggacagcag cgtgattgcg attccgccgt cggtcacgat ccctgccgga gccgtttcgg
     3301 cttctttctc gattgtagga aaccgcccgg gaacagccgc tctgagcgcg cgcgcagttg
     3361 atgcctccta tgaggttgca cagacgattg tccaggtccg cgagagcgcg tcccagctcc
     3421 atctggactt ggtttccggc ggcgggcagc aaggggctct cggacaagtg ctctcggaac
     3481 ccatcgtgct gcggcttcac gatgataatg acgttccttt ttcgggagtg cccgtcaact
     3541 tcgctgctag cggcgacggt gttgtgactc cttctcgcgc cctgaccgac gccgccgggc
     3601 gggtacgtgt aagctggcgt ctggcatcga gcggcgcttc caatactctg accgcttcac
     3661 ctgatggggc ggcgtcggag ggggtgggtg tatcggcgag gggtcttgca cagcccgtct
     3721 tcagcgccgc tggcgtggtc aatgcggcgg cgttgcagcc agcgccggca gccgccggca
     3781 tttcaccggg cggcctaacg act
//
LOCUS       DASREK010000130         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_893216, whole genome shotgun sequence.
ACCESSION   DASREK010000130 DASREK010000000
VERSION     DASREK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893216"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="VFA54_06415"
     CDS             <1..1689
                     /locus_tag="VFA54_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50472.1"
                     /translation="GGGMGRDLSDMFNLELDTEKNQYETGQQASAQERDRELDQALEK
                     LRELARRQEQLAEQQRRQQIPNFDQRWQQEMLRREAEELTRQLQRMQQQQQNGRQSAQ
                     GRQGGQQVNSSSSSQASNSAIQRTLERLQAATRDMQQGSQSGQNGQAGSDAERRAAIA
                     RAQERLKEAEQLLSGERQQRAGEQLEDMEQRASELASRQQALANKLRDSFRQSNEGLR
                     DPVTGRSIYGQDGLQGGPSRQEAQAMAREEEQVREQLQALEKQMQEAARRLAENQPKT
                     SRSLRNALSNMQQAEPFRDLGLASDYLKRGLGPYALQREQMAARALNQLRDGLQETRR
                     LAQNEEKGQGGEGLEQALNRVEQLRQQIERAMAARQAQQQGQGQQQGQQQGQAGQQGQ
                     GQQGQGQQQGPGQQAGGAQQGGGGPYGGWGPNGGYWRGGAWAGWNGRDILPPGRFPDP
                     IDPNRVFREAPAELSQIEGSLRNDPDLAREVEELRKQLRMLPGNRPQGGRDLSVLTAE
                     QQKLLQEAEDLELLLRRKLDEKQGSQVRAITDQPIPEGYRKAVAEYFRRLSREK"
     gene            complement(1778..2395)
                     /locus_tag="VFA54_06420"
     CDS             complement(1778..2395)
                     /locus_tag="VFA54_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZS50473.1"
                     /translation="MTKALLFDLGNVLIGFDFARGYRAMQAICGHTAEEIRRMINHAE
                     LVHPYERGEISSGEFYERLCGVLSLKVSYRQFCDLWSEIFLPEPLLPVELLAALRRRY
                     RMVLVSNTNDIHFEMIRRQHEALIRHFDARVLSYCVGASKPSERIYQEAVRQARCDPG
                     ECFFTDDMPEFVEAARRYGIDAVQFQGYSRLQQELRKRGINWDGA"
     gene            3252..6206
                     /locus_tag="VFA54_06425"
     CDS             3252..6206
                     /locus_tag="VFA54_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50474.1"
                     /translation="MKNIISLTIRLFVCTAALLLGASPALFAQGEIASATLNGTVTDP
                     SGSAVPNAKITLTNTATGLTRTTQSNDAGFYSFVRVPVGVYDLTIEAAGFRTSKRTGV
                     SLAVGAVATLDISLEIGATQETVTVSATDAPLIETTRSQTSTVINERAVAELPLNGRN
                     FLDIAVSTPGVTRDPTRTGDLSFGGQRGTSNSLLVDGADSNNVFFMQSTGRAGTGRNP
                     YSFSQDAVQEFQVNTSGYNAEIGRAGAGVINVITKSGTNDVHGTVFEFYRDKALNANT
                     WENNRRGIPKRAYHFNQFGGNIGGPIVKNKIFFFFDYDGQRNTTPNPVFLQVAPPSDA
                     LSQQAFQSLQKYLGAYSNGLNNDVYLGKVDWDLTGRQRLSVRYNANRFVGQNFENSGP
                     ASALEHTGNSDVTTDNLAASHTFVINPTTVLESRFAFTRDDEPGFANSSAPEAVIRQN
                     GIAVLSIGRNSFSPRYTNDRTYQWLESLSRVLGRHSLKFGADLSFDRIANFFPGNFSG
                     SYVFNSYADFAANNPFSFTQAFAGPGTDGPLSQPNVGEYAFYAQDSWRITDKLTLNYG
                     LRYDYFNYAQPKVKNPDPGLAALKLDTSRIHLDSNNLAPRFGFAYKLDRSGNTVIRGG
                     YGMYYGRTPSILTGTAITTNGIQVQSYTLFSGIPTYPNILPAPPPLSRTPDIFLFAND
                     YVQPLTHQWSVNIDRQLGRSYALTFGYLGVRGEHLSRTRDINLLPAVAVQGSFADGTP
                     VTFFRHPGRANPNFGRISLVDSGADSIYHGGFVQLTKRFSHNFQVQTSYTFSKVIDDD
                     PDQTSVVVGTDDAKNAQDTLQPNLERGRGNADIRHRFVLSGVWNIDYANSLQNSVLRA
                     LLRGYELSTITTVQSGRPFTATVGADVNNDGNTRTDRPPYVGRNTINGPNFADVDLRF
                     SREIGLYERLRMRLIFEAFNLTNRVNFDTILATQYNFNATTRVFSPASTFLQPSTTIA
                     PRILQLAAKITF"
BASE COUNT         1354 a         1899 c         1883 g         1150 t
ORIGIN      
        1 ggcggcggca tgggacggga tctctcggat atgttcaacc tcgagctgga caccgagaaa
       61 aaccagtacg agaccggcca gcaggcctcc gcccaggagc gcgatcgcga gctggatcag
      121 gccctggaga agctgcggga actggcccgc cgccaggagc agctcgccga acagcagcgc
      181 cggcagcaaa taccgaattt tgaccagcgc tggcaacaag agatgctgcg gcgcgaagcc
      241 gaggaattga cccgccagct ccagcgcatg cagcagcaac agcaaaatgg ccggcagagc
      301 gcccagggcc ggcaaggcgg ccagcaagtc aacagcagta gcagcagcca agcctctaac
      361 agcgccatcc aaagaaccct cgagcggctt caggcggcta ctcgcgatat gcagcaaggg
      421 tcccaatcag gccagaacgg ccaggctggt tccgatgccg agcgccgggc cgccattgcc
      481 agagcccagg agcggttaaa agaggccgag caactgctct ctggggagcg ccagcagcgg
      541 gcgggggagc aactggagga catggagcag cgcgccagtg aactggcatc gcggcagcag
      601 gcgttggcaa acaagctgcg cgactccttc cggcagtcca atgaaggcct ccgcgaccca
      661 gtgacgggcc gctccattta tggccaggac gggctgcaag gcgggccatc gcgccaggaa
      721 gcccaggcaa tggcgcggga agaggagcag gtgcgggaac agctccaggc cttggaaaag
      781 cagatgcagg aggctgcgcg gcgtctggcg gaaaaccagc cgaaaacttc ccggagtctc
      841 cgcaacgcgc tcagcaacat gcagcaggct gaaccgttcc gggacctcgg cctggcatcc
      901 gactatttga agcgcggcct gggaccctac gctctccagc gggagcaaat ggctgctcgg
      961 gctttgaatc agcttcgaga cggtctccag gagacgcggc gtctggctca gaatgaggaa
     1021 aaaggccagg gcggcgaggg tctggagcaa gcgttgaatc gcgtggaaca gctccgccaa
     1081 cagattgagc gtgccatggc ggcgcggcaa gcccagcagc agggccaagg tcagcagcag
     1141 ggccagcagc aagggcaggc tggccagcag ggtcaagggc agcaaggtca aggtcaacag
     1201 caggggccgg gccagcaggc ggggggcgcc cagcagggcg ggggcgggcc ttacggaggc
     1261 tgggggccaa atggcgggta ctggcggggc ggcgcatggg ccgggtggaa cggccgtgac
     1321 attctgcctc ctggccgttt ccctgatccg atcgatccta accgtgtctt tcgcgaggcc
     1381 ccggctgagc tttcgcagat cgaaggcagt ctcagaaacg atccagatct ggcccgcgaa
     1441 gtagaagagt tgcgaaagca gttgcgcatg ctgccgggca accgtccgca gggcggccgg
     1501 gacttaagcg tgcttaccgc cgagcagcag aagctgctgc aagaagcgga agacctggag
     1561 cttttgctcc gtcgtaagct cgatgaaaag caggggtcgc aggtacgggc gataaccgac
     1621 cagccgattc ctgagggcta ccgcaaggcc gtggcagagt acttccgccg gctgagcagg
     1681 gagaaatagc gatcgctcag gccacatagc gcgggaacag cgagaggcaa ccgggattcc
     1741 tcccctacga acgggtcagg tgtagacttg ccggccttca ggccccatcc cagttaatcc
     1801 cccgtttccg caattcctgt tgcaaccgcg aataaccttg gaactggacg gcatcaatgc
     1861 cataccggcg cgcggcctcc acgaattcgg gcatgtcatc ggtaaagaaa cactcgcccg
     1921 ggtcgcaccg ggcctggcgg accgcttcct gataaatgcg ctctgacggc ttggatgcgc
     1981 ccacgcaata agaaagtaca cgcgcgtcaa agtgccggat tagggcttcg tgctgcctgc
     2041 ggatcatctc aaaatgaatg tcgttggtat tggagacaag caccatccga tagcgccggc
     2101 gcagagcggc taaaagctcg accgggagca agggctcagg cagaaagatc tcgctccaaa
     2161 gatcgcagaa ctgccggtaa gacaccttga ggctcagcac gccgcacagg cgctcgtaaa
     2221 actccccgct cgaaatttcg ccgcgctcgt aaggatgcac tagttcggca tggttgatca
     2281 tgcggcgaat ctcctcggcc gtgtgcccgc aaatcgcttg cattgcgcgg tagcctcgag
     2341 cgaagtcaaa accgatcaga acgtttccga gatcgaaaag aagggctttg gtcatggttc
     2401 agttacagaa ttcgagcaca tgcgcatcgg ctccggccgg gatcaaaaca acaggcgatt
     2461 ttccaggaac ccacccggcg aactaactcg ggcgctcagg gcgccgaaga ggcgccggtt
     2521 tgcaaaaggg gaagccggct cgtgagatcg gcggcattgg gggaaacccc gccgaccgcg
     2581 cgagctacgg aggatgatgg aacacgccgg ccggcctctt tgggctattt tcagaacttg
     2641 cgaggtcgag ttgccgtctt ttcgttcttg gacgccccat gtgcagtctt cagcgattgc
     2701 ctcatcggct gagcggcaca tagaaccccc gaacctgcca tcgccctcca cacccgatcc
     2761 ataccaagcg ccgcccatcc attcctcatt gtaaatcgca gtaattcaga aatcgtctct
     2821 ggcaaccagg catctcccca gtggtttcct ggcggttcct cctgacaccg gcttacttcc
     2881 gctgggcccg cccttcggcg ccgcgtttcg ccaggctaat ccaatggcat tcgctgacgg
     2941 acggctgggg gcaagaggca cacgtcgccg ccgtgctaac gagggctgtc tcttgtcctt
     3001 aggatgagcg aggcatgtgc ttttttgccc ctgggcaacc cctaaacagc aggtcctttc
     3061 ttctgcccgg acgcggcgga tctctctata ccgcgatgat tcatcacgtt cctattcaag
     3121 cagccagttg tcacaaagga ttcacttgac tcgttcagtg ttccgggcga tccttaaaaa
     3181 gctggaactg acttgggaag gtgacttggc cctttcagga gggttccgcg cgtcgcaggg
     3241 taatcttcgt tatgaaaaac atcatttctc ttacgatccg gctctttgtg tgtacggcgg
     3301 cgctgctcct gggcgccagc ccagcccttt tcgctcaggg agaaattgcc agcgcgacat
     3361 tgaatgggac cgttaccgat cccagcggct ccgctgttcc caacgcgaaa attactttga
     3421 ccaacacggc gacgggattg acacgcacta cccaaagcaa cgacgccggc ttttacagtt
     3481 tcgtgcgggt gccggttggg gtatatgatt tgacgatcga ggcagcgggc ttcaggacca
     3541 gcaagcggac aggcgtttcg ctggccgtcg gcgccgtcgc gaccctggat ataagcctcg
     3601 agatcggcgc tacgcaggaa accgtaacgg tcagcgccac tgacgcgccg ctgatcgaga
     3661 cgacacgctc gcagacttct acggtcatca acgagcgggc ggtcgccgag cttccgctca
     3721 acgggcgaaa tttcctcgac attgccgtct cgactccggg cgtgacgcgg gacccgacgc
     3781 gaaccggaga tctttccttt ggcggccagc gcggaacgtc caacagcctc ctggttgacg
     3841 gcgcggattc gaacaatgtc ttcttcatgc agtccaccgg gcgagccgga accggccgca
     3901 acccttacag cttcagccag gacgccgtac aggaatttca ggtaaatacg agcggctaca
     3961 acgcggagat cgggcgggcc ggcgcgggtg tcatcaacgt gattacgaaa tcagggacca
     4021 atgatgttca cggaacggtg ttcgagttct atcgggataa agcattgaac gccaacacct
     4081 gggaaaataa ccggaggggc atccccaagc gggcttatca cttcaatcag ttcgggggca
     4141 atatcggcgg ccccatcgtg aagaacaaaa ttttcttctt tttcgattac gacggccagc
     4201 gcaacacgac gccgaaccca gtctttctgc aagtggcgcc gccttccgac gcgctctccc
     4261 agcaggcctt ccagtccctg cagaagtatc tcggggccta ttctaacggg ttgaacaacg
     4321 acgtgtacct gggaaaggtg gattgggatc tgacgggccg gcagcgcctg agcgtccgct
     4381 acaatgccaa ccgcttcgtg ggccagaact tcgaaaatag cggcccggca agcgcgcttg
     4441 agcatacggg caactcggat gtcacgacgg acaacctggc ggcaagccac acttttgtca
     4501 tcaacccgac cacggtactc gaatcgcgct tcgcgtttac tcgagacgac gagcccggat
     4561 tcgccaactc ctcggccccc gaagcggtca tccgccagaa cggaatcgcg gtgttgtcca
     4621 ttgggcgcaa tagcttcagc ccaaggtaca ccaatgacag aacgtatcag tggctggaaa
     4681 gcctctcgcg cgtgcttgga cggcactcgt tgaagtttgg cgcagatttg agctttgacc
     4741 gcattgccaa ttttttcccc ggaaacttca gcggttctta cgtcttcaac agctacgcgg
     4801 atttcgcggc caacaacccg ttcagtttta cccaggcctt cgccggtccc ggcactgacg
     4861 gcccgctctc gcagcccaat gtcggcgaat atgcgtttta tgcccaggat agctggcgta
     4921 taacggataa attgacgctg aattacgggc tgcgctacga ctatttcaat tacgcgcaac
     4981 cgaaggtgaa aaacccggat ccgggattgg cggcgctgaa actcgatacc tcgcgcatcc
     5041 atctcgactc gaacaatctc gccccgcggt tcggtttcgc ctataagctg gataggagcg
     5101 gcaatactgt catacgcggc ggatacggca tgtactatgg ccggacgccg tcgattctga
     5161 ccggcactgc aatcaccacc aacggcatcc aagtgcagtc atatacgctg ttttccggca
     5221 ttccgaccta tccgaatatc ctcccggcgc ctccgccgtt gagccggact cctgatattt
     5281 tcctgttcgc caacgactac gtccagccgc tgacgcatca gtggagtgtg aacatcgacc
     5341 ggcagcttgg gcggagttat gctctgacgt ttggttatct cggcgtgcgc ggcgagcacc
     5401 tgtcgcgcac ccgcgacatc aatctccttc cggccgtcgc cgtgcaaggt tcgtttgccg
     5461 acggcacgcc ggtaacattc ttccggcacc ccgggagggc caacccgaat ttcgggcgca
     5521 tcagcctggt ggatagcggc gcggattcca tctatcacgg cgggtttgta cagctcacaa
     5581 aacgcttttc gcacaatttt caggtgcaga cttcctacac cttctcgaaa gtcatcgacg
     5641 acgacccgga tcaaacttcg gtggtggtcg gcaccgacga cgcgaaaaac gcccaggaca
     5701 ctcttcagcc gaacctcgag cgcggccgcg ggaacgccga tatccggcac cggttcgtct
     5761 tgtccggcgt gtggaatatc gattatgcga attcgctgca aaacagcgtc ttgcgcgcct
     5821 tactccgggg atacgagctt tcgaccatca cgaccgtgca aagcggccgt ccttttactg
     5881 cgactgtggg cgccgacgta aacaacgacg gcaatacgcg gaccgaccgg ccgccctacg
     5941 ttggccggaa tacgatcaac gggccgaact ttgcggatgt ggatttgcgc ttttcccgcg
     6001 aaatcggcct gtatgaaagg ctgaggatgc gcttgatttt tgaagcgttc aatcttacga
     6061 atcgggtcaa tttcgatacg atcctggcca cgcagtacaa cttcaacgcg acaaccaggg
     6121 tctttagccc cgcttcgaca ttcctccagc caagcacgac gattgcgccg cgcattctgc
     6181 aactggcggc gaagataacg ttttgacggc gtggtgatgg cccgctgcgc cgtgcggcaa
     6241 taagcagaac tggccgtgcg atggcggcgt ggaggaggcg cggctc
//
LOCUS       DASREK010000131        20523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_560994, whole genome shotgun sequence.
ACCESSION   DASREK010000131 DASREK010000000
VERSION     DASREK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560994"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /gene="pyrH"
                     /locus_tag="VFA54_06430"
     CDS             <1..373
                     /gene="pyrH"
                     /locus_tag="VFA54_06430"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682679.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZS50475.1"
                     /translation="RRRAIRHLEKGRLVIFAAGTGSPYFSTDTAASLRAVEINAAVIA
                     KATKVDGVYDKDPMRHSDALRYERVSYQDVLARNLAVMDSTAISMCRENRLPIVVFNM
                     NVPGNIFRMAMGEPIGTVITE"
     gene            377..979
                     /gene="frr"
                     /locus_tag="VFA54_06435"
     CDS             377..979
                     /gene="frr"
                     /locus_tag="VFA54_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZS50476.1"
                     /translation="MKEQHSSVATPSLGTVKEIEQQAKTRMEKCKADLHHALATIRTG
                     RASLSLLDHVRVDYYGTPTPLNQLATLSIPEPTMVTVQPWDVSQIGAIEKAIRASDLG
                     LNPANDGKIVRVPIPPLTEERRKELVKHLHHVLEEHRVAIRNIRRDANENIKKLLKDK
                     KITEDEDKRAHDEIQKLTNRSIEEIDQIGKAKEKEILEIR"
     gene            complement(1405..1756)
                     /gene="ssrA"
                     /locus_tag="VFA54_06440"
     tmRNA           complement(1405..1756)
                     /gene="ssrA"
                     /locus_tag="VFA54_06440"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1942..2736
                     /locus_tag="VFA54_06445"
     CDS             1942..2736
                     /locus_tag="VFA54_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZS50477.1"
                     /translation="MHAGLKLKQIREELGLRYREVEEASVMIAQRNHNPEFIISLSRL
                     SDIENKGTLPTLYRLYTLCAIYRLDVAEVLHWYGVDLDRLSQDAALINPSRTHPIGFR
                     TNGYGTVTLPLKLDPGIDLRRTTYLSRMIQEWGKMPLALLETLKVKEHRYAYIGTDDY
                     MMYPMLQPGSLVQIDESRREIQTFGWNNEYERPVYFFEKRDGYACSWCSLSGDRLILQ
                     PHTTSPCAPEMLLYPQEIEVIGQVVAVAMRLEGAKKLKARSAAALK"
     gene            complement(2678..3133)
                     /locus_tag="VFA54_06450"
     CDS             complement(2678..3133)
                     /locus_tag="VFA54_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50478.1"
                     /translation="MPETLPNQSGEKAIRDLWNRTLSQVPTAFGRIAYLASLRNPNTG
                     LYEHFGLAQIYSEEQADRAIRLSHAEAFGTWLNFSLAQQKADLDEYLNELDPDRQRVL
                     DTWTSLATYRNLIPMDASEAERQLFVSDLELILELLRSELSTSSRLPSA"
     gene            3547..4344
                     /locus_tag="VFA54_06455"
     CDS             3547..4344
                     /locus_tag="VFA54_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50479.1"
                     /translation="MDNAHLRDLLWYSGIAIDIVVAGRLAQLRLMGRYPVLFAYQCVA
                     SVRGLVLVVLLHGSRRLLGFYGPVYFASQLVVWILYFLLIIELYSRMVEEFSGIRRLG
                     NLVLYSGLGIVAAACSILMLSDHQAGFDYYPLVSYLALQLRSVFLSLSALTLLLLLFV
                     AHYRIRIRRNVWILYAFFGTYFVTSAAMFTMRRYFGAAFDPVRNFVGSLSYLCCLSGI
                     AWFLSKAGEAESRPISVFGSKGSQELDAALSLQLQSFNQVLVKVLKQ"
     gene            4341..4892
                     /locus_tag="VFA54_06460"
     CDS             4341..4892
                     /locus_tag="VFA54_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50480.1"
                     /translation="MTILLLLLGFIAAAAGGTGIALWRLYRTGPEAQSIPALVSEISN
                     PRRSALEVYQPMSRLFAEDDFALVGRLKPDLLKRLRRQRQRVLRLYLREIRIGFGRLH
                     TLCRILAPRSHDPNFAARITQQALTFYGLFLILEFRCALGWFLHVRVDTVDLVGALAQ
                     LRQAAQSVLADMNSGTALAGSPA"
     gene            5339..6151
                     /locus_tag="VFA54_06465"
     CDS             5339..6151
                     /locus_tag="VFA54_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50481.1"
                     /translation="MTRRMTLFGVLAAAALWTLPAQAQVAPTSSCTGPQFFKGTYATV
                     DFSGQITTAISAGTTTGGTTSGGTTTGGTTTGGTTTGGTTTGGTTTGGATTAITGPFG
                     SISVLTADGAGKITSGAAVASVNGVNVVQNFTGTYTIGTNCIGTMLLTFTAATATVAP
                     GQTTTGTTPNNPFTTGTTNPFITVGTTTGGTTTGGTTTGGSTGGTTTGGTTGGTTTGG
                     TTTSGPTISFGQGIINFVLVGDGTQFFYAQANQGVVVTGTGVRQSITGAPLP"
     gene            6307..7635
                     /gene="ffh"
                     /locus_tag="VFA54_06470"
     CDS             6307..7635
                     /gene="ffh"
                     /locus_tag="VFA54_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZS50482.1"
                     /translation="MFESLSEKLQRVFKNLRGEGKLTEANIQEALREIRLALLEADVN
                     FKVVKQLLDGIKQKSLGQEVLTAFSPTEQVVQIVRDELVRVLGSHESKLRFANEPPTV
                     MIIAGLQGSGKTTSTGKLALWLSRNGRRPLMVSVDVYRPAARRQLSVIGESVKLPVFE
                     GPPDASPLELAQAARREAVETGRDVVLVDTAGRLHIDDALMQELEALRGALRPTEILY
                     VADAMTGQDAVRSAEEFHKRLGITGVILTKMDGDARGGAALSIRFVTGQPVKLVGVGE
                     KFDALEPFYPDRIASRILGMGDVLSLIEKVEQSIDQKKALELQQKIVDNEFSLQDFRD
                     QLRHLRKLGPLESILSMLPQVGPFKALQGAQVDPKELTRLEAIINSMTIKERLNHQII
                     NGSRRRRIARGSGTTVQEVNQLLKQYAEVRKMMKSFTGGMLKKRLRTMMR"
     gene            7754..7993
                     /gene="rpsP"
                     /locus_tag="VFA54_06475"
     CDS             7754..7993
                     /gene="rpsP"
                     /locus_tag="VFA54_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZS50483.1"
                     /translation="MLSIRLARTGAKKKPQYRVVVIEKQRARNGRFLEILGHYNPTTH
                     PIVLKLNQERVQYWISKGAQPSPTVHRLMSHGAQA"
     gene            8203..8433
                     /locus_tag="VFA54_06480"
     CDS             8203..8433
                     /locus_tag="VFA54_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZS50484.1"
                     /translation="MKELIEQIAKALVDNPEEVSVRAVEGQQVTVLELRVAPGDLGKV
                     IGKQGRTARSIRTILGAAGMKLKKRFTLEILE"
     gene            8433..8966
                     /gene="rimM"
                     /locus_tag="VFA54_06485"
     CDS             8433..8966
                     /gene="rimM"
                     /locus_tag="VFA54_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZS50485.1"
                     /translation="MAGSPNDEEFVALARLMRAHGNRGELIAELESDDNRIFERFSEV
                     YLWDGSLRRQRARVERSWPHKGRLVVKFEGVDSIEQAQALAGLVVQIPAALRPPAPEG
                     RFYVTDLIGCRVLEKSSGRFLGEVQGLLETGGVPLLEVKSGEREILIPLARSICLEVN
                     PSQRLIRVDLPEGLDEL"
     gene            8966..9745
                     /gene="trmD"
                     /locus_tag="VFA54_06490"
     CDS             8966..9745
                     /gene="trmD"
                     /locus_tag="VFA54_06490"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZS50486.1"
                     /translation="MQFHLVTIFPDFFKGPLDYGVVARARQSGAIGVRIWDLRDFTSD
                     RRRTVDDRPFGGGEGMLLKVEPIFLALEKIKGETACSGGATPHVVLLSAQGRPFDQAA
                     ARRLARSEQIVLVCGRYEGVDERVAEHLADEELSVGDFVLSGGELAAAIILDAVARLA
                     PGVLGNVESARAESFEPIEASEDGVGLLDSPQYTRPADFRGWSVPEILLSGRHEDVRR
                     WRRRAALAKTLRNRPDLLEHAQLSAEDRATLEQLKGNGVLS"
     gene            9745..10089
                     /gene="rplS"
                     /locus_tag="VFA54_06495"
     CDS             9745..10089
                     /gene="rplS"
                     /locus_tag="VFA54_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZS50487.1"
                     /translation="MDRSLQTIYDRNKRTDIPEIHPGDTIRVYVKIKEGDKERLQAFE
                     GTLIARKNQGLGETITVRKVSFGQGVERIFPLHSKVIDHIDRIRTGRVRRAKLYYLRG
                     LKGKAARLRERE"
     gene            10115..10780
                     /locus_tag="VFA54_06500"
     CDS             10115..10780
                     /locus_tag="VFA54_06500"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZS50488.1"
                     /translation="MRCTRQLEARAWRQGYRFVAGVDEVGRGALFGPVVAAAVILDPA
                     RPIRGLNDSKQLLPEQREELAALIRQRALAVAVKAVDAARIDQVNIYQASRLAMRAAV
                     EALDPQPDFLLTDAVRLDARQPQRPLIKGDCRSASIAAASIVAKVDRDHWMRTWDGVF
                     PQYGLAQNKGYATPEHLAALETYGPSPLHRLSFLPVAAAARIPVLAVKPPDCFYERGL
                     FDG"
     gene            10773..11066
                     /locus_tag="VFA54_06505"
     CDS             10773..11066
                     /locus_tag="VFA54_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50489.1"
                     /translation="MASPSSLLAENSGDSQQRPLKLWRRIQTIVFILFCLEMGVVLVL
                     FPWSSMWDRNYFFALAPQFGRWFASSYLRGAISGVGLVNVWIAVSEAWRLRSA"
     gene            11369..12262
                     /locus_tag="VFA54_06510"
     CDS             11369..12262
                     /locus_tag="VFA54_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50490.1"
                     /translation="MLYKSGVACLWVFLLPQVLSADDESHGLIFYERLLASSNTFGNV
                     FRLDTTAGYSFNRHVSIDGGLPVFFVRPSGTATALAGTTSATGIGNAYLELRFAAPNS
                     LINYSTVLTGTAPTGDKDSGLSTGRATADWTNHFDRSFGPLTPFANIGFANTISDTPL
                     FVRPYTTLGFVTHLEGGGAYRVARFLEAGAAAYAIEPAGQQTVVSELVPKTAPARARA
                     ANNDGRGGRQGAFESAHITVGSADLARDNGFSFWGAVHPTRTLSLQLAFTRSAHYAFN
                     TISFGIGVNLGSVLRNMRSYD"
     gene            12335..13198
                     /locus_tag="VFA54_06515"
     CDS             12335..13198
                     /locus_tag="VFA54_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50491.1"
                     /translation="MKQWFILFTLSTWIFATGASLLAQKGQGRMGAPGIGVNLPQGSG
                     KAKAGSEAAVETRSPRAAGPVIAANVDVSSRVAANPALSTVIQPLLPPGATIAAAAAG
                     FRTQGQFIAALHVSRNLGIPFEQLKSELTENHPDSLGQAIHQLRPDLSRNTVRSEVRQ
                     ARHQAEQDVDTAQLESKLSSNAALAAKAQSLLPAGTDLQAAAAGFENIREFLVAEHLA
                     RDLNIPFAQLKAQVAGTNPVPFKQAITTLRPDLSSATIKADLKTARQEAKADLEAAGE
                     IGAEQKEAGER"
     gene            complement(13209..14357)
                     /locus_tag="VFA54_06520"
     CDS             complement(13209..14357)
                     /locus_tag="VFA54_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZS50492.1"
                     /translation="MAYDWRQARNQFPVLERWTYLNTATFGPVPRCAVEAANDYLCRR
                     NELACLDFLDWFDDADQTRAAAAALLQAAPADIAFVPNTATALSWLVQGIDWKPGDRV
                     VALEGEFPNNSYFGYALAERGADFFEAPLPGGQFSLDRFCSAIHRNTRLVLMSAINYS
                     TGLRPPLAEIGRFLQERGVLFYVDATQGLGAIRLDAPSLRAAMIAAHGYKWLLCPPGI
                     GFCYISPEVREWLRPAVFSWRSHEDWRCVDALHHGPPRLSSKAEKYEGGTQNFPGIYA
                     MRAVLEMMLQIGLSEIEQRVAHLAEKVRGVLRRTSGDMASDRLPYYDSPLIAARFPGT
                     DVSRLAVRLRDLTVVVAARHDHLRISPHFFNNEQDVARLEEALRKTGL"
     gene            14535..16715
                     /locus_tag="VFA54_06525"
     CDS             14535..16715
                     /locus_tag="VFA54_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZS50493.1"
                     /translation="MLNTCLTGCSELEESCENEKSFRILFEEAPIGYYEIDCDGIIRR
                     INKSACRLLRFDAADLIGRPLSAFASPEAVETRLERVRQRIAGEEALRPYLSEYLRRD
                     GSKIVLQTHETLIRDKNQRVVGIRSALMDATEHQLVLDALRESEERYALAVRGANDGL
                     WDWNLKTGKVYYSPRWKSMLGYDEADIGNSPEDWFSHVHPQDLGRLKIEIGLHLEGYN
                     RHFQHEYRIRHKDGTCRWVLCRGLAVRDAEGNPHRIAGSQTDITVRKLGEERLLHDAF
                     HDELTGLPNRALFIERLERAAIRAMRRENYLFAVLFLDLDGFKVINDSLSHQAGDQLL
                     VSIARRLEACLRPEDTIARLGGDEFVILLDDLKNISDATQLSERIQRELQAPFLLGRQ
                     QVFTTASIGIASSAAGYRRPEELLRNADTAMYRAKALGKARHEVFHAGLYKQAVERLR
                     IETDLRRALERGELQVHYQPIVRRESGEITTVEALVRWRQPHRGLIRCGRFMPIAEET
                     GLILPIGEWVLRTACAQNKAWQRILPSLRVAVNLSARQFKQPNLVDGIARALEDTGLD
                     AGFLDLELSESTALAGGEEALETIQKLCSMGVRISIDDFGTGYSSLKYLRRFPIDTLK
                     IDPSFVRSVDTDLDNAAIATAVINLGHSLQLNVVAEGVETEEEMEFLCSRGCDQMQGR
                     VFSEPVAAQQIVPLLERRYLPVAPRADAERTPAPARPFSFRHHS"
     gene            complement(16706..17626)
                     /locus_tag="VFA54_06530"
     CDS             complement(16706..17626)
                     /locus_tag="VFA54_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZS50494.1"
                     /translation="MSKHGALRDLAEWAAVWPVIKTLENLPLFLAQLECRVLARVLRV
                     STPRLVKVARRNLALALPGMPEPEREAIVTGVYETLARSLLSFARFPQLSRSTIGAWI
                     RYDGFEHFRRAAQKGKGVLFLTAHLGNWELSALAHALYGHPMHIVVRPLDNPHLDRLV
                     ARHRTLAGNRIVQKNESPRRILEALAANHAVGMLIDQNASPENGVFVDFFGVKACAST
                     GLVKIALRTGAPVIPGFALWNAGEGRYVLRFWPPVEITVSNDTEADVRVNTQRMHTVL
                     EQIIRQYPDQWLWIHRRWKSRPPGEPPVYE"
     gene            17717..19153
                     /locus_tag="VFA54_06535"
     CDS             17717..19153
                     /locus_tag="VFA54_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS50495.1"
                     /translation="MRLWAAAALAAYFLFFFRLTGAGLAGPDEPRYAWVGREMARSGD
                     WVTPRLWGRPWFEKPALLYWTTAAAFRAGLGEDWAPRLPVAVLSVLFLLFFFWRLKVE
                     LGNPAAGYATGVLATCAGWVAYSRVAVFDLPLSAAFGAAMLALLAWLERRQTRWLTSF
                     GAFLGVSVLAKGLVGPALAALTLLCWSARNGLRELWSPLRPAALLVFLAVASPWYVLC
                     YLRNGAPFLAEFFWRQHFSRYAAGALAHNQPLWYFAPVLAVGLLPWTPLLTAFGAKTL
                     WRERKLFFFLAWAAVTVIFFSFSRDKLPAYVLPALPAIAALAGAALARAKPMPIALSL
                     CAATLALFPAAAAILPEALDSGARAALRTAAFPARDMAAAAALAACVVLFERIGRRQA
                     AVAVVLAASVAGYIWIAQTSFRLIDQKAGARSLWRQIQGRQQDLCLDGAPRSVAYGLY
                     YYAGRIVPDCSVEPRPVHIRDNEAVLHF"
     gene            19165..20154
                     /locus_tag="VFA54_06540"
     CDS             19165..20154
                     /locus_tag="VFA54_06540"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZS50496.1"
                     /translation="MLAAMMRLVCWWLLGCVLFAADPPFSILTGKPPAPPDNPPTPAK
                     VELGKKLFFDNRLSGPGNRSCGTCHRPELMYSDGLSRAWGLAESELRRKTPQLYNVGW
                     HKRLFHDARAGSLEEQAAFPLRAEFEMDLDPNTAAERIRKDPQYQRLFAAAFPGREIT
                     WKLMAEAIASFERTLVSYDSDLDRYLAGDQAALGEAAKRGMALFTGKAGCSNCHNGPL
                     LSDQKMHYIGVQEMAGDSPQGTPYKTPSLRDIVLRGSYMHNGRYRTLDEVLAFYQGVD
                     MVRSPKGEAPPLELTSSEKSDLMAFLRSLTGRVYSVDLEEPEPVGTPPFGPHR"
BASE COUNT         4010 a         6051 c         6301 g         4161 t
ORIGIN      
        1 ccggcgccgg gcgattcgcc acctggagaa gggccggttg gtgatcttcg cggctggcac
       61 cggaagtcct tacttctcga ccgacaccgc ggccagcctg cgggcggtgg aaatcaatgc
      121 cgctgtgatc gccaaagcca ccaaggtcga tggagtgtat gataaagatc ccatgcggca
      181 ttccgacgcg ctccggtatg agcgggtctc ctatcaggat gtgctggccc gcaacctggc
      241 cgtgatggat tccacagcca tttccatgtg ccgcgaaaac cggttgccga ttgtcgtgtt
      301 taacatgaat gtgccgggca atatattccg catggctatg ggtgagccaa taggaacggt
      361 gattacggag tagatcatga aggaacaaca ctcctcggta gccacgccca gtctcggaac
      421 cgtcaaggag atcgagcagc aggcgaaaac gcggatggag aaatgcaagg cggacttgca
      481 tcacgcgttg gcgaccatcc gcacgggacg ggcctcgctg agcctgctgg atcacgttcg
      541 ggtcgactac tacggtacgc caacgccgct caatcaattg gcaacgctct ccatcccaga
      601 acccacgatg gtgactgtgc agccgtggga cgtttctcag attggcgcga tagagaaagc
      661 gattcgggcc tcggatctcg gccttaaccc ggccaacgac ggcaaaattg tgcgcgtgcc
      721 cattccgccg ctgacggagg aacgccgcaa ggagttggtc aagcatctac atcatgtgct
      781 ggaagagcat cgcgtggcga tccgcaatat ccgccgcgat gccaatgaga acatcaaaaa
      841 gcttctcaaa gacaagaaga ttaccgaaga cgaagacaag cgggcgcacg acgaaatcca
      901 gaagcttacc aaccgttcga tagaagaaat cgatcaaatc ggcaaggcca aagagaaaga
      961 gattctcgag atcagatagg tcccaggcgc ggcatccgcc ggagcaggtt cagaatgccg
     1021 ctgtgccggc gtcatgcctc gcgcatcgtc agcagaccac aggcgcggta gccgagtctc
     1081 gaagatgccg gcccgcggaa atcagggttg ctcagcatcc gccggcggtg tgacggacag
     1141 aatcggctcc gtattgggag actcgcccgc agcccgggcc tgttgccgct ccagtttccg
     1201 ttgcagccgc ttagccgcct tgtcttgctt cttttgggcg cgggcctgct ctttctgccg
     1261 cttttgaaaa gtagtccgtg atcgcgaagc caaagcctcc tccttgcact ttctgcttcc
     1321 gccagagccg gctggctgcc gcgtccccag gcgccgcttg ggacccaaca tgggccgcca
     1381 gaagggcggg aactagtgtg acattggtgg aggcggcggg agtcgaaccc gcgtccggaa
     1441 aagctcccgg caggaagact acatgcttag tcgcgcttga tagtgtcggc cggcgtatcg
     1501 aagcggacca aactacaccg ccctagcccg attagtttcg gcctgcggct ccgagcagaa
     1561 gccctcggcc tagcccgctg aaattacgcc ccttagactc cttgcgggcc cggagtccag
     1621 gaacggctgc ctaaggacta agcagcgtaa gctaactgcg tgttggcagt tattggtttc
     1681 cgatcgtttt acgggagctc ggaacccggc atgccttccc accgcgattc aatcccgtcg
     1741 aatcctttac gccccctttc tccaatagct tagcacaaga ggccgccagt acccaagagc
     1801 aggaatctgc tattatgaaa caagcgcgga aattgtgttc tgaaaacaga acaaaaatgg
     1861 atagcccggc gttgcccgcc cggaggccaa atcgatataa tcggcattct aagatttttc
     1921 attcatttgt gagcgaaaaa aatgcacgcc ggcctgaaac tcaaacaaat tcgcgaggag
     1981 ctggggctgc gctaccgcga agtggaggag gccagcgtga tgatcgcgca gcgaaaccac
     2041 aaccccgagt tcattatctc gctgagccgc ctttccgaca tcgagaataa gggcaccctg
     2101 cccactttgt atcggctgta cacgttgtgc gccatttacc ggctggatgt ggccgaggtc
     2161 ctgcactggt acggggtgga tctggaccgc ttatcgcagg acgcagccct gatcaacccg
     2221 agccggaccc acccgatcgg ctttcggacc aacggctacg ggaccgtgac gctgcccctg
     2281 aagctggatc ccggtatcga cctgcgccgc acgacctacc tcagccgcat gattcaggaa
     2341 tggggcaaaa tgcctttggc gctgctcgaa acgctcaagg tgaaagagca ccgctacgcc
     2401 tacatcggca cagacgacta catgatgtat cccatgctcc agcccggctc gctggttcag
     2461 atcgatgaaa gccgccgcga gattcagacg ttcggatgga acaacgaata cgagcggccg
     2521 gtatacttct tcgagaagcg ggatggatat gcgtgcagtt ggtgcagcct gagcggcgac
     2581 cgtttgatcc tacagcctca taccacttcg ccttgcgcgc cggagatgct gctttacccg
     2641 caggaaattg aggtgatagg ccaagtcgtg gccgtcgcta tgcgcttgga aggcgcgaag
     2701 aagttgaaag ctcgctccgc agcagctcta aaataagctc cagatcggac acaaaaagct
     2761 gcctttccgc ctcgctggcg tccataggga tgagattgcg gtaggtggcg agcgatgtcc
     2821 aggtatccag cacgcgctgg cggtcggggt ccagttcatt taaatactcg tccaaatcgg
     2881 ccttctgctg cgcgagggaa aagttaagcc aggttccgaa ggcttcggcg tggctcaacc
     2941 ggatcgcccg gtccgcctgc tcctcagagt agatctgggc caggccaaag tgttcgtaga
     3001 ggcccgtatt cgggttgcgc agggacgcca ggtaggcgat ccggccaaaa gctgtgggta
     3061 cctgggagag cgtccggttc cacagatcgc gtattgcttt ttccccactc tggttgggaa
     3121 gagtttccgg cattccagtt ccctttttct cccgccgccg cgttatacct gcgggggatt
     3181 ttaacacatt ccgcgtgcgt tttctccgcg ctgacggctg gcggccagac ccgcactagc
     3241 tgagcaagtt ccgttccata cccgaaaaat atgtctgccg ggcgagaagc cgatagccgc
     3301 tcgtactaca gtggcttacg gtaagcgggc ccaaaatttt cgagtggaag gcacaggaga
     3361 agcaaatgat taagaaagcg attcttttag cggcggtttt actgcaaatc gcgctgtttt
     3421 cagtaggacc gatggcggcc gatgacccgc ccatgccgcc ctgctggccg tgcgcaatgt
     3481 aattttgcgc aaaatttgaa aaaagaggtg ctataatcct gggacgggtt tgtaaacccg
     3541 cccgcaatgg acaatgcgca ccttcgggat ttgttgtggt atagcgggat agcgatcgat
     3601 atcgtcgtag cgggccgttt ggcccagttg agactgatgg gtcgctatcc tgtccttttt
     3661 gcctaccaat gcgttgcctc tgtacgcggg ctggtgctcg tagtccttct ccatggcagt
     3721 agaaggctgt tgggttttta cggtccggtc tattttgcca gccagcttgt agtctggatt
     3781 ttatattttc tcttaattat cgaattgtat tcccgcatgg tggaggagtt ctccgggatc
     3841 cggcgtttgg ggaatctggt tctctactca ggcctcggta ttgtagccgc cgcttgcagc
     3901 atattgatgt tgtcggatca tcaggcgggc ttcgactatt acccgctggt ttcctatctg
     3961 gccttgcaac tacgaagcgt gtttttgtcg ttgagcgcgc tgacgctcct gctgcttttg
     4021 ttcgtcgctc actaccgaat tcgaatccgg cgcaatgtct ggatcctcta tgcttttttc
     4081 ggaacctact tcgtcaccag cgccgccatg ttcacgatgc gccgatattt cggcgctgcc
     4141 tttgatccgg tccgcaattt tgtgggttct ctctcgtatc tgtgttgcct gtcggggatt
     4201 gcctggtttc tttccaaggc aggggaagcc gaatcccggc cgataagtgt ctttgggagc
     4261 aaaggaagcc aggaattgga cgctgccctg tcattgcagt tgcagagttt taaccaagtt
     4321 ttggtgaagg ttttgaaaca atgacaatac tgttactact cctcggcttt atcgcggcgg
     4381 ccgccggggg tactggcatc gcgttgtggc ggctttaccg cacggggccg gaagcgcagt
     4441 ctatcccggc tttggtctct gaaatttcaa atcccaggcg gtcagccctg gaagtgtatc
     4501 agccgatgag ccggctgttt gctgaagacg attttgctct ggttggccgg ctcaagcctg
     4561 acttgctgaa gcggctgcgc cgccagcgcc agcgagtgct ccggttgtat ctgcgggaga
     4621 tacgaatcgg cttcgggcgt ctgcacactc tttgccgcat tctggcgccg agaagccacg
     4681 acccgaattt cgccgctcgc atcacgcaac aggcgctgac gttctacggc ctgttcttga
     4741 ttctcgagtt tcgatgcgcg ctcggctggt tcctgcacgt gcgggtcgat acggtcgatc
     4801 tcgtcggcgc cctcgcccaa ctgcgccagg cggcgcagag tgtgctggcg gacatgaact
     4861 ccgggacggc cctggccggc agcccggcct gacctggtga gccacccggt cggccttcac
     4921 cagcgcgggg gccgggcccc ttgaagcgtt ttcgaagccc gaagagaagc cttttcggag
     4981 cccgcgtacc cttgcggacc tggtggcgtc tgccggaagc ttcagccgga agcagtgcgc
     5041 tttttgttcc ttactggttc cgttggacgg gaccccctat caatttttcg cagttctgat
     5101 ttccactttc gcaaaggtcg ccgagtaaaa ccgcgcacga gatcgggatt ttgccggcgt
     5161 tttttttact ttggctccgt tctcgctaaa gaagccgaga cggaagcggc atgtccatcc
     5221 ttccgatgct gtcgatcgag ctcggcggat cagccaggcc ggctttccga gagtctcgct
     5281 acagcaggcg aagtgaaaac ctccgcatcc taatcggcaa atcgaaagga gctgaagaat
     5341 gacaaggcga atgacgctgt tcggggtctt ggcggcggcg gctctatgga cgctcccagc
     5401 gcaagctcag gtggccccta cgagcagttg cacagggccc caatttttca aaggcaccta
     5461 cgcaaccgtg gatttctccg gacaaattac aaccgcgata agcgccggaa caacaacggg
     5521 cggtacaaca tcagggggga cgacaacggg cgggacaaca accggaggaa caaccactgg
     5581 aggtacaaca acaggcggca cgacgaccgg aggagcgact accgcgatta caggcccctt
     5641 cgggtcgatt agcgtactca cagccgatgg cgcgggcaaa atcaccagcg gtgcggcggt
     5701 tgctagtgtg aacggcgtga atgtagtcca aaacttcacg ggtacctaca cgattggcac
     5761 gaactgcatc gggaccatgc ttcttacctt caccgctgcc accgcaaccg ttgctccggg
     5821 acagaccacg acgggaacga cgccgaacaa ccccttcacc accggcacaa ccaatccctt
     5881 catcaccgtt gggacgacca ctggcggcac aaccacagga ggtacgacta cgggcggctc
     5941 gactggggga acgacgaccg gtggaacgac cggcggcacg acgactggcg gcacaacaac
     6001 cagcggcccc actatcagct tcgggcaagg gatcatcaat ttcgtgctgg tcggcgatgg
     6061 cactcagttc ttctacgcgc aagcgaatca gggagtagtg gtgacgggaa cgggcgtgcg
     6121 gcaatccatc accggcgccc ctcttcctta agcgcaataa acgctggctg tgccggcgct
     6181 ttcgggcgcc ggcctcttta gcgttttgca agcggggcgg cgtttgagaa acgtccccgc
     6241 gtccaggatc gaactggccc cggggccggg gacgctcttc ttctggccgc ctgctagaat
     6301 agtcgtttgt ttgaaagtct cagcgaaaaa ctacagcgtg tcttcaaaaa cctgcgcggc
     6361 gaaggcaagc tgaccgaggc gaacatccag gaagccttgc gggaaatccg gctcgcgctc
     6421 ctggaagccg acgtcaactt caaggttgtc aagcaacttc tggacggcat caagcaaaag
     6481 tcccttggcc aggaggtgct cactgccttt tcgcccaccg agcaggtcgt gcagatcgtg
     6541 cgcgatgaac tggtgcgcgt gctcggcagc cacgagtcga aactgcggtt cgccaacgag
     6601 ccgccgaccg tgatgataat cgccggcctg caaggctctg ggaagacgac ttcgaccggc
     6661 aagctcgccc tatggctttc ccgcaatggg cgccgcccgc tgatggtttc cgtggatgtg
     6721 tatcggccgg cggcgcggcg ccagttgagc gtaattggcg agtcagtcaa gctgccagtc
     6781 ttcgagggcc cgccggacgc ctcgccgctc gaactggctc aggcggcccg cagagaggcg
     6841 gtggaaacag gccgcgacgt agtgctggtc gacactgccg ggcgcctgca tattgatgat
     6901 gccttgatgc aagagctcga ggccctgcgg ggggccctgc gccctacgga aatcctttac
     6961 gtcgccgacg cgatgaccgg acaggatgcg gtccgttcgg ccgaggagtt tcataagcgt
     7021 ctcggaatca ctggcgtcat cctgaccaaa atggacggag acgcccgcgg cggcgcggcg
     7081 ctatccatcc gattcgtcac cggccagcct gtcaagcttg tgggggttgg agaaaagttc
     7141 gacgcgctcg agcctttcta tcccgaccgc atcgcctccc gcattctcgg catgggcgac
     7201 gtcctttccc tgatcgaaaa agtagagcag agcatcgatc agaagaaggc tcttgaactc
     7261 cagcagaaaa tcgttgacaa cgaattttcg ttgcaggatt tccgggatca gcttcgtcac
     7321 ttgcgaaagc taggcccgct cgaatccatc ctgtcgatgc tgccccaggt gggtcccttt
     7381 aaggcgctgc aaggcgccca ggtggatccg aaggaattga cgcgtctgga ggcgatcatc
     7441 aactcgatga cgatcaagga gagattgaac caccagatca tcaacggcag ccggcgccgc
     7501 cggatcgcgc gggggtctgg aaccacagtc caggaagtca atcagcttct gaagcagtac
     7561 gccgaggtcc gcaaaatgat gaagtccttt actggcggca tgctgaagaa gcgtctccgc
     7621 acgatgatgc gctaggtaga gccggctggc cacccaggag ccggccgccg cgcgcaggcg
     7681 tcgccgtttt ccaaaaccag cgggaaactt ggtaaactgg gtatcccata tgaacttaaa
     7741 ttgaggagca agaatgcttt cgattcgctt ggctcgcacc ggagccaaga aaaagccaca
     7801 gtaccgggtc gtagtgattg agaagcagcg ggccaggaac ggccgttttc ttgagattct
     7861 cggccactac aaccccacca cccatccgat cgtcctgaaa ctcaaccagg agcgcgtcca
     7921 gtattggatc agcaagggcg cccagccctc gcccacggtt caccggttga tgtcgcacgg
     7981 cgcacaagcc tgaccgtagc ttatagcgca gagaaaggat ggacgccggg gccgtcatat
     8041 gccgtccggg aggcctctgt tataatcggc ctaggagcag atgactttgc tcttataaaa
     8101 aagaaagagt ttggggtact gctagagagg cacttctgag cgcacgttcc agttgcagcg
     8161 aagaggacgg ggagaagctg ctgttcgtcc aggaggcgcg agatgaaaga gttgatcgag
     8221 cagattgcca aagctctggt cgataacccc gaagaggttt cagttcgagc ggtcgaaggc
     8281 cagcaagtca cggtgctgga gctccgcgta gctccgggag atctgggcaa ggtcattggc
     8341 aaacaggggc gcacggcacg atctatacgg accatcctgg gggccgccgg catgaagttg
     8401 aaaaagcgtt ttacgctgga aattctcgag tagtggcggg cagcccgaat gacgaagagt
     8461 ttgttgcctt ggcccgcctg atgcgagcgc acggaaaccg gggagaactg atcgcagagc
     8521 tggaaagcga cgacaatcgc atcttcgagc gtttttctga ggtttacctc tgggacggct
     8581 ctctacggcg gcaaagagcc cgtgttgaaa ggagctggcc ccacaagggc cggctcgtcg
     8641 tcaagtttga gggcgtcgac agcatcgagc aggcccaggc tttggccgga ttggtcgtgc
     8701 agattccggc ggcccttcgc ccgccagctc cggaagggcg tttttacgtc acggatttaa
     8761 tcggctgccg cgtgctggag aaaagttcgg gccggtttct gggagaagtg caagggctgt
     8821 tggagaccgg cggcgttccg ctgcttgaag tgaaaagcgg ggaacgggaa attttgatcc
     8881 cgctggcgcg gtcgatttgc ttggaggtga atccctcgca gcgcttgatt cgcgtggatc
     8941 tgcccgaggg attggatgaa ttgtagtgca gttccatttg gtcaccatct ttccggattt
     9001 tttcaagggg ccgctcgatt acggagtggt cgcgcgcgcg cgccaaagcg gagccattgg
     9061 cgttcgtatt tgggacctgc gggatttcac ctccgaccgg cgccggactg tggatgacag
     9121 gcctttcggc ggcggcgagg gaatgctgct gaaggtggag ccgatctttc tggctctgga
     9181 aaaaatcaag ggtgaaacgg cctgttccgg cggcgcgact ccgcatgttg tgctgctgtc
     9241 ggctcaggga cggcctttcg atcaagccgc ggcccgccgg cttgcccggt cggaacagat
     9301 cgttttggtg tgcgggcgct atgaaggggt ggacgaacgg gttgcagagc atctggccga
     9361 tgaagaattg tcggttggcg atttcgtcct gagcgggggc gaactggctg ccgcaatcat
     9421 cttagacgcc gtcgcccgat tggcgccggg cgttctggga aacgtggaaa gcgcgagggc
     9481 cgagtcgttt gaacccatcg aggcttccga ggacggcgtg ggtttgctgg attcccccca
     9541 gtacacgcgg cctgcggatt tccgcggatg gtcagtgccg gaaattctct tgagcgggcg
     9601 ccacgaagac gtgcggcgct ggcggcggcg cgccgcattg gcgaaaacgc tgcggaatcg
     9661 ccccgatttg ttggagcatg cgcagctcag cgcagaagat cgcgctaccc tggaacagtt
     9721 gaaaggcaat ggagtgttgt catgatcgac cgatctttac aaactattta cgaccgcaac
     9781 aagcggactg acattccgga aatccatccg ggcgatacga ttcgcgtata cgtcaaaatc
     9841 aaggaaggcg acaaggagcg gcttcaggcg tttgaaggga ctttgatcgc ccgcaagaat
     9901 caaggtcttg gagaaacgat cactgtccgg aaagtcagct ttggccaagg cgtcgagcgc
     9961 attttcccgc tgcactccaa agtgatcgac cacatcgaca gaattcgcac cggccgcgtt
    10021 cgccgggcga agctatacta tttgcgaggg ctgaaaggca aagccgcgcg cttgcgggaa
    10081 agagagtagg cggctgccgg ctctctgcgc ggcgatgcgt tgcactcgcc aactcgaagc
    10141 gcgggcctgg cgtcaaggct atcggtttgt cgcgggcgtc gatgaggtcg ggcgtggcgc
    10201 gctgttcggg cccgtggtgg cggcggcggt aattctcgat ccggcccgac ctatacgggg
    10261 attgaatgac agcaagcagt tgctgccgga gcagcgcgaa gaactggccg cgttgatccg
    10321 gcaaagggcg ctggcggtcg cggtcaaggc tgtggatgcg gctcgcatcg accaggtgaa
    10381 tatttaccag gcgtcccgtc tcgccatgcg cgccgcggtg gaggcgctgg atccgcagcc
    10441 cgatttcctg ctcaccgacg cagtgcgcct agatgcccgg cagccccaac ggcccttgat
    10501 taaaggcgat tgccgctccg catcgatcgc cgccgcctcg attgtcgcga aggtcgaccg
    10561 agaccactgg atgcgcacct gggacggtgt ctttcctcaa tacgggctgg ctcaaaacaa
    10621 gggctacgcc acgccggagc acctagctgc cctggagacc tacggccctt ccccactgca
    10681 ccgcctgagc tttcttcccg tcgccgcggc ggcgcgcatt cccgtcctcg ccgtgaagcc
    10741 gccggattgt ttctacgaga ggggcttatt tgatggctag tcctagcagc ctgctggcgg
    10801 aaaattcggg agactcgcag cagcgtcctc tcaagctctg gcggcgtatt caaaccattg
    10861 tctttatcct gttttgcctg gaaatgggag tcgtgctcgt gctgtttccg tggtcctcta
    10921 tgtgggaccg gaattacttc tttgccctgg cgccccaatt cgggagatgg tttgcaagca
    10981 gctacctgcg cggcgcgatc agcggagtag ggctagtgaa cgtttggatc gccgtttccg
    11041 aggcgtggcg gctacgctcc gcctgagcct atatcacttc cttttcttcc aactcatatt
    11101 tccacagcgg acttctcccg gcacaaaaaa gcggggaggg gacaagcgcc gaaaaacttg
    11161 cgttttcccc tttttcatcc gcccgccctt ggccattgcg caagtctgcc tctgctgaag
    11221 cactggcttt gtattttttg cagcctcagg ccggtaagag cttccgttgg cgcctggctc
    11281 tctaggagtc ccccgaacaa tcctctcaga atcagcgact tacaggaagg tgtggctgga
    11341 atatcgattg cagcccgtag ctggtgatat gttatacaaa tcgggggttg cgtgcctctg
    11401 ggtgtttctt ttgccgcagg tgctctcagc ggatgacgaa tcccacgggc tgatttttta
    11461 cgagcgcttg ctcgccagtt cgaacacctt tggaaatgtc ttccggttgg ataccacagc
    11521 cggttatagc ttcaaccgcc acgtttcgat cgatggcggc cttccggttt tctttgttcg
    11581 gccctcggga acggcgacag ctttagccgg cacgacctcc gctacgggaa tcggcaacgc
    11641 gtatctggag cttcgattcg cagcccccaa ttccctgatc aactactcta ccgttttgac
    11701 cggaaccgcg ccgaccggtg acaaagactc aggcctgagc accggccggg cgaccgctga
    11761 ctggaccaat cacttcgaca gatcgttcgg gccgctgact ccgttcgcca atatcggatt
    11821 tgcaaacacg atctctgaca cgccgctctt cgtccggccg tacactacgc tcggctttgt
    11881 cactcatctg gaaggcggcg gagcttatcg cgtagcacgg tttctggaag ccggcgcagc
    11941 agcgtatgcg attgaaccgg cgggacagca gactgtcgtc agtgaactgg tcccgaagac
    12001 cgcgccagcc cgagctcggg ccgccaacaa tgacggccgg ggcggccggc agggcgcgtt
    12061 cgaaagcgcg catatcaccg tcggctccgc cgacttggcg cgcgacaacg gtttttcctt
    12121 ctggggcgcc gttcatccaa ccaggactct cagcctacaa ctagccttca cacggagcgc
    12181 ccactacgcg ttcaacacga tttcctttgg catcggagtg aatctggggt cggttttgag
    12241 gaacatgcgg tcctatgact gagtgctatt tcggcaaacc cgggggagcg aagcgtctta
    12301 cctcgctccc cccgcaagaa aggagaagtc gccaatgaag caatggttca ttctctttac
    12361 tctatctacc tggattttcg cgacaggcgc aagcctgctg gcccaaaaag ggcaagggag
    12421 gatgggagcg ccgggaatcg gcgtgaacct tccccagggg agtgggaaag cgaaagccgg
    12481 cagtgaggcg gcggtcgaga cgcggtcccc gcgggcggcg gggcctgtga ttgccgcgaa
    12541 cgtagatgtc agcagccggg tggcagccaa ccccgccctc tccactgtca ttcagccttt
    12601 gcttccgccg ggcgcaacca tcgcggccgc ggccgccggt tttcggactc aaggacaatt
    12661 catcgccgct ctgcatgtgt cgcggaacct gggcatcccg tttgagcagt tgaaatcgga
    12721 gcttacggag aaccatcccg actcgctcgg gcaagcgatt caccaactcc ggccggacct
    12781 cagcaggaac acggtccgga gcgaggtccg ccaggcgcgg catcaggccg agcaagatgt
    12841 ggacacggcg cagttggaaa gcaagctctc ttccaacgca gctctggccg cgaaggcgca
    12901 gtcgcttctt ccagccggta cagatctgca agccgccgcg gcgggattcg agaacatccg
    12961 ggagtttctt gtggctgaac atctggcccg cgacttaaac atcccgtttg cgcagctcaa
    13021 ggcccaggta gcgggcacga atccggtccc gttcaagcag gccatcacca ctctgaggcc
    13081 ggatctcagc tcagcgacca tcaaagccga cctgaagaca gcccgccagg aagccaaagc
    13141 ggacctggaa gccgcgggag agataggcgc agagcagaag gaggctggtg aacgttagcc
    13201 gcatcggctc acaggccggt ttttcgcaga gcctcctcca ggcgcgccac gtcctgctcg
    13261 ttattgaaaa agtgcggcga aatccgcaaa tggtcgtggc gcgccgcgac cacaacggtc
    13321 agatcccgca gtcggacggc taagcggctc acgtcggtcc cagggaatcg cgcggcgatc
    13381 agcggagaat cgtaataagg gagacgatcg gacgccatgt cgccgctggt ccggcgcaaa
    13441 acgccgcgga ccttctcggc gaggtgcgcc acccgctgct caatttcgct cagcccgatt
    13501 tgcagcatca tctccagtac ggcccgcatg gcgtagatgc cggggaaatt ctgggttcct
    13561 ccctcgtatt tctcggcctt tgaggaaagc cttggcgggc cgtggtggag agcatccacg
    13621 catctccagt cctcgtggct gcgccaactg aagactgctg gccgaagcca ttcccgcact
    13681 tcggggctga tatagcaaaa cccgatgcca ggcgggcaca gcagccattt gtaaccatga
    13741 gccgcgatca tggcggctcg tagggaggga gcatcgagcc ggatggctcc gagcccctgc
    13801 gtggcatcca cgtaaaaaag gacgcctctt tcctgaagga agcggccgat ttcggctaaa
    13861 gggggccgca ggcctgtcga gtaattgatc gcgctcatca gcacgagcct ggtgttgcgg
    13921 tggatcgccg agcaaaagcg gtcgagggag aactggccgc cgggaagagg cgcttcgaag
    13981 aaatcggctc cccgctcggc cagcgcatat ccgaagtagg agttattggg gaactcgcct
    14041 tcgagcgcca cgactcgatc gcccggcttc caatcgattc cctgcacgag ccagctcagg
    14101 gccgtggcgg tgttggggac gaatgctata tccgccggcg ccgcctgcaa cagggctgcg
    14161 gctgccgccc gagtctggtc ggcgtcgtca aaccagtcga gaaaatccag gcaggcgagc
    14221 tcgttgcggc ggcacagata gtcgttggcg gcttccaccg cgcaccgggg caccggacca
    14281 aatgtggctg tgttcaagta ggtccagcgc tccagaacgg gaaattgatt gcgcgcctgc
    14341 cgccagtcat aggccatggc tgcattgtaa acatgagccg gaatccctgc atctacatac
    14401 tgttaatcac acgctgataa ggtccgtggc cgaaggaata gttcctcggt accagggtga
    14461 aaatatgaac gacgtactat tgtccgccct tatggccggg tcgtagcctt gtaggacggg
    14521 ccgctcttcc gcacatgctg aatacgtgtc ttactggatg ttcggagctg gaagaatcgt
    14581 gcgaaaacga gaaatccttt cgcattttgt tcgaagaagc tccgatcggc tactacgaga
    14641 tcgactgcga cggcattatc cgccggatca ataagtcagc ctgccggctg ctgagattcg
    14701 acgcggccga cctcatcggc cggcctctct cggcattcgc gtcgccggaa gcggtagaaa
    14761 cgcgcctcga acgcgtgcgc cagaggatcg ccggagaaga agcgctgaga ccctacctct
    14821 ccgaatacct gcgccgggac ggctccaaaa tcgttttaca aacgcatgag acgctgattc
    14881 gggacaagaa ccagagggtg gtcgggatcc gatcggctct gatggatgcg acggagcacc
    14941 agcttgttct ggacgctctg cgggaaagcg aagagcgcta tgctctggcc gtgcgcggcg
    15001 ccaacgacgg cctttgggac tggaacttga agacgggaaa agtttactac tcgcctcgct
    15061 ggaaatccat gctcggctac gacgaagccg acatcggcaa cagtccggag gactggttca
    15121 gccacgtgca cccgcaggat ttgggccgcc tgaagatcga aatcggcctc cacctggagg
    15181 gatataaccg ccattttcag catgagtacc ggatccggca caaggacggc acttgccgct
    15241 gggtgctgtg ccggggattg gccgtgcggg acgcggaagg gaacccccat cgcatagccg
    15301 ggtcgcagac tgacattacc gtgcggaagc tgggcgagga gcggctgctg catgacgcgt
    15361 ttcacgatga gctgaccggc ctgcccaatc gcgccctttt catcgaaagg ctggagcgcg
    15421 ccgcgatccg ggccatgcgc cgcgaaaact atctgtttgc cgtgctgttc ttggatctcg
    15481 acggtttcaa ggtcatcaac gacagtctca gccaccaggc cggggaccag cttctggtaa
    15541 gcatcgccag gcggctggaa gcctgcttgc gcccggagga caccattgcg cgtctgggcg
    15601 gagacgaatt cgttattctg ctcgacgatc tgaagaacat cagcgacgcc acgcagcttt
    15661 ccgagcgcat tcagagagaa ttgcaggcgc cgttcctgct gggacgccag caggtgttta
    15721 cgacggcgag catcggcatc gcctcgagcg ccgccggcta ccggcgacct gaggagcttt
    15781 tgcgaaacgc ggataccgcc atgtaccggg ccaaggcttt gggaaaagcg cgtcacgaag
    15841 tattccatgc gggactgtac aagcaggcag ttgaacggct gcggatcgaa accgacctgc
    15901 gccgggcgct ggagcgcggc gagcttcagg tccattacca gccgatcgtc cggcgggaga
    15961 gcggcgagat cacgacggtc gaggcgctcg tgcgctggcg gcagccgcac cggggattga
    16021 tccgttgcgg ccggtttatg cctatcgccg aggagaccgg ccttattctt cccatcggcg
    16081 agtgggttct gcgaacggcc tgcgcgcaga acaaagcctg gcagcggatt cttccttccc
    16141 tgcgagtggc ggtgaacctt tcggcccggc aattcaagca gccgaatctt gtggacggca
    16201 tcgcccgcgc gctcgaggac accggtcttg acgcggggtt cctggattta gaactgagcg
    16261 aaagcaccgc tctggccggc ggcgaggaag ctttagaaac cattcaaaaa ctgtgttcta
    16321 tgggcgtgcg catctccatt gacgattttg ggaccggcta ctcttcgctg aaatatctgc
    16381 ggcgcttccc gatcgacacg ctcaaaatcg acccctcctt tgttcgctcc gtcgataccg
    16441 atctggataa cgccgcgatc gcgacggcgg taatcaatct gggccacagc ctccagctca
    16501 atgtcgtcgc ggagggcgtt gaaacagagg aagagatgga attcctttgc tcgcgcggct
    16561 gcgaccaaat gcaaggccgc gtgtttagcg agccggtggc ggcacagcaa atcgtgccgt
    16621 tattggaaag gcgctatctg ccggtcgcgc cgcgagcgga cgcggagcgt accccggctc
    16681 ccgctcggcc cttctccttc cggcatcatt cgtagaccgg cggctctccc ggcgggcgcg
    16741 atttccaccg gcgatgaatc caaagccatt ggtccggata ctggcggatg atctgctcga
    16801 gcacggtgtg catccgctgc gtattcaccc gaacgtccgc ttccgtatcg tttgacaccg
    16861 tgatttccac cggcggccag aagcgcagca cgtagcggcc ctcgcccgca ttccagagcg
    16921 caaagcccgg gatcaccggc gctccggtgc gcaatgcgat cttgaccagg ccggtggaag
    16981 cgcaagcctt gacgccgaaa aaatcgacga acacgccgtt ttccggcgac gcgttttggt
    17041 cgatgagcat ccccacggcg tgattggcgg cgagcgcctc taaaatgcgg cgcggcgact
    17101 cgttcttctg gacgattcga ttgcccgcca gagtccggtg gcgggccacg aggcggtcga
    17161 gatgcgggtt gtccagcggc cgcacgacaa tgtgcatggg atggccatat agcgcgtgag
    17221 ccagggcgct gagctcccag ttccccaggt gcgccgtaag gaagaggact cccttgcctt
    17281 tttgcgccgc tcgccggaag tgctcgaatc cgtcgtagcg gatccacgct ccgattgtgc
    17341 tgcggctcaa ctgcgggaac cgggcaaagg aaagcaacga tcgggcgagc gtctcataca
    17401 caccagtcac gatagcttcg cgttccggct caggcatccc gggaagcgcc agcgcaagat
    17461 tgcgccgcgc caccttgacc agacgaggcg tcgaaactcg cagaactcgg gccaacaccc
    17521 ggcattccag ttgcgccaga aacagcggca ggttttcgag ggtttttatc acgggccata
    17581 cggcagccca ttcagccagg tcgcgcagcg cgccgtgctt ggacattccc aaaaacaata
    17641 taccttgaag cggccggcca cgaagggaac ggcagccgtt ccagcattct cgtgacgcag
    17701 ccgctacact gggggagtgc ggctctgggc ggctgcggcg ctcgcagcgt attttttgtt
    17761 tttctttcgc ctcaccggcg ctggcttggc cggacccgat gagcctcgct atgcttgggt
    17821 tggccgtgaa atggctcgct cgggagattg ggtgacgccg cggttatggg gccgtccttg
    17881 gtttgaaaaa cccgcactcc tttactggac aaccgccgcc gcgttccggg ccgggctcgg
    17941 cgaggactgg gcgccgcggc tgccggttgc cgtcctcagc gtgctgtttc ttctcttttt
    18001 tttctggcgc ctgaaagtgg agttgggaaa tcccgcagcc ggttatgcca caggcgtcct
    18061 ggcgacctgc gccggctggg tggcttacag ccgcgtggcc gtttttgatt tgcctctcag
    18121 cgccgcattt ggcgcggcca tgctggcgct gcttgcctgg ttggagcgcc gccagacgcg
    18181 ctggctgacg tcttttggcg catttctcgg agtatcagtt ctcgccaaag ggctggtcgg
    18241 gccggcgctt gcggccttga cgctgttgtg ctggtcggcg cggaatggct tgcgggaatt
    18301 gtggagccct ttgcggcccg cggctctcct tgtctttctc gcggtcgcct cgccctggta
    18361 tgtgctctgc taccttcgca acggcgcgcc gtttctcgcc gaatttttct ggcggcagca
    18421 tttctcccgg tatgcggccg gcgcgctggc gcacaaccag ccgctgtggt atttcgcgcc
    18481 tgtattagct gtagggctgc ttccctggac gccgctgctg actgcgttcg gagcgaagac
    18541 gctttggcgc gagcgcaagc ttttcttctt tctggcatgg gcggcggtca cggtgatctt
    18601 tttctcgttc tcgcgtgaca agctgcccgc ctatgtcctt cccgccctgc ctgcgatcgc
    18661 agccctggcg ggcgcggcgc tggcgcgcgc gaagcccatg ccgatcgctc tgtccctttg
    18721 cgccgccacg cttgcattgt tccccgctgc cgccgcgatc ttgccggaag cgctcgatag
    18781 cggcgcacgc gccgctctgc ggacggctgc tttcccggcc cgcgacatgg ctgctgccgc
    18841 agcgctggcg gcgtgtgtag tgcttttcga gcgaatcggg agaagacagg cggcggtagc
    18901 tgtcgttctc gcggcgagcg tcgccgggta tatctggatt gcgcagacat cgtttcggct
    18961 gatcgatcag aaggccggcg cacgatcttt gtggcgccag atccagggcc gccagcaaga
    19021 tctttgcctc gatggcgcgc cgcgcagcgt cgcctacggg ctctattatt acgccggccg
    19081 gatcgtgccc gattgcagcg tcgaaccgcg gcctgttcac atccgggaca atgaagctgt
    19141 attgcatttt taggcgattc tgttatgctg gcggctatga tgcggctggt gtgctggtgg
    19201 ttgcttggat gtgttctttt tgcggcggat cctcctttta gcatcctcac gggcaaacct
    19261 ccggcgccgc ccgacaatcc gccgacgccc gccaaggtcg aactcggcaa gaagcttttc
    19321 ttcgacaacc ggctctccgg acccggcaac cgcagttgcg gcacctgcca ccggcctgag
    19381 ctcatgtatt ccgacggcct gtcgagggcg tgggggctgg cggaaagcga actccggcgc
    19441 aagactccgc agctctacaa tgtgggttgg cacaagcggc tctttcatga cgcgcgtgca
    19501 ggatcgctcg aagagcaggc ggcgtttccg ctgcgcgccg agttcgagat ggatctagac
    19561 cccaacacgg cggcggaacg catccgcaag gatccccagt accaaaggct ttttgccgcc
    19621 gcctttccgg ggcgcgaaat cacctggaag ttgatggccg aggctattgc ctccttcgag
    19681 cgcacgctgg tttcctatga cagcgatctg gatcgctatc tggccggcga tcaggcggcg
    19741 ctcggcgaag cggccaaacg gggcatggct ttgttcacag gcaaggctgg atgctcgaat
    19801 tgtcacaacg gtcctctgct ctccgatcag aagatgcatt acatcggagt tcaagagatg
    19861 gcgggtgact cgccgcaagg cacaccatat aaaacgcctt cactccggga tatcgtcctg
    19921 cgtggctcct atatgcacaa cggccgttat cgaaccctcg acgaagtgct ggcgttctac
    19981 cagggagtcg atatggtgcg ttcgccgaag ggcgaagcgc ctccgcttga gctcacctcc
    20041 agcgaaaaat ccgacttgat ggcttttttg cgctccctga caggccgcgt ttattccgtg
    20101 gatttggagg agcccgagcc ggtggggacg cctcccttcg gaccgcaccg gtagccgcac
    20161 tcgggcctca gcggccggat ccgccatcag cgcaagataa aagacctctt ctcacaaagt
    20221 aatttggaac tcctctgtct gccggtgttg ccgccggttt ttttatccgc cagacgcgga
    20281 atgagaccgg aagagagtcc attcatggat gccgtgttgc ccctctccgt gagaacatca
    20341 ggaaatcgcg cagtagctcc ggccgctgaa tctgtggtcc gaaccgcgcc gccagcagtt
    20401 cgccttgtcc gaacggcagg ggcgagccgg cctactgtat cgccattgtg atcttgtcgc
    20461 tgctggtagc gcctccgatc gagatctgga ccggcacgct atccccggag gggacgccgg
    20521 agg
//
LOCUS       DASREK010000132         4977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1884948, whole genome shotgun sequence.
ACCESSION   DASREK010000132 DASREK010000000
VERSION     DASREK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884948"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFA54_06545"
     CDS             <1..652
                     /locus_tag="VFA54_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HZS50497.1"
                     /translation="GNRRRVLISDLSGRSNIFFKLKERGLGDKLSDAARRELLERIKQ
                     LEYDGYELEAAEGTFELLVRETLHPDLFFFELESSKIVTERKKDGRTTSTASMTVRAK
                     DGVHSATASSHGPVHAMDLCLRRCLSKLYPQIADIRLTDYKVRVLDSKRGTAARVRVL
                     VEWTDHKRSWTTVGVSENVLEASLNALVDSVRLELLRLAEQDESVEKVVEDYCWGV"
     gene            656..1222
                     /locus_tag="VFA54_06550"
     CDS             656..1222
                     /locus_tag="VFA54_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50498.1"
                     /translation="MGRALRRSQLSKDPAGDHPEPALRSLPARYAHRAALLAVFGVWV
                     SIAWPGLSLQFATLDYWRQRLGGRTPEERAAIVDYPAYSVARRVEEVVPPAACVLFLA
                     YTGPEHVNYYKTRFDYYLYPRRVRIAAGTGATAENCGYLAVFRDAPANLAEEPFRGVW
                     DEGWLHHRLAPLEKVYSGPNLDIYRARR"
     gene            1219..2679
                     /locus_tag="VFA54_06555"
     CDS             1219..2679
                     /locus_tag="VFA54_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50499.1"
                     /translation="MMICAFLFTCVAGWLLICPWLSRRPGLGLVPAIALSFAAGTAAL
                     SLDLLLLNLAHIPWTRFSVLSPWIAAAAAHALFGRRRWAPPMPRLRRPCFLEIAALAF
                     SGIVLLAWVPYERLMPLNEWDAIMLWMFKAKAFYLDGSIAPYMSRAHEFLGNPAYPLL
                     VPLYTTFLYIFSGSAADQLAKLVSPCLLASLAAGFYYFLRRYGSRPVAAIFTSMLAGV
                     YMVDVAAFRYAGYADTAVAAAILLAAGFLYAWFYEGDYADFALAVFFASIAAWTKNEG
                     QFFLAGFGALAAARLISRRIWNSSYWFTLAAFPILFVTPWVAARSVYGVKRPGELSGE
                     MMRTNIASYGPTVKAIFEHAFTPAPPDAFGSRVWLFNLAFPFCLLAVFLYRRAGLDRR
                     FWALPALACWQLFGVSLVYVTGPVNLQWMIGSSLDRVLSQIAPLALLAAALAFASYYS
                     RAEARLEIPAEDGASLRKRREKARASPKKQRGKSQA"
     gene            2676..3689
                     /locus_tag="VFA54_06560"
     CDS             2676..3689
                     /locus_tag="VFA54_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZS50500.1"
                     /translation="MSRLRTVFAAFALPFFLALPAALAQPGNVPSRRDDSARPAPRPV
                     LMVTDCGVDMDDQWALAHLALSPEFNLVGVVTTHAPTLAAPAAQTSARVARDVLDHLP
                     LRFRPLVIPGSSVPLAGRKPQPNPGVDFLLRRSRSHSPDRRLDLLLTGAATDVASALL
                     IDPGLGDRIRVIAMGFNNWPRGTDPFNVKNDVTAWQVLLESRAPVVIGDTAVTNRYLR
                     MTREKARELFYPRGAPGVYLANILISWLDDNGDLVRSITGNRNAWPVWDEVTVAYLLG
                     LTKSRVHPRPVLRDDMTFEHPASGRRAGQAAREVVWITAIDAQRLWADFTYKLDRALA
                     LHH"
     gene            3799..4260
                     /locus_tag="VFA54_06565"
     CDS             3799..4260
                     /locus_tag="VFA54_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZS50501.1"
                     /translation="MNISVKGDYALKAVFDLAYRGGAGPVKIAEIAARQKIPQKFLEL
                     ILAQLKQGGFVESRRGAEGGYLLARPPDRISVGEVLRFIEGPLSPARARGAKSRPAAR
                     RSRALEEETPLTELWEQVDKAVSDIVDRTSFADLAARWSERQSRFVANWQI"
     gene            4309..>4977
                     /locus_tag="VFA54_06570"
     CDS             4309..>4977
                     /locus_tag="VFA54_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZS50502.1"
                     /translation="MSIGHTPLIKFNRIVNGAPATVLGKVEGRNPAYSVKCRIGASMI
                     WDAEQRGILGPGKELIEPTSGNTGIALAFVAAARGYPITLTMPDTMSLERRAVLKILG
                     AKLELTPDEQGMNAAIRRAEQIVASDPQRYIMLQQFKNPANPAIHFKTTGPEIWEDTD
                     GQIDVLVAGVGTGGTITGVSRYIKHEKGKKILSVAVEPAASPVITQHLNGKKLRPGPH
                     RIQGI"
BASE COUNT          833 a         1605 c         1535 g         1004 t
ORIGIN      
        1 ggggaatcgc cgccgcgtgc tcatcagcga tctttcgggc cgcagcaaca tcttcttcaa
       61 gctgaaggaa agaggcctgg gcgacaagct cagcgacgca gccaggcgcg aattgctcga
      121 gcgaatcaag cagctggagt acgacggtta tgagctggag gcagccgaag gaaccttcga
      181 gctgcttgtg cgcgagacac tgcatcccga tctgttcttc ttcgagctgg aaagctccaa
      241 gatagtgacg gagagaaaga aggacggacg cacaacctcg accgccagca tgaccgtgcg
      301 ggccaaagac ggcgtgcact cggccaccgc cagctctcat ggccctgtac atgccatgga
      361 tctctgcctc cggcgttgcc tttccaaact gtatccccag atcgccgaca ttcgcctgac
      421 cgactacaag gttcgcgtgc tcgattccaa gcgcggcacg gccgcccggg tgcgcgtcct
      481 ggtggagtgg accgaccaca agcgcagttg gactacggtg ggcgtctcgg aaaacgtgct
      541 ggaagccagc ttgaacgccc tggtggactc ggtccggctg gagctgctgc ggctggcgga
      601 gcaggacgaa tcggtggaga aggtcgtcga agattactgc tggggggtgt gaaccgtggg
      661 ccgggccctg cgccgctccc aactcagcaa agacccggca ggagaccatc cggagcctgc
      721 actgcgctcg ctgccggctc gctacgcgca ccgcgccgct ctgcttgctg tgttcggcgt
      781 ttgggtcagt atcgcctggc ccgggctttc tctccaattc gccacgctcg attactggcg
      841 gcagcgcctg ggcggccgga ctccggagga gcgggccgcg atcgtagatt atccggcgta
      901 ttccgtcgcc aggcgggtgg aagaggtcgt cccgcccgcc gcttgcgtcc tgtttcttgc
      961 ctacaccggc ccggaacacg ttaactacta caagacgcgg ttcgactatt atctctatcc
     1021 aaggcgtgtg cgcatcgctg ccggcaccgg cgcaacggcg gaaaattgcg gctaccttgc
     1081 ggtattccgc gacgcgccgg ccaatctcgc ggaagagccc tttcgcgggg tgtgggatga
     1141 gggctggttg caccaccggc tggcgcctct ggagaaggtt tactcggggc cgaatctcga
     1201 tatctaccgc gcccgccgat gatgatatgc gctttcctct tcacttgcgt cgccgggtgg
     1261 ctcctcattt gcccctggct ttcccgccgg cccggcctgg gcctggtccc ggcgatagct
     1321 ctgagcttcg ctgccggaac ggccgccctt tcccttgatc tgctccttct gaacctggcg
     1381 catatcccct ggacccggtt ttccgtattg tcgccctgga tcgccgcggc cgccgcccat
     1441 gctttgtttg ggcgtcggcg atgggcgcct cccatgcccc gtctgcgccg gccctgcttc
     1501 ctggaaattg cggcactcgc cttctccgga atcgttctgt tggcctgggt tccctatgag
     1561 cgcttgatgc cgctcaacga atgggacgcg atcatgcttt ggatgttcaa ggctaaggcc
     1621 ttttatctgg acggcagtat cgctccctat atgagccgcg cccacgagtt cctcgggaat
     1681 ccggcgtatc ccctgctggt gcccctgtat acgacatttc tctatatctt ttccggatct
     1741 gccgccgatc agcttgcgaa gcttgtttca ccctgtttgt tggcgtcgct ggcggctgga
     1801 ttttactact tccttcggcg gtacggctcc cgccccgttg cagcgatttt cacctcgatg
     1861 ctggccggcg tgtacatggt ggacgtcgca gcctttcgct acgccggtta cgccgatacg
     1921 gccgtagcag cggccatcct gctcgccgcc ggttttcttt atgcgtggtt ttacgaggga
     1981 gactatgctg acttcgccct cgctgttttc ttcgcctcta tcgctgcctg gaccaaaaac
     2041 gaaggacagt tttttctggc tggattcggc gcgctggccg cagcgcgtct gatctctcgg
     2101 cgaatttgga attccagtta ttggttcacg ctggccgcgt tccccatttt gttcgtaacc
     2161 ccctgggtcg cggcgcgcag tgtgtacggc gtgaaacgcc cgggcgagct ctccggagag
     2221 atgatgcgaa ccaacatcgc cagctacgga cctacggtca aggccatttt cgagcacgcg
     2281 ttcacgcctg ccccgccgga cgcatttggc tctcgggtct ggctttttaa cctcgccttt
     2341 ccgttctgct tgctggctgt ctttttatac cggcgcgccg gcctcgaccg gcgcttttgg
     2401 gcgctgccgg ccctggcgtg ctggcaactg ttcggcgtga gtctggtgta tgtcaccggc
     2461 ccggtaaacc tccaatggat gattggctcc tcgctcgacc gggtgctctc gcaaatcgcg
     2521 cctctcgcct tgctggctgc ggcgctcgct ttcgcgtctt actacagtag ggcggaagcg
     2581 cggctggaaa ttccagcgga agacggggct tcgctgcgaa agcggcggga aaaggcgcgc
     2641 gcgagcccta aaaaacaacg cggcaaatcc caggcatgag ccgattacgc accgtcttcg
     2701 cggcctttgc gctcccgttt tttctggctt tgccggccgc cctggctcag cccggcaatg
     2761 tcccttccag gcgggacgac agcgcccggc cggcgcctcg cccggttctc atggtgacgg
     2821 attgcggcgt cgatatggat gaccagtggg cgctcgccca tctggcgtta tctccggaat
     2881 tcaatctggt gggagtagtc accacgcacg cccccacgct ggccgcgcct gcggcgcaga
     2941 cctcggcccg cgtcgctagg gacgttctcg accacttgcc cctgcgtttc cggccgctgg
     3001 ttattccagg ttccagcgtc ccgctcgccg ggcggaaacc gcaacccaac cccggcgtcg
     3061 attttctgct gcgccgctcg cgctcccata gcccggaccg ccgtctggac ctgttgctga
     3121 cgggggcggc gaccgatgtc gcctccgcgc tcctgatcga tccggggttg ggggaccgca
     3181 tccgggtcat cgccatggga ttcaacaatt ggccgcgggg gacggatccc ttcaatgtga
     3241 aaaacgacgt cacggcgtgg caggtgctgc tcgaatcccg cgccccggtt gttatcggcg
     3301 atacggccgt gaccaatcgt tatctgcgaa tgacccgcga gaaggcccgc gagcttttct
     3361 atccgcgcgg agctccgggg gtttatctgg cgaacatttt gatatcctgg ctcgatgaca
     3421 acggagacct cgtgcggtcg attaccggga accgcaatgc atggccggtg tgggacgagg
     3481 tgacggtcgc ttatctgctt ggcctcacca agagccgggt gcacccgcgc cccgttctgc
     3541 gggacgatat gacttttgaa catccggcgt ccggccgccg ggcgggccag gccgcccggg
     3601 aggtcgtctg gatcaccgcg atcgacgcgc agcgcctttg ggccgacttc acctacaaac
     3661 tggatcgtgc gctggcgctg caccactgat tcgttcccga ggccgcctgc tgtttcacag
     3721 cggaaagcgg ctccatttga cgcccgctgc ccacttaccg taatctcaaa gcgaagcacc
     3781 gcggatgagc aagccggcat gaacatttcg gtgaaagggg actacgcgct gaaggcggtc
     3841 ttcgatttgg cgtaccgggg cggcgcagga ccggtcaaga ttgctgaaat cgccgcccgc
     3901 cagaagatcc cgcagaaatt tttggagctg atcctggcgc agctcaaaca gggcggcttc
     3961 gtcgaatcgc gccgcggcgc cgagggcggc tatttgctcg cgcgcccccc cgaccgcatc
     4021 agcgtaggcg aggtgctgcg cttcatcgaa ggccctcttt ctccggcgcg cgcgcgcgga
     4081 gccaagagcc ggccggcggc gcgccgttcc cgggccctgg aggaggagac tcccctcacg
     4141 gaactctggg agcaagtcga taaggcggtg tcggatatcg tcgatcgcac cagcttcgcc
     4201 gacctggcgg cccggtggag cgagcgacaa agccgttttg tagccaactg gcaaatctag
     4261 ttccggtgct gcctcgaagc aatgcctaga tactatacag acaactcctt gagtatcggc
     4321 catacgccgc taatcaaatt caaccggatc gtaaacgggg ccccggccac cgtgttgggg
     4381 aaagtcgaag ggcggaaccc ggcctactcg gtcaagtgcc gcatcggcgc ctcgatgatc
     4441 tgggacgccg aacagcgcgg catcctcggt ccgggaaaag agctgatcga gccgaccagc
     4501 ggcaacaccg ggatcgccct ggccttcgtg gcggccgctc ggggctatcc gatcacgctg
     4561 accatgcccg acaccatgag cctggagcgc cgggcggtgc tgaagatcct cggggcgaag
     4621 ctggaactaa cgcctgatga gcagggcatg aatgccgcca tccgccgggc cgagcagatc
     4681 gtggccagcg atccccaacg ctacatcatg ctgcaacagt tcaaaaaccc tgccaatccc
     4741 gccattcatt tcaaaaccac tggtccggaa atctgggagg ataccgacgg tcaaatcgac
     4801 gtgctggtgg cgggcgtcgg cacgggcggc acgattaccg gcgtttcccg ttacatcaag
     4861 cacgagaaag gcaagaagat tctgtccgtg gctgtcgaac cggcggcgag cccggtcatc
     4921 actcagcatc tgaacggaaa gaagctccgg ccaggcccgc accgcatcca gggcatc
//
LOCUS       DASREK010000133         5548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1057161, whole genome shotgun sequence.
ACCESSION   DASREK010000133 DASREK010000000
VERSION     DASREK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057161"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFA54_06575"
     CDS             complement(<1..634)
                     /locus_tag="VFA54_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50503.1"
                     /translation="MVSSKPLLRLSPATLLALLGLIFLYSLANFDWFVRNWNVCLLSL
                     ALLLLVYRLFTPAYEFAPALERSLKWPLIFLIFYVAFQLAPLPEPVLKVFSPSRARLL
                     DNLRPLAMGGAFAPLSVAPWATLRELVRMISYVVVFLLVREASWRLPDRPWAPTLPLV
                     IAAGLEAALGLAQYASGDPGSYARGTYVTRDHFSGLLEMCLPFAIAYPVAI"
     gene            883..2103
                     /locus_tag="VFA54_06580"
     CDS             883..2103
                     /locus_tag="VFA54_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50504.1"
                     /translation="MKFLIPVISIVLVGYSLAGIFMEPRFSNDLGLEKNSCRYLLCGD
                     NVLLRRTYETLWNGGPAGPRLAAQGFREALVRDMAFPYRWCDLGQSLADSGQIDQADY
                     CFARAAELGPHVPPILLRVANFYFAVQRPEKALVYASRVLEQGAAYDNLVFGTYSRLG
                     VEFNSILKHGIPEDRRAAQSFLKYTMQSGSPAQAQQAWKWIAGHSFADDPLANQYVEY
                     LVRRKLYAEAAAAWSAYVRQRDGDYPEANFLFNGDFEKPPTGCVFDWQMLGSSGFQAA
                     RDSKTAWSGKWSVRLHFTGKENVTYAGLAQTAHIRPGRYIFEAHAKTSGISTDEGIRF
                     RIVDAEASHHLDVLTEQVLGTSDWREIRKDFTVGPATRLIEIQAVRRPSSKFDSQIAG
                     TAWVDGLKIKAARP"
     gene            complement(2145..2726)
                     /locus_tag="VFA54_06585"
     CDS             complement(2145..2726)
                     /locus_tag="VFA54_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50505.1"
                     /translation="MGRLILALAVCMTSAVAATPAGSITSSGRVELSGISVDARLLPS
                     LPVVLGDEIATSKDPAVLVLLDRSRLILDANSRVKLDAKGGRTVVQVLEGAVNYSLTS
                     SSTTEVVTSGSQATPKSGTRGLLAVGNRTPAPSVLGSGFFKGATPFRTVRTKSCPAPQ
                     PKDDACVAADCGGVCTATCACAACDNNCGLGNN"
     gene            complement(2813..4276)
                     /locus_tag="VFA54_06590"
     CDS             complement(2813..4276)
                     /locus_tag="VFA54_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS50506.1"
                     /translation="MHSTATPAARRFHTLMLVLLSGVLAYAVFRAGGVLRRDQYVFLF
                     VLGLLGSAYSLFAVVNRLPCKVDRALLWPLILLPAYGALQLVPLPVTLLRLVSPARAS
                     LVQALAPVVPGLRFASLSVYPSAGLNRLLVMGGCILIFLLAYQLSVSLGNQRWAAAAP
                     IVIIAAAEAALGVAQFSGSGMDGGTGTYGNRNHFAGLLEMALPLASMYPISIWRRAEG
                     RQPPARAVAAVCAASGAAVLIFLGIICSFSRMGLIACLGSLACLSALAFRQGSRRSRR
                     WLVAAALAAASVAGAASLTPIRFIERFGKISSKPAAEADLRPQLWRDTSRLIAAYWTV
                     GCGLGAYEPAFTKYKVVDPSGAYDYAHNDYLQILAEFGVIGSLLVAAFVYAVVTRSFR
                     NLGPQFSPDPLTAASMAALAAIMIHSLTDFNLYIPANAMVLAWILGISTAPLAASASY
                     SGRASVLPRNWTKDPHAPIHSLRRQMLVCKTAKAQLH"
     gene            complement(4286..>5548)
                     /locus_tag="VFA54_06595"
     CDS             complement(4286..>5548)
                     /locus_tag="VFA54_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZS50507.1"
                     /translation="AMPTATGRQMQEALANCRAAGVPCKTVPGIGELLSGKVLSGQIR
                     NVSITDLLGREPVMLEEGRIRSAIAGRSLLVTGAGGSIGSELCRQAALFEPRALVLFD
                     RAESDLFRIDLELRGKFPGLDVIPQIGDIQDYQRAHEVIRRYAVDSIFHAAAYKHVPM
                     MEAHVLEAVQNNVLGTWNMVQAAHQNNVSDFLMISSDKAVNPTNIMGVTKRVAELIVS
                     SMHGSGTKFVSVRFGNVLGSNGSVVPLFQQQIAAGGPVTVTHPEVRRYFMTVREAVQL
                     VLQASTMGKGSEIFVLDMGEPIRIADLARNMIRLSGRIPDEEIEIRFIGLRPGEKLYE
                     ELITEGEGVQPTYHQKIKIFQGPPVDHTLIEMAVLELGTLVAERDEAGVIALLHKLVP
                     EYQPGKTLKRPPAEQMAAPAASAGRLRA"
BASE COUNT         1222 a         1612 c         1675 g         1039 t
ORIGIN      
        1 ggatggcgac cggataagcg atggcgaacg gcaggcacat ctcgagcaag cctgaaaagt
       61 ggtcccgcgt aacataggtg ccgcgcgcgt agctccctgg gtccccagag gcatactgcg
      121 ccagacccaa ggcggcttct aagccggccg ctatcaccaa ggggagggtg ggcgcccagg
      181 gacggtcggg caggcgccac gatgcttcgc gaaccaggag aaagactacc acgtaactga
      241 tcatccgtac cagctcgcga agcgtggccc acggggcgac gcttagcggc gcgaaagcgc
      301 ctcccatcgc gagcgggcgt aggttgtcga gaaggcgggc gcgggatgga gagaatacct
      361 tcaacaccgg ctccggaagt ggcgctaact ggaaggccac atagaatatc agaaagatca
      421 aaggccattt cagcgaccgc tccagggccg gggcgaattc gtaggcgggt gtgaaaagcc
      481 ggtagacaag cagaagaaga gcgaggctta gaagacaaac attccagttc cggacaaacc
      541 agtcaaaatt ggccagactg taaaggaaga tcagccctag cagcgccaaa agagtagcgg
      601 gggacaaacg caggagcggc ttcgacgata ccataaccac agtttccgcc gaactaaacc
      661 ccggtgcggt gctcttcttc aagggcggcg tccttacggc tttctaacta gcaaaatcag
      721 cccggaatcg taatacagac acatgacggt tgtcaaacgg ttctgcggag ttccattggg
      781 cctttgccgc catgcgggct cgttaaagtt ttttttgagt atctatgtct taatggtata
      841 actagctgat atcatccaga gggcaaagga acgcagcgat ccgtgaagtt tctcattccg
      901 gtcattagca ttgttcttgt cggttactcg ttagcgggaa ttttcatgga gccccgcttc
      961 tccaacgacc tgggtctgga gaaaaattcc tgccgctatt tattgtgcgg cgacaacgtt
     1021 ttgctccgcc ggacttatga gacgctgtgg aatggcggcc ccgcaggccc caggctggcg
     1081 gcgcaaggct ttcgagaggc gcttgtcaga gacatggcgt ttccttaccg ctggtgtgat
     1141 ctgggtcaat ctcttgctga ctcgggccag atcgatcagg cagattattg ctttgcgcgg
     1201 gcggccgagc tcgggccgca cgtgccgccc attctgctga gagtcgctaa cttttatttc
     1261 gcggtccagc ggcctgagaa ggccctggtg tatgcgtcgc gcgtcctaga gcaaggcgca
     1321 gcctacgata acttggtatt tggcacatat tcccgcctcg gagtggaatt taacagcatc
     1381 ctgaagcacg gaattcccga ggaccggcgg gcggcccaat ctttcctcaa atacaccatg
     1441 cagtcgggtt cgccagcgca ggcgcaacaa gcgtggaagt ggatcgccgg ccattcgttt
     1501 gccgacgatc cattggccaa ccaatatgtc gaatatctcg ttaggcgcaa actgtatgcg
     1561 gaagccgcgg ctgcttggtc ggcttacgtt cgacaaaggg atggagacta tcccgaggcc
     1621 aatttcctgt tcaatggaga tttcgaaaaa ccgccaacag ggtgtgtctt tgactggcag
     1681 atgctcggct ccagcggctt tcaggcggct cgagattcta aaacggcctg gtcaggcaag
     1741 tggtcggttc gcctgcattt taccgggaaa gagaatgtta cctatgccgg cctcgcgcag
     1801 actgctcata tccggccggg ccgttacatt tttgaagcgc atgcaaaaac atctggcata
     1861 tcgaccgacg aagggatccg attccgaatt gtagatgccg aagcttcaca ccatttggat
     1921 gttttgacgg agcaggtcct tggtacaagt gactggaggg aaatccgaaa ggattttact
     1981 gttgggccgg cgacgcggct gattgaaata caagctgtcc ggcggccttc ctcgaagttt
     2041 gacagccaga tcgccggtac agcctgggtc gacggcttaa aaataaaagc agcgcggccc
     2101 tgacggacca cgctgcggtc ttccttcttt ttgacccgga agcgctagtt gttgcccaat
     2161 ccgcagttat tgtcgcaagc cgcgcaggcg caagtggcgg tgcaaacgcc gccgcaatcc
     2221 gctgccacgc acgcatcatc cttgggctga ggggccgggc agctcttcgt tctgacagtt
     2281 cgaaagggcg tggcgccctt gaagaaaccg cttcccagaa ccgagggcgc cggcgtccga
     2341 ttgccgaccg ccagcaaacc gcgagtgccg gatttgggcg ttgcctggct gcccgaggtg
     2401 accacttccg tcgtggaact gctggtcagg ctgtagttga ctgcgccctc caaaacctga
     2461 acgaccgtgc gcccgccttt cgcgtctaat ttgacccggg aattcgcatc cagaatcagc
     2521 ctgctccgat caagcaggac gagaaccgcg ggatccttgg atgtcgcaat ctcatcgccc
     2581 aggacaaccg gcagggatgg gagcagcctg gcatcgacgg aaatgccgct caattccaca
     2641 cgaccggaac tggtgatgga accggcggga gtcgctgcga ccgcacttgt catacaaaca
     2701 gcaagcgcca agatcagacg tcccattagc cacctcctat gtatcagaag tggattctat
     2761 tcctatatag cttcgagcgc aagccctcgg agccgggaac tgcaaatatt tctcaatgca
     2821 attgtgcttt tgctgtctta cagacaagca tctgtcgccg caaagagtgg attggtgcat
     2881 ggggatcctt cgtccaattt cttggcaata cgctagctcg tccgctatag gacgcagagg
     2941 ccgcaagcgg cgccgtggag atgccgagga tccaggctaa gaccatggcg ttggcaggaa
     3001 tatagagatt gaagtcggtg aggctgtgga tcataatagc ggccagcgcc gccatagatg
     3061 ccgccgtgag tggatcgggg ctgaactgag gccccaggtt gcggaagctc ctggtgacga
     3121 cggcatacac aaaggcggcg acaagcaagg acccgatcac gccgaactcg gccagtattt
     3181 gcaaataatc gttgtgggcg taatcgtagg ctcctgaagg atccacaact ttgtactttg
     3241 tgaaagcagg ctcataggct cccaacccgc agccgaccgt ccaataagcg gcaatcaagc
     3301 ggctggtgtc gcgccagagc tgcgggcgca aatccgcttc cgctgcgggt tttgaagaga
     3361 tcttgccgaa ccgttcgatg aaccggatcg gcgtaagaga ggcggcgccc gccacggagg
     3421 ctgccgccag agcggctgcc accagccacc gccggctgcg ccgggaaccc tgcctgaaag
     3481 cgagggcgct caaacacgcc agcgacccca ggcacgcgat cagacccatc cgggaaaagg
     3541 agcagatgat gccgagaaat atcaggacgg ccgcaccgga ggcagcacac acggcggcga
     3601 cagcccgcgc gggcggttgc cgcccctccg cacgcctcca gatgctgatc ggatacatag
     3661 aagccaaggg cagggccatt tccagcaatc cagcgaagtg attgcgattt ccataagtgc
     3721 cagttccacc gtccatgccg ctgccggaga actgggcgac gcccagggcc gcctcggcgg
     3781 ccgcgataat gacgatcggc gcggcggccg cccagcgctg gttcccgagg ctcacggaga
     3841 gctgataggc caacaagaaa atcagaatgc agccgcccat taccaaaagc ctgtttaacc
     3901 cggcggaggg atacacactg agagatgcga aacgcaggcc gggaaccacc ggggccagcg
     3961 cctgcaccaa acttgctcgc gccggcgaaa ccagacgtaa cagcgttacg ggcagcggta
     4021 cgagctgcaa ggcgccatac gcgggcagca ggatcaacgg ccagagcagc gcccgatcaa
     4081 ccttgcatgg caggcggttg acaacagcga atagggaata cgccgaccca agcaacccta
     4141 ggacgaacaa gaagacgtat tgatctctcc gcagcacgcc tccggcccgg aacacggcat
     4201 acgccagcac tcccgagagc agcacaagca tcaaggtgtg aaaacgcctg gcggccggcg
     4261 tcgccgtcga atgcatagcc gcggattacg cgcgcagtct gccggcggag gcggcaggcg
     4321 ccgccatctg ctcggccggc ggccgcttga gggtcttgcc gggctggtat tccggcacca
     4381 gcttatgaag caacgcgatc actccggctt catcgcgctc ggcgaccagg gttccgagct
     4441 cgagcacggc catctcgatc aacgtgtggt ccaccggcgg gccttggaat atcttgattt
     4501 tttgatggta cgtgggttgg acgccctctc cttcggtaat cagctcttcg tacagctttt
     4561 cgccgggacg cagtccgatg aaccggattt caatctcttc atcgggaatg cgccccgaga
     4621 ggcgaatcat attccgggcc agatcagcga tgcgaattgg ctcgcccatg tcaagtacaa
     4681 agatctcgga ccctttgccc atcgtcgagg cctgtaacac gagctggaca gcctctcgca
     4741 cggtcatgaa gtagcgccgg acctcggggt gggtgaccgt aaccgggccg ccggcggcta
     4801 tttgttgttg gaacaggggc acgacgctgc cgttgctgcc gagcacgttg ccgaatcgca
     4861 cggagacaaa tttggtgccc gatccgtgca tggacgagac aatcagttcc gccacccttt
     4921 tggtgacgcc catgatgttc gtcgggttta cggccttgtc ggaagagatc atcaggaagt
     4981 cggaaacgtt gttttgatga gcggcctgca ccatgttcca tgtgcccaga acattgttct
     5041 ggacggcttc cagaacgtgc gcttccatca tgggaacgtg tttgtaagcg gcggcatgaa
     5101 aaatcgaatc caccgcataa cggcggatca cctcgtgcgc ccgctgataa tcctggatgt
     5161 cgccgatttg cgggatcaca tcgagcccgg gaaacttgcc gcggagctcc aggtcgattc
     5221 gaaacaaatc gctttccgcc cggtcgaaga gcaccaaggc gcgtggctca aaaagagccg
     5281 cctggcggca gagctccgag ccgatcgaac cgccggcgcc tgtgacgaga agagaacggc
     5341 ccgcaatcgc cgaccgaatg cggccttcct cgagcatcac cggctcgcgg cccagcaagt
     5401 cggtgatcga tacgttgcgg atttgcccgc tgagcacttt gccgctcagc agctcgccga
     5461 tgccaggcac agtcttgcag gggacgccgg ccgcccggca gttggccagc gcttcttgca
     5521 tctggcggcc ggtggcggtg ggcatggc
//
LOCUS       DASREK010000134        28224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1393209, whole genome shotgun sequence.
ACCESSION   DASREK010000134 DASREK010000000
VERSION     DASREK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 28224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28224
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393209"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(914..1825)
                     /locus_tag="VFA54_06600"
     CDS             complement(914..1825)
                     /locus_tag="VFA54_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZS50508.1"
                     /translation="MTRRTFLGASLCGVAGVTGGALAPPGMRAGLAAPAPTPRARIRT
                     GICAYSFRQALQAGTMKYDDLVHLAVETGIDGIDMTVYWFPSIADDFLLPLRRLAYKN
                     GVEIYSLGVRVRLCQPTDELREKELAELRKWVDVAQKMGASHVRVFGGAQPKGASMDQ
                     AISWAAETLKRGAEYSGARGILLGVEDDGGITDHAAETIEIVKRADSPWAGMNLDTGN
                     FKPPKVMEQIDMSIPYAVSSHLKTEITLDDGSKARADWDRILGMYARHGYKGHVGLEY
                     EAAEDPKIAAPRELRRLRDLAQKYSAV"
     gene            complement(1871..2896)
                     /gene="plsX"
                     /locus_tag="VFA54_06605"
     CDS             complement(1871..2896)
                     /gene="plsX"
                     /locus_tag="VFA54_06605"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZS50509.1"
                     /translation="MYTVAVDAMGGDAAPRVEVEGAILAARSYPVKILLVGRQEVLRR
                     ELAEHLADHFPIEIVNATEVITMDDAAAKAVRSKRDSSIRVAARLVRDGRAQGLVSAG
                     NTGAVMATAKIVLGTLPGVHRPALASVFPTLRGTAAVMIDVGANVDSTPQMLAQFAVM
                     GEVYSRVICRLERPRVGLLSIGEEEHKGNELTRQASPLLKALPINFIGNVEGRDLYSG
                     EADVIVCDGFIGNVALKVSEGLVETIKFMLQDSLTATVTRKIGYVLAHAAFDDFKKRV
                     DYSEYGGAPLLGLKGVCIICHGRSNPNAIKNAIRVAMEFSNGRINETIEAALHGRPQE
                     PVRAAGD"
     gene            complement(2911..3093)
                     /gene="rpmF"
                     /locus_tag="VFA54_06610"
     CDS             complement(2911..3093)
                     /gene="rpmF"
                     /locus_tag="VFA54_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZS50510.1"
                     /translation="MPNPKRRHSKARTSKRRAHDALKRAGLAECPNCHEPKLPHRACP
                     HCGQYRGRDIIEVETE"
     gene            complement(3172..3699)
                     /locus_tag="VFA54_06615"
     CDS             complement(3172..3699)
                     /locus_tag="VFA54_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZS50511.1"
                     /translation="MFFDIQTLERHKIPFDEAFPPGSIDFLDESVHQTGEICAEGVAE
                     LIDPFGVREIRVRGSVRGELEVLCARCLESVRLSVSSPLDLFYHPMSEIAANEEVAIS
                     RAETEIGFYEGGGIELADVIREQIAMELPMRCLCREDCQGICPICGTNRNRGACSCRE
                     DISDPRWAALRNWKQ"
     gene            complement(3931..4734)
                     /locus_tag="VFA54_06620"
     CDS             complement(3931..4734)
                     /locus_tag="VFA54_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZS50512.1"
                     /translation="MTKFLTAVAIISLAATATSGCATRRYVRNRTDPISQQVGELDKR
                     TSENARNIGQLDEKTQRDIARVDEEAKTADAHAGDASKRADDALAKGSEAGQKADAAR
                     TVAENSLTRTGELQKYVENLDNYRVSASKTVYFGFDKSVLTDDTKKDLDELAQTLSGA
                     SRYVVEVQGFTDTTGSNEYNYALSEKRANAVVRYLTERHNIPVYRIHTIGLGKDVPAA
                     GTNPREARKMSRRVEVKVYTPQDLPRTAEQSTASQPMTAQSSPAGSPQR"
     gene            5127..8489
                     /locus_tag="VFA54_06625"
     CDS             5127..8489
                     /locus_tag="VFA54_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50513.1"
                     /translation="MDVRVGSQGLLRAAVGMMALCVVSLLLAPVAEAQVLYGTLVGNV
                     TDASGGAVVGAAVKVVNTGTQLVRDTTTNDAGVYTFQNLPAGTYDLTVSASGFTTYTQ
                     LGIILNVNTVQRHDLTLQVGQVAETVKVEASAVTLQTDKADVSAEIPATIIQDLPLPR
                     YRNFQSLINLVPGTTPARFQNANTDSPQRSLATNVNGTSRRDNSTRIDGTLTMQLQLS
                     HAMYIPAAESIEVVNVTTDAADAEQGLSGGSSITMTTKSGTNQFHGSAFGYHMDNAFI
                     AKDYFFKGARTPKNIINDDGATIGGPIKRDKLFFFASYDIIKERTNFNLLYTLPTANQ
                     RAGDFSQYPNTAIFDPGTGDSAGRGRTQFPNNIIPQSRLNPISLKFQSLIPATNQPGI
                     INNFFNSGNDITNRDQVEGKGNYNLSPTHTMFLKYGLFKAAIRGLGGLGEAQGQCLCT
                     GGATGKGAGNTLVQTSTFGQTKTFSPNFIYDMTIGWSREAQTVDQTLRGTNYALDVLG
                     IPGTNGPNINQSGHIRIDLNNQYSGLGNFDNWMPAERHEMVYDMTHNFSLQKGTHSLR
                     WGFEGIHNRLNHFQPGPATQGNAFFTDGMTALNGGPPTNQFNTWAAYLLGTVQQWVKG
                     VQWEEAYAIDWQFGTYFRDRWQVTPKLTLSLGIRYELFPMMHRAGAHHPGIENYDPNT
                     NLVYLGGNGNNPSGLGITTSHKLFTPRVGIAYRLNDKTVIRTGYGINIDPQPFLGWNR
                     AQWPDAITGTFTGPNSFTPFDTWQEGIPPIVGPPAGSPTAVADPKASITFFPKTVVRT
                     YFQSWNFFVERQLPSEIVFALGYVGTESTHVRGSIEENFALPGTGTAGQPFNIKFPGR
                     TASTSFSDGMFSGNYHSLQTSINKRVTNGLTLKGAYTWSHALGMYGTGDTGASIVFMV
                     PELRRRNYGNSLFDVRHNLQLSYIYDLPFGKGKRYLTNGLVSHVAGNWQLNGIFAAYS
                     GRPFNVTSSATACNCPLAVTSGNFTADQINPIVKKLGTPDQWFDKTAFAPVTRRTGTL
                     ADFGNMNLYQLRAPGMVNLDTSVFREFSLTERFKLQFRTEFFNITNTPHFNAPDGNAS
                     ASTFMVINSSFGSRFAQDATNRIIRFALRLHW"
     gene            8681..10771
                     /locus_tag="VFA54_06630"
     CDS             8681..10771
                     /locus_tag="VFA54_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50514.1"
                     /translation="MRNTGIALAAVVLALGPSIRSASNPSHQQFQQAIDLLQKKDNPA
                     AAAVVFENLSHGPDRDLAAQALLYLGECRERAGRQRAIDTYRRILREFADRPEAAAEA
                     RRRIALLESGSSPSAGIVIRKIWALAPDNSGNPSADGRLLTFVDWETGDVAVRDLATG
                     KNRRLTQKGAWRDSPEYAEWPKISADGKKVAYSWATANYGHQLRVVETDGPSGAQPQI
                     VLQNPDLHYIIPWAWSSDDKSVIVLLSRGDDTNQLAWVSVPNGAVRPLKSLEWQSPTS
                     VSLSPDGRFLVYDLPQSNEGPEQDIFIMAADGSREFPLVRHPADDYAPVWTPDGSSVL
                     FASTRTGAVGIWGIPVAEGRPAGPPELLKGGLGQITPLGITRRGALFYAEHRGMDDIF
                     EAEIDPATGKVRGSPVRLVETFIGRNSAPVWSPDGRFLAYFSNRNPAHAYGVGTADIV
                     IRSMEGGAERIFSGASLDLRQAVRWMPDGRSLLVTAKGTDTRARAAFSLVNLATGEFR
                     IVTRSNVTLPFAAVHPEGRSFFVAGGDQNTNLGTITSYDVATGEGREVYRAPAGWVLR
                     NLALSRDGRMLAFSVADPELQRNVIYTMRSTGGAPREVVRLTNSIAREGLAWSVDGRY
                     LFLVETGRAGGAELFRVKAEGGAPETTGISARGLSLISPHPDGAHIAYTVGQYFQIDV
                     WDLENFLPALPAFR"
     gene            10847..11371
                     /locus_tag="VFA54_06635"
     CDS             10847..11371
                     /locus_tag="VFA54_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50515.1"
                     /translation="MSKAEFNKAAATAAPGGLFTPAEQSAIDAGVRQIQMGWIKDPKG
                     LIEWRRALYSLYDLLAEMLLAKGMITEQALEEVIPALVSDILVEKRWRLSITAAGVEN
                     RDLQHPEQFGRFWFHSGYAVTMKRALIDPIRRWKLRIRPRQAVRRSAGQRPVAFRSPA
                     AVRRARKEEKSAAG"
     gene            complement(11368..12525)
                     /locus_tag="VFA54_06640"
     CDS             complement(11368..12525)
                     /locus_tag="VFA54_06640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS50516.1"
                     /translation="MSTAFLTHSGPKQTVIQAASRILVVGGAGYIGSVLVRDLLADGY
                     EVRVLDSLIYGEESLQDLASHPRFALRRGDFRRVEDVVRAVRGVDAVIHLGGIVGDMA
                     CAFDENQTLQTNVAATRLLAEVCRALEAGRFLFASTCSVYGAASSPVDERSALKPLSL
                     YAASKADAERLLLAARSPGFHPSILRLATAFGWSFRPRFDLVVNLLAAKAAVEKQIVI
                     YNREQWRPFIHVADISRAFRAVLAAPVKRVSGEIFNVGSNRMNATLGQLAERIAAVST
                     GLQIDYVDNPDARSYRPSFEKIRQALGFECAMSLESGIREIQSALRCGLITSYEEPRY
                     HNAKQACLRRGELPKEKEYPFTALAFAPNSAWSGREGAAATGQGTGAAPAV"
     gene            complement(12872..14533)
                     /locus_tag="VFA54_06645"
     CDS             complement(12872..14533)
                     /locus_tag="VFA54_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50517.1"
                     /translation="MPYAILRGKMLYRDLWYTYGPLAPYSNALLFYLFGTQLRVLYLW
                     GLTQTLLVALTLFAISRRLLPLGAAFLAAGCFLLQAFQIDLFNFVLPYAYATTLASVF
                     SLLALYFCVRHSLGEPGRNLCGASACAALALLTKQEFGAASYLTVAFLISADAISGRS
                     ARKLASGFLVCLPGLLVNAGAYGWFAWRLSPSFLFLENLNSPFSYHMRAVGAQWIRRY
                     GLRLDLQDIVSSMVTAVLAMAGWFVVAFFFRQLSARRWALPALGASLFTACAALRLFG
                     RQVSGPLYLYGLPLLMFPSGMTWLAVGALFLSLFQWRRNRFDRRRLSVAVVCFYALLI
                     GVRIAFLVLPIYYGVYCSQALFVVFLVVLVRITAGACEDLKTLRSLSVRRCVLAAEAF
                     GLFLTLLPDPGVHPALVRTKHGDIYGTPAESAVYRQLLSFMEQKKEAGQRVVVLPQEI
                     SLYFFTDTLAPTRWYGLQPGVLEPEGREREYVAQLEKEDIDYILLSNRKTGEYGLPDF
                     GVDYCRTVYHWIEQNYEVVGEFGRFVGGTEDDYGVLIYQRRLRPK"
     gene            complement(14890..16272)
                     /locus_tag="VFA54_06650"
     CDS             complement(14890..16272)
                     /locus_tag="VFA54_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50518.1"
                     /translation="MTAGSAAHPGGAVPDPRRRKRLACLLLFSCIFLYNFTRHYLGTS
                     DTVPNMYLPVSIILHGDFYLDRFPTLPRINQRPLPYFLQFRNGRVISSYPVLPAIMAV
                     PVYLGPVLWLKAEGISYGSPIFSEMCIFSAKLAASLIAACSAVFVFLTLAAYASQSKA
                     FALAHLYAFGTSAFAIASQALWMHGPAMLFLSIAAYLWLCRPKRLIAAGAMLSLAVAA
                     RPVMMVAAGIFTMYVAMRRPRELPRFLAVPAVVAALLLAFNLHFYNSLSGGYREVNAD
                     MLQHQRLPSVWTSAIPTGLAGLLISPSRGLFIFSPFLLAGFWGGIRVLRDSRWREWQP
                     LALSVFGQVLLFSCYSSWWGGMCYGPRLLSEACPYLVLLIAPVIPEIDAKRLRRVTFQ
                     VLAAASIFVQTAGAMLWTGNWYADPTDVDIDHARLWDWKDNEITRTLRNYRWSPESPH
                     KPPSTESLPR"
     gene            complement(16269..17402)
                     /locus_tag="VFA54_06655"
     CDS             complement(16269..17402)
                     /locus_tag="VFA54_06655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HZS50519.1"
                     /translation="MKQSSLTPAARRGQRVLEFTAATLIAAIWIGFVFWQSAVRKVAA
                     DFAPFYCAGKILRAEGPHALYDWAVIREQQMRLFPEERRTSPFIRPPFYALVLSPFSL
                     LSPGRALLVWLSLNILALVVALFLSARAMNMEFSALSWHTAIFFPALYAVFNRQDVPL
                     VLLLAAAAYLLLARDRPFSAGLALSLCEIKFNLLLLVPLCFLIRKNWRALAGFAVGSS
                     VLLLISTFLVGPAGMIEYARMLIQRTPPQMEFPERTANVASLVANTWVQVPLILSVAA
                     AVITAARRLPQLEAVSAAWLGSLLIVPHVFISDYLLALPALLLLARLNPACLFSSLVV
                     LFPVTPIFMVRGDRFLLLTPLMVLVCLAGSAFLPAKSWKTAVQ"
     gene            complement(17668..18603)
                     /locus_tag="VFA54_06660"
     CDS             complement(17668..18603)
                     /locus_tag="VFA54_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZS50520.1"
                     /translation="MSLLILGLVWFVLVAGVISLVGYRYWVKPAAYMDRVGAQTFDHL
                     ADYIEDGPDELGVTKLVQSLGEAVPVSPSTVRRLRRRLITAGYRSESAVAVFSGLRVL
                     CAAGLALLSAIGLAITHLLSGQMILALSLLAGLLGYALPNEILLMLIQRRRRILRHSL
                     PDALDLLVVCVESGLGLDQAILDVSKELSITHPQLSDEFSLVTLELKAGKRRSEALRN
                     LADRTGEDEFKKLVSILIQTDRFGTSIAQALRSHAEFMRVKRRQIAEEKANKVGVKLV
                     FPIFFFILPSMFLVTVGPALLELIFEFFPWMKTVQ"
     gene            complement(18606..19577)
                     /locus_tag="VFA54_06665"
     CDS             complement(18606..19577)
                     /locus_tag="VFA54_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZS50521.1"
                     /translation="MLPTIILVTAFLMIFAVSAIAASAAWNVVEKRRQKALDSRLQRT
                     IEQDEEADPDLLKKDTTSILPFYGQLLEKFAFTRTLQQYLAQANLKWSVGLLTAMMLV
                     TAFLALNILIRLSWFPGYLIPPAVLLAGMIPYGYVMRKRSKRLAKFEEQFPEALDFLS
                     RAMRAGHAFSISLELLSEEAMDPVSTEFRRTFDEHNLGLPLENALQNLAVRVPLLDVR
                     FFVSAVMLQARTGGNLSEILLKLAQVIRERFKLRGQVRALSAHGRLTGKVLTVLPVVV
                     VLLMMVVNPEYMVALVKFPIGKHLIGAAIIMQGIAFLFIRKIVNIEV"
     gene            complement(19608..20954)
                     /locus_tag="VFA54_06670"
     CDS             complement(19608..20954)
                     /locus_tag="VFA54_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZS50522.1"
                     /translation="MATSTFHSPSGTSWEDRLLLRSNPSAEHLERKFALHRKLLDKIN
                     LEKLSSINGQRMRNEVRAAVTGLVEEEKTPLSLVEKEQLIEEVLDEVFGLGPLEPLLQ
                     DPTISDILVTTPRLVHIERAGKLYLTPVQFKDDAHLLRIIEKIVSRVGRRIDESSPMV
                     DARLPDGSRVNAVIPPLAVDGPLLSIRRFGRDPLTHEDLVKNLTLTKDMLQLLKGCIQ
                     ARLNIVISGGTGAGKTTLLNALSAFIGVDERIVTIEDAAELQLKQIHVARLETRPPNI
                     EGQGAVRQRQLVINALRMRPDRIIVGEVRGEEALDMLQAMNTGHDGSLTTIHANSCRD
                     AISRLETMVGMANSNISPKSVRQQISSAVDLFVQIARLSDGTRRVTHITECVGMEEDT
                     ITLQDVFVFDRLGLTNERRVIGQFRATGIRPKFAQKLKASGIQLPPAMFETITHIQ"
     gene            complement(21000..22160)
                     /locus_tag="VFA54_06675"
     CDS             complement(21000..22160)
                     /locus_tag="VFA54_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS50523.1"
                     /translation="MSLALILERPSFREQVLQELKEIPVRVVLDQGGFGAWHLVHERI
                     ERLQPDVILADLGEQPDERFSYIQLMRTMSHPPAVVVVHDSSDAGVILKAMRAGAAEF
                     LTMPLEPGALVNALERISAIAPGASGSKAAGGRLLAFVSVKGGAGATTLACNVAAALG
                     RSTKQEILLADLDLETGNVAFAMKAGSQYSILDACRNISRLDVHYWKGLISNGQPNLN
                     ILTAPSDLRGFDNPQGVEVRQVLGFARTIYAYSVLDMPSSLSRLTLAALEDADKIFLI
                     TTTDLPCLHLAKRALQKLNAAGHPLDRVALVVNRSSRHDEVLPEDIERNLGVPVFWRF
                     PNDAKSVTEFYVKGGVISPNSDLGKSITKFVAKISGPPSLPEAGKKWSLLGL"
     gene            complement(22166..23554)
                     /locus_tag="VFA54_06680"
     CDS             complement(22166..23554)
                     /locus_tag="VFA54_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HZS50524.1"
                     /translation="MTLKAKRLGSWKFWWKAALVWTACAALCFGQAAPAPQATRELSV
                     LVGKSVMIDSTPIIQRVSVATPEVADAIIVDPHEVLINGRAAGATSLILWQEGGGRIF
                     FNLTVRPDLQPLAQQIQESFPDGNVHLSYENNTWFLRGTVRDTQAAERAQAIAATAGK
                     VVNLLQVEAPPAEPQIMLKVRFADVDRAAVQELGANIISTGALNTIGSTTTGQFQGGL
                     QRNQIGGANAAQVPFTVTDLMNIFAFRPDLNIAATIHALEQKRLLQILAEPNLMTVNN
                     REATFLAGGEFPFPVVQGGAVAGAVTIQFREFGVRIGFLPVITPRNTIRLRVRPEVSS
                     LDFTNALTFQGFTIPALNTRKLDSEVELADGQSFIIGGLLDNRVIESFSKIPGLGDIP
                     IIKNLFRSKSLTRSNTELMVLVTPEFVRPFNEGEAPPPPKMDRPNLEIPGQHPAENPR
                     VKGGAGAQGANR"
     gene            complement(23591..24487)
                     /gene="cpaB"
                     /locus_tag="VFA54_06685"
     CDS             complement(23591..24487)
                     /gene="cpaB"
                     /locus_tag="VFA54_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZS50525.1"
                     /translation="MNKRLLTVIGFSLCVAAAATLLLYSLISSRLHSGAPAAPAQQIV
                     VASRDLHRGELLKDSDIGVVRWGTSILPKGSFTDKSNVVGRGVLAEVATGEPVLESHL
                     APREAGAGLAPAIPIGKRAVSVRVNEVVGVAGYVLPGSRVDVLVTGNAPGQNQQGPEI
                     RTVLQNVEVLSAGTNIQHDAEGKPVQVPVVTLLVTPEEAEVLSLASNETRIQLVLRNP
                     LDSNKAETKGTAVARLFRTGPAPSDTPAPPKPRKVVTKMVAPATPPPPPPPPPIVVEL
                     LKGPSRTSEKFKTEDTPGEKLQ"
     gene            complement(24513..24698)
                     /locus_tag="VFA54_06690"
     CDS             complement(24513..24698)
                     /locus_tag="VFA54_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50526.1"
                     /translation="MRELLGRFTQEQDGQDTMEYTLLLGFVALAAAAIFPVFANNLKT
                     IWQIASNHLSNTASAAK"
     gene            complement(24805..25038)
                     /locus_tag="VFA54_06695"
     CDS             complement(24805..25038)
                     /locus_tag="VFA54_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50527.1"
                     /translation="MSEAITNPKEIESMRKTKNILCSFWKHQDGQDTMEYTLLLGFVA
                     LAAAAIFPFVTSNIKTIWQIASNHLSNAASAAK"
     gene            complement(25151..25345)
                     /locus_tag="VFA54_06700"
     CDS             complement(25151..25345)
                     /locus_tag="VFA54_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50528.1"
                     /translation="MTYLKFLARNFWRGEDGQDTMEYTLLLGFVALAAAAIFPFVTSN
                     IKTIWQIASNHLSNAASAAQ"
     gene            complement(25422..27038)
                     /locus_tag="VFA54_06705"
     CDS             complement(25422..27038)
                     /locus_tag="VFA54_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50529.1"
                     /translation="MPQNATARRSLLPSLSDMIFSTTLAFLFLFGKGATTLLGDSDTG
                     WHIRTGEWILQNHAVPRHDLFSFSKPGGEWFAWEWLSDVLFALVHRYAGLAGLVLLAG
                     LVIALVFTFLYRFMVRRGANVLLATALAVTASYAASIHWLARPHIFSWAFALIFYWVL
                     EEAPVERNAIFWLAPLTILWTNLHGSFVVGLFLIALYGAGELVQAALAAGEDTAAIRR
                     GALRRAGRYALLLAVSGAASLVNPYFYKVHVHIFGYLKNAYILDHITEFFSPNFHAAP
                     ERFFEILLLGGIASALWAWRQGRYTEALLVVSFAHFSLQSARHVPLYAILAAPLIAAA
                     LTDSLAPHHTASWAAWARGLAGAFGRFGEDIARMDRAPRVYAASAMVTLLVVAMLWSP
                     ASSAGLQDFHPRFNPKLFPVAAADFLERSSFSGNVFSTDQWSSYLIYREFPRVRVFMD
                     GRSDYYGPKLGELYLDLINVHHAWERTFQQYGVETVVLPVDSGLVGALKESPHWKVAY
                     DDGIAIVFERAASAPARLVASVRGAGRREQ"
     gene            complement(27040..>28224)
                     /locus_tag="VFA54_06710"
     CDS             complement(27040..>28224)
                     /locus_tag="VFA54_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50530.1"
                     /translation="LLEIAQDGSVQGKVAPSSSGVKFINVPQVPDPTLSMNRGNQPTV
                     PTLQQVVNNTPGQVVYGVPGGFSNTTLTPGAYIGGTYNPSSKLFDISGAPITLTNVTF
                     NSGGKPGTYIFFGGLNLAGNVKLDQGTYVIAGVSNASNPILSLQGSSQLNLTDGVSGP
                     GPGSYRGEMLLLTRPDYTPDGSGSNAVKLLPPAYSPPIQAMTSLFTVQAGVPAVQPSP
                     GTPWAAVANWGFGGISESNSGKGNLSVNITGLENYPACDPGVCESPATNPLNNSNYLY
                     QPAIVWVDRRNTPLANSAINSAGLPWPTPGGSVCPTATFLAPCPTQNTATWDAQGSPP
                     AGFNGMIYMPQGSLMNMQGGGATSQPTLIITGAMSLNGNATIALVSPKTPALQTLVSL
                     IR"
BASE COUNT         5919 a         8590 c         8319 g         5396 t
ORIGIN      
        1 agtccaggac gcggctggat tcgcttacgt tggccgcaag gaagtcggcg ctttcctcag
       61 ggaaaacgtg ttcgctccgg gcgcccgcta ctcctggaat gagctgacga agctggctac
      121 cggcgcgccg cttcaggcca acgactacgc tcaggatttt gtgagcgagt aaacgccgca
      181 ggccgcggac gaaccgactt tctcgccccg cccgcagcgg aagccgcggt ccatcagccc
      241 gagcctgggc cagccaggga tcggctgatc gccccgcctc gcagctatcc actccccccg
      301 cggtcacccg ctttcctgtt ttttcttccc cctgcctgca atcacttgcg ggcgctggcc
      361 gccttcggcc atcccacccg cccttatcct gaagctgatc gcgagcgtgg gcccacccgc
      421 tccgtagttt ggtgttttgc gcctctggcg gcggtaagac cccaaggtag gggcagccgg
      481 cagcgtaagt ccccgaaact agggcgcccg cacagaagct ggccgctctt cggacgcccg
      541 gggttgcccg ggtggcaaga gaggggcggg acaagcagtc catcgatgga ccaagctgga
      601 tctgcaaggc agcctagccg gtgaccggcc cgacgctgaa tcgggcgccg attcacctct
      661 ctcgctagcc ggccgtgaaa ggctcgccgg gaccgatcct cgcaccccgg ctattggaga
      721 gacgtcctct cctttagccg gctcggagca ctctttatcg cccttccccc accctttaca
      781 cccgtagtgt ctctgctaca cccgcagcgt ccctcgccct gccccggccc tgaacggtga
      841 tcctgagcac agcgacgttg ttcctaagcg atgctgttcg taagaaaact tgacgatctg
      901 cggcggcagg agttcacacg gcggagtact tctgcgcgag atcccgcagg cgccgcagct
      961 cgcgtggcgc cgcaatcttc ggatcttcgg ctgcctcata ctcgaggccg acgtggcctt
     1021 tgtatccatg gcgggcgtac atgccgagaa tccggtccca gtcggcgcgc gctttcgagc
     1081 cgtcatcgag cgtgatttcg gttttcaagt ggctgctgac ggcatacgga atcgacatgt
     1141 caatttgctc catcactttc ggcggcttga aattccccgt atccaggttc atgcccgccc
     1201 agggcgaatc ggcgcgcttg acgatctcaa tcgtttcggc ggcgtggtcg gtgatgccgc
     1261 cgtcatcttc caccccgaga aggatgccgc gcgcgccgga gtattccgcg ccgcgcttca
     1321 gcgtttcggc ggcccagctg atcgcctggt ccatcgaggc gcccttgggc tgcgcgcccc
     1381 cgaaaacacg cacatgactc gcgcccattt tttgcgccac atccacccat ttgcgcagtt
     1441 ccgcgagttc cttttcccgc agctcgtcgg tcggctggca cagccgtacg cgaaccccga
     1501 ggctgtaaat ttcgactccg tttttgtacg ccaggcggcg cagcggcagc agaaagtcgt
     1561 ccgcgatgct gggaaaccag tacaccgtca tgtcgatgcc atcaatgccc gtttccacag
     1621 ccaggtgaac caggtcgtcg tacttcatag tccccgcttg cagcgcctgg cggaacgaat
     1681 aggcgcagat tccggtgcgg attcgcgccc gcggcgtagg agcaggggct gccaagcctg
     1741 ctcgcatgcc gggcggcgcc agagcgcctc cggtgactcc ggccacgccg caaagcgagg
     1801 ctccgaggaa cgtgcgacga gtcataacca cctccggacg atcttggagc ggctccccgc
     1861 ccggacagaa tcaatccccg gcggcccgga ccggctcctg gggccggcca tggagagctg
     1921 cttcgatggt ttcgttgatg cggccgttag agaattccat agcgactcga atggcgttct
     1981 taatcgcatt cggattcgag cggccatggc aaatgataca cacgcctttc aagccgagca
     2041 acggggcgcc gccgtattcc gagtagtcca cccgcttttt gaaatcatca aacgccgcgt
     2101 gagcgagcac atatccgatt ttgcgggtta ctgttgccgt tagtgaatcc tggagcatga
     2161 atttgatcgt ctccaccagg ccctcgctca ctttgagcgc tacgttgccg atgaagccgt
     2221 cgcagacgat aacgtcggct tcgccgctgt aaagatcccg cccttcgacg ttgccgatga
     2281 aattgatggg aagcgctttg agcaggggcg aggcctggcg ggtcagctcg tttcctttgt
     2341 gctcttcttc gccgatcgag agcagcccca cgcgcgggcg ctcgagccgg caaatcaccc
     2401 gcgagtaaac ctcgcccatc accgcgaact gggcgagcat ttgcggcgtg gaatccacgt
     2461 tggcgccgac atcgatcatg accgcggccg tgccccgcag agtgggaaac accgaagcca
     2521 gcgcggggcg gtgcacgccc ggcagcgtcc ccagcacgat cttcgccgtg gccatcaccg
     2581 cgcctgtgtt ccctgccgaa accagcccct gcgcgcggcc gtcccgcacc agtctcgcgg
     2641 ctacgcggat cgaactgtcc cgcttcgacc tcaccgcctt cgctgccgcg tcgtccatgg
     2701 tgatgacttc cgtggcgttg acgatctcaa tcgggaaatg atccgccagg tgctcggcga
     2761 gctctcgccg caagacctcc tggcgtccca ccagcaggat cttcaccgga taagaccggg
     2821 cggctagaat ggcgccttcc acttccaccc gtggggcggc gtcgcccccc atcgcatcga
     2881 cggcaacggt gtacatggtg tcggggaatg ctattctgtt tcgacttcga tgatgtccct
     2941 tcccctgtat tggccgcagt gcgggcaagc gcggtggggc agcttcggct cgtggcaatt
     3001 cgggcattcg gccaggcccg cccgcttcag cgcatcgtga gcgcgccgtt tggatgtcct
     3061 ggccttggag tgtcttcgtt tcggattcgg catttcacaa ccctttcttg cgcgaaacaa
     3121 cacgggaaaa gcggaaaatc gaatccccgc tcgtgttgcc ttcctagaat tctactgctt
     3181 ccagtttctt aatgctgccc agcgggggtc cgaaatatct tcgcggcacg agcaggcgcc
     3241 acggttgcgg ttggttccgc aaatcgggca gatgccttgg caatcctcgc ggcagaggca
     3301 ccgcattggc aattccattg cgatctgctc ccgaatgacg tccgccagct cgattccccc
     3361 tccctcgtaa aagccaatct cggtttcagc gcggctgatc gccacttctt cgttggcggc
     3421 gatctccgac atggggtggt agaacaggtc cagaggagaa gataccgaaa gccggaccga
     3481 ttcgagacag cgggcgcaga gcacctccaa ctcgcctcgt acgctgccgc gcacccgaat
     3541 ttcgcgaacg ccaaacgggt cgatcagttc ggcaacaccc tccgcgcaga tttcgccggt
     3601 ctgatgaacg ctttcgtcga gaaaatcaat cgagccggga ggaaaggctt catcaaacgg
     3661 aattttgtgt ctttcgaggg tctggatatc aaagaacata cagccagctc cgcccgaaag
     3721 ggcgttacaa gccttttatt ataccggaac gctgcgctga gggcaggcct catcgacgca
     3781 ccgcagttga ctttggcgcg caagcgggtt tgtggccgcc ggcggggttc ggttccttct
     3841 tccacttgga tgagcggggg ctgcggagca ggcaaggagg cgcgagcagg ggcgcgtccg
     3901 ccgcgcgccc ctgttgcaca ccgccggcgt ttagcgttgt ggcgatccag ccggcgagga
     3961 ctgcgccgtc atcggctgag aggctgtgct ttgttccgcc gtgcgcggca aatcctgggg
     4021 cgtgtacacc tttacttcca cgcgccggct cattttacga gcctcacgcg ggttcgtgcc
     4081 ggcagccggc acgtcttttc ccaggccgat cgtgtgaatg cggtacaccg ggatgttgtg
     4141 gcgctctgtc aggtaccgga cgactgcatt cgcgcgcttc tcactcagcg cgtaattgta
     4201 ttcgttcgag ccggtcgtat cggtgaagcc ctgcacctcg acaacataac gcgaagcgcc
     4261 ggagagcgtc tgcgccagct catccagatc cttcttcgtg tcatcggtca aaaccgactt
     4321 gtcgaagcca aaatagacgg tcttcgatgc gctcacccgg taattatcga gattttccac
     4381 gtatttttgc agctcccccg tgcgggtcaa gctgttttca gccacggtgc gggccgcatc
     4441 cgccttctga ccggcctcgg accccttggc caaagcgtcg tccgcccgct tgctggcgtc
     4501 gccggcgtga gcgtcggccg tctttgcctc ttcgtccaca cgagcgatgt ctcgctgggt
     4561 tttctcgtca agctgcccga tgtttcgagc attctcggaa gtccgcttgt cgagctcgcc
     4621 cacttgctgg ctgattgggt ccgtccgatt ccttacgtat cgccgggtgg cgcatcccga
     4681 ggtagcagtg gccgccaggc tgatgatggc caccgctgtt aagaacttag tcatacaccc
     4741 tcctccgttc ggaatccaac ggtttgcacg cctccagcca atcagcaatc cacacattct
     4801 acggagtttg ccttactcaa gacacccccg gcccgtgact cctttacatg cggccggtaa
     4861 attttgcaaa aacgcggcgg ccgccgcgct gtgctgaaaa tcggcaaaat gccccgcgcg
     4921 gaatcttatc tcaagccggc cttcgcccca gttggttgcc ccgaagtttt acacggccaa
     4981 ctaggaaaga ctctattaca agaaaagttg gcgtttttcc tctagaacct agacaagaga
     5041 tagggttagt ctgctgcacg accaacaggg gatcttaacg ccctgcgggg gtgaggagac
     5101 gggttttcaa aactcaggga ggccatatgg atgtgcgtgt aggttctcag ggtttgcttc
     5161 gcgcagccgt tgggatgatg gcgctgtgcg tggtttcatt gcttctggcg ccggttgccg
     5221 aggcgcaggt tctctacgga actctggtgg ggaacgtgac tgacgcgtcg gggggcgcgg
     5281 tcgtcggcgc cgctgtgaaa gtcgtcaaca ctggtacgca gctggttcgt gatacgacca
     5341 cgaacgacgc cggagtctac acgttccaga atctgccggc cggcacatat gacctgaccg
     5401 tctccgcttc gggtttcact acgtacacgc agttgggcat tatcctgaac gtcaacaccg
     5461 ttcagcggca cgacctgacc ttgcaggtag gacaggtagc cgaaacagtg aaagttgagg
     5521 cctctgcggt aacgctgcaa acggacaagg ctgacgtgag cgccgagatc ccggcgacga
     5581 ttattcaaga tctgccgctg ccccgctacc gcaactttca gagcctgatc aacctggtgc
     5641 ccggaacgac gcctgcccgg ttccagaacg cgaatacgga ttccccgcag cgctcgctcg
     5701 cgaccaacgt caacggcacc agccgccggg acaacagcac gcgcatcgac ggcacgctga
     5761 ctatgcagct ccagctttcg cacgcgatgt atatcccggc ggccgaaagc atcgaggtgg
     5821 tgaacgtgac gacggacgcc gccgacgcgg agcagggcct ctcgggcggc tcctcgatca
     5881 ctatgaccac gaaatccggc accaaccagt ttcacgggtc ggctttcggg tatcacatgg
     5941 acaatgcctt catagcgaag gattatttct tcaaaggcgc gcgcactccc aagaacatta
     6001 tcaacgatga cggcgccaca atcggcggcc cgatcaagag agataagctc tttttcttcg
     6061 ccagctacga catcataaaa gaacgcacga atttcaatct cttgtacacg ctgcccacag
     6121 ccaatcagcg ggccggggat ttcagccagt atccgaatac cgcgatcttc gatcccggca
     6181 cgggagactc ggcgggccgc ggaagaacgc agttccccaa caacatcatt cctcagagcc
     6241 ggctgaatcc gatttcgctg aagtttcagt ccctgattcc ggcgaccaac cagccgggga
     6301 tcatcaataa cttcttcaac tcaggcaacg acattacgaa ccgcgaccag gtggaaggca
     6361 aaggcaacta caacctgagc ccgactcaca ccatgttctt gaaatatggt cttttcaaag
     6421 cggccattcg aggtctcggc ggactaggcg aagcccaggg ccagtgcctt tgcaccggcg
     6481 gagctaccgg caagggcgcc ggcaacacgc tggtgcagac ctcaacgttt ggccagacga
     6541 agaccttcag cccgaacttc atctacgata tgaccatcgg ctggagccgg gaagcgcaga
     6601 ccgtggacca gactcttcgg ggaactaatt atgcccttga tgttctcggc atccccggga
     6661 ccaatggacc gaatatcaac cagagcgggc acatccgaat cgacctgaat aatcaatact
     6721 caggcctggg caacttcgac aactggatgc cggctgagcg ccatgaaatg gtttacgaca
     6781 tgacgcacaa tttcagcctg caaaagggca cccacagcct gcgctggggt tttgagggca
     6841 ttcataaccg actgaaccac ttccagcccg gcccggcgac tcaagggaat gcgtttttca
     6901 ccgatggcat gacagcgctc aacggcggcc cgcccaccaa ccagttcaac acctgggctg
     6961 cttacttgct tggcactgtg cagcagtggg tgaagggcgt gcagtgggag gaggcctacg
     7021 cgatcgactg gcagtttggg acctatttcc gcgaccgctg gcaggtgact ccgaagctga
     7081 cgttgtcgct cggaatccgc tatgagcttt tccccatgat gcaccgcgcc ggcgcgcatc
     7141 acccgggcat cgagaactac gatcccaata cgaatcttgt gtatctggga ggcaacggaa
     7201 ataaccccag cggtctggga atcaccacga gccacaagtt gttcacgccg cgcgtgggaa
     7261 tcgcgtaccg tctcaacgat aaaaccgtca tccgtaccgg ctatggcatc aacatcgatc
     7321 cacaaccgtt ccttggctgg aaccgggcgc aatggccgga tgcgatcacc ggaaccttta
     7381 ccggtccgaa cagcttcacc ccctttgaca cctggcagga gggcatcccg cccatcgtgg
     7441 gcccaccagc cggctctccg accgccgtgg cggatccgaa ggccagcatt accttcttcc
     7501 ccaagactgt agtccggacg tatttccagt cgtggaactt cttcgtcgaa cgccagttgc
     7561 cgagcgaaat tgtttttgcc ttgggttatg taggtactga atcgacccat gtccgcggaa
     7621 gcattgagga aaactttgcg cttccgggaa ccggtactgc cggccagccc ttcaatatca
     7681 aattcccggg acggacggcc agcacttcct tctcagatgg catgttcagc ggcaactacc
     7741 actccttgca gaccagcatc aataagcgcg tgaccaacgg cctgacgctc aagggcgctt
     7801 acacttggtc ccacgctctc gggatgtatg gcaccggaga tacgggagcc tctatcgtct
     7861 tcatggtgcc cgagctccga cggcggaact acggcaattc gctttttgat gtgcgccaca
     7921 atctccagtt gtcttatatt tacgatctgc ctttcgggaa aggcaagcgg tacctcacga
     7981 atggattagt gagccacgtg gcaggcaatt ggcagcttaa cgggatcttc gccgcgtact
     8041 cgggccgccc gttcaatgtg acctcctcgg ctaccgcctg caactgcccg ttggccgtga
     8101 catcggggaa cttcaccgcc gatcagatca atcccattgt gaagaagctg ggcactccag
     8161 accagtggtt tgacaaaaca gcttttgccc ccgtaacgcg ccgcaccggc acgcttgccg
     8221 acttcggcaa tatgaacctc taccagctcc gtgctcccgg catggtaaac ctcgacacga
     8281 gcgtgttccg ggaattctcg ctgaccgagc ggttcaaatt gcagttccgc accgagttct
     8341 tcaatatcac caacacgccg cacttcaatg cgcccgacgg caacgcgagc gcatcgacgt
     8401 tcatggtgat caacagttct ttcggcagcc ggtttgccca ggatgcgacc aaccgcatta
     8461 tccggtttgc cttgcgattg cattggtaaa cggcaggatg cttctcaggc cgggcgcctg
     8521 cgcggcgccc ggctttttct tctccgggca gacgccccag ggaaccgtgc cggcaggctc
     8581 ttcgactccc cggaccggcc gcagctattc tctggagagt ctcccgcgtt ctctaggcgg
     8641 cttgttctgt tcgtagtaaa atcaggctac gaggacgggg atgcgaaata ccgggatcgc
     8701 gctggcagcg gtcgttttgg cgcttggtcc atcgattcgt tccgcaagca acccgtctca
     8761 tcagcaattc cagcaggcta tcgatttact ccagaaaaaa gataatcctg cggccgccgc
     8821 agtcgtgttc gagaatctga gccatggccc agaccgggat ttggcggctc aggcgctgct
     8881 ttatttgggc gaatgccggg aacgagcggg caggcagagg gcgatagaca cgtatcgcag
     8941 aatcctgcgg gagtttgcgg accgtccgga ggcggcggca gaagcccggc ggcgcatcgc
     9001 gttgctggag tccggttctt ctccgagcgc cggaatcgtg atccgaaaaa tctgggcgct
     9061 ggcgcccgac aactccggca atccgtcagc ggacggccgg ctgctgactt ttgtggactg
     9121 ggaaacgggc gatgtcgccg ttcgcgatct ggcgaccggt aaaaaccggc gtttgaccca
     9181 aaagggcgcg tggagggact ccccggagta cgccgaatgg ccgaaaattt ccgccgacgg
     9241 aaagaaggtg gcgtattcct gggctactgc caactatggg caccagctac gggttgtcga
     9301 aacagacggc ccttccgggg cgcagccgca aatcgtcttg caaaatccgg atctgcatta
     9361 catcatcccc tgggcctggt cttccgatga caaatcggtg atcgtattgc tatcgcgcgg
     9421 cgatgacacc aaccaactgg catgggtttc cgttcccaac ggagccgtcc ggccgttgaa
     9481 atctctcgag tggcaatccc cgaccagtgt gagcctgtca ccggacgggc gctttctagt
     9541 ctacgatctt ccccaatcca acgaagggcc ggagcaagat attttcatca tggcagcgga
     9601 tggcagccgg gaatttcctc tggtgcggca tccggctgac gattacgcgc ccgtatggac
     9661 tcctgacgga agcagcgtgc tgtttgccag cacgcgtaca ggagccgtcg ggatctgggg
     9721 gattcctgtg gccgaaggac gcccagccgg cccgccggag cttctcaaag gcggcctcgg
     9781 acagatcacg ccgctcggca tcactcgccg gggagcgctg ttctatgcgg agcaccgggg
     9841 aatggacgat attttcgaag ccgagatcga tccggccacg ggaaaggtgc ggggatcccc
     9901 agtgcggctg gtggaaactt tcatcgggcg aaacagcgcg cctgtgtggt cgcctgacgg
     9961 ccgcttcctg gcctacttct cgaatcgcaa tccggctcat gcgtacggcg tgggcacggc
    10021 cgatattgtg atccggtcca tggaaggggg cgcagagcgc attttctccg gagccagcct
    10081 ggatttgaga caggccgtgc gctggatgcc cgacggcagg tcgttgctgg tgacggctaa
    10141 aggcacagat acccgcgcca gggccgcctt ttctctggta aatctggcaa ccggggagtt
    10201 ccggatcgtt acgcgttcca acgtaaccct gcccttcgcc gccgtgcatc cggagggccg
    10261 ctcgtttttc gttgcgggag gggatcaaaa caccaacctc ggcacaatta cgtcgtacga
    10321 cgtcgcgaca ggagagggcc gcgaagtcta tcgcgcgccg gccggctggg ttctgcgcaa
    10381 cctggctctt tcccgcgacg gccgcatgct tgctttcagc gtagcggatc ctgagttgca
    10441 acggaacgtg atttatacca tgcgctcaac ggggggagcg cctagggaag tcgtgcgcct
    10501 cacaaacagc attgccagag agggtctcgc atggagtgtg gacggccgtt acttatttct
    10561 tgtggagacg ggccgcgctg gtggggctga gttgtttcgc gtgaaagcag aaggaggagc
    10621 gcccgaaaca acgggcattt cggcaagagg gttgtcgctg atcagcccgc acccggacgg
    10681 ggcgcatatc gcttatacag tcggccagta tttccagatt gacgtgtggg atctggagaa
    10741 ttttcttcct gccctgcctg catttcgcta aatgcgggat tcgggcggag gactacggcc
    10801 agcgggccgc ggggggcctt ttggccgaag cggattgggg tgagaaatgt caaaagcgga
    10861 gttcaacaaa gcggccgcaa cggccgcgcc ggggggtctg ttcacgccgg cggagcaaag
    10921 cgctattgac gccggagtcc gccagattca gatgggctgg atcaaagatc ccaagggtct
    10981 catcgagtgg cggcgtgcgc tctatagcct gtatgacctg ctggcggaaa tgcttttagc
    11041 caaggggatg ataaccgagc aggcgctcga agaggtgata cccgctctgg tttccgacat
    11101 cctggtggaa aagcggtgga ggctgagcat taccgcagcc ggcgtagaaa accgggacct
    11161 ccagcatccc gagcagtttg ggcgcttctg gttccacagc ggctacgcgg ttacgatgaa
    11221 gcgggctttg atcgatccta tacgccggtg gaagttgaga atcaggccgc ggcaagcagt
    11281 tcgccgcagc gcagggcaac gcccggttgc tttccgttcg cccgcggcag tacgccgggc
    11341 gcggaaagaa gagaagagcg cagccggcta gacggccggc gccgctccgg taccctggcc
    11401 tgttgcggct gccccttccc ggccgctcca ggcgctgttg ggcgcaaacg caagggcggt
    11461 gaatgggtat tccttctctt tgggcagctc tccccggcgc aggcaggctt gcttcgcatt
    11521 gtgatagcgg ggctcctcgt agctggtgat cagcccgcag cggagcgcgc tctggatttc
    11581 ccgaattccg ctttccaggg acatggcgca ctcaaatccc agcgcctgcc gtattttttc
    11641 aaagcttggc cgatagcttc gagcgtctgg gttgtcgaca taatcgattt gcaggccggt
    11701 cgagactgcg gcgattcgct ccgcaagctg tccgagggtg gcgttcattc gatttgaccc
    11761 gacgttgaaa atttccccgc tcacccgttt gacgggtgcg gcgagcacgg cccgaaaggc
    11821 gcgggagata tcggcgacgt gtataaaggg tctccactgc tcgcggttat aaattacgat
    11881 ttgcttctct accgctgcct tcgccgcaag cagatttaca accagatcga agcgggggcg
    11941 gaaagaccat ccgaatgctg tggccaaccg cagaatggag gggtgaaaac cgggggaacg
    12001 ggccgcaagc aagagccgct ctgcatcggc cttgctagcc gcgtaaagcg agaggggctt
    12061 taacgcagag cgctcatcga cggggctgct ggcagctcca tagacgctgc acgtggaagc
    12121 gaacaaaaag cggccggctt ctaaagcccg gcacacttcg gctaacagcc gggtggcggc
    12181 gacgttggtt tgaagcgtct gattttcatc aaaggcgcac gccatgtcgc cgacaatccc
    12241 gcccaggtga atcacggcat ctacgcctct gactgcccgt acgacgtcct caacgcggcg
    12301 aaaatcgcct cgcctcaacg caaagcgggg atgagatgcg aggtcctgga gagattcttc
    12361 gccgtaaatc agggagtcca ggacccgcac ctcatagccg tcggccagaa gatcccgaac
    12421 cagcacgctg ccgatgtagc cggctccgcc gacaaccagt attctactgg cggcctggat
    12481 cactgtttgt ttagggcctg aatgtgttag gaatgcagtg ctcataggtg ttacttcagt
    12541 ccgccggcgt tcccacgggc cggagacgac cgccgcaatg tgtagccgcc gttggcggat
    12601 gcctttcccg attccatctg ccagccggtt cctgttgatt catgctcttc cgccgccagg
    12661 gcctggccgc aggctggccg ctccattcga taaattcgaa gagtcagcga gcttccttcg
    12721 tagttctgcc tacgccatat gccgtgtgcc gcctacccct ttcatgttga gaatcggaag
    12781 attccccgga gaacttcaga aaaattcagg aagacgcgcc ggcggcgctt tcggccggcg
    12841 cagagcggga acatggattt gccggtatgt gctacttcgg ccggagccgg cgctggtaga
    12901 tcagaacccc gtagtcgtct tctgttcctc cgacaaatcg gccgaattcg cccaccactt
    12961 cgtaattctg ttcaatccag tgataaacag tgcggcagta atcgacgccg aaatcaggca
    13021 gaccatactc gccggttttg cggttggaga gcaagatata atcaatatct tctttctcca
    13081 gttgggccac atactcgcgt tcccggccct cgggctccag cacgcccggt tgaagcccgt
    13141 accagcgggt gggcgccagc gtgtcggtaa aaaaataaag ggaaatctcc tgcggcaaaa
    13201 caacgacgcg ttggccggcc tctttctttt gctccataaa cgacaggagt tgtctataaa
    13261 ccgccgattc cgccggcgtc ccatagatat cgccatgctt ggtacgaacc agcgccggat
    13321 ggacgccagg gtccggtaaa agagtgagga acagaccaaa ggcctctgcg gccaatacac
    13381 agcggcgaac ggagaggctc cgcaaagttt tcaaatcttc acaagcgccc gccgtgatcc
    13441 ggactaagac aacgagaaag acgacaaaaa gcgcctggct acaataaaca ccatagtaaa
    13501 tgggaaggac cagaaaggcg atcctgacac ctatcagcaa agcataaaaa caaaccacgg
    13561 cgacgctcag gcgccggcgg tcgaaccggt tgcgccgcca ctgaaacagc gatagaaaca
    13621 gcgccccgac agcaagccag gtcattccgg aaggaaacat cagcagcggg aggccgtaga
    13681 ggtaaagagg accgctgacc tgccgcccaa agagccgcaa tgcggcgcag gcggtgaaca
    13741 ggctggcgcc gagggccggc agagcccatc tgcgagcgga taactgtcga aagaagaaag
    13801 cgacaacaaa ccagccggcc atcgctagta cggccgtcac cattgagctg acgatgtctt
    13861 gcaggtcgag gcgcagcccg tagcggcgaa tccattgtgc tccgactgcg cgcatgtgat
    13921 aggaaaaggg agaattcagg ttttccaaaa ataaaaaaga cggtgatagc cgccaggcaa
    13981 accatccata agcgccggca ttgaccagca atcccggaag acaaaccaag aagcccgacg
    14041 caagcttccg agcggagcgt ccggatatgg cgtcggcgga gatgagaaac gccaccgtga
    14101 gataggatgc tgctccgaac tcctgtttcg tgagtagagc caaggcggcg caggcgctgg
    14161 ctccgcataa attgcgcccc ggctctccta agctgtggcg aacgcaaaaa tagagcgcca
    14221 gcaaactgaa gacggatgcc agggtcgtgg cataggcata agggagaacg aagttaaaga
    14281 ggtcgatttg aaacgcctgt agcaagaagc agccggcggc caggaaagcg gcgccgaggg
    14341 gaagcagccg ccggctgatg gcaaacagcg tcaaggccac aagcagcgtc tgagtcagac
    14401 cccagagata gaggacacgg agttgagttc caaacaggta aaaaagcagc gcattcgagt
    14461 acggcgccaa ggggccatac gtataccaca agtcccggta cagcattttg ccccggagga
    14521 tcgcataggg cacgtacatt tcccgcccgg tgtcgtacct agaaaactct ccccagtgac
    14581 cccatgtgag ccacgcgaag ataaaaaaga cagccaatat ccccgcatat gccatccgtg
    14641 gcgtagactg tacggccgcc gcctgccact tgtagcacag gctgatccat ttccggtttt
    14701 ggatagttca cctccccgtg gccgcagagc aaaaacagat cgtagcagcc tccgggcggc
    14761 ggcgggcaac gataagaacc tgcaacccag agggggcggc agcgaccggc tgtggcgaaa
    14821 cggaaatgtg aaggagaacg aaccgggcct gtagggtcgc agccctcgcg cgcagcttgc
    14881 gagcgccggc taacgcggca acgattctgt ggacgggggc ttgtgcggcg attccggcga
    14941 ccaacgataa ttacgcagcg tgcgggtaat ttcgttgtcc ttccagtccc aaagccgggc
    15001 gtgatcgatg tccacgtctg tcggatccgc gtaccagttt cccgtccaga gcatcgcccc
    15061 cgcggtttga acgaagatgc tcgcggccgc gagaacttga aaagtcacgc gacgcaaccg
    15121 cttcgcgtca atttcaggaa ttacaggcgc gatgagcaag accagatacg gacaggcctc
    15181 gctgagcaag cggggtccat agcacatccc cccccaccag gaggaatagc agctaaaaag
    15241 gagcacctgg ccgaaaaccg agagggccag cggttgccat tctcgccacc ggctgtcacg
    15301 cagaacgcgg atgcctcccc agaatccggc aagcagaaag ggcgagaaga taaacaaacc
    15361 gcggcttgga ctgatcaaca ggcccgccag ccccgttgga attgcgctgg tccacacgga
    15421 gggcaatcgt tgatgttgca acatatccgc gttgacctcc cggtatccgc cggagaggct
    15481 gttgtaaaaa tggagattaa aagcgagtaa cagtgcggct accaccgccg gcacggcgag
    15541 aaagcgcggc aattcgcgag ggcggcgcat ggccacatac atcgtgaaga ttccggcagc
    15601 caccatcatg acgggccgcg ccgcaacggc cagactgagc atggcgccgg cggcgatcag
    15661 ccgctttggc cggcagagcc acaggtaagc cgcaatcgaa aggaacagca tggcgggccc
    15721 gtgcatccac aacgcttggc tcgctatagc gaaagcggat gtcccaaacg catataaatg
    15781 cgccaaggcg aacgccttcg actgtgaggc ataagccgcc agcgtcagaa agacaaaaac
    15841 ggcagagcag gccgcgatca acgacgcagc cagcttggcc gagaatatac acatttctga
    15901 aaagatcggg ctgccgtagg aaattccctc ggccttcagc cagagcacgg gccccagata
    15961 aacggggacc gccatgattg ccggcagaac gggatacgag gatatcaccc ggccgtttcg
    16021 aaattgcaaa aaatagggaa gtggtctctg gtttatcctc ggtaatgtcg ggaaacgatc
    16081 gagataaaaa tcgccgtgca ggataatgct cacgggcaga tacatgttcg gcactgtatc
    16141 ggatgtgccc aggtagtgtc gcgtgaaatt atagagaaat atgcaggaaa aaagcagcag
    16201 acaggccagt cgctttctgc ggcgcggatc gggaacggct ccacccggat gcgcggcaga
    16261 tcccgcagtc attgcacggc tgttttccat gatttagcgg gaagaaaggc tgatcctgca
    16321 aggcaaacca gaaccatcag aggcgtcagt aggaggaagc ggtcgccgcg aaccataaat
    16381 attggcgtca cgggaaacag aacgaccagg ctgctgaaca ggcaggccgg gttaagccgc
    16441 gcgagcaaaa gcaaagcagg caaagccagc agataatcgg aaataaagac atgcggcaca
    16501 atgagcagcg accccaacca cgcggcggaa actgcctcga gttgcggcag ccggcgggcg
    16561 gctgttatga cggccgccgc cacagacagt attaggggaa cctgcaccca ggtattggca
    16621 acgaggctcg cgacgttcgc cgtccgttcg gggaactcca tttgcggcgg agtgcgctgg
    16681 atcagcattc gcgcgtactc aatcatcccg gccggcccca ccaggaaggt cgagatgagc
    16741 aagagaaccg atgagccgac ggcgaaccct gccagcgcac gccagttctt cctgatgaga
    16801 aaacacagcg ggaccaggag caggagattg aacttgatct cgcacaggga caacgctaag
    16861 ccggcgctga aagggcgatc ccgggccagc aataagtaag cggcggccgc cagcagaagc
    16921 acaagcggca catcctggcg gttgaagacg gcgtagaggg cagggaaaaa gatagcagta
    16981 tgccagctca gtgcggaaaa ttccatgttc atagcccggg cgctcaagaa caaagcgaca
    17041 accagcgcta ggatattcag actcaaccaa accagcagcg cccggccggg agacagcaaa
    17101 ctgaacggcg acaaaacaag ggcgtaaaaa ggcgggcgga taaacggcga agtcctgcgc
    17161 tcctccggga acagacgcat ctgttgttct cgaatgacag cccagtcata gagcgcgtgc
    17221 gggccttcgg cgcgcaggat ttttccggcg cagtaaaaag gcgcaaaatc cgcagccacc
    17281 ttccggacag cagactgcca gaagacgaag ccgatccata tcgcggctat tagggttgca
    17341 gcggtgaact ccaggacgcg ctgcccgcgc cgcgccgccg gagtcagaga tgactgtttc
    17401 aagactaccg gtaatgacgc cgccccagac atgagtcgag ctccaacgat tctcgaaaaa
    17461 ccgagcgcgc tggcgcctca gccggcgcga ggcccgcgcc ttaccagtcg aggtgaaagc
    17521 gctctattct tcccgcttac tgcgccggcg tatgatgatg atacccagca aaatcaccgc
    17581 caatacagcg cagacaattc ttacggttgt tgtgtccatt cttcctccgc atcgaggtag
    17641 tctacactac tttgcagcga ggccgcatca ttggaccgtc ttcatccacg ggaaaaactc
    17701 gaagatcagt tctaataatg ccggacctac cgtcaccaaa aacatcgacg gaagaatgaa
    17761 aaagaaaata gggaagacca gcttgacgcc gactttattt gctttttcct ctgcgatctg
    17821 gcggcgcttg acgcgcataa actcagcgtg gctgcgcagc gcctgagcga tggaggtccc
    17881 gaaacgatcg gtttgtatca ggatgctgac gagcttcttg aactcgtcct ctccggtccg
    17941 gtcagccagg ttccgcaagg cctcggagcg cctcttgccc gctttaagct ccagggtaac
    18001 cagggaaaat tcatcactga gctgaggatg ggtgatactc agctctttgc tcacatccag
    18061 tatggcctga tcgagaccca gtcccgattc cacgcaaacc acaagcaaat ccagggcatc
    18121 cgggagggag tgccgaagaa tccgccgccg gcgctgtatt aacatcagca gaatctcgtt
    18181 tggaagcgca taccccagca ggccagcgag aagcgacagc gccaatatca tctggcccga
    18241 aagcaagtgg gtaatcgcca gcccgatggc tgaaagcaaa gccaaacccg ccgcgcagag
    18301 cacgcgcaaa ccggaaaaaa ccgccaccgc ggattcggaa cgatagccgg cggtgatcaa
    18361 acgacgccgg agtctccgca cggtagaggg agatacagga acggcctcac cgagactctg
    18421 tacgagcttc gtgaccccca gttcatcggg tccgtcttcg atgtaatccg cgagatgatc
    18481 gaaggtctgg gcgcccaccc ggtccatgta ggccgcgggt ttcacccaat agcggtaccc
    18541 cacaaggctg atcactccgg ccaccagtac gaaccacact agccccaaga tcagcaaaga
    18601 catgattaca cctcgatatt cacaatcttg cggataaaca aaaaggcgat gccctgcatg
    18661 ataatggctg cgccgatcag gtgctttcca atgggaaact tgaccaaggc caccatgtat
    18721 tcggggttca ctaccatcat gagcagcaca acgaccaccg gcagcacggt caggaccttt
    18781 cccgtgagcc ggccatgggc gcttagagcc ctgacctgac ctctcaactt gaaccgttct
    18841 cgaatcacct gcgccagctt caggagaatt tcgctcagat tcccgccggt cctggcttgg
    18901 agcatcacgg ccgacacaaa gaagcgaacg tcgagcagcg gcacccggac ggcaaggttt
    18961 tgcagggcgt tttccagcgg caacccgagg ttgtgctcat caaacgtgcg gcgaaactcg
    19021 gtagacactg ggtccatggc ctcctcggag agcagttcca ggctgatgga gaaggcatgg
    19081 ccggcgcgca tggcccgcga tagaaagtca agcgcttcgg gaaactgctc ctcgaatttc
    19141 gccagccgtt tgctccgctt ccgcatcacg tagccgtaag ggatcatccc ggccagcagc
    19201 acggcgggcg ggatcagata gccagggaac cacgaaaggc ggatcaaaat attcaacgcc
    19261 aggaaggccg tcacgagcat catggcggtc aacagcccga ccgaccactt caggtttgcc
    19321 tgggcaaggt attgctgcaa ggttctggta aaggcaaact tctccaatag ctggccgtaa
    19381 aaaggcagta tgctcgtcgt atccttcttg agcagatccg ggtccgcctc ctcatcctgc
    19441 tcgattgtgc gctggagccg ggagtccaga gctttttggc gccgcttctc tacgacgttc
    19501 catgccgccg aagcagcgat cgcggaaacc gcgaagatca tcagaaacgc cgtgaccaaa
    19561 atgatcgtcg gtaacatgat ctacctcatt gccgcctttc tgcggcgtca ctgaatgtgg
    19621 gtgatcgtct cgaacatggc cggtggaagc tgaatgcccg aggctttgag cttttgcgcg
    19681 aacttgggac gaatgccggt cgcccggaac tgcccgatca cgcgccgctc gttggtcaag
    19741 cccagccggt caaaaacgaa cacgtcttgc agggtgatcg tgtcctcttc catgccgacg
    19801 cattcggtga tgtgcgtgac gcgccgcgtc ccgtcgctca gccgggcgat ttgcacaaac
    19861 agatcgacgg ccgagctgat ttgctggcgg acgctcttcg gagaaatgtt cgagttcgcc
    19921 atgccgacca tcgtttccag gcggctgatg gcatcccggc aactgttggc gtgaattgtg
    19981 gtcagggagc cgtcgtggcc ggtgttcatg gcctggagca tgtccagcgc ttcctcgccg
    20041 cgcacctcgc cgacgatgat tcggtcgggc cgcatgcgga gcgcattgat caccaactgc
    20101 ctttggcgca cagccccctg gccttcgata ttcggcggcc gcgtctccag gcgcgccacg
    20161 tggatctgct tcaactgcaa ctcggcggcg tcttcgatgg tgacgattcg ttcatccacc
    20221 ccgataaacg ccgaaagggc gttcagcagc gtggtcttgc cggcgcccgt tccgccggat
    20281 atgacgatgt tcagccgggc ctgaatgcat cctttgagca actggagcat gtctttggtg
    20341 agggtcagat tcttaaccag atcctcgtgc gtcagaggat cgcgcccgaa gcggcggatt
    20401 gacagaagcg ggccgtccac ggccagcgga ggtatcacgg cgtttacgcg cgatccatcc
    20461 gggaggcggg cgtctaccat aggagacgat tcgtcgatgc gccgcccgac ccgcgagacg
    20521 atcttctcga tgatgcggag caagtgagcg tcatctttaa actgcacggg cgtcaaatac
    20581 aacttcccgg cgcgctcgat atgcaccagg cggggcgttg tcaccaggat gtcgctgata
    20641 gtcgggtcct gaagcagcgg ctcgagcggt ccgaggccga aaacctcatc gagcacttcc
    20701 tcgatcagtt gctccttttc gaccaggctc aaaggagttt tttcttcctc gaccaggccg
    20761 gtgacggccg cgcgaacttc gttgcgcatg cgctggccgt tgatggaact gagtttttcc
    20821 agattgattt tgtccaataa cttgcggtgc agggcgaact ttcgctccag atgctccgcc
    20881 gaagggttgc tgcggagcag caaccggtct tcccagctag tgcctgatgg tgaatgaaat
    20941 gtgcttgtgg ccatagattt tctcgacccc cttcacgccg gcgtctcgcc ggcgctcttc
    21001 tacaatccga gcaaagacca cttcttgccc gcctccggca aggagggcgg tccggagatc
    21061 ttggcgacga atttcgtgat tgattttccg aggtccgaat tcggagaaat cacgccgcct
    21121 ttgacataaa attccgtcac gctttttgca tcgttcggaa agcgccaaaa gaccggtact
    21181 cccaggttgc gctcgatgtc ttccgggagc acttcatcgt gccgcgaact ccggttcacg
    21241 accagcgcca cgcggtcgag cggatgcccg gccgcattca atttctgcaa cgccctcttc
    21301 gcaaggtgca agcaaggcag atctgttgtg gtgatcagaa agatcttgtc cgcatcttcg
    21361 agcgccgcca gggtcaagcg gctcaagctg ctcggcatat ccaggacgct gtaagcgtaa
    21421 atggttcggg caaagcccaa aacctggcgg acttcaacgc cctggggatt atcgaacccg
    21481 cgcagatccg acggcgcagt caggatgttg aggttcggct ggccgttgga aataagcccc
    21541 ttccagtagt gcacgtcaag ccggctgatg ttccggcaag cgtcaagaat gctgtactgg
    21601 gatccggctt tcatggcgaa cgccacatta cccgtctcca gatccaggtc ggccagcaag
    21661 atttcctgtt tggtggatct tcccagcgcg gcagccacat tgcacgcgag cgtcgttgcg
    21721 ccagcgccgc cttttaccga aacaaacgcc aggaggcgcc cgcccgcggc tttagagccg
    21781 gaagcgcccg gcgcaatcgc tgaaatacgt tccagggcgt tgaccagtgc gcctggctcg
    21841 agcggcatgg ttaaaaactc cgccgctccc gcccgcatcg ccttcagaat gaccccggcg
    21901 tcgctcgaat cgtgaaccac gaccacggcc ggcggatggc tcatcgtgcg catgagctgg
    21961 atgtacgaga aacgctcatc gggctgttcg cccagatccg ctaaaatcac atccggctgg
    22021 agccgttcga ttcgctcgtg caccagatgc caggcgccaa agcccccctg gtcgagaacg
    22081 acgcggacag ggatctcttt tagctcctgg aggacctgct cccgaaagct cggccgttcg
    22141 agaatcagcg ctaggctcaa cggtttcaac ggttcgcccc ctgtgcgccg gccccaccct
    22201 ttacccgcgg attctccgcc gggtgctggc cgggaatttc aagattcggc cggtccatct
    22261 tgggcggcgg aggggcttct ccctcattga acggccggac aaactcgggc gtcaccagca
    22321 ccatcagctc ggtgttgctc cgggtgagcg acttgctgcg gaacaggttc ttaatgatcg
    22381 gtatgtcgcc cagcccggga atcttggaga agctttcaat cacccggttg tccagcagcc
    22441 cgccgataat aaagctttgg ccgtcggcca gttccacctc cgaatccagt tttcgcgtgt
    22501 tgagagcggg gatcgtgaac ccctgaaagg tcagggcgtt cgtaaaatcg agcgaactca
    22561 cttcaggccg cacccgcagc cggatcgtat tgcgcggggt aatgactggc agaaagccga
    22621 tccgcacgcc gaattcgcgg aactgaatag ttaccgcgcc cgccacggct ccgccctgca
    22681 ccaccgggaa cgggaactcg ccgccggcca aaaacgtcgc ttcgcggttg ttaacggtca
    22741 tcaggttcgg ttcggcaagg atctgaagca gcctcttctg ctcgagagcg tgaatcgtcg
    22801 cggcgatgtt caggtcgggc cggaaggcga agatgttcat cagatccgtc acggtaaaag
    22861 ggacctgcgc cgcgttggct ccgccgatct ggttgcgctg aagcccgccc tggaactggc
    22921 ctgtggttgt cgagccgatg gtgttcagcg ccccggtgct gataatattc gcgcccagtt
    22981 cctgaaccgc cgcccggtcc acgtcggcga accggacctt cagcataatc tggggctccg
    23041 ccggcggagc ctcgacctgt agcaagttca cgacctttcc cgccgtggcc gcgatcgcct
    23101 gcgcccgctc ggccgcttgc gtgtcgcgca ccgttccacg cagaaaccat gtattgtttt
    23161 cgtagctcaa gtgcacgttg ccatcgggaa aagattcctg aatctgctgc gccagcggct
    23221 gcaaatccgg acgcaccgtc aggttgaaga aaatgcgccc gccgccttcc tgccaaagaa
    23281 taaggctagt ggcgcccgcc gcgcgcccgt tgatgagcac ttcgtgggga tcgacgatga
    23341 tcgcgtccgc cacttcgggc gtggcgaccg agacacgttg aatgatcgga gtggaatcaa
    23401 tcatcaccga cttgccgacc agaacggaaa gctcccgcgt agcctgaggg gccggcgcgg
    23461 cctggccgaa acacaaggcc gcgcaagccg tccagaccag ggcggctttc caccagaatt
    23521 tccatgagcc caaccgctta gccttcagtg tcaaaccgcg ctccttcttc gggcgtttcc
    23581 gtgcgctgcg ctattgcagc ttctcacccg gagtatcctc tgtcttgaat ttctcgcttg
    23641 tccggctcgg ccccttcaac agctccacga caatcggcgg cggtggcggc ggagggggcg
    23701 gagtcgccgg cgccaccatc ttcgtcacta ccttccgcgg cttcggggga gccggcgtat
    23761 ccgagggcgc cggccccgta cggaacaagc gagccaccgc tgtacctttg gtttccgcct
    23821 tgttggaatc caaaggattg cggagcacaa gctgaatccg cgtctcgttg ctggccaagc
    23881 tcagaacctc agcttcttcc ggagtgacca gcagcgtcac cacgggaacc tgcaccggct
    23941 tgccctccgc gtcgtgctgg atgttggttc ccgccgaaag cacttccacg ttctggagaa
    24001 cggttcggat ctcgggaccc tgctgattct ggcccggagc gttgccggtg accaggacat
    24061 ccacccggga cccgggcagc acgtagccgg cgacgcccac tacttcgttc acgcgcaccg
    24121 agaccgcccg cttcccgatc ggaatagcag gcgccaggcc ggcccctgct tcccttggcg
    24181 caagatgaga ctcgagcacc ggttcgcctg ttgcgacttc ggctaggacg cccctgccca
    24241 ccacgttcga tttgtcggtg aaggacccct tcggcaggat cgacgtaccc catcgaacca
    24301 cgccgatatc cgaatccttc agaagttcgc cgcggtgcaa atcgcgcgat gccaccacga
    24361 tctgctgggc aggcgcggcc ggagcgccgc tgtgcagccg gctggaaatc aggctgtaga
    24421 gcagcagcgt ggccgccgcc gctacacaaa gagaaaatcc gatcaccgtc aataggcgtt
    24481 tgttcatgag acacccttat tcccagcgcc gctcatttgg cggcgctggc cgtgttggaa
    24541 agatggttgc tggcgatttg ccagatagtc ttcagattgt tggcaaaaac cgggaagatg
    24601 gctgccgccg ccagcgcgac aaaacccagc aaaagggtgt actccatcgt gtcctgtcca
    24661 tcttgttctt gtgtaaacct tcccagaagt tcccgcatcc cgctctctcc caatgaaatt
    24721 ctgcgcggct ttgtcgcaat ccgcgctttt tgattcaagc cgcaggcatg gaggttgggg
    24781 gggcctccat gcgcccggag cgctctactt tgcggcactg gctgcgttcg aaagatggtt
    24841 gctcgcaatt tgccagatag tcttgatatt gctggtcacg aacgggaaga tggccgccgc
    24901 cgccagggca acgaacccca gcaacagcgt gtattccatc gtatcctgcc cgtcctggtg
    24961 tttccagaaa ctgcatagta tgttcttggt ttttcgcata gactcaatct ccttcggatt
    25021 tgtgatcgct tcgctcattc cgcctccctt ttccagaggc gactcttact caggcgcaac
    25081 ggctgcctcg cagaagcgcg gagaggcaga gagcatttca atctctgctt ctctgcgcct
    25141 ctgcgattcg ctactgcgcg gcactggctg catttgagag gtggttgctc gcaatttgcc
    25201 agatggtctt gatgttgctg gttacgaacg ggaagatggc cgccgccgcc agcgcaacga
    25261 accccagcaa cagcgtgtat tccatcgtgt cctgcccgtc ttctcctcgc cagaaattcc
    25321 tggcaagaaa cttgagatat gtcatgatta cctccaaaaa aacgcgccgg ttgtttgcag
    25381 cgcttccgcc cggcgttgat ttcagttgtt tctgggcggg cttactgctc ccgccggcct
    25441 gcgccgcgca cggaggccac caatcgggcc ggcgcgcttg cggcacgttc aaaaacaata
    25501 gcgatcccgt cgtcgtaggc gactttccag tgaggcgact ccttcaacgc gcccaccagg
    25561 ccggaatcca ccggcagcac gacggtttcg actccgtatt gctggaaagt gcgctcccag
    25621 gcgtggtgca cgttgatgag gtccagatac agctcgccga gcttcggacc gtaataatcg
    25681 ctgcgcccgt ccatgaacac ccggacgcgc ggaaactcgc gataaatcaa atagctcgac
    25741 cactgatcgg tggaaaacac gttgcccgag aaagaacttc tttccagaaa gtccgccgcc
    25801 gccacgggga acagtttcgg gttgaaccgg ggatgaaaat cctgcaaacc ggcagacgaa
    25861 gccggggacc acagcattgc cacaaccagg agcgtgacca tggcggaagc cgcatagacc
    25921 cgcggcgccc ggtccatcct ggcgatgtcc tcgccgaaac gcccgaacgc gcccgccagc
    25981 ccgcgagccc aggctgccca ggacgcagtg tggtgcggag ccagcgagtc ggtcagcgcg
    26041 gcagcgatca gcggtgcggc cagaatggca taaagcggga cgtgccgcgc ggactgaaga
    26101 ctgaagtggg cgaaactgac caccagcagg gcctcggtat agcgcccttg ccgccaggcc
    26161 cagagcgcgc tcgcaattcc gcctagcaga aggatttcaa agaagcgttc cggcgcggcg
    26221 tggaaattgg gcgaaaagaa ttcggtaatg tggtccagaa tgtaggcgtt cttcagatag
    26281 ccgaagatgt ggacatgaac cttatagaag taaggattca ccaggctggc cgctccggag
    26341 accgccaaca gcagcgcgta gcggcccgcc cgccgcagcg ccccgcggcg tattgccgcc
    26401 gtgtcttctc ccgcagccag cgcggcttgc accagttcgc cggccccgta gagcgcgatc
    26461 aaaaacagac caacgacaaa actaccgtgt aagttcgtcc atagtatggt caaaggcgcc
    26521 agccagaaaa tcgcgtttcg ctcgaccggc gcctcttcca gcacccagta gaaaatcaac
    26581 gcgaacgccc aactgaaaat gtgcgggcgg gcgagccagt ggatcgaggc ggcgtaagag
    26641 gcggtgaccg ccagggcggt ggccagcagc acgttggcgc cgcgccggac catgaatcgg
    26701 tacaggaacg taaacaccag cgcgatcacc agccccgcca gcaggaccaa gcctgccagg
    26761 cccgcataac ggtgtacaag ggcgaacagg acatcgctca gccattccca ggcgaaccac
    26821 tcgccgcccg gcttggagaa ggaaaacaaa tcgtgccggg gaaccgcgtg attttggaga
    26881 atccactcgc cggtgcggat atgccagccc gtgtcggaat cgcccagcag cgtcgtcgct
    26941 cctttgccga aaaggaaaag gaacgccagc gtcgtcgaga aaatcatgtc gctcaacgaa
    27001 ggcaacaggc tgcggcgggc tgtggcgttc tgcggcatat cagcgaatca gggagacgag
    27061 agtctgtaaa gccggcgtct tgggcgaaac cagggctatg gttgcattac cgttgaggct
    27121 catggcccct gtgatgatca gagtcggctg gcttgtcgca ccgccgccct gcatgttcat
    27181 cagcgagcct tgcggcatgt aaatcatccc gttgaacccg gcgggcggcg atccttgcgc
    27241 atcccaggtg gctgtgttct gtgttgggca aggcgctaga aaagtggcag tgggacagac
    27301 cgatccaccg ggcgtgggcc agggaaggcc tgcggaatta atagcgctgt ttgcgagcgg
    27361 ggtgttgcgc cggtccaccc acacaatcgc cggctggtac aggtaattcg agttattcag
    27421 tggattcgtg gccggagact cgcatacgcc cggatcgcag gccggataat tctccagccc
    27481 cgtgatgttg accgatagat ttcccttgcc cgaattcgac tccgaaattc cgccaaagcc
    27541 ccagttggcg accgccgccc acggcgtgcc cggcgatggt tgtactgccg gaacgcccgc
    27601 ctgaaccgta aaaaggctcg tcatggcctg aatcggagga ctataggcgg gcggcaaaag
    27661 cttcacggca ttcgatcccg atccgtccgg cgtgtagtcg ggacgggtga gcaaaagcat
    27721 ttctccccgg tagctgccgg gcccaggacc gctgacgccg tctgtcaagt tgagctgact
    27781 agatccctgc aagctgagta tgggattgct ggcattagag acgccggcga tgacatatgt
    27841 gccctggtcg agcttgacgt ttccagcgag gttgaggcca ccgaaaaaga tgtaggtccc
    27901 cggtttgccg cccgagttaa atgtcacatt ggtgagagtg atcggcgctc ccgaaatgtc
    27961 gaacagttta cttgacggat tgtacgtgcc gcctatgtaa gcgccgggcg tcagcgtcgt
    28021 gttgctaaac cctcccggga cgccgtagac aacctgccct ggcgtattat tgacgacttg
    28081 ctgtaaagtc gggaccgtgg gttgattccc ccggttcatc gagagggttg ggtcgggcac
    28141 ttgaggaacg ttgatgaact ttacaccgct cgaactgggc gccactttcc cctggacgct
    28201 tccgtcttgg gcaatttcca gcag
//
LOCUS       DASREK010000135         9070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_896282, whole genome shotgun sequence.
ACCESSION   DASREK010000135 DASREK010000000
VERSION     DASREK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896282"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..959
                     /locus_tag="VFA54_06715"
     CDS             351..959
                     /locus_tag="VFA54_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS50531.1"
                     /translation="MDSRTELPEHQGPGADPDAPLVARCLRGDAVAWEELVKLHTRRV
                     YAICYRFTGGHDLARDLTQEVFLRVFRNLGNFRAGSGSFQVWLASLARNLLIDHYRKT
                     RKDKLLESIEERLPELEESSIPSGRTDGRLASREAAEFLQAGLERLSPELREAVILRD
                     LEEMEYREIAEVLKVPEGTVKSRISRGRAELARVLRKLGVAL"
     gene            1040..1810
                     /locus_tag="VFA54_06720"
     CDS             1040..1810
                     /locus_tag="VFA54_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZS50532.1"
                     /translation="MQCSEFEIRLCDYLDGSLPTGQRQAHEEHAAGCARCAALLEDCR
                     AVGRFLERVDAVEAPPELVTSILHQTRSGSWALLHPGWGASRAGEEARGWRSWLHPLL
                     QPRYAMSMAMTIISLSMLYRITGAPIRQLEASDLSPVAIWYRIDDSAHRIWNRGVKLY
                     QNARFVYEIVEEWRTLESEEEPPAPAEAAPGPDSGSGAAPGRERPVEPRSPRKVDPRN
                     GNPAAPESGASEPGEARGSRLLRNSGQPAGRIMGTANL"
     gene            1807..2493
                     /locus_tag="VFA54_06725"
     CDS             1807..2493
                     /locus_tag="VFA54_06725"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZS50533.1"
                     /translation="MNCATHPEAPAVAYCRNCGKALCNECRQEWQGVIYCNSCLAQQG
                     ATPRPPSPPLPAPPAGAPSPAAAFILGLIPGVGAIYNGQYAKGILHIVVLGLLISIAN
                     SDAAGELQPVVGMLIAGWFFYMAFEAYHTAKRRLHGEPVDEFSGLLRGSQKQGGIPIG
                     PILLVALGVVFLLNTLGYLAWDRIVRFWPVLLIAAGLYSLYARLAGRSEREDRSGPAG
                     SGSGRLESKE"
     gene            2500..2700
                     /locus_tag="VFA54_06730"
     CDS             2500..2700
                     /locus_tag="VFA54_06730"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZS50534.1"
                     /translation="MAAYFCGCARCTAGAITGPLMLITLGGLFAIDQFGRYRFHQTWP
                     VLLILYGLMRAVAYMMPSHGDG"
     gene            2724..4115
                     /locus_tag="VFA54_06735"
     CDS             2724..4115
                     /locus_tag="VFA54_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZS50535.1"
                     /translation="MATSNPAPARRGSLVAPLMLLGLGVLFLITNLRPEISVWRLFAQ
                     YWPFLLIFWGAARLVEYTIARAASRPIARTLTGGEILLAVAICLVGHVMFVAQRGDWG
                     VWRFGDRGVEILGESYDFPLPPSNIELPARSEVAIENLQGTIRVSGTDSRTMRIAGRK
                     YIRAFDRAGAEQADKASGISVRKDGGRVQVQAGQGSLSGSRHISCELEITVPRDSILR
                     LEGRNGDIEIRDMTASVDVTGANSPVRLSNIGGNVRVEVRRSETVQAEQLGSNIEVTG
                     RGRDLDLTKIAGTVTINGDFSGNIRLAELARPARYTSSVTDLSFQKLPGKLEMDLGVL
                     YAVNIIGPLKIQTRSKDVRLENFSKDVDISSRRGDIDLRDPTLPLASIHAETQNGDVS
                     VALPSNAVFSLQAETAHGQIVNQFGAAVRVEEHGRSAQAHGGVARGEGAEIRLRTGRG
                     SITLAKTGDVQAL"
     gene            4237..4860
                     /locus_tag="VFA54_06740"
     CDS             4237..4860
                     /locus_tag="VFA54_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZS50536.1"
                     /translation="MLVDYEVVTRARSGDAAAFNELVSAYRKRVLGTVFRLIGRQEDV
                     EDVGQEVFLRLYYSLDQLRTAEVFEPWLYRLTVNAAYDYLRKQRRQREARMADLTEEQ
                     VLAADAAVGGKQALEEDQKAHIRELVQSLLGKVSEEDRILLVLKEVQGLSLKELERIY
                     RVKENALKVRLFRARQRVLKAFEGIQKQELKIKTLATGGKHVGRKAV"
     gene            4838..5176
                     /locus_tag="VFA54_06745"
     CDS             4838..5176
                     /locus_tag="VFA54_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50537.1"
                     /translation="MLDEKRSDGLERLWEAYRLATPEPAVSVNFMPQLWARIEAARPT
                     SWTMPLTRLASRLVPLAAALTLAMSAYLWIPQSRTNANSQTGYVDVLASDLLEQQRPA
                     LWVANAEDSI"
     gene            5177..5578
                     /locus_tag="VFA54_06750"
     CDS             5177..5578
                     /locus_tag="VFA54_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50538.1"
                     /translation="MTKPTRIAALCLGIVFLAGAIFGFVAHGLYAEHTTRASNPQQMR
                     ERYISNLQKELALSPGQVAQITSILDETRQRFREIRERMEPEFESLRELQRERIMGIL
                     TPEQQPKYQAILEQHRREQERRRQQNTKAGH"
     gene            complement(5822..6760)
                     /locus_tag="VFA54_06755"
     CDS             complement(5822..6760)
                     /locus_tag="VFA54_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS50539.1"
                     /translation="MPPTRVPFRAFAAFLCAAAIFQAAALAQNPAPQSPLLPENSVTR
                     VSEHVYAILGFPNIGIVVGSRATLVVDTGMGRRNGALVAREAAKLGKHPLLYLTTTHF
                     HPEHAAGEPGFPLETILIRPAVQQEEMEQRGSEFIEMFRGRSALNRELLQDVKLRRPD
                     VIFDREVKLDLGGATARLFWLGAAHTRGDELIFVEEDRTLLSGDIVQKNLVPGMPNAD
                     SSPKSWLAILDQIEPLQPRFIVPDHGQLGDGSLIAKQRTFMLDLQSRALELKRQGKSI
                     EEAAAAVTNELKAKYPEYTNINSVANVVRRVYAEAP"
     gene            7091..>9070
                     /locus_tag="VFA54_06760"
     CDS             7091..>9070
                     /locus_tag="VFA54_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50540.1"
                     /translation="MRSKAKTLVLWVAAVAAIAFFVPASPADPLSCAFGEYKPIPGLA
                     AQMAGDTLTLNWDGEGDQQLRLRLGINSGRPTMRELAVRHRGSPWIAAATNATPEFRV
                     VSGLRRITNQQLDPLAGLGVPITQEIIDKYKWEAFWDAPLNVPGGEGAHNNTTPPQRG
                     VLNQPGLPRSPDEIKRASAVYHVTACEVRTNGARLEISFPGVQLGVFAGRLQFTVYKG
                     ANLIRQEIIAKTEEPSVAYKYDAGIRGLAIQPGSRLVWKGLTNLWQEYRLGGPPNEGP
                     VVVKSSSRLLAAETAGAALAAFPPPHTFFWARETSYNLGYNWYQKESGSSFSFGIRQA
                     EREESAADENRGSEDRSQNFALLSARPGTWQRMPVYFYVSADSGPAAVEGALLYTRGD
                     RFKALPGYQVMATHFHMGLVARARRAGGLDALLPDLEMMQAAGINIVAPIDGGAGFAP
                     EGIGQTGRLRMAPMDDPKWFQWSRGLGAPAGAEEATGAADGASGSPPARASARSSTAR
                     RISGDLGSIDPYKSQADYYEAARRHSDRSFVVMPNTELMRGEVARTLGGHSDILISHP
                     VYWTQGRAPGQPLSEDHPKYGRVYHLGDTADMMEMIRRENMLIFMPHPRSKGSTGFPD
                     AIKDTPHFTDENYRGIGFRWGMGLDGSERRLCEYRC"
BASE COUNT         1717 a         2667 c         2938 g         1748 t
ORIGIN      
        1 tttgtctgga agtaccggcc gtgcgctctt cggctttccc aaagactggt tctccgacgg
       61 aaaaaatggt acgcgtgttc ccagcgacag gaccggatcg gccggttcgc gctggcaacc
      121 gtgaatttca agatatcagg aaaacagtga actttttaat gtacgctctc gttacctagg
      181 aggaaggatg aaccgcctcg ccgcgcggag cctaagctgg tgcgagaagc cctctcggcg
      241 cacagagtct gcgcagttca tcggctcggg cgcagtgcgg ggtgatagca gaacggcaaa
      301 tggtgggaac cctcgtttgg ccaaatgcgg ccggttgggg aacgatgccc atggattccc
      361 gcactgagct gccggagcac caggggcccg gggcggaccc tgatgcgccg ctggtggcga
      421 ggtgtctgcg aggcgatgcc gtcgcatggg aagaattggt caagctgcac acgcggcgcg
      481 tttacgccat ctgctatcgt tttaccggcg gccacgattt ggcccgcgac ctgacccagg
      541 aggtgtttct ccgtgttttc cgcaaccttg gaaacttccg tgccgggtcc ggcagctttc
      601 aggtttggct tgccagtttg gctcggaatt tattaatcga ccactaccgc aagacgcgga
      661 aggacaagtt gctggagtcg atcgaagagc ggctgcccga gttggaagaa tcatccatcc
      721 cgtcaggccg caccgacggc cgcctggcca gccgtgaagc cgccgaattt ttgcaagcag
      781 ggctcgaaag gctctctccc gaactgcggg aggcggtaat tcttcgggat cttgaagaga
      841 tggagtaccg agaaatcgcg gaagtcttaa aagtccccga gggcacggta aagtcgcgca
      901 tcagccgcgg gcgcgcggaa ctggcgcgcg tgcttcgcaa actaggagtg gcgttgtgac
      961 cactggcgat gtcgaaacat cgggcgccac ccgccgggca aatagctgcc cggagcgtcg
     1021 acgggagccc tgattggcta tgcagtgctc ggaattcgaa atccggctat gcgattatct
     1081 ggacggctct ctgccgacag ggcagcgtca ggcccacgag gagcatgccg ccggatgcgc
     1141 gcgctgcgct gcgctattgg aggactgccg ggcggtgggc cggttcttgg aacgggtgga
     1201 tgccgtggaa gcccctccgg aattggtaac cagcatcctg caccagaccc ggagcggttc
     1261 ctgggcgttg ttacatcccg gttggggcgc gagccgcgcc ggcgaggagg ctcgggggtg
     1321 gcggtcgtgg ctgcacccgc tgcttcagcc gcgttacgcc atgagcatgg caatgacgat
     1381 catttctctc tccatgctct accgtattac gggcgccccc atccggcaat tggaggcgag
     1441 cgatctcagc cccgttgcga tctggtaccg catcgacgac agcgcccatc gaatatggaa
     1501 tcgcggcgtg aaactttatc agaacgcccg cttcgtttat gaaatcgtgg aggagtggag
     1561 gacgctcgag agcgaagaag aaccgccggc gcccgcggaa gcggcccccg ggccggatag
     1621 cgggtcgggc gcggcgccgg gtcgggaacg gccggtggaa ccgcgaagtc cacggaaggt
     1681 ggatccccgc aacgggaacc cggctgcgcc tgaatccggc gcgagcgagc ccggcgaagc
     1741 cagaggatcc cggctgctcc ggaactccgg ccagcccgct ggccggatca tgggaacggc
     1801 gaatctatga actgtgcaac gcatcctgaa gcgccggcag tcgcctactg ccgaaattgc
     1861 ggcaaggctt tatgcaacga gtgccggcag gaatggcaag gcgtgattta ctgtaattcg
     1921 tgtctggccc agcagggagc cacgccccgg cccccgtcgc cgccgttgcc tgccccgccc
     1981 gcgggcgctc catccccggc tgcggcattc attctcggcc tgattccggg tgttggcgcc
     2041 atttataacg gccagtacgc caagggcatt cttcatatcg tcgttctggg cttactgatt
     2101 agcatcgcca attccgatgc agccggagag ttgcagccgg ttgtgggcat gctgatcgcc
     2161 ggttggtttt tttacatggc tttcgaagcc tatcacactg cgaaacgccg gttgcacggt
     2221 gagcccgtag acgaattttc cggactgctg cgcggctcgc agaaacaggg cggaatacca
     2281 atcgggccga tcctgctggt cgccctcggg gtcgtttttc tgctgaatac gcttggctac
     2341 cttgcctggg accggatcgt gcggttctgg cctgtcctgc tgatcgccgc gggcctttat
     2401 tccctctatg caaggctggc gggacgttcg gagcgggagg atcgatcggg tcccgcggga
     2461 tcaggctcgg ggaggctgga atccaaggag tgatgcggaa tggcggctta tttttgcggg
     2521 tgtgctcgat gtactgcggg agccatcacc ggcccgttga tgctgatcac gctgggcggc
     2581 ttgtttgcca tcgatcaatt cggccgctac aggtttcacc agacctggcc agtgctgctc
     2641 attctgtacg gattgatgcg tgccgtggcc tacatgatgc cgtcccacgg tgacggctag
     2701 atacgttttg gagaattaaa gggatggcaa catcgaatcc agcgcctgcg cggcgaggct
     2761 ccctggtagc tccgctgatg ctcctggggc tgggagtgct ttttctgatc accaatttga
     2821 ggcccgagat ctccgtctgg agattatttg cccagtactg gcccttcttg ttgatcttct
     2881 ggggcgcggc gcggctcgtg gaatacacga ttgcgagagc ggcgtcgcgc ccgatagcgc
     2941 ggactctcac cggcggagaa attcttctgg ctgtcgctat ttgcctggtt ggccacgtca
     3001 tgtttgtggc ccagcgcggc gattggggcg tgtggcggtt tggagaccgc ggcgtggaga
     3061 ttttgggcga gagttacgac ttccctttgc ctccttcgaa tatcgaattg ccggcccgct
     3121 cggaagtggc gatcgaaaac ctgcaaggga ccattcgcgt ttccggcacg gattcccgga
     3181 ccatgcggat cgcggggcgg aagtatatcc gtgcatttga tcgcgcagga gcggagcagg
     3241 cggataaagc aagcggcatt agtgtgcgca aggacggcgg ccgcgtccag gttcaggccg
     3301 gccagggttc cctctccggc tcccgccata tctcctgcga gctcgaaatc accgttccca
     3361 gagactccat cctgcggctg gaaggccgga atggggatat tgaaatccgc gacatgaccg
     3421 cttcggtgga tgtgacgggg gcgaacagcc ccgtgcgcct gagcaatatt ggcgggaacg
     3481 tcagggtgga ggtgcgaaga agcgagactg tgcaggctga acaattggga agtaatattg
     3541 aggtcaccgg ccggggacgc gacctcgatc tgaccaagat cgccggaacc gtaaccataa
     3601 acggggactt ctccggtaat attcggctgg ctgaactggc ccggccggcc cggtacacgt
     3661 cgagcgtaac cgatctgagt tttcaaaaac ttccgggaaa gctggaaatg gatttaggcg
     3721 tgctatacgc cgtcaacatc atcgggccgt tgaaaatcca gacccgctcc aaagacgtgc
     3781 ggctcgaaaa tttcagcaag gatgtggaca tttcctcccg ccgcggcgac atcgacctcc
     3841 gcgaccctac tttgccgctg gccagcattc acgccgagac ccagaatggc gatgtttcgg
     3901 tggctctgcc gtccaacgcg gtgttttccc tgcaagccga gacggctcac gggcaaatcg
     3961 tgaatcagtt tggagccgcc gtacgcgtcg aggagcacgg ccgctccgct caagcccacg
     4021 gaggcgtagc gcggggagag ggggccgaaa tccgcctccg caccgggcgc ggctccatca
     4081 ccctggccaa aaccggcgat gtgcaagcgc tgtaacctgt tgcacgcctc ggacgcctca
     4141 tcttcggagg ctgaggcggg cttttctcca ggccagcagt tgcgatacga taccggtgag
     4201 ccgcaggcac gatagccggt tcgggctgaa acgcaaatgc tggtcgatta cgaggtcgta
     4261 acgcgggccc gatccggaga tgcagcggct ttcaacgagc ttgtttccgc ctaccggaag
     4321 agggtcctcg gaacggtctt ccggttgatt ggccggcagg aggatgtgga ggatgtcggc
     4381 caggaggtct tcctacggct gtattattcg ctcgaccaat tgcggacggc ggaagttttt
     4441 gagccgtggc tgtaccgctt gacggtgaac gcggcgtatg actatctccg aaaacagcgc
     4501 cggcagcggg aagccaggat ggctgatttg acggaggagc aagtactggc ggcggacgcc
     4561 gcagtgggcg gcaagcaggc cttggaggag gatcagaaag cgcatatccg ggagttggtg
     4621 cagtcccttt tgggcaaggt ttcggaggaa gaccggatat tgctggtgct gaaggaagtt
     4681 cagggcctgt cgttgaagga attggagaga atttaccggg tgaaagagaa cgcgttgaag
     4741 gtcagattgt tccgagcccg gcagcgcgtg ctaaaggcgt ttgaaggaat tcagaagcag
     4801 gagctgaaaa tcaagactct tgccacaggc gggaaacatg ttggacgaaa agcggtctga
     4861 cggattggag aggttgtggg aggcctaccg gcttgcaact cctgagccgg ccgtttcggt
     4921 taacttcatg ccgcaactgt gggcgaggat cgaagcggcc agaccaacaa gctggaccat
     4981 gcccctgacg cggctggcct cgcgcctggt tccgctcgcg gcggcgctca ctctggcgat
     5041 gagcgcctat ctgtggattc cgcagtccag gacaaatgcg aatagccaga ccggttacgt
     5101 ggacgtgctg gcatccgact tgctcgagca acagcgcccg gcgctgtggg tagccaatgc
     5161 ggaggacagc atttagatga caaaacccac tcgcatcgcc gctctgtgcc tgggaattgt
     5221 gtttctggcc ggggccattt ttggctttgt cgcccacggc ctttacgccg agcacacgac
     5281 tcgagcctcg aatccgcagc agatgcgcga gcgctacatt tccaatttgc aaaaggagct
     5341 ggcgctcagt cccggacagg ttgcgcagat cacatcgatt ttagatgaga cccgccagcg
     5401 tttccgcgaa attcgcgagc gtatggagcc ggagtttgag tcgctgcgcg aattgcagcg
     5461 cgagcggatc atgggcattt tgaccccgga gcagcagccc aagtaccagg ccatcttaga
     5521 gcagcatcgc cgcgaacagg aacgccgccg gcagcaaaac acgaaagccg ggcattgaca
     5581 ggtgagcccg cgactcccca accgttagcc cctcgccgtt tttccgctcc cgggcatcgc
     5641 cccgagcttt ccgccatccc gccctcatag gtcttcccgc caccaaaaaa gcgtgcgagc
     5701 cggtccaaag aagcgcttcc tggcagcatt gcggagttgg ttgatcaggt catccaggcc
     5761 agggattccg ctctgatcga cgtgcgccga ggccgccggc gtccgtaatt agcggcaagt
     5821 cttatggggc ttctgcgtac acgcggcgga cgacgtttgc gacgctgttg atatttgtgt
     5881 attccggata cttagccttc agctcgttgg tcactgccgc cgctgcctct tctatgcttt
     5941 tcccctggcg cttcagttcc agggcgcggc tctgcaaatc cagcatgaac gtccgctgct
     6001 tggcgatgag agagccgtcg ccaagctggc cgtgatcggg cacgatgaag cgcggctgga
     6061 gcggctcgat ttggtcgagg atggcaagcc agcttttggg gctcgaatcc gcattgggca
     6121 ttccggggac gagattcttt tgcacgatgt ctccggagag cagcgtgcgg tcctcctcca
     6181 cgaaaatcaa ctcgtcgccg cgggtatgcg ccgcaccgag ccagaacagg cgggcggtcg
     6241 ctcccccgag atcgagtttc acctcgcggt cgaaaatcac gtccggcctg cgcagtttca
     6301 cgtcttgcag caactcccga ttcagcgccg agcggccccg aaacatctcg atgaactccg
     6361 atccccgctg ctccatttct tcctgctgca cggcggggcg aatcaggatg gtttccagcg
     6421 gaaatcccgg ctccccggcg gcgtgctcgg ggtggaaatg agtcgtcgta agatatagca
     6481 gcggatgttt ccccagcttc gccgcctcgc gcgctactag cgcgccgttg cggcggccca
     6541 tccccgtgtc gacgaccagg gtagcgcggc tgccgactac aatcccgata tttggaaatc
     6601 ccaaaatggc ataaacgtgt tccgacactc gagtgacgga gttttccggg agtagaggcg
     6661 attgcggcgc cgggttctgc gcgagagccg cagcttggaa aatcgctgcg gcgcacaaaa
     6721 aggcggcgaa ggcccgaaag ggaacccgcg tcggcggcat aggcttttcc ccctgttggc
     6781 tgcgcgaatg gaagagcgtg gcctattcta caacagttga cgaaccgccg ccgcggctgc
     6841 tggcagcggc tccgccgaat attcacgttc catgccaggc gcccgcgatg agaaccgccg
     6901 gccggagttg acgccagagc cgccccgcgg aactcatttc tacgggctgg ctttcgtgat
     6961 aacttggggc cgtcggttgt ggacagcgga gagcgggccg ccagttctgg gcggcagcgg
     7021 ctggcgccga aggggctcgc cggaagccac aagaccggga gcccggaccg ccgggcggcg
     7081 gaggatgcct atgagatcga aggcaaagac gttggtcctg tgggtggcag ccgtggctgc
     7141 catcgccttc tttgtgccgg cctcgccggc cgatcctttg tcttgcgcgt tcggggaata
     7201 taaaccaatt ccgggtctgg cagcgcagat ggccggcgac acgctgacct tgaattggga
     7261 tggcgaagga gatcagcaat tgcggctgcg cctggggatc aactccggca ggccaactat
     7321 gcgggaactg gccgtaaggc acagaggttc cccatggatt gcggcggcta ccaacgcgac
     7381 gcccgaattt cgggtcgtct ccggcctccg gcgcataacg aatcagcagc tcgatccttt
     7441 ggctggcctg ggcgtgccca tcacgcaaga aatcatcgac aagtacaagt gggaggcgtt
     7501 ctgggacgcg ccattaaatg tgccgggagg cgagggcgcc cacaacaata ccactccgcc
     7561 acagcgaggg gttctcaacc agccggggct gccccgcagc ccggatgaaa tcaagcgcgc
     7621 ctccgctgtc taccatgtga cggcttgcga ggtgagaacc aatggcgctc gtcttgaaat
     7681 ttcctttccg ggcgttcaac ttggcgtgtt tgcagggcgg ttgcaattta cggtttacaa
     7741 gggcgccaac ctgatccggc aggaaatcat cgccaagacg gaagagccct cggtggctta
     7801 caaatatgac gcggggatac gcggcctggc gatccagccg gggtcccgcc tggtttggaa
     7861 agggcttacc aacttatggc aggaataccg gctgggcggt ccgcccaatg agggtccggt
     7921 ggttgtcaaa tccagcagcc gcctgctagc tgccgaaaca gcgggggccg ctctcgccgc
     7981 ctttccgccg ccccacacct ttttctgggc ccgcgagacc tcctacaacc ttgggtataa
     8041 ctggtatcaa aaggagagcg gctcctcatt ttctttcggc attcgccagg ccgagcggga
     8101 ggagagcgca gccgatgaga atcggggcag cgaggaccgg agccagaact tcgcgctact
     8161 cagcgcgcgg ccgggcacgt ggcagaggat gcccgtttat ttctacgtga gcgccgactc
     8221 cggccctgcc gcagtcgagg gagcgctcct ctacacgcgc ggagaccgct tcaaggccct
     8281 gcccggatac caggtgatgg cgacgcactt tcacatgggc ctggtggcgc gcgcccgccg
     8341 cgccggtggt ctggatgcgc tgctgcccga cctcgagatg atgcaggcag ccggaatcaa
     8401 cattgttgct ccgattgacg gtggcgcggg cttcgccccg gaaggcatcg ggcagaccgg
     8461 acggttgcgc atggcgccca tggatgatcc caagtggttc caatggagcc gcggtttggg
     8521 cgctcctgcg ggcgccgagg aggcgaccgg agccgcggac ggcgcttccg ggtctcctcc
     8581 cgcccgcgcc tctgcccgca gctccacggc ccgccgaatc tccggtgatt tgggaagcat
     8641 tgatccctac aagagccagg ccgattacta cgaggcggca aggcggcact ccgaccggag
     8701 cttcgtggtg atgccgaata ccgaattgat gcgcggcgag gtcgcccgca cgctgggagg
     8761 ccattccgat attctcattt cgcatcccgt ctactggacg caggggcggg ctcccggcca
     8821 gccgctttcc gaggatcatc cgaaatacgg gagagtctat catctgggcg acacggcgga
     8881 catgatggaa atgattcgcc gcgaaaacat gctcatcttc atgccccatc cccgctcgaa
     8941 gggatcgaca ggctttcccg acgcgatcaa agacacgccg cacttcaccg acgaaaacta
     9001 tcggggcatc ggcttccgct ggggaatggg cttggacggc tccgagcggc gattgtgcga
     9061 atatcgctgc
//
LOCUS       DASREK010000136        24516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_896285, whole genome shotgun sequence.
ACCESSION   DASREK010000136 DASREK010000000
VERSION     DASREK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 24516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24516
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896285"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFA54_06765"
     CDS             <1..407
                     /locus_tag="VFA54_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50541.1"
                     /translation="YVCDRANDRLQVFKRDGTFVKEAFIAKRTLGDGSVWDIAFSKDP
                     QQKYLYLADGANEKVYIIQRDTLEVLTSFGDGGRQPGQFYAVHSIATDSKGNIYTTET
                     YHGQRVQRFLYKGMGPVTRKDQGAVWPKTARN"
     gene            complement(479..2929)
                     /locus_tag="VFA54_06770"
     CDS             complement(479..2929)
                     /locus_tag="VFA54_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZS50542.1"
                     /translation="MALTSGARLGPYQILSPIGEGGMGEVYRAWDLRLEREVAIKVLP
                     SHLAGEPEMLARFEREAKAVAALSHPNILAIHDFGREKDFCFAVMELLEGETLRSRLE
                     RSPLPWREALEIGTGVAEGLAASHSKGIIHRDLKPDNIFLTFDGRVKILDFGLARVAT
                     PFSSSQETLATLRTPRRPATTEPGTVMGTFGYMSPEQARGEQVGVCSDIFALGCVLYE
                     MVTGRQAFARPTAAESIAAVLKEDPPEFKSFAGGGVGGAAPQGAAPGEGPETVPPEFQ
                     RVIFRCLQKKPADRFQSARELAAALKAIERASVSSQPQASPAPAEPAKTGARKVRMLG
                     MAAAVAAVVAGALAFFSLERRQSIDSLAVLPFINASHDPNAEYLSDGITEGIINNLSQ
                     LPNLGVMSRSSVFRYKGKEADPEAVGRDLHVRAVLVGRIVRIGENLSVSAELVDTRTN
                     QQIWGEQYNRKFSDLMAVQGEITREISDRLRLRLSGEDKARIARRPTENAEAYQLYLQ
                     GRYQWNKRTLDGMQQSIDLFQQAISKDEHYALAYAGLADSYALLADYNVLPAREVMPR
                     AKTAAMKALELDDRLAEAHASLGWAKLSHDWVWQDAEKEFNRSIELNPNYATAHQWYG
                     EYLTIMGRFDEALKEMNRSVELDPFSLVANTAAGSALYYAGRYDQAIEQLRKCIAMDQ
                     NFGPAHLFLGRAFKQKAAYAEASAEVQRALDLSGGGSNELAALGHTFAIAGRTLDAVK
                     ILDDLKERSKQTYVQPVGIAGIYIGLGDKDRAFEWLQRAYEDRSGWLIYLNVDPMFHP
                     LRQDPRFRELIRRVGLPY"
     gene            3088..4386
                     /locus_tag="VFA54_06775"
     CDS             3088..4386
                     /locus_tag="VFA54_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50543.1"
                     /translation="MMRHIWGLGCLIFGVASVAWAADSPATAEQVQALLARIEQLEKR
                     VAQLEAGENNGGAPAPSAAGQTTAPVPKAPPAPVLEPQTTMTHEQQMRMGESTQAPFA
                     SPLLRLSGFGDIDFSATDQRGVKSGFNEGQFVLHTSSTLSPHVSVFSELSLTARADAG
                     LGSPPATGFNAEVERLFIRFDQSDAFKASFGRYHTPINYWNTAFHHGAWLQTTISRPE
                     MVQFGGSFIPVHFVGGLLEGEAPTGGLHLNYDVGIGNGRGAVLSRAGDAGDINNNRAW
                     LVNIFAKPDKLFGLEVGGSAYRDKITVGGAAFDEWITAGHIVWHKENPELIAEIANVN
                     HSQVGRPGDSNSLAYYVQFAYRLPGFAQLWKPYYRWEYIHIPQSDILFVPRVSGLAGS
                     TVGVRYDITSFAAFKVEYRNQRRPGLPRINGAFAQTSFTF"
     gene            4400..4903
                     /locus_tag="VFA54_06780"
     CDS             4400..4903
                     /locus_tag="VFA54_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50544.1"
                     /translation="MTRRSAFPRLCAAALAARGWLSTARPLPMLAAGPAGDVAVVVNP
                     GVPATDLGFAEIRKLLLGDRQFWNSGLRVTLLMRAPVARERDVVLREIYQMSEAQFRQ
                     YWIAKVFRAEATSGPKVVYSDEEAAQMVAAIPGAIAFLDASKVPKGLKVLKIDGRLPG
                     EAGYKLR"
     gene            complement(5071..8397)
                     /locus_tag="VFA54_06785"
     CDS             complement(5071..8397)
                     /locus_tag="VFA54_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS50545.1"
                     /translation="MGSRNWLFALWAVLGLACHVYGQAQSGTVVGIVTDQAGAVVPNA
                     TVTILNEGTRLTRVVRTNENGQYVAYSFPTGQITVTAECPGFQKLVRSGIQLTAADTL
                     TVDLRLTVGNVQETVEVTGEAPLLQSQTAAVSTLINNQQMLEMPLNGRSLTQLLQLGT
                     GAAPQNPGLPTGLSGYQMRANNSVSVNGSVWNNNSFLIDGMFDRGLWLNGLTIVPTID
                     SIQEMRVMAANYSAEYGAAAGAVTVVQTKSGTNQFHGDAYEFLRNDHLDANNFFSNRA
                     GLVKPHFRRNEFGGVLGGPIRRDKTFIFGDYQGIRLAQPTTTVSTIPSLAQRQMVSTG
                     DFSSLSAPIYDPYSSPAGSQPRAQFPGNRIPIQMIDPVAIKLMNLLPAPTSSSATRNL
                     TFNPATTERVDQFDVRADQNLGSSDRLFFKYSYDNSSSLGTGSIPAPPNPSVPLSAYL
                     TGGSTNTLKNWSASANYTKVFSAAIVNEARAGAVRWNSAFVTANDPYNIATALGIPGL
                     NISDTSRGIPGYQMSGFTTIGDTAQSPEFARTISYQIADTLTWVKGSHAVKFGGSYIR
                     HDFNGHTAIAPRGWYTFNGQFTRQIGGSGGSVLADFALGAFATVTRSIQNGFFGMRYW
                     EGSLFAEDAWRATNRLTITYGMRYELQAPPHEVNNRWTNFDVATGRFLQNGANGLGSA
                     LRTLDTNNLAPRLGIAYMLTSDRKTVLRTGAGISYVEAYNAGKQLHQDPPLTISQLLT
                     ADINAAPPFTIAAGLPLPPTPDLSHPEIYSGNVIAFDQHLRDAKAMQWSFGIQRELIS
                     NLMLDVAYVGSRTLDMINSMNANQALPGPGALNPRRPLYSLDPLLGDVDYRTNWGASK
                     YHSLQVNLVKRYAKGLTASLAWTWSHNLANARGPSTSVQPQNDRCSACEWSNALEDRR
                     HMVVINHVYELPFGAGRQFANQGLLAHIVGNWNISGIWSMYTGASFGPSLATSVSNGQ
                     SSNPAVSAIERPNRVGDGNLPAGQRTIDHWFDTSAFAIPAQFTFGNSGLLILEGPGYF
                     NVDAGIHRDFPVREGMKLTFRWEMFNAFNRANFTNPDQNAPGAAPNSGNGAQIGTATA
                     GTISSAYPARSMQLSLKLVF"
     gene            8669..11650
                     /locus_tag="VFA54_06790"
     CDS             8669..11650
                     /locus_tag="VFA54_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZS50546.1"
                     /translation="MVPAKVGLCLAAVAAVASCQDVSEKTEFFETRIRPVLANNCYAC
                     HTDNKLGGLRVDSRVALLAGGKSGPAIVPGRPDESLLIRAVMQVDPKLKMPFGGNKLK
                     DKEIADLRYWIQAMSAFWPNEDAKPKPAAAQTAAFTIRPEQRNFWSFQPIRKPAIPPV
                     KNSGWVKTPIDNFILAKLEEKALKPAGPADKRVLIRRAFYDLTGLPPTPEQVDAFLSD
                     NSPEAYTKIVDRLLASPHYGERWGRHWLDVARYADGSGIPDRRPVFLGYGMARDGYAN
                     TWRYRDWVIQALNDDMPYDRFVKAQIAADLMPEKERAKLLPALGFFGIGPWFTGDDVV
                     FVEARANERDDKIDALTKGFLGLTVSCARCHNHKYDPISQKDYTALAGIFASSGYWEY
                     NLAPPERVDAYQRQRKKVKAAETALQEYADACALRVAETLASQIPEYMMAVRKVMLTD
                     PKADLKKVAEAEKLDPETFQRWFNYLTKAEKLHPYLKGWDALMAKGGGSEAEMRKIAE
                     EFRDLVLKVIPEKKAVIAANQEMVRDYKPDPNEATVLLPGDLIQFELFQFKQQMVQKV
                     MDTNHFYVWLDVVQGEDDQSYVRKDGIFEYRGKDLLRFLTPEEKAKLDAMQAEITALT
                     KAMPPEYPYLMGLKEEAKPVNVKLAVRGNPHNLGEEVPRGFPAILANTNGDPLPFTQG
                     SGRLQLAEAIVKHPLAARVMVNRIWQHHFGRGIVDTPSNFGMTGERPTHPELLDYLAA
                     RFIESGWSMKAMHREIMLSAAYQLAYEHIEANEAKDPDNRLVWRANFRRLEIEPLRDS
                     LLFVTGTLDERVGGPPQNLNNPNFKKRTVYARAARTPDGLLTLFDYPDPNITSEQRSV
                     TNVPLQGLFFMNSDLVARQAEALVARLGPEGATEEERIGRIQRAYRILFAREATQAEV
                     QRGLEFLRKAEILYNRPDAGAPKGQASAAGAAASAGRRNRRAAADGEPAEPPPAPPIN
                     FTPWQQYAQALLSAGEFYYIN"
     gene            11666..13069
                     /locus_tag="VFA54_06795"
     CDS             11666..13069
                     /locus_tag="VFA54_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZS50547.1"
                     /translation="MRHRSILPPLTRREALRQFAAGLGMAGFAAAAARPLPAAPVQGN
                     PWTPKAPHFAPKAKHVIVIFLQGGLSQVDSFDYKPMLDKYDGKPLPYETPRTEFATGN
                     LMRSPFKFKKYGQNGIEVSELFPHMAEIIDEFCIVRSMISDIPNHGPSVLMMNTGSVR
                     VGRPSMGSWVVYGLGTENQNLPGYIVLTAGGGGGGDSFGSAFLPAVYQGTLVPYTQSD
                     PNKQIRYLANAHMNLAQQREQLDLLRDLNQMYVDQLQGAPELEATIQSMEVAFRMQTE
                     APEVFDTRKESPATLAKYGEDGIGRACVTARRLVEKGVRFVQIVHAGWDHHADIMGHK
                     HTARAVDQPIAALVQDLKDRGLLHDTLVLVTSEFGRTPVLNLGGFRSVHNGRDHNIYG
                     FTILMAGGGARPGTIYGATDDFGFKVAQNPVHVHDLHATILYLLGMDHERLTYQYAGR
                     NFRLTDVEGRVVKDIVA"
     gene            complement(13111..13599)
                     /locus_tag="VFA54_06800"
     CDS             complement(13111..13599)
                     /locus_tag="VFA54_06800"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HZS50548.1"
                     /translation="MSFLAVCQWIETTSLSIAIREGALYYPILGAFHLLGIAWFGGML
                     LMSDLSVLGIGLRHEPVSEVWSQFRRWKWAGFAIIVLSGSLLWWAEPVVCYKSLSFRI
                     KMVLFSFAVLNTLMFRRTAYTGLAAAEKGPVKSRSARLSAYASLLLWLALIFSGRGIA
                     FF"
     gene            complement(13797..14285)
                     /locus_tag="VFA54_06805"
     CDS             complement(13797..14285)
                     /locus_tag="VFA54_06805"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HZS50549.1"
                     /translation="MSVETLLLHFFRWLGNTPVANSINDSEWLFPIIESLHITGLAVL
                     IGTVAIVDFRLLGLVLRNRPAPEVAGDLKGWTLAGLGLMLTTGPLMLSAEPERGYGNP
                     AFEFKMSCLLLALLTHFTIHRRVTRRDQPSETTVAGKIAGALSLALWTGVVLGGRAIA
                     FF"
     gene            14499..15320
                     /locus_tag="VFA54_06810"
     CDS             14499..15320
                     /locus_tag="VFA54_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50550.1"
                     /translation="MRKRFQGLVIGIPAAAAALLLAAISAFGQSSAVYRAPRTSDGKP
                     DLNGIWQALNTANWDLQGHAARPGPVVALGAIGATPAGLGVVEGDEIPYLPSALAKKR
                     ENFEHRLTADPEVKCYLPGVPRATYMPFPFQIVQTPKYILIAYEYASASRIIYMENAP
                     PSPVDTWMGNSTGHWEGDTLVVDVTSFNDQTWFDRAGNFHSDALHVIERYTPLTHDAL
                     SYEATIEDPKVFSRPWKIRMPLYRRLESNAQLLEFKCVEFVEELMYGPLRKQPSK"
     gene            15334..16377
                     /locus_tag="VFA54_06815"
     CDS             15334..16377
                     /locus_tag="VFA54_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50551.1"
                     /translation="MGYRSLRAISALALVLGGLGIPPPAACQTPAAPGTTQVAANRAP
                     AAATWTLPRTPDGQPDLQGLWTNATLTPFERPKEFAGKEFFTEQEAVEFARRVLDNAN
                     RDRRGTTPEADVAGSYNESWFDRGTAVAPTRRTSIVIDPPDGRVPPLTPEARKTMAAL
                     AAIRRRPPEGPEDLGLQDRCIIWPTAGPPMVPAGYNNNYLILQTPQYVAILIEMIHDV
                     RIIPLDGRPHLPQSIRQWMGDSRGHWEGDTLVVDTTNFTDKTHFRGSDQNLHLTERFR
                     RTGPDTIEYRFTVDDPTAFTRPWTGEISMLKTPGPIYEYACHEGNYAMTNVLSGARAQ
                     EKAAAEAAKKGSK"
     gene            16523..16939
                     /locus_tag="VFA54_06820"
     CDS             16523..16939
                     /locus_tag="VFA54_06820"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HZS50552.1"
                     /translation="MRTKLAIVVAAAGLLMTAGPAWSHHAFAAEFDAKRPVKLRGKIT
                     KMEWINPHAWIHMEVIGPDGKPVNWMVEAGTPNTLFRRGFTKDSLKPGTEILVDGYQA
                     KDGSNRANGRDVTFPDGRKLFLGSSGTGAPYDNERK"
     gene            complement(17124..17600)
                     /locus_tag="VFA54_06825"
     CDS             complement(17124..17600)
                     /locus_tag="VFA54_06825"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HZS50553.1"
                     /translation="MSLLPFFQWCENSAVGEAIRSSTWLFPVIESFHLLALAVIGGAV
                     LVVDLRLLGFGLRRQPVAQLAIDAQRWLIGSLMVMIASGILLFTSEAIKCYYHEAFWF
                     KMTCLALAILFTFTVRRKVTLADGAPVAPIRSKLVGLISLALWCGVGIGGRWIGFS"
     gene            complement(17609..18211)
                     /locus_tag="VFA54_06830"
     CDS             complement(17609..18211)
                     /locus_tag="VFA54_06830"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HZS50554.1"
                     /translation="MSVLRFCEWLANTQWSIALHESTWGYPIVESVHVLTLCLFLGTA
                     VMLDLRLLGLTMRRAPVSEVVDRLLPWTTAGFAVMVASGILLFYAIPVRTFQNIFFRI
                     KFAMLVLSGLNAWVFHAGVYRKVKEWDLDPVPPTRARIAAGLSLLLWAGIVICGRMIA
                     YNWFDSGPIAATTSEAASSKARNPQPRPLFPPSPQGRAAE"
     gene            complement(18382..20040)
                     /locus_tag="VFA54_06835"
     CDS             complement(18382..20040)
                     /locus_tag="VFA54_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZS50555.1"
                     /translation="MTKFKPSTQVDFVVIGAGGAGGVVAKELSSAGFQVVVLEQGPYL
                     REKDFSHDELKIRSTPALVNDHALQPNTFRRSEKEKAVLAPAVSYGRCVGGGTVHYTA
                     SQSRFHEVDFIERTRWGPIGGTGLADWPITYAELEPYYTKVEWELGVSGLGGGSPFES
                     PRSKPYPVPPLPIKSSGVLMERAAKKLGLHASPVALAILSQPYRGRKACNNCGFCEWF
                     GCEIGAKSSTLSTVIPIAEKTGRCEIRPNSYVREISVDKSGRVTGAVYFDAEKKENFQ
                     RAKAVVLCANGTETPRLLLLSKSSLFPDGLANSSGLVGKFLNFGAGASAHGTFEQPLN
                     EYKSVQSSRMIEDFYDSDPKRGFYGGGRLDARWGYMGPIGYALQGLPPDAPRWGAGFK
                     QACKEYFSRTMTIQAFCTSLPLETNTITLDPDVKDAWGLPAMRVTYREHPDTLKTKRF
                     FVDRALEILDAAGAIKKWASPVTESRSGGHLMGTCRMGNDPKTSVVDKFHRAHDVPNL
                     FIVDGSSFVTIGRNHPTCTIEALAYRCSEYIIQAAKRGDLKAPA"
     gene            complement(20070..20642)
                     /locus_tag="VFA54_06840"
     CDS             complement(20070..20642)
                     /locus_tag="VFA54_06840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZS50556.1"
                     /translation="MKKRGASRRDFLAGSVSAWLAASWPGIVAAQEHVHRLARSGAAG
                     KFEFFSPEQAAEVEAVAAQIIPSDDSPGAREARIIHFIDRALHTFDRDKQPVYTEGLR
                     DLQAKTATMFPGVAKFSALTSAQQIELLKSIEKTPFFGMIRTHTIVGFFANPEYGGNY
                     EKTGWRLIGFDDAFHFQPPFGYYDRDYKGA"
     gene            21169..22665
                     /locus_tag="VFA54_06845"
     CDS             21169..22665
                     /locus_tag="VFA54_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS50557.1"
                     /translation="MRKIILFLPPYSGPVFGPPLALLALAAPLREAGYEPKIIDGVIT
                     PDYPGVLRAEIANALCFGVSLLTGPMISHAARAARLVKQLRPDIPVVFGGWHPSLLPE
                     QTLREDFVDVVVRHQGEITFLEVVRRLESRQPLDLVSGCSFKRGGKICQNPDRPVAAL
                     SSLPAPLFEAADFDAYEKVSGERAAPYVTSVGCPYACDYCTDTVFYKRRFNALPAERV
                     VNEMTGLVRRHRLTKIALCDSNFLVDARRALEIARGIMASGLRFRWSFQASTDLLCRM
                     SDDEVRLLGESGVEHIGFGTESASEDVLRQMNKLHQRVPDMFETARKCRQAGIRATFN
                     LILAYPGETEADREVTLRVMSDIGRRYGNVTFSPNIFTPYPGIPIWPELRRMGVSEPR
                     SLEAWSSISLGRNVLPWLQGRPYRSLKRNVSFFLLTNQISRAAREPGSSVRRLILRIV
                     SVLLNWRVRFGFFHFPLDLWLAHATKRLAARRSLLTGRPLAGSLNEAC"
     gene            complement(22826..24214)
                     /locus_tag="VFA54_06850"
     CDS             complement(22826..24214)
                     /locus_tag="VFA54_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZS50558.1"
                     /translation="MGELIYASATAQAREIRTGEVSSRELVQMHLKRIEAVNPKLNAV
                     VQLAAEEALEAARRADEDFAKGMIRGPLHGVPITVKDSMEAAGMTCTSGTLGRSKFVS
                     TADATAVIRLRGAGAIPLGKTNLPDLLFAFESDNLIYGRTNNPYDLSRTAGGSSGGEA
                     AAIAAGESPLGVGSDAAGSIRLPSHFCGIAGIKPTSGRLARTGHFPPPGGLFDALWQI
                     GPMARFVEDLILAMPILSGADWVDPAVVPMPWRDPNAVDLRGLRAAFYTDNGILPATP
                     ETVAAVEKAAASLAHSGVRIEEARPAGIEQTYELALGLFGADAGACLRELLQACGSQQ
                     AHPLLERFLSILGQRPLAAPEFGRLLIRVDRFRSAMLSFLEKHDLLLCPVCADAAMPH
                     GTTFDHLEAFTYTMSFNLTGWPAVVVRCGTSACGLPLGVQIAGRPWREDVALAVARKL
                     EADFGGWQRPPL"
     gene            complement(24202..>24516)
                     /locus_tag="VFA54_06855"
     CDS             complement(24202..>24516)
                     /locus_tag="VFA54_06855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS50559.1"
                     /translation="TSGIGIKMMGIGKPVLVTASEETSRFPELSVIRIDPDESEVEML
                     GHYLRALEQNPEMRAEIGRRAAAHIAENHSLKRAVQVYLDLIEATARRPVELRERTAW
                     GN"
BASE COUNT         4883 a         7370 c         7530 g         4733 t
ORIGIN      
        1 tctatgtctg cgaccgcgcc aacgaccggc tccaggtatt caagcgggac ggtacgttcg
       61 tgaaagaagc cttcatcgcg aaacgaactt tgggcgacgg ctctgtgtgg gacatcgctt
      121 tctcgaaaga tccgcagcaa aagtatctct atctggctga tggcgccaac gagaaggttt
      181 acatcatcca gcgcgacacg ctggaggtct tgaccagctt cggagacggc gggcgccagc
      241 ccggccagtt ctatgccgtg cacagcatcg ccaccgactc caagggcaac atatatacga
      301 ctgagactta tcacggccag cgcgtgcaaa ggttcttgta caaaggcatg gggcccgtta
      361 ccagaaagga tcaaggcgcc gtctggccga aaacggcgcg aaattagacg ctgggaaggc
      421 aacttgcaaa cggggaagcg gttagcgccc gcggtgaagt ctatcgatca gctcgatttc
      481 agtagggcaa gccaacgcgc cggattaatt cgcggaagcg cgggtcctgg cgcaacgggt
      541 ggaacatcgg atctacgttc aagtaaatga gccagccgga gcggtcctca taggccctct
      601 gaagccactc gaaggcccgg tctttgtccc ccagcccgat ataaattccc gcgatcccga
      661 cgggctgcac gtaggtctgt ttcgaccgtt ccttgaggtc gtccaaaatc ttaaccgcgt
      721 ccagcgtgcg gccggcgata gcgaaagtgt gtcctaacgc ggccaactca ttgctgccgc
      781 ccccggaaag atcgagggct ctctggactt cggcgctcgc ctccgcatac gcagccttct
      841 gcttgaaagc ccgccccaga aacagatggg cgggaccgaa gttttgatcc attgcaatgc
      901 atttgcgaag ttgttcaata gcctggtcat agcggccggc gtagtaaagc gccgacccgg
      961 cggcggtgtt cgccaccagg gagaacgggt ccaactcgac agagcggttc atctccttca
     1021 atgcttcgtc gaaccgcccc atgattgtca ggtactcgcc gtaccactgg tgggccgtcg
     1081 cgtagttcgg gttgagctcg atcgatcggt tgaattcttt ttccgcatct tgccagaccc
     1141 aatcatggct caatttggcc cagccgagcg aggcgtgggc ttcggccagt ctgtcatcga
     1201 gctcgagcgc tttcatggcc gccgtcttgg ctcgcggcat cacctcgcgc gcgggcagga
     1261 cgttgtagtc agccagcagc gcataggagt cggccagtcc cgcgtacgcc agggcataat
     1321 gctcgtcctt gctgatggcc tgctgaaaca agtcgatgct ttgctgcatg ccgtccagcg
     1381 ttcgcttatt ccactgatat cgcccctgga gataaagctg gtaggcttcg gcgttctccg
     1441 tgggccgcct ggcgatgcgc gccttgtcct cgccggaaag ccgcaggcgc agccggtcgg
     1501 aaatttccct cgttatctcg ccttgcacgg ccatgagatc agagaacttc cggttgtact
     1561 gctcgcccca gatctgctgg ttggtgcgcg tatccaccag ttccgcgctg accgaaaggt
     1621 tttctccgat gcgcacaatg cgtccgacca gcaccgcccg cacgtgcaaa tcgcgcccca
     1681 ctgcttccgg atcggcctcc ttgcctttgt accggaacac ggagctgcgc gacatcaccc
     1741 ctaggttggg aagctgtgac aagttattaa taataccttc tgtgattccg tcgctcaaat
     1801 attcggcatt aggatcgtgg ctggcgttga tgaagggaag gacagccaga ctgtcgatgg
     1861 actgccgacg ttccagcgaa aaaaaggcga gcgcgcctgc caccactgcg gcaaccgcgg
     1921 ccgccatccc gagcattcga accttgcggg cgccggtttt tgccggctcc gccggagccg
     1981 gtgaggcctg cggctgcgag gagacagagg ctctctcgat ggccttcagc gcagcagcaa
     2041 gctctcgggc cgactggaag cggtccgccg gctttttttg aagacagcgg aaaatgaccc
     2101 gctggaattc cggcggaacc gtttccggcc cctccccggg ggcagcgccc tgcggcgccg
     2161 cgccccctac gccgccgccc gcaaagctct tgaactccgg cggatcttct tttaaaaccg
     2221 cagcgatgga ctccgcagcc gtcgggcgag cgaacgcctg ccgcccggta accatctcgt
     2281 agagcacaca cccgagcgcg aaaatgtcgc tgcacacgcc cacctgttcc ccgcgggcct
     2341 gctcggggga catgtagcca aatgtgccca tcaccgtgcc gggctcggtc gtggcgggac
     2401 ggcggggcgt cctcagcgtc gccagcgttt cctggctaga agaaaaaggt gtggccacgc
     2461 gcgccaggcc aaaatcgagg atcttcacgc ggccgtcaaa cgtcagaaaa atgttgtccg
     2521 gcttgagatc gcgatggatg atgcctttcg agtgcgaggc tgccaggccc tcggcgacgc
     2581 ccgttccgat ctccagtgcc tcgcgccagg gcagaggcga gcgctcgagc cggctgcgga
     2641 gcgtctcgcc ttccagcagc tccatgacgg cgaagcaaaa gtctttttcc cggccaaagt
     2701 cgtgaatcgc cagaatattc gggtgcgaga gcgccgccac agccttggcc tcgcgctcga
     2761 agcgggcgag catttccggc tcgccggcaa ggtgcgacgg cagaactttt atcgccacct
     2821 cgcgttccag ccgcagatcc caggcccggt aaacctcccc cataccaccc tcgccgattg
     2881 gggaaaggat ctggtatggg ccaagacgag ctccgctagt caatgccatg ggaaaaacca
     2941 ataagcgcca cctagtgtag cgtttttttc ggcctggcgt tggagactct ggtggcggcc
     3001 cgatcttttc ctttgtttct cacgggtctt ggtctacact acctgctttg agaaggcttc
     3061 ggccctatat atagcgggga gatcatcatg atgaggcaca tatggggatt gggctgctta
     3121 attttcgggg tcgcttccgt ggcttgggca gcggattctc cggccacggc cgaacaagtg
     3181 caagctctgc tggcccggat tgagcaactg gaaaagcgtg tcgctcagct tgaggccggc
     3241 gaaaacaacg gcggcgctcc ggccccgtct gccgctgggc aaacgacagc ccctgtgccc
     3301 aaagcccctc ccgcaccggt tttagagccg caaaccacca tgacgcacga gcagcagatg
     3361 cggatgggtg aaagcaccca ggctcccttc gcttctccgc tgctgaggct gtccggattc
     3421 ggtgatatcg atttttcagc gacagaccag cgcggcgtga agagcggatt caatgagggg
     3481 cagtttgtcc tgcacaccag ctctacgctc tcgccccacg tcagcgtctt ttccgagctc
     3541 agcctgacag cccgtgccga cgcgggcctc ggctcgccgc cggcgacagg gttcaacgcc
     3601 gaggtggagc gtctttttat tcgcttcgat cagagcgacg ctttcaaggc gtcttttggg
     3661 cgctatcaca cgccgatcaa ctactggaac acggccttcc atcacggcgc atggctgcaa
     3721 acgaccatca gccgccccga gatggtgcaa ttcggcggca gcttcatccc ggtccacttt
     3781 gtgggcgggt tgctggaagg cgaggcgccg accggcggtt tgcacttgaa ctatgatgtg
     3841 ggcattggca atggccgcgg ggccgtgctc agccgggccg gtgatgccgg cgatatcaac
     3901 aataaccggg cctggctggt caacatcttc gcgaaaccag acaagctttt cggcctggaa
     3961 gtcggtggat ccgcctaccg ggacaagata actgtgggag gcgctgcttt cgacgaatgg
     4021 atcacggcgg gacacatcgt ctggcacaag gagaatcccg agctgatcgc cgagatcgcc
     4081 aatgtcaacc atagccaggt gggccgcccc ggcgattcca acagcctcgc ttactacgtt
     4141 caattcgcct atcgtttgcc gggattcgcc cagctttgga aaccctatta ccggtgggaa
     4201 tatattcaca ttccgcaaag cgacattctt ttcgtcccgc gggtgtcggg cctcgccgga
     4261 tcgacggtcg gagtgcgcta cgatataacc agcttcgccg ccttcaaggt ggaataccgc
     4321 aaccagcggc gtcccggcct gccgcgcatc aacggcgctt tcgcgcagac cagcttcacg
     4381 ttttgagggg gcgacggcga tgacgcgacg atcagctttt cccaggcttt gcgcggcagc
     4441 gttggcggcc cgcggatggc tttcgacggc gcggccttta ccgatgctgg cggccggtcc
     4501 cgccggcgat gtcgccgtcg tggtcaaccc gggagttccc gccactgact tggggttcgc
     4561 cgaaatacgc aaacttctcc tcggcgaccg ccagttttgg aactccggtc tccgtgtcac
     4621 gctgctgatg cgcgctcccg tggcgcggga gcgcgacgta gtgttacgag agatttatca
     4681 aatgagcgag gcccagttcc gccagtattg gatcgccaaa gtattccggg cggaagcgac
     4741 cagcggcccc aaggtcgtct actcggatga agaggcggcg caaatggtgg ccgccattcc
     4801 cggggcgatc gcgtttcttg acgcatccaa ggttcccaag ggccttaagg tgctcaagat
     4861 tgacggccgg ctgccgggcg aggccgggta taagctgcgg tagcgctggc agggccccga
     4921 ccgctcaagc cgctggagaa tcgagccgca acagccatgc gcccgttatg cgacggctga
     4981 cgccccggct gccttctgcc gcgcagcgtg ctcaaccggc cggcagcgtt tcctactgca
     5041 ttttgcgcgc gtaaaatcag gccgagcggc tcaaaaaacc agcttcaagc tcaactgcat
     5101 gctgcgagcc gggtaagcgc tgctgatcgt gccggccgta gccgtgccga tttgcgctcc
     5161 attgccgctg ttgggagctg cgccaggagc attctggtcg ggattggtaa agtttgcgcg
     5221 gttgaacgcg ttgaacattt cccatcgaaa ggtcaacttc atgccttcgc gaaccggaaa
     5281 atcccggtgt atgcccgcat cgacgttaaa gtagccgggc ccttcgagga tgagcaggcc
     5341 cgagtttccg aaagtaaatt gcgcgggaat cgcaaacgcc gacgtgtcga accagtggtc
     5401 gatcgtgcgc tggccggcgg gaagattgcc atcgccgacg cggttcggcc gctcaatcgc
     5461 cgagaccgcg gggttgctgc tctggccgtt ggaaaccgac gtggcgaggc ttggcccgaa
     5521 cgaggcgccg gtatacatgc tccagatacc ggagatgttc cagttgccca cgatgtgagc
     5581 cagaagccct tgattggcaa attgtcttcc ggcgccgaaa ggcagttcgt aaacgtgatt
     5641 gatcacgacc atgtggcgcc ggtcttcgag ggcgttgctc cattcgcagg ccgagcaccg
     5701 gtcgttctgc ggctgcacgg aggtggaggg ccccctcgcg ttggcgagat tgtgcgacca
     5761 ggtccaggcg agcgaggccg tcaacccttt cgcatagcgc ttcaccaagt tgacttgcaa
     5821 cgagtgatat ttcgaggcgc cccagttagt ccggtagtcc acgtcgccga gcaacgggtc
     5881 caggctgtag agcggccggc gcggattgag cgctccgggt ccgggcagcg cctggttggc
     5941 gttcatagag ttgatcatat ccagcgtacg ggagcctacg taagcgacgt ccaacatcaa
     6001 gttggagatc agctcgcgct gaatgccgaa gctccactgc atggccttgg cgtcccgcaa
     6061 atgctggtcg aatgcgatca catttccgct gtagatctcg ggatgcgaaa gatccggtgt
     6121 tggcggcagg ggcaggccgg ccgcgatagt gaagggaggc gctgcattga tgtccgcggt
     6181 cagcaattgc gagatggtca gcggaggatc ctggtgcaat tgcttgccgg cgttgtaagc
     6241 ctcgacatac gatatgccgg ccccggtccg cagaacggtc ttgcggtcgc tggtcagcat
     6301 gtaggcgatg ccgagccggg gggcgaggtt gttggtatcg agggtgcgca gggcgctgcc
     6361 cagtccattc gcgccgttct gcaagaacct tccggtggcc acatcaaagt tcgtccagcg
     6421 gttgtttact tcatgcggcg gagcctggag ctcgtaccgc atgccgtagg tgatcgtcaa
     6481 gcggttcgtg gcccgccagg cgtcttcggc gaacagcgat ccttcccaat agcgcatgcc
     6541 gaagaacccg ttctggatgc tgcgcgtgac ggtggcgaag gctccgagcg cgaaatccgc
     6601 cagaacggag cctccggagc cgccgatctg ccgcgtaaac tggccgttga acgtatacca
     6661 gccccggggc gcgatagctg tgtgcccgtt gaaatcgtgt cgaatgtagc tgcctccaaa
     6721 cttgacggcg tgcgaaccct tcacccaggt gagtgtatca gcgatctggt aggagatggt
     6781 gcgtgcgaat tcgggcgatt gggcggtgtc tccgatggtt gtgaagccgc tcatctgata
     6841 gcccggaatc ccgcggctgg tatcgctgat attcaggccg ggaatgccca gggccgtggc
     6901 gatattgtac ggatcattgg cggtgacgaa ggccgagttc caccgcactg cgccggcccg
     6961 cgcttcgttg acgatggccg cgctgaaaac cttggtatag ttcgccgagg ccgaccagtt
     7021 cttcagggta ttcgtgctgc cgccggtcaa ataggcgctg aggggaacgc tcgggttcgg
     7081 gggagccggt atgctgcccg tgccgaggct gctcgaattg tcataactgt atttgaaaaa
     7141 cagccggtca ctcgagccca ggttctgatc ggcgcgcaca tcgaattggt ccacgcgttc
     7201 cgtcgtcgcg gggttgaaag tgagattgcg ggtcgcactg gacgaggtgg gggcgggcag
     7261 cagattcatc agcttgatgg cgaccgggtc gatcatctgg atcggaattc ggttgcccgg
     7321 aaactgcgcg cggggttgag agccggctgg agaactgtag ggatcataaa tcggcgcgct
     7381 gaggctgctg aaatctcccg tggacaccat ctgccgctgg gcgaggctgg ggatggtcga
     7441 aaccgtcgtg gtcggctgcg ccagccggat tccttggtaa tcgccgaaaa taaaagtttt
     7501 gtcccggcgg atgggcccgc ccaacacgcc gccgaactca ttccggcgga aatgcggctt
     7561 tacgaggccg gcccgattgc tgaagaaatt gttggcgtcc agatggtcgt tgcgcaggaa
     7621 ttcataggcg tcgccgtgga actggttggt gcctgatttc gtttgcacca cggtgaccgc
     7681 tccggcggct gcgccgtatt ccgccgaata gttggcagcc atcacgcgca tttcctgaat
     7741 agaatcgatc gtggggacga tcgtcaatcc gttcagccac aggccgcggt cgaacatccc
     7801 gtcgatcaag aagctgttgt tgttccacac cgatccattc acgctgaccg agttgttggc
     7861 ccgcatctga tagccgctca aaccggttgg caagccggga ttctgcggcg ccgcgccggt
     7921 ccccagttgc agcagttgtg tgagggatcg cccgtttagc ggcatctcga gcatttgctg
     7981 gttgttaatc agcgttgaaa cggccgccgt ttgagactgt aaaagcggcg cttcgccggt
     8041 gacctccacg gtctcctgaa cgtttcccac ggtgagccgc agatcaaccg tcagggtgtc
     8101 ggccgccgtt agctggatgc ccgaccgcac cagcttttga aagccggggc actcggctgt
     8161 gacggtgatt tgcccggtcg gaaacgaata ggcgacatac tggccatttt cgttggtcct
     8221 aacgacgcgg gtaagacggg ttccctcgtt gagaatcgtg acggttgcat tgggcacgac
     8281 agccccggcc tgatcggtta cgatgccgac caccgtgccg ctttgcgcct gcccataaac
     8341 atggcaggcc aggccgagga ctgcccacaa tgcgaaaagc caatttctgc tgcccatctg
     8401 ggtctccttt ccaaggttgt gcattggccc gcgttctctg caaaacaggg acgtggaaaa
     8461 agaccagttc attcagcagc cgccccggct ggtaaatcag aacaaaacga gacgccagca
     8521 tactataccc aaatcacaga aagagccaag caaaaattca gaatttacgc ggctatatcg
     8581 ctaaatgccg ctctaacttc cccttgatct ggacttcggg aggtggtaca aaggaccaag
     8641 ctcggctatt tcaccgccgg aggaccaaat ggtccctgca aaagttggtt tgtgcctcgc
     8701 agcggttgcg gctgttgcgt cgtgtcagga tgtttccgag aaaacagagt tcttcgagac
     8761 gcgcattcga ccggtgttgg ccaacaattg ctacgcctgc cacacagata acaagcttgg
     8821 cgggttgagg gtcgattcga gagtggcgct tctcgcgggg ggcaagtccg ggccggcaat
     8881 cgttcccgga agacctgacg aaagcttgtt gatccgggcc gtcatgcaag tcgacccgaa
     8941 gctgaaaatg ccgttcggcg gcaataagct caaggacaag gagatcgccg atctacgcta
     9001 ttggatccag gcgatgagcg cgttctggcc aaacgaagat gccaagccga aaccggcggc
     9061 cgcgcagacc gcagccttca cgatccgccc cgaacagcgg aacttctggt cgtttcagcc
     9121 gattcggaag cccgccattc cgccggtaaa aaattcgggc tgggtcaaga cgccaatcga
     9181 caatttcata ttggccaagc tggaagaaaa agcgctcaaa ccggcggggc cggcggacaa
     9241 gcgcgtgttg atacgccggg ccttctatga cctgaccggc ttgccgccca ccccggagca
     9301 ggtggatgct ttcctctccg acaactcgcc cgaagcttat acgaagatcg tagaccggtt
     9361 gctggcttcc ccgcattacg gagaacggtg gggacgccat tggctggacg tggctcgcta
     9421 tgcggatggc tccggaattc ctgaccggcg gccggttttt ctaggctatg gcatggcccg
     9481 cgatgggtac gcgaacacat ggcggtatcg cgattgggtg atccaggcgc tgaatgacga
     9541 catgccgtac gaccggtttg taaaagccca aatcgcagcg gacctgatgc ccgagaagga
     9601 gcgtgccaag cttctgcccg cccttggatt ttttggaatc gggccttggt ttacgggaga
     9661 tgacgtggtt tttgtcgagg cccgggccaa cgagcgggat gacaaaatcg atgccctgac
     9721 caagggattt ctgggactga cagtgagctg cgcccgctgc cataaccaca agtatgatcc
     9781 gatttctcag aaagactata cggctctggc cggtattttc gccagctcag ggtattggga
     9841 gtacaacctg gcgccgccag agcgggtaga cgcctaccag aggcagagga agaaggtcaa
     9901 ggctgcggag acggcgcttc aggaatacgc agacgcgtgc gccctgcgcg ttgcggaaac
     9961 cttggccagc cagattcctg agtacatgat ggccgtgcgg aaggtgatgc tcaccgaccc
    10021 caaggcggat ttgaaaaagg ttgcggaggc cgagaagctt gatccggaaa ctttccagcg
    10081 ctggttcaac tacctgacca aagccgagaa gctgcatccg tacttgaagg ggtgggacgc
    10141 tttgatggcc aaagggggcg gttcggaggc cgagatgcgc aagatcgccg aggagtttcg
    10201 cgatctggtt ctcaaggtga ttcccgaaaa gaaagcggtg atcgcggcca accaggagat
    10261 ggtgcgggac tacaagcccg atcccaacga agccacggtg ttgcttcccg gcgatctcat
    10321 ccaatttgaa ctgttccagt tcaagcagca gatggtccag aaggtaatgg atacgaatca
    10381 cttttacgtc tggctggatg ttgtgcaggg cgaggacgac cagtcgtatg tacgaaagga
    10441 tggcatcttt gagtaccggg gcaaggatct gctgcggttt ctgactccgg aggagaaagc
    10501 caagctcgat gcgatgcagg cggaaatcac cgcgctgacc aaggccatgc cgccggaata
    10561 tccctacctg atgggcttga aagaggaagc gaagcccgtg aatgtgaagc tggcggttcg
    10621 aggtaatcct cataatctgg gcgaagaggt gccgcgcgga tttccggcga ttctggcgaa
    10681 caccaacggc gatcccttgc cgtttacgca gggcagcggc cgcctgcaac tggcggaggc
    10741 gatcgtgaaa catccgctgg cggcgcgagt catggtgaac cgtatctggc agcatcactt
    10801 tggccgcggg attgtcgata cgccgagcaa tttcggcatg accggggaac ggccgacgca
    10861 ccccgagttg ctcgattacc tggctgcccg ctttatcgaa agcggctggt ccatgaaggc
    10921 catgcaccgg gaaatcatgc tgtcggccgc ctaccagttg gcctacgagc atatcgaagc
    10981 caatgaagcg aaggacccgg acaaccggct ggtgtggcgc gcgaatttcc ggcggctgga
    11041 gatcgagcct ctccgggatt ccttgctgtt tgttaccggc acattggatg agcgcgtagg
    11101 gggcccgccc cagaacctga acaaccccaa ttttaaaaag cggactgtct acgcccgcgc
    11161 tgcccgcacg ccggatggtt tgcttacgct gtttgattat ccggacccga atatcacttc
    11221 ggagcagaga agcgtcacca acgtccccct ccagggcttg tttttcatga acagcgacct
    11281 ggtcgcgcgc caagccgaag ctctggtggc ccgactgggt cccgagggcg cgacggagga
    11341 ggagcggatt ggaagaatcc agcgggccta ccggatattg tttgcgcggg aagccaccca
    11401 agccgaggtg caacgcggct tggagtttct caggaaagct gaaattctgt ataaccgtcc
    11461 tgacgccggc gccccgaagg ggcaagcatc cgcggccggc gccgcagcca gcgcggggcg
    11521 gcgcaaccgc cgcgctgccg ctgacggcga gccggcggag cctccgcccg ccccgccaat
    11581 caacttcacg ccctggcagc agtacgcgca agccttgctg agcgccggcg agttttacta
    11641 catcaactag gggtaaggaa ccgtgatgag acaccgatcg atattgccgc cgttgacccg
    11701 gcgggaggcg ctccggcaat tcgccgccgg cttgggtatg gccgggtttg ccgccgcagc
    11761 tgcccgcccg ctcccggctg ctcctgtgca ggggaacccc tggactccta aggctcctca
    11821 cttcgccccg aaagcgaagc acgtcattgt catctttctc caaggcggac tttcacaagt
    11881 cgattccttc gactacaagc cgatgcttga caagtatgac ggcaagcctt tgccttatga
    11941 gactccccgg actgagtttg cgacaggaaa cctgatgcgc tcgcccttca agttcaaaaa
    12001 atacgggcag aatggcattg aagtcagtga gctgttcccg cacatggcgg agatcatcga
    12061 tgagttctgc atcgtgcgct cgatgatttc cgacatcccc aatcacggcc cctcggtgct
    12121 catgatgaat acgggaagcg tacgcgtcgg gcggccgtcc atggggtcgt gggtagttta
    12181 cgggttgggc acggaaaacc agaatttgcc aggctacatt gtgctgacgg cgggcggcgg
    12241 cggtgggggc gattcttttg gctctgcgtt cctgccggcg gtgtatcaag gcacgctggt
    12301 tccttacacg cagtcggatc cgaacaagca aatccggtac ctggcgaatg cccacatgaa
    12361 tctcgcccaa cagcgggagc agctcgacct gctccgcgac ttgaaccaaa tgtacgtgga
    12421 tcagttgcag ggcgcaccgg aactggaggc gacgatccaa tccatggagg tcgccttccg
    12481 gatgcaaacc gaggcgcccg aggttttcga cacgcgaaag gaaagcccgg cgacgctcgc
    12541 caaatatgga gaggacggca tcgggcgagc ctgcgtgacc gcccggcggc tggtggaaaa
    12601 aggagtgcga tttgtgcaga tcgttcacgc cggctgggat caccacgccg acatcatggg
    12661 ccacaagcac accgcgcggg ccgttgatca gccgatcgcc gctctggtgc aggatttgaa
    12721 ggaccgcggt cttctccacg acactctcgt cctggtgacg agcgagttcg gccgaacgcc
    12781 tgtcttaaat ctcggcggat ttcgttcggt gcacaacggc cgcgatcaca atatttatgg
    12841 ttttacgatc ttgatggccg gcggcggcgc caggcccggc acaatctatg gcgccaccga
    12901 cgactttgga ttcaaagtcg cccaaaaccc cgtgcacgta catgacctgc acgcgaccat
    12961 cctgtacttg ctcgggatgg atcatgaaag attgacttac caatatgccg ggcgcaactt
    13021 ccgtctcacg gacgtcgaag gccgggtcgt caaggatatc gtggcgtaac aacccggctt
    13081 gcggcgcgcc agcgcaggag agcaggcgcg ctagaaaaac gcaatcccgc gtccgctgaa
    13141 gatcagcgcc agccataaca gcaaagacgc gtacgcactc agccgcgcgc ttcgtgactt
    13201 cactggccct ttttccgctg cggcaagccc cgtgtaggca gttcttcgga acatcagggt
    13261 attcaaaaca gcgaacgaaa acagcaccat cttgatgcgg aaactgaggc tcttataaca
    13321 gacgacgggt tccgcccacc acagcaggga gccgctgaga acgataatgg cgaatcccgc
    13381 ccacttccag cgccgaaact gcgaccacac ttccgagaca ggctcgtgcc gcaaaccgat
    13441 ccccagcacg ctcaggtcac tcatcagcag catgccgccg aaccaggcga tgcccagcag
    13501 atggaacgcc ccaaggatcg ggtagtagag agctccctcg cgtatggcta tgctcaaaga
    13561 agtggtttcg atccattggc agacggctag aaaagacacg atccaacccc ttctaagctc
    13621 gcggctcgcg cagcccgccg caagcacaga ctggcgaggc ctcgaatttc atggagccgg
    13681 tgcgctgcgc tcttcctctg actgccgccg ggcgcagcct gggctgggcc gaacaggcac
    13741 gcgcagcccg acagtcccga tatgttccct catcccctcg gcaagggacg cgctgtttag
    13801 aagaatgcaa tggcacgccc tccgagaacc acgccggtcc acagggccag cgagagagct
    13861 ccagctattt ttccggccac tgtcgtttcg gaaggctggt cgcggcgcgt aacgcggcga
    13921 tgaatcgtaa agtgggtcag caaagccagc agcaaacacg acattttgaa ctcgaacgca
    13981 ggattgccat agcccctttc cggttccgca gagagcatca gcgggcccgt cgtcagcatg
    14041 aggccgagac cggccagcgt ccagcccttg agatcgccgg ccacttccgg cgccgggcga
    14101 tttctcagaa ccagcccgag aaggcgaaaa tcgacgatag cgacggtccc gattaaaaca
    14161 gccagccccg taatatgcaa ggattcaatg atgggaaata gccactcgga atcgttgatc
    14221 gagttcgcga ccggcgtatt gccgagccac cggaaaaaat gcagaagcag cgtttctacg
    14281 gacatcgtga ttcacctggg ccgcttgagg cgcaactgcc ggcaagtaaa ttcttaagcg
    14341 cggatgccgg cagggatccc atctgcttgt cgggcagcgc gtttcttgac atggaaatca
    14401 attgaatata ctcttaaaaa cgccaaaagg ggtaggagcg cgcggacggg cgctcgaagc
    14461 ggcgctgtga ggcctgtccg agagggaggt caaatccggt gcgaaagcgg ttccaaggtt
    14521 tggtgatcgg gatacccgcc gcagcggctg ccctcctgct ggccgcgata tccgccttcg
    14581 ggcagtcttc ggcggtgtac cgggcgccgc gcacctcgga tggcaagccg gacctgaacg
    14641 gcatctggca ggctctcaac accgccaact gggatctgca aggtcatgcg gcgcggccgg
    14701 gaccggtggt tgctctgggg gcgatcggcg cgacaccggc cggactcggc gtggtcgagg
    14761 gggacgaaat cccctacttg ccttcggctt tggcaaaaaa gagagagaat ttcgagcatc
    14821 ggctcaccgc cgacccggaa gtgaaatgct acctgccggg cgtccctcgg gccacgtata
    14881 tgccgtttcc gttccagatt gtgcagaccc caaaatacat cctgatcgca tacgaatacg
    14941 ccagcgccag ccggattatt tacatggaga atgcgccgcc gagcccggtg gatacctgga
    15001 tggggaactc caccggccac tgggaggggg atacgctggt tgtagatgtg accagtttca
    15061 atgatcagac ctggtttgac cgggccggca attttcacag cgacgctctg catgtgattg
    15121 agcgctacac gccgctgacc catgacgcct tgagctacga ggcgaccatc gaggatccga
    15181 aggttttttc gcggccgtgg aagatacgca tgccgctgta ccgccgcctc gagagcaacg
    15241 cgcagctttt ggagttcaag tgcgtggagt ttgttgaaga actcatgtac ggcccccttc
    15301 ggaagcagcc gagcaagtaa caggaggtta cctatggggt atcgctctct gagagcaatc
    15361 agcgccctgg cgctggtact gggcggcctg ggaatccctc cgcccgccgc ttgccaaacg
    15421 ccggccgcgc ccggaacgac gcaagtcgcc gccaataggg cgcccgcggc ggccacttgg
    15481 accttgccgc gtacgccgga cggccagccg gacctccagg gcctttggac caacgccact
    15541 ctgacgccct ttgagcggcc caaagagttc gccggcaaag aattctttac ggagcaagag
    15601 gcggttgagt ttgcccggcg cgtcctcgac aacgcgaacc gggaccgccg gggaactaca
    15661 cccgaggcag acgtggccgg ctcttataac gagtcgtggt tcgaccgcgg cacagccgtc
    15721 gcgcccacgc ggcgaacttc gatcgtgatc gacccgccgg atggacgggt tccgccgctg
    15781 acgcccgagg cgcgaaagac gatggccgct ctggcagcga tccggcggcg tccgcccgaa
    15841 ggtcccgagg acctgggctt gcaggaccgc tgcatcatct ggccaaccgc cggacctccc
    15901 atggtgccgg ccggctacaa caacaactat ctgattttgc aaacccctca atatgtggcg
    15961 attctcatcg aaatgatcca cgacgtgcgc atcatcccgc ttgatggccg ccctcacctc
    16021 ccgcaaagca tacgccaatg gatgggagat tcccgcgggc attgggaggg ggacacgctg
    16081 gtagtggata ccactaattt taccgacaag acgcatttcc gaggttcgga ccagaatttg
    16141 cacctgaccg aacgtttccg gcgcacgggg cccgacacaa tcgaataccg gttcactgtg
    16201 gatgatccga cggcgttcac gcggccttgg accggggaga tttccatgct gaagactccg
    16261 gggcccattt acgagtatgc gtgccacgaa gggaattatg cgatgacgaa cgtcctcagc
    16321 ggcgctcgcg cgcaagaaaa ggctgcggcc gaagcggcca agaagggttc caagtaggtt
    16381 gtggtcaacg ccgctcgccg gctcaatgga aacaacgtgg cgttcgagcc aaacgttttc
    16441 atccgcggac ggagcatcgt gcgcgcccgc gggcaggcgg ttgccagtct ttcgcaagaa
    16501 ataagaagaa ggagggagtc tcgtgcgaac aaagctagcc attgtggtag ccgcagccgg
    16561 gctgcttatg accgcagggc cggcgtggtc gcaccacgca ttcgcagcgg aatttgacgc
    16621 gaaaaggccc gtcaagctgc gcggaaagat cactaaaatg gagtggatca atccccacgc
    16681 ctggattcat atggaggtaa tcggcccgga cggtaagccc gtcaactgga tggtggaagc
    16741 cggaacgcca aacacccttt tccgccgggg ctttacaaag gattctctga agcccggaac
    16801 ggagattctc gtggacggct accaggccaa ggacggatcg aaccgggcca acgggcggga
    16861 tgtgactttc cccgacggga ggaaactctt cctcggctcc tcgggcacgg gcgccccgta
    16921 cgacaacgag aggaaatgaa acgccgcact gcttccgccc tgttggcggc aggcagcgac
    16981 gcgagcgccg gcgcgatatc gtagtcgtca taccgatctg cggagatagc cggtgaggac
    17041 gttcaaggct tcgaagcggg aaaccccacg ccgcaagcgg cgccggaaac cagcaatcca
    17101 atcggcggcc ggtgggcttg ctttcaagag aacccgatcc accgcccgcc gattcccact
    17161 ccacaccaga gtgccagtga gatcaggccg accagcttgc tccgtatcgg cgccaccggc
    17221 gccccatccg ccagcgtcac tttgcggcgc acagtgaagg tgaagagaat tgccagggcc
    17281 aggcaggtca tcttgaacca aaacgcctca tggtaatagc atttgatggc ctcggaggtg
    17341 aaaagcagga tgccggaagc gatcatcacc atcagactgc cgatcagcca gcgctgcgcg
    17401 tctatggcaa gctgcgcaac cggctggcgc cgcaatccga agccgagcag gcgcaggtca
    17461 acgaccaaaa cagcgccccc gattacggcc agcgccagaa ggtggaaaga ttcgataacc
    17521 ggaaacagcc acgtcgagct gcggatcgcc tcgcccacgg ccgagttttc gcaccactgg
    17581 aagaaaggca agagagacat tgcaaatatc actcggcggc tcgtccctgc ggagatggcg
    17641 ggaaaagtgg acgcggctgc ggattgcgcg ccttgctgga cgccgcttca ctcgtagtcg
    17701 cggcgatggg acctgagtcg aaccagttat aagcaatcat gcgcccgcaa atgacaatgc
    17761 cggcccaaag caggagagaa aggccggcgg caatgcgggc tcgtgtggga ggaactggat
    17821 ccagatccca ttccttcacc ttgcgataca cgcccgcgtg aaagacccag gcgtttaatc
    17881 cggagagcac caacatcgca aacttgatgc gaaagaagat attctgaaag gtcctcaccg
    17941 gaatcgcgta aaacaaaagg atcccgctcg cgaccatcac cgcaaagccg gcggttgtcc
    18001 agggcagcag ccggtctaca acctccgaga cgggggcgcg tctcatcgtc aggcccaata
    18061 ggcgcaggtc cagcatgacg gccgtgccca gaaacaggca caaggtcagg acatgtacgg
    18121 actccacgat gggatacccc caggtcgatt cgtggagagc gatgctccac tgtgtgtttg
    18181 ccagccactc gcagaaacga agcacagaca tttccagccg agatcccggc cctggaaaat
    18241 cgtatcgtga atcggcggac ctggcaatgc cgccggccga agcgccgttc gccgcaggga
    18301 ggagtgagcg ctatgtggcc ctaacaacgg aactgaggcc gatcccttgt tcgattaaaa
    18361 caaaacttcg gttgttattt ctcaggccgg cgccttcagg tctcctcttt ttgccgcctg
    18421 gatgatgtac tcggagcagc ggtaagcaag cgcctcaatg gtgcaagtgg ggtggttgcg
    18481 cccgatcgtg acgaaactgc ttccgtccac gataaacaag ttcggcacgt cgtgcgccct
    18541 gtgaaacttg tccacgacgg aagtcttcgg gtcgttgccc atgcggcacg tgcccatcag
    18601 gtgcccgccg ctcctggatt ccgttaccgg agacgcccat ttcttaatcg cccccgccgc
    18661 atccaggatc tccagggcgc ggtccacgaa aaaccgcttc gtcttcagag tgtccggatg
    18721 ctcccggtat gtgacgcgca tggccggcaa tccccaggcg tccttcacgt ccgggtccag
    18781 ggtgatcgtg ttggtctcga gcggcaagga agtgcagaag gcctggatgg tcatcgtgcg
    18841 gctgaagtat tccttgcacg cctgcttgaa tcccgcgccc cagcgcgggg cgtcgggcgg
    18901 caagccctgg agcgcgtagc cgatgggacc catatagccc caccgggcgt ccagccgccc
    18961 gccgccatag aaaccgcgtt tcgggtcgga atcgtaaaaa tcctcgatca tccggctgga
    19021 ctgcacgctc ttgtattcgt tcagcggctg ctcgaaggtc ccgtgtgcgc tggcgccggc
    19081 gccgaaattc aaaaattttc ccaccaggcc gctggaattg gccagcccgt caggaaacag
    19141 gctcgatttc gagagcagca gcaggcgcgg cgtctcggtc ccgttggcgc ataacaccac
    19201 cgctttcgct ctctggaaat tttccttctt ttccgcgtcg aaatacacgg cgcccgtcac
    19261 ccgcccgctt ttgtccacgg aaatctcgcg gacataggaa ttcgggcgaa tctcgcaccg
    19321 ccccgtcttc tccgcgatgg gaatcacggt ggaaagcgtg ctcgatttgg cgccgatttc
    19381 gcacccgaac cactcgcaga agccgcaatt attgcaggct ttgcggcccc gatagggctg
    19441 ggagagaatg gccagcgcca ccggcgaagc atgcaagccc agtttcttcg ctgcgcgctc
    19501 catcaagacg ccggaggatt ttatcggcag cggcggcacc ggatagggtt tcgaccgcgg
    19561 cgattcaaac gggctcccgc cgcccaggcc ggaaacgccc agttcccatt ccactttggt
    19621 gtagtagggc tccagttcgg cgtaggtgat cggccagtct gccaggcctg tgccgccgat
    19681 cggaccccag cgcgtcctct caatgaaatc cacttcgtga aaccgggatt gcgaagccgt
    19741 atagtgaaca gtcccgcctc cgacgcaccg gccgtaactg acggccggcg ccaaaaccgc
    19801 tttttccttc tcgcttcggc ggaatgtgtt cggctggagc gcgtggtcat tcaccagtgc
    19861 gggcgtgctc cggattttca gctcgtcgtg ggaaaaatcc ttttcccgca ggtaggggcc
    19921 ctgctccaaa acgaccacct gaaagccggc ggaggaaagc tccttggcca ctacgccccc
    19981 ggcgcctccc gctccaatga caacgaaatc gacctgagtg ctcggtttga acttcgtcat
    20041 gcgattttct ccggcccttc ccggcagctt tatgcgcctt tgtagtcccg gtcgtaatag
    20101 ccgaacggag gttgaaaatg gaacgcatca tcgaagccga tcagcctcca accggttttc
    20161 tcgtagttgc cgccatattc cggattggcg aagaatccca caatggtgtg cgtccggatc
    20221 atcccgaaga acggggtttt ttcgatggat ttgagcaatt caatctgctg cgcggaagtc
    20281 agagccgaga acttggccac gcccgggaac atcgtcgccg tcttggcctg aagatcccgc
    20341 agcccttcgg tgtagacggg ctgtttgtcg cggtcgaatg tatgcagcgc ccgatcaatg
    20401 aagtggatga ttcgcgcttc gcgcgctccc ggcgagtcat cactgggaat gatctgcgcg
    20461 gccacggctt ccacctctgc ggcctgctcc ggcgagaaga actcaaattt tccggccgct
    20521 cctgaccggg ccagccgatg gacatgctcc tgggcggcca cgatcccagg ccaacttgcg
    20581 gccagccaag ccgagacgga gccggcgaga aaatcacggc gcgaagcccc ccgctttttc
    20641 atgctccctc cgtttgcgcg tccaagtagt tctggtcagg gactatatca cgcggagttt
    20701 tcgatttcaa catacagatg gctttttgcc tgcaagttcc cgctatagcg cgcttctggc
    20761 agtcggcccg ctggcggaga gcgtcccagt tacatcatcc caaaaccctt gcatatttct
    20821 tgtctaggct ctcctcgctg tgaggttgca ggcggcggcg ccgctgcctc cgccattgtc
    20881 gcggtgcaaa acaacaggaa aaggtgcaag ccgaatacgc aggctttctc gggaattgaa
    20941 cgcaatatga cagaagaatg gaatcgaatg cgggcggccc ggcgccaagt gtttacaact
    21001 gccgggccag cccggagttt aaactgaaaa ttaagacggc tgttagcaag caaatccggc
    21061 ttggcggcac ggggcgcttt gcgggtaggt gttggatttt ctcgaagcgc ggcctgctcg
    21121 tcccgtccca gcagggccgc aaaaagtgga gagccgcgcg ccggccttat gcgcaagatt
    21181 attttgtttc tgccgcctta ctcgggtccc gtgttcggcc ccccgctggc gctgctcgcc
    21241 ctggcagcgc cgcttcggga agccggctat gaaccgaaga tcattgatgg ggtaatcacg
    21301 cctgattacc ccggtgtgct gcgggcggag attgcgaacg cgctttgctt tggcgtctcc
    21361 ttgctgactg ggccgatgat ctcgcacgcc gcgcgggcgg ctcgccttgt gaagcagctt
    21421 cgtccggata ttccggtcgt atttggcggc tggcacccta gcctgctgcc ggagcagacc
    21481 ttgcgcgaag attttgtcga tgtcgtggtc cgccatcagg gcgaaatcac gtttctggaa
    21541 gtggtccgca ggctggaaag ccggcagcct ctcgatttag tctccgggtg ctcgttcaag
    21601 cggggcggga aaatctgtca gaatcccgac cggccggttg ctgcgctcag ttccttgccc
    21661 gcgccgttgt ttgaggcggc cgattttgac gcttacgaga aggtctccgg agagcgtgcc
    21721 gcgccatatg tcacgagcgt cggctgtcct tacgcttgcg attactgcac ggacacggtc
    21781 ttttacaagc ggcgtttcaa tgctcttccg gccgagaggg tcgtcaacga aatgacgggt
    21841 ctggtccgcc gtcaccggct caccaagatc gcgctgtgcg attcgaattt tctggttgac
    21901 gcgcggcgcg cgctcgaaat tgcacgcggc attatggcgt caggcctgcg tttcaggtgg
    21961 tcgttccagg cctccaccga tctgctctgc cgcatgagcg acgacgaggt gcgcctgctt
    22021 ggcgaaagcg gagtggaaca tatcggcttc ggtacagaat cggcttcgga agatgtattg
    22081 cgccagatga ataagctcca tcagcgggtc cctgatatgt tcgagacggc ccgtaagtgc
    22141 cgccaggcgg gcatccgtgc gacgttcaac ctcattttgg cttacccggg ggagacggaa
    22201 gcggaccggg aagtgacttt gcgggtcatg agcgacattg gccgccgcta cggaaatgtg
    22261 accttttctc ccaacatctt cacgccgtat ccgggcatcc cgatttggcc ggaactgcgg
    22321 cgtatgggtg tttccgagcc gcgatcactc gaggcgtggt caagcatttc tctcgggcgg
    22381 aacgtcctcc catggcttca ggggaggccg tatcgcagtc tgaagcggaa cgtttccttc
    22441 tttctcctga cgaatcagat ttcacgggcc gcccgggagc ccggatcttc cgtgcgccgc
    22501 ctgattttgc gcattgtgag cgtgttgttg aactggcgag tgcgattcgg atttttccac
    22561 ttcccgctcg acctctggct ggctcatgct acaaaacgcc tggcggcgcg ccggtcgctc
    22621 ctaacgggcc gcccgctggc cggcagcctc aatgaggcgt gttgacgcgg cccacagagg
    22681 tgggaaaccg atttacgact tgcccttgcc ccggccggcc gtcctaaaga tctgccccga
    22741 tatgcttttc tggcggcttt tgcgcgcggt tccggcaagc agcggttttg ccggatcttc
    22801 aatacgattt acggctgtgt cgcggttata ggggaggccg ctgccatccc ccaaagtccg
    22861 cttcgagttt tcgcgccacg gcgagagcga cgtcttcccg ccagggccgc ccggcgattt
    22921 gcactcctag gggcaatccg caggcggaag ttccgcagcg cacgaccacg gccggccagc
    22981 cggtcaggtt gaagctcatc gtataggtaa atgcctccag atgatcgaat gtcgtcccgt
    23041 gaggcatggc ggcatcggcg caaaccggac agagaagcag atcatgcttt tcgaggaagg
    23101 aaagcatcgc gctgcggaac ctgtcgacgc ggatcagcag gcggccgaac tcgggcgctg
    23161 cgagcggccg ctgacccagg atgctcagga accgctccag caacggatgc gcttgctggc
    23221 tgccgcaggc ttgcagcagt tcccgcaagc aggctccggc gtcggcgccg aagagtccga
    23281 gcgccagttc gtaggtctgc tcaattcccg ccggcctggc ttcctcgatg cggactcccg
    23341 agtgagcgag cgaagccgcg gctttttcca ccgcggcgac agtttccgga gtagctggca
    23401 gaatgccgtt gtcggtgtag aaggcggccc gcaggccgcg aaggtccacg gcgttcgggt
    23461 cgcgccaagg catgggaacg acggcgggat cgacccaatc tgcgccgctg aggataggca
    23521 ttgccagtat taagtcttcg acgaagcgcg ccatggggcc gatttgccag agagcgtcaa
    23581 acaggccgcc cggcggagga aaatggccgg ttcgggcaag ccggccgctg gtgggcttga
    23641 tgccggcgat tccgcagaag tgggacggca gccggatgct tccggccgca tcgctgccca
    23701 ctccgagcgg cgattccccg gctgctatgg ccgctgcttc gccgccgctg ctgccccctg
    23761 cggttcggga aaggtcgtac ggattattgg tgcgcccata aatcaggttg tctgactcaa
    23821 aggcgaacaa aagatcgggg agattggttt ttcccagcgg aatagcgccg gcgccgcgca
    23881 ggcggatcac agcggttgca tccgccgtcg acacgaattt cgatcgcccg agagtgccgg
    23941 atgtgcaggt catgccggcg gcttccatcg agtccttaac agttatgggc acgccgtgca
    24001 ggggcccgcg aatcattcct ttggcgaagt cctcgtccgc acggcgggcg gcctcgagcg
    24061 cctcctccgc cgccagttgg accacggcgt ttagtttcgg attcaccgcc tcgatgcgct
    24121 tcaggtgcat ttgcacgagt tcccgcgagc tcacctcgcc ggtgcggatc tcgcgcgcct
    24181 gggcggttgc cgaagcgtag atcaattccc ccatgctgta cgctcgcgaa gctcaacggg
    24241 gcggcgggcg gtagcctcga tcagatccag gtacacttgg acggcccgtt tcaagctgtg
    24301 gttctcggcg atatgggcgg cggcccggcg gccgatttcg gcgcgcatct ccggattttg
    24361 ctccaaagcg cgcagataat ggcccagcat ctcgacttcc gactcgtccg ggtcgattcg
    24421 aatcacagac agctccggaa aacgggaggt ctcctcgctg gccgtcacca gcaccggctt
    24481 gccgattccc atcatcttga tgccgattcc cgaagt
//
LOCUS       DASREK010000137        28938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_403500, whole genome shotgun sequence.
ACCESSION   DASREK010000137 DASREK010000000
VERSION     DASREK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 28938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28938
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403500"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /gene="rho"
                     /locus_tag="VFA54_06860"
     CDS             complement(<1..765)
                     /gene="rho"
                     /locus_tag="VFA54_06860"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZS50560.1"
                     /translation="MDLAKLKEMNITKLNQVAKELGVVGAAGMRKQDLIFKILQAQAE
                     KSGQIFSEGVLETLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRRFDLRTGDTVSGHVR
                     PPKEGERYFALIKVEAINFEPPEEGRNKIFFDNLTPLYPSGRIRLETTKDNLSGRVMD
                     LLTPIGKGQRGLIVAPPRTGKTMLLQSIANSITTNHPEITLIVLLIDERPEEVTDMQR
                     SVKGEVISSTFDEPAARHVQVAEMVIEKAKRLVEHKR"
     gene            complement(918..3323)
                     /gene="lon"
                     /locus_tag="VFA54_06865"
     CDS             complement(918..3323)
                     /gene="lon"
                     /locus_tag="VFA54_06865"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZS50561.1"
                     /translation="MQTTREKSETKRLPMMPIRDVVIFPFMMTPFVVGRESSVRALEE
                     ALAGDKKIFLATQHDASVDEPRPNEIYSVGTIVNIVQSLKLPDGNIKVLVEGVERAKV
                     VSVSEDEGFFRAIVKTTSYKVENGQQLEALIGRVTSLFEQYVKLSQNLNYETMIAAVR
                     VDDPGKLADTIGANLQLTIEEKQDLLEIFDPIDRLTRVADVLDIEIEKLNMDRTIQGR
                     VKRQMEKAQKEYYLNEKIKAIQKELGRGEKSEFDELRKKIESAGMTKDAQEKALAELK
                     RLENMPPMSAESTVSRNYLDWLLAVPWKKKSKEIRDLSYAEKVLESEHYGLEKIKERI
                     LEFLAVRRLVKNPRGSILCFVGPPGVGKTSLGMSIAKATGRKFVRLSLGGVRDEAEIR
                     GHRRTYIGALPGQIIQMMKKAGMRNPVFMLDEVDKMSTDFRGDPSAALMEVLDPEQNY
                     MFVDHYLDVEYDLSKVFFICTANVLHTIPPALQDRMEVIRLSGYTEDEKVEIATRFLV
                     KRQREETGLTEKQLTFTREGLVRIIQEYTRESGVRNLEREIGNVCRKVARRAVKDPKQ
                     EISITIDAENVPEFLGVPKFRDLQSEKKNEIGTAVGLAWTEVGGQILNVETTLMEGKG
                     KLMLTGKLGDVMQESAQAAMSYVRSRSHQLGLPRDFYRNLDVHIHVPEGAIPKDGPSA
                     GITIATTLVSALTKIPVRGDVAMTGEITLRGKVLPIGGLKEKLLAAHRQGIFEAVLPK
                     DNEKDLPDIPENIRTAMKLHFVENMDQVLQIALERPLELAVPMAPAVELASQPAAEEN
                     VTH"
     gene            complement(3347..4597)
                     /gene="clpX"
                     /locus_tag="VFA54_06870"
     CDS             complement(3347..4597)
                     /gene="clpX"
                     /locus_tag="VFA54_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZS50562.1"
                     /translation="MRCSFCHKSQEVVGKLISTPSDYPKAYICDECVAVCNSILEDDK
                     AERVPVGAPRLQRPQEIKELLDQYVVGQDGPKKKLAVAVYNHYKRISMNRQRMGDVEL
                     AKSNILLIGPTGTGKTLLAQTLAKILDVPFAIVDATTLTEAGYVGEDVENIILKLLQA
                     ADGDVQKTQQGIIYIDEIDKIGRKDENPSITRDVSGEGVQQALLKILEGTIANVPPQG
                     GRKHPHQEFTPVDTTNILFVCGGAFVGLEKVIGRRVGKKSVGFRSPHAAEEEKARRAS
                     ADGARDIDLLEQVQPHDLIKFGFIPEFVGRLPVVAVLSDLDQQALVQILTKPRNAIVR
                     QYQKLFEFENVRLRFEDDAVEAIADLALERKVGARGLRMILEEMMLDLMYYLPSYKKV
                     REFVVTREMVLNHDLNLNLLEKAG"
     gene            complement(4781..5383)
                     /gene="clpP"
                     /locus_tag="VFA54_06875"
     CDS             complement(4781..5383)
                     /gene="clpP"
                     /locus_tag="VFA54_06875"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZS50563.1"
                     /translation="MSTVPHLGLIPMVVEQTNRGERAYDIYSRLLRDNIIFLGFPVDD
                     NVANLITAQLLYLEAEDPEKDISLYINSPGGSITAGMAIYDTMQFIRPDITTYCIGQA
                     ASMAALLLAAGTKGKRFALPNSRMLIHQPSMGGLSGQATDIDIQAREILRMREITNEI
                     LAKHTGQPLEKIQRDVERDFIMNAAQAKEYGLVDEIIVKH"
     gene            complement(5431..6693)
                     /gene="tig"
                     /locus_tag="VFA54_06880"
     CDS             complement(5431..6693)
                     /gene="tig"
                     /locus_tag="VFA54_06880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZS50564.1"
                     /translation="MPLVAGCKHELEITVPVEAVEQENERVITKIRNRARIPGFRPGK
                     APASMVRLRFADEIRREVMEALIPRHLYKQIEAEGLHPVGTPDVSNVKFEPGQPLTFK
                     AEFEVLPDFELKEYRGLPVAYDEPEVTEEEIAAALEQIRERHAIYRNLDPRPLADGDI
                     AVVSLRSTQTPAGMAPIHQDELTVEIGGEDTLAPFTENLRGLSPGDQREIQVTYPEDY
                     GEPKLAGQTVAFHAEVRGLRKKELPELNDELAKDEGDFRDLAELRERIRSELQAQKRE
                     EAHRKAKAKLIEDLVDAHDFPVPEALVEKQIHSNVERSLRSLADRGVDPTKLRLDWKK
                     IQEVEQPRSIREIKAGLILDKIAAQERIQATQEEVDRQVQLYARQTGQPVAVARAKLA
                     ESGALDRIASQIRSEKTLQFLFEQARKS"
     gene            complement(6782..8338)
                     /locus_tag="VFA54_06885"
     CDS             complement(6782..8338)
                     /locus_tag="VFA54_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS50565.1"
                     /translation="MRGAGLHLRKPPIVCFAAGFLFLLITHIRFLDLPYHWDELGYFV
                     PAAHDLLHWHSLIPRSTLPNVHPPLLMLYLAGVWMLFGFQIPATRVAMLGIGAATLAA
                     IFALARRLSGSTRAACAAAFLAALSPPFVIQSMLAQLDLAAALWCLLSLYFFLERRPW
                     RCGAAATALVLTKETGLLVPMALIAFSLHAFWQNQKGVWNSGTPKGMAGAPAPVRAWC
                     SASFPILPLLPPFGAIAVWLLFLRSKTGHWFGNSEFAAYNLARAFDGLRILLVFLRRC
                     YQLGISDFHWIGSALLALAILRYGALRGRRWKLVLTVIAGYVLLHSAVGGAVLLRYLL
                     PAVALFYIAVAAASELLPAALRRAGLGTLAAGLVISNWWNPPYPFSYEDNLAVVDFIR
                     LQQRAAQWLTIHSGDQTVTTAWPLTDALVNPLGGYVRRPLKVNAVENFQPEALRRVSP
                     EAVQIFVLYSRSWDPPGGVQGWPPVAELLRKYFGYTPQITSDSLEESLHLRRIAGWQM
                     RGQWMEILGR"
     gene            complement(8367..8660)
                     /locus_tag="VFA54_06890"
     CDS             complement(8367..8660)
                     /locus_tag="VFA54_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HZS50566.1"
                     /translation="MTQPQSVKIKVVRRADFRENYANSVQMRVNLWDFYLEFGTVSQT
                     DPEVVNIETFQGIYLSPQQAKALLNVLQQNVTQYENTFGELKLDAKAAMGVVQ"
     gene            complement(8717..9145)
                     /gene="nusB"
                     /locus_tag="VFA54_06895"
     CDS             complement(8717..9145)
                     /gene="nusB"
                     /locus_tag="VFA54_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZS50567.1"
                     /translation="MASRHESRERALQMLFQWDQSRESPEQVIAGYWGGLFSEEMHGE
                     ESPRRKPVDEFANELLRGAVKRVAEADALIRQYAEHWRLERMSAVDRNVLRLAIFELL
                     QRQTPPPVVIDEALEIARRFSGEESVQFVNGVLDAVRKAL"
     gene            complement(9147..9635)
                     /gene="ribE"
                     /locus_tag="VFA54_06900"
     CDS             complement(9147..9635)
                     /gene="ribE"
                     /locus_tag="VFA54_06900"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZS50568.1"
                     /translation="MNQNIVTVEGKLEAAGLKFAIAVSRFNSFITERLLAGAVDGLER
                     SGCDPKSIEVVRVPGSRELPLIARELGRSGQYDAVICLGAVIRGETTHYDYVAQEASK
                     GIAQAAMETGIPVIFGVLTCDTLEQAIDRAGAKSGNKGFEAALTAIEMANLMKQVRAK
                     KK"
     gene            complement(9737..10561)
                     /locus_tag="VFA54_06905"
     CDS             complement(9737..10561)
                     /locus_tag="VFA54_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZS50569.1"
                     /translation="MACNLSPQLICCLAMEYQNLLYEVEHSIALVTVNRPEKYNALNR
                     GTILDLERCVSEIERDPAVRVAVFTGAGEKAFVAGADINELAQLRPLEAREYSSRGQR
                     LFSRIESLPKPTIAAINGFALGGGLELAMACSIRIASENARLGQPEVKLGIVPGYGGT
                     QRLPRLVGKAAALVVLLTGDPISASEAYRIGLVQQIVPQGELLAAARQWAARMLAAAP
                     MAVSMCLNAVNAGLEMPLDSALALEAAAFSLCCGTEDMREGTRAFLEKREARFRGA"
     gene            10670..12493
                     /gene="lepA"
                     /locus_tag="VFA54_06910"
     CDS             10670..12493
                     /gene="lepA"
                     /locus_tag="VFA54_06910"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZS50570.1"
                     /translation="MKAVPIDRSLIRNFSIIAHIDHGKSTLADRLLEATGALEQREMM
                     EQVLDSMDLERERGITIKAHAVRLMYKADDGQTYQLNLIDTPGHVDFSYEVSRSLAAC
                     EGALLVVDASQGVEAQTLANTYLALHHNLEIIPVINKIDLPAAEPGRIREQIENIIGL
                     SAQDALLVSAKQGVGIHETLEAVVKRVPPPSGSPEAPLRALLFDSWFDAYRGVILLIR
                     VVEGRLRLGAHIRMWSSGRVYEVEGLGYRTPKSYSCEELSAGEVGFVVANIKHVAETR
                     IGDTILDDDNPAEEPLPGFEESKPMVFAGLYPVEAHAHGELREALEKLQLNDSSLRFE
                     PESSGALGFGFRCGFLGLLHLEIVQERLEREFGLNLITTAPSVSYRVTTRSGDVIEVD
                     NPMKFPPPAEIATIEEPVLSVTIITRDEYLGGILKLLEEKRGVQKGFEYVGANRVLLT
                     YEVPLNEVVLDFYDRLKSVSRGYASLDYHFAGYWNSPLVKLDIMVAGETVDALSLIVH
                     RDQAYERGKALIEKMRKLIPRQMFEVALQAAIGNKIIARENIAALRKNVLAKCYGGDI
                     TRKRKLLEKQKEGKRRMKRVGRVDIPQEAFLAVLKVGEESD"
     gene            complement(12566..12766)
                     /locus_tag="VFA54_06915"
     CDS             complement(12566..12766)
                     /locus_tag="VFA54_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50571.1"
                     /translation="MEFRYGICPHSLMRWKMFERIQELRVERGAEVGGSLMRFLVDAE
                     LAAIARATVERERRAAEVTHRK"
     gene            complement(13533..14378)
                     /locus_tag="VFA54_06920"
     CDS             complement(13533..14378)
                     /locus_tag="VFA54_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50572.1"
                     /translation="MLGQVLMSNYQPAPRVLAYIMSPCRIFQQRGSFVFWGFRTPLVG
                     LILACVIAPAPARAVEDDLPADRAGMEPAAVVGEKPPADQHILKVIPNYKTVEDPSQP
                     VEPMPIREKFKLGAKDSFDPHALPVIAIYAGIEQLQNQYKSFGQGMEGFGKRYAGSYA
                     DQAIGNMLGEAVFPSVLHEDPRYFRKGRGNFFARAGYAMSRVLVTRTDAGSQQFNFSE
                     FLGNGAQAAISNVYYPAPDRTLHQTAYKAISQTVLDSLFNIAKEYWPDVRQRLFRKSN
                     PYPAP"
     gene            14561..15535
                     /locus_tag="VFA54_06925"
     CDS             14561..15535
                     /locus_tag="VFA54_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZS50573.1"
                     /translation="MAYHWDELTPFEALPDRGEGVKVAVPEKILKLWGNFQMPKPPSE
                     ARAAVSFNFVQTLDGVASYGVRGFAGGGAISKKREDRWMMDAYRTMTDAIVLGAETVR
                     AEDREIKRRREAGGRPLYEIDGMPTNGFVATVEDPELAEWRRRTRGERHPVLVVITAS
                     GELPEDLGIFDLASQRILIFTTPAGESVLIEMMRNKRRFANVETIPFGGIPADLPLFL
                     TGILKQKFGIRYALCEGGPATAGAFLQDGLDDELLLTVAPLLATGKDEGGQARKRIVE
                     GPDFYPAAAFDAPGLRIPPGARRSPEYLLHSVRRHGDHMFFRYWRQRS"
     gene            15678..16853
                     /locus_tag="VFA54_06930"
     CDS             15678..16853
                     /locus_tag="VFA54_06930"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZS50574.1"
                     /translation="MVVGVPKETFPGERRVALVPGVVPTLTKAGFEVLIEAGAGVQAG
                     YSDAEYEGKGAKIVSTRAEVFAADIVAQVLCYGSNDKTGKADLPLLRRNQVLVGFLRP
                     LGSIDTIQEIADTGVTAFAVELVPRITRAQSMDALSSMGTICGYKAVLLAADTLPRIF
                     PMLTTAAGTITPARVFVIGAGVAGLQAIATARRLGAIAFAYDLRPAAKEQVQSLGGRF
                     VELPIEAKDAQDARGYGRAQDESFYQRQRELLGRVVSESDVVITAAVVPGKKSPVLVT
                     KEMVACMAPGSVIVDLAAERGGNCELTRTGEIAVEHGVTIIGYINLASMVPYHASQMY
                     ARNIAAFLLHLCKGGQLQIDVTDEITRETLLTRDGEVVNALVRQFFSLPVPAAATSR"
     gene            16887..17180
                     /locus_tag="VFA54_06935"
     CDS             16887..17180
                     /locus_tag="VFA54_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZS50575.1"
                     /translation="MPSDLITSLYVFVLAGFVGFQVIRRVSPLLHTPLMSLTNALDAI
                     AVVGAIVIAGERKSTLSTVLGTLAVVAATSNVVGGFLITDRMLRMFKESRPRK"
     gene            17190..18581
                     /locus_tag="VFA54_06940"
     CDS             17190..18581
                     /locus_tag="VFA54_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZS50576.1"
                     /translation="MSPFLTDQVIEIVYLVTTVLFIFSLQWMSAPSSARRGVLAGEIG
                     MLLAIGGTLLHRGIIDYRPVVIALVAGSIIGVPLGLVHMTAVPQRTALSHAFGAFAVA
                     LVGSAEYYLRAPGVSPFMMSVLAAEVILGSLTFTGSLMAAGKLQEILPQRPITYKGQN
                     VVNLGLLGIAVAIAVELVTHPERKILFPLIIALPLLFGVLMIVPIGGADMPTVISLLN
                     SYAGLSAAAMGFVLDSKLLIVAGALDGSSGFILSVIMSKAMNRSFTNVLFGAFGQVQP
                     SAAAAKQERPVRSATAEEAAAILAAADTVVIVPGYGMAVAQAQHKVRELYDALTKRGV
                     DVKFGIHPVAGRMPGHMNVLLAEADIPYDKLLDMDAVNPDFPQTDVALIIGANDVTNP
                     AARNDPGSPIYGMPILDVDKARTVMVIKRSLSPGFAGVDNPLYYLDKTLMLFGDAKAF
                     VGDIVRELGGEHR"
     gene            complement(18723..18995)
                     /locus_tag="VFA54_06945"
     CDS             complement(18723..18995)
                     /locus_tag="VFA54_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZS50577.1"
                     /translation="MAIVYIPTPLVALTGGNATVEVEGATVRQIIDNLEQSWPGIRER
                     LVAKDHLRPNISVAVDGEVSPLGLLERVSPTSEVHFVAAISGGSRR"
     gene            complement(18997..21282)
                     /locus_tag="VFA54_06950"
     CDS             complement(18997..21282)
                     /locus_tag="VFA54_06950"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZS50578.1"
                     /translation="MKTQTETSTETGIGGREYAVIGTRPIRHDGADKVTGRAQYGADL
                     RLPGLLHGCIVRSPYAHARIRRIDTAKAAALPGVKAAVTAADLPGAGDRIVDLGEGAT
                     PLSYIRGNILAGEKVLYKGHAVAAVAAVSPHVAEEAAGMIEVEYEPLPCVLTAPEAMQ
                     AGAPILHDDLRTEEFGKRAAKASNVAQHFRHSLGDIEKGFREADVIVEREFRTATVHQ
                     GYIEPHNATALWNHDGRVHIWCSTQGAFVVRDTTACILDIPVSQVKVTPMEIGGGFGG
                     KIPVYLEPVAALLSKKTGRPVKIVMSRKDVFEGTGPTPGSYIKVKIGARKDGRITAAQ
                     AYLAYEAGAYPGAMVAPGAMCIFAAYDIENVVIDGFDVVVNKPSTAAYRAPGATNAAF
                     AAETVVDEIAEKLGIDPIDFRLRNAAKEGTRRADGPRHRRIGCAEVLEAMKRHPHYTA
                     PLGGPHRGRGVAIGFWFNVGLPSSCTISVNADGTVSLVEGSTDIGGTRTSVAMQAAEV
                     LGIAAEDVRPTVADTDSVGYTAVTGGSRVTFSTGLAAYDAAQDVVRQMKERAAKLWEI
                     GVEQVRAERGIFCAGDRSISFKELAAHLEETGGPVIGRGAVNPKGVGGSFAGCIADVE
                     VDPETGKVSVLRFTSIQDAGKAIHPSYVEGQMQGGSVQGIGWALNEEYYMSPEGVMLN
                     ASLLDYRMPTSLDLPPIETVIVEVPNPGHPFGARGVGEASIVPPPAALANAIYRAAGV
                     RLNSLPMKPSVVMEAIWNAKA"
     gene            complement(21279..21764)
                     /locus_tag="VFA54_06955"
     CDS             complement(21279..21764)
                     /locus_tag="VFA54_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZS50579.1"
                     /translation="MRARTQVQATINGETVEFLCEPRQSLLECLRDELGLTGAKEGCN
                     DGNCGACTVILDGRIVNSCLVLGLEAAGSEITTIEGIARGAELHPVQRAFLEQAALQC
                     GFCTPGFIVASKALLDEEPDPSPERIRLWLANNLCRCTGYDKIVRAVEQAAAQLQKVK
                     A"
     gene            complement(21770..22636)
                     /locus_tag="VFA54_06960"
     CDS             complement(21770..22636)
                     /locus_tag="VFA54_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZS50580.1"
                     /translation="MKPFDYAAPRSVAEAVRLLGSNPRAQILAGGTDLLVQLRSGRKA
                     ADLVIDVKRIPELNEIRYDPAGGLTLGACVPCCRIYQDRTASRFYPALVEVASLIGGI
                     QIQGRASIGGNLCNAAPSADAVPVLIALRTTCHIAGAAGSREIEVEKFCTGPGRTVLQ
                     PGELLISLHLPPPEPHSGARYLRFIPRNEMDIAVAGAGASVVLEDGRFRAARIALAAV
                     APVPLYVPEAGEWLSGKPVNAENIHRAAEIAKAAAKPITDMRGTAEYRRHLCAVLTRR
                     ALEAAIQRAREA"
     gene            complement(22890..25412)
                     /locus_tag="VFA54_06965"
     CDS             complement(22890..25412)
                     /locus_tag="VFA54_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50581.1"
                     /translation="MATPTLESPVEALKLQYDRERLEDIGYMTCMTLVLLGNYAQTGH
                     FGGPLAYTPFNVAVHLAPPELGGLRYDYRRPKHPYSDKFMLAGGHCIPTCYALWMIMG
                     EALARKYAATRDRRYYVDPSVAVLPVDALGFRRGAGALKNLLQNHGLADHPLFAQAKG
                     RGIRALAGHAETTDVTNDVNGGPSGVGIATAAGKAAFWDFIGAPAGSPKVIAFEGEFA
                     MTEGHAQELKTQALALQVGKRLRILFSFNNAGIDDKLIGGVIAEKFDGYRIIDQWTSY
                     GWNVFSLENGHNYDQIVSALKIMEDWDPRDRRPMIVIANTTKGYWPAAVDGKILDNID
                     QIVSYPSHPYAMKMNSEYFVALAQTFEKHYGVEFEGIRKGPVSDPRERLIQFKTNIDV
                     VMSVLGRNGLGDWLAERLVATGDTVKDNLPLHIDVHRDPFLDDRLRVANLPVEPQKVT
                     VKNRYSGAEKQVSIALFRKPGEVAGTRRAISEIIKWVNYVTDNRFLTIAADLSESINL
                     EHGNLWGHYDPEKNPLGTRLKAAIQEAGNVSTAIGLVGQSASVDPTKFAGVWAISGTY
                     GAFTPLMYLPARVWSQQNQDSRFRMGVLHILAGHSGPETAADARTHFGIFSPQVWKLF
                     PRGQVINLNFWDYNDVAPGYFAAAEIAARDPKVGIITIEVARPDFPVADRSKFADPDL
                     RAAAKGLYVIRDFTPGVPRHGYVISQGSSSTVNLVSTLPRLEAAGINVKVIAAISEDL
                     FDRQPEAYRNSVLPPEAHYDLMVVSTGTRRMWPIRNVGPLTDEYSLTSDWHNRWLTGG
                     LEPDVIAEAHLDAESIFSGIQRFAQEREQRLSRQREMLGKLN"
     gene            complement(25656..26582)
                     /locus_tag="VFA54_06970"
     CDS             complement(25656..26582)
                     /locus_tag="VFA54_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HZS50582.1"
                     /translation="MRSLLTFLLGSGLLWAQLEGVIDIHAHVDPETIPRSIDAMELAR
                     LARVEGMRAVVFKNHYVPTAPIAFLVAREAPGIQVFGGIALNSAVGGINPAAVEQMVQ
                     LPGHFGRIVWMPTMDAESGGSANASPKTTRKGGTSKNMGPPRAYQHTVPVARNGELLP
                     AVREVLGIIARENLVLATGHLDPETSLLLVREARKLGITHIVITHPVNRMSIEQQKEA
                     ARMGAYLEYCYVGTLEFAGSGRHTLEDYAGFMRELGPEHCIMSTDLGNAANPVHTAGF
                     KSYIAGLKRAGISDKDIDLMARRNPARLLGLE"
     gene            26925..28124
                     /locus_tag="VFA54_06975"
     CDS             26925..28124
                     /locus_tag="VFA54_06975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZS50583.1"
                     /translation="MSRLESERLTSATRRSALRRLAAFLAGSPLLRSQQDPFRDHSRV
                     PAIHELITALDFEPVAYAKVPRDAYDYTAYGADSEFTLRRNRQAFDWVELVPKGVVNV
                     SSIKTATEVLGTKMNFPIMISPSATHQQLRPQGELATHQGAQAASNTPMIVSNVSSYP
                     IDKIAAAANSPLWFQLYPREEFDENLPVIEAARAAGCQAIVMTVDQTASYYERAMHDR
                     HLESTRRRGQRAPARRRSAPGNPYRVSEGRLWYNWQFVDKLRPLVKLPLLAKGILTAE
                     DAQLCIEHGLNGVYISNHGGRSMDYDPSTLEVLPEIVDAVRGRVPVLIDGGFRRGSDI
                     LKALALGASAVCLGRVPRWGLAAYGAPGVQRVLEMVQTELALAMAYTGRPSLDSIDRT
                     LVKTDFS"
     gene            28289..28798
                     /locus_tag="VFA54_06980"
     CDS             28289..28798
                     /locus_tag="VFA54_06980"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZS50584.1"
                     /translation="MGMLPEPWLRGPIPSVNPFIAPLLYCFQQAREDLAKYTEDLTEN
                     QIWATPHGFGSVGFHIRHIAGSTERLVTYLQGNQLSEEQLVEMQAEKQPGGTREQLLA
                     RLEAAFQHAESVARGLDPAILADPRAVGRSRLPTTVIGLMTHIAEHTQRHVGQAISAA
                     KLAKALGQP"
BASE COUNT         5561 a         9022 c         8571 g         5784 t
ORIGIN      
        1 gcgcttgtgt tccaccagcc gtttcgcctt ctcaatcacc atttcggcca cctgcacgtg
       61 ccgcgccgcc ggctcgtcga acgtcgagct gatgacctcg cccttcaccg accgctgcat
      121 gtcggtgact tcctcgggcc gctcatcgat cagcaggacg atcaaggtga tttccgggtg
      181 attcgtggtg atcgagttgg cgatggattg cagcagcatg gtcttgccag tgcgcggggg
      241 cgctacgatc aggccgcgct gccctttgcc gatgggagtg agcaggtcca tcacccgtcc
      301 ggagaggttg tccttcgtcg tttccagccg gattcgcccg ctggggtaga gcggcgtaag
      361 gttatcgaaa aagattttgt ttcggccttc ctctggaggc tcaaagttga tggcctcgac
      421 cttgataagg gcgaaatagc gctctccttc tttcggcggc cggacatggc cggaaacggt
      481 gtcgccggtg cgcaagtcga agcggcggat ttgggaagga gaaacataga tgtcgtccgg
      541 gccaggaaga tagttatatt cgggagcccg caaaaatccg aagccgtcgg ggagcgtttc
      601 gaggacgccc tcggagaaaa tctggccgct tttttcggcc tgcgcctgga ggattttgaa
      661 aataaggtcc tgcttgcgca tgccggccgc gccgaccacg cctaattctt tggcgacctg
      721 gttcagcttg gtgatattca tctccttgag cttcgccaga tccaattcgt tttcttgtgt
      781 acgggttgtt gccataaaca cctcaggtac aggggaatcc cgagtggatg gagtcaggaa
      841 ccccagatgt cagcagggca agacggaatt tctcccgtct cgcccgccgg gcgcatctgc
      901 ggtctcaaac gattctgcta atgcgtcacg ttctcttcag ccgcgggttg ggatgccaac
      961 tcgacggccg gcgccatggg gacggccaac tcaagcggcc gctccagagc aatttgcagc
     1021 acttggtcca tgttctcgac aaagtgcagc ttcatcgccg tgcggatatt ttccggaatg
     1081 tctggcaggt ctttctcgtt gtccttcggc agcacagcct caaagattcc ctgccggtgg
     1141 gcggccagga gtttttcctt caagccgccg attggcaata ctttgccgcg cagggtaatc
     1201 tcgccggtca tcgccacgtc gccgcggacc ggaatcttcg tcagggcgct caccagagtc
     1261 gtggcgatgg tgatgccggc tgacggcccg tccttgggaa tcgcaccctc gggcacgtgg
     1321 atatgcacat ccaggttgcg gtagaaatcg cgcggcaagc ccagttgatg cgaacgcgag
     1381 cggacatagc tcatagccgc ctgggcggac tcctgcataa catcgcccag tttgccagtg
     1441 agcatgagct tgcccttgcc ttccatgagc gtcgtctcga cgttgagaat ctgcccgccc
     1501 acttcggtcc acgccagccc gaccgccgtg ccgatttcgt tcttcttttc cgattgcaag
     1561 tcgcggaatt tcggtacgcc gagaaattcg ggcacgttct cggcgtcaat ggtaatggaa
     1621 atttcctgtt tggggtcctt taccgcgcgg cgggccacct tgcggcaaac attgccgatc
     1681 tcgcgctcca gattccgcac gcccgactcg cgcgtgtatt cctgaatgat ccttaccagg
     1741 ccctcgcggg tgaaagtgag ctgcttttcg gtgagacccg tctcttcgcg ctgccgcttc
     1801 accaggaagc gcgtggcaat ctccaccttc tcgtcttccg tgtagccgct cagccggatg
     1861 acctccatgc ggtcttgcaa agcaggcgga atcgtgtgga gcacattggc ggtgcagatg
     1921 aaaaacacct tgctcaaatc gtattccacg tccaggtagt gatccacgaa catgtaattc
     1981 tgttccggat ccaggacctc catcaaagcc gccgacggat cgccgcggaa gtcggtggac
     2041 attttatcca cttcatcgag catgaagacc ggattgcgca tgccggcctt cttcatcatc
     2101 tggatgatct gccccggcag cgcgccgata tacgtgcgcc ggtggccgcg gatctcggct
     2161 tcatcgcgaa ctccgcccag ggacagacgc acgaacttcc gtcccgtggc cttggcgatc
     2221 gacatgccca gcgaggtctt tcccacgccg ggaggaccga caaagcagag gatggaaccg
     2281 cgcgggtttt tgaccagccg gcggacagcc aggaactcca ggatccgctc cttgatcttt
     2341 tccagcccgt agtgctcgga ttcgagcact ttctccgcgt agctgagatc gcgaatctcc
     2401 ttcgacttct tcttccacgg cacggcgagg agccaatcca ggtagttccg cgatacggtg
     2461 gactcagccg acatgggcgg catattctcc agccgcttca gctcggccaa agccttctcc
     2521 tgggcgtcct tggtcatgcc agccgattca atcttcttcc ggagctcgtc aaattcgctc
     2581 ttctcgccgc gcccgagctc cttttgaatg gccttgattt tctcgttgag atagtactct
     2641 ttctgggcct tctccatctg gcgcttgacg cgcccctgga tggtgcggtc catgttgagt
     2701 ttctcgatct cgatatccag cacgtcggcc acgcgggtga gccggtcgat cggatcgaaa
     2761 atctccagca gatcctgctt ctcttcgatc gtcaattgca ggttcgcgcc gatcgtgtcg
     2821 gccagcttgc ccgggtcatc gacgcggacc gccgcgatca tggtttcata gttcaaattc
     2881 tggcttagct ttacatactg ctcgaacagc gacgtgacac ggccgatcag cgcctcgagc
     2941 tgctggccgt tctccacctt gtagctggtg gtcttgacga tcgcccggaa gaatccttca
     3001 tcttcgctca ccgagaccac tttcgcccgt tccactccct ccacgagcac cttgatgttc
     3061 ccatcgggga gttttaggct ttgcacgatg ttgacgatcg tgccgacgct gtaaatctcg
     3121 ttgggccgcg gctcatcgac cgaagcatcg tgctgcgtgg ccagaaagat cttcttatct
     3181 ccggccaggg cctcttccag cgcccgcacg ctcgattccc gccccacgac gaacggggtc
     3241 atcataaacg gaaaaatgac cacgtcgcgg atcggcatca tcggcagccg cttggtttcg
     3301 gatttttctc gggttgtttg cataagacct tcgcgcggga cgcgggctat ccggctttct
     3361 ccagcaaatt cagattcaga tcgtggttca gaaccatctc ccgggtcacc acaaactcgc
     3421 gcaccttttt gtacgagggt aaataataca tcaggtccaa catcatctct tccaggatca
     3481 tgcgcagccc gcgtgctccc actttgcgct cgagcgcaag atcggcgatc gcctctacgg
     3541 cgtcgtcctc aaaacgcagc cggacgttct caaactcgaa cagcttctga tactgccgca
     3601 cgatggcgtt gcgcggctta gtcagaatct ggaccagggc ctgctggtcg agatcgctca
     3661 gcaccgccac cacgggaagc cggccgacga actccggaat gaagccgaac ttgatcagat
     3721 cgtggggctg cacctgctct agcaggtcga tatccctggc gccatcggca gaggcgcgcc
     3781 ttgccttttc ttcctccgcc gcgtggggcg agcggaatcc cactgacttt ttacccaccc
     3841 gccggccaat cactttctcc agccccacga atgcgccgcc acagacgaac aggatgttgg
     3901 tcgtatcgac cggcgtgaac tcctggtgcg gatgtttgcg gccgccctgc gggggaacgt
     3961 tggcaatcgt accctccagg atcttcagca gcgcttgctg cactccctcg cccgagacgt
     4021 cccgcgtaat cgatggattc tcgtccttgc ggccgatctt gtcgatctcg tcaatataaa
     4081 tgatgccctg ctgcgtcttc tggacgtcgc cgtcagcggc ttgcagcagc ttcaggatga
     4141 tattttccac atcctccccc acgtagccgg cctccgtgag ggttgtagcg tccacaatcg
     4201 cgaaggggac gtccaaaatt ttggcgagcg tctgcgccag aagagtcttg ccggtgccgg
     4261 taggaccaat cagcaggata ttgctcttgg caagctccac gtcgcccatc ctctggcggt
     4321 tcatggagat gcgcttgtag tggttataga cggccacggc cagctttttt ttcgggccgt
     4381 cctgccccac gacatattga tccaaaagct ccttgatttc ttgcggccgc tggagcctcg
     4441 gcgctcccac cggaacccgc tcggccttat cgtcctccag aatggaattg caaaccgcga
     4501 cgcactcgtc acaaatatac gccttcggat agtcgctcgg ggtggagatc agcttgccga
     4561 ctacctcctg gctcttgtga cagaatgagc accgcaaaga atcatccggc ccgtttcttt
     4621 tcactactac gcccctcccg cctcacaaaa ccgcctcgcc cgcaggctac tcctcctgcc
     4681 tgtcttcatt atgcggccag cagccgggcc ttggcaatag atcatctcgc cggccgcatt
     4741 gcgcgcaata gggaggagca cctatttctc gttctggaat tcagtgctta acgatgattt
     4801 cgtctactaa cccgtattcc ttcgcctgcg ccgcattcat aatgaagtcg cgctccacgt
     4861 cgcgctggat cttctcgagc ggctggccgg tatgtttcgc cagaatctcg ttggtgatct
     4921 cccgcatgcg caggatctcc ctcgcctgga tatcgatatc ggtcgcctgc cccgacaaac
     4981 cgcccatcga cggctgatga atcagcatgc gcgagttggg cagcgcaaaa cgtttccctt
     5041 tcgttcccgc cgccagcagc aaagcggcca tggaagccgc ctggccgatg caataggtgg
     5101 tgatgtcggg gcggataaac tgcatggtgt cgtaaatggc catgcctgcg gtaatggatc
     5161 cgcccggcga gttgatgtac aacgagatgt ctttctccgg atcctccgcc tccaggtaca
     5221 gcagttgcgc agtgatcagg ttcgcgacgt tgtcatcgac cgggaacccc agaaagataa
     5281 tgttgtcgcg cagcaggcgc gagtaaatat cgtaggcgcg ctccccgcga ttggtctgct
     5341 cgacgaccat cgggataaga cccagatgag ggaccgttga catagacatc ctattgttga
     5401 aaccgatgac gccggacgcg cctcgcgttc ctaactcttt cttgcctgtt caaataaaaa
     5461 ctgcaacgtt ttttcgctgc gtatctgcga agcgatccgg tcgagcgccc cgctctccgc
     5521 cagcttggct cgggcaaccg ccaccggctg gcccgtctgc cgcgcataca gttgcacctg
     5581 gcgatccact tcttcttgcg tcgcctggat ccgctcctgc gcggcgatct tgtcaaggat
     5641 cagcccggcc ttgatttccc gaatggaccg cggctgctct acttcctgaa tcttcttcca
     5701 gtccagccgc agcttggtcg ggtcaacgcc gcggtccgcc agcgaccgca ggctgcgctc
     5761 cacattggaa tgaatctgct tttccaccaa tgcctcgggt acagggaagt cgtgggcgtc
     5821 caccaagtcc tcgatcagct tcgccttggc tttcctatgg gcctcttcgc gcttctgcgc
     5881 ttgcaattcg ctccggatcc gctcccgcag ctcggccaaa tcccggaaat cgccttcatc
     5941 tttggccagc tcgtcgttca gttcgggcag ctcctttttc cgcaagccgc gcacctccgc
     6001 gtggaacgcc accgtctgcc cggccagttt tggctcgccg taatcttccg ggtaagtcac
     6061 ctggatttcg cgctggtctc ccgggctcaa gccgcgcaaa ttctccgtga aaggagccag
     6121 cgtgtcctcg ccgccgattt cgacggtcaa ctcatcctgg tgaataggag ccatccccgc
     6181 cggagtctga gtggaccgga gactcaccac cgcgatatcg ccgtcggcca ggggcctcgg
     6241 atccaaattg cggtaaattg cgtgcctttc ccggatctgc tccagagcgg ccgcaatctc
     6301 ttcctcggta acctccggtt cgtcatatgc aaccggcagg cctcggtact ccttgagctc
     6361 aaaatccgga agtacttcaa actccgcctt aaaggtcagc ggctgccccg gctcgaattt
     6421 tacattgctg acgtccggcg tgcccaccgg atgcaacccc tccgcttcaa tttgcttata
     6481 cagatgccgc gggatgagcg cttccatgac ttcccgccgg atctcgtccg caaagcggag
     6541 gcgaaccatg gaggcggggg cctttccagg ccgaaagccg gggatgcgag cgcggtttct
     6601 gatttttgtg attactcgct cgttttcctg ttctactgct tcgaccggaa cagttatctc
     6661 gagctcatgc ttgcagccgg cgactaaggg caacgtttag gagcctcaac ctaactttta
     6721 gtaaatacct caatgtgcga gggctgtcaa tgaatgagat gcgctcagca caggatttgg
     6781 ctcatcggcc gaggatttcc atccattggc cccgcatttg ccacccggcg atgcggcgca
     6841 gatgtaagct ttcctccaga gaatcggacg taatctgtgg ggtgtagccg aagtatttgc
     6901 ggagcaattc ggccacgggc ggccagccct gcacgccgcc gggcggatcc caactgcgcg
     6961 agtatagcac gaaaatctga acggcctcag gactcacacg gcgcagggcc tccggctgaa
     7021 agttctcaac cgcattgact tttaacgggc gccggacgta acccccgagc ggattgacga
     7081 gcgcgtcggt cagtggccac gctgtagtga cggtctgatc gccggaatgt atggtaagcc
     7141 actgcgcggc gcgctgctgc aaacgtatga aatccaccac cgcaaggttg tcctcgtagc
     7201 tgaacgggta gggcgggttc caccaattgg agatcaccag gccggctgca agagtcccca
     7261 gaccagccct gcgcagcgcc gcaggcagca attcggaagc cgcggctacg gcgatataga
     7321 acagcgccac cgcaggcagc aagtatcgca ggaggacggc gcctcctacg gcgctgtgca
     7381 gcagcacgta gcccgcgatc actgtcagaa ccagcttcca gcgccggccg cgaagcgcgc
     7441 catatcgcag aatggccaag gccagcagtg ccgagccgat ccaatggaag tcgcttatgc
     7501 caagttgata acaacggcgc aggaatacca gcaggatgcg cagtccgtca aaagcccgag
     7561 ccaggttata agccgcaaat tcgctgttgc cgaaccaatg cccggttttt gaccgcagaa
     7621 acagcagcca gaccgcaatc gccccgaagg ggggcagcag cggaagaatc gggaagcttg
     7681 cgctgcacca ggcccgcacc ggcgccggcg caccagccat tccctttggc gttccggaat
     7741 tccaaacgcc cttttggttt tgccaaaacg cgtgcaggga aaatgcaatc agcgccatcg
     7801 ggacgagcaa accggtttct ttggtgagaa ccaaggcggt cgccgcagcc ccgcagcgcc
     7861 aaggccggcg ttcgagaaag aaatagagag acaaaagaca ccagagagcg gcagccaaat
     7921 ccaactgcgc cagcatgctc tgaatcacga acgggggcga taaggctgcc aggaacgcgg
     7981 cagcgcaggc cgcgcgcgtg gaaccggaca ggcggcgggc cagcgcgaag atagccgcca
     8041 gcgtggctgc cccgatgccc agcatcgcca cacgggtcgc tggaatctgg aagccaaaga
     8101 gcatccacac gcccgccaga tacagcatca gcaggggcgg atgtacgttg ggtaacgtgg
     8161 accgcgggat tagggagtgc cagtgaagca ggtcgtgggc cgcagggaca aaatagccca
     8221 actcgtccca atggtaggga aggtcaagaa atcggatgtg ggtaatcagc aggaaaagga
     8281 agccggcggc gaagcagacg attggcggtt tccggagatg cagccccgca ccccgcaaga
     8341 tactatgaaa acggccgcct ggccaatcac tgtacgacac ccatcgccgc cttggcgtcc
     8401 agcttgagtt ctccgaaagt attctcgtat tgggttacgt tctgctgtaa cacgttcagc
     8461 aaggctttcg cctgctgggg actcagataa atgccctgaa acgtctcgat gttcaccact
     8521 tcagggtcgg tctgcgacac cgttccaaat tccaaataga aatcccaaag attgacccgc
     8581 atctgcacgc tgttggcgta gttctcgcgg aagtcggccc gccgaacgac tttgatcttc
     8641 acggattgtg gttgggtcat gcgctctatg ctgccgcagc ttgattgcct gcgtcaacca
     8701 cggtccgcca gcgcgatcac agcgccttcc gcacagcgtc cagcacgccg ttaacaaact
     8761 gcacagactc ttctcccgag aagcggcggg cgatttccaa cgcttcgtcg atcactacgg
     8821 gcggaggggt ctgtcgctgg agcagctcga agatagccag ccggagaacg ttccggtcca
     8881 cggccgacat acgttccagg cgccagtgct cggcgtactg gcggatgagc gcgtcggctt
     8941 cggcgacccg cttcactgcg ccgcgcagca gctcgttggc gaattcatcg acgggcttgc
     9001 gccggggaga ctcctcgccg tgcatttcct cggaaaacaa gccaccccag tagccggcaa
     9061 tgacttgctc cgggctctcc cggctctggt cccactgaaa cagcatctgg agagcgcgct
     9121 cgcgcgattc gtggcgggag gccatgctac ttctttttgg cccgcacttg cttcatcaga
     9181 ttcgccattt caatggccgt caaggccgct tcgaatccct tgttcccgct tttcgccccg
     9241 gcccggtcaa tcgcctgctc gagcgtgtcg caggtaagga cgccgaagat gacgggaatt
     9301 ccggtttcca tcgcggcctg cgcgatgcct ttggacgctt cttgcgcgac atagtcgtaa
     9361 tgcgtggtct cgccgcggat caccgctccc aggcagatga ccgcgtcgta ttggccgctg
     9421 cggcccagtt cccgggcgat aagcggaagt tcgcgggatc ccgggacgcg caccacctcg
     9481 atggatttcg gatcacaccc ggagcgctcc aggccgtcca ccgccccggc gagcagccgc
     9541 tcggtaataa aactgttaaa acggctcacc gcaatggcaa acttcagtcc ggctgcttcc
     9601 agcttccctt ccacagtcac gatattctgg ttcatacttc catccactgg cgctgctatg
     9661 gcgtctctgc cagacggccc agagcatccg ctccgaacct cgcagttctt ctcctgcgtc
     9721 tactgcatcg cgaatcttac gctccgcgga accgggcttc tcgtttctcg aggaaagccc
     9781 gtgtgccttc gcgcatatct tcggtcccgc agcacaagga gaacgcggcg gcctccaaag
     9841 cgagggccga atcgagcggc atttcaagtc cggcgtttac ggcgttcaag cacatggata
     9901 cagccatcgg ggcggcggca agcattcggg ccgcccattg ccgcgcggcg gcgagcaact
     9961 ccccctgcgg cacaatttgc tgaacgaggc ctattcgata cgcttcgctc gccgaaatcg
    10021 ggtcgccggt tagcagcaca accagcgccg cggccttccc caccagccgc ggcagccgct
    10081 gcgtgccgcc gtagccgggc acaatcccga gtttcacttc cggctgcccc aaccgcgcgt
    10141 tttccgaagc aatgcgaatc gagcaagcca tcgccagctc cagccctccg cccagcgcaa
    10201 atccgttgat ggcggcaatc gtcggctttg gcagcgactc aatccggctg aacaaacgct
    10261 ggcccctcga cgagtattct ctggcctcca gcgggcgcag ttgcgccagt tcgtttatgt
    10321 cggcgcccgc cacaaaggcc ttctctcccg ctcccgtgaa gaccgcaacg cgaacggccg
    10381 ggtcgcgctc gatttcgctg acgcagcgct cgagatccaa aatcgtcccc cgattcagtg
    10441 cgttatattt ttccgggcgg tttaccgtaa ccagcgctat cgagtgttcc acttcataga
    10501 gcaggttttg gtattccata gccagacagc atatcagttg gggtgacaga ttgcaggcca
    10561 tagccgtttg ccattcgcgg ctgagcccgg aagcggccgg cggctatcga gcgcaggaac
    10621 gccacaggat ccccggggcg gcctacagtc ccggccttct tgtgataccg tgaaagcggt
    10681 gcctatcgac cggtcgctga ttcgtaattt ctctatcatc gcccacatcg accacggaaa
    10741 atcaacgctc gccgaccgcc ttctggaggc gaccggagcc ctcgagcaac gggaaatgat
    10801 ggagcaggtg ctcgattcca tggatctcga gcgcgagcgg ggaattacca tcaaggctca
    10861 cgccgtccgt ctgatgtaca aagccgacga cggccaaacg tatcagctca acctgattga
    10921 tacgcccggc cacgtggatt tctcgtatga agtctcgcgg agcctggcgg cttgcgaggg
    10981 cgccctgctg gtagtggacg cctcacaggg agtcgaggcc cagacgctcg cgaatacata
    11041 cctcgctctg catcacaacc tcgaaattat cccggtcatc aataagatcg atttgcccgc
    11101 cgccgagccc ggccgcatcc gcgagcagat cgaaaacatc atcggcctga gcgcgcagga
    11161 cgctctgctg gtgagcgcca agcaaggcgt cgggattcat gagacgctgg aggccgtcgt
    11221 gaagcgcgtt cccccgccct ccggttcgcc ggaggctccg ctccgcgcgc tgctgttcga
    11281 ttcctggttt gacgcctacc ggggcgtgat tcttctgatc cgcgtggtcg aaggcaggtt
    11341 gcgcctggga gcgcacatcc gcatgtggtc gagcgggcgg gtctacgaag tcgaaggctt
    11401 gggataccgg actccgaaaa gctactcctg cgaagagctt tccgccggcg aggtgggttt
    11461 cgtagttgcc aatatcaagc acgtggcgga gacgcgcatc ggcgatacca tcctggatga
    11521 tgacaacccg gcggaagagc cgctgccagg attcgaggag agcaagccga tggtcttcgc
    11581 cggcctgtat ccggtggaag cccatgcgca tggagagctg cgcgaagccc tggaaaagct
    11641 tcaattaaac gatagctcgc tgcgctttga gccggagagc tccggcgcgc tgggattcgg
    11701 ttttcgctgc ggctttctgg ggctgttgca tcttgaaatc gtccaggagc ggctggagcg
    11761 ggaattcggc ctcaacctga tcactacggc gccgagcgtc agctaccggg tcacgacgcg
    11821 atcgggcgat gtcatcgagg ttgacaaccc gatgaagttc ccgccgccgg ccgagatcgc
    11881 gacgattgaa gagccggtgc tgtcggtcac catcatcacg cgggacgagt acctgggcgg
    11941 catcctcaag ctgctcgagg agaagcgcgg cgtgcaaaaa ggattcgagt atgtgggcgc
    12001 caaccgggtg ctgctcacct acgaagtgcc gctcaacgaa gtggtcctcg atttttacga
    12061 ccggctcaaa agcgtgtcgc gcggctacgc ctcgctcgat tatcacttcg ccggatattg
    12121 gaattccccg ctggtaaagc tcgacattat ggtcgctggc gagactgtcg acgccttgtc
    12181 cttgatcgtt caccgggatc aggcatatga gcgcggcaaa gcgctcatcg agaaaatgcg
    12241 caagctgatt ccccggcaga tgtttgaagt cgcgctgcaa gccgctattg ggaacaaaat
    12301 catcgctcgg gaaaacatcg ccgcgctgcg caagaacgtg ctcgccaaat gctacggcgg
    12361 cgacatcacc cgcaagcgca aactgctgga aaaacaaaaa gaaggcaaac gccgcatgaa
    12421 gcgcgtcggc cgggtggata ttccccagga ggcgttcctg gcggtgctca aggtgggaga
    12481 ggaaagcgac tgagaaccgg ggcgtccggt ttggctgggg atctcaaaac cgggttgctg
    12541 gcagggtgag ccgatcgcgg ctaccctatt tccggtgcgt gacttccgcc gcccggcgtt
    12601 cccgctctac cgtggcgcgg gcaatcgccg ccagctccgc atcgacgagg aatcgcatca
    12661 atgatccgcc cacctcagcg cctcgctcca cgcgaagctc ctgaatgcgc tcaaacattt
    12721 tccagcgcat caagctgtgc ggacaaatgc cgtatcgaaa ttccattgaa gcctcctcac
    12781 ggacacggcc agaatatgcc gtctggctcg gcgcgaacaa gatgccttag gtaccggcct
    12841 ggtaaggcca agcccttaga aaagtctggt ccctgtatcc acacccgccg gcgggcgcct
    12901 gtatcccgcc aaaggcgccg cttgcgcgtc gattgtatgc caaaaacatt ctttacacaa
    12961 atgatttata gacgggcgga gcgcgccggg aagccgcctt tcggcccggg acgcggaatc
    13021 tgcgccagcc tcccacggca tctcttcttc catgggtttc cgacccgcat tcgattgtcg
    13081 ccctgccgga cggatgtggt tatggatgtg ttctattcgg gctgcacccg cctggtcgct
    13141 gtgaagcgag ggaatcatat cgaaatatcc ggcaccttcc agctttagta cggccacgga
    13201 gtaccgcctg gccgttgccg cggcttcata gccgctcacc agatcgtaca tggctagcag
    13261 aaatccgcct ctctatctat aacccgcgcg ggagcggcaa gctatcggcg aattgctgga
    13321 aaaacgccgg gagagcgtcc ctactcgaag ttcagggaat gctcagggct gtggaggaat
    13381 cgctgccgcc caagcgatca ttaagttcgt actggtttgc ttcttatgcc ggcaaagacc
    13441 tgccgccaac actggcgttt caggcgcgtg ccgcagcgag gcgatatttt tcccgcgcgg
    13501 gcgggtccgg cggccgcggc aggcttgctc ttttacggcg ctgggtaggg gttggacttc
    13561 cgaaaaagtc tttgccgcac gtcgggccag tactctttcg caatgttaaa caacgaatca
    13621 agcacggtct gcgatatggc cttgtaggcg gtttgatgca gggtgcgatc cggagcggga
    13681 tagtacacgt tggaaatagc ggcttgcgct ccattgccca gaaactcgga aaagttgaac
    13741 tgctgcgagc cggcgtcggt gcgcgtgacc agcacgcggc tcatcgcgta tccggcgcgc
    13801 gcaaaaaagt taccgcgccc ctttcgaaag taccggggat cctcgtgcag cacggaagga
    13861 aacaccgctt ctcccagcat attgccgatt gcttgatcgg cataggatcc cgcataccgc
    13921 ttgccgaaac cttccattcc ctgtccgaag gacttgtatt gattctgtag ctgctcgatg
    13981 cctgcgtata tcgcaatcac ggggagcgcg tgaggatcga aagaatcctt ggcgccgagc
    14041 ttaaatttct cccggatcgg catgggctcg accggctggg atgggtcttc cacggtctta
    14101 tagttcggaa tcaccttcag aatgtgctgg tcggccgggg gcttttcccc caccaccgca
    14161 gccggctcca tgccggcccg gtcggcgggc agatcgtcct ctacggctcg cgcaggcgcc
    14221 ggagcgatca cgcaggccag tatcagccca accagagggg tgcggaatcc ccagaaaaca
    14281 aacgatcccc gctgctggaa aattcggcat ggagacatta tgtaggccaa gacgcggggc
    14341 gccggctggt agttactcat taagacctgg ccgagcaagt ttctacttgc atttccacag
    14401 ctcccttttc gatgcattgc cctctcccgg cagccgtttc ggctagggat tgtctgcggc
    14461 ggaaatcgcg gcgccccgcc acacaagctt ccatcgcccc gccaaagccc ttcctggcag
    14521 tgccttaggc taccatgcct agagaaaacg gagaaaaact gtggcctacc actgggatga
    14581 gttgacgccg tttgaggctt tgcccgatcg tggggaaggg gtcaaggttg cggttcccga
    14641 gaagattttg aaactgtggg gcaacttcca gatgccgaag ccgccatcgg aagcgcgcgc
    14701 ggccgtctcc tttaacttcg tgcagacgct ggacggcgta gcaagctatg gagtgcgggg
    14761 attcgccgga ggtggcgcta tttccaagaa gcgcgaagac cggtggatga tggacgctta
    14821 ccgcacgatg accgatgcca ttgtgttggg tgctgaaacc gttcgcgccg aggatcggga
    14881 aatcaaaagg cggcgggagg ccggcggcag accgctttat gaaatcgacg ggatgccgac
    14941 caacggcttt gtcgccacgg tggaagatcc ggagcttgcg gaatggcggc gccggactcg
    15001 cggtgagaga catccggtcc tggttgtgat taccgctagc ggagaactgc ccgaggacct
    15061 cggcattttc gacctcgctt cccaacggat cctcatcttc accacccccg ccggagagtc
    15121 cgtccttata gagatgatgc gaaacaagcg gcgatttgcc aacgtggaaa caatcccgtt
    15181 cggaggtatt ccagcggatc tgccgctgtt tctgaccgga atcctcaagc agaaattcgg
    15241 aatccgctac gcactctgcg aaggcggccc cgccacggcg ggcgcctttc tccaggacgg
    15301 cctggatgac gaattgcttc tgacagtcgc gccgctgctc gccaccggca aagacgaggg
    15361 cggccaggca agaaagcgca tcgtagaggg acccgatttt tatccggccg cggcgttcga
    15421 tgcccctggt ttgcgcatcc cgcccggggc gcggcgctcc ccagagtatc tgcttcacag
    15481 cgtccgccgc cacggcgacc acatgttttt tcgctactgg cggcagcggt cttagcccgc
    15541 acttccaaaa aaagcggctg ccagcgcccg tttggctcat cttttcaccg gcgctgcggc
    15601 ggccgtggcg cttgggaagc gagaatggcg ccgttgggga acaaattccg gacgataatg
    15661 aaaattggag gcgttctatg gtggtcggcg taccgaaaga gactttcccg ggggagcggc
    15721 gcgtggccct ggttcccgga gtggttccca cgctgaccaa ggccggattc gaagtgctga
    15781 tcgaggccgg tgcgggagtg caggcggggt attccgatgc ggagtacgag gggaaagggg
    15841 cgaaaatcgt ttcgacacgg gctgaagttt ttgccgccga catcgtggcg caggtgcttt
    15901 gttacggctc gaatgacaag actggcaagg ccgatctgcc cttgcttcgc cgcaatcagg
    15961 tcctcgtggg ctttctccgg cccttgggat cgatcgatac gattcaggaa atcgcggaca
    16021 cgggagtcac ggccttcgcc gtcgaactag tgccgaggat cactcgcgcg caaagcatgg
    16081 acgccctctc ctcgatggga acgatctgcg gttataaggc ggtgttgctg gcggccgaca
    16141 cgctgccccg catctttccc atgctgacga cggcggccgg aacgatcacg ccggcgcggg
    16201 tgttcgtaat cggggccggc gtcgcaggcc tgcaagccat tgcgacggcc cgccggctgg
    16261 gggcaatcgc cttcgcttat gacctgcgcc ccgcggccaa agagcaggtg cagagcctgg
    16321 gcgggcgttt cgtcgagctg cccatcgagg ccaaggatgc acaggacgcg aggggctacg
    16381 gccgtgctca ggacgaatcg ttttatcagc gccagcggga gctgctgggc cgggtggtct
    16441 cggaaagcga tgtcgtaatc acggccgcgg tcgtccccgg caaaaaatcc ccggttcttg
    16501 tcacaaaaga gatggttgcg tgcatggcgc caggctctgt gatcgtggat ttggcggcgg
    16561 agcgcggcgg caactgcgag ctgacgcgca ccggcgaaat cgctgtggag cacggcgtga
    16621 cgatcatcgg ctatatcaat ctcgctagca tggtgcctta ccacgccagc cagatgtacg
    16681 cgcgcaacat tgccgctttt ctgttgcacc tatgcaaggg cggccagctt caaatcgatg
    16741 tgaccgacga aattacacgc gagacccttc tcacccggga cggagaagtg gtcaacgccc
    16801 tggtgcggca gtttttctcg ctgccggtcc ccgctgccgc aaccagtcgc tgagatatag
    16861 cggatcttcc gccccaagga ggaacattgc cgtccgatct gattaccagc ctttacgtgt
    16921 ttgttctggc gggcttcgtc ggattccagg tgatccggcg agtatcgccg cttctgcata
    16981 cgcccttgat gtcgctgacc aacgccctgg acgccatcgc cgtggtcgga gcgattgtaa
    17041 tcgcagggga acgcaagagc acgctctcca cggtgctcgg aacccttgcc gttgtggcgg
    17101 cgacgagcaa cgtggtgggc ggcttcctga tcacagaccg gatgctccgg atgttcaaag
    17161 aaagcagacc caggaaataa ctcgcggtca tgagcccttt tctcaccgat caggtcatcg
    17221 agattgtcta cctcgtcacc accgttctct ttatcttctc gttgcaatgg atgagcgcgc
    17281 cctcctcagc ccggcgcggc gtgctggccg gggagatcgg aatgctgctg gccatcggcg
    17341 gaacgctgct gcaccgcggc atcattgact accggcccgt cgtgatcgcg ctggttgctg
    17401 gttcgatcat cggcgttcct ctcggactgg tgcacatgac ggccgtgccc cagcgaaccg
    17461 cgctgagcca cgccttcggg gccttcgctg tggctctggt cggctcggcc gaatattatc
    17521 tgcgcgcccc cggagtctcc ccgttcatga tgtcagtgct ggcagcggaa gtgatcctcg
    17581 gctcgcttac cttcaccgga agcctgatgg cggccgggaa gctccaggaa attctgcccc
    17641 agcggcccat tacctacaag gggcagaacg tcgtgaacct ggggctgctc ggcatcgcag
    17701 tcgcgatcgc tgtcgagttg gtgacgcacc cggagcgaaa aattcttttc ccgctcatca
    17761 tcgcgcttcc tctgctgttc ggggtgctga tgattgtccc gatcggaggc gctgatatgc
    17821 ccaccgtcat atccctgctg aattcctacg cgggtctttc cgccgccgcc atgggatttg
    17881 tgctcgacag caagctcctg atcgtcgccg gcgctctgga cggctcgtcg ggcttcattc
    17941 tgtcggtgat catgtcgaaa gcaatgaatc gatcgttcac caacgttctg ttcggcgcat
    18001 tcgggcaggt ccagccttcg gcggcggcag cgaagcagga gcgccccgtg cgcagcgcaa
    18061 ccgccgagga agccgccgcg atccttgcgg cggccgatac cgtggtgatt gttcccggct
    18121 acggcatggc cgtggcgcag gcacagcaca aggtccgcga gctttatgac gctctcacca
    18181 aacgaggcgt cgatgtgaaa ttcggtattc acccagtcgc cggacgcatg ccgggccaca
    18241 tgaatgtatt gctggcggaa gcagatattc cctatgacaa gctgctcgac atggatgcgg
    18301 ttaatccgga ttttccgcag actgatgtcg ctttgatcat cggcgccaac gacgtcacca
    18361 accccgcggc ccgcaacgac ccgggcagtc cgatctacgg catgccgatc ttggatgtgg
    18421 ataaagcgcg gaccgtgatg gtgatcaagc gcagcctgag ccccggcttt gcaggcgtcg
    18481 ataatcccct ctattactta gataaaacgc taatgctttt cggcgacgcc aaagcctttg
    18541 taggggatat tgttcgggaa ttggggggcg agcatcggta agctccccgg aggctctccc
    18601 ggagttccag ctcctgtgcc ggttgctcca ctcctccaag agcaggcgtc ctttgtccac
    18661 tcgggatgat acgtgcctgc ccgtacaaaa ttttatcctt gagagtctgc ggccgctgga
    18721 tttcagcgcc tagacccgcc gctgatcgca gctacaaaat ggacctcgct cgtcggcgac
    18781 acccgctcga gcagccccag cgggctgact tcgccatcga ccgcgacaga aatgttggga
    18841 cgaaggtggt ctttcgctac caggcgctcc cgaatgcccg gccatgactg ttccaggttg
    18901 tcgatgatct ggcgcacagt cgcgccttcg acctctaccg ttgcatttcc accggtcaga
    18961 gcgaccagcg gcgtgggaat gtagacaatc gccacgtcaa gccttcgcgt tccaaatagc
    19021 ttccatcacg accgacggct tcatgggaag actgttcagc cggacgccgg ctgcacggta
    19081 aatcgcattt gccagcgccg cgggcggcgg gacgatactg gcttcaccga cgccgcgcgc
    19141 cccaaacgga tggcctggat tgggcacctc cacgatcacc gtttcgatcg gcggcagatc
    19201 gaggctggtc ggcatacggt aatcgagcaa actggcgttc agcatcactc cctcagggct
    19261 catgtagtac tcctcattca gcgcccagcc tattccctga acgctgcctc cctgcatctg
    19321 cccctccaca tagctcggat gaatggcctt cccggcgtcc tggatcgatg taaaccgtag
    19381 aaccgaaacc ttacctgttt ccgggtccac ttccacatcg gcgatgcagc cggcgaacga
    19441 gccgccgacc cctttggggt tgaccgctcc gcggccgatc accggcccgc cagtctcttc
    19501 cagatgcgcg gcgagctctt tgaaggagat actgcggtct ccggcgcaaa aaatgcctct
    19561 ttccgcccgc acctgctcca cgccgatctc ccacagcttc gccgcgcgct ctttcatttg
    19621 ccgcacaacg tcctgcgcgg cgtcgtaagc agcgagaccg gtagagaagg tgacccggct
    19681 gcccccggtc accgccgtat agccgaccga gtcggtatcg gccacagtag gccgaacgtc
    19741 ttcggccgca atgccaagca cctccgcggc ctgcatcgcg acagaagtcc gcgtgccgcc
    19801 gatgtcggta gatccttcga ccaggctcac cgtcccgtcc gcattcacgc tgatcgtgca
    19861 gctcgaagga agacccacgt taaaccagaa gccgatagcc acgccgcggc cgcgatgcgg
    19921 gccgcccagc ggcgcggtgt agtgcgggtg gcgcttcatg gcttcgagca cttccgcgca
    19981 cccgatgcgg cggtgcctgg ggccatcggc gcgccgcgtg ccttctttcg cggcattccg
    20041 caaccggaaa tcaatgggat cgatcccgag cttttcggcg atttcatcca ccacagtttc
    20101 cgcagcaaaa gcagcgttgg ttgcgccggg agctcggtag gcggccgtgc tcggcttgtt
    20161 taccacgaca tcaaagccgt cgattacgac gttctcgata tcgtaggcgg cgaagataca
    20221 catcgccccg ggagccacca tggcgccggg atacgctccc gcttcgtaag caaggtaagc
    20281 ctgcgccgcg gtgatgcgtc cgtccttgcg cgccccgatc ttcaccttga tgtaggagcc
    20341 cggagtgggg ccggtgcctt caaacacgtc cttccgcgac attacaatct tcaccgggcg
    20401 tccggtcttc ttggaaagca atgccgcaac gggctcgagg tacactggga tcttcccgcc
    20461 aaagccgccg ccgatttcca tcggcgtcac tttcacctgc gagacgggga tgtcgagaat
    20521 acatgccgtc gtgtcgcgca ccacaaaagc gccctgtgtg ctgcaccaga tgtggacccg
    20581 cccgtcgtga ttccaaagcg ctgtcgcgtt gtggggttcg atgtagccct gatgcacggt
    20641 ggccgtgcgg aactcgcgct cgacgatcac atcggcctcg cggaagccct tctcgatgtc
    20701 gcccaggctg tggcggaagt gctgggcgac attgctggcc tttgctgccc gtttcccaaa
    20761 ctcttctgtc cggaggtcgt cgtggagaat cggggcgccg gcctgcatcg cttccggagc
    20821 ggtgagcacg cagggcagcg gttcgtattc cacctcaatc atacccgccg cttcttccgc
    20881 aacatgaggg ctcacggcgg ccacggccgc gacggcgtgg cccttgtaga gcactttttc
    20941 tccggccagg atgtttccac gaatatagct gagcggcgtg gcgccttccc ccaggtcaac
    21001 aatgcggtcg ccggcgccgg gcaggtccgc cgccgtcacc gcggctttca ctccaggcag
    21061 cgcggctgct ttggccgtgt caatacggcg gatgcgggcg tgagcgtagg ggctgcgaac
    21121 gatgcagcca tgcagcagcc caggcaggcg aaggtcggcg ccatattgcg cgcggccggt
    21181 aactttatcg gcgccgtcgt gacgaatggg acgcgtgccg atgaccgcat actcccggcc
    21241 gccgatccca gtttccgtcg aagtctcggt ctgtgttttc atgctttcac cttttgcagt
    21301 tgcgccgctg cttgctcgac cgcccgcacg attttatcgt agccggtgca gcggcacagg
    21361 ttgttggcca gccaaagccg gatgcgctcc ggggatgggt ccggctcctc atcgagaagg
    21421 gccttcgaag ccacaatgaa tccgggagtg cagaacccgc attgcagggc ggcctgctcg
    21481 agaaacgccc gctgaaccgg atgcagctcc gcgccgcggg cgatgccctc gatcgtcgtg
    21541 atctcggacc ctgcggcttc caagccgagc accaggcagg aatttacgat gcggccgtcg
    21601 agaatgaccg tgcaagcccc gcagttgccg tcattgcatc cctccttggc cccggtcagt
    21661 ccgagctcgt cgcgcaggca ctcgagcaga ctctgccgcg gttcgcagag aaactcgacc
    21721 gtctcgccgt tgattgtcgc ttgaacttga gttctggctc gcatccgttt tatgcctccc
    21781 tggcccgctg gatagccgct tcgagcgccc gccgcgtgag cacggcgcat aaatgccggc
    21841 gatattctgc cgttccgcgc atatcggtga tcggcttcgc cgccgccttg gcgatctccg
    21901 cggcgcgatg gatgttctcc gcgttcaccg gcttgcctga gagccattcg cccgcttccg
    21961 gcacgtagag cggcacgggc gccaccgcag ccagagcgat gcgggccgct cgaaaacgcc
    22021 cgtcctccaa aacgaccgaa gccccggcgc ccgccacggc gatatccatc tcgttccggg
    22081 ggatgaatcg caaataacgc gctccggaat gcggctccgg gggcggcaga tgcagcgaaa
    22141 tcagcagttc gcccggctgg agcacagtgc ggcccgggcc ggtgcaaaac ttttccacct
    22201 cgatttcgcg gctgccggcc gcgccggcaa tatggcaggt ggtccgcaga gcaatcagga
    22261 cgggcactgc atccgcgctc ggcgcagcat tgcacagatt gccgccgata gaagcccgtc
    22321 cttgaatctg aatgccaccg atcaacgaag ctacttcgac caaggccggg tagaagcgtg
    22381 aggcggtccg atcttggtaa atccggcaac aagggacgca agcgcccagc gtgagcccgc
    22441 cggcgggatc gtaccggatc tcgttgagtt ccggaatgcg cttgacgtca atcacaagat
    22501 ccgccgcctt cctcccggac cgcaattgca ctagcagatc cgtgcctccg gcgaggattt
    22561 gtgctctggg attgctgccg agcagccgga cggcttccgc cactgagcga ggagcggcgt
    22621 aatcgaaggg tttcacgtcg aagtctcctt aaggtcctgc atccggggtg aaccttatag
    22681 ggtacaacga agcggaaacg ggctagaggg gcgctcttcg atcccgaaga acgaaaccgc
    22741 gctggagtgg cgcaaatgtc aaaggcgccc gactgcggag cctaagggca gcagaaagcc
    22801 agccggcttg gtcagcggcc gagttctcga atggccgcag gcggcggggc agccgccgct
    22861 cggcatgcaa gaccggccgc gaccgtgaat cagttgagct ttcctagcat ctcccgctgc
    22921 cgcgaaaggc gctgctcccg ctcctgcgcg aaacgctgga tgcccgagaa aatggactcg
    22981 gcgtcgaggt gagcctccgc aatcacgtcc ggctcgagac cgccggtgag ccagcggttg
    23041 tgccagtcgg aggtcaacga gtattcatcg gtcagcgggc cgacattgcg gattggccac
    23101 atgcggcggg tgccggtgct gaccaccatg agatcgtaat gcgcttcggg aggcagcact
    23161 gaattccgat acgcttccgg ctgccggtcg aagagatctt cgctgatggc ggcgatcact
    23221 tttacgttga tacccgctgc ctcaagccgg ggcagcgtgc tcaccagatt caccgttgaa
    23281 ctggagccct gggaaatcac ataaccgtgg cgcggcacgc ctggagtgaa gtcgcgaatg
    23341 acgtaaagac ctttggccgc ggcgcggaga tcgggatcgg caaatttgct ccgatcggcc
    23401 accgggaaat cgggccgcgc cacctcgatg gtgataatgc ccaccttggg atcgcgggcc
    23461 gcaatctcgg cagcggcgaa atagcccgga gccacgtcat tgtaatccca gaaattcaga
    23521 ttgatgacct gcccgcgcgg gaaaagcttc cacacctggg gagaaaagat gccgaagtgg
    23581 gtgcgcgcat cggcagcggt ttccggaccg gagtggccgg ccagaatatg cagaacgccc
    23641 atacgaaaac ggctgtcctg gttctgttgg ctccagacgc gggccggcag gtacatgagc
    23701 ggtgtaaagg cgccgtaagt gccgctgatc gcccacactc ccgcgaactt cgtggggtcc
    23761 acagaggcgc tctggccgac taagccgatg gcggttgaca cgttcccggc ctcttgaatg
    23821 gcggctttca ggcgcgtgcc gagcgggttt ttctcgggat cataatgtcc ccatagattg
    23881 ccgtgctcga gattgatgga ttcagagagg tcggcggcga tggtcaaaaa gcggttatcc
    23941 gtgacgtagt tgacccactt gatgatctct gaaatggccc gccgtgtccc cgcaacctcg
    24001 ccgggcttgc ggaacagcgc aatgctgact tgcttttcgg cgccggagta gcggtttttc
    24061 accgtgacct tctgcggctc gaccggcaga ttggcgaccc gcaaacgatc gtcaagaaag
    24121 gggtcccggt gaacatcgat gtgcagcggc aggttgtcct ttacagtgtc gccggtcgca
    24181 acgaggcgct cggccagcca atcgcccaaa ccgttgcggc cgagaaccga catgaccacg
    24241 tcgatattgg tcttgaactg aatcaggcgc tcgcgcggat cgctgacggg tcctttgcgg
    24301 atgccttcga actcgacgcc gtaatgcttt tcgaaggttt gggccagggc tacaaagtac
    24361 tccgagttca tcttcatggc gtaaggatgg ctcgggtagc tgacgatctg atcgatgttg
    24421 tccagaattt tgccgtccac ggcggcaggc cagtatccct tggtcgtgtt ggcgatcacg
    24481 atcatggggc gccgatcgcg tggatcccag tcttccatga tcttcagagc gctgacgatc
    24541 tggtcgtaat tgtggccgtt ttccagggag aaaacgttcc agccgtaaga ggtccactga
    24601 tcgatgatgc gatagccgtc gaatttttct gcgatgacgc cgccgataag cttgtcgtca
    24661 ataccggcgt tattgaagga aaacaggatg cgcaggcgct tgccgacctg caacgccaaa
    24721 gcctgggtct tcagttcctg cgcgtgaccc tcggtcatgg cgaactcacc ttcgaaagca
    24781 atcactttgg gggatccagc cggggcgccg atgaaatccc aaaaagcggc cttccccgcc
    24841 gccgtcgcga tgccgacgcc ggagggtccc ccgttgacat cattggtgac atccgtggtt
    24901 tccgcgtggc cagcaagagc gcggatgccg cgccccttgg cttgagcaaa cagcgggtgg
    24961 tcggccaagc cgtgattctg caacaggttc ttgagagccc ccgcgccgcg gcggaacccc
    25021 aaggcatcca ccggcaaaac ggcgacgctg ggatcgacat agtagcgccg gtcccgggtg
    25081 gctgcatatt tgcgcgccaa cgcctcgccc atgatcatcc acagggcgta gcaggtgggg
    25141 atgcagtgcc cgccggcgag catgaattta tcgctgtagg ggtgcttggg acggcggtaa
    25201 tcgtagcgca gcccgcccaa ttccggcggc gccaggtgaa ccgccacatt aaacggcgtg
    25261 tacgccaagg ggccgccaaa atgacccgtc tgagcgtagt tgcccaggag caccagggtc
    25321 atacaggtca tgtatccgat atcttccaaa cgctcccggt cgtattgcag cttcagcgct
    25381 tcgacgggcg actcaagtgt aggtgtagcc aatttcgaac cctccgcttt atgggccttc
    25441 ccagccccgc aaactatgaa gtcgatcctg ttcatgataa aacagatggc tcaaacgccg
    25501 cactatgggc gcccgcgccg aaaacccctt ccacgcgagc ccgcagacga tagacaccgg
    25561 ccggccttcc cggaaaagac atctccaccg gcccgcactc ccgcagccgc gttgatcgcc
    25621 tgatcggccg gaagcgctac aacggtcggg cagcgctatt ccaggcccaa cagccgggcc
    25681 ggatttcggc gggccatgag atcgatgtcc ttgtcgctga tgccggctct tttcagtccg
    25741 gcgatgtagc tcttgaatcc agccgtgtga accgggttag cagcgttgcc cagatcggtg
    25801 gacatgatgc aatgctcggg gcccagttcc ctcataaacc cagcatagtc ttccagcgtg
    25861 tgccggccag acccggcgaa ctccagtgtg cccacgtagc agtattcgag ataagccccc
    25921 atccgggccg cttccttctg ctgctcgatg ctcatccgat tgacaggatg ggtaattaca
    25981 atatgagtaa tacccaactt gcgcgcttca cggacgagca acaggcttgt ttccgggtcc
    26041 aggtgcccgg tcgcgaggac gagattttcc cgggcgataa ttcccagcac ctcgcgcact
    26101 gccggcagca gttcgccgtt gcgggccacg gggacggtgt gctggtaggc tctgggcggc
    26161 cccatatttt tgctggttcc cccctttcgc gtggtttttg ggctcgcgtt ggcgcttcct
    26221 ccgctttcag cgtccatggt gggcatccaa acaatccggc cgaaatgccc cggaagctgg
    26281 accatctgct cgacagcggc cgggttaatc ccacccaccg cactgttcag cgcgatgccg
    26341 ccgaaaacct gtatgcctgg cgcctcgcga gccaccagga aggcaatggg agcagtgggc
    26401 acataatggt tcttgaacac aacggctcgc atgccttcca cccgggccaa acgcgccaat
    26461 tccatggcgt cgatcgaacg aggaatggtt tccggatcca cgtgggcgtg aatatcgata
    26521 actccttcca gttgagccca caaaagaccc gagccgagaa ggaaggtgag aagcgacctc
    26581 atgcggttcc ctccgagtgc tccatcatag ccgaaggcag cgcggaggaa gcatcttgcg
    26641 ccaaggaatg cggcggcccg ccagggtagc gcggacacga tgaccgaacg cgtctccgcg
    26701 ggaaagataa ggatcgggaa agataaagat atgatagtct ggcgagttgt gcgccacgcc
    26761 gggaggcacg cgccagcttg atctggcgcg ccagagggct gaaattgtga gcctcatggc
    26821 ggcgtgggcg cgaaagcggc gcgcggcgga aaacaaggct ggaaaaaagg cacgcccccg
    26881 ataagcctcc ttcccgaatt cacagcgccg ccaggggaga tcgtatgagc cgattggaat
    26941 cggagcggct tacaagcgca acacgccgca gtgctctgcg gcgcttggcg gcttttctgg
    27001 ccggatcgcc cctgctccgc agtcagcagg acccgtttcg cgatcacagc cgcgtgccgg
    27061 cgatccacga actgatcacg gcgttggatt tcgaaccggt tgcttacgcg aaagtcccca
    27121 gagacgccta cgattacaca gcatacggcg cggatagcga gttcacgctg cgccgcaacc
    27181 ggcaggcgtt cgattgggtc gaactggttc cgaaaggcgt ggtgaacgtc agttctatca
    27241 agacggccac cgaggtgctc ggcacaaaga tgaactttcc gatcatgatc tcgccgagcg
    27301 ccacccatca gcaactgcgc ccgcaaggcg aactggctac acatcagggc gctcaggcgg
    27361 cgtccaatac gcccatgatt gtgagcaatg tctccagcta ccccattgat aagatcgcgg
    27421 cggccgccaa cagtcccttg tggttccagc tctaccctcg ggaagagttt gacgaaaacc
    27481 tgccggtgat cgaggcggcc cgggccgcgg gctgccaggc catcgtgatg acagtggatc
    27541 aaacggcctc ctactacgaa cgggcgatgc acgaccggca tctggagagc acgcggcgcc
    27601 gcggccagcg agccccagcc aggcgccgct ccgctcccgg caatccgtac agagtctccg
    27661 agggcaggct ttggtacaac tggcagttcg tcgataagct ccggcctctg gtgaaactcc
    27721 ctctattggc taaaggaatc ctgacggcgg aagacgccca gctctgcatc gagcacggtc
    27781 tgaatggcgt gtatatttcc aaccacggcg gccgctcgat ggattatgac ccctccacgc
    27841 tcgaggtatt gccggaaatc gtggacgcgg ttcgagggcg cgtgccggtc ctcatcgacg
    27901 gcggctttcg gcgcgggtcg gacatcctca aggccctggc gctcggagca agcgccgttt
    27961 gcctcggcag agtgccgcgc tgggggctcg cagcttacgg cgcgcccgga gtacagcgcg
    28021 tgctcgaaat ggtccaaaca gagttggcgc tcgccatggc gtataccgga cgcccaagcc
    28081 tggattccat tgaccgtacc ttggtaaaga cagacttttc ctaggcggac tacctccttg
    28141 cggcgcaggg gcctggctga aagacatccc cggacaggag cattaacccg gcggcatcgc
    28201 gggcgtggcc tcacggctcc gccttgacgc acgacattgc aatacaatcg aatcagaagg
    28261 actttgcggc gcgagccgct tccgcattat gggaatgctt ccggagccct ggctgcgcgg
    28321 tccgatcccc tcggtcaacc catttatcgc ccctcttctt tactgctttc aacaagcgcg
    28381 agaagacctc gcgaaataca cggaagacct cacggaaaat caaatttggg ccacgccgca
    28441 tggcttcgga tccgttggat ttcacattcg gcacatcgcc ggcagcaccg aacgcctggt
    28501 cacttatctc caaggcaatc agctttccga ggagcagtta gttgagatgc aggcggaaaa
    28561 acagcccgga gggacgcgcg aacaattgct ggcgcgctta gaagcggcat ttcaacatgc
    28621 cgaatcagta gcgcgcgggc ttgatccggc cattctggcc gacccccgcg cggtgggccg
    28681 cagccggctg cccacgaccg tcatcggcct gatgactcat atcgcagaac atacccagag
    28741 gcatgtaggg caggcgatca gcgctgcaaa actggcgaaa gcgctgggcc agccataacg
    28801 gcggagcgcg ccgctccagc tattgggcct ttacaaaaag cacgcagacg ggggtcggcg
    28861 cctgtaacac ttcgcccgtc tctgttagcc gaccggtctt cgggtccacg cggaactgat
    28921 aaatcgtgtc cgagtttt
//
LOCUS       DASREK010000138        16110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1553838, whole genome shotgun sequence.
ACCESSION   DASREK010000138 DASREK010000000
VERSION     DASREK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 16110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16110
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553838"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFA54_06985"
     CDS             <1..296
                     /locus_tag="VFA54_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZS50585.1"
                     /translation="NGRRYPEFFRINFSRCIFCGYCEEACPTYAIQLTPDFEMGEFRR
                     QNLVYEKEDLLIDGPGKYPGYNFWRVAGVKIGGKDKGEAQREAPPADVHSLLP"
     gene            302..895
                     /gene="nuoJ"
                     /locus_tag="VFA54_06990"
     CDS             302..895
                     /gene="nuoJ"
                     /locus_tag="VFA54_06990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZS50586.1"
                     /translation="MEATFYIASAVAVIATVLMLTRLNAVHALLYLIVSLLAVAVVFF
                     VAGAAFIAALEVIIYAGAIMVLFVFVVMMLNLGLRTEEMERRWQPSRAWIGPLALAAV
                     LLAELVYLLTAGSGPAQIAGSVEPKQVSIVLFGPYLLGVELASMLLLAGLVGAYHLGR
                     RAPEKPEDRYGADSDERRAAAGGDLVHAGSDRPARTP"
     gene            825..1118
                     /gene="nuoK"
                     /locus_tag="VFA54_06995"
     CDS             825..1118
                     /gene="nuoK"
                     /locus_tag="VFA54_06995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZS50587.1"
                     /translation="MRDGLLLAGILFTLGLIGLLVRRNLIFILMSLEVMLNAAGLAFV
                     IAGSRWHQADGQVVFLFILTMAAAEVSVGLALILQLYHRFKALDADAASEMRG"
     gene            1120..2994
                     /gene="nuoL"
                     /locus_tag="VFA54_07000"
     CDS             1120..2994
                     /gene="nuoL"
                     /locus_tag="VFA54_07000"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZS50588.1"
                     /translation="MLELLWLVLAVPFASALLLATLGPRFSRKAVAAAGVGSIGAAAV
                     LMILIAGRFVSSPPSGGAYAQVLWTWLSIAGFDPRIGLYLDALSLVMALVVTFVGFFI
                     HLYSAEFMIEDEGYSRFFCYMNLFVASMLTLLLADNLLLLFLGWEGVGLCSYLLIGFW
                     YQDPANGLAARKAFIVTRVGDTAMTLGLLLLFTRLGTLQIQELMRRAGDQWGPGSSLA
                     AIAAFLLLGGAIGKSAQLPLQTWLPDAMAGPTPTSALIHAATMVTAGVYLIARTHALY
                     LLAPQAQLAVGAVGAATLLAAGCSALTQHDIKRVLAYSTISQIGYMFLALGTGAWAAA
                     IFHFLTHAFFKALLFLGAGVIIEALHHEHSIFRMGGLRKELPLAFWTFLIAGCSLAGL
                     PLITAGFFSKDFILWGAWSAQQGRPAFWAAGMAGAFLTAIYTFRLIFLVFFGQARTPV
                     SKRPGWAMQVPLVVLAFFSIAAGYLKNPLAGYLQSALPPTLEVHTGAITETISASIAA
                     AVFAAGFYLAFWFFYRRRSLADAVASSAAGSALHGLWFSDWGMDWLYDRLFVQPLLWA
                     ARVNRGDFVDAFYTGLARLSELFYRGLSRTETGRVRWYAAGIAAGTVLFIAVVLFSRY
                     "
     gene            3007..4503
                     /gene="nuoM"
                     /locus_tag="VFA54_07005"
     CDS             3007..4503
                     /gene="nuoM"
                     /locus_tag="VFA54_07005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZS50589.1"
                     /translation="MILLWLIVILLGAGALAWVAARWNTEAPRWISLAAVSTDLLLLV
                     FTWTRSKRMESDSALWIEQVNFDWVPRFGIHFHLAMDGLSLLLLALTFFLGIMAVLAS
                     WTEIRDAVGFFHFNLMWILAGITSVFLALDLFLFYFAWELMLVPMYFLIAIWGHERRV
                     YAAVKFFLFTQLSGLLMLIAILALVFIHHRATGVYSFEYTDLLGSSLPAGADLWIMLG
                     FFVAFAVKLPMFPLHTWLPDAHTEAPTAGSVILAGLLLKTGAYGMLRFVAPMFPLAVS
                     EFAPAARVLAAIGILYGAVMAFAQTDLKRLIAYTSVSHLGFVLLGIFAWNPLALQGAM
                     MTMICHGFSTGALFILVGALQERTHTREMDRMGGLWSTAPRLSGAALFFALASLGLPG
                     LGDFIGEFLVLLGSYRTGVGITIAATIGVLAATFYALRMVQRAFHGPNANGWHIPDLT
                     PREGFVVAAMVVGLLWLGLYPQPVLNTFSPAMENLQRHASQPIVASRR"
     gene            4507..5931
                     /locus_tag="VFA54_07010"
     CDS             4507..5931
                     /locus_tag="VFA54_07010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZS50590.1"
                     /translation="MSGADFLALLPLILIAATSIVVMLAIAVARNHTLIAGLTVVGLT
                     GAFVSVWLVAPVAPRQVTPLLVIDRYALFYTALLAAAAIAVAALSHAYLHGRGIPAEE
                     LYLLLLLATLGCVVLVASSHFVSFFLGLEILSVSLYAMIAYLREWERPLEAGIKYLIL
                     AAASAAFLLFGMALIYADTGTMDFARIADIAAGRANLPLLLPGTALLVTGVGFKLALV
                     PFHLWTPDVYEGAPAPVTAFVATASKGAMFALLVRYFYRSGSQHPGIAAVFSIIALAS
                     MFAGNLLALLQANVKRILAYSSIAHLGYLLVAFLAGGPLGLEAISFYLVAYFITTLGA
                     FGVVAALSDRGRDADDLEDYRGLFWRRPGTAVVFTAMLLSLAGIPLTAGFIGKFYVVA
                     AGASGAKWALVLSLVVASAIGLYYYLRIVVMMYRRPPEGAAVRALAPASGFVLAMLTA
                     LLLWFGVYPTPLLNVIRAAVTMPQ"
     gene            6047..7576
                     /gene="zwf"
                     /locus_tag="VFA54_07015"
     CDS             6047..7576
                     /gene="zwf"
                     /locus_tag="VFA54_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZS50591.1"
                     /translation="MTTPQADIKGAGKAGDPCAMVIFGASGDLTKRKLLPALYNLARK
                     NLLPADFALIGFALDQLSDEDFRKQVQNNLREFGGAEENCRFCDWLVGRLYYLSGDFR
                     DSASYGRLKPLLDQVDQKHGTQGNRLYYLATTPALFAEVVSQLGAAGLTEETGGRWRR
                     VVIEKPFGHDLESARKLNAEIRKVLAEHQIYRIDHYLGKETVQNILVFRFSNGIFEPI
                     WNRRYIDHVQITVAETLGVEQRGAYYDHAGALRDMVPNHILQLVTLTAMEPPISFHAD
                     SVRDEQAKILHAIQPLAPEDVLTRAVRGQYGEGVIDGERVPAYRSEPHVAPDSQTETY
                     VALKLSIDNWRWADVPFYIRTGKRLARRVTEIGIQFRRAPLVLFRETPVDQLSPNLLV
                     LHIQPDEGISLRFGAKVPGPVVRVGSVNMDFNYEDYFGKEASTGYERLLHDCMIGDPT
                     LFQRADMVEAGWGVVEPILDVWKALPPRRFPNYPAGSWGPREADELLERDNRGWHISS
                     R"
     gene            7573..8553
                     /gene="glk"
                     /locus_tag="VFA54_07020"
     CDS             7573..8553
                     /gene="glk"
                     /locus_tag="VFA54_07020"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HZS50592.1"
                     /translation="MILAGDIGGTHTRLALFPQEPGAAPPILEVFSSREYASLAVIAR
                     KFLAGRNLRADIACFGIAGPVREGRAETSNLPWVIDSRELAGELGIEPVFLINDLEAN
                     AYGIAALAASDFAVLNAGAPSASGNQALIAAGTGLGEAGLVRETSGHRPFPSEGGHAD
                     FAPRNELEIELLRYLLARFDHVSYERVLSGPGLYAIYEFLRDTGRAAAPGPVAEEIAG
                     GDAAAVISKYGLEGSPEICKKTLDLFVSVYGAEAGNLALKVMATGGVFIGGGIAPKIL
                     PKLREPVFLRSFVSKGRLRKILEDIPVRVIMNEKAALLGAARFASLSTRL"
     gene            complement(8604..11876)
                     /locus_tag="VFA54_07025"
     CDS             complement(8604..11876)
                     /locus_tag="VFA54_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50593.1"
                     /translation="MLCVKKLFAIAGLFFMAIALILATSSPAAAQYTTANLGGVVTDA
                     TGAGVPDARVTVRNTETGFTQSTTTGADGAYVFPRLPVGAYELRVEKAGFTTYVQSGI
                     TLVVDQAANVPVTLQVGQVSNEVTVTGETELVTTRTATGSQVINQRPIVELPLNGRRP
                     ERLMYLSVGTVDSGRNSCRICGQGGVYPGEETPNINGAGLLSSSSSGQVNFQMDGADH
                     NDTYLNTSLPFPNPDAVQEFSLLSSNFTAEYGNAGGGIVNIVTRSGTNEIHGSLFEFL
                     RNGDLNARQFFAPTQDALKRNQFGGSAGAPIVKDKLFIFGTYQGTRVRNVPAGQVQFV
                     PTAAQRNGDFSAVSKQLIDPTTGQAFANNQIPTSRLSPPALNFLKNIPLPNGPNGQLT
                     FPGAPIAQTEDQFMVKPDYILGKQQISGHYFFTNFKAPPANVPGNVLADPNTGNQVRV
                     QNIAINHTYTVSPTFLFNTTFGLNRQRGGSLSSAPFSFRDMGVNILGPQDSTVKAPPE
                     LVVSVTGGFNINTNHFGQFDRGDFTIREVVTKIRGAHQFRFGGEAVRVSNHLINTFQM
                     AGNFTFNGQLSGNGLADFMLGQASSFTQGGGEFKNLKGTKWGFFVQDDWRVNQRLTLN
                     LGVRWDPYIPYYDRDGRVVCFQPNNTAKSQRYPNAPFGFFYGGDPGCPVAGVNPNWWN
                     VEPRIGFAYRLTQDGKTSLRGGAGIYYTPVPSGGTFNGYADTAPFAGTFTLNSVSFQD
                     PYGSKGLANPFPSNFGPQVPGPSFVFAPLNSIVNYFPLDYRIPELTVWSLRLERQIGR
                     DWVASVAYVGNKGTYLPINLQQNPAIYIPGASTVANTQDRRIYKTDGSIQYANPGGNS
                     EYNALQLNVEKRFSHGFTILANYAWSKSLDNLSNTNPFTLKNEHSLSQNDVPNNFKFS
                     GVWEVPGPKTGLLNKLLGGWQINPIVTKQSGFPFTVTSGADNSFSGVNADRADYKGGG
                     SAQLPYDRPHGQQVSQWFDTSKFTVNAPGTFGNAGRFILRGPRFFNTDLGILKGTKIT
                     ERVNLQFRAEFFDVFNNVNFQLPNSNMSSAQVGRITAVVQDNFQLPNSERIIQFGLKL
                     TF"
     gene            12315..13133
                     /gene="ccsA"
                     /locus_tag="VFA54_07030"
     CDS             12315..13133
                     /gene="ccsA"
                     /locus_tag="VFA54_07030"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZS50594.1"
                     /translation="MGETSVFWLRVTLALYSLGLGHALLTVIKRRQRLFAAALVAFCV
                     AAVFHLVSIVEEGFAVGRFPVINLYQSSSLIGFLITLLFLLAYWRYKYDSLAVFIFPI
                     VFIFTLVASLGHPVEPWSSPVLRSSWLFVHVVLALLGYAALFLTCVAAIMYLIQEREL
                     KSKKPRAFYHWLPPLGALDELGYRTMAVGFVLITLGLIAGSCWAFIEWGTRWVVDPKI
                     ILYFVTWGVYLILIFTRWTIGWRGRKAAYLAIFGFCCAALTWMVDSGVHSFLKR"
     gene            13133..14398
                     /gene="hemA"
                     /locus_tag="VFA54_07035"
     CDS             13133..14398
                     /gene="hemA"
                     /locus_tag="VFA54_07035"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZS50595.1"
                     /translation="MNLLVVGLSHKTAPVDVREQMALPETEMAGVLTALTSRPGIAEA
                     LIFSTCNRVEFTVRAEPETDAVAEVYSFLANDCRRPIEKLERHLYRYSQSEAIRHLFR
                     VAASLDSMVVGEPQILGQVKDAYAIAKAAGALGGPLEEIVSRSLHVAKRVRSETGIGQ
                     MAVSVSYVAVELARKIFGTLAGHTVLILGSGKMSELAARHLRRSGAPAVFVANRTYEK
                     AVELASRFEGRPVPFDQLFEFLQHVDIVISSTGAPGYIITRPRAEALMAARKNRPMFL
                     IDIAVPRDIDPEVNKIDNLFVYDIDDLQQVADSNKREREREAERAEAIVAEEVASMMA
                     RLKAHEIAPTIVSLQSELDRIRRAEMERHRQRLGSLTPEQQRAVDELTRGIINKIAHT
                     PISQLKSLANHPDGLHVIDVVKRVFNLKD"
     gene            14526..15413
                     /gene="hemC"
                     /locus_tag="VFA54_07040"
     CDS             14526..15413
                     /gene="hemC"
                     /locus_tag="VFA54_07040"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZS50596.1"
                     /translation="MIIGSRGSQLALRQARWVAAHLPGAEVEVIKTTGDQMTASPFSA
                     GGAKALFTKEIEEALLGGRIDLAVHSLKDMPVQMNSGLTIAAVPEREEVRDALVGSKL
                     SSLPEGALVGTSSLRRAAQLRYLRPDLRVENLRGNVDTRLRKLEEGRYTAIVLAAAGL
                     RRLSLEARIAELLDPELMCPAVGQGALAIETRAGDERMRTILAPLDDPRARAEVTAER
                     AMLAELGGGCQVPIGGLARVRDEELRLIAVVASPDGQQLIRVSVEGPASDPATVGRRA
                     AHELLVRGANKILEQVYTR"
     gene            15410..>16110
                     /gene="cobA"
                     /locus_tag="VFA54_07045"
     CDS             15410..>16110
                     /gene="cobA"
                     /locus_tag="VFA54_07045"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZS50597.1"
                     /translation="MSGGETGKAARIPPVALVGAGPGDPELITLKGLECIRQADVILY
                     DNLAPAALLDQARPCCIKTYVGKKRTEHALTQDQINRLMIEHARAGRRVVRLKGGDPF
                     LFGRGGEEAQALAAADVPFQVVPGVSSAMGAAAYAGVPLTHREHTSAVTFVSGHEVDK
                     IDWTRVGASETIVVFMGLVNCSEIAQRLIAGGRSPSTPAIAVRWATRPDQRVVSGTLA
                     NLGRKIAEAALKPPT"
BASE COUNT         2736 a         4845 c         4993 g         3536 t
ORIGIN      
        1 agaacgggcg gcgttaccct gagtttttcc gcattaattt ttcgcggtgc attttttgcg
       61 gctactgtga ggaggcgtgc cctacctatg ccatccagct cacccccgat ttcgagatgg
      121 gcgagttccg gcggcagaac ctggtgtatg aaaaggaaga tttgctcatc gacggccccg
      181 gtaaataccc gggctacaat ttctggcggg ttgcgggcgt gaaaatcggc ggcaaagaca
      241 agggagaggc ccagcgcgaa gcgccgccgg ccgacgtgca cagcctgctg ccctgaagac
      301 catggaagcg acgttctaca ttgcaagtgc tgtggccgtg atcgccactg tcctgatgct
      361 gacgcgcctg aacgcggtgc atgccctgct atatcttatc gtctcgctgc ttgccgtcgc
      421 cgtggtgttc ttcgtggccg gcgctgcgtt catcgccgct ctcgaagtca ttatttacgc
      481 cggcgccatc atggtgcttt ttgtcttcgt cgtgatgatg ctgaacctcg gcctgcgcac
      541 ggaagaaatg gagcgccgat ggcagccttc gcgcgcctgg atcgggcctc tcgccctggc
      601 cgcagtgctg ctcgcggagc tggtgtatct tctcaccgcc ggcagcgggc cggcccagat
      661 cgccgggagc gtagagccga agcaagtgag cattgttttg ttcgggccat atctgctggg
      721 agtggagctc gcatcgatgt tgttgctggc gggactcgtg ggcgcctatc acctggggcg
      781 gcgcgcgcct gaaaaaccgg aggatcgcta tggcgcagat tccgatgaga gacgggctgc
      841 tgctggcggg gatcttgttc acgctgggtc tgatcggcct gctcgtacgc cgtaatctga
      901 ttttcattct gatgtcgctc gaggtcatgc tgaacgcggc cggcctggcg tttgtaatcg
      961 ccggctcgcg ctggcatcag gccgacggcc aggtggtctt tctttttatt ctgaccatgg
     1021 cggcggcgga ggtctccgtg gggctggcgc tgatcttgca gctttatcat cgattcaagg
     1081 ccctggacgc cgatgccgcg agcgagatga gaggatagcg tgctcgaatt gctctggctg
     1141 gttctcgccg ttccttttgc cagcgctctg ctgctggcga cgctcggccc gcgtttttca
     1201 cgcaaagcgg tcgccgccgc cggcgtcgga tcgatcggag cggcagccgt tctcatgatt
     1261 ctgatcgctg gccgatttgt cagttcgccg ccttcgggcg gagcatacgc ccaggtgctt
     1321 tggacctggc tgagcatcgc aggcttcgat ccccgaatcg ggctctatct ggatgcgctc
     1381 tcgctcgtga tggcgctggt ggtgaccttt gtcggcttct ttattcacct atactccgcc
     1441 gagttcatga tcgaagacga gggctacagc cggttcttct gctacatgaa cctgtttgtg
     1501 gcctcgatgc tcacgcttct actcgccgat aacctgctcc tgctctttct gggatgggaa
     1561 ggagtgggct tgtgcagcta ccttctgatc gggttctggt atcaggaccc ggccaacggc
     1621 ctggcggcgc gcaaggcatt catcgtcact cgcgtcggcg acacggccat gactctcggc
     1681 ctgcttctgc ttttcaccag gctggggacg ctgcaaatcc aggaactgat gcggcgggcg
     1741 ggcgaccagt gggggccggg atcgagcctc gccgcgattg cggcgtttct gctgctgggg
     1801 ggcgcaatcg gaaagtccgc ccagcttccc ctgcaaacct ggctgccgga tgcgatggcc
     1861 ggtcccacgc ccaccagcgc cctgattcac gccgccacaa tggtgaccgc cggtgtctat
     1921 ttgatcgccc ggacgcacgc tctttatctg ctggctccgc aggcgcagct tgccgtaggc
     1981 gccgtgggtg cggcgacgct gcttgccgcc ggatgcagcg ctcttactca gcacgatatc
     2041 aagcgcgtgc tcgcctactc caccatcagc cagatcggct acatgtttct ggctctcggg
     2101 acgggcgcgt gggcagccgc gattttccac ttcttaacgc acgcgttctt caaggccctg
     2161 ttgttcctgg gagccggagt gattatcgaa gccctgcacc acgagcacag tattttccgc
     2221 atgggcggat tacgcaagga gctgcccctg gcgttctgga cgtttctcat cgccggttgc
     2281 tcgctggcgg ggcttccctt gatcaccgcc ggattcttca gcaaggattt cattctttgg
     2341 ggcgcctgga gcgcgcagca ggggcgcccg gccttttggg cggccggaat ggccggagcg
     2401 ttccttacgg ctatttacac attccgtttg atttttttgg tcttcttcgg ccaagcgcgc
     2461 accccggtga gcaagcgccc gggatgggcg atgcaggttc cgctggtcgt gctggcgttt
     2521 ttctcgatcg cggccggtta tttgaaaaac cccctggccg ggtatctgca atccgctttg
     2581 ccgcccacgc tggaggtgca taccggcgcg ataacggaga caatatccgc gtcgatcgct
     2641 gcggccgtgt ttgccgccgg tttctatctc gctttttggt ttttttaccg aagacgcagc
     2701 ttggccgatg ctgtggcatc ttcggcggca ggttccgctc tgcacgggct gtggttttcc
     2761 gattggggca tggactggct gtatgaccgg ctctttgtcc agccgctcct gtgggccgcc
     2821 cgcgtcaaca gaggggattt cgtggacgca ttttataccg ggctggctcg cttgagcgag
     2881 ctcttttatc gcgggctcag ccgcaccgaa accggacgcg tgcgctggta tgcggcgggt
     2941 atcgccgccg gaacggtctt gttcattgcc gtcgtattgt tttccaggta ctgacgcacc
     3001 gattctatga ttctgctttg gctcatcgtc attttgctcg gggcgggcgc gctggcctgg
     3061 gtggcagcgc gttggaatac cgaggcgccc cgctggatat ctctcgccgc cgtttcgacg
     3121 gacttgctgc tgctggtttt cacctggacc cgaagcaagc ggatggaatc tgacagcgct
     3181 ttgtggatcg agcaggtgaa tttcgactgg gtgccccggt tcggcattca ttttcatctg
     3241 gcgatggacg gcctcagcct cctgctgctc gcgcttacat tcttcctggg catcatggcg
     3301 gtgcttgcct cgtggacgga aattcgcgat gcggtcggct ttttccactt caatttgatg
     3361 tggattctag cgggaatcac cagcgtcttc cttgcgctcg atctgtttct gttctatttc
     3421 gcctgggagc tgatgttagt cccgatgtat tttctgatcg cgatttgggg ccatgagcgg
     3481 cgcgtgtatg cggccgtcaa gtttttcttg ttcactcagc tcagcgggct gctgatgttg
     3541 atcgccatcc tggcgcttgt cttcatccac caccgggcca cgggcgttta cagcttcgag
     3601 tacacggatc tgctcggatc cagccttcct gccggagcgg atctctggat catgctcggc
     3661 tttttcgtcg ccttcgcggt gaagctgccc atgttcccgc tgcacacgtg gctgcccgac
     3721 gctcacaccg aggcgccgac cgccggcagc gtgattcttg cggggcttct gctcaagacc
     3781 ggggcctacg gaatgctgcg tttcgtggct ccgatgtttc ccctggcggt gagcgagttt
     3841 gcgccggccg cccgcgtcct ggcggccatc ggcattctct acggcgcggt gatggcgttc
     3901 gcccagacgg acctgaagcg gctgatcgcc tacacaagcg tcagccacct gggctttgtt
     3961 ttgctaggca ttttcgcgtg gaaccctctc gcgctccaag gggccatgat gaccatgatc
     4021 tgccacggct tcagcacggg cgcccttttc attctggtcg gcgcgctcca ggaacgcact
     4081 catacgcggg agatggaccg catgggcgga ctgtggtcca cggcgccccg tttgagcggg
     4141 gccgcgctct tcttcgcgct ggcttcgcta ggcctgccgg gccttggcga tttcattgga
     4201 gaattcctgg tgctgctggg aagctaccgc acgggcgtcg gaattactat agcggccaca
     4261 atcggcgtgc tggctgcgac cttctatgcc ctgcgcatgg tgcagcgggc gtttcatggg
     4321 ccgaatgcga acggctggca tattcctgat ttgactccgc gcgagggatt cgtagtagcg
     4381 gcgatggtgg tcggtctgct gtggctcggt ctttatccgc agccggtgct caacacgttt
     4441 tctccggcga tggagaattt gcagcgccat gccagccagc ccatcgtcgc gtcgaggaga
     4501 tgatcaatga gcggcgctga tttcctcgca ttgctgccgc tgattcttat cgccgcgact
     4561 tccatcgtgg tgatgctggc catcgccgtt gcccgcaacc atacgctgat tgccggcttg
     4621 accgtggtcg gcctgaccgg cgcgttcgtt tcggtgtggc tagtggcgcc ggtcgcgccc
     4681 cggcaagtga cgccgctgct ggtgatcgac cgttacgcgc ttttctacac ggcgctgctt
     4741 gctgcggccg cgatcgcggt cgcagcgctc agccatgcct acctgcatgg ccggggcatc
     4801 ccggctgaag aactatacct gctgctgctg ctggcaacgc tcggctgcgt ggtcctggtg
     4861 gcgagttctc actttgtttc gttctttcta ggcctcgaga tcctcagcgt ctcgctttac
     4921 gcaatgatcg cgtacttgcg cgaatgggag cggcccttag aggcaggcat caagtatttg
     4981 atcttggcgg cggcttcggc ggcctttctg ctttttggca tggctttgat ctacgcggac
     5041 accggaacaa tggattttgc gcgcatcgcc gatatcgccg ccggccgggc caacctgccg
     5101 ctgctgctgc cgggaacggc gctcctcgtc acaggagtgg gattcaagct cgcgttagtg
     5161 ccatttcatt tgtggacccc agatgtatac gagggcgctc cagcgccagt cacggcgttc
     5221 gtagccacgg cttccaaagg agccatgttc gctctcctcg tgcggtactt ctatcgctcc
     5281 ggctcgcagc atccgggcat tgccgccgtc ttctcgatta tcgcgctcgc ctcgatgttc
     5341 gccggcaatt tgctcgcact gttgcaggct aacgtaaagc gtattctggc ttactcctcc
     5401 atcgcccatc tggggtacct gctggtagct tttctggccg gcgggccgtt gggcctggaa
     5461 gcgatcagct tctacctggt ggcgtatttc atcaccactc tgggcgcatt cggcgtcgtc
     5521 gccgcccttt cggatcgcgg acgcgatgcg gatgatctcg aagattaccg cggccttttc
     5581 tggcggcggc cgggcacagc tgttgttttt accgcgatgc tgctttctct agccggcatt
     5641 ccgctgaccg ccgggtttat cgggaaattt tatgtggttg ccgccggagc ctccggcgcg
     5701 aagtgggccc tggtgctgag cctcgtggtg gcgagcgcga tcgggctcta ctactatctc
     5761 cgcatcgtgg tcatgatgta tcgcaggccc cccgaggggg ccgcggtccg cgctctggct
     5821 cctgccagcg gctttgttct ggctatgcta acggctttgt tgttgtggtt tggcgtttac
     5881 cccacgccgt tgctgaatgt gatcagagca gcggtgacca tgccgcaata acgccggtga
     5941 tttccggctg catggaaggc cgaggcgaaa ctcgcggcac gctccggaac gccgcgaaca
     6001 atcgtcgggc aagacggcta gcgcaaggac gcaacggtaa atccgcatga caacaccgca
     6061 agctgatatc aaaggtgcag gcaaagcggg agacccctgc gccatggtga ttttcggcgc
     6121 ttccggcgat ctgaccaagc ggaagctgct gcctgcgctc tataatttgg cccgcaagaa
     6181 ccttctgccc gccgatttcg ccctgattgg cttcgcactg gaccagctca gcgacgaaga
     6241 tttcagaaag caggtgcaaa acaatcttcg ggagtttggc ggcgcagagg aaaattgccg
     6301 tttttgcgac tggctcgtcg ggcggctcta ttatttgagc ggcgacttcc gggactcagc
     6361 ttcctacggc cggcttaaac cgctgctcga ccaagtggac cagaagcatg gcacacaggg
     6421 caaccggctc tattacctgg ccaccacacc cgcgctgttt gccgaggttg tctcacagct
     6481 cggagccgcc ggcctgacgg aagagacggg gggccgctgg cggcgtgtgg tgatcgaaaa
     6541 accgttcggc cacgatttgg aatcggcccg caagctcaat gcggagataa gaaaagtgct
     6601 ggccgagcat cagatttacc gtatcgatca ttacttgggc aaggagaccg tccagaacat
     6661 tctggttttc cgtttttcca acggcatttt cgagccgatc tggaaccggc gatatatcga
     6721 ccacgtgcag atcacggtgg ccgaaaccct gggcgtggaa cagcgcggcg cctattatga
     6781 ccacgccggc gcgctgcgcg acatggtgcc gaaccatatc ctgcaactgg tcacgcttac
     6841 ggccatggag ccgccgattt cgtttcatgc cgactcggtt cgcgacgagc aggcgaaaat
     6901 cctgcacgcc atccaaccgc ttgccccgga agacgttttg actcgcgccg tgcggggcca
     6961 atatggggaa ggcgtgattg acggcgagcg cgttccagct taccgctccg agccgcacgt
     7021 agcgcctgat tcgcagaccg aaacctatgt cgcgctgaag ctctccattg acaactggcg
     7081 ctgggcggac gtgccgttct atattcgaac cggcaaacgc ctcgcccgcc gcgttacgga
     7141 gatcggcatt cagttccgcc gcgctcctct cgtccttttt cgtgagactc ccgtcgatca
     7201 gttgagtccc aatcttcttg tgctgcacat ccagccggat gaaggcattt ccctgcggtt
     7261 tggcgcgaaa gttcccggcc cggttgtgcg cgtgggttcg gtcaacatgg atttcaatta
     7321 tgaggactat ttcggaaaag aggccagcac cggctacgag cgcctcttac acgattgcat
     7381 gatcggcgac cctacgctgt tccagcgcgc tgacatggtg gaggcgggct ggggcgtggt
     7441 cgagccgatt ctggacgtct ggaaagccct tccgccgcgg cgatttccta attatccggc
     7501 ggggtcttgg gggccgcggg aggccgacga gctgctcgag cgcgacaatc gcggctggca
     7561 catatcatcc cgatgatttt ggctggtgat atcggcggaa ctcacacccg actcgccttg
     7621 ttcccgcagg aacccggcgc cgcacctccg attctggagg ttttttccag ccgggaatac
     7681 gcgagcctgg cggtgatcgc gcggaaattc ctcgcaggcc gcaacctccg ggccgacatc
     7741 gcctgcttcg gcatcgccgg cccggtgcgg gagggtcgcg ccgagacgtc caatttgccc
     7801 tgggtcatcg attcgcgcga gttggccggg gagctcggca tagagccggt ttttctcatc
     7861 aacgacctcg aagccaatgc ctatgggatc gccgcgctcg ctgcttccga tttcgccgtt
     7921 ctgaacgcag gtgcgccatc ggcttcggga aaccaggcct taatcgccgc aggcaccggt
     7981 ttaggagagg cgggcctggt gcgggagacg tccggccacc gcccgtttcc ttctgaaggg
     8041 ggtcacgctg attttgcgcc ccgcaacgaa ctggaaatcg aactgctccg ctatctgctg
     8101 gcccgcttcg atcacgtcag ctatgaaaga gttctctcgg gccccgggtt gtacgccatt
     8161 tacgaattcc tccgggatac cgggcgcgca gcagcgcccg ggccggtagc agaggaaatc
     8221 gccggtggcg acgcggcggc ggtgatttcc aaatacggct tggaaggatc gccggagatt
     8281 tgcaagaaga cgctcgattt gttcgtgtcc gtctatggcg ccgaagccgg caatctggcg
     8341 ttgaaggtga tggccacggg cggggtcttt atcgggggcg gcatcgcgcc gaagattctt
     8401 cccaagctgc gggaaccagt tttcctcagg tcatttgtat ccaagggccg cctccgcaag
     8461 atactggagg atattcccgt tcgcgtaatc atgaatgaga aggccgcgct gctcggagca
     8521 gctcggttcg cctccctgag cacgcggctt tgagggcttc cgttatatct gaggcgccgt
     8581 agcgcgggat aacttattcc gtattagaac gtcagcttca gcccgaactg gatgatgcgc
     8641 tccgagttgg gaagctggaa gttgtcctgc accactgccg tgatccttcc cacctgagcc
     8701 gacgacatat tgctgttggg aagctgaaaa ttgacgttgt tgaaaacatc gaagaactca
     8761 gcgcggaatt gcaggttgac ccgctcggta attttcgttc ctttcaaaat tccgaggtcg
     8821 gtgttgaaaa accgcggtcc ccgcagaatg aaccttccgg cgttaccaaa ggtccccggg
     8881 gcgttcacgg tgaatttcga agtgtcgaac cattgcgaca cctgctggcc gtgcggccgg
     8941 tcatacggga gctgcgcgct gccgccgccc ttgtagtcgg cccggtccgc gttcacgccg
     9001 ctgaaggaat tatcggcgcc gctggtcacg gtgaacggaa aaccgctctg cttggtgacg
     9061 atgggattga tctgccagcc gcccagcagc ttgttcagca atcccgtttt gggccccgga
     9121 acctcccaca cgccggaaaa cttgaaattg ttgggcacgt cgttctgtga aaggctgtgc
     9181 tcattcttta gcgtgaacgg gttcgtgttg ctcaggttgt caaggctctt cgaccacgcg
     9241 tagttcgcca aaatcgtaaa tccgtgcgag aagcgcttct ccacgttcaa ctgcaacgca
     9301 ttgtattcgc tgttgccgcc cggattggcg tactgaatgc ttccatcggt cttgtaaatg
     9361 cgccggtcct gggtattggc cacagtggaa gcgcccggaa tatagatcgc cgggttttgc
     9421 tgcaagttta tcggcaaata cgtgcccttg ttgcccacgt acgccacgct tgccacccag
     9481 tcccggccga tttgccgttc aagccgcagg ctccagaccg ttaattcggg gatccggtaa
     9541 tccagcggga agtaatttac gatgctgttc agaggcgcga agacgaagct cggccccggc
     9601 acctgcggtc cgaagttgct cggaaacggg ttggccaagc ctttgcttcc ataaggatcc
     9661 tggaaggaaa ccgaattcaa cgtaaacgtg ccggcaaagg gggccgtatc ggcgtagcca
     9721 ttgaaggttc cgcccgacgg aacaggcgtg tagtaaatac ctgccccgcc ccgcaggctc
     9781 gtttttccat cctgcgtcaa ccggtaagca aagccaatgc gcggctcgac attccaccaa
     9841 ttggggttga cgcccgccac cgggcagccc ggatcgccgc cgtaaaagaa tccgaacggc
     9901 gcgttcggat aacgctgcga cttcgcagtg ttgttcggct ggaagcagac cacacgccca
     9961 tcgcgatcat aataaggaat gtagggatcc cagcgcacgc cgaggttcag cgtcagccgt
    10021 tggttgactc tccagtcatc ctggacaaag aagccccact ttgtcccttt cagatttttg
    10081 aactcaccgc ctccctgggt gaacgaggac gcctggccga gcatgaagtc ggcgaggccg
    10141 ttgcctgata attgcccgtt gaaagtgaag ttcccggcca tctggaaggt attgatcaga
    10201 tggttcgaga cgcgcacagc ttcgccgccg aagcggaatt ggtgggcgcc gcggattttg
    10261 gtcaccactt cgcgaatcgt gaagtcgccg cgatcaaact ggccgaagtg attcgtattg
    10321 atgttgaagc ctccggttac tgatacaaca agttccgggg gcgccttcac cgtagaatcc
    10381 tgcggtccca ggatattgac acccatgtcg cggaagctga aaggcgcgct ggaaagagat
    10441 ccgccgcgct gccggttcag gccgaacgta gtattgaaca gaaaggtggg agaaaccgtg
    10501 taagtgtggt tgatagcgat gttctggacg cgcacctggt tgccggtgtt gggatcggcg
    10561 agaacgtttc ccggcacgtt cgccggcggg gccttgaaat tggtgaagaa gtaatgcccg
    10621 ctgatctgct gcttcccaag gatataatcc ggcttcacca tgaattgatc ttccgtctgc
    10681 gcgatcggcg cgccggggaa ggtcaattgg ccgttcgggc cgtttggcag cggaatgttt
    10741 ttcaaaaagt tcagcgccgg cgggctcagc cggctggtgg gaatctgatt gttcgcgaac
    10801 gcctgcccgg tggtcggatc gatcagttgc ttggaaacgg ccgagaaatc tccgttgcgc
    10861 tgcgccgccg tcggcacaaa ctgcacctgg ccggccggca cgtttctgac tcgggtgccc
    10921 tgatacgtcc cgaaaatgaa cagcttgtct tttacgatcg gcgcgccggc gctgccgccg
    10981 aattgattgc gcttcagagc gtcctgggtt ggcgcgaaga actgccgcgc gttcagatcc
    11041 ccgttccgga gaaactcgaa aagcgacccg tggatctcgt tcgtccccga ccgcgtaacg
    11101 atatttacga tgccgccgcc ggcgtttcca tattcggcgg taaagttgct tgagagaagg
    11161 ctgaattctt gcaccgcgtc ggggttggga aacggcaagc tggtattcag gtatgtatca
    11221 ttgtgatcgg cgccgtccat ttggaagttg acctggcctg agctggacga gctgagcagg
    11281 ccggctccgt tgatgttcgg cgtttcctct ccgggatata cgccgccctg gccgcagatg
    11341 cggcaactgt ttctgccgga atccactgtg cccacggaga gatacatgag ccgctccggc
    11401 cttcttccat tcagcgggag ctccacaatc ggcctctgat tgatcacctg gcttccggtc
    11461 gccgtgcgcg tcgtgaccag ctcggtttct ccggttacgg tcacttcgtt cgatacctgg
    11521 ccgacttgaa gtgtaaccgg cacgttcgcc gcctggtcaa ccaccaacgt gatacccgac
    11581 tgcacgtaag tggtgaatcc cgctttttcg actcgcaatt cgtaggcgcc taccggcagc
    11641 cggggaaaca cgtacgcgcc atcggcgccg gtcgtggtgg actgcgtaaa tccggtttcc
    11701 gtgttgcgga ccgtcactct ggcgtcgggc acgccggcgc ccgtcgcatc cgttacgact
    11761 ccacccaggt tcgcggtcgt atattgcgcc gccgccggcg aactggtcgc caggatcagc
    11821 gcgatcgcca tgaaaaacaa tcctgcaatc gcgaaaagct tttttacgca aagcatatat
    11881 gttatccctc tgtccagccg tggctggacc acgaacagtt ttgtcaatct ccgggcacga
    11941 gaaaacctgc tgcaacgatg gcaggacggc gctacccggc ggccgcacca tggaagcgcc
    12001 tgctaacttg acagcgcttc ggcccctaaa tatgcagaga tagtttcgca tggaaaaaga
    12061 tgggagtcaa tgcagggcgg ccgcagcgtg ggttaaccca tgttgcgctg cggcgcctgg
    12121 cggtatagcg aggcagcctc agaatggagc cgccgggccg gcgggcctgt tgtctgaggg
    12181 aaggcgcggt gcttgtgcga acggccatgg ccgcctttgc gaaacgcctt cccggcgctg
    12241 gcgatccggg cctctcgtcc tgtgggggtc ctgcgcgcga cgcggcggtc tcccggtgaa
    12301 atataatgaa tcctatgggt gaaaccagcg tattttggct gcgggtgacg ctggctctgt
    12361 actcgcttgg cctcggccat gcgctgctga cggtgatcaa gcgccgccag aggctgttcg
    12421 ccgcagcgct ggtcgccttc tgtgtcgcag ccgtctttca cctcgtctcc attgtggagg
    12481 agggcttcgc cgtcgggcgg ttcccggtaa ttaatctata tcagtcttcg tcgttgatcg
    12541 gattcctgat cacgctgctg ttcttgcttg cctactggcg atacaagtac gacagcctgg
    12601 ctgttttcat tttccccatc gtttttatct ttaccctggt ggccagcctc ggccacccgg
    12661 tcgagccctg gtcaagcccc gtactgcgaa gctcctggct cttcgtgcac gtagtgctcg
    12721 cgctgctcgg gtacgcggcg ctgtttctga cctgcgtggc cgcgatcatg tatctgatcc
    12781 aggagcggga actgaagtcc aagaagccgc gcgcgtttta ccactggctg ccgccgctcg
    12841 gcgcgctcga cgaattgggg taccggacga tggcggtagg ctttgtcctg attacgctgg
    12901 gactgatcgc gggctcctgc tgggccttta tcgaatgggg gactcgctgg gtggtggacc
    12961 ccaagattat tctgtacttc gtcacgtggg gcgtatatct gatcctgata ttcacccgct
    13021 ggacgattgg ctggcgcggg cgcaaggccg cgtatctcgc catctttggc ttttgctgtg
    13081 cggcgctcac gtggatggtt gacagcggcg tgcactcgtt cctgaaacgc tgatgaattt
    13141 attggtcgtc ggattgagcc acaagaccgc gccggttgac gtgcgggagc agatggctct
    13201 gcccgagacg gaaatggccg gcgttttgac cgctctcacg tcccgcccgg gaatcgccga
    13261 agccctcatt ttctctactt gcaaccgggt ggaattcacc gtgcgcgccg agccggaaac
    13321 cgacgccgta gccgaggtct attccttcct tgccaatgat tgccggcgtc cgatcgaaaa
    13381 gctggaacgg cacctctatc gctacagcca gagtgaagcc atccgccatc tgttccgggt
    13441 ggctgccagt ctggattcca tggtcgtcgg cgaaccgcag atcctgggcc aggtgaaaga
    13501 cgcgtacgcg atcgcaaagg ccgccggggc gctgggcggg ccgctcgaag agatcgtcag
    13561 ccggtcgctg cacgtagcca aacgcgtgcg cagcgagacc ggcatcggcc agatggccgt
    13621 ctcggtcagt tacgtggctg tggaactggc gcggaagatc ttcggcacgc tggccggcca
    13681 cacagtcctg attctgggct cgggcaaaat gtcggaactc gctgcccggc atctgcgccg
    13741 ctctggtgcg cccgcggttt tcgtcgccaa ccgtacgtat gagaaagccg tcgagctcgc
    13801 gtcccgcttc gaaggccggc cggtgccgtt cgatcagcta ttcgagtttc tgcaacacgt
    13861 ggacatcgta atcagctcga ccggagcgcc cggctatatc atcacccggc cccgcgctga
    13921 ggcactgatg gccgcccgca agaaccgccc gatgtttctg atcgacatcg ccgtgccgcg
    13981 ggacatcgat cccgaagtca ataagatcga caacctcttt gtttacgaca ttgacgatct
    14041 ccagcaggtg gccgattcca acaagcgcga gcgggagcgc gaggcggagc gggccgaagc
    14101 catcgtcgcc gaggaagtgg caagcatgat ggcccgtctt aaggcgcacg agattgcgcc
    14161 cacgatcgtc tctttgcaat cggagctcga ccgcatccgg cgggcggaga tggagcggca
    14221 ccggcagcgc ctgggaagcc tcacgcccga acagcagcgc gcggtggacg agctgactcg
    14281 cggcatcatc aacaagatcg cgcacacccc catcagccaa ctgaagagcc tggccaatca
    14341 ccccgacggt ctgcatgtga tcgacgtcgt gaagcgcgtt tttaatttaa aagattgaaa
    14401 gccaaaaata gaggcaaacg gacgccgatg aaaactactg cgaggctacc gcgcgcggcc
    14461 tgccgctccg gggcggcgtg cggaaggctt ttgcttttcc cggcgtatcg ctgcggtttt
    14521 ttccgatgat catcggatcg cgcggttcgc aattggcgct gcggcaggcc cgctgggtgg
    14581 cggcgcacct gccgggagcc gaagtcgaag tcatcaaaac caccggcgac cagatgacgg
    14641 cttctccgtt ctctgccggc ggcgccaagg ccttattcac gaaagagatc gaggaagcgc
    14701 tgctcggcgg ccgcatcgat ctggcggtgc acagcctcaa ggatatgccg gtccagatga
    14761 actccggtct caccatagcc gccgtccccg aacgcgagga agtgcgcgat gcgctggttg
    14821 gctccaagct ttccagcttg ccggaggggg ccctggtggg taccagttcg ctgcgcagag
    14881 cggcgcagct tcgttatctg cggccggatc tccgcgtgga aaatcttcgc ggaaacgtgg
    14941 acacgcgcct gcggaaactg gaggaaggcc gttacactgc aatcgtgctc gccgccgctg
    15001 gcctgcgacg gctttcgctg gaagcccgca ttgccgagct gctcgacccc gagctcatgt
    15061 gcccggcggt aggccaggga gccctggcga tcgagacgcg agccggcgat gaacgcatgc
    15121 gcacgatttt ggcgcccctc gacgatcccc gcgcccgcgc cgaggtgact gcggagcgtg
    15181 cgatgttggc cgaactgggc gggggctgcc aggttccaat cggcggcttg gcccgcgtgc
    15241 gagacgagga gcttcgcctg atcgccgtgg ttgcttcgcc agatggacag caactgatcc
    15301 gagtctcggt ggaaggcccg gcttccgatc ctgccactgt aggccgccgg gccgcgcacg
    15361 aactgctggt gcgaggggcg aacaaaatcc tcgagcaggt gtacacgcgg tgagcggcgg
    15421 cgaaaccggc aaagcggcgc ggatcccgcc ggtcgctctg gtaggggcgg gacccggcga
    15481 ccccgagttg atcaccctca aaggtctgga gtgcatccgg caggccgatg taattctgta
    15541 tgacaacctt gccccggctg cactgctcga ccaagcccgg ccctgctgca tcaagaccta
    15601 cgtgggcaaa aagcgcaccg agcatgccct cactcaggac cagatcaacc gtctcatgat
    15661 cgagcacgcc cgggccggcc ggcgtgttgt acgcctcaag ggcggcgatc cgtttctttt
    15721 cggccgtggc ggagaagagg cgcaagcgct ggctgcggcg gacgtaccct tccaagtcgt
    15781 tcccggcgtg agttccgcga tgggcgcggc cgcctatgcc ggcgtcccgc tcactcaccg
    15841 tgagcatact tcggccgtca cgttcgtaag cgggcatgag gtggataaga tcgactggac
    15901 tcgcgtcggc gcttcggaga ccatcgtcgt gtttatgggc ctggtgaact gctcggagat
    15961 cgcccagcgc ctcatcgccg gaggccgctc gccatccacg cccgccatcg ccgtccgttg
    16021 ggccacgcgc cccgatcagc gcgtcgtatc cggcacgtta gcgaatctcg ggcggaaaat
    16081 tgcggaggcc gccctcaagc cgccgaccat
//
LOCUS       DASREK010000139         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_742357, whole genome shotgun sequence.
ACCESSION   DASREK010000139 DASREK010000000
VERSION     DASREK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742357"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2109
                     /locus_tag="VFA54_07050"
     CDS             <1..2109
                     /locus_tag="VFA54_07050"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZS50598.1"
                     /translation="FSLLEFTIMGRRCRRCLATGLVAVSLAWAAESDWQQRFRGLPQP
                     DHIREYIRAMTEEPHHAGSPGSKKVAEYVLSKFREWGLEAQIEEFEALLPTPNDRTLE
                     LIEPQQFTARLKEPAIPEDKDSSDPGQLPTFNAYSPDGDVTGQVVYVNHGVVEDYERL
                     ARMGVDVKGKIVLARYGAGWRGVKPKLAWEHGAIGCLIYSDPREDGYFQGDVYPGGPF
                     RPAEGVQRGSVIDMPIYPGDPLTPGWASERGGRKLPLAEARTLPKIPVFPISYADATP
                     ILRNMAGPVAPEPWRGALPLTYHVGPGPATVHLKLSFDWQTRPIYNVIARIPGSDFPD
                     QWVIYGNHHDAWVNGARDPASGNAALLETARGLSELLKQGWKPRRTIILASWDAEEWG
                     LIGSTEWGEKHAEELRQKAVVYINSDGNSRGLLQASGSHSLEHLLNEVARSVPDPQTG
                     KPLWEAMRSRRLQEASDSEKKKAIASRSDLGIGALGAGSDYVVFIDHLGIPSLNIGFS
                     SRGGEGVYHSIYDSFEWYCRFGDPTFIYGRALAQVAGSLLLSLADDAVLPFEFGNLAD
                     TLEMYTVELQKLKTENVDLSPLRPAIAALRKAAARFDAARKSMKALSPARLRELNVMI
                     YQSEQAMLSSEGLPDRPWYRHEFYAPGVYTGYDAKTLPGIREAIEQKQWQRARTQVEI
                     VKKTIDAVTRQILAAADLMR"
     gene            2118..>3288
                     /locus_tag="VFA54_07055"
     CDS             2118..>3288
                     /locus_tag="VFA54_07055"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HZS50599.1"
                     /translation="MFRKLLLAFVLISGALSAGVARVEIQSRADVLEGRSFGLAGPYE
                     KLIGKVYFAVDPGNPINQIICDIDKAPRNSEGKVEFSSDLYILRPKQPERGNGALLFE
                     VSNRGSKGMLGMFNRARGSLDPTAAEEFGDGFLMRQGYTLVWLGWQFDMPMHPGLVRL
                     YAPKALGVKGLVRAEFIPTIKTSSHTLADRDHLPYLAADPNDPSAKLFVRDKVESPRT
                     TIPRSQWKFSADRGHVEMAAGFEPHKIYEVVYTSQDPAVIGLGPAGIRDFLSYVKYDA
                     ASPIKGIHHVYGFGVSQSGRFLRTFLYYGFNQDEAGRQVFDGVLAHVAGAGRGSFNIR
                     FGQASRDAHPFLNIFYPTDIFPFTDLEETDPETGLTDGLLVRAEKTHTAPKIFYTN"
BASE COUNT          646 a         1002 c          990 g          650 t
ORIGIN      
        1 ttttcactgc tagaatttac gatcatgggc cgccgttgcc gtcgctgttt agccactggt
       61 ctggttgctg ttagcctggc gtgggctgcc gaatccgatt ggcagcaaag atttcgcggc
      121 ctcccgcagc ccgatcacat ccgggaatac atacgggcca tgaccgaaga gccgcaccat
      181 gccggctcgc cggggtcgaa gaaggtcgcc gagtatgtgc tttcgaaatt ccgggagtgg
      241 ggcctggaag cgcagatcga ggaattcgaa gcccttttgc ctacgccgaa cgatcggacg
      301 ctcgagctga tcgagcctca gcaattcacc gcccgcctca aggagcccgc cattcccgaa
      361 gacaaggact cctcggatcc tggccagcta ccgactttca atgcctactc gcccgacggc
      421 gatgtcaccg ggcaagtcgt ctacgtcaat catggcgtgg ttgaagatta cgaacggctc
      481 gcccgcatgg gcgtcgatgt caaaggcaag atcgtgctgg cgcgctacgg cgccggctgg
      541 cgaggcgtca agccgaagct ggcctgggaa catggagcca tcggctgcct gatctactcc
      601 gacccccgcg aggacggata ctttcagggg gatgtttatc cgggaggccc cttccgtccg
      661 gcggaaggcg tacagcgcgg aagcgtcatc gacatgccca tttatccggg cgatccgctc
      721 actcccggat gggcttcgga aagggggggc cgcaagctgc cgctggcgga agcgcggacc
      781 ctgccgaaaa ttccggtttt tcccatctcc tatgctgacg ccacgcccat tttgaggaat
      841 atggccggac cggtggcgcc cgagccctgg cgtggggctt tacctctgac ttaccacgtg
      901 ggaccgggcc ccgccaccgt ccatctcaag ctctcttttg actggcaaac ccgtcccatt
      961 tacaacgtga tcgcccgcat tccgggctcc gattttcccg accaatgggt gatttacggc
     1021 aaccaccacg atgcttgggt aaacggagct cgcgacccgg ccagcggcaa cgccgcccta
     1081 cttgagaccg cccgcggctt gagcgagctt ctcaaacagg gctggaagcc gcggcggacg
     1141 attattctgg ccagttggga cgccgaggaa tggggcttga tcggctcaac ggagtggggt
     1201 gaaaagcatg ccgaggaatt gcggcagaaa gcagtggttt acatcaacag cgacggcaac
     1261 tcccgagggc tgctgcaagc ctccggctcg cactcgctcg aacacctgct gaacgaagtc
     1321 gctcgaagtg tgccggaccc ccaaaccgga aagccgctgt gggaagcgat gcgttcccgg
     1381 cggctccagg aggcttcgga tagcgaaaaa aagaaagcca tcgccagccg ctctgatctt
     1441 ggcatcgggg cgttaggcgc gggatccgac tacgttgttt tcatcgacca tcttggcatt
     1501 ccgtctttga acatcggctt ctccagccgc ggcggggaag gcgtctatca ctcgatttac
     1561 gattccttcg aatggtattg cagatttggc gatcccacgt tcatctacgg ccgggccctc
     1621 gcccaggtcg cggggtcgct ccttctcagc ctcgcggacg acgccgtgct gccgttcgaa
     1681 ttcgggaatc tggccgacac cctggagatg tatacagtcg agttgcaaaa gcttaagacg
     1741 gaaaatgtgg atctttcgcc gctccgcccg gcgatcgccg ccctgcggaa ggcggcggcg
     1801 cgatttgacg cggcgcgcaa gagcatgaag gcgctttcac cggcgcgttt acgcgagttg
     1861 aatgttatga tctaccagtc tgagcaggcc atgctgagct cagaaggcct gccggaccgg
     1921 ccatggtatc ggcacgagtt ttatgcgccg ggcgtctata ccggctatga cgccaagacc
     1981 cttccgggga ttcgggaggc catcgaacag aaacaatggc agcgggcccg aacgcaggtg
     2041 gagattgtaa aaaagacgat tgacgccgtt acgcgccaga tactggcggc cgcggatttg
     2101 atgcgctgag gtgatgtatg tttcgaaagc tgttgctcgc ctttgttctg atttccggag
     2161 ccctttcggc cggcgtggcg cgcgtggaaa ttcaatcgcg cgccgatgtg ctcgaaggcc
     2221 gctctttcgg gctggccggg ccgtatgaga agctgatcgg aaaggtctat ttcgccgtcg
     2281 atcccggtaa cccgatcaac caaatcatct gcgacatcga caaggctccg cgcaactccg
     2341 aaggcaaggt cgaattttcg tccgacctgt atatcctgcg gccgaagcag cccgagcgcg
     2401 gcaacggcgc actgctcttc gaagtttcca accgcggcag caaagggatg ttggggatgt
     2461 tcaaccgagc gcggggcagc ctcgatccca ccgcggcgga agagttcggc gacggcttcc
     2521 tcatgcgcca gggctacacg ctggtgtggc tcggctggca attcgacatg ccgatgcatc
     2581 ccggcctggt gcggctctac gcgccgaagg ccctaggcgt caagggattg gtgagggcgg
     2641 aattcattcc cacgatcaag accagctcac acacgctcgc cgatcgcgac caccttcctt
     2701 atcttgccgc cgatcccaac gacccgagcg ccaagctgtt tgtgcgcgac aaagtcgaat
     2761 cgccacgaac aaccattccg cgcagccaat ggaaattctc cgcggaccga ggccatgtgg
     2821 agatggccgc aggcttcgag ccgcacaaga tctatgaggt tgtatacact tcgcaagacc
     2881 cggctgtgat cggcctgggg cccgccggga ttcgcgattt tctctcctac gtgaagtatg
     2941 acgctgcctc tccgatcaag ggcatccatc atgtctatgg tttcggagtt tcgcaaagcg
     3001 gccgtttcct gcggacgttc ctttactacg gattcaatca ggatgaggcg gggcgccagg
     3061 tgttcgacgg agttctggcc cacgtggcgg gcgccggccg cggcagcttc aacattcgtt
     3121 tcggccaggc ctcgcgtgac gcccacccgt ttctcaacat cttttacccg accgacatct
     3181 ttcccttcac ggacctggaa gaaacagatc cggaaaccgg gctgaccgat gggcttctgg
     3241 tgcgggcgga aaagactcac actgcgccga aaattttcta caccaact
//
LOCUS       DASREK010000140         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_566505, whole genome shotgun sequence.
ACCESSION   DASREK010000140 DASREK010000000
VERSION     DASREK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566505"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VFA54_07060"
     CDS             complement(<1..397)
                     /locus_tag="VFA54_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50600.1"
                     /translation="MRFFVLLVIVSTPVWAQASGTPPEGAPAHSNGAKAAAHLSPEQE
                     QRILQEEVSQVANSPVDFIRAVERHLRKYPQSARRPELERAILRAAIDAHDDRRIILY
                     GEKVLEHSDDLMLLDRVARAFLSSDDAESA"
     gene            complement(394..1032)
                     /locus_tag="VFA54_07065"
     CDS             complement(394..1032)
                     /locus_tag="VFA54_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50601.1"
                     /translation="MRKAAGCFLILLAWAAGAQGSPKAGRIFFSKSFPGSIPEYFAIE
                     VEASGSATYRESPAEEDPLRFTLSASDTKEIFALAEKLQFFRKPLESKLKLANMGMKL
                     FRYEDGPVRNEVKFNFSEDPNARALADWFEKIGETERHRIALERAARFDRLGVNKALL
                     ALEISWDNHRIVAPEQLLPVLDKIATQPNFVHIAQARAASLAERIRTGKTAN"
     gene            complement(1029..1541)
                     /gene="ruvC"
                     /locus_tag="VFA54_07070"
     CDS             complement(1029..1541)
                     /gene="ruvC"
                     /locus_tag="VFA54_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZS50602.1"
                     /translation="MRVFGIDCGCERTGFGVVETDGRAHTFISAGVIETSAKDSMAQR
                     LSQIHDGLSRALRLYQPEVVAIEEVFHAANAKTALKLAHVRGIALLAAAQAGVPVGEY
                     SPLEVKSSVVGYGRAEKRQVQLMVASLLSLAEPAPEDASDALAVAICHGTNALAQARL
                     GWHQGAGKAS"
     gene            complement(1564..2310)
                     /locus_tag="VFA54_07075"
     CDS             complement(1564..2310)
                     /locus_tag="VFA54_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZS50603.1"
                     /translation="MSGHSKWATIKHKKAATDAKRGRLFTRLIKEITIAARSGGDVDS
                     NPRLRTAVAAAKAASMPADNIKRAILRGTGQLPGATIEEATFEGYGPGGVAVLVEVAT
                     DNRNRTVSEIRHLFGKYGGNLGENGCVSWMFSKRSLIVVPKEKVAEDQLMEIALSAGA
                     EDLRDDGDSWSILSAPESHEAVMEALKQNGIETTVSEITMVPQTHVKLEGKQAQAMLK
                     MSEALEDHEDVQNVYANFDIEEKEMEALAH"
     gene            complement(2436..>2657)
                     /locus_tag="VFA54_07080"
     CDS             complement(2436..>2657)
                     /locus_tag="VFA54_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50604.1"
                     /translation="AGVPAGQRLKILYLTQASVAPPTFVLFTDRQGKLHFSFERFLIN
                     QLRRQFDFEGTPLVIQVKARRKRGGSARP"
BASE COUNT          493 a          857 c          759 g          548 t
ORIGIN      
        1 gcgctgattc cgcatcgtcc gacgaaagaa aagcccgggc cacccggtcc agcagcatca
       61 aatcgtcgct atgctccagg accttttccc cgtacaagat gattcgccgg tcgtcatggg
      121 cgtcgatggc cgctctgaga atcgcccgct ccagctccgg ccgccgcgcc gattgcgggt
      181 atttgcgcag gtgccgctcg accgcccgga taaaatccac cgggctgttg gcgacctgcg
      241 aaacctcttc ctgcaagatc cgctgctcct gttccggtga aagatgggcg gccgccttcg
      301 cgccattcga atgggctggg gcgccctctg gaggcgtgcc gcttgcctgc gcccacaccg
      361 gggtcgaaac gattaccagt aggacgaaga atctcaattt gccgtcttcc cggtgcgaat
      421 ccgctcggcg agagaagccg cgcgggcctg tgcgatgtgg acaaagttcg gttgcgtggc
      481 gattttatcg agcactggaa gcaactgttc gggcgccaca atgcggtggt tatcccaaga
      541 gatctcgagc gccagcagcg ccttgttcac acccagacgg tcgaaccggg ccgcccgctc
      601 cagggcgatg cgatgccgtt cggtttcgcc gatcttttcg aaccagtcgg ccagggcgcg
      661 cgcattgggg tcctctgaaa agttgaattt cacttcattg cgcaccgggc cgtcttcata
      721 acggaaaagc ttcatcccca tgttggccag cttgagcttg ctttcgagcg gcttgcggaa
      781 aaattgcagc ttttcggcca acgcgaaaat ttctttcgtg tcgctggccg agagcgtgaa
      841 gcgcaacggg tcttcttcgg ccggcgactc gcggtaggtg gcgctgccgc tggcctcgac
      901 ttcaatggcg aaatactccg ggatgctgcc cggaaacgat ttggaaaaga aaatacggcc
      961 cgccttgggg gaaccctgcg cgcctgccgc ccacgccaga agtatgagaa agcagcctgc
     1021 cgcttttctc atgatgcttt tccagccccc tgatgccagc cgagcctcgc ctgcgccagc
     1081 gcattcgtgc cgtggcaaat ggcgacggcc agagcgtcgg atgcgtcctc cggagccggc
     1141 tcggcgagtg agagcaagga agccaccatc aactgaacct gccgcttctc ggcccggccg
     1201 tagcctacga cgctgctttt gacctcaagc ggcgagtatt ccccgaccgg gactcccgct
     1261 tgcgccgccg ccagcagcgc aattccgcga acatgtgcca gcttcagcgc cgtcttcgcg
     1321 ttcgccgcat gaaaaacctc ttcgatcgcg acaacttcgg gctggtaaag gcggagcgcc
     1381 cggctcagcc catcgtgaat ctgggaaagc ctctgcgcca tgctgtcttt ggcggaagtc
     1441 tcgatcacgc ccgcggaaat gaacgtgtgg gcgcggccgt cagtttccac tacgccgaaa
     1501 cctgttcttt cgcagccgca gtcgataccg aacactcgca tggcgttggc gccggcagcc
     1561 gggctaatga gccagcgctt ccatttcttt ttcctcgatg tcgaagttgg cgtagacgtt
     1621 ttgcacgtcc tcgtggtctt cgagagcctc gctcattttt agcatcgcct gcgcctgttt
     1681 gccctcaagc ttcacatggg tttgcgggac catggtgatt tcacttacgg tcgtctcgat
     1741 tccgttctgc ttcagcgctt ccataactgc ttcgtgcgac tcaggggccg acagaatcga
     1801 ccagctatcg ccatcatcgc ggaggtcttc ggcgccggcc gagagggcaa tctccatcag
     1861 ttgatcctcg gccaccttct ctttcggcac tacgatgagg gagcgcttcg aaaacatcca
     1921 cgagacacag ccgttctccc ccaggtttcc gccatatttg ccgaaaaggt gccggatctc
     1981 actgaccgtg cggttgcggt tgtccgtggc gacttccacc agaaccgcca cgcccccagg
     2041 accgtaaccc tcgaatgtgg cctcctcgat cgtcgctccc ggcaactgcc cagtgccccg
     2101 caagatggcg cgcttgatgt tgtcggcagg catactcgcg gctttggccg ccgccaccgc
     2161 agtgcgcagc cgcggattgg aatctacatc gccgcccgat cgcgcggcaa tggtgatttc
     2221 tttaatcaaa cgcgtaaaca gccggccccg cttggcgtct gtggctgctt tcttgtgctt
     2281 gattgtcgcc catttggaat ggcccgacat ggcacacctc acctactcca ataccagtag
     2341 caatataaaa agataccaaa aacccggggc tcgaagccag tgcgccaagc cagcctggtc
     2401 ccgtgccgga gtgctttcca gagcgtcgtt gtctttcacg gccgggcaga ccctccgcgc
     2461 ttgcgacgcg ccttcacctg gatcaccagc ggcgttccct caaaatcgaa ctgccgccgc
     2521 agttgattga tcaggaaccg ctcaaacgaa aagtggagct tgccttgccg gtcggtgaac
     2581 aagacgaaag tcgggggcgc gacagacgcc tgcgtcagat agagaatctt caagcgctgg
     2641 ccggcaggaa cccccgc
//
LOCUS       DASREK010000141         6619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_405077, whole genome shotgun sequence.
ACCESSION   DASREK010000141 DASREK010000000
VERSION     DASREK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405077"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFA54_07085"
     CDS             <1..710
                     /locus_tag="VFA54_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZS50605.1"
                     /translation="RSAKLKSGVLPPDHDIAVMRALRDAFGPEFGLRIDPNGMWSRGT
                     AIHVAKQLEECRPEYFEDPVWGLEGMARVKERTALPLATNMCVANFEHIAPAAAMNAV
                     DVILGDIYYWEGIRGVKALSTICECFRWGLSMHSGSELGVTLAAMIHTAAALPNLTYD
                     VDAHYHHLLDDVIAGGKMKYEGGSIAVPAGPGLGVDLDEDRMARYEELYGKKGDYYAR
                     FHEDTRRPDWFPVNPGW"
     gene            736..1488
                     /locus_tag="VFA54_07090"
     CDS             736..1488
                     /locus_tag="VFA54_07090"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZS50606.1"
                     /translation="MRLEGRAAIVTGAGGGIGRAIAKAFAREGAAVLVNDLPSAGSAA
                     ETAAQIADAGGKAVAYPADVKDLSAHEGLVAAALEHFGRLDILVNNAGIQFREPFLSA
                     TPEAWDATFAVNLKGPYFLSQKAAAAMIRFGGGRIINIASVHDTVPLRERSIYSITKG
                     GMKLLTRSLALELAEHGIHVNAVSPGAIHTNMNRQVFADPAHLARTLEKIPCGRIGVP
                     DDLAGAAVFLASDESSYITGATIYVDGGLLLH"
     gene            1504..2196
                     /locus_tag="VFA54_07095"
     CDS             1504..2196
                     /locus_tag="VFA54_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZS50607.1"
                     /translation="MSSPSETSAGMEQICERYLRLYAGLLYDVLEHLGYPNQVLSHHI
                     APLTPEMKLAGPAFTVKGAPACERDESRRYKRLKMITHMQHPCIEVRDCGTPFHVALY
                     GELSATTARAHGAVGAVVDGGTRDTTKLISMGFPVFAKYRTPIEAFGRWAMLDYQVPV
                     LLAGELTDTVQVNPGDFIFGDYDGVIVVPKDLTTQVLVECERVMGVEDNARVEFERGD
                     DPVAVFERHKRL"
     gene            complement(2285..3916)
                     /locus_tag="VFA54_07100"
     CDS             complement(2285..3916)
                     /locus_tag="VFA54_07100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZS50608.1"
                     /translation="MVDNIVRREAEVVRLLERTQPIAETYIQLLSPDAPNHVVDDVYF
                     IGKIDLQEGIDFDVFTPPPARAGFRSRFAAKTRFLPRGFAQMTLPDRDDFNQDTYEFH
                     YVRREFQGDLRCLVFDVLPRQGAGWGRFLGRIWVEDQKFYIVRFSGTYTSKSKKSPYF
                     HLNSWRVQTGTGAWAPAYIYVEETGGNFPAGRRPAEESFRGQVRIWGYKPNADSPREE
                     RTRILVEAPLARDESEGRDSYLVETRRNWEAQAEANIIERMERADLLAPAGGVDTVLQ
                     TVVNNLLVSNAAALDAPVRCRVLLTTPIESFTVGRTIVLSRGLIDSLPDEASLAMVLA
                     SELAHILLGHPTSTRYAFGDRILIEDSEIFQQFRFQRGPRERDEANRKALELLEHSPY
                     KPKIASAGLFLRQVEACAARMPNLFQARIGDPLAENGKVARMAELMESAPPLEPENPG
                     QVAALPLGSRVKVDPWTNRIALGQAARPRLAAGSEKTAFLLTPLMLPLSRRIVPATEA
                     SGSGSPGKADSRVGIPRTAGLPQHNQPAVDPQNAP"
     gene            complement(4038..4547)
                     /locus_tag="VFA54_07105"
     CDS             complement(4038..4547)
                     /locus_tag="VFA54_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZS50609.1"
                     /translation="MTAVPSHTPQPAQMGAFERNAPEQVSSFSLLKITGGSFGEDKSA
                     PKEAPPAGRRIVLESRFDAAEPPAEHPKTTWMSQPAITPVEILSKPKPVYTAEARLSG
                     VQGSVILDVLFTASGRVDVLRVVRGLGHGLDDAAIDAARKILFRPATAAGKPVDYRAT
                     VRIIFQLAF"
     gene            complement(5182..>6619)
                     /gene="lon"
                     /locus_tag="VFA54_07110"
     CDS             complement(5182..>6619)
                     /gene="lon"
                     /locus_tag="VFA54_07110"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZS50610.1"
                     /translation="LDADHYDLEKVKDRILDYLAVRQLNPTIKGPILCFVGPPGVGKT
                     SLGKSIARALERKFVRISLGGVHDEAEIRGHRRTYIGALPGQIIQGIRRAESNDPVFM
                     LDEIDKVGRDFRGDPASALLEALDPEQNNTFRDNYLDVPFDLSKVLFITTANMLDPIP
                     DALRDRMQVIELQGYTQTEKVRIAFNYLIPRQAEENGIKLGESIEFTEEGVTHIIRGY
                     TREAGVRNLEREIGTICRKQARRIAEGSLPAGMLTRVTPAVVEEMLGPPKVRPEHEIF
                     ERTRVPGVAVGLAWTPTGGDVLFVEAGILRGGSKGLIMTGHIGQVMQESMQAALSWVR
                     ANAEALHIDPEFMKNNDIHLHVPAGAIPKDGPSAGVTMVTALVSRLTGRRVRPRVAMT
                     GEITLSGLVLPVGGIKEKVLAAKAASIREVLLPAENEINVKEDLKSEQIGDMKLIYVH
                     RIEEVIEHALEPAEATAAASREEAAVPA"
BASE COUNT         1290 a         2022 c         1976 g         1331 t
ORIGIN      
        1 tccgttccgc caagctgaag agcggtgttc tgccgcccga ccacgatatc gccgtcatgc
       61 gagcgctgcg ggacgccttc ggcccggagt tcggcctgcg catcgatccc aacggcatgt
      121 ggtcccgcgg cacggctatc cacgtagcca agcagctcga agaatgccgg ccggaatatt
      181 tcgaggatcc cgtttggggc ttggaaggca tggcccgggt caaagagcgc accgcattgc
      241 cgctggccac caacatgtgc gtggcgaatt tcgagcatat cgctccggcg gccgccatga
      301 atgcagtgga cgtgattctc ggcgacatct actactggga gggaatccgc ggcgtcaaag
      361 cgctctcgac catttgcgag tgtttccggt gggggctttc catgcacagc ggcagtgaac
      421 tgggagtgac gctggccgcc atgatccaca ccgcggccgc gctgccgaat ctgacctacg
      481 acgtggacgc ccactatcac catctgctcg atgacgtgat cgcgggagga aaaatgaagt
      541 acgaaggcgg ctcgatcgct gttcccgccg gccccggcct cggcgtggat ctcgatgaag
      601 accgtatggc ccgctacgaa gagctctacg ggaaaaaagg cgattattac gcccggttcc
      661 acgaggacac ccgtcggccc gactggtttc ccgtcaatcc cgggtggtga cggcgatgcg
      721 caagcggaga tggcgatgag attggaaggc cgtgcggcga tcgtgacggg agcgggcggc
      781 ggcattggac gcgctatcgc aaaggccttc gcgcgagaag gcgcagccgt gctggtcaat
      841 gatcttccgt ccgccggctc cgctgcggaa acggcggcgc agattgccga cgccggcggc
      901 aaagcggtcg cttatccggc tgacgtgaag gatctgtctg cgcatgaagg gctcgttgcg
      961 gcggcgcttg aacatttcgg gcggctggat attctcgtta acaacgcggg catccagttt
     1021 cgagaacctt ttctcagcgc tacccccgaa gcctgggatg ccacattcgc cgtcaatttg
     1081 aaaggcccgt attttttgag ccagaaggcg gctgccgcca tgatccggtt tggcggcggc
     1141 agaatcatca acattgccag cgttcacgat acggtccctc tgcgcgaacg ctcgatttac
     1201 tcgatcacga aaggcggcat gaaactgctc acgcgttctc tcgccttgga attggccgag
     1261 cacggaatcc atgtgaacgc cgtttctccc ggggcgattc acaccaatat gaaccgccag
     1321 gttttcgccg atcccgctca tctcgcccgc accctcgaaa aaatcccttg cgggcgcatc
     1381 ggtgttcccg acgatctggc gggggcggct gtgtttttgg cgtcggatga gtccagctac
     1441 atcaccggcg ccaccatcta tgtagacggg ggattgctgc tgcactaatt caggaggtaa
     1501 tggatgtcgt ctccaagtga aacttcagcc ggtatggagc agatttgtga aagatacctg
     1561 cggctctacg cgggcctgct ctatgacgtt ctggagcacc tgggatatcc gaaccaggta
     1621 ttatcacatc atattgcgcc gcttacgccg gaaatgaagc tggccgggcc ggctttcacc
     1681 gtgaaaggag ctccggcctg cgagcgcgac gaatcgcgaa ggtacaagcg gctcaaaatg
     1741 atcacccaca tgcagcaccc ctgcatcgag gttcgcgatt gcggcacgcc gtttcacgtg
     1801 gctctctatg gagaactcag cgctaccaca gcccgcgccc atggggcggt gggcgcagta
     1861 gtggatggcg gcacgcgcga caccaccaag ctgatatcca tgggcttccc ggtattcgcc
     1921 aaatatcgaa ctcccattga agccttcggg cggtgggcga tgctcgacta tcaggtaccg
     1981 gtgctgctcg cgggtgaact caccgatacg gttcaggtga accccggcga tttcattttc
     2041 ggagattacg acggcgtcat cgtggttccc aaggacctca ccacccaagt cttggtcgag
     2101 tgcgaaaggg tcatgggcgt tgaagacaac gcccgcgtgg aatttgagcg cggcgacgac
     2161 cccgtggctg ttttcgagcg ccacaagcgg ttatagaacg ctctccgaat cggaagcgga
     2221 agcggaagag acgtggcagc gcggttatgg ctccggaaga cggatttcca ctccgcccga
     2281 gagttcaggg cgcattttgc ggatccacgg caggctggtt gtgctgcggc aggcccgccg
     2341 ttcgcgggat tcccactcgt gagtctgctt tccccgggga cccgctcccg ctcgcctccg
     2401 tcgcgggaac aatccggcgg ctgagcggca gcatcagcgg cgtcagcaga aaggccgttt
     2461 tttcgctgcc agccgccagc ctgggccggg cagcctgccc gagcgcgatg cgattggtcc
     2521 aaggatccac tttcacgcgc gacccgagcg gcagggccgc gacctgtccg gggttctccg
     2581 gctccagcgg cggcgcggat tccattagtt cggccatcct cgccactttg ccgttttccg
     2641 ccagcggatc tccaatgcgc gcctgaaaca gattgggcat gcgcgcggcg caggcttcca
     2701 cctgcctcag aaacagcccg gcgctcgcga tttttggctt ataaggggag tgctcgagca
     2761 gttccagagc cttgcggttg gcttcgtccc gttcccgcgg gccgcgctgg aagcgaaact
     2821 gttgaaagat ttcggagtcc tcaatgagaa tccggtctcc gaaggcatat ctcgtgctag
     2881 taggatgccc aagcagtatg tgtgccagct ccgaggccag caccatggcc aggctcgcct
     2941 catccgggag cgagtcaatc aagccccggc ttagaacaat cgtgcggccc acggtgaacg
     3001 attcaatcgg agtggtgagc agcacgcggc agcgaaccgg cgcatccaat gcagcggcat
     3061 tggagacgag caaattattc actacagttt ggagcaccgt atccacgccg ccggccggcg
     3121 ctagaagatc agcgcgctcc atccgctcga tgatattggc ttcggcttgc gcctcccagt
     3181 ttcgccgggt ttccaccaga taggaatcgc ggccctccga ttcatcccga gccagaggag
     3241 cctccacgag aattctggtt ctttcctccc ttggagaatc cgcattcggc ttgtatcccc
     3301 agattcggac ctgcccgcgg aagctttcct ccgccggcct ccggccggcc ggaaagttcc
     3361 cacctgtttc ttcgacatag atgtatgccg gcgcccaggc ccccgtgccg gtctgcacgc
     3421 gccagctatt taggtggaag tacggactct ttttcgattt cgaggtgtac gtaccgctga
     3481 aacgcacgat gtagaatttc tgatcctcga cccagatgcg gcccagaaag cgtccccatc
     3541 cagcgccttg gcgcggcaaa acatcgaata ccaagcaacg caaatcccct tgaaattccc
     3601 gccggacata atgaaattca taggtgtctt gattaaagtc gtccctgtcg ggcagcgtca
     3661 tctgggcaaa gccccggggc agaaagcgcg tcttggcggc gaaccgagat cggaagccag
     3721 cccgggctgg cggcggcgta aacacgtcga aatcaattcc ttcttgcaga tcgatcttgc
     3781 cgatgaagta aacgtcatcg acaacgtgat tcggggcgtc ggggcttaga agctggatgt
     3841 atgtttcggc gatcggctgc gtccgttcaa gcagccggac gacctcggct tcccgccgca
     3901 ctatattgtc aaccactcga tccaccggca ggccagcgcc ttgccctggt ccgcttcggc
     3961 cggcagacag ccaggcaatc ccaaaaaccg gaatcagaat gcgtaaatag cgggttataa
     4021 gcaaggcgga aatctcgtta aaacgccaac tgaaaaatga ttcgtacggt cgcgcgataa
     4081 tccactggct tgccggcggc ggtggctggc cggaacaaga tcttgcgggc cgcatcgatc
     4141 gcggcatcat ccaagccatg tcccagcccg cgaacgactc gcagcacgtc tacccgtccc
     4201 gatgcggtga agagcacatc caaaattaca gatccttgga cgcctgacaa gcgcgcctca
     4261 gccgtgtaaa cgggctttgg cttcgaaaga atttcgaccg gcgttatggc cggctgagac
     4321 atccaggttg tctttgggtg ttccgcaggc ggctccgcgg cgtcaaatcg gctctccagg
     4381 acgatgcgcc gcccggccgg cggagcttcc ttcggggcgc ttttatcttc tccgaagctg
     4441 ccgccggtga ttttcaacag agaaaaggaa gatacttgct ccggggcgtt ccgctcaaaa
     4501 gctcccattt gcgcgggctg tggcgtatgg gagggaactg cggtcacacc ctgcggcgca
     4561 tcaaacgtcc ctaagctcag gttatttttt tccggggcaa aggcgcttgg acgtacaggt
     4621 gcggagaaaa cgtcttcgtg gttcgaaaga gggcgattcg cgctgtccgg caatcctgga
     4681 gcctcgatcg ggattgcggg cgctccggca gggggcccgg cgacgtcccc caactcgacc
     4741 ccggagggcg gccttccagt aagcggcttg gaggacactc tcggcggcca agccggtccg
     4801 ttcgcgagcc ataacggcgc ccggcgcaat ggcgctcgtg gtagaaccaa cggcttgagg
     4861 ttagccgggg cgttgagaat cactgggacg acggcccgtt tccattcccc ctccacccgc
     4921 ggcgcgggaa aatggacaag cgttatggcg gcccagatgc cgaaggcatg caggcaggcg
     4981 ctcgctacca gcgaacacgg ggactccctg cccgtgcgaa tcccgcaaga aaatatcggc
     5041 gaaccgggct cgaaatcgcg gcatgggaag cccacgaagt gcttatcgtc aggtgcgaat
     5101 cagagcttga ggcaagcctt gaattccctg tttccagggc caaagatcgg ggctcagttt
     5161 gggcggccgg ccacgccgga gtcacgccgg cacggctgct tcctcgcggc ttgccgccgc
     5221 tgtcgcttcg gcgggctcca gagcgtgctc gataacctct tcgatgcgat gaacgtagat
     5281 cagcttcata tcgccgatct gctccgattt caagtcttcc ttcacgttga tttcgttttc
     5341 cgccggcagc aggacttcgc gaatcgaagc ggctttggcg gcaaggacct tttctttgat
     5401 gccgccgacg ggcagaacca gtccgctcaa ggtaatttcc ccggtcatgg ccactcgggg
     5461 ccgcacccgc cgcccggtca gccgcgatac cagggcggtg accatcgtga cgcccgcgga
     5521 aggtccgtcc ttcgggattg ccccggcagg gacgtggaga tggatgtcgt tgttcttcat
     5581 gaactctgga tcaatgtgca aagcttccgc gttggctctc acccagctca gagcggcctg
     5641 catcgattcc tgcatgacct ggccgatgtg tccggtcatg attaggcctt tcgatccgcc
     5701 cctcaaaatt cccgcctcga caaaaagaac atcgcctccg gtcggcgtcc atgccagccc
     5761 gacggctact ccgggaacgc gcgtgcgttc gaaaatctcg tgttccgggc gcactttcgg
     5821 cggtcccagc atttcctcca cgacggccgg cgtcactctg gtgagcatgc cggcgggcag
     5881 cgagccttca gcaatccgcc gggcctgctt cctgcaaatc gtgcctatct cccgttccag
     5941 gtttcgcacc ccggcctcgc gcgtataacc gcgaataatg tgggtaacac cctcttcggt
     6001 gaactcgatg ctttcaccca gcttgattcc gttctcctcc gcctggcggg gaatcaggta
     6061 gttaaaagcg atccggacct tttcggtctg cgtataacct tgcagctcga tcacctgcat
     6121 gcggtctcgc agggcgtccg gaatcggatc cagcatgttg gccgtggtaa taaacagaac
     6181 cttggaaaga tcgaacggca catcgagata attgtcccga aatgtgttgt tctgctcggg
     6241 atcgagcgcc tccaggagcg cggaagccgg gtccccgcga aaatctcgtc ctactttgtc
     6301 gatttcatcc agcatgaaga ccggatcatt gctttcagcg cggcgaatac cttggataat
     6361 ctgtcccggc agcgctccaa tgtaagttcg gcgatggcct cgaatttccg cctcgtcatg
     6421 cacgccgccc agtgatatgc gtacgaattt tcgttcgagc gcccgcgcaa tggatttgcc
     6481 caaagaagtt ttgcccactc cgggaggtcc gacgaaacag agaatcgggc ctttgatggt
     6541 tggattcaac tggcgtacag ccagataatc caaaatgcgg tcctttacct tctcgaggtc
     6601 ataatgatcg gcgtcgagg
//
LOCUS       DASREK010000142         5971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1230031, whole genome shotgun sequence.
ACCESSION   DASREK010000142 DASREK010000000
VERSION     DASREK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230031"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1337)
                     /locus_tag="VFA54_07115"
     CDS             complement(201..1337)
                     /locus_tag="VFA54_07115"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZS50611.1"
                     /translation="MIQPILPGAALGVLGSGQLGRMFAIAARRMGYRVHTLSPDEDTP
                     TGQIADLEVQASYDDLDAVSQFAAGVSVVTFEFENVPAATAEAAARCAPVRPSGSILH
                     ITQQRIREKSFLTQAGLPVTPYREVRSLDGLNRALADLGAPAVLKTAAFGYDGKGQFR
                     IDAPNRAAEAWDAIGKQQAVLEAFIDFECEISVVAARGEDGAFVHYGAIENQHSRHIL
                     DISVAPARVPARVAEEAVELARCVLDKLGVVGVLCVEFFVTHDGSLLINELAPRPHNS
                     GHLTVDACVTSQFEQQLRAVCGLPLGSTEMHRPAAMANLLGDLWTSGEPDWRAACAFA
                     DVKLHLYGKLDARPGRKMGHLTALNRDPDEAYRTVLAARESLRH"
     gene            complement(1334..1816)
                     /gene="purE"
                     /locus_tag="VFA54_07120"
     CDS             complement(1334..1816)
                     /gene="purE"
                     /locus_tag="VFA54_07120"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZS50612.1"
                     /translation="MQAAVGIIMGSKSDWETMRSAAETLTQFGVPYEAKVVSAHRTPA
                     WMVEYASGAEARGLQVIIAGAGGAAHLPGMVAANTLLPVLGVPVESHALKGMDSLLSI
                     VQMPGGIPVGTLAIGKAGAQNAALLAIAILANHDTALREKLRAFRTEQAAKVRTEELS
                     "
     gene            complement(1870..4206)
                     /locus_tag="VFA54_07125"
     CDS             complement(1870..4206)
                     /locus_tag="VFA54_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50613.1"
                     /translation="MNPQRCYFGELKRRSTAFLYLAFVLILSCLSLACKSTQQYINRG
                     NQLYAAGQYADAAINYRNALKKAPNSGEAYYRLGLALLKQNQVPDAYQAFNHAVTLDP
                     KNIPAQVQLATLSLAGYTRDPRHPAVLYKQAESAAEKLLAPGGDRKEGLRIRGALDLI
                     DNKPDKAVADLREAARIAPNDAEVGASLAQALIRNNQAEEGEKVARQVIQQHPTFPPA
                     YDVLYALYGIQQKPDQMEALLKSWSASDPKASTPILRLASLYYSRKQPEESEKLLKSM
                     LDRRDRFPQADLLVGDFHSLARDYQQALADYQRGESRDQVRQRVYEERVASTLQALGR
                     RQEAIKAADAILAKDPKSQFARVLKVEVLQQMGSQENMAMAATLARDLAKESPTNGRL
                     QLLAGQVLTAKGDLNEAYEDLGRAAREEPQSEVVQLALARLETLRKNYPAVLQHADAA
                     LAIRRDDPAARLFRVMGLTGTHAYVEAKTEAEQLARDTKDAPQVEMQLAVIALGQKRY
                     AEAEELFRKLYKPDSQNLEPLAGLVNTYEAENMPDRALALMKEEVQKSPESPGKAALL
                     VATEEASGKTADAIAELQKMAVKSPSSADIQFKLGVIQQKSGHMPEALQAFERARQLA
                     PDRKGIDAIVANLQEQLGKKTEAIASYRKALQKSPDDPAVLNNLAFLLADTGGDPKEA
                     MQLVSTAIRKAPDLPQLRDTMAWVHIKQHDAAGAIPILQALTTKYPDDPTYRYHYAVA
                     LSDSGNREAAKQQAEKALSNQPPLDVAVELRGLIAQAK"
     gene            4484..4639
                     /locus_tag="VFA54_07130"
     CDS             4484..4639
                     /locus_tag="VFA54_07130"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HZS50614.1"
                     /translation="MLLLLILLIILLIGAAPAWPYSRGWGYYPSGVLGTILIIFIVLL
                     LLGQVNL"
     gene            4653..5336
                     /locus_tag="VFA54_07135"
     CDS             4653..5336
                     /locus_tag="VFA54_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZS50615.1"
                     /translation="MKFVAALLLLATPGVYAVEEEGHAQKRVQVSAEVLSEIFHAKDR
                     GIPEDLIGKAECVGIVPSLKRAGFIVGAKYGKGVVVCRTARGWSGPSTIRIEGGSFGF
                     QIGAGETDLIFIVMNHHGVEDLMKDKFTLGGDASVMAGPLGRTGTAQTDALMRAEILS
                     YSRSHGVFAGVSLEGATLRPDHSDNREIYGRDVTQREILSGQVRRPAVARGIYSELDR
                     YTRSKRSAG"
     gene            5402..5584
                     /locus_tag="VFA54_07140"
     CDS             5402..5584
                     /locus_tag="VFA54_07140"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZS50616.1"
                     /translation="MTKDPVCGMQVDPNKAPATSNYQGKQYAFCGQECKDKFDKNPQQ
                     YTHAAQREPQEEPAHR"
     gene            5655..>5971
                     /locus_tag="VFA54_07145"
     CDS             5655..>5971
                     /locus_tag="VFA54_07145"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HZS50617.1"
                     /translation="MPYQPIEDYGLIGNLRTAALVGKNGSIDWFCFPHFDSPSVFGAI
                     LDEHKGGYFRISPTSAEITRKQLYWADTNVLVTRFLSADGVGEIVDFMPIGESKNDPP
                     YYGL"
BASE COUNT         1111 a         1791 c         1797 g         1272 t
ORIGIN      
        1 tcgcgaagct ggtccggcgt gagatatgcc gctcccagcg atccgagtcc gcccgcattg
       61 gatacgcccg cgacgagctc gggcgtggac ggtccgccgc ccatggggcc ttggataatt
      121 ggatactcga ttccgaggag cttggggagt tccatagcga tcactacaga atagtatcgc
      181 gcgcttcggc tggagtagtt ttaatgtctg aggctttccc gcgcggccaa tacagtccgg
      241 taggcttcgt cgggatcgcg gttcagcgcg gttaagtgcc ccatcttacg gccgggacgc
      301 gcatccagtt tgccgtacag gtgcagcttg acgtcggcaa aggcgcaggc ggcgcgccag
      361 tccggttcgc cggaggtcca cagatcgccc aacagattgg ccatcgcggc ggggcgatgc
      421 atttcggtgg atcccagggg cagtccgcac accgcgcgca actgctgctc gaactggctg
      481 gtgacgcagg catcgacggt taggtggccc gagttgtgcg ggcgcggcgc aagctcattg
      541 atcaacagag aaccgtcgtg agtgacgaaa aattcgacgc acaatacgcc caccacgccc
      601 agcttgtcga ggacgcagcg cgccaattcc accgcctcct cggccacgcg cgcaggaacg
      661 cgcgccggag caacggaaat gtccaagata tgccggctgt gctgattttc gatggcgcca
      721 taatgcacga acgcaccgtc ctcgccgcgc gcggctacca cggaaatctc gcactcgaaa
      781 tcgatgaagg cttcgagcac tgcctgctgc ttgccgatag catcccaggc ttcggccgcc
      841 cggttcgggg catcgatgcg gaactgtccc ttaccgtcgt aaccgaacgc cgccgtcttt
      901 aggaccgccg gagcccccag gtccgccagc gcgcgattta ggccgtcgag agaacgtacc
      961 tcgcgatacg gcgtgaccgg taagccggcc tgagtcaaaa aagacttctc gcggatgcgc
     1021 tgctgcgtga tgtgcaggat ggatccgctg gggcggacgg gcgcgcagcg agcagcagcc
     1081 tcggccgtgg cggccggaac attctcgaat tcaaatgtta cgacgctgac accggccgcg
     1141 aattgggaaa ccgcgtccag atcgtcataa gaagcctgca cttccaagtc cgcgatttgc
     1201 ccggtgggcg tgtcctcatc gggcgacagg gtgtggacac ggtaacccat gcgccgggcc
     1261 gcgatggcga acatacggcc caactggccg cttcccagaa cgccgagcgc cgcgccgggc
     1321 aatatcggct ggatcacgat aattcttccg tccggacctt ggcggcttgt tccgtccgaa
     1381 atgcgcgcag cttttcgcgc aatgccgtat cgtgattcgc caggatcgcg atcgccagca
     1441 gcgcggcatt ttgcgcgcct gcttttccga tcgcgagcgt ccccactgga atgccgcccg
     1501 gcatctggac gatggagagt agcgaatcca tccctttgag cgcatggctc tccaccggca
     1561 cgcctaagac cggcaacaac gtgttcgccg ccaccattcc gggaagatgc gcagcgccgc
     1621 ccgcgcccgc gatgattacc tgcaagccgc gcgcttcggc gccggatgcg tactccacca
     1681 tccaagccgg cgtgcgatgc gccgagacca ccttggcttc gtacggcacg ccaaattgcg
     1741 taagggtttc cgcggcggag cgcatcgtct cccagtcgga cttgctgccc ataatgatgc
     1801 cgaccgccgc ctgcattgag aactcactaa aatagcacgc gggatgggaa cgcgcgccgg
     1861 gcgcggtggt tattttgcct gagcgatgag gccgcgcaac tctacggcaa cgtcgagcgg
     1921 cggctggttg gacaatgcct tctccgcctg ttgtttggcc gcttcgcgat ttccgctgtc
     1981 gctcaacgcc actgcgtagt gatagcgata ggtaggatca tcggggtact tggttgtcaa
     2041 ggcttgcaaa atcggaatcg cgccggccgc gtcgtgctgc ttgatatgca cccaagccat
     2101 ggtgtcgcga agctgcggca gatcgggcgc tttgcggatc gccgtgctga cgagctgcat
     2161 cgcttctttc ggatcgccgc ctgtgtccgc caaaagaaat gccaggttgt tcagcactgc
     2221 cggatcgtcc ggcgattttt gcagcgcttt gcgatagctt gcaatcgcct ccgtcttttt
     2281 ccccaattgc tcctggaggt tcgccacgat ggcatcaatg cccttgcgat ccggcgcaag
     2341 ttgccgggcg cgctcgaacg cctggagcgc ctccggcata tggccgcttt tctgttggat
     2401 gacgcctagt ttgaactgaa tgtccgcgga ggatggactc ttgacggcca ttttctgcaa
     2461 ttcggcaatc gcatcggcgg ttttgccgct ggcctcttct gtggctacca gcagcgcggc
     2521 ctttccaggc gattcgggcg atttctgcac ttcttctttc atcaacgcca gcgcgcgatc
     2581 cggcatgttc tccgcctcgt acgtgttcac caggcctgcc agcggttcca aattctgcga
     2641 atccggttta tacagcttgc ggaacaattc ttcggcttcc gcataacgct tctggcccag
     2701 cgcgattacg gccagttgca tctccacctg cggcgcgtct ttggtgtcgc gggccagttg
     2761 ctcggcttcc gtcttcgcct ctacataggc atgcgtaccg gtcagcccca tgacgcgaaa
     2821 caggcgggcg gcgggatcgt cgcgacggat ggccagcgcg gcatcggcgt gctgcagcac
     2881 ggcaggataa ttcttccgca gcgtctccag gcgcgctaat gccagttgca ccacctccga
     2941 ctgcggttcc tcgcgtgctg cacggcccag atcctcgtag gcctcgttca aatcgccttt
     3001 ggcggtgaga acctgcccag ccaacagttg cagccttccg ttggtaggcg attctttcgc
     3061 caagtccctg gccagcgtgg ccgccatagc catgttctcc tggctgccca tctgctggag
     3121 cacttccact ttcaatacgc gggcaaattg gctcttggga tctttggcga gaatggcgtc
     3181 ggcggccttg attgcctcct gccggcggcc gagcgcttgc agcgtgctcg ccactctttc
     3241 ctcgtacacc cgctgccgaa cctgatccct cgattcgccg cgctggtagt ccgccagtgc
     3301 ctgctgataa tcacgcgcca ggctatggaa atcgcccacc agcagatccg cctgagggaa
     3361 gcggtcccgc cggtccagca tggatttcag cagcttttcg gattcctcag gctgcttgcg
     3421 cgagtagtag agcgacgcca ggcggagaat cggcgtggac gctttcggat cgctggcgga
     3481 ccagcttttg agcagcgcct ccatctggtc cggcttttgc tggataccgt acagggcgta
     3541 cagaacgtca taggccggcg gaaacgtggg atgttgctgg ataacctggc gcgccacctt
     3601 ctcgccttcc tctgcttggt tgttgcgtat cagcgcttgc gccagggacg cgccaacttc
     3661 ggcgtcgttg ggggcgatgc gcgccgcttc ccgcaaatcc gcgacggctt tgtctggctt
     3721 attgtcgatc agatccagag ccccccgaat gcgcagccct tccttccgat cgccgcccgg
     3781 cgccagcagt ttctcggcag ccgattccgc ctgcttgtac agaacggctg gatgcctcgg
     3841 atcgcgtgta tagcccgcca gcgaaagcgt tgcgagttgt acttgagcag gaatattttt
     3901 ggggtccaac gtcacggcat gattaaacgc ctgataagca tccggcacct ggttttgttt
     3961 cagcagcgcg agacccagcc ggtagtaagc ctctccggag ttgggtgcct tttttaatgc
     4021 gttgcgatag ttaatggcgg cgtcggcata ctgtccggcg gcatagagct ggttgccccg
     4081 attgatgtac tgttgcgtgc tcttgcacgc aagggacaaa caggacaaga tcaggacaaa
     4141 cgccaagtac aagaatgcag tagaacggcg cttcaactcc ccaaaatagc atcgctgtgg
     4201 gttcatgacg tctggttcat gagtctggac tctcaggatc actcaactaa gacaatcggc
     4261 tgagttgtcc ttgcgcgtta tcggcgagta acattttcag ttcgctttcg cggtcaagta
     4321 ggaagtgctt gtacatctgc tatcgcaagc ggccggctga gcccatttaa taagcccaac
     4381 aagcgccatc ctttcagtga gttatcggcc cggccagggt tttaatcagt ggttggaaca
     4441 tgaactgcct ttgttcggcc aagggaagga agggaccaac gatatgttac ttctgctaat
     4501 cctcttgatc atcctgttga tcggcgccgc gccggcttgg ccgtatagcc ggggttgggg
     4561 ttattatccg agcggtgttc taggaacgat tctcatcatc ttcatcgtgc ttttgcttct
     4621 cggacaagtg aatttataaa cgaaaggtaa ctatgaaatt tgtcgcagca ttgctgttgc
     4681 tcgcaacacc tggggtgtac gccgtggagg aggaaggcca tgcgcagaag cgcgtgcagg
     4741 tctccgcgga ggtattgagt gaaatttttc atgccaagga ccgcgggatt ccggaggacc
     4801 tgatcggcaa agccgagtgc gtcggcatcg tgccgagtct gaaacgcgcc ggttttattg
     4861 tgggcgccaa atacgggaag ggcgtcgtgg tgtgccgcac tgctcggggc tggagcggtc
     4921 cgtccaccat tcgaatcgaa ggcgggagtt ttggttttca gattggggct ggtgagacgg
     4981 acctgatttt catcgtgatg aatcaccacg gcgtggaaga cctgatgaag gacaaattca
     5041 cgctgggcgg cgatgccagc gtgatggcgg ggccgctggg ccgcacggga acggcgcaga
     5101 ccgatgcgct gatgcgcgcc gagattttgt cctattcgcg ttcgcacggc gttttcgcgg
     5161 gtgtgtcgct ggaaggcgcc actctgcggc ccgatcactc cgacaatcgg gagatttatg
     5221 ggcgcgatgt cacgcaacgc gagatcctga gcggccaagt gcgccgtccg gcagtcgccc
     5281 ggggaatcta ttccgaactg gatcgctaca cccgctccaa acgcagtgcc ggttgaacgg
     5341 ttacctgggg aaactcctgc ccttcgaggg cggtctcaca gatgagagcg aggtaacacc
     5401 aatgaccaaa gatccggttt gtggaatgca ggtggatccg aataaagccc cggcaaccag
     5461 caattatcag ggcaagcagt acgccttctg cgggcaagag tgcaaagata agttcgataa
     5521 gaacccgcag caatacaccc acgcggctca gcgcgagccg caggaggagc ctgctcacag
     5581 gtagcttctc tcacccgctc ccgagttgcg tagcgtgatg cattcgcgcg ggcactcggg
     5641 agcgtccctt attcatgccc tatcagccga ttgaagacta tggcctgatc ggaaacttgc
     5701 gtaccgcggc cctggttgga aaaaacggct ccatcgactg gttctgtttc ccgcattttg
     5761 attcgcccag cgtcttcggc gccatcctgg acgagcacaa aggcggctat ttccgtattt
     5821 cgcccaccag cgcggagatc acccgtaagc agctttactg ggccgatacg aacgtgctgg
     5881 taacgcgctt tttgtccgcc gatggggtgg gcgaaatcgt cgatttcatg cccatcggcg
     5941 aatcgaaaaa cgatccccct tactacggcc t
//
LOCUS       DASREK010000143         7398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_566844, whole genome shotgun sequence.
ACCESSION   DASREK010000143 DASREK010000000
VERSION     DASREK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566844"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..675
                     /locus_tag="VFA54_07150"
     CDS             163..675
                     /locus_tag="VFA54_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldoxime dehydratase family protein"
                     /protein_id="HZS50618.1"
                     /translation="MPAVKRITVDLSQFPDLVVIYLGMRVNTFKGLRRFLRLGPQIAK
                     SVAGRPDGLLLHENLFFSLFPLHAGMRQYWRDFESMETWARSLPHQSWWTEFLRDSGG
                     TGFWHETYFMRGGMEAVYDDMKIAPGLARFAPMRPARGPMFSARKRAGLPGEAKVTAP
                     VRESEYYRES"
     gene            complement(713..1642)
                     /locus_tag="VFA54_07155"
     CDS             complement(713..1642)
                     /locus_tag="VFA54_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZS50619.1"
                     /translation="MRICSLLPSATEMVFALGQEHLLAGVTHECDYPPQARQLPKITR
                     TNIDPALSSSQIEAAVSSTLAAAGSLYELDVSLLEQLQPDLILSQRLCDVCAVSAGRV
                     EQAAAALASHPRVLNLEPHSLQDIFENIRTVGQAIGCESRAAAVIADLERRVETIAAK
                     TKRCALRPRVFCMEWVQPPYCGGHWMKELVEIAGGRDDLAHLHRPSYRIEWRRVLDYA
                     PEIIVLTCCGFNLERNLREAETLAACEGFWSLPAAKSGRIFATDGSAYFSRPGPRIVD
                     SLEILAHLVHPEIFPAPLLAAAFAPAGLAPARK"
     gene            complement(1646..2167)
                     /locus_tag="VFA54_07160"
     CDS             complement(1646..2167)
                     /locus_tag="VFA54_07160"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HZS50620.1"
                     /translation="MSYLYVLAAVLLRLLPHPWNATPLAAMFLFSGATFRGKRESLLV
                     PLAALLISDYAAGQLLWHGRYPWFSATKCIAFLLIALIGWTLRDKMSFAKVAGASLAG
                     SVSFFLITNFGVWLAGSLYPKTAAGLAACYLAGLPFFRNTLLGDLAYAGVMFGSYYCI
                     AQRRWTPARQAAN"
     gene            2391..3479
                     /gene="aroB"
                     /locus_tag="VFA54_07165"
     CDS             2391..3479
                     /gene="aroB"
                     /locus_tag="VFA54_07165"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZS50621.1"
                     /translation="MRRIAVRTASHNYEALVERGALDKAAAHLRPFLRRKRLFVVTTA
                     PVWAQQGARLEQGLAGEPFIRLTMPDGEEHKRLATVEKLASQMLQSGADRGSLVAAFG
                     GGVVNDVAGFLAAIYMRGVSVIQIPTTLLAQVDAAIGGKTGVNLEGGKNLIGAFHQPE
                     LVLIDPDALATLPDREYRAGLYEVIKCAIIASRSLFQLLAGRREEVLRRSPAVVEELI
                     EESVRIKAGVVSTDEKEGDLRRILNFGHTLGHALEAETGYRRLLHGEAVAFGMRAAAW
                     LAARTGLLANGDYSSIEQLLDSYGPIPAVAGVGAESLLARLGADKKTIGGKVHFVLPE
                     GIGSARVVSDLDLALVREAAERALAAVA"
     gene            3476..4207
                     /locus_tag="VFA54_07170"
     CDS             3476..4207
                     /locus_tag="VFA54_07170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50622.1"
                     /translation="MIRGTTPAGVPVDDEIGAARYVRDMFARVAPRYDLANHLLSFQI
                     DRHWRRFTVEKVRSVLERPQARVLDLCCGTGDLLAALQRVHGRPVFGADFCRPMLAGA
                     AAKLGTRARLVEADALCLPLPDASFDLITVAFGLRNLVHYERGLREMHRLLRTHGWLA
                     VLEFSQPQGRFIAPLYGFYFHHLLPRLGGAVSGARHAYRYLQQSVDKFLTPDALSAAM
                     IGAGFRQVQYWPLTGGIAVLHLAVK"
     gene            4254..4910
                     /locus_tag="VFA54_07175"
     CDS             4254..4910
                     /locus_tag="VFA54_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50623.1"
                     /translation="MSWQRQTQRVLTAGLILSALYVTGVFIERALEPKRPRPRPESLP
                     LSADYYVALPKSYLRDFADAQALSGKALWVREGYRWLCQPGEALGPMEEVVVRRAFRE
                     GGQVWLEVARDQQPACSLPISSGGRFFLDDIFLIKDPHQVYKDWTQGVWKKIAEHRAE
                     PGMTEAQITFALGYGELARELSEPGGAHRVVDYIHGDRSARVTYVYGVAKQVEPLASR
                     "
     gene            5205..5429
                     /locus_tag="VFA54_07180"
     CDS             5205..5429
                     /locus_tag="VFA54_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50624.1"
                     /translation="MRPFALFVILAATCGVQQADWPTYGCDPGGTRYSSLRQINHDNV
                     KDQQYVVIAAGRHGKMRAKLGDYAVAFALS"
     gene            5558..7048
                     /locus_tag="VFA54_07185"
     CDS             5558..7048
                     /locus_tag="VFA54_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS50625.1"
                     /translation="MTDVAQTFRSHEILLTGGNGFLGKVILALLADRFPDLKRVHLLL
                     RPRRGQSPGERFETEILASPALHPLLRAGKRDFFEKKICVWAGDVGAPACGLTSQNLA
                     ELAGKIGVIINCAGLVEFYPPVNESFRSNVDGVRHVASLAKQLGAKLAHISTCFVCGE
                     SDGLVEETQPILGFYPRRQGPNDASFEHEREIRYCKDQVRRIQGSDSPASSGGGTRPS
                     RETERRLAELGRRRAEHWGWVNTYTYAKSLGEQIVAADGGLDFAIIRPAIIEGALRFP
                     FPGWIEGGRTSAPLVLMALSGMKHWPVRRDAPLEVVPVDLVAAAILAVTALLLEGRQE
                     RVYQLGTADVNPILLEPLVELLAGNVRFVSPEQARARRLRIQKQIAQVETLVAGIRRK
                     LETAGLPLNKPLAAWATALRARSLQAALREQTLDQYLPFILHNRYIFESENIRAAYSL
                     LSEKDRALLPWDPECIDWEDYWVNNQIKGIEKWIQPEAVKEWSFKI"
     gene            7162..>7398
                     /locus_tag="VFA54_07190"
     CDS             7162..>7398
                     /locus_tag="VFA54_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HZS50626.1"
                     /translation="MLVTPTTRIEPSLIEQQVLEIIRELLEELGARQAALSLRLDSSL
                     DRDLGLGSLERVELLVRCESRFDVRLPDEVAQEAE"
BASE COUNT         1371 a         2261 c         2345 g         1421 t
ORIGIN      
        1 tccctaaacg cccgcagggc cgcgtaaaga agacactttg ccggttctcg cctcgcagcg
       61 tggcgtttta cgcgaagatg cgataaattc tgtacatcgg cctgcggcgc ccgttgcaaa
      121 agagggtatt catgattctc aagtctggga ggagcccaag ctatgcccgc cgtcaaacgt
      181 atcacggttg acctttccca attcccggat ctggtggtga tctatctcgg catgcgggtg
      241 aacacgttca aaggactccg gagattttta cgcttagggc ctcagatcgc caaatcggtg
      301 gccggccggc ccgatggcct ccttctgcac gagaacctgt ttttttcgct gttcccgctg
      361 cacgcgggaa tgcggcagta ttggcgcgat tttgaatcca tggagacctg ggcgcgctcc
      421 ttgccgcatc agagctggtg gaccgaattc ttgcgtgatt ccggcggtac gggcttctgg
      481 catgagacct atttcatgcg cggcggcatg gaagccgtct atgacgatat gaagatcgca
      541 ccgggattgg cgcgatttgc ccctatgcgg ccagcccgcg gccccatgtt ctcagctcgc
      601 aaacgcgcgg gcctgccggg cgaggccaaa gtgaccgccc cggtgcgcga atccgagtac
      661 tatcgcgagt cgtaatggca agcgcttgcg cgcatagtcg cgcaagggga tctcattttc
      721 ttgcaggagc caagccggca ggagcgaatg ccgccgccag cagcggcgcc gggaaaatct
      781 ccgggtgaac caggtgggcc agaatctcca ggctgtctac aatgcggggg ccgggacggc
      841 tgaaataggc ggagccgtcg gtggcgaaaa tgcgcccgga tttcgccgcc ggcaggctcc
      901 aaaagccctc gcaggccgcc agcgtttccg cctcgcgcaa attgcgctcc agattgaaac
      961 cgcagcaggt caggacaatg atctccggcg cgtaatcaag cactctgcgc cattcgatcc
     1021 ggtacgaggg ccggtgcaga tgggcgagat cgtcgcgccc cccggcgatt tcgacaagtt
     1081 ctttcatcca atggccgccg cagtacgggg gctgtaccca ctccatacag aacactcgcg
     1141 gccggagcgc gcagcgtttc gttttggctg cgatggtctc aactcgccgt tccagatccg
     1201 caatcaccgc tgcggcccgg ctttcgcagc cgatagcctg gcccacagtg cggatgtttt
     1261 cgaagatgtc ttggagcgaa tgcggctcga ggttcagcac gcgcggatgc gatgcgaggg
     1321 cggctgcggc ttgctcgacg cggcccgcag ataccgcgca gacgtcgcag agccgctggc
     1381 tgagaatcaa gtcaggctgt aattgctcca gcagagaaac atccagctca taaagcgagc
     1441 cggccgcagc cagggtcgaa ctgacggcag cctcgatctg ggaactggag agcgccgggt
     1501 cgatattggt tcttgtgatc ttgggcaatt ggcgggcctg gggcgggtaa tcacactcgt
     1561 gggtgacgcc ggccagcaga tgctcctgcc ccagcgcaaa aaccatttcc gtagcgctcg
     1621 gcaatagcga gcagatgcgc acagctcagt tggcagcttg gcgggcggga gtccagcggc
     1681 gctgcgcgat gcagtaatag gagccgaaca tcacgccggc gtaagccaaa tctcccagca
     1741 gcgtattccg gaaaaacggc agcccagcaa gatagcaggc agccagtccg gcagccgtct
     1801 tcgggtagag gctcccagcc agccagactc cgaaattcgt gataaggaaa aagctcaccg
     1861 agccggccag cgaggcgccg gcgaccttcg caaagctcat cttgtcgcga agcgtccacc
     1921 cgatcagcgc gatcagcaga aacgcaatgc acttggtggc gctgaaccag ggataccggc
     1981 cgtgccagag aagttggccg gccgcgtaat cgctgatcag aagcgccgcc agagggacga
     2041 gcaggctctc gcgtttgccg cggaacgtag ctccgctgaa caggaacatg gcggcgagcg
     2101 gcgtggcgtt ccacgggtgc ggcaggaggc gtaacagaac ggcagccaga acgtaaagat
     2161 aagacatcgc gcgcaacctc tcggtcctta tgatagcaga ggcggcgctg cacgaccgat
     2221 agtggaaaac agtgttggaa agacacccca caaacggcgg cggccgcccc tgcgccgtat
     2281 gccgcccggc ccgggatcct ctctgaaggg cagccttgcg cttgcgctgc aaagccgccg
     2341 cggtccttcg cccgccgccg ggacgcggcc atccgcgtta acatcttggt atgcgccgca
     2401 tcgccgtgcg tacggcttct cacaattatg aagcgctggt ggagcgcggc gctctcgaca
     2461 aggccgcagc ccatctccgg ccttttctta ggcggaaaag gttattcgta gttaccacgg
     2521 ctccggtgtg ggcgcagcaa ggggcccgcc tcgagcaagg cctggcagga gagcctttta
     2581 tccgcttgac gatgcctgac ggcgaggagc acaagcggct cgccacggtt gaaaagcttg
     2641 cgtcccaaat gctccagtcc ggcgctgacc gaggcagtct ggtagccgcg ttcggcggcg
     2701 gcgtagtgaa cgacgtcgcc ggctttctgg cggccatcta tatgcgcggc gtcagcgtca
     2761 ttcagattcc cacgaccttg ctcgcacagg tggatgccgc gattggcggc aaaaccgggg
     2821 tgaatctcga gggcggcaag aatttgatcg gcgctttcca tcagccggaa ctggtgttga
     2881 tcgaccccga tgcgctggcc acgctgcctg atcgcgagta tcgcgccggc ttgtacgaag
     2941 tgatcaagtg cgccattatc gcgagccgga gcttattcca gctcctcgcg gggcggcgcg
     3001 aggaggtgct acggcggtca cccgcggtgg tggaggaatt gatcgaggaa tccgtgcgca
     3061 tcaaggcagg cgtggtctcg accgacgaaa aggaagggga tctgcgccgc atcctcaatt
     3121 tcgggcatac tctcggccat gctctcgaag cagaaaccgg ctaccggcgc cttctgcacg
     3181 gcgaggctgt cgccttcggc atgcgagcgg cggcctggct ggccgcccgg acggggctgc
     3241 tcgcaaatgg agactacagc tcgattgagc aactactgga cagctacggt ccgatcccgg
     3301 ctgtcgccgg ggttggcgcg gaaagccttc tggcccggct gggcgccgac aagaagacaa
     3361 tcgggggcaa ggtccacttc gttctccccg aggggatcgg ctccgcgcgc gtcgtttccg
     3421 acctcgattt ggcgctggta cgcgaggcgg ctgagcgcgc actggcggca gtggcatgat
     3481 tcgcggcact accccagcgg gcgttcccgt tgacgatgaa atcggcgccg cgcgctacgt
     3541 gcgcgacatg ttcgcccgcg tagccccgcg ctacgatctt gccaatcatc tgctttcctt
     3601 ccagatcgac cgtcactggc ggcggttcac agtggaaaaa gttcgcagcg tgctcgaacg
     3661 gccgcaagcc cgcgtgctcg atctgtgctg cggaaccggc gacttgctgg ccgccttaca
     3721 gcgcgttcat ggccgccccg ttttcggcgc tgatttctgc cggcccatgc tcgccggagc
     3781 ggcagccaag ctcgggaccc gcgcccgcct cgtcgaggcc gacgctttgt gtctcccgct
     3841 gcctgacgcc agcttcgacc tgattacggt tgccttcggg cttcgcaacc tcgtccatta
     3901 cgagcgggga ctgcgcgaaa tgcatcgcct gctccgcaca cacggatggc tggccgtttt
     3961 ggagttttcc caaccgcagg gccgattcat cgctccgctc tacggcttct actttcacca
     4021 ccttctgccg cgtctgggcg gagctgtctc tggagcgaga catgcctacc gctacctcca
     4081 gcagtccgtt gataaattcc tcacgcccga tgcgctgtcg gcggcaatga tcggcgccgg
     4141 cttccgccaa gtgcagtact ggccgctgac gggtggcatt gccgtgttgc atctcgcggt
     4201 caaatgattg gcgggccgga aatggaagga tacgtgcacg cttcctttgc ggtatgagct
     4261 ggcagcgcca aacccagcga gtgctgaccg cgggccttat cttgtcggct ctgtatgtca
     4321 ccggcgtttt tatcgagaga gcgctcgaac cgaaacggcc gcggccccgt cccgagagcc
     4381 ttcctctcag cgccgattac tacgtggcgc tgccgaaatc ctatttgcgc gatttcgccg
     4441 acgcgcaagc tttgtccggc aaagcgcttt gggtgcgaga aggctaccgc tggctttgcc
     4501 agcccggcga agcgcttgga ccgatggaag aagttgtggt tcggcgggcc ttccgggaag
     4561 gcggccaggt ctggctggag gtggcccgtg accaacagcc cgcctgctcg cttcccattt
     4621 cctcaggcgg ccggttcttc ctcgacgaca tcttccttat caaagatccg caccaggtct
     4681 ataaggactg gacccagggg gtttggaaga aaattgcgga gcatcgggcc gagcccggca
     4741 tgacggaggc acagattact ttcgcgctcg gctatgggga attggcccgc gagttgagcg
     4801 agccgggcgg ggcgcatcgc gtggtcgatt acatacatgg cgacaggagc gcccgggtca
     4861 catatgtcta tggcgtggcc aagcaggtcg aacccctggc atcaaggtaa tgacatcgaa
     4921 gcaattgccg gctcgctagg gcgctggttc ggaataaccc ctccgaaact tcgcaccgga
     4981 tcggcgaaaa gacgaccacg tcccgcgcat agcggcagtt atccacattc accttcgcgc
     5041 gcgcgagcgg ctccttgata gccccggagg atctggttag cttagttcct ttacagcacc
     5101 ttctcgcagg cggccgcggc ttgcccggcc cggccacacc cggttgctgg ggccgttcct
     5161 tgcccgcctt tgggcagtct ggtggtgcaa cactctgcac aactatgaga ccgtttgctc
     5221 tgtttgtgat cctggccgca acttgcggcg tgcagcaagc tgattggccc acctacgggt
     5281 gcgatcctgg cggaactcgc tactcctcgc tccgccagat caaccacgac aacgtgaaag
     5341 atcagcaata cgtcgtgata gctgccggca gacacggcaa gatgcgagcc aagctcggcg
     5401 attacgcagt cgcgttcgcg ctgtcgtagg ccgggctgcg aggctccgcg cctgctgcct
     5461 tgacactcgc ccggcgatct cgctaagtta gtttcttaac agcaccttat cgcgcctggc
     5521 cggctccggt tgccgaaaaa agggcgaggt tcttcctgtg acggatgtag ctcagacgtt
     5581 ccggagtcac gaaatcctgt tgaccggcgg caatggattt ctcggcaagg tgattctggc
     5641 gcttcttgcc gaccgcttcc ccgatctgaa gcgcgtccat cttctgctcc ggccgcgccg
     5701 cggccagtcg cctggtgagc ggttcgagac cgagattctc gcctcgccag ccctccatcc
     5761 tttgcttcgc gccgggaagc gcgacttttt cgagaaaaaa atctgtgttt gggccggcga
     5821 tgtcggcgca ccggcttgcg gtttgacttc gcaaaacctg gccgaactgg cgggcaaaat
     5881 cggcgtgatt atcaactgcg cgggtctggt cgagttctac ccgccggtga acgaatcttt
     5941 tcggtcgaat gtggacggcg tgcggcacgt cgccagcctg gccaagcagc ttggcgccaa
     6001 gctggcgcac atttccacct gttttgtttg cggcgaaagc gatggtttgg tggaggagac
     6061 gcagcccatc ctcggcttct acccgcgccg gcaaggccct aacgacgcca gcttcgagca
     6121 tgaacgggag attcgttatt gcaaggacca ggtgcggcgg attcagggat cggacagccc
     6181 cgcatccagc ggcggtggga cccggccatc gcgggagacc gagcgccggc tggcggagct
     6241 cggccggcgg cgcgccgagc attggggctg ggtgaacaca tatacttacg ccaaaagctt
     6301 gggcgagcag atcgtggccg cagacggcgg gctggacttc gcgatcattc gcccggcgat
     6361 catcgagggc gccctgcgtt tcccatttcc agggtggatt gagggcggcc gcaccagcgc
     6421 gccgctggtc ctgatggctc tgagcggaat gaaacactgg ccggtccgga gagacgctcc
     6481 cctcgaagtc gtgccggttg atctggtggc ggcagcgatt ctggccgtca cggccttgct
     6541 gctcgaggga cgtcaggagc gcgtgtatca gttaggcacg gctgacgtaa atcccatcct
     6601 cctggagccg cttgtcgagt tgctggctgg aaatgtccgg tttgtttcgc ccgagcaggc
     6661 cagggcgcgc cgcctgcgga tccaaaagca aattgcccag gtggaaacct tggtggcagg
     6721 cattcgcaga aagctcgaga ccgccggatt gcccttgaat aagcctctgg ccgcctgggc
     6781 taccgccttg cgcgcccgca gtttgcaggc ggcgcttcgg gagcagacac tcgaccagta
     6841 tttgccgttc attctgcaca accggtacat attcgagtct gaaaatattc gcgccgccta
     6901 ttcgctgctg tctgagaaag atcgagcgct gctcccttgg gatcccgagt gcatcgattg
     6961 ggaagattac tgggtcaaca atcaaatcaa gggcatcgaa aaatggatcc agcccgaggc
     7021 tgtgaaggag tggagtttca agatctgaga ggctgttgaa aaaaccggct ctggaactgc
     7081 ggggccatcc cagcccgcgc ttttgccggc agccataagg ctcgttgcct caagaaaccc
     7141 tgatgggact gcaagtgtcg aatgcttgtg acgccaacca cccggattga accttcgttg
     7201 attgagcagc aagtcctgga aatcatccgg gaacttctgg aagagctcgg ggcccgccag
     7261 gcagcattga gtctccggct cgactcgtcc ctggaccgcg atctcgggct gggcagcctc
     7321 gagcgagtgg agttgctcgt tcgctgcgaa tcgagatttg acgtgcggct gccggacgaa
     7381 gtggcgcagg aggcggaa
//
LOCUS       DASREK010000144         7625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1397247, whole genome shotgun sequence.
ACCESSION   DASREK010000144 DASREK010000000
VERSION     DASREK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397247"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFA54_07195"
     CDS             <1..618
                     /locus_tag="VFA54_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZS50627.1"
                     /translation="QQRLDDLAALLAKREQPEGMVLGIEGAPFLGDKDAKVTVIEFFD
                     FQCGFCARHAAKTFPLLLNEYIKTGKLKYVVRDFPLDTHPLAPKAAEAAHCAGEQGKY
                     WEMFDRLFENQKLLSPDSLTAHAEAIHLDAARFRQCLDSGRYESLVKKNQQAGELAGV
                     DGTPAFFFGLTEKGSPTIRATKSIGGAEPYPYFKDAIEVLLGSGK"
     gene            947..1852
                     /gene="ligD"
                     /locus_tag="VFA54_07200"
     CDS             947..1852
                     /gene="ligD"
                     /locus_tag="VFA54_07200"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZS50628.1"
                     /translation="MSRQTSETVRVGRQTVEITRPNKVLFPADGITKRELAEYYRQVA
                     PIILPHLRGRPLALERYPEGIGGPGFFQQKAGAYFPQWIRTASLPKQGGSVRHVICGN
                     RATLVYLAGQAVITPHAWLSRIDKPNNPDQMIFDLDPSDGDFKAVCGAAKKLRELLAE
                     YGLPAFVKTTGSRGLHVLVPLDRRADFDRIRVFARQIAQRLAQTDPEHLTTDARKTKR
                     RGRIFIDTARNSYAQTAAPPYAVRARDGAPVAVPLHWEELDNPQARADQFTIRNIWER
                     LHSIGDPWKSLKGPTRKALELPEVL"
     gene            2159..2620
                     /locus_tag="VFA54_07205"
     CDS             2159..2620
                     /locus_tag="VFA54_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50629.1"
                     /translation="MAVTLLIIGVCFGLFVYWFRYTVVLILRTPAGPEYAKRVAAANQ
                     LNFVQVRQKLHSSVDAGQLGFLCDALHRDYTSLKYLLRHAATLQANGYTAEQRLLMIN
                     FRCMALWCKMILRLRPAAAKLALLEMSGTLEYFANAMGRRFATLAGEASQA"
     gene            2715..3557
                     /locus_tag="VFA54_07210"
     CDS             2715..3557
                     /locus_tag="VFA54_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50630.1"
                     /translation="MSTYHFARFLVRAFAGISLGLFALSVGQAQQAPAKVQASATASN
                     PSIPVDEIIQKFAAKELEFKRARDNYTYRQTVRVLELDDDGAVRGRYEMVSDIIFSPS
                     GKRMERVVRAPMSTLKRISLDPGDEQDLRNVQPFVLTTEDIPKYNISYVGPQKVDELD
                     TYVFAVKPKKLEPGQRYFEGQIWVDQQDLQIVKTYGKGVGLQKKGSDHQYPRFETYRD
                     QIDGKYWFPVYTRADDVLHFQTGDQRVRMTVKYEDYKQFKSQVGIEYEGVVNDKKPED
                     EKKK"
     gene            complement(3633..4322)
                     /locus_tag="VFA54_07215"
     CDS             complement(3633..4322)
                     /locus_tag="VFA54_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50631.1"
                     /translation="MRTFLIAAFLFALLPAQTPEAPSLESVLAKMDAASVAFRNLTAH
                     IRSVKYTAIVNDQTVDEGTIWVMRVKPRVSRMLIEFTSPDKYYVSVTEKKAEIYRPKI
                     ATIEEYDVTKYATLRDQFWLLSFGTSGRDLAANYQVRMEKPESVVGQPAVKLELIPKS
                     AELLQHVPRIEMWVSTVDWQPVQQKFYDVTPGDYRLSTYTNIKLNDPKFQASRLEIHP
                     PRGVKRIQPQK"
     gene            complement(4325..4690)
                     /locus_tag="VFA54_07220"
     CDS             complement(4325..4690)
                     /locus_tag="VFA54_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50632.1"
                     /translation="MRKIWKLIRPAVFWQYRRGSWQYDVIVGLILVFIFATPRAWFRD
                     QPRIPNPQQIVMLPSENGRPVFWIDPELVADAPPEQLEAKIHALLQKRTGRQVGILKV
                     EPAKDSEGSLKGYLVRAQF"
     gene            4843..5844
                     /locus_tag="VFA54_07225"
     CDS             4843..5844
                     /locus_tag="VFA54_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZS50633.1"
                     /translation="MRGMVREARRFIRLASHIQLMPVLESGEEQLLVGGQAVIEGVMM
                     RAPHSYCVTVRRPDGSVAAEQAPLQRPSEKHRIFKYPVFRGLGTLGQAMALGIKALRF
                     SADTAIEKDPAQKDGKAGQISNGMITANVLFSVLFFLFLYKFVPLFLATQLKDRFAAL
                     DNYFLFNLIDGSVRISIFLAFLGVISRTKDIRRVFEYHGAEHKVVFNFESGQPVSVPN
                     AQCFSTLHPRCGTSFLLVVMVISMLIYALVPFQGFGLRLLSRVLLLPVIVGLSYEVIR
                     FAARRQGTLWASMVAPGLWLQKVTTRPPSDEQVEISILSLEGAMALEKQQGGELVIA"
     gene            5929..7008
                     /gene="prfA"
                     /locus_tag="VFA54_07230"
     CDS             5929..7008
                     /gene="prfA"
                     /locus_tag="VFA54_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZS50634.1"
                     /translation="MQYIAKLDQLEARFEELTQQLADPSVIADNARYRKSAKAHSDLA
                     EVVEKYRAWKAVQRNMEGTRPMLEEADPEIREMAQQELASLEQRAHDLEQELKILLLP
                     KDPNDEKSIVLEIRAGTGGDEATLFAAEIFRMYTRYAESQGWRVEVTSLSESSLGGMK
                     EVIALVSGEKVYSKMKYESGVHRVQRVPATEQQGRVHTSAVTVAVLPEADEVEVEIDP
                     KDIRIDTFCSSGPGGQSVNTTYSAVRITHLPTGLIVSCQDEKSQIKNRAKALRVLRSR
                     LYEMERQKQHEAMAEERRSMVGSGDRSEKIRTYNFPQNRVTDHRIGLTLYQLDQVMDG
                     KLDPLIDALVTHYQAEKLKQEAVLI"
     gene            7056..>7625
                     /locus_tag="VFA54_07235"
     CDS             7056..>7625
                     /locus_tag="VFA54_07235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HZS50635.1"
                     /translation="MIRLNVFTALRQGTEILEKENISAPRLTAEVLLAHALRCERVDL
                     YAHPERELTLAEQAHYGRCLHERLARKPVQYITGRQEFYGRMFSVSPAVLIPRPETEL
                     VVEAALRYAAAARRILDAGTGSGCLAVTLKKEWPHAATFASDISVAALRVAEENARRL
                     EADVHFFCGNLLDACRAQEMDLIVSNPPYV"
BASE COUNT         1562 a         2197 c         2319 g         1547 t
ORIGIN      
        1 cagcagcggc tggatgatct tgcggcgctg ctggccaaaa gggagcagcc cgaaggcatg
       61 gtcctcggca tcgagggcgc gccatttctc ggcgacaaag atgcgaaggt cactgtaatc
      121 gagttcttcg atttccagtg cggtttttgc gcgcgccacg cggccaagac gttccccttg
      181 ctgctgaacg agtacatcaa gaccggcaag ctgaagtacg ttgtccgcga cttccctttg
      241 gatacgcacc ccttggctcc caaagccgcc gaagcggcac actgcgccgg ggagcaaggc
      301 aaatactggg agatgttcga ccgccttttt gaaaaccaaa agcttctctc gcccgacagc
      361 ctgacggcgc atgccgaggc gattcatctg gatgcggcga ggtttcggca atgtttggat
      421 agcggccggt acgaaagcct ggtgaagaag aatcaacagg cgggagaact ggcaggggtg
      481 gacggcaccc ctgcgttctt tttcggcttg accgagaaag gcagtcccac gatccgcgcc
      541 accaagagca tcggcggtgc ggaaccttac ccttacttca aagacgccat cgaagttttg
      601 ctcggctccg gcaagtgacc gtccggccag gcggccggcg ccttccctgc gcggcgcgag
      661 atcagtctca atccggcgtt ggtctccgca aagcggtggg gaacggccgt tccagccgcc
      721 cgcttcggcc tcgcaacagg agttcatcgt ggaccgcggt ctaacgcccg tgtttgcctt
      781 acgggacttc ggctctcccg gaaatctcct tagccttcgt tcagttggca agtcgcgata
      841 ataaagccag gcgtacgcag cccctctggc cgtaagccgc ctgtaaagcg ccttcggagc
      901 gcgcgcaggc taggtcacgc gcgtcccgag gaccgaaagg atctccgtga gccgtcagac
      961 cagcgagacg gttcgagtag gacgccagac cgtagagatt acacgcccca acaaagtact
     1021 ttttccagcc gatgggatca ccaagcggga actggccgag tattatcgcc aagttgcgcc
     1081 gatcatactg ccgcatctgc gcggtcggcc cctcgctttg gagcgctatc ccgaggggat
     1141 tggcggtccg ggcttttttc aacaaaaggc gggtgcttac ttcccacaat ggattaggac
     1201 agcgtctctg ccgaaacaag gcgggtcggt ccgccacgtt atttgcggta acagagccac
     1261 gctggtatac ctcgctgggc aggcggtgat tacaccccac gcatggctaa gccgcatcga
     1321 taaaccgaac aacccggatc aaatgatttt cgatctcgat ccttcggatg gagacttcaa
     1381 ggccgtgtgc ggagcggcga agaagctccg cgaacttctg gcggagtacg ggctgccggc
     1441 gttcgtcaag accacaggtt cgcgggggtt gcacgtcctt gttccactgg atcgccgcgc
     1501 cgatttcgac cggatccggg tcttcgcccg gcagatcgcc cagcgcctgg cgcaaaccga
     1561 cccggaacat ttgacgacgg acgcgcgaaa aaccaagcgg cgaggacgaa ttttcatcga
     1621 taccgcccgc aactcctacg ctcagaccgc ggcgccgccg tacgcggtgc gggcgcgcga
     1681 cggcgctccg gtggcggttc cgctccattg ggaagaactg gataatccgc aggcgagggc
     1741 tgaccagttc accattcgaa atatttggga acggcttcac agcatcggcg acccctggaa
     1801 aagcctgaaa gggccgacgc gcaaagctct cgaactgccg gaggtattgt aaggggcctc
     1861 ccggaagcgg cggccgacca agcaggcgaa gattgccccg gcgactcgac gccgatcacc
     1921 cgtacgggta ctcgccaagc cttgggccta taagttacat tccttgcttg cccaggctca
     1981 ccctggcgtc gccggggcca gtccggaaat ggcggtttct ttgtctcttg aggctttgta
     2041 ttatcagtga gttagaaaat tgtgccgtac cctcttgtga ataaacgaac attgtggtac
     2101 gtttgctttt gtacccggta gttctgggaa acatccccga aggattaccg attccgccat
     2161 ggccgtgacg ttgctcatca ttggagtgtg cttcggttta ttcgtttact ggttccggta
     2221 cacggtggtc ttgatcctgc gaaccccggc cggtcctgag tacgcaaaac gagtcgctgc
     2281 tgccaaccaa ctgaacttcg tgcaggtccg ccagaagttg cattcctccg tcgatgccgg
     2341 gcaactgggc ttcctctgcg atgctctgca tcgagattat acgtcgctca agtatctgct
     2401 gcgccacgcc gccaccttgc aagccaacgg atacaccgcc gagcagcggc tgttgatgat
     2461 caacttccgc tgtatggccc tctggtgcaa gatgatcctt cggttgcggc cggcggccgc
     2521 gaagctggcg ctgctggaga tgtccggtac actcgagtac ttcgcaaacg caatgggccg
     2581 ccgtttcgcg acgctggccg gcgaagcctc ccaggcctag gctttccgaa gcttgattcg
     2641 cgctgccccc tgctgtatcg taatcctgta acccgttcca gaaatctcgc gtccgctacc
     2701 aacgaggggc tgctatgagc acgtaccatt tcgcacggtt cttagtccgg gcgttcgccg
     2761 gcatttctct aggcctcttc gccctttctg tggggcaggc gcagcaggcg cccgccaagg
     2821 tacaggcctc agcgacggca tctaaccctt ccatcccagt ggatgaaatc attcagaagt
     2881 ttgccgcgaa ggaactggag ttcaagcggg cccgcgacaa ctacacttac cgccagacgg
     2941 tgcgtgtgct cgagctggac gacgacggcg cggtgcgggg gcggtacgag atggtatcag
     3001 atatcatttt cagcccttcg ggcaagcgca tggagcgagt cgtgcgcgcg cccatgtcca
     3061 ctttgaagcg catttccttg gatcccggcg acgaacagga tctgcgcaac gtgcagccgt
     3121 tcgtcctgac gacggaagat atcccaaaat acaacatctc gtatgttggg ccgcagaaag
     3181 tcgatgaact tgacacgtac gtgttcgccg tcaagcccaa aaaattggag cccggccagc
     3241 gctatttcga aggtcagatt tgggtggatc agcaggattt gcagattgtc aaaacctacg
     3301 ggaagggcgt gggtctgcaa aagaaaggct ccgaccacca gtacccgcgc tttgagacct
     3361 accgcgatca aatcgatggc aaatactggt tcccggtcta cacgcgcgcc gacgatgtgc
     3421 tgcatttcca gaccggcgat cagcgggtgc gcatgaccgt gaagtatgaa gattacaagc
     3481 agttcaagag ccaggtcggc attgaatacg agggcgtcgt aaacgacaag aagccggaag
     3541 acgagaagaa gaaataggcg ctggccaaac tcaccgctgc cttccggggc ggtccccttt
     3601 ggattttagg aacgattgcg tttcgaagcg cgtcactttt gcggttggat ccgcttaacg
     3661 ccgcgcggcg gatgaatttc gaggcgggac gcctggaact tcggatcatt gagctttatg
     3721 ttcgtgtagg tggaaagccg gtagtcgccc ggcgtaacgt cgtaaaactt ctgctgcacc
     3781 ggctgccagt ccactgtgga aacccacatc tcgattcgcg ggacgtgctg aagcagttcg
     3841 gcggattttg gaatcagctc cagcttgacg gcaggctgcc ccacgacgct ttccggcttt
     3901 tccattcgca cctgataatt cgcggcgaga tcccggcctg aagtgccgaa agagagcagc
     3961 cagaactggt cgcgaagggt ggcgtatttg gtgacgtcgt attcttcgat ggttgctatc
     4021 ttgggccggt aaatttccgc ttttttttcg gtgaccgaga cgtaatactt atccggactg
     4081 gtgaactcga tgagcatccg ggagactcgc ggcttcaccc gcatgaccca gatggtgcct
     4141 tcatccaccg tctgatcgtt cacgatcgcc gtatatttca cgctgcggat gtgcgccgtg
     4201 aggttgcgga aggcgactga agcggcgtcc attttcgcca ggacggactc gaggctgggc
     4261 gcttcaggag tttgcgccgg caggagcgcg aaaaggaacg ccgcgatcag aaaagtgcgc
     4321 acgcttagaa ttgagcccgg accaagtacc ctttcaagct gccttcactg tctttggcag
     4381 gttcgacttt caatataccg acctggcggc cggtgcgttt ctggagcaag gcatggattt
     4441 tcgcttcgag ctgctccgga ggcgcatccg cgaccagttc cgggtcgatc cagaacacgg
     4501 ggcggccatt ttcgctgggc agcattacaa tctgctgcgg attgggaatc ctcggctggt
     4561 cgcggaacca tgctctcggt gttgcgaaaa tgaaaaccag aatcaggccg acgatcacat
     4621 cgtattgcca gcttccgcgg cgatattgcc aaaaaaccgc cggccggatc agcttccaga
     4681 tcttgcgcat agcatcagtg tagcagaggg gcgaaccggc ccggcaggcc gggaaagagc
     4741 cgttcccgca aacgctggga ttttcccaag gccaaggcaa acgcttcaac ttgaatgaga
     4801 taccggagag aaacaaagcg cgattctgct ataatctgac atatgcgtgg aatggtgcgg
     4861 gaagcgcgcc ggttcatccg gctggcgagt catatccaat taatgcctgt ccttgaaagc
     4921 ggcgaagagc agcttctggt tggaggccag gcggtgatcg agggcgtgat gatgcgcgcc
     4981 ccccacagct attgtgttac ggttcgccgg cccgatgggt ccgtagcagc cgagcaggct
     5041 ccgctccagc gcccatccga gaagcaccgg atttttaagt accccgtttt tcgcggcttg
     5101 ggaacgctgg gccaggccat ggctctggga atcaaggcgc tgcgtttttc ggccgacacc
     5161 gccattgaaa aagatccggc gcagaaagac ggcaaggccg gccagatctc gaacggaatg
     5221 atcacggcta acgtcctgtt ctccgttttg tttttcctgt ttctttacaa gttcgtaccg
     5281 ctgtttctgg ccacgcaact gaaggatcgt tttgcggcgc tggacaacta tttcttgttt
     5341 aatctgatag acggatcggt gcggatctct attttcctgg cttttctcgg ggtcatttcg
     5401 cgaaccaaag acatccgccg ggtgtttgag taccacgggg cagagcacaa agtggttttc
     5461 aactttgagt ccggtcagcc ggtgagcgtt cccaacgccc agtgcttttc cactttgcat
     5521 ccgcgatgcg gcacgagttt tttgttggtt gtgatggtga tcagcatgtt gatttacgcc
     5581 ctcgttccgt tccagggctt cgggttgcgg cttctctcga gggtgctgct cctgccggtg
     5641 atcgtgggat tgagctatga ggtgattcgt ttcgcggcgc gccgccaggg gacgctgtgg
     5701 gcctccatgg tagctcccgg gctctggctt cagaaagtca ccacccggcc cccgtctgac
     5761 gaacaggtgg aaatttccat tctgtcgctg gaaggagcta tggctctcga aaagcagcag
     5821 ggcggagaac tggtgatcgc gtaaactgcc ttcagccatc agcgctcagt aatcgggagg
     5881 accgcaacgg gtgttcggcc cgagccgccg gccgctgaca gcttttatat gcaatacata
     5941 gccaaactcg atcaactgga ggccaggttc gaggaactga cgcagcagct tgcggacccc
     6001 agtgtgatcg ccgacaacgc gcgttatcgc aaatcggcca aagcgcacag cgatctggcc
     6061 gaggtagtgg agaaatatcg cgcgtggaag gcggttcaga gaaacatgga gggaacgcgg
     6121 ccgatgctgg aggaggcgga tccggaaatc cgggaaatgg cccagcagga attggcctcg
     6181 ctcgagcagc gcgcgcatga cctcgagcag gagctcaaga tcctgcttct cccgaaagat
     6241 cccaacgacg aaaaaagcat cgttctcgaa attcgggccg gcacaggggg cgatgaggcc
     6301 acactgttcg cggcggaaat tttccggatg tacacccggt atgcggaaag ccagggctgg
     6361 cgcgtggagg tcacctcgtt gagcgagtcc tcgctcggcg gaatgaaaga agttatcgcg
     6421 ctggtctcgg gggaaaaggt ttacagcaag atgaagtacg aaagcggggt gcaccgcgtg
     6481 cagcgcgtcc ctgcaaccga gcagcagggg cgcgtgcata cgtcggccgt cacggtggcg
     6541 gtgctgccgg aggccgatga agtcgaggtg gagatcgacc cgaaggacat tcggattgac
     6601 acgttctgtt cttccggccc cggcggccaa tcggtgaaca caacgtattc cgcggtgcgc
     6661 atcacgcatc tgcctactgg actgatcgtt tcctgccagg acgagaaatc gcagatcaag
     6721 aaccgggcga aggccctccg ggtgctccgg tcgcggcttt atgaaatgga gcggcagaaa
     6781 cagcatgagg ccatggctga ggagcgccgc agcatggtcg gctcgggcga ccgcagcgaa
     6841 aagatccgca cgtataactt tccgcagaat cgcgtgaccg atcaccgtat cggcctcacg
     6901 ctgtaccagc tcgatcaggt gatggacggc aagctcgacc cgttgatcga cgcgctggta
     6961 acacactacc aggctgagaa actgaagcag gaagcggttc tcatataggg ctccccgcag
     7021 aaccgtggac ggggcgcccg cgggcgtccg gagctgtgat tcgcttgaac gtttttaccg
     7081 ccctccggca gggtacggaa atcctcgaaa aagaaaacat cagcgctccc cggctcacgg
     7141 ccgaggttct gctggcgcat gcgctgcgct gcgagcgggt cgatctgtac gcccatccgg
     7201 agcgcgaact caccctcgcc gagcaggcgc actacgggcg ctgcctgcac gaacggctgg
     7261 cccgaaagcc ggtccagtac atcaccggcc gccaggagtt ttacggccgg atgttttctg
     7321 taagcccggc ggtgctgatc ccgcggccgg aaacagagct ggtggtcgag gcggccctgc
     7381 gctatgcggc ggccgcccgg cgcattctcg acgcagggac cggctcgggc tgcctggcgg
     7441 tcacactgaa gaaggaatgg ccgcacgcgg caaccttcgc cagcgacatt tccgttgccg
     7501 ccctgcgcgt cgcagaagag aatgcccggc ggctcgaagc ggacgtgcac ttcttttgcg
     7561 ggaatttact ggatgcatgc cgggctcagg agatggactt gatcgtttcc aatccgccgt
     7621 atgtg
//
LOCUS       DASREK010000145        11170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1230529, whole genome shotgun sequence.
ACCESSION   DASREK010000145 DASREK010000000
VERSION     DASREK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11170
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230529"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFA54_07240"
     CDS             complement(<1..799)
                     /locus_tag="VFA54_07240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS50636.1"
                     /translation="MPPLITVIIPTLEGGENLEKCLAALASQTFTRFEIIVVDNSGGA
                     AGVPEAPAGGRVRVIRNETNAGFGAAVNQGIQAAGGELICTLNDDAFPEPHWLEELAS
                     ACAREVSAGMFASQIRLASAPERLDSAGMQIYWDGSSKQRARMALAADFSTSEEVLFP
                     SACAALYRAAMLKKIGGFDPDFFLYCEDTDLGLRARRAGFRCLYVPSAVVYHRYSGSA
                     GRASAAKAFYVERNRLYTVIKNFPVWLWPAVPFFSFYRYVIHLYGLVA"
     gene            1087..2235
                     /locus_tag="VFA54_07245"
     CDS             1087..2235
                     /locus_tag="VFA54_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZS50637.1"
                     /translation="MTGLLRIGVNALFLIPEGVGGTEIYIRNLLAALAEIDDHNQYFV
                     FLSAEAAAAAAPVTPSAANFHEVRCAVRATNRPARLLWEQLSLPAKVAALRLNVLFSP
                     GFTSPWFTPGCPKVTCIHDLQHMRQPQNFGVFELAAWRALVWISARFSRQIITVSENS
                     RRDILQIYRLPASRVEVVKHGVEPVFWQLRSHPAADGRPLPAPYLLSVSTIHPHKNWG
                     RWLEAYGKLAAEGWPHHLVIAGLRGKYSEQLARVIESQGLTDRVHLTGWLSRVELLSF
                     FRFADGLVFPSTFEGFGMPVLEAMAAGIPVACSDIPPLRETAGDAALFFDPYSVDSIA
                     EAVRKLVGEPELRRQLVEQGLAAAAAFSWTRAAEQTLAILRRAASRAD"
     gene            2508..2894
                     /locus_tag="VFA54_07250"
     CDS             2508..2894
                     /locus_tag="VFA54_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZS50638.1"
                     /translation="MQKAELAKYKAMLEAKRDELARGQRNRADIAIEKTPDLLEEVQL
                     TADRELAIRNLHRESGLLRSIRAALARIADGTYGICLNCEQEIKPKRLDAVPWAAYCV
                     QCQELADRQELDHKEPSELVTDAEAA"
     gene            complement(3045..3512)
                     /gene="tadA"
                     /locus_tag="VFA54_07255"
     CDS             complement(3045..3512)
                     /gene="tadA"
                     /locus_tag="VFA54_07255"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HZS50639.1"
                     /translation="MSESPVRTHEEFMRAALELARQAGQACEVPVGALVVMGNDVVGR
                     GANSPVSLSDPTAHAEMLALRDAARTLGNYRLAGATLYVTLEPCAMCAGALVAARIDR
                     VVFGARDLRYGAVRSKFRLADSELLNHRVTVMEGVLAAECLSVLQKFFRERRE"
     gene            3651..4874
                     /locus_tag="VFA54_07260"
     CDS             3651..4874
                     /locus_tag="VFA54_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS50640.1"
                     /translation="MDPEPASKVFGELFSINAVEKREDAMWKYLPFTFKNALRNRRRT
                     VLTVLSVSVSLFLLGTLIAVYFAFYHREGPPEAALRAAVHHRVSLAFALPEFYAARIQ
                     QIPGVKEVCRASWYGGVYIDHRPRNNFARFAVDADKIFPIRPELRVDPGQRAAFERER
                     TAAAVGRSIAEPLGLKLGQRITLQGDIYPGNLELVIRAIFEGENDAVMYFHRDYLEES
                     LPPARRGWTGMILVLTDSAEAVPRVSNAVDEMFRNSPQQTKTESERAFQLSFVSLLGN
                     IKLILLGICSAVTFAILLVSANTMAMSVRERISEVGVLRTLGFTSEKVLAMIMAEAVM
                     MALAGGLIGSFLAMGMVQWVSKMPIFFVQGMHMPPAALGISLGVALLIGLASSLIPAL
                     SAARMPVTEALRHTG"
     gene            4894..6060
                     /locus_tag="VFA54_07265"
     CDS             4894..6060
                     /locus_tag="VFA54_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS50641.1"
                     /translation="MAIPFSYNLRNLLERKTTTIMTALGIALTVAVMLGILGILAGLQ
                     KSFQATGDPLHVLVLRKAATAELTSLVTRENFQVLRAKKGIAVENGELMASLELITVI
                     SLPFQGSTETANVNVRGLPPVGIRMRARSVRLSEGRWFTPGQRELAIGRGVAGSYSHV
                     GIGQRLQVGRGYYTIVGIFDAGQTAFNGEIWGDANQLGTDAGRVSTLSSVLLQAAGAE
                     AIPALRESIDNDQQLSLEAIPETDYYLRQTQSGQPLRYLGIFVAIVMAVGSSFAAMNT
                     MYAAVTRRTREIGVLRLLGFSRASILFSFMVESLLLTLLGGALACLAVLPFNGLASRM
                     GNLVTFSQSLFQFRVTPGMIVIGMGFASLMGLAGGLWPARAAARKDVLAALRDL"
     gene            6066..7427
                     /locus_tag="VFA54_07270"
     CDS             6066..7427
                     /locus_tag="VFA54_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS50642.1"
                     /translation="MDTNQYDEALNSLKIDRSRRGRGGPRWAAVWILTGAAGIAALLL
                     WLLFQGRSQAIEVQTIRASASTAPAIADPGVVLNATGYIVAHHRIEVTSKVMGKVAWI
                     GVEKGDKVRQGQVLVRLEDQEFRAQLQQARGNVANLEAKLREMQAGSRPEEIAAARAN
                     VAQAEADLENYRISLERTRGLVAAGIVSRQNLDDAQARYDGQKAKVESLQKNYELVKL
                     GPRIEEIDAVRGQLEQARGQLAYAETMLDATLIRAPVSGTILERAVEVGEFVTTNFVG
                     DRGAKGYVAALADLNDLQVELDINQDDFAKLGPRQRGIVTTDAFPDRKYDGVIDEISP
                     AADRQKATVQVKVKILKPDEYLRPEMNARVAFLADLKTAAGAAQQAPIVIPSSAVRDG
                     KTVFLARDGKAFERPVKVRATSARGSEISEGLADGEEVIINPPPNVKNGDRIKPKAKS
                     AAL"
     gene            7424..8122
                     /locus_tag="VFA54_07275"
     CDS             7424..8122
                     /locus_tag="VFA54_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS50643.1"
                     /translation="MSDHAAVEAAGLTKEFIRDSFRVVALENANLTVERGEFLALMGP
                     SGSGKSTLLHLIAAIDRPSRGRVIVLGQDLQQLSDKQVAQWRNENIGFIFQTFNLIPV
                     LTALENVELPLLLTHLNKKERREHAAVALRLVGLADRMHHYPRQLSGGQEQRVAIARA
                     IVTDPAIILADEPTGDLDAQSAQEILTILSKLNSQHGKTVIMVTHDPNAARYAGRTRH
                     IEKGALLPEDAAPG"
     gene            8135..9613
                     /gene="glpK"
                     /locus_tag="VFA54_07280"
     CDS             8135..9613
                     /gene="glpK"
                     /locus_tag="VFA54_07280"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HZS50644.1"
                     /translation="MYILSLDEGTTSARAIVFDREARALAMESAPIECRYPQPGWVEQ
                     DPEQIWRAQLETARRALARSGVAASRLAAIGITNQRETSVVWDRKTGCPIAPAIVWQC
                     RRTAGYCQSIAARGGGCAARIAARTGLIIDAYFSASKIRWILDNIPGASRKAESGELA
                     FGTVDSWLIYKLTGGRVHVTDRSNASRTMLLNLDGTWDPDMLELFGIPRAMLPRVVRS
                     AEIVGMTDPELFEAEIPIAGIAGDQQSALVGQACFRPGLSKNTYGTGCFFLLHTGRRP
                     VASRNRLLTTVAASAGKQPEYALEGSVFVAGAAVQWLRDSLGLIRKAEDTAALAASVP
                     DTGGVYFVPAFVGLGAPHWNASARGAITGLTRATTSAHLVRATLEAIAYQTRELVEAM
                     EADAGEPLAELRADGGAAGNDFLMQFQADILGRPVVRPANIETTALGAAFLAGLATGV
                     WPGIQAIESFWKVERRFEPNLPVDRREELFDGWKRAVAACCK"
     gene            9631..11055
                     /locus_tag="VFA54_07285"
     CDS             9631..11055
                     /locus_tag="VFA54_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50645.1"
                     /translation="MPERLSPSPIASRIFPYNEPTMLRRMLSASVALCALCSAQSADE
                     WKAQALALKSKGDIAGALAALERAAERAPASAELQDEIGVVFDGLNRPPEAIAHFARA
                     AQLNPNFAPAQYHLGTAYWLKNEREKGLAALQRAAALDPKNPEYRRRLGTAYYELARY
                     AEAARELREAAELQPADGETWNRLGLARERAGDLWNAIEAYRKAVEAEPKNTNARNNL
                     ALLLVQMHSYDKGIEEFRKILELEPQNGVARMNLGFALLRKGDLDQAAAHFQEATRRN
                     PNLAAGYYNLGLALKEKKDLKGAIAALKKAIEIDSRFAEAHYTLGAIYWQQGKLSETA
                     GEMRAAIASRPGYSEAWYLLGAALRQRGDLPDAGKALREAIRLDPHNPDCYTVLAEIL
                     KKQGDDAGSRAMAVHAVQIQREKQAQQAAAQHLSAGAERLAKGDLAGAERQFRLALAA
                     APNWDQAKRYLEETERRKEAARGK"
BASE COUNT         2185 a         3379 c         3587 g         2019 t
ORIGIN      
        1 cggcaaccag gccgtaaagg tgtatgacat agcggtaaaa ggaaaaaaac ggaaccgcag
       61 gccacagcca aaccgggaag ttcttgatca cggtatacaa acggttgcgc tccacataga
      121 atgccttcgc cgccgaagcg cgccccgccg agcctgagta gcgatgataa accaccgccg
      181 agggaacgta aaggcaccgg aagcctgccc ggcgggcgcg caagcccagg tcggtgtcct
      241 cgcagtataa gaagaaatcg ggatcgaaac cgccgatttt cttgagcatc gcggcgcggt
      301 aaagggcggc gcaggcgctc ggaaacaaaa cctcttcact tgtcgaaaaa tccgcggcca
      361 gagccatgcg ggcccgctgc ttgctgctcc cgtcccaata gatctgcatg ccggcggaat
      421 cgagccgttc cggcgcgctg gcaagcctga tttgtgatgc gaacatgccc gcgctcactt
      481 cccgggcgca ggccgacgcc agttcctcca gccaatgcgg ctcggggaag gcgtcatcgt
      541 tgagtgtgca gatgagctcg ccgccggccg cctggattcc ctgattgacc gcagcgccaa
      601 agccggcatt ggtttcattt cgaatcacgc gcacgcgtcc gccagcgggc gcttccggaa
      661 ctcccgctgc gcctccgctg ttgtcaacca caatgatctc gaagcgggtg aaggtttggg
      721 acgcgagcgc cgccaagcac ttttcgagat tctcgccgcc ttcgagggtg ggaataatca
      781 ctgtgatcag aggcggcaaa ctatagttat atcccggatt cggcaacgaa gtagaaccgc
      841 ttgctcgcgc gcggctgccg gatactcgaa atcgggccgg caagcggtaa tgcacggtct
      901 ggcggcgcag ccgcaacgct ggcgggcgca ggcgatcggg ccggctagcc gcctatcgaa
      961 atttcacgag ttgattccag gcacgccgcc cggataatcg ccccccaagg acggatcttc
     1021 cagtcggccc tcgggcaacg acgagcccag tggcgcgata gggtaggata tcatcgctaa
     1081 tcagccatga cgggtctctt acgcatcggg gtgaatgccc tatttttgat tcccgagggc
     1141 gtgggcggga ccgagattta tatccggaat cttctggccg ccttggccga gatcgatgat
     1201 cacaaccagt atttcgtgtt cctaagcgct gaagcggcgg cggccgcggc cccggtcact
     1261 ccctcggccg ccaattttca tgaggttcgg tgcgcagtcc gggccacgaa ccggccggcc
     1321 cgcctgcttt gggagcagtt aagcttgccg gccaaggtcg cggcgcttcg gctaaacgtg
     1381 ctgttctccc caggcttcac ttcaccgtgg ttcacacctg gatgcccgaa ggtgacctgc
     1441 attcacgact tgcaacacat gcggcagccg cagaatttcg gagttttcga gctggcggcc
     1501 tggagggcgc tggtgtggat ctccgcccgc ttctcccggc agattatcac cgtctccgag
     1561 aattcgcgcc gcgacatcct ccagatctac cggttgccag cctcgcgcgt ggaggtagtg
     1621 aagcacgggg tggaaccggt attttggcag ctcagatccc atcccgccgc tgacgggcgg
     1681 cctctccccg ctccatatct cttgtcggtt tcgacgattc accctcacaa gaactggggc
     1741 cgctggcttg aagcctacgg caagctggct gcggaaggct ggccccatca cctggtaatc
     1801 gccggactgc gagggaagta ctcggagcaa ttggcgcggg tgatcgagag ccaaggtctg
     1861 acggaccggg tacacctgac aggctggctc tcccgggtgg agctgttgtc ctttttccgg
     1921 tttgccgacg gcctggtctt cccttcgaca ttcgaggggt tcggcatgcc ggtgctggag
     1981 gcgatggcgg cgggaatacc agtggcctgt tcggacatcc cgccccttcg ggaaaccgcc
     2041 ggggacgcgg ccctgttctt cgatccctac tcggtcgatt cgatcgccga ggcggttcga
     2101 aaactggtcg gtgagccgga gctgcgccgg caactggtcg agcagggttt ggcggccgcc
     2161 gccgccttta gctggactcg cgccgcggag caaacgctgg cgatccttcg gcgcgcggcc
     2221 agccgagccg attgagacac gtgaatcccg ggctgcgctc cctggcggcc gtttcgttca
     2281 catttttcta aaggaatttt tgaaaaaggg aagcctcggg aactgctaca atctctcaag
     2341 ccgaatcagg gaagctgact cggggacagc gcaagcgtgt atttttacca accgtgatcc
     2401 gccttagcca tccggtgcgg gacggaaggt ttcgattttc aaccaccaac ggcccgcaac
     2461 caaaccggaa gaacgtgcat caaaaggtcg ttggaaggag aacggacatg caaaaggccg
     2521 agttggccaa atacaaggca atgctcgaag ccaagcgcga tgagctggcg cggggccagc
     2581 gcaaccgcgc cgacattgcc atcgaaaaga ccccggattt gctcgaagag gtgcaactga
     2641 ctgcggatcg cgagctggcc atccgcaacc tgcaccggga gtccggcttg ctccggagca
     2701 tcagggcggc tttggcgaga attgccgatg gcacctacgg catctgtttg aactgcgaac
     2761 aggagatcaa accgaagcgt ctggatgcgg ttccgtgggc tgcttattgc gtgcagtgcc
     2821 aggagctggc agaccgccag gagctggacc acaaagaacc ctccgaattg gtgacagacg
     2881 ccgaggcggc atagagcgcc gccgcccttt ccccggatct tagtttcccc agaaacaatt
     2941 caagagcaag ccgggccttt aggcagtgca aaatgcttcc gggcgggcag ccgcaccttt
     3001 ttgaggccgg aaacgagggc ggcgaagttt ggtgtgctcg tgccctattc gcgccgctca
     3061 cgaaagaatt tttgcagcac cgagaggcac tcggcggcca agacgccttc catcaccgtg
     3121 acgcggtgat tcagcaattc agaatccgcc aggcggaact tgctgcggac tgcgccgtac
     3181 cggaggtctc tggccccgaa gacgacccga tcgatgcggg ccgccaccag cgctccggcg
     3241 cacatcgcgc aaggctcgag ggtcacgtat aaggttgcgc cggcaaggcg ataatttccg
     3301 agagtccggg ccgcgtcccg caaggcgagc atctcggcgt gagccgttgg gtctgacaag
     3361 ctcaccggag aattggcgcc gcggcccacc acatcgtttc ccattactac gagagcgccg
     3421 accggcactt cacaggcttg cccggcttgg cgggccagtt cgagcgccgc tcgcataaat
     3481 tcttcatggg tccgaaccgg actttcactc acggccgcat ccgccggcaa gccctacggc
     3541 ccctgccggc gtccagcaca atggtacact gaatcgaagc agccgagcgg cgggggaaat
     3601 gtgtccggtt tgggcggaga aggggcgctc tccggggcgg accaacgcga atggacccgg
     3661 aaccggcatc caaagttttt ggggaacttt tctcgatcaa cgccgtagaa aaaagagaag
     3721 acgcgatgtg gaaatacctg ccattcactt tcaagaacgc tcttcgaaac cggcgccgca
     3781 ccgtactcac ggttctaagc gtcagcgtct ctctgtttct gctcggcacc ctgatagccg
     3841 tctatttcgc tttctaccac cgggaggggc cgccggaggc ggccctgcgc gcggccgttc
     3901 accatcgcgt ctcgctggca ttcgctctgc cggaattcta tgcggcgcgg attcagcaga
     3961 ttcccggcgt caaggaggtt tgtcgcgcct cctggtatgg cggcgtttat atcgaccaca
     4021 ggccccgaaa caactttgcc cgctttgcgg tggacgccga caagattttt ccaatccggc
     4081 ccgaactgcg cgtggatccc ggccaacggg cggcgtttga acgcgaacgg accgccgcag
     4141 ccgtcggacg ctcgatagcc gagccgctag gcctcaagct cggccagcgg atcactttac
     4201 agggggacat ttatccgggc aacctggagt tggtcatacg cgccatcttc gagggcgaga
     4261 acgacgccgt catgtacttc catcgcgact acctggagga gagcctgcct ccggcgcgcc
     4321 ggggatggac gggcatgatc ctggtcctca ccgactcggc agaggcggtg ccgcgtgttt
     4381 cgaacgcagt ggacgagatg tttcgcaact cgccgcagca gaccaagacg gagtcggagc
     4441 gggcgttcca gttgagcttc gtcagcctgc tgggaaatat caagctgatc ctgttaggca
     4501 tttgctcggc ggtgacgttt gccattttgc tggtttcggc gaacacgatg gcgatgtcgg
     4561 tcagagagcg catttccgag gtaggggttc tgagaacgct cggattcacc tcggagaaag
     4621 ttctggcgat gattatggcc gaggcggtga tgatggcgct ggccggcggc ttgatcggca
     4681 gttttctggc tatgggcatg gtccaatggg tcagcaaaat gccgatcttt ttcgtacaag
     4741 ggatgcacat gccgccggcg gcgctgggca tcagcttagg cgtggcgctt ctgatcggtt
     4801 tggcgagctc gctgattccc gccctgagcg cggcccggat gccggtcaca gaagctctcc
     4861 ggcacaccgg ctgacggggc gcggcaacga aagatggcta tcccgttttc ctacaatttg
     4921 cgaaacctgc tcgaacgcaa gacaaccaca atcatgaccg ccctgggcat cgctcttacg
     4981 gtggccgtga tgctgggcat tctcggcata ttggcgggcc tgcaaaagtc atttcaagcc
     5041 acgggggatc ctctgcatgt gcttgtgctg cggaaggcgg cgacggcgga gctcacgagc
     5101 ctggtgacgc gcgagaactt tcaggtgctg cgggccaaaa aaggaatcgc cgtagaaaac
     5161 ggcgagttga tggcgtcact cgagctgatc acggtcatca gcctgccctt ccagggttcg
     5221 accgagacgg ctaacgtgaa cgtacgcggg ctcccgccag tgggcattcg gatgcgcgcc
     5281 cggagtgtaa ggctgagcga gggccggtgg ttcacccccg gccagcgcga attggccatc
     5341 ggccggggcg tagccgggag ctacagccat gtcggcatcg gccagcggct gcaagtcggg
     5401 cgcggctatt acaccatcgt gggcatcttc gacgccggcc aaaccgcctt caacggggaa
     5461 atctggggcg atgccaacca actgggaacc gatgccggcc gggtctccac cctcagctcg
     5521 gtgctgctac aggcggccgg cgcagaggcc atcccggcgc tccgagaaag tattgataat
     5581 gaccagcagc tcagcctgga ggcgattccc gagaccgact attatttgcg ccagacccaa
     5641 tccggccagc ccctgcgcta tctcggcatt tttgtggcca tcgtgatggc cgtgggcagt
     5701 tccttcgccg caatgaacac catgtacgcg gcggtgaccc ggcggacgcg ggaaatcggc
     5761 gtgctgcgct tgctcgggtt ttcgcgcgcg agcatcctgt tttcgttcat ggtcgaatcg
     5821 cttctgctca ccttgctggg aggcgcgctg gcgtgtctgg cagtgctgcc atttaacggc
     5881 ctggcaagcc ggatgggcaa tctggtgact ttcagccaaa gtcttttcca gttccgtgtt
     5941 acgccgggta tgattgtaat aggtatgggt tttgcatcac tgatgggtct ggcgggcggg
     6001 ttgtggccgg cccgagccgc ggctcgcaag gacgtgctgg ccgcattacg ggatctgtaa
     6061 tggtcgtgga caccaatcaa tacgacgaag cgttgaacag cctcaaaatc gaccgaagcc
     6121 gccgcggccg aggcggcccg cgatgggcgg cagtctggat tttgacgggc gccgcgggga
     6181 tcgcggccct tctgctctgg cttcttttcc agggccggag tcaggcgatc gaagtccaaa
     6241 ccattcgggc cagcgcctcg acggcgccgg ccatcgccga tcccggcgtc gttctaaacg
     6301 ccaccggtta catcgtcgcg caccacagga tcgaggtcac cagcaaggta atgggcaagg
     6361 tggcgtggat cggcgtcgaa aagggggaca aggttcgcca gggccaggtg ctggtacggc
     6421 tggaggacca ggagttccgt gcccagctcc agcaagccag gggcaatgtg gcgaacctcg
     6481 aagcgaagct gcgcgaaatg caagccggat cgcgtcccga ggaaatcgcc gctgcgcggg
     6541 ccaatgtggc gcaggcagag gccgatcttg aaaactaccg gattagtctc gagcggaccc
     6601 gcgggctggt agcggcgggc attgtttccc ggcaaaatct cgacgatgcc caggcccgat
     6661 atgacggaca gaaggcaaag gtggaatccc ttcaaaaaaa ttatgagctg gtgaaactcg
     6721 ggccccgcat cgaggaaatc gacgccgtac gcggacagct cgagcaggcc agaggccaac
     6781 tggcctacgc cgagacgatg ctggacgcga cgctgatccg ggcgccggtc tcgggaacca
     6841 ttctggagcg ggccgtcgaa gtcggcgaat tcgtaaccac aaatttcgtc ggcgatcgtg
     6901 gggcgaaagg ctacgtagcc gcgctggccg acttgaacga tttacaggtc gagctggaca
     6961 tcaatcaaga tgatttcgcc aagctgggtc cgcggcagcg cggcatcgtt acaaccgacg
     7021 ccttccccga tcgcaaatac gatggtgtga tcgacgaaat atcgccggca gcggaccggc
     7081 aaaaggccac cgtgcaggtc aaagtcaaga tactaaaacc ggatgaatat ctgcggccgg
     7141 agatgaatgc gcgcgtggcg ttccttgccg atttgaaaac cgcggccggc gccgcgcagc
     7201 aggccccgat cgtgatccct tcctccgcgg tccgggacgg caaaacggtc ttcctggcgc
     7261 gggacggcaa ggctttcgag aggcctgtca aggtccgggc cacgtcggct cggggctccg
     7321 aaattagcga agggctggcg gatggagaag aggtgatcat aaacccgccg ccgaatgtga
     7381 aaaacggcga ccggattaag cccaaggcga aatccgcagc cttatgagcg atcacgccgc
     7441 cgtcgaagcc gccggcctta ccaaggaatt tattcgcgac tccttccgcg tggtggcgct
     7501 cgaaaacgcc aatcttactg tggagcgcgg cgagtttctg gccctgatgg ggccttccgg
     7561 ctcgggcaag tccacgctgc tgcacctgat tgccgcgatc gaccgcccgt ccagaggccg
     7621 ggtgattgtg ctgggacagg atctccaaca actgagcgac aagcaggtcg cgcaatggcg
     7681 taacgaaaac attggcttca tctttcagac cttcaacctg atacccgtgc tgacggcgct
     7741 cgaaaacgtt gagctgcccc tgctgctgac gcatctgaac aagaaggagc ggcgcgagca
     7801 cgccgccgtg gcgctacgcc tggtgggctt ggccgaccgc atgcatcatt acccgcggca
     7861 gctttccggc ggccaggagc agcgcgtggc gatcgcgcgc gcgatcgtca ccgatcctgc
     7921 catcatcctc gccgacgagc ctaccgggga cctcgatgcg cagtcggcgc aggagatcct
     7981 taccattctt tccaagttga acagccagca tggaaagacc gtcatcatgg tcacgcatga
     8041 tccgaacgcg gcccgctatg ccggcaggac gcgccatatt gagaagggcg cgctgctccc
     8101 ggaagatgcg gcgccgggct aaacggcgac taccatgtac attctgtcgc tcgacgaagg
     8161 caccacgagc gcccgcgcca ttgtcttcga ccgtgaagcg cgcgctctcg ccatggaatc
     8221 agcgcccatc gaatgccggt atccgcagcc gggctgggtc gagcaggacc cggagcagat
     8281 ctggcgggcg caactcgaga cggcgcgacg cgccctcgcg cgctccggcg tcgcggcgtc
     8341 ccggctcgcg gcgatcggta ttaccaatca gcgcgaaacg tccgtcgtat gggatcgcaa
     8401 gactggatgt ccaatcgccc ccgccattgt gtggcaatgc cgccgcacag ccggctattg
     8461 ccagtccatc gccgccaggg gcggcggctg cgcggccaga atcgccgccc gcaccggact
     8521 gatcatcgac gcctattttt ccgccagcaa gatccgctgg attctcgata acatccctgg
     8581 cgcttccaga aaagccgaaa gcggcgaact ggccttcggc actgtagatt cctggttgat
     8641 ctacaagctg accggcgggc gcgtgcacgt caccgaccgc tcgaacgcgt cgcgtaccat
     8701 gcttctgaac ctggacggga cgtgggatcc cgatatgctc gaattgttcg gcattccccg
     8761 ggcgatgctg ccccgggtgg tgaggtcagc ggagattgtg ggaatgacgg atccggaact
     8821 gttcgaggcc gaaattccca ttgccggcat cgccggcgat cagcagtcgg cgctggtggg
     8881 ccaggcctgt ttccggcccg gcctgtcgaa aaacacgtac gggacaggct gttttttcct
     8941 gttgcacacc ggccggcggc cggtcgcatc ccgcaaccgc ctgctgacga cggtcgcggc
     9001 gtcggccgga aagcagccgg agtacgcgct cgagggaagc gtgttcgtgg ccggagcggc
     9061 ggtgcaatgg ctgcgcgatt cgctgggcct gattcgaaag gccgaggaca ctgcggcctt
     9121 ggctgcctct gttcccgata ccggcggagt ctatttcgtt ccggccttcg tcggcctggg
     9181 cgcgccgcac tggaacgcgt cggcgcgagg cgcgattacg ggcttgactc gcgccaccac
     9241 atcagcgcac ctcgtgcggg cgaccctcga agccatcgcc taccagacac gcgagctggt
     9301 ggaggcgatg gaggccgacg ccggggagcc gctggcggag ctgcgagccg atgggggcgc
     9361 tgcgggcaac gacttcctga tgcaattcca ggcagacatt cttgggcgac ctgttgttcg
     9421 tccagcgaat atagagacta cggcgctcgg agccgcgttt ctcgcggggc tggcgacagg
     9481 cgtctggccg ggcattcagg cgatcgaaag cttctggaag gttgaaaggc gcttcgagcc
     9541 aaacctgcca gtggaccggc gcgaagaact cttcgacggc tggaagcgag cggtggctgc
     9601 ctgctgcaag tgaccgaacg gttccgccga atgccggagc gtctctctcc ctcgcccatc
     9661 gcatcgcgca tttttcccta taatgaaccc acgatgctcc gccggatgct cagcgcgtcg
     9721 gttgcccttt gcgccttgtg cagcgcgcaa tcggcggacg aatggaaagc ccaggcgctg
     9781 gccctgaaat cgaagggcga tatcgcgggc gcgctggctg cgttggagcg ggccgccgag
     9841 cgcgcaccgg cctccgccga actccaggat gaaatcggcg tcgtatttga cgggctgaac
     9901 cgcccgccgg aagcgattgc ccacttcgcc agggccgcac agctgaatcc gaattttgcc
     9961 cctgcgcaat atcatctggg cacagcctat tggttgaaga atgagcgcga gaaagggctg
    10021 gctgcgctgc aacgggccgc tgcgctcgat ccgaagaatc ctgagtacag acgccggttg
    10081 ggaaccgcct attacgaact ggctcgatat gccgaggcgg cgcgcgaact gcgggaggcg
    10141 gcggagctcc agccagcgga cggcgaaaca tggaaccgcc tagggctggc tcgagagcgg
    10201 gcgggcgacc tgtggaacgc gatcgaggcg tatcgcaagg cggtggaggc cgagcccaag
    10261 aatacaaacg cccggaataa tctcgcgctg ttgctggtac agatgcactc gtacgacaag
    10321 ggcatcgagg agtttcgcaa gatcctcgaa ctggagccgc agaacggcgt ggcccgcatg
    10381 aatctcggct ttgccttgct gcgaaaggga gatctggatc aggcggcggc ccactttcag
    10441 gaagcaaccc gccggaatcc gaatctggcc gctgggtatt acaacctggg gctggctctc
    10501 aaggaaaaaa aggatctgaa gggagcaatc gcggctttga aaaaagcgat cgagattgat
    10561 tcccgcttcg ccgaagctca ctatacgttg ggcgccattt attggcagca gggaaagctt
    10621 tcagagaccg cgggcgaaat gcgggccgcg atcgcttcgc gtccgggcta ttccgaggcg
    10681 tggtacctgc taggagccgc actgaggcaa aggggagact taccggacgc ggggaaggct
    10741 ctgcgggaag cgatccggct ggaccctcac aatccggatt gttataccgt tcttgccgag
    10801 attctgaaaa agcagggaga tgacgctggc agcagggcta tggcggtcca cgccgtccag
    10861 atccagaggg aaaagcaggc gcagcaagcc gcggcccagc acttgagcgc cggtgccgag
    10921 aggctcgcaa agggcgatct tgccggcgcc gagcggcaat tccggctggc gctcgccgcc
    10981 gcccccaact gggaccaggc gaaacggtac ctggaggaga cagagcggcg aaaggaagca
    11041 gcgcggggaa aatgatgttg gcccgccggc gccgcagcgg atcagcggat ctgaaggtta
    11101 cgatttcgtt tccagttctc cccggttttc cacttttccg ttctttgcgg aggcagagcc
    11161 ctctgatgaa
//
LOCUS       DASREK010000146         7254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2381063, whole genome shotgun sequence.
ACCESSION   DASREK010000146 DASREK010000000
VERSION     DASREK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381063"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFA54_07290"
     CDS             <1..274
                     /locus_tag="VFA54_07290"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZS50646.1"
                     /translation="TIGIGAGPDCDGQVLVWHDLLGLHFGHTPKFVRRYADLAGEIER
                     ALDRYVRDVRAGSFPSDSESYHLPEAEQQKLALQQEKAAPATTGTR"
     gene            361..1194
                     /gene="panC"
                     /locus_tag="VFA54_07295"
     CDS             361..1194
                     /gene="panC"
                     /locus_tag="VFA54_07295"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZS50647.1"
                     /translation="MESTAEMQARALQARAAGQTVGLTPTMGALHEGHLSLVRAARRE
                     SEFVVVSLFVNPTQFGPTEDFDRYPRDFEGDRRKLEAEGVDVLYAPPAAEIYGPGFQT
                     YVEVEELGKRLDGASRPGHFRGVATVVAKLFHITQPHRAYFGQKDAAQLAVIRRLVRD
                     LDFRVEIVACPIVREPDGLAMSSRNAYLAAAERKAAAVLYRSLTRARICYESGERSGP
                     VLRRILLDTLASEPLARVDYAEVVDPDSLLPLARVDRRALAAVAAWFGETRLIDNVLL
                     G"
     gene            1277..1531
                     /locus_tag="VFA54_07300"
     CDS             1277..1531
                     /locus_tag="VFA54_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HZS50648.1"
                     /translation="MKPMFLPDVENNARGNGPYAELIRQMKLGNVPIPQIFHLFAFKP
                     EATEHLSRFTQAVMRGPSPLSPGLRELIAAFTSQQNRCPF"
     gene            1583..1894
                     /locus_tag="VFA54_07305"
     CDS             1583..1894
                     /locus_tag="VFA54_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50649.1"
                     /translation="MDAVLADYRTAPLSAAEQALFAFLEMVNRNSPDITEADVDKVRR
                     AGWTDEALYDAISVCALFNFYNRWIDATGVHGMTPEAYAASGKRLAERGYVLSASEDE
                     K"
     gene            complement(2069..2236)
                     /locus_tag="VFA54_07310"
     CDS             complement(2069..2236)
                     /locus_tag="VFA54_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HZS50650.1"
                     /translation="MLYWSLLFLVIALVAALLGFGGIAAAAAGIARLLFILFLILFLV
                     SLIAHLARRAR"
     gene            2525..5812
                     /locus_tag="VFA54_07315"
     CDS             2525..5812
                     /locus_tag="VFA54_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50651.1"
                     /translation="MRKSHLSFTILFVVLASLSSVVAWGQAAGTISGKVISPSGAAVP
                     EAAVSITNTGTGASQRVLTGPDGGFLVSGLAPGTYKVAVENPGFTTAVRENIQLAADS
                     PVTLELVLEAGAPGQTKVEAQSSTSAVQDSSGQKSQLFGERQITDTPIIDRNRQQLQE
                     LVTGVTPPEAMLAPALDPQRNRQYSVNGQQALGVLQSRKLDGSQNIETYLGISDHIPT
                     NEAIQTFNVVTSNPRAMYGITAGSSEPVFNRTGTNEIHGDVFEFNSFSWLRARPVFDN
                     SPNPAPTYAFNQFGGSVGGPIVKNRIFFFGAYEGDIQRGQNTLITTVPTAAFRSGDFS
                     GVPGLTLFNPATGTSSGAGRFPFVGNLIPASAISPMASALLPFIPLPNRPGTEDNFMR
                     NVPFHNDGNRADGRVDFRASDHTSFFARYAFSRFFALDESPLGSLIGNTQEGNMHAHN
                     GALNLTHVFSPGVTTEGRFSMYRYDDKLYGPSLNSPLAATIGVSNLPSSALPSILISG
                     MAPIGTKLGYPTFISSMNGLPVIGPTAGFPGTSVNNDFEWTSNWNITRGRHNVNWGVD
                     VWHIRSDGFRNPLFSPAGEFDFGAGATGVPGVALAASGLFPSAFAAFLAGAPTQVGVQ
                     SFAVTPSYRTTYYSGFVNDVVRVGGRLTLDLGLRYDIFSPLSTRRAGDAAIYNPADNT
                     LRLVGVDGVSDRGNVKYDFDNVAPRFGFALKLTDKTVVRGGYGITYFPAATMFAGGYL
                     NPIAFTAQAGAPGSFAVAGGVISLPAPAAPSSATAGLIAAPPNQTFVFGPPHPETPYV
                     QSYSVSVQQDFGANTLLDIAYIGSLGRELPFSREINFVGPGATDFPLAAFGRTASTIE
                     RGYGLNNNYNSLQVNLRKQFQHYISFIGSYTYSKALDYSSNPFVPIIGATARNYGPAD
                     YDRTHIFSLSHVIQIPIGAGSGRLNSGRIGEALGGWQLNGIFRWATGTPFSVLADPAA
                     CGCPGLPYAYANAVQPISSNAEAGFNPAAFAAPSGSIGNTGRNILRGPGFKTYDLSLF
                     RSFSLRERAKFELRGEAYNLFNTPLFANPVNVVGSANLGLVTHTLSPNMINSNNREFH
                     LAAKIIF"
     gene            complement(5809..6435)
                     /locus_tag="VFA54_07320"
     CDS             complement(5809..6435)
                     /locus_tag="VFA54_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50652.1"
                     /translation="MSRVLPAGVLLAALSASIAGAQTHVAVFMAFDEKPSPVSVAEMK
                     AETARILRSAGLLLDWRPLSQRLAAEAFGGLAVMRFKGACRADKFQAPFGPLERESET
                     EPVTLGWTAVRDGRVLPFSEVECDQIRNTLGPMRLAQKDLMLGRALGRVVAHELYHIV
                     AETARHGRAGVAKPFHTPDDLRRDGASFEEEDRAAIFRQRGPVPPLLR"
     gene            complement(6484..>7254)
                     /locus_tag="VFA54_07325"
     CDS             complement(6484..>7254)
                     /locus_tag="VFA54_07325"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS50653.1"
                     /translation="WERTGATPNDMRGENIEKLYRLRTSRRWRERLYRLLRPPDPFVS
                     NPREHAIDAPLGKWNLYVGGAGSRVPGYVNVDLFPLPGVTVAASLEQLPFPGNLFQRV
                     ECDAVLEHVGNPDAAMREIERVLAPGGYAHLVVPFCHPYHQYPSDFRRFTPDGLKQMA
                     GSLEIAGAGWRTGPTATLLVFLLEYIKIWLPWRPARMLAHGVAGWLLFPLRYLDVLLF
                     HFSRSGQIGNHHYLWLRKPFPGGDPAAQHRTENHAPAP"
BASE COUNT         1417 a         2199 c         2321 g         1317 t
ORIGIN      
        1 gactatcggc attggcgcag ggccggattg cgacgggcag gtactcgtgt ggcacgatct
       61 attagggctg catttcggcc atacccccaa attcgtgcgg cgctacgccg atttggcggg
      121 ggagatcgag cgcgcgctcg accgctacgt ccgcgacgtc cgggcaggat cgttcccgtc
      181 ggattcggag agttaccacc tgccggaagc cgagcagcag aaactggcgc tgcaacaaga
      241 gaaggctgcg ccggcgacaa cgggaacgcg gtaacaacgg gcgcgccggc ccggcggatc
      301 catcgaaaat atccggggca aagaccactt acctgcctgc ttcgtttgcg catgacgatt
      361 gtggaatcaa cagcggaaat gcaggcgcgc gccttgcaag cgcgcgcggc gggccaaacc
      421 gtggggctga cgccgactat gggcgctttg catgaaggcc atctgagcct ggtgcgagcc
      481 gcccgccgcg agagcgagtt cgtggtggtt tcgctgttcg tgaacccgac gcaattcggc
      541 ccgacggaag atttcgaccg ttatccgcgg gatttcgaag gcgaccggcg caagctggaa
      601 gccgagggcg tggatgtact ctacgcgccg ccagcagcgg agatttatgg accgggcttt
      661 cagacgtatg tggaggtcga ggaactgggg aaaaggctgg atggggcgag ccggccgggt
      721 cactttcgcg gcgtggcgac cgtggtcgcg aaactcttcc acatcaccca gccgcaccgc
      781 gcatacttcg gccagaagga cgccgcgcaa ctggcggtca tccggcggct ggtcagagac
      841 ctggactttc gggtagaaat cgtagcctgc ccgatcgtcc gcgaaccgga tggtctggcg
      901 atgagctcgc gaaacgcgta tttggcggcc gccgagcgga aagcggcggc ggttctttac
      961 cggagcctca cccgggcgcg catatgttac gagtcgggtg agcggagcgg ccccgttcta
     1021 cggcgtattt tgctcgacac gctcgcctca gagccgctgg cgcgagtcga ttacgcggag
     1081 gtagtggatc ccgattccct gctgccgctt gctagagtag accggcgcgc gctggcggcg
     1141 gtggcggcct ggtttggaga gacacgtcta atcgacaacg ttctgctcgg ttagctgata
     1201 gttggatccg ggcggcggcc cttgcgggtt cgcccggccc gtagtctctc ttgccgccct
     1261 gctatactgc acccttatga agccgatgtt cctgccggat gttgaaaaca atgcgagggg
     1321 taacggtccg tacgccgaat tgatccgcca gatgaaactg gggaacgtcc cgattcccca
     1381 gatttttcac cttttcgcct tcaaaccgga ggctaccgag cacctgtcgc gcttcaccca
     1441 ggccgtgatg cgcgggccgt cgccgctgag cccgggtctg cgggagttga tcgccgcttt
     1501 cacctcgcag cagaaccgct gcccgttttg aatcggcagc cacgccgcag ttgcggcgga
     1561 actgctcggg gactcgcgcc tggtggacgc cgtgctggcc gactaccgga cagcgccgct
     1621 ttccgccgcg gaacaggcgc tattcgcatt cctcgaaatg gtgaacagaa attcgccgga
     1681 catcaccgaa gccgacgtgg acaaggtgcg ccgcgcggga tggacagacg aggcactcta
     1741 tgacgcgatc agcgtttgcg cactcttcaa cttttataac cgctggatcg acgcgacagg
     1801 cgtgcacggc atgactccgg aggcgtatgc ggcttcgggc aagcgcctgg ccgagcgcgg
     1861 ctacgtttta agcgcttccg aggacgaaaa atgaccggtc aagcgacgct gccgcgcctg
     1921 gtccttttac acaggcgaat aggcctgacc tcctgccgta agcaatatct ttcctgaaac
     1981 agctccgatt ttccattcga aacgagtcgc caccgaacgg gctacggccg cggcagcgca
     2041 tgcccccggc gcgcaaaccg gcgcccatct agcgagcccg gcgcgccaga tgcgcaatca
     2101 ggctcaccag gaacagaatc aggaagagaa tgaacagcag ccgcgcgatt ccggccgccg
     2161 cggcggcaat tccgccgaac ccgagcagcg ccgccaccag cgcgatcacc agaaacagca
     2221 aagaccaata aagcatagac gggttctcct tgcagcgccc gagggggaag cgtagcgacg
     2281 ccggcgcagc ctgcctatgc aaagaaaatg ccagtgtgag attcctcaat tcaaaaatgt
     2341 tcaattctga ccttcgggcg gcggatttgt aaacagaaaa acggggagcg cggcgccggc
     2401 gagatttttt gtcacccaaa atgcagcgat acgtctgtaa gaacgaaaag gggtcttcag
     2461 gaatgcgtgc tctttctggg gacgcgaaac atgatggtaa cggtttggct ggaggtttag
     2521 agaagtgagg aaatcacatt tgtcgtttac tattctgttc gttgtcttgg cttccctatc
     2581 ttctgttgtc gcatggggac aagcggcggg aacgatttcg gggaaagtga tatctccgtc
     2641 gggcgccgct gtcccggagg cggcagtcag cattactaat actggaaccg gcgcttcgca
     2701 acgcgtgcta acgggtcccg acggcggctt cctggtatcc ggactcgctc cggggaccta
     2761 caaagtcgcc gtggaaaatc ccggattcac cacggcggtt cgcgaaaaca tacagttggc
     2821 ggcggactcg ccggtaaccc tggagctggt gctggaggcg ggcgctcccg gacaaaccaa
     2881 agtcgaggcg cagtcctcca cctcggccgt gcaggattcc tcgggccaga agtcgcagct
     2941 tttcggcgaa cggcaaatta cggacacgcc aatcatcgac cgcaaccggc agcagttgca
     3001 ggaactggta accggcgtta caccgccgga agcgatgctg gcgccggctc tggacccgca
     3061 gcggaaccgg caatactcgg tgaacgggca gcaggctctc ggcgtgttgc aatctagaaa
     3121 gctggacggc tcgcaaaaca tcgagaccta tctcggaatc tcggatcaca tcccgaccaa
     3181 cgaagcgatc cagaccttca atgtggtcac gagcaatcct cgcgccatgt acgggatcac
     3241 ggcgggatcg tcggaacctg tcttcaaccg gacggggacc aatgaaattc atggagacgt
     3301 tttcgaattc aacagcttta gctggctgcg ggctcggccg gtattcgaca actcgcccaa
     3361 tccggccccc acttacgctt tcaaccagtt tggcggcagc gtgggcgggc cgatcgtcaa
     3421 aaaccggata ttcttcttcg gcgcgtatga aggcgacatt cagcgcgggc agaacacgct
     3481 gatcaccacg gttccgaccg ctgcgttccg ctcgggggac ttcagcggcg tgccggggct
     3541 gacgctgttc aatccggcca caggaacttc cagcggcgcg gggagatttc cgttcgtggg
     3601 caatctgatt cccgccagcg cgatcagccc gatggcatcg gcgctgctgc ccttcattcc
     3661 gcttcccaac cggcccggca cggaggacaa cttcatgcgg aacgttcctt ttcacaatga
     3721 cgggaacaga gcggacggcc gggtggattt ccgggcgagc gatcacacgt cttttttcgc
     3781 ccggtacgcg ttctcgcggt tcttcgcgct ggatgagtct ccgttgggat cgctgatcgg
     3841 gaacacgcaa gaaggcaaca tgcatgcgca caacggggct ctgaatctca cgcacgtgtt
     3901 cagcccgggc gtcactaccg aaggcagatt cagcatgtac cgctatgacg acaagctcta
     3961 cggcccgagt ctgaacagcc ctctggcggc gacgatcggg gtcagcaacc tgcccagctc
     4021 ggcgctgccg agcatcctga tttccggaat ggcgccgatc gggacgaagc tgggttatcc
     4081 gaccttcatc agctcgatga acggtctgcc agtgatcggg ccgacggcgg gtttcccggg
     4141 gacgagcgtc aacaacgatt ttgagtggac gagcaactgg aacatcacgc gcggccgcca
     4201 caacgtcaac tggggcgtgg acgtgtggca catccggtcc gacgggttcc gcaatccgct
     4261 gttctcgccg gcgggcgagt tcgatttcgg cgcgggagcg acgggggttc ccggagtggc
     4321 gctcgcggct tcgggcctgt ttccgagcgc gtttgccgcg tttctggccg gagctcccac
     4381 gcaggtgggc gtgcagagct tcgcggtcac tcccagctat cgaaccacct attacagcgg
     4441 attcgtgaac gacgtcgtgc gggtgggcgg gcgtctgacc ctcgatttgg gtttgcggta
     4501 tgatatcttc tcgcctcttt ccacgcgccg ggcgggggac gcggccatat ataacccggc
     4561 ggataacacg ctgcggctgg tgggcgtaga cggcgtcagc gaccgcggca acgtgaaata
     4621 cgatttcgac aacgtggcgc cgcgcttcgg cttcgccttg aagctgacgg acaagaccgt
     4681 ggtgcgaggc ggctacggca tcacttactt cccggcggcg acgatgttcg ccggcggcta
     4741 tctgaacccg atcgccttca ccgctcaagc gggagcgccc ggctctttcg cggttgccgg
     4801 cggggtaatt tccttgcccg cgccggcagc gccgtcttca gcgacagcgg ggctgattgc
     4861 ggcgccgccg aaccagacct tcgtgtttgg gccgccgcac ccggaaacgc cttatgtgca
     4921 gagctacagc gtttccgtac agcaggattt cggcgccaac acgctgctgg acatcgctta
     4981 catcggaagc ctgggccgcg agctgccgtt cagccgcgaa atcaacttcg taggcccggg
     5041 ggcgaccgac tttccgctcg ccgccttcgg gcgcacggcc agcacgatcg agcgcgggta
     5101 tggtcttaac aataactaca actcgctgca agtgaacctg aggaagcagt tccagcacta
     5161 tatcagcttt atcggctcgt acacctacag caaggcgctc gattacagca gcaatccgtt
     5221 cgtgccgatc attggcgcaa cggcgcggaa ttacggcccc gccgattacg accgcacgca
     5281 catcttctcg ctgagccacg tgattcagat tcccatcggc gcgggctcgg gccggctgaa
     5341 ctcggggcgg attggagagg ccctcggggg atggcaactg aatggcattt tccgctgggc
     5401 gacgggaacg ccgttctccg tgctggccga tccggcggcc tgcgggtgcc cggggctgcc
     5461 gtacgcgtac gccaacgcag tgcagccgat atcctcgaac gccgaagcgg gcttcaaccc
     5521 ggcggcattt gcggcaccgt cagggagcat agggaacacg gggcggaaca tccttcgcgg
     5581 accgggcttt aaaacctacg atctctcgct gttccgctca ttctctctgc gggagcgcgc
     5641 gaagttcgag ctgcggggag aggcctacaa cctgttcaac acgccgctgt tcgccaaccc
     5701 ggtgaacgtg gtgggatccg cgaatctagg cctggtcacg cacacgctga gcccgaacat
     5761 gatcaactcc aacaaccggg aattccacct ggcggcgaag atcatcttct aacgcagcaa
     5821 cggcggaacc ggaccgcgct gtctgaaaat ggcagcgcgg tcttcttcct caaaggacgc
     5881 gccgtcccgg cggagatcat ccggcgtgtg gaacggcttg gcgactcccg cacgtccgtg
     5941 cctggcggtc tcagccacaa tgtgataaag ctcgtgagcg acgactcttc cgagggcccg
     6001 gccgagcata agatctttct gggcgagccg catgggtcct aaggtattgc gaatctgatc
     6061 gcattccacc tcgctgaagg ggagcacgcg gccatcgcgc acggcggtcc aaccgagcgt
     6121 cacgggctct gtttcactct cgcgttccag aggtccgaac ggggcttgga atttatcagc
     6181 gcggcaagcg cccttgaatc gcatcacggc gagaccgccg aaggcctcgg cggcgagtct
     6241 ctggctgaga ggccgccaat cgaggagcag gccggcggat cgcaatatcc tcgctgtttc
     6301 cgccttcatt tcggcgacgg aaaccgggga aggtttttcg tcgaacgcca taaatacggc
     6361 gacatgagtt tgggcgcccg cgatggaggc gctcagagca gccagcaaga cgccggccgg
     6421 cagcactcgc gacatgcggt caatctagca cgtttcccaa aaaacgtaaa agaaaattcc
     6481 gatttacgga gccggagcgt gattttccgt acgatgctgt gcggccgggt cgcccccggg
     6541 gaagggtttc cgcagccaaa gataatggtg gttgccgatt tggccggagc gggagaaatg
     6601 gaacagcagc acatcgaggt accgtagcgg gaagagaagc catccggcaa cgccgtgggc
     6661 cagcatgcgc gccgggcgcc aggggagcca gatcttgatg tactcgagga gaaagaccag
     6721 aagcgtggcg gtggggcccg tgcgccagcc ggcgccggct atttccaggc ttccggccat
     6781 ctgcttgagc ccatcgggcg taaagcggcg gaaatcggat ggatattgat gatagggatg
     6841 gcagaaaggc acgaccagat gggcgtagcc gccgggcgcg agcacgcgct cgatctcgcg
     6901 catagcggca tctgggttcc cgacgtgctc cagcacggca tcgcattcga cgcgctgaaa
     6961 cagattgccg gggaacggca attgttcgag cgaggcagcg acggtcactc cgggcagcgg
     7021 gaaaagatcc acgttcacgt agccggggac gcgggatccc gctccaccca cgtagagatt
     7081 ccacttcccg agcggggcgt cgatcgcgtg ttcgcgggga ttggagacga acggatcggg
     7141 cggccgcaag agccggtaga ggcgctcccg ccagcgccgg gaggtgcgca ggcggtaaag
     7201 cttttcgata ttctcgccgc gcatgtcatt tggcgtcgcg ccagttctct ccca
//
LOCUS       DASREK010000147        17818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1891512, whole genome shotgun sequence.
ACCESSION   DASREK010000147 DASREK010000000
VERSION     DASREK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17818
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891512"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1334
                     /locus_tag="VFA54_07330"
     CDS             312..1334
                     /locus_tag="VFA54_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZS50654.1"
                     /translation="MRSAFFAIPAFLALPLLCLAQTPPSISDGDPIATIGGQPVYERD
                     LAPLIAPQLRQLRNQEYQIKSRALDNLIGQRVLETEAKKRGLTADKLLEQEVDSKIGE
                     PTEGEIEGYYLAQRERIGRPLAEVHSQVEQAVKEAKLQKARQDYMNALREKAGVEILL
                     RPLKTEVAYDPARVKGDPSAPITIVEFSDFQCPFCRRVEPTLTELLAKYKGRVKLAYR
                     DFPLREAHPQAEIAAEGARCAGDQGKFWEFHDVLFSNPSKLDENSLVEHARALGLDEK
                     QFRTCLSSGKFKNQVEADLQDGARAGVAGTPGFFINGASLEGAQPLAAFEKIIESELA
                     AGGRRP"
     gene            1483..2310
                     /locus_tag="VFA54_07335"
     CDS             1483..2310
                     /locus_tag="VFA54_07335"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZS50655.1"
                     /translation="MLRIGIHTSIASSLASAAEKAAALGCNTFQIFSASPRMWRGSKL
                     DPAEVRLFREAREKHDLFPLVIHDNYLINLPSADPVVRERSIEAFRAEMQRAMALGAE
                     YLVAHPGSYRGQTPEQAIQVFAGSLAAAARGLRCDGLSVLIENTAGGGCALGSEPAEL
                     LELRRLASERVDFPIGFCIDTAHCFAAGLDFFAVLQAVGLEAVQVIHCNDSRSGFGSR
                     LDRHEHIGKGGIGLEGFRRILSDRRLRRKAFILETPVEKPGDDRRNVRVLKALAKTR"
     gene            2390..4837
                     /gene="leuS"
                     /locus_tag="VFA54_07340"
     CDS             2390..4837
                     /gene="leuS"
                     /locus_tag="VFA54_07340"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZS50656.1"
                     /translation="MEYNHKTIEEKWQARWEQADLFRAETDPSREKYYVLEMLPYPSG
                     TLHMGHIRNYSIGDALARYKWMRGYSVLHPMGWDAFGLPAENAAIKNNRPPREYTLSN
                     IAHMKRQHRRFGFSYDWSREVSTCEPEYYRWNQWFFLKMLERGIAYRKHARVNWCPKC
                     NTVLANEQVVNGCCWRHEDTPVEQRALEQWFFKITDYAEQLLADMAELERGWPERVLT
                     MQRNWIGRSEGARIDFRLDASGEEGAGETITVFTTRIDTIYGATCVILAPEHPLMAKL
                     TAGRPDLQAAAARLAESRQGRDPADLVKEGFFLERFALNPFNGERLPVWSANFVLMEY
                     GTGAIMAVPAHDERDFEFCTKYGIPVRTVIVPAEGQEAPAAGAFTEDGVLVNSGPFSG
                     LSSAEARRKMTAYGREHCFAEPAVTYRLRDWGVSRQRYWGTPIPVIYCRQCGVVPVPE
                     EQLPVLLPLNVQITGMGGSPLRSVPEFLNVKCPRCGGPAERETDTMDTFVDSSWYFYR
                     YCDPHNSRRPFDSEKIRYWFPIDQYIGGVEHAILHLIYSRFFTKVMRDLGLVQHSEPV
                     KRLFTQGMVIREGAKMSKNKGNVVDPETIVNQYGADTARLFSLFAAPPEKDLDWNDAG
                     VEGMYRFLGRVFRFVTREDRPQPRAGEGPADREVLRKLHQTIRKVTEDFETRWHFNTS
                     IAAIMELVNELYQHEANLSAAALNQALPALVLLLAPFAPYAAEELWFRLGRRGPVLRA
                     PWPEFDPALAAEEEAEIVLQVNGKVRSRLVAPRQTPKEQLEKLALADPKLQAHLAGKR
                     IVKIVSVPDKLVNVVVH"
     gene            4969..5811
                     /locus_tag="VFA54_07345"
     CDS             4969..5811
                     /locus_tag="VFA54_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZS50657.1"
                     /translation="MKFRIALGWLLLASLAGAEDARLRGGNLLGCETYSFRSLIRAGK
                     LDPLTVPELYRKLGIRGISYNDIFFKSFDDDYVDRVKAAAKKANRTIFCFVMEGNLVS
                     AGEEQRRRQIEIDKQKLRVARRLGAPLVRVNIGNPGRQENASDAMVVERAVAAFKELL
                     PLAQQLGIRMSIENHGGLSGKAENIVQIIQQTDAKWVGALLDFGNTPPASRYQDAAQM
                     APYALTTHVKNGEGDIVDVDIPRLLEILKKNRYKGPISIEYELNGDPVQGVQELKAVI
                     LKNW"
     gene            complement(5892..7529)
                     /locus_tag="VFA54_07350"
     CDS             complement(5892..7529)
                     /locus_tag="VFA54_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZS50658.1"
                     /translation="MRLAFVAAFLGLLSGCRHAPAPAPEPEISAARIAAHTQFLSDDL
                     LEGRGTASRGGMLASAYIATQFRLLGLEPAGDDGAYFQRVPLVGVQTLPSSTLSWNHH
                     ALRLLVDYVGVNQRQQKDLALDAPAIFVGHGISAPEFQWDDYKNTDVKGKVVVLFTNE
                     PPSTDPKFFGGPALTYYGRWTYKYEEAARRGALACLIVHTDKTAGYGWQVVRNSWSGK
                     EPYVKLGPNQQALAFAGWVTEEIGNQIFRSIGKTATEMLRLAETRSFQPVALNIRIRG
                     RIVSDVSPMDTRNVVAKLPGSDPKLKHEAVLYTAHWDHLGIGPAVNGDNIYNGAVDNA
                     TGCGILLETARAFAHRNPKPARSILFASVGAEEGGLLGSQYLGEHPPIPAGKIAAGLN
                     YDGIFPFGRTMDISLGGYERTTLKGLVEQTAKAFDLKIEPDPHPEQGHYYRSDHFSLA
                     RVGIPAFSLDVGSDFIGKPPGWGQKAFDEYNDKHYHQPSDEFDPNWDFSGLAELARFG
                     FRLGAAIANLPQLPSWQPGDEFLPARERSWGEASVRR"
     gene            7613..9466
                     /gene="asnB"
                     /locus_tag="VFA54_07355"
     CDS             7613..9466
                     /gene="asnB"
                     /locus_tag="VFA54_07355"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZS50659.1"
                     /translation="MCGIAGYCGPARRFNQEKLELMLRAIEHRGPDDAGIFRADLPEQ
                     DLSVWLGSRRLAIQDLSPAGRQPMTDAATGSAIAFNGELYNFRDLRSELEQAGCRFCS
                     RCDTEVALRSWCAGGIGSVAQWRGMFGAAVWDAPSQALWLLRDPWGIKPLYYRRDGES
                     LAFCSEIRGLLAADLAPRELSRRGVEDFLQFGAPQDPVTILRGVYALLPGHALCWSRG
                     RTEIRRYAARPSPIQGGPEAIEPMLRKIVRQQLISDVPAVVFLSGGADSSVIALLARE
                     EAGADMHTMTVIFDENSYSERDFARRVAAHAGTRHHEVPLSAGDACCQALEAISRMDQ
                     PSVDGVNTYVISAAAKRSGFTVALSGVGGDEFFGGYSTFRRTALLEKLGRIAGRMPAL
                     AGFASHVLTRHGTAGAQKLGVYLTQPSQHPYFLQRTLFFPAQIGEICACGARETEADC
                     LAGERLQNILDEAGALDRLNQVSLLEARTYMANTLLRDSDQMSMAHSLELRVPFADHL
                     LAAFLLGIPGEKKGFHRAGKLWLRQAFGNRLPPEVFEREKMGFTLPFAVWLKGPLAKE
                     VSQTLDRNPHRIWRRDAALRVWNDFLRGRAGWSRPWALYVLSRWAEKTLPV"
     gene            complement(9519..9824)
                     /locus_tag="VFA54_07360"
     CDS             complement(9519..9824)
                     /locus_tag="VFA54_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50660.1"
                     /translation="MRMLMNVKFPHEPFNTAIRDGSAGSKIRRVLDEVKPEVVYFTEQ
                     DGHRGATLIVNVNDPSQIPALAEPWFLLFDADIELRVAMTADDLGRAGLEALGKKWA"
     gene            10001..11866
                     /gene="iolD"
                     /locus_tag="VFA54_07365"
     CDS             10001..11866
                     /gene="iolD"
                     /locus_tag="VFA54_07365"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HZS50661.1"
                     /translation="MTRRLTTAQAIIEFLKHQYVERDGTERQFFGGCFGIFGHGNVAG
                     IGQALQQNPDFRFYLCRNEQAMVHTAAAFAKMNNRLRTLVCTTSIGPGATNMITGAAA
                     ATVNRIPVLLLPGDIFARRNVAPVLQQLECSASQDISVNDCFKPVSRYWDRIQRPEQI
                     ITALPEVMRVLTSPADAGACTLALPQDVQAEAFDFPARLFEKRVWRIQRPRGDAETLR
                     RAAQWIRSSKQPLIIAGGGVTYSEATDALSRFAAQTGIPVAETQAGKGSLCHDHGQAL
                     GAMGVTGTPGANIVAREADLVIGIGTRYSDFTSASKTAFQNPDVRFININVCEFDAYK
                     HCALPVIADARAALAELGQALEGYRVSADYRARIDRLRAEWEKEVDRIYSERHGPPVS
                     QGAVIGVVNRISGPRDVVVSAAGSLPGDLHKLWRTRDPKGYHLEYGYSTMGYEIAGGL
                     GVKMADPSREVYVLVGDGSYLMMAQEIVTSIQEGYKLNIVVLDNHGFSSIGGLSQACG
                     SGGFGTEYRYRVDGSSGASLSGEPIPVDFAANAASLGAHAVRAQTTDQLAEELETARR
                     RDRTTVVVIETDINARVPGYESWWDVPIAEVSEMESVKAARAAYEPARRKERYYL"
     gene            11955..12911
                     /gene="iolC"
                     /locus_tag="VFA54_07370"
     CDS             11955..12911
                     /gene="iolC"
                     /locus_tag="VFA54_07370"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HZS50662.1"
                     /translation="MEVTVLGRVGYDLYSEEPHTPLPQARRFSRYLGGSSANMAVGLS
                     RLGAEVGIVAALGDDALSDFLRDFLRAERVDVSRVKTVPGYLPSLCLTEVSPPDRFPQ
                     VFYRRDPADTQLDIDAGDLAYIASGRMFITNGTSLCASPSRESTYRAMEHARAAGCRV
                     VLDVDYRAMSWTRPEHAGLAIRLALPFVDVLIGNDLELKLVTGASELDDAVAALRKSR
                     LPMLVSKLGSAGTRVYCGDSSVFLAPYSVEVVSTIGAGDGFASGFLYALLRGMPVEEC
                     LHYGNAAAAIVVSRLSCSEAMPTLAEVEQLIEEQKARLTLQA"
     regulatory      13161..13356
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            13412..14440
                     /locus_tag="VFA54_07375"
     CDS             13412..14440
                     /locus_tag="VFA54_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS50663.1"
                     /translation="MHLNELTRTLIDIESITENEKAVGDFLYEYLRPLAARFGGELER
                     MEVEPGRFNVFACWGKPEIVLSTHMDTVPPFIRSREDDEFIWGRGACDAKGIAASMLH
                     AAEALLAEGRRGLGVLFLVGEERNSAGARAANRQPRGARCLINGEPTENKLALGSKGA
                     LRLEVEAHGKMAHSAYAELGDSAILKLLDALDALRHIEWPSDPVLGESTCNIGTISGG
                     RAPNVVPDFARAEIMIRLVNNSGELCRKVIAALGHRVETRVVLEVPPVRLGSVPGIET
                     TVVKFTTDIPELTRWGQPFLLGPGTIHVAHTAEERVPKRQLVEAVGLYQKIVKELQDK
                     PPNATRGA"
     gene            14446..15501
                     /gene="asd"
                     /locus_tag="VFA54_07380"
     CDS             14446..15501
                     /gene="asd"
                     /locus_tag="VFA54_07380"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZS50664.1"
                     /translation="METKIEVGVLGATGMVGQHFLRFLDGHPWFRLTWLGASERSAGK
                     RYSDAAPWHLNGGCPESIAALSVEECKPGRAPRLVFSAMDASVAGEIEQEFARAGHVV
                     VSNSRNHRMLPDVPLLVPEINADHLQLIPLQQRARGWKGMIVTNPNCSTVALTMALAP
                     LKQFGITKVIATTLQAISGAGYPGVPSMDIMGNVIPHIGGEEEKMETETQKILGDYVA
                     GAGQIRPLAAAVSAHCNRVPVVDGHTVTVSAALEQKPALEDLLAAFESFRGVPQERKL
                     PSAPARPVVLMREVNRPQPRKDAERERGMACFVGRVRKCPVLDYKLVALGHNTIRGAA
                     GAAVLNAELMYSEGLLD"
     gene            15582..16955
                     /gene="lysC"
                     /locus_tag="VFA54_07385"
     CDS             15582..16955
                     /gene="lysC"
                     /locus_tag="VFA54_07385"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HZS50665.1"
                     /translation="MIVMKFGGTSVESASAIERAACIVRSRLERKPVVVVSAMGKTTN
                     KLLAIARTAVDGNKREAIQLIHELRDFHSHEARQVVSLAERAELDLILDEHFQELTEL
                     IKGLSVLGEATPRSIDAISSYGERLSSHIVARALRHFGLDAVHLDSRSYVITDNRFNQ
                     AAPLYGATYPRLAAALPPLLEQGKVVVMGGFIGATEQGVTTTLGRGGSDFSASIIGAG
                     IGADEIQIWTDVDGMLTADPTILPGGRRVRVISFNEAAELAYFGAKVLHPSTLVPAMN
                     MNIPVFILNSRRPECEGTKIIPQAPPTSNLAKSIACKKNLTVVDVSSTRMLMAHGFLH
                     KIFEIFDRYETPVDMVTTSEVSVSLTIDKTDRLEEIARELSRFAEVNHENHQAIICVV
                     GDNIRYTPGVAGRIFKAVEHVNVRMISQGASLRNVSFVVQESDLKPAVEALHKAFFSE
                     LDPEVFD"
     gene            16952..17665
                     /gene="dapB"
                     /locus_tag="VFA54_07390"
     CDS             16952..17665
                     /gene="dapB"
                     /locus_tag="VFA54_07390"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZS50666.1"
                     /translation="MSAGTSPQPKLAIVGYGRMGKLIEQLSREFGFQVALKLDIDNNV
                     NGSGITRENFREIQVAVEFSTPLVVVENIERISALGVNLVVGTTGWFDQIERVRTAVA
                     RAGTGLVYSPNFSVGMQIFSRLVEAAARCFQAQPDYDAWGYEIHHRMKKDAPSGTLLK
                     LKEVMEKAGYSRPIDLASNRAGAIPGTHVIGFDSEADTITLAHTARSRLGFARGAMKA
                     AQWVMGKKGVYEFSEVLFG"
BASE COUNT         3374 a         5541 c         5532 g         3371 t
ORIGIN      
        1 ggccggcccc tagcaaagac ctgagcaaag aaggaaaaaa cgacgacgaa atgataagga
       61 agcgctgcat ctggcgatcg ccggggcaaa caactaaagt cacgtttggc tagaccgcag
      121 aggtattccc gccaggctcc agcgggcggc gaaaagggcc gcagacgcca gcgcgcggag
      181 gtgaagactg ccatcgctgc gttgcagaaa aatcctgaaa aagcggacca ctaccgccgc
      241 acaacccgac acccccagtt acacccccgg cgttcccgcg ctcacagcgc tgcgatatga
      301 tgattgatac tatgcgatct gcttttttcg ctatcccggc attcttggcg ctgccgctgc
      361 tctgcctcgc tcagacgcct ccttcgatct cggatggcga cccgatcgcc actatcggcg
      421 gccagcctgt ttacgagcgg gacctggcgc ccttgatcgc cccacagctc cgtcagttgc
      481 gtaatcagga ataccaaatc aagagccgcg cccttgataa tctcatcgga cagcgcgtgc
      541 tggaaaccga ggccaagaag cgaggcctga cggccgacaa gctcctcgag caggaagtgg
      601 attctaaaat cggcgagccc acggaaggcg aaatcgaggg atattatctg gcgcaaaggg
      661 agcgcatcgg ccgcccgttg gccgaggtcc attcgcaagt ggagcaggcg gtgaaggaag
      721 cgaaactgca aaaagcccgc caggattaca tgaacgcctt gcgggagaaa gccggagtag
      781 agatcctgtt gcgtccgctg aagaccgaag tggcttacga tccggcgcgg gtgaaaggcg
      841 accctagcgc tccgatcacc attgtcgaat tttccgattt ccaatgcccg ttctgccgcc
      901 gcgtcgagcc caccttgacc gaactgctgg ccaagtacaa gggccgggtg aagctggcgt
      961 accgcgattt cccgctgcgc gaggcgcacc ctcaagcgga gattgccgcc gaaggggccc
     1021 gctgcgccgg cgaccagggc aaattctggg aatttcacga cgttcttttt tccaatccgt
     1081 caaagctgga tgagaatagt cttgtcgagc acgcacgcgc cctcggcctc gacgagaagc
     1141 agttccggac ctgtttgagc agcggaaaat tcaagaatca agtcgaagcc gacctccagg
     1201 acggggccag ggctggcgtc gccggcaccc ctggcttctt catcaacggc gccagcctcg
     1261 aaggcgcgca gccgctggct gccttcgaga agatcatcga aagcgaactg gccgccggcg
     1321 gccggcgccc ctgatcccgc gcgccgcaat gttctgttgt cttcgcaatt atcagtgctg
     1381 tgtttgtctt gtgccgcaat gattcgggcg cgctccctcg ggcgggaagg gagaaaacgt
     1441 cggacctccg agaagggcgg attcgaacgc cgcccggctc ccatgctacg catcggcatt
     1501 cacacttcga tcgcttcttc tctggccagc gcagccgaga aggcggcggc gctcggctgc
     1561 aacacattcc agattttttc cgccagcccg cgcatgtggc gcggctcgaa gctggatccg
     1621 gctgaagtcc ggctcttccg ggaagcccgt gaaaaacacg atctatttcc cttggtcatt
     1681 cacgacaatt acctgatcaa cctgccttcc gccgacccgg tagtgcggga gcgttcgatt
     1741 gaggcctttc gcgcggagat gcagcgggcg atggccctcg gcgcggaata tctcgtcgcc
     1801 catccgggga gctatcgagg gcaaacgccg gagcaggcca tccaggtgtt cgccgggtct
     1861 ttggccgccg ctgcccgcgg gctgcgctgc gacgggcttt ccgtactgat cgagaatacg
     1921 gccgggggcg gctgcgccct gggcagcgag ccggccgaat tactcgaact gcggcggctc
     1981 gcctccgagc gcgtggattt tcctattggt ttttgcatcg atacggcgca ttgcttcgcg
     2041 gccggactcg attttttcgc ggtcctccaa gctgttggcc ttgaggccgt gcaggtgatc
     2101 cactgcaacg attcgcgctc cggtttcggt tcccgcctcg accggcacga acacattggc
     2161 aaaggcggca tcggcctgga gggctttcgg cgcatcctta gcgaccgccg gctgcgcaga
     2221 aaagccttca ttctcgaaac tccggttgaa aagcccggcg acgaccggcg gaatgtccgc
     2281 gtgttgaaag ctctggcaaa aacccgataa gctaggttgc gagccgcggg atgcggatcg
     2341 agggagagct tcgcggcggc gatcggcttc tcgcaaaaac ctttttgaaa tggaatacaa
     2401 ccacaagaca atcgaagaaa agtggcaggc ccgctgggaa caggcggatc ttttccgggc
     2461 ggagaccgat ccttcgcgcg agaaatatta cgttctcgaa atgctcccgt atccgagcgg
     2521 caccctgcac atgggccaca tccgcaacta ctcgatcggc gacgctctgg cccgctacaa
     2581 gtggatgcgc ggctactccg tactccatcc gatggggtgg gacgcattcg gcctgcccgc
     2641 tgagaacgcc gccatcaaga acaaccgccc gcctcgggaa tatacgctct ccaacatcgc
     2701 ccatatgaag cgccagcacc ggcgcttcgg cttcagttac gactggtcgc gcgaggtttc
     2761 gacttgcgag ccggaatatt accgctggaa ccagtggttt tttctgaaga tgctcgagcg
     2821 cggcatcgct taccgcaagc acgcccgggt gaattggtgt ccgaagtgca acacggtgct
     2881 ggcgaacgag caggtcgtga atggctgttg ctggcgccac gaagatacgc ccgtcgagca
     2941 gcgggcgctc gaacagtggt tctttaagat caccgactac gccgagcaac tccttgcgga
     3001 tatggccgag ctcgagcgag gctggccgga gcgcgtgttg accatgcagc gcaactggat
     3061 cggccgctcg gagggcgcgc ggatcgactt ccgcctcgat gcatccgggg aagaaggggc
     3121 cggtgaaacg attacggtct ttaccactcg catcgacacg atctacggcg cgacctgcgt
     3181 gattctcgct cccgagcacc cgctgatggc gaagctgaca gccggaaggc ccgatcttca
     3241 ggccgccgca gcgcgtctcg cggagtcacg gcaaggccgc gatcccgcgg atctggtcaa
     3301 agaaggtttc tttctcgagc gcttcgctct gaatccgttc aacggggagc gcctgccggt
     3361 ctggagcgcc aatttcgtcc tgatggaata cggaactggc gccatcatgg ccgtacccgc
     3421 tcatgacgag cgcgatttcg agttttgcac gaagtacggc attccggttc gcaccgtcat
     3481 cgttcccgcc gaagggcaag aggccccggc cgccggggca tttaccgagg acggcgttct
     3541 cgtcaattcc gggccgttca gcggcctaag cagcgccgaa gcgcgccgga agatgacggc
     3601 ttacggccgg gagcattgct tcgccgaacc tgccgttaca tatcgccttc gcgattgggg
     3661 cgtttcgcgc cagcgctact ggggcacccc gattcctgtc atctattgcc ggcagtgtgg
     3721 tgtggtcccc gttccggaag agcaactccc ggtgctgctg cctctgaatg tgcagatcac
     3781 gggcatggga ggctcgccac tgcgttccgt gcccgagttt ctgaacgtga agtgcccccg
     3841 ctgtggaggc cccgcggaac gcgaaacgga taccatggat acattcgtcg attcctcctg
     3901 gtatttctac cgctattgcg atccacacaa cagccggcgg cccttcgatt ccgagaagat
     3961 ccgttactgg tttccaatcg accagtacat cggcggcgtc gagcacgcca ttctgcattt
     4021 gatctactcc cgctttttca ccaaggtgat gcgggatctg gggctggtcc agcacagcga
     4081 accggtgaag cgcctgttca cgcagggcat ggtcatccgc gaaggcgcca aaatgtccaa
     4141 gaacaagggg aacgtggtcg atccggagac catcgtcaat caatacggcg cggacacggc
     4201 ccgcctgttt tccctcttcg ccgcgccgcc ggagaaagat ctcgattgga acgacgccgg
     4261 ggtcgagggc atgtaccgtt tcctgggccg cgtgttccgg ttcgtaacgc gcgaagatcg
     4321 cccgcagccg cgcgccggcg agggacccgc cgaccgcgag gtgctgcgca agctgcatca
     4381 gactatccgc aaagtcacgg aagatttcga gactcgctgg cattttaata cctcaatcgc
     4441 cgccataatg gaactggtga atgagctata ccagcacgag gcgaacctct cggcggccgc
     4501 cctcaatcaa gctcttccag ctctggtgct tctgcttgcg ccattcgctc cttacgcggc
     4561 tgaagagctt tggtttcgcc tcgggcggcg gggcccggtg ctgcgcgcgc cgtggcctga
     4621 gtttgacccg gccttggccg ccgaagagga agcagagatc gtgctgcaag taaacgggaa
     4681 ggtccggagc cgcctcgtgg cgccgcggca gacccccaaa gagcagctcg aaaaactcgc
     4741 tttggctgac ccgaaacttc aggcccatct ggccggcaag cgcatcgtca aaatcgtttc
     4801 ggtccccgac aagctggtga atgtcgtggt gcattgaagg caggctttgc ttgccgcttc
     4861 aggattgtag cccgattgcg gctttcgaag gcttcgcggt ggaatctcac ggcgcgctgt
     4921 ctgcgtaatg ggcccccggc gtgttgcctc tgtgctacaa agagggctat gaaattccgg
     4981 attgcgctag ggtggctcct gctcgcaagc ctggcgggcg cagaggacgc ccggctccgc
     5041 ggcgggaatt tgctgggatg cgagacgtac agcttccgat cgctgattcg cgccggcaag
     5101 ctcgacccac tcaccgtacc ggagctttat aggaagttgg gcatccgggg catttcttac
     5161 aacgacatct ttttcaagag cttcgatgac gactacgtgg atcgcgtcaa agccgcggca
     5221 aaaaaggcca accgaaccat cttctgcttc gtcatggaag gcaacctcgt cagcgcgggc
     5281 gaggagcagc gccgaaggca gattgaaatc gacaaacaaa agctgcgtgt ggctcgccgg
     5341 ctgggtgcgc cgctggtgcg ggtgaatatt ggaaatccag gccggcagga aaacgccagt
     5401 gacgccatgg tcgtggagcg ggctgttgcg gcattcaaag aactgctgcc gctggcgcag
     5461 cagctcggga ttcgaatgtc catcgaaaat cacggcggcc tttccggcaa ggccgagaac
     5521 attgtccaga tcatccaaca aacggacgcg aagtgggtag gagctctgct cgacttcggc
     5581 aacaccccgc ccgcgtcgcg ctaccaagat gcggcccaaa tggcgccgta tgccttgaca
     5641 acgcacgtca agaacggaga gggcgacatc gtagacgtgg acattccccg cctgctcgaa
     5701 atcctcaaga agaaccgcta caaggggccg atctctatag aatacgagct gaacggagac
     5761 ccggttcagg gcgtgcaaga gctgaaggca gtgatcctga aaaactggta agggcggggt
     5821 tgctggctaa accgccccga gattttccct tgacgcgtcc acatcctcgg cctgggacaa
     5881 gccgccgcgt gtcaccgccg gacagacgct tctccccagg accgttcgcg ggcgggcagg
     5941 aactcgtctc ccggctgcca gctcgggagc tgcggcagat tcgcgattgc ggcgcccagg
     6001 cggaagccga atcgcgccag ttcagccagc ccagagaaat cccaattcgg gtcgaattcg
     6061 tcggacggct ggtgatagtg cttgtcgttg tattcgtcaa acgctttttg gccccatccc
     6121 ggcggcttcc cgataaaatc gctgccgaca tcaagcgaaa aggccgggat gcccacccgg
     6181 gcaaggctga agtgatcgga ccggtagtaa tgcccctgct caggatgcgg gtccggctcg
     6241 atcttcaagt caaaagcttt cgctgtctgc tcgaccagcc ctttcaacgt cgtgcgttcg
     6301 tagccgccaa ggctgatgtc catcgtcctc ccgaaaggaa agatgccatc gtagttcaaa
     6361 ccggccgcaa tcttgccggc gggaatcggc ggatgctcac ccagatattg ggatccaagc
     6421 aagccgcctt cttctgcgcc gaccgaggcg aacaggatcg agcgggcggg ctttggattc
     6481 cgatgcgcaa aggcgcgggc ggtttcgagc aaaatgccgc agccagtcgc attatcgacc
     6541 gctccgttgt aaatgttgtc gccgttgacg gcgggtccga tgccgaggtg gtcccaatgc
     6601 gcagtgtaga gaaccgcctc atgcttcaac ttcggatcgc tgccgggcag ctttgctacc
     6661 acgttgcggg tgtccatcgg cgaaacatca ctgacaatcc ggccgcggat gcggatgttc
     6721 agggcaacgg gctgaaagct ccgcgtctcc gctagccgga gcatttcggt tgcggtcttg
     6781 ccgatcgatc ggaaaatctg gttcccaatc tcttcggtca cccaaccggc gaaggccaag
     6841 gcctgctggt tcggtccgag cttcacgtaa ggctccttgc cgctccagga gttccgcacc
     6901 acctgccatc catatcccgc tgtcttatcc gtatgcacga tcaggcaagc cagggcgccg
     6961 cggcgcgcgg cttcctcgta cttgtaagtc cagcgcccgt aataagtgag cgccggcccg
     7021 ccgaaaaatt ttgggtccgt tgaaggcggc tcattggtga aaagcacgac aaccttgccc
     7081 ttcacgtcgg tgttcttgta gtcgtcccat tgaaattccg gagcgctgat cccgtggccc
     7141 acgaagatcg ccggcgcgtc aagagccagg tccttctgct ggcgctggtt gacgcccaca
     7201 taatccacca gcagccgcag ggcgtgatga ttccagctca aggttgacga aggcagcgtt
     7261 tgcactccca ccagaggaac gcgctgaaag taggcgccgt cgtcgccggc cggctcgagc
     7321 ccgagcagcc ggaactgtgt cgcaatataa gcggaggcga gcatgccgcc ccggctcgcg
     7381 gttccgcggc cttccagcag atcgtcggag agaaactgcg tgtgcgctgc gatgcgggcg
     7441 gcggagatct ccggttccgg cgcgggcgcg ggagcatgac ggcagcccga taacagaccc
     7501 agaaacgcag caacgaacgc caggcgcata ttgaaaagag tagcacggct ataatggctg
     7561 tcagccgcgg gccgcgacaa aatgggtccg cggattttcg tattcacttc tcatgtgtgg
     7621 aatcgccggc tactgcgggc ccgcccgccg cttcaatcag gaaaagctgg agctgatgct
     7681 gcgggccatc gagcatcgcg gcccggatga cgccggcata ttccgcgccg atttgccaga
     7741 gcaagacctc agtgtttggc tgggaagccg ccggctggca atccaggacc tttcgcccgc
     7801 cggccgccaa cccatgaccg acgcagcgac cggcagcgcc atcgccttca acggtgaact
     7861 ttacaacttt cgagatctgc gctcggagct ggagcaagcg ggatgccgct tctgttcacg
     7921 gtgcgacacg gaggtggccc tacgctcctg gtgcgctggc ggaatcggtt ccgtcgcgca
     7981 gtggagagga atgttcgggg ccgcggtctg ggacgcgccg tcacaggctc tctggctgct
     8041 ccgcgatccc tggggcatca agccgctgta ctaccggcgg gatggcgaat ccctggcctt
     8101 ttgctccgaa atccggggct tgctcgcggc ggacctcgcg ccccgcgaac tgagccggcg
     8161 gggcgtggaa gacttcctgc aattcggcgc gccccaggac ccggtgacca tccttcgcgg
     8221 cgtctatgcc ctgctgcccg gccacgcgct ctgctggtcg cgaggccgta cggaaatccg
     8281 gcgttatgcg gcccggccca gtccgatcca gggaggcccg gaagccatcg agccgatgct
     8341 ccggaaaatc gtccgccaac agctcatctc cgatgtcccg gcggtggtgt tcttgagcgg
     8401 cggggcggac tccagcgtca tagctctttt agcgcgcgag gaagcgggag cggacatgca
     8461 tacgatgaca gtcattttcg acgaaaacag ttattccgag cgcgatttcg cccggcgcgt
     8521 cgctgctcat gcgggcacgc gccaccacga ggttccgctc agcgccggcg acgcctgttg
     8581 ccaggcgctc gaggcgatct cccgcatgga tcagccgtcg gtggatggcg tgaacacata
     8641 tgtcatcagc gccgctgcaa aacgcagcgg cttcaccgtg gccttgagcg gcgtcggcgg
     8701 cgatgaattc ttcgggggct acagcacgtt ccgccggacg gcgctgctcg aaaagctggg
     8761 acggatagcc gggcggatgc cggctctggc gggttttgcc agccatgtcc tcaccagaca
     8821 cggcacagcc ggcgctcaaa aacttggcgt ctatttgacc caaccttcgc agcacccgta
     8881 cttcttgcag cggacgttgt ttttccccgc tcagatcggg gagatctgcg cttgcggagc
     8941 acgcgagacc gaagccgatt gcctcgccgg agaacggctg caaaatatcc tcgacgaggc
     9001 cggcgccctg gaccggctga accaggtatc cctgctcgaa gccaggacgt atatggccaa
     9061 taccctgttg cgcgacagcg atcaaatgag tatggcgcat tcgctcgaat tgcgggttcc
     9121 attcgccgat catttgcttg ccgcgttcct tttgggaata cccggcgaaa aaaaaggatt
     9181 ccatcgcgcc ggcaagcttt ggctgcgcca ggccttcggc aatcgtctgc cgccagaggt
     9241 tttcgagagg gaaaaaatgg gattcaccct cccgttcgcc gtctggttaa aaggaccgct
     9301 ggccaaagaa gtgagtcaaa cgctcgaccg gaatccccat cggatctggc ggcgagacgc
     9361 cgcccttcgg gtatggaatg attttctgcg cgggcgagcg ggatggtcgc gcccgtgggc
     9421 gctctacgtc ctcagccgct gggcggagaa aacccttcct gtgtgagcct atgcgaatgt
     9481 cgtccccggg ggacccagga cgggaatgaa tcgggcgatt aggcccattt cttcccgagg
     9541 gcctcgagcc ccgcgcggcc cagatcgtcc gccgtcatgg ccacacgcaa ctcgatatcc
     9601 gcatcaaaca gcagaaacca gggttccgct aacgccggaa tctgcgacgg atcgttcacg
     9661 ttgacaatca aggtggcccc gcggtggcca tcctgttcgg taaagtagac aacctccggc
     9721 ttgacttcat cgagaacgcg ccggattttg cttcccgcgc tgccgtccct tatcgccgtg
     9781 ttgaacggtt cgtgggggaa tttcacgttc atgagcattc gcatagggcc tccaggaagg
     9841 ggatcacggc tgattctatc gcgaaaagca tcgctcgcct gttcttttct gccttggctt
     9901 tttcctgggc ctggcctgtg ctgctcctgt tctccggcca ctccctgtct gccccgcctg
     9961 tggtagaaat agaggttcaa cgcggagggg gcgcgcttct atgacacggc ggctcaccac
    10021 ggcccaagcg atcattgaat tcctgaaaca tcagtacgtt gagcgggacg gcacggaacg
    10081 gcagttcttt ggcggatgct tcggcatttt cggccatggc aacgtcgccg gcatcggcca
    10141 ggccttgcag cagaatcccg attttcgctt ttatctgtgc cgcaacgaac aagcgatggt
    10201 gcacacagcc gccgccttcg ccaaaatgaa caaccggctg cggacgctgg tctgcaccac
    10261 gtcgatcggc cccggcgcca cgaacatgat cacgggtgcg gccgcagcga cggtgaaccg
    10321 cattcccgtg ctgctgctcc cgggcgatat cttcgcgcgc cgcaacgtgg cgcccgtttt
    10381 gcagcagctc gagtgctccg cctctcaaga catttcggtc aatgattgtt tcaaaccggt
    10441 ttcccgttat tgggatcgca tccagcgccc ggagcagatc ataaccgccc tgcccgaggt
    10501 catgcgcgtg ctgacgtctc cggccgacgc cggcgcctgc acgctcgcct tgccccagga
    10561 cgtccaggcc gaggcgttcg acttccccgc ccggctgttc gaaaaacgcg tctggcgcat
    10621 ccagcgcccg cggggcgatg cggaaacgct gcgtcgcgcg gcccagtgga tccgctcctc
    10681 aaaacagccg ctcatcattg ccggcggcgg tgtgacttac tcggaagcca ccgacgccct
    10741 ctcgcgtttc gccgcccaga ccggcattcc agtagcagaa acgcaggccg gcaagggctc
    10801 gctctgccac gaccacggcc aggcgttggg ggccatgggc gtcaccggca cgcccggcgc
    10861 aaacatcgtc gcccgcgaag ccgacctggt gatcggtatc ggcacgcggt acagcgattt
    10921 caccagcgct tccaaaaccg cgttccagaa tcccgatgtt cgcttcatca atatcaacgt
    10981 gtgcgagttt gacgcgtaca agcattgcgc gctgcccgtg atcgctgacg cccgtgcggc
    11041 gctagccgag ctcggccagg cgctcgaagg ctaccgcgtt tccgcagact accgcgcccg
    11101 tatcgaccgg ctgcgcgctg agtgggaaaa ggaagtcgat cgcatttaca gcgagcggca
    11161 tggcccgccg gtgagtcagg gggcagtgat cggcgtggtc aaccggatct ccggaccccg
    11221 ggacgtggta gtcagcgcgg ccggaagcct tcccggcgat ctccacaagc tctggcgcac
    11281 gcgcgacccc aaaggctatc acctcgaata cggctattcg accatgggct acgagatcgc
    11341 cggcggcttg ggcgtcaaga tggccgaccc cagccgcgaa gtctacgtgc tggtcggcga
    11401 cggctcctac ctcatgatgg cgcaggaaat cgtcacttcc atccaagaag gctacaagct
    11461 gaacatcgtg gtgctcgaca atcacggctt ttcgagcatc ggcggcttga gccaggcctg
    11521 cggcagcggc ggtttcggca ccgagtaccg gtatcgcgta gatggatcga gcggcgcgtc
    11581 cctcagcggc gagcccatcc cggtggattt cgccgccaac gccgcgagcc tcggcgccca
    11641 cgcggttcgg gcgcaaacta cggatcagtt ggccgaagaa ttggagaccg cgcggcgccg
    11701 cgaccggacc accgtcgtcg tgatcgaaac cgatatcaac gcccgcgtcc ccggctatga
    11761 atcctggtgg gacgttccga tcgccgaggt ttctgaaatg gaatccgtca aggccgcccg
    11821 cgccgcttac gagccggcca gacgcaagga gcggtattac ttatgacgcc ctcaccctgg
    11881 gttttccgct gccggccggc gctccgcctt gggcttcgcc ccgccgcatc gccctcttgt
    11941 aacagggaat cctgatggaa gtcaccgtcc ttggccgcgt cggttacgac ctctattctg
    12001 aagagccgca cacgccgctg ccgcaggcgc ggcggttttc ccgctatctg ggcgggtcga
    12061 gcgccaatat ggccgtcggg ttgtcccgcc tgggcgcgga ggtcggcatt gtcgccgcac
    12121 tgggcgatga cgcactaagc gatttcttgc gggattttct gcgcgccgag cgggtggatg
    12181 tttcccgggt gaagactgtt ccaggctatc tcccgtcgct ttgcctgacc gaagtttcac
    12241 cgcccgatcg cttcccgcag gtcttctatc gccgcgatcc cgccgacacc cagctcgaca
    12301 tcgacgccgg cgacctggct tatatcgcct ccgggcgcat gttcatcacc aacggaacgt
    12361 ctttatgcgc ttcgccctcg cgcgaatcca cctatcgcgc tatggagcat gcccgcgcgg
    12421 cgggctgccg cgtggtgctg gatgtcgatt accgggcaat gtcatggacc aggccggagc
    12481 acgccgggct ggccattcgc ctggcgctgc ctttcgtgga tgtgcttatc ggcaacgatc
    12541 tcgagctgaa gctggtcacc ggcgcttccg aactcgacga tgcagtagcg gccctgcgaa
    12601 aatcccggtt gcccatgctc gtatccaaac tcggatcggc gggcacccgc gtgtattgcg
    12661 gagattcctc ggtgttcctc gccccctatt cggtcgaggt ggtctccacc ataggcgctg
    12721 gagacggttt cgcctcgggc ttcttatatg cacttctaag aggtatgccg gtcgaagaat
    12781 gcctgcacta cggcaacgcg gcggccgcga tcgtagtcag ccgcctgagc tgctccgagg
    12841 ccatgcccac gctggccgaa gtcgagcagc tcattgaaga acagaaggcc cggctgacct
    12901 tgcaggctta agcctcggct cggggaactg attccgacga gaacggttcc ttggcttcca
    12961 tcgcaggcgt caggctcagc gatttgcgaa tccgcggaag ttggaaaacg cgaaccctgg
    13021 ccccacagcg gcccaggggg gttctcattg cctggtgaac gcagcggcga gccgcgtaaa
    13081 gcgggggttg cccgttccga tactcaagat taagcgcacc gcccgaattt gacgttcccc
    13141 ggtccatcag gtattctggt tacttcgata gaggcgcggc gatcatgagt aaccgcgggc
    13201 gcgttccggc cggaaccccg ggcccgcggc gaaagggata gcagggagcc gccgaagctg
    13261 cttgagttgt ccggcctcaa gcggttgggg gaacgggcta atacccttcc actgtcaccg
    13321 aacgccagac aggaccggtg gagcgctatc ggagaagacg gctgtatagc cggcttccct
    13381 gttcgccctt ccctccttcg cgttcccaat catgcacctc aatgaactca cccgcaccct
    13441 gatcgatatt gagtcgatca cggagaatga gaaggccgtc ggcgactttc tctacgaata
    13501 tctgcgccct ctggccgcgc gctttggcgg cgaactggaa cgcatggagg tggagcccgg
    13561 ccgttttaac gtcttcgcct gctggggcaa gccggagatt gtgctttcga cgcacatgga
    13621 tactgtgcct cccttcatcc gttcgcgcga ggacgatgag ttcatctggg ggcgcggcgc
    13681 ttgcgacgcc aagggaatcg ccgcttccat gctgcatgcg gcggaggcgt tgttggccga
    13741 agggcggcgg ggcttgggag tcctttttct ggtcggtgag gagcgcaata gcgcgggcgc
    13801 acgggcggcg aaccggcagc cgcgcggcgc ccgctgtctc atcaacggag agccgacgga
    13861 aaacaagctt gccctcggat cgaaaggcgc gcttcgcctg gaagtggaag cgcacggcaa
    13921 aatggcccat tccgcatacg ccgagctcgg cgattccgcg atattgaagc tgctggatgc
    13981 attggacgct ttgcgccata ttgaatggcc gtccgacccc gtactcggag agagcacgtg
    14041 caacatcgga acgatctcgg gcgggcgcgc ccccaatgtg gttcctgatt tcgcccgggc
    14101 ggaaattatg atccggctgg tgaataattc cggcgagctt tgccggaagg tcatcgccgc
    14161 cctggggcat cgggtggaga cgcgggttgt tttggaagtc ccgccggtgc ggctgggttc
    14221 tgttcccggc atcgagacga ccgtagtgaa gtttacgacc gatatccccg agctgacccg
    14281 ctggggacag ccctttctgc ttggacccgg cacgattcac gtggcgcata ccgcggagga
    14341 gcgcgtcccc aaacggcagc tcgttgaggc ggtcggcctc tatcagaaga tcgtaaagga
    14401 gctgcaagac aagccgccga atgccacccg cggcgcatag aaattatgga aacgaaaatc
    14461 gaagtaggcg tactcggcgc caccggcatg gtgggccagc attttttgag atttctcgac
    14521 ggtcacccgt ggtttcgcct cacctggctc ggcgcaagcg agcgctccgc cggcaagcgc
    14581 tactcggatg cagccccatg gcacctgaac ggcggctgcc cggagagcat cgccgccctg
    14641 agcgtcgagg agtgcaagcc gggccgcgcg ccgcggcttg ttttctcggc tatggacgcc
    14701 tcggtggccg gggaaatcga gcaggagttc gcccgcgccg gccacgttgt ggtctccaac
    14761 tcgcgcaatc accgcatgct gcccgacgtg cctctgctgg ttccggaaat caacgccgac
    14821 cacctccagc tcatcccctt gcagcagcgc gcccgcgggt ggaagggcat gatcgtcacc
    14881 aatccgaatt gctccaccgt ggctctcacg atggcgctcg cgcccctcaa acaatttggc
    14941 atcacgaaag taatcgctac aacgctccag gcaatctcgg gcgccggcta cccgggtgtt
    15001 ccctccatgg acatcatggg caacgtaatt cctcacatcg gcggcgaaga ggagaagatg
    15061 gaaactgaga cgcaaaagat tctgggagac tatgtcgccg gcgcgggaca aatccggccg
    15121 cttgcggcag cggtcagcgc gcactgcaac cgcgtcccgg tcgtcgatgg ccataccgtg
    15181 accgtgtcgg cggctttgga acagaaaccg gcgctcgaag acctgctggc cgccttcgag
    15241 agcttccgcg gcgttccgca ggaacggaaa ctgcccagcg ctccggccag accggtggtg
    15301 ctgatgcgcg aggtcaaccg cccgcagccc cgcaaggacg ccgagcgcga gcgaggcatg
    15361 gcgtgctttg tcggccgagt ccgcaagtgc cctgtgctgg actacaagtt ggtcgccctc
    15421 gggcataaca ccattcgcgg cgcggccgga gcagccgtgc tcaacgcaga actgatgtat
    15481 tcggagggcc tgctggatta gacaatcgcc ggccgcaggc gcccggtcga ggcgggtccg
    15541 cccggcgggg cgcctgcgcg gccaggtccg taacatcatc tatgatcgtc atgaaatttg
    15601 gaggcacctc ggttgagagc gcctccgcca ttgaacgcgc tgcctgcatc gtgcgcagcc
    15661 gcctggagcg caaaccggtg gtcgtggttt ccgccatggg caagacgacg aacaagctgc
    15721 tggccatcgc ccgcacggct gtcgacggca ataaaagaga agcgattcag ctcattcacg
    15781 aactgcgcga cttccattcc cacgaagcgc gtcaggtcgt ctcgctggcc gagcgggccg
    15841 agcttgactt gatcctggac gagcatttcc aggagctgac cgagctgatc aaggggctct
    15901 ccgtgctggg cgaggcgact ccccggtcga ttgacgccat ttcgagttac ggcgagcgcc
    15961 tttccagcca catcgtcgcc cgcgcgctcc gccacttcgg gctggacgcc gtgcacctgg
    16021 attcccgcag ctacgtcatc actgacaacc gcttcaatca ggcggcgccg ttgtacggcg
    16081 cgacctaccc gagactagcg gcggccctcc cgcctctgct cgagcagggc aaggttgtcg
    16141 tgatgggcgg ttttatcggc gccactgagc agggcgtgac caccacgctc ggccgcgggg
    16201 gaagcgattt ctcggcttcc atcatcggcg ccggcatcgg agccgacgag attcagatct
    16261 ggaccgatgt ggatggaatg ctcaccgccg acccgacgat cttgcctggc ggccgccggg
    16321 tgagagtcat atcgttcaat gaggcggcgg aactggccta cttcggcgcc aaagtgctgc
    16381 atccgagcac gctggttcca gccatgaata tgaatattcc ggtctttatt ctcaactccc
    16441 gccgcccgga gtgcgaaggg accaagatca ttcctcaggc cccgcccacc tcgaacctcg
    16501 ccaaatccat cgcctgcaaa aagaacctca ccgtggtgga cgtgtcttcc acccgcatgt
    16561 tgatggcgca tggattcctc cataagatct tcgaaatttt cgaccgttac gaaacccccg
    16621 tggatatggt caccacctcc gaggtcagcg tgtcgctgac aatcgacaag actgaccgcc
    16681 tggaggagat cgcccgggag ctgagccgtt tcgccgaagt aaaccacgag aatcaccagg
    16741 ccatcatttg cgtggtgggc gacaacatcc ggtatactcc gggagtggcc gggcgcatct
    16801 tcaaagccgt cgagcacgta aatgtgcgca tgatctcgca aggcgcatcg ctgcggaacg
    16861 tcagcttcgt cgtgcaagaa agcgatttga agccggcggt ggaggctctg cataaagcct
    16921 tcttctccga actcgatccg gaggtgttcg attgagcgcg ggaacttcgc cgcagccaaa
    16981 gctggcgatt gtcggttatg gcaggatggg aaaactgatc gagcagttgt cgcgagagtt
    17041 tggctttcag gttgcgctga agctggacat tgataacaat gtcaatggct ccgggatcac
    17101 gcgggagaat ttccgggaga ttcaggttgc cgtcgagttc tcgacgcctt tggtcgtagt
    17161 ggaaaatatc gagcgcattt cagctctcgg cgtaaacctt gtggtcggaa ccacgggatg
    17221 gttcgatcag atcgagcgtg tccggacggc agtggcccgt gcgggaacgg gcttggttta
    17281 cagcccgaac ttttccgtcg gcatgcagat cttttcgagg ctggtggaag ccgccgcccg
    17341 gtgctttcaa gcgcagccgg actatgacgc ctggggatac gagattcacc accgcatgaa
    17401 aaaggatgcg ccttccggca cgctgctgaa gttgaaagag gtgatggaaa aggcagggta
    17461 ttcgcgtccg attgacctcg cgtcgaaccg ggcgggcgcg attccaggca ctcacgtgat
    17521 tggcttcgat tccgaggccg atacgatcac gctggcgcac acggcccgga gccgcttggg
    17581 ttttgcgcgc ggggcgatga aggcggcgca atgggtcatg ggcaaaaaag gggtgtacga
    17641 gtttagcgag gtgctgttcg gctgagcggc cggaccatcc ggccggctgc gggcgccggc
    17701 tgggcggtga gcgccgcggc aggtttggtt ggcggttttt cgcagttgcg agctgtgagg
    17761 ctgttatgag atttcgaggt tgcggtacgg cgctggtgac tccgttccgg cgcgattt
//
LOCUS       DASREK010000148         8573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2054993, whole genome shotgun sequence.
ACCESSION   DASREK010000148 DASREK010000000
VERSION     DASREK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2054993"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /gene="amrB"
                     /locus_tag="VFA54_07395"
     CDS             <1..623
                     /gene="amrB"
                     /locus_tag="VFA54_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZS50667.1"
                     /translation="LPERFIILCPNHTGRGGSLAIMGEGSWQTPLGDAPVDSALAARI
                     MKHCPLLSEDSEAHEEEHSLEVQLPFLQFLKPGFSFVPIAIGLDRFADLEFLGHSLAR
                     AVQESEPRPLIIASSDMNHFETDERTRIKDRRAIDRILALDPRGLYDTVRRDSISMCG
                     YAPATAMLTAALDLGAQRAELVRYGTSADVTGDTSRVVGYAGIAVW"
     gene            complement(724..1188)
                     /locus_tag="VFA54_07400"
     CDS             complement(724..1188)
                     /locus_tag="VFA54_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZS50668.1"
                     /translation="MPGHTFQLYREQLVRRPLREVFRFFTEAKNLELITPDFLHFRIL
                     TPPALLRIEPGARIDYKLRLFGIPFHWCTQIETLEPERRFTDVQLAGPYASWHHLHEF
                     RETEGGTLVIDNVEYELPLGLLGRMAHALFIRRSLERIFEHRRQRVEELLNR"
     gene            complement(1191..2093)
                     /locus_tag="VFA54_07405"
     CDS             complement(1191..2093)
                     /locus_tag="VFA54_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HZS50669.1"
                     /translation="MRALVTGATGFIGRRLLSELERPVVLTRDPGRAQSLLGAGVQAF
                     PWEPEAGPPPPDCFRDVDVVFHLAGESVGEGRWTAEKKKRIMSSRVRGTRHLVSGIEA
                     AHPRPAALVSASGVGYYGDRGDEILDESAQPGGDFLAQVCQAWEAEAARARSLGLRVV
                     APRIGLVLGKGGGALARMLPPFKLGLGGRLGSGRQWVSWIHLDDLAGLLLYAARKPEI
                     NGPMNAVAPGAVTNREFTRVLAAVLKRPAIFSVPAPLLRLALGEFASVLLASQRITPR
                     IALETGYQFRYPALEDALRSAAGN"
     gene            complement(2135..3364)
                     /gene="amrB"
                     /locus_tag="VFA54_07410"
     CDS             complement(2135..3364)
                     /gene="amrB"
                     /locus_tag="VFA54_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HZS50670.1"
                     /translation="MQSLPKLRTDLEFMPSPLEDRPGLFIRDPYRYTDAAVIVPPLLA
                     EGLAFFDGQQTELDLQAYFTRRTGQLVPSDVVRSMVHALSEQGFLHTEKFDRMKLDKQ
                     RAFWEASQRMPAHAGSAYPAEAAGLQQRLAEFTRDGAGQSQNGFPAGSRRIALAAPHV
                     SPEGGWRSYAAAYAGLGPELADKTFVILGTSHYGEPQRFGLTRKPFVTPLGTLEPDLP
                     LVDRLAARGGDAVALEDYCHSIEHSIEFQCIFLQHQLGVRIKILPILCGPFIESLLTG
                     RAPETNESVRRFFNELREIEEAYGNRIFWILGIDLAHIGRRYGDAFAARAQEGRMVEI
                     QRADEERLEKVCSGESEAFFELVRPGHDELRWCGYSPLYTFLQAAPAARGAVLRYEQW
                     NIDEMSVVSFAALEFWK"
     gene            complement(3560..3994)
                     /locus_tag="VFA54_07415"
     CDS             complement(3560..3994)
                     /locus_tag="VFA54_07415"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZS50671.1"
                     /translation="MADLEIRPTMRFIAIRYAITLILLIAALFWWGVSPDETLRLAAV
                     AGAAALLLWPLGLHWKRQRIRCLLDGGQLRYESGLVTTSVKSLPVANIQDVTVRTTLV
                     QKLFGVGDLRIETAGQGSGFEIANVEKPHKAAEIILAAAARR"
     gene            complement(4061..4708)
                     /locus_tag="VFA54_07420"
     CDS             complement(4061..4708)
                     /locus_tag="VFA54_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50672.1"
                     /translation="MKGLAGACGLILLLGTTLIAQNRDFLTDDEISQIREAQEPNERL
                     TLYLKFARLRLELLKQAMAVEKPGRAKLIHDNLEDYTHIIEAIDSVIDDALLRKADLT
                     KTIGTLAAREKEFVATLEGFAAQRPKDRYLYDFALKDALETTQDSLDESQQDLKERAH
                     RLAVEDAGEKKERESMMTPAEQESKKAEEKKAGDKAKKSPKAPTLRRKGEAPDQP"
     gene            complement(4716..5156)
                     /locus_tag="VFA54_07425"
     CDS             complement(4716..5156)
                     /locus_tag="VFA54_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50673.1"
                     /translation="MMVPSLMLMLALAQGNPPENLQAIAAEPNLVKRARLALQRGDRD
                     TNQAGQACKDGDYDSCNAELNHVREDVELADRSLKETGIDPARSPRHFKEAELHIRKI
                     LRSIEAVRAYLRPEDLDHFDTIYQRISEIDDRLVAAILSRKKKK"
     gene            complement(5167..6420)
                     /locus_tag="VFA54_07430"
     CDS             complement(5167..6420)
                     /locus_tag="VFA54_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50674.1"
                     /translation="MLWPLLVILAIQAASSGRAAAPPQALDAAAAALEAGKYAEAAEL
                     LEKAVAADPKDYRARFNLAFAYTQLHRETEAIAQYTKVVEQQPDLAPARLNLGILLLR
                     QEKASAAVPHLELAAEKRLQDFRAQFYLGEALLKAGLSGRAAAAYQRALAIDPKSAAA
                     VLGLARSFARQGQIDSAREQYLRAAQMDRQFNDALLELGDLLEGRKQPDEALGLYLEY
                     LKTKPEAVAVQERAGVLLLQSKRYPEAIEHLEAAVRQNPTAANQTALAQAYTMSRQPA
                     KAVPLLRAAVIAEPDDLDLHLRLAMALLETMDYGGASKEFAAVAQKQPSNVDAWNGLG
                     FTLYKVENYDGALQALERARQLGSEPPGNEFLRAIILDRFHQYPAALESYQKFLAASG
                     GKDPDDEFKARQRVRIITNMLNKRR"
     gene            complement(6425..>8573)
                     /gene="uvrA"
                     /locus_tag="VFA54_07435"
     CDS             complement(6425..>8573)
                     /gene="uvrA"
                     /locus_tag="VFA54_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZS50675.1"
                     /translation="GEAIFESAEDPSRQLRFTERFECKRCRLEYQEPEPRLFSFNSPF
                     GACPRCQGFGNVMDYDMERVIPDPTLTLAEGAVEPWTKPSYYRFFLDLRRSAASKKLR
                     LDVPYRELSRREQEIVLEAVRRFFARLEQKKYKMHVRVFLSRYRGYGPCPECKGARLR
                     KEALQVRLGGKNIAEVVHMSIEQAKPFFDGLELSPQEMALADKVLHEIRQRLAFLNDV
                     GLEYLTLDRLASTLSGGEAQRIQLATSLGSRLVGACYVLDEPSIGLHSRDTQRLIGIL
                     RELRDLGNTIIVVEHDRDVMRSADFIVDLGPGAGENGGQAVFAGPYAKLVANGGGSLT
                     GRYLTGEMSIPVPGVRRRIDRKQQLRFLGAYAHNLKSIDVAIPLHALVVVTGVSGSGK
                     STLLHDVVYQGLMAVHGPLGPQPDMRDEGLDFLEDAGGDYPRLQCRRIEGAELIEEVV
                     LVDQSPIGRTPRSNPITYIKGFDLIRELFASTPDARKRGYSAGHFSFNIAGGRCETCQ
                     GDGTVTVEMQFLADVELVCEDCKGKRYKSSILDIHFQGRNIYDVLQMTVREALAFFAA
                     YPRITSKLKLLADIGLGYLRLGQSATTLSGGEAQRVKLAAHLARARCDRTLYIFDEPT
                     TGLHFDDIAKLLGAFRRLIESGGSVLVIEHNLEVIKTADWVIDLGPEGGERGGRIVVE
                     GPPERVAACAQSYTGRFLREALHLSPAEISAIQ"
BASE COUNT         1627 a         2748 c         2509 g         1689 t
ORIGIN      
        1 agttgcccga gcggtttatc atcctttgcc ccaaccacac gggccgcggc ggctctctgg
       61 cgatcatggg ggaaggatcc tggcaaactc cgctcggcga cgcgccggtc gacagcgccc
      121 tcgccgcccg gatcatgaag cattgcccgc tgctctccga ggattccgag gcgcatgaag
      181 aagagcactc cctcgaggtc caactgccct ttttgcagtt tctaaaacct ggattctcat
      241 ttgtcccgat tgccataggg ctggaccgat ttgcagatct ggaattcttg ggacacagcc
      301 tggcccgggc ggttcaggag agtgagccgc ggccgctgat catcgcctcc agcgacatga
      361 atcacttcga aaccgacgag cgcacgagga tcaaagaccg ccgcgctatt gaccggatcc
      421 tggctctgga tccccgcggg ctgtatgaca cggttcgccg cgacagcatc agtatgtgcg
      481 gctacgcccc cgccacggcg atgctcaccg ctgcgctcga tctgggagcg cagcgcgccg
      541 aactggtccg ctatggtacg agcgcagatg ttaccggaga caccagccgg gtggtcggct
      601 acgccggcat agccgtctgg tagtgatgtg cggtcccgct tccgccattg ctgacgccgg
      661 aagctctgat atctccttgg gactttcaga tacgaacgcc ggtccgcgct ttccggccct
      721 tggtcaacgg ttcaacagct cctcgacccg ctggcgccgg tgttcaaaaa tgcgctccag
      781 cgaacgccgt atgaagagag catgcgccat acgcccgagc aggccgagcg gcaactcata
      841 ttccacgttg tctatcacca gagtgccgcc ctccgtttcg cgaaattcat gcaaatgatg
      901 ccagcttgcg tacgggccgg ccagctgcac gtcggtgaag cggcgttccg gctcgagcgt
      961 ctcgatttgg gtacaccagt ggaatggaat gccgaacagc ctcagcttgt aatcaattcg
     1021 cgcacccggt tcgatgcgaa gaagagctgg cggcgtcaga atgcgaaagt gcaggaaatc
     1081 cggcgtaatc agttccaggt ttttcgcttc ggtgaagaac cggaagacct cccgcagagg
     1141 ccttcgaacg agctgctccc gatagagttg gaaggtatgt ccgggcacgc tcagttgccg
     1201 gccgcactac gcagcgcgtc ctccagggcg gggtatcgga actggtagcc cgtttccagc
     1261 gcgatgcgcg gcgtgatgcg ctgggaggcc agcagcacgg aggcgaactc gcctaaagcg
     1321 aggcgcagca gaggcgcggg aacagagaaa atagccggcc gcttcaatac cgccgcaagc
     1381 acccgggtaa actcgcgatt cgtgacagcg ccgggcgcca cggcgttcat aggcccgttt
     1441 atttctggtt tccgggcagc gtacaaaagc aggccggcaa gatcgtcgag atggatccac
     1501 gagacccatt gccgcccgct tcccagccgg ccgcccaacc caagtttaaa gggaggcagc
     1561 atgcgcgcca gagccccgcc acccttgccg agaaccaagc cgattcgcgg agccacgacc
     1621 cgcaagccca gacttcgcgc ccgggcggct tccgcttccc aggcctggca aacttgagcc
     1681 aaaaaatcgc ctcccggctg cgcggattca tccagaattt cgtcaccccg atcgccgtaa
     1741 taaccgacac ccgaggcgga aaccagagcc gcaggacgcg ggtgcgcagc ttcgatgccg
     1801 gataccaaat ggcgagtgcc gcggacacgg ctgctcatga tccgcttttt cttttccgcc
     1861 gtccatcgcc cttcgcctac ggattccccc gccagatgga aaactacgtc gacgtctcga
     1921 aagcaatccg ggggcggcgg accggcttcc ggctcccagg ggaaagcctg cacgcccgcg
     1981 ccgagcaggg attgcgcccg acccggatcg cgggtaagca caaccgggcg ctccagttcg
     2041 gagagcagcc tgcgtccgat aaacccggta gctccggtga ctaacgcccg catcacggct
     2101 cctgttcaat ggcgcgcggc ctttcccgcg cgccttactt ccagaattcg agagctgcga
     2161 agctcaccac cgacatttca tcgatgttcc actgctcata gcgcagcacc gcgccgcgcg
     2221 ctgccggcgc cgcttgtaga aaagtgtaaa gaggcgagta gccgcaccac cgtaattcgt
     2281 cgtgcccggg cctgaccagc tcaaaaaatg cctcgctttc tcccgaacag accttttcta
     2341 gccgctcctc gtcggcgcgc tggatttcga ccatacggcc ttcctgcgcg cgagcggcaa
     2401 aagcatcccc gtagcggcgg ccgatgtgcg ccaggtcgat ccccaggatc caaaaaatgc
     2461 ggttgccgta agcttcctcg atttctcgca attcgttgaa aaagcggcgg acagactcgt
     2521 tggtttccgg cgcgcgcccg gtgaggaggc tttcgatgaa cggcccgcaa agaatcggta
     2581 aaattttgat ccggactccg agctgatgtt gtaaaaaaat acactgaaac tcgatcgagt
     2641 gctctatgga gtgacaatag tcttcaagag ccaccgcatc cccgccgcgg gcagccaggc
     2701 ggtctaccaa aggcaaatcc ggctcgagcg ttccgagcgg ggtcacgaaa ggctttcgcg
     2761 tcagcccgaa tctctgcggt tcgccgtagt gggatgtgcc cagtatgacg aacgtcttat
     2821 ccgccaactc cggccctaac cccgcgtaag ccgccgcata actgcgccat ccgccctccg
     2881 gactgacatg gggcgccgcc aaagcgatcc tccggctgcc cgcgggaaat ccgttctgac
     2941 tctggccggc gccatcccgc gtgaattccg ccagccgctg ttggaggccc gccgcctcag
     3001 ccggataggc cgaaccggca tgcgcaggca tccgctgtga ggcctcccaa aaggcgcgtt
     3061 gcttatccag cttcatccga tcaaactttt ccgtgtgcag aaaaccctgc tccgacaacg
     3121 cgtgcaccat gctgcggaca acatcggaag gaaccagttg accggtgcgc cgcgtgaaat
     3181 acgcctgaag atccagttcc gtttgctgtc cgtcgaaaaa cgccaggccc tcggcgagca
     3241 gcggcggcac gatcacagcc gcatcggtgt agcggtaggg atcgcgaatg aacagtccgg
     3301 gccggtcttc gagcggagaa ggcatgaatt cgaggtccgt tctcaacttg ggcagagatt
     3361 gcataaagtt tagctcaaaa acactccctt ctgagagggc gggaacggct gcggccgcgt
     3421 tgcatttcgg ctgcgaatgt ccgcacctct gcgcggaaac atccgcctgc ttggatgcgc
     3481 gccagccggc cccggattcg aaaaggaggc aacttcacgg ccggaaggcc tccggagaaa
     3541 caagctgtgt gctcatcctt cagcgtcttg cggctgctgc caggatgatt tccgccgcct
     3601 tgtgtggttt ttccacattg gcgatctcga agccgcttcc ctgaccggcc gtttcaatcc
     3661 gcagatcccc gactccgaat agcttttgca ccagagtagt ccgaaccgtc acgtcttgaa
     3721 tgttggccac gggcagactt ttcaccgaag tggtgaccag tccggattca taccgcagtt
     3781 gtccgccgtc gagaaggcac cggatgcgct gtcgcttcca atgcaagccg aggggccata
     3841 acagcagggc tgctgcgccc gccaccgcag ccaggcggag ggtttcgtcg gggctgacgc
     3901 cccaccagaa cagcgcggcg atcaacaaaa tcagagtgat ggcgtagcga atcgcgatga
     3961 aacgcatcgt cgggcggatc tcgaggtctg ccatttgcgg cggctcctgt cggactcgta
     4021 aggaatcata ctgcgccaag cggcaaaccc ccaagcgttt ttacggctga tcgggcgcct
     4081 cgccctttcg gcgcagcgtc ggcgctttag gactcttttt cgccttgtct ccggccttct
     4141 tctcttccgc ttttttgctc tcttgctcgg cgggcgtcat catcgactcg cgctctttct
     4201 tctcaccggc gtcttcgacg gccaggcgat gagctctttc tttgaggtcc tgctgtgatt
     4261 catccaaact gtcttgagta gtctccagag catccttcaa ggcgaaatcg tagagatagc
     4321 gatccttcgg gcgctgcgcg gcaaatccct cgagggtggc gacgaattcc ttttcccggg
     4381 cggccagcgt tcctatggtt ttggtcagat ccgcttttcg cagcaaagcg tcgtcgatca
     4441 ctgaatcgat cgcctcgatg atgtgcgtat agtcctcgag attgtcgtga atcagtttgg
     4501 cgcggcccgg tttctcgaca gccatcgcct gtttcagcag ctcaagccgg agcctcgcga
     4561 atttcaggta aagagtgagg cgttcattcg gctcctgcgc ctcacgaatc tggctgattt
     4621 cgtcgtcggt cagaaaatcg cggttctggg cgatcagcgt tgttcccaac aacagaataa
     4681 gcccgcaggc tcccgctagg cctttcatat gcgcctcact ttttcttctt cctgctcagg
     4741 atggccgcga ccaggcggtc atcgatttcc gagatccgct ggtagatcgt atcaaagtga
     4801 tcgaggtctt caggccgcag ataagcccgg accgcctcaa tactacgcag aatctttcgg
     4861 atgtgcagtt ccgcctcttt aaagtgccgc gggcttcgcg ccggatcaat gcccgtttcc
     4921 ttcagggacc tgtcggccag ttccacatcc tcccggacgt gattcagttc ggcgttacag
     4981 gaatcgtaat cgccgtcctt gcaagcctgg ccggcttgat ttgtgtcgcg gtcgcccctc
     5041 tgaagcgcga gccgagctcg cttgaccagg ttcggctcgg cggcaatcgc ctggagattc
     5101 tctggaggat ttccctgggc taacgccagc atcagcatca agctgggaac catcatgccg
     5161 ccgcacctat cgacgcttgt tcagcatatt ggtgatgatt cgcacgcgct gccgcgcctt
     5221 gaactcatcg tcgggatctt tgcccccgga agccgcgaga aacttctggt agctctcaag
     5281 agcagccggg tactggtgaa atctatccag gataatggcg cgcaaaaact cgtttccggg
     5341 cggctcagag ccaagctggc gcgctctttc cagcgcctgc aaagccccgt cgtagttctc
     5401 aaccttgtat agcgtgaagc ccagtccgtt ccaggcgtca acgttactag gctgtttctg
     5461 ggcgacggcg gcaaattcct tcgacgcgcc gccgtaatcc atcgtctcga gcagggccat
     5521 ggccagccgc aagtgcagat cgaggtcgtc cggttcggcg atcaccgcag cccggagcag
     5581 cggcacggct ttggcgggct gccggctcat cgtgtaggcc tgcgccagcg ccgtctggtt
     5641 ggcggccgtc ggattctgcc gcaccgccgc ctccagatgc tcgatcgcct cgggataacg
     5701 cttcgactga agcagcagca cgccggcgcg ctcttgcacg gccaccgcct cgggcttggt
     5761 tttcagatac tccaggtaaa ggccgagcgc ctcatcgggc tgtttccggc cttccagcag
     5821 gtcgccgagc tccagaagag cgtcattgaa ttgccggtcc atctgggcgg cccgcaaata
     5881 ctgctcccga gccgaatcga tttgcccttg tcgggcgaaa ctgcgcgcca agccgagcac
     5941 cgcggccgcg gacttggggt caattgccag cgccctctgg taggctgccg ccgcgcggcc
     6001 gctcaagccc gccttgagca gcgcttctcc gagatagaat tgggcgcgaa aatcctgaag
     6061 gcgtttttca gccgctagtt ccaaatgcgg aactgctgcg gaagctttct cctgccggag
     6121 cagcaaaatc ccgagattga ggcgcgcggg cgcgaggtcc ggctgctgct cgacgacttt
     6181 tgtgtattgg gcgatggctt ccgtctctct atgtaactgt gtgtaggcga aagccaggtt
     6241 gaagcgggca cggtagtcct tcggatcggc tgcgactgcc ttttccagca gttccgccgc
     6301 ctcggcgtat ttaccggcct ccagcgccgc cgcggccgcg tccagagcct gcggaggcgc
     6361 cgccgcacgg cctgaagagg ccgcctggat tgccagaatc accaggagcg gccataacat
     6421 agctttattg tatcgcggag atttcggctg gagagagatg cagcgcttcc cgcaagaacc
     6481 gccccgtgta cgactgggcg caggccgcca cacgctccgg cgggccttct acgacgatgc
     6541 gcccgccccg ctcgccgccc tccgggccca aatcgattac ccagtcggcc gtcttgatca
     6601 cttccagatt gtgctcgatt accagcacgc tgcccccgct ttcaatgagg cggcgaaacg
     6661 ccccgagcag cttcgcgatg tcgtcaaagt gcaaaccggt cgtgggctcg tcgaagatgt
     6721 aaagcgtgcg gtcgcagcgc gcccgcgcca gatgtgcggc gagcttcact ctctgcgctt
     6781 cgcctccgga gagcgtggtg gcggattgtc ccagccggag atagcccagc ccgatgtcgg
     6841 ccagcagctt gagcttcgac gtaatccggg gataggcggc gaagaacgcc agcgcctccc
     6901 gaaccgtcat ctgcaagacg tcataaatat tgcggccttg gaaatggata tcgagaatgc
     6961 tgcttttata ccgcttgcct ttgcagtcct cgcaaaccag ctcgacgtcc gccaggaatt
     7021 gcatttccac ggtcaccgtt ccgtcgccct ggcacgtttc gcagcgcccg ccggcgatat
     7081 tgaaagaaaa gtggccggcg gaatacccgc gcttgcgggc gtcgggcgta gatgcgaata
     7141 attcccggat cagatcgaac cccttgatgt acgtgatcgg atttgatcgc ggcgtgcggc
     7201 cgatcggcga ttggtccacc agaaccactt cctcgatcag ctcggcgccc tcgatgcggc
     7261 ggcactggag cctcgggtag tccccgccgg cgtcctccag gaaatccaat ccttcatccc
     7321 tcatatccgg ttgcggcccg agcgggccat gaaccgccat cagcccctgg tacaccacgt
     7381 catgcaggag cgtagacttg ccggatccgg aaactcctgt caccacgacc agcgcgtgga
     7441 gcgggatggc gacatcgatg ctcttcagat tgtgagcgta ggcgccgagg aagcgaagct
     7501 gctgcttccg gtcaatccgc cggcggacgc ccggcacggg aatgctcatc tcccccgtca
     7561 ggtagcggcc ggtcagagat cccccgccat tcgctaccag cttggcatag gggccggcaa
     7621 aaaccgcttg accgccgttt tcgccggctc ccggcccgag atcaacgatg aaatcggccg
     7681 agcgcattac atcccggtcg tgctccacga caatgattgt gttgccgaga tcacgtagtt
     7741 cgcgcaggat tcctatcagg cgctgggtat cacggctgtg caacccgatg gaaggctcat
     7801 cgagcacata acacgcaccc acgaggcgcg atccgagcga agtggcgagt tgaatccgct
     7861 gggcctcgcc gcccgaaagc gtggaggcga gccggtccag cgtcagatac tccagcccga
     7921 catcgttcaa gaacgcaagc cgctgccgga tttcgtgcag caccttgtcg gccagcgcca
     7981 tttcttgcgg actcaactcg agcccgtcga agaacggttt cgcttgctct atgctcatat
     8041 gcacgacttc ggcgatgttt ttcccgccca ggcgcacctg aagcgcttct tttcgcagcc
     8101 gcgcgccctt gcactcggga cacggcccgt agccccggta gcggctcagg aaaacgcgca
     8161 cgtgcatttt gtatttcttt tgctcgagac gcgcaaagaa gcgccgcacc gcctccaaca
     8221 cgatctcttg ctcccgcctg ctcaattccc ggtaaggaac gtcgagccgg agctttttgc
     8281 tggccgcgct gcgtcgcaga tccaaaaaga agcggtaata cgaaggcttg gtccacggct
     8341 cgaccgcgcc ttccgccagc gtgagcgtcg gatccggtat gacgcgctcc atgtcgtaat
     8401 ccatgacatt gccgaaaccc tggcagcgcg ggcaggctcc gaatgggctg ttgaagctga
     8461 acaggcgcgg ctcgggttct tggtattcaa ggcggcagcg cttgcactcg aatctttcgg
     8521 tgaaccggag ctggcgggag gggtcttcgg ccgactcgaa gatcgcctca ccc
//
LOCUS       DASREK010000149        11003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_75844, whole genome shotgun sequence.
ACCESSION   DASREK010000149 DASREK010000000
VERSION     DASREK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75844"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VFA54_07440"
     CDS             complement(<1..986)
                     /locus_tag="VFA54_07440"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZS50676.1"
                     /translation="MPKFLITGGAGFIGSHLAERLLALGQRVSVLDNLSTGSIANLAH
                     LRSHPDFEFVMDEIGNERVVAELVDAADVVFHLAATVGVQKIIDSPVETIVNNVHGTE
                     IVLNAVAKKRRKAVIASTSEVYGKSASLPFREDGDLVLGPTSKSRWSYACSKAIDEFL
                     AIAYWREKKTPAVVARLFNTIGPRQSGQYGMVVPRFIGQALEGRDVTVYGSGEQTRSF
                     TYVEDVVTWLILLAQDDRAVGEIFNLGNPNEVAIKDLARRVIALTGSSSKLRFVPYDE
                     AYESGFEDMGRREPDIAKVVALTGYRPRVDLDEALRRTIDWFAERRLEDQQR"
     gene            complement(1108..2418)
                     /locus_tag="VFA54_07445"
     CDS             complement(1108..2418)
                     /locus_tag="VFA54_07445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS50677.1"
                     /translation="MPEPLSFCMVTTFYPPYSFGGDAIYLYRLCNELARAGHTVDVIH
                     CADSFRMFRPEPDLHTFAARPGITVHKLESGLGMLSPLLSHQTGRPLLKAGKIRDILE
                     SRRFHVIHFHNISLLGPAVLEMEPSYQDWVKLYTMHEHWLVCPMNVLWKYNRRACEKP
                     DCLSCTLVSGRPPQFWRYTGLRDRAASRVDQFLSPSRFTRDMHHRRGFPFPIEVLPYF
                     LDPADEEGISTAPAPHPRPYFLFVGRLEKIKGLQNVLPEFRGEGDYDLLVSGSGTYER
                     ELRKQARGMKRVKFLGWTSQDLIGPYYRHALGVIVPSITYETFGIIIIEAFARKTPVI
                     VHDLGALPEVVHDSGGGFVYRTLEELRGGMRSLAGQPELRRELGEKGYDAFCRFWSPE
                     PHLALYSRFIREAAQRKFGSVPWETEAEHRAETEKAAMRQGEAT"
     gene            complement(2402..3583)
                     /locus_tag="VFA54_07450"
     CDS             complement(2402..3583)
                     /locus_tag="VFA54_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZS50678.1"
                     /translation="MRIGVDAAVWDNRRGYGRHARALLAAAVELDRSNQYVFFTDSPQ
                     PPANLPPGGEIVCVSASAPAIEAARSNGRRRLRDLWAMSRALGRADLDCLLFPTVYSY
                     SPVVSRAYKIVVIHDVIPEKFPEYVFPAPANRWNWKLKSFLARRQADLILTVSEFSRR
                     EILEFFGEAPERVKVIGEAGDRAFRVIENPVLSERLREAGLRPGDRMLVFVGGFSPHK
                     NLAGLLDAFTVLLERWSDLRLVLAGDHQGDSFYSCYEQIRARASAPPLAGHVIFTGYI
                     PDEDLAHLLNLATALVLPSFQEGFGLPAIEAAACGLPVVATSASPLPELLGEGALCID
                     PFDGDALRHALGRVLADADLRRRMREHGLRAAASLSWEKAARELLAIFDQVARRHARA
                     A"
     gene            complement(4044..5441)
                     /locus_tag="VFA54_07455"
     CDS             complement(4044..5441)
                     /locus_tag="VFA54_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZS50679.1"
                     /translation="MTARELAHSIVERLRASGHAAYLAGGCVRDMLLGCEPKDYDVAT
                     DAPPARIQELFPDAIAVGAQFGVMLVRRDSIQVEIATFRSDHPYLDGRRPSGVTFTAS
                     AEQDVLRRDFTINGLLLDPLSGEILDFVGGRADLDAKLVRAIGNPSERFAEDRLRMIR
                     ALRFAARLGFRVEAGTMAAIQSQSAAIRQVAIERVRDELVRILTEGGARRGVELLEEA
                     GLLPHLLPEVSAMKGVAQPPEFHPEGDVWTHTLLMLEMMERPTVTLAMGVLLHDVGKP
                     PTFRVAERIRFDGHVEIGAAMAEEICLRLRFPRRQTDQIVALVRHHLRFKDAPRMRES
                     TLKRFMRLEAFEEHLELHRLDCLASHKNLRNYEFVLRAWKTLPPEAIRPAKLITGDDL
                     IAEGYQPGPLFRRILEAVEEAQLDNRITSRQDALAFVRAKFPALRSQASSSSSSMGEH
                     HPQQNANDAANGQCS"
     gene            complement(5438..6724)
                     /gene="dinB"
                     /locus_tag="VFA54_07460"
     CDS             complement(5438..6724)
                     /gene="dinB"
                     /locus_tag="VFA54_07460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZS50680.1"
                     /translation="MRTIFHIDMDAFFVSVEQLYDRRLKGRPVVVGGRPHERGVVAAA
                     SYEARKFGVHSAMPLRTAYRLCPQALFIEGHPDRYREHSKQVYEIFGRFSPQVEMASI
                     DEAYMDLTGCERLFGPPLRAAHLLHEAVERETHLPCSIGIGTSRLIAKIASDLAKPCG
                     VLWVVPGAERDFLAPLDVCRIPGVGRVMERRLRFEGIRTIGDLAAAQIGVLDHEWGIT
                     GTALAGKARGEDAGAWFARPVGAHEAPKSVSHETTFGRDTEDPELLEATLARLSQMAG
                     RRLREYGLYGRTITLKLRHSDFSTITRARTLDEATQLDTVIFQAAQELLRENWGQNLP
                     GSDSRRTAPPPIRLLGVQVSGLSGQPGQMSLLESERREKWRRALQAADSLRDRYGERS
                     IELAGGLRRRWRERVHENPAGLPGKNRPGPAKDRRT"
     gene            6888..7865
                     /locus_tag="VFA54_07465"
     CDS             6888..7865
                     /locus_tag="VFA54_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50681.1"
                     /translation="MRRLFLGAILAGAIAIAAGAGWWLWRQKQNERFLFRRLPGENSL
                     LLYLDMNKLRRSEALLPLVRSQVDPDPDYAAFVRQTGFDYQRDLDEAAVCYLADRVYV
                     LARGRFDEPRLSEYAAAQGGSCAGGGVSGKTRVCWMPASRPDRKISFFILSPGVLALA
                     TAPEPDAVLQLEVLPVPSAEPLAQAAAARSKGMALLWATAAPPALDRLLSETGGGVSP
                     NLLLLSRALAAAQRAYLLLMDRSPNLELSLQAVCASEAQAGELRRLLQGLNDLIGGLL
                     RGPASKGPPSDWGRLLASAVIAQNQNTVTATWTLDQSILRNFGPAQGAR"
     gene            complement(7936..8838)
                     /locus_tag="VFA54_07470"
     CDS             complement(7936..8838)
                     /locus_tag="VFA54_07470"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZS50682.1"
                     /translation="MRKNYFIVVLAHSVHGRLHRIQVPQYFLYVIMAFAVLGSITCFG
                     FVSSYARMLFKVSNYNNLRSEKEALMRRYDNLQQVVAQTNTQLASLETLASEISVAYG
                     LKRKLLGPDDIAQEAALVPSYQASLEQFNFLENVPLTSNGIGESMPWHWLENTVPSIW
                     PVQGALTGAFGSRIDPFQGEGAFHAGVDISSPYGSPVVAAADGVVASAGFANGYGRLI
                     ILQHGKTNINTYYAHLSECFVRPYQVVRRGEVIGRVGRSGRTTSAHLHYEVRRNHTPL
                     NPIPYLKAPFRDFAHLQPRLGPVL"
     gene            9006..9959
                     /gene="thiL"
                     /locus_tag="VFA54_07475"
     CDS             9006..9959
                     /gene="thiL"
                     /locus_tag="VFA54_07475"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZS50683.1"
                     /translation="MKKNELSIIQSIRRRAAKAAPQAARIRLGIGDDAAVLEALPRGE
                     ELLVTTDLLIEGSHFRRERHPARALGHKALARGLSDIAAMGGQPLYALLSVCLPPWAD
                     EKWRKQFFAGLFHLARSAGVALVGGDLSAGGRFAADIVVLGSAARGQVLQRSRAAPGE
                     LIYVSGSLGGSALGLARLRRGQCSRADPAVRRHLYPIPRLALGRFLAEKLHVQAAMDL
                     SDGLSLDLFRMAAESRVGAEIWAEALPVFPGANRGQALHGGEDYELLFTLGSGRQVPR
                     SFEGVPLTAIGVVTRSRRLSLIGPGGRRTRLPVGGFQHKLA"
     gene            10110..>11003
                     /locus_tag="VFA54_07480"
     CDS             10110..>11003
                     /locus_tag="VFA54_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS50684.1"
                     /translation="MFNADLNRRDLFRAAATGAVALTAASYSRILGANDRINLGVIGC
                     GARGLSDMRDFQKNPSVAVTAVCDIYGAQTDQALQRAPGAQGFSDHRKLLELKNVDAV
                     LIATPDHWHAGTTIDALNAGKDVYVEKPLTLTIDEGPKIVKAARVNNRICQVGMQQRS
                     GRHYLRAKQEYFATNKLGKITLARTWWHGNTYHLRKAPESLQHKPDNLDWARFLGPVK
                     WRDWDPQQYYNFRAYLDFGGGQVTDLFTHWIDVVHMFMDQEIPISATAAGGIYYYKDG
                     RTAPDTINVLLEYPGEWTATFE"
BASE COUNT         2081 a         3443 c         3455 g         2024 t
ORIGIN      
        1 tgcctctgct gatcttcgag ccgccgctcg gcgaaccaat caatggtcct ccgcaaggct
       61 tcatccaaat cgacgcgcgg gcggtagccg gtgagcgcca cgaccttcgc gatatcgggc
      121 tcccgccggc ccatatcttc aaagccgctt tcgtaggcct catcgtaggg cacgaagcgg
      181 agcttcgaag acgaaccggt cagggcgatc acgcgccggg cgaggtcttt gatggcgacc
      241 tcgttcggat tcccaaggtt aaagatctcg cccacggcgc gatcgtcttg ggccagcaga
      301 atcagccatg tgaccacgtc ctcgacgtaa gtaaagcttc gcgtctgctc gccgctgccg
      361 tagacggtca cgtccctgcc ttccagagcc tggccaatga agcgcggaac caccatcccg
      421 tactggccgc tctgccgggg gccgatcgta ttgaacaggc gtgcgactac ggccggagtc
      481 tttttctccc gccagtaggc gatggctaga aactcgtcga ttgccttgct gcaagcgtag
      541 ctccagcggg acttcgacgt gggccccagt accaggtcgc catcctcgcg gaacggcagg
      601 ctcgcgcttt tgccgtatac ttccgaggtg gatgcgatca cggccttgcg gcgttttttg
      661 gccacggcgt tcaaaacaat ctccgtcccg tggacgttat tgacgatcgt ttccaccggg
      721 ctgtcgatga tcttctgtac gcccaccgtg gcggcaaggt ggaaaaccac gtcggctgca
      781 tccaccagtt cggcgaccac ccgttcgtta ccgatttcgt ccatgacaaa ctcgaaatcc
      841 ggatgcgaac gcagatgggc cagattggcg atgctgccgg tcgagagatt atcgaggacc
      901 gagacccgct gccccaaagc aagcagccgc tcggccaaat gagacccgat gaagcctgcg
      961 cctccggtga ttagaaactt tggcatgctg cgatgtcatc cccgctttct acaaagaaac
     1021 agaagcagcc taaactgtgg aacgccgaga gccgtttctg gcgcccttcc ttccggatca
     1081 cgagcgggcg ggagagggcc tgcgccctca ggtagcctcc ccttggcgca tcgccgcttt
     1141 ctccgtttcc gcgcgatgct ccgcctccgt ttcccaaggc acgcttccaa atttgcgctg
     1201 ggccgcctcc cggataaagc gggagtagag cgccaggtgc ggttcggggg accaaaagcg
     1261 gcaaaatgca tcatagcctt tttcgcccag ttcccgccgc aactccggct gtccagccag
     1321 ggatctcatg cctcctcgca gttcctccag ggttcgataa acgaagccgc cgccgctgtc
     1381 gtgaactact tccggcagcg ccccgagatc atggacgatc accggcgttt ttcttgcaaa
     1441 ggcttcgatg atgatgatgc cgaaggtctc atacgtgatc gacggcacga tcacgcctag
     1501 cgcatgccgg tagtaaggcc cgatgaggtc ttgcgaggtc catcctagga atttcacgcg
     1561 tttcatcccg cgcgcttgct tgcgcaactc ccgctcgtac gtgcctgagc cggaaaccag
     1621 caagtcatag tcgccttcgc cgcgaaattc cgggagcacg ttctgtaaac ctttgatttt
     1681 ctccaggcgg ccgacaaaga gaaaataggg gcgcggatga ggcgccggcg ccgtggaaat
     1741 gccctcctca tcggccggat ccaggaaata gggcagcact tcgatcggga agggaaagcc
     1801 tcggcggtga tgcatgtccc gcgtaaagcg gctgggggag aggaattgat ccactcggct
     1861 cgctgctcgg tctcgcaagc cggtatagcg ccagaactgg ggggggcggc cggaaaccaa
     1921 cgtgcaagaa aggcagtcgg gcttctcgca ggcgcggcgg ttgtatttcc acagaacatt
     1981 catcgggcag acaagccagt gttcatgcat cgtatagagc ttgacccagt cctgatagct
     2041 cggttccatt tccaataccg ccgggccgag cagagaaatg ttatggaaat ggatgacgtg
     2101 aaagcggcgc gactcgagaa tgtcgcggat cttgcccgcc ttgagcagcg gacggccggt
     2161 ctggtgcgat agcagcgggg aaagcatccc gagcccgctc tccagcttgt gcaccgtgat
     2221 gcccggccgg gcggcgaaag tgtgcagatc gggctcgggg cggaacatgc gaaaggaatc
     2281 agcgcagtga atcacatcaa cggtgtggcc ggcgcgggcg agctcgttgc agaggcggta
     2341 gagataaatc gcgtcgccgc cgaagctgta cggcgggtag aaggttgtga ccatgcaaaa
     2401 gctaagcggc tcgggcatga cgtcgggcga cttgatcgaa gattgctagc agttcgcggg
     2461 cggccttttc ccaggatagc gaagccgcag cgcgcaggcc atgctcgcgc atgcggcggc
     2521 gtagatccgc atcggccaaa acgcggccaa gcgcatgccg gagagcgtcg ccgtcgaagg
     2581 gatcaatgca gagcgcgcct tctcccagca gttccggcag cgggctcgcc gatgtggcca
     2641 ctaccgggag gccgcaggcc gccgcttcga tcgccggcag gccaaatcct tcctgaaagg
     2701 atggcagcac cagggcggtc gcgaggttca gcagatgggc caggtcttcg tcaggaatgt
     2761 acccggtgaa aatcacgtgg cccgccaagg gcggcgcaga ggcccgcgct cggatctgct
     2821 cgtagcagga gtaaaacgaa tcgccctggt gatcgccggc cagcaccagc cgcaaatcgc
     2881 tccatctttc cagcaggacc gtgaacgcgt ccaggagccc ggccagattt ttgtgcgggc
     2941 tgaagcctcc cacaaacacc agcatgcggt cgccgggacg caggcccgcc tcgcgcagcc
     3001 gttcgctgag cacgggattt tcgatgacgc ggaaggcgcg atcgcccgct tcgccgatca
     3061 cctttacccg ctccggcgcc tccccgaaga attccaggat ctctcgccgg gaaaactccg
     3121 agacggtaag gatcaagtcc gcttggcggc gcgcgaggaa ggatttcagt ttccagttcc
     3181 agcgattcgc cggagcggga aacacgtact ccggaaattt ctcgggaatg acgtcatgta
     3241 ttactacgat cttgtaagcc cggctcacta ccggcgagta gctatatacg gtcggaaaca
     3301 gcaggcagtc gagatcggcc cgccccagcg cgcggctcat ggcccacagg tctcgcagtc
     3361 gccgccggcc gttgctccgg gccgcctcga tagcgggcgc cgacgcggag acgcaaacga
     3421 tttcgccgcc gggtggaagg ttcgccgggg gttgcgggga atcagtgaag aagacgtact
     3481 ggttgctgcg atccagttct accgccgctg ccagcagcgc ccgggcgtgc ctgccgtagc
     3541 cgcgccggtt atcccagacc gccgcatcca ctccaatccg cactgttcca atatatcgtt
     3601 tgggggctgc gcttccaagc tggccgcatg ccgaaaacaa cagcgccgcg gcggcagact
     3661 ccggtcaggc ccgaagctgg gcccgagccc ccttcccgaa gcccgatcgc accggaaggc
     3721 tgctctggcg ccgggcattg ttcttttctg caaggggggc gcctcggccg ggcgccggca
     3781 gcggctacga gcgctgaagt cccgcggatt gcaggagaaa gccggccatg ggccgctggt
     3841 cggaaatagg ccgaaaaggg cgggaaaggg gccacttgac agaccggctc gccgggaata
     3901 gagtaaggcc aggtatagtc ccctacccgg ccagcgcgcc gtaaaaaacc ggcatcgttg
     3961 aaaggaggca cacagcgatg ttcgaaatcg tcgagaaaca caagccatcc aaagaggaga
     4021 agaaagtaat ggtgctcgag tggctagctg cactggccat ttgcggcatc attggcgttt
     4081 tgttgtgggt ggtgttcgcc catggaggag gatgagctcg aggcttgtga tcggagcgca
     4141 gggaactttg cgcggacgaa ggcaagcgca tcttgacggc tggtgatacg gttgtccaac
     4201 tgtgcctctt ccacggcctc aaggatacgc cggaacagcg gtccgggctg atacccctcc
     4261 gcgatgagat cgtcgccggt gataagtttc gccgggcgaa tggcttcagg gggtagtgtt
     4321 ttccacgccc ggagaacaaa ttcgtaattc cgcagatttt tgtggctggc caggcaatcc
     4381 agccggtgga gttcgaggtg ttcctcaaat gcctccagcc gcatgaaacg cttgagggtg
     4441 ctctctcgca tacggggcgc gtctttgaat cgcaggtgat ggcgcaccag ggccacgatc
     4501 tgatcggttt gccggcgcgg aaaccgcagc cgcaaacaga tctcctccgc catggccgcg
     4561 ccaatttcca cgtggccgtc aaagcgaatc ctttccgcca cccgaaatgt cggtggtttc
     4621 cccacgtcgt gtagcaagac tcccatcgcc agggtcactg tcggccgttc catcatctcg
     4681 agcatgagca gtgtatgggt ccagacatct ccctcgggat ggaattccgg cggttgggcc
     4741 acgcccttca tggcgcttac ctcgggcaaa aggtggggaa gcaagccggc ctcctctagg
     4801 agctcgacgc cgcggcgcgc gccgccttcg gtgaggattc gcaccaactc gtcgcgcacg
     4861 cgctcaatgg ccacctgccg gatagctgca ctctggcttt ggatggcggc catcgtgccg
     4921 gcttccacac ggaatcccag ccgtgcggca aagcgcaagg cccggatcat gcgaagccgg
     4981 tcctctgcaa agcgctccga tgggtttccg atggcgcgca cgagcttggc gtccagatcg
     5041 gcgcggccgc cgacgaaatc cagaatctct ccgctcagcg gatcgagaag caggccgtta
     5101 atcgtgaagt cccgccggag cacatcctgc tcggcgctgg cggtaaacgt gacgccgctc
     5161 ggacgccggc cgtcgaggta cgggtgatcg gaccgaaagg ttgcaatttc gacttgaatg
     5221 gaatcgcgcc ggaccaacat cacgccgaat tgggctccaa cggcgatggc gtccggaaac
     5281 agttcctgga ttcgcgccgg cggtgcgtcg gtggccacat catagtcctt cggctcgcag
     5341 cccagcagca tatcgcgcac gcagcctccg gccaggtaag cagcgtggcc gcttgcccgg
     5401 agccgctcca cgatgctgtg cgccagctct cgggcggtca tgtgcgccgg tctttcgccg
     5461 gccccggacg atttttgccg ggcaggccgg cggggttctc gtggacccgc tcccgccagc
     5521 ggcgccggag accgccggcc agctcgatgc tgcgctcgcc gtagcggtca cgaagagaat
     5581 cagcggcttg gagcgcccgc ctccatttct cccgccgctc gctctctagc agggacatct
     5641 ggcccggctg cccgctgagg cccgacactt gcacgcccaa tagacggatt ggcgggggcg
     5701 cggtgcggcg ggaatccgat cccggcaggt tttggcccca gttctcccgc aaaagttcct
     5761 gtgcggcttg aaagatcacc gtgtcgagtt gcgtggcctc gtcgagggtg cgagcgcggg
     5821 tgatcgtcga aaagtcgctg tgtcgcagct tcagcgtgat cgtgcgccca taaaggccat
     5881 actcccgcag acgccggccg gccatttgcg agagcctggc gagcgtggcc tccagcagtt
     5941 ctggatcttc cgtatctcgt ccgaatgtag tttcgtggct gaccgatttg ggagcttcgt
     6001 gagcgccgac cgggcgtgcg aaccaggctc cggcgtcctc ccctcgcgct ttgccggcga
     6061 gcgccgtccc tgtgattccc cattcgtgat cgagaacgcc gatttgcgcc gccgccaggt
     6121 ctccgatcgt gcgaattccc tcgaagcgca gacgccgctc catgacccgc cccactcccg
     6181 gaatgcggca gacgtcgagc ggcgcaagga aatcgcgttc cgcccccggc acgacccaca
     6241 acactccaca cggcttggcc aggtcggagg cgatttttgc gatcaggcgg ctggtcccga
     6301 tgccaatgga acagggcagg tgcgtttctc gctcgaccgc ctcgtgcagc agatgggcgg
     6361 cccgcagggg cggcccgaag agccgctcgc agccggtcaa atccatgtat gcctcatcga
     6421 tggacgccat ttccacctgg ggcgagaagc ggccgaagat ttcatagact tgcttggagt
     6481 gttcgcgata gcggtccggg tgcccctcaa taaagagcgc ctgcgggcac aatcgatacg
     6541 ccgtccgcag cggcatcgcc gaatgaacac cgaatttgcg ggcctcatac gaggccgcgg
     6601 cgactacgcc ccgttcgtgc ggccgcccgc ccaccactac gggcctcccc ttgagccggc
     6661 ggtcatagag ctgctcgacg gagacgaaga aggcgtccat gtcgatgtga aaaatggtcc
     6721 gcatgggagc tgccgcccgc aacccgactc taagaatagc tcgtttcgtt ccgcaaaatc
     6781 ggccgcctgg ccggaacaga cccggcggct ctcagggtgg atcgcttcag ccgagcgctc
     6841 gattgcggcc gggctttgcc ggccccgcac taaactatac tgggcaaatg cggcgactct
     6901 ttctcggggc gatcctggca ggcgcgatcg cgattgcggc cggagcggga tggtggcttt
     6961 ggcggcaaaa acaaaatgaa agatttttgt tccgccgtct gcccggcgaa aattcgctcc
     7021 tgctttacct ggacatgaac aaactgcggc gttcggaggc gctgctgccg ctggttcgct
     7081 cccaggtgga tccggatccc gactacgccg cgttcgtcag gcagacaggc ttcgattatc
     7141 agcgcgatct cgatgaagcc gccgtgtgct atctcgccga ccgcgtttat gtgctcgccc
     7201 gcgggcggtt cgacgagccc cggctcagcg agtacgcggc ggctcagggg gggagctgcg
     7261 ccggcggcgg ggtctccgga aagacgcgag tctgctggat gcctgccagc cggccggacc
     7321 ggaaaatttc cttttttatc ctctcaccgg gagtgttagc gctggccacc gcccccgagc
     7381 ccgatgctgt gttgcaactc gaggtcctgc ccgtccccag cgcagagcca ttagcccagg
     7441 cggcggccgc ccggtccaaa ggaatggcct tgctctgggc cacggcggcg ccgccggccc
     7501 tggatcggct cctcagcgaa accgggggcg gcgtctctcc caacctgctg ttgctttcga
     7561 gggcgctggc cgcggctcag cgagcctatc tcctgcttat ggaccgctcg ccaaacctgg
     7621 agctgtcgct ccaggccgtc tgcgcctccg aagcgcaagc tggggagctg cgccgcctgc
     7681 tgcaaggcct gaacgatctg atcggcggac tgctgcgggg cccggcctcg aaaggccctc
     7741 ccagcgactg gggaagactg ctcgcctcag cggtcatcgc gcagaaccag aacacggtga
     7801 ccgccacctg gacgctggac cagtccatct tgcgcaactt cggaccggcg caaggcgcac
     7861 ggtagcgccc tgtggagatt cgaccggagt cgatcctacg cagcgatcgg tcgattgccg
     7921 cagctccggc tttctttata gaaccggtcc caggcggggc tggaggtgag cgaaatcccg
     7981 gaagggagcc ttcaaatacg gaatcgggtt gagcggcgtg tgattccggc gcacttcgta
     8041 gtggaggtgc gccgatgtgg tccggcccga gcgcccgact cgcccgatca cttccccgcg
     8101 gcgaacgacc tggtaggggc gcacgaagca ctccgataag tgagcgtaat aggtattgat
     8161 gttggtcttg ccgtgctgca agatgatcag ccggccgtag ccgttggcga atccagcgga
     8221 agccacaacg ccgtctgctg cggccacgac gggcgagccg tagggactgc taatatccac
     8281 cccggcgtga aacgcgcctt ccccctggaa tggatcgata cgggacccga acgccccggt
     8341 cagggcgcct tgcacgggcc agatcgaggg cacggtgttc tcgagccaat gccacggcat
     8401 gctttccccg attccattgg aagtcagcgg aacgttctcc agaaagttga attgctcgag
     8461 agacgcctgg tagctgggaa ccagcgccgc ttcctgcgct atgtcgtcgg gaccgagcag
     8521 cttccgcttc agaccatagg cgactgaaat ctcgctcgcc agggtctcca gggaggccag
     8581 ttgcgtattg gtttgcgcga ctacctgctg gaggttgtcg tagcgcctca tcaaggcttc
     8641 cttctcgctg cggaggttgt tatagttaga cactttaaac agcatccgcg cgtagctcga
     8701 aacaaaaccg aaacacgtaa tgcttccgag caccgcgaag gccatgatta cgtacaggaa
     8761 gtactgcggc acctgaatac ggtgcagccg gccatgaacg gaatgagcca atacgacaat
     8821 gaaatagttt ttcctcatcc aaacctcgct cagaaggtgg tcacccggct tttgcgggaa
     8881 gcaagggccc gacttccaag acggagtgaa ctgtaataat taagacttct taacaaaaag
     8941 ttggactgta acaagccgta catggttctg tcaagtccaa aaaggtctta aatcaataca
     9001 atccattgaa aaaaaacgag ttaagtatta tccagagcat tcggcgcagg gccgccaaag
     9061 cggcacccca agccgcccgc atccgtctcg gcatcggaga tgacgcagcg gtgctggagg
     9121 ctctcccgcg aggggaagaa ctactggtga ccaccgattt gctgatcgaa ggttcccact
     9181 tccgtcgcga gcgccatccc gcccgcgctc tgggccacaa ggccctcgcc cggggtttga
     9241 gcgacatcgc cgccatgggc gggcagccgc tgtacgctct gctttcggtc tgcctgcccc
     9301 cctgggccga cgaaaaatgg agaaaacagt tttttgcagg attgtttcat ctcgcccgga
     9361 gcgctggcgt cgccttggtg ggtggggatt tgtccgcggg agggcgcttt gccgccgaca
     9421 ttgtagtttt gggatcggcg gctcgcggcc aggttctgca acggtccagg gcggcgccgg
     9481 gcgaattgat ttacgtctcc ggcagcctgg gaggctcggc tttagggctt gcacggctcc
     9541 ggcgggggca gtgcagccgg gctgatccgg ccgtgcggcg ccatttgtat cccatccctc
     9601 gcctggctct gggacgattc ctggcggaaa aactgcacgt tcaagcggct atggatctga
     9661 gcgacggtct ctcgctcgat ctgttccgga tggcggccga aagccgggta ggcgcagaga
     9721 tctgggccga ggctcttccg gtatttcccg gcgcgaatcg ggggcaagca ctgcatgggg
     9781 gagaagatta cgagctcctg ttcacattag gttccggccg gcaggtcccg cgaagcttcg
     9841 agggcgtccc gctgaccgcg atcggggtgg tgacgcggag ccggcggctc agcctgatcg
     9901 gcccgggcgg ccgccgcacc cggctgccgg tcggcggctt ccaacacaag ctggcttgaa
     9961 cccgtccccc gttgcctgaa ctgtctttgg gaatgtacag gcgccgattc ggccggcttc
    10021 cgtgggaaaa agcggcatgc gattgccctt taccagcgag gcgcgcgtga ggcggcttgc
    10081 ctcggatttt tcggagctat aatgccccta tgttcaacgc cgacctgaat cgcagagatc
    10141 tgtttcgcgc tgctgccacg ggcgcggtag ccctgacggc ggcttcctac tcccgcatcc
    10201 tgggcgccaa cgaccgcatc aacctggggg tcatcggctg cggcgcccgc gggctctccg
    10261 acatgcggga cttccagaaa aatccctccg tggcggtcac cgccgtctgt gacatttacg
    10321 gcgcacagac cgatcaagct ttgcagcgcg ctcccggcgc gcagggcttc agcgaccacc
    10381 gcaagctgct cgagctgaag aatgtcgatg ccgttttgat cgccacaccc gatcactggc
    10441 acgccggcac gacgatcgac gccttgaacg ccggcaagga tgtctacgtg gaaaagccgc
    10501 tcaccctaac gatcgacgaa ggacctaaaa tcgtcaaggc cgcgcgtgtg aacaaccgca
    10561 tttgccaggt gggcatgcag cagcgctcgg gcaggcatta ccttcgcgcc aagcaggagt
    10621 actttgctac gaacaagctc ggcaagatca ccctggcgcg cacctggtgg cacgggaaca
    10681 cgtatcacct gcgcaaggcg ccggaatcgc tccagcacaa accggacaat ctggactggg
    10741 ccagattttt gggcccggtc aaatggcggg actgggatcc gcagcaatat tacaacttcc
    10801 gcgcctatct ggatttcggg ggcgggcagg tgacggacct gttcacccac tggatcgatg
    10861 tggtccacat gtttatggac caggagattc ccatctcggc gaccgccgcc ggcggcatct
    10921 attactacaa agacggccgc accgcgcccg atacgatcaa tgtgctgctg gagtatccgg
    10981 gcgagtggac cgcgacgttc gaa
//
LOCUS       DASREK010000150         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_76351, whole genome shotgun sequence.
ACCESSION   DASREK010000150 DASREK010000000
VERSION     DASREK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76351"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..764)
                     /locus_tag="VFA54_07485"
     CDS             complement(375..764)
                     /locus_tag="VFA54_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS50685.1"
                     /translation="MNDLPVLLVIEDEHSIQDLIEDALTEGGFAFDILSSGEEALTAF
                     GGHLKNYKALITDIGLKGRLSGWEVASQIRERDPGFPVIYMSGAHADEWASKGVPNSV
                     MLTKPFAAAQLVTAVSNLLNAAPPAAE"
     gene            1120..1347
                     /locus_tag="VFA54_07490"
     CDS             1120..1347
                     /locus_tag="VFA54_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50686.1"
                     /translation="MIFRNIQRRRARRVFGGLLSEEGLDKVMSELSAWDCFIQSLPRW
                     ALPIFRRRPTNEELAASLAMFDKAAQSERGD"
     gene            1458..1643
                     /locus_tag="VFA54_07495"
     CDS             1458..1643
                     /locus_tag="VFA54_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50687.1"
                     /translation="MTDAPFKVGDRVKKRSGYEYPGLIVSVFTNRAGAVRYVVEADHP
                     AFAGMLHIFNGDQLEHR"
     gene            complement(1764..1943)
                     /locus_tag="VFA54_07500"
     CDS             complement(1764..1943)
                     /locus_tag="VFA54_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50688.1"
                     /translation="MVNAIKVLRTTADACEQHAKQVTAGAIKAELFDIAAEWHWLAGE
                     AARLNDRVKELETAA"
BASE COUNT          459 a          622 c          687 g          420 t
ORIGIN      
        1 cgcttgcggg gtcctaatcc gcatggttca gcattggcga tcataatata cagccggcgg
       61 ggagtctgtt ccctagtgca cacaagcatg tggcgtggaa atccggttga tgggcggcgc
      121 ctcgaaatgg aggcgagatg gcgctgcggc acagtcggga cgcgcgcgcc catggaggcc
      181 cgcttgggac aatgaagctg tcggatgaga gcggttagga acttccgccc gattgccggt
      241 tttcccgcac ggcgaggttc cccccgaaga tcgccaggag acggcaacgc cccagctctt
      301 cgttccagcg agctggggcg ttgttttgcg gcgctccgca gggatatgga acaggcccgc
      361 caaccgaagc ggcgttactc cgcggccgga ggcgccgcgt tcagcaggtt ggaaacagcg
      421 gttaccagct gcgctgctgc gaacggcttt gtgagcatga cgctgttggg gacgcccttt
      481 gatgcccact catcggcgtg cgcgccgctc atgtagatga cggggaaacc gggatcgcgc
      541 tccctgatct gcgaggcgac ctcccagcca ctcaatcggc ccttcaagcc aatgtcggta
      601 atgagcgcct tgtagttttt gaggtgcccg ccaaacgcgg tcagggcttc ctcgccggag
      661 gaaagaatgt cgaaagcaaa gccaccttcg gtcagcgcgt cctcgataag gtcttgaatg
      721 gaatgctcgt cctcgatgac gagcagcacc ggcagatcgt tcacgttacc cacccatccg
      781 catcgttgcg ggcaccatgc cggcacgaac gataagtgca gaagtggact ttggttcctt
      841 gggcgaatgc ctacgccgcg gtctccagct ctttggccct gtcgcgcagc cgggcggctt
      901 gtccggccag ccagtgccac tcggcggcaa tgtcgaataa ttcggccctg gctgcactcg
      961 cagcgacctg cttggcccgc cgctcggggt cgtcggcggt agtgcggagt agtttgacgg
     1021 cgatgaaaca cgttgagatg aagatccgag cgagatggac tcattcaagg tatgtttgaa
     1081 tgaaagcggc cgcccagccg gggcggcctc tggagcgcga tgatatttcg caatattcaa
     1141 agacgacgcg cgcgccgcgt cttcggcggg cttctttccg aggaagggct ggacaaggtg
     1201 atgtccgagc tttccgcgtg ggactgcttc atccagagcc ttccgcgatg ggcattgccg
     1261 atctttcggc gccgccccac caacgaagag ttggccgcgt cgcttgccat gttcgataag
     1321 gccgcccaaa gcgagcgcgg agattgatcg gatcaggaca ctcccgaatt cagcctggaa
     1381 taccggagtc aaatacggac ggcatccgcc atcgaaatgc gagagaccgc caatcggggc
     1441 ggcctagagg ggataaaatg acagacgcac ctttcaaggt aggcgaccga gtcaagaagc
     1501 gctcaggtta cgaatatccg ggcctcatcg tgtcggtctt tacaaaccga gccggggccg
     1561 ttcggtatgt cgtggaggca gaccatcctg ccttcgccgg catgttgcac atcttcaatg
     1621 gcgatcagct tgaacatcgc taagccaaac gaggccactg ccaaaatcaa actgggccgc
     1681 cagggggtaa ataggccagc gcccacaatt ctggtgctgt cccaattagg gcaccccgcg
     1741 acggcaggtc aggacactgc cgcttacgcg gcggtctcca gctctttgac cctgtcattt
     1801 agacgggcgg cttctccggc cagccaatgc cattcggcgg cgatgtcaaa tagttcggct
     1861 ttgatcgcac cggcagtcac ctgcttggcg tgttgctcgc aggcgtcggc ggtggtgcga
     1921 agtactttta tcgcgttgac catggtgcaa tccccaaaat gaaatctgca cacagttagc
     1981 gtgtgcagta ggcagcaaac aactccgggg aattgcggga gcggataacg aggtaaaggg
     2041 ttcgtaaacg caggacaccc gcagagggaa agatcgggct agagggactt caatcaaaat
     2101 atgtctgaaa tgagagaggc cgccaactga ggcggcctca atgccaatag ttagtggaga
     2161 acagataggc gaaggcgatt gctagcag
//
LOCUS       DASREK010000151        12181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1398632, whole genome shotgun sequence.
ACCESSION   DASREK010000151 DASREK010000000
VERSION     DASREK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12181
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398632"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFA54_07505"
     CDS             <1..405
                     /locus_tag="VFA54_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZS50689.1"
                     /translation="RSLPMTSGRNADGVRIKLCHATPSDPLFAYCGPDSPQWEREVEL
                     AGADMLLVGHTHLPFVRRFGKQQVVNPGSLGQPKTGRPAACYAVWEKGEVELRSFNYA
                     FEETIRKIHALPVDQKVRARLADVLRNGGLPP"
     gene            597..1316
                     /locus_tag="VFA54_07510"
     CDS             597..1316
                     /locus_tag="VFA54_07510"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZS50690.1"
                     /translation="MRSVPNFEEPLCVRVPAGWFLMGCDQGRDEEQPVHRVWVDEFEM
                     AALQVRRRDYAIFLQATQNPEPRYWRSPEFSDPEHPVVGVNWMEASAYCAWLSDLTGR
                     HYRLPTEAEWERAARGGREGELYAWGNTPPEAHAAYQRRWGGEVTGPLPVGQDQPNPF
                     GLFDMGENVHEWCLDWFDKEYYAVSPERNPRGPRAGVRRASRGGSWRHHIKVSRCGAR
                     SSIPPAFQYADYGFRVVRDVL"
     gene            complement(1350..2330)
                     /locus_tag="VFA54_07515"
     CDS             complement(1350..2330)
                     /locus_tag="VFA54_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50691.1"
                     /translation="MESIFRNFACALLITCQAPGIRAQETPGQPPSTQQPSPSGQPAP
                     QQQTIRQEPAAGQPASEGPRNISDGSFSLDVFYWLTGARPQLRGSITTTTSTTVGLTT
                     KTTTTTTPSNLAFPGKSEPARGATLSIPAGRYNALRFSYFQTRGGGNTTATQNTNLFG
                     AVYNAGDFLTAHYTLQNAKVSWDYLSYRLPPRATGFRVKTLWEIQYTWLRSSISAPEK
                     TPSVDSSGNLITNSSTGTRSLIFPTLGAGLEHAVSKNFRWEAKASGFGIPHHGNIWDT
                     EAWAAYRFGQLEARAGIKAFHFKTSPKNDIYVGGATLSGAFFDVRWYPKL"
     gene            complement(2690..3118)
                     /locus_tag="VFA54_07520"
     CDS             complement(2690..3118)
                     /locus_tag="VFA54_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50692.1"
                     /translation="MLIQAGDAWALAANILKQKFTKRKITTSAASYRRGVPVDIRPGD
                     EGPLAFERSRVVIHLEGRLPSEAITATMRQQNRRFVCHLLPPVFRGPQFVRSQGRNWH
                     TPWTGRVRGACAGRSQHPAGRAAASRGPAPPVNVCPCLNL"
     gene            3530..3715
                     /locus_tag="VFA54_07525"
     CDS             3530..3715
                     /locus_tag="VFA54_07525"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP-like transmembrane domain"
                     /protein_id="HZS50693.1"
                     /translation="MRENIGDTEQKVRMAIGSAAAAAAIFAPLRYKWKGVLSAVAVMG
                     LLTGAMGYSPLKRVLGI"
     gene            4027..5388
                     /locus_tag="VFA54_07530"
     CDS             4027..5388
                     /locus_tag="VFA54_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZS50694.1"
                     /translation="MLFATVYSVFFISNFLLFLRLRRGLMAWFPESSPGRLQKLLAVF
                     FLLVNGPLLIAAGMSWAGWSLEHIPEPLVHWFFYPSLAWLAMLVGYFLVAVPKGLLFG
                     VGRGARWLSSYAASPFAKPRHEAAGSQPMLEESRAGQPEGGPASFFRSSPASRRRFLM
                     RLAPAAPAALLGTAAYGVYGTKGDLDISPEVMIPIAGLPRELDGFRITQLSDLHFGPY
                     IRSRDMEPVVETANALRSELIVITGDILDRSLHLLPEAAESLSRLQAPYGVYAILGNH
                     DYYSDQRNPLTGYPGCNRLMEGLAPAGIRFLRNERASIRVRGAELLLAGLDWLGATRG
                     DPNVYQQQWTRNALGKILGGSNPEAVRVLLAHHPHTFMEAEQFGIPLTLAGHTHGGGQ
                     VVLGQINGVPIGLGALRFRYISGLYKEGSRHLYVNRGIGYFGIPIRINCPPEISRFRL
                     VRA"
     gene            5551..6759
                     /locus_tag="VFA54_07535"
     CDS             5551..6759
                     /locus_tag="VFA54_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZS50695.1"
                     /translation="MSFPSGRHFLQIPGPTNVPDRVLRAMARPTIDHRGPEFARLGTE
                     VIEGLKEVFKTTGRVVIYPASGTGAWEAALVNTLSPGDKVLMFETGHFATLWWEMAGK
                     LGLNAEFVPGDWRHGVDPAVVEAKLAEDRRHEIKAVAVVHNETSTGVTTRIAEVRKAM
                     DRAGHPALLMVDTISSLASMEYRHDEWGVDVTVAGSQKGLMLPPGLSFNAISEKALAA
                     SKSARLPKSYWDWQAMAGPNKSGFFPYTPGTNLLYGLREALTMLREEGLENVFARHAR
                     HGEATRRAVRAWGLEVLCLNPDEYSSSLTAVLMPAGYNADELRKVILENFNMSLGNGL
                     SKLQGKVFRIGHLGDFNDLMLAGTLSGVEMGLALAGVPHSKGGISAALEYLAQAASET
                     RRQPVEATVA"
     gene            6785..7114
                     /locus_tag="VFA54_07540"
     CDS             6785..7114
                     /locus_tag="VFA54_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HZS50696.1"
                     /translation="MKPTELQVLYLTTTGRKTGLPRQIEIWFVAADGKLYLLAEHFRK
                     ANWVRNIEQDPRVRVRLGEKEFAATARVLDEEHDRDSWRLAQRLGREKYGWGEGLPVE
                     ITPSEPL"
     gene            complement(7385..9427)
                     /locus_tag="VFA54_07545"
     CDS             complement(7385..9427)
                     /locus_tag="VFA54_07545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS50697.1"
                     /translation="MPKIASRILWPAWVLFTIAVTARPFLWHLEYYRHPGKEFFAAYL
                     ALLALLIVASFLYIRLRQSRPALTAWEPRIFFAFFLAFFLFYAFRPTLYSLLTLFVCF
                     GLGRLTLGRLAESALEQLTLFPAAGLGIMSTLLFALGALHLLYRSAVLALLIMGALLL
                     RANAKTLWDLLRRAAEKYPSAAADPLWGLCLIFLFVSMIASAMVVLSPEIAFDPVSFH
                     FVLARDYALHHRLEIVPYFPYSYFPQGIEILYTLGFLLEGQITAKALTYAFFPLSAAS
                     VAMIGQRWFAESRAGALAAMAFFATTPFIDWTGSVAKNDLALALYLLLALYGAVRWTE
                     TRRFGWLAAAALFLGFGFGVKHVAILGAVPWALIVLWNWRSFRLRPRQAAALAAVFFS
                     AGLYWHVRTLILTGNPLYPEATRSAVRSTTAAGHPQLTRLDRLGIYLSVPWRIHFRGL
                     PAFESPSPNPCGFVVIAFLPLLFWPRRNSSPAARLVLVFCAIYFLYWAAILIKVRYAI
                     AAFGALFACLGDRVCRFHSDRNDAFRRYCVLALAAYCLAFSLTLVLILQINVPRLELF
                     ARRINPDQFLRKTLVTYPSIAALNRLAQPGERAYSVGNCSSFYADVGFRCYYDAGGNY
                     SLDQIRGELETMRYQYLLVSNAWAEPHHMHVIDRLFHPLLVYQDDSFRIYRLRRVR"
     gene            complement(9415..10398)
                     /locus_tag="VFA54_07550"
     CDS             complement(9415..10398)
                     /locus_tag="VFA54_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS50698.1"
                     /translation="MGVIPFACPACKGPVDFAASAYRCGRCRKEYPVLCGIPDFRILP
                     DRYISIEDDRRKGQRLFAETSRRSFHEMLDFYYSITPEVPPPLARKFTAHALAEPAIG
                     EQVLAEIGESSPDGALLDVGCSTGGLLIAAAGRRLAALVGVDVAFRWLVIGTARLREA
                     GVQAQLVCANAELLPFPCGRFTVITATDLIEHVAEPARVFAECYRVAASGGAVYISTN
                     NRFALAPEPHAGIWGVGWLPRAMQAGYVRFVSGRAYRNIVLRSARELDQWVRKAGFED
                     CAAAPAPLAGRVSNTRWFAPVESLYNRLRRAPGSSGLLRWIGPRLQLLCRK"
     gene            complement(10389..11147)
                     /locus_tag="VFA54_07555"
     CDS             complement(10389..11147)
                     /locus_tag="VFA54_07555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZS50699.1"
                     /translation="MLVFPCGSLTVTSVDSWRAILTYHSLDDSGSVISVRPSVFARHM
                     EELAGQGVNVVRLTELPAAPAPALALTFDDGFQNFFESAAPVLARYNFPATVFLVTNY
                     CGGANDWPSQPPDIPRLPLMSWPQIVELSRAGIEFGAHTATHTNLAQLEETAMREEIL
                     SSKQRIEEATGRPVSAFAYPYGSMPEPAQRIVARHFAAGCSTRLGWVTAESRPEALER
                     LDIYYLRYPFLIRRLFEPSVRGYLALRGALREWA"
     gene            complement(11211..>12181)
                     /locus_tag="VFA54_07560"
     CDS             complement(11211..>12181)
                     /locus_tag="VFA54_07560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS50700.1"
                     /translation="SAEAMETGRRASGSVLRALMTPAAPEISIIVPVRFARQTINSTL
                     KALLEQCEGIAAEIIAVVSSTDPTREALRELACDPKLRIIEMPGRRSVPELRGEGVRL
                     SRGKLIAITEDHCSFPPGWAAGMIDAHRTREIAAVGGPVENGRAGGCLDWAIYFSRYL
                     GSMPPLDSGPAASLPGNNACYKREALERCAPLLAGGFWENDVNRELLALGYILWLDPR
                     LTVIHRKPYRFAPYLALRYRHARCFGGMIARTLTGFGRMRRILFSPLIPALLMLRSMR
                     LIQAKKRRRREFLVASPALLLCYLVWFWGEVAGYIAGAGAACSETD"
BASE COUNT         2506 a         3583 c         3842 g         2250 t
ORIGIN      
        1 cgaagcctgc ccatgacttc cgggcgcaac gccgacggag tccgcatcaa gctgtgccat
       61 gcgacgcctt cggacccgct gtttgcctac tgcggccccg attcgcctca atgggagcgg
      121 gaggtggaat tggccggagc cgatatgctg ttggtcggcc acacgcacct gcctttcgtc
      181 cggcgatttg gaaaacaaca agtcgtaaat ccgggaagct tgggtcagcc gaagactggc
      241 cggcctgccg cgtgttatgc ggtgtgggaa aaaggagaag tggagcttcg gtcgttcaat
      301 tacgcgttcg aagaaaccat tcggaagatt catgcgcttc ctgttgacca gaaggtccgc
      361 gccaggttag ccgacgtgct gcgcaatggc ggccttccgc cgtgaacctt gagggactgc
      421 ttgcgccgcc ttcccggttc tgcggccaga tcgggcgatc gctgcggctg cggcgagcag
      481 ccgagggcta gtggcggtat gcaggtatgg aaatcggcgt tgccagatgt tcctgcgcct
      541 gcggaggccg catgaggtct tatgtttgac tcgaacgggg aaccgttggt cgaaagatgc
      601 gttctgttcc taattttgaa gagccccttt gcgtccgcgt accggcgggt tggtttctca
      661 tgggatgcga tcagggccga gacgaagagc agcccgtaca ccgggtgtgg gtggatgagt
      721 tcgaaatggc tgcgttgcag gttcgccggc gcgattatgc gatctttctc caagcaaccc
      781 agaacccgga gcctcgctac tggcgctcgc ccgagttcag cgatcccgag catccagtag
      841 taggcgtgaa ctggatggaa gcgtcggcct actgcgcctg gctctctgat ttaaccggcc
      901 ggcattatcg gctgccgaca gaagccgaat gggagcgagc cgcgcgcggc gggcgagaag
      961 gggagctcta cgcctgggga aatacgccgc cggaggcgca cgccgcttat cagcgccgtt
     1021 ggggcggaga agtcaccgga ccgctgccgg tcgggcagga ccagcccaat cccttcggcc
     1081 tgtttgacat gggggagaac gttcacgagt ggtgcctgga ctggttcgac aaggagtatt
     1141 atgcggtttc gcccgagcgc aacccgcgag ggccgcgagc gggcgtgcgg cgggcctcgc
     1201 gcggcggatc gtggcggcat catatcaagg tgagccgctg cggcgcgcgt tccagcatcc
     1261 caccggcgtt tcaatatgct gactacggct tccgcgtcgt gcgcgatgtc ctctagcttc
     1321 tcccatgcaa gccggttttc taagtcgctt tacaattttg gataccagcg aacgtcaaag
     1381 aatgcgcccg acaaggtggc gccgccgaca tagatgtcgt ttttcggaga ggttttgaag
     1441 tggaatgcct tgatgccggc gcgggcttcc aattggccaa aacgatacgc cgcccaggct
     1501 tccgtatccc atatgttccc gtgatgcggg atgccgaaac ccgaggcctt ggcctcccac
     1561 cggaaatttt tggaaacggc gtgctccagg cccgccccca acgttggaaa aatcagactc
     1621 cttgtgcccg tcgagctgtt tgtaatgaga ttccccgacg aatcgacgga cggagttttc
     1681 tccggcgccg aaatcgagga tcgaagccac gtatactgaa tctcccacaa ggttttcaca
     1741 cgaaatcccg tggcacgcgg cggaagccgg taggaaaggt aatcccagga aaccttggcg
     1801 ttctgcaagg tgtagtgggc cgtcagaaaa tcgccggcgt tgtatacggc gccgaacagg
     1861 ttcgtgtttt gcgtggctgt ggtattgccg ccgcccctgg tctgaaagta cgaaaatcga
     1921 agtgcgttgt atcgtcccgc cgggatgctc aaagttgcgc cccgggccgg ttcgcttttt
     1981 ccgggaaagg cgaggttgga cggcgtggtg gtcgtcgtgg tcttcgtggt gaggccgacc
     2041 gttgtggagg tcgtcgtggt aatgctgccg cgcagttgcg ggcgcgcccc ggtaagccaa
     2101 tagaagacat ccagggagaa cgatccatcg ctgatattgc gtggaccttc cgaagccggc
     2161 tgtccggcgg cgggttcctg gcgaatggtt tgctgttgcg gcgcgggctg accggaaggc
     2221 gagggctgtt gcgtagaggg cggctgcccc ggcgtctcct gcgcgcggat tccgggcgcc
     2281 tggcacgtga ttagaagtgc gcacgcgaag ttacggaata tcgactccat aagacctcga
     2341 agactcagta ggaatgcgaa actcaccggc ttggaatttt tcataaggag cgccccgctg
     2401 tctcaggaga aaagcgggaa acgccggttc ccatttttca acgaaagcat ctgcatactc
     2461 atggggcttg aggggcttgt gagattggtc tgtgtttgcg gaccgcggtc tttcttttct
     2521 tgttcttggc ccgccgggca gcgcgtctgg cgcatgcgtc ggcttctcct ctgtgcaaag
     2581 cacacatgaa ctgtttccac attatttgag ccgcagactt acccctgaac aatattcgca
     2641 caggctcgcc agagagttcc ccaaaaatta tcatcccaac agcggaactt tacaagttga
     2701 gacacgggca gacattcacc ggcggggccg ggccgcggct ggctgccgcg cgtcctgcgg
     2761 gatgctgaga tcggccagcg caggcgccgc ggacgcgccc cgtccaaggc gtatgccaat
     2821 tccggccttg tgagcggacg aattgcgggc cacggaacac gggtggaagg aggtgacaaa
     2881 cgaagcgccg gttctgctgc ctcatcgtcg cggtaatcgc ttcggaagga agccggcctt
     2941 ccaggtgaat tacgaccctc gagcgctcga aagccagcgg accttcgtcg ccgggacgaa
     3001 tgtctacggg cacgccgcgc cggtacgaag ccgcagaggt ggtgattttg cgcttcgtaa
     3061 atttttgctt gagaatgttc gccgccagcg cccacgcgtc cccggcctga atcagcatca
     3121 ccctcgggag ccagacggcg gcataatccc tgtcattggt tatttgtggg tctaaatact
     3181 ttaggtctaa cgtttgggct tcatcgagcg ttcctctgag gcaggcataa gggatttcag
     3241 cggcgagccg cccgcctcac cgcctgagga gcagagcctc caggcaaaac ggaaagggag
     3301 gtgcggcgga cagcggttcc ttccttgttg cattgaccgg aaatgcgctg cggctttcag
     3361 gcggttccga acgccgtttt gcgcccaaac agacgtttca ctcggcttgt gtgcgttggt
     3421 tcacttactc ccccggcaac tcacggtatt ctggcacgta cccagggcgg agcagcacgg
     3481 gcaaaaaggc cgcacctgga gaaatctatt cacgagcgag gagacattca tgagggaaaa
     3541 tatcggcgat actgaacaaa aggtgcgtat ggctattgga tcggctgccg ctgctgcggc
     3601 gatattcgct ccgctccggt acaaatggaa gggcgtgctg agcgctgtag ctgtcatggg
     3661 cctgctcacc ggcgccatgg gatactcccc cctgaagcgc gttttgggaa tttagaaacg
     3721 ctgcccctga tcaatcgatc gctgcatcgg cgaaggcatg tatagaaatg cgggcgacgc
     3781 ttccctagcc tggtggcggc gcccccgcat ttctgcgttc atttgctact tgccctccgg
     3841 cgggcgcccc agagaatgcg ctttccaagg cagcgagtga tcaatcgccg ctgcgaggcc
     3901 ctgccgctaa cgtcttcatt tgcgggctcc aggttggtga cgcggcgatt gccagctgct
     3961 gttccacgat cccgttccca caccggctga ttccggcggc ggtaaaatga agattcgatt
     4021 cggcccatgc tttttgcgac cgtctacagc gttttcttca tttccaactt cctgcttttc
     4081 ctgagacttc gccgggggtt gatggcttgg tttccggaaa gctcgccggg ccgccttcaa
     4141 aagttgctgg ccgtcttctt tctgctcgta aacgggccgc ttttgatcgc tgctgggatg
     4201 agttgggcgg ggtggagcct ggagcacatt cccgagccgc tggtgcattg gtttttctac
     4261 ccaagtctcg cttggctggc gatgctggta ggctacttcc tggtagccgt tccgaagggc
     4321 cttctttttg gcgttggccg gggcgcgcgc tggctttcca gttacgccgc gtccccgttt
     4381 gcaaagccga ggcacgaggc tgccggctcg cagcccatgc tggaggaaag ccgcgccggc
     4441 cagcccgaag gcggtccagc ctcttttttt cgcagctctc ccgcctcccg ccggcggttt
     4501 ctcatgcggc tcgcgccggc cgctcctgcg gcgctgctgg gaacggccgc gtacggagtt
     4561 tacggaacga agggcgatct ggacatctcg cccgaggtca tgattccgat cgctggcttg
     4621 ccccgcgagt tggacggttt ccggatcacg cagctctccg atcttcattt cggcccgtat
     4681 atccgctctc gggacatgga gccagtggtc gaaaccgcca atgcgctgcg atcggaattg
     4741 attgtgatta caggtgacat tctagaccgc tccctccatc tgctcccgga agcagccgag
     4801 agtctctccc ggctgcaagc gccttatggc gtctacgcca tcttgggcaa tcacgattat
     4861 tattcggatc agcgcaatcc tctcaccggc tatccggggt gcaaccgcct gatggagggt
     4921 ctggcgcccg ccggaatccg cttcttgcgc aatgaaaggg cttccatacg ggtgcgcggc
     4981 gccgaattgc tgctggcggg tttggactgg ctgggagcga cccgcggcga tcccaacgtt
     5041 taccagcagc aatggacaag gaacgccctg gggaagattc tggggggcag caatccggaa
     5101 gcggtccggg tgctgctggc ccaccatccg cacaccttta tggaggccga acaattcgga
     5161 attcccctca ccctggcagg ccatacgcac ggaggcggcc aagtcgtgct gggacaaatt
     5221 aacggggttc caatcgggct gggcgctttg cgctttcggt atatctcagg cctttacaaa
     5281 gaaggctccc ggcatctgta cgtgaatcga ggcatcggct acttcgggat tcccattcgc
     5341 atcaattgcc cgcccgagat cagcagattc cgcctggtgc gcgcgtgagt tgcgcggaaa
     5401 ataggaaaac cgccgcccgc gaggcctcgc ggcgagtccg gccttattgg tcccgctagg
     5461 aaaccagcct gccgcagttg cgatcggcag gccaggaatg cgacattggt tattgttagt
     5521 ccgttttctt ttaaccttga ggattgctct atgtcatttc ctagtggacg ccattttctt
     5581 caaatcccag gacccaccaa cgtgcccgac cgcgtcctgc gcgcgatggc ccggccaacc
     5641 atcgatcatc gggggccgga gttcgcccgt cttgggacgg aggtgatcga aggcctcaag
     5701 gaagtgttca aaaccaccgg gcgagttgtg atttaccccg cttcgggaac cggggcttgg
     5761 gaggcggctc tcgtcaatac tctctcgccc ggcgacaagg tgctgatgtt cgagacgggg
     5821 catttcgcca ctctctggtg ggaaatggcc gggaaattgg gcctaaacgc cgagttcgta
     5881 cccggagact ggcggcatgg cgtcgatccc gcggttgtcg aagcgaagct ggccgaggac
     5941 cggcggcacg aaatcaaggc cgtagcggtg gttcacaatg aaacgtctac gggtgtgacc
     6001 acccggatcg cagaggttcg caaggcgatg gaccgcgccg ggcatcccgc gttgttgatg
     6061 gtggatacca tttcctcact cgcctctatg gagtaccggc acgatgaatg gggcgtggat
     6121 gtcaccgtgg ccggatcgca aaaaggattg atgctgcctc caggcttgag tttcaacgca
     6181 attagcgaaa aagcgctggc agcgtccaaa tcagcccgcc tgccgaaatc ctactgggac
     6241 tggcaggcga tggcgggtcc caataagagc ggttttttcc cctatacccc tggaaccaac
     6301 cttctctacg ggctgcggga ggccctgacc atgttgcgcg aagaagggct ggaaaacgtt
     6361 ttcgcccggc atgcccgcca cggcgaagcg acccgccgcg ctgtgcgggc ctggggcctc
     6421 gaagtgctct gcttgaatcc ggacgaatac agcagctcgc tgactgctgt gctgatgccg
     6481 gccgggtaca atgcggacga gctccggaag gtgattctgg aaaatttcaa catgtctctg
     6541 ggaaatggcc tgagcaagct tcagggcaag gtttttcgca ttggccatct gggcgatttc
     6601 aatgacctga tgctggctgg aacgctaagc ggcgtcgaga tgggcctggc gctggctggc
     6661 gtgcctcatt ctaagggggg catatcggcg gcgctcgaat atctcgccca ggctgcgagc
     6721 gaaactcgcc gccagccggt ggaagccact gtcgcctgac acgagcccag gcgagaggct
     6781 tgccgtgaaa cccaccgaac tgcaagtact ctatttgaca acgaccggcc gcaagaccgg
     6841 cttgccgcgg cagattgaga tctggtttgt ggccgctgat ggcaagctgt atctgctggc
     6901 ggagcatttt cgaaaagcga attgggtgcg aaacatcgag caagacccgc gtgtccgcgt
     6961 gcgcctcggc gaaaaagaat tcgccgcgac agcccgcgtg ctggacgaag agcacgaccg
     7021 cgacagctgg cggctggccc agcggttggg ccgcgaaaaa tacggctggg gcgaaggact
     7081 gccggtggag atcactccct ccgaaccgtt gtaaatcgcc gactttgaac attgattgaa
     7141 ccacgttctt tggcgaaacc atccactttc accatgcgcg ccgccacact cgccggcgtg
     7201 gaaactcgta gagcagcggc gcggcgtgtt ttcccgaccc ggctatcgag acgaacaagc
     7261 gcccgacgcc cgcttccacc cgacccaacc atcagcggtg gcgctggacg tctgggccac
     7321 gagattttcg cttcgcgctg cccatgagat gcgcttgttg atcggcgccg ggaagtaacc
     7381 aggatcacct cacccggcgc aggcggtaaa tgcggaacga atcatcttgg tacaccagca
     7441 gcggatgaaa cagccggtcg atcacgtgca tatggtgggg ttcggcccag gcgttggaca
     7501 ccagcagata ctgataccgc atggtctcga gttccccgcg aatctggtcc aaagaatagt
     7561 tgccgccggc gtcgtaatag cagcggaacc cgacgtcagc gtagaaactc gagcagtttc
     7621 ctaccgagta agcccgctcg ccgggctgcg ccaggcggtt caaggcagcg atcgaagggt
     7681 aggtgaccag agttttgcgc agaaactggt cggggttgat ccgccgggcg aatagttcaa
     7741 gccgcggtac attgatctgc agaatcagga ccagagtcag agaaaaggcc aggcaataag
     7801 ccgcaagcgc caagacgcaa tatcgccgga aagcatcgtt tcgatcggag tgaaaacggc
     7861 aaacacggtc tccgaggcag gcgaagagcg ctccgaaggc ggcgatcgca taccggacct
     7921 tgatcaaaat cgctgcccaa taaagaaaat atatggcgca aaataccagt acaagccggg
     7981 cggctgggga tgagttgcgc cttggccaaa acagcagcgg aagaaacgcg atgacgacga
     8041 acccgcacgg gttcggcgag ggagattcga aggcgggcaa gccgcgaaag tggatgcgcc
     8101 acggaaccga aagatagatg cccagacggt cgaggcgagt caattgggga tgcccggcag
     8161 cggttgtgga ccggaccgcg cttcgggtgg cttccggata gagcggattc ccggtaagaa
     8221 tcaaagtccg cacgtgccag taaagaccgg cactgaagaa tactgccgcc aaagcggcgg
     8281 cttgccgggg cctgagacgg aaagagcgcc agttccacaa cacaatcaaa gcccacggaa
     8341 ccgccccgag aatcgccaca tgtttcacgc caaaaccgaa acccaggaag agcgccgctg
     8401 cggcaagcca gccgaaccgc ctcgtttccg tccagcggac cgcgccgtaa agcgccagca
     8461 gcagatacaa cgccagagcc agatcattct tggcgacgct gcccgtccag tcaataaatg
     8521 gtgtagtcgc gaaaaaagcc atcgctgcca gagctccggc tcgagactcc gcgaaccagc
     8581 gctggccgat catggcgacg cttgcggcgc taagcgggaa aaaagcgtaa gtcagcgctt
     8641 tggccgtaat ctgcccttcc agcagaaagc cgagggtgta aagaatctcg atcccttgcg
     8701 gaaagtaaga gtacgggaaa taagggacaa tttccaggcg gtgatgcagc gcgtagtcgc
     8761 gcgccagtac gaagtgaaag ctcacaggat cgaaagcgat ttccgggctg agcacgacca
     8821 tggcgctcgc tatcatcgag acaaacagga agatcaggca cagcccccac aggggatccg
     8881 cggcagcgga cgggtacttc tcggccgcgc ggcgcagcag atcccacagg gtcttcgcgt
     8941 tcgcccggag caacagcgcc cccatgatca gcagagcgag cacggccgag cggtagagca
     9001 gatgcaacgc ccccagggcg aacaacaggg tgctcataat gcccaggccg gcggcaggaa
     9061 acagcgtcaa ctgttctagc gcggactccg ccagccggcc gagcgtcagg cggcccaaac
     9121 caaagcagac aaacagtgtc agtagtgaat acagtgtggg acggaaggcg taaaagagaa
     9181 aaaacgcgag aaaaaaagcg aagaaaatgc gcggttccca ggccgtcagc gctggccgtg
     9241 attgtctcaa tcggatgtat aagaagctgg cgacgatcag caaggcgaga agggccagat
     9301 atgccgcaaa aaattctttg ccgggatgcc ggtaatactc gagatgccag agaaagggcc
     9361 ttgcagtcac cgcaatcgta aacaggaccc acgccggcca caagatccgc gaggctattt
     9421 tcggcatagg agttgaaggc gcggcccgat ccaacgcagc aggccggaag atcccggcgc
     9481 gcggcgcagc cgattgtaga gactttccac gggcgcaaac cagcgggtat tcgatacgcg
     9541 tccggcaagc ggcgctggcg ctgccgcgca atcctcaaag ccggctttcc tcacccattg
     9601 gtccagctcc cgggcggatc gcaacacgat attgcgatat gcgcgtccgg aaacaaagcg
     9661 cacgtagccc gcttgcatag cgcgcggcag ccacccgacg ccccatatgc cggcgtgagg
     9721 ttcgggcgcc agggcgaaac ggttgttggt tgaaatgtag acggccccgc cggaagcggc
     9781 tactctatag cactcggcga acacgcgagc gggttcggcg acatgctcga tcaaatccgt
     9841 cgcggtgatg accgtgaagc ggccgcaggg gaaaggcaga agctcggcgt ttgcgcagac
     9901 gagctgagcc tgcactccgg cttcgcgaag ccgtgctgtc ccgattacga gccatcgaaa
     9961 ggccacatcc acgcccacaa gcgcggccag ccggcggccg gcggctgcga tgagcaggcc
    10021 gccggtcgag cagccaacgt cgagcagcgc gccgtctgga gaactttccc cgatttccgc
    10081 cagaacttgc tccccaatgg ctggctcggc cagcgcatgc gcggtgaatt tgcgcgccag
    10141 cggcggcggc acttccggcg tgatcgagta ataaaaatca agcatctcgt ggaaactgcg
    10201 gcggctggtt tccgcgaaga gccgctggcc tttgcggcgg tcgtcctcga tcgagatata
    10261 gcggtcggga agaatacgga aatcgggaat gccgcacagg accgggtact ccttccggca
    10321 gcggccgcag cgataggcgc tggcagcaaa gtccacgggg cctttgcagg ccgggcaggc
    10381 gaagggaatc acgcccattc ccgcagtgcg ccgcgaagcg ccaggtagcc ccgcacgctg
    10441 ggctcgaaga gacgccggat caggaaggga tagcggaggt agtaaatatc cagacgctcc
    10501 agcgcttccg ggcggctttc ggctgtgacc cagccgagcc gcgtcgagca accggcggcg
    10561 aagtggcgcg cgacgatcct ctgcgcgggc tcgggcatgg agccgtaagg ataggcgaaa
    10621 gcggaaaccg gccgacctgt ggcctcctcg atgcgctgct tcgaggagag aatctcctcc
    10681 cgcatggctg tttcctccaa ctgggccaaa ttcgtgtgcg tcgccgtgtg agcgccaaat
    10741 tcgattcccg cccggcttag ttccacaatt tgcggccagc tcatcagcgg caggcgtgga
    10801 atgtcgggcg gctgcgaggg ccaatcgttg gcgcctccgc aatagtttgt caccagaaac
    10861 accgttgccg ggaagttgta ccgggccagc accggcgcgg cggactcgaa gaaattttgg
    10921 aacccgtcat caaaagtgag cgccagagcc ggagcgggcg cagccggcaa ttccgtcagg
    10981 cggaccacgt tgacgccctg ccctgccagt tcctccatgt ggcgggcgaa gacactgggg
    11041 cgaacagaga tgaccgaacc ggaatcatcc agcgagtgat acgtgagaat ggcgcgccag
    11101 gagtccacgc ttgtgaccgt caacgagccg cagggaaaga cgagcatggc gggaagtcgt
    11161 ctccggccgg ctgacggaat gcgaatggtt cagaaccggc tgcccccata tcaatccgtt
    11221 tcggaacaag ccgctcccgc tccggcgatg tagcctgcga cctcgcccca gaaccaaacc
    11281 aaatagcata gcagcagcgc cggagaggca acgaggaact cccggcggcg gcgctttttg
    11341 gcctggatca gccgcatgga gcgaagcatg agcagcgcgg gaatcagcgg agaaaaaaga
    11401 atccgccgca tgcgcccaaa gccggtgagc gtgcgtgcga tcatgccccc gaagcagcgc
    11461 gcatgccggt agcgcagtgc gagataaggc gcgaagcgat acggtttacg atgaatgacc
    11521 gtcagacggg gatcgagcca caaaatatag cccagggcaa gcagctcgcg attcacgtca
    11581 ttctcccaaa agccgcccgc cagcagaggc gcgcagcgct ccagagcctc tcgcttataa
    11641 caagcgttgt tgccaggcag cgatgccgca ggcccggagt ccagcggcgg catagagccg
    11701 agatagcgcg aaaaataaat ggcccaatcc aagcagccgc cggcgcggcc attttccacc
    11761 ggcccgccca ccgcggcgat ttcccgggtc ctgtgagcgt cgatcatgcc cgccgcccac
    11821 cctgggggaa aagagcagtg atcttctgtg atcgcgatga gcttgcctcg cgagagacgc
    11881 acgccttcgc cgcgcagctc cggaacagag cgccggcccg gcatttcgat gatccgcagc
    11941 ttcgggtcgc aggccagttc cctgagcgcc tcccgcgtgg gatcggtcga agatacgacg
    12001 gcaataattt ccgccgcgat cccttcgcac tgctccagca gagctttgag tgtggaattg
    12061 atagtttgcc gggcgaagcg gaccggaaca atgatagaaa tttcaggtgc agccggcgtc
    12121 atcaaggccc tgaggacact cccggaggcg cgccggccag tttccattgc ctctgctgat
    12181 c
//
LOCUS       DASREK010000152        12135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2220535, whole genome shotgun sequence.
ACCESSION   DASREK010000152 DASREK010000000
VERSION     DASREK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220535"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..2397)
                     /locus_tag="VFA54_07565"
     CDS             complement(55..2397)
                     /locus_tag="VFA54_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZS50701.1"
                     /translation="MQSQRLSSAADQSLLDTRAADGRPQLPGAPLNHCPGEAPEPAPE
                     FCLRDYWRVLHAHRWRILLLCGGMALAACLFNYLEAPVYRSTVTLQIDHEQPNTTRLS
                     ETLVQPLDQPDYIETQYKILKSRTLAKSVMAKLNLADRAEYRRSWYQQELDPPRRGQD
                     LNPPVVRRFLDAITVSPGKGTRLVDLSVDSLDPEVAARAANALADAYIEQNLAAKWEA
                     TQKASEWLTRQLAELKAKLERSETDLLDYATQHSIIFVEEQKNITTLKLKQLEEELTR
                     AEAARIEKQSAYLLLQDSAERRAPLPGNLHSDTYRELSLKLAEAERDYAQLLVTFAPG
                     YPKAQRVKSQIDLLERSLAGERDRIIEGVRNDYLAAAEREKMLRNLVIRQTRSVNLLG
                     DDIIQYNILKRDADTNRQLYEGLLQRLKEAGVSAGLRASNIRVLDPAEVPERPYRPQK
                     LRNLVLALAAGLFAGVGLAFLSEHLSTLVRTPEEVEKLTGLSLLAVVPRTRSRLESFG
                     RSAFVFADDHCTPNGHPARAPASQRLPSGSQNSAGTGPASPARPKWQPEAALAEAYRT
                     LRSSLLLGWGDSMRRILITSPQPRDGKTTISVHLAWSLAQLGRRVLLVDADMRKPNCA
                     RQLGVGFEKGLSEYLEGAAESSEIIRETCVENLWVVPAGRPHPAASDLLHSARLWALL
                     YDAGTRWDHVVIDSPPSLALSDARTIAQLVEGVVLVVGDGTDRASLKRTQLAFDAAGI
                     RLLGFVMNRVNLDDMNYGYYREYGNYYYDSASHSAAGRSE"
     gene            complement(2457..3365)
                     /locus_tag="VFA54_07570"
     CDS             complement(2457..3365)
                     /locus_tag="VFA54_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZS50702.1"
                     /translation="MHRKWLLSGLLAAGFAIASEGPAVLPEKNLAATADYRLGPGDVL
                     EITIFEVEELSKPAVIAPDGAAVLPLIGAVKLSGLTPREAAARLVALYGNGLLRNPQI
                     SVTVKEYHSQPVSVLGAVGKPGIYQLRGFRKLSEVLALAGGLAADSGSVITISRPAET
                     ISVATRDLLRSPGDREDGPWVQGGDMIRVSKADVVYVAGEVARPGGFPLKDQQQITVL
                     KAISLAEGLRRDAAPQKAKILHNSGGMRQETPVKLLDILNGRGPDVALAPDDILFVPN
                     SRAKRAMARAAEAAIQVTTGVIIWRR"
     gene            complement(4349..5110)
                     /locus_tag="VFA54_07575"
     CDS             complement(4349..5110)
                     /locus_tag="VFA54_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HZS50703.1"
                     /translation="MNDKPLLRDERPLAIVTGASRGIGRAVALELARTHHIVATYRTR
                     ESEAAGLRDQCGAEIFRLDLASRDDREALISFCRQKWGRLDLLVNNAGMAPRSRVDLL
                     EAAEESFDEILAANLKGPYFLTQAAARWMQKQQAGRIIFITSISADTSSPNRGEYCLS
                     KAALSMAVKLFADRLAASGIPVFEIRPGIIRTDMIAPVAAQYETKIAAGLLPQGRMGE
                     PRDVALAVRAIADGLLDYSTGQVIHVDGGFSLRRL"
     gene            complement(5148..5669)
                     /locus_tag="VFA54_07580"
     CDS             complement(5148..5669)
                     /locus_tag="VFA54_07580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZS50704.1"
                     /translation="MQSILAESVLGGVVVAGAVTDLQTGKIPNWLTMPAMAAGLVISS
                     FAGIGLAKALYGLLAGLGVYFVFFAIGFRGAGDAKLMGAVGAFVGWPQVIVVMVVVAL
                     LGAVAALGLALRQGILAPVLSNTASLAADILRLRWREVQQRSDFRAPGSLRMRHGPVI
                     AAGTLLVILFHPA"
     gene            complement(5677..6075)
                     /locus_tag="VFA54_07585"
     CDS             complement(5677..6075)
                     /locus_tag="VFA54_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZS50705.1"
                     /translation="MAVAFFEERFLNKVIVRNKTRHRLLGDAIERADTSQTRRMGLLK
                     RTGLAKGEGLWILPCEGIHTFFMKFPIDVLFLNRKKQVVKALPSLGPWRLAMSLRGRS
                     VLELPAGTIRDTGTQRGDQIEIVERDVEQS"
     gene            complement(6129..6758)
                     /locus_tag="VFA54_07590"
     CDS             complement(6129..6758)
                     /locus_tag="VFA54_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HZS50706.1"
                     /translation="MQIRIRWRVRRKNFLYSCHYLFFFIGFAALGFCVLAYLDAALFQ
                     AYESRRFAKALKPAATSGRRIPGIAAVAAARDGSIIGKIEIPRLGVSAMVAEGVEPHT
                     LELAAGHIPGTSFPGQPGNVAIAGHRDTFFRNLRKIRTHDLIRLTTLSGSYDYSVESM
                     RVVGPDDVEVLKASGEPTLTLVTCFPFYYVGPAPERFVVRARQITGPAA"
     gene            complement(6807..7505)
                     /locus_tag="VFA54_07595"
     CDS             complement(6807..7505)
                     /locus_tag="VFA54_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50707.1"
                     /translation="MSRSKIFVSCLALLLFVFLPITRADEWNKKTIITFDKPVEVPGM
                     VLPAGTYVFRLADSQSDRHIVQILNKDENHVYATILAIPDYRLQPTGQTVVQFEERAA
                     NAPQAIQAWFYPGDNYGDEFVYPEVRARQIAQASSQPVLATKAPPQTPSEMKTAPVET
                     VRPETPQKQTEVAQAAPPPAPPQQTEQQPAAAPAQPAQPLPKTASPLPLIGLLGLSSL
                     GIGGVLRAMAKRSA"
     gene            7650..8348
                     /locus_tag="VFA54_07600"
     CDS             7650..8348
                     /locus_tag="VFA54_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZS50708.1"
                     /translation="MSALALYDCNRLRHWGPLLHFQKGENIFSQGDSADSLFRIHEGR
                     VKLTVVGAGGKEATVAVLAPGSFAGESCLADVERRTATATALIKCSVERINKAAGIRL
                     LHEDAVFREALLHGLLARNLEYEADLSDQVMNGSEHRLARALLKLCRLAGAERERITV
                     LSPKPTHEALAQMVGTTRSRVTFFMNKFRRLGFIEYDRALIVHADRISDFLRKDAARP
                     PTIESPPIEDAVLV"
     gene            complement(8353..8428)
                     /locus_tag="VFA54_07605"
     tRNA            complement(8353..8428)
                     /locus_tag="VFA54_07605"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8393..8395),aa:Met,seq:cat)
     gene            complement(8568..9692)
                     /locus_tag="VFA54_07610"
     CDS             complement(8568..9692)
                     /locus_tag="VFA54_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZS50709.1"
                     /translation="MIELRSRAQLRPQLETAFDFAQQQVKELVEKYPDFYPMYTVRGR
                     WRHQGEAWTHWCDGFLPGMMWIFYSRTREESWRQLAERYSRPLEPRRRDRSVHDLGFI
                     FMSTYHRWLQLTGEPGLGRVLIEAGRTLALRFQEKGGYLCSFLGPESLFIDIMMNVGI
                     VFYAAGRTGDARLAEIAARHSLTSRRVLVRGDGSTAHEGIFDTQTGAFLRQSTQQGLR
                     ADTCWSRGLSWALYGFGTAYAHTRDARFLETAQACADFYFQRTPPDGVPLWDYDAGPH
                     DPQWLDTSAAAIAASGLLQLADLAAGEARSILYREGALRILETLSSSLHLAAGCAGWE
                     GILRGGVYHIPKGLGVNESVMWGEYFFVEALGRALALLQV"
     gene            complement(9699..10964)
                     /locus_tag="VFA54_07615"
     CDS             complement(9699..10964)
                     /locus_tag="VFA54_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HZS50710.1"
                     /translation="MSLAFLLAAAVQAQQPAYDLLLKRGHLIDPKNNIDAPMDIAIGS
                     GKVAAVAADIDPARAAKVIDLGGLYVTPGIVDIHTHLYATSGMPDAWAGDNSVLPDGF
                     SFRSGVTTMVDAGSAGWRNFEMFRHTVIDRAQTRVFAMINIAGLGMMTDAAEQEESDF
                     RPEEVAKIAARNKDIVVGVKSAHYRHPDWTSVDRAIEAGRLSGLPVMVDFGYFLPQRP
                     YWQLVTEKLRPGDITTHVYRGSVPWIDEHGKLHAYLSRARARGVKFDVGHGGGSFVFR
                     NAVPAVRLGFYPDSISSDLHTLSMNAAMMDMPTTMSKFLAMGMSLPEVILRSTWNPAQ
                     IVHHSELGHLTVGAAADIAVWSIQRGDFGFADTGGGQFRGRERLFCEMTLKDGRIAWD
                     WNARSATDYRKLSPDYGIRRGIDAIVVPK"
     gene            11042..12130
                     /locus_tag="VFA54_07620"
     CDS             11042..12130
                     /locus_tag="VFA54_07620"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZS50711.1"
                     /translation="MSVNPEWNAQRVLEVLREVQEPELNLDLVSLQMIRDIDVSRGAV
                     ALTVALPTPGSPFQPQIEESIRKALAGAGATAVTVRWKTEIPEAHGASDKPPLPGVKN
                     IVAVGSGKGGVGKTTVAVNLAVGLASMGASVGLLDADVYGPNVPLMMGASGAPTAIED
                     RIQPLEQYGLRLMSMGFLSPGDKPLIWRGPMLHSVMQQFLKKVDWGQLDYLVIDLPPG
                     TGDVVLSLIQTVPVTGAVIVTTPSDVSLEDARKAVQMFNQVKVEILGIVENMSYLVCP
                     HCQERVDVFGHGGGKRTADRYGVPFLGEVPLNADVRLGGDTGMPVVLRDPQDPLAAAF
                     RSVGRRVAARLAVINLTSPAESILHVRM"
BASE COUNT         2435 a         3621 c         3753 g         2326 t
ORIGIN      
        1 ggggtcacgt gcggcggtga cggaattcgc cagccttgtg aatacaatga aaagtcattc
       61 agatctcccc gccgccgaat ggctcgcaga gtcatagtag taatttccat actcgcggta
      121 gtaaccgtaa ttcatgtcgt ccaggttcac gcggttcatt acgaagccga gaaggcgaat
      181 ccccgcggcg tcgaaggcca gctgggttcg cttcagagat gcccgatcag tcccatcgcc
      241 aaccacgagg accacgcctt ccacaagctg cgcgatggtc cgcgcatcgg aaagagcgag
      301 cgaaggaggc gagtcgatca ccacgtggtc ccagcgagtg cccgcgtcat acaacagggc
      361 ccagagcctt gcggagtgca acaggtcgga agccgccgga tggggccggc ctgccgggac
      421 gacccacagg ttttccacgc aagtttcacg gataatctcg ctcgattcgg ctgcaccctc
      481 caggtattcg ctcagccctt tttcgaaccc tacgccaagc tggcgggcgc agttcggctt
      541 gcgcatatcg gcgtccacca gaagcacgcg gcgtcccaac tgggccaggc tccacgcgag
      601 atgaacgctg atggtcgttt tgccgtcgcg cggctgtgga gaggtgatca aaatacggcg
      661 catggaatcg ccccatccga gcaacagcga gctccggagc gtgcggtagg cttcggcgag
      721 agcggcttcc ggctgccact tcggccttgc cgggcttgcg ggtccggttc ctgcgctgtt
      781 ctgggatcct gatgggaggc gttgtgatgc aggggcgcga gcgggatggc cattgggtgt
      841 gcaatggtca tcggcaaaca caaaggcgct cctaccaaag ctctccagcc gggagcgtgt
      901 tcgcggcacg accgccagca ggctgaggcc agtgagtttt tctacctctt ccggcgtgcg
      961 cacgagggtg ctgaggtgct ccgaaagaaa agccagtccc acgccggcga atagacccgc
     1021 cgccagcgct aggaccagat tgcgaagctt ttggggccgg tatggccgtt ccggaacttc
     1081 cgccggatcg aggactcgaa tgttggaggc ccgcagcccg gcggaaacgc ccgcctcctt
     1141 gaggcgttgc agcagaccct cgtacaactg ccggttcgta tcggcgtctc gtttgagaat
     1201 gttgtattga atgatgtcgt cgccgagcaa attcaccgag cgcgtctgcc ggataaccag
     1261 attccggagc attttttccc gttcggcggc ggcgagataa tcgtttcgga ccccctcaat
     1321 aatgcgatcc ctttcgccgg ccagcgaacg ctcgagcaag tcgatctggc ttttcaccct
     1381 ctgcgctttg ggatatcccg gggcgaaagt gaccaggagc tgggcatagt cccgctccgc
     1441 ttcggcaagc ttcaggctga gttcccgata agtgtcgctg tggagattgc ctggcagagg
     1501 cgcgcgccgc tctgccgagt cctggagcag cagatacgcc gattgcttct caatccgtgc
     1561 cgcttccgcg cgggtcagct cctcttcgag ctgtttcagc ttcagcgtgg tgatgttttt
     1621 ttgctcttcg acgaagataa tcgagtgctg cgtagcataa tcgaggagat cggtttcaga
     1681 gcgctccagc ttggccttga gctcggccag ttgccgggtg agccattccg acgccttttg
     1741 cgtcgcctcc catttcgccg ccagattctg ctcgatgtac gcatcggcga gcgcatttgc
     1801 cgcgcgcgcc gcgacttctg ggtccaagga gtcgacggaa agatcgacca gccgggttcc
     1861 tttgccgggc gagaccgtga tggcgtccag aaagcgccgg actacgggag ggttcaaatc
     1921 ctggccgcgt ctgggcggat ccaattcctg ttgataccag cttctacgat attccgcccg
     1981 gtccgcaaga ttcagctttg ccatcacgct cttcgccagc gtccgggact tgagaatctt
     2041 gtattgagtc tcgatgtaat caggctgatc cagcggttgg acgagtgttt ctgagagccg
     2101 cgtcgtgttg ggctgctcgt gatcgatctg aagcgtcacg gtcgagcggt ataccggcgc
     2161 ctccaggtaa ttgaacaggc aagcggcgag ggccattccg ccgcagagca agagaatcct
     2221 ccagcgatgc gcgtggagca ctcgccaata gtcacgcagg cagaactccg gcgcaggctc
     2281 cggcgcttcg ccggggcaat gattcagcgg ggcgccgggc aattgcgggc gcccgtctgc
     2341 ggcgcgtgta tcaagcaatg actgatcggc agcggaagag agtcgctgag attgcatagc
     2401 ggtgcgttcc gaagatgccg gcgagccgcg cttgaccctg gagtagcggg tcgacgtcag
     2461 cgcctccaga tgatgacgcc tgtagtaacc tggatagccg cttctgcggc gcgggccatc
     2521 gctctcttgg ctctcgaatt gggaacaaac agaatgtcat cgggggcgag agccacatcc
     2581 ggcccgcggc cgttcagaat gtccagcagt ttgacgggcg tctcttgtcg catcccgccg
     2641 gagttgtgga gaattttcgc tttttgcggc gcggcgtcgc gccgcagtcc ttccgccaga
     2701 gagatcgcct tcaagaccgt gatctgctgc tgatctttca gaggaaagcc gcccggccgg
     2761 gcgacctcgc ccgccacata cacgacatct gccttggaaa cacggatcat gtccccgcct
     2821 tgcacccagg ggccgtcctc ccggtcgccc ggtgaacgga gcaaatcgcg agttgcgacc
     2881 gagatcgtct cagccggacg gctaatggtg atcaccgagc cggaatcggc ggccagacct
     2941 ccggccaggg ccaatacttc agagagtttt cgaaagccgc gaagctggta aatcccaggc
     3001 tttccaaccg cgcccaacac cgataccggc tggctgtgat actcctttac agtcacggat
     3061 atttgcggat ttcgaagcaa cccgttcccg taaagcgcca ccaggcgcgc agcggcttcg
     3121 cggggagtca acccactcaa tttcaccgct ccaatcagcg gcaagacggc agcgccgtcg
     3181 ggagcgatga cggcgggctt gctcagttcc tccacctcga agattgttat ctcgagaaca
     3241 tctcctggcc cgagccgata gtcggccgtc gccgccagat ttttttccgg aagcacggcc
     3301 ggtccttcgg aggcgatcgc gaaaccagcc gccagcaaac cactcaacag ccatttgcga
     3361 tgcatagaca ctgaatgcat cggccttgca accgtagagc tttaggtacc ggcgggggct
     3421 agcgcgaaga aggcaccccc ccaatgtcat ccgggctgta tgagatcccc aatttggaaa
     3481 aacagcccgt agactatacc tcgctgggaa gttcggcgcc tcgatctacg aggcgatttg
     3541 gccatgacga cctgaacagg caacgtggga gcgcttttta ccgtcaagga agcgggaatg
     3601 gcaggcattt gccaaccttt tcactcaggc ggagttgatg aggcaggaat cgggtggacg
     3661 cgggctgacc gaagggcata attgcgagca gcaattcctg cgagcgtgcc agaagcggca
     3721 aagtttacga gcgcgaagct tttgcaagcg tttgcgcccg ggcgccggcg aatcgccgcc
     3781 cactgcgccg cgaaggctcc ggccccgagc gccgatttga tgaggatggt gcgctgtgtc
     3841 gaaagatgtc ccgaggagcc gcggagccag gggttgccct cccgatgtcc aaacgatgag
     3901 cgcagatcca cagcgttcgc cgccactacc gccgcccagc ttgcgatcca tcgctttcgc
     3961 cattttcgct gcggcatgtc tcggcagcgc gaggctttta cgatccccgg cgcgctgcct
     4021 tgttgggctc gcacgggaga tagggtgaag cttgataaca gagaaagacc tgccaggatg
     4081 gcaatggtgc ggtgatcccg tttttggacc ctgtgaatcc acatcggatt gttcggcgcc
     4141 ataagagccc agtgggccag cctctcgcct gccaactggg aacgttgatt gtaaattgga
     4201 cgggggcagc gcgctataac atggaagttt cattccgccg tggtacacgc cggtcagact
     4261 ttgtgctttg tactaggacg tttcggcaga gggccgccaa tccggcgcta cgcaacgtgc
     4321 tctgcggccg gctggaaaga agttcccgct agagccgccg cagagagaag ccgccgtcca
     4381 catgaattac ctggccggtc gagtaatcca gcaagccgtc ggcgatggcg cgcacggcga
     4441 gcgctacatc gcgaggctcg cccattcggc cctgggggag aagcccggcg gcgatttttg
     4501 tttcatattg cgccgcgacg ggggcgatca tgtctgtccg aataataccc ggacggattt
     4561 caaatacggg aattccggac gccgccaggc ggtcggcgaa cagcttgacc gccatgctca
     4621 gggcggcttt ggaaagacaa tattcgccgc gattgggcga ggaagtatca gcggaaatcg
     4681 aagtgataaa gatgatgcgt ccggcttgtt gcttctgcat ccagcgggcg gctgcctgcg
     4741 tgaggaagta tggccctttc aggttggccg ccaagatctc gtcaaaactt tcttcggccg
     4801 cttccagaag atccacacgg gaccgcgggg ccatacccgc gttgttgacc agcaggtcga
     4861 gccggcccca cttttggcgg cagaaggaaa tgagtgcttc acggtcgtcg cgggaagcaa
     4921 gatccaggcg gaaaatctcc gcgccgcatt ggtcgcgaag gccggcggct tcgctctccc
     4981 gcgtgcgata ggtggcaacg atgtggtgag tccgcgccag ttcaagggcg accgcccgcc
     5041 cgatgccgcg gctggcgccc gtgacgatgg ccagcggccg ctcgtcacgc aataagggtt
     5101 tatcgttcat gggcagttac ggcgcggcgc ggcgtccgac cggcggttca cgccgggtga
     5161 aacaggataa ccagcagcgt accggcggcg atcaccgggc cgtggcgcat tcgcaggctt
     5221 ccgggcgctc gaaaatcgct gcgctgctgg acttcccgcc agcgcaggcg caaaatatcc
     5281 gccgccaaag aggctgtgtt gctgagcaca ggagcaagga taccttggcg caacgcaagt
     5341 ccgagcgccg caacggctcc gagcaaggcc accacgacca taacgacaat tacctgcggc
     5401 catcccacga aagcgcccac cgcgcccatc agcttggcgt cgccggcgcc gcgaaatccg
     5461 atcgcaaaga acacgaaata aaccccgagg ccggccagaa ggccgtagag cgccttggcg
     5521 agtccgattc cggcaaacga acttatcacc agtccggcag ccatggccgg catggtcagc
     5581 cagttgggaa tcttgcccgt ttgcaaatcc gtaaccgccc cggccaccac tacgccgccc
     5641 agcacgcttt ccgccaggat gctctgcatg gaatactcaa ctctgttcga cgtcccgctc
     5701 gacaatctca atctggtcgc cgcgctgggt gccggtgtcc cggatcgtgc ccgcgggcag
     5761 ctccaggacg ctgcggccgc gcaagctcat cgcaagccgc cagggtccca agcttgggag
     5821 ggccttcacg acctgctttt tcctattcaa gaacagcaca tcgattggaa acttcataaa
     5881 aaaagtatga atgccttcac aggggagaat ccacagaccc tctcccttcg ccaggccagt
     5941 ccgtttcagc aggcccatgc ggcgagtctg gctggtatcg gcccgctcga tggcgtcgcc
     6001 cagcaggcgg tgccgggtct tattacggac gatgaccttg ttcaaaaacc gttcctcaaa
     6061 gaatgcaact gccaaggaat atacggtagc actaacggct gaaaaaagac tccgagtcct
     6121 gagccggatc aggccgccgg gcctgtgatc tgccgggccc ggactacaaa acgctcgggc
     6181 gcaggtccta cgtagtaaaa cgggaaacag gtaaccaagg taagggtcgg ctcaccggag
     6241 gctttcagga cttctacgtc atcggggccg actacacgca tcgattcgac cgagtaatcg
     6301 taagaaccgc tgagggtagt gagccgaatg aggtcgtggg ttcgaatttt ccgaagattg
     6361 cggaagaaag tatcgcggtg gccggcgata gcgacattgc ccggctggcc gggaaaagaa
     6421 gtgccgggga tgtggccggc ggccagttcc agggtatgcg gttcaactcc ctccgccacc
     6481 atggcagaaa cgccaagccg gggaatctcg attttgccga ttatggagcc gtcccgcgcg
     6541 gcggctactg ccgctatgcc cgggatcctg cggccggagg tcgcggcagg cttcagagcc
     6601 ttggcgaagc gccggctctc ataagcctgg aaaagcgcgg cgtccaggta ggccagaaca
     6661 caaaaaccca gagccgcgaa tccgatgaaa aagaacagat agtgacagga gtagagaaaa
     6721 tttttgcgcc gcacgcgcca ccgaatccga atctgcataa aggtcgcctg gcgcttttct
     6781 ggtcaccagc gccaggcgtc aatcgttcaa gcgctccgtt ttgccatcgc ccggagtaca
     6841 ccgccgatgc ccaaggagga aagtcccaac aagccgatca gcggcagcgg gctggcggtc
     6901 ttgggtagag gctgcgcagg ctgcgccggc gccgctgccg gctgctgctc ggtttgctgc
     6961 ggcggagcgg gtggaggcgc ggcttgagct acctcggttt gtttttgtgg cgtctctggc
     7021 ctcaccgtct ccacaggagc agtcttcatc tcagacggag tctgcggcgg ggccttagtc
     7081 gccaagaccg gctggctgct ggcctgcgcg atctgtcggg ctcttacttc cgggtatacg
     7141 aattcatctc cgtaattgtc accgggataa aaccacgcct ggatcgcctg cggcgcgttc
     7201 gcggcgcgct cttcaaactg cactaccgtc tgtccggtcg gctgaagccg gtaatccgga
     7261 atggccaaaa tcgtcgcgta cacatgattt tcgtctttat taaggatttg aacaatgtgc
     7321 cggtccgatt gggaatccgc cagcctgaat acataggtgc cagcgggcag aaccatgccc
     7381 gggacctcaa cgggcttgtc aaacgtgatt attgttttct tgttccactc gtctgctctc
     7441 gttataggca aaaatacaaa caagagcaac gcgagacagg agacaaagat cttactccta
     7501 ctcatttcgt tagtgctcct ttcaatttca gtctatcagt gtgagcggag aagtcagttc
     7561 aatattgaac atgcgcggga gcgtggccga atggattttg atgtaaccgt tctcacactg
     7621 cgcgcatcga aacagagagg ggttgtcaag tgtcggctct cgctctatac gactgcaacc
     7681 gcctgagaca ctggggacca ctgttgcact ttcaaaaagg cgaaaacata ttttctcaag
     7741 gggattcagc cgattccctg tttcgaattc acgaaggcag agtgaagttg acagtggtcg
     7801 gcgcgggagg caaagaagct acggtggcag tcctggcgcc gggcagcttc gccggcgaaa
     7861 gttgtctcgc cgatgtggag cggcgtacgg cgacagcgac tgctctgatc aagtgctcgg
     7921 tcgagcgcat caacaaagct gccggcatcc ggctgctcca cgaggacgcg gtttttcgcg
     7981 aagcgctgct gcatggtctt ctggcgcgca acctggagta cgaagcggac ctgagcgatc
     8041 aagttatgaa cggcagcgaa catcgcctgg cgcgcgccct tctgaaactc tgccggctgg
     8101 ccggcgccga gagggaaaga atcaccgtac tctctccgaa gcccacgcac gaggctctgg
     8161 cccaaatggt cgggaccacg cggtcgcgcg ttacgttctt tatgaataaa ttccggcgtt
     8221 tgggattcat cgaatacgat cgcgcgctga ttgtgcacgc agaccgcatt tcggattttc
     8281 tgcggaagga tgccgcccgc ccgccaacaa tagaatctcc accgatagaa gatgcggttt
     8341 tagtttgaag gttggtggcg gcggacggac tcgaaccgtc gacctaggga ttatgagacc
     8401 ctcgctctgc caactgagct acaccgccaa gcttttccta tcgtagcgat tcaaatggaa
     8461 gtctgtaaag acgccggacg ggccgacgag ggccaggctc ggcgtgcttc ctcccagcgc
     8521 tcggccgcgc caagagagcc atctctgccg tgaattttgc gtccaagtca gacttggagc
     8581 aaggctaagg cgcgtcccag cgcctctaca aagaagtatt ctccccacat cacggattca
     8641 ttgacgccca gtcctttggg tatatggtag acgccgcccc gcaaaattcc ttcccagccg
     8701 gcgcaaccgg cagccaggtg caaggagctg ctaagcgtct ccaaaatgcg cagggctcct
     8761 tcccgataga ggatactgcg ggcttcgcca gccgccagat ccgcaagctg gagcaatccc
     8821 gaggcggcga tggccgccgc cgaggtgtcg agccactgcg gatcatgggg acctgcatcg
     8881 taatcccaca aaggcactcc gtcgggcggc gtgcgctgga aatagaaatc ggcgcacgcc
     8941 tgcgcggtct ccagaaagcg ggcatcgcgg gtatgcgcat aggcggtgcc gaaaccgtag
     9001 agcgcccatg acaagccccg ggaccagcat gtatcggccc gcaacccctg ctgtgtggac
     9061 tggcgcagaa aggcgcctgt ctgagtatca aaaataccct catgagccgt gcttccgtcg
     9121 ccccgcacca ggacccggcg cgaggtcagc gagtgccgcg cggcaatctc ggcaagcctc
     9181 gcgtcgccgg tgcggccggc ggcgtaaaag acgatgccga cgttcatcat gatgtcgatg
     9241 aacaaagatt ccggccccag gaaagagcag agatatccgc ccttctcctg aaaccgtaaa
     9301 gccaaggtgc ggccggcttc gatcagcacc cggccgaggc cgggctcgcc cgtcagttgc
     9361 agccagcgat gataggtgga catgaaaata aaaccaaggt cgtggacgct gcggtcgcgc
     9421 cgccggggtt ccagcgggcg ggaataacgt tccgccagtt gtctccagct ttcctcgcgc
     9481 gtgcggctat agaagatcca catcatgccg gggaggaagc cgtcgcacca atgcgtccag
     9541 gcttcccctt gatgccgcca gcggccgcgg acggtgtaca tgggataaaa atccgggtat
     9601 ttttcgacca gctcttttac ctgttgctgc gcaaagtcaa aggccgtctc gagctgcggc
     9661 cggagctgcg ctctggaacg gagttcgatc atatccggct atttcggtac gacaatggcg
     9721 tcgatgccgc ggcgaatgcc gtaatcagga ctcagcttcc gataatcggt cgcgcttcgg
     9781 gcgttccaat cccaggcgat tcggccatcc ttcaaggtca tttcgcagaa caggcgttcc
     9841 cgcccgcgga actgacctcc gccggtatcg gcaaagccga aatctccgcg ctggatgctc
     9901 caaacggcga tgtcggcggc agctcccacg gtcaggtgcc ccagttcgga atggtggacg
     9961 atctgcgccg gattccaggt ggatcgcagg ataacttccg gcaaggacat gcccatggcc
    10021 agaaatttgg acattgtcgt aggcatatcc atcatggcgg cgttcatact gagcgtgtgc
    10081 aagtcgctgg agatcgagtc cggataaaaa cccagccgga cggccgggac tgcgttgcga
    10141 aagacaaagc tgcctccgcc gtgcccgacg tcaaacttca ctccgcgcgc gcgggcccgg
    10201 ctcaaatacg catggagttt gccgtgttcg tcaatccagg gcacagaccc gcggtagacg
    10261 tgcgtggtga tgtcccccgg ccgcagtttc tcggttacga gctgccagta agggcgctgg
    10321 ggcaggaaat aaccaaaatc caccatgacc ggcaggccag acaagcggcc cgcctcgatc
    10381 gcgcggtcga cggaagtcca gtcgggatgg cggtagtgag ccgatttgac gcctacgacg
    10441 atgtctttat ttctggcggc gatcttagcg acctcctcgg gacggaaatc gctctcttcc
    10501 tgttcggccg cgtccgtcat catgcccagc cctgcgatgt taatcatggc gaagacgcgg
    10561 gtctgggccc ggtcgatgac ggtgtggcgg aacatttcaa agttgcgcca cccggcgctg
    10621 ccggcgtcca ccatggtggt gacgccgctg cggaagctga atccgtcggg cagcacgctg
    10681 ttatcacccg cccaggcgtc cggcattccg ctggtcgcgt acagatgagt atggatgtcc
    10741 acaatcccgg gagtgacgta aagtccgccc agatcgatga ctttcgccgc gcgagccggg
    10801 tcaatatcgg cggccacggc ggcgactttg ccggagccga tcgcgatgtc catgggggcg
    10861 tcgatgttgt tcttcgggtc gatcagatga cctcgcttga gcagcaaatc gtaggccggc
    10921 tgctgcgcct ggaccgccgc cgcaagaagg aaggctaaag acatgaatga gttcctcaga
    10981 tggctcatgg tttgttccgt cgcgattgta gcgcggatgg gctgatatac tgaagtcgat
    11041 tatgagcgtc aacccggaat ggaacgccca gcgcgtcctc gaagttctgc gagaggtcca
    11101 ggagccggag ctgaacctgg acctggtgag tcttcagatg atccgcgaca tagatgtttc
    11161 gcgcggcgcc gtggctctga ccgtggcgct gccgactccg ggcagccctt ttcagccgca
    11221 gatcgaggag tccatccgga aggcgttggc gggcgccggc gcgacggcag tcacagttcg
    11281 ttggaagacg gagattccgg aggcgcacgg cgcctccgat aaaccgccgc tgccgggcgt
    11341 caaaaacatt gtcgcggtcg gctcgggcaa aggcggcgta ggcaaaacca cggtcgccgt
    11401 gaacctcgcg gtcgggttgg cctcgatggg cgcgtccgtg gggttgctgg acgccgatgt
    11461 ttacggtccc aatgtaccgc tgatgatggg cgccagcggc gcgccaaccg cgattgaaga
    11521 tcgcatccag ccgttggagc aatacggcct gcgcttgatg tcgatggggt tcctgagccc
    11581 cggcgacaag ccgctgatct ggcgcgggcc catgctccac agcgtaatgc agcagttctt
    11641 gaaaaaagtg gattggggcc agctcgatta cctggtcatc gatctgccgc ccggaaccgg
    11701 cgacgtggtg ctcagcctga tccaaaccgt tccggtgacg ggagccgtaa tcgtcactac
    11761 gccctcggac gtttctctcg aggacgcgcg gaaagcggtg caaatgttca accaagtgaa
    11821 agtggaaatt ctgggcattg tcgagaacat gagctatctg gtctgcccgc attgccagga
    11881 gcgagtcgat gtcttcggcc acggcggcgg aaagcgaaca gcggacaggt atggggttcc
    11941 ctttttggga gaggtgccgt tgaacgcgga tgtgcgtctc ggcggcgaca ccggcatgcc
    12001 cgtggtcttg cgcgatccgc aggatccgct cgcagcagct tttcgcagcg tggggagacg
    12061 agtcgccgcc cgcctcgcgg tgataaacct gacctcgccg gccgaatcga tcttgcacgt
    12121 tcggatgtga gccga
//
LOCUS       DASREK010000153        21774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2220727, whole genome shotgun sequence.
ACCESSION   DASREK010000153 DASREK010000000
VERSION     DASREK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 21774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21774
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220727"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFA54_07625"
     CDS             complement(<1..761)
                     /locus_tag="VFA54_07625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZS50712.1"
                     /translation="MLPLRFASDAPPIQVELWNGELLASARDAIGRVLIKTRRAFAEI
                     RRDPSLGFARGYAQGEIEIQGDLVGTLCAVLKSYLAWKTPRLVSKLARSFSPNTIRRA
                     RENAIAHYDLGNDFFGLWLDETMLYSGAIFTAEGVPLSQAQEAKCDLICRKLDLQAGQ
                     RFLDIGCGWGYLCLYAAKKYGVNSVGITLSPRQHGYGNARAARLGLGDRCRIELLDYR
                     QIAGSYDAIASVGMLEHVGRPYLKEFFQAVARALRP"
     gene            913..1170
                     /gene="xseB"
                     /locus_tag="VFA54_07630"
     CDS             913..1170
                     /gene="xseB"
                     /locus_tag="VFA54_07630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZS50713.1"
                     /translation="MPEAQQKLESFESALEQLEKIVKELENGDLPLEKSLELFERGME
                     LSENCRKQLSAAETRVEMLLKKQGKVEAVPFQLENSEGGER"
     gene            1278..2174
                     /locus_tag="VFA54_07635"
     CDS             1278..2174
                     /locus_tag="VFA54_07635"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZS50714.1"
                     /translation="MTAPALIQEHLNRQQRRIEEALERLTPAETEYPVSIHKAMRYSL
                     FAGGKRIRPILCLEAAAAVSDSTPGIETAASALEMIHTYSLIHDDLPALDNDDYRRGQ
                     PSCHKAFGEAIAILAGDALLTRAFQIVAEMETVPAERCVRIACELARAAGTVDGMIGG
                     QVADLEAEGKPVEEAHLHYIHRAKTAALIRAAVRVGGIYAGAADAQLDALSCFGAQIG
                     LAFQIVDDVLDVVASTEGLGKTAGKDARQQKATFPALYGIERSRAMALEAQQTAVNAL
                     ALFGDRADGLRGLARLIVERSA"
     gene            2171..2935
                     /locus_tag="VFA54_07640"
     CDS             2171..2935
                     /locus_tag="VFA54_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZS50715.1"
                     /translation="MKQKVRLDRLLVERGLAESREKAQALILAGQVLVAGQKAAKPGA
                     LVNGDAALRIMEGAALKYASRAGFKLEAALDHFSIDVQGKTCLDIGSSTGGFTDCLLQ
                     RGARRVYAVDVGKGQLAWKLRQDPRVVIREGINARCLQWQTIGEPVDLVTCDVSFISV
                     MLILPAIRQFLRRESRIIVLAKPQFEAGREQVGKGGIVRDPAIHAEVVEKVRRAVRDL
                     GFQRVEWLESPITGAEGNREFLVYGWQFSQQFHSGE"
     gene            2899..3777
                     /locus_tag="VFA54_07645"
     CDS             2899..3777
                     /locus_tag="VFA54_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZS50716.1"
                     /translation="MAIQPAVPFRRIGIISKPRRPDIREIAPALMQWIEARHLTYGYD
                     LETAQAIGASGGVTREELAATVDLLVVLGGDGTLLSAARAAGAREIPLLAVNLGGLGF
                     LMTTSPHELYSVLDCVLAGQFHIQCRTMVHGELLRGAELIGSYDALNDVVVNKAAMAR
                     VIDMDAYVDDEFVCSYRADGLIISTPTGSTAYSLSAGGPVIFPSVRALCITPICPHTL
                     TNRPLVLSETAQIEVVVKGADDASYLTIDGQVGVALRSHDRVRCSESKHRVHLIEPQR
                     LRFFEVLRSKMKWGET"
     gene            3774..4352
                     /locus_tag="VFA54_07650"
     CDS             3774..4352
                     /locus_tag="VFA54_07650"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HZS50717.1"
                     /translation="MKTLAMALMAAGFLQAQWVPLFNGKDLSGWKPTRNAVWKVENGI
                     LTGHWGAREHGGGWLLTERDFSDFILRVEFRGDPKQTNSGVAVRDPLHGAQNPTYSGY
                     EIQIHDNPEVPWPTGSIFGELRAKYLPLNDEGWNMFEITARGPLISVILNGEKVVEKN
                     DATRSLRGAIGVQSHDRDQTIYFRSIQIRELK"
     gene            4466..5383
                     /locus_tag="VFA54_07655"
     CDS             4466..5383
                     /locus_tag="VFA54_07655"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HZS50718.1"
                     /translation="MEDTLVALSNTLAEAVERASRYVAAVNARRHLSSSGVHWKQGVI
                     VTADHAIKREEEIAVTLPDGRSAPAKLAGRDPGTDLAVVTVEGAQFASVEIAPSESVK
                     PGILVLAVGRSAEAGVNATMGVVSSVSGRWQTWRGGMVDQLIRLDVALYPGASGGAVV
                     DVQGRLLGMATSGLSRMAGLAIPASTVSRVAEELLAKGHIVRGYLGLGLQRVALPDRL
                     TNVPQWPGSNAGLIVLTVERNGPADRAGVLIGDILLALGGKRLNEAGDLQAALGAETV
                     GNTIPAKILRGGVLKEIPITVAERLYRRG"
     gene            5385..6248
                     /locus_tag="VFA54_07660"
     CDS             5385..6248
                     /locus_tag="VFA54_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZS50719.1"
                     /translation="MPMERLGEVAETVRRSTVVIRAAQSPSAGSGVIWKPEGLIITNA
                     HVLPEPQVTVQLWNGSVHRAAVVSKDARLDLAALQIEAGALPAVASRDTKTVRVGELV
                     IAVGNPFGFIGAVSTGVVHAVGPLTIAGPLHVLTGRDWVQADVRLAPGNSGGPLADAS
                     GSLVGINTMVAGGLALAIPSNTVERFLRRGSSGASLGVIVRPVRVDNDSIGLLVLEVD
                     RHGPAAQASLLAGDVLTGADGRPFRSLDDLPRAIEQAHGGALRLHFFRGGRRWEREVS
                     VSLETKRVEAI"
     gene            6245..6904
                     /locus_tag="VFA54_07665"
     CDS             6245..6904
                     /locus_tag="VFA54_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS50720.1"
                     /translation="MIRAAILAASPVLSAGFQSLLRSSSAVEVVGAASDVDAFLQLME
                     DRQPEVIIADLASYEKAWPQLAAAPFKPAVIALMADDPQPQRTIDALRSGARAVITFD
                     IAPPSLEAVIQAAASGLIVIDPRIVKTLLSGDAGAASQASEEPEPLSEAGGLEPLTSR
                     EIEVLNLLGEGLGNKQIAGRLGISEHTVKFHVTSILGKLNAASRTEAVTIGVRRGLIL
                     L"
     gene            complement(7049..7969)
                     /locus_tag="VFA54_07670"
     CDS             complement(7049..7969)
                     /locus_tag="VFA54_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50721.1"
                     /translation="MSISPGSSGNFGGDAGPAWRPEPISEPRPKWFSLFVIIAVAALA
                     GANIYLFTQLASARQSLAQANQKIQAHDEQIAKLEGSVSRTYKEAEQGLMALKGEVSS
                     TRGQISQATKQVQTEVLSKTQQIAERLAAEQKAEVAKVGGEVEQLKTVASTTESKVGA
                     LSGEVSGVKTDVSSTKAELQRTIAELKSVRGDLGVQSGLVATNAQELAALKRLGERNY
                     FEFNIQKSKQPQRIGTISVKLRSTDTKRNRYTLDVIADDKLTEKKDRTINEPVQFYVA
                     KAKIPYEIVVNQVQKDRIVGYLATPKDQGR"
     gene            complement(8101..8952)
                     /locus_tag="VFA54_07675"
     CDS             complement(8101..8952)
                     /locus_tag="VFA54_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZS50722.1"
                     /translation="MIDLHSHTNKSDGTLSAVELIDLARVKGLRTLAITDHDTLEGYK
                     EGASYARQQGLDLVCGVELSTKFHGQTIHILGYFFHQQPGEAFCERLDSLQKARKDRN
                     RRLAARLRDLHIPIDLEEAEKLGRSQTGRPHFAQLLVQKGYAGSIKEAFDRYLDESAP
                     GYVERREVALEDVIRWIHEAGGLVSWAHPARMLTRSGLSVEAAFREMAGKTIDAVEAY
                     HSDHGPEQQAVYQAIATKLGLAVTGGSDFHGAAKPHIELGQVTLPAPALDVLRRLRPA
                     RPQNALP"
     gene            complement(9115..9726)
                     /gene="cysC"
                     /locus_tag="VFA54_07680"
     CDS             complement(9115..9726)
                     /gene="cysC"
                     /locus_tag="VFA54_07680"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF002059.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZS50723.1"
                     /translation="MQSKFHHEGFTIWFTGLSGAGKSTVAGILERRLRHLGARVEVLD
                     GDVVRTNLSKGLGFSKEDRDTNIKRIGFVCQLLSRNGVIAIAAAISPYRETRDFVRSQ
                     VQHFVEVFARCPLDKLVERDVKGLYKKALAGEIKEFTGISDPYEEPLHPEVIIDTHLE
                     TPEQSADKIWAKLEALGLITSYPASTLPFAQSGVQPRGTAVDN"
     gene            complement(9716..10474)
                     /locus_tag="VFA54_07685"
     CDS             complement(9716..10474)
                     /locus_tag="VFA54_07685"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HZS50724.1"
                     /translation="MIATAELSRLENATATDVLAWGIETFGDRLAVCTSFQAEGMVIL
                     DMAVRLNPAVRVFTIDTGRLPQQTYDLMTEVRQRYGVRVEVVFPDFRELEAMVGRHGP
                     NLFYEGVPRRMLCCEIRKVRPLDRKLRTLSAWVTGIRKGHSESRAGVRKVEVDAAHGG
                     ILKLNPLADWTAGQVEAYTREHQVPRHKLYSQGYTSIGCEPCTRATQPGEDLRAGRWW
                     WETDARKECGIHFSAQGKAQREVDVLLEDILSAI"
     gene            complement(10471..11583)
                     /gene="sat"
                     /locus_tag="VFA54_07690"
     CDS             complement(10471..11583)
                     /gene="sat"
                     /locus_tag="VFA54_07690"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HZS50725.1"
                     /translation="MHKPSNSNFGESLAPGSPVLPGTFKQLSDLFLLGEGGYRPVSRF
                     LTAHEVREVAETMHLPSGEPWSIPIIFPLSAEQAEALKKADRVVILDGSSPAGVIQIQ
                     EIFPLDKGHYARHVFRTEDTSHPGVAWLHGAGEFSIAGPVELFPGWKLQLPGGLPFAP
                     RDVRALIEQRGWKKVVGFQTRNPIHRAHEFVSKVALETADGLVIHPLLGETKADDIPA
                     EVRLACDRALVENYYSPQHTFIAGFPAWMRYAGPREAIFHAQVRRNYGITHLVIGRDH
                     AGVGKFYGPFDAQRIFSEFKPGELGVEPIFFDTVHYCRKCGGMTSPKTCPHGPEHHVS
                     LSGTAVRKMLSEGQTLPPEFSRPEVARILIEAGARA"
     gene            complement(11709..12068)
                     /locus_tag="VFA54_07695"
     CDS             complement(11709..12068)
                     /locus_tag="VFA54_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HZS50726.1"
                     /translation="MVIFKIGDLEVRSEWNQSQTARSILAALPLESTGNYWGGEFYFE
                     IPVKAPAEKDAQDVVERGTVAYWPAGRCLCIFWGPTPASQGDECRAASKVNVVGRVVN
                     VEALPKLKGRKVRVESK"
     gene            complement(12456..12965)
                     /locus_tag="VFA54_07700"
     CDS             complement(12456..12965)
                     /locus_tag="VFA54_07700"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HZS50727.1"
                     /translation="MFQKIFRKRNSLGAGAAVLALAGLLALGGAAYAQQAPAPQPARR
                     IDRVVDHMKTVLDLSDAQASQIRGILQQQMQAAQPLVDQQRQARLALRQAVVSGHTDT
                     ATLQPLANQIGQTASELALLRAQTAGQILPILTPDQQAKAKKLFQARGRFARHRGMIR
                     QQSVAPSQP"
     gene            13094..13786
                     /locus_tag="VFA54_07705"
     CDS             13094..13786
                     /locus_tag="VFA54_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS50728.1"
                     /translation="MPKILLVDDDTELCTLLDQYLRGQGFEVQAAHTGPEGLEAALEP
                     SVDLVVLDVMLPGFDGLEVLRRLRAKSHVPVLMLTARGEDIDRIVGLEIGADDYLPKP
                     FNPRELLARIRAILRRGEAAARPQGVLRVQDVELDPGARKVTRKGIPIELTTLEFDIL
                     EQLMKSAGRVLSRDQLMDALYERPATPFDRSIDMHISHLRRKLEAPQFGDRPLIKTIR
                     GVGYVFTREAGT"
     gene            13783..15141
                     /locus_tag="VFA54_07710"
     CDS             13783..15141
                     /locus_tag="VFA54_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS50729.1"
                     /translation="MKSSLFSKILLLLLATAFVAVAGFVAISAILGSFSPRAREPVVR
                     AVRFLAGEAVHAYEEGGEPALADMLRRIDTTYDFNGVLTDAKGTALTGPPEDFGPLVE
                     QAKKRRVFRAPLRRVLVFASEVQGRRGAYWFLLLVPSERFGFWFRFRQTLTAPHLWLL
                     LCILLLSYALARHISKPVQQLRLAAERLGLGDFTARVNSSRQDELGELARTFDKMAEQ
                     IEHAVTAQRQLLQDVSHELRSPLSRLAVAVELARSSSNPSEALDRVEREAARLNELVS
                     ELLAMTREELRPTEVNLAGLLEEVVEDVQIEAQARQCRVVLDGAPALIVSADSRLLQR
                     AVENVTRNAVYYTAPGTDVHVAAQRRNGGVEITVQDAGPGVPEAALTRIFDAFYRVDR
                     DRDRSTGGVGLGLSIARRAIEMHGGTITASNTHPGLRVVLRLPDSRVISEPAPSVMME
                     ET"
     gene            15138..15842
                     /gene="queC"
                     /locus_tag="VFA54_07715"
     CDS             15138..15842
                     /gene="queC"
                     /locus_tag="VFA54_07715"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZS50730.1"
                     /translation="MSGAEGTEEKPVAVVLLSGGMDSCVTAAVAAREHRLALLHSSYG
                     QRTEAREKQAFQQIADYYRAAFRLAIEQHYIGSIGGSALTDPSLPIPEGELRPPQSGP
                     IPITYVPFRNAHFLAAAVSWAEVLGARRIFIGAVEQDSSGYPDCRPEYYRAFNEVIRL
                     GVKPETHIEICAPLIHMKKADIIRLGLELGAPLSLTWSCYQSSEAACGVCDSCALRLR
                     AFAQAGVPDAIPYRRT"
     gene            15839..16441
                     /locus_tag="VFA54_07720"
     CDS             15839..16441
                     /locus_tag="VFA54_07720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HZS50731.1"
                     /translation="MKYLLLVCLAASCAAGEDLSFWIQPCSGQVAEDSRCQAADAELA
                     EWALHAWQAAAGGGIRFEKAAEEKRARIRIYWASGRAGLYGEAMPIRVDGKPGAAVYV
                     LPDVSQLGPAIAAAGREDALFRQAVVYLTCLHETGHAIGLPHTADYNDIMYSFAYGGD
                     ILEYFSRYRRKISVREHIRRNSGISASDQARLRKIYSDGR"
     gene            16535..17110
                     /locus_tag="VFA54_07725"
     CDS             16535..17110
                     /locus_tag="VFA54_07725"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZS50732.1"
                     /translation="MPEPIDFPLPPDLPRPLDDGAADHLPGAPMPSISLASTAGRMVD
                     LSGLTASRTVIYCYPMTGVPGKPLPEGWDLIAGARGCTPQTCGFRDHYQDLLQLRADV
                     FGLSTQTTQYQGEMAGRLRVPFEILSDAQFQLCDALRLPTFEVDGMRLLRRLTMIISG
                     GRIEHVFYPVFPPDENAAQVIRWLKDHPTAA"
     gene            complement(17221..17610)
                     /locus_tag="VFA54_07730"
     CDS             complement(17221..17610)
                     /locus_tag="VFA54_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZS50733.1"
                     /translation="MARAGALAEDRHKSRAEPPDGRPARGKAGEGRPLELDRLIHERM
                     RLGIVSALAVNDALSFNDLKRLLKTTDGNLSVHARKLEEARYITCAKYFAGRLPKTEY
                     RLTSAGRRALERYLDHMEALIRATREK"
     gene            complement(17603..18217)
                     /locus_tag="VFA54_07735"
     CDS             complement(17603..18217)
                     /locus_tag="VFA54_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50734.1"
                     /translation="MGSARPFPKARPEPIALEVRAMDNLRYIRETMESAASFTAVPGL
                     GGVVMGVTALLAAAIAVREPGAVTWLATWLGEATLAFLIGALAMVRKARAAKMPVLRG
                     PARKFALSLCPPIVVAALLTIVLYRAGLAGVIPAMWLLLYGTGVVTGGAFSVPIVPVM
                     GLCFMLEGAIGLFCPAAWGNWLMAAGFGGFHIIFGIIIARRYGG"
     gene            18296..18844
                     /locus_tag="VFA54_07740"
     CDS             18296..18844
                     /locus_tag="VFA54_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HZS50735.1"
                     /translation="MGMLSCKNRSVWRAWLRKNGGKRDEIWLLYYKNKSGQPSIGYED
                     AVEEALCFGWIDGKIKRIDEASYAQRFTPRRPGSRWSAINIRRAERMIAQGRMTAAGR
                     KAFRSGRRRESPRLPTRLPQPLQNRFELQKDAWENFQRFPPYYRRLTIGWIASAKKEE
                     TQLKRLAKLIDYSARHQRIKFM"
     gene            complement(18913..20289)
                     /locus_tag="VFA54_07745"
     CDS             complement(18913..20289)
                     /locus_tag="VFA54_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HZS50736.1"
                     /translation="MTRRKFLVSASAGVGSRLIAAPAKTPNIVFILCDDLGYGDLGCY
                     GSKIRTPNVDRLAREGVRFTNFCSADPVCSPSRAALLTGRYPTRVGVPRVLFPQDTGG
                     LNLDETTLADVLKARGYKTMCIGKWHLGRPPQFLPTSRGFDEYFGIPYSNDMNPRVLM
                     HNTEVIEQTADLASLTARYTERAERFIAQSNRSPFFLYMPHTFPHIPLAASPRFRGKS
                     QEGLYGDVVEELDWSVGEVLQSLRQKGLEKDTLVMFSSDNGPWYQGSPGKLRGRKNTA
                     YEGGVREPFIARWPGQIPAGHVCDALASMMDVFPTVTRLCGGILPPKPLDGIDIWPLL
                     TGRKKSIERPPLLYFDGWDLQCARWMNWKLHIARHNTAPYTPAPPGGRHSYLLSRPEL
                     YDLSSDPDESYDVAPENPQIIAQLQVKLAEMLKGFPEPVQKAYAEAQARKVNPATPAA
                     AYPKPAEP"
     gene            complement(20393..>21774)
                     /locus_tag="VFA54_07750"
     CDS             complement(20393..>21774)
                     /locus_tag="VFA54_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZS50737.1"
                     /translation="EVNRQFTKISWEARNPEALALMTRRAFKVAATEPGGPVYLAMAH
                     YALEAKGVKAQILPAERFMIRGRIRASAGAVQDAARMLIEARRPLVVVGDEVWKSGAQ
                     ANLLDFAEKLGLPVAAGVQGYANFPVMHPLYLGGFSMSSEYMRNADLLLVIGARDFGG
                     RVTPHSPEVPLEARIIRVGVNTSDMSRNYATDLALVSDVKESLNDLREAMGSMLTRER
                     MAAAAASRSGQVRALTAAMRARTEAEIRGNFGRNPMHPDEMGAILARTIDRDAIVVTE
                     NLTGKYDSFLFGFRPNEQMRLANTGNSLGWGIGAATGAKLGAPDRQVVCSIGDGSVMY
                     SASGFWTQARYGAPVLTVVWNNHNYQTVRFAYYNYKGKMASTGHYSGMYLGDPDIDFV
                     KLAQSQGVAGERVATARDLEPALKRGIAATRDGKPYLLDVIISRYGGGAESTWYQKFN
                     LAERRKRKV"
BASE COUNT         4159 a         6648 c         6673 g         4294 t
ORIGIN      
        1 ggccgcaagg ccctggcgac cgcttggaag aattctttca gatatggcct gccgacgtgc
       61 tccagcatgc ctacggaggc gatggcgtca taggaaccgg ctatctgacg gtaatccagc
      121 aattcgatcc ggcagcgatc cccgaggccg agacgagccg cccgggcgtt cccataaccg
      181 tgctgccgag ggctcagcgt gatgccgacg ctgttaacac cgtacttctt agccgcgtaa
      241 aggcacaaat agccccaccc acagccgatg tcgagaaagc gctggcccgc ctgcaagtcg
      301 agcttgcggc agattaagtc acacttggcc tcttgcgcct gactcaacgg aaccccttcg
      361 gccgtaaaga tcgctcccga gtagagcata gtttcatcca gccacagacc gaagaaatcg
      421 tttcccaggt cgtagtgggc tatcgcgttt tcgcgcgccc ggcgtatcgt gttgggcgag
      481 aaagaccgag ccagtttcga caccaggcga ggagtcttcc atgccagata gctcttgaga
      541 accgcgcaga gcgtccctac cagatcgccc tgaatctcga tttccccctg agcgtaacct
      601 cttgcaaatc cgagagatgg atctcgcctg atctcggcga aagcacggcg tgtcttgatt
      661 agcacccggc cgatcgcgtc acgggccgac gcaagcagct ctccgttcca caattccact
      721 tgtatgggcg gagcatcgga agcaaagcgg agcggcagca tcttaaggcc tcctgcacgt
      781 gggaaggtac ctttttccgt ctgatttcag cgtagcgcct gtgccagata ataatccgag
      841 aggcgccaat cgcaaagtgt ttttgcccac aggttagggc ccgctttcga ttacaataac
      901 gatggagact gcgtgcccga agcacaacag aaactcgagt cgtttgagag cgccctcgag
      961 cagctggaga aaatagtcaa agaactcgaa aacggcgatc tgcccctcga aaaatcgctg
     1021 gagctgtttg aaaggggcat ggaattgagc gagaactgcc gcaaacagct cagcgccgcc
     1081 gaaacacggg tcgagatgct gctcaaaaag caaggcaaag tggaagctgt gccgttccaa
     1141 cttgaaaaca gcgagggcgg cgagcgttag caccggctgc ggcgctttcc cgggcgcaac
     1201 cgtgggctcg ttctcagggc ggcaggccgg ccaactgccg gagcatatgg cggccgttcc
     1261 agactgctaa gaaatttgtg actgctcccg cgcttataca agagcatctt aaccggcagc
     1321 aacgccgcat cgaggaggct ctcgaacgcc tgactcctgc cgaaaccgaa tacccggttt
     1381 cgatccacaa ggcgatgcgc tacagcctgt ttgcgggcgg caagcgcatc cggcccatcc
     1441 tctgccttga ggccgccgca gccgtttccg attcgacgcc gggtatcgag acagccgctt
     1501 cggcgctgga aatgatccac acctattcgt tgattcacga cgatctgccg gccctggaca
     1561 atgacgacta ccggcgcggc cagccgagct gtcacaaggc ctttggagaa gccattgcga
     1621 ttcttgccgg cgatgcgctg ctgacccggg ctttccagat cgtcgccgaa atggaaacgg
     1681 ttcccgccga gcgatgcgtc cgcattgcct gtgaactggc gcgcgccgct gggacggtgg
     1741 acggcatgat cggcggccag gtcgcggatc tggaggccga aggcaagccc gtagaagaag
     1801 ctcatctgca ctacattcac cgcgccaaga ccgcagccct gatccgcgcc gctgtgcggg
     1861 tgggcggcat ctatgccggc gccgccgacg ctcagctcga cgctctgagc tgctttggcg
     1921 cccagattgg cctggcgttc cagattgtcg atgacgtgct ggatgtggtc gcctctacgg
     1981 aaggtctggg caaaacggca gggaaagacg cccggcagca aaaggcgact tttccggccc
     2041 tctacggcat agagcgctcg cgcgcgatgg ccctcgaagc gcagcaaacg gcggtaaacg
     2101 ccctcgccct gttcggcgac cgcgcggacg ggctgcgcgg gctcgcccgg ctgattgtcg
     2161 aacggagtgc atgaagcaga aggtccgcct cgaccgcctg ctggtggagc gcggcctggc
     2221 ggaatcgcgc gagaaggcac aagccctgat tctggccggc caagtactgg tcgcggggca
     2281 aaaagccgcg aagccgggcg cgctggtgaa cggcgatgca gcgctaagga tcatggaagg
     2341 cgctgctctg aagtacgcca gcagggccgg gttcaagctg gaagccgctc tcgatcactt
     2401 ttccatcgac gttcagggta agacctgcct ggatattggt tcctccacgg gcggcttcac
     2461 cgattgcctg ctccagcgcg gggccaggag ggtttacgcc gtcgatgtgg gtaaggggca
     2521 gcttgcctgg aagctgcggc aagacccccg cgtggtgatc cgcgagggca taaacgcacg
     2581 ctgcctgcaa tggcagacca tcggggagcc ggtagatctg gtaacctgtg atgtcagctt
     2641 tatttctgta atgttaattt tgccggccat ccggcagttt ttgcggcggg agagccgcat
     2701 tatcgttctg gccaagccgc agttcgaggc gggccgcgaa caggtaggaa agggcggcat
     2761 cgtgcgggat ccggcgatcc acgccgaggt cgttgaaaag gtgcgccggg ccgtgcgcga
     2821 tttgggattt cagagagtcg agtggctgga aagcccgata acgggagcgg agggaaatcg
     2881 ggaatttcta gtatacggat ggcaattcag ccagcagttc cattccggcg aataggcatc
     2941 atctcgaagc cgcgccgccc cgacatccgg gaaatcgccc ctgcgttgat gcaatggatt
     3001 gaggcgcgcc atcttaccta tgggtacgac ctggagacgg cccaggccat cggcgccagc
     3061 ggcggcgtga cgcgcgaaga gctggcggcg accgtagacc tgctcgtcgt gcttgggggc
     3121 gacggaacct tgctttccgc cgccagggcg gccggagctc gtgaaatccc gttgctcgcc
     3181 gtcaatttgg gtggactggg gtttttgatg accactagcc cgcatgagct ctactcggtg
     3241 ctggattgcg tgctcgccgg ccagtttcat attcagtgcc ggacgatggt gcacggcgaa
     3301 ttgctgcggg gcgcagagtt gatcggctct tatgacgcgt tgaacgatgt cgtggtgaac
     3361 aaagccgcca tggcgcgcgt catcgacatg gacgcctatg tcgatgatga gttcgtatgc
     3421 agctatcggg ccgacgggtt gatcatttct actcccaccg gctcgactgc gtactcgctt
     3481 tccgccggag gaccggtgat tttcccttcc gtacgcgctc tgtgcattac tccgatttgc
     3541 ccccacacgc tgaccaaccg cccgctggtg ctttccgaaa ctgcgcagat cgaagtggtc
     3601 gtgaaaggcg ccgatgacgc atcctatttg accatcgacg gccaggtcgg cgtcgccttg
     3661 cgcagtcacg accgcgtgcg ctgctcggaa tccaagcacc gcgtgcacct gatcgaaccg
     3721 cagcgcctgc gcttctttga agtgcttcga agcaagatga agtgggggga aacatgaaaa
     3781 cactcgcaat ggccttgatg gccgccggat ttttgcaggc gcaatgggtt cccctgttca
     3841 acggcaaaga ccttagcggc tggaagccaa ctcgtaacgc cgtctggaag gtggaaaacg
     3901 gaattctcac tggccactgg ggagcccgcg agcacggcgg cggctggctg ctcaccgagc
     3961 gggacttttc cgacttcatt ttgcgcgtcg aatttcgcgg agatcccaaa cagacaaaca
     4021 gcggcgtcgc tgtccgcgac ccgcttcacg gcgctcaaaa cccaacctac tctggctatg
     4081 aaattcaaat tcatgacaac ccggaggttc cctggccgac cggcagtatt ttcggcgagc
     4141 tcagggccaa atatctgccc ttgaatgacg aaggctggaa tatgttcgag atcacggcgc
     4201 gcgggccgct gatttccgta attctaaacg gcgaaaaagt cgttgagaaa aacgacgcca
     4261 cccggtcgct gcgaggagcc atcggcgtac aatcccatga ccgggatcaa accatttact
     4321 tccgcagcat ccagattcgg gaactgaagt aagtttactg gcccgcagcc cgatcgccgc
     4381 ctggactttt ttccaggccc ctgcctgccc aattgagcga tgtgcttccg ctcggctctt
     4441 ctgcatactg aatgcaggag gaagcatgga agatacacta gtggcgcttt ccaacacctt
     4501 ggccgaggcc gtggagcggg ccagccgtta tgtggcggcg gtaaacgcca ggcggcacct
     4561 ttcgtccagc ggcgtgcatt ggaagcaggg cgtgatcgtc acggctgacc acgcgatcaa
     4621 gcgggaggaa gagatcgctg tcacgctgcc ggacgggcgc tcggccccgg ccaagctggc
     4681 cggacgcgat cctggaaccg acttggccgt tgtgacggtg gaaggcgcac aattcgcttc
     4741 ggttgagatc gccccaagtg aatcggtgaa gccgggaatt ctggtgctgg cggtgggcag
     4801 atccgcagaa gccggcgtca acgccacgat gggcgtggtc agctcggtga gcgggcgatg
     4861 gcagacgtgg cgcggcggga tggtcgatca attgatccgt ttggatgtcg ccctctatcc
     4921 aggcgcgtcc ggaggcgccg tggtggatgt gcaaggccgc ctgctcggca tggctacatc
     4981 aggcctctcg cggatggctg gattggcgat tccggcttcg accgtaagcc gcgtggccga
     5041 ggagctgctc gctaaaggcc atatcgtgcg cggatacctc gggttgggtt tacagcgcgt
     5101 cgcactgccc gatcgtctga caaacgtccc gcagtggcca ggatccaatg ctggtctgat
     5161 agttttgact gttgagcgca acggccctgc ggaccgtgcc ggcgtcctga tcggcgatat
     5221 tttgctggcc ctcggcggaa agcgcctaaa cgaagccggc gacttgcaag cggcgctcgg
     5281 cgccgagact gtcggcaata caattcccgc gaaaatcctg cgaggcggcg ttctgaagga
     5341 aatcccgatt accgtggccg aacgtctgta ccggagaggc taagatgccc atggagcgat
     5401 tgggggaagt agccgaaacg gtgcggcgct cgaccgtagt gattcgagcc gcgcaatctc
     5461 cgagcgccgg ctccggcgtc atctggaagc ccgagggact cattatcacc aacgctcacg
     5521 tgctgccaga accccaggtc accgtgcaac tgtggaacgg gagtgtccat cgggcagcgg
     5581 tggtttccaa agacgccagg ctggatctgg ccgcgctcca aatcgaggcc ggggcgctgc
     5641 cggctgtggc cagccgggat accaagacgg ttcgagtcgg cgaactggtc atcgccgtag
     5701 gcaacccgtt tggcttcata ggagctgtca gcacaggcgt agtccacgcg gtcgggcctt
     5761 tgacgatcgc cggaccgctt cacgttttga cggggcgcga ctgggtgcaa gcggatgttc
     5821 gcctggcgcc cggaaactcc ggcggcccgc tggcggacgc gagcggctcc ctcgttggta
     5881 ttaacactat ggttgccgga ggactggccc tggccatccc cagcaacaca gtggagcgtt
     5941 ttttgcggcg cggttcatcc ggggcctctc ttggcgtgat cgtgcgtcct gtcagggtgg
     6001 ataacgatag cataggattg ctcgtgctgg aggtggatcg tcacggcccc gccgcccaag
     6061 cctcgctcct ggcgggcgac gtgctgaccg gcgcagacgg ccggccgttt cgctcgttgg
     6121 acgacctgcc gcgggccatc gaacaagccc atggcggcgc gctgcgattg catttcttcc
     6181 gaggcggccg gcgctgggag cgagaggtca gcgtgagcct ggaaacgaag cgggtggagg
     6241 cgatttgatc cgtgcggcga tcctggctgc ttcgcccgta ttgagcgccg gctttcagtc
     6301 cttgctgcgc tcgagttcgg cggtagaggt cgtcggcgcc gcctccgatg tagatgcgtt
     6361 cttgcagctc atggaagatc ggcaacctga agttatcatc gccgacctgg ccagctacga
     6421 aaaagcctgg ccccagcttg cggccgctcc gtttaaaccg gccgtaatag ccttgatggc
     6481 agacgacccc cagccgcaac ggaccatcga cgcgctgcgt tcgggagcgc gggctgttat
     6541 cacgttcgac atcgctccgc caagtctcga agcggtgata caagccgcag ccagcggtct
     6601 tatcgtaatc gatccgcgga ttgtcaaaac tctcttatcc ggagacgccg gagcggcttc
     6661 gcaagcctct gaggagccgg agccgctatc cgaggccggc ggcttagagc cgctcacttc
     6721 tcgtgagatt gaagtgctga atctgttggg tgagggcttg ggcaacaagc agatcgcagg
     6781 gaggctcggc atttccgaac acaccgtgaa atttcacgta acttcgatac tcggcaagct
     6841 gaatgcagcc agccgcaccg aggcggtcac tatcggagta cggcgcggct tgattcttct
     6901 gtgatggcca ttgtgggtgt cccaccgcag cgagcccggc ggccgcggat tcgctgattc
     6961 gctttctttg gcggtgtaaa gcagggccgc ggaatagacc aggaggctgg cgctgtcaat
     7021 gccttttcaa cgagccttcc agtcgacgtc agcgcccttg atctttgggc gtagccaggt
     7081 agcccacgat tcgatccttc tgtacctggt tgacgacgat ctcgtagggg attttcgcct
     7141 tggctacgta aaactgcacc ggctcgttga tggtgcggtc cttcttctcg gtcagcttgt
     7201 cgtcagcgat tacgtccagc gtatagcgat ttcgtttcgt atcggtgctg cgcaatttta
     7261 ccgagatcgt cccgatgcgc tggggctgct tgctcttttg gatattgaat tcaaaatagt
     7321 tgcgctcgcc gaggcgcttc agggcagcca gttcctgggc gttggtggcc accagcccgc
     7381 tctgcacgcc caaatcgccg cgaaccgatt ttagctcggc aatggttcgc tgcaattccg
     7441 ccttggtcga ggaaacgtcg gttttcactc ccgaaacctc gccgctcaga gcgcccacct
     7501 tggattccgt tgtggaagcc acggttttaa gttgctccac ttccccgccc accttcgcaa
     7561 cttcagcttt ctgctccgcg gcgagacgtt ccgcgatctg ctgggtcttg gagaggactt
     7621 ctgtctggac ctgcttggtg gcctggctga tctggccgcg agtcgatgag acctcgcctt
     7681 tcagagccat cagcccctgc tcggcctctt tgtaggtccg cgagacagaa ccttcgagct
     7741 ttgcgatctg ctcatcatgg gcctgaatct tctggttcgc ttgtgccaga ctctggcggg
     7801 cggacgccag ttgggtgaat agatagatgt ttgcgccggc aagggcggcc accgcaatga
     7861 tgacgaacag gctgaaccat ttcggccgcg gctcggagat tggctccggc ctccatgccg
     7921 gtcccgcatc tccaccgaag tttcctgacg atcccgggct gatactcacg gcatctctcc
     7981 taagagcaaa tttttaagca ttatacacta taaagaggat cgggccggcg cgggataggg
     8041 ctatctccga gtcagataag gaggaaattg ggtttcgacc gggcggcaag tccggcagcc
     8101 ctaaggaagg gcgttttggg ggcgggcagg ccggaggcgg cggaggacgt ccagcgctgg
     8161 cgcaggcaac gtcacctgcc cgagctcaat gtgaggcttc gccgctccgt gaaaatccga
     8221 tcctccggtt actgccaggc ccaacttggt cgcaatggcc tgatagacgg cctgctgttc
     8281 ggggccatga tcgctgtgat aggcttctac ggcgtcgatc gttttccccg ccatttcgcg
     8341 aaaagcggcc tccacggaca atccgctgcg cgtcaacatg cgagcggggt gcgcccagga
     8401 aaccagccct ccggcttcat ggatccagcg gatcacatct tcgagagcca cctcacggcg
     8461 ctccacgtac ccgggtgcgc tctcgtccag atagcggtca aacgcctcct taatgctccc
     8521 ggcgtagcct ttctggacga gaagctgcgc gaaatgcgga cgtcccgtct gcgaccgtcc
     8581 caacttttcc gcctcttcca ggtctatcgg gatatgcaaa tcgcgcagcc tggcagcgag
     8641 ccggcgattt ctatctttac gcgctttttg cagagaatcc aggcgctcgc aaaaggcttc
     8701 tccgggctgc tgatgaaaaa aatagcccaa gatatggatc gtctgcccgt gaaatttcgt
     8761 gctcaactcg accccgcaaa ccaggtccag gccttgttgc cgggcgtagg aagcgccctc
     8821 cttgtagccc tcgagcgtgt cgtggtccgt gatggccaaa gtccgcaagc ctttgacgcg
     8881 ggcaagatca atcaattcca cggcagagag agttccgtcc gacttgttgg tatgtgagtg
     8941 gagatcgatc atagccgctc ccgcctattg ggagcaggag ttcctagaat aaacgatcgt
     9001 cgatcatttc cgcgcagatc tgtttgtgtg tacgggggcc ggcggtccgc ttttctggcc
     9061 ctggcgccgg cggccaagcg ccgctatccc aaatgcggga agcgggattg gaaattagtt
     9121 atctacagcg gtcccgcgag gctgcacacc cgattgcgcg aagggaagcg tcgaggccgg
     9181 atagctcgtt ataaggccga gcgcttcgag cttggcccag attttatctg cgctctgctc
     9241 gggtgtctcc aggtgagtgt caatgatgac ttccggatga agcggttctt cgtaagggtc
     9301 tgaaattcct gtaaactctt taatttcccc ggccaaggct ttcttgtaca aacctttgac
     9361 gtctcgctcc accagtttgt ccagcgggca ccgcgcgaaa acctctacga agtgttggac
     9421 ttgggatcgg acgaaatcac gggtttcacg gtacggagaa atcgcggcgg cgatggcgat
     9481 cacgccgttg cgcgacagca actggcagac aaacccgatc cgcttgatat tcgtgtcccg
     9541 gtcttccttg ctgaagccca agcccttcga aaggttcgtc cggacgacat cgccgtccag
     9601 cacttcaact cgcgcgccga gatggcggag gcggcgctcg aggattcccg cgaccgtact
     9661 cttgcccgcg ccggacaggc ccgtgaacca gatcgtaaaa ccctcgtgat gaaatttaga
     9721 ttgcactgag aatatcctcc aggagcacat caacctcgcg ctgcgccttc ccctgagccg
     9781 aaaaatggat gccgcattcc ttccgcgcgt cggtttccca ccaccagcgc ccagcgcgca
     9841 agtcctcccc aggctgggtg gcgcgtgtgc atggctcgca gccgatactc gtgtagcctt
     9901 gagagtaaag tttatggcgg ggcacctggt gttcgcgcgt ataggcctcc acttggccgg
     9961 ccgtccagtc ggccaaggga ttcagtttta aaattcctcc gtgcgctgca tcgacttcca
    10021 ctttccgcac cccagcccgg ctctcgctat gccctttccg gatcccggtg acccaggcgc
    10081 tgagcgttct tagcttccgg tcgaggggcc tgactttgcg gatctcgcaa caaagcattc
    10141 gccgcggcac gccttcgtaa aacaggttcg gcccatgccg gccgaccatt gcctcgagtt
    10201 cccggaagtc gggaaaaacg acttccacgc gaacgccgta ccgctggcgg acctccgtca
    10261 tcagatcgta ggtctgttgg ggcagccttc ccgtgtcgat tgtaaacacc cgcacggcag
    10321 gattgagccg tacggccata tccaggatca ccatgccctc ggcctgaaag cttgtgcaga
    10381 ccgccagccg gtcgccgaac gtctcgatgc cccaggcgag cacgtccgta gccgtggcgt
    10441 tttcaaggcg cgacagttct gctgttgcga tcacgcgcgg gctcctgcct cgatcaggat
    10501 tcgggccact tccggacggg aaaactcggg cggcagcgtt tgcccctcgc tcagcatctt
    10561 gcgcacagcc gtgccgctca gggaaacgtg gtgttccggt ccgtgcggac acgttttggg
    10621 cgaagtcatc ccgccgcact tgcggcaata gtgaacggta tcgaagaaaa taggctcgac
    10681 gcccagttcc ccgggcttga actccgagaa aatccgctgc gcatcaaacg gcccgtaaaa
    10741 ttttccgacg ccggcgtggt cgcggccgat aaccaggtgc gtgattccat aattgcgccg
    10801 cacctgcgcg tgaaaaatgg cttctcgggg ccccgcgtag cgcatccaag ccggaaagcc
    10861 ggcgatgaag gtgtgctggg gcgagtagta attttcaacg agcgcccgat cacaggcaag
    10921 ccgtacttcg gccggaatat catccgcctt agtttccccg agcaaaggat gaatcaccag
    10981 accgtcggcc gtctcgagcg ccaccttgct cacaaactca tgagcgcgat gaatcggatt
    11041 ccgcgtctgg aatcccacaa ccttcttcca gccgcgttgc tcgatcagcg cccgtacgtc
    11101 gcgcggggcg aaaggcaatc ctccgggcaa ttgcagcttc caaccgggga acagttcgac
    11161 aggacccgca atactaaact ccccagcgcc gtgcagccag gctacgccag gatgcgatgt
    11221 atcctcggtc cgaaaaacgt gacgggcgta atgacccttg tccaatggaa agatttcctg
    11281 gatttgaatt acgccggcgg ggctgctgcc gtccaaaatc accacacgat ccgctttctt
    11341 cagtgcttcg gcctgctcgg cgctgagggg aaagatgatc gggatgctcc acggctcgcc
    11401 gctcggaagg tgcatcgtct cggccacctc gcggacttca tgggccgtca gaaaccgcga
    11461 aaccggccgg tagccgccct ccccgaggag aaacagatcg cttagttgct tgaaagtgcc
    11521 ggggagaacc gggcttcccg gcgcaagaga ttccccaaaa ttggagttcg aaggcttgtg
    11581 caatggtcct aatccctata gagatgcagt tatttagctt accacaaaca tgagatgact
    11641 cacaaccgga actggattcg taatattctt ctgcggaaaa aacgttgatt cggcgggcgt
    11701 tacagaagct acttcgattc tacgcggacc ttgcgcccct tcagctttgg cagcgcttcg
    11761 acatttacca cgcggcccac gacattcact ttgctggcgg cccggcactc atcgccctgg
    11821 ctggcgggcg taggccccca gaatatgcat aaacagcgcc cggcaggcca gtaggccacg
    11881 gtacctctct ccacgacatc ttgtgcatct ttttctgcgg gcgctttcac cggaatttca
    11941 aaatagaatt cgcctcccca gtaattgccc gttgactcca gcggcagagc ggcgagaata
    12001 ctgcgcgcgg tctgcgattg attccactcg gaacgcactt ccagatcgcc aatcttaaaa
    12061 atcaccatag atgtgcctga agaaatggaa caggggatct ccagccggcg atgcgccgag
    12121 tcccgcccgg ctttccgaac cccagccgat ctgcgtcccc tacgtaaacc aaagcataga
    12181 gcggcgcatt ccctgccccc ggcgcatgat agagcctgaa gttctacgaa gtcaagggaa
    12241 cttcggattg aaccatcatc gaactttgtg gcccgaattc gggggcgccg ggagcccgtg
    12301 ctttggcagc tccagcaacc caattccggc gatcgcagac ggtgggaggt tcaggtacca
    12361 gccgggcaaa gcaaaggctg ctgccgaaac gggaaaaccg ggcggtgcgg gcgcgcgcgg
    12421 ttctgaaacc aacgcgcacc cggcggcgat tgtgcttacg gctggctggg ggccacagac
    12481 tgctggcgga tcatgcctcg atggcgggca aaccggcctc tggcctggaa cagcttcttt
    12541 gctttcgctt gctggtccgg cgtgagaatc ggcaggatct gcccggcggt ttgcgcacgc
    12601 aagagggcta gctcagaggc cgtctggccg atctggttgg ccaggggctg caaggtcgcc
    12661 gtgtcggtgt ggccgctcac cacggcttgc cggagagcca ggcgagcctg gcgttgctgg
    12721 tccacgaggg gttgggcggc ttgcatctgc tgctggagaa taccgcgaat ctgagatgct
    12781 tgcgcatcgg aaagatccag aactgtcttc atgtggtcca cgacccggtc gattcgccgc
    12841 gccggctgtg gagcgggcgc ttgttgggca tacgcggcgc ctcccagggc cagtaagccc
    12901 gcgagcgcca agacagccgc cccggcgccc agcgagtttc gcttgcgaaa tattttctga
    12961 aacatagcct atttcctcct actgccagtt aacccggcct gattgagctc catttgtgcg
    13021 cgctgtaaag atttgtaaag ggctcgaatc cccggtttga tcgtgctaga agtgaaaaaa
    13081 gagggacgca gccgtgccca aaattctcct ggttgacgat gacacagagc tgtgcacgct
    13141 gctcgaccag tatctgcgcg ggcaagggtt tgaagtacag gcggcgcaca cgggcccgga
    13201 aggactggag gccgcgctgg agccctcggt ggatctggtg gtactcgatg tgatgctgcc
    13261 cggatttgac ggcctggagg tgctgcgccg cctgcgggcc aaatcgcacg tccccgtact
    13321 catgctcacg gcgcgcggcg aggatatcga ccgcatcgta gggctggaga tcggcgctga
    13381 cgactattta cccaagccgt tcaatccccg ggaattgctg gcgcggatcc gggccattct
    13441 tcgccgcggc gaggcggcgg cacggccgca gggcgtgctg cgcgtgcagg atgtcgagct
    13501 cgatcccgga gcgcgcaaag tgactcggaa aggcatccca atcgagctga ctactctcga
    13561 attcgacatt ctcgagcagt tgatgaaatc cgctgggcgc gtcctctcgc gcgatcagtt
    13621 gatggacgcc ctctacgaac gccccgccac gccgtttgac cggtccatcg acatgcacat
    13681 cagtcatttg cggcggaaac tcgaggcgcc gcagttcgga gaccgtcctc taatcaaaac
    13741 cattcgcggc gtgggctatg tgttcacacg ggaggcgggc acatgaaatc gtccctgttc
    13801 tcgaagattc tgctgctgct tctggccact gcgtttgtgg ctgtcgccgg attcgtggcg
    13861 atttcggcta tcttgggctc gttttctcct cgcgcacgcg aacctgtcgt acgggccgtc
    13921 cggttcctcg ccggcgaggc ggtccacgct tacgaagaag gcggcgagcc ggctctggct
    13981 gatatgctcc gtcgcattga cacgacctac gatttcaacg gcgtgctgac tgatgcaaag
    14041 ggaacggcat tgaccgggcc ccccgaggat ttcggccccc tggtggagca ggcgaaaaag
    14101 aggcgtgtgt tccgcgctcc tttgcggcgg gtgttggtgt ttgcatccga ggtacaggga
    14161 cggcgcggcg cctactggtt tctgctgctg gtgcccagcg agcgtttcgg cttctggttc
    14221 cggttccggc agacgctgac ggcgccccac ttatggctgc tgctctgtat tttgctgctc
    14281 agttatgcgc tggccaggca catcagcaag ccggttcagc aactgcggct ggccgcggag
    14341 aggctgggcc ttggagactt caccgcccgc gtcaactcca gccgccagga tgaactcggc
    14401 gagcttgccc ggacctttga caaaatggcc gagcaaatcg agcatgcggt aaccgcgcag
    14461 cggcaattgc tgcaagacgt atctcacgag ttgcggtcgc cccttagccg actggctgtg
    14521 gcggtggagc tggcccggtc gagctccaat ccgagcgaag cgctcgatcg cgtcgagcgg
    14581 gaggccgccc gcctcaacga actggttagc gagctgctcg ccatgacgcg cgaggagctg
    14641 cggccgacgg aggtgaatct ggctgggctg ctcgaggagg tggtcgaaga tgtgcaaatc
    14701 gaggcgcagg cgcggcaatg ccgcgtagtc ctggacggcg cgcccgcgct gattgtatcg
    14761 gccgactcca ggctgctcca gcgggcggtg gagaacgtaa cgcgcaatgc cgtttactac
    14821 acagctccgg gaaccgatgt tcacgtggcg gcccaacggc gcaatggggg cgtggaaatc
    14881 accgtgcaag acgctggccc aggggtgccc gaagcggccc tgacccgcat cttcgacgcg
    14941 ttctaccgag tggatcgcga ccgcgatcgt tcgactggcg gcgttggcct gggactttcc
    15001 atcgcccgcc gggcgatcga aatgcacggc ggcacaatca cggcctcgaa tacccatccc
    15061 ggattgcgcg tcgtgctgcg gctgcctgac tcccgggtta tttccgaacc cgctcccagc
    15121 gttatgatgg aggagacgtg agcggggccg aaggcacaga ggaaaagcca gtcgcggtcg
    15181 tgctgctgag cggcgggatg gatagctgcg tgaccgccgc ggttgctgcg cgagagcatc
    15241 ggctggccct attgcattcg agctacggcc agaggaccga ggccagggaa aagcaagctt
    15301 tccagcagat tgccgactat taccgcgccg ccttccgcct ggccatcgag cagcactaca
    15361 tcggcagcat cggcggctcg gcgctcactg atccctcgtt gccaattcct gaaggggaac
    15421 tgcgtccgcc ccagagcggg ccgattccca tcacttatgt cccgtttcgc aacgcccatt
    15481 tcctcgcagc cgccgtttct tgggctgaag ttctgggcgc gcgccgcatt ttcatcggag
    15541 cggtcgagca ggatagctcg ggatatccgg attgccggcc ggaatattac cgggccttca
    15601 atgaggtaat ccggctcgga gtgaagccgg aaacacacat cgagatctgc gcaccgctga
    15661 tacacatgaa aaaggccgac ataatccgtc tcggcctcga gctcggcgcg ccgctgagcc
    15721 ttacgtggtc ctgctatcag tcaagcgaag ccgcctgcgg cgtttgtgat agctgtgcgc
    15781 tgcgccttcg cgccttcgct caggcaggcg tgcccgacgc gattccctac cgcaggacgt
    15841 gaaatacctg ctgctggtct gtctcgctgc gagctgcgcc gccggcgaag acctttcctt
    15901 ttggattcag ccgtgttccg ggcaggtggc cgaagactca cgctgtcagg ccgccgatgc
    15961 cgaactggcg gaatgggccc tgcatgcctg gcaagccgcc gcgggcggcg gaatccggtt
    16021 cgagaaggcc gcggaagaga aaagggcgag gatccggatt tattgggctt ccggccgcgc
    16081 gggattgtat ggcgaagcga tgcccatccg ggtcgatggg aagcccggcg cggctgtata
    16141 cgtcttgccc gacgtttccc agcttgggcc cgcgatcgcc gccgccggcc gcgaggatgc
    16201 tctgttccgg caggcggtcg tctacctgac ctgcctgcat gaaactggcc atgccatcgg
    16261 gttgccccac actgccgact acaacgacat catgtacagt ttcgcctatg gaggcgacat
    16321 tctcgagtac ttttcccgat accggaggaa gatatcggtc cgggaacaca ttcggcgaaa
    16381 ctccggcatt tcagcgtcag accaggcgcg gctacggaag atctattccg acggccgata
    16441 ggagcgctat gccttccctg agtcagccct tgcgtccgtg cggccgcgct cgctggaatt
    16501 cttatataat tcgcggagag gcgtcacgct agtcatgcca gaacccatcg atttcccact
    16561 gccgcctgat ctgccccggc cgctcgatga cggcgcggcc gaccaccttc cgggcgcgcc
    16621 gatgccctcc atctctctcg cttccaccgc gggcagaatg gtggatcttt ccggtttgac
    16681 cgcgtccaga accgtgatct actgctatcc gatgaccgga gttccaggga agcccttgcc
    16741 tgaagggtgg gatctgattg ctggggcgcg cggttgcact ccccaaacgt gcggctttcg
    16801 tgatcactat caggacctct tgcagcttcg cgccgatgtg tttggcctga gcacgcaaac
    16861 cacccaatac cagggcgaga tggccggccg cctccgcgtc ccattcgaaa tcttgagcga
    16921 cgctcaattt caactctgtg acgccctgcg tctgccgact ttcgaggtgg acggcatgcg
    16981 gctcctcagg cgcctcacga tgatcatttc cggcgggcga atcgagcatg tcttctatcc
    17041 tgtctttccg ccggatgaga acgccgctca agtcattcgc tggctcaagg accatcccac
    17101 tgcggcatga tcgcccgccg gctttcgcac cgttcttgaa aggcgtttag gcgcgagcgc
    17161 acggggaatg cggtcctgtg ccctgctgtg tctcggtatc ggtatttgcc ttccctgggc
    17221 ttatttctct cgggtcgccc ggatcagggc ttccatgtga tcgaggtatc tctccagggc
    17281 gcgccgcccg gcggaagtga gccggtactc ggtcttggga agacgccctg caaaatattt
    17341 ggcgcaggta atatagcgcg cttcttccag cttgcgggca tgcacgctca gattgccgtc
    17401 ggtggttttc agcagtctct tcaagtcgtt gaagctcagg gcgtcgttga ccgccagggc
    17461 gctcacgata cccagccgca tccgctcgtg aatcagccga tcaagctcca ggggacggcc
    17521 ttctccggct ttgccgcggg cggggcggcc atccggcggc tcggcgcggc tcttatgacg
    17581 atcctcggca agtgcccccg ctctagccac cgtacctcct cgcaatgatg atgccgaaaa
    17641 taatatggaa accgccaaat ccggcggcca tgagccagtt gccccaagcg gccgggcaga
    17701 atagtccgat ggcgccctcg agcatgaagc agaggcccat tacagggacg atgggtacgg
    17761 aaaaggcgcc tcccgtgacg actcccgttc cgtacagcag cagccacatg gcggggatga
    17821 cgccagccag gcccgcccga taaagcacaa tggtaagtaa agccgccacc acaatcggcg
    17881 ggcacaggct gagagcgaat tttcgcgccg ggcctcgaag caccggcatc ttcgccgccc
    17941 gagccttgcg caccatcgcc agagcgccaa ttagaaacgc aagggtcgcc tcgcctagcc
    18001 aggttgcaag ccaagtcacg gcgcccggtt cgcggaccgc gatggccgcc gccagcaggg
    18061 cggtaacgcc catgaccacg ccgcccaggc ccggaacggc agtgaaggat gccgccgatt
    18121 ccatagtctc ccggatgtag cgcagattgt ccatcgcccg cacttcgagg gcgatcggct
    18181 cgggacgagc tttgggaaaa ggacgtgcag atcccatggc ttgaattata cacgcagaga
    18241 tcgctgcgcg gcctcagcgt ttgctgtatc atttggcgtt aatcggagcc gccttatggg
    18301 aatgctctct tgcaaaaatc gaagcgtctg gcgcgcctgg ctgcgcaaga atggcggcaa
    18361 gcgcgacgaa atctggctcc tttactacaa gaacaaatcg gggcagccca gcatcggata
    18421 cgaggatgcg gtggaagaag ccctctgctt tggctggatc gacggaaaga tcaagcgcat
    18481 cgacgaagcg tcttatgcgc aaaggttcac cccgcgaaga cccggcagca gatggtcggc
    18541 catcaacatc cggcgcgcgg agcgcatgat cgcccagggc cggatgacgg cggccgggcg
    18601 gaaggccttc cgttccgggc ggcgccgaga gtcgccgcgc ctgccgacgc gattgccgca
    18661 gccgctccag aaccgctttg agttgcagaa ggacgcctgg gaaaacttcc agcggtttcc
    18721 gccgtattac cggcgcctga ccatcggatg gatagccagc gccaaaaagg aagagacgca
    18781 gctcaaacgg ctggcaaaac tgattgatta ctctgcgcgc caccagcgca tcaagttcat
    18841 gtagccgtgg cgccgggagc gctccgaacc ggctccggcc ggtcaagggg cggcgcctgc
    18901 tctaaccgca tgttatggct cggccggctt tgggtacgcg gcggccgggg ttgccggatt
    18961 caccttgcgc gcctgcgctt ccgcataggc cttctggaca ggttccggaa accccttcag
    19021 catctccgca agtttcactt gaagctgcgc aatgatctgc ggattctccg gcgcgacgtc
    19081 atagctctca tccggatcgc tggaaagatc gtacagctcg gggcgagaca acaaataact
    19141 atggcgcccg ccgggcggcg cgggcgtata aggagccgtg ttgtgccgcg caatatgcag
    19201 cttccagttc atccagcgcg cgcattgcag gtcccagcca tcaaagtaca gcagcggagg
    19261 ccgctcgatt gacttttttc ggcctgtcag gagcggccat atgtcgatgc catcgagcgg
    19321 tttgggcggg aggattccgc cgcacagccg cgtgacggtt ggaaagacgt ccatcatgga
    19381 agccagtgca tcgcagacgt gtccagccgg aatctggccc ggccaccggg cgatgaaagg
    19441 ttctctgacg ccgccttcat aggccgtgtt tttgcggcct cttagtttgc caggacttcc
    19501 ctgataccag gggccgttat cgctggagaa catcaccagc gtgtccttct ctaacccttt
    19561 ctgcctcagg ctttgcagca cttcaccgac gctccagtct agttcctcga caacatcacc
    19621 gtagaggcct tcttgcgact tgccgcggaa ccgcggcgac gccgccagcg ggatgtgcgg
    19681 aaacgtgtgc ggcatataaa gaaagaacgg cgatcgattg gactgcgcta tgaatctctc
    19741 tgcccgttcg gtgtatcgcg cggtaagtga tgcgagatcg gcggtctgct caatgacctc
    19801 agtgttatgc atcaacaccc ggggattcat gtcgttgcta tacggtatgc cgaagtattc
    19861 gtcgaatccg cggctggtgg gcaggaactg cggcggacgg cccaggtgcc atttcccgat
    19921 gcacatagtc ttgtatccgc gagccttcag cacgtccgcc aaggttgtct catccaggtt
    19981 cagaccgcct gtgtcttgtg gaaacaatac acgcgggacg ccgactctcg tgggatagcg
    20041 gccggtgagc agggccgcgc gcgacggaga acacacggga tcggccgaac agaaatttgt
    20101 aaatcggacg ccctcccgag ccaggcgatc cacattgggc gtccgaattt tggagccata
    20161 gcaccccaga tcgccgtagc ctagatcatc gcaaaggata aagacaatat tcggagtctt
    20221 cgcgggagcg gcgatcagcc gcgatccaac ccctgcgctt gccgaaacca gaaacttacg
    20281 ccgggtcata accgtgacgg gggccgatct gcgcaggcgc cctgcccttt tccggccatc
    20341 ttcgtgttgc gtcttcttgt ggcaggccgt ggtgcgcccg gcggaatggc gttcacacct
    20401 tccgcttcct ccgctccgcc agattgaact tctgatacca ggtggattcc gcgccgccgc
    20461 cataacgcga aattatgaca tccagcagat agggtttccc gtcgcgggtg gcggcgatgc
    20521 cgcgcttcag cgccggctcc agatcccgcg ctgtagccac tctttcaccg gcgaccccct
    20581 gactctgggc aagcttcacg aaatcgatgt ccgggtcgcc caaatacatg ccgctataat
    20641 ggcccgtgct ggccatcttt cctttgtagt tataataggc aaaccgcacc gtctggtaat
    20701 tatgattgtt ccagaccacc gtgagaacgg gcgcgccgta gcgcgcctga gtccaaaatc
    20761 cggaagcgct atacataacc gagccatcgc cgatggagca cacgacctga cggtcgggcg
    20821 cgcccagctt ggcgccggta gccgcgccga tgccccagcc gagcgagttg ccggtattag
    20881 ccagtctcat ctgctcattg ggccggaacc cgaagaggaa cgaatcgtat ttgccggtca
    20941 ggttttcggt aaccacgatc gcgtcgcggt cgatggtgcg ggccaggatg gcgcccattt
    21001 catcgggatg catcgggttt cggccgaagt tgccgcgaat ttcggcttcc gttctggcgc
    21061 gcatggccgc agtgagggcg cgcacctgcc cggagcgtga cgcagcggcc gccgccattc
    21121 gttcccgggt cagcatactt cccatcgcct cccgcaggtc gttcagagat tccttcacgt
    21181 cggaaacgag cgccagatca gtggcgtaat tgcggctcat gtcggaagtg ttcacgccga
    21241 cgcggatgat ccgggcctcc agtggaacct cgggcgagtg cggcgtaacc cggccgccga
    21301 aatcccgagc gccgatgacc aaaagcaaat cggcgttcct catgtactca gacgacatac
    21361 tgaaaccgcc gaggtaaaga ggatgcataa cggggaaatt cgcatatccc tggacgccgg
    21421 ccgccacggg gagccccagc ttctcggcaa agtcgagcaa attagcctgc gcccccgact
    21481 tccagacctc atcgcccaca acaaccaggg ggcggcgggc ctcaatgagc atccgcgccg
    21541 catcctgcac ggctccggcg cttgccctga tacgcccgcg gatcatgaag cgctcggccg
    21601 gcaagatttg cgcctttacg cccttggctt ccagagcgta atgggccata gcaagataga
    21661 ccgggccgcc cggctctgtc gcagcgactt tgaaagcgcg gcgcgtcatc aaggccagcg
    21721 cttccgggtt gcgcgcctcc caggagatct tggtgaactg ccggtttact tcct
//
LOCUS       DASREK010000154         6795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2385035, whole genome shotgun sequence.
ACCESSION   DASREK010000154 DASREK010000000
VERSION     DASREK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2385035"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1871
                     /locus_tag="VFA54_07755"
     CDS             186..1871
                     /locus_tag="VFA54_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HZS50738.1"
                     /translation="MKKAIQITGGAVVIYIVLMLITGLAAKALLSSGRIQNVLASLGT
                     NSPVQISASGGDFDLGQWFLFRPSVAVRDLSISNPPGFSQQPLLAARSVSAQIALPSL
                     LHGRTDIRRIVLQEPQLSVETGPNGRTNLEILLGSLRERNGSAAGAPASNKSAPTVFS
                     IAAFSLQSGAIRFMNQSQKQPALTIRDLDVVLTDFALDASCNIRAGARLFGGKTSRFD
                     FTGRAGPFREDSTPAQGVLSVKLAPAEAPAALRVAYFGDLMRDPGERSRMTLEATMQG
                     DLMRTLEGQGKLALENFEVGKDRASRIPLRGTAPLRLAVERALSNPAFALEAQGAVLQ
                     LGDGRWKGGAAARFDGSRLSGSSKGAITGVQIRQMLAAFTSMGDKVSGMAEIPNYQIS
                     FAGRNAEEIRDSLNGAGNITLEKGRFALFDLLNTVEQVVKRASSGDQPASGSTDFTTF
                     QSSFRIANRQVNLPDLVLENGASRIAGRGYFTFLGEISFDLATNVTGGLAASLGGRPD
                     SSGRAQFQVPVKVRGSLESPKVYPDAGRLAVQTATEKAAGLLDRFLNKKKPPQ"
     gene            2114..4858
                     /locus_tag="VFA54_07760"
     CDS             2114..4858
                     /locus_tag="VFA54_07760"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HZS50739.1"
                     /translation="MNPLKQLEKEGQSVWLDYIRRNLITGGELSRLVVEDGLKGVTSN
                     PTIFEKAIAGSSDYDQPLRELLAKNPGADTRALFESIAIEDIRMAADVLRPVYDQTGG
                     ADGFVSIEVAPQLAHDTAATIAEARRLWSSVGRPNLMVKVPATKEGIPAIEALLAEGV
                     NINITLMFSLAHYEAVAGAYISGLARSPEPQKVASVASFFVSRVDTAVDKALAAIGTP
                     EALALQGKIAVANSKKTYQRFREIFYGKDFAALRERGARVQRPLWASTSTKNPAYRDV
                     LYVEELIGKDTVNTMPLPTLQAFRDHGRVRPSLEEGLEDAGAALQRLRALDIDLNAIA
                     EKLQEEGVAAFSTSFDQLLAALEKKRQALRADRAGRQSLRLGAFQNRADARLKAWQSA
                     GFASRLWRKDATLWTAQPQPELTDRLGWLTLPEAMREHAEECAAFAGQVRSDGVRHVV
                     VLGMGGSSLAPDVFGRTFGHADGFPELIVLDSTHPAAVRAVEKQIDLRSTLFVVSSKS
                     GATIEPLSFFRYFWSLVGRVTKTPGDRFAAITDPNTPLERLAQQRGFRRIFLAPPDIG
                     GRYSALSVFGLVPAALIGLDVHHLLDRAWTMAEACASCVPVPENPALALGAAMGELAL
                     TGRDKVTFLAPPALASFPSWAEQLIAESTGKDGKGIIPVAEEPLGKVDSYGSDRFFVY
                     LGLNGAADSALEEDLERVEKAGHPTARIQLAETADLGQEFFRWEMAVAAAGAVLGIHP
                     FNQPDVELAKELARQEMKRGGVSGGGAAAPAPQSGAMRAALDQLLSQARPGDYVAIQA
                     YLPPDPATTKRLQVIRVQLRDRLRLATTLGYGPRFLHSTGQLHKGGPNTGLFLQLIDD
                     PEEALPVPETDYSFAALIRAQAFGDYQALQQRGRRIVQIHLGRDVTGRLQELEEAANG
                     "
     gene            4851..5474
                     /locus_tag="VFA54_07765"
     CDS             4851..5474
                     /locus_tag="VFA54_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HZS50740.1"
                     /translation="MGDVAIAPRALFWDVGGVLLSNAWDRGIRRRAVAHFQLDWEEFE
                     DRHELVVPAFEKGQLTLDEYLDRTVFYRARLFTKESFREWICAQSTPQPESLALVERL
                     ARTKKYVMGTLNNESAELNEYRIRRFHLRNYFAVFLSSCYLGVRKPDAAMYRLAMQLA
                     QCEPHECIFVDDRPLNVECARRAGMHAIQFENAGRLEPALRQLGVDW"
     gene            5911..>6795
                     /locus_tag="VFA54_07770"
     CDS             5911..>6795
                     /locus_tag="VFA54_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HZS50741.1"
                     /translation="MPSPRHILTINSGSSSIKFALYGMAESESLVLSGSLQRIGLDSG
                     FFRARGSRGEALIEESDDLPDHEAALQRLFAWLPQHSPADLAAVGHRLVYGGLEYTQP
                     RWITRDLTTALQKLIAFAPEHLPHELKAIEAVSRAHPDLKQAACFDTAFHSQMPKIAR
                     LYPLPRFLWDEGVRRYGFHGLSYSYIMGELAEQAGLEAANGRVIIAHLGNGASMAAVR
                     GGRPIDTTMGFTPAGGLMMGTRSGDLDPGVMLYLLEQKGLSPVRTRDIVNRQAGILGV
                     SGISSDMQDLLAKEADDPH"
BASE COUNT         1299 a         2078 c         2132 g         1286 t
ORIGIN      
        1 aaaatgcaaa tgacagaggc acattcgtcg ctgcgttcac aatgactggc gaattcttcg
       61 ccaggctccg gcgtcgaaga cctttactgc tacaatcacg gaagcacagg ccagcgaggc
      121 aagccggacg gctgcggccg ccgggacggc gcctgatccg tcacgttctg ctggaagggg
      181 gcggtatgaa gaaagcgatt cagattaccg gcggcgccgt tgttatctat atcgttctga
      241 tgctcatcac ggggttggca gcgaaggcgc tgctctcttc cggccgtata cagaacgtgc
      301 tcgcgtcgct cggaaccaat tcgcccgttc agatatccgc tagcggaggc gacttcgatc
      361 tggggcagtg gtttcttttc cggccgtcgg tcgccgtccg ggatttgtcc atcagcaacc
      421 ctccgggctt ttcacaacag ccgttgcttg ccgccaggtc cgtctcggcc cagatcgccc
      481 tgccgtccct gctgcacgga cgcaccgata tccggcggat cgtgttacaa gagccgcagc
      541 tcagcgtcga gaccggcccc aacggccgta ccaacctgga aattctgctc gggtccttga
      601 gggagcggaa tggctccgcc gccggcgctc cggcatccaa caaatcagcg ccgactgtct
      661 tttctatcgc cgcgttttct ttgcaatccg gagcgattcg attcatgaac cagtcgcaaa
      721 agcagccggc gcttaccatc cgtgatttgg acgtcgtcct gacggatttt gcactggacg
      781 cgagctgcaa cattcgagcg ggcgcgcgcc ttttcggcgg caaaacgtcc cgtttcgatt
      841 tcaccggacg cgccggacct ttccgggagg attcgacgcc cgcgcaaggc gtcctctccg
      901 tgaagctggc gccggccgag gcgcccgccg cccttcgcgt tgcatacttc ggcgacctga
      961 tgcgcgatcc cggggaaaga agccgcatga ccttggaagc cacgatgcaa ggcgatttga
     1021 tgcggacttt ggagggccag ggaaaactcg ctctggaaaa ctttgaggtg ggcaaagatc
     1081 gagccagccg gattccattg cggggaaccg ctcccctgcg cctggcagta gagcgcgcac
     1141 tgagcaaccc tgccttcgct ttggaagcac aaggcgctgt gctgcaactg ggcgatgggc
     1201 ggtggaaagg cggggctgcg gcgcggtttg acggttcgcg cctctcgggc agcagcaagg
     1261 gcgccatcac cggagtgcag atccgtcaga tgctggccgc atttacttcg atgggagata
     1321 aagtgtctgg catggctgaa attcccaatt accaaatcag cttcgccggc cggaacgccg
     1381 aagaaatccg cgactcgctc aacggcgcag gaaacattac gctggaaaag ggccgcttcg
     1441 cccttttcga tctgctcaac accgtcgagc aggtggtcaa aagggcttcc agcggcgacc
     1501 agccggcctc aggttctacg gacttcacca ctttccagag cagctttcga atcgccaacc
     1561 ggcaggtgaa cctgccggat ttggtcctcg aaaatggcgc ctcccgcatt gcaggccggg
     1621 gctatttcac atttttgggc gaaatcagct tcgatttggc caccaacgtc accggcgggc
     1681 tggcggcctc gctgggcggg cgccccgaca gttccggacg ggcgcagttc caggtgccgg
     1741 tcaaggtgcg gggttcgctg gagtcgccga aggtctaccc cgatgcgggg cggctcgccg
     1801 tacaaacggc cacagaaaag gcggcaggac tgctcgaccg cttccttaac aaaaagaagc
     1861 cgccgcaata aagcaagggc gttcaccaga ggtgcggcga gacagccccg cccgcggttc
     1921 gacgcgctct ctccggccgt gtggctaaat cctaacagct cgcctgcggg gattcctgca
     1981 ataggaggaa gggcccgggc ggtttaagtg aaatctgcaa cgagcgcacg ggcgcaggac
     2041 tccaatcgct gtactccgag cagttctgtt gtttctacgg aagctgttat tttcgaaccg
     2101 gaggttgccc gcgatgaacc ctctcaaaca actcgagaag gaaggccagt ccgtctggct
     2161 cgactacatt cggcgcaatc tgatcacggg cggcgagctc tcgcggctcg ttgtggaaga
     2221 cggcctgaaa ggagtgacca gcaatccgac gatcttcgag aaagcgattg cgggcagctc
     2281 cgattatgac cagccgctgc gggaactgtt ggccaagaac cctggggccg atacccgggc
     2341 gctgttcgag tcgattgcca tcgaagacat ccgcatggcg gccgacgtgc tccggccggt
     2401 ttacgatcag accggcggcg ccgatggctt tgtgagcatc gaggtcgcgc cgcaactcgc
     2461 gcacgacaca gcggccacta tcgccgaggc gcgccgtttg tggtccagcg taggccgtcc
     2521 caacctgatg gtgaaagtgc cggcgacgaa ggaaggcatt ccggcgattg aagcgcttct
     2581 tgccgagggc gtgaacatca atatcacgct gatgttttct ctggctcatt atgaggctgt
     2641 agcgggggcc tatatcagcg gcctggcgcg ctcgcccgag ccgcagaagg tagcgtcggt
     2701 ggcttctttt ttcgtgagcc gcgtggacac ggcggtcgac aaggccctgg cggccatcgg
     2761 gacgcccgaa gccctggcct tgcaaggcaa gatcgccgtc gccaactcga agaagactta
     2821 tcagcgcttc cgtgaaattt tctacggaaa agatttcgcc gcgctgcgcg agcggggcgc
     2881 ccgcgttcag aggccgctgt gggccagcac cagcacgaaa aacccggcat atcgcgatgt
     2941 gctctacgtg gaggagctga tcggcaaaga tactgtcaac accatgccgc ttcccacgct
     3001 gcaagccttc cgcgatcatg ggcgcgtgcg gccgtcgctc gaagaagggc tggaggacgc
     3061 cggcgccgcc ctccagcggc tgagggctct cgatattgat ttgaacgcga tcgcggaaaa
     3121 gctccaggag gaaggcgtag cggcgttctc aacctccttc gatcagttgc tggccgcact
     3181 cgagaagaag cggcaagcct tgagggctga ccgggccggc cggcaaagtc ttcgtcttgg
     3241 ggccttccag aaccgcgccg acgcccggtt aaaagcctgg caatccgccg ggttcgcatc
     3301 ccggctttgg cggaaagacg ccacgctttg gaccgcccag ccgcagcctg aattgacgga
     3361 ccgcctcggc tggctcactc ttcccgaggc gatgcgcgag cacgccgagg agtgcgccgc
     3421 cttcgccggg caggtgaggt ccgacggcgt gcgtcatgtc gtagtacttg gcatgggggg
     3481 atccagcctg gcgcccgatg tattcggccg aaccttcggc cacgcggacg gctttccgga
     3541 gctgatcgtg cttgacagca cccatcccgc ggccgtgcgg gcggtcgaga agcagatcga
     3601 tttgcggagc actctgttcg tggtttcgag caaatcgggc gccaccatcg agccgctttc
     3661 tttcttccgg tatttctgga gcctggtggg ccgcgtgacg aagacgccgg gagatcgttt
     3721 cgccgccatc acggatccca acacgccgct cgagcgcctg gcgcagcagc gcgggttccg
     3781 gcgcatcttc ctcgcgccgc cggatatcgg cggccgctat tccgccttga gcgtctttgg
     3841 cctggttccg gcggctctga ttggactcga cgtgcatcac ctgctggacc gcgcctggac
     3901 tatggcggaa gcgtgcgcct cctgcgtgcc cgtccctgaa aatccggcgc ttgcactggg
     3961 cgcggcgatg ggggaactgg cgctcactgg cagagataag gtcacctttc ttgccccgcc
     4021 ggccctagca tcgtttcctt cctgggccga gcaattgatc gcggaaagca caggcaagga
     4081 cggcaaaggc atcattccag tagccgagga gccgctcggc aaggttgact cttacggctc
     4141 cgaccgcttt tttgtgtatc ttggtctgaa cggcgcggcg gattccgcgc tggaagagga
     4201 cctcgagcgg gtggagaagg ccgggcatcc gacggcgcgc attcaactgg ccgagacggc
     4261 tgatctcggc caggaattct tccgctggga gatggctgtg gcggctgccg gcgccgtgct
     4321 cggaattcat cccttcaacc agccggatgt cgagctggcc aaggagctgg ctcgccagga
     4381 gatgaagaga ggaggcgtct ccggcggggg agccgccgcg ccggcgcccc agagcggagc
     4441 catgcgcgcg gccctggatc aactgctctc gcaagccagg ccaggcgact atgtggccat
     4501 ccaggcctac ctcccgccgg atcccgccac caccaaacgc cttcaggtga ttcgagtgca
     4561 attgcgcgac cggctgcgtc tcgcgacaac cctgggctat gggccgcgct ttttgcactc
     4621 cacgggacag ctccacaagg gcggtcccaa caccggtctc tttttgcaat tgatcgacga
     4681 tcccgaggag gctctacccg ttccggaaac cgactattcc tttgcggcgc tgatccgggc
     4741 gcaggccttc ggcgattatc aggctttgca acagcgcggc cggcgcatcg tgcaaatcca
     4801 tctgggacga gatgtgacag gcaggctgca agagctggag gaggccgcca atgggtgatg
     4861 tcgcgatcgc accgcgcgcc ctgttctggg acgtgggcgg cgtgctgctt tcgaacgcgt
     4921 gggaccgcgg catccgccgg cgcgccgtcg cgcactttca gctcgactgg gaagaatttg
     4981 aggaccgcca cgagctcgtc gtgcctgcat tcgaaaaagg gcaactgacg ctcgacgaat
     5041 acctggatcg aaccgttttc taccgggccc ggctgttcac gaaggagtct ttccgggagt
     5101 ggatctgcgc gcagtccact ccccagccgg aaagcctggc ccttgtggag cgcctggccc
     5161 gcacgaagaa atatgtgatg gggacgctca acaatgaatc ggcggagttg aatgaatacc
     5221 gcatccggcg ttttcatttg cgaaactatt ttgccgtttt tctgagctcc tgctatctcg
     5281 gtgtgagaaa gcccgatgcg gccatgtacc gtctggctat gcagctcgcg cagtgcgagc
     5341 cgcacgaatg catatttgtg gatgacaggc cgctgaacgt cgagtgcgcc cggagagccg
     5401 gaatgcacgc gattcagttt gaaaacgcag ggcgcctcga gccagcgctg cggcaattgg
     5461 gtgtggattg gtgaaaaccc tgacgatcaa accctctttc ggaaaacgga cggttttggg
     5521 aaatacgttc gaattgaaaa caaatcccat aatttatatc ggccggaacc agcgatacca
     5581 gcgcatcata tccgaaatat tggacggaga attatgcaaa gggtgcagga aacgggccta
     5641 tcgctggaat gaatttttgt ccgacgaccg aagaggggtt gaaatggctc ggcatcccta
     5701 tcggaccagt tctctgagca aatggccata ggtagggccg gctgaaaccg cgacctattc
     5761 ggccgcattg agttcttggc aaagcgatcg tactgaagct ccatggcgct gcgccgaggg
     5821 gccacagcta tagaaggcgg gtaatatagg aaaatcagtc tgggctttcg cccggccgtc
     5881 tggcagatgg ctgcggcccc ctcacggaag atgccgtccc cgcgccacat cctgaccatc
     5941 aacagcggct cgtccagcat caagttcgcg ctatacggca tggccgaaag cgagagcctc
     6001 gttctctcgg gcagcttgca gaggatcggc ctggacagcg gattttttcg cgcgcgcggc
     6061 tcgcgcgggg aagccctgat cgaggaatcg gacgatctcc cggaccatga ggcagcgttg
     6121 caacggctgt tcgcctggct tccccaacac tcgcccgcgg atctggcggc ggtaggccac
     6181 cgcctggtct acggcggcct ggagtacacg cagccccgct ggatcactcg ggacttgaca
     6241 acggcgctcc agaagctgat cgccttcgcc cccgagcatc tccctcatga actgaaagcg
     6301 atcgaggccg tcagccgcgc gcaccccgat ctgaagcagg cggcttgctt cgacactgct
     6361 tttcactcgc aaatgccgaa gattgcgcgg ctgtatcctc ttccccgttt tctgtgggac
     6421 gagggtgtcc ggcgatacgg ttttcacgga ttatcttact catacataat gggagagctg
     6481 gcggaacagg ctggcctgga ggccgcaaac gggcgcgtga tcatcgcgca cctgggcaac
     6541 ggggccagca tggccgcggt tcggggaggg cggcctatcg acacgaccat gggcttcacg
     6601 cctgccgggg gactcatgat gggtacgcga tccggcgacc tcgatccggg cgtcatgctc
     6661 tacctgctcg agcagaaggg cctttcccca gtccgcacga gagatatcgt caaccgccaa
     6721 gccggaatcc tgggcgtttc cggcatcagc tccgatatgc aagacctctt agcaaaagaa
     6781 gccgacgatc cccat
//
LOCUS       DASREK010000155        16538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1401002, whole genome shotgun sequence.
ACCESSION   DASREK010000155 DASREK010000000
VERSION     DASREK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 16538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16538
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401002"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..2368)
                     /locus_tag="VFA54_07775"
     CDS             complement(32..2368)
                     /locus_tag="VFA54_07775"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HZS50742.1"
                     /translation="MPPREITFRLREKITSEMERIGVGGARFHPSDFREFKSRLAGAL
                     TSRFYCRRPKEAAKFIRQHRPEWAGRAVEEAEQLCRHIVELLGYGPLELGTEINWHRD
                     PLTGRVWERRFWRDYNLPQDCSAGDPKTIHELNRHQYLPRLAKAYLLTGEERYAAEAL
                     QQIESWIEQNPPGMGIHWHSSLEIGIRAISWMWTLFLLIDSLSLEEAPALRIGASLFA
                     QLEHVYRHTSVFSSPNTHLIGEAAALFIAGLVFQNSFRPAAAWLDRGAELLAMEAGKQ
                     VLSDGVYGELSSYYHCYALDFYLQALILADQNAFRFPENVRRTAGSMLDFLLHVTRPD
                     GSLPLIGDDDGGRALALVQKNYRSFRDALCLGSVLFLREDFKHQAGEFAEEALWLLGP
                     EAHEIYSLLRAKTPAANQAFYPRAGYWIARSGWDPPASHLIFDCGGLGMGAGGHAHAD
                     ALSLSLFASGRELLVDPGTFIYNGAPEWRSYFRSTRAHNTVVIDDQDQAAAADTFRWK
                     TKLPCRVLEHLARPEIQYLDAEHAGYSRDRQGVIHRRRLLQAAPGHWIVVDDFRGCGA
                     HTFDLCYHFGPGAQLSLVRQNGGRELAISAAYGDAALHLCICASGPLYADFICGSSDP
                     IAGWISHQYGQKLPSTAFRARLESAVPAAAVTFLIMPSCQAFSASPPLATRLAVKGEG
                     AIACSCDYQGFEDVVVFSDGETEIQVEAFRMRGEFFWLRMRDGILKRVLAIRAQSLSQ
                     SGRTVFEKQNPGTYFAAKDAGLEKSDVRDLRNRQFRRG"
     gene            complement(2409..3467)
                     /locus_tag="VFA54_07780"
     CDS             complement(2409..3467)
                     /locus_tag="VFA54_07780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS50743.1"
                     /translation="MRLKVLLMGPYPPPYGGVSVHVLAAHKLLTEAGLSCQVLNLPEP
                     SRAKGAARLRHWARLIRSLRRRAKQGWIIHVHINGHNWKSWAVAAIGGVSGSAAAGRI
                     LTLHSGMVAKYLGTASAWRRALAAFACARFTRIVCVTTEIRAALLSLGVPIERTEILP
                     AYLGAVPRKQGIEDWLLSWIREHSPVISTALFFRPEYGFRLLVEAVSRLRSRYPRLGC
                     LVMGSGEEQTEAEQSIEKQGLQNAMLLLGDVDHETCLALISASDVFVRPTFEDGDSVS
                     VREALALGVPVVASTAGVRPGEAILFQSGNLEDLLSSFERALAAPPARHGQAPDGTAR
                     LIEIYRQVATTGAEYAAA"
     gene            complement(3458..4594)
                     /locus_tag="VFA54_07785"
     CDS             complement(3458..4594)
                     /locus_tag="VFA54_07785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS50744.1"
                     /translation="MQLQKIRLLKLVTNFKIGGTERQVANVALRLDSSRFELHLGSLR
                     RHGELLKDLETLDVPQPEFQIGKFYDGKTFLQAARFAQYIRRNLIQVVHSYGFYSNVF
                     AVPAARMAGRCVVVASIRDTGEVMTPMQRRVQKAVCRMADCVLVNAEAIRRTLIEQGY
                     RPETIFVIRNGIMLSRFAAKQNGHLLRLQLGLPASAQLVAVSSRLNRMKGIEYFLDAA
                     AILAAKFPNVAFLVVGDGDIRQELEDHARRVGVGRRVIFTGFRSDVPELLSEVAVSVL
                     PSLSEGLSNSLLESMAAGVPVVATRVGGNPEVIEDGVTGWLVPARDPASLAEAIGRLL
                     EDREQAFRFGQNGRRRVAELFSIERAVQETEKLYSRLVEAKLCA"
     gene            complement(4607..6487)
                     /gene="asnB"
                     /locus_tag="VFA54_07790"
     CDS             complement(4607..6487)
                     /gene="asnB"
                     /locus_tag="VFA54_07790"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZS50745.1"
                     /translation="MCGIAGIFSYGGKPVLGHEVRAMCSAIVHRGPDDEGLYLGSGVG
                     LGMRRLSIIDLDSGRQPVRNEDGSVWVVFNGEIYNFRELVIRLRARGHRFYTRSDTET
                     IVHLYEEYGDRCVDHMRGMFAFALWDEKKRRLLLARDRLGIKPLYYADVGGRLLFGSE
                     IKALLELQEVERRLNWDAVAHCLSYLVTPPNESAIAGVHKLPPAHLMTASADRPPAVS
                     RYWDLHFEPDYGKTEKQFIEGLREHLEESVCLHLISDVPLGAFLSGGIDSSSVVASAA
                     RQKTEPLKTFSIGFAEQEFNELGAARRVAEAFHTDHHELVLQPDILERLDHLAWHLDE
                     PFGDSSAIATYMVSQMAAQHVKVVLSGDGGDELFAGYDKYVVEQRERSRAPLPAPARK
                     MLAFISRAMPEGMRGRNFLRHASRTGFERYLDASTFFRREELRRLFEPEIFAMLSPEP
                     DPGRGAEPDGLEGRHWLTRLQYLDIQRYLPLDILTKVDRMSMAHSIETRVPLLDHKLV
                     EFAATIPPEMQMRNGTTKYILKRAMRGILPDEIINRPKHGFAVPLRYWFRNRLSNYAR
                     ELLLSAPSRRRGVFNQQYVEKILHLHEKGRDFDLQLWTLISFELWSRAILDRGARTEV
                     AA"
     gene            complement(6499..8028)
                     /locus_tag="VFA54_07795"
     CDS             complement(6499..8028)
                     /locus_tag="VFA54_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50746.1"
                     /translation="MTLAADRQIRSTDSIFRPALLLMSGRIMAFAATSFIPVILAGIF
                     DRAEFGTYKQLFLIYTTVFLIAQIGMSSSLYFFLPRFASEAGRFVANSALCLAASGLA
                     CLGFLALGGPWLARWFSNPGLEPYMIWIGLFLSLMMVSSLLEIVMVSRGRYLWASVSY
                     ALSDLTRAAALIIPALIVARLDAVLKAAVGVAALRAGAMMWYLRREFGGGLRPDAGLL
                     KRQLLYALPFGMAVVVEIAQSNLHQYAVSHRFDPATFAVYAVGCVQIPFVDFVANPTS
                     DVMMVKMQERLAEGRQEAVLRIWHETTRKLALLFFPLAGFLAAAAKEMIVFAFPKYAA
                     SAPVFAVWSAGVMLAVFQIDGVLRVYAETRFLLAMNLMRLAIIASLVGWFLSEFYLLG
                     AVLVTMLAALAGKAAALIRIKKLLCADFAGVLPWRNLAAVWCAAIAAATIAMAAKPHL
                     PLATGRNLPTFTALAATAGIYGAAYAAMLWHFGLLRKEEKLAVTGWFRQAVGAAARAF
                     S"
     gene            complement(8025..9287)
                     /locus_tag="VFA54_07800"
     CDS             complement(8025..9287)
                     /locus_tag="VFA54_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS50747.1"
                     /translation="MPKYNQPELAMEEGGGTAIQFARRAVPATSISYWLLLIFVFLLY
                     TSLPLQYPALEAVRPAQLTAIAALIILSMEVVFAGRSFDPAWPQGYALLGFLGAAALS
                     CSTALWPRLAFESTLDFLKIALIFFVVVNCVDSERRVRGLMWVMLIGGLAPAAGALRY
                     YAAGQTVEGRAHWLGIFANPNELAASLVVLLPLAGCLAAKCRLTGRVALLGFSLLYAA
                     AIFLTFSRGGMVGLLAALLLYAWRRRSAAVLCLLAAAASVALVSAGRYWSRDEGFSDL
                     SQDVSFRQRLATSRAGLAMFADHPLLGVGFGCSLIAWPLYAPEDLYRQGRGELVTHNT
                     FIQPLSETGLAGFIPFILFLGSGLATARKLARRRPAGNITPLAAGLEIALWGFAVCGL
                     SGGYVLSWFPYILTGLICSAKRAAEQNS"
     gene            complement(9274..10377)
                     /locus_tag="VFA54_07805"
     CDS             complement(9274..10377)
                     /locus_tag="VFA54_07805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZS50748.1"
                     /translation="MMIRTLVKTGAAAALARSGMGQLFRVLSRMRNSPVIVAYHRVVE
                     NFASSAETSIPSMLVSRRTLEQHLDSFGRRFRFVSLDELGAELEGRARCGKPMAAVTF
                     DDGYRDFYDYALPLLRAKGIPAAVFVVTATLGTADLHAHDKLYLLVSKRFGRDKWPPP
                     HFIKLLQSLKIRLPHFKPRDAFEATRVLLENLPRAELDKVLAKLASETEIPEEVRKPF
                     RSLTWEMLAEIHKAGILIGSHSRTHAQMTRESRQRVLDEAQESRRELEKRLGLPIRHF
                     AYPNGQFDSISVDAVASAGYRFAYTICSHRNHVHPLLTVPRTVLWENACRDSHGSFSS
                     AMLNCHLDRVFDLFVERCRQNHTIVRRLLDAEV"
     gene            complement(10374..11564)
                     /locus_tag="VFA54_07810"
     CDS             complement(10374..11564)
                     /locus_tag="VFA54_07810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS50749.1"
                     /translation="MTTTRAETGGECLAVERVSDIHEFERLRTEWDELLEASAVNCLF
                     LTWEWLYTWWKHLRANRKLWILTVRREGKLTGLAPLALRPPSLMRSHPLPVLEFLGSG
                     FAGSDYLDFIIRRGCEPESLRALAGKLAPELLMIELSQLKRGASLAERAIPIFAAKGW
                     RASPSVSNVCPFISLEGHSWESYLEQLGSEHRYNIRRKLRRMEKEFDAQFEEIKTEGP
                     LGPALEEVVRLHHLRWDARGGSDAFHTSELLAFHEEFAQIALSRGWLRLYLLRLDGAP
                     AAGVYGFLYRRVFYFYQSGFDPAYSKNSVGLAAIGLSIKSAIAEGALEYDFLHGGEEY
                     KSRWSRQSRDLLKLELYPPRARGWLYRSSLEIGRASRAVARRVLPQTVVNRLTVKAHR
                     SSRR"
     gene            complement(11591..11728)
                     /locus_tag="VFA54_07815"
     CDS             complement(11591..11728)
                     /locus_tag="VFA54_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50750.1"
                     /translation="MTGIWRKLALLSYTFVLMNWAAVAGLYNFARGRMDVWHSGGTAA
                     R"
     gene            complement(11725..12792)
                     /locus_tag="VFA54_07820"
     CDS             complement(11725..12792)
                     /locus_tag="VFA54_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS50751.1"
                     /translation="MAEAVFWISVIFIAYVYFGYPLLLAAWKLAAPRRVHKSYGEPPV
                     SIVIAMRNERANAERKIRNCMELDYPAEKMQVIVSLDGPTDGVEAVVRPYASRGVEIV
                     FSPVHRGKAAALNAAMEAATGEIVVFTDARQQLHRDAVRELAANFSDPEVGAVSGELV
                     LLEESGREASSAIGVYWRYEKWLRALESATHSVIGATGAIYAIRRELFTPLPPGTILD
                     DVLIPMRIVLGGKRVVFDSSARAYDLAAANPKAEYARKARTLTGNYQLLAEMPALLAP
                     WRNPVFLQFLSHKAGRLLVPYFLVGLFVSNLPLEQGIYRAAFLLQLFWYALAAIGALA
                     ARRRPPGTPAEIECGRNQRQI"
     gene            complement(12805..14640)
                     /gene="glmS"
                     /locus_tag="VFA54_07825"
     CDS             complement(12805..14640)
                     /gene="glmS"
                     /locus_tag="VFA54_07825"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZS50752.1"
                     /translation="MCGIIGYIGTRDAPQVLLEGLKRLEYRGYDSCGIALLDNGSPKV
                     LRSAGRVAMLEEKALALPKSSSRFKCGIGHTRWATHGRPSEINAHPHWDCTGHILVAH
                     NGIIENHISLREELKLEGHVFRTETDTEVLPHLIEAHFNGCLEEAVRAALQQVDGLYS
                     LVVISTRDNMKIVAARRGAPLVVGVGEQESFVASDAPALLPYTRDMLFLDDGEMAIIR
                     PDSMELRTLAGSSVARRPQPIAWNSEMAEKNGFPHFMLKEIFEQPRALRETLCGRLKN
                     GQSVNLDDEISRREVFEGLERMHVVACGTSLHAALVGKFFIENLARLRVEVDYASEFR
                     YRNPLIGRGSMVVAITQSGETADTLAALREAGDRRAPRLAICNVVGSMAARQAENVLY
                     TRAGPEIGVASTKAFTTQLAVLYLLALWLAQARRTLPPEQVRVRVRALEQIPDQMQEI
                     LNRSGSIESLAQQFCSYRSFLYLGRGVNYPLAMEGALKLKEVSYIHAEGYPAGEMKHG
                     PIALIDADMPVAVLAPRDSVYKKTLGNIEEVAARGGKLLAVVTENDGDIAKLTPHTIP
                     VPYTNDLLYPLLAALPLQLFAYHAAVLRNCDVDKPRNLAKSVTVE"
     gene            complement(14654..15676)
                     /locus_tag="VFA54_07830"
     CDS             complement(14654..15676)
                     /locus_tag="VFA54_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HZS50753.1"
                     /translation="MTRLSIVGIGGGTGLPVLLRGLQPFADGSHSRLSVDVKVSAIVC
                     VTDNGGSSGRLRRSFNIPAVGDLRNCLVALSPDDSVLAELFQHRFSDGNGLAGHALGN
                     LIVTALCQKSGSLGRAAALAGQLLPLKGEALPVTEAPVTLCARLHDRTIVRGESEIGA
                     AGGRIEQVWLEPDGVSPAPGVLEAITHADALVLGPGSLYTSLIPNLLVAGVAEAIGRS
                     RALKIFVCNLMTEPGETDGFSAADHLRTLAAYLGPRPADFCIVNSRLAPAARTLTGRR
                     DCCQPVPCDWREIERLGAMPICADLLSPQEEKIRHDPATLGHIVMSLARGALHGFGGF
                     SSSAAA"
     gene            complement(15676..>16538)
                     /locus_tag="VFA54_07835"
     CDS             complement(15676..>16538)
                     /locus_tag="VFA54_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HZS50754.1"
                     /translation="IEFPAAPRGGAAVIDCDQIPELAARERISCVIVAERDSQRRTEV
                     AAALLDCRLRGLRVVDAVDFYEKLSGKIWLEGLHPQWLVYTDGFDRSRLTVCAKRSLD
                     LLFAALLLLLTSPVLAIVAAAIRLDSGGPVLFRQVRVGQHGKPFVLYKFRSMRQDAEA
                     ETGPVWAGEHDSRVTTVGKILRKFRLDEIPQAFNVLRGDMSFVGPRPERPYFVQMLRP
                     RIPFYDLRHCVKPGITGWAQIEYPYGASIEDAYEKLQYDLYYAKHMSLRCDAVILLRT
                     LGIVLFGRGR"
BASE COUNT         3507 a         5212 c         4904 g         2915 t
ORIGIN      
        1 cattctgccg atagtcggct gatccactgg gtcagccgcg tcgaaattga cgattccgca
       61 aatcgcgcac atcgcttttc tccaggccgg cgtctttcgc ggcaaagtaa gttccggggt
      121 tttgcttctc aaacactgtt cggccgcttt ggctgagact ctgggcgcgg atcgccagga
      181 cacgcttcaa aatgccgtct ctcattcgaa gccaaaagaa ctcgccccgc atacggaagg
      241 cttcaacctg aatctcagtt tctccgtctg agaacacgac gacatcctcg aaaccctggt
      301 agtcgcaaga acacgcaatc gcgccctccc ctttcaccgc cagccgtgtg gcgagcggcg
      361 gagaagccga aaaggcctgg caagaaggca ttattagaaa cgtcacagcc gccgcaggca
      421 ctgcgctttc cagccgcgct cgaaaagccg tgctcggaag cttttggccg tattgatgcg
      481 atatccatcc ggcgatcgga tcactgctgc cacagatgaa atccgcatag agcggcccgg
      541 acgcgcagat acagagatga agagctgcgt ctccgtacgc agcggagatg gccaattccc
      601 gtccgccatt ctgccgcacg agtgagagct gtgcgcccgg accgaagtgg tagcacaaat
      661 cgaatgtatg cgcgccacag ccgcgaaaat cgtccacgac gatccaatga ccgggcgccg
      721 cctgaagcag gcgccgccga tggatcacgc cttgccggtc ccggctatac cccgcgtgct
      781 ccgcatccaa gtactgaatc tccggccggg ccagatgctc cagcacccgg cacggcagct
      841 ttgtcttcca ccggaatgtg tctgcggccg ccgcctggtc ctgatcatcg ataactacgg
      901 tgttgtgcgc gcgggtcgag cggaaatagc tgcgccattc cggcgctccg ttatagataa
      961 aggttccggg atcaaccagc aactcccggc cgctcgcgaa cagggaaagg gaaagcgcat
     1021 cggcatgggc gtggcctccc gctcccatac cgaggccgcc gcagtcgaaa atcaagtggc
     1081 tggccggcgg gtcccagccg gagcgcgcaa tccaatagcc ggcgcggggg taaaaggcct
     1141 gattcgccgc cggcgttttg gcccgcagca atgagtagat ttcgtgcgcc tcgggaccca
     1201 gcagccaaag cgcctcctcc gcgaactcac cggcttgatg cttgaaatcc tcgcgcaaga
     1261 aaagtacgct gcccagacat agagcgtcgc ggaacgagcg gtagtttttc tggaccagag
     1321 cgagggcccg gccgccgtcg tcatctccta tcagcggaag cgacccatcc ggacgagtga
     1381 cgtgcagcag aaaatccagc atagagccgg ccgtgcgccg cacgttctcc gggaatcgga
     1441 aggcgttttg gtccgcgaga atcagcgcct ggagatagaa atcgagggca tagcagtggt
     1501 aatacgatga cagttcgccg tagacgccgt cgctcagaac ctgcttgccc gcctccatag
     1561 ccagcaactc cgcgccacga tcgagccagg ctgccgccgg cctgaacgag ttttggaaaa
     1621 ccaagcccgc gatgaatagc gccgcggcct cgccgataag gtgcgtattg gggctgctga
     1681 acacagaagt gtggcggtag acgtgttcca gttgggcgaa cagagaggcc ccgatacgca
     1741 gagccggcgc ctcctcgaga gaaagagaat cgataagcag gaagagagtc cacatccagg
     1801 aaattgcccg aatgccgatt tccaggctcg agtgccaatg gatgcccatg ccgggaggat
     1861 tttgctcgat ccaactctcg atttgctgca acgcttcggc ggcgtagcgc tcctctccgg
     1921 taaggagata agctttcgcc agccgcggca gatactgatg ccggttcagt tcgtgaattg
     1981 tcttggggtc gccggcggag cagtcttgcg ggagattgta gtcgcgccag aagcggcgtt
     2041 cccataccct gccagtgagc ggatcacgat gccaattgat ctccgttccc agctcgaggg
     2101 gaccgtagcc aagcaattcg actatatggc ggcaaagctg ttccgcctcc tcgacagccc
     2161 tgccggccca ctccggacgg tgctggcgta tgaatttggc ggcttccttc gggcggcggc
     2221 aatagaagcg gctggtcaga gcgccggcga ggcggctctt aaattcccgg aaatcggacg
     2281 gatggaatct ggcgccgccg acgccgatcc gctccatttc tgaagttatc ttttcccgca
     2341 ggcggaatgt gatttcccgg ggcggcatag cgcttagccg tttgagcttg tgcgcggccc
     2401 cgctcctttc aagcggcggc atattcggct cctgtagttg cgacttgccg gtagatttcg
     2461 atcaagcgag cggttccatc cggcgcttgc ccgtgcctgg caggcggagc agccagcgcc
     2521 cgctcgaaac tggaaagcaa gtcttccaaa ttccccgatt gaaagaggat ggcttcgccg
     2581 ggccgcacgc cggctgtgct cgcgacgacg ggaacgccga gagccagagc ctcgcgcacc
     2641 gagacggagt cgccgtcctc gaatgtcggc cttacaaata cgtcgctggc cgaaatcagg
     2701 gcgagacagg tctcgtgatc cacgtcgccc aggaggagca tcgcgttttg caggccttgt
     2761 ttttcgatag actgttcagc ctcggtctgc tcttccccgc tccccatgac caggcagccc
     2821 aggcgcggat agcgggagcg caggcgggag acagcctcta caagcagccg aaagccgtat
     2881 tccggccgga agaacagcgc cgttgaaatc acgggcgaat gttcgcggat ccaggaaagc
     2941 aaccagtcct cgatgccttg cttgcgtgga acagcgccaa gataggcggg taggatttcc
     3001 gttcgctcta tgggcacgcc cagggaaagc agcgcagcac gaatctcggt ggtgacgcag
     3061 acaatccggg tgaagcgggc acaggcgaac gccgccagcg cccgtcgcca ggcggaggcc
     3121 gttcccaggt acttggcaac catgcccgag tgcaacgtca aaatgcggcc tgctgccgcg
     3181 ctgccggaaa cgccgccgat ggccgcgacc gcccagcttt tccagttatg gccgttgata
     3241 tgaacgtgaa taatccagcc ctgtttcgcc cgacgccgca gcgaccggat cagccgcgcc
     3301 caatggcgca ggcgtgcggc gcctttggcg cgcgaaggct ccggcaggtt cagcacctgg
     3361 caggacaggc ccgcttccgt cagcagcttg tgagccgcca gcacatgcac cgaaacgccc
     3421 ccgtagggcg gcgggtacgg tcccatcaaa agaactttca ggcgcacagc ttggcctcca
     3481 ccaaccgcga ataaagcttt tcggtttcct ggacggcgcg ctcgatggaa aacagctcgg
     3541 ccaccctgcg ccgaccgttt tgaccgaagc ggaaagcttg ctcgcggtct tccagcaaac
     3601 ggccgatagc ctccgccaag ctcgccgggt cccgcgccgg taccagccat ccagtgactc
     3661 cgtcttcaat cacctccggg tttccgccga cgcgcgtcgc caccacaggc actcccgctg
     3721 ccatggattc gagcaacgag tttgaaagcc cctcgctgag cgaagggagt acggacaccg
     3781 ctacttccga caacagctca ggtacatcgc tgcgaaaacc cgtgaaaatg acccggcgtc
     3841 cgactcctac acgccgcgcg tggtcttcca attcctggcg gatgtcgccg tcgccgacca
     3901 cgagaaacgc aacgtttgga aacttggccg cgagaatggc ggcggcgtcc agaaagtatt
     3961 caattccttt cattcgattc agccgcgaag agactgcgac caactgcgcc gaggccggaa
     4021 gcccgagctg caagcgaagg agatggccgt tttgttttgc ggcgaatcgc gaaagcatga
     4081 tgccgttgcg aatcacgaaa atcgtttcgg gccggtagcc ctgctcaatc agggttctgc
     4141 gaatcgcttc cgcgttgaca agcacgcaat ccgccatccg gcataccgcc ttttgcaccc
     4201 gcctttgcat gggcgtcatg acttctccgg tgtcgcggat ggaagccacg accacacatc
     4261 ggccggccat tctcgccgca ggcacggcga agacgttcga gtaaaaaccg tagctgtgca
     4321 cgacctggat caagttccgc cggatgtatt gagcgaaccg cgccgcctgt aaaaaagtct
     4381 tgccgtcgta aaactttccg atttggaact cgggctgcgg gacatcgagc gtttccaaat
     4441 ctttcagcag ttcgccatgc cttctgagcg atcccaaatg aagctcgaac cgcgaactgt
     4501 ccagcctcaa cgctacatta gcgacctgcc tttccgtccc tccaatcttg aaattcgtga
     4561 ccagcttcaa aagcctgatt ttttgtaact gcatacgaac gccgcttcag gccgcaacct
     4621 cggtccttgc gccgcgatcc aaaattgccc gggaccaaag ctcaaacgag atcagagtcc
     4681 agagctgcaa atcaaaatcg cggcctttct catgcagatg cagaattttt tcgacgtact
     4741 gctggttgaa caccccgcgc cggcggctgg gagccgaaag gaggagctcg cgggcatagt
     4801 tgcttagcct gtttcggaac cagtaacgga gcggcacggc gaatccgtgc ttgggccggt
     4861 taatgatttc atccgggaga attccgcgca tcgcccgttt cagaatgtac ttagtcgtcc
     4921 cattccgcat ttgcatttcc ggtggaatcg ttgcggcaaa ctccacaagt ttgtgatcga
     4981 gcaaggggac tcgggtctcg atggaatgcg ccatgctcat gcgatccact tttgtcaaga
     5041 tgtccagagg cagatagcgc tggatatcga ggtattgcag gcgcgtgagc cagtggcggc
     5101 cttccagccc atccggctcg gcgccgcggc cgggatccgg ttccggcgag agcatggcaa
     5161 agatttctgg ctcgaagagg cggcgcagtt cttcgcgccg gaaaaaagtg gacgcgtcca
     5221 ggtagcgctc aaagcccgtg cgcgacgcgt ggcgcagaaa attccggccg cgcatgccct
     5281 ccggcatcgc gcggctgatg aacgccagca tttttctggc cggggcaggc aaaggcgccc
     5341 tgctcctttc gcgctgttcc actacgtact tgtcgtatcc ggcgaacaat tcgtccccgc
     5401 cgtcgccgga aagcacgacc ttcacgtgct gcgcggccat ttgggagacc atgtaagtgg
     5461 caatggccga tgaatcgccg aaaggctcgt ccagatgcca cgccagatgg tcaaggcgct
     5521 ccagaatgtc cggctggagc acgagttcgt ggtgatcggt atgaaaagct tccgccaccc
     5581 ggcgggccgc tcccaattcg ttgaactcct gctcggcaaa accgatggaa aaggtcttca
     5641 gcggctctgt cttttgcctg gcggccgagg ccacaaccga gctcgagtcg atgccgccgc
     5701 tcaaaaacgc gccgagcgga acatcgctga tcagatgcag gcaaacggat tcctcgaggt
     5761 gctcccgcaa gccctcgatg aattgcttct cggttttgcc gtaatccggc tcgaagtgca
     5821 ggtcccagta gcgggaaaca gccggggggc ggtctgccga agcggtcatc agatgggcgg
     5881 ggggcagctt gtgaacgccc gctatggcac tttcgttcgg gggcgtaacg agatacgaca
     5941 aacagtgcgc gactgcgtcc cagttcagcc tgcgctcgac ttcctggagc tccagaagag
     6001 ccttgatctc cgacccgaat aagagcctgc cgccgacatc ggcatagtag aggggcttga
     6061 tgcccagccg gtcgcgggcg aggagcagcc ggcgtttctt ttcgtcccac agcgcaaaag
     6121 cgaacatgcc gcgcatgtga tcgacgcacc ggtcgccata ttcctcgtat agatgaacga
     6181 tggtttccgt gtcgctgcgc gtatagaagc ggtggccgcg ggcgcgcagc cggatgacca
     6241 gctcccggaa gttgtagatt tcgccattga agacgaccca caccgagcca tcctcattcc
     6301 ggaccggctg acggcccgag tcgaggtcga tgatgctgag ccgccgcatt cccaggccca
     6361 cgccgctgcc aagataaaga ccttcgtcat ccggtccgcg atgcacaatc gcggagcaca
     6421 tggcgcgcac ttcatggccc aaaacgggct tgcctccgta gctgaaaatt cccgcaatgc
     6481 cgcacataga atccgcgctt agctgaaggc ccgggcggcg gctccgaccg cctgccggaa
     6541 ccatcccgtc acagccagct tctcctcttt gcgcagcaga ccaaaatgcc agagcatggc
     6601 ggcgtaagct gcgccgtaaa tcccggcggt ggctgccagc gccgtaaagg tgggcaggtt
     6661 ccggcccgtg gccaaaggaa ggtgcggctt cgctgccata gcgatcgtag ctgcggcaat
     6721 ggccgcgcac cacacggcgg cgaggttgcg ccagggcagc actcccgcga aatcggcgca
     6781 aagaagcttc ttgattctta taagcgccgc cgcctttccc gccagcgcgg ccagcatcgt
     6841 cacgagcacc gcgccgagaa gatagaattc agacagaaac cagccgacca gcgaggctat
     6901 gatggccagc cgcatcaggt tcatcgccag caaaaagcgg gtctcggcgt agacgcgcag
     6961 aacgccgtca atctgaaaca cggccagcat cactccggcg gaccacaccg caaacaccgg
     7021 cgcgctggcg gcgtatttcg ggaaagcgaa gacaatcatt tcctttgccg ccgccgccag
     7081 gaagcccgcc agcggaaaga agaggagagc cagcttgcgc gtggtctcgt gccagatccg
     7141 caggaccgcc tcctgcctgc cctccgcaag acgctcctgc atcttcacca tcatcacgtc
     7201 gctggtcggg ttggccacga aatcgacgaa cggaatctga acgcacccaa ccgcatagac
     7261 ggcaaacgtc gccgggtcga accggtgcga tacggcgtat tggtgcaagt tggactgggc
     7321 gatctccacg accaccgcca tgccgaaggg aagcgcataa agcagttgcc gcttgagcag
     7381 cccggcatcc ggcctcaggc cgccgccgaa ctcccggcgc aggtaccaca tcatggctcc
     7441 agcgcgcagc gcggccaccc ccacggcagc tttgaggacg gcgtcgaggc gcgcaacaat
     7501 cagcgccggg ataatgagag ccgccgcccg cgtcaaatcg gatagggcgt aggaaaccga
     7561 tgcccagagg tatctccccc gcgacaccat cacgatttcc agcagcgagg aaaccatcat
     7621 cagcgacagg aacagaccga tccagatcat gtacggctcg agccccggat tgctgaacca
     7681 gcgggcgagc cagggaccgc ccagggccag aaagcccagg cacgcgaggc cgctcgcggc
     7741 caggcagagc gcggagttgg ccacgaatcg gcctgcctct gaggcgaatc ggggcagaaa
     7801 aaagtacaag ctcgacgaca tgccgatttg agcgatcaag aagacggttg tgtaaatcag
     7861 gaagagctgc ttgtaggttc cgaattcggc gcgatcgaaa atcccggcca gaatgaccgg
     7921 aatgaatgac gtcgcggcga aggccatgat ccgccccgac atcagcagga gggccggccg
     7981 gaaaatggaa tccgtgcttc ggatctggcg atcggccgcc agggtcatga gttctgctcc
     8041 gcggcgcgct tcgccgaaca gatcagtccc gtcagaatgt agggaaacca gctcaatacg
     8101 taacctcccg atagtccgca aaccgcaaaa ccccagagcg cgatctccag gcccgcagca
     8161 agcggcgtga tgttccccgc cggcctgcgc cgggcgagtt tccgcgcagt agcaagaccc
     8221 gaaccgagga agaggatgaa aggaatgaac ccagccaagc cggtctcgct caacggctgg
     8281 atgaaggtat tatgagtcac cagttcgcct ctgccttgcc ggtagagatc ttccggcgca
     8341 tacagcggcc acgctatcaa cgagcagccg aagccaacgc cgagcagcgg atggtcggcg
     8401 aacatggcaa ggccggcgcg cgacgtagcc aaccgctgcc ggaaactgac gtcctggctc
     8461 aaatcggaaa aaccttcgtc ccgcgaccaa tagcggccgg ccgagaccag cgcaacggat
     8521 gccgcggcgg ccagcaggca cagcactgcg gcgctgcggc gccgccacgc gtacagcagt
     8581 aaagccgcga gcaggcccac catgccgccc cgggaaaatg tcaaaaagat tgcggcggcg
     8641 tagagcagag aaaatcccag cagcgccacc cgcccggtaa gacggcattt tgcggccagg
     8701 catcccgcca gtgggagcag gacaacgagg ctcgcggcca gttcgtttgg gttcgcaaaa
     8761 atgcccagcc aatgcgctct cccttccaca gtctggccag ccgcgtagta gcgcaacgct
     8821 cctgccgcgg gcgccaatcc gccgattagc atgacccaca tcaggcctcg cactctccgt
     8881 tcgctgtcca cgcagttcac gaccacgaag aaaatcagag caatcttcag aaaatccaga
     8941 gtcgattcga acgcaagacg cggccataaa gccgtagagc aggagagcgc cgccgctccc
     9001 aaaaagccca gcaaggcgta accctggggc caggccggat cgaaactgcg ccccgcaaag
     9061 acgacttcca tcgagagaat gatcagggcc gctatggccg taagctgcgc cggccgcacg
     9121 gcctccaggg cggggtattg gagagggagg ctggtataca gaagaaagac gaaaatgaga
     9181 agcagccaat aggaaatgga ggtggcgggg acggcgcgcc gggcgaactg gattgccgtt
     9241 ccgccgcctt cctccattgc caactccggc tggttatact tcggcatcca gcagcctccg
     9301 gacgattgtg tggttctggc ggcagcgctc gacaaagagg tcgaacacgc gatccagatg
     9361 gcagttcagc atggcggaag agaaagaccc atgagaatcg cggcaagcgt tttcccacag
     9421 caccgtcctc ggcaccgtca gcagaggatg aacatggttg cggtgcgaac agattgtata
     9481 ggcaaaacga taccccgcgc tggccacggc gtctacggaa atcgagtcga actgcccatt
     9541 ggggtaagca aagtggcgaa tcgggagccc caacctcttc tccaactcgc ggcgcgactc
     9601 ctgcgcctcg tcgagcacgc gctgccggct ttcccgcgtc atctgggcgt gcgtccggct
     9661 gtgggagccg atcaggatcc cggccttatg aatctcagcc agcatttccc aggtcaaaga
     9721 tcgaaagggc ttccgaacct cttcgggaat ctccgtttcc gaggcaagct tcgcaagcac
     9781 cttgtcgagc tcggcccgcg gcaggttttc gagcaaaacc cgcgtggctt caaaagcatc
     9841 cctcggtttg aaatgcggca gccggatttt gaggctttgg agcaatttta tgaagtgcgg
     9901 aggcggccat ttgtcgcggc cgaatctttt tgaaaccaaa agataaagtt tgtcgtgagc
     9961 gtgaagatct gccgtgccaa gagttgcggt cacgacaaat accgccgcgg ggatgccttt
    10021 cgcccgcagc aagggtaacg cgtagtcgta aaaatcgcgg tagccatcgt caaaggtgac
    10081 ggccgccatc ggcttgccgc agcgggctcg gccctcgagc tccgcgccca gttcgtcgag
    10141 agagacaaaa cggaagcggc gtccgaacga atccaaatgc tgctcgaggg tgcggcggct
    10201 caccagcatc gaggggatgg atgtttcggc gctcgaggca aaattctcga ctacgcggtg
    10261 gtaggccaca atcaccggac tgttgcgcat ccgggaaaga acgcgaaaaa gctggcccat
    10321 gccgcttcgg gccagggccg cggcggcccc tgttttgacc agcgttcgaa tcatcatctg
    10381 cggctgctcc ggtgcgcctt cacggtcaag cggtttacga cggtttgcgg cagcacgcgg
    10441 cgggccactg ctctcgatgc acggccgatc tccagcgacg agcgatagag ccaaccccgc
    10501 gcccggggcg gatacaattc caacttcagg agatcccggg attgccggct ccagcggctc
    10561 ttatactctt cgccgccgtg aaggaagtca tattccaacg ccccttccgc gatggcgctt
    10621 ttgatggata agccgattgc cgccaggccc acgctgttct tgctataagc cggatcaaaa
    10681 ccggactgat aaaagtagaa cacgcggcga taaaggaatc cgtacacgcc cgctgcggga
    10741 gcgccatcca ggcgcagcaa atagaggcgc agccacccgc ggctgagggc gatctgcgca
    10801 aattcttcat gaaaagcaag cagctcggaa gtgtggaaag catcggagcc gccgcgcgcg
    10861 tcccatcgca gatgatgcag ccgaacgact tcctccagcg ccgggccaag cggtccttcg
    10921 gttttgatct cctcgaactg cgcatcaaat tctttctcca tacggcgcag cttccgccga
    10981 atgttgtaac ggtgctcgga ccccagttgc tcgaggtagc tttcccacga atgtccttcg
    11041 agggagatga acgggcatac attgcttacg ctcggcgacg cccgccagcc cttcgccgca
    11101 aagatcggga ttgcccgctc agccaaggac gcgccccgtt ttagctggct caattcgatc
    11161 atgagcagtt ccggcgccag cttgccagcg agagcgcgga gcgactccgg ctcgcacccg
    11221 cggcggatga tgaagtccaa ataatcggaa cctgcgaagc cggagccgag aaattcgagg
    11281 accggcagag gatgcgaacg catcaggctg ggcggcctca aggccagcgg agcgagaccg
    11341 gttagtttac cctcccggcg gaccgtcaag atccacagtt tccggtttgc gcgcaggtgt
    11401 ttccaccagg tatagagcca ctcccaggtc aggaacagac aattcacggc gcttgcctcg
    11461 agaagctcgt cccactcggt tctcaaacgt tcaaattcgt gaatgtcgct gacccgctct
    11521 acagccaggc attcaccgcc cgtctcggcc ctcgttgttg tcatatctcc acgaaatctc
    11581 ccgcgagctt ttaccgggcg gccgtgccgc cggaatgcca gacgtccatc cgcccccgcg
    11641 caaagttgta cagcccggcc acggcggccc aattcatcag gacaaacgtg taagaaagca
    11701 aggcaagttt tctccaaatg ccggtcatat ctgcctttgg ttccttccgc actcgatttc
    11761 agccggcgta cccggcgggc ggcgtctcgc ggcaagggcc ccgattgccg ccagggcgta
    11821 ccaaaaaagc tggagcagaa aagcggcccg gtagattcct tgctccagcg gaagattcga
    11881 aacaaacaac ccgacgagaa aatagggaac gagcagccgg cccgccttgt gggaaagaaa
    11941 ctgaaggaat accggattcc gccagggcgc gagcagcgcc ggcatttccg ccagcagctg
    12001 gtagtttcct gtcagagtgc gcgcttttct tgcatactcc gccttcgggt tcgcggccgc
    12061 caaatcatag gcgcgggcgg aggagtcaaa cacgacgcgc ttgcctccca gcacgatccg
    12121 cataggaatc agcacgtcat ctaagatcgt cccagggggc aacggcgtga aaagctctcg
    12181 ccggatcgcg taaatggcgc cggtcgcgcc gatcaccgaa tgcgtggcgc tttcgagcgc
    12241 gcgcagccat ttctcatacc gccagtagac gccgattgcg ctagatgctt ccctgcctga
    12301 ttcttcgagc agcaccagct cgccggagac cgcgcccact tccggatccg agaagttcgc
    12361 cgccagctct cgaaccgcgt cccgatgaag ctgctgccgg gcgtcggtga acacgacgat
    12421 ctccccggtg gccgcctcca tcgcggcgtt cagggccgca gccttgccgc ggtgcacggg
    12481 cgagaaaaca atttccacgc ccctcgacgc atacggccgg acaacggcct cgacgccgtc
    12541 ggttgggcca tccagagaca caatcacttg catcttctcc gccgggtaat ccaactccat
    12601 gcagttgcgg atcttccttt cggcattggc cctctcgttg cgcatggcaa tgacgatgct
    12661 taccgggggc tccccataac tcttatggac cctcctcggc gccgcaagct tccacgctgc
    12721 cagcaacaga gggtatccga agtacacata cgcgataaag atcaccgaaa tccaaaaaac
    12781 agcctctgcc atagaaggat gcttctattc caccgtcacg ctcttggcca gatttctagg
    12841 tttatccaca tcgcagttgc gcagaacggc ggcatggtaa gcgaagagct ggagcggcaa
    12901 tgccgccagg agcggataca gcagatcatt cgtgtaaggc actgggatcg tgtgcggagt
    12961 cagcttggcg atgtctccgt cgttttcggt aaccaccgcc agaagctttc cgccccgggc
    13021 ggcgacttcc tcgatgttgc caagggtctt cttgtaaacg gagtctcgag gcgccagcac
    13081 cgccacgggc atatcagcgt cgatcagcgc aataggaccg tgcttcattt cgccggccgg
    13141 atagccttcg gcgtgtatat aggagacctc tttcaacttc agggcgccct ccattgccag
    13201 gggatagttc actccgcggc ccaaatagag gaaactgcga tacgagcaga actgctgcgc
    13261 gagcgactcg atcgagcccg atcgattgag aatctcctgc atctgatcgg gtatctgttc
    13321 gagcgcgcgg acgcgaacac gaacctgctc gggcgggagt gtgcgccggg cctgggccag
    13381 ccacagcgcc agcagataga gcaccgccaa ttgagtggta aaggctttgg tcgatgccac
    13441 gccgatttcc ggccccgcgc gagtatacag cacgttttcc gcctgccgcg ccgccatgct
    13501 gccgaccacg ttgcaaatcg ccagacgcgg cgctcgcctg tcgccagctt cgcgaagggc
    13561 ggccaacgta tcggccgttt cgcccgactg tgtaattgct acgaccatgc tgccgcggcc
    13621 gatcagcggg ttgcgatagc ggaactcgga ggcgtagtcc acttccacgc ggaggcgggc
    13681 caaattctct atgaaaaact tccccactag cgccgcgtgc aaggatgttc cacaggccac
    13741 tacgtgcatg cgctcgagtc cttcgaatac ttcgcggcgg ctgatttcgt cgtccaggtt
    13801 cacggactgg ccgttcttaa ggcggccgca aagagtttcg cgcagagcac ggggctgctc
    13861 aaagatttct ttcagcataa agtgcggaaa accattcttc tccgccatct ccgagttcca
    13921 ggcgatcggc tgcggccggc gtgcgacgct ggaaccggcc agggtccgga gttccataga
    13981 atcgggccgg atgattgcca tctcgccgtc gtccaaaaag agcatgtcgc gcgtgtaggg
    14041 gagcagggcc ggagcgtccg aggctacaaa gctctcctgc tctccaacgc cgaccaccag
    14101 cggcgctccc cgccgcgcag ccacaatctt catgttgtct cgcgtggaaa tcacgaccag
    14161 gctgtacaaa ccgtccactt gctgcaacgc cgcgcgcacc gcctcctcca ggcacccatt
    14221 gaaatgggcc tcaatgaggt gcggcagcac ctccgtgtcg gtttcggtcc ggaagacgtg
    14281 gccctcgagc ttgagctcct cgcgcagaga aatatggttt tcaataatgc cgttgtgggc
    14341 caccagaatg tggcccgtgc agtcccaatg aggatgcgcg ttgatttcgc tcggacggcc
    14401 atgagtagcc cagcgcgtgt gtccgatgcc gcatttgaaa cgggaggaag atttcggcag
    14461 agccagcgcc ttttcctcaa gcatcgccac tctacccgcg gagcgcagga ctttagggga
    14521 gccattatcg agtaaagcga tgccgcagga atcgtagccg cgatactcga gtctcttcag
    14581 accttccaac agcacctgcg gagcatcccg cgtgcctatg tagccgatga tgccgcacat
    14641 tgtattttcc tcctcacgca gccgcgctgg acgagaaccc gccgaagccg tgcaaagctc
    14701 cacgcgcgag cgacatgacg atatgaccca gcgtggccgg gtcatggcgg atcttttctt
    14761 cctgaggcga gagcaggtcg gcgcagatcg gcatggcccc cagccgttcg atctcccgcc
    14821 aatcacaagg caccggctgg cagcagtctc tacggccggt tagcgtcctg gcggccggcg
    14881 cgagccggga gttgacaatg cagaaatccg ccggccgcgg ccccaaataa gctgcgagcg
    14941 tgcgcaaatg atctgcggcg gaaaatccgt cggtttctcc gggctcggtc atcaggttac
    15001 atacgaaaat cttgagcgcc cgcgaccggc cgatagcctc ggctacgccg gcgaccagaa
    15061 gattcgggat caagcttgta tagaggctgc cgggtcccag caccagggcg tcggcatggg
    15121 tgatcgcctc caataccccg ggcgcgggcg aaactccgtc cggctcgagc caaacttgct
    15181 cgatgcgtcc accggctgcc ccaatttcag attcgccgcg cacgatggtc cgatcgtgta
    15241 gccgcgcgca gagcgttacc ggagcttcgg tcacgggaag cgcttcgccc ttcagcggta
    15301 gcagctggcc cgccagcgcg gccgctcggc ccagacttcc ggatttctgg cagagggcgg
    15361 tcacaatcag attgccgagc gcgtggccgg ccagcccgtt cccatctgag aatcggtgct
    15421 gaaacaactc ggcgagcacg gaatcgtccg gcgagagcgc caccaggcaa ttgcgcagat
    15481 cgccgacagc cggaatgttg aagcttcgcc gcaggcgccc gctcgacccg ccgttatcgg
    15541 tgacacaaac gatcgccgaa actttgacat cgacggataa acggctgtgg ctgccgtccg
    15601 cgaatggctg caaaccacga agcagaaccg gcaatcccgt gccgccgccg atgcccacga
    15661 tgctgagtct ggtcattatc gtcctcgccc gaacagcacg atgcccaagg tgcgcagcag
    15721 aatcactgca tcgcagcgca gagacatatg ctttgcgtaa taaaggtcgt attgcagctt
    15781 ttcgtaggcg tcttcgatag aggctccata cgggtactcg atctgcgccc agccggtgat
    15841 ccccggcttt acgcaatgcc ggagatcgta aaacggaatc ctaggccgga gcatttgcac
    15901 gaagtaaggc cgctccggac gcggccctac aaagctcata tccccgcgca atacgttgaa
    15961 ggcttgcgga atttcatcca accggaactt gcgaaggatt ttcccaacgg tggtgactct
    16021 actgtcgtgc tcgccggccc aaacagggcc ggtttcggcc tcggcgtcct ggcgcatgga
    16081 gcggaatttg taaagaacga acggtttgcc atgctgcccc acccggacct gccggaacag
    16141 caccgggccg ccggagtcca gccggatagc ggcggcgaca atcgccagca caggcgatgt
    16201 gagcagcagc aggagagcgg cgaacagcag atcgagagaa cgcttcgcgc aaacggtcaa
    16261 ccgggagcgg tcaaatccgt cggtgtagac gagccattgg ggatgcaatc cctccagcca
    16321 gatcttgccg ctcagcttct cgtagaaatc caccgcgtcg acgacgcgca aacctcgtaa
    16381 gcggcagtcg agcagggccg ccgccacttc ggtgcgccgc tgcgaatccc gctcggcgac
    16441 gatcacgcaa gaaatccgct cccgggccgc aagttccggg atctgatcgc aatcgatgac
    16501 cgctgcgccg ccccgcggcg ccgcaggaaa ctcgatgg
//
LOCUS       DASREK010000156         8036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2222110, whole genome shotgun sequence.
ACCESSION   DASREK010000156 DASREK010000000
VERSION     DASREK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222110"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="VFA54_07840"
     CDS             <1..1085
                     /locus_tag="VFA54_07840"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZS50755.1"
                     /translation="ILTRNHRGRPACHWCGHCGRGCTTGSYYSSPASTIPAAFQTGRL
                     TLQPDSVAHEILVDGNGLARGVAYIERLTNRSREVYGKTVVLCASTLESTRLMLLSRS
                     RFHPNGIGNSSGVLGHYLMDHTIGCGAAGFLTRTAGPVDRWDDGRANGIYVPRFRNLK
                     TKRPDYIRGFGYQGGGGRGMFPTHAERTPGYGAAFKRAVRERWPYPLRLFTWGECLAR
                     YENHVRINYEKKDRWGVPVLHIECAFGDNELKMMKDAVDTAQEMLLAAGAEITEVNYT
                     PRPPGTCIHEIGTARMGTDPKTSVLNKWNQSWDVKNLFVTDGASFPSSGCANPTLTMM
                     AVTARACEYILDQYKKGFKDSPKANA"
     gene            1152..1340
                     /locus_tag="VFA54_07845"
     CDS             1152..1340
                     /locus_tag="VFA54_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50756.1"
                     /translation="MGNAEHDRRIAYIEFPASGIAEAKEFDSMIFGRRFEDYGPDYEL
                     PRRAAGRRVLQASASARL"
     gene            complement(1494..2033)
                     /locus_tag="VFA54_07850"
     CDS             complement(1494..2033)
                     /locus_tag="VFA54_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50757.1"
                     /translation="MPGAGKKPSRKSVFEEYIRANPPAEINRQTLAELRDSISQRLGG
                     AAVSERYLLELIEHTAVPISRELGGLPPDLRNRVRFHDFASAEASLYDLQKEYEDARR
                     AGDRLRAQDCRRAVLRGRQRLELLLRRPRWRAEKRAEKIEILGWFRVWLENPELFRTW
                     LELRKRAIDWAQGKTETGD"
     gene            complement(2017..3057)
                     /gene="mltG"
                     /locus_tag="VFA54_07855"
     CDS             complement(2017..3057)
                     /gene="mltG"
                     /locus_tag="VFA54_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZS50758.1"
                     /translation="MLRRAALLIFFFLAGALFYTAYSILKPYRAYEGEVFVDIPRGAG
                     TLEISARLRSAGVLRSEWPFWLIRLSRSRGMLKAGEYRFDRPLSPLQVYRKIASGEVF
                     YYSLTIPEGYDMFQIAEAVARTGIMNQQQFLEAARHGERVAAFAPGAKTLEGFLFPDT
                     YRLTRQTTAEELVGQMVRRFRDVFREVQGEHPPQTQQVLETVTLASLVEKETPVAAER
                     PMVASVFRNRLRIGMPLQCDPTVIYALELAGRYTGEIRKEDLAFPSPYNTYLHPGLPP
                     GPIASPGRASIEAAVNPAKTDYLYFVADNKGGHVFSANLQKHSQAVASYRRANGRRPG
                     PAQSAKKDARRR"
     gene            complement(3057..3500)
                     /gene="ruvX"
                     /locus_tag="VFA54_07860"
     CDS             complement(3057..3500)
                     /gene="ruvX"
                     /locus_tag="VFA54_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZS50759.1"
                     /translation="MAVQAGRILALDLGARRIGLAVSDELGWTAQGLPTLLRRNTQTD
                     LDSLAAVIRENNVGLVLVGNPLRMGGGESAGTLQAARFAGQLKRRTGVEVRLWDERLT
                     TREASRVLRSSGVSISKRAKAIDRLSAILLLENYLEHESAARKQP"
     gene            3747..4367
                     /locus_tag="VFA54_07865"
     CDS             3747..4367
                     /locus_tag="VFA54_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50760.1"
                     /translation="MSAPNSTDSGLSGNATPDVIGCGHVTTTPPDGHDGDSHVHHGQH
                     GHTITPGTECLPPGHHVLVNGHILGLTPEPGEGFFHLPRPPAREPDFVAALNEDGGTG
                     PASRGTILQLFGSARGLFLDLADQKPALNFAPPASGTLLYYTDTLPEVSIGGIAAQVL
                     FSGLAPGLTGVWQINVRVPDAVSPGRAPVSIVYNGAVLQSIDVAVR"
     gene            complement(4469..5722)
                     /locus_tag="VFA54_07870"
     CDS             complement(4469..5722)
                     /locus_tag="VFA54_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS50761.1"
                     /translation="MTRAEIHESLLIALDGIRSRKMRSFLTVLGVVIGVTSVIAVAAI
                     IDGLNRNILERVQALGARTFFVSRLPVARFGRIPEEIRLRKYLNVSDAQVIRESCPAV
                     AVASAFATRATFTGDVNDVRYGDQRVTNSILRGVDADYGAAVPAFDVTNGRFISGYDM
                     DHVRFVAVIGQGIADSLFLNSDPVGKLLRLNGLPFEVIGVFGKDSGFLGAPGVDSFIC
                     IPQSTFQRLYPEFREYNIAVSVHDAKDLPAAEDEVVTALRRRRRVKPSAPNDFEITMP
                     DFLSNLWNQLTGALVILTTVISSIALLVGGIGVMNIMLISVTERTAEIGVRKALGARR
                     NDIRAQFLIEAMTLTSVGGAMGVLLGALVSWVVRTFVPSLPTYVSPFWVAAGLLMSAG
                     VGLFFGFYPANRAAGLDPIVCLRHE"
     gene            complement(5802..7028)
                     /locus_tag="VFA54_07875"
     CDS             complement(5802..7028)
                     /locus_tag="VFA54_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS50762.1"
                     /translation="MPGLRTNVTLAAASIRAQRLRSFLTVLGITMGVATLMAVITLVQ
                     GANTYVEQKIANLGTNVFQVSKYPFATTNFDELIRSRRNRDITLDDLEAIRRACLHCE
                     AVGGQAEAVAHVRYKDQELQDQTFYGHTANMSVIGSRTLESGRYFTDLEERHMASVCL
                     IGDSVRTKLFAGTNPIGQVVRVGKDDLIVIGVFERIGAVLGQEQDNFVVAPLPLFLRT
                     VGIRRTVTINAKASGGEEIFQHAQDEARLVMRARHHITGNKREDFYIGTAESYIALWK
                     SITSSFFTVFLMLSSIAAIVGGIVIMNIMLVSVTERTKEIGIRRACGARQGDILRQFL
                     TESVLLCIAGGVAGVALGFLAALGVRTLADFPATVKFWAAALGVILSSVIGLFFGIYP
                     AVKAARLDPVAALRTE"
     gene            complement(7142..>8036)
                     /gene="tyrS"
                     /locus_tag="VFA54_07880"
     CDS             complement(7142..>8036)
                     /gene="tyrS"
                     /locus_tag="VFA54_07880"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZS50763.1"
                     /translation="ETYKAQVFKILDRERTEVRFNSEWLGEMKFIEDVIPLCSRYTVA
                     RLLEREDFAKRFREGQPISVHELLYPLTQAYDSVALQADVEMGGTDQKFNLLVGREIQ
                     RDYGQPPQIVATMPILEGLDGVQKMSKSLGNYIGITEPPEVMYRKVMQISDELMYRYY
                     ELLTDLSLAEIAQLKTEMHPMQAKKDLARRIVADFHSPEAAEKAAEEFRRVVQEKEVP
                     EEIPTVPLPNGARSAGVVRMNKLLPQIGLADSVSDATRKIKAGAVEVNGARHRDLGLK
                     LEGTAGQLLIQVGKKWRRVTW"
BASE COUNT         1563 a         2526 c         2442 g         1505 t
ORIGIN      
        1 ccatactcac gcgcaatcac cggggccggc cggcgtgcca ctggtgcggc cattgcgggc
       61 gcggctgtac caccggctca tactattcca gccccgcttc gacgattccg gcggcgttcc
      121 agaccggacg cctcacgttg cagcccgatt cggtcgcgca cgaaattctg gtggatggaa
      181 acgggcttgc caggggcgtc gcctacattg agcggctcac gaaccggagc cgggaagtct
      241 acggcaagac cgtggtgctc tgcgcttcga cgctggaatc gaccaggctc atgctcctct
      301 cgcggtcgcg ctttcatccc aacggcatcg gcaactcgag cggcgtgctc ggccattatc
      361 tgatggacca cacgattggc tgcggcgccg cgggttttct taccagaacc gcaggccccg
      421 tagacaggtg ggatgacggc cgggcaaacg gcatctatgt gccgcgcttc cggaacctga
      481 aaacgaaacg cccggattac atccggggct tcggctacca ggggggcggc gggcgcggca
      541 tgttccccac gcacgccgag cgcacgcccg gctatggagc ggctttcaaa cgggcggtgc
      601 gcgagcgctg gccatacccc ctccgcctgt ttacgtgggg cgagtgcctg gcgcgctatg
      661 aaaaccacgt gcgcatcaat tacgagaaga aagaccgctg gggcgtcccg gtgctgcaca
      721 tcgagtgcgc gttcggcgac aacgagttga aaatgatgaa ggacgcggtg gataccgcgc
      781 aggagatgct gctggccgcc ggtgcggaaa ttaccgaagt gaattacacg ccccggccgc
      841 cgggaacctg cattcacgaa atcggcacgg cgcgcatggg aacagacccg aagacttccg
      901 tgctgaacaa gtggaaccag agctgggacg tgaaaaacct ctttgtcacc gatggcgctt
      961 cgtttccctc gtcgggatgc gcgaatccca cgctcaccat gatggcggtc accgcgcggg
     1021 cctgcgagta catcctcgat caatacaaga aggggttcaa agactccccc aaggcaaatg
     1081 cgtgaagcgc cggtgatcgg gcggcttcgc gatcatttat ctcttgcaac gacaatcttt
     1141 caggagagcg tatgggtaat gccgagcacg accggcgcat cgcttacatc gagttcccgg
     1201 cctccggcat cgcggaggcc aaggagttcg actcgatgat tttcgggcgg aggttcgagg
     1261 attatgggcc ggattacgag cttccacggc gggcggctgg ccggagggtt cttcaagcct
     1321 ccgcaagcgc gcgcctctag gccgctgatc gcgatctgct gggctgatct ggaaaaaatg
     1381 gaagcgaaca taacggctgc tggcggcaaa attatgaaat ctgtgttccg ctttcccggc
     1441 ggccgccgtt tccacttctc gaacggcaat gaactggccg tggggccgga tatttaatcg
     1501 ccggtttccg tcttcccttg cgcccagtcg atcgcgcgct tcctcaactc gagccaggta
     1561 cggaacagct cggggttttc tagccatacc cgaaaccagc ccaggatttc gattttctcg
     1621 gcgcgttttt ctgcccgcca ccgcggccgc cgcaacagca gttccaggcg ctgcctgccc
     1681 cgcagcacag cacgccggca atcttgcgct cgcaaacggt cgcccgctcg gcgggcgtct
     1741 tcatactctt tctgcaaatc atacaggctc gcctctgccg aagcgaaatc atgaaagcgg
     1801 actcggttcc gcaggtccgg cggcaagccg cccagctcgc gagagattgg cacggcggtg
     1861 tgctcgatta gctccagcag gtagcgttcg gaaaccgccg cgcccccgag ccgctgagaa
     1921 atggagtcgc gcagctcagc cagagtctgc cggttgattt ccgcgggtgg gtttgcccgg
     1981 atgtattcct cgaacactga cttgcgcgag ggcttcttac cggcgccggg catctttctt
     2041 ggcggattgc gccgggcctg gccgccgacc attggcgcga cggtagctcg caacggcctg
     2101 agaatgcttc tggaggttcg ctgaaaatac gtgtccgccc ttgttgtcgg cgacgaagta
     2161 aagataatcg gtcttggcgg gattcaccgc ggcttctatg gacgcccggc ccgggctggc
     2221 gatgggacct ggcggcagcc cgggatgcag ataggtattg taaggacttg gaaaagccag
     2281 atcttctttc cgaatctccc ccgtataccg gccggcgagt tccagcgcgt agattaccgt
     2341 agggtcgcat tgcagcggca ttccgatgcg cagccggttg cggaacaccg aagccaccat
     2401 cggccgctcg gccgcgacag gcgtttcttt ctcgaccagc gaagccaaag taacggtctc
     2461 cagcacctgc tgcgtttgcg gtggatgttc gccttgcacc tcacggaaca cgtctcggaa
     2521 gcgccggacc atctggccta ccagttcttc ggccgtggtt tgccgggtca gccggtaggt
     2581 gtcgggaaat aaaaaacctt cgagcgtttt ggcgccgggg gcgaaggccg ccacgcgctc
     2641 gccatgccgg gcggcctcca gaaactgctg ctggttcatg atgcccgttc gggctaccgc
     2701 ttcggcaatc tggaacatgt cgtagccttc tgggatggtc agcgagtaat agaaaacctc
     2761 gccggacgct attttccggt agacctggag aggcgaaagc gggcggtcaa agcggtattc
     2821 gccggctttg agcataccgc gcgagcgcga aaggcggatc agccaaaacg gccattccga
     2881 tcgcaggact ccggccgacc gaagccgcgc cgatatttcc agcgtgcctg ccccgcgcgg
     2941 gatgtccaca aaaacctctc cttcgtaggc gcgatacggt ttgaggatgg agtaggctgt
     3001 gtaaaaaagg gcgccggcga gaaaaaaaaa gatcagcagc gcagcccgcc gcagcatcag
     3061 ggttgcttcc gggcggcgct ctcgtgttcg agatagtttt cgagcaagag aatcgctgac
     3121 agccggtcaa tcgccttcgc gcgcttagag atgctgacgc cgctcgaccg cagcacacgc
     3181 gaagcttccc gcgttgtaag ccgctcgtcc cataaccgca cttcgactcc ggtgcgccgt
     3241 ttcagttggc cggcgaaccg cgccgcttgc agtgtgccgg cgctctcccc gccgcccatc
     3301 cgcaacgggt tgcccacaag caccaagccg acattgttct cgcgaatcac ggcggccagg
     3361 gagtccagat cggtttgcgt gtttcttctc aggagcgtag gcaggccctg cgccgtccat
     3421 cccagctcgt cgctcacggc cagaccaatc cggcgcgccc ctaaatcgag cgccaaaatc
     3481 ctgccagctt gtactgccac acttcaatta taccgggcgc agtggagggg cgatcttcac
     3541 gatttcgcga gcccgcacgg gtgagagaga gcgttaacgg gaagggtaaa gttggatccc
     3601 ccggccatcg ggatggccca gactggacgg tgtgatcggg gcaggctcct ccgccgccta
     3661 gcgacacggt gccgtttccc tgggcgatga tggcggggtt gccgttgtcg aatgtggcaa
     3721 ggatcaggcc cggattcact ccggtaatgt cggcgccaaa ctcgacggac tctgggttga
     3781 gcggaaacgc gactcccgac gtgatcgggt gcggacatgt caccactacg ccgcctgacg
     3841 gccatgacgg cgacagccat gtccaccacg gccagcacgg ccacactatc acacccggaa
     3901 ccgagtgcct gccgccgggc catcacgtgc ttgtgaacgg ccacattctt ggcttgacgc
     3961 cggaacccgg cgagggcttc tttcacttgc cccgcccgcc ggcgcgcgaa cctgatttcg
     4021 tggccgcctt aaatgaggat ggcgggacgg gccctgcctc tcgcggaaca atcctacagc
     4081 tattcgggtc ggccagaggc ctgttcctgg acctcgccga tcaaaagccg gccctgaact
     4141 tcgcgccgcc ggcttcgggg actctgctgt attacacgga cacccttccg gaggtcagca
     4201 tcggcggcat cgccgctcag gttctcttca gcggcttggc ccccggcctg acgggcgttt
     4261 ggcagatcaa cgttagggtt cccgacgccg tatcccccgg ccgagcgccg gtgtcgatcg
     4321 tttacaacgg cgctgttttg cagtctatcg acgtcgccgt cagataaagt ttcgcttccc
     4381 gtgcaggaag cggcgagacc gtgagcacag gctctctccg ccacggaaaa gaggccgcct
     4441 gtcgatcccg catggggccg gggcgccgtc actcgtggcg caagcacacg atggggtcca
     4501 ggcctgcggc gcggttggct gggtaaaagc cgaaaaagag ccccacgcca gcggacatga
     4561 gcagcccggc tgcaacccaa aagggagaaa cataggtagg cagcgaagga acgaaggtcc
     4621 gcaccaccca actgaccagg gccccgagca gaactcccat agcgccgccg acgctggtga
     4681 gcgtcatcgc ctcaatcaaa aattgggcgc gaatgtcgtt ccgccgggcg ccgagcgctt
     4741 tgcgtacgcc gatttcggcc gtgcgctctg tgaccgagat cagcatgatg ttcatcacgc
     4801 cgatgccgcc gacgagcagc gcaatcgagc tgataaccgt cgtcaggatc accagagccc
     4861 cggtcagttg attccagaga ttggaaagaa aatcgggcat ggtgatttcg aagtcgttcg
     4921 gggccgaagg cttgacgcgc cggcggcggc gcagcgccgt tacgacctca tcttcggcgg
     4981 caggcaagtc cttcgcatca tgaacggaaa cagcaatgtt gtactcccgg aactcgggat
     5041 aaagcctctg aaacgtggat tgcggaatgc atataaagct gtccacgcct ggcgctccca
     5101 gaaaacctga atccttgcca aagaccccga tcacctcaaa cggcaatccg ttcaggcgca
     5161 gcaattttcc aaccggatcc gagttgagaa acagagaatc ggcgatgccc tgcccgatca
     5221 cggcgacaaa gcgcacgtgg tccatgtcgt agccggaaat aaaccggccg ttagtgacat
     5281 cgaatgccgg cactgcggcg ccgtagtcgg cgtccacccc gcgcaggatc gagttggtca
     5341 cccgctggtc tccataccgc acgtcgttca cgtcgccggt gaaagtggcg cgcgtggcga
     5401 aagccgaggc tactgcgacc gccgggcagg actcccggat cacttgggcg tcggagacat
     5461 tcaggtactt ccgcagccgg atctcctcgg gtatacggcc aaaccgcgcc accgggagcc
     5521 ggctcacaaa gaaggttctc gcgcccagag cttgcacgcg ctccaagata ttccggttca
     5581 atccatcgat gatggcggcg acggcgatga cagaggtgac tccgatcacg acgcccagca
     5641 ccgtaagaaa cgagcgcatc ttgcggctgc gaatgccgtc caaagcgatc agaaggcttt
     5701 cgtgaatttc agcgcgagtc atggagacca gatcgagcag ccctagattc gctgtcagcc
     5761 attggcccgg ccaggacaga gcccggatcg cttgcccatc tttattccgt tcgcagggcg
     5821 gcaaccggat cgaggcgggc agctttgacc gccggataga ttccgaaaaa caggccaatg
     5881 actgaggaca gaattacgcc cagcgcagca gcccaaaatt ttacagtggc cggaaagtcg
     5941 gccagcgtgc ggacccccag agcagccagg aacccgagag ccacgcccgc cacgcctccc
     6001 gctatgcaca gcagaacgct ttcggtgaga aactgccgca ggatgtcccc ctggcgggcg
     6061 ccgcaggccc ggcggatgcc gatttctttg gtccgctccg tcacgctcac cagcatgatg
     6121 ttcatgataa cgattccgcc cacgatcgcc gcgatcgagg agagcatcag gaaaaccgtg
     6181 aagaacgacg aggtgatgct cttccacagg gcgatgtagc tctcggccgt gccgatatag
     6241 aaatcctcgc gtttgttgcc cgtaatatga tggcgggcgc gcatgaccag gcgggcctcg
     6301 tcctgggcgt gctgaaaaat ctcttcgccg ccgctggcct tggcgttgat ggtcaccgtg
     6361 cggcgaatcc ccactgtccg caggaacagc ggcagcggag ccaccacgaa attgtcctgc
     6421 tcctggccga gcacggcgcc gattctttcg aacaccccga tgacaatcag atcgtctttg
     6481 cctacgcgca caacctggcc gatgggattc gttccggcaa aaagcttggt tcgaacggag
     6541 tctccgatga gacatacgct ggccatgtgc cgctcctcca gatcggtgaa gtagcggccg
     6601 ctctcgaggg tgcgggagcc gatcacgctc atgttggccg tgtgcccgta aaaagtttga
     6661 tcctggagct cctggtcctt atagcggacg tgagccacgg cctccgcctg ccctcctacg
     6721 gcctcgcagt ggaggcaggc ccgccgtatg gcttcgaggt cgtccagcgt aatatcgcgg
     6781 tttctgcgcg aacggatgag ctcgtcgaag ttcgtcgtgg cgaacgggta tttactcacc
     6841 tggaaaacgt tcgttccgag attcgcaatt ttctgttcga cataggtgtt ggccccttgc
     6901 acgagggtga tcacggccat cagcgtggcc actcccatcg tgatgccgag gacggtcagg
     6961 aaagagcgca gccgctgcgc ccggatcgac gcggcggcga gcgtcacatt cgtgcggagg
     7021 ccaggcattt cattccatca caacagcacc caggcgtcca acggcttgac cggtttgcgg
     7081 gggccgacgg gccacgcgcg tctcgcctgc gtcgaaccgg ctgcaagaag tccgggcatc
     7141 cctaccaggt tacccggcgc cacttcttgc ccacttggat gagaagctgg cctgccgttc
     7201 cctccagctt cagacccaga tcccggtgcc gggcgccgtt gacctctacc gcgccggcct
     7261 taatttttcg ggtcgcgtcg gacaccgaat cagccagacc gatctgcggc agaagcttat
     7321 tcatgcgaac cacaccggcg ctgcgcgcac cgttgggcag cggcacggtc ggaatctcct
     7381 ccgggacctc cttttcctgc accacgcgcc ggaattcctc cgccgccttc tcggcagctt
     7441 cgggcgagtg gaagtcggct acaatgcggc gcgccagatc cttcttggcc tgcatcgggt
     7501 gcatttccgt tttaagctgc gcgatttcgg cgagactgag atcggtcagc agctcgtagt
     7561 accggtacat cagctcgtcg ctgatttgca tgaccttgcg atacatcact tcaggaggtt
     7621 ccgtgatccc gatatagttg cccaacgact tcgacatctt ctgcacgcca tcgaggcctt
     7681 cgaggatggg catggtggcg acgatttgcg gaggctggcc gtaatcgcgc tgaatttccc
     7741 gcccgaccag gagattgaac ttctggtccg tacctcccat ctccacatcg gcctggagcg
     7801 ccacggaatc gtaagcctgg gtcagggggt atagcaattc gtgaacggaa atgggctggc
     7861 cttcgcgaaa ccgcttggcg aaatcctccc gctcaagcag ccgggccacg gtatagcgcg
     7921 agcagagcgg tatcacatcc tcgatgaact tcatttcgcc cagccactcg ctgttgaagc
     7981 ggacttcggt tcgctcccgg tcgagaattt tgaatacctg cgctttatag gtctcc
//
LOCUS       DASREK010000157        28832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_572835, whole genome shotgun sequence.
ACCESSION   DASREK010000157 DASREK010000000
VERSION     DASREK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 28832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28832
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572835"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFA54_07885"
     CDS             <1..903
                     /locus_tag="VFA54_07885"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZS50764.1"
                     /translation="CQKIIRLLALSLVACAAGNAQTPPVFDGQAALDATRRVVSFGPR
                     RLGSAAHELVRQWLKTELRRLRCEVAEDAFTARTVLGPRPMTNLIAKFPGSSRRIVVL
                     SGHYDTYDRPGLRFLGANDGGSSTGILLQLARSVARIAHRDTIWIVWLDGEESLVNWS
                     GDDHTYGSAHLARRWRSDGTAGSILALINLDMIGDADLDLAYELNSTGWLRDLVWSVA
                     WRLGYSTQFPRDRPNAIADDHVPFAESGIAALDLIDLDYGPDNAYWHTVNDTMDKLSA
                     RSFGIVGQVLLETLRVLEQRGNGG"
     gene            1037..2212
                     /locus_tag="VFA54_07890"
     CDS             1037..2212
                     /locus_tag="VFA54_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZS50765.1"
                     /translation="MKITRRSFFRWAGGAAGMAASSVPARADAPADIRITDVRPLSIN
                     GRTLFVLVRASNGMFGLGECSPMNLPVLAANIQNVLKPRLLNKDPFQVEPLYDELLYP
                     NFKLGPMGAVSEAIAGVDMALWDLKGKILGVPVYKLLGGKYRDRQPVYFSYGRGSAKR
                     DTPESVAKLMAQAAERGFTGVKIRMDFGSAVLDAPDDPSEAFISAIRRAVGDYVDVAF
                     DANNGYTAKRAIAVGRRLYERYNIAWFEEPTPQYDYAAMAQVAAALEVPISAGEHEYT
                     IWQFKDLIAQSGISIVQPDVAKCGGLTQAKKIAALALAHNKHIVVHNTTPTVATAAAL
                     HFAASCSNAARRQEYPGERADLDRFFENKLEFDRGLLKIPELPGLGLVVRQSELEKL"
     gene            2317..2739
                     /locus_tag="VFA54_07895"
     CDS             2317..2739
                     /locus_tag="VFA54_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50766.1"
                     /translation="MTSCPTCGAILQGAAGCPTCGPVRTGFERWTAALAYFWITGIVF
                     LFAARRRANPYVRFHCLQAVFYGAVVLLMNAVLAIVAVQIGTMGAFPIIYATVWGVIR
                     IGLFLLWILGMLKAYEGIEYKLPWVGNLARKYVPARRA"
     gene            2802..3773
                     /locus_tag="VFA54_07900"
     CDS             2802..3773
                     /locus_tag="VFA54_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HZS50767.1"
                     /translation="MLRFAAILLALMTLTGDLSAQQKKRVAVLNFDYATVHSSVASIF
                     GSDVDVGKGVADLLVDDLVKSGVYSVIERKALDKILAEQNFSNSDRANPQTAAKLGQL
                     LGVDAIVIGSITQFGRDDKATNVGGGVLGGFGSKYGIGGVSKRQSKAAVGITARLVSV
                     ETGEILGVAEGKGESTRSGAGLLGAGGAGGTAAGGGYDMTSKNFAGTILGEAVNQAVA
                     MLARNLDQNAARLPSRKIAVDGLVADVSGSTLTLNVGSKAGVKVGDRLQIRRPVREIK
                     DPATGKVLRRVEDKLGEITISEVDENSSVGTYAGAAPKVGDRVAALP"
     gene            3786..5615
                     /locus_tag="VFA54_07905"
     CDS             3786..5615
                     /locus_tag="VFA54_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZS50768.1"
                     /translation="MYFQPGDTIGDYEILSVLGEGGMGKVYKVRNVISDRLEAMKVLL
                     PDLHGDPELADRFVREIKVQASLDHPNIAALRTALRAENQLIMVIEFVEGRTLVDLLE
                     RGPIPLYDGIEYMCQALCALAYAHERGVIHRDIKPANIMLANDGVIKLMDFGIAKAAA
                     DRKLTMTGTTMGSLYYMSPEQVKGTTALDARSDLYSLGITLYEVVTGTRPFQGDSDYA
                     IMSAHLEKTPVPPVEVDPNLPGALNEIILKSIAKDPADRFQNANAFRTALKSVQAELI
                     PEAAAFPGAQRQTPRTEAAPSPRLDTAPPVPPPMPSEAIPAAPRGSPPVARRSAPIPP
                     APSRRGFYMLAGGVLTLVLLIAAGIEVANWRKTRAGAIPETAPAGADEQNTRQPVNST
                     GGSQPEASPLGAARSTDAAGPAQPNTTPAGLAGQSGAAAVAGAAATDSGSRKAGAGQD
                     AAFSAAPTPAPQAGRVAREPGRTGQAALRSVPQQAQQPANRTPKAAAPDLVLPSPAAA
                     PPPAAPAGAAAQVAEAQQLRERMMDLAARANAVKASMDGIERQQRASGLGMRADMAAA
                     AQRVEFLMGEANGALAARDNTAAKRNLDLAERDIEKLEAFLGR"
     gene            complement(5750..6115)
                     /locus_tag="VFA54_07910"
     CDS             complement(5750..6115)
                     /locus_tag="VFA54_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50769.1"
                     /translation="MRMLRLFWDAAVFLNPEVETLDEKHMLLCRAGELRNLWEQEGLE
                     NVEERPLEANLEFASFEDFWSPFLLGQGPAGAYVKRLAPEQVAALREELRRRLGPASQ
                     EPFPLPARAWAVRGTAGRQ"
     gene            complement(6073..6522)
                     /locus_tag="VFA54_07915"
     CDS             complement(6073..6522)
                     /locus_tag="VFA54_07915"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50770.1"
                     /translation="MFADAEAYGRFMGRWSRRMAPLLVDFAGVPDRGRILDVGSGTGS
                     LALTIAERKPHCRVEGIDVSREYVAYAAKRNRGDRVRFQVGDAQRPAPPSMRAFLFWS
                     LISSRIPAKPSTKCAASPAHGEALPPLFGITVIACGCCVCFGMRPYS"
     gene            complement(6762..7952)
                     /gene="alr"
                     /locus_tag="VFA54_07920"
     CDS             complement(6762..7952)
                     /gene="alr"
                     /locus_tag="VFA54_07920"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZS50771.1"
                     /translation="MERTFVEISRQKLIENYQAIQRLVGTSVEILAVVKADAYGHGAV
                     EVARALERAGARWFAVTSLPEAIELRRGGILGRVIVLADLARSDQDAFEEFGLTRMLH
                     SMDQLHDLEAWLQKQEGNLRSAATAGPDAFAPAGRSPQNGRRSLHLKLDSGMGRLGFH
                     EACLDEIACLLARRPDLHLEGLATHFASAEELTSGQTEEQIGRFQQFVSALAARGIRP
                     SFTHMANSAALVYWPETRASMVRPGLALYGYIMPARGGKAPAPPPLQPVLTWKAQVIS
                     VSDFPAGAPLGYNATYRTERPMRIGAVAAGYADGLDRRLSNGGPVLAAGRRVRIVGLV
                     SMDVCLVDLTSTPNVRAGDYITILGRDGSESLDAMQMAAYCGTIPYEVLCGIGKRVPR
                     LYVE"
     gene            complement(7958..9346)
                     /gene="dnaB"
                     /locus_tag="VFA54_07925"
     CDS             complement(7958..9346)
                     /gene="dnaB"
                     /locus_tag="VFA54_07925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZS50772.1"
                     /translation="MTSALGGIGFDRGLPANIEAEQFVLGSILLADAVFPQVAGTLDA
                     DDFVLEKHRKIFERMSDLHARSEKIDYVTLANELIKNNQLEACDGVAYLSALTEGLPR
                     LENIESYVRIVKDKALLRKLIYTSQHIMGQCLEAGEEVDEILSKAEELVLKVGDSRIR
                     GGLTSPRQIVERFPGGVNTFLDPSKRLRGLSTGFTKLDEMTTGLQPSELCVIAARPSM
                     GKTALALNIAQHVAVKLGRPAAIFSLEMSAESLLSRMLCALARVDSHRFRGGFLNMEE
                     RRRLSGALNQLVRSPLYIDDSASTNLMEMHAKCRRLKSERGDLALVVIDYLQLMSGKG
                     KFENRNQEISVISRGLKQLAKEIKAPVMALSQLSRAPEVRTGDHRPQLSDLRESGSIE
                     QDADLVGFIFREEVYKPDREDLHGLAELIIAKQRNGPTGKIDLAFLHKYTKFENRAED
                     LGDAEPPPESVQ"
     gene            9779..9856
                     /locus_tag="VFA54_07930"
     tRNA            9779..9856
                     /locus_tag="VFA54_07930"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9814..9816,aa:Pro,seq:cgg)
     gene            10006..10323
                     /locus_tag="VFA54_07935"
     CDS             10006..10323
                     /locus_tag="VFA54_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZS50773.1"
                     /translation="MSGIEIPIPNERLADFCRRWKISELRLFGSALRADFRPDSDLDL
                     LVSFASDADWSLLDHVAMEEELSGILGRKVDLVSRRAIERSSNWIRRKAILESAEPYF
                     VAR"
     gene            10310..10609
                     /locus_tag="VFA54_07940"
     CDS             10310..10609
                     /locus_tag="VFA54_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HZS50774.1"
                     /translation="MSRDEGNLLDILKAARLAIEFKGQTDKDEFLADLKTQSAVLHQL
                     LIIGEAVKRLSPEFRGAHPEVPWKLIAGTRDKLIHFYEGVDLDEVWKMVTADLPS"
     gene            10869..12047
                     /locus_tag="VFA54_07945"
     CDS             10869..12047
                     /locus_tag="VFA54_07945"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZS50775.1"
                     /translation="MMTRRKAVLALGAAFTGLAAEKDADRIRFCRVPNGGIQPQVALD
                     DSGTLHLVYYSGDTHHGDIFYARSKNGGASFSPALRVNAGGSAIAAGTIRGAQFALGK
                     SGRVHVAWNGSTHTGPVNPDSGTPGAPMLYTRLNDAGDEFEPERNLMLRSFGLDGGGS
                     IGADKNGNVYVTWHGIGQSEAKGAGKEGEARRRVWVTTSADSGKTFSGEAKAWAQETG
                     ACGCCGMKTFVDRKGDVYALYRSATESVHRDIYLLHSKDRGRTFQGKLLHKWNINACP
                     MSSMDFAENADTLVCAWETAGQVYWTRINGERTGDPAAAPGEGKGRKHPRLAVNERGE
                     ILLVWTEGTGWQKGGSFAYQVYDPAGRPSGETRLVPGIPTWSFAAAIGMPDGGFSILY
                     "
     gene            complement(12629..13672)
                     /locus_tag="VFA54_07950"
     CDS             complement(12629..13672)
                     /locus_tag="VFA54_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HZS50776.1"
                     /translation="MPHPPSKEELLSQPTRPFTIGADPSAASILERMEGTAFQGRQLG
                     QAFRVWKSMLRDRCSILMGMSGAMVPAGMRRLVVYMIQNRLIDCLVSTGANFFHDIHE
                     TLGYYHYQSSPAIDDVALAENLIDRMYDVLADEAKFREHDRWIGDFAARLDPSRPYTT
                     REFLYLLGRELAKTAKEDGIVSAAAKASVPLFCPAVGDSSIGIGVAVNRYHGNNRLMF
                     DVIGDVLEITRIVAESPETGVIYFGGGTPKNFVQQTEVVAIIMNTGKEGHKYAIQVVT
                     DAPHWGGLSGCTFEEAQSWGKISKDAQMVDCHCDSTIAMPILVSAMAQQVDLIRSRWK
                     PTFTMGRDLGIEI"
     gene            complement(13772..14320)
                     /locus_tag="VFA54_07955"
     CDS             complement(13772..14320)
                     /locus_tag="VFA54_07955"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HZS50777.1"
                     /translation="MLVAKKIFLTKGVGKHKERLTSFELALRDAGIAATNLVRVSSIF
                     PPRCKLISRKEGQKYLEPGQVVFAVMSENATREPHRLIAASIGLALPTDKGMYGYLSE
                     HHSFGETEETAGEYAEELAAEMLATTLNVEFDPDRSWDEKKEIYRISNKIVRTLNVTQ
                     SAIGDKRGWWTTVIAAGIAIFE"
     gene            14569..15744
                     /locus_tag="VFA54_07960"
     CDS             14569..15744
                     /locus_tag="VFA54_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HZS50778.1"
                     /translation="MGDGILPPVLGSTPRIQSLLIKPVSAVCNLDCEYCFYLDREADP
                     YKELPGRRMNEETLERLVSGFLFYSSPHSVFAWQGGEPTLAGAAFFERAVELQKQHGR
                     GGQSVTNALQTNATLLDDRWCSLFREYRFLLGVSLDGPEPLHDRYRFNKQRKATWKKV
                     MEGVALLQKNGVEFNILCVLSSANVERPAELYRFFRGLGIQHIQYIPLAEFDGLGLPR
                     PWTITPEQYGRFLCETFDLWWPDRKKIRIRFFDNIAEALAGIKPGNCTLHETCDSYCV
                     VEYNGDVYPCDFFVERNWKLGNINVDSFAEIARRQRRYAFAAKKTVPHPECLVCEFAQ
                     VCHSGCPKLRHGRNKRFEDLDYFCASYKAIYAKAIPKLKPEIAKILSRGVACGTGPA"
     gene            complement(15789..17222)
                     /locus_tag="VFA54_07965"
     CDS             complement(15789..17222)
                     /locus_tag="VFA54_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZS50779.1"
                     /translation="MLPAEAIQKLRKIAGDDAVLHQPQDLMLYEYDGSVEKSTPDCVV
                     LPRTTEQVAAIVKLAKEFELPLVGRGAGTGLSGGAIARSGGIMIGFARMNRILEIDLE
                     NERAVVQPGVVNLDLTLAVQGQGYFYAPDPSSQRACTIGGNVSENAGGPHTLAYGVTT
                     NHVLGLEIVLPDGTVVQTGGKEIDRAGYDLTGLLTGSEGTMVLVTKVIVRLMRKPELV
                     KTLLAIYDEPDDAAATVADITARQITPVAIEMLDGVMLRMVEEATHAGYPMDASAVLL
                     IELEGLREAVEAQTIDIEEVCRLNRAREVRVARSAEERDLLWKGRKNAFGAVGRVSPS
                     YYVQDGVVPRTRIADTLRFIYRVAAKYDLTISNIFHAGDGNMHPIILFNHHKPEEFER
                     TKQAGAEILEYCISVGGSITGEHGVGMEKNEMMPLLFPPETLEMIARLKSVFNPDGRL
                     NPGKLLPTGKGCLEVRPMASAGARMDW"
     gene            17260..19077
                     /gene="guaA"
                     /locus_tag="VFA54_07970"
     CDS             17260..19077
                     /gene="guaA"
                     /locus_tag="VFA54_07970"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZS50780.1"
                     /translation="MEKVVVLDSGGQYCHLIARKIRELGVYAEIRPLGTRAQRLSRYR
                     GVVISGGPASVFDPGSPQPDPGLFKLHLPVLGICYGQHLLAQHLGGKVEAGKRSDSAG
                     GGAREFGEAEIVIKSRKSIFAGLNGRERIWMSHGDYVSKLPPGFKVLGSTRDCRIAAM
                     GDDSRRLIGIQFHPEVTHTEHGLQILRNFLFRVCGCRGDWHPADQISDLEAAIRRHAG
                     GRRVLFFLSGGVDSTVAFTLAERALGPGRVHGVYVDTGFMRHGETQEIRGLFQQLRLD
                     GLEVIDARQSFFQALRGVTDPEEKRKIIGRLFVDIQDQVLSRPSYRSGQWMLGQGTIY
                     PDTIESGGSKHAAVIKTHHNRVDRIQQLIAEKRILEPLAHFYKDEVRQLGRALGVPDS
                     VLRRHPFPGPGLAIRCVCSPRPARPEPEPAIAELAEEAGYEAFLLPLNSVGVQGDSRT
                     YRKLTVLHGAPIHHEALARLTTRITNSFPQTNRVAAAVWPQNLIPGEWRIRRTSLTPA
                     RIRLLRQADHIVTRFLQEQFLYDLIWQCPVVLVPFSRNDGETIALRPISSVDGMTAEV
                     VHIPVPQLKQLAAELVESGGIDAVLLDISNKPPSTIEWE"
     gene            19109..19549
                     /locus_tag="VFA54_07975"
     CDS             19109..19549
                     /locus_tag="VFA54_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50781.1"
                     /translation="MAASHPTYDDVNLILRLFELRREEKMRQARDWFGKSFKARTLED
                     FQALCPPGSDPNAYYRMVVSYWDMAASFVTSGVLNEDLFYQSGRELLFVWERIRELIP
                     EMRKTFKNPTLLKNMETVANSFIERMNREAPEAYPAFSAMVRGN"
     gene            complement(19985..20887)
                     /locus_tag="VFA54_07980"
     CDS             complement(19985..20887)
                     /locus_tag="VFA54_07980"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS50782.1"
                     /translation="MAKLGFLGLGLMGYPMARNLLRAGHEVALWSHTEAKARKLAQEE
                     KGTFCAKPRDVGAAADYIFACVGDSAMSENVMVGPDGVIQGAKPGTVVADCSTIAPTA
                     SRRIGQTFAAKGIHFLDAPCTGSTSGAQGGTLTFMIGGSKEVFERAREYFLIMGKNLF
                     YCGGAGMGLHAKLSQNLVLSNVMQGFIEGMVLATKAGVDPELMFQILDNSAAKAGLIS
                     AKAPFIFRRDFTTNFSLRWMHKDIGLMLETGRELNVPLPSTALVHELFGASIARGHAD
                     EDFCSAVTLLEDWAGVQVKAKKNT"
     gene            20969..21946
                     /locus_tag="VFA54_07985"
     CDS             20969..21946
                     /locus_tag="VFA54_07985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HZS50783.1"
                     /translation="MKSRVLVSKRVYPPAIEYLRQRVELEYNDSNESYSPHKLIEKLK
                     GKQGVVCQVTDKFTPGVMDQLPDLKIISNIAVGVDNIDLRAATARKILVTNTPDVLTD
                     TTADFAFALLLAAARRVVEGDAFIRSGQYKQWLMDLLVGHDVHHRTLGLVGMGRIGQA
                     MARRARGFSMRILYYDSSRRPAELERELGLEYTPFDQLLSESDFVSLHVPLAPETRHM
                     IGGPQFAIMKRTAILINTARGPVVDEAALAAALAGGRIAGAGLDVYEEEPKLHPGLQR
                     LKNVVLAPHIASGSHDTRIRMCMLAAENLVAGLSGKLPPNLVNRELMPA"
     gene            21943..23241
                     /locus_tag="VFA54_07990"
     CDS             21943..23241
                     /locus_tag="VFA54_07990"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HZS50784.1"
                     /translation="MKDRLRRLFLETLDRLSLERIIPHRVSVRDGVMEIGGDRIELRA
                     YKKIIIVAIGKAAFQMARSVKALLEPAVASGVVASSEPAEVPLPFLLHYRGGHPYPNA
                     ESLRAAEVILELLRQLNQDHLVIYLLSGGGSAVVEKPVDPSISLEDMRAFYRVLVTCG
                     ANIVEMNTLRKHFSATKGGRMAYAAHPARQVTLYVSDVPVDRPSAVASGPTMPDESTV
                     ADCWEIVKRLDLLPRFPASIRRMFEAGTIPETPKPGSSRFKRSTWHALLTTEQGTEQL
                     AAAAREQGWIVDQDLSVDDWPLIPAAEHLLERLLRLRAENPRRTVAIVSGGELSSPVT
                     GDGVGGRNQAFVLACAQRIAKKNIAVLSAGTDGIDGNSPASGAVADGETLDRAAQLGL
                     SPEDYFQRSDSFGFFSRLDDLVVTGPTGNNIRDLRLLVAW"
     gene            complement(23242..23451)
                     /locus_tag="VFA54_07995"
     CDS             complement(23242..23451)
                     /locus_tag="VFA54_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50785.1"
                     /translation="MNIRLQPGKTEYTETEAARALGVSPDQFRSLLLKHVLEKEEALG
                     NVGAMRFRPSDLLLLGMLGRGQAIE"
     gene            complement(23610..23858)
                     /locus_tag="VFA54_08000"
     CDS             complement(23610..23858)
                     /locus_tag="VFA54_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50786.1"
                     /translation="MNIRLEPGKSEYTETEAARALGISQAQFRSLLLRHVVEEEESLV
                     NVAIMRFRPSDLLLLSMLVEGRLAHKQSPSAGHKPPPG"
     gene            complement(24096..25088)
                     /locus_tag="VFA54_08005"
     CDS             complement(24096..25088)
                     /locus_tag="VFA54_08005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZS50787.1"
                     /translation="MPRAFLRTWLIVDALLALGAAVYAASRHLPGAMVVPIVAAFLMQ
                     ISFYLALGFPEMRGWLARRFSPPRLAAIAVVWAITPYLIYSLPAGVFQFSALAKLGGL
                     AGTLGFIFVVFPVKGSRLTWPDVLAAGTIAAALLSRVLRQVYLSPIPGLRLESMGHVM
                     IIGLGAMAFLCLRQVEGTGFRFQTSREEWKTGIKQLLLFLIPGAPLALLTGAAHFQPL
                     RTEPWLYPLQAAGTFLGMYAVVALSEELFFRGVLQNLSAANLGGATAAQAATSIIFGL
                     CHLPFREFPNWRFAALGALLGWFCGQAYRETRSVVASSLTHALAISAWRLLFSA"
     gene            25076..25849
                     /locus_tag="VFA54_08010"
     CDS             25076..25849
                     /locus_tag="VFA54_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50788.1"
                     /translation="MRGASHHLNQVCRAALWAAACALAVSSAVSPLHGQEPEESLIRE
                     IAAKASQAEAARAHYTYRQTMIFQELDAHGAEVGRYQEVRDVIFSPGQKRSEVLVGRP
                     HRDLKRLKLTDEDFRDVREVNPFVLTRDELFYYQSKFQGREAMDGVACMVYRISPRQI
                     LDGQRLFDGLIWVSEKDRQIVRAEGRPVPQIYRSTGENLFPRFTTIYRPVDGQHWFPV
                     RTLADDTLYFRSGPQRVRITIRYDEYKRFSSDTTIEYEK"
     gene            complement(25867..26703)
                     /locus_tag="VFA54_08015"
     CDS             complement(25867..26703)
                     /locus_tag="VFA54_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50789.1"
                     /translation="MRRLTAHSRRDEVAFDNRDRLAPVQSFQREQGVTSPKGTISFSP
                     EGAAWLPRIALSYGQSFHVDDPRIGTTAIAGGTVVSKARAYQLVIRKTAAHTEFKVTL
                     AHVTTAQQLARIDNDTGLQEDVGPALIRSMTVSARRDFGLGSLQVLFAKADARDRLTG
                     EPTPEAPRLIGDVLGTVERLPFKLRARGEYEYVGRKPLGDGFAAVPVQEFRGAMIRPF
                     DSKGIDVGINLFLVRGFAGQTLETLALPGEAEPFERISGFRLKSYATVSVTYRFRRAS
                     QL"
     gene            complement(26669..28129)
                     /locus_tag="VFA54_08020"
     CDS             complement(26669..28129)
                     /locus_tag="VFA54_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS50790.1"
                     /translation="MTPERFPLAPDVRRALPPSLFLAAKQAALLVLFSGWACASSGVA
                     GQVTDPQGKAVAGAEVTLVRRSDVFQMENKTDGQGRFLFPSVEAGEYRLTAEAAGFPS
                     VSRDIRVSGNALLEADLHFSQLAVQSQSVTVTEEVEGPGIFSPDPASRVLIRQEILDA
                     NPGRPGMPLSIPGMPVESPAGGIKPPQYFVPGVAGDHGEPIGQFFQVGGYLFPNNLPA
                     NAHGNGYADPNVLIPIAIESVQTDGGSFNVREGNNSMNASMIFGLRDRLHPMVRFTAD
                     AHDVNLVAGWSPASAETKAWAGLELSLGNGFLGRPERRKQYKLNGSRVFQFGRHDLTV
                     YGIGYHGFSYLPGMAPIGVPVPGDTIDPRQREEASSGIAIVNDVWRLTATRQLQFSGY
                     FRNYYLDVRPDFGGGLIRQSENRTATSQSVNYAYRIARFFSLLLGADYRREAPRNLDL
                     DKFNEAAAAFYPLTSNDITIQFYTPYAALDGALTPR"
     gene            28362..>28832
                     /locus_tag="VFA54_08025"
     CDS             28362..>28832
                     /locus_tag="VFA54_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50791.1"
                     /translation="MINKRTLVAMGAGLVVTAVLVAAIVFIQRGSTPRLVGEISRVRT
                     LEMDPSSSVAIADFRFTNDSHYAFIIATIDLSVIDFKGETRLGQVISASDANRLFQLF
                     PALGPKTAEPLIIKTKVAPGASVTGMVAARFELSKTDLDARRKIILTITDVDGPV"
BASE COUNT         5591 a         8825 c         8782 g         5634 t
ORIGIN      
        1 tgccaaaaaa tcatccggct gctagccctc agcctggttg cttgcgccgc cgggaatgcg
       61 caaactcctc ctgtgttcga cggccaggca gccctggacg ccacgcggcg cgtggtttca
      121 ttcggcccgc gccgtcttgg atccgccgcg cacgaactgg ttcgccagtg gctcaaaacc
      181 gaactgcggc ggctccggtg cgaagtggcg gaggacgctt ttacagcccg gaccgtgctt
      241 ggcccccgcc cgatgacgaa cctgatcgcc aagttcccgg gatcctcgcg ccgcatcgtg
      301 gtgttgagtg gacattacga cacctacgac cggcctggtc tccgcttcct gggcgccaac
      361 gacggcggtt cctcgacggg cattcttctt cagctcgcgc gctccgtcgc ccggattgcg
      421 caccgcgata cgatctggat cgtgtggctg gatggagaag agtcgctggt gaactggtcc
      481 ggcgacgatc acacctatgg cagcgcgcat ctggcccgcc gctggcgctc ggacggcact
      541 gccggctcga ttctggcgct gatcaacctg gatatgatcg gcgacgccga tcttgatctg
      601 gcttacgaac tgaactcgac aggctggctg cgtgatctgg tgtggtccgt ggcgtggcgc
      661 ctgggctact ctacccaatt tcccagggac cggcccaacg ccatcgccga cgatcatgtc
      721 ccgtttgccg agagcggcat cgcggcgttg gatttgattg acctcgacta tgggccggac
      781 aacgcgtact ggcacacggt gaatgacacg atggataagc tgagcgcgcg cagtttcggg
      841 attgtcggcc aggtcctgct cgaaaccctt cgcgttctcg agcagcgcgg caacggcggt
      901 tgaggacggt ttgcgcggct tgaggtttct ggcggcctct gagctcaacg ttcggccgag
      961 ttgtacactg aggggcggca ggccgctccg gtccggctga tcagaagccg gacgctgcga
     1021 cagggagatg ggcaatatga agatcacgcg acgttcattt tttcgctggg ccggcggggc
     1081 cgccggcatg gctgcgtcca gcgtccccgc gcgggccgat gcgccggcgg acattcgcat
     1141 cacggatgtc cggccgctga gcatcaacgg acggaccttg ttcgtgctag tgcgcgcctc
     1201 caacggcatg ttcggtctgg gcgaatgctc tcccatgaat ctgccggtgc tcgccgccaa
     1261 catccagaac gttctcaagc cgcgcctgct gaacaaagac ccttttcaag tcgaacccct
     1321 ctacgacgag ctgctctatc cgaatttcaa gctcgggccg atgggcgcag tgtcggaggc
     1381 gatcgccggc gtcgatatgg ccctctggga cctgaaagga aaaattctcg gcgttcccgt
     1441 ctacaagctg ctgggaggca agtaccgcga ccgccaacct gtttacttca gttacggccg
     1501 cggcagcgcc aaacgcgaca ctccggaaag cgtggccaag ttgatggcgc aggcggccga
     1561 gcgcggcttc accggggtga agattcgcat ggatttcggc agcgccgttt tggacgcgcc
     1621 cgatgacccc tccgaggcgt tcatctccgc cattcgccgg gccgtaggcg actacgtgga
     1681 tgtcgctttc gacgccaaca acggctacac agccaaaagg gctatcgctg tcggcaggcg
     1741 cctttacgaa aggtacaata tcgcgtggtt cgaagagccg acgccgcagt acgactatgc
     1801 ggcgatggcg caggtggcgg cggcgctcga agtgccgatc agcgccggcg agcacgaata
     1861 cacgatctgg caatttaagg atctgattgc gcagagcggc atctcgatcg tgcagccgga
     1921 tgtcgccaaa tgcggcggcc tcacccaggc aaagaagatt gcggcgctcg ctttggccca
     1981 taacaagcat atcgtcgtac acaacacgac tcccacggtc gccacggcgg ccgcgctgca
     2041 ctttgctgcg tcctgttcca atgccgctcg ccgccaggag tatccgggag agcgggccga
     2101 cctcgaccgc ttttttgaaa acaagctgga gttcgatcgc gggctcctga agatcccgga
     2161 actgccgggc ctgggcttgg tggtaaggca gagcgaactg gagaaattgt gacgccgcgg
     2221 caaccggacg gccgctcgga tctggaccgg atgccggcgt tggacggccg ccgctcatgc
     2281 aacgattcgc gccgccgtga cggagagcat gtcggtatga cctcctgtcc gacctgcggc
     2341 gcaattctgc aaggggccgc cggctgcccc acttgcgggc cggtgcgaac cggtttcgag
     2401 cgttggacgg cggctctggc gtacttttgg attaccggca ttgttttttt attcgccgcc
     2461 aggcgccgcg ccaaccccta tgtccggttc cattgtttac aagccgtctt ttacggtgcg
     2521 gtggtcttgc tgatgaatgc cgtactggcc atagtggcgg tccaaatcgg gaccatggga
     2581 gcgttcccga tcatatacgc cacggtctgg ggagtgatac ggatcgggtt gttcctgctt
     2641 tggattctag gcatgctcaa ggcatatgag gggatcgaat acaagctgcc ttgggtaggg
     2701 aatcttgccc ggaaatacgt ccccgcccgg agggcgtagc accccggtag gactattcat
     2761 tcgcccataa ggccgatata atacgcggag cggagggcgc catgcttcga tttgcggcca
     2821 ttctattggc tctcatgacc ctgactggag acttgagcgc gcagcagaag aagcgcgtgg
     2881 cggtgctcaa cttcgattac gcgaccgtgc atagcagcgt cgcctcgatt ttcggcagcg
     2941 atgtggacgt cggcaaaggg gtggccgatc tgcttgtgga cgatctggtg aaaagcggtg
     3001 tgtactcggt aatcgagcgc aaggcgcttg acaaaattct ggccgagcaa aacttctcca
     3061 acagcgaccg cgccaatccg cagacagcgg ccaaactagg ccagttgctc ggcgtggacg
     3121 cgattgtgat cggcagcatc acgcagttcg gccgggatga caaggccacc aacgtcggcg
     3181 gaggcgttct cggcgggttc ggcagcaagt acgggatcgg cggcgtatcc aaacgccaga
     3241 gcaaggccgc agttgggatc acggcccgct tggtgagcgt ggagacaggc gagatcctgg
     3301 gcgtcgccga gggaaaaggc gagtccacac gctccggagc gggcctgctg ggcgcgggag
     3361 gcgccggcgg cacggcggct ggcggcggct atgatatgac cagcaagaat ttcgccggta
     3421 cgattctggg cgaggcggtg aaccaggcgg ttgcgatgct ggcgcgaaat ctggaccaga
     3481 acgcggcccg gctgccctcc cggaagatcg ccgttgacgg tttggtggcc gatgtttccg
     3541 gcagcacgct gacgttgaac gtgggaagca aggcgggcgt gaaagtgggg gaccggctgc
     3601 aaatacgccg gcccgtgcgc gagatcaagg acccggccac aggcaaggtt ctgcgaaggg
     3661 tggaagacaa gctcggcgaa atcaccatca gcgaggttga cgagaactca tcggtcggaa
     3721 cgtacgcagg cgcggcgccc aaagtgggtg acagggtggc ggcgttgccg taacgagaag
     3781 cggcgatgta tttccaaccc ggcgatacga tcggcgacta cgagatcctt agcgttctcg
     3841 gcgaaggcgg catggggaag gtctacaagg tccgcaatgt aatctcggac cgcctggaag
     3901 ccatgaaagt gctgctcccc gacctgcatg gcgacccgga actggccgac cggtttgtgc
     3961 gggaaatcaa ggtgcaggcc agcctcgatc atcccaacat cgcggctctt cgcactgctc
     4021 tgcgcgcgga aaatcagctc atcatggtga tcgagttcgt cgagggccgc acgctggtgg
     4081 acttgctcga gcggggcccc atccctctgt atgacggcat cgagtacatg tgccaggcgc
     4141 tttgcgccct cgcgtatgcg cacgagcgcg gggtgattca tcgcgacatt aagccggcga
     4201 acatcatgct ggcgaacgac ggcgtgatca agctcatgga ttttggcatc gccaaagccg
     4261 ccgctgaccg caagctcact atgacgggca cgactatggg ttcgctctac tacatgtcgc
     4321 cggaacaggt gaaaggaacg acggcgcttg acgcgcgctc cgatctgtat tcgctgggga
     4381 tcactcttta cgaggtcgtt accgggacgc gcccgtttca gggcgacagc gattacgcga
     4441 ttatgtcggc gcatctggag aagacccccg taccgcccgt cgaggtggac ccgaacctgc
     4501 ccggagcgtt gaacgagatt attttgaaat cgattgccaa agatccggcg gaccgttttc
     4561 aaaatgcgaa tgcgttccga accgcgctga agagcgtgca agcggagctt attcccgagg
     4621 ctgcggcctt tcccggagcg cagcggcaga ctccgcgcac agaggccgcc ccgagcccgc
     4681 gcctcgatac agcgccgccg gttccgccgc ccatgccttc cgaagcgatc cccgccgctc
     4741 cgcgcggatc gccgccagtg gctcgaagat ccgccccaat cccgcctgct cccagccgcc
     4801 ggggtttcta tatgctggcg ggcggcgtgc tgacgctggt gctgctgatc gccgccggca
     4861 tagaggtggc caactggcgg aagaccagag cgggcgcgat tcccgaaaca gccccggccg
     4921 gcgctgatga gcaaaacact cggcaaccag taaattcaac gggcggcagc cagccggaag
     4981 cgtcaccgct cggcgctgcg cgcagcactg atgcggcagg ccccgcccag cccaatacaa
     5041 caccggcggg cttggcgggg cagagcggcg cggccgcagt cgccggcgcg gccgccacgg
     5101 attctggaag ccggaaagcg ggagcgggtc aagatgcggc attctccgct gctcctaccc
     5161 cggcgcccca ggccggcaga gttgcgcgcg agccgggccg tacggggcaa gcagcgctgc
     5221 gcagcgtgcc ccagcaagcc caacaacctg cgaatcgcac acccaaggcg gccgcacccg
     5281 atctggtttt gccttcgccc gccgccgccc cgccccctgc ggcccccgct ggcgcagcgg
     5341 cgcaagtggc ggaagcgcag caattgcgcg agcgcatgat ggatctggcc gctcgggcga
     5401 atgccgtgaa ggccagcatg gatggcatcg agcggcagca gcgcgcttcc gggttgggca
     5461 tgcgggccga catggccgcg gcagcccaac gcgtcgaatt tctcatgggc gaagccaatg
     5521 gcgctctcgc cgcccgcgac aacaccgcgg caaaacgcaa cttggacctg gccgagcgtg
     5581 atatcgagaa actggaagcc ttcctcgggc gctaaaagat tttgaggaac agcctcgttt
     5641 gcgggcttaa ggatgcgcga attggagtct tcgacgcttc cagcaaaggg aggcgaacgc
     5701 cgcccaacaa cccaggagat ctgcgcttgg tccccgcggc aaagcgctcc tattggcggc
     5761 cggccgttcc ccgcacggcc catgcgcgcg ccggcaatgg aaacggctcc tggctcgctg
     5821 ggccgagacg gcgtctcagt tcctcgcgca aggctgcgac ttgctccggg gcgaggcgtt
     5881 tcacgtaggc gccggcagga ccttggccca gaagaaacgg gctccagaag tcctcgaaag
     5941 atgcaaactc caggttcgct tcgagcggtc gttcctcgac gttctccagg ccttcctgct
     6001 cccacagatt ccgaagttcg ccggcgcggc aaagcagcat gtgcttctca tcaagcgtct
     6061 cgacttccgg attcaggaat acggccgcat cccaaaacag acgcagcatc cgcatgcgat
     6121 caccgtaatc ccaaacagcg gcggcaatgc ttccccgtgc gcgggtgacg cggcgcattt
     6181 cgtcgagggc tttgcgggga ttcgggatga aattaaggac cagaagagaa aggctcgcat
     6241 cgaaggcggc gcggggcgct gcgcatcccc gacttgaaaa cgcaccctgt ctccacggtt
     6301 tcgctttgcc gcataggcta cgtattcccg ggagacatct atgccttcga cccggcaatg
     6361 cggctttcgt tcggcgatcg tcagagccag cgatccggtc cccgaaccca cgtccaaaat
     6421 gcggccccga tcgggaactc cggcgaaatc caccagcagc ggcgccatcc ggcggctcca
     6481 acggcccatg aaacgcccgt aggcttctgc gtccgcgaac atttcaccac ccggctgctg
     6541 agccgggaga agggagccgc cggccaggat cagaaaagct ctccgtgacc acatccggct
     6601 cgaattcagc gctcctgctc cgaagccaag aagtgaatgt taaccccatt ttaccgggga
     6661 agtcggctcg ccgagagagg cgggcccgcg ctggtaagat tttgcttggc gccggcatgc
     6721 tgttgcgcgg gcaacccttg cgtccctgcc gcaccgggcc ttcactccac gtagaggcgg
     6781 ggaactcgct tcccaatccc gcaaaggact tcgtagggga tggttccgca gtacgcggcc
     6841 atttgcatgg catcaaggct ttcactgccg tctcgcccca agatggtaat gtagtcgccg
     6901 gcccgtacat tgggagtgga ggtgagatcg acgaggcaca catccatgct caccagcccg
     6961 acgatgcgca cacgcctgcc ggcggcgagc accggcccgc cattggaaag ccgccggtcc
     7021 agcccatcgg cgtagccggc tgcgactgcg ccgattctca tcggcctttc cgtgcgatac
     7081 gtggcgttgt agccgagcgg cgctccggct ggaaaatcgc tgaccgaaat cacctgcgcc
     7141 ttccaggtca gaacaggctg caagggcggc ggtgcgggcg cttttccgcc ccgggccggc
     7201 atgatgtaac cgtaaagcgc taggcccggc cgcaccatag aagcccgcgt ctccggccaa
     7261 tagaccagcg cggcgctatt cgccatatgg gtgaaagaag ggcggatgcc gcgggcggcc
     7321 agagcgctca cgaactgctg gaaccggcca atctgctctt cggtttgtcc gctcgtgagt
     7381 tcttcggccg aggcaaagtg cgtggccaga ccttccaggt ggaggtccgg gcgccgggcc
     7441 agcaagcaag caatctcatc gagacacgcc tcgtggaacc caagtctccc catgccagaa
     7501 tcaagcttca aatgcaggga ccggcggcca ttctgtggcg atctgcctgc tggcgcgaag
     7561 gcgtcaggac ctgctgttgc tgcggagcgg aggttgccct cctgtttttg cagccaagct
     7621 tcgagatcgt gaagctggtc catgctgtgg agcatccggg tgaggccaaa ctcttcgaag
     7681 gcgtcctgat cgctgcgcgc cagatcagca agaacgatca ctcggcccag aatgcctccg
     7741 cgccgcagtt cgattgcttc gggaagagac gtgacggcga accagcgcgc tccggcccgc
     7801 tccagcgccc gcgccacctc gaccgcgccg tggccgtacg cgtcggcctt tactacagcc
     7861 aggatttcga cagaggttcc taccagccgc tggattgctt gataattttc aatcagcttt
     7921 tgccgggaaa tttcgacgaa cgtccgttcc accggtttta ttgtaccgat tcagggggcg
     7981 gctcggcgtc tcccaaatct tcggcccggt tctcgaattt cgtgtatttg tgcaggaacg
     8041 ccagatcgat cttcccggtc gggccgttgc gctgcttggc gatgatcagc tcggcaaggc
     8101 cgtgcaggtc ttcccgatcc ggcttataaa cctcttcgcg aaagatgaag ccaaccaggt
     8161 ccgcgtcttg ctcgatcgat ccggactcgc gcaggtcgct caattgcggt cggtgatcgc
     8221 ccgtgcgaac ctccggcgcg cggctaagct gggaaagcgc catcacgggc gccttgatct
     8281 cttttgcgag ctgcttcaag cctcgtgaga tcaccgaaat ttcctgattg cggttctcga
     8341 actttccctt gccgctcatc aactgcaagt aatcaatcac caccagcgcg agatcgcctc
     8401 tctcgctttt caaccgccgg cacttggcgt gcatctccat caggttcgtg ctggccgagt
     8461 cgtcaatata caaggggctg cggaccagtt gattcagcgc tccggaaaga cgccggcgct
     8521 cttccatgtt caaaaatccg ccgcggaaac gatggctgtc cacgcgggcc agcgcgcaca
     8581 acatgcgcga cagcagcgac tccgccgaca tctccagcga gaaaatcgcc gctggcctgc
     8641 cgagcttgac ggctacgtgc tgagcgatat tgagggccag agccgttttg cccatagagg
     8701 gccgcgcggc gatgacgcag agctccgagg gctggaggcc tgtggtcatc tcatccagct
     8761 ttgtgaaacc ggtgctgagg ccgcgcaagc gcttgctggg atcgagaaat gtgttgacgc
     8821 cgccggggaa tcgctccacg atctgccggg ggctggtcaa acctccgcgg atacgcgaat
     8881 cgccgacctt gaggaccagt tcctccgcct tgctgaggat ctcatctact tcctctccgg
     8941 cctccaggca ctgccccatg atgtgctgcg aggtatagat gagcttccgg agcagagcct
     9001 tgtccttgac gatgcggacg tagctctcta tattttccag acgcggcagg ccttcggtca
     9061 gcgcggagag ataagcgacg ccgtcgcagg cttcgagttg gttgtttttg atcagctcgt
     9121 tggccagagt gacgtagtcg attttttccg aacgcgcatg aagatccgac atgcgctcga
     9181 aaattttccg gtgcttttcg aggacgaaat cgtccgcgtc gagcgtgccg gcgacttgag
     9241 ggaacaccgc gtccgccagc aagatcgagc cgagcacgaa ttgctccgct tcaatattgg
     9301 cggggagacc tcgatcaaaa ccaattccac cgagagcgga agtcatgagc aggggcgacg
     9361 acggtcgaga gtaaacgatc ctgcggcttg cgtgacaaga aaatgtttca tgtttgtttc
     9421 gaaccggtta cattgtgaat cacgccgaaa catccacaat tggataggta gagatcttta
     9481 gcctttttct ttcagggagt tacgctgtgg aaccgcatgt gcataaccgg ccctggcggc
     9541 gcggcgttgc atggaaaaca agcccgtttg attgcagcac attagagcgt aagttgccgt
     9601 acgaaacatc accaattcac atcgaacttg tttgtaacaa tgtagcaggt ttctgtggcg
     9661 aacaaaagag aaatctgggg agaagaggcg gggaaactga tccgcacggg aggagccatt
     9721 gtgcggcatt gacacttccc gcaccaaccg atacactaaa atgacctttc aagacgatcg
     9781 gggcgtggct cagcctggct agagcgcctg gttcgggacc aggaggtcgg tggttcgaat
     9841 ccactcgccc cgaccattta tctcaaaaac actcatcacg atacgccgac ccgggttttg
     9901 cgcccgtgat cggcgttttt gctggtgtga ccagatttgt gcccaccctt tgctgcccat
     9961 gccaggacgg ccgccttggt atgatgccgg tagggcgggg acgccatgag cggaatagag
    10021 atcccaatcc cgaacgagcg ccttgcggac ttctgccgcc gctggaaaat ctcggaactg
    10081 cggctgtttg gctcagcctt gcgagcggac ttccggcctg atagcgacct ggacctgctt
    10141 gtgagcttcg cttcggacgc cgactggagt cttctggatc acgtggcgat ggaagaagag
    10201 ctctccggca tcctcggccg aaaggtggac ctcgtctctc gccgggcgat tgagcgaagc
    10261 tccaactgga tccggcgcaa ggccattttg gagagcgcgg agccctactt tgtcgcgcga
    10321 tgaaggcaac cttctggaca tcctgaaagc tgcccgtttg gccatcgagt tcaaaggcca
    10381 aacggacaag gacgagtttc tcgccgactt gaagacccag tccgcggtct tgcatcagct
    10441 cttgatcatc ggcgaagccg tgaagcggct gtctcccgag ttccgtgggg ctcatccgga
    10501 agtaccgtgg aaactgatcg ccggaacgcg cgacaaactg attcactttt atgaaggtgt
    10561 ggacctcgat gaggtttgga aaatggtgac ggcggatctg cccagttaac tcagtgaatt
    10621 gagccactgg ttccccgtgg tttctaaagg agcacgcgct gttagccctc cctccacttg
    10681 cgaccgggcg cgctcaaagg cagcgcaaac cgcgtccgct tgcggatgca catacctccg
    10741 cttcgtagtt ccgaaacaac tatggcgggc taactgcgcc agtggcctgg atgtgggcgg
    10801 accacccgtg aggcatgtgc ggctgctcgc ctctccgctg ctgggataca atgggcagaa
    10861 acgacaccat gatgacgaga agaaaggccg tacttgctct tggcgccgca ttcaccggtt
    10921 tggcggctga aaaggacgct gatcgaatcc ggttttgtcg cgttcccaac ggcggcatcc
    10981 agccgcaggt ggcgctcgac gacagcggca cgctccacct tgtttattac tccggcgata
    11041 cccatcacgg agacatattc tatgcgcgtt cgaagaatgg cggcgcgagc ttttcacctg
    11101 ctctgagagt caatgcaggt ggctccgcca tcgcagcggg cacgatccgc ggagcccagt
    11161 tcgcgctcgg taaatcaggc cgcgtgcacg tcgcatggaa cggctccacc cacaccggcc
    11221 ctgtcaatcc ggattccgga acgccgggcg cacccatgct ctacacgcgc ttaaatgatg
    11281 cgggagatga gttcgagcca gagcggaacc tcatgctccg ctcgtttggc ctcgacggcg
    11341 gcggctcaat aggcgccgac aagaatggaa acgtctacgt cacttggcac gggattggac
    11401 aatcggaagc gaaaggcgcc ggcaaagagg gagaggccag acggcgcgtt tgggtgacga
    11461 cgtcagcgga tagcgggaag acgttctctg gagaggcgaa agcttgggcg caggagacgg
    11521 gcgcgtgcgg atgctgtgga atgaaaactt tcgtcgatcg aaagggtgat gtatacgcac
    11581 tctaccgctc ggcaacggag tccgtgcacc gcgatattta ccttttgcat tcaaaggatc
    11641 gtggcaggac ctttcaaggc aagctgctcc acaaatggaa tatcaacgct tgtccgatga
    11701 gcagcatgga ttttgccgag aacgcagata cgctcgtctg tgcctgggag accgcgggcc
    11761 aggtctactg gacccgcatc aacggagaaa ggaccggcga tccggccgcc gccccagggg
    11821 agggaaaggg ccgcaaacac cctcgtctgg ctgtgaatga aaggggcgaa atcctactcg
    11881 tttggaccga aggcacgggt tggcagaagg gcggctcatt cgcgtatcaa gtttacgacc
    11941 ccgcaggcag gcccagcggc gaaaccagac tggttcccgg tattccgacc tggagttttg
    12001 ccgcagccat aggtatgccg gacggcgggt tttccatcct atattgacac tgctattcga
    12061 aactgcgatt agcttcattt ctccggggcc gctgagggct gcggttcgca atttccaacc
    12121 aatcggggag gtagcctggc agtggaacct cgattgctca cgttcccgcg cggacagtca
    12181 ccgtatttcg aatgtcccac ggtagaactc gcgatcacca cgtcggcccc ggacgctagg
    12241 atgagcgcac atccgcctgg cgactatgcc gtgaaccttt ggctgataca gcattgcgtt
    12301 cgacagcaca agagttttcc gtcttgattt gagcggctcg atgatgactt ccacggcgtt
    12361 ctcggcgcct cgcaggtcat tgataacgga atcttccggc gcgtgattgc cgcgattgac
    12421 cgggcgtgcc catcttcccg ccgcgatcct gcgcgcccaa ctgcgaccgg ttttgcgctc
    12481 gccccatgct ctcggcgttg ctgcctttgg ctcgcactgc ccgagaaagc agcccagcct
    12541 ttgtttcccc ggcagacgcg agcaagggcg agcaagatga agggctgatc cctgatagct
    12601 gacggctgat cgctgccatt tccctttttc agatttcaat gcccagatcc cggcccatcg
    12661 taaatgtcgg cttccaccgg ctgcgaatca aatctacctg ctgtgccatc gcggagacca
    12721 ggatgggcat ggcaatcgtg gaatcgcagt ggcagtcgac catctgcgca tctttggaga
    12781 tcttgcccca gctttgcgct tcttcgaacg tgcagccgga aaggccgccc cagtgggggg
    12841 cgtcggtcac gacctgaatg gcgtatttgt ggccctcttt accggtattc atgatgatgg
    12901 ccaccacctc ggtctgctgc acgaaattct tgggtgtacc gccgccgaag taaatcactc
    12961 cggtttccgg cgattcggcc acgatgcggg taatctccag cacgtccccg atcacgtcaa
    13021 acatcaggcg gttgttgccg tggtagcggt tgacagctac cccaatgccg atcgagctgt
    13081 ctccgacagc cgggcaaaac agcggtacgc tggccttagc cgccgctgag acaatgccgt
    13141 cctctttggc cgtcttggcc agctcgcggc ccagcaggta aaggaactct cgcgtggtgt
    13201 acggacgcga cgggtccagc cgcgcggcga agtcgccgat ccaccggtcg tgctcgcgga
    13261 atttcgcctc gtcggccagc acgtcgtaca tgcggtcaat caggttctcg gccagggcca
    13321 catcgtcgat cgccggagag ctttggtagt ggtaataccc taacgtctca tggatgtcgt
    13381 gaaagaagtt cgcgccggtg gaaactaggc aatcaatcag ccggttctgg atcatgtaga
    13441 cgaccaggcg ccgcatgccc gccggcacca tggcgccgga catgcccatt aggatcgagc
    13501 agcggtcccg aagcatactt ttccacacgc ggaaggcctg gcctaattgc cggccttgga
    13561 aagcggtccc ctccattcgc tcgagtattg aggcggcgga gggatcggct ccgatggtga
    13621 agggccgcgt gggctgagat agcagttctt ccttgctggg aggatgtggc atattggaaa
    13681 accctctggg gaatacgatt tacaccggcc ggaagacccc gtttcacctg ccggccgcgg
    13741 cgcccctttg gccaggcgtc tgattaacaa tttattcgaa aatggcgatg ccggcggcga
    13801 tgaccgtggt ccaccatccg cgcttgtcgc ctatcgccga ctgggtgacg ttaagcgtgc
    13861 ggacgatttt gttggaaatg cggtaaatct ctttcttctc gtcccaactg cggtcgggat
    13921 caaactctac attcagcgtg gtggccagca tttcggcggc gagctcctcg gcgtattccc
    13981 cggccgtttc ttcggtttca ccgaaggaat ggtgctcgga aaggtagccg tacattcctt
    14041 tgtcggtggg cagagcgagg ccgatgctgg cggcgatcag ccggtgcggc tcccgggtgg
    14101 cgttttcgct catcaccgca aacacaacct gccccggctc cagatacttt tggccctcct
    14161 tgcgggaaat gagtttgcag cggggaggaa agatgctgga aacccggacg aggttggtag
    14221 ccgctatgcc ggcgtcccgc agcgccagct cgaaactcgt gagccgctct ttgtgcttgc
    14281 ccactccttt tgtcagaaag atcttcttcg cgaccagcat tgatcccagg gagtcctcct
    14341 tcacggagtc agattcggaa tttttcaagc taacagaaat gggcaggagg gtcaacgaat
    14401 ttcacggacg accatttcgc ggcggccgcg cggccggctg gcgccagggc gcgctgcccg
    14461 cccccgcagc gcagggcgat tgttttggcg ggcccgagtg gcattcacgc cgccgtgacg
    14521 gcccggctcc ggaataggac gcccggcgct gcgtgctaca ctcgggccat gggcgatggg
    14581 attttgcccc cggttctagg ttccacgcct cgtatccaat ccttgctgat caagccggtt
    14641 tcggcggttt gcaatctgga ttgcgagtat tgcttttatc tggaccggga agccgacccc
    14701 tacaaagagc tgccgggccg gcgcatgaac gaggaaacgc tcgaacgcct ggtgagcggc
    14761 ttcctttttt actcctctcc gcacagcgtc ttcgcgtggc aaggcggcga acccacgctg
    14821 gccggagcgg ctttcttcga gcgcgccgta gagttgcaga aacagcacgg acgcggcggc
    14881 cagtcggtga caaacgctct gcaaaccaac gccacgctgc tcgatgaccg ctggtgcagc
    14941 ctttttcggg aatatcgctt tctgcttggc gtgagcctgg acggcccgga gccgctgcat
    15001 gaccgctatc gcttcaacaa gcagcgcaaa gcgacctgga aaaaggtcat ggagggcgtc
    15061 gcgctgctcc agaaaaacgg ggtcgagttc aacatcctct gcgtcctgag ctcggccaac
    15121 gtggaacgcc ccgccgagtt gtaccgcttt ttccgcggcc tgggcatcca gcatattcaa
    15181 tacataccgc ttgcggagtt cgatggcctg ggcctgccgc ggccgtggac gattacaccg
    15241 gaacaatacg gccgcttttt gtgcgaaacc ttcgacctct ggtggcctga ccggaagaaa
    15301 atccggatcc ggtttttcga caacatcgcg gaagcgctgg cgggcataaa gccgggcaac
    15361 tgcaccctgc atgaaacctg cgacagttat tgcgtggtgg aatataacgg cgacgtgtat
    15421 ccctgtgatt tttttgtgga gcgaaattgg aagctgggca acatcaatgt ggattcgttc
    15481 gccgagatcg cgcgccggca gcgccgctat gcgttcgccg ccaaaaagac ggttccccat
    15541 ccggagtgcc tggtttgcga attcgcccag gtctgccaca gcggctgtcc caagctccgg
    15601 cacggccgaa ataaacgctt cgaggacctg gattattttt gcgcttctta caaagcgatt
    15661 tatgcgaaag ccatccccaa actgaaaccg gagatcgcaa agattctctc gcggggcgtg
    15721 gcttgcggaa ccggtcccgc atgatcggct ctacctggga ttttccgaaa agggaggatg
    15781 cggcgcggtt accaatccat ccgcgcgccg gcggaggcca tggggcgcac ttccaggcat
    15841 cctttgcccg tgggaagcag cttgccaggg ttgaggcggc catccgggtt gaatacggat
    15901 ttcaggcggg cgatcatttc cagggtttct ggcggaaaca gaagcggcat catctcgttc
    15961 ttttccatcc cgactccgtg ttcgccggtg atcgatccgc cgaccgagat gcaatactcc
    16021 aggatctccg cgcccgcctg cttggtccgc tcaaattctt ccggtttgtg gtgattgaac
    16081 agaatgatcg ggtgcatgtt gccgtcgccc gcatggaaga tattgctgat cgtgagatca
    16141 tacttagcgg ccacgcggta gatgaaccgc agcgtgtccg cgatgcgggt gcgcggcacg
    16201 acgccgtcct ggacataata gctcggactc acgcggccga cagcgccaaa ggcgttcttc
    16261 cggcccttcc atagcagatc gcgctcttcg gcgcttcgcg ccacacggac ctcccgcgcg
    16321 cggttcagcc ggcatacctc ctcgatatcg atggtttgag cctcgactgc ttcccgcaag
    16381 ccctccagtt cgatcaacag cacggcgctc gcatccatcg gatagccggc gtgggttgct
    16441 tcttcgacca tgcgcagcat caccccgtcc agcatctcga tggctacggg cgtgatctgg
    16501 cgggcggtga tgtcggccac agtggccgcc gcgtcgtccg gctcgtcata aatcgccagc
    16561 aaagttttca ccagctccgg cttgcgcatg agccgcacga tcactttcgt caccagcacc
    16621 atcgttcctt ccgaaccggt aagcaatccg gtcaggtcat aaccggcgcg gtcaatctcc
    16681 ttgccgccgg tctggacgac ggttccgtcg ggaagcacga tctcgagacc cagcacatga
    16741 ttggttgtga cgccgtaagc gagcgtgtgc ggcccgcccg cattttcgct gacgttgccg
    16801 ccgatggtgc aagcgcgctg gctcgacgga tcaggcgcat agaagtaacc ctgcccctgg
    16861 actgccaggg tgagatccag atttaccacc ccgggttgca cgacggcgcg ctcgttttcc
    16921 agatcgatct ccaggattcg attcatgcgc gcaaagccga tcatgatgcc gccggagcgc
    16981 gcgatagcgc cgccgctcaa gccggtgccc gccccgcgcc ccacgagcgg cagttcgaat
    17041 tctttcgcca gcttcacgat cgccgccacc tgctcggtgg tgcgcggcag cacgacgcag
    17101 tcgggcgtgg atttttctac gctgccgtcg tactcataca acatcaggtc ctgcggctgg
    17161 tgcagcaccg catcgtcgcc ggcgattttg cgcagcttct gaatggcttc ggcgggaagc
    17221 atcgtcgctc cactccgatg atatactgaa aagccacgca tggaaaaggt tgttgtcctc
    17281 gattccggcg gccaatattg tcatctcatc gcccggaaaa tccgcgaact gggggtctat
    17341 gcggaaatcc ggccgctcgg cacgcgagcg cagaggctgt cgcgctaccg cggcgtggtc
    17401 atttccggag gtccggcgag cgtctttgac ccgggcagcc cgcagccgga tcccggtttg
    17461 ttcaagctcc acttgcctgt tctcggcatc tgctacggac agcacctcct ggcccagcat
    17521 ctcggcggca aagtcgaggc ggggaagcgg agcgattcgg ccggcggagg agcccgcgaa
    17581 ttcggcgaag cggaaatcgt cataaaatcc cgcaaatcca tattcgccgg cttgaacggc
    17641 cgcgagcgga tttggatgag tcatggcgat tacgtttcca agctgccgcc cggctttaag
    17701 gttctcggca gcacgcgcga ttgccgcatc gccgctatgg gggacgactc ccgccgcctg
    17761 atcggcatcc agtttcatcc cgaagtcacc cacaccgagc acggcctgca aattctgcgc
    17821 aatttcttgt ttcgggtctg cgggtgccgc ggcgactggc atcccgccga ccagatctcg
    17881 gatctcgaag ccgctattcg ccgccacgcg ggcgggcgca gagtgctctt tttcttgagt
    17941 ggaggtgttg attcgactgt cgctttcacc ctggcggagc gcgccctcgg tcctggccgc
    18001 gtccacggcg tttatgtgga caccggtttc atgcgccacg gcgaaactca ggaaattcgc
    18061 ggcctgttcc agcagctcag actggacggc ctggaggtaa tcgacgcccg ccaatcgttt
    18121 tttcaggcct tgcgcggggt gaccgatccg gaagaaaaac gcaaaatcat cggccggctg
    18181 tttgtggata ttcaggatca agtgctgtcg agaccgtcct accgcagcgg ccagtggatg
    18241 ctcggccaag ggaccatcta tcccgatacg attgaatcgg ggggcagcaa gcatgccgct
    18301 gtcatcaaga cgcaccacaa ccgcgttgat cgcatccagc aattgatcgc cgagaagcga
    18361 atcctcgagc ccctggcgca tttttacaaa gatgaggtcc gccagcttgg ccgcgcgctg
    18421 ggcgtgcccg attccgtgct gcggcggcac cccttccccg gccctggtct ggccattcgc
    18481 tgcgtctgct cgccgcgtcc tgcacgcccc gagccggaac cggcgattgc ggagctcgcc
    18541 gaagaagcag gttacgaggc ctttttgttg ccgctcaaca gcgtgggcgt ccagggcgac
    18601 agccgcacgt accgaaagct gaccgtgctt cacggggctc ctattcacca tgaagcgctg
    18661 gcgcgcctca ccactcggat tacaaacagc tttcctcaaa cgaaccgcgt ggccgccgct
    18721 gtctggcccc aaaatctgat tcccggcgag tggcgcatcc ggcggacttc actcacgcct
    18781 gctcgaatcc gcctgctgcg ccaggccgat cacatcgtga ccaggttttt gcaggaacag
    18841 tttctgtacg atttaatctg gcagtgccct gtggttctgg tccccttctc gcgcaatgac
    18901 ggcgagacca tcgcgctccg gcctatctcc tccgtggatg gaatgaccgc cgaggtggtt
    18961 cacatcccgg ttccgcagtt gaagcagctg gcggccgaac tggtggagtc aggcggaatc
    19021 gatgcggtgc tgctggacat ttccaataag cccccttcca ccatcgagtg ggagtaggcg
    19081 caatccacac gaggagaaaa cgaaaatcat ggcagcaagc caccctacct atgacgatgt
    19141 gaatctcatt ttgcggctgt ttgaactccg ccgggaagaa aaaatgaggc aggcccgcga
    19201 ttggttcggc aagtcgttca aagcgcgtac gctggaagat tttcaggctc tttgcccgcc
    19261 cggatccgac ccgaatgcct attaccgcat ggtagtgagc tactgggaca tggctgcttc
    19321 cttcgtcacc agcggagtgc tcaacgaaga ccttttctat caaagcggcc gcgaactgct
    19381 gttcgtttgg gaaaggattc gcgaactcat cccggagatg cgtaaaactt ttaaaaatcc
    19441 cactcttctc aaaaatatgg agacggtggc caactcgttt atcgagcgca tgaaccggga
    19501 agctccggaa gcgtacccgg ccttttccgc catggttcgc ggaaattagg tcgcggcggc
    19561 taggtttgga agaaccgttc cttcgagcag gaaccgtttg cagccggaga tttcagtatg
    19621 cgccgggagc atgcctgcgc tgctcccggt tggacccggc aaactgactc gcgggcccga
    19681 acaatatctt ttcgagatct gggccgtcgc gttctgctca gggtttcggc cctctggcat
    19741 gagcacgagc cggatgcttc tccgatggaa aatttgattt cctgtaccgg cgtcgtctca
    19801 agctgtggaa aatggaagtt tttggaaatt tgatttgggc gccggggagg attgcttcag
    19861 gggtcttttt tatcgaggtt agtctcgctt tggtgggagt tgcctccccg gcttgttttc
    19921 agtataacac ttcccgctgc cagcgcaagt gcaaaaattt agtttttggt agagccctac
    19981 cgaactacgt atttttctta gccttaacct gcacccccgc ccaatcctct aaaagggtta
    20041 cggcggagca aaagtcctca tcggcatggc cccgggcgat gctggcgcca aacaactcat
    20101 ggaccagggc cgtggagggc aacgggacat tcagttcccg gccggtctcc agcattaaac
    20161 cgatgtcttt gtgcatccag cggagagaga agttcgtcgt aaaatcccgg cggaaaatga
    20221 acggcgcttt ggcgctgatc aaacccgctt tagcggcgct gttatcgagg atctgaaaca
    20281 tcagttccgg atccacgccc gctttcgtgg ccaaaaccat tccttcgata aatccctgca
    20341 tcacgttgct caaaaccagg ttctgcgaga gcttggcgtg caggcccatg cccgctccgc
    20401 cgcaataaaa caagttcttc cccatgatga ggaaatattc ccgggcgcgc tcgaaaactt
    20461 ctttcgagcc gccgatcatg aacgtcagag ttccgccttg cgcgccgctg gtcgagccgg
    20521 tgcagggcgc atccagaaaa tggatgccct tcgccgcaaa ggtctggcca attctgcggc
    20581 tcgcggtagg cgcgatcgtg ctgcaatcgg ccaccaccgt tcccggtttc gctccctgaa
    20641 tgacgccgtc gggtccgacc atgacgttct cggacatggc cgaatctccg acgcaggcaa
    20701 aaatgtaatc cgccgccgct ccgacgtcgc gcggcttggc gcagaaagtc cctttctcct
    20761 cctgcgccag cttgcgggct ttggcttctg tgtggctcca cagcgctact tcatggccgg
    20821 cgcgcaacag gttgcgcgcc atcggatatc ccattagtcc cagacccaag aatcccagct
    20881 tggccattca tcttctcctt ttgtgcggaa tgaaaaacgg aatccccgat tgtacgactc
    20941 cgcggggctg tgcgacaata cccagtttat gaaatcgagg gtgctcgtca gcaagcgcgt
    21001 gtaccctccg gcgattgaat acctccggca gcgcgtagag ctggagtaca acgacagcaa
    21061 cgagagctac tctccgcaca aactgattga gaagctcaaa ggcaagcagg gcgttgtctg
    21121 ccaggtcacc gataaattca cgccgggcgt gatggatcag ttgccggatc tcaaaatcat
    21181 ctccaatatc gccgtgggag tcgataatat tgacttgcgt gcggcgaccg cgcggaagat
    21241 tctcgtcacc aacacgcccg acgtgctcac cgatacgacc gccgatttcg ccttcgcgct
    21301 gctgctggcg gcggcgcggc gcgttgtgga aggcgacgcc ttcatacgct ccggccaata
    21361 taagcaatgg ctgatggacc tgctggtcgg ccacgacgtc caccatcgga ctcttggcct
    21421 cgtgggaatg ggccggatcg gccaggccat ggcgcggcgc gcccgcggct tctccatgcg
    21481 gatcttgtac tacgattctt cgcgccgccc cgccgaactg gagcgggaac tcggactgga
    21541 atacacgccg ttcgatcagc ttctgtccga aagcgatttc gtgagcctgc acgtccccct
    21601 ggcgccggag acgcggcaca tgatcggggg cccgcaattt gcgatcatga aaaggacagc
    21661 cattttgatt aacaccgctc ggggccctgt ggtggatgaa gccgctctgg ccgcagcgct
    21721 cgcgggcggg cgcatcgcgg gggcgggtct cgatgtttac gaggaagaac ccaagctgca
    21781 tccaggcctg caacggctca agaatgtcgt tctcgcgccg cacatcgcca gcggctcgca
    21841 tgacacgcgc atccgcatgt gtatgctggc tgcggaaaac cttgtggccg gcctcagcgg
    21901 caaacttccg cccaatttgg tgaaccgaga gttgatgccc gcatgaagga ccgccttcgc
    21961 cggctctttc tggaaaccct ggacaggctg tccctggagc ggatcattcc gcatcgcgtc
    22021 agcgtccggg atggcgtcat ggagatcggc ggcgacagaa tagagctgcg cgcctataaa
    22081 aagatcatca tcgtggcgat cggcaaggcc gcctttcaga tggcgcggtc tgtgaaggcg
    22141 ctgcttgagc cggccgtggc ttccggcgtc gtggcgagct ccgagccggc cgaagttccc
    22201 ctgcctttcc tgttgcacta ccgcggcggc catccctatc cgaacgccga aagtctgcgc
    22261 gctgctgaag tgattctgga actgttgcgc cagctcaatc aggaccacct cgtgatctat
    22321 ctgctgtcgg gcgggggatc ggccgtcgtg gaaaagccgg tcgacccttc tatttctctc
    22381 gaagatatgc gcgccttcta ccgcgttctg gtaacctgcg gcgccaatat cgtcgagatg
    22441 aatacgctgc ggaaacactt ttccgccacc aagggcggca gaatggcgta cgcggcccac
    22501 cctgcgcgtc aggttaccct gtacgtttcg gatgtgcctg tggaccgccc ttccgcggtg
    22561 gcttcgggcc ccaccatgcc cgatgaatcg accgtcgctg attgctggga gatcgtcaag
    22621 cggctcgatc tgctcccgcg gtttccagct tctatccgca ggatgttcga agcgggaacc
    22681 attccggaaa ctcccaagcc aggcagctct cgtttcaaac gctcgacctg gcatgccctg
    22741 ctcacgaccg aacaggggac cgagcagctc gccgccgcgg cgcgcgagca aggctggatc
    22801 gtggatcaag atctctcggt cgatgattgg cccttgatcc cggccgccga gcacctgctc
    22861 gagagactcc tccgattgcg tgccgagaat ccaaggcgca ccgtggccat tgtgtccggg
    22921 ggcgagctga gttcgcccgt gactggggat ggcgtcgggg gccgcaacca ggcctttgtc
    22981 ctggcctgcg cccagcgtat tgcaaagaaa aatatcgctg tgttgagcgc cggtacggac
    23041 ggcattgacg ggaattcgcc tgcgtccggg gccgtcgccg atggtgagac gctggaccgc
    23101 gcggcgcagc tcggactctc gccggaagat tatttccagc gctcggattc ttttggattt
    23161 ttttcgcggc tggatgacct ggtggtgaca ggcccgaccg gaaataacat tcgcgatctg
    23221 cgcttgctgg tcgcctggta gctattcgat ggcctggccc cggcccagca tccctaaaag
    23281 cagcaagtcg gaaggccgga agcgcatcgc gccgacattc ccgagcgctt cttctttttc
    23341 caaaacgtgc ttgagaagca gggaccggaa ttggtccgga gaaacgccca acgcccgggc
    23401 tgcctccgtt tccgtatatt ctgtcttgcc tggctgcaat cgaatgttca tggcggcccg
    23461 tccttaggcc taaccttatt gtttctcggg caattgtaag tggccgccgc accggctcga
    23521 aattagggaa agttcctaca cccgcggcat tgttgcccga ttcaccttag gccactggtt
    23581 ccattgcctg gtactttctt ctttattcgt taaccggggg gcggtttgtg gccggcggag
    23641 ggcgactgct tgtgcgccag gcggccctcc accagcatgc tcaacagcag caagtccgac
    23701 ggacgaaacc gcatgatcgc gacattcacc aggctttcct cttcctcgac aacatgtcgc
    23761 agcaacaacg atcgaaactg ggcctgcgaa atacccaagg cgcgggctgc ctccgtctcc
    23821 gtatattcgc tcttgcctgg ttccaatcgg atgttcatag gttgtccggg ttccggtccg
    23881 attgtagcca gagccgggct tcgggccata ttgggcaaag ctcttatatg cgcccggcgt
    23941 tgttgcggga tacttaccgg atgttggtac ttttcctgta agactaatga tctagaccgg
    24001 gagtcgtaac cgaggcaagc gcgcgctcgc tttccgtttc ccttctcgcc cccggccgcg
    24061 acgccgcttc ctgctgaaag cgatgcggct tcctgctaag cggagaaaag cagccgccag
    24121 gcggaaatgg ccagcgcatg agtgaggctg gaggccacaa cgctgcgggt ttcccggtac
    24181 gcctgcccgc aaaaccatcc aagcagcgcc ccgagcgcgg caaaacgcca gttgggaaac
    24241 tcccgaaagg gcagatggca gaggccgaag attatgctcg tggcagcttg cgccgccgtg
    24301 gcgccgccta gattcgctgc gctcaggttt tgtaataccc ctcgaaagaa aagttcttcg
    24361 gagagggcga ccacggcgta catcccgaga aaagttccag ccgcctgcaa cggatagagc
    24421 catggctccg tacgcaaggg ttgaaaatgc gccgcacctg tcagtaaggc gagcggcgcg
    24481 cccggaatca agaaaagaag taattgcttg attccggttt tccattcctc ccgggacgtc
    24541 tggaaccgaa agcctgttcc ttccacctgc cgcaggcaca aaaaagccat cgcgcccagc
    24601 ccgatgatca tcacgtggcc catcgattct aaccgcagcc ccggtatcgg actcagatat
    24661 acctgccgca gtacccggct cagcagcgcc gccgctatcg ttccggccgc cagcacatcg
    24721 ggccaggtca gccgggagcc tttgaccgga aaaacaacga aaatgaaacc cagagtcccc
    24781 gcgaggccgc ccagcttcgc cagcgcagaa aattgaaaga cgccggccgg aagactgtaa
    24841 atcagatatg gtgttatcgc ccacacaacc gcaatggcgg ccagccgggg aggggaaaaa
    24901 cggcgcgcca gccagcctct catctcgggg aagccgagtg cgagatagaa tgaaatttgc
    24961 attaagaatg cggcaacaat gggaacaacc attgccccgg gtaagtggcg agatgccgca
    25021 tagaccgccg cgcccagcgc cagtaaggca tccacgatca gccaggttcg gagaaatgcg
    25081 cggggcatcg caccatctta accaagtttg ccgcgccgcc ctctgggcgg cagcttgcgc
    25141 tctggcggtc tcaagcgccg tctcgccgct ccacgggcag gagccggagg aaagcctgat
    25201 ccgggagatc gccgcgaaag caagtcaggc ggaagccgcg cgagcccact atacctaccg
    25261 ccagacgatg atctttcagg agctcgatgc gcacggggcc gaggtgggca ggtaccagga
    25321 agtccgagac gtcatttttt ctcccggcca gaagcgcagt gaagtcctgg tgggccgccc
    25381 gcacagggac ctgaaacgat tgaaattgac cgacgaagac ttccgcgacg tgcgcgaggt
    25441 caacccgttc gtgctgaccc gcgacgaatt gttctactac caatcgaaat tccaggggcg
    25501 ggaagcgatg gacggcgttg cctgtatggt ctatcgcatc tcgccccggc agatcctgga
    25561 cgggcagcgg ctcttcgacg gtttgatctg ggtcagcgag aaagaccggc aaattgtccg
    25621 cgccgaaggc cgccctgtgc cgcaaattta ccgcagcacc ggcgaaaacc tgttcccgcg
    25681 cttcacgacg atttaccgcc cggttgacgg ccagcactgg ttccctgtgc gcaccctggc
    25741 ggatgacacg ctgtatttcc gcagcggccc gcagcgcgtg cggatcacca tccgctacga
    25801 cgaatacaag cggttcagct ccgacacgac gatcgaatac gaaaagtagg gcggcgcggc
    25861 gcctgatcat aactggctcg cgcggcggaa gcggtaggtc accgagacag tcgcgtaaga
    25921 cttgaggcga aagccggaga tgcgctcgaa gggctctgct tcgcccggca gggccagcgt
    25981 ctcgagggtt tgcccggcaa acccgcgcac gaggaaaagg ttgattccaa cgtcgatccc
    26041 cttgctatcg aacgggcgaa tcatcgcgcc tcgaaactcc tgtacgggca cggcagcgaa
    26101 gccgtcgccc aggggcttgc gtcccacgta ctcgtactcg ccccgtgcgc gcagcttgaa
    26161 cggcaaacgt tccaccgttc ccagcacatc gccgatcaaa cgcggggcct cgggggtggg
    26221 ttcgccggtc aggcgatccc tggcgtccgc tttggcgaac agcacctgga gcgaacccag
    26281 cccgaagtcc cgcctcgccg agacggtcat ggagcggatc aaggccggcc cgacgtcctc
    26341 ttgcaggccg gtgtcgttat cgatccgcgc cagttgctgc gcggttgtta catgggcaag
    26401 cgtcaccttg aattccgtgt gcgcggcggt ttttctaatc accaattggt aggcgcgagc
    26461 cttgctaaca acagttcctc cagcgatcgc tgtggttcca atgcgcgggt cgtctacgtg
    26521 aaacgactgg ccgtaactca aggcgatccg gggcagccag gcggctccct ccggtgaaaa
    26581 agaaatggtc cccttgggtg aggtcacccc ttgttcccgc tgaaaggact gcaccggcgc
    26641 cagccggtcg cggttgtcga aagcaacctc atcgcggcgt gagtgcgccg tcaagcgccg
    26701 cataaggcgt gtagaattgg atcgtgatgt cattcgaggt caggggatag aacgccgcgg
    26761 ctgcttcatt gaacttgtcc agatcgagat tgcggggcgc ctcccggcga tagtcggctc
    26821 cgagcaaaag cgaaaagaat cgggcaatcc ggtaggcgta attcacgctt tgggacgttg
    26881 ctgtccggtt ctcgctctgc cggatcaggc cgccgccgaa gtccggccga acatcgagat
    26941 aatagttccg aaaatagccg gagaactgaa gctggcgcgt ggcggtgaga cgccagacgt
    27001 cgttgacaat cgcaatgccg gagcttgcct cttcgcgctg ccgcggatcg atggtgtccc
    27061 cgggtacggg cacgccgatc ggcgccatgc ccggcagata cgaaaagccg tgatagccga
    27121 ttccgtagac ggtgaggtcg tggcggccga attgaaacac ccgtgatccg ttgagtttgt
    27181 actgcttgcg gcgctcgggc cggcccagaa atccgtttcc caaggaaagc tcgagccccg
    27241 cccaagcctt ggtttcggcg ctcgccgggc tccagccggc gacgaggttc acatcgtggg
    27301 cgtcagccgt gaatcgcacc attggatgga ggcggtcgcg cagcccgaaa atcattgacg
    27361 cgttcattga gttgttgccc tcccggacgt tgaatgaacc tccgtcggtc tgcacactct
    27421 cgatcgcgat aggaatgagt acatttggat ccgcgtagcc attgccatga gcgttggcgg
    27481 gcaggttatt gggaaacagg tagccgccga cctggaagaa ctggccgatc ggctcgccgt
    27541 gatctcctgc gactcccggc acgaaatact gcggaggttt gatcccgccg gcaggcgact
    27601 ctacgggcat gcccgggatc gataggggca tgccgggccg ccccgggttg gcgtccagga
    27661 tttcctggcg gatcagcacc cgcgaagccg ggtcgggaga aaaaattccg gggccttcca
    27721 cctcctctgt gacggtcacc gactggcttt gcacggcgag ctgtgaaaaa tgcaagtcgg
    27781 cttccagcag ggcgtttcca gaaacgcgga tgtcgcggga aacggacgga aaacccgccg
    27841 cctccgccgt aagccggtac tcgcccgcct ccaccgaggg aaacaagaat cgtccctggc
    27901 cgtccgtctt gttctccatc tggaatacgt cggagcgccg gactagcgtc acctcggctc
    27961 cagcaaccgc tttgccctgc ggatcggtca cctgtccggc gactccggag gaggcgcaag
    28021 cccaaccgga aaacaggacc aacagggcgg cctgttttgc cgccaggaaa agagacggtg
    28081 gaagcgctcg gcgtacgtcc ggcgccaggg ggaatctctc cggcgtcatg attttgcaag
    28141 taacacgatt tttaaattcg tgctacgata tcacgtccga agaagcattg caagagcgaa
    28201 ttggcacgcg cctcccgtac ggcgcttgtc ggcatgcggc cgccgccgca acggcctgcc
    28261 cgggagaggc gcgcggggat gcgtcgccgc cacggaccaa gacggccgga agcgccggac
    28321 gcttgcgcat cgaagacggc gctcggctac catagggaga aatgatcaac aaacgcactt
    28381 tggtcgcgat gggcgccggc ttggtcgtga cggcggtcct ggtggccgca atcgtcttta
    28441 tccagcgggg ttcgacgccc cggctggtcg gtgaaatcag ccgggtccgg acgctcgaaa
    28501 tggacccgtc aagctcggtc gcgatcgccg atttccgatt caccaacgac agccattacg
    28561 cattcatcat cgcgaccatc gatttatcgg taattgattt caagggcgag acccgcctgg
    28621 ggcaggttat ttcggcttcc gacgccaacc ggctgttcca actctttccg gcgctgggac
    28681 cgaagaccgc tgaaccgctc attatcaaga ccaaggtcgc tcccggagct tctgtcacgg
    28741 ggatggtggc ggcccgcttc gagctctcca agacagatct cgacgcccgc cggaagatca
    28801 tcctgaccat cacggacgta gacggcccgg tc
//
LOCUS       DASREK010000158         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_411294, whole genome shotgun sequence.
ACCESSION   DASREK010000158 DASREK010000000
VERSION     DASREK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411294"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..685)
                     /gene="purN"
                     /locus_tag="VFA54_08030"
     CDS             complement(86..685)
                     /gene="purN"
                     /locus_tag="VFA54_08030"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZS50792.1"
                     /translation="MKRLGILLSGRGSNFEAIADNAAAGVIPAQIAIVISNNPNAAGL
                     EKARARGIPAAAIPSKGMEREPYDRLVAAELQKRQVDLVCLAGFMRLLSAWFVRQYPS
                     RILNIHPALLPAFPGLEAQKQALEHGVKISGCTVHFVDEFLDAGPIIAQAAVEVRDDD
                     TEETLSARILAEEHRIYTEAINLVLSGRYRIEGRRVLRT"
     gene            complement(682..1722)
                     /gene="purM"
                     /locus_tag="VFA54_08035"
     CDS             complement(682..1722)
                     /gene="purM"
                     /locus_tag="VFA54_08035"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZS50793.1"
                     /translation="MRASQSVSYASAGVDIDQANRVVQRIKHLARGTFNKHVLTDIGS
                     FGAVYSLAGLRSKRPVLISSADGVGTKLKIAFATGIHNSVGRDLVNHCVNDIAVQGAT
                     PLFFMDYFAAGRLDSGVADQVVSGLAEACRENGCALIGGETAEMPGFYPAGEYDLAGF
                     IVGEADRGKLLDGRSIRPGDALLGLPSTGLHTNGYSLARKLLFDMAGYGPGTFLPELG
                     VKVGEELLKVHRSYLAPIRLLVKQGLLRGAAHITGGGITENTPRMLPPGLAARLRRGS
                     WPVLPIFDFLQRIARVEEEEMLRTFNMGIGMIVAVPKGSEARVMRALASIREPAYIMG
                     EVIRGKRGVVWV"
     gene            1986..>2279
                     /locus_tag="VFA54_08040"
     CDS             1986..>2279
                     /locus_tag="VFA54_08040"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS50794.1"
                     /translation="MKSISVRFFVGSLLAAACAIAQVNTASLTGLVRDSSGGVIPNAT
                     VTAKSKTTGAEQRVTTDQTGYYAFSSLPAGDYTLTAEQTGFNREVRDLSLDPSQ"
BASE COUNT          450 a          722 c          675 g          432 t
ORIGIN      
        1 agccgctccc gcagctcctc ttcccggatg atttcggcca tccccttgcg caggtaagct
       61 agctgttcgt cgatgccagg ctgggtcacg tgcgcagcac ccgccttcct tcgatgcgat
      121 accggccgct gagcaccaga ttaatggcct cggtatagat ccggtgctcc tcggcgagga
      181 tgcgggccga cagagtctct tctgtatcgt cgtcgcgaac ctccaccgcc gcttgggcga
      241 tgatggggcc ggcgtccagg aactcgtcca caaaatggac cgtgcagccg ctgatcttca
      301 cgccgtgttc gagcgcctgc ttctgggctt ccagcccggg aaatgcggga agcaaggccg
      361 gatggatatt caatatccgg ctggggtact gccgtacaaa ccaggcgctc agcaagcgca
      421 tgaagccggc caggcacacc aaatccacct ggcgtttttg cagctcggcg gccaccagcc
      481 ggtcgtaagg ctccctctcc atgcccttgg atggaatcgc cgccgccgga atgcctcgcg
      541 cccgcgcctt ttcaagtccc gccgcgttag ggttattact gataactatg gcgatctgcg
      601 ccgggatcac gccggcggcg gcgttgtccg cgatggcctc aaaattggat ccccggccgg
      661 agagcaggat gcccagccgc ttcacaccca caccaccccg cgcttgccgc gtatcacctc
      721 gcccattatg taagcgggct cgcgaatgga cgccagggcg cgcatgactc gcgcctccga
      781 ccccttggga acggcgacga tcatccctat gcccatgttg aatgtgcgca gcatttcttc
      841 ttcttccacg cgggcaatgc gttgcaggaa atcgaaaatc ggcaacaccg gccacgatcc
      901 ccggcgcagg cgcgcggcca ggcccggggg cagcatccgg ggcgtatttt ccgtgatgcc
      961 gcctccggtg atgtgggcgg cgccgcgcag caggccctgc ttgacgagca ggcgaatggg
     1021 agcgagataa cttcgatgaa ctttcagcag ttcctcgccc actttgacgc cgagctcggg
     1081 caagaaggtc cccgggccat agccggccat gtcgaagagc agcttgcggg cgagcgaata
     1141 gccgttggta tgcagtcccg tagagggcaa gccgagaagc gcgtcgccgg gtcggatcga
     1201 ccgcccatcc agcagcttgc cgcgatcagc ctcgcccacg atgaagcccg ccagatcgta
     1261 ttcgcccgcc ggataaaagc ccggcatttc ggccgtttca ccaccgatca gagcgcagcc
     1321 attctcacgg caggcctcgg ccagcccgct tacaacctgg tccgcaacgc cgctgtccag
     1381 gcgtccagcc gcgaagtagt ccatgaagaa cagcggtgtg gccccttgca cggcgatgtc
     1441 attcacgcaa tgattcacca ggtcccgccc tacgctattg tgaattcccg tggcgaaggc
     1501 gatcttcagt ttggtgccta cgccgtcggc ggaactgatc agcaccggac gttttgagcg
     1561 taaaccggcc agcgaataaa ccgctccgaa gctgccgata tcagtgagta cgtgtttgtt
     1621 gaaggtgccg cgagccagat gcttgatgcg ctggacaaca cgattggcct gatcgatatc
     1681 cactcccgcg gaggcgtaag aaacggattg gcttgcccgc atggattttc cattataggc
     1741 gatcccgcgc cgcagcgatt cctgcgccgt gctcgcgcca atcgctgccg ggaagcactt
     1801 caccccctgc ccgggccggc gtatcgcgta aacgaaaaag aaagatttct gtccatgaaa
     1861 tttaacggaa ccgcaatggt gaaaggcagg cttttgtgat accatcgctc tccatagggt
     1921 caagttttca cagggtcttg ggcagttagg ccggaagcgg ggaagtacat acaaggggag
     1981 aattcatgaa atcgatctct gttcgatttt ttgtgggcag tttactggcg gccgcttgcg
     2041 cgatcgccca ggtgaatacg gccagtctga cgggtttggt gcgcgactcg agcggcgggg
     2101 tgataccgaa tgcgacggtc accgccaaaa gcaaaaccac cggcgcggaa caaagggtca
     2161 ctaccgatca aacgggctat tacgcatttt cgagcctccc ggccggcgac tatacgctga
     2221 cggccgagca aaccggcttt aaccgcgaag tccgcgatct cagcctggac ccgtcgcaa
//
LOCUS       DASREK010000159        17400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1402669, whole genome shotgun sequence.
ACCESSION   DASREK010000159 DASREK010000000
VERSION     DASREK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17400
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402669"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFA54_08045"
     CDS             <1..977
                     /locus_tag="VFA54_08045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZS50795.1"
                     /translation="RRNRQAFDWVELAPRGVVDVSSVNAATEILGTKMAFPIMCSPTS
                     GHALLHPDGEAGTHKGATAASNTPMIVSNAASLPIDKIAEAAAGPLWFQLYPREDLDA
                     SREILDRAQAAGCQAVAMTVDQQAAFYERPLHDRHLGGNLSPSVSVAAGRTGRGRGRI
                     PPPRNPYRVPEYRLWYSWKYVDQIRPFLKVPLLAKGILTAEDARLCIEHGMNGIIVSN
                     HGGRSMDYGPSTLETLPEIVDAVQGRIPVLIDGGFRRGSDILKALALGAKAVCLGRTI
                     RWGLGAYGAPGVQRVLEILQTELVLAMAATGRPTLDSIDSQLVRTNFV"
     gene            974..2065
                     /locus_tag="VFA54_08050"
     CDS             974..2065
                     /locus_tag="VFA54_08050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZS50796.1"
                     /translation="MSKVRFSRRQMIAGFGSLAAASPLLRGQQPLAGEPPGRITPRDE
                     LVNVFECEAVAQRKLASTEYSSISGSDRRAFDRITFRPRMMVNAMGLDLTTELFGEKM
                     FAPILVGPASHQQRYHPEGELATVRGAAAAKALMVVSSRSSVPLDKIAAEAKTTLWYQ
                     VFPEPDVAAVRSRIQNAVKAGCKAIVITVGTPYQPAGASGAPNPAKLAIMADPAINWS
                     VIDQLRQASSAPVVLKGIMSPEEAQIAVQRGVQGIVVSNHGGRFTPGLASPIEMLPAI
                     ADAVGGKVPLLIDGSFRRGTDMLKALALGARAILLGRPPLWGLAAYGAEGVQTVMEML
                     QTELARNMAMCGKPTIKEIDRSAVKIHRA"
     gene            complement(2176..2478)
                     /locus_tag="VFA54_08055"
     CDS             complement(2176..2478)
                     /locus_tag="VFA54_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50797.1"
                     /translation="MAAELLEVRCPCCKAVLQVDAETQLVIAHQPPEKTPPIEDLKEA
                     VEKLKGEERKRDQIFRQQFEAEKTHGKVLEKKFDQLLKQAKAEPAGKPGIRDIDLD"
     gene            complement(2546..2836)
                     /locus_tag="VFA54_08060"
     CDS             complement(2546..2836)
                     /locus_tag="VFA54_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50798.1"
                     /translation="MSERGVYRLAGAMLLQAIQDASSSSSGRRSSALRWIRSSDDSCF
                     SFSFVCRILNRNPEEVRRYCEQKAAERGKITVPYLEGWQDRTSWPSFHLTFR"
     gene            complement(2950..3531)
                     /locus_tag="VFA54_08065"
     CDS             complement(2950..3531)
                     /locus_tag="VFA54_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50799.1"
                     /translation="MTEIQKVSVKILSDAPQTLDLTPFLSIFGRWRADAAHPAQWVDL
                     ADYAHMARGAGVVLIGHICNLSFDMGTAAPGVLYFRKKGLEGSNQQRLWAVFRDALAI
                     SRCLLREPEFPPGVHLETGRLELAFNDRLETPNTEDTDRELRPAISAVLDGLFGRDSY
                     ELERQRDPAKLYGFSVRAANPPAWGELIARLGL"
     gene            complement(3528..5669)
                     /locus_tag="VFA54_08070"
     CDS             complement(3528..5669)
                     /locus_tag="VFA54_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50800.1"
                     /translation="MATSILTERDRWAHLPPAVKEELDVYETELHRMQQGLISEKVFL
                     ELRLRFGVYGQRQPGVQMQRIKIPLGMLTARQMDRIADLAEEYADGVCHITTRQDVQL
                     HFVNINDTPNLMRRLAEAGITTREACGNAVRNVCACPKSGVCQEEVFDATPYARAMAY
                     FMLRHPDAQNFGRKFKIAFSGCEDQACALALMHDIGAIAQVREKQGRLERGFKVYVGG
                     GLGPLPYQAKLFSEFVPAAEMLPLAQAIGRVFARLGEKKNRGRARMKFLVGQLGIEEF
                     VRLVLEERQKLPPDPRWTDYLEAAENFAEKPLRPPSDLDLSGRSPEFIRWLRTNVRPQ
                     RQPGYTTVTVFLPLGDISADQMHGLAALCRRFVNDTIRTTVEQNIMVRWVASGDLPDF
                     YDGLRELDLAQIGAGTLGDVTACPGTDSCKLGIASSRGLAALLHEKFHNGLSDVAART
                     DLNVKISGCFNSCGQHHIADIGFFGSVQRKGGHVAPVFQVLLGGEMEGNASAYGLAVA
                     KVPSQNAPAVIKKLSDLYTRERVESERFSDFVRRLGKARIKEELEELTVLPSYEEAPA
                     YYKDNRQPWDYHMSTEQGECAGEVVDQSEFMLEDADRLVFEATLDLDAGRYDEAAAKT
                     WAANLKAADALLFQKGLLLSDNYNTVEKFRELFFDTGEFYGTFAENFFRAAQEGNQGL
                     GPEKTRRRVEEATLFVEMAQTVYSRMAGAKV"
     gene            6139..7281
                     /locus_tag="VFA54_08075"
     CDS             6139..7281
                     /locus_tag="VFA54_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZS50801.1"
                     /translation="MEFGYTSEQQQLRRMVREFAESEIAPHVREWDENQTFPAEVIRQ
                     LGALGLMGTIFPEELGGAGMGYIEYAIVIEELSRVDGSVGIIVAAHTSLCTNHIYKMG
                     TPEQQRAYVPKLASGEWLGCWSLTEPEAGSDAAGTRTTAARENDCWVLNGAKTFTTNA
                     HHADVCVAMAVTERAQGHHGISAFIIPKGTPGFRLGRKENKLGLRASDTGEVIFSDCR
                     LPASQLLGKAGEGFIDSLKILDGGRVSIAALGVGMAQGAYEAALRYSKQRRQFGRPIS
                     EFQTVQNKLADMATEIDAARLLTYRAGWMLDQGQRNTKEASMAKLFSSEVAVRVANEA
                     VQIHGGYGFIKDYPVEKFYRDVKLCTIGEGTSEIQRLVIARQLLKE"
     gene            7587..8573
                     /gene="meaB"
                     /locus_tag="VFA54_08080"
     CDS             7587..8573
                     /gene="meaB"
                     /locus_tag="VFA54_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HZS50802.1"
                     /translation="MMNGKREESRLECNQPDSREVGAWIQKIRSGDVRALARALSEVE
                     NGGPRAQALLAGLFPFSGRAWRVGVTGAPGAGKSSLVDCLARELRREQKTVGIIGIDP
                     TSPYTGGAILGDRIRMQAHASDPGIFIRSMATRGFLGGLTRATGDAVLVLDAAGRDVI
                     LIETVGVGQDEVDVVKVSDAVALVLAPGMGDDVQALKAGVLEIADVLVINKSDREGSD
                     RLEQELRAMLEMARRPDGWVPPIVRTVATESSGVSELLAALRTCHFPGRRVSNWRAQL
                     LEMLQCRLMDRLIERALGNGELDAAAAAVASRQRDPYRFVEEVIERYERCAD"
     gene            8554..8982
                     /gene="mce"
                     /locus_tag="VFA54_08085"
     CDS             8554..8982
                     /gene="mce"
                     /locus_tag="VFA54_08085"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HZS50803.1"
                     /translation="MSDVRIDHLGIAVRSLNAALSFYERALGLTAYGQEVVEQEKVRV
                     AMLPAAGGGPPERLRPRIELLEATAEDSPVARFIAKRGEGLHHLALRVPNLDAAVDRL
                     RTAGARLVSQSVQAGAGGHRYIFVHPSSTGGVLLELIEGD"
     gene            8982..9842
                     /gene="mtnP"
                     /locus_tag="VFA54_08090"
     CDS             8982..9842
                     /gene="mtnP"
                     /locus_tag="VFA54_08090"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZS50804.1"
                     /translation="MTARIGVIGGSGFYSMEGFAAAKEEALETPWGKPSDNYILGELS
                     GRPVAFLSRHGRGHRLSPSEINYRANIYGMKMLGVERILSFSAVGSLKENLGPRDFVI
                     PEQFFDRTKDRPSTFFGEGLVAHISFADPICPQLAGVAEEACRAAGVRVQRGGAYVCM
                     EGPAFSTKAESNLYRSWGMDVIGMTNLQEAKLAREAEICYVTIAMVTDYDCWHPGHAA
                     VTVEDILGHLAANAHNAHRVIAEAVQRMPAERTCRCGSALAHALLTDRNAIPPATRRK
                     LALLIDKYLN"
     gene            complement(9834..11933)
                     /locus_tag="VFA54_08095"
     CDS             complement(9834..11933)
                     /locus_tag="VFA54_08095"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50805.1"
                     /translation="MAQAASSLARAIEHHRAGRLRDAERIYHRILARNPNDSDALHLL
                     GLVTFQTGDFQGAIGLIREAIRQKPSEPVYYDNLGKIFQAQGRAAQAAECFEAVIRCR
                     PADAAALVNLGNSYYRLGRPDDAIACYRRALEQQPDCLDAWINLGHTLNRTGRSEEGI
                     GWLRKALDRKPDCVEALLNLGAVLGDRGESEAAAALARRALSIKPDFAEAHHNLGVAL
                     RSQGRLSEAAASYREALRLKGDRAETASGLGVVLLEQGRLDEAAAAFREALRIDPRFA
                     VAHSNLLFCLNYFSDLDPSQIYTEHLRWEKEQAAALLPLRKPHANHPDPGRRLRIGYV
                     SPDFRSHPVAHLFGPLLAAHDRRQFECFCYSSTLQPDAVTRSLQMLAGAWRDVHALTD
                     EQLARLVREDGIDILVDLAGHTAGSRLRAFAAGPAPVQVTYIGYPNTTGLSAINYRIT
                     DAWADPVGAEGFHSEELVRLPSGFFCYQPLSDAPPVADQPFYRSGVFTFGSFNNRSKI
                     TADVVAVWAAVLRQSSNCRLLLKAKALGDEGTRQELLRMFAAHGVEPGRIELAGVIAN
                     AASHLAEYGRVDLALDTFPYNGTVTTCEALWMGVPVVALAGRTHAARVGVSILSRIGL
                     SEFVTGSIDEYIAGAVHWSANPGRLRELRAGMRERLVRSPLCGAALVARELEQAYRQM
                     WRRWCRQAGASGEGLSI"
     gene            12269..14092
                     /locus_tag="VFA54_08100"
     CDS             12269..14092
                     /locus_tag="VFA54_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZS50806.1"
                     /translation="MRAVHIAVLLGAAVRLLTAQTVSFISRGAFAAGASPSALAVADF
                     NGDGLLDVAVANGGFSSTVSILLSLGQGQFRSPMTFAAGASPRSILTGDFNGDGKPDL
                     VTANSGSNDVSVLLGSGAGIFLPPLRFLAGEGPRFVAAGDFNRDGKPDLVVANANSNN
                     VSLLLGLGRGLFLPPINFAVGRSPSSLVVGDFNRDGKLDLAVANSGSNDVSILLGSGD
                     GRFLTALNFPAGSGPVSIAAGDFNGDGIPDLAVADGGFADNVSVLQGIGNGVFQAARN
                     FRAGGGLAFITTGDVNQDGKPDLIVANGGFGNTVSILLGAGFENFLPPISIAAGSSPA
                     GIALGDVNGDRMPDLLVANSNAGQVSVLLNSSLGVAPSLHAISAASGAPVVAPESIAS
                     VFGPSLAGITQSAAAEPLPKTLGGITVEVQDSRGTRRPAPLFFVSPNQINFEIPADLA
                     PGEAAISILTDTGVSFSATAQVNLVAPGLFTADGTGNGVAAAVVSRSHLADPTEEFLP
                     AFQCVDGQCEGVPIELSPDTDVFLSLFGTGLRNRSSLSQVTVAINGVPAPVLYAGPQG
                     SFTGLDQVNVLLPQSLRGSGNADVILTVDGQTANPVRINIK"
     gene            complement(14476..16878)
                     /locus_tag="VFA54_08105"
     CDS             complement(14476..16878)
                     /locus_tag="VFA54_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZS50807.1"
                     /translation="MAFSIVTNVASLLAQDNLRITSNFQQTTIQRLTSGLRINSSADD
                     AAGLAIANSFRSDIAVLQQGVRNAGDGLSTLQTIDGGINNISLLLDRARTLAAQSASG
                     TFTGDRGPLSSEFQSVLNEIDRQAQAIGLDQGGTFAKALSVFIGGGRGHAGDPSSVAI
                     NNGTVGIDLSNSTVDARSLGLKGLQALGGVAGTTDIGPGSAATNVAAILANSTNTASE
                     AVSGNTVFYFAGPGFSDVNKIAVSVNLNGVVDTTTLATAINSAIQAAGNGTSQAAAAF
                     KNANIKAVINTDSTGKQQLSFTSATTAFQVEAGDRLANALLGNYTSSSDPTGLSTAET
                     LTGAGNLANATAAETVTLKVAGPGFTNPQFVTLNIGASDTAATIVSNLNAALAANSTL
                     AAAGYSASGSAGNPLVISNTRGEAFQVYVAGDTNNYLGLGTALTGASNAVTYTTITGG
                     ATDATVAATGSISMLIEGAGGSGITISATGGAGLTTQNLADQLNSAITANPTLQAAGL
                     KVTVTAGTSAATGSLNVISSNGTHFQLTSTTTNAVTGFGTIAGALTTGTLAEPTQLTF
                     DSGGAQGTSGTSATAPTAFTPLFYGNDVQNLVFTTTANGTQHSLSIQLANANAATLDQ
                     ALNTINAKLQQSNDPALQSIVAVKEKISGNAEGIKFLSSSSTPFTVGIGNTASGNGVS
                     TAGNNSLLTSSALPGGSTTDISSQANAEAAVSSLATAVTLLAGAQATVGKGQNQLNYA
                     VNLASTQVNNLSVAEGRIRDADLAAEAANLTKASIALQSGIAALAQANSAPQAVLALL
                     RG"
     gene            complement(17008..>17400)
                     /locus_tag="VFA54_08110"
     CDS             complement(17008..>17400)
                     /locus_tag="VFA54_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50808.1"
                     /translation="RSRLNALAAQRVRLLEEAQEKRRQSRAARLALLSRSTFPAADLK
                     YAHDYAGSLERSREETLGRAEQVEQRRREQREIVIEARRKKRLLEILRARKLARYSKA
                     AGREQENLIADLHLAKLNRESADFAPPS"
BASE COUNT         3257 a         5255 c         5548 g         3340 t
ORIGIN      
        1 tacgccggaa tcggcaagca ttcgactggg tggagctggc gccgcgcggt gtggtggacg
       61 tcagctcggt aaacgcggct accgagattc tcggcacaaa gatggctttc ccgatcatgt
      121 gctctcccac ttccggccac gccttgcttc atccggatgg cgaagcagga actcataagg
      181 gcgccacggc cgcgtcgaac acgcccatga tcgtgagcaa cgcggccagt cttccgatcg
      241 acaaaatcgc ggaagccgcc gccgggccgc tgtggtttca gctttatccc cgggaggatc
      301 tggatgcgag ccgcgaaatt ctggatagag cccaggcggc aggctgccag gccgtggcaa
      361 tgactgtaga ccagcaagcg gccttttacg agcggccctt gcatgaccgc cacttgggag
      421 gcaacctgag cccgagcgtg agcgtggctg ccggccgaac cggccggggg cgggggagaa
      481 tcccgccgcc gcgcaatcct tatcgagtgc cggagtaccg gttatggtac tcgtggaaat
      541 atgtggacca gattcggccc tttctgaagg ttcctctgct ggccaaagga attctgaccg
      601 ccgaagacgc gcggctgtgc atcgagcatg ggatgaatgg aatcatcgtg tccaatcacg
      661 gagggcgctc catggattac ggcccttcca ctttggagac gctgcccgag atcgtcgatg
      721 ccgtgcaagg gcgcatcccc gtgctgatcg acggcggatt ccgccggggc agcgacattt
      781 tgaaagcgct ggcgctggga gcaaaagccg tctgcctggg acgcaccata aggtggggcc
      841 tgggggctta tggcgcgccg ggagtgcagc gggtgctcga aattctccag accgaactgg
      901 tcttggccat ggcggccacc ggcaggccca cgctggattc gatcgacagt caattggtga
      961 ggacgaactt cgtatgagca aggtcagatt tagcaggcgt cagatgatcg ccggattcgg
     1021 atcgctggcc gccgcttctc ctctgctgcg gggccagcag cccctagcgg gagagcctcc
     1081 aggccgtatc acgccgcgcg acgaactggt gaacgttttc gaatgcgaag cggtagcgca
     1141 gcggaagctg gccagcaccg agtattcgtc catctcggga agcgaccgca gggcgtttga
     1201 ccgcatcacc ttccgccccc ggatgatggt gaacgccatg ggcctggacc tgaccaccga
     1261 gctgttcggg gaaaaaatgt ttgctccaat tctcgtgggg ccggcgtcgc accagcagcg
     1321 ataccaccct gagggggagt tggccacggt tcggggcgct gctgcggcga aagccttgat
     1381 ggtggtcagc agccgttcga gcgtaccgct tgacaagatt gccgccgagg caaagacaac
     1441 gctgtggtac caggtctttc ccgagccgga tgtcgccgcc gtccgatcgc gcattcagaa
     1501 tgcggtgaag gcaggctgca aggcaatcgt cattacggta ggcacgccct atcagcctgc
     1561 gggggcgtca ggcgcgccga acccagccaa gctcgcaatc atggctgatc cggccatcaa
     1621 ctggagcgtg atcgatcaat tgcggcaggc gagcagcgcg cctgttgtgc ttaaggggat
     1681 catgagtccg gaggaagcgc agatcgccgt gcagcgcggc gtgcagggta tcgtggtttc
     1741 caaccatggc ggtcgcttca cgcccggctt ggcttcgccg atcgagatgc tcccggccat
     1801 cgccgacgcc gtgggcggaa aagttccact gctgatcgat ggcagcttcc gccgcgggac
     1861 ggatatgctc aaagccttgg ccttgggcgc ccgggcgatt ctgctcggcc ggccccctct
     1921 ttggggtttg gcggcttatg gcgcggaagg cgttcaaacc gtaatggaaa tgttgcaaac
     1981 cgagctggcc cgcaacatgg ccatgtgcgg caagcctact attaaggaaa tcgatcgcag
     2041 cgccgtgaag attcacaggg catagggatt cgtcacggcg ctgcgcgccg cttcccgcgg
     2101 gcgagcgcga gctcagacgg aaaaagaaga acgggacccg tttgccaaat ccacccagcg
     2161 gcccgcgtgc tcccgtcaat cgagatcgat atcgcgaatt ccgggtttcc cagccggctc
     2221 cgctttagcc tgcttcaaaa gctgatcaaa cttcttctcc agcactttgc catgagtctt
     2281 ctccgcctca aactgctggc ggaagatctg atcgcgtttc ctctcctcgc ctttgagttt
     2341 ttcgacggct tctttcaaat cttcaatggg cggagttttc tcaggcggct ggtgagcaat
     2401 gacaagctgg gtttccgcat ctacctgaag gaccgctttg cagcaggggc accgaacttc
     2461 caacaattcc gctgccattc tcttatcata gcgcagggat acaaagagtt gggcggggag
     2521 cgcggtggca gcgccgcgcc accccctacc ggaaggtgag gtgaaacgac ggccaggaag
     2581 tccggtcctg ccagccttcc aggtacggca cagtaatttt gccgcgctcg gcggccttct
     2641 gctcgcaata gcgccgcacc tcctcgggat tccggttcaa aatgcggcag acaaaagaga
     2701 aactgaagca ggaatcgtcg ctgctccgga tccaccgcaa agcgctgctc cttcttcccg
     2761 acgaactgct gctggcgtcc tggatcgctt gcagcaacat cgctccagcc agccggtaaa
     2821 cgcctcgttc cgacattccg ctttttctcc gacgtttcga atgtaattcg gaatggccgc
     2881 gcgaacaata gcgcaggcga ttcattttag gacgcgagag tgaggtacta gggtactggt
     2941 aatgaccgcc tatagtccca ggcgggcgat caattcgccc caagcgggcg ggtttgcagc
     3001 tctcacggaa aaaccgtaga gctttgcggg atctctttgt ctttctaatt cataggaatc
     3061 gcggccgaaa agcccgtcca gaacggcgga aatcgcggga cgcagttcgc ggtctgtgtc
     3121 ctcggtattg ggcgtctcga gccggtcgtt aaaagccagt tccagccgcc cggtttccag
     3181 atgcacgccg ggcggaaact cgggctcccg cagcaggcag cgggatatgg ctaaggcgtc
     3241 gcgaaaaacg gcccacaagc gctgctggtt tgatccctcc agacccttct ttcgaaagta
     3301 aagcacgcct ggagcggccg ttcccatatc aaagctgagg ttgcaaatat gtccgatcaa
     3361 gacgacgccg gcgccccgag ccatgtgcgc ataatcggcc agatcgaccc attgcgcagg
     3421 gtgagccgca tcggcgcgcc agcgcccgaa gatggagagg aagggcgtca gatccagagt
     3481 ttgcggcgca tcactcagga ttttgacaga gaccttttga atctccgtca tactttggcg
     3541 ccggccatgc ggctataaac agtctgggcc atctccacga acagggtcgc ttcctcgacg
     3601 cgccgccggg ttttttccgg tcccagaccc tgatttcctt cctgggcggc gcggaagaag
     3661 ttctcggcga aggtgccgta aaactcaccg gtgtcgaaaa acagctcgcg gaatttctcg
     3721 acggtgttgt agttatccga aagcagcagg cccttctgga acagaagcgc atcggccgcc
     3781 ttcaggtttg cggcccaggt tttggcagcc gcctcgtcgt agcggcccgc atcgagatcc
     3841 agagtggctt cgaagacgag gcggtcagcg tcctcgagca tgaattccga ctgatccacc
     3901 acctcgccgg cgcattcgcc ctgctcggtg gacatgtggt agtcccaagg ctgccggttg
     3961 tctttgtaat acgccggcgc ttcctcataa gagggcagca ctgtcagctc ctccagctct
     4021 tccttgatgc gcgccttgcc caggcggcgg acgaaatcgg aaaaccgctc gctttccacc
     4081 cgttcgcgcg tataaagatc gctcagcttc ttgatcaccg ccggagcgtt ttgggacgga
     4141 accttggcta cggccaagcc atacgcggag gcgttgccct ccatctcgcc gcccagcaag
     4201 acctggaaca cgggcgccac gtggccgccc ttgcgctgca ccgagccgaa gaagccgata
     4261 tcggcgatat gatgctgccc gcaggagttg aagcatccgc tgatcttgac gttgagatcg
     4321 gtgcgcgcgg ccacatcgct caagccattg tggaattttt catgcaagag cgccgccagc
     4381 ccgcgggagg acgcaatgcc gagtttgcag gaatcggttc cgggacaggc cgtcacatcc
     4441 cctaaagtgc ccgcgccgat ctgcgccaga tccagctcgc gcaacccatc gtaaaaatcc
     4501 ggcagatcgc cgctcgcgac ccagcgcacc atgatattct gctcgaccgt agtgcggatg
     4561 gtgtcattca cgaaacggcg gcacaaagca gccaggccgt gcatctggtc cgcactgatg
     4621 tcgccgagcg gcaaaaaaac ggtgaccgtc gtgtaacccg gctggcgctg cgggcgaacg
     4681 ttggtgcgca gccagcggat aaactcgggg ctgcgcccgg aaagatcgag atccgaaggg
     4741 ggacgcagcg gtttttctgc gaagttttca gcggcttcga gataatccgt ccagcgcgga
     4801 tccggcggaa gcttctggcg ctcctccaaa accaggcgga caaactcctc gatgccgagc
     4861 tgtcccacca gaaacttcat gcgggcgcgg ccccggtttt tcttctcacc caggcgggcg
     4921 aacacacggc caatggcctg cgcgagcggc agcatctcag ccgccgggac gaactcgctg
     4981 aagagcttgg cctggtacgg caaaggcccc aggccgccgc cgacgtacac tttaaaaccg
     5041 cgctccagcc ggccctgttt ctcccgcacc tgagcgatgg ctccaatgtc gtgcatcagc
     5101 gccagagcgc aggcctggtc ctcgcagccg gagaaagcga tcttgaactt gcggccgaaa
     5161 ttctgcgcgt ccgggtggcg cagcatgaaa tacgccattg ctctcgcata gggcgtggcg
     5221 tcgaaaactt cttcctggca aacgccggat ttggggcaag cgcagacgtt cctgacggcg
     5281 ttgccgcaag cctcgcgggt ggtgatgccg gcttccgcca agcggcgcat cagattgggc
     5341 gtgtcgttga tgtttacaaa gtgaagctgg acatcctgcc tcgtagtgat atggcaaacg
     5401 ccgtcggcgt attcctcggc caaatcggca atgcggtcca tttggcgcgc ggtcagcatt
     5461 cccagcggaa ttttgatccg ctgcatctgc acgccgggct gccgctggcc gtacactcca
     5521 aaacgcagcc gcagttcgag aaacaccttc tccgagatga gcccctgctg catacggtga
     5581 agctcggtct cataaacgtc gagctcctct ttcaccgctg ggggaaggtg agcccaccgg
     5641 tcccgctcgg tcaatataga agtggccatt ataatcctct aggctatcac cctaatctag
     5701 attgatatta taggctctcg gattcgttaa cggcaaaacg ttaagaaaag tccccagccg
     5761 cgtagccggc ttccccgccc tgaatcgctc ccggaatcca acaactcctc tcttgtcaga
     5821 gcgttatcag ccgggccagc cgcggaggca ggccagcggc gggcctcagc aaaaaagcca
     5881 tctccaaaac gaaaacccct gggcacggtc gatggcgccc gcgccggccg cttgcattgc
     5941 gtgggtgcgt tgcggttccg gcagctcgca aaaacattgc aagattacca gttcggcgga
     6001 agctgtttgc gaagcgcttt tcccggagcc cggccgccac gcgccggcag gcgcacgcgg
     6061 gctgttggcc cgatcgggca gttttccaac tcagcagcgc cttgaagtcg ggcgacgccc
     6121 gctgtcatag tgaaatagat ggagtttggc tacaccagcg aacagcagca gctccggcgg
     6181 atggtgcgcg agtttgccga atcggagatt gcgccgcacg tgagagagtg ggatgaaaac
     6241 caaacgtttc cggccgaagt gatccggcag ctcggcgcgc tcgggttgat ggggacgatt
     6301 ttcccggaag agctcggcgg ggcgggcatg ggctacatcg aatacgcgat cgtgatcgaa
     6361 gagctgtccc gcgtggacgg ctcggtgggc atcatcgtag ccgcgcacac ctctctgtgc
     6421 acaaatcata tttataagat gggcacgccc gagcagcagc gggcgtacgt tccgaaactt
     6481 gcctccggcg aatggctcgg ctgctggtcg ctcacggagc ccgaggccgg ctcggatgca
     6541 gccggcacgc ggactacggc cgcgcgcgaa aacgattgct gggttttaaa cggcgccaag
     6601 accttcacca ccaacgcgca tcatgccgac gtctgcgtgg cgatggcggt gaccgaacgg
     6661 gcgcagggcc accacggcat atcagcattc ataataccta agggaactcc gggctttcgg
     6721 ctgggacgaa aagaaaacaa gcttggcctg cgggccagtg atacgggcga ggtaatcttc
     6781 agcgattgcc gcctgcccgc ctcgcaactg ctggggaaag cgggcgaggg cttcattgac
     6841 tcgctgaaga ttctggacgg cggccgggta tcgatcgccg cactgggcgt cggcatggcg
     6901 caaggcgcct acgaagcggc tctgcgctac tcgaagcagc gccggcaatt cggccgcccg
     6961 atctccgagt ttcaaaccgt gcagaacaag ctcgccgaca tggccacgga gatcgatgcc
     7021 gcgcgcctgc tgacctaccg cgccggatgg atgctcgatc aaggccagcg caacaccaag
     7081 gaagcatcga tggcgaaatt attttcgagc gaagtggccg tccgcgtggc caacgaagcc
     7141 gtccagatcc acggaggcta cggcttcatt aaggactatc cggtggaaaa attttatcgc
     7201 gacgtcaagt tgtgcactat cggcgagggg accagtgaaa tccagcggct ggtgatcgcc
     7261 aggcagttac tgaaagagta gacgcccgga agcgcaacac cggctctggt ggatgcgtcc
     7321 ccatacggat gccgtacata tcccggcaag cagccgtcgg cccggtcctg aaatccggtc
     7381 aaggtcttcg ctgcgcgggg aacgccagtg cggcgcaggg agcgggcgcc cagcctgttg
     7441 ctccctctct ttcggcgggc cttgcagcgc cggcccgtac ggcaaaaggg agtctgcgcc
     7501 gcgcctggcg gcgtggaaaa agcagacgct tccggccgcg attcccgatg gaaattccct
     7561 tttttcgccg acgctgccgg gacgctatga tgaatgggaa gcgggaggag tcgcgactgg
     7621 agtgtaacca gccagacagc cgggaggtcg gcgcgtggat tcagaagatc cgctccggcg
     7681 acgtacgggc gctggcccgg gctctgagtg aagtggagaa cggcggccct cgggcgcaag
     7741 cgctgctcgc gggcctgttt cctttctccg gccgcgcctg gcgagtcggg gtgacgggcg
     7801 cacccggcgc cgggaaaagc tcgctggtgg attgcctcgc ccgcgaattg cgccgcgagc
     7861 agaagacagt gggcatcatc ggtatcgacc cgacgagccc ttataccggc ggcgccattc
     7921 ttggagaccg catccggatg caggcgcatg catcggatcc cggcattttt atccgctcca
     7981 tggccacgcg cggcttttta ggaggcctga cccgcgccac cggcgacgca gtcctggtgc
     8041 tcgatgccgc cgggcgcgac gtcatcctca tcgagacggt gggcgtaggc caggatgagg
     8101 tggatgtggt gaaagtgagc gatgccgtag cgctggtcct ggcgccgggc atgggtgacg
     8161 acgtgcaagc gctgaaggcg ggtgttttgg aaatcgccga tgtgctggtg atcaacaagt
     8221 cagaccggga aggctccgac cggctcgagc aggaactgcg ggcgatgctg gaaatggccc
     8281 ggcggccgga cggctgggtt ccgccgatcg tccgtacagt ggcaacggag agctccggcg
     8341 tttccgaact cctggccgcc ctgcgcacct gccatttccc cggccggcgc gtttccaact
     8401 ggcgcgcgca gttgctcgag atgctgcaat gccggctgat ggacagattg atcgaaagag
     8461 cgctcggcaa tggagaactg gatgcggcgg cggcagccgt cgccagccgc cagcgcgatc
     8521 cttatcggtt cgtcgaagag gtgatagagc gttatgagcg atgtgcggat tgaccacctg
     8581 gggatcgccg tgcgatcttt gaatgcggca ctttccttct atgagcgggc cctgggcctt
     8641 actgcatatg gacaagaggt agtagaacag gagaaggtcc gcgtggcgat gcttcctgcc
     8701 gccggcggcg ggcctcccga acggctccgg ccccggattg aattgctgga agcgaccgcg
     8761 gaagattcgc cggtcgcgcg ttttatcgcc aagcggggcg aaggtttgca tcacctggcg
     8821 ctgcgcgttc ccaatctgga tgcggccgtg gaccggcttc gcacagccgg ggcgcgcctg
     8881 gtcagccaaa gcgttcaggc cggggcgggg gggcatcgct acatttttgt acatcccagc
     8941 tccacgggcg gagtgctgct cgagctgatc gagggagatt aatgacggca agaatcggag
     9001 tgattggcgg aagcggattc tattccatgg agggcttcgc cgctgcgaag gaagaggcgc
     9061 tggaaacccc ttggggcaag ccttcggaca attatattct aggggagtta agcggccggc
     9121 ccgtagcatt cctctcacgc cacggaagag gccatcgact gtcgccttcc gaaataaatt
     9181 accgggccaa catctacggc atgaaaatgc tgggcgtaga acgcattctc tcatttagcg
     9241 ctgtggggtc tctgaaggaa aatctgggcc cgcgcgattt tgtgattccg gaacagtttt
     9301 ttgaccggac caaggaccgc ccttccacat tcttcggcga ggggttggtc gcccacatca
     9361 gcttcgccga ccccatctgc ccgcaattgg ccggcgtcgc ggaagaagct tgccgcgcgg
     9421 cgggcgtgcg agtgcagcgc gggggcgcct atgtctgcat ggaaggcccg gcgttctcga
     9481 ccaaggccga atcgaacctg taccggtcct ggggaatgga tgtcatcgga atgacgaact
     9541 tgcaggaagc gaagctggcg cgcgaggccg agatctgtta tgtgacgatc gcgatggtca
     9601 ccgattacga ctgctggcac ccaggccacg cggccgtcac cgtggaggat attctgggac
     9661 atttggcggc caacgcccac aacgcgcacc gagtgatcgc cgaggcggtt cagcgcatgc
     9721 ccgcggagcg aacgtgccgc tgcggctcag cgctcgctca cgctctcctg actgatcgca
     9781 atgcgatacc gccggcgacg cgccgcaagc tggcgttgct gattgacaaa tatctaaatt
     9841 gacaaaccct cgccggaggc gccggcttga cggcaccagc ggcgccacat ttgcctgtag
     9901 gcttgctcca gctcccgggc aaccagggct gcgccgcaga gcggcgagcg cactaggcgc
     9961 tcgcgcattc cggcgcgcag ctcccgcaga cggccgggat ttgccgacca gtgcaccgcg
    10021 ccagcgatgt actcgtcgat cgatccggtt acaaattcgg aaagcccgat tcgcgagaga
    10081 atactgacgc cgacgcgggc tgcgtgcgtg cgtcccgcca gagcgactac cggcacgccc
    10141 atccacagag cttcgcacgt cgtaacggtt ccgttatagg ggaaggtgtc cagcgccagg
    10201 tcaacgcggc cgtactcggc cagatgggac gcagcgttgg cgataactcc ggccagctcg
    10261 atgcggcccg gctcgacgcc gtgggcggcg aacatccgga gcaactcctg ccgcgtgccc
    10321 tcatccccaa gagccttggc tttcagaagc aggcggcaat tcgaagattg ccgcagaacg
    10381 gctgcccaca cagctaccac gtcggcagta attttcgagc ggttgttaaa cgagccgaag
    10441 gtgaacaccc cgctgcggta aaacggctgg tctgcaacgg gcggagcatc tgacagaggc
    10501 tggtagcaga agaaacccga gggcaggcgg accagctctt cgctgtgaaa gccttcggcg
    10561 ccgaccgggt ccgcccaggc gtccgtaatg cggtagttga tggcggagag gcccgtggta
    10621 ttgggatatc cgatgtaggt gacctgcacg ggggccggcc cggcagcgaa agcccgcagc
    10681 cggcttcccg ccgtatgtcc tgccagatcg acgagtatgt caattccgtc ctcacggacc
    10741 agcctggcca gttgctcatc cgtcagggcg tggacgtcgc gccatgcccc ggcaagcatt
    10801 tggagacttc tcgtcacggc atcgggctgg agcgtgctgc tgtaacagaa gcactcaaat
    10861 tgacggcggt cgtgggcagc cagaagcggc ccgaacaagt gcgccacggg atgcgaacga
    10921 aagtcaggcg aaacgtaacc gatgcgcagg cgccggccgg gatcgggatg gttggcgtgc
    10981 ggcttgcgaa gcggcaagag cgccgctgcc tgctcctttt cccaacgaag atgctccgtg
    11041 tagatctgcg acggatccag atccgaaaaa tagttcaggc agaaaagcaa attggaatgg
    11101 gcgacggcga agcgagggtc gattcgcagc gcctcgcgaa acgccgcggc tgcctcgtcg
    11161 agtcttcctt gctcaagcag aaccacgccc aggccgctgg ccgtctcggc gcgatcgcct
    11221 ttcagccgca gcgcctcacg atagctggcg gcagcttcgc tgagacggcc ttgcgaccgc
    11281 agcgcgacgc cgagattatg gtgggcttcg gcgaaatccg gcttgatgct cagggctcgc
    11341 cgggccagtg cggccgccgc ctcgctttct ccgcgatcgc cgaggaccgc gccgagattg
    11401 agcagcgctt ccacgcaatc cggctttcgg tccagggcct tacgcagcca cccgattccc
    11461 tcctcagacc gccccgtccg attgagtgta tggccaagat taatccaggc gtcgaggcaa
    11521 tccggctgtt gctccagagc ccggcggtaa cacgctatag catcgtccgg acggcccaga
    11581 cggtaatagc tgttgcccag attcaccaag gcagcggcgt cagcgggccg gcagcggatc
    11641 acagcctcga aacactcggc ggcctgcgcc gcccggcctt gcgcctgaaa tatcttcccc
    11701 aggttgtcgt aatagacggg ttccgagggt ttttgccgaa tcgcttcgcg gatcaggccg
    11761 atcgctccct gaaaatcgcc ggtctgaaac gtcacgaggc cgagcaggtg cagcgcatca
    11821 ctgtcattgg gattgcgagc cagaatgcga tgataaatcc tttcagcatc ccggagccgg
    11881 ccggcccgat ggtgctcgat cgcccgcgcc agagaagacg ccgcttgcgc catatgtatt
    11941 ttcatcggcc agcggagagc ctcgctacat gcctcgatgt ccgattcgcc cacccgccaa
    12001 atttgtaatg cctttggggc ccaaagaaag ccgcggcggc cggttctggc ggcgcccatc
    12061 ggcgcgcgca ggcctgtttt tacgcggcgc tccttccacg agaggcaacc actgtacgct
    12121 cggatacgaa aaggcgggac tcctggttta ccggccaagg cagaagaatt agagtaaggc
    12181 tgtagattat ttcgccaggc gctcgatccg aactgcggcg gatgaggctg gtggtgatta
    12241 tgcgtcggca gacaggccgc ggtgaccgat gagggctgtg catattgccg tgctgctggg
    12301 ggccgctgtg cgcctgttga cggcgcagac agtatccttt atttcccgcg gcgcctttgc
    12361 ggctggggcg tctccttcgg ctctggccgt ggccgatttc aatggcgacg gcttgctgga
    12421 tgtcgccgtg gccaacgggg gattttccag caccgtatcc atcttgctga gcctgggcca
    12481 gggccagttc cggtctccta tgaccttcgc cgccggagcc agtccacgct ccatcctcac
    12541 cggggacttc aacggcgatg gaaagccgga tctggtgaca gcgaacagcg gctccaatga
    12601 cgtttcggtg ctgctgggct cgggcgcagg aatattcttg ccgcctttgc gttttctggc
    12661 cggcgagggc ccccgttttg tggccgccgg cgacttcaac cgggacggca agccagatct
    12721 ggtggtggcg aacgccaatt ccaacaacgt ttccttgctc ctaggcttgg ggcggggcct
    12781 ctttctccct cccatcaact ttgccgtggg cagaagcccg agttccctcg ttgtgggcga
    12841 cttcaaccgc gatggaaagc tcgatctggc ggtggcaaac tccgggtcaa acgacgtttc
    12901 catactgctc gggagcggag acggcaggtt tctgaccgct ttgaattttc cagcggggag
    12961 cggccctgtt tcgattgccg ccggcgattt caacggcgat gggataccgg atctcgcggt
    13021 ggccgacggc ggctttgccg acaacgtttc tgtgttacaa ggtattggca atggggtgtt
    13081 tcaggcggcg cgaaatttcc gggccggcgg cggtctcgcg ttcatcacaa ccggcgacgt
    13141 caatcaagac ggcaagccgg acctcattgt cgccaacggc ggctttggca acaccgtctc
    13201 gatcctgctc ggcgccggct tcgaaaactt tttgcccccg atcagcatcg ccgccggaag
    13261 cagcccggca ggaattgcgc tcggagatgt gaatggcgac aggatgccgg atttgctggt
    13321 cgcgaacagc aatgcgggtc aggtttcggt tctgttgaac agctctttgg gtgtcgcacc
    13381 ttctctccac gccatttcag ccgccagcgg cgcgcctgtg gtggcccctg aatccatcgc
    13441 ctccgtgttt ggcccttctc tagccggcat tacgcaatcg gccgccgccg agccgctccc
    13501 aaaaactctc gggggcatca ccgtggaggt gcaagacagc agaggaacca ggaggccggc
    13561 tcctctcttt tttgtctctc cgaaccaaat taatttcgag attccggctg atctcgcccc
    13621 aggagaggcg gcgatcagca ttctcaccga caccggcgtc tccttcagcg ccacggccca
    13681 ggtgaacctc gtcgcgcccg ggctgtttac cgccgatggg acgggaaacg gcgtggcggc
    13741 ggccgtggta agccgttcac atttggcgga tcctaccgaa gagtttcttc cggcctttca
    13801 atgcgtggat gggcaatgcg aaggcgttcc gatcgagctg agccctgata cggatgtatt
    13861 tttgagcttg ttcggaacag ggcttcgaaa ccggagctcg ctcagccaag ttactgtcgc
    13921 aatcaatggc gtcccggcgc ccgtgttgta tgccggacct caaggctcgt tcaccggcct
    13981 cgaccaggtg aacgtgcttc tgcctcaatc gctgcgaggc agcggcaacg cggacgtcat
    14041 cctgaccgtt gacggccaaa cggcaaatcc tgtgcgtatc aatatcaagt aaacggatcc
    14101 ctcacggcct ggcgcccatt tccgccttac tcatctcaac gacatcatcg cggaattgcg
    14161 gccaggtctc gggagggcgg cggggcgtgg ggatctccgc cgcagcagaa aactcgacaa
    14221 acactcagcg ctttttgggc tcagtgggct atttttctgt aattccgaac gaagcgagga
    14281 atcggggttg accaggccgg agaggaactc tcctttggag ggagaaacga gaaattcact
    14341 ttggtttcgc ccgcaagcgc tgagcacaaa ctagcgccgg cttttccgtg gagcctcacc
    14401 gggctggcgg cgggaaaaaa cccggccccg aactgactga ggccgggtgg tagcgaagaa
    14461 ctggcgctgc tccgatcaac cccgcagcag cgccaggacc gcctggggag cggagttcgc
    14521 ctgcgcgagc gccgcgatac cggactgcaa ggcgatggaa gccttcgtca ggttcgccgc
    14581 ttcagcggcg agatctgcat cgcggatgcg gccttcggcc actgacaggt tgttcacctg
    14641 cgttgaagcc aggttcaccg cgtaattgag ctggttctgg cccttcccga ccgtagcctg
    14701 ggcgcccgcc agcaacgtga ccgccgtcgc cagcgaggat acggcggcct cggcgttggc
    14761 ttgggaactg atgtcggtgg tgctcccgcc gggcagggcc gaagaggtga gcagcgagtt
    14821 gttgcccgca gtgctcacgc cgttgccgct ggctgtattg ccgatgccaa cggtgaacgg
    14881 cgtactcgag ctgctgagga acttgattcc ttccgcgttg ccgctgatct tctccttgac
    14941 cgccacgatc gactgaagag ccggatcatt ggactgctgg agtttcgcgt tgatcgtgtt
    15001 cagggcttga tcgagcgtcg ccgcgttggc attcgcaagc tggatgctca gcgagtgctg
    15061 cgtgccgttg gcggtggtgg tgaatacaag gttttgtacg tcattgccgt agaacagcgg
    15121 cgtgaaggcg gtgggcgcgg tggccgatgt gccgctggtc ccctgcgctc cgcccgaatc
    15181 aaacgtgagc tgggttggct cggccagagt cccggtagtg agggcgccgg cgatggtgcc
    15241 gaacccagta acggcgttcg tggtggtgga agtgagctgg aagtgcgtgc cgttgctgga
    15301 tatgacgttg aggctgccgg tggcggccga ggtgccggcc gttacggtaa ccttcaggcc
    15361 cgcggcttgc aaagtcgggt tggcggtgat ggccgagttc aactgatcgg caaggttctg
    15421 cgtagtgaga cctgcgccgc cagtcgccga aatggtgatg ccgctgcccc cggctccttc
    15481 aatgagcatc gaaatggagc ctgtggctgc taccgtggcg tcggtcgctc cgccggtgat
    15541 cgtcgtgtac gtcacggcgt tggatgcgcc ggtcagagcc gtgcccagtc ccagatagtt
    15601 gttcgtgtcg ccggctacat acacttggaa ggcttcgccc cgcgtattgc tgatgacgag
    15661 cgggttgccc gccgatccgc tcgcgctgta gcccgctgcc gccagagtcg aattcgccgc
    15721 cagagcggcg ttcagattgc tcacgatcgt ggcggctgta tcgctggcgc cgatgttcag
    15781 cgtaacgaat tggggattgg tgaatcccgg tccggcgact ttaagcgtga cagtctcggc
    15841 agccgtcgcg ttggccaagt tccccgctcc ggtgagcgtt tccgccgtcg agagacccgt
    15901 gggatccgag gacgatgtgt aattccccag cagagcgttc gccagccggt cgccggcttc
    15961 tacctggaag gccgtcgtgg ccgaagtaaa gctgagctgc tgcttgccgg tggaatcggt
    16021 gttgataacg gccttgatat tggcgttctt gaaggcggct gcggcctggc tggtcccgtt
    16081 cccggcagcc tggatagcgc tgttgatggc cgtcgccaat gtggtggtat caaccacgcc
    16141 gttcaggttt acggaaacgg cgatcttgtt gacatcgctg aagcccgggc cggcaaagta
    16201 gaagaccgtg ttgccggaga cggcctcgga agcggtgttc gtgctattcg ccagaatggc
    16261 ggcgacattg gtggcggccg aacccgggcc gatgtcagtg gttccagcga ctccgccgag
    16321 agcctgcaat cctttcaatc ccagacttct cgcgtccact gtggaattgc tcagatcgat
    16381 gcccacggtt ccgttgttga tggccacgga actggggtcg cccgcatgtc cgcgcccgcc
    16441 gcctatgaag acggagagcg ccttggcgaa tgtgccgccc tggtcaaggc cgatcgcctg
    16501 cgcctggcga tcgatttcat tgagtacgga ctggaactcc gaactcaatg gtccccggtc
    16561 gccggtgaag gtgccggagg ccgattgcgc cgcgagcgtc cgcgcccgat cgagcaacag
    16621 agagatgttg ttgatgcctc cgtcgatggt ttgcagggtc gacaaaccgt cgccagcgtt
    16681 gcggacgcct tgctgcaata ccgcaatgtc ggagcggaag ctgtttgcga tggccagccc
    16741 ggccgcatcg tcggccgaag aattgatgcg caggccggag gtcaaccgct gaattgtcgt
    16801 ttgctgaaaa ttgctggtga ttcgcaggtt atcctgcgcc agcaaggatg ctacgttggt
    16861 gacaatggaa aacgccatgt gtccgtcctt ggctcggtca gtgcgccggc agtcctttgc
    16921 cggccggcgt ttaggccgta gccgcgcgcc tgttgaaccg cgtccacagt tcttatcggg
    16981 ccacggcagc aaacttcgaa gattttttca agaaggggga gcgaaatccg ccgattcgcg
    17041 gttcagtttc gccaggtgca gatcggcgat gagattctcc tgctcccggc cggcggcttt
    17101 ggaataccgg gccagctttc tggcgcgcaa gatttcgagc aggcgctttt tccgccgcgc
    17161 ttcgatgacg atttcccttt gctcccggcg ccgctgctcc acttgttcgg cccggcctag
    17221 cgtctcttcc ctagaccgct cgagagagcc ggcgtagtca tgggcgtact taagatcggc
    17281 cgccggaaaa gtggagcggg aaaggagagc caggcgggcc gcccggctct gccggcgctt
    17341 ctcttgcgct tcttcgagta aacggacgcg ttgagcggcc aaagcgttaa gccggcttct
//
LOCUS       DASREK010000160        12639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1727108, whole genome shotgun sequence.
ACCESSION   DASREK010000160 DASREK010000000
VERSION     DASREK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12639
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727108"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFA54_08115"
     CDS             <1..320
                     /locus_tag="VFA54_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50809.1"
                     /translation="RISLSMKPPRRPPEKAARPQTMAAAAFRPPNGHAVSKAAGNGLK
                     EKQPRPARAAQEQGPAAKKPAGQVAVPPEKTKPNRPPQVKSKPLTLEEKLALLQSKFR
                     TRV"
     gene            378..1982
                     /gene="pckA"
                     /locus_tag="VFA54_08120"
     CDS             378..1982
                     /gene="pckA"
                     /locus_tag="VFA54_08120"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HZS50810.1"
                     /translation="MQNAGVKPSRFGLIEQGIRNINTAYWNLGVAQLIEKSIARKEGL
                     LAAAGPLVVRTGQFTGRSPQDKFIVRDAAIESNVDWGPVNQPMSPEHFRLLHSKFLAY
                     LQGQDLFVRDCFAGADPAYRLPIRVITQLAWHNLFARQLFIPADGEQTRNHVPEFTIV
                     FAPGFHADPAEDGTRSENCIVVDFCKKTVLIGGTSYAGEMKKSVFSILNYLLPERGVF
                     PMHCSANIGAKGDVALFFGLSGTGKTTLSADPNRRLIGDDEHGWGDFGVFNFEGGCYA
                     KCIHLSQEHEPQIWNAIRFGAVLENVAIDPDLRVLDYDDASITENTRAAYPVDFIDNA
                     VIPGLGGHPSNVVFLTADAFGVLPPIARLTPEQAMYHFLSGYTAKVAGTERGLGKEPA
                     ATFSAGFAAPFLPRPPAVYARMLGDRLRRHKAECWLVNTGWVGGPFGAGRRMSLRHTR
                     AMLHAALEGQLRDVPYAEHPVFGLQVPQVCPNVPAEVLDARGQWADPAAYDKAARELA
                     ARFARNFEKFSGVNETIRQAGPRVSA"
     gene            complement(1988..2332)
                     /locus_tag="VFA54_08125"
     CDS             complement(1988..2332)
                     /locus_tag="VFA54_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50811.1"
                     /translation="MPSGKHNIQLSAVPLSGASLTEALLIGSIGGFVAMVLALRKSRA
                     ARLPMVLWNLLVLVLLISTPLRPSFTFAGRDQLMEGLYAVIAAALALCGSWLQWRVKR
                     PPSRRLSSNARY"
     gene            2477..3814
                     /locus_tag="VFA54_08130"
     CDS             2477..3814
                     /locus_tag="VFA54_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZS50812.1"
                     /translation="MRKTAGLFLILCLLARLAPALEREPAEVFAGRRARLMKSLPNGV
                     TVLFGYTAAEGESLRNPFRQQSDFYYLTGWNEPGAILMLIPPPSERNSPAYSEVSQMP
                     REILFLPARDSVEERWTGVKSGPYDEGVREKAGFQVVLGTEIFEKELASAVTGFGRIY
                     TLKPRPHAADREPVPSRYQTLEKITPLAEIGDAAQAIAALRMVKSPGEIALIQKATDA
                     TVAAHLAAWRRARPGLYEYQVAATMLSILFDRGCERTAYSPIVGAGINSTILHYPENS
                     ARMDSGQVLLMDVGGEYSMYATDITRTIPVGGKFSPRQRELYDVVLGAQKAVLAAIKP
                     GMTLGRSGGNSLHQIAFQYINTHGKDREGNPLGKYFIHGIGHHVGLEVHDPSTPGAEL
                     APGMVITVEPGVYIPEEKIGIRIEDIVLVTDTGAKVLSAALPREAEEIERLMR"
     gene            3823..5496
                     /locus_tag="VFA54_08135"
     CDS             3823..5496
                     /locus_tag="VFA54_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZS50813.1"
                     /translation="MPSGSELFISTLRQFGVTHLFTLVGDHLNDVLRVADRQGLRLID
                     VRHESAAAHMADGWARLTRQPGVSLVTGGPGHTNSITGVATAFATGSPMIAISGMRHS
                     KLGERNAFQELDQISTMRGLTKWAAIPPEPVQLPFYLRRAFTEAINGRPGPVHLSVPF
                     DFFSAEVPATAPPAAPVSLRPAPDRQDVERLLDLLSRSERPVVIAGSGVWWSDACSEL
                     ESFIEKASAPLYTACLGRGAVSDLHPLCFGYGDPSLNRAAKKAFQDADLFLILGKRID
                     YRLGLGGPQVLNPHAQIVQVDIHPQELGLNRKLELPICADIKATLQMASGGLGSLPGT
                     RRQEWLRNLRDGDRKWRQALCAAADAGTPLHPAAVFQALEPGIPDSATLCWDGGDFVH
                     WGRCILRARRPGHWLRLGPLATLGACLPIGLAAKLARPAQPAVVFAGDGSIGFYMAEF
                     DTAVRHNLPVVIILGNDGCWGIEKQLQLGDADARASGGEKTVACGLLRSRYDLVMKAL
                     GGEGEHVERIDQLRPALDRALASGRPYCINIEIRSARSPFSEYQLEMRK"
     gene            complement(5577..6617)
                     /locus_tag="VFA54_08140"
     CDS             complement(5577..6617)
                     /locus_tag="VFA54_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HZS50814.1"
                     /translation="MCSRPAAWARVAGGGGAGGPGIIEVAMAKRAPSRRRPGKAKPQS
                     KGLEAREAYLTSPPEELAEAAAIVEKHRGAVVGCYRDPLGGHPLLLAVLPIDTIQPTP
                     FQRDLSETHHKRLADVIDKTGEFLDPIIAVTAPNDGFWTPNGRHRLEAMRRLGAKAIT
                     ALVVPDRQIAWQILALNVEKAHNLKERSLEVVRIYRGLLEEDGSRPESQFSFYLDDPA
                     LVTLGLCYERKPRFGGGAYHPILRRLESFSESPLRSAMKEHEKRAALVLELEERVAAV
                     VEKLKQRGLTSPYLRSFVVARVNPLRWIKGEPPPLEEVLKTMRDRAARFNVDKVRPQD
                     VANVAGAPEEEG"
     gene            6723..7220
                     /locus_tag="VFA54_08145"
     CDS             6723..7220
                     /locus_tag="VFA54_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50815.1"
                     /translation="MKKWIAMAAVLGFLGAVSAQQRKRQINTGLGFWTTAELNAISAG
                     LKAKEPAGNGNNMYATTGFDFRGNYSAQLTRRDESGVPEIHEGWSDVFIAREGEATLL
                     YGGKIEGGTESRPGEIGGGKLVGAQSKKMAPGDMAFIPAGMPHQTVVEPGRHFTVMIL
                     KVIKP"
     gene            7440..8654
                     /locus_tag="VFA54_08150"
     CDS             7440..8654
                     /locus_tag="VFA54_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZS50816.1"
                     /translation="MRIVDIIPTTVTVPLEAPIRHSNGAHWGRFVRTIVEVKTDDGLS
                     GWGEMGGGGESAEAAFRSLKNYLVGHNPFHLEALRWKISNPTASLYNNRTQLHAAIEF
                     ACLDLIGKKLGVSVSELLGGRLRDQVPFAAYLFSRYPNGSVGGEETPEEMVKQASELA
                     ARCGFQSLKLKCGVFPPDHDIEVYQALASVFPGHRLRLDPNCAWSVEEAIRVGKAIEH
                     LRNDYFEDPTFGLEGMRRVRHFVRIPTATNTVVVNFEQLASCIRTEAVDVILLDTTFW
                     GGLRQAYKAAQVCETFQWGVSVHSSGELGIQLATMLHLGAVLPNLVFAADAHYHHLTD
                     DILAGGKLPYRNGAIDVPTGPGLGVEVNRERLARYAELYKELGGYPYDRDPHRPEWFS
                     VQPESRFATPRR"
     gene            complement(8663..9346)
                     /gene="tsaB"
                     /locus_tag="VFA54_08155"
     CDS             complement(8663..9346)
                     /gene="tsaB"
                     /locus_tag="VFA54_08155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZS50817.1"
                     /translation="MSGPRSVILAIDTTSEFGSLALAHGAEVDEIVPVRSRAGFAHLI
                     FQEIIALLDRHGLNLGDVGGYAAASGPGSFTGVRVGLTAAKGLAEAHGKRVAPVSNLA
                     ALAYSGEGECRAPLIDARRGEIYGAVYDAALRPVVEEMAAPLPRFLEKLGNLPVTFVA
                     SDFTPFRAGISGTRFETARMVTAPRALAGAVAAVAARLFEEGRALLPEQVDANYIRRS
                     DAELLWKET"
     gene            complement(9343..11403)
                     /locus_tag="VFA54_08160"
     CDS             complement(9343..11403)
                     /locus_tag="VFA54_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HZS50818.1"
                     /translation="MPDSRWRIASAFLESRTMIEERSSAARRRCGRMASALAMLASGD
                     HFRHQRTCAGRLVRQTLAAIELRLAARSFTFQIRHRVCLFLTFIFCAGPGQAQRPCRS
                     TPVYQPCDIDFEMTEQEASQHPNPYATVELRAEFRGPKGDTYRLPGFWDGGRKFKIRF
                     APLAEGRWDFRVTSNIERFTGKIESFTATPAVTPGFVYTFNVHHFRYSQPNTGHYWLG
                     DTCYPLAILPPDEFQRLVDARAGQKFNHLRGLLLGDEANAKRAIPTPDQVSPEFFQGL
                     DERVRYMNQKGLTFDVTLAPNAAQLEKLLPNWRQRERFFRYLVARYSAMNITWQGFQT
                     FEDDPESPALLKEFGDLLKQLDIYHHPRSTGAASTSGSLASSGWMDYVTSQTSDPDVF
                     AVEYQLYAAPFVNTGAGAAGRGAAPAQIKVDVDAVRRRMWNAAINGQHLTLDTSALNG
                     GAAEAAATQAQHLRDFFAQTRYFDLEPYFGIEGARALALEEVEYVVFLERPPSSENPV
                     ELTVPKSGYDVSWFNPITGEWVKEHEFKGDRFKVGGPPDPSHDWVLYVRKEGKKQGMM
                     RSYKLESRTPVMQELQVNRNEVPFTIQLPSDSALPPGVPLEFNATLTRPGPATRNMIW
                     MWTGEASSSGFGYRVLGTKQFGEFIVPANIAREYPTALLVRVFGLDGNGKLYAADKVY
                     TLKK"
     gene            complement(11500..12546)
                     /locus_tag="VFA54_08165"
     CDS             complement(11500..12546)
                     /locus_tag="VFA54_08165"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HZS50819.1"
                     /translation="MRQKLLRVSALLLCAMALTAAYYRSKPSTIMADAAKHFLASLTP
                     EQRAKATFPMDSEERLNWHFIPRVRKGLPLREMDGAQKDLAHAFLAAGLSQRGYMKAT
                     TIMSLDQILKDMEQGKGPQRDPEGYFFSVFGEPSENGTWGWRVEGHHVSLNFTVIKGQ
                     MIASSPSFFGANPAEVKQGPRKGLRTLAREEDLARELLASLDAKQKAIAVIDKTAPKD
                     ILTYNKRKVDPENPKGIAGSKLTKKQMEILTSLIEEYADNMPEEIAKTRMDAVHKAGM
                     EKIYFAWAGGPDKGQGHWYQVQGPTFLIEYDNTQNDANHIHSVWRDFHGDFGEDLLAA
                     HYLQTPHRAVAAGD"
BASE COUNT         2461 a         3917 c         3865 g         2396 t
ORIGIN      
        1 agcgaatctc gctgtccatg aagcctcccc gccggcctcc agaaaaggca gcgcggcctc
       61 agaccatggc agcggcggcg tttcgtccgc cgaacgggca tgccgtctcc aaggctgccg
      121 gcaacggcct gaaagagaag cagccgcgtc cggcgcgagc agcgcaggag caagggccgg
      181 cggctaaaaa gccagccggg caagtggctg tcccgccgga gaaaaccaaa ccaaaccggc
      241 cgccgcaggt gaaatccaag ccgcttacgc tcgaagaaaa gctggctctg ctccaatcaa
      301 aattccgcac gcgtgtttag atcaggcgcc ggccggcagc aagtgaaggt gactgcgaaa
      361 caaggggatt ctcgctgatg caaaacgccg gcgtcaagcc cagccgattc ggcctgatcg
      421 agcaagggat tcgaaatatc aataccgcat attggaatct cggcgtcgcg caattgatcg
      481 aaaaatcgat cgcgcgcaaa gagggcctgc tcgcggccgc cggccccctg gtggtccgca
      541 ccggtcagtt caccgggcgc tcgccccagg ataaattcat tgttcgcgac gcagccatag
      601 agtcgaacgt agactggggt cccgtaaacc agcccatgtc gccggagcat ttccgcctgc
      661 tccacagtaa atttctcgcc tacctgcaag ggcaggatct gtttgtccgg gattgctttg
      721 ccggcgccga ccccgcctac cggctgccca tccgcgtgat cacgcagctc gcctggcaca
      781 atctgttcgc ccggcagttg ttcatacccg ctgacggaga gcaaacgcgc aatcatgttc
      841 cggaattcac gatcgtgttt gcaccggggt tccacgccga cccggcggaa gacggaaccc
      901 gttcggaaaa ctgcatcgtc gtggattttt gcaagaagac ggttctcatc ggggggacca
      961 gctacgccgg agagatgaaa aagagcgtct tcagcatctt gaactatttg ctgcccgagc
     1021 gcggcgtttt cccgatgcat tgctccgcca acattggggc taaaggggac gtggccctgt
     1081 ttttcggact ttccgggacc gggaagacta cgctgtccgc cgatcccaac cgccgcctga
     1141 tcggcgatga tgagcatggc tggggcgact tcggcgtttt caattttgaa ggcggatgct
     1201 acgccaagtg catccacctt tcccaggagc acgagccgca gatctggaat gcgatccgct
     1261 tcggcgctgt gctggaaaac gtcgccatcg acccggatct tcgcgttctc gattacgatg
     1321 acgcttccat caccgaaaac acccgggcgg cctatccggt cgactttatc gataacgcgg
     1381 tgatacccgg gttgggcggc cacccctcga acgtggtatt tctcacggcc gacgccttcg
     1441 gcgtgctgcc gccgatcgcg cgccttaccc cggagcaggc gatgtatcac tttttgagtg
     1501 gatatacagc caaggtggcc ggcacggagc gcggcttggg caaggagccc gccgcgacat
     1561 tcagcgccgg gttcgccgcg ccgtttttgc cccggcctcc ggccgtgtat gccaggatgc
     1621 tcggcgacag gctgcgccgg cacaaggctg aatgctggct ggtgaacacc ggttgggtcg
     1681 gcggtccgtt cggcgccgga cgccgtatga gccttcgtca cacgcgagcc atgctgcacg
     1741 ccgccctcga ggggcagctc cgcgacgtgc cttacgccga gcacccagtg ttcggccttc
     1801 aggttcctca agtctgcccc aacgttccgg cggaggttct ggacgcccgc ggccaatggg
     1861 cggacccggc cgcctatgac aaggccgctc gcgaactggc tgcacggttt gcgcggaact
     1921 tcgagaaatt ctcaggggta aacgagacaa tccgccaagc cggacctcgc gtcagcgcgt
     1981 agggctgcta gtagcgcgcg ttggaagaca ggcgccggct gggcggccgc ttcacccgcc
     2041 attgcagcca actcccgcac agcgcgagcg ccgcggcgat caccgcgtag aggccttcca
     2101 tcagttgatc gcggccggcg aaggtgaaag agggccgcaa aggcgtggag atcagcagta
     2161 cgagcaccag aagattccac aacaccatgg gcagccgcgc ggcgcggctt ttccgcaaag
     2221 cgagcaccat cgccacaaac cccccaatcg agccgatcag gagcgcctcg gtcagcgacg
     2281 cgccgctgag gggaactgcg gagagctgaa tattgtgttt tccactaggc attaccagta
     2341 gggctagcag gaaaaggccc aagcagagca cgaaattaaa caagaacgcg aaaaagcgca
     2401 acgccctccc tagagttcgc attttggcgg cggccgttca ccgacggact cgccatttta
     2461 ctgtatactg cgaagcatgc gcaaaaccgc cggcttgttc ctgatccttt gcctgctggc
     2521 cagactggcg ccggccctcg agcgggagcc tgcggaggtc ttcgccggca ggcgcgcaag
     2581 gttgatgaaa agccttccca acggggtcac cgtccttttc ggctatacgg ccgcggaggg
     2641 cgaaagcctg cggaatccgt tccggcagca aagcgatttt tattacttga ccggatggaa
     2701 tgagccgggc gccatcctca tgctcatccc gccgcccagc gaaaggaact cacccgccta
     2761 ttcggaagtc agccaaatgc cccgcgaaat cctgtttctg ccggcgcggg actctgtgga
     2821 agagcgctgg accggcgtta aatccgggcc ctatgacgaa ggcgtgcgcg agaaggcagg
     2881 tttccaggtc gtgctgggca cggagatttt cgagaaagag ctggccagcg ccgtaaccgg
     2941 gtttggccgc atttacacgt tgaagccgcg tccgcacgcc gccgaccggg aacctgtccc
     3001 gagccgctat cagacgctgg agaagattac gccgctcgcc gaaatcggcg acgcggccca
     3061 ggccatcgcc gcgctgcgaa tggtcaagtc gccgggcgag attgcgctaa tacagaaagc
     3121 cacggacgcc acggtcgccg cccatctggc cgcctggagg cgggccaggc caggcctgta
     3181 cgagtatcag gtcgccgcca ctatgctgag tattttgttc gaccgcggct gcgagcgcac
     3241 ggcatatagc ccgatcgtag gcgccggaat caattcgacc atcttgcatt accccgagaa
     3301 ttccgcccgc atggacagcg ggcaggtgct cttgatggac gtgggcggcg aatactcgat
     3361 gtatgccacc gacatcacgc ggacgatccc ggtgggcgga aaattcagcc cgcggcagcg
     3421 cgagctgtat gacgttgttt tgggagcgca aaaggcggtc ctggccgcga tcaagccggg
     3481 catgactctg gggcgcagcg gcggcaacag cctccaccag atcgcttttc aatacatcaa
     3541 tacccacgga aaagacagag aaggcaaccc gctaggcaaa tactttattc acggcatcgg
     3601 ccaccatgtg gggctggaag ttcacgatcc gtccactccc ggcgccgagc tcgcgccggg
     3661 aatggtgatc acggtcgagc cgggcgtcta catacccgag gaaaagattg gaatccgtat
     3721 tgaagacatc gtactggtca cggatacagg cgcgaaagtg ttgagcgccg cactgccccg
     3781 cgaggccgaa gagattgagc gcctcatgcg gtaatcaacg gcatgccgag cgggtctgag
     3841 ctcttcattt ccactctccg gcagttcggc gttactcacc tgtttacgct cgtcggggat
     3901 catctgaacg atgttttgcg cgtggctgac cggcaaggcc tccgcctcat cgatgtgcgt
     3961 catgaatcgg ccgccgcaca tatggcggac ggctgggcca ggctcacgcg ccagccgggg
     4021 gtctcgctcg tcaccggcgg ccccggccat accaattcga ttacgggcgt cgcgacggcg
     4081 tttgcgaccg gaagtcccat gatcgcgatc agcggcatgc gccactcaaa actgggcgag
     4141 cgcaacgcct ttcaagagct cgaccagatc agcacgatgc gcggccttac gaaatgggct
     4201 gccattccgc ccgagccggt tcaactcccc ttttacctcc gtcgcgcgtt caccgaagcc
     4261 atcaacggca ggcccggacc ggtgcacttg agcgttccgt ttgatttttt ttcggccgag
     4321 gttcccgcca cggccccgcc ggcggcgccg gtctctctgc gccccgcccc ggatcgtcag
     4381 gacgtcgaac ggctcctgga tctgctctcg cgatccgagc ggccggtggt gatcgccggc
     4441 agcggcgtct ggtggtccga tgcgtgctcg gaactggaaa gcttcattga gaaagcgtcg
     4501 gcgcctctct acacggcttg cctgggccgc ggcgcggttt cggacttaca cccgctgtgc
     4561 ttcggctatg gcgatccgtc gctgaaccgg gcggccaaga aagcctttca ggatgccgat
     4621 cttttcttga ttctcggtaa aaggatcgat tacaggctcg gtctgggcgg gccgcaggtg
     4681 ttgaacccgc acgcgcaaat cgtacaggtg gatatccacc cgcaagagct ggggttgaac
     4741 cggaagctcg aactgcctat ttgcgcggat atcaaggcta ccctccagat ggcaagcggt
     4801 ggtctcggca gcctgcccgg gacgcggcgt caggagtggc tgaggaacct gagggacggc
     4861 gaccgcaaat ggcggcaagc gctgtgtgca gccgccgatg ccgggacgcc tctgcatccg
     4921 gcagccgttt ttcaagccct ggagccaggc attccggatt cggccactct gtgctgggac
     4981 ggaggcgatt tcgtccactg gggccgctgt attttgcggg cgcggcgccc gggacactgg
     5041 ctgcgcctcg gtccgctggc tacgctgggg gcgtgtctgc cgatcggcct cgccgccaag
     5101 ctcgcccgcc ccgcccagcc ggcggtggtt ttcgccggcg acggctcaat cggattttat
     5161 atggccgagt tcgatacagc ggtgcggcac aacctgcctg tagtcattat ccttggcaac
     5221 gacggttgct ggggcatcga gaaacagctt caacttggag atgccgacgc ccgcgcgtcc
     5281 gggggcgaaa aaaccgtggc ctgcggattg ctccgctcgc gctacgacct ggtcatgaag
     5341 gccctcgggg gcgagggaga acacgtggag cgcatcgatc aattgcgccc ggctctggac
     5401 cgggcgctcg cctccgggcg cccgtattgc atcaacattg aaattcgcag cgcgcgttct
     5461 cctttttccg aataccaatt ggaaatgcga aagtaggggc agcggatgcg ccggtctctt
     5521 ccctggaggc ctgtttcttc cccggagcag gatttccgga ctttttgtcg ccgcaatcac
     5581 ccttcctcct caggcgctcc ggccacgttg gcgacgtcct gcggtctcac tttatccacg
     5641 ttgaaccgcg ccgcccggtc ccgcatcgtt ttcaagactt cctccaacgg cggcggttcg
     5701 cctttgatcc accgcaaagg attgacccgg gccaccacga acgaacgcag atacgggctg
     5761 gtcaagccgc gttgcttgag cttttcgacg accgccgcca ctctctcttc tagttccagc
     5821 accagagcgg cacgtttttc gtgttctttc atcgccgagc gaagtgggct ttccgagaag
     5881 gattcgagac gccgcaggat gggatgataa gctccgccgc caaaccgcgg cttccgctca
     5941 taacagaggc cgagagttac cagggccggg tcatccagat aaaagctgaa ctgggattcg
     6001 ggacgggagc cgtcttcttc taatagcccc cggtaaatgc ggaccacctc gagcgaacgt
     6061 tctttcaagt tatgcgcctt ttcgacgttc agggcgagaa tttgccaggc aatctgccgg
     6121 tcgggcacaa ccagagccgt gatagccttc gctcccagcc gccgcatcgc ttccagccgg
     6181 tggcggccat tgggcgtcca aaagccgtca ttcggcgcgg taacggcgat gattgggtcg
     6241 agaaactccc cggttttgtc gatcacatcg gcaagacgct tgtggtgggt ctccgaaaga
     6301 tcgcgctgga aaggagtagg ctggattgta tcgatcggaa gcacggctag cagcaacgga
     6361 tgtcccccca gcggatcccg atagcagccg acaaccgcgc cccgatgctt ctcgacgatc
     6421 gccgccgcct cagcgagctc ctccggcggc gatgtcagat aagcctcccg cgcttccaag
     6481 cccttggact gcggcttagc cttgccgggc cggcgccgcg atggagcacg cttggccatg
     6541 gcgacttcga taataccagg gccgccggct ccgccgccgc ccgccacccg cgcccaggcc
     6601 gcaggtcgag aacacaaagg gctaaccccc ggagtgaccc tttacatttc ggcaccgggc
     6661 tgcttaaatt gctgcgaccc ggcagaagca tgccggctac tcaaatcagg agagcgcgtc
     6721 tcatgaagaa atggatcgca atggccgcag tgcttggttt tttgggagcg gtttcagcgc
     6781 agcagcgcaa gagacaaata aacacagggc tcggcttctg gaccacggcc gagctgaacg
     6841 ccatttccgc cggactgaaa gccaaagagc ccgccggcaa cggcaataac atgtatgcaa
     6901 ccacgggttt cgattttcgc gggaattatt cagcccagct cacgcgccgt gatgagagcg
     6961 gcgtacccga aattcacgaa ggctggtcgg acgtgtttat cgcccgcgag ggagaggcta
     7021 cgctgctgta cggcggcaag atcgagggcg gaacagaaag ccggccggga gagatcgggg
     7081 gcggcaagct ggtcggcgcg cagagcaaga aaatggcccc gggcgatatg gccttcattc
     7141 cagccggcat gccgcaccaa accgtcgtgg aacccggccg tcatttcaca gtcatgatct
     7201 tgaaagtgat taagccctag cagggcgtcc gctccgaaag cggtctgggt cgcggaagtg
     7261 cagccgcccg gttccagcat gcccgaggcg ggcggttaag ctgcgtttcc cacaaccctc
     7321 tatgggccgc acagccgatt gattgtgcgg tgggttgagc cctcggagcc ggggcatccg
     7381 gtcccaggaa cgcttcgtcc gcgtcaggac gctgtcttac gaagggaaga ttcaagccgg
     7441 tgcgtattgt cgatatcatt ccaactacgg tgacggtgcc gctggaggcc cccatccggc
     7501 actcgaacgg agcacactgg ggccgttttg tccgcaccat tgttgaggtg aaaacggacg
     7561 acggcctgag cggctggggt gagatgggag gcggcggcga atcggccgaa gcggcctttc
     7621 gcagcctcaa aaactacctt gtagggcaca atccttttca cctcgaagct cttcgctgga
     7681 aaatctccaa tcctacggcg tcgctgtaca ataaccggac gcaactgcac gcggcgatcg
     7741 agttcgcctg tctcgatcta atcggcaaaa agctgggtgt aagcgtgagc gaactgctcg
     7801 gaggccgctt gcgagaccaa gtgccgttcg ccgcttacct gttttcccgc tatcccaacg
     7861 gaagcgtagg cggcgaggaa acgccggagg aaatggtcaa gcaggcgagc gaactggcgg
     7921 cgcgctgcgg ctttcaatcc ctcaaattga aatgtggagt ctttccgccg gatcacgaca
     7981 tcgaggtcta tcaggcgctg gcatcggtgt ttcccggcca ccggctgcgc ctcgacccca
     8041 actgtgcctg gagtgtggaa gaggcgattc gcgttggtaa ggcgatcgag cacctgcgca
     8101 atgactattt cgaagaccca acatttggtc tggagggtat gcgccgcgtg cgccactttg
     8161 tccgcatccc caccgccact aacaccgtgg ttgtgaattt cgagcagtta gcgagctgca
     8221 tccgaaccga ggccgtcgat gtgattctgc tggatacgac cttctgggga ggcctgaggc
     8281 aggcttacaa ggcggctcag gtttgtgaaa cgttccagtg gggtgtcagc gtgcattcga
     8341 gcggcgaact gggcatccag ctcgcgacga tgctgcactt gggcgctgtg ctgccaaacc
     8401 tggtcttcgc cgccgatgct cactaccatc acctgacgga cgacatcctg gcgggcggga
     8461 aacttcccta tcggaatggg gcgatcgatg tgcctaccgg tccgggactg ggggtggaag
     8521 tgaatcgcga gcggctggcg cgctatgcgg agctctataa ggagctgggt ggatatccgt
     8581 acgaccggga tccgcaccgt cctgagtggt tcagcgtgca gcccgagagc cgtttcgcca
     8641 cgccccggcg gtagacgcgg ttctaggttt ccttccagag caattcggcg tcggagcgcc
     8701 ggatgtaatt cgcatccact tgctccggca gcagggcccg gccttcctca aaaagacgcg
     8761 cggcaaccgc ggcgacagct ccggccaggg cccgcggcgc cgtgaccatg cgcgcagttt
     8821 cgaaccgggt cccggagata cccgcgcgga acggcgtgaa gtcactagct acaaacgtca
     8881 caggcaggtt acccagcttt tccagaaatc tcggcaacgg agccgccatt tcctcaacga
     8941 ctggccggag cgcggcgtcg taaaccgctc cgtagatctc cccgcgcctg gcgtcaatca
     9001 ggggcgctct gcattctccc tcgccggagt aagcgagcgc cgcgagattc gagaccggcg
     9061 ccacgcgctt cccgtgagcc tcggccagcc ctttggccgc cgtcaggccg acgcgcactc
     9121 cggtaaaaga accagggccg ctggccgccg cgtacccccc gacatccccc aagttcagcc
     9181 cgtggcgatc gagcaaggcg atgatttcct ggaatatgag atgagcgaaa cccgcgcgcg
     9241 agcggaccgg aacgatttca tccacctccg cgccatgcgc gagggccagg cttccaaatt
     9301 cgctggttgt gtcgatcgcc agaattacgc tgcgcgggcc gctcattttt tcagtgtata
     9361 gaccttatcc gccgcgtaaa gcttgccatt cccgtcgagg ccgaagacac gcaccagcag
     9421 agccgtagga tactcgcgcg cgatgttggc gggaacgatg aactcgccga attgtttcgt
     9481 gcccagcacc cgatagccga atccgctgga agaagcttct ccggtccaca tccagatcat
     9541 gttgcgtgta gccggtccag gccgggtaag ggtggcgtta aattccaaag ggacgcccgg
     9601 cggcaatgcg gaatccgaag gcaactggat ggtaaaggga acctcgttcc tgttcacctg
     9661 aagctcttgc atgaccgggg tgcgcgattc cagcttgtac gagcgcatca tcccttgttt
     9721 tttgccctct ttccgcacat acagcaccca gtcgtgggag gggtccggcg gaccgccgac
     9781 cttgaagcga tcgcccttga actcgtgttc cttcacccac tcgcccgtaa tcggattgaa
     9841 ccaactgacg tcgtagcccg acttcgggac cgtaagttcc accggatttt ccgaggacgg
     9901 cggcctctcg agaaaaacaa cgtactctac ttcctcgagc gccagggcgc gggcgccctc
     9961 gattccgaaa tacggctcga gatcgaaata ccgagtctgg gcgaaaaaat cgcgcaggtg
    10021 ctgcgcctgc gtagcggccg cttcggccgc gccgccattc agagccgacg tatcgagcgt
    10081 gaggtgctgg ccattgatgg cagcattcca catgcgcctg cgaaccgcat ccacatcgac
    10141 cttgatttgc gcgggggctg ctccccgtcc ggcggcgcca gcccctgtat ttacgaaagg
    10201 cgccgcgtaa agctggtatt caacggcaaa gacgtccgga tcggaagtct gcgatgtgac
    10261 gtaatccatc caccccgagc tcgccagaga cccggatgtg ctcgccgctc cggtggaacg
    10321 cgggtggtgg taaatgtcga gctgcttgag caaatctcca aattctttga ggagcgcggg
    10381 gctctccggg tcatcttcga aagtctgaaa gccctgccag gtgatattca tcgcggaata
    10441 gcgcgccacc aaataccgga agaagcgttc ccgctggcgc cagttgggca gaagtttttc
    10501 cagttgcgcg gcattgggcg cgagtgtcac gtcaaacgtg aggcctttct ggttcatgta
    10561 gcgcactcgc tcgtccaatc cctggaaaaa ttccggcgaa acctgatcag gtgttggaat
    10621 cgcccgcttt gcgttcgctt catcccccag gagcagcccg cggagatgat taaatttctg
    10681 tcccgcgcgc gcgtccacca ggcgttggaa ctcgtcgggc ggcaggatcg ccagcggata
    10741 gcaggtgtcg ccaagccagt aatgcccggt attgggctgc gagtagcgga agtggtgcac
    10801 gttgaaggtg tagacaaaac cgggcgtcac ggcaggcgtt gctgtaaagc tctcgatttt
    10861 gccggtaaag cgctcgatgt tggatgtaac ccggaaatcc caacggcctt ccgccagggg
    10921 cgcgaagcga attttgaatt tccggccgcc gtcccaaaaa cccggcaggc gataggtgtc
    10981 ccctttcgga ccccgaaatt cggcccgcaa ttcgacagtg gcatagggat tcgggtgctg
    11041 cgaggcttcc tgctcagtca tctcaaaatc gatgtcgcag ggctggtata cgggagtaga
    11101 gcggcaaggc ctctgcgcct ggcccggccc ggcgcagaaa atgaacgtca ggaaaagaca
    11161 cacccgatgc cggatctgaa aagtgaagga tctcgcagct aaacgaagtt ctatcgccgc
    11221 caaggtttgc cggacaagtc tgccggcaca cgttcgttga tgcctgaaat gatccccgct
    11281 ggcgagcata gcgagagcgg atgccatgcg cccgcaacga cgccgcgcgg cgctgcttct
    11341 ctcctcgatc attgtacgac tttcaagaaa agcggaagcg attcgccagc gcgaatccgg
    11401 catgtcgcca ggcgtcttgg agacggcaaa actgagaagg gcgcacgcag cgcggtttcc
    11461 cgtcctgcgc gcgccctgga tgcggcggat cgccttggat cagtcgcctg cggcgaccgc
    11521 gcgatgagga gtttgcaggt agtgcgcggc cagcaagtcc tcgccaaagt cgccgtggaa
    11581 atcgcgccac accgagtgaa tgtggttggc gtcgttctgg gtgttgtcgt attcaatcag
    11641 aaacgtgggg ccctggacct gataccagtg gccctggccc ttatccggcc cgccagccca
    11701 tgcaaaatag atcttttcca tgccggcttt gtgcaccgca tccatgcgcg tcttcgcgat
    11761 ttcctcgggc atattgtcgg cgtattcctc gatcagcgag gtcaggattt ccatctgttt
    11821 cttagtcagc ttggatccgg cgatgccttt gggattctcg ggatccacct tgcgcttgtt
    11881 ataggtcagg atgtctttgg gcgccgtttt gtcgatcaca gcgatcgcct tctgcttggc
    11941 gtcgagggaa gccagcagct cgcgggcgag gtcctcctcg cgcgccaggg ttcgcagccc
    12001 tttcctcggc ccctgcttca cttccgccgg attcgccccg aagaacgatg gcgaagaagc
    12061 aatcatctgt cccttgatca cggtgaaatt cagagacacg tggtgccctt ccacccgcca
    12121 tccccacgta ccgttttccg aaggctcgcc aaagacgcta aagaaatatc cttccgggtc
    12181 ccgctgcggc cctttgccct gctccatatc tttcaggatc tggtccaggc tcatgatggt
    12241 ggtcgccttc atgtacccgc gctggctcag cccggcggcg aggaaagcgt gggccaagtc
    12301 cttttgcgcg ccatccattt cgcgcaaggg aaggcctttg cgcacgcggg ggatgaagtg
    12361 ccagttcagc cgctcctcgc tgtccatggg aaatgtggcc ttggcgcgct gttcgggcgt
    12421 gagcgacgcg aggaagtgct tcgccgcatc cgccatgatc gtgctcggct tgctccgata
    12481 gtaggccgct gtcagcgcca tcgcgcaaag caacagcgca gaaacccgca aaagcttttg
    12541 tcgcatgcat gcctccccta gcggtcgttt tcgaagcgat tatatccaaa ccaagcctcg
    12601 gggggccaga aattcttgga ggatccaaac cggccagat
//
LOCUS       DASREK010000161        10623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_412235, whole genome shotgun sequence.
ACCESSION   DASREK010000161 DASREK010000000
VERSION     DASREK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412235"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VFA54_08170"
     CDS             <1..679
                     /locus_tag="VFA54_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50820.1"
                     /translation="LNVGNTAGVQAIYGDIMNFFGAPAGGSVGGGAACADHTVHGHIT
                     TTPPNSHNGDSHIHHGQHGHTIPGGTECLPPGHHVTVNGHILGLTPESGDNLSGMSIV
                     SPAPEPDFVAALNEDGKMAPAGRGTVVQLFGSAKGLFLDDADSRPALGFTPPLSGSPL
                     YYTDRLPEVRIGGMAAKALFSGLAPGLSGVWQINVRVPEGVPPGPAAVSISYNGQQLN
                     SVGVEVR"
     gene            1170..1625
                     /locus_tag="VFA54_08175"
     CDS             1170..1625
                     /locus_tag="VFA54_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50821.1"
                     /translation="MDQTLKALGDILLQALPTFFLVIALYVYLSRVFFRPLGKVLEAR
                     REAIEGVRSRAEATLRRAEQKTAEYEAALQAARSEIYREQETERRKNQELLAERLKQA
                     RDRAHAQLAEARATIARDVEQAKTSIAGESESLAEQIIHLVLERGVTAR"
     gene            1622..2182
                     /locus_tag="VFA54_08180"
     CDS             1622..2182
                     /locus_tag="VFA54_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HZS50822.1"
                     /translation="MKFLPATVLLLLLAAPAVSAAEEPIEGAAGEPRTLIFQIVNFAI
                     LAGGLAWLAKKYGGPYFASRSESIRNGIAEAENMMAQAEDRARSIERRLAGLNEEIGR
                     LRAAAQNELAAERQRFEQETERAVRRVFAQAEQEIAAVSKAARLELKAYAASLAIGLA
                     EQRIASRITRQTQRELVEDFVRSLDS"
     gene            2186..2764
                     /gene="atpH"
                     /locus_tag="VFA54_08185"
     CDS             2186..2764
                     /gene="atpH"
                     /locus_tag="VFA54_08185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HZS50823.1"
                     /translation="MASALANRYARALVDVVTKPPAGVRGDGWRPPEITPEQITGQLR
                     DFIAVFRTSSELRNVLLSPAVAREKKKIILDEIGRRLGLGRIPQNFLRVVMDHRRLDL
                     IEDIVTGFEALLDERMGILRAGITAAQPVDPDQQSVLAARLGQLTGKQVRLHFSINPR
                     LLGGVVARIDSTIYDGSVLGRLRALERRLAAE"
     gene            2854..4398
                     /gene="atpA"
                     /locus_tag="VFA54_08190"
     CDS             2854..4398
                     /gene="atpA"
                     /locus_tag="VFA54_08190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZS50824.1"
                     /translation="MAQISADEISKIIREQIENYEGAVEVSEVGSIISLGDGIARLYG
                     LEKVMAGELIEFPHGVSGIAMNLEEDQVGAVLLGDYTELKEGDEVRRTRRIMSVPVGE
                     ALIGRVVNSLGQPLDGKGPISTEHYNPIERLAPGVVDRRPVKEPMQTGLKAVDAMIPI
                     GRGQRELIIGDRQTGKTAIALDAIINQKGGDMICVYVAVGQKRSTVAQVVKTLEDYGA
                     MDYSIVVVASASDPAPMQYIAPYAGCAIGEYFRDRGRHALCVYDDLSKHAAAYREISL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLNEANGNGSLTALPIIETQAGDVSAYIPTN
                     VISITDGQIYLEADLFNSNIRPAINVGISVSRVGGNAQIKAMKQIAGTLRLDMAQYRE
                     LAAFAQFGSDLDKASQAQLNRGRHLVEVLKQDQYQPLPVEKQIVIIFAGTNGFLDDLE
                     VEDCRPFEMELYRYLDNARPDLLRKIAAQKEFDKKDELRNEITSVLTEFKQRFLAERQ
                     KKVAAD"
     gene            4499..5365
                     /gene="atpG"
                     /locus_tag="VFA54_08195"
     CDS             4499..5365
                     /gene="atpG"
                     /locus_tag="VFA54_08195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HZS50825.1"
                     /translation="MPSLIDIRRRIRSVRNTQQITKAMKMVSAAKLRRAQDRVIAARP
                     YAALIREVMANLVAHARIFDEEHMPPLLARRPENRILLMLLTGDKGLCGAFNTNLIKA
                     SQSFLAEHSAARVELELIGRKGRDYYRRRQTPVSGEYVNVFAKVEYSVAREIAQKAIQ
                     RFTFQEIDAVYLLYNEFKSVLSQKLVVDRILPLHPPEGREAPVDPIYEEPPEAILRYL
                     LPKYVEVQVYHAMIESAAAEHAARMTAMDSATSNAADMIDSLTLYLNRVRQASITKEI
                     IEVVSGAAAAAQ"
     gene            5374..6822
                     /gene="atpD"
                     /locus_tag="VFA54_08200"
     CDS             5374..6822
                     /gene="atpD"
                     /locus_tag="VFA54_08200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZS50826.1"
                     /translation="MNSNNRHLGKVIQVAGPAVDVQFEEGHLPLIYNAIRITSEGFDV
                     PIPIRILTEVQQHLGEGRVRTISMQPTEGLVRGMKAEDLGHPITVPVGKQTLGRVLNV
                     IGEPVDGMGPVETEHRYPIHRPAPSFEEQSTELQMFETGIKVIDLLEPYLRGGKIGLF
                     GGAGVGKTVVIMELINNIALKHGGVSVFSGVGERTREGNDLWLEMQESGVLDPHDWRK
                     SKVALIYGQMTEPPGARLRVGLTGLTVAEYFRDEEGQDVLLFIDNIFRFTQAGSEVSA
                     LLGRMPSAVGYQPNLATEMGELQERITSTKKGSITSVQAIYVPADDYTDPAPATAFAH
                     LDATTNLSRQISELGIYPAVDPLASTSRILDPRIIGEAHYSTARAVKSILQRYKDLQD
                     IIAILGIDELSEEDKITVARARKIQKFLSQPFFVAAQFTGLEGRYVKLADTIKGFQEI
                     VDGKHDSLPEQAFYMVGTIEEAQERAERLKAA"
     gene            6832..7266
                     /locus_tag="VFA54_08205"
     CDS             6832..7266
                     /locus_tag="VFA54_08205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZS50827.1"
                     /translation="MAHTFTLEVATPERLLIREEVSEAQIPALQGEIGVRPDHAPLIA
                     ELGIGPLSYLAGGRRREMAVSGGIVEVLPDRARVLANAAEHADEIDVQRAEEALRRAN
                     ARLLNPFPGHDVARTLNALKRAQTRLLIARQLHDYPAPHPRH"
     gene            7718..7793
                     /locus_tag="VFA54_08210"
     tRNA            7718..7793
                     /locus_tag="VFA54_08210"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7751..7753,aa:Ile,seq:cat)
     gene            8115..8624
                     /locus_tag="VFA54_08215"
     CDS             8115..8624
                     /locus_tag="VFA54_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HZS50828.1"
                     /translation="MTYNVAELEREISQENKPDDPVVRQLQCQFANALVLYMNYKQYH
                     WQTYGPLFRDLHLMWEEFADQALKTLDPLAERIRMIGQKPAAQLRSLQETASVMSTEG
                     DHNMRRMIEEAHANVLHVIKQMREGARTADQENDPGTVELFSRFVQIYEKQEWFLREL
                     LKKGDQLVS"
     gene            complement(8873..9403)
                     /locus_tag="VFA54_08220"
     CDS             complement(8873..9403)
                     /locus_tag="VFA54_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50829.1"
                     /translation="MSRVSFEHFGGKERRVMWGRKDEEYAADFHLIAKRRLDPFHYQV
                     FSYHYLLEADWKLCCRRLGIDRGQFFHAVYRVQESLGRVFYELEPYGLYPPRDYFATR
                     IGGPSGPHGGASQVPPRKPAFPSGKGQSEPAETAQPMKPGGERCQAGREAGPAAAPHS
                     GDPLWQRTAAAFSFSG"
     gene            9853..10539
                     /locus_tag="VFA54_08225"
     CDS             9853..10539
                     /locus_tag="VFA54_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50830.1"
                     /translation="MTFQEIERRLVHLLNEKVRRGEISQRQLARLTGFTQPHIHNVLK
                     GARAMQTQLADAVLACLDLSVSDLIENGNTGRQEKIPLCDGELGPRRPVPERLDDSTF
                     LLFSEAFVSRFVQPVLVRVALDEDAMSPLIEPGDIVLVDRAEWRRRRPVFDSICVVEV
                     DGQGGLCRCRQVGNSLVLVADNARRAPRFPERVSLATRSILDVVRGTVVWTCREFNSG
                     QAATLHFSLP"
BASE COUNT         2136 a         3220 c         3240 g         2027 t
ORIGIN      
        1 gctgaacgtc ggtaatacgg ccggtgtgca agcgatctat ggcgacatca tgaacttctt
       61 tggcgcgccc gcgggtggtt cggtcggcgg cggcgcggct tgcgcggacc atacggtgca
      121 cggccacatc accaccacgc cccccaacag ccataacgga gacagccata tccatcacgg
      181 gcagcatggc cacacgattc ccggggggac ggaatgtttg ccgccgggcc accatgtcac
      241 cgtgaatggc cacatccttg gtttgacgcc ggaatccgga gacaacttat cgggaatgtc
      301 gatagtttct ccggcgccgg agccggattt cgtggctgct ttgaatgaag acgggaaaat
      361 ggcccctgcc gggcgcggga cagttgtcca attattcggc tccgccaaag gcctctttct
      421 cgatgacgcc gatagcaggc ccgccctcgg tttcaccccg ccgctctccg gctctccctt
      481 gtattacact gaccggctgc ccgaggttcg catcggaggc atggcggcca aggcgctgtt
      541 cagcggcttg gcgcctggcc tgagcggcgt ttggcagatt aacgtgcggg ttccggaagg
      601 cgtgccgccg gggcctgcgg ccgtgagcat ttcctacaac ggccaacagc ttaattccgt
      661 gggagtcgag gttcgctgaa tcgaggttcg cggagcgagc cgccgctcta gaatttccgg
      721 ccggcgatgc gcggtgaacg ggcaggcagc gcgttcgccg cgcgattggc ggcggttgga
      781 gagcggccgc caatccattc acgttcgcca cagaggtctt gggctcggag cacccgggtt
      841 caaaatcaga gacgcggaaa gtccgcggtc gcgggcatcc agggttttcc caaggaaaaa
      901 actttgggct ccggaatccc gggcggccgt gtttctctct gtaatagaat gaagtttttg
      961 gggcaagagc ccgaccccga gtgcgggtcc cgtcgtggac gcgaggagcg cggcgcgaag
     1021 gaaaaaggaa tgcgggatcg gcccctggag aggaaagaat tgcccttccg taatttgact
     1081 tgttcggcgg cggcgtggta cgttctaata cttgtataat tcgctctgcg gcacaccgag
     1141 cagcaacctc ttccttgtag atttttagta tggaccagac gctgaaagcg ctaggtgaca
     1201 ttctgcttca agccctgccc accttcttct tggtgattgc gctttacgtc tatttgagcc
     1261 gcgtgttttt ccggccgctg ggcaaagttt tggaagcccg gcgcgaagcc attgagggag
     1321 tgcgcagccg ggcggaagca acactgcggc gggcggaaca gaagacggcg gaatacgaag
     1381 cggcgctgca agcggcgcgc tcggagattt accgtgaaca ggagaccgaa cgccggaaaa
     1441 accaggaact gctggcggaa cggctgaaac aggctcgtga tcgcgcgcac gcgcaactgg
     1501 ccgaagcgcg cgcaacaatt gcgcgggatg tcgagcaggc caagacttcc attgccgggg
     1561 agagcgaaag tctggcggag caaatcattc atttggtgct cgaaagggga gtcaccgccc
     1621 gatgaagttt ctcccagcga cagtcctcct gctcctgctg gcggcgccgg cggtgagcgc
     1681 tgctgaagag ccaatcgagg gcgcagccgg cgagccgcga accctgattt ttcaaatcgt
     1741 caatttcgcc attcttgccg gcggcctggc ttggctggcc aaaaaatatg gaggcccgta
     1801 ttttgcctcg cgctccgagt ccattcgcaa cggcatcgcg gaggcggaaa acatgatggc
     1861 ccaagcggag gaccgagccc ggtcaatcga gcggcgcctg gccggtctga atgaggagat
     1921 cggccggctg cgcgccgcgg cccagaacga actggccgcc gagcggcagc gatttgagca
     1981 agagacggag cgcgcggtgc ggcgcgtctt tgcacaggcc gagcaggaga ttgcggccgt
     2041 ctccaaagca gcgcgcctgg agctgaaggc ctatgccgct tcgctggcca tcggtctggc
     2101 cgagcagcgg atcgccagcc gcattacccg gcaaacgcag cgggaactgg tggaggattt
     2161 cgtcaggagt ctcgattcgt aaaccatggc cagcgcatta gcaaaccggt acgcccgtgc
     2221 cctggtggac gtggtgacga agccgccggc cggcgtccgg ggcgacggat ggcgcccccc
     2281 agagatcaca ccggaacaga tcaccggcca gcttagagac ttcattgcag ttttccggac
     2341 gtcttccgag ctgcgcaatg tgctgctgtc gcccgccgtg gccagagaaa agaagaaaat
     2401 tattctggat gaaatcggcc gccggcttgg cctcgggcgc attccgcaga attttttgcg
     2461 cgtggtcatg gatcatcgcc ggctggacct catcgaagat atcgtcactg gatttgaggc
     2521 tctgctggat gagcggatgg gcattctgcg agcggggatc acagcggccc agcctgtcga
     2581 tccagaccag cagtcggtcc tggcggcccg cctgggccaa ctgaccggca agcaggtgcg
     2641 cttgcatttc tccattaatc cgcgcttgct cggaggcgtt gtggcgcgga ttgattccac
     2701 gatttatgac ggctccgtac tcggccggct gcgcgctctg gaacggcgtc tggcggcaga
     2761 gtagtccccc gctgcgcggc cgggcggaga ctgcgggcct tgccgccagc ccgcgttcgt
     2821 ggacgccgca attttgggag ttgtgaggtt tttatggcgc aaatcagtgc ggatgagatt
     2881 tccaaaatca tccgcgaaca gatcgagaat tatgaggggg cggtcgaggt ttccgaagta
     2941 ggttccatca tctccctcgg cgacggaatc gcccggcttt acggcctgga aaaagttatg
     3001 gccggcgaac tgattgaatt tccgcacggc gtctccggta ttgcgatgaa cctggaggaa
     3061 gaccaggtcg gcgccgtatt gctcggcgat tatacggaat tgaaagaagg agacgaggtc
     3121 cgacgcaccc ggcgcattat gtcggtgccg gtgggggaag ccttgatcgg ccgcgtggtg
     3181 aactccctcg gccagccgct ggacggcaag ggacctattt ccaccgagca ctataacccc
     3241 attgaacgcc tcgcgcccgg cgtcgtcgac cggcgcccgg tgaaagaacc catgcaaacg
     3301 ggcttgaagg ccgtggatgc aatgattccg attggccgcg gccagcgcga gctgatcatc
     3361 ggggaccgcc agaccggaaa gaccgccatc gccttggacg ccatcatcaa ccaaaagggc
     3421 ggggacatga tttgcgtcta tgtggccgtc ggccaaaagc gatcgaccgt cgcccaggtg
     3481 gtgaaaaccc tggaggacta cggcgccatg gattacagca tcgtcgtggt cgcctccgcc
     3541 tccgatccgg cgcctatgca gtacatcgcg ccttatgccg gctgcgccat aggcgaatac
     3601 ttccgcgacc gcgggcgtca tgcgctctgc gtgtacgacg atctttccaa gcacgcggcc
     3661 gcgtatcgcg aaatttccct gctgctccgg cgaccgccgg gccgggaagc ctacccggga
     3721 gacgttttct acctccacag ccgcctgctt gaacgagccg ccaagctgaa tgaggcgaac
     3781 gggaacggct cgctcaccgc tttgcctatt attgagaccc aagcgggcga cgtttcggct
     3841 tacatcccca ccaacgtcat ctcgatcact gacggccaga tttacctgga agcggattta
     3901 ttcaacagca acatccggcc ggccatcaat gtgggcatct cggtgagccg cgtgggcggc
     3961 aatgcgcaaa tcaaggccat gaagcaaatc gccgggacgc tgcgcctgga tatggcccaa
     4021 taccgcgagc tggccgcctt cgcccagttc ggctcggatc tggataaggc ttcgcaggct
     4081 cagcttaacc ggggccggca cctggtcgaa gtcctcaagc aggaccagta tcagccgctc
     4141 ccggtcgaaa agcagatcgt catcattttc gccggcacta atggattcct cgatgatctg
     4201 gaagtggagg actgccgccc gtttgagatg gaactctacc ggtatctgga caacgcgcgg
     4261 cccgatctgc tccgcaagat cgccgcgcaa aaagagttcg ataagaaaga tgagttgcgc
     4321 aacgagatca cgagcgtcct gacggagttc aagcagcgat tcctcgctga aaggcagaag
     4381 aaggtggcgg cggattgagc aacggctcag cttcgcccgc ccgctttccg ggccaaatgg
     4441 aaggtctggg ccaggcggct gcgccgaaag caactaaggg aaagccggca gcgaatttat
     4501 gccaagtctg atcgacatcc ggcggcggat ccgctcggtg cggaacacgc agcagattac
     4561 gaaagccatg aaaatggtct cggcggccaa gctgcgccgt gctcaggacc gggtgatcgc
     4621 cgcgcgcccc tacgccgccc tcattcgcga agtgatggcc aaccttgtag cgcacgcccg
     4681 aattttcgac gaagaacata tgccgccttt gctcgcgcgg cggcctgaaa accgcatcct
     4741 tcttatgctc ttgaccggcg ataaaggcct gtgcggcgcc ttcaacacca acctcatcaa
     4801 agcgtcccag agctttctgg cggaacactc cgcagcacgc gtcgaactcg agttgattgg
     4861 acgcaaaggg cgggattatt accggcggcg ccagacgccc gtctcaggcg aatacgtcaa
     4921 cgttttcgcc aaggtggagt attccgtggc gcgcgagatc gcgcagaagg ccattcagcg
     4981 tttcactttc caggagatcg atgcggttta cctcctctac aacgagttca agtcggtctt
     5041 gtcgcagaag ctcgtggtgg atcgcatcct gccgctgcat ccaccggaag gcagagaggc
     5101 ccccgtggat ccgatttacg aagaaccgcc ggaggcgatt ctgcgctacc tgcttccgaa
     5161 atacgtcgag gtccaggtct accacgctat gattgaaagc gcggcggccg agcatgccgc
     5221 tcgcatgaca gcgatggatt cggccacgtc caatgccgcc gacatgatcg acagcctgac
     5281 gctgtactta aaccgcgtcc ggcaggccag cattacgaaa gaaattatcg aggtggtcag
     5341 cggcgcggcc gctgccgccc agtaggatta ctcatgaatt ccaacaaccg ccatctcgga
     5401 aaagtcattc aggtcgccgg acccgctgtt gatgtgcaat tcgaggaagg gcaccttccc
     5461 ctgatttaca atgcaattcg cattaccagc gaaggcttcg acgtccccat tccgatccgc
     5521 attctgaccg aggtccagca gcatctgggc gaggggcgcg ttcgcacgat ctctatgcag
     5581 ccgacggaag gtttggttcg cggcatgaag gccgaggacc tgggtcatcc tatcactgtt
     5641 ccagtcggca agcagacatt aggccgtgtg ctgaatgtga tcggagagcc ggtggacggc
     5701 atggggccgg tcgaaacgga gcaccgctac ccgattcacc gccctgcgcc gtcgttcgag
     5761 gagcaatcga cggaactcca gatgtttgaa acaggaatta aggtcattga tttgctggag
     5821 ccctacctcc gcggcggcaa aatcgggctg tttgggggag cgggcgtagg gaagaccgta
     5881 gtgattatgg agctgatcaa taatatcgcc ctgaaacacg gcggcgtttc cgttttctcc
     5941 ggcgtcggcg agaggacccg ggaaggcaat gatttgtggc tggaaatgca ggagagcggc
     6001 gtgctcgatc cgcatgactg gcgcaaatcc aaggtcgctt tgatttacgg ccagatgacg
     6061 gagccccccg gggcgcgcct gcgggtgggt cttaccggct tgaccgtcgc cgagtatttc
     6121 cgcgatgaag agggccagga cgttctgctt ttcatcgaca atattttccg gttcacccag
     6181 gcgggatcgg aggtttcggc tctgctgggg cgcatgccgt cggctgtggg ctaccagccg
     6241 aatctggcca cggaaatggg ggaattgcag gagcgcatca cctccaccaa gaagggatcc
     6301 atcacttcgg tgcaggcgat ctacgtgccc gccgacgatt acaccgaccc ggcgccagcc
     6361 acggccttcg cgcacctgga cgcgaccacg aacctctctc ggcaaatttc cgagctgggc
     6421 atttatccgg ccgtcgatcc gctcgcttcc acgtcccgca ttctggatcc gcgcatcatc
     6481 ggcgaggccc attacagcac ggcccgggcc gtgaagagca ttctccagcg gtacaaagat
     6541 cttcaagata tcatcgccat tctgggcatt gacgagcttt ccgaggagga caagataacg
     6601 gtggcccgcg cgcggaagat ccagaagttc ctctctcagc ccttttttgt cgcggcccaa
     6661 ttcaccggcc tggaaggccg ttacgtcaaa ctggcggaca cgatcaaggg cttccaggaa
     6721 atcgtggacg gcaagcacga ttccttgccg gagcaagctt tctacatggt ggggaccatc
     6781 gaagaggcgc aggaacgggc tgagcggctc aaagcggcat aggcgtagac cgtggcgcac
     6841 acgtttacac tggaagtggc aacgccggag cggctcctca tccgcgagga ggtttccgaa
     6901 gcgcagattc ccgcgcttca gggtgaaatc ggcgtgcgcc ctgaccacgc tcctctgatt
     6961 gccgaactcg gcatcggtcc gctgagctac ctagcgggag gccgccgccg cgaaatggcg
     7021 gtgagcgggg gcatcgtgga ggtcctgccc gaccgcgcac gcgtgctggc gaatgccgcc
     7081 gagcatgccg atgaaatcga cgtccagcgg gccgaagagg ctttgcggcg agccaacgcc
     7141 cggctgctca acccttttcc cgggcacgac gtggcgcgga cgctcaatgc cctcaagcgg
     7201 gcgcaaaccc gcctcctgat tgcccggcag ttgcacgact accccgctcc ccacccccgg
     7261 cattgagccg gatgtatcgc acgagcgccg gagccggaca gagcaattcg agccgcgctc
     7321 gttctcttgc tcgcgcacat accttaaagg aagactccaa gccgcgcgtc gcggtcttcg
     7381 ttgagacacc cgcccccgga tccacggcca gcgcagcccg gacggccccg ccggcgcgtt
     7441 tcttcagcag ccccgccggc cagcgcctct ccgtgatgag atcggccgcg acattcagct
     7501 cgaggaccgc cctgcgtgcg ctccggggtg ttgtcagacg ccgccgggac ccgtctccaa
     7561 atttttcgga ttctcagccc ggcccgatcc gcgaagcgct catcgtcacg aaagggaacc
     7621 gccccagctg atggaaggag aaagaagtcg cgtcccggtt ttccgggtac actatcggat
     7681 agaccgccca gggaaaaagc tggcagccgt ccttcttggg cccttagctc agcggttaga
     7741 gcagcggact cataatccgt tggtcgctgg ttcgaatcca gcagggccca ccaaacgccc
     7801 cgaagtcgga ataaacgaac tcgctttcac cggctttggc gccctttcgc tgtaccgcgc
     7861 cttcaccagt ggtttccgcc gcactgggcg gcatgagagc gcggaccaaa cgtgacgccg
     7921 ctgcggggcg tgcgtgaaga ggcggctggc ggcgcgccgt ttaacgggag gccagcccct
     7981 gagagcctgc catgatcctt cggttttggt ccggcgcggc tcgattgtaa gaatcctctc
     8041 gtttcgcccc gctggagctt gattcagcgc cttccccgga acagtaaact aagaagtgac
     8101 gattggaggg tgccatgacc tacaatgtcg ctgaattaga acgcgaaatt tctcaggaga
     8161 acaaaccaga cgacccggta gtcagacaac ttcagtgcca gttcgcgaac gctcttgtcc
     8221 tgtacatgaa ctacaagcag taccattggc aaacttacgg gccgctgttt cgcgaccttc
     8281 acctgatgtg ggaagagttc gccgaccagg cgctgaagac gttggacccg cttgccgagc
     8341 gcattcgcat gatcgggcag aagccggcag ctcagttgcg ctcacttcag gaaaccgcca
     8401 gcgtcatgtc cacggagggg gatcacaaca tgcgccggat gatcgaggag gcgcacgcca
     8461 acgttctgca cgtcatcaag cagatgcgcg agggggcgcg aaccgccgat caagaaaacg
     8521 acccgggaac ggtcgagctg ttctcccgct tcgtgcaaat ctacgagaag caggagtggt
     8581 tcctccgcga actgctaaaa aagggcgatc agcttgtcag ttgagcgaag gcctcaagcc
     8641 ggcagatatc aatcacgcga tcttgggggc gaagccgctg gcctccgccc ccggagcgcc
     8701 cggctctttc cacttcgcaa atcgccatcc cgatatcccg aatccccagg tacggcggga
     8761 tcgaagcgca agatccggct cctgccaggc agcgttcgcc cgtcttaagg gcggcacgca
     8821 agtccgtttc gggcattctt ccccggcatc ccctcccgaa cccgctgcga agctagccgc
     8881 tgaaagaaaa tgcagcagcg gttctttgcc aaaggggatc cccggaatgc ggcgccgcag
     8941 ccggccccgc ttcgcgcccc gcctgacacc gctctccgcc gggcttcatc ggctgggcgg
     9001 tttctgccgg ctcgctttgc cctttgcccg agggaaaggc cggttttcgc ggtgggacct
     9061 gcgaagctcc accgtgcggt ccggaagggc cgccgatgcg cgtcgcgaaa taatcgcgcg
     9121 gcggatacaa gccgtacggc tcgagctcgt aaaacacgcg ccccagcgac tcttgcacgc
     9181 gatagacggc gtgaaagaac tggccccggt cgattcccag ccggcggcag caaagcttcc
     9241 agtccgcctc cagcaaatag tggtagctga atacctggta gtgaaacgga tccagcctgc
     9301 gtttcgcaat caaatgaaaa tctgcggcgt actcttcatc cttgcgcccc cacatgaccc
     9361 gccgctcttt accgccgaag tgttcaaagg aaacccggct catgtacttt tccttctcaa
     9421 cgcatatccg gaaccgcgcg tagcaggcgc gaaatattgc ccttaataca cagggacagg
     9481 gaataatttt tccttttttc tctctttttt gtccttcgcc gccgcatgtc gtgcacgaca
     9541 atctggccag ggaaagaacc gtcgagcggc tccactccat cttgtcatct cctttgttta
     9601 catatcactc tagtgttatt gctcagcatt ttacttaggg taattggaag gcatatggcg
     9661 tcaatagtcc ggctcaaaaa tgcaataaaa atgttcgata atttcccaaa taatatgtca
     9721 taattcatta agattatctg gctcaatgcg atacgcacgg agcgggcaga cgccgaaaac
     9781 gccccgccaa actgaaccgg ccgttgccgg caggatttcc gcaggcccta tatcccatag
     9841 ctatcagggt ctatgacctt tcaggaaatc gagcggcgcc tggtacacct gctcaatgaa
     9901 aaagtgcgcc ggggagagat cagccagcgc cagttagcgc ggttgaccgg atttacgcag
     9961 ccgcacatac ataatgtgct gaaaggcgct cgcgcgatgc agacccagct tgccgacgcg
    10021 gtattggcct gccttgatct ttccgtcagc gatctgatcg agaacggaaa cacaggcagg
    10081 caggagaaaa ttccgctgtg cgacggtgaa ctgggaccgc gaaggcccgt ccctgagcgg
    10141 ctcgacgatt caacgtttct cctgttttcg gaggcgttcg tctcgcgatt cgtgcaaccc
    10201 gttcttgtcc gcgtcgcgct ggacgaggac gctatgtcgc cgctcatcga gcccggcgac
    10261 atcgtgctgg tcgatcgcgc ggaatggcgg cgccgccggc cggtcttcga ttccatttgc
    10321 gtcgttgaag tggatggcca gggcggcctc tgccgttgcc ggcaggtcgg aaactctctt
    10381 gtgcttgtgg cggataacgc ccggcgcgcc cctcgctttc cggagcgcgt ttcgctggcg
    10441 acccggagca ttctggatgt cgtgcggggc acggtcgtgt ggacctgccg ggagtttaac
    10501 tccggccagg ctgcgactct gcatttctcg ctcccctgag cccgcttggg ccggcggctt
    10561 tcgctgtttc gccttccagc gcccgaaggc cgttttctga ttcgaactac aaaaaaggca
    10621 aaa
//
LOCUS       DASREK010000162         4382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2061638, whole genome shotgun sequence.
ACCESSION   DASREK010000162 DASREK010000000
VERSION     DASREK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2061638"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            597..1544
                     /locus_tag="VFA54_08230"
     CDS             597..1544
                     /locus_tag="VFA54_08230"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZS50831.1"
                     /translation="MRPLFLFLAALSLARGQFFSSTPLGLDAYLPVPDDDPLTAEKVA
                     LGRKLFFDKRLSRDQTLSCASCHDPQRAYTDGRTLAEGIEHTKGERNTPTLVNRGYGA
                     VQFWDGRVKLLEEQVLQPIQNPKELGMTLPEVGARLGIDERTLAQSLASYVRTIRSGN
                     SRVDQYLSAYSGLTPQEQLGMRIFRVKGNCVACHVGPNFTDEKFHNTGVSYHNGRFLD
                     EGRFAVTGKPADHGAFKTPTLREVARTAPYMHDGSLETLEDVVEFYDRGGNPNPYRDP
                     EIRPLHLTPEEKQALIAFLKALSGEISEGVGQPESVAAP"
     gene            complement(1609..2085)
                     /locus_tag="VFA54_08235"
     CDS             complement(1609..2085)
                     /locus_tag="VFA54_08235"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HZS50832.1"
                     /translation="MLQEVFCWWFLALAAIPLFGQGPATQFVSAEPTPSVTAKRGATV
                     EIPLKLTIRQGYHINSNTPAEDYLIPTVLSWDSNQALTAKGVTYPKGEAVKYDFSEKP
                     LQVYSGAITLVSRFAVPGDAPLGPAALRGKFRYQACNDKMCFPPRTLPVSISIAVQ"
     gene            complement(2146..2655)
                     /locus_tag="VFA54_08240"
     CDS             complement(2146..2655)
                     /locus_tag="VFA54_08240"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS50833.1"
                     /translation="MNTRFLLALAALVILASACTSAKRPAAAEKEKNYRMAPEFALQD
                     AHGQTVRLSDFKGKVVMVDFWATWCGPCKIEIPWFIEFQRKYKDRGFSVIGISLDEGG
                     WQTVRPFVEEAKMNYPVLIGNDELASKYGGVDVLPTTFIIDKQGRIVATHQGLTSKDD
                     FEDTIKKLL"
     gene            complement(2689..3396)
                     /locus_tag="VFA54_08245"
     CDS             complement(2689..3396)
                     /locus_tag="VFA54_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50834.1"
                     /translation="MTRLSAFSKPRMHAGLPYARGRPYGEFSRRPTPGSRNGLRQLTA
                     SEQVIVNRLLARLPYYRSAIQILAGRDLGEKGRQLIVGAGESGSRMHAASFLRLGRIV
                     VDEVLLKQPRELARILYHEIFHFVWARLGNPTRLSYENMLRREIALGARGELGWPSEA
                     KKLALRQRAVPANCAARFERQRLWSEYVCESFCDSAAWFCLPGWRRHREWTLKPRFRE
                     RRRRWFQMADVLPRLQL"
     gene            3841..>4382
                     /locus_tag="VFA54_08250"
     CDS             3841..>4382
                     /locus_tag="VFA54_08250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS50835.1"
                     /translation="MLKVLLPLAMLAAKSPLQRSIEFALNNPFKGVYQLNAFDVALLI
                     PYFTILVILSFYGLHRYMLVYTYFKYRKNATGDPVKRFEELPTVTIQLPLYNERYVVE
                     QLLESVCHIEYPREKLEIQVLDDSTDETAAIAEAAAHRWSALGHPVTYIHRSDREGFK
                     AGALQNGMRRSSGELIAIFDA"
BASE COUNT          924 a         1328 c         1295 g          835 t
ORIGIN      
        1 agagcagccg gcgcttcctg cgcggaccat tcctcacggc cagcggccgc ctcggtcctc
       61 tgttgtctct caaaagcagc gcctgggccg ggattttccg gcgatgccgc ttggttcgca
      121 gccatatcga ctcccctccc cggctctgtt gcaagcccgg aaccaatgtg aacggagcga
      181 cgccggctgc tccggagatc tgttccgttt tgcacgcttt cgagacggct cgtcatctca
      241 gagggagtcc gcacacccgg ccggaaaacg cacggtcacg ccccgtattg accatcggtg
      301 cgcggtgccc cgctttcgca gcgcgcggcg tccgcctcgg gtctgaagcc agcgggcagc
      361 gcctcttagc gccaagacgg ctgaagtggc gccggggagc ggcctaagac gaggaacctg
      421 cgacgcggcg gctttgtgag caagcctgtg gtgagcgagc cgtgagagag atgagagatg
      481 agagcctagt gacaaacagg tttttgccat agtaaaattg cgccgcgaga tggggtgtga
      541 gctgcctgta tggcattagc cttcgcctgt gtttctgccg gccggcgaaa agcctgatga
      601 gaccgttgtt cttatttctg gcagcgctgt cgctggcgcg gggccaattt ttctcgagca
      661 cgcccctggg gctggacgcc tatttgcccg ttcccgacga cgatcccctc accgccgaaa
      721 aagtagcgct cggccggaag ctgtttttcg acaagcgcct ttcccgcgac caaacgctct
      781 cctgcgcctc ttgccacgat ccgcagcgcg cctataccga tggccgcacg ctggccgagg
      841 gcattgaaca tacgaaaggc gagcgaaata cgccgacgct cgtgaacaga ggctacgggg
      901 cggtgcagtt ctgggatggc cgcgtcaagt tgctcgaaga gcaagtgctc cagccgatcc
      961 agaaccccaa agaactgggc atgacgttgc ctgaagtcgg cgcccgtctc ggaattgatg
     1021 aacgcactct ggcgcaatcg cttgccagct acgtgcgcac gatccgctcc ggaaattcgc
     1081 gtgttgacca atatctgagc gcctactcgg gcttgactcc tcaggagcag ctcggcatgc
     1141 ggatctttcg cgtcaaaggc aactgtgtgg cctgccatgt cggccccaac tttaccgacg
     1201 aaaaatttca caacaccggg gtctcgtatc acaacgggcg gtttctcgat gagggacgct
     1261 ttgccgtgac cggcaagccg gctgatcatg gcgcgtttaa gacgcccacg ctgcgggaag
     1321 tggcccggac ggcgccctac atgcacgacg gcagcctgga aacgctggag gacgtggtgg
     1381 aattttacga ccgcggcgga aaccccaacc cgtatcggga cccggaaatc agacctctgc
     1441 atctgacgcc agaggagaag caggcgctga ttgcattcct aaaagccctg agcggcgaga
     1501 tcagcgaggg ggtgggacag ccggaaagcg tcgcggcgcc ttgaattctc acattgaggc
     1561 gccgtggcga aactggtttt ctccgcggaa cgcaaagcgg gattgcctct actgtacagc
     1621 tatgctgatg ctcacgggca gcgtgcgggg ggggaagcac atcttgtcat tgcacgcctg
     1681 gtaacggaac ttgcccctga gagccgccgg accgagcggc gcatcaccgg gaacggcaaa
     1741 gcgggagaca agcgtaatcg cccccgagta aacctgtagg ggcttctcgg aaaagtcata
     1801 tttcacggcc tcgcctttcg gatacgtcac gcctttggcc gtgagcgcct ggttcgaatc
     1861 ccacgaaaga acagtgggaa tcagataatc ctcggccgga gtattcgaat tgatgtggta
     1921 gccctgccga atcgtaagct tcagcgggat ttcgacagta gcgccgcgct tggcggtaac
     1981 gcttggcgtg ggctcggcag agacgaactg tgttgcgggc ccttgaccga aaagcggaat
     2041 cgcggcgagc gccagaaacc accagcaaaa aacttcttgg agcaccgatg gaacctccct
     2101 gaaatttttg gctcgcccac gtgcatgata tgtagcgctg ccgcgctaca ggagcttctt
     2161 gatcgtatct tcgaaatcat ccttgctcgt aaggccctgg tgggtggcca cgatgcgccc
     2221 ttgcttgtcg atgatgaaag tggtcggcag gacatccacg ccgccgtact tcgaagccag
     2281 ttcatcgttg ccgatcagca cggggtagtt cattttcgct tcttccacaa aggggcgcac
     2341 cgtctgccat ccgccctcgt ccaaagagat accgatcacg gagaaacccc ggtctttgta
     2401 tttgcgctga aattctatga accacgggat ctcaattttg cagggtccac accaagtggc
     2461 ccagaaatcc accatcacga ccttcccctt gaagtccgaa aggcgcactg tctggccgtg
     2521 ggcgtcctgt aaagcgaact ccggggccat tctgtaattc ttctctttct ccgccgcggc
     2581 ggggcgcttg gccgatgtgc acgcactggc cagtatcact agggcggcaa gcgccaacag
     2641 gaaacgcgtg ttcacgaggc cctccggagc cgatgctctc gaaaaccatt ataactgcaa
     2701 gcgcggcagc acgtccgcca tttgaaacca gcgccgccgt cgttcccgga aacggggctt
     2761 cagggtccac tcgcggtgcc ggcgccagcc gggaagacaa aaccacgccg ccgaatcgca
     2821 gaaactctcg cacacatatt ccgaccagag gcgctgccgc tcgaagcggg cggcgcaatt
     2881 cgctgggacc gccctctgcc gcagggccaa ttttttagct tccgacggcc agcctagctc
     2941 gccccgcgcc cctagcgcga tttccctccg cagcatgttt tcgtagctga ggcgcgtcgg
     3001 gttgcccaaa cgagcccaaa cgaaatggaa gatttcgtgg tacaggatgc gcgccagctc
     3061 gcggggctgc ttcagcaata cctcatcgac gacaatccgg cccagcctca gaaaactcgc
     3121 cgcgtgcatg cgggaaccag actcgccagc ccccacaatc aactggcgcc ccttttcgcc
     3181 aagatcgcgg cccgccagaa tctgaatcgc agagcggtaa tacggaagac gcgccagcag
     3241 ccggttcaca ataacttgct cggaagcggt gagctggcgc aggccgtttc tggaaccggg
     3301 cgtggggcgt ctagaaaatt ctccgtacgg ccgcccccgg gcgtacggca ggccggcatg
     3361 cattcgaggc ttcgagaaag cggaaagcct tgtcatggac cgctcacttg acagccggac
     3421 gccggcagcg agcgccggag catggcgatg cgctgccagc cttcgcccga ttccggccaa
     3481 ataagccatg ttaatgtacc acggaaaaaa ccagaagggg agcgccttcg ccgcacggca
     3541 cggctgaggc gggtcgatgt agatagatgc gcgagatcgg gcagcaggcg aaacgaaaag
     3601 gttagatcca tgcaaaaaac ggtctggtac ttgccggctc aattttccgc gactgtacgt
     3661 ccagatacac ggtaaaatag gcttaaggca aaagtagacc gccctggtat tcaaccggcc
     3721 agtggttgag gccatgcagg cggttcaaaa ccagatagcc cgcctccaaa gcaaccggct
     3781 ggaggcacaa gaggcatggc cggagcgggc agcgaaacct ggcatctgag cggcgacgct
     3841 atgttaaaag ttctgcttcc attggctatg ctggcagcaa aatcgccctt gcagcggtcg
     3901 atcgagtttg ccttgaacaa ccctttcaag ggcgtctacc agctcaacgc ctttgacgtg
     3961 gctttgctga tcccctattt caccattctt gtcatcctca gcttttacgg tttgcaccgc
     4021 tacatgctcg tctacacgta tttcaaatac agaaaaaacg ccactggcga ccccgtcaag
     4081 cgctttgagg agctgccgac ggtgacgatc cagttgcccc tgtataatga acgctacgtc
     4141 gtcgagcaat tgctcgagag cgtttgccac atcgaatatc caagggagaa gctggagatt
     4201 caggtgctcg acgattcgac cgacgaaaca gcggcgatcg ccgaggcggc tgcgcatcgc
     4261 tggtccgccc tggggcatcc agttacctac attcatcgga gcgatcgcga gggattcaag
     4321 gcaggcgctt tgcagaacgg catgcggcgc tcctccggcg aactgatcgc gattttcgat
     4381 gc
//
LOCUS       DASREK010000163         5120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_749761, whole genome shotgun sequence.
ACCESSION   DASREK010000163 DASREK010000000
VERSION     DASREK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749761"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFA54_08255"
     CDS             complement(<1..341)
                     /locus_tag="VFA54_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZS50836.1"
                     /translation="MTRRELLFLPAALSGAARAVAASEPQSVSFPLEAIQGVVTPAEL
                     FFVRNHFVEPEISLSSWKLRVEGRVERPFDWTINDLLGAPKKSLEAVLECAGNPAGGQ
                     SVSNGVWEGVPL"
     gene            complement(499..942)
                     /locus_tag="VFA54_08260"
     CDS             complement(499..942)
                     /locus_tag="VFA54_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZS50837.1"
                     /translation="MGRPYVWGLAAFGRVGVQRVLDMLRAELVLAPPTWRRSIAASCG
                     GHGRRRHREAGSESQAETSERGMLMRVGLSHWNWNHGRGTAEAGPDRAATRREALRRL
                     MLFAAGSPALAQSQRSGPRRSARIRFTCRAIAPKSWGLLFAGQMF"
     gene            1243..3195
                     /locus_tag="VFA54_08265"
     CDS             1243..3195
                     /locus_tag="VFA54_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZS50838.1"
                     /translation="MRIAIDTGGTFTDCVYLDGGELRVLKVFSTPASPAQAILRAIKQ
                     IGCAHDIEIRHGTTVATNTLIERTGARVAFVTTAGFEDTISIGRQARPRLYDWFASPD
                     PPLALCHMRFGVAERTICNGSILQRVEKSDLERVASAVRESGAESAAVSLLFSFANPE
                     NERAVAGALARLGIPVSASHEILPEFREYERASTVLVNAYLTPKMGRYLDGLAERLHA
                     GRKGIKLHVMQSSGGIVSASLAARQPVRTILSGPAGGVVGAGALARLSGFQRIIGFDM
                     GGTSTDVALVDGVCRTTNESLVAGLPVSVPMLDIHTVGAGGGSLAHFDSGGALRVGPQ
                     SAGADPGPICYGRGEQPTVTDANLLLGRLDPELFLGGRMRLDEKRTRHFMERARGTLR
                     SADELAAGVVRLAEATMEKAIRVISVERGHDPRDFALVSFGGAGGLHACTLARALRIP
                     TVLLPRLPGALSAVGILISDVIKDFSRTVMLPAGSGALERHFYELERQGRREMRTEGI
                     PAFVERSLDLRYAGQGYELNVPYGRDFVARFHRAHRERYGYADEKRAVEAVNVRVRMI
                     GRAGPAPFRKVRARKGYGRQAAIRRRRVVFDGRAWDAPVYDRALLRPGDAFSGPAIVA
                     EYSATAALPPGCRARVDEWENIIVEI"
     gene            3198..4778
                     /locus_tag="VFA54_08270"
     CDS             3198..4778
                     /locus_tag="VFA54_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZS50839.1"
                     /translation="MPTADPVELAIFHSGFHSIAEEMGGVLRRTAFSPNIKERRDYSC
                     ALFNGRGDVIAMGDHMPVHLGSMPMSVRAAIDTLRLGPGDVAMLNDPYAGGTHLPDIT
                     MVMPVFVKAVKKPAFYVASRAHHADVGGAFAGSMGLCREIYQEGLRIPPIRVSRGGRL
                     DAEVLALILHNVRTPAEREGDLTAQIGACRIGAQRLQEMAAKYGMRRVTRYMDHLLDY
                     SERLVRAELAALPAGEFAAEDFLDDDGFSGEPVRIRATLRLRPQRGQAEVDFTGTSPQ
                     VQGGINAVYAITWSAVFYVFRCLLREEAPATAGLMRPVRVLAPEGSVVNARRPAAVAG
                     GNVETSQRIVDVLLRALAQAAPRRVPAASAGTMSNLTIGGVDPRTGAPFTYYETIAGG
                     MGARPDRDGVPGIHTHMTNSLNTPIEALEYSYPFRVRRYAYRRGSGGNGRYRGGDGLI
                     REIELLTDAQVTLLADRHRFPPYGLAGGQPGACGRASLIRAGGAVEQLKAKGSVAVHA
                     GDIVSIETPGGGGWGEPQ"
BASE COUNT          891 a         1635 c         1634 g          960 t
ORIGIN      
        1 agcggcactc cttcccacac cccgttgctg accgactggc cgcccgcagg attgccggcg
       61 cattccaaaa ccgcttccaa acttttcttg ggcgctccca acagatcgtt gatggtccaa
      121 tcgaaggggc gttccacacg gccctcgact cttagcttcc aactactcag ggaaatttct
      181 ggctccacaa agtggttgcg gacaaaaaac agttcggccg gagtcaccac gccctggatc
      241 gcttccagcg ggaaggagac actctgaggt tcagaggccg ccaccgcccg ggccgcgcca
      301 gaaagcgccg ccggcagaaa gaggagttca cgacgggtca tagcaagcct acagccgtac
      361 cttaacattt cccgcgcggg tcgcgcctcc ggtggagccg gcttctccgg ctgccaccag
      421 gctgattgcc tggcccgtaa cgatctaggc tcgcagaatc gcacccagct tcggagggga
      481 tcgacgccca ttgatccgtc aaaacatctg cccggcgaaa agcaggcccc atgacttcgg
      541 cgctatagct cggcaggtaa atcgtattcg ggcgcttcgc cgcggccctg accgctgact
      601 ctgcgcaagc gccggcgaac cggcggcaaa cagcatcaga cggcgcaaag cttccctgcg
      661 agtggcggcg cggtcgggac ccgcctcggc tgtgccccgc ccatgattcc aattccaatg
      721 actcaaaccg actctcatta acatccccct ttcgctcgtc tccgcctgcg actcagaccc
      781 agcctcccga tgacggcggc gcccatgccc gccgcacgag gctgcgattg atcgacgcca
      841 ggttgggggc gctagcacca gctcggcgcg cagcatgtcc aacacgcgct gtacgcccac
      901 acggccgaaa gccgccaaac cccacacgta gggccggccc atgagcacgg cgccaacagc
      961 ggagaggcgc tggccatcca cagaagctta cgcagggcct tccggcgagt gtcgtaagat
     1021 aggctggcgc cgcgtttcgc ccaactgcgt tcccaacggc tcagacatgt gggattcagg
     1081 ccggtcatag tcgagtgcct ccactgatcc cgcgttgctc gtgcggtcct caatatactg
     1141 aagcggtgac ggtccgagcc aagctataat ttataatctt tattttttca agggcaaaaa
     1201 ttgcgatcat ctccccagcg ctgcgtcgga ggagcgtgcc ctttgaggat tgccattgat
     1261 accggcggca cttttacgga ctgcgtgtac ctcgatggcg gcgagctgcg cgtattgaag
     1321 gtcttttcca ctcccgccag tccagcgcaa gcgattttgc gagcgatcaa acagatcggc
     1381 tgcgcccacg acatcgagat ccggcacggc acgaccgtgg ccaccaacac cttgatcgag
     1441 cgaaccggcg cccgcgtggc tttcgtcacc accgccggat tcgaggatac gatttccatc
     1501 gggcggcagg cgcggcctcg tctctacgac tggtttgcgt ccccggatcc tcctctggcg
     1561 ctctgccaca tgcgcttcgg agtcgccgag cggacgatct gcaacggctc catcttgcag
     1621 cgcgtggaaa agtccgatct ggaaagagtg gcgtccgcag tgcgcgagtc cggagcagag
     1681 tcggcggccg tatcccttct gttttccttt gcgaatcccg agaacgagag agcggtggcc
     1741 ggcgctctcg ccaggctggg catcccggtt tcggcctccc acgagattct ccccgagttc
     1801 cgcgaatacg agcgggcgtc aacggtgctg gtgaacgcct atttgacgcc caagatgggg
     1861 cggtatctgg acggactggc cgagcgcctc cacgcgggcc gcaagggaat caagctgcat
     1921 gtcatgcagt catcgggggg tatcgtttcg gcctcgctgg cggctcgcca gcccgtgcgc
     1981 accattctct ccgggcctgc cggaggagtg gtcggcgccg gcgccctcgc tcgcctctcc
     2041 ggctttcagc gcatcatcgg ttttgatatg ggcggcactt cgaccgacgt ggccctggtg
     2101 gacggcgttt gccgcacgac caacgaatct ctcgtggcgg gcctgcccgt cagcgtgccc
     2161 atgctggaca tccacacggt gggcgctggc ggagggtccc tggcgcactt tgattccggc
     2221 ggggcgctgc gggtggggcc tcaatcggcc ggcgccgatc cgggcccgat ctgttacggc
     2281 cggggcgagc agcccaccgt cacggacgcc aatctgctgc tcggccggct cgatccggaa
     2341 ctatttctgg gcggccggat gaggcttgat gaaaaacgca cccgccattt catggaacgc
     2401 gcccgtggaa cattgcgcag cgctgacgag ctggcggcgg gtgtggtgcg gctggccgaa
     2461 gccacgatgg aaaaggcgat ccgggtgatt tccgtcgagc ggggccatga tccccgcgat
     2521 ttcgctctgg tgagcttcgg tggagcgggc gggttgcatg cctgcacgct ggctcgggcg
     2581 ctgcggattc ccacggttct cttgccgcgg ctgccgggag cgctctcggc ggtgggcatt
     2641 ttgatatccg acgtcattaa agacttttcg cgaacggtaa tgctgcctgc cggctccggc
     2701 gctctcgagc ggcacttcta cgagctggaa cggcaaggcc gccgggaaat gcgcacggag
     2761 ggaattccag cctttgtcga gcgctcgctc gacttgcgct acgccggcca aggctatgaa
     2821 ctcaatgttc cttatgggcg cgattttgtc gcccggttcc atcgggctca ccgcgagcgc
     2881 tacggctacg ctgatgaaaa acgcgctgtc gaggcagtca atgtccgcgt gcgtatgatc
     2941 ggccgcgccg ggccggcgcc cttcagaaag gttcgggccc ggaaggggta tggccggcag
     3001 gccgccatcc ggcggcgtcg agtcgtcttt gacggccgcg cctgggacgc tcccgtctac
     3061 gatcgcgctt tgctgcgccc gggcgatgct ttttcagggc cggcgatcgt ggctgaatac
     3121 agcgccaccg ccgctctgcc gcccggctgc cgggcgcggg tggatgagtg ggaaaacatc
     3181 atcgtggaga tttgatcatg cctactgccg acccggtaga gctggcaatt tttcacagcg
     3241 ggtttcactc gattgcagaa gaaatgggcg gggtgctgcg gcgcaccgcc ttttctccca
     3301 acatcaaaga gcgccgggat tattcctgcg ccctgttcaa tggccgcggc gacgtgatcg
     3361 caatgggcga tcacatgccc gtccacctgg gatccatgcc catgtcggtg cgcgccgcca
     3421 ttgacacctt gcggctgggg cccggcgacg tcgcgatgct gaatgatccc tatgccggcg
     3481 gcacccatct tcccgacata accatggtca tgcccgtctt tgtgaaagct gttaagaaac
     3541 cggctttcta tgttgccagc cgcgcccatc acgccgatgt gggcggggcc ttcgcagggt
     3601 cgatgggtct gtgccgggaa atttaccagg agggccttcg cattccgccc atccgcgtct
     3661 cgcgcggggg ccggctcgat gcggaggtcc tggcgctcat cctacacaac gtgcgcactc
     3721 cagcggagcg ggaaggcgat ttgacggccc agatcggggc gtgccgcatc ggcgcgcagc
     3781 ggctgcaaga aatggcggct aaatacggga tgcggcgcgt aaccaggtac atggatcatc
     3841 tgctggacta ctccgagcgg ttggtccgcg cggagctcgc cgctctgccc gccggcgagt
     3901 ttgcggccga ggatttcctc gacgatgacg gcttcagcgg agaaccggtt agaattcgtg
     3961 caacccttcg tttgcgcccg cagcgcgggc aagccgaggt ggatttcacg ggaacgagcc
     4021 ctcaggtgca aggcggcatc aacgccgttt acgcgatcac ctggtcggcg gtgttttatg
     4081 tgtttcgctg ccttctccgt gaagaggctc ccgctacagc cggcctgatg cggccggtca
     4141 gagtactcgc gcctgaagga agcgtggtga atgcgcggcg gccagcggcc gtcgcgggcg
     4201 gaaacgttga gacctctcag cggatcgtcg atgtactcct cagagccctc gcgcaagccg
     4261 ctcctcgccg cgtaccagcg gcgagcgccg gcacgatgag caatctcacc atcggcggag
     4321 tggacccgcg caccggcgct ccgtttacct attacgaaac catagccggg ggcatggggg
     4381 cgcggccgga tcgcgacgga gtgcccggca tccataccca catgacgaat tcgctgaaca
     4441 ctccgatcga ggcgctcgaa tattcctatc cttttcgggt gcggcgctac gcgtatcgcc
     4501 ggggttcggg cggaaacggc cggtatcgcg gcggggacgg gttgattcgc gaaattgagc
     4561 tgctcaccga cgcccaagtc acgctgctgg ccgaccgcca tcgctttccc ccttacgggc
     4621 tggccggagg acagcctgga gcttgcgggc gggcttcgct gatccgcgcc ggcggcgccg
     4681 tcgaacagct taaggccaag ggcagcgtcg ccgtacacgc gggagatatt gtcagcattg
     4741 agaccccggg aggcgggggc tggggcgagc ctcaatagct atgttcagag agacaggatg
     4801 ttacaccact ggggactgcg gccgatgtat actaccgcca ctgctgttgt gcgatctgga
     4861 ggaggcctgt ggcggtacag gattcgattt ttcggggcga cgcggccaac cttcccgggc
     4921 cggcgcaaag tccacgcgca cttcgcaata ttcgcggccc tgttggccgc cccatttcca
     4981 gaaaactaga gggctacatc gggcgttgca atcgatttgt gaaagggaca gcgaacatga
     5041 aaaagcctag gatcgcaaaa tggatagccg cggtcgttgt agtgctttcg attcccgcct
     5101 gggcgcgcgc ccaggcggtt
//
LOCUS       DASREK010000164        48760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_907319, whole genome shotgun sequence.
ACCESSION   DASREK010000164 DASREK010000000
VERSION     DASREK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 48760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48760
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907319"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VFA54_08275"
     CDS             complement(<1..420)
                     /locus_tag="VFA54_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZS50840.1"
                     /translation="MIVPCIDLMGGKVVQLRQGREKALEAEPPLQMLARLEAFPEIQV
                     IDLDAAMDRGSNDDIVREMASRAIIRAGGGIRTVDRAMDLLDNGVYRVIVGTAAFTAE
                     NVNVPFLEALPAERVMIALDSRAGKIVIKGWKEATRFS"
     gene            complement(417..1193)
                     /gene="hisF"
                     /locus_tag="VFA54_08280"
     CDS             complement(417..1193)
                     /gene="hisF"
                     /locus_tag="VFA54_08280"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZS50841.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGIHFLQLRDAGDPVELASAYNQAGA
                     DELVFLDITASSERRDILIDVVERTARKVFIPLAVGGGVRSVADARRILLAGADKVSV
                     NTAAVERPQLITELSQEFGAQAVVLAIDARRRQDSSGRQRWDVFTYGGRRDTGLDAVD
                     WARRGEEMGAGEILLTSMDCDGTQAGFDCELTRAVSRATSVPVIASGGGGRPEDFVRV
                     FEEGEADAALAASIFHYGTYSIAGLKEVLARRHIPVRIPR"
     gene            complement(1196..1804)
                     /gene="hisH"
                     /locus_tag="VFA54_08285"
     CDS             complement(1196..1804)
                     /gene="hisH"
                     /locus_tag="VFA54_08285"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZS50842.1"
                     /translation="MTVIFDYGAGNLRSVENMLGALGAGYEFARDADGLRRADRIILP
                     GVGHFGQMMRALDRLGVRGALIERLRAGVPFLGICLGMQALFERSAEAPDVPGLGLFS
                     GSVERFAPGIRAPHMGWNKLELCGSPVLLRGLGEHPYVYFAHSYYVPEQAAGSRAAAL
                     VKYHITYAAAVEAGNVFGVQFHPEKSGAAGQRILANFLGGRF"
     gene            complement(1922..2509)
                     /gene="hisB"
                     /locus_tag="VFA54_08290"
     CDS             complement(1922..2509)
                     /gene="hisB"
                     /locus_tag="VFA54_08290"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZS50843.1"
                     /translation="MRAATVKRNTKETQITARLKIDGRGRYNISTGIRFFDHMLELFT
                     KHGGFDLELAARGDLDVDQHHTVEDVGIVLGQCFAKALGDRKGINRAGYFLLPMDETL
                     AVVAVDLGGRPALVYKDRVRVRKVGDLQAELVEDFFGGFVNHAGANLHARVLYGRSSH
                     HKIEAIFKCFGRAMKYACSKDRRLRNQLPSTKGLL"
     gene            complement(2619..3302)
                     /locus_tag="VFA54_08295"
     CDS             complement(2619..3302)
                     /locus_tag="VFA54_08295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZS50844.1"
                     /translation="MSRLLIFDLDGVLVDVSDSYHETIIRTVQHFSGRRVTRDFIQQF
                     KNRGGFNDDWALSTTVIHEFGFPTPYEEVVSYFQSLFLGENGDGRGEALILREKWMAR
                     DGLLERLQKDWRFAIFTGRTRREARLTIDRCAPHLLFEPIVGSTDVANLKPAPDGILK
                     IRSAHPGIDAVYVGDNVDDARSARAAGIPFIGVAAAGNGRRNETIELLRQEGARAIIS
                     DINELEHVL"
     gene            complement(3299..4393)
                     /gene="hisC"
                     /locus_tag="VFA54_08300"
     CDS             complement(3299..4393)
                     /gene="hisC"
                     /locus_tag="VFA54_08300"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZS50845.1"
                     /translation="MQTDLAAATQAPAARRPQPRAAVERMAPYSPPTAGRQGKLRLDF
                     NENTSGCSPRVLQALKRLATRERLAVYPEYGEAREKLAAFFGMRPGELLFTNGTDEAI
                     QVLVNTYVDGGASVLLLAPSYAMYRFYSEVAGARILEIPYRPADLSFPLEELLDSITP
                     ETRAILIANPNNPTGTPVNLDGIRRILGKAAGAAVLIDEAYFEFYGTTALGLIGEFPN
                     LFVSRTFSKVYGMAALRLGCLFSQAENIGWLHKAQSPYSVNALAVVCACEAVEDQIYV
                     RDYVAEALESRRALCQELDKLGIFYYPSAANFVLARFGSRSLEIRDRLRAKGVLVRDR
                     SYEVPGCIRITVGRKAQLRRFLHVLREVLG"
     gene            complement(4393..5658)
                     /gene="hisD"
                     /locus_tag="VFA54_08305"
     CDS             complement(4393..5658)
                     /gene="hisD"
                     /locus_tag="VFA54_08305"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZS50846.1"
                     /translation="MRRSRRLLQAEAVVKPIIEAVRRRGDRALLEYARQLDGFCGKEI
                     RLDPAILDEAALELPAPFRRAVELASGQIWEYCQMQMPRTWHKRVRAGLSVGQIVRPL
                     DSVAAYIPGGRYPLPSTLLMTVIPAQVAGVKTISVASPSRHPLLLGAAGMLGIRNFFQ
                     IGGAQAIAAFAFGTQTVPRANRIVGPGNIYVAAAKKLLAGETGIDFVAGPTEIVIIAS
                     DKKPPARPHWVAADMVAQAEHDADATAIFLTDSMRLAMRVARGVEEQLSRLNTQAVAR
                     KSIASNGAIIVTRSLDEAADIANRFAPEHISIPEASFLSRITNAGSVFVGPWSPEAAG
                     DYASGPNHVLPTGGAAALRGGLSVNDYVKVISVQELGRQGLQYMAEAITTLARAEGLE
                     GHARSVEARLEATGKPRRGSPSGHKRKDS"
     gene            complement(5735..6604)
                     /gene="hisG"
                     /locus_tag="VFA54_08310"
     CDS             complement(5735..6604)
                     /gene="hisG"
                     /locus_tag="VFA54_08310"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZS50847.1"
                     /translation="MSLKLGIPKGSLENATIDLFRRAGYNISPSARSYFPKIDDPEME
                     CMLIRAQEMARYVEDGILDAGLTGKDWIEENNADVMAVADLIYAKQSFGKVRWVLAVP
                     ESSAIRSVRDLEGKIIATELVRTTERYLAANGVRAKVEFSWGATEVKPPVLADAIVEV
                     TETGSSLRANKLKIIDTVTESNTQLIANRAAWQDPWKQKKLGDLKMLLEGAINALGKV
                     GLMMNVSRDRLPAVLAVLPALKRPTISSLSEEGWAAVNTVLDESTVREIIPRLKAAGA
                     EGIVEYPLNKIVM"
     gene            complement(6601..6981)
                     /gene="hisI"
                     /locus_tag="VFA54_08315"
     CDS             complement(6601..6981)
                     /gene="hisI"
                     /locus_tag="VFA54_08315"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZS50848.1"
                     /translation="MELDFTKLDGLVTAVVQDASTGRVLMVGYMNAEAFQRTVATGRV
                     TFFSRSRKKLWTKGETSGHWLEVREISTDCDQDAVLIRADALGPGVCHNGYRSCFYRR
                     LEGGEWRAAEQRTFDPAAVYGGSQ"
     gene            complement(7292..8119)
                     /locus_tag="VFA54_08320"
     CDS             complement(7292..8119)
                     /locus_tag="VFA54_08320"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZS50849.1"
                     /translation="MGHRIRTAVAAGAVVLLGLACLAQTSKAPREKKHLLVIGESAGF
                     THDSISHGMIMLGKLGEESGLFDVTLRTDVQLITKKPLERNAKNLNYFDAIFFYTTGE
                     LPMDASQKADFLSFLRDDGKGFIGAHSATDTFYQWPEYGELIGGYFDNHPWHQQVRIK
                     VEDRAFPATRHFPSTFSINDEIYQFRNFSRDRVRVLLSIDNASIDLHKPGVHRTDGDF
                     PVAWARRFGKGRVFYCSLGHENEAWDRPDMQKMWLEAIRWVLGLTEGDATPRPKPQN"
     gene            8340..9515
                     /locus_tag="VFA54_08325"
     CDS             8340..9515
                     /locus_tag="VFA54_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZS50850.1"
                     /translation="MAAINAGVEGDALKTAAADYQKASGVRMEVVELPYSSLFEKEML
                     DLTSRTGAYDVIMMDDPWFPRLASDDKLAALEPLYKKAGSAGPDADFIASSLALCHEP
                     YPDGRLYALPYVGNANFFFYRKDLFDRYGLTEPRTWDDVLLAAREIRSREKNVYGYVM
                     RAAPGNAVIADFMPVLWAFGGELFSANGEPELNTPEAAAALRFMIELGKFSPPGYVSF
                     NADEVNAYLLQGRAGMCINWPAWLPAMDDPDRSRVIHKIAYAPMPGERVKGRSALGNW
                     LLAIPAGSKNPDAAFAFIHWATQPAQMRRAAEQGNPPTRRSVFRDAGLMRRFRAFPVQ
                     LQALEEGRPRPRDPDWNEIENVFGIYVSQANSGSISVSEALARSQAEIAAIHHRSRK"
     gene            9512..10399
                     /locus_tag="VFA54_08330"
     CDS             9512..10399
                     /locus_tag="VFA54_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HZS50851.1"
                     /translation="MTGASEAKAKRRWREKYSGRLWLAPALIVLGALSLYPLLFALQV
                     SFRTPSGYGFGNFSRLVTDRLFLISLFQTLVYTAGSLAAEFSIGLGLALLANREFRGR
                     GLLRSVLLIPMLLPPVVVAVIWRLILNPEFGVLNGTLRSLGVNTAKLTWAAAENTALP
                     SVMLVDVWEWTPFLFLLLLAGLQSLPVEPFEAASVDGASKWQIFRRLTLPMLKPVIAI
                     AVLLRGMDLLRVFDQVFILTQGGPGFATETVTLYIYRTAFRFYNFGYAVAMSFVLLIA
                     TTVIARSFLRVLGQQSASA"
     gene            10396..11208
                     /locus_tag="VFA54_08335"
     CDS             10396..11208
                     /locus_tag="VFA54_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HZS50852.1"
                     /translation="MKALSKIGGACLLGAAGVTLFPFYWLATLSLKREVDQFAIPPIW
                     IRFHPTLAHYHDIFWVRPFGLYFLNSLIAASVSTLLALGIGTLAAYSLARFPRPEVSF
                     WILSTRMFPPIVSIIPLFLMLRWANLLDSVLALAVLYTAFNLPFVVWMMRGFFEELPR
                     DLEEAAMMDGETRLGALWKVVLPLVRPGLAATAAFCLVIAWNEFLFALVVAQTDAAMT
                     LPVGIASRVTQYEIKWGAMSAAGVVAILPVFLFAAAMQKYLVRGLSLGAVKG"
     gene            11362..12438
                     /locus_tag="VFA54_08340"
     CDS             11362..12438
                     /locus_tag="VFA54_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS50853.1"
                     /translation="MRLQIEKLGKRFGATHALRDFWLDIGDGELLVLLGPSGCGKTTC
                     LRTVAGLEQPDEGRILLGGRDVTYLEPRERDMAMVFQSYALYPHLNVYENIAFPLRVR
                     KRAESEIEKQVKRAAERLGLAGLLKRRPRELSGGQRQRVALARAIVRRPNAFLFDEPL
                     SNLDAKLRVEMRAELKRLQHELGVTSIYVTHDQAEAMTLGRRLAVLKDGVLVQAGEPE
                     EVYLRPANLFVAGFLGSPSMNFLPGRVDAAGRRFVASGMSIALSPEQLDRLPDGAPVT
                     LGVRPENVEVSRQPRQGWLAGRVYVSESMGNENLVVAEAGGRRISCRTIPEMRLDFEA
                     SIWLFFRSDRLHFFDTASGKSLWP"
     gene            12435..13928
                     /locus_tag="VFA54_08345"
     CDS             12435..13928
                     /locus_tag="VFA54_08345"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZS50854.1"
                     /translation="MNAPLLSGRGIRKSFGTNPVLRGVNFDVRTGEVHVLVGANGAGK
                     STLVKILYGALTPDEGALYWKDAPIAAGDLRQTAARGVAYIPQEIEVIPHLSLVENLF
                     LGSPILRPWKSPLDVVDHKAQTAAAAEVLRRVGVVRDPNTPSRDLSVAEQQLLMIGRA
                     LVRKVEMVILDEPTASLTPVEVDKLFTVIGQLRSAGVGLIYISHRLEEIPRIGDRVSV
                     LRGGEMVFTGLASTPKQQVIRQMIGHDVEGAQARAAVAAAPAIELRNGTLGKRLQDVS
                     FSARSGEVLAFAGLIGSGRTSLARSLFGAEPGFSGDLRLFGAPVRFQSPAEAIRHGVA
                     LLTENPKEQGLVLTADVATNISLPSAPLFSQWWGYFDHRKEASVTAEYARRLGLQPPD
                     VHRLVRLLSGGNQQKVVIARWLLNGARVLLFDEPTRGIDIGAKEQVYDWIRRLTAEGK
                     TVLLFSSETEEVLRLADRILVMRSGRITAELSAAEATEEKILRASFD"
     gene            14082..15086
                     /locus_tag="VFA54_08350"
     CDS             14082..15086
                     /locus_tag="VFA54_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS50855.1"
                     /translation="MSNRDQLAVKNKEGIQAAWVRLNAMKLLRRFGPLLGLLILSAAL
                     SVVSPYFLTWDNILNIFRQSAVNGLLSLGQLVVILTAGIDLSVGSILGVCTVVFAMTV
                     KAGAPAVIGFAASLACGLALGLINGFLLTGLKLPHPFVSTLGMMNVARGAAMLLSGGF
                     PIPDLPAGARYPGAASVAGIPIQVIIMLATFGFFGFLLRHTVFGRDIYAIGGNPEASR
                     LAGIAVNARLNAVYGISGLLAGLAALLLAGRMNAGFPLAGTGAELDSIAAVIIGGASF
                     FGGVGTVSGVLIGALIIAVLRNGLNLLNVSTHWQMVVIGIVIIWAVWVDVLRRRGENR
                     "
     gene            15083..16009
                     /locus_tag="VFA54_08355"
     CDS             15083..16009
                     /locus_tag="VFA54_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZS50856.1"
                     /translation="MIRKLTGLVLVLALVLPGCARGTSSGRPHIAVVLKALDSEFWLA
                     VKRGAESAAAGKADLGIVAPEREINIDQQVSMIEDQVSRGAAALVVAPAAAAQVAPAL
                     DKAAARGIPVIIMDTDIHWPKMVTYVGTDNVKGGELAGEYLAKAMPNGGEVALITGVP
                     SDETHELRKRGFLEGIKKSPNLRLVAEQPANSERALGLTVMENILTSQPNLGGVFITN
                     DQMALGALEAVESRHTNKPVKLVSFDAGSEVLKMISEGKVDAVVAQHPFEMGKRSVEA
                     ALAAIAKQKVEPRIDTGTELVTRDNVAAFRAE"
     gene            16098..16934
                     /gene="iolB"
                     /locus_tag="VFA54_08360"
     CDS             16098..16934
                     /gene="iolB"
                     /locus_tag="VFA54_08360"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HZS50857.1"
                     /translation="MMPLLYKPTSGTGEVLCVSQERAGWETVSLRLIRLERDQRYSIS
                     LPGEELALVMLGGRASITAGGYEWSSLGGRESVFSGMPHTLYLPVGTGRVDIRGLTEY
                     CEIAVCGARADRLFPAAVIEPSAVEIEIRGGENATRQINHIMKPDFPADRILVVEVYT
                     PSGNWSSYPPHKHDVHNPPAEVDLDEIYFYKISRPEGYAIQRVYTRDGWRDETMTVRD
                     NEVVLIPEGYHPVVAAHGYDCYYLNVLAGSARSMAASDDPDYAWVRSSWRNKDPRLPL
                     VH"
     gene            17084..17977
                     /locus_tag="VFA54_08365"
     CDS             17084..17977
                     /locus_tag="VFA54_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZS50858.1"
                     /translation="MIRVANAPVSWGIMEVEGWSPPLPPQIFLDELKAAGYDGTELGP
                     YGYLPTEPQALGAELKRRSLTLTSAFVPLRLKEPGTDLSEAEQVAKLLQALGAKYLVL
                     SDALWREREAVAGRVDEGGVRLTDAEWKTLARNIRAVSEVAAWAGLRCVFHHHVGTYV
                     ETPAEVERLLDMTDLGLCLDTGHYVYGGGDPVEAVRKFGKRIEYLHFKDVDPSRLRSA
                     NQARLSFLDGVRSGVFCELGSGAVRFPELITELSRIGYDGWAVVEQDVDASGAPARPG
                     AKTPFESARSSREYLRTKLGI"
     gene            18184..18810
                     /locus_tag="VFA54_08370"
     CDS             18184..18810
                     /locus_tag="VFA54_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZS50859.1"
                     /translation="MKRITALGAISLFAVLSSLHAQTSKLAINGGGGVTLPVGDTGAR
                     LNTGWNITLGAGYNFVPRLGVMGEFTYNRFDINQATLNALSQPDGNLRLWAFTADPIV
                     RLNPGGRLDFYVIGGGGIYHRVVEFTRPTVTTVTVFDPFFGFYNVFVPANEVLSSFAT
                     TKGGLNIGGGIAFRLGSSRVKVYTEARYHHMFTRTATTILPVTFGLRW"
     gene            19123..20229
                     /locus_tag="VFA54_08375"
     CDS             19123..20229
                     /locus_tag="VFA54_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZS50860.1"
                     /translation="MSVLDLLILRIIFIAAVGLTALFIKPFGLQSWWQAGGMGLLMGG
                     AIIFFEIRLRRASLKRLIGAAVGSLFGILAALLMSVVLDLSGAFPYQTLSFLHLGLLL
                     LTSYVGLVVGASKGDMLNLAALGGLFGSEKTANRSSKILDTSVIIDGRIADICETSFL
                     DGVLVIPQFVLRELQLVADSADSLKRNRGRRGLDVLQRIQKMSNIQVQIVEDDFPHIR
                     DVDMKLIELAKLYDGKIVTNDFNLNKVAQLHGVEVLNINELTNALKPIVLPGEVMRVF
                     ILKEGKEYNQGVAYLDDGTMVVVDNAKRMISKTIDISVTSVLQTTAGKMIFGKYDERT
                     QHTPPTQGPERGSGSADRPEAPRGRERSEQVVNH"
     gene            20234..20977
                     /gene="ispD"
                     /locus_tag="VFA54_08380"
     CDS             20234..20977
                     /gene="ispD"
                     /locus_tag="VFA54_08380"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZS50861.1"
                     /translation="MRVAAIIPAAGLGTRMGRGVSAGSPPDKSLPEKSGTSRKQFMLL
                     EGAPILVHTLRKFLACPAVDQIVVALRAEDMDWVREQLRRENPSKSVELVIGGEHRQQ
                     SVRHGLAAVGPGTDLVAVHDAVRPFVSIDLIGKVIAAAEETGAAILGIPPVDTVKQVH
                     RTIIHSTLPREHIVLAQTPQVFRVDLLRRAYEQAEKDGFVGTDEASLVEHLDVRVTVV
                     LGNDRNIKITKPSDMALARLFLQEEMASV"
     gene            21236..23629
                     /locus_tag="VFA54_08385"
     CDS             21236..23629
                     /locus_tag="VFA54_08385"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS50862.1"
                     /translation="MRGNAAARLFLGLVFSAAAWSQGLSTINGTVTDPSGAVIPGARI
                     VVREVETSLSRETISNGDGLYVIGSLRPTRYTLSAEAQGFRQFTQSGIVLEANDTVTI
                     NLKLEVGSTAETVNVEAAATQVDTTTSTLRQVVDSARMIELPLNGRNAAQLTALVAGA
                     VNAPSNNADQGTTKTFPAAVTVSVNGGRQNNIAYNLDGVHSEDIFSNVNQPLPMPDAL
                     QEFSFQTSNFSAEYGQNSSGVVNVVTKSGTNSLHGDVFEFVRNARFNARNFFAARRDQ
                     LKRNQFGGTIGGPILRDRLFFFGGYQGTRIRNTQGGLSAFVPTAADLNGDFSAYLNAS
                     DPSNPLHRAISLKDPATGQPFPGNQIPTSRFDPAALAMLKYLPASASANGLVFYSTPV
                     IQNFDEFLIRLDYSVSASDRLNFRFNKAWYNQPGIFANNNLLTYADHTPDSSYNTAIQ
                     ETHVFSPTLLNDFRFGVTREITSRHPPDNTPNVRDFGVQNIYQTPNKAIESFSVSGYF
                     SFGAFADSIFARATFDWYDTIRWVKGRHNFAIGGAFERARFNQNNHLFQNGTFSFTGD
                     ISGNALADFLLGRLRTFTQGWGSFQRDRNILFSLFIQDTFKVSSRLTLSYGLRFEPSF
                     PWHDLYNQAEAFSPALYYQGVRSQVYTNAYPGEIFTGDPGFPPDGRSNSWNNLAPRLG
                     FAYDPSGNGKTSIRGGAGIFLNSRVPAFSNDAMVQTSPFSPTVSLINPPSFSNPYQGV
                     TNPFPLTFPVPHDFVFPTPVRVYSWDASHYKLQTPTVYNWNLTIERQVRPNWLLRAA"
     gene            23648..24505
                     /locus_tag="VFA54_08390"
     CDS             23648..24505
                     /locus_tag="VFA54_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50863.1"
                     /translation="MNENEQLNPSVYIPGSSLSTDARRPFQPYTSIVQATGSGNAWYH
                     SMQLSLEKRLSRGFTILANYTWSKSLDNMPQGTDVTSPILNAVIGMSPYVPHFKSLDT
                     GPSDFDFNHVFVVSYVWQLPALQNSRRWLRVAAGNWQLTGITSAQTGGPITITAGQDR
                     SQTGIGNDKGQLLAQSVYGSGACGTAAPCVDYLAPTAFGLPPLGTYGNIAKGTLRGPG
                     LFNTDLGVFKRFPINERWSVQFRAEFFNAFNRVNLNNPVTSVSGAGFGSIRGARDPRI
                     GQLALKVFF"
     gene            24657..25136
                     /gene="ispF"
                     /locus_tag="VFA54_08395"
     CDS             24657..25136
                     /gene="ispF"
                     /locus_tag="VFA54_08395"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZS50864.1"
                     /translation="MEFRTGIGYDLHQLVEGRPLILGGVVIPHNKGLSGHSDADVVSH
                     AATDALLGAATLGDIGEHFPPSDPKWKDASSLLFLQRARELLHQSGFEIVHLDIVIVI
                     EQPKILSYRGKIRESIAGALGIDPQAVSLKAKTAEGIGPIGAGQAAEAHAIATVRKA"
     gene            complement(25418..27133)
                     /locus_tag="VFA54_08400"
     CDS             complement(25418..27133)
                     /locus_tag="VFA54_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZS50865.1"
                     /translation="MLVATTMPTGQADAQALRRRTARLAVYRAFLALTYLAAEAFTSR
                     PEFTTPAAAIVLAFVAYSIVLLLYDSHTLAKTFTLPILFLDLLFLISLVFWAITPMSA
                     ALAGAFFAFLVLESWSLHGAREVILLACALALVVYGVRATAGRMPPLIVTVHSVLFLL
                     VVGGSLAFLFSDQQYLVERRILAIANKISGAPEPDTIAAIRDGLRELAAWFRCSHAFL
                     AFWDQRNDHYWLCRYPPPEEGNAAGAPVLEESREWAVFVGEQLTFLAESVAAPDRTEW
                     RAEAARQEPPAAGHPGYDLHMHLVRKFQIYNAVGCGLYHEGKAIGRLLLINRISPMRS
                     LLLRRLRRVAPAFTELVRHLLVINAVEHAAAERERHRIAQDFHDGPLQSVISFQMRVH
                     TIRKLLERDPETAVRELEQLQQVAKRQVAEMRTFVNSMRPVEVDTTSLTAAARRLVDD
                     FQKESGVPVTFISGSEPVRTPGKITVDVLQVIREALHNIHKHAQATHVLFSLEKSDTS
                     LLIGVNDNGRGFRFGGKYSLDELETLRLGPNSIKQRMRALGGDVVLESQPGHGTNLKL
                     KIPLY"
     gene            complement(27181..27618)
                     /locus_tag="VFA54_08405"
     CDS             complement(27181..27618)
                     /locus_tag="VFA54_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50866.1"
                     /translation="MPFCTNCGTPLGDRDFFCGRCGARQPVAGAPSAPSEGGGISART
                     AAMLCYIPVAGWIAAILVLASERFRNAADVRFHAFQGLYLFVAWLLLEWFPWFHLVPM
                     HFFPFRAMLQLLIVFVWIFMLIKASREERYHLPLIGELAERSL"
     gene            27749..28105
                     /locus_tag="VFA54_08410"
     CDS             27749..28105
                     /locus_tag="VFA54_08410"
                     /inference="COORDINATES: protein motif:HMM:NF026924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm8 family immunity protein"
                     /protein_id="HZS50867.1"
                     /translation="MIYPEIRNIHSPDLEPPNLPEDPGDCEISFEVFIGPKDGEGEEA
                     FQFTVVTPARLARGGEARWGRGKLILPSFEWAAVAQSVAKLLAQCGRPTWGEVASELN
                     KELLWEFDGRQPQVVN"
     gene            28292..29149
                     /locus_tag="VFA54_08415"
     CDS             28292..29149
                     /locus_tag="VFA54_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HZS50868.1"
                     /translation="MPAGRAPSPILPGKAEASGHRRRLIAFGWYGGKFNHLKWLLPLL
                     PKCHHYCEPFGGSAAVLLNREPSPVETYNDIDGEAVNFFRVLRNQTDELIRLVGLTPF
                     SREEFYVAVSPADRPLPEVERARRFFVRARQARTGLAQTASLGRWANCKNTSRAGMSG
                     AVSRWLGSVAGLSEIALRLLRVQIENRPAEEVIRVYDDPRTLFYCDPPYVHETRGDGS
                     AYRFEMDAGGHRRLAAALQRSRGKVAVSGYRCDLMDFLYRGWRRFDAPAKRCHSIKKM
                     RQECLWMNY"
     gene            complement(29451..29807)
                     /gene="ndhC"
                     /locus_tag="VFA54_08420"
     CDS             complement(29451..29807)
                     /gene="ndhC"
                     /locus_tag="VFA54_08420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZS50869.1"
                     /translation="MPREYIPILIFLAIAIAFPVVTILIARLVRPQFPFKEKLEAYEC
                     GIQAASDSRGRYTVRFYIVAILFVIFDVETIFLFPWAIQYSVLGLFGLVEMLVFLAIL
                     IVGYIWIFRKGALEWV"
     gene            complement(29834..31108)
                     /locus_tag="VFA54_08425"
     CDS             complement(29834..31108)
                     /locus_tag="VFA54_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZS50870.1"
                     /translation="MTWALGDRRCSGLALAVLAALCACHPREARRSPAIGEAYVAPET
                     LPLRREIALNSGIAATLKRGDRVEIVQRRRRFLKVRAPTGQEGWTEQGQLLTPEVHNQ
                     IGELARFAAGLPKLGVYRARDKLNLHLEPYRWSPAFFQLNEDQKVDLLARRVTERTAA
                     PPEPVAQPAPAAALSKKAGEPRGPKIDNEPPAQTAPGGSAARAAAQSANAIPPEPAPP
                     PPAGGAPSTSPQPEKPKQYDEWYLVRTLDAGDKGHAGWALARMLDADIPDEVAQYAEG
                     RRITSYFPLAEVTDGGQVKKIWLWTTIDRATEPNDFDSFRVFNWGRRRHRYETAYIER
                     RIKGYFPITVAPSVKTRYGSGPGFSLVVEKSDGRRYTRSYVMLGAIVRLYAEEPAQAP
                     ATVAPAVKPAAPAPPAQPTSFWKRLRAQLQRR"
     gene            complement(31260..32141)
                     /gene="truB"
                     /locus_tag="VFA54_08430"
     CDS             complement(31260..32141)
                     /gene="truB"
                     /locus_tag="VFA54_08430"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZS50871.1"
                     /translation="MDGILVVDKPAGITSHDVVNRVRRLARIQRVGHLGTLDPIATGV
                     LPLALGRATRLAQFFLHREKTYEAVIRFGFATDTYDSAGLPAGPDVPVALSAERLEEL
                     LAGYRGEFDQIPPPISAKKVNGVAAYKLARKLRPVELEPVRVRVDEFTLLGVEGSRAK
                     VRIRCSAGTYLRALAHDMGQKIGTGAHVENLRRLAAGEFTLDMAHTLEQIRDLAAQNR
                     LEEALIRGADLLPEAPAEQVDAITAAQILHGRDFRVSPFRVRPGSRLVKAIDMQGRLL
                     AIGEVKLPNLYHPIVVL"
     gene            complement(32206..32883)
                     /locus_tag="VFA54_08435"
     CDS             complement(32206..32883)
                     /locus_tag="VFA54_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50872.1"
                     /translation="MKLVVVGGHSRNIGKTSVAASVIAATRDLGWTALKLTQYGHHLC
                     SADGRVCDCILEDPAHPFSITREESRDGHHDTSRFLAAGAAEAYWVRTPQGGLARAMP
                     EIRRLIDGKGCVIMESNSVLRFLKPLLYLVVLDPSVSDFKASARDHLDRADVFLILDR
                     PPGTSAAFAPLPPGSGGASNIASEFRPAWDSVSSKLLERRPLYRVRAPDSLPEDLVAL
                     LRVRLQS"
     gene            complement(32880..33896)
                     /gene="lpxD"
                     /locus_tag="VFA54_08440"
     CDS             complement(32880..33896)
                     /gene="lpxD"
                     /locus_tag="VFA54_08440"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZS50873.1"
                     /translation="MTVGEIAGALGAPVEGDSSMQIARVQSLEDAGPADISFVDSRRG
                     RRMPPTRAGCLLVAPDFSNRERRTVIRVPAPRNAFARVIALLHPPRPVKPGVDPTACV
                     AATARLGDGVSVGPLAVIGENAVIGPRAIVGARSVIGDGVVIGEESLLHFGVTIYPGV
                     RIGQRAVLHSGCIIGADGFGFVLENNRYEKFPQVGTVEIGDDVEIGANTCIDRAALGV
                     TRIGDGTKLDNLVHIGHNCRIGRHVVIAAQTGLSGGVEIGDYAVIGGQVGIGDKARIE
                     SRAVLGSGCGVLTSKIVRAGEPVWGTPARPLKQYLERLALFDRLPKLISSLEKKTAQA
                     DTEK"
     gene            33984..34769
                     /gene="lpxA"
                     /locus_tag="VFA54_08445"
     CDS             33984..34769
                     /gene="lpxA"
                     /locus_tag="VFA54_08445"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HZS50874.1"
                     /translation="MSVIPGARIAPTARVSERAQIGEGVEIGDFCIIEEDVVIGPRTR
                     LEPYVMVKRWTSLGADNEISAGTVLGTDPLDKAFTGKRSYLKIGNGNKIREHYTISRG
                     TEPESVTEIGDDNYIMTSGHIAHNCKIGSRTVICSCALVAGYVEVEDEAFISGGVVIH
                     QYSKIGRLAMIGGNTRVNLDVPPYFLYTDFNVAPRGLNLVGLRRAGLKEQDVRLLKRA
                     YQLLYRSGLKLEDALEAIEREAPGEHTQHLVDFIRRSKRGICR"
     gene            complement(34807..36444)
                     /locus_tag="VFA54_08450"
     CDS             complement(34807..36444)
                     /locus_tag="VFA54_08450"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZS50875.1"
                     /translation="MATSQVESGDTLAPRASVVSLGAAAMAVPYAVAFVSSMCIMVVE
                     LVAGRLIARHVGSSLYTWTSVIGVVLAGIAIGNSAGGRLADRYRPASALGIVFSLASL
                     FCFAVPGLNSIAGNWGFLLRQEWATRIALHVFMVFFLPSAVLGCIGPMAAKMALDVGR
                     ETGRTVGNVYAWGALGSIVGTFLTGFYLIAKMGTIAVIVSAGLGLAMVAVLFGARTLV
                     PYFWGAAALCLIGASLGPWPWAQKFGTKIGFRRERYSNVLYTDESQYSFIQIEEESDP
                     PGTRSLSLDHLIHSYYVPDNPNDLQYDYEKVYASITEDAIGSRSGASALFIGGGGFVF
                     PRYLLSRWPQSYVEVAELDPRVTEAAFRAFGLPRHTPMHIFNLDARNHVDDLLRRKHA
                     GERVPSFDLIYGDAFNHYSVPYHLTTLEFNLKLRELMSPNGVYMINVIDIYASGQFLG
                     GILNTFAKSFPHVYAFSTVENGPSEEPDRRDTFVVVGSMQPLQGNPLKPGVGASELKP
                     EHFNALRQRSHNVVLTDDYAPVEQMLEPVVKRAEKEG"
     gene            36542..36838
                     /locus_tag="VFA54_08455"
     CDS             36542..36838
                     /locus_tag="VFA54_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50876.1"
                     /translation="MRRRESCAILAASFLALAVQRSARGQQSKEQKDEKPARIDIETL
                     RKQVDAKKDMFFIDVREPKEIEELGSLKGYVNIPVGQIESRLNEIPKDRYIVTA"
     gene            37124..37909
                     /locus_tag="VFA54_08460"
     CDS             37124..37909
                     /locus_tag="VFA54_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS50877.1"
                     /translation="MAFQHCNVRARLRERGFLAGSFVEIAAPEIVEILGSAGMDFAVI
                     DCEHAALRGPALANLIRASASTGIAPIVRVRENRPAEILEALDLGAAGLHIPQVATRE
                     QAEAAVRAARFPPAGERGFNPFVRAAGYGSVEVDQFRRQSDEETLLVLHVEGGLAVSC
                     AEAIVSVPGVDVAFLGPYDLSMALGIPGQVTDARVLRAARAVRGAAERYGVALGAFAN
                     SVEHARVWLNEGVRYLAYGVDSRMFLGVAAEVRRQIDAMRPQK"
     gene            38011..39687
                     /locus_tag="VFA54_08465"
     CDS             38011..39687
                     /locus_tag="VFA54_08465"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZS50878.1"
                     /translation="MVLIILAVLLAGAMGAQNAAAQEVTFQRLVAADKEPQNWLSYGR
                     TYNAWRYSPLDQINRSNIRKLVAVWSFQTGDVEGGLQCTPLVADGVMYISTSWNRVYA
                     LDAATGKKLWHYVYEKPKQVAAQFAPWNRGVALGYGLVFMGTLDNHLVALDAKTGREV
                     WNVTIEDAGQCGCNVTGAPLVVKDKVIVGVTGGDTAHRGYINAFDARTGRHVWRFWVI
                     PGPGEPGSETWKGDSWKFGGGSSWMTGSYDPELNLVYWGTGNPAADFYGDDRKGANLY
                     TDSTVALDADTGKLKWYFQQIPYDVWDFDAVYENVLLDLPYKGQKRKLLLNVNKGGYT
                     FVVDRTNGKFVSGWPVVENLNWIKGTDEEGNLVGRNEPVLGKPNFICPAVAGGRSWNH
                     GAYSPKTGWFYTTGLEWCESLTPMVQKPLEGKPFMAGDFEMKPPPKGEPGGHIGAYDP
                     ITGKQHWIYRSKYPLLASALATAGDLIFTGDPEGQFFALDAVTGKKLWSFNTGSGHRG
                     SPITYSVKGRQYIATPSGWGSSLAGLMPQLWPETEAFQPGSAIFVFALQE"
     gene            39684..40604
                     /locus_tag="VFA54_08470"
     CDS             39684..40604
                     /locus_tag="VFA54_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS50879.1"
                     /translation="MKRWASPRKIASGKPTSLRWKLACGALAGCLTLRAQAPNINLDD
                     PAVINQGRELFAATCSVGYCHGKEGRAGRGPRLAGRKFERDYLFRTITNGINKSLMPA
                     FKSQLSQEQIWEAVAYIQSLSGDASGSPTPKAGSASVPAPSAVLARPPQVAPADPNAG
                     DSEAGRALFFDVSNPRHCAACHQFQGKGGDVGPDLTSVAARPSKEILRDILNPDARLA
                     AEPVTVVTKSGERITGVKKQEVRDRLRIYDTSASPPVLRTIYKDQIQSVIPERVSPMP
                     HDYGQTLTRKQLLDVISFLKSASVSPREIE"
     gene            40754..41113
                     /locus_tag="VFA54_08475"
     CDS             40754..41113
                     /locus_tag="VFA54_08475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZS50880.1"
                     /translation="MPVRKILRAIRRAKPLTREEGMNILMRDRFQCQYCGLDGAASLE
                     NGLMMTVDFIVPRALRGKKDPQNLVAACRPCNLLKGRRRFASFEEAKKYVLARREEQR
                     REWEKNVALLRQQKATA"
     gene            complement(41283..41597)
                     /locus_tag="VFA54_08480"
     CDS             complement(41283..41597)
                     /locus_tag="VFA54_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50881.1"
                     /translation="MTESSGREASQGSASRGKVSFQIMATMEKPQAEAILGRSPDDLS
                     IEERRALAGSWIALEIYTPKTLPLRRIEAVGESVEECIQMLRRRNLDPTKFEFSRLKP
                     AY"
     gene            complement(41722..42069)
                     /locus_tag="VFA54_08485"
     CDS             complement(41722..42069)
                     /locus_tag="VFA54_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZS50882.1"
                     /translation="MRAPPWAVVLLFAAGAAAADHKLPPWREHSRMGQDLYRENCAVC
                     HDIDKDQKQTRKIGPSLNHLFKNEKLPLSGGKPTRDFVVVKIKFGGQLMPAFIKKMND
                     AQINALVAYLESR"
     gene            complement(42066..42572)
                     /locus_tag="VFA54_08490"
     CDS             complement(42066..42572)
                     /locus_tag="VFA54_08490"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HZS50883.1"
                     /translation="MYLMSEFSDQFTLAGAIQALKMRGADAADLDVFSTEPVDLPAGL
                     LDRPSRMSLLTVCGGAGAGLLSVLFVYWTQHNYEIVTGGMPYFSPWATGVIHFEFTML
                     GAVAGCFLTFLWESGILKREKRAPVPKLASGVLYLRVRCEPSQMLATGECLYQAGASK
                     VEKLEERS"
     gene            complement(42556..43869)
                     /gene="nrfD"
                     /locus_tag="VFA54_08495"
     CDS             complement(42556..43869)
                     /gene="nrfD"
                     /locus_tag="VFA54_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZS50884.1"
                     /translation="MATTLRTLPHDQIQEDTWRTLRNTSPQYVVAVLLTGSVTAWGLI
                     CWAYQISKGLGVVGKTRPTMWALYILNFVFWIGISHAGTLISAILRLTHAAWRKPVTR
                     AAEAITVFCLMIGGLFPIIHLGRPWLFYWLIPYPNSRLIWPNFRSPLLWDLTAILTYL
                     TGSMLFLYLPMIPDLGQLGLHTSGARGLLYRTLSLGWTGSDREWHALEKAMKLMAGMI
                     LAVAVSVHTVVAWDFAMALAPMWHSTIFGPYFVVGAIFSGIAALLLIMAVIRKTMGLQ
                     AYLQPRHFENLAKLLFLMSLLWAYFTVAENLTVWYGNQPHEMAVFGARVRGRYAPFFW
                     SMVICNFIVPCVLVGIRRLRSIKTAVISAVCVLAGMWLERFLIVIPTLSFPPLPSAWG
                     TGYNATWVEISIAISTFAAMALLYLIFAKLFPIIAIWEYKYVPDE"
     gene            complement(43877..44602)
                     /locus_tag="VFA54_08500"
     CDS             complement(43877..44602)
                     /locus_tag="VFA54_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZS50885.1"
                     /translation="MGVQIVPEQQAATTNHRWGMAIDLDRCTGCEACVVACHAENNLP
                     VWNPTEAARGRTVHWIRVDRYYEGEFPDVKVKYMPVLCQQCDEAPCEPVCPVYATYRN
                     PEGLNVQVYNRCVGTRYCGNNCPYSVRFFNFFPPQWDAPLELQHNPDVAVRMVGVMEK
                     CTFCIQRIQRAKLDAAADGRPLADGEVKPACVQSCPAEAMVFGDLNDPESKVSRLAAS
                     GRATRLLEDLGTKPKVFYLQRAK"
     gene            complement(44587..46779)
                     /locus_tag="VFA54_08505"
     CDS             complement(44587..46779)
                     /locus_tag="VFA54_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZS50886.1"
                     /translation="MDRRDFFKIFSTASAGLMTGACGKKLDTYIPLLVPDQEIVPGAE
                     QWYPALCGECPSGCGVIVRVMESERVIERNGEKFRQKIAAVKKIEGNPLDSVSGGRLC
                     ARGQAGVQSLYNPDRLRGPMHRKGPRGQADFIATSWDEAIAVAAEKLDRVRQENPSQI
                     VFLTGPQPGARALAIARFLESLGAPPPACFELVDFPLERKAAEQVYGWDGLPVYDLSN
                     ARYALGIGADFLGGWTSPVFYARQFGHFRQGRSAVRGRLVQAQSRFSITAQSADEWTP
                     LLPGSELSFALAIGHLLLSEKMARQPEELPDPVRQTFESVDPASAARACGLDERRVRR
                     LARELGESEAPLVISGASAPQTNSLAALVAAAYLNVMLGNVGRPGGVWPPAPGAVENR
                     AAFSNAIPLLERAQYLFLDGSNPVYTLPASAGIAEKLGRIPTSVSFSPFLDDSAAYVD
                     LLLPDHHPLEASNVVFPLIAPGPAVVVTTQFVQPLYDTRATEQVLAAIAAKMGLSFQP
                     APPRSVAERILPQGQSWDDVVEQGGFWGEPGSDSSPGSRPARSRASSPTANAAGPVID
                     STPAVFSGEAAQFPLYFQPYLSLQFQDGRGANLPWMQELPDPVSSAMWGLPVEIDTRT
                     AASLGIQNGDLVRVESPHGHIDAPAYVHPAAIPGVVSMAIGGGHKCYGRYAGRGSNPL
                     SILAPVWEKTTGALALGATRVRLSKLGPERQLIQFSANDREQGPWGYR"
     gene            complement(46786..47244)
                     /locus_tag="VFA54_08510"
     CDS             complement(46786..47244)
                     /locus_tag="VFA54_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZS50887.1"
                     /translation="MNYRWLISLCAAAALAEQPILFPHNRHMALGLACLDCHSGADER
                     AAAGIPSVNKCMLCHDKLATDKPGVKKLREYAARKVEIPWERVYGFSPEALVKFRHAP
                     HYRSKIDCTTCHGDVAKATVAVLAVKHNMGTCITCHRQHGASQDCATCHY"
     gene            47280..47948
                     /locus_tag="VFA54_08515"
     CDS             47280..47948
                     /locus_tag="VFA54_08515"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZS50888.1"
                     /translation="MTANGKKRLFAIILTAIAAGVGYTLWRSGIFHQITNKDELVQSL
                     RREGLAGPLLCIGLQFAQVVIFVIPGEITQFAAGYVFGAGLGFVYSILGIMLGSAFNF
                     YFARVFGRPVVEKFIRPETLNKIDNALNNAKGKSALFLLFLLPGLPKDAMSYGAGLSR
                     ITLAEFVFISGLGRTPALVLSVLLGSQAYNQDYRRMVITAIAAGAAIGVYILYARRRR
                     QQPT"
     gene            48024..>48760
                     /gene="folP"
                     /locus_tag="VFA54_08520"
     CDS             48024..>48760
                     /gene="folP"
                     /locus_tag="VFA54_08520"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZS50889.1"
                     /translation="MPRKRYEWRLQNRTVILGVRTLLMGVLNVTPDSFSDGGRYLDPD
                     AAYARALEMEEQGADLIDIGGESTRPGSRRIPAREELGRIAPVLKKLRNKLNIPISVD
                     TYKAEVAERALELGAEIINDVSGLTFDPALAEVINRRDAGLVLMHIRGTPETWSQLGP
                     LPDVMGTIARDLDAALCRARRTGIDRRRIVIDPGIGFGKRGDQNYEIIVSLGRLAVLD
                     QPILVGTSRKSFLGHPARDREDLRLFGT"
BASE COUNT         9642 a        14691 c        14882 g         9545 t
ORIGIN      
        1 cgaaaaacgg gtggcttctt tccaaccctt gatcacaatt ttgcctgctc ttgagtcgag
       61 cgcaatcatc acgcgttcgg cgggcaaagc ttccagaaat ggaacattga cgttttcggc
      121 agtgaaagcc gccgtgccga cgatcacgcg atatacgccg ttgtcgagca ggtccatcgc
      181 gcgatcgacg gtgcgaatgc cgccaccggc gcggatgatg gcgcgggacg ccatctcgcg
      241 cacgatgtca tcattcgagc cccgatccat ggcagcgtca agatcaatca cctggatctc
      301 agggaaggct tccaatctgg cgagcatttg cagagggggc tccgcttcga gcgccttctc
      361 gcggccctgc cgtagctgga ccactttgcc tcccatcagg tcgatgcagg gaacgatcat
      421 cgcggaatcc tgacgggaat atggcgtcgc gcgaggactt ccttgaggcc ggcgatggaa
      481 tacgtgccgt agtgaaaaat tgaggctgcc agggccgcgt cagcctctcc ttcctcaaaa
      541 acgcgcacaa agtcctcggg acgtccgccc ccgccgctgg cgatcacggg aacgctggtg
      601 gcgcgagaaa cagcccgcgt caattcgcaa tcgaagccgg cctgagtgcc gtcgcaatcc
      661 atcgatgtga gcagaatttc gcctgctccc atttcctcgc cgcgccgggc ccaatcgacc
      721 gcatccaggc cggtatcgcg ccggccgccg taagtgaata cgtcccagcg ttgacgcccc
      781 gaggaatcct gccgccggcg ggcgtcgata gccaggacaa ctgcctgggc gccgaactct
      841 tgcgaaagct cagtgatcag ttgcggccgc tcaaccgctg ccgtattcac cgagactttg
      901 tccgcccctg ccagcaaaat gcgccgcgca tcggccacgc tccgtacgcc gccgccaacg
      961 gccagcggga tgaaaacttt gcgggcggtg cgctctacca catcgatcag aatatcgcgg
     1021 cgctcgctgg atgcggtgat atccaggaac actagttcgt ccgctccggc ctgattgtac
     1081 gcgctggcga gctcgaccgg gtcgccggcg tcgcgcaact ggaggaagtg gatgcccttc
     1141 acaacgcggc catccttcac atcgaggcac ggaatgatgc gtttggccag caagactaaa
     1201 atcttcctcc caaaaaattg gccaggatcc gctggccggc cgcgccggat ttctcgggat
     1261 gaaactgcac gccgaagacg ttgccggcct cgacagcagc cgcataagta atgtggtatt
     1321 tcaccagggc agcggcccgg cttccggcgg cttgttcggg cacgtaataa ctgtgcgcga
     1381 agtatacata aggatgctcg cccaatccgc gcagcaatac cggggagccg cacagttcga
     1441 gcttgttcca gcccatgtga ggagcgcgga ttcccggagc gaatctttcg acgctgccgg
     1501 aaaacaggcc gaggcccggc acatcaggcg cttcggcgct gcgctcgaat aaggcctgca
     1561 tgccgaggca aattcccaga aaaggaacac cggcccggag gcgttcgatg agagcgccgc
     1621 ggacgccgag ccggtcgagc gcccgcatca tctgaccgaa atggccgacg ccaggaagaa
     1681 taattcgatc ggcgcggcgc aggccatccg catcgcgagc aaattcatag ccggcgccca
     1741 gcgcgcccaa catgttttcc accgagcgaa gattgccggc gccgtagtcg aaaatgacgg
     1801 tcatggtgat tcttcggtat tgctgatcat ttggatccgc cggtctgaat cacccggccg
     1861 cagcgcaccg ctggagcgca ggctgcgcgg cgtcccaaga tccggccgga gaacgaaaat
     1921 ctcacagcag cccctttgtg ctgggcaact ggttgcgcag ccgccggtcc ttggaacagg
     1981 catatttcat cgcccggcca aagcacttga agatcgcctc gattttgtga tggctggacc
     2041 ggccatagag cacgcgggcg tgcagattag cgccggcgtg attcacgaaa ccgccgaaaa
     2101 aatcctccac gagttcggcc tggagatcgc ctaccttgcg gacccggacg cgatctttat
     2161 acacaagcgc cggcctgccg ccgagatcca ccgccaccac cgccaacgtc tcgtccatcg
     2221 gaagaaggaa ataaccggcc cggttaatcc ctttccggtc gccgagcgcc ttggcaaagc
     2281 actgccccag cacgatgccc acgtcctcca cggtgtggtg ctgatctaca tcgaggtccc
     2341 cccgggcagc gagttccagg tcaaagccgc cgtgtttggt gaacagctcg agcatgtgat
     2401 cgaaaaagcg gatgccggtc gagatgttgt aacggccccg gccatcgatt ttcagccgcg
     2461 ctgttatctg cgtctccttg gtattgcgtt tgacggtagc ggctctcata accacgcgga
     2521 ctgcaatccg ttttcaagtt tcactaaata ttttgaggtc gccagcaagg cagccccgca
     2581 gtgcaagcgc ggaggggcgg cggctggcgg gttccggctc acagtacgtg ctccagttca
     2641 ttgatgtcgc tgataatggc gcgtgcgcct tcctgccgga ggagttcgat tgtttcgttg
     2701 cggcggccgt tgccggcggc ggcgacgccg ataaaaggaa tgcccgccgc ccgggcgctg
     2761 cgagcatcat cgacgttatc gcccacgtac acggcgtcga ttccagggtg tgcgctccgg
     2821 attttcaaga tgccatccgg agcgggcttc agattggcga catcggtgct gcccacaatc
     2881 ggctcaaata acaaatgcgg ggcgcagcgg tcgatcgtca agcgggcctc acggcgcgtg
     2941 cggcccgtaa aaatcgcaaa gcgccaatct ttctgcaagc gctcgagcag accgtcccgg
     3001 gccatccatt tttcgcgcaa aatgagcgcc tcgccccggc cgtctccgtt ctctcccagg
     3061 aaaaggctct gaaaatagga aaccacttct tcatacggcg tggggaagcc aaactcgtgg
     3121 atcacggttg tagacaacgc ccagtcatcg ttgaagccgc cccgattctt aaactgctgg
     3181 ataaaatcgc gggtgacgcg gcggccactg aaatgctgga ccgtgcggat gatcgtttca
     3241 tgatacgaat cgctgacatc caccagaact ccgtccagat caaaaatcag cagcctgctc
     3301 atccgagaac ctcgcgcaaa acgtgcagga agcggcgaag ctgagctttt cgcccgacgg
     3361 tgatccggat gcagcccgga acttcgtagc tccgatcgcg cacgagcact ccctttgccc
     3421 gcagccggtc gcgaatttca agggaacggc tgccaaagcg ggccagcacg aaattcgcag
     3481 cgcttggata gtagaaaatg cccaatttgt cgagctcctg gcagagcgcg cggcgggatt
     3541 ccagcgcttc ggcgacgtag tcccgcacat aaatttgatc ctcgacggcc tcacaggcgc
     3601 acaccacggc cagagcgttg acgctgtaag gcgattgggc cttgtgcagc cagccgatgt
     3661 tttcggcctg ggaaaacaaa catcccaacc ggagagcggc catgccgtat actttcgaga
     3721 acgtgcggct gacaaagagg ttgggaaact cgccgatcag gccgagagcg gtcgttccgt
     3781 aaaattcaaa gtaagcttcg tcgatcaaaa ccgccgcgcc tgctgctttg cctagaatgc
     3841 gccggattcc gtcgagattc accggcgttc cggtcgggtt gttcggattt gcgatcagta
     3901 tggcgcgcgt ctccggcgta atggagtcga gcaattcctc cagcgggaag gagaggtcgg
     3961 cgggccggta cggaatttcg aggatccgcg ctccggctac ttccgagtag aagcggtaca
     4021 tggcgtagga gggcgccagc agcagaacgg acgcaccgcc gtccacatag gtattcacca
     4081 gcacctgaat cgcctcatcc gtgccgttgg tgaagagcaa ctcgccggga cgcatgccga
     4141 agaaagccgc cagcttctcg cgggcttcgc cgtactcagg atagacggcc agccgctccc
     4201 gcgtggcaag gcgctttagg gcctgcaaga cacggggcga gcatcctgag gtgttttcat
     4261 tgaaatccag gcgtagcttt ccctgccggc cggcagtcgg cggcgagtaa ggggccattc
     4321 gctccacagc cgcccggggc tgaggccggc gggcggctgg agcctgggtg gcagcagcca
     4381 gatccgtctg cattacgaat cctttctttt atgcccactc ggggatcccc ggcggggttt
     4441 gcccgtcgcc tcgagcctcg cctcaacaga tcgcgcatgc ccctcgagtc cctcggctcg
     4501 cgccagagtg gtgatcgctt ccgccatata ctgcaagccc tgccggccca gttcctgcac
     4561 ggagatcacc ttcacatagt cgtttacgga aaggccgccg cgcagagccg cggctccccc
     4621 ggtaggcaac acatgattgg gtccggaagc ataatcgccg gcggcctctg ggctccaggg
     4681 gccgacaaac acgctgcctg cgttcgtaat tcggctgaga aaagacgcct cgggaatgga
     4741 aatatgctcg ggggcgaacc ggttggcgat atcggcggct tcgtcaagtg agcgggtcac
     4801 gatgatggct ccattcgacg cgatggattt tcgggcgacg gcttgggtat tcagcctgct
     4861 caactgctcc tcgacgccgc gggccacccg catcgccagc cgcatcgagt ccgtcaggaa
     4921 aatggcggtg gcgtcggcgt cgtgctccgc ttgagccacc atgtctgctg ccacccaatg
     4981 cggccgcgct ggcggttttt tatcgctggc aatgatgaca atttcggtgg gaccggcgac
     5041 aaaatcgatt cccgtttcgc cagccagcag ctttttggcg gcagccacgt agatgtttcc
     5101 cggcccaacg atgcgattag ctcgaggaac ggtttgggtg ccgaacgcaa aggcggcgat
     5161 ggcttgcgcc ccgccaattt gaaaaaagtt tcggatgccg agcatgcccg ccgcgcccag
     5221 cagcagcgga tggcggctag gcgatgcaac cgaaatcgtc ttcacgcccg cgacttgagc
     5281 agggatgacg gtcatcagca gcgtcgaggg caggggatag cggccgccgg ggatgtaagc
     5341 ggccaccgaa tccaggggtc ggacgatctg gcccaccgac agccccgctc gcacgcgctt
     5401 gtgccaggtg cggggcatct gcatctggca atattcccaa atctgtcccg aagcgagttc
     5461 gaccgcccgg cggaagggcg ccggcagttc gagggcagct tcgtccagga tcgctggatc
     5521 caggcggatc tctttgccgc aaaagccgtc aagctgccgg gcatactcga gcagcgctcg
     5581 atcgccgcgc cggcgcacgg cttcgatgat cggctttact acggcttccg cctggagaag
     5641 cctgcggctg cgcctcaaaa tcaggctttc aacctgtttt cgatccctgc tgcggataat
     5701 tctaagcatt gtgtgcaatt agattcgcaa agccttacat cacgatcttg ttcagcggat
     5761 attccacaat tccttcggcg cccgctgcct tcagcctggg gataatctcg cgtactgtgg
     5821 attcatcgag caccgtgttg accgccgccc agccctcctc gctcaacgag gagatggtcg
     5881 gccgcttcag ggccggcagg acggcgagca ccgcaggaag acggtcgcgg ctaacgttca
     5941 tcattagtcc gacctttccc agcgcattga tcgctccttc cagcagcatc ttcagatcgc
     6001 caagcttctt ttgcttccaa gggtcctgcc acgccgcgcg gttagcgatc aactgcgtgt
     6061 tcgactccgt caccgtgtcg atgattttca gcttgttcgc gcgcagcgac gagccggttt
     6121 cggtcacctc gacgattgcg tcggccagca cgggcggctt cacctcggtg gcgccccaac
     6181 tgaattcgac tttcgcgcgc acgccgtttg cggcgaggta gcgctccgtg gtgcgaacca
     6241 gctcagtggc gatgattttg ccttccagat cacgcaccga gcggatggct gaagactcgg
     6301 gcacggccag cacccaccgc accttgccga agctttgctt ggcgtaaatc aaatcggcca
     6361 ccgccatcac atcggcgttg ttctcctcga tccagtcctt tcccgtcaat cccgcgtcga
     6421 gaatgccgtc ttcgacataa cgggccattt cttgcgcccg aatcagcatg cattccatct
     6481 ccggatcatc gattttcgga aaatacgaac gggcgctcgg gctgatgtta tacccggcgc
     6541 ggcgaaacaa atcgatcgtc gcattctcca ggctgccctt gggaatgccg agcttcagac
     6601 tcattggctg cctccgtaga ccgccgcggg atcgaatgtg cgctgctcag cggcccgcca
     6661 ttctccaccc tcgaggcgcc ggtagaaaca gcttcggtag ccattgtggc aaacgccggg
     6721 gcctagcgcg tcggcgcgaa tgagtacggc atcctggtcg cagtcggtgg aaatctcgcg
     6781 gacctcgagc cagtggccgg aagtttcgcc cttggtccag agtttcttgc gagaccggct
     6841 gaagaacgtc accctgcctg tagcaacggt ccgctggaag gcctcggcat tcatgtaacc
     6901 gaccatcagc acgcgccctg tggaggcgtc ctggacgacc gcggtcacca ggccatcgag
     6961 cttcgtgaag tcaagttcca tatgaaagaa acacccgcca acaaaaaagc ccgctcggga
     7021 gttttctctc cggcgggcct gcttgcaaac gcttgagtga aaattaccgc ttccgccgga
     7081 gcccaccagt agatcgatga tggtgatggc tgcggcttgc ggcgtatggg cgcattgcga
     7141 aaaaaccagt atagccagcg caggcgaccg gcgtcaacca aaaatcgcag gcccgccgcg
     7201 ttctaggcgg cttggtgtgc agcggcatga atgtccgccg ctcgggcgaa acagccggca
     7261 tggcgcccgc cgggctgccg attaggtatt gctagttttg tggtttcggc cggggtgtgg
     7321 cgtcgccttc cgtgaggccc aagacccagc gaatcgcttc cagccacatc ttttgcatat
     7381 cgggacggtc ccaggcttcg ttttcatgac ccagcgagca gtaaaagacg cgccctttgc
     7441 cgaagcgtcg cgcccaggcc accgggaaat cgccgtcggt gcgatgcaca cccggcttgt
     7501 gaaggtcaat cgaggcgtta tcgatgctta gcagcacgcg cacgcgatcg cgcgaaaaat
     7561 ttctgaactg ataaatctca tcattgatgc tgaaagtaga aggaaaatgg cgggtcgccg
     7621 ggaatgcgcg gtcctcgacc ttaatccgca cttgctggtg ccagggatga ttatcaaagt
     7681 atccgccgat cagctcgccg tattcaggcc actggtagaa cgtgtctgtc gcgctatgcg
     7741 ccccgataaa cccttttccg tcgtcccgga gaaacgagag gaaatccgcc ttctgcgagg
     7801 catccatggg cagttcgccc gtggtgtaga aaaaaatagc gtcaaagtaa ttcaggttct
     7861 tggcgttgcg ctcgaggggc ttcttcgtga tcaattggac atcggtccgc agggtcacgt
     7921 caaaaagacc cgactcctct cccagctttc ccagcataat catgccgtga gaaatcgaat
     7981 cgtgggtgaa cccggcgctc tctccgatca ccagcaagtg tttcttttcg cgcggggcct
     8041 tggatgtctg ggccaggcag gccagaccca aaagaactac agccccggcc gcaacagcgg
     8101 tgcggattcg atgccccata tacaacctcc aggacgccag tatcatcgca ttccggcgga
     8161 aagactggca accgccccgc cgaccagcgc ttgatcaccg ggccccgcaa gcttgacacg
     8221 gcgggcaggc tcgcatacct tgcattcttg tggtccagcc acgccggttg cttgttcttt
     8281 ctctctctct tctcgcaatc ggctgctcgc gcggcagccg tcccgcgggg cctcgtctgg
     8341 tggcggcgat aaacgccggt gtggaaggag acgcgctgaa gaccgccgcc gcagattacc
     8401 aaaaggcttc cggggtccgc atggaggtcg tcgaactgcc gtactccagc ctgttcgaaa
     8461 aggaaatgct tgacttgacg agccgcacgg gcgcttacga cgtcatcatg atggacgatc
     8521 cctggttccc acggctggct tcggatgaca agctggctgc gctcgagccg ctttataaaa
     8581 aggccggctc cgcgggcccg gacgccgatt tcattgcttc ctcgctggcc ctgtgtcacg
     8641 agccataccc ggatggacgc ctctacgccc tgccgtacgt cgggaacgcc aactttttct
     8701 tttaccggaa agatttattc gaccgctatg gcctaaccga gccccgaaca tgggacgatg
     8761 tcttgctggc agcgcgagaa atacggagcc gtgagaagaa tgtctacggc tatgtgatgc
     8821 gggccgctcc cggcaatgcg gtgattgcgg actttatgcc tgtgctgtgg gccttcggcg
     8881 gcgagctgtt ttcggcgaac ggcgagccgg aactgaacac gcccgaggcc gccgcggcgc
     8941 tccgcttcat gattgagctc ggaaaattca gcccgcccgg atatgtgagc tttaacgctg
     9001 atgaggtcaa cgcctacttg ttgcaaggcc gggctggcat gtgcatcaac tggcctgcct
     9061 ggctgccggc gatggatgat cccgaccgct cgcgggtaat ccacaaaatc gcctatgcgc
     9121 cgatgcccgg cgagcgcgtg aagggccgtt cggcgctggg caactggctg ttggcgattc
     9181 cagccggatc caaaaacccc gatgcggcgt tcgcattcat tcactgggcg acccagccgg
     9241 cgcagatgcg tcgcgccgcc gagcagggga atccgccgac tcgccgcagt gtgtttcggg
     9301 acgccggcct gatgcggcgg ttccgcgctt tccccgtgca gttgcaggct ctcgaagagg
     9361 ggcggccaag acctcgcgat cctgattgga acgagatcga gaacgttttt ggcatttacg
     9421 tttcccaggc gaattcggga agtatttccg tttccgaggc ccttgcccgc tcacaggccg
     9481 agatcgcggc catccaccat aggagccgga aatgacggga gcatcggaag cgaaagcaaa
     9541 gcgccgctgg cgggagaaat acagcggccg cctgtggctt gcgccggctc tgatcgtgct
     9601 gggcgcgctt tccctgtacc ctttgctgtt tgcccttcag gtcagcttcc ggactccgtc
     9661 cggctatggt tttggcaatt tcagccgtct ggtcaccgac cgcctttttc tgatctcgct
     9721 tttccaaacg ctggtctata cggcgggatc gctggccgcg gagtttagca tcggcctggg
     9781 gcttgcgctg ctggcgaacc gtgaattccg gggccgcgga ttgctgcgat cggttttgtt
     9841 gattccgatg ctgcttcccc ctgtcgtcgt agcggtgatc tggcgcctga ttttaaatcc
     9901 cgaatttggg gtcttgaatg gaactctccg gtcgctggga gtcaacaccg cgaagctcac
     9961 ctgggcggcg gcggaaaata cggcgttgcc ctctgtaatg ctggtggacg tgtgggagtg
    10021 gacgcccttc ctgtttttgc ttctgctggc cgggctgcaa tctctcccgg ttgaaccgtt
    10081 cgaggccgct agcgtggacg gcgcatcaaa atggcaaatc ttccgccgtc tgaccctgcc
    10141 catgttgaag ccggtgattg caatcgctgt gctgctgcgc ggcatggatc tgctacgggt
    10201 cttcgatcag gtgttcatcc ttacgcaagg cggaccaggt ttcgcgaccg agactgtgac
    10261 tctctacatc taccggacgg cattccggtt ttacaacttc ggctacgccg tggcgatgtc
    10321 atttgtctta ctgatagcga ccacggtcat cgccaggtcg tttctccgcg tgctcggcca
    10381 gcagtcggca tcggcatgaa agcgctttcg aaaatcggcg gggcctgcct gctgggggcg
    10441 gctggggtta cgctttttcc tttttactgg ctcgcgacgc tttccctcaa gcgggaagtg
    10501 gatcaattcg caatcccgcc gatctggatt cgcttccacc cgactctggc acactatcac
    10561 gacatattct gggtgcggcc gttcggcttg tattttctga acagcctaat cgccgcctcg
    10621 gtttcgacgc tgctggcgct ggggattgga acccttgcgg cgtattcgct ggcccgcttc
    10681 cccaggccgg aagtttcctt ctggattctc tccacccgca tgttcccgcc catcgtcagc
    10741 atcattccgc tgttcctgat gctccggtgg gcgaacctgc tggattccgt gctcgctttg
    10801 gcagtgcttt atacggcttt caacctgccc tttgtggttt ggatgatgcg cggctttttt
    10861 gaggagctgc cgcgggattt ggaagaagcg gcgatgatgg acggagaaac gcggctcggc
    10921 gcgctgtgga aggttgtgct gccgctggtg cgcccgggcc tggcggcgac ggcggccttt
    10981 tgcctcgtga tcgcatggaa cgaatttctt tttgctttgg tggtcgccca gaccgacgcc
    11041 gcgatgactt tgccggtggg aatcgccagc cgcgtgacac agtacgagat caagtggggc
    11101 gccatgagcg ctgccggcgt ggtggcaatc ctgccggtgt tcctcttcgc cgcggcgatg
    11161 caaaaatatc tggttcgggg gctttccctg ggggccgtga agggatgacc gcttcagggc
    11221 ctgacgccgt gaggaaaggc gccgcgaaaa cggtttttcg ttgagagccg ccgggccggt
    11281 cgccggggcg gcgtggcttc ggctgcggat ccagcaggcg gcatccagcc gcggctcgaa
    11341 catccggcga acacgagctt catgcgctta cagattgaga aactcggcaa gaggttcggg
    11401 gccactcatg ctttgcggga tttctggctg gatatcggcg acggcgagct cctggttctg
    11461 ctggggccgt ctggctgcgg caaaacgact tgcctgcgta ctgtggccgg gctcgagcag
    11521 cccgatgaag ggcgaattct gctcggcggg cgcgatgtga cgtatctcga gccgcgcgag
    11581 cgcgacatgg caatggtctt tcagagctat gctctttatc cgcacctgaa cgtgtatgag
    11641 aacatcgctt tcccgctgcg ggtgcggaaa cgggccgaga gcgaaattga aaaacaagtc
    11701 aagcgagcgg ccgaaaggct cggcctggct gggctgctga aacgaaggcc gcgcgagctc
    11761 tccggcgggc agcggcagcg cgtggcgctg gcacgggcga ttgtacgccg gccgaacgcg
    11821 ttccttttcg acgagcctct ctcgaatctc gacgccaaac tgcgcgtgga gatgcgggcc
    11881 gaactgaagc ggctgcaaca cgaattggga gtcacctcta tctacgtgac gcacgatcag
    11941 gccgaggcca tgactctggg ccgccgcctg gccgtgttga aggacggcgt tctggtgcag
    12001 gccggcgaac ccgaagaggt ttatttacgg cctgctaact tgttcgtcgc cggctttctc
    12061 ggcagccctt cgatgaactt tctgccgggc cgtgtggatg cggccgggcg gcgatttgtt
    12121 gcgagcggaa tgtccatcgc gctcagccct gaacagctcg atcgcctgcc cgatggcgcg
    12181 ccggttacac taggagttcg gcccgaaaat gtagaagtaa gccggcagcc ccggcaggga
    12241 tggctggcgg gccgtgttta cgtaagtgaa tcgatgggaa acgaaaacct cgttgtggcc
    12301 gaagcgggcg ggcgccgcat cagttgccgc acaatcccgg aaatgcgttt ggattttgaa
    12361 gcctctatct ggctgttctt tcgaagcgac cggttacatt tcttcgacac agcatcggga
    12421 aaatctctgt ggccatgaat gctcctctgt tgtcgggccg gggcatccgc aagtcgtttg
    12481 ggacgaaccc cgtgctccgc ggcgtgaact tcgatgtgcg taccggtgaa gtgcatgtac
    12541 tggtcggagc caacggggcc gggaagtcca cgctggtaaa gatcctttat ggagccttga
    12601 cccccgacga gggcgcgctg tattggaaag acgcccctat agcggccggc gacttgcggc
    12661 agaccgccgc tcgcggcgtc gcttatatcc cccaggaaat cgaggtgatc ccgcacctat
    12721 cgctggtgga gaacctgttt ctcggcagcc cgatcctgcg gccgtggaaa tcgccgctcg
    12781 atgtcgtgga tcacaaggcc cagaccgcgg cggctgccga ggtcttgcgg cgcgtcggcg
    12841 tcgtccggga cccgaatacg ccttcccgag acctttcggt cgccgaacaa cagttgctga
    12901 tgatcggccg ggcgctcgtc cgcaaggtag aaatggtcat tctcgatgag cctacggctt
    12961 ctttgactcc cgtcgaggtc gataagctct tcacggtgat cggccagctt cgcagcgcgg
    13021 gcgtgggtct gatttacatc agccaccgcc tggaagagat tccccgcatc ggcgatcgcg
    13081 tttccgtctt gcggggcggc gagatggtat tcaccgggct ggcttccacg ccgaagcagc
    13141 aagtgatccg gcaaatgatc ggacatgacg tcgaaggcgc ccaggcccga gccgccgtcg
    13201 cggctgcccc ggcgatcgag ctgcgcaatg gaaccttggg aaagcgcctg caagacgtct
    13261 cgttttctgc gcgaagcggc gaagtgctgg ccttcgcggg attgatcgga tcgggccgca
    13321 cttcgctggc gcggtcgctt tttggcgccg agcccggttt ttcgggtgat ttgcgattat
    13381 tcggcgcgcc ggttcgcttc cagtcacccg ccgaggcgat ccgccacggc gtcgcgctgc
    13441 tcacggagaa tccgaaagag caaggcttgg tgctgactgc ggatgtggca accaatattt
    13501 ctctacccag cgcgccctta ttctcgcaat ggtgggggta cttcgaccac cgcaaagaag
    13561 cctccgtcac ggctgaatac gcgcggcgcc tcggccttca gccccccgac gtacatcgct
    13621 tggtgcggct gctgagcggc ggcaaccagc aaaaggtcgt catcgcgcgc tggctcctta
    13681 acggagcgcg cgttctcttg ttcgatgagc cgacgcgcgg cattgatatt ggcgccaagg
    13741 agcaggtcta cgattggatc cggcggctga cggcggaagg gaagacggtg ttgctttttt
    13801 cgtcggagac ggaggaagtg cttcgcctgg cggaccgcat tctggtcatg cgctcgggcc
    13861 gcattacggc cgaactttcc gcagccgagg caacagagga aaagatcctt cgagcatcgt
    13921 ttgactgaat cggggactca actgactgaa ccgggcgggg agccagggtg tgaatcggga
    13981 tactgcgtac ttgaccaaaa tcaggaatct ctcccgcgct cccgatggcc acagcaggcg
    14041 aggggatccc tgattggcct tgggcgcagg gaagatgtag cttgtcaaac cgggatcagc
    14101 ttgccgtgaa aaacaaagaa ggcatccagg cggcctgggt gcgtctcaac gcgatgaaat
    14161 tgctgcgccg tttcgggccg ctgctcggac tgctcattct ttcggcggcc ctcagcgttg
    14221 tttctcccta ttttctcact tgggacaaca tcctcaacat tttccggcaa agcgccgtaa
    14281 acggcttgct ctcgctgggc cagcttgtgg tcatattgac ggccggaatt gatttgtcgg
    14341 tcggctcgat actcggcgtg tgcacagtgg ttttcgccat gaccgtgaag gcaggggcgc
    14401 ctgcggtaat tggcttcgcg gcgtcgctgg cttgcggcct ggcgctgggc cttatcaacg
    14461 gattcctgct caccggcttg aaactgccgc atccattcgt ttcgacgctg gggatgatga
    14521 acgtggcgcg cggcgcggcc atgttgctct cgggcggctt tccgattccg gatttgcccg
    14581 ctggggcgcg ctatccagga gcggcatctg tggccggaat tcccatccag gtgatcatca
    14641 tgctggcaac ctttggattc tttggtttcc tgctgcgtca cacggttttc gggcgtgaca
    14701 tttatgccat cggtgggaat cccgaggcgt cacggctggc cggcatcgcg gtcaacgcgc
    14761 ggctcaatgc cgtctatggg atttccgggc tgctggcagg tctggcggct ttgctgctgg
    14821 cgggccgcat gaacgcgggc ttccccctgg cgggaaccgg cgccgagctc gattcgattg
    14881 ctgcagtgat catcggcgga gccagctttt tcggcggcgt ggggacggtc tccggagttc
    14941 tgatcggcgc cttgatcatc gccgtgctgc gaaacggttt gaacctgctg aatgtctcca
    15001 cacactggca gatggttgtc attggtatag tgataatctg ggctgtttgg gtggacgttc
    15061 tacgccgtcg cggagagaat cgttgatcag gaaactgacc ggtttggtgc tggttctggc
    15121 gctcgttttg ccgggctgcg ccagaggaac ctcctccggc cgcccgcaca tcgctgtagt
    15181 gctgaaggcg ctggacagcg agttctggct ggctgtaaaa cggggcgccg aatcagcggc
    15241 ggccggcaag gccgacctgg gcattgtagc tcctgagcgc gagatcaaca tcgaccagca
    15301 ggtttcgatg attgaagatc aggtttcgcg tggagcggcc gcccttgtgg tggcgcccgc
    15361 cgctgcggcg caagtagcgc ccgcgctgga caaagcagcg gcgcggggca tccccgtcat
    15421 catcatggac acagatattc actggcccaa gatggtcacg tatgtcggta cggacaacgt
    15481 gaagggcggc gaacttgccg gcgagtatct ggcgaaggca atgcccaacg gcggcgaggt
    15541 cgcgctgatc actggcgtgc cgagcgacga aacccatgag ctgcgcaagc ggggattttt
    15601 ggagggaatc aaaaagtccc ccaatctcag actggtggcg gaacagccgg cgaatagcga
    15661 gcgcgccctt ggccttacgg tgatggaaaa tatcctgacc tcccagccga acctcggcgg
    15721 agtgtttatt accaacgacc agatggccct gggagcgctt gaagccgtcg agtcgcggca
    15781 tacaaacaag ccggtgaagc tggtgagctt cgacgcgggc agcgaagttc ttaagatgat
    15841 cagcgagggc aaagtggatg ctgtggtcgc tcagcatcca tttgaaatgg gcaagcggag
    15901 cgtggaagcg gcgctggcgg cgatcgccaa acaaaaggtc gagccccgga tcgatactgg
    15961 gacagagttg gtcacgcgcg acaacgtggc ggcatttcgg gccgaatgaa tgcgacactc
    16021 gaagatgccg gaccgcgagg aacaaccggg tataatccgc cccctaatag cgcggggcgc
    16081 tgtatcggat agatgtcatg atgccgttgc tttataagcc gacaagcggc accggcgagg
    16141 tgctttgcgt atcgcaggag agagcaggat gggagaccgt ctcgctgcgt ctgattcgtc
    16201 tggagcggga ccagcgatat tcgatctctc ttcccggcga ggagctggct ctggtgatgc
    16261 tgggcggccg cgcctcgatt acggcgggcg gctatgagtg gtcgagtctt ggcggccggg
    16321 aaagcgtgtt ttccggtatg ccgcacacgc tctatcttcc cgtcggtacg ggacgggtgg
    16381 atatccgagg tctcaccgag tactgcgaaa tcgccgtttg cggagctcgg gctgaccggc
    16441 tttttccggc tgccgtgatc gagccttccg cggtggagat tgagatccgg ggcggcgaga
    16501 acgccacgcg ccagatcaac cacatcatga agccggattt tccggctgac cggatcctgg
    16561 tagtcgaagt ctataccccc agcggcaact ggtcttcgta ccccccgcac aaacacgatg
    16621 tgcacaaccc gcccgccgag gtagatctcg atgagatcta tttttataaa atctcccggc
    16681 ccgaaggcta cgcaattcaa cgcgtctata cccgggacgg atggcgggat gagaccatga
    16741 ccgtccggga caacgaggtg gttctgattc ccgagggcta tcacccggtc gtggcggctc
    16801 acgggtatga ctgctactat ctgaacgtgc tcgccggctc cgcccggtct atggcagcga
    16861 gcgacgatcc cgattacgcc tgggttcgca gctcctggcg gaacaaggat ccgaggttgc
    16921 ccctggtgca ttagcgcgcc cgcaggccgc cctgcacgtg gacagccggt gggcggccct
    16981 acagccgccg cctgtgaggg gccgccaagg cggagggctt ctctcgaaga atcgtttcgc
    17041 gccgcgcttg gatgattggg ttttttaaaa cgcaggagat tttgtgattc gcgttgccaa
    17101 cgctccagtg agctggggca ttatggaggt agaagggtgg agcccgccgc tgccgcctca
    17161 gatatttttg gacgagctga aagcagccgg ctacgacggc actgagctgg gcccctacgg
    17221 atatctccca accgagccgc aggctctcgg cgccgagctg aagcgccgct ctttgacgct
    17281 gacgtcggca ttcgtgccgc tccgcctgaa ggagccggga acggatttga gcgaagccga
    17341 acaggtggca aagctgctcc aggctttggg ggcgaagtat ctggttcttt cggacgctct
    17401 gtggcgggag cgcgaggctg tcgcggggcg cgtggatgag ggcggcgtcc ggctgaccga
    17461 cgccgaatgg aagactctgg ctcgcaacat tcgggccgtc tccgaggtgg ccgcctgggc
    17521 gggtctgcga tgcgtctttc atcaccatgt cggcacctac gtggagacgc cggcggaagt
    17581 ggaacgcctg ctcgatatga ccgacctggg cctatgcctg gatacaggac actacgtcta
    17641 cggaggcgga gacccggtgg aagcggtgcg caaattcggc aaacgcatcg agtatctcca
    17701 tttcaaggac gtcgatccca gccggctgcg ctccgcgaac caagcccggc tgagcttttt
    17761 ggacggcgtg cgcagcggcg ttttttgtga attgggctcg ggggctgtgc gttttcccga
    17821 gctgatcacc gaattgagca ggatcggcta cgacgggtgg gccgtcgtgg aacaagacgt
    17881 cgatgcgagc ggcgctcctg ccaggcccgg cgctaagacc ccgttcgaaa gcgctcgatc
    17941 gagccgcgaa tacttacgca ccaaactcgg catctgagtc ccgcgaagct cctggcaaac
    18001 aagatggcgc ccgttggctg acggccgaag gcggccgcat caccagaagg ttaccgcgcc
    18061 tcctaagcgc ttgtacgtcc gcagatcacc gggagtcgtg cctgccgaat aacctgggca
    18121 gtggctccga cgccgccagt ccaatggcat cgagattgcc acgggggagc cggagggtgg
    18181 caaatgaaac ggatcacagc tctcggtgcg atctctctat tcgcagttct ttcttcgctt
    18241 catgcgcaaa cctcgaagct cgcgatcaac ggcgggggcg gggtcaccct gcccgtaggc
    18301 gacactggcg cccggctgaa caccggctgg aacatcacgc taggcgcagg gtacaacttc
    18361 gtgccacgcc tcggcgtcat gggtgagttt acttacaatc gtttcgacat taaccaagct
    18421 acgctcaatg ctttgagtca gccggacggg aacttgcggc tctgggcatt cacagccgat
    18481 cctatcgtcc gactgaatcc gggcggccgc ctggatttct acgtaatagg cgggggtggc
    18541 atataccatc gcgtcgtaga atttacgcgg cctaccgtga ccacagtgac agtattcgat
    18601 cctttttttg gcttctacaa tgtatttgta ccggcgaacg aggttttgag ttccttcgcg
    18661 accacaaaag gcgggcttaa tatcggcggc gggatcgcct tccggctcgg cagcagccgc
    18721 gtcaaagtgt ataccgaggc ccgctatcac catatgttca cgcgaacggc gaccaccatt
    18781 ctgccggtga ctttcggctt gcgatggtag ggtatagccg gcggccggcc cgtcctcctg
    18841 cgcttatgaa gcggcactat ccgaagcggc ttcccccggc cgatgcagcg gactcagctt
    18901 ctctgccgag gcgattgggg agccgactag tcacatgacc tgatcggacg ggcggccggc
    18961 cagtgctgta tgatctacct tttcggcgag ctggcggagc ttcacctttg gcccgctacc
    19021 ggcccagaga agggatcgtg catcaacaac atggcttgtt gcaattgcac tccgatgata
    19081 tactcttggc ggtttcgagg ggccacgagg aaaagactgg gggtgagtgt gctcgatctg
    19141 ctgattctca gaattatttt tatcgcagca gtcggattaa cggcattatt tatcaagcct
    19201 tttgggctgc aatcatggtg gcaggccggc ggcatgggcc tgctgatggg cggggcgatt
    19261 atcttcttcg agattcgcct ccgccgcgct agtttgaaga ggctgatcgg cgcggccgtg
    19321 ggatcgctgt tcggaattct ggccgcacta ctgatgagcg tcgtcctgga tctaagtgga
    19381 gcgtttccct accagacgct ctcttttctc caccttggct tgctgctgct tacaagctat
    19441 gtggggctgg tggtaggcgc cagcaaaggc gacatgttaa acctggccgc gctcggcggg
    19501 cttttcggtt ccgaaaaaac ggccaacagg tcctccaaaa ttctggatac gagcgttatt
    19561 atcgacgggc gcatcgccga tatctgcgaa accagctttc tggacggcgt tttggtcatt
    19621 ccccaatttg tgctgcggga actgcaactg gtggcggatt ccgctgactc gttaaagaga
    19681 aaccgggggc ggcgggggct cgatgtgctg caacgcatcc agaagatgtc caacatccag
    19741 gtgcaaatcg tggaagacga ttttcctcac attcgggacg tggacatgaa gctgatcgag
    19801 ctcgccaagc tatatgacgg caaaatcgtt accaatgatt tcaacctgaa caaagtggcg
    19861 cagctccacg gcgttgaggt gctgaatatc aacgagctga ccaatgccct caagccgatt
    19921 gtccttcccg gcgaagtcat gcgggtgttc attttgaagg aagggaagga gtataaccag
    19981 ggcgtggcgt atctggatga cggcacgatg gtcgtcgtgg ataatgccaa gcgcatgatc
    20041 tcgaaaacta tcgacatttc ggtcaccagc gtattgcaga cgacagccgg taaaatgata
    20101 ttcggcaaat atgacgagcg gacacagcat accccgccca cgcaggggcc ggagcgcggg
    20161 tccggctcgg cagaccggcc cgaggctccc cgcggccgag aacggtccga acaggtggta
    20221 aatcactaat ttcatgaggg ttgcagccat cattcccgcc gccggcctcg gtacgcggat
    20281 gggccgtggc gtttccgctg gcagtccgcc agacaaaagc cttccggaaa aaagcggaac
    20341 cagccgaaag caattcatgc ttctagaagg ggcgccgatc ctggtacaca ccctgcggaa
    20401 gttcctggca tgtcccgccg ttgatcagat cgtggtggcc ctgcgggcgg aggatatgga
    20461 ctgggttcgc gaacaactgc ggcgtgaaaa tccatcgaaa tccgtagagc tggtgatcgg
    20521 gggcgagcat cgccagcaat ccgtacggca cggcctggct gccgtcggcc ctggaaccga
    20581 tctggtcgcg gtgcacgacg ccgtgcggcc gtttgtctcc atcgatctga taggaaaagt
    20641 aattgccgcc gccgaagaga cgggagcggc aatcctcgga atcccgccgg tggatacagt
    20701 caagcaggtg caccgcacga tcatccatag caccctcccg agagaacaca tcgtgctcgc
    20761 gcagactccg caggtgtttc gcgtggatct gctgcgccgg gcgtacgaac aggccgagaa
    20821 ggatggcttt gtgggcaccg atgaggcgag cctggttgag cacctggatg tccgcgtcac
    20881 cgttgtgctc gggaacgacc gcaacatcaa gatcaccaaa cccagcgaca tggcgctagc
    20941 gaggctcttc ctccaggagg agatggcgtc ggtctgacca ttttggctgc tcgctgcaag
    21001 accgaaggct ggcgctgacc tgccccccgc tcccacctgc ggaatctctg ctcaaaacgt
    21061 aaaaagctcc cgccaaattc gattgaggct ataaagcgcc ggtgctccga gctgatgcag
    21121 ccgagatggt atacccgcgg aagagctcgt tcgcgactta ctttttattg acatcccgcg
    21181 tttttcgagt agactaccta aaaattacgc tgatcgttga taacgggagg tccgaatgcg
    21241 gggaaacgct gctgcaaggc tttttcttgg actggtcttc tcagccgccg cctggtccca
    21301 gggcctgagc acaatcaacg gcacggtaac cgatcccagc ggggcggtga ttccgggagc
    21361 caggattgtg gtaagagaag tggaaacgag tcttagccgc gagacgatca gcaacgggga
    21421 tgggctttac gtgatcggct ctttgaggcc aactcggtat accctctcgg ccgaagcgca
    21481 agggttccgg cagttcacgc aaagcggaat cgttctcgaa gcgaacgaca ccgtgaccat
    21541 caacctcaag ctcgaggttg gctcgaccgc cgagacggtc aacgtcgaag cggcagccac
    21601 ccaggtggac acgacgacct ccaccctgcg ccaggtggtc gattccgcgc gcatgatcga
    21661 gcttccgctg aacggccgca atgcggcgca actcactgcc ttggtagcgg gcgccgtcaa
    21721 cgcgccgtcg aacaacgccg accaggggac gaccaaaacg tttcccgccg cggtgacggt
    21781 ctcggtcaac ggtggacgcc agaacaacat cgcctacaat ctcgacggag tgcacagcga
    21841 agacatattc agcaacgtga accagccgct gccgatgccg gacgctttgc aggaattcag
    21901 cttccagacg agcaatttca gcgcggaata cggccaaaac tcctcgggcg tcgtcaatgt
    21961 agtcaccaag tcgggcacga actccctcca cggagacgtt tttgagttcg tgcgcaacgc
    22021 ccggttcaac gcccgtaatt tttttgccgc caggcgcgac cagctcaagc gcaatcagtt
    22081 cggcgggacc atcggagggc ctattttgcg ggacaggctg tttttcttcg ggggatacca
    22141 gggcacccgg attcgcaaca cgcagggcgg cctgagcgcg tttgtcccta ccgccgccga
    22201 tctgaatggc gatttctccg cctacctcaa cgcgagcgac cccagcaacc cgctacatag
    22261 ggcgatttcg ctcaaggacc cggcaaccgg acagcccttc ccgggcaatc aaattccaac
    22321 cagccgtttc gaccctgccg cgctcgccat gctgaagtat cttccagcca gcgccagcgc
    22381 caacggcttg gtcttttaca gcacgcccgt gattcaaaat tttgatgagt ttctcatccg
    22441 cttggattac agcgtttccg ccagcgaccg cctcaatttc cgcttcaaca aagcttggta
    22501 caaccagccg ggaattttcg cgaacaacaa tctccttacc tacgccgatc acaccccgga
    22561 tagttcctac aacacggcga ttcaggaaac ccacgttttc agcccgaccc tgctcaatga
    22621 ttttcggttc ggagtaaccc gcgaaataac gagccgccat ccgccggaca acactccgaa
    22681 tgtccgcgac ttcggcgtgc agaacattta tcagacgccg aataaggcga tcgaatcttt
    22741 cagcgtctcc gggtacttca gttttggagc ctttgccgat tcgatcttcg cccgagccac
    22801 cttcgactgg tacgacacga tccgttgggt aaaagggcgt cacaattttg cgatcggcgg
    22861 cgcctttgaa cgggcgcgtt tcaatcagaa caatcatctt ttccaaaacg gtacattcag
    22921 cttcaccggc gatattagcg ggaatgctct ggctgatttt ctgctcggta gactgcgtac
    22981 ctttacgcaa ggctggggat catttcaacg ggaccgcaac attctctttt ctctgtttat
    23041 ccaggatacg tttaaggtta gttcccgcct cacgctcagc tacgggctgc gcttcgagcc
    23101 ttcctttccc tggcacgatc tttacaacca ggcagaagcc ttctcgccgg ctctgtatta
    23161 tcaaggcgtg aggtcgcagg tctataccaa tgcttatccc ggagagatct ttaccggtga
    23221 tcccgggttt cctccggacg gccgttcgaa ctcgtggaac aacctggcgc cgcggctcgg
    23281 ctttgcttac gatccatccg gcaacggcaa gaccagcata cgaggcggcg ccgggatttt
    23341 cctaaactcg cgcgtgccgg ctttttccaa cgatgccatg gtgcagacct cacccttcag
    23401 ccccacggtc agcctgatca atccaccttc attcagcaac ccttatcagg gagtcacgaa
    23461 tccctttccc ctgacgttcc ccgtgccgca cgactttgtg ttcccgacgc cggtccgtgt
    23521 atattcctgg gacgcctcgc actacaagct tcaaacgccg accgtctata actggaactt
    23581 gactatcgag cgtcaggtca ggcccaattg gctcctccgg gcggcataag cgtcacggac
    23641 caaccacatg aacgaaaacg agcagctgaa tccttccgtg tatattccgg gaagcagcct
    23701 gtccaccgac gcgcgccgcc ccttccaacc ctataccagc atcgtacagg ctaccggatc
    23761 cggaaatgcc tggtaccact ccatgcaatt gagcttggaa aaacgcctct cgcgcggatt
    23821 cacaatcctc gcaaattaca cctggtcgaa gagtctcgac aatatgccac agggcacgga
    23881 tgtgacttcg ccgatcctga acgctgtcat cggcatgtcg ccatacgtcc cacacttcaa
    23941 gtcgctggat acgggacctt cggattttga cttcaaccat gttttcgtgg tttcctacgt
    24001 ctggcaactg ccggctttgc agaattccag gcggtggctg cgggtggcgg cgggcaactg
    24061 gcagctcact ggaatcacca gcgctcagac cggcgggcct atcaccatca cagccggtca
    24121 ggaccgctca caaaccggaa tcggaaatga taagggccaa ctgttagcac agagcgtgta
    24181 cggctccggc gcctgcggaa cagccgcccc ttgtgtcgac tatcttgcgc ccactgcctt
    24241 tgggttgccc cctttgggga cgtatggaaa tatcgccaag ggaacgctgc ggggtccagg
    24301 gttgttcaac accgacctcg gggtgttcaa acgttttcca atcaatgagc gctggagcgt
    24361 gcagttccgc gccgaatttt tcaacgcctt caaccgggtt aacctgaata acccggtcac
    24421 ctcggtcagc ggcgccgggt tcggttccat tcgaggagcg cgcgatccgc gcatcggcca
    24481 gctggcattg aaggttttct tctagaagtc aatctctgcc cgccctataa gccgctagcg
    24541 cggggcgcga gcaaagcagc aactggggaa tgcgcgccgg gcgatcctag ggttggccat
    24601 ccccgcggtg ctattctgtc tttttgtctc agtagaattt tcgattctgc cgatctatgg
    24661 aatttcgcac cggcatcgga tacgatctcc atcagcttgt cgaaggccgc cctctcattc
    24721 tcggcggagt tgtgatccca cacaataagg gcctctcagg gcactccgat gccgacgtcg
    24781 tttcccacgc tgcgaccgat gccctgttgg gagcggcaac tctgggagac atcggggagc
    24841 atttcccgcc ttccgatccg aaatggaagg acgcatcgag cctgctattt ttgcaacggg
    24901 cgcgagaact gctccatcag agcggattcg agatcgtgca cctcgatatc gtgatcgtga
    24961 tcgagcagcc gaaaattctc tcctaccgcg gcaaaatccg cgagagcatc gccggggccc
    25021 tgggcatcga cccgcaggct gtctcattga aggcgaaaac cgccgagggg atcgggccga
    25081 tcggcgccgg ccaggccgcc gaagcccacg ccatcgctac ggtcagaaaa gcttagcgaa
    25141 gaccgggccg ccggcgctcc ccgaaagttt gcgcccagcg gttgtatgcc gcaagtccgg
    25201 tcatatatat aattcatacc tgctgagcca aatctcgagg gaaaggagtg tcgagcatgg
    25261 gttgcctact agaacgcgat ctattggagg tctgtggggg caatgtgctg tgtccctgct
    25321 ggatcgggga aaacttcgcc gggcgacgat tgagcgcgcc agcggcggta gttctgatcg
    25381 tcgctgccgc cggcgtcgtc ttgggcggga tccgagttta gtaaagcgga attttcagct
    25441 ttaagtttgt gccgtggccg ggctgcgatt cgagcacgac atcgccgccc agagcccgca
    25501 tgcgctgctt gatgctgttc ggtccgagcc gcagggtttc caactcgtcg agcgagtatt
    25561 ttccgccgaa gcgaaagccc ctcccattgt catttacccc gatgagaaga ctcgtgtcgc
    25621 ttttttcaag cgaaaacaaa acatgagtgg cttgagcgtg cttgtgaata ttgtgcagcg
    25681 cctcgcggat cacctgtaag acatcgaccg tgattttgcc cggcgtgcgc accggctcgc
    25741 tgccgctgat aaacgtcacc ggcacgccgc tttccttctg gaagtcgtcc accaggcggc
    25801 gcgcggcggc ggtcaggctg gtcgtatcca cctcgacggg gcgcatgctg ttcacaaatg
    25861 tgcgcatttc cgccacttgg cgtttggcga cctgctggag ctgctcgagc tcgcgcacgg
    25921 cggtctccgg gtcccgctca aggagcttgc gaatcgtgtg cacgcgcatt tggaagctga
    25981 tgacgctttg cagcggcccg tcgtgaaaat cctgcgcgat ccggtggcgt tcgcgttcgg
    26041 cggccgcatg ttccacggca tttatgacca gcaagtggcg gacgagctcg gtgaacgcgg
    26101 gcgccacgcg cctcaggcgc cgcagcagaa ggctgcgcat gggagaaata cgattgatca
    26161 gcaggagccg gccgatggct tttccctcgt gatacaagcc gcatccgacg gcattgtaga
    26221 tctgaaattt gcgaaccaga tgcatgtgca aatcgtagcc cggatgtccg gcggccgggg
    26281 gctcctgcct cgcagcctcg gctctccact ccgtgcggtc cggcgccgcc acggattcgg
    26341 ccaaaaatgt caattgctcg cccacaaaca cggcccattc ccggctctct tccaataccg
    26401 gggcgccggc cgcgttgcct tcctcgggag gcgggtaccg gcagagccag taatgatcgt
    26461 ttcgctggtc ccagaaagcg agaaaggcat gggagcagcg aaaccacgct gccagctctc
    26521 tcagcccatc cctgatagcc gctatggtat ccggctctgg agcgccgctg attttatttg
    26581 cgatcgccag gattcggcgt tcgaccagat actgctgatc ggaaaaaagg aacgccagcg
    26641 atccgcccac gaccagcaag aaaagcacgc tgtgaaccgt cacaatcagc ggcggcatgc
    26701 ggccggcggt agctcggact ccgtacacca ccagcgccag agcgcaggcc agcagaatca
    26761 cttcgcgcgc cccgtgcagc gaccagcttt caagcaccag gaaggcgaaa aatgcgccgg
    26821 ccagagccgc cgacattggg gtgatcgccc agaaaaccag cgatatcaga aagagaagat
    26881 ctaaaaacag aattggcaga gtgaacgtct tggccagcgt gtgggaatcg tagagcaaca
    26941 gcacgatact gtacgccacg aacgccagta cgatggccgc agccggagtt gtaaattcgg
    27001 gccgggatgt gaacgcttca gcggctagat aggtgagcgc aaggaaagcg cggtatacag
    27061 ccagccgcgc cgtgcggcgc cgcaatgcct gtgcatccgc ctggcccgtg ggcatcgttg
    27121 tcgccaccag catcgatttt accttgaaac gatttctttg gccgatgttt taggagccgc
    27181 ctacagagag cgctccgcca gctctccgat gagcggcaag tggtagcgct cctcgcggct
    27241 ggccttgatc agcatgaaaa tccagacgaa gacgatcaac agttgcagca tcgctcgaaa
    27301 cggaaagaag tgcatgggga cgagatggaa ccagggaaac cactcgagca gcagccaggc
    27361 cacaaaaaga tacagaccct ggaacgcgtg aaaccgaacg tcggcggcat ttcggaatcg
    27421 ttcggatgcc agcacaagaa ttgctgcaat ccatccagcc acgggaatat agcagagcat
    27481 ggctgccgtc cgggcgctga tgcctccgcc ttccgagggc gcggatggag cgccggccac
    27541 aggctggcgt gcgccgcacc ggccgcagaa gaaatcgcgg tcaccgagcg gcgttccgca
    27601 attcgtgcaa aagggcaact tgcttcctct cttctcttag agtacgtcaa tcggccaaaa
    27661 attgttcccg cgagcccgct ggaaggggcc tctgcgcaaa aaatgcgccc ccaaggagct
    27721 tcgcagcgtt tttcgctagg atggagggat gatttatccc gagatacgca acattcacag
    27781 tcccgatctg gagcccccga accttcccga ggacccgggc gattgcgaga tttcctttga
    27841 agtgttcata ggccccaagg atggcgaggg cgaagaggct ttccagttta ccgtcgtcac
    27901 ccccgcccgc ctcgcgcggg gaggcgaggc ccggtggggc cggggaaagc tcatccttcc
    27961 gtccttcgaa tgggctgcgg tagcccaatc cgtcgcgaag ctgctcgccc agtgtgggcg
    28021 gccgacctgg ggcgaggtgg cttccgaact aaacaaggag ctgttgtggg aatttgatgg
    28081 gcgccagccg caggtagtta actgataggg gtgctggaac ggttcggcgc tgtccctctt
    28141 cgctgcatgc gctgcacccg cctgttccgg aagcccgttc ctggctcgac cgagcgaacg
    28201 aaaaccgcat cgctccgggc gtaggaagat tttccggacg gcgttcgaaa aaccattcca
    28261 cccttgctat gtttcagcta caatccgagt tatgcccgcg ggcagagctc cttcaccaat
    28321 cctgccgggt aaagcggaag cctcgggtca caggcggagg ctcatcgctt tcggctggta
    28381 cggcggtaaa ttcaatcacc tgaaatggct gctgcccttg cttccaaagt gccaccacta
    28441 ttgcgagccg ttcggcggct cggcggccgt gttgctgaac cgcgagccgt ccccggtgga
    28501 gacctacaat gacatcgacg gcgaagcggt gaacttcttc cgcgtgctgc gcaatcaaac
    28561 tgatgagttg atccggttgg tcggtctgac gccattctcg agggaggaat tttacgtggc
    28621 ggtttcgccg gccgatcggc cgctgcccga ggtcgagagg gcccgccggt ttttcgtaag
    28681 agcacgccag gcccggacag gtctggccca aaccgcatcg ctcgggcgct gggcgaattg
    28741 caagaatacg agccgcgccg gtatgtcggg cgccgtatcc cggtggttgg gaagcgtcgc
    28801 cggcttgtcc gagattgcgc tgcgtctgct gcgagtgcaa atcgaaaaca ggccggcgga
    28861 ggaagtgatc cgggtctatg atgatcctcg cacgctgttt tactgcgatc cgccttacgt
    28921 tcacgagacg cgcggcgatg gcagcgctta ccggttcgag atggatgcag gcggacaccg
    28981 aaggctggcc gctgcgctgc aacggagccg gggaaaggtt gcggtctccg gttaccgctg
    29041 cgacctgatg gattttctat accggggctg gcggcgcttc gacgcgccgg cgaaacgctg
    29101 ccactctatc aaaaagatgc gccaggagtg cctctggatg aactactgag ggcttcgttg
    29161 caatggcctt ctgctgtgca atgggggcct caagccggca ggcaggaaag attgggccgc
    29221 tttgctccaa tgctttgctc taatacggat ggccgtgcgc gagagtccag gtgttcgggg
    29281 cgccgagccc ctggcgccgc gatctttgag gcggcgtttc gcttgtgaag cgctccgccg
    29341 ctggagccgg ttcgtgccca ttatggcgtt ccacccttat tacgaaggcc cgcgtcgatc
    29401 gaacgggtaa cggccgcagc gcccatcgcc gccgggaagc gcgaacagaa tcagacccac
    29461 tccagagcgc ccttacggaa aatccagatg taaccgacga tcagaatcgc gagaaacacc
    29521 agcatttcca ccaggccaaa caagccgagg acgctgtatt ggatcgccca cggaaacaag
    29581 aagatggtct cgacgtcgaa gatcacaaaa aggatggcga ctatatagaa gcgcactgtg
    29641 taacggcctc gggagtcgga agccgcctgg atgccgcatt cgtaagcttc gagcttctcc
    29701 ttaaacggaa actgcggccg cactagccgg gcgatgagaa tcgtcaccac gggaaacgca
    29761 atcgcaatcg ctaaaaagat caggatcggg atatactcgc gcggcataac gcatcaaata
    29821 tagcacatgc agatcagcgc cgctggagct gcgcccgcag ccgcttccaa aagcttgtgg
    29881 gctgcgctgg cggagccggc gcggccggct tcaccgcggg cgcgacagtc gccggcgcct
    29941 gtgccggctc ttcggcgtag agccggacaa tagcgccgag catgacgtag ctcctggtgt
    30001 agcgccggcc atcggatttt tcaacgacca acgaaaagcc gggaccggag ccgtatcggg
    30061 tcttcacgct gggagctacg gtaattggaa aatacccttt gatcctgcgt tcgatatagg
    30121 ccgtctcgta gcgatgccgg cggcgccccc agttgaacac gcggaaagaa tcgaaatcgt
    30181 tcggctcggt ggcgcggtcg atcgtagtcc acagccagat cttcttcacc tgcccgccgt
    30241 cagtaacctc ggcgagcgga aaataggatg tgatccggcg gccctcggca tactgcgcca
    30301 cttcatcggg aatgtcggcg tcgagcatgc gcgccagcgc ccatccggcg tgccccttgt
    30361 cgccggcatc aagggtccgc accagatacc actcgtcgta ttgtttgggt ttttcgggct
    30421 ggggagaagt cgacggagcg ccaccagcag ggggaggagg cgccggttct ggcgggatgg
    30481 cattcgcgct ttgagcggcg gctcgagcgg cgctgcctcc tggcgccgtc tgcgccggcg
    30541 gctcattatc gatcttgggg ccgcgtggct cgccggcctt tttgctcaag gccgcagccg
    30601 gcgccggctg cgccacgggc tcgggcggcg ccgctgtgcg ctctgtgacc cggcgcgcca
    30661 gcaagtctac tttttgatct tcgttaagtt gaaagaacgc cggggaccag cgatagggct
    30721 caagatggag gttcagcttg tcgcgagccc gatacacgcc cagctttggc agccctgctg
    30781 caaaacgggc caactcccca atctggttat gaacttcggg cgtgagcaat tggccctgtt
    30841 cggtccatcc ttcctggccc gtgggggccc gtactttcag gaagcggcgg cggcgctgca
    30901 cgatctccac ccggtctccg cgcttgagag tggcggcgat gccggaattc agagcgattt
    30961 ctctgcggag cggcagggtc tcgggagcta cataagcctc tccgatcgct ggagaccggc
    31021 gcgcctcgcg ggggtggcag gcgcagagcg cagctaaaac cgcgagcgcc aacccggagc
    31081 agcgccggtc acccaatgcc caggtcatcg gtgcgctcca gaggcgtgcc gcagttccgg
    31141 caaatgagtg aaccgcagtc gccgcagcgc agcggcttgc tgatctgccg atcgcattga
    31201 gggcagtagt attccgcgcg ctcgctgggt tgatcttccg gcatgcctgc cgctccgcgt
    31261 tacaagacaa caatggggtg gtaaagattg ggcagcttca cctccccgat ggctagcagc
    31321 cgcccctgca tatcgattgc tttgacgagc cgcgagcccg gccgcacccg gaagggagaa
    31381 actcgaaaat cgcggccgtg caaaatctgc gcggcggtga ttgcgtccac ctgctcggcc
    31441 ggcgcttccg gcagcaagtc cgcgccgcga attaatgcct cttcgagcct gttctgggcg
    31501 gcaagatcgc gaatctgctc cagggtgtgc gccatgtcga gcgtgaactc gccagccgcc
    31561 agccgcctga ggttttccac gtgcgccccc gtgccgattt tctgacccat atcgtgagcc
    31621 agcgcccgca gataggtgcc ggccgagcac cggatacgta ctttggcgcg cgaaccctcc
    31681 acgccaagca gagtgaattc atctacgcgc acccgcacgg gctccagctc gaccggacgc
    31741 agcttgcgag cgagcttata ggctgccacc ccgtttactt tcttggccga gatgggcgga
    31801 ggtatctggt caaattcacc gcggtagccg gcgagcaatt cttccagccg ctcagcggaa
    31861 agcgcgaccg gaacgtcggg tcctgcggga agtcccgcgc tgtcatacgt atcggtagcg
    31921 aacccgaagc ggatcaccgc ctcataggtc ttttcgcggt gcagaaaaaa ttgagcaagg
    31981 cgcgtggcgc ggcccaaggc tagcggcagc acgccggtgg caatagggtc gagcgtgcca
    32041 aggtgcccca ctctctgaat ccgtgccagc cggcgcacgc gattcaccac gtcgtgcgaa
    32101 gtgattccgg ccggcttgtc cactaccaga atgccgtcca cctttctatt ttagcgttcg
    32161 aatccgaccg agcgcgggtc ctgtagtgat gtggctcgat gctgctcagg actgaagtct
    32221 gactcgcaga agggccacca ggtcctccgg cagagaatcc ggcgccctga cgcggtagag
    32281 aggccggcgc tcgagcagtt ttgaagatac gctgtcccag gcgggacgaa attccgatgc
    32341 aatattggaa gcgcctcccg agccgggcgg gaggggcgcg aatgcggcgc tggttccggg
    32401 tggcctatcc agaataagaa aaacatcggc gcggtccaaa tggtcgcggg cgctcgcctt
    32461 gaaatcagaa acactcggat cgaggaccac gagataaagg agcggtttca agaaacgcag
    32521 tacgctgttc gactccatga ttacgcagcc cttgccgtcg atcagccggc gaatctcggg
    32581 catggctcgc gccaggccgc cttgcggcgt gcgaacccaa taggcttcgg cggcccccgc
    32641 cgccaggaag cgagaagtgt catggtggcc gtcgcggctt tcctcgcgcg taatcgagaa
    32701 cggatgagcc ggatcttcca gtatgcagtc gcagacgcgc ccgtccgctg agcagagatg
    32761 gtggccatat tgggtcagct tgagagcggt ccaccccagg tcacgtgtgg cggcgatcac
    32821 cgaagccgcc accgacgtct tgccgatatt acgcgagtgc ccgccgacaa cgaccagttt
    32881 catttttcgg tatccgcctg cgccgtcttc ttttccaggc tggaaatcaa tttcggcagc
    32941 cggtcaaaca gggcgaggcg ttccaaatac tgcttgagag gccgcgcggg agttccccag
    33001 acaggctctc cggcccggac gatcttggat gtaagaactc cgcagcccga gccgagcaca
    33061 gcgcggctct cgatgcgcgc tttgtcgccg atgccgacct ggccgccgat taccgcgtag
    33121 tcgccgattt cgactccccc ggagaggccg gtctgggcag cgattacgac gtgacggcca
    33181 atgcggcagt tgtggccgat gtgcacaaga ttgtcgagct tggttccatc tccaatccgc
    33241 gtgacaccca gggcggcgcg gtcgatgcaa gtattggcgc cgatctcgac atcgtcgccg
    33301 atttccacgg ttcccacctg aggaaatttc tcgtatcggt tgttctcgag cacaaaaccg
    33361 aacccgtcgg ccccaatgat gcagcccgaa tgcagcacgg cccgctgacc tatgcgcact
    33421 cccggataaa tcgtcacgcc gaaatgcagc aatgactctt ctccaatcac gacgccgtcg
    33481 ccgatcaccg atcttgcccc cacgatggcg cgcggcccga ttacggcgtt ctctccgatc
    33541 acagccagcg gcccaacgga gacgccatcc ccgaggcgag ccgtcgcggc cacgcaggca
    33601 gtgggatcaa ctcccggctt aaccggacgc ggcggatgga gcagagcgat cacgcgcgcg
    33661 aaggcgttgc ggggcgccgg aacgcgtatc accgtccgcc gctccctgtt gctgaaatcc
    33721 ggagccacca ggaggcaacc ggcgcgcgtc ggcggcatgc gtctgccgcg gcggctatct
    33781 acaaaggaaa tatcggcagg ccccgcgtcc tcgagggatt gaacgcgggc gatctgcatg
    33841 ctggaatcgc cctccaccgg cgcgccgagg gcgccggcga tttcgccgac ggtcatggac
    33901 attctctccc gggccgggca ggatttcccg tccctaccgt atcatacagt gaggccagcc
    33961 tgccggcttg ccgcgcgcag cctatgtctg tgattcctgg agcccgcatt gcgcctacgg
    34021 cgcgcgtgag cgaacgcgcc cagatcggcg aaggcgtcga aatcggggat ttttgcatca
    34081 tcgaggagga cgttgtcatc ggaccgcgca cgcggctaga accttatgtg atggtcaagc
    34141 ggtggacctc cctgggcgcc gacaatgaaa tttccgccgg gaccgtgctg gggacggatc
    34201 cgctggataa agcctttacc gggaagcgca gctatttgaa gatcggcaat ggcaacaaga
    34261 tccgcgagca ctacaccatc agccggggaa ccgaaccgga atcggtgacc gagatcggcg
    34321 acgacaacta catcatgact tccggccaca tcgcccacaa ctgcaagatc gggtcgcgta
    34381 cggtcatttg cagttgcgct ttggtcgccg gatatgtgga agtggaggac gaagcattca
    34441 tatcgggcgg agtggtgatt catcaatatt ccaagatcgg tcgtctggcc atgatcggcg
    34501 gcaatacgcg cgtgaatttg gacgttccgc cctacttcct gtacacggat ttcaatgtcg
    34561 cgccccgcgg cctcaacctg gtgggcctgc gccgggcggg gctgaaggag caggatgtgc
    34621 ggctgctcaa gcgcgcctat cagttactgt atcgctcggg actgaagctg gaggacgccc
    34681 tggaggcgat cgagcgcgag gccccgggcg agcacacgca gcacttggtg gacttcatcc
    34741 ggcggtcgaa acgcgggatt tgccgttaga gatctctgct gtgcggcgaa acggctgtcg
    34801 cccgggctac ccttctttct cggcccgctt cacgacaggc tcgagcatct gctccacggg
    34861 cgcgtagtca tcggtaagaa ccacattatg ggaacgctgc cgcagcgcgt tgaaatgctc
    34921 cggcttcagt tccgaggcgc ccacgccggg tttcaaggga ttgccttgca gcggctgcat
    34981 ggagccaacc acgacaaacg tgtcgcggcg gtccggctcc tcgctcggcc cgttttccac
    35041 tgtggaaaat gcgtagacgt ggggaaagct tttggcgaag gtattgagaa tgccgccgag
    35101 aaactggccg gaggcgtaaa tgtcgatgac gttgatcatg taaacgccgt tgggcgacat
    35161 cagctcgcgc agtttcagat tgaattccag ggtggtgagg tggtacggca ctgaatagtg
    35221 attgaaggcg tcgccgtaaa tcaggtcgaa agacggcacc cgctctcccg cgtgcttgcg
    35281 ccgcagcaaa tcgtccacgt ggttgcgggc gtccaggtta aagatatgca tcggcgtgtg
    35341 gcgaggcagc ccgaaagcgc ggaaagcggc ttccgtgacg cgagggtcga gctcggctac
    35401 ctcaacgtag ctctgcggcc atctcgacag cagatatcgc gggaacacaa agccgccgcc
    35461 gccgataaac agggctgagg cgccgcttcg gcttccgatg gcgtcctcag taatcgaggc
    35521 gtagactttt tcgtagtcgt attgcagatc gttgggatta tcgggaacgt aataggaatg
    35581 aatcaggtga tccagcgaaa ggctccgcgt gccgggggga tcgctttcct cttcgatctg
    35641 aatgaaggaa tattggctct cgtccgtgta aaggacgttg ctgtaccgct cacggcggaa
    35701 tccgattttc gtgccgaact tctgcgccca cggccatggc ccgagcgatg ctccgattaa
    35761 gcacagcgcc gcggcgcccc agaagtaggg gacaagcgtc cgcgcgccaa acaggaccgc
    35821 taccattgcc aaccccagcc ccgcgctgac aatcacggcg atggtcccca tcttggcgat
    35881 caggtaaaaa cccgtaagga aggtgcccac aatgctaccc agcgctcccc atgcataaac
    35941 gttgcccacg gtgcgtcctg tttcccggcc aacatccaac gccattttcg ccgccatggg
    36001 gccgatgcaa cccaaaacgg ccgacggcag gaaaaaaacc atgaagacat gcaaagcgat
    36061 acgagttgcc cactcctgcc gcaggagaaa tccccagttg ccggcgattg agttcaggcc
    36121 cggcaccgcg aagcagaata gcgacgccag cgaaaagaca atgcccaggg cgctggccgg
    36181 ccggtaacgg tcggccagcc gcccgccggc cgagttgcct atcgcaatgc cggcgagcac
    36241 cacgccgatg acggaggtcc aggtataaag ggaactcccc acgtggcgcg cgatcagccg
    36301 gcctgccaca agctcgacga ccatgatgca catgctcgag acgaacgcga cggcgtaggg
    36361 aaccgccatc gccgccgcgc cgagagacac gacgctcgcc cgcggcgcga gcgtgtcgcc
    36421 gctctctacc tgcgaagtag ccatgatgat ttctccaagg gggtagtata gctggaatca
    36481 agcggatttg cgaaaattca caggcgcaac ttgcgatata ctataccgcc ggaggaagat
    36541 catgcgccgc cgtgagagct gcgcgatcct cgccgcctca tttttggccc tagccgttca
    36601 gcgttcggcg cgaggacagc agtcgaaaga gcaaaaggac gaaaaacctg cccgcatcga
    36661 tattgagacg ctaaggaagc aggtggacgc gaagaaggat atgttcttca tcgacgtccg
    36721 cgagccgaaa gagatcgaag agttgggcag cctgaagggc tatgtaaaca ttccggtcgg
    36781 acagatcgag agccgcctga acgagatacc caaagaccgc tacatcgtca ctgcctgaaa
    36841 cggcggcgga cgcgctgccc gtgcggcaga aattcttcag aaacacggct acaagactgt
    36901 acaatattgc ggcttgaagg agtggaggga gaagaattat cccctcgtct accccaataa
    36961 gcagcagtcg gccaaataga atcctcggaa aagcgccgcc caggcgacgc ggcgccggcc
    37021 cgctttctaa cgggccgctt acaatcgtct ccaattccgg cgtagccttc cccccgaggc
    37081 gtcggccttc cggcgcgggc cgccaaagac caatgtgaat cggatggctt tccaacactg
    37141 caatgtgcga gccagactgc gggagcgtgg ctttttggcc ggctctttcg tcgagattgc
    37201 cgctcccgag atcgtcgaga ttctgggctc cgccggcatg gacttcgccg tcatcgactg
    37261 cgaacacgcc gccttaaggg ggccagcgct ggcgaacctc attcgcgctt cggcctccac
    37321 cggcatcgcg cccatagtgc gcgtgcggga aaaccggccg gcagagattc tcgaagcgct
    37381 cgatctcggg gctgctggac tgcacatccc gcaagttgcg acccgcgagc aggcggaagc
    37441 ggctgtgcgc gcggcacggt ttccgccggc cggcgaaaga ggctttaacc cgtttgtgcg
    37501 ggccgccgga tacgggagcg tcgaagttga ccagtttcgg cggcaaagcg acgaagaaac
    37561 gctccttgtc ctgcacgtcg aggggggcct ggccgtctct tgcgctgaag cgattgtctc
    37621 cgtgcccggc gttgatgtag cctttctcgg tccgtatgac ctctcgatgg ctctgggaat
    37681 ccccggtcaa gtgacggatg cccgcgtgct gcgcgctgca cgggctgtgc gcggcgctgc
    37741 cgagagatat ggcgtggcgt tgggcgcctt cgcgaattca gtcgagcatg cgcgggtgtg
    37801 gttaaatgaa ggggttcgtt acctggccta tggcgtcgat tcgcgtatgt ttctgggggt
    37861 cgccgccgag gttcgccgcc agattgatgc gatgaggccg caaaaatagg gcggcggccg
    37921 gagcattatt ccgactaccg cgaaaagtgc tgccgccatt cggcgcagga actggttttg
    37981 ggctattttc ccttgagggg agaagcaggc atggtgctga tcatattggc cgttttgctg
    38041 gctggggcta tgggagcaca gaatgcagct gctcaggaag tgacttttca gcggttggta
    38101 gccgccgaca aggagccgca aaactggctc agctatggcc gcacgtataa tgcatggcgt
    38161 tacagcccgc tggatcagat caaccggtcc aacattcgaa agctggtggc tgtgtggtca
    38221 ttccagactg gtgacgtcga aggcggtctg caatgcactc cgctggtggc cgacggcgtc
    38281 atgtatatca gcacctcctg gaaccgggtg tatgccctgg acgctgccac gggcaaaaag
    38341 ctttggcatt acgtttacga gaagccgaag caggttgccg cgcagttcgc gccttggaat
    38401 cgcggcgtgg ctctcggcta tgggctggtc ttcatgggca cgctcgacaa ccacctggtt
    38461 gcattggatg ccaaaacggg cagggaagtc tggaacgtca cgatcgagga tgccgggcaa
    38521 tgtggctgta atgtcaccgg cgctccgctg gtggtcaaag acaaagtcat tgtcggcgtg
    38581 accgggggcg atacggccca ccggggctat atcaacgctt ttgacgcaag gaccggccgc
    38641 cacgtgtggc gcttctgggt catccccgga ccgggagagc ctggcagcga aacctggaaa
    38701 ggcgatagct ggaagtttgg cggcggctcc tcctggatga ccggttccta cgatcctgag
    38761 ctgaacttgg tgtactgggg gaccggcaat ccggcggccg atttctacgg cgatgatcgg
    38821 aaaggagcca acctctatac cgactcgacc gtggccttgg atgccgacac tggcaagctc
    38881 aagtggtact ttcagcaaat tccgtatgat gtttgggact tcgatgccgt ttacgagaac
    38941 gtccttttag atcttccata caaaggacaa aaacgaaagc tgctcttgaa tgtaaacaag
    39001 ggcggctaca cgttcgttgt ggaccggacg aatggcaagt tcgtgtccgg ctggcctgtc
    39061 gtggagaact tgaactggat taaaggaacg gatgaagagg gcaatctggt ggggcgcaac
    39121 gagccggttc tgggaaaacc aaatttcatc tgcccagccg ttgctggagg gcggagctgg
    39181 aatcatgggg cctacagtcc caagaccggc tggttctaca ccaccggcct tgaatggtgc
    39241 gagagcctga cgcccatggt gcaaaaaccg ctggaaggca agccttttat ggctggagat
    39301 ttcgaaatga aacctccacc aaaaggcgaa cctggcggtc acatcggagc ttacgacccg
    39361 atcaccggca agcaacactg gatctaccgt tccaaatatc ctctgctggc ttccgccttg
    39421 gctaccgcgg gggacttgat tttcaccggc gatccggagg gccaattctt cgcgctggat
    39481 gcggttaccg gcaaaaagtt gtggagcttt aacacgggct ccggccaccg ggggtcgccg
    39541 atcacctatt ccgtcaaggg ccgccaatac attgccacgc caagcgggtg gggctcgtcg
    39601 ctggcgggtc tgatgccgca gttgtggccg gaaacggaag ccttccagcc cggatcggcc
    39661 atcttcgtgt ttgccttgca ggaatgaagc gctgggcgag tccgaggaag attgcgtccg
    39721 ggaaacccac atccctgcgc tggaagctgg cgtgtggagc gctcgcgggc tgcctgacgc
    39781 taagggctca ggcgcccaat ataaatctgg acgatccggc tgtcatcaac caaggccgcg
    39841 agcttttcgc tgccacctgc tcggtcggat actgccacgg aaaagaaggc cgcgccgggc
    39901 gggggccgcg tcttgcgggg cggaaattcg aacgcgacta cctgttccgg acaatcacaa
    39961 acggcatcaa caagtccctg atgccggcgt tcaaaagcca gctctcccaa gagcagatct
    40021 gggaggcggt ggcctacatc cagagccttt cgggcgatgc ttcgggctcc cccacgccga
    40081 aagcaggctc ggcatcggtc cccgccccct cggctgtgct ggccagacca ccgcaggttg
    40141 ccccggcgga tccgaatgcc ggagactccg aagccggtcg agctttgttt tttgacgtct
    40201 ccaatcccag gcattgcgcc gcctgtcacc agttccaagg aaagggcggg gatgtagggc
    40261 cggatctgac ctccgttgcg gcaagaccct cgaaggaaat cctgcgtgat attctaaacc
    40321 ccgatgcccg tttggccgcc gagcctgtca ccgtcgtcac caaatcgggg gagaggatca
    40381 ccggagtcaa aaagcaggag gtccgggacc gcctgcgcat ctatgacacg agcgcctcgc
    40441 cgccggttct gaggaccatt tacaaggatc agatccaatc cgtgatcccg gaaagggtct
    40501 cgcctatgcc gcatgattac gggcaaacgc tcacccgcaa gcagcttctg gacgttatct
    40561 cgtttttgaa atcagcttcc gtcagccccc gggaaatcga ataggaactt gggaaactgg
    40621 cagccgaacc ggggaatgca cgtcggcggc ggcccaacgg gtcttaaaat tcttggcagt
    40681 tgtttcgtgc gagagcatac tagaagaaac aacccctcgt ttggaggttg taaccaccat
    40741 ccggaggtgg atcatgcctg tcaggaaaat ccttcgtgcg attcgccgcg cgaagccgct
    40801 gacccgtgag gagggaatga acatcctcat gcgcgaccgc ttccagtgcc agtattgcgg
    40861 cttggacggc gccgccagtc tggaaaacgg tctgatgatg accgtggact tcatagtgcc
    40921 gcgcgctttg cgcggaaaaa aggacccgca aaatctggtc gccgcctgcc ggccttgcaa
    40981 tctgctgaag ggacggcgcc gcttcgccag tttcgaagag gccaagaaat acgtgctcgc
    41041 ccgccgggag gaacagcggc gcgaatggga gaaaaacgta gcgttgctgc ggcagcagaa
    41101 ggcgacagcc tgacaaggcg atcctttttg agaagcagcg gcttgcgcgc ggcgcgattc
    41161 gactgcggcg gaggggcctt gcgtgtgcac ctctgccccg cttgtgggaa atgaggcgca
    41221 tcatcaagca gcgtccaatg gcttggccgt gtggaaccgg gcgcgtttaa cagtcctgct
    41281 tgctaatacg ccggctttag gcggctgaat tcgaatttgg tagggtccag attgcgccgg
    41341 cggagcatct ggatgcattc ctcgacgctc tccccaaccg cttcgatgcg ccgtaggggc
    41401 agcgtcttcg gcgtatagat ttccaaagca atccagcttc ctgccagcgc cctgcgctcc
    41461 tcgatactca gatcgtcggg cgaacgcccg agaatggctt ccgcctgcgg cttctccatt
    41521 gttgccataa tttgaaaact cactttgcct cgggacgcgc ttccctgaga agcctcacgg
    41581 cccgatgatt cagtcacctg aattctccct tctcaatcaa atcctggccc gactggggac
    41641 gcctgccctg ggcggagggg cttgcgcccc agcctggtcc ggcgattcac aacccaagtt
    41701 gaaagcggaa ccccgcggct atcacctgct ctccagatac gcgaccaacg cgttgatctg
    41761 cgcatcattc attttcttga tgaatgccgg catcaactgg ccgccgaact tgatcttcac
    41821 tacaacgaaa tcgcgcgtcg gcttgccccc ggagagcggc agcttttcgt tcttgaacag
    41881 gtgattcaga ctcggcccga tttttcgcgt ctgcttctgg tctttatcta tatcgtggca
    41941 cactgcgcaa ttttcgcgat acaggtcctg gcccatccgg ctatgctccc gccagggcgg
    42001 caatttgtgg tctgccgcgg ctgctccggc agcgaacaac aagaccacgg cccatggcgg
    42061 agctctcatg agcgctcctc gagtttctcg accttgctcg ctccagcttg atagaggcat
    42121 tcgccggtcg ccagcatctg gctgggctcg cagcgaaccc gcagatacaa cactcccgaa
    42181 gcaagtttcg ggacaggagc gcgtttctca cgcttcagga tccccgattc ccagagaaac
    42241 gtcaggaaac aacccgccac tgcgcccagc atcgtgaatt caaagtgaat cacacctgtt
    42301 gcccacgggg aaaagtacgg catgccgccc gtgacgatct cgtaattgtg ctgggtccaa
    42361 tacacaaaga gcacggacag caggccggcg cccgcgccgc cgcacacggt cagcagggac
    42421 attcggctcg gacggtccag cagcccagct ggaagatcca ccggctcggt ggaaaagacg
    42481 tccaggtcgg ccgcatctgc accgcgcatc tttagcgcct ggatggcccc cgccagcgta
    42541 aactgatcgc tgaactcact catcaggtac atacttatat tcccagatgg cgatgatcgg
    42601 aaacagcttt gcaaaaatca ggtaaagcag cgccatggcg gcgaaggtcg agatggcgat
    42661 cgagatctcg acccatgtcg cgttgtagcc ggtgccccag gcagaaggca gcggcggaaa
    42721 cgacagggtt ggaatcacaa tgagaaaccg ctccagccac atgcctgcca gaacgcaaac
    42781 cgccgagatc acggccgttt tgatgctccg caggcgccga atccccacaa gaacgcaagg
    42841 cacaatgaaa ttgcaaatca ccatcgacca gaagaaaggc gcatagcggc cccgtacccg
    42901 ggcgccgaac accgccattt catggggctg gttcccatac cagacggtga ggttttcggc
    42961 cactgtaaaa taggcccaca gcagactcat caggaacaaa agcttggcca gattttcaaa
    43021 gtgcctcggc tggaggtacg cttgcagccc catcgttttc cggataacgg ccataatgag
    43081 cagcaatgcc gcgatgccgc tgaagatagc cccgaccaca aaatacggtc cgaagatcgt
    43141 gctgtgccac atgggagcga gagccatggc gaaatcccag gcgacaactg tgtgcacgga
    43201 gacggccact gccaggatca tgccggccat cagcttcata gccttctcga gagcgtgcca
    43261 ctcgcggtcg ctcccggtcc agccgagcga gagcgtccga taaagaagcc ctcgcgcgcc
    43321 gctggtatgc agtcccaact gtccgagatc gggaatcatc ggcagataga ggaacagcat
    43381 gctgccggtc agataggtga ggatcgccgt caggtcccac agcaacgggg agcggaagtt
    43441 cggccaaatc agacgcgaat tagggtaggg aatcagccaa taaaaaagcc acggccgtcc
    43501 gaggtgaata atcgggaata agccgccgat catcagacag aacacggtga tcgcttcggc
    43561 ggcgcgggtt accggttttc gccaggcggc gtgggtaagc cggagaatgg cggaaatcag
    43621 cgtgccggcg tgggagatgc cgatccaaaa aacgaaattg aggatataca gcgcccacat
    43681 ggtggggcgc gttttgccca ccactcccag ccctttagag atctgatagg cccagcaaat
    43741 caggccccag gccgtcacgg agccggtcag caggacggct accacgtact gcggactagt
    43801 gttgcgcagc gtgcgccacg tatcttcctg gatctggtcg tgcggaaggg tccgcagcgt
    43861 ggttgccatt tctctgctat ttcgcccgct gtaaataaaa caccttgggc tttgtgccca
    43921 agtcctcgag caggcgcgtc gccctgccac tggcagcgag gcgggagact ttgctttccg
    43981 ggtcgttcaa gtccccgaag accatcgcct cggcggggca ggattgcaca caagccggct
    44041 tgacttcccc gtccgccagc ggccgcccgt cggccgcggc gtccagcttc gcccgctgga
    44101 ttctctggat gcaaaacgtg catttttcca tcaccccgac catgcgcacg gcaacgtcgg
    44161 ggttgtgctg caactcgagc ggggcgtccc actgcggcgg aaagaaattg aaaaaccgaa
    44221 ccgaatacgg gcagttgttg ccgcaatacc gcgtaccgac gcagcggttg taaacctgaa
    44281 cgttcagccc ttcgggattg cggtaggtgg cgtagaccgg gcagaccggt tcacagggag
    44341 cttcgtcgca ctgctggcaa agcacaggca tgtatttcac ttttacgtcg gggaactcgc
    44401 cttcgtaata gcggtccacg cgaatccagt gaacggtgcg ccctcgcgcc gcctcagtgg
    44461 gattccatac gggaagattg ttttcggcgt ggcaggcgac tacgcaagct tcgcacccgg
    44521 tgcagcggtc caggtcgatc gccatacccc agcggtggtt ggtcgttgcc gcttgttgtt
    44581 ccggcactat ctgtaccccc aaggcccctg ttcccggtcg ttcgccgaga actgaatgag
    44641 ctgccgctca ggacccagtt tcgacagccg cacgcgcgtg gcgcccagcg ctagcgctcc
    44701 tgttgtcttc tcccaaaccg gcgccaaaat agacagcgga ttggatccac gccccgcgta
    44761 gcgcccatag cacttatgcc cgccgccgat cgccatgctc acgacgcccg gtatggccgc
    44821 cggatgtaca taggcgggcg catcaatatg cccgtgagga gactcgaccc ttaccagatc
    44881 cccgttttgt atccccaagg aggctgcggt cctggtatcg atttccaccg gcaaccccca
    44941 cattgcgctc gagaccgggt cgggcagttc ctgcatccac ggcaggttgg ctccgcgccc
    45001 gtcttggaac tgcaatgaga ggtaaggctg aaagtatagc ggaaactgcg ccgcctcgcc
    45061 agaaaacacg gcaggagtgc tgtcgatgac cggcccggct gcattggccg tgggagagga
    45121 agcccgggaa cgggccgggc ggctgcccgg cgaggaatcc gatccgggct cgccccagaa
    45181 gccgccctgc tccactacgt cgtcccatga ctgcccttgc ggcagaatcc tttcggccac
    45241 cgacctcggc ggcgccggct ggaaagacag gcccattttt gcggctatcg cagctaagac
    45301 ctgctcggtc gcgcgcgtgt cgtaaagcgg ctgcacaaac tgtgtcgtga ccacaaccgc
    45361 cgggccggga gctatcagag gaaacacgac gttggatgct tccaggggat gatgatccgg
    45421 caacagcagg tccacataag cagcgctgtc gtccaggaaa ggggaaaaac tcaccgatgt
    45481 cggaatgcgc ccgagcttct ccgcgatacc agcggaagct ggaagcgtat acaccgggtt
    45541 gcttccatcg agaaacaggt attgggcgcg ttccagcaga ggaatggcgt tggaaaaagc
    45601 ggcgcggttc tccaccgcgc ctggcgcggg cggccaaacg ccgcccggtc ttcccacatt
    45661 gcccagcatc acgttcaggt aagcagccgc cacgagcgcc gcgagggaat tggtttgcgg
    45721 cgccgaagcg cctgatatca ccagcggcgc ttcggactcc cccagctccc gggccagacg
    45781 gcgcacgcgc cgctcatcga gcccgcaggc gcgcgccgca gaggctgggt ccaccgactc
    45841 gaaagtctgg cggaccgggt cgggcagttc ctccggctgg cgggccatct tttcgctgag
    45901 cagcaaatgg ccaatggcga gcgcgaaaga caattccgat cctgggagga gcggcgtcca
    45961 ttcatcggcg ctctgtgcgg tgatggaaaa ccgggattgc gcctgaacca gccggccccg
    46021 cacggcggat cggccctgcc gaaaatgtcc gaactggcgc gcataaaaaa caggcgaggt
    46081 ccacccgcca agaaaatccg ccccgattcc cagtgcatag cgggcgttgg aaagatcata
    46141 gacgggcaac ccatcccacc cataaacctg ctccgccgct ttccgttcca gcgggaaatc
    46201 caccagctcg aaacaagccg gcggcggcgc tcccagagat tccaaaaacc gtgcgatggc
    46261 cagcgcacgg gcgccgggct ggggtccggt caaaaagacg atctgcgatg ggttttcctg
    46321 gcgaacccgg tccagctttt ccgcagcgac ggcaatggcc tcgtcccagg aggtggctat
    46381 gaaatcagcc tggccgcgag ggcctttccg gtgcatcggg ccgcgcaggc ggtccgggtt
    46441 gtaaagcgat tgcacgccgg cttgccctct ggcgcaaaga cggccgccgc tgaccgaatc
    46501 gagcggattg ccttcaatct tcttgactgc tgcgatcttc tggcggaact tttctccatt
    46561 gcgctcgatg acacgctcgc tttccatcac gcgcacgatc acgccgcagc cggacgggca
    46621 ttctccgcac aacgccggat accattgctc cgcgccggga acaatctcct gatccggcac
    46681 gagcaaggga atatacgtat ccagcttctt gccgcatgcg cccgtcatca gaccggctga
    46741 agcggtcgaa aagattttga agaaatcgcg ccgatccatg ctgtctcaat aatgacaggt
    46801 ggcgcagtcc tggctggcgc cgtgctgccg atggcaggtg atgcacgtac ccatgttgtg
    46861 tttcaccgca agtacagcca cagtcgcttt agcgacgtcc ccgtgacacg ttgtacaatc
    46921 gatcttggac cggtagtggg gcgcatgccg gaacttcacc agcgcttccg gactgaaccc
    46981 gtaaacgcgc tcccagggga tttccacttt tcgagccgca tactcgcgga gcttcttgac
    47041 gcctggcttg tcggtggcta acttgtcgtg acagagcatg catttgttta ccgaagggat
    47101 tccggcggcg gcgcgctcat ccgcgccgga gtggcaatcg agacaagcca atcccagcgc
    47161 catatgcctg ttatgaggaa acaggatcgg ctgctcggct aaagcggcag ccgcgcaaag
    47221 gctgatcaac catcgatagt tcatgcagac tgagaattat acagatattc caagatacaa
    47281 tgactgcaaa cggaaagaaa cggctgttcg cgataatttt gacggccatc gctgcggggg
    47341 tcggctatac tctctggcgc tcggggattt tccaccagat tacaaacaaa gacgaactgg
    47401 tccagtcgct ccgccgtgaa gggcttgccg gaccgttgct gtgcatcggc ttgcaatttg
    47461 cccaggtggt gatttttgtc attccgggcg aaatcacgca gttcgccgcc ggctacgtct
    47521 ttggcgcggg cctgggattt gtctactcca tcctggggat catgctggga tcggctttta
    47581 acttttattt tgcccgggta tttggccgcc ccgtggtcga gaagttcatc cgtcccgaga
    47641 cgctcaataa gatcgacaac gcgctgaaca acgccaaggg aaaatcagcc ctgtttctgc
    47701 tgtttctgtt gcccggcttg ccgaaagacg ccatgtcgta cggagctggc ctgtcgcgga
    47761 ttacactggc ggagtttgtt ttcatcagcg ggctgggcag gacgccggcg ctggtgctga
    47821 gcgttcttct gggatcccag gcatacaatc aggactaccg ccggatggtg atcaccgcta
    47881 tcgcggctgg tgcggcgatc ggcgtttaca tcctctatgc ccgccgccgg cggcagcagc
    47941 ctacttgaag caagagcagg cgcaacttgc gcgggctccc tgcggccggc gggaggggta
    48001 cactggaagg tggtatttct ttcttgcccc gaaagcgcta cgagtggcgg ttgcagaacc
    48061 ggacggtgat cctgggcgtc cgcacccttc tcatgggtgt attaaatgtc acacccgatt
    48121 ccttcagcga cggcggccgc tacctggatc ccgacgctgc gtacgcacgg gcgctcgaaa
    48181 tggaagagca aggagccgat ctgatcgaca tcggcggcga gtccaccagg cccggctcac
    48241 gccgcatccc ggcccgggag gaactgggcc gcatcgcccc cgtccttaag aaactccgca
    48301 acaagctgaa cataccgatc tccgtagaca cctacaaagc ggaagtggcg gagcgcgccc
    48361 tggagttggg ggcggagatc atcaacgacg tcagtgggct gacctttgac ccggctctgg
    48421 ctgaagtaat caaccgccgc gatgccgggc tcgtgctgat gcatattcgc ggcactcccg
    48481 agacgtggtc gcaattgggc ccgctgccgg acgtgatggg aaccatcgcg cgcgatctgg
    48541 atgcggcctt gtgccgtgcc cggcgcaccg gaatcgaccg gcgccgcatc gtgattgatc
    48601 cgggcatcgg cttcggcaag cgcggcgatc aaaactacga aatcattgtc agcctgggcc
    48661 gcctcgccgt gctcgatcag ccgattctgg tggggacgtc ccggaaatcg tttctcggcc
    48721 atccggcgcg cgaccgcgag gatctgcgcc tgtttgggac
//
LOCUS       DASREK010000165        13534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2062173, whole genome shotgun sequence.
ACCESSION   DASREK010000165 DASREK010000000
VERSION     DASREK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13534
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062173"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="sucC"
                     /locus_tag="VFA54_08525"
     CDS             complement(<1..1040)
                     /gene="sucC"
                     /locus_tag="VFA54_08525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZS50890.1"
                     /translation="MKIHEYQAKEILRRYQVPVPRGKAAFTADEAYEAARELGGGVVV
                     KAQIHAGGRGKGGGVRLAANAEAARQIASSMLGMRLVTHQTGPEGRIVQRLLIEETLP
                     IERELYLGIVLDRNLGKTVFMASRAGGVEIEEIARESPELILKQIIEPGAGLLAYQAR
                     ALAFDMGIPAAAVPSAAAAMAALARAYEETDASLAEINPFILTKDGRACALDAKMNFD
                     DNALYRHPEILALRDVNEEDPLEVEAGRYGLNYIKLDGNVGCMVNGAGLAMATMDIIK
                     YAGGNPANFLDVGGGASAEQVKNAFRILLSDPNVKAVLINIFGGILRVDTLATGVVEA
                     ARALDVKVPIVLR"
     gene            1288..2319
                     /gene="trpD"
                     /locus_tag="VFA54_08530"
     CDS             1288..2319
                     /gene="trpD"
                     /locus_tag="VFA54_08530"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZS50891.1"
                     /translation="MSAILEAVHKAASHKDLSSGEAFAAVEEILYGKAPESLVSALLV
                     ALRMKGETVDEIVGFARALRRHAVRVECAPDARPLVDTCGTGGDGAGTFNISTAAAFV
                     AAGAGARVAKHGNRSISSCCGSADVLEELGARIILSPAGMASCVREVGIGFLFAPALH
                     PAMAHVQPVRAALKMRTVFNLLGPLANPAGAEVQIVGVFERRLVVLLAKALAQMGLRR
                     GLVVHGSDGLDEITVTGPTEAAEIVEGRVIERTLLPEEFGLQRRPAYQIAGGGRQRNA
                     AIVRAVLSGEEGPHRDIVVANAAAALVMSGIAPDFPAGAAAARDSIDSGKAAETLGRF
                     IRFTQAQSG"
     gene            complement(2331..3686)
                     /locus_tag="VFA54_08535"
     CDS             complement(2331..3686)
                     /locus_tag="VFA54_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS50892.1"
                     /translation="MTSTLTAEQIATRLERAPVSMWHVQARVLVGIATFFDGVDALTI
                     AYVLPALIGPWHISPRSVGLLISAGYAGQLVGAIFFGWLAERVGRLAALSCTILVYSV
                     CSLLCMVSWSYSSLLIFRTLQGLGLGGEVPIAAAYINELAAAKTRGRFVLTYELVFPA
                     GRVAAALLGVSIVARLGWRYMFLIGGLPALLIFGLRRALPESPRWLASQGRLREADWA
                     VQQIEQGALWRNGQLAQHNAAAGGERMRAKAEWADLFRGIYRWRTLLAWVLWFSAYLV
                     LNGLSTWVPTLYRTVFRTPIQTSLRYGLFTSAAGLVGCVAVALLVDRTGRRTWFAASF
                     LLGGLAFLALWLTGASTPITVLSYTCAGSFFANSIAMLLFLYTPEIYPTRIRALGSSV
                     ASAWLRLASIIGPLLIGYTIGHYSLALVFLEFGCVCLAAFGASLLCVETGGWRLEKIS
                     P"
     gene            4167..5414
                     /locus_tag="VFA54_08540"
     CDS             4167..5414
                     /locus_tag="VFA54_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZS50893.1"
                     /translation="MTRRNLLSTAALAAPAAVDALANVPQSDRPRSQGKPSKITRIEA
                     TLLQKPLKERFWMSMNPIGGYNPRASRLILKMHTDAGVTGYGEGGGGGAEMLRKGFAE
                     LVIGEDPFMVGKIWEKMFGVTSTREMAVKGWSRGGVIGVMAAIDAAIYDVMAKSTGLP
                     IYKFLGAYRNTVPVYITGGYYREGKGIPELVQEVRGYVGEGYNALKLKVGGVSAGFSP
                     EDDYNRVKAVRDAVGPNVKIMLDVNNGWDLHTAIRASNKLADLDITWLEEPIHWYDDV
                     EPLKQLKLNTRIPIASGEQELTRWSARRLIETGAIDFMQFDCHAHAGITEWRKIAGMA
                     SMCHVQMAPHHEPIVHGHLLASIPNGYILEGFANPDRDPFWFEIYDKRPKIEKSMLYL
                     DDTPGWGIEFRQKALDQYGTKVI"
     gene            5732..6226
                     /locus_tag="VFA54_08545"
     CDS             5732..6226
                     /locus_tag="VFA54_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZS50894.1"
                     /translation="MSSVKTPISRRRLLARGFSLIELLIVIAIILIIAAIAMPKLTKA
                     RMFAYEMAAIRTLHTINTAQAQYFSTYGRYATTLAELGPPPGGSSVATAAAADLIPGD
                     LALGTKSGYNFTMVGTQQGYTANANPQVYNTTGSRSFYTDQSTVIREHLGNEPASAAD
                     PEIK"
     gene            complement(6427..8100)
                     /locus_tag="VFA54_08550"
     CDS             complement(6427..8100)
                     /locus_tag="VFA54_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50895.1"
                     /translation="MKTGKIFAILLVAAAALQAQPVINADGVVSNASYLPSGFPNSGI
                     AEGSIFAVFGKGMGPSTLAQANSFPLSTTLAGTSIKVTVAGTTVNAIMLYTVDGQVGA
                     ILPSGTPVGDGTLTVTYNGQTSAAVPIHVVRSAPGIFAVNQAGSGPGVITDVNFFTNL
                     ITASAAPGQVMILWATGLGPVSGDEAAGPLPGVAPSGVTVEVFVGGKPAKIVIFGRSG
                     CCSGLDQVAFEVPQGITGCYVPVLLRVNGVVSNSVTISISSSGKLCSDSNGFTADDLQ
                     KIANGGGSISAGSVLLTRSSISITAPPPVGNLVTTIDAGSGAFVRYDFSKLITSTGAS
                     GYAPYGSCYIYQYRGNSTNIPNPVTFTYLDAGASLTVTGPNGSKQIPKTSTGGVVAYS
                     AQLGGGTVGLPGFTPLYLTKGTYTIDNGAGGKDVGAFRATLTLPDPLTWTNKDSVTNV
                     LRSQDLLVTWSPTNLNGFVIITGGSANTNPQVSGAFICTERASAGQFSVPSVVLSALP
                     ASSTLSGVPAGFLSIGYSSDYQSGRFQATGIDVGYAIYFDSDGKNVNFQ"
     gene            complement(8362..9636)
                     /locus_tag="VFA54_08555"
     CDS             complement(8362..9636)
                     /locus_tag="VFA54_08555"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS50896.1"
                     /translation="MRATRVRWVILSLLFLATTVNYLDRIVFAVLIPAIRKDLRLNDL
                     EYGYINGAFQLAYTIGFLFMGKFIDWAGTKIGYLVSILWWSMAAALHSVAGSGIALGL
                     WRFMLGLGESGNFPSAIKSVAEWFPKKDRAFATGIFNAGTNVAATVGPPVIVAVTAVC
                     GWRAAFLMTAGLGLFWVPLWWRYYDRPEKHRKIDRAELEYIQSGREEAAPAVPWVAAL
                     RHRETWGFAAGKFLTDPVWWFYLTWLPPYFYDARHFSLMQIGWALPAIYLAADFGSVG
                     GGWISRFLIARGWPVGRARKGAMLLAAACMPLAVMAVFVGNPVGAIALISVATSAHQG
                     WSANLYTTVSDCFPSGAVASVTGIGASAGGLGGFLFSAILPGVVITYFGYVPVFILMG
                     ILHPLAWFCVHLLMGRIEPVSAGKQFVPAPAR"
     gene            9771..10994
                     /gene="ispG"
                     /locus_tag="VFA54_08560"
                     /gene_synonym="gcpE"
     CDS             9771..10994
                     /gene="ispG"
                     /locus_tag="VFA54_08560"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZS50897.1"
                     /translation="MNRRPSVVTDVGGVKVGGTHAIVVQSMTNTDTADVTATVNQVMA
                     LAAAGSELVRVTVNHDKAAEAVPRIVDTLEKFGVRVPIIGDFHYNGHILLKKYPECAR
                     RLAKYRINPGNVNIGHKHDNNFRTMIDVAIEHRKPVRIGVNWGSLDQVLLTRLMDENS
                     RLPEPRDAAEVTTEAIVQSALNSARAAEEYGLPHDSIILSAKVSRVPDLVRVYRELAA
                     RCDYPLHLGLTEAGMGAKGIVATAAALAILLHEGIGDTIRASLTPMPDGDRTEEVRIS
                     QQILQSLEIRSFTPQVTACPGCGRTTSTFFQEMAGQIQKYLREQMPVWRERYPGVEEL
                     KVAVMGCVVNGPGESKHADIGISLPGTFEEPKAPVYVDGRLVVTLKGADIVPEFIQIL
                     NHYVEARYTAVPASR"
     gene            complement(10998..12707)
                     /locus_tag="VFA54_08565"
     CDS             complement(10998..12707)
                     /locus_tag="VFA54_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50898.1"
                     /translation="MSTIFPEAMIDPISGKANRILALFSLLVGISFSFGHLAGMYYCG
                     RSYTPLVYDQWMVRGVGSPKYFYALDHVAWDETAAYARYAQEVLRGDFLGATLGSFEP
                     YVKGEARAQSIWCRDRLGPILLAALSAPFGRSVPKAFVAADFLFPALTAFCLLLLCRQ
                     LFPCLLLWLFAVSLAVWFSFFDLIGLLYLYWGDPHYGPIFARTAYPQISCCFFALFLI
                     AMLRLDRRRSIVSAAFLGFALLLNFYTYVYSWTLALAMLIVWLILLGSGLPFAGAENA
                     GRRLLAAFIAVPAGLAAAASIPVWLPLAARPAAVQDFFVRLGGTITRQPDWLALLFCS
                     LFAALALGAGRRGWRNGWFWAVFWIACFAVTNEQVISGRTMQPFHYLLFFMGPFFPIF
                     LCDIGYWRWSQSGLGKVLWRRAGLIVAGTVALGFAQCAFRLAQPVARERELHTTDRSV
                     DQVLAVLSMPGLRHEGFLTNDPLLDQILPAYVPQKPLQPWWMDPLGDSEIRALHLAAA
                     HLLEEPIDYRAAHPGPSLRFDPAKTIVVCNRHRPIRIRAAACQVLVSNDDFLVISLAP
                     CGG"
     gene            complement(12676..>13534)
                     /locus_tag="VFA54_08570"
     CDS             complement(12676..>13534)
                     /locus_tag="VFA54_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50899.1"
                     /translation="LGQYAESAIEVGTLHGRIFLPAPTLIRGLLSVFALNARAHKAYL
                     LGRVSSSSFPFYFLVALAVKTTLPLLLLSAIALALCVVESWTDSLSTAAYPAIAVALM
                     LAVGAMSRINVGIRHLLPIFPFLALLAASLFADCSTSSRPGGHILAGLAWSLLAWHGV
                     ESIAAHPDYLAYFNQIARGREEHFLLDSNLDWGQDLERLRQFARQHGISTLYLSYFGR
                     PGPDFLEIPDVRSLPAGMRPSGWVAVSKNHMGGIGRSPSDLAWLAGHTPAARVGHSIL
                     VYHFPGSHD"
BASE COUNT         2990 a         4159 c         4136 g         2249 t
ORIGIN      
        1 cggagcacga tcgggacctt gacgtcgagc gcccgcgccg cctcgaccac gccggtggcg
       61 agcgtatcca cgcgaagaat gccgccgaaa atgttaatca acaccgcctt gacattggga
      121 tcggagagca gaatgcgaaa cgcattcttc acctgctcgg cgctggcgcc gccgcccacg
      181 tccaaaaaat tcgccgggtt cccgccggcg tacttgataa tgtccatcgt cgccatggcc
      241 aggcccgcgc cattgaccat gcatccgaca tttccgtcca gcttgatata gttcagaccg
      301 tagcggccag cctcgacttc cagaggatct tcttcattca cgtcgcgcag ggccagaatc
      361 tccggatggc ggtaaagcgc attatcatcg aaattcatct tggcatcgag ggcgcaggcc
      421 cggccgtcct tagtgagaat aaacggattg atctcggcca gtgaagcatc ggtctcttcg
      481 tacgcgcggg ccagcgccgc catggcggcc gccgcggaag ggacggccgc cgccggaatg
      541 cccatatcga aggccagagc gcgggcctgg taggccagca gaccggcgcc gggctcgatg
      601 atttgtttaa gaatcaattc cggactctcc cgggcaatct cttcgatctc aactcctccc
      661 gcccgcgagg ccataaacac tgtcttgccg agattccggt cgagcacaat ccccaggtaa
      721 agttctcgct cgataggaag ggtttcttcg atcagaagtc tttggacgat gcggccctcg
      781 ggcccggtct ggtgcgtcac gagcctcatg ccgagcatgc tggaagcgat ctggcgcgcg
      841 gcttcggcgt tggcggccag cctgacgccc cctcctttgc cccggccgcc ggcatgaatc
      901 tgcgccttga ccactacgcc gccgcccagt tccctggctg cctcgtaagc ctcgtctgcg
      961 gtgaacgccg ccttcccgcg gggaaccgga acctgataac ggcgcaaaat ctctttagcc
     1021 tgatactcgt gaattttcat ctggaaagcg cctccgctat gcggcggcgt aagatagtaa
     1081 gctggctttc agccgatcgg cggcaacgcg caaacaggga gccgcgccgg ccgcaggccg
     1141 ctgaaagcgg cgtttgcaaa acccgcttgc tgcgcgcggc ctgacggaat cacaggtgct
     1201 gctttgggcc gcggactcta agaagcgata gaacgctttt ttcagcagcc tgcggaaggc
     1261 gactgaaagc gaattttcaa atataacatg tctgcgatcc tggaagccgt ccataaggcc
     1321 gctagccaca aagatctgag cagcggcgaa gcttttgccg ccgtcgagga gatcctttat
     1381 ggcaaggcgc cggaatccct ggtctcggcc cttttggtcg ccttgcgcat gaaaggcgag
     1441 acggtggatg agatcgtcgg attcgcccgg gccctcaggc gccacgctgt gcgggtcgag
     1501 tgcgcgcccg atgcgcgccc gctggtcgat acgtgcggca ccggagggga tggagcaggc
     1561 acgttcaata tctcgacagc cgcagcgttc gtcgccgccg gcgccggcgc ccgcgtggcc
     1621 aagcatggca atcgctccat ttcgagctgc tgcggcagcg cggacgtgtt ggaggaactg
     1681 ggcgcccgca ttattctttc acccgccggc atggcttcct gtgtgcggga ggtgggaatc
     1741 gggtttctct tcgcccccgc cctgcacccg gcgatggctc atgtgcagcc ggtccgcgcg
     1801 gcgctgaaaa tgcgcaccgt tttcaacctg ctgggacctc ttgcgaaccc tgccggagca
     1861 gaagtgcaaa tcgtcggcgt attcgagcga agactggtcg tgctgcttgc caaagccctt
     1921 gcccagatgg ggctgaggcg gggcctcgtg gtccacggca gcgacggcct ggatgaaatc
     1981 accgtcaccg gtcctaccga ggccgccgaa atcgtcgaag gcagagtcat tgagcgcacg
     2041 ctgctgccgg aggagtttgg gcttcaacgc cgtccggcgt atcaaatcgc cggcggcggg
     2101 cggcagagga atgcggccat tgtccgcgcg gtgctgagcg gtgaagaggg tcctcatcgc
     2161 gacatcgtgg tggcaaatgc cgcagcggcc ctggtgatgt ccggcatcgc tccggatttt
     2221 ccagcgggcg cagcggcggc cagagactcc atcgattccg gcaaggcggc cgagacgctg
     2281 ggccgcttca tccgtttcac gcaggcccaa tccggttgaa gcggcaggga tcagggcgag
     2341 atcttttcga gccgccatcc accggtttcc acgcacaaca ggctggcccc aaaagcggcg
     2401 agacaaacac acccaaactc caggaagacc agcgccagac tgtaatgtcc gatggtatag
     2461 ccgatcagca ggggcccaat gatggatgcc aggcggagcc aggcgctggc cacgctcgag
     2521 ccgagcgccc ggatgcgcgt gggataaatc tccggcgtat agaggaacag cagcatcgca
     2581 atggaattgg cgaagaatga tccggcgcag gtgtagctca agaccgttat cggagtggag
     2641 gctccggtga gccacagcgc cagaaaagcc agcccgccga gcaggaaaga tgccgcgaac
     2701 caagtccgcc gtcctgtccg gtcaactagc agggccaccg ccacgcaccc cacgagtccg
     2761 gcggcgctgg tgaacaggcc atagcgcaaa gaggtctgga tcggcgttct aaaaaccgtg
     2821 cggtacagag tcggcaccca ggtgctcaac ccgttcagca ccaggtaagc cgaaaaccac
     2881 agcacccatg caagcagcgt gcgccagcga tagattccac ggaacagatc tgcccactcc
     2941 gccttggctc tcatccgttc tccgccggca gcggcgttgt gttgcgccag ttgaccgttg
     3001 cgccacaatg cgccctgctc aatctgttgg acggcccagt ccgcctcccg cagacgcccc
     3061 tggctggcaa gccaccgggg agactccgga agcgcccgac gcaggccgaa aatgagcaat
     3121 gcgggaaggc cgccgatgag aaacatataa cgccagccga gccgggccac gattgatacg
     3181 cccagcagag cggctgccac tcgcccggcg gggaaaacaa gctcgtaggt gagtacaaac
     3241 ctgccccgag tcttcgcggc ggcgagctcg ttgatatagg ccgcggcgat gggaacttca
     3301 ccgcctaatc cgagaccttg cagcgtccgg aaaatcagca gagacgaata gctccaggag
     3361 accatgcaca gcaaactgca cacggaataa accagaatcg tacaggagag cgccgccagc
     3421 cggccgacgc gttccgcaag ccacccaaaa aagatggctc cgacgagttg gccggcgtat
     3481 ccggccgaga tgagcaaccc gacgcttctc ggagagatat gccacgggcc gatcagagca
     3541 ggcagtacat aggcaattgt gagcgcatcc acgccgtcga aaaaagtggc aatccccacc
     3601 agaacgcggg cttgcacgtg ccacatggaa accggcgcgc gctccagccg ggtcgcgatc
     3661 tgttcagccg tgagagtgct ggtcaaccgg aagcccctcg gtgaaaaatg ctagccgggg
     3721 tcgctggcca tatcggtatt tggcccactg tttctggcga agccgcagga tccccgaagc
     3781 acgccggaaa tgtgtatcag gttcccggcc gcaccaccgg actttgcttg tgccgggatg
     3841 catccagcgg cgacagcacg tgtcctgttc gatagggttg agtcccggct cttgcgcacc
     3901 ggatgaaagc aaatcataac ccgtgagggt gattggcaca agggccatag gccacgtgcg
     3961 atagaagtgc aggccaggcg tgcgataggc cacggtccca acgagctgtc cagacattgc
     4021 gggctgcaaa aaactctgcg cccccaagtc tcagaaagtg ctacagtgtg gcacggtggg
     4081 ccaagcctcc cctcgtttat caggcagcgg gggaaaggca cgcccgcggc agaaaccaac
     4141 tgcaacatcc gctaggaggc gacctcatga cccgtcgcaa tctgctttcc acggcggctc
     4201 tggctgctcc ggccgccgtt gacgctctcg ccaatgttcc gcagagcgac cgtccccgaa
     4261 gccagggaaa gccgtcgaag atcacgcgca tagaagcgac gcttttgcaa aagccgctca
     4321 aggagcgctt ctggatgtcg atgaacccga tcggtgggta caacccaaga gcctcgcgct
     4381 tgatactaaa aatgcatacc gacgccggcg tcaccggtta cggcgaaggg ggcggcgggg
     4441 gagccgagat gctgcgcaaa ggcttcgccg agctggtgat cggcgaagat cccttcatgg
     4501 tcggcaagat ctgggaaaaa atgttcggcg tgacatcgac ccgcgagatg gccgtcaaag
     4561 ggtggtcgcg cggcggcgtc attggcgtca tggcggctat cgacgcggcg atttacgacg
     4621 tcatggcgaa atccaccggg ctgcctattt acaagttcct aggcgcctat cgcaacacgg
     4681 tgccggtgta catcacggga ggctactatc gcgaaggcaa gggcatcccc gaactggtgc
     4741 aagaggtgcg cggctacgtc ggcgaaggct ataacgctct gaagctgaaa gtcgggggag
     4801 tgtcggcggg cttcagtccg gaagacgact acaatcgcgt gaaggctgtg cgcgacgcgg
     4861 ttggtcccaa cgtgaaaatc atgttggatg tcaacaacgg ctgggatctg cacacggcca
     4921 tccgcgcctc gaacaagctg gccgacctgg acatcacctg gctcgaagag ccgatccact
     4981 ggtatgacga tgtggagccc ctcaagcagc tcaagctcaa cacgcggatc ccgatcgcgt
     5041 cgggcgagca ggagttgacg cgctggagcg cgcggcggct catagagacc ggcgccatcg
     5101 actttatgca gttcgattgt cacgcgcatg ccggcatcac agaatggcgc aaaatcgccg
     5161 gcatggccag catgtgccac gttcagatgg cgccgcacca cgaaccgatt gttcacggcc
     5221 atctgctggc ctcgattccc aacggctaca tcctcgaagg cttcgccaat cctgaccgcg
     5281 atccgttttg gttcgagatt tatgataagc ggccgaagat tgagaaaagc atgctgtacc
     5341 tcgatgatac gccgggctgg ggcatcgaat tccggcaaaa agccctcgac cagtatggga
     5401 caaaggtaat ttagtttcga gaaccggcag gcgcggctgg cgcccccaac gcggttaggg
     5461 ttggcggcgg ccgatgagaa ttttggatct gccagggagg ctagccctgc gccggcctct
     5521 ctggccaact gatggcggaa ctgatctccc gctgcaaccg gagtccagtc gtccaaccgg
     5581 tccgaggatg tggagccaat cctcgcgcct gcgaggtccg gacagcgccg ccggacgata
     5641 cgcggttcca cgttcgccgg gctgggcgct cttttttccg cctgatgccc ggtttgcacg
     5701 ttttgccttc cgccatcgtc aagaaggtat aatgtcgagc gtgaaaaccc cgatttcccg
     5761 ccgccggctt ctcgcccgcg gcttctctct gatcgaactg ctgatcgtga tcgcaatcat
     5821 tctgatcatc gctgcgatcg cgatgccaaa actgaccaaa gcccgcatgt tcgcctacga
     5881 gatggccgca atccgcacgc tgcacacgat caacacagcg caggcgcaat acttttcgac
     5941 gtatggccgc tatgcgacca cattggctga gctcggtccg ccccccggag gcagcagtgt
     6001 agcaacggcg gccgccgccg acttgatccc cggcgacctg gccttaggaa ccaagtccgg
     6061 ctacaacttc acgatggtag gaacgcagca gggttatacg gcgaacgcga atccgcaagt
     6121 ctacaacacc acaggctctc gctcgtttta cacggaccaa tcgacggtca tccgggaaca
     6181 cctgggcaac gaaccagctt cggcggccga ccccgaaatc aagtaggaga aaacctagca
     6241 aaacgccgcg gacggtcggc cgctgtgcac gaagccacag gcgtcggcgg agtcacacac
     6301 aatgcttagc tcaaagggga aatccgccga gcgcccgcaa gctgatcact ccgagcggag
     6361 cgaggaatct gctttgcggt tcgccgagga gaaacagcag attgctcgct gcgccggcga
     6421 tagcggctat tggaagttga cgtttttgcc gtcagaatcg aagtagatgg cgtagcccac
     6481 gtcgatcccg gtagcctgaa agcgccccga ctgataatcc gagctgtatc cgatcgacaa
     6541 aaacccggcg ggcactcccg aaagagtgga gctcgctggc aaagccgaaa gtacgacgga
     6601 aggcaccgaa aactgaccgg cgctcgcccg ctcggtgcag atgaacgctc ctgatacctg
     6661 cgggttcgtg ttggccgagc cgccggttat gatcacaaac ccattgaggt ttgtcgggct
     6721 ccaggtaacc aacaaatcct gcgagcggag tacgtttgtg acggaatcct tgttcgtcca
     6781 ggttagcggg tccggcagcg tcagcgtcgc ccgaaacgcc cccacatcct tgccgccggc
     6841 gccattatcg atcgtgtaag ttcccttagt caggtacagc ggcgtaaatc ccggcagccc
     6901 gacggttcca ccccccagct gcgcactata ggccacgaca ccacccgtag aggtcttggg
     6961 aatctgcttg gaaccattcg gcccggttac tgtcagcgaa gctcccgcat cgagataggt
     7021 gaaggtcacg gggttgggaa tattggtgct attgccccga tactgataga tatagcaact
     7081 gccgtaaggg gcgtagcccg aggcgccggt ggaagtgatc aacttgctga aatcataacg
     7141 gacaaaagct ccgctcccgg cgtcgatggt agtaacgagg tttccgaccg gcggcggagc
     7201 cgttatgctt attgaagatc gggtaagcaa gacggacccg gcggagatgc tgccgccccc
     7261 attggcgatt ttttgcagat cgtcggcggt gaaaccgtta gaatcggagc acagcttccc
     7321 gctgctcgag attgaaatag tgaccgagtt gctgacgacg ccgttgaccc gcaacaaaac
     7381 cggcacgtag cacccggtga ttccctgagg cacctcaaac gcaacttgat ccagaccgga
     7441 acagcagccg gatcgcccga aaataacgat cttggccggc tttccgccga cgaaaacctc
     7501 aacggttacg ccgctgggag ctacgccggg cagcggccct gcggcttcgt caccgctcac
     7561 cggacccaaa ccggtggccc acaaaatcat aacctggccc ggcgccgctg atgcggtgat
     7621 caaattcgtg aaaaaattga catcggttat aactcccggg ccgctgcctg cctggttgac
     7681 cgcgaaaatt ccgggagcgc ttctcacgac gtgaattggc acggcagcac tggtctggcc
     7741 gttgtatgtc accgtaagcg tgccgtcacc cacgggggta ccggagggaa ggatggcccc
     7801 gacctggccg tcgaccgtgt acagcatgat cgcgttgacg gtcgtaccgg ccactgtaac
     7861 tttgatggaa gtgccggcca acgtggtcga gagcgggaac gaattggcct gcgctagagt
     7921 agagggcccc atccctttgc caaacacggc aaagatggat ccctccgcga ttccggaatt
     7981 ggggaaaccg gacgggagat aactggcgtt gctcaccacg ccatcggcat tgatgacggg
     8041 ctgcgcttgc aaagcggcag ccgccacgag cagaatcgcg aaaattttcc ccgttttcat
     8101 gactcctcca ctttcaccgg ccaacgcgat ccaagatcgc tccggctcct cctcgctgct
     8161 atgtatcaaa cgatacaagg aactacggta tcacgaaaca agggcaatcg agcttccctg
     8221 ccttctaaaa acagcagaag atttcgccgc cctccggccg aaggtcatat gccacgggag
     8281 agagcggctg tattcggcgg aatctcgagg ctggcgccga ttgggaggac cgttggaact
     8341 ggcggcaaca attcagacga actatcgcgc aggcgccggg acgaactgtt tgccggctga
     8401 caccggttcg atgcggccca tcaggagatg cacacagaac caagctaagg ggtgcaggat
     8461 tcccatcaat atgaacacgg gcacgtaccc gaagtaggta atgacaactc cggggaggat
     8521 cgcggaaaac agaaatccgc ccagtccgcc cgccgaagcg ccgatgccgg tgacagaggc
     8581 caccgcaccg gaggggaagc agtcggagac ggtggtgtac agattggccg accatccctg
     8641 gtgggcggat gtcgccacgc tgatcagcgc gatggccccc accgggtttc ccacgaatac
     8701 cgccatcacc gccagcggca tgcaggccgc cgccagcagc atggctccct tccgggcgcg
     8761 gccgacaggc cagccgcgcg caatcagaaa gcgcgagatc cagcccccgc ctacgcttcc
     8821 gaaatcggcc gcgagataga tcgccggaag cgcccagccg atctgcatga gactgaaatg
     8881 gcgtgcatcg taaaagtacg gcggcagcca agtcaggtag aaccaccaaa ccgggtctgt
     8941 caaaaacttg ccggcagcga agccccaggt ttcccgatgg cggagcgcgg ccacccacgg
     9001 tacagccgga gccgcctcct cccggcccga ctgaatatat tccagctcgg cgcggtcgat
     9061 cttccgatgt ttctccgggc ggtcgtagta tcgccaccac aggggaaccc agaacaggcc
     9121 gaggcccgcc gtcatcagaa aagcggcgcg ccacccgcag accgctgtga cggcgacaat
     9181 caccggaggc ccgacggtag cggccacatt cgtgccggcg ttgaaaattc cggtggcgaa
     9241 ggcgcggtcc tttttcggaa accactcggc cacggacttg atcgccgagg gaaagttgcc
     9301 cgattcgccc aatcccagca taaagcgcca tagtcccagc gcaattccgg atccggccac
     9361 cgagtgtaag gccgccgcca tcgaccacca gagtatcgat acgaggtagc cgattttcgt
     9421 gcccgcccaa tctatgaact tgcccatgaa caggaagccg atggtgtacg caagctgaaa
     9481 agcgccgttg atgtagccat actcgagatc gttcaggcgc aggtccttgc ggatggccgg
     9541 gatcagcacg gcgaagacga tccggtccag atagttgacg gtcgtagcga ggaacagcag
     9601 actcagaatg acccagcgga ctcgtgtggc gcgcatgcaa ggcggattat agcgaacctg
     9661 gcatgctaaa atcgtcgttg cagcgggatt ccacaacgga gaagacagag aaaaggcgct
     9721 gcgtcaccgc gcgcggcctt gattttcagc gtattctcca cggaggcctc ttgaaccgca
     9781 gaccttccgt tgtaacagac gtaggcggcg taaaagtggg cggcacgcac gcgatcgtgg
     9841 tgcagtcgat gaccaacacc gacacggcgg atgtgacggc caccgtaaat caagtgatgg
     9901 cgctggcggc cgccggatcc gaactggtcc gggtaaccgt gaaccacgac aaggccgccg
     9961 aggccgttcc ccgcatcgtg gacacactag aaaaattcgg agtcagggtc ccgattatcg
    10021 gcgactttca ttataacggc catattctgc tgaagaaata tcccgagtgc gcgcggcggc
    10081 tggccaagta ccgtatcaat ccgggcaacg tgaatatcgg ccacaagcac gacaacaact
    10141 tccggacgat gatcgacgtc gccatcgagc accgcaagcc ggtgcgcatc ggcgtgaact
    10201 ggggatcgct cgatcaggtc ctgcttacca ggctcatgga cgaaaactca cgcctgcccg
    10261 agccgcgcga tgcggccgag gttaccactg aggcgatcgt gcagagcgcc ttgaactcgg
    10321 cccgcgccgc cgaggagtac ggcttgccgc acgactcgat catcctgagc gccaaggtct
    10381 cgcgcgtgcc cgatctggtt cgagtgtacc gggaactggc ggcccgctgc gactatcccc
    10441 tgcatctggg gctgaccgag gcggggatgg gcgccaaggg gatcgtcgcc accgcagcgg
    10501 cgctggccat attgctccac gaaggcattg gcgacactat ccgggcttcg ctaacgccca
    10561 tgccggacgg cgaccgtacc gaggaagtac gaatttcgca gcagattttg cagtcgctcg
    10621 agatccgcag tttcacgccc caggtcacgg cctgtcccgg gtgcggccgc acaaccagca
    10681 cgttttttca agagatggcc ggccagattc agaaatatct gcgcgagcag atgccggtct
    10741 ggcgcgagcg ctatccgggc gtcgaggaat tgaaggtagc agtcatgggc tgcgtggtga
    10801 acgggccggg cgagtcgaaa cacgccgaca tcgggatctc gctgcccggc acattcgagg
    10861 aaccgaaggc tccggtttat gtggacggac gcctcgtggt aaccctcaag ggggccgaca
    10921 tcgtccccga gtttatacag atcctgaatc actacgtcga agcccgctac acggctgtgc
    10981 ccgcctcgcg atgacaccta gccgccacag ggcgccagcg aaatcaccaa aaaatcgtcg
    11041 ttgctgacca atacttggca ggcagccgcg cgtatgcgaa tcggccgatg ccggttgcag
    11101 actacgatcg tcttagcggg atcgaaacgc aaagaaggtc ccggatgagc agctcggtag
    11161 tcaataggct cttccagaag atgcgccgct gcgagatgca atgcccggat ctccgagtcg
    11221 cctaacgggt ccatccacca gggctgcaag ggcttttggg gtacgtaggc gggcaggatc
    11281 tgatcgagca gaggatcatt ggtcagaaaa ccttcatgcc gcaagccggg catactcaaa
    11341 actgcgagca cttggtccac cgaccgatcg gttgtgtgca actccctctc ccgggctacc
    11401 ggttgagcga gccggaaggc gcactgggcg aacccgagag ccactgtgcc cgcgacgatc
    11461 aaaccggcgc gccgccagag cacttttccc agaccggact gactccagcg ccagtagccg
    11521 atatcgcaca aaaagatggg gaagaagggc cccatgaaga acaacaggta gtggaacggc
    11581 tgcatcgttc tgccgctgat gacctgctca ttggtgactg cgaagcaggc gatccagaac
    11641 accgcccaaa accaaccgtt gcgccagccg cggcggcccg ctccaagggc cagcgccgcg
    11701 aataatgagc agaacaggag cgcgagccag tccggttgcc gggtgattgt ccccccaagc
    11761 ctcacaaaga agtcttgaac ggccgccggg cgggccgcca gcggaagcca aacggggatc
    11821 gaggcggctg cggccagccc ggctggcacg gcgatgaacg cagccagaag ccgcctgccg
    11881 gcattttcgg ctccggcaaa tggcaagccg gagccgagca ggatcagcca cacaatcagc
    11941 atcgccagcg ccagggtcca ggaatatacg taggtgtaaa agttcaatag cagagcaaac
    12001 cccaagaaag cggccgacac gatggagcgc cggcggtcca agcgcagcat ggcgatcagg
    12061 aacagcgcga agaagcaaca agaaatctgc gggtaagctg ttctggcgaa gatcggcccg
    12121 tagtgagggt ctccccagta aagatacaga aggccgatca gatcgaagaa gctgaaccac
    12181 accgcgaggc tcaccgcaaa caaccaaaga agcagacacg gaaataactg ccggcacagc
    12241 aggagcaggc agaaggccgt gagagccggg aacaaaaaat cggccgccac aaacgccttg
    12301 ggaacgctgc ggccgaaggg cgctgagagc gcggccagca aaatcgggcc cagccggtca
    12361 cggcaccaga ttgattgcgc gcgcgcctcg ccctttacgt atggctcgaa cgacccgagc
    12421 gtagctccca ggaagtcgcc gcgcaagacc tcctgtgcgt atcgggcata ggcggccgtc
    12481 tcatcccagg cgacatgatc gagggcgtaa aaatatttcg gcgagccgac tccgcgcacc
    12541 atccactggt cataaaccag gggcgtgtaa gaccggccgc aataatacat accagccagg
    12601 tgaccgaacg agaaggagat tccaaccaga agcgaaaaca gcgccagaat ccggttcgcc
    12661 ttgccgctga tcgggtcaat catggcttcc gggaaaatgg tagacaagaa tcgagtggcc
    12721 cacgcgcgcc gccggtgtgt ggccggcaag ccaggccaag tcggacggac tccggccgat
    12781 ccctcccata tgattcttgc tgacggccac ccagccggag gggcgcattc ctgccggcag
    12841 agatcgcacg tcggggattt ccaaaaaatc ggggccgggg cggccgaagt agctgaggta
    12901 aagggtcgaa atgccgtgct gccgcgcgaa ttgccgcagc cgctccaggt cctggcccca
    12961 atccaaattg ctgtccagca gaaagtgctc ctctcgtccc cgggcgattt ggttgaaata
    13021 ggccaggtaa tcggggtgtg cggcgattga ctccacgccg tgccaggcca ataggctcca
    13081 tgcgaggccg gccagtatgt gtccgcccgg ccgggagctc gtactgcaat ctgcgaacag
    13141 gctggcggca agcagagcca gaaaggggaa gatcggcagc agatgccgga tgccgacatt
    13201 gatgcggctc atcgcaccca ccgccagcat cagggcaacc gcgatcgccg gataagcggc
    13261 ggtggacagc gagtccgtcc agctctccac aacgcacagc gctagcgcga tggcagagag
    13321 cagcagaagg ggcagcgtcg tcttcaccgc taaagcgaca aggaaataga acgggaaact
    13381 cgaagaagaa actcgcccca gcagatacgc tttgtgcgcc cgagcattca gagcgaacac
    13441 ggaaagcaga ccgcgtatca gggttggcgc aggaaggaag atccttccgt gcagcgtgcc
    13501 gacttcgatg gcgctttcgg catattggcc caga
//
LOCUS       DASREK010000166        10807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_82702, whole genome shotgun sequence.
ACCESSION   DASREK010000166 DASREK010000000
VERSION     DASREK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82702"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VFA54_08575"
     CDS             complement(<1..927)
                     /locus_tag="VFA54_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZS50900.1"
                     /translation="MKDIARDLGVSLMTVSKALRNHSDISSETRSRVLKRARDLHYEP
                     NWIARSLVTRRTYMVGLVIPDLMHSFFAEVAKGVARKVEPLGYQIVISNSEENVEAEQ
                     RQIALLLSRNVDGLIVASTQPSGRTRLFKGLRSRKIPYVLIDRMPAGLEAHYVGVKDD
                     EIGALATEHLIEQGCRRIAHIRGPALSNGTGRLRGYRRALAKHALKPPPEYVVSAQSD
                     SSGYEAMRQLLRVSPRPDGVFCYNDPTAAGAIQAVLDAGLDVPHDVAIVGAGNVHYSD
                     LLRVPLSTVDQSSSLIGETAAELLAECMEAKTH"
     gene            1285..4728
                     /locus_tag="VFA54_08580"
     CDS             1285..4728
                     /locus_tag="VFA54_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50901.1"
                     /translation="MSFRPQYLLSRFAFWGVCSLGLCLANGVAFGQTATGQVNGTVTD
                     QSKAAVPSATAILVNRATGIETRASSNASGFFTFINVSPGVYTLRIEASGFRAAQIPE
                     FTVGVNQTVTQDVGLLLGQVTEVVEVAAQAESLQQSSAELGTVIAQQAVQELPLNGRN
                     FTQLLTLTPGATPVSTSQGAQIGVNDGSTVSIPRSGFSNPSIHGQFNRSSVYFLDGII
                     NTDFRTTTYTILPNIDLIQEFKVQSHNDKAEYGGVAGGVINVVSASGTNQFHGSAYEF
                     VRNNDFDARDSFFDATRTSPAPYRQNQFGATLGGPVIKNKTFFSAGYEGWRYSSPTGS
                     RYRVPTDPEISGDFSNSIIAHNIYDPLTTRPNPSNPNQLIRDQFPGNMIPSSRISPMT
                     QGFIQTYFDRPNLSDPVFNVILNQAQTNDNNSWQVKADHQFNEKNSLFFRYSGMNVHQ
                     NTPTTLKQNNYLQMTAKNFGGGVLHTFTPTLILDVRGGFASRPFLFGTDSSAGLAPQA
                     KLGFTGTDQFGGVTLSLLSPWGSAGIGPPQPRGNPVWSFSPNLSWLRGNHNIKTGFQI
                     ISVNRLQIAPGINYQFDDATTSDPQQPGKTGASLASALLGMPRTFSGNSAQKNRIDFD
                     IPIWSAYVQDEWRLKPAVTLTLGLRFDYVGRPTLNAGMNNGPNMLTGNWEIGGGKLPP
                     PCNQGGGAPCIPGNGLQDVPFSEHIVVAKDPLRWRQPQWNEFGPRISVAWRVTNRIVV
                     RAGYSILYDALPAQSQTYQNTLNQWPFSGGFSDTANAIGAPPTFIQDLQGSFPLPLPA
                     ASPWGISTWFSAPDRQDAQSHQWNVEIQRQMTDSLLLSAAYVGSYNRHIDVNGLANTA
                     VTPGPGSPDQVNQRRPYPWMGSFPYDNSIGNSNYNAFEFKASRRFARGFQALVSYTWS
                     KSIDTASSGWYSAENGPGGSSSFQNYYDLNGSRSVSSYDIPHFLSISSNWELPVGRGK
                     ALLNKGIAAWILGDWQANMIAQFRSGQPFTLSVPGDVANIGNSRPQSNYARPNLIGDP
                     YLSNPTASQFFNTAAFQIPIFSYGNFGRNVLRGAHVSNVDFSLFKTLPISQEGRRKLE
                     FRAEAFNVLNIMNWAPPGSNGALGSGSAPGATVGQTGFGRVTALAAFPRTIQLGLRLL
                     F"
     gene            4747..6000
                     /locus_tag="VFA54_08585"
     CDS             4747..6000
                     /locus_tag="VFA54_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS50902.1"
                     /translation="MSRGFLSFLLALVIVFAPLPSAVAQALDRAPADEKSQSGRNAGA
                     SGSELLQQGRDLLAKGSAAEAVPLLRQAIEKNPADADAHLLLGSALALIPRRSEAVAE
                     LALAIELRPDHAPAYNVLGMTLARFAEFDSAQKAFEKAIAIDPKLAGAHLNLALVFAQ
                     KGEIARASRAVARAIELYGDTVSAAYPHYVAGRLYNQQNLPGRALQEFEAAIRLNPKF
                     SEAFLELGSAKRKLSDEAGCIDALRKAVELAPENAEARYRLGVEYLNRRQAAKAVEHL
                     GAAYRLKPGDRGILYNYSRALRLEGQEEQAKIIFRKLSETVSATTKASQHLAEAARLT
                     NEGVALEKQGDFRAAVEKYRAALELDPLEASVRRNLGLALCRAGRWSEGIAELQEAQR
                     IDPNDAETTRALYIAREQEAAAQGR"
     gene            6022..7020
                     /locus_tag="VFA54_08590"
     CDS             6022..7020
                     /locus_tag="VFA54_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZS50903.1"
                     /translation="MSKTKILPVMLTLLLSAGGRADAQNRAAWMREARWGVMTHYLAD
                     WQARVHHLSMSVSEWNKLIDGFDAEGIARQLQSVGAGYYQISIGQNSGYYLSPNAVYD
                     RLAGIQPSKCSRRDLVADLSKALSKRRIKLMVYLPSGAPNQDKAADAALEWRNGPYPN
                     KEFQRKWEQVIREWSLRWGKKVSGWWFDGCYFPNSMYRSPDPPNFASFAAAARAGNPD
                     SALAFNPGVVYRMLSITPHEDFVAGEIDKPDLVTIRRAVDGKVDGAQIHMLSYLGQTW
                     GMGSPRFTADQVIAYTRKILEAGGAVTWDVPVELNGTISQPFVDQLTALKKALSSQ"
     gene            complement(7076..7759)
                     /locus_tag="VFA54_08595"
     CDS             complement(7076..7759)
                     /locus_tag="VFA54_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS50904.1"
                     /translation="METEVSLVGLSRERERIEKALENRESLLLLGPRGAGKSRLLHEV
                     VAQRPEAVYLPCPPEPHELLVSLARRLNVDFRLSSVRLRGLLWRDLEASPRILLLDGV
                     HHAGRALRSFLERLYYAPGSAILAAARGYGELGALEGLFWDPRQVVRFKPLSPDEARL
                     LFEAAARRFGLGALELDDFREYVLECARGNPGEIVEMCRLACNPRYLTGARIKFHILR
                     IDALMQLGG"
     gene            complement(7764..8186)
                     /locus_tag="VFA54_08600"
     CDS             complement(7764..8186)
                     /locus_tag="VFA54_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZS50905.1"
                     /translation="MFDSILVAHDGSDGAQKAFDAALELALRYQAKLHMISVEEDLPR
                     YAETVAEVSEASEEASRYFGQLAEHAKRRAAMRSVELECTIVAGHEVKAIVDFVRARG
                     FRLLVVGFTGHSRIHDSLWGGTSQNLARLAPCSVLVVK"
     gene            complement(8179..10062)
                     /locus_tag="VFA54_08605"
     CDS             complement(8179..10062)
                     /locus_tag="VFA54_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HZS50906.1"
                     /translation="MTAAQSERETLRSLLADLRRLSERFHADSISRSAGELCSRLERS
                     RFHLVVAGQFKRGKTTLLNALLGRELLPAAVLPLTSAVTMLHYGPEIQAEIVLESGAR
                     LPIRTEQLAEFVTERGNPKNFRRVSWAEIACPNDFLREGLVLIDTPGIGSVYKHNTQV
                     TCDFLPRIDGAIFVTSPEPPLGEVELQFLKELKERSGRLFLVLNKADQMEPRELAEVL
                     AFTREAAQEDRIFAVSARLALEAKLRADSALLESSGLPAFEKELQRFLHREKNQVLLA
                     SVLRGARQQMADLRLELSLEIQAARLPVQELDAKASELQRELQHARQQQEESSLLLHG
                     AIDRLRAWVDAEAARFAAAEAGPLLAAVEAHWRQARGLPRNQFAKSMDGFVQRAIWER
                     CDAWRGALEPAAAAQLREITGRFEERTNKLIRSVCEIAGRLFGISVPELAGGGELIGM
                     EGSGYYTDPLLDWGLGSAPLLLPPPLFRQYLLRRLRRAAPAELERNSNRVAIDLKRRL
                     EKSVAFFQEAMNRRLEETVQALERALASAVERRQASAAEAGAGLEELTSGICELERLA
                     ERAGRLDAAATIAAGSGSESYQSSRQPRAAQRSCGERSRVERRLSPCARVDPGAERGA
                     SNV"
     gene            complement(10059..>10807)
                     /locus_tag="VFA54_08610"
     CDS             complement(10059..>10807)
                     /locus_tag="VFA54_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZS50907.1"
                     /translation="ASCFINDLGTVIALGLIFAPFTGRTVIFLAASFVLFVVLPFATP
                     WFFERYGGRVSELEAKFILFFLFLTGGLAVWAGSEAVLPAYMIGMVLAGAVGRDHILV
                     RRLRTLTFGLLTPFYFIRAGSFVSVPALVAAPLAFVALFFAKMLSKIAGLAPAIRRFR
                     YATREVLYYSLMMSTGLTFGTISALFGLNHGVITQAQYSYLVAAVIGSAVIPTMVANA
                     YFLPKHLLPGREPVEALVAEAPSAAVEESG"
BASE COUNT         2200 a         3383 c         3180 g         2044 t
ORIGIN      
        1 atgcgttttg gcctccatgc attcggcaag cagttcggcc gccgtctcgc ctattagcga
       61 actgctctga tccacggtgg atagcggaac gcggagaagg tcggagtaat ggacattgcc
      121 ggcgccgact atcgccacgt catggggcac atccagcccg gcatcgagga ctgcctgaat
      181 cgccccggct gcggtgggat cgttgtagca aaacactccg tccgggcgcg ggcttacccg
      241 gagcaactgc cgcatcgcct catagccgct gctgtcgctt tgcgcgctca ccacatattc
      301 gggaggcggc ttcaacgcat gttttgccag ggcccgccga tagccccgca gacgccctgt
      361 gccgtttgaa agcgccggac ctcgaatgtg cgcaatccgg cggcagcctt gctcgataag
      421 atgttccgtc gccaaggcgc cgatttcatc gtccttgacg cccacgtaat gagcctccaa
      481 gccggccggc atccggtcaa tcaggacgta aggaattttg cgggaccgca gccccttgaa
      541 cagccgcgtg cgcccgctgg gctgggtaga ggcgacgatc agtccatcga cattccggct
      601 cagcagcaac gcaatctgcc tctgctccgc ctcgacattc tcctcggaat tggaaatcac
      661 gatttggtag ccgagcggct cgacctttct cgccactccc ttggccactt cagcgaagaa
      721 agaatgcatc aggtcaggta tcaccagccc taccatgtac gtgcgccggg tgaccaggct
      781 gcgcgcgatc caattcggct cgtagtgcag atcgcgggct cgtttgagca cccggctgcg
      841 tgtctcggag ctgatgtcgc tgtgatttct cagggccttc gagaccgtca tcagagagac
      901 gcccaggtcc ctggctatgt ccttcatccg agccgccaag cttcctcctc gccgcccgct
      961 ttccggccgc ccgacatcat gtttatcacg aacggcggcc gagatgcatg tccgcttccc
     1021 ctgcccgagc tccctgcacg ccgcctcgtc ggcccgtcca cttggaacac gttctctgtc
     1081 ggaaatttac tttaacgcta cagggagcgc tctagatcta gaatttttgc aaaacaatgc
     1141 ttgacaggat ttttcaggca gtgatagaat gcccgcacac tcggcaggcg cacaagcagc
     1201 gagcggatac tcgccggcga acgagcggga aaggcgcccg tacaatcgca tccgcggttc
     1261 gcggcagaga agggggacca gaccatgtcg ttccggccgc agtacttact gtcgcggttc
     1321 gccttttggg gagtttgcag cctgggtctt tgccttgcaa acggcgtcgc cttcggccag
     1381 acggccaccg ggcaggtcaa cggcacggtg acggatcaga gcaaagcggc cgtgccatcg
     1441 gcgacggcga ttctggtgaa ccgggccacg ggcatcgaga ccagagccag cagcaatgcc
     1501 agcggctttt ttacgttcat caacgtgagc ccgggcgttt acactctgcg gatcgaggct
     1561 tccggtttcc gggcggcgca gattccggag tttaccgttg gggtcaatca gaccgtcact
     1621 caggacgtgg gtctcttgct cgggcaggtg accgaagtcg tagaggtagc cgcccaagcc
     1681 gagtcgttgc agcagtcgtc ggccgaactg ggcacggtca tcgcgcagca ggccgtgcag
     1741 gaattgccgc tcaacggcag gaacttcacc caattgctga cgctgactcc gggcgccacg
     1801 ccggtcagca ccagccaggg agcgcaaatc ggggtcaacg acggatcgac cgtatccatt
     1861 ccccgttccg gcttttcgaa tccctcgatt cacggccagt ttaaccgctc atcggtctac
     1921 ttcctcgacg gcatcatcaa caccgacttc cgcaccacga catacacgat cctgcccaac
     1981 atcgatttaa ttcaagagtt caaggtgcag tctcacaatg acaaagccga gtatggcggg
     2041 gtggcgggag gcgtgatcaa tgtggtctcc gcctccggca cgaaccagtt tcacggctcg
     2101 gcctatgagt tcgtgcgaaa caacgacttc gacgcgcgcg attctttttt tgacgcgacg
     2161 cggacttccc ccgcccctta ccggcaaaat cagtttggcg caacgctggg cggtcctgtc
     2221 atcaagaaca aaacgttttt ttcagccgga tacgaggggt ggcgttactc gagtcccacg
     2281 ggctcccgct atcgcgtgcc gaccgatccg gagatttcgg gcgatttttc caattcgatt
     2341 atcgcccaca acatctatga tcccctcacc accaggccca acccgagcaa tccgaatcag
     2401 ctaattcgcg atcagtttcc cggaaacatg attccgtcca gccggatctc gccaatgacg
     2461 cagggtttta tccagaccta cttcgaccgc ccgaatctgt cagatccggt gttcaatgtg
     2521 attctcaatc aggcgcagac gaatgacaac aacagttggc aggtgaaagc agatcatcag
     2581 ttcaatgaaa agaacagcct gttttttcgc tacagcggga tgaacgtgca ccagaacacg
     2641 cccacgacgc taaagcagaa taactatctg caaatgaccg cgaaaaactt cggcggcggc
     2701 gtgctgcaca catttacgcc aacgctgatc ctggatgtaa ggggaggatt tgcttccaga
     2761 ccgttcctgt tcggaaccga ttccagcgca ggtcttgcgc cgcaggccaa gctgggcttt
     2821 acgggaaccg atcagttcgg cggcgtgacg ctcagtttgc tgtccccctg gggaagcgct
     2881 ggaatcggcc cgccgcagcc gcggggcaat cctgtctggt cattttcccc aaatctgagc
     2941 tggcttcgcg gcaatcacaa cattaaaacc gggtttcaaa tcatcagcgt caaccggttg
     3001 cagattgccc ccggcatcaa ttatcagttt gacgacgcaa cgacctcgga cccgcagcag
     3061 cccggcaaga ccggggcctc tctggcttcc gccttgctgg gcatgccgcg gactttcagc
     3121 ggcaactcgg cgcagaagaa ccgtattgat ttcgatatcc ccatctggtc ggcgtatgtg
     3181 caggatgaat ggcggctcaa gcctgctgtg acgctgaccc tgggcctccg tttcgactat
     3241 gttggccggc ctacgctgaa cgccggaatg aacaatggtc ccaacatgct gacgggaaac
     3301 tgggagattg ggggcggcaa actgccgccg ccgtgcaacc agggcggcgg cgccccttgc
     3361 attccgggca acggcttgca ggacgtcccg ttcagcgaac acattgtggt cgcgaaagat
     3421 ccgctgcgct ggcgccagcc gcagtggaac gagttcgggc ctcgcatcag cgtggcctgg
     3481 cgggttacca atcggattgt ggtcagagcc ggctacagca tactctatga cgctctgccg
     3541 gcgcagagcc agacatacca gaacactctg aaccagtggc ccttcagcgg cgggttcagc
     3601 gacacggcca acgcgatagg cgcgccgccg actttcattc aggatttgca ggggagcttt
     3661 ccgctgccgc tgccggctgc gtctccgtgg ggcatctcca cctggttcag cgccccggac
     3721 cgccaggatg cgcaatcgca tcagtggaac gtcgaaatcc aaaggcaaat gactgacagt
     3781 ctcttgctgt cagcggccta cgtcggcagc tacaaccggc acatcgatgt aaatggcctt
     3841 gccaacacgg ctgttacgcc gggtcccgga tcccccgatc aggtcaatca gcgccggccg
     3901 tatccctgga tgggatcgtt cccctacgac aacagcattg gaaattccaa ctacaatgcc
     3961 ttcgagttta aagcttcccg ccgattcgcc cgcggatttc aagccttggt ctcctatacc
     4021 tggtccaagt ccatcgatac cgcaagcagc ggctggtatt ccgcggagaa cggcccgggc
     4081 ggcagctcga gctttcagaa ttactacgat ctgaacggga gccgcagcgt atcttcctac
     4141 gacattcccc acttcctttc catcagctcg aactgggagc tccctgtggg ccggggcaag
     4201 gcgctgctca ataaaggtat tgcggcctgg atactggggg actggcaagc caatatgatt
     4261 gcgcagtttc gctctggcca gccgtttacc ctgagtgttc cgggtgatgt cgccaacatc
     4321 ggaaacagca ggccgcaatc gaattacgcg cgcccgaatc tgatcggcga cccatatctt
     4381 tccaatccga ccgccagcca attcttcaat accgccgcgt ttcagattcc cattttcagt
     4441 tatggaaact tcgggcggaa cgtgctgcgg ggcgcgcacg tctccaacgt ggatttctcg
     4501 ctcttcaaga cgctccccat ttctcaagag gggcggcgga aactggagtt tcgggccgag
     4561 gcgtttaatg tgctgaacat catgaactgg gcccctcccg gctcgaacgg agctctgggt
     4621 tcgggcagcg ctcccggcgc gacggtgggg cagacaggct ttggccgggt tacggcgctc
     4681 gccgcatttc cccgaaccat ccagctcggg ctaagactgt tgttctaaaa ggcggcgacc
     4741 aagtagatga gtcgaggctt tctgagcttt ctgctggcgc tcgtaatcgt gttcgctcct
     4801 ctaccctcag ccgtcgccca ggcgcttgat cgagcgccgg ccgatgagaa aagccaatcg
     4861 ggcagaaatg ccggcgcatc cggctctgag ctgctacagc aaggtcgaga tctgctggcc
     4921 aaagggtcgg ccgcggaggc cgttcctctt ctccggcagg cgattgagaa aaatccggca
     4981 gatgccgacg cccacctttt gctgggttca gcgctcgccc tgatcccgcg gcgcagcgag
     5041 gcggtggccg agctggcgct ggcaatcgag ctgcgccccg atcacgctcc ggcttacaac
     5101 gttttaggca tgacactggc gagatttgcg gaatttgact ccgcccagaa ggcgtttgag
     5161 aaggcgatag ctatcgatcc caagttggcc ggcgcccacc tgaatctcgc tttggtcttc
     5221 gcacagaaag gagaaatcgc cagggcgagc cgtgcagtgg cccgagcgat tgaattatac
     5281 ggtgacacgg tctcagccgc gtatccgcat tatgtggccg gaaggctcta caaccagcaa
     5341 aatctgcccg gcagggcgct ccaggagttt gaagcggcca tccggctgaa tccgaaattc
     5401 tctgaggcct ttctcgagtt gggatcggcg aaaagaaaat tatccgatga agcgggctgc
     5461 atcgacgctc tgcgcaaggc agtggaactg gcgcccgaga acgccgaagc ccgctatcgc
     5521 ctcggtgtcg agtacttgaa tcggcgtcag gcagcaaaag cggtggagca tctcggcgcg
     5581 gcttaccggc tgaagccggg cgaccgcgga attctttaca actatagccg cgccctgcgt
     5641 ctggagggcc aggaagagca ggcgaaaatc atctttcgga aactgtccga aacagtgagc
     5701 gcgacaacga aggcctcgca gcatctcgcg gaagccgcca gactcacaaa tgaaggcgtg
     5761 gcgcttgaaa aacagggcga ttttcgcgct gccgtcgaga aataccgcgc cgccctggaa
     5821 ttagacccgc tcgaagcgag tgtccgccgc aatctcggcc ttgcactgtg ccgcgcgggc
     5881 cggtggagcg agggcattgc ggagttgcag gaagcccagc gcatcgaccc gaacgacgcc
     5941 gaaacgacac gcgcacttta catcgcccgc gagcaggaag ccgctgctca aggacgttag
     6001 atcaagcgag gggaccggaa aatgtcgaag accaagatcc tgccggtgat gctgacgctg
     6061 ctgctctcgg ccggcggcag agctgacgcc cagaatcggg cggcttggat gagggaagcg
     6121 cgctggggcg tcatgacgca ttacctggcc gactggcaag cccgtgttca ccacctctcc
     6181 atgagcgtta gcgaatggaa caagctgatc gacggtttcg atgcggaggg aatcgccaga
     6241 caactgcaat cggtcggagc cggctattat cagatcagca tcggccagaa ctccggctat
     6301 tatctctctc cgaatgcggt ctatgatcgc ctagctggaa ttcagcctag taagtgctcc
     6361 cgccgcgacc tagtcgccga tttgtccaag gctttgagca aaagaagaat caagctcatg
     6421 gtctatctcc cctccggagc tcccaatcaa gataaggcgg ccgacgcagc gcttgagtgg
     6481 cgcaacgggc cctatccgaa caaggagttt caacgcaaat gggaacaggt gatccgcgag
     6541 tggtcgctgc gatggggaaa gaaagtttcg ggctggtggt ttgacggctg ttatttcccc
     6601 aactcgatgt accgctctcc cgatcctccg aatttcgcca gtttcgccgc agcggcccgc
     6661 gcgggcaacc ccgatagcgc gctggcattt aatcctggcg tcgtttaccg catgctttct
     6721 atcacgccgc acgaagactt tgtggctggt gagattgaca agccggactt ggtgaccatc
     6781 cgccgcgcgg tggatgggaa ggtggacggc gcccagatcc acatgctgag ttatctgggc
     6841 cagacctggg gtatgggatc tccgcgattc accgccgatc aggtgatcgc ttacactcgc
     6901 aagattctcg aggccggcgg cgcggtcacc tgggacgttc cggtcgagct caatggaacg
     6961 atctcacagc cctttgtgga tcaattgacg gcgctgaaaa aggcgctgag ctcccaatga
     7021 aagccgcggc ctttctccgc tgtggtggcg tgcccaggcc gcgcccgctt gggccttagc
     7081 cgcctagctg catcaatgca tcgatgcgca gaatgtggaa tttgatccgg gcgcctgtca
     7141 gataacgcgg gttgcaagcc agccggcaca tctcgacgat ttctccgggg ttaccgcgcg
     7201 cgcactctaa aacatactcg cggaagtcat cgagttcgag agctccgagt ccgaagcggc
     7261 gggccgccgc ctcgaaaagc aatctcgctt cgtcaggcga taagggtttg aagcgaacga
     7321 cctggcgcgg gtcccagaac agcccttcca gagcgcccaa ctcgccatag ccacgggcgg
     7381 cggcaaggat cgcgcttccg ggcgcatagt aaagcctctc gaggaaactt cgcagggcgc
     7441 ggcccgcgtg atgaactccg tcgagaagca gaattctcgg cgaagcctcg agatccctcc
     7501 acaaaagccc ccgcagccgc acgctggaga gacgaaaatc cacgttcagg cgccgggcca
     7561 ggctgaccag caactcgtgc ggttccggag ggcagggaag atagaccgct tcaggccgct
     7621 gcgcgacaac ctcgtggagc aagcggctct tgccggcccc gcgaggaccc agcagcagca
     7681 acgactcgcg gttttccaat gccttctcga tccgctcgcg ctcccgagag aggccgacga
     7741 gggaaacttc ggtctccata gagtcacttc acgaccagca cgctacaggg cgccaggcgg
     7801 gcgagatttt gggatgtccc gccccacaga gaatcgtgga tcctcgagtg tcccgtgaag
     7861 ccgacgacca gaaggcggaa ccccctggct cgaacgaaat ccacaatggc cttcacttcg
     7921 tgaccggcga cgatggtgca ttcgagttct acgctccgca tcgccgctcg ccgcttggcg
     7981 tgctccgcca attgtccgaa gtaacggctg gcttcttcgc tggcttccga aacctcggca
     8041 accgtttcgg cgtagcgcgg gaggtcctct tccacgctga tcatgtgcag ctttgcttga
     8101 tacctcagag ccagttcgag cgccgcatcg aaagccttct gggccccgtc ggacccatcg
     8161 tgtgcaacga gtattgagtc aaacattcga tgcccctctt tcagcgcccg ggtcaacccg
     8221 ggcgcaggga gaaagacgcc gttccactct cgatctctcg ccgcaagaac gctgcgcggc
     8281 cctcggttgc ctcgaacttt ggtacgattc gctcccgctt ccggccgcaa ttgtggccgc
     8341 agcgtccagc cggcccgccc gctcggccag gcgttccagt tcgcatatgc cagaggtcaa
     8401 ttcctccagc ccggcgccgg cctcggccgc actggcctgc cggcgttcaa cggcggaggc
     8461 cagagctctc tccagcgcct ggaccgtctc ctcgagccgc cggttcatcg cctcctgaaa
     8521 aaaggcgacg cttttctcca gccgcctttt gaggtcgatg gcgacacggt tcgagttgcg
     8581 ctccagttcc gccggagcag cgcggcgcag ccgccgcaga aggtactgcc ggaacagcgg
     8641 tggcggcagg agcagcggag cgctgcccaa accccaatcc agaagcgggt ctgtataata
     8701 cccggagcct tccattccga taagctctcc tccgccggcc aactcgggga cagatatccc
     8761 gaacaacctt ccggcgattt cgcataccga gcggatcagc ttattcgtcc gctcctcaaa
     8821 gcgtcctgtt atttcgcgca gttgtgcggc cgccgccggc tcgagagcgc cgcgccaggc
     8881 gtcgcatcgc tcccagatcg cgcgctggac gaatccgtcc atggatttgg cgaactgatt
     8941 ccggggaagc ccgcgagcct ggcgccaatg agcctccacg gctgccaaga gagggccggc
     9001 ttccgcggcg gcgaaccggg cggcctctgc atccacccat gcccgcagcc gatcgatggc
     9061 cccgtgcagc agcaggctgc tttcctcctg ttgctgcctg gcgtgctgga gttcccgctg
     9121 gagttcagaa gctttggcgt ccagttcctg caccggcaag cgcgcggcct ggatttcgag
     9181 cgacaattcc aggcgcagat cagccatttg ctgccgggcg ccgcgaagca ccgaagctag
     9241 aaggacctgg tttttctcgc ggtgcaaaaa acgctggagc tccttctcga atgccggcag
     9301 cccgcttgat tcgagcaagg ccgaatctgc gcgcagcttc gcttcgagcg ccagccgcgc
     9361 cgagaccgca aaaatccggt cctcttgcgc ggcttcgcgg gtgaaggcca acacttcggc
     9421 cagctctcgc ggttccattt ggtcggcttt gttcaacacc aggaacaggc gtcccgaacg
     9481 ctctttgagt tccttcagaa actgcaactc tacctcgccg agaggcggct cgggcgaagt
     9541 gacaaaaatc gcgccatcga tgcgcggcag aaagtcgcac gtgacctggg tattgtgctt
     9601 gtaaacgctt ccgattcccg gcgtatcgat cagaaccagg ccctcgcgca ggaaatcatt
     9661 cgggcacgct atctctgccc aactcacgcg cctgaaattt ttaggattgc cccgttcggt
     9721 gacgaactcc gcgagttgct cggtgcggat gggcaggcgg gcgcccgatt ccaggacgat
     9781 ctcagcctga atttccggcc catagtggag catcgttacc gccgaggtca gaggcagcac
     9841 cgcggccggc aaaagctcgc gtcccagaag cgcgttgagc agcgttgttt tgcctcgctt
     9901 gaactgccca gccaccacca ggtgaaaccg gctgcgctcc agccgtgaac agagttcacc
     9961 ggccgaccgg gaaatggaat cggcatggaa ccgctctgat aatctccgga ggtcggccag
    10021 cagagagcgg agcgtctcgc gttcgctttg ggcggcagtc atccggattc ctccacggcc
    10081 gccgaaggcg cctcagctac aagggcttct acgggttccc ggcccggcag caggtgcttc
    10141 ggcaggaagt acgcgttggc caccatggta ggtatgaccg cgctgccgat cacggccgcg
    10201 acgagatacg aatactgggc ttgggtgatg accccgtggt tgagcccgaa caatgccgaa
    10261 atcgtaccga aggtgagccc ggtggacatc atcagcgaat aataaaggac ttctcgggtc
    10321 gcgtagcgga atcggcgaat cgcgggcgcc aagccggcga tcttcgagag cattttggcg
    10381 aagaagagcg ccacaaaagc gaggggcgcc gccaccagag ccggaacaga cacaaacgac
    10441 ccggcgcgga taaagtagaa gggcgtcagc aagccgaaag taagcgttcg caaacggcgg
    10501 accaaaatat ggtcccgccc tacggcgccc gccagcacca tgccgatcat atacgcgggc
    10561 aaaaccgctt cgctgccggc ccatacggca agtcctcccg taagaaacag gaagaacaga
    10621 atgaacttgg cttccagctc ggagacccgc cctccatacc gctcgaagaa ccagggggtt
    10681 gcgaacggga gaacgacgaa taggacaaaa ctggccgcca ggaaaatcac cgtccgcccg
    10741 gtgaacggcg cgaagatcag cccgagtgcg atcaccgttc cgaggtcatt gatgaagcac
    10801 gatgcca
//
LOCUS       DASREK010000167         8847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_908458, whole genome shotgun sequence.
ACCESSION   DASREK010000167 DASREK010000000
VERSION     DASREK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908458"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFA54_08615"
     CDS             complement(<1..852)
                     /locus_tag="VFA54_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS50908.1"
                     /translation="MKTPWGTLPIADAHVHLLSRAFFEDLSTERTPEEMGRQLGWEIP
                     SKDAAELAGRWMQEMEKYDLKRLVLIGSFPGDENSVAGAVRTCPEKFVGYFMLNPTAE
                     DALQRAKHAIENVGLRGICLFPAMHKFSVSDDRLHPIYELASGRAIVFVHMGVLSVGV
                     RKKLGLPSLFDIRLSNPIALHPIALEFPRVNFVIPHFGAGYFREALMLGDLCPNVYLD
                     TSSTNSWIRYVPGLNGLTDVFRRTLDVFSVQRILFGTDSSFFPRGWNAQIFTEQVEAL
                     DRLGISAA"
     gene            1281..2453
                     /locus_tag="VFA54_08620"
     CDS             1281..2453
                     /locus_tag="VFA54_08620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZS50909.1"
                     /translation="MLSSVWFATLGDAERCRLEESDPLPASADIAIIGGGMIGLATAY
                     YLCEAGATGICVIERDTLVGEASGANAGGLWFGQQSQEMGPLSPLAQASSLLYEELAA
                     QPGWAFDFRRTGLLELLFDRESLAGADARAQAVRRAGFRAEVAGREDLGKLEPSVGDA
                     PEGAILYPDEGQLHPVKLGAAFARHLRNQNVRFSLRTEVSEVGDAVVRTSNGDLRADI
                     IIIASGAWTPLLTCTLGYRPPIKPMRGQLLATPPRTPFLRHTVIGRRFYYWQLTEGHV
                     AGGGTVEDVGFERGVSARDLTCIREEMNTLFPDLCASPTALSWSGFRPYCEDLRPIIG
                     RVPGCEQIYVAAGHFKKGIMLAPVTGKILADLITTGKTDLPIISLDPARFAAGGRA"
     gene            2622..3728
                     /gene="amrS"
                     /locus_tag="VFA54_08625"
     CDS             2622..3728
                     /gene="amrS"
                     /locus_tag="VFA54_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZS50910.1"
                     /translation="MSTLAEILAENARPGDLWEPIERNRIRCFACGHRCPIPEGQSGV
                     CKVRFNRGGQLFVPWGYVAGVQCDPIEKKPFFHVRPGALAFSFGMLGCDLHCSYCQNW
                     VTSQAIRDPNAVAPPLRTTPEQLVRLAIEEGGEVMVSTYNEPLITAEWAVSVFQAARA
                     AGLITGFVSNGNATPEVLEYIRPWVDLYKVDLKSFDDRHYHELGGRLQPILDSIRRIY
                     AMGFWLEIVTLVIPGFNDSNDELTRMAEFLAGISPDIPWHLTAFHRDYKMTTPEDTSP
                     DTLVRAAEIGRRAGLHYVYAGNLPGMVGNLENTYCAACGELLIARHGYRVQKYKVTPN
                     GLCPKCAAPVPGRWAEAFRRQITDHPFLPVLAGP"
     gene            complement(3758..4969)
                     /locus_tag="VFA54_08630"
     CDS             complement(3758..4969)
                     /locus_tag="VFA54_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS50911.1"
                     /translation="MRIVYFNYEWDLERSTGSATQIAETSAALERLGHKVVVIDRHRK
                     QAQRNAVKAPGHRHNWLWETANYWRSVRGVPLEINILRRERPDVVLTFHAHRFSSLIA
                     ARRLGIPIVFEVNASVPFETRRFHPQVHLLPGVSEWIEDRTLRAADRLMMVSNVLRDY
                     FLARGLPRERMAVIPNGADTNLFRPEAADQELRRRLPARLLIGFAGSFAPFHGLDLLQ
                     QAVLRISARHPEVHFVFAGGGPAAEALRKRWEANGCASRVTFLGLLPRKCVPGMLAAM
                     DILLAPYPAQNVFYFSPLKIFEYMACGRAVVAPRLGQVAEVIRHDYNGVLYDPARPDD
                     FVNQVLSLAQDPNRRMRLGAAARETIISQYTWDHHARLLAALLEQAVARPSTICWPNI
                     LGAGRRSCGAS"
     gene            complement(4976..7342)
                     /locus_tag="VFA54_08635"
     CDS             complement(4976..7342)
                     /locus_tag="VFA54_08635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS50912.1"
                     /translation="MKVLHVAYNTWPDVTGGAIRTRYTVEAQRRLGLEPVVLSAPFQR
                     AADPAQTSGVEYWNGIAYHRCYNGCDPSRFMAAHKSLWERAIKFAAFPAFVRRIRRLA
                     LDERVDIIHAHSLSFCGLAAAAAARGLGLPVVYEIRSLMEEGLDGAGVLVRGAYLLQD
                     LLACRLASHVVVICKALREEMIRRGISPGKITIAGNGVDIQVHRPGLPSNRVSGKEFT
                     IGYIGTLVPYEGLDLLIEAAALLTLKHPNLRLLIAGDGSARPALEEQARRLGLGLAVR
                     FTGGVPHEEVAPLYREIDLFILPRRSLRLTDLVTPLKPLEIMAYAKPLLASDCGGHRE
                     LVAPGVNGVLFPAGSAGALARRIEELMADPESLIRLGRSAREWVSEHRSWESQGEPLL
                     EAYKKLLARPSQASPKVLLVAPRPSALPTGGVETGVQMILRSALAKRYSIRLWRRQQY
                     TFFPNWRALRAARRFADFIGFSAAVAFLRPDIVHIKSSSGVNFAESAIYVLISRALRR
                     RALLQIHSGEFGHWYARGRTVSRWAIRCSLRAASEIIVLSTYWRDLLAQWVPDSRIHV
                     VPNGVRVPQIRPTSTSFRYGLRVLTLATLGSHKGHFDILSAAKLLRDEAVCFLLAGPD
                     QTSGFGEGQEVRRRAAELRVEANVRFLGPVSSQEKWNLLADADIFLLPSRAEGMPNSV
                     LEAMAAGLPVIATPVGALPEMLGYGCGGVLVSVGDFEGIARAVLDLGGDPGRREKMGN
                     WNRSRVRAMYSFEHVERLLDGIYAARSRSVPARESREALDAVARNWPA"
     gene            complement(7339..>8847)
                     /locus_tag="VFA54_08640"
     CDS             complement(7339..>8847)
                     /locus_tag="VFA54_08640"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HZS50913.1"
                     /translation="VGIRAVSWLWAVALLLDCDFLDETLLRRWLGCLGEHYQYLVSHL
                     SLYADPTNHLIGEAAALWMLSVAFSDLPGAKRQEQRAIEILSDQIERQVTCDGIGREQ
                     STGYQQFILDFYAQAAAIARNAGRELPAVIPQRMAAMRDFLAALTDEAGDPARIGDSD
                     DGRGIPFPELSKQREGFDKRDADVWIEGLEALQAPQQNHNTAISRAFREGGYSLWQAE
                     DAGDRIALLFDCGPLGLWPNASHGHADALSVQIKLRGRWILADPGTGVYEANRRIRDS
                     LRGTAAHNTVTVDYLDQTEALDTFKWLRPCPTRLLDIFSGAQYDFAAGMHEGYHRLRK
                     PVTHYRAVLFLRPPAHRSCWIVIDRLEGAGRHHCALRFHFPPGVEIRSEGWQVVSAVD
                     RQIGAGLRFCFSEPGWRLEKGIWSRRFGQWEPSPVISLERSADLPLCWFTFISPFTES
                     VSSGDATEIPQTWRQGDCIFCQRGEETVEWSIEPPAPRFRFQPAGGLACFER"
BASE COUNT         1848 a         2750 c         2605 g         1644 t
ORIGIN      
        1 agcggcgctg atccccagcc gatccagcgc ttccacctgc tccgtgaaaa tctgcgcatt
       61 ccagccgcgc gggaaaaacg acgaatctgt tccaaacaaa attctttgga cgctgaacac
      121 atccagcgtt cgccgaaata cgtccgtcag gccgttcaat cctggtacgt atcggatcca
      181 cgagttggtg cttgaggtat ccaaatagac attcgggcaa aggtccccca gcatcaacgc
      241 ctcgcgaaag tagccggctc caaaatgcgg aatcacgaag ttgaccctgg gaaactccag
      301 ggcgatagga tgtagcgcaa tcgggttcga gagccggatg tcaaacaaag atggcagccc
      361 cagtttcttg cgcacgccga cgcttagcac gcccatgtgg acgaaaacaa tggcgcgccc
      421 ggaggcaagc tcataaattg ggtgcagccg atcatcgctc actgaaaact tgtgcatggc
      481 ggggaacagg caaatgcccc gcaagcccac gttttcaatg gcgtgtttcg cgcgctgcaa
      541 cgcgtcctca gctgtgggat tcagcataaa atagccgaca aatttttccg ggcacgtccg
      601 gacagcaccc gccacagaat tctcgtcccc tggaaagctc ccgatcaata ccagccgctt
      661 caggtcgtac ttttccatct cctgcatcca gcggcctgcg agttcagcgg cgtccttact
      721 gggaatctcc caccctagtt gacgccccat ttcctccggc gtcctctcag tgcttaaatc
      781 ctcgaaaaaa gccctcgaca acagatgcac atgcgcatcg gcgataggaa gggttcccca
      841 cggtgttttc atagctttgg tggccgggcg atctgttgag ccgccttggc gccaaatgca
      901 ttgtactgcc caggacccgt gctgattcac agacgccatt cgaactcttg cgcgcctgtc
      961 gaaaagcagg ccctttcgaa aagacctctg cccggtcctt ccgaatcaca ccacttcttc
     1021 ttttatttcg cgagcatttt cagctctttt cggcctccat ccagtctgct taatcgacgc
     1081 ggctgctacg ctcgatccag tagaggggcg gatggcattg aaagccttcc tttgtctatt
     1141 tgcccagctc ttgtcccgga aacggtacgt cgccacgtat ctcaaccctc aactcccgct
     1201 tgctttcaaa agccccgctc ctctacaccg ggactaatcc acgagccaga atacgctcca
     1261 gatgttagca tcgaagagaa atgctatcgt ccgtctggtt tgcaactcta ggcgacgccg
     1321 agcgctgccg tctcgaagaa tcggatcctt tacctgcgtc cgccgacatc gccatcatcg
     1381 ggggcggcat gattggcttg gccacagcct actatctctg tgaagctggc gcaacgggta
     1441 tctgtgttat cgagcgagac acattggttg gggaagcgag cggagccaat gcgggcggcc
     1501 tctggtttgg ccagcagagc caggaaatgg gccctctttc tcccctggcg caggcgagca
     1561 gccttcttta cgaggaactg gcggcgcagc cgggctgggc cttcgatttt cggcgcaccg
     1621 ggctcttgga gttgcttttt gaccgggaaa gtctagccgg agcagacgct cgagcccagg
     1681 cggttcgcag agccggcttt cgcgccgaag tggccggccg cgaagatctt ggaaagctcg
     1741 agccgtccgt cggcgatgcc ccggagggcg cgattctcta cccggacgaa ggccaattgc
     1801 atcccgtcaa gctcggcgcc gcgtttgcgc gccacttgcg aaatcagaac gttcgcttct
     1861 cgctgcggac cgaggtgagc gaggtcggcg acgcggtcgt gcggacgtcc aacggcgacc
     1921 tccgcgctga catcatcatc atcgcctcgg gcgcctggac tcctttactg acgtgcaccc
     1981 tcgggtacag gcctccgatc aagccgatgc gcgggcaatt gctggccact ccacccagaa
     2041 caccttttct gcgccacacg gttattggcc gccgcttcta ttattggcag ctcaccgagg
     2101 gccatgtggc cggcggaggc acagtcgagg acgttggctt tgaacgcggc gtcagcgcgc
     2161 gggacctgac ctgcatacga gaggaaatga acacgctttt tcctgatctt tgcgcctcgc
     2221 cgacggcgct atcatggtca ggattccgcc catactgcga agacttacgg cccatcatcg
     2281 gccgcgtgcc gggctgcgag caaatctatg tcgcggcagg acatttcaag aaaggcatca
     2341 tgctcgcgcc ggttacaggc aaaattcttg cggatctgat cacaaccggg aaaaccgatt
     2401 tacctatcat ttccctggat ccggcccggt ttgcggctgg cggaagggcg tgaggtgtag
     2461 ggaacacacc caaaatctcg gctcggcccg ggctataccg cttgcctggg aagaagccag
     2521 ccgatgcggc gccgagctat aattgattca gacgcgcgag aacgaagggc cagcgtgatt
     2581 cgcggatcca agcagtcccg cactaggtcc gccaacctcc tatgtccacc ttagccgaaa
     2641 tattggccga gaatgctcgt cccggcgact tgtgggagcc gatcgagcgg aatcggatcc
     2701 gatgttttgc gtgcggtcat cgctgcccga ttccggaagg acagtcgggc gtgtgcaagg
     2761 tgcggttcaa ccgcggcggc cagctgtttg tcccatgggg ttatgtcgct ggagtacaat
     2821 gcgaccccat cgagaagaag ccattcttcc acgtgcggcc cggcgccctg gccttcagtt
     2881 tcggcatgct gggctgcgat ctgcattgca gctactgcca aaactgggtg acctcgcaag
     2941 caatccggga tcccaatgcc gtggcgcccc cgttgcggac gacgcctgag caactcgtcc
     3001 gcctcgccat cgaggaaggc ggggaggtca tggtaagcac ttataatgag ccgctgatca
     3061 ccgccgaatg ggcggtcagc gttttccaag cagcgcgtgc ggccggcctg ataacgggat
     3121 tcgtctccaa tggcaatgca actccagaag tcctggagta catccggccc tgggtggatc
     3181 tttacaaggt ggatttgaag agcttcgacg atcgccatta ccacgaactt ggcggccgcc
     3241 tccagccgat tctcgacagc atccgccgca tttatgcgat ggggttctgg ctggaaatcg
     3301 tgacgctggt gatccccggc ttcaacgact cgaacgacga gctaaccagg atggccgagt
     3361 tcctcgctgg catttctccg gacatcccct ggcaccttac ggcgtttcac cgggactata
     3421 aaatgacaac ccccgaggac acctcgccgg acacactcgt gcgagctgcc gagatcgggc
     3481 ggcgcgccgg cctgcattac gtctatgcgg gcaatcttcc ggggatggtt ggcaacctgg
     3541 aaaataccta ttgcgccgct tgcggcgaac ttctgattgc ccggcacggc tatcgagttc
     3601 agaaatataa agtcacccct aacggcctct gccccaagtg cgcggctccg gtgcccggcc
     3661 ggtgggccga agcttttcgc aggcaaatta ccgatcatcc attcctgccg gtcctcgctg
     3721 gaccctaacg gcgcaaaaaa taacagggcg atcgatttca ggaggcgccg caagatctcc
     3781 ggccggcgcc aaggatgttc ggccagcaga tcgttgacgg acgcgcgacg gcttgctcca
     3841 gtaatgccgc cagcagccgc gcgtgatgat cccaggtgta ctggctgatg atggtttccc
     3901 gcgccgccgc gcccagccgc atccttctgt tgggatcttg cgccaacgag agcacctggt
     3961 tcacgaagtc atcggggcgc gccggatcgt agagaacacc gttgtaatca tgacgaatca
     4021 cttccgcgac ttgccccagc cggggagcga ccaccgcgcg gccgcaggcc atgtattcaa
     4081 agattttgag cggagaaaaa taaaagacat tctgggccgg atagggcgcg aggagaatat
     4141 ccatagccgc cagcatacct gggacgcact tccgtggcag caagccaaga aatgttaccc
     4201 gtgaggcgca gccgtttgct tcccaccgct tgcgaagcgc ttcggctgcg gggccgccgc
     4261 cggcgaaaac aaaatggacc tcaggatgcc gcgccgagat gcgaagcacc gcctgttgca
     4321 ggagatccag accatggaac ggggcgaaac tgcccgcgaa gccgatcaag aggcgcgccg
     4381 ggaggcgtcg cctcaactcc tggtccgccg cttctgggcg gaaaaggttt gtgtcagcgc
     4441 cgttcgggat cacggccatg cgctcgcgag ggagcccgcg cgcgagaaag taatcccgca
     4501 gaacgttcga taccatcatt aatcggtcag ccgcgcgcag tgtgcggtct tcgatccact
     4561 cggaaacacc tgggaggagg tgtacttggg ggtggaatcg ccgcgtctcg aacggcaccg
     4621 acgcgttcac ttcgaacacg atcggaattc ccagacgccg tgcggcgatc aggcttgaaa
     4681 accggtgagc gtggaaagtg agaaccacgt ccggccgctc gcgccgcagg atattgatct
     4741 caaggggaac tccacgaacc gagcgccagt agttggctgt ttcccaaagc cagttgtgcc
     4801 ggtggccggg agctttcact gcattccttt gagcttgctt tcgatgccgg tcgatcacaa
     4861 cgactttatg gcccaaccgt tccagagcgg cggatgtttc ggcgatctgt gtggcggagc
     4921 cggtcgagcg ctccaggtcc cactcgtagt taaaataaac gatgcgcata ccgtgtcagg
     4981 ccggccaatt tctggcaacg gcatctaagg cctcgcgact ctcacgggcg gggacgcttc
     5041 gggaccgggc ggcgtaaatg ccatccagga gccgctccac atgctcgaaa gaatacatgg
     5101 cgcggacccg cgaccggttc cagttcccca tcttttcgcg gcgtcctggg tcaccgccga
     5161 ggtcgagaac ggcccgggcg atgccttcaa aatcgccaac ggagaccagc acgcctccgc
     5221 aaccgtagcc cagcatttca ggaagcgctc cgacgggagt tgcaattacg ggcagcccgg
     5281 cggccatggc ttccagcacg gagttcggca tgccctccgc ccgggatggg agcaggaaaa
     5341 tatcggcgtc ggccagcagg ttccattttt cctgtgagga gaccgggccg agaaatcgga
     5401 cattggcctc aacgcgcaat tcggcggcgc ggcgtctcac ttcctggcct tcgccgaagc
     5461 cgctggtctg gtcgggtccg gctaagagaa aacaaaccgc ctcgtcgcga agcagcttgg
     5521 cggcgctgag aatgtcgaaa tgacctttat ggctcccgag cgtggcaaga gtcaagactc
     5581 gcagaccgta ccggaaactc gtgctcgtag gccggatctg cggcacgcgc actccgttag
     5641 gaacaacatg aatccgggaa tcgggaaccc actgggcgag cagatcgcgc caataggtcg
     5701 aaagcacgat gatctcgctg gcggcgcgca aagagcatcg gatcgcccat ctgcttacgg
     5761 tccttcccct ggcgtaccag tgcccgaatt cgccgctgtg gatctgcaat agcgcccggc
     5821 gcctcaaggc cctgctgatc aagacgtaga tggccgactc ggcaaaattg accccggacg
     5881 acgacttgat atgaacaata tcaggccgca gaaaggcgac agccgcggaa aaaccgatga
     5941 agtctgcgaa ccggcgagcg gccctgagcg cccgccaatt cgggaaaaac gtgtattgct
     6001 ggcggcgcca caagcggatg gagtagcgtt tggcaagcgc ggaccgcagg atcatttgca
     6061 cgcccgtttc aacgcccccg gtggggagcg cagacggccg cggcgccacc agcagcacct
     6121 tgggggaagc ttggctgggc ctggcgagga gcttcttgta cgcctccaga agcggctcgc
     6181 cctgcgactc ccagcttctg tgttcactca cccactcgcg cgcgctccgg ccgagccgga
     6241 taagagactc cggatctgcc atcagttcct caatgcgccg cgccaaagcg cctgcggaac
     6301 cggctgggaa aagaacgccg ttcacgcccg gcgcaaccaa ttcccggtgc cctccacagt
     6361 cgcttgcgag aagcggcttg gcataggcca tgatctccaa aggcttgagg ggggtaacaa
     6421 ggtccgtgag gcggaggctg cggcggggca ggataaataa gtcaatttca cggtacaggg
     6481 gtgcgacttc ctcgtgcggg acgccgcctg taaatcgaac tgccagacca aggcccaggc
     6541 gccgcgcctg ctcctccaac gccggccgtg cgctgccgtc gcctgcgatc agtaggcgga
     6601 gattcgggtg cttcaaggtc aacagcgcgg ccgcctcaat caacaaatcc agcccctcgt
     6661 agggaacgag agttccgata tagccgatcg tgaattcttt gcccgagacg cgattcgatg
     6721 ggaggccggg cctatgcacc tgtatatcca cgccattgcc ggcaatggtg attttgcccg
     6781 gggatatgcc tcggcggatc atctcctctc gcaaggcttt gcaaatcacc accacatgcg
     6841 aagcgagccg gcaggcgagc aggtcctgaa gaagataagc gccgcgcacc aacactcctg
     6901 cgccgtcaag accctcctcc atcagcgacc gaatttcgta aacgacaggc agcccaagcc
     6961 cgcgtgcggc tgcggctgcg gccaggccgc aaaaggagag gctatgcgcg tgaatgatat
     7021 ctactcgctc atccaaagcc agacggcgaa tccggcgcac aaacgcaggg aaagctgcga
     7081 acttaatcgc acgttcccac aaggatttgt gtgcagccat gaagcgcgag ggatcgcacc
     7141 cgttgtagca gcggtgatag gcaatgccat tccagtattc gacgccacta gtctgagccg
     7201 ggtccgcagc gcgctggaaa ggggcgctca aaacgacagg ctcgagacca aggcgccgct
     7261 gggcctcgac cgtatagcgc gtgcgaatcg cgccgccggt gacatcgggc cacgtattgt
     7321 acgccacgtg caagactttc atcgctcaaa acaggccagg ccgcccgcgg gctgaaagcg
     7381 gaatcgcgga gctggcggct cgatgctcca ctccacggtt tcttctccgc gctggcagaa
     7441 gatgcagtcg ccctgccgcc atgtctgcgg gatctcggtt gcatcgccgc tggaaaccga
     7501 ttcggtgaac ggtgagatga aggtaaacca gcaaaggggc agatccgcgc tgcgctccag
     7561 gctgatgacc ggggatggct cccactggcc gaagcgccgc gaccagattc ccttctcgag
     7621 gcgccagccg ggttccgaga aacagaagcg gagtcccgcc ccaatttgcc gatcgacggc
     7681 cgaaacaacc tgccagcctt cgctgcgaat ttcaactcca ggcggaaaat gaaatcgaag
     7741 cgcgcaatga tgccggccgg cgccttccag acggtcaatc acaatccagc aagagcgatg
     7801 cgcgggaggg cgcaggaaaa gcaccgcgcg ataatgggtc acgggcttcc gaagccggtg
     7861 atagccttcg tgcatgccgg ctgcgaagtc atactgcgcg ccggaaaata tatccagcaa
     7921 acgagtgggg caaggacgca gccatttgaa ggtgtcgaga gcctcggttt gatccagata
     7981 gtccaccgta acggtgttgt gggccgccgt gccgcggagc gaatcgcgaa tgcgccgatt
     8041 tgcctcgtaa acgcccgttc cggggtcggc caggatccag cggccgcgga gcttgatctg
     8101 cacacttaat gcgtcggcgt ggccatgcga tgcattaggc caaaggccga gcggcccgca
     8161 gtcgaacagc agcgcaatcc ggtctcccgc gtcctcggcc tgccacaagc tgtagccgcc
     8221 ctcccggaat gcccgtgaaa tggctgtgtt gtgattctgc tgcggcgcct gaagagcctc
     8281 aagaccctcg atccatacat ccgcatcacg cttgtcaaat ccttcccttt gtttggaaag
     8341 ctctggaaac ggtatgccgc ggccatcgtc gctatcgccg atgcgcgccg gatctcctgc
     8401 ctcatccgtc aaagcagcga gaaagtcgcg catcgccgcc atgcgctgcg gaattaccgc
     8461 aggcagctct cgtcccgcgt tccgtgcgat cgcggccgcc tgtgcgtaaa aatccaatat
     8521 gaactgctga tagccggttg attgctctct gccgataccg tcacaggtca cctggcgctc
     8581 gatctggtca gaaagaatct cgatcgcccg ctgctcctgc cgcttcgctc cgggcaaatc
     8641 ggaaaacgcc acgctgagca tccacagcgc cgctgcttcg ccgatcaggt gattggttgg
     8701 atcggcataa agcgacaggt gcgatacaag atattgataa tgctccccca ggcaaccgag
     8761 ccatcttcgc agcagcgtct catcgaggaa atcgcaatcc aacagcaaag caacggccca
     8821 cagccaggaa accgctcgaa tccccac
//
LOCUS       DASREK010000168         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1070422, whole genome shotgun sequence.
ACCESSION   DASREK010000168 DASREK010000000
VERSION     DASREK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070422"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1051)
                     /locus_tag="VFA54_08645"
     CDS             complement(143..1051)
                     /locus_tag="VFA54_08645"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HZS50914.1"
                     /translation="MFFLAAAGAFAIGIPMCAQQQEMTARDMFESAAGLLVPAPAAKP
                     AKTPKSKTAKTAAARTSPGAKAEQAKSPPASKSSGAPAAAITAGQGEVVPVSLRSGAA
                     APLGLRYSILRQVQGNQTEEVNADSTFHSGDRIRLSIQANDTAHLYIVQRGSSGNWSV
                     LFPSPEIANGSNLIERGKQYEVPNGQWFAFDQQAGEEQLFIVLSRKPEADLENVIYSL
                     RQGTKTSPAAPREQAGATILAENRPPIGDDLVSRVRSQVFARDLVFEKVDENTPGEKK
                     EKAVYVVNKSGSADSRVVADVKLHHR"
     gene            complement(1056..2930)
                     /locus_tag="VFA54_08650"
     CDS             complement(1056..2930)
                     /locus_tag="VFA54_08650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HZS50915.1"
                     /translation="MPTRDQTLDLLASVIGDKYEVLQWIGGGGMADVFLARHRVHGGL
                     FAVKVLAEQLAGDRTIVERFLQEARTAASLSGHANIVPIFDIGEDQGLHYLIMQYVEG
                     EDLASYLEREGALAPVQAAQVILQVAKALVWAHSKGVIHRDLKPANIRLDRYGRVIVL
                     DFGIAKASDVPTALTSLGERLGTPFYMAPEQVRGDSCDARSDLYSLGVVFFELLTGRR
                     PFTGESYRAIEHAQMYTPPPSPGDINPSIPPEYSRVVLRLLEKDPGGRYQSATELLED
                     LKALDVGRATTTLEPLVAEEIRQWTEGAGSRTAPRADDAPSRRAATPSRPAPEPAPQR
                     GGGLKTWLIFAGGGVVIAAALALAIFSGHKPSRAGSTRPAEERAGAESVGRAAPELAP
                     QIETPTGSMELVPAGNFTFGDDAKPSPNPTRTVFLPAYYIDKTEVSNAAYKLFCDATA
                     HPAPEAPPWDAGYFLGKPNYPVVNVSGEDARAFANWAGKRLPTEEEWEKAARGSDARI
                     FPWGNSPPARQANLKGAADGYENTAPVDAFPEGASPLGPLNMAGNVWEWTASPYPVTA
                     REIADMKALLPSAGSRWFVIKGGNFTSEELWLRAYMRRGFPEDGKSPFIGFRCVKDAK
                     "
     gene            3179..>3606
                     /locus_tag="VFA54_08655"
     CDS             3179..>3606
                     /locus_tag="VFA54_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZS50916.1"
                     /translation="MPSHLQAPRSKRPAGSPHAYPGWKAYAALAAVSWLWGTTFLAIR
                     ITNEALPAALFACSRFLASGSILLGVCVLLGARLPRGRERLDALFSGALVLGVGSGGW
                     IFAEHYIVSGITALFAATTPLWMVAADAAFPGGRPIHPRTL"
BASE COUNT          572 a         1218 c         1099 g          717 t
ORIGIN      
        1 gttggagttt tcccgtttct ccaaaataat ctccatccgg cgcgggcttt gcgacggcgc
       61 ttgccccagc gcgggcgggc acggcagaca aagcgctgcc gccaaaagac ctccgcccgc
      121 aatggcccgc cgcatcccgc ccttacctgt gatgcagctt gacatcggct accacccggg
      181 aatcggcgct cccgcttttg ttgaccacat agacggcctt ctcttttttc tcccccggcg
      241 tgttctcatc gaccttctcg aacaccaggt cgcgcgcgaa cacctggctg cgcacccggc
      301 ttacgagatc atccccgatc ggaggtctgt tctccgcgag aattgtcgct cccgcctgct
      361 cgcgcggcgc tgccgggctc gtcttcgtcc cctgccgcag cgagtaaatc acgttttcca
      421 gatcggcttc cggcttcctc gatagcacga tgaaaagctg ctcttcgccc gcctgctggt
      481 cgaaggcgaa ccactgtccg ttcggcactt catactgctt gccgcgctcg atcaagttgc
      541 tgccgttggc gatctccggc gatggaaaca gcacgctcca gttgccgctc gatccgcgct
      601 gcacaatgta caggtgagcg gtgtcatttg cttggatgct cagccggatg cgatccccgg
      661 agtgaaatgt gctgtccgca ttcacctctt cggtctggtt gccctgcacc tggcgcaaga
      721 tgctgtagcg aagcccgagc ggagccgccg cgccggaacg gaggctgacc ggaaccactt
      781 cgccctgtcc ggctgtgatg gccgccgctg gcgcgccgct ggatttggag gccggcgggg
      841 atttggcttg ttcggctttt gcgcccggcg acgtccgggc cgccgccgtc ttcgctgttt
      901 tcgatttcgg cgtcttggcc ggtttcgccg ccggcgccgg caccagtagt cctgccgcag
      961 actcgaacat gtcgcgcgcc gtcatttcct gctgttgggc gcacatcggg atgccgatcg
     1021 cgaatgcgcc cgccgctgcc aaaaaaaaca tcattttatt tcgcatcttt gacgcacctg
     1081 aagccgatga atggggattt cccatcctcc ggaaagcccc gccgcatgta agcgcgaagc
     1141 catagttctt cgcttgtgaa gttgccgcct ttgatcacga accatcggct gcccgccgag
     1201 ggaagcaggg ccttcatgtc ggcgatctcc ctggcggtga ccggataagg gctggccgtc
     1261 cactcccaca cattgcccgc catgttcaac ggccccagcg ggctggcccc ctccgggaat
     1321 gcgtccaccg gagccgtgtt ttcgtaaccg tcggcagcgc ctttcaggtt ggcttgccgt
     1381 gcaggcggcg aattccccca gggaaagatc cgcgcgtccg agccgcgcgc cgccttttcc
     1441 cactcctctt ccgtgggaag ccttttgccg gcccagtttg caaaagctcg ggcgtcctcc
     1501 cccgagacgt tcacgaccgg gtaattcggt tttcccagaa aatagccggc gtcccaaggg
     1561 ggcgcttccg gcgctggatg cgcggtcgcg tcgcagaaca gcttgtaggc ggcattggaa
     1621 acttcggtct tgtcgatgta ataggcaggc agaaaaaccg tccgcgtcgg gttgggagac
     1681 ggtttcgcgt cgtcgccgaa ggtgaaattg ccggccggaa ccagctccat gctgccggtt
     1741 ggcgtttcga tttgcggggc aagctcagga gccgccctgc cgacgctttc tgcgcccgct
     1801 ctctcctccg ccgggcgcgt tgagccggcg cgggagggct tatggccgga aaaaatggcc
     1861 agcgccagcg ccgcggcgat gacaacccct ccgccggcga aaatcaacca cgttttcaag
     1921 ccgccgccgc gctgaggcgc gggttcggga gcaggccgcg agggcgtcgc agcgcgccgg
     1981 ctgggcgcat catcggcccg gggcgccgtt cggcttccgg ccccctctgt ccactgccgg
     2041 atctcttcgg ccaccagcgg ctcgagtgtc gtggtcgccc ggcccacgtc aagggctttc
     2101 aagtcctcta gcagctccgt ggcggattgg tagcggccgc ccggatcttt ttccaggagc
     2161 cgcaaaacca ctctcgaata ctcgggtgga atcgacgggt tgatgtcgcc cggcgaaggg
     2221 ggcggcgtat acatttgcgc gtgctcgatc gcgcggtagc tttccccggt aaacggcctc
     2281 ctgcctgtca gcagctcgaa gaaaacgacg ccgagggaat acagatcgct gcgcgcgtcg
     2341 cacgaatcgc cgcgcacctg ctccggggcc atgtagaacg gcgtccccag gcgctccccg
     2401 agactggtca gcgccgtcgg tacatcgctg gccttggcga tgccgaaatc caggacgatg
     2461 accctgccat accggtccag ccggatgttg gccggcttca aatcgcgatg tatgacgccc
     2521 ttggaatgcg cccagaccaa ggctttcgcg acctgcaaga tgacttgcgc cgcctgcacg
     2581 ggcgccaggg cgccctcccg ctcgaggtac gaggccagat cctcgccctc cacatactgc
     2641 atgatcaggt aatgcagacc ttggtcttcc ccgatgtcga agatgggaac gatattggcg
     2701 tggcccgaaa gcgaggcggc cgttcgcgct tcttgcagaa accgctccac gatcgtgcgg
     2761 tcgcccgcca gttgctcggc cagcaccttg acggcgaaca atcccccgtg cacccggtgc
     2821 cgggccagga acacgtccgc catgcccccg ccgccgatcc actgaagcac ttcgtacttg
     2881 tccccaatca ccgaggcgag caaatccagg gtttgatccc gggtgggcac ggcaccctct
     2941 attctagccg cagcgtgcgg gaaaatacag cccgaaacat agctgccggg ttttggttga
     3001 gccgggcggg agcgccggat gctccacgaa aagaacataa gacccgtgcc gcgaacgcgg
     3061 agacataaga cttcctaatc gctgggggtt cgtatcaggc tcatcctcct gtcttcgcgg
     3121 aggatgccgc tgggctgttt ccggcgaagc tcgggcaggc tgctagaatc gtcggttcgt
     3181 gccttctcac ctccaggctc cccgctccaa acggccagcc ggctcgcccc atgcttaccc
     3241 cggttggaag gcgtacgcag ctctggcggc tgtctcctgg ctctggggca cgacgtttct
     3301 cgcgatccgg atcaccaatg aagccctgcc cgctgcgctt ttcgcctgct cgcggtttct
     3361 ggcctcggga tcgattctgt taggggtatg cgtactgctg ggagcccggc ttcctcgcgg
     3421 gcgagagcgg ctggatgccc tgttctccgg cgctcttgtc cttggagtgg gcagcggcgg
     3481 gtggatcttc gccgagcact atatcgtcag cgggattacc gccctgttcg cggccaccac
     3541 tcccctctgg atggtcgctg cggacgccgc cttcccgggc gggcgtccga ttcacccgcg
     3601 aaccct
//
LOCUS       DASREK010000169         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_909670, whole genome shotgun sequence.
ACCESSION   DASREK010000169 DASREK010000000
VERSION     DASREK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909670"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1811)
                     /locus_tag="VFA54_08660"
     CDS             complement(<1..1811)
                     /locus_tag="VFA54_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS50917.1"
                     /translation="MRFTILLIAALCWPAGLVLAQHPIGEGLGGPEKNPLAGDAAAIR
                     AGKKLFADSCEVCHGGDAKGGRGPALAAGVFQHGSEDEQIFENIRNGIAGTQMPAFNM
                     LKAEIWQLVAYIRSLSGTVTEETVPGDPAAGEKIFTGKAGCAACHQVNGSGGRIGPDL
                     SAVGRWAAKSLREAIVNPNGRDGRRPNVVAVKTRDGRQIRGIRKNEDTTSVQLMDAAE
                     QFHLLDKKNLIEVRYEPDSLMPGDYGRRLSEADLQDLIAYLKTLRVRDLAKVAAAPQN
                     GGLDYERIRHSEREPQNWLTYWGDYQGRHFSALKQIDASNVRLLQARWAAQMPGTGSL
                     EITPLVIDGVMYTTGLPGTVFALDARTGRQIWKYQRSLKIVNPYAGNGVNRGVAVLGN
                     RVFFVTLDAFLVALDAKTGLPLWETQMGDPREGYSGTMAPLALKDKIIVGIGGAEYGI
                     RGFIDAYDPATGKRLWRFYTIPGPGEVGNDTWEGDSWKRGGASAWMTGTYDPDSDTLY
                     WGVGNPGPDLNGDVRKGDNLFSCSVVALDPNTGRRKWHFQFTPHDTHDWDSAETPVLV
                     DRLFRGERRKLMLHADRNAFFYVLDRTNGKFLLGKPFA"
     gene            complement(1834..>2309)
                     /locus_tag="VFA54_08665"
     CDS             complement(1834..>2309)
                     /locus_tag="VFA54_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HZS50918.1"
                     /translation="AQGIVGGEMAWPLVGMGMLLGFALIMVEVRSPMLFAVGMYLPVG
                     TTFAIFAGGVIRWIADRAAAGRGFNEAQRTRVENAGVLTASGFIVGEALMGLVVAGIR
                     IGEEVTKRSLMPVIFKEPSYLGGFAVMVLLAAVMVRAPLANAGSPDEPAPPAAMM"
BASE COUNT          425 a          752 c          695 g          437 t
ORIGIN      
        1 gcaaatggtt tgccgagaag gaatttgcca ttcgttcgat caagtacgta gaagaatgcg
       61 ttccgatcgg cgtgcagcat cagcttccgc cgctcgccgc ggaaaaggcg gtcgacaaga
      121 acgggcgttt ccgccgaatc ccaatcgtgc gtatcgtggg gcgtgaactg aaagtgccat
      181 ttgcggcgcc cggtattggg atcgagagct accacggagc agctaaagag gttatcgcct
      241 ttgcgcacgt cgccgttcaa gtccggcccc gggttgccga cgccccagta cagcgtgtcg
      301 ctgtcgggat cgtaagtgcc tgtcatccat gccgatgcgc ccccacgttt ccagctatcg
      361 ccttcccatg tatcattgcc gacctccccg ggtcccggaa tcgtgtagaa gcgccagagc
      421 cgcttgccgg tcgcgggatc gtaagcgtcg ataaaaccgc ggattccgta ttcggcgccg
      481 ccgatgccga cgataatttt gtcttttaga gcgagcggcg ccatggtgcc gctataacct
      541 tcccgagggt cgcccatctg cgtctcccat aacggcagcc ccgttttggc gtcaagggcc
      601 accagaaacg catcgagagt gacgaagaaa acgcgattgc ccagcacggc cacgccccgg
      661 ttcacgccgt tgccggcata aggattcacg attttcaagc tgcgctggta tttccaaatc
      721 tggcgtcccg tgcgggcgtc gagagcaaac actgtaccgg gcagtccggt tgtgtacata
      781 acgccgtcga tcaccagcgg cgtgatttcc aggctccctg ttccgggcat ctgagccgcc
      841 cagcgggctt gcagcagccg gacgttcgag gcgtcgatct gcttgagcgc cgagaaatgc
      901 cggccctgat agtcgcccca gtacgtgagc cagttttggg gctcgcgctc ggagtggcgg
      961 atgcgctcgt agtcgagacc cccgttttgc ggggccgcgg cgaccttggc caggtcccgc
     1021 acccgcaggg tcttgaggta agcgatcaag tcttgcagat cggcctcgct cagccgccgg
     1081 ccgtaatcgc cgggcatcag cgagtcgggc tcgtagcgca cttcgatcag attcttctta
     1141 tcgagcaagt gaaattgctc ggctgcgtcc atgagctgga ccgaagtggt gtcttcgttc
     1201 ttgcggatcc cgcgaatctg ccggccgtct cgcgtcttca cggccacgac attcggccgg
     1261 cggccgtcgc gcccgttggg attgacgatc gcttcccgca aagacttcgc ggcccagcgg
     1321 cctacggcgg aaaggtccgg accgattctt cctccgctgc cattcacttg gtggcaggcg
     1381 gcgcatcccg cctttccggt gaagatcttt tcgccggcgg caggatcgcc tggaaccgtt
     1441 tcttccgtga ctgtgccgct caggctgcga atataggcca cgagctgcca gatctccgcc
     1501 ttcagcatgt tgaacgcggg catttgggtg ccggcaatgc cgttgcggat gttttcaaaa
     1561 atctgctcgt cttcggagcc atgctggaag acgccggcgg cgagggcagg gccacgcccg
     1621 cctttggcat cgccgccgtg gcaaacctcg caggaatccg caaaaagctt cttgcccgcg
     1681 cggatcgccg ccgcgtcgcc agcgagggga tttttttccg gcccccccaa tccttcgccg
     1741 atcggatgtt gcgccagcac caagccagcc ggccagcaca gggccgcaat cagcagaatc
     1801 gtaaaacgca tgcacggctc cttgacgtgg agactacatc atagcggccg gcggggccgg
     1861 ttcatcggga gagccggcgt tggccaacgg ggcccggacc atcaccgccg ccagcagcac
     1921 cataaccgcg aacccgccga ggtaggacgg ctccttgaat ataactggca tcagggagcg
     1981 cttagtgact tcttcgccaa tccgaatgcc ggctacaacc agccccatca gcgcctcgcc
     2041 gacgatgaac ccggaagcgg tgaggacgcc cgcgttttct acacgcgttc tttgggcttc
     2101 attgaaaccc cggcccgcgg ccgcgcggtc ggcgatccag cggatcacgc cgccagcgaa
     2161 aattgcgaac gtggtgccca ccgggaggta catccctaca gcgaacagca tggggctccg
     2221 aacctcgacc atgatcaggg cgaagcccag cagcatgccc atcccgacca gcggccaagc
     2281 catctcgccg ccgacaatgc cctgcgcca
//
LOCUS       DASREK010000170         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1407745, whole genome shotgun sequence.
ACCESSION   DASREK010000170 DASREK010000000
VERSION     DASREK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407745"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1114
                     /locus_tag="VFA54_08670"
     CDS             164..1114
                     /locus_tag="VFA54_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZS50919.1"
                     /translation="MNPSQNVREPRREIRLSGSLDAVRSSKIVVPTIYGQGGQQTLTR
                     LIPNGARVKQGDIVAEFDPTQQLDNARSAQGSYEDLGHQVEQKAAQNRADYEYRQSTL
                     TQAQAALDKALLELQKGPILSEIARLENEVKAEIARKHVASLKESNAARDRADAAALR
                     LLELQRDRQKIALERWQNNIAKLQVHAPLAGMAALQIIYRNNTITHPQEGDQLYRGQP
                     LVSIFDPSEMLVRCAVGEPDGTALAGGTRAKVYLDAYPDLVLPAHFESASPVASSNLG
                     SPIKTFTAIFKLEKTDPRLMPDLSAAVVLEAPDADGGNSK"
     gene            1111..2358
                     /locus_tag="VFA54_08675"
     CDS             1111..2358
                     /locus_tag="VFA54_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS50920.1"
                     /translation="MKVRRQKFRLLAGLVGVLAIIGAASAGAYRLRQIQPTVAGLPVA
                     PARKGDFLVIIRCRGELKAGRSAELYAPVVPNLRISWLASPGEVVQQGQPVIKFDSSS
                     AEQQLVQKEAALRQAQATLDQAIAQEKTTAEQDASDLADAKARVETARLEASKAEIVS
                     RIQGEENQIDLGIAEQSLKREEAVVALHVAASKARIASVTRQLRLNQADVDITKTRLA
                     QMVIRAPLGGFVVIEPNYNQWPNTQPFREGDNVFSGMNLAEMPDLSTLMMDAKVEEID
                     RGRIAQGNAARIHIDALPELDLPARINQISPMAELGIEMPPTRSFRAYAVLPHPDSRL
                     RPGMNGGVDIVVNRIANAISIPAKALFTRAGKPFVYLAGNGNYKLAEVQVQARNPDEV
                     AVSGIPAGAMVALADPNKGESAK"
BASE COUNT          497 a          752 c          767 g          433 t
ORIGIN      
        1 tgtccactaa aatcgtatca accaactctt ttcaatcggc gtctaatctg caagactgga
       61 cgcaatgata cggatcgcgg ccaaactggg ggcggggctg tgggcgatcc tcctggtatc
      121 ctgtagccgt caggccgcgc cgcgggttgc agaagcggcg tctgtgaatc cgtcccagaa
      181 cgtaagggaa ccccgtcgcg agatccggct cagcggctcg ctcgatgccg ttcgctcctc
      241 taaaatcgtc gtacccacga tttacggcca aggcgggcag caaacgctca cccggctgat
      301 tccgaatggc gcgcgtgtca aacagggcga catcgtcgcc gagtttgacc cgacccagca
      361 gctcgataac gcccgcagcg cgcagggaag ctatgaggat ctgggccatc aggtggagca
      421 gaaagcggca cagaatcgcg cggattacga atatcgccag tcgacgctga cgcaggccca
      481 ggcggctctc gataaggcac ttctcgagct acaaaaaggc cccattctaa gcgagatcgc
      541 ccgtctggaa aacgaggtaa aagcggagat cgcccgcaag cacgtcgcca gcctcaaaga
      601 atccaacgct gcccgtgacc gggctgacgc ggcggcgctg cggctgctgg aactgcagcg
      661 cgaccggcaa aaaattgcgc tcgaacgctg gcagaacaat atcgcgaagc tgcaggtgca
      721 cgcacctctg gccggaatgg cggcgcttca gatcatctat cggaacaaca ccattaccca
      781 tccgcaggaa ggcgatcagc tctatcgcgg ccagcccctg gtcagcattt tcgatccctc
      841 ggagatgctg gtccgctgcg cggtgggcga acccgacggc acggccctgg caggcggtac
      901 tcgcgccaaa gtctatctcg atgcgtatcc cgatctcgtc ctgccggcgc atttcgagtc
      961 tgccagtccg gttgcgtcct ccaatctggg cagtccgatc aaaacgttca ccgcgatctt
     1021 caaactcgaa aagaccgatc cacgcctgat gcccgatttg tcggcggcag ttgtgctgga
     1081 agcgcccgac gcggacggag ggaactcgaa gtgaaggtgc gccggcaaaa atttcggctg
     1141 ctggccgggc ttgtgggtgt gctcgcgatt atcggagccg cgtcggccgg cgcataccgc
     1201 ttgcgccaga tccagccgac ggttgccggg cttcccgtgg cgccggcgcg aaagggtgat
     1261 ttcctagtga tcatccgatg ccgcggcgag ttgaaggccg gccgatccgc cgagctctac
     1321 gccccggtgg ttcccaactt acgcatcagt tggctggcgt ctcccgggga agtggtccag
     1381 caaggccagc cggtgatcaa attcgattcc agcagcgccg aacagcagct tgtgcaaaag
     1441 gaggcggcct tacgccaggc ccaggcgacg ctggaccagg ctatcgccca ggagaaaacc
     1501 acggccgagc aggacgcgag cgatctggcc gacgccaagg cccgcgtcga gacggcgcga
     1561 cttgaggcgt ccaaggccga gattgtcagc cgcattcagg gtgaggaaaa ccagatcgac
     1621 ctcgggatag cggagcagag cctgaagcgt gaggaagcag ttgtggcgct gcatgtcgcc
     1681 gccagcaagg cgcggattgc gtccgtgacg cgccagttgc ggctcaatca ggcggacgtc
     1741 gatatcacca aaacacggct cgcccagatg gtgattcgcg cgccgctcgg gggattcgtg
     1801 gtcatcgagc cgaattacaa tcagtggccg aatacccagc cattccgtga aggagacaac
     1861 gtgttttccg gcatgaatct ggcggagatg ccagacttgt cgacgctcat gatggacgct
     1921 aaggtcgagg agattgatcg cggcaggatc gcgcagggca atgccgcccg tattcacatc
     1981 gatgcgctgc ccgagttgga tctgccggct cgcatcaacc agatttctcc aatggccgag
     2041 ctcggaatcg agatgccccc gacccgcagt tttcgcgcct atgccgtgct gccgcatccc
     2101 gattcacgcc tccggccggg tatgaatggc ggcgtcgata tcgtcgtcaa tcgcattgcc
     2161 aacgccatca gtattccggc caaggccctc ttcacgcggg caggtaagcc ctttgtctat
     2221 ctcgccggca atgggaatta taagttggcc gaggtccagg tccaggcgcg gaatccggac
     2281 gaagtggcag tctccggcat tccggccgga gcgatggtgg cgctggccga cccgaacaag
     2341 ggggagtccg cgaaatgaag cgcgtgctca ttccgctggt cgtgctggcc gttttgggcg
     2401 ctcttggctg gggctccctc cgcgtgatcc gtctgaccac aaatgcggg
//
LOCUS       DASREK010000171         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1241928, whole genome shotgun sequence.
ACCESSION   DASREK010000171 DASREK010000000
VERSION     DASREK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241928"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFA54_08680"
     CDS             <1..653
                     /locus_tag="VFA54_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50921.1"
                     /translation="AGKFLSSSREIRIVTNLCVYWDEIFLGDDVFQSGGLDSGPHAES
                     QTPRLTRLAPADADLRFRGFSAVAIDPQRRQPEHFDYAIVEPVSAWNPTPGLYTRYGD
                     VTPLVQAIDDCFVIMGSGDELRLRFSPAALPELRPGWQRDFLLFVDGWAKDADPNTAF
                     GGSVEPLPFHAMRGYPYPRGEHYPDDPLHNEYRRVYNTRQALELIRPLVAQKKRWN"
     gene            644..>2702
                     /locus_tag="VFA54_08685"
     CDS             644..>2702
                     /locus_tag="VFA54_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZS50922.1"
                     /translation="MELRSKIGRALFLGFLLLLVNSGYLRAFPDPNLFYIANVVIHIG
                     LGAAVFLVFWFWWTGKPLGAVTFLVAAAGITGFALAVVGAQTPFRWLVWLHISACAAA
                     AVALGMQWRTHGPPVLLRAFVAAAGVALILPVSASVKSHYWPDPASRIVNPKVVATSM
                     EEEGAGPQSPFFPSAAETSTGKIIPSDFFLDSKACAQCHKDIYEQWSSSMHHFASFNN
                     QFYRKSIEYMQDVVGTKPSKWCAGCHDHAVFFNGRFDRPIREQIDTPQAQGGLGCMSC
                     HSITHVRDTTGNNTFTLEYPPLHELTTNKNPIVRGLHYYLINTAPEPHRRAFMKPFMR
                     LDSAEFCASCHKVHLDVPVNQYRWFRGFNEYDNWQASGISGQGARSFYYPPKSSTCVD
                     CHMPLVASRDPGNHNGFVHSHRFPAANTAVPFVNQDNEQLRATEQFLKDGALSVDIFA
                     VSPEQAPSFGNIGSLEMRRRSSDTVQAASSFAVGEEAESTAVPAVIREVGRIAAPIDR
                     AAPPIHPGDTVRVDVVVRTRKVGHFFPGGTVDAFDVWLELKAEDENGRVIFWSGSVAD
                     EANGKGPVEQGAHFYRSYQLDEHGNMINKRNAWQTRSVLYVRLIPPGAADVAHYRLHV
                     PRDAKGAIKLTAKLNYRKFSYYYTQFSYAGRPEPGPVAAGFDDRTFLFRQADIPANVS
                     GKIK"
BASE COUNT          482 a          833 c          798 g          589 t
ORIGIN      
        1 tggccggtaa gtttttgtca tcctcgcggg agatccgtat tgtgacgaac ttgtgcgtct
       61 attgggacga gattttcctc ggcgacgatg ttttccagag cggcggcctc gattccgggc
      121 cacacgccga atcgcagact ccacgtttga cccggctggc gccggctgat gcggatttgc
      181 gttttcgcgg cttttcggcc gtcgctatcg atccgcagcg gcggcagccc gagcacttcg
      241 attacgcaat cgtggagccg gtttccgcgt ggaatccgac gcccggcttg tatacgcgct
      301 acggagatgt gacccctttg gtgcaggcaa tcgacgactg cttcgtgatt atgggatccg
      361 gagatgaatt gcggcttcgc ttctcgcccg cggcgctgcc ggagcttcgc cccggctggc
      421 agcgcgattt ccttcttttt gtcgacggtt gggccaaaga cgccgatccc aacacggcgt
      481 tcggcggttc ggtcgagccg cttccctttc atgccatgcg gggctatcct tatccaagag
      541 gggagcatta cccggatgat ccgcttcaca acgagtatcg ccgcgtctac aacacgcgac
      601 aggcgctgga gctgatccga cccctggtgg cgcagaaaaa gcgatggaat taagaagcaa
      661 gatcggccgc gccttattcc tcgggttcct gcttttgctg gtgaactcgg gctatctgcg
      721 agctttcccc gatcccaacc ttttttatat tgccaacgta gtgattcata tcggcctggg
      781 agcggcggtt tttctggtgt tctggttctg gtggactggg aagccgctcg gcgcagtaac
      841 tttcctggtc gctgcggccg ggatcaccgg atttgccctg gccgtggtcg gcgctcagac
      901 gccatttcgc tggctggttt ggctgcacat ctccgcctgc gccgcggccg ccgttgccct
      961 ggggatgcaa tggcgcaccc acggcccgcc cgtgcttttg cgggcctttg ttgcggcggc
     1021 cggcgtagcc ttgattctgc cggtaagcgc cagcgtgaag agccactatt ggcctgatcc
     1081 ggcaagccgc atcgtcaatc cgaaagtcgt ggcgacaagt atggaggaag aaggcgccgg
     1141 gccgcaaagc ccgtttttcc cctccgccgc cgagaccagt accggtaaga tcattccttc
     1201 cgacttcttc ctcgattcca aagcctgcgc gcagtgtcac aaagacattt acgagcagtg
     1261 gtccagttcc atgcaccact tcgcctcgtt taacaaccag ttctaccgga aatcgatcga
     1321 gtacatgcag gatgtagtcg gcaccaagcc ctcgaaatgg tgcgccggct gccatgacca
     1381 cgcggttttc ttcaacggcc ggtttgatcg gcccatccga gagcagattg atacgccgca
     1441 agcgcaaggg gggctcgggt gcatgtcgtg ccattccata acgcacgtgc gcgatacaac
     1501 cggcaacaac acgtttacgc tcgaataccc gcccctgcat gagctgacga ccaacaagaa
     1561 cccgatagtc cgcggattgc attattacct gatcaatacc gctcccgagc cgcaccggcg
     1621 cgcttttatg aaacccttta tgcgccttga ttcggcggag ttctgcgctt cctgccacaa
     1681 agttcatctc gacgtacctg tgaatcaata ccgctggttt cggggattta acgagtacga
     1741 caactggcag gccagcggca tttccgggca gggggcgcgg tcgttctact acccgccgaa
     1801 gtcttccacc tgcgtggatt gccacatgcc gctcgtcgcc tctcgagatc cgggcaatca
     1861 caacggattc gtacactccc atcggttccc ggcggccaat accgcggttc cgttcgtgaa
     1921 ccaggataac gagcagctcc gcgcgaccga gcagtttctg aaagacggcg cgttatctgt
     1981 agatattttc gccgtttctc ccgagcaagc tccctctttt ggaaacatcg ggtcgcttga
     2041 gatgcggcgg cgctcgtcgg atactgtgca ggccgcttcg agctttgccg ttggggaaga
     2101 ggccgaatcc accgctgtgc cggccgttat tcgcgaggtc gggcggattg ccgccccgat
     2161 tgaccgcgcc gcgccgccca ttcaccccgg cgatacggtg cgtgtggatg ttgtggtgcg
     2221 cacgcgtaag gttgggcatt ttttccccgg cggcacggtg gacgccttcg acgtctggct
     2281 cgagctgaag gccgaagacg agaacggccg cgtcatcttt tggagcgggt cggtcgccga
     2341 cgaggccaat gggaaaggcc ccgtcgagca gggcgcgcac ttttaccggt cgtatcagct
     2401 cgatgagcac ggcaacatga tcaacaagcg caacgcctgg cagacgcgca gcgtcttata
     2461 cgtgcgcctg atcccgccgg gcgccgccga tgtcgcccat taccgcctgc acgttccccg
     2521 tgatgcgaag ggcgctatca agctgaccgc gaagctcaat taccgcaagt tctcgtacta
     2581 ttacacgcag ttctcctacg ccggacgccc ggagccagga cccgttgcgg cggggttcga
     2641 tgaccgcaca tttcttttcc ggcaggcgga catcccggcg aatgtgtccg ggaagatcaa
     2701 gg
//
LOCUS       DASREK010000172        49362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1903388, whole genome shotgun sequence.
ACCESSION   DASREK010000172 DASREK010000000
VERSION     DASREK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 49362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49362
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903388"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VFA54_08690"
     CDS             <1..1043
                     /locus_tag="VFA54_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZS50923.1"
                     /translation="IDLYSLRSQGWSAFEFLDYAVRQQVEIVHFSESRFLGSLEASHL
                     RQVKEYAGRLGLSIEVGMGSVCPGSKRFIAAKGTAEEQLARMFEAAKLLGSPILRAYV
                     GSWEDRLAGESGSPRDQSAADPIEQRIEEAVRVLRRVRSRARDMGIRIAMENHAGDLQ
                     AREMKMLIEEAGTDFVGCCLDSGNPVWAVEDPHLTLETLAPYVLTTHIRDSAVWQESE
                     GIAVQWTAMGEGTIGIGRWIARFAELCPGKPLSLEIITTHPPRLHRCFDAAFWKAFPS
                     MPAWEFARFVKLAREGKPPTRVLPASGSPGSQEHQFCKAAQERMALEDSLAWCRRHFG
                     AFCRDCGNSDLS"
     gene            complement(1050..1682)
                     /locus_tag="VFA54_08695"
     CDS             complement(1050..1682)
                     /locus_tag="VFA54_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HZS50924.1"
                     /translation="MKMLVCLPAALAVSSFLLAAPGPEQPEIPQGAHLLLRMVNSVNT
                     RTAQEGDYVYLRTASPISVGGRIIVPVNSYVQGVVSHTRRSGRVAGRAELGIRLETLT
                     LPSGRVVKFSPRLSSVDSNESEQKVDRQENFIKQGPDTGRDARSIAILAGTGASIGGI
                     ADRSWSGAGIGAGVGSAVGLATVLASRGREVELRQGRTLDVTFDRPVALE"
     gene            complement(1889..2764)
                     /locus_tag="VFA54_08700"
     CDS             complement(1889..2764)
                     /locus_tag="VFA54_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZS50925.1"
                     /translation="MRWTSRGMAWLGATLFCAALAHSQQLPVRWDELTGADFIQAIQK
                     SQGACVLPIGILEKHGPHLPIGNDVINVRWAALHGAEQEYSIVFPEYYFGQIFEARHE
                     PGTVAYSPRLQLELLQETTDEMGRNGCKKILIVNGHGGNNNLLPYFAQTQLSSPRDYV
                     VYVYARPQYPPGRPPLKSKVDQHAGESETSHTMISRPDLVHMDRARSESGADLNRLDL
                     PAGVYTGIWWYAKFPDHYAGDGSLGNTALGEFDMKAWAADIANVLRAIKADQVSPRLQ
                     KEFFEKAAQPLNTRQ"
     gene            3021..3803
                     /locus_tag="VFA54_08705"
     CDS             3021..3803
                     /locus_tag="VFA54_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS50926.1"
                     /translation="MRPALLLFMAALAWGQQAEIRNPHTTPADVAAGARIFHSHCAEC
                     HGARGEGGRGPNLTTGVFFHGSSDADLFNNISNGIAGTAMPSLFFPPDGVWQVVAYVR
                     SLAQGGGEQRPSGDAARGAMLFREKGCLGCHLVRGEGGIKGPDLSVVGSQRNLEHLRQ
                     AIVDPNAEVPREYWVAKITLENGSSYTGFVMNEDTHTVQILDFTQGLKSLSKQSFRKF
                     DIDKSSLMPSYKGQLNDREINDLVAYLWSLKRESHAHEGRSE"
     gene            3800..5422
                     /locus_tag="VFA54_08710"
     CDS             3800..5422
                     /locus_tag="VFA54_08710"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZS50927.1"
                     /translation="MKGLRLALCALLAAGSVYGQLTFERLLKADSEPQNWLTYSGSYR
                     GWRYSKLDQINRQNVKNLKVAWVYQMPTTHRIEATPLVVDGVMYVSEPPSNVVALDPA
                     TGRQFWRYRRSLPSKVNVCCDQVNRGVAVLGDRVFVGTVDAHLVALSARTGAVLWDAE
                     VADYRSGYSITVAPLVVKDMVITGIAGGEFGIRGFLDAYDARTGQRRWRFWTIPGPGE
                     KGNETWSGDNWKHGGGPTWVTGAYDSAQNLIIWGTGNPSPDWNGDVRRGDNLYTDCAI
                     AVDADTGTLRWYHQFVPHDVHDWDAVQVPVLVDSEWRGRQRKLVYWAHRSGLYEVLDR
                     ETGEFLFGKPFAKVTWMSGFDEKGRPIRNPGVDPTEEGVYVWPGVQGATNWYSPSYSP
                     QTGLFYLTVWENKGFYQKGEADYKPGNLFIGSVPKIDVEEDPGYGAIRALNPKTGDKV
                     WEYKVHTKPWSGVLSTAGKLVFGASGGWINRDEREESESSFYALDAESGKELWRINLG
                     GNMSANPITYSVNGKQMVTMAAGSGIFTFALP"
     gene            complement(5518..6645)
                     /locus_tag="VFA54_08715"
     CDS             complement(5518..6645)
                     /locus_tag="VFA54_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50928.1"
                     /translation="MQSAFIIGVTEWFLTGMQECCAASGIGIYKTLSRHPAPSELFQL
                     LSVFSPEIVFLEVTYSRRELELLREIQTCYPKIATIGFGEPSDLPLPAGVHQYLAAPF
                     SPEAFQRALRAAAEFQKAAIPDNIFAFVPAKAGSGATTLALCVSRYLAQRCQKRVLLV
                     EADVHSGPISFLLDLKPEQSIVDALENCHRLDDTSWQRLVTKTDGFDLLPAPPAIQTG
                     HLMQWSCRRLLTYVRTRYDAVLVDLPEVVNDATEAMVTECRRVHVICTPEKASVLLAR
                     RRIYELAARGAKAHQLALVLNRQASDNGAAEQIEQLLGRQAALVVPNDYPAAQQVVRS
                     PGPIEELSRFGRAISSLARTIGGLPIPLERSRTPWFRRLSA"
     gene            7079..7531
                     /locus_tag="VFA54_08720"
     CDS             7079..7531
                     /locus_tag="VFA54_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50929.1"
                     /translation="MITVLIIGTSASLFICWLRCTVLLILRSMPARSCAHEAAEANGM
                     NFLDLRRRLYSLVSEESLPMLCEMLKKDLKLTKILLQQMGPETGGRISSDFLLTLNFR
                     LLGLRFAVLRRFRPGSARSALAEMSATLEYFSNCLSRRLALASQPARF"
     gene            complement(7720..10122)
                     /locus_tag="VFA54_08725"
     CDS             complement(7720..10122)
                     /locus_tag="VFA54_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HZS50930.1"
                     /translation="MGTPVRDQAEPGAAQRGALTRDELRRMDAYWRAANYLSVGQIYL
                     YDNPLLTQPLKLEHIKPRLLGHWGTTPGLNFIYVHCNRLIKKYDLNMIYVIGPGHGGP
                     GIIANTYLEGSYSEVYPNIGQNAEGMKRLFKQFSFPGGVPSHVAPETPGSIHEGGELG
                     YSLLHAFGAVLDNPDLLACCIIGDGEAETGPLAASWHSNKFLNPARDGAVIPILHLNG
                     YKIAGPTVLARISREELEQLLAGHGYKPYFVEGQDPEPMHQAMAAALETVLLEIQLIQ
                     GEARTKGFTRRPQWPMIVLRSPKGWTGPKEVDGVPIEGTFRAHQVPLDDFRAKPDHLK
                     ILESWMKSYRPEELFDEKGKLIPELAELAPKGQRRMGANPHANGGLLLKDLRMPDFRF
                     YAVDVPKPGAVAAEATRVLGRMLRDVMKLNGETRNFRIMGPDETASNRLNAVFEATSR
                     VSTAEILPSDDHVSPDGRVMEVLSEHMCQGWLEGYLLTGRHGFFSCYEGFIHIVDSMF
                     NQHAKWLKVTRQIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFIDLVVNKKADIIR
                     VYLPPDANSLLSVADHCLRSRHYVNVIIAGKQKAWQWLNMDEAIRHCTAGIGIWDWAS
                     NDQGGEPDVVMACAGDIPTMETLAAVDFFRQRMPQLKIRVVNVVDLMTLQPSTEHPHG
                     LSDQDFDSLFTTDKPVIFAFHGYPWLIHRLAYRRHNHDNMHVRGYKEEGTTTTPFDMT
                     VLNDLDRFHLAMDVIDRVPKLQSIAAHEKQFLRNKLIEHKLYVAEHGDDLPEIRDWQW
                     RG"
     gene            10382..10630
                     /locus_tag="VFA54_08730"
     CDS             10382..10630
                     /locus_tag="VFA54_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50931.1"
                     /translation="MATAMPGPEHREGRVARAIEQRTAKLPSDVFLWAALGSMAASLV
                     LQLMGERQRSNFVGQWAPSFLILGLYNKLVKLHGSERV"
     gene            complement(10996..12303)
                     /locus_tag="VFA54_08735"
     CDS             complement(10996..12303)
                     /locus_tag="VFA54_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS50932.1"
                     /translation="MAGWTFALRALRSRNYRLFFGGQSISLIGTWMTRVATSWLVYRL
                     TDSATLLGLVSFAGQIPAFFLGPLAGVWVDRWDRHRTLVATQVLSMAQSFALAALALS
                     GVIAVSHILWLSLAQGLINAFDMPARQAFVIEMVESREDLGNAIALNSSMVNAARLAG
                     PAIAGVIIAAVGEGYCFLLDGASYIAVIVSLLEMSMAPALTRRPQRRVLHELHEGWRY
                     VIQSVPIRSILLLLALVSLVGMPYSVLMPIFASSVLHGGPHTLGFLMAASGLGALAGA
                     IGLAMRRTVLGLGRRIALASALFGASLIGFALSHVLWLSLLLLPLTGFGMMQQMASSN
                     TILQTIVDDEKRGRVMAYYSMAFMGMTPFGSLLAGGLAARIGAPQTLILGGMLCLAGS
                     FWFFRQLAAIRDLVRPIYVQLGILPELAMGVQNASAFQTPPET"
     gene            complement(12508..13164)
                     /locus_tag="VFA54_08740"
     CDS             complement(12508..13164)
                     /locus_tag="VFA54_08740"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZS50933.1"
                     /translation="MTQDVEEAVAGRRRFIRRAVLAGAGGVAFLTARNAQADHVNNGE
                     DKRETRNTFLDIQRHENAHVAALLTALGASARPKPTFQNLVQPNFRQFAQVSQALENT
                     GVGAYLGAAPIIFNRGYLAAAGSIALIEARHAGWLNSLLQAPITANVYGMEQSFETPL
                     TPAQVATLASPFIASLNGGPPLDYAGTASAANDVAILNFALALEYLEAEFYNLNVANF
                     "
     gene            complement(13620..13697)
                     /locus_tag="VFA54_08745"
     tRNA            complement(13620..13697)
                     /locus_tag="VFA54_08745"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13659..13661),aa:Glu,seq:ttc)
     gene            13848..15056
                     /locus_tag="VFA54_08750"
     CDS             13848..15056
                     /locus_tag="VFA54_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50934.1"
                     /translation="MRRIPFWSILFGLLVASSLRAVEIATGMMLGLRYDPDAFYFRTA
                     ENPAWRKTYTGKGYRGEARGKLMNVRLAQALFEDEWLSERPFDAAANTARVIDALDFY
                     KRHGVLAISVGLQGADPGYAPNVNGITRQSGPQYGRSGGTLVSAFRPDGSLKPAWLAR
                     LESLLRAADQRGMFVCLIYFYPGLDVVFDSPETIEAGARQMTDWLIDHKFRNVIIDVA
                     DEWDAPGQKWDQSDYIPQNVGGMIERIRERFQAKRADFTLPIGASASGRMLYPASLAE
                     ISDVVLLHGNGRAPAEKADRAQQYKAYQRPLLMTGDDNGRETTLSHLAREQASCEAFF
                     RHGAGWGYTPWVQAERFPFYYLPGPSPDFTDQTPVAERDRAYFHAVLDAMARFVLEKP
                     PEGKKWKSKP"
     gene            15202..16017
                     /locus_tag="VFA54_08755"
     CDS             15202..16017
                     /locus_tag="VFA54_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50935.1"
                     /translation="MKTLYCAAMLALVTGTSWLIADTVILRDGRRVEGTFVGGDSRQV
                     RVLGTDGNLQTLDINDVESIRFSGNAAASAAPPASAPRPASSRPALVRERSEAVASQQ
                     PSVVVPAGTVITVRMIDSIDSDVNGAGERFRASLDDPLQVGEKIIAPRGSDVTVQVVR
                     VQQSGKLTGRDEVAVDLYDISINGRKYQVASSSAEVSSGSRGARTAKVAGGTAALGAI
                     IGAIAGGGKGAAIGAGAGAATGVGIEEVTHGQRVKIPAETRLEFTLKQPLAVY"
     gene            complement(16396..17082)
                     /locus_tag="VFA54_08760"
     CDS             complement(16396..17082)
                     /locus_tag="VFA54_08760"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZS50936.1"
                     /translation="MPFLLRLLLWGLAATAPQFAQSLLLGFKAGIPMTQYFDTRSAAS
                     LSSSADYSAATRRYTAGVSAEWRSETVLGIEADVLYKRMGYVGIIHTIDSSRGISTTA
                     AFDIKGDSWDFPILLKYRFRRKLHPYASGGGVVRYVGPVQARGEETAQDLVAGTTTRT
                     PIDTADPFDLRKRFYPGVSAGSGVELRRGRLRLLPELRYTRWTANIAKRGGLLRFEPD
                     QWEWLLGVLF"
     gene            complement(17264..19291)
                     /locus_tag="VFA54_08765"
     CDS             complement(17264..19291)
                     /locus_tag="VFA54_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZS50937.1"
                     /translation="MTKAISLFLLLSFSSAAAQIPEQDSRNTVIHDSNTHFQMPVFTS
                     REAWLERAAFLRKQILASAGLLPMPQKGPIHAEVFGKLEKNGYSIEKVLLETYPGFYL
                     GGNLYRPLGKQGPFPGVVSPHGHWAYGRLENQQLVSVPGRCINLALQGFVVFSYDMIG
                     YNDTNQVPHEFGSKRDELWSIGPMGMQLWDSIRAVDFVSSLPDVDPNRIAATGASGGG
                     TQTFLLMAVDDRIKAAAPVNMISASMQGGVCENAPNLRVGGNNDTSNMVIAALMAPRP
                     LLMVSASGDWTRNTPHEEFPAVQSIYRLLGAEQNVETIQIDQVHNYNKESREAVYTFL
                     GARLLGTKGPVTEKRFQVEQPQNLLALFMRQRPAGAITSLEQFISERIAEAKRGIDEL
                     HPHDRAALEKAREAFHERLTFSLLAAKPAAAEVISEKKEALAHGETLFVGRAGKGDRV
                     PAVWLTPVRPNPAVPPTLIVHPEGVAWVLSSSQTINGLVKGILDRGGVVMGIDAFQTG
                     SARAPRDRSKRAFTVFNQTDDANRVQDILTALAYLQGRSNAQTVNLVGMQMGGVWSYF
                     ARSMAGAGVNLAADLAQFGADSDEEYINKFFIPGLRKAGDFRAAAVLDTENKLLLYNA
                     SQDFPSDWVRDSAQAGGSVADLRPTAGEADLLAWIAPPELTPTGKRRAARR"
     regulatory      19419..19549
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            19582..21801
                     /locus_tag="VFA54_08770"
     CDS             19582..21801
                     /locus_tag="VFA54_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS50938.1"
                     /translation="MPVQNMSKATKVVVATTVMLSFISFWRAAAIVLCDLGSSAFYAG
                     GLAEGYVGKSAPWFILGIMLFSNAVLAVYIESCSMFVRGGVYRVVHEALGGTLAKFSV
                     SALMFDYVLTGPISGVSAGLYLAGLWNETLDLIHQKAFQVNTQHFAAVFTILVILYFW
                     RKNIIGMHESSQKALRIMQLTTVMVVILIGWCLLTIVKRGYNPVPLPTLENIHFSDEA
                     LGWLKGTIFPHITPIAILIGLGHSLLAMSGEESLAQVNREIASPKLKNLERAAFIIFI
                     YSLAFTSLVSFFGVMIIPDNVRPGYLDNLVGGLAMSVIGPLPLRLLFHGFVVLVGTVI
                     LAGAVNTALVGSNGVLNRVAEDGVLPDWFRQPHSKYGTTNRLISLVAFLQIFTIVLTR
                     GDITLLGDAYAFGVVWSFAMKALSVVVLRYKQPQGREWKVPLNFHFRGVEIPVGLGLI
                     TLVLFALAFTNLFTKKVATIWGFGFTVVIFAVFEITDHYKRRTEAAHTEELEKFRLEA
                     PGDLDVTSITVRPGNVLVAVRNPNQLEHLKKVLDKTDTRKIDIVVVTIKNVIRAGSGE
                     HDLTTEQVFTTDIAQLFSKVVSLAEKAGKHVELMVIPGSNPNEAIVETAQRLRSSLIV
                     LGSSGKMSPQEQAKAFGDAWEKLPRPRPQLSLEIYDAAKGESVYFNLGPHPPRLWPED
                     VDLLHSLWLELTEKGFGANLHHRDVVRVALRRLDEQLHSDHSNEVLEDVRREAMKGS"
     gene            22094..22552
                     /gene="rimP"
                     /locus_tag="VFA54_08775"
     CDS             22094..22552
                     /gene="rimP"
                     /locus_tag="VFA54_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041858220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZS50939.1"
                     /translation="MSTIAERVAEIAQRVASSEGMEVVEVEWKGGGNNRILRIFIDKP
                     SGVSHADCEIISQQVGTILDVEDVIPSRYTLEVSSPGLDRKLLKAADYQRFVGKKAKV
                     KLRHPIGGRTHFFGRLAGFEAGEVEMEAEGAGRIRFPLENVLTARLVAEF"
     gene            22639..24159
                     /gene="nusA"
                     /locus_tag="VFA54_08780"
     CDS             22639..24159
                     /gene="nusA"
                     /locus_tag="VFA54_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZS50940.1"
                     /translation="MATLYDSIETLSREKGIDAQIVLDAVKDAILVAARKYYKTAEDL
                     IAELNPKTGQIDVFAVKKVTEPVTNPLKEISLEDARRYDPNAQLDSEIRIAKPTEVLG
                     RISAQTAKQVIFQKIREAERETIFGEFSGRVGELVTCVVKRAEGPDLVVDLGKTEARL
                     PRREQSKAESFAPGDRIRAVIKSVDKFSRGPGVVVSRAAPELVMRLFEQEVPEIYDGT
                     VVIKACAREAGERTKIAVQSRDRDVDSVGACVGMKGMRVQSIIRELRGEKIDIIEYSE
                     DPVELATNALSPAKISRVAIIDPMEKHLEVVVDDSQLSLAIGKKGQNVRLAAKLMGWR
                     IDIKSEEEKRQEVESAMAALVSAGTPISVLLDHGLGEKLVEKLSEAGATTVERLSDMT
                     PEQLEEIPGIGPKMVEKIQLAVQSYYGQFEEEQGGGEAPAPESGTEEGQAENQAAAGA
                     EAGQEAASSVGEASGVELSASNAAPNESDKIALPGQSQAESVTAEAPGEAGGAERE"
     gene            24236..24331
                     /locus_tag="VFA54_08785"
                     /pseudo
     CDS             24236..24331
                     /locus_tag="VFA54_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
     gene            complement(24314..24508)
                     /locus_tag="VFA54_08790"
     CDS             complement(24314..24508)
                     /locus_tag="VFA54_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50941.1"
                     /translation="MRFWRFFDCGGRCRFYRRLLGRSGRFGLRRSTDSPASAILKVSS
                     YLLCDFVIERAGMRLLVGDT"
     gene            25202..27175
                     /gene="infB"
                     /locus_tag="VFA54_08795"
     CDS             25202..27175
                     /gene="infB"
                     /locus_tag="VFA54_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZS50942.1"
                     /translation="MHPTSRTPRVGPGVAPPPPGGAPAPDQRRPQHRTAYQRARDREQ
                     EQEAKILHRHTQQRTQVSEEPPPITREITISEGMTVKELSEKLGVKAGAVIKKLVDRG
                     IFATINQTLDSKTSTELAAQFGASTATTTFEEEVMQNVELAEEPKDLQPRAPVVTIMG
                     HVDHGKTSLLDAIRQTDVAAHEAGGITQAIGAYQVEHSGRKIVFIDTPGHEAFTRMRA
                     RGAKVTDLVVLVVAADDGVMPQTLEAIDHAKAAKVPILVAINKIDKPDAQPERVKQQL
                     ADRGLLSEDWGGDTVMVPVSAKAKTNLDLLLEMILLMADMQNLKANPNRPAMGTVLEA
                     KLDRGQGPVATVLVQNGTLHVGEYFIVGAVFGKVRALLNDRGEQIREAPPSTPVVVLG
                     LEDLPSPGDAFQVVTDTAKAKQIVTYRESKAREQAMAKSSRMTLESLHDQLRTGEVKE
                     LAIVLKADVTGSIEVLTDTLQKLSTDKVKINLIHSGVGAISESDVLLAAASNAIIVGF
                     NVRPERTAAVLAEKEKVDIRLHTIIYELTEEIQRAMAGLLEPVIKEVTLGKAEVRETF
                     RIPKVGVVAGCFVQDGRLTRDSEVRLLRDNVVVYTGKVASLRRFKEDVSEVKSGFECG
                     VSLSNFSDVKQGDVIEAFMTQKISGGDLRASSR"
     gene            27177..27470
                     /locus_tag="VFA54_08800"
     CDS             27177..27470
                     /locus_tag="VFA54_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HZS50943.1"
                     /translation="MPGQPTIGVLTLDLHIEHSHSLKDKRQVLRSLRDRLRGRFNVAT
                     AEIDHQDSWQRAVIAVVTLSGDRLYVEHTLQLAEKEAANLLGASLVAAEIEYL"
     gene            complement(27647..28120)
                     /locus_tag="VFA54_08805"
     CDS             complement(27647..28120)
                     /locus_tag="VFA54_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50944.1"
                     /translation="MQFQTGTPCGIGTNNDFAGVGEFGSFGVSNCPEGQFWVLNGPVT
                     INKGAFAGPVTTSASPRYFTVNVSPPPAGSFNLQHGVRDSIYAPGLQDWNIGLLKSFP
                     VTEKTFFQFRAEAYDFINHPNLSAPNWVPTSGQFGMITGKTSLARNLQLSLRFQF"
     gene            complement(28129..31170)
                     /locus_tag="VFA54_08810"
     CDS             complement(28129..31170)
                     /locus_tag="VFA54_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS50945.1"
                     /translation="MSINCLGLYMCSLRRVTARLFTAAILMLLTAGIVFAQLDYGSIT
                     GFVKDPSSAVVPGAKVTIRNEATGQERSTETNESGYYVITNLPPGVYTIMAEKEGFKK
                     FQSGQNRLQPNSTLSTDITLTLGATTETVEVTAAAATLQTESATVQKEVTGTQVLDQE
                     LNGRNPIFMVQLLPGVHGNNGITLGDLNFAATGGQSWQINGARVWDTLVTFDGAPAVR
                     TRSNGAIIGSADVDSTAEIQVLTADYAAEYGRAAGGQIRVVSKSGTRDFHGSLYEYFR
                     NSDLNANTWQRNLSPTTNFTTPFRYNNFGFAVGGPIWLNRKWREKLFFFIAEDWIRNR
                     NTDTQTQAVPTLLMRQGNFSELLGPNPFYSGTHLIYDPTTCPSLGAVSCQPFPNNIIP
                     QSRLSHNGIAILSAYPAPTPGFLQGNQNWIAQAAHPYNQRKDTLNIDIQATDRQHISG
                     RKSGAQYFEYQPFDQGSGETGKYFNRPNQTNTISWTYTVNPTLVNEARISFSLDDVYI
                     PVNASLPGFNRSVFGIDYPYLFGGKDIPGKIPTVNVPAPFYSLAGGPYPSHSSGPIWT
                     GGDSITKIWKNHTFKFGFSFEYSGENDGDQINVNTVPGGSNNQNGTFTFTDARTGLGA
                     TSGIGLANLALGLADSYTEIGPRAYTIWRGGMWEEFAQDSWKATQKLHIDYGLRITTI
                     QGFHPLWGNADYFDAALYNPAQAVQVDPKTGNVILGTGNPYNGVVIPGFSSFPSSAQG
                     RVLAASANICDGAPCSGLFAPNLSKSYIARQTALQPRLGIAYQLTAKTVVRSGIGRFV
                     TRMGLLDNIFPGGNSPFQPFVTVTNVSVDNPGAALTTGTAAPLTMTTLNPQLKPPEAW
                     NWNITIERQLPGDSLVSVGYVAHRGLHAWDVYDINQPLAGALQANPGINVNFLRPYKG
                     FAAIQEEESVVNSMYNSLQISFSRPFKNGLMLGVSYTLSKSMDGSSNYRDIVPDTYNT
                     SNLWGPSEYDTRHALLINYIYQLPFLKPARGFPGSCSEVGK"
     gene            31307..32647
                     /gene="rbfA"
                     /locus_tag="VFA54_08815"
     CDS             31307..32647
                     /gene="rbfA"
                     /locus_tag="VFA54_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZS50946.1"
                     /translation="MQEKRIERVAEQLRSELAELIENELADPRLGAVTVTSVRLSPDG
                     KQARVNVSPSVQTGGKSEEQSHRRDEEILRTLDHARGFLRHELASRLPLRHIPDLKFS
                     IDHGPQHAQRIQTLLERIKNRAPAAALLLASAILGRQALLGAEMLRYENSTSAMGSVY
                     TIAAYGEDRAQLSAGVGAALQEARRLDDLLSNYKPESELSEINREASRRPVKVSVELF
                     ELLEKCQQYSRLSEGAFDWTVGPLMRIWGFYRGTGHVPEPAKIQEALENVGYRHIHLD
                     PGSRTVSFDKPGVELDPGGIGKGYAVDKMADVLRDAGIHSALISAAGSSIYAIGAPPG
                     EKGWHIRIRDPKSDEITAAEVDLKDQSLSTSGTYEKFFQADGKIYSHIMDPRTGYPAQ
                     GMLSVSVITPKTIDSEAWTKPFFVNGAAWTKAHAPEGFRIFLCEDNGKPCYWIP"
     gene            32753..34942
                     /locus_tag="VFA54_08820"
     CDS             32753..34942
                     /locus_tag="VFA54_08820"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZS50947.1"
                     /translation="MPIRYNDFLRLSRLVRILLATLAAAVLGCSAIFYWIAIRPLAVT
                     SGSISGPRFPDAVAGRDRLDIPHIEAHSISDALWVQGYVTAQDRLWQMDIFRRLSAGE
                     LSEVFGPSMLDRDRESRILGMKRAALAARERLPAEDRALFAAYAAGVNAFIESHRGNL
                     PLEFHLLNYQPKSWEIIDSILVAMHMFRTLTNSWRDEIMKRSLLERATPEQVQALLPV
                     RTGREFAPGSNAWAVSGAHTASGKPLFANDPHLDYSIPCIWYSVHLKAPGLNAIGVTV
                     PGVPGVILGHNERIAWGATNLGFDVQDLYLSPAAERVEQEIIHVKGAADVVLPVSFTA
                     YGPIVAEVGGQKAALHWTAADPAVWQFPFIEIDRAQDWPTFIRALERYGGPAQNWVYA
                     DVDGNIGYQAAGKLPIRKGFDGSVPVPGDGRFDWQGYIPFDQLPRAYNPQSGIIVTAN
                     QNPFPPRYPYTVSGNFASQFRSNRIRELLQNGSRFEPADFLRIETDVYSAFAHEFAEN
                     LVQAINRRKPSEPDLVSVAARLRAWDGQFRADESIPVLVAITYNRFLRSLVEAAAGDR
                     AALYSSQMGTAFVAHVLATRPPGWAKDYDELLVRCLERSIHDLRGMPPRSTATWGQYL
                     ETTLTHPIGRSIPVLRRWFDIGPFPQSGTTTTIKQTTTRMGPSMRMIVDLSNLDNSLH
                     NITTGESGQPFSPHYKDQWRAYYEGRSFPLQFSSPEIEDRLLFHPAP"
     gene            34984..35703
                     /locus_tag="VFA54_08825"
     CDS             34984..35703
                     /locus_tag="VFA54_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZS50948.1"
                     /translation="MNHERWLVSYADFITLLFAFFVVMFASSQVDKRKIAAMAATFDS
                     YAAGGAQASRGNSRPAPSAQAAEAAAPSSQGAAPGLTMAELEPSRDRLAAVLHREITS
                     GEVEISLEPRGLVLSLKDSAFFAPGDDAVSPEARPILAKVAQALRQVPGEIRLEGHTD
                     DRPIHSGRFPSNWQLSTARSIAVLKVLTGELGFPPERLAVAGYGEYQPLESNETESGR
                     RKNRRVDLVVLTQEAASMAPS"
     gene            complement(35727..36683)
                     /locus_tag="VFA54_08830"
     CDS             complement(35727..36683)
                     /locus_tag="VFA54_08830"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HZS50949.1"
                     /translation="MPLQFDDVYLPPLAGISLGFDDATIAGVVGPDGAGKSLLLELAG
                     GAAAAERGRVEAPSQRALIRVGAGGPARIRQEIADATAATTKVLLIDHALALLDGAAQ
                     IHCIQQLHRLRRRGAVILISSHDLCLLERICDVVIALDNGRLVEQGDPGLVLANYRRR
                     ILEHTRLAGALNGMSPSSRRGDGRAQVAAIKIRDESGRPTTTVRSGESITVSAELRFL
                     EPVENPVAGMLIRNRIGICVYGTNTELEQTPIGFRRAGEAVTVEFQFACDLCPQEYTV
                     TVASHDPNGAEHDWLEDALLFSVIDSRYTAGIANLRAKVTVR"
     gene            36865..38163
                     /locus_tag="VFA54_08835"
     CDS             36865..38163
                     /locus_tag="VFA54_08835"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS50950.1"
                     /translation="MAEPEITEQMREDWDRRAREDAAYYVAFGGRRQSPEEFFSTAAP
                     VLQTLRSEFRRFPSAPKTLSALEIGCGPGRLLIPLSRDFGRITGVDVSPEMIRRARRN
                     IESIPNAAAEVVSGSDLSGMRDESFDFCYSYAVFQHIPSRSVVWNYLSEAQRVLKTGG
                     LLKCQFNGLPETERRPADTWSGVRFSAGELRAYCQEHELQLLSLDGANTQDLWICARK
                     QPPGWTRSLAPEDGVHLVEVGNTYTSDRLVPVSGRFSAVSLWVKNLSPGADINNLEIE
                     IGGRKTLPCYVSEDSGRAPVQINAFLPAGLPTGLVQVRLWMLGRPVSNFARLRLIPPP
                     PRRPRLLGVTDAIELLSPFRIETGSIKAEVEEAGLSPEIAADIDGVPAQDIEIRCVNP
                     LSEHYAVIIKTPSQIGPGQHSLNLRLHGRILPAVSIEVAR"
     gene            38163..39785
                     /locus_tag="VFA54_08840"
     CDS             38163..39785
                     /locus_tag="VFA54_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50951.1"
                     /translation="MGNPFFRFPAALAVLLISLVIIAPYTLGRSAPSADAELARWYAR
                     NPKPWQWQTEWGGGSPVLSLGSPGLPLLTAAVASIGLPFERARLIAGGLSYAVAPVGV
                     FYLARALALPAWWTSLAFALAPHRWLDFARQDNAAHALELALLPIILLQFARRRWLAG
                     AGGVLAAALCIPRSWNALPELELAIALFSGLLAKHAKQMLPLAACIAIFAAGLSWEAF
                     FQRSGAVPGTSFSVPAYMASGELGETVLGLPFPDPGWNPVRRQAEQLILSPNRPMDSI
                     LWMQALGIRRLAGPWGAKYDALLACEEAGKAGCLFYATPLRRAAQALLVSRYQWRRLP
                     ALRSLYDRAALNAYVNWSNRPEAAGWRWTRSGTAEIRASLGPDDMILVRQNADGGWTA
                     SADTGPVALFRDPIGYLVLDPGRPGPIFIQLARRTQIRSSFSHPPRRLPARIIPAIDP
                     DGIIDGVAHTPPPVAPGSVISIFGSDLNGAGSRLGSTRVLVSGRPAEVLYAGDNQINA
                     RLPADLPSGRAEIIVETAEGRSDPATLEIQGR"
     gene            39782..41068
                     /locus_tag="VFA54_08845"
     CDS             39782..41068
                     /locus_tag="VFA54_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50952.1"
                     /translation="MSVRCRALLASLWLLLAWPVCGSGLASRLGFYQWDGVGPRSPGA
                     DLLTQAQRYAAETGARVFRLYLGARFDYWHPPGSPLTFADLPEHRGRSREELLSPARI
                     LALPRYRAALHNAAFETIILTTYSVADYGGGPDDLNLFRPWTALEDGIERRQIRELCD
                     LLYDEIGQSAKTVVVANSEADEKMLEIMNYTGSPERAIQNIAAWTRARHEAIEQARAA
                     HPQASLRLVHGFEISLVNLKIAAEAGLFRKSPRGAWSALYNVVPQVPFDLLLYSSYES
                     VNAPFETHNADVNPMQIAVRLRRDLDAIRDHSRASLSVTGKRFFGERFVAVGELGFAR
                     ERFEQLPTGGVLPRLMTAIETAARWGCPYIVIWQVFDARRSGGQPFGFGMIDAGGNAP
                     PLRPAADRCNSVRTCVQRLLEPKRAGPLRRNFPAGQ"
     gene            41196..42611
                     /locus_tag="VFA54_08850"
     CDS             41196..42611
                     /locus_tag="VFA54_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZS50953.1"
                     /translation="MRMLVAGEWIEGSQKIPVQNPFDNSVVDTVPRASQEHIQRALEF
                     AARGAKTMRELPGYERYLRLRRAADLLESRSEEFARTITLEEGKILAEARYEVSRAVG
                     TLEESAEEAKRLSSEVVPLDGAPGAANRFGFTIRIPCGVVVAISPFNFPLNLVAHKVG
                     PALAGGNTVIIKPATDTPLSALKLTEVLLEAGIPPEGIQCLTGSGGEIGDALCSDHRV
                     RKISFTGSRDVGEHICKIAGLKKVTMELGSNSPLIILPDANLEKAAQAIAATGFANAG
                     QVCISTQRVIPLQPIYGELLEAVKPRVEAITTGNPLDAKTRMGPMIREHDARRVEEWI
                     QEAVAAGSRVVAGGGRQGALHYPTIVADVHPRLRISCDELFGPAVAFTPASSIGEAIA
                     LANDTRYGLSAGIFTESLESAMRFAREVDSGNLHINWGPQWRADLMPYGGLKESGFGK
                     EGPHYAIQEMTELKMVVMHLG"
     gene            42684..45110
                     /locus_tag="VFA54_08855"
     CDS             42684..45110
                     /locus_tag="VFA54_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50954.1"
                     /translation="MRRILVAGLLLVTGLWARSAPKRDVLDPLSVFFRHRLHEARRQA
                     QRALSPEQPVEAVLPDAGQIAMIDDSDNVVLAGFDLDQKTVEFKPAAANAAQYTFDVA
                     SSRFDASVLASSTQLSLGDDDSKEVALPFIFPFFGFRYASLFIGSDGHISFGRADDRS
                     WQSGEDPGVRLVAGPPRLAPLYTDLDPSQAGAAISYLSSASRFVISWQNVPVYSPNPG
                     PSVGRQTFQAALYPDGRVEFSYNGIHVAGASAVAVGIAQGGDINLALYTLVDFAAGSA
                     ATFKSTIVEEFINTPQFDIGGVAAKFFRNHEDAYDYLIVLDTVNRGDQIGGACAFSIP
                     VHNWIHGIGLTVSATLPIEEFDLGGTVGASGRFQDLVYLGPLSQYPANPSQMLPSGGI
                     CGPNSTLSVLGQESGHRFLAYLQFIDPATGQPSADLLGRDFQHWSFYFNSDASVMEGN
                     QIIDHGAGASPRFETQKINQHYSALDEYAMGLRAASDVPPSFLVRQPSIDFPPDHPPQ
                     PGILFNGSRQDITLDMIVAAMGRRAPDVTLSQKRFRFAFALLAPAGTTPSQADIAKAD
                     AIRTAWESYFSSITDNRAQADTSLVKQVRLSVWPVSGVIQGHTVSASVALGAPAGAPV
                     TISLNASGPAISIPASVTIPAGAQSASFTIAGNSPGAVEITAQGPDASYETSRAFLLV
                     RPNEAGLTLERVSAALLASGLIAPVTSQTASGGMGPKLDEDLAFRVRDDNYVPYPGLQ
                     LASQASGTGVVVNSSLVTDDLGRVRLTWKLGSTPGMNTLTVMLADQPDVSTQVQAIGV
                     PEPAHLRDLR"
     gene            complement(45192..48752)
                     /locus_tag="VFA54_08860"
     CDS             complement(45192..48752)
                     /locus_tag="VFA54_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS50955.1"
                     /translation="MKQFSYILCWLLGFAAMLPAQTATGRLAGTVIDPAGLPVANAAV
                     GARNEATGASFSSPTNAQGAFTFVEVPPGFYSVEVNATGFRRQVIRRFKVDVAKDNVL
                     PPVRLEIGSVTEVVEVPGGAGQVQTTNAELATTITTQQIQHLPLSDRSPLTLIAIQPG
                     VAYNGNTPTVINGQRTSFSNVTLDGINIQDNYIRDNALDFLPNNLLMDEVSEFTITTQ
                     NGNAALGQGASQVNFVTPSGTNQYHGSAYWFNRNNALAANQWFSNQAGTPKPLLNLNQ
                     FGGTLGGPIRKDKLLFYANYEGYRQRHELLDNTTILTASARNGIFTYRDSRNRLEQVN
                     LLSLAGVSPDPKAAALLQRVPGPANINNFNVGDGLNTAGYQFNVRDNTDRDNVTTRLD
                     YLLSSKHIFSGTYHYNTDVVDRPDVENGFHAVPVIQNHDHVHFLSLAWRWTPLPSLTN
                     EVRGGMNIAPGTFTSTEQTGSQLFSGFIFTNPIVGFQPQGRATNTYNYQDNANWQRGR
                     HNVRFGAQIQHISASPYDKAGLLPAYNIGLSPENPIALDPSQFPGGISTTSLDEATGL
                     LTSLGGIIGSASQTFNVASRTSGFVPGQEYRRHYSLNNYSFYAQDNWKLTRRLTLNLG
                     VRWEYMGRFDERDGLMMGPIFTSAGVRGTLLSDATLDLAGSAVGRPLYAKDLNNFAPV
                     VGIAFDPFGDGKTAIRAGYSINYVNDEIISASENASFQNQGLQSTITNTDLVAAMSGN
                     LPAFPVPAYQMPLLASDNFALNPTSAIFGVNPQLRTPYVQQWNFGIQREVAKNTVVEA
                     RYVGNHGTKLLRGFDYNQVIVKQNGFLDDFIRARNNAFLSQASTGVFDPSYNSRISGS
                     QPLTIFPQLPFGGLLDNGIVQSLIRSGEVGQLAAIYYVNGLTGPIQFVPNPNTFVSDL
                     ITNFSNSSYHALQVEVRRRAANGVEFQANYSFSKVLTDSIGGSQVRFDPFLDLNNGQI
                     ERARAAFDVTHVFNANFVVPLPAGPNHRLHYGPVDRLLSDWTLGVITTWQSGAPFSIL
                     SQRGTLNRTSRSGENTAVTLLNGSQLSQVVHFQMTGNGPYIVNPAALNPADGTGVNSD
                     GAPPFAGQVFFNPQPGQLGTLQRRMFDGPSAFAFDLKLDKQIALTERQRVRLEATFTN
                     LLNHPIFYSGDQSINSVQFGRVTSVLGGARVIQLGARYMF"
     gene            48845..49285
                     /gene="rpiB"
                     /locus_tag="VFA54_08865"
     CDS             48845..49285
                     /gene="rpiB"
                     /locus_tag="VFA54_08865"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZS50956.1"
                     /translation="MRVAVGFDHGGFPIKQDIVNLLKGAGHEVNDKGAYSFDAADDYP
                     DFAIAVARAVAAGEADRGIVVCGSGVGASVAANKVRGVRAGLCHDTYSAHQGVEHDDM
                     NVLCLGARVIGPEVAQELVRAFLGARFSGAERHVRRLKKVLALE"
BASE COUNT         9636 a        15065 c        15096 g         9565 t
ORIGIN      
        1 gaatcgacct gtacagtctc cgctcgcagg ggtggagcgc attcgagttt ctcgattacg
       61 ccgtccggca gcaggtcgaa atagtgcatt tttcggagtc tcgttttctc ggaagcctgg
      121 aagcgagcca cctgcgccaa gtcaaagaat atgccggccg ccttggactc tcgatcgaag
      181 tgggaatggg atcagtgtgc ccgggatcca agaggtttat cgccgccaag ggaacggcgg
      241 aagaacagtt ggcccgcatg ttcgaagcgg cgaagctgct gggctcgccc atcttgcgcg
      301 cctatgtagg gagctgggag gaccggctgg ctggcgagag cggctcgcct cgggaccaaa
      361 gcgcggcgga ccccatcgag cagcgcatcg aagaagccgt gcgagtcctg cggagggtcc
      421 gcagccgggc gcgcgacatg ggcatccgga tcgccatgga aaatcatgcc ggcgacctgc
      481 aagcgcgcga gatgaaaatg ctgatcgagg aggcggggac ggacttcgtc gggtgctgcc
      541 tggattcggg caatcccgtg tgggctgtgg aggatccaca cctcacgctg gagacgctcg
      601 caccttatgt gctgaccacc cacatccggg atagcgctgt gtggcaggaa agcgaaggaa
      661 tcgccgtaca gtggacggcc atgggcgaag gaaccatcgg aatcggccgg tggatcgctc
      721 gcttcgcgga actgtgtccg ggcaagcctt tgtcgctcga aatcatcacc actcaccctc
      781 cccgccttca ccggtgtttc gacgccgcgt tttggaaagc gtttccttcg atgccggcct
      841 gggagtttgc gcggtttgta aagctcgccc gcgagggcaa gccgccgaca agagtactgc
      901 cagcctccgg ctctcccggt tcccaggagc atcagttctg caaggccgcg caggagcgga
      961 tggcgctgga ggacagtctt gcttggtgcc gccggcattt cggagcattc tgccgggact
     1021 gcggaaattc cgacctttcc tgaagccgct tactcgaggg cgaccggacg gtcaaaggtc
     1081 acgtcgagcg ttctcccttg ccggagttcg acttcgcggc cccggctggc gagcacagtg
     1141 gccagtccca cagcgctgcc gacaccggcg ccaatgccgg ctccactcca actccggtcg
     1201 gcaattccgc cgattgaagc gccagtcccg gcaagaatcg cgatgctgcg cgcgtcccgt
     1261 cccgtatccg gcccctgttt gatgaaattc tcctgccggt cgactttctg ctccgattcg
     1321 ttcgaatcca ccgagctcag tcgcggggaa aacttcacca ccctgccgct gggcagcgta
     1381 agtgtttcca aacggatgcc cagttcggcc cgccctgcga ctctcccact gcgcctggtg
     1441 tgcgacacga cgccttgcac gtaagaattc accggcacga tgatgcggcc gcccaccgag
     1501 atggggctgg cggttcgaag atagacgtag tcgccctctt gcgccgttct tgtattcacc
     1561 gagttcacca tgcggagaag cagatgcgct ccctgcggaa tctccggctg ctcgggccca
     1621 ggcgccgcca gtaaaaaact ggaaactgcc agggcggcgg ggagacatac aagcattttc
     1681 atagcggctg gctcctgcct gatagtaacc cctccggcgg cccggagttt cgtgaggccc
     1741 gcaggtgggc gttcgctgga tgacgaggac tgcacgccgg ggcagtcatt gatgccggaa
     1801 cctgcgcggc acccgtgcgg gaggttctcc acttgcgccg agagtcgatg actacggcgt
     1861 ctttccgcgc aagggcggga gcgccgcgtc actgtctcgt gttgaggggc tgggcggctt
     1921 tctcgaaaaa ctctttttgc agccgcgggc tgacctgatc ggccttgatg gccctcagca
     1981 cattggcgat atcggccgcc cacgccttca tatcgaactc tcccagcgcg gtgtttccaa
     2041 gcgaaccgtc gccggcgtag tggtcgggaa acttggcata ccaccagatg ccggtataaa
     2101 cgccagcagg tagatcaaga cgattcaggt cggcgcccga ttcactgcgg gcccgatcca
     2161 tatgcaccag gtccggacgg ctgatcatgg tgtgcgaagt ctccgattcc cctgcatgct
     2221 gatcgacctt cgatttcagc ggcggccggc ccggcgggta ctgcgggcgc gcgtagacgt
     2281 agaccacata gtcgcgcggg cttgacaatt gcgtctgggc aaaatacggc agcagattgt
     2341 tgttgccgcc atggccgttg acgatcagga tttttttaca tccgttgcgc cccatttcat
     2401 cggtggtttc ctggagcagc tccagttgga gcctggggct gtaggccacg gtgcccggct
     2461 catgcctggc ctcaaatatc tggccgaagt aatactcggg aaacacaatc gagtattcct
     2521 gttcggcgcc atgcagcgca gcccaccgga cattgatgac gtcgttgccg atcggcaaat
     2581 gcgggccgtg tttttccaga atcccgatag gcagcacgca ggcgccctgc gatttttgaa
     2641 tcgcctggat gaaatcggcg ccggtgagct cgtcccaccg caccggcagt tgctgggaat
     2701 gcgccagtgc ggcgcaaaac agggttgctc ccaaccaagc cattccgcga gacgtccagc
     2761 gcataagtct ctccctcctt ctacgcaaga ctgggaagat gcaagcaatt ctaacgcaac
     2821 gcggctgggg aatggaaaag cggctcctca ccgcgccttc ggccgaagag gcggcgcccc
     2881 tttcgcaagt acggcctcgc gctcgatcgc ggccgaccgg atagcacgcg cgctggaatt
     2941 gcattacaat ggctcgcagt ccttcgaagc cgcgtgatcc agagcggctg cggagaacgc
     3001 tggcgaagga gcctcaatga atgcgcccgg cattacttct gttcatggcg gccctcgcgt
     3061 ggggccagca agccgagatc cggaatccgc ataccacacc ggctgacgtg gcggcaggcg
     3121 ccaggatttt tcactcccat tgcgccgagt gtcacggagc gagaggcgaa ggaggccgcg
     3181 gccccaacct aaccacgggc gtattctttc acgggagttc cgacgccgat ctgttcaaca
     3241 acatttccaa cggcattgcc ggaacggcga tgcccagctt gtttttcccg cccgatggcg
     3301 tctggcaggt ggtggcttac gtgcggtccc tcgcccaagg aggcggtgag cagcgtcctt
     3361 ccggcgacgc ggctcgcggc gccatgctgt tccgcgaaaa gggctgcctg ggctgccacc
     3421 tagtccgcgg agagggcggg atcaagggtc cggatttatc ggtggtcggc tcacagcgta
     3481 atctcgagca tttgcgccag gcgatcgttg accccaacgc cgaggtgccg cgcgagtact
     3541 gggtcgccaa aatcacgctc gaaaacggat cgagctacac cggttttgtg atgaacgaag
     3601 atacccacac cgtgcagatc ctcgacttca cccaagggct caagtcgctt tccaaacaga
     3661 gcttccgcaa gttcgacatc gacaagtcgt ccctcatgcc gtcctataaa ggacagttga
     3721 acgaccgcga aatcaacgat ctggtggcgt acctgtggtc cttgaagcgc gagtcgcacg
     3781 cccacgaagg gaggtcggaa tgaaaggctt acgcctggcc ttgtgcgccc tgctggcggc
     3841 gggctcggtc tacggacaac tgacttttga gcggctcctc aaggccgact ccgagccgca
     3901 aaactggctg acctactccg gctcttatcg cggctggcgg tacagcaagc tggaccagat
     3961 caaccggcag aacgtcaaga atttgaaagt agcatgggta tatcaaatgc ctacaaccca
     4021 ccggatcgag gcgactccgc tggtcgtgga cggcgtcatg tacgtgagcg agcctccttc
     4081 caatgtcgtc gcgctggacc ccgccaccgg ccgtcagttc tggcgctaca ggcggagcct
     4141 gccgagcaag gtgaatgtct gctgcgatca agtgaaccgc ggcgtagcgg tgctcgggga
     4201 cagggtgttc gtcggcacgg tggacgcgca cctggtggcg ctgagcgcca ggacaggcgc
     4261 cgtgctctgg gacgccgaag tggccgatta tcgctccggc tacagcatca ccgtggctcc
     4321 gctcgtggtg aaggacatgg tcattaccgg aatcgccggc ggggaattcg ggattcgcgg
     4381 ttttctcgat gcttacgacg cacgcaccgg ccagcgccgg tggcggttct ggaccattcc
     4441 gggacccggg gagaaaggaa atgagacctg gagcggcgac aactggaagc atggcggcgg
     4501 cccgacttgg gtgaccggcg cctacgattc cgctcagaac ctgattatct ggggcaccgg
     4561 aaatccctcg cctgactgga atggggacgt tcgccgcggc gacaacctgt atactgattg
     4621 cgcgattgcg gtggacgccg acaccggaac gctgagatgg tatcaccagt tcgtacccca
     4681 cgacgtgcat gactgggacg ccgttcaggt gcccgtgctg gtcgacagcg agtggcgcgg
     4741 aaggcagcgg aagctggtgt attgggcgca ccggagcggc ctgtacgagg tgctcgaccg
     4801 ggaaaccggc gagtttttgt ttggcaagcc gttcgccaaa gtaacctgga tgagcggttt
     4861 cgatgagaaa ggccgcccca ttcgcaaccc cggagtggac cccaccgagg aaggagtcta
     4921 cgtatggccc ggcgtgcagg gcgccaccaa ctggtattca ccatcttaca gcccccagac
     4981 cggcttgttc tatctcacgg tctgggaaaa caagggcttc tatcaaaagg gcgaggccga
     5041 ttataagcct ggcaatctgt ttatcggcag cgtgcccaaa atcgacgtcg aggaggatcc
     5101 cggctacggc gcgattcggg cgctcaaccc gaaaactggc gacaaggtct gggaatacaa
     5161 ggtgcacacc aagccttggt cgggcgtcct ctcgacggcc ggaaaactcg ttttcggcgc
     5221 gagcggcggc tggatcaacc gcgacgagcg cgaggaatcg gagagcagct tttatgcgct
     5281 cgacgccgag tccggcaaag aactctggcg gatcaatctc ggcgggaaca tgtccgccaa
     5341 tcccatcacc tattcagtaa atggcaagca gatggtgacg atggccgcag gcagcggcat
     5401 ttttacgttt gcgctgccgt aaagtccggc ccgcccgaga ccttggccgc tctttccatt
     5461 cttggcctcc tgaaagtacc caggccgccc agggtgcccc gctcgggcga atccggttca
     5521 cgctgagagc ctccgaaacc agggcgttcg ggatctttcc agcgggatcg gcaggccgcc
     5581 gatggtccgg gccagcgacg agatagcccg cccaaaccgg gataactcct cgatcggccc
     5641 tggacttcgc accacctgct gcgccgcggg gtaatcattc ggcaccacaa gggccgcctg
     5701 gcggccgagc agttgctcga tctgttccgc ggcgccgtta tcgcttgcct ggcgattcag
     5761 caccagggcg agctggtgcg ccttggcgcc ccgcgccgcc aactcgtaga ttcgcctgcg
     5821 ggctagcagc acggaggcct tttccggcgt gcagatgaca tggactctcc ggcactcggt
     5881 gaccatcgcc tctgtggcgt cgttcaccac ctcgggcaaa tccaccagaa cggcgtcata
     5941 ccgggttcgc acataggtaa gcagccgccg gcagctccac tgcatcaggt gccccgtttg
     6001 gattgcaggc ggcgcgggga gcaggtcgaa tccgtccgtt ttggtcacca gccgctgcca
     6061 gcttgtgtca tccagccgat gacaattctc caaagcgtca acaattgact gctcgggctt
     6121 caggtccagc aagaaagaga tcgggccgga gtgaacatcg gcttccacga gcagcacccg
     6181 cttttgacag cgctgggcca gatagcggga aacgcataac gccagcgtgg tcgctccact
     6241 gccagctttg gccggaacaa acgcaaatat attgtcggga atcgccgctt tttgaaattc
     6301 ggccgctgca cggagggcgc gctggaacgc ttcgggcgag aacggggccg ccaggtactg
     6361 atgcactccc gccgggagcg gcagatcgct gggctctcca aatccgatgg tcgcaatctt
     6421 ggggtaacag gtctggattt cccgaagcag ttccaactcc ctgcgggaat acgtcacctc
     6481 caagaacacg atctccggcg agaaaaccga gagcaactgg aagagctccg aaggagccgg
     6541 gtgccgggag agagttttgt agatgccaat gccgctcgcg gcgcagcatt cctgcatccc
     6601 ggtgagaaac cattccgtca cgccgataat gaacgctgat tgcatgcagg acctgagcag
     6661 cgaacgcgat ccccggagcg tgacaagatg cgcatccgcc gctttctcct ttcgacgctc
     6721 ttgcaggcgg caacgagagc gcacggaaag gccgcccaga ttccctgccc tcctgctgga
     6781 gctgtctatc tgcgctccct cgccgcaatc gattgccaca cggcgaatca agcaccaacg
     6841 atggtatggc aagactgccg tgcggccaat gggccacgcg tactctccgc ctctcgggcc
     6901 cccgccttag gcgcacggca gtgggaaagg gtacgaaagt cctgttgacg cctgcgtctc
     6961 agtagtagca tgctcagggt gatcgggacc gacgccggca gaggccgaaa ttgcaatctc
     7021 tcaaaattca tcgcgcactg gcatcgcggc cggcagggtc tccggcggga tctcctgcat
     7081 gattaccgtt ctaatcatcg gaacttccgc atccctgttc atctgttggc tccgctgcac
     7141 ggttcttctc attctcaggt cgatgccggc tcgcagctgc gcccacgaag cggccgaggc
     7201 aaacggcatg aacttcctcg acctgcggcg gcgattgtac agtcttgtat cagaggaaag
     7261 cctgcccatg ctgtgcgaga tgctaaagaa agacttaaag ctcaccaaga ttctgttgca
     7321 acaaatgggc ccggaaactg gcggccgcat ctccagcgac ttcctcttga cgctgaactt
     7381 tcgattactc ggattgcgat tcgccgtctt gcgccgcttt cgcccgggca gcgcccgatc
     7441 ggctctggcg gaaatgtcgg ccacgctgga atatttttcg aattgcctaa gccggcgcct
     7501 ggccctggca tcccaaccgg cccgtttctg aacccggtat tggcgatgag cgctcgattc
     7561 ttctgaattc aattcggctg cggaaacgcg gcacggcagg cgaaacttac gcgcgcgaaa
     7621 gcccgcgaaa ccaaagctcg cagccatgcg attcggggcg cttctgagga ttaaggcctg
     7681 ctccatcgcc gcgccacagt ctcgccagag cggcggcttt cagccccgcc actgccagtc
     7741 gcggatctcc ggcaagtcgt cgccgtgctc ggcgacgtag agtttgtgtt cgatgagctt
     7801 gttgcggaga aactgctttt cgtgggcggc gatcgattgc agtttcggaa cgcggtctat
     7861 tacgtccatc gccagatgaa accggtccaa atcattcaga acggtcatat caaacggcgt
     7921 ggttgttgta ccttcttcct tgtacccgcg cacgtgcatg ttgtcgtgat tgtggcgacg
     7981 gtaggcgagg cgatggatca gccacggata gccgtggaaa gcgaagatca cgggcttatc
     8041 cgtggtaaac agcgaatcga aatcctgatc ggatagccca tgcgggtgct cggtcgaagg
     8101 ctgtaaggtc atcagatcga ccacgttgac cacgcgtatt ttcagctggg gcatgcgctg
     8161 gcggaaaaaa tcgacggcgg ccagcgtttc catcgtggga atgtcgcccg cgcaggccat
     8221 caccacgtcc ggttcaccgc cctgatcgtt gctggcccaa tcccagattc cgatgcccgc
     8281 cgtacaatgg cgaatcgctt cgtccatatt gagccattgc caggccttct gcttgcccgc
     8341 gatgatcaca ttcacgtaat ggcggctgcg caggcaatga tccgccaccg acagcagcga
     8401 attggcgtca ggcggcaggt agacccggat gatgtcagcc tttttattca ccaccagatc
     8461 gatgaagccc gggtcctggt ggctgaatcc gttgtgatcc tgacgccaca cgtgagaagt
     8521 cagcaggtaa ttcagcgacg cgatcggccg gcgccaggga atctggcgcg tgactttgag
     8581 ccatttcgcg tgttggttga acatggagtc tacgatgtgg atgaatcctt cgtagcagga
     8641 gaagaatccg tggcgtccgg tcagaagata tccttccagc cacccctggc acatgtgctc
     8701 gctgagcacc tccatgaccc tcccgtcggg cgacacatga tcgtcggaag gcagaatctc
     8761 ggcggttgaa acgcggctgg tcgcctcaaa gaccgcgttc agccggttgg aagccgtctc
     8821 atccggtccc atgatacgga aattccgggt ttccccattc agcttcatca cgtcgcgcaa
     8881 catgcggccg agcacgcgcg tcgcctccgc agccaccgct ccgggcttcg gaacgtccac
     8941 cgcgtaaaac cggaagtccg gcatgcggag atccttcaac agcaggccgc cgttggcgtg
     9001 aggattggct cccattctgc gctgcccttt cggcgcaagc tcggccagct ccggaattaa
     9061 ttttcccttt tcgtcgaata gctcctcggg ccgatagctt ttcatccagc tctcgaggat
     9121 tttgaggtgg tccggcttag ccctgaagtc atcgagcggg acttgatgag cgcggaacgt
     9181 gccctcaatg ggaacgccat ccacttcctt cgggccggtc cagcctttgg ggcttcgcag
     9241 cacgatcatc ggccactgcg ggcggcgcgt aaagcctttg gtgcgcgcct cgccctggat
     9301 cagttggatc tcgagcagga cggtctccag agcggcggcc atcgcctggt gcatcggctc
     9361 ggggtcttgt ccttcgacaa agtatggctt gtacccatgg ccggccaaaa gctgttccag
     9421 ctcttccctg ctgatgcggg ccagtacggt cgggccggcg attttgtatc cattcaaatg
     9481 aagaatcgga atgacggcgc cgtcgcgcgc cgggttgaga aacttgttgg agtgccagct
     9541 tgccgccagc ggccccgttt cggcctcgcc gtcgccgatg atgcagcagg ccagcagatc
     9601 gggattgtcg agtacagccc cgaacgcgtg cagcagggaa tagcccagct cgccgccttc
     9661 gtggatcgag cccggcgtct ccggagccac gtggctcggc acgcccccgg ggaaagaaaa
     9721 ctgcttgaaa agccgcttca ttccttccgc gttctggccg atgttcgggt acacttcgct
     9781 gtaactacct tctagatagg tattcgcaat aatacctggg ccgccgtgcc ccggcccgat
     9841 tacgtaaatc atgttcaggt catatttctt gatcaggcgg ttgcagtgaa cataaatgaa
     9901 gttcaggccc ggcgtcgttc cccaatgccc cagaagccga ggcttgatgt gctccagttt
     9961 caaaggctga gtgagcagcg ggttatcgta caggtagatc tgtccgaccg aaaggtagtt
    10021 ggcggcgcgc caataggcgt ccatcctgcg aagctcgtcc cgggtcagcg cgcccctctg
    10081 cgccgctccg ggttccgctt gatctcgaac gggtgttccc ataggaagcc tccttcacta
    10141 gccggctctc ttacagaatg ccagaaggcc aagacggcca acacggaaag aatagtggaa
    10201 acgtcaaagc aggtatggcg ctccacgccg cgcattccac tcttgtgtgg gacgccgcgc
    10261 cgcgctggcg atcagcctgt gcggcgtaaa cgcgctcacg gaccgcgcct cggcctccgc
    10321 cgcagagtat gaaaagcagc catggcatcg gaactgctgc tccgatgaac ggaggtccaa
    10381 catggcgaca gcaatgccgg gtcccgaaca tcgagagggc cgggtggccc gcgcgatcga
    10441 gcagcgaaca gcgaaattac cttccgatgt cttcctctgg gcggctctgg gttccatggc
    10501 cgcttccctg gttctgcaac tgatgggcga gcggcaaaga tcaaacttcg tggggcagtg
    10561 ggcgccctct ttcctgatac tgggtttata caacaaactc gtgaagctgc acggttccga
    10621 acgagtgtga atttttgacg ggaaggtccc gaaatcggcg ggacgaaacc gcacagagat
    10681 gcgcaacccg gccgttcggc cgccgagaaa gcgaccggcc acaggcccaa aaaaggaaac
    10741 ttgatgccta tcgagactga aaagtgcgcg cacccgccgt gccgctgcgc ggtttctact
    10801 ggcgagaaat attgcagcca gtactgcgaa gacgccggcg aggacgaaac ggaaatctcc
    10861 tgcgattgcc gccaccctgg ctgctctgcc gccgaataaa cgcccagctc ttggggcaga
    10921 gctttacggc tgcggccgcc cgaagcgaag tgggcttgtg tcgcgcgcga gccttgcaag
    10981 ccgcacgcgc gcgaatcacg tttccggagg ggtctggaaa gcggacgcgt tctggacgcc
    11041 catagcgagc tccggcagaa ttcccaactg gacatagatg ggacgcacaa gatcccgaat
    11101 ggcggcaagc tgccgaaaaa accagaaact gccggccaga caaagcatgc ctcccagaat
    11161 aagcgtctgg ggcgctccga tgcgggccgc cagacctccc gcgagcaggc tgccgaaagg
    11221 cgtcatgccc ataaaggcca tcgagtagta ggccatgacg cggccgcgct tttcatcgtc
    11281 gacgatggtt tgcaggatgg tattgctgga ggccatttgc tgcatcatcc cgaatccggt
    11341 gagcggcagg aggagcagag agagccagag cacgtgagat agcgcaaagc cgatcagcga
    11401 agcgccaaac agagccgagg caagggcgat gcgccggccc aggcccaaca ccgtgcggcg
    11461 catggccagg ccgatggcgc cggccagtgc tcccaagccc gaggcggcca tcagaaatcc
    11521 caaagtgtgc gggcctccgt ggagcacact cgacgcgaaa atcggcatga ggacgctata
    11581 gggcatgccc accagactca ctaaagccaa aagcaataag atcgagcgga tcggcaccga
    11641 ctgaatcaca tagcgccacc cttcgtgcaa ttcgtgaagc acgcgccgct gcggccggcg
    11701 ggttagagcc ggggccatcg acatctctag cagcgaaacg atcacggcaa tatagctggc
    11761 gccatcgagc agaaaacagt aaccctcgcc tacagccgcg atgatcacgc cggcgatcgc
    11821 cggccccgcc agccgcgccg cgttgaccat ggacgaattc aatgcgatgg cgttgcccag
    11881 atcttcccgg ctttcgacca tttcaattac aaacgcctgg cgcgccggca tatcgaacgc
    11941 attgatcaaa ccttgcgcca gggaaagcca aaggatgtgc gaaaccgcga tgacgccgct
    12001 gagggccaga gccgctaaag cgaaggattg ggccatggaa agaacttgcg tggcaaccag
    12061 ggtccggtga cgatcccagc ggtccaccca gacgcccgcc agcggaccca agaagaaggc
    12121 ggggatctgc ccggcgaaac tgaccagtcc gagcagggtc gcggaatcgg taagccggta
    12181 aacaagccag cttgtcgcca cgcgcgtcat ccaggttccg atgagcgaga tgctctgccc
    12241 gccgaagaac agccggtaat tccgcgatcg cagcgcccgc agcgcgaaag tccatccagc
    12301 cacagcttct attgtcctgt aaacaagcga actgcgctga atgtcggcgg cactgaccca
    12361 gccggcatgc gccggcgaca tcacagccgc gaggatttgt tttagcagaa ccgcgccgag
    12421 gccgcgagcg cggcacgctc aacttaaggc ggtcaggacc tgcacacaaa tgcgttagcg
    12481 cccgccggca tggccccgtc gcgcgggcta gaaattcgcg acgttcagat tgtaaaactc
    12541 cgcctccaga tactccagag ccagggcgaa atttagaatc gcgacgtcgt tcgcagccga
    12601 cgcggttccc gcataatcca gcggtggacc gccattcaag cttgcgatga acggcgaagc
    12661 cagtgtagcc acctgggcgg gagtcagtgg cgtctcaaaa ctctgctcca tgccgtatac
    12721 gttcgcggta ataggcgctt gcagcagtga attgagccag cccgcgtggc gggcttcgat
    12781 gagcgcaatc gagccggccg ctgccaggta tccgcggttg aagataatcg gggcggcgcc
    12841 gaggtatgcg ccaacgccgg tgttttcaag agcctgtgaa acctgggcaa attgccggaa
    12901 attgggttga accaggttct gaaacgtagg tttcggacgc gcgcttgctc ccagcgcagt
    12961 caaaagggct gccacgtgag cattttcgtg cctttgtata tccagaaacg tgttccgcgt
    13021 ctccctcttg tcttcgccgt tgttgacgtg gtctgcttga gcgtttcggg ccgtcaaaaa
    13081 agccactccg ccggcgccag ccaggacagc ccggcggatg aatcgccgcc ggccagcgac
    13141 tgcttcctca acgtcttgag tcaagcgatt ttgggttatc ttcgctgagt catttgaaaa
    13201 atgttgcgcc attatcgtcg tacctccgta catttgtcga gcaagtctaa gcccacgatg
    13261 cgggctggcc ttccggcttc ggcctcgaaa agcggatggc tttttcgaga agagcgttcg
    13321 cacacaacgt cgccgtctct atcgccacaa aggcccgggt gacaccgaag ctcgtcccga
    13381 atcgactcat cctaaatacg aagagaaaag ccgaatcgga tttcgcagcg tgccgaatcg
    13441 ggggttgtat ccgacccacc gtcagtcaaa gccgcctgga actagacgaa tagctaggaa
    13501 aaaagatcga gacaaattga attctctggc cgccttcgca aatcggctgg gttgggcttt
    13561 ccataatccg tggcggcgct ctggatcctt tctcgagccg ccacgccctc cttgttgggt
    13621 ggcgtcccca gcgggatttg aacccgcgtt accgccgtga aagggcgatg tcctaggccg
    13681 ggctagacga tggggacgat tacttggcgg gaccgcgatc tctaaccaga aattcagtct
    13741 agcctgagca tccctgtgcc gtcaatccaa gagcgttccg gtgtattatc gagaattcgt
    13801 cgggcggccc gcccagttgg cacgcggttt ggccgagatg ctaggctatg cggcgtattc
    13861 cgttttggtc aatcttattc gggcttctgg ttgcgagctc tctccgggcc gtggagatcg
    13921 ccaccgggat gatgctcggc ttgcgttacg accccgacgc tttttacttc cgcacagcgg
    13981 agaatcctgc ctggcgcaag acgtataccg gcaagggcta ccgcggagag gcgcgcggca
    14041 agctcatgaa tgtgcgcctc gcccaggcgt tgtttgaaga cgagtggctg agcgaacgcc
    14101 ccttcgacgc cgcggccaac actgcccgcg tgatcgacgc cctggacttc tacaagcgcc
    14161 acggtgtgct ggcgatcagc gtcggcctcc agggagcgga tcccggctat gcccccaacg
    14221 tcaacggcat cacgcggcaa agcgggccgc agtacgggcg cagcggcggc actctcgtca
    14281 gcgcgttccg tcccgatggc tcactcaagc cggcatggct cgcgcggctc gagagccttc
    14341 tccgcgccgc cgatcagcgc ggcatgttcg tgtgcctgat ctatttctat ccgggactcg
    14401 acgtcgtgtt cgattccccg gagacgattg aggctggcgc gcgccagatg accgactggc
    14461 tcattgatca caaattccga aatgtaatca tcgacgttgc cgacgaatgg gacgccccgg
    14521 gccagaagtg ggaccaaagc gattacattc cgcaaaacgt gggtggtatg atcgagcgca
    14581 ttcgtgaacg cttccaggcc aagcgagccg actttacgct gccgattgga gcctccgcca
    14641 gcggacgaat gctttacccg gcctctttgg ctgaaatcag cgacgtcgtt ctgctccacg
    14701 gaaacgggcg cgcgccggcc gaaaaagccg accgcgccca gcagtacaaa gcttaccagc
    14761 gcccgttgtt gatgaccgga gacgacaacg gcagagaaac caccctttcc catctcgccc
    14821 gagagcaggc cagttgcgag gcgttttttc gccatggggc aggctggggc tacactccct
    14881 gggttcaggc cgaacgtttt cctttctatt acctgcccgg cccgagcccc gatttcaccg
    14941 accaaacgcc cgtggcagaa cgagaccgcg cctatttcca cgccgtattg gatgcgatgg
    15001 cgcgcttcgt cctcgaaaag cctcccgagg gaaagaagtg gaagagcaag ccgtgagccg
    15061 gcatgcctga tgcgcactcg cctgcgccgg agctcaagcg cgccggcgaa tcagcgctca
    15121 gccgaaggga agtagcggaa actttgccct ggcggcggac gttatatcat aagaggaacc
    15181 tggctggatg aggagatacc tatgaagaca ctttattgtg ccgctatgct ggcactggta
    15241 acggggacaa gctggttgat cgccgatacc gtgatcctgc gggacggccg gcgcgtggag
    15301 ggcacattcg tgggaggcga ctcgcgccag gtccgcgttc tggggaccga cggcaatctg
    15361 caaactctgg atattaatga tgtcgagagc atccggtttt cgggcaatgc ggccgcgtcg
    15421 gccgctcccc cggccagcgc gccccggccg gcttccagcc ggcccgctct ggtccgtgaa
    15481 cgctcggaag ccgtcgcttc gcaacagccg agcgtcgtgg tccctgccgg gacggtgatc
    15541 acggtgcgca tgatcgattc catcgactca gacgtaaacg gggccggcga gcgttttcgg
    15601 gccagcctgg atgatcccct tcaggtcggc gagaagatca tagcgccgcg aggttcggat
    15661 gtgactgtgc aagtcgtgcg cgtgcagcaa tccggaaaat tgaccggccg tgacgaagta
    15721 gctgtggatc tctacgacat cagcattaac ggcaggaagt atcaggtggc gtccagttcg
    15781 gccgaggtct caagcggctc gcgcggcgcc cgtactgcaa aagtcgcagg cggcactgcg
    15841 gccctgggcg ctataatcgg cgcaatcgct ggcgggggca agggagcggc catcggagcc
    15901 ggcgcaggcg ctgccaccgg agtgggcatc gaagaagtga cgcacgggca gcgagtcaaa
    15961 atccccgcgg aaacccgcct cgaattcact ctgaagcagc cgctggcggt ttactgagat
    16021 tggctggagg caagcacggc gtttgatctt cgtccctccg gccaccatcc cgcgccgccg
    16081 gcgcccacgc cgtttgattc tacgcagcgc agaatctgct tgtgggcgtt tttcctgaaa
    16141 tggcaacggc aggtccctcg ctgcggccgg aatgccgcta tcgggagtgg gcgaacggtt
    16201 cagtaacgtc cgccgaagag cagatttcga catccggcag catgactagc ccgggggtgc
    16261 atcttccacc cctccggctg gggatactga gagagctttg tcggaaagcg ccctcctgcc
    16321 gccaatcgcc cgattctgct gcgcggtctt gtctcggcgc ttacccgaag aactccggct
    16381 cgcagcactc tttttctaaa acagcactcc gagcagccat tcccactgat cgggctcgaa
    16441 gcgcagcaga ccgccgcgtt tcgcaatatt cgccgtccaa cgcgtgtagc gcagttcagg
    16501 gagcagccgc agccggcccc gacgaagctc cacaccgctg ccggcggata cgcccgggta
    16561 aaatcgtttg cgcaaatcga acggatctgc ggtatcgatt ggagtgcggg ttgtagttcc
    16621 ggcgaccagg tcttgcgccg tctcttctcc gcgagcctgg acggggccga catagcgcac
    16681 cacgccgcct ccgctcgcgt aaggatggag tttccggcga aagcggtatt tcagcaagat
    16741 cgggaagtcc caggaatcgc ctttgatatc aaaagcggca gtcgtcgata ttccccttga
    16801 actgtctata gtatgaatga tacctacgta ccccatgcgc ttgtaaagca cgtcggcctc
    16861 gatccccagt acggtctcgg agcgccactc agccgaaacg ccggctgtgt agcggcgcgt
    16921 cgccgccgag tagtcagcgg aactgctgag gctcgcggcg gagcgggtgt caaaatactg
    16981 tgtcatcggt atgccggcct tgaacccgag gagaagactc tgggcgaatt gtggagcggt
    17041 tgccgccaaa ccccaaagga gcaggcgaag caggaaaggc acggcagacc tccttttctc
    17101 ccccgccaag atggatagcg gatccgtccg gcgctgcgcc ggccgatggg cccgctattt
    17161 cgtaggctgc gtcagatcac acgcagccaa actcatgagc cggccatcat aaagattccg
    17221 tagccggcgg cggagaaatt cccggccgga aggcatagcc gcctcaccgc cttgccgcgc
    17281 gcctttttcc tgtcggcgtc aactccggag gggcaatcca tgccagcaaa tcagcttccc
    17341 cagccgtagg gcgaagatcg gcgactgaac cgcctgcttg ggcgctgtcc cggacccaat
    17401 cggacgggaa gtcctggctc gcgttgtaca acagcagctt gttttccgta tcgagcaccg
    17461 ctgcggcccg gaagtcgccg gctttgcgca gccccgggat gaaaaacttg ttgatatatt
    17521 cttcatccga gtccgcgccg aattgcgcga gatcggcggc cagattgacg ccggctcccg
    17581 ccatcgagcg agcgaagtag ctccatactc cgcccatctg catgcccacc aggtttacgg
    17641 tctgggcatt ggaacggcct tgcagatacg cgagggcggt caggatgtcc tgcacgcggt
    17701 tggcgtcatc ggtttggttg aatacggtga acgcgcgctt gctgcggtcg cgcggcgcgc
    17761 gggcgctgcc ggtctggaag gcgtcaatac ccatcaccac gccgccgcgg tcgagaatgc
    17821 ctttcaccaa cccattgatc gtctgagaag acgagaggac ccaagcgacg ccctccgggt
    17881 gaacgatcag cgtgggcggc acggccggat tgggtctgac gggcgtcagc cagacggccg
    17941 ggacgcgatc gcccttgccg gcgcggccga caaacagggt ctctccgtga gccaaagcct
    18001 ctttcttctc agaaatcacc tccgcagcgg ctggtttcgc ggccaggagc gagaaagtca
    18061 agcgctcgtg aaacgcctct ctggctttct ccagagcggc gcgatcatgc ggatgcagtt
    18121 cgtcgatccc tcgtttcgct tcggcgattc gctccgagat aaattgctcg agggaagtga
    18181 tcgcacccgc cgggcgctgg cgcataaaga gcgcgagcag attttggggc tgctcgacct
    18241 gaaaccgctt ctccgtcacc ggacctttcg ttcccagcag ccgcgctccc aggaacgtgt
    18301 acacagcctc ccggctctcc ttgttgtagt tgtggacctg gtcgatctgg atggtttcga
    18361 cattttgctc cgcgcccaaa agcctgtaga tgctctgcac ggcgggaaat tcctcatgag
    18421 gcgtattgcg ggtccaatcg ccgctggccg aaaccatgag cagcggccgc ggagccatca
    18481 gcgccgcaat gaccatattg ctcgtatcgt tgttgccgcc gacgcgcaga ttcggagcgt
    18541 tttcgcaaac gccgccctgc atgctggccg atatcatatt caccggcgca gcagccttga
    18601 tgcggtcgtc aaccgccatc aacagaaaag tttgcgttcc gcctccggaa gcccccgtgg
    18661 cggcgatgcg gttggggtcg acatccggca gcgagctgac gaaatcgacc gcgcggatgc
    18721 tatcccaaag ctgcatgccc atggggccga tgctccaaag ctcgtcgcgc ttgcttccga
    18781 attcgtgcgg cacctggttg gtatcgttgt agccgatcat gtcgtagctg aaaaccacga
    18841 agccttgcag cgccaggttg atgcaccgcc ccggtacgga cacgagctgc tggttttcga
    18901 gccggccgta agcccagtgg ccgtgcgggg agaccacgcc gggaaacggc ccctgcttcc
    18961 ctaacggccg gtagaggttg cccccgagat agaacccggg ataagtttcg agcagcacct
    19021 tctcgatcga atagccgttc ttttcgagct tgccgaacac ctcggcgtgg ataggcccct
    19081 tctgcggcat cggcagcagg cccgcgctgg ccagaatctg cttccgcaag aacgcggctc
    19141 gctccagcca cgcctcgcgg ctggtaaaga ccggcatctg gaagtgggtg ttggagtcgt
    19201 gaatgaccgt gttgcgtgaa tcctgctcgg gaatctgcgc ggctgcggag ctgaaagaaa
    19261 gcagcaagaa tagagagatg gctttcgtca tagccctaaa tctatcacaa gcaagtggaa
    19321 cccgcccaag atacgggccg cccctgccgg cctgctgcgg gcgagtgaag agccgcggcc
    19381 gagcgccgga gccgcgcaaa cgaaaacgca aatgataata tgggtgggag ccgaactcta
    19441 cctgaggcgg aggacccaaa tacaggggcg aagtccgatg tttatcgaga cggggtactt
    19501 tcaagcccta gcccgtcagc taacttcgca ggctgatttg aaagccaccc tgggtttcag
    19561 ggtggtccgg agctggtctt aatgccggtt caaaacatgt ccaaggcgac caaggtagtt
    19621 gtcgccacca cagtcatgtt gtccttcatc tcgttttgga gggcagccgc gattgtgctt
    19681 tgcgatctcg ggtcgtcggc tttctatgcc ggaggcctcg cagaaggcta cgtgggcaag
    19741 tcggccccgt ggttcattct gggaatcatg ctgttctcca acgccgtcct cgcggtttac
    19801 atcgagagtt gcagcatgtt cgtgcggggc ggggtttacc gggtcgtgca cgaggcttta
    19861 ggaggcacgc tcgccaagtt ttccgtttcc gccctgatgt tcgattacgt gctcaccggt
    19921 cccatcagcg gcgtcagcgc cggcctctac ctggcgggac tttggaacga gactctggac
    19981 ctcatccacc aaaaagcctt ccaggtaaat acccagcatt tcgccgccgt ttttaccatt
    20041 ctcgtaatcc tgtatttctg gcgaaagaac atcatcggaa tgcatgaatc cagccagaaa
    20101 gcgctgcgca tcatgcagct cacgaccgtg atggtcgtga ttctgatcgg ctggtgcctc
    20161 ctcaccatcg tcaagcgcgg ctacaatcca gtcccgctgc cgaccctcga gaatattcac
    20221 ttcagcgatg aagccctggg ctggctgaag gggacgatat ttccccatat aacgccgatc
    20281 gccattctga tcgggctagg ccactcgctg ctcgccatga gcggcgagga aagcctggcg
    20341 caggtaaacc gggaaatcgc ctcgcccaaa ttgaaaaacc tcgagcgcgc cgcctttatc
    20401 attttcatct atagcctggc gttcacttcg ctcgtatcgt tttttggggt gatgatcatt
    20461 cccgacaacg tgcgccccgg ctatcttgac aatctggttg gcgggctggc gatgtcggtg
    20521 atcggcccgc tgccattgcg gctcctgttt cacggctttg tggttctggt gggcacagtc
    20581 attctggccg gcgccgtgaa tacggctctg gtagggtcga acggcgtgct caatcgcgtc
    20641 gccgaagacg gcgtattgcc cgattggttc cggcagccgc acagcaaata cggcacgaca
    20701 aaccggctga tctcgttagt ggcgttcctg caaatcttca ccatcgttct cacgcgcggc
    20761 gatattacgc tgttgggcga cgcctacgcc ttcggagtag tttggagctt cgccatgaaa
    20821 gctctctccg tggtggtgct tcgttacaag cagccgcaag gccgggaatg gaaagtgccg
    20881 ctgaacttcc atttccgcgg cgtcgaaatt cccgtggggc ttggcttgat tacgctggtc
    20941 ctgttcgccc tggcgttcac caacctgttc acgaagaaag tggccacgat ctggggcttc
    21001 ggcttcaccg tggtcatttt tgcggtattc gaaatcacgg atcattataa gcgccgcacg
    21061 gaggcggcgc acaccgagga actcgaaaaa ttccgcctgg aggctcccgg cgacctggat
    21121 gtaacgtcga tcacggttcg ccccggaaac gtgctcgtcg ccgtgcgcaa ccccaaccaa
    21181 ctcgagcatc tgaagaaggt tctggacaag actgacaccc ggaaaatcga tatcgtcgtg
    21241 gttaccatca agaacgtcat ccgggccggc tctggcgagc acgatttgac caccgaacag
    21301 gtgttcacta cagacatcgc ccagctcttc agcaaggtgg tgtctctggc cgaaaaggcc
    21361 ggcaagcacg tcgaactgat ggtgattcct ggctccaatc caaacgaggc gattgtcgag
    21421 acagcccaga ggctgcgctc gtcattgatc gtcctcggct cttcgggcaa gatgagcccc
    21481 caggagcagg ccaaggcgtt cggcgatgcc tgggaaaagc tgcctcggcc tcgcccccag
    21541 ttgtccttgg aaatttacga cgccgcgaag ggagaatctg tctatttcaa cctggggccg
    21601 cacccgcccc gattgtggcc ggaggacgta gatttgttgc attctctgtg gctggaactc
    21661 accgagaaag gctttggcgc caacctgcac caccgggacg tggtccgggt tgccctgcgg
    21721 cgcctggacg agcaattgca ctcggaccat agcaatgagg tattggagga cgtccgccgc
    21781 gaagccatga aaggctcctg attctgcccg cctgacaggc cccggccgcg ccccctgttg
    21841 gcattccgct tttagaactg cggggggaat ctgtgcacca gcgggccaac cggcaatccc
    21901 gggtgagggg ggatgaccgc ttccgccgtc gaaggccgga accgcgaggg aatgtcttgc
    21961 ttattcccaa gtcgctcaaa gctgagccgc gcttgccggg cagttgcatt ttgggagcag
    22021 aggacgtaat atggaggtgc cggcaatgtt gcgggtctag agtgggcgca agcccacttt
    22081 tttattgcag taaatgtcta ccatcgctga acgcgttgcg gaaatcgcac aacgggtcgc
    22141 ctcgtcggaa ggcatggaag ttgttgaagt agaatggaaa ggcggcggaa acaaccgcat
    22201 cctgcgcatc ttcatcgaca agccgagcgg cgtctcgcat gcggattgcg agatcatttc
    22261 gcagcaagtc gggacgatac tggacgtcga agacgtgatc ccgtcccggt acacattgga
    22321 ggtttcctct ccggggctcg accgcaagct attgaaagcg gctgactacc agcgctttgt
    22381 ggggaaaaag gccaaggtca agttgcgcca cccgatcggc ggccgcactc atttcttcgg
    22441 caggctcgcg ggcttcgaag ccggtgaggt agaaatggaa gcggagggcg caggccgcat
    22501 ccggtttccg ctggagaacg tactgaccgc ccggctggtg gcggagtttt gaaagcgcca
    22561 tttaccatgc caggcgagcg cttttccaga ccgtaaaatt ccagcagtca tttgagcttg
    22621 tgtttttagc gaggattcat ggcaacgcta tacgattcga tcgaaacgct aagccgggag
    22681 aaaggcatcg atgcgcaaat cgtgctggac gcggtgaaag atgccattct tgtggccgcg
    22741 cgaaagtatt acaaaacggc tgaagatctg atcgccgagc tcaatccgaa aaccggccaa
    22801 atcgatgttt ttgcggtaaa aaaagtcaca gaaccggtga cgaatccctt gaaggagatt
    22861 tctctcgaag acgctcgccg ttacgacccc aacgcgcaac tcgactcgga gatccggatc
    22921 gccaaaccaa ccgaggtgct gggccgaatt tccgcgcaga ccgcgaagca ggtaatcttt
    22981 cagaaaatcc gcgaggcgga gcgggaaacc attttcgggg agttttcggg acgtgtgggc
    23041 gagttggtga cctgtgtcgt caagcgggct gaagggcccg atctggtcgt cgaccttggc
    23101 aaaaccgagg cccgcctgcc ccggcgcgag cagtcaaaag ctgaaagttt tgctcccggc
    23161 gaccgcattc gagcggtgat caagagcgtg gataaattta gccggggccc gggcgttgtg
    23221 gtttcccgcg ccgcgccgga gctggtaatg cggttattcg agcaggaagt gccggaaatc
    23281 tatgacggca cggtcgtaat caaagcctgc gcccgcgaag ccggcgagcg cacaaagatc
    23341 gccgtgcaat cgcgcgatcg agatgtggac agcgtgggcg cctgtgtggg aatgaagggc
    23401 atgcgcgtac agtccatcat ccgcgaactg cggggcgaga aaatcgacat cattgagtac
    23461 tcggaagacc cggtggagct cgcaaccaac gcgctcagcc ccgcgaaaat cagccgcgtg
    23521 gcgatcatcg acccgatgga aaagcatctg gaggtggtcg tggacgacag ccagctttct
    23581 ctggcgatcg gcaaaaaggg gcagaatgta cggctggccg ccaagctgat gggatggcgc
    23641 attgacatca agagcgaaga ggagaaacgg caggaagtgg agtcggcgat ggcggcgctg
    23701 gtgtctgcgg gcactcccat ttccgttctg ctggaccatg gattggggga aaagctggtg
    23761 gaaaagctca gcgaagcggg cgccaccaca gtcgagcggc tgagcgacat gacgcccgaa
    23821 cagttggaag aaattccagg cattggcccg aagatggtgg aaaaaatcca gctcgcggtg
    23881 cagagctact acgggcagtt tgaggaagag cagggcggag gcgaggcgcc ggctcccgaa
    23941 tccgggaccg aagagggcca ggcagagaat caggctgccg ccggagccga agctggtcag
    24001 gaagcggcga gctctgtagg ggaggcctcc ggcgtggagt tgtctgcatc gaacgcggcg
    24061 cccaacgaat ctgataagat agcgcttccg ggccaatcgc aagccgagag tgtcacagcc
    24121 gaagcccccg gcgaggctgg cggggccgag cgggagtaat ttttgtttat atgtttgatc
    24181 cgaggtttag aaaatgatta cataaccgcg gtgaaggggt ctttgtattc ttcttatgca
    24241 aaaaattcga atcaatgaac tggccagaga actggaaatc aaagccaatc tgatactgga
    24301 gctattacca tctttaggtg tcaccgacaa gaagacgcat tccagctctc tcgatgacga
    24361 agtcgcagag aaggtacgaa gacactttca gaatggcgga cgcgggactg tcggtggagc
    24421 ggcgcaggcc gaaccggccg ctccgcccaa gcagccgccg gtagaagcgg cagcgcccgc
    24481 cgcagtcgaa gaacctccaa aacctcatac gccgatctca gtttctcatg gcgcccatcc
    24541 gttgaagccg ccgttgcggg cgggcggatc cgccctgaca ccaccgctag cgcggccggc
    24601 gccgcacttt gcgccgcctg aatctgctcc agcgccgccg gcgccaactg ccccgccccc
    24661 tgcgccagtc caggctgctc cccctgcaac tggagagcct gggcctgctg cggtaacgca
    24721 cgtgatccca cctccacccg cggtaaagcc cattcccact gcgccgaccc ctggacaagc
    24781 ccccacaggc cctcgcgctc cgctgccgcc tgccgcggcc cagccgcctc taccgcagcg
    24841 tcccggcatc gcccggcccg gcgtgccgcc ggccgcggca gcgggacctg gcatgcggcc
    24901 gccgctagcc cccgggcagc atccagccgt cagccggccg ctggcaggcc agccacctgt
    24961 gcggccggta gttccaccac gacccgatct tgctccaaag ctgcggcaag ccgtccagcc
    25021 ggggaaaccc gccgcacgaa agccgctgcc gcctccgacg ccgggtcagc ccatttttca
    25081 gcggcccgac cgcgaccgcc ccgggatgcc ggtgcggccg gggatgccgg cgggaatccg
    25141 cccgggcatg ccatcggcgc ggggagccgc gcctgccgga tcccggccca gcccccgccc
    25201 catgcacccg acctccagga cgcctcgcgt ggggccgggc gttgcgcctc ctccaccggg
    25261 cggcgctcct gcacccgatc aaaggcggcc acagcaccgg acagcttatc agcgcgcccg
    25321 ggaccgcgag caggaacaag aggcgaagat tctccaccgc cacacccagc agcggaccca
    25381 ggtctccgaa gagccgccgc ccatcacccg cgaaatcacg atcagtgagg ggatgacggt
    25441 caaggagctc tcggaaaaac ttggcgtcaa ggcgggagcg gtaatcaaga agctggtgga
    25501 ccggggtatt ttcgcgacga tcaaccagac gctcgactcc aagaccagca cggaacttgc
    25561 cgcccagttt ggcgcctcca ccgctaccac gacgtttgaa gaagaggtta tgcagaacgt
    25621 cgagctggcg gaagagccga aagacctcca gccgcgcgcg ccggtagtga ccatcatggg
    25681 ccacgtcgat catggcaaga catcgctgct cgacgccatc cggcaaaccg acgtggccgc
    25741 tcacgaggcc ggcggcatca cccaggccat cggcgcatac caagtggagc atagcgggcg
    25801 caaaatcgtc tttatcgata cgccgggaca cgaagcgttc acgcggatgc gcgcgcgggg
    25861 cgcgaaggtc accgatctcg tcgtcctggt cgtcgccgcc gatgacggcg tcatgccgca
    25921 aactttggag gccatcgatc acgccaaggc ggccaaggtt ccgatcctgg tggccatcaa
    25981 caagatcgac aagccggacg cccagcccga acgcgtgaaa cagcagctcg ccgacagggg
    26041 tttgctttcc gaggactggg gcggcgacac ggttatggtg cccgtctccg ctaaggcgaa
    26101 aacgaatctg gatctccttc tcgaaatgat cctgctgatg gccgacatgc agaacctgaa
    26161 ggcgaacccc aatcggccgg ccatgggtac cgttctcgag gcaaagctcg accgcggcca
    26221 gggacctgtc gccaccgtcc tggtacagaa cgggacattg cacgtcggcg agtacttcat
    26281 cgtgggcgcc gtttttggca aggtgcgggc cttgctgaac gacaggggcg agcagatccg
    26341 cgaagcaccg ccctctacgc ctgtggtcgt gctcgggctc gaagatttgc cgtcgccggg
    26401 cgatgccttc caggtggtga ccgatacggc caaggccaag cagattgtca cctaccgcga
    26461 gtcgaaggcc cgcgaacagg ctatggccaa gagctcgcgg atgacgctcg agagcctgca
    26521 tgaccagttg cgcaccggcg aggtcaagga actggcgatc gttttaaaag ccgatgtcac
    26581 cggctccatc gaagtgctta ccgatacgct ccagaagctt tccactgaca aggtgaaaat
    26641 caatctgatc cactcgggag taggagcgat ctcggaatcg gatgtgctgc tggcagcggc
    26701 ctccaacgcc atcatcgtgg gtttcaatgt gcggccggag cgcaccgccg ccgtgcttgc
    26761 cgaaaaggaa aaggtagata tccggctcca cacgatcatt tacgagctca ccgaagagat
    26821 ccagcgggcc atggccggac tgctcgaacc ggtcatcaag gaagttaccc tgggcaaggc
    26881 cgaggtgcgc gagacgttcc gcatccccaa agtcggcgtc gtcgccggct gttttgtaca
    26941 ggacggccgc ctgacgcggg acagcgaagt gcgcctgctg cgcgacaatg tagttgttta
    27001 cacgggcaag gtcgcttcgt tgcggcgttt caaggaagac gtcagcgagg tcaagagcgg
    27061 cttcgagtgc ggcgtcagcc tctctaattt ctccgacgtg aaacaagggg acgtcatcga
    27121 agcctttatg acccaaaaga tctccggcgg tgatctgagg gcgagctcga ggtgaaatgc
    27181 ccggtcagcc gacgattggt gtgctgacgc tggatctgca tatcgagcac tcgcattccc
    27241 tgaaagacaa acgccaggtt ctgcgcagtc tgcgcgaccg cttgcggggc cggttcaacg
    27301 tcgctaccgc ggaaatcgat caccaggata gctggcagcg cgccgtcatc gccgtggtca
    27361 cactctccgg cgaccggctg tacgtcgagc ataccctcca actggccgaa aaagaggccg
    27421 caaacctgtt aggcgcgagc ttggtagcgg ccgagattga atatctctga agcgccgccg
    27481 ggcaaagagg acctgcctca ggctccgctg ggtagcgggc ccgcattcga agcaaatttg
    27541 caccctccct atgtcatccc gccgagggag gaattcgcta ttatcctgct ccacggccgc
    27601 gcgctctgcg caaatagaaa ctgagagaac agatccttgg ctggtctcag aactggaagc
    27661 ggagcgagag ctgcaaattc cgcgccagcg aggtcttgcc ggtaatcatg ccgaactggc
    27721 cggaagttgg aacccaattg ggcgccgaca ggttcggatg attgatgaag tcataagcct
    27781 cggcccgaaa ctgaaagaaa gtcttctccg taaccggaaa acttttgagc agtccgatgt
    27841 tccaatcctg caagccaggg gcgtagattg aatcccggac gccgtgctgc aaattgaagc
    27901 ttcctgctgg cggcggtgac acgttcacgg tgaagtatcg gggcgaggcg gaggttgtaa
    27961 ccggtccagc aaaggcgccc ttgttgattg tcaccggtcc gttcaaaacc caaaactggc
    28021 cctccgggca gttgctcacg ccgaagctgc caaattcgcc cacgccggca aaatcattgt
    28081 tagtgcctat accgcaaggc gtacccgtct ggaattgcat tgcaccagct atttcccaac
    28141 ctccgagcaa cttcccggaa agccgcgcgc tggtttcaag aagggtaact ggtaaatgta
    28201 gttgatcaat agggcgtgcc gggtgtcata ttccgagggc ccccaaagat tacttgtgtt
    28261 atacgtatcc ggcacgatgt cacggtagtt cgagcttccg tccatacttt tggaaagcgt
    28321 gtagctaaca ccgagcatca gcccgttttt gaaggggcgg ctgaaagaga tctggaggga
    28381 gttgtacatg gaattgacga cgctctcctc ttcctggatg gcggcgaatc ccttataggg
    28441 gcgcagaaag ttcacattga tgccaggatt ggcctgcaag gcgccagcca gaggctgatt
    28501 aatatcgtaa acatcccagg cgtgtaatcc gcggtgcgca acgtagccca ccgacaccag
    28561 cgaatctccg gggagttgcc gctctatagt aatattccag ttccaggcct ccggcggctt
    28621 cagctggggg ttgagggtcg tcatagtcag aggggctgcc gtcccggttg tgagcgcggc
    28681 gccggggttg tcgaccgata cattggtcac cgttacgaac ggctggaatg gggaatttcc
    28741 acccgggaag atattgtcca gcagacccat acgggtcaca aaacgtccga tgccagaccg
    28801 cacgacagtc ttagcagtga gctggtaggc gattcccaat cttggctgca gtgcggtttg
    28861 acgggcgata tagcttttgg acaggtttgg cgcaaaaagg cccgaacatg gcgcgccgtc
    28921 gcaaatgttc gcactggcgg caagaacgcg cccctgggca gaactgggga acgaactaaa
    28981 gccaggaatc accaccccgt tatagggatt gcccgtgcct aggatcacgt ttccggtctt
    29041 ggggtcgacc tgcacggctt gcgcggggtt gtacagtgcg gcatcgaagt aatcggcatt
    29101 gccccagagc ggatggaaac cctgaattgt ggtaatccgc aaaccgtagt cgatgtgcag
    29161 cttctgggta gctttccagg agtcctgggc aaactcctcc cacatcccgc cgcgccagat
    29221 tgtataggct cgggggccga tctcagtgta gctgtccgcc agcccgagcg ctaagttcgc
    29281 caggcctatt ccggaagtcg cgcctaagcc ggtgcgggcg tcggtgaacg taaaggttcc
    29341 attctggttg ttactgcctc cgggcacggt gttcacattg atctgatcgc cgtcgttttc
    29401 gccggagtat tcgaaagaaa agccgaattt gaaggtatgg tttttccaaa tcttggtaat
    29461 gctgtcgccc cccgtccaaa ttggtcctga agaatgggag ggatacgggc cgcctgccag
    29521 gctgtaaaac ggcgcgggaa cgttgactgt tgggatcttg ccaggaatgt cctttcctcc
    29581 gaagaggtag ggataatcga tgccgaacac cgagcggttg aacccgggca agctggcgtt
    29641 caccggaatg tagacatcat ctaggctgaa ggaaatacgc gcttcgttga ccagcgtcgg
    29701 attgaccgtg taagtccaag agatcgtgtt ggtctggttc ggccggttga agtacttgcc
    29761 ggtctcgccc gagccttggt cgaagggctg atattcaaaa tattgagcgc ctgatttccg
    29821 cccggaaatg tgttgtctgt ccgtggcctg aatatcaata ttcagagtgt ccttccgctg
    29881 gttgtatggg tgcgcggctt gcgcgatcca attctgattc ccctgaagaa aaccgggcgt
    29941 aggagccggg tatgcgctca ggatcgcgat gccgttgtga ctgagccggc tctggggaat
    30001 gatgttgttt gggaagggct ggcaagaaac cgcgcctagg ctgggacagg tcgtcggatc
    30061 gtaaattaga tgcgtgccgc tgtaaaaggg gttaggtccc agcaactcgc tgaaatttcc
    30121 ctgacgcatc agaagcgtgg gaaccgcctg ggtttgcgta tccgtattgc gattgcggat
    30181 ccagtcttca gcgatgaaga agaagagctt ttcgcgccat ttccggttga gccagatggg
    30241 gccgccgacg gcgaatccaa agttgttgta ccggaacggc gtggtgaagt tagtcgtcgg
    30301 gctcaggttc cgttgccagg tgttggcgtt caagtcggaa ttgcgaaagt actcgtacaa
    30361 gctcccgtgg aagtccctgg tgccgctttt gctgaccacg cggatttgcc cgccagcggc
    30421 ccggccgtat tcggccgcat aatcggccgt cagcacttgg atttccgccg tcgagtccac
    30481 gtcagcactg ccaatgattg cgccgttaga gcgggtgcgc acggccggtg cgccgtcgaa
    30541 cgtaacaagg gtgtcccaaa cccgcgcgcc attgatctgc cacgactggc cgcctgtggc
    30601 ggcgaagttt aagtcgccga gcgtaatccc gttgttgcca tgcacgccgg gtaagagctg
    30661 gaccatgaaa atgggattgc ggccgttcaa ttcctggtcg agcacctgcg tgcctgtcac
    30721 ttccttctgc acggtagctg actcggtttg cagcgtggcg gcggccgcgg tgacttcgac
    30781 tgtctcagtg gtcgcgccca gcgtcagggt gatatcggtt gacagcgtcg agttcggctg
    30841 taaccggttt tgcccggact ggaacttttt gaaaccctct ttctccgcca tgatcgtata
    30901 cactccgggc ggaagattgg tgatcacgta gtagcccgat tcattggtct cggtcgatcg
    30961 ctcttggccg gtggcttcgt ttcgtatggt gaccttcgcc cctggaacta ccgcggagga
    31021 agggtcttta acgaaaccag tgatggaccc ataatccaac tgcgcgaaaa cgattccggc
    31081 cgtcagcagc ataaggatgg cggccgtaaa taacctggcg gttacgcggc gcaaactgca
    31141 catatacaac cccaagcaat taattgacat caatttaatt cctacgcgtc gtgttgtcaa
    31201 gctcgttgtc tcagaccttt cgtcaaatac aatccaataa ccacgcaccc gagcactaga
    31261 ctggcgcaaa ttctgtttcg tcttttctgt gtgatatcgt ggttttgtgc aggaaaaacg
    31321 catcgagcgg gttgccgagc agctccgctc ggaactggcg gaactgatcg agaacgagct
    31381 ggctgacccc cgcctggggg cggtcaccgt caccagcgtg cggctctccc cggacggcaa
    31441 acaggcgcgg gtcaatgtga gcccgtccgt gcagaccggc ggcaaatcgg aagagcaatc
    31501 ccaccgccgg gacgaagaaa tcctgcgcac cctcgatcac gcccgaggct ttttgcggca
    31561 cgagctggcc tcgcggctcc ccctgcgcca catccccgac ctgaaattct ccatcgacca
    31621 cggcccccag cacgcccagc gcatccagac gttgctcgaa cgcatcaaaa accgcgcccc
    31681 ggcagccgcg cttctgctcg caagcgcgat cttgggccgg caggcattgc tgggcgccga
    31741 gatgctgcgc tacgaaaact ccacgtcggc catgggcagc gtgtacacaa tcgccgctta
    31801 cggggaagac cgggcgcagc tttcagcggg agtgggcgcc gccttgcagg aagcccgccg
    31861 cctggatgac cttttgagca actacaaacc ggaaagcgag ctgagcgaaa tcaaccgcga
    31921 ggcttcccgc cggccggtta aagtgtccgt ggagctgttt gaactgctcg aaaaatgcca
    31981 gcagtacagc aggcttagcg agggcgcgtt tgattggact gtcggaccgc tcatgcgtat
    32041 ttggggcttc tatcgcggga cgggccatgt gcccgagccg gcaaagatcc aggaagcgct
    32101 cgagaacgtg ggctatcggc acattcatct ggaccctggg tcccgcacgg tgagttttga
    32161 caagccgggc gtcgaactgg accctggcgg catcggcaaa ggatatgcgg tggacaagat
    32221 ggccgatgtg ttgcgcgacg ccggcatcca ttcggctctc atcagcgccg ccgggagcag
    32281 catttatgct ataggcgcgc cgcccggaga gaagggatgg cacatccgca ttcgcgatcc
    32341 gaagtccgat gagataacgg ccgccgaggt cgacctgaag gaccagtcgc tttccacttc
    32401 cggaacctac gaaaaattct tccaggcgga cgggaagatt tacagccaca tcatggatcc
    32461 gcgcacgggc tatcccgccc aaggcatgtt gtcggtttcg gtcatcactc cgaaaaccat
    32521 cgacagcgaa gcctggacga agcctttctt tgtgaacggc gccgcctgga ccaaagcaca
    32581 cgccccggaa ggcttccgga ttttcttgtg tgaggataac ggcaagccct gttactggat
    32641 tccctgacat cagaatgcct tgaaagcgtc tcgaccttcc tcactccttt ttcggcaatg
    32701 ctcgcgccga gagcctgcca aggccgatcg cccggcaacc ggcggtctgt aattgccgat
    32761 tcgatacaat gattttctgc gcttgagccg cctcgttcga atcctgcttg ccacgctagc
    32821 agcggccgtg ttgggctgct ccgcgatctt ctattggatc gccatccggc ccctggccgt
    32881 caccagcggt tcgatttcgg gtccccgatt tccggacgcc gtcgcgggac gcgaccgcct
    32941 ggatatcccc cacatcgaag cccattccat ttctgacgcg ctctgggtgc agggctacgt
    33001 caccgcgcag gatcgccttt ggcaaatgga catcttccgg cggctctctg cgggtgagct
    33061 ctccgaagtt ttcgggccgt ccatgctcga tcgcgaccgc gagagccgta ttttaggcat
    33121 gaagcgggcg gcgctcgccg cccgggagcg tttgcctgcg gaggatcgcg ccctgtttgc
    33181 ggcttatgcg gccggcgtga acgcgttcat cgagtctcat cgcggcaacc tgcccttgga
    33241 atttcatctc ctcaattacc agccgaagag ttgggagatc atcgattcca ttctcgttgc
    33301 catgcatatg tttcgcaccc tgaccaatag ctggcgcgac gagatcatga agcgatctct
    33361 gctcgagcgg gccacgcccg aacaggtcca ggcccttttg ccggtgcgca cgggccgtga
    33421 atttgcgccc ggctccaacg cctgggcggt ttccggagcg cacaccgcga gcggaaagcc
    33481 gctgttcgcc aacgatcctc acctcgatta ctcgatccca tgtatctggt acagcgtcca
    33541 cctgaaggcg ccgggattga acgcaatcgg ggtcacggtg cccggcgttc ccggggtgat
    33601 tctcggccac aacgagcgga tcgcctgggg cgccaccaat ctcggcttcg acgtgcagga
    33661 tctctatctt tcaccagccg ccgagcgggt tgagcaggag attatccacg tgaagggagc
    33721 ggccgacgtg gtgctgccgg tgtccttcac tgcctatggg cccattgtgg ccgaggtggg
    33781 cggccaaaaa gcggctctgc attggacagc cgccgatccg gctgtgtggc agtttccgtt
    33841 catcgagatt gaccgcgccc aggattggcc aaccttcatc cgcgccctcg agcgctacgg
    33901 cgggccggcc cagaactggg tttacgccga tgtagacggc aatatcggct atcaggcggc
    33961 cggcaagctc ccgatccgca aaggattcga cggcagcgtt cccgtccccg gcgacggccg
    34021 cttcgattgg cagggctata taccgttcga tcaactcccg cgcgcttaca atccccagtc
    34081 gggcatcatc gtgaccgcca accagaatcc gtttccgccc cgctatccct atacagtgag
    34141 cggcaatttt gcttcgcaat tccgcagcaa tcggattcgc gagcttctgc aaaacggcag
    34201 ccggttcgaa ccggccgact tcctgcgcat cgagacggat gtgtattccg cgttcgccca
    34261 cgagtttgca gaaaatctcg tacaggcgat caaccggcgg aagccttcag agccggatct
    34321 cgtctctgtg gcagcccggc tccgcgcctg ggatggccag tttcgcgcag acgaatcgat
    34381 tcccgtgctg gtggcgatca cctataaccg gttcctccgc agcctggtcg aggccgccgc
    34441 cggcgaccgc gcggcgctct atagctcgca gatgggcacg gctttcgtcg cgcacgtgct
    34501 ggcgacgcgc ccgccgggat gggcgaagga ctatgacgaa ctgctcgtgc gctgcctcga
    34561 gcgcagcatt catgatttgc gcggcatgcc gcccaggtcc actgccacct ggggacagta
    34621 tttggagacc actctcactc acccaatcgg gcgcagtatc ccggtgctcc ggcgctggtt
    34681 tgacatcggc ccgttcccgc aatcgggtac aacgactacc atcaagcaga ccaccacgcg
    34741 catggggccc tccatgcgca tgatcgtgga tctttccaat ctcgataact cgctgcacaa
    34801 catcactacc ggagagtccg ggcagccttt ctccccccat tacaaagacc aatggcgggc
    34861 ttattacgaa ggccgcagct tcccgcttca gttctcttct cccgaaatcg aggaccgcct
    34921 gctattccat ccggcgccct gaatcgagat tttggccaaa ccggccgata agaagagaga
    34981 ggcatgaacc acgagcgctg gctggtctct tatgcggact tcatcaccct gctgttcgcg
    35041 ttttttgtcg tcatgttcgc ctcatcgcag gtcgacaagc gcaagatcgc tgcaatggcg
    35101 gccactttcg attcctacgc cgccggcggc gcgcaggcgt ctcgagggaa cagccgcccc
    35161 gctccctccg cgcaagcggc cgaagccgcg gccccgtcca gccagggggc cgctcccggt
    35221 ctaacaatgg ccgagctgga accatccaga gacaggctgg cggcagtcct ccatcgggaa
    35281 atcacttccg gagaagtcga gatctcgctt gagccccggg gcctggtgtt gagcttgaaa
    35341 gattccgcgt tctttgctcc aggcgatgac gccgtctcac ctgaggcgcg cccgatttta
    35401 gccaaggtgg cccaggcgct ccggcaagtg ccgggagaaa tccgattgga aggccatacc
    35461 gacgacaggc cgattcattc gggccgattt ccctccaact ggcagctttc cactgcgcgt
    35521 tccatagccg tattgaaggt ccttactggc gagctgggat ttcccccgga acgcctcgcc
    35581 gtcgcaggct atggagaata ccagccgctc gaatccaacg agaccgagtc aggtcgccgc
    35641 aagaaccgcc gggtggattt ggtcgtactc acacaagagg cggcgtctat ggcgccgtcc
    35701 tgatccgcag gggatcttcc gcggcctcat ctcacggtca cttttgccct caagttcgcg
    35761 atccccgcgg tataacggct gtcgatcact gagaagagca gagcgtcctc cagccagtcg
    35821 tgctcggcgc cgttcgggtc gtgtgacgcc accgtcacgg tgtactcttg cggacagaga
    35881 tcgcaggcaa actgaaattc gaccgtaacc gcctcgccgg cgcggcggaa gccaattggc
    35941 gtctgttcga gttccgtgtt cgttccatag acgcagatgc cgatgcggtt tcgaatcagc
    36001 atgccggcca ccgggttttc gaccggctcc aaaaaacgca attcggctga cacggtgatt
    36061 gattcgccgc tgcgcactgt ggtcgtgggc ctgccggatt cgtctcgaat cttgatcgcg
    36121 gccacttgcg cgcggccgtc gccgcggcgg gaggacggac tcataccgtt caaagccccc
    36181 gccagccggg tgtgctccaa tatgcgccgg cgatagttcg ctaaaaccag cccgggatct
    36241 ccctgctcca cgaggcggcc attgtcgagg gcgatcacga catcgcagat ccgctcaagc
    36301 aggcaaaggt catgggaaga aatcagaatc accgcgcccc tgcgccgcaa tctgtgcaac
    36361 tgctggatgc agtggatctg cgcggcgccg tcgagcaacg ccagagcgtg atcaataagc
    36421 aagaccttcg tagtggcggc agtggcatcg gcgatttcct gccgtattcg tgcgggaccg
    36481 ccggctccca ctcggatcaa ggccctctgc gaaggcgctt ccacgcggcc ccgctcggcg
    36541 gccgctgcgc cgcccgccag ttcgaggagc aggctcttcc ccgccccgtc cggccccacc
    36601 acgccggcga tcgtggcatc gtcaaaacca agcgatatgc cggccagagg cggcagatag
    36661 acgtcatcaa attgcagcgg catcgcaaac tgccgttata acacggcccg ctgagaagcg
    36721 ccgaaagaag gccaatcaag cctgcgtctt catcgcccgc cgcgggcgtt tgctcggcgg
    36781 ggaatcggga ccattcggat tgctgcccgc aagacggcct gcttccgcag aaagagcctc
    36841 tgacaggcgt tatatcatga gggaatggcg gagccggaaa tcaccgagca gatgcgcgaa
    36901 gattgggacc gccgcgcccg cgaggatgcc gcttactatg tagccttcgg cggacgccgc
    36961 cagtccccgg aggagttttt ttccaccgct gcgccggtgc ttcagaccct tcgatccgag
    37021 ttccggcggt tcccgtcggc tcctaaaacc ctctccgcgc tcgagatcgg ctgcggtccc
    37081 ggccgcctgc tgattccatt gagccgcgat ttcggccgca tcaccggcgt cgatgtttcg
    37141 ccggaaatga tacgtagggc ccggcggaac atcgagagca tcccgaatgc cgccgcagaa
    37201 gtggtttcgg gttccgattt gtccggtatg agagatgagt cgtttgattt ctgctattcc
    37261 tatgccgtgt ttcagcacat accgagccgc agcgtagtgt ggaactacct cagcgaagcc
    37321 cagcgggtgc tcaagaccgg cggacttttg aagtgccagt tcaacggcct tcccgagacc
    37381 gaacgacggc ctgccgacac ctggagcggg gtgcgcttca gcgccgggga acttcgagcg
    37441 tattgccagg aacacgaact gcaattgctc tcgctggatg gagcaaatac ccaggatctc
    37501 tggatctgcg ccaggaaaca gccgccggga tggacccggt ccctcgcccc cgaggatgga
    37561 gtccatttgg tagaagttgg caacacctat accagcgacc ggctggttcc cgtctcggga
    37621 cgtttttcgg ccgtctcgct gtgggtgaaa aatcttagtc cgggtgcgga catcaataac
    37681 ctggaaattg agataggcgg gcgtaagact cttccctgct acgtctctga ggattccggg
    37741 cgcgcgcccg tgcagatcaa tgcttttcta ccggccggac tgccgacagg cttggtgcag
    37801 gtccggctgt ggatgctggg gcggccggtt tcgaatttcg cccggctgcg cctgattccg
    37861 ccgcctccac ggcgcccgcg gctgcttggc gtaaccgacg cgatcgaatt gctctccccc
    37921 tttcgcatcg aaaccggctc tatcaaagcc gaggtggagg aggcgggcct atccccggaa
    37981 atcgctgcgg atatcgacgg cgtgccggct caggatattg agatacgctg tgtgaatccg
    38041 ctctcggagc attacgccgt cattataaag acgccttcgc agatcggacc ggggcaacac
    38101 tcgctcaacc tccgcctcca tggccgcatt ttgcctgccg tgtccatcga agtggcccga
    38161 taatggggaa cccgttcttt cgttttcccg cagcgctggc tgttttgctt atttcgctag
    38221 tgatcatcgc cccatacacg ctgggccgat ctgctccctc tgcggacgcc gagctggcgc
    38281 gatggtatgc gcgaaaccca aaaccatggc agtggcaaac cgagtggggc ggaggttcgc
    38341 cggttctgag tttgggatcg ccgggtctgc ccctgctcac agcggcggtt gcttcgattg
    38401 ggctgccctt tgaacgtgcg aggctgatcg ccggggggct cagctacgct gtggctcctg
    38461 ttggcgtatt ttatttggcc cgcgctctgg cgctgccggc gtggtggacg tctctggctt
    38521 ttgcgctggc gccgcaccgt tggctcgatt ttgcgcggca ggacaatgcg gcgcatgcgc
    38581 tggagcttgc tctgctgccg atcattttgc ttcagttcgc ccgcagacgc tggctggccg
    38641 gagcgggcgg cgttctggca gcggccttgt gcattcctcg aagctggaat gcactgccgg
    38701 aactggagct ggccatcgct ctgttctccg gcctcctggc aaagcacgcc aagcaaatgc
    38761 ttcccttggc tgcgtgcatt gcaatcttcg cggctggcct gagctgggag gccttttttc
    38821 agcgctcggg agccgttcca ggcacctcct tcagcgtacc cgcctacatg gcttcggggg
    38881 agctgggtga gacggttctc gggcttccgt tccctgatcc aggttggaac ccggttcgcc
    38941 ggcaggcgga gcagttgatt ctgagcccta accgtcccat ggattccatt ctctggatgc
    39001 aggctctcgg catccggcgg ctcgctgggc cctggggcgc caagtatgac gcgttgctcg
    39061 cttgcgaaga ggccggaaag gccgggtgtc ttttttatgc aactccgttg cggcgcgcgg
    39121 cgcaagctct tcttgtctcc cgctaccagt ggcggcgcct gccggctttg cgaagcctct
    39181 atgatcgcgc ggcgctcaat gcctacgtga actggtcgaa tcgtcccgaa gcggccggat
    39241 ggcgttggac gagaagcggg acggccgaga ttcgcgccag cctggggccg gacgacatga
    39301 tattggttcg acaaaacgcc gacggcggtt ggacggcaag cgccgacacg ggtccggttg
    39361 ctcttttccg cgacccgatc gggtacctgg tgcttgatcc cggccgtccg ggcccgattt
    39421 tcatccagct cgcccgccgc acgcaaatcc ggagctcgtt cagccatccg cctcgccggc
    39481 ttccggcgcg tatcattccc gccatcgacc ccgatgggat tattgacggc gtcgcacata
    39541 cgccgccgcc ggtcgctccc ggctcagtaa tcagcatttt tggatcggac ctgaacggcg
    39601 ccgggagccg gctcggctcg acccgcgtgc tggtttccgg ccgtccggcg gaagtgctct
    39661 acgccggaga caaccagatt aatgcgcggc ttccggcaga cctcccctcc gggcgtgctg
    39721 agattatcgt cgaaacagcg gaaggccgca gcgatccggc tactctggag atccaaggca
    39781 gatgagcgtc cgatgccgcg ccctgctggc aagcctctgg cttcttcttg cctggccggt
    39841 gtgcggctcc ggcctcgcaa gccgtctcgg tttttaccag tgggatggcg ttggcccccg
    39901 ttcccctggc gctgatttgt tgacccaggc gcagcgctat gcggccgaaa ccggagcaag
    39961 ggtcttccgg ctttatctgg gcgcccgctt cgactactgg catccgcccg ggtcgccgct
    40021 cacattcgcg gacctgcccg aacacagggg acgctcccga gaggagctgc tcagcccggc
    40081 gcgaattctg gctttgccgc gttatcgggc tgcgcttcac aacgcggctt tcgaaaccat
    40141 cattctgacc acttattccg tcgcagatta cggcggcggg cctgatgatc tgaacctgtt
    40201 ccgcccctgg accgctctgg aagacggcat cgagcgccgg cagatccggg aactttgcga
    40261 cttgctctac gacgaaatcg gacaatcggc gaagacggtc gttgttgcca attccgaagc
    40321 agatgaaaag atgctggaaa tcatgaatta cacagggtcg ccggagcgcg ccattcagaa
    40381 tatcgccgcc tggacccgcg cccgccacga agcgatcgag caagcgcgcg cagcccaccc
    40441 ccaggcgagt ttgcgcctcg tccacggttt cgagatctcg ctggtgaatc tgaaaattgc
    40501 agccgaggca ggcttgttcc gcaaaagccc ccgcggcgct tggagcgccc tgtacaacgt
    40561 cgtgccgcag gttccctttg acctattgct ctattcgagc tatgaatcgg taaatgcgcc
    40621 cttcgaaacc cataacgccg acgtcaatcc catgcagatc gctgtgcggc tccggcgcga
    40681 tctggacgcg attcgcgatc actcccgcgc ttctctttca gtgacgggaa aacgtttctt
    40741 tggagagcgc ttcgtggcgg tgggcgagct cggtttcgcc cgcgaacgct ttgagcagct
    40801 cccaaccggc ggtgtgctgc cgaggctcat gacggccatc gagaccgcgg cccggtgggg
    40861 ttgtccctac atcgtcatct ggcaggtttt cgacgcgcgc cgcagcggcg gccagccgtt
    40921 cggattcggg atgatcgacg ctggcgggaa tgcgccgccc cttcgcccgg ccgccgatag
    40981 atgcaactcc gtgcggactt gcgtgcagcg cttgctcgag cccaaaagag ccggtccgct
    41041 tcgccggaat tttccggccg ggcagtagcg tctgccaatg ttccaggccg gcgtccgaag
    41101 cccgcggtct cggcacatgg ccgcggccgg gccggacttc ccgcccggag cactccgatt
    41161 tccgaatctg ctagactccg aaaaggcgtg ctgacatgag aatgctggtg gcaggtgaat
    41221 ggatcgaggg aagccagaaa atccctgtcc agaatccgtt tgataactcg gtggtagaca
    41281 ccgtgccgcg cgcctcccag gagcacatcc agcgagctct ggagttcgcc gcacgcggcg
    41341 cgaaaaccat gcgcgaactg cccggctacg agcgctacct gcgccttcgc cgcgccgccg
    41401 atttgctgga aagccgctct gaagaatttg ctcgtaccat cacgctcgag gaaggcaaga
    41461 tcctggcgga agcccgctac gaggtctccc gcgcagtcgg cacgctcgaa gaatcggcgg
    41521 aagaagcgaa gcgcctttca agcgaggtcg tgccgctcga tggcgcgccc ggggcggcga
    41581 atcgttttgg cttcacgatc cggattccgt gcggagtggt cgtcgccatc agccccttca
    41641 attttcccct gaacctggtg gcgcacaagg tcgggccggc cctcgccggc ggcaacactg
    41701 ttatcataaa gcccgccacg gatacgccgc tctcggctct gaagttgacc gaggtgctcc
    41761 tggaagcggg catcccaccg gaaggcattc agtgcctgac cggttcgggc ggcgaaatcg
    41821 gcgatgcgtt gtgctccgat catcgggtga ggaagatcag cttcaccggc agccgcgatg
    41881 tcggcgagca tatctgcaag atcgcgggct tgaaaaaagt cacgatggag ctcggcagca
    41941 attcgcccct gatcatcctg cccgacgcca atctggaaaa agccgcgcag gcgatcgccg
    42001 ccaccgggtt cgccaacgcc gggcaggtct gcatcagcac gcagcgggtg attccgctgc
    42061 aaccgattta cggcgagctg ctggaggcgg tgaagccccg cgttgaggcg atcaccactg
    42121 gcaatcccct cgacgccaag acccgcatgg gccctatgat ccgggagcat gacgccaggc
    42181 gggtcgaaga atggattcag gaagccgtag cggccgggtc gcgcgtcgtc gccggcggag
    42241 gcaggcaagg cgctttgcac taccctacta tcgtggccga tgtccatccg cgcctgcgta
    42301 tatcctgcga tgagttattc ggcccggcgg tcgccttcac gcctgcgtcc agcatcggcg
    42361 aagcgatcgc gctggccaat gacacgcggt acggcctctc ggccggcatt ttcaccgaaa
    42421 gtctggagtc cgccatgcgc ttcgcgcgcg aggtcgatag cggcaacttg cacatcaact
    42481 ggggaccgca gtggcgcgcg gatttgatgc cttatggcgg gctcaaggag agcggtttcg
    42541 gaaaagaggg gccccattac gcgattcagg aaatgacgga gctgaagatg gtcgtgatgc
    42601 accttggatg atgcttccag gcgcatccgg ctccccggcg ccggggtgag tttgtgcgtc
    42661 tccctggctc aacaatcttc tttatgagac gaattctcgt ggctgggctc ttgctggtga
    42721 ccggcttgtg ggctcgctcg gcccccaagc gggatgtcct ggacccgctg tccgttttct
    42781 tccgccatcg cttgcacgaa gcgcggcggc aggcgcagcg cgctctctcg cccgagcagc
    42841 cggtcgaagc ggtgctgccc gacgccggcc aaatcgcgat gatcgacgat tcggacaacg
    42901 tcgtgctcgc cggcttcgat ctggatcaga aaaccgtgga gtttaaaccg gccgccgcca
    42961 acgcggcgca gtataccttc gacgtggctt cctcgcgatt cgacgccagc gtgcttgcct
    43021 cctctaccca gctctcgttg ggcgacgacg attcgaagga ggttgctctt cccttcattt
    43081 ttcctttctt cggatttcgt tatgccagcc tctttatagg atccgacggc cacatttcgt
    43141 tcgggagagc ggacgaccgc tcgtggcaga gcggcgaaga ccccggcgtg cgtctcgtcg
    43201 cagggccgcc gcgcctggcg ccgctttaca ctgatctcga cccgtcccag gccggcgccg
    43261 ccattagcta tctgtcttcc gcctccagat tcgtaatcag ttggcagaac gtgccggttt
    43321 acagccccaa ccccgggccc tcggtgggcc gccagacttt tcaagccgct ctgtatccgg
    43381 acggacgcgt cgaattctcc tacaacggaa tccatgtggc cggtgcgtcc gccgtcgccg
    43441 tcggcattgc ccagggaggc gatataaacc tcgctctcta cactcttgtg gatttcgcgg
    43501 ccggctccgc ggccactttc aaaagcacaa tcgtggagga attcattaat accccgcagt
    43561 tcgacatagg cggagtggcg gccaagttct tccgcaatca cgaagatgct tacgattatc
    43621 tgatcgtgct cgacaccgtc aaccggggtg accagatcgg gggcgcctgc gctttcagca
    43681 ttccggtcca caattggatc cacggcatcg gcctcactgt cagcgccacg ctgccgatcg
    43741 aggagttcga tctcggcggg acagtcggcg catccgggcg ctttcaggac ctcgtgtatc
    43801 tcggacctct gagccaatat ccggccaatc ccagccaaat gctgccgagc ggcgggattt
    43861 gcgggcccaa cagcacgctg agcgttctag ggcaggaatc cggacaccgg tttcttgcct
    43921 atttgcaatt catagatccg gccacggggc agccgtccgc cgatctgctg gggcgcgact
    43981 tccagcactg gagtttctac ttcaacagcg acgcctcggt gatggagggc aaccagatca
    44041 tcgaccacgg cgcaggcgct tccccgcgct tcgagacgca aaaaatcaat cagcactaca
    44101 gcgctctcga cgagtacgca atgggcttgc gggccgcctc cgacgtgccg ccgtcctttc
    44161 tcgtgcgcca gccctccata gactttccgc cggaccatcc gccccagccc ggaatactgt
    44221 tcaatggcag caggcaggat atcaccctcg acatgatcgt cgcggccatg ggccggcgcg
    44281 ccccggatgt gaccctttca caaaaacggt tccgattcgc ctttgccctg ctggcgccgg
    44341 cgggcaccac gccgtcgcag gccgacatcg caaaggccga cgcgattcgg acagcgtggg
    44401 aaagttattt cagcagtatc acggataacc gcgcgcaagc agacaccagc ctggtcaaac
    44461 aagtgcggct ctcggtttgg ccggtctcgg gagtgattca gggccataca gtgagcgctt
    44521 ccgttgcgct gggcgccccg gccggcgcgc cggtgaccat cagcctcaac gcttcgggac
    44581 cggcgatctc gattcctgct tcggtcacca ttcccgccgg cgcgcagagc gcctcgttca
    44641 cgatcgcggg gaactcgccg ggcgccgtcg agatcacagc gcagggcccc gacgcaagtt
    44701 acgaaaccag ccgggctttt ctgcttgtgc ggccgaatga agccggcctc actctggaaa
    44761 gggtcagcgc ggcgctgctg gcgagcggcc tgatcgctcc ggtgacaagc cagacagcga
    44821 gcggcgggat gggcccgaag ctggacgagg acctcgcttt tcgcgtccgc gacgacaact
    44881 acgttcctta tcccgggttg cagttggcga gccaagccag cggcacgggc gttgtagtga
    44941 attccagcct ggtaacggat gacctcggcc gcgtgcgcct tacctggaaa ctcggctcca
    45001 cgccgggaat gaatacgtta accgtcatgc tcgccgacca gccggacgtg tccactcagg
    45061 tgcaggcgat cggcgtccct gaaccggcgc acctgcgcga tctgcgctag cccaagctgc
    45121 cgaaaaacgc ttccgtccgg ctgcgggcgc ttcgcttggc ttgcaggccg cggaagtctc
    45181 cgccacagcc gtcaaaacat atagcgcgcg cccagttgga tcacccgggc gccgcctaac
    45241 acggaagtga cacggccgaa ctggaccgag ttgatggatt ggtcgcccga ataaaagatc
    45301 gggtgattga gaagattcgt gaacgtcgcc tcgagccgca cgcgctggcg ctcggtgagg
    45361 gcgatttgct tgtcgagctt caaatcgaag gcgaacgccg acgggccgtc aaacatgcgc
    45421 cgttgcagcg tgcccaactg gcccggctgc gggttgaaaa agacctgtcc tgcaaagggc
    45481 ggcgcaccgt cgctgttgac gccggtgccg tcggccggat tcaacgccgc aggattgaca
    45541 atgtacggac cgttgccggt catctgaaag tgcaccacct ggctcaactg cgatccgttc
    45601 agaagggtca cggcagtatt ttcacccgac cgggacgtac gattcagcgt gccgcgctgc
    45661 gagagaatcg agaagggcgc tcccgattgc caagtcgtaa tcactccgag cgtccagtcg
    45721 ctgagcagcc ggtccaccgg gccatagtgc agccggtgat tcgggcccgc cggcagcggc
    45781 accacaaagt tggcattgaa gacgtgagta acgtcgaaag ctgcgcgcgc ccgctcgatc
    45841 tggccgttgt tcaagtcgag aaacgggtca aaccgcactt ggctgccgcc gatcgagtcc
    45901 gtcaagactt tactgaagct gtaattggcc tgaaactcga cgccgttggc cgcccgccgc
    45961 cgcacttcca cttgcagcgc atggtagctg ctgttggaaa aattcgtgat cagatcggaa
    46021 acgaacgtat tgggattggg tacaaactga atcgggccgg tcaggccgtt gacataataa
    46081 atcgccgcga gctgccccac ttcgcccgaa cggatgagcg attgcacgat gccgttgtcc
    46141 agcaagcctc cgaacggaag ctgcggaaag atcgtgagcg gctggctgcc tgaaatgcgg
    46201 gaattgtagc tgggatcgaa aacgccggtg cttgcttggg aaaggaaggc gttgttgcgg
    46261 gcgcggatga agtcgtcaag aaatccgttc tgcttgacga tgacctggtt gtaatcgaag
    46321 cctcgcagca gtttcgtgcc gtgattgccg acatagcgcg cttcgacgac cgtgtttttc
    46381 gccacttccc gctggatgcc gaaattccat tgctgcacat agggcgtgcg cagttgcgga
    46441 ttcaccccga aaatcgctga ggtcgggttt aacgcgaaat tgtcgctggc tagaagcggc
    46501 atctgatagg cgggaaccgg aaacgccggc aaattcccgc tcatggcggc taccagatcg
    46561 gtattggtga tagttgactg caagccctgg ttttggaagc tggcgttttc cgaagcgctg
    46621 atgatctcgt cgttgacgta attgatgctg taaccggccc ggatcgccgt cttgccgtcg
    46681 ccgaaggggt cgaaggcgat cccgacgaca ggggcgaagt tattcaggtc tttcgcgtaa
    46741 agcggacgcc ccaccgcgct gcccgccagg tccagggtgg catcgcttag cagcgtgccg
    46801 cgtactccgg cgctggtgaa gatgggaccc atcatcaggc cgtcccgctc gtcaaaacgc
    46861 cccatgtatt cccagcgcac tcccaggttc agggtcaggc gccgcgtgag tttccaatta
    46921 tcctgagcgt aaaaagagta gttgttcagg ctgtaatgcc gccgatactc ctggccgggc
    46981 acaaagcccg acgtgcggct ggcgacattg aacgtttgcg aagccgaccc tatgatcccg
    47041 cccaggctgg tgagcaagcc tgtcgcctcg tcaaggctgg tcgtcgaaat gcccccggga
    47101 aactggctgg ggtcgagcgc aattggattt tccgggctca gtccgatgtt ataagcaggc
    47161 agcaaacctg ccttatcgta cggggacgcg gaaatgtgct ggatctgagc gccgaaccgg
    47221 acgttgtgcc tcccgcgctg ccagttggcg ttgtcctggt agttgtatgt gttcgtggcg
    47281 cgtccctgcg gctgaaaacc gacaattgga ttggtgaaaa tgaaaccgct gaagagttgg
    47341 cttccggttt gctccgtgct ggtgaaggtt cccggcgcaa tgttcatgcc gccgcggacc
    47401 tcattggtca aagaaggcag cggcgtccat cgccaggcca gcgaaagaaa gtgcacatga
    47461 tcgtgattct gtatgacggg cacagcgtga aagccgttct cgacgtcggg ccgatcgacg
    47521 acatctgtgt tgtagtgata ggttccggag aaaatgtgct tgctggaaag cagataatcc
    47581 aaccgggttg ttacgttgtc gcgatcggtg ttatcccgga cgttaaactg atagccggcc
    47641 gtattcagcc cgtcgcccac gttgaagtta ttgatattgg cgggaccggg gacgcgctgc
    47701 aaaagcgccg ccgccttcgg atcgggcgaa acgcccgcca ggctcagaag attgacctgc
    47761 tccaggcggt tgcgcgagtc gcggtatgtg aaaatgccgt tgcgcgcgct ggcggtcaga
    47821 atcgtcgtat tgtcaagcaa ctcatgccgc tggcgatagc cttcgtaatt ggcgtaaaac
    47881 agcagcttgt ccttgcgaat cgggccgccc agcgttccgc cgaattggtt caggttcagc
    47941 agcggcttgg gagtgcctgc ctggttgctg aaccactggt tggcggccag cgcattgttt
    48001 cgattgaacc agtaggcgct tccgtgatac tggttcgtcc ccgagggcgt aacaaaattc
    48061 acttgggagg ccccctggcc gagcgccgca ttgccgttct gcgtggtgat ggtgaattcg
    48121 ctgacctcat ccattaaaag gttgttcggc aaaaaatcca gcgcattatc gcggatatag
    48181 ttgtcctgga tgttgatgcc gtccagggtg acattggaaa aagacgttcg ctggccgttg
    48241 atgaccgtgg gcgtattgcc gttgtaggcc acaccaggct gaattgcgat cagcgtcagc
    48301 gggctgcgat cgctgagagg caggtgctgg atctgctggg tggtgatggt agtggccagt
    48361 tcggcattcg ttgtctgcac ctggccggcg ccgcccggga cttcgacgac ctcggtaacc
    48421 gaaccgatct ccagccgcac cggaggcagg acgttgtcct tcgccacatc cactttgaaa
    48481 cgccggatca cctggcggcg gaatcccgtt gcgttcacct cgaccgagta aaacccgggc
    48541 ggaacctcta cgaatgtaaa ggcgccctga gcgttggtcg ggctggaaaa gctggcgccg
    48601 gtggcctcgt ttctcgcgcc aaccgcggcg ttggcgaccg gcagccccgc gggatcaatc
    48661 acggttccgg ccaggcgccc ggtggcggtt tgcgccggga gcatcgcggc gaagccgagc
    48721 agccaacaca aaatatagct gaattgtttc atctttcccc caagcttttt aagcatagtc
    48781 gagaaggggc gcgcaggcaa tccggcttct tgcctatgct acaatggcgc gctttggagg
    48841 ccccatgcgg gtggcggtcg gttttgatca cggcggattt ccgatcaaac aggatattgt
    48901 gaatctgttg aagggcgccg gccacgaagt gaacgacaag ggcgcatatt cttttgacgc
    48961 cgccgacgac tatcccgatt tcgccatcgc cgtggcccgc gccgtggctg cgggagaggc
    49021 ggaccgcggc attgtggtct gcggaagcgg cgtcggcgcc agcgtggcgg ccaacaaggt
    49081 gcgcggcgtc cgcgccggcc tgtgccacga cacgtactcg gctcaccagg gcgtcgaaca
    49141 cgacgacatg aacgtgctct gcctgggggc gcgcgttatc gggcccgagg tggcgcagga
    49201 gttggtgcgc gccttcctcg gcgcgcgctt ttccggagcg gaacgccacg tccgccggct
    49261 gaaaaaggtg ctggcgctcg agtgacctcg cccatcctct acctccacgg cttcgcctcc
    49321 ggtccccttt cgagcaaagg agcttatttc gagcgccgct tt
//
LOCUS       DASREK010000173         8979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_86109, whole genome shotgun sequence.
ACCESSION   DASREK010000173 DASREK010000000
VERSION     DASREK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86109"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFA54_08870"
     CDS             <1..671
                     /locus_tag="VFA54_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HZS50957.1"
                     /translation="GWHHVLGREERIPPGNLCYGPGFDREEIKKVLENCKLRFRYLLT
                     AGEVVETAVAKLAENQIVAWYQGRMEFGPRALGNRSILASPLNPYSTENLNIYIKHRE
                     PFRKFAASVPAERAGEFFECGPNARFLATVSRVKPQYRQQFDGAVLRGDLIRVHTVHK
                     EDNPLYWELLQAAGRATGVPVVYNTSFNLFGEPLVCNPRDAVRSFYSSGIDSMFVGNF
                     FLEK"
     gene            837..1196
                     /locus_tag="VFA54_08875"
     CDS             837..1196
                     /locus_tag="VFA54_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50958.1"
                     /translation="MTKKLLRLGMAAALFAGICGAEQKTITGYLIDKACSSDAIKKGE
                     SMAKTHDIGCLTMDDCAKSGFGVYTTDGKFVSFDPAGNQQALAAVKSSGKKTDLRVTV
                     TGNVTGDTIKVSSIKVN"
     gene            complement(1270..2166)
                     /locus_tag="VFA54_08880"
     CDS             complement(1270..2166)
                     /locus_tag="VFA54_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS50959.1"
                     /translation="MTRREFHRTAALLPLAARAAPAPPPMRIIDPHVHVWTQDPRYPW
                     APQASKPPRREALPEELLQLMKANGVSHTVLIQVIYYTWDNRFARDTLRKYKGVFQGV
                     CRVNPESPSAPDDLSRLVKEDGFRGVRLSPSATAAGDWIRGPLMPPLWQRTQELKVPM
                     TLLLPTTRLPDAARLIERYPDLDVVIDHMADCPIDQPAELEKLLDLARFPRVYVKISH
                     TWSLSRQPYPFRDTHDQVHKLYDAFGPQRLMWGTDWPISERWCAYAQTLSVVRDEMKF
                     LNAEDKLWILGKTVERIWPFPA"
     gene            2360..2566
                     /locus_tag="VFA54_08885"
     CDS             2360..2566
                     /locus_tag="VFA54_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50960.1"
                     /translation="MSRRFWHSLASVLSGNAIYFGFWRYLPARAQHKPFEIDWGLAVD
                     FWICLAIYGLLARLKWFQQPPDKR"
     gene            complement(2672..3967)
                     /locus_tag="VFA54_08890"
     CDS             complement(2672..3967)
                     /locus_tag="VFA54_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS50961.1"
                     /translation="MERRTFILSAGTALAAMRAAGANDRVNVGVIGLRGRGRSHMSIF
                     GSQKDSTVAAVCDIDDAQTERAVQLVQKVQGTKPNTFRDLRKLYDDKSIDAVSIATCN
                     HWHALAAIWACQAGKDVYVEKPACHNIFEGRQMVAAARKYNRIMQVGHQSRTIQHKRK
                     AIEMLHQGVIGKIYMAKGLCFKRRPSIGHAPDGPVPPGVDYDTWLGPAPMRPFNPNRF
                     HYNWHWFWDTGNGDIGNQGVHEMDLARWGLNRKLPTKVTASGGKYIYNDDQETPNTLI
                     ATFDYGDAELVFEVRGILTGNEAGMPQVQGTNYIGDIYLGSDGYATLDDLAFRVYLGE
                     KRELAQEVKMMEAGPSDTVGHVRNFLDAVKSRKYTDLHCDIEEGRLSASLCHLANISY
                     RTGRKLTFDPVTETFGSDKEANSYLSRNYRAPFVVPERV"
     gene            complement(4215..4547)
                     /locus_tag="VFA54_08895"
     CDS             complement(4215..4547)
                     /locus_tag="VFA54_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZS50962.1"
                     /translation="MREQKDLLPPAQLLRNEDILDNGVRLTRMESQLLLWLRRHPGKC
                     LSRKFLLETIWGYKEGVRSRTLDVHIRRLRRKLGPLGHDCIKTVFRNGYSWCPSDHEL
                     LGGSSRFA"
     gene            complement(4614..5420)
                     /locus_tag="VFA54_08900"
     CDS             complement(4614..5420)
                     /locus_tag="VFA54_08900"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HZS50963.1"
                     /translation="MTCEQIDRFLDQQWSSGDRAVPQQVAEHLQQCEPCRQLFRLVRR
                     TAPDWQVPPESQARLTEAVLQSLEPVLPLGRRRFTLDLLGAFLVILLLGIAVAGIRSP
                     WVMSAGQFLTVTLVLAAGAIVLALSLARQVMPGSSHKVKPGVLLAAIAAAFAGSLLLL
                     FPWDMSGRFWFRGLLCFSFGAVLACVATVPLWMIVRRGALLSPALTGASTGLLAGVLS
                     AVVIHLGCPMATAPHLAIWHAAVPVFSAFAGFLVGHFSSHHPAGSCRPRA"
     gene            complement(5417..6004)
                     /locus_tag="VFA54_08905"
     CDS             complement(5417..6004)
                     /locus_tag="VFA54_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS50964.1"
                     /translation="MSSACGMLGVTSASAVERAGLEELMAGYQRADAEAAAELVRQLS
                     PVLFRFFASDLATRAFAEDLLQECWLRIHKSRHTYLPGEPLLPWVFAIARHTRVDAFQ
                     RRRRIESHELAIEGCDHCAAPPDAESGDLSRLLERLPESQREVILMLKVAGMTLEEVA
                     GSTSSTVGSVKQKAHRAYRKLRQILEQSASERARP"
     gene            6208..6981
                     /locus_tag="VFA54_08910"
     CDS             6208..6981
                     /locus_tag="VFA54_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HZS50965.1"
                     /translation="MPWPNAGVAPGRSPRGLMADWGGVDGCREGWVLAVVDAARRIVV
                     LHVLRTFAEVALETQNVSLTLADIPIGLPSGESARERLCDAIARKELGPRASTVFPVP
                     AREAVWADSYAEACRRNEKILGRKLSKQSWGICPKIREVDAVLRIAPKLQERIRETHP
                     ELCFRWLNRGKIIEDPKRSQAGRRIRRELLEQWTTNLETAMKQARRAHRTREATPDDL
                     LDAVAAAVAARLAGEGQVKSIPLAPERDAAGLRMEIVGI"
     gene            complement(7019..8059)
                     /locus_tag="VFA54_08915"
     CDS             complement(7019..8059)
                     /locus_tag="VFA54_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS50966.1"
                     /translation="MTVGKMLLAWGKVLIGNAPMLSIEITRECPLHCPGCYAYGDAHL
                     GGGTTLRQLNDLRGDALVHGVIGLVKQHGPIHVSLVGGEPLVRVRELNRILPALSGMG
                     VRTMVVTSGVIRVPVEWMSIPRVLVTVSVDGLPEHHDVRRAPATYERILRNIQGCKVN
                     IHWTVTAPMMERPGYLHEYLAFWDARPEVRRIWISSYTPQRGERSAEMLSAESRKELA
                     RQIPELRRRFPKLWINEGVAQAYVEPPPSPEACLFSRMSVNYSADLKTRVEPCIFGGD
                     PDCGQCGCAISTGLHWVGRLRLGPITVFRFAAASTAVGRLVARFRQRGRSELRRGAVR
                     SGSSPKFVQIAQ"
     gene            complement(8280..>8979)
                     /locus_tag="VFA54_08920"
     CDS             complement(8280..>8979)
                     /locus_tag="VFA54_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZS50967.1"
                     /translation="GVNAHPEFGRRVAARLAERTGIPFVSSPNFFFSMSTQDTAAELS
                     GQLNAAACGLMKISNDLRWMNSGPQAGLAEIALPDLQPGSSIMPGKVNPVIPEAMTMV
                     CAQVMGNHVAITVADQAGNFQLSVMLPVIAHNLLQSISLLANASRLLADKAIAGFKVN
                     ETHLTDLVDKNPILVTALNPVIGYELGAKIAKRAYAEGRKVKEIAAEMSNLAPAELDR
                     LLDPKRMTEGGAAK"
BASE COUNT         1787 a         2673 c         2876 g         1643 t
ORIGIN      
        1 acggatggca tcatgtgctg ggcagagagg agcgcatacc gccgggaaac ctgtgctacg
       61 ggccgggatt cgaccgcgag gagatcaaaa aagtcctgga aaactgcaag ctgcgcttcc
      121 gctatttgct gaccgccgga gaggtggtgg aaaccgccgt cgccaagctg gcggaaaatc
      181 agattgtggc ctggtaccag ggccgcatgg agttcgggcc gcgcgcgctc ggcaaccgga
      241 gtattctggc ttctccgctg aacccgtatt cgacggaaaa cctgaatatt tacatcaagc
      301 accgcgagcc gttccggaaa ttcgccgctt cggtgccggc cgagcgggcg ggtgaattct
      361 tcgaatgcgg gccgaacgcg cgttttctgg cgacggtaag ccgcgtgaaa ccgcaatacc
      421 ggcagcaatt cgacggcgcg gtgctgcgag gcgatctgat ccgggttcat acggtccaca
      481 aagaagacaa cccgctttat tgggagttgt tgcaggccgc cgggcgcgca accggagtgc
      541 cggtggtcta caacacgtcg ttcaacctct ttggcgagcc gctggtgtgc aatccccgag
      601 acgcggtgcg tagcttttat tcctcgggga tcgattccat gtttgtgggt aacttttttc
      661 tggaaaagtg agcaaagcaa cagtgaggga ctctttcagc ccggcttgcc ggaaggcttg
      721 tgcggccggc cctggtggcc ggccggcatc ctgaatccgg cgccggacca tcggaacggt
      781 tccgcgcggc gtggttcaga tgtccgcgcc gcaggcgtgc atggagaaaa gaaaatatga
      841 ccaaaaaact cttgcggtta ggaatggctg cggctctttt cgccggcatc tgtggggcgg
      901 agcagaaaac aatcaccggg tacctgattg ataaggcctg ctcgtcggat gcgatcaaga
      961 aaggcgagag catggccaaa acccatgaca tcggctgcct gaccatggac gattgcgcca
     1021 aatcgggctt cggcgtttac acaaccgacg ggaaatttgt atcgttcgac ccggccggca
     1081 accaacaggc gctggcggcg gtgaagtcgt ccgggaaaaa gaccgacttg cgagtcaccg
     1141 tgacgggcaa cgtgaccggc gacacgatca aggtgtcgag catcaaggtc aactaggctg
     1201 gggggcagcc cgcggcgccc ggccaacccg cctgcgcggc ttgcctgcgc gcgcccggcg
     1261 cgggcgattt caggcgggaa agggccagat gcgttcgacg gttttgccga gaatccagag
     1321 cttgtcttcg gcgttgagga atttcatttc atcgcgaacg acgctcaggg tttgcgcata
     1381 ggcgcaccag cgctcggaga tgggccagtc ggtgccccac atcaggcgct gcggcccgaa
     1441 ggcatcatag agcttgtgca cctggtcgtg agtatcgcgg aaggggtaag gctgcctgga
     1501 aagcgaccag gtgtgagaga ttttcacgta gacgcgcgga aagcgcgcga ggtcgagcag
     1561 tttttccagt tcggcgggct gatcgatggg gcagtcggcc atgtggtcga tcaccacatc
     1621 gagatcgggg tagcgctcga tgaggcgggc ggcgtcgggc agccgtgtag tcggcagcag
     1681 cagggtcatg gggaccttaa gttcctgggt gcgctgccac aagggaggca tcagaggccc
     1741 acggatccaa tctccggccg cagtggcgga ggggctcagc cggacccctc gaaagccatc
     1801 ctccttcacc aggcgcgaaa gatcgtccgg agccgaaggg ctttccgggt tgacgcggca
     1861 gacgccttga aacacgccct tgtactttcg cagcgtgtcg cgggcgaatc gattgtccca
     1921 ggtgtagtaa atcacttgaa tcagaaccgt atgggagacg ccgttggcct tcatcaactg
     1981 caacagctct tcgggcagcg cttcccggcg cgggggtttg ctcgcctggg gcgcccaagg
     2041 atagcgggga tcctgcgtcc aaacgtgaac gtgcggatcg atgatgcgca tcggcggcgg
     2101 agcgggcgcc gcgcgcgcgg ccagcggaag caaggccgcg gttcgatgaa actctcgccg
     2161 ggtcacagca tcacctcttc actcgcgttt gatttgatct ggacgttggg gaccggcctt
     2221 gacagaggcc ggagaatcag ccgacaatca gccttctcca ggaaacacac cgctcgttcc
     2281 ctggaccgac tggctgcgct gctgctgccg ggatccaagg cgccccggaa aaaaacaacg
     2341 ccgcagtctt ccgttcccga tgtcgcgccg cttctggcac agtctcgcat ccgtactatc
     2401 cggcaatgcc atttatttcg gcttctggcg ataccttccg gcccgcgcgc aacacaagcc
     2461 gttcgagatc gattggggac tggcggtaga cttctggatt tgcctcgcca tatacgggct
     2521 gctcgcgcgc ctgaagtggt ttcagcagcc gcccgataaa cgctgacgcg ccgcgaacgc
     2581 tccgttcccg acgcaggagg gagcgatccg gcggcttccc cagacggatc cagacaaagc
     2641 caggttccag cgcgagaaaa accaaagcag cttacactct ctcggggacc acgaacgggg
     2701 cgcgataatt tcgcgagaga taggaattcg cttccttgtc gctgccgaag gtttccgtca
     2761 ccgggtcgaa cgtgagtttt ctgccggtgc gataggaaat attcgccagg tggcagagcg
     2821 atgccgaaag ccttccttct tcgatgtcac agtgcaaatc ggtatatttg cggctcttga
     2881 cggcgtcgag aaagttccgc acgtggccga ccgtgtcgga ggggccggcc tccatcattt
     2941 tcacttcctg cgccagctcg cgcttctcgc caagatagac gcggaaagcc aggtcgtcga
     3001 gggtggcgta cccgtcgctg ccgaggtaaa tatcgccaat gtagttcgtg ccttgcactt
     3061 ggggcatacc ggcctcgttg ccggtgagga tgccacgcac ttcgaagacc agttccgcat
     3121 cgccataatc gaacgtggca atcagggtat tgggagtctc ctgatcgtcg ttgtaaatgt
     3181 acttgccgcc cgaggccgta actttagtag gcagcttccg gttcaggccc cagcgggcga
     3241 gatccatctc gtggacgccc tgattgccga tgtcgccgtt gccggtgtcc caaaaccaat
     3301 gccagttgta atgaaaccgg ttgggattga agggacgcat gggcgccggc ccgagccagg
     3361 tgtcgtaatc gacgccgggc ggcaccgggc cgtccggcgc atggccgatc gaaggacgcc
     3421 gcttgaaaca cagccccttg gccatataga tctttccgat cacgccctgg tgcagcatct
     3481 caatcgcttt gcgcttgtgc tggatggtgc ggctctggtg gccgacctgc atgatccggt
     3541 tgtacttgcg ggcggcagcc accatttgac ggccttcaaa aatgttatgg cacgccggct
     3601 tctcgacata gacgtctttc cctgcctggc aggcccagat ggccgccagc gcatgccagt
     3661 gattgcaggt ggcgatggag acggcatcga tgctcttatc gtcgtaaagt ttgcggagat
     3721 cgcggaacgt gttgggcttc gtgccttgca ctttctgcac aagctgcacc gcgcgctctg
     3781 tttgggcatc gtcgatgtcg caaacggcgg cgacagtcga atccttttgg ctcccgaaga
     3841 tgctcatatg cgagcgcccg cgcccgcgca atccgatcac gccgacgttt acgcgatcat
     3901 tggctccggc ggcgcgcatc gcggccaggg cggttccagc ggaaagaatg aacgttcgac
     3961 gctccatatc ggctccccct tcttctccag acacgcctaa aataccacga atccgaaggg
     4021 acgatgatgt gaaaagaagg gcggtcttgt tcctgcttgc gtccaagacc gccccggcta
     4081 ggggaagctg tacggtatgt tgtgcgcccg gcggccggct cacggcttca cagtccgacg
     4141 ggctttcgag cgggcgagcc ggccgaggca ggcgcgaatc gctccgctga ggccggcgcc
     4201 gccggggttg gcgatcacgc aaaacgggag gatccgccaa gcagctcatg atcgctcggg
     4261 caccaggagt atccgttgcg aaagaccgtc ttgatgcaat catgacccag cggtccaagc
     4321 tttctgcgaa gccgccggat atgcacgtcg agggtgcgcg aacggacgcc ctccttgtaa
     4381 ccccaaatag tttccaacaa gaatttgcgc gatagacatt tcccgggatg gcgccggagc
     4441 caaaggagca actgggactc cattcgggtg aggcggacgc cgttgtcgag aatatcctcg
     4501 ttccggagca gctgggctgg tggcagcaag tctttttgtt cccgcatagt gcattgttct
     4561 cgcatagtgc gttaagagtc ctctaagaga ttgccggggg aagcgaaaaa aagttacgct
     4621 cgggggcggc aggagccggc agggtgatgg cttgagaaat gtcccaccag aaagccggca
     4681 aaagcgctga agacggggac agcggcatgc cagatggcca ggtgcggggc cgtcgccata
     4741 ggacagccga ggtgaatgac gacagcgctc agcactccgg ccagcagccc cgtgctcgcg
     4801 ccggtgagag caggcgataa cagagcgccg cgccgtacga tcatccacag cgggaccgtt
     4861 gccacacagg ccagcacggc cccaaacgaa aaacaaagca gcccccggaa ccagaaccgg
     4921 ccggacatat cccagggaaa aagcagcagc agggagccgg caaatgcggc ggcaatggct
     4981 gccagcagca ctcccggctt taccttgtgt gaagatccgg gcattacttg gcgcgccagg
     5041 gataaagcca gcacgatcgc gccggccgcc agaaccaggg tcacggtcaa gaactgcccc
     5101 gcgctcatca cccacggcga gcgaataccc gccacagcaa tgccgaggag gagaatgacc
     5161 aggaaagctc cgagcaagtc gagggtgaat cgccggcgcc cgagcggcaa aaccggctcg
     5221 agcgattgca gcaccgcttc cgtcagccgc gcttgactct ccggcgggac ctgccagtcg
     5281 ggcgcagtgc gccggaccag ccggaataac tgcctgcaag gctcgcattg ttgcaggtgc
     5341 tccgctacct gctggggaac ggcccgatcg ccgctgctcc actgctgatc gagaaaccgg
     5401 tcaatctgtt cgcaggtcat gggcgtgctc gctcggaagc ggattgttcg aggatctgac
     5461 gcagcttccg gtaggcgcga tgagccttct gcttcacgga cccgacggta gatgacgtcg
     5521 agcctgcaac ttcttccagg gtcataccgg cgactttcag catgaggatc acctcccgct
     5581 ggctttcggg aaggcgctcg agcaggcggc tcaagtcgcc ggattcggca tccggaggcg
     5641 ccgcgcagtg atcgcagcct tcgatcgcca gctcatgcga ttcgatgcgg cgccggcgct
     5701 ggaatgcgtc aacccgtgta tgccgggcga tggcgaaaac ccacggcaac aagggctcgc
     5761 ccggaaggta ggtgtggcgc gacttgtgaa tgcgaagcca gcattcctgc aagaggtctt
     5821 cggcaaaggc ccgcgtggcc aggtcggagg caaaaaaccg gaacaggacc ggggagagtt
     5881 ggcggaccag ttcggccgcc gcttctgcat cggcccgctg gtagccggcc atgagttcct
     5941 ccaagccggc tctttccacc gcagatgcgg atgtgactcc tagcatgccg caagcgctac
     6001 tcaagatgac tccagcattt cagaaggccc ggcatttcac aatgctatgc agccggcccg
     6061 tctaaaaagt tacgtcctga ttgtattttc ctgaccggct cccgggcgag acgggctgcc
     6121 ggcccgaaag gatgccggac tcggcttgaa aacgtttctc catcgcgggg gctcgagggt
     6181 tgtctatcat aggatgcctg catacggatg ccttggccta acgccggagt ggctcccggc
     6241 aggtcgccgc gaggtttgat ggccgattgg ggaggagtgg acggctgccg ggagggctgg
     6301 gtcctcgccg tagtcgatgc ggcgcggcgt atcgttgtgc tccacgtgct gcggaccttc
     6361 gcagaggtgg cgctggaaac acaaaacgtg agtctcacgc tggcggacat ccctattggg
     6421 ttgccatcgg gcgaatctgc ccgagagcgt ttgtgcgacg ccatcgcccg aaaagagctc
     6481 ggcccgcgcg cctccacggt atttcccgtg cccgcgcgcg aggctgtctg ggccgatagc
     6541 tacgcagaag cttgccgacg caatgagaag attctcggac gcaaactcag caagcaatct
     6601 tggggaatat gcccgaagat ccgcgaggtg gacgcggtgc tgcgaatcgc gccgaagctg
     6661 caagagcgga tccgcgaaac gcatcccgag ttgtgttttc ggtggctgaa ccgggggaag
     6721 attatcgagg acccgaagag atcacaggcg gggcggcgaa tccggcgcga gctgctcgag
     6781 caatggacca caaacctcga gaccgccatg aagcaagccc gccgcgcaca tcgcacgcgg
     6841 gaagcgacgc cggatgacct gctggatgcg gtcgcagcgg cggtagcggc gcggctggcg
     6901 ggtgaaggac aggtcaagag cattcccctg gcgcccgagc gcgacgcggc cgggctgagg
     6961 atggaaattg tcggcatctg aaaggctccc tcctcgattg aaaccgtgga tttgccgttt
     7021 attgcgcgat ctgcacaaat ttcggcgagc taccggatcg caccgcgccg cggcggagct
     7081 cgctgcggcc gcgctggcgg aagcgggcca cgaggcgtcc gacggccgtg gaggcggccg
     7141 caaaacggaa cacggtgatc ggccccaggc gcaagcgtcc gacccagtgc aggccggtgc
     7201 tgatggcgca gccgcactgc ccgcaatccg ggtctccgcc gaagatgcat ggctcgactc
     7261 tggttttcaa atcggcggaa taattcacag acatccggga aaacaggcag gcctcggggc
     7321 ttgggggcgg ctcgacgtag gcttgggcga ctccttcgtt aatccagagc ttcgggaaac
     7381 gccgccgcag ttcgggtatt tgccgtgcca gttctttgcg ggattccgcg ctgagcattt
     7441 cggcgctgcg ctcgcctctc tgcggcgtgt aggaactgat ccagatgcgc cggacctcgg
     7501 ggcgcgcgtc ccaaaacgcg agatactcgt gtaagtagcc gggcctctcc atcatcggcg
     7561 cggtaacggt ccaatggata ttgaccttgc atccttggat attgcgcagg atgcgctcat
     7621 aggtcgccgg agcgcgccgg acgtcatgat gttccggcaa gccatcgacg gaaacggtca
     7681 ccaaaaccct gggtatggac atccactcga cgggaacgcg aatcacgccg ctggtgacaa
     7741 ccatggtgcg gacgcccatg ccgctcaacg ccggcagaat ccggttcaac tcgcgcacgc
     7801 gtaccagggg ttcgccgccg acgagcgaaa cgtgaatcgg gccgtgctgt ttcaccagcc
     7861 cgatcacccc gtgcaccagc gcatcgcccc tgagatcatt gagctggcgc agcgttgtac
     7921 ccccgcccag atgcgcatct ccgtaggcat aacaacccgg gcaatgcagc gggcattccc
     7981 gcgtgatctc gatcgaaagc atgggggcgt tgccaatcag caccttgccc caggcgagca
     8041 gcatcttccc gacagtcatc cgcagccccc tttccgcaaa gcggcttgca ccctttcaat
     8101 ctgaaatgct aatggttaga cagctacaaa ggcggagggg ttgctggaat caacaaggac
     8161 tcgcacgtga aaatgtggga tgcccccggc agactcgcct gagttgggcg agatttcaca
     8221 actcggcgaa gacaaggcca gcggccgccg actcccgggc agcgcaagtt gtattggggc
     8281 tatttggcgg cgccgccttc ggtcatccgc ttgggatcca acaggcgatc gagctcggcg
     8341 ggcgcgagat tgctcatttc cgccgcaatc tctttgacct tccggccctc ggcgtaggct
     8401 cgcttggcaa tcttggcgcc caattcatag ccgatcacgg gattaagcgc cgttaccaga
     8461 atcggatttt tatccaccag atccgtcaga tgcgtctcat tgaccttaaa gccggcaatg
     8521 gccttatcgg caagcaggcg cgaggcgttg gccagcagag agatgctttg cagcaggttg
     8581 tgcgcaatca caggcagcat gacgcttagc tggaagttgc cggcctggtc cgctacggtg
     8641 atcgccacgt ggttgcccat cacctgggcg caaaccatgg tcattgcttc cgggattacg
     8701 ggattgacct tgccgggcat gatactcgag ccgggctgca aatccggcag cgcgatttcg
     8761 gccagaccgg cttgcggccc gctgttcatc caccgcaggt cgttggaaat tttcatcaga
     8821 ccgcaggcag cggcattgag ctggccgctg agctcggccg cggtgtcttg cgtgctcata
     8881 ctaaaaaaga aattcgggct tgaaacgaaa ggaatgcccg tgcgttcggc gagcctggcg
     8941 gcgacgcggc ggccgaactc cgggtgcgcg ttgacgccg
//
LOCUS       DASREK010000174        29635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_418206, whole genome shotgun sequence.
ACCESSION   DASREK010000174 DASREK010000000
VERSION     DASREK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 29635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29635
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418206"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFA54_08925"
     CDS             <1..301
                     /locus_tag="VFA54_08925"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZS50968.1"
                     /translation="LHNYKVRVTLTGANLDSTGLLCDFVEVKRMMDAIIERLDHRFIN
                     EIPPFDELNPSAENLARYFHQQLALGLREKPPAVPVRIGEVQVWETDSTTATYRE"
     gene            303..941
                     /locus_tag="VFA54_08930"
     CDS             303..941
                     /locus_tag="VFA54_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS50969.1"
                     /translation="MQVTEIFHSIQGESTFTGLPCTFVRLTGCNLRCVWCDTEYAFYG
                     GEKMTVEQVLEKVRAFGGRLVEITGGEPLLQKEVYPLMERLLDEGYQVLLETSGERSI
                     ARVPRDVVKIVDVKCPDSGEGGAFDLSNLDAIAPHDQVKFVIASRRDYEFARDFTREH
                     DLCRRAAAVIFSPVYGKIDLERLAAWILEDRLEVRFGYQLHKLIWGADARGV"
     gene            1062..2222
                     /locus_tag="VFA54_08935"
     CDS             1062..2222
                     /locus_tag="VFA54_08935"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZS50970.1"
                     /translation="MATEAVLAATLVEPGRYELREYPLPEPAPGCVLVKMELSGICGT
                     DKHTYQGYTTQYSGAGEGKKIRFPIIQGHENVGTIAAIGGSGGCSDFEGAPLRAGDRV
                     VVGANVSCGRCYYCTHDFPYYFCQNMTDYGNNLCAADPPHIFGGWSQYIYIVPGSFLV
                     RVPDELPSEIAVLTEVMAVTVGLDRAKQMSAFPDEPFLFDDTVAVLGVGPLGMCFLLK
                     ARMLGAGTIIAIDLSNYRLDFARRLGADHLINAAATTLAERLEIVRDLTHGRGADVVI
                     ECAGVPQAIPDALEMLRLGGLLVEAGNFSDLGEVRISPHRHLCSKSVRVLGVGGEEPA
                     SYGPSMRQMARYMRFYPLQELVSHRFPLREVESAVHKSMAADSMKVVIEPWA"
     gene            2339..3589
                     /locus_tag="VFA54_08940"
     CDS             2339..3589
                     /locus_tag="VFA54_08940"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZS50971.1"
                     /translation="MSAWPVYTDAEVAALQDVLASRNWGGQPFPGKHSEAFAKNFAEL
                     HTVKYAQCVNTGTVAIQAALKAIDIQPGDEVLVPAYTWEGTVGPVLLVNAVPVFVDVD
                     PETYCIDPNQIEEFISPRTRAVLPVHLGMRFADLDAIMRIAQKHGLKVIEDCAHVHGG
                     LWRGKGAGSIGDLGAFSFQSSKLITCGEGGAVVTNNLEYFERVQSYINAGRASVTDKF
                     RHRLIGFNYRLGEFQAAVLGPQLERLPEQARIRQANMDYFESRLRGAPGLGFLRPEPR
                     ITRPAPYAYVLKYFAEQAKQIPRAAFVAALQLEGIPCDGLFYEPVYRSSLFPVNPCEY
                     PALSWGRPAPLELRNMYRCPASERAAYQEAVWFPHHLFLGSQADTDTIVAAVHKVLEN
                     IEELRGVEHPAIEAQNLSRADRES"
     gene            3570..4676
                     /locus_tag="VFA54_08945"
     CDS             3570..4676
                     /locus_tag="VFA54_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS50972.1"
                     /translation="MLIGKADEERRSRLPGPDHRSPSLLTGGNVKPVGYAVVGLGAIA
                     QQAVLPAFAHSNKARLAAVVSGDKEKAEQLAGKFRAPHYYTYEAYAECLKNPAVEAVY
                     IATPPGRHETFTLDAARAGKHVLCEKPLAATVAEGRRMVHACRKNGVRLMTAYRKYFE
                     PASAALKKMIAEGRLGRVDIIHTAFTEFHRAGGSAPPWLLQRKLAGGGPLMDLGVYCV
                     NTGRWLVDEDPVQAAAFSWRHDRKRFRQVEQGIAFRLNFASGLVLQGSSSYGSALASF
                     VQIHGEKGWAALSPAFAFEQERRLAGSIAGRWFEMRFGVIDEFALELDAFAECIRRNR
                     PAEPDGEQGMRDLIIIDAIYRSARQGRPVRIRYS"
     gene            4737..5756
                     /locus_tag="VFA54_08950"
     CDS             4737..5756
                     /locus_tag="VFA54_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS50973.1"
                     /translation="MAKLGLGVVGVGTMGRRHAENIRRLVPEARLIAVADVDLARARQ
                     VAGELEIEHYYGSVEALVERKDIQAVAVATPAKFHGAAIRSCAEAGKDVFCEKPLTLT
                     VQEADEILALAAKAGIRVQPGHVRRYDPPNVKAKKRIEAGEIGDPVIFKSLARDPAPP
                     PVSYMQSGWNGMFFHDSTVHEFDLGRWLMDDEIAELHAYGAVLVFPEIAQFNDIDTAL
                     VNMKFSRGALGSVENYMQACYGYDVRTEIVGSKGAILMGSLQQTSEVVLTASGAKTDI
                     VDHFLVQFADAYLEEMRDFVRTILADLKPKVTAFDGRQAVAAAAAAERSYREGLPVKL
                     DRQPA"
     gene            complement(5942..6637)
                     /locus_tag="VFA54_08955"
     CDS             complement(5942..6637)
                     /locus_tag="VFA54_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZS50974.1"
                     /translation="MRDWLLIDADDTLWENNIYFEQAFEQFVAFLDHSRLSAAEIREV
                     LDEIEAANIKLHGYGSANFGRNLQECFRRLAERPYGDGDLAMVLGFAGAILERPMEVI
                     EGVEDTLRYLAGRKRLLLFTKGHPEEQKLKLGRSGLGRYFEDCEVVKEKDAHNYRRVV
                     ETKNLDPGATWMVGNSPKSDINPALEAGLNAVLVPHPHTWSLEKQEIVSPGERRAGRL
                     LIVERFADLQRYF"
     gene            6789..7580
                     /locus_tag="VFA54_08960"
     CDS             6789..7580
                     /locus_tag="VFA54_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZS50975.1"
                     /translation="MHVKVLFFGMLKDLVGRAEEQIEVPEGARLSNIFEQYCRRFPRL
                     AEMAGSIVLARNQEFAGPEARIGEGDEIALLPPVSGGSSPAPEAASTANRTAGPDSGI
                     LAEFEDELGNYFAITRVPIDARALAARLVRGCDGSVITFEGIVRDNSKGRATLYLEYD
                     GYVPLAIKKMREIASDLLAAHGADRIAMVHRLGRMEIGETSVVIVVTSAHRKPAYEVS
                     REAIDRLKRLVPIWKKEHFADGEVWVEGEWDASVPRLEGSSRCSG"
     gene            7568..8587
                     /locus_tag="VFA54_08965"
     CDS             7568..8587
                     /locus_tag="VFA54_08965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS50976.1"
                     /translation="MFRLSIFAACLLACLCAQEQPGRFNVEVKLVTVLATVKNNQGVP
                     TGDLEREDFTLLAAGVPQKIAVFERQTDRPLSVALLFDASPSVAKELRFEQEAAVRFI
                     RSLLGSGANPADRIAVYKFSDFVDEVQGFTNSVQRLERAALSIRTHGGTSLYDALYLA
                     AQGLQNREGRKVIVVITDGDDTTSDIKFSQALEAVQLADAVVYSIIVVPITNDAGRNL
                     GGENALKTMSAATGGLAFLQHAGQDLDSNFRQIERDLRLQYLIGFYPQGVPAGPDRFH
                     RIELKVKRPGMKALARTGYFSGPEEEPVTSYAPAVVVDGSPPRERKKEAPAARGGKSS
                     PRSIP"
     gene            8584..8874
                     /locus_tag="VFA54_08970"
     CDS             8584..8874
                     /locus_tag="VFA54_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50977.1"
                     /translation="MKGPFKPRFNRPPHGGGQGGGGSKSHKAPDQTMQEVQYLKELIE
                     KQATVCIKLRNNEEVRGTIEYYDNAFIRVTRASAPNLFIFKHEIKYLYEEAK"
     gene            8946..9776
                     /locus_tag="VFA54_08975"
     CDS             8946..9776
                     /locus_tag="VFA54_08975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HZS50978.1"
                     /translation="MSSSYLSAASEIAREAGALLARYFERRVAVEYKGEFDVVTEADR
                     ASESLIVSRLRTRFPHHSIVAEEGSGIERSSEFTWYVDPLDGTTNFAHGFPMFAVTMG
                     LEQAGELVAAVTYDPLRDEFFSAEKGGGAYLNSRRLQVSRVSRLSESLLATGFPSRKR
                     HGDVNIYFYHQAAMLTHGVRRAGSAALDLAYVAAGRLDGFWEFRLNAWDIAAGLLLVR
                     EAGGCYSDMHGAEHRFDSPELLASNGLIHRELLSLFGDIFANRIRVPLPALAGAVPPS
                     "
     gene            9904..11208
                     /locus_tag="VFA54_08980"
     CDS             9904..11208
                     /locus_tag="VFA54_08980"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZS50979.1"
                     /translation="MRTVDLIRKKRDGEELTEKEIDYLIQNYTRGEIPDYQMAAFLMA
                     VYYSNMSESEVTALTLAMLNSGETLDLSDLPGAKISKHSTGGVGDKTSLIVGPIAAAA
                     GVAVPQITGRGLGHTGGTLDKLESIPGFRTNLSIEEFKTVLKEHKLSFIGQTDRIAPA
                     DKKMYALRDATASVESLPLIAASIMSKKLAEGIDGLILDVKVGSGAFMKKQIEARKLA
                     QLMVTIGRRMGKRVQALLTDMDQPLGYAVGNALEVMEVSQTLRNEGPADLTQLSVELA
                     ARMIHLANPSQPIDSARERAGELLASGEALAKLQQVIAAQGGNPEALNQFELLPNATG
                     AREIISPRAGYVTRINAEDVGQASVLLGAGRDTMEKPIDPAVGIILERKVGEKVPAGG
                     RLCTLYYTDETHLEEAAQLVEDAFHISANAAEPRELVLEVVQ"
     gene            11305..12528
                     /locus_tag="VFA54_08985"
     CDS             11305..12528
                     /locus_tag="VFA54_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HZS50980.1"
                     /translation="MSRFTGLLGLAAIIGGAYAFSSRRKSIPVRVVAWGLGLQFALAL
                     LVLRTPFGLLFQWIGAGVNHMLDFAQAGSEFVFGPLAKANGPAGVVFAFHVLPVIIFV
                     ASFFAVLYYLGVMQAIVKGIAIAMQRVMGSSGAESLDVAASIFMGQTEAPLTIRPFLP
                     GLTESELLTVMAAGMAHVSGALIVAYVDIAHVEAKHLLTAVIMTAPATIMLSKMLIPE
                     TEQPETAGGVELHVEKLDVNVIDAAARGATEGLHLALNVAAMLVAFIALIALGNGLLG
                     GLHSIRGFGWAPDSLQRLFGWVFAPVAWIMGVSWRDAPAVGNLLGTRMVLNEFVSYVL
                     LGPMKPQLEPKSFVIATFALCGFANFSSIAIQVGGIGALAPTRRHDLARLGLRAMITG
                     TLANFMSAAIAGILL"
     gene            12597..13478
                     /locus_tag="VFA54_08990"
     CDS             12597..13478
                     /locus_tag="VFA54_08990"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HZS50981.1"
                     /translation="MRSLLLPRAHPAKARKGSLNYLQVEKACRFLISHLEVVPAAAVV
                     LGSGLEAFAGELSNPVEISYGKIPHWPRATVAGHAGRLVGGVIGGKPVAVLAGRAHLY
                     EGYSALEITFPIRVLGLAGIKTLVLTNAAGGINPQFHPPQLVLITDHINLQGANPLIG
                     VSDERFGPRFPDLTEVYPKRLRDLARQCAREIGRAHGLELPEGVYAAVSGPSYETPAE
                     IRFLRTIGADMVGMSTVPEAIVAAQMGIPVLGISMISNMAAGMLPQKISHQEVLEAGR
                     RMSGTLITLLKKLIPRL"
     gene            13475..13870
                     /locus_tag="VFA54_08995"
     CDS             13475..13870
                     /locus_tag="VFA54_08995"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZS50982.1"
                     /translation="MNPQELIQSAVTAREHARAPFSHFRVGAAIETRSGAVYTGCNIE
                     NATYGLTVCAERVAVWKALSEGEAEFIRIAIAADTETLTPPCGACRQILWEYCGDIEI
                     ILANLGGKTETMPLAALLPRPFDAAFLEE"
     gene            13848..14534
                     /locus_tag="VFA54_09000"
     CDS             13848..14534
                     /locus_tag="VFA54_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HZS50983.1"
                     /translation="MLHFWKNSAVLEDVRRNRERGGHSPPAAPDRRLGILPSAFNPVT
                     VAHVELAQQAMLQYRLDEVLLTLPRAFPHKGYVGPTFEERIEMLLAAAAEEPRLSAGS
                     TDGGLFIEIVRECREVYGCAAEFFIVCGSDAAERAIAWDYARQAPAAGPFAEQIKEFQ
                     MLVAPRGGPWTVPPEYAGRIHLIAWPPELDLCSSSAVRAAIAQGRPWEHLVPPPVAAI
                     IRRRSLYSNT"
     gene            14634..14828
                     /locus_tag="VFA54_09005"
     CDS             14634..14828
                     /locus_tag="VFA54_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS50984.1"
                     /translation="MTTTAAEVYCDACHLAIGLQKHAICKKRRKGNPIEYVYYHNRDA
                     DDCWGKQLKALMRKYNGKAR"
     gene            complement(14912..17101)
                     /locus_tag="VFA54_09010"
     CDS             complement(14912..17101)
                     /locus_tag="VFA54_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HZS50985.1"
                     /translation="MNWNRNLWASEIPRHPARRKPNHYAEMLRAAWENRDQLPYAWRI
                     LNHGVCDGCALGTAGMKDWTMEGIHLCMVRLELMRLNTAPALDPGRLADVSSIEHLNS
                     QQLRGLGRLPEPMIRRRKEKGFRAISWEEAYDIAAEKIRGTRPERFAVYLTSRGILNE
                     HYYAAQKAARAIGTNHVDNSARLCHAASTTAMKKTLGYGATTCSYRDWIGADLIVFFG
                     SNTPNNQPVTTKYLYYARQAGTRVAIVNPYFEPGLQSYWIPSIFESAVFGTKMADDWF
                     AVDTGGDLAFLTGVFRILNTEGWIDTDFIARHTTGFEAAKTCAEQQDWELLERESGTT
                     REQMRRFAGMLHQARNGIFVWSMGLTQHAHGVQTIEMLVNVALARGWVGREHTGLMPI
                     RGHSGVQGGAEVGCVPGLDDQQRARFEQVWGFVLPAFPGLTAAEMVNAACQGRIDAFW
                     TTGGNFLETLPGPSTTAQALENIPTRIHQDVVLSSMMLLPPKDTVILFPATTRYESPG
                     GGTETTTERRIIFSPEIPGRRIGSAKPEWEVFGEVAARVRPNLADRVRFHSVAAIREE
                     ISRAVPLYSGIERLAKEGDNFQWGGERLFANGQFATPDGKAHFSVVSLQEHRRPEGMF
                     YVSTRRGKQFNSMVQRAADPLTGASRDSILISGEDARSLGIREGDLIRLSSASGSYVG
                     RAKIDKVKPGNLEVHWPEGNCLLSREVIDPSSREPDYNAAVRVEKVG"
     gene            complement(17233..18813)
                     /locus_tag="VFA54_09015"
     CDS             complement(17233..18813)
                     /locus_tag="VFA54_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZS50986.1"
                     /translation="MATEAVGAPATVAAPDVNPWIIAISVMLSTFMEVLDTTVVNVSL
                     PHIAGNLSASTDEATWVLTSYLVANAIILPITGWLANYFGRKRLLMAAVAGFTTASFM
                     CGLAPNLATLIFFRVVQGATGGTLQPISQAVLLEAFPPEDRGKAMGFWGLGIVVAPML
                     GPVLGGWLTDSYSWRWVFYINIPVGVASILMTKMFIFDPPYIRRPKTGIDYWGIGMLA
                     VGIGALQIVLDKGEEKDWFSTAWITELVVLSASVLIVFIIYELRARSPVVNLRVLKER
                     TYGTGVLLMTILGFGLYGSLVVLPLLLQELLGYPALKAGVTMFPRGLGSFIAMPVIGI
                     LVAKFDPRKLLALGFLGAGFTLIQLSWLNLNAGFWDFFWPQFLQGLSLGLLFVPLTTV
                     TMDPIPKEQMGNATSLFNLMRNVGGSVGIAMATTLNTRSTQRFTNVFGANVTPGSLQA
                     HNVFQALQLAFISRGFDPVTAAHKAQGALFGLVQQQAAVLAYLNTFRLLGLIFLLVIP
                     LLLLLRKPAHQGRGLPAH"
     gene            complement(18823..19437)
                     /locus_tag="VFA54_09020"
     CDS             complement(18823..19437)
                     /locus_tag="VFA54_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HZS50987.1"
                     /translation="MIRVITISREYGSGGGAVARMLAERLKWKLVDDALITEIAKKAQ
                     VNPAIARRYDECVDPWFHRLMKALWQGGFVGAASRVEGELFDAETMARLWSRVIEETG
                     AMGDCVIVGRGGQCILQNREDAFHVSIYAPLEERVRRLREQLPPEADPEAAAIESDRR
                     RAAYIRRYFGQDWTNRHLYDLAVCSSIGLERVVETILCAAGLKA"
     gene            complement(19448..20776)
                     /locus_tag="VFA54_09025"
     CDS             complement(19448..20776)
                     /locus_tag="VFA54_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZS50988.1"
                     /translation="MADAIQTEQRQQASGGPQAQTQTQLGQPERSWTPRRASFFRRRP
                     LAKWALLAAIILVAAGAYWTWRYYSSRETTDDAQVDAHMAPISARVGGPIIAVNVADN
                     QHVEAGAVLAQIDPSDYQVALERARADLAAAEAAARAAQTGVPITATTTESTLSSAEA
                     RLLSANAALSAAEKQVAAANAQLNVMQAKLREAEANHAKAAQDLVRYKELVAKDEIPR
                     QQFDAAVAAEAATGAAVESARADVRRAEQGILVAESQVAQARGTVAEAKAGVRAAQTA
                     PQQVASSQAQYRNAEARIGQARAAVNLAELNLQYTIIRAPIAGAVGNKTVQVGQVVQP
                     GQALMAVVPLEDIYVTANFKETQLHSMRPGQPAIVSVDTYGGRKYSGRVQSVSPATGE
                     KFSLLPPENATGNYVKVVQRIPVRIELDKGQDPDHLLRPGMSVEVTVIVK"
     gene            complement(20776..22233)
                     /locus_tag="VFA54_09030"
     CDS             complement(20776..22233)
                     /locus_tag="VFA54_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS50989.1"
                     /translation="MKAALIALGPVCMWLAAAGQALSQVPQLPPQTAAPVPQTPGVTF
                     SQQPPNPFLGSASMEKPVPGVLALSLEEAVNRGLRFNLGVLLNQQNTQFALGEKYRAR
                     SALLPNLRADIAGTDQQINLKALGFPGGFPGVPSIVGPFTVLDARAYVSQDVLNFNAL
                     HNLRSATQNLRAARYSYSDARDIVALGVAGLYLQASAGLARIDAAEAQVKTAQALYDQ
                     AVDKRGAGVVAGIEVTRAQVELQAQQQRLIFFQNEYEKQKLNLARAIGLPLGQQFELS
                     TRIPYTPPPSVSYEQALDQAYRSRADYQNALALLRSAEEARKAAAAQHYPTLSADANY
                     GTIGPGLTDSHGTFSVSGTLSVPVFRGGRIRGEVLQAEALLRQRRSESEDLRGRIDYE
                     IRAAFLDLRANSEQVRVAESALKLANQQLAQSRDRFAAGVADNIEVVQAQEAVATANE
                     NYISSLYSYNLAKASLARAIGAAEKTIIQFLEGTP"
     gene            22641..23759
                     /locus_tag="VFA54_09035"
     CDS             22641..23759
                     /locus_tag="VFA54_09035"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZS50990.1"
                     /translation="MRLRSVSRSRIRPRGFVLITVAITIVALLGIMGMCIDLSRMYIV
                     RNELQAYADAASLAAARHLDGTSLGISNATAIAKNYPDSYNFSTKAPDSVSVAFATDP
                     NGPYVSNPANPQGIKYVQVVANGSVTLYFLPAFAKTAPLSMLWTIGGNQSMTASAASG
                     QFLKSTAFSTGLLPYSPYAHNPNDPNFGFTVGEQYTLRWPPPGQQKKGSCPGDVGFPD
                     PSNSASQRGFIDIGWSQGSGSASFIREAIISGVQSKPLNVGDILNMVTGNKGTESDAL
                     QERYNEDTDTTSDYRTYLTDLQNGTANGRRLGIVPINDPSQNDKVLGFALVLLPANPC
                     GSKSTDPCCAVYLGNNPVLPALPGGATTPGAYQLKLNK"
     gene            23916..25067
                     /locus_tag="VFA54_09040"
     CDS             23916..25067
                     /locus_tag="VFA54_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZS50991.1"
                     /translation="MRWNISSCSRAKSGFVLITVAITIVALLGILGMCVDLSRMYITK
                     NELQAYADAASLAAAHHLDGTSLGITNATTIAKNYPDAYNFATTKPDSVSVAFASDPA
                     GPYVTNPANPQGIKYVQVTANGSVTLYFLPAFAKTAPLGMLWSIGGSQSMIAIATSGQ
                     FLKSNAFNTGLLPYSPYVHDPTDTTNYGFVPGRSYTLRWPPPGQENKNNACPAEVTQN
                     FSDPSSSNSQRGFIDIGWPAGNGNGGPGGGSAFIREAIISNVQSKPLGLGDDVNFVSG
                     NRGTESDALQERYTEDIDTTADWQTYMTDLNNGTANGRRLGIVPINDPNSSPAGKVVA
                     FGLFLLQNKPCGNSSVAPCCATFIGMNPILPGGTAGTSTPGVYQLKLFK"
     gene            complement(25119..25712)
                     /locus_tag="VFA54_09045"
     CDS             complement(25119..25712)
                     /locus_tag="VFA54_09045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZS50992.1"
                     /translation="MNRRQVLKGAITGAGAAVLRAAPAPPQPLPQVEQAGQGRNETSL
                     DRGAAGAGPWQPRVFDAHQNDTVVALTELIIPATDTPGAKEAQVNRFIDLMLSESSPE
                     ARRTFLQGLAWLDGYALRQNGKPFVRCSAAQQVELLRSLDPAANPPAELHTGVAFFQD
                     IKRRTAAGYYTSKIGYDELNKGGRVPASFGCSHGNHA"
     gene            complement(25709..26605)
                     /gene="lipA"
                     /locus_tag="VFA54_09050"
     CDS             complement(25709..26605)
                     /gene="lipA"
                     /locus_tag="VFA54_09050"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZS50993.1"
                     /translation="MSPLVTIEGLAPRPPWLKAPAPAGENYRELKALVTRLNLHTVCE
                     SAACPNIGECWNARTATFMILGNVCTRRCGFCAVQKGAPLVVDYDEPRRVAEAAKALG
                     LRYAVITSVNRDDRKDGGAELFALVIAAVREQVPGCRIEVLIPDFQGSREALHTVLEA
                     RPDVLNHNIETIPRLYRQVRPGARYERSLDILAGAKRMAPEMPVKSGMMLGLGETGAE
                     ILAVMRDLARCGVDILTLGQYLRPSPRHLPIVRYYEPREFEAFRRAGLELGFAHVEAG
                     PLVRSSYRAAQSELRARQGAKQ"
     gene            26687..28072
                     /locus_tag="VFA54_09055"
     CDS             26687..28072
                     /locus_tag="VFA54_09055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS50994.1"
                     /translation="MDTLTVQFLGAAGTVTGSRHLLSIGKSHLLVDCGLFQGRKSLRL
                     QNWIPFPFPPADLEGIALTHAHLDHTGYLPRLVKEGFRSPVYCSEPTADLLGVLLPDS
                     GHLQEEEAEFANRRGYSKHKPALPLYTAEDAEDALKYVRPVPPGQPVRMENGFQFHFH
                     NSGHILGSKAIHVIINGLRILFTGDLGRFRATGGKPDAIPDSVDYLIMEATYGDRLHP
                     KDDPRPRLAEIIRDTAKKGGAIVIPAFAVERTQKLLFILRGLMDEGLIPQIPIHVDSP
                     MAIEAVNIFLRHSEEYDDGTKAMIKRHGSPLKWPDVHFDKTIEQSKQASASRSPAIII
                     SASGMATGGRVLHHLAQRLPDHRNTVLFVGFQAPDTRGHLIASGAATVRIHGQEVPVR
                     ARVESFEEFSDHADYEEILRWLSSFRSAPRQVLLVHAEPPAAHALEQRIESKFGWPVH
                     VAEQGERVALD"
     gene            28075..29466
                     /gene="lpdA"
                     /locus_tag="VFA54_09060"
     CDS             28075..29466
                     /gene="lpdA"
                     /locus_tag="VFA54_09060"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZS50995.1"
                     /translation="MYDVLIIGSGPGGYVAAIRAGEYGLKVGVIEKDPYLGGVCLHVG
                     CIPTKSLLYSAEIYDHFLHPGEFGISLDNLKLDWSRVQSRKEKTVEKHAKGVEFLFKK
                     YKVETIRGYGRVKAAGQVEVEGPNGKQDLAARSIILATGSEARMLPGLQPDPERILTN
                     VEILKLASIPQSLAIIGAGAVGVEFASIYRRFGSEVTIIEMLPRLVPLEDEEVSAELG
                     RAFRKQGIAMYTGARVERVEKRPDRVALEFRDSGGTTQKLEAEKLLVAVGRKPNTENI
                     GLEKTAIKTDRGFVPVDPWMRTAEPGVYAIGDIVAGSPQLAHVASMEGLVAVGHIAGK
                     PVRPVNYRRIPSCTYCEPQIGSVGLTEAQAKAEGRQVKVGKFGFGANSKSSIIGNHSG
                     FVKVVSDAQYGEILGVHIIGPSATELIAEAVAAMESEATVESMMATIHAHPTLSEAVW
                     DAFNSVYGLTINA"
BASE COUNT         5800 a         8930 c         8999 g         5906 t
ORIGIN      
        1 tctacacaac tacaaggtcc gcgtgacctt gaccggcgcc aatctggatt cgacaggcct
       61 gctttgcgat tttgtggaag tcaagcggat gatggacgcc atcatcgaac gcctcgatca
      121 ccgcttcatc aacgaaattc cacctttcga cgagctgaac ccatcggcgg aaaacctggc
      181 ccgctacttc catcaacagc ttgccctggg gctgcgcgag aagccgcccg ccgtgccggt
      241 gcgcatcggc gaggtgcagg tctgggaaac ggacagcacc acagccacct accgggaata
      301 acgtgcaggt caccgagatt tttcactcga tacaaggcga gtccacgttt accgggctgc
      361 cctgcacgtt cgtgcggctc acggggtgta atctgcggtg cgtctggtgc gacaccgagt
      421 acgcctttta cggcggcgag aaaatgaccg tcgagcaggt tctcgagaaa gtccgcgcct
      481 tcggagggcg tttggtggaa attaccggcg gggagccact cctgcaaaaa gaggtctatc
      541 cgctcatgga gcgcctgttg gacgagggat accaggtgct gctcgagacc agcggggagc
      601 gctccatcgc tcgcgtgccc cgggatgtgg tgaagatcgt ggacgtcaag tgtcccgact
      661 ccggcgaggg cggcgccttt gatctctcga atctcgacgc catcgctccg cacgatcaag
      721 tgaaattcgt catcgcctcc agacgcgatt atgagttcgc ccgcgatttt acccgcgagc
      781 atgatctctg ccgccgggcg gcggccgtga ttttctcgcc tgtgtacggc aaaattgacc
      841 tggaaaggct cgctgcctgg attctcgagg atcgtctgga ggtccgcttc ggctatcaac
      901 tgcacaagct catctggggc gcggacgctc gcggcgtata agcctgtgtc tcgcgtatgt
      961 tgacaggaac cggccgggcc gcgtaccgtt ttcagacgcc gcccgttttg ctggatcgcc
     1021 ggcgtacaaa attcgcgggc ggtgttccac ggaaggaatt tatggccact gaagctgttc
     1081 tcgccgcaac gctggtagaa ccgggaaggt atgaactgcg ggagtatccg ctgcccgagc
     1141 cggcgccggg ttgtgttctg gtgaagatgg agttatccgg aatctgcggg acggataagc
     1201 acacgtacca gggttacacg acccaatact ccggcgcagg cgagggaaag aagattcgct
     1261 ttccgatcat ccagggccac gagaacgtcg ggaccatagc cgccatagga ggcagcggcg
     1321 gctgctccga tttcgaagga gcgccgctcc gggccggcga ccgcgtcgtg gtcggcgcca
     1381 acgtctcctg tggacggtgc tactactgca cgcatgattt tccgtattac ttttgccaga
     1441 acatgacgga ttacggcaac aatctttgcg ccgccgatcc accgcacatt tttggaggct
     1501 ggtcgcaata catttacatc gtgccgggaa gttttttggt ccgcgtaccc gatgaactgc
     1561 ccagcgaaat cgcggtgctc accgaggtga tggccgtgac cgttgggttg gaccgcgcca
     1621 agcaaatgtc ggcgtttccc gatgagccgt tcctgtttga cgacacagtg gcagtgttag
     1681 gagtcggccc tctgggcatg tgcttcctcc tgaaggcccg catgttgggc gccgggacga
     1741 tcatagccat cgacctttcc aactaccggc tcgatttcgc caggcggctc ggagcagacc
     1801 acctcatcaa cgccgccgcc actacgctcg ccgagagact cgagattgtg cgggacttaa
     1861 cgcacgggcg gggcgcggac gtggtcatcg agtgcgccgg cgtaccgcag gcgatccccg
     1921 acgccctcga aatgctgcgg ctgggaggac tgctggtgga agccggaaat ttttcggacc
     1981 tgggcgaggt ccgcatcagc ccgcaccggc acctttgctc gaaaagcgtc cgggttctcg
     2041 gcgtcggcgg agaagagccc gccagctacg ggcccagcat gcggcaaatg gcgcgctaca
     2101 tgcgtttcta cccgctgcaa gaactcgtaa gccaccgctt cccgctgagg gaagtcgagt
     2161 ccgcggttca caaatcgatg gcggccgatt ccatgaaggt cgtcattgag ccatgggcgt
     2221 gagagaatct tccttcaccg cgccgcttgg ccaagcgtcg gcggcgctga cttgaggaga
     2281 acaatacagc atgggaaagc tagccatcac gggaggcgag cctgttcgga aaacccgctt
     2341 gtctgcatgg cccgtgtata cggatgccga ggtcgcggcg ttgcaggatg ttctcgccag
     2401 ccgcaactgg ggagggcagc ctttccccgg caagcatagc gaagcctttg ccaaaaattt
     2461 cgccgaactc cataccgtga agtacgccca gtgcgtcaac accgggacag tggcgatcca
     2521 ggcggcgctg aaggccattg acatccagcc gggcgacgag gtgctggttc cggcttacac
     2581 ctgggaggga accgtcgggc cggttctgct ggtaaacgcc gtgccggtct ttgtagacgt
     2641 tgacccggag acctactgca tcgacccaaa ccagatcgag gaatttattt cgcccagaac
     2701 ccgcgccgta ttgccggttc acctgggtat gcgcttcgcc gatctggacg ccatcatgcg
     2761 gatcgctcaa aagcacggcc tgaaagtcat cgaggactgc gcccacgtgc acggaggcct
     2821 gtggcgcggc aaaggcgccg ggtcgattgg ggatctgggc gctttcagct ttcaatccag
     2881 caagctgatc acttgcggcg agggcggcgc cgtcgttaca aataatctcg agtactttga
     2941 gcgcgtgcag tcttacatca acgcggggcg cgcaagcgtg accgataaat tccgccatcg
     3001 gttgattgga ttcaattacc ggcttggcga atttcaggcg gcggttctcg gcccgcaact
     3061 ggagcgcctt cccgaacagg ctcgaatccg ccaggcgaat atggactact ttgaatcgcg
     3121 actgcggggc gcgccggggc tgggtttctt gagaccggag ccccgaatca cccggcctgc
     3181 tccctacgct tacgtgttga agtatttcgc ggagcaggca aaacagatcc cccgcgccgc
     3241 tttcgtcgcc gcgctgcaac tggagggcat tccgtgcgac ggcctgtttt acgagccggt
     3301 ctaccggagt tcgctcttcc cggtcaatcc ctgcgaatat ccggcattaa gctggggtcg
     3361 gcccgcgcct cttgagctta gaaatatgta ccggtgcccg gcttccgaaa gagccgctta
     3421 tcaggaggcg gtctggtttc cccatcatct attcttggga agccaggccg atacggatac
     3481 gattgtggcc gcggtccaca aggttctcga aaacatcgag gaactgcgcg gcgtggagca
     3541 tcccgccata gaggcgcaaa acctgagccg tgctgatcgg gaaagctgat gaggaacgcc
     3601 gaagccgtct tcccggcccg gatcacaggt ccccgtcttt gctcaccggg ggcaacgtga
     3661 agccggtcgg atatgccgtg gtcggtctgg gcgcgattgc gcagcaagcg gtgttgcccg
     3721 ctttcgcgca cagcaacaag gccaggctgg ccgcagtggt cagcggcgac aaggaaaagg
     3781 cggaacagtt ggccggaaaa tttcgggcgc ctcactatta cacctatgaa gcctacgccg
     3841 agtgtctgaa gaacccggcg gtggaagccg tttacattgc caccccgccg ggcaggcacg
     3901 aaaccttcac tttggatgcc gcccgtgctg gcaagcatgt actctgcgag aagcctctgg
     3961 cggcaaccgt ggccgagggc aggaggatgg tgcacgcctg ccgcaaaaac ggcgtccgcc
     4021 tgatgactgc ttacagaaag tacttcgaac cggccagcgc agcgctgaaa aagatgattg
     4081 ccgaaggcag gctgggcagg gtcgatatta ttcatacagc atttaccgaa tttcaccgag
     4141 cgggcggcag cgcccctccg tggctgctgc aacgaaagct ggcgggagga ggcccgttga
     4201 tggacctggg cgtctactgt gtgaatacgg gccgctggct cgtggatgag gaccctgtcc
     4261 aggcggctgc cttctcctgg cgtcacgacc gaaaacggtt ccgccaagtc gagcagggca
     4321 ttgcatttcg cctgaatttt gcgagcggcc tggtgctgca aggcagctcc agctacgggt
     4381 cagctctggc ctcgttcgta cagatccacg gtgagaaggg ctgggcggct ctttctcccg
     4441 cgtttgcatt tgagcaggag cggagattgg cgggcagtat cgccggaaga tggttcgaga
     4501 tgcgatttgg agtgattgac gaatttgcgc tggagctgga cgcttttgcc gaatgcatcc
     4561 gcagaaatcg gccggcggaa ccggatggag agcagggcat gcgggacctc atcatcatcg
     4621 acgctattta ccggtcggcg aggcagggcc gtcctgtccg catccgctac tcttaggctc
     4681 cttcatcgca gccggcgccc tttttctgat atgcgaggag cgtgagggga gatcgaatgg
     4741 ccaagctggg cctgggcgtg gtgggagtcg gcacaatggg ccgccggcat gcggaaaata
     4801 tccgccgcct ggttcccgaa gcccggctga ttgccgtggc cgatgtggat ctggcgaggg
     4861 cccgccaggt tgccggggaa ctcgagattg agcattatta cggcagtgtg gaagcgctcg
     4921 tcgagcgcaa ggatattcaa gcggttgcgg tggcgactcc cgccaagttt cacggcgcag
     4981 caatccggtc gtgcgcggaa gccggcaaag atgttttctg cgagaagcct ctaaccctga
     5041 ccgtgcagga ggccgacgag attctcgccc tcgcggcgaa agcaggcatc cgcgtgcagc
     5101 cggggcatgt ccgtcgctat gaccccccca acgtcaaggc caagaagcgc atcgaggccg
     5161 gcgaaatcgg agatcctgtg attttcaaat cgctggcgcg cgatccggcg ccccctccgg
     5221 tcagttatat gcagagcggc tggaatggca tgtttttcca cgattccacc gtacacgagt
     5281 tcgacctcgg ccgatggctg atggacgacg aaattgcaga gctgcacgcc tacggagccg
     5341 tgctggtgtt ccccgaaatc gcgcaattca acgacatcga cacggccctg gtgaacatga
     5401 aattttcgcg cggcgccctg ggaagcgtcg aaaactatat gcaggcctgc tacggatacg
     5461 atgtgcgaac cgaaatcgtc ggctcgaaag gcgccatcct gatgggatcg ttgcagcaaa
     5521 cctcggaagt ggttctcacg gcgtcgggcg caaagacgga tatcgtggat cactttctgg
     5581 tgcagtttgc ggatgcctat cttgaagaaa tgcgggattt cgtccgcacg attctcgcgg
     5641 atctgaagcc taaagtgact gcgttcgacg gacgccaggc ggtcgcggcc gccgcggcgg
     5701 ccgagcgctc ctatcgcgaa ggccttccgg tgaaattgga tcgccagcca gcatgaattc
     5761 cacaccgggc tgcgcagacc tgagcgctcg ctcctgttgc ccaagcctgg gaggcttctc
     5821 gcgcccgccg ctggccttgt gctggcggac gccgatcaat ccatccgcag ctttgtctgt
     5881 gagcaagcct tcagcttgcc cggtgagggg ccgccggccg agcctgggag ccctcggccg
     5941 cttagaaata ccgctgcaaa tcggcgaagc gttccacgat cagcaatcgg ccggctcttc
     6001 tttcccccgg ggagacgatc tcttgttttt cgagcgacca ggtgtggggg tggggtacca
     6061 gcacggcgtt caatcccgct tccagcgccg gattgatgtc cgatttcggg ctgttgccta
     6121 ccatccaggt ggcgccagga tccaagtttt tcgtctccac tacgcgccgg tagttatgag
     6181 cgtctttctc cttcacgact tcgcagtcct cgaaatagcg gccgagtccg gagcggccta
     6241 gctttaactt ttgttcctct gggtgacctt tggtgaacag cagcagacgc tttcgcccag
     6301 ccagataacg aagcgtgtcc tccacgccct cgatcacctc catcgggcgc tccaaaatcg
     6361 cgcccgcaaa acccaggacc atcgccaggt cgccatcgcc atacggccgt tccgccagac
     6421 ggcggaagca ctcctggaga ttccgcccaa aattggcgga cccatacccg tgcagcttga
     6481 tgttcgcggc ttcgatctcg tccagcacct cgcggatctc ggcggcagaa agccgggaat
     6541 gatcgaggaa cgccacgaat tgctcaaaag cctgctcgaa atagatgttg ttttcccaaa
     6601 gggtgtcgtc ggcgtcaatt aaaagccaat cgcgcatgtt ggcaatccgg cagccgccac
     6661 caccaatgta gcccaactcg caggcaaaag ctcccgcgct gcctggcccg ccgtcccttg
     6721 ccggacctcg ggcaggcctc tccgagcggc atccaggcgt aggctggtac aatgacaagc
     6781 ggctctctat gcacgttaaa gtgctatttt tcgggatgtt gaaagacttg gtgggccgcg
     6841 ccgaagaaca gatcgaggtg cccgaagggg cccggctttc aaacatcttt gagcaatact
     6901 gccggcgctt tccccggctg gcagagatgg ccggcagcat cgtgctggcc cgcaaccagg
     6961 agttcgcggg acccgaagcc aggattggcg agggcgatga aatcgcgctg ctgcccccgg
     7021 tgagcggcgg cagctcgccg gcgccggaag ccgcatccac ggctaatagg accgcggggc
     7081 cggactctgg gattctcgcc gagtttgagg atgaacttgg caactacttc gcgattaccc
     7141 gcgtcccaat tgatgcccgg gcgctggcgg cgcgccttgt ccggggatgc gacggttcgg
     7201 tgattacatt tgaaggaatc gtccgcgaca actccaaagg gcgcgcgacc ctgtatctcg
     7261 aatacgacgg ttacgttcct ctggcgataa agaaaatgcg cgagatcgct tccgacctgc
     7321 tggctgcgca cggggcggac cgcattgcga tggtgcaccg gctgggccgg atggagatcg
     7381 gggaaaccag cgtcgtcatc gtagtgactt ccgcccatcg caagcccgct tatgaggtga
     7441 gccgcgaggc catcgaccgc ctgaaacgtc tggtgccaat ctggaagaaa gagcacttcg
     7501 ccgacggcga ggtttgggtc gaaggcgaat gggatgcttc cgttcctcgc ctggagggaa
     7561 gctcgcggtg ttccggttaa gcatattcgc cgcgtgtctg ctggcctgcc tgtgcgccca
     7621 ggagcaaccg ggcagattca acgtagaagt caagctcgtc actgtgctgg caacggtaaa
     7681 gaacaaccag ggcgtcccca ccggggacct tgagcgcgag gacttcacac ttctcgcggc
     7741 aggcgtccct caaaaaatcg ccgtatttga gcgccagacc gatcgtcctc tttcggtcgc
     7801 gcttttgttc gacgcgagcc cgagcgtggc caaggaactg cgcttcgagc aggaggcggc
     7861 ggttcgcttt atccgcagcc tgctcggctc cggagcgaac ccggctgacc gcatcgccgt
     7921 gtataagttt tccgattttg tggatgaggt gcagggcttc accaactccg tgcagcggct
     7981 ggagcgcgcc gccctttcca tccgcaccca tggcggaaca tcgctgtacg acgcgctcta
     8041 tctggcggcg cagggactcc agaaccggga aggccgcaag gtgattgtgg tcattaccga
     8101 cggcgatgac acgacgagcg acatcaagtt ctcgcaggcg ctggaggccg ttcagcttgc
     8161 cgacgccgtc gtatattcga tcattgtcgt gccgatcacg aacgatgctg gacggaacct
     8221 gggcggggag aatgcgctga aaaccatgtc cgccgccacc ggaggtctgg cgtttttgca
     8281 acacgccggt caggatttgg acagcaattt ccggcagatt gagcgcgact tgcgcttgca
     8341 gtacctgatc gggttttacc cgcagggcgt gcccgccggg ccggaccgct ttcaccgcat
     8401 cgagttgaag gtgaaacggc ccggcatgaa agcgctggcc cgcacgggat atttctcggg
     8461 tccggaggag gagccggtca cctcgtatgc ccccgcggtc gttgtggatg gcagccctcc
     8521 ccgagaacga aagaaagaag cgccggcggc ccgcggcgga aagagcagtc ctaggagcat
     8581 accttgaaag gtccattcaa gcctcgattc aatcggccgc cccacggcgg cggccagggc
     8641 ggcggcggaa gcaaatccca taaggccccc gaccaaacca tgcaggaggt gcaatatctg
     8701 aaggagttga tcgaaaaaca ggctaccgtg tgcatcaaac tgcgcaataa cgaggaagtg
     8761 cggggtacga tcgagtacta tgacaatgct ttcattcgcg tcactcgcgc ttccgccccg
     8821 aatcttttca ttttcaagca cgaaatcaaa tacctgtacg aggaagcaaa atagccgccc
     8881 aggcagcggc ctgtgcttca tccaggcacg ggcggcgccg caaggctggc aacgggaact
     8941 ctgctatgtc ctcatcctac ctttctgccg catcggagat cgcccgcgaa gcaggggctc
     9001 tgctggcccg ctattttgag cggcgtgtgg cagtcgaata caagggcgaa ttcgatgtgg
     9061 tcaccgaggc ggatcgggct tcggaaagcc tgattgtgag ccggctgagg acccggttcc
     9121 cgcaccattc gatcgtcgcg gaagaagggt ctggcataga gcgctcttcc gagttcacct
     9181 ggtatgtcga tccgctcgac ggaactacga acttcgccca tggctttccc atgttcgccg
     9241 ttacgatggg actcgagcag gcgggggaat tggtggcggc tgtgacgtac gaccccctgc
     9301 gcgatgagtt cttctcggcg gaaaagggcg gcggagcgta tctgaacagc cggcgtttgc
     9361 aggtctcccg cgttagccgg ctttcagaaa gcctgctggc cactggtttc cccagcagga
     9421 aacggcatgg cgacgtgaat atctacttct accaccaggc cgctatgctg acacacggcg
     9481 tccgccgggc cgggtcagca gctctcgacc tggcgtatgt ggcggccgga cgtttggatg
     9541 gtttctggga gttccggctt aatgcttggg atattgcggc ggggcttctg ctggtgcgtg
     9601 aggccggggg ctgctattcg gatatgcacg gggccgagca tcgtttcgat agcccggaac
     9661 tgctggcctc caacggtctg attcaccgcg aactgctgtc tctttttgga gacattttcg
     9721 ccaatcgtat ccgggtgccc ttgccggcgc tcgccggcgc cgtccctcca agctaaatgc
     9781 tgattcccta agctcctgct ttacaatgtt ttgcctggaa tggtagcctg ttgaattcgt
     9841 aggtagaatc gaccgttttt cgcctgctgc tgaaagggga aaggatgtgc tgtattcttt
     9901 aaaatgcgaa ctgtcgattt gatccggaaa aaacgggacg gggaagagct tacagaaaaa
     9961 gaaatcgact acctgattca aaattacacg cggggcgaga tccccgatta tcagatggcc
    10021 gcctttctca tggcggtgta ttactcgaac atgagcgaaa gcgaggtgac cgcgctcacc
    10081 ttggccatgc tcaattcggg ggagaccctg gatctcagcg acctgccggg cgccaagatc
    10141 agcaaacatt ccacgggcgg ggtaggcgac aagaccagtc tcattgttgg ccctatcgcg
    10201 gcggcggccg gcgtggcggt gccgcagata acgggccgcg ggctgggcca caccggtggg
    10261 acgcttgaca aactggaatc gatccccggc ttccgaacca atctctcgat cgaagaattc
    10321 aagactgtgc tgaaggagca caagctttcg tttatcggcc agaccgaccg gattgctccg
    10381 gcagacaaga aaatgtacgc gctgcgggac gccacggcga gcgtcgaatc cttgcctctg
    10441 attgcggcct ccatcatgtc gaaaaaactg gccgagggaa tcgacggctt gatcctggac
    10501 gtcaaggtcg gctccggcgc tttcatgaag aagcagattg aagcccgtaa gctggcgcag
    10561 ttgatggtga cgatcggccg ccgcatgggc aaacgggtgc aggcgctgct gaccgatatg
    10621 gatcagccgc tcggctacgc cgtaggcaat gcgctggagg taatggaggt ttcacagacg
    10681 ctgcgaaacg agggtccggc tgatctcaca cagctttcgg tggaactggc ggctcggatg
    10741 atccacctgg cgaatccctc gcagccgatc gacagcgcgc gggagcgggc cggagaactg
    10801 ctggcttcgg gagaagcgct ggccaagctc cagcaggtga tcgcggccca gggcggcaac
    10861 cccgaagcgt tgaaccaatt tgagctgctg ccgaacgcca cgggcgcgag agagatcatt
    10921 tctccccggg ccggctacgt aactcgcatt aacgccgagg acgtggggca ggcttcggta
    10981 ctgcttgggg ccggccgcga cacaatggag aagccgatcg acccggcggt gggcatcatt
    11041 ctggaacgaa aagtcggtga gaaggtgccc gccgggggcc ggctgtgtac tctctattac
    11101 accgacgaga cgcatttgga agaggccgcc caacttgttg aggacgcctt tcacatctcg
    11161 gccaacgccg ccgagcctcg cgaactcgtg ctggaagtgg ttcagtagcc cggaagccgg
    11221 ggatcgcgat ctcagaagcc gctccgcgaa cactgcctgg gccccgcgct ctgttctcct
    11281 gtgcgtgttc tcactttttt gagtatgtcc cgttttaccg gtctgcttgg cctcgcggcg
    11341 attatcgggg gcgcgtatgc gttttcatcc aggcgcaaga gcattcctgt cagagtagtc
    11401 gcttggggcc tgggcttgca atttgccctg gcgctgttgg tgctgcgcac gccctttggc
    11461 ctcctgttcc agtggatcgg agccggcgta aatcacatgc tcgatttcgc ccaagccggc
    11521 agcgagttcg ttttcggacc tctggcgaaa gctaatgggc cggcgggagt cgtgttcgct
    11581 tttcacgtcc tccccgtcat tatctttgtc gcttcttttt tcgccgtgct ctattacctc
    11641 ggcgtcatgc aggctatcgt gaaaggaatt gcaatagcaa tgcaacgggt catgggctcg
    11701 agcggggcgg aatccctgga tgtggccgcc agcattttta tgggccagac ggaggctccc
    11761 ctcaccatcc ggccttttct gccggggctc accgagtcgg agctgctcac ggtgatggcc
    11821 gccggaatgg cgcacgtctc cggagccttg atcgtcgcct acgtggatat cgcccatgtc
    11881 gaggccaagc atctgcttac agcggtcatc atgactgcgc ctgcaacaat catgctttcc
    11941 aaaatgctca ttccggaaac cgagcagccg gagacggccg gaggcgtcga actgcatgtc
    12001 gagaagctcg atgtaaacgt gatcgacgcg gccgcgcgag gcgctaccga ggggttgcat
    12061 cttgcgttga acgtggcggc gatgctcgtc gccttcatcg ccctaatcgc tctcggcaac
    12121 ggattgctcg gcgggttaca ctcgattcgc ggttttggct gggctcccga ttcgctccag
    12181 cggctctttg gctgggtttt cgcgccggtt gcctggatta tgggggtgag ctggcgcgat
    12241 gcgcccgccg tcggcaacct tctcggcaca cgcatggtgc tgaacgagtt tgtttcttat
    12301 gtccttctgg gacccatgaa gcctcagctc gagcccaaat ccttcgtgat cgcaacgttc
    12361 gctctttgcg gttttgcaaa cttttcctcg atcgccatcc aggtgggcgg gatcggcgct
    12421 ttggccccga cgcgccgcca cgacctggcg cggctcgggc tgcgcgcgat gatcaccggc
    12481 acgctggcca acttcatgtc ggcggccatt gcgggaatct tgctgtgaaa cgctcgcttg
    12541 ctctgctgcg ccgccgatcg ggaatcgtgc gagccatatt ttgagggtag atcgaagtgc
    12601 gatccttgct tcttccacga gcccatcccg caaaggccag gaagggatct ctcaactact
    12661 tgcaagtgga gaaagcctgc cgctttttga tctcgcatct cgaggttgtt ccggcagccg
    12721 ccgtggtgct gggcagtggg ctcgaggcat ttgccggcga gctttcgaac ccggtcgaga
    12781 tttcctatgg caagatccct cattggccgc gcgctaccgt cgcggggcac gccggccggc
    12841 ttgtcggcgg agtgataggc ggcaagcccg tcgctgtgtt ggccggccga gcgcatctct
    12901 acgaaggcta ttccgcgctg gagattacat ttcccattcg cgttctgggc ctggctggga
    12961 tcaagacgct ggtgcttacc aacgcggccg gcggcatcaa tccccaattt catccgccgc
    13021 agcttgttct tataaccgac cacatcaatc tgcaaggcgc aaatccgctc attggggtga
    13081 gtgatgagcg cttcgggccg cgcttccccg atctcaccga agtttacccc aagcgcctgc
    13141 gggatctggc ccggcaatgc gcgcgggaaa tcggccgcgc ccacggcctc gagcttcccg
    13201 aaggcgtcta cgccgcggtc tccgggccaa gctatgaaac cccggcggag atccggtttc
    13261 tacggacgat tggcgcggac atggtgggca tgtccacggt tcccgaggct atcgtcgccg
    13321 cccagatggg cattccggtg ctcggaattt ccatgatttc caacatggct gcgggcatgc
    13381 tgccgcaaaa gatcagtcac caggaagtgc tcgaagcggg ccggcgcatg agtggcacgc
    13441 tgattacgct cttaaagaag ctgattccac gactgtgaac ccgcaagaac tgatccagag
    13501 cgccgtcaca gcccgcgagc acgcccgcgc cccgttttcc cattttcggg taggcgcggc
    13561 cattgaaacc cgcagcggcg ctgtttacac cggctgcaat attgaaaacg ccacctatgg
    13621 tctcaccgtt tgcgccgagc gagtcgccgt atggaaagcc ctttctgagg gcgaagcgga
    13681 gtttatccgt atcgccatcg cggcggatac ggagactctc acgccgccct gcggagcttg
    13741 ccgccagatt ctgtgggaat actgcggcga catcgaaatt attctggcca atctcggcgg
    13801 caagacggaa accatgcctc ttgccgcttt gcttcccagg ccgttcgatg ctgcatttct
    13861 ggaagaatag cgcggtctta gaagacgttc ggcggaatcg ggaaagaggg gggcattccc
    13921 ccccagccgc gccggatcgc cggctcggaa ttcttccgag cgccttcaac ccggtcaccg
    13981 ttgcccatgt ggaactggcg cagcaagcaa tgctccagta ccggctggat gaggttctct
    14041 tgactctgcc tcgcgccttt cctcacaaag gttacgtggg gccaaccttt gaagagcgca
    14101 tcgagatgct gctggccgcc gccgccgagg agccccgctt atcggccggc tcaaccgatg
    14161 gcggtctctt catcgagatt gtccgcgaat gccgtgaagt ttatggctgc gccgcggagt
    14221 tctttatcgt ttgcggcagt gatgcagccg agcgggcgat cgcctgggac tacgcgcggc
    14281 aggcgcctgc ggccggtcct ttcgccgagc aaatcaagga gtttcaaatg ctcgtggcgc
    14341 cgcgcggcgg gccgtggacc gtgccgcccg agtacgccgg ccggatccac ctcattgcgt
    14401 ggccgcccga gctcgaccta tgctcctcat cggccgttcg cgccgccatc gcccaaggcc
    14461 gcccctggga gcacctggtc cccccgcccg tcgccgcgat tatccgccgg cgtagcttgt
    14521 attccaatac ctaggcggag cctaaggtgt ttcgttcccc ggattccaga tctctagtta
    14581 atagctggcc cgacttcttg cggcgtatag acgtatgggg agagaaacgg gatatgacga
    14641 ccaccgccgc cgaggtttac tgtgacgcct gccatctggc aatcgggctc cagaagcacg
    14701 ctatttgcaa aaagcgaaga aagggaaatc cgatcgaata tgtttactac cacaatcgcg
    14761 acgccgatga ttgctggggc aaacagttga aggctctaat gcgaaaatac aacggtaagg
    14821 ctcgctaata ggagttaccg taaaagaagt cacgcgacac ggtggaacgg ccgccgcaat
    14881 cccgccggac tgcggagatc cggctgcgcg cctatcccac cttctccacc cgcacggccg
    14941 cgttatagtc cggctcgcgc gaagacggat cgatgacctc acgggagagc aggcagttgc
    15001 cttcgggcca atgcacttcg aggttgccgg gctttacctt gtcaattttg gcacggccca
    15061 catagcttcc gctggccgaa ctgaggcgaa tcagatcacc ctcccgaatc cccaagctcc
    15121 gggcgtcttc gcccgaaatc aggatgctgt cgcgcgaggc gccggtgagc ggatcggccg
    15181 cccgctgcac catggaatta aattgcttgc cgcgcctggt ggaaacgtaa aacatccctt
    15241 ccggacgccg gtgctcttgc aaggacacaa ccgaaaaatg ggctttgccg tcgggtgtgg
    15301 cgaattggcc gttggcgaag agacgttcgc cgccccactg gaagttgtcg ccctctttcg
    15361 ccagcctctc gatgccgctg tacaaaggaa ccgctctgct gatctcctcc cggatcgccg
    15421 caacggagtg aaaccgcacc cggtcggcca gattcggtcg gacgcgagcg gcgacctcgc
    15481 cgaacacctc ccattccggt ttggcggaac cgatgcggcg gcccggaatt tcgggggaga
    15541 agatgatccg ccgctcagtg gtcgtttccg tccctccgcc aggcgattcg tagcgtgtcg
    15601 tcgcgggaaa cagaatcacg gtgtccttgg gcggcagcaa catcatggaa gaaaggacaa
    15661 cgtcctggtg aatgcgtgtc ggaatatttt cgagagcctg cgcggtcgtt gacgggcccg
    15721 gaagcgtctc taagaagttc ccgccggtcg tccagaaggc atcgatccgc ccttggcagg
    15781 ccgcgttcac catctcggcc gcggtaagtc ccggaaacgc gggtaagaca aacccccaca
    15841 cctgttcgaa ccgtgcccgt tgttgatcat cgagtcctgg aacacacccc acctcggcgc
    15901 ccccttgcac ccccgagtgg ccccgtatcg gcattaagcc tgtatgctcg cggccgaccc
    15961 atcctcgagc aagcgccacg ttcaccagca tttcaatggt ctggacgccg tgcgcgtgct
    16021 gggtcaggcc catggaccag acgaagattc cattgcgcgc ctgatgcagc atgcctgcga
    16081 atcgccgcat ctgctcccgc gtggttccgc tttcccgctc aagcagttcc caatcttgtt
    16141 gctcggcgca ggtctttgcc gcttcgaatc ccgtggtatg ccgggcgatg aaatcagtgt
    16201 cgatccaacc ttcggtgttc agaatcctga agacgccggt tagaaaagcc aaatcgccgc
    16261 cggtatcaac agcgaaccaa tcgtctgcca ttttcgttcc aaaaacggcg gactcgaaaa
    16321 tggaaggaat ccaatagctt tgaagacccg gctcaaagta cgggttcaca atggctaccc
    16381 ttgttcccgc ctggcgcgcg taatacagat acttggtcgt aaccggctgg ttattgggcg
    16441 tattggagcc gaaaaagaca atcaagtctg cgccgatcca atcgcggtag gagcaggtgg
    16501 tcgcgccgta acccagggtt ttcttcattg ccgtggtgga agcggcatgg cagaggcgcg
    16561 ccgagttatc tacgtgattt gtgccgatcg cccgcgcggc tttttgggca gcgtagtagt
    16621 gctcgttcaa gattccgcgc gaggtgagat agacagcgaa ccgctcgggc cgcgtgccgc
    16681 gaattttctc ggcggctatg tcataggctt cctcccagga gatggctcga aaacctttct
    16741 ctttccgccg ccggatcatc ggttccggca gccggcccag cccgcgcaat tgctgcgagt
    16801 tgagatgctc gattgagctt acgtcggcca gccggccggg gtcgagagcc ggcgcggtgt
    16861 tcaggcgcat caattcgagc cgcaccatac agaggtggat cccctccatg gtccagtcct
    16921 tcatacctgc tgtgcccagg gcgcatccat cacaaactcc gtggttcaga atccgccagg
    16981 cataagggag ctggtcgcgg ttttcccacg cggcgcggag catttcggcg taatgattcg
    17041 gcttcctccg cgcgggatgc cgcgggatct ccgacgccca tagattgcgg ttccagttca
    17101 tttccactct gatcgggcgc tctcggcccg tccaccgccc ttagtgtagc gccgcttgcg
    17161 aacgcggcgg gtggccggag gatgcagggc gtcaggggtc gggcgccggc ttgcggaatg
    17221 aatgcatagc tattagtgcg cgggaaggcc cctgccctgg tgggcgggtt ttctgagcag
    17281 cagcagcaga ggtatgacga gcagaaaaat caatcccagc agccggaacg tattcagata
    17341 cgcgagcacg gcggcctgct gctgcaccaa tccgaataag gcgccttgcg ccttatgtgc
    17401 ggcagtgacc gggtcgaagc cgcgcgatat gaaggcgagt tgcagcgctt ggaatacatt
    17461 gtgcgcttgc aaagaaccgg gagtgacgtt cgcgccgaaa acgttcgtga acctttgcgt
    17521 agaccgcgtg ttgagcgtgg tcgccatcgc gatccccacg ctgccgccta cgttgcgcat
    17581 gaggttgaac aagcttgtgg cgttacccat ctgctctttg ggaatggggt ccatggtcac
    17641 cgtggtgaga ggaacgaaca agagcccgag agaaagaccc tggagaaact gcggccagaa
    17701 gaagtcccaa aatccggcgt tcaggttaag ccaggaaagc tgaatgagcg tgaaaccggc
    17761 gcccaagaag cccagcgcca gcagcttgcg tggatcgaac ttcgccacca ggattccgat
    17821 caccggcatc gcgataaacg atcccagccc gcggggaaac atcgtcacgc ccgccttcag
    17881 ggccggatag ccgagcagtt cctgcaacag caaaggcagt acgaccaggc tcccatacag
    17941 cccaaatccc aaaatcgtca tcaacaggac gccggtgccg taggtacgct ccttcaagac
    18001 cctcaagttc accacagggc tgcgggcgcg cagctcgtaa atgataaaca caatcagcac
    18061 gctggcggag aggaccacca gctcggtgat ccacgcggtc gaaaaccaat ccttctcctc
    18121 gcccttgtcc agcacgatct gaagcgcccc gattccgacc gccagcatcc cgatgcccca
    18181 ataatcaatg cctgttttgg gccggcggat ataaggaggg tcgaagatga acatctttgt
    18241 catcaggatg gaggcgacgc cgacggggat gttgatgtag aacacccagc gccagctata
    18301 gctgtccgtc aaccatccgc ccagcacggg gcccagcatg ggagcgacga cgatgcccaa
    18361 tccccaaaag cccatcgcct tgccgcggtc ctccggcggg aaggcttcga gtagaacagc
    18421 ttgcgagatc ggctgcaaag tgcctcccgt ggcgccctgc acgacgcgaa agaaaatcag
    18481 cgtcgccaga ttgggcgcca ggccgcacat gaaggatgcc gttgtgaacc cggcgaccgc
    18541 ggccatcagc agccgcttcc ggccgaagta attcgccagc cagccggtga tcggaagaat
    18601 aatggcgttg gccaccagat acgacgtgag cacccaggtg gcttcatcgg tgctggcgga
    18661 aagattgcca gcaatgtggg ggagcgacac gttgacgacg gtagtgtcca acacctccat
    18721 gaaggtggac agcatcaccg aaattgcgat gatccaggga ttgacgtcgg gagctgccac
    18781 ggtagcagga gcgccgactg cttcagttgc catagtgaag gttcacgctt tgagacccgc
    18841 cgcgcacaga attgtttcca ccactctttc cagcccgatg ctcgagcaga ccgccagatc
    18901 gtagagatga cgattcgtcc aatcctggcc gaaataacgg cggatgtaag cggcccgccg
    18961 gcggtcgctc tctatggccg ccgcctcggg atcagcttcc ggcgggagct gttcgcggag
    19021 cctgcggacg cgctcttcca agggggcgta gatcgagaca tgaaaagcat cctcccgatt
    19081 ctggagaatg cattgcccgc cgcggccgac gatcacgcaa tcgcccatag ctccggtctc
    19141 ttcgatgacc cggctccaca gccgggccat agtttcggcg tcgaacaatt caccctccac
    19201 cctgctggcc gcgccaacga agccgccctg ccaaagcgcc ttcataagcc ggtgaaacca
    19261 ggggtcgacg cactcatcgt agcgccgcgc aatagcgggg ttaacctgag cctttttcgc
    19321 gatttccgtt atcagggcgt cgtccaccag cttccatttg aggcgctcgg ccaacatgcg
    19381 cgcgacagcg ccgcctccgc ttccgtactc tctgctgatt gttatgacac ggatcatccg
    19441 cttggattca tttgacgatc acggtgacct cgaccgacat gccgggacgc aggaggtggt
    19501 cggggtcctg tcctttgtcc agctcgattc tcaccgggat gcgctgcacc acttttacat
    19561 aatttccggt ggcattttcc ggcgggagca agctgaattt ctcacccgtg gcgggggaga
    19621 cgctctgcac ccgcccggag tatttccggc ctccataggt gtctacggaa acgattgccg
    19681 gctggcccgg ccgcatggaa tgaagctggg tctccttgaa attcgccgtg acataaatat
    19741 cttcgagcgg cacaacggcc atcagcgctt gtccgggctg gaccacctgc cccacctgga
    19801 ccgtcttatt ccccacggcc ccggcaatcg gggcgcggat gattgtgtat tgcaaattca
    19861 actcagccag gttcacggcc gccctggctt gcccgatcct cgcttccgcg ttccgatact
    19921 gcgcctggct ggaggcgact tgctgcgggg ccgtttgggc cgcccgcacg ccggcttttg
    19981 cctccgcgac agtgccgcga gcttgggcca cctggctttc tgccacgagg attccttgct
    20041 cggcacggcg aacgtcggcc cgggcggatt ccacagccgc tccggtggca gcttcggccg
    20101 ccacggcggc atcgaactgc tggcggggaa tttcgtcttt ggctaccagt tccttgtaac
    20161 gaaccagatc ctgcgccgcc ttcgcatgat tggcctcggc ttcgcgcagc ttcgcctgca
    20221 taacgtttaa ttgggcgttc gccgcagcca cctgtttctc cgccgcgctc agagccgcat
    20281 tggcgctcag caagcgagct tccgcgctgc tcagcgtgct ctccgtggtg gtggcggtaa
    20341 tcggcacgcc ggtttgggcc gcgcgcgcag ccgcttcggc agcggccaaa tccgcgcgcg
    20401 cccgctccag agcgacctga taatcgctcg gatcgatttg cgccagcacc gcgccggctt
    20461 cgacgtgttg attatccgcc acattgacgg cgatgatcgg acctcctact cgcgcgctga
    20521 tcggcgccat gtgcgcgtcc acctgagcgt cgtcagtcgt ttcacggctc gagtagtatc
    20581 gccatgtcca gtaggccccg gccgccacaa gaattatggc ggcaagcagg gcccatttcg
    20641 ccagcggcct ccggcggaaa aaggatgccc gccggggggt ccaggaccgc tccggctgac
    20701 cgagctgggt ttgtgtttgc gcctgcgggc cgccggaagc ttgctggcgc tgttctgttt
    20761 gtattgcgtc agccactatg gcgttccctc caaaaactga atgatggttt tctcggcggc
    20821 tccaatggcg cgagccagcg aagccttcgc gagattgtac gagtaaagac tggagatgta
    20881 gttttcattg gctgtagcta cggcttcttg cgcctgcacc acttcgatat tgtcggcgac
    20941 gccggccgca aaacgatccc gcgactgggc cagttgctga ttcgccagtt tcaaggcgct
    21001 ttcggcgacc ctgacctgct cgctgttggc cctcaagtcc aaaaacgccg cgcggatttc
    21061 gtaatcaata cggccccgca ggtcctcgct ttccgatctc ctttgccgca agagcgcttc
    21121 cgcctggagg acctcgcctc gaatgcggcc gcctcgaaaa accggcacgc tcagagtccc
    21181 cgagaccgaa aatgttccgt gagagtcagt caggcccggg ccaatggtcc catagtttgc
    21241 gtcggcggac agagtggggt agtgctgggc ggcggccgcc ttgcgcgcct cttccgccga
    21301 gcggagcaaa gcaagcgcat tctggtaatc ggcccgggac cggtacgcct ggtccagcgc
    21361 ctgctcgtaa ctgaccgaag gcggcggcgt gtaagggatg cgcgtcgaaa gctcgaattg
    21421 ctgcccgagg ggcaaaccga tcgcccgggc cagattgagt ttctgcttct cgtactcgtt
    21481 ttggaagaaa atcaaccgct gctgttgggc ttgcagctcg acctgggcgc gcgtcacctc
    21541 gatgcctgcg accactccag ctcctctttt atccacggcc tggtcgtaca aggcttgggc
    21601 cgtctttact tgcgcttcgg ccgcgtcgat acgagccagg ccggcagaag cctgaagata
    21661 aaggccggcc acgccgagcg ccacgatgtc acgcgcatcg ctgtaagagt accgcgccgc
    21721 tctcaagttc tgcgtcgccg agcgcagatt gtgaagggcg ttgaaattca gcacatcctg
    21781 ggaaacatag gcgcgcgcgt cgagcacggt aaaggggccc actatgctgg gcacgccggg
    21841 aaagccgccc ggaaacccga gcgccttcaa attgatttgc tgatcagttc ccgctatatc
    21901 ggcacggagg ttgggcagca aagcgctcct tgcccggtac ttctcgccca gcgcaaactg
    21961 tgtgttttgt tggtttagta gtacgcccag gttgaatcgc agcccgcgat tcacggcttc
    22021 ctccagagaa agggcaagca ctcctggaac gggtttttcc atcgacgcgc tgcccaaaaa
    22081 cggattcggc ggctgctggc taaaagtaac tcccggcgtt tgcggcacgg gcgcggctgt
    22141 ctggggcggc agttgcggaa cttgggacaa cgcctggccg gccgccgcca gccacatgca
    22201 tacaggtccc agtgcgatca aggcggcttt cattggtaga aagattcttc tgagcgtgag
    22261 ttgttcccct taaagccaaa atgtttcctg gaaaaaggcc gcgcgatgca accgcaggcc
    22321 ctaattcacg acgctgacct aatttgcgcc attcataacg gcatcgtttt gattctctca
    22381 caccgttgga tgaacctgtt gccgtgaaag ttgcttcccg gacaaaaatc gcgcttcccg
    22441 tcgctttgcc cgtccgccgt ggagctaagg ttttggaaga tgcggcgtgc attatttccc
    22501 ttaccgttta gctgcccgaa agatcggatt catttggtac caagggtaga ttgcgcgggg
    22561 aaatccgccg cgtttattgt agccatgcaa gcggcgagtg gacggcaacg tgcgccgcac
    22621 ggtccaagga gcatgttttg atgcgtttac gttcagtttc ccgcagcaga atcagaccaa
    22681 gaggtttcgt actgatcacc gtagcaatta cgatcgtcgc tttgttgggc atcatgggaa
    22741 tgtgtatcga cttgtcccga atgtatattg ttcgaaacga gttgcaagcc tatgcggacg
    22801 cggcttccct ggccgcggcc cggcatctgg atggaaccag cctcggcatc agcaacgcga
    22861 cggcgatcgc caaaaattat cccgattctt ataacttttc tacaaaagct ccggacagcg
    22921 tctcggtggc cttcgccacg gatccaaacg gcccctatgt gtcgaacccg gcgaatccgc
    22981 aggggatcaa gtatgtccaa gtagtggcta acggaagcgt gaccctctac ttcctgccgg
    23041 cgttcgccaa gaccgcccct ttgagcatgt tgtggaccat cggcggcaac cagtccatga
    23101 ccgccagcgc cgcctccggc cagtttctca agtccaccgc attttccacc ggtttgcttc
    23161 cgtattcccc gtatgcgcac aatcccaacg acccgaactt tggcttcacg gtcggggagc
    23221 aatacacgct gcgatggccg ccgccaggcc agcaaaagaa gggcagttgc ccgggcgacg
    23281 ttgggttccc ggacccatcg aactcagctt cccagcgggg cttcattgat atcgggtggt
    23341 cacagggaag cggatctgcg tcttttatcc gcgaagccat catcagcggt gtgcagtcga
    23401 agccgctgaa tgtcggcgac attctcaaca tggtcactgg gaacaagggg acggaatcag
    23461 atgccctgca agaacggtat aacgaagaca cagatacgac ctcggattat cggacttatc
    23521 tgacggactt gcagaacggg acggccaatg gccgacgcct gggaatcgtt ccgatcaacg
    23581 atccaagtca gaatgacaaa gtcctcggct ttgccttggt tcttcttccc gccaatcctt
    23641 gcggctcgaa aagcacggat ccgtgctgcg ccgtttacct agggaacaat ccggttctgc
    23701 ctgctttgcc aggaggagcc acgacccccg gggcctatca gttgaagctt aacaagtaat
    23761 gcccgggcgc caggatttga gcggataaga ccagcgtaag gcggcgtagg aaaaacagct
    23821 taacgcttcg cgtctaaaga taggttggct ccggccgcag gatcgtctat cctgagcgcg
    23881 aaaagtgttg cactccaggg ttatcgggag ggggaatgcg ctggaatatt tcttcatgct
    23941 cgcgggcgaa atcgggcttc gtcctgataa cggttgcgat aaccatcgtc gcgctgctcg
    24001 gcattttggg aatgtgtgtg gatttgtctc gcatgtacat aaccaagaac gagctgcagg
    24061 cctatgcgga cgcggcttcc ctggccgccg cacaccattt ggatggcacc tcgctgggca
    24121 tcaccaatgc aacaacgatc gccaagaact atcccgacgc ttacaacttc gcgacgacaa
    24181 agcctgacag cgtgagtgtg gcgttcgcca gcgaccccgc cggcccttac gttacaaatc
    24241 cagcaaaccc gcaaggcatc aaatacgtgc aagtgacggc gaatggctcg gtgaccctgt
    24301 atttcctgcc tgcgttcgcc aagactgcgc cgctaggaat gctgtggagt atcggcggca
    24361 gccagtcgat gatcgcgatc gccacgtcag gccagtttct taagagcaac gcattcaaca
    24421 caggcctgct tccctattct ccttacgttc acgatcccac cgacactacg aactatggct
    24481 tcgttcccgg caggtcctat acgctgcgct ggccgccgcc ggggcaggag aacaagaaca
    24541 acgcttgtcc ggccgaagtg acgcagaatt tttcggatcc ttccagctcg aattcccagc
    24601 ggggcttcat cgacatcggc tggccggctg gcaatggaaa tggaggccca ggcggcggat
    24661 cggcgttcat ccgcgaagct attatcagca atgtgcagtc caagccgctc ggattgggcg
    24721 atgacgtaaa tttcgtcagc ggcaaccggg gaactgaatc cgacgccttg caagagagat
    24781 ataccgaaga catcgatacg accgccgact ggcagacgta catgactgac ctgaacaacg
    24841 gcacggcgaa cggccgccgc ttgggcattg ttccgatcaa cgacccgaat tccagcccgg
    24901 ccggaaaggt cgttgccttc ggcttgttct tactgcaaaa caagccgtgc ggcaacagca
    24961 gcgtcgctcc ttgctgcgcg acattcatcg ggatgaatcc cattctcccg ggcggcacag
    25021 ccggcaccag cacgccgggc gtgtatcaat tgaagctgtt caagtgaaga gaggggcggt
    25081 cctgcgccgt cagcattccg gcggcaagcc cgcggcggtc aggcgtgatt gccgtgagag
    25141 caaccgaagg aagccgggac ccggccgccc ttgttcagtt cgtcataacc gatttttgaa
    25201 gtgtaatagc ctgccgctgt ccgtcgcttg atgtcttgaa agaaggcgac gcccgtatgc
    25261 agctccgccg gcgggttcgc ggcgggatct aggcttcgca gcagttccac ttgttgtgcg
    25321 gcggagcagc gaacgaaagg cttcccattc tgccgcagag cgtatccatc cagccaggcc
    25381 agcccctgta agaacgtgcg ccgcgcctcc ggagacgact cacttagcat caagtcgatg
    25441 aaacggttta cctgagcttc cttcgctcct ggagtgtcgg tcgccggaat gatcaactcc
    25501 gtcaaggcga cgaccgtatc gttctggtgc gcgtcaaaaa cgcgcggctg ccacggacca
    25561 gcccccgccg cgccgcggtc gaggctagtt tcgttccgcc cttggcctgc ctgttccacc
    25621 tgaggcaaag gctgaggcgg agccggcgct gctcgcagaa cggcggcgcc cgccccggtg
    25681 atggctccct ttaaaacttg ccggcgattc attgttttgc tccctgccgt gcccgcaact
    25741 ccgactgcgc agcgcgatag gaactgcgca ccaaaggtcc cgcctccaca tgggcaaagc
    25801 ccagctcaag cccagcgcgg cgaaatgcct cgaactcccg gggctcgtag tatctcacaa
    25861 tcgggagatg gcggggtgac ggccgcagat attgccccag cgtcaggata tcgaccccac
    25921 atcgcgctaa atcgcgcatc actgccagaa tttcggcgcc ggtctcgccg agacccagca
    25981 tcattccgga cttgacgggc atctccggcg ccatccgttt ggcgccggcc aggatgtcca
    26041 gcgacctttc atagcgcgcg cccggcctca cctgtcgata gagccggggg atggtttcaa
    26101 tgttgtggtt gagaacgtca ggacgcgcct ccaggacggt gtgcagggct tcgcgggaac
    26161 cctgaaagtc aggaatcaac acctcaatac ggcagccggg cacctgctcc cggacggctg
    26221 cgatcacgag ggcgaacaac tcagctccgc catccttccg gtcgtcgcga ttcacgctgg
    26281 taatcacggc gtaccgcagc cccaaggcct tggccgcttc ggccacgcgg cgcggctcgt
    26341 cgtagtccac cacgagcggg gcgcctttct gcacggcgca aaagccgcac cggcgcgtgc
    26401 atacgtttcc gaggatcatg aaggtcgctg tgcgggcgtt ccagcattcc ccaatattcg
    26461 ggcaagccgc gctctcgcag acggtatgca ggttcagccg ggttaccagt gcttttagct
    26521 cccggtagtt ctctccggcc ggggccgggg cttttagcca cgggggacgc ggcgcgaggc
    26581 cttcaatcgt gactagagga ctcatggtac aaatggtaat tggcccactc tttgagggcc
    26641 actgccatta ctgtagcgca gccggctggg gtgcggcgcc ctgcacatgg ataccttgac
    26701 tgtacaattt ctaggcgctg ccgggaccgt taccggttcg cgccacttgc tttccatcgg
    26761 gaagagccat ttactggttg attgcgggct gtttcaggga cgcaagagcc tccggcttca
    26821 aaactggatc cctttccctt ttcccccggc ggacctcgaa ggcatcgcac ttacgcatgc
    26881 ccacctcgac cataccggct acttgccgag actggtaaaa gaaggttttc gcagtccggt
    26941 ttactgttca gagccgacgg ccgatctgct cggtgttctt ctgccggatt ccggccacct
    27001 tcaagaagaa gaagccgagt ttgccaacag gcgaggctat tcgaaacaca aaccggctct
    27061 ccccctttac acggccgaag acgccgagga cgcgttgaag tatgtccgtc ctgtgccccc
    27121 cggccagccg gttcgtatgg agaacggatt tcaattccat ttccacaact ccgggcatat
    27181 tttgggatca aaggccattc acgtgatcat caatggcctg cgcattctct tcaccggcga
    27241 tctgggccgg tttcgagcta cgggagggaa gcctgacgcc attcccgaca gcgtagatta
    27301 cctgatcatg gaagcaactt atggcgaccg ccttcatcct aaagatgacc cgcgcccgcg
    27361 gcttgccgaa attatccgag atacggcgaa gaagggcggc gccattgtga taccggcgtt
    27421 tgccgtcgag cgtacgcaga agctgctgtt cattctgcgg ggcttgatgg acgaagggct
    27481 gattccgcag atccccatcc acgtcgatag cccgatggcc atagaagcag tgaacatttt
    27541 cctgcggcac agtgaagaat atgatgacgg gaccaaggcc atgatcaaac gtcacggcag
    27601 cccgctcaaa tggcccgatg tgcacttcga taaaacaatc gagcaatcca agcaggcgag
    27661 cgcaagcagg tctcctgcca tcattatctc cgccagcggc atggccacag gaggacgcgt
    27721 cttgcaccac cttgcgcagc ggctgccaga ccatcgcaat accgtgctct tcgtgggctt
    27781 ccaggcgccg gatacccggg gtcacctgat cgcttccggc gccgccacgg tgaggattca
    27841 cggccaggag gtgccggtga gggcgcgtgt cgagagcttc gaagaattca gcgatcacgc
    27901 cgactatgag gagattctgc gatggctgtc gtcgtttcgc tcggcgcccc gccaggtctt
    27961 actggtgcac gcggagccgc cggcagccca cgcgctggag cagcggattg aatcgaagtt
    28021 cggctggcct gtccatgtag cggagcaggg cgagcgggtg gcgctggact gagcatgtac
    28081 gacgttctca ttattggcag cggaccgggt ggctatgtag ctgcaattcg agcgggagag
    28141 tacggcttaa aggtgggggt catcgagaag gacccgtacc tgggaggcgt ctgcctccac
    28201 gtcggctgca taccgaccaa gtcgctgctt tacagcgcgg aaatatacga ccactttctc
    28261 caccccggcg agttcggaat ttctctcgac aatctgaagc tcgactggag ccgggtgcaa
    28321 tcgagaaaag aaaagacggt cgagaagcac gcaaaaggcg tggaatttct cttcaagaag
    28381 tataaggtgg agacgattcg cggctacggg cgcgtgaagg ccgccgggca agtggaagtg
    28441 gagggcccca acggcaaaca ggatctcgct gccagaagca ttatcctggc gacgggatcg
    28501 gaagcccgca tgctgccggg attgcagccc gacccggagc ggattctgac caacgttgaa
    28561 atcctgaagc tggcgagcat accccagagc ctggcgatca ttggagccgg cgcggtggga
    28621 gtcgaattcg cgtcgattta ccgccgtttc ggcagcgagg tgaccattat cgaaatgctg
    28681 ccgcgtctgg ttccactcga agatgaagag gtctcggcgg agcttggccg cgcgtttcga
    28741 aaacagggga tcgcgatgta caccggcgct cgcgtcgagc gcgtcgagaa gcggcccgac
    28801 cgcgtggcct tggagttccg ggactccggc ggaactacgc agaaactgga agccgagaag
    28861 ctcctggtgg ccgtcggacg caaacccaac accgaaaaca tcggcttgga aaagacggcg
    28921 atcaagaccg accgcggctt cgtgccggtg gatccgtgga tgcgcaccgc cgagcctgga
    28981 gtgtatgcga tcggggacat tgtcgcgggg agcccacagc tcgctcacgt tgcctccatg
    29041 gagggcctcg tggcggttgg ccacattgcg ggaaagcccg tgcgtccggt gaattatcgc
    29101 cggattccgt cttgcactta ttgcgagcct caaatcggca gcgtgggact gacagaggcg
    29161 caagccaaag cagagggccg ccaggtcaaa gtcggcaaat tcggctttgg agccaatagc
    29221 aaatcgtcga tcattgggaa tcattccggt tttgtgaagg tggtttcgga cgcgcagtac
    29281 ggggaaattc tgggggtaca catcatcggg ccttcggcga cggaactgat tgccgaggcg
    29341 gtggccgcga tggaatcgga ggctaccgtg gaatccatga tggcgactat ccacgcccac
    29401 ccgacgcttt cggaagccgt ttgggacgct tttaactccg tttacggttt gaccatcaat
    29461 gcctgaaccg ccgactcttg agtgccgctg ggccgatctc ggacgaatgg attacggcgc
    29521 tgccttcgct ttgcaacaaa ggctcgtcga gagccggcgc cggggcgaaa tcccggattg
    29581 gctgctcttg cttgagcatt cgcatgtcat caccctcggt cgcaacggca agcgc
//
LOCUS       DASREK010000175        11906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_87863, whole genome shotgun sequence.
ACCESSION   DASREK010000175 DASREK010000000
VERSION     DASREK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87863"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFA54_09065"
     CDS             complement(<1..377)
                     /locus_tag="VFA54_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZS50996.1"
                     /translation="MSAAATQEPTFVRQLLGRSDYAIISELVEPGSKVLDLGCGDGEL
                     LAWLVENKGVTARGVEISPARVQQCIMQGLSVYQGDIDAGLADYPDQCFDFVILSQTL
                     QETHHPLRVLREMLRVGRRIIVA"
     gene            complement(374..1480)
                     /locus_tag="VFA54_09070"
     CDS             complement(374..1480)
                     /locus_tag="VFA54_09070"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZS50997.1"
                     /translation="MIVETQCAHFDSLPLDCGETLSPVTVAYETYGNLNASRSNAILI
                     LHAFSGDAHAAGVSRENGKLGWWDSMIGPQKAFDTDRYFVICSNVLGGCRGTTGPSSL
                     NPATGRAYGSAFPVVTIPDMVRLQKKLVDYLGISRLLAVAGGSMGGMQALQWAVAYPE
                     AVASAIPIASAYRHSAQQIAFNEVGRMAIMSDPDWNGGDYYESGPPARGLSVARMIGH
                     ITYMSDESMREKFGRRQRPKDELRNGFDVDFEVENYLRYRGGEFVNRFDANSYLYITK
                     AMDNFDLSSGYGSLSGALARTKARFLVISFTSDWLYPSYQSQEIVRALRGHNLDVAYC
                     ELQSNYGHDAFLVEVGEQTELVRHFLANTFKKAA"
     gene            1707..2255
                     /gene="hpt"
                     /locus_tag="VFA54_09075"
     CDS             1707..2255
                     /gene="hpt"
                     /locus_tag="VFA54_09075"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZS50998.1"
                     /translation="MNDSELRPLISAEQIRARVQEMGRQIQTDYPEGPLRLIAILKGA
                     CFFLSDLARAIERDVSIDFMGIASYGRGKSSSGEVKVTKDLDNEIAGLDVLIVEDIVD
                     TGITLAYLCQVLQQRKPKTLRIATLLDKPSRRLKPVDLAYVGFQIPDEFVVGYGLDYA
                     ERYRNLANICVLAEGPGEGAPR"
     gene            2473..3435
                     /gene="deoC"
                     /locus_tag="VFA54_09080"
     CDS             2473..3435
                     /gene="deoC"
                     /locus_tag="VFA54_09080"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HZS50999.1"
                     /translation="MTTAQRCQGGRLFTATQVRDALCNQCQLLFLGGPTQATGQNPGD
                     RTAHPNVETSEIDRLAEQIYREMLARFGPPSERRFARDQASASPKLSFEQRVASLIDH
                     TLLRPDATREQIEELCRQALHFGFASACVNPVWAPVCAKLLHGSGVRVCTVVGFPLGA
                     TLTEVKRFETGQALKLGAEEIDMVIPVGALRSGDLDRVYLDILGVVEAARAARAVVKV
                     IIEAGLLNDSEKIAASTLAMLAGADFVKTSTGFGPSGATVRDVEILRRVARGAMGVKA
                     AGGVRSFGDFKRMIAAGATRIGASASVRILQEAKEAAEQEEPAL"
     gene            3432..5174
                     /locus_tag="VFA54_09085"
     CDS             3432..5174
                     /locus_tag="VFA54_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZS51000.1"
                     /translation="MTSRAPTLFKQRSDLLDFLLEVSEISSETLDLDRLLPALAKLIK
                     KVIDYEIFAILLVGDGGLMRIRFTIGHRQEVVEHLRIPIGQGITGAAAASRKPVLVND
                     VRKDPRYLNALDPVRSELAVPMIARGKVVGVIDIQSTHLDAFSEQEQAIVELIAGRVA
                     MAIDNARLYRRVLRQNKTLRTLSAIAQEFSSILELDPLLHKISEKVRQVINYDAFSIL
                     LLEPSESMLKDYMSVRYDRRVRLDNIPLGRGIIGAAAEFKQPILVPDTSKDPRYIAHD
                     PGIRSEVAVPLLVQNRLLGVLDLESEQLNYFTEEHVRTLSLLAPQIATAIENARLYQR
                     VARSEARIKKDLEAAWQLQQHLLPGGCPAVEGLDVAARLEPARQIGGDLYDFAPFSDG
                     RLAIAIGDVSGKGAAAALYGALVIGLLRLLAEQHHSPAPLLKALNHALLDRKIDSNYL
                     TLLCSRWDPGARTLVMANAGMPLPILLRDGVAAEQRVEGIPLGLLHDTDYEEVELPLK
                     SGDIVVFTSDGIIDGRDQFGQEYGRQRLTELIEKNGDLPAAGLAQKIFQDAQNHAAGA
                     PVFDDETIVVLKVR"
     gene            5184..6437
                     /gene="lysA"
                     /locus_tag="VFA54_09090"
     CDS             5184..6437
                     /gene="lysA"
                     /locus_tag="VFA54_09090"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZS51001.1"
                     /translation="MPTLGFEYKSGLLHCDEVSLAEIAGQAGTPCYVYSARSILKNYD
                     AYAQVRGPAPHTVHYSVKANGSLGILSLLARHGAGFDIVSGGELFRVMQAGGDPSRVV
                     FSGVGKTKNEIDYALGAGIALFSCESEEELDLIHERAERRQIRAPVALRVNPDVSAET
                     HPYIATGLREHKFGVPLAEAPALYARAAHMKNLSIEGVSCHIGSQIFDLRAFEEALCK
                     ILDLVDALHGQGIFVRHLDLGGGLGVGYRRDEPGPLIAEYMSALWKQLHGRDLHLHIE
                     PGRSIVAQAGVLLTTVLYRKRSASKEFVIVDAAMNDLIRPALYKSYHDIIPVICFDRP
                     AVKADIVGPVCETGDFLARDREMPAVETGDLLAVCTAGAYGFVLSSNYNARPRAAEVL
                     VDGAAFTVIRERETYADLIRGERTL"
     gene            6439..7458
                     /locus_tag="VFA54_09095"
     CDS             6439..7458
                     /locus_tag="VFA54_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZS51002.1"
                     /translation="MLRYLLTIGFTLLLPGLCPARSHQVIATEYYHTFSKTNPPLLHI
                     QPGDIVFTKTIDSGGQDYQGVRRSPLGVSNPLTGPFYVEGAEPGDAVIVHFRRVALNR
                     NWGYTAFRLGLYALTPDYVATVYPDNYKQDLVRPGRSNLVPWDIDIQRKVVRLREPAS
                     QRAKMEFPAELMLGCVGVAAADDFAPTSSISGPYGGNLDYNRIGEGATVMLPVFHPGA
                     LLYIGDGHALQADGEPTGTAVETSLDVEFSVDVRKKAGLSGPRVENDEYLISIGSQPE
                     FASSLDRALQMATTDMVQWLTKDYRLEPWAAHLMVGYEGRYDVVTVAGSMAVRIPKKY
                     LPPPR"
     gene            7487..8698
                     /locus_tag="VFA54_09100"
     CDS             7487..8698
                     /locus_tag="VFA54_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZS51003.1"
                     /translation="MNLRSNLASALAGARAQTDRLFRLVRVEALYERPVPERHRLIFY
                     LGHLEAFDWNLICAGKLEMPRFHPAFDRLFAFGIDPPPGQSPADRPSDWPSAEEVKAY
                     GHQVRERLDQVLVQAPEECIHAAIEHRLMHAETFAYLLHSLPVEQKLPQAPEGGSGQP
                     APHPQMIEIPSGKAILGRPRGDGFGWDNEFVEHAVEAPAFAVGKYKITNHQYLQFVED
                     GGPAPHFWKQDGRQWRYRTMFGEIPLPLSWPAYVTYAQAAQYAAWAGKSLPTEAQFHR
                     AAYGGENERTRPYPWGAAPPNATRGNFDFARWNPLPVDATPAGDSALGVSQLVGNGWE
                     WTSTVFHPFPGFQPLPFYPGYSADFFDGAHFVLKGGSARTAAVLLRRSFRNWFRPGYP
                     HAYAGFRCVEN"
     gene            8702..9670
                     /gene="egtD"
                     /locus_tag="VFA54_09105"
     CDS             8702..9670
                     /gene="egtD"
                     /locus_tag="VFA54_09105"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HZS51004.1"
                     /translation="MATFPLVHPVEPNFAREVRAGLTKLGQKELPSMYLYDEVGTALF
                     EAITWLPEYGLTRADERLLRMHAEAMIDCLEPPVLVAELGSGTGRKTRYLLEALIRRQ
                     PVVYHPIDISRLALAKCRLELAGLGSIGIVGVEQSYLDGLRAVASWRRENERLLILFL
                     GSNIGNFERPAEERFLAEIRRCLSPGDGLLIGMDLEKPVPEMLLAYDDPAGVTAAFNL
                     NLLARVNRELGGDFNLREFAHEARYNQKQRRIEMHLRSLRRQTVSVAAANVACTLLEG
                     ETIWTEACHKFRLEEIPLMARRTGFACRAQWVDPQWPFAESLWIAN"
     gene            9805..10551
                     /locus_tag="VFA54_09110"
     CDS             9805..10551
                     /locus_tag="VFA54_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS51005.1"
                     /translation="MSAIVTFQNVSYAIGGKAILRDFSVEVEAGETLVLLGRSGSGKT
                     TALKMANAMMVPTSGEVWVEGKPTTAWDPIRLRRRIGYVIQDVGLFPHFTVAGNVGLV
                     PSLEGWPAAKIKARVDDLLEQVGLPPEQFALRYPRELSGGQRQRVGVARALAADPPLL
                     LFDEPFGALDPVTRLELQKLFIGLRRKLGKSAIFVTHDVREAMLVGSRIGLLHRGRLD
                     VLATPSEFARATGEEAQAFLACLNANVEVS"
     gene            10551..11168
                     /locus_tag="VFA54_09115"
     CDS             10551..11168
                     /locus_tag="VFA54_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS51006.1"
                     /translation="MPSGLLDEILNLSAEHLVLVLISISIATLVGIPTGILLTRRLAP
                     RRWVLGFASIMQTVPSLALFGFLIPLPFIGGIGKRTAIVALVLYALLPILRNTLTGIL
                     TVDPAVRESAIAMGMTGAQLLRQVEIPLAARTILAGLRVATVTTIGTATIAAAIGGGG
                     LGVFIFRGVASVDTTQILAGAVPAALIALFADGGLGWVERKLSPA"
     gene            11138..>11906
                     /locus_tag="VFA54_09120"
     CDS             11138..>11906
                     /locus_tag="VFA54_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZS51007.1"
                     /translation="MGGTKTFACVIFCCALAACSRSHTVTVGSKNFTEQVVLGEIAAQ
                     HLERRLGEKIDRKLNLGGTLLVHQALIHGQIDLYPEYTGTALTAVLKLPPLSDANEVL
                     NEVRVEYAKRWKIDWLEPLGFNNTFAMVVPGGAARGARLETLTDAARRGPPGGWALGA
                     GYEFQQRPDGLEGLLKTYHLPLKGQPKIMDLGLLYKALEQNQVDMIAANATDGMLSVL
                     DVRVLEDDRHYFPPYQAAFLIREETARAHPRLRRALAE"
BASE COUNT         2231 a         3670 c         3686 g         2319 t
ORIGIN      
        1 aatgccacga tgatccgccg tccaacgcgc agcatttcgc gcagcacgcg aagcgggtga
       61 tgcgtttcct ggagagtctg gcttagaatg acgaaatcaa agcattggtc ggggtagtcc
      121 gccaggccgg cgtcaatatc gccctggtag acggagaggc cttgcataat gcattgctgc
      181 acccgggccg gcgaaatttc cacgccccgc gccgtgacac ccttattctc caccagccag
      241 gccagcagct cgccatcgcc gcacccaagg tccaacactt tgctgcccgg ttcgaccagt
      301 tcgctgatga tcgcgtaatc actgcgcccc agcagttgcc ggacgaatgt cggttcctgt
      361 gtagccgctg cgctcatgcc gcttttttga acgtattcgc cagaaaatgc cgcaccagtt
      421 cggtctgctc gcctacctct accaaaaacg catcgtggcc gtaattggat tgtagctcgc
      481 agtaggccac atccagatta tggccgcgca gggcgcggac aatctcttgt gactgatagc
      541 tcggatataa ccagtccgag gtgaagctga ttaccagaaa gcgggccttg gttcgcgcca
      601 gcgctccgga gagcgatcca taaccgctgc taagatcgaa attatccatg gctttggtga
      661 tgtagaggta cgaattcgcg tcgaagcggt tcacaaattc cccgccccgg taccgcaagt
      721 agttctccac ctcaaaatcc acgtcgaagc cgttgcgcag ctcgtctttc gggcgctggc
      781 ggcgtccgaa cttctcccgc atcgactcgt cgctcatgta ggtaatgtgg ccgatcatgc
      841 gggccaccga aagcccgcgc gccggggggc ccgattcata gtagtcgccg ccgttccaat
      901 ccgggtccga catgatggcc atgcggccga cttcattgaa cgcgatctgc tgagcggaat
      961 gccggtaggc ggaagcgatg ggtatggcgc tggcgaccgc ctccgggtag gcgaccgccc
     1021 actgcaacgc ctgcatgccg cccatcgagc cgccggccac ggccagcaga cgggaaatgc
     1081 ccagatagtc caccagcttc ttttgcagcc gcaccatgtc gggaatcgtc actacgggaa
     1141 acgcagaccc gtaggcgcgg cccgtcgccg ggttgagcga actgggcccg gtcgtgccgc
     1201 ggcatccgcc gagaacgttc gaacagatta cgaaatagcg atcggtgtcg aaggctttct
     1261 gggggccgat catgctgtcc caccacccca gctttccatt ttcacggctt acgccggcgg
     1321 cgtgcgcatc gccggaaaat gcgtggagaa tcagaatcgc attggaccgg gaggcgttta
     1381 agttgccgta ggtctcgtaa gcgaccgtca ccggggacaa ggtctcgccg cagtccagcg
     1441 ggagagaatc aaaatgcgcg cattgtgttt caacaatcat gccccgcttg cgcccgccct
     1501 ccgccctctt tccccgccgg gactcaggcg ggaagccatc cacagtacta gaatagcgtg
     1561 acctcttcgc ccaaggcaaa actcgacggt aaagtttccg cacgcgcggc ggcgggcgag
     1621 ggccgcgagc cggattgccg cgttggagat catcgcgccg cgcgccgcgg cgtggagaaa
     1681 aatttatttc ctgctacagt agcggcatga atgacagcga actgcggccg ctcatcagcg
     1741 ccgagcagat tcgcgcgcga gtgcaggaaa tgggccgcca gatccagacc gactacccgg
     1801 aaggacctct gcggctcatc gccattttaa aaggggcttg tttttttctt tccgatctcg
     1861 cgcgggccat cgagcgcgac gtctctatcg atttcatggg catcgccagc tatggccgcg
     1921 gcaaatcaag ctcgggcgag gtcaaggtga ccaaggatct ggacaacgaa atcgccggcc
     1981 tcgacgtgct gatcgtcgag gacatcgtag ataccggcat tacactggcg tacctgtgcc
     2041 aggtgctgca acagcgcaag cccaaaacgc tgcggatcgc aacgctgctg gacaagccca
     2101 gccgccggct caagcccgtg gacctggctt acgtgggctt tcagattcct gatgaattcg
     2161 tcgtgggcta cggtttggac tacgcggaaa gataccgcaa tctggccaat atttgcgtgc
     2221 ttgccgaggg gcccggggag ggcgcgcccc gatgaatgaa atacagcgag acgcaaagcc
     2281 tgttttccgg cgcggcgctg ctgggcggcg cgcttgggag cgccagaagt gcacggattg
     2341 ggcggatatt ccaggtcccg cggccaagga cagccgttcg gaaacagcgg cggcctgccg
     2401 ccaaagctct ctttagagcc agcaggtctc cttgtgttat gataaaactt ccgccaaaaa
     2461 cagccgctgc aattgactac ggcacaacgc tgccaggggg gacggctgtt taccgcaacg
     2521 caggtccggg acgccctgtg caaccagtgt caactgctct ttcttggagg gcctacgcag
     2581 gccaccggcc aaaaccccgg agatcgaacg gcgcacccca acgtggagac tagcgagatc
     2641 gacaggctgg cggagcagat ttaccgggag atgctggcgc gcttcggtcc gccttctgag
     2701 cggcgtttcg cccgcgatca ggcttcggcc agcccgaagc tctccttcga acaacgcgtg
     2761 gcgagcctga tcgaccacac gcttctgcgg cccgatgcga cgcgggagca aattgaagaa
     2821 ctctgccggc aggctctcca tttcggtttt gcgagcgcct gcgtcaaccc ggtttgggcg
     2881 ccggtgtgcg ccaaactctt acacgggtcg ggagtcagag tgtgcacggt cgtgggtttt
     2941 ccgttgggcg cgacgctcac cgaggtgaag cgcttcgaga ccgggcaggc gctcaagctg
     3001 ggagcggaag aaatcgacat ggtgatccca gtgggagcct tgcggtcagg tgatttggac
     3061 cgggtatatc tcgatatact gggcgtcgtg gaagcggcgc gcgccgcccg ggccgttgtc
     3121 aaggtaatta tcgaggcggg tttgctgaac gattcggaga aaattgcagc ctccacgctc
     3181 gccatgctgg caggagccga tttcgttaaa acgtccacag gcttcggccc ttccggggcc
     3241 acggtccgcg atgtggaaat cctgcgccgc gtggctcgcg gcgctatggg cgtcaaagcg
     3301 gcggggggcg ttcggagctt cggcgacttc aaaagaatga tcgccgcagg ggccacgcgc
     3361 atcggcgcca gcgccagcgt ccggattttg caggaagcca aggaagctgc ggagcaggag
     3421 gagccggccc tatgacttcc cgcgcgccga ctctgttcaa acagcgcagc gatttgctgg
     3481 attttctgct cgaagtgtcc gaaatttctt cggaaaccct ggatctcgac aggcttcttc
     3541 cggctctcgc caagctcatc aagaaagtta ttgattacga gatcttcgcc attctgctgg
     3601 tgggagacgg cggcctgatg cgcatccgct tcaccattgg ccaccggcaa gaggtcgtcg
     3661 agcacctgcg gattccgatc ggtcagggga ttaccggcgc ggctgcggca tcgcgcaagc
     3721 cggtgctggt caacgatgtt cgcaaggatc cccgctactt gaacgctctt gatccggtcc
     3781 gctcggaact cgccgtgcct atgatcgcgc ggggcaaggt tgtcggcgtg atcgacatcc
     3841 aatccacgca cctcgacgcc ttcagcgagc aggagcaggc catcgtcgag ttgatcgcgg
     3901 gccgggtggc aatggccatc gacaatgcgc gcctttaccg ccgcgtgctg cgccaaaaca
     3961 agaccctccg gacgctttcc gccatcgcgc aagagttttc ctcgatcctc gagctcgatc
     4021 cgctgttgca caagatttcg gaaaaggtgc ggcaggtgat caattacgac gccttcagca
     4081 tcctgctgct ggagccttcc gagtccatgc tcaaggatta tatgagcgtt cgttatgatc
     4141 ggcgggtgcg cctggacaac attcctctcg ggaggggcat catcggcgcg gccgcggagt
     4201 tcaagcagcc gatcctagtg cctgatacca gcaaagatcc gcgctacatc gcccacgatc
     4261 cgggcatccg ctcggaggtg gccgtgccgc tactggtgca aaaccggctg ctgggcgtgc
     4321 tcgacctgga gagcgaacaa ctgaattact tcaccgagga gcacgttcgc acgctttcgc
     4381 tgctggctcc ccagatcgcc actgccattg agaacgcccg gctctaccag cgcgtcgcca
     4441 ggagcgaggc gcggattaaa aaagatctcg aggcggcctg gcaactccag cagcatcttc
     4501 tgccgggcgg ctgcccggcc gtggaagggc tcgacgtcgc cgcccggctc gagccggcgc
     4561 ggcaaatcgg cggcgacctc tacgacttcg cgccgttttc cgacggccgc ctggcgatcg
     4621 cgatcggcga cgtaagcggc aaaggagcgg cggcggcgct ttatggcgcg ctcgtcatcg
     4681 gcctactccg ccttctggcg gagcagcacc actcgcccgc gccgctgctg aaggccctca
     4741 accacgcact gctcgatcgg aagattgact cgaattactt gaccctgctc tgttcccgct
     4801 gggatccggg cgcccggacg ctcgtcatgg ccaacgccgg aatgccgtta ccgatcttgt
     4861 tgcgggacgg agtcgcggcc gagcagcgcg tcgaggggat tccgttggga ttgctccacg
     4921 ataccgacta cgaggaggtt gaactgccgc tgaaaagcgg cgacatcgtg gtgttcacca
     4981 gcgatggcat catcgacggc cgcgatcaat tcggccaaga atacggccgg cagcgcctca
     5041 cggaacttat cgagaaaaac ggggatttgc cggccgccgg tctggcgcaa aagatctttc
     5101 aggacgcgca gaatcacgcc gccggcgctc cggtctttga cgacgagacc attgttgtgc
     5161 tgaaggtccg ataaacatcc ctcatgccga cgcttggatt tgaatacaaa tcaggactgc
     5221 tgcactgcga tgaggtatcg ctggccgaga tcgccggcca ggccggcacg ccctgctacg
     5281 tctattcggc gcgctcgatt ctcaaaaatt acgacgcata cgcccaggtt cgcggccctg
     5341 ctccgcacac cgtgcattat tcggtgaagg ccaacggctc gctcggcatt ctcagcctgc
     5401 tcgcgcggca tggcgcgggc ttcgatattg tttcgggcgg cgagctgttt cgcgtgatgc
     5461 aggccggcgg cgacccgtcg cgcgtggttt tctcgggcgt ggggaaaacc aaaaacgaga
     5521 tcgattacgc gctgggagcg ggtattgcgc tgtttagttg cgagtcggaa gaggagctcg
     5581 atctgattca cgagcgggca gagaggcggc aaatacgagc acccgtcgcc ctgcgggtca
     5641 atcccgacgt gagcgccgag acacatccgt atatcgccac cggtttgcgc gaacacaaat
     5701 tcggcgtgcc gctggccgag gccccggcgc tttatgcgcg cgctgcccat atgaaaaatt
     5761 tgagcatcga gggcgtaagc tgccacatcg gttcgcagat cttcgacctc cgggcattcg
     5821 aggaagcgct ttgcaaaatc ttggatctgg tggacgcctt gcatgggcaa ggcatcttcg
     5881 tccggcacct cgatttgggc ggcggcctgg gagtcggcta tagacgggac gagcccgggc
     5941 ctctgatcgc cgagtacatg agtgctctgt ggaagcagct ccacggccgg gatctgcacc
     6001 tgcacatcga gccgggccgc tcgatcgtgg cccaggccgg ggtcctgctg acgaccgtcc
     6061 tttaccggaa gcgctcggcg agcaaggaat ttgtcatcgt ggacgctgca atgaacgatc
     6121 tgatccggcc ggcgctatat aagtcctatc acgacatcat tccggtcatc tgttttgacc
     6181 ggccggcggt caaagccgat atcgtgggac cggtctgcga gaccggcgat tttctggcgc
     6241 gcgaccggga gatgccggct gtcgagaccg gcgacctgct ggctgtctgc acggccggcg
     6301 cctatggatt tgtgctgtcg tccaattaca atgcaagacc gagagccgcc gaagtgttgg
     6361 tggacggcgc cgcctttacg gtaatccgcg agcgcgaaac ctacgcagat ctgattcgcg
     6421 gagaaaggac gctctgaaat gctgcgatac cttctcacaa tcggtttcac actgctcctg
     6481 cctgggctct gtccggccag atcccatcaa gtgattgcga cggagtatta ccacacattt
     6541 tcaaaaacaa atccgccgtt gctgcacatc cagcccggcg acatagtttt cacgaagacc
     6601 atcgactccg gcgggcaaga ctaccagggc gtccgccgca gcccgctcgg cgtctcaaat
     6661 cccctgaccg ggccgttcta tgtagaaggc gccgagccgg gcgacgcggt gatcgtccat
     6721 ttccgccgcg tggccctgaa ccggaactgg ggatataccg ccttccgcct tgggctctac
     6781 gctctgactc ccgattacgt cgccacagta tatcccgata attacaagca ggacctggtt
     6841 cgccccggcc ggagcaacct ggtgccgtgg gacatcgaca ttcaacgaaa ggtggtgagg
     6901 cttcgcgagc cggcgagcca gcgcgccaaa atggagtttc ccgccgagtt gatgctgggc
     6961 tgcgtgggcg tggcggctgc tgatgatttt gccccgacat cttcgatatc cggcccgtat
     7021 ggaggcaacc tcgattacaa ccgcatcggg gaaggggcaa ccgtgatgtt gccggtgttt
     7081 catccgggcg ctttacttta cattggcgat gggcatgctt tgcaagccga tggcgagccg
     7141 acaggcactg cggtggaaac ctcgcttgat gtggaatttt ccgtggacgt tcgaaaaaag
     7201 gccggcctgt ccggtcctcg tgtggaaaac gacgagtact tgatcagcat cggcagccag
     7261 ccggaattcg ccagctccct cgaccgcgcg ctgcaaatgg ccacgacaga catggtgcag
     7321 tggctcacca aagattaccg actggagccc tgggcggcgc atttgatggt cggctacgag
     7381 ggccgctatg acgtagtaac ggtcgccgga tctatggccg tgcgaatccc caagaaatac
     7441 ctcccgccgc cgcgttgagg cgtctaatta cagaggaggg ccgtatatga atttgcgaag
     7501 caatctcgct tccgctctag ccggcgcccg ggcgcaaacc gaccgattgt ttcgcttggt
     7561 tcgcgtggag gccctctatg agaggccggt cccggagcgc catcggctga ttttttatct
     7621 aggtcatctg gaggcctttg actggaacct gatctgcgcc ggcaaactgg agatgccccg
     7681 gtttcatccg gcgtttgacc gcctgttcgc tttcggcatc gatccgccgc ccgggcagtc
     7741 gccggccgat cggccttcgg actggccctc ggccgaggaa gtcaaagcct atggacatca
     7801 ggtgcgcgag cggctggatc aggttctggt tcaggccccc gaggaatgta ttcatgcggc
     7861 aatcgagcac cggcttatgc acgccgagac cttcgcttat ctgctgcaca gcttgccagt
     7921 tgagcagaaa ctgccgcagg ctccggaagg cggatccggg cagcccgctc cgcaccccca
     7981 gatgatcgag ataccctccg ggaaagcgat tttggggcgg ccgcgcggcg atggctttgg
     8041 atgggacaac gaatttgtcg agcacgccgt agaggcgccc gccttcgccg tcggcaagta
     8101 caagatcact aatcaccaat atctacagtt cgtggaggac ggcggcccgg cgcctcattt
     8161 ctggaagcag gacggccgcc agtggcgtta ccggacgatg ttcggtgaaa ttccgctgcc
     8221 gctgagctgg cccgcttacg tcacctacgc tcaggctgct cagtatgccg catgggcggg
     8281 caagagtttg ccgacggaag ctcaattcca tcgcgccgct tatggcggcg agaatgagcg
     8341 cacaaggccg tatccgtggg gagcagcgcc gccgaatgct acgcggggaa atttcgactt
     8401 tgcgcgatgg aatcctctgc cggttgacgc cactcccgcc ggcgacagcg cgctgggcgt
     8461 ttcccagctt gtcggcaacg ggtgggaatg gacctccaca gtgttccacc cctttcccgg
     8521 gttccagccg ttgccgttct acccaggcta ctcagccgat ttttttgacg gcgcccattt
     8581 cgtactgaaa ggcggttcgg cccggacggc ggcggtgctg ctgcggcggt cgtttcgcaa
     8641 ctggttccgc ccaggttacc cgcacgccta cgcgggtttt cgctgcgttg agaattgaaa
     8701 tatggcaact ttcccgctgg ttcacccggt ggagccgaat ttcgcgcggg aagttcgtgc
     8761 aggcttgacc aagcttggcc agaaagagct gccgtcgatg tatctctacg acgaggtcgg
     8821 cacagcgcta tttgaagcaa tcacgtggct tccggaatat ggactcactc gcgccgatga
     8881 gcgcctgttg cgaatgcacg cggaagcgat gatcgattgc ctcgagccac ccgtgctggt
     8941 tgcggagttg ggcagcggca ctgggcgcaa gacccgctac ttgctcgagg cgctgatccg
     9001 ccgccagccc gtagtgtacc accctatcga catctcccgc ctcgccctag ccaaatgccg
     9061 gctcgagctc gccgggctgg ggtcgattgg catcgtggga gtcgagcaat cttatctcga
     9121 cggcctgcgc gcggtcgcct cctggcgccg cgagaatgag cggttgctga tcctatttct
     9181 cgggagtaat atcggcaact tcgagcgccc ggcggaggaa agatttctgg ccgagatccg
     9241 ccgatgcctc tctccgggag acggcctgct gataggcatg gatttggaaa agccggttcc
     9301 cgagatgctg ctcgcctacg atgatccggc cggcgtcacc gcggctttca atctgaactt
     9361 gctcgcacgg gtgaatcgcg aactcggcgg ggatttcaac ttgcgggagt ttgcccacga
     9421 agcccggtac aaccaaaagc agcgccgcat cgagatgcat ctccgatcgc tccgccggca
     9481 aacggtttcc gttgccgccg ccaatgtcgc ttgcaccctt ttggaaggag agacgatttg
     9541 gacggaagcc tgccacaagt ttcgcctcga ggagatcccg ctaatggccc gccggacagg
     9601 cttcgcgtgc cgagcacaat gggtcgatcc gcagtggcct tttgccgaga gcctttggat
     9661 tgcgaactag cctctagcgc cggtaagccg gaaacagacg cgtccttcct tacagcaagc
     9721 cgttttgcgc tagcgcttgc agagatgctg gcggccggcg cccggcggct gtaagctgag
     9781 ggctgagccc tacggctgtt gcccatgagc gccatcgtca cctttcaaaa tgtatcctac
     9841 gcaatcggcg gcaaagcgat tctccgcgac ttcagcgtgg aggtcgaggc cggtgaaacg
     9901 ctggtgctgc tcggccgcag cggctccgga aagacgacgg ctttgaaaat ggcgaatgcc
     9961 atgatggttc ccaccagcgg cgaagtttgg gtggaaggca agcccaccac ggcctgggac
    10021 ccgattcgcc tgcgccgccg catcggctat gtgatccagg atgtcggact cttccctcat
    10081 tttaccgtcg ccgggaacgt gggcctggta ccaagcctgg agggctggcc ggccgcgaaa
    10141 atcaaggcgc gagtcgatga cctgctggag caggtagggc tcccgccgga gcaattcgcc
    10201 ctgcgctacc cgcgcgagct ttccggcggc cagcggcaga gggtcggggt agcccgggcg
    10261 ctagccgccg atccgccgct cctgctcttc gatgaaccct ttggcgcgct cgatccggta
    10321 acgcgtctgg aactgcaaaa gctcttcatc ggtctgcggc gcaagctggg caaatcggct
    10381 atcttcgtca cccacgacgt ccgggaggcc atgctcgtag gctcccgtat cggcctgctc
    10441 caccggggga ggctcgacgt gctggccact ccatcggaat ttgcgcgcgc cacgggtgag
    10501 gaagcgcagg cgttcctggc ctgccttaat gcaaacgtgg aggtgagctg atgccttccg
    10561 gtctgctgga cgaaattttg aacctcagcg ccgagcatct ggtactggtt ctgatctcca
    10621 tttccatcgc aacgttggtc ggcattccga ctggcattct ccttacccgc cggcttgcgc
    10681 cccggcgctg ggtgttgggc ttcgccagca tcatgcaaac cgtgccgagc ctggctctat
    10741 tcggctttct gattcctctg cccttcatcg gcggaatcgg caagcggacg gcgattgtag
    10801 cgctagtgct ttacgccctg cttccgatcc tccgcaacac cctgaccgga attctgaccg
    10861 tggacccggc ggtgcgcgaa tcggcgattg ccatgggcat gacgggcgcc cagcttctgc
    10921 ggcaggtgga gataccgctc gccgcccgca cgattctggc tgggctgcgc gtcgcgaccg
    10981 ttacgaccat cggcacggcg accatcgccg cggccatcgg aggaggcggc ctgggcgtct
    11041 tcatattccg cggcgtcgcc tccgtcgata ccacgcaaat tctcgccggc gcagttccgg
    11101 cggcgctgat tgccttgttc gctgacggag gcctgggatg ggtggaacga aaactttcgc
    11161 ctgcgtgatc ttctgttgcg ccctggccgc ctgctcgaga tcacacacgg ttacggtcgg
    11221 gtcaaagaac tttaccgagc aggttgtatt gggagagatc gcggcccagc atctggagcg
    11281 ccggctggga gagaagattg accgcaaact gaacctgggc ggcacgctcc tggttcacca
    11341 ggccctgata cacggtcaga tcgatctata tcctgagtac acaggtacgg ccctgaccgc
    11401 cgtgctgaag ctcccgcctc tctccgatgc gaacgaggtg ctcaacgagg tccgggtgga
    11461 gtacgccaaa cggtggaaaa tcgactggct ggaacccctg ggtttcaaca ataccttcgc
    11521 catggtcgtt cccggcggcg cagcccgcgg cgcccgcctc gagacgttga ccgacgctgc
    11581 ccgccggggc ccgccgggcg gttgggccct cggcgcaggc tacgaattcc agcagcggcc
    11641 cgacggcctg gagggattat taaaaactta tcatctcccg ctgaagggcc agccgaagat
    11701 catggatctt ggcctgctct acaaagctct cgagcagaac caggtcgata tgatcgccgc
    11761 aaacgccacc gacggcatgc tttccgtgct cgatgtcagg gtgctggagg acgacaggca
    11821 ctattttccc ccgtaccagg ccgcgtttct gatccgcgag gagacggcga gagctcatcc
    11881 tcgcctgcgg cgggctctgg ccgagc
//
LOCUS       DASREK010000176         4047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1075933, whole genome shotgun sequence.
ACCESSION   DASREK010000176 DASREK010000000
VERSION     DASREK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075933"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFA54_09125"
     CDS             complement(<1..657)
                     /locus_tag="VFA54_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZS51008.1"
                     /translation="MFRRLFFAASLCFSMQLAVPPPSAAQEWKVYGGDLSSTRFSPLN
                     QINTSNVNRLTVNWVFQTGVPGKFEATPLFENGILYFTGPGGYAWAVDARTGRALWNF
                     ERPIPSKIGLCCGPVNRGFALAGNRLITVTIDAHVIALDKRTGALLWDTTMADYTKGY
                     SATAAPLLVKDKVIVGMAGAEFANRGFIDAYSVATGERAWRFWTIPSPGEPGSETWKD
                     G"
     gene            complement(659..1444)
                     /locus_tag="VFA54_09130"
     CDS             complement(659..1444)
                     /locus_tag="VFA54_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS51009.1"
                     /translation="MASGQRLAAFAICAIFGGSLGNAQTKPNLGSTSVLFHRACAVCH
                     GLNGNGGRGPDLVSGRWSHGSTDADLARVITNGVPGTDMPAFGQRFNQEEITKLVAFV
                     RSLSAGGGNLKITGNAERGQEIYWGKGACSSCHMVNGQGGTLGPELSRIGAQRSPASL
                     KESIVSPSAAIVTGYQTVKVTQGGRTITGIRKNEDNYSIQVFDGNEYHSFDKSKIDRL
                     EEPPESLMPASNLSASEVDDLVAYLDTLRAPQQPMGVSRRGRR"
     gene            1443..>4047
                     /locus_tag="VFA54_09135"
     CDS             1443..>4047
                     /locus_tag="VFA54_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HZS51010.1"
                     /translation="MPRKTSLNSSYHSMHVRLGAHRKPAPFRPPALMKYGIISALLVS
                     FAVLVSVVLPADTVQERLAQHINLGKAFYENPGASRQAVEEFRQAMALAPNSAREHLN
                     YGLALLRAGRTSEGAAELEKARSLNPSLPHTYFNLGVVYKKEGDFTRALREFEQMARL
                     VPDEAVTHYNLGTLYKLQGQNEKAISEFETAARLDPALAAPHFQLFNAYRLAHNTEQA
                     QRELAAFQEIKNKQSAEGASEDMDWSYYSEILDDIHSGGASSEPAQPAEVVFQDQLLG
                     TIAGRDPGIAALDADGDGRLDLLAWSADRVVLFRNTGKGFSAFPVEGARGPAFAGDFN
                     NDGLPDLCILNPPGAELWTNRKGSFARTAKLPPAGSFRKALWVDFDHDYDLDLLLMGE
                     QQALLRNNGDGSWSDVSGQFPFAAGQALDAVALELEPNGNTFDIVAAYRNRPAVLYRD
                     RKAGRYEATPLAFPLEGALETGDFNQDGSLDVAAVGSARLAFIENRGGNLVRRPDVPL
                     AAGAGALFADFQNRGRVDVLGDRRMLLSNGEFTYQNGNVRGTTGGWLAAVAGDFNNDG
                     LTDAAVIRDGGSVHLLLNLTPKKNNWLKVALTGVKNLKLASGARVEVKAGGLYEKQVY
                     QGVPLVFGLGGYTFADTVRITWPNGLVQNEIRQKAGMSYTFKEAPRLSGSCPMIFTWN
                     GRRFEFISDVLGVAPMGAGLAEGEVFPLDHDEYVQIPGRALQPRGGLYDVRVTEELHE
                     VSYLDQIQLVAVDHPAGIEIFTNEKFKSPPFPDFRLFGVKRRMYPSAARDDHGRDVRE
                     LILRRDARYPDGFRRDLNGRAEMHSLTLDFPNLPAHPVLFLHGWVDWADGSTFVAASQ
                     SRAGSLVIPYLQ"
BASE COUNT          756 a         1243 c         1225 g          823 t
ORIGIN      
        1 cccatctttc caggtctccg agccgggctc gcccgggctg gggatcgtcc agaagcgcca
       61 ggctcgctcg ccggtggcca ccgagtaggc gtcgatgaag ccccgattgg caaactcagc
      121 gccggccatg cccacgatta ccttgtcctt caccaaaagc ggggccgcgg ttgcgctgta
      181 gcccttggta tagtcggcca tggtggtgtc ccacaaaagc gctccggtcc gcttatcgag
      241 agcaatcacg tgcgcgtcga tggtgaccgt gatgagccgg tttcccgcca aggcgaaacc
      301 ccggtttacc ggcccgcagc acaagccgat tttgctcggg atcggccgtt cgaagttcca
      361 aagcgcgcgt ccggtgcggg cgtcaacggc ccacgcgtat ccgccgggtc cggtgaaata
      421 caggatccca ttttcaaaca ggggagtggc ttcaaacttg ccgggaacgc ctgtctggaa
      481 cacccagttc accgtcaggc gattgacgtt agaggtgttg atctggttca gcgggctgaa
      541 ccgcgtgctg gaaagatcgc cgccgtagac cttccactcc tgagcggccg acggcggtgg
      601 aacggccagt tgcattgaga aacaaagcga agctgcgaaa aacaatctcc gaaacatgtc
      661 agcgtctccc cctccgactt acccccatgg gctgttgtgg cgctcgtaga gtatccagat
      721 acgcgaccag gtcatccacc tcgctcgcgg agagattgct ggcgggcatc agggactccg
      781 ggggctcttc caggcggtcg attttactct tatcgaaaga atgatattcg ttcccgtcaa
      841 aaacctgaat ggaataattg tcttcgttct tgcggattcc tgtgatggta cgcccgccct
      901 gggtcacctt tacggtctgg tagccggtca cgatggctgc cgagggagat acaatcgatt
      961 ccttcaggct cgccggcgag cgttgcgctc caatgcggct caactcagga ccgagcgtac
     1021 cgccctggcc attcaccatg tggcaggaag agcaagcgcc tttgccccag taaatttcct
     1081 gcccgcgctc ggcgttgccc gtgattttca gattgccgcc gccggccgaa agcgagcgca
     1141 cgaaggccac cagctttgtg atttcttcct gattgaatct ctggccgaag gcgggcatat
     1201 cagttcccgg aacgccgttc gtaatcacgc gggccagatc ggcgtccgtc gatccgtggc
     1261 tccagcggcc cgaaaccaag tccggcccgc gtccgccatt cccgttcagg ccatgacaca
     1321 ccgcacacgc gcgatggaaa agaacgcttg tgctccccag attcggtttg gtctgcgcgt
     1381 tgccgagaga tcctccgaaa attgcgcaga tagcgaaggc cgccagcctt tgaccgcttg
     1441 ccatgcctcg taaaacctcc ctgaattcga gttatcatag catgcatgtg cgcctcgggg
     1501 cgcaccggaa accggcgcct tttcggcctc cggcgttgat gaaatacggc atcatttccg
     1561 ctctgctggt ttctttcgcc gtgttggtct cggtggtctt gcctgccgac accgtccaag
     1621 agcggctcgc gcagcatatc aacctcggca aagcctttta cgaaaatccc ggcgcttccc
     1681 ggcaggcagt cgaagaattc cggcaggcga tggccctcgc tccaaattcg gcgcgcgagc
     1741 atttaaatta cggtctggcc ttgttgcggg caggccggac atcggaaggc gccgcggagc
     1801 tggagaaggc ccgttcgctc aatccttctt tgccgcatac ctacttcaat cttggggtcg
     1861 tctataagaa agaaggcgat tttacgcggg cgctgcgcga gttcgagcaa atggcgcggc
     1921 tggttcctga cgaggcggtc acccactaca atctgggaac cctctataag ctgcaaggcc
     1981 agaacgagaa ggccatttct gagtttgaaa cggcggcgcg cctcgacccg gctctagccg
     2041 caccccattt ccagcttttc aacgcgtacc ggctcgcgca caacacggag caggcccagc
     2101 gggaactggc ggcgtttcag gaaataaaaa ataagcaatc ggcggaaggc gcctccgaag
     2161 atatggattg gtcctattat tcggaaattc tggatgacat ccactcgggc ggcgcatcct
     2221 ctgagccggc ccaacccgcc gaagtggtgt ttcaggatca gcttttggga accatcgccg
     2281 gacgcgaccc gggaatcgct gcgctcgatg ccgatggaga tgggcggctc gacttgctcg
     2341 cctggtcggc cgaccgcgtc gttctgtttc gtaatacagg caaaggcttc tcggcgttcc
     2401 ctgtggaagg cgcgcgcggg ccggccttcg cgggcgattt caataacgac ggtctgcccg
     2461 atctttgtat cctcaacccg cccggcgcgg aactctggac caatcgcaaa ggatcgtttg
     2521 cgcggaccgc aaagctcccc ccggccggca gcttccgcaa ggctctgtgg gtcgatttcg
     2581 accacgacta cgatctcgat cttctgctga tgggcgaaca acaagcgctc ttgcgcaaca
     2641 acggcgacgg gtcttggtcc gatgtatctg gccagtttcc ctttgccgcc ggtcaggcct
     2701 tggacgccgt tgcgctcgag ctcgaaccga atggcaacac gttcgacatc gttgccgcgt
     2761 accgcaacag accggccgtg ctgtatcgcg accgcaaggc aggccgctac gaggccactc
     2821 ctctggcgtt tccgctggag ggcgcgctcg aaaccggcga cttcaatcag gatggctctc
     2881 tggatgttgc cgctgtcggc tcggcccgct tggcattcat cgagaaccgg ggcggaaacc
     2941 tggtgaggcg cccggatgtg ccgctggcgg cgggagcggg cgcgctgttt gcggattttc
     3001 agaatcgcgg ccgggtcgac gtcctggggg accgccggat gctgctctcc aacggagagt
     3061 tcacgtatca gaacggaaat gtgcgcggca cgaccggcgg atggctggcc gcggtggccg
     3121 gcgatttcaa caacgatggg ttaaccgatg cagccgtaat ccgcgacgga ggctcggttc
     3181 acctgttgct gaatctgacg ccgaaaaaga acaactggct taaggtcgcc ctgaccggcg
     3241 tgaagaacct gaagcttgcc tcgggcgccc gagtcgaggt caaggccggc ggcctttacg
     3301 aaaagcaggt ttaccagggc gtgccgctgg tcttcggttt agggggttac accttcgccg
     3361 acaccgtgcg gatcacctgg ccgaacggct tggtgcagaa cgagattcgg cagaaagctg
     3421 gcatgagtta taccttcaaa gaagctcccc ggctctcggg ctcctgtccg atgatcttca
     3481 cctggaatgg ccgccggttt gagtttatat ccgacgtcct gggggtggct cctatgggag
     3541 cgggcctggc ggaaggcgaa gtgtttccgc tcgatcacga cgaatacgtg cagattcccg
     3601 gccgcgccct tcagccgcga ggcggcctgt acgacgttcg cgtgaccgag gaactgcacg
     3661 aggtgtctta tctcgatcaa atccagctgg tggccgtgga ccatcctgcg ggaatcgaaa
     3721 tctttaccaa tgagaagttc aaatcgccgc cctttcccga cttccggctt tttggcgtga
     3781 agcgccgcat gtatccgagt gccgcccggg acgatcacgg ccgggatgtt cgtgagctga
     3841 tcttacgccg tgacgcccgc tacccggatg gcttccgccg cgatctgaac gggcgggccg
     3901 aaatgcactc cctgacgctc gatttcccga atctgccggc gcatcccgtt ttgttcttgc
     3961 acggctgggt ggactgggcc gacggcagta cgtttgtcgc cgcgtcgcag agccgcgccg
     4021 ggagcctggt gatcccctat ctccagg
//
LOCUS       DASREK010000177         8749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_254059, whole genome shotgun sequence.
ACCESSION   DASREK010000177 DASREK010000000
VERSION     DASREK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254059"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFA54_09140"
     CDS             <1..725
                     /locus_tag="VFA54_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZS51011.1"
                     /translation="TAADAGAQSWKTHLLSGRIDPGVAVTAGVLLGLWIRNSWQDCEL
                     ETIYGDQTAFYQLRIDPYYRTAAQRHPEAAGRVEELIAESSRRRVCLVHGDWSPKNLI
                     VGAGGIMVIDFEVTHFGDPAFDVAFCINHFLLKCFRCPDRASQLLDLSRTFYTWAAGV
                     VPAPALEFLEAAAARHLGWLLLARVDGKSPVEYIREEPLRDAVRRAALGIILEGPTDL
                     ETCYAIVKTELCRLEEAPAKTQ"
     gene            722..2029
                     /gene="eno"
                     /locus_tag="VFA54_09145"
     CDS             722..2029
                     /gene="eno"
                     /locus_tag="VFA54_09145"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZS51012.1"
                     /translation="MNFRIRKLAARQVLDSRGDPTVEVAVVLESGVSASASVPSGAST
                     SRHEARELRDGPGGGYQGRSVLRAVRNVNTELAAALRGWDVRDQSAIDRCMIHLDGTA
                     DKSRLGANAILGVSMAAARAAAASAGVPLWRHLAEIGTGGRRAMLPVPMINMISGGLH
                     AGRNIEFQDFLIVPHRFPSFAAALEAAVRIHRCIGVLLRAEGRASGGVADEGGFGPHL
                     ESNSQALDFVMRGIAAAGFRPGEEVSIAVDAAASHFYDAGQYRLPCENRSLTAEAMAA
                     MLEEWVGRYPIVSIEDPLAEDDWSGWKQITSALGRSCQLIGDDLFATNLGRLERGIRE
                     GVANAVLVKMNQIGTLSETLQVIDRARTAGYAAVISARSGETEDSFLADLAVASGAGQ
                     IKIGSVTRSERLSKYNRLLEIEERESTSPEPGRAPDPFPGRIV"
     gene            complement(1974..2411)
                     /locus_tag="VFA54_09150"
     CDS             complement(1974..2411)
                     /locus_tag="VFA54_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51013.1"
                     /translation="MSKKKIPENPAAADKNGWQEDARMNQEKLFRLSCRAARGLIEGL
                     ESGDLERIQRNLAAIHHYVDLAETVPAKSLHGAALYRREQLELLGGVTEDLAGALGKI
                     QKSLAARMEALGAHRRLLQHIVGAPAIQYDPETDPELALAPAK"
     gene            2504..2740
                     /locus_tag="VFA54_09155"
     CDS             2504..2740
                     /locus_tag="VFA54_09155"
                     /inference="COORDINATES: protein motif:HMM:NF018043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HZS51014.1"
                     /translation="MIRLTRLNHQPIVVNAELIEYLETTPDTVISLVNGQKMAVLETL
                     DQVMAEIINYQREIRQRPIVRAKEREQNREAESG"
     gene            2721..3554
                     /locus_tag="VFA54_09160"
     CDS             2721..3554
                     /locus_tag="VFA54_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HZS51015.1"
                     /translation="MKQSLDKGTLLGVCLALGGICGGLLLEGGSLREIAQPTAAMIVL
                     GGTIGATMVGQPFRVFLAACRELIRVLFETHTDPQAAIRQIIDFATKARKNGIVSLEK
                     EALAAPDPFLRKALTLAVDGTEPQEIRRMMELEIMLVEDRRAAESKVLEAAGGYAPTI
                     GIIGAVMGLIQVMKHLENIEEVGRGIAVAFVATIYGVGLANLLLLPAAAKIKAKAQSH
                     MKIAQLTLEGVIGIAEGLNPKLIQGKLEAYLDEKPTAVPAKNAAPKVAAAAAAEPAAR
                     S"
     gene            3557..4351
                     /locus_tag="VFA54_09165"
     CDS             3557..4351
                     /locus_tag="VFA54_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HZS51016.1"
                     /translation="MNKAHLHDHASHERWLVSYADFITLLFAFFVVMFASSQVNKKKV
                     AHIAAAFESYIKDGRVREEGIQAGAGPRDGSQSNGAAPEPSPEDISRRELDPVKSRLE
                     QQLKTEIGEGKLSISLEPRGLVLSLRESVLFPPGSDQFSAEAVPFLTKIAKSLQALPG
                     RPIRLEGHTDNIPIHTSQFPSNWELSAARATAVLELLTRKLEIKPERFAVTGYADYHP
                     LADNETAEGRARNRRVDIVILSRLAARMEPRPKPVDSQKPRDKPEE"
     gene            4467..5876
                     /locus_tag="VFA54_09170"
     CDS             4467..5876
                     /locus_tag="VFA54_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS51017.1"
                     /translation="MNTSPRVLVANPDERACSQTASSLGALGLAIDCASSGYACLEML
                     EGRRYQAVVSERHLGDLEAEQLLEQSRRRCPDLAFVVVSAEGGPQEAVRLTKKGLDDY
                     LPAGLPPERMAARLRELIGSRESAARASSGEPRGRSSDSAESVLIGRSPAIREVIETI
                     RLIAPKRSTVLITGPTGTGKELAAKLIHALSPRAAMPMVTVNCGAIPEHLLESEFFGH
                     VKGAFTGAVSARVGSFEQAHGSTLFLDEIGEMPFDLQAKMLRALQEREFQRVGSSHAV
                     QVDVRVIAATNCDLAAKVRRGEFREDLYYRLNVVPVPMPPLAARLEDVPLLIDHILAK
                     ICRRESIPLKRVGPGALELLSRYHWPGNVRQLENALEKAIILSGGAEELSSRDFDLPA
                     HSGFAGCSSEGDPADSAIQLPPGGMDLDAFLSKLERNLLEQALQRAAGNKKRAADMLG
                     LKRTTLSAKLRVAGIRFPA"
     gene            6033..6395
                     /locus_tag="VFA54_09175"
     CDS             6033..6395
                     /locus_tag="VFA54_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HZS51018.1"
                     /translation="MAALWGPSLLHLERCLDLLAARQRVVASNIANADTPGYRAREID
                     FRSELETFAAQSPPASFYPALLVREKWGQGAKNDGNNVDLDHELESLSENALRFSMAS
                     SMLQKQIQELRSAIREGK"
     gene            6414..6854
                     /gene="flgC"
                     /locus_tag="VFA54_09180"
     CDS             6414..6854
                     /gene="flgC"
                     /locus_tag="VFA54_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HZS51019.1"
                     /translation="MSLFSALQVSASGMAAQRTRVELLVENIANSETTRTPEGGPYRR
                     RDAVFTSEPEMAPFRALLAGNLSEPLSGVRVAEVVVDDSEPEVRYAPGHPDADANGYV
                     AFPRINPVEDMVDLTAAVRSYQANLAAMGAVKDMMRRSMDLLKA"
     gene            6957..7265
                     /gene="fliE"
                     /locus_tag="VFA54_09185"
     CDS             6957..7265
                     /gene="fliE"
                     /locus_tag="VFA54_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZS51020.1"
                     /translation="MNGLPPVNPLGSGFPDLGRIAGAAGTDAPKGSFAGALKDAIQRV
                     NDLQLQSDQELQDFLTGEGNDVHKSVLAAQRADLAFQMLLAVRNKVIDAYQEIMRIQV
                     "
     gene            7387..>8749
                     /gene="fliF"
                     /locus_tag="VFA54_09190"
     CDS             7387..>8749
                     /gene="fliF"
                     /locus_tag="VFA54_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZS51021.1"
                     /translation="MSKLIQQLQALWGSFTARQKASVVLAAVLVGGGLWAFERWSIER
                     NFRPLYTGMASEEAGAVIARLREMGVEYRLNDTGSAVLIPSDRVAETRLQLATEGLPR
                     HGRIGFELFDQTKFGTTDFAEQVNYRRALEGELERSVLSLSQVEQARVHISLAKQSVF
                     LDNQQPAKASVVVKLRRNQRLDDGQVRGVAYLVASAVEGLAPEMVSVLDVRGNLLSRP
                     RKPEREATEEEMELQQKIERETAEKILHTLEPYLGPNRARASVSADIDMNSGEQTEEI
                     LDPNPVALASQKSEELSQPAFAGGQPGTGSNVPRGAARPIFTSSSLSRKQENTTYQAG
                     RTVTHMRLEKGSVKRLSVAVVVDNKIATDAAGRRASVPRTPEEMKTIRDLVLASAGIL
                     ESRGDLLTIENLPFESQPSAEIPEGAPAAPVAAPGVERRFDIKEWLTRNRYRVIGAAV
                     ALAA"
BASE COUNT         1693 a         2633 c         2796 g         1627 t
ORIGIN      
        1 tgacggcggc ggatgccggc gcccaaagct ggaaaacaca tttgctctcg ggccgcatcg
       61 acccgggcgt ggccgttacc gcgggagtct tgctgggcct gtggatccgc aatagctggc
      121 aggattgcga actcgaaaca atctacggcg atcagaccgc tttctaccag ttgcggatcg
      181 acccctacta tcggaccgcg gcccagcggc atcccgaggc ggctgggcgg gtcgaagaat
      241 tgatcgcgga atcctcgcgg cgccgggtgt gcctggtgca cggagactgg agcccgaaaa
      301 atctgatcgt cggcgcaggg ggaattatgg tgatcgactt tgaagtgacc cactttggcg
      361 atccggcgtt cgacgtggct ttttgcatca atcatttcct gttgaaatgt ttccgttgcc
      421 cggaccgcgc atcgcaactg ctggatctgt cgcggacctt ttacacgtgg gcggcggggg
      481 ttgttccggc gccggcgctg gaatttttgg aagccgctgc ggcccggcat ctcggctggc
      541 tgctgctggc ccgcgtggac ggaaaatcgc cggtagagta catccgtgag gagccactac
      601 gcgacgccgt gcgccgcgcg gcgctgggaa tcattctcga ggggccgacg gatctcgaga
      661 cgtgctacgc gatcgtgaaa acggaactgt gccgactgga ggaggccccg gcaaagacgc
      721 aatgaatttt cggattcgga agcttgcggc gcggcaggtg ctcgactcgc ggggcgatcc
      781 aaccgtcgag gtggcggttg tgctcgagag cggcgtctct gcatccgcca gcgtcccttc
      841 cggagcttcg accagccgcc atgaagcccg tgaactgcgt gacggccccg gcggcggcta
      901 ccagggacga agcgtgctcc gcgccgtgcg caatgtgaat acggagctcg ccgctgcctt
      961 gcgcggctgg gatgtgcgcg accagtccgc tatcgaccgc tgcatgatcc atctggatgg
     1021 caccgcggat aagagccggc tcggcgcgaa tgccatcctg ggggtctcga tggcggcggc
     1081 tcgcgccgcg gccgcatccg ccggcgtgcc gctgtggcgc catctggccg aaatcgggac
     1141 gggaggccgc cgcgcgatgt tgccggtccc tatgatcaat atgatctccg gcgggctgca
     1201 cgccgggcgc aacatcgagt ttcaggattt cctgatcgtt ccccatcgct ttccctcctt
     1261 tgcggccgct ttagaagccg ctgtgcgcat tcaccgctgc atcggcgtcc tcttgcgggc
     1321 agaaggccgc gcttccggcg gcgtggctga cgaaggaggc tttgggccgc acttggagtc
     1381 caacagccag gctctcgatt ttgtgatgcg tggcatcgcg gcggcggggt ttcggcccgg
     1441 tgaggaggtt tcgatcgctg tcgatgctgc cgccagccat ttttatgatg ccgggcaata
     1501 ccggttgccc tgcgagaacc ggagtttgac ggctgaggca atggccgcga tgctggagga
     1561 atgggtgggc cgctacccga tcgtttccat cgaagatccc ctggcggaag acgactggtc
     1621 cggctggaag cagatcacca gcgcgctggg aaggtcctgc cagttgattg gagacgactt
     1681 gttcgccaca aacctcgggc gccttgaacg aggcattcgc gaaggcgtgg ccaatgcggt
     1741 gctagtaaaa atgaatcaga tcggcactct atccgaaacg cttcaggtaa tagaccgcgc
     1801 cagaacggca ggatacgcgg ccgtgatttc cgctcgttcg ggggaaaccg aggattcgtt
     1861 cctggccgac ctggccgtag cctcgggcgc tggccagatt aaaatcggct ccgttacccg
     1921 ttccgagagg ctttcgaaat acaaccggct cctggaaatc gaggagcgcg aatctacttc
     1981 gccggagcca gggcgagctc cggatccgtt tccgggtcgt attgtatagc gggcgcgccg
     2041 acgatgtgtt gcaagagcct gcggtgcgcg cccagggcct ccatcctcgc cgcgagactt
     2101 ttctgtattt tgcccagagc tccagcgaga tcttcggtga cgccgcccag cagctccagc
     2161 tgctcgcggc ggtaaagcgc ggcgccgtgc agcgacttgg ccggaaccgt ttcggccaga
     2221 tcgacgtaat ggtgtatggc tgccagattt cgttgaatcc gctccagatc tccgctctcc
     2281 aagccctcga tgagccctcg cgccgcccgg caagataatc ggaagagctt ttcctgattc
     2341 atccgcgcat cctcctgcca gccgttctta tcggccgctg cggggttctc gggaattttt
     2401 ttttttgaca aagaattccg cttacgcagg cagcccgtaa gcggtcctcg gtcgctgtta
     2461 accccagaag ctttggccga taggaaatag agatatttgg gtcatgattc gccttacgcg
     2521 gctaaaccat cagccgatcg tggtcaacgc cgagctgatt gagtacctcg aaaccacacc
     2581 ggacacagtg atctcgctgg tcaacggcca gaagatggct gtgctcgaaa cgcttgatca
     2641 ggtcatggcc gaaatcatca actaccagcg ggaaatccgg cagagaccga tcgtccgggc
     2701 aaaggagcga gaacagaatc gtgaagcaga gtctggataa gggcacattg ctgggcgtct
     2761 gcctggcctt aggcggtatt tgcggcggcc tgctgctcga gggcggcagc ctgcgggaaa
     2821 tcgctcagcc cacagcggcg atgatcgtgc tcggcgggac tattggcgcg actatggtcg
     2881 gccagccgtt ccgcgtgttt ctggccgcct gccgcgagct gattcgcgtt cttttcgaaa
     2941 cgcatacgga ccctcaggcc gccatccgcc agatcatcga cttcgcgacg aaggcgcgca
     3001 agaacggcat cgtctcgctc gaaaaggaag cgctcgccgc ccccgaccct tttttgcgaa
     3061 aggccttgac gctggccgtg gacggcacgg agccgcagga gatccggcgc atgatggaac
     3121 tggaaataat gctggtcgag gaccgccgcg ctgcggaatc caaggtgctg gaggccgcgg
     3181 gcggctacgc cccgaccatc ggtatcattg gcgcggtaat gggtctgatc caggtgatga
     3241 agcacctgga gaatatcgaa gaagtagggc ggggaatcgc agtggcgttt gtggcgacga
     3301 tttatggcgt aggtctcgcc aatctcctgc tgctgccggc ggcggccaaa atcaaagcca
     3361 aggcgcagtc gcatatgaaa atcgcccaac tcacgctgga aggcgtgatc ggcatcgccg
     3421 agggcctgaa cccgaagctg atccagggca agctcgaagc ctatctcgac gagaagccga
     3481 cggcggttcc cgccaaaaac gccgctccga aagtcgccgc tgcggctgcc gccgagccgg
     3541 ccgcccgatc ataaatatga ataaggctca cttgcatgac cacgccagcc acgagcgctg
     3601 gctggtctcc tacgccgatt tcatcaccct gttgttcgcc ttttttgtgg tgatgttcgc
     3661 ctcttcccag gtgaataaaa agaaggtggc ccacatagcc gcggccttcg agtcatacat
     3721 caaagacggg cgcgtccggg aggagggaat ccaggccggc gccggacccc gggatggctc
     3781 tcagagcaac ggcgctgctc cggagccttc gcccgaagac atttcccggc gggagctcga
     3841 tcccgtcaag tctcgtctcg agcagcagtt gaaaacggaa atcggggagg gcaagctctc
     3901 tatatcactg gaaccgcgcg gcctggtttt gagcctccgg gaatccgtgt tgttcccgcc
     3961 gggcagtgac cagttcagcg ccgaggccgt tccgtttttg accaagatcg ccaagtcgct
     4021 gcaagctctt ccgggccggc ccatccgatt ggaaggccat accgacaaca tccccatcca
     4081 tacgtcccag ttcccgtcaa actgggaact gtcggccgcc cgggccactg ccgttctcga
     4141 gctgcttacg cgcaaacttg agatcaagcc ggaacgcttc gccgtcaccg gctatgcgga
     4201 ctatcatccc ctggccgata atgagaccgc cgaaggacgc gcgcgcaacc gccgcgtgga
     4261 tatcgtgata ctcagccgcc tggcagcgcg aatggagccg cggccaaaac cggtggacag
     4321 ccaaaagccg cgcgataagc cagaggaatg acacacgctg acggtttctc gacagctcac
     4381 ggcgcaaatc tccgccaatt cgttaggaat ttcacggttc tgtctctggc tgccgaattg
     4441 ccttccaccc caagcccaaa aatggaatga acacatcccc gcgggtcctc gtggcgaatc
     4501 cggatgagcg ggcgtgcagc caaacggcga gcagcctggg tgcgctcggt ctggcgattg
     4561 actgcgcctc gagcggctat gcgtgtctgg aaatgctcga aggacgccgc taccaggccg
     4621 tagtgagcga gcgccatctc ggagatctgg aagccgagca attactcgag cagtcgcggc
     4681 ggcgttgccc tgatctggcg ttcgtcgtgg tttccgccga aggcggcccg caagaagccg
     4741 tgcggctgac aaaaaagggg ctggacgact atctgcccgc cgggctgccc ccggagcgca
     4801 tggcggcgag gctgcgggaa ctcatcggct cgcgtgaatc ggccgcccgc gccagctccg
     4861 gtgagccgcg cggccgctcg tcagactccg ctgagtcggt tttgatcggg cgaagcccgg
     4921 ctatccggga agtaatcgag acgatccggt tgattgctcc caagcgctcg acggtcctga
     4981 ttacgggacc gaccggcacg ggcaaagagc tggcagcgaa gctgattcac gcgctcagtc
     5041 cccgggctgc gatgcccatg gtcaccgtaa actgcggcgc aattcccgaa cacctgctcg
     5101 aatccgagtt tttcggccac gtcaaaggcg ctttcacagg agccgtttcc gcacgcgtag
     5161 gatcctttga gcaggcccac ggctccacgc tgtttctgga cgaaattgga gagatgccct
     5221 tcgatctcca ggccaaaatg ctgcgagcgc tccaggagcg cgagtttcaa cgtgtgggca
     5281 gttcgcacgc ggtccaggtg gacgtgcggg taatcgccgc caccaattgc gatctggcgg
     5341 ccaaggtgcg gcggggcgag tttcgcgaag acctttatta caggctgaac gtggttcccg
     5401 tgccgatgcc gcccctggca gcacgcctgg aagacgtccc gctgctgatt gaccacattc
     5461 tggcgaagat ctgccgccgg gagagtatcc ccttgaagcg ggtgggaccc ggcgccctgg
     5521 aacttctttc gcgctatcac tggccgggta atgtgcggca actggagaac gctttggaaa
     5581 aggctatcat tctgagcggc ggagcggagg aactgagcag ccgcgatttc gacctgccgg
     5641 cccactccgg cttcgccggg tgttcctcag agggcgatcc ggcggattcc gccatacagt
     5701 tgccgccggg cggaatggac cttgacgcct tcctgagcaa gcttgagaga aatctgctgg
     5761 agcaggccct gcagcgcgcg gccgggaaca agaagcgagc cgccgacatg cttggcctga
     5821 agcgcacgac attgtccgca aaacttcgcg tagccgggat tcgctttccg gcgtgacagg
     5881 ctgccgcgcc ctgccgtctc gttcgagctt cctgccgcgg tcccaccgcc aaaaaattgc
     5941 cgctgcggca aacatcgagc cgccgcgacg cagatctgtc ttgtattttc atcacgtgcg
     6001 gggtggaatg gattttgcaa ccggaggcgg cgatggcagc tctctgggga ccgtcactgc
     6061 tccatctcga gcgatgtctc gacctgctgg ccgcccgcca gcgggttgtg gcgagcaaca
     6121 tcgcgaacgc ggacacgccc ggctaccgcg ctcgcgaaat cgattttcgc tcggaactcg
     6181 agacctttgc cgcgcagtct ccgcccgcca gcttttaccc ggcgctgctg gtacgggaga
     6241 aatgggggca aggcgccaag aatgacggca acaatgtcga tctcgaccac gagctggagt
     6301 cgctttcgga aaacgctctg cgcttcagca tggcctcctc gatgctgcaa aagcagatac
     6361 aagagctgcg aagcgcaatt cgagaaggga aataaaaccg ggagcagacc tgtatgagtc
     6421 ttttttccgc gctgcaagtg agcgcctcgg gcatggcggc ccagaggacg cgggtggagt
     6481 tgctggtcga aaatatcgcc aactccgaga ccacccggac cccggaagga ggtccttatc
     6541 ggaggcggga cgcagtattc accagtgaac cggagatggc ccctttccgc gcgctcttgg
     6601 ccggcaatct gagcgagccg ctgagcggcg tgcgcgtcgc cgaagttgtg gtcgacgatt
     6661 ccgagccgga agtgcggtat gcgcccgggc acccagacgc cgatgccaac ggctatgtcg
     6721 cctttccccg catcaaccct gtcgaagaca tggttgattt aacggccgcg gtgcgatcgt
     6781 accaggccaa tctggcggca atgggggcgg taaaagacat gatgcgccgc tcgatggatc
     6841 tgctcaaggc gtagaacgct tgtcgagcga aacggcgagg ctaagctcca gttgtattga
     6901 aaaacagggg tacggttcag gccggacgcc ggtaggcctc aggagaaggt aagtaaatga
     6961 atggattgcc gcccgtcaac ccgctcggca gcggatttcc tgatctcggc agaattgccg
     7021 gcgcggcggg aaccgatgcg ccgaaaggga gcttcgcagg cgctctgaag gacgccatac
     7081 agcgcgtcaa cgatctgcaa ttgcagtcag accaggaatt gcaggatttt ctgacaggcg
     7141 aaggtaacga tgtgcacaaa tcggtgctcg ccgcccagcg cgccgatctg gcgttccaga
     7201 tgctgctggc tgtgcgaaac aaggtgatcg atgcctatca ggaaatcatg agaattcagg
     7261 tgtgagcagc cggaagatgg cagaggcctg agggcagcca ggaagcggga gactcccccc
     7321 gcccgcctgg atctcacctc gccggtcgtt cgcggacgtt tgttcctgag ccggcttttt
     7381 tcgtttgtgt ccaaattgat ccagcaattg caggcgctct ggggaagctt caccgcgcgc
     7441 cagaaggcca gtgttgtttt ggccgccgtg ttggtcggcg gcggtctgtg ggctttcgag
     7501 cgctggagca tcgagcgcaa cttccggccg ctctataccg gcatggccag tgaagaggcc
     7561 ggagcggtca tcgcgcgcct gcgcgagatg ggtgtcgagt accgtcttaa cgataccgga
     7621 agtgcggtcc tgatccccag tgaccgcgtc gccgagactc gccttcagct tgccaccgaa
     7681 ggtttgcccc gccacgggcg catcggcttt gaactttttg accaaacgaa gttcgggacc
     7741 accgacttcg ccgagcaggt caattaccgg cgggcgctgg agggggaact ggaacgttcc
     7801 gttctctcgt tgagccaggt ggagcaggct cgcgttcaca tcagcctggc caagcagtcg
     7861 gtgtttctcg ataaccagca gccggccaaa gccagcgtcg tggtaaaact gcgccgcaat
     7921 cagcggctgg acgacgggca ggttcgaggc gtggcctatc tggtggccag cgctgtcgaa
     7981 ggactcgcgc ctgagatggt ttccgtgctc gacgtgcgcg gcaatctgct ttcgcggccg
     8041 cgcaagcccg agagggaagc aaccgaagaa gaaatggagc tgcaacagaa gatcgagcgg
     8101 gaaacggccg agaagatcct gcacaccctc gagccgtatc tgggacccaa cagagcccgg
     8161 gccagcgtct ccgccgatat cgacatgaat tccggggagc agaccgagga aatcctggat
     8221 ccgaacccgg tcgcccttgc cagtcagaag tcggaggaat tgtcgcaacc ggcgttcgcc
     8281 ggggggcagc ctggaacggg ttcaaatgtg ccgcggggcg ctgcccggcc catcttcacc
     8341 agctcatcct taagcagaaa acaggaaaac accacctatc aagctggccg caccgtcacg
     8401 cacatgcggc tggagaaggg atccgtgaag cggctttccg tggctgtcgt cgtggataac
     8461 aaaatcgcaa ccgatgccgc gggccggcgc gccagcgtgc cgcggacgcc ggaggagatg
     8521 aagacgatcc gcgacttggt gctggcctca gcgggaattc tcgaatcccg tggagacctg
     8581 ctgaccatcg agaatttgcc gtttgaatct cagccgtccg cagaaattcc cgaaggcgcg
     8641 cccgcggcgc cggtcgccgc gcccggggtg gaacgccggt ttgacattaa agaatggctc
     8701 acccggaacc gatatagagt gataggtgct gctgtggctc tggcggctc
//
LOCUS       DASREK010000178        10709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_254618, whole genome shotgun sequence.
ACCESSION   DASREK010000178 DASREK010000000
VERSION     DASREK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254618"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..1375)
                     /locus_tag="VFA54_09195"
     CDS             complement(416..1375)
                     /locus_tag="VFA54_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZS51022.1"
                     /translation="MRVGVFTALLSKLSLDQVIEKLKSLGISTVELGTGNYPGGPHCS
                     LDMLKNKTALKEFKAKLADNGISISALSCHGNPLHPNKAIAKANDEVTRKTILLAEKL
                     GVPVVIDFSGCPGDSDSSKYPNWVTCPWPPDYLDVLNWQWEKKVAPLWTQRGKFASDH
                     GVKIAIEMHPGFVVYNPETLLKLRKIAGPSVGCNYDPSHMFWQGIDPIKAIRVLDGAI
                     FHVHAKDTQIYESNLPKTGVLDVKPYKDEANRSWIFRTVGYGHGAEWWNEFVSTLRMY
                     GYDYVLSIEHEDSLMSVEEGLTKAIQFLDKVVLKEKPAEMWWA"
     gene            1586..2773
                     /locus_tag="VFA54_09200"
     CDS             1586..2773
                     /locus_tag="VFA54_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZS51023.1"
                     /translation="MSLQDNEPFYRIAKLPPYVFAVINEMRAKARAAQLDVIDLGMGN
                     PDGATPRVVVNKLIEAARNPRNHRYSVSRGIPRLREEIAKRYKASYNVDLDPDAETIV
                     TMGAKDALAHLLFAVIGPGDAVVTPSPSYPIHQYGVLMAEGRGCWLPMPHPAAFLNSL
                     EDIYRRSSKPPQMVLISFPHNPTTQCVEREFFQAIIDLARRHGTMVVHDFAYADLGFD
                     GYRAPSILEIEGAKEVAVEIYTLSKSFNMPGWRVGFCVGNRKMIAALARIKSYLDYGV
                     FQPIQIAAIIALRECEDDTGNICATYQKRRDVLIAGLCRAGWPVEPPRGSMFVWAPIP
                     ERYCSIGSLEFAKLLLEKALVAVSPGIGFGPHGEGFVRFALVENESRIRQATRSIKQF
                     LRA"
     gene            complement(2975..3559)
                     /locus_tag="VFA54_09205"
     CDS             complement(2975..3559)
                     /locus_tag="VFA54_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51024.1"
                     /translation="MSSAIPCGSPGPRGDRLQVIMVVLDVGCGRKKQEPSAIGLDRQP
                     GSAADVLCELRHFPWPVRDDCAEKIYLSHFLEHQPDILRVMAEVHRIGKAGAEVTIVT
                     PHYSSPDSYTDPTHLFHLGFHSFDYFDRDSFPDFTYHSGGFRILERRLTFGGNAVLDN
                     VGRLLAFLSVSFYEKHFAWIFPARNLFCRLAVVK"
     gene            complement(3534..4370)
                     /locus_tag="VFA54_09210"
     CDS             complement(3534..4370)
                     /locus_tag="VFA54_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZS51025.1"
                     /translation="MRLVTYSAGGRTRAGMIGNGNVIDLGYGDIVSFLADGERARERA
                     MQLSESSDAATVLPLHAVQIHAPIPRPPKFLLIGLNYRDHAIESGMKIPDVPTVFAKY
                     SNAVIGPGEAIELPAISQQVDYEAELAFVIGKRGRNIPADRWREYVFGYTIVNDVSAR
                     DVQMATSQWTMGKTFDTFGPMGPELVSADEIADPHNLRISCEVNGRVLQDSSTSQLIF
                     RIPDLVAYISQVLTFEPGDVVSTGTPPGVGFARKPPVFLQAGDEVVVKVEGLGELRNP
                     VR"
     gene            4447..5205
                     /locus_tag="VFA54_09215"
     CDS             4447..5205
                     /locus_tag="VFA54_09215"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZS51026.1"
                     /translation="MLSLENKVAVVLGGASGIGKAIALGYAKAGADVVVSSRRKDMVE
                     ETAGELRKLGSKTLVRTSDVTDRTSLETFCQAVVDEFGRVDILAVTSGILIKCPTAEM
                     AEADWQRVIDTNLTGTFRANQIFGRQMIRQKQGVILNTGSMTSFVAFSEVAAYNASKS
                     GVRMLTETLAVEWAPHNIRVNAIAPGVFRTSLNARALDIPERLEAIIRRTPMGRIGSL
                     DELVGAAVFLVSDEAAFITGVTLPVDGGFLAKGI"
     gene            5351..5584
                     /locus_tag="VFA54_09220"
     CDS             5351..5584
                     /locus_tag="VFA54_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZS51027.1"
                     /translation="MKITVNNHGPLRIEGDFSILDASGKAFGLAGRTVISLCRCGHSE
                     NKPFCDGSHKRMGFQSEVQARDLPPPAPKPPLA"
     gene            5771..7009
                     /locus_tag="VFA54_09225"
     CDS             5771..7009
                     /locus_tag="VFA54_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HZS51028.1"
                     /translation="MKHPHYLVRPPTAILTRGFDPSLSVGSARPAVFRSSTYVFSSPE
                     AAERAFAIALGKVTAAPGEHIDLIYSRLSHPNAEILEDQILPLEPEARAAAVFNSGMA
                     AISTVFLTLVGPGASFVYTTPVYGGTLHLIHQVLEPFGMRGVPVPAGDSAALARAISG
                     ARDLRLVFIETPANPTLRMTDIPCAAAAARRRADRPVVAVDNTLLGPCFQHPLPLGAD
                     LVVYSGTKYLAGFSDMLAGVVLALDTDLISRLRGARALYGNILQPDECWLLDSRLPTV
                     TLRMNRQSKNAQHIVEKLAGHPKILRIHYPSLFDEPEQIAIRDRQARFPGAMFSLELK
                     GGKPAAYEFLRRLTIARNAVSLGGVETLICHPKTTTHSELSEEELAAAGIDDSLVRIS
                     VGVEDWRDLLRDFEEALQGV"
     gene            complement(7178..8659)
                     /locus_tag="VFA54_09230"
     CDS             complement(7178..8659)
                     /locus_tag="VFA54_09230"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZS51029.1"
                     /translation="MGAPGIVIIGAGPAGLTAAYELAKSGVTSTILEKDNVVGGIART
                     VNYKGYRFDIGGHRFFTKVTLVEQMWREVLGPDLIRRSRLSRIYYRGKFFYYPLRAFN
                     ALSNLGVWETVRCMASYGKARLFPRRPEPDFATWVTNRFGKRLFEIFFKTYTEKVWGI
                     PCSEIQAEWAAQRIKGLSLSSAIKNALFGELGKSKDQIIKTLIDEFDYPRHGPGMMWE
                     RTKEMIEAKGSRVIFNAPVERIYWREGRVEAVEAGGTVHPLAHLISSMPVRELIEKLT
                     PAPPEKLLQAAQNLHYRDFLTIALVVNGTNLFPDNWIYVHDRDVKLGRIQNFNNWSPE
                     MVPDTSKTCLGLEYFCFEGDGLWSASDGDLIELGKRELAALGLVNPADVIDGAVVRMP
                     KAYPVYDGGYEAAMDEVRRFLLRVPNLQLTGRNGMHRYNNQDHSMLTAILAARNILGG
                     RYDLWQVNVEQEYHEEGGEITIEEMQKLEATQPLVPQRRSDRP"
     gene            8867..9682
                     /gene="bla"
                     /locus_tag="VFA54_09235"
     CDS             8867..9682
                     /gene="bla"
                     /locus_tag="VFA54_09235"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HZS51030.1"
                     /translation="MRFRRFAILLLLAANGPAQEQDRPFPPHQVIGNVYFVGTASLGC
                     FLVATPQGNILINSGFEETVPVVRASIERLGFRFEDIKILLTSHAHDDHVGGHALVKK
                     QTGARVLVMEGDEETVETGFGGRWAACPVDRVLHDGDQVTLGGVTLVAHHTPGHTPGC
                     TSWSLAVPEGGRTYNVVIVGSLNVSSRLLGNTEYPEIVSDFLQSIGLLERLPCDVFLG
                     AHGSYYRLREKYAKLEKGGPNPFIDPEGYRRYIKEREEALLARLAEERKATRR"
     gene            10002..>10709
                     /gene="bla"
                     /locus_tag="VFA54_09240"
     CDS             10002..>10709
                     /gene="bla"
                     /locus_tag="VFA54_09240"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallo-beta-lactamase"
                     /protein_id="HZS51031.1"
                     /translation="MPCRILYSLSLVAAVSPLLLHAQPAGRMIPTEPLPPVLLPWDSE
                     GHTREVDNKPFPAHRVIGNLYYVGTADYASFLITSDQGHILINPDFEDSVPLIQKSIE
                     RLGFRFGDVKILLISHAHGDHCAGAARVKELTGAEVMVMDRDLDVMESGAKGSRGGFP
                     PVKVSRVLHDRDEVRLGGNTLVAYLTPGHTKGCTTWVTKIQENGKSYDVVIVGSANVN
                     DARQLAGNEAYPDMVEDY"
BASE COUNT         2092 a         3248 c         3228 g         2141 t
ORIGIN      
        1 ggacggcggc gcctccccgg aaaacgcgga cggagatggg ctgcggccgg aagccggcga
       61 tatttgctcc cgggcagcgg ctcccgtagc cggaccctcg gcgagcgccg ggcggggcgg
      121 ctgagaggcc gccgcgctcg caggaggagc agccgattcg ccgggcgcca cggcctccgc
      181 aagcggagtc gaatcaaaag ggcaaaactg aaagctgggt tcccacgttc tcccgcaggc
      241 cgggcatctc ttactcaaag gcgtggcatc gaacgggcag aactggaatt cgttgctgaa
      301 ctggcggccg cagcgggggc actgcctctg gtcttgcatg tttctgcccg gaacttttta
      361 ttctacatcc gcccatcccg gcgcagcacg cggccattta aggtcctgga tcggcctacg
      421 cccaccacat ctcggccggc ttttccttca gcacgacctt atccagaaac tggatcgcct
      481 tggtgagacc ctcttcgacc gacatcaggc tgtcctcatg ctcaatggag agcacataat
      541 cgtagccgta catacgcagc gtggagacaa actcgttcca ccattcggcg ccgtgcccat
      601 agcccactgt gcggaaaatc cagcttcggt ttgcttcgtc cttgtaaggc ttgacatcga
      661 gcacgcccgt tttaggcagg ttcgactcgt agatttgggt gtccttggcg tggacatgaa
      721 aaatggcgcc gtcgagcacc cggatcgctt tgatcgggtc gatgccttgc cagaacatat
      781 ggctcggatc gtaattgcag ccgacggacg gtccggcgat tttgcgcagc ttcagcaatg
      841 tctcggggtt gtagaccacg aagccggggt gcatctcgat ggcgattttt acgccgtgat
      901 cggaagcgaa ttttccccgc tgggtccaca gcggcgcaac ttttttctcc cactgccagt
      961 tcagcacatc cagataatcg ggcggccatg gacaagtgac ccaattgggg tatttggaag
     1021 agtcgctgtc ccccggacag ccggaaaagt cgatgaccac gggcaccccc agcttttcgg
     1081 ccagcaggat cgtcttgcgg gtcacctcgt cgttcgcttt ggcgatggcc ttgttcggat
     1141 gcagcggatt gccgtggcag ctcagggcgc tgattgaaat gccgttgtct gccagcttgg
     1201 ctttgaattc cttgagggcg gttttgtttt tcagcatatc caggctgcaa tgcgggccgc
     1261 cgggataatt acccgtcccc agttcgacgg tcgagatgcc gagggatttg agcttttcga
     1321 tcacctgatc gaggctcagc ttgctcagta acgcggtgaa cacgccgact ctcactcgat
     1381 ttctcctgtt tcagcgaaaa ttgtgcgcca gcggggtttc agaatagcac agacggcagg
     1441 ttccgggcgg gccggcggaa gctcccgcca gcgcgggatc ccggtggtga gatcgggcgt
     1501 ttctgcgggg gtgcagggtt gctttttgcg cggcgcaaag cggcaaactc ggcgtccagc
     1561 ggccgcagcg gttacaatga tgaagatgtc tctgcaagat aacgagccgt tctatcgaat
     1621 cgccaagctg cccccctacg tcttcgccgt catcaacgaa atgcgcgcca aggcccgcgc
     1681 cgcccagctc gacgtgatcg atctcgggat gggcaacccc gacggggcaa cgccccgtgt
     1741 ggtggtgaat aagctcatcg aggccgcgcg gaatccgcgg aaccaccgct attcggtttc
     1801 ccgcggcatc ccccggctgc gggaagagat cgcgaagcgc tacaaagcca gctacaacgt
     1861 ggatctcgat ccggacgccg aaaccatcgt caccatgggc gccaaagacg cgctcgccca
     1921 tctgctcttc gccgtgatcg gtccgggaga tgccgtggtg acgcccagcc cctcctatcc
     1981 catccatcag tacggcgtgt tgatggccga gggccgcggc tgctggctgc cgatgcctca
     2041 cccggcggcg tttctcaact cgctcgaaga catttaccgg cgctcttcaa agcctccgca
     2101 aatggttttg atctcttttc cgcacaaccc gactacgcag tgcgtggagc gcgagttttt
     2161 tcaggccatc atcgatctgg cccggcgcca cggcaccatg gtcgtgcacg acttcgctta
     2221 tgccgacctc ggttttgacg gctaccgcgc ccccagcatt ttggaaatcg agggggcgaa
     2281 agaagtggcg gtcgagattt atacactttc caaaagcttc aacatgccgg gctggcgggt
     2341 tggtttctgc gtgggaaatc gaaaaatgat agccgctctc gcccgcatta aaagctacct
     2401 cgattacggc gtgttccagc cgattcagat tgctgcgatc atcgctctgc gcgaatgcga
     2461 ggacgacacg ggcaacattt gcgcgacgta ccagaagcgc cgggacgtcc tgattgcggg
     2521 gctctgccgt gcgggctggc cggtcgaacc gccgcgcggt tcgatgttcg tgtgggctcc
     2581 gattccggaa cgctactgct cgatcggctc gctggaattc gccaagctgc tgctggagaa
     2641 agcgctggtc gccgtctcgc cgggcatcgg gttcgggcct cacggggagg gcttcgtacg
     2701 cttcgcgctg gtcgaaaacg agagccgcat ccgccaggcg acccgctcga ttaaacagtt
     2761 cctgagagcc tagcgcgtta caactcccgc agaaccgaac ctgcaactat tccccagacc
     2821 ggcgccgctg gacgatcact gggatccagc ggattcggcg gccgcaggtc caggggtcat
     2881 ctccgcggga cttcggagga catttatacg actacatata cgcagccgtc cccagccact
     2941 atctttctcg acgacgaaag gcctttcgat ctgcttactt caccacggcc agccggcaaa
     3001 acaaatttcg cgcgggaaag atccaggcga agtgtttttc ataaaagctc accgatagaa
     3061 aagcgagcaa acgtcccacg ttgtcgagca ccgcgttgcc gccgaacgtc agccgccgct
     3121 ccagtatccg gaaccccccc gaatggtacg taaaatccgg gaagctgtcc cggtcgaaat
     3181 aatcaaagct gtggaagccg agatggaaca ggtgcgtggg atcggtatag gaatcgggcg
     3241 agctgtaatg gggagtgacg attgtcacct ccgcgccggc cttcccgatg cggtgcactt
     3301 cggccatcac gcgcagaatg tcgggctggt gctcgagaaa atgcgagaga tagatctttt
     3361 ccgcacagtc gtcgcggaca ggccagggaa aatgccgcaa ttcgcacagg acgtcggccg
     3421 ccgagccggg ctggcggtcc aggccgatcg ccgaaggctc ctgcttcttc ctcccgcatc
     3481 cgacgtcaag aaccaccata atgacttgga gccggtcgcc gcggggtccc ggactaccgc
     3541 acgggattgc ggagctcacc cagtccttcg accttcacta cgacctcgtc gcccgcttgc
     3601 aggaaaaccg gcggcttgcg cgcgaagccc actccgggcg gcgtgccggt ggaaacaacg
     3661 tcgccaggct cgaaagtgag cacctgagag atataggcga ccaggtccgg aatgcgaaag
     3721 atcaactgag atgtcgaaga gtcttgcagc acccgcccgt tcacttcgca gctgattcgc
     3781 aggttgtgtg gatcggcgat ctcgtcggcg ctcaccagtt ccggtcccat ggggccgaat
     3841 gtatcgaagg tttttcccat ggtccactgc gaggtggcca tctgcacgtc gcgggcgctc
     3901 acgtcgttca cgatcgtgta gccgaaaaca tattcccgcc atcggtccgc cgggatgttt
     3961 cttccgcgct tgccgatcac gaaagcgagc tcagcctcgt aatcaacctg ttgggaaata
     4021 gcgggcaact cgatcgcctc gcccggccca atgacggcgt tggagtactt ggcgaaaacg
     4081 gtcggcacat ccggtatctt catgcccgat tcgatggcgt gatcccggta attcaagccg
     4141 atcagaagga atttgggagg ccggggaatg ggagcgtgaa tctggacggc gtgcagcgga
     4201 agcaccgtgg ctgcgtcaga gctttcggag agctgcattg cgcgctcgcg cgcccgctcg
     4261 ccgtcggcca gaaaagaaac gatgtcgccg tagcccaggt cgatcacgtt cccgttgcca
     4321 atcattccag cccgcgttct gccgccagcc gaataggtca ccaatcgcat aggcgatcat
     4381 catacaatag tcaggttgca ggcagcgcag agccgccaat cccagggagc aggaatccgc
     4441 ggaaagatgc tgagcttaga aaacaaagtg gcagttgttt tgggtggcgc cagcggcatc
     4501 ggcaaagcca ttgcactggg ctatgcaaag gccggagcag acgtcgtggt cagcagccgc
     4561 cgcaaggata tggtcgaaga aacagcgggc gagctgcgca agctcggctc gaaaacgctc
     4621 gtgcgcacct ccgacgtcac cgaccgtact tcgctggaga cattttgcca ggctgtggtg
     4681 gacgagttcg ggcgcgtgga cattctcgcc gtcacgtccg gcatcctcat caagtgcccc
     4741 acggccgaga tggcagaagc agactggcag cgcgtgatcg acacgaacct gaccgggacc
     4801 ttccgcgcca accagatttt tggccgccag atgattcggc aaaagcaggg cgtaatcctg
     4861 aataccggct cgatgaccag ttttgtggca ttctcggaag tggctgctta taacgccagc
     4921 aagtcggggg tgcgcatgct cacggagacg cttgccgtcg agtgggcgcc gcacaatatc
     4981 cgggtcaacg cgattgctcc cggggtgttt cgcacgtcct tgaacgcgcg ggccctcgat
     5041 atccccgaac gcctcgaagc catcatccgg cggactccca tggggcgtat cggctccctc
     5101 gatgaattgg taggcgccgc cgtgttcctg gtttccgacg aagccgcttt catcaccggc
     5161 gtgacgctgc ccgtggacgg tggcttcctg gccaaaggaa tctagccgca gaggcgtgtt
     5221 tcgcgggccg ccggatctga gcgccatgga gacacggcaa aatctggagc gtgggctttc
     5281 tccaggcaga aacgaaaaag gcaaataact ctcgctatag gtcgaatctc tgcaagactt
     5341 atcgaaattt atgaaaatca ccgtaaacaa tcatggaccg ctgcgtattg aaggggattt
     5401 cagcatcctg gatgcttccg ggaaagcctt cggtttggcc ggccgcaccg tgatctcgct
     5461 ctgccgttgc gggcactcgg aaaacaagcc gttttgcgac ggaagccaca agcgaatggg
     5521 cttccagtct gaagtccagg cgcgcgatct gcccccgccc gccccgaagc cgccgctggc
     5581 ttagactccg cgctgcggcc tgtcccgaat ccgcgttgtg ctgctgaacc tgccgctcgt
     5641 tggcggcggc cttcgggttt gtgtgcgcgg ccgctgttga gaaacgctct ccgtgaagcg
     5701 ctggaggcat cgcaggcaaa cggacaattt cgcgggaggg cgccgactag aatggagtga
     5761 ggaggttttc gtgaagcacc ctcactacct ggtcaggccg cctacagcca ttttgacgcg
     5821 cggcttcgac cctagcttgt cagttggctc agcccggccg gcggtgtttc gctcatcgac
     5881 atatgttttt tccagcccgg aagcggccga gcgcgctttt gcgatcgcgc tgggcaaggt
     5941 aaccgcggcg cccggggagc acatagacct gatctactcc cgcttgtctc atcccaatgc
     6001 cgagattctc gaagaccaaa tactcccgct cgagccggag gcccgcgccg ctgcggtctt
     6061 caactccggc atggcggcca tcagcaccgt tttcctgacc ctggttggtc ccggcgcttc
     6121 ttttgtgtat acaacgccgg tttacggcgg cactctccat ttaattcatc aggtgctcga
     6181 gccgttcgga atgcgcgggg tgcccgttcc ggccggcgat tctgcggcgc tggctcgcgc
     6241 catttcaggc gcccgcgatc ttcgcctggt cttcattgaa acacccgcca acccgaccct
     6301 gcgcatgacg gacattcctt gcgcggcggc cgccgccagg cgccgtgcgg accggccggt
     6361 ggtggctgtg gacaacacac tgctcggtcc ttgctttcag catccgctgc cgctcggagc
     6421 cgacctggtg gtctattcgg gaacgaagta cctggccggc ttcagcgata tgctggccgg
     6481 ggtcgtgctt gccctcgaca cagatctgat ctcgcgcctt cgcggcgccc gcgccttata
     6541 cggaaacatc ttgcagccgg atgagtgttg gctgctggac agccgcctgc ccaccgtcac
     6601 gctccgcatg aaccggcaaa gcaaaaacgc acagcacatc gtcgaaaagc tcgccggaca
     6661 tcccaagatc cttcgcatcc actatccgag cctattcgat gaaccggagc agatcgccat
     6721 tcgagaccgc caggcgcgat ttccaggcgc catgttttcg ctcgagctga aaggcggcaa
     6781 gcccgccgct tatgaatttc tccggcggct cacgatcgcc cgcaacgccg tcagcctggg
     6841 cggcgtggaa accttgatct gccacccgaa aacaaccacg cactccgagc tttcggaaga
     6901 agaactggcc gccgccggca ttgacgattc cctcgtgcgg atctcggtcg gcgtagagga
     6961 ctggcgggat ttgctgcgcg actttgagga ggcgcttcag ggcgtataga tcggagaatg
     7021 gatatcggca accggtgaat cgatcgccgt tccgccgtta aataatggaa ccgctggaag
     7081 cgccggcgcg cagtgtaagt tcagcggccg gcggcggggg atcggcggtc ccggacgccg
     7141 cagggggtgt tttcctcgga cgatcaggag acgttgttca cgggcgatcg gagcggcgct
     7201 gcgggacgag cggctgtgtg gcttcgagct tctgcatttc ctcgatcgtg atctcgccgc
     7261 cctcttcgtg atactcttgc tcgacgttca cctgccacag atcgtaacgg ccgcccagga
     7321 tattgcgcgc cgccaggatc gccgtcaaca tggagtggtc ctgattgttg tagcgatgca
     7381 tgccgttccg gccggtgagt tgcaggttgg gtacgcggag gagaaatcgt cgaacctcat
     7441 ccatcgcggc ttcgtagccg ccgtcataga ccggataggc cttgggcatg cgcactactg
     7501 cgccatcgat cacatcggcc gggtttacca ggcccagagc cgccagttcg cgcttgccca
     7561 actcaatcaa atcgccgtcg cttgcgctcc acagcccgtc gccctcaaag cagaaatatt
     7621 ccaggccgag gcacgttttc gaggtgtcgg gaaccatctc ggggctccaa ttgttgaagt
     7681 tttgaatgcg tccgagcttc acatcccggt catgcacgta gatccagttg tcgggaaaca
     7741 gattggttcc gtttaccacg agcgctatgg tgaggaaatc ccggtaatgc agattttgag
     7801 cggcttgcag cagtttttcg ggaggcgcgg gagtgagctt ttcgatcagc tcacgcaccg
     7861 gcatgctgct gatgaggtga gccagcggat gcacggtgcc gcccgcctcg acagcctcga
     7921 ctcttccctc gcgccagtaa atccgctcga cgggggcgtt gaagatgacc cggctcccct
     7981 tggcctcgat catctctttg gtccgctccc acatcatgcc gggtccatgg cggggataat
     8041 cgaactcatc gatcagggtt ttgatgattt gatccttgct cttgcccaat tcgccgaaca
     8101 gagcattctt gatggccgaa ctgagcgaaa ggcccttgat gcgctgagcg gcccactcgg
     8161 cctgaatctc gctgcatggg atgccccaaa ctttttccgt ataggtttta aagaaaattt
     8221 cgaacagccg ctttccgaag cggttggtaa cccaggtcgc gaaatcgggc tccggccgcc
     8281 gcggaaacag cctggccttt ccatagctgg ccatgcatcg cacggtctcc cacactccga
     8341 gattcgagag cgcgttgaag gcccgtagcg gataatagaa aaacttccct cgatagtaga
     8401 ttcgggacag gcgcgagcgc cggatcagat ctgggcccag gacctcgcgc cacatttgct
     8461 cgaccagcgt cactttcgtg aagaagcggt gaccgccgat atcgaagcga tagcctttgt
     8521 aattgacagt gcgggcgatg cccccgacga cgttgtcctt ttcgaggatc gtcgaagtca
     8581 caccggactt ggcgagctca taagcagcgg tcaagccggc cgggccggcg ccgatgatga
     8641 ctatgcccgg agctcccata tacaaaggtt catcatagca gccacaccgc tgccttccct
     8701 agggcgaata tcccaattgg ggccgccctg ttgtctgacg acggccggaa actcgcgccg
     8761 gtcgcgccct gttggctggt ccaacataca gcttcaggag atcttcctct cggctcagga
     8821 tgacaggatt cgtgccagcg cctacactgg gaaaggaaaa acgcaaatga gattccgccg
     8881 gttcgcgatt ctgctgctgc tggctgccaa tggtccggcc caagaacaag accggccgtt
     8941 tccgccgcat caggtgatcg ggaatgtcta tttcgtgggc accgcgagct tgggctgttt
     9001 tttggttgcc acgccgcagg gaaatattct gatcaacagc gggtttgaag aaaccgttcc
     9061 ggtggtgcgc gcgagcattg agcggctcgg cttccgcttc gaagacatta agatccttct
     9121 caccagtcat gctcatgatg accacgtagg cggccacgcc ctggtcaaga agcaaaccgg
     9181 cgcgcgagtg ctggtaatgg aaggcgacga agaaactgtc gagacggggt tcgggggacg
     9241 ttgggctgcc tgccctgtgg accgggtgct ccatgacggc gatcaagtga ctctcggcgg
     9301 cgtgacgctg gtagcgcacc acacgcccgg acacacgcca ggctgtacgt cgtggagcct
     9361 ggccgttccc gaaggcgggc gcacctacaa cgtggtgatc gtgggcagct tgaacgtcag
     9421 ttcgcggctg ctaggcaaca cggaatatcc ggaaattgtg agcgattttt tgcaatccat
     9481 cggccttttg gagcggctgc cgtgcgacgt ctttctcgga gcgcacggct cctactaccg
     9541 gcttcgggaa aaatacgcaa aactcgaaaa aggcggtcca aaccccttca ttgatcctga
     9601 gggctatcga cgatacatca aggagcgcga agaagccttg ctcgcaagat tggcggaaga
     9661 acgcaaggca acgagaaggt aagcggagct tgtccgcacg gcgcgaccct ccccggccag
     9721 gcccgaacaa ccggccggtg ctctgaccgg ttggcgaggg cgcgaagcgc tgcgctggtt
     9781 cggaaaacag gccgaaccgg cgctctccac ttcccagcag atcattttct gttcgctgag
     9841 acgggggaga gcggcgtcga ccccaatttc ggtcctggct gctggagccg ggccgttgtt
     9901 tttcccgacg agcctggcgc cgtcaggaag aaaaaatcgt aatatagcgt aatcgagcga
     9961 gcgtgcggcc gtgccctggc cgcagaaagg acacttgggt tatgccttgc cgcatcctct
    10021 actctctttc gctggtggct gctgtttcgc ccctgttgct tcatgcccag ccggccgggc
    10081 ggatgattcc gaccgagccg ctgcctcccg tgcttctgcc atgggattcc gaagggcata
    10141 cgcgggaggt ggataacaag ccgtttcccg cgcatagggt aattggaaac ctctactatg
    10201 tcggcaccgc ggactacgct tccttcctga tcacctccga tcagggacat attctcatca
    10261 acccggattt cgaagactcc gtcccgctga tccaaaagag tattgagcgg cttggctttc
    10321 gcttcggcga cgtgaaaatt cttctgatca gccatgcgca cggcgatcac tgcgctggcg
    10381 cggctcgagt taaagaactg accggcgccg aggtgatggt tatggaccgc gacctggacg
    10441 ttatggaatc cggcgctaag ggcagccgcg gaggctttcc gcctgtaaag gtcagccgcg
    10501 tcctccacga cagggacgaa gtccggctgg gcggcaatac gctggtcgcc tacctgacgc
    10561 ccggccacac caagggctgc accacttggg tgacgaaaat ccaggagaac ggcaaatctt
    10621 acgatgttgt gatcgtaggc agcgccaacg tgaacgacgc ccggcagctt gccggcaacg
    10681 aggcctatcc cgatatggtg gaggattac
//
LOCUS       DASREK010000179         9695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_583356, whole genome shotgun sequence.
ACCESSION   DASREK010000179 DASREK010000000
VERSION     DASREK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583356"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1201
                     /locus_tag="VFA54_09245"
     CDS             44..1201
                     /locus_tag="VFA54_09245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS51032.1"
                     /translation="MPAAGYFRLIAERYRRRRFHSLFLFVTSRCNSRCRTCFYFDKLN
                     SPDDLSFAEIKRLSATAPAFRKLWISGGEPSLREELGEIIELFVRNNGVENVNLPTNG
                     LLPEKLFRSVDYALDHCPAASIDLNFSLDGLANTHDAIRRVPNNFQKTLQTIEEAERR
                     YRGVRRLRRNVVTVITSENYRELLDLGRLILSLGYERAQGHYFEVVRGETPDGNLQAM
                     PLQQVRALHRRLMPVYREYADRLFGHLPWMIRQFAAVYYLGNIRLHFELHEQCLERPR
                     RWPMPCTAGETALVVDHNGAFRACELRGVVGRLQDYDFNLSAALASGAMRAEVAEIPR
                     ANCWCTHSCFIQDSSKFSPRVQLFHIPWMYVRQLMERGSAGEVEAFKALEL"
     gene            1250..1651
                     /locus_tag="VFA54_09250"
     CDS             1250..1651
                     /locus_tag="VFA54_09250"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HZS51033.1"
                     /translation="MIVVAAGVIERDGRVLICRRRPEQTHPLKWEFPGGKVESGEQPA
                     EALRRELREELGIEMGAASEITRFSFQYPGRDPILLVFYRVHGYSGHVENRIFSEIQW
                     ADPRRLPELDFLEGDVDFIRQFARGWQERGE"
     gene            1755..2468
                     /locus_tag="VFA54_09255"
     CDS             1755..2468
                     /locus_tag="VFA54_09255"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZS51034.1"
                     /translation="MLRYFGLSDVGCVRTSNEDAYAIEPELGLFVVADGMGGAQAGER
                     ASAVAIEAIVENIRRAFPDRSPDRLLDAVQAANRTVLQQASADPDLQGMGTTVVAVLV
                     APPAAHIASVGDSRAYLFRNGRLERITSDQTWVNEVGRSLGLTEEQIHTHPFRNVLTM
                     AVGARERIEVHRYELSLQPADLLLLSSDGLHGVVSEESIAEALGAKTSLAAKAADLIE
                     MARANGGPDNITAVLVENA"
     gene            2473..3186
                     /locus_tag="VFA54_09260"
     CDS             2473..3186
                     /locus_tag="VFA54_09260"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HZS51035.1"
                     /translation="MRHYAAQRIVLVLALLTGGDLLCFAQRGAKQSARDLFYSEAGLI
                     VSSQNKGRGRFGAAKQSTVAVALGLKYRIWTVSGEQAVEADPAGLFSPGDVVRLGVEV
                     NDAGYLYVVQWQPGGAWKRLFPTPEIEQGSQFVRSGIVYSIPAGGRRLDFSAGLERIL
                     ILFARQPIRELEPLIAAPGRAGSDPVVADAILEKARKGIDERALVTETAASEKCVYVV
                     NRTGKPDSALAAEIRLSSR"
     gene            3363..4259
                     /locus_tag="VFA54_09265"
     CDS             3363..4259
                     /locus_tag="VFA54_09265"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZS51036.1"
                     /translation="MPSYRYFQAAPPPPKAVDYRADLLAAIEDLPPLPLILNRLLQLL
                     NDSNASSAQIAAMIEKDAVLSGSVLRSVNSAYYGLKATVTSIRHAVSMLGFSTVRNLA
                     LAFSMRRMLTRSRTPPPRLYARYSQHSLSCAMLCHYLAGYTGLNDSEAGFAAGLFHDI
                     GKLLILTSFPDLLPKVIEHYEQSECSYEQAEIDILQVSHSELGRIVLQKWQLPECIQQ
                     AVEFHHHPADCPRESGAITLADIVHAADIYVVEYGLDILPTKRGHPHPADMAFAEIGL
                     RQKMPEVLEKFKNEYESIRSLF"
     gene            4497..4790
                     /locus_tag="VFA54_09270"
     CDS             4497..4790
                     /locus_tag="VFA54_09270"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HZS51037.1"
                     /translation="MNALLQFSYLQLLDVLTTLAFLANGVKEANPLVRFLLKAGPSPL
                     AALILVKGLAFALAIYCVRNSRQRLLSRVNMGFAALVMWNLCILVISGPQISG"
     gene            complement(4765..7740)
                     /locus_tag="VFA54_09275"
     CDS             complement(4765..7740)
                     /locus_tag="VFA54_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HZS51038.1"
                     /translation="MDRLLESVPFRHVERAQRSLAGLSRYLSSETENILNSLLAQSPD
                     PDAAIHYLDRFLSSGAEAAGTLARTSRLNALLAVFSYSHFLAEAIFQHPDWLDCVSEQ
                     NGFYRVLGPEELRSQLGPFPREMRREAVALELARFRRRNLLRIMLRDVLGLSTLAETT
                     LELSNLADTILEAALERIQAELTEIFGSPRLAPAGAAASPEKRAGGGQLCEFTVLALG
                     KLGGRELNYSSDIDLMYFFAGGGETDGPKRLTNKEFFIKVANQLTDLLSSNTPEGSCY
                     RVDLRLRPEGNLGEVAQPLSGLRDYYQRRARDWELQMLIKARVAAGSRELGRRFLRFV
                     EPLIYRTTTDFSAIESVSETRERIHEKLRRRAPGGLNVKLSRGGIRDIEFLVQCLQRL
                     YGGRDAWVRHGGTLVALQRLRDKRYISARDYARLNSAYHFLRIVEHRLQFDYDRQTHT
                     LPDEPEALEVLGFKVGVPNGEKIAGTLVERTEQHLRAVAEIYDRVIHGHSPVSLQGPD
                     AAPLFQLEMEAPQENLERGMQAQLRYVELRAPAVAARIAQAPLKRGRGLFEHFLGKIV
                     SMPDLLTAMEEQPELVDLTVDLFEHSPFLAEWLIRNPEEIHQLLEVAAFSASDQQAPL
                     FPLEAPRRNFPGDHPELEHLLRTHAPVNEKTAWLRRFYRRQMLRIQTESICRRSPIFA
                     TLEQTTELADWVVRAAYRIALDEVLHEAPSTLMHVVALGRLGMREFDLGSDADLVFIV
                     PDEAKPQLDRWTRVAERTIEVLTSYTGEGVIFSVDTRLRPLGREGELVQTEGQYSKYF
                     SGQAQAWEAITYMKSRCLAGDIERGTAFLHELQNVDWRRYGQSGSLAGLLSEMRRKLE
                     REQGAEHPLKAGPGGYFDIDFVLMYLRLRGAGIFYTSLNTPERIDVIERMGLLSRENA
                     EFLHTAAVFYRALDHALRVSTGRSLGMLPKSRSQYEIVTELVRRWTPQSLHGRPMDQL
                     LAEMRQRTRETFNQIFEAR"
     gene            complement(7818..9239)
                     /gene="glnA"
                     /locus_tag="VFA54_09280"
     CDS             complement(7818..9239)
                     /gene="glnA"
                     /locus_tag="VFA54_09280"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZS51039.1"
                     /translation="MSIVRSPKEVLEFAKANGAQMLDMRFSDLPGLWHHVSFPIAQLT
                     ESSFEDGFGIDGSSLRGWAAIHESDMLLIPDASTCMMDPFTQIPTLVMIGDVIDPITK
                     QHYERDPRWIAKKAENYLKYSGIGDIAYFGAEAEFFIFDNIRFDQTQNSGYYFIDAEE
                     GRWNSGRSENNLGYRPRYKEGYFPVPPTDHYQDLRAEMVRTMLKSGLEIECHHHEVAT
                     GGQAEIDQRFDSLVKSADNMMIYKYVIRNVAAQHGKTVTFMPKPLFQDNGSGMHTHQS
                     IWKNGTPLFAGDRYAGLSDIALWYIGGLLKHAPAVVALAAPTTNSFKRLVPGFEAPVN
                     LAYSRRNRSAACRIPMYSASPKAKRVEFRPPDPSCNPYLAFAAMLMAGLDGIQNKIDP
                     GEPLDKDIYDLSPEEMRGVPSLPASLDEALKALENDHAFLTKGDVFTEEIIETWIDYK
                     MKNEVDAIRLRPHPYEFAMYYDI"
BASE COUNT         1808 a         2913 c         2993 g         1981 t
ORIGIN      
        1 cggcggccag cgctgaaaat gaaataggat cccgccccac aagatgccgg ctgccggata
       61 ttttcgattg attgcggaac gctatcggcg ccgccgcttc cactctttgt ttctgttcgt
      121 cacctcgcgc tgcaattccc gttgccgcac ctgcttctac ttcgacaagt tgaacagccc
      181 ggatgatttg agcttcgccg aaatcaagcg tctttcggcg acggcgcccg ccttccggaa
      241 gctgtggatc tcgggcggcg agccctccct gagggaagag ttgggcgaaa tcatcgagct
      301 gttcgtccgc aacaacggcg tcgagaatgt gaacctgccg accaacggcc tgctccccga
      361 gaagctgttc cggagcgtcg attacgcgct tgaccactgc ccggcggcgt caatcgatct
      421 gaatttttcg ctggatggtc tggccaatac acacgatgcg atccgccggg tgccgaacaa
      481 ttttcaaaaa acattgcaga ccattgaaga ggccgagcgg cgctatcgcg gcgtccgccg
      541 gctgcgccgc aacgtggtca cggtcatcac cagcgaaaat taccgggagc tgctggatct
      601 gggccggctg attttgtctc tgggatatga gagggcgcaa ggccactact tcgaggtcgt
      661 gcggggagaa acgcccgacg ggaatctcca ggcgatgccg ctccagcagg tgcgagcctt
      721 gcaccgccgg ctcatgccgg tataccgcga gtatgcggac cggctgttcg gccatttgcc
      781 gtggatgatc cggcagtttg ccgccgttta ttacctggga aacatccggc tgcacttcga
      841 gctgcacgaa cagtgcctgg agcggccccg ccgctggccg atgccgtgca cggccggcga
      901 gaccgcgctg gtcgtcgatc ataacggcgc cttccgggct tgcgagctgc gcggcgtggt
      961 gggcaggctc caggattatg atttcaacct ctcggcggcg ctggcttccg gcgcgatgcg
     1021 cgcagaggtg gccgagattc cgcgggcgaa ctgctggtgt acgcatagct gctttatcca
     1081 ggacagctcc aagttttccc cgcgggtgca gttgtttcac atcccgtgga tgtacgtgcg
     1141 tcagttgatg gagcggggca gcgccggcga agtcgaggcc tttaaggcgc tggaactgta
     1201 gaaagccctt taatccggcg gaaaacgcag cgaggcctgt gcaatctcca tgatcgttgt
     1261 ggccgcaggg gtcatcgagc gcgatggccg ggtgctgata tgccggcggc gcccggagca
     1321 gacgcatccc ttaaagtggg aattcccggg cggaaaggta gagtccggcg agcaacccgc
     1381 cgaggctttg cgccgcgagt tgcgggaaga gctcggaatt gaaatgggag cggcgtccga
     1441 aatcacccgt ttttcgtttc agtatcccgg ccgcgatccg atcctgttgg tcttttatcg
     1501 agtccatggc tattccgggc acgtggaaaa ccgcattttt tccgaaattc aatgggccga
     1561 tccccggcgc ctcccggagc ttgattttct ggagggcgac gtggatttta tccgccaatt
     1621 tgcacgcggc tggcaggaac gcggggaata ggaatcctgg ctgcgttctt cttgatcttt
     1681 tcggttccgc cgggtctgtg ctgcaatcct ccagagtgaa cgcctgacct tcaaaatgct
     1741 aaactgacgg agccgtgctc aggtactttg gtctctccga tgtcggatgc gtaagaacct
     1801 ccaatgaaga tgcctacgcc atcgagccgg aactcggtct gttcgtcgtg gcggacggca
     1861 tgggaggagc ccaggcgggc gagcgggcct cggccgtggc tattgaggca atcgtcgaaa
     1921 acattcgccg ggcgtttccg gatcgtagcc cggaccgcct gctggatgcc gtccaggcgg
     1981 cgaatcgcac cgtcctgcaa caagcttcgg ccgaccccga cctgcaagga atgggcacga
     2041 cggtagtggc cgtgctggtc gcgcctccgg cggcgcacat cgcgagcgtg ggagacagcc
     2101 gtgcttacct gttccggaac ggccggctgg agcgcattac ttccgatcag acctgggtga
     2161 atgaggttgg ccggagcctc ggcctaaccg aagagcagat tcacactcat ccgttccgaa
     2221 acgtgctgac gatggccgtt ggcgcgcggg agcggatcga agtccatcgc tatgagctca
     2281 gtctccagcc ggcggacctg ctgctgcttt cctccgacgg tctgcacggg gtggtttccg
     2341 aggaaagcat cgcggaggcg ctcggcgcga aaacttcttt ggccgccaag gcggcggatt
     2401 taatcgagat ggcgcgcgcc aacggaggtc ccgataatat caccgctgtg ctggtcgaga
     2461 acgcctgagg gtatgaggca ttacgccgca caacgaatcg ttttggtctt ggcgctgctg
     2521 accggcgggg atctgttgtg cttcgcccag cgcggggcca aacagagcgc gcgcgacttg
     2581 ttctattcgg aggccgggct gatcgtctcg tcccagaaca agggcagggg ccggtttgga
     2641 gctgccaaac aatcgacggt ggccgtcgcc ctggggctca agtaccgcat ctggacggtc
     2701 tcgggagagc aggctgtgga ggccgatccc gccggcctgt tttctcccgg cgacgtggtc
     2761 cggctgggcg tggaagtaaa cgacgcgggc tacctttacg tcgtccagtg gcagcctggc
     2821 ggtgcatgga agcgcctgtt tcccacaccg gagatcgagc aaggcagcca gtttgtgcgg
     2881 agcggcatcg tttactccat tcccgccggc ggccggaggc tggacttctc cgccggcctg
     2941 gaacggattc tgattctatt tgcccgccag cctatccggg agctcgagcc tttgatcgcc
     3001 gctcccggca gggccggctc ggatcccgtg gtggccgatg ccatcttgga aaaagcccgc
     3061 aagggaattg acgaaagagc gctggtgacc gagacggctg cttcggaaaa atgcgtgtac
     3121 gtagtaaacc ggaccggcaa gccggattcg gctttggcag ccgagatccg gctgagctcc
     3181 cgctgaccgg ccgatcctgc ccggccgggg gccctcttaa gtccctgata gttccgccga
     3241 ttctacttgg tgagagcgca tgatcagtgt gcgccgccgg ggcccggagc cgtaatgcgg
     3301 gcggcggtgt gttgctgggc agccgcctga agcgccgttg ctccggcccc gaccggaaac
     3361 ttatgccttc ctaccggtat tttcaggccg cgcctccgcc gcccaaggcg gttgactatc
     3421 gggccgatct gctggcggcg atcgaagact tgccgccctt gccgctgatc ctgaaccggc
     3481 ttctccagtt gctgaacgac agcaacgctt ccagcgcgca aatcgccgcg atgatcgaaa
     3541 aagacgcggt tttgagcgga agcgtgctgc gcagtgtgaa ttccgcttat tacggcctga
     3601 aggcgactgt aacttccatt cgccacgcgg tctcgatgtt ggggttttcc acggttcgta
     3661 atctggccct cgcgttttcg atgcggcgca tgctcacgcg ctcgcgaacg ccgccgccca
     3721 ggctgtacgc ccgctattcg cagcactccc tttcctgcgc catgctgtgc cactatctgg
     3781 cgggatacac cgggctgaac gattcggaag ccggtttcgc cgccggtctg tttcatgata
     3841 tcggcaaatt gctgatcctg acctccttcc ccgacttgct gcctaaagtg atcgaacatt
     3901 atgaacagtc ggaatgcagt tacgagcaag cggagatcga cattcttcag gtgagtcact
     3961 cggagctggg aagaatcgtg ttgcagaaat ggcaactgcc cgagtgcatc cagcaggccg
     4021 tggaatttca tcaccatcct gcggattgcc cccgggagtc cggcgccatt acgctggcgg
     4081 acattgtgca tgccgctgac atttacgtgg tcgagtacgg gctggacatt cttcccacca
     4141 agcggggtca tccacatccg gccgacatgg cctttgccga gatcggactc cggcagaaaa
     4201 tgcccgaggt tctcgaaaaa ttcaaaaacg aatacgaaag catccgcagc ctcttttaga
     4261 gggcaatggc gccgccgccc gcgccggctc ggccgcgtct tccccacaag catttccatt
     4321 tgaggcaaga tctgcgccgg cccgcctttt tcggccggca aatccacagg cagaccctcg
     4381 ccgattccgc gcccgggtac cattaccctg tcttgcagcc gtaccttgac ttcttgccca
     4441 ttggtttcca ttcgccgttg ggcggaagat ggggtttccc gtaaggacgg cttaaattga
     4501 acgctctact tcagttttct tatttgcagc ttctggacgt cctgaccacg ctggcgtttc
     4561 ttgcgaatgg agtcaaggaa gcgaatccgt tggtccggtt cttgctgaaa gcggggcctt
     4621 ctccgctggc cgcgctgatt ctcgtgaaag ggctggcgtt tgctctggcc atttattgcg
     4681 ttcgcaactc ccgccagcgg ctgctttcgc gtgtgaacat gggcttcgcc gcgctggtca
     4741 tgtggaatct ctgcattcta gtgatcagcg ggcctcaaat atctggttga aagtctcccg
     4801 ggttctttgc cgcatttcgg ccagaagctg atccatgggc cggccgtgga gcgattgcgg
     4861 ggtccagcgg cgcaccagtt ccgtcacaat ctcgtattgc gaacgcgatt tgggaagcat
     4921 tccgagcgat cttcccgtgg aaacgcgcag cgcatggtcc agggcgcggt agaaaacggc
     4981 cgcagtatga agaaattccg cattttcccg gctgagcagt cccattcttt cgattacgtc
     5041 gattcgttcc ggcgtgttca gggaggtata gaaaataccc gcccctctga gccgcaagta
     5101 catcagcacg aaatcgatat cgaagtagcc tccggggccg gccttcagcg gatgctcggc
     5161 gccctgctcg cgttcgagct tccggcgcat ttcgctcaac aggccggcca ggctcccgct
     5221 ttggccgtag cggcgccaat cgacgttttg cagctcgtga agaaacgccg tgccccgctc
     5281 gatgtcgccc gccaagcagc gggatttcat ataggtgatc gcttcccacg cctgcgcctg
     5341 accgctgaaa tatttgctgt attgcccctc ggtctggacc agttctccct cgcggcctaa
     5401 gggacgcagg cgggtgtcaa cggaaaagat gacgccttct ccggtatagc tggtgagcac
     5461 ctcgatggtg cgctcggcga cgcgggtcca gcggtcgagc tgcggcttgg cctcgtccgg
     5521 gacgataaag accagatccg cgtcagagcc cagatcgaat tcccgcatgc cgagccgccc
     5581 cagcgctacg acatgcatga gcgtagaggg cgcctcgtgc agcacttcgt ccagcgcgat
     5641 ccggtacgct gcgcgcacga cccagtcggc gagttccgtg gtctgctcga gcgtggcgaa
     5701 gatggggctg cggcggcaaa tgctttctgt ctgaatccgc agcatctgcc ggcggtaaaa
     5761 cctgcgcagc caggccgtct tctcgttgac gggggcgtgt gtgcgaagca aatgctcgag
     5821 ctcaggatgg tcgccgggaa aattgcgccg cggagcttcg agcgggaaca aaggcgcctg
     5881 ctggtcggag gcggaaaaag cggccacttc cagcaactgg tggatctcct ccgggttgcg
     5941 aatcagccat tccgcaagga aggggctgtg ctcgaacagg tccacggtca aatctaccag
     6001 ttccggctgc tcctccatgg ccgtcagcag atccggcatc gagacgatct tgccgagaaa
     6061 atgctcaaat aagccgcggc cgcgtttgag cggagcttgt gcaattcgcg ccgccacggc
     6121 cggcgctctc agctcgacat atcgaagctg cgcttgcata ccgcgctcca ggttttcctg
     6181 cggggcctcc atttccaatt ggaacagcgg cgccgcatca ggcccctgca aagagacggg
     6241 actatgtccg tgaatcacac ggtcatagat ttcagccacc gctcgcaggt gctgttcggt
     6301 gcgctcgacc agcgtgccgg ctattttttc gccgttgggc acgccgacct tgaagcccag
     6361 gacctccagc gcctccggct catccggaag cgtgtgggtc tggcgatcgt aatcgaactg
     6421 aagacggtgt tccacgatac gcaaaaaatg gtaggcgctg tttaggcggg cgtagtcgcg
     6481 cgccgagatg tagcgcttgt cgcgaagcct ttgcagagcc accagcgtgc cgccatgccg
     6541 gacccaggcg tcgcgtcccc cgtaaagccg ttgcaggcac tgcaccagga attcgatatc
     6601 gcgaataccc ccgcggctca gtttcacgtt caatccgccg ggggcgcggc ggcgcagttt
     6661 ttcatgaatg cgttcgcgag tttccgaaac gctttcaatc gctgagaaat cggtcgttgt
     6721 ccggtagatc agcggctcga cgaagcgcaa aaaccttcgc ccgagttcgc ggctcccagc
     6781 cgctactcgc gctttgatca gcatttgcaa ttcccaatcg cgggcgcggc gctggtaata
     6841 atcccgcaag ccgctcagcg gctgcgccac ttcgcccaga ttgccctccg ggcgcagccg
     6901 caggtcaacc ctgtagcagg agccttccgg cgtgttcgag gaaagcagat cggtgagctg
     6961 gttggccacc ttgatgaaga attccttgtt ggttagcctt ttgggcccgt ccgtctcccc
     7021 gccgccggcg aagaaataca tcaggtcaat gtccgagctg taattgagct cccggccgcc
     7081 gagcttgccg agagccaata cggtgaactc gcaaagctgg ccgcccccgg cccgcttctc
     7141 cggagaggcg gcggcgcccg ctggagccag ccggggcgaa ccaaaaattt ccgtcaactc
     7201 cgcttgaatc cgctccaggg ccgcttccag aatcgtgtcg gcgagattcg acagttccag
     7261 ggtcgtctcg gccagtgtgc tgaggcccaa aacatcgcgc agcatgatgc ggagcagatt
     7321 gcgcctccgg aaacgcgcca gctcgagtgc aacagcctcg cggcgcatct cgcgagggaa
     7381 cgggcctaat tgggatcgca gctcttcggg cccgagcacg cggtagaagc cgttttgttc
     7441 agagacgcaa tccagccagt cggggtgctg gaatatggct tccgcgagga agtggctata
     7501 agagaatacg gctaagagag cgtttagccg ggaggtccga gcgagggtgc cggcggcctc
     7561 tgccccgctc gacagaaacc ggtcgaggta atgaatcgcc gcatccggat ccggcgactg
     7621 cgcgagcagc gaattcagta tgttttcggt ttccgaactg agataacggc tcagacctgc
     7681 caggctgcgc tgcgctcgct cgacgtggcg aaacggaacg ctctccagga ggcggtccat
     7741 agaagcgggc ctgtggattg tgggctgtgg ctcacgcgcg gtggagacca caagccattc
     7801 gccacaggcc acaaccacta gatatcgtaa tacatggcga actcgtacgg gtgcgggcgc
     7861 agccggatcg cgtcgacttc gttcttcatc ttgtaatcga tccaggtttc gatgatctct
     7921 tctgtaaaca cgtcgccttt ggtcagaaac gcgtggtcgt tctcgagggc tttcaaggcc
     7981 tcgtcgagcg aagccggcag cgacggcacg cctcgcattt cttcgggaga aaggtcgtag
     8041 atgtccttgt ccagcggctc gccggggtcg atcttgttct ggatgccgtc caagccggcc
     8101 atgagcatgg cggcgaaagc caggtacgga ttgcaggaag gatccggagg ccgaaactcc
     8161 acgcgcttgg ctttggggct ggccgagtac atcggaatgc ggcacgcggc cgaacggttg
     8221 cggcgggagt acgcgagatt gaccggcgct tcgaagccgg gaaccaatcg tttgaagctg
     8281 tttgtcgtgg gagcggccag cgctacgacc gccggcgcgt gcttcagcag cccgccaatg
     8341 taccacaaag cgatatcgga aagaccggca tagcggtcgc cggcgaacag gggcgtgccg
     8401 ttcttccaaa tggactggtg cgtgtgcatg ccgctgccgt tatcctggaa caacggctta
     8461 ggcataaacg tgacggtctt gccgtgctgc gccgcaacgt tccggatgac gtacttgtaa
     8521 atcatcatgt tgtcggccga tttcaccagc gaatcaaagc gctggtcgat ttcggcctgg
     8581 ccgccggtag ccacctcgtg atgatggcac tcaatttcca ggccgctctt caacatcgtg
     8641 cgcaccatct cggcccgcag gtcctgatag tgatctgtag ggggtaccgg gaagtagcct
     8701 tccttgtagc ggggccggta tccaagattg ttctccgaac gcccggagtt ccaccgccct
     8761 tcctcggcgt cgatgaagta atatccgctg ttctgggtct gatcaaaacg aatgttgtcg
     8821 aagatgaaaa actccgcctc ggcgccgaaa taggcgatat ctccgatgcc gctgtacttc
     8881 aggtagtttt ccgccttctt ggcgatccag cgcggatccc gctcgtaatg ctgcttggtg
     8941 atgggatcga tcacgtcgcc gatcatcacc agcgtgggaa tctgcgtaaa cggatccatc
     9001 atgcaggtcg aagcatcggg aatcagcagc atgtcgctct cgtgaatagc tgcccacccg
     9061 cgcaggctcg atccatcaat gccgaagccg tcctcgaagc tgctctcggt caattgcgcg
     9121 atcgggaaag aaacatgatg ccagagaccc ggcagatcgc tgaagcgcat gtccagcatc
     9181 tgcgcgccat tggccttggc aaactccaac acctctttgg gacttcttac aatactcatc
     9241 acacctccag tgtttatgcg ggcaaagaat ttaaggggga taccgcaatt acagaaccgt
     9301 taatggtagc ataacagtcc tttgcggcga ggctggcttc cggaaggcgc ggccggtgaa
     9361 gtgacgccgg cttggatgta aagacttccg gtcacggcag cagcttccgg taccggccgg
     9421 aggcaaccgg ctgcgcccga gcatgaggag gcggcacgcg ccggcttcgc tcgattcctc
     9481 cgcccgagcg agaattttta tcgccctttc ccgaagagca aactgcaaca cggcctgatc
     9541 cggcgattgg cgttagcccc gggtggcttt cggcgccgac gcgtatacct cgctggccgg
     9601 cgccggagta gttcttctaa ggggccgctg gggaccacct gcaagtcgcc gcaccccgca
     9661 ggaactccgg acgagcctct cggatcaggg tgcgt
//
LOCUS       DASREK010000180         7909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_584078, whole genome shotgun sequence.
ACCESSION   DASREK010000180 DASREK010000000
VERSION     DASREK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584078"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1861)
                     /locus_tag="VFA54_09285"
     CDS             complement(<1..1861)
                     /locus_tag="VFA54_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZS51040.1"
                     /translation="MKIGIDLGTTNSALAYIDEREAEDRDFPPVHIFETPQQVAAGRV
                     EPRRTLPSFLFLEDGQPVGVYAREQGALVPTRLVHSAKSWLSNPDVDRTAKILPWDSP
                     ETGRVLSPVEVSARYLAKFREEWDKTKAGPLAEQDIVLTVPASFDEEARELTVMAARD
                     AGIERLTLLEEPAAAFYSWIANNLAQSRKKLFDGQIVLVCDVGGGTSDFSLIRVSREG
                     DLVNFTRTAVGKHLLLGGDNLDLTFAWLVETKLGAQLSIRQRSGLRRQCSSAKEILLT
                     DPTRQSVEITVLGSGSALIGKTLKSEIRREEALELALEGFLPFTERGEGPKEEKRSLF
                     RELGLPYVSDAAITRHLNAFLESAGQAPDAILFNGGFFIPEILRERVAEVVGRWYGRR
                     PEILENTDLDLAVARGAAYYSYVRATGSGVLVRGGLPRTYFIGISEPREGKISAVCLV
                     PRGAEEGTALEIDNDALQLVANRPVSFRLYSSLTRAEDKLGDVVEFPADDPGLHMHAP
                     LHAVIRFGKKAEERLIPVKLGARLTEIGTLESWCESKISDNRWRLQFELRKAAPQHPA
                     ERKAAAVISEQSLKASLELITNVFSPSAKSPITPEEVPAKLEQTMGLGKNSWPL"
     gene            complement(1957..3810)
                     /locus_tag="VFA54_09290"
     CDS             complement(1957..3810)
                     /locus_tag="VFA54_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZS51041.1"
                     /translation="MKSSRSQLEAKYVIGIDLGTTNSALSYAEIRADADPFAPANVQV
                     MSIPQLTNPGEVRDEDLLPSFLYLPGSSDFPAGALALPWDDTRDFAVGVLAQKRGTEN
                     AGRLVSSAKSWLSHSGVDRTAPLVPFRAPEGVDKISPVTASRRYLEHLREAWDHRMPD
                     FPFTEQQVLVTVPASFDAVARELTLEAAQKAGYQNLTLLEEPQAAFYAWIERHSDWRD
                     RVQVGDLILVVDIGGGTTDFSLIAVTEHEGELALERVAVGEHILLGGDNIDLALAGAV
                     SQRLAEKGTRLDSRQMQTLWNNCRTAKEKLLTPGSKSKEEPVTILGKGTGLVGGTIKA
                     SLPREDIDRILSEGFLPSVASADMPVRQRRAGLQEIGLPYAADPAITKHLARFLRQQA
                     ATSEHGAVRRGPSGLACPTHVLFNGGVLRAGMVRERLLATLNSWLAEEGMGAVKPLSG
                     EDLMHAVARGASYHGLARRGRGVRIRGGVPRTYYVGIESAMPAAPGFAAPLKALTVVA
                     FGMEEGTDVRIPGREFGLVVGEPAEFRFFASAARKNDQPGDLIEDFAGELEELSPMEV
                     TFGAGESSAEVVPVSFETVVTETGLLQLWCVARDNRRWKLEFNVRERVAAA"
     gene            complement(3797..4153)
                     /locus_tag="VFA54_09295"
     CDS             complement(3797..4153)
                     /locus_tag="VFA54_09295"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HZS51042.1"
                     /translation="MLGILQRDSRLIDFLMEDISAYSDDQVGAAVRELHNQCRDSVAR
                     YVTLQPVIDSVEGTFTQAPSKDPNLVKFIGNVPATPPSGGTLRHKGWRAVKVDLPALG
                     AKQDASVLAPAEIEIE"
     gene            complement(4375..4611)
                     /locus_tag="VFA54_09300"
     CDS             complement(4375..4611)
                     /locus_tag="VFA54_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51043.1"
                     /translation="MDLSEIIRTLYVEKEKVENSIAALEALHATHGDGPERARRRGRK
                     SMGDEERQQVSERMKKYWGSRRQQQTALEAVSSN"
     gene            complement(4731..5636)
                     /locus_tag="VFA54_09305"
     CDS             complement(4731..5636)
                     /locus_tag="VFA54_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS51044.1"
                     /translation="MISNAPRQEYTRAEVRRQFGLTERQLKRWEHDSLLPAAESYSFS
                     DLIAIRTILELHGKGFRSRKIAEAVEALRHKLDGITQPLSELRILSDGKKIAVRLAGQ
                     KMEAVSGQTLFDFEASELGGLKDFALRKRPGNRLRESEAWFQKALALEETGAPLEDIL
                     EAYEKVVELNPAAAGALVNLGTIHYRQRNFPEAERYYKDATTADPTYPLAEFNLGNLY
                     DEQGRLDEAFDHYRRSLALNPHYADAHFNLALLCERTGESLRAVHHWKCYLKLDRSGP
                     WAEIARRQLDRLHQVTVIPSRKRRE"
     gene            complement(5646..7304)
                     /locus_tag="VFA54_09310"
     CDS             complement(5646..7304)
                     /locus_tag="VFA54_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51045.1"
                     /translation="MPEPGTQADVGELLRRIREIEARLARLEQRFEPAPSASAPRAEP
                     AQIALIESVAAVPILGRAVLAMAGAYVLRAFTEAHMVAPKAGVFAAIGYAVFWLVWAA
                     RIPAGRRAETALYSLTSALVLGPLIWEATLRLHAVSTWTSAAVLLAFTSIGLLISWRK
                     NLLIVATISILTAVLTGAALLIGSHDVVPFLLLLVLIAAAVEISACLDHWLSERWLAA
                     AAADLVVLLATYLASDPRGLPESYAPIPYFFVLGAQLALPAIYLSSTVVRTFLRGSTI
                     RMFEVAQLAIVFLLVLGGAPRLGGPSILPAISVFCLGSAAVCYATGAALKGRNFQVYL
                     GFAFLLTLAAVRIALPAGWAAAVTSALALGCMMLPRPAFRWQGAGYLLFALGSSGALA
                     QSTRGLLDGHDPTDSLLPLLGEAAIALACFILAARGPSPSVLRMALAAAAFWPLAALG
                     AAAIAGSYHAIFGSLASHSYCAAGRTAVLAGGAVLLAWIGSRQTAFEITPLVYPLMIL
                     GAYRLLLVDLRQDSKAALVISLLVYGSALLLVPRFMRPRQTTSS"
     gene            complement(7315..>7909)
                     /gene="nrfD"
                     /locus_tag="VFA54_09315"
     CDS             complement(7315..>7909)
                     /gene="nrfD"
                     /locus_tag="VFA54_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZS51046.1"
                     /translation="PLMPIVFLFSAMTSGIAAVLVLYVISMKLRKTALDMRCLDTIAK
                     YLFYTFVIDFTLEMLDLVQRTYEADESFRSLDFMVHTRLWTSQVLIQIFLGTLGPLCL
                     LAATQLWKFSEPVRRRLYVTASCLTLIGIFAMRWNVVIGGQLFSKSFLGYTTYKVQFA
                     AREGLLPAIMLMILPFVLFFVLVKLLPPWQERTTTEA"
BASE COUNT         1588 a         2300 c         2629 g         1392 t
ORIGIN      
        1 aaagcggcca cgagttcttg ccgagcccca tggtctgctc gagtttcgca ggcacctctt
       61 cgggcgtgat cggagatttg gccgagggag aaaagacgtt ggtaatcaat tcgagcgatg
      121 ccttcaggga ctgctcactg atcaccgccg cggctttgcg ttccgcaggg tgttgggggg
      181 cggctttgcg caattcaaac tgaagccgcc agcggttgtc gctaatcttc gattcgcacc
      241 aggattccag tgtgccgatt tccgtcaggc gggcgccgag cttgaccggg atgagccgct
      301 cctccgcttt ctttccgaaa cgaatgacgg cgtgcagcgg agcgtgcatg tgcaagccgg
      361 gatcatcggc cggaaattcg acgacgtcgc cgagcttgtc ttcagcgcgg gtgagcgagc
      421 tgtacaagcg gaaggagacg ggccggttcg ccaccagttg cagcgcgtca ttgtcgattt
      481 cgagcgccgt gccttcttcg gcgccgcggg ggaccaggca cacggcggag atcttgccct
      541 cgcgcggctc gctgatgccg atgaaatagg tgcgcggcaa gccaccgcgc accaagacgc
      601 cgctgccggt ggcacggacg taggaatagt atgccgcgcc gcgggcgacc gcgagatcca
      661 gatcggtgtt ctcgaggatc tccgggcgac ggccatacca gcggccaaca acctcggcga
      721 cgcgctcgcg cagaatctcg gggatgaaga aaccgccgtt gaacagaatg gcgtcgggcg
      781 cctgaccggc gctctcgagg aaggcgttca ggtggcgcgt gatggcggca tcggagacat
      841 agggcagacc cagctcgcga aacaggctgc gcttttcctc cttcgggcct tcgccgcgct
      901 cggtgaacgg gagaaagcct tccagagcca gctcgagcgc ttcctcgcgc cggatttcgc
      961 tcttgagggt tttgccgatg agggccgagc cggaaccgag cacggtgatt tcgacgctct
     1021 ggcgcgtggg atcggtgagc agaatttcct tggccgagga gcattggcgg cgcaggccgc
     1081 tgcgttggcg gatggagagc tgagcaccga gcttggtctc gaccagccag gcaaaggtga
     1141 gatcgaggtt atcgcctccg agcagcaagt gcttgccgac ggcggtgcgc gtgaaattca
     1201 ccagatcgcc ttcgcgcgag acgcggatca ggctgaaatc gctggtgccg ccgcccacgt
     1261 cacagacgag cacaatctgt ccatcgaaca gctttttgcg cgattgggcc aggttgttgg
     1321 cgatccagga ataaaatgcg gcggcgggtt cttcgagcag tgtgaggcgc tcaatgccgg
     1381 cgtcgcgcgc ggccatgaca gtcagttcgc gagcctcttc gtcgaaggat gcggggacgg
     1441 tcaggacgat gtcctgttcg gcgaggggcc ccgctttggt cttatcccac tcctcgcgaa
     1501 atttcgcgag atagcgcgcg gatacttcca ccggagaaag gacgcgcccc gtttcgggcg
     1561 aatcccaggg gagaattttc gccgtgcgat cgacgtcagg attcgatagc caggatttgg
     1621 cggagtgcac caggcgcgtg gggaccaggg cgccctgctc gcgggcatag acgccgacgg
     1681 gctgaccatc ttcgaggaac aggaaggagg gcaacgtgcg gcggggttcg acacggccgg
     1741 cggcgacctg ctggggcgtc tcgaaaatat ggacgggagg gaaatcgcgg tcttcggctt
     1801 cgcgctcgtc aatgtaggcc agggcagagt tggtggtgcc caggtcgatg ccaattttca
     1861 tgtcgtcatc tgtctttcag ggtaaccccg ctccttatgg ccgcgactct atatcggacg
     1921 ccagagacag ggccatcacg aaggagcgga caatctttac gccgcggcta cgcgttcgcg
     1981 gacgttgaat tcgagtttcc agcggcgatt gtcgcgggcc acgcaccaga gctgcagcag
     2041 gccggtttcg gtaactacgg tctcgaacga gacggggact acttcggcgg aagattcgcc
     2101 ggccccgaag gtcacttcca tcggggagag ctcctcgagt tctccggcga agtcttcaat
     2161 cagatcgcca ggctgatcgt tttttcgggc ggcggaggca aagaagcgaa actccgcggg
     2221 ttcgccgacg accaagccga actcgcggcc gggaatccgc acgtcagtgc cctcctccat
     2281 accgaaagcc acgactgtta acgccttgag gggagcggcg aatccgggag cggcgggcat
     2341 cgcgctctca atacccacat agtatgtccg ggggacgccg ccacggatgc ggacaccgcg
     2401 gccgcgccgc gccagaccat gataggaggc tccgcgagcc accgcgtgca tcaggtcttc
     2461 gccgctcagc ggcttgacgg cacccatgcc ttcttccgcg agccacgagt tgagggttgc
     2521 gagcaggcgt tcgcgcacca tgccggcgcg caagacgccg ccgttgaaca ggacatgcgt
     2581 ggggcaggcc aaaccgctcg gcccgcggcg caccgcaccg tgttcggagg tcgcggcctg
     2641 ctggcggaga aagcgagcca ggtgtttcgt gatcgcggga tcggcggcat acggcaggcc
     2701 gatttcctga agcccggcgc gccgctgacg cacgggcatg tccgcgctcg caaccgacgg
     2761 cagaaagcct tcactcagga tgcgatcgat gtcttcgcgc ggcagcgagg ctttgatcgt
     2821 gccgccaacc aggccagtgc ccttgccgag aatcgtaacc ggctcttctt tggatttcga
     2881 gccgggcgtc aataactttt ctttggcggt gcggcaattg ttccagagcg tttgcatttg
     2941 gcggctgtcg aggcgtgtgc ctttttcggc cagccgctgc gagacggcgc ccgccaatgc
     3001 gagatcgatg ttgtcgccgc ccagcagaat atgctcgccc acggccacgc gttcgagcgc
     3061 caattcgccc tcgtgctcgg tgacggcgat caaggagaaa tcggtggtgc cgccgccgat
     3121 atccacgacc aggatcaaat cgccgacctg gacgcggtcg cgccagtcgg agtggcgttc
     3181 gatccaggca tagaaagcag cctgcggttc ttcgagcagc gtcaaatttt gatagccggc
     3241 tttctgggcg gcttcgagcg tcagctcgcg agctacggca tcgaaggagg ctggcacggt
     3301 caccagcacc tgctgctcag tgaagggaaa atcgggcatg cgatgatccc aggcctcgcg
     3361 caagtgttcg aggtagcgac ggcttgccgt gactggggaa atcttgtcta cgccttccgg
     3421 cgcgcggaag ggcaccaggg gagcggttcg gtccacgccg gaatgcgaga gccaggattt
     3481 tgcggaggaa accaggcggc cggcgttttc cgtgccgcgc ttttgcgcca ggaccccgac
     3541 ggcgaaatcg cgggtatcgt cccagggcag ggcaagggcg cccgcgggga aatccgagga
     3601 gccgggcaga tagaggaagg agggcagcag gtcttcgtcg cgcacctccc cgggattcgt
     3661 gagctggggg atgctcatga cctggacgtt ggcgggcgcg aacggatcgg catccgcccg
     3721 gatctccgcg taagacagcg cggagttggt ggtgccgaga tcaattccga tgacgtattt
     3781 ggcttccaat tgggatctac tcgatttcaa tttccgcggg cgccagcacg gacgcgtcct
     3841 gtttggcgcc gagagccggc agatccacct tcacggcacg ccagccttta tggcggagag
     3901 tgccgccgga gggcggggta gccggcacgt ttccgatgaa cttcacgaga ttcggatcct
     3961 tggaaggggc ctgggtaaag gttccttcga cgctgtcgat caccggctgg agggtgacgt
     4021 agcgcgccac cgaatcgcgg cattgattgt gcaactcgcg gacggccgcg ccgacctgat
     4081 cgtccgaata cgcgctgata tcttccatca gaaaatcgat gaggcgcgaa tcgcgctgca
     4141 gaattcctag catctggagg gcaccgtccg aggttttggc ggtgggggca gccggggggg
     4201 ccggcttcgc cggggtggct ggacccgcgg tcgagcgccg gctcaagccg agggcggtca
     4261 tcgccgaatc cgacaggcgg ccgtggaata gaaggttaaa gaaacaactg aacgcgagaa
     4321 aaagacgatt caacgcttta gctccagaaa ctagaatagc gtgtcaacac gcttctagtt
     4381 ggagctgacg gcctccaagg cggtctgttg ctgccggcgg cttccccagt actttttcat
     4441 gcgttcggaa acctgctgcc gttcttcgtc acccatcgat ttccggccac ggcgccgtgc
     4501 gcgctcgggc ccgtccccat gggtagcgtg gagcgcttcc agtgccgcga tgctgttctc
     4561 cactttttcc ttttcgacgt acagagttct aatgatttcg ctcaagtcca taattttctt
     4621 ctaggaatgt aagaatcagt acatccggat tggagcagcc agcaagattg ctcgcaagcc
     4681 atcggtaaag ttgtaatcgg aaacaaggaa ttccgcatca cccaaaaggg ctattcgcgg
     4741 cgcttacgag aaggaataac ggtcacctgg tggagccgat cgagctgacg tcgtgcgatt
     4801 tcagcccagg gaccgctacg gtccagcttc aaatagcatt tccaatggtg caccgctcgg
     4861 agggattcgc ccgtcctctc gcaaagtaaa gcgaggttga aatgggcatc ggcgtagtgg
     4921 ggatttaatg cgagggagcg ccggtagtga tcgaatgcct catccaagcg gccttgctcg
     4981 tcatatagat tgccgaggtt gaactcggcc aagggatagg tgggatcggc ggtagtcgcg
     5041 tctttgtagt accgttcggc ctcgggaaaa ttgcgctgac ggtaatggat cgtacctaaa
     5101 ttaaccaggg cgccagcagc cgcaggattc agctcgacga ccttctcgta ggcttcaagg
     5161 atgtcctcca gaggtgcgcc ggtctcctcg agcgccagcg ccttttggaa ccaagcttcg
     5221 gattcgcgca agcggttgcc gggccgcttt cggagcgcaa aatctttgag gcctccaagt
     5281 tcggaggcct cgaaatcaaa cagcgtctga ccgctgacgg cttccatttt ctggccagcc
     5341 agccgcaccg cgatcttctt accgtcggaa agaattcgca attcagatag cggctgcgtg
     5401 atgccgtcca gtttgtggcg gagcgcctcg acggcttcgg cgattttacg cgagcggaag
     5461 cctttgccat gcaactcgag gatggtgcga atcgcgatca ggtcggagaa cgaataggat
     5521 tcggccgcgg gcagcaacga atcgtgttcc caacgtttca actggcgctc ggtaagaccg
     5581 aattggcggc gcacctcggc acgagtgtat tcctgccgcg gcgcattcga gatcacggga
     5641 ccattttagc ttgatgtggt ttgacgaggc ctcatgaaac gtggaactag caagagcgcg
     5701 gagccgtaga caagcagcga gatgaccagc gccgccttgc tgtcctggcg caagtcgacc
     5761 aggaggaggc ggtatgcgcc gaggatcatc aacgggtaga ccaggggcgt gatttcgaag
     5821 gcggtttgac gagatcctat ccaggccagc agcacggcgc cgccggccag tacggccgtc
     5881 cgcccggcgg cgcaatagga gtgcgacgcg agactcccaa aaatggcgtg gtatgaccct
     5941 gcgatagcgg cggcgcccaa tgccgccaga ggccagaacg cggcagcggc taacgccatt
     6001 cgaaggacac tcgggctggg gccgcgagcg gccaggatga agcaggcgag cgcgatcgct
     6061 gcttctccga gaagcggcag caaggagtcc gttggatcgt gtccgtcgag gagccctcgg
     6121 gtggattgcg ccagcgcgcc ggaagagccg agggcaaaca gcaggtagcc ggcgccctgc
     6181 cagcgaaacg ccggcctcgg tagcatcatg catccgagag cgagcgcgga ggttaccgcg
     6241 gccgcccatc cggccggcag agcgattcgg accgcggcca gggtgagcag gaaagcaaat
     6301 ccgagataga cctgaaaatt gcgacctttc agagcggccc ctgttgcata acagacggca
     6361 gcggagccca ggcagaagac ggagatggct ggaaggatcg acgggccgcc cagccggggc
     6421 gcgcctccga gcacgagcag gaagacgatc gcaagctgcg ccacttcgaa catgcggatg
     6481 gtcgagccgc gcaggaacgt gcggacgacg gtgctcgaca gatagattgc cgggagtgcc
     6541 aattgtgcgc ccagaacgaa gaagtaaggg atgggggcgt agctctcggg cagcccacgt
     6601 gggtcgctcg cgagatacgt cgcaagcaag accaccagat ccgcggcagc ggcggcgagc
     6661 caacgctcgc tcagccagtg gtcgagacag gctgaaatct cgacggcggc ggcaatcagg
     6721 accaggagga ggaggaatgg gacgacgtca tgcgagccga tcagtagggc cgcgccagtc
     6781 agtaccgcgg tgagaatgga aatggtggcg acgatcagca gattcttgcg ccaggaaatg
     6841 agcaatccga tggacgtgaa ggcaagcagg accgccgcgc tcgtccaagt cgaaaccgca
     6901 tgcagacgga gcgtggcttc ccagatgagg gggcccagga ccaaagcgga ggtgaggctg
     6961 tagagcgccg tttccgcgcg gcgaccggcc gggatacgcg ccgcccagac cagccagaac
     7021 acagcatagc caatcgcggc gaatacaccg gctttcggcg ccaccatgtg ggcttcggtg
     7081 aacgcgcgga gcacataggc gccggccatg gcgagcaccg ctcggccgag aatgggcaca
     7141 gcagcgacac tctcgatcag cgcaatctgc gcgggctcgg ccctcggtgc gctcgcgctc
     7201 ggagccggct cgaatcgctg ttcgaggcgg gcgagacgag cttcgatctc ccgtattctg
     7261 cggaggagtt ccccgacatc ggcttgcgtg ccgggttccg gcatcgcgcc ctcctcaggc
     7321 ttcggtggtt gtccgctcct gccacggcgg cagcagcttc accagcacga agaacagcac
     7381 aaacggcagg atcatgagca tgatcgccgg cagcaacccc tcgcgcgcgg caaattgcac
     7441 cttgtacgtg gtgtagccga gaaaactctt ggagaataat tggccgccga tcaccacgtt
     7501 ccaacgcatg gcgaagatgc cgatcaaagt caggcagctt gccgttacat acaggcggcg
     7561 gcgcaccggc tcggagaatt tccagagttg cgttgcagcg agcagacaca aggggccgag
     7621 cgtgcccaga aaaatctgga tcaacacctg cgacgtccag agccgcgtgt gcaccatgaa
     7681 atcgagcgaa cgaaacgatt catcggcctc ataggtgcgc tggaccaggt cgagcatctc
     7741 gagcgtgaaa tcgatgacga aagtgtagaa aagatacttg gctatggtat ccaggcaacg
     7801 catgtcgagc gccgttttcc gcagtttcat ggagatgaca tagaggacca gcacggcagc
     7861 gatcccggag gtcatggcgg agaacaggaa aacgatcggc atcaggggc
//
LOCUS       DASREK010000181         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1413934, whole genome shotgun sequence.
ACCESSION   DASREK010000181 DASREK010000000
VERSION     DASREK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413934"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VFA54_09320"
     CDS             <1..975
                     /locus_tag="VFA54_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZS51047.1"
                     /translation="PGEPGSETWKDGKNFWQFGGGSTWGTGSYDPELNLVYWGTGNPG
                     PDLNSDVRPGDNLYTNSMVALDADTGKLKWHFQFTPHDTHDWDSISEPILADVNINNT
                     PRKVLLQANRNGFFYALDRTNGKFIYGFPYVHQTWAKGLDQNGRPIVVPGMEPTKEGK
                     VVCPSLGGGKNWNHAAYNPQTGLMYVPSAEGCEKFFIDDLPEPEPFRMWMGSIHETIG
                     TTPQWGAVRAYDVRTGKLAWEFKTITPERGSALTTGGNLLFIGDSQGYFTAFDATNGK
                     VLWKINTGAGSGRGGGVSAPAITYMLDGKQQIAVIAGQSLFAFKLLEP"
     gene            complement(1071..1397)
                     /locus_tag="VFA54_09325"
     CDS             complement(1071..1397)
                     /locus_tag="VFA54_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51048.1"
                     /translation="MKVPLLRLAHARSGDKGDTANIGLIAFEQRHYPILVREVTAERV
                     KSHFGELCRGEVQRFELPNLGALNFLLHRSLGGGGTVSLRLDAQGKTFGAALLRMEID
                     IDENEL"
     gene            1524..2330
                     /locus_tag="VFA54_09330"
     CDS             1524..2330
                     /locus_tag="VFA54_09330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HZS51049.1"
                     /translation="MPQETGYFSRRLEGRVALVTGASRGIGRAIAIRLALEGAAVAVN
                     FRSEAKAAGETVERIRSLGGQAFAVQADVADPASAGRLVAETCERFGGIDVLVNNAGI
                     LYRGDIFNYNAEEFYRMHAANTGGVANTAAAVAPRMKEKKSGSIVNITSIAALGTAMH
                     GTTFYAATKAAVIILTKRFALELGPFGVNVNAVAPGFIQTDMTFAGRTPEEAQQVSES
                     MKSRTMLGRVGKPEDIASVVAFLASPDARFVTGQILTADGGRLDFLTHGL"
     gene            complement(2543..3622)
                     /locus_tag="VFA54_09335"
     CDS             complement(2543..3622)
                     /locus_tag="VFA54_09335"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HZS51050.1"
                     /translation="MKKLNIGLLIIAAALFFGVCWAQQLKSKAPEQGWVPEAWYPHQG
                     SLLKYRVHIRFGEEPDTMPDGWKFGRVSAIATNQAGNEVFVFQRGTHADPLIVFDNKG
                     KFLRSWGKGMFNNPHGLRADKDDNIFVTDTTGQQVIKFDKSGRVLMTLGVKGQKGTDD
                     KHFNLPTDIAFARTGANYNGDFYVSDGYGNSRVVKFDKNGKYLLAWGTPGSGPGQFHT
                     PHSVAVDSKGRVYVSDRENNRIQIFTANGEFLKQWNHLGATQGLFITPDDNLWIITHR
                     NNVENITYDTLAGQIMHVDLETGKVLGAMESPGHEINVASDGQIYIASLTGNVFQWYP
                     GWLDRRTAANAEKNGPPGSSKKGRN"
BASE COUNT          823 a         1177 c         1206 g          820 t
ORIGIN      
        1 ccgggcgagc ccggctcgga gacctggaaa gatgggaaaa atttctggca attcggaggc
       61 ggatcgacct ggggtacagg gtcttacgat ccggagctga acctggtgta ctggggtacc
      121 ggcaaccccg ggcctgatct gaatagcgac gtgcggcccg gcgacaatct ctacaccaac
      181 tcgatggtgg ccctggacgc cgatacgggg aagctcaaat ggcacttcca gttcaccccg
      241 cacgacactc atgattggga ctctatcagc gagcccatcc tggccgacgt gaatattaac
      301 aacacgccgc gcaaagtgct gctgcaagcc aaccgcaacg gctttttcta cgccctcgac
      361 cgcaccaacg gaaagttcat ttacggcttc ccttacgtgc atcagacctg ggcaaaggga
      421 ctcgatcaaa acggacgccc gattgtcgtc ccgggcatgg aaccgaccaa ggaaggtaag
      481 gtcgtctgcc ctagcctggg cggaggcaag aattggaacc acgcggccta caatccccag
      541 accggtctga tgtatgtgcc ttccgcagag ggctgtgaaa aattttttat cgacgacctg
      601 ccggagcccg aacccttccg catgtggatg gggagcatcc atgagacgat cgggaccacg
      661 ccgcaatggg gggcggtgcg ggcttacgat gttcgtaccg gaaagctggc ctgggaattc
      721 aaaacgatca cacccgagcg cggctcagct ttaaccaccg gcggcaacct gctgtttatc
      781 ggggattcgc aaggttattt cacggcgttc gatgccacca acggcaaagt gctctggaag
      841 ataaataccg gcgcgggctc cgggcgcggc ggcggagtat cggcccctgc aattacgtat
      901 atgctcgatg gcaagcagca gatcgcggtg atcgccgggc agtcgctgtt tgcgttcaag
      961 ttgctggaac cctagccggc ggttcctggg ctactttttg gcgccttggt tctgtgaacc
     1021 ggccgaagtc cggcgccgca atggccggcc gcggaagcgg gcgccgctct tcacagctcg
     1081 ttttcatcga tgtcgatttc cattcgcagc agagcggctc cgaatgtttt gccttgagcg
     1141 tccaggcgaa gagagacggt tccgccgcca cccagggaac ggtgcagcag gaaattcaac
     1201 gcccccagat tgggcagttc gaagcgctgc acttcgcccc ggcaaagctc tccgaagtgg
     1261 ctcttcaccc gctcggccgt tacctcccgg accagaatcg gataatgccg ctgctcgaac
     1321 gcaatcagtc cgatgtttgc ggtatcgcct ttgtcgcctg agcgcgcatg tgccagccgg
     1381 agaagcggga ctttcataga aacactttta gcatggtccc gagcctagcg cgagcttata
     1441 aaatcggccg ccggcccggg gagggcgcgt gccggctccg gcgaggctgc tgagttgcac
     1501 gaattctttg ccgaggagac aaaatgcccc aagaaactgg ttatttcagc cgccggctgg
     1561 aaggccgtgt ggcgctggtg acaggagctt cacgcggcat cgggcgagcc atcgcgatac
     1621 gtttggcgct cgagggggcc gctgtggcgg tcaatttccg cagcgaagcg aaggccgccg
     1681 gcgagaccgt cgagcggatc agaagcctgg gaggccaggc cttcgccgtg caggcggacg
     1741 tggcggatcc tgcttcggcc ggccggctgg ttgccgagac ctgcgagcgt ttcggaggta
     1801 tcgatgtact ggtcaacaac gccggcattc tgtaccgggg cgacatcttc aattacaacg
     1861 ccgaagagtt ttatcgcatg cacgccgcca acaccggcgg agtcgccaat accgccgccg
     1921 cggttgcgcc gcgtatgaag gaaaagaaat ccggaagcat cgtcaatatc acgtctattg
     1981 ccgcgctagg aacggccatg cacgggacga cgttttatgc ggcaacgaaa gcggcagtga
     2041 ttattttgac caagcgattc gcgcttgaat taggaccttt cggggttaat gtgaatgccg
     2101 tcgctcccgg cttcattcaa acagatatga ccttcgccgg ccggactccg gaggaggcgc
     2161 agcaggtttc cgagagcatg aaatcccgga ccatgctggg ccgcgtagga aagccagagg
     2221 atattgcttc cgttgtcgcc tttctggctt caccggacgc aagatttgtc accggccaaa
     2281 ttctaaccgc cgacggcggc cgcctggact tcttgactca cggcttatag agccgcctca
     2341 aaccgccgcg gccacttgtg tgtaccgccg gcgatcggta ttgaccttgt attttgacca
     2401 cgcgaagagc ctgctggccg gagatacgaa tgccggcagg agccggagga cagcgatctc
     2461 tagccgccgc ggccgatcat ggagagacgc agaaatccga gacgcccccg caagcgggcg
     2521 cctcggagga atgtcagcgt ggtcagttgc gtcctttttt cgatgacccc ggaggtccgt
     2581 tcttttccgc attagcggct gtgcgccgat ccagccaccc cggataccac tggaaaacgt
     2641 tgcccgtgag gctggcaatg taaatctggc cgtcggaggc cacattgatt tcgtgcccgg
     2701 gactttccat cgcgccgaga accttgccgg tctcgaggtc cacatgcatg atctggccgg
     2761 ccaaggtgtc atacgtgatg ttttctacgt tgttgcggtg ggtgatgatc cataggttgt
     2821 cgtcaggggt gatgaacagc ccttgcgtag cgccgaggtg attccattgc tttaaaaact
     2881 cgccgttggc ggtgaaaatc tgaatccggt tgttctcgcg gtcgctgacg taaaccctgc
     2941 ctttggaatc cacagctacg gaatgtgggg tgtggaactg gcccggaccg ctccccggcg
     3001 tgccccaggc aagaagatat ttgccattct tgtcaaactt caccacacgg gagttgccgt
     3061 acccgtcgga aacatagaaa tctccgttgt aattggcgcc ggtgcgggcg aaggcaatgt
     3121 cggtgggaag attgaagtgc ttgtcgtcgg ttcccttctg ccccttgacg cccagcgtca
     3181 tcagcaccct tccgctctta tcgaatttaa tgacctgctg gccggtagta tcggttacaa
     3241 aaatattgtc atccttatcc gcccgcaggc cgtgcgggtt gttgaacatg cctttgcccc
     3301 aagagcgcag gaatttgccc ttgttgtcga acacgatcag cggatcggcg tgcgtgcctc
     3361 gctggaatac aaaaacctcg ttgcccgcct ggttggttgc tatagcggag actcgcccga
     3421 atttccagcc atcgggcatc gtatcgggct cttctccgaa acgaatgtgc acacggtact
     3481 tcaggagcga gccctggtgc ggataccagg cctcgggaac ccagccctgc tcgggcgcct
     3541 tggattttag ctgctgagcc caacaaactc caaaaaagag agcggctgcg atgatcagca
     3601 gtcctatatt cagctttttc atggatcaat gacctcccgg tttaaaaaga gcaagctcac
     3661 ggataatcag atctggttcg gcggaaccac gagcgctttc ttgtccgccc cgttgccttg
     3721 ctttgcggtc gaagtatatc gaacggaatc gcgaagtgtc aacgttccgg cctgcctggg
     3781 ccagcgtatt ggcgggattt cggccggtag acggcggcca agcgaccgct ggaggcgctg
     3841 ccgtggaacg gaattcccgg cgcgcactcc gcaactaccc ggagcaggtg cgccgctccg
     3901 gcgccaagca tactacgaat cgacaagccg gcttcttgac agaaagttag gtcagaaggc
     3961 ggtggcgacg acttcccttt cgacaacctc cgaacgccaa atcgcaacgc gcttccggtc
     4021 cgcgca
//
LOCUS       DASREK010000182        11877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_584506, whole genome shotgun sequence.
ACCESSION   DASREK010000182 DASREK010000000
VERSION     DASREK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584506"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VFA54_09340"
     CDS             <1..893
                     /locus_tag="VFA54_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS51051.1"
                     /translation="RSLHYSEKLERGAEEKRLHATRSEERDDLDRTIARALVLQARAW
                     GNTGDLDKAIAKAKQSYQTYPTGEAAREQARWEARQGKLADAIEHYADAFSIPDPKVT
                     AEDRRLDRAAAGDLYTKMHGSEAGLGDLMLQAYDRTAHAIAVRDALRKADVPNADVQD
                     AFLYRLSGLRGDSIRLADFSGKVLILDFWATWCGPCRLQHPLFEQLKSKYAKDADVVF
                     LSVDTDENRELVKPFIIEQKWSDQVYFEDGLVRFYHITGIPTTMIFNKRGELVSRMQG
                     VSFDRFVEQISEKIEEARRE"
     gene            1314..2573
                     /locus_tag="VFA54_09345"
     CDS             1314..2573
                     /locus_tag="VFA54_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263149091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1494..1496,aa:Sec)
                     /transl_table=11
                     /product="Trx7/PDZ domain-containing (seleno)protein"
                     /protein_id="HZS51052.1"
                     /translation="MRKRERYFALAVAAAGLLWSAAGRAVAQEDRIFWFSDYKEALQE
                     AKKTQKPIFLEFRCEAUIAGRIFDAQVVLSPPSSPRGKLMSQYVCVRLVKNDDSDIAL
                     FDRDWNNTLYFFIMNADEQIYMRYGGRDQVSDDTYLNLSSLEVALRQGLELHQKYLQG
                     ELKKTERPKPSHPRDYPLLVERTIGRGACVECHLIGDYINLHKERDGTLDKMKDLYRS
                     PDIKTIGIHLDIPKGLVVKEALGAVQEAGMRPGDRIAALNGTPVWTFGDFQYYYDKVD
                     RRAQQVQITVERDGKPIDLTVALPKLWWLTDIGWRQSSVDPRVYFESRPLTEAEKREL
                     GLKADGFAGQVKSVDSLAKMLGTHQLQVGDIIYGVDGVEQDEIANTPELFIKLRKTAG
                     QTVTLSVIRDGKRQQMQLNTLRMNFRK"
     gene            2577..3134
                     /locus_tag="VFA54_09350"
     CDS             2577..3134
                     /locus_tag="VFA54_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51053.1"
                     /translation="MSRTRRALKAVVTLVALSSPAWAGEWTQPVAVTHDMRPCISYRA
                     KLDGDFLVIQATPEAGWHTFAMDNKQRAEEKLAGKPALAVDAPTQIKLTQGLEAVGPW
                     YQSSPKDFSKPELQLFSWGFTGPALFAAKVRRSGAGPAQISIDGQACTETICKKVDVV
                     ISLPLTETKAGSPAGVDLKNLVRVR"
     gene            complement(3292..4068)
                     /locus_tag="VFA54_09355"
     CDS             complement(3292..4068)
                     /locus_tag="VFA54_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51054.1"
                     /translation="MITSLKFPNQTPVARPAQRGQKPSSGFWALFEQAPRKALAAPQA
                     ATTPERGGQAPQSKPAPAPIAPPQLSTLRNDRPQSVGVLPAAPQRGPNQAGAGLPALS
                     SFGKAAGVARETQEDPFSGTQTAASLVVYQPETGKENSIEGGAPGAEAPNNGPDSPPV
                     TDFIPNVPGMDLSHYALLIPPVSLQPLLDEMRRQALDPNQFQFTELQSYDAFPTRPDW
                     SVTMRQLLIQGPNGAKLYDLDWSLRTPWVTTVDVKSSHIA"
     gene            complement(4207..6576)
                     /locus_tag="VFA54_09360"
     CDS             complement(4207..6576)
                     /locus_tag="VFA54_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HZS51055.1"
                     /translation="MDAHSILEHIGRFPHGRANYKQLIRELGVRGDQRRELERLLAGL
                     VERGRLIETRSGHYMLAERQGEYVSGRLVLHRDGYGFVIPDQPERLARRGAEPSGVIP
                     DIYISAESAAGAMHGDRVLVRLKGPSEGRLVRILSRAHAEVVGQFHYSRRGSYVVPYD
                     EKIRENILIPRSAELPPPSKRMDRLGEVRWFEITSPEDLDGAVVNVEITGYPSPAEPA
                     QGRVTEVLGQPGDFGIDVEIMIRKHHLPHRFPPEVIREAEALEPVIAAEEIARRRDFR
                     ELDVVTIDGETARDFDDAVWVERRPDGRFQLQVHIADVAHYVREGSAIDREARLRGTS
                     AYFPDRAVPMLPYELSTGICSLNPRSDRLVLSVLLEIDPEGEVVDAEFCEGVIASAER
                     MTYTDVNLILTGDPGLRERYARLAPRFEQMAELAEILNRRRQKRGSIDFDLPEPVIEF
                     GEAGQVTAIVRSERNIAHRLIEEFMLAANEAVASTLELHLPASLYRVHEKPDPRRVME
                     FEQVAATFGYSLGIGPLPARDLGFTTRHRDHTKGRRSIIVPESDIPITSRHYQKLIAR
                     IAGKPEERILSYLMLRSLKQARYSEENLGHFALASASYTHFTSPIRRYPDLVVHRLLK
                     GLLRNFQGTLPKPEGTVQKSRRSRSGRSAAQNAARVSQVSKSYLHAVAEECSFTERRA
                     ADAERELLDWKKARFMEEHLGDEFPAIVVSVAKYGLFVELLDLFIEGLIPIETLPGDR
                     YTYRENMRAIVGERRKRKFTLGDRLTVRVDRVLAAERKVQFSWAPEPGK"
     gene            6730..8460
                     /locus_tag="VFA54_09365"
     CDS             6730..8460
                     /locus_tag="VFA54_09365"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HZS51056.1"
                     /translation="MRGFPMPVCLIAALCTALVGGPALAGDLHLRFARVTLAEGRVEI
                     DQTGTGEKIPAARNLPVGQGFWVETFGGAQAELEMDDGSYLRLGPDSQAELSDLAGLS
                     TGQRISRISLERGVLYATAEPIRPNVFAVAAPGVEIAFLTASRVRVEAGEKGSTVVIL
                     EGRVRLRTQAAELDLHEGETAHVDPQVGDRFQLLREAQGGVLDAWNEQRDREAAQRAA
                     GTKIKVFPAGLGELDAAGKWIDAGPLGKVWRPNVATGWKPFSLGLWRWYDGLGYVWVS
                     SESWGWLPYHYGRWTRLPSAGWVWAPGDDPAFKAGDTYWLRSAAFVGWGPLAPGENWR
                     PGVPPALFSPANTTFALYAPGSSGAPHVIDPQPAPSLPPDSLKLSRFVGALPAPLRPV
                     TSSIHRKRSLAGVASFAPAQVGTAARDAEMPHAGAGIQIIGGEPPRPPEAPQPPAAPS
                     ARYYEYPNGPPAGGVSQAPPGSSPGLTLQDDTEDDLGIYWPGLIVIQGGAGRSRSGMI
                     RNGSRGASGNGRPAGGGAPAPAPPSNPATPPAPNLTPPKVAAPTGPPGPERSMRYRIP
                     ESGEAESRRR"
     gene            8474..9241
                     /gene="rlmB"
                     /locus_tag="VFA54_09370"
     CDS             8474..9241
                     /gene="rlmB"
                     /locus_tag="VFA54_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZS51057.1"
                     /translation="MLKLIGIHAVQAALKAGRPVEYVMIARGAQNARIQQIIDACRRS
                     AVPLRFEPRETLDRAAEVKSHQGVVALAAAYPYREIEEVIAPPGTPGVLVLLDGVEDP
                     HNLGAIIRSAYAAGANAVVIPERRSTGLTETVAKAAAGALEYLPVARVPNINRALEYL
                     KEKDYWIYGLDERAEKSYNHADYRGRIAFVLGGEAKGLHQQTARHCDFLIKIPVAGRI
                     ASLNVSVAAAVVLFEAFRQRSAESGGETAGHSPRRRQ"
     gene            complement(9327..9713)
                     /locus_tag="VFA54_09375"
     CDS             complement(9327..9713)
                     /locus_tag="VFA54_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51058.1"
                     /translation="MMIPLPFTHLPWMPIGVAALAAFALGVLYRWASALRRDILALEL
                     EHGLMKKRFEAATGGLHVRLLQVERRLRPIAAAVSLQTGVSAADLRASAGAGEARILP
                     PLGAKNQLSRGEMELLKKIRQLASHS"
     gene            complement(9824..10765)
                     /gene="fliN"
                     /locus_tag="VFA54_09380"
     CDS             complement(9824..10765)
                     /gene="fliN"
                     /locus_tag="VFA54_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HZS51059.1"
                     /translation="MNIEARPRAIEGRVAGESEEEVEGRDSQFWEAVGRGRWLAEAWK
                     DGLRRVLESMGAARSGEEPEEDIQFEVGASEAENSSVWVSPTGCLFTLEGVPGAALWA
                     GAPSITVAALGEMISGCASLNESDLRDAFLDLMKQTAPEAGEILSGRLRRRISFSPGQ
                     IAGLPAQPGCSFQITFSCKNSPHAIGLALSPELLEYLDSPTETGLSAAPRSHSPATEG
                     EHQGAGGILPRNLDLLMEVELPVCVTFGRTQLPLKDVLKLSSGSIVELNRLANEPVEV
                     LINNYVIARGEVVVVDGNYGIRVTEIVSRQERIRSIF"
     gene            complement(10769..11737)
                     /gene="fliM"
                     /locus_tag="VFA54_09385"
     CDS             complement(10769..11737)
                     /gene="fliM"
                     /locus_tag="VFA54_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HZS51060.1"
                     /translation="MSFETQLSQEEIDRLFREAGTTVPEESKAAGKKSFSYDFRRPDR
                     IPKEQLRSIHLLHDFFARNLASSLGAYLRAYVVVHLVSVEQLSFAEFLQYLPTPTCIS
                     TIGMKPLEGNAVLELNPSLVFPMLDILLGGTGKNAMKEFREITEIEQSIIEGVLRIVL
                     HDLTETWRPVSEINFSIESTETQPQLIQILSPNEAIVAIGFEITTGENRGMMNFGIPS
                     IMVKMLGQQFEQQWSIRRKTVNEEEQRVMQRIVNGLPMTLDARLEGGAIRTADLLQLE
                     AGDVLTFDIPLSRPVSVGVNGKSKFQGEIIVIGKNRAIVIGGHGEH"
BASE COUNT         2360 a         3629 c         3609 g         2279 t
ORIGIN      
        1 ggcggtccct gcattactcg gagaagctcg agaggggcgc cgaagaaaag cgtctccacg
       61 ccacgcgcag cgaagaacgc gatgacctcg accgcactat cgcgcgagcc ctggttcttc
      121 aggccagggc gtggggcaac accggcgatc tcgacaaggc catcgccaag gcgaagcaga
      181 gctaccagac gtatcctacg ggcgaagcag cgcgggaaca ggcgcgatgg gaggcccggc
      241 aaggtaagct cgccgacgca attgagcatt acgcggatgc tttttccatc cccgacccca
      301 aggttacggc cgaagaccgg cggctggacc gcgctgcggc gggcgatctc tatacaaaaa
      361 tgcacggatc ggaggcgggc ctgggagatc tgatgttgca ggcttacgac cgcacggcgc
      421 atgccatcgc agtcagggat gcgctccgga aggccgatgt acccaatgcc gatgtccagg
      481 atgcattcct gtaccgcttg agcgggctgc gcggcgactc tatccggctg gccgatttca
      541 gcggcaaggt tttaatcctc gatttctggg ccacctggtg cggcccatgc cgtctccagc
      601 acccgctttt tgagcaattg aagagcaagt acgcaaaaga cgccgacgtg gtctttcttt
      661 ccgtcgacac tgatgaaaat cgggagctgg tgaagccttt catcatcgaa cagaaatggt
      721 ccgaccaggt ttatttcgaa gacggcctgg tgcgctttta tcacatcacg ggtattccca
      781 ccaccatgat cttcaacaag cgtggagagc tggtcagccg gatgcagggc gtcagcttcg
      841 accgtttcgt cgagcagatc agcgagaaaa tcgaggaagc gcggcgggaa tgagcgcgcc
      901 cggttccggg tccgcggtcg cgtgggattt cggaagccgg cgttttcttg cgattctcgc
      961 ctctcaaacc cgtcctgcgt gaagggccct ggttcctcaa gctgtcttct ccaagcaggg
     1021 tctcgagcgg gaaaccgctt ctacgctcgc cggttctccg agggcctgca aatcccggga
     1081 tggcggccgc gccccacttc agggccggac cggcccgctg gcaggaaagc cgatgctata
     1141 atccaaacgt ctggctcgtc cgggtcatcc tgcaaggggc tgatcccgcc cttcggcagg
     1201 acggactggg aaagaaattt ctgagtccgg actggatcgg aaatcatcta tcgagaaacc
     1261 gccggggtgt gctctgtgcg aagcggccgc tctttcggcg gatgtggatg ctcatgagaa
     1321 agcgagaacg atacttcgct cttgcggttg cggcagcagg cttgttatgg tccgccgctg
     1381 gccgggcggt cgcgcaggaa gaccgcattt tctggttcag cgattataaa gaggccttac
     1441 aggaggcgaa gaaaactcaa aagccgattt ttcttgagtt ccgttgcgag gcttgaatag
     1501 ccgggcgaat atttgacgca caggttgtgc tgtcgccccc aagctcacca cgcggcaaac
     1561 tcatgagcca gtacgtgtgt gtccggctcg tgaagaacga cgattccgat atcgcgctgt
     1621 ttgaccggga ctggaacaac acgctgtact tcttcatcat gaacgccgat gagcagatct
     1681 acatgcggta cggcggccgg gaccaggttt ccgacgacac ctacctcaat ctgagcagcc
     1741 tggaggtggc gctccggcag ggcctcgaat tgcatcaaaa gtacctgcag ggcgaactga
     1801 agaaaacgga gcggcccaag ccttcgcatc cgcgggatta cccgctgcta gtcgagcgca
     1861 cgatcgggcg gggcgcctgc gtcgaatgcc atctgatcgg cgactacatc aatctgcaca
     1921 aagagcgcga cggcacgctc gacaagatga aagatcttta ccgctcgccc gatatcaaga
     1981 ccatcgggat ccatctcgat attcccaaag gattggtcgt gaaagaggcg ctgggcgcgg
     2041 ttcaggaggc gggcatgagg ccgggcgacc gcattgcggc gctgaacggc actccggtct
     2101 ggaccttcgg agatttccag tattattacg acaaagtgga tcgccgcgcc cagcaagtcc
     2161 aaattacggt ggaacgggat gggaagccga tcgacctgac ggtggcgctg ccgaagcttt
     2221 ggtggctcac ggatatcggc tggcggcaat ccagcgttga tccacgcgtc tatttcgagt
     2281 cgcgcccatt gacggaggcc gagaagcgtg agctagggct gaaggcagac gggtttgccg
     2341 gccaggtgaa atccgtggat agcctggcca agatgctcgg gacccatcaa ttgcaggtgg
     2401 gcgatatcat ttatggcgtc gatggcgtgg aacaggatga gatcgccaat accccggagc
     2461 tcttcatcaa gcttcgcaaa acagcgggcc agaccgtgac cctgagcgtg attcgggacg
     2521 gcaagcggca acaaatgcaa ttgaacacgc tcaggatgaa cttccggaaa taagctatgt
     2581 cgagaacacg aagagcgctg aaagcagtcg ttaccctggt tgccctcagc agcccggctt
     2641 gggctggcga gtggacccaa ccggtagccg tcactcatga tatgaggccg tgcatttcct
     2701 acagagccaa actcgacggc gacttcctgg tgatccaggc aacgccggaa gccgggtggc
     2761 atacgttcgc catggacaac aaacaacgcg ccgaggaaaa gctggccgga aagccggcgc
     2821 tggccgtgga cgcccccacg caaatcaaac tcactcaggg attagaagcc gtggggccgt
     2881 ggtatcaatc ctcgccaaag gatttttcaa agccggagct ccagttgttc agttggggct
     2941 tcaccggccc ggctctgttt gcggcaaaag taaggcgctc cggagcggga ccggctcaaa
     3001 tctcgataga cggacaggcg tgcaccgaaa cgatttgcaa gaaagtggat gtggtgattt
     3061 cgctgccgct caccgagaca aaagccggca gcccggcagg tgttgatctg aaaaacctcg
     3121 tgcgcgttcg ataggcgcct gccgcggctc ccaccggcta agaaacgagc gttctcgtcc
     3181 agtcaccgcg tccgcttcca gtacactggc gcctgactag cggcgccgct tcccactcgc
     3241 ctcgggacgg tcagcgtgga aagagaggca gagagccgac ccggcctgac ttcaggcgat
     3301 atgggagctc ttcacgtcaa ccgtagtcac ccaaggcgtc ctgagagacc agtcaaggtc
     3361 gtacagcttg gcgccgttgg gaccctgaat caaaagctgg cgcatggtta cgctccaatc
     3421 gggccgcgtg gggaaagcgt cataactttg cagttcggtg aactgaaact gattggggtc
     3481 aagggcctgt cggcgcatct cgtccagcag gggctggagc gacaccggcg ggatcagcag
     3541 cgcgtaatgg ctaagatcca tacccggcac attggggatg aaatcggtga caggcggaga
     3601 atccggcccg ttattcgggg cttcggcgcc gggggctcca ccttctatag aattctcctt
     3661 gccggtttcc ggctgataaa ccacaaggct ggcagccgtt tgggtcccag agaagggatc
     3721 ttcctgtgtc tcccgcgcaa cgcccgccgc ttttccaaag ctggatagag caggcagccc
     3781 ggcgccggcc tggttcgggc ctcgctgagg tgcggccggc agcacgccaa cggattgggg
     3841 acggtcattg cgcagcgtcg aaagctgggg tggggcgatg ggcgccggag ccggcttgga
     3901 ttgcggcgcc tgcccgcccc tttccggtgt tgtagccgcc tgcggcgcgg caagtgcttt
     3961 tcgcggcgcc tgctcaaaga gcgcccaaaa gcccgagctc ggtttttggc cccgttgcgc
     4021 cggccgtgcc accggagtct ggttcggaaa cttcagggat gtaatcatgc ggcctcccgc
     4081 cgggatgttc ggcaactatg ccgccaaacg gcaaatgggg ctgtttgcga agcttgagcg
     4141 gtacggccag aagattggcg ggacaggatc ctggcacaac ggcaattttc tggcgccgcc
     4201 ggcggctcat ttgcctggct cgggcgccca ggaaaactgc accttgcgtt cggcagccag
     4261 cacgcgatcc acgcggaccg tcagccggtc gcccaatgtg aactttcttt tgcgcctctc
     4321 cccgacaatc gcgcgcatat tttcgcgata agtgtagcgg tctcccggca gggtttcgat
     4381 ggggatgagg ccctcaatga acaaatcgag caattccaca aacaggccgt acttggcgac
     4441 gctgacgaca atcgcgggga attcgtcgcc taggtgctct tccataaatc gcgccttttt
     4501 ccagtccagc agttcgcgct cggcgtctgc cgcccgccgc tcggtaaagg agcactcttc
     4561 ggcaacggcg tggagataac ttttggaaac ctgcgaaact ctggcggcat tttgcgccgc
     4621 gctcctgccg cttcgcgagc gcctggattt ctgcacggtt ccttccggct tcggcagggt
     4681 tccctggaaa tttctcagca agcctttcaa aagccggtgc acaaccagat cgggatagcg
     4741 gcggatcggc gaagtgaaat gggtataaga agccgaggcg agggcaaaat gacccaaatt
     4801 ctcctcgctg taccgcgctt gcttgagcga tcgcagcatc aggtagctta ggatgcgctc
     4861 ctcgggcttt ccggcgatgc gagcaatcag cttttggtaa tgcctcgagg tgatgggaat
     4921 atcgctttcc ggaacaataa tgctgcggcg gccttttgta tgatcgcgat gccgggttgt
     4981 gaaacccaag tcgcgggcgg gcaacggacc aataccgagt gaataaccga acgtggccgc
     5041 tacctgctcg aattccatca cgcgccgtgg gtccggcttc tcatggaccc ggtacagcga
     5101 agccggcaga tgcagctcca gggtggaggc gactgcttca ttagccgcca gcatgaattc
     5161 ttcaatcaag cggtgcgcga tgttgcgctc gctgcgaacg atggccgtca cctgcccggc
     5221 ttccccaaac tcgatgacgg gctccggcag atcgaaatcg atcgacccgc gcttctgccg
     5281 ccgccgattc aggatttccg ccagttcagc catctgctcg aagcgcggcg ccagccgggc
     5341 gtagcgctcc cgcagtccgg ggtcgccggt aagaatcagg ttgacatcgg tatacgtcat
     5401 ccgctcggcg ctcgcaatca cgccttcgca aaattccgcg tcgaccacct cgccttccgg
     5461 atcgatttct agcaacaccg agagcacaag ccgatcggag cggggattca gcgagcagat
     5521 gccggtcgaa agctcgtacg gaagcatagg cacggcgcgg tccgggaaat aagcgctggt
     5581 gccgcgcaga cgggcctccc ggtcgatggc cgagccttca cggacatagt gcgccacatc
     5641 cgcaatgtgc acctggagct gaaagcggcc atccggccgg cgttccaccc acacggcgtc
     5701 gtcaaagtcg cgagccgtct caccgtcaat cgtgacgaca tcgagctccc gaaaatcacg
     5761 gcggcgagcg atttcctccg ctgcaatcac cggctccaga gcttccgcct cgcggattac
     5821 ctcgggcggg aaccggtgcg gcaggtgatg cttgcggatc atgatctcga cgtcgatgcc
     5881 gaaatcgccc ggctgtccca gcacttccgt aacccggccc tgagccggct cggccgggct
     5941 cgggtaaccg gtgatctcga cattgacaac agcgccgtcc agatcttccg ggcttgttat
     6001 ctcaaaccag cggacttcgc cgagccggtc catgcgcttc gatgggggcg gcaattccgc
     6061 actgcggggg atcagtatgt tttcgcggat cttctcgtca tacggaacta cataactgcc
     6121 gcgccggctg taatgaaatt gccccacgac ctcggcgtgc gcgcgggaaa ggatgcgcac
     6181 gagccgtccc tcgctcggcc ctttcagccg gaccagcacc cggtctccgt gcatcgcgcc
     6241 ggccgccgat tcggcgctga tgtagatatc cgggatcact ccggatggct ctgccccgcg
     6301 ccgggctagc ctctccggct ggtctggaat cacgaaacca tagccatccc ggtgcaaaac
     6361 tagcctcccg gaaacgtatt ccccttgtct ttccgccagc atgtagtggc cggagcgcgt
     6421 ctcgatcagg cgtccgcgtt ccacaaggcc ggccagcagc cgctcgagct cgcggcgctg
     6481 gtctccgcga acacccagct cccgaatcaa ctgtttgtaa ttggcccgcc catgcggaaa
     6541 gcgcccgatg tgctcgagaa tggagtgggc atccatattt cccctttgat cttaactgac
     6601 tccccagggg cccatggttc ccgttgcctg ccagcggaac cacctggaat cagccttcca
     6661 ggcacatccc cgccggttca agcctccgca ggatcgatat gctaaggtct aagtagcaga
     6721 agggagccca tgcgcggatt tcctatgccc gtgtgcctga ttgcagccct ttgcactgcg
     6781 ctcgtaggcg gtcccgcttt ggccggagat ctccacctgc gttttgcccg cgtcacactg
     6841 gcggaaggaa gagtggaaat tgaccagacc ggaaccggcg aaaaaattcc tgccgcgcgc
     6901 aacctgcctg tgggtcaagg attctgggtc gaaaccttcg ggggcgcaca ggctgaactg
     6961 gaaatggatg atggcagcta cttgcgcctg gggccggact cccaggcgga attatccgat
     7021 ttggccggac tgtctacagg acagcgcatc agccgcattt ccctcgaacg cggcgtgctt
     7081 tacgccaccg ccgagccgat tcgtcccaat gtctttgccg tagccgctcc tggagtggaa
     7141 atcgcgtttc tcaccgcatc ccgcgttcgc gtggaagcgg gcgaaaaagg atccaccgtg
     7201 gtaatcctgg agggccgcgt ccggctccgc acgcaggcgg cggaactaga tctccatgaa
     7261 ggggaaacgg cccatgtcga tccgcaagtg ggggatcggt ttcaactact gcgcgaagcc
     7321 caaggcggcg tcttggatgc ctggaacgag caacgcgatc gggaagcggc ccagcgcgcg
     7381 gcaggaacta agatcaaggt tttcccagcc gggttaggcg aactcgacgc tgccggtaaa
     7441 tggatagacg ctggccctct tggaaaggtg tggcggccca atgtcgcgac aggctggaaa
     7501 ccgttttctc tcgggctctg gcgctggtat gacggtctcg gctatgtgtg ggtatcgagt
     7561 gaaagctggg gctggctgcc ctatcactac ggccggtgga cccgcctgcc gtcggccggt
     7621 tgggtgtggg cgccgggcga tgaccctgcc ttcaaggcag gtgatacgta ttggctgcgc
     7681 tcggccgcgt ttgtgggatg gggcccgctg gcgcccggcg aaaattggcg gccgggcgtc
     7741 ccgcccgccc tcttctcccc ggcgaacaca acattcgctc tttacgcgcc cgggagcagc
     7801 ggggcgccgc acgtcatcga tccgcagccg gcgccgtctc tgcctccaga ttccctcaag
     7861 ctgagccgtt tcgtaggagc ccttcctgct ccgctgcggc cggtgacctc atcgattcac
     7921 cgcaagcgga gcctggcggg cgtagcgagc tttgccccgg cgcaggtggg aacggccgct
     7981 cgcgatgcgg aaatgcctca cgccggcgcc ggcattcaga ttattggcgg cgaaccgccg
     8041 aggccgcccg aggcgccgca acctcctgct gccccttctg ctagatatta tgaatatcca
     8101 aatggccctc ccgcgggagg agtttcccag gcgccgccgg gttcctctcc gggacttact
     8161 cttcaggacg acacagaaga tgacctgggc atttactggc ctgggctaat cgtcattcaa
     8221 gggggcgccg ggagaagcag gagcggaatg attcgtaacg gctctagggg agcttcgggt
     8281 aatggccgcc ctgccggggg cggcgccccg gctccggccc cgccatccaa tccggcgacg
     8341 cctcctgcgc ctaatttaac gccgccgaaa gtagccgcgc caaccggccc accgggaccg
     8401 gagcgctcga tgcgctacag gattcccgag tcgggcgagg cggaatcgcg aaggcgttga
     8461 ccgccgcttc tccatgctca agttgatcgg gatccatgcc gtgcaggcag cattgaaggc
     8521 cgggcgcccg gtggagtacg tgatgattgc gcgcggggcg caaaatgcgc ggatccagca
     8581 aatcatcgac gcttgccgcc ggtcggcggt tccgctccgc ttcgagccaa gagagacgct
     8641 cgatcgcgcg gccgaggtga aatctcacca gggcgtagtg gcgctcgccg ctgcgtatcc
     8701 gtaccgggag atcgaagagg taattgcgcc tcccggaaca ccgggtgtcc tggtgctcct
     8761 ggacggtgtg gaggaccccc ataatttagg cgctatcatt cgctccgcct atgcggccgg
     8821 agcgaatgcc gtggtcatcc cggagaggcg ctcaaccggc cttacggaaa ccgtcgccaa
     8881 ggcggccgcc ggcgctctcg aatatcttcc ggtggctcgc gtgccgaaca taaatcgggc
     8941 gctggaatac ctcaaagaaa aggattattg gatttacggt ctcgatgaac gcgcagagaa
     9001 gagctacaac cacgccgact atcgcgggcg gattgcgttc gttctcggag gcgaggcgaa
     9061 gggtctgcac cagcaaacgg ctcggcactg cgactttcta atcaaaattc ctgtcgcagg
     9121 gaggattgca tctctcaatg tctccgtggc cgctgccgta gtgctattcg aggctttccg
     9181 gcagcgcagc gcagaaagcg ggggcgaaac tgccgggcac tcaccccgtc ggcgccaata
     9241 agctgcgcct tccgcgctaa gcggaccgtc ctgtgccgag ccgcacgccg ggcgatctgc
     9301 gggaaacgat tacgcgcgcc cggaagctaa ctgtgggaag caagctggcg gatctttttc
     9361 agcaactcca tttcaccgcg ggagagctgg ttttttgctc ccaggggcgg aagaatcctt
     9421 gcctcccccg cgccggcgga tgcgcggagg tctgccgcgg atacgcccgt ttgcagggat
     9481 acggccgctg caatcggccg caggcgccgc tctacttgca acagccgcac gtgtaacccc
     9541 ccggtggccg cctcgaatct ctttttcatc agcccatgtt ccaattcgag cgccaggata
     9601 tcccgccgca aagcgctcgc ccagcggtat agcacgccga gcgcaaacgc cgccagcgct
     9661 gcaacgccga tcggcatcca cggcaaatga gtaaaaggca gaggaatcat cataaaaaag
     9721 tcctgaggcg cctgtttctc tcgttactgt cgggatcggg agcgggcttc tcgccgcgcc
     9781 ataaccggcg ccttagccgt caaaggcggc gccgccggaa ggcttaaaat atggatcgaa
     9841 tccgctcctg ccggctcacg atttcggtca cacggatgcc atagtttccg tcaacgacaa
     9901 caacctcgcc ccgcgcgatc acataattgt tgattaacac ctcaaccggc tcattcgcca
     9961 ggcggtttaa ctcgacgatc gagccggaag aaagcttcaa cacatccttt agcggcaatt
    10021 gtgtgcggcc gaaggtgacg cacaccggca actcgacctc cattagcaga tcgagattac
    10081 gcggcagtat tccgcctgcg ccctggtgct ctccttctgt tgcaggagaa tgggagcgcg
    10141 gtgcggccga aagaccggtc tccgtcgggc tgtccaggta ctcgagcagc tctggactca
    10201 acgcaagccc aatcgcatgg ggagagttct tgcaggaaaa agtgatttgg aatgagcagc
    10261 ccggctgagc gggaaggccg gcgatctgtc cgggggaaaa gctgatgcgc ctgcgaagcc
    10321 tgccggagag gatttcaccc gcctccggcg cggtctgctt catcagatcc agaaacgcat
    10381 cgcgcagatc cgattcattt aaggaagcgc agcctgaaat catctctccg agcgcagcca
    10441 ctgtaatgct gggagcgcct gcccagagcg cggcgcccgg aacgccctcc agcgtgaaga
    10501 gacacccggt tggcgacacc cagacggacg agttttccgc ctcagaggcg ccaacctcga
    10561 attgaatatc ttcttccggt tcttcccccg aacgggccgc tcccatcgat tccagaacgc
    10621 ggcgcagccc gtctttccag gcctccgcaa gccaacggcc ccggccgacc gcttcccaga
    10681 attggctgtc tcgaccttcc acctcttctt cactctcccc tgcaacccga ccctctattg
    10741 cgcggggacg cgcctcaatg ttcatacctc aatgttcccc atggccgccg atcacaatgg
    10801 cccgattctt gccaatgact atgatctctc cttggaattt actcttcccg ttcactccga
    10861 ccgaaaccgg gcggctcaac ggaatatcga aggtcaacac atctcccgct tcgagttgca
    10921 ggaggtcggc tgtgcggatg gcgcctccct ccaggcgggc gtccaaggtc atcggcagcc
    10981 cattgacgat tcgctgcatc acccgctgct cctcttcatt caccgttttg cggcggatcg
    11041 accactgctg ttcgaattgc tgcccgagca ttttgaccat gatcgaagga atgccgaaat
    11101 tcatcatgcc gcggttttcg ccggtggtga tctcaaagcc tatcgccaca atcgcctcgt
    11161 taggagagag aatttgaatc agttgaggct gcgtctctgt ggactcgatt gagaaattga
    11221 tctccgacac gggacgccag gtttcggtaa gatcgtgcag cacaatgcgc agcacgcctt
    11281 cgatgatgct ctgttcgatt tcggtgatct cccggaactc cttcatggcg ttcttgccgg
    11341 tgccgcccaa cagaatgtcg agcataggaa aaaccagcga ggggttcagc tccagcaccg
    11401 cgttgccttc gagcggcttc atgccgatcg ttgagatgca ggtcggcgta gggagatatt
    11461 gcagaaactc ggcaaaggac aattgctcga ccgacaccag gtgcaccacc acgtaggcgc
    11521 gcagataggc gcccaaggaa ctggccaggt tgcgggcgaa gaaatcatgc agcaggtgga
    11581 tcgatcgaag ctgctccttg ggaatgcggt ccggccgccg gaaatcatag ctgaacgact
    11641 ttttgcccgc cgccttgctc tcttcaggca cggtcgtgcc ggcttcacgg aataggcgat
    11701 cgatttcttc ctgcgagagt tgtgtttcaa atgacatacg gattcaccat ttggttaagt
    11761 cggccatctc gatcgcaaat acgttcgcag ccggagaatg gcctgcgatt tcagttgcga
    11821 gatgcgggtt tcatgcatct tcaagacctc ggcgatttgc cggagcatca gctcctc
//
LOCUS       DASREK010000183        21853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2233849, whole genome shotgun sequence.
ACCESSION   DASREK010000183 DASREK010000000
VERSION     DASREK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 21853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21853
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2233849"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..788)
                     /locus_tag="VFA54_09390"
     CDS             complement(531..788)
                     /locus_tag="VFA54_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51061.1"
                     /translation="MTGDRSDLQEAWAQALAEYSRQVEKSLELLRFSGARPTAEEWAA
                     IERQRLVERSAFEKYRKARRAYLEFLSGAGSLALMLAFHLR"
     gene            complement(808..1077)
                     /locus_tag="VFA54_09395"
     CDS             complement(808..1077)
                     /locus_tag="VFA54_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51062.1"
                     /translation="MVDRNNNKKTPTCSECSRLWRAYLEATQSQLELMHSMPSRNTDF
                     DAIQELEGRIELAAARRERTWQEIQEHEESVHGRHDAGVSIDKGE"
     gene            complement(1087..1209)
                     /locus_tag="VFA54_09400"
     CDS             complement(1087..1209)
                     /locus_tag="VFA54_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51063.1"
                     /translation="MTQMYLIVPWNKACTRQFEYRVAAIRWARLERKLAGGGEA"
     gene            complement(1453..2652)
                     /locus_tag="VFA54_09405"
     CDS             complement(1453..2652)
                     /locus_tag="VFA54_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZS51064.1"
                     /translation="MRSEFPFSSVSLFLIAVLLMQPACGNGKTENTDHSAHAAPVAGA
                     RRPSAAVAALAQAPPLPGMPPVLNPENIYAADSAGNLSPEVRNFRPLVYVPNSGSNTV
                     DVIDPKTYKIVDHFRVGRQPQHVTPSYDMKKLWVLNDLGDSVTEIDPATGKKGKTIRV
                     EDPYNMYYTPDGKYAIIVAERLHRLDFRDPQTMKLVHSTPVPCRGVDHMDFSANGRYL
                     VASCEFSAEVIAVDVAEQKVVAELPLAPGGMPQDVKLSPDGKIFYVADMAANVVYEID
                     SSNFTIAGSLPSGKGVHGLYVSRDGRLLYASNREEGSISVIDLASRKIVEKWRLPGGG
                     TPDMGGVSAEGKVLWLSGRYSREVYAIDTSDGHLLARIRVGKGPHGLCVYPQPGRYSL
                     GHTGVFR"
     gene            2804..3193
                     /locus_tag="VFA54_09410"
     CDS             2804..3193
                     /locus_tag="VFA54_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HZS51065.1"
                     /translation="MNYYAVLGVDADADEKTIRSAFRALARRYHPDAGEGSSEAKFRQ
                     VLQAYEVLSNPVRRREYDQSQAALKTRPPFSFGEVRRPAGVSPAAPLRQRAWDPFLEA
                     RTSIFEMMQVFERLWRWDEDFFGEPFF"
     gene            complement(3226..3594)
                     /locus_tag="VFA54_09415"
     CDS             complement(3226..3594)
                     /locus_tag="VFA54_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51066.1"
                     /translation="MRLVTEEEEGFGLERLPPGVYGFTYSPGIANSPLFRKRIYHSLE
                     VHKLPDSSVALIGFLPEKTAAKVEAGEEALDIRLFPEPDSEASQAVSIPLARVKKVSE
                     FSMREAGRLALQLYPARRER"
     gene            3902..4762
                     /locus_tag="VFA54_09420"
     CDS             3902..4762
                     /locus_tag="VFA54_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS51067.1"
                     /translation="MTSKRLAMCAALCAAVWSMPRMAWAQQPAGSVRMYNTAKQKLLS
                     GKQIFGGTATSPDPNIYCALANAGFDFTWIEMQHSPMTYEDVARMVWACRGSSAIPFI
                     RVPDATESDIQKATDLGALGIIVPTVDTVEKAQAAVKWAKYPPQGRRSQGAGHATAIW
                     GSDYRQTVNDNMMVVIMIETPTGVENAEKIASVPGIDVIFAASGDLGNFSGHFMDPKT
                     HKGDSYYESLVTRIHDVTLAHNIKLGGPDVWKDRPGYSFFQSSSEPGLIRAGAQALGF
                     KPVPYPAQRQ"
     gene            complement(5024..7624)
                     /gene="glnD"
                     /locus_tag="VFA54_09425"
     CDS             complement(5024..7624)
                     /gene="glnD"
                     /locus_tag="VFA54_09425"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZS51068.1"
                     /translation="MTAGAPGLLAGLEPIKQKFLETGDGLEMVRQRSDAVDRAVAALF
                     HEAFGEQAASGVALVAVGGYGRRQLFPCSDVDLLFLFERDSAAEAARDRIAKLLRGLW
                     DGGVRASHSVRTPAEAASLAPDNVELNISLLDARYLAGDMAALARLFERLPGFYAREK
                     QTLARGLDRLTRARHAKHGDTIYHLEPNVKEAPGGLRDFQVACWLSQLAHAEEAAANA
                     VPPEARGFALSPPEQHLPAETRDAWMEAKRFLFALRCYLHCFNGRDNNILTFENQERL
                     AERAGASPQDAAVWMRDYFRNVRVIHRLARRTLDEVATPEHSLFTMFRDRKSRLSNPD
                     FAVARGMLYMRHPEALVQNPGLAMRLFEFIARHGVPPAPETERRIGGAAGNFREYLRK
                     QCGASSGLWFSIASILRLPHAYQALVVMRETGLLAALFPEYERIDCLVVRDFYHRYTV
                     DEHSLRTIKTLHELRAGSDDALTQHFSELMQETERPEVLLFALLFHDVGKGGWEGNHT
                     TAGLALAEQAMERIGMPEPERETVRFLIAHHLDMPAIMTGRDLADPETARAFASQVGT
                     LERLQSLTLMTYADISAVNPEALTPWRKDLLWQLYVATHNQLTREMEAERIGTGAAVA
                     AQLASNYSERQALARFLEGLPTRYLRIHGPDQIRRHFQLKPVLERRSVAVQLGRRHSL
                     YELVVLARDRAFLLASIAGTISSFAMNIVKAEAFSNRDGMILDTFIFADPNRTLELNP
                     SEADRLTSTLEQVVLGKIDVRELLHKRRTPAATHRPRIARHIAFDNQTSPNETLFHVI
                     TEDRPGLLYDLASTFSFNRCDIDVVLIDTEGRKAIDVFYVRSKGRKLDDAAARRLCLK
                     LERACSGSEE"
     gene            complement(7621..7959)
                     /locus_tag="VFA54_09430"
     CDS             complement(7621..7959)
                     /locus_tag="VFA54_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZS51069.1"
                     /translation="MKKIEAVIQPHKLEDVREALKEVGIDGMTISDVRGHGRQKGHRE
                     VYRGQEYTVDLLPKVKIEIVVPDSRLEETVKALGDAARTGKLGDGKIFISSIDDAIRI
                     RNNDRGEGAL"
     gene            complement(8097..9515)
                     /locus_tag="VFA54_09435"
     CDS             complement(8097..9515)
                     /locus_tag="VFA54_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZS51070.1"
                     /translation="MKRPMRVVCALALLLALACSLGPAAAQTAAAGPDPASDRLAKME
                     TALKTAQSAGDNAWMLVSCALVLMMTGPGLALFYGGLVRTKNVLSTMMQSFFLMGLVT
                     VLWALVGYSLAFSEGNAFIGGLHYAFLHGVGLAPNADYAATVPHQTYMMFQLMFAIIT
                     PALITGAFAERIKFSAMVLFMILWSLAVYFPMAHMVWGKGGLLNASLGGKIPCFDFAG
                     GTVVHISSGVSALVCALYLGKRYGYPHEPMKPHSVVLSVIGASLLWVGWFGFNAGSAV
                     AASGLATSAFVNTHFAAAAAALGWSIGEWLKNGKPSVLGAISGAVAGLVAITPGSGFV
                     APMSALFIGLIAGLVCFLMVMVVKGKMGYDDSLDAFGVHGAGGTLGALLTGVFATNAI
                     NNTMQDPSGHSRALGLADGNAGQILNQLWGVLICWLLAALATFIILKIVDALLGVRVS
                     KEDEIEGLDLSQHGEEGYNFEG"
     gene            complement(9651..9956)
                     /locus_tag="VFA54_09440"
     CDS             complement(9651..9956)
                     /locus_tag="VFA54_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HZS51071.1"
                     /translation="MRVLSILLLFAALLCWSAPVFAQGPPADASHQWVVITAGFAMAI
                     ASGLCGLGQARATAAAAEGMARNPAAAAAIRFALILGLALIESLALYTLVIIFVKVS"
     gene            complement(10116..10856)
                     /gene="atpB"
                     /locus_tag="VFA54_09445"
     CDS             complement(10116..10856)
                     /gene="atpB"
                     /locus_tag="VFA54_09445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZS51072.1"
                     /translation="MEHELWFTKLLNGYLAGLANAILALFHLHAANPERPWRDFISMQ
                     ILVALIIIVLFALLRGRLTVERPGNFQQSFEVLYEFVAGQGEEIVGHGSHRYLAFFGT
                     LFIFILFANLLGIIPGFGSPTQYIPGPVPFGCALASFLYYNIEGVRAQGVGKYLAHFA
                     GPVWWLAWLMVPIEIVSHSARVMSLTIRLFANMFAGEMVTMIFIGLVPIAIPAIFMGL
                     HVFVSLLQAYIFMLLVMVYVQGAVAHEH"
     gene            complement(10846..11376)
                     /locus_tag="VFA54_09450"
     CDS             complement(10846..11376)
                     /locus_tag="VFA54_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51073.1"
                     /translation="MDADPAADQAASDFYAAAERRIWRFQLAFGVLGVGGAWRLAGAR
                     GALGFTMGAVISAVNFFWLKQAVDTLAARAAAADLGTRQAGTEQTRGRPGVADVQGLP
                     PIAGAGLARRTVRRVIRRFLGRYALIAAAAYATFRYTAWSVKALLAGLFLFVAAILAE
                     ICVEIAIGLKQDRHGT"
     gene            complement(11569..11760)
                     /locus_tag="VFA54_09455"
     CDS             complement(11569..11760)
                     /locus_tag="VFA54_09455"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZS51074.1"
                     /translation="MQVAEYTSLAFTLPACTAAGYLIGYGLDRLFHTSFLSIVFLILG
                     IAGGFIELIRQVQKDAGDH"
     gene            complement(11838..13313)
                     /locus_tag="VFA54_09460"
     CDS             complement(11838..13313)
                     /locus_tag="VFA54_09460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HZS51075.1"
                     /translation="MNFAFTQTDFFVLKPAILLCLFGCGILITDFFIDVRQKFFNAIT
                     ALIGLGFTAYAVWQQQSFLRANGLAELTGLSGAVTVDGFGIFFDWIFLIATALAVIIS
                     TRYLEIEQEHHGEYYALLLFAQTGMVFLATGAELVTLFIGLELMALSFYVMVGFLRGD
                     KRSNEAAMKYLLLGAFSSGLLAYGFSIFFGISGSTRLGDIARAIAGRDPRDPLVFLAM
                     ATTGVGLFFKIAAAPFHMWAPDAYEGAPTTVTAYLSVASKAASFAFLLRIFQVPLESM
                     RATWEPLVAAVALLTMTLGNLAAISQTNIKRLLAYSSISHAGYILLGLVSGNQTGLKG
                     IAVYILVYTFMNFGAFTIVVAMRNKNIIGDEIDDMSGLMFKHPTYAVLMLVFLLSLAG
                     IPPTAGFLGKYYIFLSLIQTQHYYLAVFAALYVAVALYYYFRIVKVMFVSEEAAAPVE
                     LSDSLGVRLALGVTAAFTVLIGVYPEPFLQMAQRSLFAALR"
     gene            complement(13310..14869)
                     /locus_tag="VFA54_09465"
     CDS             complement(13310..14869)
                     /locus_tag="VFA54_09465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZS51076.1"
                     /translation="MQQHYLSILLFTPLAGMFILLLIPKENKTAIRVWANLVLFLGFI
                     LSVPLWWMYDRSNADYQFVERANWIPSIGAHYALGIDGISLLLILLTTLVGFISVLSS
                     WSAIQDRVKEYYAFFLLLQTGMLGVFMALDFFLFYVFWEVMLVPMYFIIGVWGGPRKL
                     YAAIKFFLYTLFGSVLMLLGILTLYYHYYSQFGTYTFDIQELTRMSIPLTMQKWVFWA
                     FFLGFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFIRFSLPLLPDAAK
                     DGFTVKMIAALSIIGIIYGALVSLMQKDWKKLVAYSSVSHLGFCTLGIIALNPNGLTG
                     SVIQQINHGISTGMLFLIIGIIYERRHTREISEYGGLSNVMPVYAVIFVIAMLSSAGM
                     PLLNGFVGEFTILQGAFEVSKLWAAFAVSGIVLGAAYLLWLYQRTMFGPITNPKNEHL
                     KDLNAREIAYMLPLIAWAFWIGVYPKPYFEILEKPVAKIVKRVQPDYYRVRNLKDPLE
                     QPGQVAQIATP"
     gene            complement(14969..17095)
                     /gene="nuoL"
                     /locus_tag="VFA54_09470"
     CDS             complement(14969..17095)
                     /gene="nuoL"
                     /locus_tag="VFA54_09470"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HZS51077.1"
                     /translation="MVNGAETSFFLQHIWLIPLFPLFGAAAMLLFGRKLDPQPEWGRQ
                     HDALDHREHGHHESSGRSVIRILCPGMVLIAFLFSLKAVLDLAALPAAERHAEVLLAP
                     WITGVGAFQAPWGFLLDPLSSVMILVVTGIGFLIHVYSIGYMAHDGGFYRFFGYLNLF
                     VFFMLTLVLANNYVMLFVGWEGVGLCSYLLIGFYTLRKTASDAANKAFIVNRIGDAAF
                     VIGMLLMFATLGTVQFTQVTDILRHATYHPGSERYFNLIALCLFIGATGKSAQIPLYV
                     WLPDAMEGPTPVSALIHAATMVTAGVYMVARSNALYVLAPDTMHLVAIIGAATAIFAA
                     SIALVQTDIKKVLAYSTVSQLGYMFLACGVGAFWVGVFHLYTHAFFKALLFLGSGSVI
                     HALSGEQDMRRMGDLRDKLPVTFRTMFIAALAISGIPGFAGFFSKDEILWQTWTSENG
                     AHRFLYIMGLITAGFTAFYMWRLIHMTFYGRSHVAPEAAHHVHESPRTMTLPLAVLAA
                     GSIVAGWVGIPNAWHFPESWPKLEHWLAPVFADRPEAIAAAAESHSTADEWYLMTISV
                     VFALGGIFLARRMYHHKPELADGMERRLKPIHVLLYNKYYVDEIYNALFVDGLAKGGG
                     ALLSRFDAAVVDGGVNGAGWLTRFTSRVSMWWDTWVIDGSVRLGSFLVKLASYPVRIL
                     QTGLVQSYALFIVIGMALFLGYYLWR"
     gene            complement(17089..17397)
                     /gene="nuoK"
                     /locus_tag="VFA54_09475"
     CDS             complement(17089..17397)
                     /gene="nuoK"
                     /locus_tag="VFA54_09475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HZS51078.1"
                     /translation="MHPITVVHYIFLSAALLAVGAIGVLTRRSIVVILMSIELILNAV
                     NVNLVAFSKYLGNINGQVFSIFVITDAAAEAAVGLGILIALFRNKETVNADEVDILKW
                     "
     gene            complement(17405..17917)
                     /locus_tag="VFA54_09480"
     CDS             complement(17405..17917)
                     /locus_tag="VFA54_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HZS51079.1"
                     /translation="MIDTFLFYVFALLALGGGILTITRRNTVHSAIWLITSLLGVAGL
                     YLLQGAEFLAGVQVILYVGGIMVLFLFVIMLVNLGQESRQKPFTGQRWIALACIAALL
                     VQTLLFVKAGSGQFRLGEPGGGFTVQGLGNSEQISRTLFSQYLLPFELASILLLVAII
                     GAVVMAKKRI"
     gene            complement(17957..18412)
                     /locus_tag="VFA54_09485"
     CDS             complement(17957..18412)
                     /locus_tag="VFA54_09485"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HZS51080.1"
                     /translation="MKKFLRTIFLVDLLEGLWVTFRNQKPEYIYTEQYPAERPKVAER
                     YRGAPRLNINPDTGETLCIACNLCALACPEHLIVVTSERNEETRRKELTTFTYDTSRC
                     MFCGLCEDACPVDALELTQDFELATYTREGQIWDRQMLEEGPRPKQYTH"
     gene            complement(18441..19628)
                     /gene="nuoH"
                     /locus_tag="VFA54_09490"
     CDS             complement(18441..19628)
                     /gene="nuoH"
                     /locus_tag="VFA54_09490"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZS51081.1"
                     /translation="MPETAALLQIYLKPLILTAVIILVFPLVAGYIVLAERKVLADFQ
                     VRLGPMRVGPYGFLQPLADAVKLLLKEDIIPSAADRWIFWLAPVLSTVTALAAFSVLP
                     LGPGLHVADVNIGLLVILAFSSVGILGIILGGWASNSHYSLLGSLRSAAQMVSYEVAM
                     GFAIVSGLMVAGTLSMEKIIQAQQERHIWFAFANYGLMLVPFVTYSIAAVAETNRAPF
                     DLPEAESELVAGFHTEYSGFRWSLYFLAEYANMFVVSSIAVTLFLGGWLRPFPSIAAL
                     HYPLDFGFPALLFGLIGFGCAIGVMRRSLAVDKAIMAALAGAMFVLAAAFLAPAVNQA
                     IIGVFWFLFKVSVILYCFIWYRGTFPRYRYDQLMRVGWRYMIPIGLGSLIVNGVVLLL
                     AQR"
     gene            complement(19642..20754)
                     /locus_tag="VFA54_09495"
     CDS             complement(19642..20754)
                     /locus_tag="VFA54_09495"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZS51082.1"
                     /translation="MPETTFLDANELVLNMGPQHPSTHGVLRVILKLDGEKVLGCDCV
                     IGYLHRGVEKIAENRTYVQFAPYVDRMDYVAAVSNGLGYCLAVEKLMKIEVPPRAQAV
                     RVILTELNRITSHLLWLGTHALDIGAITPVFYTFREREEALKIFENYCGARLTTHAFR
                     IGGLQYETYDTFEKDVLAFCDMFPAKIDEYEELLTGNRIWVGRTKGVGVLTAEQCKEY
                     GVTGPVLRAAGVPWDLRKAQPYSGYEKYDFEIPVGQNADTYDRYIVRMQEMRQSVRII
                     RQAVEGLPTGPIMGKVGKVLKPPTGECYVSIEAPKGELGYFIVSDNSTQPYRVRVRPP
                     SFVNLQALDRMCRGSLIADVVAIIGTIDIVLGEVDR"
     gene            complement(20860..21369)
                     /locus_tag="VFA54_09500"
     CDS             complement(20860..21369)
                     /locus_tag="VFA54_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZS51083.1"
                     /translation="MAASPWQSPLTEKLKSRFGESIREFSTYLDQNFLIADLPAVVPI
                     LEFLKVEEEFEYLVDITAVDYPKREKRFDLVYILYRFPPHNERIRVKTFVADGEKVPT
                     ATTVYLGADWLEREVYDMFGIVFEGHPNLKRILLPEEWQGHPLRKEYGIIQQDVRWVQ
                     ENLQIESGQ"
BASE COUNT         4809 a         6712 c         6358 g         3974 t
ORIGIN      
        1 cagaatcatc ggcagaacgt agaggatccc gagagacaca tttacgccga cataccagtc
       61 ggcgaaagcg ctcaaagcaa ttaagatcgc tgacacgata agaaccttag ccctgccggc
      121 tttcaaggcg atggcgatca ctctctgcgc gaccggactt tgaaagctca gggccatggc
      181 tgccgtcaaa cttctatata actcttagca ggccaatttt gcagaggcgt atgtggtccg
      241 aaagaaaccc gactttgacc gaaagatagg ctcccgaagg gcccgttcct gtctagtatg
      301 gctgcggggg ttttcggggt gagcgcccta aaattggcgg cgttctttca gacaggcatt
      361 ccctgagata tcgattgggc gcaacctggg cccgaggtac ggtcttctgg gttgtaatcg
      421 ggaacaagag tggcccgtcc ggcttttttt caggagagca ggggaaacaa gtccctggga
      481 aaacgcagcg cgctccggct aattcactcg agccaaaacc actactgacc ttagcgtaga
      541 tggaaggcca gcatcagagc gagactgccg gcgccgctga gaaactccag atacgcgcgc
      601 ctggctttgc ggtatttctc aaatgcggat cgctccacca agcgctgtct ttcgattgcc
      661 gcccattcct cggccgtggg gcgggccccg gagaaacgca acaattcgag actcttctcc
      721 acctggcggg agtattcggc aagagcttga gcccaggctt cttgtaagtc ggacctgtcg
      781 cccgtcattg ggatcagaat agcagcctca ttcgcctttg tcgatgctca cacctgcgtc
      841 gtgtcggcca tgaacgcttt cctcgtgttc ttgaatttcc tgccaggttc tctcgcggcg
      901 cgccgccgca agctcgatcc gcccttccaa ttcttggatt gcgtcgaagt cggtgttgcg
      961 tgaaggcatg gagtgcatga gttccaattg gctctgcgtg gcctccaggt atgcgcgcca
     1021 aaggcgagaa cattcagaac aggttggcgt tttcttgttg ttatttcgat ccaccattca
     1081 cctcgcctaa gcttcgccgc cgccggctag tttacgctcc aggcgcgccc agcggatcgc
     1141 cgccacgcgg tactcgaatt gccgggtgca ggccttattc caaggtacga tgagatacat
     1201 ttgggtcata acccattgaa agcatacgtc tcttcgtttg taattctacg ctcatcggta
     1261 aagtttacgc gactgtggta ttggaaacca aacggttgaa aattctgcgg gcatcctgga
     1321 agaaaatgct ggaatcttgc gccgcagtgg cgggcagcct aggcgaaagc agatggttgc
     1381 ttaaggggta aagagcggaa ggaacgaggc cggggccggc gttgtctcga acggccggta
     1441 gtgtggcttc ttttagcgga aaacgcctgt atggccgagc gagtagcgcc caggctgggg
     1501 atagacgcac aggccgtggg gacccttgcc gacccgaatg cgggccagca aatggccgtc
     1561 gctggtatcg atggcgtaga cttcgcggct ataccgcccc gaaagccaca ggactttccc
     1621 ttcggccgag actcctccca tgtctggggt gccgccgccg ggcaaacgcc acttttccac
     1681 gattttccga gatgccagat cgatcaccga aatactgccc tcctcgcggt tggaagcgta
     1741 gagcagccgg ccatcccggc tgacgtaaag gccatgcacg cctttgccgg agggaagcga
     1801 gcctgcgatc gtaaagttgc tgctgtcgat ctcgtaaacc acattggctg ccatatcggc
     1861 cacatagaaa atcttgccgt cgggtgagag cttcacgtcc tgcggcatgc cgcccggcgc
     1921 gaggggcagc tcggcgacga ccttctgttc agccacatcg acggcaatca cttcggcgga
     1981 aaactcgcag ctcgcgacca gatagcgccc attggccgag aaatccatat ggtctacgcc
     2041 ccggcaaggc acaggcgtcg aatgcacgag tttcatggtc tgcgggtccc gaaagtcgag
     2101 acggtggagg cgctcggcga ctatgatcgc gtatttgccg tccggggtat agtacatgtt
     2161 gtacggatct tcaacccgga tggtttttcc cttctttcct gtggccgggt cgatctcggt
     2221 gacgctgtct ccaaggtcgt tcagcaccca cagctttttc atatcgtaag agggcgtgac
     2281 gtgctgcggc tgccggccca ctcgaaaatg atccacaatc ttgtaagtct tgggatcgat
     2341 cacatcgact gtattactgc cgctgttggg cacataaacg agcggccgga agttgcgaac
     2401 ctcgggactg agattgcccg ccgagtcggc ggcgtaaatg ttctccggat tgagcaccgg
     2461 aggcatacca ggcaacggcg gcgcctgggc cagcgcggcc accgcggcag agggacgcct
     2521 ggcgcctgcc accggagcgg cgtgcgccga gtggtcggtg ttttccgtct ttccattgcc
     2581 gcaggcgggc tgcatcagca gcacggcaat gagaaataaa ctgacagagg aaaaaggaaa
     2641 ttcagaccgc atgaggttgc gcgcaaatca gcattacatt ctaaactgga cctcgtacgc
     2701 cgccgcaaaa aattcgtttg atgcgaatca tcaccagcgc cgggcgggcg gttgtggccg
     2761 tccatgcgca tttgttgtac gctgagatca agggacgaag gctatgaact actatgccgt
     2821 gcttggcgtg gatgccgacg ccgacgagaa gacgattcgc agcgcctttc gggcgcttgc
     2881 ccgacggtat catcccgatg ccggagaagg gtcctcggag gccaagtttc gacaggtcct
     2941 ccaggcttat gaggtgctca gcaaccctgt ccgccggagg gaatacgacc agtcgcaggc
     3001 tgctctgaag acgcgtccgc cgttctcctt tggagaagta cggcgcccgg ctggcgtatc
     3061 gccggcggcc cccctcaggc aacgtgcctg ggatccgttc ctggaggccc gcacctcgat
     3121 cttcgagatg atgcaggtgt ttgagcgcct gtggcgatgg gacgaagatt ttttcggcga
     3181 accttttttc taagtttgta atttgagcgc ctgccaaggc gtttatcagc gctcgcggcg
     3241 ggcagggtag agctgcaaag ccagccggcc cgcttctctc atcgaaaatt ctgagacttt
     3301 cttcacacga gcgaggggaa tgcttacggc ctgactcgct tcggaatccg gttcgggaaa
     3361 tagacggata tcgagagcct cctctcccgc ctccacttta gcggctgttt tctcgggaag
     3421 aaacccgatc agcgccactg aactgtcggg cagtttgtgg acctcgaggc tgtgatagat
     3481 gcgctttctg aacaggggcg aattcgcgat gccgggcgag tacgtgaagc catacacgcc
     3541 cggcgggagc cgctccagac cgaagccctc ttcctcctcc gttaccaatc tcacgccgtt
     3601 ttgggctcgg agcgcttccc gctccgcctc cccggcggcc tccggcttcg ttagttccgg
     3661 tgcagccgca actccgcctg ccttggactc tcttttcatt tgtgctattg cgctgccggt
     3721 gttcttacaa tctcaacaac ccagcgggat aattcattat aaataatttg cgaaaacgct
     3781 aatgctcaat cccccaggga cggggagctt ccattctcca gttccgttct gggatgaggc
     3841 ctgccggttg acttgcacct ggccgggagg catcatgttt tgtgaatcga ggaggaaaga
     3901 tatgacttcg aagcgattgg caatgtgcgc agccctctgc gccgctgtgt ggtcgatgcc
     3961 gagaatggcc tgggcgcagc agccggccgg gtcggttcgg atgtataaca cggcgaagca
     4021 gaaactgctc tctggcaagc agattttcgg cggaaccgcc acttcaccgg atcccaatat
     4081 ctattgcgcg ctggcgaacg ccggctttga tttcacctgg atcgagatgc agcacagccc
     4141 gatgacctac gaggacgtcg cccgaatggt ttgggcctgc cggggcagtt ccgcgatacc
     4201 gttcattcgc gttccggatg cgacggaaag cgatattcag aaagctacgg atctgggagc
     4261 cctgggcatc attgtcccca cagtggatac cgttgagaaa gcccaggctg ccgtcaaatg
     4321 ggcaaagtac ccgccgcagg ggcgccgcag ccagggggcc ggccatgcca ccgctatctg
     4381 gggcagcgat taccgccaga ccgtcaacga caatatgatg gtcgtgatca tgatcgagac
     4441 gcccaccggc gtggaaaacg cggaaaagat cgcatccgtg cccggcatcg atgtgatttt
     4501 cgcggccagc ggcgatctgg gaaacttctc cggccacttc atggacccga agacgcacaa
     4561 aggcgattcc tactatgaat cactagtcac cagaatccac gacgtgacct tggcgcacaa
     4621 catcaagctt ggcggcccgg acgtatggaa ggaccgtccc ggctacagct tcttccaaag
     4681 cagcagcgag ccgggcctga tacgagcggg cgcacaagcg cttggcttca agcccgttcc
     4741 ctaccccgcc cagcgccagt aggttccatc attggccgct gcggtgccgg gtccacagca
     4801 gcggccttat ttgcgatcaa aacgccagga ccagcgccca tctcctttga ccatcgcgca
     4861 aagccgtcat tccgagggaa cgggggaacc ggctcttacg cgaggcggcg aaacgccgga
     4921 tcgaagtctt ggcagagaca agtttggcgc ccgcaggaat gatcggggct aacccccggc
     4981 ggctatctgg ggagggcgga tatgctttac ttggccggcg tgttcattcc tccgagccgc
     5041 tgcacgcccg ttccagcttt aggcacaggc ggcgcgcggc tgcgtcgtcc agtttccggc
     5101 ctttagaccg cacgtagaac acgtcgatcg ccttgcgccc ttccgtatcg atcagcacca
     5161 catcgatatc gcagcgatta aacgaaaacg tgctggcgag gtcataaagc aggccgggcc
     5221 ggtcctccgt gatgacatgg aagagcgtct cgttcggaga cgtttggtta tcaaaggcga
     5281 tgtggcgggc gatgcgcggc cggtgagtag cggccggcgt ccgccgtttg tggagcagct
     5341 cgcgaacgtc aatcttgccg agcacgacct gctcgagagt ggaagtgagc cgatccgctt
     5401 cgctggggtt cagctccaga gtgcggttgg gatcggcgaa gatgaaggta tcgagaatca
     5461 tcccatcccg gttggagaac gcctccgctt tcacgatgtt catggcgaac gaggaaatcg
     5521 ttcctgcaat ggacgcgagc aggaaagcgc ggtcgcgggc gagcaccacg agttcataca
     5581 gagagtgccg ccggccaagc tgcacggcca cggaccggcg ctccaacacg ggcttcaact
     5641 ggaaatggcg gcggatctgg tcggggccgt gaatgcgcag ataccgcgtg gggagtccct
     5701 cgagaaagcg ggcgagagcc tggcgctcgg agtagttgga tgccagttgc gcggcgaccg
     5761 cggcgccggt gccgatccgc tcggcttcca tttcgcgggt caactggtta tgtgtggcga
     5821 cgtagagctg ccataacaga tccttgcgcc acggcgtgag ggcttcgggg ttcaccgcgg
     5881 aaatatccgc gtatgtcatc agcgtgaggc tttggagccg ttcgagcgtg ccgacctgcg
     5941 aggcgaaggc gcgcgccgtt tctgggtccg ccaggtcgcg gccggtcatg atggccggca
     6001 tgtcgaggtg atgcgcgatc agaaaccgca ctgtttcgcg ctccggctcc ggcatgccga
     6061 tacgctccat cgcctgttcg gcgagggcta atcccgcagt cgtatggttt ccttcccagc
     6121 cgcctttgcc cacatcgtga aacagcagag cgaacaaaag gacctccggc cgttcagttt
     6181 cctgcatcag ctcggaaaaa tgctgcgtca acgcgtcgtc gctgccggcc cgcagctcgt
     6241 gcagcgtttt gatcgtccgc aacgaatgct catcgacggt gtaccggtgg tagaaatcgc
     6301 gcaccaccag gcagtcgatc cgctcatatt cgggaaaaag cgccgctagg aggcccgttt
     6361 cccgcatcac caccaaagcc tggtaagcgt gcggcagccg caggatcgac gcgatagaga
     6421 accacagccc cgaagaggcg ccgcactgtt tgcggaggta ttcacggaag tttcccgccg
     6481 ctccgccgat gcgccgctcg gtctcgggcg ccggcggaac cccgtgccgg gcgataaact
     6541 caaacagccg catcgccaga ccgggattct gcaccagcgc ttccggatgg cgcatgtaca
     6601 acatgccgcg cgcaaccgcg aaatcaggat tcgacagccg cgatttgcgg tcgcggaaca
     6661 tcgtgaacag cgaatgttcc ggcgtggcga cttcgtccag cgtccgccgc gcgagccggt
     6721 gaatgacgcg cacattgcgg aaataatcgc gcatccagac cgcggcgtcc tgcggggatg
     6781 cgccggcgcg ctccgccagc cgctcctggt tctcaaaggt cagaatgtta ttatcgcgcc
     6841 cattgaagca atgcaggtag caacgcagcg cgaaaagaaa ccgtttcgct tccatccacg
     6901 catcgcgcgt ctcggcggga aggtgctgct cgggcgggct cagggcgaag ccgcgggcct
     6961 ccggcggaac agcgtttgcg gccgcttcct cggcatgcgc cagttgggaa agccagcagg
     7021 cgacttgaaa atcccgcagg ccccccggcg cttccttgac attcggctca agatggtaga
     7081 tcgtgtcgcc gtgcttagcg tgccgcgccc gcgtcagccg gtccagcccg cgcgccaagg
     7141 tctgtttctc gcgcgcgtaa aaacctggca ggcgctcgaa cagccgggcg agggccgcca
     7201 tgtccccggc gagataccga gcgtcgagca agctgatatt cagctcgacg ttatccggag
     7261 cgagcgaggc cgcttctgcc ggcgtgcgca ccgagtgaga cgcccgtaca cccccgtccc
     7321 agagccctcg caataacttc gcaatgcggt cgcgggcggc ctcggcggca gaatctcttt
     7381 cgaagagaaa gagcagatcc acatcggaac agggaaatag ctggcgccgc ccgtagccgc
     7441 ctaccgctac cagagcgacg ccggaagcgg cctgctcgcc gaaagcttcg tggaacagag
     7501 cggcgactgc acggtccacg gcatcggagc gctgccgcac catctccaac ccgtcgccgg
     7561 tttcaagaaa cttctgcttg atcggctcca ggccggcgag gagtccgggc gcacccgccg
     7621 tcatagcgct ccttcgccgc gatcgttgtt gcgaatccgg atagcgtcgt caatggagga
     7681 aatgaagatc ttgccgtcgc cgagcttgcc agttcgggcc gcgtcgccga gcgccttcac
     7741 cgtctcttcc aaacggctgt cgggaaccac gatctcgatc ttcactttag gcagaagatc
     7801 gaccgtgtac tcctggcccc gatacacctc gcggtggccc ttttgccggc cgtggccgcg
     7861 cacgtccgaa atggtcatcc cgtcgatgcc cacttccttc agcgcttctc tcacgtcttc
     7921 cagtttgtgc ggctgaatca ccgcctcgat ctttttcata acggagccgt cctgtcatgt
     7981 gtcctgccgc ttcgcggctg tgccttgaag acacggctgg gggcgcctgg tcccggcccc
     8041 ttgcccgcag ggtgcggccc ggctcgcaag ccggaccgga caggcgcgaa gaggcgctaa
     8101 ccttcaaaat tgtagccttc ttctccgtgc tgggagaggt ccagaccctc gatctcgtct
     8161 tccttgctca cacgcacgcc gagcagggca tccacgattt tgagaatgat aaacgtcgcc
     8221 agagccgcca ggagccaaca gatgagcacg ccccacaact ggttgaggat ctggccggcg
     8281 ttgccgtcgg caagccccag cgcgcggctg tggccgctgg ggtcctgcat tgtattgttg
     8341 atagcgttgg tggcgaagac cccggtcagc agagcgccga gcgtgccgcc ggctccatga
     8401 acgccgaaag cgtccaggga atcgtcatac cccattttac ccttcaccac catcaccatc
     8461 aggaaacaca ccagtccggc gatcagcccg atgaagagcg ccgacatcgg ggcgacaaag
     8521 ccggagccgg gcgtgatggc caccagaccc gccacggctc cggagattgc gccgagcacg
     8581 ctgggtttgc cgttcttgag ccattctcca atgctccagc cgagcgccgc cgcagccgcc
     8641 gcgaaatgcg tgttgacgaa agcgctggtg gcaaggcccg aagccgcgac ggcgcttccg
     8701 gcattgaacc cgaaccaccc tacccaaagc aacgatgcgc cgatcacgct cagcacgacg
     8761 ctatgcggct tcatcggttc gtgcggatat ccgtagcgct tgcccagata gagggcgcaa
     8821 accagcgccg acacgccgga agaaatgtgc accacggtgc cgcccgcgaa atcaaagcag
     8881 ggaattttgc cgccgagcga ggcgttcagc aatccgcctt tgccccacac catgtgcgcc
     8941 atcgggaagt acacggccag cgaccacaga atcatgaata acaccatggc gctgaatttt
     9001 atccgctcgg cgaaagctcc ggtaatgaga gccggggtga tgatggcgaa catcaactga
     9061 aacatcatgt aggtctgatg aggaacggtc gccgcgtaat cggcgttcgg cgccagtcct
     9121 acgccgtgca agaaagcgta gtggaggccc ccgataaacg cgttgccttc gctgaaggcc
     9181 aggctgtagc ccaccagcgc ccacaggacc gtcaccagtc ccatgagaaa aaagctttgc
     9241 atcatcgtgc tcagcacgtt cttggtcctc accagccctc catagaagag cgccagcccc
     9301 gggccggtca tcatcaatac cagtgcacag ctcaccagca tccacgcgtt gtctccggcg
     9361 ctctgggcgg tcttcagcgc cgtttccatt ttggccagcc ggtccgacgc gggatcgggg
     9421 ccggctgccg ccgtctgcgc cgcggccggc cccagcgaac aggctagggc gagcaataac
     9481 gcaagggcac acactacccg catgggtcgc ttcatgccta actcccatcc atcatcttgc
     9541 aacagggagc ggcaaagaca aaacgaaagt ttctatcgag gtgattgatt atttctatga
     9601 aagggggccg ggccgccgaa tatcgggcgc gatcccggcc catgatgcga tcacgacact
     9661 ttgacgaaaa taatcaccag cgtatagagc gccagcgact cgatcaacgc cagtccgaga
     9721 atcagggcga aacggatggc tgcggcggcc gccggattcc gcgccatgcc ttccgccgct
     9781 gcggccgtgg ccctggcctg ccccagcccg cagaggccgg aggcgatcgc catggcaaaa
     9841 cccgccgtaa tcacgaccca ctggtgcgaa gcgtcggccg gcgggccctg ggcgaacacc
     9901 ggagcggacc agcaaagcag cgccgcaaac aacagcaaaa tggaaaggac tctcattttc
     9961 ttctcctgtc tataaagtgt cttccggagg tggttcctgg acacccctct tgcagccagg
    10021 ctcacgcgga agagaatcat gccggaaaaa accgcgctcg agcgccgctc gtggcgagcc
    10081 ccaatgcttc cctgcacggc agcgcaagcg cccgttcagt gttcgtgcgc cacggctccc
    10141 tgcacgtaga ccatcaccag caacatgaaa atgtaagcct gtaagagaga aacgaagacg
    10201 tgaaggccca tgaaaatcgc tggaatggcg atggggacga ggccgatgaa aatcatggtg
    10261 accatttcgc cggcgaacat gttggcgaat agacgaatcg tcagcgacat cacccgagcc
    10321 gaatggctga caatctcgat ggggaccatg agccaggcca gccaccagac cggcccggca
    10381 aaatgcgcga gatatttccc cacgccctgc gcccgaacgc cctcaatgtt gtaataaaga
    10441 aacgatgcca gcgcgcagcc gaagggcacg gggccgggaa tgtattgggt aggcgagccg
    10501 aatccaggaa tgattccgag cagattggca aacaggataa agataaacag cgtgccaaag
    10561 aacgccagat accggtgcga gccgtggccg acaatctctt caccctggcc tgctacgaac
    10621 tcgtaaagca cctcgaacga ctgctggaaa ttgccgggcc gctccacggt gaggcgaccg
    10681 cgaagcagcg cgaacaggac aataatgatc agcgctacca ggatttgcat ggaaatgaaa
    10741 tcccgccacg ggcgttccgg attggcggcg tgcagatgaa acagcgccag aatggcgttg
    10801 gccaagcccg ccaaataccc gttcagcagc ttcgtaaacc acagttcatg ttccatgccg
    10861 gtcttgcttt agccctatcg cgatttccac gcagatttcc gccagaatgg cggccacgaa
    10921 cagaaacagc cccgccagca gcgccttgac gctccaggca gtatatctga atgtagcata
    10981 ggccgccgcg gcaatcaaag cgtaccgccc caaaaacctc cggatcacac gccgtacggt
    11041 ccggcgggca agtcccgcgc cggcaatagg cggcaagccc tgcacatcgg ccacccccgg
    11101 ccgccctcga gtctgctccg ttccggcctg ccgcgttccc agatccgcgg cggcggctct
    11161 tgcggccagg gtgtccaccg cctgcttcag ccagaaaaaa ttaaccgcgg aaatcacggc
    11221 ccccatcgtg aagccaaggg cgccgcgggc gccggccaat cgccaggctc cgccgacgcc
    11281 gagaactccg aaggcaagct gaaagcgcca gatgcgccgc tcggcggcgg cgtagaaatc
    11341 cgaggctgct tggtccgcgg cagggtcagc gtccattgcc ggctctcgct ttatcgtacc
    11401 cgcaacgtga aatgccggtg acgcttcccg gcttcgaaaa tgggcgcgcc atcttcgatt
    11461 acgctttgcg aaccggcctt ccccgcggaa cggccggcct ggaaagctat tgaaaattgc
    11521 atccggaatg cgcctttccg gccgcgccgc gcggcgtcca tcttcgaatc agtgatctcc
    11581 cgcatccttc tgcacctgcc ggatcagctc gatgaagccg cccgcgatcc ccagtatcag
    11641 gaagacgata gacaggaagc ttgtgtgaaa caggcggtcc agaccgtagc cgatcagata
    11701 gccggcggcg gtgcaagcgg gcaaggtgaa agcaagcgaa gtgtattccg cgacctgcac
    11761 ccacgggttt ttcttctgct gtggcatggg caagcaggat tgggtggcct ggcgggctcc
    11821 gcggccgccg aagccggtca ccgcagtgcg gcgaacagcg agcgctgcgc catctgaaga
    11881 aacggctcgg gataaacgcc gatcagcact gtaaacgccg cggtgacgcc caaggcgagg
    11941 cgcacgccca ggctgtcaga aagttccacc ggggccgccg cttcttcgct cacgaacatg
    12001 acttttacga tgcggaagta atagtacaga gctaccgcca catagagggc cgcaaacacc
    12061 gccagatagt aatgctgcgt ctgaatgagg gaaaggaaaa tgtagtattt tccgaggaat
    12121 cccgcagtgg gcggaatgcc tgccagagag agcaaaaaca ccagcatcag cacggcataa
    12181 gtcggatgct tgaacatcaa gcccgacata tcatcgatct cgtcgccgat gatattcttg
    12241 ttgcgcattg ccactacgat cgtgaaggcg ccgaaattca tgaaagtgta aaccaggata
    12301 taaacggcga tgcccttcag cccggtctga ttgccgctca ccaggcccag cagaatgtac
    12361 ccggcgtgcg aaatggaact gtaggccagc aggcgcttga tgttggtctg gctgatcgcg
    12421 gctaggttgc cgagggtcat cgtaagcagg gccactgccg ccaccagcgg ctcccaagtc
    12481 gcccgcatcg attccagcgg cacttgaaag attctcagca aaaatgcgaa cgacgcggcc
    12541 ttcgatgcga cggaaaggta cgccgtcacg gttgtcggcg ctccttcata agcatccggg
    12601 gcccacatat ggaacggcgc agcggctatt ttgaaaaaca gccccacccc ggtggtcgcc
    12661 atcgccagaa ataccagcgg gtcgcggggg tcccggccgg cgatcgcgcg cgcgatgtcg
    12721 ccgaggcggg tcgagcccga aatgccaaaa aaaatggaga agccgtaagc caataatccg
    12781 ctcgaaaaag cgcctaaaag aagatatttc atggcggcct cgttcgaacg cttgtcgccg
    12841 cgcagaaatc cgaccattac atagaaactc aacgccatca gttccaggcc gatgaacaga
    12901 gtgaccaatt cggcgcccgt ggcgaggaag accatgcccg tctgggcaaa cagcagcaaa
    12961 gcataatatt cgccgtgatg ctcttgctca atctcgaggt accgcgtgga gatgatgacg
    13021 gccagggcgg tggcgatcaa gaagatccaa tcgaagaaaa tcccgaaccc atcgacggtg
    13081 accgctccgg aaaggcccgt caattcagcc aggccattcg cccgcaaaaa actctgctgc
    13141 tgccagaccg cataggccgt gaagcccagg ccgatcaggg ccgtgatcgc attgaagaat
    13201 ttttgccgga cgtcgataaa gaagtccgtg atcagaatgc cgcagccgaa aaggcacagc
    13261 aagatagcgg gcttcaggac aaaaaaatca gtttgtgtga aggcgaaatt catggagttg
    13321 cgatctgcgc cacctgcccg ggctgttcaa gcggatcttt caagttacgc acgcgatagt
    13381 aatcgggctg gacgcgcttc acgattttgg cgaccggctt ttcgagaatc tcaaaatacg
    13441 gcttgggata aacgccgatc cagaacgccc aggctatcag cggcagcatg taggcgatct
    13501 cgcgcgcgtt gagatccttg aggtgctcgt tcttagggtt cgtaatgggg ccgaacatgg
    13561 tgcgctggta gagccagagc agataagccg ccccaagtac gatgccggaa acggcaaagg
    13621 cggcccacag cttggatacc tcaaacgccc cttgcaaaat cgtgaactcc ccgacaaagc
    13681 cgttcagcag cggcataccc gccgagctca gcatcgcgat cacgaaaatg acggcgtaga
    13741 ccggcatcac gtttgacagc ccgccgtatt ccgatatttc gcgcgtgtgc cggcgctcgt
    13801 aaataatgcc gatgatcaag aagagcatgc cggtcgatat gccgtgattg atctgctgga
    13861 tcaccgagcc cgtgaggcca ttcgggttca gagcgatgat ccccagcgta cagaagccca
    13921 agtggctgac ggaggaatac gccaccagct tcttccagtc cttctgcatc agggacacca
    13981 aggccccata gatgatgccg ataatcgaca gcgccgcgat catcttgacg gtgaaaccgt
    14041 ctttagcggc atccggcagc agaggcagcg agaagcggat aaaaccgtag gttcccatct
    14101 tcagcaaaac gccggccaga atcacagagc cggccgtggg cgcctcgacg tgcgcatcag
    14161 gcagccacgt gtggaaggga aacatcggca ccttgatcgc aaagcccaga aagaacgccc
    14221 agaacaccca tttctgcatc gtgagcggga tgctcatgcg ggtgagttcc tggatgtcga
    14281 aggtatacgt tccgaactgg ctatagtagt gatagtacag ggtcaggatg ccgagcagca
    14341 tcagcaccga cccgaacagg gtgtacagaa aaaacttgat agcggcgtag agtttccgcg
    14401 gaccgcccca cacgccgatg atgaagtaca tcggcaccag catcacttcc cagaagacgt
    14461 aaaacaggaa gaaatcaagc gccataaaga cgcccagcat gccggtttgc agcagcagga
    14521 aaaaggcgta atactctttc acgcgatcct ggatcgccga ccacgaagag agcaccgaga
    14581 taaagcccac cagggtcgtc agcaaaatca gcagcaggct gatgccgtca atgcccagag
    14641 cataatgcgc gccgatggac ggaatccagt tggcccgctc gacaaactgg tagtcggcgt
    14701 tcgaccggtc atacatccac cagagcggga cagacaggat aaatccgaga aacagcacca
    14761 ggttcgccca gacgcggatg gccgtcttgt tctccttcgg aatcagcaga agaatgaaca
    14821 tgcccgccag tggcgtaaac aacagaatgc tcaagtaatg ttgttgcata tcaaatcgcg
    14881 gtcatggccg ccggcattgc gccgcttccg agccgcctcg cacgagaggt ctacgtttta
    14941 atgcagagcc ttcctcccga gagaactcct atctccacag gtaatacccc aggaacagcg
    15001 ccatgccgat cacaataaac agcgcgtagc tctgcaccag gccggtttgc agaatgcgca
    15061 cggggtatga cgccagcttc accaggaacg agcccagccg caccgagccg tcgataaccc
    15121 aggtgtccca ccacatggac acgcgcgagg taaaccgggt cagccaaccc gctccattca
    15181 ctccgccgtc cacgaccgcc gcgtcaaagc ggctcagcag cgctccgccg cccttcgcca
    15241 gcccatctac aaacaaggcg ttgtaaatct cgtccacgta atacttgttg taaagaagaa
    15301 cgtggatcgg cttgagccgg cgctccatcc catcggccag ctccggtttg tggtgataca
    15361 tgcgccgcgc caggaaaatg cccccgagcg cgaacaccac ggatatagtc atcaaatacc
    15421 attcatctgc ggtcgagtgg ctctcggcag cagccgcaat ggcttccggc cggtctgcga
    15481 acacgggagc cagccagtgc tccagcttcg gccaggactc cggaaaatgc caagcattgg
    15541 gaatgccgac ccaaccggcc acgatgctgc cggctgccag cacggccaaa ggcagcgtca
    15601 ttgtcctcgg cgactcgtgc acatgatgcg cggcctcggg cgcgacgtgg gaccgcccgt
    15661 aaaaagtcat gtggatcagc cgccacatgt aaaacgccgt gaagccggcg gtgattaatc
    15721 ccatgatgta taggaaacga tgggcgccgt tttccgacgt ccaggtctgc cataagatct
    15781 cgtctttgga gaagaaacca gcaaagccgg ggatgcccga aatcgccagc gccgcgatga
    15841 acatggtccg gaaagtcacc ggcagcttgt ctctcagatc gcccatgcgc cgcatatcct
    15901 gctcgccgga gagggcgtga atcaccgacc cggatccgag gaacagcagc gctttgaaga
    15961 aagcgtgcgt atacaggtga aaaacgccga cccagaaagc gcccacgccg caggccagaa
    16021 acatgtaccc gagctggctc acggtcgagt aggccagcac cttcttgata tcggtctgga
    16081 ccagggcgat ggaggcggcg aaaatcgcgg tggctgcgcc gataatcgcc accaggtgca
    16141 tcgtgtcagg cgcgagcacg taaagcgcgt tcgagcgcgc caccatataa accccggctg
    16201 tgaccatcgt ggcggcgtgg atcagcgccg agacgggcgt cgggccttcc atcgcatccg
    16261 ggagccatac atacaaaggg atctgagccg atttcccggt cgctccgata aacaggcaca
    16321 gggcaatcag gttgaagtag cgctccgagc cggggtgata cgtggcgtgc cgcagaatat
    16381 cagtgacttg ggtaaactgc acggtgccta gcgtggcgaa catgagcagc atgccgatga
    16441 cgaacgccgc atcgccaatc cggttcacga taaacgcctt gttggcggcg tcgctggccg
    16501 ttttgcgcag cgtgtagaag ccgatgagca agtagctgca tagacccact ccctcccagc
    16561 ccacgaacag catcacgtag ttgttcgcca gaaccagcgt gagcatgaag aagacgaaca
    16621 ggttcaggta gccgaagaaa cggtaaaagc ccccgtcatg cgccatgtag ccgatggagt
    16681 agacgtggat cagaaatccg atgccggtga ccaccaggat catcaccgag ctcagcggat
    16741 ctagcagaaa gccccacgga gcctggaacg cgccgacgcc ggtgatccac ggggccagca
    16801 gcacctccgc gtgccgctct gcggccggca aggccgccag atcgagcacc gccttgaggg
    16861 agaacaaaaa cgcgatcagc accattccgg ggcagaggat ccggatcacg ctccggccgc
    16921 tgctctcgtg gtgtccatgc tcccggtgat caagagcgtc gtgttggcgg ccccactccg
    16981 gctgcgggtc gagcttgcgg ccgaacagca gcattgccgc cgcgccgaac agcggaaaca
    17041 gcggaatcaa ccagatgtgc tggaggaaaa agctggtttc ggccccgttc accatttcag
    17101 gatgtccacc tcgtcggcgt tgaccgtttc cttgttgcgg aacagggcga tcaggatgcc
    17161 cagcccgacc gcggcttccg ccgccgcgtc ggtgatgacg aaaattgaaa aaacctggcc
    17221 gttgatgttg ccaagatatt tcgagaaggc gaccaggttt acattcaccg cattcaggat
    17281 cagctcgatc gacatcagaa tcacaacaat gctgcgccgc gtgagcaccc caatggcgcc
    17341 cacggccagc agggccgcgc tcaaaaagat gtaatgaacc acagtgatcg ggtgcatgac
    17401 aatcctaaat gcgcttcttg gccatcacca ccgccccgat aatcgccacc agcagtagaa
    17461 tcgaggccag ctcgaacggc agcaagtact gggaaaagag cgtccggctg atctgctccg
    17521 agttgcccaa accttgcacc gtaaagccgc cgccgggttc tcccagccgg aactggccgg
    17581 agcccgcttt gacaaaaagg agagtctgta ctagcagcgc cgcgatgcag gccagagcga
    17641 tccatctctg cccggtgaac ggcttctgcc tggattcctg ccccaaattc accagcatga
    17701 tcacgaacag gaacaagacc ataattccgc ccacgtaaag aatcacctgc acgccggcca
    17761 gaaactccgc tccctgcaaa agatacaggc ccgccactcc cagaaggctg gtgatcagcc
    17821 agatggcgct gtgcaccgta ttgcggcggg tgatggtcag aatgccgccg cccagcgcca
    17881 gcagcgcgaa tacataaaac agaaaagtgt cgatcataaa aacaaccggc aaggggccag
    17941 gcgccggctc tcgccgttaa tgcgtatact gctttggccg cggcccctct tccagcattt
    18001 ggcgatccca gatttgcccc tcgcgggtgt aggtggcaag ctcgaaatcc tgcgtcaatt
    18061 ccagcgcgtc caccgggcag gcgtcctcac acagcccgca gaacatgcac cgcgacgtat
    18121 cgtaagtaaa cgtagtcagc tccttgcgcc gcgtctcctc attgcgctcg ctggtcacca
    18181 caatcaggtg ctcgggacag gccagggcgc acagattgca cgcgatgcaa agcgtctctc
    18241 cagtgtcggg attgatgttc agccgcggcg ccccccgata acgttcggcg accttggggc
    18301 gctcggccgg atactgctcg gtgtaaatgt attccggctt ctggttgcga aaagtcaccc
    18361 aaagcccttc gagcagatcg accagaaaaa tcgttcgcag gaatttcttc atacacttaa
    18421 ttctaccctg cgcatcgccc tcaccgctga gccagcagca gcaccacgcc gttgacaatc
    18481 agcgaaccga ggccgatcgg aatcatatac cgccagccta cccgcatgag ctgatcgtag
    18541 cggtatcttg gaaacgtgcc ccggtaccaa atgaagcagt acaggataac cgataccttg
    18601 aacaaaaacc agaacacgcc gataatcgcc tggttcaccg ccggcgcaag aaacgccgcc
    18661 gctaggacga acatggcgcc agccagggcc gccatgatgg ccttatctac agcaagagac
    18721 cgccgcatca cgccgatcgc gcagccgaag ccgatgagtc cgaatagaag agccggaaaa
    18781 ccgaaatcca gcggatagtg cagcgccgcg atgctcggaa aaggcctcag ccagccgccc
    18841 agaaacagtg tcacggcgat cgacgaaacg acgaacatgt tcgcgtattc cgccagaaaa
    18901 tacaacgacc agcggaatcc gctgtactcg gtgtgaaaac cggccaccag ctcggactcg
    18961 gcctcgggca aatcgaacgg cgcgcgattt gtttccgcca ccgccgcgat agaataggtg
    19021 acgaaaggaa ccagcatgag gccgtaattc gcaaaggcaa accagatgtg ccgctcctgc
    19081 tgcgcctgga taattttctc catgctcagc gtgcccgcca ccatcagccc cgaaacgatg
    19141 gcaaacccca tcgcaacctc gtagctgacc atttgggccg ccgaccgcag cgatcccagc
    19201 agcgaataat ggctgttaga ggcccagccg ccgagaataa ttcccaatat cccgaccgag
    19261 gaaaaagcca ggatcaccag caggccgatg ttgacgtcgg ccacatgcag ccccggcccg
    19321 agcggcagca cggaaaacgc cgccagcgcc gtcacggttg agagcaccgg cgccagccag
    19381 aaaatccagc ggtccgcggc cgaggggata atatcttctt tgaggagcag tttgaccgcg
    19441 tccgccagag gctgcaagaa gccgtacggg ccgacgcgca tcggccccag ccgcacctga
    19501 aaatcagcga gcactttgcg ctcggccagc acaatataac cggccaccag ggggaaaacc
    19561 agaatgatca ccgctgtaag gatgagcggc ttgagataaa tctgaagaag cgctgcggtt
    19621 tccggcatgg ttgctttccg cttagcggtc cacctcgccc aaaacgatat cgatcgtgcc
    19681 gatgatggcc accacgtccg cgatgagtga gccgcggcac atgcggtcca gcgcctgcaa
    19741 gttcacgaag gacggcggcc ggacgcgcac gcgatacggc tgcgtcgagt tgtcgctcac
    19801 gataaaatag ccgagctcgc ccttgggcgc ctcaatcgag acgtagcact ccccggtggg
    19861 cggcttcagc accttgccta ctttgcccat aatcggcccg gtcggcaggc cctcaacggc
    19921 ctggcggatg atgcgcaccg actgccgcat ctcttgcatg cgcacgatat accggtcgta
    19981 ggtgtccgca ttctgcccga ccggaatttc aaaatcgtat ttttcatagc cggagtaagg
    20041 ctgcgccttg cgcaaatccc acggcacgcc cgcggcccgc agaaccgggc ccgtcacgcc
    20101 atactccttg cactgttccg ccgtgagaac gccgacgcct ttcgtgcgcc cgacccagat
    20161 acggtttccg gtgagcagct cctcgtactc atcgatcttg gccgggaaca tgtcgcagaa
    20221 agccagcaca tccttctcaa aggtgtcgta ggtttcgtac tgcaagccgc cgatgcggaa
    20281 ggcgtgcgtg gtgagccggg cgccgcagta attctcgaaa atcttcagcg cttcttcccg
    20341 ctcgcggaag gtgtagaaga ccggcgtaat ggcgccgata tccagggcgt gcgtgccgag
    20401 ccagagcaaa tggctggtga tcctgttcaa ctcggtcaga atcacccgca ccgcctgcgc
    20461 ccgcggcgga acttcgatct tcatcagctt ctcgacggcc aggcaatagc cgagaccgtt
    20521 tgaaaccgcc gccacataat ccatgcggtc cacatagggc gcgaactgga cgtaggtgcg
    20581 gttttcggcg atcttttcca cgccgcgatg caggtagccg atgacacagt cgcagccgag
    20641 caccttttcg ccgtccagct tcaatatgac ccgcagcacc ccgtgcgtcg agggatgctg
    20701 cggccccatg ttcaaaacca gctcattcgc gtctaggaaa gtggtttccg gcataaacgg
    20761 ttgccagccg tcggctttca ggcgccagct ttcagcgcga ccttgcggcc gcctgtggcc
    20821 tttgctgagg gctgatagcc gaccgccggc ggcttctcct cactgcccgc tttcgatctg
    20881 caaattctcc tgtacccacc ggacgtcctg ctgaataatc ccgtactcct tgcgcaatgg
    20941 gtggccctgc cattcctcag gcagcagaat ccgtttcagg ttcggatggc cttcaaaaac
    21001 gattccgaac atatcgtaga cctcgcgctc cagccagtcg gcgcccaggt acactgtcgt
    21061 cgccgtgggg accttttctc catccgccac gaaagtcttg acccggatcc gctcattgtg
    21121 cggtggaaaa cgataaagaa tgtagaccag atcgaatcgc ttttcccgct tggggtaatc
    21181 gacagccgta atatctacca gatattcaaa ctcttcctcg actttcagaa attcgaggat
    21241 cggcaccaca gccggcaaat ccgcgatcag gaaattctgg tccagatagg tagagaactc
    21301 tcggatgctc tctccgaacc ggctcttcaa tttttcggtg agcgggcttt gccagggact
    21361 agccgccatt gcaggcgggg gtttgggcgg agcatgcccc gccggctttt gctcttctgc
    21421 catgtttcag tagggaccgg ccatccagtg agtgaactcc atagctgtaa cacagcagca
    21481 acgtgctgtc aaattccgcc ggactcgcgg cagcgggagc gctctctcct ttgagtgccc
    21541 tactgcctcc ttgaaactgg atgcgcgagc gccggaccaa gttcaatcgg catcaagcgg
    21601 ccggagcagc ggaccgggac cccggcactt tctacgcggc ggaggagaaa aagagtacat
    21661 gccggcaacc gtcagacaaa cgaacgcgct gctctggatt gattttgcga aagatggggc
    21721 gattcttttc ggcatcgtgt gttggcatac gatttgctcg acgatgctgc ccgcatcccc
    21781 agctcactcg cctccaatac aaactcgcgg ggcattttgc gatggtcatt gggcctcgat
    21841 gctatagcgc tcg
//
LOCUS       DASREK010000184         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1739515, whole genome shotgun sequence.
ACCESSION   DASREK010000184 DASREK010000000
VERSION     DASREK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739515"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFA54_09505"
     CDS             <1..475
                     /locus_tag="VFA54_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZS51084.1"
                     /translation="FDALAMRFRELKIRKNPDCPVCGEHRTIHHLIDYQEFCGIPHHE
                     PPPAPGLKEWEIDPVEVKAKMDRHEPFVLIDVREPHEYQICSIPGSRLIPLGELPKRV
                     HELNSADEIVAHCKSGMRSAKAVDFLRKAGFKKAKNMVGGILAWSDKVDPRVPKY"
     gene            631..1401
                     /locus_tag="VFA54_09510"
     CDS             631..1401
                     /locus_tag="VFA54_09510"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS51085.1"
                     /translation="MKNLRKRVLAGETLFGCFLNLGSSLTAEIVGMAGFDWALIDLEH
                     GAGFESHVLHQLQALEHTAAAALVRVESSQRQRLHRVLDLGAHGIMVPRVSNKEEALA
                     AVRGLRYPPQGVRGVASMNRACEFGARFRTYVLEANASLLGIVQIETEESLHNLDAIA
                     ETDGVDVLFLGPLDLSHGLGVAGQFDHPRFLEAVQATVAAAQRHGKAAGTLIGRPEDL
                     LKYTSLGYRFIGCGSDGGLLNSAARVTVAALRSAAKRG"
     gene            1602..>2067
                     /locus_tag="VFA54_09515"
     CDS             1602..>2067
                     /locus_tag="VFA54_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZS51086.1"
                     /translation="MSAKVETLAQLFVCGMEHDSGRRLFAREDGVYVPLSSAEFYRRV
                     ARLHIALRGLGFRPGDCCVLFSENRWEWVVADFAVLTSGMVSAAADPSLSAEQVYAVL
                     KGTAARAAVVSGREQREKVLAMWGDLPALDSIIICDGLTGGPKTRSFTISQLI"
BASE COUNT          370 a          584 c          674 g          439 t
ORIGIN      
        1 cttcgacgcg ctcgccatgc gcttccggga gctgaaaatc cgcaagaacc cggactgccc
       61 ggtttgcggc gaacaccgga cgattcacca cttgatcgat taccaggagt tctgcggcat
      121 tccgcatcac gagccgccgc ccgcgcccgg actgaaggaa tgggaaatcg atccggtcga
      181 ggtgaaagcc aagatggatc ggcatgagcc atttgtgctc atcgacgttc gcgagcccca
      241 cgaataccag atctgctcga ttccaggcag ccggttaatc ccgctcggcg aattgcccaa
      301 gcgggtccat gagctgaatt cggccgatga gatcgtggcg cactgcaaga gcggcatgcg
      361 cagcgccaaa gcggtggatt tcctgcgcaa ggccggattc aagaaagcga agaacatggt
      421 gggcggcatt ctggcctgga gcgacaaagt cgatccgcgc gtgccgaaat actgaaccgg
      481 caatctcgtc tagagcttcg gctggaagcg cccgatcggc ggccagccgg gtggagacgg
      541 cgtggtatcg ctgggaagcg gccggatatc cgctgtgtaa tccctctttt tccagagctc
      601 ccagggatat agggaatatt accggttaaa gtgaaaaatc ttcgaaaacg tgtgctggcg
      661 ggcgagacgc tgtttggctg cttcttgaat ctcgggtcgt cgctgacggc cgagattgtc
      721 gggatggcag gatttgattg ggcgctgatc gacctggagc atggagccgg cttcgagagc
      781 catgtgttgc accagctcca ggcgctggaa cacacggcgg ccgccgctct ggtgcgggtc
      841 gagtcgtccc aacgccagcg gctgcatcgc gttctcgatt tgggcgcaca cggcatcatg
      901 gtcccccgcg tgagcaacaa ggaagaggcg ctggcggccg tgagaggctt gcgctatcct
      961 ccgcaaggtg ttcggggcgt ggcgtccatg aaccgcgctt gtgaattcgg ggcccgcttt
     1021 cgaacttatg tgttggaagc caacgcctcg ctgcttggca tcgtacagat cgagacagaa
     1081 gaaagcctgc acaatctgga tgctatcgcc gaaaccgacg gcgtggacgt cttgttcctc
     1141 gggccccttg acctctctca cggtcttggc gttgccggcc agttcgacca ccctcgattt
     1201 ctggaagcgg tgcaagccac ggtggcggcc gcccagcgtc atggaaaagc cgcaggcaca
     1261 ttgattgggc gcccggaaga tctcctcaaa tatacgtcac tgggctatcg cttcatcggc
     1321 tgcggttcgg atggggggct gctgaactcg gcggcccgcg tgacggttgc ggcgctgcgc
     1381 tcggctgcca agcggggctg acgcgcctca gttcctcgct cattctttat caatttcaag
     1441 ggattggcga tttgtgatgt tgcgcacgaa agcggcggcg acctgtgcca aggagttagg
     1501 ttttagcggc gttccggtca tatccggtca ggtactgcta gttcattccc ggtactctca
     1561 gttcttttct ttcccctgcg caaggcgtat attaccttgg catgtccgcc aaggtcgaga
     1621 cgctggctca gctgttcgtg tgcggcatgg agcacgacag cgggcggcgc ctcttcgcca
     1681 gggaggatgg cgtatacgtt cccctgtcat ccgcagagtt ttaccggcgg gtggcccggc
     1741 tgcacatcgc gttgcgcggc ttaggctttc gtccagggga ttgctgtgtt ctgttttccg
     1801 aaaaccgctg ggagtgggta gtggcggatt tcgcggtgct gacctcgggc atggtcagtg
     1861 cggctgccga tccctcgttg agcgccgagc aggtgtacgc cgtcctcaag ggcacggcgg
     1921 cgcgcgctgc tgttgtttcc ggccgggagc agagggagaa ggtgctggcg atgtggggcg
     1981 atttgccggc gctcgacagc atcataatct gcgatgggct gaccggcggc ccgaagaccc
     2041 ggagttttac tatttctcag ttgatcg
//
LOCUS       DASREK010000185         4446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_919345, whole genome shotgun sequence.
ACCESSION   DASREK010000185 DASREK010000000
VERSION     DASREK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919345"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFA54_09520"
     CDS             complement(<1..648)
                     /locus_tag="VFA54_09520"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HZS51087.1"
                     /translation="MPETTEPRRQIVNFILYKLDPAWRLLPEEQKQRDREELCRVITS
                     WSHPGRMLILPYSTVGLRADSDIMLWRICYQLEDLQQMSTEISQTGLGKYLHTSHSFL
                     SMTKRSTYVLEHVHEGQADSRGTLVPGKYKYIFVYPFIKTREWYLLNLPTRQGMMNEH
                     ITVGHKYPSVKLNTTYSFGLDDQDFVVAFESDHPEDFVDLVMELRETDASKYTVRD"
     gene            complement(1266..2552)
                     /locus_tag="VFA54_09525"
     CDS             complement(1266..2552)
                     /locus_tag="VFA54_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51088.1"
                     /translation="MARRVTFALILALLLLGLLAPSAAASASSGVSSNSQSRDREGST
                     QAPETERSRLAVQMLEQTNLAREAIQKGAAQAARDHLKRAMGLAEALQSNAQVQADGN
                     LVPVYNQLEQASVIAPVLHWQAGPQGAPATGAADPAEGESAGRPVPPDAAGSASARRP
                     GSSLAERARSGGEVIRPTGPSTMVAVDVTLAKNHLEAAATALENRDLSRADAALVFVE
                     NGIFVIPAQLNPALVQAREHLSAARSHALDGDYDAATQPLKAAAQALSSYQNGRQKDA
                     ARSVQRQISAYLENRPKNHAHEVTRIDSWWDQVTGWMLQPAQSRGDAAGMNPSGTNTE
                     FASRPGAEARGTAHPSTIPGAAHSGAGRPTANQPGGTSPSPYTAQPGAAFPGATPGTR
                     GGTAPGVSQPSGASQPNIPGPSGLPNGPASPGTPLR"
     gene            2745..3197
                     /locus_tag="VFA54_09530"
     CDS             2745..3197
                     /locus_tag="VFA54_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZS51089.1"
                     /translation="MDTTGVDISDSEYRALAEFRHQIRRFLHFSEKAARAAGLEPQHH
                     QLLLALKGLPEGSVPSIRQIAERLQIQHHSAVELLDRLQERGLAERTRSSEDHREVLV
                     RLTERGEKILRELSLHHRAELQSAGPALVRALEQLTVRTPAAQEAACR"
     gene            3188..>4446
                     /locus_tag="VFA54_09535"
     CDS             3188..>4446
                     /locus_tag="VFA54_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZS51090.1"
                     /translation="MPVSAGESKLASVESFEPSGKLGDFTTSIRVLPLSLVAIAIGVL
                     SAYVALALLRLIGLFTNLFFYQRWATNLVSPADHHLGPFVIVVPIIGALIVGFMARYG
                     SERIRGHGIPEAIEAILINGSRVEPKVAFLKPLSSAISIGSGGPFGAEGPIIMTGGAF
                     GSMIAQFLHLTSAERKTLLVAGAAGGMSATFAAPVAAVLLGVELLLFEWKPRSLIPVA
                     LASATAAAVRRHLMGLGPIFPAPPHPAFVGPGGLLGCAASGLLAGALSVLVTLAVYAS
                     EDAFHKLPIHWMWWPAIGGLVIGLGGLIFPQALGVGYDTIADLLQGNVAKQVILGILL
                     VKSIIWAVSLGSGTSGGVLAPLLMMGGALGGLEAMVLPNEGTGFWPLVSMGAILGGTM
                     RAPFTGVIFAVELTHDLNALLPLLIAC"
BASE COUNT          752 a         1361 c         1402 g          931 t
ORIGIN      
        1 atcgcggacc gtgtatttgc tggcgtccgt ctctcgcagc tccatcacca ggtccacaaa
       61 gtcctctgga tggtcgcttt caaacgccac cacaaaatcc tgatcgtcga gaccaaaaga
      121 atacgtcgtg ttcaacttca cgctgggata tttgtggccg acggtgatgt gctcgttcat
      181 catgccctgg cgcgtgggga ggttcagcaa gtaccattcc cgggtcttta tgaaaggata
      241 tacgaaaatg tatttgtact tgcccggaac gagcgttccg cgggaatctg cttgtccttc
      301 gtgcacgtgc tcgagcacgt acgtggagcg cttggtcatg gagaggaagc tgtggctggt
      361 gtgcaggtat ttccccaagc cggtctgcga aatttcggtg gacatttgtt gcaggtcctc
      421 gagctgatag cagatacgcc acagcatgat atcgctgtcg gcgcggagac ccacggtcga
      481 ataaggaaga atcagcatgc ggccgggatg actccacgag gtgatgacgc ggcaaagctc
      541 ttcccggtcc cgctgctttt gctcttccgg cagcagccgc caggccggat cgagtttgta
      601 gagaatgaaa ttgactatct ggcggcgtgg ttccgtggtt tcaggcatag tggattctcc
      661 agtcagcgaa gccgtgccgc accgcctggc tgaaatggcg gcggcttcca gatctgcggc
      721 ttttctttcc caggcggccc gcgggatgta ccgctttaga aaatcatcat agcacgcggc
      781 tcgcggcgct cattccatct ccatcgcggc cgggaatgaa cgcaacaccg caagcgcggc
      841 gggccggttc cgggacctgg gcccgaagcc ggcctcgatg tggatctgaa tcggagccgc
      901 attggcggga tgcgcggcgc ggcatccgcc gcgaaatgag tactccacgt ttcgattgga
      961 cattttgaaa acgcaagttg aacgccgcca gcgccgaaaa cccggctccg cagagatccg
     1021 ccgacgagcg aagaagggaa acgctcccgc gcgagacggc tcgtccggct tgctgtttgt
     1081 gcccggagga tctctatttc agcgatccgt tctccgcgga tgagcgagag cgggaggtgc
     1141 ttctctgccg cggattccct cgcggatgca gcaaagtaga caggcctcat cgcctagggc
     1201 gaaccgggat ctcctccgcg aaaaaacggt ctcatccgct ttctgttcgt ttgtgggcgt
     1261 tcgcgctagc gcagtggcgt gcccgggctc gccggcccat tcgggaggcc ggatggtccg
     1321 ggaatattcg gctggcttgc gccgctgggc tgactcacac cgggcgcggt tccaccgcgg
     1381 gtgcccggag tggcgccagg aaaagcggcc cccggctggg ccgtataagg gctcgggctt
     1441 gtgccgcccg gctggttggc ggtgggtcgc cctgcgccgg aatgtgccgc gcccggtata
     1501 gtgctgggat gcgccgtgcc ccgcgcttct gcacccgggc ggctcgcaaa ttctgtgttt
     1561 gtgccggaag ggttcatccc agcggcgtcc ccgcgggact gcgcgggctg caacatccag
     1621 cccgttacct gatcccacca gctatcgatt cgggtcacct cgtgggcatg attcttcggc
     1681 cggttctcga ggtaagcgga aatctgccgc tgcacgcttc tcgctgcatc cttctgccgc
     1741 ccgttttgat aactggacaa agcttgcgcg gcagccttta gaggttgcgt ggccgcgtca
     1801 tagtcgccgt cgagagcatg cgatcgggcg gccgacagat gctctcgcgc ttgcaccagc
     1861 gctgggttta gctgcgccgg aatcacaaag attccgttct ccacgaaaac cagagcggca
     1921 tctgcgcggc tcaggtcccg gttttccaat gccgtagccg cggcctccaa atggtttttg
     1981 gcgagcgtca catccaccgc caccatagtg gagggcccgg tcggccggat cacttcgccg
     2041 ccgctgcggg cccgctccgc caggctgctg cccggccggc gggccgaagc gctgcccgct
     2101 gcatccggtg gaacaggacg accggcgctt tccccttccg cagggtccgc cgcgccggta
     2161 gccggcgcac cctgtggccc tgcctgccag tgcagaaccg gcgcgataac ggacgcctgc
     2221 tcaagctggt tatacaccgg gacgagattc ccatccgcct gaacctgagc gttcgattgc
     2281 agcgcctccg cgagacccat agcccgcttc agatgatcgc gcgcggcctg cgccgcgccc
     2341 ttttggatag cttcgcgcgc cagattggtt tgctcgagca tttgcacagc cagccgggag
     2401 cgctctgttt ccggcgcctg tgtggaaccc tcccgatctc ggctttgcga attggatgaa
     2461 acgccagaag aggcgctcgc ggcggcggac ggcgcgagca aaccaagcag cagcaaagcc
     2521 aggatgagtg cgaaggtcac ccttcttgcc atcggcattc gctcctcctc ttcgtagcaa
     2581 ttccactaaa ttggacgtct gccgcgtcct ccaagttggg gcgcgaacat cccttcggcg
     2641 gcgacggttg tccaggatgg tgttgcaccg cgccaaatag cctccctgag cggcgcagaa
     2701 accaagcaga gcttttcgta tcgcaatacg atataatttt aaacatggac acaacagggg
     2761 ttgatatatc ggattccgag tatcgagctt tggcggagtt ccgccatcag atccggcgct
     2821 ttctccattt cagcgaaaag gcggcgcggg cggctggcct cgagccgcag caccaccagt
     2881 tgctattggc tctgaagggt cttccggagg gaagcgttcc ttcgatccgc cagatcgctg
     2941 agcgcctcca aatccagcat cacagcgctg tggagctgct cgaccgtttg caggaacggg
     3001 gcctcgccga acggacccgc agttcggaag atcaccggga agttttagtt cgcctgacgg
     3061 aacgcggaga aaagattctt cgggagctct cgctgcacca ccgggctgaa ttgcaatcgg
     3121 ctggccccgc cttggtgcgc gccctcgagc aactcacggt ccggactccc gccgctcagg
     3181 aggccgcatg ccggtgagcg cgggcgaaag caaactcgct tctgtggaga gcttcgagcc
     3241 atccggcaaa ctgggcgatt tcaccacaag catccgggtg ctgcccctgt cgctggtcgc
     3301 gatcgccatc ggcgtgttga gcgcatacgt ggcccttgcg ctgttgcggc tgatcggcct
     3361 gtttaccaat ctcttttttt accagcgatg ggctaccaac ctggtttcgc cggcggatca
     3421 ccatttgggc ccgtttgtga ttgtggtccc gattatcgga gcgctgatcg tcggattcat
     3481 ggcgcgctac ggatcggaac gcattcgagg tcatggcatt ccggaagcga ttgaagcgat
     3541 tctgatcaat ggaagccgcg tcgagccgaa ggtagcgttt ctaaagccgc tttcctcggc
     3601 gatttccatc ggatcgggtg ggcccttcgg ggccgaaggc cccatcatca tgacgggcgg
     3661 cgcctttggc tcgatgatcg cccagttttt gcacctcacc agcgccgagc gcaagacgct
     3721 gttggtggcg ggcgcagccg gaggcatgtc ggcgacgttc gctgctccag tcgcggccgt
     3781 tttgctgggc gtcgaactgt tactgttcga atggaagcct cgcagcctga ttcccgtggc
     3841 tttggccagt gctacggcgg cggcggtgcg gcgccatttg atgggtttag gcccgatttt
     3901 tcctgcgccg ccgcatccgg cttttgtcgg tcccggggga ttgctgggat gcgccgcatc
     3961 cggcttgctg gctggcgcgc tctccgttct ggtcaccctg gctgtgtacg cctccgagga
     4021 cgcattccac aagctgccaa tccactggat gtggtggccg gccatcggag gcttggtgat
     4081 cggcctcggc ggcctgatct tcccgcaggc gctcggcgtc ggctacgaca caatcgccga
     4141 cttgctgcaa ggcaatgttg caaagcaggt catattgggc attctgctgg tgaagtcgat
     4201 catctgggct gtatcgctgg gatcgggaac ctcgggcggc gtgctcgcgc cgctgttgat
     4261 gatgggcgga gcgctgggcg gcctcgaagc tatggtgctg ccgaacgaag ggaccgggtt
     4321 ctggcctctg gtcagcatgg gagccattct gggcggcaca atgcgcgccc cattcaccgg
     4381 cgtgattttc gccgtcgagc tcactcacga tttgaacgcc ctgctgccgc tgttgatcgc
     4441 ttgcat
//
LOCUS       DASREK010000186         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1740624, whole genome shotgun sequence.
ACCESSION   DASREK010000186 DASREK010000000
VERSION     DASREK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740624"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /gene="yidC"
                     /locus_tag="VFA54_09540"
     CDS             complement(<1..981)
                     /gene="yidC"
                     /locus_tag="VFA54_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZS51091.1"
                     /translation="MPEYKNPQTEPGMDRNLLLVFLVLAVAIFGSQLFMRKYAPPASP
                     PTSAKPAQVTPPASGNPPVSAATPAPAPAPATRVPVPSVSKQAASESETVVENDLYRI
                     TFTNRGAQAKSWVLKKYQDDQGKPLDLVNSAAAAKFGYPLSLWTYDESLRNKLNSALY
                     VSSAASQNLSTPAEIVFDYADSGLVVHKAFQFDDSYVVSVTTSVLSGGSPVYGFPAWP
                     AGFGDQVSLAAYGAGQLEYQFNNDTEHLGLKKVAGGATLHGSYDWAGVSSQYFTAVFI
                     PNTPENVQMVTLHYPLDIAPDPGKPNETRPADVLGVAVGRPGESVERLFVG"
     gene            complement(994..1332)
                     /gene="yidD"
                     /locus_tag="VFA54_09545"
     CDS             complement(994..1332)
                     /gene="yidD"
                     /locus_tag="VFA54_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZS51092.1"
                     /translation="MKRHQPASSGLRVLSFAQTSLLGLFRVYKRALSPFLPVACRYVP
                     TCSEYAAEAVERHGVLRGSALAAGRVLRCHPFVRGGFDPVPPKNVPSDALNRGAGGAR
                     TSPRLHGSIS"
     gene            complement(1329..1730)
                     /gene="rnpA"
                     /locus_tag="VFA54_09550"
     CDS             complement(1329..1730)
                     /gene="rnpA"
                     /locus_tag="VFA54_09550"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZS51093.1"
                     /translation="MSHPVPQNAKFAKSRRLLRHADFERVYKQGRRHFAAHMTVFYLR
                     RAEGESGDSRVGLTVGRILGGAVQRNRIKRRLREAVRLSRPGFSAPVDMVINPKRSAL
                     NMDFVDLRQELTTAFQVIERSLEKEKGNPRG"
     gene            complement(1802..1957)
                     /gene="rpmH"
                     /locus_tag="VFA54_09555"
     CDS             complement(1802..1957)
                     /gene="rpmH"
                     /locus_tag="VFA54_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZS51094.1"
                     /translation="MPKRTFQPNRRRRSKTHGFRVRMKTKQGRAVLSRRRAKGRKRVS
                     VKPGFRE"
     gene            2503..>3129
                     /locus_tag="VFA54_09560"
     CDS             2503..>3129
                     /locus_tag="VFA54_09560"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HZS51095.1"
                     /translation="MRARAFTQAKLRSAVSGAGALAEGLFAALFPSDCRLCGLPLTKI
                     SRLPVCTNCLAAMGPIAGETCGICGETVSSQVFAVGVAPCPACQESRPSFTRAVAYGS
                     YDGELRELIHLLKYDGVVPAAKVLGRMLAQAIQKLDLAPGPLLVIPVPLHRSKKRQRG
                     FNQAELIARKALRRLSLPDAQLASGILARSRTTASQIGLTRPQRAENIR"
BASE COUNT          581 a          954 c          980 g          614 t
ORIGIN      
        1 cccgacgaac agccgctcca cagactctcc cggcctgccc acagcgacgc cgagaacatc
       61 cgcaggcctg gtttcattgg gcttgccggg atcgggcgca atgtcgagag ggtagtgcag
      121 ggtgaccatc tgcacatttt ccggagtgtt gggaatgaag accgcggtga aatattggct
      181 gctgacgccc gcccagtcgt atgaaccgtg aagcgtcgcg cctcccgcga ctttcttcag
      241 gccgaggtgc tcggtgtcgt tattgaactg atattcgagc tggcccgcgc cataggcggc
      301 caggctgact tgatcgccga atccggcggg ccacgccgga aagccataaa ccggactgcc
      361 tcctgacagc accgaggtgg tcacgctcac gacgtagcta tcatcgaact ggaaggcctt
      421 gtgcacgacg agaccggaat cggcatagtc gaacacaatc tccgcgggtg tggagagatt
      481 ctgcgaagcc gcgctggaga cgtacagggc cgaattcaac ttgttccgga gagattcgtc
      541 gtaagtccag agcgagagcg gatagccgaa cttggcggcg gccgcggaat tcaccagatc
      601 gagcggcttg ccctgatcgt cctgatactt tttcagcacc cacgacttcg cctgtgcgcc
      661 tcggttcgta aaggtgatgc ggtaaaggtc gttctcgacg acggtttcgg attcggaagc
      721 ggcctgcttc gaaactgacg gcacgggaac acgcgtggcc ggcgccggag cgggcgcggg
      781 cgtggccgca gagactggtg gatttccgga ggctggcgga gttacctgcg caggttttgc
      841 cgaagtggga ggcgaagccg gcggggcgta ctttcgcata aagagctgcg atccgaagat
      901 cgccaccgcc aggaccagaa acaccagcag caggttgcgg tccatgccag gttcggtttg
      961 tgggttcttg tactcaggca tcgcgatcag cgctcacgat atcgaaccat gcaggcgcgg
     1021 actcgtacgt gcgccccctg cgccgcggtt gagggcatcc gagggtacat tcttcggtgg
     1081 caccgggtca aaacctccgc gcacaaaggg gtgacagcgc aaaacgcgtc cggccgccag
     1141 ggccgatccg cgtagaacgc catgccgttc cacagcttcg gcagcgtatt ccgagcaggt
     1201 cggcacgtaa cggcaagcca ctggaagaaa cggcgagagc gcccgcttgt aaacgcgaaa
     1261 aaggcccagc agtgaggttt gggcaaacga caaaactcgg agcccggagc ttgcaggctg
     1321 atggcgtttc atccgcgcgg gttccctttc tctttctcca gcgatctctc gatcacctgg
     1381 aaggccgtgg tgagctcctg ccggagatcc acaaaatcca tattcagagc cgaccgcttg
     1441 ggattaatga ccatgtccac cggtgcggag aagcccggcc ggcttaaccg gacagcttcc
     1501 cgcagtcgcc gcttgatgcg attgcgctga accgcccctc ccaggatcct ccccacagtc
     1561 agcccaaccc ggctgtcgcc agattcgccc tcggcacggc ggaggtagaa cacggtcatg
     1621 tgcgccgcga agtgccgccg cccctgtttg taaacccgct caaagtccgc atgccgcaat
     1681 agccggcggc tcttcgcaaa cttcgcgttc tgcgggacag gatggctcac aggccgactc
     1741 caggctttag gcgccgggct tcaggagttc gaccgcttct gcctgaagcc tgaagctgcc
     1801 tctattcgcg aaatccaggc tttacggaaa cccgcttgcg ccccttggcc cggcgccgcg
     1861 aaaggaccgc gcgcccctgc tttgtcttca tgcggacgcg aaagccgtgc gtcttcgaac
     1921 ggcggcggcg gttgggctgg aatgtacgtt ttggcatgaa aaatcctgcg ctcctgtcct
     1981 ctgcaatacg gttgaatgaa aggaaagtat aaacgagcgc cgggaaaaac ggtaagcgcc
     2041 gccggggtac tgggcgtttc tcgccgcggt gcttgctcgg gctgctagaa tcaggacaag
     2101 ccacttggtt ggaaattcca gaaagtcggg gaggtagtgt cgtgttttcc agccagttcc
     2161 gctgcccgga ctgcggcggg tccgctgctt atcgttcccg gcgtcgcagt gttttcgaga
     2221 gatatgtgct cccgtttctg ttgctgcgtc cggtgcgttg cgccaattgt ttccggcgca
     2281 gcagcgtctc ggtgtttgta gttgcacgcc cgcgcgagac ccgctccgcg atcaagcccc
     2341 gtgccgctta gcgaattgct tgcgtctccg gctgccgcta tactggatgc aattcgtccg
     2401 gctggagcct aaccaggcgc cgtacgaggg aggctggtct tcagcagtat cggagacata
     2461 actcgcacca cggtacccgc tccgcctcag aacgcctttt tcgtgcgtgc cagggcattt
     2521 acccaagcca agctgaggtc tgcggtttcc ggagccggcg ctctcgccga aggcctcttc
     2581 gctgcgcttt tcccctccga ttgccgtctc tgcggcttgc ctctcacgaa gatttccagg
     2641 ctcccggtat gcacgaattg cctcgccgcc atgggaccga tcgcgggaga aacctgtgga
     2701 atctgcgggg agacggtctc ctcgcaggtt tttgccgtgg gagtcgcgcc gtgcccggcg
     2761 tgccaggaga gccgcccgtc cttcacccgg gccgtcgctt acggttccta tgacggtgag
     2821 cttcgcgagt tgatccatct tttgaaatac gacggggtgg tcccggccgc gaaagttctc
     2881 gggagaatgc tggcgcaggc gattcagaag ctggatctcg cgccgggacc gctactggtc
     2941 attccggttc cgttgcatcg gtccaaaaaa cggcagcgag ggttcaatca ggctgagctg
     3001 attgcgcgca aggcgttgag gcggctgagt ctgccggatg cacagcttgc gtccggcatt
     3061 ctggcgcgct ccaggacgac agcatcgcag atcggcctga cacggccgca gcgcgcagaa
     3121 aacatacgc
//
LOCUS       DASREK010000187        44065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_425407, whole genome shotgun sequence.
ACCESSION   DASREK010000187 DASREK010000000
VERSION     DASREK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 44065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44065
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425407"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VFA54_09565"
     CDS             <1..793
                     /locus_tag="VFA54_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS51096.1"
                     /translation="SGKIKGRIPDLPIVTIASAQAALRVSAPDAAPDSWQPVVRREDR
                     ERWNDWGIGLLLQGDLKGAEYAFQKVTEAEPSYADGWLNVARAYIQEGQTDAAKPYIE
                     KALSLNPHLGRIYYFRALVEKADGDYDAALRSLRAAAAQYPRDRVVLNQIARILFLKR
                     DYRGALAILDRVCAVDPEDLAMHYTKMLCHRGLGEVDAAAREERLFRRFKADEASQAI
                     TERPRRTSPEDNNERQPIHEHESAPIAKAVRRIEPAPAVAAGGGS"
     gene            790..2463
                     /locus_tag="VFA54_09570"
     CDS             790..2463
                     /locus_tag="VFA54_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZS51097.1"
                     /translation="MKPPRMGLGAFLAACCVVIAAAPAGVQFTDVTTTAGIRFRHNTG
                     RSGKKYLPETLGSGCAFLDFNNDNYADILLLNGKDWNPAASAARHSYPALYRNNGNGS
                     FTDVTAAAGLAIEMHAMGVAVGDYDNDGWQDLYITALDGDRLLHNEHNGTFRDVTRAA
                     GINNAGFGTSAAWFDYDRDGQLDVFVANYVQWSPQTDLWCSLDGRTKSYCTPESYKGL
                     PAKLYHNLGGGKFEDVSRKAGIADPAGKSLGVAVLDFDGDGWPDLFVANDTQPNKLYR
                     NNRDGTFTDIGVAAGVAFGEDGVARGAMGVDAADYDRSGRPHLLVGNFSNQMLALYHN
                     EGKALFVDEAPASAVGRASLLSLTFGLFFFDYDLDGHPDIFTANGHIEDEINRVQPKV
                     HYEQPPLLFHNLGGRRFELATDQVGPFLRRPIVARGAAYADYDLDGDLDILITTNGGP
                     AYLYRNDGGNRNNFLRIKTRGSRSNRDGIGAVVRLRSASGSQWQSVHSGSSYCSQSEL
                     PLTFGLGRDARVDLLEIDWPSGAHQVLSNLPVNQFILIQEDQQSVSRIR"
     gene            2699..4012
                     /locus_tag="VFA54_09575"
     CDS             2699..4012
                     /locus_tag="VFA54_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS51098.1"
                     /translation="MATAFQQHDPAGGIPPFRLDVTLHDLLRKQAPRPPAPLPAAEPQ
                     KLPDPVFPQHPELLGDHGDYVPAHRPGASPYRRRWTAPLVYRAMRGWLFPYFKSRILP
                     GDFHPIIAYLFTEWKCNLDCYYCWAFDNHVKGMSEDTAKRSIDWLHTTTCRVLALMGG
                     EPLLRPQFVHKVVYYAAKKGFWVYLPTNARLLRPDVIDRIADAGVAIVNFAVDVVDEK
                     PGLPKALNPVRKYFDYLIRKQYKYGYCVFFNINICRNNLEDVKQLTEIAHDNGIATDY
                     HINESPMIEQDHFQHLEENDTYIRPEDWPKVDGLIDWIIEKNRAGYKMVNSVRRLQDM
                     RDFMRGKVEPWNCRAGQNSLIIRVDGTLAPCFPMYSATYDWGAIENPKFEVKQLNEMK
                     QSCQTHCFSTLNHNLGYCYNDGRVIKWVLRQAARGFQGVSGSFYD"
     gene            4192..5337
                     /locus_tag="VFA54_09580"
     CDS             4192..5337
                     /locus_tag="VFA54_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51099.1"
                     /translation="MRIVSTLLAAALFTIAGFSQENSGGLDPAVRGLLRPESGFLLGV
                     QWRKALSLLPADILKKQLAVSGLAASADAADAESVILEDIDAVYVAGSTARDVAAGRQ
                     TPVLAIVRGRFDRTRMQGLLRGHGEIYRRVLLMTPPGVSPPQTRVAMLDEGTILIGDR
                     KEMMAALDRLAAPSAPQPAGKTRTTLLDRAEVLAKQNDLWLLIDAPPGGFQPPPPQAR
                     GSGTSAAFVPSPEILSNLRGLDLGVSFSGGLRLQVGLRAKSAEAGKNLAAAVQGLLAL
                     AALSQQAKPEALDALRRIEIKPQASTVNVSLSLTEQEVRKVYAEMQASAKNASARRFG
                     GPAVSGGNVAVSGADRPSPAGKTPRRGTIRILGQDTGTVEVPVGGQK"
     gene            complement(5507..6100)
                     /locus_tag="VFA54_09585"
     CDS             complement(5507..6100)
                     /locus_tag="VFA54_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51100.1"
                     /translation="MPFPEKIPVKISSEAAGYVSITPVVAEQMRAPDLIEHILRVAGK
                     DVARAQEILRQGIVVSGASRIRWSPIEASSEEIAGAFAAFPDPEPGRPFDASKCVRAA
                     LEGGRAVIELPREAASRKRWFRRQSFWETLMETASRLPLKYQQYSYGDRADVYRGELP
                     LEPARILRERAWMLRYASLEMQVREYAYDRIGLWVAR"
     gene            complement(6104..6292)
                     /gene="rpmB"
                     /locus_tag="VFA54_09590"
     CDS             complement(6104..6292)
                     /gene="rpmB"
                     /locus_tag="VFA54_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZS51101.1"
                     /translation="MAQACELCGKKPMTGHRISHAHNLTKRRWLPNLQRVHAVVGGAH
                     KTIRVCVSCIRSGKVKKA"
     gene            6414..7667
                     /locus_tag="VFA54_09595"
     CDS             6414..7667
                     /locus_tag="VFA54_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS51102.1"
                     /translation="MRWRELFAETLRALAANKMRSFLTMFGIAWGILSITLMIAAGEG
                     LKMGQAAAQRTLGKDILIAFPGRTSMQAGGLRAGRRIFFQLSDLDAVRRDCPDVAQLT
                     PELGRPGVYVRSAYNSGSFRVTGALPFFQDIRSLDVAKGRFWTYADEQMRLRVAVLGD
                     EVRKQLFASRNAVGETIQVGDFPYQVIGVLARKDQDSNYDGPDNQKIFVPLAAVSTDF
                     PSLPPAAPNSVDRVVATPVSVSRHEEAEAELRRAFARLHQFDPADKDAVFVWNTIKSA
                     RIFSRVFDSMELFMGVVGVITLLLGGIGVMNIMLVSITERTREIGLRKALGATRRVVL
                     IQFFSEALLLTLVSGGAGLAIGYGICLAVNRLPMPQYFAGLMVTPWVGLFASLVLTLV
                     GVASGLYPAKVAADMTPIEALRYER"
     gene            7726..8973
                     /locus_tag="VFA54_09600"
     CDS             7726..8973
                     /locus_tag="VFA54_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS51103.1"
                     /translation="MELIRQAAMALRRNIFRSCLTVLGIVWGITAVSMLQSYGDGFRQ
                     AVVRAFDAFGPDVIMVWPGQTSQQAGGERAGRKIRLELEDVRRIREDGTLIRSASPEL
                     FKPAQAVNGVRQASVGVRAVHPCYGEIRNETPAEGRFISDEDLAQRRRVVFLGSEARR
                     KLFGNIDPIGQAILLNNARFIVIGWMEKKLSFSSYFRSDDESVFIPLTAGADLWDTRY
                     LSVIVAEPVARSFSAAAVRQLREILARRQRFSPRDERAVQIFSREQVRPVIDGITIGI
                     ELLLIVVGALTLGIGGVGVMNIMLVSVQERTREIGVRMAIGATRRQIRIQFLLEALVI
                     VAAGGLIGLGVSYVVVHFVGVLPLLGQTWNDDSGRADIHLQMSWITTLRSIGLLAVVG
                     LLSGIFPAIKASRLNPIEALRYE"
     gene            complement(9073..10002)
                     /locus_tag="VFA54_09605"
     CDS             complement(9073..10002)
                     /locus_tag="VFA54_09605"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HZS51104.1"
                     /translation="MTESDSLNYRPALSASFFRPRWKTAIEWAAALAMAALWLSAGLW
                     KLSDLSGWQVKLTQMLVPHGISMAGAVFFGAAETFAGILLLAPQWRRWGAWLSVFLLS
                     AFMIYIGVHYRALTGADCSCFPWLKRAIGPMFFVEDGAMMAVAVLAGAMAGRSRGGGK
                     ASAMLGLVLLLAGASVAMDRVRGQGVTGPESITAGGKQFSLREGRVFLFFFNPYCPHC
                     FQAAQAMSVLSWQAAIIAVPTQAMNDAPGFLQSSGLTSALLSPDLEKLKQSFPFQDTP
                     YAVALENGRLVDKVTFFDEPDLGKQLRKAGFIK"
     gene            10057..10626
                     /locus_tag="VFA54_09610"
     CDS             10057..10626
                     /locus_tag="VFA54_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51105.1"
                     /translation="MVRRSCAGWVSFLAWLTSFATAGAAAQHRETFNYSFDVDSGESR
                     YFEIPTKEAGARLRVEFEVRSPQRFPGIRVAVEKQDQFERLRQHQPHQDLAFLSYRRE
                     GTLQVQLPESGRYAVVINSPPETHRRCRVEMDVTLTTGPDPETLPVSYASPRTRLIVV
                     TASLGGFVLILLLSGRALWRAARRSLRLF"
     gene            complement(10706..10858)
                     /locus_tag="VFA54_09615"
     CDS             complement(10706..10858)
                     /locus_tag="VFA54_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HZS51106.1"
                     /translation="MEENFKWLWYAFSAAWVLVVAYLFSLGRRESKLRDELESLKSMI
                     EERQRK"
     gene            complement(10871..11557)
                     /gene="ccsA"
                     /locus_tag="VFA54_09620"
     CDS             complement(10871..11557)
                     /gene="ccsA"
                     /locus_tag="VFA54_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZS51107.1"
                     /translation="MKNRSSAILAAVSGVMLVITLYLTFLWVPMERTMGVVQRILYFH
                     VPPFFVASLAILAGLVASIRFLITRDYRFDDLAVSAIEVGWVFDSINLVMGPFWARPV
                     WGIWWTWDARLTSAFVLWLMFAGYLILRRAVADPSARAVMCSVVVIFQAVGGVIVYMA
                     NRWWRTQHPQPVMAGGQGSGLDPRMWYVFLFSLATMLVLFGALLQIRRRLERLRREID
                     GLRRTVHSEA"
     gene            complement(11562..12239)
                     /locus_tag="VFA54_09625"
     CDS             complement(11562..12239)
                     /locus_tag="VFA54_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZS51108.1"
                     /translation="MDVLRGTLIIALKDLRVELRSKEVINAALSFALVILLLFSFAFD
                     PSAEETREIAGGLLWLVFIFAGILVLNRSFARETVNDCLDGLVASPLSGAPILLGKAV
                     SNTVLLLVLELVTLPIFGIFYNVRWTVGLGWLLLVLLLGTWGLTVVGTLFSALTVNVS
                     LREAMLPLLVFPITLPILMAAVQLTTTLFSGEPIAPDMIWLRVLIGCDIIFTVLAGAL
                     VESVLVG"
     gene            complement(12239..12970)
                     /locus_tag="VFA54_09630"
     CDS             complement(12239..12970)
                     /locus_tag="VFA54_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51109.1"
                     /translation="MRAAPLISAKGVWKFYGDFAALRDINLDVAPGVTLALLGRNGAG
                     KTTLLRMFAALSSPSRGSITISGAAAGAEETLRSPAQRRRIGVIGHSIWIYDDLTAAE
                     NLEFFARLYGPHAPHDKIDHWLETTGLDHVRNRQAREFSRGMRQRLAIARAFFHDPEI
                     LLLDEPFTALDDRAAALLSRLIEQAHASGRTAVISTHNLREALALATDVAILDRGRLA
                     FRGPSTAELRAAPEDFYRTYTEPRA"
     gene            complement(12967..14166)
                     /locus_tag="VFA54_09635"
     CDS             complement(12967..14166)
                     /locus_tag="VFA54_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS51110.1"
                     /translation="MKAASFNIQIRRKLLPLRQDEDMGAQRRRPGAAVLPLFRRGLVC
                     ALLLATPAAAAVEGVVINGTTGREQPGVEVTLIKLDQGMVPVGTTKSDAAGKFRFAVD
                     AEPGAPMLLRGDFEGVNYNQMIPPGSRAADVRLTVYRSAPARDTAGAPEQRIILLEPS
                     GGQMIVSEFYVYRNNAQPPVTYVDPKQGTLRFYLPPAAKGVVQVNATGPGGMPLKQTA
                     EKANQPDFYKVNFPIKPGETRLDITYLVPYHSPLEFEVRSAYDWLTTRVAAPSGVTLQ
                     GEGMEALPENPQIKASIFGLPEAKSYKMTISGEGRLSRGEEQGGEQNADSISISPAPI
                     NNKLWLVLGFAMGILALGFYALYNAGPGGAAEAAAQSAGKPQAMSMKNAGPSQQKSSR
                     ARSKRKA"
     gene            complement(14163..14603)
                     /locus_tag="VFA54_09640"
     CDS             complement(14163..14603)
                     /locus_tag="VFA54_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51111.1"
                     /translation="MIFAAAAVLIVAVLAFTLVVRQKDLPQPEPVSPTLHLEEKKKAI
                     YDNLRDLQFEYRMGKLSDEDYAATKLELQRELAAVLAEIDNVLKKTAQKPAEGAGTVR
                     PQEAGAARAHMKSPAKCVCPHCLAEFPQPMKFCGECGQAIAATS"
     gene            complement(14660..15244)
                     /locus_tag="VFA54_09645"
     CDS             complement(14660..15244)
                     /locus_tag="VFA54_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HZS51112.1"
                     /translation="MRFWRKDNEAAAWGLPGGRIKTPNAAAWIAGCVCAIVLLGVGLS
                     AQTVSTGDPMHKRLRAIGQKLQCQCEPCGKTVGDCDMGGCRYRDQVNSEIRPMLDSGM
                     DEAAILAKLREKFGTLILASPPAQGFSLLSWIMPFAALAAGLIVIRMVLKRWMRPEPA
                     ATVAAGSQTGAGAPSSLLLEKYRDEIEKELEEAE"
     gene            complement(15222..17237)
                     /locus_tag="VFA54_09650"
     CDS             complement(15222..17237)
                     /locus_tag="VFA54_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HZS51113.1"
                     /translation="MENLGSLALLLAFCLSIYTILAALVGKWKKKPYLSLSAERGVLA
                     IWGLVTSAAAILIYLLFANDFRLSFVAAHSNRDLPAIYKFAAWWGGQEGSLLFWLWIL
                     STYCVVSVYWNRRKHRDMIAYVIAILGGIETFFLVLVNFVANPFRVLTSAGQIMAVPD
                     GQGLNPLLQYWTMVIHPPMLYLGYVGFSVPFAFAMASLITRQKGDGWIGTTRIWTMVT
                     WLFQTCGICLGMRWAYAVLGWGGYWEWDPVENASLLPWITATAFLHSVMMQEKKGMMK
                     VWNMVLVSATFFLCIFGTMLTRSGLVSSVHAFAEGPIGKYFVTFLAIGIAGTIYLILN
                     RLDYLKSENQLESVVSRESSFLFNNLVLLGSCFAVLWGTLFPVISEWVTGSKISVGPP
                     FFNRVNVPIALFLLFLTGVGPLFAWRRTSVESLKRNFLWPSVAALALGALLLAAVTRN
                     FYALMSFMLCTFVGVTVLAEFYRGARAIQAKNGGSLLGCAVELTHRNTRRYGGYIVHL
                     GIVIMFIGFTGQVFSREAQDALQVGDTLQVAPYAFRIADIKQGENGNYAYETATIEVS
                     KNGQPLGKMYPERRLYRASQQLTSEVAIRPRLSEDLYISFAGRTNDGSKPVFQVFVNP
                     LVNWVWLGAFVVILGTLTALVPSKVKLSYARTQVIATAKTHEVLAER"
     gene            complement(17262..17696)
                     /locus_tag="VFA54_09655"
     CDS             complement(17262..17696)
                     /locus_tag="VFA54_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZS51114.1"
                     /translation="MRRYGKFVALVVIVLGTLVWLALAGINESKTYYITVSELQAMQD
                     KALDKRLRVAGDVEPNSIRRSADRVEFVLLQDNRRLKVVYTGRDPLPDTFKDRAQCLA
                     DGRLGRDGVFEAKRIQAKCASKYEAMPGGQPSANRQEPQRSS"
     gene            complement(17811..18860)
                     /locus_tag="VFA54_09660"
     CDS             complement(17811..18860)
                     /locus_tag="VFA54_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HZS51115.1"
                     /translation="MIKTTVVGSYPIPLWLNGNPSRAALRDAVMVVLKTQELAGLDVI
                     ASGELERFDPSHPETNGMIDYFVSRMSGVETRYSISDLAAFRADAGMGFRKAPAGIVR
                     GEPGEGSLNLPGDFEFASALTRSPLKFTCTGPHMLAKTLVDRHYRDPGRLSMALAAVL
                     RNQLAAVCSTATPPAVIQLDEANISGHPEDQTWAVEAINHVLDGIAVERAVHICFGNY
                     GGQSVQKGFWRNLLPFLNGLHAGHAVLEFARRGYEELAAFRDLDPKIGLGLGVVDIKD
                     NEIEAPDLIAARIEQAFEVLGEGRVKWVHPDCGFWMLPRNVADGKMRALVEGRNLFYG
                     RGIHNLFTAGPARAI"
     gene            19046..20236
                     /locus_tag="VFA54_09665"
     CDS             19046..20236
                     /locus_tag="VFA54_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HZS51116.1"
                     /translation="MSRILLLLLSAAGLAAGQQVQFRAHVIESKIPGGYSVIVVDVNH
                     DGKPDVIGMTQQVRQLKWYENPSWEPHVIIDDMTGMVNLAAYDIDGDGVPELAIESGF
                     SMVAAKSEGLVWLLQHQGDPKQLWKARRIDALTTSHHIAWADVDGDGKKELINAPLIG
                     ATNVAPKYEGKTPLVFYRVPKDWSSDWKRLMIDDQLNGITHRVRVVHWNGTGKREQLL
                     TAGFDGVILHSASGKGDKLQWEHKLLVKGHDTEEAPRLGTSDVKVAHLGKRRFLAAVE
                     PWHGNEVVVYTDDGKGGWKRQVIYDQLTEGHEVCVGDFNGDGRDDIVAGDRARGKVAS
                     SHIFYSLDDAGARWRHEELDHLGMSASGCQVADINGDGRPDIVMIGGATANIKWYENL
                     GPAK"
     gene            complement(20339..21457)
                     /locus_tag="VFA54_09670"
     CDS             complement(20339..21457)
                     /locus_tag="VFA54_09670"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HZS51117.1"
                     /translation="MKNAAGALGAVAAAPMINRGRFRLFAQEETTYSGRTIELVGRTT
                     VIDMLGLVTLDFPKLYAWQLAPETFQPPDFEKLQNSGITIFHPAVGHDEGDIYSASLL
                     DMRRWNNLIAKQSRYFLRIDRAGDFAVPKRQNKIGILLGEQNSEHFRSVDDVNRFFAL
                     GQRVSQLAYRTNRIGAGAFDPRDPGLSEFGRRIIERMNEVGMAIDVSHCGDRTTLDAF
                     EASRKPVLVTHSNCRALVPNRCRCKTDEAIQKMAAKGGVMGITMIRSFVRNGGPATMD
                     AVLDHIEWVVRLAGVEHVGVGSDVDLDGRSRLPPEIQRAQGIRPGEKIDLDGIDYEKK
                     IFDLTEGLVRRGYTDRQIELILGGNFQRALSEIWTIHT"
     gene            complement(21708..22052)
                     /locus_tag="VFA54_09675"
     CDS             complement(21708..22052)
                     /locus_tag="VFA54_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZS51118.1"
                     /translation="MAQVLVNWLLSAVSLLIVAHVIRGFEISGLGTALVAAIVIGFVN
                     ATLGFFLKIVTLPLTVVTLGVFWLIINALMLKVAAALVPGFAIRGFLPAFFGGIILSL
                     VNLGLRYLVAGV"
     gene            complement(22345..23790)
                     /locus_tag="VFA54_09680"
     CDS             complement(22345..23790)
                     /locus_tag="VFA54_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51119.1"
                     /translation="MYWHSVRDSRLIPPALLACVLLTAARLHWIYAQVFEILPDDILL
                     MGVSRKIAGDWSEIRKLLWTPQHIHVLPLFRLLRLPFDLHFPAWYAGFHAVVEAAHLA
                     SAALLYALARRYLRSPWAALATAASFAWSTAGDEALAWKAASPFALSWTFLLLGIWLL
                     TQNTGMSEWIAAASLLGSVGLFSGALFALPGVIAAVALLEPAAAARALRASGAAWTLG
                     AVSWLALVPRSANLRHYWRAGGASAGVLLRLAWAARDTLHSSIYQFGLAIRVLPGTYR
                     FSILFAVPALILAALWSWTNLRWVLASACLTLPPLFVIMLVRAEPEVWKISRYAYQSF
                     TLWAMFSGAVWDGLQRKWQKRPRLRLALALAAAALALCYLSSQLSVARHHRDELLAQP
                     INQPAFWFGWDAVFHAASQRSLQAGKPMRIPFVQAIPGLELTEVYRICEPRGLPGLFA
                     EHGVAGSAAERAEWERAIEQAHVPLPGWSAQ"
     gene            23868..24467
                     /locus_tag="VFA54_09685"
     CDS             23868..24467
                     /locus_tag="VFA54_09685"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZS51120.1"
                     /translation="MAADTAGYVLVGGRSSRFGSNKAFFSWQGRPLALRVAEQVRQAA
                     GSVVLIGDPETYRPLGLPVIPDPVSGAGPLAGLAAALGHSSAAWNLVVACDMPHLRAD
                     FLEFLLSLAFRRAGDCDALVPVDQAGRPEPLCAVYAKRCQPAIQEALLGGVRKITGAF
                     RNLRVYETPYADWAAYDAGGLLFANVNTRADLDAAHAAP"
     gene            24475..25236
                     /locus_tag="VFA54_09690"
     CDS             24475..25236
                     /locus_tag="VFA54_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS51121.1"
                     /translation="MPEDQQYPHYIEFRGVSKAFEQPVLIDCSFHLDSGKTLSIIGRS
                     GVGKSVTLSHLMGFLKPDRGRVIVAHEDITDYPESELNRIRKKVTMVFQSGALFDSLT
                     VAENVAFPLEDQGETARNIDEVVRGLLDMVEIGQYADMYPAELSTGMKRAVAIARALA
                     AQPEAILYDEPTTMVDPPASEHIGDLIQRLKSQLKLTSVVVTHDLDLMRKVADDVVFL
                     HEGHVIFFGPLDELESSQNPHIREFLTLDRFEVPD"
     gene            complement(25406..26890)
                     /gene="hemG"
                     /locus_tag="VFA54_09695"
     CDS             complement(25406..26890)
                     /gene="hemG"
                     /locus_tag="VFA54_09695"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HZS51122.1"
                     /translation="MRHVCIIGGGISGLAAAYYLSRRGCRCTLIEREARLGGVIRTEH
                     IEGCVVEGGPDSFLSQKPWALALIRELGIERDVIGSNDHLRQTYILRRGRLIPMPEGL
                     YLMIPTAMRPILTTRLLGWRTKLRMAADMLRRPAAGRAEEPGPGEDAESGAPFDSSTA
                     DVSAASFLRAHYGDEAVDYLAEPLLAGIYGGDAADLSARSVLPRLVELERRYGSLTRG
                     ALASRAQAAGSGSEPQAIFLSLRGGMQQLVDALARAIAGRVAVLRGEAERIEETGEGC
                     RIFFGGQWIEASDVIVAAPAYIAGRLLQAVEGCAAELLSGVRYHSSLTVSLGYNRAHF
                     SHPLNGFGFLTPKVERRRLTACTWVGTKFPNRVTGRRVLLRGFIGAGNEGDLLQCNDE
                     ELVEGVREELAQYMGVAAAPEFFKVYRWPRAMAQYNVGHERRIAEIQRRLAPFRWLHL
                     AGNAYSGIGIPDCIRTAKSAADRVLAAPAEAAPLAHTNIKSGQG"
     gene            complement(26887..27768)
                     /gene="hemH"
                     /locus_tag="VFA54_09700"
     CDS             complement(26887..27768)
                     /gene="hemH"
                     /locus_tag="VFA54_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZS51123.1"
                     /translation="MKQAVLLLAHGAPVSLDQIDEYLLNVRAGRPLAPAVVEEIRQRY
                     AAIGGGSPLLAITLRQAKALEAELGVPVYVGMRNWKPYIRDTVAQMAADGIGRAVAMC
                     MAPQYSTMSVGFYFRRTREALEQIGGRIEVSWVESFHRHPLLIAAFAERLRPALHAHS
                     CPVLFTAHSLPAKVLAQGDPYDAEVRATAAAVASELRLQHWDFAYQSQGLTEEKWLGP
                     TVESRIDTLCAAGAKEFVLHPIGFVCDHVEILYDVDIVFRQYASERGMTLRRPESLND
                     SPLFIQALAAVAREHLR"
     gene            complement(27796..28821)
                     /gene="hemE"
                     /locus_tag="VFA54_09705"
     CDS             complement(27796..28821)
                     /gene="hemE"
                     /locus_tag="VFA54_09705"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZS51124.1"
                     /translation="MPSLFEAACRREPVERTPVWFMRQAGRYMEQYRAVRSRHTILEM
                     CKRPAIAAEVTLQPVAALGVDAAIIFADLLLPAEPMGLKLEFVAGEGPHIANPVRTAA
                     DVAALESNHIGDLGYVAEAIQLVRRSLDGKIPVIGFVGAPFTLGSYLIEGGASRHYLH
                     TKRMMFGTPELWSLLMEKLVSVLASYARLQATAGAAAIQVFDSWAGALAPGDYYRYVL
                     PHSRALIRSVRSAGVPVIHFATGVGSYYPMLHKAGADVLGLDWRVELDQIWRSIRYRA
                     GVQGNLDPAALFAPLKELRIRVEDILSRAGRRPGHIFNLGHGILPETPVENVKAVVEM
                     VHEYGAS"
     gene            complement(28932..29378)
                     /locus_tag="VFA54_09710"
     CDS             complement(28932..29378)
                     /locus_tag="VFA54_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51125.1"
                     /translation="MNVSVQRLGFLAACGLLAGAAAFAGSLNGSAGDMPAYYDAQLFT
                     INFKQLPSGGENANLQHNGSINTIYMCDQCELGASGFLFIAVIDAIQGDGFNPLWAEV
                     QITFNSSNPPRQFTSDGQIVEAAQSGEITLTPTGELYRCSVVGAKK"
     gene            29724..30647
                     /locus_tag="VFA54_09715"
     CDS             29724..30647
                     /locus_tag="VFA54_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51126.1"
                     /translation="MPAPGALNYIEGQVYVDGRPLPPGAVRSTVLAPHQVLQTGQGKA
                     ELLLTPGVFLRVGDGSEMRMSSAGLADVKVAVIRGSAMLEAAELFKENNLAVSVGEAT
                     AKVEKKGLYEFSASQPAIAVLDGKAVVYQDDSHVTVQKNEEVLLEQHPLKARKLDEKT
                     IQADPLYRWSKLRSEYLARANIDTARTVVMYGGWYGPGWYWDPFWGFYSYVPAWGVIY
                     SPFGFAFYAPAWVWNAPIVYYHYPVIAGSYYRRRVPGRGFRSVPRIAHAFHGVPPGGF
                     RAATPRIPPRGIALASGSFRPGGVSGGAGRR"
     gene            30825..32054
                     /locus_tag="VFA54_09720"
     CDS             30825..32054
                     /locus_tag="VFA54_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZS51127.1"
                     /translation="MFPARSAVASCCFLSFSVLAGYAQGPPVAAPDAGSYVGSAACKT
                     CHASIYARWVKTRMANVVRDPREHPDAIIPDLSRPDPLVKFSKDDIAFVYGSKWKQRY
                     FKKVGDDYYAFPAQWDVTHRIWRPYVVQPGTDWWVRFYPGDNFQRPTGPLCDGCHSVN
                     YNIATHRVSEWNVGCEKCHGPGGEHVKRPLRSNIVNPARLDYVQANDVCIQCHSQGQP
                     LNNPIQGKYYDWPVGFRVGLRLSDYWKLEEHKLGETSFTHFADGTAHKNRMQGNDFVT
                     SLMYTRGVACFSCHDVHGTGNNADLVKPASVLCLECHGPNSPNGPHASSIEQHTHHKA
                     GSSGSECIACHMPQIQQTIADVNVRAHTFRFITPAKTETLKVPNACNACHKDKSTAWA
                     SEALKSWRDRSPWRMAE"
     gene            complement(32231..33535)
                     /locus_tag="VFA54_09725"
     CDS             complement(32231..33535)
                     /locus_tag="VFA54_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZS51128.1"
                     /translation="MKNRKSAWSRRRFLQGAGISPVAAGLAAGASARLAGQQTAQVQA
                     APDNIYAELGVRTLINARGVDPYYSSTLMPDEVHQAMRRASQYFVEIVELQKEVGQRL
                     ARFAGTEDAMVSSGAAACIAQATAGCMAGTDPEKIARLPDTTGMKNEVIITRRSVWDR
                     SIALTGAKLVVVSSLADLERSINERTAMMEFEYGDPGPVPLEQAISICKRHGVPFLLD
                     AAAMCPPFERLRFLASLGPDMFCVSGGKGLRGPDCTGILFGRSHLIEAALRNGSPYEG
                     SICRPMKVGKEEIIGVLAAVQWSSKRNYAADCRVWEIQMRQIVTALSKIPGVKAEIFY
                     RRLGNEVPHVGIRWDEKEFKLTRQQCIDALRRGKPQIEVIGEERGIVLPRVSAMLSEQ
                     GVQSGPAPLISIVSNTLKPGEEKIIAQRLTDILAPASQSNRA"
     gene            33601..35463
                     /locus_tag="VFA54_09730"
     CDS             33601..35463
                     /locus_tag="VFA54_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZS51129.1"
                     /translation="MRPFRVCSLILIVLSIFLLCLPAPSQRNAEYMWPLRPVVRGTRG
                     AVAGGTSMGTDAGMRMFHAGGNAVDAGVAALFAGAVVEYSHFGFGGEAPILIRTKDGH
                     VYSIPGVGTAPKLATLDYFLHRKLSPEEKMNPDADEREGPIPSFGILPALVPGMVDAG
                     LVALERFGTKSFGEIIQPAIELADGAPIDETRSSAIAHSVRYFQKWPTSQHVFMPNGR
                     VPRPGEIFRQPDLARTLRAMAAAEKSALARHKGDRVAGIEAVRDYFYRGEIARKIDAF
                     MRANDGLLRYEDMASFRIEPEEPVHTTYRGYEVYKSGFWSQGPAFLEALNMLEKFDLV
                     GMRYNSADYLHTMVEALKLAYADRDAYYGDPKFSPAPMELLTKEYAAQRDKLIDPHLA
                     SAEFRPGEISLRKPMHPSAYAGRINPLSDVLAAHDTTCLNAIDRDGVIFSSTPSGAWL
                     LPVSVIAGDTGIPLTQRGQSFLLIPGHPNVIEPGKRPRVTLSPTLVTRQGQPYMALST
                     PGGDNQEQSLLQIFLDVVEFGMNPQLAVEAPRFQTRHLVSSFDAHATHPNSLLLDERI
                     PTAVFRELAARGHRVERRPAWQNGAAPTAIRILPETGVIEAGADPYGMRYADAW"
     gene            complement(35544..36581)
                     /locus_tag="VFA54_09735"
     CDS             complement(35544..36581)
                     /locus_tag="VFA54_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZS51130.1"
                     /translation="MLAAELVGVRKLRVAEISNPPDPGPGEVQVRVRAVGICGSDLHC
                     YWEGGIGDTPCIFPMVLGHEPAGEVLRAGAGVTGLEPGMAAALEPAIYCGRCEYCLAG
                     RQNVCERIRFLSNPGEPGFFREVINLPAANVTPLPRTISMEEAVLHEPLAVALNAMRL
                     ADLRPGETAAVFGAGPIGLLTMAAARLCGAGRIYAVDRIPARLELARQVGADCAIDFS
                     AVPPDKAILAETSGRGVDVAFEAAGKAEAINHCLRVARNAGRVVLIGIPAELSVPVEF
                     HGMRRKELALFNVRRQNRNAAAAIWLLVERRLRAGEIVTHTRPLESIAAAFQLLENYD
                     DGVGKAVITMK"
     gene            36662..37489
                     /locus_tag="VFA54_09740"
     CDS             36662..37489
                     /locus_tag="VFA54_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HZS51131.1"
                     /translation="MSKRKVNESIKEFRVKPGAKVRLKNFDPARHWSPRHDPKLSKAL
                     ADDIGRMAELEVRLYAEHRRSLLIVLQGIDASGKDGTIKHVMSGLNPQSCRVHSFKKP
                     AGEEAEHDFLWRIHRALPGKGEIGIFNRSHYEDVLVPRVHQLVPKEVWEQRYQQINNF
                     EQEVAETGTEILKFFLHISKDEQRQRFQERLDDPSKHWKLSLPDFEERRLWGAYQEAF
                     EDMLSRCSTPWAPWHVVPANHKWFRNYVVAGTIVDKLEEMDPQFPPVTIDISKIVLR"
     gene            37575..38102
                     /locus_tag="VFA54_09745"
     CDS             37575..38102
                     /locus_tag="VFA54_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZS51132.1"
                     /translation="MAAPDVLPRLVLAKLREQIERTEHLIALIPPDKLGWKPDIPGAR
                     SIAELLGHLLDCLAGFCAALCAFDPVRLARFADLRGRKVNHACGTAEALDRVREYAGC
                     IEEGFALLAGQDLVRPVQTVFVPEGEPLLTLLLGNLEHLINHKHQLFFYLKLLGAPVS
                     TADLYRFRGPAPAAE"
     gene            38503..41481
                     /locus_tag="VFA54_09750"
     CDS             38503..41481
                     /locus_tag="VFA54_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS51133.1"
                     /translation="MRFQPKFLLPACIWLFIFPAAAQVDRAAINGTVTDASGGVIPGA
                     RVEVVALGTGLRREMLTGLNGGYDIPGLPIGSYNVTISKEGFRAVQFKDVSLFVGQTR
                     TLDAQLDVSAVASQVEVQATAAAINRSSAEIGGVIESQQVRDIPLNGRNWASLMTLAP
                     GAINVGGGDQRSIRFVGRSRDDNNYTFDGIDASGVQEQPQKSDVRLNISLESIAEFRV
                     NAAAYTAESGAAGGAEINVVSKTGTNQWHGGAFEFLRNDKLNARTPFDPSQLPPFHLN
                     QFGGSVGGPIARDRTFFFVNYEGLEQRLGQTLIGFVPSPSFRQQVIAASPALQSMMQA
                     YPAGQTPVDANTYQLTVQGRNSLREDSGMVRVDHRFTDKTSMFARYNVDDGLVQSPSG
                     ALGAKDTIAIRPSNLALQLMHIFSPTIINETKAGMNRSAYVHTVVGIFPQDISAPGFD
                     DLPNSSLDEEIGTTFTYADNLTIIRGRHTFKMGADIRRVRLNNSGNAIDTTTISYASL
                     KDFINNAVDSISVNAALGIGGMRRTFWMGYGQDEFKARPNLTINLGLRYEYYSVMHEV
                     KNRALVVDILGCGGFCPVGTPFYSPDRDNFAPRVGIAWSPSSLKGKTVIRTGFGIYFG
                     ANQNDDFSDPHESTAGRFALSSADVPNLSYPITPFLGQLQSQGLSPKGIDRNRQDPYY
                     ENWDFLIQQQLPRNFVGQVGYVGSEGHRLFTSRAVNLINPLTGKRPLSQFGQFNVKSD
                     DANSNFNALQASLQRSFTGGWLWQTQYMWSHAITDGSVGAGESVAVQNASCRACDRSD
                     SPYDVRHTITINSVYQLPFGPGKPFLHAESLAGKLIGGWELSGLGAASTGRPVNILVT
                     RSASSLLDGNRSNQRPDLVPGVPIYPASGRSIYQWFNPAAFAVPAKGTWGNLGRNIAR
                     GPGLWEIDTALQKRIPVFERANLSFRAEAFNLFNHPIFANPAANISSSSFGRITSVLN
                     NGAVGTGAPRRLQFMLRLDF"
     gene            41492..42925
                     /locus_tag="VFA54_09755"
     CDS             41492..42925
                     /locus_tag="VFA54_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZS51134.1"
                     /translation="MTTFRGAVLAALIGLSGILCSAPSPQGVDAAQSPFLVKPYLQLG
                     NYAQPSNPERLALLWHTAGADADWRVEVKSSAANEWSACDSPVERRIAVSGIPPHRVY
                     RAVLSRLPPGEEFEYRVLKNGKEVFRAQGRSRKPVGRPYRFVVFGDCGAGTASQAAVA
                     YQIFQAKPDFVVTAGDIVYGNGRISEYRTKFFPIYNADGPSMTGAPLIRSTLFLAAPG
                     NHDMSNRFPDGLAYFLYWDQPLNGPIAESSSMHAPALPGDEDQAAFLRAAGSTYPRMG
                     NFSFNYGNSHWTVLDSNRYVDWNSPALRAWVAGDLSAARRAAWRFVVFHHPPFHSSHR
                     HFEDQWMRVLADVFQAGNVDIVFAGHVHNYQRSLPLRFTPKAGSDGNLLARSGRVDGA
                     FALDRNFDGKARTRPNGILYLVTGAGGAGLYDPDQQNDRSSWQEFTARFFSNQHSFTL
                     VNVDGKRLDVTQVAEDGAVLDQFTVSK"
     gene            43191..43346
                     /locus_tag="VFA54_09760"
     CDS             43191..43346
                     /locus_tag="VFA54_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZS51135.1"
                     /translation="MRSIGFPELLIIFGIAVLFFGSNKIPDLAKGLGEGIRNFKAAIK
                     GDEPPKA"
     gene            43762..>44065
                     /locus_tag="VFA54_09765"
     CDS             43762..>44065
                     /locus_tag="VFA54_09765"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZS51136.1"
                     /translation="MSKSARTVLVLLLVMVLCGGGLLSAATRPIVMGTHGMAVSGHPL
                     AARAGMKMLEQGGNAIDAGVAQVFAQAVLEFNLFGIGGECPILIYSAKDKKVYAING"
BASE COUNT         8650 a        13837 c        13220 g         8358 t
ORIGIN      
        1 gtccgggaag atcaagggcc gcattcccga ccttccaatc gtgacgatcg ccagtgccca
       61 agcggcgctg cgcgtcagcg cgccggacgc ggcgcctgat tcctggcaac cggtggtgcg
      121 gcgcgaagac cgcgaacgtt ggaatgactg gggcatcggg ttgctgctgc aaggagatct
      181 caagggcgct gaatacgctt tccagaaagt caccgaggcg gagcccagct acgcggacgg
      241 ctggctgaat gtggctcgcg cgtatattca ggaagggcaa accgatgcgg ccaagcctta
      301 cattgaaaaa gctttgtcgc tcaacccgca tctcggccgc atttattatt tccgggcgct
      361 ggtggaaaaa gcggatggcg attatgatgc cgccctgcgc tcgctgcgcg ccgctgcggc
      421 ccaatatccc cgcgatcgcg tcgtgctcaa ccaaattgcc cgcatcctgt tcttaaagcg
      481 ggattatcgg ggggctctcg ccatactcga ccgtgtatgc gccgtggatc cggaggacct
      541 ggccatgcat tacaccaaga tgctgtgcca tcgcggtctt ggcgaggtcg atgccgcagc
      601 ccgcgaggaa agattgttcc ggcggtttaa agcggacgaa gcctcgcaag cgatcaccga
      661 gcgcccgcgc cgcaccagtc cggaggacaa caatgagcgg cagccgattc acgagcacga
      721 atccgctccg atcgcaaaag cggtccggcg catcgagcct gcgcccgccg tggcggccgg
      781 aggcggcagt tgaagccgcc gcgaatggga ctgggcgcgt ttctggccgc ctgctgcgtg
      841 gtcattgccg cggcgccggc gggagttcaa tttaccgatg taaccacgac tgccggcatc
      901 cgcttccgcc acaatacggg ccgttcgggc aaaaaatatc ttcccgaaac tctcggctcc
      961 ggatgcgcct tcctggattt caacaatgac aactatgcgg atattctcct cctgaacggc
     1021 aaggattgga atccggcggc aagcgcggcg cggcattcgt atccggctct ttatcgcaac
     1081 aatggaaacg gttctttcac cgacgtgacc gccgcagcgg gcctggcgat agagatgcac
     1141 gccatgggcg ttgcagtcgg cgattatgac aacgacggct ggcaggatct ctacatcacc
     1201 gctctcgacg gcgaccgctt gcttcacaac gaacacaatg gaacattccg cgatgtgacg
     1261 cgcgccgccg gaatcaacaa tgccggcttt ggcacaagcg ccgcctggtt tgattatgat
     1321 cgggacggcc agctcgatgt gttcgtggcc aactacgtcc agtggtcgcc ccagaccgat
     1381 ctctggtgtt ctctcgacgg gcgcaccaaa tcatactgca cgcccgaatc gtacaagggc
     1441 ctgcccgcga agctctatca caatctcggc ggcggaaaat ttgaggatgt gtcccgcaaa
     1501 gcgggtatcg ccgacccggc tggcaaatcg ctgggagtgg cggtcctcga ttttgacggc
     1561 gacggctggc cggacctgtt cgtcgccaac gacacccagc caaacaagct ctatcgcaac
     1621 aaccgcgacg ggacctttac cgatataggc gtggctgccg gagtcgcctt cggagaagat
     1681 ggcgtagccc ggggcgcgat gggagtggac gccgccgact atgatcgctc cggccgcccg
     1741 catcttctgg tcggcaattt ctccaaccag atgttggccc tgtatcacaa cgaagggaag
     1801 gctctctttg tggacgaggc tcctgcgagc gccgtcggcc gcgcgagcct gctctcgctt
     1861 acttttggtc ttttcttttt tgattacgac ctcgacggcc accctgacat cttcaccgcc
     1921 aacgggcaca ttgaggacga aatcaaccgc gtgcagccca aagtccacta cgaacagccg
     1981 ccgctgctgt ttcacaattt gggagggaga agatttgagc tggctaccga ccaggtcggg
     2041 ccgtttcttc gccggccgat cgtcgctcga ggggctgctt acgccgatta cgatctcgac
     2101 ggcgacctgg atatcctgat caccaccaac ggcggcccgg catatctgta tcgcaatgac
     2161 ggtggaaacc ggaacaattt tctccgcatc aagacgaggg gcagcaggtc gaaccgcgat
     2221 ggcatcggcg cggtcgtccg cctgcggtca gcttcgggat cgcaatggca aagcgtccac
     2281 agcgggtcga gctattgctc tcaaagcgaa ttgccgctga ccttcggttt gggccgcgac
     2341 gccagagtag atttgctgga gatcgactgg cccagcggag cgcatcaggt tctttccaac
     2401 ctccccgtca atcaattcat ccttattcag gaagaccagc agtctgtttc cagaatccgc
     2461 tagggcgggc tggataaaga ctgtgcccta tcctggcggt ctccgtacag aagcgggact
     2521 gcatcgtgaa gaacgatccg gcaggctccg ccttgccggt tgagacagaa ttcttcaagg
     2581 tggtaccatt tttatattca tagattctga gcgcaggtat gcggtgttgg cgcctagact
     2641 gccgctcggt caaagagttc cccaaaatgt tcgctgaaac ttcgttcagg aggattctat
     2701 ggctacagcg ttccagcaac atgatccagc cggcggtatt ccgcctttcc ggctggatgt
     2761 gactctgcac gatctgcttc gaaaacaggc ccccagacca ccggcgccgc ttcctgcggc
     2821 cgaaccgcaa aagctgcccg acccggtgtt cccccagcat ccggaactgt tgggcgacca
     2881 tggagactat gttcccgcgc acaggccggg tgcttctcct taccgccgcc ggtggacggc
     2941 gccgctcgtt tatcgggcga tgcgcggctg gctgtttcct tatttcaaat ctcggattct
     3001 gccgggagat tttcacccca tcatcgctta tctcttcacc gaatggaaat gcaatctcga
     3061 ctgctactac tgctgggcgt ttgacaatca cgtaaagggg atgagcgagg ataccgccaa
     3121 gaggtcgatc gattggctgc acacgaccac ttgccgggtg ctggcgctta tgggcggcga
     3181 gcccctgctg cggcctcaat ttgtgcacaa ggtcgtgtac tacgcggcca aaaaagggtt
     3241 ctgggtatat ttgcccacga acgcgcggct gctgcgcccg gatgtgatcg accgcatagc
     3301 cgacgccggc gtggcgatcg tgaacttcgc ggtggacgta gtggacgaaa agccgggcct
     3361 gccgaaagcc ctgaatcctg tccgtaagta tttcgactac ctgattcgca aacagtataa
     3421 gtacggctac tgcgtgttct tcaatatcaa catttgccgc aataatctgg aagacgtcaa
     3481 acagctcacc gagattgctc acgataacgg tattgccacc gactaccaca tcaatgaatc
     3541 gcccatgatc gagcaggacc acttccagca cctcgaagaa aatgacacct atatccggcc
     3601 tgaggattgg cccaaagtgg acgggcttat cgactggatt atcgagaaga accgcgccgg
     3661 ctataagatg gtcaactctg tgaggaggct gcaagacatg cgcgatttta tgcgcggcaa
     3721 agtcgagccg tggaactgcc gcgccggcca gaactcgctg attatccggg tggacggcac
     3781 gctggccccc tgcttcccga tgtattccgc tacgtatgat tggggcgcta tcgagaaccc
     3841 gaaattcgag gtgaagcagc tcaatgaaat gaagcagtcc tgccagaccc actgtttttc
     3901 tacgctgaat cataacctgg ggtactgcta caacgacggt cgcgtgatca agtgggttct
     3961 acggcaggcc gcccggggct tccagggagt ttcaggaagc ttttacgatt gaaccgatcc
     4021 ggccggtctg ccctggtaca acaggccccg gccttcaacc ggggcgccaa gttcaaaaaa
     4081 gagcatcccc gttcgagaac gggagtacta aggtggaagc gtacgtggaa tttaggtgat
     4141 cgaagtgtag ctttgccggc tttaccgcgg taccttggtt ggcggaccgg catgcgcatt
     4201 gtttctaccc ttctggcggc tgctctcttc acgattgcgg gattctcgca ggaaaatagc
     4261 ggaggcctcg atcctgcggt acggggcttg ttacggcccg agagcggctt tctgttgggc
     4321 gtccaatggc gcaaagcgct cagcctgctg ccggcggaca ttttgaaaaa gcaactcgcg
     4381 gtctcgggtc ttgcggcgtc ggcggacgcg gcggacgccg aatccgtaat cctggaagac
     4441 atcgatgccg tgtacgtcgc cggctccaca gcccgcgacg ttgcagcggg ccgccagacg
     4501 ccggttctgg cgatcgtcag gggccgtttt gaccgcacgc ggatgcaggg gctgctgcga
     4561 ggccacggcg agatctaccg gcgggttttg ttaatgacac cgcccggcgt cagcccgccg
     4621 caaacccgcg tcgccatgct cgatgagggc accattctga tcggcgaccg caaggagatg
     4681 atggcggcct tggacaggct ggcggctccg tccgcgcccc agccggcggg aaaaaccagg
     4741 accacgctgc tcgaccgcgc ggaagtcttg gccaaacaaa acgacctgtg gctcctgatc
     4801 gacgctcctc ccggcggctt tcaaccccct ccaccgcaag cgcgcggttc cgggacttcc
     4861 gccgcctttg tcccctcgcc ggagattctc tcgaatttga gaggcctgga tctcggcgtt
     4921 tccttctccg gcggattgcg tttacaagtc ggtctgcgcg ccaaatcggc cgaggcaggg
     4981 aaaaacctgg cagcggccgt tcagggtttg ctggcactgg ccgccctcag ccagcaggca
     5041 aagccagaag cgctggatgc gttgcgccgg atcgagatca agccgcaggc atccactgtg
     5101 aatgtcagcc tctcgctcac cgagcaggaa gtcaggaaag tctacgccga gatgcaagcc
     5161 tctgcgaaga acgcctcggc tcgccgcttc ggcggccctg ccgtctcagg cggaaacgtc
     5221 gcggtctcgg gagcggaccg gccgagcccg gcggggaaga cgccgcgccg cggcacaatc
     5281 cgtatcctcg gtcaagacac cgggacggtg gaagtgccgg ttggcggcca aaagtaggat
     5341 cggatttccc cggtccggag ctctcgggag cgcttctcca tgcttgagcg ccggccgcag
     5401 agcgaagcag ggttgcttca aactttcccg tccagtacgc aagcggtacg ccggatcgaa
     5461 cgcgcccaga cattgcgctc ggaagacttc aagcgaagcg ctagcgttac cgggccaccc
     5521 agagcccgat ccggtcgtaa gcatactccc gcacttgcat ctcgagcgag gcgtaacgga
     5581 gcatccaggc ccgctcccgc aggatcctcg ccggttccaa aggcagttcc ccgcggtaca
     5641 catcggctcg atcgccataa gagtactgct gatactttaa tggaaggcgg gacgcggttt
     5701 ccatgagggt ttcccagaaa ctttgccgcc ggaaccaccg cttccgggaa gcggcctcgc
     5761 gcggcagttc aatcacggcg cgcccgcctt caagagcggc gcgcacgcat ttcgatgcat
     5821 cgaaaggacg cccgggctct gggtcgggaa acgcggcgaa cgctccggca atctcctccg
     5881 aagaagcttc gatcggactc cagcggatcc gcgaagcgcc gctgacgacg attccttgcc
     5941 ggagaatctc ctgggctcgc gccacatcct tgccggccac ccgcaggatg tgctcgatca
     6001 agtcgggcgc ccgcatctgc tcggccacca caggcgtgat gctgacgtag ccggcagcct
     6061 ctgaactgat tttaacgggg atcttttccg gaaacggcaa ccactacgcc ttcttcacct
     6121 tcccgctacg gatgcaggaa acacaaacgc gaatggtctt gtgagcgccg ccgacgacgg
     6181 cgtgtacgcg ctgcaaattc ggcagccagc gccgcttggt cagattgtgg gcgtggctga
     6241 tccggtgccc ggtcatgggt ttcttgccac aaagttcaca cgcttgcgcc atagctctgc
     6301 tttccgacct cgaatttatg agtctaacat ggttacgttg gcgctgccaa ccggacggcc
     6361 gagagcgtcc ctccgcgggg aaccctgcgc cctgccgttc cgtagtaatc cgtatgcgct
     6421 ggcgggaact gtttgccgag accttgcgcg ccctagcagc caacaaaatg cgcagttttc
     6481 tgaccatgtt tggcatcgcc tggggcatct tgtccatcac gctgatgatt gcggcaggcg
     6541 aaggcctgaa aatgggtcag gctgcggccc agcgcactct cggcaaagat atccttatcg
     6601 cttttcccgg ccgcacgtcg atgcaggccg gaggcctgcg ggccgggcgc cgcattttct
     6661 tccagctctc cgacctggat gcagtccgcc gcgattgtcc ggatgtcgcc cagttgacgc
     6721 cggagcttgg ccgtccgggc gtttacgtcc ggtcggctta taacagcgga agctttcggg
     6781 ttaccggagc cctgcctttt tttcaggata tccggtcgct tgacgtcgcc aaagggcgct
     6841 tctggaccta cgccgacgag cagatgcgcc tgcgcgtggc ggtgctgggc gacgaggtac
     6901 gcaaacagtt attcgcgtcc cgcaacgctg tcggcgaaac gatccaggtg ggcgattttc
     6961 cctatcaggt gatcggggtt ctcgcgcgga aagaccagga ttccaactac gacggtcccg
     7021 ataatcagaa gatcttcgtc ccgctggccg ccgtcagcac cgacttcccc agtctgcccc
     7081 cggccgcccc caatagcgta gatcgcgtgg tcgccacgcc ggtgtccgtc agccgccacg
     7141 aagaggccga agccgagctc cgccgcgcct tcgcgcgcct acatcaattt gatcctgctg
     7201 ataaagacgc tgtttttgtc tggaacacga ttaagagcgc gaggattttc agccgggttt
     7261 tcgactctat ggaactgttc atgggcgtgg ttggcgtcat cacgcttctt ctcggcggca
     7321 tcggcgttat gaatatcatg ctggtgtcga tcaccgaacg cacgcgcgaa atcggcttga
     7381 ggaaggcgct cggggcgaca cgacgcgtgg tcctaatcca gtttttcagc gaagcgctgt
     7441 tgctcactct ggtaagcggc ggcgccggtc tggcgatcgg atacgggatc tgcctcgccg
     7501 tgaaccggct tcccatgccg caatatttcg cgggcttgat ggtcacgccg tgggtgggtt
     7561 tgttcgcttc cctggtgctg acccttgtcg gcgtcgcgag cgggctttat ccggcgaaag
     7621 tcgccgccga tatgaccccc attgaggcgc tgcgatatga gcggtgaaga aagccgctcc
     7681 cattttatac gccgttttct ggaaggtggg caggtctgag acgttatgga actgattcgg
     7741 caagccgcga tggctcttcg gcgcaacatc ttccggtcgt gtctcacggt actgggaatt
     7801 gtctggggca tcacggcggt gagcatgttg cagtcctatg gcgatgggtt ccgccaggcc
     7861 gtagttcgcg ccttcgacgc cttcgggccc gacgtgatta tggtctggcc cggccagaca
     7921 agtcagcagg ccggaggcga gcgcgcgggg agaaagatcc ggctggagct cgaagatgta
     7981 cgccgcatcc gcgaagacgg cacgctgatt cgcagcgcca gcccggagct ttttaagccg
     8041 gcgcaggcgg tcaacggagt gcggcaggcc agcgtcggcg tgcgcgccgt gcatccctgc
     8101 tatggggaaa tccgcaacga aacgccggcc gagggccggt ttatcagcga cgaggatctg
     8161 gcccagcgcc ggcgcgtggt gtttctcggc agcgaggcgc ggcgcaagct gttcggaaat
     8221 atcgacccga tagggcaggc gattctgctg aacaacgccc gttttatcgt catcggctgg
     8281 atggaaaaga aactcagctt ctccagctac ttccgttcgg atgacgagtc cgttttcatc
     8341 cctttgaccg ccggcgccga tctttgggat acgcgttatc tgagcgttat tgtcgcggag
     8401 ccggtcgccc gcagcttcag cgccgccgcc gttcgccagc tccgcgaaat tttggcgcgc
     8461 cgccagcggt tttcgccgcg cgacgagcgc gccgtgcaga ttttcagccg cgaacaagtc
     8521 cgcccggtca tcgatgggat taccattggc atcgagcttt tgctcatcgt agtgggcgcc
     8581 ctgacgctgg gcatcggagg cgtcggggtg atgaatatca tgctcgtatc agtccaagag
     8641 cggacccgcg agatcggcgt ccgcatggcg atcggcgcca cacgccggca aatccgaatc
     8701 cagtttctcc tcgaagccct ggtaatcgta gcagccgggg gcctcatcgg tctgggagtt
     8761 tcctatgtcg tggtccactt tgttggggtg ctgccgttgt tgggccagac ctggaatgat
     8821 gactcgggcc gcgccgacat ccacttgcaa atgtcctgga tcaccacgct gcgttcgatt
     8881 ggtttgctgg ccgttgtggg cctgctcagc gggattttcc ctgccatcaa ggcatcacgg
     8941 ctgaatccga ttgaggcgct cagatacgaa taacccgcag ccctccgttc ggccgtaagc
     9001 ggcgcaaggg cggcagcggt gatgccgatt gttcgggacg gggcgcgccg gccgggcgtt
     9061 tgctgccgcc ggttacttga taaatccggc ctttctcagt tgttttccga gatccggctc
     9121 gtcgaaaaac gtaactttat cgaccaggcg cccattttcg agagcgacag cgtacggtgt
     9181 gtcctggaat gggaaggact gtttcagttt ttcgagatct ggtgaaagca gcgcgctggt
     9241 aagcccggag gattgtagaa atcccggcgc atcattcatc gcctgcgtgg ggactgcgat
     9301 gatcgccgcc tgccagctca agaccgacat cgcctgagcg gcctggaagc aatgcggaca
     9361 atacggattg aagaagaaaa ggaagacccg gccttcccgc aaagagaact gcttgccgcc
     9421 ggcggtgatt gactccgggc ccgtcacgcc ctggcctcgt acgcggtcca tcgccacgga
     9481 agctccggcc agcaacagaa ccagccccag catcgcgctg gctttgccac cgccgcgcga
     9541 cctgccggcc atggcgccgg ccagcactgc caccgccatc atggcgccgt cctcgacgaa
     9601 aaacatcggg ccgatggccc gcttgagcca cgggaagcaa ctgcaatcgg cgccggtgag
     9661 agcgcggtag tggacgccga tgtagatcat aaaggcagaa agcagaaaaa ccgaaagcca
     9721 cgccccccag cgcctccatt ggggggccag cagcaggatg cccgcgaacg tttccgcagc
     9781 gccaaagaac acggcgcccg ccatgctgat gccgtgcggc accagcatct gcgtcagttt
     9841 tacctgccag ccgctcaggt cgctcaactt ccacaggcca gcgctgagcc acagcgcggc
     9901 cattgccagg gctgccgccc attcgatggc ggttttccat ctcggccgga agaaagaagc
     9961 cgatagagcc ggtcggtaat ttagggaatc cgattcagtc atggtggcaa agccggatca
    10021 gcccaggacc gctttcgggc ctacaatcta gagtatatgg taagacgctc ctgcgcgggc
    10081 tgggtaagct ttctggcctg gctaacgtct ttcgcaactg ccggcgcggc cgcccagcat
    10141 cgcgaaacct tcaattacag cttcgacgta gattcgggtg aatctcgcta cttcgagatt
    10201 cccaccaagg aagccggggc gcggctccgt gtggagtttg aagtgcggtc gcctcaaaga
    10261 ttccccggca tccgggtggc agtggaaaag caagaccaat tcgagcgcct tcgccagcat
    10321 cagccccatc aggatctcgc cttcctgagc taccgccgcg aggggaccct gcaagtgcaa
    10381 ttgcccgagt caggccgcta tgccgtggtc atcaacagcc ctccggagac tcaccgccgc
    10441 tgccgcgtgg aaatggacgt gacgcttacg accgggcccg atcccgaaac gctgcccgtc
    10501 agctatgctt cgccgcgaac ccggctgatt gtggtgaccg ccagcttggg cgggtttgtc
    10561 ttgatccttc tgctttcggg tcgggctctt tggcgtgctg ctcgccgcag cctccgtctg
    10621 ttttgagcag gcttcgggaa gcccgggggg acgcccggtt ccggccgcat gggaatggcc
    10681 cggattctct ggctgcggcg ggcgactatt tccgctgcct ctcctcgatc atggacttca
    10741 gagattccag ctcatcgcgc agcttgcttt cgcggcggcc caggctgaac aagtacgcga
    10801 ccactagaac ccaggccgca gagaacgcat accaaagcca tttgaagttt tcttccatat
    10861 aaatctttct ttacgcctcc gagtgcacgg tgcgccgcag gccgtcgatt tcccgccgca
    10921 accgctccag ccgccgccgg atttgcagca aagctccgaa gagtaccagc atggtggcca
    10981 gggagaacag aaacacgtac cacattcttg ggtcaaggcc cgagccctgg ccgcccgcca
    11041 ttaccggctg gggatgctgg gtgcgccacc agcggttagc catataaaca atcacgccgc
    11101 cgaccgcctg gaagataacc acaacggagc acatcaccgc gcgcgcgctt gggtcggcca
    11161 ccgcccgccg caagatcagg tacccggcaa acattagcca caggacgaac gcggaggtca
    11221 gccgcgcgtc ccaggtccac cagatgcccc ataccggccg agcccaaaaa ggccccatga
    11281 ccagattgat gctgtcgaaa acccagccta cctcgatggc ggagacggcc aggtcgtcga
    11341 accggtaatc gcgggttatc aggaaacgga tactggccac gaggccggcc agaatcgcca
    11401 ggctggcgac gaaaaacggc ggtacgtgaa aatataagat ccgctgcacg acgcccatgg
    11461 tcctctccat gggaacccac agaaatgtca gatacagcgt gatgaccagc atcacaccgc
    11521 tcaccgcggc cagaatcgcg ctggaccgat tcttcatatc ttcatcccac cagtacgctt
    11581 tccacgagcg ccccggccag tacggtgaaa ataatgtcgc agccgatgag cacgcgcagc
    11641 caaatcatat cgggcgcaat gggctcgccg gaaaacaggg tcgtcgtcaa ttgcaccgct
    11701 gccatcagaa tgggcagtgt aatcggaaac acgagcagcg gcagcatcgc ctcgcgaagg
    11761 gagacgttca ctgtcaacgc gctgaaaagg gtgcccacga cggtcaaacc ccaggtgccg
    11821 agcagcagca ccagcagcaa ccagcccagt cccacggtcc accgcacatt gtaaaagatg
    11881 ccgaagatgg gcagcgtgac cagctccagc accaggagca gaaccgtgtt cgacaccgct
    11941 ttgcccagca ggataggcgc gccggaaagg ggagaagcca ccagcccatc caggcaatca
    12001 tttacggttt cccgggcgaa gctgcggttc agcactagaa ttcctgcaaa gatgaatacg
    12061 agccacaaaa gcccgccggc aatctcccgg gtctcctcgg cggagggatc gaacgcgaag
    12121 ctgaacagca ggaggatcac cagtgcgaac gacagggctg cgttgatgac ttccttgctg
    12181 cgcagttcga cgcgcaggtc cttgagcgcg atgataaggg ttccgcgcag cacatccatc
    12241 aggcccgcgg ctccgtatat gtgcggtaaa aatcttcggg cgcggcacgg agctcagcgg
    12301 tgctggggcc tcggaaggca agccgtccgc gatcgagaat ggccacgtcc gtcgccagcg
    12361 ccagggcctc gcgcaaatta tgggtgctga tgacggcggt gcgtcccgag gcgtgggctt
    12421 gctcgatcag gcgcgataac aacgcggcgg cccggtcgtc gagagccgtg aaaggttcgt
    12481 cgagcagaag aatttccgga tcgtgaaaaa aggctcgcgc aatcgccagc cgctgccgca
    12541 tgccgcgcga gaactcgcgc gcctgccggt tgcgcacgtg atcgaggccc gtcgtctcca
    12601 gccagtggtc gattttgtcg tgcggggcgt gagggccgta aagacgggcg aaaaactcaa
    12661 ggttctcggc agccgtcaag tcgtcgtaga tccaaatact gtggccgatg acgccgatcc
    12721 ggcgccgttg cgcggggctg cgtagagtct cctcggctcc agcggccgcg ccggaaatgg
    12781 taatacttcc gcgcgagggg ctggagagcg cggcgaacat ccgcagaaga gtcgtcttgc
    12841 cggcgccgtt gcggccgagc agcgccagcg tgactcccgg ggccacatca agattgatgt
    12901 cgcgcagcgc ggcgaaatcg ccgtaaaatt tccagactcc cttagccgag atcagcgggg
    12961 cggccctcat gccttgcgct tgcttcgcgc gcgggaactc ttctgctgcg aagggccggc
    13021 gtttttcatt gacattgcct gcggcttgcc cgcactctgc gccgcggcct ctgccgcgcc
    13081 gcccggcccg gcgttgtaaa gagcataaaa gccgagcgcc agaatcccca tcgcaaagcc
    13141 gagcaccagc cagagcttgt tgttgatcgg cgccggagag atggaaatcg aatcagcgtt
    13201 ctgctcgccc ccctgctcct cgccccgcga cagtctccct tcccccgaga tcgtcatctt
    13261 ataagacttc gcttccggga ggccgaaaat ggacgctttg atctgcggat tttcggggag
    13321 agcctccatg ccttccccct ggagagtcac gccgctgggc gcagctaccc gcgtcgtcag
    13381 ccagtcataa gcgctgcgaa cctcgaattc caatggggag tgatacggca cgagataggt
    13441 gatatccaga cgagtctcgc ccggcttgat cggaaaattg accttgtaga aatcgggttg
    13501 attggctttc tcggccgtct gcttcagcgg catgccgcca ggacccgtgg cgttcacttg
    13561 cactacgccc ttcgccgccg gcggcagata gaagcgcagc gtgccctgtt tggggtcaac
    13621 gtaagtaacc ggcggctgcg cgttgttccg gtaaacgtaa aattcgctga caatcatctg
    13681 tccgcccgag ggttcgagca gaatgatcct ctgttcggga gccccggccg tgtcgcgagc
    13741 cggcgcggac cggtaaacgg tcagccgcac atcggcggcg cgggatccgg gaggaatcat
    13801 ctgattgtag ttgacgcctt caaagtcgcc gcgcagcagc atcggagcgc caggctcggc
    13861 gtcaaccgca aaccggaatt tccctgcggc gtcgctcttg gttgtcccca ccgggaccat
    13921 gccctgatcg agcttgatca aagtcacttc cacgcccggt tgctcgcggc ctgtggtgcc
    13981 gttgatcacg acaccttcga ccgcggcggc ggccggggtc gccagcagaa gagcgcacac
    14041 caagccccgg cgaaacagcg gcagaacagc ggcccctggc cgccttcttt gagcccccat
    14101 atcctcgtcc tggcgcagcg gcaggagttt tctcctgatc tggatgttga aactggctgc
    14161 tttcatgacg tagctgcaat ggcctgcccg cattcgccgc agaacttcat gggctgcgga
    14221 aactcagcga gacagtgcgg gcagacgcac tttgccgggc tcttcatgtg cgctcgcgcc
    14281 gctcccgctt cctgcgggcg cacggtcccg gctccctccg ccggcttctg cgccgttttc
    14341 ttcaagacat tgtcgatttc cgccagcaca gcagccagct cgcgctgcaa ctcgagcttg
    14401 gtcgccgcgt aatcctcgtc ggagagcttg cccatacggt attcaaactg caaatcccgc
    14461 agattgtcgt agatcgcctt cttcttctct tccaagtgca gggtgggcga aaccggttcg
    14521 ggctggggca agtccttttg gcggaccacc agagtgaacg ccaggaccgc aacgatcaaa
    14581 acggcagcgg cggcaaaaat catcaaatgt ctatttgctc cacctggctg gcgctaggca
    14641 ggtcgcatcc tccgccgtgt cactcggctt cttccagctc tttttcgatc tcgtcacggt
    14701 acttttccag cagcaggctg gagggcgcgc cggcgcccgt ctggctcccg gcagcgacgg
    14761 tggccgccgg ttcaggccgc atccatcgct tcagcaccat gcggatcacg atcaaccccg
    14821 ccgccagagc tgcaaaaggc atgatccagc tcagcaggct gaagccttgg gctggcggcg
    14881 aagccaagat caaggtccca aacttctctc gaagcttcgc caaaatggcg gcctcgtcca
    14941 tcccggagtc cagcatcggg cggatttcgc tgtttacctg gtcgcgatag cggcagcccc
    15001 ccatatcgca atcgccgacg gttttgccgc acggctcgca ttggcattgc agcttttgcc
    15061 cgatggcccg gagccgcttg tgcatcgggt cgcccgtgct cacggtttgc gccgaaagcc
    15121 cgacaccgag taggacgatg gcacagacgc agccggctat ccacgcggcc gcgttggggg
    15181 ttttgatcct gccgcccggc aatccccagg ccgccgcttc gttatctttc cgccaaaacc
    15241 tcatgtgttt ttgccgtagc gattacctgc gtgcgagcgt aggacagctt gactttgcta
    15301 ggcactagag cggtcagagt tcccagtatc acgacgaacg cgcccaacca cacccagttc
    15361 accaatggat tgacaaatac ttgaaagacc ggcttagagc catcattggt cctgccggcg
    15421 aacgaaatgt agagatcctc gctcagccgc ggccggatgg ccacctctga agtcaactgc
    15481 tggctcgcgc gatacagcct gcgttcggga tacattttac cgagcggctg cccgtttttc
    15541 gaaacctcga tcgtggctgt ttcgtacgcg taattcccgt tctcgccctg cttgatatcg
    15601 gcgatgcgga acgcataggg ggcgacttgc agggtgtcgc cgacttgcaa tgcatcctgg
    15661 gcctcgcggc tgaacacctg gccggtgaag ccgatgaaca tgatcacaat gcccaaatgt
    15721 acgatgtagc cgccgtagcg gcgggtatta cggtgggtca actcgaccgc gcagccaaga
    15781 agcgacccgc cgtttttcgc ctggatagcc cgcgcgcccc tataaaactc ggccagaacc
    15841 gttacgccga caaacgtgca cagcatgaaa gacatcaggg catagaaatt gcgagtcacc
    15901 gccgccagca gcagcgcgcc cagcgccagc gccgccactg agggccaaag gaaattccgc
    15961 ttgaggctct caaccgatgt gcgccgccag gcaaacagcg gccctacgcc ggtcaggaac
    16021 agcagaaaca gcgcgatggg cacgttcacg cggttgaaaa agggcggccc cacgctgatc
    16081 ttcgagccgg tcacccattc gctgatcacc gggaacagcg ttccccacag cacggcgaag
    16141 caggacccga ggaggaccag gttgttaaac agaaagctcg actcgcgcga gaccaccgat
    16201 tcgagctgat tttcgctctt caagtaatcc agccggttca gaatcaggta aatggttccc
    16261 gcgatgccga tcgccaggaa agtaacgaaa tatttcccga tgggaccttc cgcgaaggcg
    16321 tgtacggaac tgaccaggcc gctgcgagtc agcatcgtgc cgaaaatgca caggaagaag
    16381 gttgcggaga ccagcaccat gttccacacc ttcatcatgc ccttcttctc ttgcatcatc
    16441 accgagtgca aaaaggcagt cgccgtgatc cagggcagca gggacgcatt ctccacaggg
    16501 tcccattccc aatagccgcc ccagccgagc acggcgtacg cccagcgcat cccgaggcag
    16561 atgccgcagg tctgaaacag ccatgtcacc atcgtccaga tccgcgtggt tccgatccat
    16621 ccgtcgccct tctggcgggt gatgagcgat gccatggcga aagcaaacgg aacggaaaaa
    16681 ccaacgtagc ccagatacag catgggcgga tggatcacca tggtccaata ttgcagcagg
    16741 gggttcagtc cctggccgtc cggcacggcc atgatttgcc cggccgatgt cagaactctg
    16801 aagggattgg cgacgaaatt gaccaaaacc aagaaaaatg tttcaatgcc gccgagtatc
    16861 gcgatgacat aggcgatcat atcccgatgc ttgcggcgat tccagtagac ggaaaccaca
    16921 caatacgtgc tgaggatcca gagccagaac agcagtgatc cttcctggcc gccccaccag
    16981 gccgcaaact tatatatggc cggcaaatcg cggttgctgt gcgcggccac gaagctgagc
    17041 cggaagtcat tggcgaaaag tagatagatc aggatcgcgg cggccgaagt caccaacccc
    17101 cagatcgcca gcacacctcg ctccgcgctg agcgacagat acggtttctt cttccatttg
    17161 ccgaccagcg ccgccaggat tgtgtaaatg gaaaggcaga aagccagcag gagggctaac
    17221 gatccaaggt tttccataaa tttgcgccag gcttgctcga atcagctcga ccgctgcggc
    17281 tcttgccggt tggccgacgg ctggccgccg ggcatggctt catatttgga agcgcacttg
    17341 gcctgtatcc tcttcgcctc aaatactcca tcacggccga gtcgcccatc ggccagacac
    17401 tgcgctctgt ctttgaacgt atcgggcagg gggtctcgcc cggtatatac gactttcagc
    17461 cttcgattgt cctgcaaaag gacgaattct acccggtccg cgctgcgccg gatcgagttg
    17521 ggctccacgt cgcccgccac gcgcaggcgc ttgtccagcg ccttatcctg catcgcttgc
    17581 agttccgata cggttatgta gtaggttttg ctctcgttga tgccggccag agcgagccac
    17641 accaaggtcc cgagtacgat taccaccaag gctacgaact tgccatagcg tctcatcaca
    17701 accctccgcc gcgagtttcc gctgcgcagc tcggaacggc agccatggga aatctctccc
    17761 cgtagcgcga tatcaaacac ttagtatagc gcgtttagtc agcagcgatt ctatatcgcc
    17821 cgagccggac cggctgtaaa caaattgtgg attccgcggc cgtagaacag gttccgcccc
    17881 tcgaccagcg cacgcatctt tccgtcggcc acgttgcgcg gcagcatcca gaacccgcag
    17941 tccgggtgaa cccactttac ccgcccttcg cccagaacct cgaacgcctg ctcaatccgc
    18001 gcagcaatca ggtccggagc ctcaatttca ttgtccttga tatccaccac gcccagtccc
    18061 aggccgatct taggatcgag gtcgcgaaag gccgccagtt cctcatagcc gcggcgggcg
    18121 aattcgagca cagcgtgccc ggcgtgcaat ccattcagaa aaggcagcag gtttcgccag
    18181 aaacctttct gaacactctg accgccgtaa tttccgaagc agatatgcac ggcccgctcg
    18241 acggcaatcc catccagaac atgattgatc gcttccaccg cccaggtttg gtcctcgggg
    18301 tgcccgctga tattggcttc atccaactgg attaccgccg ggggggtggc cgtggagcaa
    18361 acggcggcca actggttccg cagtacggcg gccagggcca tcgaaagcct tcctggatcg
    18421 cgataatgcc ggtccacgag ggtcttggcg agcatatgcg ggccggtaca agtaaatttc
    18481 aaaggggaac gagtcaaagc actcgcgaat tcgaaatcac cgggcaggtt gagcgacccc
    18541 tctcctggct ccccgcggac gatcccggcg ggagccttcc ggaaccccat gccggcatcg
    18601 gctcggaagg cggcgagatc cgaaatcgag taccgtgtct cgacgcccga catgcggctc
    18661 acgaagtaat caatcatgcc gttggtttca ggatggctgg gatcgaagcg ttccaattcg
    18721 cccgaggcga ttacatcgag tccggccagc tcctgtgttt tcaataccac cataacggcg
    18781 tcccgcaacg ccgccctgga tggattccca ttgagccaga gggggatcgg atagctgccg
    18841 acaaccgtgg tttttatcat ggcaggtcga ttgaaattgt gtgtgaccgc gagatttctg
    18901 cccggttccc gatccagccg ggccgaatcg ccgccggcag ccccccgccc ggagccggca
    18961 tcccgcggcg cccggccgct ccggcgcggc gagtgccgtt ttttcctgct gtcaagcccg
    19021 gcctccggcg ttattatagg ccattatgtc gaggatcctt ctgcttctcc tcagcgctgc
    19081 cggtctcgcc gccggccagc aagtccagtt ccgcgcccat gtcattgaat ccaaaattcc
    19141 gggcggctac tcggtcattg tcgtggacgt gaatcacgac ggaaagccgg acgtgatcgg
    19201 aatgacgcaa caggtgcgcc agcttaaatg gtacgaaaat ccttcctggg agccgcacgt
    19261 catcatcgac gacatgactg gcatggtcaa tctagctgct tacgacatcg atggcgacgg
    19321 ggtccccgaa ctggcgattg aaagtggatt cagcatggta gccgccaaaa gcgaaggact
    19381 cgtgtggctg ctccagcacc agggcgatcc gaagcaactg tggaaagcca ggcggatcga
    19441 cgccctcacc acctcccacc acatagcctg ggccgatgtt gacggcgacg gcaagaaaga
    19501 gctgatcaac gctccgttga tcggcgcgac caacgtggcc ccgaagtacg agggcaaaac
    19561 gccgctggtt ttctatcggg tgccgaaaga ctggtccagc gattggaagc gcctgatgat
    19621 cgatgatcag ctcaacggca tcacacaccg ggtccgggtg gtgcattgga acggaacagg
    19681 caagcgcgaa caactgctga ccgcagggtt cgatggcgtg atcctccact ccgcttcggg
    19741 caaaggcgac aagttgcaat gggagcacaa gctgctcgta aaaggccacg atacggagga
    19801 ggctccgcgg ctcggaacca gcgatgtgaa agtcgcccac ctgggcaaac gccggttttt
    19861 agccgcggtc gagccctggc acggcaacga agtcgtcgtt tacaccgatg acggcaaggg
    19921 aggctggaag cgccaggtga tctacgatca gctcacggaa ggccacgaag tctgcgtcgg
    19981 cgacttcaac ggcgacggcc gcgacgatat cgtagccggc gaccgcgcca ggggtaaagt
    20041 cgccagctct cacatttttt attccctcga cgacgccggc gcgcgctggc ggcatgaaga
    20101 gctcgatcat ctcggcatgt cggcctcagg atgccaggtg gccgatatca acggcgacgg
    20161 ccggccggac atcgtcatga tcggcggcgc tacggcgaat atcaagtggt acgaaaatct
    20221 gggccctgcg aagtagtctc gcccctctgt acgggatctt ccaagcggca aacatcagcg
    20281 cgagctagcg ctgagggttg tcccagactt gccagcggcg gctggaggcg ggccgctttc
    20341 aggtatgaat cgtccaaatc tccgagaggg ccctttggaa attcccgccc aaaatgagct
    20401 cgatctggcg atccgtgtag cctcgccgga ccaggccctc ggttagatcg aagatcttct
    20461 tctcgtaatc gattccgtcc agatcgatct tttcgcccgg ccggattccc tgggcccgct
    20521 ggatttcagg tggaaggcgc gatcgtccat ccagatccac atcgcttccc acgccgacgt
    20581 gttccactcc cgccagccgc accacccact cgatgtgatc caataccgcg tccatcgtgg
    20641 ccgggccccc gttccggacg aagcttcgga tcatggtgat gcccatgact ccgcccttgg
    20701 cggccatctt ttgaatggct tcgtccgtct tgcaccggca gcggttcggg accagcgcgc
    20761 ggcaattcga gtgagtcacc agcacgggct tgcgcgacgc ctcaaaggca tccagcgtcg
    20821 tccggtcccc gcaatgtgag acatctatcg ccatcccgac ctcgttcatc cgctcgataa
    20881 tgcgccgccc aaactcgctc aggccgggat cgcgcggatc aaaagctccg gctccgatcc
    20941 ggttcgtgcg gtaggcgagt tgagaaacgc gctgacccag cgcaaagaag cggtttacgt
    21001 catcgaccga tcggaagtgt tccgaattct gctcgcccag cagaatgccg atcttgttct
    21061 gccgcttggg caccgcaaaa tctccggcgc ggtcgatgcg cagaaaatag cggctctgct
    21121 tggcaatgag gttattccac cggcgcatat cgagaagcga cgcggagtag atgtctccct
    21181 cgtcgtggcc cacggcgggg tggaaaatgg tgatgccgga gttttgcagt ttctcgaaat
    21241 ccggcggttg aaaggtctcc ggagcgagtt gccaggcgta gagtttcggg aaatccagcg
    21301 tgaccaaacc cagcatgtcg atcaccgtgg tgcgcccgac gagctcaatc gttcgcccgg
    21361 agtatgtagt ctcttcctgg gcaaacaggc ggaacctgcc ccggttgatc atgggcgcgg
    21421 ccgcaacggc gcccaacgcg cctgcggcgt tcttcaaaaa actccggcga gattgtttta
    21481 gggcagagat cgagccacga cttcggttac cagattcggc atccttcggc caacagcatc
    21541 ccttctcctc agcggtccca gccgccgact ggagcgaagt ataccatttg cgcgagcgtg
    21601 aaaacacgca gagagcctct aatcggaccg gccgaatagc gaggaaatac acaaaaggta
    21661 acccgcggcg cttgcgtcga tgcggcgccg tgtccggctg gaagcctcta cacccccgca
    21721 accaggtagc gtaagccgag atttacaagg ctcaggatga tgccgccgaa gaacgccggc
    21781 agaaacccgc ggatcgcaaa tccgggtacc agggccgcgg ccaccttcaa cattaaggcg
    21841 ttgataatga gccaaaagac tccgagggtg acaacggtga ggggaagggt gacaatctta
    21901 aggaaaaatc ccaatgtcgc gttgacgaag cctataacaa tggcggccac aagcgccgtt
    21961 cccaggccgc tgatttcgaa cccgcgaatc acatgagcca caatgagtag gctaaccgcg
    22021 ctcagaagcc agttcacaag gacctgtgcc atagtccgcc tcttatctaa cagacgtgcc
    22081 cgggttcacc agcgtagcac ccaaagcggc aatcctggta tgccaacgga ccagatcacg
    22141 gatcgaggca cgccggatcc gcaagctgaa atccggagag tatcgccttt actcccgaaa
    22201 aatcgatcca agaacaggcc ggcgcagaaa cctgggaact tttgcaacgc gcgaggccgc
    22261 cgagcagcac gagcgccaaa tccagttttt caagcgccgg gcgggcggcg gccattaaac
    22321 cgccggcccc ttgctctttt cgggctattg cgcgctccaa cccggcagcg ggacatgcgc
    22381 ctgctcgatg gcgcgctccc actcggccct ctcggccgcg ctccctgcca ctccatgctc
    22441 ggcaaagagg ccgggcaatc cacgcggctc gcagatccgg tacacctcgg taagctcgag
    22501 tcctgggatt gcctggacaa acggtatccg cattggcttg ccggcttgca ggctccgctg
    22561 cgaggcggcg tgaaatacgg cgtcccaccc gaaccagaag gccggctggt ttataggctg
    22621 cgcgaggagt tcgtcgcgat ggtgacgggc cacggaaagc tgcgagctca gatagcagag
    22681 cgctagcgcg gccgccgcca aggcgagtgc gagccgcagg cgcggccgct tttgccattt
    22741 ccgctgaagc ccgtcccaca cggctccgga gaacatcgcc caaagcgtga aactttggta
    22801 cgcataacgg ctgattttcc aaacctcggg ctccgcgcgg accagcatga tgacaaacag
    22861 gggaggcagc gtcaggcagg ccgatgccaa gacccagcgc aggttggtcc agctccacaa
    22921 cgcagccaaa atgagcgccg gaacggcgaa caagatcgag aatcgataag ttcccggcag
    22981 gacacgaatc gcgaggccga actgatagat ggacgaatgc agcgtgtctc ttgcggccca
    23041 tgccagccgc agcaggactc cggccgaagc gcccccggcg cgccaataat gccgcagatt
    23101 cgccgacctc ggaaccagcg ccagccagga aacggcgccg agcgtccatg cggcgccgga
    23161 cgcccgaagg gcccgggccg cggccgctgg ctcgagcagc gccacagccg caatcacacc
    23221 cggcaaagcg aaaagagctc ccgaaaacag gccaaccgag ccgagcaggg acgcggccgc
    23281 aatccactcc gacattccgg tgttttgcgt cagcagccag atccccagca acaggaacgt
    23341 ccacgagagc gcaaacggtg atgccgcttt ccaggctagc gcctcatccc cggccgttga
    23401 ccaggcgaag gacgccgcgg tcgccagcgc cgcccacggg ctgcgcaggt agcggcgcgc
    23461 cagggcatag agaagagccg cgcttgccag gtgcgcggcc tccaccaccg cgtggaagcc
    23521 ggcataccaa gccgggaaat gcaaatcgaa cggcaagcga agcagccgga acaggggcag
    23581 gacgtggatg tgctgcgggg tccaaagcaa tttccggatc tcagaccaat cgccagcgat
    23641 tttccggctg acgcccatca ggaggatgtc gtcgggcaaa atttcaaaca cctgtgcgta
    23701 gatccagtgc aggcgggccg ctgttaacag gacgcacgcc agcaacgccg gcgggatcag
    23761 ccgggagtcg cgcaccgaat gccaatacac ggagccatat gatagcagcg gccgctggcc
    23821 gccggccgga agccccggac cggaagagcg ccgatgtaaa atcaatcatg gctgccgata
    23881 cggccggcta cgtactggta ggtgggcgca gttcccgctt tgggtcgaac aaagcgtttt
    23941 tctcctggca ggggcgccct ttagcgcttc gggtggcgga acaggtgcgc caagccgcgg
    24001 gttccgtggt gctcataggc gatcctgaga catatcggcc gttgggactg ccggtgatcc
    24061 cggatccggt cagcggagca ggcccgctgg cgggcctggc ggctgctctt ggtcactctt
    24121 ccgccgcgtg gaatctggtg gttgcgtgcg atatgcctca cttgcgcgcc gattttctgg
    24181 agttcctgct ctcgcttgcc ttccgccgcg ctggggattg cgatgccctc gtgccggtcg
    24241 atcaggccgg gcgcccggag ccgctctgcg ctgtttatgc gaagcgctgc cagccggcga
    24301 ttcaagaggc cttgcttggc ggagtcagaa agatcactgg cgccttccgg aatctccggg
    24361 tgtacgagac gccctacgcc gactgggcgg catacgacgc cggcgggctg ctctttgcca
    24421 atgtgaacac ccgggccgac ctggacgcgg cgcacgccgc cccgtaaggg aaccatgccc
    24481 gaggaccaac agtaccctca ctacatcgag tttcgcggcg tatccaaggc ctttgagcag
    24541 cccgtactga tcgactgtag ctttcatctg gacagcggaa aaaccctgtc cattatcggc
    24601 cgcagcggag tagggaagtc ggtgaccctc tcccacctga tgggttttct caaacccgac
    24661 agaggccgcg tcatcgtggc gcacgaagat atcaccgact atccggagag cgagctgaac
    24721 cggatccgca agaaggtaac gatggtgttc cagtccggag cgctgttcga ctctctgacc
    24781 gtcgccgaga acgtagcgtt tcctctggag gaccaaggtg aaacagcgcg caacatcgat
    24841 gaggtcgtgc gcggtctgct cgatatggtt gaaatcgggc agtacgccga tatgtatccc
    24901 gccgagttgt ccacgggaat gaagcgcgcg gtggccattg cccgcgctct tgccgcgcag
    24961 ccggaagcga tcctttacga tgagccgaca accatggtgg atccgcccgc ctccgagcac
    25021 atcggggatc tcatccagcg cttgaagagc cagttgaagc tcacctcagt agtggtgacg
    25081 catgatcttg atttgatgcg caaggtggcc gacgatgtgg tgttcctgca cgaaggccac
    25141 gtgatattct tcggccctct cgatgagctg gagagcagcc agaacccaca tattcgtgaa
    25201 tttctgactc tcgatcgctt cgaagttccg gattaagaca cagcaggatt ccagattgca
    25261 aagccgccgg cgggtcctgc tgacggaagt gcggggcgat ctgccggaca gcggaggcta
    25321 cgcgcccttt ttccgcgcca gcgccggaga gggccggcgg aagaagcgca gggaagggaa
    25381 acgaatttgg ccggattttt aaaatctacc cctgtccgga tttgatattg gtgtgcgcca
    25441 gaggcgcggc ctctgcgggc gcggcgagga ccctatcggc ggctgatttc gcagtgcgga
    25501 tgcaatccgg aattccgatc ccgctatagg catttccggc caagtgaagc cagcggaaag
    25561 gcgccagccg gcgctgaatc tccgcgatac ggcgttcgtg gcccacgttg tattgggcca
    25621 tcgcgcgagg ccagcgataa actttgaaaa attcgggcgc cgccgctacg cccatatatt
    25681 gcgcgagctc ttctcgaact ccctcgacca gttcctcatc gttgcattgc agcagatcgc
    25741 cctcatttcc agcgccgata aagccgcgga gcaagacgcg gcgcccggtg acgcggttgg
    25801 gaaatttcgt gcccacccat gtgcatgccg taagcctgcg gcgctcgacc ttgggcgtca
    25861 gaaagccgaa gccgttcagc gggtgcgaga agtgagcgcg attgtacccg agagacacag
    25921 tcagcgaaga atgatatcgg acgccggaga gcaactcggc ggcgcatccc tccacggctt
    25981 gcagcaagcg gccggcgatg taggcgggcg cagccacgat gacgtcggaa gcctcgatcc
    26041 attgtccgcc gaagaaaatg cggcaacctt cgcccgtctc ctcgattcgc tcggcttcgc
    26101 ctcgaagcac cgcgacccgc ccggcgatgg ctcgcgcaag ggcgtcgacg agctgctgca
    26161 tcccgcccct gaggctgaga aaaatggcct gcggctcgct gcccgatccc gccgcctggg
    26221 cgcggcttgc cagcgccccg cgagtcaggc tgccgtagcg gcgctccaac tcgaccaggc
    26281 gcggtaaaac gcttctggcg ctcaggtcgg cggcgtcacc gccgtaaatg cccgccagga
    26341 gcggttcggc gagataatcg accgcctcgt ctccgtaatg ggcgcgaaga aagctggctg
    26401 ctgacacgtc cgcagtcgaa gaatcaaatg gggcgccgga ttctgcgtct tctcccggtc
    26461 ccggttcctc ggcccggccc gccgccgggc gccgcagcat atcagccgcc atccggagct
    26521 tggttcgcca gccgagcaag cgcgtagtga gtatggggcg catggctgtc gggatcatca
    26581 gatacaagcc ttccggcatg ggtatgagcc ggccccgccg cagaatgtag gtctggcgca
    26641 aatgatcgtt ggaaccgatg acgtcccgct cgattcccag ttcgcggatc aacgccagcg
    26701 cccagggctt ttgcgagaga aagctatccg gcccgccctc aacgacgcag ccctctatgt
    26761 gttccgtgcg aatcacgccg cccaggcgcg cttcgcgctc aatcagcgtg cagcggcatc
    26821 ctctacgaga aaggtaatag gccgccgcca agccggaaat gccgccgccg atgatgcata
    26881 catgcctcat cgcaagtgct cccgcgccac cgcggccagc gcttgaataa acagcggcga
    26941 atcattcagc gactccggcc gccgcagagt catgccgcgt tccgacgcgt attgccggaa
    27001 cacaatatcc acgtcgtaca ggatctcgac atgatcgcag acgaagccga tcggatgaag
    27061 cacaaactct ttcgcgccgg ccgcacacag cgtatcgatc cgcgactcca cagtcggccc
    27121 cagccatttc tcttccgtga gaccctggct ctgataagcg aaatcccagt gctgcaatct
    27181 tagctcggag gccacggcgg ccgctgtggc gcgcacttcg gcatcatagg gatcgccctg
    27241 cgcgagcact ttcgcgggca aactgtgggc ggtgaataaa acagggcagg aatgcgcgtg
    27301 cagagccggg cgcaggcgtt cggcaaaggc tgcgatcaac aacggatgcc ggtgaaaact
    27361 ttccacccag gagacctcga tgcggccgcc gatctgctcg agcgcttcgc gcgtgcggcg
    27421 gaagtagaag cccacgctca tcgtcgaata ctgcggagcc atgcacatcg ccacggcccg
    27481 cccgatgccg tcggccgcca tttgggccac ggtatcccga atatagggct tccagttgcg
    27541 catgcccacg tagacgggaa cccccagttc cgcctcgagc gccttggcct gccggagagt
    27601 gatcgccagc agcggcgagc cgcctccgat cgccgcgtaa cgctggcgga tctcctcgac
    27661 caccgcaggg gcgagcggcc ggccggcgcg gacgttcagg aggtattcgt cgatttgatc
    27721 caggctcacc ggcgcgccgt gagccagcag gaggaccgct tgtttcatcg gttttgccgt
    27781 atctgttccg ccgcttcagg aagctccata ttcatggacc atttccacca ccgccttgac
    27841 gttctccacc ggcgtctccg gaagaatgcc gtggccaaga ttaaagatgt ggcccggccg
    27901 gcgccctgcc cggctgagaa tgtcttccac ccgaatccgg agttccttga gcggcgcaaa
    27961 cagggccgcc ggatcgaggt tgccctgcac gccggcccga tagcggatcg agcgccaaat
    28021 ctgatccagt tccacccgcc agtcgagacc cagaacatcg gcgccggcct tgtgcagcat
    28081 cgggtaataa gatcccacgc cggtggcgaa gtggattacg ggaacgcccg cgctgcggac
    28141 gctgcggatc agagcgcgcg agtgcggcag tacataacgg taatagtctc caggcgccag
    28201 agcgccggcc caactgtcaa atacctgaat cgccgcagcg ccggcggtgg cttgcagtct
    28261 ggcgtacgaa gcgagcaccg agaccagctt ttccattagc agcgaccaca gctcgggcgt
    28321 cccgaacatc atccgcttgg tgtggagata atgcctcgac gcgccgcctt cgatcagata
    28381 gctgcccagg gtgaatgggg cgccgacgaa gccgatcacg ggaattttgc cgtcgagcga
    28441 gcggcgaacg agttggatcg cctcggcgac gtagccgaga tcgccgatat ggttcgactc
    28501 gagcgctgcc acgtcggcgg ccgtgcgcac gggattggca atgtgcggcc cttcgccggc
    28561 gacaaactcc agcttcaggc ccatcggctc cgcaggaaga agcaggtcgg cgaaaataat
    28621 ggcagcatct acgccgagag ccgccaccgg ctgtaaggtc acttccgccg caatcgccgg
    28681 ccgcttgcac atttcgagaa tcgtgtgccg cgagcgaacc gcccgatatt gctccatgta
    28741 gcggcccgcc tggcgcataa accaaaccgg cgtgcgctcc accggttccc ggcggcatgc
    28801 ggcttcgaac agcgagggca cgctttgaat atagccgatc tggcgactcc aaacgaggct
    28861 cctcgccgtg cggcttccgg cggctcacgc gcagtttttc cgctttcaga ccccctgtaa
    28921 gggcgagttc cctatttctt ggccccgact acggagcagc gatacagctc tccagtgggc
    28981 gtgagggtga tctcgccgct ttgagccgcc tcaacaatct ggccatctga ggtgaactgg
    29041 cgcggcgggt tgctgctgtt gaatgtgatt tgcacttccg cccacagggg attgaagccg
    29101 tcgccttgga tcgcgtcgat caccgcgata aataggaaac ccgaagcgcc caattcgcac
    29161 tggtcgcaca tatagatcgt gttgattgag ccgttgtgtt gcaggttcgc gttttctccg
    29221 ccggacggca actgcttgaa gttgatggtg aagagttggg cgtcgtagta agccggcatg
    29281 tcgccggccg aaccattcag gctgccggcg aaagcggccg cgccggcgag caggccacaa
    29341 gcagccagga agccgagacg ctggaccgat acattcataa gtcccccttt tgtgccgagg
    29401 ctagcacgcc ggtagttgcg cccaacccgc agaacccgct tctttacacc tgttgcgcta
    29461 ccagatgtgc gcttgcccac cgcttgcaat atcgctttgc gaaaagcgaa agcgcctggc
    29521 tgaaagcctc cttcagcccg agaaggtatt ggcggatgtt tcgtctgctt tctcacccgc
    29581 gcccgcgtcg gggtgtgaaa attcgcatcc aatgtaatgg ctgaggtttt atgaaacgca
    29641 actggaagca agttgggcga accgcgccgc tattgctcct tgtgtttgtg gcggcagggc
    29701 ttcttttcgc ccaaacagca tctatgcctg cgccaggcgc cctgaattac atcgagggcc
    29761 aggtttacgt ggatgggcgg ccattgccgc caggcgccgt gcgttccacc gtgcttgcgc
    29821 cgcatcaggt gttgcaaaca ggccagggaa aggcggaatt gcttttgacg ccgggcgtgt
    29881 ttctccgcgt cggtgacggg agcgagatgc gcatgagctc ggccggcttg gctgatgtga
    29941 aagtggcggt gattcgggga tcggcaatgc tggaagccgc cgaactgttc aaggagaaca
    30001 acctggcagt ctcggtcggc gaggcgaccg ccaaggtgga gaagaaaggt ttatacgaat
    30061 tcagcgcgag ccagccggca attgccgtcc tagacggcaa ggcagttgtt tatcaggacg
    30121 actcgcatgt tacggttcag aagaacgagg aggtcttgct ggagcagcat cctctaaagg
    30181 cgcggaagct cgacgaaaag acaatccagg ccgatcctct ctatcgctgg agcaagctgc
    30241 gcagcgagta tctggcgagg gcgaatatcg acacggccag gactgtggtc atgtacgggg
    30301 gctggtacgg tccgggctgg tattgggacc ccttctgggg tttctattcc tatgtgccgg
    30361 cctggggcgt catatacagc ccgttcggct tcgcgtttta cgcgccggct tgggtttgga
    30421 acgcccccat agtgtattac cactatcccg tgatagcggg ctcttactac cgccggcggg
    30481 tccccggcag aggcttccgg agtgtgccgc gtatcgcgca cgcctttcac ggtgttccgc
    30541 ccggcgggtt ccgggcggcg acgccgagaa tcccgcctcg cggtattgct ctcgcgagtg
    30601 gcagcttccg ccccgggggt gtttccggcg gcgccgggcg ccgttgagcc tctgcgcact
    30661 cccgcccctc gagctggcgt gcgatctccg aaagatccat cgcaaacaca acaactccga
    30721 tgccgctccc caatccctcc aggtaaaaaa agatgtaaaa agatttcggc gggccgccaa
    30781 tgattgtcag ggagcggcaa ggggtgtatg ctagcaacgg tcgcatgttt ccggcacgct
    30841 cggcggttgc ttcctgctgc tttttgtcgt tctcggtcct ggcaggttac gcgcaaggtc
    30901 ctccggttgc tgctccggac gccggttctt atgtcgggtc ggcggcctgc aaaacctgcc
    30961 acgcctccat ctacgcccga tgggtcaaga cccgaatggc gaacgtagtg cgcgacccgc
    31021 gcgaacatcc ggacgccatt attcccgatc tttcccggcc cgacccgctc gtcaagttct
    31081 ctaaagacga tattgcgttc gtttatggca gcaagtggaa acagcgctac ttcaagaagg
    31141 tcggcgatga ttactacgcc tttccggccc aatgggacgt cacgcaccgg atctggaggc
    31201 cctatgtggt ccaacccggg actgactggt gggttcgctt ttaccctggc gataatttcc
    31261 agcgccccac cggccctctg tgcgacggct gccattctgt aaattacaac atcgccaccc
    31321 acagagtatc cgaatggaat gtgggctgcg agaaatgcca cggccccgga ggcgagcacg
    31381 tgaaacggcc gttgcgatcc aacattgtga atccggcccg gctagactac gtgcaggcca
    31441 acgacgtctg cattcagtgc cattcgcaag gccagccctt gaataacccc atccagggaa
    31501 aatactacga ctggcctgtg ggctttcggg ttggcctgag gctgagcgat tactggaagc
    31561 tcgaggagca caagctcggc gaaacctcat tcacccattt cgccgacggg accgcccata
    31621 agaaccggat gcaaggaaat gatttcgtga cgagcctgat gtatacgcgc ggggtcgcct
    31681 gtttcagttg ccacgatgtg cacggcacgg gcaataacgc cgatctggtg aaacccgcca
    31741 gcgtgctttg cctggaatgc catggcccca actctccgaa cgggccgcac gcctccagca
    31801 tcgagcagca cacgcatcat aaagccggca gttcgggcag cgagtgcatc gcctgccaca
    31861 tgccgcagat ccagcagacc atcgcggacg tgaacgttcg cgcccacact ttccgcttca
    31921 tcactccggc caagaccgaa actctgaaag tcccgaatgc ttgcaatgcc tgccacaaag
    31981 acaaatctac cgcatgggcc tcggaagcct tgaagagctg gcgcgaccgc tcgccctggc
    32041 gcatggccga gtgacgagtt gcagcaacag ttcggcggct gatcgacggc ccgcgccgcg
    32101 gcttggcgtg ttcgctgcct cgcaagccgg gatcgtgtcg aactggatgg ggaacagccg
    32161 ggccaggagc agatgatccg ccgcagcgct gacggcggga taccggcgga tttaaggatc
    32221 aggctctccg ctaagcccgg ttggactgcg atgcaggggc gagaatgtcc gtcagcctct
    32281 gggcgatgat tttctcctcg cccggcttca gcgtattcga aacaatcgaa atgagaggtg
    32341 ccgggcccga ctgcaccccc tgctcgctga gcatcgccga aaccctgggc agcacgattc
    32401 cgcgctcctc gccgattacc tcgatctgcg gcttgccgcg ccgcagcgca tcgatgcact
    32461 gctgccgggt cagcttgaat tctttttcgt cccagcgtat gcccacatgg ggcacttcat
    32521 tgccgagccg ccggtaaaaa atttccgcct tcacgcccgg aatttttgac agggccgtaa
    32581 cgatctgccg catctggatc tcccagaccc ggcaatccgc cgcgtagttg cgcttcgacg
    32641 accattgcac ggccgcgagc acgccgatga tttcctcctt gccgactttc atcggccggc
    32701 aaatcgaacc ctcataaggc gatccgttcc gcagcgctgc ctcgatcagg tgagaacggc
    32761 cgaagaggat cccggtgcag tcggggccgc gcagaccctt ccctccgctc acgcaaaaca
    32821 tgtccggacc gaggcttgcc agaaatcgca gacgctcaaa aggcggacac atggccgcgg
    32881 cgtccagcag aaacggcact ccgtggcgct tgcaaatcga gatggcctgc tccaggggca
    32941 ccgggccggg gtcgccgtat tcgaactcca tcatagcggt gcgctcgtta atcgaccgct
    33001 caaggtcggc caagctgctt acgactacca gcttggcgcc ggtcagcgcg atgctccggt
    33061 cccaaacgct gcggcgggtg ataatgacct cgtttttcat cccggtggtg tccggcagcc
    33121 gcgcgatttt ttccggatcc gtgcccgcca tgcagccggc cgtggcctgt gcgatgcagg
    33181 cggcggctcc gctcgatacc atggcgtctt cggttccagc gaaacgcgcc agccgctgcc
    33241 ccacttcctt ctgcaattcc acgatttcca cgaagtattg cgaggcccgc cgcatcgcct
    33301 ggtgcacttc atccggcatc agtgtgctcg aataataggg atccacgccc cgggcgttta
    33361 tcaatgtccg cactcccagt tcggcgtaga tgttgtctgg agcggcctga acttgggccg
    33421 tttgctgccc ggccagccgg gcagaagcgc cggccgccaa tccggcggcc acagggctga
    33481 ttcctgcgcc ttgcagaaag cgccgccggc tccaagccga ttttctattt ttcataaagg
    33541 ctgctccgtc ggtcgaatta tacacccgtt gcactgctat aatccggtgg gaggcccgcc
    33601 atgcggccct ttcgggtttg tagtctcatc ctgatcgtct tatctatttt cctgctgtgc
    33661 ctgcctgctc cttcccagcg caacgccgaa tatatgtggc cgctgcgccc cgtcgtgcgc
    33721 ggcactcgag gggccgtcgc cggtggaact tccatgggca ccgacgccgg aatgcggatg
    33781 tttcatgccg gcgggaatgc ggtcgatgcc ggagtcgccg cattgttcgc aggggcggtg
    33841 gtcgagtatt cgcacttcgg cttcggcggc gaagccccga tcctgatccg caccaaagac
    33901 ggccacgtgt actccatccc gggcgtcggc actgctccga aactcgccac gctggactat
    33961 tttcttcacc gcaagctttc cccggaagaa aagatgaacc cggacgcgga cgaacgggaa
    34021 ggcccgattc cgtcgttcgg gattctgccg gcgctcgtgc ccggcatggt cgacgccggg
    34081 ctggtggctc ttgagcgctt cggcacgaag agcttcggcg agataatcca gccggccatt
    34141 gagctggcgg acggcgctcc gatcgacgaa acgcgcagct ccgccatcgc ccactcggtc
    34201 cgctatttcc agaagtggcc gacgtcgcag catgtattca tgcccaacgg ccgcgttcct
    34261 cgtcctgggg agatctttcg gcagccggac ttggctcgca ccctgcgcgc tatggcggca
    34321 gccgaaaaaa gcgccctggc gcgccataag ggagaccgcg tcgccggcat cgaggcggtg
    34381 cgcgattatt tttatcgcgg cgaaatcgcc cgaaagattg acgccttcat gcgcgccaac
    34441 gacggcttgc tgcgctacga agacatggct tccttccgca tcgagcccga agagccggtg
    34501 cacaccacat accggggcta tgaagtctat aagagcggct tctggtcgca agggccggcg
    34561 ttcctcgagg ccctgaacat gctggagaag ttcgacctgg tcggcatgcg ctacaactcg
    34621 gcggattacc tccataccat ggtggaagcc ttgaaattag cttacgccga ccgtgacgcc
    34681 tactatggcg accccaagtt ctcgccggcc ccgatggagc ttttgaccaa agagtacgct
    34741 gcgcaaaggg acaaactcat cgatcctcat ctggcatccg cagaattccg gccgggggaa
    34801 atcagcctcc gaaagccgat gcacccttcc gcctacgccg gccggatcaa tcccctgagc
    34861 gacgtgctgg ccgcccacga caccacttgc ctgaacgcga tcgatcgcga cggcgtgatt
    34921 ttctcctcga cgccttcggg agcctggctg ctgcctgtct cggtgatcgc cggcgacacg
    34981 ggcattccct taacccagcg cggccaaagc tttttgctga ttcccggtca tcctaatgtc
    35041 atcgagccgg gcaaacgccc gcgcgtgacc ctgtcgccaa cgctggtgac gcgtcagggc
    35101 cagccctata tggctctttc cacccccggc ggcgacaatc aggagcaaag cctgctccag
    35161 attttcctgg acgtcgtgga gttcggcatg aacccgcaat tggccgtcga ggctccgcga
    35221 ttccagaccc ggcatctcgt ctccagcttc gacgcccatg cgacgcatcc caacagcctg
    35281 ctgcttgatg agcgcattcc tacggctgtt ttccgcgagc ttgccgcccg ggggcacagg
    35341 gtggagcggc ggcctgcgtg gcagaacgga gcggcgccga ccgccattcg gattcttccg
    35401 gaaaccggcg taatcgaggc cggcgccgac ccctatggta tgcgttacgc ggatgcctgg
    35461 taacgggcgg cgcgccggtc cgaggattgc cgccaggcag ccgcaggccg gctgatcgtg
    35521 cgcccgggcc tccgggcgtg ccttcatttc atggtaatga cggctttccc cacgccatcg
    35581 tcataatttt cgaggagttg aaaagccgcg gcgatgcttt cgagcggccg ggtgtgggtc
    35641 acgatctcgc cggcgcggag ccgccgctcc accagaagcc agattgcggc ggcagcgttg
    35701 cgattctgcc gccggacgtt gaataaagcg agctctttcc ttcgcatccc gtgaaactcc
    35761 acaggcacgc tcagttcggc cggaatgccg atgagcacga cgcggcccgc attccgcgcc
    35821 acccgcaagc agtggttgat ggcttccgcc ttacctgccg cctcgaacgc gacatccacg
    35881 ccgcgcccac tggtttcggc cagtatggcc ttatcaggag gcacggctga aaaatcgatg
    35941 gcgcagtccg cgcccacctg gcgcgccagt tccagccggg cgggaatgcg gtccacagca
    36001 tagatccggc ctgccccgca caacctcgcg gctgccattg tgagaagacc aatcggcccg
    36061 gcgccgaaca ccgccgctgt ctcgccgggc cgcagatcgg ccaatcgcat tgcattcaac
    36121 gctacggcca gaggctcgtg aagcacggcc tcttccatcg aaatcgtacg cggcaggggc
    36181 gttacgttcg cggcgggcaa attgatcact tcccggaaga aacccggttc cccggggttg
    36241 ctcagaaacc ggatccgctc gcaaacattc tgacggcccg caagacaata ctcgcacctc
    36301 ccgcaataga tcgccggttc caaagccgcc gccatgcccg gctccaatcc ggttacgccc
    36361 gcaccggctc gcagcacctc gcccgcgggc tcgtgcccca gaaccatcgg gaagatgcac
    36421 ggcgtatctc cgatgcctcc ttcccagtaa cagtgcagat cggagccgca gatgccgacc
    36481 gcccgcacgc gtacctgaac ttcccccggc ccgggatcgg gcgggtttga aatttccgcg
    36541 acgcgcaact tgcggacacc cacgagttcg gcggccagca taggcgagtt tcatcatatc
    36601 aggcggcgaa gccggtccaa tttcggtaaa ctgtccggcg gcgctgtttc cgcggtgaca
    36661 cgtgtccaag cgcaaggtca acgaatctat aaaagagttt cgcgttaagc cgggcgccaa
    36721 agtgcggctt aagaactttg atccagcccg ccactggagc ccgcgccacg accccaaact
    36781 ttccaaggct ctggcagacg atatcggccg catggccgaa ctggaggtgc gactctacgc
    36841 ggagcaccgg cgctccttgc tgatcgtgct gcaaggcatc gatgcgtcgg gtaaagatgg
    36901 gaccatcaag cacgtcatgt ccggtctgaa tccgcagtcg tgccgggttc acagtttcaa
    36961 gaagccggca ggggaagagg cggaacacga ttttctgtgg cgcattcatc gcgctttgcc
    37021 gggaaaaggg gagatcggca tcttcaaccg ttctcactat gaagatgtgc tcgtgccgcg
    37081 cgtgcaccag ctcgtgccga aagaagtttg ggagcaacgc taccagcaga tcaataattt
    37141 tgagcaggaa gtggccgaaa ccgggaccga gatcctgaag tttttccttc acatctcgaa
    37201 ggacgagcag cgccagcgtt ttcaggagcg cctcgacgat ccttccaagc actggaagct
    37261 gagcctgccc gacttcgaag aacggcgcct ttggggcgcc taccaggaag cattcgagga
    37321 catgctctca cgatgctcta cgccctgggc gccctggcac gtcgttccgg ccaaccataa
    37381 gtggttccgc aattatgttg tggcagggac gattgttgac aagctcgagg agatggatcc
    37441 gcagtttccg cccgtgacca tagatatttc taaaatcgtc ttgcgatgag ttccctcgct
    37501 cggccctggc agcgaattga ttctttccgg ctcagggact gcggccgcct ccatcttcat
    37561 gcttcaattg tgctgtggct gctcctgacg ttctcccccg cctggttctg gcgaagctcc
    37621 gggagcagat cgagcggact gagcacctga tcgcattgat cccgccggac aaactcgggt
    37681 ggaagcccga tatccccggc gcgcgctcaa tagcagaact gctgggccac ttgctggatt
    37741 gcctggcagg cttctgcgcc gctttgtgcg cgtttgaccc ggtgcgcctg gcccgcttcg
    37801 ccgatctgcg cggccgtaag gtcaatcatg cctgcgggac cgcggaggcc ctcgatcgcg
    37861 tgcgggaata cgcgggctgc atcgaagaag gtttcgcgtt gctcgccggc caggacttgg
    37921 tgcggcccgt gcaaacggtc ttcgtgcctg agggcgagcc gctgttgacc ctgctgctcg
    37981 gcaacctgga gcacttgatc aaccacaagc accagctctt cttctacctg aagctgctcg
    38041 gagcgccagt gagcacagcc gacctctacc gatttcgcgg ccccgcccca gcagcggagt
    38101 gacgcgccgc aaagccagct cttcgtttgc cctgatttcc ttgtaatgta tcacgcttgt
    38161 cttgcttgcc ccttttcatg aatgcatcct aaagacgggc ccgagcggcg gcgctgcgag
    38221 cagactgcca aactttttaa tgaacctgcc catccctcgc gtcagcggcg ccgccatagg
    38281 caggctggtg ggacgcaggg ttgacggatg aaggcggaca caaaggcttt cctgaataca
    38341 tccgaatact tcatataagt gtttttcgag ggatttgcga cggcccttgg cgggcgccga
    38401 tataaagctc aatctccggc cctgccggat ttttcgagct gttgtaaagc cggaatgtcg
    38461 tgctacaatg ctgccaccca aaagggccgc tgaggaggtg atatgcggtt ccaaccgaag
    38521 ttccttttgc ccgcctgtat ctggctgttt atctttcccg ccgcggcgca agtcgatcgc
    38581 gcagccatca acggaaccgt gaccgatgct agcggcggcg tcatcccggg cgccagggtg
    38641 gaggtcgtcg ccctgggtac cggcctccgg cgtgaaatgc tgacgggtct caacggcggg
    38701 tacgacatcc ctgggctgcc catcggcagc tataacgtca cgatttcgaa ggaaggtttc
    38761 agagcggtcc agttcaagga tgtctcgctt ttcgtcggcc aaacccgcac cctggatgcc
    38821 caattggatg tgagtgcggt ggcctcgcag gtcgaggtgc aggccacggc agccgcgatc
    38881 aatcgcagtt cggctgagat cggcggggtt atcgagtcgc agcaggtgcg ggacatcccg
    38941 ctgaacgggc ggaactgggc cagcctgatg acgctggcgc cgggggcgat caacgtgggc
    39001 ggaggggacc agcgaagtat ccggtttgtg ggccgctcgc gcgacgacaa caactacacg
    39061 tttgacggca ttgacgccag cggcgtgcag gagcagccgc agaagtcgga cgtgcggctg
    39121 aatatttcac tcgagtccat cgccgagttc cgcgtaaatg ccgcggcgta tacagccgaa
    39181 agcggcgccg ccggcggagc cgagatcaac gtggtttcaa agaccggaac caaccagtgg
    39241 cacggcgggg cgttcgagtt tctgcgcaac gacaagctta acgcccggac ccccttcgac
    39301 ccttcccagt tgccgccctt ccatctgaac cagtttgggg gcagcgtcgg cgggccgatc
    39361 gccagggacc ggacgttctt cttcgtaaat tatgaaggct tggagcagcg gctgggacag
    39421 acgttgatcg gatttgtgcc gagcccctcg ttccgtcagc aggtcatcgc cgcctcgcct
    39481 gcgctgcaat cgatgatgca ggcctatccg gcgggccaga cgcccgtgga tgccaatacc
    39541 tatcagttga ccgtgcaagg ccgcaactcg ctccgcgagg acagcggcat ggtccgagtc
    39601 gatcatcgct ttacggacaa aacttccatg ttcgcccgct acaacgtgga cgacgggctc
    39661 gtgcaatcgc caagcggcgc cctcggggcc aaggatacta tcgccatccg cccttcgaac
    39721 cttgccctcc agttgatgca cattttttcg cccaccataa tcaatgaaac caaggctggt
    39781 atgaaccgtt cggcgtacgt tcacactgtt gtggggatct tcccgcagga catcagcgct
    39841 cctggattcg atgacttgcc aaacagttcg ctggacgagg aaatcggcac cacttttact
    39901 tacgccgaca atctgaccat tatccgcggg cgccacacct tcaagatggg cgcggacatc
    39961 cggcgcgtcc gcctcaacaa ttccggcaac gccatcgaca ctacgacaat ctcgtatgcc
    40021 agcctgaaag acttcatcaa caatgccgtc gactccatca gcgtgaatgc cgcgctgggc
    40081 atcggcggga tgcggcgcac tttctggatg ggctatgggc aggacgagtt caaggcgcgc
    40141 cccaacctca cgatcaatct cgggctgcga tacgaatact actccgtgat gcatgaagtg
    40201 aaaaaccggg ctctggtggt ggatatcctg ggctgcggag gcttttgccc ggtcggcacg
    40261 ccgttttatt cgccggaccg cgacaacttc gccccgcggg tcggcattgc atggtccccg
    40321 tcaagcctta aggggaagac ggtcatccgg acgggcttcg gcatttactt tggcgcgaac
    40381 cagaatgacg acttcagcga tccccacgaa agcacagcag gccggtttgc tctctcctcc
    40441 gccgacgtgc ccaatctttc ctatcccatc actccctttc tgggccaact ccaatcccag
    40501 gggctgagcc cgaagggcat cgaccggaac cgccaggatc cctattacga aaactgggat
    40561 ttcctgatac agcagcaatt gccgcgcaac tttgtaggcc aagtcggcta cgtgggcagc
    40621 gaaggccaca ggctcttcac ttcgcgggcc gtcaatctga tcaatccatt gaccgggaaa
    40681 cgtcccttgt cgcagttcgg ccaattcaat gtgaagagcg atgacgcgaa cagcaacttc
    40741 aacgcgcttc aggcgtcgct acagcgttcc ttcaccggcg gctggctatg gcagacgcaa
    40801 tatatgtggt cgcacgctat taccgacggc tcggtcgggg cgggcgagag cgtggcggtg
    40861 cagaacgctt cctgccgcgc ctgcgaccgc agcgactcgc cctacgacgt gcgccacacc
    40921 atcaccataa attcggttta ccagttgccc ttcggccctg gtaagccctt tttgcacgcg
    40981 gagagtctgg ctggcaagct gatcggcggc tgggaactga gcgggctagg cgccgccagc
    41041 accggacggc ccgtgaacat cctggtcacg cggagcgcgt caagtttgct ggacggcaac
    41101 cgatcgaacc agcggccgga cctggttccg ggcgtgccga tttaccccgc cagcggccgg
    41161 agcatctacc agtggttcaa cccagcggcg tttgctgtgc ccgcgaaggg cacgtggggc
    41221 aaccttggac gcaacatcgc gcgagggccg ggcctgtggg aaatagatac cgcgctccag
    41281 aagcggattc cggttttcga gcgcgcgaac ctgagcttcc gcgccgaggc cttcaacctc
    41341 ttcaaccatc ctatctttgc caatcccgcg gccaacattt cgtcctccag cttcgggaga
    41401 atcacgagcg tgctgaataa cggggcggtc ggcacgggcg cgccgcgccg gctgcaattt
    41461 atgttaagac tggatttctg agctctgaaa aatgaccacc tttcgcgggg ccgttctggc
    41521 tgccctcatc ggcctgagcg ggatcctttg ttctgcgcct tccccccagg gcgtcgatgc
    41581 agcgcagagc ccgtttctgg taaagcccta cttgcagctc ggcaactacg cccagccgtc
    41641 caatccggag cgtctggctc tgctgtggca taccgcaggc gccgatgccg actggcgagt
    41701 ggaggtgaag tcatccgcag cgaatgagtg gtccgcctgc gactcccctg ttgagcggcg
    41761 catcgcggta tccggcatcc ccccgcatcg ggtgtacagg gccgtgctct cgcggctgcc
    41821 gcccggagaa gagttcgaat atcgggttct gaaaaacgga aaggaagtct tccgcgcaca
    41881 ggggcggagc cgcaaacctg tcggtcggcc ttaccggttt gtggttttcg gcgactgtgg
    41941 cgcggggacg gcttcgcagg ccgcggtggc ctatcagatc ttccaggcca agcccgattt
    42001 cgttgttacg gcaggcgata tcgtctacgg caatgggaga atttccgaat atcggacaaa
    42061 attctttccc atttataacg ccgatgggcc ctccatgacc ggcgcaccgc tcatacgatc
    42121 cacactgttt ctcgccgcgc cgggaaatca cgacatgtcc aaccgttttc ctgacgggct
    42181 ggcgtatttt ctgtactggg accagccgct caacgggccg atcgctgagt ctagttccat
    42241 gcatgcgcct gcattgccgg gtgacgagga ccaagcggcg tttttgcggg ctgcagggtc
    42301 aacatacccc cgcatgggaa atttctcgtt caactacggc aattcccatt ggaccgtgct
    42361 cgacagcaat cgttacgtcg attggaactc ccctgcgctg cgcgcctggg tggctgggga
    42421 cctctcggct gcgcgccgcg ccgcctggcg cttcgttgtt tttcaccatc cgcccttcca
    42481 ttcctcgcac aggcatttcg aagatcagtg gatgcgggtg ctggcggatg tctttcaggc
    42541 cggcaacgtg gacattgtct ttgccggaca tgtccacaac tatcagcggt ctttgccgct
    42601 gcggttcacc ccaaaagccg gctcggacgg caatcttctt gcccgcagcg ggcgcgtgga
    42661 tggcgccttt gcgctcgacc gcaatttcga tgggaaagcg cgcaccaggc ccaatgggat
    42721 tctttacctg gtgaccggcg cgggcggcgc cgggctgtat gaccccgatc agcagaatga
    42781 caggtcgtca tggcaggagt tcaccgcccg atttttttcc aaccagcatt cgttcaccct
    42841 ggtgaatgtg gacggcaaga ggctggacgt aacgcaggtg gcggaggacg gcgccgtact
    42901 ggaccagttc accgtgtcga agtaagacac gttctacagc ggtttcgcgt gaaaatctag
    42961 gccgcctggc tggccgcgat ggccggcgcg ctttttgacc ggtttacggg tagattatca
    43021 ggcggcggat gccgtactcg ccttccttgc tctggcgaca cgcgccaagc ctgtgttcgc
    43081 cctgggtggc gccgcctgtc gtccggcagc cgggcgcatt cgccacaccg gccgatccac
    43141 gagtaacggc tctcgcacgc ggagcgcctt tatggtacaa gagacttagt atgcgatcga
    43201 taggttttcc ggagttactg atcattttcg ggattgctgt gctgttcttt ggcagcaaca
    43261 agatcccaga tcttgccaag ggcctgggcg aagggatccg caacttcaag gcggccataa
    43321 agggcgacga gcccccgaaa gcctgacgcg gaactcgcgc tgcccagttc ccccaagcca
    43381 tggtgcgggg ggtgcggcgc ggcctgcaag acggagatcg tgcttccccg ccgagttcat
    43441 cggcccgagc gggctgtgtc tgacaatcgc cgggcctgat gcgcccgagc cgcacttcgt
    43501 atcacactta gaatttctat gggcggaccc ccgggccgga aacactttct ttcctccgct
    43561 tgtcagagaa gccggaacgc cgtctcagcc tatcagagac cagtgttgac cggaccgccg
    43621 tctgagacgg gataacagca cccgcaggcc gccggccggg cgaagcgcgg ggagcctcgg
    43681 gcggcgccgc gtccacgggg cgtccgcaga tgcaaccccg cggcgtgcgt gctagtatca
    43741 agaacatttg gaggcgaact catgagcaaa tccgcgcgca ctgtgctggt actgcttctg
    43801 gttatggtcc tttgcggcgg cggcctgctg agcgccgcta cgcgcccgat cgtcatggga
    43861 actcatggca tggcggtgag cggccatccg ctggcggcgc gcgccggaat gaaaatgctc
    43921 gagcagggcg gcaacgccat agatgctggc gtcgcccagg tgttcgcgca ggcggttctc
    43981 gagttcaatc tgtttggcat cggcggcgaa tgcccgatcc tgatctattc cgcaaaagac
    44041 aagaaggttt acgcgatcaa cggcc
//
LOCUS       DASREK010000188         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1740765, whole genome shotgun sequence.
ACCESSION   DASREK010000188 DASREK010000000
VERSION     DASREK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740765"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFA54_09770"
     CDS             complement(<1..942)
                     /locus_tag="VFA54_09770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZS51137.1"
                     /translation="MTELEREIRSEIAARGPMSFERFMSLALYHPKHGYYRSKHDPFG
                     VAGDYYTSSQLQPVFGRLIADQIARWRVQAGGAGFTAVELGAGRGETAREIRSRLPGV
                     PCIEVEHQMGDLPEHMTGVIFSNEFFDALPVHVVRCGEQGILERFVGLLEGKLTWVEA
                     EPSSPAIVEYVQRFAPALARWQVIEVNLEALKWTERIASSLDRGWVVTIDYGYTADEI
                     AGGKRFPEGSLMSYYRHSAYEDVLRDPGERDITAHVNFTALAEYGASLGLRALPLQTQ
                     AQFLLSIGQADGFESAVAGLTQAEIERHRLQLKTLLYG"
     gene            complement(939..1211)
                     /locus_tag="VFA54_09775"
     CDS             complement(939..1211)
                     /locus_tag="VFA54_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51138.1"
                     /translation="MSLLEQVRQQIARYEHPLFDFSARERAGRIELVIDCKVKNLGLH
                     TYYLELHPRDLESTQFPWTFQRLLYDGMNDYVVEMFTRTPQSRNEK"
     gene            1373..1768
                     /locus_tag="VFA54_09780"
     CDS             1373..1768
                     /locus_tag="VFA54_09780"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HZS51139.1"
                     /translation="MRREAFEQAVREAWLAIPAKFRQRVHNLAIVVEDEPSPELLRQN
                     GLPPGYALLGLYHGVPLIHRGASYGMVLPDKISLFQGPIERAAQRADDIREVIYDTLW
                     HEIAHYFGMNEAQVRAAERKRASRRRFRR"
     gene            1857..2963
                     /locus_tag="VFA54_09785"
     CDS             1857..2963
                     /locus_tag="VFA54_09785"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HZS51140.1"
                     /translation="MQRRKFVQLVGMGAAGAASAVPADAAARAKKGASPRRALMKVGT
                     QHGDSDEILRVLAALGVNNICSALPSPRFDEKWSAEGLTRLRERVESFGIKLDMVPLP
                     LSSSYITRSENPNIMLGKSPERDREIDNICQMIRNAKQAGIPALKYNMSILGVVRTER
                     TPGRGGASYSTFVYDKAKQEPPLTEAGPVSDEQAWERITYFLKRVIPVAEEYKIRMAC
                     HPHDPGMPRDRGFRGVHRVLGSVEGLKRFIEINPSPYHGLNFCQGTVSEMLENPGKEI
                     YDVIRYFGSRKKIFNVHFRNIRGKFLNFQETFIDDGDVDMLQAMRVYKEVGYDGMMMP
                     DHVPQIQGDAHGYQAFAYTFGYIKALIRAVDAEG"
     gene            complement(3014..>3251)
                     /locus_tag="VFA54_09790"
     CDS             complement(3014..>3251)
                     /locus_tag="VFA54_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZS51141.1"
                     /translation="TGGPGFVQASGASYREIFDLSDWDRSVAINAPGQSGQPGSPHYA
                     DLLPLWADGEYFPLPYSRAAVEKNTARRLVLEPK"
BASE COUNT          654 a         1019 c          976 g          602 t
ORIGIN      
        1 gccgtagagc agggttttga gttgcagccg gtggcgctcg atttcggctt gagtcagccc
       61 ggccacggcc gactcgaatc catccgcctg gccgatggag agtaagaact gcgcctgcgt
      121 ttgcaagggc aaggcgcgca ggccgagcga ggcgccgtat tcggcaaggg ccgtgaagtt
      181 gacgtgagcg gtgatgtccc gctctccggg atcgcgcagc acatcctcgt aggcgctgtg
      241 ccggtagtag ctcatcagcg atccttccgg gaaccgcttc cctccggcga tttcgtcggc
      301 cgtgtagccg taatcgattg tgaccaccca gccgcgatcg agcgaagaag cgatgcgctc
      361 ggtccatttg agcgcctcca gattcacctc gatcacctgc cagcgcgcca gcgccggagc
      421 aaatcgctgc acatactcca caatcgccgg cgaagaaggc tcggcctcca cccaagtcaa
      481 tttcccttcc agcagcccga caaatcgctc caaaatgcct tgctcgccgc atctcaccac
      541 gtgcaccggc aaagcatcga aaaactcatt ggaaaaaatg accccggtca tatgttccgg
      601 caggtcgccc atctggtgtt ccacctcgat acagggcact cccggcaacc gggaccgaat
      661 ctcgcgggcg gtttcgccgc gccctgcgcc caattcgacc gcggtgaacc cggcgccgcc
      721 ggcctggaca cgccatctgg cgatttgatc cgctatcaaa cggccaaata ccggctgcaa
      781 ctgcgagctg gtgtagtaat caccggcgac tccgaacggg tcatgcttgc tgcggtagta
      841 gccgtgcttc gggtgataca gagcgagcga catgaagcgc tcgaatgaca tcggcccccg
      901 agcggcgatt tcggagcgaa tttcgcgttc cagttccgtc acttctcgtt ccggctttgc
      961 ggcgtacgag tgaacatctc tacgacatag tcgttcatac cgtcgtagag cagccgctga
     1021 aaggtccaag gaaactgcgt gctttccaag tctcgcggat gcagctccag gtaataggta
     1081 tgcaggccca aattcttcac cttgcagtcg attaccagtt cgatgcggcc ggcgcgttcc
     1141 cgcgcagaaa aatcgaaaag cggatgctcg tagcgtgcga tctgctgccg gacctgctca
     1201 agcagactca tccccgatcc agaatacaat aggaacgggc ggctgcgaac cccgcccggg
     1261 gcggccattg cgccggtatc acgccccgcc gcggccgcgt tggaagcgcg gcgggagcgt
     1321 ccggatggga acccggagga ggccagcggc tggcgtgact acatcctttc gcatgaggcg
     1381 cgaagcattc gaacaggccg tacgggaagc ctggcttgcc attcccgcca agttccgcca
     1441 gcgggttcac aacctggcca tcgtcgtaga agatgagcca agtcccgagc ttctgcgcca
     1501 gaatggcctt ccgccgggtt acgctctgct cggcctctat cacggcgtgc cgctcatcca
     1561 ccgcggcgcg agctacggca tggtgcttcc cgacaagatt tctctttttc aaggtcccat
     1621 tgaacgggcc gcgcaacggg cggacgacat tcgggaggtc atctacgaca cgctttggca
     1681 tgaaatcgct cactatttcg gcatgaacga ggcccaggtg cgggccgccg agcgcaagcg
     1741 cgcttcgcgg cgccggtttc ggcgctaggc tgtgtccggc cgctttctat gcccgccggg
     1801 aaacccgtgc taatctctcc taagaaattc tgccgccgca ggactggagg accgcaatgc
     1861 aacgcagaaa gttcgtacag ttggtgggta tgggggccgc gggagcagcc tcggcagtcc
     1921 cggccgacgc ggccgcccgc gcgaaaaaag gcgcatcgcc gcggcgcgct ctcatgaagg
     1981 ttggcacgca gcacggggat tccgatgaaa tccttcgcgt gctggcggcg ctcggagtga
     2041 ataatatttg cagcgccctg ccctcgcccc gcttcgatga aaagtggtcg gccgaaggcc
     2101 tgacacggct tcgggagcgg gtcgaatcct tcggcatcaa gctcgacatg gtcccgctgc
     2161 cgctcagctc cagttacatc acccggtccg aaaatcccaa catcatgctt ggcaagagtc
     2221 ccgaacgcga ccgcgagatc gataatatct gccagatgat tcggaatgcg aagcaggcag
     2281 gtattcccgc tttgaagtac aacatgtcga ttctcggcgt cgtacgcact gagcgcaccc
     2341 cgggacgcgg cggagcgagc tatagcacgt ttgtgtacga caaggccaaa caggagccgc
     2401 cgctcacgga agccggtccg gtcagcgatg agcaagcctg ggagcgcatt acgtatttcc
     2461 tgaagcgtgt gatcccggtg gccgaggaat acaaaatccg catggcctgc catccgcacg
     2521 atcccggaat gccgcgcgac aggggctttc gcggcgtcca ccgcgtgctc gggagcgtcg
     2581 aggggctgaa gcgctttatc gagatcaacc ccagcccgta tcacggcctc aatttttgcc
     2641 agggcacggt gtcggaaatg ctcgaaaatc ccggcaaaga gatttacgac gtgatccgct
     2701 atttcggcag ccgcaagaaa attttcaatg tccacttccg caacatccgc ggaaaatttc
     2761 tcaatttcca ggagacgttt atcgacgacg gcgacgtcga catgctgcaa gccatgcgcg
     2821 tgtacaagga ggtcggctac gacggtatga tgatgcccga tcacgtcccg cagatccagg
     2881 gcgacgcgca cggctaccag gcgtttgcct atacgttcgg ctacatcaaa gcgctgatcc
     2941 gggctgtgga cgcggagggg tgagccggct cgaagagtcg cctacggcag ccggcttctt
     3001 ttcggcagtg ttcctatttg ggttccagca ccagtctgcg ggccgtgttc ttttcgacgg
     3061 cggcacgaga atacggcagc ggaaaatact ccccgtcggc ccacagaggc aacaggtcgg
     3121 catagtgcgg gctgcccggc tggccggatt gccccggagc gttgatcgcc accgagcggt
     3181 cccagtcgga aaggtcgaaa atctcgcgat aggatgcgcc tgaagcctgc acaaaccccg
     3241 gccctcccgt t
//
LOCUS       DASREK010000189         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_425705, whole genome shotgun sequence.
ACCESSION   DASREK010000189 DASREK010000000
VERSION     DASREK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425705"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFA54_09795"
     CDS             complement(<1..677)
                     /locus_tag="VFA54_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HZS51142.1"
                     /translation="MNDERLRRLRDLLAEARVEGLVVSALPNIRYLTGFTGSNALLLV
                     TGEDALFLTDGRYEIQAAREVQGARVRVPGGSLMRALIASVKRRRLKSLGFEAKRASF
                     ELYQALHTGLKACRLRPLAGLPERLRAIKSAEEITAIRRSVHLNSAAFEKSIPEIRNG
                     RTEAELAAIIEYEMHALGGEKPAFETIVASGLRSALPHARPTDKPLQKKQLIIVDQGV
                     ILDGYSS"
     gene            complement(674..1066)
                     /locus_tag="VFA54_09800"
     CDS             complement(674..1066)
                     /locus_tag="VFA54_09800"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HZS51143.1"
                     /translation="MRAFSSAPSGERSSEAAGQPGGEFHLNQVSLIRERDGLTVALGI
                     EPGAKYQTMTLAHPNRVVIDFLNCRLTVPPPYSRPLDTPPVKRLRISQFQLEPPITRL
                     VLDADTIPQYRIQPSSTGIEIHMTGGSL"
     gene            complement(1467..2450)
                     /gene="pilM"
                     /locus_tag="VFA54_09805"
     CDS             complement(1467..2450)
                     /gene="pilM"
                     /locus_tag="VFA54_09805"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZS51144.1"
                     /translation="MFPFSNLRPFGLDIGSATLKAVELERKNGTITAPRAAIASMPGG
                     AFSGGVLLDPPAAAEAIRKLCREHRFRGKHVITGVSGSDVFVARVKLERGAPETLAAR
                     IRERACDAAPFSLEQAYLDYQLIDNFSDSQWVDVLVAAVKPGRLDVLQDVLRRAGKRA
                     TIVDVVSCALANVFEVNYAPAPSAVSGLLHVGASTLTVCIVRGSTPLIAEEVSLTTVA
                     GETAELNRAERVAIELERIFEQMDEIADEHPLEPGSGQIERLFLSGGGARSRGLPEVL
                     KERIGLPFEELNPFRKIELSGSDTLSRLVWDHAHCMPVAVGLALRGLDERG"
     gene            complement(2489..3136)
                     /locus_tag="VFA54_09810"
     CDS             complement(2489..3136)
                     /locus_tag="VFA54_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51145.1"
                     /translation="MIGRSSLVIQETGAVLVTVLLLSSGCIFQHRTPQAAAPPVAAPA
                     PGPLSSPQITETLPPPQPIPAGAVPPEIGAPVAASLPSPEIPPAPPPRPVRRNVRPTT
                     VPPPAAAAVAPPARNEGNPSVATAASPQLRPMLTAAQERQLQQSINRSLANAEQLLAR
                     LGNVQSDRERAASVALVRSFIEQAQQARRKGDLNGARSLAERAELMAADLSQGAK"
     gene            complement(3133..>4517)
                     /locus_tag="VFA54_09815"
     CDS             complement(3133..>4517)
                     /locus_tag="VFA54_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS51146.1"
                     /translation="VLVTHVAVSTTFLRQQLGPQIRQLEYAALISLLAALALSVAFSR
                     IAFRPLDRLGEAIDRMTRGEFAGALPADVPKGSEYAVIRSKLNLLGQQFRDAREGISS
                     LRGNMEQLMRQLEGAVLLFDANDRLIIASAAAEEFLGLGRWQMMGLTIDEIFPAGTQL
                     GALVGSATRLRQPIQNQTVELEPGPNGQVGANGTARPSRLLLTVELMEDFANHRRLGT
                     LVLLRDAETRREIQTQVEISNRLAAISRLTSGVAHEIKNPLNAITLHLELLKNRLSGV
                     LPETPQELNVILREMARLDHVVKTFLDFTRPVNLRLTEVELDKLLEEIANLARPEAAQ
                     HQVRIQILNGCNGTVIKGDRELLVQALLNLAVNGIQAMPQGGDLTMALARAGPEVELT
                     VTDQGVGIRSEDKEKIFRLYFTTKKTGSGIGLAMTFRVVQLHNGTIDFSSEVGRGTTF
                     RVRFPLVEAR"
BASE COUNT          850 a         1402 c         1403 g          862 t
ORIGIN      
        1 tcgcttgaat atccatcgag tataacacct tgatctacaa tgataagttg ttttttctgc
       61 aagggtttat cggttggccg ggcgtggggc agagccgatc gcagtcccga tgccacgatc
      121 gtctcgaagg ccggcttctc gccgcccagt gcatgcatct cgtattcgat gatggcggcc
      181 agttccgctt ccgttcgtcc attgcggatt tcagggattg atttttcgaa ggccgctgag
      241 ttcagatgaa ccgatcggcg aatcgcagtg atctcttcgg cgctcttgat cgcccggagc
      301 ctctccggaa gcccggccag cggccgcaaa cggcacgcct ttaggccggt atgcaatgcc
      361 tgatacagct cgaagctcgc ccgcttggct tcgaagccca acgatttcag ccggcgcctt
      421 ttgacggagg cgatcagggc gcgcatcaag gatccgcccg gcacccgcac cctggcccct
      481 tggacttccc gggccgcctg aatctcatac cggccgtcgg tcagaaagag tgcatcctcg
      541 ccagtgacga gcagcagcgc gttgctgcct gtaaatcccg tcaggtagcg aatattcggc
      601 agagccgaaa cgacaagacc ctccacccgg gcctccgcca acaggtctcg cagccggcgc
      661 agccgctcat cattcatagg ctgccgcctg tcatgtggat ttcaattcct gtcgagctcg
      721 gttgtatgcg gtactgaggg atagtatcgg cgtcgagaac cagccgggtg attggcggct
      781 cgagctggaa ctggctgatg cgcaatcgct tcacgggcgg cgtatcgagg ggccgcgagt
      841 agggtggagg aacggtcagg cggcaattta gaaaatcgat gaccacccgg ttcggatggg
      901 ccagcgtcat ggtttggtat ttggcgccag gctcgattcc cagagccacg gtcaagccgt
      961 cgcgctcgcg gatgagagag acctgattca ggtgaaattc tccgcccggc tgtcccgccg
     1021 cttcgctcga tctctctccg gagggggccg aagagaatgc ccgcaaagaa tggccatcca
     1081 aagacgccgg tggagcgctc gcggcttctg aagcgccaga ggaggaaggg gctggagccg
     1141 gcgaaggcgc gccgggcgct ttggattggt tttccatctc tgcgccgcgg gcggggatcg
     1201 gactttgggc ggccgcttcg cttttggcga agctcttccc cgcatccttc ggttccccgc
     1261 tttgcactgg cgccggaacg cgcactgttc gccgttccgc caaatagaat aaaattgcca
     1321 ccgcgctggc aagcagcagg ataccggtcc acgcctcacg gcgggtgatc caggcgctgc
     1381 cgcctgagtg atcaagcgcc tctctcggac tttgccggct gctttgcctc agtaaattga
     1441 ttcggatcat gaaggcgcgt gaattctcat cctctctcgt ccagtccgcg caaggccagg
     1501 ccaactgcca cgggcatgca gtgcgcatgg tcccaaacca ggcggctaag agtgtctgag
     1561 ccggacaatt cgattttacg gaaaggattt agctcctcga acggcaggcc aatgcgttcc
     1621 ttcagcacct cgggaagacc gcggctgcgg gcgccacccc cggaaaggaa cagccgctcg
     1681 atctggcctg atcccggctc gagcggatgc tcatcggcga tttcatccat ttgctcgaag
     1741 attcgctcca gctcgatggc gacccgttcg gcgcgattga gctccgcagt ttcccctgcg
     1801 acggtcgtaa gagaaacctc ttccgcgatc aacggcgtgg agccgcgtac gatgcaaacg
     1861 gtcaaagtgg acgcccccac atgaagcaat cccgagaccg ccgaaggagc gggcgcataa
     1921 ttcacctcga agacattggc cagggcgcag gagacaacgt caacaatcgt cgcgcgcttt
     1981 cccgcccggc gcaatacatc ctgcaaaacg tccaatcgcc cgggcttcac ggcagctacg
     2041 agcacatcga cccactggga atcggaaaaa ttatcaatca attgataatc cagatatgcc
     2101 tgttcgagag aaaacggcgc ggcgtcgcag gccctttccc ggatccgcgc cgccagcgtc
     2161 tcgggcgcgc cgcgctccag cttgacccgg gcgacaaaca catcactgcc gctcacaccc
     2221 gtgatgacgt gcttgcctcg aaaccggtgc tcgcggcaaa gcttgcggat cgcctcggca
     2281 gccgcgggag gatccagcaa caccccgccg gaaaaggcgc ctccgggcat ggaggcgatc
     2341 gccgcgcggg gagcggtgat cgttccattt tttctctcga gttccaccgc ctttagcgtg
     2401 gcggagccga tatcgaggcc aaagggacgg aggttgctga agggaaacac agtttcggat
     2461 ttcctcagcc gcgcatttcc aggatgggct atttggcgcc ctgcgatagg tcagccgcca
     2521 tcaattcggc gcgctcggcc agactgcggg ccccattcaa atcgcctttg cggcgggcct
     2581 gttgcgcctg ttcaataaac gagcggacca aagccaccga ggcggcgcgc tccctgtccg
     2641 attgtacgtt gcctaagcgc gccaaaagct gttcggcgtt agccaggctc cggttgatgc
     2701 tctgctgcaa ttgccgctct tgcgcggcgg tgagcatcgg acggagctgc ggagaggcgg
     2761 ccgtggcaac cgagggattt ccctcgttgc gggcgggtgg cgcaactgct gctgccgccg
     2821 gcggcggtac agtggtggga cgcacattgc ggcggacagg tcgtggcggc ggggccgggg
     2881 gaatctccgg cgaggggaga gaagccgcca ccggcgctcc gatctccggc gggaccgcgc
     2941 ctgccggaat cggttgggga ggaggcagcg tctctgtaat ctgcggcgaa gagagaggcc
     3001 ccggcgccgg agccgccacc ggaggtgcgg ccgcctgcgg agtgcggtgc tggaaaatgc
     3061 agccggacga gagaagaagc accgtcacca aaaccgcgcc ggtttcctgg ataaccaagc
     3121 tgctacggcc gatcatctgg cctccactaa aggaaacctg actcggaaag ttgtacctcg
     3181 gcccacttcg ctggaaaagt cgattgtacc attgtggagc tgcaccacgc ggaaggtcat
     3241 agccagccca attccggacc ccgtcttctt ggtcgtgaag tagagacgaa agatcttctc
     3301 tttatcttct gagcggatgc caacgccctg atccgtcacg gttagctcca cctccggtcc
     3361 cgcccgcgcc agcgccatcg tgagatcgcc gccctgaggc atagcctgaa tcccattcac
     3421 cgcgagattc agcagagcct ggacaagcaa ctcccggtcg cccttgatga cagtgccatt
     3481 acagccgttg aggatctgaa tgcgcacttg atgctgggcg gcttcgggcc gtgccaagtt
     3541 cgcgatctcc tcgagcagct tgtccagttc gacttcggtg agccgcagat tgacaggccg
     3601 ggtaaagtcg agaaaagttt tgaccacgtg atcgagccgc gccatctcgc gcaggatcac
     3661 attcaattcc tgaggcgttt ccggcagcac cccgctcaaa cggtttttca gcagctcaag
     3721 atgcagggtg atggcattca gtggattttt gatttcatgc gccacgccgc tagtaagcct
     3781 gctgatggcc gccaggcggt tggaaatttc cacttgggtc tggatctccc ggcgggtctc
     3841 ggcgtcgcgg agcagaacga gagtgcccag gcggcggtgg tttgcgaagt cctccatgag
     3901 ttccactgtg agcagcaggc gggagggccg tgcggtcccg ttggcgccca cctggccgtt
     3961 cgggccgggc tccagttcca ctgtttggtt ctgtatcggc tgccgcaggc gggtggcgct
     4021 gcccaccaag gcccccaact gagtgcccgc cggaaagatt tcgtcgatcg tcagccccat
     4081 catctgccac cgccccaggc ccaaaaactc ctcggcggcg gcgctggcga tgatcagccg
     4141 gtcgtttgcg tcgaaaagca gcaccgctcc ctcaagctgg cgcatcaact gttccatgtt
     4201 gccgcgcagc gaagagatgc cttcccgtgc gtcgcggaac tgttgcccga gcaggttgag
     4261 ttttgaccgg ataaccgcgt attcgctgcc cttgggaacg tctgccggca gcgcgccggc
     4321 gaattcgccg cgcgtcatgc ggtcgatcgc ctctccgagc cggtcgaggg gccgaaatgc
     4381 gatcctggaa aacgccacgg agagggccag ggccgccagc aggcttatca gcgccgcgta
     4441 ctcgagttgg cgaatttggg gcccgagctg ttgccgcaga aatgtcgtgg aaacggcgac
     4501 atgcgtcacc agcaccg
//
LOCUS       DASREK010000190        15038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_762814, whole genome shotgun sequence.
ACCESSION   DASREK010000190 DASREK010000000
VERSION     DASREK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 15038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15038
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762814"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VFA54_09820"
     CDS             <1..753
                     /locus_tag="VFA54_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZS51147.1"
                     /translation="QSDTAVSASAVTLFAVCPRKYYLSRYLGFEESRMSSQPLPKDDT
                     SDSERDETDPSEFGRQVHALLAGEDAPGAGPEALRLVKNFEGSEWGRRLLRATRIERE
                     QEFLILVGPPAEQRLLRGQMDLWFEDAGEIVLLDYKTDQASAAEVAGKAAEYALQLRL
                     YALALEQIARKRPDRAVLYFLRPNTAAEIPVDDASLEAARAKVEELYQAQSSQIFPLH
                     VGKHCFQCPHYRGACPAQHPEALAASQATQSK"
     gene            complement(961..4260)
                     /locus_tag="VFA54_09825"
     CDS             complement(961..4260)
                     /locus_tag="VFA54_09825"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS51148.1"
                     /translation="MVHPGRLKYGALLLALAVFACVSPSSLSAQTLKGTILGTITDAS
                     HAVIPGVQVNITETNTNFHRVETTNESGFYAFANLDPGNYRVEVEQPGFRKVVRSDIE
                     LAANTTVRVDLELQPGQVTEVVDVTAEAPVLQTDRADTGGKIESQQLANLPLLNNRNY
                     QNTLMLVPGVQRSYRSNSPFFNSQEHLQSVVNGLDEKNNYMIEGIDNNIEGYMTAVVL
                     PADAIATVDVSTTNYDPELGRAGGAVVNVIMKSGTNDFHGSLFEYHRDSDLQARNVFA
                     TTVPHGVYNQFGGSGGGHIIRDKLFFFGDYQGSREIVGQNAIPTIPTMPMRGGDFSAS
                     PTTIYDPNTGDSSGRGRTPFPNNQIPANRISPIAAQYLSFLPPPTTPGLSNNYQGPNS
                     QNKAIDQFDTKIDYVIGANDRMFFRYSFQRAFVTNPGLYGPGLGIYGGPSNNGFDATG
                     PSRNQSPGLNYSHIFSPTLVMEARFGIVRNRNDATNVDTGLPLSQKLGIPNGNLGDFW
                     TSGLAEVFVNGYDTPMIGINGCLPWRRAVTNFDFVNNWTKTKGTHVVKWGFDLRRERR
                     DLLQTATFSPRGRFTFTPGPTALNGGPASGQFNAFAAFLLDQPNSVGRDLAVVFPTQR
                     DWVYNLYFQDKWQVTRKLTLDLGLRWEYWPAGTSAFPGGASNYIPANNTLQLNGLGNI
                     PMDSGIDNHPLGFGPRFGVAYRLDEKTVLRGGFGISQFYRYTTSSLWQYPVKQNQQLV
                     APNSFVAAGSMATGFPAPIPAVIPSNGIITNPPNQTFTALPKNVPVPYVESWNIAVQR
                     SLPANLALDVAFVGNHGVHIGPPTGNSININASRIPGSGTAGEPLNILFGRTAATNVP
                     DFESTFYDALQIKLNRRFSNGFTMTTSYAFGKSIDYLAGSSGLNNLDIKRNKGLADWD
                     RRHIFTYSGTYDLPFGQGKKWATSGPAKWALGGWQVTGLWTWESGLPLDIITSSTSLN
                     APGLTNRPNQVAPVKILGNVGPGQYWFTASSFANPPALTFGNVGRNILNGPRLFNIDF
                     SLLRRFRITERVHMEFTASAFNLSNTPWFDRPDTNLQDAAFGQVTTAQGNQAVKVNMN
                     RSMQGSLRLTF"
     gene            5098..6231
                     /locus_tag="VFA54_09830"
     CDS             5098..6231
                     /locus_tag="VFA54_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZS51149.1"
                     /translation="MLPRLFFASLCLELMAFLVFAQAPEPVRSPEVLPDHRVTFRFRA
                     PNAKEVAVAREGLPQPVPMQKDERGVWSVTTDPLEPDFYGYSFLADGVRLVDPSNHLI
                     KPNLLTLTNMVHVPGPGSLPWEVNDVPHGAVQERWYKSAVVGDTRDFFVYTPPGYDPQ
                     AKKQYPVLYLLHGFSDDASAWVSVGRANVILDNLIAQGKAKPMIVVMPLGYGAPEIVE
                     PGGPGFRDPDARQRNLEKFRDALLTEVIPQVEKQYQVTRDRNARAIAGLSMGGAESLY
                     VGLNALDRFAWVGSFSAGGLGENFDAVFPLLSPKANSRLRLLWVACGTEDRLIEPNRK
                     FREWLKSKEIQHTSIETPGMHTWMVWRRNLANFAPLLFQKSAT"
     gene            complement(6277..6552)
                     /locus_tag="VFA54_09835"
     CDS             complement(6277..6552)
                     /locus_tag="VFA54_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51150.1"
                     /translation="MMRALQTGRPAPKPGLWVHVGGARVIDLRPNRGDKGAQEALRHS
                     LEMGTAAEQDPKRVSFYETSLDGYRYYFHVAPSRPPRVYLLARWPEP"
     gene            complement(6549..7742)
                     /locus_tag="VFA54_09840"
     CDS             complement(6549..7742)
                     /locus_tag="VFA54_09840"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS51151.1"
                     /translation="MNKKKKSGWWGIAAALVLIGGGVVLFTALSKTSVKLDPTKLARV
                     QRGDLARSVVATGKIEPIARVEIKSKASGIIERLLVNVGDTVKEGQVLAELDRDQLNA
                     RVRQLEATLAASEANVVATRASYEKMLSDAKGIDVPFLKRQFERSQGLYKEGLIPAQA
                     LDDAEKAYQVAVNRQEQAIASANSARAEIGQAEARVKESRAALEQAREDLQYATIRSP
                     LNGIVLSRNVEIGDAVSSILVMGSAATLVMTLGDMRELYLKGKVDESDIGKVYLGQPA
                     RITVESFKERKYEGRVTRISPMGEEKDNVTTFEVRISILNPRDLKALMTANGEIILEE
                     HKNVLMLPEAAVLYDRDKSTWVEVPDASQKTGKRKIPIQTGISNGANTEVLAGLREGQ
                     EVVLQ"
     gene            7931..9307
                     /locus_tag="VFA54_09845"
     CDS             7931..9307
                     /locus_tag="VFA54_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZS51152.1"
                     /translation="MEDFPVVFRLILLPLLLGINGFFAAAEVALISVRLTRIAQLAEK
                     GNPRARAVVDLLANPDRLLSATQLGVTLASLGLGWAGEDTVYRLILAVIPSSWPAVST
                     ALSHPQWHRLLHVAAFVIAFLAITFLHMVVGEVVPKNLAFERAERLALAVGPTLEFFA
                     GVTNFFIRIVERSSESLSRLLGVRGKPRPAAHTAEELRLIVTASRRHGELPRSQEEML
                     HNVIDFYDLTVRQVMVPRNEIVSVSADATFDQVVRTIVEHQHSRLPVYERTPENIIGI
                     LYAKDLWMFQPRGRGFRLRAIIHPHKVVPETKPLHQLLAEFQQERFHMAMVVDEFGTI
                     VGLVTVEDLLEQIVGEIRDEHDLPEPARAPASGPIELDGATNVRDLENLYSIHVPYDA
                     GFQTLAGFLLEKLGSIPRGGEQVEHEGRRYTVVEMEGRRIARVRIEEISEEPAGEEAR
                     PREPLAER"
     gene            9356..9958
                     /locus_tag="VFA54_09850"
     CDS             9356..9958
                     /locus_tag="VFA54_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51153.1"
                     /translation="MVTFDLANYPKRIAEVLAKAGPEERPGERLMPLAFGPCVSPASR
                     DLLRSLGTADLFAPSQVAADDYAAAARSGLFVYLSCMDDAHAIAQEIHTATGSYWHGI
                     LHRMEPDFSNARYWFRRVGRHEIFPALRAAAAALAGEDIPEFRNAAQWDPFAFIDLCE
                     QEGGSGSGKRAKALERVQLAEWQLLFDFCCRRALGIPWGK"
     gene            9963..11627
                     /locus_tag="VFA54_09855"
     CDS             9963..11627
                     /locus_tag="VFA54_09855"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZS51154.1"
                     /translation="MSARHLCLLALLLLSGCSHQQDTPYDLIISGGSVLDGDGGEAIR
                     ADIGVRDGKIAKLGDLSQAFAHRRIRAEGLVVAPGFIDIHNHSDYTILQEPKAESMIR
                     QGVTTMVLGEGESAGPVRPGSAQTRIEGVNVDWTTLGGYFARLERQHMATNIASYVGE
                     GQVWACVKGETQSPAAPAELEQMKRLIAQAMQEGAMGLSTSLLMPPSSLATTGNLIEL
                     ARMAKQYGGIYSSHIRDEGEGVFRSVAEAIAVGRGAQIRVDVIHLKIAHKNLWGRMNE
                     VAGMIQKARDEGLDIWANVYPYTAGQNNLSAIIPPWAHDGGNEKMLERLRDPKLRERM
                     RREILTGLPGWYNHYLATGGGWAGMLLVSLKADRNKPFQGKRMSELIAARGGDPVNVL
                     FDVLLEENGSVPTVYFHHSEPDMELALKQPWTSIGSDGAAVSPYGPTSHTHPHPRYYG
                     AFPRVLGRYVRELHVLSLPEAVKKMTSMNADKLGIRDRGRIKEGNWADITIFDPEKVA
                     DQATYENPHQYPLGISYVIVNGAVTIDNGNHTGALAGKVIYGPGRK"
     gene            11608..13158
                     /locus_tag="VFA54_09860"
     CDS             11608..13158
                     /locus_tag="VFA54_09860"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1736 domain-containing protein"
                     /protein_id="HZS51155.1"
                     /translation="MGRAANKVKPACEPRPARLGRPVFAAGLIFLAACAVYGNSLGGR
                     FVFDDTSIIESNPQIRSLGWASLERIFGSHYWQAVQGQGGLYRPVVVFSYALNYAAGA
                     LDPWGYHLVNVLLHGLNAALVFLIFQELFEDRIFAVWSGLLFALHPIHTEAVAYVVGR
                     AETLAALFFLIAWLCYLRRRIGAAAAAFFLAVLTKESAFTFLAVPPLSDYVRERRLTP
                     LRYAPFAGAAGLALALRYAVLGGVAPLSINPTSNPLAAAGTAARLLTATCVFGKYLWL
                     LVFPVPLSADYSFNQIPPVTTPWNLWFAVSAAALALMAGGIVFAFRRSPPLFLCGAFF
                     CAAFSLTSNFIRPIGTIMAERLLYLPSLGFTGALGYLIARGWRSPRRRDMATAASVLL
                     AVFYAGRTVLRNADWKDHLALFSSAAVVSPNSSLVQANLASALLYQKGDAAGAAEHAR
                     EAIRIEPADPAAHMTLGEACQRLGDLRQAIEAYQAVERLAPGTAGAHEAARRKQAILR
                     SLGHNAPQ"
     gene            13367..14890
                     /locus_tag="VFA54_09865"
     CDS             13367..14890
                     /locus_tag="VFA54_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HZS51156.1"
                     /translation="MRKACVNLLLGALVTAGLGSAQTPSVVFSVKAYGAKGDGKTLDT
                     AAVNKAIEAASSAGGGTVYFPPGTYVSGSIHLRSRVTLFLGQGSVLEASPDPEVYDPI
                     EESQKQWAKFQDFGHTYFHNSLIWGEGLEDIAIVGPGLIYGKGLARAADQRVGNKAIS
                     LKLCRNVTIRDVSMLMCGHFAILATGVDNLTIDNIKIDTNRDGIDVDSCRHVRISNCS
                     VNSPRDDAIVIKGSHALGFARATEDLTITNCEVTGFEEGSFLNGTYKRRGPTPERNGP
                     TGRIKIGTESEGSFRNITISNCVFDYSRGLALETVDGAQLEDISITNITMRDIANAPL
                     YLRLGRRMRAPEGTPIGALRRVNISNLVVYNADPRYGSMIIGIPGRDIQDVSLSNIRI
                     YYQGGGTKEQAAINPPENETEYPEPYRHGVMPAYGFFIRHAQGVQMTNVEVSYLKEDL
                     RPAFVLEDIKGAEFNLVKARHAPGVPTFRLKGVEDFSLHRCGALPDMRLPKAQQKEF"
BASE COUNT         2867 a         4453 c         4614 g         3104 t
ORIGIN      
        1 cagagtgaca ctgccgtttc ggccagcgca gtgacgctgt tcgcagtctg tccccggaag
       61 tattatctgt cgcggtatct gggctttgaa gaaagcagga tgagctccca gccgcttccg
      121 aaggatgata cttccgatag cgaaagagac gagaccgacc cgagcgagtt tggccggcag
      181 gtacatgctc tgctggccgg agaggatgcg cccggggcgg ggcccgaagc cctaaggctg
      241 gtcaaaaatt tcgaaggcag cgagtggggc cggcgtctgc ttcgagcaac acgcatcgag
      301 cgggaacagg agtttttgat tctggtggga cctccggccg agcagcgtct gctgcgcggc
      361 caaatggact tgtggtttga ggatgccgga gagatcgtgc tgctcgacta taagacggac
      421 caggcgagcg cggcggaagt cgccggaaaa gcggccgagt acgcgctgca actacgtttg
      481 tacgctcttg ccctggagca aattgcgcgc aagcggccag accgggccgt tctttacttt
      541 cttcgcccga atacggcggc cgaaatcccc gtagatgacg catcgctcga agccgctcga
      601 gccaaagttg aggaacttta tcaggcgcag tcgagccaga tcttcccact acatgtgggc
      661 aaacactgct ttcagtgtcc ccattaccgc ggcgcttgcc ccgcgcagca tccggaagcg
      721 ctggctgcgt cgcaagctac ccaatcgaaa taggtcggta agcgccggcg ccgggcaagc
      781 tgcgcagact actccagcgc cccggatctg tagctttgaa gccgcctctc ggaatttcag
      841 aaacgcggga aacgagacgc ccgaagcggc tgaaaagtcg cttcgggcag atcgatccct
      901 gagaatgaag agctccggca gcagcggcca ttgtgcgccg ctgctgcgaa gcttccgagg
      961 tcagaaagtc agccgcaagc tgccttgcat cgaacgattc atgtttactt tgaccgcctg
     1021 atttccctgt gccgtcgtta cctgaccgaa cgcagcatcc tgaaggttgg tatccggccg
     1081 gtcgaaccac ggcgtgttgc tcaggttgaa cgcggaagcg gtgaactcca tgtgcacgcg
     1141 ctccgtgatc cggaatcgcc ggagcaacga gaagtcgatg ttgaacagac gcggaccgtt
     1201 gagaatattc cgccccacgt tgccgaaggt cagggccggg ggattggcga acgaggaagc
     1261 cgtaaaccag tactgcccgg gtccgacgtt gccgagaatt tttaccggtg cgacttggtt
     1321 cggacgattc gtcaggcctg gagcattcag gctggtagag ctggtaatga tgtcgagagg
     1381 cagcccgctt tcccaggtcc acaggccggt aacttgccag cctcccagcg cccacttggc
     1441 cggaccgctc gtcgcccact tcttaccctg gccgaaaggc aggtcgtagg tgccgctgta
     1501 ggtaaagatg tgcctgcggt cccagtcagc aagccctttg tttcgtttga tatccaggtt
     1561 attcaggccg ctgctgcccg cgaggtagtc gatcgatttc ccaaaggcgt acgacgtagt
     1621 catcgtgaag ccgttggaga aacggcgatt gagcttaatc tgcaacgcat cgtaaaaggt
     1681 cgattcgaaa tctggcacgt tggtggcggc ggtccgtccg aacaaaatat tcagcggctc
     1741 gcccgccgtt ccgctgcccg ggatccggct ggcgttaatg ttgatcgagt tgcccgtggg
     1801 gggccctatg tgcacgccgt gattgcccac gaaggccacg tccaaagcga gattggccgg
     1861 aagcgagcgt tgcactgcaa tattccagga ctcaacgtac ggcacgggaa cgtttttcgg
     1921 caaagccgtg aaggtctggt taggcggatt ggtgatgatc ccgttggagg gaatcacggc
     1981 tggaatcggc gcaggaaatc cggtggccat tgagccggcg gcgacgaaag agttcggcgc
     2041 cacgagctgc tgattctgct ttaccggata ttgccacaac gaactggtag tgtagcgata
     2101 gaactggctg atgccaaacc cgccgcggag gaccgtcttc tcatccagcc gataggccac
     2161 gccaaaccga gggccgaagc ctaaggggtg gttgtcgatg cccgaatcca tggggatgtt
     2221 ccccagccca ttgagctgga gcgtgttgtt ggccggaatg taattggaag ccccgccagg
     2281 gaatgccgat gtgcccgccg gccagtattc ccagcgcagg ccaaggtcca aagtcagttt
     2341 gcgcgtcacc tgccacttgt cttggaaata aaggttgtag acccagtccc gctgggtggg
     2401 gaagacaacc gccaggtcgc gaccaacgct gttcggctgg tccagcagga aggcggcgaa
     2461 agcgttgaac tggccgctgg cgggaccgcc gttcaatgcc gtcgggccag gcgtgaacgt
     2521 gaaccggccg cgggggctga atgtagcagt ttgcagcagg tcgcggcgct cgcggcgcaa
     2581 atcaaacccc cacttgacca cgtgcgtgcc cttggtcttc gtccagttgt tcacaaagtc
     2641 gaagttggtg acagcgcgcc gccacggaag acagccgttg atgccgatca tcggcgtgtc
     2701 gtagccgtta acgaaaacct ctgcgaggcc gctcgtccag aaatcgccca ggttgccatt
     2761 gggaattccg agcttctgcg acagcggcag gcccgtgtca acgttggtcg catcgttgcg
     2821 attccgcacg atgccgaagc gcgcctccat gaccaatgtc gggctgaaga tgtgcgaata
     2881 gttgagtccg ggactttggt tgcgcgaggg cccggtagcg tcaaagccgt tgttcgaggg
     2941 gccgccatag atgcccagac cggggccata taacccggga ttggttacga atgcccgctg
     3001 gaaactatac cggaagaaca tccggtcgtt ggctccgatc acatagtcga ttttcgtatc
     3061 gaactgatcg attgccttat tttgcgaatt gggtccctgg taattgttcg acagcccggg
     3121 agtggtgggc ggcggcagga agctcaggta ttgcgcggca atcggactga tccggttggc
     3181 cggaatctga ttattcggga aaggtgtgcg ccctctgccg ctcgaatcgc cggtattcgg
     3241 atcgtagatg gtggtcggcg atgcgctgaa gtcgcccccg cgcatgggca tcgtcggaat
     3301 cgtgggaatc gcgttttgac cgacgatctc ccggctgccc tgataatcgc cgaagaagaa
     3361 caacttgtcg cggatgatat gcccgccgcc gctgccgccg aactggttgt aaacgccgtg
     3421 cggcacggtg gtggcgaaaa cattcctggc ctgcaagtcg ctgtcccgat gatattcgaa
     3481 caagctgcca tgaaaatcgt tggtaccgga tttcatgatc acattaacca ccgcgccgcc
     3541 ggcccggccc aactcaggat cgtagttcgt cgtgctgacg tctaccgtgg ctatcgcatc
     3601 ggcgggcaag acaacggccg tcatgtatcc ctcgatattg ttgtcgatgc cctcgatcat
     3661 gtagttgttc ttctcatcaa gcccgtttac gactgactgg aggtgctcct gggagttgaa
     3721 aaacggggaa ttcgagcggt agctcctctg gaccccgggt accagcatga gcgtgttctg
     3781 ataattgcgg ttgttgagca acggcaggtt agccagttgc tggctctcga ttttgccgcc
     3841 cgtatccgcc cgatcggtct gtaggaccgg agcttcggca gtcacatcga ccacttcggt
     3901 cacctgcccc ggctggagct ccaagtccac acgcaccgtt gtgttggcgg ccaactcgat
     3961 atcggaccgg accaccttgc gaaaaccggg ttgctcgacc tcgacccggt aattccctgg
     4021 atccagattc gcgaaggcgt agaatccgct ctcattagtt gtttccactc gatgaaagtt
     4081 tgtgttggtt tccgtgatgt taacctgcac tcctgggatc accgcatgcg aggcgtcagt
     4141 aatagttccg agaatcgttc ccttcaaggt ctgcgctgaa agtgacgaag gcgatacaca
     4201 tgcgaagacg gccaacgcca gcagtaaagc cccatacttc agtcgtcctg gatggaccat
     4261 cgtttcctcc catcgccggc taaggcgaca cactcccgat accgcacggc ttttcaggct
     4321 gtgctgcttt aggggcgccg gcgaaacaac ttcactggcg cttatctatc aagcctctaa
     4381 gaaaagcgaa ggacacgaat atccggcatc gccaccccgg cggcgccagt tcgaaagctg
     4441 agtccgttca ggcccactcc ccaaagcatc cttatcaaac gcgtgctgga ctcagtataa
     4501 gacgaaaaat cgaaattgca agggcagaat cccagaatca agcggtagta ctaaccggta
     4561 ggcaagtccc cctagaactt tcgattcggg ctgtgcctgg ggcgccacct aagagcaagt
     4621 gttggccgga gagcgcccgc agaaatctct gcgccggctt tcggaatgcg cgattttatt
     4681 aggaccggcg aacgcacaac ctcgatccga aaagtagatc cttggttggg ttccattgat
     4741 tggccacaca aactcagggc atggtcaagc tggagaagcg cgccatgcgc ttacggtctt
     4801 gacaatagct gaacaagtgt aaaatttttg tctgcccgga gggaaagccg gcggccgcgg
     4861 tcgccgttcc gctcgtgccc ggtctcttgg agcgacggct gcgcggccga tgttaagttc
     4921 cgtgttccgg tcttcgccgc acaatgctgg gtgctcagca gcgcctctgg gccggccgcg
     4981 cgggcaaaag acggaaaggc agggaacggt tatactcgct gtacggattc gccattcgtt
     5041 gccgcccgtg caccgctgcc cgtggctgca tggcggattg gcggagaaag gaggagtatg
     5101 ttgccacggc tgtttttcgc ttcgctgtgc ttggagctga tggccttctt ggtgttcgcc
     5161 caggcgcccg agccggttcg ttcgcccgag gtgctaccgg accatcgcgt cactttccgt
     5221 tttcgcgctc ccaacgcaaa agaagtggcg gttgcccgcg agggactccc acagccggtt
     5281 ccgatgcaga aggacgagcg gggcgtttgg agcgtcacaa cggaccctct ggaaccggat
     5341 ttctacggat actctttcct ggccgatgga gtgcgtctgg tagacccctc caatcacctg
     5401 atcaaaccga acctactaac gctcaccaat atggtgcacg tacccgggcc tggctcgctt
     5461 ccctgggagg tcaacgacgt ccctcacggg gccgtccagg agcgctggta caaatccgcc
     5521 gttgtcgggg atacacggga tttcttcgtc tacactccgc cgggatacga tccgcaggcg
     5581 aagaaacaat accctgtgct ctatcttctg catggtttca gcgacgatgc gagcgcctgg
     5641 gtttcggttg gccgggcgaa cgtcattctc gacaacctga ttgcccaagg gaaggccaag
     5701 cccatgatcg tcgttatgcc tctgggatac ggcgcgccgg agattgtaga gcccggggga
     5761 cccggcttcc gcgatcccga tgcccgccag agaaacctgg aaaaatttcg agatgccctg
     5821 ctgaccgaag tgattcctca ggtcgagaaa cagtatcagg tcacgcggga cagaaacgcc
     5881 cgggcgatcg ccggtctttc gatgggaggg gcggaatctc tttatgtcgg cctgaatgcc
     5941 ctggaccgct tcgcgtgggt gggctcattt agcgcaggcg gtctggggga gaactttgat
     6001 gcggtgttcc cattgctcag tccgaaggcc aattcgcggc ttcgcctgct ctgggttgcc
     6061 tgcggtactg aggaccgcct gatcgagccc aaccgcaagt ttcgcgagtg gctcaaatcc
     6121 aaggaaatcc agcacacgag catcgagacg cccgggatgc acacgtggat ggtgtggcgg
     6181 cgcaatctcg ccaatttcgc tccgcttctg ttccagaaga gcgcgacgta gagcctcttc
     6241 ccgccgtccc agagccggcc gcccggcggc gtatgcctac ggctccggcc agcgagccag
     6301 aagatagaca cggggagggc ggctcggcgc cacgtggaag tagtagcgat atccatccag
     6361 ggatgtctcg taaaagctga cccgcttggg atcctgttcc gcggcggttc ccatctccag
     6421 gctgtggcgc agcgcttctt gtgcgccttt gtcgccgcga ttcgggcgca gatcgattac
     6481 acgcgcaccg cccacgtgga cccacagccc cggtttcggc gccggccggc cagtttggag
     6541 cgcacgcatc attgcagcac cacctcctgc ccttctcgca agccggccag aacctctgtg
     6601 tttgcgccgt tgctgatgcc ggtctggata ggtattttgc gctttccggt tttttgcgac
     6661 gcgtcgggta cttccaccca ggtgctcttg tcccggtcat agagcacggc cgcttcggga
     6721 agcatgagca cgtttttgtg ttcctcgagg atgatctcgc cgttcgccgt cattagggcc
     6781 ttcaggtccc gcgggttcaa gatcgaaata cgcacctcga atgtggtgac gttgtccttt
     6841 tcctcgccca tgggtgatat tcgcgttact cttccctcat atttgcgctc tttgaaagac
     6901 tcgacagtga tgcgcgcggg ctgtccgagg tagaccttgc cgatatcgct ttcatcgacc
     6961 tttcctttca ggtagagttc ccgcatgtcg cccaaggtca tcaccagcgt ggccgccgac
     7021 cccatcacca agattgacga cacggcatcc ccgatctcaa cgtttcgcga aaggacgatt
     7081 ccatttagcg gcgagcggat cgtagcgtac tggaggtcct cgcgggcctg ctcgagcgcc
     7141 gccctcgatt cttttacgcg cgcttcggcc tggccgatct cggctctggc cgagttcgcg
     7201 ctggcgatag cttgttcctg ccggtttaca gccacttggt acgccttctc cgcgtcatcg
     7261 agggcctgag ccggaatcaa cccctcctta taaagaccct ggctgcgctc gaactgccgt
     7321 ttcaggaacg gcacatcgat gcctttcgcg tcactcagca ttttttcgta cgacgctcgt
     7381 gtggcgacca cattcgcctc ggaggcggcg agcgtcgctt ccagctgccg cacgcgggcg
     7441 ttcaactggt cgcggtcgag ctccgccaga acctggccct ccttcaccgt gtctcccaca
     7501 ttcaccagca ggcgttcgat gattccgctt gctttcgatt tgatttctac tctggcgatc
     7561 ggctcaattt tgccggtggc caccacgctg cgcgccaaat ctcctctttg gactcgcgcc
     7621 agctttgtgg gatcgagctt gacggaggtc ttggaaagag cggtaaaaag aaccactccg
     7681 cccccgatca gcacaagggc cgccgcaatg ccccaccagc cgctcttttt cttcttgttc
     7741 acgcggacgc ctttcctgac gcctctttat tgacgatcat tttataaata cggtagcagc
     7801 gacggtttgg ttccagtctt tgcgtaagaa gcctagtgat cctcggcggg ccgcgatgta
     7861 gcgaaacggc caaatcgcct gagaggccgg cttccggaaa cacggctgga ggcgtgatag
     7921 gatgatcgca atggaagatt ttccggtcgt tttccggttg atcttgctgc cgttgctgct
     7981 ggggatcaat ggttttttcg ccgccgctga agtcgctctg atttccgtcc ggctgacgcg
     8041 catcgctcag cttgcggaaa aaggaaatcc gagagcccgc gctgttgtgg atcttctggc
     8101 gaatcccgac cgcctccttt ccgcgacgca gctaggggtc acgctggcca gcctggggct
     8161 gggatgggcc ggcgaggaca ccgtgtaccg cttgatccta gcagtaatac catcaagctg
     8221 gccggccgtt tcaaccgccc tcagccatcc gcaatggcac cgcttgctgc atgtggcggc
     8281 gttcgtgatc gcctttttgg cgatcacttt tcttcacatg gtcgtcggcg aggtcgttcc
     8341 caaaaacctg gctttcgaga gggccgagcg cctcgcgctg gccgtcggcc cgactttgga
     8401 gtttttcgcc ggcgttacga attttttcat ccggatcgtc gagcgttcga gcgagtccct
     8461 gtcgcggctg ctcggcgtaa gaggaaagcc gcgtccggcc gcgcacaccg cggaagaact
     8521 gcggctgatc gtgaccgcga gccggcggca cggtgaactg ccgcggtcgc aggaggagat
     8581 gctgcacaac gtgatcgatt tttacgatct caccgtccgc caggtgatgg ttccgcgaaa
     8641 tgaaattgtc tccgtctccg ccgacgccac attcgaccag gttgtccgga cgatcgtgga
     8701 acatcagcac tcgcggctgc ctgtgtatga gaggactccg gaaaacatca taggaattct
     8761 ttacgctaaa gatttgtgga tgttccagcc gcgcgggcgc ggctttcggc tgcgcgccat
     8821 catccatccc cacaaggtgg tgccggagac caagccgctg caccaactgc tcgccgaatt
     8881 ccagcaggag cggttccaca tggccatggt ggtcgatgag tttggaacta tcgtggggct
     8941 ggtgactgtg gaagacctgc tggagcagat tgtgggtgaa attcgcgatg agcacgactt
     9001 gcccgagccg gcgcgggcgc cggcgagcgg ccccatcgag cttgacggcg ccaccaacgt
     9061 ccgggatctg gaaaatctat attccattca cgtgccttat gacgccggat tccagacttt
     9121 ggccggtttt cttctcgaaa agctcgggtc gatccctcgg ggaggcgagc aagtcgagca
     9181 tgagggccgg cgatacacag tggtggaaat ggaaggccgg cgcattgccc gcgtgcgtat
     9241 cgaagaaatc agtgaggaac cggcggggga ggaagcgcgg cctcgagaac ctctcgccga
     9301 acgctgaatt gcgtctcgct tcaggcgctt ccggttgcga tagaatccaa ctttcgtggt
     9361 tacctttgat ctcgccaact acccgaagcg aatcgccgaa gtgctggcaa aggcgggtcc
     9421 ggaagagcgg ccgggcgagc ggctgatgcc actggctttc ggaccgtgcg tctctcccgc
     9481 gtcgcgcgac cttctgcgca gcctcggcac agccgactta ttcgccccca gccaggtcgc
     9541 ggccgacgat tatgctgctg cggcgcgaag cggcctgttt gtatatctct cctgcatgga
     9601 cgacgcgcac gccatcgccc aggagattca caccgcaacc ggcagctact ggcacggtat
     9661 tttgcaccgc atggagccgg atttttcgaa cgcccgctat tggttccgcc gcgtcggccg
     9721 gcacgaaatt ttccctgcct tgcgcgccgc cgccgcggcc cttgccggcg aagatatacc
     9781 cgagttccgc aacgccgccc aatgggaccc ttttgccttc atcgatcttt gcgagcaaga
     9841 aggaggatct ggatcgggca agcgggcgaa ggcgctggag cgggtgcagc ttgccgaatg
     9901 gcaattgctg tttgatttct gctgccgccg ggcgttggga attccctggg gaaagtgaaa
     9961 cgatgagcgc gcgccatctc tgcctgcttg cccttctttt gctgtcaggt tgctcccatc
    10021 agcaggacac gccatacgat ctgatcatca gcggcggctc ggttttggac ggagatggcg
    10081 gcgaggcgat ccgcgccgat atcggggtaa gggacggcaa gatcgcaaag ctcggggact
    10141 tgtcccaggc tttcgcccac cggcggattc gcgccgaggg cctggttgtg gcgccgggat
    10201 tcatcgatat tcacaaccac tccgactata cgattctgca agagccgaag gccgaaagca
    10261 tgatccgcca gggcgtgacg acaatggtgc tcggcgaagg ggaatcggcc gggcccgtca
    10321 ggccgggatc ggcgcaaacc agaatcgagg gagtgaatgt ggattggacg acccttggcg
    10381 gctactttgc gcggcttgag cgccagcata tggcgaccaa tattgcttcc tatgtcgggg
    10441 aagggcaggt ctgggcgtgt gtgaagggag agacccaaag cccggctgct ccggccgagc
    10501 tggagcaaat gaagcggctg attgcccagg caatgcagga gggagcgatg ggcctttcta
    10561 catcgctgct catgccgcct tcgagccttg ccaccaccgg caacttgatc gagctggcgc
    10621 gaatggcaaa gcagtacggc gggatctatt ccagccatat ccgggacgaa ggggagggcg
    10681 tatttcgctc tgtggcggaa gccatcgctg tcggcagggg cgcccagatc cgcgtcgacg
    10741 tcattcacct gaagatcgcg cataagaatc tatgggggcg catgaacgag gtggcaggca
    10801 tgatccagaa ggcgcgggac gagggcctcg acatatgggc caacgtctat ccttatacag
    10861 ccggccaaaa caatctctcg gccatcattc cgccctgggc gcacgacggc ggcaatgaga
    10921 aaatgcttga gcggctgcgc gatccgaagc tgcgcgagcg catgcggcgc gaaatcctga
    10981 ccggcttgcc gggatggtat aaccactacc tcgcaaccgg cggcggctgg gccggcatgc
    11041 tattggtttc tttgaaggcg gacaggaata agccctttca gggcaaacgt atgagcgaac
    11101 tgatcgccgc ccgcggcggc gatcctgtca atgtcctgtt tgacgtgctg ctggaggaga
    11161 acggcagcgt cccgaccgtt tactttcatc attccgagcc ggatatggaa ctggcgctga
    11221 agcagccgtg gacctccatt ggctccgacg gcgcggccgt gagcccctac ggccccacca
    11281 gccacactca tccccatccc cgctactatg gcgcttttcc tcgcgtactc gggcgttacg
    11341 tccgcgaact gcacgtgctg tccctgccgg aagcggtgaa gaaaatgacc agcatgaacg
    11401 cggacaagct gggaatccgc gatcgggggc gcatcaagga agggaattgg gccgatatca
    11461 cgatttttga cccggagaaa gtcgccgatc aagccacgta tgaaaatccg caccagtatc
    11521 cactgggcat ctcgtacgtg atcgtcaacg gcgcggtaac gatcgacaac ggcaatcaca
    11581 cgggcgcgct ggccggcaaa gtgatttatg ggccgggccg caaataaggt caagccggcg
    11641 tgcgagccac ggccggcccg gctcggccgg cccgtattcg ccgccgggct gatctttctt
    11701 gccgcctgcg ctgtttacgg caattcgctg ggcgggcggt ttgttttcga cgacacgtcc
    11761 atcattgaaa gcaacccgca gatccggtcg ctaggatggg ccagccttga gcgcattttc
    11821 ggctcccatt attggcaggc ggtccagggg cagggagggc tatatcgccc cgtggttgtt
    11881 ttttcttacg cgctgaacta tgccgccgga gcgctcgatc cctggggcta ccacctcgtc
    11941 aacgtgcttt tgcacgggct gaatgcagcg ctggtattcc tgatttttca ggaactgttc
    12001 gaggaccgga ttttcgccgt ctggagcggc ttgctcttcg ccttgcatcc tattcataca
    12061 gaggcagtcg cgtacgtggt cggccgggcc gaaacgctgg ccgcgctgtt cttcctgata
    12121 gcatggctgt gctatttgcg ccggcgaatt ggggccgccg ccgccgcgtt ctttcttgcc
    12181 gttctcacca aagaaagcgc gtttacgttt ctggctgtcc ctccgctcag cgactacgtg
    12241 cgcgagcgcc gcctgacgcc gctgcgatac gcgccttttg cgggcgcggc ggggcttgcc
    12301 ctggccctgc ggtacgcagt gctggggggc gtcgcgccgt taagcatcaa tccgacatcg
    12361 aatcccctgg ccgccgccgg aaccgcggct cggctgctga cggcgacttg cgttttcggc
    12421 aaatatctct ggctgctcgt ttttcccgtg cctttgtcgg ctgattattc gttcaaccaa
    12481 atcccgccgg ttacaactcc atggaacctg tggtttgccg tttcagccgc cgcgctcgcg
    12541 ctgatggctg gggggatagt tttcgctttc cgccgctcgc cgccgttgtt tctgtgtgga
    12601 gcttttttct gcgctgcatt ttctctcacg tccaacttta tccggcccat cggaacgatc
    12661 atggcggagc gcctgctgta tctgccctcg ctcggtttta caggcgcgct cgggtatctg
    12721 atagcgcgcg ggtggcggtc gccccggcgc cgcgatatgg cgacagccgc gtccgtgctg
    12781 ctcgccgtgt tctatgccgg gcggaccgtt ttgagaaatg cggattggaa ggatcatctg
    12841 gcgctgtttt cgagcgccgc ggtggtctcg ccaaacagtt cgctggtcca ggccaatctg
    12901 gccagtgcgc ttctgtacca gaagggcgat gccgccggcg ccgccgagca cgcgcgcgag
    12961 gcgatccgca tcgagccggc cgaccccgcc gctcacatga cgctgggcga agcttgccag
    13021 cgcctgggcg atttacggca ggccatcgag gcttaccaag ctgtggagcg gctggcgccc
    13081 ggcaccgccg gagcgcacga ggcggcccgg cggaaacagg cgattttacg gtcgctgggg
    13141 cataacgcgc cgcagtaatc cttcctgctc gcgttccgaa atgctcgcgg aacctgtctc
    13201 cggcgcagga ggggcggccg gcgcggcaaa ttcgcagtaa aatgactgcg aatcctcggg
    13261 cgcgcggaga tgggaaagtc ccgcgccggc atttttggaa ctgggagcct gcggcgctgg
    13321 caagcatgcc cggagaattc aaaatctgcg gaaaggccgc ctgccgatga ggaaagcgtg
    13381 tgtaaacctc ttgttgggag cactagttac ggccggcttg ggaagcgccc aaaccccgtc
    13441 cgttgtattt agcgtcaagg cgtacggcgc gaagggcgac ggcaaaacgc tcgataccgc
    13501 cgccgtcaac aaagcgattg aggcggccag ttcggcgggc ggagggacgg tttactttcc
    13561 tccgggaacc tacgtttccg gttcgatcca cttgcggagc cgcgttacgt tgtttctggg
    13621 ccaagggtcc gttttggagg cctcgccgga tccggaagtc tacgatccga tcgaggagag
    13681 ccagaaacaa tgggccaaat tccaggattt cggccatacc tacttccaca acagcctgat
    13741 ctggggagag ggtctcgaag acatcgcgat cgtagggccg gggctgattt acggcaaggg
    13801 cttggcccgg gccgccgacc agcgcgtggg caataaggcg atctctttga agctttgccg
    13861 gaatgtaacg attcgcgacg tctccatgct aatgtgcggg catttcgcga tcctggcaac
    13921 gggcgtggac aacttgacga tcgacaacat caagatcgac acgaaccggg atgggatcga
    13981 cgtggattcc tgccgccatg tgcgcatttc gaattgcagc gtcaattcac cccgggacga
    14041 cgctatcgtc atcaaaggat cgcatgccct tggctttgca cgagccaccg aggacctgac
    14101 gatcaccaat tgcgaggtca ccggttttga agaggggagc tttctcaacg gcacttacaa
    14161 gcgccgcggc cccacgccgg agcgcaacgg tcccacgggg cgcatcaaga tcggaaccga
    14221 gtcggaaggg tccttcagaa acatcaccat ctcgaactgc gttttcgatt actcccgcgg
    14281 cctggcgctg gaaacagtgg atggagcgca gctggaagat atcagcatca ccaatatcac
    14341 aatgcgcgac atcgccaacg cgccgctcta tcttcgtctg ggccggcgca tgcgggcccc
    14401 ggaaggaact cccatcggcg ctttgcggcg ggtcaatatc agcaatctgg tggtctataa
    14461 cgccgacccg cgctacggat cgatgatcat cggcatcccc ggccgcgaca ttcaggatgt
    14521 gagcctgagt aacatccgca tttactatca agggggcggg acgaaggaac aggcggccat
    14581 caatccccct gagaatgaaa ccgagtatcc cgagccctac cgtcatggcg taatgcccgc
    14641 ctacggtttc ttcatccgcc acgcgcaggg cgtccagatg accaacgtcg aggtcagcta
    14701 cctgaaagag gatcttcgtc cggcgtttgt gctggaggat atcaagggcg ccgaattcaa
    14761 tctggtgaag gcccggcacg ctccgggcgt ccccactttc cggttgaaag gcgtcgagga
    14821 tttcagcctc caccgctgcg gcgcgctgcc ggacatgcgc ctgcccaagg cgcagcagaa
    14881 ggaattctag ccggcgcgca gtggtgcggg ccggggttga tcgcgagcag atagcggccc
    14941 ttcgcgggcg gcagacggct tgacagcggg agccggccgg gtatcttctg aaagacatga
    15001 gaagcagaaa cgcagcgttc cttgcagctt tcacgacc
//
LOCUS       DASREK010000191        13402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_426151, whole genome shotgun sequence.
ACCESSION   DASREK010000191 DASREK010000000
VERSION     DASREK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13402
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426151"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /gene="folK"
                     /locus_tag="VFA54_09870"
     CDS             complement(<1..255)
                     /gene="folK"
                     /locus_tag="VFA54_09870"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZS51157.1"
                     /translation="MSLNTIYLSLGSNLGDRMDHLRQAIEALPGGGVEVKRVSSIYET
                     EPVGVGGQPWFLNAVIEAETELFPIQLLDRLQSIEIQLGRR"
     gene            complement(415..1980)
                     /locus_tag="VFA54_09875"
     CDS             complement(415..1980)
                     /locus_tag="VFA54_09875"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZS51158.1"
                     /translation="MWSRQALQEFAREKFRDYKLIVVSNREPYIHQYQGGRIECLQPA
                     SGMATALDPLMRACGGVWVAHGSGDADRHAPGAMGRTPVPPDAPLYTLRRVWLSKELE
                     NGYYYGLANGGLWPLCHIAFRRPIFHREHWEAYRRVNEIFAHAVLEEAGGDPAFVFIQ
                     DYHLGLLPRLLKNRNPSLVVAQFWHIPWPNRETFRAFPWKEDLLDGLLGNDLLGFHLR
                     YHCQNFLDTVDRAIEARVDQERYEVTLRGKVTVVRPFPISIDFAEHASAAAGAAVDGE
                     MAHWRRALDLGAMRIGLGIDRIDYTKGIPERLCSLDLFLERCPEYRGKLVFVQIGVPS
                     RLRAPEYKKLGDEIEALVSEINAKWSSGAWRPILFLKTHLGPVQMMALHRLADFCMVS
                     SLHDGMNLVAKEYAASRFDENGMLILSEFTGAARELTDAILVNPFDLDSCAEAIRRAL
                     EMPPEERRKRMRRMRAVVADNNVYRWAAKILAALARIEAPQAALPMMDAAGQPNGEQL
                     CASVTASARPAGT"
     gene            complement(2144..3175)
                     /gene="ruvB"
                     /locus_tag="VFA54_09880"
     CDS             complement(2144..3175)
                     /gene="ruvB"
                     /locus_tag="VFA54_09880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZS51159.1"
                     /translation="MADRRIISADIQEEERQFEASLRPRTLADFIGQRNLKENLDIAI
                     QAAKARGEAMDHVLLYGPPGLGKTTLAAIVAAELGVKMDQTSGPVLQRKLDLTGVLTA
                     IQPRQVFFVDEIHRLMPDIEEMLYSALEDYRVDLMLGGGPGARTHSIPLPRFTAIGAT
                     TRAGLVSAPLRSRFGLVLRLNPYELADLQAVVRRSAKMLGVEIDEAGSEEIARRSRGT
                     PRIANRLLRRVRDYAEVRADGRITSDVARAALDMLEVDSFGLDEIDQKVMRTILEKYS
                     GGPVGLNTIAASIGEDANTIEEVYEPYLIQLGFLDRTPRGRIGTQRAFTYFKVAPRGF
                     TAALDFDSQ"
     gene            complement(3302..3889)
                     /gene="ruvA"
                     /locus_tag="VFA54_09885"
     CDS             complement(3302..3889)
                     /gene="ruvA"
                     /locus_tag="VFA54_09885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZS51160.1"
                     /translation="MIAFLRGRLIVKSPNQVVVDVNGVGYSVNIPVSTFSELPDRGAE
                     AELFVYTHVREDTLALYGFLTEKEKMLFERLISVSGIGPRLAITLLSGLQADELIAAI
                     RAGDVQRLVHVPGVGRKTGERLVLELREKMDFAAAAAGFAPARSSLEEDVISALLNLG
                     CARAAAQSAVQRARQNGAPAEFEPLFRAALKLAAG"
     gene            complement(4193..5311)
                     /locus_tag="VFA54_09890"
     CDS             complement(4193..5311)
                     /locus_tag="VFA54_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZS51161.1"
                     /translation="MKITDVEAIYLRLPQVKEQCDSGQDALIVKVTTDAGITGIGEVD
                     SAPLAVKGAIEGPFSHTTACGLRELLIGEDPFQTEYLWYKMYRKNIYGGRRGAGLHAM
                     SGIDIALWDIKGKALGQPIWKLLGGGFHKRIRPYASSLFGPTPKATGELARRFRDRGF
                     TAVKFGWDPMGRDEKTDIALVREARRGLGDEADLMIDAGLVWDAKTAIQRARAFADYD
                     IFWLEEPLLPDDYEGYRKLSEATDIRIAAGEEESNRLSFLDLMDRGRIDVVQVDLTRV
                     GGFTEAMKVASLAVDRGLPVVNHGFTTYINVAAALHFLNSIPNSFIAEFVVEEETTLR
                     DFITRQKFAAREGYLDIPDAPGLGIDLNEEGVATYRVA"
     gene            complement(5748..6314)
                     /locus_tag="VFA54_09895"
     CDS             complement(5748..6314)
                     /locus_tag="VFA54_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51162.1"
                     /translation="MTRYTPSRQYLPAAIVAFALAGFAGWCGWSWKPAFIPAFLFLVS
                     SGLLLFLATRPAIVIREGSWTVGDQSFLWPEVEQINTTGWHSPLILKIGLRGGRVLHL
                     IYPGDREMCSKLLRQMRRLARGARIDGVPYKQYWGERPTPAASEEVLSPPRYRVLRPE
                     DEEEVERLYNRLKSVGRLDHQTHEKLDD"
     gene            complement(6533..7087)
                     /locus_tag="VFA54_09900"
     CDS             complement(6533..7087)
                     /locus_tag="VFA54_09900"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZS51163.1"
                     /translation="MNPALLSQLWNIANQLTLFRLLVIPLILIAILYGRNGIALALFL
                     VAAVTDGIDGLVARRFNQRTTLGAYLDPIADKLLLSSSFFVLALVHSVPWWLTILVLS
                     RDVMILATSLVVVLSTQIRSFPPSILGKANTAVQVATVFCAVLQNAYPAGVLQSTLAL
                     LMWLTAGLTVASGVHYAARMSRKI"
     gene            complement(7084..7947)
                     /locus_tag="VFA54_09905"
     CDS             complement(7084..7947)
                     /locus_tag="VFA54_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZS51164.1"
                     /translation="MKTVAIVGVGLIGGSFALALRRNGFGGRIIGVSSPGTLEAARAR
                     GVIDEGSPLEEAAARADLIYLSHPIARILDQLPHVARLARPSALVTDAGSTKAAIVRR
                     ARECFGPQSAIFLGGHPMAGKAERGVEAADGNLFQGSTYVLTPSDASVFSLQPVQQFC
                     GWVETLGARIVTMDPDVHDRTVAFTSHLPQMASTALAAALFDTLEAGDAWTVAGGGLR
                     DMTRLARSAYELWRDICMTNTGNIERALSAYIQRLEHVRDNLRQPGLKEEFSKAARFA
                     ARLPKKQNNVT"
     gene            complement(7961..8989)
                     /gene="aroF"
                     /locus_tag="VFA54_09910"
     CDS             complement(7961..8989)
                     /gene="aroF"
                     /locus_tag="VFA54_09910"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZS51165.1"
                     /translation="MVIVMQEGAAEPQIQAVIDRLVARGFDVHRSTGARHTLLGAVGA
                     QIDFDTANLEVMDGVHEVLRISAPYKLASRAFRPEGAIVEAGDAKGVHARIGGPEVVL
                     MAGPCSVESADQINRISDLVAENGAKVIRGGAFKPRSSPYSFQGLGEEGLRLLREAAD
                     RNRLLVVSEVMDQSQIALVSAYADILQVGARNMQNFFLLRELGRVKRPVLLKRGISAT
                     IEEWLLSAEYILSGGNYQVILCERGIRTFENYTRNTLDISAIPVIHKLSHLPVIADPS
                     HGTGRRDKVSPMARAAVAAGADGLLIEVHQDPDHALSDGAQSLRPEQFSELVKQLRII
                     APAVGRTI"
     gene            complement(9026..9367)
                     /gene="pheA"
                     /locus_tag="VFA54_09915"
     CDS             complement(9026..9367)
                     /gene="pheA"
                     /locus_tag="VFA54_09915"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZS51166.1"
                     /translation="MDVSDWRRKIDAVDRKILELLNERARYVLALAPIKKRDGIPIYE
                     PKREEQVFANIAEHNGGPLSGEAVRRIFERIIDEMRSIQRGPMGEEPRAAELGRAQRP
                     APAQHAKKDPA"
     gene            complement(9376..10182)
                     /gene="trpA"
                     /locus_tag="VFA54_09920"
     CDS             complement(9376..10182)
                     /gene="trpA"
                     /locus_tag="VFA54_09920"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZS51167.1"
                     /translation="MTRISRRFTELKQADERGLVAYLTAGDPSLDRTPELVAALERGG
                     ADVIELGVPFSDPIADGPVIQRASERALRGGTSLRGVLDCVVEIRRRSEIPLVAFSYL
                     NPVLRYGFARFAADAAAAGLDGVLLTDLSVEEADPFVAEMRRQQLDTIFLAAPTSTAR
                     RLELAARYTTGFLYLVSRTGVTGERESLPDSVAPLAARIRALTRLPLAVGFGISRPEH
                     LAELAPLADAAAVGSAFVHLIERYQARPDLAKKLEEFTGQLKTGFRKIEV"
     gene            complement(10236..11480)
                     /gene="trpB"
                     /locus_tag="VFA54_09925"
     CDS             complement(10236..11480)
                     /gene="trpB"
                     /locus_tag="VFA54_09925"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZS51168.1"
                     /translation="MKQDCAAIELPGPDARGHFGPYGGRFVPEVLMAPLEALERAYDE
                     AREDPAFAAELDQLLRHYAGRPTPLYFARRLSEHLGGARIYLKREDLLHTGAHKINNC
                     LGQALLARRMGKRRIIAETGAGQHGVATATVCALFGLRGVVYMGEEDMRRQRLNVFRM
                     RLLGAEVIGVRAGSRTLKDAISEAMRDWVTNVADTHYLLGSALGAHPYPRMVRDFHSV
                     IGKEARRQILEMEGRLPDVLIACVGGGSNAIGLFSAFLQDAEVRLLGVEAGGRGPNLG
                     DHAARFRGGSPGVLHGAQSYLLQDEDGQVALTHSVSAGLDYALVGPEHAWLHDRGRAE
                     YTSVPDGEALEAARLLSRLEGIVPALESAHAVAEVIRRAPGAKGEIFVVNLSGRGDKD
                     MDIFRENFPELDEQRTNEIVNE"
     gene            complement(11529..12194)
                     /locus_tag="VFA54_09930"
     CDS             complement(11529..12194)
                     /locus_tag="VFA54_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZS51169.1"
                     /translation="MKHGTIIPLVPLEVIKVCGITRVEDALLAARGGATAIGLNFYPG
                     SPRYVDFGKGAILGGVIPRSILRVGVFVDETAERILETARAARLDVVQLHGSETPHDC
                     ELLAPLRVWKAFRVGENWSPQALAEYGCEAFLLDTASLDGSPGGTGRSFRWEIARQAA
                     RYGKIIIAGGLDGGNVAEAVRAGQPWGVDASSRIERSPGVKDPEKMRAYLEAASGARE
                     GGG"
     gene            complement(12322..13152)
                     /gene="trpC"
                     /locus_tag="VFA54_09935"
     CDS             complement(12322..13152)
                     /gene="trpC"
                     /locus_tag="VFA54_09935"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZS51170.1"
                     /translation="MAFPETRASELPILEQIVLRKRAELLLDQARTPLETLEQRVEQA
                     GGPAARRGFRRALERRAPAIIAEIKRASPSRGRIAERFEPAHIARQYEAAGASALSVL
                     TDKQFFEGSLEDLESARAASSLPVLRKDFTLDAYHLWQAAAHGADAVLLIVAILPQDQ
                     LRSLIERASDLALEAVVEVHDESELDRALEAGAELIGVNNRDLQTFEVSLETSLRLAR
                     RMPPGVLAISESGIRSAKDILRLSAAGFRAFLIGESLMRNPDPGAALASLLEGVHTAS
                     "
BASE COUNT         2421 a         4664 c         3913 g         2404 t
ORIGIN      
        1 gcggcggcca agctggattt cgatggattg gagccggtcg agcaattgga tcggaaatag
       61 ttccgtctcc gcttcgatca ccgcgttcaa gaaccatggc tgcccgccca cgcccaccgg
      121 ctctgtctcg tagatcgagg acacgcgctt cacctcgact ccgcccccgg gcaaagcctc
      181 aatcgcctgc ctcaggtggt ccatccggtc gccgagattc gatcccaagg agaggtaaat
      241 cgtattcaag gacaaatgtc agactgacat cgccagatta catcgaagcg ccggcccctg
      301 caacgccctg ccggcgcctg gcggccggat tgaggtggaa ctatccggag tctccggact
      361 caattcggtt ggttcgatgc cgcaaacgca gcccgtttcg cagcttgctg ccggtcatgt
      421 gccggcaggc cgcgccgaag cggtcacgct cgcacagagc tgctcgccgt tcggctgtcc
      481 ggcggcgtcc atcatcggaa gcgccgcctg cggcgcctca atccgggcca gggccgctag
      541 aatctttgcc gcccagcgat atacattgtt gtccgccacg acggcccgca tccgccgcat
      601 gcgcttcctt cgctcctccg gcggcatttc gagcgccctc cggatcgctt ccgcgcagct
      661 atccagatcg aacgggttta ccaaaatcgc atccgtaagc tcgcgggcgg cccctgtgaa
      721 ctcgctcaag atcagcatcc cattttcatc gaaccggctc gccgcgtact ccttcgccac
      781 cagattcatc ccgtcgtgca gggagctgac catgcagaaa tccgccagcc ggtgcagcgc
      841 catcatctgc accggtccca gatgcgtctt caaaaaaagg atcggacgcc acgcgcccga
      901 gctccacttg gcgttgatct ccgataccag cgcctcgatt tcgtccccca gctttttgta
      961 ttccggcgcc cgcaagcggc tcggcacgcc gatttgaaca aacaccagtt tcccccggta
     1021 ctccgggcag cgttcgagga atagatccag cgagcacagc cgttccggga tccccttcgt
     1081 ataatcgatg cggtcgattc ccaagccgat ccgcatcgcg cccaaatcca gcgcccgccg
     1141 ccagtgtgcc atctcgccat ccaccgccgc ccccgccgcc gccgaggcgt gctccgcaaa
     1201 atcgatgctg atgggaaaag ggcgcaccac cgtcaccttg ccgcgcagag tgacttcgta
     1261 gcgctcctga tcgaccctgg cttcgatcgc ccggtccacc gtatcgagaa aattctggca
     1321 gtgatagcgc aggtggaaac ccagcaggtc gttccccagc agcccgtcca gcaaatcctc
     1381 cttccacgga aaggcgcgaa acgtctcgcg gttcggccac ggaatgtgcc agaactgcgc
     1441 cacaaccagg cttggattgc ggttcttcaa aaggcggggc agcaaaccga gatggtagtc
     1501 ctgaatgaaa acaaacgccg ggtcgccgcc ggcttcctcc agaacggcgt gcgcgaaaat
     1561 ttcgttcacg cgccggtagg cttcccagtg ctcacggtgg aaaatcggac gccggaaggc
     1621 gatgtggcac aacggccaca ggcccccgtt cgctaggccg tagtagtatc cattttccag
     1681 ctctttgctc agccacacgc ggcgcagcgt gtatagaggg gcgtcgggcg gcacgggcgt
     1741 gcggcccatc gcccccggcg cgtgccggtc ggcgtccccg cttccgtgcg ccacccagac
     1801 cccgccgcaa gcccgcatca gcggatcgag cgccgtcgcc atcccgctgg ccggttgcag
     1861 acactcaatt cggcccccct ggtactggtg aatgtagggc tcgcgattcg ataccacaat
     1921 cagcttgtag tcgcgaaact tttcccgggc gaactcttgc aatgcttgtc tggaccacat
     1981 aattaaagct atttaatgaa caaccgttaa taatcgcaga gtttgtaggc aaagtccagc
     2041 tttttgtgat ccgcttcaca acggggcccc gccgcgttgg attgtccccc ctcgaaatcg
     2101 tggccgaacg tgggcaagcg gcgagcaaaa agggggacag gctctactgg gaatcgaagt
     2161 ccagcgccgc cgtaaagcct cgtggcgcga ccttgaagta agtaaaggcc cgctgggtgc
     2221 cgattcggcc gcgcggcgtg cggtccagaa atccgagttg aatcaggtag ggttcgtaca
     2281 cttcctcaat cgtgttggca tcctcgccga tcgacgccgc aatcgtattc agtccgaccg
     2341 gcccgccgga atacttctcc agaatcgtcc gcatgacttt ctggtcgatc tcatccaggc
     2401 cgaagctatc cacctcgagc atgtccaagg ctgcccgcgc cacatccgac gtgatgcggc
     2461 cgtcggcgcg cacttcggca taatcccgca cgcgccgcag caggcggttg gcgatgcggg
     2521 gcgtcccccg gctgcgccgc gcgatttcct ccgatccggc ttcatcgatt tctacgccca
     2581 gcattttcgc cgaccgccgc actacggcct gcaaatccgc cagctcgtaa ggattcagcc
     2641 gcagcaccag cccgaatcgg ctccgcagcg gcgcgctgac caggccggct cgcgtcgtgg
     2701 ccccaatggc cgtgaagcgc gggagcggga tggaatgcgt gcgtgctccc ggaccgccgc
     2761 ctagcatcag atccacgcgg tagtcttcga gcgccgagta gagcatctcc tcgatgtccg
     2821 gcatcaggcg gtggatctcg tccacgaaaa acacctgccg gggctggatc gccgtcagca
     2881 cgcctgtcag gtcgagcttc ctttgcagca cggggccgga agtctggtcc attttcacgc
     2941 ccaattcggc cgccacaatg gccgccagcg tcgtttttcc caggcccggc ggcccgtaga
     3001 gcaaaacgtg gtccatcgcc tcgccgcgcg ccttggccgc ctggatcgca atatccagat
     3061 tttctttcaa attccgctgc ccaatgaaat ccgccagcgt ccgcgggcgc agggaagcct
     3121 cgaactgccg ctcttcctcc tggatgtctg cggagatgat tcgacggtca gccatctcgg
     3181 actatttcat cacaatccgg ggcgagcccg tgcagttaaa ccccaggcgc aggttccgcc
     3241 cgctccgaac acgccgaaga tttttgcagg gattgcatgt gcagggagag atccccggga
     3301 gttagccggc cgccagcttg agcgccgccc ggaacagcgg ctcgaactct gcgggcgcgc
     3361 cgttctgccg ggctctttgc acggcgcttt gggcggcagc gcgcgcgcag cccaggttca
     3421 gcaaggcgga aatcacatcc tcctcgaggc tcgagcgagc cggcgcaaac ccggcggccg
     3481 cggccgcaaa atccattttc tcgcgaagct ccagtacgag ccgctccccg gttttccgtc
     3541 ccacgccagg cacgtgcacg agccgctgca cgtcgcccgc ccggattgcc gcaatcagct
     3601 cgtcggcttg aaggcccgat agcagagtga tggcaagtcg cggcccgatg ccgctcacgc
     3661 tgatcagtct ctcaaacagc atcttctcct tctcggtcaa aaagccgtag agagccagcg
     3721 tgtcctcgcg gacgtgcgtg tagacaaaca gctccgcttc cgcgcctcga tccggaagct
     3781 ccgagaaagt ggataccggg atgttgacgc tgtagcccac cccattgacg tccaccacga
     3841 cctgattcgg gctcttcaca attagccgtc cccgcagaaa cgcgatcatg ccagccgttc
     3901 tcccctttcc agaaatggaa taccctatga aagcacaaac gcgcctcgct agagacagcc
     3961 cgcgatcgca gcagctttac ggctctgcct cagcgtcaga ttgaaaagag caacagagag
     4021 gaccggaagt cccggtgcgc aggagttgct tctcgacacg gtgccgctga tcgaaatgga
     4081 tgtcaaaggc gctacggcgc gggcgctggg cgcgccacgg cctggatcgt agtgaccggg
     4141 acctgcgcca agacctgccc tgatcccgct ccgccctcct ctgccgcgat cgctaggcca
     4201 cgcggtacgt ggcgacgcct tcttcgttca gatcgatgcc cagtcccggg gcgtcgggaa
     4261 tatcgagata gccctcccgg gcggcgaatt tctgcctggt aatgaaatcc cgcaaagtgg
     4321 tttcctcttc cacgacaaac tcggcgatga aagagttggg aatcgaattt aaaaagtgca
     4381 gcgccgccgc cacgttgatg tacgtcgtga agccgtggtt taccaccggc agcccgcgat
     4441 ccaccgccag gcttgccacc ttcatagcct cagtgaatcc gcccacccgt gtaagatcga
     4501 cctgcaccac gtcgatgcgc ccgcggtcca tcaggtcgag gaacgagagc cggttgctct
     4561 cttcttcgcc tgccgcaatg cggatatcgg tcgcctcgga aagcttgcga tacccttcgt
     4621 agtcgtcggg cagcagcggc tcttccagcc agaaaatatc gtaatcggca aaagcccggg
     4681 cgcgctggat cgcggttttg gcgtcccaaa ccaggccggc gtcgatcatc aggtcggcct
     4741 catcccccag gccgcgccgc gcttcgcgca ccagcgcaat gtcggtcttc tcatcgcggc
     4801 ccatcggatc ccagccgaat ttgacggccg tgaaaccacg gtcccggaag cggcgcgcca
     4861 attccccggt ggccttcgga gtggggccga aaagagaact ggcgtagggg cgtatgcgct
     4921 tgtgaaaccc gccgccgagc agcttccaga tcggctgtcc gagcgctttg cctttgatgt
     4981 cccacagcgc gatgtcgatt ccgctcatcg cgtgcagacc cgcgccccgc cgcccgccgt
     5041 agatgttctt gcggtacatc ttgtaccaca aatactcggt ctggaacgga tcctcgccga
     5101 tcagcagctc gcgcaggccg caagccgtgg tatgcgagaa tggcccttcg atcgcgccct
     5161 tgacggcgag gggcgccgaa tccacctcgc cgatgccggt gattccggcg tccgtcgtca
     5221 ccttgacgat aagggcgtcc tggccgctgt cacactgttc cttcacttgc ggcagccgca
     5281 aataaattgc ttccacgtcc gtgattttca taagaggcgc atccggcctt ttctcctccg
     5341 cttgggccgg gaatcggctc gacaccgtcc tccgccgcgt tgccggccgc ctcgcttgcg
     5401 gctatgctta tccgctgcgc tgctctgtcg gggatcccgc aggcgtgctc catagtttga
     5461 cacaacacgg attgctgctg cgcaaagagc atgcgtcccc cggcgcgggc gcctggcccg
     5521 agagcaggga tacctgagcc gtgtccggcc gttacccgct gccttccgtt cacggtcggg
     5581 caggtctctc gttcaccaag ccggaacccg cttcggcaat tttgaaaagg gaaaatcttt
     5641 gatgggcgat ggaacgtgcg gccgccctgc ggctttgggc ctgtgccgcg ctgttttccg
     5701 cgtgctgaaa gccggtccgc ggaagaccgt ttcccgggtg tggccgctca gtcgtccagc
     5761 ttctcgtgcg tttgatgatc gaggcggccc accgacttca accggttgta cagccgctcc
     5821 acctcttctt cgtcctcggg ccgcagtacg cggtaacggg gaggggaaag aacctcttca
     5881 ctcgcggcag gcgtcggccg ctcgccccag tattgtttat aaggaacgcc gtcgatgcgc
     5941 gcgccgcgcg ccagccgccg catctgccgc agtagcttgc tgcacatctc cctatcgccg
     6001 ggatagatca ggtgcagcac ccggccgcca cgcagtccga tcttcagaat taaaggagag
     6061 tgccagccgg tcgtgttgat ttgctccacc tcgggccaga gaaagctttg gtcgcctacc
     6121 gtccacgatc cttcgcggat cacaatcgcg ggccgtgtgg ccagaaacag cagcagtccg
     6181 gaactgacca ggaaaagaaa cgccgggatg aaagccggct tccaactcca gccgcaccag
     6241 ccggcgaacc cggccagggc aaacgctacg atcgcggcgg gaaggtattg gcgggatggg
     6301 gtgtaccgag tcatgcaggc ggaagcgtct cctaacttct agcctaatcc cgttgcgggg
     6361 cttcgtcaat cgaaagtgta agttcctgga aaccacgaaa aagggcttcc tttcgccgct
     6421 tccgttggtt cccgcccata ccgccgccgg ccgtcaaaga ctcggccagc cagccaccga
     6481 aaagcccacc gggaggaaaa gaggaactcg cttcaccttc catcggggcg cgtcagatct
     6541 tgcgcgacat ccgggccgca tagtggaccc cgctggctac ggtcagcccg gcggtgagcc
     6601 acatcagaag cgccagcgtg gattgcagaa cgccggcggg atacgcattt tgcagtactg
     6661 cgcaaaatac cgtcgccacc tgcacagcgg tgttggcttt ccccagaatg ctgggaggga
     6721 agctgcggat ctgggtcgat agcacgacca ccaggctcgt cgccaggatc atcacgtcgc
     6781 ggctcaaaac caggatcgtc agccaccacg gcaccgagtg gaccagcgcg agcacgaaga
     6841 aactggaact cagcagaagc ttgtcggcga tcgggtcgag ataggcgccg agagttgtcc
     6901 gctggttaaa ccgccgggcg actagcccgt caatgccgtc ggtcacggct gccacgagaa
     6961 acaaagccag cgcgatcccg ttgcgcccgt aaagaatcgc gatcaggatg agcggaatca
     7021 ccagcaggcg aaacagcgtg agctggttgg ctatgttcca cagttgactt aacagcgctg
     7081 gattcacgtc acattgttct gcttttttgg cagcctcgcg gcgaaacggg ccgctttgga
     7141 aaactcttct tttaatccgg gctgtcgcag gttgtcccgc acatgctcca gccgctggat
     7201 ataagccgac agcgcccgct caatgtttcc cgtattggtc atgcagatat cccgccacaa
     7261 ctcgtaggcg ctgcgagcca gccgggtcat gtcacgcagg ccgccgccgg ccaccgtcca
     7321 ggcgtcgcct gcctccaggg tatcgaaaag cgccgctgcc agggcggtcg aagccatctg
     7381 cggcaaatgt gacgtaaaag ctacggtccg gtcgtgaacg tccgggtcca tggtcacgat
     7441 ccgcgccccc agggtctcca cccacccgca aaattgctga acaggctgga gcgaaaacac
     7501 cgaagcgtcc gaaggtgtca ggacgtacgt agatccctga aacaggttcc cgtcggccgc
     7561 ctccacgccg cgttccgcct tgcccgccat cgggtggccg cccagaaaaa tggcactctg
     7621 cggcccgaag cattcccggg cgcggcgcac gatcgctgct ttcgtgcttc ccgcgtcggt
     7681 gaccagggcc gagggccggg ccaggcgggc gacgtgcggc agttggtcca aaatgcgcgc
     7741 gatcggatgg gaaagataga tcaggtccgc ccgcgcggcc gcctcctcca gcggcgaacc
     7801 ctcgtcgatc acgccccggg cgcgcgccgc ttccagtgtg cctggcgagc tcacgccgat
     7861 aatgcggccc ccgaaaccgt tccggcgcag agccagggcg aacgatcctc cgatcaggcc
     7921 gacgccgaca atcgctaccg tcttcaatcc ccccgcgtcc tcagatcgtt ctgcccaccg
     7981 ccggcgcgat tatccgcaac tgctttacca gttccgaaaa ctgttcgggc cgcagcgatt
     8041 gcgcgccgtc gctgagagcg tggtcgggat cctgatgaac ttcgatcagc agcccgtcgg
     8101 cccctgccgc taccgccgcc cgcgccatgg gagatacttt gtcccggcgc ccggtgccgt
     8161 gcgagggatc tgcgatcacc ggcagatggg aaagcttgtg gatcaccgga atcgctgaga
     8221 tgtccagcgt gttccgcgtg tagttctcaa acgtgcggat gccccgctcg cacaggatca
     8281 cctgatagtt cccgcccgag agaatgtact cggccgaaag gagccactcc tcaatggtgg
     8341 ccgatatgcc ccgcttgagc aacaccggcc gcttgacccg ccccagttcg cgcagcagaa
     8401 aaaaattctg catgttgcgc gcccccactt gcaagatgtc ggcataggcg ctgaccagcg
     8461 cgatctggct ctggtccatc acttcgctca ccaccagcag ccggttgcgg tccgccgctt
     8521 cgcgcagcag gcgcagccct tcctccccta atccttgaaa actatacggc gagctccgcg
     8581 gcttgaacgc gcccccgcga atcaccttcg ctccattttc cgctaccagg tcggaaatcc
     8641 ggttgatttg gtcggcgctt tccacgctgc acgggccggc catcagaacg acttccggcc
     8701 cgccgatgcg cgcgtggacg cctttcgcgt cgcccgcctc cacgatggcg ccttccgggc
     8761 ggaacgcccg gcttgccagc ttgtaaggcg ccgagatccg cagcacttcg tggacgccgt
     8821 ccattacctc caggttcgcc gtgtcgaaat cgatctgcgc gcccacggcc ccgagcagcg
     8881 tatgccgcgc cccggtcgag cggtgcacgt cgaatccccg cgccaccagc cggtcgatga
     8941 ccgcctgaat ctggggctcc gccgccccct cttgcattac aatgaccatg cgggttcctc
     9001 tagccgcgtc aaaaaatatg gcgcattatg cgggatcttt cttggcatgc tgggcgggag
     9061 cgggccgttg cgccctgcca agctccgcgg cccgcggctc ttctcccatc ggcccgcgct
     9121 ggatcgatcg catctcgtcg atgatccgct cgaaaatgcg ccgcaccgcc tccccggaaa
     9181 gcggaccgcc gttgtgctcc gcgatgttcg cgaaaacctg ctcttcccgt ttgggctcgt
     9241 aaatgggaat gccatcgcgc ttcttgatcg gcgccagcgc cagcacgtag cgcgcgcgct
     9301 cattgagtaa ttccaggatc ttccgatcca ctgcgtcaat cttccgccgc cagtcggaga
     9361 catccatgcc ggtcgtcaga cctcgatttt ccggaagccc gttttcagtt gccccgtgaa
     9421 ctcttccagt tttttcgcga gatccggcct cgcttggtaa cgctcgatca gatgtacaaa
     9481 cgcgctgcca acggctgccg catcggccag aggagccagt tcggccaaat gctcgggccg
     9541 cgaaatgcca aagcccaccg ccagcggcag ccgcgtcagc gcgcggatcc gcgccgccaa
     9601 cggcgccacc gaatccggca gcgactcgcg ctcgccggta actccggtcc gcgagaccag
     9661 ataaaggaat cccgtcgtat accgcgccgc cagctccagg cgccgcgctg tgctcgtcgg
     9721 cgccgccaga aaaatcgtat cgagctgctg ccgccgcatt tcggcgacaa aaggatccgc
     9781 ctcctccacg ctcaaatccg tgagcaaaac gccgtccagc ccggcggccg ccgcgtcggc
     9841 tgcgaagcgc gcaaagccgt agcgcagcac gggattcaaa tagctgaacg caaccagcgg
     9901 tatctccgag cggcgccgga tctccaccac gcaatccagc accccgcgca ggctcgttcc
     9961 tccgcgcagc gcccgctcac tggcccgctg aattaccggg ccgtcggcga tcggatcgga
    10021 aaaaggcact cccagttcga tgacgtccgc gccgccgcgc tcgagcgccg ccaccagctc
    10081 cggcgttcgg tccagcgacg ggtcgccggc ggtcagatac gctaccagcc cgcgctcgtc
    10141 agcctgcttc aattcggtga aacggcggct aatgcgtgtc atcggcaatc gatggcggct
    10201 cccgtcctga ctcccggctt catccgatct ctaccctatt cgttaacaat ttcattcgtt
    10261 ctctgctcat cgagctccgg aaagttttcc cggaagatgt ccatatcttt gtctccccgg
    10321 ccggaaagat tcacgacaaa gatctcccct ttcgcgcccg gcgcccgccg gatcacttcg
    10381 gccacagcat gcgccgattc gagcgccggc acaataccct ccagccgcga aagcagccgc
    10441 gcggcttcga gcgcttcgcc gtccggcacg ctggtgtact ccgctcttcc ccggtcgtgc
    10501 agccaggcgt gctccggccc caccagcgcg taatccaggc cggctgaaac cgaatgcgtg
    10561 agagccactt gcccgtcctc gtcctgtaat agataactct gcgccccgtg cagtacgccc
    10621 ggcgagccgc cgcggaagcg cgccgcatga tcgccgaggt tcggcccccg cccgccggcc
    10681 tcgacgccca gcaggcgcac ctcagcgtcc tgcaagaacg cggaaaacaa cccaatggcg
    10741 ttgctgccgc cgcccacgca cgcgatcaga acgtcgggaa gccgtccctc catctccagg
    10801 atttgccggc gagcttcttt tccgatcacg ctgtgaaagt cgcgcaccat gcgcggatac
    10861 gggtgcgctc ccagcgccga gccgagcagg tagtgcgtgt cggcgacgtt ggtcacccag
    10921 tcccgcatgg cctcgctaat ggcgtccttc aacgtccggc tgcccgcgcg gaccccgatc
    10981 acctcggcgc cgagcagccg catgcggaat acattgagcc gctgccgccg catatcttcc
    11041 tcgcccatgt acaccacgcc ccgcagcccg aaaagcgcgc ataccgtggc cgtcgctact
    11101 ccgtgctgcc cggccccggt ctcggcgatg atgcggcgct tccccatgcg ccgggcgagc
    11161 agcgcctgcc cgaggcagtt gttgatcttg tgagctccgg tgtgaagcag atcttcccgt
    11221 ttcaagtaga tccgcgcccc cccgagatgc tcgctcagcc ggcgggcgaa atagagcggc
    11281 gtgggacgcc cggcatagtg ccggagcagt tggtccagtt cggcggcaaa tgcgggatcc
    11341 tcgcgcgctt cgtcataggc ccgctcgagc gcttccagag gcgccatcaa aacttcgggc
    11401 acaaaccggc cgccgtaagg gccgaagtgg ccgcgcgcat caggccccgg gagttcgatc
    11461 gccgcacaat cctgtttcat actttgccca gcctgcctgc gcccgggcga acggccgccc
    11521 gcgcagccct aaccgccgcc ttccctggcc ccgctcgccg cctccagata cgcgcgcatt
    11581 ttctccgggt ccttcacgcc cggcgagcgc tcgatccgcg aactggcgtc cacgccccac
    11641 ggctggcccg cccggaccgc ctccgcgacg ttgccgccgt ccaacccgcc ggcgatgata
    11701 atctttccgt accgcgccgc ctggcgggcg atttcccagc gaaagctcct gcccgtgccg
    11761 ccgggagagc cgtccaggct cgccgtgtcc agcagaaagg cctcgcagcc gtattccgcc
    11821 agcgcctggg ggctccagtt ctcgcccacc cgaaacgctt tccacacccg cagcggcgcc
    11881 agcaattcgc aatcgtgcgg cgtctcgctg ccatgcagtt gcaccacatc cagccgggcg
    11941 gcgcgggccg tttccagaat gcgctcggcg gtctcgtcta cgaagacgcc tactcgcaat
    12001 atgcttcgcg ggatcacgcc gcctagtatc gcccctttgc cgaaatccac gtagcgcgga
    12061 ctgccgggat aaaaattcag accgatggcc gtagcgccgc cgcgcgccgc cagcagcgcg
    12121 tcttcgacgc gcgtgattcc gcagaccttg atgacttcca ggggcacgag aggaattatc
    12181 gttccgtgct tcatcctatc gcacccggac gcgctttgcc atgccgcgcc gcccggccgg
    12241 ccctcgacaa acgcagcgaa gcgcctcgcc ggaagtacgg ccgatgcgcc cttcagcgcc
    12301 gccttccatt tcggtagtct gtcacgaagc ggtgtggacc ccctctaaga gcgaagccag
    12361 agccgcccca gggtcgggat tgcgcatcag actttcgccg atgagaaacg cccgaaaccc
    12421 cgccgccgaa aggcggagaa tgtctttcgc gctgcgaatg ccgctctcgc tgatggcaag
    12481 cacgcccggc ggcatccggc gcgccagccg cagcgaagtt tccaaggaca cttcgaaggt
    12541 ctggagatcg cgattgttta ccccgatcaa ttcggccccg gcctccagtg cgcgatccag
    12601 ttcggattcg tcgtgcacct ccaccacggc ctccagcgcc agatcggagg cgcgctcgat
    12661 caggcttcgg agctgatcct gcgggaggat ggcgacaatg agaagaaccg cgtccgcccc
    12721 gtgcgccgcc gcctgccaga ggtgatatgc gtccagcgtg aaatccttgc gcagcacggg
    12781 caggctggag gctgcccggg cgctttccag gtcttcgagc gacccctcga aaaattgctt
    12841 gtcggtcagc actgagagag cgctcgcgcc ggccgcctca tattgccgcg caatgtgagc
    12901 cggctcgaac cgctccgcga tgcgccctcg cgagggcgag gcccgcttga tctccgcgat
    12961 gatcgccggc gcccgccgct cgagcgccct ccggaatccc cgccgggcgg ccgggccgcc
    13021 ggcctgctca acgcgctgct cgagcgtctc gagcggcgtc cgggcttggt cgagcagcaa
    13081 ttcggcgcgt ttccggagaa cgatttgctc cagaatcggc agctccgagg cgcgggtctc
    13141 cggaaaggcc attgcatctt catcctatcg cacgactgcg cgctgcgcca ggcgcgggcc
    13201 tcggcgcccc agccgcgcgc aatccggaat ctcgtcgtgc gagggcgggg aaatacggga
    13261 agcgcctccg tctgcaagat gcaaactccg tccatggcgc tgcgcccgcc gctcgccttt
    13321 tgtgggatcg cggcaatggg gataaagtaa ttagaacagc cggagcgcat atgcttcctt
    13381 ttctcggcaa aaaaagccgg ac
//
LOCUS       DASREK010000192         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1573533, whole genome shotgun sequence.
ACCESSION   DASREK010000192 DASREK010000000
VERSION     DASREK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573533"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VFA54_09940"
     CDS             <1..1372
                     /locus_tag="VFA54_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51171.1"
                     /translation="FGDLFSLAFIAAAMITLSPSLTLAAVSVSPLVIAATFLFRRAAR
                     DANRRIRVAIAKINSYLQEHVTGMAVVQLFNHEKRSRERFAAINADHRDAYKDAILAH
                     SLFYPAIEWLGTLAMATLLIYGGYRILAGKMTIGVLVAFMQYSLRVFRPIQDLGEKYN
                     ILQSAMASAERIFKLLDTPVRIQAPARPAPLRERKARIEFQNVWFAYNGEEWVLKDLS
                     FTVEPGETVALVGHTGAGKTTILSLLLRFYDVSRGRVLIDGIDVREFDPVELRRRFGV
                     VLQEPYLFTGTIATNIRLGTESIPYERVEEAAEEVNLADFIRSLPAGFHEEVRERGSS
                     LSTGQKQLINFARALAHDPQFLILDEATSSVDTETELRVREALSRIVTGRTSIIVAHR
                     LSTIQRADRILVMHKGRLRESGSHQELLAQRGIYWKLYQLQYKDQEVRLAPPETGLRP
                     GLEPRV"
     gene            1440..2894
                     /gene="guaB"
                     /locus_tag="VFA54_09945"
     CDS             1440..2894
                     /gene="guaB"
                     /locus_tag="VFA54_09945"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZS51172.1"
                     /translation="MLNSPLPEALTFDDVLLEPARSSVLPAEADTRTCLTRKIALNIP
                     VVSAAMDTVTESRLAIALAQQGGMGFIHRNMPIERQAEEVDRVKRSESGMIVDPVTMA
                     PENKISEALEVMKRFRISGVPITKHGKLVGILTNRDLRFETRYDLPISEVMTKTNLVT
                     VPVGTTLDEAEKILHRHRVEKLLVVDDEYNLKGLITVKDIQKKLKYPNAAKDSQGRLL
                     VGAAIGAAGDYLERALELARKKVDVLAIDTAHGHSERVMEAARQVRRRLPDIQLVAGN
                     VATEKGAADLIRLGVDAVKVGIGPGSICTTRVVSGAGVPQITAIAECARAARGTGVPI
                     ISDGGIKYSGDITKAVAAGANAVMIGSLFAGTEESPGETILYQGRIFKSYRGMGSLGA
                     MMAGSSDRYAQSGDGKLVPEGIEGRVPSKGPLVELVHQLVGGLKAGMGYCGCANIQEL
                     QEKVRFLRISPAGVRESHVHDVIITKEAPNYQVE"
     gene            3016..4185
                     /gene="ada"
                     /locus_tag="VFA54_09950"
     CDS             3016..4185
                     /gene="ada"
                     /locus_tag="VFA54_09950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HZS51173.1"
                     /translation="MQDRIFWRAVLARDPRFNDRFVYAVRSTGVYCRPICPSRRPARD
                     QVLFFSGPGAAEQAGFRPCRRCHPRQPADPLVERTKQICRYMEANGEERLTLAGLASR
                     AGVSARQLQRIFKRATGITPREYAEALRLGRVKKALKKGDDVTTALYKAGYGSSSRLY
                     ERSSAQLGMTPAVYGRGGAGMKISYTIAASRFGRVLVAWTERGICALSFGGAGKPLQA
                     FLTEEYPKAEIRRHHGAMTGFVQDVLAHLQGRQPHIDLPVDVQATAFQRRVWQELQSI
                     PYGETRTYSQIARSLGQPAAIRAVARACATNPVSIVIPCHRVIRQDGSLGGYRWGLGR
                     KRALLAQERQARAGEGTNRENETSAQQAAMRRPAADPLPNRRQAPAGSATSGGLA"
BASE COUNT          888 a         1394 c         1373 g          835 t
ORIGIN      
        1 cttcggcgat cttttttcgc tggcgttcat cgccgccgcc atgatcacct tgagcccaag
       61 cctcactttg gccgcggtca gcgtctctcc cctggtcatc gcggcgacct tcctgttccg
      121 ccgagcggcg cgagatgcca atcgccgcat acgggtagcg atcgccaaaa tcaactctta
      181 cttgcaggag cacgtcaccg ggatggccgt agtccagctt ttcaatcacg aaaagcgcag
      241 ccgcgagcgg ttcgccgcca tcaatgcgga ccaccgtgac gcttacaagg acgccattct
      301 ggcccatagc ctgttttatc cagccataga atggctggga accctcgcta tggcgacgct
      361 gctcatctac ggcggctatc ggatcctcgc cgggaaaatg accattggcg tgctggtggc
      421 cttcatgcag tattcgctcc gcgtattccg ccctatccag gatctcggcg agaaatacaa
      481 tatcctgcaa tcggctatgg ccagcgccga gcgtattttc aagctgctgg atactccggt
      541 gcgaattcag gcgcccgccc ggcctgcgcc cttgcgcgaa agaaaagctc gcatcgagtt
      601 tcagaacgtt tggttcgcct acaacggcga agagtgggtg ctcaaagact tgagcttcac
      661 cgtagagccc ggggaaaccg tcgcgctggt gggccatacc ggagccggca agacaaccat
      721 cctcagcctg ttgctgcgtt tctacgatgt aagccgcggg cgcgtgctga ttgacggcat
      781 tgacgtcagg gaatttgatc ccgtcgaact gcgccggcgg ttcggcgtgg tattgcagga
      841 gccgtacctc ttcacgggga ccatcgccac gaacatccgg ctgggcaccg aatccatacc
      901 ctatgagcgc gtggaagagg cggcggaaga ggtaaatctg gccgacttca tccgctcgct
      961 gcccgcggga tttcacgagg aggtccgcga gcgcggcagc tccctttcca cggggcagaa
     1021 gcagctcatt aatttcgccc gggcgctggc ccacgacccg caatttctga tcctggacga
     1081 ggcgacgtcc agcgtcgata cggagactga gctccgcgtg cgcgaagctc tcagccgcat
     1141 cgtcaccggc cgtacgtcga tcattgtggc ccaccggctt tcgacgattc agcgcgccga
     1201 ccggattctg gtgatgcata aaggacggct gcgggagagc ggctctcacc aggagctgct
     1261 ggcgcagcgc ggcatctatt ggaagctcta ccagctccag tacaaagatc aggaggttcg
     1321 cctggctccg ccggaaacgg gcctgcggcc aggcttggag cctcgagttt gacctgggcg
     1381 cgagcgcaca gcccaaggga tgatggctgg cgcccgcatg gcttagacta tactggtata
     1441 tgctgaattc tcctctcccc gaagccttga catttgatga tgtgcttctc gagccggcgc
     1501 gcagcagtgt gctgccggct gaggcggata cacgcacctg cctgacgcgc aaaatcgcgc
     1561 tgaatattcc cgtggtcagc gcggccatgg acacggtcac cgaatcccgg ctcgctatcg
     1621 cactcgccca gcaggggggc atggggttca ttcaccgcaa tatgccgatt gagcggcagg
     1681 ctgaagaagt ggatcgggta aaacgcagcg agagcggaat gatcgtggac ccggtgacga
     1741 tggcgccgga aaacaagatc agcgaagctc tcgaggtcat gaagcgcttt cgcatttccg
     1801 gcgtgcccat caccaaacat ggcaagctgg tcggcattct cacgaaccgg gacttgcggt
     1861 tcgagactcg ctacgacctc cccatctccg aggtaatgac aaagacgaac ctggttaccg
     1921 ttccggtggg caccacgctc gacgaagctg agaaaatcct gcatcgccac cgcgtcgaga
     1981 agctgctggt cgtggacgac gaatacaatc tcaagggtct catcaccgtc aaagatattc
     2041 agaaaaagct caagtacccg aatgccgcca aggattctca ggggcgcctg ctggtgggcg
     2101 cggcaatcgg agccgccggc gattacctgg agcgcgctct ggagctcgcc cgcaaaaagg
     2161 tggacgtgtt agctatcgac acggcccacg gtcatagtga acgggtgatg gaagcggccc
     2221 ggcaggtgcg ccgtcgcctg cccgatatcc agctcgtggc cggcaatgtc gccacggaga
     2281 aaggggccgc cgatttgatt cgcctcggcg ttgacgcagt caaggtcggc attggaccgg
     2341 gatcgatttg caccacccgc gtggtcagcg gagccggcgt cccgcaaatc accgcgatcg
     2401 ccgagtgcgc ccgcgcggct cgcggtacgg gtgtgcccat catttccgac ggcggcatca
     2461 agtattccgg cgatatcact aaggctgtcg ccgccggcgc caatgcggtg atgataggca
     2521 gcctgttcgc tggcacggaa gaaagtcccg gagaaactat cctgtatcag gggcgcatct
     2581 tcaaaagcta ccggggcatg ggttcgctgg gcgcgatgat ggccgggtcg agcgaccgct
     2641 atgcgcagtc cggcgatgga aagcttgttc ctgagggcat cgagggccgc gtgccatcca
     2701 aggggccgct ggtcgaacta gtacatcaat tggtgggggg gctcaaagcc ggaatggggt
     2761 attgcggctg cgcgaacatt caggaattgc aagagaaggt tcggtttctc cgcatttctc
     2821 ccgccggggt ccgggaaagc cacgttcacg acgtgatcat caccaaggaa gcgccgaatt
     2881 atcaggtaga gtagcggccc cgcgccgggt tgcgaacatc accggggctg tctggagtac
     2941 acagccagtc cacattgtta atttgaaggc gtgatgcgga tagatctttc cccgccgctg
     3001 cccgaagctc aaagtatgca ggatcgaatt ttctggcggg ccgtcctggc tcgggatccc
     3061 agattcaatg accgcttcgt ctacgcggtg cgctcgaccg gcgtttactg ccgtcccatt
     3121 tgcccgtccc gcaggccggc tcgcgatcag gttctatttt tttccgggcc gggagccgcc
     3181 gagcaggctg gattccgtcc ttgccggcgc tgccatcccc gccagccggc cgatccgctt
     3241 gtggaacgga caaagcaaat ctgccgctac atggaagcca atggcgaaga acggctgact
     3301 ctagccggcc tggcctcccg cgcgggagta agcgcgcgcc aactgcaacg catatttaaa
     3361 cgcgccacgg gaatcacgcc gcgcgagtat gctgaggctt tgcggctcgg ccgggtgaag
     3421 aaagccctca agaaaggaga cgacgtgacc acagcgctat acaaggccgg ctacggctcc
     3481 agcagccggc tctacgagcg gtcctccgcc caactaggaa tgaccccggc cgtctacggc
     3541 cgcggcggcg ctggaatgaa aattagctat accattgccg cttcgcgatt cggccgcgtt
     3601 ctcgtcgcct ggaccgagcg cggcatctgc gcgctgagct tcggcggcgc tggcaaacct
     3661 ctgcaagcct ttctgaccga ggaatacccg aaggccgaga tccgccggca tcatggcgcc
     3721 atgaccggct tcgtccagga cgtgcttgcg catctccagg gccgccagcc ccatatcgat
     3781 cttccggtcg atgtgcaggc taccgcgttt cagcgccggg tctggcagga attgcaatcc
     3841 attccgtacg gcgagacgcg cacctacagt cagatcgccc ggtcgctcgg ccaaccggcc
     3901 gctatccggg ccgtggctcg cgcgtgcgct acaaatcccg tttccattgt cattccctgc
     3961 catcgcgtga ttcgccagga cggcagcctg ggaggttatc gctggggtct cggccgtaag
     4021 cgggcgctgc tcgcccagga acggcaggct cgagccggag aaggcacaaa ccgggagaat
     4081 gaaacgtccg cacagcaggc ggcaatgcgg aggccggctg ctgatccgct cccgaaccgc
     4141 cgtcaagctc ctgcgggctc agcgacctca ggcggtttgg cttgaattac aaggaggctc
     4201 caaaagtccc aacattcggc tgacgcctgc ggcgatgaag ccagctctct cgatgcgcag
     4261 cgcggcaagg cctatactgt tggcatccgt ctacgcgaaa atggagattc cagcccgtag
     4321 aacggataag caacatgaag cgaatttccg gctgcctcac cacacttatc gcgctgctcg
     4381 ccggcgtctg cgccgcccag gagcatcatg cgatgggagg atcgagccag ccagcgtctc
     4441 ttctggctgg cttgggcaac cagcaccacc cgatctccac gaagaacata
//
LOCUS       DASREK010000193         9416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_920391, whole genome shotgun sequence.
ACCESSION   DASREK010000193 DASREK010000000
VERSION     DASREK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920391"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1291)
                     /locus_tag="VFA54_09955"
     CDS             complement(26..1291)
                     /locus_tag="VFA54_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51174.1"
                     /translation="MEPRSSDPAARVAFRYSSFRYYMFARFLTTTSSEMQAVAVAWQV
                     YAITNRPLDLGLVGLAHFLPGVFLFLIAGHTADRVPRRRILQLCYAAFSVCSLLLLAL
                     ALRGGASVFPVYAVLLLNGMVRSFNGPAGQAFLPLLVPEKHFPNAVAWGSSIFQAATI
                     IGPMAGGVLYGWTGSPAPVYGCAALEYLAALVLVSAIKIKAAAERGRRAEPAAAVLQG
                     LQYIWRNKLILGAISLDLFAVLLGGAVALLPVYAREILNVGARGLGALRSAPGIGAVI
                     TAVLVAHWPVRRRAGAAMLGCVFGFGAFTIIFGLSRSFPLSLVVLGLAGACDMVSVIV
                     RQTLIQLATPDTMRGRVSAVNMLFIGASNELGQFESGVTAQWLGTIPAVITGGAGTIA
                     VVIAWARLFPALRRVDELTPAPAAVKAAG"
     gene            complement(1506..1904)
                     /locus_tag="VFA54_09960"
     CDS             complement(1506..1904)
                     /locus_tag="VFA54_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51175.1"
                     /translation="MRFFTPLLIITLAGTMFSAAVAKDHGKHGREVEFDREHHEKYRD
                     ACFTEEHVRIIREYYHPRSLPPGLEKKLYRTGHLPPGWKRRIRPFPVVIEQRLPPVCA
                     GCVRGYMDGYAIVYQPQSRLIIDVHALIGL"
     gene            complement(2061..2561)
                     /locus_tag="VFA54_09965"
     CDS             complement(2061..2561)
                     /locus_tag="VFA54_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZS51176.1"
                     /translation="MRAGLSFGDLLREIARTLAAYIRGEIIIALTMSALYAVAFGSLK
                     VPAWPLIAVLCGFLHLVPNFGVLIGFVLAVGLTAIAGREFWRVAAVAGAFVLFQGFEG
                     YYLTPHILGRRLGLRPLAVFLAVLAGSMLFGVVGLLAAAPALAIANVLWKFFRSRSSD
                     SGHGPH"
     gene            complement(2702..3430)
                     /locus_tag="VFA54_09970"
     CDS             complement(2702..3430)
                     /locus_tag="VFA54_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51177.1"
                     /translation="MNADILRRLERLRTDTVSGATNLAVSAIDIALDAPEDWPQLARE
                     FRSMHPAIVTVANVGRLLESQNPDLPGLRRSLLEGNQRIAPRLAALLPPGARILTISR
                     SSTVEAALAGRGSSVLVLESLPGGEGRLLAESLNAKLIPDAFMGQAAARVDCALVGID
                     AFDADGAILHKTGTLPLALCCAHFGKPFYAAGHSFKLAPGRTADLLASRDEFTLFDRI
                     PPELITRIITERGNFERGKLLGEG"
     gene            complement(3448..5646)
                     /locus_tag="VFA54_09975"
     CDS             complement(3448..5646)
                     /locus_tag="VFA54_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51178.1"
                     /translation="MRSYFLLALILASPALAETTRTWEQSRFDEFEKGMIEHVALRSD
                     GKLGLAPRFQEIYDAPLAYLWALARDSKGNLFAGGGPGGRVFKITPEGKKTTYFDTEA
                     LEIHSLAIDRRDNLYAATSPDSKIYKIDPSGRVSLFVDPKVKYIWAMAFNSQGDLFVA
                     TGDQGRVLRVSPSGKATVFFKTEEAHIRSLLIDKNDDLVIGTDPGGLVIRIPGSGGPG
                     FVLYQSSKKEITALALAPDGMLYAAGVGSRGSGGVTPLAVPAAPAPAPAPVGNLLGGA
                     PAPAGPAPAPQPASLPSSLRSGVSGGSEVFRIGLDGEPRRVWSSTEDIVYTLGFNPKG
                     RLLIGTGNRGRVFQIEADHVYSLLVKAAPTQVTAFLQGPDGVVFAATGNIGKIYRLGP
                     ELESRGTFESEVFDAGMFAHWGRLTWTGSVPPNTSLTLYARSGNLVTPDQYWSSWSKP
                     VTAMDGAPLDCPPARFVQWKAVLETRSGDSPLLDSVEIAYQPKNVAPTILQLEITPPN
                     YKFADPSAASPSTQTLSLPALGSRPSTKRGSAPAAPQAPRSMNRAKGYLGARWLAEDD
                     NNDELVYKVEIRGVKEQNWKLLRDKVEQPYISWDSTAFADGQYQLRVTASDAPSNPGP
                     EALIYSLESEPFYIDNTPPAISGLTAAVENGRVRVRFHVADAISNIDNSEYSMDGGEW
                     RVMLPATRLFDSKELDYDFLTDSVAGGEHTIAIRVWDSNDNLATAKALVQ"
     gene            complement(5712..7430)
                     /locus_tag="VFA54_09980"
     CDS             complement(5712..7430)
                     /locus_tag="VFA54_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51179.1"
                     /translation="MKLADVKPGMKATAWTVFSGSEPEPVPVEIIGIMKNVWGPRQDI
                     ILGKMGGKAQRTNVAAGMSGSPVYYEGKLIGAVSLRLGAFSPDAICGITPIELMLEIN
                     EFDQSRPAGASTPERAAAAKKVAVTPGLLRPASDELYLTPIQTPLTFGGFNEAVLHDF
                     AGIFDDLGMVAVQGGGSAGTPARIAPSAATALQPGDAISGVLVSGDMSIAASGTVTYN
                     DGKRILAFGHPMFNTGPVEMPMAQAEVVHTLASSFTPTKIMNFGEFVGALRQDRHSGI
                     MGVLGEQAPTIPVSVRVRSFADSRAVRAEKDFHFQVFQNQKWTPQLMMFTLYNSMFGV
                     NDFAEESTFRLSGNIQLDGRQKISLSTMRTSGDTPVPAPMLLAGWFGDKFNRLFTNSV
                     KMPHLRSVDVTIDLLPERRVAVIENAWTEKSEVEPGDELVGKVFLRPYRGQRIEKQFR
                     IKVPAGVQKGTLRLMVSDADTVNRNKYAAGGNRLMDLPETVSLINQELSNNQVYISLM
                     QPTVTAYYDDKTLPNVPSSVLNVIEAGNERNRRLMLANESSVEQTAIPFDYVVSGSYS
                     LNVTVR"
     gene            complement(7740..8372)
                     /locus_tag="VFA54_09985"
     CDS             complement(7740..8372)
                     /locus_tag="VFA54_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZS51180.1"
                     /translation="MKRFAAHLYTLLFHLGGFGALGLGILDSSFLVMPLANDILIVGL
                     TARRHMMLPYYAFMASAGSVLGCALTDAISRKGGEEGLGKHVSPRRLEYVKKKVKKSA
                     AWALAVASLMPPPFPFTPFVAAAAALQYPRQKLLTVIGISRFIRFSAVGLLAVFFGTR
                     ILELAKSPVVQGGILAIIALSVGASAVSIYSWVRRSRGSTAASARKNRAA"
     gene            complement(8415..8708)
                     /locus_tag="VFA54_09990"
     CDS             complement(8415..8708)
                     /locus_tag="VFA54_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51181.1"
                     /translation="MRARLNTQNPCGACHRGLEQATHVSKADMPQMADCLVCHSEIEP
                     PFTCEKCHSDTVALKPANHTADWLERHSRRSVKFDRASCRICHGVRFRCMGCH"
     gene            complement(8996..>9416)
                     /locus_tag="VFA54_09995"
     CDS             complement(8996..>9416)
                     /locus_tag="VFA54_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HZS51182.1"
                     /translation="YGWPYAYIGPHEDPRHKGERPDLVKKTIVPDVLLGSHVAVLDFI
                     FYTGQQFPKQYQGGAFLAFHGSWNRSKRTGYSVAFVPFQNGKPSGGPQDFLTGWMLSP
                     NRREVWGRPVGLLQLPDGSLLVSDDGGNKIWHITYKG"
BASE COUNT         1932 a         2981 c         2814 g         1689 t
ORIGIN      
        1 cttgtccgca aaactctttg ccgggtcagc cagccgcctt cactgccgcc ggcgcgggcg
       61 tcagctcgtc gacgcgccgc aacgccggaa acagccgcgc ccacgcgatc acaacggcga
      121 tcgtgccggc gccgccggta atcacggccg gaatcgttcc gagccactgc gccgttactc
      181 cggattcgaa ctggcccagt tcgtttgacg ctccgatgaa gagcatgttt acggcgctga
      241 cgcggccccg catagtgtcg ggcgtggcaa gctggatcaa ggtttgccgc acaatgacgc
      301 tcaccatgtc gcaggctccg gccagtccca aaaccactaa agagagcgga aaactacggg
      361 agaggccaaa gatgatcgtg aatgccccga agccgaacac gcagccgagc atcgccgcac
      421 ccgcccggcg gcggacgggc caatgggcca cgaggacggc cgtaatcaca gctccaatac
      481 cgggcgcgct gcgcagggcg ccgagtcccc gggcgcccac gttcaggatc tcccgcgcat
      541 acacaggcag caaggctaca gcgccgccca gtaacacggc gaaaagatcc agcgaaatcg
      601 cgcccagaat cagcttattg cgccagatgt attgcagtcc ttgcagtaca gcggccgctg
      661 gctccgcccg ccgcccgcgt tccgccgccg ctttgatctt gatggctgac accagcacca
      721 atgctgccag gtattccaac gccgcacagc cgtagacggg agcgggactt cccgtccagc
      781 catagagaac tccgccggcc atggggccga taatcgtcgc ggcctggaat atcgacgatc
      841 cccaggccac ggcgttgggg aagtgcttct cgggtaccag gagcggcaaa aaggcttgac
      901 ccgcgggacc attgaaggag cgcaccattc cgttgagcaa taacacggca tagaccggaa
      961 aaaccgaggc gcctccgcgg agcgccaggg cgagcaacaa cagggagcaa accgaaaacg
     1021 ccgcatagca gagttgaaga attcgccgcc gcgggactcg gtcggctgtg tgtcccgcaa
     1081 tcaggaaaag aaacacgccc ggaagaaaat gggccagccc caccagtccc agatccaggg
     1141 ggcgattcgt gatcgcatag acctgccagg ccacagcgac cgcctgcatc tccgacgaag
     1201 tggtcgtgag aaagcgggca aacatgtaat agcggaagct cgaataacgg aaggcgacgc
     1261 gggccgccgg atcactggac ctcggctcca ttcggttatg aaaagccagt ttcgcacaga
     1321 ggcgccatcg gccgcttcgg tctgccttct ccggccggga caggcagcgg cgcgcccgac
     1381 aacgaggcgc gcaaaaggtg agcgggcgcc cgatccagcg aatgcggcga agcgccggct
     1441 cttttcccgc acgcttcgcc cgccgcagtt cggatgcaga tcccggagcg gcgggcgtcg
     1501 ccgagtcaca ggccgatcaa ggcgtggaca tcgataatta gccgcgattg gggctggtag
     1561 acgatcgcgt agccgtccat gtatccgcgg acgcatccgg cgcatacagg aggcaatcgc
     1621 tgctcgatga caacgggaaa cgggcggatc cgtctcttcc agcccggagg cagatgcccg
     1681 gtccggtaga gtttcttctc aaggcccgga ggcagcgacc tgggatggta gtactcgcga
     1741 atgatgcgca cgtgctcttc cgtaaagcag gcgtctctat atttttcgtg gtgctcgcga
     1801 tcgaactcta cctctctgcc atgcttgcca tgatccttgg ccacggcggc cgagaacatc
     1861 gttccagcca gcgtgattat caacaacggc gtgaaaaatc gcataggttc gctgcctccc
     1921 taaagagtgt acatggaatt ccgggtttca cagactgcgg actttgactt aaactgcacg
     1981 cggggttcac cgtagaccct tcctatgatc gcaacccgcc tgtctttcca cagattctct
     2041 tgtgcggccg gcgcaatttc tcaatgcggc ccatgcccgg aatcggacga tcttgagcgg
     2101 aaaaatttcc agagtacatt tgcgatcgcc agcgcgggcg ccgccgccaa aaggccgacg
     2161 acgccaaaga gcatgcttcc cgccagtacg gccagaaaaa cagctaaagg gcgtagtccc
     2221 aaccggcggc ccagaatatg aggggtgagg tagtaacctt cgaagccttg aaaaaggacg
     2281 aatgcgccgg ccacggcggc gacgcgccag aactcgcgcc cggcgatcgc cgtcaggcca
     2341 acggccagaa cgaacccgat aaggacgccg aaatttggaa cgaggtgcag aaatccacaa
     2401 agcacggcga tcagcggcca tgccggaacc ttcaacgagc cgaaggcaac agcgtagagc
     2461 gctgacatgg tgagggcgat gatgatctcg ccgcgaatat aagcggcgag ggtccgcgct
     2521 atctcgcgga gcaaatcgcc gaaggatagc ccggcccgca taagtccggc gcgctcggag
     2581 cttcatctcc gcaaagcgcc gcgcatcgac agtctggagt gcgtgcaatt cttgcgccac
     2641 ggccttgctc caatcgagca gccaggggcg gcagccgggt cttccctctg cgaaacatgg
     2701 ctcatccttc ccccaaaagc ttgcccctct caaaatttcc gcgttctgtg ataatgcggg
     2761 taatcagctc cgggggtatt cgatcaaaca gggtaaattc gtcgcgcgac gcaagcaggt
     2821 cggccgtccg gccaggggcg agcttgaagc tatgcccggc ggcgtaaaag ggcttgccga
     2881 agtgggcgca gcagagcgcc aagggcagcg tgccggtttt gtggagaatc gctccatcgg
     2941 catcgaaagc gtcaatgccc accagggcgc aatccacgcg cgccgccgcc tggcccatga
     3001 acgcatccgg aatcagcttc gcgttgaggc tctccgccaa aaggcggccc tcgccccccg
     3061 gcagcgactc cagcaccaaa acggagcttc ctcgtccagc taacgcggct tcgactgtgg
     3121 aactgcggct gatcgtgagg atgcgagcgc ccggcggcaa cagtgcagcg agacgcggcg
     3181 caatgcgctg gttgccctcc agcagggagc gccgcaggcc cggcaggtca gggttctggc
     3241 tttcgagcag tcggcctacg ttcgccaccg tgacgatggc agggtgcatg ctgcggaact
     3301 cacgggcgag ttgaggccag tcttcgggag catccagggc gatgtcaatg gccgagaccg
     3361 ccaggttggt ggcgcccgac actgtatcgg ttcgcaatct ctccagccgc cggagaatat
     3421 cggcgttcac cgccccttgc cgcagggcta ctgcacgagg gctttggcag tagccaggtt
     3481 atcgttggag tcccagacgc gaatggcgat cgtgtgctct cccccggcga cgctgtcggt
     3541 gaggaagtcg taatcgagtt ccttcgaatc aaaaagccgc gtggccggaa gcatcacgcg
     3601 ccactcgccg ccgtccatcg agtattcgga attgtcgatg ttgctgattg catcagccac
     3661 gtgaaagcga acccgcacgc ggccgttctc cacggccgcc gtcaagcccg aaatcgccgg
     3721 cggcgtatta tcaatataaa acggctcgct ttccagcgaa tagatcaacg cctccggacc
     3781 ggggttcgag ggagcgtccg acgcggtcac ccgcaactga tactggccgt ccgcgaatgc
     3841 ggtggaatcc cagcttatat agggctgctc cactttatcg cgcaagagct tccagttctg
     3901 ctcttttaca ccccgaattt ccactttata gaccagctcg tcgttattgt cgtcctcggc
     3961 gagccacctc gcgcccaaat accccttggc ccggttcatg gaacgcggcg cctgcggcgc
     4021 cgcgggcgcg ctgccgcgtt tagtcgacgg acgcgagccc agggccggga gcgagagcgt
     4081 ttgcgtcgag ggagaagcgg cggaaggatc cgcgaatttg taattcggag gcgttatctc
     4141 aagctgcaaa atcgtcggag ctacattctt cggctgatat gcgatctcga cagaatcgag
     4201 caaaggcgag tcgccgctcc gcgtctcgag cacggctttc cactgcacaa accgggcagg
     4261 cgggcagtcc agcggcgcgc cgtccatggc cgtgaccggc ttcgaccagg agctccaata
     4321 ctggtccggg gtgaccaggt ttcccgaacg ggcataaaga gtcaaactgg tgtttggggg
     4381 gacgctgcct gtccacgtca accgtcccca atgcgcgaac atgccggcat cgaacacctc
     4441 cgattcaaac gtacctctgg attccagttc cggcccgagc cggtagatct taccgatatt
     4501 cccagtggct gcgaagacaa cgccgtccgg tccctgtaag aacgcagtca cctgagtcgg
     4561 ggccgccttc accagaaggg aatacacatg gtcagcctca atctggaaaa cgcgtccccg
     4621 gtttcccgtt ccgatcaaca gccggccctt cgggttgaag ccaagcgtat agacaatatc
     4681 ctcggtcgaa ctccacaccc tccgcggctc accatccaga ccgatgcgaa atacctcgct
     4741 gccgccggaa acaccgcttc gcaaagacga tggcagcgaa gcgggttgcg gcgccggcgc
     4801 cgggccggcc ggcgccggcg cgcctcccag caagtttccg accggagcgg gcgccggggc
     4861 cggcgcggcc gggacagcaa gcggcgtcac accaccgctt ccccgcgagc cgacgccggc
     4921 tgcgtacaac atcccatccg gcgccagcgc cagggcggtg atttccttct tcgacgactg
     4981 gtacagcaca aaacccggtc cacccgagcc gggaatccga atcaccagtc ctccaggatc
     5041 ggttccgatt accagatcgt cgtttttatc gatcagcagc gaccgaatat gcgcttcttc
     5101 cgttttgaag aaaaccgtgg ctttgcccga gggacttacg cgaagcaccc tcccctgatc
     5161 gccggtcgcg acaaatagat cgccctgcga attgaaggcc attgcccata tgtacttgac
     5221 cttgggatcc acaaaaagcg acacgcggcc ggaggggtcg attttataaa tcttagagtc
     5281 gggagaagtg gcggcgtaca ggttgtcgcg ccggtctatg gccagcgagt gaatctccaa
     5341 cgcctcggta tcgaaatacg tggtcttctt gccttcggga gtaatcttga aaacccggcc
     5401 gcccggccct ccgccggcaa acaaattccc tttgctgtcg cgggccagcg cccacagata
     5461 cgcaaggggc gcgtcataaa tctcctgaaa tcgcggagcc aatcccagtt tgccgtcgct
     5521 gcgcagcgcg acgtgctcga tcatgccctt ttcgaactcg tcgaaacggg attgctccca
     5581 cgtccgggtg gtctcggcca gagccggaga tgcgagaatc aacgccaata gaaaataaga
     5641 acgcatagat ttgagtccat cgcgaaggtt gcagccaata tacgccgttt tggggcggtt
     5701 tttagagcgg cctaccggac cgtcacgttg agcgaatagc tcccgctcac cacgtaatca
     5761 aacgggatgg cggtctgctc gaccgaagat tcattggcca gcatcaggcg ccggttgcgc
     5821 tcgttgcccg cctcaattac gtttaacacc gaggaaggaa cattgggcag cgtcttatcg
     5881 tcgtagtagg cggtcaccgt cggctgcatc agcgatatgt atacctgatt attgcttaat
     5941 tcctggttga tcaacgacac cgtttcggga aggtccatca gccggttgcc gcccgcggcg
     6001 tacttgttcc ggttaacggt gtccgcatcg ctcaccatca ggcgcagcgt ccccttctgc
     6061 acgccggccg gcaccttgat gcggaattgt ttttcgatcc gctgcccccg gtacggcctc
     6121 aggaacactt tgcccaccag ctcatcgccg ggctccactt cgctcttttc cgtccaggcg
     6181 ttttcgatca ccgccacgcg ccgctcgggc agcaggtcga tggtgacgtc cacgctccgc
     6241 agatgcggca tcttcacgga gttggtgaac agccggttga acttgtcgcc gaaccagcca
     6301 gccagcagca tcggcgccgg aacgggcgta tcgccggagg tgcgcatggt ggagagcgag
     6361 attttctgcc ggccgtccag ttgaatgttg ccgctcagcc ggaacgtgct ctcctcggcg
     6421 aagtcgttca cgccgaacat cgagttatac aacgtgaaca tcatcaactg cggcgtccac
     6481 ttttggttct gaaacacctg aaagtgaaag tccttctccg ctcttactgc gcgcgaatcg
     6541 gcaaacgagc gcacccggac cgataccgga atcgtgggcg cctgctctcc aagcacgccc
     6601 atgatgcccg aatgccgatc ctggcgcagc gctcctacga actcgccgaa attcatgatc
     6661 ttggtgggcg tgaagctcga ggccagggtg tgaaccacct ccgcctgcgc catcggcatt
     6721 tccaccgggc cggtgttgaa catgggatgg ccgaacgcca gaatccgctt gccgtcgttg
     6781 taggtgaccg tgccggaggc ggcgatagac atatcgcccg acaccagcac gcccgaaatg
     6841 gcgtcgcccg gctgtaatgc cgtcgccgca ctgggagcaa tccgcgccgg cgtcccagcc
     6901 gatccgccgc cctgcacagc caccatcccg aggtcgtcga aaattccagc gaagtcgtgc
     6961 agcacagcct cgttgaatcc gccgaaagtc agcggagttt gtattggtgt gaggtatagc
     7021 tcatccgaag ccggccgcag caggccgggg gtgaccgcca cctttttggc ggctgcggcg
     7081 cgttccggcg tgctcgcgcc cgccggccgg ctttggtcga attcgttgat ctccagcatc
     7141 agctcgattg gagtgatgcc gcagatcgca tcgggactga acgctcccag ccgcagcgaa
     7201 acggcgccga tgagtttgcc ttcataatac acagggctgc cgctcatgcc cgccgccacg
     7261 ttggttcgct gcgctttgcc ccccattttg ccgaggatga tatcttgccg cggcccccag
     7321 acgttcttca taatgccgat gatctccacc ggcacaggct ccggctcaga gcccgaaaac
     7381 accgtccagg ccgtcgcttt catgcccggc tttacgtcgg cgagcttcat gatctcaacc
     7441 gctccggctc gcgggcgctg tatttccgcg ccgaatgcgc ttataaggac acacacgcag
     7501 ggaacaagta aggaaaccaa acgcttcatt aaaactccga gcgcttctcg ggcgactttg
     7561 cggggtccca gttcgccctc gaaagctgcc tgtattttag cagaccggcg ggaacgtgcc
     7621 tagaggctgc gccgccccgc cgcgcaaaac gccagaaaaa aggcgggatc cggccattgc
     7681 tgcgccgctg cggtaccaaa actcacattg ccggccatct tgcatgcagg cctctgtctt
     7741 taggcggcgc gatttttcct cgcggaagct gcggtcgaac ctcggctccg cctcacccag
     7801 ctgtaaatgg agacagcgct cgcgccgacg gacagggcga taatcgccag gatgcccccc
     7861 tgtacaactg gtgatttggc cagttccagg attctcgttc caaagaacac ggccagcaat
     7921 cccacggcgg agaatcgtat aaagcggctt atcccgatga ctgtcagcag cttctggcgg
     7981 gggtattgca gcgcggcggc ggcggcaaca aaaggcgtaa agggaaacgg gggcggcatc
     8041 aacgacgcga cagccaaggc ccaggctgcg ctcttcttca cctttttttt cacgtactcc
     8101 agacgccgag gagagacatg cttccccagg ccctcttctc cccctttgcg ggatatcgca
     8161 tcggtcaggg cgcatcccag tacggatccg gccgaagcca taaatgcgta atacgggagc
     8221 atcatgtggc gtctggcggt aagcccgacg atcagaatgt cgttcgccag gggcattacc
     8281 agaaaggaag agtccagaat gcctaagccc agagccccga agccgcccag gtggaacaac
     8341 agggtgtaaa ggtgggccgc aaatcgtttc atcgggaact cttgccagca gcccaatctc
     8401 cacccttctc cggctcagtg gcatcccata caacgaaatc gcacaccgtg gcagatccgg
     8461 caggaggccc tgtcgaattt gacgctgcgg cgggagtgcc gctcgagcca gtcagccgtg
     8521 tggttggccg gcttgagcgc gaccgtatcg ctatgacact tctcgcaagt gaacggcggc
     8581 tcgatttcgc tgtggcagac caggcagtcg gccatctgcg gcatatcggc tttcgataca
     8641 tgagttgcct gctcgagtcc ccgatggcat gccccgcagg ggttctgcgt gttcaggcgg
     8701 gcgcgcatgt cgcccgcctg gccgagatat ttcccggcgt caatagcggc cgcaatggcc
     8761 ggcgccgcat taccgagcgc gaggtggagc ttgtggttga accggagatt cgggaccttc
     8821 gaaagcgtgc tcgacgcggc ggcggcgtgg caatcgatgc agcggacgcc cggtttgcgg
     8881 agcgcttccc gatgagagaa cccgggtgcg cgcgtctgcg cggcgcagaa acacgacgcg
     8941 acggcaatgg tctgaaggct gactgcaatg gcgaggaagt ttcgcagagc cttggttacc
     9001 ccttgtacgt gatatgccag attttgttgc cgccgtcatc ggagaccaga agggagccgt
     9061 cgggcaactg caacagcccg acgggccgtc cccaaacttc ccgccggttc ggcgacaaca
     9121 tccatcccgt gagaaagtcc tgggggccgc cgctcggctt tccgttctgg aacggcacga
     9181 aagcgactga gtagcccgtg cgcttcgaac ggttccagga accgtgaaac gccaaaaacg
     9241 cgccgccctg atattgtttg gggaactgct ggccggtgta aaaaataaaa tcgagcaccg
     9301 cgacgtgcga gccgagcagg acatccggaa cgatcgtctt cttcactagg tccggccgct
     9361 cgcccttgtg ccgggggtct tcgtgcgggc caatgtaagc gtagggccag ccatag
//
LOCUS       DASREK010000194         9100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_94697, whole genome shotgun sequence.
ACCESSION   DASREK010000194 DASREK010000000
VERSION     DASREK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94697"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2481
                     /locus_tag="VFA54_10000"
     CDS             <1..2481
                     /locus_tag="VFA54_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51183.1"
                     /translation="FFFADWQGTRLRIGSTLISTVPSSAQRQGVFSTPIYDPLSTTQD
                     GPGAFHRQPFVNNTIPLNRLDPAAAALLGRYPLPNVLVNGKEAAANNYRRIGVNPEDT
                     DQFDVRLDRYFGERHRLFGRYSYLRDDSAPATPLPDGSGSISSGVIGDTLTRADSVVV
                     DYAYTRSPASVNQLRFGFTRRGFTRSALLTGESASAATGIPNIPSSSFSQTLPIFQIA
                     GFQQIGPPENANSRFTTSVTQFIESYSTLQGRHSLKFGADLRWERLDILQPPDPTGLF
                     SFSPVFTSGLTDSGAPAANTGNALASFLLGQVNSFNIDIQSQFLKPRAHIAEFFVQDD
                     WKAVQRLSVNLGVRYTLNFPSTEANNHGAVFNLQTQQLDFLGQNGFPNTARRLHWGDF
                     GPRISLAFQASDSFVVRSGYGLVWIEQAGITTPFTAPYFPFIQSAGQRSLDNVNPAFV
                     LSQGPSVAILPPNPDSGLGQGVFAVDRNTGSGYAQQWNFTLQKTIGANWSLEAGYLGS
                     KLTHLGVPDTHLNQLPDADLALGTQLTQQVPNPFYGQIPISSSLGAPAIAYQQLLRPY
                     PRFTTVTLFRNNIGNSTYHALQAHLERRFSAGLTLTASYTFAKLIDDASSVFDAAILT
                     GPVANFPVADSYNRHLEKDLSNGDIPHVFTAGFVYELPFGNGRPFPLRGWKNALAGGW
                     NIGGIVKLQSGMPVAVTQITNFNAFAGYGTQRPNRIGNPNSPPGQRSTAQWFNTSAFA
                     MAPPFSIGTSSRNPVRGPGYHDADVMLGKVFSLTERFQAEFHAEAFDVSNTPSFGQPN
                     GVLGNPAFGSITTAFDARVFELALKLHF"
     gene            complement(2716..3036)
                     /locus_tag="VFA54_10005"
     CDS             complement(2716..3036)
                     /locus_tag="VFA54_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51184.1"
                     /translation="MARLPDSAAMAELRLGDIIDDYCSRCKLLTNHSIVSILNGEPAK
                     VYCRTCFFEHNYHHGKGAEKKRSTSRKSKLFDQVLSSITGNPASAGPKESEESPPNEK
                     PKTR"
     gene            complement(3020..4003)
                     /locus_tag="VFA54_10010"
     CDS             complement(3020..4003)
                     /locus_tag="VFA54_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZS51185.1"
                     /translation="MLSWRCMLAVHIERGKVSLRRLRRPPVREGFAVIRMLYGGICNT
                     DLELVRGYYGFRGTPGHEFVGEVVAGSRHLIGKRVVGEINISCRRCAWCRQGLGRHCP
                     RRSVLGIVKHPGAFAEFLTLPESNLHPAPAEVPLPHAVFVEPLAAACELLDQAPVPPG
                     TAVAVLGDGKLGLLAAQVLQVHGAKVHLFGRHREKLRIAEKAGVVGEVRGPKLPTAAY
                     DYVVEATGSHQGLQQAVAMTRPRGTVMLKSTVHRTAPLDTAPIVVNEITVVGSRCGRF
                     EPALALLASGQIHVGEMISDSFPLEKAPNAFLRAGQEGVLKVLLCDNGSSS"
     gene            4291..4725
                     /locus_tag="VFA54_10015"
     CDS             4291..4725
                     /locus_tag="VFA54_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZS51186.1"
                     /translation="MKLATLRAALTAGLWTTVAWLAGPGGATAKALQPVKALKAPYQV
                     GKAAWYGLAFQGRRTASGERFDMNGFTAAHRRLPLGSLVRVTNLANFRSVVVRVNDRG
                     PWKGGRIIDLSYAASKALGLANSGVGEVRLDPVSESLALASY"
     gene            4900..5604
                     /gene="pyrF"
                     /locus_tag="VFA54_10020"
     CDS             4900..5604
                     /gene="pyrF"
                     /locus_tag="VFA54_10020"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZS51187.1"
                     /translation="MRQHPQTARERLIVALDFATSDAALAMADRLRGLAGFFKVGKQL
                     FTAEGPGLVRQLVARGDKVFLDLKFHDIPNTVRSAVKAAASLGVSLIDVHASGGPAMM
                     RAAVEAAAETGITAVAVTVLTSMDQRELAAVGFPDPPERLVLRLAKLALESGLAGVVA
                     APPEVGMLRRELGNDFLIVTPGIRFDASHRQDQQRIGAPEQAVRSGADFIVVGRPITQ
                     AEDPAAVARSILQVLE"
     gene            5617..6606
                     /locus_tag="VFA54_10025"
     CDS             5617..6606
                     /locus_tag="VFA54_10025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS51188.1"
                     /translation="MEPLAVSVVIPVHNEEKNLLPLYDQLIKVLETRPGQFEILLIDD
                     ASTDGSLELLRSLAEVDPRLKVVRLRRNFGQTAALAAGFELARGEVIIAMDGDLQHDP
                     ADIPALLDKIEEGFDIASGWRRERIDNALTRKIPSRIANWMMRHASGVALHDFGTTFK
                     AYRRETIKDIRLYGDLHRFIPALASLNGAHVAEVPIRNVARADGNSHYGLGRTFRVLF
                     DIVTIRFLLRYFTRPMHFFGSVGLVCGGLGAVILTALVADKFLLSHEMMLDHGPLLIA
                     GAMLVLTGIQLFCAGLIGEVLIRTYFESQNRPIYAIREIITQNRSETADERRS"
     gene            6766..7197
                     /locus_tag="VFA54_10030"
     CDS             6766..7197
                     /locus_tag="VFA54_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS51189.1"
                     /translation="MESAELHARLEPERYLVPERERIAGRYREGRQHPPRPPEDVVTL
                     VAELGGEVAGFVDARLEWSFDAMHREMTYCYIAEIAVRAAHRSKGIGAQLLRAAEDWG
                     RRRGAHLASLEYNAANTRAQEFYLERMGYRAASITAVKRLR"
     gene            complement(7403..8242)
                     /locus_tag="VFA54_10035"
     CDS             complement(7403..8242)
                     /locus_tag="VFA54_10035"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZS51190.1"
                     /translation="MLSATIDRLFETKTDTYGPEFHDAFLAFKAALNRGEARAAEPSP
                     ESPLGWKVNAWVKRGILLGFRIGAIVDMSADHGRMPFFDKSTYPLRKFDVSSGVRIVP
                     GGSSIRDGCYIGKGVTCMPPMFINAGAYVDDGTMVDSHALVGSCAQIGKRVHLSAAAQ
                     IGGVLEPVGALPVIIEDDVLVGGNCGVYEGTIVRRRAVLAAGTILTRSTPVYDVVRHT
                     VHRAAGDQPLVIPEEAVVVPGSRAFSSGPGKEWNLSVYTPVIVKYRDAKTDSHIQLED
                     WLR"
     gene            complement(8247..>9100)
                     /gene="dapA"
                     /locus_tag="VFA54_10040"
     CDS             complement(8247..>9100)
                     /gene="dapA"
                     /locus_tag="VFA54_10040"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZS51191.1"
                     /translation="SLDEPALRRLVRRQVEAGIHFLVPCGTTGENPTLTRREHLRVVE
                     ITLEEASGKVPVLAGAGGYNTAEVIELAREIEAIGADGILSVTPYYNKPTPEGLYQHF
                     KAIAAAVRLPIVVYNVPGRTGTNIDPPALVRLSTIDTVIGVKEASGNIAQMAALCHAA
                     PESFDVLSGDDAVTLPLMALGGRGVISVASNEIPAEMARLAALCLEGRFTEAREIHRR
                     YLPLMEVNFIESNPGPVKAALALMGLIEPVYRLPLVGPQPASLARIEKVLESLSLLPA
                     GSFARPA"
BASE COUNT         1721 a         2878 c         2719 g         1782 t
ORIGIN      
        1 tttttcttcg cggactggca gggcacgcgc ctgcgcattg gcagcacact catcagcacc
       61 gtgccttcca gcgcccaacg ccagggcgtc ttctccactc ccatttatga tccgctctcg
      121 acgacgcaag acggcccggg cgctttccat cgccagccgt ttgtgaacaa cacaattccg
      181 ctcaatcgtc tggacccggc ggccgccgcg ctgctcggcc gctatcctct gccgaatgtt
      241 ctggtgaacg gcaaagaggc cgccgccaat aactatcgcc gcatcggcgt caatcccgag
      301 gataccgatc agttcgacgt tcggctcgac cgttattttg gcgagcgcca tcgtcttttc
      361 gggcgttatt cttatctgcg cgacgactcg gccccggcta cgccgctgcc cgacggaagc
      421 ggcagcatct cctccggcgt gattggagat acgcttaccc gtgcggacag cgtagtggtc
      481 gactatgcct acacgcgttc gccggcctcg gtgaaccaat tgcggttcgg gtttacgcgt
      541 cgcggcttca cccggtcggc gctgctgacg ggcgaatcgg cgtcggcggc tacagggatc
      601 ccgaacattc cctcgtcgtc gttcagccag acgctgccga ttttccagat cgccgggttc
      661 cagcaaatcg gcccgcccga aaacgccaat tcccgcttta cgacttctgt tacgcagttc
      721 atcgagagtt actcgaccct ccaagggcgc cacagcctga aattcggagc ggatctgcgc
      781 tgggagcgcc tggacatcct tcagcctccc gatccgaccg gcctgttttc ctttagtccc
      841 gttttcacaa gcggcctgac cgactccggc gctcccgccg ccaacaccgg gaacgccctg
      901 gcgtcttttt tgcttgggca agtcaattcc ttcaatattg acatccaaag ccaattcttg
      961 aagccgcgcg cgcatatcgc cgagttcttt gtgcaagacg actggaaggc tgtgcagcgg
     1021 ctctctgtga atttaggcgt tcgctatacg ctgaactttc catcgaccga ggccaacaac
     1081 cacggcgccg ttttcaattt gcagacccaa cagctcgatt ttctcggcca gaacggcttt
     1141 ccgaataccg cccgccgact gcattggggc gactttggcc cccgcatcag cctggcgttt
     1201 caagcgtcgg actcttttgt cgttcgctcc ggctacggtc tagtctggat cgagcaggcc
     1261 ggcattacca cgcctttcac cgcgccctat tttccgttca tccaaagcgc cggccagcgt
     1321 tcgcttgata acgtcaatcc tgcattcgtg ctctcgcaag gcccgtcggt cgcaatcctg
     1381 ccgcctaacc cggattccgg cctgggccag ggcgtctttg ccgtggatcg gaacacgggc
     1441 tccggctacg cccagcagtg gaatttcacg ttgcagaaga cgatcggcgc gaactggagc
     1501 ctcgaggccg gatatctggg ctcgaaactg acccacctgg gcgtgcccga tacgcatctc
     1561 aatcagttgc ccgacgccga cctggcgctt ggaacgcagt tgacacaaca ggtcccgaac
     1621 ccattctacg gacagattcc aatttcttcc tcgctcggcg ccccggctat cgcctatcaa
     1681 cagttgctgc gcccctaccc ccgcttcaca accgtgacgc tattccgaaa caatatcggc
     1741 aattcgacct atcatgcctt gcaagcccat ctcgagcggc ggttctcggc gggactgacg
     1801 cttacggctt cctacacctt cgccaaactg atcgacgacg cctcttcggt gttcgatgcc
     1861 gccatcctca ccgggccggt ggccaacttt cctgtggcag acagctacaa tcggcatctc
     1921 gaaaaagatc tatcgaacgg cgatattccc catgtcttca ccgcaggctt cgtttacgag
     1981 cttcctttcg gcaatgggcg gccgttccct ctccgaggct ggaaaaacgc tctcgctggc
     2041 ggatggaaca tcggcggcat cgtaaagctg caatcgggca tgccggtcgc agtcacgcag
     2101 attaccaatt tcaacgcttt cgcgggctat ggaacgcagc ggccgaaccg catcggaaat
     2161 ccgaattcgc cgcccggcca gagaagcacg gcgcaatggt tcaacacttc ggcctttgcg
     2221 atggctcccc cattcagtat tggcaccagc tcgcggaacc cggtgcgcgg cccaggctac
     2281 cacgacgccg atgtgatgct aggcaaagtc ttcagcttga cggagcggtt ccaggctgag
     2341 tttcacgccg aagcttttga cgtctccaac acgccttctt ttggccagcc aaacggcgtg
     2401 ctaggcaatc cggccttcgg gagcatcacg acagctttcg acgcccgggt gtttgaactc
     2461 gctctcaaac tgcatttcta aattcggggc gagcggcaaa ggcgagccat gacggtcatc
     2521 ccgggccccg cgagggctct gctgctccgc tcctgcgcgc aaccgccttc acgacccaaa
     2581 agcagagtca tcgctccgct cggaatgacc tgctgtgagc caagcgtcca ctagcgagga
     2641 gcgggcccca acgctggaag gctagcgggt aggcaacgcc gctgaaccaa gggcccggct
     2701 gcgttggcgg gccgcctagc gggttttggg tttttcgttc ggaggcgact cttcgctttc
     2761 ttttgggccg gccgaggcag gattgccggt gatggaagat agaacctgat cgaaaagctt
     2821 gctcttgcgg ctggtagacc gctttttttc cgcacccttg ccgtggtgat agttgtgctc
     2881 gaagaagcat gtccggcaat atactttggc cggttctcca ttcaggattg agacgataga
     2941 gtggttggtg agcagcttac agcggctgca atagtcgtcg atgatgtctc ccaggcgaag
     3001 ttcagccatg gcagccgaat caggaagacg agccattatc gcacaggaga accttcaaga
     3061 caccctcctg cccggcccgc agaaaggcgt ttggagcttt ttccaacgga aaggagtcgg
     3121 aaatcatttc tcccacgtgg atttgtcctg aagcgagcag agccagcgcc ggctcaaatc
     3181 gcccgcagcg ggagccgacc actgtgatct cgtttaccac gataggagcc gtatcgagcg
     3241 gcgccgttcg gtgcacagtg gacttgagca tcaccgtgcc gcgcggccgc gtcatcgcca
     3301 ccgcttgctg gaggccttgg tgcgaaccgg tagcttccac gacgtaatcg taggccgcgg
     3361 tcggaagctt cggtccgcgc acctccccca cgactcctgc cttttccgcg attcgcaact
     3421 tctcgcgatg ccggccgaac aggtggactt tcgctccgtg tacctgaagc acctgggcgg
     3481 ccagcaaacc cagcttgccg tcccctagca cggccactgc cgttcctggc ggaaccgggg
     3541 cctggtccaa cagctcgcac gccgccgcca gcggctctac gaataccgcg tgaggcagcg
     3601 gaacttcagc aggcgccggg tggaggttgc tctccggcaa ggtcaggaat tctgcaaatg
     3661 ccccagggtg cttgacgatt cccagcaccg agcgacgcgg gcagtgccgg cccaaacctt
     3721 gtcggcacca ggcgcagcgc cggcaactga tattgatctc tccgaccacc cgcttcccga
     3781 tgagatgccg tgacccagcc accacctcgc ccacaaattc gtgtcccgga gtgccccgga
     3841 agccgtaata accccgcacc aattccaaat cggtgttgca aatgcctcca tagagcatcc
     3901 ggatcacggc gaagccttcc cgaaccgggg gccgccgtag gcggcgcagg ctgaccttgc
     3961 cgcgttctat gtggaccgcc agcatgcagc gccatgatag cattcgccga gactgctgcc
     4021 gggaagacca ggggtgcgcg cccggcgcaa gcacattaag tataacacta actatatatt
     4081 gtatttattt gtttaatagc cagttataaa attgatatcc cgttaacgcg caagcccctg
     4141 tataataacc cccaatcggc caatgcaagg cgccgctgac ccggcagcga tccgggtggc
     4201 ccagcggagc cgctttttca gttagcgccc ggctatttcg gagcggagta gctggaagcc
     4261 gggcggaatg attcaggagg atccctagtt atgaaactcg cgacgctccg cgccgcgctg
     4321 accgcagggc tttggacaac ggttgcttgg ctggctggac cgggcggggc gactgccaaa
     4381 gctttgcagc cggtaaaggc actgaaggcg ccgtaccaag tagggaaagc cgcctggtat
     4441 ggcttggcgt tccaggggcg ccggaccgcc agtggagagc gctttgacat gaacgggttc
     4501 accgcggcac accgccggct tccgctgggc agcctcgtgc gggtcaccaa tctggcgaat
     4561 tttcggtcgg tcgtcgtgcg ggtcaatgat cgaggaccgt ggaagggcgg ccgcatcatc
     4621 gacctgagct acgcggcctc aaaggccttg ggcctggcaa attccggtgt gggcgaagtc
     4681 cggctggacc cggtctccga atccctggcg ctggcttcgt actaattatc tccatcgtct
     4741 tcttttcggc tttccgggga aactgccacg ggcgagcgga ggtgttccaa agcgcggcag
     4801 gggaaggctc aaagcgcccg gtgcaagctc cgccgaaaag gcccgggccg gcgggcgcct
     4861 ttagcggccg ccgggccgat cgtgctacaa ccctaagacg tgcgccaaca tcctcaaacc
     4921 gccagagaac gactgatcgt cgcgctcgat ttcgctacca gcgacgctgc gctcgccatg
     4981 gctgaccggt tgcggggctt ggcggggttc ttcaaggtcg gcaagcagct tttcacggcg
     5041 gaaggcccgg gtcttgtccg ccaacttgtt gcgcgcggcg acaaagtttt tctcgacttg
     5101 aaatttcacg acatcccgaa caccgtgcgg agtgcggtga aagccgccgc cagcctgggc
     5161 gtgagcctga ttgacgtgca cgcctccggc ggccctgcca tgatgcgcgc cgccgtggag
     5221 gcggccgccg aaacgggaat caccgcagtg gctgtgacgg tgctgaccag tatggatcag
     5281 cgggaactgg cggcggtggg gtttccggat ccgcccgagc ggctcgtatt acgtctcgcc
     5341 aagctggcgc tcgaatcggg attggctggc gtggttgctg cgcccccgga agtcggaatg
     5401 ctccgccggg aactgggaaa tgatttcctc attgttactc caggcattcg ctttgatgca
     5461 agccatcgcc aggaccagca acgaatcggc gcgcccgagc aggcggtgcg cagcggcgcc
     5521 gatttcattg tggtaggacg gccgatcacg caggccgagg atccggcggc agtagcccgg
     5581 agcatcctgc aagtgctaga atgatgcgtc acttccatgg agccgttagc cgtttccgtc
     5641 gtcattccag ttcacaacga agagaagaat cttctcccac tttacgatca gttgataaag
     5701 gtcctcgaga cccggcccgg gcagttcgaa atcctgctca tcgacgacgc ttcgaccgac
     5761 ggcagcctgg agctgctccg cagcctggcc gaggtggatc cccgcctgaa ggttgtccgg
     5821 ctgcgccgca actttgggca aaccgccgcg ttagctgcgg gcttcgagct ggcgcgcggc
     5881 gaggtgatca ttgccatgga tggcgacttg cagcacgacc cggccgatat tcctgcgctg
     5941 ctcgacaaga tcgaggaagg cttcgatatc gccagcggct ggcgccgcga gcggatcgac
     6001 aatgccctta cgcgcaaaat tccctctcgg attgcgaatt ggatgatgcg gcatgcgtcg
     6061 ggcgtggctc tgcacgattt cggcaccaca ttcaaagcgt accggcgcga aaccatcaag
     6121 gacattcggc tctacggtga tttgcaccgc tttatccccg ctctggccag cctgaacgga
     6181 gctcacgtgg ccgaagtgcc gatccgaaac gtggcgcggg ccgacggcaa ctcccattac
     6241 ggcctggggc gcacgtttcg cgtgctgttc gatattgtca ccatacggtt tttgctccgt
     6301 tacttcacgc gcccgatgca cttcttcggc tcggtaggac tggtttgcgg cgggctggga
     6361 gcagtgattc tgaccgcgct cgtagcggac aagtttttac tcagccatga aatgatgctg
     6421 gaccatggtc cgctgctcat agccggggcg atgctggtgc tgaccggcat tcagcttttc
     6481 tgcgccgggc tgatcggcga agtgctgatt cggacctact tcgagagcca aaaccggccc
     6541 atttacgcca ttcgggaaat catcacgcag aatcgcagcg aaaccgccga tgagcgccgg
     6601 tcctgaggcc ggcacgcggc agggcggctc cggtaccact tctagcgtgc gcgcccacgc
     6661 aaagcgctcc cgcggtatgc tcggtgtatg actcccgccg gtcctgccgg tgtgatgatc
     6721 cgggccgcga gaccggagga tgctgacggt atcgccgcgg tgtttatgga aagcgccgaa
     6781 ctccatgcgc gactcgagcc ggaacgctac cttgtgccgg agcgcgaacg aatcgctggt
     6841 cgttatcgcg agggccggca gcatccgccc cggccgcccg aagatgttgt aacgctcgtc
     6901 gccgaactgg gcggcgaggt tgctggtttc gtcgacgccc gccttgaatg gtcatttgac
     6961 gccatgcacc gcgagatgac ttactgctac atcgcagaaa tcgcggtaag ggccgcgcac
     7021 cggagcaaag gcatcggggc acagcttctc cgggcagcgg aagactgggg gcggcggagg
     7081 ggcgcccacc tggcctcatt ggaatacaat gcggcgaata ccagagccca agaattctac
     7141 ctggaacgaa tgggatatcg tgcggcatcc atcactgctg taaagcgctt gcggtgagca
     7201 tcgcgatttg ctggaatgtg cgctatgttt gcagggagat gggccggctc gaagcgcctc
     7261 tcgctggcgt taaaattttg acacgaggtc ctcaaccgcg atccgctttg cggcgcgctg
     7321 gggaccgtct agggcggcga aaatcatgca gcgctgggtt tctcttacct ggccccgcct
     7381 cttgtgttga gttcccggcc gcttagcgca gccagtcttc caactgaatg tgggagtcgg
     7441 tcttggcgtc gcggtatttg acgataaccg gcgtataaac agagagattc cattccttgc
     7501 cgggaccgga agaaaaggcc cgagatccgg gaaccacaac cgcctcttcg ggtatgacca
     7561 gcggctgatc cccggctgcc cggtgaacgg tatggcgcac gacgtcataa accggagtcg
     7621 agcgcgtaag gatggttccg gcggcgagca cagcgcgccg gcggacgatt gtcccttcgt
     7681 aaacgccgca gttgcccccc acaagcacgt catcctcaat gatgaccggc agcgcgccca
     7741 ccggctccag cacaccgcct atctgcgccg cggcgctcaa gtgtacgcgc ttgccgatct
     7801 gcgcgcaact gccgaccagg gcgtgcgagt caaccatcgt tccatcatcc acgtaagctc
     7861 cggcgttgat aaacatcggc ggcatacagg tgactccctt tccgatataa cagccgtcgc
     7921 ggatcgagga gccgccaggc acgatgcgga cgccgctcga aacgtcgaat ttgcgcagcg
     7981 ggtatgtcga tttatcgaag aagggcatcc ggccgtggtc ggcggacata tcgacaatgg
     8041 ccccgatgcg gaatcccagc aggatgccgc gcttgaccca cgcattgact ttccagccca
     8101 gcggtgattc cggcgagggt tcggcagcgc gcgcctcacc ccggttcaga gcagccttga
     8161 acgcaagaaa cgcgtcgtga aactcgggac cgtaggtgtc tgttttggtc tcgaacaacc
     8221 ggtcgatcgt ggcggaaagc attgcgctaa gcgggacgcg cgaagctgcc ggccggaaga
     8281 aggctcagcg attcgagcac cttttcaatc cgggccaggc tggccggctg cggaccaacc
     8341 aatggcagcc gataaaccgg ctcaatcaaa cccatcaatg ccagcgcggc cttgacgggc
     8401 ccagggttcg attcaatgaa attcacttcc atgagcggca gatagcgccg gtgaatttcg
     8461 cgggcctcag tgaaacggcc ctccagacac aacgcggcca gccgcgccat ttccgcggga
     8521 atttcattcg aggccacgga tatgaccccc cggccgccca gcgccatcag cggcaaggtg
     8581 acggcatcgt cgccagagag gacgtcaaag ctctccggcg cggcatggca gagcgcggcc
     8641 atttgggcaa tgtttccaga cgcctctttg accccgatca cggtgtcgat ggtgctgagg
     8701 cggaccagcg cgggggggtc gatattcgtg ccggtccggc cgggaacgtt gtagaccaca
     8761 atcggcagcc gcacggcggc ggcgatggct ttgaagtgct gatacaagcc ttccggcgtg
     8821 ggcttgttgt aatacggggt cactgaaagg atcccgtccg ccccgatcgc ctcgatttcc
     8881 cgggccagtt cgatcacctc agccgtgttg tatcctccgg ctccggcgag cacaggaacc
     8941 ttgccggaag cctcttccaa cgtgatctcg acgacgcgca gatgctcgcg ccgcgtaagg
     9001 gtgggatttt cgccggtggt cccgcaggga accaggaaat ggattcccgc ctccacctgg
     9061 cggcgcacca aacgccggag ggccggctca tccaggctca
//
LOCUS       DASREK010000195        30481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_94790, whole genome shotgun sequence.
ACCESSION   DASREK010000195 DASREK010000000
VERSION     DASREK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 30481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30481
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94790"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFA54_10045"
     CDS             <1..773
                     /locus_tag="VFA54_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HZS51192.1"
                     /translation="RQYHQNDITFSLLYSFTENFLLPISHDEVTHGKRSLISKMPGDQ
                     WQQFANVRAFLAYMYGHPGKKLLFMGTEIGQWNEWNHDKPVEWELLQFDAHRSLQRYV
                     ADWNRLYRRERSLWEVDFDWRGFEWVDFHDAQNSIIVFIRRPAPAISGQPSPAERSAP
                     EQMAAAGSAEAALSGEAPEFLLFACNFTPVVRQGYRIGVPEPGWYQEILNSDSKLYGG
                     SDAGNAGGVSTEEIPSHNRPYSIAITLPPLAVVVFKKT"
     gene            complement(987..2045)
                     /locus_tag="VFA54_10050"
     CDS             complement(987..2045)
                     /locus_tag="VFA54_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS51193.1"
                     /translation="MSAGLPARKPASSSSFMNVGLIGTGAIAHKHAEAYRAAGYRLVA
                     VNNRSVEKGRQFAEKWGAEFVADYRDLCRRRDIDYVDVCTFPDFRLQPVEECARTGKH
                     VLVQKPIATSLATARQMIETARAADIRLGVISQHRFDPGAIFVHKALAAGRLGRLLEA
                     DCYVKWHRPQAYYDRPGKGSWQVEGGGALINQAIHCVDLIRWFGGPVAEIYGHWQLGA
                     AHKMESEDIANAVIRFASGATGVIQGSTAFFPGYPERIELHGTKGSAILTGDRLTAWD
                     VAGDKMEDVPLATKLASGAADPMAISIEPIKAQFLDFGDAIRNRRKPLVAGEEGYAAL
                     EIILGIYESARRGVKVTF"
     gene            complement(2135..2656)
                     /gene="purE"
                     /locus_tag="VFA54_10055"
     CDS             complement(2135..2656)
                     /gene="purE"
                     /locus_tag="VFA54_10055"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZS51194.1"
                     /translation="MSDSKNHKPLVGIVMGSDSDLPLMAETAKTLKQFSIPFEIEITS
                     AHRSPARTAEYARTARKRGLKAIVVGAGGASHLAGVIAAESTLPVIGVPIANSPLSGM
                     DSLLSTVQMPGGVPVATMAVGKAGAVNAAIYAAQIIGTSDPHVEGVLAKFKEDLARGV
                     AEKSEKVKREFQV"
     gene            complement(2653..3930)
                     /gene="purD"
                     /locus_tag="VFA54_10060"
     CDS             complement(2653..3930)
                     /gene="purD"
                     /locus_tag="VFA54_10060"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZS51195.1"
                     /translation="MKILVIGSGGREHAIVWKLRQSPLADEVYCVPGNAGISSEAICR
                     GGDIVSPRLMADLAAEIGADLTVVGPEAPLVAGIADEFRSRNLRIVGPDAQAARLEGS
                     KVFAKQFLRDSGVPTADFLVLESPEDVARNIGAFGFPVVLKADGLAAGKGVLILDKES
                     EARAAAELMLAGKIAGPAGRRAVLERFLPGEEVSFIVLSDGAGYYALPSARDHKAVYD
                     GDRGPNTGGMGAYSDGAILSEAEKQGILQRIVEPTLDRLYKAGCPYRGFLYFGLMMTA
                     EGPQVLEFNVRLGDPEAQALLFRLESDLVPLLQCAAQGAGITDVAVKWSSGPAVCVVL
                     ASAGYPGHYEQGYEIFGIDEAEELGAKVFHAGTKFVEGRLVTAGGRVLGVTAGGEDLP
                     AAIDNAYAAVAKIRFEGMHYRRDIGVKGLARLQ"
     gene            4007..5935
                     /gene="mnmG"
                     /locus_tag="VFA54_10065"
     CDS             4007..5935
                     /gene="mnmG"
                     /locus_tag="VFA54_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZS51196.1"
                     /translation="MALSFHEKYDVVVVGAGHAGCEASVATARMGLRTALVTLNLDLI
                     AQMSCNPAVGGIAKGHLVREVDALGGIMGQVTDAVGIQFRLLNTSRGPAVWSPRAQCD
                     KKQYRIEMKRRLEAERNLVIKQAEVVDLVLEEEAGPTFDGLPARRVRGVALRDGRLIS
                     AGAVVITTGTFLNGLIHCGEQTYPAGRSGEPPSVMLGERLRNLGLRCGRLKTGTPPRL
                     DARSIDWSRFEIQPGDPEPTPFSFRTDKIEREQVVCHIAFTTEETHRVLRDNLHRSPL
                     YSGQITGIGPRYCPSIEDKIVKFPDKPRHQIFLEPEGLDTNEIYVNGMSTSMPIDVQT
                     AMVHSVPGLENAEMIRPGYAIEYDSVDATELAPTLEVKKIRGLFLAGQINGTSGYEEA
                     ACQGIMAGINAGCAVKGLPPVTLDRTQGYTGILIDDLISKGTNEPYRMFTSRAEFRLH
                     LRIDNADQRLTPIGRQVGLIDDEAWQKFCAKQERMARFRQFLNVYRPDPRDHAADRLL
                     RKVNQSAGDCPVASQLLKRPEIVIEDLAPVLQEKLGLPLKRLELKAIETEIKYEGYLA
                     QQQKHIEQLKKAESRRIPADFPFRGLPGLSREVVEKLERVRPVTLGQAGRIPGVTPAA
                     VSIINVYLDLPARQGARN"
     gene            5942..7090
                     /locus_tag="VFA54_10070"
     CDS             5942..7090
                     /locus_tag="VFA54_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51197.1"
                     /translation="MDESLACEIRPGGGWDLSPVPNVPGVFAVWPSQGPPYIGKSSLL
                     RRRLARLLRPAETQSRLLNLAQIACRVEYQPAGSPFESMVLLYKLARRHRPGDYQKYL
                     KLRIPPFLKINLSNAYPRCYVTHKLGQRRAFYFGPFPSRAAAERFQNAFLDLFQMRRC
                     VEDLEPTPTHPGCIYGEMKMCLRPCQAAVTAAEYRSETERVVQFLATNGESLLKQLRD
                     ERDRASAALQFEQAARIHKKLERVAEALQLKDDLAGDLDRLHAIIIQRSVDPCAVELW
                     LLYQGFLQPQRRLHVAAEAGQPLSLDSKLRETLSAVSWETGAARQRAEHLALVARWYA
                     SSWRQGEILRFEDFDHLPYRKLVKAISRVAAGSTQPAGARISGSYRPG"
     gene            complement(7087..7803)
                     /locus_tag="VFA54_10075"
     CDS             complement(7087..7803)
                     /locus_tag="VFA54_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS51198.1"
                     /translation="MLHSGCSADGRPSKVESGLANIAKEVVIPLEAKERKNPLPVNGA
                     AVKKGMDLYRRSCALCHGSDGRSNTTLGRDLYPPAIDLTSPHVKRWDDAELFWIIQNG
                     VRFTGMPAWKDVFDDTQTWEIVLAIRELQRAKPQPETALQAASGGQDLVQEGGLLFRQ
                     ENCIGCHRLDGDGGGVGPDLSDEGNRGRTDAWLIGHFQNPSAYVRGSIMPPATNLNQR
                     QLQALTAFLQQQKKTPARHR"
     gene            8092..8688
                     /locus_tag="VFA54_10080"
     CDS             8092..8688
                     /locus_tag="VFA54_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51199.1"
                     /translation="MHEDAEAENQTSSDRGARRAVEWTANLLLGAGSGGLMGLLIAGV
                     IGIRHGPDGAPLAVTLLLTAGLAAIMAFLSVRQEPPARPVAPRVAWGVLLTGAALVIA
                     LAVYLFQGAYIQSVRGSGGRAEPPLPIWPVSRAALGASLVSLLAGLAAALLGWAELAA
                     NRERYGGGKWVALSVLAAGAWLALALACYVIGYGFTFI"
     gene            complement(8667..11216)
                     /locus_tag="VFA54_10085"
     CDS             complement(8667..11216)
                     /locus_tag="VFA54_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 metallopeptidase family protein"
                     /protein_id="HZS51200.1"
                     /translation="MFRQGLRLVFWLLFIQIWTPAARAAVPSPESQFGHRMGADRTVL
                     EWSKVVEYFGLLERSSDRIRVQELGKTTEGRPFILAIISDPETLRSLDRYRQIQAKLA
                     DPRLTNLAEAEQLIAAGKTVVLITCSIHSTEIASTHTAVEFAYKLLAEETPRHRSILA
                     NTILLLVPSLNPDGVDLVTRWYRRQLGTPWEGTPPPGLYQKYVGHDNNRDWYMFTQVE
                     TRLAVEKIHNVWHPQIVYDVHQMGPYAARLFVPPWLDPVDPNVDAVIAQEMNMIGTGI
                     AADLTAAGKTGVAINAMYDFWTPSRHYQAYHGGLRILTESASARLATPIHVAPDQLDT
                     GALGYNARERSWNYLQPWPGGEWRLRDIVDYQLIAFESCLYQAALRREDLLRNFYGVG
                     LRALERKDPPYAFIIPRRQHDPNAMTRMLETLQFGQVEIERARVAFRAGGGEYDAGDY
                     VIRLAQPYGSYAKTLLERQRYPDLRIYPGGPPRRPYDVAAQTLPLLMGVRAETIESPF
                     HVVLDRVEKIEPEPGRVDGSSVAALSPALTNAWIAVNRLLKAGAAVWRDLKTGAFLID
                     NNGPAKSLLPGLARDLGLPFTAARSRSGSARLRLPRIGLYQSYVPNTDEGWTRWLLEQ
                     FEFPYTTLFNRDIRAGRLLARFDVIVLPDATPQSIESGFSPAPDRGSTSVMPPEFSGG
                     LGDPGAEALGEFVRGGGVLLALNRSSLYAIERLGAGARNVLAGVPNREFYAPGSLLAA
                     DVDTRHPLAFGMENPTAVWCESGPAFEPAPASSNPPAKSVVEYPRRNPLLSGWLLGPE
                     LVENRSAVMDAPAGRGHIILFGIRPQYRGQSYATYKLFFNGLFLAQMNVKP"
     gene            complement(11371..12153)
                     /locus_tag="VFA54_10090"
     CDS             complement(11371..12153)
                     /locus_tag="VFA54_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51201.1"
                     /translation="MRKLLTVVLLFVFTAVGRSADVATAEANLRRVQMLVEAGAAPRS
                     ALKAAEEQLEDAWHEQILQETLYRGDVTPQEVPRMLGAASALLRRAQNALAEQQKLVK
                     EGVLAPKALERAQKEEAQAEKQWQLAESRAKLTQELAEMARRESEMAEQAKADLALAA
                     KFEGKGTVTTGDLARLESAFYQKFSHPLPVSAWGETAVHRSLGFDHRDRVDVALNPDQ
                     PEGRWLMRMLDRLSVPYIAFRHAVLGSATGAHIHIGLPSPRL"
     gene            12242..12583
                     /gene="rsfS"
                     /locus_tag="VFA54_10095"
     CDS             12242..12583
                     /gene="rsfS"
                     /locus_tag="VFA54_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZS51202.1"
                     /translation="MDFWQAAVEAARSKKAEDISVLDLHEVASFTDTFVICSGMSARQ
                     NQAISDEVERKLKEQGLRPASIEGYQQAEWILMDYGDFIIHIFSPRARNYYELERLWK
                     NARRIAIERAV"
     gene            12583..13092
                     /locus_tag="VFA54_10100"
     CDS             12583..13092
                     /locus_tag="VFA54_10100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS51203.1"
                     /translation="MMPPAPSTVGFETRPATAADAPVLWRIFNQAIEDRLATFAARAC
                     PLDEVEGWFRGKRFHALLAENFRQPVGWASLRPYREGPAFSTIAEMSVYVDRTWRRHA
                     VGQLLAAALIEEARSLGLHKLIGYLLESNISSRKLVEKLGFRMVGVHRRHGAPAAGCP
                     DVIVVEKLL"
     gene            13120..13656
                     /gene="cobO"
                     /locus_tag="VFA54_10105"
     CDS             13120..13656
                     /gene="cobO"
                     /locus_tag="VFA54_10105"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZS51204.1"
                     /translation="MAETKGLLMVYTGNGKGKTSAAMGSGFRAIGQGLKVLMVQFIKG
                     SWHYGELDAAKMLPEGLFEIRVMGRGFVKIGAEKPDPADVEMVEKAWGMVVEEMHAGR
                     FDMIICDEINYAIHYGMIDIDRVVSDLRSRPETVHVICTGRNAHAKLIGAADLVSEIK
                     QIKHPYEKGILAQRGIEY"
     gene            13659..14504
                     /gene="accD"
                     /locus_tag="VFA54_10110"
     CDS             13659..14504
                     /gene="accD"
                     /locus_tag="VFA54_10110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZS51205.1"
                     /translation="MPWFKKDKAPEPSEGKKVRTEGLWIKCDGCRQIIWKRDLETNLN
                     VCPKCGHHFRVDARTRLAQLLDEAKWTELDADLASSDPLSFADQKPYRERLSAMKAAT
                     GLNDALITAEGSLAGRPVIVCAMEYRFIGGSMGCVVGEKITRAIERAIETRRPLLIVS
                     ASGGARMMEGAVSLMQMAKISCALMKLEEARVPYISILTDPTTGGVTASFAMLGDFNI
                     AEPNALIGFAGPRVIEQTMRQKLPAGFQRSEFLLQHGMLDAVVKRGELKAWIARALDF
                     LCGPV"
     gene            14602..15906
                     /locus_tag="VFA54_10115"
     CDS             14602..15906
                     /locus_tag="VFA54_10115"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZS51206.1"
                     /translation="MTYPEAVQRLLRLGNEVKTAKLSLDRMDALLEKLERPERAFRSV
                     HIAGTNGKGSTAAMIEAGLRAAGRRTGLYTSPHLVRINERIQVNGEEISDADFCSVFE
                     TVERAVEQLLAQGSLELHPTYFECLTAMAFCHFRSAGVELAVVEVGLGGKLDATNVLL
                     PSVSVITPIDFDHEAFLGKGTASISAEKAGILKPGVPAVFAPQSPEAEEVLEARAREL
                     QICVVRAGRDWKAGRISHHHGYYEFEAVSDTGRLIDARLSLAGEHQITNALAAIAALD
                     LLGLSPGAIERGLRVARWPGRLEQAAEKPTVLLDGAHNPAGARALARFVRQHCPGRRV
                     WLIYAAMRDKAVDEVAGILFPLAHKVFVTGLAQPRAVSPEALAALVAHHHVCIQTTAN
                     VAEALAAARALASEADVILATGSLFLVGEAKALLASRARSAA"
     gene            15909..16661
                     /locus_tag="VFA54_10120"
     CDS             15909..16661
                     /locus_tag="VFA54_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZS51207.1"
                     /translation="MLRALLWTDPAICILTVFMGSISLLASIVDGTGHMQHRIARRWA
                     RMVMAVSGATVRVTGLDQLTPGAAYVFCSNHLSLIDTPLVFGYLPWEFRVLAKKIYFG
                     VPFLGWHLRRAGHLPVEPDNVRASVRNVAEAARQAALGLSIVIFPEGGRSRTGALGEF
                     HAGAAYIAIKAGAPVVPMGIVGTREVLPPGSIIVRPRPVELRIGAPIPTAGLTTRHAK
                     DLLLELRRRIVELSGEPEPSARKEMPATKHSS"
     gene            16765..18390
                     /locus_tag="VFA54_10125"
     CDS             16765..18390
                     /locus_tag="VFA54_10125"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZS51208.1"
                     /translation="MYTPEAASFHPQSGLTAPSARERAARALHMRHLVEQIRQIESRL
                     RAGGGPARVERQHRAGKLTARERLEKLLDPGSLFLEVGLLVASDKYDGQAPAAGVVTG
                     VGLIAERPVVVVANDATVKAGAWWPETITKILRCQEIAMRRRIPIVYLVDSAGVNLPY
                     QDGIFPGQYGAGRIFYYNSRMRRELKVPQIAAVMGPCIAGGAYLPALSDVIIMVEKTS
                     FMGLGGPNLVKGATGQTVDAESLGGAEMHNTLSGVAHYRAANDAECLALIRQLIDELP
                     RRPASPAHEPSPPLLNEDEIYETLPADHRLPYDIQEIVFRVFDQQGYLEFMADFAPEV
                     LCANARVMGFSVGLIANRRGFFPSGGKPRIGGIIYTESARKVSYFVENCSRLGFPLVY
                     FQDVSGFMVGPEAEQSGIIRAGAEMVETMSCAGVPKIIVTLNHASGAGYYAMAGQGFD
                     PDFTFSWPTARVGVMEGESAVQALFAAEIEKVQASGQAMPEDLREAIERTRADYERWL
                     DAKFAAARGHVDAIIDPRETRRVLELALRVTSG"
     gene            complement(18421..18849)
                     /locus_tag="VFA54_10130"
     CDS             complement(18421..18849)
                     /locus_tag="VFA54_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZS51209.1"
                     /translation="MSGRVEVGRFNFRFECQKGCTNCCTQKGHIFLTEEDISRIAAYL
                     ELDPKAFEKRYVCRGKYGPRLRLPRSRSCHFLVEGGCSIHDVKPIQCRTFPYWPENVA
                     SRSAWKKLRTYCPGIGVGPLVQIQAVREQAQAYQDAFPGL"
     gene            complement(18846..19103)
                     /locus_tag="VFA54_10135"
     CDS             complement(18846..19103)
                     /locus_tag="VFA54_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51210.1"
                     /translation="MFARKFRLSLAEGEQSRPVPKQWMDQFFMRNFIGLSEFDETLVT
                     GDGELEAGFAVTAEQIQEHFEKWLRGRKLIRQDAVLEVRTQ"
     gene            complement(19090..19584)
                     /locus_tag="VFA54_10140"
     CDS             complement(19090..19584)
                     /locus_tag="VFA54_10140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZS51211.1"
                     /translation="MTLRDYCPDDLEALCAIDRACFDPAVAYTRKEMAALLGRRAAVA
                     IVAEAAPGRLAGFVAAYKQPNRTAHIITLDVLPAWRGRGLGRRLMLCCEERLRAAGVR
                     KVHLETAVGNRSAQALYRSLGYTRIRCLRRYYATGENAWAMEKTLAAKPAKEPPEERQ
                     LVCA"
     gene            19644..20132
                     /locus_tag="VFA54_10145"
     CDS             19644..20132
                     /locus_tag="VFA54_10145"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZS51212.1"
                     /translation="MSPGVIEKRMRLFGDRCAELGLAATHQRRAIYRALAESEQHPQP
                     EAIYRRVRRLIPTISLATVYKNIKTFVEMGLLREVGCEAGSYRLDANLDRHHHLVCVN
                     CKSVVDFYDRKLDQVRAGQTSPGNFRIFRYQVEALGLCPACRSKQGLRKNKKRGQYGQ
                     SR"
     gene            20116..20682
                     /gene="dps"
                     /locus_tag="VFA54_10150"
     CDS             20116..20682
                     /gene="dps"
                     /locus_tag="VFA54_10150"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HZS51213.1"
                     /translation="MGKVARQVVEQAGVNVDVLLDKLVRAAGAEFTTFYYYTILRVNS
                     IGFDGEGLKEIIEDARIEDRNHFEALTPRIYELGGALPRDIRGFADVAGCPDAYLPAD
                     PRDMKAMLNVLVEAERCAIRTYTEICNMTFGKDHRTYDLALAILHEEVEHEAWFSEYL
                     NEGPSGHFRRGAPGASPYVRRFMTADFS"
     gene            20784..21128
                     /locus_tag="VFA54_10155"
     CDS             20784..21128
                     /locus_tag="VFA54_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HZS51214.1"
                     /translation="MPFQKLARAEEIPPGRTKFLWAGEFPVILANLAGRIYALYGLCP
                     HRENPLEGATLWDHLLDCPWHHFQYDVRTGENYFPKNVYPADYPRLQEQLRPLRTFPV
                     EIKDGDVWVNLE"
     gene            21110..21460
                     /locus_tag="VFA54_10160"
     CDS             21110..21460
                     /locus_tag="VFA54_10160"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZS51215.1"
                     /translation="MGESGVSSLAAKHCVPCRGGVPPLKGEELRKLAAQVADWKVVDE
                     HHITKTFLFPDFKTALDFVNRVGAVAEQEGHHPDLTLTWGRVDVKTYTHKIDGLTESD
                     FILAAKIDQLFFNK"
     gene            21773..23422
                     /locus_tag="VFA54_10165"
     CDS             21773..23422
                     /locus_tag="VFA54_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51216.1"
                     /translation="MTMQPGTNTIESFKALKGGDGLRIREDLPRLIRDGWKSMSRADK
                     DLLKWVGVFFRNPTPGRFMMRIRMPNGFAASEQLRAIAELSRRLGNNILDITTRQQVE
                     LRGFTIESVPEIWEKLRGVDLHSLQTGMDNVRNINGCPLAGLTPGELFDASPVLLELD
                     RILVGKDGNPEFTNLPRKFNVTITGCLENCTHSESQDIALIPARKGGRAGFNVLAGGK
                     MGSGGFTIATPLGVFVEPGEAAQVCAELIRIYRDHGPRDARSKCRLAFLLEQWGATRL
                     RAELEYRLKRGLESAGEDARRAGHTDHLGVTRQKQAGLVAVGLCVPTGRLDADQMSEL
                     ARLAEAYGNGQIRFTTGQNAIIPNVPAHRTAALFREPLLEQFSPEPSPFFRGMVACTG
                     TDFCNLAQIETKQKAVELSRALADRLGDDIAPLTIHWSGCPAACGNHQAADIGLRGMK
                     INAGGKTIDAVAIYTGGRTGPEAAAGEQILDAAPLDANLADTIAAIIRQAGLARPLKH
                     KAASRNAALAPAAPQFVPLQSIGNQQGAEARAVSSGVSRQV"
     gene            23537..24391
                     /locus_tag="VFA54_10170"
     CDS             23537..24391
                     /locus_tag="VFA54_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HZS51217.1"
                     /translation="MDYVNPKELMTEALDVAAKKAALPAGDLLLRGALAGGLLGYATS
                     LVVVIQTQGLPGIVGAALFPVGFAILVLLGLELATGNFALLPAGLMAGRLPLRALLRN
                     WLWVYAGNLAGSILYALLFYLAVTNCGASSAGPLGDQLRQMAQKKTLGYMALGASGWS
                     TAFIKGVLCNWMVTLGAVLALASRSTIGKIVAMWLPIMTFFAHGYEHSIVNMFVMPAG
                     MLFGAPVSVSSWWLWNQIPVTMGNIFAGALFTGLALYATYAAKLPSAEAPLNLPEREA
                     AFASTLRG"
     gene            24397..25749
                     /locus_tag="VFA54_10175"
     CDS             24397..25749
                     /locus_tag="VFA54_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZS51218.1"
                     /translation="MRAQCFPLALAAAVFLARGLSAQQTAAPSPAGSSPVSTSLVPSD
                     ELNKELPKWLRFSGEYRGRAEGFTGAGFKPDVDDDYWLSRLRLNMKIQPTDWLKFGFQ
                     AQDARVFGKDQSPAAPPYQNTMDLRLAYIEIGDAEKKSFGLRVGRQELAFGEERLIGN
                     VNWVNTARSFDAVRATLRRGKYRLDAFASSVVVAKDGSFDHSQAGSDLHGLYGGMENV
                     VPGSTIEPYVFWRLARAVVSEEGTPSHLNFKTVGFRWVGKLPLHFDYGAEVAGQIGSL
                     GADNVGAWAGHWLAGYTLANLRYKPRLVAEYNFASGDKNPRDGARGTFDQLYPTGHDK
                     LGLADQVGWRNIHHVRAGVEFRPQAKLLVIGKYNAYWLDSARDALYNAAGAAVARVAD
                     GSAGKYVGQEADFQSVYTFSRQLQFGGGYARMAPGTFLKKATPGASYNYPYMLFTYLF
                     "
     gene            26107..26928
                     /locus_tag="VFA54_10180"
     CDS             26107..26928
                     /locus_tag="VFA54_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HZS51219.1"
                     /translation="MRQGYLIDMDGVIYRGSEPIPGAADFILYLQEQGIPYLFLTNNS
                     AYTPVDVAVKLRKFGIETSPEHVYTSALATAEFVHRQKPNGTAFVIGEGGLLSALNDV
                     RYAISRESPDYVIVGEGRVLNFELAEQAHRLIAAGAQLISTNADTWCPTDSGPRPGCG
                     AIVALLEAATGRKAYHVGKPNPFMMRTARKLIGLRTDEVVMIGDTMETDIKGAAELGF
                     RSILVLTGSSTRESLRDHPFSPTQVVESVAALVPEYIMGERRVGEHRNGSAFRYG"
     gene            complement(26919..27203)
                     /locus_tag="VFA54_10185"
     CDS             complement(26919..27203)
                     /locus_tag="VFA54_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51220.1"
                     /translation="MHIEAAGRNRIDVIGEPQELLMAVTPAAIADRDSRRHGQRREQG
                     RNSVPFVAKPAVYEGFEKTVRLRHRCCATLPLERPRLFEAAVQVGKHIQP"
     gene            complement(27458..27949)
                     /locus_tag="VFA54_10190"
     CDS             complement(27458..27949)
                     /locus_tag="VFA54_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51221.1"
                     /translation="MTNCKTRLSALLLLAFLPAVLPAQQSHGYLFIAPGGASGGGNTQ
                     ATMHMGAGGEWVPGKGIGAGAEIGALGPTSGFAENVLGVVSLNGYYHFLHDKRRVDPF
                     VTGGYSLLFRSGHANTGNFGVGVNYWFVPKIGAKLEFRDHVWPVDGSHIHYWDVRFGL
                     DFR"
     gene            complement(27946..28740)
                     /locus_tag="VFA54_10195"
     CDS             complement(27946..28740)
                     /locus_tag="VFA54_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS51222.1"
                     /translation="MLCDLTGKTALVTGASKGIGRGIALALARAGCNVAVNYHSDETG
                     ARSTAAEIEKLGRQAIIAAGHVGRSSDVDAMLASALAAFGRLHIWVNNAGVQTWSSLL
                     ELAEADWDRVIATNLKGTFLGTQRAARHMKEFGGGRIINIGSGCNKIPFPGLVDYTAS
                     KGGIEMLTKASAVELGPYGITVNCLAPGAIEIERTQKEAPDYAGAWAPLTPMRRIGKP
                     DDVASAVCFLASDEAEFITGQTLYVDGGLFTRGPWPYEKEQPNQTK"
     gene            complement(28791..28866)
                     /locus_tag="VFA54_10200"
     tRNA            complement(28791..28866)
                     /locus_tag="VFA54_10200"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28831..28833),aa:Lys,seq:ttt)
     gene            complement(28973..>30481)
                     /locus_tag="VFA54_10205"
     CDS             complement(28973..>30481)
                     /locus_tag="VFA54_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51223.1"
                     /translation="PDATAVIAPAFTGGRLLESRMSHRELADEASRAPSVLAAGDVVL
                     STTPLYTHEGRVEHYRAHLQGAAVAYLDPGEMSPALLGKVRPTRVVANAEFFTRLYTR
                     LAKRLEAGSSVHRRTWQWALEVGYEAAFQEAASLPLSARVKLQIADQVLLAELRGALG
                     GQLRQLVSLDAPIPNEQSVFFAAVGAACHYDPALKPAGAGTAAAPVASRPETRTPVPH
                     PMRDSRASAGIPFPAEFSLPPLERPDPVHPAAAARSHTLAGEKAPAKSETPPPGSGSS
                     GAVPLRWYGDPQGRSIPQSQAPASSPRFKEDLEEGGWLASLKNSGQAAWAWFRNVLGG
                     AAAILHRAGGRLGGLRRARASMQRNWMPSGERGEMPGLSRPALGPDAGAGAAEPVPSP
                     LEGDLHALAQRWAKSLVEPMVFFRAEQIQRGKASRNIYGLLKGEIDQGYELYQARFGR
                     VSMLGTDYYHLELVRALADGDEELMGPAYPFRRKPVQSEAGPNSGAGQRTAS"
BASE COUNT         5838 a         9352 c         9426 g         5865 t
ORIGIN      
        1 accggcaata tcatcagaat gacatcacct tcagcctcct gtattccttc accgagaatt
       61 tccttctgcc gatttcacac gacgaagtga ctcacggcaa gcgctcgctg atttccaaaa
      121 tgcccggcga tcagtggcag caattcgcta acgtcagggc gttcctcgca tatatgtacg
      181 gccatcccgg caaaaagctg ctgtttatgg ggacggaaat cggccagtgg aacgagtgga
      241 atcacgacaa gccggtcgag tgggagcttt tgcagtttga cgcgcaccgc tccttgcagc
      301 gctatgtcgc cgactggaac cggctgtacc ggcgcgagag aagcctctgg gaagtggatt
      361 tcgactggcg cggtttcgaa tgggtcgatt ttcacgacgc ccagaacagc atcattgtct
      421 tcatccggcg gcccgcgcca gcgatttccg gccagccgtc gccggccgag cgatctgccc
      481 cggaacagat ggcggcggca ggcagcgcgg aggcggcact gagcggcgag gcgccagagt
      541 ttttgctctt cgcttgcaac ttcactccgg tcgtccggca gggatatcgg atcggcgtgc
      601 ccgagcccgg ctggtatcag gaaattctga acagcgactc aaaactctac ggcggcagcg
      661 atgcgggaaa cgccggcggc gtcagtacag aagaaattcc ctctcataat cggccatatt
      721 cgatcgccat cactcttccc ccgctggctg tagtggtctt caagaagaca tagcgccgcc
      781 cgcggaatca caagccgatc ttcttctgat cgaaaaccgc aatgctaatc aggtgctctt
      841 aaactccaac gcccagatct tgccgcgtcc actccacagt caagcgcaga ataccggctc
      901 ctagggccca agtccgcgaa agcctcgctg gccggaattt tctcgagggc gacctggatg
      961 tccactccgg tcaacgccgg tagtcttcag aacgtgacct tgacgccgcg gcgcgcggat
     1021 tcgtaaatgc ccaaaataat ctccagagcg gcatagccct cttccccggc taccaggggc
     1081 ttgcggcggt tccggatcgc atcgccgaaa tccaaaaact gggccttgat cggctcaatc
     1141 gagattgcca tcgggtcagc cgctccggaa gccagcttcg tcgccagagg cacatcttcc
     1201 attttgtcgc cggctacgtc ccaggcagtg agccggtctc ccgtcaaaat ggccgaccct
     1261 tttgtgccgt gcaattcgat ccgctccgga tagccgggga aaaacgccgt cgagccctgg
     1321 atcacgcccg tcgctcccga agcgaaacgg atcacggcgt tcgcgatgtc ttccgactcc
     1381 attttatgcg ccgccccaag ctgccagtga ccgtagattt ccgcaaccgg gcccccaaac
     1441 caccggataa gatcaacgca atggatcgcc tgattgatca gagcgccgcc accctccacc
     1501 tgccagcttc cctttcccgg ccggtcgtaa tacgcctgcg gccggtgcca cttcacgtag
     1561 cagtcggcct cgagcagccg gccgagccgc cctgccgcaa gagctttatg aacaaaaatt
     1621 gcgccgggat cgaagcgatg ctggctgatt acgcccaggc ggatgtcggc cgcgcgcgcg
     1681 gtctcaatca tttgccgggc ggttgcgagg gatgtggcga tcggcttttg caccagcacg
     1741 tgttttcccg tccgggcgca ctcctcgact ggctggaggc ggaaatcggg aaaggtgcaa
     1801 acatcgacgt agtctatatc tctgcgccgg cagagatcac gatagtcggc gacaaattcg
     1861 gctccccact tttccgcgaa ctgcctgcct ttttcaacgg agcgattgtt gacagcaaca
     1921 agccggtacc ccgcggcgcg atacgcttca gcgtgcttgt gggcgatcgc cccggttccg
     1981 attaatccga cattcataaa gctggaagag ctcgccggtt tccgggccgg taaacccgca
     2041 gacaaccgcc ggcgcctgcg ttttagcgaa tctggcccga gcctaaggaa tcaaagcggt
     2101 gggcgggaca agccaaccaa ccgacctgga tcggctagac ctggaattcc cgcttcacct
     2161 tttcgctttt ttccgctaca ccccgcgcga gatcttcctt gaacttcgcc aggacgcctt
     2221 ctacgtgagg gtcggaggtg ccgatgattt gcgccgcgta gatagcggcg tttaccgcgc
     2281 cggccttgcc cacggccatc gtggccacgg gcactccgcc gggcatctgc accgtcgaga
     2341 ggagcgaatc catgcccgaa agcggcgagt tggcgatcgg aacgccgata accggcaggg
     2401 tcgattctgc ggcaatcacg cccgccaggt gggatgcgcc gcccgcgccc acgacaatcg
     2461 ccttcaaccc gcgtttgcgc gccgtgcggg catattcggc cgtgcgggcc ggcgacctgt
     2521 gggcgctggt tatctcaatc tcaaagggaa tagaaaattg cttcaatgtt ttcgcggtct
     2581 ccgccatgag gggcagatct gaatcgctgc ccatcacgat cccgaccagc ggcttgtgat
     2641 tttttgaatc gctcattgta atcgtgccag tccttttacg ccgatgtccc ggcggtagtg
     2701 cattccttca aatcgaattt tggcgacagc ggcgtaggcg ttgtcgatgg ccgcgggcag
     2761 atcttccccg cccgcagtga ccccgagcac ccggcctccg gcggtcacca gccgtccttc
     2821 cacaaatttc gttccggcat gaaacacttt ggcgccgagt tcctccgctt cgtcaattcc
     2881 aaaaatttcg tagccctgct cgtaatgtcc cggataccca gccgacgcca gcaccacaca
     2941 gaccgccggc cctgaagacc attttacagc tacgtcggtt atgcccgctc cctgggctgc
     3001 gcactgcaag agcgggacca gatccgactc gaggcggaac agcagggcct gcgcttccgg
     3061 atcgcccagg cgcacgttga attcgagcac ctgcggtccc tcggcggtca tcatcaggcc
     3121 gaaatacaag aagccccgat aggggcagcc tgccttatac agccggtcga gggtcggctc
     3181 cacgatgcgc tgcaagatcc cctgcttctc cgcctcgctc agtatcgccc catccgagta
     3241 cgctcccatg ccgccggtat taggcccccg atcgccgtca tagacggctt tgtggtcccg
     3301 agctgacggc agggcgtagt aaccggcgcc gtcgcttagc acgatgaagc tgacctcttc
     3361 gccgggcaga aaccgctcga gcactgcccg ccggccagcc ggtcctgcga tttttcccgc
     3421 cagcatcagt tccgccgcgg ctctggcctc gctttctttg tccagaatga gcacgccttt
     3481 ccccgcggcg aggccatccg ctttcaaaac cacgggaaac ccgaacgcgc cgatgttgcg
     3541 ggccacatct tctggcgact cgagcaccag gaaatcggcg gtcgggacgc cgctatcgcg
     3601 gagaaactgc ttggcgaaaa ccttgctgcc ctcgagccgc gccgcttggg cgtctggccc
     3661 cacaatccgc aggttgcgcg accggaattc atcggcaatg ccggcaacca gcggcgcctc
     3721 cggacccacg accgtaagat cggcgccgat ctctgccgcc agatcggcca tgagccgggg
     3781 ggaaacgata tcgccccccc ggcaaatggc ctcgctcgaa atccccgcat tgccaggcac
     3841 gcaatacact tcgtcggcca gagggctctg gcgcaatttc catacgatgg cgtgctcacg
     3901 gccgccggaa cctattacca gtatcttcat tgcttgccgg gaaccttgcc tccttgccgg
     3961 ctaattgcat agaataactg gaaacgaccc tttccgggat aactggatgg cgctttcctt
     4021 ccacgaaaaa tatgacgtag tcgtggtcgg agccggtcac gccggctgcg aagcctccgt
     4081 ggccacggcc cgaatggggc tgcggacggc gctggtgacc ctcaacctcg acctgatcgc
     4141 ccagatgtcg tgcaaccccg ctgtcggcgg catcgccaag ggccaccttg tccgcgaggt
     4201 ggatgctctg ggcggaatca tgggccaagt gaccgacgcc gtgggcatcc agttccggct
     4261 gctgaacacc agccgggggc cggctgtgtg gtcgccgcgc gcgcagtgcg ataagaaaca
     4321 gtatcgcatt gagatgaagc gccgcctcga ggccgagcgc aatctggtga tcaagcaggc
     4381 cgaggtcgta gatctggttc tcgaggagga ggccggtcca acgtttgacg gtcttcccgc
     4441 ccggcgcgtg cgcggggtag ctctccgcga tgggcggttg atcagcgccg gggccgtcgt
     4501 catcaccacc ggcacgttcc tcaatggcct catccattgc ggggagcaga cctatcccgc
     4561 aggccgcagc ggtgaaccgc cttcggtcat gctcggggaa cgattgcgga atcttgggct
     4621 gcgctgcggg cgtttgaaaa cgggcacgcc gccccggctg gacgcgcgat ccatcgattg
     4681 gagccgcttc gagatccagc cgggagatcc ggagccgacg ccgttttcgt tccgcacgga
     4741 caaaatcgag cgcgaacagg tggtctgtca tattgccttc accaccgagg aaacgcaccg
     4801 cgtcttacgg gataatctgc atcgctcccc gctctacagc ggccagatca caggcatagg
     4861 gccgcgctat tgcccatcca ttgaagataa gatcgtcaag tttcccgaca agccccggca
     4921 ccagattttt ctcgagccgg aagggcttga tacgaacgag atttacgtga atggcatgtc
     4981 cacaagcatg cctatcgacg tgcagacggc gatggtccat tcggtgcctg gtctggagaa
     5041 cgccgagatg atccggcccg ggtacgccat cgagtacgac tcggtggacg ccaccgagct
     5101 ggcgcctacg ctcgaggtca aaaaaatccg cgggttgttt ctggccggac aaatcaacgg
     5161 cacgtccggt tatgaagaag ccgcttgcca gggaatcatg gcggggatca atgccggctg
     5221 cgcagtcaag gggctgccgc cggtaacgct cgaccgcaca cagggctaca ccgggatttt
     5281 gatcgacgac ctcatcagca aaggaaccaa cgagccttat cggatgttca cctcgcgggc
     5341 ggagtttcgg ctgcacctgc ggatcgacaa cgccgatcaa aggcttaccc ctatcggaag
     5401 gcaagtcgga ctgatcgatg acgaggcctg gcaaaaattc tgcgccaagc aggaacgcat
     5461 ggcgcgcttc cggcagttcc tgaacgttta tcgccccgat ccccgcgacc acgccgccga
     5521 tcggttgctg aggaaagtca atcaaagcgc cggggattgc cctgtcgcaa gccaactgct
     5581 caaacgtccg gagatcgtga tcgaggacct ggcgccggtc ttgcaggaga aactcggtct
     5641 cccgttgaag aggctcgaac tcaaagccat cgaaacggag atcaagtacg aaggctatct
     5701 cgcccagcag cagaagcata ttgagcagct caagaaggcg gaaagccggc gcatccccgc
     5761 ggatttcccc tttcgcggcc tgccgggctt gtcgcgcgag gtcgtggaaa agctggaacg
     5821 agtgcggcca gtgaccctcg gccaggcggg gcggattccc ggggtcacgc cggcggccgt
     5881 ttccattatc aatgtatatc tcgacctgcc ggccaggcag ggggctcgca actgacgtcc
     5941 gatggatgag agtctagcct gcgagatccg gcccggcggc ggatgggatc tctctccggt
     6001 tcccaacgtt ccaggagtat ttgccgtttg gccttcacaa ggcccgccct acattggcaa
     6061 aagcagcctg ctgcggcgaa gactcgctcg gttgctccgg cctgccgaaa cgcaatcgag
     6121 gctgctgaat ctcgcgcaga tcgcctgccg ggtggaatac cagccggccg gatcgccgtt
     6181 cgaatccatg gtcctgctat acaagctcgc gcgccgccac cgccccggcg attaccaaaa
     6241 gtatctgaag ctgcgcattc cgccgttttt gaagattaat ctgagtaatg cctacccgcg
     6301 ctgctacgtt acgcataagc tcgggcaaag gcgggctttc tacttcggcc cttttccttc
     6361 acgggccgcc gcagagcgct tccagaacgc ctttctggat ctttttcaga tgcgccggtg
     6421 cgtggaagat ctggagccta ctccaacgca ccccggctgc atttacggcg aaatgaagat
     6481 gtgtctgcgg ccctgccagg cggctgtcac agcggcagaa tatcgcagcg agacggaacg
     6541 tgttgtgcag ttcctcgcga ccaatggcga atcgcttctc aaacaattgc gagacgagcg
     6601 ggaccgcgcg agcgccgcgc tccagttcga gcaggcagcc cggatccaca agaaactgga
     6661 gagggtggcg gaagctcttc agctcaaaga cgacctggcc ggagatcttg accgcctgca
     6721 cgccatcata atccagcgat ccgtggatcc ttgtgccgtc gagctctggc tgctgtatca
     6781 aggattcctg caaccacagc ggcgcttgca tgttgcggcg gaagcaggac agcccctgtc
     6841 tctggacagc aagctgcgcg aaactttgag cgcggttagc tgggaaacgg gcgctgcccg
     6901 gcagcgcgct gagcacctgg ccctggtggc gcgatggtat gcgagttcct ggaggcaggg
     6961 agaaatattg cgtttcgagg acttcgacca tctgccttac cggaagctgg tgaaggcgat
     7021 ctcgcgagtg gcggcaggat ccacacaacc ggcgggggcg cgcatttccg ggtcttatcg
     7081 gcccggttaa cgatgccgcg ccggagtctt cttctgctgc tgcaagaaag cggtcaaggc
     7141 ctgaagctgg cgctggttca ggttcgtggc tggcggcatg atggaacccc gcacataggc
     7201 gctgggattc tggaagtggc caatcagcca ggcgtccgtg cggccgcgat ttccttcatc
     7261 tgacaggtcc gggcccacgc cgcccccgtc gccgtccagc cgatgacatc ctatacagtt
     7321 ctcctggcgg aagaggagcc cgccctcctg gacaagatct tgcccgccgc tggccgcctg
     7381 aagagcagtc tcgggctgag gtttggctct ttgcaattcc cgaatggcca gcacgatctc
     7441 ccaggtttga gtgtcgtcga atacatcctt ccacgccggc atgcccgtga accgcacgcc
     7501 gttttgaatg atccagaaga gctcggcatc atcccaccgc ttcacgtgcg gggaggtaag
     7561 atcaatcgcc ggcgggtaca gatcgcgtcc gagcgtggtg ttcgagcgtc catcgctgcc
     7621 gtggcaaagc gcacaggacc ggcggtaaag atccatgcct tttttcacgg ctgcgccgtt
     7681 cacaggcaag ggatttttgc gttccttggc ctcgagcggg atcaccacct ccttcgcaat
     7741 gtttgccagg ccggattcca ccttggatgg gcggccgtcg gccgagcatc cagaatgaag
     7801 caatagtatc ggaaccgcac tcagcgcggc cagcaaaacc cggttttggc tcactaactt
     7861 tattacgggc ggggctcgcc gcctgttacg gtagctcaaa tttcttaacg gttgttgcgg
     7921 ccagaagcct cattctcttt gcgcctgccc ggcgcttgcg ggttaagcgg gccggctcgc
     7981 tggaaactct ccattgcgga ttgtgcgcag cgctgtccgg agttctccct tggctccgga
     8041 tctttttcca aaaaacacgc agtgaacggc ttgttttggt aaaaaacaag catgcacgaa
     8101 gacgcggaag ccgaaaacca gacgtcgagc gacaggggcg cccgcagggc cgttgagtgg
     8161 acggccaatc tcctgctggg cgccggctct ggcgggctaa tgggattgct gatagccggc
     8221 gtgatcggca tacgccacgg cccggacggc gcgccgctcg ctgtcacgct gctgctgacg
     8281 gccggcctgg ctgcaatcat ggcgtttctg agcgtgcgcc aggagcctcc tgcgcggccg
     8341 gtcgccccgc gcgtggcgtg gggtgtgctc ctgaccgggg ccgccctggt gattgcgctt
     8401 gccgtgtatt tgtttcaggg cgcgtatatt cagagcgtac gcggcagcgg aggccgggcc
     8461 gagcctcctc ttcccatttg gccggtttcg cgcgcggccc tcggggcatc cctggtgtct
     8521 ttgctggcgg gattagcggc cgcccttttg ggctgggctg aactggccgc gaatcgggaa
     8581 cgctatgggg gcggcaagtg ggtggcgctg tcggttctcg cagccggcgc gtggttggcg
     8641 ctggcgctgg cctgttacgt catcggttac ggctttacgt tcatttgagc gaggaagaga
     8701 ccgttgaaaa agagcttgta agtggcgtag ctctggccgc ggtattgcgg ccggatgccg
     8761 aacaaaataa tgtgcccgcg gccagccggg gcgtccatca cggcgctgcg gttttcgacc
     8821 agttccggtc ccagcagcca gcctgaaagc agtggattgc gccgcggata ctccacgacg
     8881 gatttggccg gaggattgga actcgcagga gccggctcga acgcgggtcc tgattcgcac
     8941 cacaccgctg ttggattttc catcccgaag gccaaggggt gcctggtatc cacatcggcg
     9001 gcaagcaggg atccaggagc atagaattcc cggttgggaa cgccggccag cacgttgcgc
     9061 gctccagcgc caagccgctc gatcgcgtag agggatgagc gattcagcgc cagcagtact
     9121 ccaccgcccc taacgaattc ccccaatgct tcggcgcccg ggtcccccag gccgccagaa
     9181 aactcagggg gcatgacgct tgtggatccg cggtccggtg ccgggctgaa gccggactcg
     9241 atgctttgcg gcgtcgcatc aggaagaacg atcacgtcga atcttgccag caggcgcccg
     9301 gctcgaatgt ccctgttgaa gagggttgta tatggaaact caaattgctc cagcagccag
     9361 cgcgtccagc cttcatccgt attgggcacg tagctctgat agaggccgat gcggggcagg
     9421 cgcagcctgg cgctcccgct tcgggagcgc gcggcggtga acggcaaccc gagatcccgc
     9481 gccagccccg gcagcaggga tttggcaggg ccgttgttgt cgataaggaa cgctccggtc
     9541 ttcagatcgc gccagacggc cgcgcccgct ttcagcaaac ggttcacggc gatccaggcg
     9601 ttggttagcg ctggagaaag cgccgcaacg gaggaaccgt ccacccggcc cggctccggc
     9661 tcgatcttct ccacacgatc caggacgaca tgaaaagggc tttcgatcgt ctcggcccgc
     9721 acacccatca gcagcggcag ggtttgggcc gccacatcgt agggacgcct tggcggcccg
     9781 ccgggataaa tccgcaggtc ggggtatcgc tgccgttcga gcagagtctt ggcataagag
     9841 ccataaggct gcgccagccg gatcacataa tcgccggcat cgtattcgcc gccgcctgcc
     9901 cgaaaggcaa cccgggctcg ctcgatttcg acctgtccga actgcaaggt ttcgagcatc
     9961 cgggtcatgg cgttgggatc atgctgccgg cgcgggatga tgaacgcgta aggcggatct
    10021 ttgcgctcca acgcgcggag gccgacgccg taaaagttgc gaagcaggtc ctcccgtcgc
    10081 aaggcggcct gataaagaca cgattcaaag gcgatcaatt ggtaatcgac aatgtcgcgc
    10141 agacgccatt cgccgcccgg ccagggctgc aagtaattcc aactgcgctc gcgggcattg
    10201 tagccaagag cgcccgtgtc gagctgatcc ggcgcaacat gaatcggcgt agccaggcgc
    10261 gcgctggccg attcggtgag aatgcgcaag ccgccgtgat acgcctgata atgccggcta
    10321 ggggtccaga aatcgtacat ggcgttgatg gctactcccg tcttgcccgc ggcagtcagg
    10381 tcagccgcaa tgcccgtgcc gatcatgttc atttcctgag cgattacagc gtccacattc
    10441 ggatccacgg gatcgagcca gggcgggaca aacaggcgcg cggcgtaagg ccccatctgg
    10501 tgcacgtcgt aaacaatctg cggatgccag acgttatgga ttttttcgac cgcaagacgc
    10561 gtctcgactt gcgtgaacat gtaccagtcg cggttattat catggccgac gtatttttgg
    10621 tagaggccgg gcggcggcgt gccttcccat ggcgtaccca attggcggcg gtaccagcgc
    10681 gtcaccagat ccacgccatc cgggttcagg gatgggacca gcagcaagat cgtgttcgcg
    10741 agaatcgaac gatggcgcgg cgtctcctct gcgagcagct tgtaggcgaa ctcgacggcg
    10801 gtgtgggtgg aggcgatctc cgttgagtgt atactgcacg tgatcaaaac gaccgtcttt
    10861 cccgcagcga tcagttgttc tgcttccgcc agattggtga ggcgcgggtc cgccaacttg
    10921 gcctgaatct ggcggtagcg gtcgagactg cgcagggtct ccgggtccga aataatggcc
    10981 agaataaaag gccgtccttc ggttgttttc ccaagctctt gcacgcggat ccggtcactg
    11041 gatcgctcca gcagcccgaa gtactcgacc accttcgacc actcgagcac agtgcggtcg
    11101 gcgcccattc tgtgaccgaa ctgcgattcg ggagatggga cggctgctct ggcggcgggc
    11161 gtccagattt ggataaaaag cagccagaag acgagtcgca agccttgccg gaacatccca
    11221 ccaatataac caagcccgcg gcggcgagcg ggaagagaag gcgccgatca aagagctcgg
    11281 gagttgcact gcttctgatg agaatcaact tggtgagccg gaccccatgg ggaagtgaac
    11341 cggccgcggg cagcgcggag cgcgcttgcg ttaaagcctc gggctgggga gaccgatgtg
    11401 gatgtgagcg cccgttgcgc tgccgagaac ggcgtgacgg aaagcgatgt aaggcacgct
    11461 caaacggtcc aacatgcgca tgagccagcg cccctcgggc tgatccggat tcaacgccac
    11521 gtctacgcgg tcccggtgat cgaaacccag actgcgatgt acggccgttt cgccccaggc
    11581 gcttacaggg aggggatgag aaaacttttg ataaaacgcc gactccaggc gcgccaaatc
    11641 gccggtggtg accgtgcctt tgccctcgaa cttggccgct aaggccaggt ccgctttcgc
    11701 ctgctcggcc atttcggatt cgcgccgggc catttcggct aattcctggg ttagcttggc
    11761 cctcgattcg gccaactgcc attgcttttc ggcctgcgct tcctccttct gggcgcgctc
    11821 cagcgccttg ggcgccagca cgccttcttt cacgagcttt tgctgctcgg ccagggcatt
    11881 ctgtgcccgc cgcagaagag cagacgccgc ccccaacatc cgcgggactt cctgcggcgt
    11941 tacgtcaccc cggtagagag tctcctggag gatctgctca tgccacgcgt cttcgagctg
    12001 ctcctcggcg gctttgagcg ccgaccgggg cgccgctccg gcctccacca gcatctgcac
    12061 gcggcgcagg ttggcctcgg ctgtagctac atcggccgat cgccccacag cggtgaacac
    12121 gaagagtaaa acaactgtga gcagcttccg catgaacttc tcattttacc cgcttttctc
    12181 cgcaccttgg atgccaggga aagcccattt tgcgtctaca ataacaaatg gggagtattg
    12241 gatggacttt tggcaggccg cggtggaagc ggcccgttcg aagaaagcag aagacatctc
    12301 ggtgctagac cttcacgaag ttgccagctt cacagacact tttgtcattt gcagcgggat
    12361 gagcgcccgc cagaatcagg cgattagcga cgaagtggaa agaaagctga aagagcaagg
    12421 attgcgcccg gccagcatcg aaggttatca gcaggcggaa tggattctga tggattacgg
    12481 cgacttcatc atccatatct tctcgcctcg cgcgcggaac tattatgagc tcgagcgcct
    12541 ctggaaaaat gcgcggcgca tcgccatcga gagggcggtt tgatgatgcc cccggctccc
    12601 tctacagtgg gtttcgagac caggccggcg acagccgctg acgcccctgt tctctggcgg
    12661 attttcaatc aggccatcga ggatcgcctc gcaacctttg ccgcccgggc ctgcccgctc
    12721 gacgaagtcg agggctggtt tcgcggcaag cgatttcacg cgctgctggc cgagaacttt
    12781 cgccagccgg tgggatgggc ctctttgcgc ccttaccgcg agggccccgc cttctcgacc
    12841 atcgccgaaa tgtctgttta tgtggatcgc acctggcgaa ggcatgccgt gggacaattg
    12901 cttgccgcag cgctgattga ggaagctcgc tctctcggct tgcataagct gatcggatac
    12961 ttgcttgaga gcaacatcag cagccgcaag ctggtggaga aactagggtt ccgcatggta
    13021 ggcgtacacc ggcggcacgg cgcgccggcg gccggctgcc cggacgtgat cgtggtggaa
    13081 aagctcctct gagcgggcgt gcgcgttttt ccttccgcta tggctgaaac aaaaggtctg
    13141 ttgatggttt ataccggcaa cggaaagggc aagacctcgg ccgctatggg atccgggttc
    13201 cgggcaatcg gccaagggtt gaaggtgctg atggtgcaat tcatcaaggg ctcctggcat
    13261 tatggcgagc tcgatgccgc caagatgctt ccggaaggtc tgttcgaaat tcgcgtgatg
    13321 ggaagaggct ttgtgaagat cggcgccgaa aagcccgatc cggccgacgt ggaaatggtc
    13381 gaaaaagcgt ggggcatggt cgtcgaagaa atgcacgcag gccggttcga catgatcatc
    13441 tgcgatgaaa tcaactacgc cattcattac ggaatgattg acatcgatag agttgtttcc
    13501 gaccttcggt cccgcccgga aacggtgcac gtgatctgca cgggccgtaa tgcgcacgcg
    13561 aagttaatcg gcgccgccga tctggtcagc gaaatcaagc agatcaaaca cccctatgag
    13621 aaaggcatcc tcgcccagcg gggcatcgaa tactagccat gccctggttc aagaaagaca
    13681 aggcgccgga gccgtccgaa ggcaagaagg ttcgcaccga aggcctgtgg atcaaatgcg
    13741 acggctgccg gcagattatc tggaagcgcg atctggaaac caatctcaac gtttgcccga
    13801 aatgcggcca tcacttcaga gtggacgccc gcacgcggct ggcccagttg ctcgacgagg
    13861 cgaagtggac agagctggat gccgacctcg cgtcgagcga tccgctctcg tttgccgacc
    13921 agaaaccgta ccgggagcgg ctctcggcca tgaaggcggc caccgggttg aacgacgcac
    13981 tgattaccgc tgagggttcg ctggccggac gcccggtgat tgtttgcgct atggaatacc
    14041 gctttatcgg cggcagcatg ggatgcgtcg tcggcgagaa gattacgcgg gccatcgaac
    14101 gcgcgatcga aacccggcgg cctctgctga tcgtatcagc ttccgggggc gcccgcatga
    14161 tggagggcgc cgtcagcctt atgcagatgg caaagatttc ctgcgccctg atgaaactgg
    14221 aagaagcccg cgtcccctac atcagcatcc ttaccgatcc gacgacaggc ggcgtcacag
    14281 ccagtttcgc catgctgggc gatttcaata tcgctgaacc gaacgcgctg atcggttttg
    14341 ccggaccgcg cgtgatcgaa cagaccatgc ggcaaaagct cccggccggc ttccagcgca
    14401 gcgagtttct gctgcaacat ggaatgctcg acgccgtggt aaaacgcggc gagcttaagg
    14461 cctggatcgc gcgagcgttg gatttcctct gcgggccggt gtaaaaatcg cgttggtcag
    14521 ccttcgcctt tccggcgccc ccgccggcca gggccggagc cgctttcggg gcgctggaag
    14581 cggcgtgctg acggtttcgc catgacttac cccgaagcag tgcagcgtct tttgcgtctc
    14641 ggcaacgagg ttaagaccgc caagctcagc ctggatcgga tggacgctct gctcgaaaag
    14701 ctggagcggc cggaacgcgc gttccgcagc gtgcatatcg ccggcaccaa cggcaaaggc
    14761 tcgacggccg ccatgattga agccggattg cgcgctgccg gacgccgcac aggcctctat
    14821 acctcgcccc acttggtccg gatcaacgag cgcattcagg taaacggaga ggaaatatcc
    14881 gacgccgatt tttgttccgt cttcgaaacg gtggagcgag ccgtcgagca actgcttgcc
    14941 caaggctccc tggagctgca tcctacctac ttcgagtgcc tgacggccat ggctttctgt
    15001 cattttcgat cggccggagt ggagcttgcc gtagtcgagg tggggctggg aggcaaactg
    15061 gacgcgacca atgtgctgct tccctccgtt tcggtcatta ctccgatcga ttttgatcat
    15121 gaggcgttcc tgggaaaagg aacagcttcg atttcggcgg aaaaggccgg aattttaaag
    15181 cctggcgtgc ctgccgtatt cgctccgcag agcccggaag cggaagaggt cctcgaggcg
    15241 cgggcgcgcg agctgcaaat ttgcgtagta cgcgcgggcc gggactggaa agccggccgc
    15301 atttcccacc accacgggta ttacgaattt gaggccgtca gcgacacagg ccggctgatc
    15361 gacgcccggc tgagcctggc tggcgagcat cagatcacaa acgccctggc ggcgattgcc
    15421 gcgctcgacc tgcttggtct gtctccggga gcgattgaac ggggattgcg cgtggcccgc
    15481 tggccaggcc gcctggagca ggcagcggag aaacccacgg tgctgctcga cggcgcgcac
    15541 aacccggcgg gagcccgcgc tctggcccgg tttgtgcggc agcactgccc tgggcgccgc
    15601 gtatggttga tctacgctgc gatgcgcgat aaggctgtag atgaggtcgc cggcatcctg
    15661 ttccccctgg cccacaaagt atttgtgacg ggtctggccc agccgcgcgc ggtaagtccg
    15721 gaagcgctcg ccgcgctggt ggcgcaccat catgtctgca ttcagaccac ggccaacgtt
    15781 gcggaggcgc tagccgcggc ccgcgccctc gcttccgagg ccgatgtgat tctggcgact
    15841 ggctcgctgt ttttggtcgg cgaagcaaag gcgctgctag catccagggc ccgatcggcg
    15901 gcatgagcat gttgcgcgcg ctcctgtgga ccgatccggc catttgcatt ctgacagtct
    15961 tcatgggcag catttccctg cttgcttcta ttgtcgatgg gacagggcac atgcaacacc
    16021 ggatcgcgcg ccgctgggcc cgcatggtga tggccgtgag cggcgctacg gtcagagtga
    16081 cggggctgga tcagctcacc ccgggcgcgg cttacgtttt ctgctcgaac catctgagct
    16141 tgattgacac gccgctcgta ttcggatacc tgccctggga gttccgcgtt ctggcaaaga
    16201 agatctactt tggcgttcca tttcttggct ggcatttacg ccgcgcgggc cacttgcccg
    16261 tcgagccgga caatgtgcgc gcgtcggtgc gcaatgtggc cgaggccgcc cggcaggccg
    16321 ctcttgggct gtcgattgtg atctttcctg agggcggccg cagccgcacg ggagccctgg
    16381 gcgagtttca cgccggggca gcctacattg ccatcaaggc gggggcgccg gttgttccca
    16441 tgggcattgt ggggacacga gaggtgcttc ccccgggatc aattatcgta cggcctcgcc
    16501 cggtcgagct gcgcatcggc gcacccattc ccaccgcagg cctgaccacc cgccacgcta
    16561 aagacttgct gctcgagctg cgccgcagga tcgtcgagct gagcggcgag ccggaacctt
    16621 ccgctcgaaa agaaatgccc gcgacgaagc actcatcctg agcggcgccg ccggcctgcg
    16681 ggcggcgcat cgaactggac cggaaccggc ggcttctgcg ttgtggccaa caggcagcgt
    16741 gttaccatcc agaaagagag gggcatgtac actcccgaag cggcttcttt ccatccgcaa
    16801 tccggcttga ccgcgcccag cgctcgagag cgggccgcgc gggctcttca tatgcgccat
    16861 ctggtcgagc agatacggca gatcgagagc cggttgcggg ccggaggcgg tccggcccgg
    16921 gtggaaaggc agcaccgggc cggcaagctt acggcccgcg agaggctgga aaagctgctg
    16981 gatccgggca gtcttttcct cgaggtgggc ttgctggtcg ccagcgacaa atacgacggc
    17041 caggcccctg ccgccggagt cgtgaccggc gtcggcttga tcgcggaacg gccggtcgtg
    17101 gtggttgcca acgacgcgac ggtaaaggcc ggcgcgtggt ggcctgagac catcacgaag
    17161 attctgcgct gccaggaaat tgccatgcgg cgccgcatcc ccattgtgta tctggtggat
    17221 tcggcaggag tgaatctccc ttaccaagac ggcatctttc cgggccaata tggagcaggc
    17281 cgcatttttt actacaactc gagaatgcgc cgggagctca aggtgccgca aatagcagcc
    17341 gtaatgggtc cttgcatcgc cggcggagcg tatctgccgg cgctcagcga cgtgatcatc
    17401 atggtggaga agaccagttt tatgggtttg ggcgggccga acctggtgaa gggcgcgacc
    17461 ggacaaacgg tggacgccga gagtctcggc ggcgccgaga tgcacaatac gctaagcggg
    17521 gtggcgcatt accgcgcggc gaatgacgcc gagtgcctgg ctttgatccg gcagttgatc
    17581 gacgaactgc cgcgccggcc ggcctctccc gctcacgagc cctcgccgcc tctgctgaac
    17641 gaggacgaaa tttacgagac gctgcccgcc gaccaccggc tgccctacga catccaggaa
    17701 atcgtgttcc gcgtttttga ccagcagggt tatctggagt ttatggcgga tttcgccccg
    17761 gaagtgcttt gcgccaacgc ccgggtgatg ggtttctcgg tcggccttat tgccaaccgc
    17821 cgcggcttct ttccatccgg cggcaagcct cgcatcggcg ggatcattta taccgaatcg
    17881 gcccgcaagg tatcgtattt cgtcgaaaat tgcagccgcc tcggctttcc gctcgtgtat
    17941 ttccaggacg tctccggatt catggtgggg ccggaagctg aacagagcgg gatcattcga
    18001 gcgggcgcag agatggtcga gactatgtcg tgcgccggcg tcccgaagat tattgtgacg
    18061 ttgaatcatg ccagcggcgc gggctactac gccatggcgg gccaaggctt tgatccggat
    18121 ttcactttta gctggcccac ggcccgcgtg ggagtaatgg aaggcgagtc ggcggttcag
    18181 gcgcttttcg cagcggagat cgaaaaagtg caagcttcgg gccaggccat gccggaagac
    18241 ttgcgggaag ccatcgagag aacgcgcgcc gattatgagc gttggctcga tgccaaattt
    18301 gcggcggcgc ggggccacgt ggatgcgatc atcgatcccc gagagacccg tcgcgttctc
    18361 gagctggcgc tccgcgtaac ctccggatga ggaggctcga gcgccgggtc gagtttcgct
    18421 tcacaaaccc ggaaaagcgt cttgatacgc ctgggcctgc tcacggacgg cctggatctg
    18481 caccagcggg ccgacgccga tgccgggaca gtaggttcgc agcttcttcc acgcgctgcg
    18541 gctggcgacg ttttccggcc agtaaggaaa ggtccggcat tggatgggct tgacatcatg
    18601 gatgctgcat cctccttcaa ccagaaaatg gcaagatcgg gagcgaggca gcctcagacg
    18661 cgggccgtat ttgccgcggc agacgtagcg cttttcaaac gccttcggat cgagttccaa
    18721 atacgcagca atgcggctga tatcttcttc cgttaggaaa atgtggccct tctgcgtgca
    18781 gcagttggtg cagcccttct ggcactcaaa gcggaaattg aacctgccga cctcgacgcg
    18841 accgctcatt gcgtcctcac ttccaaaacg gcatcttgcc ggatcagttt gcgcccgcga
    18901 agccattttt caaagtgttc ctgaatctgt tcggccgtca cggcgaagcc ggcctccagc
    18961 tccccatcgc ccgtcaccag agtttcgtcg aattcggaga ggccaatgaa attgcgcata
    19021 aaaaattgat ccatccactg cttgggaaca ggacggctct gctccccctc agccaggctc
    19081 agacggaact tacgcgcaaa caagctgcct ctcctctggc ggctctttcg cgggcttggc
    19141 cgccagcgtc ttctccatcg cccaggcatt ttcgcctgtg gcgtagtaac ggcgcaaaca
    19201 gcggattcgg gtgtagccga gcgaacgata cagggcttga gcgctgcggt tgcccacagc
    19261 cgtctctaag tggaccttcc tgactccggc cgcccgcagc cgctcctcgc agcagagcat
    19321 cagccgccgg cccaggccgc gcccccgcca agccggcagc acatcgagcg tgatgatatg
    19381 ggcggtgcgg ttgggttgct tgtaagcggc gacaaaaccg gccagacggc ccggggcagc
    19441 ctcggccacg atcgctaccg ctgcccggcg cccgagcagg gcagccattt cttttcgcgt
    19501 gtaagcgacg gctggatcga agcaagcgcg gtcgattgca cacagggcct cgaggtcgtc
    19561 cgggcaatag tcgcgaaggg tcatgggcaa aatttcttga cttcggataa tatttatcgt
    19621 ctaataaatc ttctcgtcaa gcaatgtccc ccggcgtaat cgagaagcgc atgaggctgt
    19681 tcggcgaccg ctgcgccgaa ctggggctgg cggccactca ccagcgccgc gccatttacc
    19741 gcgcgctcgc tgaaagcgag cagcatcccc agccggaggc gatttaccgc cgggtgagac
    19801 ggctcattcc gacgatctcc ctggccacag tctacaagaa catcaaaacg tttgtggaaa
    19861 tggggttgct gcgggaggtc ggctgcgaag ccggctcgta ccgcctggac gccaatctcg
    19921 accgccatca tcacctggtc tgcgtcaatt gcaagtcggt ggtggatttt tacgaccgaa
    19981 agctggacca ggtgcgggcc gggcaaacca gtcccggcaa ttttcgtatc ttccggtacc
    20041 aggtggaggc gctgggcctc tgcccggcct gccggtccaa acaaggtttg aggaaaaata
    20101 aaaagagagg acaatatggg caaagtcgct agacaagtcg ttgaacaagc tggtgtgaac
    20161 gtggacgtcc tgctggataa gctggtgcgc gcggcgggcg ccgagttcac cactttttac
    20221 tactacacca ttcttcgcgt caactccatc gggttcgacg gcgaagggct gaaggagatc
    20281 atcgaggatg cgcgcatcga agaccgcaac cacttcgaag cgctcacgcc ccgcatttac
    20341 gaattgggcg gcgccctgcc cagggacatt cggggctttg ccgatgtggc cggctgtccc
    20401 gacgcttatt tgcccgccga tccgcgcgat atgaaagcca tgctcaatgt gctggtcgag
    20461 gcggaacgct gcgccatccg cacctacacg gagatttgca acatgacttt cggcaaagac
    20521 caccggacct acgatcttgc tctggccatt ctgcacgaag aggtggagca cgaagcatgg
    20581 ttctcggaat acctcaacga agggccgtca gggcacttcc gccgcggcgc tccgggggct
    20641 tccccttacg tgcgccggtt catgacagcg gatttctcgt agagaagccg gtcatcgccg
    20701 ggcatccggt ttcagcgggg accctgcggc cacgatcggg cgcgcgctgc cgctggcccc
    20761 ggccgggtga aaacttccgg gttatgcctt ttcaaaaact ggcccgggcg gaggagattc
    20821 cgcccgggcg aaccaaattc ttgtgggcgg gcgagttccc tgtgatcctg gcgaatcttg
    20881 cgggccggat ctatgcgctt tacgggttgt gccctcatcg cgaaaatcct ctggaagggg
    20941 ctacgctctg ggatcacctg ctcgattgcc cctggcatca tttccaatac gacgtgagaa
    21001 cgggcgagaa ttatttcccc aaaaacgtgt atcccgcgga ttatccgcgc ctgcaagagc
    21061 aattgcgtcc gctcagaacc tttcctgtcg agatcaagga tggtgacgta tgggtgaatc
    21121 tggagtgagc agcctggctg cgaaacactg cgtgccctgc cgcggcggcg tgccgccgtt
    21181 gaaaggcgaa gagctgcgta aactcgccgc gcaggtcgcc gactggaagg tcgttgacga
    21241 gcatcatatt acgaagacct ttctgtttcc cgatttcaag accgcgctcg atttcgtgaa
    21301 ccgcgtcgga gcggtcgcgg agcaggaagg gcaccatccc gacctgactc tgacctgggg
    21361 tcgcgtcgat gtgaagactt acacccataa aatcgacggc ctcaccgaaa gtgatttcat
    21421 cctcgcggcg aaaattgacc agttgttttt caacaaatga gcctccgggc gccggccgcg
    21481 gcaacagagc gcggcgccgg aagcacccat agaaaaaaac tttcggccgc atagaatgtt
    21541 tcaatttgac tctgcctccg cgctctctta atctggtggg gccaggacac aaggccgtgt
    21601 cctgctaagc agcatctctc aggacctcga ctgtccttcc ttcactttgc tgtgattctg
    21661 aactgaagcg gaatctctgt ctccggcagc gtcgccgaga acgcgcgaaa aatggttcca
    21721 caccgagccc agctctcgtg aggccgcacg cctccaagcc ggcaggagca ctatgactat
    21781 gcagccaggg acaaatacga tcgagtcttt caaggcgctg aaaggcggcg atggcttgcg
    21841 gatccgcgaa gacctgccgc ggttgatccg cgacggctgg aaatccatga gccgggcgga
    21901 taaggacttg ttgaagtggg tgggcgtctt tttccggaat ccgacgccgg gccgctttat
    21961 gatgcgcatc cggatgccca acggcttcgc cgcgagcgag cagctccgcg ccatcgccga
    22021 gctctcccgg aggctgggca acaacatttt ggacataacg acgaggcagc aagtggagct
    22081 gcggggcttc acgattgaga gcgttccgga aatctgggaa aagctgcgcg gcgtggatct
    22141 gcacagcttg cagacgggaa tggacaacgt ccggaacatc aacggctgcc cgctcgccgg
    22201 attgaccccg ggcgagctct tcgacgcatc acctgtcctg ttggagctcg atcgcattct
    22261 ggtggggaaa gacggcaacc cggaattcac gaatctgccg cggaaattca acgtcaccat
    22321 caccggctgc ctggaaaatt gcacgcacag cgaatcgcag gatatcgctc tgattccggc
    22381 cagaaaaggc ggccgcgccg gcttcaacgt gctggccggc ggcaaaatgg ggtccggggg
    22441 gtttaccatc gcgacgccgc tcggcgtttt tgtcgagccg ggggaagcgg cacaggtctg
    22501 cgccgaattg atccgcattt accgcgacca tggccctcga gacgcgcgtt ccaagtgccg
    22561 gctggctttt ttgctcgagc agtggggggc gacacggctg cgcgccgagc tcgaataccg
    22621 cctcaaacgc ggcttggaga gcgctggtga ggatgcccgg cgggcgggtc ataccgatca
    22681 tctgggagtt acccgacaaa agcaggctgg gctggtcgcg gtgggtctat gcgtgcccac
    22741 aggccggctg gatgcggatc aaatgtcgga attggcccgc ctcgccgaag cttacggaaa
    22801 cgggcagatt cgttttacga cgggacagaa tgcaatcatt ccgaatgttc ctgcgcacag
    22861 gaccgcggcc ttgttccgcg aaccgctgct tgagcagttc tcgccggagc cgtccccttt
    22921 ctttcgcgga atggtggctt gtacgggaac cgatttctgc aatttggccc agatcgagac
    22981 caaacaaaag gctgtggaat tgtcgcgagc cctggccgac cggctcgggg atgacattgc
    23041 tcccttgaca attcactggt ccggttgccc agcggcctgc ggcaaccacc aggctgccga
    23101 catcgggttg cgcggcatga agatcaacgc ggggggaaag accatcgatg cggttgcgat
    23161 ttacaccggc ggccgcacgg gacccgaagc ggccgccggc gagcaaattc tggacgcggc
    23221 gcccctcgac gcgaacctgg cagatacaat cgccgccatt atccggcagg ccggcctggc
    23281 gcgccctctg aaacacaagg cggcgtcgcg aaacgcggcg cttgcgccag cggcgccgca
    23341 atttgttcct ttacagagta tcggcaacca gcagggagcg gaagctcgcg ccgtttcatc
    23401 cggcgtgtcc cggcaggtat gacagcagcc gcggcccgct gcgccggatc gtattctcgc
    23461 caggcggcga aggcgatttc tttcaaggtg ggccgaaagg cctgatcccg ggtgttgcct
    23521 gaacggaggt aatgaaatgg actacgtgaa tcccaaagag ctgatgacgg aagcattgga
    23581 tgtcgcggcg aagaaagcgg ccttacccgc cggcgacttg ctgttgcgtg gagcgctggc
    23641 cggaggtttg ctcggatatg ccacttcgct ggtggttgtg atccaaacgc aggggttgcc
    23701 cggaattgtc ggggctgcgc tgttccccgt ggggtttgcc atccttgtcc ttctcgggct
    23761 ggaactggct acaggcaatt ttgctctgct cccggcaggg ctgatggccg gcaggctgcc
    23821 gctgcgggcg ctcctgcgca actggctctg ggtctatgcc ggcaatctcg cggggagcat
    23881 cttgtacgcg ctccttttct atctggcggt caccaattgc ggcgcttcga gcgcggggcc
    23941 gctcggcgac caactgcgcc agatggccca gaaaaagacg ctcggttata tggcgctcgg
    24001 agccagcggg tggagtacgg ctttcatcaa aggcgttctg tgcaattgga tggtcaccct
    24061 gggagcggtc ctggcgctgg cctcgcgttc gacgattggc aagatcgtcg ccatgtggct
    24121 gccgattatg acgttcttcg cgcacggata cgaacattcg attgtgaaca tgttcgttat
    24181 gcctgcgggc atgttgtttg gggcgccggt ctccgtctca agctggtggc tgtggaatca
    24241 gatccctgtc accatgggca acatttttgc gggggcgctg tttacgggcc tcgctctcta
    24301 tgcgacctat gcggcgaagc tgccgtccgc agaagcccct ctcaatctgc cggagcggga
    24361 agcggccttc gcatcgacat tgagaggctg acaacgatga gagcacaatg cttccccctg
    24421 gccctggcgg cggctgtgtt cctggcgcgg gggctgtccg cccagcagac cgccgcacca
    24481 tctcccgccg gatcgagtcc cgtcagtacg agcctggtcc cgtccgacga gctgaataaa
    24541 gagctgccga agtggctgcg cttcagcgga gaatatcgag gccgcgcgga aggcttcacc
    24601 ggagccggat ttaagcccga cgtcgatgac gattactggc ttagccgcct gcggctgaat
    24661 atgaaaatcc agcccacgga ctggctgaag ttcgggtttc aggcccagga cgcgcgggtt
    24721 tttggcaagg atcagagccc ggcggcccct ccctaccaga acacgatgga cctccgcctg
    24781 gcctatatcg aaatcgggga tgccgaaaag aaaagcttcg gcctgagagt ggggcggcaa
    24841 gaactggctt tcggcgagga gcgtctgatt gggaatgtga actgggtcaa cacggcgcgc
    24901 agcttcgacg ctgtgcgggc cacgctgcgc cgcggcaagt accggctgga tgcgtttgcg
    24961 tcctcagtcg tcgtggcgaa agacggctcc ttcgatcaca gccaggcggg cagcgatctc
    25021 cacggcctct atggaggaat ggaaaatgtc gtccccggct caaccattga gccttacgtg
    25081 ttctggcggc tggcgcgcgc agtggtcagt gaagaaggaa cgccaagcca tctgaatttt
    25141 aagaccgtag gttttcggtg ggtcggcaag cttccgctcc actttgatta tggcgccgag
    25201 gtcgccggcc agattggatc gctcggagcc gacaacgtgg gcgcctgggc cgggcactgg
    25261 cttgccggct acacgctcgc gaatctgcgc tataagccgc gtctggtggc ggagtataac
    25321 ttcgcctccg gcgacaagaa tccgcgtgac ggcgcccgcg gcacattcga ccagctctat
    25381 cccaccggcc atgacaagct gggcctggcg gatcaggtgg gctggcggaa tatccatcac
    25441 gtgcgcgcgg gagtcgagtt ccggccccag gcaaagttac tggtaatcgg taaatataat
    25501 gcttattggc tagacagtgc gcgcgacgcc ctgtacaacg cggcaggcgc ggccgtcgct
    25561 cgtgtcgccg acggctcggc cggaaagtat gtggggcagg aagccgattt ccaatcggtc
    25621 tacacgttct ctcgccagct ccaattcgga ggcggttatg cccgcatggc gcccggaacg
    25681 tttctgaaaa aagccactcc gggcgcgtcg tacaactacc cctacatgct gtttacgtac
    25741 ttgttctgaa gtttccgcga gtttctccaa ggctttgccg cccacgcaaa acaatgcctg
    25801 tgcacattta caaaacgtgt gaagcgcctg cctgggagcc gctgttccgg ccgttttcag
    25861 gcacgcagcg cttgagagtc tttggcagcg tcgcgatcgc cctctggcgg catccccacg
    25921 cagggccggc agggcgatct gttcaggcca ggatggccgc cgagcccgct cgccgcgccc
    25981 agaagccgcg ctgattcccc gccgaatcct ctttccgtct ccataatgaa gccgcgaatt
    26041 tttgcggtat atttcgccgg ttttcaccgc ggtgtaacta tgccgcggat acgctgaaag
    26101 gtgaatatga gacaaggtta tttgatcgac atggatggcg tgatttatcg cgggtccgag
    26161 ccgattcccg gggccgccga tttcattctt tacttgcagg agcaagggat tccttatctc
    26221 ttcctgacca acaactcggc ttatacgccc gtcgacgtcg ccgtgaaact ccggaaattc
    26281 ggcatcgaaa cctccccgga gcacgtctat acgtcagctt tggccactgc cgagttcgtc
    26341 catcggcaaa agccgaatgg cactgctttc gtaatcggcg agggcggctt gctgagcgcg
    26401 ctcaacgacg tgcgttacgc catcagcaga gaatcgccgg attacgtgat cgtgggagaa
    26461 gggcgcgtct tgaattttga gttggccgag caggcccatc gcctgatcgc ggcaggcgcc
    26521 cagttgatct ccaccaacgc cgacacctgg tgccctaccg attccggtcc ccggcccggc
    26581 tgcggcgcta ttgtggcttt gctcgaggcg gccaccggac gcaaggctta tcacgtcggc
    26641 aaaccgaatc cgttcatgat gcgcacagcc cgcaagctga tcggcctgcg gaccgacgag
    26701 gtcgttatga tcggcgacac gatggaaacc gatatcaaag gcgccgccga gctcgggttc
    26761 cgcagcattc tggtgctcac cggatcgagc acgcgggaaa gtctgaggga tcatcctttc
    26821 tcgccgacgc aggtggtcga gtccgtcgcg gcgcttgtcc cggaatacat tatgggcgaa
    26881 cgccgggtag gggaacaccg caatggctcg gccttccgct acggctgaat gtgcttcccg
    26941 acttggaccg cggcctcgaa caggcgcggg cgctccaacg gcagcgtggc gcaacatcga
    27001 tgccggaggc gaacagtttt ctcgaaccct tcatagaccg ccggcttggc cacaaacggc
    27061 acggagttac gtccttgttc gcggcgctgg ccgtggcggc gggaatcgcg atcggcaatt
    27121 gctgccggcg tcaccgccat caggagttcc tgcggttccc cgatgacatc gatgcgattc
    27181 cgcccggcgg cttcgatgtg catctggcaa tggacaatcg gggttctggg gcgtataggt
    27241 tcatgcgcca agcaagcgta aacgaagcgg ctgggccctg gtcttggacc gccatgttgt
    27301 caaagaccca ggtgaatgac caatgcacgc ggacaagcgc cactgatcgc cggttcgacg
    27361 gtaacctggg ttgagttcca gcggaaggac gggaatttgc ttatatccgt cgggcgcctt
    27421 gcgcaggctt ggagccatcc tcccgcacgg cgagatgtca gcgaaaatcc agcccgaaac
    27481 gaacatccca gtaatgaata tgagagccgt ccacaggcca gacgtggtcc cgaaattcca
    27541 acttcgcgcc gattttcggg acgaaccaat agttcacccc aaccccaaag tttccagtgt
    27601 ttgcgtggcc ggagcggaat agcagcgaat atcccccggt aacaaacgga tccacgcggc
    27661 gcttgtcatg aaggaaatgg taatagccgt tcaaagaaac aaccccaaga acgttttccg
    27721 caaagccgct ggtggggccg agcgcgccaa tttcggcgcc cgcaccgatc cctttgccgg
    27781 gaacccattc gccgccggcg cccatgtgca tcgtcgcctg ggtgttcccg ccgccgctgg
    27841 cgccgcccgg ggcgatgaag agatatccat gagactgctg ggcgggcaag acggcgggaa
    27901 ggaacgcaag caacagcagc gcggagagcc gcgttttgca gttggtcatt tggtctggtt
    27961 tggttgctcc ttttcatagg gccaggggcc gcgcgtgaat aagccgccgt ccacataaag
    28021 ggtctggccg gtgataaatt ccgcttcgtc cgacgccaga aagcaaacag cggacgcgac
    28081 atcatcgggc ttgccgatcc ggcgcatcgg agtcagcgga gcccacgcgc cggcgtaatc
    28141 gggagcttcc ttctgcgtgc gctcaatttc aatcgccccc ggcgcaaggc agttcaccgt
    28201 aatgccgtat ggcccgagtt cgaccgccga cgccttcgta agcatttcaa tgccgccctt
    28261 gctggccgta tagtccacga gtcccggaaa gggaatcttg ttgcagcccg agccgatgtt
    28321 gataatgcgc ccgccgccga attccttcat atggcgggcg gcgcgctggg ttccgaggaa
    28381 ggttcccttc aagtttgtcg caatcacgcg gtcccagtcg gcttctgcca gctccagcag
    28441 agagctccag gtttgcacgc cggcgttgtt cacccagata tgcaggcggc cgaacgcggc
    28501 aagagcgctt gccagcattg catcaacatc gctcgagcgg cccacatggc cggcggcgat
    28561 gatggcctgg cggcccagtt tttcgatttc cgcagcggta ctccgggccc cggtttcatc
    28621 cgaatggtaa ttcacggcaa cattacagcc cgctcgcgcc agcgccagcg caatgccgcg
    28681 cccgatgccc ttgctggcgc cggttaccag cgccgtcttg cccgtcagat cgcacaacat
    28741 ttcacgtcct taaaacttca tcctaacgta ggaaaaggcc gattgcaggt tggtgaggcg
    28801 ggcgggactc gaacccgcga cccacggctt aaaaggccgt tgctctacct actgagctac
    28861 cgccccctgt gcggagggaa gattcagcgt cctttcatta tacgggaacg gagcagcagt
    28921 taccggaaca gtcgggctag ccggacgcga agcgaatgcg agccttggga cgttacgaag
    28981 cggttctttg gcccgctccg gaattcgggc cggcttccga ttgaaccggt tttctgcgaa
    29041 atgggtaagc tggtcccatc agctcctcat cgccatccgc cagggcgcgc acgagttcca
    29101 gatgatagta atcggttccc agcatgctca ctcttccgaa gcgcgcctga taaagctcat
    29161 agccttggtc gatctctccc ttaaggagtc cgtagatatt tcgcgaggcc ttgccgcgtt
    29221 ggatctgctc ggctcgaaaa aaaaccatcg gctcgacaag actcttggcc cagcgctgcg
    29281 ccagtgcgtg cagatcgcct tccaatggtg aagggacagg ctcggctgcg cctgcacccg
    29341 catccggccc aagcgccggg cgggagagac ctggcatttc acctcgctct ccggatggca
    29401 tccagttccg ctgcatgctt gcccgcgccc tgcgcagccc tcctaagcgg ccgcccgcgc
    29461 gatgcagaat tgccgcggcg cccccaagca cgttccggaa ccacgcccag gctgcctggc
    29521 ccgagttctt cagagaagca agccagccgc cttcttccaa atcttccttg aagcgcgggg
    29581 aagaggccgg cgcctggctc tgtggaatac tccgcccctg cggatcgcca taccaccgga
    29641 gcgggaccgc ccctgaagag cccgatccgg gcggcggcgt ttcgctcttc gcgggcgcct
    29701 tttcaccggc aagtgtatgg gagcgagcag cggcagccgg gtgaaccgga tccggccgct
    29761 ccaagggcgg caagctgaac tctgccggaa acgggatccc ggcagaggcg cgactgtctc
    29821 gcatcggatg tggaactggc gtgcgcgtct cgggccggga cgcaaccggg gccgcagcgg
    29881 taccagcgcc ggccggtttt aaggcgggat cgtaatggca tgccgcgcca acggctgcaa
    29941 agaacacgct ctgctcgttc ggaatcggag catccaacga aaccaactgg cgcagttggc
    30001 ctcccagcgc gccgcgaagc tccgcaagga gcacttgatc cgcgatctga agtttcacgc
    30061 gcgcgctgag cggtagactc gcggcctcct gaaacgcggc ttcatagcca acctcgaggg
    30121 cccattgcca cgtccggcga tgcacggaag accccgcttc caggcgtttc gccagacgag
    30181 tgtagaggcg tgtgaagaac tcggcattcg ccacgacgcg cgtgggccga accttcccga
    30241 gaagcgccgg tgacatttct ccggggtcga gatacgcaac ggcggcccct tgcaggtggg
    30301 cgcggtaatg ttccacgcgg ccctcatgtg tgtagagcgg cgtcgtggaa agaacaacgt
    30361 ccccggccgc aagaacagac ggcgcccggg acgcctcgtc tgccagctcc ctgtgactca
    30421 ttcggctttc cagcagccgg ccgccggtaa aggcgggcgc aatgaccgcc gtggcatccg
    30481 g
//
LOCUS       DASREK010000196         5799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1084074, whole genome shotgun sequence.
ACCESSION   DASREK010000196 DASREK010000000
VERSION     DASREK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084074"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFA54_10210"
     CDS             <1..320
                     /locus_tag="VFA54_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZS51224.1"
                     /translation="VDELDLLHHEGDQHYYETRLKREHAHVVCLRCGRVEEFFGEALK
                     RLKQQIESHLGFHIVVARTEVGGYCANCQMMAARESEELRNGSVPLREQASGAGPKSR
                     ARK"
     gene            324..2018
                     /locus_tag="VFA54_10215"
     CDS             324..2018
                     /locus_tag="VFA54_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZS51225.1"
                     /translation="MGSLPSSVPTAELFSETSRARPFLAVVEPGGCRQLVKISKSPFQ
                     IGRLAECEVSLRDSRISRQHAQIVAEDDSYYLEDLNSRHGTFVNGTKITRHKLSANDR
                     IDFGVEDSYHLVFTYETGDTERLLAQLDAVPTTHATGNLAKLRAVLEVARALESSMSL
                     EDVLGAVVDAALVVTGAERGFLLLHNSQGTALEMRVARDKHGQPLDETDLRVPRGVIQ
                     RALHQRRDLLSMNFDPAQINTEKSVVDLELRSVVCVPLVRIRIGHQHETSQLSTKNDT
                     LGVLYMDSRVGSADLSAGNRELLQSLAIETSTVLENARLMSQEREKRRLEQELSIARE
                     IQRSLLPSRLPSDGWFRAAGSSQACFQVGGDYYDVMELSESLWGFVLADVSGKGVSAA
                     LLTSLLQGAFFATAGHESRLTEIMSRVNRYVCERSQTRNYATVFYCTVDRSGATRWIN
                     AGHCPPLLVRAAGKMEELAASACPIGLFPAAPFAQQETQLLPGDKLVVYSDGVTEAEN
                     AAGEQFGEEALMTLVRKRAGAPAAELHQAIIDEVSRFTAGAPQSDDVTLVVLEYRG"
     gene            complement(2173..3846)
                     /locus_tag="VFA54_10220"
     CDS             complement(2173..3846)
                     /locus_tag="VFA54_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZS51226.1"
                     /translation="MSGLLPRRTWLQLLAVLAAAPGPQQPQQPQPAPQGITLETLQHA
                     LKLIGIQMSEAQQAMMLPGINRALAQYVSLRQIPVPLDTEPAFHFSPLLPGKLIRTGP
                     ARFRPSRHAAPKRSSWKSVEDLAFLPVTELGPLIRERHVSSVELTKMYLNRLKTYGPK
                     LHCVITLTEDLALAQAEEADREIRAGRYRGPLHGIPWGAKDLFATKGIPTTWGAEPFR
                     GQLLDYDAAVVERLREAGAVLAAKLSMGALAQGGLWFGGMTRTPWDLEQSSSGSSAGS
                     ASATAAGLVGFALGTETLGSIISPSIRCGVVGLRPTYGRVSRYGAMALSWTMDKIGPI
                     CRSVEDCALVLHAIYGPDGRDTTVAPVPFDWEPRLPLKELRVGILQKDFDGLQGEEKA
                     VYDKALEDLEKAGVTPEPIELPPISGETLRFILSAEAAAAFDDLTRNGGVDQLRGQNP
                     GDWPNTFRTSRLIPAVEYIRAQRARTLLMRQMEDLMSQWDAFVCRPGPSLTVTNLTGH
                     PQVVVPCGFVKGLPQGLVFTGRLYEEGVPLRVALAFEQATEWHKMTPKL"
     gene            3970..4431
                     /locus_tag="VFA54_10225"
     CDS             3970..4431
                     /locus_tag="VFA54_10225"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZS51227.1"
                     /translation="MSTRDYHSMLEIAIEEARLGLAEGGIPIGAALFDRAGRLLGRGH
                     NRRVQANDPSIHGETDAFRKAGRQKTYRDKIMVTTLAPCWYCSGLVRQFHIGTVICGE
                     SVNFSGGVSWLRQNGVEVIDLHSQECIQMLADYIRAHPEIWNEDIGEEQPS"
     gene            4537..5208
                     /locus_tag="VFA54_10230"
     CDS             4537..5208
                     /locus_tag="VFA54_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS51228.1"
                     /translation="MAKKILLSWSGGKDSAWALEVLSHSEEIAVAGLLTTFNEAFDRV
                     AMHAVRRTLIEAQAERAGIPLWDVPLPWPCPNQEYERILKRTCERAVAAGIEAIAFGD
                     LFLADIRAYRETQLRDTGLEPLFPIWGRPTRELAREMIAAGLRARLTCVDPRVLPRSF
                     AGREFDAALLEDLPAEADPCGEHGEFHTFVYDGPMFRRPISVALGEIIERDGFVFADL
                     LPFRE"
     gene            5390..5671
                     /locus_tag="VFA54_10235"
     CDS             5390..5671
                     /locus_tag="VFA54_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51229.1"
                     /translation="MTGSQSYGAADARAVYRLAAAILVQAIEDIRSGSHNKRADAVRW
                     VTDPTDRQFSFTFCCRVLSRNPEQVRRFLLAQVPPARWSLRIDAPRFSV"
BASE COUNT         1084 a         1833 c         1780 g         1102 t
ORIGIN      
        1 ttgttgacga gctggacctg ctccaccacg agggcgacca gcactattac gagacccgcc
       61 tgaaacgcga gcatgcccac gtggtttgcc tccgttgcgg ccgcgtggag gaattttttg
      121 gtgaggcgct caaacgcctc aagcagcaga tcgagtctca tttggggttc catatcgtcg
      181 tcgcccgcac ggaagtcggc ggctactgcg ccaattgcca gatgatggcg gcgcgtgaat
      241 cggaggaatt gcgcaacggg agcgtcccgc tgcgggaaca ggcctcgggc gccggcccga
      301 aatcccgtgc caggaagtaa cagatggggt ccttaccctc ttcggtccct acggccgagt
      361 tgttttcgga aacttcgcgt gcgcgcccct ttctggcggt cgttgagccg ggcgggtgcc
      421 ggcagctcgt gaagatttcc aaaagcccct ttcagatcgg ccgtcttgcc gagtgcgagg
      481 tgtctctgcg cgatagccgc atctcgcgcc agcacgccca aatcgtcgcc gaagacgact
      541 cctattatct ggaagacctc aacagccggc atggcacatt tgtaaacgga acaaaaatca
      601 cccggcacaa gctttcggcc aacgatcgca tcgatttcgg cgtggaagat tcgtaccacc
      661 tggtcttcac gtacgaaacc ggggacaccg agcgcctcct cgcgcaactg gatgccgtac
      721 ccactaccca tgccacgggc aatctggcca aattgcgggc cgtgctggaa gtggcccggg
      781 cgctggagtc ttccatgtct ctggaagacg tcctgggggc cgtggtggac gccgctctgg
      841 tcgttacggg cgccgagcgc ggctttctcc tgcttcacaa cagccagggt acggccctgg
      901 agatgcgagt ggcgcgggat aagcacgggc agcccctcga cgagacggac ctccgcgtgc
      961 cgcgcggcgt gatccagcgc gcgctgcacc agcgccgcga tctgctttcg atgaactttg
     1021 accctgccca gatcaatacc gaaaaaagcg tggtcgatct ggaactgcgg agcgtggtgt
     1081 gcgtccccct ggtccgcatc cggatcgggc accaacacga gaccagccag ctttccacca
     1141 agaacgacac gctgggcgtg ctttacatgg attcccgcgt gggctccgcc gatctctcgg
     1201 cgggcaatcg cgagctgctg caatcgttgg ccatcgagac atccaccgtg ctggaaaatg
     1261 cccgcctgat gtcccaggag cgggaaaagc ggcgtcttga gcaggaactt tccatcgccc
     1321 gggaaattca gcgcagcctt ctgccgtcgc ggctgcccag cgatggatgg ttccgcgcgg
     1381 cgggcagcag ccaggcctgc ttccaggtag gcggggatta ctacgacgtc atggagctct
     1441 ccgagtcgct gtggggcttt gtgctggccg atgtctctgg aaaaggcgtt tcagccgccc
     1501 tgctcacctc gctgctgcaa ggcgctttct tcgccacggc cggccacgaa tcgcggctca
     1561 ctgagatcat gtcccgcgta aaccggtatg tgtgcgagcg ctcccaaacc cggaactacg
     1621 cgaccgtgtt ttactgcacc gtcgatcgca gcggcgctac gcgctggatc aacgccggcc
     1681 actgccctcc actgctggtg cgcgccgcag ggaagatgga ggaactggcc gcttctgctt
     1741 gtcctatcgg cctgtttccg gcggcgccgt tcgcccagca ggagacccag ttgcttcccg
     1801 gcgacaagct cgtcgtttac agcgacggcg taaccgaagc cgaaaatgcc gcaggagaac
     1861 agttcgggga agaggcgctg atgacgctcg ttcgcaagcg cgccggcgcg ccagccgccg
     1921 agctgcacca ggctatcatc gacgaggtct cccgatttac tgcgggcgcg cctcagagcg
     1981 acgatgtgac tctggttgtc cttgagtatc gaggataggt ttcgggccgc caaagaatct
     2041 accacggctg aacccgagct tggcttcttc cgggaacgat cttcggcctt ttctctctcc
     2101 gccgaagaaa atgaatttac aaatgccgct ggctcccggt cggccgacag agccgccatg
     2161 cggtgcaacc catcacagct tcggcgtcat cttgtgccac tcagtggctt gctcgaacgc
     2221 cagcgcgacc cgcaacggca cgccttcctc atacaggcgt cccgtgaaaa ccagcccttg
     2281 cgggagaccc ttcacaaatc cgcatggcac gacgacctgg ggatggcccg tcaggttggt
     2341 caccgtcagg ctgggtcccg ggcggcagac aaacgcatcc cactgcgaca tcaggtcttc
     2401 catctgccgc attaacagag tgcgggcccg ttgcgcccgg atatactcta cagcgggaat
     2461 cagacgggat gtccggaacg tattaggcca atcgccggga ttttgccccc gcaattgatc
     2521 gaccccgccg tttcgcgtca gatcgtcaaa cgcagcggcc gcttccgcac tcaaaatgaa
     2581 tcgcagcgtt tcgcccgata tcggcggcag ctcgatcggc tcgggggtga ccccggcttt
     2641 ttccagatct tccagtgcct tgtcgtagac cgccttttcc tctccttgca gtccatcgaa
     2701 atccttctgc aaaatgccca cgcgcagctc tttgagcggc agccggggct cccagtcgaa
     2761 tggaaccgga gccactgtgg tatcccgccc atccgggccg taaatggcat gcaaaaccaa
     2821 ggcgcaatcc tccacggaac ggcagatagg accgatctta tccatggtcc agctcagcgc
     2881 catggcgccg tagcggctga ctcttccata cgtcggccgc aagcccacca cgccgcagcg
     2941 gatggagggg ctgatgatcg atcccagcgt ttcggtgccg agggcgaagc cgacaaggcc
     3001 ggcggcagta gcagaggccg atccggccga ggaaccgctc gaggactgct cgaggtccca
     3061 cggcgtccgc gtcatgccgc caaaccataa gccgccctga gccaaagcgc ccatcgagag
     3121 cttggctgcc agcaccgcac ccgcttcccg gagccgttcc actacggccg catcgtaatc
     3181 cagcagttgc cctcgaaaag gctccgcccc ccaggtcgta ggaataccct tcgtggcgaa
     3241 aaggtccttc gcgccccagg ggatgccgtg gagcgggcct cgataccggc cggcgcggat
     3301 ctcgcgatcg gcttcctcgg cttgagccaa cgcgaggtct tcggtcaggg tgatcacgca
     3361 gtggagctta ggcccgtagg ttttcagccg attcagatac atcttcgtca gttcaaccga
     3421 ggaaacgtgc cgctctcgaa tgaggggccc aagctcggtc accggcagaa aggcgagatc
     3481 ctcaaccgac ttccacgaag accgtttcgg agctgcgtga cggctaggcc ggaagcgggc
     3541 gggccctgtg cggatcagct tgccgggcag cagcgggctg aaatgaaaag cgggctcggt
     3601 atccagcggc acagggattt gccgcagcga gacgtactgc gccagcgcac ggtttatccc
     3661 cggcagcatc atggcttgct gggcttcact catctgtatt ccgatcagct tgagggcatg
     3721 ctggagagtt tctaacgtga tcccttgggg cgccggttgc ggctgctggg gctgctgcgg
     3781 cccgggcgct gcggccagaa cagcgagaag ctgaagccag gtcctacgcg ggagcagacc
     3841 gctcataaag tacagaacga ttatagcggc agccggccga gccgctctct gccaggtggt
     3901 ttgctacgtt gaaatccggc gcggcccttg ctggcccacg ggtcggcctc tttgttgtaa
     3961 cttctatacg tgtctactcg cgactaccat tccatgctgg aaatcgccat cgaagaagcc
     4021 cgcctgggat tggccgaggg cggcatcccc atcggcgcgg ccctgttcga tcgcgccggc
     4081 cggctcctcg gccgcggcca caaccggcgc gtgcaagcga atgatccttc catacacggc
     4141 gaaaccgacg cattccggaa agccggccgc cagaaaacgt accgggacaa aatcatggtg
     4201 accacgctgg cgccgtgctg gtattgcagc ggcttggtgc ggcagtttca cattgggacg
     4261 gtgatctgcg gggaatcggt gaatttttcg ggcggcgtct cctggctgcg gcagaacggc
     4321 gtggaagtga tcgacctcca ttcccaggaa tgcattcaga tgctggctga ctacatccgg
     4381 gcgcacccgg aaatctggaa cgaagatatc ggggaggagc agccgtcatg acccgccgat
     4441 cgccggcagg acccaggcaa cgcttgcatc cggttaaggg actggcaatt ccggcggcga
     4501 gtcccaggtt gatccagcgg ctggagcgct cctgacgtgg caaagaaaat tctgctctcc
     4561 tggagcggtg gaaaagacag cgcctgggct ctcgaagtcc tctcgcactc ggaagaaatt
     4621 gccgtggcgg gcctgctcac cacattcaat gaggcctttg accgcgttgc catgcatgcg
     4681 gtacggcgca cgttgatcga agcccaggcg gagagggctg gtattccttt gtgggatgtc
     4741 ccactgccat ggccgtgccc gaaccaagag tatgagcgga ttctgaaaag gacctgcgag
     4801 cgagccgtcg ccgcaggaat tgaggcgatt gcgttcggcg atttgtttct ggccgacatc
     4861 cgcgcttacc gcgaaacaca actccgcgat accggcctcg agccgctgtt tcccatctgg
     4921 gggcgcccca ctcgcgaatt agcgcgggaa atgatcgccg cgggcctccg ggcacggctc
     4981 acctgtgtgg acccgcgggt tcttccccgc agctttgccg gccgcgaatt tgatgcagcc
     5041 ctcctcgagg atctgccggc ggaggccgat ccctgcggcg agcacggcga gtttcatacg
     5101 ttcgtctacg atggcccgat gtttcgccgc ccaataagcg tcgctttggg tgagatcatc
     5161 gagcgcgacg gcttcgtttt tgccgatttg ctgcccttcc gcgaatagcc cagttcggcc
     5221 ccgttatggc cgaagttttc cggccgcgcg ggccggtggg cttgtgctaa agaagattaa
     5281 gtgccggcgg gccggccatg acgacttttt taacggcttt gaatgggcgt tatgaaacaa
     5341 atcggggcct cgcaacactt agttgtggag ttgctgaggc aggtgcttta tgaccgggtc
     5401 acaaagttac ggggcggcag acgcccgggc cgtctaccgg ctcgcggccg ctatcctggt
     5461 acaagccatt gaagacatac gaagcgggtc gcataacaaa agagccgatg ccgtccgctg
     5521 ggtcacagac cccacagacc ggcagttcag cttcaccttc tgttgccgcg tgctcagccg
     5581 caatcccgag caggtgcgcc gatttcttct ggcgcaagtc ccgcctgccc gatggagtct
     5641 ccggatagac gcacctcggt tttccgttta aacccgctcg gttgctcccg ggcattcccg
     5701 cgggcgttcg atccccgctg cttgcgaaca gggccgtgta cattgccccc gagctccaga
     5761 aatgctcacg gagagcaggc gcctgcggga tagattagt
//
LOCUS       DASREK010000197         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1420197, whole genome shotgun sequence.
ACCESSION   DASREK010000197 DASREK010000000
VERSION     DASREK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420197"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFA54_10240"
     CDS             complement(<1..359)
                     /locus_tag="VFA54_10240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS51230.1"
                     /translation="MKVTILSVAYPLAPVSLDAAGGAEQVLAMLDAALAQRGHRSVVV
                     APEGSAVQGVLMPTAAAKGELDEQARKRAWREHRDAIACALSRWPVDLVHMHGVDFYR
                     YLPAPGPAVLATLHLPLA"
     gene            complement(356..1180)
                     /locus_tag="VFA54_10245"
     CDS             complement(356..1180)
                     /locus_tag="VFA54_10245"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZS51231.1"
                     /translation="MRSEELLQRLCAADESTPGPKAMLVAAHPDDEVIGAGGRLRYLR
                     NATFVHITDGAPRNMRDARIAGFSTRQEYALVRRAELEMALSLAGFRPSDACSLSFVD
                     QEASFNLAELAHRLADLIRKFNPEIVLTHPYEGGHPDHDAAAFGVQLAHRLLEEQGFS
                     APAIVEFTSYHIRNGELETVQFLPGQPRGIAVLLSTADRKLKRQMIEHFITQQELLRS
                     FPVDIERFRVAPHYDFTRPPHEGRLHYENYDWGMTGERWRSLAQNALLELGLKVEA"
     gene            complement(1170..2057)
                     /locus_tag="VFA54_10250"
     CDS             complement(1170..2057)
                     /locus_tag="VFA54_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HZS51232.1"
                     /translation="MVEAVMLWNEPNNLSHWDFEVDPEWKTFASMVNIAADAIRAESP
                     RLRRVLGGISPIDPSFIATMSRHCVLHHVEAVAVHGFPLDWNHWTIHEWPARLAEIQA
                     VTPLPVWVSEAGVSSFGAEEVQDFGLRRTAELLIGRTERIHWYSLWDLPRAWPAATRH
                     REAEGSAYYRHFYMGLLREDGSPKLALRSFAEFTPALGICQWFHFEDHRLEDAARWLR
                     KLGVRHLRTGLSWADSLRPGADRWFDKQMKTLSGFDLTLTFCFTPECFGVRPHHTSPP
                     QKIERFGEFCARMTRRYAF"
     gene            complement(2050..2841)
                     /locus_tag="VFA54_10255"
     CDS             complement(2050..2841)
                     /locus_tag="VFA54_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HZS51233.1"
                     /translation="MISDVPGPLPGFTLGQIQQRVHDLGPWFHNLNLGGVQTAPDHFL
                     GDYPAVKWRRFSHALANDLRGKTVLDIGCNAGFYSFEMKRRGADRVLGIDSEEGYLDQ
                     ARFAAAVNGLDVEFRNLSVYEVAGLRERFDVVLFLGVLYHLRYPLLALDVIHEHAAKD
                     LLVFQSLLRGSPRVERIEKDYPFTETEVFNRPGFPCLHFVENCYAQDPTNWWIPNVAC
                     AQAMLRSAGFEILDRPEEEVFLCRRRNRAWRPRPEEELLMRRRRG"
     gene            complement(2838..>3032)
                     /locus_tag="VFA54_10260"
     CDS             complement(2838..>3032)
                     /locus_tag="VFA54_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51234.1"
                     /translation="VRRGRRDAYVGALVNEYLAQGGGASGVRAGTAYVDVGTLNGYRE
                     AVRLLSEPGHERVPAERRRL"
BASE COUNT          607 a          991 c          899 g          535 t
ORIGIN      
        1 gccagcggca gatgcagcgt tgcgaggaca gcgggacccg gcgccggcag ataccgataa
       61 aaatccaccc cgtgcatatg gaccaggtcc acgggccacc tcgaaagggc gcacgcgata
      121 gcatcccgat gctcccgcca agcccgcttc cgagcctgct catccagttc gcctttggca
      181 gccgcggtcg gcatcagaac gccttgcacg gccgacccct cgggcgccac cacgaccgac
      241 ctgtggcctc tctgcgccag cgcggcatca agcatcgcca gcacctgttc ggctccgccg
      301 gcggcgtcga ggctcaccgg cgccagcggg taggcgacgc tgaggatcgt caccttcatg
      361 cctcgacttt caatcccagc tccagcagcg cattctgagc gagcgatcgc cagcgctcgc
      421 cggtcatgcc ccaatcgtag ttttcatagt gcaatcgccc ttcgtgcggc ggcctggtga
      481 aatcatagtg cggtgcgacg cgaaagcgtt cgatgtccac ggggaaggaa cgcagcagct
      541 cctgctgggt gatgaagtgt tcaatcattt gccgcttcag cttgcgatcg gccgtggaga
      601 gcaaaaccgc aattccccgc ggctgtccgg gcagaaattg aaccgtctcg agctcgccat
      661 tccgaatgtg ataggaagtg aactcaacaa tggccggcgc ggaaaagccc tgctcctcca
      721 aaagccggtg cgccaactgc actccgaacg cggcggcatc atgatccgga tggcccccct
      781 cataggggtg cgtcaggacg atctcaggat tgaactttcg tatcagatcc gccagcctgt
      841 gggccagctc cgcgagatta aacgatgctt cctgatctac aaagctcagc gagcaggcgt
      901 cgctgggacg aaatccggcc agcgacagcg ccatttccaa ctcagcccgc ctgaccagcg
      961 catattcctg tcgcgtggag aatccggcga tccttgcgtc gcgcatgttg cggggcgctc
     1021 catcggtgat gtggacgaaa gtggcgtttc gcaaatagcg caagcggccg cctgcgccga
     1081 ttacctcgtc gtcgggatgc gccgcgacga gcatggcttt ggggccgggc gtggattcgt
     1141 cggcggcgca caacctctgg agaagctctt cagaacgcat accggcgcgt catcctggcg
     1201 caaaattccc cgaagcgctc gatcttttgg ggcgggctcg tgtggtgggg gcgcaccccg
     1261 aagcactccg gagtaaagca gaaggttaag gtcaaatcga agccgctgag cgtcttcatc
     1321 tgtttgtcaa accagcggtc ggcgcccggg cgaagactat ccgcccagct caggcctgtg
     1381 cggagatggc ggacgcccag cttgcgcagc caacgcgcgg cgtcttccag ccggtgatct
     1441 tcaaagtgga accactggca gatgcccagc gcgggcgtaa attccgcaaa gctccgcaag
     1501 gccagtttcg gcgagccgtc ttcgcgcagc agccccatat agaaatgccg gtagtacgcg
     1561 gacccctcag cctcgcgatg gcgcgtggcg gccggccagg cgcggggcaa atcccaaagg
     1621 ctgtaccagt gaatccgttc ggttcgccct attaacaact ccgctgtgcg gcgcaggccg
     1681 aaatcctgca cttcctcggc gccgaagctg gaaacgccgg cttccgaaac ccataccgga
     1741 agcggcgtga cggcttgaat ttcggccaat ctcgcgggcc attcgtgaat ggtccagtga
     1801 ttccagtcaa gcgggaatcc gtgcacagcc accgcttcca catggtggag cacgcaatgc
     1861 cggctcatag tcgcgatgaa actcgggtcg atgggcgaga tcccgcccag cacgcgccgc
     1921 agccggggac tttccgcgcg aatggcgtcg gctgcgatgt tcaccataga ggcgaacgtc
     1981 ttccactccg ggtcaacttc gaaatcccag tgcgatagat tattcggctc gttccagagc
     2041 attacggctt caaccacgtc ggcgcctcat caagagctcc tcttccggcc gggggcgcca
     2101 ggcgcgattc cggcggcggc agaggaatac ctcctcctcg ggacgatcga ggatctcgaa
     2161 gccggcgctg cgcagcatcg cctgcgcgca cgctacattg ggaatccacc agtttgtcgg
     2221 atcttgggcg tagcagtttt cgacgaagtg caggcacgga aatccgggac gattgaagac
     2281 ctcggtttcc gtaaacggat agtccttctc aatccgctct acgcgcgggc tgccgcgcag
     2341 cagcgattga aaaaccagca aatccttggc tgcgtgctcg tgaatcacat ccaacgccag
     2401 cagcgggtag cgcaggtggt acaggacccc caaaaacagc acgacgtcaa accgctcccg
     2461 caagccggcg acctcgtaca ccgaaagatt ccggaactcc acgtccaggc cgttcaccgc
     2521 agcagcaaag cgagcctgat cgagatagcc ttcttcggaa tcgatgccga gcacgcggtc
     2581 cgctcccctc cgtttcattt caaaagagta gaagccggca ttacagccga tatcgagcac
     2641 ggttttaccg cgcaaatcgt ttgccagggc atgggaaaac cggcgccact tcaccgccgg
     2701 ataatcgccc aggaagtgat caggagcggt ctgaacgcct cccaggttca gattgtgaaa
     2761 ccagggcccg aggtcgtgga cccgctgctg gatctggccg agagtgaatc cgggaagtgg
     2821 acccggcaca tcggaaatca tagtcgccgc ctctccgccg gcactctttc gtggccgggt
     2881 tcgctcagca gccgcaccgc ctcccggtaa ccgttcagcg tccccacatc cacataggct
     2941 gttccggcgc gcactccgct tgctccgccg ccttgcgcca gatactcgtt tacgagggcg
     3001 cccacatacg cgtcgcggcg cccgcgccgt ac
//
LOCUS       DASREK010000198        13855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_923113, whole genome shotgun sequence.
ACCESSION   DASREK010000198 DASREK010000000
VERSION     DASREK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923113"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VFA54_10265"
     CDS             <1..1250
                     /locus_tag="VFA54_10265"
                     /inference="COORDINATES: protein motif:HMM:NF020121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HZS51235.1"
                     /translation="YRFSDGFVLGEFQAGFGTVGLRVGAPVTSGDLTNWAWSALARGA
                     KGLNFYAFYPMSSGYESGGFGLIHLDGRITERAKVLGSIAKTVSAHGELFLKARPPKA
                     QVAILYNPLAYMVGGPRRLPAAGAQDEYEGVERDSWMGMYRALFPENVPIDYVHADDI
                     AENGISGYKLLYVPYPIMMSEGTARAIMRFIQEGGCVAAEARTAWNDARGYATATIPG
                     FGLDKIFGVREIVVTPVQRTALIVKSKNAAIPLLQPEARLPGFIYQEALEVEGPGQVL
                     AAFEDGSPAIVASTHGKGKTVYVGSYLSLAYERTRDVQLRRFFQGLLDWAGVERPVSA
                     SPGVEVRYLEGPGYTLDFILNDGEKDLPAEVRLRPPFPTPSVRNLVTGEAVSYRQEGS
                     RLVLTKTLPAQGAWVLEIRERGR"
     gene            1253..4519
                     /locus_tag="VFA54_10270"
     CDS             1253..4519
                     /locus_tag="VFA54_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS51236.1"
                     /translation="MRSKTLWWRRRAPMAAVLFCLQTLAIAQVNTASIRGTVTDSSGA
                     VVANASISATNDDTGAKFTTVSNARGIYVFPVLSVGAYTVTAELQGFKTAVRRGVELE
                     VNQTARIDLVLEVGSLAERVVVEAPAPLVETETSSLGTVVSQRDIVDLPLNKRNFSQL
                     VTLVPGANFGATGTIGGGDRPDDPRPRSSFFVNGTRDSSNTYLIDGIDNYDRIHTTIA
                     IKPSIDAVQEFKVQTSLYSAEFGRNAGGVVNVSVKSGTNQFRGSAYEFLRNQALDARD
                     FFSPPTQPKPHYILNQFGGTLGGPILKDKTFFFVSYEGFRERRGQTFTSTVPTLAMRN
                     GDFSGLGTIYDPLTNTPSGSSFSRTPFPNNRIPLDRMDVPAMRLIALYPQPTSSGLSN
                     NFVWSPVREQNTNQVDARIDQQIGNRGTLFGRYSFGDTDTITPALIPGKGQGGGNFSG
                     PNMLRTQGLALGYTDTISPTLVSETRAGFTRISSHVFPFFFGEKISDEVGIPNANQDQ
                     FSSGLTTISISGFRGLGNSSFQPIYKVENTYQVTENIHLTRGRHILKFGTQLIRPQAN
                     HFQSANPAGLFSFNANFTNNPLSPAGTGNAMATFLLGYPTSTSRTAQLSPNYIRSLES
                     ASYVQDDWKILPRLTLNLGLRYELVTAPVSANDHMTNFDFRQGKVILAGVNGVSRTAG
                     VKTDTNNFAPRFGFAYSLTQKTVVRGGYGVFYDAVPAFAELRPFPYLITYTVVPSAFQ
                     VQNRMSDGFPNADFDIAKNAANPFGSINAVPFDNPLGYIQQFNLNVQHSLAQNLGLTV
                     AYVGTLGRKLRWVYDEDIPDPGPGAVQQRRPFYSVAPNVTGLLTNHAEASSTYHSLQA
                     GLEKRLSSGLAFQASYTFSHWIDNAISEAGYGSQGPNPQDLRNRRAERGNDPADIRHR
                     FVYSWIYELPLGNGKRWAHEGVWLPRILGGWQINGITTLQSGLPFSVVLATPVTNTGT
                     GNRADRIGQGSLSRDQRTLSRFFDTAAFTTPAQFQYGNSGRDILFGPGMVNFDFSLFR
                     NIRFTETRSLQFRSEFFNLFNTPQFDLPNATLGSSAYGSISALVNSMRQMQFSLKLLF
                     "
     gene            4534..5499
                     /locus_tag="VFA54_10275"
     CDS             4534..5499
                     /locus_tag="VFA54_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZS51237.1"
                     /translation="MEQTGYAVGVDVGGTKVAAAVIDPRKVIHSKIRLSTEKQNRERS
                     IAQVAEAARKAVEAARVSWENIAAVGVDVPGVYYPGSGEVWAPNLPGWDRIPLRGELQ
                     TQLPRPVVIDSDRSAYVLGEQWAGIARGLTDVVFIAVGTGIGAGILSGGRLCRGAGGV
                     AGAIGWLAVDPNQKPLYRQTGCLEAEAAGPGVARRAAALAARVPSQMLEIAGGCLDNI
                     TAETVVEAARRNDAPARQVLEETARYLAMGVANVISMLNPQMIVLGGGLMQAGNLLLD
                     PIRRETVRWAQPLAAREVRIELTQLGEDAGLLGAARLALGHDHVG"
     gene            5489..6241
                     /locus_tag="VFA54_10280"
     CDS             5489..6241
                     /locus_tag="VFA54_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZS51238.1"
                     /translation="MSAEKYYERIQDVLALIRSTQMEKICQAGAVMAAAIGSGKRVYV
                     FGSGHSVLPVLDIFPRYGSFVGFFPLSDPRLMWFNVVGPGGARELLWLERREGYAEVF
                     LQSYPLAPGDCMVVFSHGGRNAAPIEVALTAKKKGLTVITVSSLANARAGKATHSTGK
                     QLPDAADIAIDNCVPPEDALVDVGWTERVAAGSTVAAVSIAMALVAETGAQLASRNCR
                     PPTFVSPNVAGTSANHNEKVFEAFTQQLFSRR"
     gene            6249..7433
                     /locus_tag="VFA54_10285"
     CDS             6249..7433
                     /locus_tag="VFA54_10285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51239.1"
                     /translation="MSAIRKDWLAGGAYAGMFVFGIVMALLGAVLPLISQQIGFDLAR
                     AGNLFLVMNCAMLASMLSLGLLIDRFGQKPILVGGSLCVAAALALLAGAAGYGTVLGS
                     AGLLGAGGGALNGATNTLISDLHTDPRRKSSALNLLGVFFGFGALFLPFTIGALLQAL
                     GLLPILYAAAAISAAPAALFLALAFPPAKQSQGIPLRKAAGLASHPLVLLIGFLLFFQ
                     SGNEFILGGYTSTYLTREVGISISAASYLLAAYWGAIMLTRFASSRLLLWMSGPALVL
                     RSALVSALGASLLVAAHSPSAAAAAVVLIGIGFASIYPTTLGLAGSCFESHSGTVFGI
                     IFAIALVGGMILPWAVGQIAAASALRWALVTAIVNSLLIVVLQLAVRKTAAKQGREQQ
                     SP"
     gene            complement(7621..9060)
                     /locus_tag="VFA54_10290"
     CDS             complement(7621..9060)
                     /locus_tag="VFA54_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HZS51240.1"
                     /translation="MKVVLISTYEMGRQPFGLASPAAWLKRAGAEVSCCDVSRQPLDE
                     AAVQAADLVAFYIPMHTATRLAVRLAPRVRSLNPRAHLCFYGLYAPVNEEFLRELGAG
                     TILGGEFEPELVALAERLEAGRAGPQQEPRISLERFHFVTPDRSGLPPPGEYARLIVP
                     GRGRRIAGYTEASRGCKHFCRHCPIVPIYGGHFRVVDREVVLEDIRRQVAAGARHITF
                     GDPDFFNGIGHSVAIVRRLHEEFPDLSYDVTIKIEHLLKHRGHLATLRDTGCLFVTSA
                     VESVDDRVLALLDKGHTRQDFLEAVRLCREASLTLAPTFVAFTPWTTLETYRALLACI
                     AELDLIPNVAPIQLAIRLLIPAGSRLLERPEVLSIIGEFDHAALTYRWIHPDPRMDEL
                     CSQIQSLVKRREAQRATRRAIFREIWRLAHQHSCPPHDGETGFRRRELQTELGGGKGP
                     EAARAIQPPDDRDRIDRAAIPYINEPWYC"
     gene            complement(9057..9929)
                     /locus_tag="VFA54_10295"
     CDS             complement(9057..9929)
                     /locus_tag="VFA54_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZS51241.1"
                     /translation="MHSLVLYNGRIIPATEKVLSPGQVGLLAGWGVFTTLRIYDGVPF
                     EFERHWARMSKDARLLHVPLAADPEQVRRQLCELVRANHAENGAARMCVVRNTGSFWE
                     GPGISTAADVIALTSDLKPWGEAVRLGVVPAARYAASPFSGTKILSWAWNLTWSEEAQ
                     QRGFDEALLLNERGEVAECTSANVFAVIDGKICTPPLSSGCLPGVTRSVLLEEIGGIE
                     ERSLRPEELYDASEVFITSTTRELLAVSEIEGHTISSGPVRGLLQQAFTDYVRQYTSK
                     FCSTAASPIVMQGQ"
     gene            9928..10374
                     /locus_tag="VFA54_10300"
     CDS             9928..10374
                     /locus_tag="VFA54_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZS51242.1"
                     /translation="MPFHHTMAAMTQVTVESVRNLQQRVVIREHILRADEPVEAGGND
                     TGPNPYEYLLAALGSCTSMTLLLYARRKGIALQKVRVELDHSRIHAQDCADCESKEGF
                     VTEIHRRIFLEGDLTAEQRARLLEIAARCPVHRTLTGEIKIRDRAL"
     gene            10512..13046
                     /locus_tag="VFA54_10305"
     CDS             10512..13046
                     /locus_tag="VFA54_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HZS51243.1"
                     /translation="MTSRNLLLTVVAAAGAAILAKETSIYPILKSTASIGKQKGDFYL
                     LPTNQLLRPWGEQARIAGRPVDLTFDSRQRILAVLDWRSVLLFDGSTGIRIAEIPSRP
                     TSYTGVAFRPGDRELWASETSRNGPDSILIVELDELGRPASQPRRIELPGHPVPAGIA
                     FSSDRATAYVAFSRNNTLAVVDARARQVKQEIPVGMAPFGVAVAEKAGKIFVSNSGGR
                     RPHAGDVLAPSSGSQVVSDPVTGAASSGSISVIDRETLSVRDVSVGLHPTALSLSPDS
                     KLLAVANGHSDSVTLIDTGSLAATEVKIPTWPNGNNTFLGSQPTAAAFAPDGKTIYVA
                     CGGNNAIAVLTGGKPWKVAGAIPTGWFPSAVGVDREGALRIVNIKGLGNTADGKGNFN
                     SKQYEGSLIKVPAPGAPQLAAGTREVAAANMPKFEPAGGVSNLSSLGIEYVWLIIKEN
                     RTYDQVLGDIGKGNSDPELVFYGRAVTPNHHALAERYVLLDNFYTGGAISFDGHQWLM
                     QAFVSDYVERAFAASPRGYAWNMSDALTISPAGFFWQSAPRPLSVRVYGEFCLPARWD
                     PATQNAVDMNESELRSGGEYWRLYKEGKWQTEVGCRSGVPALAPLVSQRYPNNSTSIP
                     DQIRADVYLQELAAFEKSGQMPHLTVLTLSSDHTNGTRPGSRTPRAMVADNDLALGRL
                     VEGISKSRFWPKSLILVVEDDAQDGLDHVDGHRTVALAIGPHVRRNVVDSNHYNHESM
                     VRTIQEIFRIPPRTRYLAAARAMTSIFTPEADTNPYQCLPARVPLDEMNPPLKALSGR
                     KLWAARQSLAMDWSEPDDIPQDTLNRILWWDSKGYDRPYPQGYAAK"
     gene            13252..>13855
                     /locus_tag="VFA54_10310"
     CDS             13252..>13855
                     /locus_tag="VFA54_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51244.1"
                     /translation="MLRAAITIAALLSCGARVAQAANVLVFGDDSRKNAAAAALTSLG
                     HTVTNVTALPGSLAGFQAVFSFQIFSALTGPQQSELEAFIQAGNGVYFTGERPCCDAA
                     NTSIGTILNALISGGGITVGNQGDVADSSYSFNPLAQGGITTTPNTLTSWSPASPGKL
                     SGVSGTHVLVSGNLGSVVGGVWGASDLSGGVGAIVILMDID"
BASE COUNT         2649 a         4333 c         4190 g         2683 t
ORIGIN      
        1 gctatcgatt ttcggatggc ttcgttctcg gggagttcca agccgggttt gggaccgtgg
       61 gcctgcgcgt gggcgccccg gtcacctccg gggatttgac caattgggcc tggagcgccc
      121 tcgcccgcgg cgccaaagga ctcaattttt acgcttttta tcccatgagc tcgggttatg
      181 aatccggagg ctttggattg attcatctgg acgggcggat caccgagcgc gccaaggtgc
      241 tcggctcaat cgccaaaacg gtcagtgcgc atggagagct gtttctcaaa gcgaggcccc
      301 cgaaagccca ggtcgccatt ttgtataacc cgctggccta catggtgggc ggcccgcggc
      361 ggctgccggc ggcgggagca caggacgaat acgaaggtgt cgagcgagat tcctggatgg
      421 gaatgtaccg cgctctgttc cccgaaaatg tgccgatcga ttatgtgcat gccgacgaca
      481 tagccgagaa cgggatttcc ggctacaagt tgctttacgt tccctatccc atcatgatga
      541 gtgaaggaac cgcacgggct atcatgcggt tcattcaaga aggcggctgc gtagcggctg
      601 aggcgcgcac ggcctggaat gacgcccgcg gctatgccac agccaccatt ccgggttttg
      661 gcctcgacaa aattttcggc gtccgcgaga tcgtagtaac gcccgtccaa cgcacggccc
      721 tgatcgtgaa gtcgaaaaac gccgccatac ctctcttgca gccggaggcg cgcctgcctg
      781 gcttcatcta tcaggaggcg ctggaggtgg aggggccggg ccaagtgctc gccgcctttg
      841 aggacggctc gccggccatc gtcgcttcga ctcacggcaa gggcaagacg gtctatgtgg
      901 gctcgtatct gagtctggcc tacgaacgaa ccagagacgt tcaactgagg cgtttctttc
      961 aaggtctgct ggattgggca ggcgtcgaac gcccggtctc ggcttctccc ggggtggaag
     1021 tccgatatct ggaagggccg ggttacaccc ttgacttcat tttgaatgac ggcgaaaaag
     1081 acttgccggc agaagtgcgg ctacggccgc cgttcccaac gccgtccgtg cggaatctgg
     1141 ttaccggcga agccgtatcg tatcggcagg agggatcgcg cctggtgctg acaaagactc
     1201 tgccggcgca gggggcttgg gtcctcgaga tccgcgaaag gggtcgctga gtatgagatc
     1261 aaaaacgctt tggtggcgcc gccgtgcgcc catggcggcg gttctgttct gtttgcaaac
     1321 tttggccatc gcccaggtaa acacagcctc gatccggggc acggtcacgg attcctccgg
     1381 cgctgtcgtt gccaacgcat cgatttcggc aactaacgac gacaccggcg cgaaatttac
     1441 gaccgtcagc aacgcacggg ggatttatgt attccctgtg ctgagtgtcg gcgcttatac
     1501 ggtgaccgcc gaattgcaag gtttcaaaac cgccgtgcgg cgcggcgtcg agttggaggt
     1561 gaaccagacg gcccgcatcg acctcgtgct cgaagtagga tcgctggcgg agcgcgtggt
     1621 ggtggaggcg ccggcgccgc ttgtagaaac cgaaacctcc tcgttgggga cggtcgtgtc
     1681 gcagagagat atcgtcgatt tgccgttgaa caagcgaaat ttctctcagc tcgtgacgtt
     1741 ggtgccgggc gccaactttg gcgcgaccgg aaccatcggc ggcggcgacc ggccggacga
     1801 cccgcggcct cgatcctctt tcttcgtcaa tggcacacgg gattcgagca atacctacct
     1861 gatcgacggc atcgacaact acgaccggat tcacactaca atcgccatca agccttccat
     1921 cgacgccgtg caggagttca aggtacagac gagcctttat tcggccgagt tcggccgcaa
     1981 cgccggcggc gtcgtcaatg tctctgtgaa atcgggcacg aaccagtttc gcggcagcgc
     2041 ttacgagttt ctgcgcaacc aggcgttgga tgccagagac tttttttcac cgcccaccca
     2101 gccgaaaccg cattacatcc tcaatcaatt cggcggcacc ctcggcgggc cgatattgaa
     2161 ggacaagacc ttctttttcg tcagctatga gggcttccgg gagcggagag gccagacttt
     2221 caccagcacc gtgccgactc tggccatgcg gaacggcgat ttctccggcc tggggacgat
     2281 ctacgacccg ctgacaaaca cgccaagcgg ttccagcttt agccgcactc ctttccccaa
     2341 caatcggatc ccgctggacc gcatggacgt cccggcaatg cgtctaatcg ctctttaccc
     2401 gcaaccaacc agcagcggct tgagcaacaa cttcgtgtgg agtccggtgc gcgaacaaaa
     2461 caccaaccag gtggacgcgc ggatagacca gcagatcggc aaccgcggta ctctgttcgg
     2521 gcggtactcg ttcggagata cagacaccat cacgccggcg ctgattcccg gcaagggcca
     2581 gggcggcggc aacttctccg gaccgaacat gctccgaact cagggcctgg cgctgggtta
     2641 caccgataca atctcgccga cgctggtcag tgagactcgc gccggcttca cccggatcag
     2701 ttcccatgtt tttccattct ttttcggcga aaagatttca gatgaggtgg gcattcccaa
     2761 cgccaatcaa gaccagtttt cttccgggct gaccaccatc tccatctccg gttttcgtgg
     2821 cttggggaac tccagctttc agccgattta taaggtcgag aacacgtacc aggtgacgga
     2881 aaacattcat ctcacgcgcg gccggcacat tctgaagttc ggcacgcagc tcatccggcc
     2941 ccaggccaat cacttccaga gcgccaatcc cgctggcctg ttcagtttca acgccaactt
     3001 cacgaataat ccgctgtcgc cggcaggcac gggaaacgcc atggcgacgt tcctgctcgg
     3061 ctacccgact tccacttcgc ggacggcgca gctctcgccg aactacatcc gcagcctcga
     3121 gagcgccagc tacgttcaag acgactggaa gattctgccc cgcctcacgc tgaacctggg
     3181 cctccggtat gagctggtca ccgcgccggt ttccgccaat gatcacatga ctaacttcga
     3241 tttccggcaa ggcaaagtca ttctggccgg agtcaacggc gtaagccgga cggctggcgt
     3301 taaaacagac accaataact tcgctccgcg gtttggcttc gcttactccc tcacgcagaa
     3361 gacggtcgtg cgcggaggct acggcgtctt ttacgacgca gtcccggcgt tcgccgaatt
     3421 gcgccccttc ccttacctga tcacgtacac cgtggtgccc agcgctttcc aagtgcagaa
     3481 ccgcatgagc gacggctttc ctaacgctga cttcgacatc gccaaaaacg ccgccaaccc
     3541 cttcgggtcc atcaatgccg tgccattcga caacccgctc ggatacatcc agcaattcaa
     3601 cctgaatgtc caacattctc tggcgcagaa cctgggtctg acagtggcct acgtcggaac
     3661 gctggggcgg aaactccgct gggtctacga tgaagacatt cccgatccag gaccgggcgc
     3721 cgtgcaacaa cggcggcctt tttacagcgt cgcgccgaac gtcacgggct tactgaccaa
     3781 ccacgccgaa gcgtcctcga cgtaccattc cctgcaagca ggcctggaaa agcgcctttc
     3841 gagcggcctc gcttttcaag ccagctacac gttctcccac tggatcgaca acgcaatttc
     3901 cgaggccggc tacggcagcc aggggcccaa tccccaggat ctgaggaacc gccgcgcgga
     3961 gcgcgggaac gatcccgctg atattcggca ccgcttcgtc tatagctgga tttacgaact
     4021 gccgctcggc aacggaaaac gttgggctca cgagggagtg tggctgccgc ggattctggg
     4081 aggatggcag atcaacggca ttactactct gcaaagcggc ttgcctttct cggtcgtgct
     4141 cgcgacgccg gtgaccaaca ccggaaccgg gaatcgggct gatcgcatcg gccaaggctc
     4201 tctctcccgc gaccagcgaa ccctgagccg cttcttcgac accgctgctt ttacaacgcc
     4261 cgcgcagttc cagtatggca attcgggacg cgacatcctg ttcggcccgg gcatggtcaa
     4321 ttttgacttt tcgctgttcc gcaacatccg gttcactgaa acgcggtcac tccagttccg
     4381 gagcgaattt ttcaacctgt tcaatacccc gcagttcgac ctcccgaacg caactctcgg
     4441 ctcttccgct tatggctcga tcagtgcgct ggtgaacagc atgcggcaga tgcagttctc
     4501 gttgaagctg ttattctgaa tccggcgagc cgcatggagc aaaccggata tgccgtgggc
     4561 gtcgatgtcg gaggcacgaa ggtggcggcc gccgtcatcg acccccggaa agtcattcac
     4621 agcaagataa gactatcgac ggaaaagcaa aaccgggagc gatcgatcgc gcaggtcgcc
     4681 gaagccgcgc gcaaggcggt tgaagcagcg cgcgtctctt gggaaaacat tgcagcagtg
     4741 ggcgtcgatg tccccggcgt gtactacccg ggcagcggcg aggtctgggc gcccaatttg
     4801 cccggctggg accgcatccc gctgcggggc gagttgcaga cgcagcttcc gcggcccgtg
     4861 gtgattgaca gcgaccgctc cgcgtacgtg cttggcgagc aatgggcggg aatcgcgcgc
     4921 ggcctcaccg acgtcgtttt tatagccgtg ggcactggca ttggagcggg aattctcagc
     4981 ggcgggcggc tctgccgcgg ggccggcggc gtcgccggcg cgatcgggtg gctggcggtg
     5041 gacccaaacc agaagccgct gtaccggcag acgggctgcc tggaggctga ggccgctggt
     5101 ccgggcgtcg ctcgccgggc cgcggctctc gcagcccgcg taccttcgca aatgctggag
     5161 atcgctggcg gctgtctcga caatatcacg gccgaaacag tggtcgaggc cgcccggcgc
     5221 aacgatgctc cggcccgcca ggtgctggag gaaacggctc gctatcttgc catgggcgta
     5281 gccaatgtga tcagcatgct gaatccgcag atgattgttc tcggcggagg cttgatgcag
     5341 gcaggcaacc tcttgctgga cccgatccgc cgcgagacgg tccggtgggc gcagccgctc
     5401 gccgcccgcg aggttcgcat cgagttgacg caattgggag aagacgccgg gctgctgggg
     5461 gcggcgcgcc tggcgctggg acacgatcat gtcggctgaa aaatactacg aacgaatcca
     5521 ggatgtcttg gctcttattc gctctacgca gatggaaaag atctgccagg ccggggccgt
     5581 tatggccgcc gctatcggct cggggaagcg cgtctatgtg ttcggcagcg gccactcggt
     5641 tttgccggtg ctcgacattt tcccgcgcta tggcagcttt gtcggcttct ttccgctatc
     5701 cgatccgcgt ctgatgtggt tcaacgtggt cggtcccggc ggcgcacggg agttgctgtg
     5761 gctcgagcgc agggaaggct acgcggaggt ttttctccaa agctatccgc tggcgcccgg
     5821 cgactgcatg gtggtctttt cacacggagg ccgcaacgcc gcgcccatcg aggtcgccct
     5881 tacagctaaa aagaagggct tgacggtgat caccgtttct tctttggcca atgcgcgcgc
     5941 ggggaaagcc acgcattcca cgggcaagca gcttccggac gccgcggaca tcgccatcga
     6001 taattgcgtc ccgccagagg atgcgctggt agacgtcggc tggaccgaac gtgtggccgc
     6061 cggttcgacc gtggccgctg tttccatcgc catggctctg gtcgcggaga ccggcgccca
     6121 actggcctcg cggaattgcc gcccgccgac gtttgtctcg cccaatgtcg ccgggacaag
     6181 cgcaaatcac aacgaaaaag tatttgaagc atttacgcag cagctcttca gccgccggtg
     6241 attcggccat gagcgcgatc cgcaaagact ggctggccgg aggagcctac gccggaatgt
     6301 tcgtctttgg gatcgttatg gccctgctgg gagccgttct ccctctgatc tcccagcaga
     6361 tcggctttga cctcgcgcgg gcgggcaatt tgttcctggt gatgaactgc gccatgctcg
     6421 ccagcatgtt gagcctgggg ctgttgatcg accgcttcgg ccagaagccc attctggtgg
     6481 gcggctcgct gtgcgtcgct gcggcgctgg ctctgctcgc cggggcggca ggctatggaa
     6541 cagtcctcgg cagcgctggt ttgctgggcg ccggcggcgg cgccctgaat ggcgcgacca
     6601 acactctgat ctccgatctg cacacggacc cgcgccgcaa gagctccgcc ctcaacctgc
     6661 tgggcgtatt tttcggtttc ggcgccctgt tccttccctt cacgatcggc gctctgctgc
     6721 aagccctggg tttgctccct atcctctacg cggcagccgc gatcagcgcc gccccggcgg
     6781 cgctgtttct ggcgctcgcc tttcctcccg ccaagcaaag ccagggcatc ccgctgagaa
     6841 aagccgcggg cctggcaagc catcctctcg tgctgctcat cggcttcctt ttattcttcc
     6901 agtccggcaa cgaattcatc ctgggcgggt atacctccac gtacttgacc cgcgaagtcg
     6961 gcatctccat ttcagccgcc tcctatctgc tggcagcgta ctggggggcg atcatgctga
     7021 cccgttttgc ttccagccgc cttctgttgt ggatgagcgg cccggccctg gtgctccgca
     7081 gcgccctcgt ctcagcgctc ggggcgagcc tcctggtagc cgctcattcg ccatcagcgg
     7141 cggccgcggc ggtggttctg atcggcatag gctttgccag tatctatccg acaaccctcg
     7201 gcctggcagg aagctgcttt gagtcccact ccggcaccgt cttcgggatc atctttgcga
     7261 tcgccctggt gggagggatg attctgcctt gggcggtcgg ccagattgcg gcggcatcgg
     7321 cgctgcgctg ggcgctggta acggcgatcg tcaattccct tttgattgtc gtgttgcaac
     7381 tggccgtgag gaagacggcg gccaaacagg gccgcgaaca gcaatccccg tgatgcgatt
     7441 tttctggtca gcgtatacgt caggatcaca gggcgtgttt gcgcaggcgc atttcaccgg
     7501 gcgattctgc cggagctcgt gctggcgatc atccatcccc ccgcccgaca ggcgatcggg
     7561 aaaatgggtc tcgctccggt gcgcctaaag aagagcaacc tgctcctcgg taggctccgc
     7621 tcaacagtac cagggttcgt tgatgtaggg aatggccgcc cggtcgatcc ggtcgcggtc
     7681 gtccggcggc tgtatggctc gcgccgcctc gggacccttt ccgccgccca attctgtctg
     7741 gagttccctc ctccggaatc cggtctctcc gtcatgcggc ggacagctat gctggtgagc
     7801 caaacgccag atttcgcgga agatggccct acgcgtagcg cgttgcgcct ctctgcgctt
     7861 caccaggctc tgaatctggc tgcacaactc gtccatgcgg gggtcagggt gaatccagcg
     7921 ataggtgagg gcggcgtgat cgaattcgcc gatgatcgag agcacttctg gccgctcgag
     7981 cagccgcgac ccggcaggta taagcaggcg gatggccagt tgaatcggag ccacgttagg
     8041 gatcaaatcc agttcggcaa tgcaggccag cagggcgcga tacgtttcca gggttgtcca
     8101 tggggtaaag gctacgaagg tcggagcgag cgtaagggag gcctctcggc agagccgcac
     8161 ggcctccaga aaatcctgcc gggtatggcc tttgtcgagc agcgccagaa cccggtcgtc
     8221 aaccgactcc acggcgctgg tcacaaagag gcatccggtg tcccgcagcg tcgccaggtg
     8281 tccgcgatgt ttgagcaagt gctcgatctt gatcgtcacg tcgtagctga gatccggaaa
     8341 ctcctcgtgc agccgcctga caatggcgac cgaatggccg atgccgttga aaaagtcagg
     8401 atcgccgaag gttatgtgcc gggcgcccgc cgcgacctgg cgccggatat cctcgagcac
     8461 cacttcgcga tccactacgc ggaaatggcc gccgtaaatc ggaacaatgg ggcagtgacg
     8521 gcagaaatgc ttgcagccgc ggctggcctc ggtatagccg gcaatccggc ggccgcgccc
     8581 cggaacaatc aagcgggcat attcgcccgg cgggggtagc ccggagcggt caggagtcac
     8641 gaaatggaac cgctccagag aaatacgcgg ctcctgttgc ggcccggcgc ggccggcttc
     8701 caggcgctct gccagcgcga ccaactccgg ctcaaactcg cccccgagaa tcgttcccgc
     8761 tccgagctcc cgcaggaact cctcgttcac cggggcatag agtccgtaaa agcacaggtg
     8821 agcacgcggg ttgagactgc gaacgcgcgg cgccaggcgt acggccagac gagtcgccgt
     8881 atgcatcggt atgtagaacg cgacaaggtc cgccgcctgc acagcagcct catcgagagg
     8941 ctggcgagat acatcacaac aggagacttc tgccccggcg cgcttgagcc acgccgcggg
     9001 agaagccagg ccgaacggct gccggcccat ttcataggtc gaaatgagaa cgaccttcat
     9061 tgtccctgca ttacgatcgg tgaagcggca gtggaacaga atttgctcgt gtactggcga
     9121 acgtagtctg tgaaagcctg ttgcaaaagc ccgcgtacgg ggccggagga aatcgtatgc
     9181 ccttcgattt cggacacggc cagcagctcg cgcgtagtgg aggtgataaa cacttcgctt
     9241 gcgtcgtata gctcttccgg gcgcaaagaa cgctcttcga tgcccccgat ctcctccaga
     9301 agcacggacc gcgtaacccc cggcagacag ccggacgaca gcggcggcgt gcagatcttc
     9361 ccatcaataa ccgcaaacac gtttgccgac gtgcattcgg ccacctcgcc gcgttcgttc
     9421 agcagaagtg cttcatcgaa gccgcgctgc tgggcctctt ccgaccaggt gagattccac
     9481 gcccaggaaa ggattttggt cccggaaaag ggggatgccg cgtagcgggc cgccgggacc
     9541 acgcccaaac gcaccgcctc gccccagggt ttcaagtccg aagtcagggc gatcacgtcg
     9601 gcggcagtcg aaattcccgg gccttcccag aagctgcccg tattccgtac cacgcacatc
     9661 cgggccgccc cgttctcggc gtggttggcg cggaccagct cacagagctg ccggcgcacc
     9721 tgctcggggt ctgcggctag cggaacgtgc agcaatctcg cgtctttcga catgcgcgcc
     9781 cagtggcgct caaattcgaa cgggacgccg tcatagattc gcagcgtcgt gaatacaccc
     9841 caacccgcca gcagcccgac ctgcccgggc gacaatacct tctcggttgc aggaatgatc
     9901 cggccgttgt acaggacgag ggaatgcatg ccttttcatc atacaatggc ggccatgact
     9961 caggttactg tggaatccgt acgtaacttg cagcagcgcg tcgtcatccg cgagcacatt
    10021 ttgcgcgccg acgagccggt agaagccggc ggcaacgaca ccgggcccaa tccctacgaa
    10081 taccttctgg ccgctttagg cagttgcact tcgatgacgc tgctgctcta cgcccgccgc
    10141 aaaggaatag cgctccaaaa agtgcgggtc gagcttgatc attcccgcat ccacgcccag
    10201 gattgcgccg attgcgaatc gaaggaaggt ttcgtaactg aaattcatcg gcgtatattt
    10261 ctggaaggag atctcactgc ggagcaacgc gctcgcctgc tcgagatcgc cgcccgttgc
    10321 ccggtgcatc gcacgctgac cggagaaatc aaaattcgcg accgggcgct gtgaaaaacc
    10381 ggaaaacgaa agttacagct tggataacgc ctatgctgcc gctcggcagc aggtgaaaaa
    10441 atcgtcgatt ttcaggcaga cccttgcgta ttccggttga gaatccggaa gcggtgcagt
    10501 aggctgtggg catgacctcc agaaatcttc tcctcacggt cgtggcggcc gccggcgccg
    10561 ccattttggc aaaggaaacg agtatttacc cgatcctgaa aagcacagcc tcgattggga
    10621 agcaaaaggg cgacttctat ctcctcccga cgaatcagct tttgcggccc tggggcgaac
    10681 aggcgcgcat cgcgggacgg cccgtcgatc ttacgttcga ctcccgccag cgaattctgg
    10741 ccgtgctcga ttggagaagc gtcctgctgt ttgacggctc tacgggaatc cggatcgccg
    10801 aaatcccttc gcggccgact tcttacaccg gcgtggcctt tcgtcccggg gatcgcgagc
    10861 tgtgggccag cgagacctct cgcaacggcc ccgacagcat tctcatcgtg gagttggatg
    10921 agctcggcag gcccgcttcc cagcctcgtc gaatcgagct gccagggcat cccgttccgg
    10981 ccggcatcgc cttttccagc gatcgcgcga cagcctacgt cgctttcagc cggaacaaca
    11041 ccctcgccgt ggtcgacgcc cgcgcacggc aggtgaagca ggagattcct gtaggcatgg
    11101 cgccgttcgg ggtcgcggtc gccgaaaagg cgggaaagat ttttgtttcc aattctgggg
    11161 gccggcgccc gcacgccggt gatgtactgg cgccttccag cggatcccaa gtggtaagcg
    11221 atccggtgac cggagcagcc agttcgggct cgatcagcgt catcgatcgc gaaaccttat
    11281 cggtaaggga tgtctccgtc ggcttgcatc ctacagcgct ctccctgagt cccgatagca
    11341 agctgctggc cgtggcgaac gggcactctg attcagtgac cctaatagac acggggagcc
    11401 tagctgcaac agaagtaaag atccccacct ggcccaacgg caacaacaca tttcttggca
    11461 gccagccgac cgcggccgct ttcgcgccgg acggcaagac gatttacgtc gcgtgcggcg
    11521 gcaacaacgc catcgcagtc ttgaccggag gcaaaccatg gaaggtcgcc ggggcgatcc
    11581 ctaccggctg gttcccttcg gcagtgggcg tcgaccggga aggagctttg cgtatcgtca
    11641 atatcaaagg gttgggcaat accgccgacg ggaaaggaaa tttcaattcg aagcagtacg
    11701 aaggatcgct gattaaggtc cccgcgcccg gcgctcccca gcttgccgcc ggcacgcggg
    11761 aagtagccgc agcaaatatg ccgaaattcg agccggccgg cggcgtctcg aatctttcct
    11821 ccctgggcat cgaatatgtg tggctgatta tcaaagaaaa ccgcacctac gatcaggtcc
    11881 tcggcgacat tggaaaagga aacagcgatc cggagctcgt tttttacggc cgggcggtga
    11941 ctccgaatca tcatgccctg gccgagcgat acgtgctgct cgacaacttc tacaccggcg
    12001 gagcgatcag cttcgatggc catcaatggc tgatgcaggc gttcgtgagc gactatgtcg
    12061 aacgggcatt cgccgcctcg ccgcgcgggt atgcctggaa catgagcgat gctttgacaa
    12121 tctccccggc cggttttttc tggcaaagtg cgccccggcc gctcagcgtt cgcgtctacg
    12181 gcgagttttg cctgccggcg cgatgggatc ccgcgacgca gaacgcagtt gacatgaatg
    12241 agagcgagtt gcggagcgga ggcgaatact ggcgcctgta caaggagggc aagtggcaaa
    12301 ccgaggtcgg atgccgcagc ggcgtgcccg cgctggcgcc tctggtcagc cagcgctatc
    12361 cgaataattc aaccagcatt cccgaccaga tccgcgccga cgtgtatttg caggaactcg
    12421 ccgctttcga aaaaagcggc cagatgccgc atctcaccgt actcacattg agcagcgacc
    12481 ataccaacgg aacgcgcccc ggctcgcgga cgccgcgcgc gatggtcgcc gacaacgatc
    12541 tggccctggg gcgcctcgtg gagggcattt caaagagccg tttctggccc aaaagcctga
    12601 tcctggtagt tgaggatgac gcgcaggacg gtctggatca cgtggatggc catcgaaccg
    12661 tagcgctcgc catcggaccg catgttcgcc gcaacgtcgt ggactcgaat cattacaacc
    12721 acgaaagcat ggttcgcacc atacaggaaa tcttccggat tccgccccgc acccggtacc
    12781 tggccgccgc gcgggccatg accagcatct tcactccgga agcggacacc aatccgtatc
    12841 agtgcttgcc ggcgcgcgtt ccgctggacg aaatgaatcc ccctttgaag gctctcagcg
    12901 gccggaagct atgggccgcc cgccaatcgc tcgccatgga ttggtccgaa cccgacgaca
    12961 tcccccagga caccctgaac cgcatcctct ggtgggacag caagggctat gaccgcccat
    13021 acccgcaggg gtacgccgcc aagtaatggc ccggcccgca gttttctttg taaaaccatc
    13081 ctttggtgag atatattaca ttcagcaaag cgggtgagcg acagcaatag ccctcctctt
    13141 tgcgcaaatc gaagcgcggc gctgcttctt ccgcagtgcc gcccaccgga ggaaggatct
    13201 atgtatatcg ctgtctcccg aagaacggct tggcgaacgc gatcatccgc tttgctgcga
    13261 gccgcgatta ccattgccgc gctgctaagc tgcggcgctc gagttgcaca ggcggcgaac
    13321 gtcctcgttt ttggagatga cagccgcaag aacgcggctg ccgccgcact gacctctctg
    13381 gggcacacag tgactaacgt tacggcgctc cccggcagct tggcaggctt ccaggccgtc
    13441 ttctcgtttc agattttcag cgcgctcaca ggtccacaac agtccgagtt ggaggctttc
    13501 atccaggccg gcaatggcgt gtacttcaca ggggagcggc cctgctgcga cgcggccaac
    13561 acatccattg gaactatttt gaatgcgctg atcagcgggg gcggcataac ggtggggaat
    13621 cagggcgacg tcgccgactc tagttactcg ttcaatcctt tggcgcaagg cggcatcacc
    13681 acgaccccca atacgctcac ctcctggagc cccgcgtccc caggaaagct ttccggcgtc
    13741 agcggcacgc atgtgctcgt ctcaggcaat ctgggttcgg tggtcggcgg ggtgtggggc
    13801 gcctccgact tgagcggagg cgtcggcgcg attgtcattt tgatggatat tgact
//
LOCUS       DASREK010000199        17169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_923288, whole genome shotgun sequence.
ACCESSION   DASREK010000199 DASREK010000000
VERSION     DASREK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17169
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923288"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFA54_10315"
     CDS             <1..664
                     /locus_tag="VFA54_10315"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZS51245.1"
                     /translation="GAHVVQIPTIEIQPAADWKPLDAAIARLESFDWLIFTSSNGVRF
                     FIQRLDASQRDLRGLRARLCAIGPATAAALAALHLKIDLMPAEYVAESVVAAFEKIPL
                     EGARILLPRAAVARDLIPTELEKRGARVQVVEAYRTAPPREAKWPEGLHPDWIVFTSS
                     STVENFHALFGAARLEGVRVASIGPVTSATARRLGIPVTIEPKEHTIPGLVEALAAAE
                     DP"
     gene            complement(674..2656)
                     /locus_tag="VFA54_10320"
     CDS             complement(674..2656)
                     /locus_tag="VFA54_10320"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HZS51246.1"
                     /translation="MPDNFETLVNSGDESIYHVQSNAPGPKGSLPLTAQMLIARPSGD
                     IFGLSMDAGMGWTPSELGRKEFLILSTQGGIRAADGTPVALGYHTGHWEVGLLMQAAA
                     EELKALGAIPFAAFCSDPCDGRTQGTVGMYDSLAYRNDAAQVFRRLIRSLPTRKGVMG
                     VATCDKGLPAMMMALAAMYDLPTVLVPGGVTLPPTSGEDAGKIQSIGARFAHGQISLE
                     EAAEVACHTCATPGGGCQFLGTAATSQVVGEALGLELPHSALAPSGQPVWLDMARRSA
                     RALLELEARGISTGHILTEAAIHNAMAVHAAFGGSTNLLLHIPAIAYSAGIRRPTIED
                     WIRFNRRVPRIVDVLPNGPKMHPTVRVFLAGGVPEVMLHLRRLKMLEESALTVSGEPL
                     GRVLDWWEKSERRRRMRELLHRLDGVRPEEVILDPETAQREGLTSTVTFPRGNLAPEG
                     SVIKSTAIDPSVVDADGVYRKTGPARVFTRERDAIRAIKGHGPDAIKPGDVLVLICRG
                     PQGAGMEEIYQITAALRHLSFGKHVAVVTDARFSGVSTGACIGHVSPEALAGGPVGKV
                     REGDLIRIWIDRNKLEGSIDLVGADGCEFGPEEGERVLAQRPPRPDLAPDSELPPDTR
                     LWAALQQVGGGVWGGCVFDVEAIVKTLEAGRKALAR"
     gene            2904..5447
                     /locus_tag="VFA54_10325"
     CDS             2904..5447
                     /locus_tag="VFA54_10325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS51247.1"
                     /translation="MRRSLKHWIGRVLGKSGPPVVAVFLSGDAALSRSMALRMRELAP
                     DYPHVIIGAGEIDRSHFKYAQDIIVVNPHRLLASWLELRRRLGRRWIALAPFLWQRGE
                     QSEADSYRLLRWLPWLLAPRKLLAFNARLERHHVRLRLPIASWRFLRGEPVGDILRPT
                     PLGHLKKLRALAGFPLVAACWAAVQMRRRPPAPLPPASQARPGVTALALPEKIDGAVC
                     AELDRSVCGADTEHILIITGGRELPPATRLRLEALLERPHTWLAYTGRKISVAAGVPV
                     DAPVKTGEDGTWALCSDPAAALFRRDVYLALGGMASLEGAYGGAAWLALSLLGWQRGW
                     HTLFAGAAEAPIGRDFAQERLMLGAICDFRTAIRILWEWSGDWRRWKGALSALGGIAT
                     PAPASAAALDPGARVSGSCRDFWPLLDPRVHVFQGRRPSGRQQVAVVSPYLPFPLSHG
                     GAIRIFNLTRTAGGHADISLFAFAEKETGREIEPLLHSCTRVVLVETPRWEPAGLFRL
                     WPRGVCKFRSRIMRAAISRLVRDEQISVVQIEYTQLAHLRSAAAAPAGTVLVEHDVTF
                     DLYRQLRIRAQGWEKVKARLEELRWKRFELGFARAFDRVIAMSQEERARLLRGGLPPE
                     RVAVVENGVDLERFRPLAPEPAALPELLFIGSFRHFPNVLGFEFLVRQVWPVLRQTNP
                     ELTLTVVAGADHEYYWERHTNTKWRETAGIDVLGFVEDVRPLYRRATVVAIPLLVSAG
                     TNIKVLEALAMGRPVVSTLVGVAGLGLVTGENVLIADTAQEFAEAVSRLLGDALLRAA
                     IARRGREFVEERYGWDALGEKLAGLWADPNLKAPEASAMSSKAYRPARA"
     gene            complement(5389..6390)
                     /locus_tag="VFA54_10330"
     CDS             complement(5389..6390)
                     /locus_tag="VFA54_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HZS51248.1"
                     /translation="MEDTSRQWAPPAAAYGAIAVFFAFSARMELRRYPVAAVPAASIY
                     ACLLLLFALLLAPGFRVSRRWLAARLHGARPAALCVGLFLAPYLVYASGVGDFRRMAF
                     LKLLLFVAVPFFLYELLPVRFPDRMNWQDLLVLIWLALPIFSGQIRGVWNVPVNLDFM
                     ARLLLVAVGAWSFLLWRGLDGSGYEFSFSLAVIRDAAVHFALFAAIAIPLGFAMRFIG
                     WNPRWRGLWAFSFDLATIFVFIAVAEELYFRGILQNLLERTWHSRLVAQATASLLFGL
                     SHIRHASFPNWRYVVLASIAGWFYGSAYQQRRSLMASATMHALVDTLWRTWLTLPGP"
     gene            6454..7335
                     /locus_tag="VFA54_10335"
     CDS             6454..7335
                     /locus_tag="VFA54_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HZS51249.1"
                     /translation="MRLAWLFLFWAGLSFGQLKGIIDLHAHCDPDSMPRSVDALELAR
                     LAQKEGMRALLLKNHFAPTAQLAYIVSHAVSGIQAYGGIVLNGPVGGINAAAIEQMAR
                     FKGGYGRVVWMPTFDAENQVRVSKENRPFVPVSRNGVLLPEVIRVIGIIAREKLALAT
                     GHSSPAEDLMLIREARRAGITRIIVTHAMFSPVSMSVEQQKEAAKLGAYIEFCYNALL
                     PAAGANRLQPADYVRAIHAVGAEHCVLSSDLGQANNPVHTEGWKMYLDTLRKAGITES
                     EIDWMARRNPARLLALE"
     gene            complement(7271..8482)
                     /locus_tag="VFA54_10340"
     CDS             complement(7271..8482)
                     /locus_tag="VFA54_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HZS51250.1"
                     /translation="MIQNGHFSRRHVLTAFLICGAILFAWSQKPPGRTSPGAFQGVYL
                     TSWSAGSPARIRRVIELAQAGRINTVVIDIKDATGYVAYATTAPDAARYGARRVTIRD
                     VDSLLGRLHREGLYVIARIVVFQDPRLALARPELAVHQHLEAPPAESDGAGAALRPAA
                     VLWLDRKHLAWIDPASREAWSYNAALAKEALSRGFDEINFDYVRFPSDGDLKQMEFPV
                     WDGATPKREVIRRFFAYVRREMPDAVLSADLFGLSTVNHDDLGIGQVIEDAYPFFDYV
                     CPMVYPSHFAAGFLGFPNPAEQPYRVVNYSLGSAGKRLRNARPVRGRLRPWLQDFNIG
                     ARYDKDRVEAQIKAVQDALGDAYAGYLLWSPSNNYTEEALAVIPRSSSPIPGPAAAPD
                     YAAPSSRSRSR"
     gene            8592..9671
                     /locus_tag="VFA54_10345"
     CDS             8592..9671
                     /locus_tag="VFA54_10345"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZS51251.1"
                     /translation="MRPILVLIALAGICLAAPKTETGELNGAQFRIDIPDRWNGGLIL
                     YCHGYNDTPVRYDGAKPNPLISIFTGQGYAVAQSGYAAGGWAIQEALEDTEALRRYFT
                     AKYGQPKETYVTGHSMGGFLTMELIETFPNSYDGGLALCGPLGAASWFMLRRVFDMRV
                     VFDYYFPGVLPSPVKVPADFSRTPQRVAEVLKALEEKPEPAAAMRRYSNIRTNKELAG
                     TIVFWTYILKDLQQRGGGNPFDNRNTIYDGTPDDNAVNSGVGRYKADAQAADYIRHYY
                     TPTGRLTRPLLAIHSTYDVLVPPWVTNQYSIIAAEAGSSDWFVQQYVKHDGHCAILPD
                     EIRRGFDQLREWKTIGKRPPAGENR"
     gene            complement(9758..10804)
                     /locus_tag="VFA54_10350"
     CDS             complement(9758..10804)
                     /locus_tag="VFA54_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZS51252.1"
                     /translation="MNRAPSLFVLLCLAGFLSGCGNSRTQERKESQGERIYVTNEGSG
                     DLSVIDAEKLEVIATVPLGKRPRGIHASPDRRTIYIALSGSPFAGPGVDESKLPPPDR
                     SADGIGVFDVQQNKLLKVMKSGNDPEQFALSRDGKLLYVSNEDAATASIFDLASEKVA
                     QALPVGSEPEGVTMTPDGKFVYVTSEGEGALFVIDARAGKVVKNFKVGHRARSVAFLP
                     DGSRAYVTCEVDNLVAVVDAVRQELIKTIPLGGKQFRPMGVVVSPDGSQVYVSSGGGH
                     RVFVISTATDQITGSIEVGQRPWGIALSGDGKKLYTANGPSNDVSVVDLAKQAVIKKI
                     KVGDRPYGVLVLSP"
     gene            10972..11937
                     /locus_tag="VFA54_10355"
     CDS             10972..11937
                     /locus_tag="VFA54_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZS51253.1"
                     /translation="MLNRREFLTTTALGIGAAAVAQALPSDKMRFAMSGHEFRKMQPH
                     PEAGIKVTARYGYHGLEPFQEDVAKYLDKPPEALKQVLEASGLALCTIGSGGQYQDPA
                     KIPQTIENNVARCRYIASFGCKHLKVNLNRRPGDGRANLSPEDAKALARTLNEIGKRT
                     MGEGGVKFAFHPHCWTIVEREPELRQIMDLTDPKFVYLIADTCHLSLGGIDPAKAVRD
                     YYSRLAAVHLKDCEPKYSPARGWKGPAPSEEEHNRVNLYKRLGTGGVDFPALFHLLRE
                     RDYDGWVTLDFDAPRPGEGTVEEDMNSHRKYLMETLHVTLRNGQG"
     gene            12035..12829
                     /locus_tag="VFA54_10360"
     CDS             12035..12829
                     /locus_tag="VFA54_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HZS51254.1"
                     /translation="MARVTRIASGKKVPAAAVRAGGKRMPSFAPFFCGVCAFVLLLAF
                     AGGAGRAAPSGPASETWIFDRIDRIGGHPTTVLGHPRVIDSPVGKAVEFNGVDDALFI
                     GVHPLAGAETFTWEAIFRPDGGAREQRWFHLQESGSDNRMLFEIRVTPEGQWFLDSFN
                     QTGAASKALINRNALHPTGAWYHVAAVYDGREFRNYVNGIQEGAAEIHLAPQGPGQTS
                     VGVRINKVNYFKGAVHLARFTRRALTPAEFLKLPPGAASGTGNGSR"
     gene            complement(12844..14643)
                     /locus_tag="VFA54_10365"
     CDS             complement(12844..14643)
                     /locus_tag="VFA54_10365"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZS51255.1"
                     /translation="MLQRRCWIVFLLGALAAAGCGRRKMPEAFASLAEEFVYSTLALS
                     PVSATATGYHVHKDPKTGAERKLDEMLDDLSPAGIDQQRQFYTGFRRRLSDAVDPAQL
                     EPEDRADFDIIQDQIALNLLELDEIQNYRHNPTLYVELIGNALFNPHVLEYAPKDVRV
                     RQIIERMKKIPSFLDQAKANLAAAPEIWTRVALEENEGNLNLIENIIRASLPEELRAE
                     YDSASKPAVAALRAFQDYLRNDLAKRTNADWRLGKERYQKKFRYVLETDLTPEQVLAS
                     AEADVKSVRARMYELALPLYQKMFPGKRDVKDETRVIGETLDHIARRHSTRESYIPDA
                     RKDLEEAREFVRAKDLLTLPPHENLLVIETPEFMRGIYGVGGFNAAPALEPQLGAFYW
                     ITPIPKEWPAQRVESKLREYNYYKLKLLTIHEAMPGHYVQLEYANQIQPKARRILRAV
                     FGNTPYIEGWGQYAEQLMLDEGFDSSPEMRLTFLKEELRVLANAILDIRLQTMGMTDQ
                     QAMDLMEKDTFQEHEEAAAKLQRAKLSSCQLPTYRVGWRDWLRVREQYNRVKNGGYRL
                     KDFNEQALREGAVPLPVLARLLTGKPLEQESAK"
     gene            complement(14666..16009)
                     /locus_tag="VFA54_10370"
     CDS             complement(14666..16009)
                     /locus_tag="VFA54_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZS51256.1"
                     /translation="MQETIFQIARWLLIGILAVWAARRRSLTAWILVSMAAGAEFGYD
                     WPGTAVKLQVLSLIFLRMIRAVIAPLLFATLVVGIAGHADLKQIGRMGIKALVYFEMV
                     TTIALFIGLAAINISRAGVGVRLPPSAIGIQASRHSTTALPAEKAPVQAARPPASDVI
                     LHVFPENIAQSVAEGQILQIVVFSIVFGVALAMLSERKRRPMLAFCESLSDTMFQFTN
                     IVMLFAPVGVGSAIAYTVGHMGLGILASLAKLLGTLYAALAAFLVLVLLPAAKIAGVP
                     IRAFARAVAEPASIAFATTSSEAALPRAMESMEAIGVPRPIVAFVIPTGYSFNLDGTT
                     LYLSLASMFVAQSAGMHLGFGQQVFMMLTLMLTSKGVAGVPRAALVILLATASSFHLP
                     MEPVLVLLGVDALMDMGRTSVNVIGNCLASAVVARWEGQFGVKTELPVIQESLAE"
     gene            16303..>17169
                     /locus_tag="VFA54_10375"
     CDS             16303..>17169
                     /locus_tag="VFA54_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZS51257.1"
                     /translation="MKIRFQPILGLAVIAWHLATALAASAQTGAKDGEWRTYGGDLGN
                     TRYSPLDQINASNFKDLEVAWRFKTDSLGPRPEFQFEATPLMVHGKLYSTAGSRRAVI
                     ALDAATGELLWMHSENEGARGEAAPRQLSGRGLAYWTDGKEERILYVTPGYRLIALNA
                     KTGVPIASFGKNGVVDLKQDDDQDIDLVTGEVGLHAAPIIAKNVVIVGAAHMSGSVPK
                     SKKNVKGYVRGFDVRTGKRLWIFHTIPRPGEFGIETWEKDSWSYTGNAGVWGQISVDE
                     DLGLAYLPVELPT"
BASE COUNT         3361 a         5293 c         5329 g         3186 t
ORIGIN      
        1 gggggcgcac gtcgtgcaaa tacccaccat tgagatccag cccgcagccg actggaagcc
       61 gctcgacgcg gctattgccc gtttggaaag cttcgactgg ctgatattca ccagctcgaa
      121 cggcgtgcgg ttctttatcc agcggttgga cgcctcgcag cgcgatcttc gcggattgcg
      181 cgcccgcctc tgcgccatcg gcccggccac ggcggccgcg ctcgccgccc tgcacttgaa
      241 aatcgatttg atgccggccg agtacgtggc cgaaagcgtg gtcgcggcgt ttgagaaaat
      301 cccgttggaa ggcgcgagaa tcctgctgcc gagggcggcc gtagcgcggg acctgatccc
      361 tacggagctc gaaaaacgcg gcgcccgggt gcaggtcgta gaagcctatc gtacagctcc
      421 gccgcgcgag gccaaatggc cggaaggact gcatccggat tggatcgtgt tcaccagttc
      481 gtcgaccgtg gagaatttcc atgctctgtt cggagccgcc cggctggaag gcgtgcgcgt
      541 ggcctcaatc ggccccgtga cgtccgcaac ggcgcggcgt ctcggaattc cggtcaccat
      601 cgaaccgaag gagcacacca ttcccggact ggtcgaggct ctcgccgcgg cggaagatcc
      661 gtagaactgg cggctaccgg gcaagcgcct tgcgtcccgc ctcgagcgtt ttcacaatcg
      721 cctctacgtc aaatacacag ccgccccaca cgcctccgcc cacctgctgg agcgccgccc
      781 acaagcgggt gtccggcggc aactccgaat ccggcgcaag atcggggcgc ggcggacgct
      841 gcgcgagcac ccgctccccc tcttccggac cgaattcgca gccgtccgcg cccaccaggt
      901 cgatgctccc ctcaagcttg ttgcggtcga tccaaatgcg aatgaggtcg ccttcgcgca
      961 ccttgccgac cggcccgcct gctagagcct cgggactcac atggccaatg caggccccgg
     1021 ttgagacgcc ggaaaagcgg gcgtcggtaa cgaccgcgac gtgcttgccg aaactcaaat
     1081 gccggagcgc agcggtgatc tgataaatct cttccatgcc ggcgccctgc gggccgcggc
     1141 aaatcagcac caaaacatct ccgggcttga tggcgtcggg cccgtggccc ttgatggccc
     1201 ggatcgcgtc gcgctcgcgg gtgaacacgc gcgctgggcc tgttttgcga tagaccccgt
     1261 cggcgtccac cacgctggga tcaatggccg tgcttttgat taccgagccc tccggggcca
     1321 gattgccgcg cggaaacgta actgtgctgg taaggccttc gcgctgcgcc gtctccggat
     1381 cgagaatcac ctcctcgggg cgaacgccgt cgaggcggtg caacagttcg cgcatacgcc
     1441 gccggcgttc cgatttctcc caccagtcga gaacccgtcc gagcggctcg ccggaaaccg
     1501 tcagcgctga ttcctccagc attttgagcc ggcgcaggtg cagcatcacc tcgggaacgc
     1561 ccccagccag gaatacgcga actgtgggat gcatcttggg gccgttcggc agaacatcca
     1621 cgatgcgcgg cacccggcga ttgaagcgga tccaatcctc gatcgtaggg cgcctgatgc
     1681 cggcggaata ggcgatggct gggatgtgca gcagcaggtt ggtcgagcct ccaaaggccg
     1741 catgtaccgc catggcattg tggatcgccg cttcggtgag aatatgcccg gtcgaaatgc
     1801 ccctcgcctc cagttccaga agagcgcggg cggaccggcg cgccatgtcg agccagaccg
     1861 gttggccgga aggagcgagc gccgagtgcg gcagctcgag gcccagcgct tcgcccacca
     1921 cttgcgaagt cgccgctgtt cccaagaact ggcagccccc gcccggcgtc gcgcaggtgt
     1981 ggcaggccac ttcggcggct tcctccaggg agatctgccc gtgagcgaac cgcgcgccga
     2041 tggattggat cttcccggca tcctcgccgg aagtcggagg cagcgttacg ccgcccggta
     2101 caagcaccgt gggcaaatcg tacattgcgg ccagagccat catcatggcc ggcaacccct
     2161 tgtcgcaggt cgctacgccc atgacgcctt tccgggtagg cagggagcgg atcaggcgcc
     2221 gaaacacctg cgccgcgtcg ttgcggtagg ccaggctgtc atacatccca accgttcctt
     2281 gggtccgccc atcgcagggg tcgctgcaaa acgcggcgaa ggggatggcc cctagcgcct
     2341 tcagttcttc cgcagccgcc tgcatcagca gcccgacctc ccagtggccc gtatgatagc
     2401 ccagcgcgac cggcgtacca tccgcggcgc gaatgccgcc ctgtgtactg aggatcagga
     2461 actctttgcg gccgagttcg gaaggcgtcc agcccatccc ggcgtccatg gagagaccga
     2521 agatatcgcc gctcggcctc gcgatcagca tttgggccgt caatggcagc gagcccttcg
     2581 gcccgggagc gttcgattgc acgtgataga tggactcgtc gccggaattc accagagttt
     2641 caaagttatc aggcatgtgc ttagtgatgg aaaatttgga tcagctatgc accccgagct
     2701 gtctggatgt cgaacggaga atcatgacct ttcccggagc gcccgggctt cgcccgcttc
     2761 tgcgacacgg gcactggagg cgtagcgaaa ctgcgaagaa ccggcggcct gcggcaggag
     2821 gagccagtat gggatcctcc ggcccgctcc aaagggactc attaataccc ccccttgact
     2881 ccgtcattat aatagcggtc ggggtgaggc gctcactcaa gcactggatt ggacgagttc
     2941 tcgggaagtc cgggccgcct gtggtagccg tattcctgag cggggacgcc gctctctcgc
     3001 ggtcgatggc gctgcggatg cgtgaactgg cgccggatta tcctcatgtc atcatcggcg
     3061 ccggcgagat tgatcggtcg catttcaagt acgcgcaaga catcatcgtc gtcaatcccc
     3121 accggcttct ggcaagctgg ctggagttgc ggcggcgcct gggacgccgc tggatcgctc
     3181 tggcgccgtt tctctggcaa cgcggagagc aaagcgaagc ggatagctac aggctgcttc
     3241 ggtggctgcc gtggctgctg gcgccgcgca agctgctggc tttcaacgcg cgcctggaac
     3301 gccatcacgt gcgcctgcgg ctgccgatag cctcgtggcg ttttctgcgc ggcgaaccgg
     3361 taggagatat tctgcggccc acgcctctcg gccacctgaa aaaacttcgg gctttggccg
     3421 gctttccgct ggtggcggcg tgctgggccg cggtgcagat gcggcggagg ccgccagccc
     3481 ctcttccgcc ggccagccaa gcccgtcccg gcgtaaccgc tttggctctg ccggaaaaaa
     3541 tcgacggcgc agtctgcgcg gaattggatc ggtcggtctg cggcgcggac accgagcata
     3601 tcctgatcat caccggcggg cgggaacttc cgccggcaac acgcctgcgg ctggaggcgc
     3661 tcctcgaaag accgcatacc tggctcgcct atactggccg aaaaatttcc gtagccgcgg
     3721 gagtgccggt cgatgcgccg gtgaagaccg gcgaggatgg gacctgggcc ctttgcagcg
     3781 atcccgctgc ggcgctgttt cgccgcgacg tttatctcgc tctgggagga atggcaagtt
     3841 tagagggggc ctatggcggg gctgcatggc tcgctctttc gctccttggc tggcagcgtg
     3901 gctggcatac gcttttcgca ggagcggccg aggccccgat aggccgcgac ttcgcccagg
     3961 aacgcctcat gctgggcgcc atttgtgatt tccgaacggc aatccggatc ctctgggaat
     4021 ggtctgggga ttggaggcgc tggaagggcg cgctgtcggc tctgggcggc atcgctacgc
     4081 cggctcccgc ctcggccgcc gcgctcgatc cgggagcaag agtctccgga tcctgccggg
     4141 atttttggcc gctgctcgac ccgcgagtcc atgtttttca ggggcgcagg ccgagcgggc
     4201 gccagcaggt ggctgtggtc agcccttact tgccgttccc gctttcgcac ggcggcgcca
     4261 tccgcatttt caatctgacc cgtactgcgg gcggccacgc cgacatttcc ctgttcgcgt
     4321 tcgccgagaa ggaaaccggg cgcgagatcg agccgctgtt gcacagttgc acgcgcgtgg
     4381 ttctggtgga gacgccgcgc tgggagcctg cgggattgtt tcgcctctgg ccgcgagggg
     4441 tatgcaaatt ccgctcgcgc atcatgcggg cggccatttc gcggctcgtc cgggacgagc
     4501 agatctcggt cgtgcagatc gagtacacac aactggccca tctgcgttcc gccgcggccg
     4561 ctcccgccgg taccgtgctg gtcgagcacg atgtgacctt tgatctttac cggcaactgc
     4621 ggatccgcgc gcaaggctgg gagaaggtga aagcccggct ggaggagctt cgctggaaac
     4681 gatttgagct gggttttgca cgcgccttcg accgcgtgat cgctatgtcg caagaagaaa
     4741 gggcacggct cctgcgcggc ggcctgccgc cggaacgcgt cgcggtggtg gagaatggcg
     4801 tagatctcga gcgcttccgg ccgttggcgc cggagcccgc cgcgctcccg gaactgctgt
     4861 tcattggatc gttccgccat tttcccaatg tgctcggatt cgagtttcta gttcgccagg
     4921 tctggccggt tctccgccag accaacccag agctcacgct caccgttgtg gccggcgcag
     4981 accatgagta ttactgggag cggcatacca acacgaaatg gcgggaaaca gccgggattg
     5041 acgttttggg cttcgtggaa gatgtgcgcc cgctctaccg gcgggctact gtcgtggcaa
     5101 tccctttact ggtctccgct ggcaccaata tcaaggttct ggaggccctg gctatggggc
     5161 ggcccgtggt ctccactctc gtcggcgtgg cgggcctcgg gctggtcacc ggggaaaacg
     5221 ttctgatcgc cgacaccgct caggagttcg ccgaagccgt atcacgcctg ctgggagacg
     5281 ccctgctgcg cgcggccatc gcccggcgag ggcgggagtt cgtcgaagag cgctatggat
     5341 gggacgccct cggcgagaaa ttggccggcc tttgggccga tcccaatctc aaggccccgg
     5401 aagcgtcagc catgtcctcc aaagcgtatc gaccagcgcg tgcatagttg cggaagccat
     5461 caggctcctg cgctgctggt aggccgatcc gtagaaccag cccgctatgg aagccagaac
     5521 gacataccgc cagttgggaa atgaagcatg gcggatatgg ctgagtccaa acagaaggga
     5581 agcagttgct tgcgccacca ggcgggaatg ccaggttctt tccagcaggt tctgtaagat
     5641 cccgcgaaag taaagctcct ccgcgacggc gatgaagaca aatatcgtcg ccagatcgaa
     5701 gctgaatgcc cagagcccgc gccatctcgg attccagccg atgaagcgca tggcaaagcc
     5761 cagcggtatt gcgattgctg cgaacagggc gaaatggacg gcggcatccc gaatgaccgc
     5821 cagcgaaaac gaaaattcgt agccggaacc gtccagccct cgccagagca ggaaggacca
     5881 cgcgcccacg gccaccagaa gcagccgggc catgaaatcg aggttcaccg gtacattcca
     5941 cactcctcga atctggcccg agaaaatggg aagcgccagc caaatcagca ccagcaggtc
     6001 ctgccagttc atccggtccg gaaatcgcac cggcaaaagc tcgtagagaa aaaatggtac
     6061 agccacgaag aggagcagct tcagaaaagc catccggcgg aagtcaccta ccccggaagc
     6121 gtaaaccaga tacggcgcaa gaaacagacc cacacagagc gccgccggcc gcgcgccatg
     6181 caggcgggct gccagccacc ggcgcgatac gcgaaatccc ggcgcgagca acagtgcaaa
     6241 aagcaggagc aggcaagcgt agatggaagc cgccggaact gcggcgaccg ggtagcgccg
     6301 cagctccatc ctggcgctga aggcgaaaaa tacggcgatt gcgccataag cggcggcagg
     6361 aggcgcccat tgccggcttg tgtcttccat agaaatccgc ttttcaggtt agcgccaaat
     6421 cccgctgcaa ggtatactgc gcccggagga gtcatgcggc tcgcatggtt gtttctgttt
     6481 tgggccggcc tgtcgttcgg ccaactgaag ggtattatcg atctgcatgc gcactgcgat
     6541 ccggacagca tgcctcgctc ggtggatgct ctggaattgg cgcggctggc gcaaaaagaa
     6601 ggcatgcggg cgctgctgct caagaaccac ttcgcgccca cggcgcagct tgcctatatc
     6661 gtcagccatg ctgtgagcgg catacaggct tatggcggca tcgtgctcaa cgggcctgtc
     6721 ggcggcatca acgccgcggc gattgagcaa atggctcgct tcaagggcgg ctacggcagg
     6781 gtagtgtgga tgccgacgtt tgacgcggaa aaccaggtcc gcgtatccaa agagaaccgg
     6841 cctttcgtcc cggtttcccg caacggcgtt ctcctgcctg aagtgattcg ggtaatcggc
     6901 atcattgcgc gggaaaagct ggcgctggcg accggccatt cttctccagc cgaggatttg
     6961 atgctgatcc gggaagcgcg ccgcgctggc ataacccgga tcatcgtcac ccacgccatg
     7021 ttcagtcccg ttagcatgtc cgtcgagcag cagaaggaag cggcgaagct aggagcctac
     7081 attgagtttt gctacaatgc gctgctgccg gcggcaggcg ccaaccgcct tcagcccgcc
     7141 gactacgtgc gcgctattca cgccgtggga gcggagcact gcgtgctgtc gagcgacctc
     7201 ggccaggcga acaaccctgt gcacacagaa ggctggaaga tgtatctcga tacgctgcgg
     7261 aaggcgggaa ttaccgagag cgagatcgac tggatggcgc ggcgtaatcc ggcgcggctg
     7321 ctggccctgg aataggactg gagcttctcg ggatcacggc cagagcttct tccgtgtagt
     7381 tattggaagg actccagagc aggtagccgg catacgcatc gcccagggca tcctgaaccg
     7441 ctttgatctg ggcctcaacc ctatctttgt cgtaccgggc cccgatattg aaatcctgca
     7501 accacggacg cagccggccg cgcacgggcc gggcgttccg cagcctcttt ccggctgatc
     7561 ccaaggaata attcactaca cggtaaggct gttcagcggg attcgggaaa ccaagaaagc
     7621 cggcggcaaa gtgcgacgga tataccatcg ggcaaacgta atcgaagaaa gggtaagcgt
     7681 cttcaatcac ctgcccaatg ccgaggtcgt cgtgattgac ggtggaaagg ccaaacagat
     7741 cggctgacag tacggcgtcc ggcatttcgc ggcgcacata agcgaagaac cggcggatca
     7801 cttcgcgttt gggcgtggcg ccatcccaga cgggaaattc catttgtttc aggtcgccgt
     7861 ctgaaggaaa gcggacgtaa tcaaaattga tctcatcgaa gccccggctc aaagcttctt
     7921 tcgcgagcgc ggcgttatag gaccaagctt cccgggaggc cggatcgatc cacgccagat
     7981 gcttccggtc gagccacaat accgccgccg gccgcagcgc cgccccagcg ccatcgcttt
     8041 ccgccggcgg cgcctcaaga tgctgatgca cggccagctc gggccgcgca agggctagcc
     8101 gcgggtcctg gaacacgaca atccgcgcta tgacgtaaag cccttcccga tgtagtcggc
     8161 cgagcaacga atcgacgtct cggatggtca cgcgccgggc cccgtagcga gcggcgtcgg
     8221 gcgcggttgt agcatacgcg acgtagccgg ttgcgtcctt aatgtcgatt actaccgtgt
     8281 tgatgcggcc cgcctgcgca agctcgatga cccgacggat gcgggccggc gatccggcgg
     8341 accagcttgt caggtaaacg ccttgaaacg cgccaggcga agtccggccg ggaggcttct
     8401 ggctccacgc gaagaggatg gccccgcaga tcaggaatgc ggttaatacg tgccgtctcg
     8461 agaaatgacc gttctggatc aaaacatttt ccctatctat tggatgatac cgggttgtga
     8521 gccgtttcac caatagtgcc ggtatccgcc gtcctgtgtt actgtgtcgg agctcgttct
     8581 gggagaagaa gatgagaccc attctggttc tgatcgcatt ggccggcata tgtctcgccg
     8641 cgccgaaaac cgagaccggc gaactgaacg gcgcccaatt tcgcatcgac attcccgacc
     8701 gctggaacgg cgggttgatc ctgtattgcc acggttacaa cgatacgccc gtacgttatg
     8761 acggcgccaa accgaacccg ctcatcagca ttttcaccgg ccagggctat gcggtcgccc
     8821 agtcgggcta tgcggccggc ggatgggcga tccaggaagc gctggaggac acggaggccc
     8881 tgcggcgtta cttcaccgcc aaatacgggc agcccaagga aacctacgta acggggcact
     8941 cgatgggcgg cttcctgacc atggaactca tcgagacatt cccaaactcc tacgacggcg
     9001 gcctggcctt atgtgggccg ctgggggcgg caagctggtt catgctgcgg cgggtcttcg
     9061 atatgcgggt cgtatttgat tactattttc ccggcgtgct gccttcaccg gtgaaagttc
     9121 cggcggattt ctcgcggacg cctcagcgtg tggccgaggt tctgaaagcg ctcgaagaga
     9181 agccggagcc agccgcggcg atgcgacgct actcaaacat tcggacgaac aaggaactgg
     9241 cgggcacaat cgtcttctgg acctacatac tgaaggatct ccagcaaaga ggcggcggca
     9301 atcccttcga caatcggaac accatttacg acggcacgcc tgatgacaac gcagtcaata
     9361 gcggcgtcgg tcgatataag gccgacgccc aggcagccga ttacatccgc cactattaca
     9421 cgcctacggg acgtctgacc cgtcctctgc tcgccattca ctcgacctac gatgtcctgg
     9481 tgccgccgtg ggtgaccaat cagtattcca tcatcgccgc agaggcgggc agctctgact
     9541 ggttcgtgca gcaatatgtc aagcatgatg ggcactgcgc gattttaccc gacgaaatcc
     9601 ggcgcggttt tgaccaactg cgcgagtgga aaaccatcgg caagcggccg ccggcgggag
     9661 aaaaccgtta agtcgcccgg ccaggttgga cccaggattt gcttcccgtg ctcccgtaat
     9721 acacccgcta gggatccagc catacgcccg gacgcgatca cggcgacagc accagtacgc
     9781 cgtaaggccg gtcgccgact ttgatcttct tgattactgc ctgcttggcc agatccacaa
     9841 cggagacgtc atttgacggc ccgtttgccg tatacagttt cttgccgtcg ccggaaagcg
     9901 cgatgcccca cgggcgctgg ccgacttcaa tggaaccagt gatctggtcc gtggccgtcg
     9961 agatgacaaa caccctgtgg ccgcccccgc tcgatacgta cacctgcgat ccgtccggcg
    10021 agactactac gcccatcgga cggaattgtt tgccgcccag cggaatcgtt ttgatcaatt
    10081 cctgacgaac tgcgtccact actgctacca ggttgtccac ttcacaagtt acataggcgc
    10141 gggagccgtc gggcaggaac gctacggagc gcgccctgtg gcccactttg aaattcttga
    10201 ccaccttgcc cgccctcgcg tcgatcacaa acaacgcgcc ctcgccttcc gaagtgacat
    10261 atacgaattt gccgtccggg gtcattgtta ccccctcggg ctccgatccc acgggaagcg
    10321 cttgcgccac cttctcggac gccaagtcaa aaatgctggc cgtggcggca tcctcgttgg
    10381 aaacatagag aagctttccg tcgcggctca aagcaaattg ttcaggatca ttgccgcttt
    10441 tcatcacctt cagcagcttg ttctgctgca catcgaaaac gccgatcccg tcagcgctgc
    10501 ggtcgggagg cggcagtttg ctttcatcca ctccaggtcc cgcaaagggg gagccgctga
    10561 gagcaatgta aatggtccgg cggtcggggc tagcgtgtat cccgcgcggc cgctttccca
    10621 ggggaacagt cgcgattacc tcgagtttct cggcgtcgat gacgctcagg tcgccggatc
    10681 cctcattggt aacgtaaata cgctcgccct gggactcctt tcgttcctga gtccgggaat
    10741 tgccgcagcc ggacagaaag ccggccaggc agagtaaaac aaacagagaa ggggctcggt
    10801 tcacggtccg agtatacccg aatttacgaa gggctttttc cacttcgcgc gtcgccagtg
    10861 gccggccggt gagcgcggcg gccggccgcc gtggaagtcc ctgctttgcc aggcccttga
    10921 caacatccgc ccggcgctgg atactcgggc agcgttgcca ggaggctcgc catgctgaac
    10981 cgccgtgaat ttctgactac gaccgcttta ggaatcggcg ccgctgccgt tgcccaggcc
    11041 ctgccgtcag acaaaatgcg ctttgccatg agcggccacg aatttcggaa gatgcagccc
    11101 cacccggaag caggcatcaa ggtgacagcc cgctacggct atcacgggct ggagcccttt
    11161 caggaagacg tagccaagta cctcgacaag ccccccgaag cgctcaagca ggtgctcgag
    11221 gcctcaggtc tggccctttg cacgatcggc agcggcggcc agtatcagga tccggccaag
    11281 attccccaga cgatcgaaaa caacgtcgcg cgctgccgtt atatcgcttc cttcggctgc
    11341 aagcatctca aagtgaactt gaaccggcgt cctggagacg gccgcgccaa tctcagcccg
    11401 gaagacgcta aggcgctcgc ccgcacgctg aatgagatcg gtaagagaac gatgggagaa
    11461 ggcggggtga aattcgcgtt tcatccgcac tgctggacca tcgtcgagcg cgagccggaa
    11521 ctgcggcaga taatggacct gaccgatccc aaatttgtct acttgattgc ggacacctgt
    11581 catttgagcc tgggcggcat cgatccggca aaagctgttc gggattacta ttcgcgcttg
    11641 gcggccgtac acctcaagga ttgtgagccg aaatacagcc ccgctcgtgg atggaaaggc
    11701 cctgcgccca gcgaggagga acacaaccgc gtcaatctct acaagcggct gggaaccggc
    11761 ggcgtggatt ttcccgccct cttccactta ctgcgagaaa gagactacga cggctgggtg
    11821 acattggatt tcgacgctcc acggcccggc gaaggcactg tcgaggaaga tatgaactcc
    11881 cacagaaaat atctgatgga gacgcttcac gtcacgctgc gcaatgggca gggttagaca
    11941 cgccgatgga cacggcctct ggcgcgactg gtctttggcg aggaccggcg ttgcgcacgc
    12001 aagtggaggt gatacagtag cactgaggaa aaagatggct cgagttacga ggattgcttc
    12061 cgggaaaaag gtccctgccg ccgcagtgcg ggccggcggg aagagaatgc cgagcttcgc
    12121 gccgttcttc tgcggcgtct gcgcgtttgt gctgctattg gcatttgcag ggggcgccgg
    12181 cagggcggct ccctccgggc ctgcctcgga aacatggatt ttcgatcgca tcgatcggat
    12241 cggcggccat cccactaccg ttctcggcca cccgcgcgtg attgactctc ccgtaggcaa
    12301 ggccgtcgag ttcaacggag tagacgatgc tctgttcatc ggcgttcatc cgctggccgg
    12361 ggccgaaacg ttcacctggg aggccatctt ccgtcccgat ggcggcgcga gagagcagcg
    12421 gtggttccat ttgcaggaat cgggatcgga taatcggatg ctttttgaaa tccgagtcac
    12481 gcccgaaggc caatggtttc tcgacagctt caaccagacc ggagcggctt cgaaggcgct
    12541 gatcaaccgc aacgcgctgc acccgaccgg cgcttggtat catgtggccg cggtctacga
    12601 tggacgggag tttcgcaact atgtgaacgg gattcaggag ggggctgccg agattcatct
    12661 ggccccacag gggcccgggc aaacctccgt cggcgtgcgt atcaacaagg tgaattactt
    12721 caagggagcg gttcacctgg cgcgtttcac tcgccgcgcg ctcacgccgg ccgagttcct
    12781 caagctgcct ccaggggccg cgtccggaac gggaaacggg tcgcgctaat ttctccggcg
    12841 ctgctattta gcgctttcct gctccagagg cttgcccgtc agcagccggg cgagcaccgg
    12901 tagcggcaca gccccctccc gcagtgcctg ctcattgaaa tccttgaggc ggtagccgcc
    12961 atttttgacc ctgttgtact gctcgcggac gcgcagccaa tcccgccagc caacgcggta
    13021 cgtgggcaac tggcaggagg aaagttttgc tcgctggagc ttggctgccg cctcctcatg
    13081 ctcctggaag gtgtctttct ccatcaagtc catggcctgc tggtcggtca ttcccatggt
    13141 ttgcaggcgg atgtccagta tggcgttggc gagaacccgc agttcctctt tgaggaacgt
    13201 aagccgcatc tccggagaac tgtcgaagcc ttcgtcgagc atgagctgct cggcgtactg
    13261 gccccagcct tcgatgtaag gcgtattgcc aaacacagcg cgcaaaatgc gccgggcttt
    13321 gggctggatc tggttcgcat actcgagctg cacatagtgg ccgggcatcg cctcgtgaat
    13381 cgtaagcagt ttcagcttgt agtagttata ctcccgcagc ttcgattcca cgcgctgggc
    13441 gggccattct ttcggtatgg gcgtgatcca gtaaaaggcc ccaagttgcg gttccagagc
    13501 aggcgcagcg ttgaagccgc ccaccccgta aatgccgcgc atgaactcgg gagtctcgat
    13561 caccagcaaa ttctcgtggg gcggcagggt aagcaggtct ttcgcgcgga caaactcccg
    13621 cgcttcctcc agatctttgc gggcgtcggg aatgtagctc tcgcgggtcg agtgccgccg
    13681 ggcaatgtgg tccagggtct cgccgatcac gcgggtctcg tctttgacat cgcgcttgcc
    13741 cggaaacatc ttttggtaca agggcaaggc cagctcgtac atccgggcgc gcacggactt
    13801 cacgtccgct tcggccgacg ccagcacctg ctccggggtg agatcggttt cgagcacgta
    13861 ccggaatttc ttctgatatc gttcttttcc cagccgccag tcggcgttcg tgcgtttcgc
    13921 gagatcgttc ctcaagtagt cctgaaacgc ccggagcgcc gcgacggccg gtttggacgc
    13981 actgtcatac tcggcgcgca gctcttcggg caaactggcc cggatgatgt tttcaatcag
    14041 gttcagattg ccctcgtttt cctccagcgc cacccgagtc cagatttccg gagcggcggc
    14101 caggttcgct ttcgcctggt cgagaaacga ggggatcttt ttcatccgtt cgatgatctg
    14161 gcggacgcgt acgtccttgg gcgcgtattc gagaacgtgc gggttgaaaa gcgcattgcc
    14221 gatcaattcg acatataagg tcgggttgtg gcggtaattc tgaatttcat ccagctcgag
    14281 gagattcagg gcgatctggt cctggataat gtcaaaatct gcgcgatctt ccggctccaa
    14341 ctgtgcggga tcgacggcat cgctcagccg gcggcgaaag ccggtgtaaa actgccgctg
    14401 ctggtcgatg cccgccgggc tgagatcgtc gagcatctcg tcgagcttcc gttcggcgcc
    14461 cgttttcggg tctttatgaa cgtggtatcc cgtggctgta gcgctcaccg gagaaagcgc
    14521 gagagtcgaa tagacgaact cctccgccaa tgaagcaaat gcctcgggca ttttgcgcct
    14581 gccgcatccg gccgcagcca gagcgcccaa caggaacacg atccagcaac gcctttgcag
    14641 catggcttgt gattttaccg caaagtcact cggccaagga ttcctggatg acgggaagct
    14701 cggtcttcac tccgaattgg ccttcccacc gcgccacgac ggccgaggcc agacaattgc
    14761 cgatcacgtt gaccgaggtc cgccccatgt ccatgagagc atcgacgccc agtaggacca
    14821 gaaccggctc catcggcaga tggaaggaag aagccgtcgc cagcaggatc accagcgccg
    14881 cccgcggtac tcccgcgacg cccttgctgg tcaacatcag agtcagcatc atgaacactt
    14941 gctggccgaa gccgagatgc attcccgccg actgggccac gaacatcgac gccaaagaca
    15001 agtagagcgt tgtgccatcc agattgaagc tgtagccggt gggtattacg aaagccacaa
    15061 tgggacgcgg aaccccgatt gcttccatcg actccatggc gcggggcaag gcggcctccg
    15121 aactggtggt ggcgaaggcg atcgacgcgg gctcggcgac cgcgcgggcg aatgcccgaa
    15181 tggggactcc ggcaatcttc gccgccggca gcaaaacgag caccagaaaa gcggccagcg
    15241 ccgcgtataa cgtccctagc agcttggcga ggctggccag aatgccgaga cccatgtgcc
    15301 caaccgtgta tgctatggcg gatccaacgc cgaccggcgc aaaaagcatc acgatgttgg
    15361 tgaactggaa catggtgtcg gaaaggcttt cgcaaaaggc cagcatcggc cggcgctttc
    15421 gctcgcttag catcgccagg gctacgccaa agacgatact gaagacgacg atttgcagga
    15481 tctggccctc cgccaccgac tgggcgatat tttccggaaa aacgtgaaga atcacgtcgc
    15541 tggcaggcgg ccgggccgcc tgcacgggcg ccttttcagc ggggagcgcc gttgtagaat
    15601 ggcgagaagc ttgaatgccg attgcgctgg gaggaagccg gactcctacc ccggcgcggc
    15661 tgatgttaat agccgccagg ccgataaaca gcgcgatcgt ggtaaccatc tcgaaatata
    15721 caagcgcctt tatccccatg cggccgatct gtttcagatc cgcgtgcccg gcgatgccga
    15781 ccacgagcgt ggcaaacagc agcggcgcaa tcaccgcccg gatcatgcgg agaaaaatca
    15841 gacttaaaac ctggagcttg accgcggttc cgggccagtc gtagccgaac tcggcgccgg
    15901 cggccatgct gaccagaatc caagccgtca gcgaacgccg cctggcggcc cagacagcca
    15961 ggatgccaat caggagccag cgcgcgatct ggaaaattgt ttcctgcaca agccgccagt
    16021 gtcatgcata gtcatgttga agtcaaacgg cgcaggaaaa gcgcgagtag tgggatcccg
    16081 agagggcgtg gtaagatacg cgggaacact gtcccggccc aagccggcga agcaagtccg
    16141 ggcggatctt atgtcagtgg aatgccaaga ttccttggcg ggttcgcagc cactccgaac
    16201 agagatcatg aagcttggag tcctcgatgt gattccgtcc ggcgcgcggc tgagggcggc
    16261 tcaaaccggc aaaaacccgc gccgcgaatt ggaggaggtg tgatgaaaat acgttttcaa
    16321 ccgattttgg ggctcgcagt aatcgcgtgg catctggcga cggccttggc cgcctctgcg
    16381 cagacggggg cgaaggacgg cgagtggcgc acttacggag gcgacctggg gaacacgcgg
    16441 tactcgccgc tcgatcaaat caacgcctcc aacttcaaag acctggaagt cgcatggcga
    16501 ttcaagaccg attccctggg gccccggccg gaatttcaat tcgaagcaac gccgctgatg
    16561 gttcacggca aactttactc taccgccgga tcgcgccgcg cggtgatcgc cctcgatgcg
    16621 gccaccggcg aactgctctg gatgcacagc gagaatgaag gcgcccgcgg cgaagcggct
    16681 ccgcgccagc tctcgggccg aggcctggcg tactggaccg acggcaagga agaacgcatt
    16741 ttgtacgtta cccccggcta ccgcctgatt gccctcaatg ccaaaaccgg cgtccccatt
    16801 gccagcttcg gcaaaaacgg cgtggtcgat ttgaagcagg acgacgatca ggacatcgat
    16861 ttggtgaccg gagaagtcgg gctccatgcg gcgccgatca tcgccaagaa cgtcgtgatt
    16921 gtcggtgcgg cgcacatgtc cggcagcgtg ccgaagagca aaaagaatgt gaaaggctat
    16981 gtccgcggat tcgacgtgcg cactggcaag cgcctgtgga tttttcatac gatcccgcgg
    17041 cccggcgagt ttggaatcga aacctgggag aaggattcct ggtcttacac gggaaatgcc
    17101 ggtgtgtggg ggcagatatc tgtggacgaa gacttagggc tggcctactt gcccgtggaa
    17161 ctgccgact
//
LOCUS       DASREK010000200        11282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_591733, whole genome shotgun sequence.
ACCESSION   DASREK010000200 DASREK010000000
VERSION     DASREK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11282
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591733"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFA54_10380"
     CDS             <1..998
                     /locus_tag="VFA54_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZS51258.1"
                     /translation="NLRLSGFFRLVSAWVVEHAHRPLALLASVVLVAGVFSAFFVNDT
                     MCLVLTPLVLEITTVLRRNAVPYLLAVAMASNIGSAATITGNPQNMMIGSFSRIGYRR
                     FAAALSPVALIGLLATVGIIALVYRKEIFAQGRVEIAQPAVRVNRVLMWKALAVSAGM
                     IVFFFWGWPVPKVALIAGAVLLITRRIKPEKVYREIDWSLLVLFVGLFIVVAGVEKTP
                     FSQELLAVASHFRLEEAAVMSAAAAVLSNIVSNVPAVLLFKPYLARLPDPARAWLTLA
                     MASTLAGNLTVLGSVANLIVIQRARHEVKISFGEYFKVGAPLTAITILAGVLLLS"
     gene            1305..2390
                     /locus_tag="VFA54_10385"
     CDS             1305..2390
                     /locus_tag="VFA54_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZS51259.1"
                     /translation="MILSLVLFSAAASAAVSTQPTHKSTAGRRRARPRVAGQTWTESS
                     LADSTAGDLVDGEDLTVRQAAVDALGPLNGSVVVVDPNSGRVLTMVNQKLALAGGFTP
                     CSTIKVPVALAALNEGIISLHTKLRLGKQWYMNLTDALAVSNNVFFARLGEALGFQRV
                     QQYARMFGFGEKAGWNIPGEQLGTFPSAPPKNGGVGRLTSFGEEIAATPLQQAAFVSA
                     LANGGTLYYLQYPRTEEEISNFIPKVKRRLDIARLIPYVLPGMTAAVKRGTARRAYDP
                     SDPIMGKTGTCSENGARLGWFTSYNDLGNKLAVVVLLRGGRDVFGPRAAEIAGHVYRN
                     LTERHYFAAQRPLTPAAFIAGDSCCSR"
     gene            2476..4458
                     /gene="mutL"
                     /locus_tag="VFA54_10390"
     CDS             2476..4458
                     /gene="mutL"
                     /locus_tag="VFA54_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZS51260.1"
                     /translation="MGVIRILPDQVANKIAAGEVVERPASVIKELLENALDAGASRLR
                     IEVEAGGRRLMRVADDGRGMLRDDALLAFERHATSKLRDIGDLLSISTLGFRGEALPS
                     IASVARVQLDTRAASERTGTRVEIAGGKLMAVEEAALETGTTVTVRDLFFNVPARRKF
                     LRSEQTELAHVASLVTHYSLAHPEKSFSLATPKGDLLSVSPVQSVRDRVFQIFGSEAL
                     DELVALGESQKELVISPAWPDPAEAVAAARAERESGGAALEKKIFRLSGFVSRPQVQK
                     LNRNSIYIFVNRRLIRDRLLLHALSSAYHNLMPAGCYPFALLFLEVPFDQVDVNVHPS
                     KTEVRFRHQSFVHDFLRDTIRARLMESKPVAEFPTAPRELVAKSGTAPSPAASMPYSE
                     ASLLLMEADAGLPPSDFQLQPGPAPAARFDFGAAPAIEIGPEPGGPPAAGLSPAEPPA
                     PRQNSSLVPSEKLESLASLGSLRPLGQVRDSFIIAAGPDGLWIIDQHVAHERILFEKV
                     LRERSQGTAERQQLLMPMIVRLSAAQQIGFLRLAEELHAAGFEIEPFGRGTIAVKAAP
                     AGLSAHEIEGLLGEVLDTPESELRNLSPDDLRRKIAASIACHAAIKINTPLDQRKMHW
                     LLDELAKTGCPMSCPHGRPIVLRYSMKEILKAFHRT"
     gene            4562..8053
                     /gene="dnaE"
                     /locus_tag="VFA54_10395"
     CDS             4562..8053
                     /gene="dnaE"
                     /locus_tag="VFA54_10395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZS51261.1"
                     /translation="MTAAPFVHLHCHTDYSLLDGACEIKQLMELVEKQNMPAVAMTDH
                     GNLFGAVQFYDEAHKRGIKPIIGCEAYVTQQSRSVTAESNRYNHLVLLCENLEGYRNL
                     IQLVSSGYLDGFYYKPRIDKDLLAAHSKGLIALSACLRGEVNETLLEKEFEEGRRLAY
                     TYLDIFGKDNFFLEVQDHGLEQDRKLLPNVYRLSQQTGIPLVATNDSHYLRREDARAH
                     EVLLCIQTGKTMSDPARMKWSTPDFYLKSRDEMLRLFGDCPEALDHTWDIAQRCNLKL
                     EKVKEPFPRFDVPEGHTADTYFEFVAREGFERRRPRLEMLRAQGRLKHDLPEYMERLD
                     SEIRMIQKMKFSGYFLIVWDFIRFAKSRGIPVGPGRGSAAGSLVSYAMQITDLDPLEH
                     GLLFERFLNPERISMPDIDIDFCMNRRGEVIQYVTEKYGRDRVAQIITFSTLGTKAAI
                     KDVGRALDLPYGDVDRLTKLVPNVLNIKLKTALEQEARLREAAQKDPRIGEVMEIAQR
                     LEGVARNASVHAAGVVISPQPLQELVPLYKTNRDEIVTQYDMNGLEKLSLLKMDFLGL
                     ATLTVVDDALKLIEAGRGEKIVLEELPLEDPETYAIFSRGFTSGVFQFESEGMRDILR
                     RYQPTRLEDLTALNALYRPGPIQGGMIPDFIDRKHGRRPVTYDLPELEEILAETYGVI
                     LYQEQVMQISNRIAGYSLGEADILRRAMGKKKPEEMAAQRERFIAGAVARGFPRKKVE
                     KLFDLMEQFAGYGFNKSHSAAYAYLAFVTAYLKAHYPVEFMSALLSSETGNTAKIVKY
                     INECREMGIRVLPPDVNSSDWNFTPAGEAIRFGLGAVRNLGQNAVAAIHAARNEVGRF
                     RSLFHFCEHVDLKCLNKRTIESMIKAGAMDGLGGTRAQLFAAVDRAIEAGQKANRDRE
                     SGQHALFGMPSAADAPQSERLPEVEPWSEREQLKGEKEVLGFYVTGHPLNAYEEKIRE
                     LATADSSTIEALAAQGSGNADVALCGIISNVSVKRNREGKPWAAVQLEDRSGNVDLLI
                     FAAAFESLRPYLEPDQAVLVRGTLRLEENSPPKVAVSDITPLDNARIRLPGRLSIRIR
                     LVGPAGEAAAGSEIAEKLAELFRRKPGDTDVCFRLERPGDFLVLLDSGLRVRADREFC
                     EAASSILGPGSVEIVPV"
     gene            8050..9000
                     /locus_tag="VFA54_10400"
     CDS             8050..9000
                     /locus_tag="VFA54_10400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZS51262.1"
                     /translation="MNIDPEMIRQRIAHLESEIAELRAIQSDPAVEALLGELAAEVDA
                     LKQQSSDGPPAWGRVQLARHPQRPHTLDYISRLFTNFQEIHGDRRFGDDPAIVCGMAR
                     FGGREVMIVGQQKGRDTKQNLQRNFGMPKPEGYRKALRAMQLAARYGRPILTFLDTPG
                     AYPGVDAEERGQAEAIANNLREMARLPAPIVVTCLGEGGSGGALALGVGNSVLMLENA
                     VYSVIAPESCAAIIWRDSGKAALAAEALKLTAEDLLRLGLVDEIVPEPPGGAHLDHEA
                     AAKLLREALERTVTHLCSLSGTELVSQRYEKFRRMGNFFA"
     gene            complement(9010..9690)
                     /locus_tag="VFA54_10405"
     CDS             complement(9010..9690)
                     /locus_tag="VFA54_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZS51263.1"
                     /translation="MSSTATNLTTFAVGSEDPLTLLPHTPVRAYKKNEVIYGPEDRVE
                     ALYLVVDGRVKISRLTEGGKEVILDFYHKDDFFGETGFLGSDHRGERASALDATSVML
                     WTIPELKRLMQRTPALGPALIRVVTQKLADANQRIESFCLDAINRRLVKALLHLAERF
                     GQQGEAPYVHLLPITHEQLARYVGTSREIVTQYMNQFRHDGLLRYSRRGVDLDIGALK
                     KYLGEVQA"
     gene            complement(10263..10526)
                     /locus_tag="VFA54_10410"
     CDS             complement(10263..10526)
                     /locus_tag="VFA54_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZS51264.1"
                     /translation="MGTSRQFTCKHLLRELTDYLDGSLDEEMRTQLEAHLAKCPNCWV
                     VCDTTKKTIEVFKGMEPYPLPQDVKSRLMKALEKRCGQTGQAH"
     gene            complement(10498..11097)
                     /locus_tag="VFA54_10415"
     CDS             complement(10498..11097)
                     /locus_tag="VFA54_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS51265.1"
                     /translation="MATEQELVEQAKRGDTSAFGELVNRYERNIYRLARHITQNEDDA
                     EDVLQETFLKAFANLDQFQGHSKFYTWLVRIAVNESLMKLRRRKADRSVSLDEPVETG
                     EDSMPREIAAWEENPEQKYGRAEMNAILSRAIDSLSPGFRTVFLLRDVEGFSTEETAS
                     ALKLSIPAVKSRLLRARLQLREKLNRIFRKHGNITPVHL"
BASE COUNT         2199 a         3370 c         3462 g         2251 t
ORIGIN      
        1 ccaatctgcg gctttcgggg tttttccgcc ttgtgagcgc ctgggtggtg gaacacgcac
       61 accggcccct ggcgctgctg gcttcggtgg ttctggtggc gggcgtattc tccgcgtttt
      121 tcgtgaatga cacgatgtgc cttgtgctga cgccgctggt gcttgaaatc acgaccgtgc
      181 tgcggcgcaa cgccgtcccc tacctgctgg cagtcgcgat ggcttcgaat atcggcagcg
      241 cggccacgat tacgggcaac ccgcagaaca tgatgatcgg cagcttttcc cgcatcggct
      301 atcggcgctt cgccgctgcc ctgtcgccgg tcgccttgat cgggctgctg gccaccgtcg
      361 gcatcatcgc gctggtttac cggaaggaaa ttttcgcgca agggcgagtc gagatcgccc
      421 agccggccgt tcgcgtcaac cgcgtattga tgtggaaagc gctggccgtg tcggccggca
      481 tgatcgtctt ttttttctgg ggctggcctg ttcccaaggt ggcgctcatc gccggtgcgg
      541 tcctgctgat cacccggcgc atcaagccgg aaaaagtcta ccgcgaaatt gattggtcgc
      601 tgctggtgct gtttgtcggt ttgtttattg tggtcgcggg agtggaaaag acgccgtttt
      661 cccaggagct gctcgctgtg gcgtcgcact tccgcctgga agaagctgcg gtgatgagcg
      721 ctgcggcggc ggtgctgtcg aacatcgtga gcaatgtgcc ggcggtgctg cttttcaagc
      781 cttacctggc ccggctgccc gacccggcgc gggcctggct gacactggcc atggcctcca
      841 ctctggcagg aaatctgacg gtgctcggat cagtggccaa cctgatcgtg attcaacggg
      901 cgcggcacga agtgaagatc agctttggag aatacttcaa agtgggggcg ccgcttacgg
      961 cgatcaccat cttggccggt gtgctgctgc tctcctagcc cgattctttt gctttctttt
     1021 acgagttttg ccgaaaaatg cggcgccccc gccgagcccg tccgcgcacc ggcaggccgc
     1081 ggagcgaagg ccgcagcccg gttgcagacc ggaaaagacg gggctgggag cgcctgcacg
     1141 ccggaaaagt cttgacttca aatacttgta ctctgaaaat agaagcgaag acagggcgtt
     1201 tagttgctgg gatgtcgcct tgcctcgcgc tgtagctgca aaatacggcg gtttggtgtg
     1261 aggagtccgt gccacgaact caaacgttgt ttttttgttt ctcattgata ctttctcttg
     1321 ttcttttcag tgcggcggct tccgccgctg tttcgacgca acccacgcac aaatccactg
     1381 ccggccggag acgggcgcgg ccgagagtcg ccggccagac ctggactgag tcttcccttg
     1441 ccgattcaac cgccggcgat ttggtggatg gagaggatct gaccgtgcgg caggccgctg
     1501 tggatgcgct gggaccgctg aacggcagcg tggtcgttgt ggaccctaac agcggccgcg
     1561 tgctcacgat ggttaaccaa aagctggcgc tggccggagg atttacgccg tgctccacga
     1621 tcaaggttcc cgtggcgctg gctgcattga acgaaggcat catcagtttg cacacgaaac
     1681 tgcggttggg gaagcaatgg tacatgaact tgactgacgc gctggccgtt tccaacaacg
     1741 ttttcttcgc gaggctcggc gaggcgctcg ggtttcagcg cgtgcagcag tatgcccgta
     1801 tgttcgggtt cggcgagaag gccgggtgga acatcccggg agaacagctc gggacatttc
     1861 ccagcgcgcc gcccaagaac ggcggcgtcg gccgcttgac cagtttcggc gaagagatcg
     1921 ccgccacgcc tttgcagcaa gcggcgttcg tttcggcgct ggccaacggc ggcacgcttt
     1981 actacttgca atatccgcgg accgaagaag agatctcgaa ttttattccc aaggtgaagc
     2041 gccgcctgga tatcgcgcgc ctgatccctt acgttcttcc gggcatgacg gcggccgtga
     2101 aacggggcac agcgcggcgc gcctatgatc cctctgaccc cattatgggg aagacgggga
     2161 cttgcagcga gaacggcgcg cgcctcggct ggtttacttc ttataacgat ctgggcaaca
     2221 agctggcggt ggtggtgctg ctgcgcggcg ggcgcgacgt gtttggcccg cgcgcggcgg
     2281 agatcgccgg ccacgtttac agaaacctta ccgagcgcca ctatttcgca gcccagcggc
     2341 cgttgacgcc ggccgccttt atcgccggcg atagctgctg ctcgcgatag ccaaagtcaa
     2401 caatctggag attgttacaa gcttcagcgc agcaaacccc agcttgctca aagctgcggg
     2461 ctgagggctg tccgcatggg cgttatccgc attctgcccg accaggtcgc caacaagatc
     2521 gctgccggcg aggtggtgga gagacccgcg tcagtcatca aggagctgct ggaaaacgct
     2581 ctggacgccg gcgccagccg tttgcggatc gaagtcgagg ccggcggccg gcggctcatg
     2641 cgagtcgccg acgacggccg cggcatgctt cgcgatgacg ccctgctggc gtttgagcgc
     2701 cacgccacca gcaaattgcg cgatatcggc gacctgcttt ccatttctac cctgggattc
     2761 cgcggcgagg cgctgccgtc cattgcctcg gtagcccgcg tgcagctcga cacgcgggcg
     2821 gcgagcgagc ggacgggcac gcgcgtcgag atcgccggcg gaaagttgat ggccgtggag
     2881 gaggcggcgc tcgagaccgg caccacggtc acggtccggg atctgttctt caatgtgccg
     2941 gcgcggcgca aatttttgcg ttcggagcag acggaattgg ctcacgtcgc ttcgctggtt
     3001 acccactaca gcctggccca tccggagaaa agcttttctc tcgcgacgcc gaagggagat
     3061 ctgcttagtg tcagcccggt gcagagcgtg cgggatcgcg tgtttcaaat attcggctcg
     3121 gaagcgctgg acgaattggt ggcgcttggg gagagccaaa aagagcttgt catctctcct
     3181 gcctggcccg acccggcgga ggccgtggcg gccgcccgcg cggaacgcga atccggcggc
     3241 gccgccttgg agaaaaagat attccggctc agcggttttg tatcccggcc tcaggttcag
     3301 aaactgaatc gcaactcgat ctacattttc gtgaaccggc ggctcatccg cgatcgcctg
     3361 ctgttgcacg ccctttccag cgcctaccac aacctgatgc cggccggctg ctatcctttc
     3421 gcgctgcttt ttcttgaggt cccttttgat caagtggatg tgaacgttca tccgtcgaag
     3481 accgaagtac ggttccggca tcaatccttc gttcacgatt tcctccgcga cacgattcgc
     3541 gcccgcctga tggagagcaa gcctgttgcg gaatttccaa ctgcgccgcg ggagttggtc
     3601 gcgaagagcg gcacggcgcc cagcccggcg gcttcgatgc cgtattcgga agcttccctg
     3661 ctgctgatgg aggccgacgc cggcttgccg ccttccgatt tccagttgca gccgggcccg
     3721 gcgccggcgg cgcgattcga tttcggcgcc gcgcctgcca ttgaaatcgg gccggagccg
     3781 ggcgggcctc cggcagccgg ccttagtccg gctgagcccc ccgcgccgcg ccagaattcc
     3841 tctctggtcc cctcggagaa gctcgaatcc ctggcgtcgc ttggcagctt gcggccgctc
     3901 ggccaggtgc gggacagttt tatcattgcc gccgggccgg atggtctgtg gatcatcgat
     3961 caacacgtgg cgcacgagcg cattctcttc gaaaaggttt tgcgcgagcg gtcccaggga
     4021 accgccgaac ggcagcagtt gctgatgccg atgatcgtga gattgtcggc cgctcagcag
     4081 atcggctttc tccggctggc cgaggagctg cacgcggcag gatttgaaat cgagccgttc
     4141 ggccggggga cgattgccgt caaagcggcc ccggcggggc tttccgctca cgaaattgaa
     4201 ggcctgctgg gcgaggttct cgacacgccg gaaagcgagc ttcggaatct ctcgcccgac
     4261 gatctccgcc gcaagattgc agcctccatc gcctgccatg ccgccatcaa gatcaacacg
     4321 ccgctcgacc agcgcaagat gcactggctg ctggacgagc tcgccaagac cggctgcccg
     4381 atgagctgcc ctcacggccg gccgatcgtc cttcgataca gcatgaagga aatcctcaag
     4441 gcgtttcaca gaacctgacc gccgcgcccg ccgggcgccg ccgctctcgt caggaaaggg
     4501 ccgtgtgagc ggcgctccgc aggctcgtga atcgccagtt tgggtatact gaaatttccc
     4561 catgacagcc gcccccttcg tacatctgca ctgccacaca gactactccc tgctggacgg
     4621 agcctgcgaa atcaagcagc tcatggaact ggtcgagaag caaaacatgc cggcggtggc
     4681 gatgacggac cacgggaatc ttttcggcgc tgttcagttc tacgatgaag ctcataagcg
     4741 cggcatcaag cccatcatcg gctgcgaggc ttatgtaacc cagcagagcc ggagcgtgac
     4801 ggccgaatcc aaccgctata accatcttgt gctgctgtgt gaaaacctgg aagggtaccg
     4861 caacctgatt caactggtgt cgagcggtta tctcgacggg ttttattaca agccgagaat
     4921 cgataaagac ctgttggcgg cccacagcaa gggcttaatc gctctttccg cctgcctgcg
     4981 cggcgaggtc aacgagacgc tgctggaaaa ggaatttgag gaagggcggc gcctggccta
     5041 tacgtacctc gacattttcg gcaaagataa ttttttcctg gaagttcagg atcatgggct
     5101 ggagcaagac cgcaaactcc tgccgaacgt ctaccggctt tcgcagcaga ccggcatccc
     5161 gctggtcgcc acgaacgact ctcactacct gcgccgggag gacgcgcgcg cccacgaggt
     5221 gctgttgtgc atccagacgg gcaaaacaat gagcgacccg gcgcgcatga agtggtctac
     5281 tcccgatttc tatctgaagt cccgcgacga gatgctgcga ctcttcggcg actgtccgga
     5341 agcgctcgat cacacctggg atattgcgca gcgctgcaat ctcaagctcg agaaagtgaa
     5401 ggaacccttc ccccggtttg acgttccgga gggccacacc gccgatacct acttcgagtt
     5461 cgtggccaga gagggattcg agaggcgccg cccgcggctc gaaatgctcc gcgcccaggg
     5521 acgcctcaaa cacgatctgc cggaatacat ggagcgtctg gacagcgaaa tccggatgat
     5581 ccagaagatg aaattttccg gctattttct gattgtctgg gacttcatcc gcttcgccaa
     5641 atcgcgcggc attccggtcg gccctggccg gggatcggcg gccggcagcc tggtcagcta
     5701 tgcgatgcag atcaccgatc tcgatccgct cgagcacggc ctgcttttcg agcgcttcct
     5761 gaacccggag cgcatctcga tgccggatat cgatatcgac ttttgcatga atcggcgggg
     5821 cgaggtcatc cagtatgtca ccgagaaata cgggcgcgac cgcgtggctc agatcatcac
     5881 cttttccacg ctcggcacga aagcggcgat caaagacgtc ggccgcgccc ttgatcttcc
     5941 gtacggcgat gtggaccggc tcacgaagct tgtccccaat gtcctaaaca tcaaattaaa
     6001 gaccgccctc gagcaggagg cgaggctgcg cgaggcggcc cagaaggacc cccgcatcgg
     6061 cgaggtgatg gagattgcac agcggctgga aggagttgcg cgcaacgctt cggtgcatgc
     6121 cgcgggcgta gtgatttccc cgcagccgct ccaggagctt gtcccgcttt acaagacaaa
     6181 ccgcgacgaa attgtcacgc aatatgacat gaacgggctg gagaagctgt cgcttctcaa
     6241 gatggacttc ctcgggctcg ccaccctgac cgtggtggat gacgcgctga agctgatcga
     6301 agcgggacgc ggcgagaaaa tcgtcctcga ggaattgccg ctggaggatc cggaaaccta
     6361 cgcgattttt tcgcgcggct tcaccagcgg cgtgtttcag tttgaaagcg aagggatgcg
     6421 cgacattcta cgccgctatc agccgacccg gctcgaggac ttgacggctc tcaacgcgct
     6481 gtatcgtccg ggcccgattc agggcggcat gatccccgat ttcatcgacc ggaagcatgg
     6541 ccggcgcccg gtgacctacg atctgccgga attggaggag attctcgccg aaacatatgg
     6601 cgtgatcctg tatcaggaac aggtgatgca gatctccaac cgcatcgccg ggtattccct
     6661 cggcgaggct gacattctgc ggcgcgccat gggcaaaaag aagcctgaag agatggccgc
     6721 ccagcgagaa cgcttcatcg caggggctgt ggcgcgcggc ttcccgcgca aaaaggtcga
     6781 aaaacttttc gacttaatgg agcagttcgc cggctatgga ttcaataaat cgcattcggc
     6841 cgcctacgcc tatctggcct ttgtcaccgc gtacctgaag gcccactatc ccgtggaatt
     6901 catgtcggcc ctgctcagct cggaaacagg gaatacggcc aagatcgtga agtacatcaa
     6961 cgaatgtcgg gagatgggaa tcagagttct ccccccggac gtcaactcga gcgattggaa
     7021 tttcacgccc gcgggcgaag cgatccggtt cggcctggga gcggtcagga atctcggaca
     7081 aaacgccgtg gcggcgattc atgccgcgcg caacgaggtg ggccgctttc gatcgctgtt
     7141 tcacttctgc gagcacgtgg acctgaaatg tttgaacaag aggacgattg agagcatgat
     7201 caaggccggc gctatggacg ggcttggcgg aacccgcgcc cagcttttcg cggccgtcga
     7261 tcgagccatc gaggccggcc agaaagcgaa ccgggaccgc gaaagcgggc agcacgcgct
     7321 gttcggtatg ccttccgcgg ccgacgcccc gcaaagcgag aggcttccag aggtcgaacc
     7381 ttggagcgag cgggagcagc tcaaggggga aaaggaggtc ctcggctttt acgtcacagg
     7441 gcatcccttg aacgcctacg aggaaaaaat ccgggaactc gccactgccg acagctcgac
     7501 gattgaagcc ctcgcggcgc agggcagcgg aaacgccgac gtcgcgctgt gcggcatcat
     7561 ttccaatgtc agcgtgaagc gaaatcgcga aggaaagccc tgggcggcgg ttcagttgga
     7621 ggaccgctcg ggcaacgtgg accttttgat cttcgccgca gctttcgagt cgctgcggcc
     7681 gtatctcgaa cccgatcaag ctgtgctggt tcgcggaacc ctgcgcctgg aagagaacag
     7741 cccgccgaag gtggccgtct cggacatcac cccgctcgac aacgcccgga tccgcttgcc
     7801 gggcaggctt tcgataagga tccgcctggt gggtccggcc ggagaagcgg cggccggctc
     7861 ggagatagcg gaaaaattgg cggagttgtt tcgaaggaag ccgggagaca ccgatgtttg
     7921 tttccggctg gagcgccccg gcgatttttt ggtgctgctc gattccggct tgcgagtgcg
     7981 ggccgaccgt gaattctgcg aagcggcgag ctcgatcctc ggtccaggct cggtggagat
     8041 agtgcccgta tgaacatcga tccggagatg atccgtcagc gaatcgctca tctggaaagc
     8101 gagatcgccg agttgcgcgc aatccagtcg gatccagcgg tcgaggccct gctcggcgag
     8161 ctggccgcgg aggtcgatgc gcttaaacag cagagcagcg acggcccgcc cgcgtggggc
     8221 cgtgtgcaac tggcgcggca tccgcagcgc ccgcacacgc tcgattacat ttcccggctg
     8281 ttcaccaact tccaggaaat tcacggcgat cgccgcttcg gcgacgatcc ggcgattgtc
     8341 tgtggcatgg cgaggttcgg cgggcgggag gtcatgatcg tcggtcagca gaaagggcgc
     8401 gacacaaagc agaatctcca gcggaatttc ggcatgccga aaccggaagg ataccggaag
     8461 gccctgcgag ccatgcagct cgccgcccgg tatggccggc ctattctgac tttcctcgat
     8521 actcccggag cctatcccgg cgtggacgcc gaagagcggg ggcaggcgga agcgatcgcc
     8581 aataatcttc gggaaatggc ccggctgccg gcgcccattg tggttacctg cctcggtgaa
     8641 gggggcagcg gcggagcgct cgcgctcggc gtcggcaact cggtgttgat gctcgagaac
     8701 gccgtctaca gcgtaattgc gccggaaagc tgcgccgcca tcatctggcg cgactcggga
     8761 aaagctgctc tggcggcaga agccttgaaa ctgaccgccg aggatttgct tcgcctgggg
     8821 ctagtggatg aaattgtacc cgagcctccg ggcggcgcgc atctcgacca cgaagcagcg
     8881 gccaaactcc tgcgcgaggc gcttgagcgt acggtcactc acctttgctc cctgagcgga
     8941 acggaattgg tgtcccagcg gtatgagaaa ttccgccgga tgggaaactt cttcgcgtaa
     9001 aatctggcct caggcttgta cttcacctaa atatttcttc agggccccaa tgtcgagatc
     9061 gacgccccgg cgcgagtacc gcagcaggcc gtcgtgccgg aactggttca tgtattgggt
     9121 gacgatttcc cgcgatgtgc cgacatagcg cgccaattgc tcgtgagtaa tgggcagaag
     9181 atggacgtag ggagcctctc cctgctggcc gaaccgctct gccaagtgca atagagcctt
     9241 caccagccgg cggttgatcg catctaagca gaagctctcg atccgctgat tggcatccgc
     9301 caatttctgt gtcactacgc gtataagtgc gggtcccagt gccggcgtgc gctgcatcag
     9361 gcgtttcagc tcgggaatgg tccagagcat taccgaagta gcatccagag cggaagctcg
     9421 ttctccgcgg tggtccgatc ccaggaatcc agtctcaccg aagaaatcat ctttgtggta
     9481 aaagtccaga atcacttcct tgccgccctc ggtaaggcgg ctgatcttta cgcggccgtc
     9541 caccaccaga taaagggcct ccactcgatc ctcaggacca tagatgacct cgtttttctt
     9601 atacgcgcgg acaggagtat gcggcagtaa ggtgagcgga tcctcagaac cgactgcaaa
     9661 tgtcgtcaga tttgttgctg tggaagacat tcaacacccc ttgaccatgc gtgcaggtga
     9721 cgggtgcaga ggagaccaag gagggcgtat caggtgtggt gaacgagacg ttgaaacaag
     9781 cggctcccag ccggcaaggc tcccctcacc ctcatccccc tctcagcccc gacggattcg
     9841 accgtcgcaa aaggctgctt ggcaactcgc atctcggccc agtcatatgc aaaatgcttg
     9901 ccacaatcca agcacacaac gtaagtttcc tcacttcgct tcccccggcg gcctgtggag
     9961 ggatcccgaa gggtaattgg gaagcttgtc cgccggtgag aacaaccaaa cagccagttg
    10021 gcgacggtcg aaagcatatc tccgaggtac tgcacctccc tgtctgttag attgcattcg
    10081 atctctccag gttgcagaaa tccggttgcg ctggactgta acaccgcgcg agggaactac
    10141 catgcagtcg cgaccagcag gactgcggcc aaacaagctg ggaattcggg gaagggaatg
    10201 caaggaatag ggaggaaatg cctgggagaa gcgcaatcgg ggcggcgggg aagccgataa
    10261 attcagtgcg cctggccggt ctgcccgcac cgtttttcga gcgccttcat caaacggctc
    10321 ttgacgtcct gcggcagcgg atagggctcc atccctttga atacctcgat ggtttttttc
    10381 gtcgtgtcac aaacgaccca gcagttggga cacttggcga gatgagcctc gagctgcgtg
    10441 cgcatttcct catccaatga gccgtcgaga taatcggtca gctcccggag caggtgctta
    10501 caagtgaact ggcgtgatgt tcccatgttt gcgaaaaatc cgattcagtt tttcccgcag
    10561 ttggagccgc gcccggagca accgcgactt gacggcggga atggaaagct tcaacgcgga
    10621 agctgtctct tcggttgaga atccctctac atcgcgcagg agaaagacgg tgcgaaaccc
    10681 cggcgagagg gaatcaatgg cgcgcgacaa aatcgcattc atttccgccc ggccgtactt
    10741 ctgctccgga ttttcttccc aggcggctat ctcgcggggc atgctgtctt ctcccgtttc
    10801 cacgggctcg tcgagcgata ccgacctgtc ggctttccgc cggcgcaact tcatcaacga
    10861 ttcatttacc gctatgcgga ccagccaagt ataaaacttg gagtggccct ggaactggtc
    10921 cagatttgca aaggccttaa ggaacgtctc ctgtagaaca tcttccgcgt cgtcttcatt
    10981 ctgggtgatg tgccgggcca gacgataaat attccgctca taccggttca caagctcgcc
    11041 aaaagcggac gtatcgcctc gcttagcttg ctcgaccaac tcttgttcag tcgccacatc
    11101 cggcgagcta cctgattgga tcgccacgat ttaaaaaagt tccagatatc tcggcccatc
    11161 ggagttgctg cgcgggaggc cgattctggc tcctatccca atggtacact acaacttgcc
    11221 gctccgcagc tcggactttc cgtctctttt gtgcttttat ggaacgccaa aacatcaaat
    11281 ta
//
LOCUS       DASREK010000201         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1085715, whole genome shotgun sequence.
ACCESSION   DASREK010000201 DASREK010000000
VERSION     DASREK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085715"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFA54_10420"
     CDS             <1..426
                     /locus_tag="VFA54_10420"
                     /inference="COORDINATES: protein motif:HMM:NF020680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HZS51266.1"
                     /translation="KLAAWAQGRPGCALRIDVATYERRREAMLSLLRTASGGPFGELI
                     RYSEGIGRSKSERLSLHLDSLYTLLADLLHIQQGTGRLVNEDIRPELASLARKVDFQW
                     IAKAARQIDELDALERRNIQKQIALEALSVSLRRPGASL"
     gene            628..1032
                     /locus_tag="VFA54_10425"
     CDS             628..1032
                     /locus_tag="VFA54_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51267.1"
                     /translation="METRNSNSFMSYTRNDSIGNKRTNNMESANRKLIRPSLTEFKEQ
                     QAAQGSQRKPQKRPVPPEQTNAENFYYVKQMQARTPMVVVLNDGEELHGVIEWYDRGC
                     IKVNRNGGPNLLIYKANIKYLYKENDKNGEAS"
     gene            complement(1099..1614)
                     /locus_tag="VFA54_10430"
     CDS             complement(1099..1614)
                     /locus_tag="VFA54_10430"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HZS51268.1"
                     /translation="MNEKLLIPKPVRESQSELSELALPNDANGLGNLLGGKLMHLMDI
                     AGSLAAARHCRSYVVTASFDHMNFLAPIQIGQLVLLYSSVNRAFRTSMEVGVKVYVED
                     LRTGERKHVSSAYLTFVALDEQRRPVPVPPVIPETEQEKRRYEEAGRRRAYRLQLAGK
                     SRLQNGGLAPR"
     gene            complement(1611..2036)
                     /locus_tag="VFA54_10435"
     CDS             complement(1611..2036)
                     /locus_tag="VFA54_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51269.1"
                     /translation="MACPYFCPTERLPRALWPHPDRLPLGGGFDGFCRARPAAEFRPG
                     DARLREHCNLGYARGECGDFPPDADSDAVRFGISEEREEIVLVRYVLERDHRPFAHGF
                     LRYHAGSRRLLSAPADPVLQRQAEAYADGYLRSKLRSSS"
BASE COUNT          481 a          635 c          659 g          417 t
ORIGIN      
        1 aagctggccg cgtgggcgca gggccggccc ggttgcgcgt tgcgcatcga cgtggcaact
       61 tacgaacggc gccgcgaagc catgctgtct ttgctgcgca cggcaagcgg cgggcctttc
      121 ggcgagctca tccgttattc cgaaggaatc gggcgcagca agagcgagcg gttgagcttg
      181 catctcgatt cactctatac gctgctcgcg gatctgttgc atatccaaca aggaacgggc
      241 cgcctggtca atgaggacat ccgccccgag ctggcgtccc tggcgcggaa agtcgatttc
      301 cagtggattg ccaaagccgc ccggcagatt gacgagctgg acgccctgga gcgccgcaac
      361 atacagaagc aaatcgctct ggaagcgctc tcggtgagcc tccggcggcc tggcgcgtcg
      421 ctatgaaaaa gttcgttgtt gcacgcaggc attctttgtg atatcaataa aaacagactg
      481 gaagccaacc agtgcgtagc tgaccggatc acggaatacg agcccccttt tcggcatagc
      541 aagcaattcc gctgcctgaa ggtctgctcc tcttccctgc cggcgccgag agtggttgcc
      601 tttttttaaa ttaagaggag ggtcagtttg gaaacccgga attcgaactc cttcatgtct
      661 tatacacgca atgattccat cggtaataag agaacaaata atatggagtc ggccaatcgt
      721 aaattgatcc gcccgtcttt gacggaattc aaagagcagc aggctgctca aggttctcaa
      781 agaaagcccc agaaacggcc tgtcccccct gagcagacca atgcggagaa tttctattac
      841 gtcaaacaga tgcaggcccg cacgcctatg gtcgtggtgc tgaatgacgg cgaagagttg
      901 cacggcgtta ttgaatggta cgatcgcggc tgcattaaag taaaccgcaa cggcggcccc
      961 aacctgctga tctataaagc caacatcaag tatttgtaca aagaaaacga caagaacggc
     1021 gaagcaagct gatcgggagc gcgcgggctg gcgcgaagcc tcgccgccgg ggcgggctgg
     1081 ttcggaccaa gccggagatt accggggagc caatccgccg ttctgaaggc gcgatttgcc
     1141 ggccagttgc agccggtaag cacgccgccg ccccgcctcc tcgtaccgcc gcttttcctg
     1201 ttcggtctcc ggaatcacgg ggggcacggg aacgggcctt ctttgttcat ccaaggcgac
     1261 gaaagtcaga taggccgagc tcacatgttt tcgctcgccc gttcgcaggt cctcgacata
     1321 caccttcacg ccaacttcca tggaagtccg aaaagctctg tttaccgaag agtataaaag
     1381 tacaagctgc ccgatctgaa tgggcgccag aaagttcata tggtcaaacg aggcggtcac
     1441 gacgtaagag cggcagtggc gggcggcggc gagcgagccg gcgatgtcca tcaggtgcat
     1501 cagcttcccg cccagtaggt tacccaatcc attggcgtcg ttgggcagag ccagctcgga
     1561 gagctccgat tgcgactcgc ggaccggctt ggggatgagc agcttttcat tcatgaggac
     1621 gaacgcagtt tcgagcgcag atagccgtcg gcataagcct cagcctggcg ctggagcact
     1681 ggatcggcgg gagcagaaag gagccggcgg ctgcccgcat ggtaccgaag aaagccatgc
     1741 gcgaagggcc tgtgatcccg ttccagcaca tagcggacca atacgatttc ttcgcgctct
     1801 tctgatatcc caaaccgtac tgcgtcggaa tcggcgtccg gcggaaagtc gccgcattcg
     1861 ccgcgggcat aaccgagatt acagtgctcg cggagcctgg catcgcctgg gcgaaactcc
     1921 gccgccgggc gcgctcggca aaacccgtcg aagccgccgc cgaggggcag ccggtccggg
     1981 tggggccaga gcgcgcgggg cagccgttcc gtcgggcaga agtacgggca tgccatcgcg
     2041 gtgtgtcgct tgctatagta gtggcttccc cgtgccggtg caacatgcaa aaatcacgcc
     2101 tcgaaacgct ccgcgacttt gttgccgcca atcccaatga ttcgtttgtg cgctacggcc
     2161 tggcgcagga atatctgaaa cttgatcaag cg
//
LOCUS       DASREK010000202         4673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1916518, whole genome shotgun sequence.
ACCESSION   DASREK010000202 DASREK010000000
VERSION     DASREK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916518"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFA54_10440"
     CDS             <1..302
                     /locus_tag="VFA54_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51270.1"
                     /translation="IRVQWFASNAEKGPAISDVTLHVFLEKENAIGQAAVPKPSAQAP
                     YESALVMDFEPGARSTGDFSLQAPEAGNYLLRVETIGASKKLGREYFAAMDVKIQ"
     gene            337..918
                     /locus_tag="VFA54_10445"
     CDS             337..918
                     /locus_tag="VFA54_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51271.1"
                     /translation="MQPRPLKDYRAAGDGIGSRTSLLKRPRLAGRSILLPAVLAALLG
                     IAAPPFARAEHFDIKLLAAGPDGVTQEAYADQSPPECGLNPRPVLKARAGQKITMQFI
                     MTNVYPHGIAKNAGIHYYVVREKEAGQKTLPPLNQGVVTEGTFTFNLKPKARIGAREH
                     LVIREPGVYLLRVESQRTQRDHEHFSAIDLLIQ"
     gene            981..2141
                     /locus_tag="VFA54_10450"
     CDS             981..2141
                     /locus_tag="VFA54_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZS51272.1"
                     /translation="MLGLSLAAADTTAKSAQADKSWLAIPFLEIFNARDADPAISESS
                     VAIPAGGRSISGFLVRPIVAGRLPALLLLSGGGEIPDWLRQSAREIASTGFVALAPAY
                     DPEGLGSNSTLVQAVAGDQLGTNVDAALSWLASQAFVDAQRVGAAGWGEEGAEQVLRL
                     AQRGKIQGGVVADTAACNNPDRWLQVRAVPLLIIQGAGSCTAAKAAALQQMFARAQLP
                     HALHLYPGVSGSFLQSTAAPGPAAAADRAWVDIYEFLAKNVEDTNPAAVPASSQPQNE
                     IARIVDIMRVINADDGVRGELARSLAALPAGNAQWERARSQAAMLAEAGNLLLERRPP
                     KGSLTGWRERAAEFRSAAQAVLRAVEQRDFPAAQEALRRLPQACAACHADYR"
     gene            complement(2173..3585)
                     /locus_tag="VFA54_10455"
     CDS             complement(2173..3585)
                     /locus_tag="VFA54_10455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZS51273.1"
                     /translation="MTSIPNQGSQGKPAGRGASGAAQLNDALLNPHGRNAPDALNGSL
                     LEQVKPGSPESDDLSGPPATNEQLEKQFSLITETQQKLAEVKMQAARAMMSKSTDTIQ
                     EKTEEGQSLVSLLNLRLAALEKDLTAARRARPKDPAVQWLTGELLLIVGGEPEEIEPY
                     LNRAATAGLKRPQLYVSLARVQFESNRFQEAYENALKAVEADKQSRTAWEIYMRTAFG
                     IERFGEVVQRLDQSFPNVKPPWAGAIRRSAMDLLNQWRHEQALRHAEDRANNLPMVRL
                     MIEHRVFAGTPDGKNPAQVKTLGRGQVIIELFEDQAPASVANFLNLVEQGFYDGTRFH
                     WSDGGRMVVGGDPNTKNNDPEDDGPGGPGYAIPDESTRPGARGFFRGTLGVVQNGPRT
                     AGSQFFIALVPIPEFNGHFTAFGRVVKGQEVIDQITIGRTNREIGQSGRIIPGDVLVR
                     AEVVRKRAHPYEVTRLAPAR"
     gene            3974..>4673
                     /locus_tag="VFA54_10460"
     CDS             3974..>4673
                     /locus_tag="VFA54_10460"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HZS51274.1"
                     /translation="MRILREFSTSFAASAALFAPFLFASLALAAGKGPQEYLVYVGTY
                     TGPKSKGIYAYRFQPSTGKISPIGLVAETRNPSFLALHPNGRYLYAVNEVNDYQGQRA
                     GSVSSFAIDPKTGKLTLLNQVSSRGAGPCHLVVDKSGKNVLVANYNSGSVAVLPVRAD
                     GSLGEAAAFVQHQGSSVNPRRQQGPHAHCVALSPDNRFALVADLGLDEVLIYRFDAAK
                     GLLTPNSPPFAKVSP"
BASE COUNT          893 a         1454 c         1412 g          914 t
ORIGIN      
        1 cgattcgggt gcaatggttt gcatcaaatg cggaaaaagg cccggctata tccgacgtga
       61 ccttgcacgt ttttctggaa aaggagaatg ccatcgggca ggcggctgtc ccaaaaccca
      121 gcgcacaagc gccttatgaa agcgctttgg tcatggactt tgagccggga gcaaggagca
      181 ccggcgattt ctcgctccag gcccccgaag ccggcaacta cctgctgcgg gtcgaaacga
      241 tcggtgcgag caagaagctg ggccgcgaat atttcgccgc catggacgta aaaattcaat
      301 aaacggccgc aaaagaacga acgtgaacgc ttgaccatgc aaccacgccc tctgaaagat
      361 tacagagccg caggcgacgg catcggatcc aggaccagcc tgctcaagcg gccccggctt
      421 gcgggccgct ctatcctcct gccggccgtt ctagcggcgc tgctgggaat agcggcgccg
      481 ccgtttgccc gcgccgagca cttcgatatc aaattgttgg ccgcggggcc ggacggcgta
      541 acgcaggaag cttatgccga ccaatcacct cccgaatgcg gtctgaatcc ccggcccgtg
      601 ctgaaggctc gcgccggcca gaagattacg atgcagttca tcatgacgaa cgtctatccc
      661 cacggaatcg caaagaacgc cggcatccat tactacgttg tgcgggagaa ggaagccggc
      721 caaaaaacgc tgcccccgct gaaccagggg gtggtcaccg agggaacgtt tacgttcaat
      781 ctgaaaccga aggcgcgcat tggcgcgcgg gagcatctcg tgatccggga gccgggcgtc
      841 tacctgctgc gggtggagtc ccagcgcacc cagagggacc acgaacattt ctcggctatc
      901 gacctgctga tccaatgaag cggtgaccct tgccccggca cagtctttta tcccgagtca
      961 tagcgcccgc agcagcggta ttgttggggc taagcctggc ggcggccgat acaaccgcga
     1021 agagcgctca ggctgacaaa tcgtggctgg ccattccttt cctggaaata ttcaatgcac
     1081 gcgacgccga ccctgccatc tccgagagtt cagtcgctat cccggcaggc ggccgcagca
     1141 tttcgggctt tttggtaagg ccaattgtgg ccggccgttt gcccgcttta ctgctgctct
     1201 ccggcggtgg agaaatcccg gactggctac gccaatcagc gcgcgagatt gccagcaccg
     1261 gtttcgtcgc tttggcccca gcctacgacc cggaaggctt gggatcgaac tcgacgctgg
     1321 tgcaggccgt agcgggcgac caactcggga ccaatgttga tgcggcgcta agctggcttg
     1381 ccagccaggc ttttgtcgac gcgcagcgcg tcggagcggc tggatgggga gaagaaggcg
     1441 cggagcaagt gctgcggctc gcacagagag gaaaaattca gggtggcgtg gtagcggata
     1501 ctgcggcttg caacaacccg gatcgctggc tgcaagtgcg cgccgttccc ctgctgatca
     1561 tccagggggc cggctcctgc accgctgcga aggcggccgc tctacagcag atgttcgccc
     1621 gggcgcagct tccccacgct cttcacttgt atcccggggt gagcggctcg tttctgcaat
     1681 ccaccgctgc cccggggccg gcggccgccg ctgatcgcgc gtgggtcgat atttatgaat
     1741 ttctagccaa gaatgtggag gacaccaatc ccgcggccgt tcccgcctcc agccagccgc
     1801 agaacgaaat cgcgcggatc gtggacatta tgcgcgtgat caacgccgat gacggagtgc
     1861 ggggagaatt ggcgcgctcc ctggcggctc tgccggccgg caatgcccag tgggaacgcg
     1921 cgcggtctca agccgcaatg ctcgcagagg ctggaaactt gcttttggag cggcgcccgc
     1981 ccaaaggatc gctcaccgga tggcgggaaa gagccgcgga atttcgcagc gctgctcagg
     2041 ccgtgctgcg cgcggtcgag caacgcgatt tccctgcggc gcaagaggcg ctgcgccgct
     2101 tgccgcaagc ctgcgccgcc tgccacgcgg actatcggtg aacctgccgg cttgatctga
     2161 tctcaacggc gcttaccgcg ccggagcgag tctggtgact tcgtacggat gggccctttt
     2221 gcggacgact tcggcgcgca ccagcacatc gccaggaata atccttcctg actggccaat
     2281 ttctcggttg gtccgcccga tcgtaatctg atctattact tcctgtccct tgactacgcg
     2341 gccaaacgcc gtgaaatggc cattaaattc cgggatggga accagagcaa tgaagaactg
     2401 actgcccgcc gtgcgcggcc cgttctgcac aacgcccaga gtcccccgaa aaaatccacg
     2461 cgcgccgggc cgtgtggatt catccggaat ggcgtagccc ggtccgcccg ggccgtcgtc
     2521 ttcaggatca ttgtttttcg tgtttggatc gccgccgacc accatgcgtc cgccgtcgga
     2581 ccaatggaaa cgcgtcccgt cgtaaaaacc ttgttcgacc agattgagaa aattcgccac
     2641 cgatgccggc gcctgatcct cgaacagctc gatgataacc tggccccgcc ccaaggtttt
     2701 tacttgggcg ggattctttc cgtccggcgt gccggcaaac acccggtgct cgatcataag
     2761 acggaccatc ggcaagttgt tggcgcggtc ttcggcatgc cgcagggcct gctcatgacg
     2821 ccattggttg agcaagtcca ttgcgctccg ccggatggcc cccgcccaag gaggcttcac
     2881 atttgggaag gactgatcca ggcgctgcac gacctcaccg aaccgctcga ttccgaaggc
     2941 ggtgcgcata taaatttccc aggcggtccg gctttgtttg tcggcttcga ccgccttgag
     3001 ggcgttttca taggcctcct gaaaacggtt gctttcgaac tgcacccggg ccaggctgac
     3061 gtagagctgc ggccgcttca gtcctgcggt tgctgcgcga ttcaaataag gctcgatctc
     3121 ctccggctcg ccgccgacaa tcagaaggag ctcccccgtg agccactgca ctgccgggtc
     3181 cttgggccgc gcccgccttg ccgcggtgag atctttctcc agagcggcga ggcgaaggtt
     3241 gagaagggaa acaagactct gcccttcttc cgttttctcc tggatcgtat ccgtgctctt
     3301 ggacatcatg gcgcgggcgg cctgcatttt cacttcggcg agcttctgct gcgtttccgt
     3361 aatcaacgaa aattgcttct ccaattgttc attcgtggcc ggcggaccgg aaagatcgtc
     3421 cgattccggg ctgcctggtt tgacctgctc gagcagactg ccgttgagag catccggcgc
     3481 attccggcca tggggattga gaagggcatc gttcagttgc gcagcgccgg acgcgccccg
     3541 accggccggc ttaccttgag aaccttggtt gggaatcgat gtcaccaacc gcgagggagc
     3601 cgcaggctgg gacctcctga cgaaccatac acccagcagg cacaagagca gcgcggcaac
     3661 cgttccggcc gcccaccgcg ctgcggatga gcgctgcgca ctcgttgtct ccgcagcggc
     3721 ggacgatgat cgcctttccg cgggaaatgc gccgccggct ggcgaactgg gctgttccgg
     3781 ctcgttcaaa tttctctcca ggcttctaca ctaccactat agacgggacc aggagggaac
     3841 gcagaacggg cgggcccaac gtttcggaac gccgctcctt agcgcaaaac aacggggccg
     3901 cgccccgccg atcgaggtcc ttttttggct ttttcgattg cgttttggca cgccgatgcg
     3961 ctttgataat cgcatgagga ttctgcggga gttttcgacg tcatttgcgg catcggcggc
     4021 gctgtttgcc ccgtttctgt tcgcttccct agcgctggcg gctgggaaag gcccgcaaga
     4081 gtatctggtc tacgtgggga cctacaccgg cccgaaaagc aaagggatct atgcctatcg
     4141 ttttcagccc tcgacaggca agatcagccc gatcggcctc gttgcggaaa cacgaaatcc
     4201 ttccttcctg gctttgcacc ctaacgggcg ctatctatac gcagtcaatg aagtcaacga
     4261 ttaccagggc cagcgagccg gatcggtaag ctcgtttgca attgatccga aaacaggtaa
     4321 gttgaccctg ttaaaccagg tttcctcgcg aggcgccggc ccgtgccatc ttgtggtgga
     4381 caagagcggg aaaaacgtgc tggttgcaaa ctacaacagc ggcagcgtgg ctgtactgcc
     4441 cgtccgggcg gatggaagcc ttggcgaagc ggcggctttc gtccagcacc agggatccag
     4501 tgtgaatccc cggcgccagc aaggcccgca cgcacattgc gtcgcgcttt cccctgataa
     4561 tcgctttgcg ctggtcgccg atctcggact ggatgaagtg ctcatttatc gcttcgacgc
     4621 cgcgaaggga ttgcttacgc cgaacagccc gccgttcgcc aaagtgagcc ccg
//
LOCUS       DASREK010000203        17498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2241306, whole genome shotgun sequence.
ACCESSION   DASREK010000203 DASREK010000000
VERSION     DASREK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17498
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241306"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VFA54_10465"
     CDS             <1..1148
                     /locus_tag="VFA54_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HZS51275.1"
                     /translation="SDTGVIPAVEVHWPEKGQTEWLRRLLTTLARAQFHGPSPARSEI
                     QRRLARSPVEVAEQLSRRYGHDLNDVAYQPALALIGRIWLGELTKDPQQLADVENIVA
                     PYFSGAKKTIPKKASSVNFAGHLVFGELARATGKSRYVNLAREAADYGFDEHGAPKEA
                     LPGHNEMSDAVFMGGPIEAQVGRLTGEAKYYDMSLRNMRFLLALDLRSDGLYRHSPLN
                     QAAWGRGNGFPALGLALSLSYFPPEQAGREEILRAFRAHMAALARFQDPTGAWHEVID
                     VPGSYRELTATCMIGFSMLRGVRSGWLEKDRYLPLAEKAWHAVRTRVAPDGGLVDVCI
                     STGKQPSLRAYLDRTAILGPDQRGGAMALLIATEVARWQAENPGWRP"
     gene            1269..1901
                     /locus_tag="VFA54_10470"
     CDS             1269..1901
                     /locus_tag="VFA54_10470"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HZS51276.1"
                     /translation="MLYCYFIARSGPELQKVSYRTVHPGPICFVQQHDLLTAVRALPP
                     GSRSTLPLMLEHNLVLSAASEQAAILPMPFGTAVSAEETLAQLLEARRAEILEALDRL
                     GGKAEMALRITLRPDEDPQDRAGQIAGICQPLQQWFEVRPDGSGEAVLEIAHLIHRQE
                     ADQYRKRLETQAAKVNGPRPPFHFLPPFLRKPVKSESRPGRAVCRRDATG"
     gene            complement(1906..2160)
                     /locus_tag="VFA54_10475"
     CDS             complement(1906..2160)
                     /locus_tag="VFA54_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51277.1"
                     /translation="MATPAATPEEIADEARKVRRLQLMMDLVMSVISQNDAMSIEEAS
                     ELIASARRAALNLFPGKELAYDLIYRPRFQRLMAEKYRIQ"
     gene            2273..2551
                     /locus_tag="VFA54_10480"
     CDS             2273..2551
                     /locus_tag="VFA54_10480"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZS51278.1"
                     /translation="MNPEMDQDNETLVNVFEAAGVSAEMEAQAVRALLDANGIEAVVV
                     RENVPELPVGKVEIRVVASDAERAREVIREGQLAGPAAAAEAEAESEL"
     gene            2769..3716
                     /locus_tag="VFA54_10485"
     CDS             2769..3716
                     /locus_tag="VFA54_10485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZS51279.1"
                     /translation="MRAEQETRPWALVWVGAGASLWGTDTFFRRPLAMSLSSAQIVLC
                     EHLILSVVLLPVFWQARSQWRALRLSQWGAVLGIAWGGSALGTICFTEAIKLGNPTAA
                     VFLQKTQPLFAAFLAALLLGERLQGRFWICLLFTVAGAYLVSFGESPAFFANPAVLAP
                     GRTAAALLALGAAALWGSSTVFGRFALQSVPFLTLTALRILCATPLLAAFAWLGRRQI
                     EVHLQTRQWLYLALMALVPGLLGLLVYYRGLRYTRASLAALAELSFPATAALLNWIFL
                     RTRITAIQAGGFLLVWLVIVYLDRSARRDKGNSAAVAAA"
     gene            complement(4080..5630)
                     /locus_tag="VFA54_10490"
     CDS             complement(4080..5630)
                     /locus_tag="VFA54_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZS51280.1"
                     /translation="MALYLVCVTLLLALWPSLLGAQARPQRDWLTFGGNPERTGWATQ
                     EVELNKGNASRLELKWKSQLDNVPTEMASLTAPLVVENVITPRGFKDLVFVAGSSDNL
                     YALDSDTGKVLWQKRFTNELKPKLPPRWLCPESLNATPVIDKQSRTIYLITSDGKLRG
                     LNIVNGEDRFPPTDFVPPFSKNWSLNLVNGVIYTTISQGCGEARSGVAAMDVSNPSRP
                     VTHFYTSIGGPAGIWGRAGAAFGPKGVYVQTGDGSWDPATGRWADTVLALEPKTLNLV
                     DYFTPRNREFVSKKDLDMGCASPVIFPFQKWQLVASAGKEGVIYLLDALALGGADHRT
                     PLFSARYSNDDIDFAGRGVWGAISTWQDSEGQRWLYVPTWGPPSKDAPKFQYTYGEAP
                     RGSIMAFQVTVQNDKPALAPVWISRDMSMPEPPVIANGVVFALANGENARQVGEDGVM
                     LTSKQRIDTRTGNAILYAFDAATGKQLYSSENIIDSWTHFSGLALASGRIYVTTFDSR
                     VYSFGLKQ"
     gene            complement(5689..6279)
                     /locus_tag="VFA54_10495"
     CDS             complement(5689..6279)
                     /locus_tag="VFA54_10495"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZS51281.1"
                     /translation="MPTTRLVVGICGATGAIYGIRLLEVLRNAAQIETHVVISKAAER
                     TIAWETSYTAEQVGRLAAFRHAVGDVGAALSSGSFQTAGMIIAPCSVKTLAAIAQGYA
                     DNLITRAADVTLKERRRLALLVREAPLTLAHIRNMAACAEMGAVIVPPVPAFYNHPKT
                     IDEIVNHTVGRILDLFSIETDLVRRWEGMAERQPAR"
     gene            complement(6283..6912)
                     /locus_tag="VFA54_10500"
     CDS             complement(6283..6912)
                     /locus_tag="VFA54_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZS51282.1"
                     /translation="MPFSCTCGTELPDNARFCFHCGKPQREQDLGASLAHSEGLPQGP
                     AREAPPAPPVNFGNPIAVRVALLCASLSALLNVIPFISFGCCLWILGAGFVSTVLYTR
                     RTGFLLSVADGARMGWLTGLLTFVIGLFLTAFSFLLARSAGGIREIIRQSVERMPAQD
                     SVAQQVRDLLASPVGIMIFLLSYIIFGFVMIVSLAVAGGALGAKVMEKE"
     gene            complement(6960..7535)
                     /locus_tag="VFA54_10505"
     CDS             complement(6960..7535)
                     /locus_tag="VFA54_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51283.1"
                     /translation="MNGSSAPLPPPSSAEPSTGRLVATVAAVLAGIVVLTLAGLGMAS
                     YLFVSRIHVARTPGGRGENTAIRIETPLGSVRVDKQERVDPKLLGIPLYPGAVVVEGE
                     AGGARVDVDLDFAGKSLRVAAVQMETPDPMEKVVSFYRSEASDFALRFESRGRAEFHS
                     SRDGIKKVIGIAAQRGKTRISLANIGAPEAN"
     gene            complement(7532..7993)
                     /locus_tag="VFA54_10510"
     CDS             complement(7532..7993)
                     /locus_tag="VFA54_10510"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HZS51284.1"
                     /translation="MWRGCSGNSIRWVAVTSPALVLLAAVSAPLLARAASPLSLGVYT
                     AFSWACHQQPLRSWSLDGRPLAICVRCFGFYLGALVGGLIGHRFSRRLFLASTSLMGG
                     EWLVETAIWPAVPGVIRFLTGLAVGFSLISILWTERVRPVLRMLKLGETAS"
     gene            complement(7999..9168)
                     /locus_tag="VFA54_10515"
     CDS             complement(7999..9168)
                     /locus_tag="VFA54_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZS51285.1"
                     /translation="MFPFRGVDYLEIDSLFSEQELLVRRTARQFVEERVIPIIEKHNR
                     EGSFPLHLVPEMAELGFLGANLTGYGCAGMSNVEYGLIMQELERGDSSLRSFCSVQGA
                     LVMYPILTYGSEEQKQRWLPRLQNGTAIGCFGLTEPDFGSNPAGMRTTAVRDGPGYIL
                     NGEKTWITNGSLADVAVIWARLDGVIRGFLVERGVPGFSTSDIHGKWSLRASVTSSLS
                     FHDCRIPDANILPGATGLKSPLSCLSQARYGIGWGAVGAAMACYDTARQYAILRKQFH
                     DRPIASHQLVQSELAWMITEITKAQLLALHVGRLKDRGKADFAHISMLKRNNVWMALE
                     TARRSRDLLGANGITDEYPIFRHMCNLETVKTYEGTHNIHTLIIGERITGLPAYD"
     gene            9522..13187
                     /locus_tag="VFA54_10520"
     CDS             9522..13187
                     /locus_tag="VFA54_10520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HZS51286.1"
                     /translation="MDTDVRLLIVAMAGALTAGLAAAGPARSPHSLMLEAYAAFPLNF
                     EPNVGQADSRVQFLARAPGYGLFLTGPETVLLLHGSTGEAVVRWQFIDANNRMRGEGV
                     DPQSATANYFLGSDPAHWRSHIPTYGKVRYKDVYPGIDVIYYANRRQLEYDLVLAAGS
                     DPGRIRLAFPGARNLHVAQNGDLVVETCAGEIRQRRPAVYQESPEGRNPVTGRYVLAG
                     DGVVGLRLGAYDRTRPLVIDPVVVYSTLIGGGANETGAGIAVDSSGNAYVIGTTTSVD
                     FPVTPPIQTAAGGGKDVFIIKFNTSGSGIAYSTYLGGSGDDIGAAIAVDGSGNAYITG
                     NTFSANFPTRNAAQASYGGTSSMSFGDAFAAKIDPAGGSLVYSTYLGGSGDDAGLAVA
                     ADGSGAAYVTGVTSSSNFPASAGAFRGFLSGASDAFVTKLSPAGAIAYSTYLGGGATE
                     QGYGIAVDSGGNAYVTGATASTDFPAVNAFQPQCASSGTPFGVSCADAFLAKLNVSGS
                     GLVYSTYLGGGATDSGRAIAVDASGNAYVAGATNSTNFPVTPNAFQHGFGSGPEDAFL
                     AKFNAAGSALIYGTYLGGTGDDIAYGVAVDAGGAAYVAGSTTSTDFPTAGAVRATCGG
                     SVCADAFVAAINAAGSALIYSTYLGGSGTDRASAIAADSAGGVYVTGETNSSDFLTTA
                     GAYQTSLAGPSDAFVTKLVAGANPTPAITQLTPSSLPPGSPGFNLSVFGTSFIQGSVV
                     RWNGLDRETTFISSGELVAAIPAADVAAAGSAQVTVFNPPPGGGVSNALVFAIGGNPV
                     PVMGTILPATATAGAPGFTLVVLGSAFVPQSVVRWNGSSRTTTFVSSTQLHAAITAAD
                     LAAAGLVQVTVFNPAPGGGVSNAAAFTINDPIPALSSLSPPGAAAGGPALTLAVNGSN
                     FVSSSVVLWNGSARPTKFISGAQLQASIPASDISAPGTSQITVFNPPPGGGLSAALTF
                     TIVAPPVISSGGLVSAASFNSQPQPAGAIVALFGTNLAGATTKASTVPLPTTLGGVTV
                     RLNGVAAPLFFVSPLQINFQIPWELQGQAQASLTVESGGVSSTSMPITLGPFQPAIFT
                     TNMQGSGQGAILLSGTAVVAAPAGALPGSQPAPRSSANRPSFVEIYATGLGAVTNTPP
                     SGSPSPVSPLSTTILPTVTIGSVPATVTFSGLAPGYVGLYQVNVQVPAGAPVGDAVPV
                     VLTIGGVASNTVTMAVQ"
     gene            complement(13236..14087)
                     /locus_tag="VFA54_10525"
     CDS             complement(13236..14087)
                     /locus_tag="VFA54_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZS51287.1"
                     /translation="MKLAQIRNHHGQLTAAIFESGGYRPIDNYTVAALIARAEQQGRP
                     LAETAAALAGKDVLAGVQPDIPLCPPEVWACGCTYAPSAEFRDAELGTREGMYSYVYR
                     AARPEIFFKGAARVCVGPGAPIGIRCDSKFTAPEPELAVVLNGRGEIQGYTLANDVSA
                     WDIERENALYLPQSKIYTGCCALGPALVTPEEIANPYDLQMTCTITRGEETVFRGSVS
                     TSKLFRRFEYLIEYLRRANPVPAATVLLTGTGIIVTEDAALQPGDVVSIEVREIGKLV
                     NPAAVAG"
     gene            complement(14167..15618)
                     /locus_tag="VFA54_10530"
     CDS             complement(14167..15618)
                     /locus_tag="VFA54_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZS51288.1"
                     /translation="MPEVRTYPNYINGEWTSAVRTFENRNPANTEELVGLFAKGGAGD
                     ISSAAEAAQCAFPAWSALAAPARGSYLFKAAEILESRFEQLALEMTREEGKTLPESRG
                     EVRRSINIFRYFAGEGSRMPGHLAPSERDRVFMFSVRKPLGVVGLVTPWNFPSAIPAW
                     KLAPALIAGNTVVIKPASAAPLSAWRLVEALAEAGLPKGVVNLVAGPGGELGEALVNA
                     APLKAISFTGSCGIGQWLHERASRRRLRIQLEMGGKNPTIVLADADFKAAVENTVNGA
                     FFSTGQKCTATSRAIVEDAIYDRFVDALAERTAKLKVGDGREPGVDIGPAIDQNQLDT
                     DLKYIEIGKKEGAKLKFGGRRLTGGAYAKGYFIEPTIFTDVEEGMTIAQEEIFGPVLA
                     VMRARDFDHAIKIANNVAFGLSASLQTQNVSRIFDYVQKIEAGLLTVNLPSAGVEYQL
                     PFGGTKDSSFGPKEQGPAALDFYTDYKTVYLKY"
     gene            15671..16444
                     /locus_tag="VFA54_10535"
     CDS             15671..16444
                     /locus_tag="VFA54_10535"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS51289.1"
                     /translation="MPNHAENPAGAPVCLVTGASSGIGAATAVDFARLQYRVALHYNT
                     NEEGARRTAGAIEKAGGTASLFQADLSQAEPAAGLVAAVLRHFGRIDVLVNNAGSLVA
                     RRRFLDVTGEFWQQVLDVNLNSAFWVTRAAVPHMVERGSGVIVNVGSISGRNGGGPGA
                     IPYATAKAAITGFTKGLAKELAPYGIRVNAVNPGVILTPFHETFSTPERLQAMVATIP
                     QGRAGTAEEVSGVITFLASPAASHIVGESIEVNGGMLVD"
     gene            complement(16533..>17498)
                     /locus_tag="VFA54_10540"
     CDS             complement(16533..>17498)
                     /locus_tag="VFA54_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS51290.1"
                     /translation="AVFVELKRRNVNLGRDVIFLAESGEEGTSDVGVRWMIRNAYDKI
                     NAEFALNEGGFNATLPSGVQLFHIQTSEKVPTRVVLTAHGSAGHGSIPRPDNPIAHIA
                     RALDRLVSTDQPVQLNETTRRYFQAIAKTDDYKWLEPLLAGLENPGTAVATANQIRAK
                     NAELDAALRTTLSPTMLKAGFRVNVIPNSAEAQIDVRRLPAETREEVYNRFRRIMNDP
                     SIDIGPAGLDRPATEPSSMSTALYKTMESVFQKAHPRALVVPYMQHGATDGADLRAKG
                     MAVYGVPLFDESENRLHGNDERIGVANFEKGAGLLWQIVTAVASE"
BASE COUNT         3211 a         5394 c         5510 g         3383 t
ORIGIN      
        1 cgagcgatac cggcgtcatc ccggcggtcg aggtgcactg gccggagaaa ggtcagaccg
       61 agtggctgcg ccgcctgctg accaccctgg cgagagctca gttccacgga ccgtcccccg
      121 cccgaagcga gatccagcgc cggctggcgc gaagcccggt agaagtcgcg gaacaattga
      181 gccggcgcta tggccacgat ttgaatgacg tggcctatca gcctgcctta gctttgatcg
      241 ggaggatctg gctgggggaa ctgaccaagg atccgcagca gctcgccgat gtcgagaaca
      301 ttgtagcgcc gtatttcagc ggagcgaaaa agacgattcc caagaaggcc tcgagcgtga
      361 actttgccgg gcatttggtt tttggcgaat tggcgcgggc aaccgggaag tcccgctatg
      421 tcaacttggc tcgggaagcg gccgattacg ggttcgatga gcacggagcg cccaaggaag
      481 cgctgccagg ccacaatgaa atgagcgacg cggttttcat gggaggaccg atcgaggcgc
      541 aggtggggcg gcttacaggt gaagccaagt attacgatat gagtttgcgc aatatgcgtt
      601 tcctgctcgc gctcgatctg cgttccgatg gcctctaccg gcactcaccc ctcaaccagg
      661 cggcttgggg acgagggaat ggctttcccg ccctcggctt ggccttgagt ttgtcgtatt
      721 tcccaccgga acaggccggc cgggaagaaa tcctgcgcgc tttccgcgcc cacatggccg
      781 ctctcgcgcg ttttcaggac cctacaggcg catggcacga ggtgatcgac gttccgggga
      841 gttatcggga gctgacggcc acctgtatga tcggattttc catgctccga ggggtgcggt
      901 cggggtggct tgaaaaggat cgctacctgc cgctcgccga aaaagcttgg cacgccgtgc
      961 ggacacgcgt ggcgcccgat ggcggattgg tcgatgtgtg tatatccacc ggcaaacagc
     1021 cgagcttgcg cgcttacctg gaccggacag ccatcctggg accggaccaa cgcggcggag
     1081 cgatggctct tctgatcgcg acggaagtgg cccgctggca ggcggaaaat cccggctggc
     1141 gcccctaagt gattggcctc aaagagggtg gaaaaattgt ttgcaaaggg caaaacaccg
     1201 gactaagatg gctgagtttt tcctggaagc aatgcgtagt tcagccctca aggaagcagg
     1261 ttctgccggt gctttattgc tattttatcg ctcgctcggg accagaactc caaaaggttt
     1321 cgtatcgtac ggtccacccg ggtccgatct gctttgtgca acagcacgat ctgctgacgg
     1381 cggttcgtgc actgcccccg gggagccgct ccacgctccc gctcatgctg gagcacaact
     1441 tggtcctgtc ggccgcctct gaacaagcgg ccatcttgcc catgcctttc ggtaccgctg
     1501 tgtcggcgga agaaacgctg gcgcaactcc tcgaggctcg gcgcgcggaa attctggagg
     1561 ctttagatcg tttaggcggt aaggcggaaa tggctctgcg gattacgctc cgtccagacg
     1621 aagatccgca agacagggcc gggcagatcg ccggaatttg ccagccttta cagcagtggt
     1681 tcgaggtgcg cccggacgga agcggggagg cggttctgga gattgctcac ctgatacacc
     1741 gccaggaggc cgatcagtat cggaagcggc tggaaacgca ggccgccaag gtgaacggcc
     1801 cccggcctcc cttccatttt ctcccgccat ttttgcgcaa gccggtgaaa tcggagtcgc
     1861 gccccggaag agcggtctgc cggcgcgacg ccacaggctg attttttact ggatgcggta
     1921 cttctccgcc atcagtcttt gaaagcgggg acgatagatc agatcgtaag caagttcttt
     1981 cccgggaaac agattcaaag cggcgcgcct ggccgaagcg atcaattcgc tggcctcctc
     2041 gatcgacatg gcgtcgtttt ggctgataac gctcatgacc agatccatca tcaattgcaa
     2101 ccgccgtacc ttacgcgctt cgtcggcgat ttcttctgga gttgctgcgg gggtagccat
     2161 taaaagaatt ataataaaaa ccaatgaagc gccgcccgaa agctgcgaag cggggcatcc
     2221 tgcccagggc gcgtgagcac aagccgcatc gaactgcgtt ccggagaacc ccatgaaccc
     2281 cgagatggat caagacaatg aaaccctggt caatgtgttc gaggccgccg gggtgtctgc
     2341 cgagatggag gcgcaagccg tgcgagctct attggatgcg aacggcattg aggccgtggt
     2401 cgtgcgcgaa aatgtcccgg aactgcctgt tgggaaggtc gagattcgcg tggttgcttc
     2461 cgatgccgag cgagccaggg aagtcattcg cgaaggccag ttggccggcc ctgcggcggc
     2521 cgccgaggcc gaagccgagt cggaactgtg aggcgattcc ggttcgggct ggcggccgga
     2581 cgatgggcaa tgagagatca ggccggccgc aggctctttc ccagaccgat ggcgagggcg
     2641 ggtttggagt ccagggccgc cgcccgtaga tgatccctgg cctcccggcg gtgagacatt
     2701 gcctgcatcg aaatttgttc gctgacgcaa gatctccgcg gtttgcgccc ctgtgtcaca
     2761 cttcccctat gagagcggaa caagaaaccc ggccctgggc gctggtttgg gtcggggcgg
     2821 gcgcgagcct ttggggaacg gacaccttct tccgccgtcc cttggcgatg agcctgagca
     2881 gcgcccagat cgtgctctgc gagcacctga ttctaagcgt cgtcttgctg ccggtttttt
     2941 ggcaggcgcg ctcccaatgg cgcgcgctgc ggctatctca atggggcgcg gtcctgggaa
     3001 ttgcctgggg cggttcggcg ctggggacga tctgctttac cgaggcgatc aagctcggca
     3061 atcctaccgc tgcggttttc ctacagaaga cgcagccctt gttcgcggcc tttttggctg
     3121 ccctgctgct tggagagagg ctccaggggc gattctggat ctgcctcctt ttcaccgtcg
     3181 ctggggccta tctggttagt tttggggaga gccccgcttt tttcgccaat ccggcggtcc
     3241 tggctccggg ccgaacggcg gccgcgctgc tggccttggg agcggcagcg ctgtggggtt
     3301 cgtcaaccgt tttcggccgt ttcgcactac aaagcgtgcc attccttact ctgaccgcgc
     3361 tgcggattct ttgcgccacg ccgcttctgg cggcgttcgc ttggctgggc cgccgccaga
     3421 tcgaggtcca cctccagaca aggcaatggc tgtatctggc gctcatggcc ctcgtgcccg
     3481 gactgctcgg actgctggtt tactatcgcg ggctgcgcta cacgagggcc tccctggcgg
     3541 cgctcgccga actcagcttt ccggcgaccg ctgcgctgct gaactggatc tttctgagaa
     3601 cccggatcac cgcgatccaa gccggcggat tcctgctggt ctggctggtt atcgtatacc
     3661 tcgaccggag cgcgcgccgc gacaaaggaa attcagccgc tgtggcggcg gcttagcgat
     3721 tcggcgccgt cgcgcgggtg attgacaact gaatcaatcc ctgtcattgc atgagtccct
     3781 gcactccgtg aaccgacaat tgccaggatg ggaagccctg ggctcgacgg cgccatcgcc
     3841 aggttgcggc cctgcaccag cagttgtacg cgctcctgaa atgctgatgg attttccctc
     3901 tcgggccgcc tcgtttcgcg gagcattgat tccattgctc aggtcacgtt tccgctggat
     3961 tgcgggcgag atcctgcaag agaaggggcg gcctgggctg gttccgttgc gtctacatcc
     4021 gccgtagtcc gcttccaggg ctgtcgggaa agcatgcgct gcggtagaag gcgcgaggcc
     4081 tattgtttca agccgaagga gtacacgcgg gaatcgaacg tggtgacgta aattcttccg
     4141 ctggccagag ccaggccgct gaaatgagtc cagctatcaa tgatgttctc gctggaataa
     4201 agctgtttcc cggtggcggc gtcgaacgcg tagagaatcg cgttgccggt tctggtgtcg
     4261 atgcgttgct tggaagtgag catcaccccg tcttcgccca cttggcgggc gttctcgcca
     4321 ttggccaggg caaagaccac gccgttagcg atgacgggcg gctcgggcat gctcatgtcg
     4381 cgggaaatcc acaccggcgc aagcgcgggc ttgtcgtttt gaacggtaac ctgaaaggcc
     4441 atgatgctgc cgcggggggc ttcgccgtac gtatattgga atttcggcgc gtccttcgac
     4501 ggcggtcccc aggttggcac atagagccag cgctggcctt cgctatcctg ccaggtggag
     4561 atcgcgcccc atacgccgcg gccggcgaag tcgatatcgt cattggaata ccgcgcactg
     4621 aacagcggcg tgcgatgatc ggccccgccg agcgcaagcg cgtcgagcag gtaaataacg
     4681 ccttccttgc cggcggaggc caccaactgc catttttgga acggaaagat caccgggctc
     4741 gcgcaaccca tgtccaggtc ttttttggat acgaactccc gattccgcgg cgtgaagtaa
     4801 tctacgagat tcagcgtctt cggttcgagc gccagaaccg tgtcggccca gcgcccggtg
     4861 gccggatccc acgagccgtc gcccgtttgg acgtagacgc ccttggggcc gaaggcggcc
     4921 ccggcgcgcc cccaaatacc ggccgggccg ccgatgctgg tatagaaatg ggtcacgggg
     4981 cgcgacggat tgctgacgtc cattgccgcc acgccggacc gggcctctcc gcagccctgg
     5041 gaaatcgtgg tgtaaatgac tccattgacc aggttcagac tccagttttt ggagaacggc
     5101 gggacgaaat ccgtgggcgg aaaacgatct tctccattga cgatgttcag gccgcgcagc
     5161 ttgccgtcgc tggtgatcag gtagatggtt cgggactgct tgtcaatgac cggagtggcg
     5221 ttcagcgact cggggcataa ccagcggggc gggagtttgg gcttgagctc gttggtgaat
     5281 ctcttttgcc acagcacctt gcctgtgtcc gaatcgagag cgtaaagatt atcggagctg
     5341 cccgccacga aaaccaggtc cttgaaaccg cggggggtga tcacgttttc aaccaccagg
     5401 ggcgcggtca aagaagccat ctcggtggga acattatcaa gctggctctt ccacttcagt
     5461 tcgaggcgcg aggcgttgcc tttattcagc tccacttcct gcgtcgccca gcccgtccgc
     5521 tccgggttgc cgccaaaagt cagccagtct cgctgcggcc gcgcctgcgc gccgagaagc
     5581 gaaggccaca gcgccaaaag caaagtcacg caaactaaat acagtgccat ctcggaagac
     5641 ctcccgattc cggtattcac tgcgcggccg cgggctccgg ccgggacttc accgcgcagg
     5701 ctgccgctcc gccatgccct cccatcggcg caccaaatcc gtctcgatgg aaaacagatc
     5761 aaggatgcgg ccgactgtgt gattcacgat ttcatcgatc gttttcggat gattgtaaaa
     5821 agcgggaact gggggaacga tcaccgcgcc catttccgcg caggccgcca tattgcggat
     5881 atgagccagc gtcagcggcg cttcgcgcac gagcaaggcc aggcgccggc gctctttcaa
     5941 ggtgacatcc gccgcccgcg tgattagatt gtccgcgtag ccttgggcga tggcggccag
     6001 cgttttcacc gagcagggcg cgatgatcat gccggcggtc tgaaaggacc cgctcgacaa
     6061 cgcagctccc acatcgccca cggcatgacg aaatgccgcc agccgcccaa cctgttcggc
     6121 ggtataggat gtttcccaag caatggtgcg ctcggcagcc ttcgagatca ccacgtgcgt
     6181 ctcgatctgc gccgcgttgc gcaacacctc aagcagccgg atgccataaa ttgctccggt
     6241 cgccccgcag atacccacca ccagccgcgt tgttggcaag ccttattcct tttccatgac
     6301 tttcgcgccc aacgctccgc cggccacggc gagcgaaacg atcatcacaa atccaaaaat
     6361 aatgtagctc agcaggaaga tcatgatgcc caccggggat gccagcaggt cgcgcacctg
     6421 ctgggcgacg gaatcctgcg cgggcattct ttccaccgac tggcggataa tttcgcggat
     6481 gcctccggcg ctgcgagcga gtaggaagct gaaagcggtg agaaacaggc cgatcacgaa
     6541 agtcaaaagt cccgtcagcc agcccattct ggcgccgtcg gctaccgaga gcaggaagcc
     6601 ggtgcgccgg gtatagagca cggtggaaac gaagcctgcg cctaaaatcc agaggcagca
     6661 tccgaagctg atgaatggaa tgacgttaag cagagcactg agcgaggcgc acagcaaggc
     6721 cacgcgcacc gcgatcgggt tcccaaaatt gaccggcggc gccgggggag cctcccgcgc
     6781 cggcccctgg ggcaggcctt cggaatgggc cagcgaggcg ccgagatcct gttcccgttg
     6841 aggcttcccg caatgaaagc aaaagcgggc gttgtcgggc agctcagtcc cgcaggtaca
     6901 agaaaagggc atgaaaaatg ggcatcgtcg ccccgccaaa acctcagggg gcaactgctt
     6961 cagtttgcct caggcgctcc gatattggca agggaaatgc gggttttgcc acgttgggca
     7021 gcgatgccga tcaccttttt gatgccgtcg cggctggagt ggaactcggc acgcccgcgc
     7081 gactcgaaac gcaaagcgaa atcgctcgcc tcgctccggt aaaacgaaac gactttttcc
     7141 atcggatcgg gcgtctccat ctggaccgcc gccacgcgga gggacttgcc ggcaaagtcg
     7201 aggtccacat cgactcgagc ccctccggct tcgccttcta ccaccaccgc ccctgggtac
     7261 agcggaatgc caagaagttt ggggtccacc cgctcctgct tgtcaacccg tacgctgccg
     7321 agaggcgttt cgatgcgaat cgcggtgttt tcgccgcgtc cgcccggcgt ccgggcgacg
     7381 tgaatcctag agacaaagag ataactggcc atgcccaacc cggcgagcgt caacacgaca
     7441 atacctgcga gtacggcggc cacggtcgcc accaggcggc cggtcgatgg ttccgcagag
     7501 gagggcggtg gcagaggcgc agaagagccg ttcatgaggc ggtttcaccc agctttagca
     7561 tacgaagcac agggcggacg cgttccgtcc agaggatgga aatcaggctg aacccaacgg
     7621 ccagaccggt aaggaagcgg ataaccccgg ggactgcggg ccaaatagcg gtctcaacca
     7681 gccactcgcc tcccatgaga cttgtagaag ccaggaacag gcgccgggag aacctgtggc
     7741 caatcagtcc gcctaccagc gcgcccaggt agaatccgaa acagcgcacg cagatagcca
     7801 ggggacggcc gtcgagactc caactccgga ggggctgttg atggcaggcc catgaaaaag
     7861 ccgtgtagac acccagcgaa agcggcgagg cggctctggc gagcagggga gcggaaacgg
     7921 cggccaacag cacgagcgcc ggcgacgtta cggcaaccca gcggatcgaa ttcccactac
     7981 agccgcgcca cacaagctct aatcatacgc cggcaggccc gtaattcgct cgccgattat
     8041 gagggtgtga atattgtgag tgccctcgta ggtcttcacc gtttccaggt tgcacatgtg
     8101 ccggaagatt gggtattcat ccgtaatgcc gttggccccg agcaggtcgc ggctgcggcg
     8161 agccgtttcg agagccatcc acacattgtt gcgcttgagc atcgatatat gcgcgaagtc
     8221 cgctttcccc cggtccttca ggcgccccac gtgcagcgcc agaagctggg ccttggtgat
     8281 ctccgtaatc atccaggcca gttcgctctg gaccaactgg tggctggcaa tcggccggtc
     8341 gtgaaattgt ttgcgcagga tggcgtactg ccgcgccgta tcgtagcaag ccatcgccgc
     8401 gccgacagcc ccccaaccga tcccataccg cgcctgagac aagcaggaaa gcgggctttt
     8461 caggcctgtt gcgcccggaa ggatgttcgc gtcgggaatc cggcagtcat ggaacgaaag
     8521 gctggaggtg accgaagcgc gcagggacca tttgccgtgg atatcggaag tggaaaaacc
     8581 gggaacgccg cgctccacca gaaatccccg gatgactccg tcgagccgcg cccaaatcac
     8641 agctacgtcg gcgaggctgc cgttggtaat ccaggttttt tcgccgttca ggatgtagcc
     8701 cggcccgtcg cgcaccgccg tggtccgcat accagcggga ttggatccga agtcgggttc
     8761 ggtaaggccg aaacatccga tggctgttcc gttttgcagg cggggcagcc agcgctgttt
     8821 ctgctcctcg ctgccgtaag tcaaaatcgg gtacatgacg agcgcaccct ggaccgagca
     8881 aaaactccgc aagctgctgt cgccccgctc cagttcctgc atgatgagac cgtactcgac
     8941 gttgctcatc cccgcgcagc cgtagccggt gagattggct cctaagaatc cgagctccgc
     9001 catttccggc acgaggtgca gcgggaagct gccctcccgg ttgtgttttt caatgatcgg
     9061 gatcacccgc tcttcgacga actggcgcgc cgtccgccgg accagcagtt cctgctcgct
     9121 gaacagggag tcgatttcca ggtaatccac gccccggaag ggaaacatag agtctcattc
     9181 tagcgaaggc cgcgggtcac cgccgcagcg gtcttctgcc cctccggagc ttccggaaag
     9241 ggttcgccgg ctcggggcga agcgcccttg cagtgtaatt caggcgacga aagtcgagag
     9301 agccgggcgc ctccgccgct tcgacgtagc gctcaggctg gcctccgcac gagagcgggc
     9361 cgacgcagcg aaagctacgg cgaaaaaatt gcactggcca tgctgcgggc tctttggcgc
     9421 ccactgccag cgagataaat gcctgcaaat tcaagcgatg cggcataaga ggatttgttt
     9481 ttgggtttga gacagcaata tttccacata tcctgggaat tatggacaca gatgtgcgct
     9541 tgctgattgt cgccatggcc ggagccctga cggccgggct ggctgctgcg ggccccgcgc
     9601 gttcccccca ttcattgatg ctggaagcgt acgccgcctt tcctttgaat ttcgaaccaa
     9661 atgtgggaca agcagacagc cgcgttcagt tccttgcccg cgcgccggga tacggactgt
     9721 tcctgaccgg cccggagact gtactgctcc tgcacggttc aactggcgaa gcggtggtac
     9781 gctggcaatt cattgacgcc aacaaccgga tgcgcggcga aggcgtcgat ccccagtccg
     9841 cgaccgccaa ttattttctc ggcagcgatc cggcgcactg gcgaagccat attccaacct
     9901 atggcaaagt ccgatacaag gatgtctatc cgggtatcga tgtaatttac tacgcgaacc
     9961 ggcggcagct tgaatacgac ttggtgctgg cggccgggtc ggaccccggt cggattcgcc
    10021 tcgcgtttcc aggagcgcga aacctccatg tggcgcaaaa cggcgatctg gttgtcgaaa
    10081 cctgcgccgg cgaaatccgc cagcggcggc cggccgtgta tcaggagtcg ccggaagggc
    10141 gaaatcctgt gacagggcgt tacgtcctcg ccggcgatgg cgtggtgggc cttcgcttag
    10201 gcgcttacga ccggacgcgc cccctggtga tcgatccggt ggtcgtttac tccacgctga
    10261 tcggcggcgg cgccaatgag acgggagccg gcattgcagt ggactcgtcg gggaatgcgt
    10321 atgtaattgg gactaccact tcggtggatt ttcccgtcac gccgcccata caaaccgcgg
    10381 cgggaggcgg caaagacgtc ttcatcatca agttcaatac ttcgggttca ggcatcgcct
    10441 actcgactta cttgggggga agcggcgatg atattggggc cgcgatcgcg gtcgacggct
    10501 ccggaaacgc ctacatcaca gggaacacct tctcggccaa tttcccgacg cgaaacgccg
    10561 cgcaggcctc ttatggcgga acatcttcga tgagtttcgg ggatgcgttc gccgcgaaaa
    10621 tcgatcccgc gggcggatct cttgtgtatt cgacgtactt aggaggtagt ggggacgatg
    10681 ccggattggc ggtcgccgca gacggttcgg gagccgccta cgtcacaggc gtgacctcct
    10741 ccagcaactt ccccgccagc gccggcgcgt ttcgggggtt tcttagcggc gcgtccgacg
    10801 ccttcgtgac gaaactcagc cccgccggcg caatcgctta ttcgacttat ctgggcggcg
    10861 gcgcgaccga gcagggctac ggcatcgccg tggactccgg cggaaacgcc tatgtgaccg
    10921 gagccacggc ttccaccgat tttccggccg tgaatgcgtt tcagccgcag tgcgcctcga
    10981 gcggtacgcc cttcggagtc tcttgcgctg acgcgttcct cgccaagctc aatgtctccg
    11041 gaagcgggtt ggtttattcc acatatctgg gcggcggcgc gaccgactcg ggaagagcga
    11101 ttgcggtgga tgcatccgga aacgcctatg tggccggcgc caccaattcc accaactttc
    11161 ccgtgacgcc caatgccttc cagcatggct ttggcagcgg gccggaagac gctttcctcg
    11221 ccaagttcaa tgccgccggg tcggcgctga tctatggcac gtacctgggc gggacgggcg
    11281 acgacatcgc gtacggtgtc gccgtggatg ccgggggcgc cgcttacgtg gccggatcaa
    11341 ctacatccac cgacttcccg acggctggcg cggtccgggc gacgtgcgga ggatctgttt
    11401 gcgcggatgc gtttgttgcc gctatcaatg cagcgggcag cgccctcatc tattccacct
    11461 atctgggcgg ctcgggaacg gatcgggcgt cagccatcgc ggcggattcc gcgggtggcg
    11521 tctacgtcac gggagagacg aactcctccg attttttgac cactgccggg gcctaccaga
    11581 cgagcctggc cggccccagt gacgcgtttg tgaccaagct tgtggccggc gccaatccca
    11641 cgccggcgat cacgcagtta acgccctcca gtttgccgcc ggggagcccg ggtttcaatc
    11701 tctccgtgtt tggaaccagc tttatccaag gatcggtcgt gcgctggaac ggcctggatc
    11761 gtgaaaccac tttcatcagt tccggtgaac tggtcgctgc catcccagcc gcggatgtcg
    11821 ccgcagccgg ttcggcgcaa gtcacagtgt tcaacccgcc gccgggcggc ggtgtttcca
    11881 acgccctggt gttcgccatc ggcggaaatc cggtcccggt gatgggaacg atactccccg
    11941 ccactgccac ggccggcgct cccgggttca ccctggtcgt tctaggcagc gcgtttgttc
    12001 cacagtccgt ggtgcgctgg aacggaagca gccggacgac aacttttgtg agcagcacgc
    12061 aactacacgc tgccatcacc gcggcggacc tggcggctgc gggcctcgtg caagtcaccg
    12121 tcttcaaccc tgcgccgggg ggcggggtgt ccaacgcggc ggctttcaca atcaacgacc
    12181 cgatccctgc tctttcctcg ctttcgcccc ccggagcggc cgcgggcggc ccggcgctga
    12241 cgctggcggt gaacggctcg aacttcgtct cgagctcggt cgttctttgg aacggcagtg
    12301 cgcggccgac gaagtttatc agcggcgcgc agttgcaggc ctctattccg gcgagcgaca
    12361 ttagcgctcc gggaacatcc cagattacgg ttttcaatcc gccgccgggc ggcggcctgt
    12421 cggcggcctt gacattcacc attgttgcgc cgccggtgat ctcttcgggc ggcttggtga
    12481 gtgcagccag cttcaacagc cagccgcagc cggccggcgc gattgtggct ttgttcggaa
    12541 ccaatctcgc tggcgccacc acgaaagcgt cgaccgtacc gctgccaacc acgctcggcg
    12601 gcgtcaccgt gcggctgaac ggagtggccg cgccactatt cttcgtttcg ccgctgcaaa
    12661 ttaattttca gattccgtgg gaattgcaag ggcaagcgca ggcgtcgctc accgtcgagt
    12721 ccgggggcgt ttcgagcacc tccatgccga tcacgctggg accgttccag ccggcgattt
    12781 tcaccaccaa catgcaagga tccggccagg gagccattct gctttccgga acggcagtcg
    12841 tggccgcgcc cgccggcgcc ttgcccggat cccagccggc gccccgcagt tctgcgaatc
    12901 gcccctcctt cgtcgagatc tacgccaccg gattgggcgc ggtcactaat acgccgccca
    12961 gcggctcgcc gtctccggtg agtcctctct ctacgacgat tcttcccacc gtcacgattg
    13021 gcagcgtgcc ggcgacagtg actttttccg gactggctcc gggctacgtg ggactttatc
    13081 aggtgaacgt gcaggtgcct gcgggcgctc cggtggggga tgcggtgcct gtcgtgctga
    13141 cgatcggcgg tgtcgcttcc aatactgtga cgatggcggt gcaatgaggg gaagcgcaac
    13201 caagcggcga agcgcggcca acagggatat ctctgtcagc cggcgaccgc cgccggattg
    13261 accagcttgc cgatttcgcg cacctcgatg cttaccacgt ccccgggctg gagcgcggca
    13321 tcttccgtca cgatgatccc ggtgccggtg agcaggacgg tggccgcggg aacgggattc
    13381 gccctccgca ggtactcgat cagatactca aagcggcgaa acagcttgga tgtcgagacg
    13441 gatccccgga aaacggtttc ctcgccgcgc gtgatggtgc acgtcatctg caagtcgtac
    13501 ggattggcaa tttcctccgg cgttaccagc gccgggccga gcgcgcagca gccggtgtag
    13561 atcttggatt ggggcaaata gagcgcgttt tcccgctcga tatcccaggc cgaaacgtcg
    13621 tttgccagcg tatatccctg gatctcaccc cggccgttga ggacgacggc taactccggc
    13681 tcgggcgcag tgaatttcga atcgcagcgg atgccgatag gagcgcccgg cccgacgcag
    13741 acccgggcgg cgcccttgaa aaaaatctcc ggccgcgcgg cccggtagac gtagctgtac
    13801 atgccctcgc gcgtgccgag ctccgcgtcc cgaaactcgg cgctgggggc gtaggtgcag
    13861 ccgcaggccc agacttccgg cgggcagagc ggaatatccg gctgcactcc cgccagcacg
    13921 tcctttccgg ccagcgccgc cgccgtttca gccagcggcc gcccctgctg ctcggcgcgc
    13981 gcgatgagcg ctgcaactgt gtaattgtcg atggggcgat agccgccgct ctcgaaaatg
    14041 gctgcggtga gctgtccgtg atggttccgg atttgggcga gtttcatagt tcagcctggc
    14101 ttatgaggga atttgcccag accaggagaa acggcctgcg attcggccgg gctccgtgtc
    14161 ttgccatcag tacttcaaat acactgtttt gtaatccgta tagaaatcga gcgctgcggg
    14221 cccctgctct ttggggccga agctggaatc tttcgtgcct ccgaagggca actggtactc
    14281 cacgcctgcg ctgggcaggt tgaccgtcag cagaccggct tcgatttttt gcacataatc
    14341 gaagatgcga gaaacgtttt gcgtctgaag ggacgctgaa aggccgaagg cgacgttatt
    14401 ggcaattttg atcgcatggt cgaagtcgcg cgcccgcatt accgcgagca ccgggccgaa
    14461 aatctcctcc tgcgcgatgg tcataccctc ttcgacgtcg gtaaagatcg tgggttcgat
    14521 aaagtagcct ttggcatacg cgccgccggt caggcgccgc ccgccgaatt tcaacttcgc
    14581 gccttctttt ttgccgatct ctatgtactt caaatcagta tcgagctggt tctggtcgat
    14641 cgccggcccg atatcgacgc ccggctcgcg gccatccccg accttcagct tcgccgtgcg
    14701 ctcggccagg gcatcgacga agcggtcgta aatcgcgtct tccacaatgg cgcggctggt
    14761 ggccgtgcac ttctggccgg tggagaagaa agcgccgttc acggtatttt ccacggcggc
    14821 cttgaaatct gcgtcggcga ggacgatggt cgggttcttg cctcccatct caagctggat
    14881 ccgcaagcgc cggcgcgagg cccgctcatg cagccactgg ccgatgccgc aagagccggt
    14941 aaacgaaatc gccttgagag gcgccgcatt cacgagcgcc tcgcccagtt cgccgccggg
    15001 gcccgccacg aggttgacca cgcccttggg caggccggct tcggccagcg cttctaccag
    15061 ccgccaagcg ctcagcggcg ctgcggaagc gggtttgatt actacggtgt tgcccgcgat
    15121 cagcgccggc gccagcttcc aggccgggat ggcgctcgga aaattccacg gcgtcaccaa
    15181 cccgaccact cccagcggct tccgcactga gaacatgaaa acgcggtcgc gctcggaagg
    15241 cgccaggtgg cccggcatac gcgagccttc cccggcgaaa tatcgaaaga tgttgatcga
    15301 gcggcgcacc tcgcccctgg attcgggcag cgttttgcct tcctcgcggg tcatttcgag
    15361 agccagttgc tcgaagcggg attcgagaat ttcggcggcc ttgaacaagt aactgccgcg
    15421 ggcgggagcg gccagcgcgc tccaggccgg gaacgcgcac tgcgccgctt cggcggcgct
    15481 gctgatgtcg ccggcgccgc ctttcgcgaa caggcctacc agttcctcgg tattggccgg
    15541 gttccgattt tcgaacgtgc gtacagcgct cgtccactcg ccattgatgt aattgggata
    15601 agtgcgaact tcgggcatcg gcgttgcctc cggctcgggg aaattctctt tgtactacaa
    15661 ttcgggatct atgccaaacc atgcagagaa ccccgccggc gcgccggttt gcctcgttac
    15721 gggagcttcc agcgggatcg gagcggccac agccgttgat ttcgcccgcc tccagtaccg
    15781 cgtcgccttg cattacaaca ccaacgaaga gggagcgcgg cgtacggccg gggcgatcga
    15841 gaaggccggc gggacggcaa gcctgttcca agcggatctc tcgcaggcag aacccgccgc
    15901 cggcctggta gcagcggtgc ttcgccactt cgggcgcatt gacgtgctgg taaacaacgc
    15961 cgggtcgctc gtggcgcgca ggcgattcct ggatgttacc ggcgaattct ggcagcaggt
    16021 cctcgacgtg aacctcaata gcgccttctg ggtgacgcgg gccgccgtgc cgcacatggt
    16081 cgagcgcggc tcgggtgtca ttgtcaatgt cggttccatc tccgggcgca atggcggtgg
    16141 tccgggagcc attccctatg cgacggcgaa ggcggcgatc accggcttca ccaaggggct
    16201 tgcgaaagag ctcgcccctt acggcatccg cgtcaacgct gtgaaccccg gcgtgattct
    16261 gacgccgttt cacgagacct tcagcactcc ggagcgcttg caggcgatgg tggcgacgat
    16321 tccacaaggc cgggccggga ccgccgagga agtctccggc gtcatcactt tcctggcttc
    16381 cccggccgcc tctcacatcg tgggcgagtc gatcgaggtc aatggcggga tgctggtgga
    16441 ttgaacattt gggcccagag gtctgctgcc tgggaacctc gcagccggcc tcgcatcctg
    16501 aaagctgaag gccgatagcc gaaagctgat agctactcac tcgccacggc cgtcacgatc
    16561 tgccacagca agcctgcccc tttttcaaaa ttggcaacgc cgattcgctc atcgttgccg
    16621 tgcaaccggt tttcgctttc gtcgaacagg ggcacgccgt atacggccat gcctttcgcg
    16681 cgcagatcgg cgccgtcggt ggcgccgtgc tgcatataag ggacgaccag ggctcgcgga
    16741 tgcgcctttt ggaaaacgct ttccatggtt ttatacaacg ccgtgctcat ggagctcggc
    16801 tcggtggccg ggcgatccag gccggcaggg ccgatatcga tcgagggatc attcatgatt
    16861 ctgcggaacc ggttgtagac ctcttcgcgc gtttccgcgg gcaagcggcg aacgtcgatc
    16921 tgcgcctcgg cgctgttagg gattacattg acgcggaagc cggccttgag catggtgggc
    16981 gaaagcgtgg tgcgcagcgc agcatcgagc tccgcgtttt tggcccggat ttgattggcc
    17041 gtggcgacgg ctgtgccggg attttccagt ccggcgagca gcggctccag ccacttatag
    17101 tcatcggtct tggcgatggc ttgaaaatag cgccgcgtgg tttcgttcaa ctgaacgggc
    17161 tggtcggtgg aaacgagccg gtcgagagcg cgggcgatgt gcgctatggg attatcgggg
    17221 cggggaatgg atccgtggcc ggcggagcca tgagcggtca acaccacgcg cgtgggaacc
    17281 ttttcgctcg tctggatgtg aaaaagctgc acgcccgagg gaagggtagc gttgaagccg
    17341 ccttcgttca gggcgaactc ggcgttgatc ttgtcatacg cgttgcggat catccagcgc
    17401 acgcccacgt cggatgttcc ctcttcgcct gattcggcca ggaagatcac gtcgcggccc
    17461 aggttcacgt tgcgccgctt cagctctaca aatacagc
//
LOCUS       DASREK010000204        17497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_263838, whole genome shotgun sequence.
ACCESSION   DASREK010000204 DASREK010000000
VERSION     DASREK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17497
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263838"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VFA54_10545"
     CDS             complement(<1..286)
                     /locus_tag="VFA54_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51291.1"
                     /translation="MRPHGLYNQKGVFCGLKILILFMLAALQTTAFRGRFAVRTPVLL
                     ALSACLWSAVSLCAPAQEFTVELQASSRSQRQTARSSQAAGARPVLPAGAG"
     gene            423..2198
                     /locus_tag="VFA54_10550"
     CDS             423..2198
                     /locus_tag="VFA54_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZS51292.1"
                     /translation="MPQQNNPEVDLRKVYDAIVVGSGAAGGMAAHVLTSHGMQVLLLE
                     AGPKLDIDKELKSMEWPYDHPRRGDMPPGRYALTRIDYRRNPPYAQGSRYDHVHSYVQ
                     SWDFPDYSKNIVVDEKDHPFTGTPFAWVRARCLGGKTNIWGRLSLRLSDYDFKAKTHD
                     GYGEDWPISYADLAPYYDKVDLYLGISGHKENLPQLPDGLFQRSHKLNWAELHLRKTL
                     ASMGRTLTPYRAGVTTDGLKHNKYRSRCFGRGACSRRVGGCDIHAAFDSPTGLIYPAM
                     DTGNLTLRPNSIVHEILVDKNTGKASGVAFLDAESRRSYQARARVVVVGASTLESARL
                     LLLSKSPAYPNGIGNSSGHVGHNLCEHLMGPGVSGIMEDLVGKAPTLDDGRPGGFYIP
                     RFRNLSSRHPNFIRGYGFEGGSGSQMFPGNALSTPGFGAAYKKEVRDYAGASISMSGF
                     GEVLARYENYVDLDPEVKDRWGIPVLRFHYQFGDNEKKMAADMADAAGEMFEAAGFKN
                     IRVRRNILTEGWSIHELGTARMGSDPKTSVLNQFQQSHDVKNLFVVDGSSHVSASCQN
                     PTWTIMALCWRSCDYLADQFKKGNL"
     gene            2198..2704
                     /locus_tag="VFA54_10555"
     CDS             2198..2704
                     /locus_tag="VFA54_10555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZS51293.1"
                     /translation="MADPTRRDALKITAGAVLAAPLRAQGAPRFFTPEEFRMVDELTE
                     MIIPGDGHSPGARAAQCAAYIDLRLAEAFEPETRQQWRDGLKRLDELCRRMCGRGFVE
                     AAGEQRTAVLRHVSAREAPQTPEELFFRELKARTANAYYTSRIGIHAEMQYKGNVAQA
                     EYAGYEVK"
     gene            3207..4784
                     /locus_tag="VFA54_10560"
     CDS             3207..4784
                     /locus_tag="VFA54_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS51294.1"
                     /translation="MNRQIPLLFLLCCFTLSSQEPSWEEATRQGNVFQAEGDYTGAER
                     SYKAALQTAGRFAEDDARLAGSLNNLAALYYRLGRYSDAEPLYQRAVSVGEKAAAPEG
                     SPRRVNLGRALGNLAALERALGRYGEAESLYRRSAEILESALGPADPSVATSWNNLAA
                     LYGVLGKYAEAEQLLQRSLESKRASLGPNDPQVAAGMSNLAELYREEGKYGGAEMLLE
                     GAIGILRANSPVNHPGLASALNNLASVFRHQGRDREAEPLYREAIEIWRNSLGSEHPD
                     LAAGLNNLGQLYAAQRRFNDAEPLYTRAVSIWEKALGPDHPNVAAGLNNLAAMYMAEF
                     RFVQADSIFHRALAIQEKAFGQYHPSVATSLNNLGALYFQGGRYAEAERLYRRALEIR
                     EKTQGPEHPEVAATLVNLARLLQARRKNAQAGPLYRRALAIFEKALPSGQPEMAACLK
                     SYAALLRQARRRVEAEQLEKRAKELLTGGLRSRNAGEPAAGCQSASCRSGRKCALGAE
                     DCTEMRLAAPARRDAIF"
     gene            complement(4781..6019)
                     /locus_tag="VFA54_10565"
     CDS             complement(4781..6019)
                     /locus_tag="VFA54_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HZS51295.1"
                     /translation="MQKQWLVVCFIAAAAGAAAQAPYDLLLKGGHVIDPRNNRNGVMD
                     VAIAAGKIAQIAPNIAPSQAKRVIPAAGLYVVPGLVDIHTHVYAGTGRRDVYCGDLSV
                     YPDGYTFRSGVTTVVDAGTSGWKSFPDFKDRVIDRSKTRILAMLNIVGTGMCGAVEQD
                     TSDMDPEATARVARQYPQLIVGVKTAHYQGPEWTAVERAVRAGQLADIPVMVDFGTFR
                     PERPYQQLVLEKLRAGDISTHMYLGTVPMLDDKGKVLPYLFQARQRGVKFDVGHGAGS
                     FLWRQAAPAIRQGWIPDSISTDLHVRSMNGGMKDMTTTMSKILILGVPFEQVIRMSTI
                     NPALEIKRPDLGHLTPGANADVAVLRLRTGEFGYLDVRNARQAGKQKIECELTIRDGQ
                     VVWDLNGLAGDDWHKLGANY"
     gene            6177..9740
                     /gene="metH"
                     /locus_tag="VFA54_10570"
     CDS             6177..9740
                     /gene="metH"
                     /locus_tag="VFA54_10570"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZS51296.1"
                     /translation="MTSAEHRRLALYEALQARILVLDGAMGTMLQERRLTAADFGGAA
                     LEGCNEYLVKTRPDVVLDIHRAYLDAGSDIIETNSFGGTSIVLGEYEIASQARDLNLA
                     AARLARRAADEFSTPAKPRFVAGSMGPTTKAITVTGGVSFEQLREAYYEQALALVEGG
                     ADLLILETCQDTRNVKAGLLAIARAERESGRKIPVIVSGTIEPMGTMLAGQAADAFWV
                     SISHADLLAVGLNCATGPEFMTDHIRTLSELAWTRVSCYPNAGLPNEEGKYLETPQSL
                     AEQLERFIQHGWINLVGGCCGTTAAHIRAIAQMAEGRPPRVPPQRSRRVFYSGIELVE
                     AEESNRPLLVGERTNVIGSRLFKNLVAEEKWEEATEIARRQVKGGAQIVDVCLQSTDR
                     DEIKDIPPFYEKLIRKIKAPVMIDTTDARAVELALTFCQGKSIINSINLEDGEEKFHR
                     TCPLARAYGAALVVGCIDEDKSQAQAFTRERKLAIAERSYRLLTEKYGIQPEDIIFDP
                     LVFPCATGDANYIGGAVETIEGIRLIKQRIPFVKTVLGISNISFGLPPAAREVVNSVF
                     LYYCTKAGLDLAIVNAEKLERFASIPAGERQLAENLLFNTPPVGTAAPEDWRLQTPEQ
                     KAAINQFHIAAITEHFRKAGAKTGAKAADLPLDERLARYIIEGTKDGLIADLDQKLKE
                     GAAPLDIINGPLMDGMREVGDLFNRNELIVAEVLQSAEAMKAAVSYLEQFMEKAETAA
                     RGKIVLATVKGDVHDIGKNLVEIILSNNGYQVINLGIKVPPQDLIQAYHQYNPDAIGL
                     SGLLVKSAQQMVITAGDLREAGVRVPLLVGGAALSDKFTRRKIAPSYGEAVLYAKDAM
                     TGLNLMNRLMDPREREAVLRAHKSAEEAAVGQEAPAAAIGDGQGVPRIPQTRSRKIRV
                     DIPIPPAPYLDRKVRHLPQLAEIWSYINPQVFYGRHLGFKGNFEKRLAEHDEKALELH
                     ARVEELKQEAARFMKVRAVWQFFEAEREGNSIRLFGPGERNPLYTFHFGRQPREDGLC
                     LSDYILDAANGQRDHLALFVVTAGEGIRDRLEAAKKSGEYFKSIALHALAVETAEAAA
                     EWLHRRIREDWGFPDPPGMTMQERFTSRYRGKRYSFGYGACPNLDDQQGIWKLLRPED
                     IGVHLTEGMMMEPEASVSALVFHHPDCAYFTASEETELVTA"
     gene            9836..10510
                     /locus_tag="VFA54_10575"
     CDS             9836..10510
                     /locus_tag="VFA54_10575"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HZS51297.1"
                     /translation="MERPLPLFLKIVFALLHLLADFLVYWLLFGGGLRAAQRFGLRWE
                     PGDFPRRVLLMGCSAVYFLRTLVTAFVFLKRRMTWSEAATTAVWRLLVHAAFAVSGGT
                     NRRPVGLLAVIGAILYWAGSCLNTGSEFQRHVWKQKPENQGKLYTQGLFRLAIHINYV
                     GDLVLFAGFAMIAGSPYTALIPPLTLAGFVIFNIPGLDRHLCEKYGAAFEEYACRTKK
                     LTPFVY"
     gene            10853..11047
                     /locus_tag="VFA54_10580"
     CDS             10853..11047
                     /locus_tag="VFA54_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51298.1"
                     /translation="MRDGRALAMRFLCEQEGQDTMEYTLLLGFVALAAAAVFPVLSAN
                     IKVIWQIASNHLSNSASAAK"
     gene            11507..13078
                     /locus_tag="VFA54_10585"
     CDS             11507..13078
                     /locus_tag="VFA54_10585"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HZS51299.1"
                     /translation="MRKLILVLLAAAAVCPAQYDVLIRNGRVIDGTGNPWRRTNIAIN
                     GDTIAAIGPLIGATAKRTIDARDQIVSPGFIDIHTHSRRNIEQVPTADNYLRQGVTTL
                     IEGPDGSSELPLRSLFARVSAKGTSVNFGSMVGQGTVRQEVMGLVNRPATPAEIERMK
                     AIVRSEMEAGALGLSTGLFYVPGNFTPTAEVTELARVAGEMGGIYISHMRDESDKLIE
                     SVKETIAIGENAHMPTQITHHKVLGAPYKGESVETLKLVEEARARGVDVTLDQYPYTA
                     SSTGLTGLVPQWAQEGGHDAMVKRFQDPAIRPRIKAGIVENIKIRRGGGDPKNVQLAV
                     CQFDPSLNGKTLAQITQERGKEPTAENSADITMEILSHGNCQAVYHAIDESDIERIMK
                     YPDTMIGSDGEVAVFGRGVIHPRSYGTFARVLGVYVRERKVITLQDAIRKMTSLPAQR
                     MNLMDRGLLRPGMKADVVIFDPNVIADKAEYGNPHQYAVGVSYVLVNGQVAIDDGRIT
                     AARPGRILYGPAHKN"
     gene            complement(13150..14574)
                     /locus_tag="VFA54_10590"
     CDS             complement(13150..14574)
                     /locus_tag="VFA54_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZS51300.1"
                     /translation="MKPVNVTFLALLLGSAPAWSQAVQNAAPENAGRTPIYRITVVGR
                     TIKAVNYEHLSEPTKIDFRGTVLAPQAKGEAVVESKKGATKIDARIERLPDATRFGPE
                     FLSYVLWAITPDGRATNLGEVVPDADGNAKLHVTTRFQAFGLLVTAEPYYSVTQPSDV
                     VVLENAVRPDTQGKIEEVRANAQLLPRREGYTLNAGASQAQPEGKKLSMDRYQAVVAL
                     YQAQNAVQIAQAEHADTYAPDVFQKAQQLLQQAQSYESRKASPKQVITSAREAAQAAE
                     DARTITASKKEEERLLAEKEAALRAAARAKAEAAVAEQQKTAAEREAEEQRARAIRAG
                     EAADQPAAAPADLRTQLFEQLSSLGPTRWTARGLVVDLNDAAFQWNAAGLQPPALDAL
                     AKVVSILRAHPELKVEVEGYTNGTSDQQLMANRAAGIRDYLVQQGIQAASMTARGLTM
                     RPPAGAPQMRHVAIIISGSSLGIS"
     gene            complement(14817..15995)
                     /locus_tag="VFA54_10595"
     CDS             complement(14817..15995)
                     /locus_tag="VFA54_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51301.1"
                     /translation="MKKICLLAVFVSAIAWGQQPGMARFRGRLAWRLENNRLRVTVLQ
                     GGGHIAELVLKNPPGGREINPLWIPPWPSIEPSTYNKDKYGAVYGTDSEAATLSGIMG
                     HNLAFDFWGAPSPSEFQAGLSYHGEVSTLAWYVVTSHESSGNIDFTYRADLPAGRTSM
                     TRTLRLRQGQPVLYFEETAENQNAFDRPIGWVEHVTFGPPFVDANFSAFDASATRGER
                     SLGDRSHEFHWPTDGDRDFRRFSQDQRSADLAYLLLDPRRDVEFLSAVNREYRLVVAY
                     VFRRADFPWLNVWEQNKERMQPPWNGATQTRGMEFGNTRVSGTARAYFQMPALWDTPT
                     FGWLNAKEKRTARYLAVLAPIPDGFEGVRDILIAGNEILIVGRPEEKTIRVPFDPSLF
                     "
     gene            complement(15995..16969)
                     /locus_tag="VFA54_10600"
     CDS             complement(15995..16969)
                     /locus_tag="VFA54_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS51302.1"
                     /translation="MTAPVSIAEGIWLIRLPLPFPVAILNVYLIRTGDGFLLLDCGLK
                     TNACRKALAEALSSIGVPWTDIRRLIITHLHPDHFGLAAEVQNRSGAGIWVHAAEAAL
                     LSPRFMDNDLFSEQSAWLTENGAPRDETEEIAQASRGISEFIDMFSPEGFLEDGDRIP
                     IHGGEIETLLTPGHSPGLLTFYFAGRRLYFSSDHMVEKITPNIGLHGHSSGNPLGDYL
                     ASLERVRELDIDWVLPSHGQPFRNHRDWICATMLHHEARCRRMHSAVAATPRTAYEVA
                     AAEWGRRLSPLNERFAMAEALAHLEYLRRQGQVAAVRDGNLIRWRAVS"
     gene            complement(17049..>17497)
                     /locus_tag="VFA54_10605"
     CDS             complement(17049..>17497)
                     /locus_tag="VFA54_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51303.1"
                     /translation="AFAISLYLQLGVGWDSDDPIGFAKLMLVTVAATTLVWVAVTLLT
                     KPEPEETLVAFYRRARPSPVLWGPVAAKAGVPAADDLAGSFRNWFAGCVLIYSSLFGI
                     GKLLLHEWGEGLLFVSVAAGSTWALARSRAGEAALAEQPLDAGGLK"
BASE COUNT         3477 a         5295 c         5349 g         3376 t
ORIGIN      
        1 cccccgcgcc ggccgggagc acgggccgcg cccctgccgc ctgagagctt cgggcggttt
       61 gcctctggct gcgagagctt gcttgcagct ccacggtaaa ttcctgcgcc ggggcgcaaa
      121 gggaaacggc cgaccacagg caagcagaca gcgcgaggag gaccggcgta cgcacggcga
      181 atcgtccccg gaaagctgtg gtctgtaaag ccgccagcat gaacaaaata agaattttca
      241 ggccacaaaa aacgcccttt tgattataca gcccgtgcgg ccgcacgggt attctcctcc
      301 cagagagccc tggggaaatt tgagccggcg gccggagttt aacgggtttt ttggtgctcc
      361 cattttccca agttactgac cggccgctgc cctaccggag tatcataggg ggcgtcacgc
      421 ttatgcccca acagaacaat cccgaagtgg acctccgaaa agtctacgat gccattgtgg
      481 ttggctccgg agccgccggc ggcatggccg ctcacgttct cacctcgcac ggcatgcaag
      541 tgttgctgct tgaagcgggg ccgaagctcg atatcgataa ggaattgaaa tccatggagt
      601 ggccgtacga ccatccgcgg cgtggcgaca tgccgccggg acgctacgcc ctcacgcgca
      661 tcgactatcg ccgcaatccg ccgtacgcgc aaggatcccg ctacgatcac gtgcattcct
      721 acgtccagag ctgggatttc ccggactaca gcaagaatat cgtggtcgat gaaaaggatc
      781 atccattcac cggaacgccg ttcgcctggg tgcgcgcgcg gtgcctcggc ggcaagacca
      841 atatctgggg ccggctttca ctgcgcctct cggattacga tttcaaagcc aaaacccacg
      901 acggttacgg ggaagactgg cccatttctt atgccgacct tgccccttat tacgacaaag
      961 tggatctgta tctggggatc tccggacaca aggaaaatct gccccaattg cccgacgggc
     1021 tttttcagag gtcgcacaag ctgaattggg cggagttgca cctgcgcaaa acgctggcct
     1081 cgatgggccg cacgctcacg ccctaccggg cgggtgtgac taccgatggt ctcaagcaca
     1141 acaagtatcg cagccgctgc ttcgggcgag gcgcctgctc ccggcgggtc ggcggctgcg
     1201 atattcacgc cgccttcgac tcgccgaccg gcctgattta tccagcgatg gataccggca
     1261 atctcacgtt gcggcccaat tccattgtcc acgagattct tgtggacaaa aacaccggca
     1321 aggcgagcgg cgtagctttc ctcgacgcgg aaagccgaag aagctatcag gccagggcca
     1381 gggtcgtggt cgtaggcgct tcgacgctgg agtcggcccg cctgctgctg ctgtccaaat
     1441 ccccggccta cccgaatggc atcggcaact cgagcggcca tgtgggccac aatctgtgcg
     1501 aacacttgat ggggcccggc gtcagcggga tcatggaaga cctggtcggc aaagctccca
     1561 ctctcgacga cgggcgtccg ggaggctttt atattccgcg cttccgcaat ctttcatcgc
     1621 ggcatccgaa tttcatacgc ggctacggat tcgagggcgg ttccggcagc caaatgtttc
     1681 ccggcaatgc tctttccacg cccggctttg gggcagccta caaaaaagag gtgcgcgatt
     1741 acgccggggc ttcgatcagc atgagcggat tcggcgaggt tctcgcccgc tacgaaaact
     1801 atgtggatct cgacccggag gtaaaggacc gctggggcat tccggtgctc cgctttcatt
     1861 accagttcgg cgacaacgag aaaaaaatgg ctgccgacat ggccgacgcc gcgggcgaaa
     1921 tgttcgaagc ggcgggattt aaaaacattc gcgtccggcg caacattttg accgagggct
     1981 ggtcgatcca cgagcttgga acggcccgca tggggtccga ccccaagact tccgtgctca
     2041 accagtttca acaatcgcac gatgtgaaaa atcttttcgt ggtcgatggc agcagccatg
     2101 tgagcgcttc ctgccaaaat ccgacgtgga ccatcatggc tttgtgctgg cggtcgtgcg
     2161 actacctggc cgatcagttc aagaaaggaa acctataatg gcggatccga cgcggcgaga
     2221 cgccctcaaa attacagccg gggctgtgct ggcggcccca ctccgggcgc aaggcgcgcc
     2281 ccgcttcttc actccggaag aattccggat ggtggacgag cttaccgaga tgatcattcc
     2341 gggtgacggc cattctccag gagcccgggc ggcgcaatgc gcggcctaca tcgatctacg
     2401 cctggcggaa gcgtttgagc cggaaacccg gcagcagtgg cgagacggcc tgaagcggct
     2461 ggacgagttg tgccgtcgca tgtgcgggcg cggttttgtg gaggccgccg gggagcagcg
     2521 cacggccgtg ctccggcatg tctcggcaag agaagccccg caaacccccg aggagctttt
     2581 cttcagggag ctcaaggcgc gcacggcgaa cgcctactac acctcgagaa tcggaattca
     2641 cgccgaaatg caatacaagg gcaatgtggc acaggcggaa tatgccggct atgaagtcaa
     2701 ataacgcgta ccggcgcgtg gcccgtgaag ccggtctgcg ccgggtaatt ctgcgtcctg
     2761 gacttcggct cttccactcc tgatgagcgc atcgcgaaag attcagcagc cgcggctgat
     2821 tcagacgttt cggcttcctc ccgcatatct ccgggcttgc aggtttcgcc cttactgcta
     2881 agagcgcacc ggcgccttac ccattcacac tctctcgccg gcggcaaatt cttgcattcc
     2941 gtctggtttt ggcgtcatgc cggaaaagtt gggcggcatc gcgaatgccc gctcacgttc
     3001 gcaggttttt tcatgatgcg aatcgcacgg ccagggaggg ccagagcgca gccggtgagg
     3061 gaagccagct actcctgcgt cttaagacgt acctaagaag cgtccggtct tccgggtggc
     3121 gccggatgtt aacttttgca attcggagtg accagttggc ggctttcgca gcaggttttt
     3181 tcaaggcccg aggagatagt tttcgaatga accgccagat accgctcctg tttctcctct
     3241 gctgtttcac tctttcttcg caagagccgt cctgggagga agccacgcgg cagggaaacg
     3301 tattccaggc cgaaggcgac tataccggcg ccgagcggag ctacaaggct gcgctccaga
     3361 cggccggccg attcgctgaa gacgatgccc gcctcgccgg cagtttgaac aatctggcgg
     3421 ctctctatta ccggttagga cggtacagcg atgcggagcc gctatatcag cgcgcggtat
     3481 cggttggcga gaaagcggcc gctcccgagg ggtcgccgcg acgcgtaaat ctcggcagag
     3541 cactcggcaa tctggctgcg ctcgagcgcg ctttaggccg gtacggagag gccgagtctc
     3601 tctaccgccg ttcggctgag attctggaga gcgccctcgg accggccgac cccagcgtcg
     3661 ccacgagctg gaacaatctg gcagcgcttt atggcgtgct cggcaaatat gccgaagcgg
     3721 agcagcttct acaacgttct ttggagagca agcgggccag cctgggcccc aacgatcccc
     3781 aggtggccgc cggcatgagc aacctggcgg aactgtacag ggaagaggga aagtacggcg
     3841 gagcggaaat gctgttggaa ggtgcgatcg gaatactgcg ggccaattcg ccggtaaatc
     3901 atcccgggct ggccagcgct ctaaacaatc tggcctcagt tttccggcat caaggccgcg
     3961 atcgcgaagc cgagccgctg taccgcgagg cgattgaaat ctggcggaat tctctgggaa
     4021 gcgagcatcc ggacctcgcc gccggcttga acaatttagg gcagctttac gcggcccaac
     4081 ggcgctttaa cgacgccgaa ccgctgtata cacgcgccgt ttcgatctgg gagaaggcgc
     4141 tcggcccgga tcatccgaat gtcgccgcgg ggctgaacaa tctggcggcg atgtatatgg
     4201 ccgagtttcg tttcgtgcag gctgattcga tctttcaccg ggctcttgcc attcaggaaa
     4261 aggctttcgg ccagtaccac ccgagcgtcg caaccagcct gaacaatctg ggggcgctct
     4321 attttcaagg cggccggtac gcggaggcgg aacggctcta tcgccgcgcg ctcgaaattc
     4381 gcgaaaaaac acaagggccc gaacatcctg aggtggcggc cacgctcgtt aacctggccc
     4441 gattgctgca agcccggcgc aagaatgcgc aagccgggcc gctttatcga agggcgctgg
     4501 cgatattcga aaaagctctt ccctccggtc agcccgagat ggcggcgtgc ctgaaatctt
     4561 atgccgcgct gctacgtcag gccaggcgca gggtggaggc tgagcaattg gaaaaaagag
     4621 ccaaggagtt gctgacggga ggcttgcgct cgcgcaatgc cggcgagccg gctgccggtt
     4681 gccagtcggc ttcttgcagg tccggccgaa aatgcgcgct tggggccgag gattgtacgg
     4741 aaatgcgcct agctgcgccc gcccggcgag atgcgatttt ttaataattc gcccccagct
     4801 tgtgccagtc gtcacctgcg aggccgttca ggtcccagac aacctggccg tcgcggattg
     4861 tcagctcgca ctcgatcttt tgtttgcccg cctgccgcgc attgcgtaca tccaggtagc
     4921 cgaactcgcc cgtgcgaagc cgcaacacgg cgacatcggc gttcgcccct ggtgtgaggt
     4981 ggccgagatc gggccgcttg atttcgaggg ccgggttgat ggtgctcatc cgaatcacct
     5041 gctcgaatgg cacgccgaga atcagaattt tcgacatggt ggtagtcata tctttcatcc
     5101 cgccgttcat actccggacg tgcaggtctg tggaaatcga atccggaatc catccctggc
     5161 gaatcgccgg cgcggcctgg cgccacaaaa agcttcccgc gccgtggccg acgtcgaact
     5221 tcaccccgcg ctggcgcgcc tggaaaaggt acggaagaac ctttccctta tcatccagca
     5281 tgggcacagt tcccagatac atgtgcgtgg agatgtcgcc cgcgcgcaat ttttccagca
     5341 ccagttgctg gtagggccgc tccggacgga acgttccaaa gtcgaccatc accggaatgt
     5401 ccgccagttg tcccgccctg accgcccgct cgacggccgt ccactccggc ccttggtaat
     5461 gggcggtctt aactccgaca atgagctgcg gatattgcct cgctacgcgg gctgtggctt
     5521 ccgggtccat gtccgaggtg tcctgctcga ccgccccgca catgcccgtc cccacgatgt
     5581 tcagcatggc gaggatgcgc gttttggagc gatcgatcac ccggtctttg aaatcgggga
     5641 agctcttcca gcccgaggtg ccggcgtcaa ccacggtggt tactccgctg cggaatgtgt
     5701 agccatccgg atagacgctc aggtctccgc aatagacgtc gcgccggccc gtgcctgcgt
     5761 aaacgtgggt atggatatcg accagtcccg gcacgacgta aagcccggct gccgggatga
     5821 cgcgcttggc ttgcgagggg gcgatgttgg gggcgatctg agcgatcttg ccggctgcga
     5881 tcgccacatc catgacgccg ttccggttgt tgcgggggtc gattacatga ccgcccttca
     5941 gcagcagatc gtaaggggcc tgtgcggcgg cgccggccgc cgcggctata aaacaaacta
     6001 ccagccattg tttttgcata aaatgaaacc tcagattgcg tcctttcccc aatgttcagc
     6061 cgcaaagctt gtctgcccga gcgcggccgc cgcgccgcga tcggacatcc gcgcgggttg
     6121 cgctacgaga ccgatattct agcttgttac aatagttgca gcgtcttttt gctcctatga
     6181 ccagcgcgga acaccgccgg ctcgctctgt acgaggctct gcaagcgcgg attttggtgc
     6241 ttgacggggc gatgggcacc atgttgcagg aaaggcgcct cacggcggcc gatttcggcg
     6301 gagcggcgct cgagggctgc aacgaatacc tggtgaaaac ccggcctgat gtcgtactcg
     6361 acatccaccg cgcgtatttg gacgccggct ccgatattat tgagactaac tcgttcggcg
     6421 gaaccagcat tgtcctcggc gagtatgaga tcgcgagcca ggctcgtgat ttgaatttgg
     6481 ccgcagcccg gctcgcccgc cgggcggcgg atgaattttc gacgccggcc aagccgcgct
     6541 tcgtcgccgg ctcgatgggg cccaccacga aagccatcac cgtcacgggc ggcgtgtctt
     6601 ttgagcaact gcgcgaagcc tactacgaac aggccctggc tctggtcgaa gggggagccg
     6661 atctgctgat tctcgagacc tgccaggata cgcgcaacgt gaaggccggc ttgctggcga
     6721 tcgcccgggc ggagcgggaa agcggccgga agatcccggt cattgtttcc ggcaccatcg
     6781 agccgatggg gaccatgttg gcgggccagg ccgccgacgc cttctgggtt tccatctccc
     6841 acgcggactt gctggccgtc gggctgaact gcgccaccgg cccggagttc atgacagacc
     6901 atatccgcac gttgagcgag ctggcctgga cccgcgtctc ttgctatccc aacgccggcc
     6961 tgcccaacga ggaagggaag tacctggaga cgccacaatc cttggccgag cagttggaac
     7021 gcttcattca gcacggatgg atcaacctgg tagggggctg ctgcggaacc actgctgcgc
     7081 acatccgggc catcgcccag atggccgagg gcaggccgcc tcgagtgccg ccgcaacgtt
     7141 cgcggcgggt tttttacagc ggcatcgagc tggtcgaagc ggaagagagc aaccggccgc
     7201 tgctggttgg cgaacggacc aacgtgatcg gttcgcgctt gttcaagaat ctggtggcag
     7261 aggaaaaatg ggaggaagcc actgagatcg cccggaggca ggtgaagggc ggagcgcaga
     7321 ttgtcgacgt ttgcctccaa tcgacggatc gggacgagat caaggatatc ccgccgtttt
     7381 acgaaaagct gatccgcaaa atcaaagcgc cggtaatgat tgacaccacg gacgcgcgcg
     7441 ctgtcgagct ggcgctcacc ttttgccagg gcaagagcat cattaactcg atcaatctgg
     7501 aggacggtga ggaaaaattc caccgcacgt gcccgctcgc ccgcgcctat ggggccgcgc
     7561 tcgtggtggg ctgcattgat gaagacaaat cgcaggccca ggccttcacc cgggaacgga
     7621 agctggccat cgccgagcgc tcctatcggc tgcttacgga aaaatacggc atccagccgg
     7681 aagacatcat tttcgaccct ctggtttttc cgtgcgccac cggcgacgct aactatatcg
     7741 gaggcgcggt cgagacgatc gagggcatcc ggctgatcaa gcagagaatt ccgtttgtga
     7801 agaccgttct cggcatctcg aacatttcgt tcgggctgcc gccggccgcc cgcgaggtgg
     7861 tcaactcggt ctttttgtac tactgcacga aagccggcct ggacctggcc atcgttaatg
     7921 cggagaaact cgaacgcttc gcgtccatcc ccgccgggga acggcaactg gcggagaacc
     7981 tgctcttcaa cacgccgccg gtcggcacgg ccgctcccga agactggcgc ttgcaaacgc
     8041 cggagcagaa agcggctatc aaccagttcc atatcgccgc catcaccgaa cacttccgca
     8101 aggcaggggc gaagacggga gcgaaagcgg cggatctccc gctggacgag cgcttggccc
     8161 gttacatcat tgaaggcacg aaggacggcc tgatcgccga tcttgatcag aagctcaagg
     8221 agggcgccgc tccccttgat atcattaacg gcccgctgat ggacggcatg cgagaggtag
     8281 gagacctatt caataggaat gagctgattg tagcggaagt gttgcagtcg gccgaagcca
     8341 tgaaggcggc ggtcagctat ctcgaacagt tcatggaaaa agctgagacg gctgcgcgcg
     8401 gcaagattgt cctggcaacg gtgaagggcg acgtgcacga tatcggcaag aacctggtcg
     8461 aaatcattct cagcaacaac ggctatcagg tgatcaacct gggcatcaag gtgccgccgc
     8521 aggatctgat tcaagcctat catcaataca atccagacgc catcggtctt tcgggcctgc
     8581 tggtgaagag cgcgcaacag atggtaatta cggcgggcga tttgcgggaa gcgggcgtcc
     8641 gcgtgccgtt gctcgtaggc ggcgcggccc tttccgacaa gttcactcgc cggaaaatcg
     8701 cgcccagcta cggcgaggcg gtcctctatg cgaaagacgc catgaccgga ctcaacctga
     8761 tgaaccggct gatggacccc cgggaacggg aggccgtgct cagggcgcac aagtccgcgg
     8821 aggaggcggc cgtcgggcaa gaggctccag cagcggccat tggcgacggc cagggtgtcc
     8881 cgcggatccc gcagactcgc agccgaaaga tccgggtaga cataccgatc cctccggcgc
     8941 cgtatctgga tcggaaggtc cggcatttgc cccaactggc ggaaatctgg agctacatca
     9001 acccccaggt tttctacggc cgccatttgg gattcaaagg caattttgag aagcgattgg
     9061 cggaacacga cgagaaagcg ctggaacttc atgcgcgggt ggaggaattg aagcaggaag
     9121 cggcgcggtt catgaaggtc cgggccgtgt ggcagttctt cgaggccgag cgggaaggca
     9181 actcgatccg cctgtttggg ccaggcgagc gaaatcccct ttacaccttc cacttcggcc
     9241 gccagccgcg ggaagatggc ctatgcttga gcgattacat cctcgatgcg gccaacgggc
     9301 agcgggacca tttggctttg ttcgtggtga cggcgggcga gggaatccgc gatcggctcg
     9361 aagcggcaaa aaagtccgga gaatatttca aatccatcgc gctgcacgcc ctggcggtcg
     9421 aaaccgccga ggccgccgcc gagtggctgc accggcggat ccgcgaagac tggggcttcc
     9481 ccgacccgcc cggcatgaca atgcaggagc gtttcacttc ccgctatcgc ggcaagcgct
     9541 acagcttcgg ctacggagcc tgcccgaatc tcgacgacca gcagggcatc tggaaactgc
     9601 tgcggcccga agacatcggc gtacatttga ccgagggcat gatgatggaa ccggaagcca
     9661 gtgtaagcgc gctggtcttc caccatcctg attgcgccta tttcaccgcg agcgaagaaa
     9721 ccgagctggt gactgcatag gcagaccttt ttttcgccgg ccccgttccg gccttgcgtt
     9781 tctccggcgc ccccgcatgg caggatgatg cgagaacagt ggtggcaggc gatgtatgga
     9841 gagacctctc cctctcttcc tcaagatcgt atttgcgctc ctccatcttc tggcggattt
     9901 ccttgtttac tggctcctgt tcggcggcgg cttgcgggca gcgcagcggt tcggcctccg
     9961 atgggagccg ggggatttcc cgcggcgcgt gctgctaatg ggctgctcgg cggtgtattt
    10021 tctccgcacg ctggtgacgg cattcgtctt tctcaaacgc aggatgacgt ggtccgaggc
    10081 cgcgactacc gcagtttggc gtctgctggt tcacgctgct tttgccgtgt caggcggaac
    10141 taatcgccgg ccggtagggc tgctagcggt aataggcgcg attctttatt gggccggatc
    10201 ttgtctgaat actggatctg aattccagcg ccacgtctgg aaacaaaagc cggagaacca
    10261 ggggaagctc tacacgcaag gcctgttccg tcttgcgata cacatcaact atgttggcga
    10321 tctcgttctg tttgccggct ttgcaatgat tgcgggcagc ccctacacgg ctctcatccc
    10381 gccgctgacg ctggccgggt tcgtgatctt caatattccc gggcttgaca gacacctctg
    10441 cgaaaaatat ggagccgcct ttgaagagta tgcctgccgg acgaaaaagc tcacgccgtt
    10501 tgtctactga atttctagcc ggtgaactgg ccggacgcct cgccagcgcc gatttttccc
    10561 gcgcggctgt ttttttttgg gcggcgttgc cgtgtggatt caccggccag ccgctgcaag
    10621 tgcaccctaa gaaaagcggc cgcctctgcc cggtacaatt ctgcaaattg caaacgcacc
    10681 ctccagagcg gcggccaaag ggccgcgctg ccgtttttgg gaagcggcgc tctcagaggc
    10741 tggtccctca tatcacccat ccgtactatt tgtactcgca aaaaatgtac ctcgcttcca
    10801 gccgcgagct ctgtgatact tcaggtgttt gaaaaaagaa aggagctgct tcatgcgaga
    10861 cgggcgagcc cttgctatgc gtttcctctg cgagcaagaa gggcaagata ccatggagta
    10921 cacgttgttg ttgggatttg tggctctcgc cgcggcggcg gtttttcccg tgctgagcgc
    10981 caacataaaa gtcatctggc aaatcgccag caaccacctg tcgaattcag ccagcgccgc
    11041 caagtgatca gcgtctccaa gcggagccat tgggaggagc gctgccgggg agcgatagag
    11101 ggagcagcgg ccgcaatcaa tggtctttgg agagtgcatc gccgtttcct gggtcaatgt
    11161 ggcgagggct acgacgggga gagcctgccg gcgtatctga caagaacacc aaagacgccc
    11221 cagtgaaaat gctaaaatcc tgaccgtggt gaacagccgc gtgttgtcgc gctggcggcc
    11281 gagtggaatg ccgtcccact ggttttcagc gcggggcgaa gcatggccgg tcattcggtt
    11341 gcctaaaaga gacagccggc gttgcgaccg atcgccctgt gtttcgggac aggccggcca
    11401 gtgtttttcg gccggcaatg tttgcagcac gcggccggcg cctggcgacc tcggcgtccg
    11461 gcgtcgcctt cccggcgcgt atattcgcgt atattgaggt gaaaggatgc gtaagctcat
    11521 actcgttttg ctggcggcgg cagctgtgtg cccggcccaa tatgacgttc tcatccgcaa
    11581 tggccgcgtg atcgatggta ccgggaaccc ctggcgccgc acgaacatcg cgattaacgg
    11641 cgacacgatc gcggcgatcg gccctttgat cggcgcaacg gccaagcgga ccatcgatgc
    11701 ccgcgaccag atcgtttcgc cgggatttat cgacattcat acgcattcgc gccgcaacat
    11761 tgagcaagtc cccaccgccg acaattacct tcggcagggc gtcaccactc tgatcgaagg
    11821 cccggacggt agttcagaac ttcccctgcg cagcctgttt gccagagtct cagctaaggg
    11881 cacctcagtg aatttcggga gcatggtcgg ccagggaacg gtccggcagg aggtgatggg
    11941 tctggtaaac cgcccggcca caccggcgga gatcgagcgt atgaaggcga ttgtgcggag
    12001 cgagatggaa gcgggcgccc tgggcctctc cacgggcctg ttttatgtac cgggcaactt
    12061 cacccctacg gctgaagtga cggaactggc gcgggtggcc ggcgagatgg gcggcatcta
    12121 catctcccac atgcgggacg aatccgacaa actgatcgag agcgtcaagg agactatcgc
    12181 tatcggcgaa aacgcccata tgcctacgca aatcacgcat cacaaggttt tgggcgcgcc
    12241 ctataagggt gaaagtgtcg agacgctcaa gctggtggaa gaggctcgcg cccgcggcgt
    12301 cgatgtcact ttggaccagt acccgtatac agcttcctcc accggtctca ctggtttggt
    12361 tccacaatgg gcgcaggaag gcgggcacga cgccatggtc aagcgctttc aggatccggc
    12421 aatacggccc cgcatcaaag ccggcatcgt cgagaacatt aaaatccgcc gcggcggcgg
    12481 cgatccgaag aacgtgcaac tggctgtctg ccagtttgat ccgagcctca atgggaagac
    12541 cttggcccaa atcacccagg agcggggcaa agagcctacc gccgagaatt ccgccgacat
    12601 taccatggaa atcctttctc acggcaactg ccaggctgtt tatcacgcga tcgacgaatc
    12661 cgatatcgag cggatcatga aatatccgga tacgatgatc ggctccgacg gcgaggtggc
    12721 cgtgttcggc cggggcgtga tccacccgcg cagctatggg actttcgcgc gtgtgctcgg
    12781 ggtttatgtg cgcgagcgca aagtcatcac cctgcaagat gccatccgga aaatgacttc
    12841 ccttccggct cagcgcatga acctgatgga ccggggcctg ctccggcccg gcatgaaggc
    12901 cgacgttgtt atcttcgatc cgaatgtgat cgcggacaag gcggaatacg gcaacccgca
    12961 tcagtatgca gttggcgtct cttacgtgtt ggtgaacggt caggtggcca tcgatgacgg
    13021 gcggatcacc gccgcgcggc cgggccgcat cctctacggc cccgcccata agaattaaga
    13081 atggtttttt agaagtttcg ccccggatag gggccgtacg aacggcccca tgcaatttgc
    13141 ccggcgggct tagctgattc caagcgagct tccggaaata atgatggcca cgtgccgcat
    13201 ctgcggagcg ccagccgggg ggcgcatcgt caaaccccgc gcggtcatcg aagcggcctg
    13261 gatcccttgc tgaaccaggt agtcgcgaat cccagccgcg cggttcgcca tgagctgctg
    13321 atcggaggtt ccatttgtat acccttcgac ttccaccttc agttccggat gcgcccgcag
    13381 aatggatacg acttttgcga gagcatcgag cgccgggggt tgcaagccgg ccgcattcca
    13441 ctggaaggca gcgtcattca aatcgacgac gagcccgcgc gctgtccagc gggttggccc
    13501 caaactgctg agctgctcga agagttgcgt tcgcaggtcc gcaggggctg cggccggctg
    13561 atcggccgcc tctccggcgc ggatagctct ggcccgctgc tcttccgcct cgcgctcggc
    13621 cgcggttttc tgctgctcgg cgacggctgc ctcggctttg gctcgtgcgg cagcgcggag
    13681 agccgcctct ttctcggcca aaagccgctc ttcttccttc ttgctcgcgg tgatggttct
    13741 tgcgtcctct gcggcttgtg cggcttcgcg ggcggaggtg atcacttgct tgggcgaagc
    13801 cttccggctt tcgtaactct gcgcctgctg taatagctgc tgggcttttt ggaaaacgtc
    13861 gggagcgtag gtgtcggcat gttccgcctg cgcgatctgc acggcgttct gggcctgata
    13921 cagcgccacc acagcctggt agcggtccat ggatagcttc ttgccttcgg gctgcgcctg
    13981 tgaggcgccg gcgttcaacg tgtatccttc gcggcgcggc agcaactgcg cattcgccct
    14041 tacctcttca atcttccctt gcgtatcggg acgcacggcg ttctccagga cgaccacatc
    14101 gctcggctgg gtcaccgagt aatagggctc ggccgtcacc aggagaccga aggcctggaa
    14161 gcgtgttgtc acatgcagct tcgcatttcc gtcggcgtcc ggcactacct cgcccagatt
    14221 tgttgcgcgt ccatccggcg tgatcgccca gagcacgtag ctgagaaact cggggccgaa
    14281 cctggtcgca tccggcaggc gctcaatcct ggcgtcgatc ttcgttgcgc cctttttact
    14341 ttccacaacc gcctcgcctt tggcctgtgg cgccagaacg gttccgcgaa aatcgatttt
    14401 ggtcggctcg ctcaggtgct cgtaattaac cgccttgatg gttcgtccga caaccgtgat
    14461 gcggtaaatg ggcgtccgcc cagcgttttc cggagccgca ttttgcacgg cttggctcca
    14521 ggctggcgcc gatcccaata aaagtgcgag aaaagtcacg ttgacgggct tcatattgga
    14581 cctctccgga cgattcccgc agttgccctg ccactccttt ctccccgcca tttcaatgaa
    14641 atagaagaaa tgctaccgcc cacgggccgg taaaaactac aggtttcctc gggctggccg
    14701 cacgggccct gaagaggctg tgttccgacc gtttcacaaa gtcatgaggg accttgaacg
    14761 gcgtcagcgg tttctagggc cggcctccgg tctcgcccgc gagctgggga gggcgatcag
    14821 aaaagtgagg ggtcgaaggg gactcggatg gtcttctcct cgggccggcc gacaatgaga
    14881 atctcattcc ctgcgatcaa aatatcgcgg actccctcaa agccgtcggg aatcggcgca
    14941 agcaccgcca ggtagcgcgc cgtacgcttt tcttttgcgt tcagccatcc gaaagtcggc
    15001 gtgtcccaaa gcgctggcat ttggaaatag gcgcgcgccg tgccgctcac gcgcgtattg
    15061 ccgaattcca tgccgcgcgt ctgcgtcgcg ccgttccacg gaggctgcat gcgttccttg
    15121 ttctgttccc aaacattcaa ccagggaaaa tcggcgcgac ggaacacata cgcgacgacc
    15181 agccggtatt ctcggttgac ggcggacaga aactcgacat cccgccgcgg atcgagcagc
    15241 aggtaagcca aatccgcgct gcgctgatcc tgcgagaagc gccggaagtc gcggtcgccg
    15301 tccgtcggcc aatgaaactc gtgcgatcgg tcacccaagc tccgctcgcc gcgtgtggca
    15361 gaggcgtcaa atgcactgaa gttcgcgtcc acgaacggcg gaccgaacgt aacgtgctcg
    15421 acccatccaa tggggcggtc gaaagcattc tgattttcag ccgtttcctc gaaatacagc
    15481 accggctggc cctggcgcag gcgcagcgtg cgtgtcatcg acgtgcgccc ggcaggcaaa
    15541 tcggcccgat aagtgaaatc gatattcccg ctggattcgt gcgatgtcac gacataccac
    15601 gccagcgtag atacctcgcc gtgatagctg aggcctgcct gaaactccga tggcgaaggc
    15661 gcgccccaga aatcgaatgc caggttgtgg cccatgatac ctgacagagt tgcggcttcg
    15721 gaatccgttc catacacggc cccgtacttg tctttgttgt aggtagaagg ctcgatggaa
    15781 ggccagggcg gaatccataa cggattgatt tcccggccgc cgggcgggtt tttgagtaca
    15841 agttcggcga tgtggcctcc gccctgaaga acggtcacac gcaggcggtt gttctcaagc
    15901 cgccaagcca ggcgtccgcg aaaacgggcc atgccgggct gttgacccca agctatagcc
    15961 gagacaaaaa ctgccagcag gcaaattttt ttcattacga aacagctctc catcggatca
    16021 agtttccgtc gcgaaccgct gctacctggc cctgccgccg cagatattcg aggtgcgcga
    16081 gcgcctcggc cattgcaaag cgttcattga gcggagacag gcggcgtccc cattcggccg
    16141 ctgcgacctc gtaagcggta cgcggcgttg ccgccaccgc gctgtgcatt cgccggcagc
    16201 gggcctcgtg gtgcagcatg gtagcgcata tccaatcccg gtgatttcgg aaaggctggc
    16261 cgtgcgaagg cagcacccaa tcgatgtcta actcccggac gcgctccaag gaagcgagat
    16321 aatctcccag cggattcccc gaagaatgcc cgtgcaagcc tatattcggc gtgatcttct
    16381 ccaccatgtg gtcgctcgaa aagtacagtc ttcggccggc gaagtagaag gtcagtaaac
    16441 caggggaatg gccgggagtc aacagagtct cgatctcacc cccgtgaata ggaatccggt
    16501 cgccatcttc cagaaagcct tcaggcgaaa acatatcgat gaactcggag atgcctcgcg
    16561 aagcctgagc gatctcctcg gtctcgtccc ggggcgcccc gttctcggtg agccaagccg
    16621 actgctcgga gaacaggtca ttatccataa atctcgggga gagcagggcg gcctccgctg
    16681 cgtgcaccca gatgccggcc ccggagcggt tttgcacttc ggcggccagg ccgaagtgat
    16741 cgggatgcag gtgggtgata atcagccgcc ggatatccgt ccacggcacg ccgatggacg
    16801 agagagcctc ggccagagct ttgcggcagg cgttcgtctt caagccgcaa tcgagcagaa
    16861 gaaaaccgtc ccctgtacgt atcaagtaga cattcaaaat agcgacggga aacggcagag
    16921 gcagccggat cagccagatg ccttcagcaa tcgagactgg agcagtcata aatcatggcc
    16981 gagctgagac gcccgccgac gattcgggcc aaaggcagcg gagcacgcgc ctgggccggg
    17041 aagtaagcct atttcaagcc tccggcatcc aaaggctgct cggcgagcgc cgcctctccg
    17101 gccctggatc tcgccagcgc ccaagtcgag cccgcggcca cggagacgaa gagaagacct
    17161 tctccccatt catgcagcaa taattttcca atgccgaaca gcgagctgta gatcaaaaca
    17221 cagcctgcga accagttccg aaaactcccc gcgaggtcgt ccgccgccgg aacgccggct
    17281 ttcgcggcaa ccgggcccca gagcacaggg gagggccggg cccggcggta aaacgccacc
    17341 agggtttcct ccggctccgg cttcgtcaaa agcgttacgg ccacccaaac cagggtggta
    17401 gccgccacgg tgacgagcat cagtttggcg aagccaatgg ggtcgtcgct atcccacccc
    17461 acgcccaatt gcaaatacaa agagatggca aacgccg
//
LOCUS       DASREK010000205        10977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2404700, whole genome shotgun sequence.
ACCESSION   DASREK010000205 DASREK010000000
VERSION     DASREK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10977
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404700"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..196)
                     /locus_tag="VFA54_10610"
     tRNA            complement(120..196)
                     /locus_tag="VFA54_10610"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Val,seq:cac)
     gene            complement(597..1247)
                     /locus_tag="VFA54_10615"
     CDS             complement(597..1247)
                     /locus_tag="VFA54_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51304.1"
                     /translation="MPHPRSKGSTGYPDALKETAAFRDENYRGVGFRWGMGLDGSEVR
                     LCDYRCMPLFDDMNNWVADLPTPPKYMQAISEVYQQGPGDDVYANNPVNYVKVDALPG
                     IDNWKPIVDSMKRGAYFVTSGEVLIPSWSMQGSGNRRTIVADVEWTFPLEFAEVIWGD
                     GQRTGRQIIPATDLPALGKHHFQIPFNANGEKWVRFAVWDSAGNGAFVQPVKLAGQ"
     gene            complement(1847..3016)
                     /locus_tag="VFA54_10620"
     CDS             complement(1847..3016)
                     /locus_tag="VFA54_10620"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS51305.1"
                     /translation="MLLALFCLFSAAAIALGVASLHSGQRQWNAIRSQVQAGPPLWTP
                     PATLIVPIKGLEPGLVDNLRSLLEQDYPDYELLIAVRDSADPAVAQIKPWLNSRARLV
                     VAGRGPRDTSEKIGNLLAAVSQARANSEVFAFADSDGRAGRGWLRGLISPLRDPAVGA
                     ATGYRWYFPDRGEFWPLVQSVWNAAIAGTFGAGDPPFAWGGAMAIRRNIFEKARVAEY
                     WLGSVSDDYQLTRAIRAAGLRIAYSAHAMVETGGGCSACEFLEWAVRQLTIARIYDAR
                     TWWLGLAAHAVYCAAMATGIAMLAEGRFWAAPVFFLTFVPGMCRGAWRERVARLLFPA
                     RKPWFDHHGWVYCWLTPAVTWIWLWVFLASLFRRRIEWRGNVYELLGPSQTKVEE"
     gene            3087..4505
                     /locus_tag="VFA54_10625"
     CDS             3087..4505
                     /locus_tag="VFA54_10625"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZS51306.1"
                     /translation="MFGRHKKKVVFLFALADVILTIFSFEAAYQLRVYLPLEHVFFMT
                     VPVKALVLGAALAAWVSMGFLFRVYERLETGEPIAALGDSIQQVTASLLLLVAFLFGM
                     KLDISRVFIGLFGAFNLLALVVYRVSARSVRGYLRKRFGAVSYFVIVGTGKASREVGR
                     ALEQAEQHGVRLLAYIDPAEQPCGAQIALEKTYPVYPLSELPALLRSQVIDEIIFAVG
                     GERLPKLEEIFLLCDEEGVRTRVLLDFFPHVNSEVYLDQFGSFSLLTFSATPHDEIRL
                     LIKRVFDFVFALIALIVLALPLAVFALAVRLTSRGPAIFKQVRCGLNGRRFTFYKLRS
                     MCHDAEIKKAELAALNERDGPAFKISNDPRLTPLGRYLRRFSIDEWPQLWNVLKGDMS
                     LVGPRPAVPAEVEQYKGWQRRRLRMKPGLTCLWVLEGRDHLDFETWMRLDMKYIDNWS
                     LLLDLKILLRSFPHILLGRGAS"
     gene            4515..5237
                     /locus_tag="VFA54_10630"
     CDS             4515..5237
                     /locus_tag="VFA54_10630"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZS51307.1"
                     /translation="MIPLRDNIPSRRYPVVNVTLIILNVVVFLYQLMLGPDLESFVRT
                     YAVVPYRYFHAVYVTSHGLRPVTTADLLIPLVTSMFLHGGWLHLIGNMWYLWIFGDNV
                     EDRLGHGRYLIFYLLCGITAALAHIWFNAGSRLPSLGASGAIAGVLSAYLISFPRARV
                     LVLVPLFFFFPIVEIPALFFLGFWFLQQFFYGAASLGVPAYQTGGVAWWAHVGGFVSG
                     AILLWAFAPKGRYRPASYSVWG"
     gene            complement(5219..5926)
                     /locus_tag="VFA54_10635"
     CDS             complement(5219..5926)
                     /locus_tag="VFA54_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51308.1"
                     /translation="MIRRGILLGLLLCAVFMGPARAQSRHTLSVSGTSFLLNGAPFPY
                     TGISFFNAVYNPSFNKRSEERRQWEKFQRYGINSLRVWAQWDTRRSYADTCGACTLYF
                     PDGRLRDRNVARLKEIIADADRLGMVIELVLFSQESWHANIRLGPEESHRAVTALTRE
                     LLPYRNMTFEIWNEFSERVLDHLKTIEAIDPGRLATNSPGGAGILGDREQNEALDYLS
                     PYPTRQSGDRCFQPQTL"
     gene            complement(5932..6825)
                     /gene="proC"
                     /locus_tag="VFA54_10640"
     CDS             complement(5932..6825)
                     /gene="proC"
                     /locus_tag="VFA54_10640"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZS51309.1"
                     /translation="MPVSQSSASRRTGAKTTRSRRIAVLGAGNIGAALIGGILKGKVA
                     RREDLIATVRTPERAEELAAKFSIKVTAGGNREAAQNSGLIILAVKPSTLPKVLEEIR
                     DVLREDQILISLAAAVPISLIEKLIEKRMPIFRAMPNIPVVVEEGATAVASNAAGTAA
                     HRRLVEDIFRAVGVVTFVEEEMMHAVTALSGSGPAYVYMVIEALIAGGLKMGLSREVS
                     TRLAEQTVLGAAKLVRETMLHPAILKDQVITPGGVTISAIHELEKHGLRSMLISAIET
                     ATHHSQNLTRSLLAKFGKEGQ"
     gene            6925..9366
                     /locus_tag="VFA54_10645"
     CDS             6925..9366
                     /locus_tag="VFA54_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZS51310.1"
                     /translation="MAGRLTILAALLVSSLLRADTGAERLRKIRAISFSPEQCYRVRD
                     LLLEREDFKLYFTDGYLLFANPVEGQSLAALFLAATDTGEAEIVVMPPSPSERQSLAR
                     FAGAPVLEENIRTALMFFTDDTAQVLRRAMAENEFNHPDVEAGKRLAPDWQAVTRNMI
                     TNYDARMLMDAFTQPEASGVPRTGFFAAAISSVKLGRFDILLDPLRTEQAILGQVVWQ
                     AGQRYYDIWASFPERSLRSRGAERAQFLIDPGALEHYRIEAWLSADLSMNVDARATLI
                     TSGVRQRAVLFEISAQMKVTQVQLDGRPVEFLQGGALDSSSSRRRGDDWVLVISEEPL
                     EPGSHHQIEFRYEGNVIADAGGGVYFVGDRGNWYPSRGLHFTDFDLWFHYPSRLQMVA
                     TGRELESQLEGDVRTSHWRPDAPIRVAGFNLGNFERASLKLGSYTLDVCANKTLERAL
                     QPMPVVAPPPLPPPRRRSPFRNPSIAPDLPPPEAPPSPTLRLEQVAQDSAEALQFFTQ
                     HFGPLPLRHLTISPIPGRFGQGFPGLIYLSTLSYYQAEDKPLEKLRAEDRLFYAEQLR
                     AHEIAHQWWGNVVTTLDYHDEWLMESLADYSALMFLEQHKGPDVLEKVLGVYKSNLLS
                     KTESGQTVESAGAIVLGNRLRSSRSPRARETITYEKGAWVLHMLRRRMGDRNFLGMLT
                     ELRRKYEYKSVNTEQFRELAAKFLPPNSPDPSLESFFSQWVYSTGVPVLQMEYKVTGK
                     APQFRVAGVIRQSGVPEDFSVAAPVEIQVPGGGRKIETRVQTANGETPFSITLRERPA
                     RVQLDPQDSILAIKQ"
     gene            complement(9470..10327)
                     /gene="thiD"
                     /locus_tag="VFA54_10650"
     CDS             complement(9470..10327)
                     /gene="thiD"
                     /locus_tag="VFA54_10650"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZS51311.1"
                     /translation="MAQVSSRPVALTVAGFDPSGGAGVLADLKTFAAHECYGVAAIAA
                     MTAQNTREVRKVMPVDPEMLRLQLDMLFEDISIAGIKVGLLGGRKNLEIVAACLSAHK
                     ELPIVVDPVLRSTSGTQFLPPQDIEPFKRLLFPLVHVLTPNAEEAGRLLGASVSTLEE
                     MKGAAKLLHEQGVRHVVITGGHLDKPTDVYYGGQEMEVFTGHRIESQSTHGTGCTFSS
                     AILTNLIHGKAAMESVVLAKAYVTQAINRAYPIGNGRGPLNHLFRFGEPAAPVRVGGP
                     EPAHDSSQQ"
     gene            complement(10617..>10977)
                     /locus_tag="VFA54_10655"
     CDS             complement(10617..>10977)
                     /locus_tag="VFA54_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZS51312.1"
                     /translation="APDVIHQLRESAVPGGMAHACELETSVYLHLDSARVQMDKAVKE
                     MNLPASKFIWLDLMKGSPVLYMDHWTRFSRSGVVGDPTLATAEKGKKIFEAVVAALVE
                     LVREFKSRSRGERADLH"
BASE COUNT         2106 a         3349 c         3357 g         2165 t
ORIGIN      
        1 ggttccgggc tgcctcaagg gttcatccaa tctacgcagt acgggctggg ccgcagcaaa
       61 tacgggcgca agtgtcagcc gcttttcacc tgacgccact gcacaatcgg tggagaagat
      121 ggtgggcgcg acaggactcg aacctgtgac ctcctgcgtg tgaagcaggc gctctaacca
      181 gctgagctac gcgcccggat caacgaaatc ggagcctctg aggcagcggc cgcgatcgga
      241 gcaaaagtct gcggaagccg cccgaaagcc attcagatgg aaagccggat cgggcttgac
      301 cgaacgcgcg cccgcgccac ctggcgttcc gggtccgcgt tccattgacc attccagctc
      361 cgattcaatc cctctaagca taccgcgaaa tccgagccgg cgtcccacgc gtgaaacggg
      421 cgcgtccggc ggcaaactct aagtcaggca aacagcaatg aagacttacg cactcgccta
      481 gtcgacctca cggcgcccag gccgttaaga tacgcgaggt tcgggctcgc gtggggctgc
      541 cgcgcacgtg ggcggtgggg ccagcaattc tgtcaagccg cgccggtacg tggcactcat
      601 tgccctgcca gcttcaccgg ctgcacgaac gcaccgttgc ctgccgaatc ccaaacagca
      661 aagcgcaccc acttttcccc attggcgtta aaaggaatct ggaagtggtg cttacccaac
      721 gccggcaaat ccgtggcggg aatgatctgc cggccggtgc gttggccgtc gccccaaatc
      781 acctcagcga actctagcgg aaaagtccat tcgacatccg ccactatggt ccgccgattt
      841 cccgatcctt gcatggacca ggaagggatc agcacttccc cggatgtcac gaagtaagca
      901 ccacgtttca tggagtcgac gatcggtttc cagttgtcaa tacccggaag cgcgtcgacc
      961 tttacgtaat tcacagggtt gtttgcgtag acatcatcac cgggaccttg ctggtaaact
     1021 tccgaaatcg cctgcatata tttcggtggt gtaggcaggt cggcgaccca attgttcatg
     1081 tcgtcgaaca aaggcatgca gcggtaatcg catagccgaa cttccgaacc gtccagtccc
     1141 ataccccagc gaaaacccac cccgcggtag ttctcgtcgc ggaaggcggc tgtttcttta
     1201 agggcatcgg gatatccggt ggaccccttc gaacgcggat gcggcatgaa aaggatcagg
     1261 gccctggacg gtacactgac cacctcctag gccgaaagtt ccatattgaa acccagggtc
     1321 aaatactctc tatacccgtc cggtttcgtt gtcaagtggc actgtccgaa attgctttcc
     1381 ccgacggggg acagcctttg ttgcgcaatg ggggcctatt ccgggaggcc gagcgcgagg
     1441 atggcatgga ctattcgggg gtgaagtccc cggccggttt caggcgccca tcgccgtttt
     1501 tgctgttatg tgggcgattt cgaaacccag gcgctccgca ttgtgcagtg aggccgtccc
     1561 gggccgggta atgctaacag ataggttgca acctgaggaa actgcgtatt cgatgcgggc
     1621 gcgcgagagc gccgcctgat accgcggcat gcaacgatca cagccgcttg tgcgcctgct
     1681 cttcgattcc gcgaaacttc cggctgccga atccttcatg gagggccttg gtttcctggc
     1741 agcggaacta cagaatcatg aacggcaaga gtaggatgcc gcgggtagag agtcaaggtg
     1801 catccgttcc tggagcagca agagactgca attgctgaaa agcatctcat tcctccacct
     1861 tggtttgcga cggacccagt aactcgtaaa cgttgccgcg ccattcgatg cgccggcgga
     1921 agagcgaagc cagaaaaacc caaagccaga tccaggtaac ggccggagtg agccagcaat
     1981 agacccagcc gtggtggtcg aaccacggct ttcgggccgg aaacagcaag cgggccacac
     2041 gttcccgcca ggcgccgcgg cacatgccgg gaacgaaggt cagaaagaaa accggggcgg
     2101 cccagaaacg cccttccgcg agcatcgcga tgcccgtggc catggcggca caataaacag
     2161 cgtgcgcggc gaggcccagc caccatgtcc tggcatcgta aatccgggca attgtcaact
     2221 gccggacagc ccactcgagg aactcacacg ccgagcagcc gccgccggtc tccaccatgg
     2281 cgtgagcgga gtacgcaatg cggagcccgg cggcgcggat ggcgcgggtc agttgatagt
     2341 catcgctgac cgagcccagc caatactccg ccacccgggc tttttcaaaa atgttgcggc
     2401 gaatggccat cgcgccgccc caggcgaagg gtggatcgcc ggcgccgaag gtccctgcga
     2461 ttgctgcgtt ccaaacgctt tgtacgagag gccaaaattc gccgcgatcg gggaagtacc
     2521 agcggtagcc ggtagcggct ccaacggcgg ggtcgcggag cggagaaatc aagccgcgca
     2581 gccagccgcg gccagcgcgc ccgtcggagt cggcgaaggc gaaaacctcg ctgttcgccc
     2641 gggcctggga gacggcggcc agcaggttac cgattttctc actggtatcg cgcgggcccc
     2701 ggcccgccac caccagcctg gcccgcgaat tcagccaggg tttgatctgg gcgaccgccg
     2761 gatcggcgga atcgcgcaca gcgatcagaa gttcgtaatc cgggtagtcc tgctccagca
     2821 gcgagcgcag gttgtccacc agcccgggct ccaggccctt gatcggaacg atgagggtgg
     2881 ccggcggcgt ccataacggc ggcccggcct gcacttgcga ccgtatcgcg ttccattgac
     2941 gctgcccgga gtgcagggaa gcaacgccca aagcgatggc ggcggccgaa aaaagacaaa
     3001 acagggcgag cagcacgcgt tgagtctaac attcttccga tagcccctcg gttccgtttt
     3061 ggtattctgt ccaaaggtaa gtacctttgt ttggccgaca caagaagaag gttgtatttc
     3121 tctttgcgct ggccgatgta atcctcacga tcttttcctt cgaggcggcc taccagctcc
     3181 gcgtgtacct gccgctcgaa catgtcttct tcatgacggt cccggtcaag gcgctggtgc
     3241 tgggcgctgc gctggcggct tgggtaagca tgggctttct cttccgggtc tacgaacggc
     3301 tggaaaccgg cgagcccatt gccgctctgg gcgactcgat ccagcaggtc acggcgagcc
     3361 tgcttctcct ggtcgcattc ctgttcggga tgaagctcga tatcagccgt gtttttatcg
     3421 ggctattcgg cgcgttcaac cttctggcgc tggtagttta tcgtgtcagc gcacgttccg
     3481 tgcggggcta cctgcgcaag cgcttcgggg cggtctcgta tttcgtgatc gtgggcacgg
     3541 gcaaggcgtc ccgggaagtc ggccgcgctc tcgaacaggc ggagcagcac ggcgtgcggc
     3601 tcctggccta catcgatccg gcggagcagc cgtgcggggc ccagatcgct ctcgagaaga
     3661 cgtatccggt gtacccgctc tcggaactgc cggctctcct gcgcagccag gtaatcgatg
     3721 agatcatctt cgccgtcggc ggagagaggc ttcccaagct ggaagaaatt ttcctgctct
     3781 gcgacgaaga gggagtgcga acccgggtgc tgctcgattt cttcccgcac gtcaacagcg
     3841 aagtctacct ggaccagttc ggctcgttct cgctgctgac cttttcggcg actcctcatg
     3901 acgaaatccg cctgctgatc aaacgtgtat ttgacttcgt ttttgccctg atagccctga
     3961 tcgtgcttgc cttgcctctc gccgttttcg ccctcgccgt ccgcctcacc tcgcgcggcc
     4021 cggccatttt caagcaggtg cgctgcggcc tcaacggccg gcggttcacc ttctacaagc
     4081 tccgctcgat gtgccatgac gccgagatca agaaagccga actggccgcc ttgaacgagc
     4141 gtgacggtcc ggccttcaag atttccaacg acccgcgcct cacgccactg ggccggtatt
     4201 tgcggcgatt ctcgattgac gagtggccgc aattgtggaa tgtgctgaaa ggagacatgt
     4261 cactggtagg gccgcgcccc gcggtgcccg cagaagtgga acaatataag ggctggcagc
     4321 ggcggcggct gcggatgaag ccggggctga cgtgcctgtg ggtgctcgaa ggccgggatc
     4381 atctcgattt cgagacgtgg atgcgtcttg acatgaaata catcgataac tggagtctgc
     4441 tgctggatct gaaaatcctg ctccgctcat ttccgcacat cctgctgggg aggggagcgt
     4501 cttaagacga ccgcatgatt cccctgcgcg acaacatccc ttcgcggcgc tatccagtcg
     4561 taaacgtaac gctgatcatc ctgaatgtgg tggtcttttt gtatcaactc atgctcggcc
     4621 cggatctgga aagtttcgtg cgcacgtatg ccgtggttcc ctaccgctat tttcacgccg
     4681 tctatgtgac gtcgcacggg cttcgcccgg tgacgacagc cgacttgctc ataccgcttg
     4741 tcacctcgat gtttctgcac gggggctggc tgcatctgat tggaaacatg tggtatctgt
     4801 ggattttcgg cgacaacgtc gaagaccgcc tgggacacgg ccgttacctg attttttatc
     4861 tgctctgcgg catcaccgcc gccctggcgc acatctggtt caacgcgggc tcgcggctgc
     4921 ccagtttggg agcgagcgga gcgatcgccg gcgtgctgag cgcgtatctg atctcgtttc
     4981 cgcgggcccg agtgctggtg ttggtgccgc tgttcttctt ctttcccatt gtggaaatcc
     5041 cggctttgtt ctttctaggt ttttggtttt tgcagcagtt cttttatggc gcggccagcc
     5101 tgggcgtccc tgcatatcag accggcggcg tagcctggtg ggcccacgtc ggcggattcg
     5161 tcagcggggc gattctgcta tgggcgttcg cgcctaaggg cagataccgg ccagcatcct
     5221 acagcgtctg gggctgaaag cagcggtctc cgctttggcg ggtggggtag gggcttaaat
     5281 agtcgagcgc ctcgttttgc tcgcgatcgc cgagaatgcc cgccccgccg ggagagttgg
     5341 ttgccagtcg tcctgggtcg atggcttcaa tggttttcag gtgatcgaga acccgctcgg
     5401 aaaactcgtt ccaaatttca aacgtcatat tgcggtaggg cagcagctcg cgggtcaggg
     5461 cggtaacggc gcgatgggat tcctcaggtc ccagccggat attcgcatgc cagctttcct
     5521 gtgagaatag cacgagttcg atcaccattc cgaggcggtc tgcgtcggca atgatctcct
     5581 tcagcctagc cacgttccga tcgcgcagcc gcccgtcagg gaaatagagc gtgcaggcgc
     5641 cgcacgtatc tgcgtagctg cgccgggtgt cccactgcgc ccagacgcgc agggagttga
     5701 tgccgtagcg ctggaatttt tcccattgcc ggcgttcctc ggatctctta ttgaacgagg
     5761 gattatagac ggcgttgaag aagctgattc ccgtgtaagg gaacggagcg ccgttgagca
     5821 aaaacgatgt gcccgagacg ctcagcgtat gccgggattg cgcccgcgca gggcccatga
     5881 aaacggcgca aagaagcaat ccgagcagaa ttccgcggcg aatcatagga tctattgtcc
     5941 ttccttcccg aatttggcca gcagcgaccg cgtcagattt tgcgagtggt gggtggcggt
     6001 ctcgatcgcc gaaatcagca tcgagcgcag cccgtgtttt tcgagctcgt ggatcgctga
     6061 aatcgtcact ccgcccggag tgatgacctg atctttcaga atggctggat ggagcatggt
     6121 ctcgcgcacc agcttggcgg ctcccagcac ggtttgctct gccagtctgg tggaaacttc
     6181 gcgcgacagc cccatcttga ggcctccggc gatgagcgcc tcgataacca tgtaaacgta
     6241 agccgggccg ctgcccgaca gagcggtgac cgcgtgcatc atttcctctt ccacaaacgt
     6301 tacgacgccc actgcgcgaa aaatatcttc caccagccgg cggtgggcgg cggttccggc
     6361 ggcattggac gcgacagcgg tagcgccctc ttcgactacc accggaatgt tcggcattgc
     6421 ccggaagatc ggcattcgct tttcgatcaa tttttcgatg aggctgatgg gtacggcggc
     6481 ggcgagggaa atgagaatct ggtcttcgcg cagcacgtcc cggatctctt ccagcacttt
     6541 cggcagcgtg gagggcttca ccgcaaggat aatcaggccg gagttttggg cggcctcgcg
     6601 gttgccgccg gcggtgacct tgatcgaaaa cttcgccgcc agttcttccg cccgctcggg
     6661 tgttctcacc gtggcgatca aatcctcccg gcgggcgacc ttccctttca gaatgccccc
     6721 gatcaacgct gccccaatat ttccggcccc cagcacggca atgcgcctgg accgggttgt
     6781 cttcgctccg gtccttcgag aagcagagga ttgggaaacg ggcatgggga cattttaggg
     6841 cataaagcgc agcgaaacag ccgcgaaagg ccggcgttgc ggaggaagga ctgggggcca
     6901 gggcaaactg atatcatcaa gagcatggcg gggagactga cgatcctggc ggcgttgctg
     6961 gtatcctcgt tgctccgggc ggacaccggg gccgagcgcc tccgcaagat tcgcgccatc
     7021 agtttctcgc ccgagcagtg ctatcgcgtt cgcgacctgc tgctcgagcg agaggatttc
     7081 aagctctact tcaccgatgg atatcttctg ttcgccaacc ctgtggaagg ccaatccctt
     7141 gcggccctgt ttctggctgc cacagacacg ggagaagcgg agatcgtggt catgccgccg
     7201 tcgcccagcg agcgccagtc gctggcgcgg ttcgccggcg cgccggtgct cgaggaaaac
     7261 atccgcaccg cgttgatgtt tttcaccgac gataccgctc aagtcctgcg gcgcgccatg
     7321 gccgaaaacg agttcaacca cccggatgta gaagccggta agaggctggc cccggactgg
     7381 caggctgtaa cgcgcaacat gatcaccaat tacgacgcgc gcatgttgat ggacgcattc
     7441 acgcagcccg aggcgtcggg cgttccccgc accgggttct ttgcggcggc catcagcagc
     7501 gtcaagctgg gccggttcga cattctgctc gatccgctcc ggaccgaaca ggccatcctg
     7561 ggccaggtgg tctggcaggc ggggcagaga tactacgaca tctgggccag ctttccggag
     7621 cggagcctcc ggtcgcgcgg cgccgaaaga gcgcagttcc tcatcgaccc aggcgcgctg
     7681 gagcactacc gcatcgaagc atggctatcg gcggatttga gcatgaacgt ggacgcccgc
     7741 gcaacgctca tcacaagcgg cgtgcgccag cgggccgtgc tgtttgaaat ttcggcccag
     7801 atgaaggtca cacaggtgca gctcgacggg cgcccggtgg agtttttgca aggcggggct
     7861 cttgactcga gctcatcccg ccggcgcggg gacgattggg tattggtaat cagcgaagag
     7921 ccgctcgagc ctggcagcca tcatcaaatc gagttccgct atgaaggcaa cgtcatcgcg
     7981 gatgcaggcg gcggggtgta tttcgttggg gaccgcggaa actggtaccc gagccgcggc
     8041 ctgcatttta cggacttcga cctctggttc cactatccca gccgcctgca aatggtagcg
     8101 accggccggg agttggaatc ccagctggaa ggcgatgtgc gaacgtcgca ttggcggccc
     8161 gatgcgccga ttcgcgtcgc gggattcaat ctcgggaatt ttgaacgagc cagcctgaaa
     8221 ctgggaagtt atacgttgga cgtctgcgcc aataagacac tggagcgcgc cttgcagccc
     8281 atgccggtgg ttgctccgcc gccgctgcct ccgccgcgcc gccggtcgcc gtttcgaaac
     8341 ccgtcgatag cgcccgacct tccgcccccg gaagccccgc ccagtccaac cctccgcttg
     8401 gagcaagtgg cgcaggacag cgcggaagca ttgcagtttt tcacccagca tttcggaccc
     8461 ttgccgctgc ggcacctgac aatctcccct atccccggcc gctttggtca gggttttccc
     8521 gggctgatat atctctccac gctgagctat taccaggcgg aagataaacc cctagaaaag
     8581 cttcgcgccg aagaccgcct gttttacgca gagcagcttc gggctcacga gatcgcgcac
     8641 cagtggtggg gaaacgtcgt cactacgctc gactatcatg acgagtggct gatggaatcg
     8701 ctcgccgatt attcggcgct gatgtttctc gagcaacaca aggggccgga tgtgctcgaa
     8761 aaggtgctcg gcgtgtataa gagcaacctg ctcagcaaga ctgagtcggg ccagacggtg
     8821 gaaagcgccg gagcgatcgt cctcggcaac cgcctgcggt cgtcacgctc gccacgggcc
     8881 cgggaaacga ttacatacga aaaaggcgct tgggtgctgc acatgctccg ccgccggatg
     8941 ggagaccgga acttcctcgg catgctgacg gagctccgcc gcaaatatga atataagagc
     9001 gtaaacacag aacaatttcg tgaactggcg gccaaattcc tgccgcccaa ctcgccggat
     9061 cccagcctgg aaagcttttt ttcgcagtgg gtctactcca ccggcgtgcc cgttttgcaa
     9121 atggaataca aggtgacggg caaagcgccg cagtttcgag tggccggcgt gatccggcaa
     9181 tcgggcgtgc ccgaggattt cagcgtggcc gcgccggtgg aaattcaagt tccgggcggg
     9241 ggccggaaaa tcgagacgcg cgttcaaacc gcgaacgggg aaactccgtt cagcatcacc
     9301 ttgcgggagc gcccggcgcg cgtgcagctc gacccgcagg attccattct ggcgataaaa
     9361 caatagaaac aggcaaacaa cgcgcgggaa ctggtcgcga gcgcctgccc gcaccccgcg
     9421 ccccaggcca taggacgcag gcgcttctcc aagccgaccc tgagggcgct tattgctgcg
     9481 agctatcgtg cgccggctcc ggtcccccga cgcgcaccgg cgccgcgggc tcgccgaagc
     9541 ggaaaaggtg gttgagcgga ccccgcccgt tgccgatagg ataggcgcgg ttgatggcct
     9601 gggtcacgta agccttagcc agaacgacgc tctccatcgc cgccttgcca tgaatcaagt
     9661 tagtcagaat ggccgaggag aacgtgcacc cggtgccatg cgtgctctgg gactcgatgc
     9721 ggtggccggt gaaaacctcc atctcctggc ccccgtagta aacgtctgtc ggtttatcca
     9781 gatggccgcc ggtgatcacc acatgccgca ctccctgctc gtgcagcagc ttggccgctc
     9841 ctttcatttc ttccagggtg gagacagaag cgccgagcag gcgtccggct tcttcggcgt
     9901 tgggcgtcag aacatgaacc agagggaaca gcaagcgctt gaagggttcg atgtcctgag
     9961 gcggcaggaa ttgtgttccg ctcgtggatc gtaaaaccgg atccactacg atcggcagct
    10021 ctttgtgtgc gctcaagcag gcggcgacga tttccagatt tttcctgccg ccgaggaggc
    10081 ccactttgat cccggcgatg ctgatatcct caaacagcat gtccagttgc aggcgcagca
    10141 tctcgggatc caccggcatt accttgcgca cctctcttgt gttctgcgcg gtcatggcgg
    10201 cgatggcggc cacgccgtag cattcgtgcg cggcgaaagt cttcaaatcg gcgagaacgc
    10261 ccgctccgcc ggagggatcg aagccggcta ccgtcaacgc gacgggcctg gaactaacct
    10321 gcgccatttc tactcacctg agttgcttcc gagtgaaaca taacacagca gtttgccggc
    10381 tacaataggc ctgagaaaac aaatcgaata cggcaacggg gggccagccg gccatgcatt
    10441 ctccctccca ccgccggatg cggcggcatg acttgttccg gtgcaatccg gtctaggaga
    10501 cgagaccgct cgggccgagg ttgctacgat ttcacaacgg gtctgatttt ctcctccggg
    10561 cttggccgcc gtgcgggcat cggggttgca accggcccgc caagtccgcc gccggctcag
    10621 tgcagatcgg cgcgctcgcc gcgcgaacgc gacttgaact cccgcaccag ttctaccagc
    10681 gccgcgacga cggcttcgaa gatcttcttg cctttctcgg ccgttgccag ggtcgggtct
    10741 ccgaccactc cgctgcggct gaagcgggtc caatggtcca tgtaaagcac cggtgacccc
    10801 ttcatcaggt cgagccagat gaatttcgat gcgggcaggt tcatctcctt gactgcttta
    10861 tccatttgga cgcgggcgga atcgaggtgc aaatagaccg aggtttcaag ctcacaagcg
    10921 tgcgccatgc cgcccggaac ggcggattcg cgcaactggt gaatcacgtc gggagcc
//
LOCUS       DASREK010000206         6231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1747378, whole genome shotgun sequence.
ACCESSION   DASREK010000206 DASREK010000000
VERSION     DASREK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747378"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..815
                     /locus_tag="VFA54_10660"
     CDS             12..815
                     /locus_tag="VFA54_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZS51313.1"
                     /translation="MSLVRPGDLVSAEMHVACGRCYQCRTGASHICRRVKIIGVDADG
                     CFAEFVRVPESNVWKVDPAITPDIAAVFDPLGNAVHAVLAGPVAAAGIAVIGCGPIGQ
                     FAVAVARACGASRIFAIEINERRLAVACRMGADVLINPAREDPVARVLEETAGSGADV
                     VLEMSGHPQGVKNAFAMLRMGGQVTLLGIPIKPVEMDLAGDIIFRGATVAGINGRRVF
                     QTWYQMEALLKGGRLDITPAITDRLPLAEFHKAMDLLRRGEGSKILLEP"
     gene            886..2079
                     /locus_tag="VFA54_10665"
     CDS             886..2079
                     /locus_tag="VFA54_10665"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZS51314.1"
                     /translation="MNHAMTNPLQYLTDELNELRNKGLYQRLRILGGEQLPVAEFDGR
                     RVINLSSNNYLGLTTHPKLREAAIEATKKYGVGSGAVRTIAGTMSIHMALEEKIAAFK
                     HAEACVVFQSGFAANAGTVAAVLGKDDVIISDELNHASIIDGCRLSRAAIRIFPHKDV
                     AAAEKILAEAAQLPGRKLLITDGVFSMEGDIAPLPALVELAERYGAIMMIDDAHASGV
                     LGKNGRGTVDHFGLHGRVHIQVGTLSKAIGVLGGYVCGSRDLIEFLYHRARPFLFSTS
                     HPPAVAASCLAAFEVLEQEPELVQRLWENTRFFKKGLMQLGFNTGRSETPITPVIVGE
                     ATLAHQFSRELFEAGVFAQGIGFPTVPQGQARIRTIVTATHSRAELDQALATLEKVGK
                     KLKII"
     gene            2307..3254
                     /locus_tag="VFA54_10670"
     CDS             2307..3254
                     /locus_tag="VFA54_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZS51315.1"
                     /translation="MNRREFAASLAAALIAIRSGSEAAEKNISWALSLALWGHLAPVP
                     FTEILDIMKDTGFTGIRMTSFPGCLKQYNLTIPSLQRELDKRGLRIATISFGGPADDR
                     TKHEQIEKSAREALGFLKEFGATELVVFSPARVPKVLVPERIHIAAEFYNHLGDVAKE
                     YGFRAGLHNHLDQLAECQDEVEMFLKLTDPKRFGFAPDTAHLHLSGCNVAELFEKYAD
                     RLIFMDYKDSRNTPAAKDIVLPNGQTLKAGTASATFMNSIYDLGDGEVNFPRLHRILK
                     RRKYKGWICVDLDYVRASARESFGRCMKYVREKLEPIYV"
     gene            3556..4203
                     /locus_tag="VFA54_10675"
     CDS             3556..4203
                     /locus_tag="VFA54_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZS51316.1"
                     /translation="MTKRLILLALLAAVTAFPQVLSVGVKGGVPFTDAFNTAKTGRLE
                     YISDTKRYTVGPTVELHLPFRLGIEFDALYKRLDFESTSSSVDVFTRTATTANSWEFP
                     LLLKWRVFGGPIRPYVEGGPTFDKLSDISQLARTILVPNRETSASTGHPPELQNTFRV
                     GATVGAGIELGSRRFRVSPEVRYTRWGWENFQDLSGGSLLRSNDNQAEFLLGITF"
     gene            complement(4245..5174)
                     /gene="miaA"
                     /locus_tag="VFA54_10680"
     CDS             complement(4245..5174)
                     /gene="miaA"
                     /locus_tag="VFA54_10680"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZS51317.1"
                     /translation="MHSLTAILGPTASGKSELALAVAERHNGEIVNFDSIQVYRWFRI
                     GAAKLREPERRGIPHHLIDILEPNEIFTAGEFARRARQAIAEICGRGRLPVLAGGTGF
                     YLRALLDGLFPGPQRDEALRRRLAARPPERLHRLLRRLDPAAAAKIHPHDSPKIIRAL
                     EVTLLARRPITALFSEGRQKLEGFRILKIGLLPDRNALYDRINRRTEAMFAAGLVEEV
                     RQILAAGYSAEAKPFESHGYRQALQHLQGELSLREAIFYAQRNTRHYAKRQITWFRRE
                     QDVEWYRGFGDDPDLQSRVLERVGAFLAAAEAA"
     gene            5211..>6231
                     /locus_tag="VFA54_10685"
     CDS             5211..>6231
                     /locus_tag="VFA54_10685"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZS51318.1"
                     /translation="MKKTSLATGFKAAALAPRFAFLLITSVFALQVDTIPLPPPYQTP
                     SVNNGPRVVSAPAGAALHLPPGFQIEVFAEGFEAPRFMLLGPTNEILLADSAGGFFGG
                     RGAVYVLSGKERRKIIDGLDRPYGLALWNGYLYVAETTSLKRYKYDAKTMKVTGKGEE
                     IVPMHQFGSGHWTRTVLFDREGKKMYLTIGSGSNVDTGEDPRRAAINRYNPDGSGHEI
                     FASGMRNVIGLRWYPGTDTLWAAVQERDLLGDDLVPDYFTRVQQGGFYGWPYAYMGHH
                     PQPRLPAKPETVDRTLTPDVPFRSHSAPLGMAFYTAKQFPETWRGGAFVAMHGSWNSS
                     TPRGYT"
BASE COUNT         1215 a         1866 c         1948 g         1202 t
ORIGIN      
        1 tctgacccga ggtgagcctg gtccgccccg gcgatctggt atcagccgag atgcatgtgg
       61 cgtgcggccg gtgttatcaa tgccggaccg gcgcgtcgca catctgccgg cgcgtgaaga
      121 tcataggcgt cgatgcggac gggtgctttg cggaatttgt gcgcgtgccc gaaagcaacg
      181 tctggaaagt agatccggcc atcaccccgg atatcgccgc ggtttttgat cctctgggaa
      241 acgcggtgca cgcggtgctg gccgggccgg tcgcggcggc gggcatcgcg gtgatcggtt
      301 gcggtcccat cggacagttc gccgtggccg tggcccgcgc ctgcggcgcc tcgcgcattt
      361 tcgccatcga gatcaacgag aggcggctgg cggtcgcctg ccgcatgggg gccgatgttc
      421 tgattaatcc cgcgcgcgaa gatccggtcg cccgagtcct ggaagagacc gcaggcagcg
      481 gggcggacgt ggttctggag atgtcggggc atccccaagg cgtgaagaac gcctttgcca
      541 tgctgcgcat gggcgggcag gtgactctgc tcggaattcc catcaagccg gtggagatgg
      601 atctcgccgg agatatcatt tttcgtggag cgacagtggc cggcatcaat gggcggcgcg
      661 tatttcaaac ctggtaccag atggaggcgc tcctcaaagg cggccggctc gacattactc
      721 cggcgattac cgatcgcctg cccctcgccg agtttcataa agccatggat ctgctccggc
      781 gcggggaagg ctcgaagatc ctgctggaac catgaaaagg actctactcc aggcgcggcg
      841 cccgcgcctt gcgaggcgcc tgccgcctga tttccctcat aatgcatgaa tcacgccatg
      901 acgaatccat tgcaatactt gaccgatgag ctcaacgagc tccggaacaa gggcctgtac
      961 cagcggctcc ggatcttagg cggagagcag cttccggtcg ccgaatttga cggccggcgg
     1021 gtgatcaatc tttcctcgaa caattatctc ggactgacca cgcatcccaa gctgcgcgag
     1081 gcggcgatcg aggcgacgaa aaaatacggc gtggggtcgg gcgccgtccg caccatagca
     1141 gggacgatgt cgatccacat ggcgctcgaa gaaaagatcg ccgcattcaa gcacgccgaa
     1201 gcctgcgtgg ttttccaatc gggttttgcg gcgaatgccg gaacggtggc ggccgttctc
     1261 ggcaaggacg acgtcattat atccgacgag ttgaatcacg cctcgatcat cgacggctgc
     1321 cggctttccc gcgccgcgat tcgaattttt ccccataaag acgtggctgc cgccgaaaaa
     1381 attctggccg aagcggcaca gcttcccggc cgcaagcttc tcattaccga cggcgtattc
     1441 tccatggagg gcgatattgc gccgctgcct gccctggtcg aactggcgga acgatacggc
     1501 gccatcatga tgatcgacga tgctcatgcg agcggcgtgc tcgggaaaaa cgggcgaggc
     1561 accgtcgatc acttcggctt gcacggacgc gttcacattc aggtagggac gctttctaag
     1621 gcgatcggag tgctcggcgg atatgtctgc ggaagccgcg atctgatcga attcctgtat
     1681 catcgcgcgc ggccgttttt gttttccacc tcgcacccgc cggcggtcgc ggcgagctgt
     1741 ctggcggcgt tcgaggtcct cgagcaagag ccggagcttg tccagcggct ctgggagaac
     1801 acgcggtttt ttaaaaaggg tctcatgcag cttgggttca acaccggccg gagcgaaacg
     1861 cccataacgc cggtaatcgt gggcgaagca acgctggccc accagttttc acgcgagctg
     1921 tttgaggccg gcgtattcgc ccaggggatc ggatttccca ccgtgccgca gggccaggcc
     1981 cgcatccgga ccatcgttac cgccactcac agccgcgcgg aactggatca ggcgctggcg
     2041 acattggaga aggtggggaa aaagctgaag atcatctgac ccgtcaggag aaagagaagc
     2101 cactgcgaag cctccggggg cctcaggctg ccaccggcga gaaaatcggc tgccattagc
     2161 tcgatctttc ttgcaagaga ttcaggtaaa aaaccggttg ggatccggaa cgcctgggag
     2221 gttattatta ggagcgccgg cgggcgcagt ctctaagcgg ttgcgacccc cccggctgtc
     2281 caacatcggg cgagacggag gtgcctatga accggcgtga atttgcggcg tcgctggccg
     2341 cggcgcttat cgccatccgg tccgggagcg aggctgcgga gaaaaacatt tcctgggcgc
     2401 tcagcctggc gctgtgggga catctggccc ctgtgccgtt tactgagatt ctggacatca
     2461 tgaaggacac cggcttcacc ggcatccgca tgaccagctt cccgggctgc ctcaagcaat
     2521 acaacttgac cattccatcg ctgcaaagag agctcgacaa gcgcgggctt cgcatcgcta
     2581 cgatttcctt cggagggccg gccgacgacc ggaccaagca tgagcagatc gagaagagcg
     2641 cacgggaggc gctgggattc ctgaaagagt tcggagccac cgagctggtt gttttctcgc
     2701 ccgcccgcgt gccgaaggtg ctcgttccgg agcgaatcca catcgccgcc gagttctaca
     2761 atcatctggg cgatgtggcc aaagaatacg gcttccgggc ggggctgcac aatcatctcg
     2821 accagcttgc cgagtgccag gacgaagtgg aaatgttttt aaaactcacc gacccgaagc
     2881 gcttcgggtt tgcgcccgac accgcccatc tccacctgtc ggggtgcaat gtggccgaac
     2941 tgttcgaaaa atacgccgac cggctgatct ttatggatta caaagacagc agaaacacgc
     3001 ccgctgcgaa ggacatcgtt ctgccaaacg ggcaaactct gaaggccggc acggcgagcg
     3061 cgacctttat gaacagcatt tacgacctgg gcgacggcga ggtgaatttc ccgcggctgc
     3121 accgcatcct gaagcggcgc aagtacaaag ggtggatctg cgtggatctc gattacgtcc
     3181 gcgccagcgc ccgtgaaagt ttcggccggt gcatgaaata cgtgcgtgag aagctggagc
     3241 cgatctacgt ctgaagccgg ggccttcacg gctcccgaaa acatctgaag ttaccgccag
     3301 gcagcgcggt cctgcgaacg aaatagggca cgggcccgga ccgcgctgtt tcgtttggca
     3361 catcttacct tcggattcca cacgcagcga acttcggaag tagaaaccga atcgagtcag
     3421 gactccgtcg aaagtgatgg ggattgactc tcagcgcaat tgacggccga aggagcgctg
     3481 cgcgcgcctg cttggccgcc ggccggaatg cggcaccgag tatgttgcgg gactactttt
     3541 taacgggaga aaagaatgac caagcgactg atcttgctgg cgcttttagc agccgtaacc
     3601 gcttttccac aggttttgtc cgtgggagtc aagggcggcg ttcctttcac cgacgcgttc
     3661 aacacggcga aaacgggccg tctggaatat atttctgaca cgaagcgtta caccgtcgga
     3721 ccgactgtag agctgcacct gccgttccgg ctgggcatcg aattcgatgc tctgtacaag
     3781 cgtcttgact tcgagagcac cagcagctcg gtggacgtat tcacgagaac ggcgactacg
     3841 gccaactcct gggagtttcc gctgcttctg aagtggcgtg tgttcggcgg gccgatccgt
     3901 ccctatgtcg aaggaggacc tacattcgac aagctttccg acatttcgca actggcccgg
     3961 acgatcctgg ttcccaaccg ggagaccagc gccagcacgg gccatccgcc cgagctccaa
     4021 aatactttcc gggtcggcgc aaccgttggc gcgggcattg aactggggtc caggcgcttc
     4081 cgcgtttccc ctgaggtacg ttacacccgt tggggctggg agaattttca ggatttgagc
     4141 ggcggcagcc ttctgcggtc caacgataat caggcggagt tcttactcgg gatcactttt
     4201 tgaggcgcct tcgcctccgg ccgccggtga tcgccctggg caaattatgc tgcttcggcg
     4261 gccgccagga aggccccgac gcgctcgaga acgcggctct ggagatcagg atcgtctcca
     4321 aagccgcggt accactctac atcctgttcc cgccggaacc aggtgatctg ccgtttggcg
     4381 taatgccggg tattgcgctg ggcatagaaa atggcttccc gtaaggagag ctcgccctgc
     4441 aaatgctgca aggcctggcg atacccgtgg gattcgaaag gcttcgcttc agcggagtag
     4501 ccggcagcga ggatctggcg aacttcctcc actaagccgg cggcaaacat cgcctcggtg
     4561 cgccggttga tgcgatcgta gagggcgttg cggtcgggaa ggagtccgat tttcaaaatg
     4621 cggaatcctt ccagcttctg gcggccttcg gagaacaacg cggtgatcgg ccgccgcgcg
     4681 aggagcgtca cttccagggc gcggatgatc ttgggagaat cgtgcggatg aatcttcgcg
     4741 gcggccgccg gatccaggcg ccgcagcagc ctgtgcagcc tctccggcgg ccgggcggcc
     4801 aggcgccgcc gcaacgcctc atcccgctgc ggcccgggaa acaggccatc cagcagagcc
     4861 cgcaggtaga atcccgtacc gccggcaaga accggcagcc ggccccggcc gcaaatctcg
     4921 gcgatagcct gcctggcgcg gcgggcgaat tcgcccgcgg tgaaaatttc attgggttcg
     4981 aggatatcga tcaaatggtg aggaatgccg cgccgctcgg gttcgcgtaa tttggccgcc
     5041 ccgatgcgga accaccggta gacctggatg gagtcgaaat ttacaatttc accattgtgc
     5101 cgctcagcga ccgccagcgc cagttcgctt ttgccgctgg ccgtaggccc gagaattgca
     5161 gttaaggagt gcatcaggaa gcctactatg ataaaggaga gtcttccgcc atgaaaaaaa
     5221 cctcccttgc cacgggcttc aaagcggctg cgcttgcgcc gcgctttgct ttcttgctca
     5281 tcacgtccgt atttgcgttg caggtggata cgatcccgct gccgccgccg taccaaactc
     5341 cctccgtgaa taacgggccc cgcgtggttt ctgctccggc cggggcggcg ctgcatttgc
     5401 cgcccggatt ccagatcgag gttttcgcgg aaggatttga agcgccccgc ttcatgctgc
     5461 tcggccccac taacgagatt ttgctggccg acagcgccgg tggatttttc ggcggccggg
     5521 gcgcggttta cgtgctgagc ggcaaggagc gccgcaaaat catcgatggt ctcgaccgcc
     5581 cttacggcct ggcgctttgg aacggctatc tctatgtggc cgaaacgacc tccctgaaac
     5641 ggtataagta cgacgcgaag accatgaagg tgacgggcaa aggggaagag atcgtgccca
     5701 tgcatcagtt cggctcggga cactggaccc gcaccgtgct atttgatcgc gagggcaaga
     5761 agatgtatct caccattgga tcggggtcca acgtggacac gggagaggat ccgcgccgag
     5821 ctgccattaa ccggtacaat cccgacggca gcggccatga gatctttgct tccggcatgc
     5881 gaaacgtcat cggattgcga tggtatccgg ggactgacac tttatgggcg gccgtccagg
     5941 agcgtgatct gctcggcgac gacctggttc cggactactt cacccgcgtg cagcagggcg
     6001 gtttctatgg ctggccctac gcttacatgg gccaccatcc gcagccgcgc cttcccgcga
     6061 agccagaaac tgtcgaccgt acgctgacgc cggacgtgcc gttccgctct cactccgcgc
     6121 cgctcggcat ggcattctac accgccaaac agtttcccga gacgtggcgc ggcggcgctt
     6181 ttgtcgccat gcatggctcg tggaattcct cgacgccgcg cggctatacg g
//
LOCUS       DASREK010000207        22601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1579659, whole genome shotgun sequence.
ACCESSION   DASREK010000207 DASREK010000000
VERSION     DASREK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 22601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22601
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579659"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..2525)
                     /locus_tag="VFA54_10690"
     CDS             complement(426..2525)
                     /locus_tag="VFA54_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HZS51319.1"
                     /translation="MAAESRVRLSDTLYAGLSADVLTQSYRWLYTSRRLDDREIILKR
                     QNKIFFQISGAGHEAIGVAAALSLKPGVDWVYPYYRDRALCLALGVTAEEMLLQAVGA
                     ARDPASGGRQMPSHWGHAKWRIVSSSSPTGTQFLQAAGCAEACRMLNGKGSDAEIVLV
                     SGGEGSTSEGEFWEALNAACLRRLPLLFLIEDNGYAISVPIECQTAGGSISRLVSNFP
                     GLLVREVDGTDFIPSYQAMVDASAYCRAGEGPALVHAHVTRPYSHSLSDDERLYKTEE
                     ERRAEAERDPVAKLGEWLVSEGVLDAHALSIIQHEAEEEIQKAVDRALRASPPSKASV
                     KAFLYSPDIDPTSEAFSRDPEFSGESKTMVDLLNACLHDEMRRDPRIIVFGEDVADCS
                     RIQNLDKVKGKGGVFKVTAGLQRKFGNDRVFDTPLAEAAIIGRAIGCATRGLKPVVEI
                     QFFDYIWPAMMQLRNELPVLRWRSNNSFACPVVVRVAIGGYLGGGAIYHSQSGEVLFT
                     HIPGLRVVFPSNALDANGLLRTAIRCDDPVLFLEHKRLYREPYNRSPYPGPDFMIPFG
                     RANLVKSGEDLTIITYGALVYRCLQAALKVEKERGVSIEILDLRTLNPYDWQAIAESV
                     RKTSRALVAHEDCLSWGYGAEIAARIAGELFSDLDAPVARVGAQDTWIAYNPQLEEAI
                     LPQTADIEHSITRLLEW"
     gene            2655..3536
                     /locus_tag="VFA54_10695"
     CDS             2655..3536
                     /locus_tag="VFA54_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS51320.1"
                     /translation="MASQQLGTAATVQEPSAGAVQAAPESNAGLWLPVATLWWRELVR
                     FYRQRSRVVGALGTPLLFWLLIGAGFGGSFRAVEPPGAPSSAGVGVSYLGYLFPGTIL
                     LIVLFTAIFSTISVIEDRHEGFLLSVLVAPISRSALVLGKVLGGATLALLQGLTFVLL
                     APLVGIRLGAWQAAEVTAILFLISFSLTALGFYFAWRLDSVQGFHSVMNLVLMPMWLL
                     SGALFPVSGARSWIGWLMRMNPLTYGMDALSRALANRPAAPSSMGFSVGIMAAFGMLA
                     GLASLREVRRSSAAGAV"
     gene            3533..3946
                     /locus_tag="VFA54_10700"
     CDS             3533..3946
                     /locus_tag="VFA54_10700"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HZS51321.1"
                     /translation="MTFSDLPALNATLNAVSAVFLTAGYRQIRNKNVPAHRACMLAAF
                     GASTLFLISYVIYHAHAGSVRFLRQGWIRPVYFSILITHVMLAAAILPLALVTLTRAL
                     RERFDQHRQIARWTLPVWLYVSVTGVIVYLMLYRL"
     gene            complement(4026..5099)
                     /locus_tag="VFA54_10705"
     CDS             complement(4026..5099)
                     /locus_tag="VFA54_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS51322.1"
                     /translation="MARYQYIREDEPPEAEREQGKRKCKKKLRLDRFERVALALAAAS
                     LATMLVGVKSLHLSGSHHKVERPAGWRILRPPFPHLAETYDPLEELRIPVSEYSAGKR
                     LEQILVILRGPWRFGIAEMERVALALGPPATQGLGYMSYEIEDGSVRLTAIFNIRRLS
                     VIALWPNSCDPAAPDCLHAAAWGLTGGQAQNLRYEPSDDAEDPCGTLEDSTQGFYYDV
                     CRTEKPGEWSVKAVYVERVSRDMLNPVYVRDVLPPDQTQITQRPPLDQEQYQSLFQHY
                     RRAVQLSAKTLGADHVWVEYLRFEMGRMQAEAGRQKEGRTMMKAAMDDLERRLGPSHP
                     DVAAMRPWLDAFGKSEPGPRRRG"
     gene            5316..6452
                     /locus_tag="VFA54_10710"
     CDS             5316..6452
                     /locus_tag="VFA54_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZS51323.1"
                     /translation="MRWNSSSGRRSRLQGYVLVTTALTIVALIGILGLCVDLSRMYVV
                     RNELQAYADAASLAAARHLDGTSAGLTYATNVAKSYPDKWNFATTAPETVAVSFASDP
                     SGPYVANPVSPVGIKYVQVTATGTVTLYFLPSFSTLAPSAMLLSIARQEPLTVTATSG
                     QFLKSTPFSTGLLPYSPYAHNPNDPNFGFTPGQQYTLRWPPPGQENKNNACPGDANFP
                     DPSSSSSQRGFIDIGWPAGAGPNGGGSAFIREAIISNVQSHPLNVGDTLTMVMGNRGT
                     ESDALRERFSEDTDTTSTTWADYSANLQAGTANGRRLGIVPVNDPNNNDVVLGFALVL
                     LQSDICGPSNVSPCCATYISSSPVIPGIQGGSSTPGAFQLKLYK"
     gene            6458..6928
                     /locus_tag="VFA54_10715"
     CDS             6458..6928
                     /locus_tag="VFA54_10715"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZS51324.1"
                     /translation="MQSSFFHRRRGIPGRGSSRRGNAMVEFAIGFSLLIAAFTGVYRF
                     GYTFFVYNILESAVREGARYGSLEVYDGATNGATFTSWVKNMVVYTNPTPPTGATPVV
                     PGLTTDNVTVNANLDAKGIPTRITVQISNFQSSLFVTTLNKPKCSFDYMGRYTD"
     gene            6960..7403
                     /locus_tag="VFA54_10720"
     CDS             6960..7403
                     /locus_tag="VFA54_10720"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HZS51325.1"
                     /translation="MMSRLSAYHRKNRRGSAFVEGALVLTVVIFTLLGIVDLGQVLIV
                     QQALVERVRQGARWAVVNPFDATKIANVVMYNTPSPSGNAKPLFGLTTSMVSASQYAA
                     NTPEWRIVVQIHDYPFHFFSPWLKGDYTARPIMVDVSGEGQGATN"
     gene            7615..8448
                     /locus_tag="VFA54_10725"
     CDS             7615..8448
                     /locus_tag="VFA54_10725"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HZS51326.1"
                     /translation="MGYKPILNWPPAPEAWNLYEPSGVAVDHEGNVYVFNRSPHPLLK
                     LTSEGKLIKSYCEGGVFVDSHGVRIDRDDNIWAVDRNGQMIFKINQEGRILMVLGRRG
                     QKGTAHDNFNRPTDVAFAPNGDVFISDGYGNSRVIKFDKNGKYLLEWGKKGSAPGEFN
                     LPHAIVLDTQGRVYVGDRENYRVQIFDVNGKFLGQWTNVGSPWGLDLTPDKQHILLSD
                     GHNNRVLLLNLEGQILGVQGSPGKAPGEFIHAHAIASAPNGDFYVAETVNWRVQKFVQ
                     R"
     gene            8485..9267
                     /locus_tag="VFA54_10730"
     CDS             8485..9267
                     /locus_tag="VFA54_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51327.1"
                     /translation="METKNGLSRRNLLAALTAAAAASRATQALAAAAGDKANVPVISK
                     RVEKLFLGPGKEPNALQCVSDGLWILDQEDPNKVHKVRYEDGSVMVELQTESYHGSGI
                     TYGNGALWIASTGKLKTLKVDPATGKTLKSFDTPGVGPIKWGKDHHVSGAHGMEWVDG
                     KYWIAVPPSVVIYLIEPETGQIIRSIPAPGVRPHGLAWDNGYLWCVESNDRAIYKLDP
                     KDGRPVAKIQLTRQDPEPHGLSIRGGVLWYSDASSRWISKLV"
     gene            complement(10029..10703)
                     /locus_tag="VFA54_10735"
     CDS             complement(10029..10703)
                     /locus_tag="VFA54_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS51328.1"
                     /translation="MKTLLAASLAAVLLAPAAFPQVAKKANEGYQTPEGRAKVAKTLD
                     DPHRTEQMKPRQIVAALDVKPGSTVADIGTGVGFMLPYLEEAVGPGGRIYAEDIRQDF
                     LDKAEARARSGKWSNVTFVLGDDRDPHLPEGQFDLAFTLEVYHHFDYPAEMLAHISRA
                     LKPDGLLVIADFYKNRRGGQGKDMSGHIRIDKNDVIQEVESNGYRLLSARDHSDNMYI
                     LIFAKQ"
     gene            complement(10726..11826)
                     /locus_tag="VFA54_10740"
     CDS             complement(10726..11826)
                     /locus_tag="VFA54_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZS51329.1"
                     /translation="MAAAARSRALPHFYALIMAGGRGTRFWPRSRKARAKQVLPVLGE
                     KSLIQETFERLRPLIPAERFLIITNTYLRAEIARQLPEAPLSQVIAEPAQRNTAPCIG
                     LAARILLDRDPEAVMGVFPADHVISRPAAFRAVLKRAYRAAAAGRLIVLGIKPHWPET
                     GYGYIEFAKKAESGAGPVPVLRFREKPDAATARRFLKRGNFYWNSGMFVWKAAVIDRA
                     LREFLPETSAALSGIRGRPGSLEFERSLARFYPRTENISIDYAVLEKSPNLAGFRCEE
                     LGWNDVGSWGAVHGLLPADSQGNVARGHALFYKSKGSYVDAPGKLAALVGVEDLIVVD
                     TPDALLVARRDRAQDVGEVVKILEKQERHDLL"
     gene            complement(11834..12625)
                     /gene="lepB"
                     /locus_tag="VFA54_10745"
     CDS             complement(11834..12625)
                     /gene="lepB"
                     /locus_tag="VFA54_10745"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZS51330.1"
                     /translation="METGKTTVVDTERGPEAPPPPEPQRGMIAEWTITIILLLFGTTT
                     LMQAFVIPTGSMEDTLLIGDHLIVDKLAYAPGGSVMQHLLPYEEIHRGDIIVFRYPVD
                     IRQTFVKRAIGIPGDHIRIRNKQLFLNGKEVLEPYKYHKTDYIDSYRDNFPSPPNTFV
                     YKPGIDMLEHHVVNGEVVVPPGHYFAMGDNRDLSLDSRYWGFVPRENIIGKPLIIYWS
                     YDAPTDQLQSPLISFEHARDLALNFFSKTRWRRTFRLIHGYNLNP"
     gene            complement(12645..16769)
                     /locus_tag="VFA54_10750"
     CDS             complement(12645..16769)
                     /locus_tag="VFA54_10750"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZS51331.1"
                     /translation="MRFLLRPLAAMLALAIVFLAANRGRRLASAAESSPWLPTAADQR
                     GAVQHEISLPLRGLTPGGIYSISFSLSSPNEFVSQNSAGPGSEPKLVVAIRQGSRDLA
                     FKILHLGDPDLYALVRLDSGDAEVRLTPANPPPAGFHYHLLVNHWAESARLAAEPNNS
                     WQQANPIELGTTVFGSGDLSPYIPPDPHSEGAPPQTNDDWFCFDFRETNPKLVFFTLD
                     LMERDNIPVDVAVFRVENGKPVAYERGADPVTPPHEVQALPGNKFTTRVLNEAGRYYI
                     RVQARHPFYKLRTAVYDVPPYRDPRQAVQTAVDYIIRAGDSWHANTPRKGGVYDRVAN
                     VHQETSLCVACHPTHFSQRAQLYALRNGYAVTGRQQLQFLAERFYNNPRPFYGYEAQG
                     ASWARVISAPANVLGRMAALLNIYEKEVTGEHREKFFRGISEYLKLYYKDRDTLPPDE
                     SNGNTPLVSTYEVAWYAWVAAPELRPRLEQLIAQGTFENGQIKNVCDLCYQTLALAEI
                     NREKYAGKIRRNAERLLALQRPSGQWALDFQPEAPEAEFQTGHALWALAAAGYPADHP
                     QIAKGLQYLLNRQQQFGGWFDPLQSYENFRTPFRETQMAVLALSAFYKGPAPAGGKAG
                     DSGNARAAMARKRTSPTLPPDHAGAALEWMDSIWRLPSDTDLQRVERAARSNDALVRQ
                     QAAECLGRLAYAPAVPLLGSLLGDESKMVQRTAAWALRQIISRRREGAREVEEALSAR
                     NERIRWGATRVFATHFSYVVDQVHPDPLLALLYDPSPVIRMQAIKGLWQWWFWDARGE
                     SRSRIEDAILSAMAEPQHPWVERNLREAAYNIADENVRYLYNNWVALLPKNALRDRAV
                     RGRLAVEQELAEKFARVLETGGDRQKKILLAGLGQFPLRRADSYPLNAQLVRKEEGLK
                     YTRIGNDIEQILFLGRSADRMAQVLLPLIQSSDPEMRREATLAAYMVREIRTKQSYGG
                     SRRYGWDDVVRLAGFHEENRRKLALAVIERLEDPDSSVRAIARDIYSDFTQDFDGPGR
                     SRVEQVLLRLLESSNPESRAAALWIIHDAPAFPPDSPILPLVRQAVLAGKQGVAAVAA
                     LQACPSLFGDPQIVEAVRRAIESENPAMQRAGLELILRNQPKMQDPVLAAAFQKAVAA
                     TDSSRKRLLLDIIDAHREIAETEAGAGIVNLALRSGETSLAAAAVNLVKEERRLFRRR
                     DTQTLLERAIDDGKLSSTLRTSIAELLGKTAASNSIPAAAPKTAVNLDYPYFRERVEP
                     LLEARGPDGNACVDCHSTHTLFHLNRPRPGAPGEAQVRQNYESALKVAAPGEPESSLI
                     LRKPTSDAAVEGVLGGGLSHGGGIRWPAGSWQYDTILNWIKGARSDQKSAKLSN"
     gene            complement(16782..17603)
                     /locus_tag="VFA54_10755"
     CDS             complement(16782..17603)
                     /locus_tag="VFA54_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51332.1"
                     /translation="MSGRAGAARGAGFSRPSPGARSSGAAVSPARISPAGVAYTPNIY
                     GSVSGFGNVVFPGTGHAPGAYNPFTIVGPGFGANLANTVVSPGFGASLGSTVGGFSTG
                     RGFRRFGRSSSTTVIVPYAVPVYVPAYPYAEPNAGYVDANAVPPDTQVGAAYPPVAPP
                     QVIYVVPSQPPQPQAPSSGGVVTYVVPPRDNAGGQPSTGVTVTAPPNGISRPIYLIAL
                     KNNTIYSATECWVEDDTLHYVTPEGAHNQVSLDQVDVDFTTRLNRERGIEFRLQQ"
     gene            18213..19265
                     /locus_tag="VFA54_10760"
     CDS             18213..19265
                     /locus_tag="VFA54_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZS51333.1"
                     /translation="MNPRVKNLLFLFVANLLGFLLVFGGAEFVVRWRAEHGFSPAWRS
                     LFTASSPLSDLGTGNVLIADPDLGYRFNPALPGVNSIGIRGPEIAPAKAGGKERIVVL
                     GDSVAAPANGFVTLLREALHGQAEVINAAVPGYTTYQERRLLERDLLRVHSDLVLLQY
                     CLNDNHQFLHRFDAQAHMLFTEEARRAVLPSGSGLFAVLARHSYLALRIRLALLSLRA
                     FHPAYPWEDQPDVAAAWQDDPWNTFAGHLAAMRDEVAANGGRLTVLMAPYRPQFDSAL
                     LARNARYVLKPQEKMTQICSELGVPLLDLYPVFTAHGGGRLFFDNYHFNAAGHSIAAE
                     SLLGFLRSHNLLNPGL"
     gene            19365..21737
                     /gene="lptC"
                     /locus_tag="VFA54_10765"
     CDS             19365..21737
                     /gene="lptC"
                     /locus_tag="VFA54_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZS51334.1"
                     /translation="MKKTRFALLVLLISAIAAVVGGYYAAEKRNQLLAPAKPAPLPSD
                     LHSIANNWSWSQSSKDHPVVEAHAQDFRQIKDSSRFELGGVEMKIFSKAGDTYDLVRS
                     KQAEFDQEVERLYSEGEVTIVLGLPADSPPPPGKRYVEIRTSGLTYDNKTGISATDRP
                     VHFTLENGDGDSVGALYDANKRYLWMKKNPVIIGAGPQAGMRIRAGELMYYEAEQKVE
                     LRPWSKLERGGQGVEAGNSVVYLEDGNLKRVDAERGRGWDIGPDRQVHFQGDRIEVNF
                     TPQQTLASASGIGGAEVVSQSESGVTKITGGRVDLEFRTPAGAAESELTAAYVRDHGR
                     LESTPLAQNGTQAETKVLTAEVIQVEMRPGGRDLQKLQTHAPGRLELIPNQPDQWKRT
                     LTAERMTAEYLPGNRPGSLRAAGSVFLRSDPPAAAVKAARPAGGAPQTAPALPRLTWS
                     DDLAASFDPETGDIRELHQWNNFRYEEGMRRAQAADARFDAASDRVTFENRARVWDES
                     SMITAARIVLDQQQDRLHAEGDVTSTHLGGISAAPHPGPTAVDSGSIFSPEKPVHATA
                     QQLDSERHNHLLHYRRSARLWQDGNSIEAQEIEVDREKKTLRAAGSVISVLTEDDQPG
                     GDSAPSGGQSEKPPASARPPGPRLVRVTSDSLVYTDQDRRAYYAGHVILKRERMLIRA
                     AALEAYLRPQEQVENGESRIDRAVAEGKVEIVETPLEKRTPRKASAEHAEYYSNEEKI
                     ILRGGNPVLEQPGRGSTRGSELTYYLDDDRLLVNGRPGARSQTREKIKHD"
     gene            21832..22557
                     /gene="lptB"
                     /locus_tag="VFA54_10770"
     CDS             21832..22557
                     /gene="lptB"
                     /locus_tag="VFA54_10770"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZS51335.1"
                     /translation="MGTLRTDSLSKSYRGRKIVDDLSVSVSLGEVVGLLGPNGAGKTT
                     SFYMIVGLISADSGRVWLNDDDITDLPMYLRARRGISYLPQEPSVFRKLSVEENLLAI
                     LETLSIPEHERREKLETLIEQLGLQNVRHSKAYQLSGGERRRVEIARSLVISPQFLLL
                     DEPFSGIDPKQVLELQHIIFGLKESGIGILITDHNVRETLRVTDRAYIINNGRIFRAG
                     PPTELGRDPEVKRVYLGETFSLR"
BASE COUNT         4386 a         6823 c         6947 g         4445 t
ORIGIN      
        1 cgtaattttg ccctgggatc tcgccggcaa atacgcggtc cagggtctcc atatcggagt
       61 agaaaaaact ggaggagctg taaattggcg tcgtgaccgg gacgaaagat ccgggtttct
      121 tgcggtcgcc ggcgtgaatc gcgaggctgt caagtttcat ggcgggtctt gagcaggatg
      181 ccgacaggtc tccctgagcg gccgccggcg gcgaacacgg cgcgcagccc gcggcggatg
      241 agcgactgca cgcagcgcgc aatcgctttt cgaacaccct gttaggcgat tatagcaacc
      301 gggagtgttc gcgaggcgga tggccagccc gcccgtgcaa gcattccgtc cgaatacgct
      361 atcttcggtt cagcggctgc gccaaacaag cgaacgctgc gcacaagggg cggcgaaggg
      421 cggggttacc actccagcaa gcgcgtgatg gaatgctcga tatctgccgt ttgcggcagg
      481 atggcctcct cgagctgggg gttgtaggcg atccacgtgt cctgggcgcc gacgcgggcc
      541 accggagcgt ccagatcgga gaacagctcg cctgctatgc gggcggcgat ctcggcgcca
      601 tagccccagg agaggcagtc ctcatgggcg acaagggctc gcgaggtctt gcgcaccgat
      661 tccgcgatgg cttgccaatc gtaagggttg agcgtccgca gatcgaggat ctcgatcgag
      721 acgccgcgct ctttttccac cttgagcgcc gcctgcaagc agcgatatac gagagcgccg
      781 taggtgatga tggtgaggtc ttcgcccgat ttgaccagat ttgcgcggcc gaaaggaatc
      841 atgaaatcgg gaccgggata gggagaccgg ttgtagggct cgcgatacaa gcgcttgtgc
      901 tccaaaaaca gcaccggatc gtcgcagcgg atggcggtcc gcagcaggcc gttggcatcc
      961 agcgcattgg agggaaacac gacacgcagt cccggaatat gcgtaaacag gacctccccg
     1021 gattggctgt ggtaaatcgc cccgcccccg agatatccgc caatagccac gcgcaccacc
     1081 accggacacg cgaagctgtt attcgaccgc cagcgcagca ccggcagctc gttgcgaagc
     1141 tgcatcatgg ccggccagat gtagtcaaaa aactgtatct ccacaacggg cttgagcccg
     1201 cgcgtcgcgc agccgatggc ccgcccgatg atcgccgcct cggcgagcgg agtgtcaaag
     1261 acacgatcgt tgccgaactt gcgctggagg ccggcggtga ctttgaagac gccccctttg
     1321 cctttcacct tgtccaaatt ctgaatccgg ctgcaatcgg cgacgtcttc gccgaatacg
     1381 atgatgcgcg ggtcgcgccg catttcgtca tggagacagg cgttcaacag atcgaccata
     1441 gtcttcgatt cgcccgaaaa ttcaggatct cgcgagaatg cctccgaggt ggggtcaatg
     1501 tcgggcgaat acagaaaagc cttcaccgaa gctttcgaag gaggcgaagc tctcagagca
     1561 cggtcgaccg ccttctggat ctcctcctcg gcctcgtgct ggatgatgga aagcgcatga
     1621 gcatccagca cgccttcgga aaccagccat tcgccgagtt tcgccaccgg atcgcgctcg
     1681 gcttcggcgc gccgctcttc ttcggtcttg tagaggcgct cgtcatcgga aagcgagtgg
     1741 gaatacgggc gcgtgacgtg cgcgtggacc agggccggtc cctcgccggc gcggcagtag
     1801 gccgatgcat ccaccatagc ctgataggaa gggataaaat cggtcccatc cacttcccga
     1861 accagcagcc cggggaagtt cgaaaccagc cgggaaatgc tgcctcccgc cgtctggcac
     1921 tcgataggaa cggaaatggc gtacccgttg tcttcgatca aaaacaacag cggcaggcgc
     1981 cgcaggcagg cggcgttcag cgcctcccag aactctcctt cgctggtgga tccctcccct
     2041 cccgacacca gaacgatttc cgcgtcgctt cccttcccgt tcagcatccg gcacgcttcg
     2101 gcgcaaccag cggcttgcag gaactgcgtg ccagtggggc tcgaagagga cacaatgcgc
     2161 cacttcgcat gtccccaatg agaaggcatc tgccggccgc cggatgcggg atcgcgggcg
     2221 gcgccgacgg cctgcaacag catttcttcc gccgtaaccc ccagcgccag gcataaggcc
     2281 cggtccctgt agtacggata tacccagtcc acgccgggtt tcagagagag agccgccgct
     2341 acgccgatcg cctcatggcc ggcgccgctg atctgaaaga aaatcttgtt ctggcgcttc
     2401 aggatgatct ctcgatcatc caggcgccgg gacgtataca gccaccggta agattgcgtc
     2461 aggacgtcgg cggagaggcc tgcgtatagc gtgtcggaaa ggcgaacacg gctttcggca
     2521 gccatcgagt tcttttatca gtgtatcgaa gctgtttccg cccgggcaac agaaaacagt
     2581 tcgccgccga acgcgggcgc tgagctgtgc agccacgacg cggagcgagg ccgccgcatt
     2641 gtagaatcag agggatggcc agccaacagt tgggaacggc cgcaacggtg caagaacctt
     2701 cggccggcgc ggtgcaggcg gcgcccgaat ccaatgcagg cttgtggctg ccggtggcca
     2761 cgctgtggtg gcgggagctg gtgcgatttt accggcagcg gagccgcgtg gtgggcgcgc
     2821 tgggaacacc gctgctgttc tggctgctga tcggagcggg ttttggcgga tcgttccgcg
     2881 ccgtggagcc gcccggcgcg ccctcctctg ccggcgtcgg cgtgagctac ctggggtacc
     2941 tgttccccgg cacaattctg ctgatcgtgc ttttcacagc cattttttcc accatatcgg
     3001 tgatcgaaga ccgccatgaa gggtttctgc tgtcggtcct cgtagcgcct atttcgcgtt
     3061 ccgcgctggt gctgggaaaa gtattgggag gagcgacgct agccttgctg caagggctga
     3121 cgtttgtgtt gctggctccg ctggtgggga tccggctggg agcgtggcag gcggccgagg
     3181 ttaccgcaat cctgttcttg atttcctttt cactcaccgc cctgggattc tacttcgcct
     3241 ggcgtctcga ttcggtgcag ggttttcaca gcgtgatgaa cctcgtactg atgccgatgt
     3301 ggctgctgtc aggagccttg tttccggttt ccggcgcgcg ttcgtggatt ggatggctaa
     3361 tgcggatgaa tccccttacc tatggcatgg acgccctgag ccgcgccctg gcgaaccggc
     3421 cggcggcgcc ctccagcatg ggcttttcag tgggcatcat ggccgctttc ggcatgcttg
     3481 ccgggctggc cagtttgcga gaggtgcggc ggtcttcggc cgccggcgca gtgtgacctt
     3541 ctccgacctt ccggcgctca acgcgaccct caacgccgtc agcgcagttt ttctgacggc
     3601 gggctaccgc cagatccgga acaaaaacgt gcccgcgcat cgggcttgca tgctcgcggc
     3661 attcggcgcc tccacgcttt tcctgatttc ctacgtgatc taccacgccc acgccggatc
     3721 ggtgcggttt cttcgccaag ggtggatccg gccggtctac ttcagcattc tcattaccca
     3781 cgtgatgctg gccgccgcca ttctgccgct ggcgctggtc acgctgacgc gggccttgcg
     3841 cgagcgcttc gaccagcatc gccagattgc ccgctggacg ctgccggtgt ggttgtatgt
     3901 ctccgttacc ggcgtgattg tttatttgat gctatatcgg ttataggtgt tgcgatgcgg
     3961 accgccgcgg ccggccgggt acaggaggcg gttccggagc ggtcatctcc ccgcagcctt
     4021 gcggatcaac cacggcggcg gggtccgggt tcggatttgc caaatgcatc cagccagggg
     4081 cgcattgcgg caacatcggg gtgggacggg ccgaggcggc gttccagatc atccattgca
     4141 gctttcatca tggtgcgccc ttctttctgc cgccccgctt cggcttgcat gcgccccatt
     4201 tcgaaccgca ggtattccac ccagacgtgg tcggctccga gcgttttggc gctgagctga
     4261 accgcgcggc gatagtgctg gaacaagctt tggtattgct cctgatcgag aggcgggcgc
     4321 tgcgtgatct gcgtctgatc gggtggcaga acgtccctga cgtaaacggg gttcaacatg
     4381 tctcgactga cgcgttccac gtaaacagct ttgacagacc actcgccggg tttctcagtc
     4441 cggcaaacat cgtagtagaa gccctgggta gagtcctcca atgtgccgca cggatcctcg
     4501 gcgtcatccg aaggctcgta gcgcaggttc tgcgcctggc cgccggtcag gccccatgcc
     4561 gccgcatgaa ggcaatcggg agccgcgggg tcgcaggagt ttggccacaa cgcgattacg
     4621 ctcagacgcc ggatgttgaa tatcgccgtc agcctcacgc tgccatcttc gatttcgtac
     4681 gacatatagc ccaatccctg cgtggcggga ggtcccagcg ccagcgccac gcgttccatt
     4741 tccgcaatgc cgaaacgcca gggcccgcgc aagatgacca ggatttgttc caaccgtttg
     4801 cccgccgaat actcggatac tggaatcctc agttcttcca gcgggtcgta tgtctcagcc
     4861 aggtgaggga aagggggccg caatatgcgc catccggccg gacgctccac tttgtggtgg
     4921 ctgccggaaa gatgcaggga tttcactccc accagcatcg tcgccagcga ggcggccgcc
     4981 agcgccagcg ccacgcgctc aaatcgatcc aagcgcagct ttttcttaca tttgcgcttg
     5041 ccctgctcgc gctctgcctc cggaggctca tcctcgcgaa tgtactgata acgagccatc
     5101 gctggtgatg ttcgcaattc ttagcggcga aggaaaacgg gtgaaagccc gaagtgcgtc
     5161 tgttaatcgc cttaatcgaa aaggcgaacg gagtaagcgc ccaatcttag atgtcgtagg
     5221 cgttatttct tgcagccgat gggcaagcgc tctatagcag tttagtcttt gtttcctaaa
     5281 aatgactaga gagcagaatt tgcttaggag gcttcatgcg ctggaattcg tcctccggtc
     5341 ggcgtagccg gttgcaggga tacgttctgg tcactacagc gctcaccatt gtcgctttga
     5401 tcggcatact cggtctgtgc gttgatctct cccgaatgta cgtagtgcgc aacgaactgc
     5461 aagcttacgc ggatgccgca tcgcttgccg ccgcccgcca tcttgacggg accagcgccg
     5521 gcctgaccta cgccaccaac gtggccaaga gctatccgga taaatggaat ttcgcgacca
     5581 ccgctccgga aacagtggcc gtttcttttg cgagcgaccc ttctggtccc tacgttgcca
     5641 atccggtcag tcccgtcggg ataaagtacg tgcaagtgac cgccactggc acggtgacgc
     5701 tgtatttcct gccgtcgttt tcgacactgg cgccctcggc gatgctgctg agcatcgccc
     5761 gccaggagcc cttgactgtg acggccacct cgggacagtt cttgaagagc acgcccttct
     5821 ccaccggcct gcttccgtat tcgccgtatg cacacaatcc gaatgatccg aattttggct
     5881 tcacgccggg acagcagtac acgttgcggt ggcccccgcc cgggcaggag aacaagaaca
     5941 acgcctgtcc cggtgacgcc aatttccctg atccctccag ttcgtcctcc cagcgaggct
     6001 ttattgatat cggttggccc gcaggcgccg gccccaacgg cggaggatcc gcgttcatcc
     6061 gcgaagccat catcagcaac gtccagtccc atcctctcaa tgtcggcgac accttgacga
     6121 tggttatggg aaatcgcggc accgaatcgg atgcgctgcg agagcggttc agcgaggaca
     6181 ccgataccac ctcgacgacc tgggccgact attcggcgaa tttgcaagcc ggaacagcca
     6241 acggccgccg cctcggaatc gttcctgtca acgatcccaa caacaatgac gtagtgctcg
     6301 gattcgcgct ggtgcttctc caatcggaca tttgcggtcc cagcaacgtc tctccgtgct
     6361 gcgccaccta tatcagctcg agccccgtga taccggggat tcagggcggc agcagcacgc
     6421 ctggcgcatt ccaactcaag ctatacaagt gagaattatg caaagcagct tttttcaccg
     6481 tcgcaggggc atcccaggcc gcggttccag ccggcggggc aatgccatgg tcgagttcgc
     6541 catcgggttc agccttctga tcgcggcttt caccggagtc taccgctttg gctacacctt
     6601 cttcgtttac aacatcctgg aaagcgccgt gcgcgagggc gcgcgatacg gatcgctgga
     6661 ggtctacgac ggagccacca acggggcgac ttttacaagc tgggtcaaga acatggtcgt
     6721 ctatacgaac ccgacgccgc cgaccggagc cacgcccgtg gttcccggac taaccaccga
     6781 caacgtcacc gtgaatgcga atctggatgc gaaaggaatt ccaaccagga tcacggtcca
     6841 aatatcgaat tttcaaagca gccttttcgt cacgacgctc aacaagccca aatgcagttt
     6901 cgattacatg gggcgctaca ccgactgatg acgggtaaag cagaggcagg ggagagcaaa
     6961 tgatgagccg cctatcagcc tatcaccgga agaaccggcg gggcagtgca ttcgtagagg
     7021 gagcgctggt gttgactgtg gtgattttta cgttactggg catcgttgat ttggggcagg
     7081 tcctgatcgt ccagcaggcg ctggtcgagc gcgtgcggca aggcgcccgc tgggccgtag
     7141 tcaatccctt cgacgcaacc aaaatcgcca acgtagtgat gtataacacc ccttctcctt
     7201 ccggcaacgc caaaccgctg ttcggcctta cgacttccat ggtcagcgcc tcgcaatacg
     7261 cagcgaacac gcccgagtgg cgcattgtag tgcaaatcca tgattacccc ttccatttct
     7321 tctcgccttg gctgaaggga gactacacag cccggcccat catggttgac gtttcgggcg
     7381 agggccaagg ggccaccaac tagagcgtcc ggcgcctgca aattttcctt ctgcaagttg
     7441 aagcggtcct ccgcaagcta gtataattcg accgctgcat cggcgagact tcctgtctat
     7501 tggcgccgag gaggtgcgat gttgtctgcg cgtgtacgga ctgggtttct gcggatttct
     7561 ctcgctctct gcttcctggg ctgctcgttc ggccaacagg ccagtcttcc ccctatgggg
     7621 tataagccta ttctgaactg gccgcctgcg ccggaagcat ggaacctgta cgagccttcg
     7681 ggcgttgcgg tggaccacga gggcaacgtt tacgtgttta atcgcagccc gcacccgttg
     7741 ttgaagctga ccagcgaggg aaagctcatc aagtcctact gcgagggcgg cgtgttcgtg
     7801 gacagccacg gagtgcgcat cgaccgcgac gacaacatct gggccgtgga ccgcaacggc
     7861 cagatgatct tcaagatcaa tcaggaaggc cggatcttga tggtgttggg ccgccgcggc
     7921 cagaaaggga cggctcacga caatttcaat cgcccgacgg atgtcgcttt cgcccccaac
     7981 ggcgacgtct ttatttccga cggctacggc aattcgcggg tcatcaagtt cgataaaaat
     8041 ggaaagtatc tgctcgaatg gggaaagaag ggatcggcgc cgggcgaatt caatttgcct
     8101 cacgcgatcg tgctcgatac tcaagggcgc gtttacgtgg gcgatcgcga gaattaccgc
     8161 gttcagattt tcgatgtcaa tggaaagttt ctggggcaat ggacgaacgt cggctccccg
     8221 tggggcctcg acctgacgcc cgacaagcag cacattctgc tctccgacgg gcacaataac
     8281 cgcgtcctgc tgctgaatct ggaagggcaa atcctcggcg tgcaaggcag cccgggcaag
     8341 gctccgggag aattcattca cgcgcatgcg atcgcatcag cgcccaatgg ggatttctac
     8401 gtggcggaga ccgtgaattg gcgtgtccag aaattcgtcc aaagatgact ggcgcgtcct
     8461 gaagctcgtg agaaggaggc tggtatggag acgaagaacg gcctgtccag gcgcaatctt
     8521 ctggcggccc taacagcagc cgccgctgcg tcaagggcaa cgcaagctct ggcggcggcc
     8581 gccggggaca aggcgaacgt cccggttatc tcaaagcggg tcgaaaagct ttttctcggt
     8641 ccgggcaagg aacccaacgc gctgcaatgc gtatccgacg gactgtggat tctcgatcag
     8701 gaagatccga acaaggtgca caaagtccgc tatgaagacg ggtcggtgat ggtcgaattg
     8761 cagaccgaat cttatcatgg cagcggcatt acctatggca atggagcgct gtggatcgcc
     8821 tccaccggga agttgaaaac gctcaaggta gatccggcta cgggcaaaac tctcaaatcg
     8881 tttgacactc cgggagtagg cccgatcaag tggggcaagg atcatcatgt cagcggagcc
     8941 cacggcatgg agtgggtgga cggaaaatac tggatcgccg tgccgccttc ggtggtgatt
     9001 tatctgattg agcccgaaac cggccagatc atccggagca tccctgcgcc cggggtacgg
     9061 ccgcatggtt tggcctggga caacggctat ctgtggtgtg tggagtccaa tgaccgggcg
     9121 atttacaaac tggatccaaa agacgggcgt cccgtggcca aaatccagct aacccgccag
     9181 gatcccgagc cgcacgggtt aagcatccgc ggcggtgttt tgtggtactc ggatgcttcg
     9241 agccgctgga tttcgaaact ggtgtagccc gctcaggagc agcgctgtgc atgtccggcg
     9301 acaggcagcc caggcgcccg ccgcggatgc ggacagtccg actaagttga ttttatttgc
     9361 cagaagagac gctcgctttc gggcgcggcg ctcggaggcg gcggtatagc acggggtggg
     9421 gtatgcgtca cggatcggct tcgcaacgct tcggccgacg gggcggatcc ttttccacaa
     9481 acgaagccca gccagaaaaa cgcgacaaac aagagggcca ggatcagtat ggctcctgcg
     9541 gcctgcccaa tcttggcaag ggcgttcgta ggggtctgta tcaccctctg attctgcact
     9601 cgccggcaaa aaccccggaa tagccgagca gtcacatttc tgagctggta cgaaacaggc
     9661 ggaagaggct gagctaaggg gtactgaagt ttcggcggag cgccggtttt gatggattcg
     9721 gtccctcctg tgcgaggtct gcctttatca tccccggcgc agcgcaggct gtcccttcgt
     9781 gattgatccg gcggaactcc gcccccggtc actctcagcg cgccccgctc attctgatcc
     9841 cgccttggtc attctgagtg aagccgagaa gccggcttgg tggttgtgaa cgaagaattt
     9901 tgatgggcgg cgaacaggcg atccctcgct tctgcctggg gtggccgcaa gacgggcggg
     9961 caataaattg gatcacgccg gacgaccgaa ccggtcgttt cgcccgtggt gacacgcggg
    10021 atgatcgtct attgtttcgc gaaaatcagg atgtacatgt tgtcgctgtg gtcgcgggcg
    10081 gagagcagcc gatacccgtt ggactccact tcttggatga cgtcgttctt atcgatccgg
    10141 atgtggccac tcatgtcctt tccctgcccg ccccgcctgt ttttatagaa atccgcaatc
    10201 acgaggaggc cgtccggttt caatgcccgc gaaatatgcg ccagcatttc ggccgggtag
    10261 tcgaaatgat ggtagacctc cagcgtaaac gccagatcga actgcccctc cggcaggtgg
    10321 gggtcgcgat catcgccaag cacgaaggtc acgttggacc acttgccgga tctggcgcgg
    10381 gcctcggcct tgtccagaaa atcctggcgg atgtcctcgg cgtaaatgcg cccgcccggc
    10441 ccgacggcct cttccaggta cggcagcata aagccgacgc cggtcccgat gtcggctacc
    10501 gtgctgccgg gcttaacatc gagagccgcc acgatctggc gcggcttcat ctgctccgtg
    10561 cggtgcggat cgtcgagggt tttggcgacc ttcgcgcggc cttccggcgt ctggtagccc
    10621 tcgttggctt tcttagcgac ttgagggaac gccgcaggcg ccaggagaac ggcggccagg
    10681 gaggctgcaa gaagcgtttt catggaccac actatagcag gttgctcaca gaagatcatg
    10741 gcgctcttgt ttctccagga ttttcaccac ctcgcccaca tcctgagcgc ggtcgcggcg
    10801 cgccaccagc aatgcgtcgg gcgtatccac tacgatgagg tcttccacgc ctaccagcgc
    10861 agccagctta ccgggagcgt ccacgtagct tcccttcgac ttgtaaaaaa gggcatggcc
    10921 acgcgccaca ttgccctggc tgtcggccgg cagcaaacca tgtacggcgc cccagcttcc
    10981 tacgtcgttc cagccgagtt cttcgcaccg gaacccggcc agattgggcg atttttctaa
    11041 cacggcataa tcaatcgaaa tgttttcagt gcgcgggtag aacctggcca atgatcgttc
    11101 gaactccagg ctgccggggc ggccgcgtat cccggacaag gcggctgagg tttcggggag
    11161 aaactcgcgc agtgcgcggt cgatcactgc ggccttccag acgaacatgc cgctattcca
    11221 ataaaaattg ccccgcttga ggaagcggcg ggccgtcgct gcatccggct tttcacggaa
    11281 gcgcagtacg ggaacgggtc cggctccact ctctgctttt tttgcgaact cgatatagcc
    11341 gtagccggtt tccggccagt gaggcttgat tccgagcacg atcagccggc ccgctgcggc
    11401 ggcccgataa gcccgtttta ggaccgcccg aaaggcagca gggcgggaaa tcacgtggtc
    11461 ggccggaaaa acgcccataa ccgcttccgg atcgcggtcg agcaggatgc gcgcggcgag
    11521 gccgatgcaa ggggccgtgt tgcgctgcgc gggctcggcg atcacctggg aaagaggcgc
    11581 ctccggcaac tgccgcgcga tttcggcgcg cagatacgta ttcgtgatta tcagaaatcg
    11641 ttcggcggga atgagcggcc gcaggcgctc gaaggtctcc tggatgagcg atttttcgcc
    11701 gagcacgggc agcacctgct tggcccgagc cttccggctg cgcggccaaa agcgcgtgcc
    11761 ccggcctccg gccatgatga gcgcataaaa atgtggcaaa gcgcgagagc gagcggccgc
    11821 tgccatgcgc agtctacggg ttgaggttgt agccgtgaat caggcggaac gtgcgccgcc
    11881 accgggtctt gctgaagaaa ttcagcgcca gatcgcgggc atgttcaaag ctgatgagcg
    11941 ggctctgcaa ttggtcggtg ggcgcgtcgt acgaccagta gatgatcagc ggcttcccta
    12001 taatgttttc ccggggaaca aagccccagt agcggctgtc gagcgacaga tctctgttgt
    12061 cgcccatcgc aaaataatga ccgggcggga ccacgacttc gccgttgact acatggtgtt
    12121 cgagcatatc gatgcccggt ttatagacaa atgtgttggg aggagaggga aaattgtcgc
    12181 ggtaagagtc gatatagtcc gtcttgtgat atttgtaagg ctccaaaact tcctttccgt
    12241 tcagaaaaag ctgcttgttg cgtatgcgga tgtgatcgcc cggaattccg atcgcacgtt
    12301 tgacgaacgt ctgccggatg tccaccggat aacggaagac gatgatgtcg ccgcgatgga
    12361 tctcctcgta aggcaacaag tgctgcatca ccgaaccgcc cggcgcatac gccagcttgt
    12421 ccacgatcag gtgatcgccg atgagcaagg tgtcttccat cgacccagtg gggattacga
    12481 aggcctgcat cagcgtggtc gtgccgaaca acagcaggat gattgttatc gtccactcgg
    12541 caatcatgcc gcgctggggt tcggggggag gcggcgcttc ggggccgcgt tctgtatcga
    12601 caaccgttgt ctttcctgtc tccacggagg gacctcgtaa cgaactaatt actcagttta
    12661 gcggactttt gatcggaacg tgcgccttta atccaattca gtatcgtgtc gtactgccac
    12721 gagccagcgg gccagcgaat gccgccgccg tgagaaagac cgccccccaa cactccctcg
    12781 accgcggcgt ccgacgtcgg cttgcgcaat atcagacttg attcgggttc gccgggggca
    12841 gcgactttca acgccgattc gtaattttgc cggacctggg cctctccggg agcgcctggg
    12901 cgaggacggt tcaggtgaaa aagagtgtgg gtgctgtggc aatccacgca ggcgttgccg
    12961 tccggcccgc gagcttcgag gagcggttcc acgcgttccc gaaagtaagg gtaatcgaga
    13021 ttgaccgctg tttttggcgc cgcggcggga atcgaattcg aggcggccgt tttgccgagc
    13081 agttcggcaa tggacgttcg cagggtggag ctgagcttcc catcgtcaat cgcgcgttcc
    13141 agcaaggttt gcgtatcacg gcggcggaag aggcggcgct cctcttttac gaggttcacc
    13201 gcggctgcgg ccaaactcgt ctcgccgctg cgcaacgcca gatttacgat gccggcccca
    13261 gcctcggttt cggcgatttc ccgatgcgca tcgatgatat cgagcagcag gcgctttcgg
    13321 gacgaatctg tcgccgcaac tgccttttgg aacgcggctg cgaggaccgg atcctgcatc
    13381 ttgggctggt tgcgaaggat cagctccaga ccggcccgct gcatagccgg attttcagac
    13441 tcgatcgcgc ggcgcacggc ctcgacgatt tgcggatcgc cgaacagcga cgggcaggcc
    13501 tgcaaggccg ctactgccgc cacaccctgt tttcctgcga gcacagcctg gcggacgagc
    13561 ggcagaatcg gtgaatccgg ggggaaggca ggtgcgtcat gaatgatcca gagcgccgcc
    13621 gcgcgtgatt ccggattcga gctttcgagc agccgcaaga ggacctgttc aacccggctc
    13681 cgcccgggcc cgtcgaaatc ctgggtgaag tcggagtaaa tatcccgcgc gatagcccgc
    13741 acggagctgt ccggatcctc aagccgctcg ataaccgcca gcgcgagctt gcgccggttc
    13801 tcttcgtgaa agccggccag ccgcactacg tcatcccagc catagcgccg ggatccgcca
    13861 tagctttgct tggtccggat ttcccgcacc atgtatgcgg ccaaagtcgc ctcacgccgc
    13921 atttcgggat cggacgactg gatcagaggc agcaaaacct gagccatccg gtcggcgctc
    13981 cgcccgagaa agagaatctg ctcgatgtca ttgccgatgc gggtgtactt taaaccctct
    14041 tcctttctga ccagttgcgc attgagtgga tagctatcgg cccggcgtaa gggaaactgg
    14101 ccgagaccgg ccaaaagtat tttcttctgg cggtcgccgc ccgtctcgag aacccgcgcg
    14161 aatttctcgg ccagttcctg ttctaccgcc aggcggcctc ttacagctcg atcgcgcagc
    14221 gcgtttttgg gcagcagcgc cacccagttg ttatacagat agcgaacatt ttcatccgcg
    14281 atgttgtagg cggcctcccg gaggttacgc tccacccacg gatgctgagg ttctgccata
    14341 gccgagagaa tcgcgtcctc aattcggctg cgagactccc cgcgcgcatc ccagaaccac
    14401 cactgccaga gacccttgat cgcctgcatc cggatcacgg gcgacgggtc gtaaagcagc
    14461 gccagcaacg gatcgggatg cacctggtcc acgacataag aaaaatgcgt cgcgaagacg
    14521 cgggtggcgc cccatcggat gcgctcattc cgggcggaga gggcttcctc gacttctcgc
    14581 gccccctccc tccgtcggct gataatctgg cgcaacgccc aggcagccgt gcgttgcacc
    14641 atcttgcttt catcgccgag cagcgatccc aaaagcggaa ccgcaggagc atacgccagc
    14701 cggccgaggc attccgccgc ctgctgccgg actaaagcgt cattcgagcg cgccgcccgt
    14761 tcgacgcgct gaagatccgt atcggagggc agccgccaaa tcgagtccat ccactccaat
    14821 gcggcgccgg cgtgatccgg cggaagggtt ggactggttc gcttccgagc cattgcggct
    14881 cgggcatttc ccgaatcacc cgctttcccg ccggcgggcg cagggccctt gtagaatgca
    14941 gagagcgcga ggacggccat ttgagtctcg cggaaaggcg tccgaaaatt ttcgtaagat
    15001 tgcagcggat cgaaccatcc accgaattgc tgctgccggt tgaggagata ttgcaagcct
    15061 ttggcgatct gcggatgatc ggccggatag ccggcggcgg ccagagccca taaggcgtgt
    15121 ccggtctgga actcggcttc gggagcctcg ggctggaaat ccagcgccca ctggccgctc
    15181 gggcgctgca aggcgagcag ccgctcggcg ttgcggcgga tcttcccggc gtatttctcc
    15241 cgattgatct cagccagggc cagcgtctgg tagcacagat cgcagacgtt cttgatttgt
    15301 ccgttttcga aagtgccctg agcaatcaac tgctcgaggc ggggccgcag ttcgggcgcc
    15361 gccacccatg cataccaggc cacttcgtaa gtgctcacca gcggcgtgtt tccattcgat
    15421 tcgtccggcg gcagcgtgtc gcggtctttg taatagagct tgaggtattc cgagattccc
    15481 cggaaaaatt tttcccggtg ctccccggtc acttcctttt cgtaaatgtt gagcagagcc
    15541 gccatgcgtc ccagtacgtt ggcaggagcg ctgatcacgc gcgcccacga cgccccctgc
    15601 gcctcgtagc cgtaaaatgg gcgcgggttg ttgtagaagc gctcggcgag gaactggagc
    15661 tgctgccgcc cggtgaccgc atagccgttt cgcaaggcat aaagctgcgc ccgctggctg
    15721 aagtgcgtgg gatggcaggc cacgcacaac gatgtttcct ggtgtacgtt cgccacgcgg
    15781 tcgtaaacgc cgcctttgcg cggggtgttg gcatgccagg agtcgcccgc ccggatgatg
    15841 taatcgaccg cggtctgcac ggcctgccgc ggatcgcgat atggcggaac atcatagacg
    15901 gcggttcgca gcttatagaa agggtgccgc gcctgcaccc gaatgtagta gcggccggct
    15961 tcgttcaaga cacgagtcgt gaatttgttt ccgggcagcg cctggacttc gtgcggcgga
    16021 gtgaccggat cggcgccgcg ttcataagca acgggcttcc cgttctccac tcgaaaaacg
    16081 gctacgtcca cagggatgtt gtcgcgttcc atcagatcga gtgtgaagaa aaccagcttc
    16141 ggattggttt cccgaaaatc gaagcagaac caatcatcgt tcgtttgggg tggcgctcct
    16201 tccgaatgcg ggtccggcgg tatgtaaggg ctcagatccc ccgagccgaa gaccgtagtc
    16261 cctaactcaa tgggattcgc ctgttgccag gagttgttgg gctcagccgc cagtcgggcg
    16321 ctttccgccc aatggtttac gagcagatgg tagtggaaac cggcaggggg cgggttcgcc
    16381 ggagtcagcc ttacttcggc atcccccgaa tccagacgga ctagcgcgta aagatccgga
    16441 tctcccaaat gcaagatctt gaacgccaaa tcccggctgc cttgcctaat ggcgaccacg
    16501 agctttggtt cggaaccggg gccggccgag ttttgggaaa cgaactcgtt cgggctggat
    16561 agggaaaagc tgatgctgta aattccaccg ggcgtcagcc cgcggagcgg caggctgatt
    16621 tcatgctgga cggcgccgcg ctggtcggct gctgtgggaa gccagggcga gctttccgct
    16681 gcggatgcaa ggcgccgccc gcggtttgcg gccaaaaaaa caatcgccag agccagcatc
    16741 gcggccaatg gccggagcag aaatctcata gccggatttt gctactgttg cagccgaaac
    16801 tcgatgcccc gctcgcgatt cagccgcgtc gtgaaatcca cgtccacctg gtcgagagaa
    16861 acctggttat gcgccccttc aggggtcacg tagtgcagcg tgtcatcctc aacccagcat
    16921 tccgtggctg agtagatcgt gttgttcttc aaagcgatca aatagatcgg ccggctgata
    16981 ccgttaggcg gcgcggtgac cgtgactccg gtggagggct ggccgcccgc gttgtcgcgc
    17041 ggcggaacca cgtacgttac gaccccgccg ctggatggag cctgcggctg cggcggctgg
    17101 ctcgggacta cataaatgac ttgcggcgga gcaaccggcg gataggcggc cccaacctgc
    17161 gtgtccggcg gcacggcatt tgcatccaca tacccggcgt ttggctcggc atagggataa
    17221 gccggcacat agacgggcac agcgtagggg acaatcaccg tggtcgaact ggaacgcccg
    17281 aatcgccgaa agccgcgccc agtgctgaat ccgccgacgg tgctgccgag actggcgcca
    17341 aaaccggggc tgacgacggt gtttgccaga ttagcgccga agccggggcc gactatggtg
    17401 aacggattgt aggcgccggg cgcatgcccc gtgccaggga acactacatt gccgaaaccg
    17461 ctgaccgagc cgtaaatatt gggagtgtaa gcaacgccgg ctgggctgat gcgggccggc
    17521 gaaacagccg cccccgagga tcgggcgcct ggcgatgggc gcgaaaatcc ggcgcctcgg
    17581 gcagccccgg cgcggccgct catggccgcc cctgcgtggc cgccggatgt tcctccgaat
    17641 ccgccgttcc actgtgcgaa ggacacaatg gaaaatgaag ccaaccaagc caaccatgcc
    17701 gtccttctca cagcgattct ccttgccttc agcttagcgc tgttcgaacg ccgctacaag
    17761 tagctatcga gggacgttgg cggccccgga agcgttccag ccggaaacct ctatttgcgg
    17821 aatttcctcc ggcggcctgt tggaccggag cggtcctcaa gcctgctttt ggcttcccgg
    17881 aatacgagcc atagcgcccc aagctcaaag ccgagggcct ttatcggctc gtcttgatag
    17941 aagaagcgcc agaagcaggc cgtcaaaatc acaagggcgg ccagcttcgc ccagcccggc
    18001 atggatcgga gcatagccca aggtccaggc cgcgccaatg cagcaaaaca ctgagcccag
    18061 cgcggacgaa attcttcctg gaattactcg tttaccgtta tgtcaaccgg cataggccgc
    18121 cggcgataag cccggtgcta aaattgcgcc gagcgccttt gctcttcgcg gcggtgggca
    18181 ggccgagcgc cgggcggttg ttttcatctc cagtgaaccc tcgggtcaaa aatttactgt
    18241 ttctgttcgt tgccaatttg ctgggcttct tgctggtttt cggtggcgcc gaatttgtgg
    18301 tgcgctggcg agcggagcac ggcttttcgc cggcgtggcg gtctttattc accgcttcct
    18361 cgcctctatc tgatctcggc acaggcaacg tgctgattgc cgacccggac ctgggctatc
    18421 gcttcaatcc cgcgctgccg ggagtgaact caataggcat ccgcggcccg gaaatcgccc
    18481 cggcgaaggc cggcgggaaa gaacgcatcg tcgtgctggg cgactcggtg gccgcgcccg
    18541 cgaatggatt tgtaactctg cttcgagaag cgctgcacgg acaggcggaa gtcatcaacg
    18601 cggccgtgcc gggctataca acttatcagg agcggcggtt gctcgaacgc gatttgctgc
    18661 gcgtccactc cgacctggtg ctgctccaat attgtctgaa cgacaatcat cagtttctgc
    18721 accgcttcga cgcccaggcg cacatgcttt ttaccgaaga agcgcgccgc gcggttcttc
    18781 catcaggctc cggactcttc gccgtgctgg ccagacacag ctatttggcg cttcgaattc
    18841 gcctggcctt gctctccctg cgcgctttcc acccagctta cccttgggaa gaccagccgg
    18901 acgtggcggc agcctggcag gatgacccgt ggaacacctt tgcgggacat ttggccgcca
    18961 tgcgggatga ggtagctgca aacggaggcc ggctaactgt tctcatggcg ccctaccggc
    19021 cccaattcga ttcggcgctg ctcgccagga atgcccgcta tgttctgaag ccgcaggaaa
    19081 aaatgacgca aatttgcagc gaactgggcg tcccgttgct cgatttatac cctgttttca
    19141 ctgcacacgg cggcggccgg cttttcttcg acaattatca tttcaacgcc gccgggcaca
    19201 gcatagccgc agagtccctg ctcggattct tacgctctca caatttgctg aacccgggcc
    19261 tgtaatggcc gccggtgatt tccggcggaa ggtaaaaaag cctttttcct cgggagccgc
    19321 gcttttcggc ctccgtgctt accctgatat catgatcgat gagaatgaaa aagacgcggt
    19381 ttgcgctgct cgtgctgctg atttccgcca tcgccgcggt ggtaggaggc tattatgcag
    19441 ccgaaaagcg caatcaactg cttgctcccg ccaagccggc tccgctgccg agtgatctcc
    19501 attcgatcgc caacaactgg tcgtggtcgc aaagttcgaa agatcacccg gtcgtcgaag
    19561 cgcacgccca ggactttcgc cagatcaagg attcgtcccg cttcgaactg ggcggagtgg
    19621 agatgaagat tttcagcaaa gccggggaca cctacgatct ggtccgctcc aagcaggccg
    19681 agttcgatca ggaggtcgag cgcctttaca gcgaaggaga agtcaccatc gtgttggggc
    19741 tgcccgccga ttccccgccg ccgcccggga agcggtacgt ggaaatccgg acgtcaggcc
    19801 tcacctatga taacaagacg ggtatcagtg cgaccgaccg ccccgtgcat ttcacattgg
    19861 agaatggaga cggcgactcg gtgggagcgt tgtacgacgc caataagcga tacctgtgga
    19921 tgaagaaaaa tcccgtaatt atcggggcgg ggcctcaggc gggaatgcgc atccgcgcgg
    19981 gcgagctgat gtattacgag gccgagcaga aggtggaact gcggccatgg tcgaagctgg
    20041 aacgaggcgg gcaaggggta gaggccggta attcggtcgt ttacctggag gacggcaatc
    20101 tcaagcgagt ggatgctgag cgggggcgtg gatgggacat cggcccggac cggcaagttc
    20161 attttcaggg cgacaggatc gaggtgaatt tcacgccgca gcagacccta gccagcgctt
    20221 ccggcatcgg cggcgccgag gtcgtttcgc aatccgagtc gggagttacc aagatcaccg
    20281 gcggccgcgt ggatctcgag ttccgcactc cggccggggc agccgagagc gagctcactg
    20341 cggcgtatgt ccgggatcat gggcggctcg agagcacgcc gctggcgcag aacgggacgc
    20401 aagcggaaac taaggtgctg accgccgagg tgattcaagt cgagatgcgg ccaggcggca
    20461 gagatctcca gaagcttcag acgcacgcgc ccgggcgcct ggaactgatt ccgaaccagc
    20521 cggaccagtg gaagcgaacg ctcaccgccg agcgcatgac ggcggaatat ctgccgggaa
    20581 atcgtccggg ctcgttgcgg gccgccggca gcgtatttct gcgcagcgat cctcccgccg
    20641 ccgccgtcaa agctgcgcgc ccggccggtg gggctcctca gacagcgccg gcgcttccgc
    20701 gactgacgtg gagcgacgac ctggccgcct cctttgatcc cgaaacaggc gatatacggg
    20761 aactgcacca atggaacaat tttcggtatg aggaagggat gcggcgggcg caagccgccg
    20821 acgcccggtt cgacgctgcc agcgatcgtg taacctttga aaatcgcgcc cgcgtctggg
    20881 acgagagttc gatgatcacg gcggctcgca ttgtcctcga ccagcagcag gaccggctac
    20941 acgcggaggg cgatgtaaca tctacacacc taggagggat ttcggcggcg ccgcatccgg
    21001 gcccaaccgc ggttgattcc ggatcgatat tctccccgga gaagcccgtg catgccaccg
    21061 cgcagcagct cgattccgag cgtcacaatc acttgctcca ctatcgccgc agcgcccgcc
    21121 tttggcagga cggcaacagc atcgaggctc aggagattga agtggaccga gagaagaaaa
    21181 cgctgcgcgc cgccgggtcg gttattagcg tcctcaccga ggacgaccag cccggcggcg
    21241 acagcgcccc ttcgggcggg cagtccgaga agccgccggc ttcggcccgg ccgccgggcc
    21301 cgaggctggt ccgggtcact tcggactccc tcgtttacac tgaccaggac cggcgcgcct
    21361 attacgccgg ccatgtgatc ctcaagagag agcgtatgtt gattcgcgcc gccgcgctgg
    21421 aggcctatct gcgcccgcag gagcaggtgg aaaacgggga atcacgaatc gaccgggccg
    21481 ttgccgaagg caaggtggaa atcgtggaga cgccgctgga gaagcgcaca cccaggaaag
    21541 cctcggccga acatgccgaa tattattcga atgaggagaa gatcattctg cggggcggca
    21601 accccgtgct cgagcagccc ggccgcggat ccactcgggg gtccgaattg acctactacc
    21661 tggacgatga taggctgctg gtgaacgggc ggccgggagc ccgctctcag acccgagaaa
    21721 aaataaagca cgactgatgt ttcccggcgc ctcagccgga catcgatacc cctggaagta
    21781 acgaatccgt gctcgtttgt gtctataagc tgtggcgttg acgccgcatt catgggtact
    21841 ctgcgaacag actccctctc gaagagctat cgcggccgaa agattgtaga cgatctctcc
    21901 gtttccgtca gcctcggtga agtagtgggc ctgctgggcc ccaatggtgc aggcaagact
    21961 acgtcttttt acatgatcgt tgggctgatc agcgccgact cgggccgggt gtggctcaat
    22021 gatgacgaca ttaccgatct gcccatgtat ctgcgcgccc ggcgcggcat cagctacctg
    22081 ccgcaggagc cgtcggtttt tcgtaagctc tccgtcgagg aaaatctgct cgcgattctc
    22141 gaaactctct ccatcccgga gcacgagcgg cgcgagaagc tggaaacctt gatcgagcag
    22201 ttggggttgc aaaacgtacg ccacagcaag gcctaccagc tctcgggcgg agagcgccgg
    22261 cgcgtggaga tcgcgcgttc gctcgtgatt tctcctcaat ttctgcttct tgacgaacct
    22321 ttttccggaa tcgatcccaa gcaggtgctg gagctccagc acatcatctt cggcctgaaa
    22381 gaatccggca tcgggattct gatcacagat cacaacgtcc gggaaacgct gcgagtaacc
    22441 gaccgcgcgt atatcatcaa caacgggcgc atcttccggg cagggccgcc caccgaactg
    22501 ggccgggatc ctgaagtgaa gcgggtgtat ctcggcgaaa cattctctct ccgctaaaac
    22561 gctcgaccgc ggtcccattc gccgtgcgcc gcacgtcgct t
//
LOCUS       DASREK010000208        29756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2080664, whole genome shotgun sequence.
ACCESSION   DASREK010000208 DASREK010000000
VERSION     DASREK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 29756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29756
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2080664"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /gene="der"
                     /locus_tag="VFA54_10775"
     CDS             complement(<1..1137)
                     /gene="der"
                     /locus_tag="VFA54_10775"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZS51336.1"
                     /translation="MERSERPTVVIVGRPNVGKSTLFNRITGVRRAIVGNEPGITRDR
                     IHSPAEWRGRPFELIDTGGILVGEEEEIPVQILKQARIAIEQAWHIIFVIDGRTEITG
                     ADRELARLLLQTGKPLTLAVNKVDAVSLESGIGDFYSLGIERVVPVSAEHNKGVDELL
                     DIVTEHFPAADASEVGKTTGLGSFKKPVQVAIIGKPNAGKSTLLNRLAGAERAIVSPT
                     PGTTRDAVEALVRRDEVAYNFVDTAGIRRKGKTKLMAEKLSVVMARRHIRLAEVVLLL
                     IDGVEGVTALDATIAGYAQESGKPIVIVVNKWDLARTQTSRQEFTARLREKLKFLDYA
                     PVEFVSALTGSGTERLFPAIQRVYHAAHRRIATGELNRFFGSLDL"
     gene            1204..1647
                     /locus_tag="VFA54_10780"
     CDS             1204..1647
                     /locus_tag="VFA54_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HZS51337.1"
                     /translation="MALYYPLFLNIQGRTAVVVGGGRVAARKIAGLLEAGARVRVVAP
                     EISRQVAAHEIERRPYRTGDLKGAVLAFAATDSRDVNHAVAVEAARRRIPVNVADSAA
                     ESTFIVPARITRGEMQIAISTGGRNPRLAGQMRRRLEAELRWAAK"
     gene            1655..1957
                     /locus_tag="VFA54_10785"
     CDS             1655..1957
                     /locus_tag="VFA54_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2103 domain-containing protein"
                     /protein_id="HZS51338.1"
                     /translation="MRLRTAEGKLKIEHSIIEGLRPALERLLRHNPEIKSVIPGVIRP
                     VRDARGAIRIRITTPTQTGWKVIAFAAGARQEVFVNTGLTREQLEAALASALGESA"
     gene            complement(2052..3122)
                     /locus_tag="VFA54_10790"
     CDS             complement(2052..3122)
                     /locus_tag="VFA54_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51339.1"
                     /translation="MEATFHGRRAVQLHNREIRVSVVYGGGHVAEICLLENGVNPLWV
                     PPWPSIEPSDWDPRRTPEYGIDRESRLLSGILGHNLCFDYFGAPSDEEAAAGLGVHGE
                     APVVRWEIETISESELLCKALLPVAQMRFERRLRLGSGGVVLFTETAENLSAADRAVG
                     WTEHVTLGPPFLSRGRTIFAASATRSKTYETEFAQGKGRMRVGAEFDWPLCPANDGGC
                     LDLRQIPDVPVSAGFTTHLMDPAREQAWFTAYDPDAQVLFGYVWRRADFPWLGIWEEN
                     HCRTHKPWNGRTLTRGMEFGVSPFPEPRRRMIDRGSLFGTPGYRWIPARSKITAAYCA
                     FIRRSEKPLVEAEWRGEEVICR"
     gene            3174..3656
                     /locus_tag="VFA54_10795"
     CDS             3174..3656
                     /locus_tag="VFA54_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZS51340.1"
                     /translation="MASRSVNKVILLGHLGRDAEIKYTPNGASRCTFTLATSRRWKDQ
                     QTGEWKEETDWHNVVLWRSENVAPHLQKGKQVYVEGRLQTRSWEDKDGNKRYTTEIIA
                     DNLILLGGRGGEAIGAGDEDPASASASGPRSAGAGRGFTAAPQSDMASQEISDDDVPF
                     "
     gene            3962..4798
                     /locus_tag="VFA54_10800"
     CDS             3962..4798
                     /locus_tag="VFA54_10800"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZS51341.1"
                     /translation="MTRKVFCLSLTAVAAGLLAQQQAPKAAAPSGLPKIQALIITGQN
                     GHDWRATTPVMRKILEDTGRFEVRVTEEFRGAGPETLSPYELVILNYYERRRPELRWG
                     ERADNALLNFVRSGKGLVMYHFSMAAFDGWEEYEKLSGANWRPNNGHHSARHNFTVDI
                     RDADHPITRGMKSSFPETSDELYANLKWQPPNTFHVLATAWDDHSLYQGKARQPIPGP
                     GKDEPMLWTVNYGQGRVFATALGHDPEAMKSHGFITTFQRGSEWAATGNVTIPVPPEL
                     AK"
     gene            4944..5318
                     /locus_tag="VFA54_10805"
     CDS             4944..5318
                     /locus_tag="VFA54_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51342.1"
                     /translation="MADNFLVPETKIEANGAGPALALGPSAGRTLLLTLGITKILEQQ
                     SLDVSIWGSVDGSDWGPKPLLSFPQKFYVGSYTLLLDLSDKPEIQFLQARWTVNRWGV
                     GSTKPMFLIYIFVEVASGVKTA"
     gene            5587..8109
                     /gene="gyrA"
                     /locus_tag="VFA54_10810"
     CDS             5587..8109
                     /gene="gyrA"
                     /locus_tag="VFA54_10810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZS51343.1"
                     /translation="MDDIEKPPQGPTQLPLPPDGGDKSLVPIDIEDEMRKSYLDYAMS
                     VIIGRALPDIRDGLKPVQRRILYGMYESGLLPNRPYRKCANTVGYVLGNYHPHGDAPV
                     YEALVRLAQPFSMRYPLVDGQGNFGSVDGDPPAAMRYTEARLARIAVTLLDDIDKETV
                     DFRPNYDDRTVEPEYLPALIPNLLINGAQGIAVGMATNIPPHNLGEIIEAAILMVQNP
                     KVGLDKILEKVPGPDFPTGGFILGRQGIIDAYTKGRGHLRLRARAAIERVGKDKEQIV
                     VTEIPYQVNKARLQEHAAQLVNEKKIEGIADIRDESDREGMRIVFELKRGEQPEVVLN
                     NLYKQTQMQTGFGIIMLAIVNGQPREVGLAEALRLFIDHRIDIVRRRTDYLLRKARER
                     EHVLLGFRKALENLDAVIKLIRASRSPAEARTGLMERFEFTERQAQAIIELQLQRLTG
                     MERQKILDELAEIQKRIQEYLEILGSDKVLRNVIVKELREVQKEYADPRRTEIIDDTG
                     EIQLEDLIADEDVAITVTHNGYMKRTAVDTYRKQSRGGKGRIGMGTRTEDFVEHLLIA
                     STHSYILIFTNLGRVYWLKVYEIPDVGAAGKGKNVANLVNLQPNETVKAFLPVKQFTP
                     GVYVVMVTKKAIIKKCELTVFDNPTSRGIIAMSLEEGDELIAAKLTDGSQQIFLGTHN
                     GKAIRFPESKVRPMGRPAYGVRAMTLDPGDFLVGMEAVGLEGLILTVSENGYGKRTAL
                     EEYRITSRGGKGVINMKTTERNGKVVAILNVKESSNLFIITKDGKILRLEASEIREAG
                     RSTQGVRLVRLEEGDRVAAASVIPEEESNGEGPNGQGALLTQ"
     gene            8303..9391
                     /locus_tag="VFA54_10815"
     CDS             8303..9391
                     /locus_tag="VFA54_10815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS51344.1"
                     /translation="MLFAFLTSTPMDLLRGSGTFAGIQGLAEGLRGLGHTVRVLTPNF
                     PCFHYTTRRLIYNRRLAARVDALRPDIVIGFDMDGYRYARRSPRAPFVASIKGVIADE
                     LTNESGATRWMMTVQAKREKINVLHADLVLATSQYSAAKIAEHYGIEKFKVHIVPELI
                     DLEGWRARFAAHPAAAPERFTVLSVCRFYPRKRLEDLIAAAALLPGTEVRIAGNGPEA
                     KRLHRLGPHISWLGDVTAGQLAEEYNRCHVFCLPSVQEGFGIVYLEAMAAGKPIVAAR
                     AAAVPEVVRDGVEGILVEPRDPAALAAALARLRDDAGLRNTLSRNATARAADFQREKV
                     CRRFLDAVEKLRRSPPPCSRQATTARHQ"
     gene            9486..10565
                     /locus_tag="VFA54_10820"
     CDS             9486..10565
                     /locus_tag="VFA54_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51345.1"
                     /translation="MANCTASAIRRPVKLCLPFAAFLLLEACSLMPARPARAPQRCRN
                     LIRNANWERQGEGWRLPAFAKLEQPPSSGGVASIVVEGAADGAIAQIIPRPPELSGLG
                     VVAVGYARVELSHTLDSTDDDITSRLWEGSVNLVNGYSRNGEDFASPYVRIRFTGREI
                     ASGCWKRFVTGVIPAGRARLLYPHFAFWGARLAPGVKLHLAALSLVEAPADDNGEPER
                     WVDCANLPAPSPVLAAPTQQQWAESLAPNGSFDSFHMQTRPLAAAHSLRELVLEARYH
                     QGSLIADRYLISVTQDGSDPRLSPTSHIVTALARRGVEDFEAVVKVRADAQPLRIAIA
                     GAAEVKDGLRAQPAILPLEWQRPRL"
     gene            10572..12263
                     /locus_tag="VFA54_10825"
     CDS             10572..12263
                     /locus_tag="VFA54_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51346.1"
                     /translation="MPSAEREDRRFRWIAGFSYPLLLSVYLIYWSHADYFVDDWFLIK
                     HFRHAAGWKGVAGFAAAAAENRVYQVFRMQWLSILYGFLITALGGYSPRFNFAALLLL
                     HAASAWLLSQAVRRAGFERGLAFLAGVFYLLAPMAHFGLLTYLANPFFVLSTFWVLLL
                     LWFSSGARRPGMGELAFASACAVAALFSGEQVFLLVWAIVPLAALFFRKKPIPAAWPA
                     AGAIWAAASAALAVYLLWINRLPVRQTGIHRRYEWTSARFASNLLQIGGELRQLSGVT
                     SDSLFHFSLHKTEDALALAGALLVTALVWKWRGGDRENGRLRRLFGFAVGGAALAYAP
                     VMWIAGGFPRFRYHYAPAPYLGLAAAVAVWACGRSRWTRWSPAALAGLATGFLIFNAA
                     ADLRQSWIPESEQQHNLEVYMRQVKNIAPGDIFVVSGTPPQIGTAPHFSMHSTISASA
                     FVELVTGLEPLEAYLDFWNLHGRLVRDPDDLEHFLSESDLARTHVLMCSYDGRFSSLD
                     WIAIEIGTDSFRLLPLKGVSDSGNARRQIYSREQLALAQWPVYFPKIVRPRVAAR"
     gene            12435..14291
                     /locus_tag="VFA54_10830"
     CDS             12435..14291
                     /locus_tag="VFA54_10830"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HZS51347.1"
                     /translation="MHLDSKALSLKQPEVAVGAGRLRVLDRLAPALLVPLIIFNLGYM
                     YEGPNYALLSPWIIRLSRPELLARDWFANTIPHHANFVRFLAWSSAALPLPAVLLSLH
                     VAALFLLLWTAYGFAARLFGDKRIFYAAVFLLLRWGTEGLGGNSLWADYLVPHNAAAP
                     VCLLAFYLSLSGRPLSAALAAAAATWIHIQLGVLTMAVLGGEMLVSCVWGGGRSPARS
                     PVRKGASLGPMFTVGGIYAAAVAPTFLKQWGLYVMTASPLTARQFLDLHAILRQPHHL
                     IPSTWPLADYARFFLILGMGGLAVSWRNAQHRKIVVWSFIILAFCVAGSVFVEIIPIK
                     LVIKMQLFRMTIFLKFFAVLFAAQFLIRTLDLGRWEEKCCALAILAIQNFFVAGACAV
                     LIPTLRKNRRWAWTLSIAAAGCLALSGIAGAGVGGWLQHALRMAPRGIEIGLITLGFL
                     GAIVWRNGRLLPAGLMILAAALPLASGIPSFHYDHPPIDDWYRFCRQVRAATPADAVF
                     ITPPVMGGFQMFAERAEVADFKSTPSIEHDLVEWKHRMDDLAGGAGLHCSGWAECSAA
                     LTSGYAHLTRRNFMVLAQKYRAEYVVTPRPEPWLGFSEVLRVGDFVLYRLPA"
     gene            14397..15344
                     /locus_tag="VFA54_10835"
     CDS             14397..15344
                     /locus_tag="VFA54_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZS51348.1"
                     /translation="MLSRRQFLRLCSATCATSAAGVAAAKPRFRIGITTNTRNGGSWD
                     KDMLLSFREAAEAGYHNVETFYGYIRPWWDKPQELKSQMDRLKLGFVTISNGGPMKMQ
                     FENPSVAQQLIDEHVQLALWSKKWFGCDHLKANTGGRRPEGTTSDDLAQMARTMNEIG
                     KRLADQGLKFGVHAHLWTQLQTRSEIDHIMDATDPRYVFLVLDTGHITMAGMDPVELA
                     RTYVSRIVEFHLKDTKPEDKGGHKGPTPQREGYTAIGKRIFFELGKGGVDFPGLLSVL
                     KQNNWDGWLTVELDSTDTTPKDSAMISRRYLENVLHLQV"
     gene            16215..16766
                     /locus_tag="VFA54_10840"
     CDS             16215..16766
                     /locus_tag="VFA54_10840"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HZS51349.1"
                     /translation="MARPTAPVPGWYAIYTRSHFEKRVAADLTAKHIQNYLPVVEQVH
                     QWKDRKKLIEVPVFPSYVFARFRDSNKTRLEVLRVAGAVRILGHGESIEPIPDWEIES
                     IRRLLRANVRCFAHPFLREGAWVRVKRGALKDLEGLLVRVKSKTRLVLSISLLSQSVA
                     TEIDISDVEVIRPGSRTNGEGWR"
     gene            16763..17800
                     /locus_tag="VFA54_10845"
     CDS             16763..17800
                     /locus_tag="VFA54_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HZS51350.1"
                     /translation="MRRRFIVPVLLLVAPLFGQDRGNRREQESQPLPQSEAAEPALPG
                     ADYSRGAGAANAAAAPRAGALAFSNYKMGPEDLLAVTVLDSPEFTRQVRVSGDGTIRM
                     PLLKRAIPAAGKTSAELEQAISRELVEEGMLVDPSVSVTVLEFNSKPVSVSGAVRSPV
                     VFQAVKPLSLVEAIARASGLAENAGSDILISVPARNGRPARTVTVPVKALQQPSDPGA
                     NFLLEGGEEVKVTPAGLVYIVGGVNKPGAVLMNTEGPFTLLKALALSGGTTPTAGSKA
                     FLLRNSANGQKKEIALNLTKLMRRQEPDLPLENNDVVFIPDSTMRKISQTGMSSAVSA
                     VVFAMGTLILR"
     gene            17797..20025
                     /locus_tag="VFA54_10850"
     CDS             17797..20025
                     /locus_tag="VFA54_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZS51351.1"
                     /translation="MSSYPRPLPGPGQYPEESNYYPIQPTGEPAAVPLNHYLWVLKRH
                     KWRILGFVSAVVAAVLLYSLQLTPVYESVATLEIDNQVQMFEVGNSGLQFDNRNFDAV
                     VATQLELMDSPAVAQQVIRELHLDQNPDFNPALRNKATSDALAAGLPLSGAGAGVPDA
                     LPGLSVRRRPDTYLIEVHYRSPNPELASAIANSAAQAFVQQGFKSRYQNAAELSKWLN
                     MQLDELRAKLERAQQKLQAFEKQYNVVNPEDRTNILNLQLQKLQEELTKAQAERLRKE
                     SEYQSVESGNLEALAISEQGAPLMKIADRLESLETALAEAGSQYGPNHPIYKRQQAQI
                     ERVKALLEANKQKVLKRLKADYEQALAREKALSAAVDQQKQEVDRLSERAVEYGILKR
                     EVDSQTKLYDELLKKINEATINASIKATNLRLASLATPSKTPVFPRTKVYLLLAALLS
                     TTVAVGVALAADYLDRTLRSVEQVEQWLSVPVLANLPRVSGQKIPSLLLGTPDEESNS
                     TALAKSSIPLTEALNILRTSVLLSSPGQERRMVLVASAAPAEGKSTVATGLALALAQQ
                     LQNGDRVLLVDSDLRRPTIHTIFGLSNRTGLSTVLEGQTGFAEAVLPSGIAPNLMVLT
                     AGPAPRYSSELLTMHMAKLLENLRPEFRYIVIDSAPLLICADSTILSTMVDGVVIVAR
                     AGETPREAVASALRQLRRVRANILGLVLNQVRLPDTPGYGGYYGGYYGNYRRDEKDDE
                     DA"
     gene            20018..21487
                     /locus_tag="VFA54_10855"
     CDS             20018..21487
                     /locus_tag="VFA54_10855"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HZS51352.1"
                     /translation="MPETAGALRLALPPAFAPAGQSAGLDSLAAAPRRAALLAEAERL
                     GVLPAVARRLVSDRHPRVREIAARNLWLAREQGRVLKLLADAGVRATAVKGVNLARRL
                     YPDASWREVDDIDLLVDRSDAARACAVLCGSGLTPNHRWTPEALSRQLSRPLFLAPEL
                     VFTSPSGAMVELHWDWPGGRSAPLDPFVQPEHYLVYLCRHAAKHFWSSMKWLCDLQLF
                     VSRFGATVDWELFWRVAGENGAETGCAASLELCRRAFGGLPAPGLAQRLSRNARKLVN
                     QAESVISNPAADRPHPVWTQIQLAPWRFRPALVRTWILPQPQDWSRAREQQQPSWQTV
                     RRRFGHLWQRWAFHKIRKLSAKDWGILLEAYFTLIAAECARRLVPARWLLRAAKGRLS
                     SREAGPQAWTLEQLRRIAWLVEAAANRQPVTMRCLTRSIALSWMLARRGVRPALQIGV
                     KNDSGKLQAHAWVEWQGHALQEPVNQAGEYVALDPLAFR"
     gene            21491..21802
                     /locus_tag="VFA54_10860"
     CDS             21491..21802
                     /locus_tag="VFA54_10860"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZS51353.1"
                     /translation="MKNAKECFSVDFSARYQADAEVTFQRLDEATVIVHLGTGRIHHT
                     NLTGSRIWELLAEGRSLEDIYNTLLDEFDVPPEQLRRDLESFVQELSAEKMICNREQG
                     A"
     gene            21799..23673
                     /locus_tag="VFA54_10865"
     CDS             21799..23673
                     /locus_tag="VFA54_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZS51354.1"
                     /translation="MSALSLATNAWLGVVRKESGPRGAVPRTADGRIELGAAVFSLPD
                     PKEANTTSRAVEGGRICVFTGFLFNAEDLRRELGWDEERPCASQAEIVLAGYKHWGDG
                     VLSKLEGVFGLAIWDQAKETLLCGRDPMGLHPFYYSLAGGELIFSWDSETIRLHPKVP
                     RDLNRVVLAEHLNYRWLKQEETFFQAIRRLPGGYGLTFARGRIEVFRYWDPAPPGRPV
                     NWVTREELEQFPALLRKSVERTMKYGPIGIFLSGGFDSISIAAWASEIAPNRNWATPK
                     AFSLVFPEGKFSEEPVQKAVAQTLGFPQRFGYMTDYVAGRGLLGRLLDLAGTCPVPTT
                     DVFKPPYLDLLRMAKSDGCDIVLTGEGGDEWLTVTPAYAADLIRTGNFIELTRLLKTI
                     LHCWNLPTLPAIRNVLWTNGLRMLLRDWGWRNMPKAALCRRRRLIPKAIPEWLAPDPA
                     LRRELVERFENSDREIREDTFYFMGMREFLAHPLLAMTFEERFYRDRLAGVPTLHPYW
                     DLPLVDFLMRTPPELLNSGGRSKALVRKAMVKRFPGLGFENQKKLLATRYCQDIYRNQ
                     GPRIWEELGSARQLAELGLVDLDRFHGILPACLRSNSQTALHRVWHGAFVEAWVRIYQ
                     "
     gene            23703..23870
                     /locus_tag="VFA54_10870"
     CDS             23703..23870
                     /locus_tag="VFA54_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51355.1"
                     /translation="MVQQRGEATVCNKGKWASPELLFDGELSGLVQQGGGKFSATGGD
                     PGEGKKQQSTT"
     gene            24046..24843
                     /locus_tag="VFA54_10875"
     CDS             24046..24843
                     /locus_tag="VFA54_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51356.1"
                     /translation="MAVGDEYYLKIHRVGAFRIERGYSAAIGYPEPGVDLSSFQAAFF
                     HGVAPWLLQRQDWDCLHASAVLIPAGVVIFCGPSGCGKSTLARAWSERGAAIYADDAA
                     PFLVRDGVAMSARIPQRLRLREPAASHFPIERHDPFSASSASRELTYDLEPTLRPVSS
                     IYWPEPAAGGMRCAGPVIERVPATEAFSLLLSEAHCMSLRDPACNRKMVRNYLSLVRS
                     VPVFRLRYPLHLDLLPAVLAELERCEKASVRDGRARASSRPAGCMEG"
     gene            25010..26458
                     /locus_tag="VFA54_10880"
     CDS             25010..26458
                     /locus_tag="VFA54_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51357.1"
                     /translation="MTALLGVVSAGLLIFSQTAAFYPDEGFHLLAAQLILAGKKPYLD
                     FFYPQAPLYACFNAGWMWLFGDTWRAAHVFSALMTAAGVALAACFVFTRMRGTRWEFA
                     AGAAAGVFIGLHLLVMRFGTIGQPYGLCLFLIVGAFWLTAESAIRPGKLWPLGAGFSA
                     GAAAGASLLSAPAPVILLVWTVARRRARRLESCLLFLGGAAAAWLPVLWLAAEAPRPA
                     LFNLFQYHLFHRRAGYRATHNVALSDLRVLSGWLHSPQSFWIFLLAVLGLLWVSAERE
                     WDEHRRSEMYLCASLVAGFAAFLALTHPAFPQYFVLLIPFLGILASVGVYAVSSRLSP
                     RARPWTAPLAALAVFLLGLGKTAYSQRDALQNHWKEIEDIAREIDRVTPEDGKVCAGE
                     AILFAARRLPVSGLENSYSHYLQLTPELAASLHVAGPAEVDKWIAGGVFDTILTWPPD
                     DPAPARFDLARRYVRRTKVHDCEIFSGKIAAQ"
     gene            26472..27983
                     /locus_tag="VFA54_10885"
     CDS             26472..27983
                     /locus_tag="VFA54_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51358.1"
                     /translation="MLLSLVVICLTAGLLIFSQTLAYYGDEAFHLLASQLILLGKTPY
                     VDFFYPQAPLYAYWTAACMRVFGDSWRTAHMFSALLTSAAIALLPGFIRAVIPEARWR
                     IPIAITAVMLAGLNLLLIRFGTIGQAYGLCLFLIAAAFRLAVKAADSSARWLPLWAGI
                     CAGAAAASSLLCAPVAPILLAWLIRYGSHANRLKTAARFAGGAAIPFLPLLWISLGAP
                     RQTLFNVIEYHLLYRKVGYPHYNITLADLSVLSGWLSSPQGLLLAFLSGLGVLSTAAS
                     GEWSEAGRRNLYLCIWLGAGLGAYLATPHPTFPQYFVLLIPFLSILASVGVYAIGSRI
                     CPLIRPAQLALAAIGLFALGLAKSAYRQRNELYSLWPEIEQIGRVVDQVTPRNGLICA
                     DEAIYVAAHRAPPPGLEHSDAHKLRLSPPLASLLRVIPFAERDQWISEGRFDTVVMWF
                     PSMERVNMLGLPRLYARSTKVGDCLIYSGRTAASGRLPARASAENSASLNAKR"
     gene            28060..28578
                     /locus_tag="VFA54_10890"
     CDS             28060..28578
                     /locus_tag="VFA54_10890"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZS51359.1"
                     /translation="MRSASGSAATVRKTGGDYVVDPAVRRAKWVEVSAATVALMETRL
                     LSLKPQLEEHFHLKLAGCQRPQFLAYREGDFYAPHRDNSKDPQAAEIPKQRRVSVAIF
                     LNSESGEPADGSYVGGSLTFYGLLHDPQKGAVGLPLVGETGLLIAFQSSLVHAVAPVT
                     YGERYTIAGWFF"
     gene            28641..>29756
                     /locus_tag="VFA54_10895"
     CDS             28641..>29756
                     /locus_tag="VFA54_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51360.1"
                     /translation="MKNSSLRAISLVAALAAVSALGCAGRAAVRMFLSERLIETGSEQ
                     NLRKALRLAPLNATAWSQFGALLENRGDTESAAKTLQHAVDLDRYDSGALINLGLHWE
                     IGGEPQRAEECLREAVRVNGAFYPRWVLANFYLRQGAAEAFWKAMQDAMSVRRADLTS
                     VFQLYWRAYDDADEILQKGVLDRPDINRQYCDFLLSTGRVPAAAGVWKRIAPQLQARD
                     LQLGLRYVEALLAQRQIEDGVAVWNRLCEAKLLPYQGLDPARGRLLTNGNFAASPSAR
                     GFDWKVGENEGINTALLASSAGPELRIRFSGAHAEAVDLLSQLAPAAAGQVYQLTFRY
                     STAALPPDTGLYWSIQDALSGERLGEAPLAAAEEAWKQ"
BASE COUNT         5563 a         8912 c         9184 g         6097 t
ORIGIN      
        1 gagatccagc gaaccaaaaa agcggttcag ctcgcccgtg gcaatccggc gatgcgcggc
       61 gtgatagact cgctgaattg caggaaacaa gcgctccgtg cccgagccgg taagggccga
      121 gacgaactcc acgggggcat aatccagaaa tttcagcttt tctcgcagac gcgccgtgaa
      181 ctcctgccgg cttgtctgcg tccgggcaag gtcccatttg ttcaccacga tgacaattgg
      241 cttgccgctc tcctgagcgt agccggcgat cgtagcgtcc agggccgtga ccccttcgac
      301 cccgtcgatc agcaatagga ccacctcagc cagccggatg tgccgccttg ccatcactac
      361 gctgagcttc tcggccatga gcttcgtctt gcctttacgc cggattcccg cggtgtcgac
      421 gaagttgtac gccacctcgt cgcgccttac cagcgcctcc actgcgtcgc gggtggtccc
      481 cggcgtaggg gaaacaatgg ctctctccgc tcccgccagc cggttcagca gggtggattt
      541 tccggcgttc ggcttgccga tgatagccac ctgaacgggc ttcttaaaag accccagccc
      601 cgttgtcttc ccgacttcgg aagcatcggc ggcagggaaa tgttctgtga caatatctag
      661 cagttcgtcg acgcctttat tgtgttcagc ggaaacaggg actacgcgct cgatgccgag
      721 ggagtagaaa tcgccgatgc cgctttcgag cgacaccgca tcgaccttat tgacggcgag
      781 cgtgagcggc ttgccggtct gtagcagcaa acgcgccagt tcccgatccg ctcctgtaat
      841 ttcggttcgt ccgtcgatga cgaagataat gtgccaggct tgttcgatgg ctatccgcgc
      901 ttgttttaaa atctgaaccg gtatttcttc ctcttcaccg acgagaatgc ccccggtatc
      961 gatcagttcg aaagggcgtc cccgccattc ggccggcgag tggatccggt cgcgggtgat
     1021 gccgggctca ttgcccacga tcgcccgccg aacacctgta attcggttga aaagagtcga
     1081 ttttccgacg ttcggtctgc cgacgatgac gacagtgggc cgctcgcttc gttccatcag
     1141 ccttcatggt agcttaggga agcggcgcgc aatggacggc tgcgccggca gcttgcacga
     1201 gaaatggcgc tctattatcc gctttttcta aacatccagg ggcgaacggc ggtggtggtg
     1261 ggcggcggcc gcgtggctgc ccgcaaaatc gctggtttgc tggaggcggg cgccagagtg
     1321 cgggtggttg cgccggagat ttcgcgccag gtggctgcgc acgaaatcga gcgccggcct
     1381 taccggacgg gcgatctgaa gggcgctgtg ctggcttttg ccgccaccga cagccgcgac
     1441 gtcaatcatg ctgtggccgt cgaagcggcg cgccggcgca ttcccgtgaa tgtggcggat
     1501 tcggcggcgg agtccacctt tatcgtgccc gcgcgcatca cgcgcggcga aatgcaaatc
     1561 gcgatttcga ccggcggccg caatccccgc ctggccggac aaatgcgccg gcggctggaa
     1621 gccgaactga ggtgggccgc aaagtgaggc gaggatgagg cttcgcacgg ccgagggcaa
     1681 gctcaagatc gagcacagca tcatcgaggg tctgcgcccc gctctggaac gcctgctgcg
     1741 ccacaacccg gaaatcaagt cggtgatccc gggagtgatc cgcccggtgc gcgacgcgcg
     1801 cggggccatc cggatccgga tcacgacccc tactcaaacg ggctggaagg ttattgcgtt
     1861 cgccgccggc gcccggcagg aggtttttgt aaataccggc ttgacccgcg agcaactgga
     1921 agccgctctg gcctcggcgc tgggcgaatc agcgtaagaa ctcggtaggg gcagccggat
     1981 tcgattcgca aaaatttgtg cggcggcagt tcccgaggcc gggtaccgag cgtaatttcg
     2041 aacgagccgc ttcagcggca gatcacctcc tcgccgcgcc attcagcttc gaccaagggt
     2101 ttttccgaac ggcgtataaa tgcgcaatag gcggccgtaa ttttgctgcg ggcagggatc
     2161 cagcggtacc cgggtgttcc aaacagagaa ccccggtcaa tcatgcggcg acgcggctcc
     2221 ggaaagggcg agaccccaaa ctccattccc cgcgtgagtg tacgcccgtt ccagggctta
     2281 tgagtgcggc agtgattctc ttcccaaatg cctaaccaag ggaaatctgc ccgccgccac
     2341 acgtatccga agagcacttg cgcgtccgga tcatacgctg taaaccacgc ctgctcgcgc
     2401 gccggatcca tgagatgcgt cgtgaagccg gccgacacgg gaacatcggg gatctggcgg
     2461 agatcgaggc agccgccatc gtttgcagga cacaagggcc agtcgaactc ggcgccaacg
     2521 cgcatgcgcc ctttcccttg ggcgaactcc gtttcgtagg ttttcgaacg cgtcgcggat
     2581 gcagcgaaaa tcgtcctgcc gcgggagaga aacggcggcc cgagagtcac atgttcggtc
     2641 cagccgacag cccggtcggc cgccgagagg ttctccgccg tctcggtaaa aagcacaact
     2701 cccccggatc ccaggcgcag ccgcctctcg aaacgcattt gcgcaacagg aaggagcgcc
     2761 ttgcaaagca attccgattc gctgattgtt tcgatttccc agcggacaac gggtgcttcg
     2821 ccgtgcacgc ccaagccggc cgccgcttcc tcgtcggaag gggccccgaa gtaatcgaag
     2881 cagagattgt ggcccaaaat gccggaaagc aggcggctct cccggtcaat gccatactcc
     2941 ggcgttcggc gagggtccca gtcgcttggc tcgatgctcg gccagggcgg gacccaaagc
     3001 gggttgaccc cgttctctag taaacagatc tcggcgacat gcccgccccc atacacgact
     3061 gagacgcgaa tctccctgtt gtggagctgc acggcacggc ggccgtgaaa ggtagcctcc
     3121 atcctgtctt ccccccttgc cctccgaaag caatgattgt actgtaaagc gttatggcat
     3181 cccgcagtgt aaacaaagtg attctgttgg gccaccttgg acgcgatgcc gaaatcaagt
     3241 acacgcccaa cggagcgtcc cgctgcacct tcacgctggc cacctcccgc cgatggaaag
     3301 accagcagac tggcgagtgg aaagaagaaa ccgactggca caacgtggtg ctctggcggt
     3361 cggagaacgt ggctcctcac ctgcaaaaag gcaagcaggt gtacgtcgag ggtcgcttgc
     3421 agaccagaag ctgggaagat aaggatggca acaagcgcta caccacggaa attatcgcgg
     3481 acaatctcat tctgctcggc gggcggggag gagaggcgat cggagcaggc gacgaagatc
     3541 ccgcttccgc ttcggcctcg ggtccgcgca gcgcaggggc tgggcgtggc tttacagccg
     3601 ccccgcaatc cgacatggcc agccaggaaa tctccgacga cgacgtacct ttctaggcgg
     3661 cttttgcatc gtgccccgag tgaacgcgcc gggagactgc ccgttagtct cgctctttga
     3721 gatacagctt tgtttctggc atttgccacg tcaaacgcct caccaagggg tgaaagccgg
     3781 cggaacgcct cgcttctgcg caaggacctg tttgcgacaa gcgcgttcgg attgtacaat
     3841 tgccgttccg ccggacgcca tcagcttgcc ttggcccgcg ccgtggagtt gacgattcat
     3901 gcgccgcggc cgaatccggg gatataatct cctatcctga ttttctttgc gggaggtagc
     3961 tgtgacgcgc aaagtattct gcctatcctt gacggcggtc gctgccggct tgctggcgca
     4021 gcagcaggcc ccgaaggccg ctgccccgtc cgggctgccc aagatccagg cgctgattat
     4081 caccgggcaa aacggccacg actggcgggc gaccactccg gtgatgcgga agattctgga
     4141 agacaccggc cggtttgaag tgcgagtcac cgaagagttt cgcggcgccg gtcccgagac
     4201 cctgtctccg tatgaactgg tgatcctcaa ttactacgag cgccgaaggc ccgagctgcg
     4261 ctggggagag cgcgccgata acgcgttgct caattttgtt cgttcaggca aggggctggt
     4321 gatgtaccac ttctcgatgg cggcttttga cggctgggaa gaatatgaga agctctccgg
     4381 cgcgaactgg cggcccaata acggccacca ctccgcccgc cacaatttca ccgtggacat
     4441 cagagacgcc gaccacccga ttacgcgcgg catgaagagc tcctttccgg aaaccagtga
     4501 tgagctgtat gccaatttga aatggcaacc gcccaataca tttcacgtgc tggctaccgc
     4561 ctgggatgat cattcactgt atcagggcaa agcccggcag cccattcccg gacccggaaa
     4621 agacgaaccc atgctctgga ccgtaaacta tgggcaaggg cgcgtcttcg ccacggcgct
     4681 gggccatgat cccgaggcga tgaaaagtca cggattcatc acgacctttc agcgcggttc
     4741 ggaatgggcg gcgaccggca acgtcaccat accggttcct ccagagctgg cgaaataata
     4801 agcggcggca ggccgctctc tgttccgggg cgcaaggcgc gcttgcctgt gggtgagacg
     4861 gcgtggccgc gagtgaaaaa gggtcaaaac gggaaagaac gaacctgggg ctgaaagcgt
     4921 ccgcacaggt tacactagac aaaatggccg ataattttct ggtacccgaa accaaaatcg
     4981 aggccaatgg cgccggccca gcgctggcgc tggggccctc cgcaggcagg accttgctgt
     5041 tgacgctcgg catcaccaag atcctcgagc agcagagcct tgacgtctcg atttggggat
     5101 ctgtggacgg cagcgattgg ggcccaaaac cgctgctcag cttcccccaa aagttctatg
     5161 ttggctccta tacgctgctg cttgacttga gcgacaagcc tgaaatccag ttcttacagg
     5221 ctcgctggac ggtgaaccgc tggggcgtcg gttcaaccaa gccgatgttt ctcatctata
     5281 tttttgtgga agtagcgtcc ggcgtgaaga ccgcatagtc tgttccggcg cggctgcggt
     5341 cgaatgctgc gttgccagcc cgcccttcac cgcttctatt ccggttcgcg cttcttgccc
     5401 ctgcgtaacg cctggcaaag tcgcagcggc tggcattgtg cgattcagcg aacaagccgc
     5461 ccatacccta ttttgctctg gatttcttca tccttcaata aatgtccgat tttcaagagc
     5521 ttacctcttc cttccgggat tgaaatgcgg taaaattaag gtttaatcta cacccgggag
     5581 atctctttgg acgacatcga aaagccaccg cagggtccca cgcaattgcc cctgccgccc
     5641 gatggcggcg acaagagtct ggttccgatc gacatcgaag acgagatgcg caagtcgtat
     5701 ctcgattacg cgatgagcgt gatcatcggg cgagccctgc cggacatccg cgacggactg
     5761 aagcccgtgc agcggcgcat tctctacggc atgtacgagt cgggattgct gcccaaccgt
     5821 ccttaccgaa aatgcgccaa cacggtaggc tacgtgctcg ggaattatca tccgcacggc
     5881 gacgcaccgg tctatgaagc gctggtgcgg ctggcccaac cgttttccat gcgctatccg
     5941 ttggtggacg gccaaggcaa tttcggttcc gtggacggcg acccgcccgc ggccatgcgg
     6001 tacaccgagg cccggctggc gcgtatcgcc gtcacgctgc tcgacgatat cgataaggaa
     6061 accgttgatt tccggcctaa ttacgatgac cgcacagtcg agccggagta cctgccggcg
     6121 ctcattccga atctgctgat caacggcgcg cagggcattg ccgtcggtat ggccaccaac
     6181 atcccgcccc acaatctcgg agaaatcatt gaagcggcca ttttgatggt gcagaacccc
     6241 aaagtcggcc tggacaagat cctggagaag gttcccggtc ccgattttcc caccgggggg
     6301 ttcatcctgg gccgccaagg tattatagat gcctatacga aggggcgggg ccacctgaga
     6361 ctgcgcgctc gggcagccat cgagcgcgtc ggcaaggaca aggagcagat cgtcgttacc
     6421 gagatccctt accaggtaaa taaagcccgg cttcaggagc atgccgcaca actggtgaac
     6481 gaaaaaaaga tcgagggcat tgccgatatt cgcgacgaaa gcgaccgcga aggcatgcgc
     6541 atcgtcttcg agctgaagcg gggagaacag ccggaggtgg tcctgaacaa cttgtacaag
     6601 cagacgcaga tgcaaaccgg cttcggcatc atcatgctgg ccattgtgaa cggccagccg
     6661 cgcgaagtcg gtctggccga ggccctgcgt ttgttcatcg atcaccgcat cgacatcgtg
     6721 cgccgccgca cggattacct gctgcgcaaa gcgcgcgagc gcgagcacgt cttgctcggc
     6781 ttccggaagg cgctcgaaaa cctggatgcc gtcatcaagc tgattcgggc atccagatcg
     6841 ccggccgaag cgcgcacggg tttgatggag cgcttcgagt tcaccgaaag acaggcgcag
     6901 gccatcatcg agctgcaatt gcagcgtctc accgggatgg agcgccaaaa gatcctcgac
     6961 gagctggccg aaattcaaaa aaggattcag gaatacctcg agatcctggg ctcggataag
     7021 gtcctgcgca atgtgatcgt aaaagagctg cgcgaggtgc aaaaggaata cgccgacccg
     7081 cgccgcacgg agatcatcga tgacaccggc gaaattcagc tagaggacct gatcgccgat
     7141 gaagatgtgg caatcacggt gactcacaac ggctacatga agcggactgc cgtggacacc
     7201 taccgcaaac agtctcgcgg cggcaagggg cgcatcggca tgggcacccg cacggaggat
     7261 tttgtcgagc atctactcat cgcctcgacg cacagctaca tcctgatctt taccaacctg
     7321 ggccgcgttt actggctcaa agtgtacgag attccagacg tcggcgcagc cggcaagggc
     7381 aaaaacgtgg ccaatctggt caatctccag cccaacgaaa cggtcaaggc gttcttgccg
     7441 gtgaagcagt ttaccccggg cgtctacgtc gtgatggtga ccaagaaggc gatcatcaag
     7501 aaatgcgagc tgacggtttt cgataacccc acctcacgag gcatcatcgc catgtcgctt
     7561 gaggaaggcg acgaactgat cgcggccaag ctgaccgatg ggtcgcagca gatctttctc
     7621 ggcacccaca acggcaaagc catccgtttt cccgaaagca aggtgcggcc gatgggccgg
     7681 ccggcttacg gcgtgcgggc gatgacgctc gacccgggcg attttctggt cggcatggag
     7741 gccgtgggcc tggaaggtct tatcctgacc gtctcggaaa atggctacgg aaagcggacg
     7801 gccctcgaag agtaccggat cacgtcgcgg ggcggcaaag gcgtcatcaa tatgaaaacc
     7861 acggagcgga atggcaaggt cgtcgccatt ctcaatgtga aggagagcag caatctcttc
     7921 atcatcacca aagacggaaa gattctgcgg ctcgaggcgt ctgagatccg cgaggccggc
     7981 cgttccaccc agggtgtgcg cctcgtgcgg ctggaagaag gtgaccgcgt cgccgctgcg
     8041 tcggtgattc ccgaggaaga atcgaacggc gaggggccta acggccaggg cgccctgcta
     8101 acgcaataga gaatcgcccc ggcgccgcga gcactgggat gcatattgca aaatcgcggc
     8161 agaacgcaag gccagccgtt ccggcttggc caggcctggg tctccgggag tggcttccct
     8221 tcctggctcc gccgtggaca gacggatcgg cgcccgtttt cccgttggaa gatccaattc
     8281 tcgactctgc cattcgattt tgatgctctt tgcgttcctg acttcaacgc cgatggacct
     8341 gctgcgcggc agcggcacct ttgcgggcat tcaggggctg gctgagggtc ttcgagggct
     8401 cggccacacc gtcagggtcc tcactcccaa cttcccctgc ttccactaca cgacccggcg
     8461 gctgatttac aaccgccgcc tcgccgcccg cgtcgatgcg ctccggcccg acatcgtgat
     8521 cggcttcgac atggatggat accgctatgc ccggcgctcg ccgcgcgcgc cttttgtcgc
     8581 ttccatcaaa ggggtgatcg ccgatgagtt gaccaacgag agcggcgcga cgcgctggat
     8641 gatgacggtg caggccaagc gcgagaaaat caacgtgctc cacgcggacc tggtgctggc
     8701 caccagccag tattcggccg ccaaaatcgc ggaacactac ggaatcgaaa aattcaaagt
     8761 ccatatcgtg ccggagctga ttgacctgga gggatggcgg gcgcgtttcg cggcccaccc
     8821 ggccgccgcc cccgagcgct ttacggtcct ttcggtgtgc cgtttttacc ctcgcaagcg
     8881 cctggaggat ctgatcgccg ccgccgcgct cctgccggga acggaggtgc gcattgccgg
     8941 caacggtccg gaagcaaaga ggctccatcg gcttggcccc catatttcct ggctcggcga
     9001 cgtcaccgcc ggccagctcg ccgaagaata taaccgctgt cacgtcttct gcctgccgag
     9061 cgtccaagag gggtttggca tcgtttacct ggaagccatg gcggccggca agccgatcgt
     9121 ggccgcccgg gcggctgccg tgccggaggt cgttcgcgac ggcgtagagg gcatcctggt
     9181 cgagccgcga gatcccgctg ccctggccgc ggcgctggct cgtctgcggg acgacgctgg
     9241 tctgagaaat acgttgtccc ggaatgctac cgcgcgcgcc gctgattttc aaagagaaaa
     9301 agtttgccgc aggtttctgg acgcggttga gaaattgcgg cggtcaccgc cgccctgctc
     9361 gcgccaggct acgacggctc gacatcagtg agccgcgccg gccggcgcag tggtggagat
     9421 tccccgcaag cagcgttttc gccgaaaaca gcaggcggcg gaccggtccg ttatactggc
     9481 attccgtggc aaactgcaca gccagcgcga ttcgacggcc ggtgaagctt tgcctgccgt
     9541 ttgcagcctt cctcctgttg gaagcttgca gcctgatgcc cgctcggccc gcccgcgccc
     9601 cgcagcgctg ccgaaacctg atccgcaatg ccaattggga gcggcaaggc gagggatggc
     9661 gtctgcccgc gttcgccaaa ctcgaacagc cgccttcttc gggaggcgtc gcttccatcg
     9721 ttgtagaagg cgccgccgat ggcgcgatcg cccaaatcat tccccgtccg ccggaacttt
     9781 ccggcctcgg agtggtcgcc gtgggttatg ccagagtcga gttgtctcat accctcgata
     9841 gcacggatga cgatataacc tcccgcctgt gggaaggcag cgtgaatctg gtgaacgggt
     9901 atagccgcaa cggggaagac ttcgccagcc cgtatgtccg aatccgcttc acgggccgtg
     9961 aaatcgcctc gggctgttgg aaacggtttg taaccggtgt aattccagcc gggcgggcgc
    10021 gcctgctata tccgcatttt gctttttggg gggcgcggct tgcgcccgga gtgaagctcc
    10081 acctggcggc gctatctctt gttgaagcgc ccgcagacga caatggcgag cctgagcggt
    10141 gggtggattg cgcgaacctt cccgcgcctt ctcccgtgct ggcagcgccg acgcagcaac
    10201 agtgggccga atcgctggct cctaacggaa gttttgactc cttccatatg caaacccggc
    10261 ctctcgcggc cgcccactcc ttacgcgaac tggtgctgga agcgcgatat caccagggat
    10321 ctttgatcgc cgaccggtat ttgatttcgg ttacgcagga cggctccgac ccccgtttga
    10381 gccccacaag tcacatcgtt acggccctgg ccaggcgtgg cgtggaagat ttcgaggccg
    10441 tcgtgaaggt ccgcgcggac gcccagcctc tccgcatcgc gatcgccggc gccgccgaag
    10501 tcaaagacgg attacgcgcc cagccggcaa tcttgccgct cgaatggcaa agacctcgcc
    10561 tctgattgca aatgccgtcc gcggaacgcg aggatcgtag gttcaggtgg attgccggat
    10621 tttcgtatcc gctcctgctt tccgtatacc tcatttattg gagccacgcc gactatttcg
    10681 tggatgattg gttcctcatc aagcatttcc gccatgcggc cgggtggaag ggagtcgccg
    10741 gcttcgccgc ggcggccgcc gaaaaccgcg tctatcaggt cttccggatg caatggttga
    10801 gcatccttta cgggtttctg attaccgctc tgggcggcta ttctccccgc ttcaactttg
    10861 cggcgctgct tctgctccat gcggcttccg cctggctgtt gtcgcaagcc gtgcgccggg
    10921 cgggcttcga gaggggactc gcgtttctcg ccggagtttt ctatcttctc gcgccgatgg
    10981 cgcatttcgg gctcctcacg tatcttgcaa atccgttttt tgtgttgtcg acattctggg
    11041 ttttactctt gctctggttc tcttcgggtg cgcggcggcc gggaatgggg gagcttgctt
    11101 ttgcctccgc ctgcgccgtg gcggccctgt tcagcggcga acaggttttc ttgctggtgt
    11161 gggcgatcgt tccgctcgcc gcgttgtttt tccgaaagaa gccgataccg gcggcctggc
    11221 ccgccgcggg agcaatctgg gcggccgcga gcgcggcgct ggcggtctat ctactctgga
    11281 tcaaccggct cccggtgcgg cagaccggca tccaccgacg ctatgaatgg acttcggccc
    11341 ggttcgcgag caacctgctg caaatcgggg gcgagctgcg gcaactgagc ggagtcacct
    11401 ccgactcgct gttccatttt tcgctgcaca agacggagga cgccctcgcg ctggcgggcg
    11461 ccctcctggt gactgcgctc gtttggaagt ggcgcggcgg agatcgggaa aacggccggc
    11521 tccggcgcct atttggattc gcggtcggcg gagcagcgct cgcatatgct ccggtgatgt
    11581 ggattgccgg cggctttccg cgctttcggt atcattatgc tccggcgccc tatctgggtc
    11641 ttgccgccgc tgtagcggtt tgggcgtgtg ggcggagccg gtggacgcgc tggagtcccg
    11701 ccgcgctagc cggcctcgct acgggctttt tgatcttcaa tgcggccgca gatctgcggc
    11761 agagctggat tccggaatcg gaacagcaac acaatctcga agtctatatg cgtcaggtga
    11821 aaaatatagc gcctggagac atcttcgttg tctcgggcac gccgccccag attggaaccg
    11881 ccccgcactt cagcatgcat tctacgattt cggccagcgc gtttgtagag ctcgtgacag
    11941 gcctggagcc gctcgaagcg tatctcgatt tttggaatct gcatgggcgg ctggtgcgcg
    12001 atcccgatga tctggagcac tttctttccg agtctgacct agcgcgcacg cacgtcctga
    12061 tgtgctccta cgacggaaga ttttccagtc tggattggat cgcgatcgag atcgggaccg
    12121 acagcttccg gctgctgccg ctgaagggag tctccgattc gggcaacgcc cgccggcaga
    12181 tctattcccg agagcagctc gctcttgcac agtggccggt ctatttcccg aaaattgtga
    12241 ggccccgagt cgcggcccga taagacggcg gcccgcgctg tcttactgtt tgcgccgatt
    12301 gccgattgga tccgtcggcc agcggatgga aaggcgcgcc ggctgacccc aaaaatcgcc
    12361 ctgcccggcg atgtatgtgc ttggagaagc acgttcggtc cgcatgggat aaaattttct
    12421 actgaatttc ccgcgtgcac ttggattcca aagcgctctc gttgaaacag ccggaggtcg
    12481 cagttggcgc tggccgactg cgcgtgctgg accgcctcgc gcccgcgctg ctggttcccc
    12541 tgatcatttt taacctgggc tacatgtacg aaggccctaa ttatgctttg ctctcgccat
    12601 ggatcattcg cttgtcgcgg ccggaactgc tggcccgcga ctggtttgcc aacacgattc
    12661 cgcaccatgc gaattttgtc cggttcctcg cgtggtcgtc ggcggctttg ccgctgccgg
    12721 cagtcctgct ctcactgcac gtggctgcac tttttcttct gctttggacc gcctatggat
    12781 tcgccgcgcg gcttttcggc gataaaagga ttttttatgc agcagtgttc ctgcttttgc
    12841 gctggggaac ggaaggtctt ggaggcaaca gcctttgggc cgactacctg gtgccgcaca
    12901 acgcggcggc gccggtctgt ctgctggcgt tctacttgtc cctgtcgggc cggccgctct
    12961 ccgccgcgtt ggccgcggcc gcagcaactt ggattcacat ccagctcggc gtcttaacca
    13021 tggctgtttt gggcggggaa atgctagtaa gctgtgtctg gggtgggggg cggagccctg
    13081 cccggagccc ggtccggaaa ggcgccagcc tcgggccaat gttcacggta ggcgggatct
    13141 acgccgcggc agtcgccccg acttttctga agcaatgggg cctttacgtg atgaccgctt
    13201 cgccactcac ggccaggcaa tttttagatc tccacgcaat tttgcgccag ccccatcacc
    13261 tgatcccatc gacttggccg ttggcggatt acgcccggtt ctttctcatt ctggggatgg
    13321 gcggcctggc cgtctcctgg cgaaatgctc agcaccgtaa aattgtggtc tggagcttta
    13381 tcattttggc gttttgcgta gcgggcagtg tttttgtgga gatcattccc atcaagctgg
    13441 ttatcaaaat gcagttgttc cgtatgacca tcttcttgaa attttttgcc gttttgttcg
    13501 ctgcacaatt tttaatcaga acactcgacc tgggccggtg ggaagagaaa tgctgcgccc
    13561 tggccatact ggcaattcaa aatttcttcg tagctggcgc gtgcgccgtg ctcatcccca
    13621 ctttgcgtaa gaaccgccgt tgggcgtgga ccttaagcat cgcggcggcg ggatgtctgg
    13681 cgctcagtgg gatcgcgggt gcgggcgttg gcgggtggct tcagcatgct cttcgtatgg
    13741 cgccccgagg aatcgaaatc ggcctgatta cactgggttt cctcggggcg atcgtctggc
    13801 gaaacggccg gctcttaccg gcgggactga tgatcctggc agcggcgctc cccctcgcat
    13861 ccggcattcc ctctttccat tatgatcatc ctccgatcga cgactggtac cgcttttgcc
    13921 gccaggtaag agccgcaaca ccagcggatg ccgtctttat cacgccgccc gtaatgggag
    13981 gctttcaaat gttcgccgag cgggcggagg tggcggactt caagtcaacc ccttccatcg
    14041 agcatgatct ggtggagtgg aagcatcgga tggacgatct ggcgggcggc gccggcttgc
    14101 attgcagcgg atgggcggag tgcagcgctg ctctgacctc cggctacgcc catttgacgc
    14161 gcagaaattt tatggtgtta gcccagaaat atcgggcaga atatgtagtc accccccgcc
    14221 cggagccgtg gctcggcttt tccgaagtgc ttcgcgtcgg agatttcgtg ttatatcggc
    14281 tgccggcata gcgccgttct ggtagactgt cgccagctag ctccggcggc cgggagcccg
    14341 tcgagacggg ttgcattcac gcccgccgga agattggttt cgtggaagga gatcgcatgc
    14401 tcagtcgaag acaattcctc cggttgtgct cggcgacctg cgccacctcg gccgctggag
    14461 tcgcagcagc caagcctcgg tttcgaatag gcatcaccac taacacccgc aatggcggca
    14521 gttgggataa agacatgctg ctctcgtttc gcgaggcagc cgaagccggc tatcacaacg
    14581 tggaaacatt ttacggctac atccggccgt ggtgggacaa gccgcaggaa ttaaaaagtc
    14641 agatggaccg tctgaagctc gggtttgtaa cgatttcgaa cggcgggcct atgaagatgc
    14701 agttcgaaaa cccctctgtt gcacagcaac tgattgacga gcacgtccaa ctggcgctgt
    14761 ggagtaaaaa gtggtttggc tgcgatcatc tgaaagccaa caccggaggc cgccgccccg
    14821 agggtacgac gtccgatgac ctggcccaaa tggccaggac catgaatgag atcggtaagc
    14881 gcctggcgga tcaaggtctt aagttcgggg tacacgcaca tctgtggacc caattgcaga
    14941 cgcgatccga aatcgatcac atcatggacg ccaccgatcc caggtatgta ttcctggtgc
    15001 tcgacaccgg gcacatcacg atggccggaa tggatccggt agaattggcc cgaacttacg
    15061 tcagccgcat tgtcgagttc cacctgaaag ataccaaacc cgaggacaaa ggcggccaca
    15121 aggggccgac tccgcagcgc gaagggtata cggcaatagg gaagcggatc ttcttcgagc
    15181 tcggaaaggg aggggtggat tttccgggac ttctatcggt gctgaaacag aacaactggg
    15241 acggctggct cacggtcgag ctggattcga ccgacacaac tcctaaagat agcgcgatga
    15301 tcagcaggcg gtatttggag aacgttctgc acctccaggt ctgaagcgac acggggcata
    15361 gaatcggaga acgctgggga agggagcagg aatacatact gggttcgtcc gcccagcctg
    15421 ttcgtcagcc tgggaactgg tggagaaaag gcctgctgga tggtctaata ctcctagcct
    15481 cgcggtacca ccagcaacgc ggatctccta tagaaatcaa gcacataacc agccagccac
    15541 gcgcatcgac ggcgagaaaa acccctcctt gtgaatatca gcgacttccg gtattcttat
    15601 ttagcgcttg tagtgttagc agaattacga tttttgactt agctgggtgg ggcgcggcga
    15661 gccctgagga tttgtttgca ggctatcgag tcttattcgg tcatccggcg caggcgagta
    15721 gataatcgtc tgaatttcca gcaacatcac ccgaaacatc ggccgagcca gaaagggcgg
    15781 gtttaccggc tctcgaggac cgaggtcgtc tgcaaccagg gctaagccca gtggataatg
    15841 ccggccttac aggaggagcg agctttgagg gaagaacggc ctgtcaggtc tggcggcgcc
    15901 ttcgggcagt tcgggttttt gagctggcac gtgataaatc gccgggtaga cggaaaagcc
    15961 gctgggttct tctgctgggt ccggaaactg gaggaagcgc gtcatcctgc gttgtaagtc
    16021 atttggctgc ttggagccgc cttcgcaggg tggatcggcc ggcaagcggt tccggggcgc
    16081 taggcgccgc gcttgtttat atgttattga atgaaaagca cttacagact gtgatgtaag
    16141 tgaaggggcg aagctctgtg caaacgatgg cagtttcaga ttccgtaacg caggtgctat
    16201 ccgacgttgg ccgcatggct cggcctacag cgccagtccc aggctggtac gccatctaca
    16261 cccggtctca cttcgaaaaa cgagtggctg ccgaccttac tgccaagcac atccagaact
    16321 acttgcctgt ggtggaacag gtacatcagt ggaaggaccg caaaaagctg atcgaagttc
    16381 ccgttttccc cagttacgtg ttcgcgcgct ttcgggattc gaataagacg cgcctggagg
    16441 tgctgcgggt agccggcgca gtccgaattc tgggtcacgg ggagagcatc gaaccgatac
    16501 cggattggga aatagaatcc attcgccggc tgctgcgagc caatgtgcgc tgcttcgcgc
    16561 acccgtttct tcgcgagggg gcttgggtcc gcgtgaagag aggagcgctg aaggatttgg
    16621 aaggcctttt agtgcgggtg aaaagcaaaa cccgccttgt gctttccatt tcgttgctat
    16681 cgcagtcggt ggcgacggag atcgatattt cagatgtcga ggtgatacgc cccggcagca
    16741 ggacgaatgg tgagggctgg cgatgagaag acgtttcatc gtccctgtat tactgctggt
    16801 tgcgccgttg ttcgggcaag atcgcggaaa ccgccgcgag caagagagcc aaccactgcc
    16861 gcagagcgag gcagccgagc cggcccttcc tggcgcggac tattcccgag gcgccggcgc
    16921 ggcaaacgcg gctgccgcgc cccgggccgg cgctctggct ttcagcaatt acaagatggg
    16981 accggaagac ctgctggccg tcactgtctt ggattctccc gagtttaccc gccaggtgcg
    17041 cgtgagcggc gatggaacga ttcggatgcc gcttctaaaa cgcgccattc cggcggccgg
    17101 gaaaaccagt gcggagttgg aacaggcgat ctcgcgggaa ttggtcgagg aagggatgct
    17161 tgtcgatcct tccgtttcgg tgaccgtgct cgagttcaac agcaaaccgg tcagcgtgtc
    17221 gggcgccgtg cgatcgccgg tggtctttca ggcggtgaag ccgctcagcc tggtcgaggc
    17281 catcgcgcgc gccagcggct tggcggaaaa tgccggctcc gacattctga tttccgtccc
    17341 ggcaaggaac ggccgtcccg cgcggaccgt taccgtgccg gtcaaggcct tgcaacaacc
    17401 ctctgatccg ggcgccaatt tcctgctgga ggggggtgaa gaggtcaaag tgacgccggc
    17461 ggggctggtt tacatcgtcg gaggcgtcaa taagccgggc gcggtcctga tgaatacgga
    17521 aggtcctttt actttgctga aagctttggc ccttagcggc gggacaacgc ccacggccgg
    17581 atcaaaggcc tttctgcttc ggaattccgc aaacggccaa aaaaaggaaa ttgcgttgaa
    17641 cctgaccaag ctaatgcggc gccaggagcc cgatctgccg ctcgaaaaca acgacgtggt
    17701 cttcatcccc gacagcacca tgagaaaaat cagccagacc ggcatgagct cggcggtcag
    17761 cgcggtggtg ttcgccatgg ggacgctaat tttacgatga gctcctatcc acggccgctg
    17821 cccggacccg gccaatatcc ggaagagtcg aattactatc cgatccagcc aaccggtgag
    17881 ccggcggcag tcccgctcaa ccactacctc tgggtcctca agcgtcataa atggcgcatt
    17941 ctcggattcg tatcagccgt ggtcgccgct gttctgctct attcccttca gttgacgccc
    18001 gtttatgaaa gcgtcgccac cctggagatc gataaccaag tccagatgtt tgaagtgggc
    18061 aattccggac tccaatttga caatcgcaac ttcgacgcgg ttgtagcgac gcagcttgaa
    18121 ctgatggatt ccccggcggt agcccagcaa gtgatccggg agttgcacct ggaccagaat
    18181 ccggatttca accctgcgct gcgcaacaaa gccacttccg acgctttggc cgccggactg
    18241 cccttgagcg gcgcgggcgc cggtgtgcct gacgctctgc ctggcctttc ggtgcggcgg
    18301 cgcccggata cctatttgat tgaggtacat taccgcagtc ccaatccgga gctggcgagc
    18361 gcgattgcga acagcgccgc tcaggctttc gtccaacaag gcttcaagag ccgttatcaa
    18421 aacgccgccg agctgtcgaa atggctcaat atgcagctcg acgaactccg cgccaagctg
    18481 gagcgcgccc agcagaagct ccaggcgttc gagaagcaat acaacgtggt caacccggaa
    18541 gatcgcacta acatcctgaa cctgcaattg cagaagcttc aggaagagct gaccaaagcc
    18601 caggccgagc ggctccgcaa ggaatccgag tatcagtccg tggaatccgg aaacctggaa
    18661 gcgctcgcca tttccgagca aggcgcgccg ctgatgaaaa tcgccgaccg gctggaaagc
    18721 cttgagaccg cattggcgga agccggctcg cagtacggtc ccaatcatcc catttacaag
    18781 cggcagcagg cgcagatcga gcgcgtgaaa gccctcctcg aggccaataa gcagaaagtg
    18841 ctgaagcgtc tgaaggccga ctacgagcag gcgctggctc gggaaaaggc tttatccgcg
    18901 gcagtggatc agcagaagca ggaagtggac cggctcagcg agcgcgcggt cgagtacggc
    18961 attctcaagc gggaagtgga ttcccaaacc aagttgtatg acgagctgct gaaaaagata
    19021 aacgaggcca cgatcaacgc gagcatcaag gctacgaatc tccggctggc ctccctggcc
    19081 acgccctcga aaactccggt gtttccccgg accaaagtgt atctgctgct ggctgcctta
    19141 ctgtctacca ccgtcgcggt cggcgtggct ctggcggccg attatctgga ccgcacgctt
    19201 cgctcggtcg agcaggtgga gcagtggttg agcgtgccgg tcctcgcgaa tctgccccgt
    19261 gtctccggcc aaaaaattcc ttccctgctg ctagggacgc ccgacgaaga aagcaattcg
    19321 accgcgctgg cgaaatcgag cattccactg accgaggctt tgaacatcct gcgcacgagc
    19381 gttttgctct cctcgccagg ccaggagcga agaatggttc tggtggccag cgcggcgccg
    19441 gcggaaggga aaagcacagt ggccaccgga ctggccctgg ccctggccca gcagttacag
    19501 aatggcgacc gcgtactgct ggtcgattcc gatctgcgaa ggccgacgat ccatacgatt
    19561 ttcggcctct ccaaccgcac cggcctctcc acagtgctcg aaggccagac cggcttcgcg
    19621 gaagccgtgc tgccgagcgg gatcgcgccg aacctcatgg tgcttaccgc cgggccggcg
    19681 ccgcgctact cgagtgaact gctcacgatg cacatggcga agctgctgga gaatctgcgg
    19741 ccggagtttc gctacatcgt gatcgattcc gcgccgctgc taatttgcgc cgacagcacg
    19801 attctaagca cgatggtgga cggcgtggta attgtggcga gagctggaga gacgccgcgg
    19861 gaagctgtgg cttccgcctt gcgccaactc cgccgcgtgc gcgccaatat tttgggcctc
    19921 gtgctgaacc aggttcgcct gccagataca cccggctacg gcggctatta cggcggctac
    19981 tacggaaatt accggcgcga cgaaaaagat gatgaggatg cctgagaccg ctggagcgct
    20041 ccggcttgcc ctgcctccgg cgtttgcccc ggccggccaa tcagcaggct tggattccct
    20101 cgcggccgcg ccaagaagag cggctctgct ggccgaggcc gagcgccttg gcgtgctgcc
    20161 ggcagtcgcg cggagattag tatcggaccg gcacccccgc gtgcgcgaga ttgccgccag
    20221 aaatctctgg ctggcgcggg aacaaggacg cgttctcaaa ttattggccg atgccggtgt
    20281 gcgggcgacc gcggtcaagg gcgtgaattt ggcgaggcgc ctgtatccgg acgcctcctg
    20341 gcgcgaagtg gacgacatcg atttgctggt ggatcgctcc gatgcagcca gagcctgcgc
    20401 cgttctctgc ggcagtggac tgacgcccaa ccaccgctgg accccggaag cgctgtcgcg
    20461 tcaattgtct cgccctctgt ttctggcccc cgaactcgtc tttacgtcgc cctctggcgc
    20521 gatggtcgag ctgcattggg actggccggg cgggcgcagc gcgccgctcg atcccttcgt
    20581 ccagcccgag cattacctgg tgtatctgtg ccgccacgcc gcgaagcatt tctggtcgtc
    20641 aatgaaatgg ttgtgcgacc ttcagctctt tgtgagccgg ttcggcgcca cagtggattg
    20701 ggaactgttt tggcgagtgg ccggagagaa cggagcggaa acaggctgtg cggccagtct
    20761 cgagttgtgc cgccgcgcgt tcggcggctt gccggcgcca gggttggccc agcggctgag
    20821 cagaaatgcg cgcaagctgg taaatcaggc ggaatcggtg atctcgaatc ctgctgcgga
    20881 tcggccgcat ccggtctgga cgcagatcca actcgcgccc tggcggtttc gtcccgcgct
    20941 ggtaaggaca tggatactcc ctcagccgca ggactggagc cgggcgcgag agcaacagca
    21001 gcccagctgg cagacggtga ggcggcggtt tggccatctg tggcagcggt gggcatttca
    21061 taaaatccgg aagctctcgg caaaagattg gggcattctc ctcgaggctt atttcacctt
    21121 gattgctgcc gaatgcgccc ggcgcctggt tccggcccgg tggctgctgc gcgcggcgaa
    21181 aggccggctc tcgagccgcg aagccggtcc ccaggcctgg actctcgagc agctccggcg
    21241 catcgcctgg ctcgtcgaag cggccgccaa ccgccagccg gtcacgatgc ggtgtctcac
    21301 tcggtccatc gctctttcgt ggatgcttgc ccggcgggga gttcggccgg cgctgcaaat
    21361 cggagttaaa aacgacagcg gcaaactcca ggcgcacgcc tgggtcgaat ggcagggcca
    21421 cgcattgcag gagcctgtga accaggcggg cgaatatgtc gcgctcgatc cgctggcttt
    21481 ccgataattc gtgaaaaacg caaaggagtg cttctctgtg gatttttccg cacgctacca
    21541 agccgacgct gaggtcactt tccagcggct cgacgaggcc actgtgatcg tccatctcgg
    21601 cacggggcgc attcatcata cgaatctgac cggctcccgc atctgggaac tgctggcgga
    21661 aggacgctcg ctggaagaca tctacaacac gctgcttgac gagtttgacg tgccgccgga
    21721 gcagttgcgc agggatttgg agagcttcgt ccaggaactg tcagcggaaa agatgatctg
    21781 caaccgggaa caaggcgcat gagcgctctc agcctcgcca ccaatgcctg gctcggcgtg
    21841 gtccggaagg aatctggccc gcgcggcgcc gtccccagga cggccgatgg ccggatcgag
    21901 ctcggagccg ctgtcttttc cctgcctgat cccaaagaag cgaataccac aagccgggcg
    21961 gtggagggcg gccggatttg cgtgttcacg ggcttcctat ttaatgccga ggatttgcgc
    22021 cgggagctcg gttgggatga ggagcggcct tgcgcttccc aagcggagat cgtgctggcc
    22081 ggctataaac attggggcga cggcgtgctc tcgaagctgg agggcgtctt tggcctggcg
    22141 atatgggacc aggccaagga gacgctgctt tgcgggcgcg atccgatggg cctacacccc
    22201 ttctattact cgctggccgg aggggaactg atcttctcct gggattctga gaccattcgc
    22261 ctgcacccca aggttccgag ggacctgaat cgcgtggtgc tcgccgagca cttgaattac
    22321 cgctggctga agcaggagga gacgttcttc caggccatcc gccggctccc gggcggctac
    22381 ggattgacat tcgcccgggg gcggatcgag gtgtttcgct attgggatcc cgcccctccc
    22441 ggccggcccg tgaactgggt gacgcgcgag gaattggagc aatttccagc tctgctccgt
    22501 aagtccgtcg aacgcacgat gaaatatggc cccatcggca tatttctgag cggtggattc
    22561 gactccatca gcatcgccgc ctgggcttcg gagatcgctc ccaaccgaaa ttgggccacg
    22621 ccgaaagcgt tttcgctggt gtttccggag ggcaaattct cggaggagcc cgtgcagaag
    22681 gccgtggccc aaaccctggg tttcccccag cgtttcgggt atatgacgga ctacgtcgcc
    22741 ggacggggcc tgctggggcg tctgttggat ctggcaggca cttgtcccgt gccaaccacg
    22801 gacgtcttca agccaccgta tctggatctg ctcaggatgg ccaaatcgga cggctgtgac
    22861 atagtgctga ccggagaggg tggcgacgag tggcttaccg ttacgccggc gtatgcggcc
    22921 gatctgattc gtaccggtaa tttcattgaa ttgactcgtc tgctcaaaac gattttgcac
    22981 tgctggaacc tgcctacgct gcccgcgata cgaaacgtcc tctggaccaa tgggctcagg
    23041 atgctgctgc gtgactgggg gtggcgaaat atgccaaagg cagccctgtg tcgaagaagg
    23101 cggctaattc ccaaggctat ccccgagtgg ctggcgcctg atcctgcact gaggcgggaa
    23161 ctggtggaac gtttcgaaaa ttcggacagg gaaatccgcg aggatacctt ctatttcatg
    23221 gggatgcgcg aatttctggc ccacccattg cttgcgatga cctttgaaga gcggttctat
    23281 cgagataggc tggccggcgt ccccacgcta cacccatatt gggaccttcc cctggttgat
    23341 ttcttgatgc gcacgccgcc ggaattgctg aacagcggcg ggcgctcgaa agccctggtg
    23401 cggaaggcta tggtcaagag gtttccgggc ttgggattcg agaaccagaa gaaactcctc
    23461 gcgacgcgct attgccagga catctatcgc aatcagggac cgcgcatctg ggaggaattg
    23521 ggttcggcgc gccaactggc ggaactgggc cttgtagacc tggacaggtt tcatggtata
    23581 ctgccagcct gcctgcggtc taactcccag accgcgcttc accgggtctg gcacggcgct
    23641 tttgtcgagg cttgggtaag aatttatcag taaaatggaa gtttcgaagg gaggaactga
    23701 gcatggtaca acaaagaggt gaggccacgg tttgcaacaa gggcaaatgg gcctcaccag
    23761 agctgttgtt tgatggcgaa ctaagcggcc ttgtgcagca gggcggcgga aagttcagcg
    23821 caacgggggg cgatccgggc gaaggcaaaa agcaacaaag cacaacctag ctttccagtt
    23881 gagtgacggc cagttgttgc atttcggctg gttgcgtcgc ttccatccac gttagtgatt
    23941 cggctctcta ttagaactgg gccggttcct tttccacccc atatagcgcc aaatggtcaa
    24001 gccgatatct ggcttgacgt tcgagggcag gaatacgggt cgggcttggc ggtgggcgac
    24061 gaatattacc tgaagatcca tcgtgttggt gctttccgga tcgagcgcgg ttactcggct
    24121 gcaatcggct acccggaacc gggtgtagat ctctcatcgt ttcaggccgc gttttttcat
    24181 ggagtggcgc cgtggctgct ccagaggcag gactgggact gcctgcacgc aagtgccgtt
    24241 ctgattcccg ccggcgtggt catattctgc gggccctcgg gctgcgggaa atctactctg
    24301 gcccgggctt ggagcgagcg cggcgcagcg atatatgccg atgacgcggc gccctttctg
    24361 gtgcgggatg gcgtggcgat gtccgcccgc atcccccaac gcttgcgtct gcgggagccg
    24421 gcagcctcgc atttcccgat tgagcggcat gacccgtttt cggcctccag cgccagccgc
    24481 gaactgacat atgatctcga gccgaccctg cgacccgttt cgagcatcta ctggccggag
    24541 cccgcagccg gcgggatgag atgcgccggt ccggttatcg agcgagtccc ggccacagag
    24601 gctttttctt tgctgctgag cgaagcgcac tgcatgtcat tgcgggaccc cgcctgcaac
    24661 cgcaaaatgg ttcgcaacta cctttctttg gtgcggagcg tgccggtttt ccggcttcgg
    24721 tatccgctgc atctggacct gcttccggcc gtcttagctg agctggaacg gtgtgagaag
    24781 gcctcggtcc gagacggccg cgcgcgcgca agttcgcggc cggcggggtg catggaaggc
    24841 tgaacggctc gctgattctc cggcgcctct gtgcttcgta gcttcccggc cgtggttcgt
    24901 accatccccc atgactgcgg cggttccttc tgatcgctat tcaagcgaag caggccaaga
    24961 gcagtctgcc ggggatgcac aaatgtgtcc gaggcctact gcggctctaa tgacagcgct
    25021 gcttggcgtt gtttccgcag gtttgctgat cttttcgcag acggctgcct tctacccaga
    25081 cgaaggcttt cacctgctgg ccgctcaatt gattcttgcc gggaagaagc cctatctgga
    25141 ttttttctat ccccaggctc cgctttatgc ctgcttcaac gccggatgga tgtggctttt
    25201 cggcgacaca tggcgcgccg cccatgtttt ttccgctctc atgacagccg ctggcgtcgc
    25261 actggccgcc tgctttgtgt tcaccaggat gcgcgggacg cgttgggagt ttgccgccgg
    25321 ggcggcggcc ggcgttttca ttgggctgca cctgctcgtg atgcgatttg gaaccatcgg
    25381 ccagccgtac gggctgtgtc tgttcctgat cgtcggtgca ttttggctta ccgcggaatc
    25441 agctatccgg cccggaaaat tatggccctt gggggccggc ttcagcgccg gcgccgcagc
    25501 cggcgcttcc cttttgtcgg cgccggcgcc agtcatcttg ctggtgtgga ccgttgcccg
    25561 ccggcgagcc cgccgcctcg agtcttgcct gctttttctg ggcggggcgg cagccgcctg
    25621 gctgcctgta ttatggctgg cggcggaagc gccccgcccg gctcttttta atcttttcca
    25681 atatcatctc ttccaccgcc gggcgggcta tagggcgact cacaacgttg ccttgtccga
    25741 cctgcgggtt ctatccggct ggctccatag cccgcaaagt ttttggatat ttctactcgc
    25801 tgtcctgggg ctgctctggg tttcggctga acgggaatgg gatgagcacc ggcgatccga
    25861 gatgtacttg tgcgcttccc tcgttgccgg attcgcagcc ttcctggcgt tgacgcatcc
    25921 cgcgtttccg caatattttg tattgctgat tccattttta ggcattctcg cttccgttgg
    25981 ggtgtatgcc gtcagctcgc gcttgtctcc gcgggcacgg ccctggacgg ctccgctggc
    26041 ggcgctggct gtgtttctcc tgggtctggg gaaaacagcg tattcgcagc gggacgctct
    26101 gcaaaaccac tggaaggaga tcgaagacat cgcccgcgag atcgaccgcg ttacgcccga
    26161 agacgggaag gtatgtgctg gcgaggcgat cctgttcgca gcgcggcggc ttcccgtatc
    26221 gggcctggag aactcctact cgcattatct gcaattgacg ccggagctcg cggcttccct
    26281 gcatgtggct ggcccggcag aagtagacaa atggatagcc ggcggcgtct tcgacacgat
    26341 tctcacgtgg cctcccgatg acccggctcc ggcccgcttc gaccttgcac ggcggtatgt
    26401 ccggcggaca aaggtgcacg actgcgagat cttctccggg aagatcgccg cgcagtgaac
    26461 aacgtcccgt gatgctcttg tcgctcgtgg tgatttgcct gacggcaggc ctgctgatct
    26521 tctcgcagac gctcgcctat tacggagacg aagcgttcca cctgctcgcg tcacaactga
    26581 tcctcctcgg caagacgcct tacgtggact tcttctaccc gcaagcgccg ctctatgcct
    26641 actggaccgc ggcgtgcatg cgggttttcg gcgatagctg gcgcacggcc cacatgttct
    26701 ccgcgctctt gaccagcgct gccatcgcgc tactccccgg atttatccgg gctgtcatac
    26761 cagaagctcg ctggcggatt ccgattgcca taacggccgt gatgctcgcc ggcctgaatc
    26821 tgctcctgat tcgatttggc acaatcggac aggcctacgg cctctgtttg tttctgattg
    26881 ccgccgcctt tcgcctcgcc gtcaaagcag cggacagttc ggcccgctgg ctcccgctct
    26941 gggcgggaat ctgcgccgga gctgcggcag cctcgtcgct gctgtgcgct ccggttgcgc
    27001 ccatcctgct ggcttggctg atccggtatg gctcccacgc caatcgctta aaaacggctg
    27061 cccggttcgc gggcggcgca gccatcccat ttcttccgct gctttggatt tctctaggag
    27121 ctccgcgtca aacgctgttc aatgttattg aatatcatct gctttaccgg aaggtgggat
    27181 atccgcatta caacatcacc ctggctgatc tcagcgtgct gagcggatgg ctcagctcgc
    27241 cgcaagggct attgctcgcc ttcctcagtg gtctaggagt gctctccaca gccgccagcg
    27301 gcgaatggag cgaagcgggc cggcgcaacc tttacctgtg catctggctg ggggccggac
    27361 tcggcgctta cctggccaca cctcacccca cttttcctca gtactttgtt cttctcattc
    27421 cgtttctcag catcctggca tcggtcgggg tctacgcaat cggctcccgg atttgccctt
    27481 tgatccggcc cgcccagctt gcgctggctg cgataggatt gtttgcactc gggctggcca
    27541 agtcggccta ccggcagcgc aacgagctgt attccctttg gccggagatt gaacagattg
    27601 ggagagtggt agaccaagtg acgccgcgga acggtttgat ttgcgccgac gaggcgattt
    27661 acgttgccgc tcaccgcgcc ccgccgccag gactggagca ctccgacgcg cacaaactgc
    27721 gtctgtcgcc tcccctggct tccctgctgc gcgtaattcc gtttgcagaa cgagatcagt
    27781 ggatatcgga agggcgtttc gataccgtcg tgatgtggtt ccccagtatg gagcgagtga
    27841 acatgctcgg tttgccgcgg ctctatgcga gatccacgaa ggttggtgat tgcctcatct
    27901 attcaggtcg aacggcggcc tcgggccgcc tcccggcaag agcgagcgcc gagaactctg
    27961 cctcactcaa tgccaaacgc tgattttttt gcgctattcc gcctgtttgt acgaaggaac
    28021 tttcttacag cggagctatg cgccgagatc cggtctgaga tgcgctcggc gtcgggaagc
    28081 gccgcaacgg tccgaaaaac gggcggcgac tacgtcgtag atcccgctgt gcgcagggcg
    28141 aagtgggtcg aggtttcggc cgcaacggtc gcccttatgg aaacgcgctt gctttctctc
    28201 aagccccaat tggaggagca ttttcatttg aagttggccg gttgccagcg gccgcagttt
    28261 ttggcgtacc gggaaggaga tttttacgct cctcatcggg acaacagcaa ggatccccag
    28321 gcggctgaaa ttcccaaaca gcgccgggtt tccgtggcta tttttctgaa cagcgagtcg
    28381 ggagaacccg ccgacggctc ctatgtcgga ggctctctca ctttttacgg cctgctgcat
    28441 gatccgcaaa agggagccgt tggattgccg cttgttgggg aaaccggtct gctgattgcc
    28501 ttccagtcca gcttagttca tgcggtggcg ccggttacct atggtgagcg gtatacaatc
    28561 gcaggttggt ttttctaggt gcgtgcgccg gcagccgcgt cggcccggct gcggcgagcg
    28621 ccgccggttt caaatcggca atgaagaata gctcgcttcg cgcaatttcc cttgtagcgg
    28681 cgctggctgc tgtgtcggcg ctcgggtgcg cgggccgggc agccgtacgg atgtttctct
    28741 ccgagcggct tatcgagacc ggatccgagc agaacctgcg gaaggcgctg cggcttgcgc
    28801 ctttgaacgc caccgcctgg tcgcagttcg gagcgctgct cgaaaatcgc ggcgatacgg
    28861 aaagcgcggc gaaaactctt cagcacgccg tggatttgga ccggtatgac tcgggagcgt
    28921 tgatcaattt aggtctccat tgggagattg gcggcgaacc gcagcgcgca gaagaatgcc
    28981 tccgggaagc ggtccgggta aatggggcct tctatccccg ctgggtgttg gccaattttt
    29041 atcttcggca gggagccgcg gaggcgttct ggaaagccat gcaggacgcc atgtccgtcc
    29101 gccgcgccga tctcacctct gtcttccaac tctactggcg ggcgtacgac gatgcggatg
    29161 aaattctgca aaaaggggtc ctggaccggc ccgacatcaa ccggcaatat tgcgacttcc
    29221 tcctcagtac agggcgtgtt ccggcggcgg ccggcgtatg gaaacgcatt gctccgcaac
    29281 tgcaagcccg cgatttgcag ttaggcttgc gctacgtaga ggctctgctg gctcaacgcc
    29341 agattgagga cggcgtcgcc gtctggaacc gcctgtgtga ggcgaagctc ctcccctacc
    29401 agggcttgga tccggcccgc gggcggctct tgaccaacgg caatttcgcc gcctctcctt
    29461 ccgcccgggg cttcgattgg aaggtgggcg agaacgaagg gattaacacg gcgctgcttg
    29521 cttccagcgc ggggccggaa ttgcgaatcc gtttcagcgg cgctcacgcc gaggccgtgg
    29581 acctgctttc gcaactggcg ccggcggcgg ccggccaagt ctaccagctc acgtttcgct
    29641 actccacggc cgctctgccg cccgataccg gcctttactg gtccatccag gacgcgctca
    29701 gcggggaaag gctcggcgag gcgccgctgg ccgcggccga ggaagcgtgg aagcaa
//
LOCUS       DASREK010000209        15212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1580382, whole genome shotgun sequence.
ACCESSION   DASREK010000209 DASREK010000000
VERSION     DASREK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 15212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15212
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580382"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFA54_10900"
     CDS             complement(<1..411)
                     /locus_tag="VFA54_10900"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZS51361.1"
                     /translation="MPVETQQAKAEHFRRLHQAPPILVLPNAWDAGSARIFEQAGFPA
                     LGTTSAGVAFALGRPDGQRISRSEMLEAVRRITSAVHIPVTADIEAGYGKTIEEILDT
                     VKGVIAAGAAGINIEDAPPDTEGVLLDLPYQTDLI"
     gene            521..2038
                     /locus_tag="VFA54_10905"
     CDS             521..2038
                     /locus_tag="VFA54_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS51362.1"
                     /translation="MKPTSCCAVFLLSFSLCARAADLRGSVQAYVGAHQAQILSELVE
                     LLSIPNIAKDRDHIRRNAGYLKQMLDRRGFDSEILETQGNPLVYGERKIPGAAHTILF
                     YAHYDGQPVDPSRWKQPSPFQPVLRDGKLEEGAKEIPNFARLQKFEDNWRVYGRSASD
                     DKSPIVALCAAIDALKAAGREPASNIRVILDGEEEAGSPSLNPAVNRYRQKLAADVAL
                     ILDGPEHASGRPTLAYGARGIVTLELTVYGPKVALHSGHYGNWAPNPAIRLAQLLASM
                     KDDQGRVLIEGFYDGIPPLTAEERRILKAVPDDEKGLQKLFGIAKPDAVGETLQEAIQ
                     FPSLNVRGLRSGYVGAEARTIIPADATADIDIRLVKETSSRQIMQKVMAHIRKQGYYI
                     VDKDPDDATRLQYPKIARVTWRRSDEGAFRTDLTLPESRRVSRALAEVWGEPPVEIRT
                     MGGTVPLSHLVEALGFPAISAPVVNFDNNQHSDNENIRLGHFWNAIVTIAALLTM"
     gene            2220..3125
                     /locus_tag="VFA54_10910"
     CDS             2220..3125
                     /locus_tag="VFA54_10910"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HZS51363.1"
                     /translation="MRHRPAARNRFLRALSLAALATAPAMAAGGAYRASIEQWRQQRE
                     AKLKADDGWLTLAGLFWLKEGENRFGTDPSNDIILPAGTTAPHAGVFDFHHGQTAVRI
                     QEPGAAVLNGRPVSTAQLKPDQPGPPDTLTIGSLTLYVIQRGARYGIRVKDKNSKTRK
                     EFAGLRYFPINESYRIVARFIPYEPPKVLAIPNILGDVEQDRSPGFAAFNLEGRQYRL
                     DAVLDDDGKSLFFIFRDLTSGKETYPAGRFLDAEPPKDGKVVLDFNKAYNPPCAFTPY
                     ATCPLPPPQNRLPVRIAAGELNYHR"
     gene            complement(3134..3430)
                     /locus_tag="VFA54_10915"
     CDS             complement(3134..3430)
                     /locus_tag="VFA54_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51364.1"
                     /translation="MRYEVVFHTTYTEDGRRLGDPTELLRLPAGVIRERSAVRVDPPL
                     ELEYPEQTEGEDLLDFAGTETWEYEVADGREQEFIEAAKNCPEVIEFSEIPQAA"
     gene            3522..3842
                     /locus_tag="VFA54_10920"
     CDS             3522..3842
                     /locus_tag="VFA54_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZS51365.1"
                     /translation="MRLTYLVCLLLALSLAAAPPQAKTTPPDKSAATGKTAAAKPPAG
                     KLIDINSASEAELKALPGIGDAYAAKIVQNRPYRAKNQLVQKKIIPQAAYDKIRDMII
                     AKQK"
     gene            complement(3891..7031)
                     /locus_tag="VFA54_10925"
     CDS             complement(3891..7031)
                     /locus_tag="VFA54_10925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HZS51366.1"
                     /translation="MYIELHARSAFSFLEGASLPEELAHCCAELGIPAMALLDRDGVY
                     GAPRFHMAAKRAGIRAHIGAEITADDGGRYSLLAATRQGYQNLCRLITCVKLRARKNE
                     AAATVEDLAGHAAGLICLWDGSQRLQALLEIFGKENVYAEIRRRFDREEEARNQAVIE
                     TARRLHIPLLAAGGVCYARPEQRELQDALTSIRHKTTIDKAGRLLAKNSERHLKPPAA
                     MERLFRDLPEAIANTEELSARLDFTLADLGYEFPKYPVPPGETMDSFLRRQTEEGARR
                     RYRPYHERARKQIERELALIEKLGLAGYFLIVWDIVQFCRRQGILVQGRGSAANSAVC
                     YSLGITAVDPVGMDLLFERFLSEERGEWPDIDLDLPSGDQRERAIQYVYERYGQRGAA
                     MTANVITYRGRSAAREIGKALGFDPAMLARLSSLAGAWEWKDPYDPAEKQFREAGLDL
                     SHPRIQKFFELYRAVQDLPRHLGQHSGGMVICQGQLDSIVPLEPASMPGRVVVQWDKD
                     DCADMKIIKVDLLGLGMMAVIEDSLALIRDAYGEEVDLAHLPQDDPAVYDALQRADTI
                     GMFQVESRAQMSCLPRLCPKKFYDIVVQVAIIRPGPIVGKMVNPYLKRRQGREPVEYM
                     HPSLEPVLKRTLGVPLFQEQLLRMAMIAASFSGGEAEELRRAFGFKRSERRMQEIEVK
                     LRRGMERNGITGRTQDAIVESIASFALYGFPESHAASFALLAYASAYLKCHYLAAFTA
                     ALLNNQPMGFYHPSTIIKDAQRHGLKIKPIDVAHSGWLCTIEEEPGMEMRRPENTAGA
                     EEIAAHDSAPALKKCLRLGLRYVKGLRQQTAEAIVRERAQRPFANIDDLARRVPELRQ
                     DEIRRLAEIGALNSIGAGDRRDALWQAQRAGRPAGPLLDRLPESDSTSPLRPMTPEER
                     LLADFRSTGLTIGPHPMAYRRAEMNQLRVSRAVDLRNIPDGRRVRVAGCVIARQRPGT
                     AKGFLFLSLEDETGIANAIVTPDLFEDRRFVLVNEPFLLIDGVLQNQDGVVSVKAQRV
                     QPLAAPEAAGESHDFR"
     gene            complement(7127..8656)
                     /locus_tag="VFA54_10930"
     CDS             complement(7127..8656)
                     /locus_tag="VFA54_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51367.1"
                     /translation="MFACLHAPELPDSFHALLIHCACGFSPRVEDTGAQTAVLDIEGL
                     DRLLGSPRQIAEAMARRAGELGIPANVAVASNPDAAVHAARGFRGITVIPKGLEASVL
                     ASLPVEVLSPPPEILETLESWGIRRFGELAALPETGVAARLGAEGVRLHKLARGVGER
                     ALAPAIPAETLEAGIELEHPVALLEPLSFLLARLLDELLEKVEARGLAVDELRLRLKL
                     EPRGEDLEAGRAPAESALPETAGGDPLQEKSRRAGYGESSGILRQSKISIEHARTIRL
                     PFPMRSAKTFLKLLASDLETHPPQAPVIAVSLAAVPADPRLVQYGFFLPPAPEPEKLE
                     LTLGRIRKLVGAENVGAAELLDTHRPQAFRVRPFAVPQRSHTLGWRASGYFPKKDAGE
                     ASPGKAQSASRAEPGSFAEASFSEAGRSPHRSLALRVFRPPLRAHVEAPSGRPARIVA
                     RGLHAPVISQAGPWRSSGDWWTAGGWSRDEWDVALSDGAIYRIYLDRLSGAWFVEGYY
                     D"
     gene            complement(8666..9430)
                     /locus_tag="VFA54_10935"
     CDS             complement(8666..9430)
                     /locus_tag="VFA54_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51368.1"
                     /translation="MQSPLRKTDLESVLGGHFAWPGRMGVHPGLSPPAPERVSTGIAE
                     VDALTGGLPRGAITEIFGPASSGRTSLLAATLARATQRGEVCALVDTNNTFDPLSAAQ
                     AGIDLGRLLWVRCTGSPDGPRRRWKTHPAEQALQVTDWLLQGGGFSIAALDLGDVPPR
                     IARRISLTSWFRLRRAIENTPSILIVIEQEPLAKTCASLVLEIQKEKTNWAGPLCPGR
                     LLEGTLHRLAARKPVRSAAAAFTLRAAWTSKQIAVG"
     gene            9632..10309
                     /locus_tag="VFA54_10940"
     CDS             9632..10309
                     /locus_tag="VFA54_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51369.1"
                     /translation="MKIVVAVFTSRDAAERAAQKLLPLGIPHEHISLLSPDHIEQQVE
                     AVPVIQAEQPGIGGALGGLVGGAAGIAGGVQLGMAAASVMVPGVGPVLAIGLFGAVLF
                     GATGALTGAALGKSLDDFLSEGLPEDELYVYKDALRKGHSVVIALTEGESQTGDIRRA
                     LAEAGAESVDQARQAAWLGLHNAASEKYADEQHSSNGHASPESAEAKAEQGKETKAGR
                     RSRSRKS"
     gene            complement(10490..12475)
                     /locus_tag="VFA54_10945"
     CDS             complement(10490..12475)
                     /locus_tag="VFA54_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51370.1"
                     /translation="MFPDEGGPAAQAAMFSETDPGGLRRGRFLYFPAVPGRVEFAEEV
                     RRVILAERPDVVAVELPVTLEGAYRRALERLPELSVIVYEDGDSEQDAPRAVYIPVEV
                     TDPFVEALRTAAETGAQTAFIDPDTAERPHLDEEYPDTYAVARIGLEKYVAAYRFHKR
                     ERSKRVSAFAEGIAWKLQGTDPLARVLVVVSLNLLDAVMEAMEHPQAQPLRRIKRQGV
                     RTLNLHPDCLAEVLTEYPFLQAVYERRRSGALTPDVSGAPIAHRSDGLEVWQAPKLDP
                     RQAAVERAANHDFGGRLCERQHMQFRLLAEAERQYEANTGEKLAHWQRRLWSRYSRNL
                     AFTQRQLMADLFDLTVAARSIADDNFAWELWEMANAYPYQKTATDLITVQISGEEMWV
                     KTKRILLRRRLPRKKARLRPVGLRGRKKETFPGEWEKSRRGRWSICSYPPEDLVIENY
                     GDFLKKKGKSILSEERARTEPFTTSLLDGVDLRETIRNWHEGKLYVRSFQKIAGEVGA
                     VVVIFDEDRDDRYHYCITWLGEHQNESDMAFYATDPMSHMVGPGIGRAEYGGFLLSLP
                     PLRMTDIWSDEDYAFARSKPERLLLAALDYSLEKNVVYVAPRPPRSVFRTIAGRLGRK
                     VIYIPIGQLSPVALKKIRVFHVLDGHDRREIAKDYIW"
     gene            complement(12462..13310)
                     /locus_tag="VFA54_10950"
     CDS             complement(12462..13310)
                     /locus_tag="VFA54_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZS51371.1"
                     /translation="MKIPVEESPRTVVIEGVTLHLAHPDEIPIRWVGQQELIRQLLAA
                     WMTVDKRDVPMSPRLLGKPGVGKTTLAYAAATQLGREVYIMQATLDTRPEDLLVTPVI
                     EAGGKLRYVASPLVTAMLRGGVVVLDEGNRMSEKSWASLAPLLDTRRYVESVVAGIKI
                     HAHPDFRLVATMNDDASTFELPEYIHSRLQPQIMLDFPEREEELLILKENLPFAGEQV
                     LDYVAGFLQSAHRADERYTVRDGINIARFAIKLAQTQAGSGIEEALETALIQTLGREA
                     LRYVPR"
     gene            13594..15087
                     /locus_tag="VFA54_10955"
     CDS             13594..15087
                     /locus_tag="VFA54_10955"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZS51372.1"
                     /translation="MKWRHGFGAVAFLLAASVLCAQAQPERGRAGRSAPTRRSMPCPA
                     AAGGDPDGDYIIARTVADVPYGRGQKMDVYAPAGEPRPAAVVIRGRHGNQFDFVTKLY
                     EQLTRAGYAWFVPDYRDDGDLAAALDFIRCPGRFAITNTLILVGEDNGAAAALRSAAR
                     DSRVTGVVTVAASLGGDAGASEAMPQVPVLMIQGDQDKEFTPRQAQNLCSRLKSCNVY
                     VQSPAPHAFEQWHPQTEDYQEELDAWLRGDQRGLWKDISYSRPGGRDLPMDAFIPRGA
                     GPFPAVIIVHGGGFARDKVTYIAPIFEPLARANIAWFSIDYTPLPYIHRPEQLEQLRE
                     AIRYVKRHARRYNIDPNRLALMGESFSAPMVTIVASKPCPGCEVQAVLSFYGSYKAAI
                     PQDAAARARLDAMYGPGAWTPETLHEYAPYDLAHPGMPPVLVVQGTGERGFERSREYY
                     EHLKELGVPTELVLVPGAPHGIENWEGRPEWAFYKKKVVDWLKATWR"
BASE COUNT         2931 a         4857 c         4667 g         2757 t
ORIGIN      
        1 gatcaaatcc gtttgatacg gcagatccaa aagcacgccc tccgtatccg gcggagcatc
       61 ctcgatattg atgccggccg cccccgcggc gatgacgccc ttcacggtgt caagaatttc
      121 ttcgatcgtt ttcccgtagc cggcctcgat gtcggcggtc accgggatat gtactgctga
      181 tgtaatgcgc cgcacggctt cgagcatttc cgagcggctg atgcgctggc cgtcgggacg
      241 cccgagtgcg aacgccacgc cggcgctcgt ggtgccgagc gcgggaaagc cggcttgttc
      301 aaaaatccgg gcgctgcccg cgtcccaggc gttgggcaag accaaaatcg gcggcgcctg
      361 gtgaagtctc cggaaatgtt ccgcttttgc ctgctgcgtc tcgaccggca tctcatccct
      421 ccgtgatctt ggaaaccaac tgccctcccc gccacctcag tctttagccc gatcagcagc
      481 cgggcgcttc cggccgggtg atggtgtata ctcgcggctg atgaaaccga cgtcgtgctg
      541 cgccgttttt ctgctttcct tttccctttg cgcgcgtgcc gcagacctgc ggggaagcgt
      601 tcaagcgtac gtgggcgcgc accaggcgca gatcctctcc gaactggtcg agctgctctc
      661 gattcccaat atcgccaagg atcgtgacca catccgccgc aacgccggat acctgaaaca
      721 gatgctggac cgccggggct tcgattcaga aattctcgag acccagggca atccgctggt
      781 ttacggcgag cggaaaattc cgggagcggc ccataccatt ctcttctatg cccactatga
      841 cggccagccc gtcgatcctt cgcgctggaa acagccgagc ccgtttcagc ccgtgctgcg
      901 cgacggcaag ctggaagagg gcgctaagga gattccgaat ttcgcgcggc tgcaaaaatt
      961 cgaagacaac tggagggttt acgggcgctc cgcgtcggac gataaatccc cgattgtcgc
     1021 gctctgcgcg gctatcgatg cgctgaaagc cgccggccgg gagcctgcca gcaacatccg
     1081 ggtgatcctg gatggcgaag aagaagcggg atcgccgagc ctgaacccgg ccgtcaaccg
     1141 ctatcgccag aagctggccg ctgatgtggc gttgatcctg gatggtcccg agcatgcgag
     1201 cgggcggccc acattggcct acggcgcgcg cggcatcgtc actctcgaat tgacggttta
     1261 cgggccgaaa gtggctctcc acagcggcca ttatggcaac tgggcgccca atcctgccat
     1321 tcgtctcgcg caactgctgg cctccatgaa ggacgaccag ggacgcgtgc tcatcgaggg
     1381 cttctatgac ggcatcccgc cattgaccgc cgaagagcgc cgcatactga aagctgtccc
     1441 cgacgacgag aaagggttgc agaagctatt cggcatcgcc aagccggacg cggtgggcga
     1501 gacgttgcag gaagcgattc agtttcccag cctgaacgtg cgcgggctgc gcagcggcta
     1561 cgtgggcgcc gaggcccgca cgatcattcc tgccgacgca accgccgaca tcgacatccg
     1621 gctggtaaaa gaaacctcga gccggcagat catgcaaaag gtgatggcgc atatccgcaa
     1681 acagggctac tacatcgttg ataaagatcc cgacgatgcc acccgcctgc aatatcccaa
     1741 gatcgcgcgc gtgacgtggc gccgcagcga cgaaggagcc tttcgcaccg acctcacgct
     1801 gccggaatcg cggcgagtga gcagggccct ggcggaagtg tggggcgagc cgccggtgga
     1861 aatccgaaca atgggcggca ccgttccact gagccatctg gtcgaagccc tcggcttccc
     1921 ggcgatcagc gcgcccgtgg tgaactttga caacaaccag cacagcgaca atgagaatat
     1981 ccgccttggg cacttctgga atgcaatcgt gacgatcgcc gctctgctca cgatgtagaa
     2041 acgctgaagc ggcgatccgc ccgcccctct gcggccgctg ctacactaga tttaccagcg
     2101 caggcgcttg caggcgcgca ttcccgcgct gcagacggcg caggcttgcg ccgggaagat
     2161 cgcaccgcag gtcgtgcggg agccggttgc gcctcaagtt gcgggagcgc caattattga
     2221 tgagacatcg gccggcagcg cggaatcgat ttctccgggc gctttcactc gcggcgcttg
     2281 cgaccgcgcc ggcaatggcc gccggcggcg cctaccgggc ttcgatcgag cagtggcggc
     2341 agcagcgcga agcaaaactg aaggcggacg acggctggct gactctggcc gggcttttct
     2401 ggctgaaaga aggagaaaac cgcttcggaa cagacccttc gaacgacatc attctcccgg
     2461 cagggacgac ggcgccgcac gccggcgtgt tcgattttca ccacggccag acggcggttc
     2521 gcatccaaga gcccggagca gcagttctca atggcaggcc ggtctcaacc gcccaattga
     2581 agccggacca gccgggtcct cccgacactc ttaccattgg cagcttgact ctttacgtga
     2641 tccagcgcgg tgcgcgctat ggcatccgcg tgaaagacaa gaacagcaag acgcggaagg
     2701 aatttgcagg cctgcgctat tttccgataa acgaatctta tcgcatagtg gcgcgcttca
     2761 tcccttacga gccgccgaag gtgctcgcca ttcctaacat cctcggcgat gtcgagcagg
     2821 atcgcagccc gggcttcgcg gctttcaatc tggagggccg ccaataccgg ctcgacgccg
     2881 tgctggacga tgacggcaag agcttgtttt tcattttccg agacctcacc agcgggaaag
     2941 agacataccc ggccggacgc tttctggacg ccgagccgcc gaaagacggc aaagtcgttc
     3001 tcgatttcaa caaagcttat aatccgccgt gcgccttcac cccgtacgcc acctgcccat
     3061 tgccgccccc gcagaatcgt ctacccgtcc gcatcgcggc cggagaattg aattaccatc
     3121 gctgacaaaa aaatcaggca gcttgcggga tctcgctgaa ttcgattacc tcggggcagt
     3181 tcttcgccgc ctcgatgaac tcctgctccc gcccgtcggc cacttcgtac tcccaggttt
     3241 cggtgccggc gaagtcgagc agatcctctc cctcggtctg ctccggatac tccagttcca
     3301 gcggaggatc cacccgcacg gccgatcgct cgcgaatgac gccggcggga agacgcaata
     3361 actcagtcgg gtcgccgagc cgcctgccgt cttcggtgta cgttgtatgg aagaccactt
     3421 cgtagcgcat cagccgacct cctttgcttt cagcttaaca atgcgggcga ccgatgcggc
     3481 cgaggatcgt acaatgaggg ctggatgatg ggagaagagc catgagactg acgtatttgg
     3541 tatgcctgct gctggcgctg tcgctggcag cggccccgcc gcaggcgaag accaccccgc
     3601 ctgataaatc ggctgccacg ggcaagaccg ctgccgcaaa gccgcccgcg ggcaaattga
     3661 tcgacatcaa ctccgccagc gaggcggagc tgaaggcgct tccaggcatt ggcgatgcct
     3721 acgcagccaa aatcgtccaa aaccgtcctt acagggccaa gaaccagctc gtgcagaaaa
     3781 aaatcatccc gcaggccgcc tacgacaaga tccgagacat gattatcgcg aaacagaaat
     3841 agccgtgacg ccgatcgaat gatcgtgttt ggcggaacga aaaacgaagg tcagcgaaaa
     3901 tcgtgcgatt cgccggcggc ttcgggcgcc gccagcggct gtacgcgctg agcctttact
     3961 gaaaccacgc cgtcttgatt ttgcagcacg ccatcgatca gaagaaacgg ctcgttcacc
     4021 agaacaaacc ggcggtcctc gaaaagatcg ggagtgacga tggcgttggc aatgccggtt
     4081 tcatcttcca ggctcaaaaa taaaaatcct ttggccgtgc ccggcctctg gcgggcgatc
     4141 acgcagccgg ccacgcgcac gcggcggcca tcgggaatat tccgcaaatc caccgctcgg
     4201 gaaacgcgaa gctgattcat ctccgcccgc cggtaagcca tgggatgcgg cccgatggtc
     4261 agcccagtcg agcgaaaatc cgccaacagg cgctcctcag gcgtcatcgg ccgcagtgga
     4321 gaggtggagt cgctctccgg caggcggtcg agcagcggcc cagccggccg cccggcgcgc
     4381 tgggcctgcc aaagggcgtc ccggcggtca cccgcgccga ttgagtttag cgcgccgatt
     4441 tcggccagcc ggcgaatctc atcctgtcgc agttccggca cgcggcgcgc cagatcgtca
     4501 atgttcgcaa acggcctctg cgcccgctcg cgcacaatcg cctcagccgt ctgctgccgc
     4561 agccctttta catatcgcag ccccaggcga aggcatttct tcaacgccgg cgccgagtcg
     4621 tgcgcggcta tctcttccgc gcccgccgta ttttccggac gccgcatctc catgcccggc
     4681 tcttcttcaa tcgtacacag ccagccggag tgcgccacgt cgatgggttt gatcttgagc
     4741 ccgtgtcgct gggcgtcttt gataatcgtc gaaggatggt agaaacccat cggctggttg
     4801 ttgagcaaag cggcggtgaa cgccgccagg taatggcatt tgagataggc gctcgcatag
     4861 gccagcagcg cgaagctcgc ggcgtgggac tcaggaaaac cgtagagcgc aaaggaagcg
     4921 atggattcca cgatcgcgtc ctgcgtgcgg ccggtgatgc cgttgcgctc catgccgcgc
     4981 cgcagtttca cctcgatttc ctgcatgcgc cgctccgagc gcttgaagcc gaaggctcgc
     5041 cgcagctctt cggcttctcc gccggaaaac gacgccgcga tcatcgccat acgcagcaac
     5101 tgttcctgaa acagcggcac tcccagcgtt cgcttgagca ccggctcgag cgacgggtgc
     5161 atgtattcga ccggctcgcg cccctggcgg cgcttcaaat acgggttcac cattttgccg
     5221 acaatcggcc ccgggcggat gatcgccacc tgcaccacga tgtcgtaaaa ttttttcggg
     5281 cacaagcgcg gcaggcagga catctgggcg cggctttcca cctggaacat gccgatcgta
     5341 tccgcccgct ggagcgcgtc gtaaacagcg gggtcgtcct ggggcaggtg cgccagatcc
     5401 acctcttccc catacgcatc gcgaatcaaa gccagagagt cttcaatcac cgccatcatt
     5461 cccaggccca gcaggtccac cttgatgatc ttcatgtcgg cgcaatcgtc tttatcccat
     5521 tgcacgacga cgcgtccggg catggaggcc ggctcgagcg gcacgatgga atcaagctgg
     5581 ccctggcaaa tcaccatgcc gccggaatgc tggcctaaat gccgcggcag gtcctgcacg
     5641 gcgcgataga gctcgaaaaa tttttgaatg cgcggatggc tcagatcgag acccgcctct
     5701 cgaaattgct tttccgccgg gtcataggga tctttccatt cccaggcacc ggccaggctc
     5761 gaaagccggg ccagcatagc cgggtcgaag cccagcgcct tgccgatctc gcgcgccgcc
     5821 gagcgccctc tgtaggtaat gacattggcg gtcatggccg cgccgcgctg gccgtagcgc
     5881 tcgtaaacat actggatggc gcgctcgcgc tgatcgccgc tcggcaaatc gagatcgata
     5941 tccggccatt cgccccgttc ttcagaaaga aaacgctcaa agagcaggtc catgcccacc
     6001 ggatcgaccg ccgtgatgcc gagcgagtag caaaccgcgc tgttggccgc cgagccgcgg
     6061 ccctgtacga gaattccttg ccggcggcag aattgtacga tgtcccagac gatcaaaaaa
     6121 tatccggcaa gcccgagctt ttcaatcagc gccagctcac gctcgatctg cttccgcgcg
     6181 cgctcgtgat aagggcggta gcggcggcgt gcgccctcct cggtctgcct gcggagaaac
     6241 gagtccatgg tttcgcccgg cggcacggga tacttcggaa attcgtaacc caaatcggcc
     6301 agcgtaaaat cgagccgcgc ggaaagctct tcggtattgg caatagcttc cggcaaatcg
     6361 cgaaacaggc gctccatggc ggcgggaggc ttgagatggc gctcggaatt tttcgccagc
     6421 aggcggccgg ctttatcgat cgtcgtcttg tgccggatcg acgtgagcgc atcctgcaac
     6481 tccctctgct cgggtcgggc gtagcagacg ccgccggcgg ccagcagcgg gatatggagc
     6541 cgccgggcgg tctcgatcac cgcttgattg cgcgcctcct cctcgcggtc gaagcgccgc
     6601 cgaatttcgg catagacgtt ttccttgccg aaaatttcca gaagagcttg cagccgctgg
     6661 cttccgtccc acaagcagat caatcccgcg gcgtgccccg ccagatcttc gaccgtcgcg
     6721 gcggcttcgt ttttcctggc gcgcagcttc acgcacgtga tcaggcggca caggttttgg
     6781 tagccctgcc gggtggcggc aagcaacgag taccggcctc cgtcgtccgc ggtgatctct
     6841 gcgccgatat gcgctcgaat gccggctctt ttcgccgcca tatgaaagcg cggcgctcca
     6901 tatacgccat cccggtcgag cagcgccatg gcgggaattc ccagttccgc gcagcagtgc
     6961 gccagctctt cgggcagcga agcgccctcg agaaaactaa aagcggagcg ggcatgaagc
     7021 tcgatgtaca tagcgggaaa attcttcggc agcagtctgt gccctatttg cgctttccat
     7081 ccggtacaaa aacaagccgg ccttccggtg cggcggccgt cctgcgtcag tcgtaatacc
     7141 cttccacaaa ccaggcgccg ctgagccggt ccagataaat gcggtaaatc gctccatcgc
     7201 tgagcgccac gtcccattca tcgcgcgacc agccgccggc ggtccaccaa tcgcctgaac
     7261 tgcgccacgg ccctgcctgg gaaatcacag gggcatgcag gccccgcgcc acgatgcgcg
     7321 ccggccggcc cgagggcgct tccacatggg ctcgcagcgg cgggcggaaa acacgcaacg
     7381 caagagaccg atgtgggcta cggcctgctt cggaaaacga ggcttccgcg aagcttccgg
     7441 gttcggctct agaggcgctc tgcgctttcc cgggagaagc ttcgccggcg tcttttttcg
     7501 gaaaatatcc agaggcgcgc cagcccagcg tgtgggatcg ctgcggaaca gcaaagggcc
     7561 ggacccggaa cgcttgcggc cggtgtgtgt ccagcagctc cgccgcgccg acattctcag
     7621 cgcccaccag cttcctgatc cggcccaaag tcaactcgag cttttccggt tccggcgcgg
     7681 gcggcaggaa gaatccgtat tgcacaagac gcggatccgc cggcacggcc gccagcgaaa
     7741 ccgcgatcac cggcgcctgc ggcggatgcg tttccagatc ggacgccagc agcttcaaaa
     7801 aagttttggc gctgcgcatc ggaaagggaa ggcggatcgt gcgcgcatgc tcaatcgaaa
     7861 ttttcgattg ccgcaaaatt ccggagcttt ctccgtaacc ggcgcggcgg cttttctcct
     7921 gcaacggatc gccgccggct gtttccggca gcgcgctttc agccggcgcc cgcccggctt
     7981 ccaaatcttc gccgcgcggc tcgagcttga gccgcagccg caattcatcc accgccaagc
     8041 cccgcgcttc gaccttctcc agcaattcgt ccagcagccg ggccaagaga aaagaaagcg
     8101 gctctagcag agccaccgga tgttctagtt ctatgccggc ttccaacgtc tccgccggaa
     8161 tcgccggcgc cagcgcccgc tctccaactc ctcgcgccag tttgtgaagc cgcacgcctt
     8221 ccgcccccaa ccgcgccgca acgcccgtct ccggcagtgc cgccaattcg ccaaaacgcc
     8281 ggattcccca gctctcgagc gtctccaaaa tctccggagg cggtgaaagg acctccaccg
     8341 gcaggctcgc cagaacgctg gcctccaggc ctttcggaat aaccgtaatt ccgcggaagc
     8401 cgcgtgcggc atgcaccgca gcatccggat tcgaagccac ggcaacattc gccggaatcc
     8461 ccagttcccc ggcccgccgc gccatggctt ccgcaatctg cctgggggat cccagcagac
     8521 gatcgagacc ctctatgtcg agcacggccg tttgggctcc ggtgtcttcc acccgagggg
     8581 aaaatccgca ggcgcaatga atcagcagcg cgtgaaacga atccggcagt tccggcgcat
     8641 gcaggcaggc gaacatatcc tttccttatc ccaccgcgat ttgcttgctt gtccatgccg
     8701 cccgcagcgt gaaggccgcg gccgctgaac gaaccggctt gcgcgccgca agccggtgca
     8761 gcgtgccctc gagcaggcgg cccgggcaga gcggccccgc ccaattcgtc ttttcttttt
     8821 gaatttccag caccagcgaa gcgcaggttt tcgccagcgg ctcctgctcg atcacgatca
     8881 gaatggacgg cgtgttctcg atcgcccggc ggaggcgaaa ccaggacgtc aatgagatgc
     8941 gccgcgctat gcggggcgga acatcgccca gatcgagcgc ggcaatgctg aagccgcctc
     9001 cctgcaacag ccagtccgta acctgaagcg cctgctcggc gggatgcgtt ttccagcgcc
     9061 ggcgagggcc gtccggggac cccgtgcagc gcacccaaag cagccggccg agatcgatcc
     9121 cggcctgggc ggcggaaaga ggatcaaaag tattgttagt atctacaaga gcgcaaacct
     9181 cgccccgttg ggtggcccga gccagcgttg cagccagcag actggtgcgc ccggaagagg
     9241 ccgggccaaa aatctcggtg atggctcccc gcggcagacc tccggtcaag gcatctacct
     9301 ccgctatgcc tgtggaaacc ctttctggag caggcgggga aagccccggg tgaaccccca
     9361 tccggccggg ccacgcgaaa tggccgccca acacggattc gaggtccgtt ttcctgagcg
     9421 gggattgcat tggggatcct gctgtgctcc agcgttttat tcgcttttta ttcgccttac
     9481 cgagccattc tccctttccg ctcggggagc tgtcaagagc gcttcttaca aatcagataa
     9541 gaatttattt ttcaattaga tcgaaacgta ataagacgca cggcacagcc cggctgtaag
     9601 gttcaaaata aaactgaagg agggcgcaga gatgaaaata gttgtagccg tcttcacctc
     9661 ccgcgatgcg gccgaacgcg ctgctcaaaa gctgcttccg ctcggcattc cgcacgagca
     9721 tatcagcctg ttgtcgcccg atcacatcga gcagcaagtc gaggctgttc cggtgatcca
     9781 ggcggagcaa cccggcatag gcggagcctt aggaggcctg gtcggcgggg cggcaggcat
     9841 cgccggcggc gttcagttag gcatggccgc ggcgagcgtt atggtgcctg gagtcggtcc
     9901 cgtactggcg atcggccttt tcggcgctgt tctgtttggc gcaactggag ctttaacggg
     9961 agctgccctc ggcaaaagcc tggacgactt cctcagcgag ggattgccag aggacgagct
    10021 gtacgtctac aaagacgctc tccgcaaagg ccactctgtg gtcatcgctc tgactgaggg
    10081 cgaatcgcag actggcgaca tccgcagagc tctggccgag gccggagccg aaagtgtgga
    10141 ccaagcgcgc caggcagcgt ggctcggcct tcacaatgca gcgtcagaaa aatacgcgga
    10201 cgaacagcat tccagcaacg ggcatgcctc tccggagtcg gccgaagcaa aagccgagca
    10261 gggcaaagaa accaaggccg gccgaaggtc caggagcagg aagagctgat tggcggcaga
    10321 cggcggagct ctgtgccgcg tgcgccaagg cggggtccga attggctgag tcggaattgc
    10381 attcctctgg gagccgggac agaaaacgtc cttccgcctc gctgcaatgg cccggctttt
    10441 atccggcagt cagagattcc agcgctcgtc aggccggaaa gccagatagc taccagatgt
    10501 agtctttcgc tatctcccgc cggtcgtggc cgtcgagcac gtggaagaca cgaattttct
    10561 tgagagctac gggcgagagc tggccgatgg gaatataaat gactttccgg cccagccggc
    10621 cggcgatggt gcgaaagacg ctgcggggag ggcgcggggc gacgtagacg acattttttt
    10681 cgagagaata atcgagcgcc gcgagcagga ggcgctccgg cttcgatcgg gcaaacgcgt
    10741 aatcttcatc gctccagata tcggtcatcc gcaggggcgg aagagatagc agaaagccgc
    10801 cgtactcagc ccgcccgatg ccgggtccga ccatgtggct catggggtcg gtcgcgtaga
    10861 acgccatgtc gctttcgttc tggtgctcgc cgagccaggt aatgcagtag tgatacctgt
    10921 cgtcgcggtc ttcatcgaag attactacta cggcgccgac ttcgccggcg attttctgaa
    10981 atgagcgcac gtagagcttg ccttcgtgcc agttgcggat cgtttcccgc agatcgacgc
    11041 cgtcgagaag ggatgtggta aaaggctcgg ttcgcgcgcg ctcctcggag aggatgcttt
    11101 tgcctttttt cttgagaaag tcgccgtagt tctcgatcac cagatcctcg ggagggtacg
    11161 agcagatcga ccaccggcct cgacgggatt tttcccactc gccggggaaa gtttcctttt
    11221 tccgcccgcg caagcccacc ggccggagcc gggctttttt gcgcggcaag cgccgccgca
    11281 gcagaatgcg cttggttttg acccacattt cttcaccgga gatctgcaca gtgatcaggt
    11341 cggtggcggt cttctggtaa ggataggcat tggccatctc ccacagctcc caggcgaagt
    11401 tgtcgtcggc aattgaccgt gcggcgacgg tcagatcaaa caaatcggcc atcaattggc
    11461 gctgggtgaa cgcgaggttg cgcgagtagc gggaccagag ccggcgctgc cagtgagcga
    11521 gtttctcgcc ggtgttggct tcgtattgcc gctcggcttc cgccagcagg cggaattgca
    11581 tgtgctggcg ttcgcagagc ctgccgccga agtcatgatt cgctgcgcgt tccacggccg
    11641 cctgccttgg gtccagcttc ggggcttgcc atacttccag gccgtcgctg cgatgcgcga
    11701 tcggcgctcc cgacacatcc ggggtcaaag ctccggagcg gcggcgttca tacaccgcct
    11761 gcaagaaagg atattccgtg aggacttccg ccagacaatc ggggtgcaga ttgagcgtgc
    11821 gaacgccctg ccgcttgatg cgccggaggg gctgcgcttg cgggtgctcc atcgcttcca
    11881 tcaccgcatc gagcagattg aggctgacga ccaccagcac gcgcgccagc ggatccgttc
    11941 cctgcaattt ccaggcgatg ccttcggcga aagcgctcac gcgcttcgag cgttcccgct
    12001 tgtggaagcg gtaggcggcc acgtactttt ccagcccgat cctggcgacg gcataggtat
    12061 cgggatactc ttcgtcgaga tgcgggcgtt cggcggtgtc gggatcgatg aaggcggttt
    12121 gcgccccggt ttccgcggcg gttctcagcg cttcgacaaa aggatcggtc acctcgaccg
    12181 ggatatagac ggcgcggggc gcgtcctgtt ccgaatcgcc gtcctcgtaa acgatcacgg
    12241 agagctcggg cagccgctcg agcgcgcggc ggtaagctcc ttccagagta acggggagtt
    12301 ccacggcgac cacatccggc cgctcggcca gaatgacgcg gcgaacttct tcagcgaatt
    12361 ccacacggcc gggcactgcg gggaagtaga ggaaccggcc tcggcgcagc ccgccgggat
    12421 cggtctcgga aaacatggca gcctgagcag ccgggccgcc ctcatcgggg aacataacgc
    12481 aacgcctccc ggccgagcgt ctggatcagc gccgtctcca gcgcctcttc gatgccggag
    12541 ccggcctgcg tctgcgccag cttgatggcg aagcgggcga tattgatgcc gtcgcgcacg
    12601 gtgtagcgtt cgtcggcccg gtgcgccgat tgcaaaaacc cggccacgta atcgagcacc
    12661 tgctcgccgg cgaacggcag attctctttg aggatgagca gttcttcctc gcgctcgggg
    12721 aagtccagca tgatctgcgg ttggaggcgc gagtgaatat attccggcag ctcgaaagtc
    12781 gaggcgtcgt cgttcatggt ggcgaccagg cgaaagtccg ggtgcgcgtg gatcttgatg
    12841 ccggccacga cggattcgac atagcggcgc gtgtcgagca ggggcgccag agacgcccag
    12901 cttttttcgc tcatccgatt gccttcgtcc agcacaacga cgccgccgcg gagcatggcg
    12961 gtcaccagcg gcgaagcgac atagcggagt ttcccgcccg cttcaatgac gggcgtcacc
    13021 agcaggtctt ccgggcgcgt atcgagcgtg gcctgcataa tatagacctc gcggccgagt
    13081 tgcgtggccg ccgcgtaggc cagcgtggtc ttgccgacgc cgggcttgcc gagcaggcgc
    13141 ggactcatcg gcacgtcgcg cttatctaca gtcatccagg cagcgagcaa ttgcctaatc
    13201 aactcctgct ggcccaccca tcggatcggg atctcatccg gatgcgccag atggagagtc
    13261 acgccttcga taacgacggt acgcgggctt tcctcgacgg gaatcttcat atttataggg
    13321 ccccaaattc tattctagat ctactccggg ccccgcactt acggatgccg gaagcatccc
    13381 cggccacggc gtcaagccac aacagcgtgc aaaatgcgtt ccggggcggg ttgatcgctc
    13441 atttttgatc ccctttccga tttccaaacc cttcatgata gcgtccgaca catgctgtcg
    13501 cgaaaaataa cctccgccgg atcagaaatt gcaaaacgcg gcgagcgggc cgaagtatac
    13561 tgacagaatc ggcgtgagtg acggagggcg gctatgaaat ggcggcatgg atttggagct
    13621 gtcgcgttcc tgctggccgc ttcggttctc tgcgcccaag ctcagccgga gcgaggccgc
    13681 gctgggcgga gcgcgccgac gcgccgatcg atgccgtgtc cggcggccgc gggcggcgat
    13741 cccgacggcg actacatcat cgccaggact gtggctgacg taccttacgg ccgcggccag
    13801 aagatggatg tctacgcgcc cgccggggag ccgcggcctg ccgccgtcgt gattcgaggg
    13861 cgccacggga accagttcga cttcgttacg aagctttacg agcagctcac ccgcgcgggc
    13921 tacgcatggt ttgttcccga ttaccgggac gacggcgacc ttgcggcggc cttggacttc
    13981 atccgctgcc ccggccgctt tgctattacc aatactctca ttctggtggg ggaggacaat
    14041 ggggcggcgg cggcgctgcg atccgccgca cgagacagcc gggtgacagg cgttgtgaca
    14101 gtggccgcca gcctgggcgg agacgccggg gcaagcgaag caatgcccca ggttccagtg
    14161 ctgatgatcc agggagacca ggacaaggaa tttactccac gccaggccca aaacctttgc
    14221 agccgattga agagctgcaa cgtctacgtc cagagcccgg ccccgcacgc gttcgagcag
    14281 tggcatccgc agacggaaga ctatcaggaa gagctggacg cgtggctgcg gggcgaccag
    14341 cgcggcctgt ggaaagacat ttcctattcg cgtcccggcg ggcgagacct gccgatggac
    14401 gccttcatcc cgcggggcgc cggaccattt ccggccgtaa tcatcgtgca cggcggcggc
    14461 ttcgcgcggg acaaagtcac ctatatcgcg cccatcttcg agccgctggc ccgggccaac
    14521 atcgcgtggt tttccatcga ctatacgccc ttgccctaca tccaccggcc ggagcagctc
    14581 gagcaactac gcgaagcgat ccgctacgtg aagaggcacg ccagacgcta caacatcgat
    14641 cccaaccgcc tggcgctgat gggcgaatct ttcagcgcac cgatggtgac gattgtcgcc
    14701 tccaagccat gcccgggctg cgaagtgcag gcggtgctgt cgttctacgg cagctataag
    14761 gcggcgatcc cgcaagatgc ggccgcccgc gcccgcctgg atgcaatgta cgggccgggc
    14821 gcctggactc cggaaaccct tcatgagtac gccccctacg atctcgccca tcccggcatg
    14881 ccccctgtct tagtagtaca agggacgggc gagcgaggct tcgagcgatc ccgggaatat
    14941 tacgagcact taaaggagct tggcgtcccg acggagctgg tgcttgtgcc aggcgcgccg
    15001 cacggcatcg agaactggga aggccgcccg gaatgggcgt tctataaaaa gaaagtcgtc
    15061 gactggctga aggccacatg gcggtaaggc tctcgtccgg ctcaacgggc cgcgggcgaa
    15121 tcccagtttt cccggcgact cctgcggcgt tgaaggggcg ctccggcggc aggttgttta
    15181 gacggcggca gacggagggc ggcgtgaacg gg
//
LOCUS       DASREK010000210         5188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1089559, whole genome shotgun sequence.
ACCESSION   DASREK010000210 DASREK010000000
VERSION     DASREK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089559"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1037)
                     /locus_tag="VFA54_10960"
     CDS             complement(9..1037)
                     /locus_tag="VFA54_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZS51373.1"
                     /translation="MNPTAALVFTIVFVALAFDYVNGFHDAANSIATIVATRVLSPAR
                     AVAWAAFFNFIAAFGFGTAVAQTVGNGLIRPGVVNEHVILGGLAGAIIWDVLTWYFGL
                     PTSSSHALVGGYAGAAMARVAVQEGWRKIWSALIPAGWTKTLLGIVVAPVMGMLCAVV
                     LMFVVTWAFHKMQPRVLDHHFRRFQLFSAAIYSFAHGTNDAQKTMGIIAGALFTAGYV
                     KVFYIPTWVILAAHAAIALGTFTGGWRIVKTMGARLTKLKPMGGFCAETAGAITILTA
                     ASFGGLVSTTHTITGAIVGVGTIHRVRAVRWGLATSIIWAWIFTIPAAAAIAGVAYWA
                     IRSIVPIS"
     gene            complement(1056..1670)
                     /locus_tag="VFA54_10965"
     CDS             complement(1056..1670)
                     /locus_tag="VFA54_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZS51374.1"
                     /translation="MRVLPKEEKFYTLFEQQAQFAAEAAQLLTAAVVGDLSQLGSYVA
                     QIRDVEHKGDEVTHEIMTRLNQTFLTPIDPEDIHRLASCLDDVLDMIDAAVGRLLLFK
                     LKSLPPEVAALAQIVEACTRAIVKAIGAMAHDEPVNDHLIEVNRLENEADRVNRQALA
                     DLFERETNAIELIKLKEIYEILEAATDLCEDVANVIEGVVVKNA"
     gene            1774..2235
                     /gene="bcp"
                     /locus_tag="VFA54_10970"
     CDS             1774..2235
                     /gene="bcp"
                     /locus_tag="VFA54_10970"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HZS51375.1"
                     /translation="MLQIGDNAPDFTATADSGEKIRLKDLRGKKVILYFYPKDDTPGC
                     TREACNFRDLYARFKKKNVVVLGVSVDSAASHAKFKSKYGLPFPLISDEDHKIVEAYG
                     VWKEKSMYGKKYMGTERTTFLIDEKGKIARIYEKVKVDGHADALLEAVSEK"
     gene            complement(2183..2986)
                     /locus_tag="VFA54_10975"
     CDS             complement(2183..2986)
                     /locus_tag="VFA54_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZS51376.1"
                     /translation="MSAAGRANELDGREWTRCSLSVWADIRLTAEERRLGHPAMFPTT
                     LVERLIQCFMREADRFILDPFLGSGSTLAAARNQGKVGAGFEVCEKFIRAARQRAGGD
                     FQIIRADARHLGEYVAAGSVDFCVTSPPYWNILQQKRTADHKRTRHYGAAENDLGKIA
                     EYGQFLDELGKVFAAVYTALRPGKYCVVNVMDLRKASRFYPLHADLAVRMQALGYVFD
                     DLIIWDRRMEYNNLRPLGYPHVFRINKVHEYLLIFQKPLPAAHRRARQP"
     gene            3124..3807
                     /locus_tag="VFA54_10980"
     CDS             3124..3807
                     /locus_tag="VFA54_10980"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HZS51377.1"
                     /translation="MNRLEQSSGPVSQRLAAGLAKIGLAIRNRSWKQAAGSGLTPTQG
                     QILAVLGLRRQPSTLAEVAGALGITAPTASDALQALQRKRLVRRVRSRVDGRSLALTL
                     TARGRKQADRCAGWPNFLAEAVDALPLAEQQALLRAVIQIIKSLQDRREIPVSRMCVT
                     CRYFRPNVHTDSQRPHHCDFVDAPFGERLLRLECPDHQPAAPDLEARNWQRFLLTGKA
                     ISSQEESIL"
     gene            3804..4397
                     /locus_tag="VFA54_10985"
     CDS             3804..4397
                     /locus_tag="VFA54_10985"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HZS51378.1"
                     /translation="MSQTAIPGYTYGTATVPHSPLTIQEFDLLKQAVLFTDEDVKYLR
                     MSRDVLADQTSAILDVWYGFVASHPHLVYFFGRKSDGQPDAHYLQAVRKRFEQWVLDT
                     AAAEYDQKWLDYQYEIGRRHHRSGKNKTDGVAAADNVSYRYIPALIYPITATLKPFLA
                     KKGHSPEDVEKMHQAWLKSVILQVILWSQPYVKEGDF"
     gene            4658..>5188
                     /locus_tag="VFA54_10990"
     CDS             4658..>5188
                     /locus_tag="VFA54_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZS51379.1"
                     /translation="MRDLVQRYLDSKLSRRGFLSSMTGLGFTAAAAQAVLAPLEASER
                     AAARTDIHGASMMEGTGGDLVIAQAKAAGAEYMFCNPGSFEVGLYDAFTDTPGMHLIM
                     GLHEGVVISMADGYHRVTGRPAFVNVHVIAGTAQAGGQIYNASRDGSALVITAGLNDN
                     ENWSDEAGLAPRPGFDQ"
BASE COUNT         1067 a         1544 c         1548 g         1029 t
ORIGIN      
        1 gttcccgctc agctgatagg cacaatcgac cggatggccc agtaagccac gccggcgatg
       61 gctgcggccg ccggtatcgt gaaaatccag gcccagataa tgctcgtcgc cagaccccag
      121 cgcaccgcgc ggacgcgatg gatggttccc actcccacaa tggctccggt gatcgtgtgc
      181 gtggttgaga ccaggccgcc gaaagatgcg gcggtcagaa tcgtgatcgc cccggccgtt
      241 tctgcgcaga aaccgcccat gggttttaat ttcgtgagcc gggcccccat ggtcttgacg
      301 atgcgccagc cgccggtgaa ggtgcccagc gcaatggcgg cgtgagcggc caggatgacc
      361 caggtgggga tatagaagac cttcacgtag ccggcggtaa ataaggctcc ggcgatgatg
      421 cccattgttt tctgcgcgtc gttggtcccg tgcgcgaaac tgtaaatcgc cgcggaaaag
      481 agctggaacc ggcggaagtg gtgatccagc acccgcggct gcatcttgtg gaaggcccag
      541 gtcacgacaa acatcagcac tacggcgcag agcatcccca ttaccggggc cacgacgatt
      601 cccagcagcg ttttggtcca gcccgcgggg atcagcgccg accatatctt gcgccacccc
      661 tcctgcaccg cgacgcgcgc catcgcggcg cccgcgtagc ccccgaccag cgcatgagaa
      721 gagctggttg gcagcccgaa ataccaggtc agaacgtccc agataatcgc tccggccaag
      781 ccacccagaa tgacgtgttc attgaccacg cccggccgga tcaaaccgtt tcccacggtc
      841 tgcgccacgg cggtgccgaa tccgaaagcg gcgatgaaat tgaagaaagc cgcccacgcg
      901 acggcgcggg cgggtgaaag gacgcgagtg gctacaatcg tcgcgatgga attggctgca
      961 tcgtggaatc cattgacata gtcaaaggcc agcgcgacga aaacaatcgt gaaaaccagg
     1021 gcggctgtcg gattcatcag cgcgaggcaa gggcttcagg cattcttgac caccacgccc
     1081 tcaatcacat tcgcgacatc ctcgcacagg tccgtcgccg cttccaggat ttcataaatc
     1141 tccttcagct tgataagttc aatcgcgttg gtttctcttt cgaacaggtc ggccagagcc
     1201 tgccggttca cgcggtcggc ttcgttttcc aggcggttga cctcgatcag gtggtcgttg
     1261 accggctcgt cgtgagccat agccccgatc gctttgacga tggcccgtgt gcacgcttcc
     1321 acgatctgcg ccagcgccgc gacctcgggc ggcagactct tgagcttgaa cagcagcagc
     1381 ctgcccaccg cggcatcgat catgtcgagc acgtcgtcca ggcaggatgc caggcggtgt
     1441 atgtcttccg ggtcgatcgg ggtaagaaac gtttgattca ggcgggtcat gatctcgtgg
     1501 gtaacctcgt cccctttatg ctccacgtca cgtatctgcg ccacgtaaga accaagttgg
     1561 gaaaggtcgc ccaccacagc cgcagtcagc agttgcgccg cctcggccgc gaattgagcc
     1621 tgctgctcaa acaaggtgta gaacttttct tcctttggga gaactctcat gaactgactt
     1681 gagcggttcg gaaattcatc ttctcagatc cacgacatcg ggtcaacggc aggttaagat
     1741 ggatctcttc tatcattgcc aggggggcgt ggaatgttac agattggtga caatgcgccg
     1801 gactttaccg ccacggccga ttccggcgag aagatccgtc ttaaggatct gcgcgggaaa
     1861 aaagtaatcc tctactttta ccccaaggat gacactccgg gctgtacccg ggaggcttgc
     1921 aattttcggg atttgtacgc ccggttcaaa aagaaaaacg tcgtcgtgct gggggttagc
     1981 gtggatagtg cagcctctca cgctaagttc aaatcgaagt acggactgcc gttccccttg
     2041 atcagcgatg aggatcacaa gattgtcgaa gcctacggcg tgtggaagga gaaatcgatg
     2101 tacgggaaaa aatatatggg cacggagcgc accacgttcc tcattgacga aaaggggaag
     2161 attgcccgga tttatgaaaa agtcaaggtt gacgggcacg ccgatgcgct gctggaagcg
     2221 gtttctgaaa aatgagcagg tactcgtgaa ccttgttgat gcggaagacg tgcggatagc
     2281 caagcggccg aagattgttg tactccatac gccggtccca gataatgaga tcgtcgaaaa
     2341 catatccgag cgcctgcatc cgcacggcca ggtccgcgtg tagaggatag aagcggctgg
     2401 cttttctcag gtccatcaca ttcaccacgc aatactttcc cgggcggagc gccgtataca
     2461 ctgcggcgaa gactttgccc agttcatcga ggaactggcc gtactcggca atcttcccga
     2521 gatcgttctc cgctgcgccg tagtgccgcg tccgcttatg gtccgccgtg cgcttctgtt
     2581 gcagtatgtt ccagtaggga ggcgaggtca cgcaaaaatc cacggaaccg gccgccacgt
     2641 attcgcccaa atggcgcgca tcggcgcgaa taatttgaaa atcgccgccg gctctttgac
     2701 gcgctgcgcg gatgaatttt tcgcaaactt cgaagccggc gccgactttt ccttggttcc
     2761 gcgccgcggc cagggttgag ccgctgccga ggaacggatc gaggatgaag cggtccgcct
     2821 cgcgcataaa gcactggatg aggcgttcca ccagcgtggt ggggaacatt gccgggtggc
     2881 ccaaacgcct ctcctccgca gtcagccgga tatcggccca cacactgagg gagcagcggg
     2941 tccactcccg cccgtcaagc tcgttggcgc gcccggcagc gctcattaac ggaaaagata
     3001 gcaaaattgg aggcctccgc cgcgccggaa aagcgttcct tcgcgcgctg cggagggcgc
     3061 cgggccgccc gcggggttga cacgaagcgc gtgtggcgat atatttagga gtcctaatat
     3121 attatgaacc gtctcgagca gagcagcggg cccgtctccc aacggctcgc cgccggcctc
     3181 gccaaaatcg ggttggcgat tcgaaaccgc tcctggaagc aggcggccgg gagcgggctt
     3241 accccgacgc aaggtcagat cctggcggtg ctggggctgc gccgccagcc ttctactctg
     3301 gcggaggtcg ccggcgcgct gggaattacc gcgccgacgg ccagcgacgc gttgcaggcc
     3361 ctacagcgca agcggcttgt gcgaagagtc cggtcgcgcg tggacgggcg ttctctggcc
     3421 ctcaccctga cggccagagg gagaaagcag gccgatcgct gcgccggctg gcctaatttc
     3481 ctggcggagg ctgtggatgc ccttccgctc gccgaacagc aagcgctcct gcgggcggtg
     3541 attcagatca tcaagtcctt gcaagaccgg cgcgagattc ccgtttcacg tatgtgcgtc
     3601 acgtgccgct attttcggcc gaatgttcac acggattccc aacggccgca ccactgcgat
     3661 tttgtcgatg cccctttcgg cgagcggctt ctgcgcctgg aatgtcccga tcaccagccc
     3721 gcagccccgg atttagaggc gcgcaactgg cagcggtttt tactaactgg caaggccatt
     3781 tcatcacagg aggaaagtat tttatgagtc agacggctat acctggttat acctatggca
     3841 ccgcgacagt gccccactcg cccctgacta tccaggaatt cgatctgttg aaacaggcgg
     3901 ttctcttcac cgacgaagat gtgaaatatc tgcgcatgtc gcgagacgtg ctcgccgatc
     3961 aaaccagcgc catcctggat gtatggtatg gattcgtcgc ctcccacccg catctggtgt
     4021 attttttcgg aagaaagtct gacggccagc cggatgccca ctacttgcag gccgttcgca
     4081 aacgcttcga gcagtgggtc ctcgatactg cggcggcgga gtacgatcag aagtggctgg
     4141 attatcaata tgagatcggc cggaggcacc accggagcgg caagaacaaa acggacggcg
     4201 tggccgcggc cgataacgtg agctaccgct acatcccggc cctcatttac ccgattacgg
     4261 ccacgttgaa accatttctg gcaaaaaagg gccacagccc cgaagatgtc gagaaaatgc
     4321 accaggcttg gctcaaatcg gtgattcttc aggtgattct ctggagccag ccttacgtca
     4381 aagaaggcga tttctagttc tcccgcgctg tggaggcgtc cgagctggcg gttcgcctcc
     4441 gcggctgttc gacttttcgc tgaaaaacct cacaatcaga tattttttct ccccgtgcgc
     4501 gctccgcttc tgcaacggca tccataaaca gctcgcaggc cgctggaaat cttgcgtgga
     4561 atccggcgat cttcccggca gagcttgaat agcctgccgg ctggcgttat catctcgaca
     4621 gtcaattttt gagccgctgg ctctccgagg aggcgccatg agagatctcg tacaacgcta
     4681 tctggattcc aagctttccc ggcggggttt tctgtcgagc atgacgggat tgggcttcac
     4741 cgccgctgcc gcacaagccg ttctggcgcc gctcgaggcg tcagaacggg cggctgcacg
     4801 aactgatatt cacggagcct cgatgatgga gggtacgggc ggcgatctag taatcgccca
     4861 ggctaaggcg gccggcgccg agtacatgtt ctgcaatccc ggctcgttcg aggttggcct
     4921 gtatgatgcg ttcaccgaca cgccgggcat gcacctcatc atgggtctcc atgaaggagt
     4981 cgtcatctcg atggccgatg gctaccatcg cgtgacgggc cgtcctgctt tcgtaaacgt
     5041 tcacgtcata gccggaacgg cgcaagccgg gggccagatt tacaatgcca gccgcgacgg
     5101 ctcggcgctg gtgatcacgg ccggcctcaa cgataacgag aactggagcg atgaggcggg
     5161 tcttgcgccg cgcccgggct tcgatcag
//
LOCUS       DASREK010000211        10834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_595824, whole genome shotgun sequence.
ACCESSION   DASREK010000211 DASREK010000000
VERSION     DASREK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595824"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFA54_10995"
     CDS             complement(<1..224)
                     /locus_tag="VFA54_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZS51380.1"
                     /translation="MIIDSHCHLDDPKFDHDRPEVLERARQAGVALALAIGSGDGPPD
                     LEPAIRLAKTVPWLYATAGIHPHEARKADAA"
     gene            complement(253..2241)
                     /gene="metG"
                     /locus_tag="VFA54_11000"
     CDS             complement(253..2241)
                     /gene="metG"
                     /locus_tag="VFA54_11000"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZS51381.1"
                     /translation="MKYFLTTPIYYVNAAPHVGHAYCTIVADAVKRFRRMKGDEPFLV
                     TGTDEHGQHVERAVKDSGFTPQQFTDRVSAAWRAQWEELGLAYDSFVRTTSPQHHRTV
                     QWLFERCRNNGYIYKGQYTGQYCVNCELYVNDAAPGDPCPNCGKTTETVTEENLFFKL
                     SEFESRLLKLYEDNPAFILPESRRNEVLSFVRAGLRDLSISRTSITWGIPVPGEPRHV
                     FYVWFDALSAYMTGAGYGGDESQTARAERLWPADLHLIGKEIVRFHAVYWPAFLMAAN
                     LPLPKRIFAHGWLLFQEEKMSKSRGNVVRPSPIAKVVGRDGLRYYLLRDIVFGQDGSF
                     SYDALATRYNSDLANGLGNLASRTLAMIRDYREGTIPGSAEALMTVAPGGSGETRRID
                     EIAQETIASVRAAFENFEFSKGLEQLWSFISTVDRFLVQHAPWQLAKNPAERPHLDST
                     LYAAAEALRLCAVLLYPVLPESTEKIWRQLGMSGALAGQAVDQLAWGDLTPGQKIGKV
                     EAVFPRIDKAEAIQKMQELEAQTVKEQAALLGKQEASEPISPASDGKISIDDFAKIDL
                     RVGVVKSAERVKGADKLLHMMVDIGEPQPRSIVAGIAPAYSPEQVIGRKVVIVANLQP
                     RKLRGIESNGMIVAASLEGGAPVLAGFLEDVPVGARLK"
     gene            complement(2290..3474)
                     /gene="larC"
                     /locus_tag="VFA54_11005"
     CDS             complement(2290..3474)
                     /gene="larC"
                     /locus_tag="VFA54_11005"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HZS51382.1"
                     /translation="MKICYFDCFSGVSGDMVLGALADAGADRAELSRQISRLGLEAVQ
                     VAFEPCARGGIAATKFRVQAPEGRQHRHLSQIERIIRAASLGPAVEERSIAIFRRLAE
                     AEAAIHQMPVEKVHFHEVGAADSIVDIVGACIALELLEADRIYCSPLNLGSGTVQCAH
                     GALPVPAPATAALVQGKPVYSRGPAVELTTPTGAAIVVTLAAGFGPMPPMCIGAIGYG
                     AGDRDFPEQPNLLRVMLGEAAGASEAVEIWVLEANIDDMSPQLAGYVTERLLEAGALD
                     VTWAPVYMKKDRPGFTLTALAQPQDRERLAALLFAETTTLGIRTYPAQRRVLDRSWTE
                     VETEFGKIRVKIARDGGRVRNFAPEYEDCRRAAREKNVPLKEVIQKVNFEFLKRSEAG
                     GL"
     gene            complement(3808..6288)
                     /locus_tag="VFA54_11010"
     CDS             complement(3808..6288)
                     /locus_tag="VFA54_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HZS51383.1"
                     /translation="MFERYTEKARRVIFFARYEASQFGSPYIETEHLLLGLLREDKAL
                     ANRFLRSHAAVESIRKQIEGHTTIREKVSTSVDLPLSHECKRVLAYAAEEAERLSHKH
                     IGTEHLLLGLLREDKCFAAEILHERGLRLSTIREELQRSQSEKLLSNRPKESSLLAEF
                     SRDLTQAAMDNTLDPLIGRESELERVVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQR
                     IADGEVPSFLADKRILALDLSLIVAGTKYRGQFEERLKTIMKELMENQNAIIFIDELH
                     TLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEYRKSIEKDRSLERRFQAVKVP
                     PPSEEDAVKVLFGIKDRYEKFHAVSYTDDSIQAAVYTSSRYIPDRFLPDKAIDLVDEA
                     GARVKLRQTTLPDELADIQKRIKFIVHRMENAIANHEFEKARFYSDEERKERENLRLL
                     REKYNLDESSIGVVTKEDIEEVVARWTGVPMTAIKEEEMAKLLRIEEELHKRVISQDK
                     AISALARAIRRSRAGLKSVTRPAGSFLFLGPTGVGKTEVARALAQFLFGSEKSLIRFD
                     MSEFMEKHSVSKLIGSPPGYVGYEEGGQLTERVKRAPYSIILLDEIEKAHPDVFNILL
                     QVFEDGHLTDGLGNTVDFKNTIIIMTSNLGARFLEKRSHLGFQSASLEATAAKMEDLV
                     KSELKRVFNPEFLNRLDEVILFTSLTDEDLIKIIGLLVEQINANLAGKQIKIRLAEDA
                     AKYILEKTCGDRGYGARPLRRALQKYIEDPLSEALIQGNLPRPAELEVYLGENGMYYR
                     LVKEAKVMADGGQEESTETGAGVPLYMF"
     gene            complement(6355..7035)
                     /locus_tag="VFA54_11015"
     CDS             complement(6355..7035)
                     /locus_tag="VFA54_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51384.1"
                     /translation="MPVIRTVHLARIYRSGAVDLPVFTDVNLAIERGEQVAIVGESGA
                     GKSTLLHLLGALDRPSSGTIYFESRNLLELTERELAEFRNREVGFVWQIHYLLPEFTA
                     LENVVMPLLIRGQGRREAEVEASKWLAEVGLAGRASHRSGELSGGEQQRVALARALVG
                     RPSLLLADEPTGNLDLRNGEMIMDLIGDLHRSHQLTSVIVTHNLTFARKCDRVLRLEA
                     GRLIEEPD"
     gene            complement(7039..7566)
                     /locus_tag="VFA54_11020"
     CDS             complement(7039..7566)
                     /locus_tag="VFA54_11020"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZS51385.1"
                     /translation="MHLKLKDSPIIVLVGFMGCGKSTVGRLLAERLGWQFVDLDEEIE
                     RQAGSPIVEIFEREGERKFRELEHAALVEQLRLAQRGRARVLALGGGAFAESKNRERL
                     EAGGVAIWLDCPVETLWERVSQGAGRPLARRREEFEQLYRERLPHYRLADYALRSDVL
                     EPEAVVDAILQLLLF"
     gene            complement(7694..8527)
                     /locus_tag="VFA54_11025"
     CDS             complement(7694..8527)
                     /locus_tag="VFA54_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS51386.1"
                     /translation="MDLRQKVVLITGASMGIGEACAYAFARRGARLILTARSAGSLER
                     VRQAAAPADAAALAADLSQPDQAQALVPRALGCYGRIDVLVNNAGVGLYTPFWEADRN
                     AIRLLMEVNFFAPLELIRGVLPHMRRQGVGTIVNVSSVAGKIPLPWLTLYSASKAALN
                     YISDGLRMELHGTSINVVSVCPGYVSTGFPQHVLGGAIPETVARRKRFTITAEQCAEA
                     IVKGVERGKRTVVTPASGWLLIGISRLLPGPAHALLAHMRRPAQTVDQVNEESGSRSQ
                     R"
     gene            8582..9202
                     /locus_tag="VFA54_11030"
     CDS             8582..9202
                     /locus_tag="VFA54_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZS51387.1"
                     /translation="MVSDLLLPLFPLEVVLLPQATLPLHIFESRYKLMIGEALEQQSE
                     FGVVLFKQNALVNIGCTAAIERVVRKYPDGRLDIITAGRRRFEILFLNQEKPYLQAGV
                     HFFADDAGTAPTEAAASRLQELFQRVQAFLPPGARLPGSPGRGRDSFQIAGALPLDLD
                     FKQRLLALRSEAERVALLSEYLEKLAPRLQLTKRVERSAQSNGHGR"
     gene            9503..10222
                     /locus_tag="VFA54_11035"
     CDS             9503..10222
                     /locus_tag="VFA54_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HZS51388.1"
                     /translation="MLSFIMRCSSRNAKGPAAVVGGACEKAGAVHISAYLTSGLMVLA
                     AACGGSIPKTHYYVLNLPPPAPRAGDPSPYTAVVMPVRAPEQLEQDRIVYSPSPVEIE
                     FYEYHRWAQRPAETLTNAIAERLRAARLFSNVIVFDGRTKGDYLIRSRLNRLEEVDSE
                     NGVAVTVELAADAVEAKNNRVVWTSSASHTGPVTSGDVRAVVTEMSRGVDACLGKITA
                     DLENLRKAFPPPPAPASIASP"
     gene            complement(10149..>10834)
                     /locus_tag="VFA54_11040"
     CDS             complement(10149..>10834)
                     /locus_tag="VFA54_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZS51389.1"
                     /translation="NLTITDLHGLTIKLDEAGPELSREEERAIEEAVRKELDSAQWLL
                     LCGSLPPGLSVDFYARLIDMARRRGVKTLLDTGGEALRVGLQARPTIAKPNRPEAERL
                     LDRALLTRQQSIEALHEILQMGPESVVLSLGSEGALGTAGNGTYHALAPQIRQACPIG
                     AGDALAATCVWALSQNQPFPEALRWGVAAGTAAAATPGTSFASFRETQWMREQVEVEM
                     LSEGSPSLP"
BASE COUNT         2057 a         3393 c         3251 g         2133 t
ORIGIN      
        1 gccgcatcgg ccttccgggc ctcatggggg tgaatcccgg ctgtggcgta cagccacggc
       61 acagttttcg ccaagcggat cgcgggttcg aggtcgggcg gtccgtctcc cgagccgatc
      121 gccagcgcga gagccacgcc ggcctgccgc gcccgctcga gcacttcagg ccgatcatga
      181 tcaaacttcg gatcatcgag atggcagtgg gaatcgatga tcaccggagc gaggtccttg
      241 cgaaggcccg gctcacttca gcctggcccc gacgggcacg tcttccagaa aaccggccag
      301 aaccggggcg ccgccttcga gggaggcggc gacaatcatg ccattggact cgatgcctcg
      361 cagcttgcgc ggctgtaagt tggcgacgat cactaccttg cggccgatga cttgctcggg
      421 ggagtaggcc ggggcgatgc ctgcgacgat cgagcgcggc tgtggctcgc cgatatcgac
      481 catcatatgc aggagcttat cggcgccctt gacgcgctcg gcggacttca cgacgcctac
      541 gcgcaggtcg atcttggcga aatcgtcgat cgagattttc ccgtcagaag ccggtgaaat
      601 tggctccgaa gcctcttgtt tgcccagcag cgccgcctgc tccttcaccg tttgtgcttc
      661 gagctcctgc attttctgaa tagcctccgc cttgtcaatt ctcgggaaaa cggcttccac
      721 tttgccgatc ttctggcccg gagtgagatc tccccaggca agctgatcta ccgcctgtcc
      781 ggccagcgcc ccgctcatgc cgagctgccg ccagatcttc tcagtggatt ccggcagcac
      841 cgggtaaagc agcacggcgc acagacgcag agcctccgcg gcggcgtaga gcgtggaatc
      901 caggtgtggc cgttccgccg ggttctttgc cagttgccac ggcgcgtgct gtaccagaaa
      961 gcgatccact gtcgaaataa aagaccacag ctgttccaag cccttcgaga attcgaaatt
     1021 ttcaaaggca gcccgtacag aagctatggt ttcctgcgct atctcatcaa tccgcctggt
     1081 ctcgcccgag ccgccggggg cgacggtcat cagcgcctcg gcgctgccgg gaatcgtccc
     1141 ctcgcggtaa tcgcggatca tcgccaaagt ccgactggcc agattgccga gcccgtttgc
     1201 cagatccgag ttgtaccgag tcgccagcgc gtcatagcta aagctgccgt cctgtccgaa
     1261 gacaatgtcg cgcagcagat aatagcgcag cccgtccctg cctactacct tcgctatcgg
     1321 cgaggggcgc actacgttgc cgcgcgattt gctcatcttc tcctcctgaa acaggagcca
     1381 gccatgggcg aaaatgcgct tcggcagagg cagattggcg gccatgagaa acgccggcca
     1441 atagacggcg tgaaaccgga cgatttcctt cccgatcaaa tgcaggtcgg cgggccaaag
     1501 gcgctcggcc cgggcggtct gtgattcatc gccgccgtaa ccggcgcctg tcatgtatgc
     1561 gctcagggcg tcaaaccaga cgtagaaaac gtgtctgggc tcgccgggga cagggattcc
     1621 ccaggtgatc gacgtgcggc tgatggagag gtcgcgcaag ccagcccgga cgaacgagag
     1681 cacctcattg cggcgggatt ccggcaggat aaacgccgga ttgtcttcat agagcttcag
     1741 cagccggctc tcgaactccg agagcttgaa gaatagattt tcttcggtaa cagtctcggt
     1801 tgtctttcca cagttagggc agggatcgcc gggagccgcg tcgttcacgt acagttcgca
     1861 attgacgcaa tattggcccg tatattggcc tttgtaaatg tagccgttgt tgcggcagcg
     1921 ctcgaaaagc cactgcactg tgcgatggtg ctgcgggctg gtcgtgcgga caaagctgtc
     1981 gtaggccagg ccgagctcct cccattgcgc gcgccaggcc gccgaaaccc ggtcggtgaa
     2041 ttgctgggga gtgaatcccg aatccttcac ggcgcgctcg acgtgctgcc cgtgctcgtc
     2101 ggtgccggtc accagaaagg gctcatcgcc cttcatgcgg cggaaccgct tgaccgcatc
     2161 agcgacgatg gtgcaatagg catggccaac gtgcggcgcg gcattgacat aatagatcgg
     2221 cgtggtcagg aaatatttca tggttgaaag cggccgtacg ctggccgccg gatgagccag
     2281 gccgctttcc tacaggccgc cagcctcgct cctcttcaaa aactcgaaat tgaccttctg
     2341 gatcacttct ttcaatggca cattcttctc gcgagccgcc cggcggcaat cttcgtactc
     2401 cggagcgaaa ttgcgaacgc ggccaccgtc ccgtgcgatt tttacgcgga ttttgccaaa
     2461 ttccgtttcc acctcggtcc aactgcgatc gaggactcgc cgctgcgccg gatacgtgcg
     2521 gatcccgagc gtagttgtct cagcgaaaag aagcgcagcc agccgctcgc ggtcctgtgg
     2581 ctgcgccagc gccgtgaggg tgaagccagg gcggtctttt ttcatataca ccggagccca
     2641 agttacatcg agtgcgcccg cttcgagcaa acgctcggtt acgtagccgg cgagttgggg
     2701 actcatatcg tcgatattgg cttccagcac ccaaatctcg acggcttccg acgcgcccgc
     2761 ggcttccccc agcattaccc gcagcagatt cggctgctca ggaaagtcgc gatcgcccgc
     2821 cccatagccg atagcgccga tgcacatagg cggcattggc ccgaaaccag ccgccagggt
     2881 gaccacgatg gccgcccccg tcggcgtagt cagctccaca gccggccccc gggaatatac
     2941 cggcttaccc tgtacgagag ccgccgtggc gggtgcggga accggcagcg caccgtgtgc
     3001 gcactgtacc gtcccagatc ccaggttgag cggcgagcaa taaatgcggt cggcctcgag
     3061 cagctccaag gcgatgcacg cgccgacaat gtcgacgatg gaatcggcgg cgcccacttc
     3121 gtgaaagtga actttttcga ccggcatctg gtgaattgcc gcctccgctt ccgcaagccg
     3181 gcgaaaaatc gcaatactgc gctcttccac ggcaggcccc aggctcgccg cgcgaatgat
     3241 cctttcgatc tgggacaggt gccggtgctg gcggccttcc ggggcctgga cgcgaaactt
     3301 ggtcgccgca atgccgccgc gcgcgcaggg ctcaaatgct acctgaacgg cttcgaggcc
     3361 caaacggctg atctgtcgcg acagttccgc acgatccgcg ccggcatcgg ccagcgcgcc
     3421 cagaaccata tccccgctga cgccggagaa gcagtcgaag tagcagattt tcattcaggc
     3481 tgacttccat tatgcaacac aaccgaagcc gcggctttcg ccgagatcga gcgacgcccc
     3541 ggccgggcct tcagcgtctt atcataccgg ctgtaaggat gggccgatcc ccgcatccgc
     3601 actggccgcc ttgtcgcgcc cggaggcggg cttcgctgcg ctcagagtaa tagttggcag
     3661 ccggttcggc cgaaggacct acgctctcgc gaaaacaaaa gggcaagtcg gttgtttttc
     3721 ccggcatgga cacgccgacg cccgtggccc gctcagaatg gcaaggttgg agattctccg
     3781 ctcaatgacc gaagggcgat tccggcgtca gaacatatac aggggaacgc cggcgccggt
     3841 ctcggtgctt tcttcctggc cgccgtcagc catgactttg gcttccttca ccaggcggta
     3901 gtacatcccg ttttcgccga gatacacttc cagttcggcc ggccgcggca gattgccctg
     3961 gatcagagct tccgacagcg ggtcttcaat gtatttctgc aacgcgcgcc gcaggggacg
     4021 cgctccatag ccccggtcgc cgcaagtctt ttcgagaatg tatttagcgg cgtcctcggc
     4081 gagccggatt ttgatttgtt tgcccgccag attggcgttg atttgctcca ccagcaggcc
     4141 aatgattttg atcaggtctt cgtcggtgag cgacgtgaac aagatcactt catccaggcg
     4201 gttgaggaac tccggattga aaacccgctt cagctcgctc tttaccagat cctccatctt
     4261 ggcggcggtg gcttccagtg acgccgattg gaaccccagg tgagagcgtt tctccaggaa
     4321 ccgggcgccc aggttcgagg tcatgataat aatcgtgttt ttgaaatcaa cggtattgcc
     4381 aagaccatcg gtgagatggc catcctcgaa aacctgaagc aggatattga atacgtccgg
     4441 atgggccttt tcaatttcat ccaaaaggat gattgaataa ggcgcgcgct tcacgcgttc
     4501 ggttagctgg ccgccctctt catagcccac gtaacccgga ggcgacccaa tcagcttgga
     4561 gaccgagtgt ttctccataa actcggacat atcgaaccgg atgagcgatt tctcgctgcc
     4621 gaacaggaat tgggcaagcg cccgggcgac ttccgttttt cccactccgg taggtcccag
     4681 gaacaggaag gatccggccg gtcgggtcac cgatttcagc cccgcgcggg aacggcggat
     4741 ggcccgggcc agagcggaaa tcgccttgtc ctggctgatt acgcgcttgt gcagctcctc
     4801 ttcaatgcgc agcaacttgg ccatttcctc ttccttgatg gccgtcatgg gaacgccggt
     4861 ccaccgggcg acgacttcct cgatgtcctc tttggtcacc acgccgatcg agctttcatc
     4921 aaggttgtac ttctcccgca gcaggcgcag gttttcgcgc tccttgcgtt cctcatcgga
     4981 gtagaagcgc gccttttcga actcgtggtt ggcaatggcg ttttccatcc ggtgaacgat
     5041 gaacttgatc cgcttctgaa tgtctgccag ctcgtcgggc agcgtggtct gccgcagttt
     5101 tacgcgagcg ccggcttcgt ccactaaatc gatggcttta tcgggcagga agcggtcggg
     5161 gatgtaccgg ctggaagtgt agaccgcagc ctgaatggag tcatccgtgt agctgacggc
     5221 gtggaatttt tcgtagcggt ctttgatgcc gaacaggacc tttacggcgt cttcctcgct
     5281 cggaggggga actttaacgg cctgaaaccg gcgctcgaga gaacgatcct tctcgatgga
     5341 tttccggtac tcagccggcg tggtcgcccc gatgcactgg atttcgccgc gggagagagc
     5401 cggcttcaag atattggcgg cgtcgagaga gccttccgcc gaccccgctc cgaccagagt
     5461 gtgcagctcg tcgatgaaga tgatggcgtt ctggttttcc atcagctcct tcatgatggt
     5521 cttgagccgc tcttcaaatt gtccgcggta tttggttccg gcgacgatga gagacaggtc
     5581 gagcgccagg atgcgcttgt ccgccaaaaa cgacggcacc tcgccgtcgg cgatgcgctg
     5641 ggccagacct tccacgatag ccgtcttgcc cacgccgggt tcgccgatga gcaccggatt
     5701 gtttttcgtg cggcggcaaa ggatttgtac tacgcgctcc agttcgctct ctctgccgat
     5761 cagcgggtcg agcgtgttat ccatcgccgc ttgcgtcaag tcgcggctga actcggccag
     5821 gagcgagctt tccttcgggc ggttggaaag cagtttctcc gattgcgacc gttggagctc
     5881 ctcgcggatg gtggagagcc gcaggccgcg ctcatgcaga atttcggctg cgaagcactt
     5941 atcctcgcgc agcagcccga gcagcaggtg ctcggtgccg atgtgcttgt gcgagagcct
     6001 ttccgcttct tcggcggcgt acgcgagcac gcgcttgcat tcgtggctga gaggcagatc
     6061 gaccgaagtg gagaccttct cgcggatcgt ggtgtggcct tcgatctgct ttcgaatcga
     6121 ctccacagcg gcgtgcgatc gcaaaaaccg gttcgccagc gctttatcct cgcgcagcaa
     6181 accgagcaga agatgctctg tctcgatgta ggggctgccg aattggctgg cctcgtaacg
     6241 ggcgaaaaag atcaccctgc gcgccttttc ggtatatcgt tcaaacataa aaccctggtc
     6301 tcccaaaact ttgagcgcca gccaagcgat cctgtggctt cgcgcacact cctgttagtc
     6361 cggctcctcg ataagcctcc cggcctccaa acgtaacacc cgatcacatt ttcgcgcgaa
     6421 agtcaggttg tgggtgacaa tgacggaggt tagctggtgg gaacggtgca aatctccgat
     6481 caggtccatg atcatttcgc cgttcctcaa gtcgaggttt ccggtcggct cgtcggccag
     6541 caaaagactg gggcggccca ccagcgctcg agcaagagcg acgcgctgct gctccccgcc
     6601 ggaaagctct ccggaccggt gcgaggccct gccggccaat cccacttccg ccagccattt
     6661 tgaagcctca acttcagcct cccggcgacc ttggcccctg atcagcaggg gcatgaccac
     6721 gttctcaagc gccgtaaact ccggcagcag ataatgaatc tgccacacga aaccaacttc
     6781 cctgttccgg aattccgcta attcccgttc cgtcagttct agtaggttcc ttgattcaaa
     6841 gtatatcgta ccgctgcttg gcctgtctag cgcgcccagc aaatgcagca gcgtggactt
     6901 gcccgcgccg gattcgccta caatggccac ttgctcgccg cgctcgatcg ccagatttac
     6961 atcggtgaac acggggagat ccaccgcgcc ggagcgatat atgcgcgcca aatggacggt
     7021 gcggatgact ggcatgggtt aaaagagcag caattgcaga atggcgtcca ccaccgcctc
     7081 cggctccagg acgtcggacc gcagcgcata atcggccagc cggtagtggg gcaagcgctc
     7141 gcggtagagc tgctcgaatt cctcgcggcg ccgcgcgagc ggccggccgg cgccctgcga
     7201 tacgcgctcc cacagtgttt cgaccgggca gtccagccat atcgcgacgc cgcccgcctc
     7261 caagcgctcg cggttcttgc tctcggcaaa tgccccgccc cccaaagcca gcactcgcgc
     7321 gcggccccgc tgcgcgaggc gcaattgctc caccaaagcg gcatgctcga gctcccggaa
     7381 tttccgttcc ccttcacgct cgaagatttc gacgataggc gagccggctt ggcgctcgat
     7441 ttcctcgtcc agatccacga attgccagcc cagccgctcg gcaagcagcc ggcccaccgt
     7501 ggacttgccg cagcccatga agccgaccag aacaatgatc ggggagtcct ttagtttgag
     7561 atgcatgtat tcaagaaagt cagagggcag atgggagaag ccgcaacccg gaggatgcgc
     7621 ttgggttgct tccgagcgcc gttcgcgctc cgagccggca ctcggagtag acggaaggcc
     7681 gggccggcgg acttcaccgt tgacttcggc tcccggattc ctcgttcacc tggtccacgg
     7741 tctgcgcggg ccgccgcatg tgcgccagca gagcgtgagc cggcccgggc agcagccgcg
     7801 aaatgccaat caacagccat ccacttgccg gcgtgaccac ggtgcgcttt cccctttcca
     7861 cgcccttcac gatagcttcg gcgcattgct cggcagtgat cgtgaaccgc ttccttctgg
     7921 ccactgtttc cggtattgcg cctcccagaa catgttgggg gaagccagtc gaaacatagc
     7981 ctggacagac ggacaccaca ttgatgcttg tgccgtgcaa ctccatgcgc aggccatcag
     8041 aaatataatt cagagccgct ttgcttgccg agtagagggt cagccaaggc aaaggaattt
     8101 ttccggcgac ggagctgaca ttgacgattg ttcccacgcc ctgccggcgc atatgcggca
     8161 acacgccgcg aatcagttcc agcggcgcaa agaaattcac ctccatcaaa agccggatcg
     8221 cgttgcggtc cgcctcccaa aaaggagtgt acaggccgac gccggcattg ttgaccaaca
     8281 catcaatgcg gccgtaacag ccgagcgcgc gaggcacaag ggcctgggcc tgatccggct
     8341 ggctcaaatc cgcggccaga gcggccgcgt cggcaggagc ggcggcctgg cggacccgtt
     8401 cgagcgagcc ggccgagcgc gccgtcagaa taaggcgcgc gccccgccgc gcgaaggcat
     8461 aagcacacgc ctcgccgatg cccatcgaag caccggtaat gagcactacc ttctgtcgta
     8521 aatccactga tctggcccgt ccccacgttg cccgtaaccc tgcgggaagc cataatgtag
     8581 catggtctcg gacttgctgc ttccgctgtt ccctctggaa gtggtgctgc ttccccaggc
     8641 gactctgccc ctgcatatct tcgagagccg atacaagctg atgatcgggg aagccctcga
     8701 acaacagtct gaattcggag ttgtgctgtt caaacagaac gctctggtca acattggatg
     8761 cactgccgcg attgaaagag tggtgcggaa ataccccgat gggcggctgg atatcattac
     8821 ggccggccgg cggcgcttcg aaattctctt tctcaaccag gagaagccct atttgcaggc
     8881 cggcgtgcat ttctttgcag acgacgcggg gacggccccg accgaggccg cggctagccg
     8941 gttgcaggag ctgttccagc gcgtccaggc ctttttgccg cccggagccc ggctgcccgg
     9001 ctcgcccggc cgcggccgtg acagtttcca gatcgccggc gcgcttccac tggatctcga
     9061 tttcaagcag cggttgctgg ctctgcgttc cgaagcggaa cgggtggcgc tgctgagcga
     9121 gtatctggag aaacttgcgc caaggctcca attgaccaag agagtcgaga gatccgcgca
     9181 aagcaacggc cacgggcgct agcctagggt ctcgcacaaa gaacatcggc cttccggcgt
     9241 gcagcggtca ggccggatga tcccgccggc cggtggccca aaaaatcgcc gcaggaagcg
     9301 gcttggcctt tatactcaga atagtttctg ggctgctggg catggattgt gtttacagga
     9361 cggtaagatc gccagtggcg ggactgataa gaaatgggaa cgccggccca acacagcgcg
     9421 tagaaatcgc ggcgaaaaat tttcttcccc gtctcgaagg gcggtcggca gggcgcgaga
     9481 gcgccatgca agctcgccct tcatgttatc gttcataatg cgatgttcgt cgcgcaatgc
     9541 aaaaggcccg gcggcggtcg tcggcggggc ctgtgagaaa gcgggtgcgg tgcatatcag
     9601 cgcgtatttg acctccgggc tgatggtgct ggcggcagcc tgcgggggat cgattcccaa
     9661 aacgcattac tacgtgctca atcttccgcc gcctgcgcca cgggccggag acccgtctcc
     9721 ttacacggcg gtggtcatgc cggtgcgagc gccggaacag ctcgaacagg accggattgt
     9781 ttacagcccg tcgccggtgg aaatcgagtt ttatgaatac caccgctggg cgcagcggcc
     9841 cgctgaaacg ctcaccaatg cgatcgccga aaggctgcgc gccgcgcgtc tgttctccaa
     9901 cgtcattgtt ttcgacggca ggaccaaagg agactacctg atccgcagcc gcctgaaccg
     9961 gctggaggaa gtcgattccg aaaacggcgt ggcggtgacc gtcgaactgg ccgccgatgc
    10021 ggtggaggcc aaaaacaatc gtgtcgtctg gacgagctct gcgtcgcaca ccggcccggt
    10081 gaccagcgga gacgtccggg ctgtggtgac cgagatgagc cgcggagtgg acgcctgtct
    10141 gggaaaaatc acggcagact tggagaacct tcggaaagca tttccacctc cacctgctcc
    10201 cgcatccatt gcgtctccct gaacgaggca aaggacgtgc ccggcgtggc ggccgctgcg
    10261 gtgcctgccg caactcccca tcgcaaggct tccggaaaag gctgattttg cgacaaggcc
    10321 cacacgcagg tggccgccaa cgcgtcccct gcgccaatgg ggcaggcctg gcggatttgc
    10381 ggcgccagcg cgtgatacgt cccgtttccg gcagtgccca aagcaccttc gcttccgagg
    10441 gaaagcacga ccgattcggg tcccatctgc aaaatttcgt gcaaggcctc gatcgattgc
    10501 tggcgggtga gcagcgcgcg gtccaggagc ctctcggctt cgggccggtt cggttttgcg
    10561 atcgtcggcc gcgcctgaag cccaactcgc agagcctcgc ctccggtatc gagcaatgtc
    10621 tttacgcccc tccggcgggc catgtcgata agccgggcgt agaagtcaac gctgaggccg
    10681 ggcggcaggc tcccgcagag gaggagccat tgcgcgctat ccagctcctt gcgcacggcc
    10741 tcttcgatgg cgcgctcttc ttcccggctg agttctggac ccgcctcgtc cagcttgatc
    10801 gtcaggccat ggagatcggt gatcgtgaga ttgc
//
LOCUS       DASREK010000212         6415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_101901, whole genome shotgun sequence.
ACCESSION   DASREK010000212 DASREK010000000
VERSION     DASREK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101901"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFA54_11045"
     CDS             <1..550
                     /locus_tag="VFA54_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HZS51390.1"
                     /translation="FVQALLSGNALGVHDVESALHGTSLGVRLSDGRPEEHGHRNHMR
                     AINAIYHCGGIAQAVECGRLNSGILYQCVKSGVPFVLAGSLRDDGPLPDTITDMNAAQ
                     DRYQEQLRGAGLVLCLGSMLHSIATGNMLPSWVKIVCVDINPAVATKVSDRGTGQAVG
                     VVTDVGLFLDLLAKRLSPGWQK"
     gene            577..1776
                     /locus_tag="VFA54_11050"
     CDS             577..1776
                     /locus_tag="VFA54_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HZS51391.1"
                     /translation="MKNISRRDLGRLAAGAMSAGKLGSPGLGLAAAAAPQQPKSAYAG
                     ALTGVEPARLEDRRFDPVAYTHDLYAAAPRRLRFEAQNLRQAEAWQRKARARLIELIG
                     GFPSGRVPLRPVTLETREFPAYTREKVVFDSRKGVSVLAYVLMPRQPLRRPVPAVVCV
                     PGHGRGVDDIVGIDDQGRDRTNKDGYQHDFAIQVAEAGLAAVAIEQMAFGCRRDAITA
                     KKGLGQSACQPAAGGALLVGQTMIAWRVYDVMRTIDFIAARPELDSLRVGCMGISGGG
                     TCTLFASALEPRIRAAMVSGYLNTFRDSIGSLSHCIDNYVPGILNWFEMYDVAGLVAP
                     RPLFAESGEKDEIFPIRASLESFHRVKEIYSVFGVPGHVEQETFNGPHSFWGKKGIPF
                     LARHLAA"
     gene            1942..2610
                     /locus_tag="VFA54_11055"
     CDS             1942..2610
                     /locus_tag="VFA54_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51392.1"
                     /translation="MAVWDGFWENLSQLFSAIRDWFNPTPQMGVAFALWLLIAGLHYG
                     LMQVRPAAGKARPSRDLVWIDRLSRIVFPFATALLALFLLPESIVERLEFKAQSYLHQ
                     AGMLELHPLRWKLETGAVALLRFEGERIHVDVLSGRESRGGIRVAELTKQGRGYSGAA
                     QVGMPCPAGGPPHASCTKEVPIEFTLVQQRRIEGRLGLDPEKSQAACPHCRTQWKEFV
                     WVPE"
     gene            2794..5037
                     /locus_tag="VFA54_11060"
     CDS             2794..5037
                     /locus_tag="VFA54_11060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZS51393.1"
                     /translation="MQSRLLRVLYLCLILAGGLLAAIAMARQPGEPNAAAFAILLFLA
                     CGTAALKVQLPGVDRSVSLSFAFAFAAVTEFPAAHAFFVAAVSLVYAAFQEEKRAWQP
                     LDLAFNLASAAVSTLCTSEVYAHLGRDFESGRLIAMGTAAVVYYVLTSGTSALQIGFQ
                     TRTRPWKVWQEKFFWMGPLYLLVPVANGIAKMLRQTAWAPNRLLGLMLIFGGYRYVKH
                     YFGRLHDQQDHAKRLDGIRQRTIEALAVAIEAKNGAAAGHLQRVRRHALRLAAKLHCS
                     ETEMKTLEMAALLHDVGNVSAPDYILGKPGRLTEQEFRQAMRRAAVGAGIVAAAPFPY
                     AVEEIVRRRLDAFLEDLPAFVKDLERELKCERARSLLDQTPAEKVGQTWLSDAEANDS
                     VLRRITVEKLASAPERLISLYDMLHVLGSSLNTQQTIKQALVALRRVIPYDQAGVFLL
                     EGDHYSLAQGEGMPDYCISRFTLSAAHGALAHAAAARLPLLLPGAPVELAAGLNQYLA
                     NARESLVAPLVDHDAIVGGIMLCASSPDRLTQDHAWFLGLITPKLAATLRCARDVQAL
                     REEAATDPVTRLPNARAAFQRMEEEIRRASREGRRLAVLFLDLNHFKQVNDTYGHVVG
                     DKVLAETAEKLRQSLRPYDFLGRVGGDEFLAVLPGIDDENLRAKVASLKKLVEQNAVA
                     AGEGVAVSISVSVGVACFPRDGKDAEELMFRSDQRMYEDKRKIPAALSSPAPAPAAKT
                     LERAFVV"
     gene            5324..>6415
                     /locus_tag="VFA54_11065"
     CDS             5324..>6415
                     /locus_tag="VFA54_11065"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZS51394.1"
                     /translation="MAFCAPAAILLQAGALGDSGASYLAAVVGISVLSLLVAAFFSRY
                     VLGQDTGTAQMQKISNAIKEGAEAFLRRQNKTIIILALVLAVIIYLGYAFGKGDATLA
                     LRMTISFVIGSLCSLAAGFSGMWVSIRSNIRVASAARTDLNRALQIALRGGAVTGLSV
                     VALSLLGVGSLFWIFGGLDNPEQVPHQIIAFGFGASFVALFAQLGGGIYTKAADVGAD
                     LVGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGRGADLFESTAAENIGAMILGVSLYP
                     VFGLKGVLFPLMARAFGLIASILGVLIVRVKGDEDPMKSLNRGYLMTTVVALIGFFIA
                     VELLLQGNMMLFLAGVIGIVTSFLFVWITQ"
BASE COUNT         1098 a         2069 c         1932 g         1316 t
ORIGIN      
        1 cttcgtccag gcgctgctca gcggaaacgc cctcggcgtt cacgacgtgg aatcggcgct
       61 ccacggcact tcactgggcg tgcggctgag cgatggccgc ccggaagagc acggccatcg
      121 gaaccacatg cgcgccatca acgccattta tcattgcggc ggcatcgccc aggccgtaga
      181 gtgcggccgg ctcaattccg gcattctcta ccagtgcgtc aagagcggag tgccttttgt
      241 gctggccggc agcctgcgcg atgacggacc cttgcccgac acgatcaccg acatgaacgc
      301 cgcccaggac cgctatcagg agcaactgcg cggcgccggc cttgtgctgt gcctcgggtc
      361 gatgctccac tccatcgcca ccggcaatat gctgccttcg tgggtcaaaa tcgtctgcgt
      421 ggatatcaac ccggccgtcg ccaccaaggt ttccgaccgg ggcaccgggc aagccgtcgg
      481 cgtggtgacc gacgtcggcc tgttcctgga tttgctggcg aaacgtcttt ctcccggatg
      541 gcaaaagtga ctcgctcgca gaaaggttcg gcaaaaatga aaaatatttc ccgccgtgac
      601 ttgggccgct tggccgcagg agcgatgagc gccggcaagc tgggaagccc cggcttggga
      661 ctcgcggccg ccgccgcccc gcagcagccg aagtccgcct atgcgggtgc gcttaccgga
      721 gtcgagccgg ccaggcttga agaccgccgc ttcgatcctg tagcgtatac ccatgacttg
      781 tatgccgccg cgccccgccg cctgcggttt gaagcgcaga atctccggca ggcggaagcg
      841 tggcaaagaa aggcccgcgc caggcttatc gagctgatcg gcggtttccc ttccgggcgc
      901 gtcccgctgc ggccagtgac ccttgagaca cgggaattcc ctgcctacac tcgcgaaaaa
      961 gtcgtcttcg acagccgcaa aggcgtgagc gtgctggctt atgttctgat gcccaggcag
     1021 cccttgcgcc ggcccgtgcc cgccgtggtc tgcgtgccgg gccacggcag aggcgtggac
     1081 gacatcgtgg gcattgacga ccaggggcgc gatcgaacta acaaagacgg ctatcagcac
     1141 gacttcgcca tccaggtcgc cgaggccgga ctcgccgccg tggccatcga acaaatggcg
     1201 ttcggatgcc ggcgggatgc gatcacggcg aagaaaggtc ttggccagtc ggcttgccag
     1261 cccgcggccg gcggcgcgct gctggtcggc caaaccatga tcgcctggcg tgtttacgac
     1321 gtcatgcgca cgatcgattt catcgccgcc cggccggaac tcgattcctt gcgcgtcggc
     1381 tgcatgggaa tttccggcgg cggcacctgc accctgtttg cttcggcgct cgagccccgc
     1441 atccgcgcgg ccatggtcag cggctatctc aatacctttc gcgacagcat cggcagcctc
     1501 tcgcactgca tcgataatta cgttccgggc attttgaact ggttcgagat gtatgatgtc
     1561 gcgggactgg tcgcgccgcg ccctctgttc gccgaatcgg gcgaaaaaga cgagattttt
     1621 cccatccggg cgagcctgga gagctttcac agggtcaaag agatttattc ggtcttcggc
     1681 gtgcccggcc acgtggagca ggaaaccttc aacggccccc atagcttttg gggtaagaag
     1741 ggcattcctt ttctcgcgcg ccatctggcg gcctgatccc gtcgtgcacc cccggatttc
     1801 gcagcgttct tcacaaacct gaggctgtat atcccgcgcg ggccttgggg tgtaaactca
     1861 cccgtacggt ccgtaccgat ttgccatagc gtcaattcca ccgcctgact agaattgagc
     1921 ccagaaccgc tggagggcgc attggcagtc tgggatggct tttgggaaaa cctgtcccag
     1981 ctcttctctg ccattcgtga ttggttcaat cccaccccgc agatgggcgt cgctttcgcc
     2041 ctgtggctgc tgatcgccgg actccattat ggtttgatgc aggtccgccc ggccgcaggc
     2101 aaagcgaggc cgagccggga cctggtatgg atcgaccgcc tttcgagaat cgtttttccc
     2161 ttcgccaccg cgctgctggc ccttttcctg ctgcccgaat cgatcgtcga gcgcctggaa
     2221 ttcaaagctc agtcctatct gcatcaagcc gggatgctgg agctgcaccc gcttcgctgg
     2281 aagctggaaa ccggcgccgt cgccctgctg cgtttcgaag gcgagcggat tcatgtagac
     2341 gttttatccg gtcgcgaatc tcgcggcggt attcgcgtcg ctgagttgac caaacagggc
     2401 cgcggatact caggcgccgc gcaagtcggc atgccttgcc ctgcgggcgg cccgccgcac
     2461 gcctcctgca cgaaagaggt cccgatcgag ttcacgctcg tacaacagcg ccggattgaa
     2521 ggccgcctcg gcctcgatcc ggaaaaatcg caggccgctt gcccgcactg ccggacgcag
     2581 tggaaggaat ttgtctgggt tccggagtga gatcgctttt cgcgctgccg gctttcgcac
     2641 aactcggaag gggcaggttg aagccgcgcg gaaggaaaag ttcgccgggc aattcctccc
     2701 gccgtcgcgc agttcgctgc ctgcaatttg atcccgggaa accgaatcct gtctctagtg
     2761 gcggcttctt cggccgataa gactgtatga gtgatgcaaa gccgtttgct ccgggtctta
     2821 tacctgtgcc tgattctggc aggcgggctg ctcgcggcga tcgccatggc caggcagccg
     2881 ggcgagccaa acgcggcggc tttcgcgatc ctgctgttcc tggcatgtgg cacggctgcc
     2941 ctgaaggtgc agctgccggg cgtggatcgc tccgtttcgc tctccttcgc ctttgctttt
     3001 gcggccgtca ccgagttccc ggccgcccat gcctttttcg tcgccgccgt ttccttggtc
     3061 tatgccgcct tccaggagga aaagcgcgcc tggcaaccgc tcgatctcgc ctttaacctg
     3121 gcttcggctg cggtctctac cctctgtacc agcgaggttt atgcccatct ggggcgtgat
     3181 tttgaatccg ggcggctgat tgccatgggc acggccgccg tggtctatta cgtcttgacc
     3241 agcgggacca gcgctcttca aatcggtttc caaacgcgca cgcgcccgtg gaaggtgtgg
     3301 caggaaaagt tcttttggat gggacccctc taccttctgg tccctgtcgc caacggcatc
     3361 gctaagatgc tgcggcaaac cgcctgggcg cccaaccgtc tgctcggcct tatgctgatt
     3421 ttcggcggct atcgctacgt gaaacattac ttcggcaggc tgcatgacca gcaggatcac
     3481 gccaaacggc tcgatggcat ccgccagcgc accatcgaag ccctcgccgt ggctatcgag
     3541 gccaagaacg gcgccgccgc cggacatttg cagcgcgtcc gccggcacgc cctccggctc
     3601 gccgccaaac tgcattgctc cgagacggaa atgaaaaccc tggaaatggc cgctcttctg
     3661 cacgacgtag ggaacgtttc ggcgcccgat tatattctcg gcaagcccgg tcgcctgacg
     3721 gagcaggaat tccgccaggc gatgaggcgc gcggcggtgg gcgccggcat tgtcgccgcc
     3781 gctccgtttc cctacgcggt ggaagaaatc gtcaggcgcc gcctcgacgc cttcctggaa
     3841 gatctgcccg cctttgtaaa agatctcgag cgtgaactga aatgcgagcg ggcgcgcagt
     3901 ctcctcgatc aaactccggc ggagaaagtc gggcaaacct ggctctccga tgccgaagcc
     3961 aatgactccg tgctccggcg catcaccgtt gaaaagctgg cttcggcccc ggaacgccta
     4021 atctctcttt acgacatgct ccatgtcctc ggatccagct tgaacactca gcaaacgatc
     4081 aaacaggccc tcgtcgctct ccggcgcgtc attccttatg accaggccgg ggtctttctg
     4141 ctggaagggg atcattattc gctcgcgcag ggcgagggga tgcccgatta ctgcatttcg
     4201 cgcttcaccc tttccgccgc gcacggcgcc ttggcccatg cggccgccgc ccggctgccc
     4261 ctcttgctcc ctggcgctcc cgtcgagctg gccgcaggct tgaaccagta tctggcgaat
     4321 gcccgggaat ctctggtcgc cccgctcgtg gatcatgacg caattgtcgg cggcattatg
     4381 ctctgcgctt cctcccccga tcgcttgacg caagaccatg cctggtttct cggcctgatc
     4441 accccgaagc tcgccgccac tcttcgctgc gcgcgtgatg tccaggcgct gcgcgaagag
     4501 gccgccaccg accccgtcac ccgcctcccc aacgcccggg ccgctttcca gcgaatggaa
     4561 gaagaaatcc ggcgggccag ccgcgaaggc cgccggcttg ccgtgctgtt cctcgacttg
     4621 aatcacttca agcaggtcaa cgacacatat ggacatgtgg tcggcgacaa ggttctggcg
     4681 gagacggcgg aaaagctcag gcagtcgctc cggccgtacg acttcctcgg ccgggtgggc
     4741 ggcgacgagt tcctcgctgt cctgccggga atcgacgatg agaacctccg cgccaaggtg
     4801 gcatccttga aaaagctcgt cgagcaaaac gcggtcgctg ccggagaagg cgtagcagtt
     4861 tctatttcgg tcagcgtcgg cgtcgcctgc ttcccgcggg atggcaagga tgccgaggaa
     4921 ttgatgttcc gcagcgacca gaggatgtac gaggataaaa gaaaaatccc ggcggctctc
     4981 tccagccccg ctccggctcc agccgcaaaa acgctggagc gcgctttcgt cgtttaagct
     5041 acaattctga aatcggactg cactgggagt gccgggtagc cccgctgtca tcgctctcct
     5101 tcgcgaggct caaaggataa gaagcgatag aaattatcca ccccggcaca ttcgagtgag
     5161 catcagtccc tgcacgcgcc aagccaggct catagaatgt agatttcccg tccgcggcgg
     5221 ctgcgctgtg tttcgcccgg ctcggcctgg attgtgctat aagaaaggat tttggactca
     5281 ctaaaagaat tgtataacgg atatcccttg ggagggggat cgaatggctt tttgcgcgcc
     5341 ggcggccatc ttgctccaag ccggagctct tggcgactct ggggcctctt accttgcagc
     5401 cgtagtcggc atcagtgtgc tctcgctgct ggtggcggcg tttttctccc gttatgtgct
     5461 gggccaggat accggcacgg cgcagatgca gaagatctcg aacgccatta aagagggcgc
     5521 cgaggcgttt ctgcggcgcc agaacaaaac catcatcatt ctggcgctcg ttctcgccgt
     5581 gatcatctat ttgggctacg ccttcgggaa aggcgatgcc actctcgccc tgcggatgac
     5641 catttcgttc gtcatcggct ccctgtgctc gctggccgca ggcttcagcg ggatgtgggt
     5701 gtccatccgc agcaacattc gcgtggcgtc ggcggcgcgc accgatttga atcgcgcttt
     5761 gcagatcgcc ctccgcggcg gggccgtgac cggtctgagc gtcgtcgcgc tctcgctgct
     5821 cggcgtcggc agtttgttct ggatcttcgg cggcctcgac aacccggagc aggtgccgca
     5881 tcagatcatt gccttcgggt ttggcgccag cttcgtcgcc ttgttcgccc agcttggcgg
     5941 cggcatctac accaaagccg ccgatgtcgg cgccgacctc gtcggcaaag tcgaggccgg
     6001 catcccggaa gacgatcccc gcaacccggc cgtgattgcc gatctggttg gcgataacgt
     6061 cggcgactgc gccggccgcg gcgccgattt gttcgaatcc accgccgccg aaaacatcgg
     6121 cgccatgatt ctgggcgtct cgctctatcc cgttttcgga ctgaagggag tcctgttccc
     6181 gctaatggcc cgcgccttcg gcttgatcgc ctcgattctc ggcgtcctga tcgtccgggt
     6241 caagggtgat gaggacccga tgaagtcgct caaccgcggc tacctgatga ccaccgtggt
     6301 ggctctgatc ggattcttta tcgccgtcga gctccttctg caaggcaata tgatgctgtt
     6361 tctcgccggc gtcatcggca tcgtgaccag ctttttgttc gtctggatca cgcaa
//
LOCUS       DASREK010000213         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1921022, whole genome shotgun sequence.
ACCESSION   DASREK010000213 DASREK010000000
VERSION     DASREK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1921022"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1713)
                     /locus_tag="VFA54_11070"
     CDS             complement(370..1713)
                     /locus_tag="VFA54_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZS51395.1"
                     /translation="MKKQDGRRSHDLPSFARYLKKVFDFRTAAAGLTDSRVAPEIPTS
                     AVFLAAFHGFVFRLPSFQQLQAELRQSALQNWIGASRPFGDDVLRYSLSGFRLEGLET
                     MLVDVNRTLKRNKAFDSGRVQGRIVAAVDGIEVLSSYSRCCDSCLERRVVVHKGGVKH
                     EQLQYYHRAVACQIVGGPVKAFVAIEWLQPGEGEDTAALRMLGKIPELYGSRFFDILL
                     ADALYAQTPVLDLCSQIGWDAVISLKQNQRDLYQSAVRLFAQRPADATFDERRSGKSY
                     QVQLWDTEGLPFSGADPRLVRVLRSEETLAENHYRKREKQTEQTSHEWLWITTLDPKQ
                     FSAPQVRRLGHDRWILENNGWNDLTQHWALKHGFLHACKHRPRAPQPDGERQFVPNRG
                     LAAVTFILLLAFTLSSAFTHCHSKLVRRYGWSGLEVASQLRRSVSKLPPNIRAPD"
     gene            complement(1760..2143)
                     /locus_tag="VFA54_11075"
     CDS             complement(1760..2143)
                     /locus_tag="VFA54_11075"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HZS51396.1"
                     /translation="MKASGSLARLSELALRQRRQGLAQQLPPVTEVLRGSLVERYVTC
                     GNPACKCAKGERHGPQWYLTVTLGRGRTTGGVVPEENLPQVRAWIDNYHKLKEQLEKI
                     SDINRELLRRRREQERKRKAARRTE"
BASE COUNT          456 a          676 c          822 g          472 t
ORIGIN      
        1 ccagcggcgc gccgcccaaa atcgcggcgg ttgtactaag aaacgaacgg cgtttgatct
       61 ggcacatgga tcgccctcct cgggaatcgg tataaacatc agataccagt tttcacgcgg
      121 cgtcaagttc acccggtgga ccggcaggaa cttcgcatta ccgagccgac cacccggggc
      181 ggtgcctgat ttccgggcgc ttagccagag agggactggt cagggagatt cgcacacgcg
      241 ccaagaggct ggaatggagc ggaaatcgtg agtttttgga gggggagaga agcggcgagg
      301 gaaaggagga gcttgcggcc aagtacgccg ggcggcggtg caggtccaga tgtgcgggag
      361 cggtgggatt caatcgggag cgcggatatt gggcgggagt ttggagacgg agcgccggag
      421 ttgggaagcc acctcgaggc cgctccagcc atagcggcgg accagtttgg agtggcagtg
      481 ggtgaaggcc gacgataacg taaaagcaag cagcagaatg aaagtgactg cggccagtcc
      541 gcggttcggg acaaactgac gctcgccgtc gggctgggga gcacgggggc ggtgcttgca
      601 ggcatggaga aagccgtgct tcagggccca gtgttgagtg agatcgttcc agccgttgtt
      661 ttccaggatc cagcggtcgt gaccgaggcg gcggacttgg ggagcggaaa actgtttcgg
      721 atcgagggtg gtgatccaga gccactcgtg cgaggtttgt tcggtttgct tttcgcgttt
      781 gcggtaatga ttctcggcga gggtttcttc ggaacggagc acccgcacca ggcgcgggtc
      841 ggcgccggaa aaggggaggc cttcggtatc ccaaagctgc acttgatagc tcttgccgct
      901 gcgccgctcg tcgaacgtgg catcggccgg cctttgcgcg aatagccgta cggccgattg
      961 atacagatcg cgctggtttt gtttgaggct gatgaccgcg tcccaaccga tctggctgca
     1021 caggtccagc accggcgttt gagcgtagag cgcatccgcc aggaggatgt caaagaaacg
     1081 gctgccatag agttcgggga tcttgccgag catgcgcaag gcggcggtgt cctcgccttc
     1141 gccgggttgc agccactcga tggccacgaa cgctttcacc gggccgccga caatctgaca
     1201 ggcaacggcg cggtgataat actgcagctg ttcgtgcttg acgccgcctt tgtgaacgac
     1261 gacgcggcgc tccaggcagg agtcgcaaca ccggctgtaa ctggaaagca cctcgatgcc
     1321 atccacggcg gcgacgatgc ggccctgcac acggccggag tcgaaggcct tgttgcgctt
     1381 gagggtgcga ttgacgtcga ccagcatggt ctcgagtcct tccaggcgga aaccggacag
     1441 gctgtagcgc agcacgtcat cgccgaacgg gcgcgacgcc ccaatccagt tttgcagcgc
     1501 cgactgccgt agttcggctt ggagttgctg aaagctgggc aggcggaaga cgaagccatg
     1561 gaaggcggcc aagaaaacgg cggaggtcgg gatctcggga gccacgcgtg agtccgtcag
     1621 gccggccgca gccgtgcgga agtcgaaaac cttcttgaga tagcgggcga aagagggcag
     1681 gtcgtggctg cggcggccat cttgcttttt cataaatgca agatagggcg gagcccgatc
     1741 tttttcgcct cgccccgtat cactctgttc tccgcgcggc tttgcgtttg cgttcttgct
     1801 cgcgccggcg gcgcagcaac tcgcggttga tatccgagat cttctccagt tgttctttga
     1861 gtttgtggta attgtcgatc caggcgcgga cctgcggcag gttttcttcc ggcacgaccc
     1921 cgcccgtcgt gcgtccacgt cccaaggtga cggtcaaata ccactgcggc ccgtgccgct
     1981 cgcctttggc gcacttacag gcggggttgc cgcaggtgac gtagcgttcg accagagagc
     2041 cccgcagaac ttcagtgaca ggcggcagtt gctgggccaa gccttgacgg cgctgacgta
     2101 gagccagttc agagaggcga gctaggctgc cacttgcttt cacttaactg caagtttaag
     2161 cgaaaaccaa cgcttttcgc cagatgcgaa tctgctaatc tgcgaatttc ctcttatccg
     2221 cctgattctg aaggtaattt agttgccgtg cgaagttcct gggtcgcctc gaccgggcca
     2281 tgatgcttcg ccgggcatgc ggccccgccg gtcaggcccg cggcgcgact ccgggatcgg
     2341 cgagatcgag caaggtcacg ctttcctgtt cattcgggtc ggtgcgcgcg acgaggccca
     2401 tgcacggcgt ttcggcgctc ggatta
//
LOCUS       DASREK010000214        33784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1090767, whole genome shotgun sequence.
ACCESSION   DASREK010000214 DASREK010000000
VERSION     DASREK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 33784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33784
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090767"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..3955
                     /gene="rpoB"
                     /locus_tag="VFA54_11080"
     CDS             2..3955
                     /gene="rpoB"
                     /locus_tag="VFA54_11080"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZS51397.1"
                     /translation="MPCGRCGTYNKNIVTFCNKCGDPVGLQLKYDMAECQERGMTFAA
                     PLKVTIRLTVYEKNPETNEKSIRDIKEQEVFFGEIPLMTENGTFMINGTERVIVSQLH
                     RSPGVFFERQVATNYFLGKIIPYRGSWVEFEYDNKNLLYVRIDRKRKFLGTIFLRALG
                     LKNDESIIKTFYTVDKITIKNKKLYWAVSEGLVGLKLTHNVVSKGGDIVVPSGRKITP
                     SVLKEIQKAKIQQIEVSANDLEGAFTAADVVDLNSGEVLADANTELTTSLVSALLDSG
                     VDQFEVFFPERDQTGVVISQTLKKDTLKTQKDALVEIYRKLRPGDPPTLETATQLFRS
                     MFFDPRKYDFSRVGRMKFNIKLFDKIDATPLDKRTLDPEDFYAVILYLLKLRRNIGVV
                     DDIDHLGNRRVRAVGELLENQFRIGLVRMERAIKEKMSVYQEMSTAMPHDLVNAKPVM
                     AAIREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPTHYGR
                     ICPIETPEGPNIGLISSLSCFARINEYGFIESPYRKVEKGYVIDEVKIVNPGDTDFKA
                     GDVVRRQDAEKANRELSGRKKPAEWTPYCHYLSAWEEDKYVVAQANVEIDEKGRIVPE
                     LVNARQSGNFALKHREEVEYIDVSPKQLVSVAASLIPFLENDDANRALMGSNMQRQAV
                     PLLRAEAPYVGTGMERVTAHDSGAVVLCKRSGVVDSVDSERIIVRVEGATQEGQLSRE
                     VGADIYTLTKFKRSNQNTCINQKPIVRQGERVVKGQVLADGPCTDRGELALGRNVLAA
                     FMPWRGYNFEDAILVSEKLVKEDYYTSIHIEEFEIEARDTKLGPEEITRDIPNISETF
                     LANLDESGIIRIGATVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVRDASLY
                     CPPGIEGTIVDIKIFSRKGAEKDERAKAIEEEQVSRLQRNLQDEVRILNDERAKRLMA
                     LLEGKQVLADLHDERTNKRLLSKGAEITRDIVEKLKARDLKRVRFKDKDPRLNEQIDE
                     IEEMTVRQIQVLEKITDEKIAKLKKGDELPPGVIKMVKVYIAMKRKLSVGDKMAGRHG
                     NKGVIARILPEEDMPYLPDGTPVEIVLNPLGVPSRMNVGQILETHLGWAAKALGVHFA
                     TPVFDGATEREIKQQLSKAGLPTSGKTVLYDGLTGNRFEQEVTVGYIYMLKLSHLVDD
                     KIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQELLTAKSDDVY
                     GRAKIYEAIVKGEAAMEPGVPESFNVLIRELQSLCLDVELIKKQREMVDTALAAD"
     gene            4033..8241
                     /gene="rpoC"
                     /locus_tag="VFA54_11085"
     CDS             4033..8241
                     /gene="rpoC"
                     /locus_tag="VFA54_11085"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZS51398.1"
                     /translation="MYRSSPYDRANLIADFDSIRISLASPEKIRSWSHGEVTKPETIN
                     YRTFKPERDGLFCARIFGPVTDWECLCGKFKRMKHRGVICDKCGVEVTLSRVRRERLG
                     HIELASPCSHVWFFKGLPSRIGHLLDITLRELERVLYFEAYVVVDPGEVEGLSPGEVI
                     TDEKKRQLEQEHSGKFVAMMGAEGIKELLKRVNVDKLSEELREKMKTETSLQKKLKYA
                     KRLKVVESFRKSGNKPEWMILDVLPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKKLMELHAPDVIVRNEKRMLQEAVDALFDNGRRGRVLRGANNRPLKSLSDTLKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPELKLNQCGLPKKMALELFKPFIYQRLEQRGHCT
                     TIKQAKEMVEQQEPMVWDILEEVIKDHPVLLNRAPTLHRLGIQAFEPVLVEGKAIKIH
                     PLVCTAFNADFDGDQMAVHVPLSPEAQIEAAVLMLSSNNILSPAHGAPIAIPSQDMVL
                     GCYYLTKARPGARGEGRTFASTEDVLIALEMGEVETLTPIKLRFTGKVIDLVHAFDDQ
                     NIIHAEPIEFNKQYMETTVGRVIFNDHLPSGMPFINGLLKKKGLGQLVQYCYLRFGLQ
                     ATVKMLDELKTLGFLYATHAGISIGIDDMVVPAEKQQLIDQAQKEVIDVEQQYLDGAI
                     THGERYNKIIEIWSKVTEKVSDEMFSAMEGQDKSGEIINPIYIMADSGARGSKQQIRQ
                     LSGMRGLMAKPSGEIIETPITANFREGLNVLQYFISTHGARKGLADTALKTADSGYLT
                     RRLVDVAQDVIVSERDCGTTEGIFVEPIIESGEIIEPLRDRIVGRVSLEEIRDYEGNL
                     IVGINQEIDEELAAAIQRAGIERVKIRSVLTCESKRGVCVMCYGRNLASGRLVERGEA
                     VGVIAAQSIGEPGTQLTMRTFHIGGTATRVSEQSTQDSKSNGFAKFLNIVTVRNKNND
                     LVAMNRNGILAIVDEKGREKERYPVVYGAKVRVEDGQPVSINQILLEWDPYTFSILTE
                     VGGVVHFRDLQEGVTVQEQVDEITGMSQWVVTDSPDEKRQPTIEIRSENGKVLRKYLM
                     PTHSHLMVRDGEAVHAGDVLAKIPRETTKTKDITGGLPRVVELFEARKPRETAVIAEI
                     DGVVKYGELVKGQRKIEVVPDSGETRVYTLPRGVHINVQEGERVQAGEPLMDGPRNPH
                     DILAVLGEKELQKYLVNEIQEVYRLQGVNINDKHLEVIVRQMMRWIKVEEVGDTDFLL
                     EEVVDKFKFREENERVMAAGGTPAVGRPLLMGITKASLSTDSFISAASFQETTRVLTE
                     AAISGKIDYLRGLKENVIMGRLIPAGTGMLHYRNVQVPGEPVPEPEIEETDVLAGIPG
                     YAEEARVEYAGGLSEDNSEGLPVE"
     gene            8373..8543
                     /locus_tag="VFA54_11090"
     CDS             8373..8543
                     /locus_tag="VFA54_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZS51399.1"
                     /translation="MPNLGMPELLVILGVAVLLFGGRKIPEIGKGLGEGIRQFKNALK
                     GEEKVEEKKPQA"
     gene            complement(8670..8957)
                     /locus_tag="VFA54_11095"
     CDS             complement(8670..8957)
                     /locus_tag="VFA54_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZS51400.1"
                     /translation="MTQTQLVRTLAEACEVNNRSARKFLDELANVAIRETKKSGVFVL
                     PGIGRLVRVDRKARMGRNPATGEAIKIPAKKVVKFRVAKAAKDAIVPPKKK"
     gene            9154..10500
                     /gene="trmFO"
                     /locus_tag="VFA54_11100"
     CDS             9154..10500
                     /gene="trmFO"
                     /locus_tag="VFA54_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HZS51401.1"
                     /translation="MAVIRVIGGGLAGSEAALQIARAGHRALLFEMRPLASTPVHRTG
                     CFGELVCSNSLKSEQPGTAPWLLKQELRRLGSALMEAAGRCRIPGGHALTVDREAFAA
                     EVTRRVEEEPNIGIRREEAAEIPGGDITIVASGPLTSQRLATAISRFTGQDRLYFFDS
                     ISPIVEADSIDYSVVFRASRYGKSLDGTEDYLNCPFTREQYDRFYDALVSAEPAAVHG
                     EEDTPFFEACLPIEELARRGRDTLRFGPMKPVGLTDPRTGRTPYAVVQLRQENLRAES
                     YNLVGFQNHLKYGEQKRVLRLIPGLENAEFTRFGQIHRNTYINAPALLSPTLNLRSAP
                     WIFFAGQISGVEGYVEAIATGLMAARHAISLAEGKTPVPPPRETALGSLAHYISHADP
                     RSYQPANITFDLLPALPEADRCNLGRDKKARRELQCARAAEALEKYCAEYEALCVR"
     gene            10478..11371
                     /gene="xerA"
                     /locus_tag="VFA54_11105"
     CDS             10478..11371
                     /gene="xerA"
                     /locus_tag="VFA54_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HZS51402.1"
                     /translation="MKHSASGELSSAIERFLRSLAVANVSPHTLRNYASDLKQFAEYL
                     SPPGSAAPALKAIDHLHIREFLGALYQRKNSRRTVARKLAALRAFFRFLTREGSLAAN
                     PARLATTPKTPKALPPVLSAEQANSLVDAAGREGSPRDRVIFELLYGCGLRVSELAGL
                     NLDDFDWSQRWIRVRGKGRKERLVPFGTKAAEALERYMQGRAGCGGQALLLNPRGGRL
                     TSRSIERIVKRYAILFSADPSLHPHSLRHAFATHLLSDGADLRAIQELLGHASLSTTQ
                     KYTQLSLKELMEVYDRAHPRA"
     gene            complement(11387..12538)
                     /gene="wecB"
                     /locus_tag="VFA54_11110"
     CDS             complement(11387..12538)
                     /gene="wecB"
                     /locus_tag="VFA54_11110"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZS51403.1"
                     /translation="MPLSAALSRPARVLVVFGTRPEAIKLCPLIRRLRERPESFQVRI
                     CVTAQHRSMLDQVLQAFQVQPDYDLDVMRPDQTLPQLTSRAMAALEPVLNLEKPDTVI
                     VQGDTTTTFCAALAGFYAGIRIAHVEAGLRTGDKYQPYPEEINRLLTSRLSDLHFAAT
                     RQAAANLRAEGIPAASIFVTGNTVIDAVLEIRRGLESGRLLRPDWPFLARGRRLILVT
                     AHRRESFGEGLERICEALVKLSERPDIDVVYPVHCNPRVQGPVHSRLSSRPRIHLIEP
                     LEYVPFVDLMARSYLILTDSGGVQEEAPSLGKPVLVMREKTERPEAVRAGTVRLVGTN
                     VETIVTETNALLDDAGRYRRMARRHNPYGDGHACERIAEILAGKALSPR"
     gene            complement(12542..13372)
                     /locus_tag="VFA54_11115"
     CDS             complement(12542..13372)
                     /locus_tag="VFA54_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HZS51404.1"
                     /translation="MRDERTLLQVLDQPDDQIDLGRAALLLARCECPGLDVERELARL
                     EALAEAARLPLSIHKASLDRVKALRLFLAETCGFRGNEQDYYDPKNSCLNHVLDRRVG
                     IPITLSVLYMEVGRRLGAPLAGVGLPGHFLLKYYDSNDDALFIDPFHGGRFVDSSGCR
                     DLVRRMYEGRLSFHPSFLQPVDKRYIVLRMLNNLRSIYVHGQQFSKALRVIEMILAIT
                     PASSDDLKQRGLIHYKLGHYKQARQDLEACLFLNPQSADAEAIQQTIAELKRLTAMLN
                     "
     gene            13577..13822
                     /locus_tag="VFA54_11120"
     CDS             13577..13822
                     /locus_tag="VFA54_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51405.1"
                     /translation="MIFLSLLMNLMLMFSTGVGLNMATPNAVLDPQISGEHRTHGHLP
                     GPPDGHNGDSHLHHGQHGHSVGPSGGGDHLPPGHTNT"
     gene            complement(13993..15288)
                     /locus_tag="VFA54_11125"
     CDS             complement(13993..15288)
                     /locus_tag="VFA54_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS51406.1"
                     /translation="MRNRVWPWLVLGAVTLGLTRGSAQAPPRKLTLRQAEELAAKNHP
                     QVSAAFLTALAANQVTIETRSAFFPTVFGSLTGAGAAKDSRITAGLLNNPSIFNRFAG
                     GFAINQLVTDFGRTSNLAENARLRAQAEEQTAQATRAQILLQADRAYFAVLRAQAVLT
                     VAQQTVSARQLVADQVTALAQSKLKSDLDVSFARVNLSEARLLLIDAQNEIRAAFAQL
                     TAALGFQDEQTYELAEEPMPGAPPASLADLVAQGLRDRPELASSRLSRDAAQKFAAAE
                     RDLARPTISAVGVAGGSPAHDSFITDHYSAAGVNVNIPIFNGHLFTARRTEADLRAQA
                     QEQQVRDLENAIARDIRVAWLNANTAYQRLSVTDELLQQATLAQDLAQARYDLGLGSI
                     VELSQAQLNKTSAQIASAEAKYDYQIQRSVLSYQIGALH"
     gene            complement(15285..18461)
                     /locus_tag="VFA54_11130"
     CDS             complement(15285..18461)
                     /locus_tag="VFA54_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS51407.1"
                     /translation="MSRFAIKTPYFIVVVCLVIAVVGVASITRMPVDMFPAMNIPVVV
                     VATFYSGMPPEQIETDITSRFERFFTLGSGIDHIESRSLTGVSVIKVYFQPGTNPDSA
                     VTTISNLAMAQLRRLPPGTLPPVVLKFDASSLPVCLVTFEGQGLNETSLRDLAQFNVR
                     NQIASVPGASVPQPFGGLYRQIMVYADPYKLEAHQLSLMDVVRAVNDANLILPAGDVQ
                     LGPIDYNIYTNSQLSTVKDINDLPLKTIGQNPVRVSDIGEAKDAQQIQTNLVRVNGQR
                     SVYLPILKQGGDTNTIQVVDGVKQTVAHLFDVPKELVTKVVFDQSEFVKTAIETLLHE
                     GAIGLLLTSLMILIFLGSMRATIAVFFSIPLSALATFIALWLGNGSINSMVLGGLALA
                     FSRLIDNSVVVLENIYRHLEMGEPPRVAAENGGREVALPVLAATLTTVVVFFPVTFLY
                     GVSKFLFSALGLAVVLSLFASFAVAMTVVPLFCAFFIKRPHGHHAEEAAGEEETPAPR
                     SFGERFNLWFNRHFENTLKGYDKAVRATLKRPAFVLAAFAAVFAVSFTLWPRLGLAFF
                     PRTDAGQFVINLKAPSGTRLAVTESEIAKVEALIRKIVSPEDLGLIVSNIGVTPDFSA
                     IYTPNSAMHTAFVQVSLKDGHKVGSYEYMERVEKAMAAELPELTTYFQSGGLVDAVLN
                     LGLPAPIDVQVAGADLERSYQTALSLASRIKNIPNVAGVYIPQDLDYPALHLDIDRTR
                     AAELGLSQREIVDNVITALTSNGMIAPSYWIDPKTGNDYMLTVQYPELQIGSLLDLRA
                     IPLHGRISANPTRLDMVSKITRVNSPTEVDHYQLRRTIDVFVRPLSEDLGKIADAIDA
                     TISHMKIPDGTSVTLRGMVQGMRASFRSFGLGLTLAVVLLYLILVAQFASFIDPFIIL
                     LAVPPSITGVIWTLYLTDTTLNVMSLMGVVMLVGIAVSNSILIVEFTRRLRADGMTVP
                     AAVALACRVRLRPVLMTSLATIIGLLPMAMKLGAGSEAYAPLARAIIGGLAISVVFTV
                     ILVPAGYYLVYRRQDAAPGAEHPQGAAQ"
     gene            complement(18464..19702)
                     /locus_tag="VFA54_11135"
     CDS             complement(18464..19702)
                     /locus_tag="VFA54_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS51408.1"
                     /translation="MLTRVYTGVAAGLAIVFAVIVSSCSRPAKTEAGTADPPKALIVA
                     VAKCERADLARDLVLTAEFRPYQEVDVMAKVAGYVKQINVDIGDRVREGQLLATLEVP
                     EMADDLARAAAAVKRNTAELERAKQELRRANSAHEIAHLTADRLAGVMKTRPGLVAQQ
                     EVDDARSRDLVAEAQVEAAKSALAAAEEQVRVAEAEQARTKTIFEYTQVTAPFTGVVT
                     KRYADTGSMIQAGTASQTQAMPVVRLSQNSLLRLILPVPESAVPTVHVGQPVEVKVPS
                     LNRTFPGKVVRFADKLNLSTRTMDTEVDVPNPDLVLIPGMYATVNLTLQQRNDALAIP
                     LTAIGGSDQKPTVFLVRPDGTVEERAVTLGLETANRVEVRAGLSEGDLVVIGSRNQIH
                     PGQQVSPKLVEMAAAKEEGQ"
     gene            complement(19714..20406)
                     /locus_tag="VFA54_11140"
     CDS             complement(19714..20406)
                     /locus_tag="VFA54_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZS51409.1"
                     /translation="MESILSFDQLEAIRRLDACTLSNAIETFDLRLRNEGFADASIRC
                     MFPQLPPMLGYAVTARIRGSGPPPVGHSYFDRGDWWSYILTVPGPRVTVVQDLDDPPG
                     VGGFVGEVHASIHRALGCVGTVTNGGVRDLPAVEKMGYQLFAAHPSVSHAYVHMVEFG
                     EPVEIGGLTIRSGDLIFGDCHGILSIPREIAADLPAVAARMLAREQRVIGLCRSPTFS
                     LEKLREIIKELG"
     gene            complement(20477..20794)
                     /locus_tag="VFA54_11145"
     CDS             complement(20477..20794)
                     /locus_tag="VFA54_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51410.1"
                     /translation="MPRAGLAFRFSRAGILLLAGLLFALSTQAKLSLYHQDGPTKYLS
                     NAAKMKECRAQGVEAPFAEKTVEIRADDSAREFCVAPREVVLPRFFSLVRPFEFRPPP
                     AAC"
     gene            complement(20869..21459)
                     /locus_tag="VFA54_11150"
     CDS             complement(20869..21459)
                     /locus_tag="VFA54_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HZS51411.1"
                     /translation="MLLSRAFISYLARHLTKRLGDGPLEIADPEAVREAVQNLIIDEL
                     SVEDQINDEVREILGQYSDYMRREGVSYQEMFKRTKRLLLSERKIIRASGRETGDPTK
                     LSRDKINELSHKLAALLRRLPRVKFKMEWNDVRLEIVRGLTELLVVEDKIDHAARVKI
                     KSQKREIPEGSEEWELLFRRYYDEEMKKYGIDLANV"
     gene            complement(21598..22920)
                     /locus_tag="VFA54_11155"
     CDS             complement(21598..22920)
                     /locus_tag="VFA54_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS51412.1"
                     /translation="MNKTAKRLAATLPVSLLAAATVWAQAPADGETRALTLQQAVALA
                     LRQNPDYLLARLEEQRSRQAIAEARAPFIPQIAVGSGLAYSNGFPLSISGSAPSIFQA
                     YGSKFIYNRPQSMRVREAAEMATAASRSREGKAEEIAYRVAANYLDFERATRAAAAAR
                     SQLDLVTRIEGLVRARVEEGRLIPLDLTRARVETARARAQVENLQARAEFLEDMLRAD
                     LGLDENVRIAPVESGLPAETVLPETEESAVADALANSPELKRLQATLRAKEFDVKAEK
                     GAYFPRIDLVAQYAMLGRYNNYEEFFKTFQRNNGQLGMSFQVPLLARSQISARLAAVQ
                     SEISQVKLQISAAQSNLAVETRRLFREVRQAETARDLARMELDLARETLSVYLARLDE
                     GRISMNEVEQARVAESQKWEAFYDAQTAAAKARLNLLRETGTLVAALR"
     gene            complement(22913..24115)
                     /locus_tag="VFA54_11160"
     CDS             complement(22913..24115)
                     /locus_tag="VFA54_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS51413.1"
                     /translation="MLLDTIRFAFTALWAHKLRTFLTGLGMVIGNASVILVATVALAG
                     RAFVVRLIEGVGSNLIYAYYEPGENVAGGMQSDYITPDDVAAVREELGDAASGVAGVI
                     KIYDRMLLDSRQRDVAVLGSDTDYKIVRHLTVVAGRFYDDAEIRDRDKVCLLTTDLAT
                     RLFGSPQNAIGGSIKVHGLQFTIIGAFREGIPTFGQSEISGESVLIPMSVMEYFTERP
                     RVDPLYVSVRSPDEVEATTRLVRQILTSRHRPGSLYTVNNLAALLSTARRISFALTIV
                     LFLVSAIALTISGIFIMNIMLVTVTERTREIGIRMALGATRRQVLIQFLTEAGAISFV
                     GGVAGILVGVSAPLVARHFLEGVEIPISAVAIVAALLVSCGVGVVFGLLPASRAARLS
                     PTDALRYE"
     gene            complement(24459..25379)
                     /locus_tag="VFA54_11165"
     CDS             complement(24459..25379)
                     /locus_tag="VFA54_11165"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS51414.1"
                     /translation="MNVLLLGLLAFSSWTALQAQNPNTGTPPREAEEAKEAPAKTREA
                     EPDPPALDPRIKQPDVKEGAKNPVENAAAESLFQRGVQAYGAENYEAAIDFLEKAVQA
                     APGVSRYHDWLGKAYGRRAERVIFIRAIVLARKARNEFARAAELDPTNIEAWSDLLDY
                     DLQAPGFLGGGLDRAKAVAAKLATLNAAEGHRAQALIRAAEKDHAGAEREYRAALALE
                     PANPGRLLELASFLNERHRYAEADALFDQAEKLAPDSPDYLFTRGQQLVASKRDPIRA
                     RQLLQAYLRSNRKPEDPPPSEVQGLLKKLL"
     gene            complement(25376..26590)
                     /locus_tag="VFA54_11170"
     CDS             complement(25376..26590)
                     /locus_tag="VFA54_11170"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS51415.1"
                     /translation="MKRAALTLVLVALGVALWLHFRQPEPPEAPAARVKRGDLVQTLT
                     TNGKVEAFDSVDVSARAGLSVIKVNVQEGDQVRRGQLLAEVDTAAAREALAHAQAQLE
                     AARADEAVLERGGSSAEIAQIDSAIAEARLERETAEKEIAVLQRLVEKGAAPRVELQE
                     QQRRLIKAEMDMAALERKRKSFLGPEDRERIQARVREAQAAVAQAMTALSQTEVRAPI
                     SGTLYMLALRPGGYYAPGAVVAKVGLLDKVRVRLLVDEPELGPVRIGQPVRITWDALP
                     GVEWEGSVERLPSLIQTVGTRNVGELLCTIDTRERRLLPNVTVNVAIRTGEARNALTI
                     PREALVREGDKTMALVVDANGMVSRQAVRLGIHDLGRVQVLEGLADNQIVLLPGEHIY
                     SPGQQVRPKITT"
     gene            complement(26587..27393)
                     /locus_tag="VFA54_11175"
     CDS             complement(26587..27393)
                     /locus_tag="VFA54_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51416.1"
                     /translation="MRIVGTFILGILSAIAALAAGNFRLYLKDGSDHLVREYQVLSDR
                     VRFYSVERSQWEELPLVLVDLDRTERERKRIEEEQQQRAEEDRAEREAERRFRTELQR
                     VPLDDGVYWVNGDQTTPLKQGDPKVKGNKRRSVLKVIAPIPVVAGKATVELEGPHSAF
                     VVNSPKPEFYVRLDRVERLGIARLTVKKDSRIVEDLTIIPVSKEVMEQQKEVEVFRQQ
                     LAPTVYKIWPVDPIEPGEYAVVQFASSGQDLQDSARVNIQVWDFAYRKPN"
     gene            27514..28227
                     /locus_tag="VFA54_11180"
     CDS             27514..28227
                     /locus_tag="VFA54_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51417.1"
                     /translation="MTPATHVVAGAAAAARFRNAGAALAAAFLLHFFLDAVYHFEAFY
                     ELSVPAQWSYSYTMFMLFGGLLLLGAPVFVHLARHDPAVSSFGCYALLMCALAFEPKW
                     DWKLFWGAMLSAFWLLITRAPEPRKWILCGFVSYLPDCLKKPIPLLARLHDAAHYRSD
                     LDLGDWVSLLGRGRWRIPVNDRIFDPYYQAGYVLEILLEAVILFGCLYWLSRGRAAAT
                     DHPAGRQSDAIAEGSLIRK"
     gene            complement(28326..29732)
                     /locus_tag="VFA54_11185"
     CDS             complement(28326..29732)
                     /locus_tag="VFA54_11185"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZS51418.1"
                     /translation="MISRLPLSLFLLFSAALMQAQKIDRTKPPDTPPLPVYKLPPVFE
                     TRLPNGLLLVLVQDNRFPLVTVRLDFQAGSKFDPADLPGLSENVGALLKEGTTTRSSR
                     QIAEELASIGGSLNVSSSRDTLIIGANALSENTSRLLDLVADVARNATFPSDEVELRK
                     QNRKQSLLLQRSRADFQGELKFNEIVYGPHPYSRISPTPESIDKIDAAKLTAFRDQFL
                     VPNNAVLIVLGQLPQRDKVMDLVRQKFGEWRQKPLPAPPKAEFPESRRTLVLVDRPGS
                     VQADIRVGRLAVTRTSPEYFPMVLANAILGTGTSSRLFNDIREKRGYAYDAHSVLDAQ
                     KDAGVFEAVTQVRNEVIEPAVEAMLSNLEVMSKTPVGDSELRDFKNFLSGFFVMRLES
                     QNGLATQLALVKSMGLPNDYLETYTTRIRSVEPDQILSAAKKYISPEQAAIVVVGDAQ
                     KIAPALEKIGKFEVTKVQ"
     gene            complement(29729..31111)
                     /locus_tag="VFA54_11190"
     CDS             complement(29729..31111)
                     /locus_tag="VFA54_11190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZS51419.1"
                     /translation="MKLLLLCAIAAMAALAQTAEFRIPIEKFKLRNGMRVVLSRDNAV
                     PVVAVYLMYGVGARSEQKGRSGFAHLFEHMMFEGSANAPKGMHFKLVESNGGILNGST
                     HSDFTDYFEVLPSNKLGVALWLESDRMRSLAITAENLANQKEAVKQERRLSFDNQPYN
                     TAIIERWPELAFRNWQSAHSIIGSFEDLNAASVEDVAQFFKIHYAPNNAVLAIVGDIQ
                     IAEAKKLVETYFGDIPAQPQPAPPDLREPSDSVARSDVYHDPLAQVPAVIIGYPGPPR
                     RSPDYNALVMADVLLTGGESSRFQLNLVKGKQSVVRYEANLGWPVGSASDYKDPGLYG
                     IFILRKPNFTGKQIVDQVQEEIGKIQKDGIDPKEMQRARTFLRASRLNELQSSLNRAR
                     LLAQYEMFDGKPEWITEEINAFVAVTPAQVQAAVKKYLAPEKRTVLEILPAPKPASPK
                     PQAAPPKEAP"
     gene            31377..33506
                     /locus_tag="VFA54_11195"
     CDS             31377..33506
                     /locus_tag="VFA54_11195"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HZS51420.1"
                     /translation="MSRSGASLADGFFQYSLLLLVATGFAAVLSTGRLDEPTAALAAA
                     ALLGRAFIIKGWMKPPLSAFWVQALTIAYILFYPVDFLYLSGSFLGATVRMIFFLASI
                     KLLTANHGRDYLYLSIIALLEVLAAAMLTSSASILGFLALFLVFAVAARTRYEIQRNA
                     EVAVHKPAAGGSLGWRLSVLSLALAGGIALLALALFFVVPRAAGAYLSRLPAAGESVL
                     GFSDEIVLGTPGRVRRDKTPVMRVKILEGPPLNNLKWRGDALQYFDGVKWSNPPSIGH
                     VLAGAQGRYIVGRLRRRSRLLPGAILRYRVLRAPMDCDSLFLPGTPQILEGDFGRLEV
                     SDTDSISTPGGRWKPLRYEGWSFIGPARPAEAGDVEGPFQTRIRSRYLQLPALDPRIP
                     ALARQITEAEMSPYSRAVAIERYLRTHFGYTLELPLERLSDPLADFLFNRKKGHCEYF
                     ASAMAVMLRSIGIPARVADGFQSGVYNPVSGYYTIRASEAHAWVEAYFAGLGWATFDP
                     TPPEPAPEALLGRSWQYLDALETFWNDWIIQYDVNRQASLARSVQIEWYKAGYETMER
                     WDRVWLWAGGRWERWKQKPAQAVAGTLQVLAGFLAACVAVWALPRVRAWVVTQRVSLG
                     RGSHKDCALLYERALAQMQRRGFNRETWQTAGEFAKTVQPPECPAAVCALLGQITQSY
                     ERARFGCDGEAGRRLALLIRTLERSPV"
BASE COUNT         6645 a        10050 c        10492 g         6597 t
ORIGIN      
        1 aattccctgc ggccggtgcg gcacttataa caagaacatc gtcacgttct gcaataagtg
       61 cggcgatccg gtgggcctcc agttgaagta cgacatggcc gagtgccagg agcgcggcat
      121 gaccttcgct gcgccgttga aggtgactat ccggctgacg gtctacgaga agaacccgga
      181 gaccaacgag aaatcgatcc gcgacatcaa ggagcaggaa gttttcttcg gcgagattcc
      241 gttgatgacc gaaaacggaa cgttcatgat caacgggacc gaacgcgtga tcgtttccca
      301 gcttcaccgc tcgcccggcg tgttctttga gcggcaggtg gcgacaaact atttcctggg
      361 caagattatt ccctatcgcg ggagctgggt cgagtttgaa tacgacaaca agaacctgct
      421 atacgtccgc atcgaccgca agcgcaagtt tttgggcact atctttttgc gcgcgttggg
      481 gctgaagaac gacgaatcga tcatcaaaac cttttacacg gtcgataaga tcacgatcaa
      541 gaacaagaag ctgtactggg cggtttctga agggctggtc gggctgaagc taacccacaa
      601 tgtggtttca aaaggcggcg atatcgtggt tccctccggg cgcaagatta cgccgtcggt
      661 gttgaaggaa attcagaaag ccaagattca acagatcgag gtttcggcca acgatctgga
      721 gggcgccttt acggcggcgg acgtagtgga cctgaactcg ggcgaggtgc tcgccgacgc
      781 caataccgag ctgacgacgt ctctggtttc agccctgctc gactccgggg tcgaccagtt
      841 tgaggttttc ttccccgagc gggatcaaac gggcgtggtt atctcgcaaa ccctcaagaa
      901 ggacactctg aaaacgcaaa aggacgccct ggtggagatt taccgcaaat tgcggccggg
      961 cgacccgccc accctggaaa ccgccacgca gcttttccgc agcatgtttt tcgacccgcg
     1021 caagtatgat ttctcccgcg tgggccggat gaagttcaat atcaagctgt tcgacaagat
     1081 cgatgccacg ccgctcgata agcggactct cgacccggag gatttctacg ccgtcattct
     1141 gtatctgctg aagctgcgcc gcaacatcgg cgtggtggac gacatcgacc acctgggcaa
     1201 ccggcgcgtg cgggcggtag gcgagctgct ggaaaaccag ttccgtatcg gcctggtccg
     1261 gatggagcga gcgatcaagg aaaagatgtc ggtttaccag gaaatgtcta cggccatgcc
     1321 gcacgacttg gtcaacgcga agccggtgat ggcggccatc cgggagttct tcggctccag
     1381 ccagctttcc cagttcatgg accagaccaa cccgctcagc gagatcacac acaagcggcg
     1441 gctttcggcg ctgggacccg gaggactgtc gcgcgagcgg gcgggctttg aggtgcgcga
     1501 cgttcatcca acccattatg gccgcatctg ccccatcgaa acgccggaag gtcccaatat
     1561 cggcttaatc agttcgctct cctgctttgc gcggatcaat gagtacggct tcatcgaaag
     1621 cccgtaccgc aaagtcgaaa aaggctacgt gattgatgag gtgaagatcg taaacccggg
     1681 tgatacggac ttcaaagccg gcgacgtggt gcgaaggcag gacgcggaaa aggccaaccg
     1741 cgagctgtcg ggccgcaaga agcccgcgga atggacgcca tattgccact acctctcggc
     1801 ctgggaagag gacaaatacg tggtggctca ggccaacgtg gagatcgacg agaaggggcg
     1861 catcgtgccg gagctggtga acgcccgcca atcggggaac ttcgcgctga agcaccgtga
     1921 agaagtcgag tatatcgacg tcagccccaa acagcttgtt tccgtggccg ccagcctgat
     1981 tccgtttctg gagaacgacg acgcgaaccg cgctctgatg ggatcgaaca tgcagcgcca
     2041 ggcggttccg ctgctgcggg ccgaggctcc ttacgtgggc accgggatgg agcgcgttac
     2101 agcgcacgat tcgggagccg tggtgctgtg caagcgcagc ggtgtggtcg attcagtgga
     2161 ttccgagcgc attatcgtcc gcgtggaggg ggccacacag gaagggcagc tttcccgcga
     2221 ggtgggcgcg gatatttata cgctcaccaa attcaagcgg tccaatcaga atacctgcat
     2281 caaccagaag ccgatcgtaa ggcagggcga gcgggtggtg aaagggcagg tgctggcgga
     2341 cggtccttgc acggatcgcg gcgagctcgc gctcggccgc aatgtgctgg cggcctttat
     2401 gccctggcgg gggtacaact ttgaagacgc catcctggtg agcgaaaagc ttgtcaaaga
     2461 ggattactac acctcgatcc acattgagga gttcgagatc gaggcccgcg ataccaagct
     2521 ggggcccgag gaaatcacgc gcgatattcc caacatttcc gagacgtttc tggccaacct
     2581 cgatgaaagc ggcattatcc gcattggcgc cacggtgaag cccggggaca ttttggtggg
     2641 caaggtcact cccaagggcg agacgcaact gacccccgag gagaaactgc tgcgggcgat
     2701 cttcggagaa aaagccggcg acgtgcgcga tgcatcgctg tattgccccc ccggcatcga
     2761 gggtacgatc gttgacataa agatcttctc ccgcaagggt gctgagaagg acgagcgcgc
     2821 caaagcgatc gaggaggagc aggtttcgcg gctccagcgc aaccttcagg acgaagtccg
     2881 gatcctcaac gacgagcgcg ccaagcgcct gatggccctg cttgagggca agcaggtgct
     2941 ggccgatctg cacgacgagc gcaccaacaa gcggctcctt tcgaagggcg ccgagatcac
     3001 gcgcgacatc gtggaaaaac tgaaagcccg cgatttgaag cgcgtgcgtt tcaaggacaa
     3061 agatccgcgc ctcaacgagc agattgacga gatcgaagag atgacggtgc gtcagattca
     3121 ggttctggaa aaaatcacgg acgaaaagat cgccaagctc aagaaaggcg acgagctgcc
     3181 gccgggcgta atcaagatgg tgaaagtcta tattgccatg aaacggaagc tttcggtcgg
     3241 cgataagatg gccggacgcc acggcaataa gggggtgatc gcccggattc tgcctgagga
     3301 agacatgccc tatcttcccg atggaacgcc ggtggagatc gtgctcaacc ctctgggcgt
     3361 gccgtcgcgc atgaacgttg gccagatcct ggaaacgcac ttgggttggg cggccaaagc
     3421 gttgggagtc cacttcgcca cgccggtttt cgacggcgcc acggaacgcg agatcaaaca
     3481 gcagctttcc aaagcgggcc tgccgacctc aggcaagacg gttttgtacg acgggctaac
     3541 gggcaaccgg tttgagcagg aagtgaccgt aggctacatc tacatgctca agttgtcgca
     3601 tctggtggat gacaagatcc acgcgcgctc gatcggcccc tattcactca tcacccagca
     3661 gcctttgggc ggtaaggcgc agtttggcgg ccagcgcttc ggcgaaatgg aagtttgggc
     3721 gctggaagcc tacggcgccg cctacacctt gcaggagctg ctcacagcca agtccgacga
     3781 cgtttacgga cgcgccaaga tttacgaggc gattgtcaag ggcgaggcgg ctatggagcc
     3841 gggagttccg gaatcgttca acgtgctgat tcgcgagctt cagtcgcttt gtctggatgt
     3901 ggaattgatt aagaaacagc gggaaatggt ggatactgcg ctggccgctg attagcgact
     3961 cgggcggcag gcaggctttt cgggagcctg cgcctgccgc ccataacctg gttttaccaa
     4021 cggaggctta ccttgtaccg atcttctcct tatgatcgcg ccaacctgat cgcagacttt
     4081 gactcgattc gtatcagtct ggcgtctccg gaaaagattc gcagttggtc gcacggggag
     4141 gtgaccaagc cggagactat taattaccgc acgtttaagc cggagcgcga cggcttgttt
     4201 tgcgcccgaa tcttcggccc ggtgaccgat tgggagtgcc tctgcgggaa gttcaagcgg
     4261 atgaagcacc ggggcgtgat ctgcgacaaa tgcggcgttg aagttacgtt gagccgcgtg
     4321 cggcgcgagc gcctggggca catcgagctg gcctcgccct gctcgcatgt gtggtttttc
     4381 aaaggccttc ccagccgcat cggccacctg ctggacatta cgctgcggga gctggagcga
     4441 gttctttatt tcgaggctta cgtagtggtg gatcccggcg aggtggaggg cctttcgccc
     4501 ggcgaagtca tcacggacga gaagaagcgg cagctcgagc aggaacacag cggcaagttc
     4561 gtggccatga tgggcgccga gggcatcaag gaactgctga agcgcgtcaa tgttgacaag
     4621 ctgagcgaag agctccggga gaagatgaag accgagacct cgcttcagaa aaagctgaag
     4681 tacgccaagc gcctgaaggt ggtcgagtct ttccggaagt cgggcaacaa gccggagtgg
     4741 atgatactgg atgttcttcc cgtgatccct cccgagctga ggccgctggt gccgctcgac
     4801 ggcggccggt tcgccacctc ggatttaaac gacctctacc ggcgcgtgat taaccggaat
     4861 aaccggctca agaagctcat ggagctgcac gctcccgatg tgatcgtgcg caacgaaaag
     4921 cgcatgctcc aggaggcggt agacgcgctg ttcgataacg gccggcgcgg acgtgtgctc
     4981 cggggcgcca acaaccggcc cttgaaatcg ctgtccgaca cgctcaaggg caaacagggg
     5041 cgcttccgcc agaatctgct tggcaagcgc gtggattatt ccggccgctc ggtcatcgtg
     5101 gtcggccccg agctgaagct gaaccagtgc gggctgccca aaaagatggc cctcgagctc
     5161 ttcaagccct tcatctacca gcggcttgag cagcgcggcc actgcaccac gatcaagcag
     5221 gccaaggaga tggtggagca gcaggagccg atggtttggg acattctcga agaagtcatc
     5281 aaggaccatc cagtgctgct aaacagggct ccgacgctgc accgcctggg catccaggcc
     5341 ttcgagccgg tgcttgtgga aggcaaggcg atcaagatcc atccgctggt ctgtacggcc
     5401 ttcaacgcgg acttcgacgg cgaccagatg gcggtccacg ttccgctctc gccggaggcg
     5461 cagatcgagg cagccgtgtt gatgctcagc tccaacaaca ttctttctcc ggcccatggc
     5521 gctccgatcg cgattccttc gcaggacatg gtcctgggct gctattacct gaccaaagcc
     5581 cggcccgggg cgcggggcga aggccggacc tttgcttcga cggaggacgt tctgatcgcg
     5641 ctcgagatgg gcgaggtgga aacgctgact ccgatcaagc tgcgattcac gggaaaggtc
     5701 atcgacctgg tgcacgcctt cgacgatcaa aacatcattc acgccgagcc catcgagttc
     5761 aacaagcagt acatggaaac cacggtgggg cgggtgatct tcaatgacca tctgccgagc
     5821 ggcatgccgt ttatcaacgg tctgctcaag aagaagggac tcggccagct cgtgcagtat
     5881 tgctacctgc ggttcggctt gcaggccacg gtcaaaatgc tcgacgagct caagaccctg
     5941 ggattcctgt acgccaccca cgcgggaatt tccatcggca tcgacgacat ggtggtgccg
     6001 gccgagaagc agcagttgat cgaccaggct caaaaagagg tgattgacgt agaacagcaa
     6061 tacctcgacg gcgcgatcac ccatggcgag cgctacaaca agatcatcga gatctggtcg
     6121 aaggtgaccg aaaaagtttc cgatgagatg ttctcggcca tggagggcca ggacaagagc
     6181 ggcgagatca tcaatcccat ctacatcatg gcggactcgg gcgctcgcgg atccaagcag
     6241 cagatccgcc agctctcggg catgcgcggc ctcatggcca agccttcggg cgaaattatc
     6301 gagaccccga ttacggccaa ctttcgcgaa gggttgaacg tcttgcagta cttcatttcg
     6361 acccacggcg cccgcaaggg gttggccgac acggcgctga agaccgccga ctcgggctat
     6421 ctgacgcggc ggctggtcga tgtggcgcag gacgtgattg tctcggagcg cgactgcggt
     6481 acgaccgagg gcattttcgt cgagccgatc atcgagtcgg gcgaaatcat cgagccgctg
     6541 cgcgaccgca ttgtgggccg cgtttcgctc gaagaaatcc gcgactacga aggcaacctg
     6601 atcgtcggca taaaccagga gatcgacgaa gagctggcgg cggcgattca gcgggccggc
     6661 atcgagcggg tcaagatccg ttccgtgctg acttgcgaat caaagcgcgg cgtctgcgtg
     6721 atgtgctacg gccggaacct ggccagcggc cgcctagtcg agcgcggcga agcggtcggc
     6781 gtgatcgccg cccaatcgat cggcgagccc ggtacgcagc tcaccatgcg gacgttccac
     6841 atcggcggca cggcgacccg tgtttcggaa cagtccactc aggactccaa atcgaacggc
     6901 ttcgccaaat tcctgaatat cgtcaccgtc cgcaacaaga acaatgacct ggtggcgatg
     6961 aaccgcaacg gcattttggc catcgtagac gaaaaaggcc gcgagaagga gcgttacccg
     7021 gttgtatatg gggccaaggt gcgtgtcgaa gacggccagc cggtctccat caaccagatt
     7081 ctgctcgagt gggacccgta caccttctcg attctgaccg aagtgggcgg cgtcgtgcac
     7141 ttccgggact tgcaagaggg cgtcaccgtc caggagcagg tggacgagat cacgggcatg
     7201 tcgcagtggg tggtcacgga ctctccggat gaaaaacggc agccgaccat tgagatccga
     7261 agcgaaaacg gaaaggtgct gcgcaaatat ctgatgccga cccactcgca cctcatggtc
     7321 cgtgacgggg aagccgtgca tgcgggcgac gtgctggcga agattccgcg cgagaccacc
     7381 aaaaccaagg acatcacggg cggcctgccg cgcgtggtcg agcttttcga agcgcgcaag
     7441 ccccgcgaga cggcggtgat cgcggagatc gacggggtgg tgaagtacgg cgagctcgtc
     7501 aagggccagc ggaagatcga agtggtgccc gacagcggcg aaacgcgcgt atatacgctg
     7561 ccgcgcggcg tgcatatcaa tgtgcaggag ggtgagcggg tacaggcggg cgagccgctt
     7621 atggatggcc cgcgcaatcc gcatgacatc ctggcggtcc tgggcgagaa ggagttgcag
     7681 aaatatctgg tcaatgagat ccaggaggtg taccggctgc aaggcgtaaa catcaacgac
     7741 aagcatctcg aggtgatcgt gcgccagatg atgcgctgga ttaaggtcga agaagtcggc
     7801 gacacggact ttctgctcga agaagtggtg gacaagttca aattccgcga agaaaacgag
     7861 cgggtgatgg ccgcaggcgg cacgccggca gtggggcgcc ccttgctgat gggcatcacg
     7921 aaagcctccc tctcgaccga cagcttcatc agcgcggcca gcttccagga aaccacgcgg
     7981 gtgctcacgg aagccgcgat ctcgggcaag atcgattacc tgcggggcct gaaggaaaac
     8041 gtcatcatgg gccgcctgat tccggcaggc accgggatgc tgcattaccg gaatgtccag
     8101 gtgcccggcg agccggtgcc ggagccggag atcgaggaga ccgatgttct ggccggcatc
     8161 cccggttacg ctgaagaggc gcgggtggaa tacgccggcg ggctgtcgga agataattcg
     8221 gagggattgc ccgtcgaata gcgccggagc gagcggcggc cgcatagctg gtatcgccgc
     8281 ggcgcgggct taaatcgctc ggcgcggagg cggaatgttc gcagcttgac tccgccgacg
     8341 gcgagcacct acaatgaagc tcaggaggcc atatgccaaa tctcggcatg cccgaattac
     8401 ttgtgattct tggggtcgcg gtgctcctgt tcggcggccg caagattcct gaaatcggga
     8461 aagggctggg cgaaggaatt cgccagttta agaatgcact gaagggcgaa gaaaaagtcg
     8521 aggaaaagaa accgcaggca taggcttctg gcgcgccccg caccatggct cgcgccgatt
     8581 cgatttttct gaagttcaga ggcacaaaaa accccgtccc ttccctggct gggagaggac
     8641 ggggtttttg ttgcgttaac gccaagacct cacttcttct tgggaggcac gatcgcgtct
     8701 ttcgccgcct tcgcaacgcg aaactttacg accttcttgg cggggatctt gatcgcttcg
     8761 cccgtcgccg ggttccggcc catacgcgcc ttgcgatcta cgcgaaccaa ccgtccgatt
     8821 ccggggagca cgaacacgcc ggacttcttg gtctcccgaa tcgctacgtt ggccaactcg
     8881 tccaggaatt tccgcgctga cctgttattc acttcgcaag cttcggccaa cgtacgtacc
     8941 agttgggttt gggtcattct tcctgctgcc ataatcctcc ttgttgcttc tcctcttaga
     9001 acctttgaaa ggccgttttc aagccgcttt ggttaactac cagacattgc tccaacatag
     9061 taaggaaccg gcagcggaat gtaaagcgga aaatgcgaaa aatgggccgg agaggccgaa
     9121 aaatgcataa tagacaggga gggggaagcg gcgatggcgg tcattcgagt gatcggcggc
     9181 gggctggcag gatcagaggc tgcactccag atagcccggg cggggcatcg cgcgctgctt
     9241 ttcgagatgc ggccgctggc ctcgacgccg gtccaccgca cgggatgctt tggcgaactg
     9301 gtatgcagca actcgctgaa gtccgaacag ccggggaccg ctccctggct gctgaagcaa
     9361 gaactgcgcc ggctaggctc cgcattaatg gaggccgccg ggcgctgccg cattccgggg
     9421 ggccacgctc ttactgtgga ccgtgaggct ttcgccgccg aggtcacccg gcgggtcgag
     9481 gaggaaccca acatcgggat ccgccgtgag gaggcggccg agattcccgg cggtgacatc
     9541 accatagttg ccagcggccc gctcacaagc caaaggctcg ccacggccat aagccgcttc
     9601 accggccaag atcggctgta tttcttcgac agcatcagcc ccatcgtcga ggccgatagc
     9661 atcgattatt ccgtggtgtt ccgggcctca cgctacggta agtccctgga cggcacagag
     9721 gactatctga attgcccgtt cactcgggag caatacgacc ggttttacga cgcgctggtc
     9781 tcggccgagc ccgctgccgt ccacggcgag gaggacacgc cgtttttcga agcctgcctt
     9841 cccattgaag aattggcccg gcgcgggcgg gacacattac ggttcggccc gatgaagcct
     9901 gtggggctta ccgatccccg cacgggccgc accccctatg ccgtggtgca actgcggcag
     9961 gaaaacttgc gggcggagag ctataacctg gtaggctttc agaaccatct caaatacggt
    10021 gagcagaaac gggttttgcg gctgatcccg ggccttgaga acgccgagtt cacccgcttt
    10081 gggcaaatac accgcaacac ctatatcaac gcccctgcgc tgcttagccc gactctgaat
    10141 ctccgctcgg cgccgtggat tttttttgca gggcagattt cgggcgtgga gggctatgtg
    10201 gaggcgattg ccaccgggtt gatggccgcc cgccatgcga tttccctggc cgaggggaag
    10261 acgccggttc cgccgcctcg ggaaacggcg ctcggctcgc tggctcacta catttcgcac
    10321 gccgaccccc gcagttacca gccagccaat ataactttcg acctgttgcc ggctcttccg
    10381 gaagccgatc gttgcaatct cggccgcgac aagaaggccc gccgtgaact ccagtgcgcg
    10441 cgggcggccg aagcgctgga aaaatactgt gcggaatatg aagcactctg cgtccggtga
    10501 actgagctcc gcaatcgagc ggttcctgcg gagcctcgcc gtggctaacg tgtcgcccca
    10561 caccctgcgc aactatgctt ccgacttgaa gcagttcgcc gaataccttt ccccgccggg
    10621 gagcgctgcg cctgccttga aggccatcga ccatctccac atccgggaat tcctcggagc
    10681 gctctaccag cgcaagaaca gccggcggac ggtagcccgc aaactggcgg ctctgcgggc
    10741 gttttttcgc tttctcacgc gggagggcag cctggccgcc aatcccgccc gtctggcaac
    10801 gacgccgaaa acacccaaag cgttgccgcc cgtcctcagc gccgaacagg cgaatagtct
    10861 ggtcgatgcc gccggccgcg aggggagccc gcgcgatcgg gtaattttcg agttgctgta
    10921 cggctgcggt ttgcgcgtat cggaactggc tgggctcaat ctggatgatt tcgattggag
    10981 ccagcggtgg atccgggtcc gcggcaaagg acgtaaggag cgcctcgtac cgtttggaac
    11041 caaggcggcg gaggctctcg agcggtacat gcaagggcgc gcaggctgcg gcggccaagc
    11101 gctgcttctc aatcctcgcg ggggccgctt gaccagccgc agcatcgagc gcatcgtgaa
    11161 gcggtacgcg attttgtttt cagccgatcc gtcgctgcac ccgcattctc ttcgccacgc
    11221 attcgctacg cacctgctga gcgacggcgc cgatttgcgg gccatccagg aactgctcgg
    11281 ccacgccagc ctttccacca cgcagaagta cacgcagctc tcgctcaaag aattgatgga
    11341 agtctacgat cgggcgcatc ccagggcgtg agaccggaat tgctgttcac ctcggcgaaa
    11401 gggccttgcc ggccagtatc tccgcgatcc gctcgcaagc atggccgtcg ccataagggt
    11461 tgtgccggcg cgccatccgc cggtagcgtc cggcgtcgtc aagcagggca ttggtctcag
    11521 tgacgatggt ctccacgttg gttcccacca ggcgcaccgt tcccgcccgc acggcttccg
    11581 ggcgttctgt tttctcccgc atgaccagta cgggctttcc gagcgacggg gcttcctcct
    11641 ggacgcctcc ggagtcggta agaatcaggt agctccgggc catcagatcg acgaaaggga
    11701 cgtactccag cggctcgatc aggtgaatcc ggggccggct cgacaagcgg ctgtgaacgg
    11761 gtccttgcac gcggggattg caatgtacgg gatagacgac gtcgatgtcc gggcgctcgg
    11821 aaagctttac cagcgcctcg cagattcttt caagaccctc tccaaagctc tcccgccggt
    11881 gggccgtaac cagaatcagc ctgcgaccgc gtgcaagaaa gggccagtcg gggcgaagca
    11941 agcggccgct ctccaagccc cggcggattt cgagcaccgc gtcaatcacg gtattcccag
    12001 tgacgaaaat ggacgcggcg ggtatgccct cggcccgcag attggccgcg gcctgcctgg
    12061 tcgcggcgaa gtgcaggtcg ctgaggcggc tggtcaagag ccggttgatc tcttccgggt
    12121 agggctgata cttatccccc gtccgcagtc cggcttccac gtgcgcgatt cgaatgccgg
    12181 catagaaccc ggccagtgcg gcgcagaagg tggtggtggt atcgccctgg acgattaccg
    12241 tatcgggttt ctcgagattg agaaccggtt cgagcgccgc catggcccgc gaggtcagtt
    12301 gcgggagggt ctggtcgggc cgcatgacgt cgagatcgta gtccggctgc acttgaaagg
    12361 cttggaggac ctgatcgagc atcgagcgat gctgcgccgt gacgcaaatg cgcacctgga
    12421 atgactctgg acgttctcgg agccgccgga ttaacgggca aagcttgatc gcttccggcc
    12481 gcgttccgaa tacaacgaga acgcgggcgg gacgggacag ggccgcactt aaaggcatgg
    12541 ctcagttcag catggccgtg agcctcttca actcggcgat cgtttgctga atggcctcgg
    12601 catccgcgct ttgcggattc aaaaaaaggc aggcttcgag atcttgtctg gcctgcttgt
    12661 aatggccgag tttatagtga atcaggcccc gctgtttgag atcatcgctc gaggccggcg
    12721 taatcgcgag aatcatctct atgacacgca aggccttcga gaactgctgg ccgtgcacgt
    12781 aaatgctccg caggttgttg agcattctga gaacgatata tcgcttgtcc accggctgca
    12841 gaaaactcgg atggaaagag agccggccct cgtacatgcg cctcaccagg tcgcgacagc
    12901 cgctcgaatc gacaaagcgg ccgccgtgga aggggtcgat gaacaatgcg tcatcgttag
    12961 agtcgtagta tttcaacaga aaatgaccgg gcaagcctac gccagccaaa ggcgctccca
    13021 gtcgccttcc gacttccata tatagaaccg aaagagttat ggggatgccc acccggcgat
    13081 ccagcacgtg attgaggcag ctgttttttg ggtcgtagta atcttgctca ttgccccgaa
    13141 acccgcaggt ctcagccagg aacagccgga gcgcctttac tctgtcaagg ctcgctttgt
    13201 ggatggaaag cggcaaacgg gcggcttccg ccaatgcttc caggcgagcg agttccctct
    13261 ccacatccaa tcccgggcac tcgcaacgag ccagcaaaag ggcggcgcgg ccgaggtcaa
    13321 tctggtcgtc cggctgatcc aggacctgga gcaatgttcg ctcgtctctc atggctccgt
    13381 accagtgtag caggcttgtt tggcggggct gccctatgac cgcagggagg gaaaagacct
    13441 agaatcaatt tttcattgac atttcagcta cttataagat aagttcgaag agtcacgtta
    13501 gcggtcttcc cgcgggccgc atgagcggat agggtacagt gagcgctaat cgaccttttg
    13561 ggcgaggcca aacaagatga tttttctttc cctgttgatg aatttgatgt tgatgttctc
    13621 taccggcgta gggctgaaca tggcgacgcc taacgccgtt ctcgacccgc agatcagcgg
    13681 cgagcatcgc acgcacgggc atctgccggg gccgccggac gggcacaacg gcgactctca
    13741 tctgcatcac gggcaacacg ggcactccgt aggtcctagc ggtgggggag accatctgcc
    13801 gccggggcat actaacacct gatagtttcc acggacggca gcgaatctgg ctgccgttca
    13861 aagcttcggt tctgaccggg ggctttccgg gcctccgctg tcgtgcgccg cccagtgtgg
    13921 aaggctgttc ccggctccag caactccgcc tgtcttggaa tcggtctccc ggtgtgttcc
    13981 cggttccggc gctcagtgca gcgcgccgat ctggtaggaa agcaccgagc gctggatctg
    14041 gtaatcatat ttagcttcgg cgctggcgat ctgggctgac gttttgttca actgcgcctg
    14101 actcagttcg acgatcgagc cgagtccgag atcgtagcgg gcttgagcga gatcctgagc
    14161 cagtgtggct tgctggagca gttcgtcggt cacgctcaac cgctggtagg cggtattcgc
    14221 gttcagccaa gcaaccctta tgtcgcgagc gatcgcattt tcgaggtcgc ggacctgctg
    14281 ctcctgggcc tgcgcccgca ggtcggcctc cgtgcggcgc gccgtgaaca gatgcccgtt
    14341 gaagatcgga atgttgacgt tcaccccggc ggcgctgtag tgatcggtga tgaacgagtc
    14401 atgcgctggc gagccgcctg ccactccgac ggcgctaatg gtgggcctgg caagatcccg
    14461 ctcggctgcc gcgaacttct gggccgcgtc gcggcttaag cgggaactgg ccagttccgg
    14521 ccggtcgcga aggccttgcg ctaccagatc cgccagcgaa gcaggcgggg ccccgggcat
    14581 gggctcttcg gcaagttcgt aagtctgctc atcctgaaag cccagagcgg ccgtaagctg
    14641 cgcaaaggca gcccttattt cattctgcgc gtcgatcagc agcagccgtg cttcggaaag
    14701 attcacccgg gcgaagctca cgtcgagatc ggacttcagt ttgctttgcg ccagggctgt
    14761 aacctggtcg gcgacgagct ggcgggcgct gacggtctgc tgggctactg tcaggacggc
    14821 ctgggcccgc aaaacagcga aataggcccg atcggcctgg agcagaattt gcgcgcgggt
    14881 ggcttgcgcg gtctgctctt ccgcctgcgc gcgaagccgg gcgttctcgg ccaggttgct
    14941 cgttcggccg aaatcggtga cgagctggtt gatcgcgaag ccgccggcga accggttgaa
    15001 tatcgacgga ttgttgagca atccggcggt gatgcggctg tctttggccg cgcctgcccc
    15061 tgtcaggctg ccgaacaccg tgggaaagaa ggccgaacgc gtctcgatag tgacctgatt
    15121 cgcggcgagt gcggtcagaa acgcggcgct cacctgcgga tgatttttgg ctgccaactc
    15181 ctccgcttgc cggagcgtga gcttccgcgg gggagcttgt gccgatccgc gcgtcaggcc
    15241 taatgtgaca gctcccagaa cgagccaggg ccagactcga tttctcattg cgcggccccc
    15301 tgagggtgct cagcgcccgg ggcggcatcc tggcggcgat aaaccagata gtagccggcc
    15361 ggcacgagaa tcacggtaaa taccaccgaa atagccaacc cgccgataat ggcgcgtgcc
    15421 agcggcgcgt aagcttcgct tccagcgccc agtttcatgg ccatcggcag caatccgatg
    15481 attgtcgcca gggaggtcat cagcacaggc cgcagccgga cgcggcaggc aagcgccact
    15541 gcggctggga cggtcattcc atcggctcgc aagcggcggg tgaactcaac gatcaggatg
    15601 ctgttggaaa ccgcgattcc taccagcatg actacgccca tgagagacat aacattcagc
    15661 gtggtatccg taagatatag agtccagatt acgccggtga tgctcggcgg cactgccaga
    15721 agaataataa agggatcgat gaaagaagcg aactgcgcca ccaggatcag gtacagcaaa
    15781 accacggcca gagtgaggcc caggccgaaa ctccggaaag aggcgcgcat gccttgcacc
    15841 atgccgcgca gagtaaccga agtgccgtcc ggaatcttca tgtgagaaat tgtggcgtcg
    15901 atggcgtcgg cgatcttccc gaggtcttca ctgaggggcc tgacaaaaac gtcgatagtg
    15961 cggcggagtt ggtaatgatc gacttcggtc ggtgaattca cccgcgtgat cttgctgacc
    16021 atatccaggc gagtcgggtt ggcggagatt ctcccatgca gcggaattgc gcggaggtcg
    16081 agcaggctgc cgatttgcag ctcgggatac tgcaccgtca gcatgtagtc gttgccggtt
    16141 ttggggtcaa tccaatagct tggcgcaatc atgccgttcg aggtaagcgc ggtaattacg
    16201 ttgtccacaa tctcccgctg gctcagaccc agttcggcgg cgcgcgtacg gtcgatgtcg
    16261 aggtgaaggg ccggataatc caaatcctgc gggatgtaca cgccggccac gttgggaatg
    16321 tttttgatgc gggaggcaag cgagagcgcg gtttgatagc tccgctcgag atcggctccg
    16381 gcgacctgga catcaatcgg ggccggaagg ccgagattca gcacagcgtc cacgagaccg
    16441 ccagattgaa aataggtcgt cagttcgggc agttcggccg ccatcgcctt ctcgacgcgc
    16501 tccatgtact cgtagctgcc cactttgtgc ccatccttca ggctcacctg aacaaatgcc
    16561 gtgtgcatgg ctgagttcgg cgtataaatc gccgaaaaat cgggcgtgac gccgatgttg
    16621 gaaacgatca gcccgaggtc ttcaggcgaa acaattttgc ggatcagagc ctcgactttt
    16681 gcgatttcgc tctccgtcac cgccaggcgg gtgccggatg gcgctttgag gttgattaca
    16741 aactggcctg cgtcggtgcg cgggaaaaag gccagaccga gccgcggcca gagagtaaag
    16801 ctcacggcga aaacggcggc gaaagccgcc agcacgaagg cgggccgctt cagggtggct
    16861 ctgacagcct tgtcgtaacc cttcagagta ttctcgaaat gacggttaaa ccagagatta
    16921 aaccgctctc cgaagctgcg gggggccggt gtttcctcct ccccggccgc ctcctccgca
    16981 tgatgcccgt ggggcctttt gataaagaag gcgcaaaaca ggggaactac cgtcatcgcc
    17041 acggcgaatg acgcaaaaag cgacagcaca accgccaggc ccagggccga gaaaagaaac
    17101 ttgctcacgc cgtaaagaaa tgttacggga aaaaaaacga caaccgtagt cagcgtagcc
    17161 gccagcaccg gcaaggccac ctcccggccg ccgttttcgg ccgccacgcg aggcggctcg
    17221 cccatttcga gatgccggta aatattttcc agaaccacca ccgagttgtc gatcaagcgc
    17281 gaaaaggcga gagccagccc gccaagcacc atgctgttga tggacccgtt tcccagccat
    17341 agcgcgatga acgtcgccag cgccgacagc ggaatcgaaa agaagacggc gatggtggcg
    17401 cgcatgctgc ccaggaaaat gagaatcatc aaagaggtca gcagcagccc gattgcgcct
    17461 tcgtgcagca gcgtttcgat agccgttttc acgaattccg attgatcgaa gacaaccttg
    17521 gttaccagct ccttgggaac gtcgaagagg tgcgcgacag tctgctttac cccgtcgact
    17581 acctgaatcg tgttggtgtc gccgccttgt ttcaagatgg gcaggtaaac ggagcgttgg
    17641 ccgttcaccc gaaccagatt cgtttggatc tgctgggcgt ctttcgcctc tccaatatca
    17701 gaaacgcgaa ccggattctg cccgattgtt ttcagcggaa gatcgttgat gtcttttacg
    17761 gtgctaagct ggctgttggt ataaatgttg taatcgatcg ggcccagctg cacgtcgccg
    17821 gccggcaaga tcagattggc gtcgttgacg gcgcgcacca cgtccatcaa gctgagctgg
    17881 tgggcctcta atttgtaggg gtcggcgtag accatgatct gccggtagag tcccccgaag
    17941 ggctgcggga cggaagctcc aggcacgctg gcgatttgat ttcgcacgtt gaactgggcc
    18001 aggtcgcgga gagaagtctc attcagcccc tgcccctcga aagtaaccag gcaaaccggc
    18061 aagctggaag catcgaactt cagcactacc gggggaagtg tgccgggagg cagccgccgc
    18121 aactgagcca tcgccagatt agaaatggtg gtgaccgcag aatcgggatt cgttcccggt
    18181 tggaagtaga ccttgatgac gcttacgccc gtcagcgagc gcgactcgat gtggtcgatg
    18241 ccgctgccga gcgtgaagaa gcgttcgaaa cggctggtga tgtcggtctc aatctgttcc
    18301 ggcggcatgc ccgaataaaa tgtcgccacg accaccacgg gaatgttcat cgccggaaac
    18361 atgtccacgg gcatccgggt gatgctggcg acgccgacta cggcgatcac cagacacacg
    18421 acgacaataa aatatggggt cttgattgcg aagcgtgaca cagctactgc ccttcttctt
    18481 tcgccgcagc catctctacc agcttgggac tgacttgctg gccgggatgg atctggttgc
    18541 ggctgccgat gacgaccaga tccccttccg acagcccggc gcggacttcc acgcgatttg
    18601 cggtttccag ccccagggtc accgcgcgct cctcgacagt accatcggga cgaactaaga
    18661 aaacggtcgg cttttgatcg ctgccgccga tcgcagtgag cggaatcgcc agcgcgtcat
    18721 tgcgctgctg gagcgtcagg ttcacggtgg cgtacatgcc gggaatcagc acgagatccg
    18781 gattcggcac gtctacttcg gtgtccatgg tgcgcgtgga aaggttcaac ttgtccgcaa
    18841 accgcaccac ttttccggga aacgtccggt tcagcgaagg cactttgacc tcgacgggct
    18901 ggccgacgtg gacggtgggc acggcagact caggcactgg aagaatcagc cgcaagagac
    18961 tgttttggga caaccgcacg acgggcatgg cctgcgtttg cgaggcggtg ccggcctgga
    19021 tcatcgagcc ggtgtccgca taccgtttgg tgaccacgcc ggtgaagggc gcggtgacct
    19081 gggtgtattc gaagatcgtc ttggtcctgg cctgctcggc ctcggccact cgtacctgct
    19141 cctcagcggc ggcaagagct gatttcgcgg cttccacctg cgcctcggcc accagatcgc
    19201 ggctgcgggc gtcatcgact tcctgctgag ccaccaggcc cggccgcgtt ttcataacgc
    19261 cggcgaggcg gtcggccgtc agatgggcga tttcatgggc cgagttggcc cgccgcagtt
    19321 cttgcttggc gcgctcgagc tccgctgtat tgcgtttcac ggccgccgcg gcgcgggcca
    19381 ggtcgtcggc catttccggg acttcgagcg tagcaagcag ttggccttcc cgaacccggt
    19441 cgccgatatc gacgttgatc tgctttacgt accccgcgac cttggccatt acatctactt
    19501 cctggtacgg ccggaattcc gcggtcaaaa ccaggtcgcg ggccagatcg gctctttcgc
    19561 atttcgcgac cgcgacgatg agcgcttttg gcggatcggc tgtccctgct tcggtcttgg
    19621 cgggccgcga gcaggaagag acgatcactg cgaaaacgat cgccaaaccg gcggcaactc
    19681 ctgtatacac tctggttaac atgcaagcct cagctatccc agttctttga tgatttcccg
    19741 caatttttcc agagaaaatg tgggggaccg gcatagtccg atcaccctct gctctctagc
    19801 cagcatgcgc gctgccacag caggcagatc ggcggcaatc tccctgggta tggataagat
    19861 tccgtggcag tctccgaaaa tcagatctcc ggagcggatc gtcagccctc cgatctcgac
    19921 cggctcgccg aactcgacca tgtgcacgta agcatgggac accgacggat gagccgcaaa
    19981 gagttgatac cccattttct cgacagccgg caggtcgcga acgcccccgt tcgttaccgt
    20041 tcccacgcag ccgagagccc ggtgaatgct tgcatgcact tcgccgacaa agccgccgac
    20101 gccaggcgga tcatcaaggt cctgaacgac tgtgacgcgc ggccccggta cggtcaggat
    20161 atagctccac cagtcgccgc ggtcgaaata cgaatggccg accggcgggg ggccggagcc
    20221 gcggatgcgg gcggtgaccg cgtagccgag cattggtgga agctgcggga acatacagcg
    20281 gatgctggcg tcggcgaagc cctcgttgcg caggcgcaga tcgaaagtct cgatggcgtt
    20341 cgacagagtg caggcgtcga ggcgccgaat ggcttctaat tgatcaaagc tcaaaattga
    20401 ttccacacct tgctcccttc ggcaaggatt ttcgtgaatg aagactggga acgtaaaaac
    20461 gaaaaccgca ggataactag caagccgccg gcggggggcg aaactcaaaa ggccggacca
    20521 gggagaaaaa cctgggaagg actacttctc tcggggcaac gcaaaactcc cgggccgagt
    20581 cgtcggcgcg gatttccacg gtcttttcgg caaagggagc ctcgacccct tgggcgcggc
    20641 attccttcat cttggccgca ttggagaggt atttagtcgg accgtcttgg tgatagagac
    20701 tcagcttcgc ctgggtggaa agagcgaaca ggagcccggc gagcagcaga atgcccgcgc
    20761 gcgagaaccg gaacgccaac ccagctcgtg gcatactggc ctgactatac atccgcgctc
    20821 gatctcctgt caattcggcg cggcgtcggg gcgaaaacat ttctgacatt aaacgttggc
    20881 gaggtcgatc ccgtatttct tcatttcctc gtcgtaatag cggcgaaaga gcaattccca
    20941 ctcctcgctg ccctcgggga tctcgcgctt ctgggacttg attttgacgc gggcggcatg
    21001 gtcgatctta tcttctacga ccagcaattc ggtgagtccc ctcacgatct ccagccggac
    21061 gtcgttccac tccattttga acttcacccg gggaagccgg cgcaacagag cggccagctt
    21121 gtgcgacagc tcgttgatct tgtcccgcga aagcttcgtc ggatcgcccg tctctcggcc
    21181 cgaagcgcga atgatcttcc gctcggagag gagcagccgc ttggtccgct tgaacatttc
    21241 ctggtagctg acgccttccc ggcgcatgta atcgctgtac tgccccagaa tttcgcgcac
    21301 ctcgtcgttg atctggtctt cgacggaaag ctcgtcgatg ataagattct ggaccgcctc
    21361 ccggacggcc tccggatctg cgatttccaa cggaccgtcg cccagacgct tcgtgaggtg
    21421 ccgggccaaa taagaaataa acgccctgga caacagcatg tttgcattca gtttagcata
    21481 gagatccggc ggcctacgct tagccgctgc cgggcggccg gcagatggaa tccgttccca
    21541 ggccgcggcg ccctcgagat caaaaagcga ttctcccctc gggcggccga cgaacgccta
    21601 tcgcagcgcc gccaccagag ttcccgtttc tcgcaggaga ttgagccggg ccttggcggc
    21661 ggcggtctgg gcgtcgtaaa acgcttccca tttttgcgat tccgccacgc gcgcctgttc
    21721 cacttcgttc atactgatgc gtccctcatc taagcgcgcc agatagaccg agagagtttc
    21781 tcgagcgagg tccagctcca tgcgcgccag atcacgggcc gtttccgcct gccggacttc
    21841 ccgaaacagc cgccgcgttt ccaccgccag gttcgactgg gcggctgaga tttgcaactt
    21901 gacctgcgat atttccgact gcaccgccgc cagacgcgca ctgatctggc tgcgggcgag
    21961 aagtggaacc tggaaggaca ttccaagttg gccgttgttt ctctggaatg ttttgaaaaa
    22021 ctcttcgtaa ttgttataac ggcccaacat ggcgtattgg gccaccagat cgattctcgg
    22081 gaaataggcc cctttctcgg cttttacgtc gaattccttg gctcgcaggg tggcttggag
    22141 gcgcttcagc tcgggactgt tggccagcgc atcggcgacc gcagattctt cggtctccgg
    22201 taagacggtc tccgcgggca gcccgctctc gacgggtgcg atccgcacgt tctcatctaa
    22261 tccaagatcc gcccgcagca tgtcttccag gaattcggcc cgggcctgga ggttttcgac
    22321 ttgagcgcgg gcccgtgccg tttccacccg ggcgcgagtc aggtcgagcg ggatgaggcg
    22381 cccttcctcc acccgggccc gcactaggcc ctcaatgcgc gtgaccagat ccaactggct
    22441 tctggctgcg gccgccgcgc gggtggcgcg ctcgaagtcc aaataattcg cggccacacg
    22501 gtacgcaatc tcctcggctt tgccttccct ggaccggctg gcggcggtcg ccatttccgc
    22561 cgcttcgcgg acgcgcatgg actgcgggcg gttgtaaatg aacttggatc catacgcttg
    22621 gaaaatcgac ggcgccgagc cggagatgga gagcggaaag ccgttgctgt aagccaagcc
    22681 ggagccgacc gcgatctggg gaatgaaggg cgctcgggct tctgcgatcg cctggcgcga
    22741 tctctgctcc tccaggcggg cgagcagata atcggggttc tggcgcaatg ccagagctac
    22801 cgcctgttgc agggtcaaag cgcgcgtctc cccatcggcg ggagcctgcg cccaaaccgt
    22861 ggcggcggcc agcaaagaaa caggcagggt tgcagccaat cttttcgcgg ttttattcat
    22921 atctcagagc gtccgttggg ctgaggcgcg cggcgcggga ggccggcagc aggccgaaca
    22981 ccacgcccac gccgcaggaa accagcaggg cggcgacgat cgcgacagcc gatatcggga
    23041 tctccacgcc ctctaggaag tgacgggcca cgagcggcgc cgaaacgccg accagaatgc
    23101 cggcgacgcc gcccacaaag ctgatcgcgc cggcttccgt caggaattga atcagcacct
    23161 gccgccgcgt cgcgcccagc gccatgcgaa tgccgatttc tcgcgtgcgc tcggtgacgg
    23221 tgaccagcat gatattcata ataaatatcc cgctgatggt caacgcgatc gccgatacca
    23281 ggaagagcac gatggtgaga gcgaaggaaa tccgccgcgc tgtgctcaaa agagccgcca
    23341 ggttgttcac cgtgtaaagc gacccgggcc ggtgccggct ggtcagaatc tgccggacca
    23401 ggcgcgtggt cgcttctacc tcgtcgggag agcggacgga gacatacagc ggatccacgc
    23461 ggggacgctc ggtgaagtac tccatcaccg acatcgggat gagcaccgat tcgcccgata
    23521 tttccgattg gccgaaggtg ggaatgcctt cccggaaggc gccgatgatg gtgaattgca
    23581 ggccgtgaac cttgatggag ccgccgatgg cattctgggg cgatccaaaa agccgggtcg
    23641 ccagatccgt tgtcaggagg cataccttgt cccggtcccg tatttccgca tcgtcataga
    23701 aacgccccgc cactacggtc aggtggcgga caatcttgta atcggtatcc gagccgagga
    23761 cggccacatc gcgctggcgg ctgtcgagca gcatgcgatc gtagatcttg atcacgccag
    23821 ccacgcccga cgccgcgtct cccagttctt cgcgcactgc ggccacatcg tcgggcgtga
    23881 tgtagtcgct ctgcatgcct ccggccacgt tttctccggg ctcgtaatac gcataaatca
    23941 ggttggaccc gacgccctcg atcaagcgca cgacaaacgc gcggccggcg agggccacgg
    24001 tggcaaccag aatgaccgac gcgttgccga tgaccatgcc gaggccggtc agaaaagtcc
    24061 tcagcttatg cgcccagagc gccgtgaagg cgaatcggat tgtgtcgagc agcatcaaaa
    24121 tgccattctg cgccggaaaa attcagccgg cggggtaggc cgccttttgc cggcgctcgc
    24181 cggcgccgat cggcctcggc gaagttgtgt ccccttgccg gatttggccg ggtcgggggc
    24241 ggtactaaaa aaatcagggt gcgcggctcg ccccaaaaca gagttgccgg agcggcgcgg
    24301 ttccggggcc cggataagac ggccagcgac gctttcaccg gcccgcgcct caacccgaaa
    24361 ttcgacggaa cactgagccg ccccaaaagc cgtctgtaat taccgcaacg gcgccatcta
    24421 aaccgctccg cgcaggaagc caagatcggg gccggaagct aaagcagttt cttcagcagc
    24481 ccttgcacct ccgaaggggg cggatcttcc ggcttgcggt tcgagcgcag ataggcttgc
    24541 agcaactggc gggcccggat gggatctcgc tttgaggcga ccagttgctg gcctcgcgtg
    24601 aacaggtaat cgggcgagtc gggcgccagc ttctcagcct gatcgaatag cgcgtccgcc
    24661 tcggcgtacc tgtggcgctc gttcaggaac gaagccagtt ctaaaagcct gccaggattg
    24721 gcgggttcca gcgccagcgc tgcccggtat tctcgttccg ctcctgcatg gtctttctcc
    24781 gccgcgcgga tcaaagcctg ggcgcggtgg ccctcggccg cattgagggt ggctagtttt
    24841 gcggcgaccg ctttcgcccg gtccaggcct ccgccgagaa aaccgggggc ttgtaaatca
    24901 tagtccagga ggtcgctcca agcttcgata ttcgttggat ccagttcagc cgcgcgggca
    24961 aattcgtttc gcgcctttct cgccagcacg atggcgcgaa taaagatcac gcgctccgcc
    25021 cggcggccat aggccttgcc cagccagtcg tgatagcgcg agacgccagg cgccgcctgg
    25081 acggcctttt ccagaaaatc gatagcggct tcgtaatttt cggctccgta ggcttgcacg
    25141 cctcgctgaa acagcgattc ggcggccgcg ttttccaccg ggtttttcgc cccttctttt
    25201 acgtccggct gtttgatgcg gggatccaaa gccggcggat cgggttccgc ttcccgcgtt
    25261 tttgcgggcg cttccttggc ctcttcggcc tcccggggcg gtgttccggt gtttggattt
    25321 tgcgcctgaa gcgcagtcca ggaggagaat gccagcaacc ctagcagcag tacgttcatg
    25381 tggtgatttt ggggcgaacc tgctgccccg gcgaatagat atgctcgccc ggcagaagca
    25441 caatctggtt gtcggccaat ccctccagca cctggacccg tcccaggtca tgaataccca
    25501 gccggacggc ctgccgcgag accataccgt tcgcatccac taccaaagcc atggtcttgt
    25561 caccctcccg caccagcgcc tcgcgcggaa tggtgagggc attccgggcc tctccggtcc
    25621 ggatggcgac gtttacggtc acattgggaa gcagccggcg ctcccgcgta tcaatcgtgc
    25681 agagcaactc ccctacgtta cgcgttccga cggtctgaat cagtgagggc aagcgttcca
    25741 cgctgccttc ccattccact cccggcaacg cgtcccaagt gatacgcacg ggctggccga
    25801 tccggacggg gcccagctcc ggctcgtcca ccagcagccg gacccgaacc ttatccagca
    25861 gaccgacctt ggccacaacc gcgccgggag cgtaatagcc gcccggccgc agcgcgagca
    25921 tatagagtgt gccggatatg ggagcacgca cttccgtctg gctgagggct gtcatagcct
    25981 gggccacggc cgcctgggcc tcgcgaacgc gggcctggat gcgctcgcga tcctcggggc
    26041 cgaggaagga tttgcgcttc cgctcgaggg ccgccatatc catctcggcc tttatcaagc
    26101 ggcgttgctg ctcctgcaac tcgacgcgcg gagcggcgcc tttctcaacc aaacgctgga
    26161 ggacggcgat ctctttctcg gcggtttccc tttcgaggcg ggcttcggcg atggcagaat
    26221 cgatctgcgc gatctccgcc gagctgccgc cgcgctcgag cacggcctca tcggcgcgcg
    26281 ccgcttccag ttgcgcctgg gcgtgcgcca gggcttcccg cgcggccgca gtgtcaactt
    26341 cagccagcag ttggccgcgg cgcacctggt cgccctcttg aacgttcacc ttgatgacgc
    26401 tgagcccggc ccgcgcgctg acgtccacgc tatcgaaggc ttccactttg ccgttggtgg
    26461 tgagagtctg gacaagatcg ccgcgtttta cccgcgcggc aggcgcttcg gggggttcgg
    26521 gctggcggaa gtggagccag agcgcaacgc ccagcgccac gagaacgaga gtgagggcgg
    26581 cgcgcttcaa tttggcttcc ggtaggcgaa gtcccagact tgaatattca ctctcgcgct
    26641 gtcctgcaaa tcctgaccgc tggatgcaaa ctgcactacc gcatactcac cgggttcgat
    26701 cgggtcgacg ggccaaatct tgtagacggt cggggcaagc tgctgccgga agacctccac
    26761 ctctttctgc tgttccatca cttccttgct gacggggata atggtgaggt cctctacgat
    26821 gcgcgaatct ttcttgacgg tgagccgtgc gatgccgagg cgctccacac ggtcgagacg
    26881 cacgtaaaat tccggctttg gagaattgac cacgaacgcc gaatgcggcc cttccagctc
    26941 gaccgtagct ttcccggcga ccacaggaat cggcgcaatg accttgagga ccgagcggcg
    27001 tttgttccct ttgactttgg gatccccttg cttgagaggc gttgtctgat cgccgttcac
    27061 ccaataaacg ccgtcgtcta gcggaaccct ctgcaattcc gtgcggaacc ggcgttccgc
    27121 ttccctctct gcccggtctt cctcagcccg ctgctgctgc tcctcctcta tccgcttccg
    27181 ctcgcgctcc gtgcggtcca gatccaccaa aaccagcggc agctcctccc actggctccg
    27241 ctccacgctg taaaatcgaa cgcggtcgga aagcacctgg tattctctca ccaggtggtc
    27301 agatccgtct ttcaggtaca atcggaagtt ccccgcggcc agcgccgcga tggccgaaag
    27361 tatgcctaaa atgaaggttc ctacaatccg catgccgttc caagcgccgt tggacgcatt
    27421 ctccggccct ttcgttgccc tttaacagta tagagcatcc ggccgtgcag gaccgcttgt
    27481 gcggcgcggc ggcggaattg tagcctggag gtaatgactc ccgccacgca tgtggtcgcc
    27541 ggcgcagcag ccgcggcgcg gttccgaaac gccggcgcag ctctggcggc cgccttcctc
    27601 ctgcatttct tcctggatgc tgtttaccat tttgaagcct tttacgagct ttcggttccg
    27661 gcgcagtgga gttacagcta cacaatgttc atgctgttcg gggggctgct gcttctcgga
    27721 gcgcctgttt tcgtgcatct cgcgcgccat gatcccgccg tttcgtcgtt cggctgctac
    27781 gccttgttga tgtgcgccct ggccttcgag ccgaagtggg actggaaact gttctgggga
    27841 gccatgttgt ccgcgttttg gctgctgatc acccgggccc cggagcctcg caaatggatt
    27901 ctctgcggct ttgtctccta tttgccggac tgcctgaaaa agccgatccc gctgctggcc
    27961 aggctgcacg acgccgcaca ttaccgctcc gatctggatc tgggggactg ggtttcactg
    28021 ctgggacgcg ggcgctggcg gattccggta aacgacagaa ttttcgatcc gtattatcaa
    28081 gctggatatg tgctcgaaat tctgctcgaa gcggtgatcc tgtttggatg cctctactgg
    28141 ctctcccgcg ggagggctgc ggcaaccgat cacccggccg gccgccagag cgacgccatt
    28201 gcggagggaa gcctgatacg aaaataggcg ccttgcgggc tccggcgctg ccgaagacga
    28261 attccccaac ataatgcgga ggcggcagat tcgtatcggc ttgccgtatg acggcttgcc
    28321 ccgaattatt ggacctttgt aacttcgaac ttcccgatct tttccagagc cggcgcaatc
    28381 ttttgcgcat ccccgaccac gacaatcgcg gcttgctcgg gcgaaatgta tttcttcgcc
    28441 gcgctcagaa tctgatccgg ctcgacggag cggatgcggg tggtgtaagt ctccagataa
    28501 tcattaggca agcccatgct tttgaccaac gccaactgcg tggcaagccc gttctggctt
    28561 tcgaggcgca tgacaaagaa gccgctcagg aagtttttaa agtcgcgcaa ctcggagtcg
    28621 cccaccggcg ttttggacat gacctccagg ttgctcagca tcgcttccac cgccggctcg
    28681 atcacttcat tgcggacctg ggtgacagct tcgaatacgc cggcgtcctt ctgggcatca
    28741 agaacactgt gggcatcgta agcgtagccg cgcttctccc ggatgtcatt gaagagccgg
    28801 gagctcgttc ccgtgcctaa aatggcgttg gccagcacca tcgggaagta ttccgggctc
    28861 gtgcgggtaa ccgccagccg gccaacacga atatcggcct ggacggagcc tggacgatca
    28921 accagtacaa gcgtgcgccg gctttcgggg aactccgcct taggcggggc agggaggggt
    28981 ttttgccgcc attccccgaa cttctgccgc accaggtcca tgactttgtc tcgctgcggc
    29041 agttggccca gcacaatgag tacggcgttg tttggaacca gaaattgatc gcgaaaggcc
    29101 gtaagctttg cggcgtcgat tttgtcaatc gactcgggcg tagggctgat ccgcgaataa
    29161 gggtgagggc catacacaat ttcattgaac ttgagctctc cctgaaaatc ggcccgggag
    29221 cgctggagca gcagagactg ctttcgattc tgctttcgga gttccacctc atcagaggga
    29281 aatgtcgcat tccgggcgac gtcggcgacc agatcgagca gccggctggt attttccgag
    29341 agcgcgttgg cgccgatgat cagagtatcg cggctgctgc tgacgttcag agatcccccg
    29401 atggaagcca gttcttcggc gatctggcgc gaagagcggg tggttgtgcc ttctttcaga
    29461 agggcgccga cgttttcaga taggcccggc agatcggcgg ggtcgaattt tgatcccgcc
    29521 tgaaaatcaa ggcgtacggt gaccagcggg aaacggttgt cctgcaccag caccagcaaa
    29581 agcccgttcg ggagcctggt ctcgaacacc ggtgggagct tgtagacggg cagagggggg
    29641 gtatccggcg gtttggtccg gtcgattttt tgcgcttgca taagagcggc cgaaaacaac
    29701 agaaataagc tgagagggag ccgcgaaatc atggggcctc ctttggcggc gcggcctgcg
    29761 gtttcgggga ggcgggtttc ggcgccggaa ggatctccaa gacagtccgt ttttccggcg
    29821 ccaaatattt cttgaccgcc gcttgcacct gggcgggcgt tacggcgacg aacgcgttga
    29881 tttcctcggt gatccattcg ggcttgccgt caaacatctc gtactgggcc agcagccggg
    29941 cgcggttgag cgagctttgc agctcgttca ggcgggatgc gcgcagaaag gtgcgcgccc
    30001 tctgcatttc tttcgggtcg atcccgtctt tctggatttt gccgatctct tcctggactt
    30061 gatccacgat ctgctttccg gtgaagtttg gcttgcgcag aatgaaaatc ccatacaggc
    30121 cagggtcctt atagtccgaa gcgctgccaa ccggccagcc caggtttgct tcatagcgga
    30181 ccacggactg tttccccttt accaggttca actggaagcg cgaactctcg ccgccggtga
    30241 ggagcacgtc ggccataacc agggcattgt aatcggggga gcgccgcggc ggcccgggat
    30301 agccgataat cacggcggga acctgcgcca gcggatcgtg gtagacgtcc gatcgtgcga
    30361 ccgaatcgga aggctcgcga agatccggag gcgcgggctg cggctgggcc ggaatatccc
    30421 caaaataggt ttccaccagc tttttggcct cggcgatttg gatatctccg acaatcgcga
    30481 gaactgcgtt attcggtgcg tagtggatct tgaaaaactg cgccacgtct tccacggaag
    30541 cggcattcaa atcttcgaac gagccgatga tcgaatgagc gctctgccag ttgcgaaaag
    30601 ccagctccgg ccagcgctcg atgatcgcgg tgttataggg ctggttgtcg aagctgagcc
    30661 gccgttcctg tttgacagcc tctttttggt tggcgagatt ctcggcagta atggccaggc
    30721 tgcgcatgcg gtcagactcc agccacaaag ctacgccgag cttattggaa ggcagcacct
    30781 cgaagtagtc ggtgaagtcg gagtgggtgg agccgttcaa tatgccgccg ttggactcca
    30841 ccagcttaaa atgcatgccc ttgggagcgt tggccgaccc ttcgaacatc atgtgctcga
    30901 acaggtgggc gaaacccgag cggccttttt gctccgaacg cgctccgacg ccgtacatga
    30961 ggtagaccgc gactacggga accgcgttgt cccgcgaaag cacgacgcgc attccgttgc
    31021 gcagtttaaa tttttcgatc ggaatgcgga actcggcggt ctgggccaga gcggccatgg
    31081 cagcgattgc acaaaggaga agaagtttca tactgtcctc attgtacgca gaccgccttc
    31141 gttttgccct cgtttcgacg ggaagccggc cttgccggct tggcccccaa acagcgcttg
    31201 cgtgccgtag actgtccacg gcggccggac ttagggccat ccgcggcgcg atggagccgc
    31261 ccgcgggtct cggccgccgc tggagcgctt cgactggcgc ttcgggcgca tcctgatgcc
    31321 tcctcgggag cgccggtcat gatcaaggcc ggataccggc ttgttagaat acctgaatga
    31381 gccgatccgg cgcgagtctc gccgacggtt ttttccagta ctcgctgctc ctgctggtcg
    31441 cgacaggctt cgccgcagtg ctctcgaccg gcaggctcga cgagccgacc gctgcgctgg
    31501 ccgcggcggc tcttctcggg cgcgccttca tcatcaaggg atggatgaag ccacccctct
    31561 cggccttctg ggtccaggcg ctgaccatcg cctatatcct gttttatcct gtggattttc
    31621 tgtatctttc cggcagtttc ctcggcgcca cggtccgcat gattttcttt ctggcctcta
    31681 tcaagctgct gaccgccaac cacgggcgcg actacctata cctcagcatc attgccctgc
    31741 tcgaggtgct ggccgcggcg atgctaacgt ccagcgccag catcctcgga tttctggccc
    31801 tgtttctggt tttcgcggtc gccgcccgga cgcgatacga gatacagagg aacgcggaag
    31861 tggcagtaca taagcccgcc gccgggggca gcttgggctg gaggctttca gtgttgagct
    31921 tggcgctggc gggtgggatc gcgttgctcg ccctggcttt gttctttgtg gtgcctaggg
    31981 cggcgggagc ctatctgtcg cgcctgccgg cggccgggga atccgtgctg ggattttccg
    32041 acgaaatcgt gctgggcacg cctggccggg tcagaaggga taagacaccg gtaatgaggg
    32101 tcaagattct cgaaggcccc ccgttaaata acttgaagtg gcgcggcgat gcgcttcagt
    32161 atttcgacgg agtcaagtgg tcgaatcctc cctccattgg ccatgtgctt gcaggcgccc
    32221 aaggacgcta tatcgtgggg cggctccggc gaaggagcag gctgctgccg ggcgccatct
    32281 tgcggtatcg cgttctgcgc gcgccgatgg actgtgactc gttattcttg ccggggacgc
    32341 cacagatttt ggaaggcgat tttggacggc ttgaagtcag cgatacggac agcatttcga
    32401 cgcccggcgg ccggtggaag cctctgcgct acgagggctg gagctttatc ggcccggcgc
    32461 ggcccgcgga agccggcgac gtagaaggcc cgtttcaaac gcgcatccgc tcccggtacc
    32521 tgcaattgcc ggcgctggac cccaggattc cggcgttggc ccggcaaata acggaagccg
    32581 aaatgagccc ctattcgcgc gctgttgcca tcgagcgcta tctgcgaact cactttggct
    32641 acaccctgga actgcccctg gaacgcctca gcgacccact ggccgatttc cttttcaacc
    32701 gtaaaaaagg tcactgcgaa tacttcgcgt cggcgatggc ggtgatgctg cggagcatcg
    32761 ggattccggc gcgcgtagcc gacggatttc agagcggcgt atataatccc gtctctggct
    32821 attacacgat tcgcgccagc gaggcgcatg cttgggtgga ggcgtatttt gccggtttgg
    32881 gatgggccac attcgatccc acgccgcccg aaccggcgcc ggaggcgctt ctgggccgct
    32941 cctggcagta tctcgacgcc ctggaaacgt tctggaacga ctggatcatc cagtatgacg
    33001 tcaaccggca ggcgagcctc gctcgaagcg ttcagatcga gtggtacaag gctggctacg
    33061 agaccatgga gcgctgggat cgcgtgtggc tgtgggcggg cggccgctgg gaacgctgga
    33121 aacagaagcc ggctcaggct gtggcgggga ccttgcaggt tctggcgggg ttcctggcag
    33181 cctgtgtagc ggtctgggca ttgccgcgcg tccgcgcctg ggttgtcacg cagcgggtga
    33241 gcctggggcg cggaagtcac aaggactgcg ccttgcttta cgagcgcgct ctcgcgcaaa
    33301 tgcagcgccg cggcttcaat cgggaaacgt ggcagaccgc cggcgagttc gccaagaccg
    33361 tccagccccc ggaatgtccg gctgccgtgt gtgcgctgct gggccaaatt acgcagagtt
    33421 atgaacgagc ccgatttggt tgcgatggcg aagcagggcg gcggctggca ttgctgatca
    33481 ggacgctcga aagatcgccg gtctaacttc gctggcgcat gcagcgcgtg caaactccct
    33541 cgcgccgcat atcgccggcc tcgccgcttt ctcgcattca ccgcctggca gaccagccag
    33601 aaatatgccc ccgtcaggaa tgctaaaata gaggtcggct tcgctccccg actcctcagg
    33661 cagtccggcg ttcgagacag agttgtctgg acgtgcctga gatgctgcga gcaggcctct
    33721 gaatggaggc gcgaggccgt gcggtcactg ccttcccgat gaaaattgga tttgtcagct
    33781 tagg
//
LOCUS       DASREK010000215         4484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2083094, whole genome shotgun sequence.
ACCESSION   DASREK010000215 DASREK010000000
VERSION     DASREK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083094"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="VFA54_11200"
     CDS             complement(<1..860)
                     /locus_tag="VFA54_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZS51421.1"
                     /translation="MAVKFQDYYEALGVPRNASEEEIKRAYRKLARKYHPDVNPGDKD
                     AEERFKQINEAYEVLSDAEKRKKYDALGADWKAGADFTPAGEAGAFRMEFGNLGDVFA
                     GSRFGGFSDFFQTLFGGSPRRGATFSFSQRGPDIESEVALSLEEAHRGGSRTVSVPME
                     DICQICGGSGVKDKRSCPACGGSGSQTRFERFTVNIPKGVREGSVIRVPGKGGSGMGG
                     GERGDLYLRVHLLPHRTFRIRDESDLEVDLPIAPWEAVLGARVTVPTLDGPVEMRIPP
                     HSQGGQMLRLR"
     gene            complement(1047..1499)
                     /gene="trxA"
                     /locus_tag="VFA54_11205"
     CDS             complement(1047..1499)
                     /gene="trxA"
                     /locus_tag="VFA54_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZS51422.1"
                     /translation="MAYSIVRTCTNCRAKNRVPASRLADAGRCGACKAPLGPADEPMT
                     AGDEVSLDGIIHEASVPVLVDFWAEWCGPCRMSGPEVQELAREMAGKALVLKVDIEAH
                     PELAARFGIQAIPYFLVFRDGKLVFQRAGAAPRQEMRQWLELAGPAAA"
     gene            complement(1666..2256)
                     /locus_tag="VFA54_11210"
     CDS             complement(1666..2256)
                     /locus_tag="VFA54_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZS51423.1"
                     /translation="MQRILSGNPAAASRRAKQVRWVVGTLAALVAASWGNVWSQQRGP
                     SERGRQRLEREVRHELVMLPLYGVFDNLAFKVDGYKVDLMGQVTRPTLKADAERAVQS
                     IEGVESVDNKIEVLPLSPNDDRIRLAMYRAIYGDSSLSRYALRAVPTIHIIVKNGNVT
                     LEGAVSSEADKNLAGIRARGVSGVFSVTNNLRVVKE"
     gene            complement(2406..3017)
                     /locus_tag="VFA54_11215"
     CDS             complement(2406..3017)
                     /locus_tag="VFA54_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZS51424.1"
                     /translation="MFGCLLVFPGYSVGQTSRFTGNIGGGFSEPVQGIGAKLNTGWNI
                     TGGAGVNVRPRLSLLGEFMFNDSGINKTALQQINAPNGSTRIWAFTAAPRLQLNHSGR
                     ANFYVIGGGGVYHRTVEFTQPALATVTVFDPWWGVFYPANVLTNQIIGSYGVTKGGLN
                     AGGGLSFALGQTKAKFYAEVRYHHMFTKNVDTTIAPVTFGIRW"
     gene            complement(3133..3564)
                     /locus_tag="VFA54_11220"
     CDS             complement(3133..3564)
                     /locus_tag="VFA54_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZS51425.1"
                     /translation="MICFNATMKELTMRAQDLEKFRVMLEAKRNELSRLLQQRDEITI
                     QRAAEPLEESQLAAQRDMAVWSRHLESRLLREVRAALERISDGTYGFCLHCEGEISPK
                     RLAAVPWASYCIRCQEELDNQQAQQAAGPDLRYLAALAQAA"
     gene            3771..>4484
                     /locus_tag="VFA54_11225"
     CDS             3771..>4484
                     /locus_tag="VFA54_11225"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HZS51426.1"
                     /translation="MKSLLAVVRPALLCLPGFLVSSGVTWPQPVPLRPDQLDQLVSRI
                     ALYPDPLLAQVLTASTYPDQIPEAAAWADQHSYLRGDALARAISDDHLPWDPSVLALL
                     PFPSILDMMSRDMAWTRQLGDAVLVQRAEVMDAVQRMRRKAYDYGYLRSNLYIRVIAM
                     PGYIEVLPAVPAYIYVPVYDPLIVFAPPRRGISVSVAITFGPPVAVEPAFVAFGWRQA
                     GFLWPSHVVVVDRRPWQRTW"
BASE COUNT          875 a         1401 c         1221 g          987 t
ORIGIN      
        1 cggagacgca gcatctgccc gccctggctg tgaggcggaa tccgcatctc gaccggaccg
       61 tcgagggtcg ggactgtcac tctggcccct aaaaccgcct cccagggagc gatgggcaga
      121 tccacttcca gatcggattc atctcgtatc cggaatgtcc ggtgaggcag caaatggacg
      181 cgcaaatata ggtcgccgcg ttcgccgccg cccatcccag agcctccctt tcccgggaca
      241 cgaatcacag aaccttcccg cactccctta ggaatattga ccgtgaaccg ctcgaagcgg
      301 gtttgggagc cggaaccgcc gcaagcgggg cacgacctct tgtctttgac gcctgagccg
      361 ccgcagatct gacagatgtc ttccatcgga acggaaaccg tccggctgcc gcctcgatgc
      421 gcctcctcca ggctgagagc aacctcagac tctatgtccg gcccgcgctg ggaaaaagaa
      481 aaggtagcgc cgcgccgtgg tgatccgccg aacagcgtct ggaagaagtc gctgaatcca
      541 ccaaatcggc ttccggcgaa aacgtcgccc aggttcccga actccatccg aaaagctccc
      601 gcctcgcccg ccggcgtgaa gtcggccccg gctttccaat ccgctcccaa agcatcatac
      661 tttttgcgtt tctcggcatc cgagagaact tcgtatgctt cgttgatctg cttgaagcgc
      721 tcctcagcat ccttgtcgcc gggattcaca tcgggatgat acttacgcgc caacttgcgg
      781 tacgcgcgct tgatctcctc ttcgctcgcg tttctgggca ccccgagcgc ctcgtaatag
      841 tcctggaact tgactgccat gtgtgctgca ccggaagagc aaagccctgc ccgcgccctg
      901 cattcacgac cgggctgcga atccgagcat ttcccgccat agccgcatcg aaaatccggt
      961 ggagagggcc ccgtccagta ccgagtctct gatcttcgcg ccccgctgcg gagtttttct
     1021 ggacgcggat tccgaagtct ctcatctcag gctgcggccg gtcctgcaag ctccagccat
     1081 tggcgcatct cttgccgcgg agcggcgcca gcgcgctgaa acaccaactt tccgtcccga
     1141 aagaccaaaa aatatgggat cgcctggata ccgaaccggg ccgcgagctc tgggtgggcc
     1201 tcgatatcca ccttgagcac cagcgctttc cctgccattt cccgcgcgag ctcctgcacc
     1261 tccggaccgg acatgcggca aggcccgcac cattccgccc aaaagtccac cagcacagga
     1321 acagaggcct cgtggatgat gccgtcaagc gaaacctcgt cgccggccgt catcggctcg
     1381 tcggcgggcc cgagcggcgc cttgcaggcg ccgcagcgcc ccgcgtcggc cagccggctg
     1441 gcaggaacgc gattcttggc cctacagttc gtacacgtgc ggacgatcga gtaggccatc
     1501 ggacgcctca attcctccaa aattagcggt acgccggcct gcgccgtttc aggatcaatt
     1561 ctcgactcct ccagcggatc ctccgttttc gtttgagcac ggctcgcaaa agcctctctc
     1621 gcccgatcca gaggacgaaa ccagagcgga cggcgtcaaa acccctcatt cttttaccac
     1681 gcggaggttg ttagtcactg agaacacacc tgatactcct ctcgcccgaa tgccggctag
     1741 attcttgtcg gcttcgctgg atacggcgcc ttccagggtg acatttccat tctttacgat
     1801 gatgtgaata gtagggacgg ccctcagggc gtagcggctg aggctggaat ctccataaat
     1861 cgcccgatac attgcgagcc tgatgcggtc gtcgttcggt gagagcggca gcacttcgat
     1921 tttgttgtcc acgctctcca caccttcaat gctctgcaca gccctttccg cgtcggcctt
     1981 gagagtcggc cgggtgacct ggcccatcag gtccaccttg tagccatcga ccttgaacgc
     2041 aaggttgtca aacacgccat agagcggaag catcaccagt tcgtggcgca cctccctctc
     2101 tagtctttgc cgcccgcgct cggagggccc ccgctgctgg ctccaaacgt ttccccagct
     2161 cgcggcaacc agagcggcta gcgttcccac aacccagcgg acttgtttcg cgcggcgcga
     2221 ggcagcagcg gggttccccg acagtatcct ttgcatgtgc gcatcctcct ttgtcctctc
     2281 aaacaatcga atattaccct tggctctccg agtggtagcg gccgcttggg cattcaaagg
     2341 ctcagaaagc gcgacgggcg gtccgtaccc gccgcgcatt gcggttttgc gcgcaggcgt
     2401 cctccctacc agcggatgcc aaacgtgact ggcgctatcg tcgtgtcgac gtttttcgtg
     2461 aacatgtggt gataccggac ttccgcgtaa aatttcgctt tggtctggcc gagcgcaaag
     2521 ctcaggccgc cgcctgcatt cagccctcct tttgtcacgc cataggaccc tatgatctgg
     2581 ttggtcaata cattcgcagg gtagaaaacg ccccaccacg gatcgaagac tgtcaccgta
     2641 gccagcgccg gctgggtaaa ttcgacagtg cggtgataaa ccccaccgcc gccaatcacg
     2701 tagaagttcg ctcggccgct gtggttcaac tgcaaacgag gagcggctgt gaaagcccat
     2761 attcgcgtgg agccgttcgg cgcattgatt tgctggagcg ccgttttgtt gatccccgag
     2821 tcgttgaaca tgaactcgcc caagaggctg agccggggcc tgacgttcac ccccgcgccc
     2881 ccggtgatgt tccagcccgt gttgagcttg gcccctatgc cctgaaccgg ttcagaaaag
     2941 cctcccccga tattacccgt gaatcgagaa gtttgaccca cggaatatcc gggaaaaacg
     3001 agtaagcatc cgaacatcaa cagacaccat ttgcgcgtca cggatatgcc tcctttcgtc
     3061 aaacttcctt cttcaaattg aggttgcgct cgattggcag gtctgtcggc tggcaacgcc
     3121 gccggtctct gctcaagcgg cctgtgccaa ggcggctaag taccgcaagt ccggtcctgc
     3181 cgcttgttgc gcctgctggt tgtccagctc ttcctgacag cgaatgcaat acgacgccca
     3241 aggcacggcg gccagccgct tcgggctgat ctcgccctca caatgcaggc aaaagccgta
     3301 agtgccgtca gaaatgcgtt caagcgcggc ccggacttcc cgaagcaggc gcgattccaa
     3361 atgacggctc caaaccgcca tgtcacgctg cgctgcaagc tggctttctt ccaaaggttc
     3421 ggctgctcgc tggatcgtaa tctcgtctcg ttgctggagc agcctcgaca gttcattgcg
     3481 cttggcttct aacatcaccc tgaatttttc gaggtcttga gcgcgcatcg tcagctcctt
     3541 cattgtggcg ttaaagcaaa tcatcaagtt cttaatatat aatcttagcg tatctatgtc
     3601 aatatatttt agtatgaact taacgaaaaa attttaagtg atttattttc aataccttaa
     3661 tatctaaaaa gatgtcctag ttagctgtaa cttacgggtc ttgaggcagc gtcagaaaaa
     3721 aacggaagag aaggcacggt agcattgctt ttcgtaagga ggataaaaag atgaagagtc
     3781 tgttagctgt cgttcggccg gccctgttgt gcctgccagg attcttggtc agttcggggg
     3841 tgacctggcc ccagcccgtc cccttgcgtc cggatcagct cgaccaattg gtctcccgca
     3901 ttgccctgta cccagatccc ttgttagcgc aggtcttgac ggcttccaca tatccggatc
     3961 aaatcccaga ggcggcggcc tgggcagacc agcacagtta tcttagaggc gatgctttgg
     4021 cccgggcgat ttctgacgat catttgccat gggatccctc tgttttggcg ctattgccgt
     4081 ttccttcaat cctcgacatg atgagccgcg atatggcctg gacgcggcaa ttgggcgatg
     4141 cggtcctcgt ccaacgcgcg gaggtgatgg atgccgtcca gcggatgcgc cgaaaggcat
     4201 atgattacgg gtatttacga agtaatttgt atatccgagt gatcgcaatg cccgggtata
     4261 ttgaggtcct ccccgctgtg ccggcctaca tctatgtccc cgtttatgat cctctgatcg
     4321 ttttcgcacc gccgaggagg ggaatttcgg tgagcgtcgc catcactttc ggtccgcctg
     4381 ttgcggttga gccggcattt gtagcgttcg ggtggaggca agccggtttt ctatggccct
     4441 ctcacgttgt cgtggtggac cggcggccct ggcagcgcac gtgg
//
LOCUS       DASREK010000216         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1425580, whole genome shotgun sequence.
ACCESSION   DASREK010000216 DASREK010000000
VERSION     DASREK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425580"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..571)
                     /locus_tag="VFA54_11230"
     CDS             complement(152..571)
                     /locus_tag="VFA54_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS51427.1"
                     /translation="MRFQTWTAMGTALLFCFFAGAAAAQNNAAERGKYLVENVGVCQT
                     CHTPKLENGEFDKSKWLKGATLNIQPLGTIPKWHKTAPDLTSGSDLFKRWGPDGLVRF
                     LETGKNPRGNPADPPMPEYHMSHADAVAVVEYLKTLP"
     gene            802..1719
                     /locus_tag="VFA54_11235"
     CDS             802..1719
                     /locus_tag="VFA54_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS51428.1"
                     /translation="MDFDQLHTFLEIVRLKSFSRAAQTCFRTQPAISAQIRQMEQELG
                     TPLFERFGSRISLTTAGKIFAEYAQQLLEVRRRAIEAVAELDHIPKGELVIAANEATC
                     IHVLPEVFSCFKKRFAEVQLQVTRANGSRAVQAVLENSVDFAITQLPIQERKLEVVKI
                     YSDEIRLLVPPAHPLARAKSVTGRQAAEYPLLLPKTGRTRTLIDQFLEPAETGVHISM
                     ELESSEMIKRFVTAGMGIGFMAVTSAKEEIEAKLLKDVPLGPPPLFRTLGLIYRRDKP
                     LSRAALGFIEVVLEFARKAAGDENIEAAG"
     gene            1737..2171
                     /locus_tag="VFA54_11240"
     CDS             1737..2171
                     /locus_tag="VFA54_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51429.1"
                     /translation="MTCPNCGRQIWMMDAARRRCIHCGRLFDRTALGLVRTSAVRIAT
                     GESDVVYPSVDEVPPELRRGLQRAIVNPNSETIVIADEQGRQEIFQVINGLNPDAQKK
                     VLAAVKLAQPPAPPLRALRTWRLFFAVSGLLLLLLFLWWVWK"
     gene            2269..>3405
                     /locus_tag="VFA54_11245"
     CDS             2269..>3405
                     /locus_tag="VFA54_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HZS51430.1"
                     /translation="MKSVIRIANGQGFWGDWLEAPVRLVEQGPIDYLSLDYLAEVTMS
                     ILQKQKRINPKLGYARDFVPLMARILPKREHLRVIANAGGVNPQGCAEAVLAAARSAG
                     VSGLRIAVVLGDDVYDRLDDMLARGADLRDIDSGRPLREIRDRVVSANAYLGAFPLVE
                     ALRTGAHVVIAGRCTDTALALAPMIYEFDWNEQDWDRLAAGTVAGHIIECGAQTTGGN
                     SQVDWQSIPDMANIGYPIIEARPDASFCVTKHAGTGGRVSSAVVKEQLLYELGDPHRY
                     ITPDCVADFTSIQLRDAGPDRVEVCGIRGSPRTPFLKVSISYSAGWKAIGTMVYSWPQ
                     ALQKAQAADRIVRQRLAALGLRFDEIRTGFLGVNACHGPIAPPNP"
BASE COUNT          640 a         1034 c         1041 g          690 t
ORIGIN      
        1 cttcttcgat ccctctgccc attggcatag cgcccgcagg cagcgaagtc tgcgggatcc
       61 tgagtctcgc aggttacgct caaccggcgc cgcccacacc atggcgcggg tttagacccc
      121 tctaccagcc tctgccggca accttgcggt gttatggcag cgttttcaag tactccacaa
      181 ccgcgaccgc atcggcgtga ctcatgtggt actcaggcat aggcgggtca gcggggttcc
      241 cgcgagggtt tttgccggtc tccaggaacc ttaccaagcc gtccgggccc cagcgcttga
      301 aaagatcgct gccagaggtg agatcaggcg cggtcttatg ccatttggga atagtgccca
      361 gaggctggat gttcaacgtg gcgcccttga gccacttcga cttatcgaac tctccgttct
      421 ccagcttcgg ggtatggcac gtttggcaga ccccgacgtt ttccaccaaa tatttgcccc
      481 gctcggccgc gttgttctgg gccgccgcag ccccggcaaa aaaacaaaac agcagggctg
      541 ttcccatggc tgtccacgtc tgaaagcgca ttgcaagctc ctttgtggga tgatatacgc
      601 ctggaaggtg cgccggcaag aggcccagac ggccccggtg gcagggaggt ccggtggttt
      661 gcaggacttc gttgcggcct cgcccgatga atccaaatca tgttcatcaa ggttattgat
      721 gaattcgggg cgccggcgca ggtgtacgct gttactgtgg gtttttctac ccgaaagccc
      781 agcctgtgat ccggcccaag catggatttc gaccagcttc acacttttct tgagattgtc
      841 cggctgaaga gcttttcgcg ggcggcgcag acctgttttc gcacgcagcc ggccatcagc
      901 gctcaaatcc gccagatgga gcaagaactc ggcacacccc tgttcgagcg cttcggaagc
      961 cggatctcgc tgacaacagc gggcaagata ttcgccgaat atgcccagca gttgctggag
     1021 gtgcgccgcc gggccataga ggccgtcgcg gagctggatc atattccgaa gggcgagttg
     1081 gttatcgctg ccaacgaagc cacctgcatc cacgtgctgc ccgaggtgtt ctcctgtttc
     1141 aaaaagcgat tcgccgaagt gcagctccag gtcacgcgag ccaacggctc ccgcgctgtg
     1201 caggctgtgc tcgaaaattc cgtggatttt gccatcacac aactgccgat ccaggaacgc
     1261 aagctcgagg tagtgaagat ttacagcgac gaaatccggc tgctggttcc gccggcccac
     1321 cccctggcgc gcgccaaatc ggtgaccggc cgtcaggcgg ccgagtaccc gctgctgctg
     1381 ccaaagaccg gacgcacgcg aactttgatc gaccagtttc tggagcccgc cgaaacgggc
     1441 gtgcacatct cgatggaact ggagtcgagt gaaatgatca agcggtttgt gaccgccggc
     1501 atggggatcg gattcatggc tgtaaccagc gcgaaagagg aaatcgaagc gaagctgctg
     1561 aaagacgttc ctctgggccc tccgcctctg tttcgtacgc tcggtttgat ctaccggcgt
     1621 gacaaaccgt tatcgcgggc cgccctgggt tttatcgaag tggtgctgga gtttgccaga
     1681 aaggccgcgg gtgatgaaaa catcgaggcc gcgggttgac gcaaacatag accgaaatga
     1741 cctgtcccaa ctgcggccgc caaatctgga tgatggacgc agcccggagg cgctgcatcc
     1801 attgcggccg gctttttgac cgcacggccc tcggcttggt cagaacctcg gccgtccgga
     1861 tcgcgacagg tgaaagcgac gttgtgtatc catccgtaga tgaggtgccg ccggagttgc
     1921 ggcgcgggct ccagcgggcc atcgtcaacc ccaatagcga aacgattgtg atcgccgacg
     1981 agcaggggcg ccaggaaatt ttccaagtca ttaatggcct caatcctgat gcgcagaaga
     2041 aagtcctcgc ggcagtgaag ctcgcccagc cgcccgcccc gccgctgcgg gcgcttcgga
     2101 cgtggcgcct atttttcgct gtttcgggct tgctcctgct tctgctgttt ctatggtggg
     2161 tttggaaata agacatttgg gctcgctggt gcgcagaaga aggcgcggag gcaaagcaag
     2221 attctcttcg gctccgcgtg tgattctgcg ccgctcacgt cgtctctcat gaaatccgtc
     2281 atccgcatcg ctaacggaca gggcttctgg ggcgactggc tcgaagcgcc ggttcgcctg
     2341 gtcgagcagg ggccaatcga ttacctcagc ctggactacc tggctgaggt taccatgtcg
     2401 attctccaga aacagaaacg gatcaacccg aagctcgggt acgctcgcga ttttgtgccc
     2461 ctgatggcca ggatcttgcc gaaacgggag catctgcggg tgatcgccaa cgcgggcgga
     2521 gtgaatcccc agggatgcgc cgaggctgtt ctcgcggcgg cccgcagcgc cggagtttcc
     2581 ggcctcagga tagcggtcgt gctgggtgac gatgtctatg atcgcttgga tgacatgctg
     2641 gcccgcggcg ccgatctgcg cgacatcgat tccggccggc cactgcggga aattcgcgac
     2701 cgcgtggtaa gcgccaatgc ttatctcggc gctttcccgc tcgtcgaggc gctgcgcacc
     2761 ggcgctcatg tggtcattgc aggccgctgc accgataccg ccctggctct ggcgcccatg
     2821 atttacgagt tcgattggaa cgagcaggac tgggaccgct tggccgccgg tacggtcgcc
     2881 gggcacatta ttgaatgtgg cgctcagaca accggcggca actcccaggt agattggcag
     2941 tcgattccgg acatggcgaa tatcggctac ccgattatcg aggcccgccc cgacgccagc
     3001 ttctgcgtaa ccaaacatgc gggcacgggc gggcgtgttt catcggctgt ggtcaaggaa
     3061 cagttgcttt acgagctggg cgatccgcac cggtacatca ctcccgattg cgttgccgat
     3121 ttcacctcga ttcagttgcg cgacgcgggt cccgaccggg tcgaagtctg cgggatccgc
     3181 ggctcgccgc gcactccgtt tctgaaagtc tccattagct attccgccgg ctggaaggca
     3241 atcggaacga tggtgtatag ctggccgcaa gctctacaaa aggcgcaggc cgccgaccgc
     3301 attgtccggc agcgcctggc ggcgctcggt ctccgcttcg acgaaatccg cacgggattt
     3361 ctcggggtga acgcctgcca tggcccgata gcgccgccca acccc
//
LOCUS       DASREK010000217         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1583436, whole genome shotgun sequence.
ACCESSION   DASREK010000217 DASREK010000000
VERSION     DASREK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583436"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..>2408
                     /locus_tag="VFA54_11250"
     CDS             21..>2408
                     /locus_tag="VFA54_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVC-type heme-binding CxxCH protein"
                     /protein_id="HZS51431.1"
                     /translation="MPRLRNVAAAAVWLLLESGCSHSRPPYDPQEALKTFRIEKGFRI
                     ETFLSEPDIRSPVAMEFDENGRIYVVEDPGYPLDTEHKLGRVILLEDANGDGRPDRRT
                     VFVDKLVMPTGVMRWKKGILVTDAPNVWYFEDADGDGKADVKRIVLTGFAFTNPQHTV
                     NNPVYGLDNWIYLAHEGPATAVIFQDQFGDPGGDLRFPDRSGGAVLKPQRRGVRFRPD
                     TYQLEYISGNTQFGQSFDQWGRHFLHNNSNHIREEVIAARYLQRNPDLLIGSAMQDIS
                     DHGAAAVVYPITKNPRFELLTNVGEFTSACGLTVYLGGLWPQLGLFSLVAEPAHDLVH
                     RDVLSPSGVTFCARRAQQGSEFLASTDSWFRPVNFTIGPDGAIYVLDYYRPIIEHPEW
                     MSSEHHHDNKQLYEGQDRGRIYRIVPDTQPPLPLPKGIHLDQAPDEELVRHLADPNIW
                     YRRTAQRLLVDRQSPGSVAPLIRLAETSASPVARVHALWTLDGLGKLDASLIARALAD
                     SEPGVRENAIELAESRLSSSPELAGKLLKMRDDPDLRVQFQLLCTLGNIDSAASREAQ
                     NELLRRRIEDPWMQVAALSAKSDRAPQLFRMAISGRLIRAKSEGGEAFFRQVSAVIGA
                     RAKAGEIHSVLQNLLARGGPDSEWWRAAVLEGLARGLRGKNLQENSSRELLLRLFESS
                     REPVRRASLDLIRVAGLPPGRAAGAAMDRAVKIAQDKTADASLRADSIRFLGLAGAGP
                     PAAGREELFKKLVDPAEPERVQAAAVEALGRMPGEEIGAFLIERYRSLTPAVRSEA"
BASE COUNT          453 a          747 c          749 g          459 t
ORIGIN      
        1 gacacaagga gttcggacaa atgcccagat tgcgtaatgt cgctgcggct gcggtctggc
       61 tgcttctgga atcaggctgc tcgcactccc gtccgccgta cgacccgcag gaagctctga
      121 agacttttcg cattgaaaaa ggattccgca tcgaaacttt cctcagcgaa ccggatattc
      181 gcagcccagt cgccatggag ttcgatgaaa acggccggat ttacgtcgta gaggaccccg
      241 gctatccgct cgatacggaa cacaagctgg gccgcgtcat cctgctggag gatgccaatg
      301 gcgacggccg ccccgaccgc cggaccgtct tcgtggacaa gctcgttatg cccacaggcg
      361 tcatgcgctg gaaaaaaggc atcctggtga ccgatgctcc gaatgtctgg tatttcgagg
      421 acgccgacgg agacggtaaa gcggatgtca agcggatcgt gctcactggt tttgccttca
      481 ctaacccgca gcacacggtc aacaatcctg tctacggtct cgataattgg atttatctcg
      541 cgcacgaggg tccggcgacg gcggtaattt ttcaggatca gttcggagat cccggcggcg
      601 acctgcgctt cccggaccgc agcggcgggg ccgtcctcaa accgcagcgc cgcggcgtgc
      661 gcttccggcc cgacacgtat cagcttgaat acatctcggg caacacgcag ttcggccaga
      721 gcttcgacca gtggggccgc catttcctgc acaacaactc gaaccacatc cgggaagagg
      781 tgatcgcagc ccgctatctc cagcgcaatc cggacttgtt gatcggttcc gccatgcagg
      841 atatctccga tcacggcgcg gccgctgtcg tttaccctat tacgaaaaac ccccggttcg
      901 agctgctcac caacgtggga gaattcactt cggcttgcgg gctgaccgtt tatctcggcg
      961 ggttgtggcc gcaactcggc ttgttctccc tggtcgcaga gccagcgcac gatctggtgc
     1021 accgggatgt tctgtcgcct tcgggcgtaa cgttctgcgc ccggcgcgca cagcagggct
     1081 ccgagtttct cgcctccacc gatagctggt tccggccggt caatttcacg atcgggccgg
     1141 atggcgccat ttatgtgctc gattactacc gcccgatcat tgagcatccg gagtggatgt
     1201 ccagcgaaca ccaccatgac aacaagcagt tgtatgaggg ccaggaccgc ggccgcatct
     1261 accggatcgt gcccgatacg cagcccccgc tgccgctgcc gaaaggaatc catttggatc
     1321 aagccccgga tgaggaactg gtgcggcatc tcgccgatcc caacatctgg tatcgccgca
     1381 ccgcgcagcg cctgctggtg gatcgtcaaa gccccggctc cgtcgcgccc ttgatccgcc
     1441 tggcggagac cagcgcttcg ccggtagctc gggtgcatgc cctgtggact ctcgacggct
     1501 tgggaaaact ggatgccagc ttgatcgcac gagccctcgc ggacagcgaa cccggcgttc
     1561 gggagaacgc catcgaactg gccgagtccc gcctctcgag ctctcccgag ttagccggga
     1621 agctgttgaa gatgagagat gatcccgatt tgcgcgtgca gtttcagctc ctgtgcacgc
     1681 tgggaaacat cgattcggct gcctcccgcg aagcgcaaaa cgagctgctg cgccgccgca
     1741 tcgaagatcc gtggatgcag gttgccgcgt tgagcgccaa gtcggaccgt gcgccccaac
     1801 tcttccggat ggcgatttcc ggccgactga tccgggcgaa atccgaaggc ggcgaggctt
     1861 ttttccggca agtttctgct gtgatcggag cgcgcgccaa ggcgggcgaa atccacagcg
     1921 ttctgcaaaa tctgctcgcc cgaggcggcc ccgattcgga gtggtggcgg gcggcggtgc
     1981 tcgaaggact ggcgcggggc ctccggggaa agaatttgca ggagaattcc agccgcgaat
     2041 tgctgctgcg gctgttcgaa agctccagag agcctgtgcg gcgagcttcg ctcgacttga
     2101 tccgcgtcgc tggcctgccg ccgggacgcg cggccggggc tgctatggat cgcgccgtaa
     2161 agattgcaca ggataaaacc gcggatgcaa gcctgcgggc ggactccatc cgctttcttg
     2221 gcttagccgg tgcgggtccg ccggcggcag ggcgcgagga actcttcaaa aaactggttg
     2281 atcctgccga gccggagcgc gtgcaagccg cggcagtgga agctctgggc agaatgccgg
     2341 gcgaagagat tggagcgttt ctgattgagc gataccgcag cctgactccg gccgtccgga
     2401 gcgaagcg
//
LOCUS       DASREK010000218         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2412210, whole genome shotgun sequence.
ACCESSION   DASREK010000218 DASREK010000000
VERSION     DASREK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412210"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1269)
                     /locus_tag="VFA54_11255"
     CDS             complement(337..1269)
                     /locus_tag="VFA54_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HZS51432.1"
                     /translation="MTQAALAALDQLSFWPSLPRNEWEDTYQTLHMWTQIAGKIRLEL
                     GPKLNHWWGATLYVSARGLTTSPIPYRGRMFEIQFDFLDHQLEILASDGGRRSLPLEP
                     QPVAAFYDKLMGALRSLEIEVTINTKPQEVADPIPFEQDYRHASYDREYASRFWRILL
                     SADMVLNEFRSRFIGKCSPVHFFWGSFDLACTRFSGRPAPPRKGVITSEAYSHEVISA
                     GFWPGGGGLEAAFYSYTAPSPPGLANEPIQPPAAFWSKQLSEFILMYEDVRRAKSPSS
                     TLLEFCESTYQAGARLANWDRAALERDASVGRTA"
     gene            complement(1400..>2625)
                     /locus_tag="VFA54_11260"
     CDS             complement(1400..>2625)
                     /locus_tag="VFA54_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS51433.1"
                     /translation="GEVDVDLWNEHLARYAFAARRAMGRRVLDAGCGIGYGSAQLAQI
                     ARHVVGLEIAAGVARSARQRYKAPRLDFVSGDCRKLPFPDEAFDLVVAFEVIEHLEGW
                     ESLLDESRRVLSAPGELIISTPNRLYYAESREAPNPFHVHEFEYEEFCAALSARFAHV
                     RIFLENHSEGVIFSLPETTEIESFVESDAGSGPVDPHSSHFFVAVCSSQPIYSSPPFI
                     YIPQSGNVLREREKHIALLQSEIEQKDRWLAEVKESLRNLHNRHQALEAEAAENQQRA
                     QQAIDALEQENARKAAWAEQLNAEILRLGEIIRTLQSDIEEKTQWALNLEAERVRLVE
                     EVASVRRDLKTCVDQLHATEAELENRTAWAQSLDQQVARLTADLNAWLGSPAYRIGKR
                     LRLAPIPSSDPRRRD"
BASE COUNT          503 a          790 c          830 g          502 t
ORIGIN      
        1 gtggagatcg gccgcgacta cggcgcccag atcgaaatta ccagcgggct ggcggagggc
       61 gcttccgtgg tcgtgaaccc gaccgacgat ttgcaggaag gatctcaagt cgagccggtc
      121 gcccgggcga agccggggaa atgatatcca ttctgagctg ccccggacgc ggctctttgc
      181 gggagtttcg gcgcccgtca ggcatcgccg gctgcggagc cgccctgccg gttcttgcgg
      241 agaggcggcg ccttccgggg tgtccaaccg gatccccggc attcgataaa tgcgccacat
      301 acaccaaacc aaatccctgc tcggttctgc gatagattag gcagtgcggc ccacgctggc
      361 atcgcgctcg agagcagctc ggtcccaatt ggcgagccgc gccccggctt ggtacgtact
      421 ttcgcaaaac tcgagtagag tagagctcgg tgatttggcg cggcgcacgt cctcgtacat
      481 caggataaat tcggatagct gtttgctcca gaaagctgcc ggcggctgga tcggctcatt
      541 ggcgagaccg ggaggcgaag gcgctgtgta ggaataaaac gccgcttcca agccgccgcc
      601 tccgggccag aatccggcgc tgattacctc gtgcgagtag gcttcgctgg tgataacgcc
      661 tttgcgggga ggagccgggc gcccggagaa gcgggtgcac gccagatcga agcttcccca
      721 gaaaaaatgg acagggctgc atttgccgat gaagcgggag cgaaactcgt tgaggaccat
      781 gtccgccgaa agcaggatgc gccagaaacg gcttgcgtat tcccggtcgt aggaggcgtg
      841 ccggtagtcc tgttcgaagg gaatgggatc ggcgacttcc tgcggcttgg tattgatagt
      901 aacttcgatc tccagagagc gcaaggcgcc catgagcttg tcgtagaagg cagccaccgg
      961 ctgcggctcg agcggtaaag accggcgccc gccgtcgctc gccaggatct cgagttggtg
     1021 atccaaaaaa tcgaactgaa tttcaaacat gcggccgcgg taagggatgg gcgaagtcgt
     1081 caggccgcgc gcgctgacgt acagggtcgc tccccaccaa tggttcagct tcgggcccag
     1141 ttccaatcgg atcttgccgg cgatctgggt ccacatatgc agggtctgat acgtatcctc
     1201 ccattcgttc cgcggaaggc tgggccagaa cgaaagctga tccagcgcgg ccaaagcggc
     1261 ttgagtcatg gtttcgctcc tgaatgcacc gccgggcgct gcccgctcgg ggcatggtca
     1321 cagtctggca caggccagcc gggggcgcat agcggttggg aaagcggtcg aagcgattgg
     1381 tttgtgagcc tccagaaagc taatcccggc gccgaggatc ggagcttggt atcggagcga
     1441 gccgcaggcg ctttccgatc cggtaggccg gagaacccag ccaggcattc aggtcagcgg
     1501 tgagccgggc cacctgctga tcgagactct gcgcccaggc ggtgcgattt tcgagctcgg
     1561 cctcggtggc gtgcaactgg tcgacgcatg ttttcaaatc gcggcgcacg ctggccacct
     1621 cttcgaccag ccgcacgcgt tcggcttcga gattcagagc ccactgcgtc ttttcctcga
     1681 tatcagattg cagcgttcga ataatttcgc ccagccgcag aatctccgcg ttcagttgct
     1741 ccgcccaggc ggctttgcgg gcgttttcct gctcgagcgc gtcaatcgct tgctgcgccc
     1801 gctgctggtt ctcggcggct tcggcttcca aggcctgatg ccggttgtgg aggttgcgaa
     1861 ggctctcctt cacttcggca agccagcggt ctttctgctc gatctcgctc tggagcagcg
     1921 caatatgctt ttccctttct cgcagcacat tgcccgactg cgggatataa atgaacggcg
     1981 gcgagctata aatcggctgg ctggaacata cggcgacgaa aaagtgcgac gagtggggat
     2041 ctacaggacc cgatccggcg tccgactcca caaagctttc gatttccgtc gtttccggca
     2101 gggagaaaat gacgccttcc gagtgattct ccaggaaaat gcgtacgtgc gcgaagcggg
     2161 ccgataaggc ggcgcaaaac tcctcatatt caaattcgtg gacatgaaac ggattcggtg
     2221 cttcccggct ttccgcgtag tagaggcgat ttggcgtcga gatgatcagt tcccccggag
     2281 cggaaaggac gcgccgcgac tcgtcgagca gactctccca tccctcgaga tgctcgatca
     2341 cctcaaatgc gaccaccaga tcgaatgctt catcgggaaa gggcagcttg cggcaatcgc
     2401 cgctcacgaa atcgagccgc ggagccttat agcgctggcg cgcggatcgc gccaccccgg
     2461 cggcgatttc cagtcccact acatgccgcg cgatttgcgc caactgggcc gagccgtagc
     2521 cgatgccgca gcccgcgtcg aggacgcggc ggcccatggc ccgtctggcg gcaaatgcat
     2581 agcgggccag atgctcgttc cacagatcca cgtccacttc gcccg
//
LOCUS       DASREK010000219        29190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_600998, whole genome shotgun sequence.
ACCESSION   DASREK010000219 DASREK010000000
VERSION     DASREK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 29190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29190
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600998"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VFA54_11265"
     CDS             <1..1306
                     /locus_tag="VFA54_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS51434.1"
                     /translation="GPLEMTAGWPLATLFTLAVAASMAELCSAFPTSGAMYHWAAHLG
                     GPIWGPACGWFVAWLNILGLAAALAGINYSCAQFVLPFLGIASTYSNLLAAFGLILVT
                     QALLNHYGIRLVAILNDCSVTVHIAGVVALVAALLLLARLQPARFLFQATTTNGRFPY
                     GWAFVLGLLQAQWTYTGFDGSAHVAEETSDPRRRAPWGIVLSVAVSGIVGYLLILSLT
                     LAIRAIPSVLETRDAQGNPLPAAIVILQQALGARAGNAMSALASMAMWFCGLSCVTSA
                     SRAIFSLARDQGAPFSALLRRVSPRHGTPAAAIWVFTGASFAAMLWTGAIPVVTSLST
                     VALYAAYIVPIMLALRSRSSGWQHSAPWSLGRWGARVNLVAIVYTLFICAVLVMPPNV
                     LAGKTFLGLMAGLILLYALVVRRRYRGPQWSRGEAIARGGAV"
     gene            1413..2756
                     /locus_tag="VFA54_11270"
     CDS             1413..2756
                     /locus_tag="VFA54_11270"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZS51435.1"
                     /translation="MNPVLVKRKFDERGTKRVKLALVDIDGVLRGKYVSLDKFWSAVE
                     SDLGFCDVIFGWDISDALYDNVRFTGWHTGYPDMRARIDTTTFRLIPWEADTAFFLLD
                     LIDKKGAPIAVSPRQVFRQVVERAASLGYTPYFSAEYEFFFFRETPQSLREKNYRNLS
                     SLSPGMFGYSVLRAAANSEFVLQVIDQLAEFDVQLEGFHTETGPGVYEAAVAAAPGLQ
                     GADRAALFKTAVKEIAARHGLIPTFMAKWNADLPGSGGHIHQSLWDSKKGQNLFAGEN
                     CGMSQLMQHYIAGLAASLPELTAMMCPTINSYKRMVPGFWAPVNATWGLDNRTSAVRA
                     IVGEDKSTRVETRLSGADMNPYLAMAASLAAGLDGIERKLELPPPAKDAYAANALPLP
                     ANLAEATRLFRQSSLARKAFGEEFVEHYAATREWEVRQYERAVTDWELARYFEAV"
     gene            3338..4003
                     /locus_tag="VFA54_11275"
     CDS             3338..4003
                     /locus_tag="VFA54_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51436.1"
                     /translation="MSAAGLYYAATRVKSRLLSAAGVARATEHGRSSAGSKIDACALL
                     SKEELGELLGIHVDRAQPIADGGEPGCAFFASPETLRQLARDSLSKAPEQVKTAEAKG
                     RVKGDNPLANVNTSTIQGLEGIVKTIAAAGDASQGGGKVFSFTVDPKFDAESWPLFLK
                     TMSVVPGFEPLAGVGDHALMGPFGQILYVQKGNVKVTLELTSVPDAHGKGVDLARKII
                     SRL"
     gene            4366..5196
                     /locus_tag="VFA54_11280"
     CDS             4366..5196
                     /locus_tag="VFA54_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZS51437.1"
                     /translation="MKAAVVLIALSVLAIPFPAATQTVEPGQTPAADAPTSAPLVTQE
                     PSPVSPAADRPVSWKGLPLNLLSDQKRIWSFPARLARGQSWIPATAVVGTTTGLFFVD
                     PTEAAYFHRTPAFHGWNNIFTSNATLAGTIVVPASLYAAGLVRKDPKMQHTALLAGEA
                     VADAEIVTTVLKDATKRVRPAGIQAPGNFSDSWFESNGSLIRGNGSFPSGHAIAAFSV
                     ATVIARRYGNHRWVPYAAYGLAALVGFSRLSLSAHFLSDVFMGGALGYSISRSTVLRQ
                     "
     gene            5207..8026
                     /locus_tag="VFA54_11285"
     CDS             5207..8026
                     /locus_tag="VFA54_11285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS51438.1"
                     /translation="MGLLLAAIVFLPGTLCAQLTTGVIAGRIFGADRQPLAGARILVT
                     VGLGFSAVIYSSSQGEFSLVLPYGRCQLSVEVSPGRKPSGVAVFVAPSQTTAVNLEMD
                     ASGAVRAEALWTPGIWTDSTSGRAYPEGFGLPGLLLSREPSSVTEPLDFTGIHDNRPA
                     IESQRGFSWTDVQYRLQGLDATDSYQPGLPAVWPDTQALDEVIVRSGFAQAGSSDSGA
                     EVGLFLSQPGRSWHGAFSTANTGAALSWMNLPPPAFRGLVQQTDRFRWFTRDHLELGG
                     PLTRWADVYASASGQWSSQTEPLAAPGTDQRSRLLFGNVRSRLQAGGRDQLDLLYSSS
                     RIHLSDGGMPAGLEALTSNRMAPSFGLPGGFSGEPENDHFDFLQMGWTHLLSAGSGLG
                     AVQIRYGYSIAHLEASAIPGAQSRIELLGGAVSGAPPLGNLAVRPRHEIEALWRPTGF
                     HARGIRGQIVTGVDWKSSEPRNRFTAPSDLNLITANGAPAFVMEFDTPLDSRALVRSF
                     SAYLAGRVTVLPSLALDLSALADFPRGSLPAQSSPPGVFAPQRTFAARPDLIVWNSVS
                     PRAGFAWQVPYLRGLVLRGSYFRLYAPLAGRYLDFGNPNSFGGSTYEWETANPSQPFQ
                     PGEQGNLLLRFGGPYSAISSSLRRPYSDEFGAGAEFRFLRRSAASIHLFRRDDKNRIA
                     ALNVGVPAQAFTPVPILDPGPDGIPGTFDDQRLTVYAQDPATLGRDRYWLTNPAGLRM
                     LNAGLLAEAGAEWRGLALHASFVAEKSYGPTNPGDAVYENDPGVIGALFLDPNTAIHA
                     AGRSFMDRAYVGKIQASYRLPAAWSGIEAASVADYLDGLVFARQLLVTGLPQGPFLVA
                     ATVRGSPEGGNRAQYAINWNLRLSRQFALGSGKSLAVFFDVLNVTNAGQRLQENDRNG
                     PAFNLRLPVSIQPARFVRLGFRYEF"
     gene            complement(8322..8468)
                     /locus_tag="VFA54_11290"
     CDS             complement(8322..8468)
                     /locus_tag="VFA54_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51439.1"
                     /translation="MILKRSVRIAKKVQADGVWKFVSLTRNGNRCIWEERPGPYFLVW
                     HAAS"
     gene            complement(8564..8637)
                     /locus_tag="VFA54_11295"
     tRNA            complement(8564..8637)
                     /locus_tag="VFA54_11295"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8603..8605),aa:Gln,seq:ctg)
     gene            complement(9008..10000)
                     /locus_tag="VFA54_11300"
     CDS             complement(9008..10000)
                     /locus_tag="VFA54_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZS51440.1"
                     /translation="MQEKSSANSPLREQDNLDHLLVTTLEEPWYSSLVTQVRELLSRK
                     KEPPLELTSKPVPVKDIWGDYRYTRQAATSSLVTHAAVGVLLFTAFSHPAVRDAAKKT
                     ATLILPADISEYLPVAKPSIKPMGGGGGGGDRNPLPASKGRLPKFSLKQFVPPTTQIV
                     NENPKLPMEPTVVIPPEIQLPNVNLPQFGDPLAKIGPPSNGPGSGGGIGSGSGGGVGS
                     GTGPGVGPGSGGGVGGGVYRIGGGVSAPQLIHRVDPEYSEEARKAKHQGTVELYVEVE
                     PDGKAHKILVRRSLGLGLDEKAIEAVRQWTFKPAMKDGHPVTVGALITVHFRLL"
     gene            10208..13123
                     /gene="ileS"
                     /locus_tag="VFA54_11305"
     CDS             10208..13123
                     /gene="ileS"
                     /locus_tag="VFA54_11305"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZS51441.1"
                     /translation="MKIIDAAFSPAGGRGSPRDIMEDADLLNQGEIGLLDPKATINLP
                     KTAFPMKANLPQNEPKFLDRWQKEDLYGKLRQARQGASTYILHDGPPYANGNIHLGTA
                     LNKILKDFIVKSKTMAGFNAPYLPGWDCHGLPIEIKVDQDLGARKNRMSALEIRAACR
                     RYAEKYVDIQRRAFERLGVFGEWQNPYLTMSPEYEAAIAGAFVEMLSRGYVYKDLKPV
                     HWCWHDRTALAEAEVEYENHSSPSIWVRFPLLSGAEKVDPALAGRKVNAIIWTTTPWT
                     IPANMAIAFHPRFEYVAAEVENGEVYIVARRLLQATAERCGFAVKRILAEFPGERLEG
                     AAFRHPFLDRQSAGILAEHVTLEQGTGAVHTAPGHGLEDYLVGRQYGIPTYCPVDAGG
                     RFYHAEGAEGRLPGALIGKTVWEANPIVLELLRDRGALLASETIEHSYPHCWRCHNAT
                     IFRATDQWFIGMERNQLRSRTLEEIQRISWLPAWGQERISNMIATRPDWCISRQRVWG
                     VPIIVFYCERCQAPLTDRAILDRVVDLFREHTADVWYQREASELIPAGTRCGQCGGAL
                     FRKETDILDVWFDSGSSHLAVLGRRPDLPWPADLYLEGGDQYRGWFHSSLLIGMGIYG
                     SSPYRACATHGWTLDAEGRAMSKSLGNVIDPADIVKSHGAEILRLWVSSIEFSDDARI
                     SSEILERLSEAYRKLRNTFRYILGNLYDFDPSQDAVAAGQMLEIDQWALARADALVAE
                     CRRFYEQFAFHKVHHALIGFATVDLSALYFDILKDRLYTAAAKSIARRSAQTALYKLG
                     HILARLLAPLLSFTCEEVWGYLPKLPGEPESVHLALFPFPGEAIAGITEAQRKRLANW
                     DRLIEVRPAVLKALEQARREKFIGNALEARVRLSVDGELGHLLEEYARDLPMLFIVSE
                     VSLDPAGADHHLQVKVERASGVKCQRCWKYVTDVGVDPRWPAICAACAQAVEEMQRG"
     gene            13116..13667
                     /gene="lspA"
                     /locus_tag="VFA54_11310"
     CDS             13116..13667
                     /gene="lspA"
                     /locus_tag="VFA54_11310"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZS51442.1"
                     /translation="MADKQAGSRSNAAPAAAAPALRLLYLLLAAGVFIADRLTKTLIE
                     SGMTLHESRPVIPGFFYIVYAKNQGIAFGLFADSPTRFKTFVLIFLSSLALAGVAMLL
                     WTIDHSAPKVSAGLALILGGALGNLVDRVQGGGVVDFLDFFVSSYHWPAFNLADSAIV
                     IGGALLLLHMTRDSTRERSEQSS"
     gene            13734..14531
                     /gene="lgt"
                     /locus_tag="VFA54_11315"
     CDS             13734..14531
                     /gene="lgt"
                     /locus_tag="VFA54_11315"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZS51443.1"
                     /translation="MHPQLFRLGPFTIYSYGLLVALAFLAALLFASRLAQRAGLDPDR
                     VASLGVYVAAAAIAGAKVFLVLSDWRYYAQNPAQIFSLGTLQAGGVFFGGLVAALLAA
                     AWYMRRAGLPPLRTADTFAPAIALGHSIGRVGCFMAGCCWGRPANLPWSVTFTNPLAH
                     ELVGVPLGIPLHPAQIYESIAELIIFAILWLRVLRPHRGGSIIGLYLVLYSAFRFCVE
                     FVRDPADLSYPLGGPLSFTQWTALALAGGGIYLLSRRPAMTRSQAAL"
     gene            complement(14558..14881)
                     /locus_tag="VFA54_11320"
     CDS             complement(14558..14881)
                     /locus_tag="VFA54_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HZS51444.1"
                     /translation="MMRSKLLITAASFTMLLFLSACAGGGYYYAQVPPPPPPPRVIGV
                     VGLAPGPGYVWIDGYWAWDGHGYRTWVPGRWVRPPRRDVVWVAPHWERHGNGHRWVEG
                     KWRRR"
     gene            14943..17246
                     /locus_tag="VFA54_11325"
     CDS             14943..17246
                     /locus_tag="VFA54_11325"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZS51445.1"
                     /translation="MRLAALCLAVLYWFARPVSAQSIAAGRDQFQRLCAGCHGAEGAG
                     GERGPDIVSSRRTRRLNDQQLGELIRNGIPAAGMPAFPLKGGQMREIVAYVRSLIAPA
                     AESPAPGDAAAGEAFFFGKGGCAACHMVRGRGGISGPDLSDLGRRRRSAEIEQALRAP
                     GTVRTPGYEVATVRLGNGRSIRGFVRNESNYDLQLQDFAGTFHLLRQEQISGIAREKQ
                     SIMPALQAAPAERQNLLAYLSRLSGGPGPARQPVSEPTGAVPFADLLNPKPGEWPTYH
                     GRLSGNRHSPLRQIDVSNVSGLAPRWIFQIPNAGRLETTPVVVNGVMYVTAVNEVYAL
                     DAGTGRQIWHYARPRSRGLVGDAAGGINRGVAVLGDRVFLVTDNAHLVALHRVTGGLL
                     WDVEMADSRQNYGATSAPLAVKDLVISGVSGGDEGVRGFIAAYKAATGERVWRFWTIP
                     APGEPLAKTWAGKALEHGCGTTWLTGTYDPETDLLFWGAGNPCPDYNGDERQGDNLYS
                     SSVLALRPETGKLEWYYQYTPHDVHDWDAEQTPMVVDAEFQGRRRRLLLQANRNGFFY
                     VLDRTDGKLLRATPFVEKLTWASGIGPDGRPRLLPGAEPTPEGVPACPAVEGATNWMS
                     SAYNPDTGLFYLMALEKCNIYRKSPEVWEPGKSFYGGSTKDIPGEPGKKYLRAIYLQS
                     GKIAWEYPQIGPATTWGGVLATAGGLVFFCDDSGAFAAADAKTGKLLWHFPTSQSWRA
                     SPMTYMADGKQFVAVAAGSVILSFGLP"
     gene            complement(17310..18221)
                     /locus_tag="VFA54_11330"
     CDS             complement(17310..18221)
                     /locus_tag="VFA54_11330"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HZS51446.1"
                     /translation="MDQQELKNRLRGVIAFPVTPFQKDLSLDLEGLRRNVQFLVKHSF
                     SALVAAGGTGELYSLTPAEHRAVVEFCVAEAGDKLPVIAGVGINTQLAIEMTREAESV
                     GAAGILIFPPYYVNAHDDGIFEYYSAIAAATRLGCIIYSRDWANFSSAMVDRLSKIPN
                     MVAYKDGQGDIRLLQRIRARVGERLEWIGGVGDDLVPGYYSLGIRCYTSSISNIAPRL
                     CLQLHERATAFDSASVYRLMHNYVIPLYNIRARKRGYEVSVIKAAMQILGMPAGPVRP
                     PLTPVRPEEENELKTLMERFKPVLSKY"
     gene            complement(18203..19342)
                     /locus_tag="VFA54_11335"
     CDS             complement(18203..19342)
                     /locus_tag="VFA54_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51447.1"
                     /translation="MRTPGLKKTITAPSLAALAAALLGLPLSGQQLRITETAGIRRFS
                     FPVTAALPPQLAGRTVRLLRDGKPVPAQVRGGEIDLNVNLGPYERQEFSVVPQEMHAP
                     EAAGSIVSREGDQFTVSYGESLHYIVPDNLLGLLSAVKTPRWDYLRPGSQGLLLRFRD
                     DIIYRAGGFGPYGKATSASVVKQGPLDSVIEFSSTEALRGNYSVQSKVRMEFPRSKSW
                     VKTTWTVEDPNALLTGLGADLNLNLTSGPALVDFGAGTLVYTQLRPGQSAALIGQPQS
                     WHVLVGNEPYVTAKSGRAEGWAHVMDRERCTVVAVKDFGEREDRIEVSSDGRLQIWRS
                     FRGSGEKGMTFWLHFVRMPVEIGAATSAQSILAPLKIDWLWTNKN"
     gene            complement(19353..20654)
                     /locus_tag="VFA54_11340"
     CDS             complement(19353..20654)
                     /locus_tag="VFA54_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HZS51448.1"
                     /translation="MRTCLRICSFLLLGAWLTSGARAEYKPTAANLAAREWFQDAKFG
                     MFIHWGVYSVLGDGEWVMNNRKMTVEEYEKLAPQFNPVKFDPAAWVALAKAAGMRYIT
                     ITSKHHDGFAMFDSKLTDWDIVDRTPYKKDVLKMLADECHRQGIKLFFYHSELDWHHP
                     DYFPRGRTGWNTGRPESGDWYKYLDYMDGQLRELLTNYGEIAGIWFDGWWDKPNADWR
                     LEKTYGMIHSLQPAALIGNNHHHLPFEGEDFQMFEKDLPGGKTAEFNRDSKVGSLPLE
                     TCETINKAWGYNKGDLQFKSARDLIHYLVRAAGHNANFLLNIGPRPDGTIQPEFVERL
                     QQVGEWMKVNGDSIYGTRGGPIPPRDWGVTTQKGKKVYVHLLNLEDDVLALPKLPSKI
                     SAAALLSGNKVDYIENRLGVMLKVDHSQRDPIDTVVVLTLM"
     gene            20871..21488
                     /locus_tag="VFA54_11345"
     CDS             20871..21488
                     /locus_tag="VFA54_11345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS51449.1"
                     /translation="MPRSVEFFEHQFQRQVQMCEFELNPFERLALDYLQGAVLDLGCG
                     LGNLTLAAARRGCIVTAIDASPTAIARIRDAAERERLPVSALQADLADFQIQHDYDAV
                     VAIGLLMFFPKPQALRMLAEIKQHVRTGGYAIINVLIEGTTYMDMFTPGEYCLFRREE
                     LAERFSDWTIEAARYDEFPAPGETLKVFATVITRNPAVSSAHRPQ"
     gene            21675..23000
                     /locus_tag="VFA54_11350"
     CDS             21675..23000
                     /locus_tag="VFA54_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZS51450.1"
                     /translation="MIVRCLIAALAAGGIAAATSPSPVTFSRDVAPILQKHCQTCHRP
                     GEAAPMSFLTYRETRPWAKAIRESVLLRKMPPWFADPHFGKFANDRSLSQSDINTLAA
                     WAENGAPEGDPKDLPPPRKFIEGWNIGQPDAIVEMPSEFHVPASGTIDYQYIVIPAGF
                     TEDKWVQMAEVRPGNRALVHHMIAFVRPPGSKWLREAKPGVPFVPKKQSDRRESRRDG
                     DETEGEFLVGFAPGSMPEVLKPGQGKLIKAGSDFVFQMHYTANGKAGTDRTRIGLIFA
                     KETPAERVLTLAAQNRKFAIPPQDPNYEVRAGITFQDTAALLAMLPHMHLRGKDFEYR
                     LVYPDGRSEILLRVPKYSFSWQLSYYLQEPRMLTKGARIECTAHYDNSPNNPENPDPT
                     KEIRWGDQSWDEMMAGFFDVAFDAHKNPENLLKKETRPARGHGETAGGN"
     gene            23002..23673
                     /locus_tag="VFA54_11355"
     CDS             23002..23673
                     /locus_tag="VFA54_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZS51451.1"
                     /translation="MRGRIGARCLLLFSLGAGLCRAHDPITTKITWTREISRIFYRRC
                     FACHGKEGPSMPLLTYEQTRPWAKAIKEAVLERRMPPWGPVKGFGGFKNDASLSEVEI
                     ELIVNWVEGGAPEGELMYLAPPPDFIPKPPDPPPAEPVPIKGLVRLDREVTAIGVEPS
                     GSVQATAHIPGGGIAPLIWIRDFRPGQPKTYYFREALKLPKGTEIRCEGAAARLLIAA
                     SQPAQ"
     gene            complement(23645..24508)
                     /locus_tag="VFA54_11360"
     CDS             complement(23645..24508)
                     /locus_tag="VFA54_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HZS51452.1"
                     /translation="MRRRRFLGQILGAPFLVPFLWAQAPEPPVEWVCPMDPEVRGKGP
                     GKCPKCNMDLEPDLPDPVEYPIKTAIFPRAFHAGQKVRLEIQVLDPVTGRPVQKFRAV
                     HEKLFHLFLISQDLQFFLHEHPQLTGDSVFRLETRLPKPGRYRLLCDFYPEGGTPQLV
                     PATLIAPGAGAQPQLAPDLVSKHAANLAVELSTEPAPPIAGQKTLLFFRLKPAEGLEP
                     YLGVWGHLLAASEDLIDLIHEHPTIAHGGPQVQFNLIFPRPQMYRIWVQFQRLGKVNT
                     AVFTVPVAKLQ"
     gene            24818..26512
                     /locus_tag="VFA54_11365"
     CDS             24818..26512
                     /locus_tag="VFA54_11365"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HZS51453.1"
                     /translation="MPAKKLRSQEWFGGSAYEASVRRAWLRSEGFGAQVFGGKPVVGI
                     CNSWSELNNCNAHLRQVAEAVKRGVWAAGGFPLEFPTISLGELFMKPTTMLFRNLMAM
                     DVEEMIRANPIDGVVLLCGCDKTTPAQLMGAASADIPAIMVTGGPMLAGNWKGNVLGS
                     GTDGRRYFQELRAGRITDEQWCEIEGCIARSAGHCTVMGTASTMAALAEALGMTLPGN
                     AAIPAVDSRRYQLAEASGARIVEMIRQDLRPSRILTRAAFENAIKTDMAIGGSTNAVV
                     HLVAIAGRLGIELPLSEFDRISRQTPFLVNVKPSGAYVMEDFFYAGGLPAVLKELAPL
                     LDTTVLTATGRTLEENLRSVERSTNRDVIRPLDQPLAPEGGTAILYGSLAPGGAVIKQ
                     TAASPRLLRHRGRAVVFASYDDLRARIDDPALEVDANSILVLQNAGPKGGPGFPEWGH
                     LPIPAKLLKQGVDDMVRISDARMSGTSFGTDVLHVAPESAVGGPLALVRTGDEISLDV
                     EARRLDLCVSQGELNRRRAQWRPPAPHYTRGYGKLFLDHVTQADQGCDFDFLRACG"
     gene            complement(26534..26944)
                     /locus_tag="VFA54_11370"
     CDS             complement(26534..26944)
                     /locus_tag="VFA54_11370"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZS51454.1"
                     /translation="MTAVALFLIGRILFGVFFLASGFHHFADVHNMSAYARSKGTPAP
                     WLAVIATGVLLIAGGASILLGAYPVLGIVLLGIFLVGVSFVIHGFWTIQDPQQRAAER
                     VNFLKNMALLGALLMFLLIPVPWPLSVHFGLPIP"
     gene            complement(27351..27902)
                     /locus_tag="VFA54_11375"
     CDS             complement(27351..27902)
                     /locus_tag="VFA54_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZS51455.1"
                     /translation="MRKSMLVAGGAILIAIVAGRAQQQEVEKLAPFYPTPEAVVQKML
                     ELGELKKGEWHYDLGSGDGRVVIMAAQKFGAHSVGFEIDDSLYQRSMAKIKDLGLEKL
                     ATIRKQDLMTADFSKPDLITVYLLPTSNDKIQPLLEKSMHKGSRVVSHDFEFRGWKAE
                     KTINIEDDDQGDIRTHTLYLYRR"
     gene            28002..28499
                     /locus_tag="VFA54_11380"
     CDS             28002..28499
                     /locus_tag="VFA54_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HZS51456.1"
                     /translation="MGETAMELFTVKITIPEAANVIVGQTHFIKTVEDLYEAIVTTAP
                     GMKFGVAFNEASGACLTRVDGNDEELQRAAAENALSIGAGHVFVIMLRDGYPINVLNR
                     IKDVSEVCAIYCATANPLEVIVAQTGQGRGILGVVDGSPPKGIEGPSDAAWRRDLLRK
                     IGYKR"
     gene            complement(28496..28963)
                     /locus_tag="VFA54_11385"
     CDS             complement(28496..28963)
                     /locus_tag="VFA54_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS51457.1"
                     /translation="MKNKIWPGLLISAAVGVTLAQTAVLSRHSRSPLERAGAVSRSEP
                     NPYQGNREAERAGAKLFRRYCAACHGEDARGNGKAPPLVSPEVGGAMPGALFWVLRNG
                     SLASGMPSFSRLPEAQRWQIIAWLQRLNAAGSATASPPENAGALRSWGPEHAP"
     gene            complement(28967..>29190)
                     /locus_tag="VFA54_11390"
     CDS             complement(28967..>29190)
                     /locus_tag="VFA54_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51458.1"
                     /translation="ELYGGLGTHEHFGSVADMAHYLAPVVAWQLPNGASLQISPAFGL
                     TGSSVPFLLRFGVSYEVAQFGRVVSRMFR"
BASE COUNT         5434 a         9081 c         8915 g         5760 t
ORIGIN      
        1 cggaccgctc gaaatgaccg ccggctggcc gctcgccacg ctgttcaccc tggccgtggc
       61 ggcgagcatg gccgagctgt gctctgcgtt ccccacctcc ggcgccatgt accactgggc
      121 cgcccacctg ggcgggccaa tctgggggcc ggcctgcggg tggtttgtag cgtggcttaa
      181 tatcctcggc ttggccgcgg ctctggccgg cattaactac agttgcgctc aatttgtgct
      241 tccctttcta ggcattgctt cgacctacag caacctgctg gccgcgttcg gccttatcct
      301 ggtcacccaa gcgctgctaa atcactacgg cattcgcctg gtggcgatcc ttaacgattg
      361 cagcgtcacg gtacacattg ccggagtggt ggctctggtg gcggcccttt tgctgctcgc
      421 ccggctccag ccggcgcgat tcttgttcca ggctacaact accaacggac gtttcccgta
      481 cgggtgggcc tttgttctag gtttgctcca ggcgcaatgg acctacaccg gctttgacgg
      541 ctcggcgcat gtagcggaag aaacgtccga cccgcgccgc cgcgctccct ggggcatcgt
      601 gctttccgta gcggtatcgg gaatcgtggg atacctgctg attttgagcc ttacgctcgc
      661 aatccgggcg attccctcgg ttctggaaac gcgcgatgca cagggcaatc cgctcccagc
      721 ggcgatcgtg attttgcagc aagcgctcgg cgcccgggca ggtaatgcca tgtcggcgct
      781 ggcgtcgatg gcaatgtggt tttgcggcct ttcctgcgtg acttctgcgt cgcgcgccat
      841 tttctcgctg gcccgcgatc agggcgcgcc attctccgcc cttctccggc gggtgagtcc
      901 ccgccacggc acgccggctg cggcgatctg ggttttcacc ggggcatcct tcgctgccat
      961 gctctggacg ggagccatac cggttgtgac ttccttgagt accgtagctt tgtacgccgc
     1021 ctacattgtc ccgatcatgc tggccttgcg gagccgcagc tcgggctggc agcattccgc
     1081 cccgtggtcg ctggggcgct ggggcgcgcg cgtcaacctc gtggccattg tatacactct
     1141 gtttatttgc gcggttctgg tgatgcctcc caatgtgctg gcggggaaaa cgttccttgg
     1201 gttgatggca ggactcatct tgctttacgc cttggtggtt cgccgcaggt atcgcggccc
     1261 tcaatggagc cgcggtgagg caattgctcg cggaggcgcc gtgtagcgcg gcggaatatt
     1321 tgaaccggcg agcccggttc aggcaggcgc cggcgagcgc tggttttttt ggaacagatc
     1381 aaacaaagca gacgggtctg ggtggccctc tcatgaatcc agtactggtc aaacggaagt
     1441 tcgacgagcg aggcaccaag cgcgtcaagc tggccctggt cgatatcgac ggcgtgctgc
     1501 gcgggaagta tgtcagcctg gacaaattct ggtccgccgt cgaatcggac ctcggcttct
     1561 gcgatgtgat tttcggttgg gacatctccg acgccctcta tgacaatgtc cgattcaccg
     1621 gctggcacac gggatatccc gacatgcgcg cccgcatcga tacgaccaca ttccggctga
     1681 tcccctggga ggccgacacg gcgttctttt tgctcgacct catcgataag aaaggcgcgc
     1741 cgatcgcggt ctccccgcgc caggtgtttc ggcaagtcgt agagcgcgcg gcctcgctcg
     1801 gctacactcc gtatttctcc gctgaatatg aatttttctt tttccgcgag acgccgcaga
     1861 gcctgcgcga gaagaattac cggaacctta gctccctgtc gcccggaatg ttcggctaca
     1921 gcgtgctgcg ggctgcggct aattcggagt tcgtgctcca agtgatcgac cagcttgccg
     1981 agttcgacgt gcaactcgag ggcttccaca ccgaaaccgg ccccggcgtc tacgaggccg
     2041 ccgtggcagc agctccagga cttcaggggg cggatagagc ggctttattt aagacggccg
     2101 tgaaggaaat tgcggcccgg cacggcctga ttccgacgtt catggcgaag tggaacgccg
     2161 atctgccggg atcgggcggc cacatccatc agagcttgtg ggatagtaag aaaggccaga
     2221 acctcttcgc cggggaaaat tgcgggatgt cgcaattgat gcagcactac atagccgggc
     2281 tggcagcgag tctgccggag ctgaccgcga tgatgtgccc aactattaat tcctacaagc
     2341 ggatggtccc cggcttttgg gccccggtaa acgctacctg gggcctggac aaccggacgt
     2401 cggcggtgcg cgccattgtg ggagaggata agtccacaag ggtcgagacc cggctttccg
     2461 gggccgacat gaatccgtac ctggccatgg ctgcctcgct cgccgccggc ctcgatggca
     2521 tcgaacggaa actcgaactg ccgcctccgg ccaaggacgc gtacgccgcc aatgccctgc
     2581 ctctgcccgc gaacctggcc gaagccaccc gcctttttcg ccagagctcg ctcgctcgca
     2641 aggcttttgg cgaagaattc gtcgaacatt atgcagctac ccgcgaatgg gaagtgcggc
     2701 aatatgaacg ggcggttacc gactgggagt tggcccggta cttcgaagcg gtctaagcgc
     2761 ccgccatttc gctgcaaacg cggctcaggt atcttgcgcc ggcccttgtc tccgtagggc
     2821 cgattccagc gatccattgg aactcgtcac gcaaggttag gccacgggcg catacaccag
     2881 agtgatattg cctcactttt cgcaagcggg gccagaacat cagaaggcca agaccggcgg
     2941 atggttgacc cggaaaccgt aattgagcag aatgaccatg tacctttcgg cccttgcaat
     3001 ctccggaggc cagctctgaa ggggccagtc ttactctgag gtactcagtg gcgcagcgat
     3061 tcccggttcc ggcggctgca tccggtccgc tcaatacggc tggcgaatcg tttgcccaag
     3121 ctcgtgtgca gattggcgga ggtgcctgat gttttgttca aactgtggag cgcccctcag
     3181 cggcgctttc tgcggcaact gcgggcgtcc cgccccgcag gctgctcaac ccgttgcaaa
     3241 gccggccgcg ccggctggtt ccacgccgcc ggtgcaatcc gggggcgcgc tgaaagtcgt
     3301 cctgatctgt ttcctctgcc ttggtgtcct gggtttgatg agcgcggcag gactgtatta
     3361 cgccgccacg cgcgtcaagt cccggctgct ttccgccgca ggcgtggcgc gtgcgacgga
     3421 acacggcagg tcttccgccg gttccaaaat cgacgcctgc gcgctgcttt ccaaagagga
     3481 acttggggaa ctgctgggaa tccacgttga tcgcgcccaa cccattgccg acggcgggga
     3541 gcctggctgc gcctttttcg cgtcgccgga aacgctccgg caactggccc gcgactcgct
     3601 ctccaaagcg cccgagcagg tcaagactgc cgaagcgaag ggccgggtga aaggcgacaa
     3661 tccgctggcg aatgtgaata cgtctaccat ccagggcctg gaaggcatcg tcaagacgat
     3721 cgccgcggcg ggtgacgcca gccagggcgg cggcaaggtc ttctcattca ccgtggaccc
     3781 gaagttcgac gcggaatcct ggcccctgtt cctgaaaacc atgagcgtcg tccccggatt
     3841 cgagcctttg gcgggtgtcg gcgatcacgc cctgatggga cctttcggcc aaattctata
     3901 tgtgcaaaaa ggcaatgtga aagtaacgct ggaattgacc agcgtgcccg acgcgcacgg
     3961 caaaggcgtc gatcttgcgc gcaaaatcat ctcccggctg tgacagatct gcctcgaccg
     4021 gagcgcgggc agcccgcggc aaaatatcgg ccctcgatca agaccgggaa agaatgatcg
     4081 tgttgtggga tgtctgcccg ttccggtctt tccagaaacg ttgagaggcg gaacaacgca
     4141 gccggcctcc ggcaggcgct ggctccattc atacttgatt ttgttcttga agttttactc
     4201 tcaaaaagct agatgagatt gtgacttcgg attgcagtga tatggctttc tatctctgta
     4261 agcgagtatg actgatttgc ccgggaagtt tttccgcccc cctgaggcaa ccgattcctc
     4321 ctgggcctcg tctttcggtc attgtcggcg cacggagcgc tgtctatgaa ggctgcggtt
     4381 gtgttaatcg cgttatctgt tcttgccatt ccgttcccgg cggcaactca aaccgtggaa
     4441 ccggggcaaa caccggccgc tgatgccccg accagcgctc cacttgtaac acaggagcca
     4501 tcaccggttt cgccggcagc ggatcgtccc gtatcttgga aggggctgcc cctgaacctg
     4561 ctgagcgatc agaagcgcat ttggtcgttt ccggcccggc ttgcgcgcgg gcaaagctgg
     4621 attcctgcca ccgcagtcgt gggaaccacc accggcttgt tcttcgtgga ccccaccgag
     4681 gctgcctatt tccaccgcac gccggcgttt catggctgga ataacatttt cacgagcaat
     4741 gccacgctgg cgggcacgat cgtcgtaccc gcctcgcttt atgccgccgg gctggtccgc
     4801 aaagacccca aaatgcagca tacggccctg ctcgcgggcg aggctgtggc cgacgccgag
     4861 attgttacaa cggttctgaa ggacgcgacc aagcgggttc gtccggctgg cattcaggct
     4921 cccgggaatt tttcggactc ctggttcgag agcaacggct ccctgattcg cgggaacgga
     4981 agttttccct ctggccatgc catagcggcg ttctcggtgg ccacggtcat cgcccggcgc
     5041 tatggaaatc accgctgggt tccttacgcc gcgtacggct tagccgcact ggtgggattt
     5101 tcgagacttt ctttatcggc ccactttttg tcggatgtct tcatgggtgg cgcccttggt
     5161 tactcgatca gccgttccac ggtgctccgc cagtagccgc cccccggtgg gactccttct
     5221 ggcggccatc gtgttcctgc cgggaacgct ctgcgcgcag ttgaccacag gcgttatcgc
     5281 cggaagaatt ttcggcgccg atcggcagcc gctagccggg gcgcggatcc tggtcaccgt
     5341 cggcctcggg ttcagcgcgg tcatttactc gagctcgcag ggcgagtttt ccctcgtgtt
     5401 accttacggg cggtgccaac tgtctgtgga ggtatcgccg ggcaggaaac cctccggcgt
     5461 ggccgttttc gttgcgccgt cgcagaccac ggcggtcaat ctggagatgg acgcttcggg
     5521 agcagtccgc gcggaagcgt tgtggacacc aggcatttgg accgattcta cctccgggcg
     5581 cgcgtaccct gagggtttcg gcctgcccgg tcttctgctg agccgggagc cctccagcgt
     5641 gaccgagccc ctcgatttca ccggcatcca cgacaaccgg ccggcgatcg aatcgcagcg
     5701 gggattctcg tggacggatg tgcagtacag gctccagggc ctcgatgcca ccgactctta
     5761 ccagccgggc ctccccgccg tatggcccga tactcaagct ctcgatgagg ttatcgtgcg
     5821 aagcggcttt gctcaggcag gttcgtccga tagcggcgcg gaggtcgggc tttttctttc
     5881 ccagccgggc cgttcctggc atggggcgtt ttccacggca aacaccggcg cggcgctttc
     5941 ttggatgaat ctcccgccgc cggcttttcg cggacttgtg cagcagacag accgttttcg
     6001 ctggttcact cgcgaccatc tggagcttgg cggcccgcta acccggtggg cggacgttta
     6061 tgcatccgcc agcgggcaat ggtcctcaca gaccgagccg ctcgccgcgc ctggaaccga
     6121 tcagcgcagt cggctgcttt ttgggaacgt tcggagccgc cttcaagccg ggggcagaga
     6181 ccagttggat ctactgtaca gcagctcgcg aatccatctt tccgacgggg gcatgccggc
     6241 tggcctcgaa gccttgacca gcaatcggat ggcgccatca ttcgggctgc caggcggttt
     6301 ctcgggcgaa ccggagaacg atcatttcga ttttctccag atgggctgga cacatcttct
     6361 ttccgcgggt tccggtctgg gcgcagtcca gattcgctac ggatattcga ttgcacacct
     6421 cgaagccagc gccatccctg gcgctcagag ccgcattgag cttctgggcg gcgcggtctc
     6481 gggagcgcct ccgctgggga atctcgccgt ccggccgcgc catgagatcg aagccctgtg
     6541 gcgtcccacc ggcttccacg cgaggggcat tcggggccag atcgttaccg gagtggactg
     6601 gaaaagctcc gagccgcgca accgctttac agctccttcc gatttgaatc tgatcaccgc
     6661 caacggtgcg ccggcgttcg tgatggagtt cgacacgccg ctcgattccc gcgcgctggt
     6721 gcgatctttt tccgcatacc tggcaggccg cgtcacggtg ctgccttcgc tggcgctcga
     6781 tttgagcgct ctggcagact ttccgcgcgg ctcgctcccc gcacaatcga gccctccggg
     6841 cgtgttcgca ccgcagcgga cgttcgccgc ccggccggat ctcatcgttt ggaacagtgt
     6901 ttccccgcgg gccggtttcg catggcaagt cccttacttg cggggcttgg ttcttcgggg
     6961 ctcctatttt cgcctgtatg cgccgctcgc tggcaggtac ctggacttcg gaaatccgaa
     7021 cagttttggc ggctctacct atgaatggga aacagccaac cccagtcagc catttcagcc
     7081 cggcgaacaa ggcaatttgc tgctgcgatt cggcgggccc tactcggcaa tctcatcctc
     7141 acttcgccgg ccttactccg acgaattcgg cgccggcgcg gagtttcgtt tcttgcgtcg
     7201 aagcgctgcg agcattcacc tgtttcgacg cgacgataag aatcgcatcg ccgccctgaa
     7261 cgtcggggtg ccggcccagg cgtttacgcc cgtgcccatc ctggatccgg gacccgatgg
     7321 gattcccggc acgtttgacg accaacggct aaccgtctat gcgcaggatc cggccacctt
     7381 gggacgagac cggtattggt tgaccaatcc cgcggggctg cggatgctca atgctggcct
     7441 gctggcggaa gcgggcgccg agtggcgggg gctggcgctg cacgcgtctt tcgtggccga
     7501 aaagtcgtac ggccctacca atccgggcga tgccgtctac gagaatgatc ccggcgtgat
     7561 cggcgctctc tttctcgatc ccaacaccgc gatccatgcg gcgggccgaa gcttcatgga
     7621 ccgggcgtat gttggaaaaa tacaagccag ttaccggctg ccagcggcct ggagcggcat
     7681 cgaggcggcg agcgtcgcag attacctgga tggacttgtg tttgcccgcc agctactcgt
     7741 tacaggcctc ccgcagggac ctttcctggt ggccgctaca gtgcggggca gtccggaggg
     7801 aggcaatcgc gcccagtatg cgatcaattg gaatctgcgg ctgagccggc agttcgcact
     7861 cggctcggga aaaagcctgg ctgtcttctt cgacgttctc aacgtgacga atgcagggca
     7921 gaggcttcag gaaaacgatc ggaacggtcc tgcgttcaat ttgcggctgc cggtttcgat
     7981 tcagccggcg cgtttcgtac gtctcgggtt ccgctacgaa ttttgatctt cgccaattgc
     8041 tatgcctgcg tcctcgaggt ctgtatcacg gcgaacacgg cgatccacca ttgttctatg
     8101 agcccgtaag gacgcacgat ttagcgcggg gtcggcggaa gagagggccg gcgcagctct
     8161 ctagaaattc gaccggtcgg cagcgccagc taggggcggc cggcaatcgc ggcttgggta
     8221 caatcaggag acgccctggg cgagcttccg ctgcgctagg ggcggccggc aatcgcggct
     8281 tgggtacaat caaatctcgc atttttgttt ttccgctaac gctagctcgc tgcatgccag
     8341 acgagaaaat atggcccggg ccgctcctcc cagatgcagc ggttcccgtt gcgcgtcagc
     8401 gagacgaact tccagacgcc gtcggcctgg accttcttgg cgattcggac cgagcgcttc
     8461 aggatcatcg gccgcctccc gcgtgcgggg ttgtcaaaac atggacacca aggcccgaac
     8521 cggtcttgga tatggttggt tctaaagaaa actaccttgg aaatggctgg gaggcaggga
     8581 ttcgaacccc gataagcaga tccagagtct gccgtgttac cgttacacca cctcccatcc
     8641 ggccaacgta ggactcccgc gcggatgagc gagttcaccg gatcaggcgc gtagttcgag
     8701 tataacgcga cggccggtaa cccgtccaac attgcaccat caagagggca atggaatcgg
     8761 ctgaacgact ctcgctccct ccggcggatc gctacccttc cgaaataacg cgaaactggt
     8821 gtaattggag aaggaaaacg taacaagaac cgccgcgggg atgtgccgcc gggtgaccgt
     8881 tgtgagggag tcgagggcct ctagaggaac aggagaacga agaaactcac cgccgtgatc
     8941 ggaaaccgga tgccaaccgt acaccggagg actgctactc tgcccagctc agcgcagtcg
     9001 cggcggatta caacaggcgg aagtgtaccg tgatcaaagc gcccaccgtc accggatgac
     9061 cgtccttcat ggcgggcttg aaagtccact ggcggacagc ctcgatcgct ttttcgtcta
     9121 accctaaacc gaggctgcgc cgtaccagaa tcttgtgcgc tttcccgtcc ggctctacct
     9181 ctacgtagag ctcaacagtg ccctgatgtt tcgccttgcg ggcctcttcc gagtattccg
     9241 gatcgactcg atgaatcaac tgcggcgccg agactccgcc gccaatccta taaaccccgc
     9301 cgccaacacc gccgccggat cccggcccga cacccggccc ggtcccggaa cccacgccgc
     9361 ctcccgagcc ggagccaata ccgccgccgg agcccggtcc attggagggc ggcccaatct
     9421 tagccagcgg gtctccgaac tgtgggagat tcacgttcgg cagttggatt tcaggtggaa
     9481 tgacgaccgt tggctccatc ggcagcttcg ggttctcatt gacgatctgt gtggtcggtg
     9541 gaacaaactg cttcagcgag aacttaggca atcttccttt gctggccggc agcgggttgc
     9601 ggtcgccgcc accgccgccg ccgcccatcg gtttgatgga aggcttcgct accggcaagt
     9661 attcggatat atctgccggc aaaatgagtg tcgccgtttt cttggctgcg tctcggaccg
     9721 ccggatggga aaaggctgta aacaacagca cgccaaccgc cgcgtgggtg acaagcgagg
     9781 atgtcgccgc ctggcgggtg taccgatagt cgccccagat gtccttcacc ggaaccggct
     9841 tggacgttaa ttccagaggc ggctcttttt tcctgctgag cagttcccga acctgtgtta
     9901 ccagggagct gtaccagggc tcctccagcg tggtgaccag caggtgatcc aaattgtctt
     9961 gttcccgaag gggggagttt gcgctgctct tttcttgcat gcctgtatcg ggcttctcct
    10021 cagttggcga gcctgagcct tgcacctggg cacgctctga ctgattcgac gccgctactc
    10081 cgcaagacgt tacgcctttt ggaatctgcc ctcatgttaa cacatccgtc aaatgtatca
    10141 acgttataac aaggcgacaa gacgcgcgca agcaggggag ctgtaaattc ggcagcggcg
    10201 ttttctattg aaaatcatag atgctgcgtt ttctccggcg ggcggccgcg gatcgccgcg
    10261 cgatataatg gaagacgccg accttctcaa ccagggggaa attggattgc tcgatccgaa
    10321 ggccacgatc aacctcccga aaacggcgtt cccgatgaag gcgaatctgc cgcaaaatga
    10381 accgaaattc ttggaccggt ggcagaaaga agacttgtac ggcaagctgc gccaggcgcg
    10441 ccagggcgca tccacttaca ttttgcatga cgggccgcct tacgccaacg gcaacattca
    10501 tttgggcacg gctctgaaca agatcctcaa agacttcata gtgaagtcga agactatggc
    10561 gggctttaac gcgccgtacc tgccgggatg ggactgccat gggctgccga ttgaaatcaa
    10621 agtagaccag gatctcggcg cgagaaagaa tcgtatgagt gcgctggaga tccgggccgc
    10681 ctgccggcgc tacgccgaaa agtatgtgga catccagcgc cgggccttcg agcgcctcgg
    10741 ggtttttgga gaatggcaga atccctatct caccatgtcg ccggagtacg aggcggctat
    10801 cgctggggcc ttcgtggaga tgctgtcccg aggctatgtt tacaaggatc tcaagcccgt
    10861 acactggtgc tggcatgatc gtacagccct ggccgaagct gaagtcgagt acgaaaatca
    10921 ctcgagcccg tccatctggg ttcgctttcc gctgctttcc ggtgctgaga aagtggatcc
    10981 cgctcttgcc ggacgcaagg tgaacgcgat catctggacg acgactccgt ggaccattcc
    11041 cgctaacatg gcgatcgcct ttcacccgcg attcgagtat gttgcggcgg aagtggagaa
    11101 cggggaagtc tacatcgtcg cccgccggct tttgcaggcg acggcggagc gatgcggctt
    11161 cgccgtgaaa cgcattctgg ccgaatttcc cggtgaaagg ctggaaggcg ccgccttccg
    11221 gcatccgttt ctcgaccgcc aatcagccgg gattttagcc gagcacgtga cgctcgaaca
    11281 gggcacaggg gcggtgcaca cagcgccggg tcacggactg gaagactacc tcgtaggccg
    11341 ccaatatgga atccccacgt attgtccggt agacgccggc ggcaggttct accacgccga
    11401 aggcgccgag ggaaggctgc cgggtgcgct gattggcaaa accgtttggg aagctaatcc
    11461 gatcgtgctc gaactgctgc gcgaccgggg cgctctgctc gcgagcgaga ccatcgagca
    11521 cagctatccc cactgctggc gctgccataa tgcgaccatc ttccgcgcta ccgaccagtg
    11581 gttcatcgga atggagcgca accagcttcg cagccgcact ctcgaggaga tccagcgcat
    11641 ttcgtggctg cccgcctggg gccaggagcg catttcgaat atgatcgcta cccggccgga
    11701 ttggtgcatc tcgcggcagc gcgtgtgggg cgtgccgatc atcgtcttct actgcgagcg
    11761 ctgccaggct ccactcaccg accgggccat tctcgaccgc gtcgtagacc ttttccgcga
    11821 gcatacggcc gatgtatggt accagcgcga ggcaagcgag ctgattcccg ccggaaccag
    11881 gtgcggccaa tgcggcggag cgctgtttcg caaagagacc gacattctcg atgtgtggtt
    11941 cgattccggc tccagccacc tcgccgtgct cgggcgccgc cccgatctgc catggccggc
    12001 cgatctctat ctggaaggag gcgaccaata ccgcggctgg ttccatagct cgctgctgat
    12061 tggaatgggt atttacggca gctcgcctta ccgcgcctgc gccacgcacg gctggactct
    12121 cgatgccgag gggcgggcga tgtcgaaatc tttgggcaat gtgatcgacc ccgccgacat
    12181 tgtcaaatca cacggggcgg aaattctgcg gctttgggtg agctcgatcg aattcagcga
    12241 cgacgcccgc atatcctcgg aaatactgga acggctttcc gaggcatacc ggaagctgcg
    12301 caacacattc cggtacatac tgggcaatct ctacgacttc gacccgtccc aggacgcagt
    12361 ggcggccggc cagatgctgg aaattgacca gtgggctctg gcccgcgccg acgccctggt
    12421 ggctgaatgc cggcgttttt acgagcagtt cgcgtttcac aaagtgcacc acgcgctcat
    12481 cggcttcgcc accgttgatc tcagcgctct ctacttcgat attctgaagg atcgcctgta
    12541 cacggccgct gccaaatcca ttgcccgccg cagcgcgcag accgcccttt acaagctggg
    12601 ccatattctg gcccgcttgc tcgcccctct gttgagtttc acctgcgagg aggtgtgggg
    12661 gtatttgccg aaattgccgg gcgagccgga aagcgtgcat ttggcgttat ttccctttcc
    12721 gggagaggcg atcgccggga tcaccgaagc gcagcggaaa cgtctggcca actgggatcg
    12781 cctaatagag gtgaggcccg ccgtgttgaa ggcgctcgag caagcccgcc gggagaaatt
    12841 catcggcaac gctttggaag cgcgggtgcg cctttcggtc gacggggaac tgggacactt
    12901 gctcgaggag tacgcgcgcg acttgccgat gctttttatc gtttcggagg tgtcgctgga
    12961 ccccgccggc gcggaccacc atctgcaagt gaaggtcgag cgcgcctccg gcgtgaagtg
    13021 ccagcggtgc tggaaatatg tgaccgatgt gggggtagac ccgcggtggc ccgcgatctg
    13081 cgccgcatgc gcgcaagcag tggaagagat gcaacgtggc tgacaagcag gcaggaagcc
    13141 ggagcaatgc ggccccggcg gcggccgctc ctgctctgag gctgctctat ctattattgg
    13201 cggccggcgt tttcatcgcc gaccggctca ccaagacttt gattgaatcg ggcatgactc
    13261 tccacgagag ccggcccgtc atccccggtt tcttctacat tgtttacgcc aagaatcagg
    13321 gcatcgcttt cgggcttttc gccgactcgc cgactcggtt taagactttc gttctcattt
    13381 tcctctcgtc gctcgcgctg gcgggcgtgg ccatgctgtt gtggacgatt gatcattccg
    13441 cgccgaaagt gagtgcaggg ctggcgctta ttctgggcgg tgcgctgggg aacctggtgg
    13501 accgcgtcca gggcggcggc gtcgtggact tcctcgattt cttcgtcagc agctatcatt
    13561 ggcccgcgtt caacctggcg gactcggcca ttgttatagg cggcgcgctg cttctgctgc
    13621 acatgacacg ggattccaca cgggagcgga gtgaacaatc gagttgaaaa cggccgccgc
    13681 gcgggggttc gccccgggag ccctcgggcg cctttcccgc tgatggcaag ctgatgcacc
    13741 ctcaactatt tcgtctggga ccatttacta tttacagcta cgggctgttg gtagcgctgg
    13801 cgtttctcgc cgcactgctg ttcgcctcgc ggctcgcgca gcgcgccggg ctggatccag
    13861 accgcgttgc cagcctcggc gtttacgtag ccgcggcggc tatcgcgggg gccaaagttt
    13921 ttctcgtcct gagcgactgg cgctattacg ctcagaatcc cgcccagatt ttttccttgg
    13981 gcacgttgca ggcgggcggc gtcttttttg ggggcttagt agcggcgctg ctagccgccg
    14041 cgtggtacat gcgccgcgcg ggactgccgc ctctccgtac tgccgatacc ttcgccccgg
    14101 cgatcgcgct cgggcattcc atcggccgag tgggctgctt tatggcggga tgctgctggg
    14161 gacggcccgc caatctgccc tggtctgtaa cctttaccaa cccgcttgct cacgagctgg
    14221 tcggcgtgcc gctggggatt ccgctgcatc ccgcgcaaat ctacgaatcc atcgccgaac
    14281 tgattatttt cgccatcctt tggctgcgcg ttctgcgccc gcaccgcggg gggtcgatca
    14341 tcggactcta tctggtgctt tactctgcgt ttcggttctg cgtagaattt gtgcgggatc
    14401 cggccgatct gtcgtacccc ttgggaggac cgctctcatt cacacagtgg acggcgctgg
    14461 cgctcgccgg aggcggcatc tatttgcttt cccggcggcc tgccatgacg cgctcgcaag
    14521 ccgccctctg ataccggcgc aatacgcccc gcggcggtca gcggcgccgc cacttccctt
    14581 ccacccagcg gtggccgttg ccatgccgtt cccaatgcgg cgcgacccag accacatccc
    14641 ggcgcggcgg ccgcacccag cggcccggca cccaagtccg atacccatgg ccgtcccatg
    14701 cccaatagcc gtcaatccat acatagccgg gcccgggcgc caggcctacg actccaatca
    14761 cgcgcggcgg cggcgggggc ggcggaacct gcgcgtagta atagccgcct ccggcgcaag
    14821 ccgacagaaa gagcaacatc gtgaaactgg cagccgtgat aagtagtttg gagcgcatca
    14881 ttattttggc ctcccacttc ttgaaacgcg gcggcggacc ggccggttgt taaaatccct
    14941 tcatgcggct tgcagctttg tgtcttgctg tcttgtattg gtttgcccgg cccgtttccg
    15001 ctcagagcat cgccgccggg cgggatcaat tccagcggct gtgcgccggt tgccacggcg
    15061 ccgaaggagc gggtggagag cgcgggcccg atattgtttc ctcgcgccgc acgcgccgcc
    15121 tgaacgatca gcaactcggc gagctgattc gcaacggaat tcccgccgcg ggcatgcccg
    15181 cgtttccgct aaagggcggg cagatgcgcg aaatagtggc ctacgtgcgt tcgctcatcg
    15241 ctccagccgc cgagtctccg gcgcccggcg acgccgccgc gggagaggct ttcttcttcg
    15301 gcaagggcgg atgcgccgcg tgccacatgg tgaggggccg gggaggcatt tcggggcccg
    15361 atctttccga tctcgggcgg cggcgaagaa gcgccgagat cgaacaagcc ttgcgcgcgc
    15421 cggggaccgt gcgcactccg ggatacgagg tcgccacagt ccggctgggc aacgggcgaa
    15481 gcatccgggg attcgtccgg aacgaaagca actatgatct gcaattacaa gatttcgccg
    15541 ggacgtttca tctgctgcgc caggagcaga tcagcgggat tgcgcgcgag aagcaatcca
    15601 tcatgcccgc gctccaggcc gctccagccg aacgccagaa cctgctcgct tatcttagcc
    15661 ggctttcggg aggccccggc cccgcgcggc agcccgtgtc cgagccaacc ggagccgttc
    15721 ctttcgccga tctgctcaac ccgaagcccg gagagtggcc aacctatcac gggcggctga
    15781 gcggcaaccg ccacagcccg ctgcggcaga ttgacgtttc gaatgtaagc ggcctggcgc
    15841 cccgatggat atttcaaata cccaatgcgg gccggctgga aaccactccc gtggttgtaa
    15901 acggggtgat gtatgtgacc gcagtgaacg aggtctacgc gctcgacgcc ggcacgggcc
    15961 gccagatctg gcattatgcc cggccccgtt cgcgaggcct ggtgggggat gccgcgggcg
    16021 gcatcaatcg cggcgtcgcc gttctgggcg atcgggtctt cctggtgacg gataacgcgc
    16081 acctggttgc tctgcatcgc gttacaggcg ggctgctgtg ggacgtggaa atggccgact
    16141 cgcggcagaa ctacggcgcc acctcggcgc ccctcgccgt caaggacctg gtgatttccg
    16201 gcgtttctgg aggcgacgaa ggggttcgcg gctttatcgc cgcgtacaag gcggcgaccg
    16261 gagagcgcgt ctggcggttc tggacgattc cggcgcccgg cgagcccctc gccaaaactt
    16321 gggccggcaa agcgctggaa cacggctgcg gcacgacctg gctgacgggc acttacgacc
    16381 cggagacgga tttgctcttt tggggagcgg ggaatccctg tcccgactac aacggggacg
    16441 agcggcaggg cgacaatctc tactcgagct cggtgctggc gctgcgccct gagaccggca
    16501 agctggaatg gtattaccaa tatactccgc acgacgttca tgactgggac gcggagcaga
    16561 ccccaatggt cgtggacgcc gagtttcagg gccgccgccg gcggctgctg ctccaggcca
    16621 accggaacgg ctttttttac gtccttgacc ggactgacgg caagctgctg cgggcgactc
    16681 cgtttgtcga gaaactgacg tgggccagcg gaatcgggcc ggacggcagg ccgcgcctgc
    16741 tgcccggagc cgagcctacc ccggaaggcg tgccggcatg cccggccgtc gagggggcca
    16801 cgaactggat gtccagcgcc tacaacccgg ataccggcct cttctatttg atggcgcttg
    16861 agaagtgcaa tatctatcgc aaatcgcccg aggtctggga gccgggcaaa tcgttctatg
    16921 gcggcagcac aaaggatatt ccgggcgagc ctgggaagaa atatttgcgc gccatctatc
    16981 tgcaaagcgg caaaatcgcc tgggagtatc cccagatcgg cccggccact acgtggggcg
    17041 gcgtgcttgc aacggccgga ggtctcgtct ttttctgtga cgacagcggc gcgttcgccg
    17101 ccgcagacgc aaaaaccggg aagctgctgt ggcattttcc tacgagccaa tcctggcgag
    17161 cctcgcccat gacgtatatg gccgatggaa agcagttcgt agcggtcgcc gccggcagcg
    17221 taattctatc tttcgggctg ccataacggc gccggcctct gcgctttttg gaagaccgca
    17281 gcccggcggg cccgagagcg gttgtcgttc tagtatttcg acagcaccgg cttgaagcgc
    17341 tccatgagcg tcttgagctc gttctcttct tccggccgga cgggtgtcag aggcggacgc
    17401 acgggcccgg ccggcattcc taaaatctgc atggcggcct tgatcacgct gacctcatag
    17461 ccccgcttcc gggcgcgaat gttgtacagc gggatcacgt aattgtgcat aagccggtaa
    17521 acgctggccg agtcgaacgc cgtcgcgcgc tcgtgcaatt gcaaacacag cctgggggcg
    17581 atattggaga tagacgacgt gtagcagcga atgcccagag aatagtagcc gggcaccaga
    17641 tcgtcgccga cgccgccgat ccactccagc ctctcgccga cgcgcgcccg gatgcgctgc
    17701 aaaaggcgga tgtcgccttg gccgtcttta tacgccacca tgttcggaat cttgctcagg
    17761 cggtccacca tggcggaaga aaaattggcc caatcgcggc tgtaaataat gcagccgagc
    17821 ctggtggcgg ccgctatggc tgaataatat tcaaagatcc cgtcgtcgtg ggcgtttacg
    17881 tagtaaggcg gaaagatcag aatgccggcg gcgccgaccg attcggcttc gcgggtcatt
    17941 tcgatcgcca gttgcgtgtt gatgcccacg ccggcgatga ccggcagctt gtcgccggcc
    18001 tccgccacgc agaactccac gaccgcgcgg tgctcggcgg gcgtgagcga atagagctcg
    18061 cccgtcccgc cggcggccac cagggctgaa aaagaatgtt ttacaagaaa ctgcacgttg
    18121 cgccgcaagc cttccaaatc gagcgacaaa tccttctgaa acggggttac gggaaacgct
    18181 atcactcctc tgagccggtt cttcagttct tgttggtcca tagccagtca attttgagag
    18241 gggcgagaat cgattgcgcg ctggtggctg cgccgatttc gaccggcatc cttacgaaat
    18301 gcagccagaa tgtcatgccc ttttcgccgc tgccgcggaa cgaccgccag atttgcagcc
    18361 gcccgtcgga tgagacttca atccggtctt cgcgctcgcc aaaatccttg accgccacca
    18421 ccgtgcaccg ctcgcggtcc atcacgtgcg cccagccttc ggcgcgcccc gatttggccg
    18481 ttacataagg ctcgtttccg accagcacgt gccaggattg cggctggccg atcagcgcgg
    18541 cgctctgacc cgggcgcagt tgggtataaa ccaaagtccc cgcgccgaaa tcgaccaagg
    18601 ccggcccgct cgtaaggttc aggttcagat cggcgccgag cccggtgagc aacgcgtttg
    18661 gatcctcgac cgtccaggtt gttttcaccc acgacttgga cctgggaaac tccatgcgca
    18721 ctttcgattg cacgctgtag tttccgcgca gagcttcggt gctcgaaaac tcgattaccg
    18781 aatcgagcgg accctgcttc acgacactgg cgctggttgc cttcccataa ggaccgaagc
    18841 cgcccgcgcg atagatgata tcgtcacgaa accgcagcaa aaggccctgc gaaccgggcc
    18901 gcagatagtc ccagcgcggc gtcttgaccg cgctcagcaa gccgagcagg ttgtctggca
    18961 cgatgtaatg caaggactcg ccgtagctta ctgtaaattg atcgccttcc ctgctgacaa
    19021 tggaaccggc cgcttcgggc gcgtgcattt cctgcggaac caccgagaat tcctgacgct
    19081 cataagggcc cagattgacg ttgaggtcga tttcgccgcc ccgaacctgg gccggcacgg
    19141 gcttgccgtc gcggagcagc cgcacagtgc ggccggccaa ttgcggcggg agagccgccg
    19201 tgaccgggaa gctgaaacgc cggattcctg cggtctctgt gatgcgcaac tgctggccgg
    19261 atagaggcaa gccgagaaga gcggcggcca gcgccgccag cgagggagcg gtgattgttt
    19321 tcttcaaccc tggcgttcgc atgaggcccg cttcacatca gcgtcaagac cacgacggta
    19381 tcaatcgggt cgcgctgaga atgatccacc tttagcatta cgccgagacg attttcgatg
    19441 taatcgacct tgtttccgct cagcagcgcg gcggcgctga tcttgctcgg caatttcggc
    19501 aaagcgagca catcgtcttc caggttgagc aggtgcacat aaaccttctt gcccttctgc
    19561 gtcgtcacgc cccagtcacg cggaggtatc gggccgccgc gagtgccata aatgctatcc
    19621 ccgttcacct tcatccattc gccgacctgc tgcaaccgct cgacgaactc cggctggatc
    19681 gtgccatctg gacgcgggcc gatattcaaa aggaaatttg cattgtggcc ggcggcccgc
    19741 acaaggtaat ggatcagatc gcgggcgctc ttgaactgca agtccccctt gttgtaaccc
    19801 caggccttgt tgatggtctc gcaggtttcc agcgggagcg agccgacttt ggaatcgcgg
    19861 ttgaactcgg cggtcttgcc tcccggcaga tctttttcga acatctggaa gtcttcgcct
    19921 tcgaacggca gatgatgatg gttgttgccg atgagcgccg ccggctggag gctgtgaatc
    19981 atgccgtagg ttttttccag gcgccagtcg gcgttcggct tgtcccacca gccgtcaaac
    20041 cagatgccgg cgatctcgcc gtagttggtc agcagctcgc ggagctgccc atccatgtag
    20101 tccaaatact tataccagtc gccggactcg gggcgccctg tgttccatcc cgtgcgcccg
    20161 cgcggaaagt aatcgggatg gtgccagtcg agctccgaat ggtagaagaa gagcttaatg
    20221 ccttgcctgt ggcactcgtc ggccagcatc ttgagaacat cttttttata cggagtccga
    20281 tccacgatat cccagtcggt cagcttcgag tcaaacattg caaagccgtc atggtgcttg
    20341 ctggtaatcg tgatatagcg catgccggcc gccttggcca gcgccaccca cgcggcgggg
    20401 tcgaatttga ccggattgaa ctgcggcgcc agcttttcat actcctcgac ggtcattttc
    20461 cggttgttca tcacccattc cccgtctccc agaacgctgt aaacgcccca gtggatgaac
    20521 attccgaact tggcgtcctg aaaccactct cgcgccgcga ggttagccgc cgtaggctta
    20581 tactcggcgc gcgcgccgct cgtaagccac gcgcccagca aaaggaaact gcaaattctc
    20641 aggcaagttc tcataaccgc cgtcctcaaa ctgcttcgtt gcaaccggat ttcccccaag
    20701 aatagcaaag gcggcggcaa aacggcgttt ccccgaaatc ggcagcgcgt tcagcaatcc
    20761 ggcgcgcctc ggcagaggag gaaggagatg ttccatctct tttcgcccgt gccggcggcc
    20821 ggcgccctgc cgcctatatc gtccggagct aaaatgagag cggtaaccgg atgccgcgct
    20881 ccgttgagtt cttcgagcac caatttcaac gccaggtcca gatgtgcgag ttcgagttga
    20941 acccgttcga gcggctcgcg ctggattacc tccagggcgc cgtgttggat ctgggttgcg
    21001 gccttggcaa tttgaccctg gcagccgcgc gccgcggctg catcgtaacg gccatcgacg
    21061 cgagccccac ggcgatcgcc aggattcgcg acgctgcgga acgagagcgc ctgccggtga
    21121 gtgcgctgca agccgatttg gccgatttcc agatccaaca cgattacgac gccgtcgtcg
    21181 cgatcgggct gttgatgttc ttccccaagc cgcaggcgct ccgcatgctt gctgaaataa
    21241 aacagcacgt ccggaccggc ggatacgcga tcatcaatgt gctcatcgag ggcacgacgt
    21301 atatggacat gttcactccc ggagagtatt gcctgttccg ccgcgaggaa ctggctgagc
    21361 gcttttcgga ctggacaatc gaggcggctc gctatgacga attccccgcg cccggcgaaa
    21421 ctctcaaggt ctttgcaacg gtgatcacgc gtaatccggc ggtgagttcc gcgcaccggc
    21481 cgcaatgacg gcggacggcg agtccgcaat tatcattttc cgcagtgagt gatcgccacc
    21541 gcgccgcggg gaccggggat gacggctttg ctccacccgc gcgatggccc tcgcgagcgc
    21601 ggagggatcc cggagcagtg cccgttgcgg ccccgcgctt gcctacccta tattgaaacg
    21661 agcggagacc gattatgatt gttcgctgtt tgatcgccgc gctggcggcc ggcggcatcg
    21721 ccgcggcgac tagcccttct cccgttactt tcagccgcga tgtcgcgccg attttgcaga
    21781 agcactgcca gacgtgccac cggccgggag aagccgcccc catgtcgttc ttgacttatc
    21841 gggagacccg cccctgggct aaggcgattc gcgaatccgt actgctcagg aagatgccgc
    21901 cctggtttgc cgatccgcac ttcggaaaat tcgccaacga ccgctccctt tcgcaatccg
    21961 acatcaacac tctggcggcc tgggccgaaa acggcgcccc ggagggcgac ccgaaagacc
    22021 ttcccccacc ccggaagttt atcgaaggat ggaatatcgg ccagcctgac gcgattgtcg
    22081 aaatgcccag cgagttccat gtgcccgcat ccgggacgat tgattaccag tatatagtga
    22141 tcccagccgg cttcaccgag gacaagtggg tccagatggc ggaagtccgg cccggaaatc
    22201 gcgcgctcgt acatcatatg atcgctttcg tgcggcctcc cggctcgaaa tggttgcgcg
    22261 aggcgaagcc gggcgttcct tttgttccta agaagcaaag cgaccggcgc gagagccgcc
    22321 gcgatggcga tgagaccgag ggcgaatttc tggtcggctt cgccccggga agcatgcctg
    22381 aagttctgaa acccggacaa ggcaaactga tcaaggccgg ctccgatttc gtgttccaga
    22441 tgcactatac ggcgaacggc aaggcgggaa ccgatcgcac ccgcatcggc ttgatcttcg
    22501 ccaaggagac gccggccgag cgcgtcctca ctctcgcggc gcaaaatcgc aagtttgcta
    22561 tcccgccgca ggaccccaac tacgaggtgc gtgccggcat cacttttcaa gatacggcgg
    22621 cgctgctcgc catgctgccg catatgcatt tgcgcggaaa ggattttgaa taccgcctcg
    22681 tctatcccga cggccggtcg gaaattctgc tgcgggttcc caaatatagt tttagctggc
    22741 agctttccta ctacctccag gagccgcgca tgctgaccaa gggcgcccgc atcgagtgca
    22801 cggcgcatta cgacaactct cccaataatc cggaaaaccc cgaccccaca aaagagattc
    22861 gatggggcga tcagagctgg gacgagatga tggccggatt ttttgatgtc gccttcgatg
    22921 ctcataagaa tccggagaac ttgcttaaga aggaaaccag gcccgcgcgc gggcacggcg
    22981 aaacagccgg cgggaattga tgtgaggggg cggatcggcg ctcgctgcct gctgttgttc
    23041 tccctgggcg ccggactgtg ccgggcccat gatcccatca caaccaaaat cacctggacc
    23101 cgcgaaattt cgcgcatttt ttaccggcgc tgcttcgcct gccacggcaa agaagggcct
    23161 tccatgccgc tgctcaccta cgaacagacg cgcccgtggg cgaaggcgat caaagaagca
    23221 gtactcgaaa gacgcatgcc gccgtggggc cccgtgaagg gctttggcgg tttcaagaat
    23281 gacgccagtc tctcggaagt ggaaatcgag ttgatcgtga actgggtgga aggcggcgcg
    23341 ccggaaggag aactcatgta tctcgcgccc ccgccggatt tcattcccaa gccgcccgat
    23401 cccccgccgg ccgagcccgt gccaatcaaa ggcttggtaa gactcgatcg cgaggtgacc
    23461 gcaatcggcg tggagccgag cgggtctgtg caagctacag cccatattcc cggcgggggc
    23521 atcgcgccgc tgatctggat ccgcgacttc cggcctggcc agccgaagac gtattacttc
    23581 cgcgaggccc tgaagctgcc caaagggacc gagattcgtt gcgagggcgc tgccgcccgg
    23641 cttctcattg cagcttcgca accggcacag taaacacggc ggtgttcacc ttgccgagcc
    23701 gctgaaactg cacccaaatc cggtacatct gcggacgcgg gaaaatcagg ttgaattgca
    23761 cctgcggccc gccatgggca attgtaggat gttcgtggat cagatcgatc agatcctcgc
    23821 tcgcggccag caggtgtccc cacaccccta gatatggctc caggccttcg gccggcttca
    23881 agcggaaaaa aagcagcgtc ttttggcctg cgatgggcgg ggcgggttcg gttgaaagct
    23941 cgacggcgag attagctgcg tgcttcgaaa cgagatcggg ggccaattgc ggctgcgccc
    24001 cggcgcccgg cgctatgagc gttgcaggca caagctgcgg tgtgccgccc tcaggataaa
    24061 agtcgcacag cagccgatag cggcctggtt taggaagccg cgtttccagg cggaaaacgg
    24121 aatcgccggt aagctgcggg tgctcgtgca gaaaaaactg caaatcctgg ctgatcaaaa
    24181 aaagatgaaa cagcttttcg tgcacagcgc gaaatttctg caccggacgg ccggtaaccg
    24241 gatcgagcac ctgaatctcg aggcgcactt tctggccggc atggaaggcc cgcggaaaaa
    24301 tcgcggtctt gatcggatat tcgaccggat ctggaaggtc cggctcgaga tccatgttgc
    24361 atttggggca ctttcccgga cccttgccgc gaacttccgg atccatcggg cagacccact
    24421 cgaccggcgg ctccggcgcc tgtgcccaaa ggaagggaac caggaaagga gcccccagaa
    24481 tttgtccgag gaatcgcctg cgtctcatct ccggatcaat cggctcctca catcctcaat
    24541 cggcgttctc cgcgccatgc ccgtatctta acaccgggag tccggcccga ctccccgcag
    24601 cggaagcgcg ccagggccga tcgcgctttt ccggcatgca aacgtctccg agatagcgcc
    24661 gcagggctgc gcagtcgtaa atgcgccgga gggactcgcc gatctcaaca gcgcgctcca
    24721 ccggcgggga gtgatccgaa ggacttcgaa agcccgctgg ggcttcgccg caccgaattt
    24781 gataatgtga aaagtccgcc ccggcggaac tcagcttatg cctgcaaaga aactgcgcag
    24841 ccaggagtgg ttcggcggat ccgcctatga agccagcgtc cggcgcgcat ggctgcgctc
    24901 ggaaggcttc ggcgcccagg ttttcggcgg caaaccggtg gtaggcattt gcaattcctg
    24961 gagcgaacta aacaattgca acgcccactt gcggcaggtg gccgaagcag tcaagcgagg
    25021 agtatgggcc gccggcggat ttcctctcga gtttccgacc atctccctgg gcgaactttt
    25081 catgaaaccc accaccatgc tcttccgcaa cctgatggcc atggacgtcg aagagatgat
    25141 ccgcgccaac ccgattgacg gggtggtgct gctctgcggc tgcgacaaaa caacgccggc
    25201 gcaactgatg ggcgcagcct cggcagacat tccggccatt atggtgacgg gcggccctat
    25261 gctggccggc aattggaaag gaaacgtgct gggatcgggc accgatgggc gcaggtattt
    25321 tcaggagctg cgcgccggac gcataaccga cgaacaatgg tgcgaaattg agggctgtat
    25381 cgcgcgcagc gccggccatt gtaccgtcat gggaacggcc tcgaccatgg ccgccctggc
    25441 ggaggctctg ggaatgacgc tgccgggcaa cgcggccatc cccgccgtcg actcgcgccg
    25501 ctaccagctt gccgaagcga gcggagcccg gatcgtcgag atgatccgcc aggatctgcg
    25561 gccctcccgc atcctgacgc gggcggcttt cgagaacgcc atcaagaccg acatggcgat
    25621 tggcggttcc accaacgccg tcgtgcatct ggtggcgata gccgggcggt tgggaatcga
    25681 gctgcccctc tccgagttcg atcgcatctc gcgccagacg ccattcctgg tgaatgtcaa
    25741 accctcaggc gcgtacgtca tggaagattt cttctatgcc ggcgggctgc cggcggtgct
    25801 caaagagctc gcgcctctgc tggacaccac ggtgctcacg gctaccggcc ggacgctgga
    25861 agagaacttg cggagcgtgg aacgctcgac taaccgcgat gtgatccgcc cgctcgatca
    25921 gcctctggcc ccggaaggcg gtacagccat tctctacggc agcctggctc ccggcggcgc
    25981 agtcatcaaa cagacggcgg cctccccgcg cctgctgaga catcgcggcc gcgctgtcgt
    26041 gtttgccagc tacgatgacc tgagggcgcg catcgatgat ccggcgcttg aagttgatgc
    26101 gaactcgata ctggtgctgc aaaacgccgg cccgaaaggc ggccccggct tccccgagtg
    26161 gggccacctg cccattcccg ccaagctgct gaagcagggc gtcgatgaca tggtccggat
    26221 cagcgatgcg cgcatgagcg gcaccagctt cggaaccgac gtgctgcatg tcgcgccgga
    26281 atccgccgtg ggcggcccgt tggccctggt ccggaccggc gacgaaatct ccctcgatgt
    26341 cgaagcccgg cggctcgacc tgtgcgtttc ccaaggggag ctaaaccgcc gccgggcgca
    26401 gtggcggccg cccgccccgc actatacgcg cggctacggc aagttgtttc tcgatcatgt
    26461 gacccaggcc gaccagggct gcgacttcga ttttctccgg gcctgcgggt gagcccgtat
    26521 tcggccgggc caactatgga atcggcaagc caaagtggac actcaacggc cagggaaccg
    26581 gaatcagcag gaacatcaac aaagcgccga gaagcgccat gttcttgagg aagtttaccc
    26641 gctcggcggc ccgctgctgg gggtcctgta tagtccagaa cccgtggatg acaaacgaca
    26701 cgccgacaag aaagatcccc agcaaaacga tccccaaaac aggatacgcc cccagcagga
    26761 tgctggcgcc tcccgcgatg agcaatacgc ctgtggcaat caccgcaagc cacggcgcag
    26821 gagtgccttt actccgcgca taggcgctca tgttatggac atcggcaaaa tggtgaaagc
    26881 cgctggccaa aaagaagacg ccaaagagaa ttctcccgat cagaaacagc gccacagccg
    26941 tcatgggcaa gccctctcgt gtccaattgg atcggaagat ttgattttgg ttgcaaaaac
    27001 agaagcgcgc acatggcggc tcggacgggg ggagaaaaac agcccgcaaa gccttaatcg
    27061 acgcccagcg aagccggctt tgtctcttcc atgggaacac gcgcgggagc gctcccagga
    27121 acagcgcccc gcgcgccagc cgccgcactg ccggccaaag ctcaggcatc ggtcgtctcg
    27181 cctgcccggc aacagcaaga aatactttct attttcttct taagttactt tttaaaaagg
    27241 atatattaca aattcaatca aagcctgcgc cgccgtgaca gcacaagatc cgcggtcgca
    27301 caggcggagg cgttccaccg gacggaagtg gaccgttaac gtccggcggc ttaccggcgg
    27361 tagaggtaga gcgtatgggt gcggatgtcg ccctgatcgt cgtcctcgat attgatggtc
    27421 ttttccgcct tccagccgcg gaactcgaag tcgtgcgaga ccacacgcga gcctttatgc
    27481 atgctttttt cgagaagtgg ctggatcttg tcgttggagg tcggcaacag gtatacggta
    27541 atcaggtccg gcttggagaa atcagccgtc atgagatcct gcttccggat ggtcgctagt
    27601 ttttcgaggc cgagatcttt aatcttcgcc atacttcgct gataaagaga atcatcgatc
    27661 tcaaacccta ccgaatgcgc tccgaacttc tgcgcggcca tgatgacaac ccggccgtcg
    27721 ccgctgccca gatcgtagtg ccactcaccc tttttcaact ctccgagctc cagcatcttc
    27781 tggactacgg cttccggcgt ggggtaaaac ggcgccagct tctcgacttc ctgctgctgc
    27841 gcccggccgg ccacaatggc aatcaggatc gctccgccgg ccaccagcat tgacttgcgc
    27901 atacctgctt ccctcctgtc ccaagccccc cggccactgc aagcgaacgc ttccttgtgc
    27961 gaagggcctg gctgcgttag atgcgtcccc aggatggtaa catgggcgaa accgccatgg
    28021 aacttttcac cgtaaagatc accattcccg aagctgccaa cgtcatagta ggccagacgc
    28081 atttcatcaa gaccgtggaa gatctttatg aagcgatcgt gaccactgcg cccgggatga
    28141 aattcggcgt cgcctttaat gaagcgtctg gcgcctgcct gacacgggtg gacggcaacg
    28201 acgaggaact acaacgggcc gcggcggaga acgccctctc gatcggggcg ggccacgttt
    28261 ttgtgattat gctgcgggac ggctacccaa tcaacgttct aaaccgcatc aaggacgtta
    28321 gcgaagtgtg cgcgatctac tgcgcgacag cgaatcccct ggaggtgatc gttgcccaga
    28381 ccgggcaggg ccggggcatc ctcggcgtcg tggacggttc gccgccgaaa ggcatagaag
    28441 gcccgtctga cgctgcctgg cgcagggatc tgctgcgaaa aatcggttac aaacgctaag
    28501 gggcgtgttc tggcccccag gaacgcagcg ctccagcgtt ttccggcggc gaagccgttg
    28561 cagagccggc tgcgttcagg cgctgcaacc acgcgatgat ctgccagcgc tgggcttcgg
    28621 gaagcctcga gaaggaaggc attcctgagg ccagcgagcc gttgcgcagc acccaaaaca
    28681 gcgcgcccgg catcgcgccg ccgacctccg gggagacgag cggcggcgct ttgccattcc
    28741 cgcgggcatc ctctccgtgg caggcggcgc aatagcgccg gaacagcttc gcgcccgccc
    28801 tttcagcctc ccgattccct tgatacggat tgggttcgga acgcgagacc gctccggctc
    28861 gctcaagggg cgagcgcgaa tggcggctca agacggctgt ttgagccaat gtgactccaa
    28921 cggcagcaga aatcagcaac cccggccaaa ttttattttt cataacttag cggaacattc
    28981 ggctgaccac gcggccgaat tgcgcgactt cgtaggacac gccaaaacgc aggagaaagg
    29041 gaacgctcga tccagtcagc ccaaaggccg gcgagatctg caacgaggcg ccgtttggca
    29101 gttgccaggc gacaaccggg gcgaggtagt gggccatgtc ggctacggaa ccgaaatgct
    29161 catgtgtacc cagtcctccg tatagttcga
//
LOCUS       DASREK010000220        21299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_110450, whole genome shotgun sequence.
ACCESSION   DASREK010000220 DASREK010000000
VERSION     DASREK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 21299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21299
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110450"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1730
                     /locus_tag="VFA54_11395"
     CDS             105..1730
                     /locus_tag="VFA54_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51459.1"
                     /translation="MSSRATAWRERLNGFSRRDLFRGGGWLAALGLLPKRSAGGAARP
                     APALSVGRNLYQSIGVRPLINCKGTFTIISGSQTLPEVKAAMEEASRHYVHLDELMEA
                     VGARLAELTQAPWGIVTAGCAAALTHATAACIAGADPEKMQRLPNVHGLKSEVIMPRS
                     SRNVYDHAIRMLGVRIVEVDTKEQYLAAINDNTAMVCILGGPSDKFLDEVAEATKPKG
                     VPILVDAAAEELTIPNVHLKRGATMVAYSGGKCIRGPQCAGLLLGPKDLLQAAWLNSA
                     PHHAFGRSLKVGKEEIMGMLAAVEMWVKRDHQAEWKTWESWLANISSKVASIPSVRTE
                     VLQPSEGLSNHAPRLRISWDAERVGIAGNELEKYLYDNEPRIVLGGSTGAWGSKQQSS
                     VSIMPYMMMPGDDKIVAARLYEVLSKPPQIRRAPEEKGQPAAVAGQWNIRLDFVRGSA
                     NHVLFFEQQGENITGTHRGSVLAGDLRGTVKGNQVRFRSSFPYEGTRLNYQFEGRLEN
                     GALAGVVDLGEYGTARWTGQRHQYGNGELRRGA"
     gene            2029..2814
                     /locus_tag="VFA54_11400"
     CDS             2029..2814
                     /locus_tag="VFA54_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51460.1"
                     /translation="MYNSLSGDAGEEIPVRSAEMVRMRRLATALCVCSMVGGAQDPLA
                     WFPLQVGSRWVYEHEWKSGDRSRPDVDRWITEETVTGWVTIPEGLVVLRQVQQQDNST
                     GQTIKGRVLPRNSQLRAVQQPSNAHPGYLVTRDHEPYLVHGNCIYVIADGWDGPKQQL
                     RPTYREYLAKGAIAPDFCFPLQTGRQWGNTDIPWRVERAGEGVGLFLLPEYAGAIHIF
                     SNHFGSGGQEDVWFQKGVGVVGEHYIHNGTYDEYTKKLLSFSR"
     gene            2981..3793
                     /locus_tag="VFA54_11405"
     CDS             2981..3793
                     /locus_tag="VFA54_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS51461.1"
                     /translation="MDATIPDVDGIFAEACRLAGIERYDLSAEDLVRAIEESVKRYAP
                     AADGRELRKFILSLRLEDLALATACARGSGRAWEEFYKKYHQYLIDTAGEYDLADQVI
                     AELYGMDGQGKIGNFRGRSTLKTWLHALVHQAQVDRHRREARLVGFDSLPAEPAKQEH
                     PESFEGRDAAAALARVLRREIAKLEAPQRLLLAWYYSDGLRLAQIARMRGAHESTVSR
                     ELEGIRKRLRKQVLQRLQDEGFSRARIEESFRHLAEAPVDFDGIMDKARNTR"
     gene            3808..4980
                     /locus_tag="VFA54_11410"
     CDS             3808..4980
                     /locus_tag="VFA54_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZS51462.1"
                     /translation="MARPAESASNRLLEQVLRKLLQASGQPSPASTETPSGSHPEQEL
                     VAAFAEGRLRGRELQSVESHLAACLLCRRSATALFESLGNVERPRSQAIFEWRRWMRP
                     APVLAAALIVGSMLYYQRGQILRRGVQPARSNAQNGLAPASGQTEAAGQKTQEAGAAV
                     RRDSNAAQNSPGLRNGNQAKAVERVERTAPGNGGVIAQVAGAAAVAPQGARAASGGAP
                     QDGNTVLLKEEAEQAAARAQQSPIQNKPLSGKAEENRGPDQAPVPRAEAPLAREGSEK
                     GQPPALKKTFPAPAASPARSRAFSAGVVGGIAGGWLEPVAALPGGAPLRAMIAHDNRT
                     WAVSDSGKIFRSLDSGRTWIAIPSPTAEDLVSIRWDPATNSLMVRDKQGREYQAQP"
     gene            complement(5079..5489)
                     /locus_tag="VFA54_11415"
     CDS             complement(5079..5489)
                     /locus_tag="VFA54_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51463.1"
                     /translation="MELSTVEKNNSNTKRILLFDLDRAQGSELERVLTSQNLVVHSER
                     SLPLTQCLRLVEEYRADLIFCPAQRAHYKKLLDALGERRPGLPVIVVSPYPEVAEWLD
                     AIEAGASDYCTPPFDAAQIAWILETALKSRSVAA"
     gene            5740..6465
                     /locus_tag="VFA54_11420"
     CDS             5740..6465
                     /locus_tag="VFA54_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HZS51464.1"
                     /translation="MDAPILSNENPEVDFLHEIGTRMASDPLPVVLNRVVQFVSALIK
                     CDSCFLYVLEGDELVLRASKNPHPEEVGKVRLRIGQGITGWVAEHLQPVAVARDASQD
                     PRFRFFGELPEDRYEAFLSVPVLCRGRVVGVINLQHRLPHTHTRREIQLISTIGFLVG
                     AEIEMARLEGEISHLSDQLETRKLVERAKGILQRELGINEEEAYLALQRQSRQRRKSM
                     KEVAQAIILSEEVKRESQQPPGE"
     gene            6546..7160
                     /locus_tag="VFA54_11425"
     CDS             6546..7160
                     /locus_tag="VFA54_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51465.1"
                     /translation="MTRNGFDRREAAVLRKLSSPAKIQQFLDAEIGYNKEKDGDTCRS
                     PRRVLRDRVAHCMEGALFAAAALRVLGFPPLIVDLESVRDDDHIVAVFQQHGCWGAIA
                     KSNYAGLRFREPVYRTLRELAMSYFEHYYNPAGEKTLRAYSRPVNLSRFDSIGWMTAE
                     EDLWAIPEYLYTIPHRRLLTAEMERRLNRMDRRLFAAGRVGAVE"
     gene            complement(7240..8589)
                     /locus_tag="VFA54_11430"
     CDS             complement(7240..8589)
                     /locus_tag="VFA54_11430"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZS51466.1"
                     /translation="MSEFALLSRSRCQEIFDRVKKFSSTDDFEILLIASRQALTRFAN
                     NVIHQNMEEETQVLSVRVVIGQRTARATTNRLDDDSIRRVVEEAAAIAKFQTPDPDLP
                     PMAEPFTYKTVERFDEPTAETSAHWRAGEVKEAVDRASANSMTAAGVFATGASAEAIL
                     NSRGLFAYHAQTRAEASITAIAPDSSGWAKSNSVRVQEIDPPALASRAVEIASASRHP
                     VKMEPGAYTVVLERAAVLDLLGFLAFDFSATAVRDKRSFLTDRIGKQLFGANITICDD
                     VYHPLQSGAPFDGEGVPRQPLVLVDRGIVRNVAYSRRAAKAAGVEPTGHGFPLPTEYG
                     EAPVNIVFSGGDQSVDQLIHSVDRGVLVTRLWYIREVDPYEKILTGMTRDGTFLIEGG
                     KIVHGVRNFRFNQSMIELLNNVELMSPAERTAGEEAFEMVVPALKARSFHFTAVTKF"
     gene            complement(8703..10157)
                     /locus_tag="VFA54_11435"
     CDS             complement(8703..10157)
                     /locus_tag="VFA54_11435"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZS51467.1"
                     /translation="MQDFALLALDRAVQAGASYADVRLVESAERLVSTKNGRVGQASS
                     SESLGLGVRVIASGAWGFSATDWLTAEGVQAAATRAVEIARASALVKKRDVALAPEQK
                     HEAQWSSPVQIDPFSVPVDQTLDLLLKVDAELRRVQGVTLAEAAVTCRRLRQFFASSE
                     GSRIEQTRWYTGAGCVAYSYSGNEIQKRSYPNSFGGQHMLKGYELVHELRLLENAPRV
                     AEEAVALHRADQCPEGVFDLILDSSQLALQIHESIGHPIELDRVLGAEANFAGMSFLT
                     LDKRGKLQYASEIVNVVCDARIEHGPGLGTFAFDDEGVPAQATDIIRDGRFVGYMTSR
                     ETAAEIGEPRSNGTMRADNWNHIPLIRMTNVSLKPGKMTPEELIESTDRGIYMETNRS
                     WSIDDKRYQFQFGTEIGWEIKNGKKVRMLKNPSYSGITTEFWNSCDAICGPEYWTLWG
                     TPNCGKGQPQQTMGTGHGASPSRFRNVKIGAAYA"
     gene            complement(10238..11515)
                     /gene="ahcY"
                     /locus_tag="VFA54_11440"
     CDS             complement(10238..11515)
                     /gene="ahcY"
                     /locus_tag="VFA54_11440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZS51468.1"
                     /translation="MSTVNTSVRHDVKDIGLADLGKRRIEWAFQSMPVVQSIRKQFIK
                     TQPLAGIRISACLHVTTETANLMIALRDGGASVVLCASNPLSTQDDVAASLVRDSGIS
                     VYAIKGEDNATYYSHITAALEHRPQITMDDGADLVNMLHTARQDMASEVLGGTEETTT
                     GVIRLRAMAKQGVLKFPIVAVNDAMTKHLFDNRYGTGQSTIDGILRATNILFAGLNVV
                     VAGYGWCGRGLASRARGLGAQVIVTEVDPIRALEALMDGYRVMSMLDAAPIGDVFITV
                     TGDKGIIGAEHFERMKDGAILCNAGHFNVELDLECLSKIARSRRPVREFVEEFTLRGG
                     KKLFVLGEGRLINLAAAEGHPASVMDMSFANQAMALEYMVKNHSKLEKKVYPVPEELD
                     RKVAQMKLESMGIKIDRLTPEQEKYLSSWSEGT"
     gene            complement(11572..12732)
                     /gene="metK"
                     /locus_tag="VFA54_11445"
     CDS             complement(11572..12732)
                     /gene="metK"
                     /locus_tag="VFA54_11445"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZS51469.1"
                     /translation="MFHRRLFTSESVTEGHPDKIADQVSDAILDAVLAQDPLGRVACE
                     TLVTTGLCFIAGEITTTARVNYTEVARETIRGVGYTRAKYGFDSDSCAVINAIHGQSP
                     DIALGVDRDGAGDQGMMFGFACDETEELMPLPIMLAHKLVRRLSDVRRFGTLKYLRPD
                     GKSQVTVEYEGHRAVRVDAVVVSTQHSADIDQSQLQADVVEHVIRPVIPREMVDSGTK
                     IHINPTGRFEIGGPAGDTGLTGRKIIVDTYGGMGRHGGGAFSGKDPTKVDRSACYMAR
                     YIAKNIVASGLAKRCEVQLAYAIGVADPVSVLVDTQGTGQVADVHLSELVRELFPLTP
                     KGIIDYLQLRRPIYKKTAAFGHFGRNEDGFTWERTDKAEEFRAQAAALMARV"
     gene            complement(13187..13900)
                     /locus_tag="VFA54_11450"
     CDS             complement(13187..13900)
                     /locus_tag="VFA54_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZS51470.1"
                     /translation="MSCEGAQTARLIESFPIAPDVRHFLFEAPALERVVFQPGQFVCF
                     HYETGGQIIRRAYSIASPPNGDNLFELCLKRIAGGPLSSYLFQMRSGDEVRFEGPHGG
                     FQLRQPVRNCFFVAAGTGVAPIRSMIGYLLRHGVPPHLQLLLGSRSEECILYRSEFEQ
                     FAARHANFRFYPTLTQGGPEWRGLRGRVQEHVIRLAGDGPHTDFYVCGPGSMVKEVRD
                     ALERNGADPRSIFYEKYAA"
     gene            complement(13897..14295)
                     /locus_tag="VFA54_11455"
     CDS             complement(13897..14295)
                     /locus_tag="VFA54_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HZS51471.1"
                     /translation="MPPGNTRRPEKAAVIFFAHGSSVESANQAVREVSRAAAGKAGFD
                     LWSAAFLEGGQPNLAAAVAQLAAAGARRVIVVPYFLTFGLHLQRDFPCLVAEVAAAHK
                     DIDIVCGDPLDGHPGLVEILAERARQAYER"
     gene            complement(14282..14713)
                     /locus_tag="VFA54_11460"
     CDS             complement(14282..14713)
                     /locus_tag="VFA54_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZS51472.1"
                     /translation="MAQISQILSDRELYFVEPHQTVFEVATKMADLHVGAILVLKDGE
                     LCGIFSERDLLNRVLVEGRDPRLVRVAEVMTPSPTTVDESATADEAMEVMHRHNCRHL
                     PVLRNGRVIQMISMRDLMYYDLERKAEEIQHMREYINGAAR"
     gene            complement(14889..15569)
                     /locus_tag="VFA54_11465"
     CDS             complement(14889..15569)
                     /locus_tag="VFA54_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51473.1"
                     /translation="MTCAICHRRKAKRDCPAVRSAICPQCCGREREETLDCPLDCPYL
                     MESREHEKRPGLDPENFPYKEIRISDTYLRQNEELLTALGEFVLAAALNTPGAADRDV
                     RDALDALARTYKTLESGVYYETEPRSPVAQAIARYIQEALRAFRKEETQRTGMTRTRD
                     ADVLGILVFLLRMALDRDNGRKRGRSFIDFLRRHFGPADSSGRIELAGGRAVGSRASG
                     EPSLIVPG"
     gene            15589..17232
                     /locus_tag="VFA54_11470"
     CDS             15589..17232
                     /locus_tag="VFA54_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51474.1"
                     /translation="MTLGSQRRYAIAAYLILASVYLLYLPHAEFVLDDWLVLRRYQEV
                     RQAGAAAQIKMLVAIIENQFHGQFRFQWLSFSFAYLLFLAVGYSPKVIFAVFLLLHVA
                     SALVLRDVLERLKIGAGPAFLGGAVFLLLPSTHGPVFWSHNCSYYIWSTFWFLLYMRS
                     LAASFAAGRLTAPAAGRQALFLTLALFSGDPIFCLLLAGAPLAAWYLHSKAGRKATLL
                     AWSTVGIMGALYALLINRARIIQAGVGLRYSFTHANLRSNVHSILETYGRLTGFATGS
                     FYAIQPTFTALAFAALAASLVIFGLKMCEARRAPIRRTLLLAAGLWAAAYGPIWFLLA
                     HEFRYDYVPSPYLALGIAALVCWSPALRLPLAGALMAWLAAANVADIRQCWIPQSDNL
                     RLVARSLRATDPRPGDLIIVSQTPMWIGTAPNFAFLASWASTPFAEHVTGVHGLETGT
                     DIIDEGGRLRVFHYDHLEDLTPERAAHTVLLVEEGNGRIGRRHLLADEARPGGYRLYA
                     LKGYTGPEVPSVDVTRERLSFLEGDIYFARRYAHHKYEH"
     gene            17222..18835
                     /locus_tag="VFA54_11475"
     CDS             17222..18835
                     /locus_tag="VFA54_11475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS51475.1"
                     /translation="MNTERQWRAVLLAALLVAFIGRIVATYHVFNDTFDESDHITAGL
                     EILERGRYTIEAQHPPLSRLVLAVLPYYFAGLRSGHYNDLWGHGPWASRDLAFYWKSL
                     SLARTGNLVFALMLFCFVYLWAACLYGPWGGVAACALAVCCPNLIAHAGLATVDMAAA
                     ATILMSAYFFWRWCEQPSLAYCVLSAVALALAVLSKLSTLLFLPPLGLLYFLLGRGSR
                     LSSVTGRGFRMALRRAAVFGLLVFMIVWAGYLFEVGTLVPPGHRYQSPFGMHGGSGLA
                     ATLVRSLGTRKLPAFRLLQGVLEVATHNSSGQRCYLLGRIYWHGSWLYFPVAILVKST
                     LPMLLLAAGGAALFIFGCGLVSRRTIYPLSAAALILMLSMPAAINIGVRHVLAIYPFF
                     AILGSALFTTGRRWITLAALALCAWHAGESIAAHPDYLAYFNEIARGREEQFLIDSNL
                     DWGQDLARLGQYVRARRIDSIYLTYFGRTDPAKMGIAAIQLGDQRPDQGCAAVSVTRL
                     IMDSNLAWLRERKPMGRVGKSIWLYDFSR"
     gene            complement(18908..19852)
                     /locus_tag="VFA54_11480"
     CDS             complement(18908..19852)
                     /locus_tag="VFA54_11480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS51476.1"
                     /translation="MPSVSIVAPVLNERENIQELVKRLKQALGADLQEIIFVDDGSTD
                     GTAELLAAMHEQDPRVKALVFTRNFGHQAALSAGLDFACGEAVVLMDGDLQHPPELVP
                     AMVELWRNGAAIVSTIRLVQRDASRLKRLGTAFFYRLINLISDTPIDPNSADFRLLSR
                     PVVEQLRRLEERQRFLRGLINWTGYRQATLPFEAPPRVAGRTKYSGPKMAALALDAIL
                     SFSRFPLRLAVYIGLLMWVVSFAYAIYALYIKFSVRTAVPGWASIVIVGSLIGGFQSI
                     LLGLVGEYVGRLYEEIKRRPLYLVARTHGLDASPCGQR"
     gene            complement(19845..>21299)
                     /locus_tag="VFA54_11485"
     CDS             complement(19845..>21299)
                     /locus_tag="VFA54_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51477.1"
                     /translation="RVFWVGFLPVLVASLAAGLHGWPYYLLALLAHGITAVLLAGIVS
                     LLAADDRTGFIAGAIYMVFPAANGALFWPVATSFYYVQALAFSAWFYYTWRKLAAAGD
                     YRYGWKDFAILVLTVFTGEQILPLLVGLLPASWWIFGRPKKHRRFFRYCAIHTALLMG
                     LLSIYVLALNRMPVLGGFQHRYHNANFPSLWAIPLRLLAALGLRPSLAEWRPSWRPEI
                     PLLVLLLAAGAAFALGLRRREPNASAPSPLRLLVWSAAGAFLTFLPVVSLPGIEWRYL
                     YVPAIFLGAAAAALTGWAPPQARAILAFLAIAYGISLTYFEMRQCWIPESREAQAIVS
                     TVENAAPIRPGEIFIFAHAPHAMGTAPSFIAGASWSLNSLLLGYAPQVQGARELLVNE
                     QGDLALYRRDSIVLFSRDAISRLRVYVRDAGGHYVRKSLLALPAPDRRYQLLPLQSGV
                     RCPAQPLTIEELQRLALYGDTYFAHFFSGPFPHA"
BASE COUNT         4252 a         6482 c         6426 g         4139 t
ORIGIN      
        1 ttcgcatacc cttattcctg gccgcggcct ggccaacaat cggggcaacg accggttgct
       61 ttcttgccga caaagtttta cgctgatcca ccgaggacca tcctatgtca tcccgagcga
      121 cagcatggcg agaaaggctc aatggattca gccgccggga cctgtttcgc ggcggcgggt
      181 ggctggcggc tctggggctg ctgcccaagc gatccgcagg gggcgcggcg cggcccgcgc
      241 cggcgttgag tgtgggccgc aacctttatc aatccatcgg agttcgcccg ctgatcaatt
      301 gcaaaggaac tttcacgatt atcagcgggt cgcagacgct gccggaagtg aaggcggcca
      361 tggaagaggc ctcgcggcac tatgtacatc tggacgaact gatggaagcc gtcggcgcgc
      421 gcctggctga gctgacccag gcgccctggg gaatcgtcac cgccggatgc gcggcggcgc
      481 tgacccacgc gactgcggcg tgcatagcgg gcgcggatcc cgagaaaatg cagcgcttgc
      541 cgaacgttca tgggctgaag agcgaggtaa ttatgccgcg ctcctcacgg aatgtttacg
      601 atcacgccat ccgcatgctg ggcgttcgta ttgtcgaggt cgataccaag gagcaatacc
      661 tggccgccat taacgacaat accgcgatgg tgtgcatcct gggcggcccg agcgacaagt
      721 tcctcgacga agtggccgag gcgaccaagc cgaaaggcgt gccgatcttg gtggatgcag
      781 cggccgagga gctcactatt cccaacgttc acttgaagcg cggagcgacg atggtcgcct
      841 acagcggggg caagtgcatt cgcgggccgc agtgcgctgg tttgttgcta ggcccgaaag
      901 atctgctcca ggcggcgtgg ctgaatagcg cgccgcacca tgcttttggc cggtcgctga
      961 aagtcggcaa agaggagatc atgggcatgc tcgccgcggt cgaaatgtgg gtgaagcggg
     1021 atcatcaggc cgagtggaaa acctgggagt cctggctggc caatatttca tccaaggtgg
     1081 cttcgattcc gagcgtgcga acggaggtgc ttcagccgtc ggaagggctt tccaaccacg
     1141 ccccgcgcct tcgcatttcc tgggacgccg aacgcgtggg catcgccggc aacgagctcg
     1201 agaaatacct ttacgacaac gagccccgaa tcgttctggg cggttcgacc ggcgcttggg
     1261 gatccaaaca gcaaagctcg gtcagcatca tgccgtatat gatgatgccg ggagatgaca
     1321 agatcgtggc tgcgcggctc tacgaagtgc tttcgaaacc gccgcaaatc cggcgcgcgc
     1381 ccgaagagaa gggccagccg gcggccgtgg cggggcaatg gaacatccgg ctcgatttcg
     1441 tgcgcggctc ggcaaatcac gtgttgttct ttgaacaaca aggcgagaac attaccggca
     1501 cgcatcgcgg cagcgtgctg gcgggcgact tgcgtggaac ggtcaaaggc aaccaggttc
     1561 gttttcgcag ctcttttccg tacgaaggca cgcgcctcaa ctatcaattc gagggccggc
     1621 tggaaaacgg cgcgctcgcc ggggtcgtgg atctgggcga atatgggacc gcccgctgga
     1681 ccggccagcg gcaccagtac gggaacggcg aactccgccg cggagcatag cggcgcattt
     1741 gcctggaatc gcttccgtcg gctcgcgtcg cggcggtttt gaccgccgga cgcgctccgc
     1801 gatttgggat tgctgctgtg gctgaggctg aggcctccgt aattcacatc cttgaacccg
     1861 tacggaccac tcgcccgtgc ttcctggcgc cgtcgagcgc tgtttttggg aacccgctgc
     1921 acggcgccta cggcgggcac gaggcagttg aaacagcaat tcttgacctg aaagataggc
     1981 gctgagccgc acccgccggc gacatcttct atttatgggt cgagtcgcat gtataattcc
     2041 ctcagcggag acgccggaga ggaaattccg gtgaggagcg ccgagatggt ccgaatgcgc
     2101 cgtttagcaa cagccctgtg cgtttgttcg atggtcggtg gcgcccagga cccactggcg
     2161 tggtttcccc ttcaggtggg cagccgctgg gtatacgaac acgagtggaa atcgggtgac
     2221 cggagccggc cagacgtaga ccgctggatc acggaggaaa cggtcaccgg ttgggtgacg
     2281 attccggagg gccttgtggt gctgcggcaa gtgcaacagc aagacaactc taccgggcaa
     2341 accatcaagg gccgcgtgtt gccacggaac agccagttgc gggctgtcca gcaaccaagc
     2401 aacgcccacc ccggctatct ggtgactcgt gaccatgagc cgtatctcgt ccatggaaat
     2461 tgtatatacg tgatcgcgga cggatgggat ggccccaaac agcagcttcg cccgacctac
     2521 cgggagtatc tcgccaaagg cgccattgca cccgatttct gtttcccttt gcagaccggc
     2581 cgccagtggg ggaacactga tattccctgg cgggtcgagc gggctggtga gggtgtgggc
     2641 ttattcctgc ttccggagta tgcgggggcc attcatattt tttccaacca cttcggcagc
     2701 ggcgggcagg aagatgtttg gtttcaaaaa ggcgtcggag tcgtgggtga acattacatc
     2761 cacaacggca cctatgacga gtatacaaag aagcttctgt cgttctcgcg atgacccggt
     2821 tccgggatct gtggcaaaca caacttccgc attatgaacg cagcgcattg ggggggctcc
     2881 ggagttcgca ttttcttccg aaactgcgcg gttccgcgcc gccatgcccg ccctacggag
     2941 gaggcggcat tcggcggcag ctcacggttc gagcgacaga atggatgcta ctatcccgga
     3001 tgtggacggc attttcgccg aggcttgccg gcttgcgggc atcgaacgct atgatctgag
     3061 cgccgaggat ctggtgcggg caattgagga atcggtgaaa cggtacgcgc ccgcagcgga
     3121 cggccgcgag ctgcggaaat tcattctgag tctgcggttg gaagatttgg cgctggcaac
     3181 cgcctgtgcg cgaggcagcg gccgggcctg ggaggagttc tacaaaaaat atcaccaata
     3241 tctgatcgat accgcaggtg aatacgacct ggcggaccag gttatcgccg agctatatgg
     3301 catggacggc cagggcaaga ttggaaattt caggggccgc tcgacgctca agacctggct
     3361 gcatgccctt gtccatcagg cgcaggtgga tcgccaccgc cgggaagcga ggctggtcgg
     3421 ctttgactca ctgcccgctg agccggcgaa acaagagcat ccggagagct tcgaggggcg
     3481 ggacgccgca gcggcgctgg ctcgcgtcct gcgacgagag atcgcgaagc tagaggcgcc
     3541 gcagcgcttg ctgcttgcct ggtactacag cgatggtttg cggctggcgc aaattgcccg
     3601 gatgcggggc gcgcatgaat caactgtcag ccgggagctc gaaggcatcc gtaaaaggtt
     3661 gcggaaacag gtgctccagc ggcttcagga cgagggcttc agccgcgcca gaatcgagga
     3721 aagcttccgg catcttgccg aagctcccgt agactttgac ggcataatgg ataaggcaag
     3781 aaataccagg tgaaacgtac taagtatatg gcgagaccag ccgagtcagc ttctaaccgg
     3841 cttttggaac aggtcctgcg aaagctattg caggcctccg gtcagccaag cccggccagc
     3901 actgaaacgc cgtccggctc gcatcccgag caggagctgg tcgcggcctt cgcagaaggc
     3961 aggctgcggg gccgtgaact ccaaagtgtc gaatcgcacc tggcggcttg tctcctgtgc
     4021 cgccggtcgg cgactgcgct gtttgagagt ctgggaaatg tcgagcggcc acgcagccaa
     4081 gccattttcg aatggcggcg ctggatgcgg cccgcgcctg tgctggcggc cgctctgata
     4141 gtggggtcga tgctgtacta ccagcgcggt caaattcttc gccgcggcgt tcagcccgcc
     4201 cgctcgaacg cgcaaaatgg tcttgcgccg gcatcaggcc aaacggaagc agcgggacaa
     4261 aaaacacaag aggcaggcgc ggctgtgcga agagattcga atgcggcgca gaacagcccg
     4321 gggcttcgaa atggaaatca ggcgaaggcc gtagagcggg ttgagaggac cgcccccgga
     4381 aatggaggcg tgattgcaca ggttgccggc gccgcggccg ttgctcctca aggcgcccgc
     4441 gcagcttcgg gcggcgcccc gcaggatggg aacacggttt tgctaaaaga agaagctgag
     4501 caagccgccg cccgcgcgca gcaaagcccg atccagaaca agcccctttc cggaaaagcc
     4561 gaagaaaatc gcggcccgga tcaggctccc gtgcctcgag cggaggcgcc gctcgcgagg
     4621 gaaggctccg aaaaagggca gcctccagcg ttaaaaaaga cgtttcccgc gccggccgcg
     4681 agtccggccc ggtcccgtgc tttctctgcc ggcgtcgtcg gcggaatcgc cggaggttgg
     4741 ctggaacctg tggccgcttt gccaggagga gcgccgcttc gcgctatgat cgcgcatgac
     4801 aaccgcacat gggcggtttc cgacagcgga aaaatctttc ggtccctgga ctccggcaga
     4861 acttggatag ccatacccag cccgacagca gaagatctgg tcagcatccg ctgggaccct
     4921 gctacgaact ctctcatggt acgcgacaag cagggccgtg aataccaggc ccagccgtag
     4981 accctgtgcg tcgacgcgcc ggtgttagaa ggcgtgatct tgcagagccg ccgcaacggt
     5041 ccacccgccg aagccgccgg gccggataaa tcggcggact aggcggcgac cgagcgggat
     5101 tttaacgctg tttccaaaat ccacgcgatc tgcgctgcgt caaacggagg cgtacagtaa
     5161 tcggaagcgc ccgcctcgat cgcgtccagc cactcggcca cctcgggata ggggctcacc
     5221 acgataacgg gcagtcccgg tcttcgttct cccagtgcat ccagcagctt cttgtaatgc
     5281 gcccgttggg cggggcaaaa gatcagatcg gcgcgatatt cttcgaccaa ccgcaaacat
     5341 tgtgtcagcg gcaaggatct ctcggaatgc acaaccaggt tctgcgaggt gagaacccgt
     5401 tccagttcgc tcccttgcgc gcggtcaagg tcgaacaaaa ggatcctttt agtgtttgaa
     5461 ttattcttct ccaccgtcga taactccata cagcccactt ttagtgattt tctctacgcg
     5521 gagaaaagcg ctggtcacct gggactgtct taacaataaa gctctcaccg gaggaagtcc
     5581 aatcaaaaat agttatgccg gccattagta agccggagag ccaaattttg caaattttca
     5641 tgcggctgca ataaagcgag atggaattcc cgcagcgatt atcgggccga taaaaacatt
     5701 ggattggact gtactccgcg ccagctttaa cttaagaagg tggacgcgcc gattttatcc
     5761 aatgaaaatc ccgaggtaga tttcctgcac gagatcggaa cccgtatggc ttccgatccc
     5821 ctgcctgtcg tattaaaccg cgtggtgcaa ttcgtgtcgg ccctcatcaa atgcgactcg
     5881 tgctttcttt acgtgctgga gggtgacgag ctggtgctgc gcgcctccaa gaatcctcac
     5941 ccggaagaag taggtaaagt gaggctccgg atcggccagg gaataaccgg ctgggtcgcc
     6001 gagcatttgc agccggtagc tgtggcccgg gatgcttcgc aggacccgcg ttttcgcttc
     6061 ttcggcgagc ttccggagga tcgctacgag gcgtttctct cggtgccggt gctgtgccgg
     6121 gggcgcgtgg taggagtcat caatctccag caccggctgc cgcacacgca cacccgccgt
     6181 gaaattcagt tgatttcgac gatcggcttt ctggtgggag cggaaattga gatggcgcgt
     6241 ctggaaggcg aaatttcgca cctttccgat caactggaaa cgcgcaagct cgtcgagcgg
     6301 gccaaaggca tcctccagcg ggagctcggc atcaacgaag aggaggccta cctcgcttta
     6361 cagcggcaga gccggcaaag gcgcaagtca atgaaagaag tggctcaagc cattatcctc
     6421 agcgaagaag tcaaaaggga aagccagcag ccgcccggcg aataggccat cgccctgctg
     6481 gcctgcgggc tctccagccc ccgcgcggag gagctgtaaa atgcgataac ctgtccggta
     6541 gagacatgac gcgcaacggc tttgaccgcc gagaggcggc cgtgctccgc aagttatcct
     6601 cacctgccaa aatccagcaa tttctcgacg ccgaaattgg atacaacaaa gagaaagacg
     6661 gcgatacgtg ccgctcgccg cggcgcgtgc tgcgcgatcg ggtcgcccat tgcatggagg
     6721 gcgctctgtt tgcagccgct gctttgcgcg tgctcggatt tccgccgctg atcgtcgatc
     6781 tcgaatccgt ccgcgacgat gaccacatcg tcgccgtctt ccaacagcat ggctgctggg
     6841 gcgccatagc caagtccaat tacgcagggc tgcgctttcg cgaacctgta taccggaccc
     6901 ttcgggaact ggcgatgtcc tatttcgagc actattacaa cccggcaggc gagaagacgc
     6961 tgcgcgccta ttcgcggccc gtcaacctga gccgcttcga ttccatcggc tggatgactg
     7021 cggaagaaga cctctgggcc attccggagt atctctacac gattccgcac cggcgcctgc
     7081 tgacggcgga aatggagcgc cgcttgaatc gcatggaccg gaggcttttt gccgccggca
     7141 gggtaggcgc tgtcgagtga gactggttgc cgctgcgggc attcgctatc gccgagatgg
     7201 agtggaaacg catccaggca ttgccgcgag tatttgccct cagaatttcg tcactgccgt
     7261 gaaatggaag ctgcgggcct tcagggcggg cacgaccatt tcgaaggcct cttcgcccgc
     7321 tgtgcgctcg gccggagaca tcaattccac gttattcagc agctcgatca tgctctgatt
     7381 gaaccggaag ttgcggaccc catgaacgat tttgccgccc tcgatgagaa acgtcccatc
     7441 gcgggtcatg ccggtgagaa ttttctcgta ggggtccact tcgcgtatat accacagcct
     7501 ggtgaccagc actccccggt cgacggagtg aatcagctga tccacgcttt ggtcgccgcc
     7561 ggaaaaaacg atgttgaccg gagcttcgcc atactcagtg ggcagcggaa atccgtgccc
     7621 ggtaggctcg acgcctgccg ccttagcggc gcggcgagag taggccacgt ttcgcacgat
     7681 gccgcgatcc accaaaacca gcggctgcct cggcacacct tcgccgtcaa acggggctcc
     7741 cgattgcagg ggatggtaga cgtcatcgca aatcgtaatg ttggcgccaa aaagctgctt
     7801 cccgatgcgg tcggtcagga agctgcgctt gtctcggaca gcggtggcgc tgaaatcgaa
     7861 agccagaaaa ccgagcaaat cgaggacggc cgctctttct aaaacgacgg tgtaagcgcc
     7921 cggctccatt ttaaccgggt ggcgtgaggc gctggcgatt tctacggcgc gcgaagccag
     7981 cgccggcgga tcgatctctt gaacccgaac cgagttggat ttcgcccatc cggaactatc
     8041 gggcgcgatg gcggtgatcg acgcctcagc gcgggtctgc gcgtggtagg cgaacagacc
     8101 ccgggaattc aggatcgcct cggccgaagc cccggtggcg aaaacgcccg ccgccgtcat
     8161 ggaatttgcc gaagccctgt caacagcctc cttcacctct ccagcgcgcc agtgggccga
     8221 cgtctcagcg gtcggttcat cgaaacgctc gaccgtttta taagtgaaag gctcggccat
     8281 gggaggcaga tccggatcgg gcgtctgaaa tttcgcaatc gccgccgctt cctcgaccac
     8341 acgccggatg gagtcatcgt ctaagcggtt ggtggttgct cgcgcggtgc gctggccgat
     8401 gacaacccgc acggagagga cctgtgtttc ctcctccatg ttttgatgga tgacgttgtt
     8461 agcgaagcgc gtgagcgcct ggcggctggc gatgagcaga atctcgaagt catcggtcga
     8521 tgagaatttt tttactcggt cgaaaatttc ctggcaacgc gagcgggaca gcagcgcaaa
     8581 ctcagacatg cgggacccca agaaaggcca tcggcaacgg cgcggcccac cgtctatttt
     8641 ggaatgggcc gcgcttgctt cggatcattg ccggccgcga gcgctttgcg gaccgtccgg
     8701 ctctatgcgt acgccgcccc gattttgaca ttgcgaaaac ggctggggct ggcgccgtgc
     8761 ccggtgccca tggtctgctg cggctggccc ttgccgcagt tgggggtgcc ccagagagtc
     8821 caatactcgg gcccgcaaat cgcgtcacat gagttccaaa attccgtcgt tataccgcta
     8881 tagctcggat tcttcagcat ccgaaccttc ttgccgtttt tgatttccca gccgatttcc
     8941 gtgccgaact ggaactggta acgcttgtcg tcgatggacc agctccggtt ggtttccatg
     9001 tagatgccgc ggtccgtcga ttcaatcagc tcttcggggg tcattttgcc gggcttcaaa
     9061 ctaacattgg tcatgcggat gagcggaata tggttccagt tgtcggcgcg catggttccg
     9121 ttcgagcgcg gctcgccgat ctccgcggcg gtctcgcggg aagtcatata gccgacgaag
     9181 cggccgtcgc ggatgatatc cgtggcttgc gcgggaacgc cttcgtcgtc aaaagcaaag
     9241 gtcccgagac caggcccgtg ctcgattcgc gcatcgcaga cgacattcac tatctcggag
     9301 gcgtattgca actttccccg cttatccaga gtcagaaagc tcatgcccgc gaagttggct
     9361 tccgccccca gaacgcggtc aagctctatc ggatggccga tcgattcatg gatctggagc
     9421 gccaactgtg aactgtcgag aatcaaatcg aacactccct cggggcattg atcggcgcgg
     9481 tgcagagcaa cggcttcttc ggcgacgcgg ggcgcgtttt cgagcagccg gagctcgtga
     9541 accagttcat agcccttcag catatgctgg ccgccgaacg aattggggta ggagcgcttc
     9601 tggatctcgt tccccgagta gctgtacgcc acgcaacccg ccccggtgta ccatcgcgtc
     9661 tgctcaatcc ggctgccttc ggaggaggca aaaaattggc gcaagcgccg gcaggtgacc
     9721 gccgcctcgg ccagcgtcac cccctgtacg cggcgcaact ccgcgtcaac tttgagcaga
     9781 agatcaagag tctgatcgac tggaacggag aagggatcaa tctgaaccgg cgaagaccat
     9841 tgcgcctcgt gcttctgctc gggagccagg gcgacgtcgc gcttctttac cagcgccgaa
     9901 gcgcgagcga tctccacggc ccgggtggcc gcggcctgga cgccctccgc tgtgagccag
     9961 tccgtggccg agaatcccca tgccccggaa gcaatcacgc ggacgcccaa gccgagactc
    10021 tccgacgaag aagcctgacc gactcggccg tttttcgtgg aaaccagccg ttccgccgat
    10081 tcgaccagtc gaacgtcagc ataggaagct cccgcctgga ccgcgcgatc gagggccagc
    10141 aaggcgaagt cttgcataga aaaaattgcg cccaaatgtg agccgctgcc gccgggcgga
    10201 aggaaggcgg gcgctcccct gccgattcca acgaaaacta agttccctcg gaccaactgc
    10261 tgaggtattt ttcctgctcc ggagtaagcc ggtcaatctt gatgcccatg gattccagct
    10321 tcatctgagc gaccttccgg tcgagctctt cgggcaccgg gtacactttt ttttcgagct
    10381 tggagtgatt cttcaccatg tactcgagag ccatcgcctg gttggcgaag ctcatgtcca
    10441 tcacggaagc aggatgtcct tcggctgccg ccaggttaat gaggcgtccc tcgccgagca
    10501 cgaacagctt cttccctccc cgcagggtga actcttcaac gaattcgcgc accgggcggc
    10561 gagaccgggc gattttgctc aggcactcca aatcgagctc gacattgaag tgtcccgcat
    10621 tgcaaaggat cgcgccgtct ttcatccgct cgaagtgctc cgccccgata atgcctttgt
    10681 ccccggtgac ggtgataaag acgtcgccga taggcgccgc gtcgagcatg gacatcacgc
    10741 gataaccatc catcaaagct tccagagccc ggatcgggtc cacttcggtg acgatgacct
    10801 gtgcgcccag gcctctcgcg cgcgaagcca ggccgcggcc gcaccagcca tatcccgcca
    10861 ccacgacatt cagcccggcg aacaggatgt tggtggcccg cagaatacca tcaatggtgg
    10921 actggccggt accgtaacgg ttatcaaata aatgtttcgt catcgcgtcg ttgacggcga
    10981 cgatgggaaa cttcagcacg ccctgcttgg ccatggcccg caaccggatc acgccggtcg
    11041 tggtttcttc cgtgccgccc agaacctcgg aagccatgtc ctggcgggcg gtgtggagca
    11101 tgttcaccag gtcggcccca tcgtccatcg tgatctgggg gcggtgctcg agggcggctg
    11161 tgatgtggga ataatacgtg gcgttgtctt cgcctttgat ggcatagacg gaaatcccgg
    11221 aatcccgcac gagagaggcg gcaacgtcat cctgcgtgga gagcgggttg gacgcgcaga
    11281 gcacgacgga cgcgccgccg tcgcgcaaag cgatcatcaa gttggcggtt tctgtcgtga
    11341 cgtgcagaca ggcggaaatc cgaattcctg ccaacggctg cgtcttaatg aactgcttgc
    11401 gaatggattg aaccacgggc atcgactgga aggcccactc gatacgcctc ttcccgagat
    11461 ccgccagtcc gatgtctttc acgtcgtggc gtacagacgt attcaccgta gacatagaaa
    11521 cgcctaatcc tcacctttca aactaaaacg acggctggct ccgggccagg cctaaacccg
    11581 ggccatcaag gccgcagcct gggcccgaaa ctcctctgcc ttgtcggtcc gctcccaggt
    11641 gaagccatcc tcgttgcggc cgaagtgtcc aaatgcggcg gtcttcttat agatagggcg
    11701 ccgcaactgg agatagtcga taatgccctt gggcgtcaat gggaaaagct cccggaccaa
    11761 ctcgctcaga tggacgtccg ctacttgccc cgttccctgc gtatccacca aaacggaaac
    11821 cggatcggcc acgccaatgg cgtaagctaa ctgcacctcg cagcgcttgg cgaggcccga
    11881 ggcgacaatg ttctttgcga tataacgggc catgtagcat gcggaacgat ccaccttggt
    11941 gggatctttg cccgaaaacg ccccgccgcc gtgacgcccc attccgccgt aggtgtccac
    12001 aatgatttta cggcccgtca gaccggtatc gccggccgga ccgccgatct caaaccggcc
    12061 cgtcgggttg atgtggatct tggtgcccga atccaccatt tcccgcggta taaccgggcg
    12121 gatgacgtgc tccaccacgt cggcctgtaa ctgagattgg tcgatatctg cgctgtgctg
    12181 ggtcgagact acaacggcat ccactctgac ggcccggtgg ccttcgtatt ccacggtcac
    12241 ctgtgacttg ccatccgggc gcaggtattt caaagtgccg aaccgccgga cgtctgagag
    12301 ccgacggacc agcttgtgcg ccaacatgat gggcagcggc atcagctctt cggtctcgtc
    12361 gcacgcaaaa ccgaacatca tgccctggtc gccagccccg tcccgatcga cgcccagcgc
    12421 tatatcgggt gactgcccgt gaatggcgtt gatcacggcg caggaatcgg agtcaaaacc
    12481 gtacttagcc cgggtgtagc ccacgccgcg gatcgtttcc cgcgccactt cggtgtaatt
    12541 tactctggcc gtcgtagtga tctcgcctgc aataaaacac agcccggtgg taacgagcgt
    12601 ctcgcaggcc acgcggccca gagggtcctg agccagcacg gcgtctaaga tagcatcgga
    12661 aacctggtcg gcgattttgt ccgggtgtcc ttctgtaacc gattcagagg taaagagccg
    12721 acgatgaaat atgggcacac gcaatctcca tccaaagcaa aacctgcggc tgccaccaac
    12781 ctgggtgccg tcttaaggcg gtaaaaattg tcgggctaaa cagaacctac cagaaaacca
    12841 gacccgccgt caatccggcg cccgtgaccg ggtaaaaacc accgggtctg agtaatttga
    12901 gctttgagtc ggaaccgatc cctaccgctt gcgaggggca aaacgcaaca tcttgttgcg
    12961 ttttgtgggg ctccaggatc tggcataggg attgcaaaac agaaaggcaa taaagccgga
    13021 aacggctgat agcatcccag gcgacgagac ccagctcgtc gccagcccct ctgagcaaac
    13081 cccggcatcc accaaggatg ccggggcact tccattttat cgaaaaaacc aaaaacgccg
    13141 caagattacg agcgttcacg gacaccccgg ggagcgccgt tcggcgtcaa gcggcatatt
    13201 tttcgtagaa aatcgatcgc gggtcggccc cgttccgctc gagtgcatcg cgaacttcct
    13261 tgaccatgct gccgggaccg caaacataaa agtcggtatg cggaccatcc ccggccaggc
    13321 ggatcacatg ctcctgcacc cgtccgcgaa ggcccctcca ctcaggtccc ccctgggtca
    13381 aggtcggata aaagcggaag ttggcgtggc gcgcagcgaa ttgctcgaac tcgctccggt
    13441 agagaatgca ttcctccgat ctggagccta agagcagttg caggtgtggg ggaacgccgt
    13501 gacgcagcag gtatccgatc atcgagcgga tcggagcaac gccggttcca gcagccacga
    13561 aaaaacaatt gcgcacaggc tgccggagct gaaagccgcc gtgcgggccc tcgaagcgca
    13621 cttcgtcccc ggatcgcatc tgaaacaggt aactggagag cggcccgccc gcaatccgtt
    13681 tcaggcacag ttcgaagaga ttgtccccat tcggagggga ggcgatcgaa tacgcgcgcc
    13741 ggatgatctg acctccggtt tcgtaatgga aacagacgaa ctgccccggc tggaaaacta
    13801 ccctttccaa ggcaggcgct tcgaatagaa agtggcgaac gtcaggggct atggggaagc
    13861 tttcaatcag gcgggcggtc tgtgcaccct cacaactcat cgttcatacg cttgccgggc
    13921 gcgctcagcg aggatttcga ctaagcccgg atgcccgtcc aacggatcgc cgcaaacaat
    13981 atcgatgtcc ttgtgagcgg cggccacctc ggctaccagg caagggaaat cccgctggag
    14041 atgaagccca aacgtcagaa aatagggtac cacgatcacg cgcctggcgc cggccgcggc
    14101 cagctgcgcc actgcggcgg ccaggttcgg ctgcccgcct tcgagaaacg ccgccgacca
    14161 caagtcgaaa ccagctttcc cggcggcagc ccggctcacc tctcgaacag cctggtttgc
    14221 cgattccacc gaagagccgt gcgcaaagaa aatgaccgcg gccttttccg gccggcgagt
    14281 gttaccgggc ggcaccgttg atatattctc tcatgtgctg gatctcttcc gccttgcgct
    14341 cgaggtcgta atacatcaga tcccgcatcg agatcatttg aatgacgcgt ccgttcctga
    14401 gcacgggcag atgacggcag ttgtggcggt gcatgacctc catcgcctcg tccgcagtag
    14461 ctgattcgtc cacggttgtc ggactgggag tcatcacttc ggccacgcgc accaggcgcg
    14521 gatcccggcc ttccaccagc acccggttca gcagatcgcg ctcgctgaag attccgcaca
    14581 attctccatc cttcagcacg agaatcgcgc cgacatgaag atcggccatt ttcgtcgcga
    14641 cttcgaagac cgtctgatga ggctccacga agtaaagctc ccgatcggac agaatttggg
    14701 agatctgcgc cataaggttc ccctcccttt tggcactgtg atcttggcgc caaaagcgct
    14761 gccagagact tagaaagagt cttcgagccc gatcctcagc cggctgcaaa cagcctgggt
    14821 tccctgttca gcgcctgcca gcaccctaaa gatatcatcg cgtgcgcccg cgagcaatat
    14881 cggcggctct atcccggaac gatcagagac ggctcaccgg aggcgcggct cccgacagcg
    14941 cgccccccgg ccagttcgat cctcccagac gaatccgctg gcccgaagtg tctgcggaga
    15001 aagtcgataa agctgcggcc ccgcttccgg ccattatccc ggtccagggc catccgcagc
    15061 agaaacacca ggatgccgag cacatcggcg tcgcgagtgc gcgtcatgcc ggtccgttgg
    15121 gtctcttcct tacgaaaggc ccgcagcgcc tcctggatgt atctggcgat ggcctgagcc
    15181 accggcgacc gcggctccgt ttcataatag actccgcttt cgagggtctt atacgtgcgg
    15241 gccagggcgt ccaaagcgtc gcgcacatcc cggtccgccg cgcctggcgt gttcagggcg
    15301 gcggccaaca cgaattctcc gagcgccgtg agcaactcct cgttttgccg cagatacgtg
    15361 tccgaaattc gtatctcttt atagggaaag ttttccggat cgagcccggg ccgcttttca
    15421 tgctcccggc tctccatcag gtaggggcag tcgagagggc aatccagcgt ctcctctcgc
    15481 tcgcggccgc agcactgggg gcaaattgcg gagcggactg cgggacaatc ccgcttggcc
    15541 ttgcggcggt gacaaatggc gcacgtcatt tcactattat cataggattt gactctcgga
    15601 agccagcgcc gttacgccat cgccgcctac ctgattctgg cttctgtcta cctgctgtat
    15661 ctgccccatg cggaattcgt gttggatgac tggctcgttt tgcgtcgata tcaggaggtt
    15721 cggcaggccg gagccgccgc ccagatcaag atgcttgtgg ccatcatcga gaaccagttt
    15781 cacggccagt tccgcttcca gtggcttagt ttttccttcg cctatctgct ctttttagcg
    15841 gtcggctact cgccgaaggt gatattcgcc gttttcctac tgttgcacgt tgcctccgcg
    15901 cttgtattgc gcgatgttct ggagcgctta aaaatcggag caggcccagc ctttctgggc
    15961 ggagctgtgt ttctgctctt gccgagcacg cacggcccgg tgttttggag ccacaactgt
    16021 tcgtattaca tctggtccac gttctggttt cttctatata tgcgctcgct ggcggcaagc
    16081 tttgcagccg gccggctcac ggcgcccgcc gccggacggc aagctttatt cctgacgctc
    16141 gcattgttct caggcgatcc gatcttctgc ctcttgctcg ccggagcgcc tttggccgcc
    16201 tggtatttgc attcgaaagc cggccgcaag gctacgttgc tcgcatggtc cacggtggga
    16261 atcatggggg cgctctatgc gctgctgatc aacagggcac gaattattca ggctggagtt
    16321 ggcctgcgct attcattcac gcacgctaat cttcggagca atgtccactc aatcctggaa
    16381 acctacggca ggctaaccgg gttcgccacc ggctctttct acgccatcca accgactttt
    16441 acggctcttg cgttcgccgc tctggcagct tcgctggtga tcttcggcct gaaaatgtgc
    16501 gaggcgcgcc gcgcgccgat ccgccgcacc ttgctgcttg ccgcaggcct gtgggccgca
    16561 gcctatggcc ccatctggtt cctgttggcc cacgagtttc gctatgatta cgtccccagc
    16621 ccctacctgg ctctcggaat cgcggcgctt gtatgctgga gccccgcgct gcgtttgccc
    16681 ctggcgggag cattgatggc ctggcttgcc gccgctaacg tggccgatat ccggcaatgc
    16741 tggataccgc aatccgacaa cctgcggctg gtcgcccgca gtttgcgcgc caccgacccc
    16801 aggccgggcg acctgatcat tgtttcccag acgcccatgt ggatcggaac cgctccaaat
    16861 ttcgcgttcc ttgcgagctg ggcgtccacg cctttcgccg agcacgtgac cggagttcac
    16921 ggtctcgaga caggaacgga catcatcgac gagggcgggc gcctgcgtgt gttccattat
    16981 gaccaccttg aggacctgac gcctgagcgc gccgcgcata cggtcctgct tgtggaagag
    17041 ggaaacgggc gtatcggccg gcgccatctg cttgcagatg aagcgcggcc aggcggctac
    17101 aggctctacg ccctgaaggg atataccggc cctgaggtcc cgtctgtcga tgtcacacgg
    17161 gagcgactct cattcctcga aggcgacatc tactttgctc gtcgctatgc ccatcataag
    17221 tatgaacact gagaggcagt ggcgagctgt tctgctcgcg gctttgctgg ttgcttttat
    17281 cggccgtatc gtcgccacct accacgtttt taatgacacg ttcgacgaaa gcgatcacat
    17341 cactgcgggg ctggagattt tggaacgcgg ccggtatacg atcgaggctc agcatccgcc
    17401 gctgagccgg ctggttctgg ccgtactgcc gtattacttc gccggcctgc gcagcgggca
    17461 ttacaacgac ctttggggtc acggcccttg ggccagccgg gacttggcgt tttattggaa
    17521 atccctctca ctggctcgca ccggcaatct ggtgttcgcg ttaatgctgt tctgtttcgt
    17581 ttatctctgg gccgcctgtc tctacggccc ctggggaggc gtcgccgcat gtgctctcgc
    17641 tgtctgctgc cccaatctca tcgcccacgc aggcctcgct accgtcgata tggccgcggc
    17701 cgccactatt ctgatgtcgg cttacttctt ttggcgctgg tgcgaacagc ccagcctcgc
    17761 ctattgcgtt ttgtcggccg tggctctcgc attggccgtt ctttcgaagc tctcgacctt
    17821 gctcttcctc ccgcccctgg gcctgcttta ttttttgctg ggccgaggga gccggctttc
    17881 ttccgtgacc ggccggggct tccggatggc gctgcgccgg gcggctgtat tcggtctgct
    17941 tgtctttatg atcgtttggg ctggatattt gttcgaggta ggcacgctgg tccctcccgg
    18001 acaccgctat caaagcccat tcggcatgca cggcggctct ggactggcag cgaccctggt
    18061 gcgctcgctc gggacgcgaa agcttcccgc ttttcgcctc ctgcaaggcg tcctcgaagt
    18121 ggctactcat aacagcagcg ggcagcgctg ctatctgctg gggcggatct actggcatgg
    18181 gtcctggctc tactttcctg tggcgattct ggtgaaaagc acgcttccta tgcttcttct
    18241 ggcggcgggt ggcgccgctc tcttcatttt cggctgcggc ctagtgagcc gccggaccat
    18301 ttatcctctc tcggcggccg ctctgatcct gatgctgagc atgccggccg ccatcaacat
    18361 cggcgttcgg catgtcctgg cgatttatcc tttcttcgcc atcttggggt cggccctctt
    18421 tactaccgga aggcgatgga tcaccctggc cgccctggcc ctgtgtgcct ggcacgccgg
    18481 agagtcgatc gccgcccacc ctgactatct cgcttacttc aacgagatcg cccgcgggcg
    18541 ggaggagcaa ttcctgatcg acagcaacct ggattgggga caggatctgg cgcggctcgg
    18601 ccagtatgtc cgggcgcgtc gcatcgattc catttacctg acctacttcg ggcggacgga
    18661 tccggccaag atggggattg ccgctatcca attgggcgat cagcgaccgg accaggggtg
    18721 cgccgccgtg agcgtcaccc gtctcatcat ggacagcaat ctggcttggc ttcgtgagcg
    18781 gaagccaatg ggccgggtcg gcaaatcgat ctggctatat gatttcagcc ggtgaaaaag
    18841 aaaatagaaa cggcctgaag cacatgtcgc ccggaaacag ccttccgagg tgcccgttcc
    18901 tattcctcta cctttggccg cacgggcttg catccagccc atgcgtgcgg gccaccagat
    18961 acaacgggcg gcgcttgatc tcctcgtaaa gccgccccac atattccccc accaagccca
    19021 acagaatgct ctgaaatccg ccgatcaacg accccacaat aacgatcgat gcccacccgg
    19081 gaacggccgt tcgaaccgag aacttgatgt aaagggcgta aatggcatag gcaaatgaga
    19141 caacccacat cagcaaaccg atatagactg ccaggcgcaa cgggaatctg gaaaaagaaa
    19201 gaatcgcgtc caaggcgagg gcggccattt tgggtcccga gtacttcgtg cggccggcca
    19261 cgcgcggcgg cgcttcgaat ggcaatgtgg cctggcggta gccggtccag ttgatcaggc
    19321 cgcgtaaaaa gcgctggcgc tcttcgagcc gccgcagttg ctcgacgacc ggccgtgaga
    19381 gcagccggaa gtcggcgctg ttgggatcga tcggcgtatc cgaaatgagg ttgatcagcc
    19441 ggtagaaaaa ggccgtcccg aggcgcttca gccggctcgc atcccgctgc acaagcctga
    19501 tggtcgaaac aatagcggcg ccgttgcgcc acaactcgac catggccggc accagctcgg
    19561 gaggatgctg caaatcccca tccatcagca ctaccgcttc gccgcaagcg aaatcgagtc
    19621 ccgcacttaa cgccgcttgg tgcccgaagt tccgcgtgaa aaccagcgct ttcacgcgcg
    19681 gatcctgctc atgcatagct gcaaggagtt cggcggtgcc gtcggttgag ccgtcgtcaa
    19741 cgaagatgat ttcctggaga tcggctccga gagcctgctt cagcctcttg acgagctctt
    19801 ggatgttctc gcgctcgttc agtaccggcg ccacgatgga aacgctaggc atgcgggaac
    19861 gggccgctga agaaatgagc aaagtaagta tcgccataca aggcgaggcg ctgtagctcc
    19921 tcaatcgtca gcggctgtgc ggggcatcgg acccccgact gcaagggcaa caactgatag
    19981 cgccggtccg gggccggcag ggcgagcagc gatttgcgga cataatggcc gccggcgtcg
    20041 cggacgtata cccgcaagcg cgagatggca tcgcgagaaa acagcacaat cgagtcgcgg
    20101 cggtaaaggg ccaagtcgcc ctgctcgttc accagcagct cacgggcgcc ctgcacctgc
    20161 ggcgcatacc ccagcagcag gctgttcaat gaccagctcg cgccggcgat gaagcttggc
    20221 gcggttccca tggcgtgcgg tgcgtgggca aaaatgaaaa tttcgcccgg tcgaatcggc
    20281 gcggcgtttt ccacagtgct tacgatggct tgcgcctcgc ggctctccgg aatccagcat
    20341 tgtcgcatct cgaaatatgt gagcgaaatc ccgtaggcga tcgccaggaa ggcgagaata
    20401 gctcgagctt gcggcggcgc ccagccggtc agggcggcag ccgccgcccc gaggaatatg
    20461 gctggaacgt agaggtatcg ccactcgatg ccgggcaacg acactacagg gaggaaggtg
    20521 agaaacgcgc cggcggcgga ccagacgagc agcctgagtg gagaaggcgc ggaagcgttc
    20581 ggctcccggc ggcggagccc aagcgcaaac gccgcgcccg ccgcaagcag aagaacaagc
    20641 agcggaatct cgggccgcca actcgggcgc cactcggcga ggctgggcct gagtcccaaa
    20701 gcggccagca agcgcagggg aatcgcccac agactcggga aattggcgtt gtggtaacgg
    20761 tgctgaaatc cccccaaaac aggcatgcga ttgagcgcca atacgtagat acttagaaga
    20821 cccataagga gcgccgtgtg aatcgcgcag taccggaaaa agcgccgatg ttttttcggg
    20881 cggccgaata tccaccagct agccggcagc aaccccacga gcaggggcaa gatctgttca
    20941 cccgtgaaga cggtcagcac caatatggca aagtccttcc acccgtaccg gtagtctccg
    21001 gctgctgcca gctttcgcca ggtgtagtaa aaccaggcgg agaaggccag ggcctgcacg
    21061 tagtaaaaac tggttgctac gggccaaaac agagcgccat ttgccgcggg aaacaccatg
    21121 tagatggccc cggcaataaa gcccgtccgg tcatcggcgg ccagcaacga cacgatgccg
    21181 gcaagaagca cagcggtgat cccatgcgcc agcagcgcca gtaagtagta gggccagccg
    21241 tgcaatcctg ctgcgagcga agcgaccagc acaggcagaa agccaaccca aaaaacgcg
//
LOCUS       DASREK010000221        23800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1587010, whole genome shotgun sequence.
ACCESSION   DASREK010000221 DASREK010000000
VERSION     DASREK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 23800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23800
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587010"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFA54_11490"
     CDS             <1..333
                     /locus_tag="VFA54_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZS51478.1"
                     /translation="LEHMRIAQWDILEFSTNPKHVSPEFPAGYWPKTEAPPGKTAWDR
                     SVEGFRSDLKAMEKLVKNAKTDLFGRIPHGDGQTILREALLVADHNSYHLGQLVVLRR
                     LLGAWPEE"
     gene            519..1334
                     /locus_tag="VFA54_11495"
     CDS             519..1334
                     /locus_tag="VFA54_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51479.1"
                     /translation="MPNRSPACFSEKRFYLPTRREALLAALGSGVLGAAELPVRKPKV
                     EILWKSPDGHPNALEATEEGLWVGDQVTDAAHLLDWRTGEVLRRVETESYNTSGIAYG
                     GGFLWLAANGALKVRPRRPADGDDAEVLKVDRATGKTLARFPLPGGAIHELNWAQNSL
                     WITARGYGKLMHVDPDFHVLHTIPLKLGRAHGVVWDRGAVWVLFSNDWVIQRLDEEDG
                     RVLEAFQLAKGADPEPHGLALYRGCLYYPDAGMAGTRPSNGKYAGYICRFAIS"
     gene            1366..1812
                     /locus_tag="VFA54_11500"
     CDS             1366..1812
                     /locus_tag="VFA54_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS51480.1"
                     /translation="MPKEIFGAPGLIAGVCMPMTPRSILGSCPAIAFVCATNPGSAKA
                     FYGDVLGLRLLRDESPFALVFDACGTMLRISFVQELKPAVHTVLGWQVPDVRAAVRDL
                     AGAGIQMRRFDGLTSQDELGVWSSPSGAKIAWFNDPDGNLLSITQF"
     gene            2002..2802
                     /locus_tag="VFA54_11505"
     CDS             2002..2802
                     /locus_tag="VFA54_11505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZS51481.1"
                     /translation="MIRPAARIVLLFTVVALGLSAQDWARQRVDKSPRHREWVTVKHD
                     GRSVETFIAYPESSNKTPVVLVIHEIFGLTDWVEDLADQLAEAGYIAVAPDLLSGMGP
                     NGGRTTSFPAGRVGEAIGHLDPNQITADLNAAADYGLKLPASNGKLSVTGFCWGGGQS
                     FRFAANRADLAAAFVFYGPPPPPEAMARIKAPVYGFYAGNDARIDATIPATRDEMKKA
                     GKTYEAVIYEGAGHGFMRAGEAPDASEGNRKARTEAWARWKELLKQKS"
     gene            3340..4128
                     /locus_tag="VFA54_11510"
     CDS             3340..4128
                     /locus_tag="VFA54_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS51482.1"
                     /translation="MSILLKTSFCFLALLAPLCAQRQRMPAPAEKNPFDTPQDVEQGA
                     GLFQTHCSYCHGSHGEGGRGADLTAGQYRYGGSDAELYATIRNGIPGSEMPPVRATDD
                     EIWRIVAFVKKIGSSGLMEKAPGDPAAGKLVYDKSGCSACHSINKQGGNLGPDLTDIG
                     RRRGLKYLEESLVKPEADVPINYRAIQVVLKSGQTVTGIRLNEDDISIQLRDTSDNLR
                     SFLKENVKEIRHDNPSLMPPYGGILSKKDLEDLVAYLNSLRGGQ"
     gene            4125..5783
                     /locus_tag="VFA54_11515"
     CDS             4125..5783
                     /locus_tag="VFA54_11515"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZS51483.1"
                     /translation="MRAHPIWTCVILIFAAAAGAAPPASGQGLPVTYERLLKADQEPG
                     NWLMYSNTYNSWRFSRLDQINTQNVNRLHVKWLFQGRHQEKFETTPLVVDGIMYLTRP
                     ENAIYALDAETGRVLWSYEYRNPPRTYNCCGKVNRGLAILGNRLFMNTLDMHIIAIDA
                     KSGRELWKTEMYDYTAAGGYAAPGAPLALKDKVIVGMAGGEHGVSGFLDAYDAATGKR
                     LWRFNTIPQPGEPNFGTWAGDSWKTGGASTWNNGSYDPETNTLYWGTSNPWPDYNGDF
                     RAGDNLYSCSVLALDPDTGKLKWYFQFTPHDLHDWDSTQIPILLDGEFRGQQRKLLAW
                     ANRNGFFYLLDRTDGKFLLAKNFVKQTWAKGFDDNGRPEVIPGNEPTPEGNDKVWPGV
                     DGGANWMSHSYSPLTKLLYVFAREERRVFTKNAVRHPTTAPEGGEGNGPAMAFFMRRP
                     PRFAPEESWGKVAAIEPMSGVIKWEHKVVSPPWGGVMATAGNLVFGGTLEGVVFALDA
                     RTGERLWYFSGNDRVYASPISFLANGRQYVSLPVGDVLITFGLD"
     gene            5848..6957
                     /locus_tag="VFA54_11520"
     CDS             5848..6957
                     /locus_tag="VFA54_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS51484.1"
                     /translation="MRLILRGVAAALAGILVLAGQSRPGRITGDAAAGKAIFEGKGGC
                     LNCHSIENHGGSLGPDLSEIGIMRTPESLRLAIIDPDAEIYQEYLTVVVTTGQGQKVE
                     GIALNEDDISIQIRDTDGTPRSFLKDNLQEVRREERSLMPSYAGKLSPSEINDLVAYL
                     RTLRGASAPAGAPVRRTRQIAPVSENIAFLTRPERDADERPESLLDALQIPSGATVAD
                     LGAGAGYFTWRLARRVGPRGKVIAVDIQQQMLDLTAAELKKRNITNVDLVLGTEQDPH
                     LAPGALDLVLIANAYHEFSQPELVMAAVRRSLKPNGRLVVLEYQKENTYAPVPSLHKM
                     SFEELRSEIESMGFQLDRVLDFLPLQHGLIFIKTP"
     gene            7411..8289
                     /locus_tag="VFA54_11525"
     CDS             7411..8289
                     /locus_tag="VFA54_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZS51485.1"
                     /translation="MTTCKLKGTNMSRIKVSALTITLLIPAIIPVQKLAAQAPARGQG
                     QAVQSPEAREAAARYAAADIRVVAPGVVYNAGLLDLAAAYTKETGKKVAVSLVGMGSI
                     VNAVKTANPPGDVIMLPFELMSTLSLDGGVVPGTFTPLGRSEMGLAVRAGAPHPDIST
                     VEKLAAALRSAKAVMRSNPAGGSMVARVIEEKVIKRPEFAGVNSPVSTRGEGGQALAR
                     GEGDMAIQAICEILPYKQIELVGPLPRELGAWIDMAVAISARAMHRDDGLAFMKYLLR
                     PESNAIWKGKALERFH"
     gene            complement(8416..9216)
                     /locus_tag="VFA54_11530"
     CDS             complement(8416..9216)
                     /locus_tag="VFA54_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51486.1"
                     /translation="MRKWACLASLILAAASVSGETSQEKGRQLVRQCLDAVGGQKFLD
                     MRDKVAHGRAYQFYESRLSGLAVVTIYTKYDRKPSSPEPGWIGVRERRDYGKNADYSF
                     LFADGKIWDITYRGARPFPETQVREYQERLRRDIFYILKYRVDEPGMIFEWGGADLVE
                     NQETEKLLVTDAGNVSLTVYLNKFTHLPMREEYLRRDPRTREVSREVSNYSRYRNISG
                     VQIACNTQVWRDDEKLAESFIEDLEINKNLDESLFTLKKGLKILPPDK"
     gene            complement(9213..9923)
                     /locus_tag="VFA54_11535"
     CDS             complement(9213..9923)
                     /locus_tag="VFA54_11535"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZS51487.1"
                     /translation="MSKPLIGLPARWEEREKGDWQKSRWYLQPTYFEAVADAGGLPVM
                     IPLIPDLAREIFARLDGVILTGSDSDVDPARYGRDRRPEVRFVLPARDQTDFALLEEA
                     DRSSRPVLGICYGMQAMNVYYGGTLVQHIPSAIHTEIDHSEHSTRHPIVVEPDSVLAG
                     LAGAGSHTVNSTHHQAVEQVAAKFHVAARAPDDVIEAIECRNGQRFLMGVQWHPERIF
                     SESAVSRNLLRRFIEACR"
     gene            complement(9920..11944)
                     /locus_tag="VFA54_11540"
     CDS             complement(9920..11944)
                     /locus_tag="VFA54_11540"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HZS51488.1"
                     /translation="MKEIEIPNPEVFTLPNGMRIYLLEDHMLPIITGYALIRTGNLFD
                     PPEKIGLAEATGAVMRTGGTVSKSGDELDQELESMAASVETGIGETSGEAHFFSLKEN
                     FDKVLAIYADVLMHPAFRQERLDLFKNQTRSAIARRNDDPAQVARREFADLVYGKDNP
                     YGWEQEYEHVDRIQRADLEHFYRRYFFPANVMLAVYGDFSTPEMKRKLEAAFKDWNNM
                     QPPAPPFPPVKASHKGGTYLAVKEDVNQTNLRMGHLAGELRDPDYPALEVMADILGGG
                     GFHSRLMEEVRTKLGYAYEVGARWGAAYDHPGLFVVAAGTKSDSTVKAAKAMIAQIER
                     MRESEVTEAELKTAKDSVLNSFVFNFDTRAKTLNRLLTYEYFGYPKDFIFQYKKKLEA
                     VTRQDVLNVAKKHLHPEELTIVAVGKPSDFDQPLSALGRPVTTLDLTIPQPKQEQVKA
                     DAQSLARGKALLEKAQKAMGGADKLAALRDVVLSVSAQLSGPMGSMAVQQNKEIVFPA
                     QLRQENTLPFGQLVVYSDGKTGWMKTPQGQMPLAGPQLEQVRGELFRQTETLLLSDRE
                     PDRTVVFVKKDVVNGREADVIEIRSKQGQQVELYLDASGQLLKKQYPGQAIAGSAPQV
                     EELLEDFREVSGIRVPFKVTVFQGGKKFSEMQVTGCRYNTGLKPEDLAKP"
     gene            complement(12112..13677)
                     /locus_tag="VFA54_11545"
     CDS             complement(12112..13677)
                     /locus_tag="VFA54_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZS51489.1"
                     /translation="MFASRASGLRYLFLAGLLVLPARPQNLKEFEKSVTEFTLPNGLH
                     FIAVERHEAPVVSFYTYANVGSVDDPRGATGLAHMFEHMAFKGTERIGSKNWPAEKKA
                     LNAVDQAFSDLMAEKNKGRLADRAKVEQLEKAFQAAVEKAGELAESNDYSRIIEENGG
                     VGMNAATGPDSTVYFYSLPSNRAELWFYMESERFLHPVFREFYKERDVVREERRMRTD
                     SNPVGKLVETFLGQAFIAHPYRQPPVGWPSDLEYLNVSEAKQFFDTYYVPNNLTIAIV
                     GDIDPQQIRRLAVSYFGRLERRPLPPAIHTVEPPQEGTRRVEVESPAQPVVILGYKKP
                     SEYDPDRAVFDVISSVLSGGRTGWLYKELVRDQQISLDAGGFPHFPGDKYPGLFVFYS
                     FPTSGHTVEENEKAMYGLIEKIKTERVDTDTLNMVKTKNRAELIRKLESNADLAGELA
                     SYYANFGDWRQLFYSLDEINRVTAEDVQRVAKKYFRPESLTVAYLVAPPQNPARHDGE
                     QSGPQHQQGAPQQ"
     gene            13894..14769
                     /locus_tag="VFA54_11550"
     CDS             13894..14769
                     /locus_tag="VFA54_11550"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZS51490.1"
                     /translation="MRRLAAFLAPTGKFLLQTEIHAYAFAISANLLLSFFPFLVLMLS
                     ICQNLLHWPRAADVIYLALQDFLPSDPGVLDFVRRNLRAAVSAHGRIAVFSAILLLAT
                     SNGVFEPLEVALNRVWGIGSNRSFWRNQWVSFGLILVCGALAFISILFTTMNRELLDA
                     LAGPLPALARWASLAAFKAAALPVSVAILFLVYWLLPNGLIPWRSALTAAIVIGVVME
                     AAKYLYILVWPLLDFRRVYGPFFISVTVIVWGVAASLILLAGAEISARLQRERAGRAA
                     AGQAFPQKQIPKNLF"
     gene            complement(15105..15590)
                     /locus_tag="VFA54_11555"
     CDS             complement(15105..15590)
                     /locus_tag="VFA54_11555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HZS51491.1"
                     /translation="MKLDSPNLSAGSGADSQRSFPETCLFCRIILGKAEAYRVFEDSV
                     CLAFLDHRPLFPGHSLLLPKQHYETLADLPGNLLGPLFSGAQLLSRAMELGLEAEGSF
                     VAINNRVSQSVPHLHIHIVPRRRKDGLKGFFWPRQAYKDREERLHVQQAIQAAVARLR
                     A"
     gene            complement(15621..15956)
                     /locus_tag="VFA54_11560"
     CDS             complement(15621..15956)
                     /locus_tag="VFA54_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51492.1"
                     /translation="MRNLGFVLAVFVCLWPPAGHADQQTGSFAGPHGVFSVTGGTFKP
                     SESFEPASGSFSVPAGTFQPSKEFHPAPGSFSVPPGDFKPAAGEFKPTASFRVEPGTF
                     SSSGFLSKK"
     gene            complement(16219..17172)
                     /locus_tag="VFA54_11565"
     CDS             complement(16219..17172)
                     /locus_tag="VFA54_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51493.1"
                     /translation="MQPSRNPGLPPLIFAENPSRRKKFIRGAAWASIAALLGVGAYRY
                     SFPEIRKQADLLARLPGLQQSAAAAQQRIRAAEDRLAALSADQDGVAARVTRLESRVT
                     ANLQTARKQTQEIVAQAQQHIEAEHAGRMDALQARLGVVEASQGEEQTRLVQLQNEVS
                     AAREQIARQSQQIAFLQQETGRQLDGVSRQVARLDRDADRERRNLGSLSAKVERKRID
                     FEIAKNHSREVAPGVTIEVTRIDVGYRKFDGWIWLLPDRRTVWVRQQSAQRPVIFYTK
                     DDNRPRELVITHVTKNSVAGYLLAPAPGYDGSSPDAIAALQ"
     gene            complement(17395..18525)
                     /gene="mqnE"
                     /locus_tag="VFA54_11570"
     CDS             complement(17395..18525)
                     /gene="mqnE"
                     /locus_tag="VFA54_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HZS51494.1"
                     /translation="MPLLEPSVIDDPSLKPIAEKVLASERLTYKDGVTLYRSHDILTI
                     GYLANLVRERKSGNVTYFNVNRHINPTNVCVAACKLCAFGRQVRDPKAYTMSLEEVWH
                     TAGSGYTEAVSEFHIVGGLHPELTLDWYCEMIAGLKQRFPQVHLKAFTMVEAGYLARR
                     SRLSIEETLRRLKQAGVDSMPGGGAEIFVERVRRIICDHKISGEEWLETARTAHRIGL
                     HSNATMLYGHIETDEDRVDHLLKLRALQDETRGFVTFIPLAFHPANTPLQHISPTTGF
                     ADIKNVAIARLLLDNFPHIKAYWIMMTPKIAQVAQRFGADDIDGTVVEEKIYHDAGAT
                     TAQSLRRSELLHLIRRAGRDPVERDTLYRPVSRNEATFTVLV"
     gene            complement(18512..19393)
                     /locus_tag="VFA54_11575"
     CDS             complement(18512..19393)
                     /locus_tag="VFA54_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HZS51495.1"
                     /translation="MRALRKLRITLEMIKFEHSVFALPFALTGALLAAREEKLSLAAV
                     GGKLGWVVLAMVSARSAAMAFNRLADAQIDARNPRTRMRAIPSGVLSRSFTAGFVAVA
                     SATFLLAAGKLNALCLKLAPLALGIVFFYSYTKRFTAFSHLILGFCLGIAPAAAWIAV
                     RGRLDAPILLLTAAVMLWTAGFDIIYSCQDYDFDRRERLHSMPARLGIAPALWVARLL
                     HAAMVVLLVWLAEALSLGPLSLAGVGLVTALLIYEHSLVRPGDLSRVNAAFFTVNGVV
                     SVLFFLFWAADILYAAA"
     gene            19446..20216
                     /locus_tag="VFA54_11580"
     CDS             19446..20216
                     /locus_tag="VFA54_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZS51496.1"
                     /translation="MKIQSLVIGAAAMLAAAALGLLSGGRAFSSPQAGSGQRQPASGS
                     LIEGFRLVEVASVADAMEQLYGERTYMSHQMRPLFPTKFAGPAVTVLMKKEEHKEGAP
                     ASQGMLDAIDAAPPGSVYVMVMPEGADFAGMGGLMATAMKFRGLAGAIIDAGIRDTPQ
                     IRKLQFPVFSRGVVPSTSINHYRFAGANVPVNCAGVQVNPSDIIVADEDGVAVVPRTR
                     AAAVLRRAQELDDTEHRMLPFIEKFRSIKEAVAKFGRI"
     gene            complement(20283..21008)
                     /locus_tag="VFA54_11585"
     CDS             complement(20283..21008)
                     /locus_tag="VFA54_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZS51497.1"
                     /translation="MHVTRAVILAAGRGSRMQTLTAGVPKPMLEVRNKPILAHLIINL
                     KTAGLRHILIVTGYCAEQIEAYFVREPGVSFCRQEVPDGTARAAELARDFAREEPFLL
                     TYGDILVAPEAYRETANRMDGFEAVLAVKYVDDPYQGAAVYIEGDRVNDIIEKPPRGT
                     SATHWNSAGFYCFRPSIFEQLAGVSVSPRGEYELTDGIRTLLAKGLPVGYYPIPGWWR
                     DVGRPEDLAAAEQMLAFPDASPR"
     gene            21173..21580
                     /locus_tag="VFA54_11590"
     CDS             21173..21580
                     /locus_tag="VFA54_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51498.1"
                     /translation="MRFCRRIIVVALCCAAAVAMAATSPSGEVVLHGKIRQEAGKPPV
                     IETADHQVYTVSGDEFLTAQMADSRLNGRDIVLEGHVTGPGKFEASHLYTIKNGKRYE
                     VTYWCDVCSIRTHMPGRCMCCQGPTELQELPVP"
     gene            complement(21751..22203)
                     /locus_tag="VFA54_11595"
     CDS             complement(21751..22203)
                     /locus_tag="VFA54_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51499.1"
                     /translation="MSRKQYIFVLLLAVAGGVAGGAFVSRLLVARPAFAQATEENSKV
                     LRAQRFEVIDEEGKRRGRFDSLPNGAVRLVFYDNDGKIRTGLLVATNGTPGLQFWDKD
                     GRPVSVFSDGVTLLDPAGQPRGRMSVSASGNPSITFYNADGKVTWSAP"
     gene            complement(22434..>23800)
                     /locus_tag="VFA54_11600"
     CDS             complement(22434..>23800)
                     /locus_tag="VFA54_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZS51500.1"
                     /translation="ATEDQLEFPVLYCNARTGRAGLSAAEPGSDLQPLFETILHSIPP
                     PSAEPQGRLQLLVANLDYSDYLGRIAIGRVFQGELRAGEEVGIAKRDGSVQKTRITKL
                     FSFEGLKRTEIETARTGDIVAIAGIEGITIGETITSAETPAPLEPLHIDEPTIGMVFT
                     VNTSPFSGREGRFVTSRHLRERLDKELLTNVSIRVEPMSSTDCFQVMGRGELQLAILI
                     ETMRREGYELAVGKPQILTRLIGGVLSEPVETLVVDCAEEFAGLVIEMLGIRKGQMTR
                     MVNNGSGRVRMEFTIPSRGLIGLRSQLMTDTRGTAVMHSLFEGYTPWQGEIPMRPTGA
                     LVADRPGRTTGYALYNLQERGELFVGPGVEVYEGMIIGENSRAADLDVNVVKEKKLTN
                     MRASVADEAIRLIPFRTLNLEQAIEFVNEDELVEVTPQSIRLRKKILETNRRPKRKVA
                     DSGS"
BASE COUNT         4580 a         7106 c         7217 g         4897 t
ORIGIN      
        1 ctcgagcaca tgcgcatcgc gcagtgggac atactggaat tcagcaccaa tccgaaacac
       61 gtttcgccgg aattcccggc aggctattgg cccaagaccg aagcccctcc cggcaaaacc
      121 gcctgggata ggagcgtgga aggttttcgc tcggacctga aagccatgga gaagctggta
      181 aagaacgcga agaccgacct tttcggccgc atcccgcacg gcgacgggca gactattctg
      241 cgcgaggcgc ttctggtcgc tgatcataat tcatatcatc tcgggcaact tgtggtgctc
      301 cggcgccttt tgggcgcttg gccggaggag tgatggcacg cgctttggtt ggctgaagcc
      361 acagaatggc gcaggaggag cagccgccct ttgcaaaagc gacggccgcg ccctcacgaa
      421 gcaacgaaat ccgggccaat cggcttgaca gccactgtcc cccggtgata tcaccagact
      481 gccgccgctg gtcgacgtgc ggagaaaagg agcgcgcgat gcccaatcgg tctccggctt
      541 gcttctcgga aaagcgtttc tatttgccca ccagaaggga agcgctgctg gcggcgctag
      601 gatcgggcgt gctgggggcg gcggaacttc ccgttcgcaa gccgaaagtc gagatcttgt
      661 ggaagtcccc cgacggccat cccaatgcct tggaagccac cgaggaaggc ctctgggtcg
      721 gcgatcaagt gactgacgcc gcccatctgc tggattggag aacgggagag gtgctccggc
      781 gcgtggagac ggaatcttac aacaccagcg gcatcgccta tgggggcggg ttcctatggc
      841 tggcggcgaa cggagcgctc aaggtacgcc cgcgccgccc cgcagacggc gacgatgctg
      901 aggttctcaa agtagatcgc gcgacgggga aaacccttgc caggtttccc ttgccgggcg
      961 gcgcgattca tgagctgaac tgggcgcaga atagcttgtg gatcacggcc cgcggctacg
     1021 gaaaattgat gcatgtggat ccggatttcc acgtcctgca cacgatcccg ttaaagctgg
     1081 gccgggcgca cggcgttgtg tgggaccgtg gggccgtgtg ggtgctgttc tccaacgact
     1141 gggttatcca acggctggac gaggaggatg gccgcgtctt ggaagcgttt caactcgcaa
     1201 agggcgctga tccggagccg cacggcctcg cgctctatcg gggctgcctg tattatccgg
     1261 atgcaggcat ggccggcacg cggcccagca acggcaagta cgccggttac atctgccgct
     1321 tcgccatttc ctagagctca tggcggacct gcccattgcg gcgccatgcc gaaagagatt
     1381 ttcggtgcgc ctggcctgat tgcgggagtt tgcatgccga tgacgccgcg ttccattctt
     1441 ggctcctgtc cggcgatcgc cttcgtttgc gccaccaatc ctggcagcgc caaagctttt
     1501 tatggggatg tcctcggact tcggctcttg cgcgacgaat ctccatttgc gttggttttt
     1561 gacgcctgcg ggacgatgct gcgcattagc ttcgttcagg agttgaagcc cgccgtacat
     1621 acggtattgg gctggcaggt ccccgacgtc agggcggccg tgcgggacct ggcaggcgct
     1681 ggaatccaga tgagacgctt tgacggattg acctcccaag atgaactggg tgtctggtca
     1741 tcgcccagcg gggctaaaat cgcgtggttc aatgacccgg acggcaatct cttgagcatt
     1801 acgcagtttt gaccgccaga aacctgtttg cggccggccc accggctgac gccgggcgga
     1861 tgatccggga gccgcccaac gagccggggc ttcatcgctt acaatgacag ggaaaacgat
     1921 gcgccggcat gattggcagc tctttccgtc ggctccgccg gtccgcaaat cgtgccgccg
     1981 catcacacgg agggaaaaac catgattcgc cccgcagcaa ggatcgtttt gcttttcacc
     2041 gtcgtggcgc tggggctgtc cgcgcaggac tgggcgcgcc agcgagtgga caagtctccc
     2101 cgccatcgcg aatgggtgac tgtgaaacat gatgggcgct cggtggaaac gttcatcgca
     2161 tatccggagt cgagcaacaa aactcccgtt gtgctggtga tccacgaaat ctttggcctg
     2221 acggattggg tggaggatct cgccgatcag ctcgcagagg cgggttacat tgctgtagct
     2281 cctgaccttc tgtcgggcat ggggcccaac ggcggccgca cgacatcctt tcctgcgggc
     2341 cgggtagggg aggccatcgg ccacctcgac ccgaatcaga tcaccgctga tctgaacgcc
     2401 gcggcggatt atggcctcaa gctccccgcg tctaacggca agctttctgt cacaggattt
     2461 tgctggggcg gcggtcagag cttccggttt gccgcaaatc gcgccgatct tgccgcagct
     2521 ttcgtttttt atgggcctcc gccgccgccg gaagccatgg cgcgcataaa ggcccctgtt
     2581 tacggctttt acgccggcaa tgacgcgcgc atcgacgcca caattccggc cacgcgggat
     2641 gaaatgaaga aggcgggcaa aacctacgag gctgttattt atgaaggagc ggggcacggt
     2701 ttcatgcgcg cgggggaagc cccggatgca agtgagggca accggaaagc ccgcacagaa
     2761 gcttgggcgc gctggaagga attgctgaag caaaaatcct aacctctggc ggcggccccg
     2821 gcgtaattag ggatcacaac tccccgcagg ggccgatgaa tcgctccagc ctaggcggct
     2881 tcgcacgcaa ggcgcccgcg gataattgac ctggatctcg tgaagccaaa ggccgcgcct
     2941 gggcccgcca ggaagtgctt cgggccggcg ccgctgaaaa agcgcccgtt ttcccggatg
     3001 gcggcctgga cgtgctaacc ggccaatcgg ctcgaacgcc ttatccgaac gaggttcctg
     3061 gagcggactt ctcctgttct cgggggcgac gcttcgctga aaggatccgg tggccgctct
     3121 gcgggcgaag aaccgcgctc tggggcagcc gtgctgccgg ctcctctcgc tccggtggat
     3181 ttttgccatc catttcatga ggagttcctg cggggggcca cagctaattt gctggcggag
     3241 cttccgctaa cccttgcttg ggccggattt ttcaaaagca agcaggcgtt gtatactttc
     3301 agctacccgg gagcgggaaa ggaaaatcct ggatgcaata tgtcgattct cctgaaaact
     3361 agcttctgtt ttctggcgct gctcgcgccg ctttgcgcgc agagacaacg gatgcctgcg
     3421 cccgcagaga aaaatccttt tgacacgccg caggatgtag aacagggtgc agggttgttt
     3481 caaactcact gctcttactg ccatgggtcc cacggagaag gcgggagggg ggctgacctc
     3541 acggccggcc agtatcgcta tggcggctcg gacgcggagc tttacgcgac cattcgcaac
     3601 gggattcccg gttcggagat gccgccagtc cgcgcgaccg acgatgaaat atggcggatt
     3661 gtcgcgttcg taaagaaaat cggatcgtcg gggcttatgg agaaggcccc gggagacccg
     3721 gcggcgggaa aattggttta cgacaaatcg gggtgctccg catgccattc catcaataaa
     3781 caaggcggca atctggggcc ggatctgacg gacatcggcc gccgccgggg gctcaagtac
     3841 ctggaagaat ctctggtcaa gcccgaagcg gacgtgccga taaactaccg ggctattcag
     3901 gtggtcctga aatctggcca gaccgtaacc gggatccgcc tgaacgaaga cgacatttcc
     3961 attcaattgc gggacacgag cgacaatctt cgttcgttct tgaaagagaa tgtaaaagag
     4021 atccggcacg ataacccgtc gctgatgcct ccgtacgggg ggatcctcag caagaaggac
     4081 ctggaagatt tggtggctta cctaaattct ttgcgggggg ggcaatgaga gcacatccga
     4141 tctggacctg cgtgattctg attttcgcgg cggccgccgg cgcagcgccg ccagcgagcg
     4201 ggcaaggttt acctgtcact tacgagcggc ttctgaaggc cgatcaggag ccgggcaact
     4261 ggctgatgta ttcgaacacc tacaacagtt ggcggttcag ccgtctcgat cagatcaaca
     4321 ctcagaacgt caaccggctg cacgtcaagt ggctctttca ggggcggcac caggagaaat
     4381 tcgaaacgac tcccctcgta gtggatggca tcatgtacct gacccggccg gagaatgcga
     4441 tctatgcgct ggatgcggaa acaggacgtg tgttgtggag ttatgaatat cgcaatccgc
     4501 cgcgcactta caactgttgc ggcaaagtga atcggggctt ggccattctc ggcaaccgcc
     4561 tgttcatgaa cacgctggac atgcacatca tcgccatcga tgcaaagtca gggcgcgagt
     4621 tgtggaaaac cgagatgtat gactacacgg cggcgggcgg ctacgcagcg ccgggagcgc
     4681 cccttgccct caaggacaag gtgattgtcg gcatggccgg cggcgagcac ggggtctcgg
     4741 gattcctgga cgcctatgat gccgctaccg gcaaacgcct ctggagattc aacaccatcc
     4801 ctcagccggg cgaaccgaat ttcggaacct gggccggcga ttcctggaaa accggcggcg
     4861 cttccacctg gaacaatggc tcgtacgatc cggaaaccaa cacgctgtat tggggcacca
     4921 gcaatccgtg gcccgactac aacggagatt ttcgcgccgg cgacaacctc tactcgtgct
     4981 ccgtgctggc gctggatccg gatacgggaa aactaaaatg gtatttccag tttacgcctc
     5041 atgatctgca tgactgggat tccactcaaa tcccgatcct gctcgacggc gagtttcgag
     5101 gccagcagag aaagctgctg gcgtgggcta accggaacgg ctttttttat ctgctggacc
     5161 gcaccgacgg caagttcctg ctggccaaaa atttcgtaaa gcagacctgg gccaagggtt
     5221 ttgatgacaa tggccgtcca gaggtgatcc ccggcaatga acctacgccg gaaggaaatg
     5281 acaaggtatg gcccggggtg gacggcggtg cgaactggat gtcgcatagt tacagcccgc
     5341 tgacgaagct gctctatgtc ttcgcccgcg aggagcgccg tgtgttcacg aaaaacgctg
     5401 tccggcatcc aactacggcg ccggaaggcg gcgagggcaa cggcccggct atggctttct
     5461 tcatgcggcg gccgccgcgg ttcgctccgg aagagagttg ggggaaggtg gcggcgatcg
     5521 agcccatgag cggcgtcatt aaatgggagc acaaagtagt ctcgcctccc tggggcggtg
     5581 tcatggccac tgccggcaac ctggtgttcg gcggaactct ggaaggagtc gtctttgccc
     5641 tcgatgcccg cacgggcgag cgcctgtggt atttctccgg caatgatcgg gtttatgctt
     5701 ctcccatcag ctttctggcg aacggaaggc agtacgtttc gctgcctgtc ggcgatgtac
     5761 tgatcacctt tggcctggat tgataatgga tcccgagggg cggcgggcgt gagtcgtggt
     5821 tccggctccg cacgccttat cccatctgtg cgattgattc tcagaggcgt tgcggcggcg
     5881 ctcgcgggta ttctagtcct ggccggccaa agcagaccgg gccgcatcac cggcgatgcg
     5941 gccgccggca aggccatctt tgagggcaaa ggcggctgcc tcaactgcca ctccatcgaa
     6001 aatcacggag gaagccttgg ccccgatctc agcgagatcg gcatcatgcg cacgccggag
     6061 tctctccgtc tcgccatcat cgatcccgac gcagaaatat atcaggaata cctgacggtc
     6121 gtagttacaa ccgggcaggg gcagaaggtc gaaggcatcg cgctgaacga ggatgatatc
     6181 tcgattcaaa tccgggatac cgatggaacc ccccggtcat ttctaaaaga caatcttcag
     6241 gaggtgcggc gcgaggagcg ctctctgatg ccctcctacg ctggaaaact ctccccctcc
     6301 gaaatcaacg atctggtcgc gtatctccgg acgctgcggg gcgcttcggc gccggccggc
     6361 gctcccgtca gaagaacccg ccagatagcg cccgtttcgg aaaacattgc gttcttgacc
     6421 agaccggagc gcgacgctga cgagcgacct gagtcgctcc tggatgcttt acaaattccg
     6481 agcggcgcaa cggtggcgga tttgggcgcc ggcgccggct acttcacttg gcgccttgcc
     6541 cggcgcgtcg gcccccgcgg gaaagtgatt gccgtggata ttcagcaaca gatgctcgat
     6601 ctcaccgccg cggagctgaa aaagcgaaat atcaccaatg tggatctggt gctgggcacg
     6661 gagcaagacc cgcacctcgc gcccggcgcc ttggatctgg tgctcatcgc caacgcttac
     6721 cacgagtttt cccagccgga gctcgttatg gccgccgtcc gccgctcgtt aaaaccgaac
     6781 ggccggctgg tcgttttgga ataccaaaag gaaaatacgt atgctcctgt gccgagtttg
     6841 cacaaaatga gctttgaaga gctgcgctcg gaaatcgagt ccatggggtt tcaactcgat
     6901 cgggtgctcg attttttgcc cttacagcac ggcctgatat tcatcaagac tccatagcac
     6961 tctccacgcg gttgcgggaa ttgtgactgg tgttgagtat aggatctccg ccgctctctg
     7021 cctgctttcc ggaagtttga agggcagagc gccggcttcc taaggtaggt tcccagggat
     7081 gcgggtatcc tgcgccgcgc ggcagcccgg catgtcgagg tctacacggc ggttgtgttg
     7141 cgtggtctgg gactgtctgc cgaactattc gcgccgacct tcgccgccgg acgcgtcgct
     7201 ggctggtccg cgcattgctt ggagccgctc cgagaagggc gcctgatctg gctgcaatcc
     7261 atctgtacgg ccgctaaatt tccactacaa tcgcccggtt gacggcgcaa agtcttggcg
     7321 gctcggctca agtcctacgg ggcagtacaa caacgcctgc cggttttccc gccggcggcc
     7381 cccgaatgat gtataaatga cgatgaacag atgacgacat gtaagctgaa aggaacgaac
     7441 atgagccgca tcaaggtgtc cgctctcact attacgctgc tgataccggc gatcatcccc
     7501 gtacagaaac ttgccgctca agcgcccgcg cgcggccagg gacaagctgt ccagtcgccg
     7561 gaagcgcggg aggcggccgc ccggtacgcc gccgctgaca ttcgggtagt ggctccgggc
     7621 gtggtttata acgccggctt gctggacctc gccgcggctt acaccaaaga gacaggtaag
     7681 aaggtcgccg tgagcctcgt cggcatgggc agcatcgtca atgccgtcaa gaccgcgaat
     7741 ccgccggggg acgtgatcat gctgccgttc gaactgatga gcacgctgtc gttggacggc
     7801 ggcgtcgtgc cgggcacatt cactccgctg ggccgctcag aaatgggcct cgctgtgcgc
     7861 gcgggcgccc ctcatcccga catctcgacc gtcgaaaaat tggctgccgc gctgcgcagc
     7921 gcgaaggccg taatgcgttc gaatccggcc ggcggcagca tggtggcgag agtaatcgag
     7981 gaaaaggtga taaagcggcc ggaattcgcc ggcgtcaaca gcccggttag cacccggggg
     8041 gaaggcgggc aggcgctggc gcgcggagaa ggcgacatgg cgattcaggc gatctgtgag
     8101 atccttcctt acaagcaaat cgagctcgtg gggccgctcc cgcgcgagct tggagcctgg
     8161 atcgatatgg ccgtagcgat ttcggcccgg gcgatgcacc gcgacgacgg tctggcgttt
     8221 atgaaatatc tgctccgtcc ggagtccaac gccatctgga aggggaaagc gttggaacgt
     8281 ttccactgac gccgcgcccg gcagcgtccg tctccagccg catcggagac agaggatctg
     8341 ccgcctcgta tcgccgcagg cgaaaggcaa gcagcagatt ccccggtgcg ctcgattggt
     8401 ttcgagggcg gaggcttatt tatccggagg cagaattttt aagcccttct tcaaggtgaa
     8461 aagcgactcg tcgaggttct tattgatctc caggtcctcg ataaaggact ccgccagttt
     8521 ttcgtcatcc cgccagacct gcgtgttgca agcgatctgg acgccggaaa tattccggta
     8581 ccgggaatag ttgctgactt cccgcgacac ctcgcgcgtc ctgggatcgc ggcggagata
     8641 ctcttcccgc atgggaagat gagtaaattt gttcagatag acggttaacg aaacattgcc
     8701 ggcatcggtg acaagaagct tctctgtttc ctgattctct accaggtctg cgccgcccca
     8761 ttcaaagatc atgcccggct cgtccacccg gtatttgagg atgtagaaaa tatcccgccg
     8821 cagccgctcc tggtattcgc gaacctgcgt ttccggaaag gggcgggcgc cgcgataggt
     8881 gatgtcccat attttgccgt ccgcgaatag gaagctatag tccgcgttct tgccgtaatc
     8941 gcgccgctcg cgcacgccga tccatcctgg ctcgggcgaa gacggcttgc ggtcgtactt
     9001 ggtatagatt gtgacgacag ccagcccgct tagccgggat tcatagaact gataggcgcg
     9061 cccatgcgcg accttgtcgc gcatatcgag aaacttctgg cctcccaccg cgtccaaaca
     9121 ttgccggacc agttgccggc ctttttcctg cgaggtctcg cctgaaacag aggccgccgc
     9181 caatatcagg cttgctaaac acgcccattt cctcatcggc atgcctcgat aaaacgccgg
     9241 agcaaattcc ggctcacggc gctctcagag aaaatacgct ccgggtgcca ttgcaccccc
     9301 atcaagaacc gctggccgtt cctgcattcg atcgcctcaa tcacatcatc tggcgcacgc
     9361 gcggcgacgt gaaacttggc agctacctgt tcaacggcct gatggtgcgt gctgttgact
     9421 gtatgcgatc ccgcgcctgc caaaccggcc agcacggaat ccggttccac gacgatcggg
     9481 tgccgtgtgc tgtgctcgct gtgatcgatt tccgtatgaa tggcggaggg gatatgctgg
     9541 accaaggtgc cgccgtaata cacattcatc gcctgcatgc cgtaacagat cccgagcaca
     9601 ggtcgactcg agcggtcggc ctcttcgagc aatgcgaaat ccgtttgatc ccgcgccgga
     9661 agcacgaatc tcacctccgg ccgccgatct cggccgtacc gggctgggtc tacgtcgcta
     9721 tcggagccgg taagaatcac gccatccagc cgcgcgaaga tttcacgcgc cagatcggga
     9781 atcaggggga tcatcactgg cagtcctcca gcatcggcca cagcctcaaa atacgtgggc
     9841 tggagatacc agcggctctt ttgccaatcc cctttttccc tctcttccca acgggcggga
     9901 aggccgatca gcggcttgct cacggctttg ccaaatcttc cggcttcaaa ccggtattat
     9961 atcggcagcc ggtcacttgc atttccgaga acttcttgcc gccttgaaac accgtgacct
    10021 taaacggaac acgaatgccg ctcacctcgc ggaagtcttc gagcagctct tccacctgcg
    10081 gcgctgagcc ggcgatcgcc tgacctggat attgcttctt caacagttgg ccggaggcgt
    10141 ccaggtaaag ctcgacttgc tgcccttgct tggatcggat ctcaatcaca tcggcctcgc
    10201 gtccgtttac cacgtctttt ttaacaaaaa ccacggtccg gtcgggctcg cggtcgctga
    10261 gaagcaatgt ttcggtttgg cgaaacaact cccctcgcac ttgctccagt tgggggccgg
    10321 cgagaggcat ttgtccttgc ggagttttca tccaaccggt ctttccatcc gagtagacca
    10381 caagctgccc gaacggcagc gtgttttcct ggcgaagctg ggcagggaaa acgatctcct
    10441 tgttttgctg gacggccatg ctgcccatag ggccgctcaa ttgagcgctc accgagagca
    10501 cgacgtcccg cagcgcggca agcttatcag ctccgcccat ggctttttgc gccttttcga
    10561 gcagcgcctt gccccgcgcc agagattgtg cgtcggcttt gacctgctcc tgcttcggct
    10621 ggggaatcgt gaggtccaat gtggtcacgg gccgtcccag ggcggagagc ggctggtcga
    10681 agtctgacgg ctttccgacg gctacgatgg tcaactcctc agggtgcaga tgcttcttcg
    10741 cgacattcaa gacatcctgc ctggtgacgg cttccagctt tttcttgtat tggaaaatga
    10801 agtctttggg atagccgaag tattcgtagg tcagcaggcg gttcaacgtt ttggcgcggg
    10861 tatcgaagtt gaaaacgaag ctgttgagta cgctgtcctt cgcggtctta agctccgctt
    10921 ccgtgacttc gctctcgcgc atccgttcga tctgcgcgat catcgccttc gccgccttga
    10981 ccgtggaatc cgattttgtg ccggccgcca ccacgaacag gccgggatgg tcgtaagcgg
    11041 cgccccaccg ggcgcccact tcataggcgt agcccagctt tgtccggacc tcttccatca
    11101 ggcggctgtg gaatcccccg ccgcccagaa tgtccgccat gacttccagc gctgggtaat
    11161 ccgggtctcg cagttcgccc gcgaggtgcc ccatgcggag attcgtctgg ttgacgtcct
    11221 cttttaccgc caggtatgtg ccccctttat ggctcgcttt caccggcggg aacggggggg
    11281 ccggcggctg catattgttc cagtccttga acgccgcctc cagcttacgc ttcatctcgg
    11341 gtgtggaaaa gtcgccatag acggcgagca tcacgttggc ggggaaaaag taccggcggt
    11401 agaagtgctc cagatcggcg cgctggatcc gatccacgtg ctcatattcc tgttcccatc
    11461 cgtagggatt gtcctttccg taaaccaaat cggcgaactc ccggcgggcg acctgggccg
    11521 gatcgtcatt acgccgggcg atcgcgctgc gcgtctgatt cttgaacagg tcgagccttt
    11581 cctgccggaa ggcaggatgc atgagaacgt cggcgtagat ggccagcact ttgtcgaaat
    11641 tttctttcag cgagaaaaaa tgagcctcgc cgctggtttc gccgatgccg gtttcgaccg
    11701 acgccgccat actttccagt tcctgatcca gttcgtcgcc ggatttggaa accgtcccgc
    11761 ccgtgcgcat taccgcgcca gttgcttccg ccaggccgat tttctccggc gggtcgaaca
    11821 ggttgcccgt gcggatcaag gcgtaaccgg tgatgatggg cagcatgtgg tcttcgagca
    11881 aatagatccg catgccgttg ggaagggtga agacttccgg attggggatt tcgatttcct
    11941 tcaacggggg gaatctgctc tcaatctcct tcacgctgga ggcgggcggc ggcgcggagc
    12001 gagcggtttt tgcggaagct ttcggcgcgg gcgcccttct gacggcggat tgcggggagg
    12061 cgcatagggt tcccaaggcc gcccagacga ggaaggcaat cgggattcgc atcattgctg
    12121 gggcgctcct tgctggtgtt ggggcccgct ttgttcacca tcgtgccgag cagggttttg
    12181 cggcggggcg accaggtagg ctacggtcag actttccggc cgaaagtact ttttagccac
    12241 gcgctgtacg tcctcggccg tgactctgtt gatttcgtcg agggaataaa agagctgccg
    12301 ccagtcgccg aaattggcgt aatacgacgc cagttctccg gccagatcgg cgttgctttc
    12361 gagctttcgg atcagctcgg cccgattttt ggtcttgacc atgttcagcg tgtccgtatc
    12421 gaccctctcg gtttttatct tttcaatcag gccgtacata gctttctcat tttcctcgac
    12481 cgtgtgtccg ctggtcggaa aagagtagaa cacaaacagc ccgggatatt tgtctccggg
    12541 aaagtgggga aagccgccgg cgtcgagcga aatctgctga tcgcggacca gttccttgta
    12601 gagccagccg gtgcgtccac cgctgagcac actcgaaatc acgtcgaaaa cggcgcggtc
    12661 cggatcgtac tcggaaggtt tcttatagcc gagaatcacc accggctgcg cgggcgattc
    12721 gacctctacc cggcgcgtgc cttcttgcgg cggctcgaca gtgtgtatcg ccggaggcag
    12781 aggccgccgt tccaggcggc cgaaatagga aacagccagc ctgcgaatct gttggggatc
    12841 gatgtcgccg acgatggcga ttgtcaaatt gtttggcacg taataggtgt cgaaaaattg
    12901 tttcgcctcg gagacattaa gatactccag gtcgctgggc cagccgaccg gcggctggcg
    12961 gtaggggtgg gcaatgaagg cttggcccag gaaggtctcg accagtttgc cgacgggatt
    13021 cgagtcggtg cgcatgcgcc gctcctcccg taccacgtcg cgctctttgt aaaactctcg
    13081 aaacaccgga tgcaggaagc gctccgattc catgtagaac caaagctccg cgcggttcga
    13141 gggcaggctg taaaagtaga cggtggaatc cggccccgtg gcggcgttca tgcccacgcc
    13201 gccgttctcc tcgatgatgc ggctgtaatc attggattcc gccagttcgc ccgctttttc
    13261 caccgccgct tgaaaagcct tctccagctg ttctactttg gccctgtccg ccagcctccc
    13321 tttgtttttc tcggccatca aatcggaaaa tgcctgatcc acagcattga gagccttttt
    13381 ttcggcgggc cagtttttgg acccgatccg ctccgtgccc ttgaaagcca tatgctcgaa
    13441 catatgggca aggcccgttg cgcctctcgg atcatccact gatcccacat tggcataggt
    13501 gtaaaaagag acgacaggag cctcgtggcg ttcgacggcg ataaagtgca aaccgttggg
    13561 cagagtgaat tctgtaacgg acttctcgaa ctccttcaga ttttgcgggc gcgccggcag
    13621 gaccagcagc cccgccaaaa acaagtagcg cagtcccgaa gctcggctgg caaacatatt
    13681 tctaagttat cacagggcgg ccgggggctc gggagccgcc gggtgccgtc cccgtactcc
    13741 gccaaaggcg cattcgttgg cgaaacggga acgtcccgct ggccacgctt gcttgtcccc
    13801 gcttccttct ttgagaagat ccgctgcggc ggccgtggag cgcggcggaa acggcggtta
    13861 ggaactgcgc gcggatgcgc tacactgtac gtgttgcgac gcctcgccgc ctttctggcc
    13921 cccactggaa agtttctcct gcaaacggaa attcacgctt acgcctttgc catctcggcc
    13981 aaccttctgc tgtcgttctt cccgtttctc gtcctgatgc tctccatttg ccagaatctg
    14041 ctgcactggc cccgagcggc cgatgttatc tatctcgcgc ttcaggattt tctgccgtcc
    14101 gatccgggcg ttttggactt tgtccgccgc aatctgcgag cggcggtatc cgcgcatggg
    14161 cgcatcgctg tgttttccgc gatcctgctt ctggccacaa gcaacggcgt ctttgagccc
    14221 ctcgaggtgg cgctgaatcg ggtatgggga atcggctcga accgcagctt ttggcgcaat
    14281 cagtgggtaa gctttggatt gattctcgtt tgcggcgccc tggcgttcat ctccattctc
    14341 tttacgacga tgaaccggga gttgctggat gctctggccg ggccgctgcc cgccttggcc
    14401 cgctgggcga gcctcgcggc gtttaaagcg gccgcgctgc ccgtctcggt tgcgattctc
    14461 ttcctcgtat attggctgct tcccaacggc ttgattccgt ggcgcagcgc cctgacggcg
    14521 gcgattgtga tcggcgtggt catggaagcg gccaagtatc tctacattct ggtctggccg
    14581 ctcctcgact tccggcgcgt ctatgggccg ttcttcattt ctgtcaccgt gatcgtgtgg
    14641 ggcgtggccg cttcgctgat cttgctcgcc ggcgcggaaa tctccgcgcg tttacagcgc
    14701 gagcgcgccg gccgggctgc ggccggccaa gcctttcctc agaaacagat tcctaaaaat
    14761 ttgttttgac tttggccgct ttcgtgctaa aacattcact ggacggattg tagtgggcgg
    14821 cgctagcggg atttctccaa ccgctcctta caatccgtcc agggaagtta ctgggcctca
    14881 gcagttcaat ttcaaccacg aggtgataca tggcgaagaa accggcaaag aaagccgcca
    14941 agaagccaaa gaagaagtaa ctggatttta cggccatctc aactgcaagt ttcaggcagc
    15001 ctgctcggcg ggctgccttt tttatcgccc tttgctgggc gggaaagccc gccaagatcc
    15061 ggccgcaaac atcggttgaa gtttcgccgc tccgggcttc aagtctacgc gcgcaatcgc
    15121 gctacggccg cctgaatcgc ctgctggacg tgcaggcgct cctcacggtc tttgtaagcc
    15181 tgtctgggcc agaagaatcc tttcaatccg tccttcctcc gcctgggaac aatatggata
    15241 tgcagatggg gcacactctg gcttacgcgg ttattgatgg cgacaaagga gccttcggcc
    15301 tccaaaccca gctccattgc ccggctgagc aactgggcgc cggaaaatag aggcccgagc
    15361 agattgccgg gcaaatccgc cagagtctcg tagtgttgct tgggcagcaa aagagaatgt
    15421 cccgggaata acggcctgtg gtcgagaaac gccaggcaaa ccgaatcttc aaacacccgg
    15481 tacgcttccg ccttcccgag aataatgcgg cagaagaggc acgtttcggg aaaggaacgc
    15541 tgggaatcgg cgccgctgcc cgcggagaga ttgggggaat ccagtttcaa cgccgtcggt
    15601 tgatgcagac ggcgagtata tcatttcttg cttaagaagc cgctgctcga gaacgtcccg
    15661 ggttcaaccc ggaagctggc cgtgggcttg aattcgcccg cagccggctt gaagtctccc
    15721 ggcggcacag agaagctccc cggcgctggg tggaattcct ttgagggttg gaacgtcccg
    15781 gccggaacgc tgaagctgcc ggatgcgggc tcgaaacttt cggagggctt gaatgttccc
    15841 ccggtgactg agaaaactcc gtgcggtccg gcaaagcttc ccgtctgttg gtcagcgtgg
    15901 cctgcgggcg gccaaaggca cacgaatacg gcaagcacga aaccgagatt gcgcatattc
    15961 tttctccttc acccgcccaa tcgtcagaac aggagaaaag ctttagagag gtcgaaatcg
    16021 attggtttgt tggctctggc cggctgtgca ggaaaatatc ccggagggaa gaatcccgcc
    16081 ggcaaggcca cctggcgaag aacaggggaa ccatccgttt ggatcttttt gcggcggcag
    16141 ccgcgatgca aagtgcaata ggggagaatg ggcgaatcgg cttgccctgc acagcggact
    16201 gcctggcgcg gtacattcct attggagtgc ggcgatggcg tctgggctgc ttccgtcgta
    16261 gccgggcgcc ggcgccagga gatagccggc cacggaattc ttggtgacat gcgtgatcac
    16321 gagctcgcga ggcctgttgt cgtcctttgt gtagaagatc actggccgct gggcgctctg
    16381 ttgccggacc cagacggtgc ggcggtcggg cagcagccag atccagccat cgaactttcg
    16441 atagcccaca tcaatccggg taacctcgat cgtcaccccg ggcgcaacct cccggctgtg
    16501 atttttggcg atctcaaaat caatgcgttt tctctccacc ttggctgaca gggagcccaa
    16561 gtttcttcgc tctcggtccg catcgcggtc cagacgcgcg acctgccggc tcactccgtc
    16621 tagttgccgg ccggtttcct gttgcaggaa agcgatctgc tgcgactgcc gcgcgatctg
    16681 ctctcgggcc gcggagactt cgttttgcaa ttgcacaaga cgcgtctgct cctccccctg
    16741 gctcgcttct acaacgccca ggcgcgcctg caacgcgtcc atgcgtcctg catgctcagc
    16801 ctctatgtgc tgctgggcct gggcgacgat ctcctgggtt tgtttgcgcg ccgtttgcag
    16861 gtttgcagta actctgctct cgagcctggt cacgcgggcg gcgacgccgt cttgatcggc
    16921 cgacaaggct gccagccgat cctcggctgc gcggattctt tgctgcgccg cggcggcaga
    16981 ttgctgcaat ccgggaagac gcgcgagaag gtcagcctgt tttcgaatct ccgggaagct
    17041 atatctgtag gcgccgacgc ccagcaaagc ggcaatcgac gcccaagcag ctccgcgaat
    17101 aaattttttt ctgcggctcg ggttctcggc aaaaatcagc ggcggcaaac cagggttgcg
    17161 ggatggttgc atggcgcctc ctcagaactg ctggctagat ccgtgtgctg tatcgcacca
    17221 gaccggtgca atcccgtttc catcggtcct ggaggcagtt tttgaacagg ttaggcggcg
    17281 tcctgtgcgt aatttctgct cgcaagggcg gctaaatttt tccgctgcct gttttttcgc
    17341 tggacaaggg cggcaagagc agaaacgcag gaaggcgact gcaatctctc cacgtcagac
    17401 cagcacagtg aaagtagctt cgttcctgct caccggccgg tacaaagtgt cgcgctcaac
    17461 gggatcccgg cccgcgcggc gaatcaggtg caacagttcg ctgcgccgca gggactgcgc
    17521 cgtggtcgct ccggcatcgt ggtaaatctt ctcttcgaca accgtgccgt cgatgtcatc
    17581 cgcgccgaag cgctgcgcga cctgcgcgat cttcggagtc atcatgatcc agtaggcttt
    17641 gatgtgcggg aaattgtcga gcagcagccg ggcgatagcg acattcttga tgtcggcgaa
    17701 accagtggtt ggcgagatgt gttgcagcgg cgtgttggcc ggatggaacg ccagcggaat
    17761 aaacgttaca aatccccggg tctcatcctg caaagcccgc aacttgagca ggtgatcgac
    17821 ccggtcctcg tctgtttcga tatgcccgta aagcattgtg gcgttcgaat gcaagccgat
    17881 gcgatgcgcc gttcgcgccg tctccagcca ctcctcgccg ctgattttgt gatcgcagat
    17941 aatccgccgc acgcgctcta caaaaatttc ggcgcccccg ccgggcatcg aatctacgcc
    18001 ggcttgcttg agccgccgca aggtttcctc gatcgaaaga cgcgaccgcc gcgccagata
    18061 gccggcctcg accatcgtaa acgcctttaa atgcacctgc ggaaagcgct gcttgaggcc
    18121 ggcgatcatt tcgcaatacc aatcgagagt cagttccggg tgcaggccgc ccacgatgtg
    18181 aaactcgctc accgcttcgg tatagcccga tccggcggtg tgccagactt cctcgagcga
    18241 catcgtgtaa gctttgggat cgcgcacctg ccgcccgaaa gcgcataact tgcacgccgc
    18301 cacgcacacg ttggtcggat tgatgtgccg gttgacgttg aagtaagtta cattgccgct
    18361 cttgcgctcc cgcaccaggt tcgcaagata cccgatggtc agaatatcgt gggagcgata
    18421 cagggtgacg ccatccttat aggtgaggcg ctccgaggcc agcacctttt ccgcgatcgg
    18481 cttcaggctt gggtcgtcga tgacggaggg ttcaagcagc ggcatagaga atatcagcgg
    18541 cccaaaacaa aaagaataac acgctgacca cgccgttcac agtgaaaaac gccgcattca
    18601 cgcgcgagag atcaccggga cggactaagc tgtgttcgta aatcaatagc gccgtcacca
    18661 gcccgacgcc tgccaggctc agcggcccta gcgacagggc ttccgcgagc cagacgagca
    18721 gaacgaccat cgcggcgtgc aggagccggg cgacccacaa agccggtgca atgcccaggc
    18781 gcgcgggcat actgtgcagc cgctcgcgcc ggtcaaagtc gtaatcctgg cacgaataga
    18841 taatgtcgaa gcccgccgtc cacaacatca ccgccgccgt caggagcaaa ataggagcat
    18901 ccaggcgccc acgtacggcg atccaagccg ctgctggcgc tatgcccagg caaaatccca
    18961 gaatcagatg ggaaaaggct gtaaaacgct tggtatagga gtagaagaac acgatgccca
    19021 gagccagcgg cgccagcttc aagcacagcg cgttgagttt gccggcggcg agcaggaatg
    19081 tagcgctggc tacggcgaca aaacccgcag taaaggaccg cgacagcacg cctgacggta
    19141 tggctcgcat ccgggtccgc ggattgcggg cgtcgatctg cgcgtcggcc aggcgattga
    19201 aagccatggc cgccgaccgc gccgacacca tggccagaac gacccaaccc agcttgcccc
    19261 ctactgccgc caggctgagc ttctcctcgc gagctgccag caaagcgccg gttagggcga
    19321 acggcagcgc gaacaccgaa tgctcgaact tgatcatttc gagcgtgatg cggagtttgc
    19381 ggagcgctcg cattcccggt tcatcatagc aggcaatctt gtagaatcac tgcggaggaa
    19441 gcggcatgaa aatccaatcc cttgtgatcg gagcagcggc aatgctagct gccgcggcgc
    19501 tcggcttgct ctccggcggc cgcgcttttt ccagcccgca agccggttca gggcaaagac
    19561 agccggcgtc cggctcgttg atcgagggct tccgtcttgt cgaggtggcc tctgtcgccg
    19621 acgccatgga gcaactttac ggcgagcgga cttatatgag tcaccagatg cggcctctgt
    19681 ttccaacgaa gttcgccggt cctgcggtga ccgtgctgat gaagaaggag gagcacaagg
    19741 agggggctcc ggcttcccag ggcatgctcg atgcgatcga tgccgcgcct ccgggctcgg
    19801 tctatgtcat ggtaatgcct gagggcgccg acttcgccgg catgggcggg ctgatggcca
    19861 cggcaatgaa attccgaggc ttggccgggg cgatcattga cgccggaatc cgcgatacgc
    19921 cccaaatcag aaagctccag ttcccggtct tcagccgggg cgtggttccc tctacctcga
    19981 tcaaccatta ccgtttcgcc ggcgccaacg tgccggtgaa ctgcgcgggg gtgcaagtaa
    20041 atccgagcga catcatcgtg gccgacgagg acggcgtggc cgtggtgccc cgcacgcgcg
    20101 ccgccgccgt gttgcggcga gcccaggagc tcgatgatac cgagcaccgc atgctgcctt
    20161 tcatcgagaa attccgttcg ataaaagaag cagtcgcaaa gttcggaaga atttgagccc
    20221 gctcatcatc ttgcgccttc ggcggccccg agcctgcccc ccgccggatc aggacaccgg
    20281 agctaccggg gagaggcgtc cggaaaagcc agcatctgtt cggcggcggc caggtcctcg
    20341 gggcggccca catcgcgcca ccagccggga atcgggtagt agccgacggg cagacccttg
    20401 gcaagcaggg ttcgaatgcc gtcagtcagt tcgtattcgc cgcgcgggga gacggagacg
    20461 ccggccaact gctcaaagat ggaagggcga aagcagtaga agccggcgct gttccaatgg
    20521 gtagctgagg taccgcgggg tggcttttcg atgatgtcgt ttacgcggtc gccctcgatg
    20581 tacacggctg cgccttgata cggatcatcc acatatttca ccgcaagcac tgcctcaaaa
    20641 ccgtccatcc ggttcgcagt ctcccggtag gcctctggcg cgaccaaaat atcgccgtag
    20701 gtgagtaaga aaggctcctc gcgggcgaag tcccgggcga gctcggcggc tcgtgcggtg
    20761 ccatcgggaa cttcctggcg gcagaaactc acgccgggtt ctcggacgaa atacgcctcg
    20821 atctgctccg cgcaatagcc cgttacgatg aggatgtgcc gcaacccagc ggtcttgagg
    20881 ttgataatca agtgggccaa aatgggcttg ttccggacct ccagcatcgg cttgggcacg
    20941 ccggcagtga gggtttgcat ccggctgccg cggcctgccg caagaataac ggctcttgtg
    21001 acgtgcacgt tttgattcta acgccccgcg ctgccgccgt cccgtggcga tgtccggccg
    21061 cagctcttcc ctgaagcatc gcaagtgtct aaattggcgg ggccctgcgc cgctggttct
    21121 caaactaact ctacgcccgg gatttgaggt aaaattgcgt ttgaggcctg ttatgagatt
    21181 ttgccgcaga attatagtcg ttgctctgtg ttgcgcggcg gctgtggcca tggccgcgac
    21241 ttctccatcg ggcgaggtgg tgctgcatgg gaagatccgg caggaagcgg gcaagcctcc
    21301 ggtgatcgaa accgcggatc atcaggtcta cacagtttca ggtgatgaat ttctcacggc
    21361 ccaaatggct gactcgcggc tgaacggccg cgacatcgtt ctcgagggcc atgtgaccgg
    21421 ccccggtaaa tttgaggctt ctcatctcta cacgattaag aatgggaagc gctacgaagt
    21481 gacctattgg tgcgacgtct gttccattcg cacgcacatg cccggccggt gcatgtgctg
    21541 ccaggggcct acggaactgc aagagctgcc ggtcccctga cctgccactc aaccgttgtt
    21601 tccgtagctg cgtgtttctc cagcttcagc cagcattcgc tttacccgat cgccgccact
    21661 cggattcccg cggggcgtcg ccacacactt gctgagattg gtttgccgcg caggcgagcc
    21721 gaaacagccg aacctcgcta ttttgcccga tcagggagcc gaccaggtga cctttccgtc
    21781 ggcgttgtaa aaggtgatgg atgggttccc gcttgccgag accgacatcc ggccgcgcgg
    21841 ctgcccggct ggatcgagca gcgtaacgcc atcggaaaat acggagacgg gcctgccatc
    21901 cttgtcccag aattgcaaac cgggtgtgcc gttggtggcg acgagaaggc ccgtccggat
    21961 tttgccatcg ttatcgtaaa agacgagccg cacagctccg ttgggcagag aatcgaaccg
    22021 gccgcgcctc ttgccttctt cgtcgattac ctcgaagcgt tgggcgcgaa gcaccttgga
    22081 attttcctct gttgcctggg caaaagcggg ccgcgcgacc aggagtcggc tgacgaaggc
    22141 gccgcccgct actccgccgg cgacagccaa tagcaaaacg aatatgtact gctttctaga
    22201 catctgcggc tcctctgctt gcgctcccta acctgcggag ctttcttaac gcgacctcag
    22261 cctgcaaatc attgccgggt ggaattttcg tccctaagca ggccggttgt gcgggattgc
    22321 ccgcttgcgg ctcagtttac ggccgccgag cggatcgtag gccggcgttt gaacgaatag
    22381 cgccggcggc gcgattagcc ccgattttcg aaggcgcact ggagcgcgcg gtttcatgat
    22441 cctgaatcgg cgactttcct cttcggtctc cgattggttt cgagaatctt tttgcgcagc
    22501 cgaatcgatt gcggcgtgac ttcgaccagt tcatcctcat tgacgaattc aatcgcctgc
    22561 tcgagattga gcgtgcggaa ggggatcagc cggatggctt catcggccac cgacgcccgc
    22621 atgttggtga gctttttttc tttgaccacg ttcacgtcga ggtcggccgc gcgcgagttt
    22681 tccccgatga tcatcccttc gtaaacctcg acgccggggc ccacaaacag ctcaccgcgt
    22741 tcctgaagat tatagagggc gtatccggtg gtacggcccg gccggtcggc gaccaaggcg
    22801 cctgtagggc gcatcgggat ctcgccctgc cagggcgtgt agccttcgaa aagggaatgc
    22861 atcacggcgg tgccgcgggt atcggtcatc agttgactgc gcaggccgat gagtccgcgc
    22921 gagggaatcg taaattccat tcgcacccgg ccgctgccgt tgttgaccat ccgggtcatc
    22981 tggcctttgc ggatgccaag catctcgatg accagtccgg cgaactcttc ggcgcaatcg
    23041 acgactaaag tctccaccgg ctcgctcaac acaccgccga tcagccgggt gagaatctgc
    23101 ggcttgccca cggccagttc gtagccttcc cgccgcatgg tttcgatcag gatggccagt
    23161 tgcagttcgc cgcggcccat gacctggaag cagtcggtcg aactcatcgg ctcgacgcga
    23221 atcgaaacat tggtcagcag ctccttatcc agccgctcgc gcaagtgccg ggatgtgaca
    23281 aagcgacctt cgcgccccga aaaaggcgag gtgttgacgg tgaataccat gccgatcgtc
    23341 ggctcatcga tgtggagcgg ttcgaggggc gccggagtct cggccgaagt aatcgtctcg
    23401 ccgatcgtga tgccttctat gccagcgatc gcaacaatat cgccggtgcg ggcggtttca
    23461 atctcagttc gcttcaatcc ttcaaagcta aacagcttgg taatgcgcgt cttctgcacg
    23521 cttccgtctc gcttggcgat tcccacctct tcgcccgcgc gcagctcgcc ctgaaacacc
    23581 ctgccgatcg cgatgcgccc caggtagtcg ctgtaatcga ggttggcgac cagcaattgc
    23641 aacctgccct ggggttccgc cgagggcggc ggaatcgagt gcagaatggt ctcgaacaag
    23701 ggttgcaggt cggagcccgg ttcggctgcg cttagcccgg cccggccggt ccgggcgttg
    23761 caataaagca ccggaaattc aagctggtcc tcggtcgctc
//
LOCUS       DASREK010000222        21861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2414507, whole genome shotgun sequence.
ACCESSION   DASREK010000222 DASREK010000000
VERSION     DASREK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 21861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21861
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414507"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFA54_11605"
     CDS             complement(<1..466)
                     /locus_tag="VFA54_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZS51501.1"
                     /translation="MIFALLPVKPPDRAKQRLSRVLEQRFRETLARAMYEHVLSVLCA
                     SSGLDRIAVMTADQGAADQARRSGAMVFDEPELLGHKKSADLAARRAMCYGARTVIMV
                     PIDVPLIAPADIEELTAAARAAAGPCAFIVPSWDRSGTNALVLTPPDAMQTCF"
     gene            complement(463..1389)
                     /gene="cofD"
                     /locus_tag="VFA54_11610"
     CDS             complement(463..1389)
                     /gene="cofD"
                     /locus_tag="VFA54_11610"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HZS51502.1"
                     /translation="MKKIVALAGGVGAARFLDGLVRLIQPSRLVIIGNTGDDAEIHGL
                     RVCPDLDTVTYTLAGLADPERGWGLAAETWRCQEALKRLGGETWFQLGDSDLATHLYR
                     SERLCKGAALSQVTAQIARRLGVKAAIVPMSDDRVRTKVRTALGELEFQTYFVRRRAR
                     DRVLGVQFEGAARAAPAPGVIEAIKEARAVILCPSNPIISIGPILAVPGIRETLRQTH
                     APVAAVSPIVGGRALKGPAARMMKSMGLRASALQVAELYRDIVDVFVLDWVDCAQAAA
                     IEKLGMRAVVTGTIMKDPAAKRALAKTVLEAL"
     gene            complement(1386..2036)
                     /gene="npdG"
                     /locus_tag="VFA54_11615"
     CDS             complement(1386..2036)
                     /gene="npdG"
                     /locus_tag="VFA54_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HZS51503.1"
                     /translation="MRTIAIVGGTGAEGFGLALRFAAAGARVFIGSRDKSRAQAAAAR
                     IPGAEGRLNPDAVREAEIVILTVPLAAQISTLKSLRQSFRSGAILVDATVPLEAAIGG
                     RVSRTLGLWAGSAAQQASQHIPEGVSVVAAFHSLSAELLQAAGRPIESDVLLCGDNAE
                     AKKAVCELIELLPEARAIDAGPLENARLLESLAALLISLNIRHKAKHAGVRITGLE"
     gene            complement(2073..3263)
                     /locus_tag="VFA54_11620"
     CDS             complement(2073..3263)
                     /locus_tag="VFA54_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZS51504.1"
                     /translation="MKRRTSLGMRRRKSAVVVGAGAFGGWTALHLLRQGVWVMLVDAW
                     GPGNSRASSGGETRILRATYGPDRVYVEMARRSLDLWRAHEEKWSRRLYHQISVLWMA
                     PQDDHYERAALPVLRETGVDVEELTAKEAAARYPQINFEGVKWAILERDAGYLSAREA
                     CRAVLEGFLVEGGEYRQLLARPGAIRGGEMQEVVLSDGSSLVADAYVFACGPWLGQLF
                     PQAVGDRIVPTRQEVFFFGAPAGDDRFSEGRLPVWIDHGQPMMYGIPNARGFKLADDT
                     RGDCFDPTGGDRVVRTESIAAARKYLAFRFPALKDAPLIESRVCQYENSPDHDFIIDR
                     HPQAGNVWIAGGGSGHAFKHGPAVGERVADLVLERAKVDPLFGLARFSPGFSGAAAPH
                     LNHV"
     gene            3408..3905
                     /locus_tag="VFA54_11625"
     CDS             3408..3905
                     /locus_tag="VFA54_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS51505.1"
                     /translation="MAGSLTTRIRRLTRADHQALLGMYRRFEPLGVAAGLPPRNEQAR
                     REWIERSLEHEINFGAFSPNGEMAGHCFLAADGPGSAEMAVFVHQDFRNQGMGRQLIN
                     AAIQEACRKHIQRIWTMTSADNLAALAVLKRCGFQARRGPVTAFDLELELEPSCAGTA
                     PEWRN"
     gene            complement(4023..6791)
                     /locus_tag="VFA54_11630"
     CDS             complement(4023..6791)
                     /locus_tag="VFA54_11630"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSD1 and planctomycete cytochrome C
                     domain-containing protein"
                     /protein_id="HZS51506.1"
                     /translation="MHTRRVLCTDVRGCLFALLIPFFLFSLNAAGQTPSISFSRDVAP
                     ILAQCMQCHGSATLSANLDLRTPEGAKKGGMHGPVIIPGDAEHSHVYRRITAQEDPRM
                     PYNGEPLTDKEIKIIKDWIDSGAQWDKSVDMVALYNSLPKKPANLDRVITAKDREWWA
                     FQRPVRYPVPEVNDPRWSANPIDAFLKKQMDAKGLTPAPMADRRTLIRRAYLDLTGLL
                     PPPEEVDAFVNDKSPDAWGKLIDRLLASEHYGERWARHWLDVARYADSSGYEHDRDFE
                     NAWRYRDYVIKAFNEDKPYNQFIIEQLAGDEVDHPTYDSLIATTFNRVGPRVRFREKD
                     NPYYRYEYLDDMIGTTSRGFLGLTVNCARCHDHKFDPIGRMDYYRMMAMFFPFVNYDF
                     PLAPPDQAAAYEKKVKEIQDQITPLRIEIARIEKPYKEKRFEEMLKSYPEDIQVAVHT
                     PEDKRTPGQKLIAAQFLHGLEDPDAAAAAAFNGAAYRRRLMKLNDEDTAKRQVLLDKI
                     AALEKQMPPPIPIAEGIRDGDYRFTPDGPGDEPLPGKGNRPNWGTGKYIPDPGDNYEP
                     PKVHFGANGQVVADDEKAPVVEPGFIKVLIDPDNPPPVSIKPADGRVTSGRRRALAEW
                     IASEKNPLTARVMVNRIWQHHFGKGIVSTPSNYGKLGSPPSNPQLLDWLATEFVRQGW
                     SIKKMHRLIMTSQAYQMASAFYDAEDLRKDPTDTYLWRFPLNRLEGDIIRDIILSASG
                     QLNLQAGGPPFFPSIPKSVREGFRQGKWVLTKEEPSTWRRSIYSYWKRGMKYPMFDVL
                     DEPDVNVTCEARTVTTVPTQALTLMNNEFVLLQAKYFAQRVEQEAGADKAAQVKRAYN
                     IALSRDPTPSELQGNLKFLEKQLAYHQSKKAQQTDESAAVKSDPETAALTDLCDVILN
                     LNEFVYIN"
     gene            7062..7955
                     /locus_tag="VFA54_11635"
     CDS             7062..7955
                     /locus_tag="VFA54_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZS51507.1"
                     /translation="MDAGQIRRIAVIGAGQMGRQIAMLAALGGYAAALHDISEVQMER
                     AFAENRRRLEKQVLRGKLAAEEMEAALGRLRPAMTLEEAAGAADFVIEAVVERLEEKR
                     KVFANLDSICPAHAILATNSSYIGNSRLAAVTRRPEKVINMHFFFPPLVMRLVEVVKG
                     EKTSEETVGVTADLARRMGREPVVLRKELPGFLVNRILRAIQSEAYYLLENGVAGFAD
                     IDKAVELGLNFPMGPFKLADFSGLDVGYYSRMDRYRETGNPAEQPPAALRIRVERGDL
                     GRKTGRGFYDYSVDPPAPTED"
     gene            8516..9226
                     /locus_tag="VFA54_11640"
     CDS             8516..9226
                     /locus_tag="VFA54_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS51508.1"
                     /translation="MPASLARVLLVDDDAQFFEEVKSRLRTERDIIIGWSRTRALEDV
                     PDDEYAHADVVMTDSDKHGPELADTVSRVKSRFHHLQFLILGSAEDDTVTMILLESGA
                     RGYLDLQTSGENLASAIRRLASGGTWVPPHILARYMDLIQMVEFLPKTGAENDPWAQF
                     TAREQRVLTLMACGRSNKEIGRVIGVDETIVSSTIHQLMHRTGAVNRPSLIAYYLYHK
                     LFRGESLPAMDAEKVLEN"
     gene            complement(9236..10108)
                     /locus_tag="VFA54_11645"
     CDS             complement(9236..10108)
                     /locus_tag="VFA54_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HZS51509.1"
                     /translation="MHTNVGDQKMRRREALKLAVLGAGGAMSAAGQNWAMKPPAPAFH
                     GLKIGLTSYTTRKLTYERTLEELKGIRIEYISLKDVHLPLSSTREERLAAKKKAEDAG
                     VKIMGVGVIYLKNDEAQIRHALQYAKDLGVPVATISPERAALPAVEKAVKEMDIQVAI
                     HNHGPRDKVWPSPLGVFDAVRGLDSRIGCCVDVGHTFLLGVDPVDALRKCAPRLYGVH
                     LKDLAAPQGPQDVEPHNVPVGTGVLDVVAMLRVLREIHFTQLAALEYEAEAEHPVRGM
                     AESFGYLRGVLAAI"
     gene            complement(10122..10817)
                     /gene="eda"
                     /locus_tag="VFA54_11650"
     CDS             complement(10122..10817)
                     /gene="eda"
                     /locus_tag="VFA54_11650"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZS51510.1"
                     /translation="MAKSKTLSFILDIGVVPIVRASSPEAAIQAVGAIYRGGIRAVEI
                     TMTVPGALRVLEQVADQFGDRLTLGAGTVLDAESARACMLAGAEFFVTPTLRLPTIEI
                     CHRYSKVVIPGALTPTEVLTAWDAGADLVKVFPCGNVGGPKYIRALKAPLPQVEMVPT
                     GGVNLENTADFLRAGASAVAVGSEMVNAQALAMGNLEVVEENARRFLAVVAEARRSMK
                     LHETRDLPTVTRA"
     gene            10863..11684
                     /locus_tag="VFA54_11655"
     CDS             10863..11684
                     /locus_tag="VFA54_11655"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HZS51511.1"
                     /translation="MQPNLLAKSPLQEPGVYPIVKRGSGLKHLSFTVVQMGPALREYS
                     AESGEEELALDFYPGQVRVEAQSPQGNIERTTRLRRSLKEVGEMLYLPSGSRFRITLL
                     SDNARVGIAGSLGKPGVPPVFISQDQVMSKKVGKPSWLRTVHTHIGDNVNAAHLIVGE
                     TVNDPGNWSSSPPHKHDRHAPPAEVPMEEVYFFQVEPRGGFGFMRVYTDPADPEPFDC
                     VYTVEDGDTVLIPKGYHPVAAFPGYTLNYTWALAGEGRVYGAWTDDPRHTWIKDN"
     gene            11709..12134
                     /locus_tag="VFA54_11660"
     CDS             11709..12134
                     /locus_tag="VFA54_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51512.1"
                     /translation="MERKYRQRGYQDDDSERRAEKPSRPPGPRETIGPRLPQFPARVT
                     VARCANCGTLLPKDFDPKGQCPRCGFELHCCKQCVHFDTSARWECTQPIPARIARKDA
                     RNDCAFYEIRMSSERETTSGRGPSRPDDPRQAFENLFKK"
     gene            12377..13858
                     /locus_tag="VFA54_11665"
     CDS             12377..13858
                     /locus_tag="VFA54_11665"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZS51513.1"
                     /translation="MKAAHLRALLFAGAALAAGASGCAHKAPVEPPKPPLVPAVFQPL
                     QDALQKSYLELFQLAPTLEFSNTQIQNMRTYLEQARRYCVGQYKKKSDQYSADLKRLE
                     GNLKQVSATASDTQRHDLHCQIQNTRALQSQTEVLADHAIPVAYENKEAKLDLIQQWP
                     GDLKQIKQQIADGSYLKRQWGDVKDIGFREIASGQKDDIKTGEDAIKQMKQAGLMPKE
                     LDNKAITEYVEDVAQKVAKHSDLQIPLHVTILNSKEINAFALPGGFLFVERGLLDAAD
                     DESELAGVIGHEIGHVVARHGHKLMQKATIESVVFQAAQVAAVLLTGGAAGIGTYYAL
                     QYGFYGLGLALNLNLLGVSREYELQADQLGIQYAWNSGYDPSGFIRFFDKMATQEGYV
                     NGASWFRDHPPFYQRMLDAEREIMFLPKKENLVVQSSAFEQMKKELKPVTAKAEAEEK
                     GRPSLLAPEQGCPAPQKIEYKPGEPIETICSLPGTVTTKVASK"
     gene            13813..14766
                     /locus_tag="VFA54_11670"
     CDS             13813..14766
                     /locus_tag="VFA54_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS51514.1"
                     /translation="MFAARDSDHKGGKQITALAGLAFGAILVAFLARPVTAQFPRLVS
                     LYATVERDGGLVRGLGPQNFHLYEDGDSRQFHLEPPETPMVIALLVEYSQRSYPYFND
                     IQTAVESFVRQAPEGNWYALATFSHNLEVQVDFTKQVMQVSNAFSQLGEPMWNEVDTY
                     DAVYEMLDKMERLPGRRVLIVIASGLDSFSSHTLDDVQKKIDAVNVVIYGVGAGSMLR
                     GIYEPYLGTMQEMSLLQAQTFLQMLADKSGGEAWFPKLEGAFPDVIEGIFQDLENQYR
                     LVYESHVPADHKLHKIKLEAFQIINDKRQNFKVRVREGWRF"
     gene            15010..15699
                     /locus_tag="VFA54_11675"
     CDS             15010..15699
                     /locus_tag="VFA54_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51515.1"
                     /translation="MADRHPILRLEQVTKDYLSNGAAVRALDAVSLEVRRGEFVALVG
                     RSGCGKTTLLNMAGAMDFPTSGRVWLDGACTSELNDAGLTALRRSKIGFVFQFFQLLP
                     TLSAVENVELPLLLAGRSGARLQALERLQWVEMQTLAHRLPHQLSGGQMQRVAIARAL
                     VHNPQILLADEPIGNLDTASGEVVLKLLRRAASELKSSVIMATHSAEAAAAADTVVRL
                     RDGRIAEIRRV"
     gene            15696..18221
                     /locus_tag="VFA54_11680"
     CDS             15696..18221
                     /locus_tag="VFA54_11680"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZS51516.1"
                     /translation="MTSRWRLFHRLILRPLVHERLRTALTVFAVALGVAVVVAIDLAG
                     AAAAGSFRSSLESLAGRTDLDLSAISGIDERVFVRLSELPFPAQFRPYIDDFARVEST
                     GVTVPLAGLDLLGESDWSLGGDWGSGWNDPPDLSSSVWVGPRIGAAAGSKIRLTLNDR
                     SREYTVRGVLKLQGLARYTQDDLIVMDISAAQEALGKVGRLDRIAVTLPRGADLAEWE
                     QRLRGVVPADVSIERPGARSEENQKMLSAFRWNLRVLSYISLVVGAFLIYNTVGVSVV
                     RRRTEIGVWRALGATRGVVLGAFLAEAAFFGLTGSLTGLLLGRLMAEGAVRLLASTVQ
                     SLYVSSRPAPIEFTPATMLLGAATGLGVALLSALAPAREATNISPIEAMARGLREHQA
                     RVSWRRDLLAGAAVTLGAAAASRLPPIQGKPVFGYVATFLLIAASALATPALVLGVSR
                     LVRMSLGVEPALAARSLAASLNRTSVMVGALATAVAMMASVGIMVGSFRQTVILWMNT
                     QLRADLYVRPAGRNGAGQHPTLDSRLADRIEQLPGVDAVDRFRAYEISFRGLPCTLAG
                     GESAIVRRFGRLRFLSGEDRNRILDELPRGDYALASEPLANKHGLRPGDTITLPLAGA
                     PRRFQILGVYYDYSSERGLIILDRSVLLKYLPDPAPSNLAVYVRRGVDPEQVRRAIEA
                     AAAGHTVLIEPNSRLRAEAIRTFDRTFAITYALEAVAILVAVMGIAGALLALVIDRRR
                     ELGLLRFLGASAVQIRRLLFFEAALLGLLANALGLALGGVLSLILIFVINKQSFGWTI
                     QFHWPAALLAAGLTLVYAATVLAAWLPARAAARLKPIEVVHEE"
     gene            18218..19288
                     /locus_tag="VFA54_11685"
     CDS             18218..19288
                     /locus_tag="VFA54_11685"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HZS51517.1"
                     /translation="MKHFLWIAALVFQIARPGYRYEFPRDHFNHPGFQTEWWYYTGNL
                     QTPEGRPFGFELTFFRRAVAGRENRPAHARPDSTWELDDVYLAHLALSDIRGRRFYAA
                     ERLNRAGPGLAGASLEQRRIWNGNWQARWNGARQSLQGVAEDFSFELELDPQKPPVIH
                     GRDGISQKANGEGRASHYISFTRLAAAGAITLRGVRFPVSGAAWMDHEFFTHQLDASQ
                     AGWDWFSIQVDNGTELMLFRLRRKDGTIDPYSAGTFVDRQGRAQHLEFHDFALEPLET
                     WTSPATGARYPVHWKIQVPPLGIDLDCSTALPFQELVDRRQPELNYWEGAVTYHGRSG
                     DKAASGVGYLEMTGYGHPLRMD"
     gene            19263..20390
                     /locus_tag="VFA54_11690"
     CDS             19263..20390
                     /locus_tag="VFA54_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZS51518.1"
                     /translation="MATRSGWISARNSPGPQFALCYNARELAMPIARLRRRRQFLQCA
                     AGAVLFACRDGTIAAPGSMKTALLADAIYKEHVTGPGHPERPERCDAVTEALHKGGLM
                     PSLDRIGVRPATEEEIGACHTRAYIELVKREIMAGASELSTGDTNVGPRSLEVALRAA
                     GGVLNAVDAVISRKARNAFCAVRPPGHHARPAQGMGFCIFNNVAIAARYAQRKHHVEK
                     VLIADWDVHHGNGTQDIFYADGSVFFFSTHQSPWYPFTGRADETGEGNGAGCTMNCPF
                     PAGSGREEIVGAFRRKLAPAAQNFRPDLVLISAGFDSRADDPLGRFTLSDEDFAELTA
                     IMLEIADKHAGGRLVSVLEGGYNLPGLASAALSHVKALTSA"
     gene            complement(20479..20994)
                     /locus_tag="VFA54_11695"
     CDS             complement(20479..20994)
                     /locus_tag="VFA54_11695"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HZS51519.1"
                     /translation="MTSWRLELADIVFRSAVIYAFVLAGLRLGGKREAGQLTPFDLVL
                     LLLISNAVQNAMVGPSTSLAGGITAAATLLVMNLIAVKLVFRGGRVAAALRGSPTILI
                     HDGKVIPEHLKREGLTLDDLGQAIREHGVRDIADVYSAVLEVDGSISVLPNEDLKEPQ
                     RRRRRVRILRH"
     gene            complement(21006..>21861)
                     /locus_tag="VFA54_11700"
     CDS             complement(21006..>21861)
                     /locus_tag="VFA54_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZS51520.1"
                     /translation="VQAARWNYVLKPLGAPSLFRSTQAIYVGLFTNEILPLRSGEFAR
                     AYLVSHWTHLGYSVALSSVAIERLLDGIWLAIAFAVTASQVPLPPLIRDGAKVLGLVV
                     VASTTLLIAVLAEGLKVTHALERTRAGGRGIMSRILHFIDRLFEGLQAIGHSASLYCA
                     AGASVLLLLVQILTVWTMMRGFGLDYSFWVAAAVLFILHLGTAIPNAPANIGSYQVSC
                     VAALMLFGVDKATAAGFSLVMFAILTVPLLILGLLALLLSDISLQDIRRQLHHKLAFH
                     HLASHRER"
BASE COUNT         4176 a         6834 c         6586 g         4265 t
ORIGIN      
        1 caaagcaggt ctgcatagcg tcgggaggcg tcaggacgag cgcattcgtg cccgatcggt
       61 cccaagacgg cacaatgaaa gcgcaaggtc cggcagccgc tcttgccgcc gccgtcagct
      121 cttcgatgtc cgcaggcgcg atcaagggca catcgatcgg caccatgatg accgtgcgcg
      181 cgccataaca catggcgcgg cgggccgcca ggtcggccga cttcttatgc cccaacagct
      241 ccggctcgtc aaacaccatc gcgccggaac ggcgagcctg gtcggcggcg ccctggtcgg
      301 cggtcattac ggcgattcga tcgaggccgg aactggcgca gagaacactg aggacatgct
      361 cgtacatggc gcgcgccaga gtctcgcgga atcgctgttc cagcacgcgg ctcagccgct
      421 gtttcgcccg atcgggaggc ttcaccggaa gaagggcaaa aatcacagcg cctccagaac
      481 agttttcgcc agggcccttt tcgccgccgg atccttcatg atcgtgccgg tcacaacggc
      541 tcgcatcccc agtttttcaa tggcggccgc ctgggcgcaa tcgacccaat ccagcacgaa
      601 cacatccaca atgtcgcgat acagctccgc cacttgcagg gcggaggcgc gcagacccat
      661 gcttttcatc atacgggcgg cgggaccttt caaagcgcgc cccccgacga tcggcgaaac
      721 ggccgctacc ggcgcgtgcg tctggcgcag agtctcgcgg atgccgggaa cagccagaat
      781 cggccctatg ctgatgatcg gattcgaagg acacaggatt acagcccggg cctctttgat
      841 cgcctcaatt acgccgggcg ccggagcggc ccgcgcggcg ccctcgaact ggacgcccaa
      901 tacgcgatcc cgcgcgcggc ggcgcacaaa ataggtttga aattccagtt ctcccagggc
      961 tgttcgcact tttgtgcgga cgcgatcgtc cgacatcgga acgatagccg ctttgacacc
     1021 cagccttctt gcaatttgtg cggttacctg cgagagcgca gctcccttgc acagccgctc
     1081 tgaccggtag agatgcgtag ccagatcaga atccccgagc tgaaaccacg tctcgcctcc
     1141 cagccgcttg agcgcttcct ggcagcgcca ggtttccgcc gccaaacccc agccgcgctc
     1201 cggatcagca aggccggcga gcgtataggt caccgtatcc aggtcagggc acacgcgcag
     1261 gccatggatt tcggcgtcgt cgcccgtgtt gccaatgatg accaaccgag acggctggat
     1321 caggcgaacc aaaccatcca ggaagcgtgc agctccgacg ccccctgcca gggcgacgat
     1381 cttcttcatt cgagccccgt gatgcggacg cccgcgtgct tggctttatg gcggatgttg
     1441 agcgagatca gcagcgcggc aagactctcc agcagccggg cgttttccag agggcccgcg
     1501 tcgatggccc gtgcttcggg cagcaattcg atcaactcgc agacggcttt ctttgcctcg
     1561 gcgttgtcgc cgcacagcag cacgtcgctc tcaatgggcc gtccggctgc ctggagcagt
     1621 tcggcgctca agctatggaa ggcggccacc acggacacgc cttccggaat gtgctgcgag
     1681 gcctgttgcg ccgccgagcc cgcccacagc cccagcgtgc gcgacacgcg ccctccgatg
     1741 gcggcttcga gcggcacagt cgcatcgacc aggatcgccc cagagcggaa gctctggcgc
     1801 agcgacttca aagtagaaat ctgggccgcc agcgggaccg tgagaataac gatctccgcc
     1861 tccctcacag catccgggtt caagcgcccc tcggccccgg gaatgcgcgc cgcggccgcc
     1921 tgcgctcggc ttttatcccg ggaaccgatg aatactcgcg cccccgcagc agcaaagcgc
     1981 agagccagcc cgaaaccctc ggcgccggtg cctccgacga tagctatggt tcgcatgaag
     2041 gacccgccgg cctgattggg tgcggctctg tctcacacat gattcaagtg cggcgcggct
     2101 gcgccggaaa accccggcga aaaacgagcc aagccgaata gtggatcgac tttcgctcgt
     2161 tccagaacca agtctgccac cctctcgccc accgccgggc catgcttgaa agcgtggccg
     2221 gagccgccgc ctgcaatcca gacattcccg gcttgcggat gccggtcaat gatgaaatca
     2281 tggtccgggc tgttctcata ttggcacacc cggctttcaa tcagcggcgc atccttgagg
     2341 gctggaaagc ggaacgccag gtatttgcgt gcggccgcaa tgctttctgt ccggacaacg
     2401 cgatcgccgc cagtcggatc gaaacaatcg cctcttgtat cgtccgccag cttgaacccg
     2461 cgggcattcg ggatcccgta catcattggt tggccgtggt cgatccagac cggcaaccgc
     2521 ccctcgctga agcggtcatc gccggcaggc gcgccgaaaa agaacacttc ctggcgcgtc
     2581 gggacgatgc gatcgccgac ggcctgcgga aaaagttggc ccagccaggg cccgcaagcg
     2641 aagacgtatg catccgcaac caagctcgac ccatccgaga gaacgacctc ctgcatttct
     2701 ccgccccgga tagcgcccgg acgcgccagt agctgcctgt attcgccccc ttccaccaga
     2761 aagccctcga gaaccgccct gcaagcctcg cgcgccgaga gatagcccgc gtctctttcg
     2821 agaatcgccc attttacgcc ctcgaaattg atctgcgggt atcgcgctgc cgcctctttc
     2881 gccgtcagct cctccacgtc aacgccagtc tccctcagaa ccggcaaggc tgcccgttcg
     2941 taatggtcgt cttgcggcgc catccacaga accgaaatct gatggtacag ccggcggctc
     3001 catttctcct catgcgcccg ccacagatcc agagagcggc gcgccatctc gacgtagacg
     3061 cggtcagggc cgtaggtggc gcgaagaatg cgagtttctc cgcccgagct ggcccgcgaa
     3121 ttgccaggac cccaggcgtc aacgagcatc acccaaacgc cttggcgaag caggtgcagc
     3181 gccgtccagc cgccgaaagc gccggcgccg acaacgaccg ccgattttcg ccggcgcatc
     3241 ccaaggctgg ttcttcgctt caaagccatt cccttcaggc ttgtaccatg atacaggcgg
     3301 catggcaaag gcgccgttgg cggcccttac cctcggattt ttctttgccg gatcggagca
     3361 caaatacagg tgcgccccga gggcctggcg ttcatctata tcggggtatg gccggatccc
     3421 ttacaacacg catccggcgc ctgacgcgcg ccgaccatca ggccctgctt ggaatgtacc
     3481 ggcgatttga acccttaggc gtcgcggccg gcctgccgcc acgaaatgag caagcccggc
     3541 gcgagtggat cgagcgctcg ctggagcacg aaatcaactt cggagctttc tccccgaatg
     3601 gcgaaatggc ggggcactgc tttctggctg ccgatggacc agggtccgcc gaaatggccg
     3661 ttttcgttca tcaggatttt cgcaaccagg gcatgggcag gcagcttata aacgcggcca
     3721 ttcaagaagc gtgccggaaa cacatccaga gaatctggac catgacttcg gccgacaatc
     3781 tggcggcgtt ggctgtgctg aagcgctgcg ggttccaggc gcgccgaggt cccgtaacag
     3841 ccttcgacct ggaactcgaa ctcgagccct cctgcgccgg gaccgccccg gaatggagga
     3901 actgacgacg ccggcgcaac cggacgggac tctctataaa ttgccttctt caaaaaaaat
     3961 caccgggcgg ccctgtcgaa gcagccgccc ggcgcgcgca ctatgctttt ttgaaaggag
     4021 actcagttta tatacacgaa ctcgttcagg ttcaggatga cgtcgcacaa gtcggtcagc
     4081 gctgcggttt ccgggtccga tttgactgct gcgctctcgt ccgtctgctg cgccttcttc
     4141 gactggtggt aggcgagctg tttttccagg aacttcaaat tgccttgtag ctcgctcggc
     4201 gtcggatccc ggctgagagc gatgttatac gctctcttca cctgagccgc tttgtctgcc
     4261 cccgcctctt gctccacccg ctgcgcgaaa tacttcgctt gcagcagcac gaactcgttg
     4321 ttcatgagtg tgagggcctg cgtcggcacc gtagtcacgg ttctcgcctc gcaagtgaca
     4381 ttcacatcgg gctcatccag aacgtcgaac atcggatact tcatgccgcg tttccagtaa
     4441 gagtaaatgc tgcgccgcca ggtagagggc tcttccttgg tcagcaccca tttcccttga
     4501 cggaatccct cccgcacgct cttgggaatc gaggggaaga acggcggccc gccggcttgc
     4561 aggttgagct gcccgctcgc cgacaggatg atatctcgga tgatgtctcc ttccagacgg
     4621 tttagcggga acctccacag atacgtatca gtaggatctt tacggagatc ttcggcgtca
     4681 taaaaggccg acgccatctg atacgcctgc gacgtcatga tcagccggtg cattttcttg
     4741 atgctccagc cttgccgcac gaactcggtg gccagccagt cgagcaattg cgggttcgac
     4801 ggagggctgc cgagcttgcc gtaattgctc ggagtgctca cgattccctt gccgaagtga
     4861 tgctgccaga tgcggttgac catgacgcgg gcggtgagcg gattcttctc cgaagcgatc
     4921 cattcagcca gggcgcggcg gcggccgctg gtcacccggc cgtcggccgg cttgatcgag
     4981 acggggggtg gattatccgg atcgatcagc acttttataa agcccggctc gaccaccggc
     5041 gccttctcgt cgtcggcgac cacttggcca ttggcgccga aatggacctt cggcggctcg
     5101 tagttgtcgc ctggatcggg aatgtatttc cccgtacccc agttgggccg gttgcctttg
     5161 ccaggcagcg gttcgtcgcc agggccgtcc ggcgtgaatc gatagtcgcc gtcccgaatg
     5221 ccttcagcaa tcggaatcgg aggcggcatc tgcttttcga gcgcagcgat tttatccagc
     5281 agaacctgcc gtttcgccgt atcttcatcg ttcagcttca tcaggcggcg ccggtaggct
     5341 gctccgttga aggcggctgc cgcggctgca tcgggatctt ccaatccgtg caggaactga
     5401 gcggcgatca gcttttgccc cggggtccgc ttgtcctcag gcgtgtgcac cgccacttgg
     5461 atgtcctcag ggtagctctt cagcatctcc tcgaaccgtt tttccttgta cggcttctcg
     5521 atgcgcgcga tttcaatccg cagaggagta atctgatcct ggatttcctt caccttcttt
     5581 tcatacgcag ccgcctggtc aggaggcgcc agcgggaagt cgtagtttac gaaggggaag
     5641 aacatcgcca tcatgcggta ataatccatg cgcccgatag gatcgaactt gtggtcatgg
     5701 caccgggcgc agtttaccgt caagcccagg aacccccgcg acgtggtccc gatcatgtcg
     5761 tcgagatact cgtagcgata gtatggattg tccttctcgc ggaagcgcac ccgcggcccg
     5821 acacggttga aggtcgtggc gatcaggctg tcgtaggtgg gatgatcgac ttcatccccg
     5881 gcaagctgct cgatgatgaa ctgattgtac ggcttgtcct cattgaaggc cttaatcacg
     5941 taatcccgat agcgccaggc gttttcgaag tctcgatcgt gctcatagcc ggaactatcg
     6001 gcgtagcgcg ccacatccaa ccagtgccgc gcccatcgct ccccatagtg ctctgaggcg
     6061 agcaagcggt cgatcagctt cccccacgca tcgggcgatt tgtcgttgac aaaagcgtcg
     6121 acctcctccg gcggcggcag caggcccgtc aggtccaggt acgcccgccg gatcagcgtg
     6181 cggcggtccg ccatcggagc cggcgtcaga cccttcgcgt ccatctgctt cttgagaaaa
     6241 gcgtcgatcg ggttggcgct ccagcgcgga tcgttcactt cgggaaccgg gtagcgcacc
     6301 ggccgctgga aagcccacca ctcccggtcc ttggcagtga tcacgcggtc gagattggcg
     6361 ggctttttcg gcaggctgtt gtacagcgcc accatgtcca cgctcttgtc ccactgcgcc
     6421 ccgctgtcaa tccagtcttt gatgatcttg atttccttgt ccgtcagcgg ctcgccgttg
     6481 tagggcatgc ggggatcttc ctgcgcggta atccgccggt agacgtgact gtgctcggca
     6541 tcgccgggga tgatcaccgg cccgtgcatg ccgcccttct tggccccttc cggggttcgc
     6601 aggtccaggt tcgccgatag agtcgccgag ccgtggcact gcatgcattg agccagaatg
     6661 ggcgcgacgt cacgggaaaa cgagatgctc ggcgtctggc ccgctgcgtt tagtgaaaag
     6721 agaaaaaaag gaataagaag cgcgaataag caaccgcgca cgtctgtgca gagcacgcgc
     6781 ctcgtatgca tatgccacct cccaaaatgc gggcgcttta cgagaatgcg ctctgtaaat
     6841 tttgttaccg ccgcgatgcc gcgaagtcaa taggatatta ttgatatcta gttatcaaag
     6901 tcaatattct caggcgtctg ttgactaagt cttggccgga tgtaaagaag ttgttgagaa
     6961 tgcaaagaga ttgttgagac ccgccgccgg cctgctcaga accgtgtgtt atagtgaggc
     7021 gtttcgagag cgcgtacacc ccttcggttg gggtttgagt catggatgca ggccaaatcc
     7081 ggcgtatcgc ggtcatcggc gcgggtcaaa tgggacgcca gatcgccatg ctggcggcgc
     7141 tcggcggcta tgcggcggcg ctgcacgaca tctcggaagt ccagatggaa agagcgtttg
     7201 ccgaaaatcg ccggcggctg gaaaagcagg tcttgcgcgg caagctggcc gcggaggaaa
     7261 tggaagccgc gctcggccgg ctgcgtcccg cgatgacctt ggaggaagcc gccggggcgg
     7321 ccgatttcgt catcgaggcg gtagtggagc gcctcgagga gaagcggaaa gtcttcgcca
     7381 atctcgattc gatttgtccg gctcacgcaa ttctcgccac taacagctcg tatatcggca
     7441 actcccggct ggcggccgtc acccgccgtc ctgaaaaagt gatcaacatg cacttctttt
     7501 ttccgccctt ggtgatgcgg ctggtggaag tcgtcaaggg cgagaagacg agcgaagaaa
     7561 cagtcggggt caccgccgat ctcgcccgcc gcatgggccg cgagccggtg gtgctccgca
     7621 aggagcttcc gggctttttg gtaaaccgga ttttgcgggc gattcagtcg gaagcctact
     7681 acctgctcga aaacggagtc gccggcttcg ccgacatcga caaagcagtg gagctggggt
     7741 tgaactttcc gatgggccct ttcaagcttg ccgacttcag cggcctcgac gtgggctatt
     7801 acagccgcat ggatcggtac cgggaaaccg gcaatccggc cgagcagccg cctgctgctc
     7861 tgagaattcg cgtggagcgc ggggatctgg gccggaagac cggccggggc ttttacgatt
     7921 actctgtcga tcccccggcg cctaccgaag attgagccct ccatagccat gcatccggca
     7981 gcggcacaag gactgcgcct ctggccgaaa tctctttatc cggaaacgta ccaaaatgtt
     8041 ttaatgtgcc gaacctcgca gcgggcgcct tgcaggcaga ccagcgccgg aaacgcgtcg
     8101 gaatggatca tctccggcta ttttttcgcc gccccgatgt atggaacagg ccggatttaa
     8161 tttccgtatt tggtctgtaa gatgttggtc tttaataaga taggtctcca catgccactg
     8221 ttgccatgtt tggcagacgg cgtgctgccg ctcggggtgc atggcgctcg cactaacggt
     8281 aatgatcccg ggtattttgg cgttgctggt tttcatcacg ctgatcctct ccctggtcgg
     8341 gccgctgcac cgggacggcc gcatgcgtta actctccact ccctgcgacc gggttttctc
     8401 gggcgtctct catcagacgt ttggtactcc attactacga ccacggtggt aggtgtatta
     8461 gccgagagac aaccgcagaa gtcgcgtgtt agagtactgc gatagggaga agacggtgcc
     8521 cgcaagtctc gcaagagttc ttctggtgga cgatgatgct cagttcttcg aagaagtgaa
     8581 gagccgcctg cgcaccgagc gggatatcat cataggctgg agccgcacgc gcgccctcga
     8641 agacgtcccc gatgacgaat atgcgcacgc cgacgtcgtg atgaccgatt ccgacaagca
     8701 cggcccggag cttgctgaca ccgtgtcgcg ggttaaatcc cgtttccacc acctgcaatt
     8761 tctcatcctg ggatcggccg aagacgacac ggtcaccatg atcttgctcg aatcgggagc
     8821 gcgcggttac ctggatttgc agacctcggg cgagaacctg gcttcggcga tccggcggct
     8881 ggcgtcggga ggcacctggg tgcccccgca catcctggcg cgatacatgg acctgatcca
     8941 aatggttgag tttctgccca agaccggcgc ggaaaacgat ccctgggcgc aattcactgc
     9001 gcgcgaacag cgcgtactca ccttgatggc ctgcgggcgc agcaataaag agattgggag
     9061 agtcattggc gtggacgaaa ctatcgtctc ctccacgata caccagctca tgcaccgcac
     9121 gggcgcggtg aaccgcccca gcctcattgc ctactactta taccataagc tcttccgcgg
     9181 cgaaagcctg cccgccatgg acgcggaaaa agtcctcgaa aattagagct tcccgttaaa
     9241 tagcggccag cacgccgcgc aagtacccga acgactccgc catgccgcga acgggatgtt
     9301 ccgcctccgc ttcgtattcg agcgcggcga gctgggtaaa gtggatttcc ctcagcacgc
     9361 gcagcatggc cacgacgtcc agcacgcccg tacccaccgg gacgttgtgc ggctcaacat
     9421 cctggggccc ttggggagcg gccaggtcct tcaagtgcac tccgtacagc cggggagcgc
     9481 acttgcgcag cgcatccacc ggatcgacgc ccagcaagaa cgtgtggccg acatcgacgc
     9541 agcagccgat gcggctgtca agcccgcgga cggcgtcaaa cacacccagg ggagacggcc
     9601 acactttgtc gcgcgggccg tggttgtgaa tcgccacctg gatgtccatt tctttgacgg
     9661 ctttctcgac tgctggtaga gcggcccgtt cgggtgaaat cgtcgccacc ggaacgccca
     9721 gatccttggc gtactgcaaa gcatgccgga tctgcgcttc gtcgtttttc aggtagatga
     9781 ccccgacgcc cataatcttc acaccggcgt cttccgcttt tttcttcgcc gccaggcgtt
     9841 cttcgcgcgt cgaggaaagc ggcagatgca cgtctttcag cgagatgtat tcgatgcgga
     9901 tgcccttcaa ctcctccagc gttctctcat acgtcagctt gcgcgtcgtg tacgaggtga
     9961 ggccgatctt caggccatga aacgccggag cgggcggctt catagcccag ttttggcccg
    10021 cggcggacat ggctccgccg gcgcctagaa cagcgagttt gagcgcctcc cggcgtctca
    10081 ttttctgatc cccgacgttg gtgtgcatct aattgcctcc atcaagcccg ggttacggtg
    10141 ggcagatccc gggtttcatg cagtttcatc gagcggcggg cttcggccac cacggccaaa
    10201 aaccggcggg cgttctcttc cacgacttcc agattgccca tcgccagcgc ctgggcgttg
    10261 accatctccg atccgacggc caccgccgag gcgccagccc gcagaaagtc agccgtattt
    10321 tccaaattga cgccgccggt gggaaccatc tccacctgag gcagcggcgc cttgagcgcc
    10381 cgaatgtact tcggtccccc gacgttgccg caggggaata ccttcaccag gtcagccccc
    10441 gcatcccaag ctgtaagcac ttccgtagga gtcagggcgc ccggaataac cactttcgaa
    10501 taccgatggc agatttcaat cgtaggaagc cgcagcgttg gagtgacgaa aaactcagct
    10561 ccggccagca tgcaggctcg cgcgctctcg gcgtcgagca cagtgcccgc acccagcgtg
    10621 agccggtcgc cgaactggtc ggccacctgt tccagaactc gcagagcgcc cggcacggtc
    10681 atggtgattt ccaccgcccg gatgccgccc cggtaaatcg ctcccacagc ctggatcgca
    10741 gcctcaggac tggaggcgcg cacgatcggc accacaccga tgtccagaat gaaggataat
    10801 gtcttagact ttgccatatc atcttgagta tcgccgataa gctatcaggc gcggaggttc
    10861 ggatgcaacc taatttgctc gccaaatcgc cgttgcagga gccgggagta tatcccattg
    10921 tcaagcgcgg aagcggcctc aaacatcttt ctttcaccgt tgtccaaatg ggtccggctt
    10981 tgcgggaata ttccgccgaa agcggagagg aggaattggc cctggacttt tatcccggcc
    11041 aggtccgtgt cgaagcgcaa tctccgcagg gaaatatcga acgcaccacc cgcttgcggc
    11101 ggtcgctgaa ggaagtggga gaaatgttgt acctcccgtc cggatcgcgc ttccggatca
    11161 ctctgctcag cgacaacgcc cgggtgggaa tcgccggatc cctgggcaag ccgggagtcc
    11221 cgccggtgtt tatttcccag gatcaggtga tgagcaagaa agtgggaaaa ccgagctggc
    11281 tgcgcacggt tcatacgcac atcggcgaca acgtcaacgc cgcgcacctg atcgtggggg
    11341 aaacggtgaa cgacccggga aactggtcct caagcccgcc gcacaagcac gaccgccacg
    11401 cgccgccagc ggaagtacct atggaagagg tatacttttt tcaggtggag ccgcgcgggg
    11461 ggttcgggtt tatgcgcgtc tacaccgacc cggcggaccc agaacctttc gattgcgttt
    11521 ataccgtcga ggacggggat accgttctga ttcccaaagg ctatcacccc gtagcggcgt
    11581 ttccgggtta tactctgaac tacacgtggg cgctggccgg cgaaggccgt gtttacggcg
    11641 cctggacgga cgacccccgg cacacctgga ttaaggataa ttagcccata ccggcggcag
    11701 gatcctgcat ggagagaaag tacaggcagc ggggatacca ggacgacgac agcgagcggc
    11761 gggcggaaaa gcccagtcgt cccccgggcc cgcgggaaac gatcgggccg cgcctgcctc
    11821 aattcccggc ccgcgtcacc gtagcccgct gcgccaactg cggaacactg cttccgaaag
    11881 attttgatcc caaggggcaa tgcccgcgct gcggattcga actgcattgc tgcaagcagt
    11941 gcgtccactt cgatacctcg gcgcggtggg agtgtacgca gcccattccg gcacgcattg
    12001 cgcgcaagga tgcccggaat gactgcgcat tttacgagat tcggatgtcg agcgagcggg
    12061 agacgacatc cggccgcggc ccctccaggc ccgatgatcc ccgccaggcg ttcgaaaact
    12121 tgtttaagaa gtgacgacgt gtctctggtg tgctggcagt gcccgccgcg tccctcttaa
    12181 caccagctgc ccctgggcaa gttcagactg tttcgccgcg cggctgtcct cctccgcata
    12241 ttcccaggcg gctgttgtgc caaccttcgc ttctccccgg ccgtgatgca cgccgtgccg
    12301 gaaaccagcg atgggttctg cacgtcacgt aatatggttc acccctcatc gccattttgt
    12361 ctaaggagca gcgggaatga aagccgccca tttgcgagcg ctccttttcg ccggtgcagc
    12421 gttggcagcg ggcgcctccg gctgcgcgca taaagcgccc gtggagccgc cgaaaccgcc
    12481 gcttgtgccc gctgtgtttc agccgctcca ggatgctttg cagaagtcct atctcgagct
    12541 attccaactc gctcccacgc tcgagttcag caacacccag atccagaaca tgaggactta
    12601 cctcgagcag gcgaggagat attgcgtcgg ccagtataaa aagaagagcg accaatacag
    12661 cgccgacctc aagcgattgg agggcaacct caagcaggta agcgccacgg ccagcgacac
    12721 gcagaggcac gatttgcact gccagatcca gaacacgcgg gccctgcaaa gccagaccga
    12781 ggtgcttgcc gatcacgcga tccccgttgc ttatgaaaat aaggaagcca agctggatct
    12841 gattcagcag tggcccgggg atctgaaaca gatcaagcag caaatcgcgg atggctctta
    12901 tctgaagcgc cagtgggggg acgtcaaaga catcggattc cgggaaatcg cctccggcca
    12961 gaaggacgac atcaagaccg gcgaggatgc gatcaaacag atgaaacagg ccggtttgat
    13021 gccgaaagag ctggataaca aggcaatcac ggagtatgtg gaggacgtgg cgcagaaagt
    13081 ggcgaagcat tccgatttgc agatcccgct ccacgtaacc attctcaatt ccaaggagat
    13141 taatgcgttc gctctccccg gcggcttttt gtttgtggaa cgcgggcttt tggacgccgc
    13201 cgacgacgaa tcggaactgg cgggagtgat cgggcacgag atcggccacg tggtcgcccg
    13261 gcacggccac aagctgatgc aaaaggcgac gatcgagtcc gtcgtatttc aggcggccca
    13321 ggtggccgct gtgttgctta caggcggcgc cgccggaatc ggcacttact atgctttgca
    13381 gtatgggttc tacggcctgg gtctcgccct caacctgaat ctcctgggcg tcagccggga
    13441 gtacgagctc caggctgacc agcttggaat tcagtacgct tggaacagcg gctacgaccc
    13501 ttcgggattc attcgcttct tcgacaagat ggcgacccaa gagggttatg taaacggggc
    13561 aagctggttt cgggatcacc cgccttttta ccagcgcatg ctcgacgccg agcgtgaaat
    13621 catgtttctc cccaaaaaag aaaatttggt tgtacaaagc tccgccttcg agcagatgaa
    13681 aaaagagctc aagccggtta ccgctaaagc ggaagcggag gagaaggggc ggcccagcct
    13741 gttggcgccg gagcaaggct gtccggctcc ccagaaaatc gagtacaagc ccggggaacc
    13801 gattgaaacc atatgttcgc tgccagggac agtgaccaca aaggtggcaa gcaaataacg
    13861 gcgctggcgg gcctggcgtt tggagcaatc cttgtcgctt ttctggcccg gccggtcacc
    13921 gctcagtttc cgaggcttgt ttccctctac gctaccgtcg agcgcgatgg aggcctggtc
    13981 cgcggcctcg gcccgcagaa ctttcacctt tacgaggacg gggacagccg ccaattccat
    14041 ctagagccgc ctgagactcc tatggtcatt gcgctgctgg tcgaatatag ccagcgctct
    14101 tatccgtatt tcaacgatat ccaaactgcg gtcgagagtt tcgtcaggca ggcccccgag
    14161 ggaaattggt acgcgttggc cactttctcc cacaaccttg aggtgcaggt cgatttcacc
    14221 aagcaagtca tgcaggtctc gaatgccttt tcacagctcg gcgagcccat gtggaacgag
    14281 gtggatacct acgatgccgt gtatgagatg ctcgacaaga tggaaagact gccgggccgc
    14341 cgggtgctga ttgtgatcgc ctccggactg gatagtttca gttcccatac gctcgatgat
    14401 gtgcagaaga aaatcgatgc cgtcaacgtc gtgatttacg gagtgggagc cggatccatg
    14461 ctgcggggaa tctacgagcc gtacctgggt acgatgcagg agatgagcct attgcaggcg
    14521 caaaccttcc tgcaaatgct ggcggataag agcggcggcg aggcgtggtt tccaaagtta
    14581 gaaggcgctt ttccggatgt catagaaggc attttccagg atctggagaa tcagtacagg
    14641 ctggtctatg aatcccacgt gcctgccgac cacaagctcc acaagatcaa actcgaagcc
    14701 ttccagatca taaatgacaa gcggcaaaac tttaaggttc gcgtccgcga aggttggcga
    14761 ttctagacca gcttgtgcga atgcggcgaa gactgcgcct tcggcccccg ccggggtcgt
    14821 tttgccattc cgtcgagcgg acgacgtctt cgagccgagc acccctgacc agcagattcc
    14881 tcgctccgaa tccgatcgct cgctgggcgc cgtgagaagc gcggcttgtt cgccgggtgt
    14941 ccgaaccacc gttaacgcgc cgccggccga aggctgatat catgaaattt cttaagttgc
    15001 tgcgcctccg tggctgatcg ccatccgatt cttcgcctgg aacaggtgac caaggattac
    15061 ttatcgaacg gtgcggctgt ccgcgcgctt gatgccgtct ccctcgaggt ccgccgaggc
    15121 gagtttgtgg ctttggtcgg gcgcagcggg tgcggcaaga ccactctttt gaacatggcc
    15181 ggtgcgatgg acttccctac gtccggccgg gtctggctcg acggcgcctg tacgagtgaa
    15241 ctgaatgacg ccggactgac ggcgttaaga cgcagcaaaa ttggtttcgt atttcagttt
    15301 ttccagctcc tcccgaccct ttccgcggtc gagaatgtgg agttgccgct cctgctcgcc
    15361 gggcgttcgg gcgcgcgtct ccaagctctc gagcggctgc aatgggtgga gatgcaaact
    15421 ttggcgcacc gccttcccca ccaactttcc ggcggccaga tgcagcgggt agcgattgcc
    15481 cgcgccctag tccacaaccc gcagatcctg ctggccgatg agccgatagg caacctggat
    15541 accgctagtg gcgaggtagt gctgaaactc ctgcgccgcg ccgcttctga actgaaatcg
    15601 tccgtcatca tggctacgca cagcgcggaa gccgcagccg cggctgatac tgtggtccgc
    15661 ctgcgcgacg gccgcattgc agaaatccgg cgtgtatgac ctcccgatgg cggctctttc
    15721 accggttgat cctgcggccg cttgtccacg agcgattgcg cacggcgctc actgtattcg
    15781 cagtagcgct cggagtggcg gtagtggtgg ccatcgatct ggccggagcg gcggcggcgg
    15841 gctcgttccg gtcctcgctg gagtcgctcg ccgggcgtac tgatctcgat ctcagcgcga
    15901 tcagcggcat cgacgagcgg gttttcgtgc gcctctccga gcttcccttt cccgctcagt
    15961 tccggcctta cattgacgac ttcgcccgcg tcgagagcac gggggtgacc gtgccgctgg
    16021 cgggcttgga tttgttgggc gaaagcgact ggtcgcttgg cggagactgg ggaagcggtt
    16081 ggaacgaccc gcctgactta tccagctccg tctgggttgg cccgcggatc ggcgccgcgg
    16141 ccggttcgaa aatccggctc actctcaacg accgcagccg tgagtatacc gtccgcggtg
    16201 tgctcaaact ccaagggctg gcccgctata cccaggatga tctgatcgtg atggatattt
    16261 cggccgccca ggaggcgctc ggcaaagtgg gacgcctgga ccgcatcgcg gtaaccctgc
    16321 ctagaggcgc cgatcttgcg gaatgggaac agcgcttgcg cggcgtggtc cccgccgacg
    16381 tttcgattga gcggcctgga gcgcggagcg aagaaaacca gaaaatgctg agcgcttttc
    16441 gctggaatct gcgggtgctc agctacattt ccctcgtggt cggcgcgttt ttgatttaca
    16501 acaccgtcgg cgtttcggtc gtgcggcgcc gcacggaaat cggcgtttgg cgggcgctcg
    16561 gggccacccg cggcgtggtg ctcggcgcat tcctggcgga ggcggccttt ttcggtctca
    16621 ccggatctct gaccggcctc ctgctgggca ggcttatggc cgaaggcgcg gtgcgcctgc
    16681 tcgcatccac cgtgcagtcc ttgtacgtaa gcagccgccc ggcgcccatc gagttcactc
    16741 cggctaccat gttgctgggc gccgcaacag gcctcggagt cgccctgctt tcggcgctcg
    16801 ccccggcccg cgaggcgaca aacatttcgc ccatcgaggc aatggcccgc ggccttcgag
    16861 aacaccaggc gcgcgtttcc tggcggcgtg atttgctggc cggggcagcc gttactctcg
    16921 gcgctgcggc ggcttcgcgc ctgcccccca tccaaggcaa gcccgtattc ggctatgtcg
    16981 cgacatttct cctgattgcc gcttcggctc tggcgacgcc ggccctggtg ctgggggtat
    17041 cgcggcttgt ccgcatgtcg ctcggcgtcg aaccggctct ggctgcccgc agcctggcgg
    17101 cgtcgctcaa ccgcacctcg gtcatggtcg gcgctctggc tacggcagtg gccatgatgg
    17161 ccagcgtagg catcatggtg ggcagcttcc ggcagactgt gattctgtgg atgaacacgc
    17221 agcttcgcgc ggatctttac gtcaggcccg ccgggcgcaa tggggccggt caacacccga
    17281 ctttagattc ccggctcgca gaccgcattg agcaattgcc gggcgtggac gccgtggacc
    17341 gatttcgcgc ttacgaaatc agcttccggg gcctgccatg cacgctcgcg ggaggcgaga
    17401 gcgccatcgt aaggcgtttc ggccgcctgc gctttctgag cggagaggat cgaaatcgca
    17461 ttctcgacga actgccccgc ggcgattatg cactggctag cgagccgctg gcgaacaagc
    17521 acggattgcg cccgggcgat acaatcacgc tgccgctggc gggcgcccca cgccgctttc
    17581 aaattctagg tgtgtattat gactattcga gcgagcgcgg attgatcatt cttgaccgct
    17641 cggttctgct caagtatctg cccgacccgg cgccctcgaa tctggctgtt tacgttcggc
    17701 gcggcgttga tcccgagcag gtccggcgcg caatcgaggc cgcggccgcc ggccacactg
    17761 tcttgatcga gccgaacagc agactccgcg cggaggcgat tcgaacattc gatcgcacgt
    17821 tcgccatcac ctacgcgctc gaagccgtgg ccattcttgt cgccgtcatg ggaattgcgg
    17881 gcgcattgct ggctctggtg attgaccggc ggcgggagct gggcttgctt cgattcctcg
    17941 gcgcctccgc cgttcagatc cgccgtttgc ttttcttcga ggcggccctg ctcgggttgc
    18001 tggccaacgc tctgggcctg gcactgggag gagtgctttc gctgatcctg atattcgtca
    18061 tcaataagca atcctttggc tggactatcc agttccactg gcccgccgcg ctccttgccg
    18121 ccgggctgac cctggtttat gcagctacgg tgctggccgc atggctgccg gcgcgggcgg
    18181 cggcgcggct gaaacccatc gaggtcgtgc acgaggaatg aagcatttct tgtggatcgc
    18241 cgctctcgtg ttccagatag cccgccccgg ttatcgctac gagtttccgc gcgaccattt
    18301 caatcacccc ggctttcaaa ccgagtggtg gtattatacc ggcaatttgc aaacccccga
    18361 gggccgcccc ttcggtttcg agctcacctt ctttcggcgc gctgttgccg ggcgcgaaaa
    18421 tcgtccggcg cacgccaggc cggattccac gtgggaactc gacgacgttt atctggcgca
    18481 tctcgcgctg agcgatatca ggggccggcg cttttatgcc gcggagcgtc tgaaccgggc
    18541 cgggccgggg cttgccggcg cttccctgga gcagaggcgc atctggaacg gcaactggca
    18601 agcccgctgg aacggcgccc ggcagagctt gcaaggcgtg gctgaggatt tctcgttcga
    18661 gctcgagctc gatccgcaga aaccgcccgt tattcacggg cgcgacggca tcagccagaa
    18721 agccaacgga gagggccgcg cctcgcacta catctctttc acaaggctgg cagccgccgg
    18781 cgcaatcact cttcgcggcg tgcggtttcc ggtgtcaggc gccgcctgga tggaccacga
    18841 gttcttcacc catcaactgg atgcgtcgca agccggatgg gattggttca gcattcaagt
    18901 cgacaacggc acggaactga tgctgttccg cctgcgccgg aaggatggca caatcgatcc
    18961 ctactccgcc ggcacgtttg tcgaccggca agggcgcgcc caacacctcg aatttcatga
    19021 cttcgcgctc gaaccgctcg aaacatggac aagtccggcg actggagcgc gctaccccgt
    19081 gcactggaaa atccaagtgc ccccgctcgg aatcgacctc gactgctcaa cggcgctccc
    19141 gtttcaagag ctggtcgatc gccgccagcc cgagctgaac tattgggaag gcgcggttac
    19201 ttaccatgga cgcagcgggg acaaagcggc cagtggggtt ggttatctgg agatgacggg
    19261 ttatggccac ccgctccgga tggattagcg cccgcaattc acccggacct cagttcgccc
    19321 tctgttacaa tgccagggaa ttggcaatgc cgatagcccg gctccgacgg cgccgccagt
    19381 tcctgcaatg tgcggcgggc gccgtgcttt tcgcatgccg cgacgggacg attgccgcac
    19441 cgggaagcat gaagaccgct ctgctcgccg acgccatata taaagagcac gtcacaggcc
    19501 ctgggcaccc cgagcggccg gagcgttgcg atgcggtgac cgaggctctc cacaaaggcg
    19561 gcctcatgcc atctcttgac cggatcggcg ttcgtccagc cactgaagag gaaatcggcg
    19621 cttgccacac ccgcgcatac atcgaattag tgaaacgcga gattatggcc ggagccagcg
    19681 agctcagcac gggagacacc aacgtcgggc cgcgctccct ggaggttgcg ttgcgcgcgg
    19741 ccggcggagt gctgaatgct gttgacgccg taatctcacg gaaagcgaga aatgcgttct
    19801 gcgccgtccg cccgccgggc catcatgcga ggccggcgca gggcatgggg ttctgcatct
    19861 tcaacaacgt cgcgatcgcg gcacgctacg cccagcgcaa acatcacgtg gaaaaagtcc
    19921 tgattgcgga ttgggatgtg caccatggaa acgggactca ggatatcttt tacgccgatg
    19981 gatccgtttt cttcttcagc actcaccagt cgccctggta tcccttcacc ggcagggccg
    20041 acgaaaccgg tgaaggcaac ggcgccggtt gtaccatgaa ctgccctttt ccggcgggct
    20101 cgggccgtga agagattgta ggcgcgtttc gcaggaagct ggccccggcg gcgcaaaatt
    20161 tccggcccga tctggtgctg atctccgccg gtttcgactc ccgcgccgac gatccgctag
    20221 ggcgttttac actctccgac gaagatttcg cggaactgac ggcgatcatg ctcgaaatcg
    20281 ccgataagca cgcaggcggc aggctggttt cggtcctcga aggcggttat aatcttccgg
    20341 gccttgcctc ggccgctctt tcccacgtga aagctctgac ttccgcgtag gcacttttcc
    20401 ctcttccgcg ctgttccatt tcgatgatgc gggccatgaa tgtctcggcc gttttttgcg
    20461 ggagcgcgcc tggtccgcct aatgccgcag aatgcgcacc cggcgccggc gcctctgcgg
    20521 ttccttcaga tcctcattgg gcagcacgct gatcgatccg tccacctcca ggaccgcgga
    20581 gtagacgtcg gcgatatccc gcaccccgtg ctcccggatc gcctgcccca ggtcatccaa
    20641 ggtcaagcct tcgcgcttca agtgttcagg gatcacttta ccgtcatgga tcaggatagt
    20701 cggcgatccg cgcagagcag ccgccacgcg gccgccgcga aagacgagct tcacggcgat
    20761 aaggttcatc accagcaagg tcgccgccgc ggtgatgccg ccggcaagcg aggtgctggg
    20821 accgaccatg gcgttttgca cggcattgct gatcagcaac agcagcacca ggtcgaacgg
    20881 cgtcaactgc ccggcttctc ttttgccgcc gagccgcagg cctgcgagca cgaaagcgta
    20941 gatgactgcc gagcggaaaa caatatcagc gagctccaag cgccagcttg tcattttgcc
    21001 tccgactatc gctcgcgatg cgaggccaga tgatggaatg cgagcttatg atgcaactgc
    21061 cggcgaatgt cctgcaagga gatatcgctc aacagcagcg ccaagaggcc gagaatcagc
    21121 agcggcacag tcaggatcgc aaacatcacc agcgagaaac ccgccgccgt cgctttgtcc
    21181 acaccgaaga gcatgagggc ggccacgcag gaaacctggt aggagccgat attggccggc
    21241 gcattcggta tcgcagttcc caggtgcagg atgaagagca ccgccgcggc gacccaaaac
    21301 gagtagtcca gcccgaagcc cctcatcatc gtccataccg tcaatatctg gaccagcaga
    21361 agcagcacgc tcgcccctgc cgcgcagtaa agcgaggcgg aatgcccgat cgcttgcaag
    21421 ccctcaaaga gccggtcgat gaagtgcaaa attctggaca ttatgccgcg cccgccggcg
    21481 cgggttcttt cgagcgcatg ggtcactttc aagccctcgg ccaggaccgc aatgaggagg
    21541 gtggtggaag ccactactac cagaccgagc accttcgccc catcccgaat cagaggcggc
    21601 aagggcacct gggaggcggt tacggcaaag gcaatcgcca gccatatgcc gtcgagcagt
    21661 ctctcaatgg ctaccgaact gagcgcgaca gagtagccga ggtgtgtcca atgcgagact
    21721 aggtaggcgc gcgcgaattc gccgctgcga aggggcaaga tctcgttggt gaagaggccg
    21781 acgtagatgg cctgcgtgga tcgaaacagc gaaggggcgc ccagcggctt tagcacatag
    21841 ttccagcgcg cagcctgaac t
//
LOCUS       DASREK010000223        12091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1267690, whole genome shotgun sequence.
ACCESSION   DASREK010000223 DASREK010000000
VERSION     DASREK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267690"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFA54_11705"
     CDS             complement(<1..293)
                     /locus_tag="VFA54_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51521.1"
                     /translation="MAESEGGRPITADAVPVIEPGYTYASVTDKISAIVLSRRPPLAW
                     WLGFGIAFLLVLLLFGAVAYLLATGVGIWGINIPVAWGFAIVNFVWWIGIGHAG"
     gene            complement(280..3288)
                     /locus_tag="VFA54_11710"
     CDS             complement(280..3288)
                     /locus_tag="VFA54_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HZS51522.1"
                     /translation="MKDERHTDAFSLARGRLASGPRHWRSLEELTGSEEFLQFLRSEF
                     PEEAPSWADPVSRRELLKWMGASLALAGLTACSPQGAGRPDWPPPPMRKVVPYVRAPE
                     EVTPGKPLFFATSMVLGGFAKGLLVKSHTGRPIKAEGNPEHPASLGATDAFGQASVLT
                     LYDPNRSQVVTREGRISTWPAFAAALNGLREEQRSKRGAGLRLLTETITSPSLAGLIR
                     SVLAEFPAAKWHQYEPVSRDNARAGALLAFGEDVNPVYRVERADVILSLDADFLWEGP
                     GSIRYTHDFAARRRVSGGRASLNRLYAIEPSPSITGSMADHRLRARASEVEDLARELG
                     RRLGIAGAAGEARANTAFVDALARDLERNRGSSLVIAGAQQPPAVHALAHAMNRILGN
                     EGETVVYTDPVEANPVDQTESLRELVEDMQAGRVDALIILGGNPAFYAPRDLGFAAAL
                     SRVRFKAQLSLYDDETTVLCNWHIPEAHFLESWGDARAYDGTVSMIQPLIAPLYQGKS
                     AYEVLAALLGQPAAATHDLVHDYWKVQRRWESFDDDWQKALHDGLIAGTALPPKRVNL
                     KAGFPAQIGAPPRAAGLEIVFRPDPTIWDGRFSENGWLQELPKPMSKLTWQNAAMMSP
                     ATAARLGLDSSDVADLRFRGATVRAPVWVMPGHADDSITVHLGYGRRSGRPGSDAGFN
                     AYALRHSDQPWFGYGVEIARTGLRSDLVSTQNHFSMEGRHLIRSATAAEYEKRPQFAQ
                     EVEERPKPDFSLYPGYEYTGYSWGMVIDLNACIGCGACVVACQAENNIPVVGREQVRR
                     GREMHWIRVDRYYEGDPNDPAIHHQPVPCMHCENAPCELVCPVGATVHSDEGLNQMVY
                     NRCVGTRYCSNNCPYKVRRFNFLQFADWVTPSLKPMRNPDVTVRSRGVMEKCTYCVQR
                     INAAKIQAEEEDRVVRDGEIVPACAQACPAQAIIFGNINDPASQAARWKAEPRNYGIL
                     EDLNTRPRTTYLAEVRNPNPEITGERHGGI"
     gene            complement(3285..3935)
                     /locus_tag="VFA54_11715"
     CDS             complement(3285..3935)
                     /locus_tag="VFA54_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZS51523.1"
                     /translation="MAQIFHRSTNTIARVTILGGVFILIAVSWILYLFYRSDYMTGAR
                     VVRDQPVPFSHKHHVSDDGIDCRYCHTSVEDSSFAGIPPTETCMSCHSQIWAGAAMLE
                     PVRASFRNNQPLEWTRVHDLPDYVYFDHSIHINKGIGCSTCHGRVDQMPLTWRENTLL
                     MEWCLDCHRDPARYVRPRQDVFQMDYTPPADQRSLGRKLVQEYKIRSLTDCYTCHR"
     gene            4092..6782
                     /locus_tag="VFA54_11720"
     CDS             4092..6782
                     /locus_tag="VFA54_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HZS51524.1"
                     /translation="MPADSEFPAAGQVSSASRRPQGKLRVFLGYAAGVGKTYAMLEAA
                     RRRKQEGREPLIACVDTHGRPEAANLLAGLKVLPPPSAGAGIDVDFILARRPPLVVID
                     DLARANPPSARREKRYQEVEDLLEHGIDVYTTLNIAQIESLKATVEEITGRAPANTVP
                     DRILQEAEEIRLIDIHPNELLERLKQGKVNVADHGGSGVRRFLRPDELLALRELAFHY
                     TARRVREQIAARLQSDSLPATQLPRECVLVCVGLSDSNARLLQVGQQMAKGLEAELAA
                     IYVETRPFAGLSEPDKHRLSETLRLASELGATVVTVRGRSVVEEVLRYSRAHHVVRIV
                     VGRSRRFTLAAALRRSVSDQMVRRSEEAQVYVIDPSAPASGGVRRQPRRVRSNWESYA
                     LSLGLILLVTIFGWWIREILRPVNLQVLYLLAVVVSALRWGRRAAIFSAALGTFVFDF
                     VFVPPQLSFAITDVQFLVTLIGLLAVALVTGTLAGRVREQIEAARNREAYSASLYSLS
                     RSLATTRSLDRILEVIAYHIGETFERPVVILLPADGRLSAMFESPEFPFPQEERAAAE
                     WVFQHGRQAGPGTGMFTETATRYLPLTTSRGVVGVLGVKAAGPGERWLSAEQHRLLEA
                     FANQAALASERALLEEKARHAQLLEQTDKLQKSLLNSISHDLRTPLASITGALSSLSG
                     DSAVLDEATRRELLDTAQEEARRLNRLVGNLLDMTRLEAGAMRVKIEPCDVQDVIGAA
                     LAQLGEAARKRKIVVETQPDLPLAPMDFVLITQTLVNLLDNALKYSPAEEAICIQARA
                     SEQDLEVLVADRGIGIPPGEVGRIFERFRRQSRSAVQQGAGLGLSICKGFVEAHGGRI
                     WAERRPEGGSIFGFTLPRFARKAPAVEMANEPAEVASSGD"
     gene            6748..7443
                     /locus_tag="VFA54_11725"
     CDS             6748..7443
                     /locus_tag="VFA54_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS51525.1"
                     /translation="MNQQKLRVLVIDDEPAIRRFLRVSLNANGYATVEAASGEEGLQL
                     VPSVRPDLIILDLGLPGISGTEVARSLREWSQTPIIVLSVREQEADKISALDAGADDY
                     LTKPFGMGELLARMRAALRRAAAHENGPIFSTGDLWVDLPRREVRCGEHKVQLTPTEY
                     DLLKALIAHAGKVLTHRQLVKEVWGGVSYEDELHLLRVNISNLRRKIEPDPTRPRYVV
                     TEPGVGYRLHTDS"
     gene            7665..8318
                     /locus_tag="VFA54_11730"
     CDS             7665..8318
                     /locus_tag="VFA54_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS51526.1"
                     /translation="MNGLCLRNTPDNLSPDVPDEEVLARVRSGQTEFYEILMRRHSPQ
                     LRRVARRILQNDADVEDLMQEAHFNALRHLDQFAGRSSFSTWLIRIAIHGAMTKLHRQ
                     PRFLDLDSPPLSANAGMLLASRGRDPEKQVLDRELRRLLRLALDSLPRRYRSVFELRE
                     IEGMNTAQTAALLRISETCVKTRLHRAKSLLRSKLKRRLAPARGAHKSFHWPRCLAA"
     gene            8476..9408
                     /locus_tag="VFA54_11735"
     CDS             8476..9408
                     /locus_tag="VFA54_11735"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HZS51527.1"
                     /translation="MRRVEKQAKEILVLFLVAAILAPGLLVAQYPPYGTPPPPSPYGP
                     PPPLLPAAELDRLVAPVALYPDPLLTQVLTASTFYDQIPQAATWADQHSYLHGDQLAA
                     AISADNLPWDPSVIALLPFPSVLGMMARDMAWTTQLGNAFLSQPGAVMDAVQRLRQQA
                     LSYGYLVNGPQVQVVRSGPYIEILPVSPGVICVPVYNPAVVFVPRRPGLHVTISFGAA
                     VLVGASRPWGWAEPHFVWPSRTIIIDNHPWQRTWANRREYVHPYRIAPLPAARRGPPV
                     ERHELRGRAPAEKGHADRGQNGRDRGHEDEDRRR"
     gene            9561..>12091
                     /gene="polA"
                     /locus_tag="VFA54_11740"
     CDS             9561..>12091
                     /gene="polA"
                     /locus_tag="VFA54_11740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZS51528.1"
                     /translation="MPENPARLFLIDTFGLIFRAYHARARTGAPAMRTRSGMATEAVY
                     IFNNMLRKILAEHHPEYVVAVFESLAPTFRDEIYDQYKANRATTPSELLQQIPYIRRL
                     LAALGIPVIEHDRFEADDVIGALARCSAAKGLEVYIVSSDKDLLQLVGDHIFVLDPSK
                     DNLIYDDRKVEEYMGVLPGFVADLLALKGDAVDNIPGAPGIGDKGARELVKRFGTVEQ
                     ILDHAAEVERKTYRNSLLENRDRILLSKKLAVVDTSVPVQLDLEAVKPAPIDLARLSA
                     LYGELEFHSLLKDLPPVPQAPADYAGLETEEEVRAWLGDAPARGATVALSLDPLSGAE
                     QIQGCRVGISVREAEARLAPLPAVRKLLEDAAVSKAVHDYKRAWGLLDSHGVNPAGVK
                     HDTRLYTYLLDPSRSNYSLGESVLRRFGRPLDPGPAPAADFTWRLAGILAPEIDAAGL
                     REVYENVELPLAPVLVRMERAGVAIDLGVLSRLSAEMELRLSALTAEIHRLAGREFNI
                     NSPQQLSKVLFEDLKLPMAGRSSKTKAPSTAAAVLEALAPDHEIVRKILDYRELAKLK
                     GTYVDALPALVNPSTGRLHTTFDQCGSATGRLSSADPNLQNIPIRTELGRQIRAAFIP
                     RPGWRLVVADYSQIELRVLAHVSQDAVLIQAFRSGEDIHTRTAAEVLGVPPLLVGPEE
                     RRRAKAINFGIVYGLSAFGLAAQLGISRTDAQRYIDGYFSRYAGVKRYIDSVISEVRR
                     TGFTRTLFGRRRAIPDINSPNPPARNFAERTAVNSPIQGAAADLIKLAMISIDRRLRQ
                     EKFRAEMVLQVHDELLFEAPPEEVDSLGSLVKHEMESVRKLDVPLL"
BASE COUNT         2105 a         4055 c         3606 g         2325 t
ORIGIN      
        1 ccggcatggc cgatcccgat ccaccacacg aaattcacga tggcgaagcc ccaagcgacg
       61 ggaatgttga tcccccagat ccccacgccg gtcgccagca ggtacgccac cgcgccgaag
      121 agcagcaaca caagcagaaa cgcgataccg aaacccagcc accaggccag aggagggcgc
      181 cggctgagga caatcgcgct gatcttgtcc gttaccgagg cgtaggtata gcccggctcg
      241 attaccggga ccgcgtctgc cgtaattggt ctgccgcctt cagattccgc catgtctttc
      301 gcccgttatc tccgggttgg gatttcgcac ttcggccaga tacgtcgtgc gcggccgcgt
      361 gttcaagtct tccagaatcc cgtaattgcg cggctcggct ttccaccggg ccgcctggct
      421 cgccgggtcg ttgatattgc cgaaaatgat ggcttgcgcc gggcaggcct gggcgcacgc
      481 cggcacgatt tcaccgtcgc gcacgacacg atcctcttcc tccgcctgga tcttcgccgc
      541 gttgatgcgc tgcacgcagt atgtgcactt ctccatcact ccccggctgc gaacggtcac
      601 gtccgggttt cgcatcggct tgagactggg cgtgacccaa tcggcaaatt ggaggaaatt
      661 gaagcgcctt actttgtaag ggcagttatt cgagcagtag cgcgtcccga cgcagcgatt
      721 atagaccatt tgattgaggc cctcgtcgct gtgcaccgtg gcgcccaccg ggcataccag
      781 ttcgcacggc gcattttcgc aatgcatgca cggcacgggc tggtgatgga tcgcggggtc
      841 gttcggatcg ccttcgtagt accggtccac ccggatccaa tgcatttccc gccctcgtct
      901 cacctgctcc cgccccacca cgggaatgtt gttttcggcc tggcacgcca ccacgcaagc
      961 gccgcacccg atgcacgcat tgagatcgat caccatgccc caggaatagc ccgtgtactc
     1021 atagccgggg tagagcgaaa aatccggctt cgggcgctcc tccacctcct gagcgaattg
     1081 cggccttttc tcgtactcgg cggccgtcgc cgatcggatc aaatgccggc cttccatgct
     1141 gaagtggttc tgcgtcgaga ccaggtccga gcgcaaaccg gtcctggcta tctccacgcc
     1201 atagccgaac cacggctgat ccgagtggcg cagcgcataa gcgttgaatc ccgcgtcgct
     1261 ccccggtctg ccgctccggc ggccgtagcc gaggtgcacg gttatggagt catccgcgtg
     1321 gccgggcatt acccaaaccg gcgctcgcac agtggctcct cgaaagcgca gatcggccac
     1381 gtccgaactg tccaggccca ggcgcgccgc cgtcgccggg ctcatcatcg ccgcgttctg
     1441 ccaggtgagc ttggacatcg gcttcggcag ctcctggagc caaccgttct cgctgaatcg
     1501 tccgtcccag atcgtcgggt cgggccggaa aacgatctcc agtcctgcgg cgcgcggcgg
     1561 ggcgcctatt tgggccggga atccggcctt caggttcacg cgcttcgggg gcagcgccgt
     1621 gccggcgatc aggccgtcgt gcaaggcctt ttgccagtcg tcgtcgaagc tctcccagcg
     1681 ccgctgaacc ttccagtagt catggacaag gtcgtgcgtg gcggccgccg gctgccccag
     1741 cagcgccgcc agcacttcgt acgcggattt cccctggtac aacggcgcga tcagcggctg
     1801 gatcatgctc acggtcccgt cataggcgcg ggcatccccc caagactcca gaaagtgcgc
     1861 ttccgggatg tgccagttgc acagtaccgt ggtctcgtcg tcataaaggc ttaactgcgc
     1921 tttgaagcgc actcgggaaa gagcggcggc gaagcccaga tcgcgcggcg cgtagaacgc
     1981 gggattgccc cccaatatga tcagcgcgtc cacgcggccg gcttgcatat cttccaccag
     2041 ttcgcgcagc gattcggtct gatcaaccgg attcgcttcc accggatccg tatagaccac
     2101 ggtctcccct tcgtttccca ggatgcggtt catcgcgtgc gccagcgcgt gcaccgccgg
     2161 gggctgctga gctccggcga ttaccaggct tgatccgcgg ttgcgctcga ggtcgcgggc
     2221 cagtgcgtcc acaaaagccg tgttggctcg ggcttccccg gccgcgcccg caatgcccag
     2281 ccgccgcccg agttcgcggg ccaagtcttc cacttcgctt gcgcgagccc gcaagcgatg
     2341 gtcggccata gaccctgtga tcgaaggaga aggctcgatc gcgtacaggc gattcaggga
     2401 ggctcgcccg ccgctcacgc gccgccgggc cgcgaaatcg tgcgtgtagc ggatgctgcc
     2461 cgggccttcc cagagaaaat cggcgtcgag cgagagaatc acatccgccc gctccacacg
     2521 gtacacggga ttgacgtctt cgccaaaggc gagcagcgcc ccggcacggg cattgtcgcg
     2581 cgacaccggt tcgtattgat gccatttggc cgccgggaac tcggccagca ccgagcggat
     2641 caggcctgcc aggctcggtg aggtgatcgt ttccgtcagc aggcgaagcc ccgcgccccg
     2701 cttgctgcgc tgctcctcgc gcaggccgtt gagcgccgcc gcgaaggccg gccaagtgct
     2761 gatgcgcccc tcccgagtca ctacctggga gcgattcgga tcatacaggg tgagcaccga
     2821 agcctgcccg aatgcgtccg tggcccccag gctcgccggg tgctccggat tgccttccgc
     2881 tttgatcggc cggccggtgt ggctcttgac cagaagccct ttggcgaagc cgcccagcac
     2941 catcgacgtg gcaaaaaaca acggcttgcc cggcgtcact tcctccggcg ctcgcacata
     3001 cggcacaacc ttccgcatgg gcggaggcgg ccaatcgggc cggccggctc cctgcggcga
     3061 gcatgcggtg aggccggcaa gcgccagcga agcgcccatc catttcagca gttcccggcg
     3121 gctcaccgga tcggcccagc tcggcgcctc ttccggaaac tcgctccgca aaaactgcaa
     3181 aaattcttcg cttccggtta gttcctccaa gctccgccag tgcctcggtc cggatgcgag
     3241 gcgcccgcgg gcaagggaaa aggcgtcggt gtggcgttcg tccttcatcg gtggcaggtg
     3301 tagcagtcgg tcaagctgcg aatcttgtat tcctgcacga gcttccgccc caggctgcgc
     3361 tgatccgccg gcggcgtgta atccatttgg aaaacgtcct ggcgcggcct cacgtaccgc
     3421 gccggatccc gatggcagtc caggcaccat tccatcagca gcgtgttttc ccgccatgtc
     3481 aacggcatct ggtccacgcg cccgtggcag gtggagcatc cgattccctt attgatatga
     3541 atgctgtgat cgaagtagac gtaatcggga agatcgtgca cccgggtcca ctcgagcggc
     3601 tgattgttcc ggaagctggc ccgcaccggc tcgagcatcg cggcgccagc ccagatctgg
     3661 gagtggcagc tcatgcacgt ttcggtcggc gggatcccgg cgaacgagga gtcttctacc
     3721 gaggtgtggc agtaccggca atcaatgccg tcatcgctca catggtgttt gtgactgaat
     3781 ggtaccggct gatcgcgaac cactcgggcc ccggtcatat aatcggaccg gtaaaacaga
     3841 tacaaaatcc agctcacggc gatcagaatg aacacgcctc ccaggatcgt tacccgtgca
     3901 atcgtattcg tgctgcgatg aaaaatctgc gccatgcagg ctgctgggca gaactaaccc
     3961 accccgctta gaggcgaact tgtcgacacg gttacagttt tttttagaac cgccgccgca
     4021 ggccgccccc gccccgggcc gtgcgatacg ataaagaacg acagtggcag cagttagggt
     4081 agggaccgta tatgccggcc gactctgagt tcccagccgc cgggcaggtc tccagcgctt
     4141 cccgccggcc ccaaggtaag ctccgtgttt ttctgggata tgccgcaggc gttggtaaga
     4201 catacgccat gctggaagcc gcccgccgcc gcaagcagga gggccgcgag cctctgatcg
     4261 cctgcgtgga cactcacgga cgccccgaag cggccaacct tctggccggc ctgaaggtct
     4321 tgccccctcc cagtgcggga gccggcatcg atgttgattt cattctcgcc cgccgcccgc
     4381 cgttggtcgt gatcgacgac ctggctcgcg ccaacccgcc ttccgcccgc cgcgaaaagc
     4441 gttatcagga agtcgaggat ctgctcgaac acggtatcga tgtttacacc acgctgaaca
     4501 tcgcgcagat cgaaagcctt aaagccacag tggaagaaat caccggccgt gcccccgcga
     4561 atacggttcc cgaccggatc ctgcaagaag ctgaagaaat ccggttgatc gatattcatc
     4621 ccaacgagct cctggaacgc ctgaaacagg gaaaagtcaa tgtcgccgat cacggcggct
     4681 cgggagtccg gcgttttctc cgtcccgacg agctgctggc gctccgggaa ctggcgttcc
     4741 attacaccgc caggcgggtc agagagcaaa ttgccgcccg gctccagtct gactcgctcc
     4801 cggccaccca gttgccgcgg gagtgcgttc tcgtatgcgt cggattgagc gattcgaacg
     4861 cccggttgtt gcaggtcggc cagcagatgg ccaaaggcct ggaagcggag ttggccgcga
     4921 tttacgttga gactcgcccc ttcgccggcc tttccgagcc ggacaagcac cgcctttctg
     4981 aaactctccg gctcgcttcc gagcttggcg ccactgtcgt aactgtccgc ggccgctcgg
     5041 tcgtggagga agtcttgcgc tacagccggg cccatcatgt cgtccgcatt gttgtcggca
     5101 gatcgcgccg gttcacgctg gccgccgcgc tacgccggtc ggtctccgat cagatggtcc
     5161 ggcgcagcga agaagcccag gtttacgtca tcgatccttc cgcgcccgcc tccggcggcg
     5221 tgcgccgcca gccgcgccgg gtaaggtcta actgggaatc ctacgctctg agccttggat
     5281 tgatcctgct ggtcaccatt ttcggctggt ggattcgcga aattctccgg ccggtgaact
     5341 tgcaggtgct ctatcttctg gctgtagtgg tgagcgcgct ccgttgggga cggcgagccg
     5401 ccatcttcag cgccgctctg ggcacgtttg tctttgactt tgtctttgtc cctccccaac
     5461 tgagcttcgc cataacagac gttcagttcc tggtcacctt gatcggcttg ctcgccgtcg
     5521 cgctggtgac ggggaccctg gccgggaggg ttcgggaaca aattgaagcg gcgcgaaatc
     5581 gggaagccta ttccgcttcg ctgtactcgc tcagccgctc gctcgccacc acgcgctctt
     5641 tagatcgtat tctcgaggtc atcgcttacc acatcggcga aacctttgag cgccccgtcg
     5701 tgatcctgct gccagcggac ggccgcctga gcgcaatgtt tgaaagcccg gagttcccgt
     5761 tcccgcagga agaacgagcc gccgcggaat gggtcttcca gcatggccgc caggcagggc
     5821 ccggaaccgg catgttcacc gaaactgcaa cccgctatct gcctctcacg acctccaggg
     5881 gagtggtggg cgtgttggga gtcaaagcgg ccggcccggg cgagcgctgg ctctctgcgg
     5941 agcagcatcg cctcctcgag gcttttgcca accaggcggc gctggcttct gaacgcgctc
     6001 tcctcgagga aaaagcccgc catgctcagt tgctggagca gaccgataaa ttgcaaaaat
     6061 cgcttctcaa ctccatttcc cacgatctgc gaactccgct ggcttctatc accggcgctc
     6121 tcagcagcct ttccggggac agcgccgtgc tggatgaggc gacccggcgc gaattgctag
     6181 acaccgccca ggaagaagcc cgccggctca accggctggt tggaaatctg ctggatatga
     6241 cgcgcctgga agccggcgct atgcgggtca aaatcgaacc ttgcgatgtg caggacgtga
     6301 ttggagcagc tttggcccaa ctcggcgaag cagcccgaaa acgcaagatc gtggtcgaaa
     6361 cccagcccga tcttcctctg gccccgatgg attttgtcct gatcacccag accctggtca
     6421 acctgctcga caatgcgctc aagtactctc cggccgagga agccatttgc attcaggcgc
     6481 gggcgtccga gcaggatctg gaggttctgg tggctgaccg cggcatcgga attccaccgg
     6541 gcgaagtcgg ccgaattttc gagaggttcc ggcggcaatc gagatccgcc gtgcagcaag
     6601 gcgctggtct cggcctgtct atctgcaaag gttttgtcga ggcgcacggc ggccggattt
     6661 gggcggagcg gcgtccggaa ggcggctcga tattcggctt tacgcttccg cggtttgcca
     6721 ggaaagcgcc tgcggtggag atggcgaatg aaccagcaga agttgcgagt tctggtgatt
     6781 gatgacgagc cggccatccg gcgttttctt cgcgtctcgc tgaacgcgaa cggctacgcg
     6841 accgtggagg ccgcgagcgg cgaggaaggc ctgcaattgg tcccctccgt gcggcccgat
     6901 ttgatcattc tggatctcgg tttgcccgga atcagcggga ccgaagtcgc acgctccctc
     6961 cgggagtgga gccagacgcc gattatcgtc ctctccgtgc gagagcagga agccgacaag
     7021 atctcggcgc tcgacgccgg cgccgacgac tatctgacca agcccttcgg catgggagag
     7081 ttgctcgccc gaatgcgcgc cgccctgcgg cgagccgccg cgcacgaaaa tgggcccatt
     7141 ttcagcaccg gcgacctttg ggtcgatctg ccgcggcgcg aagtgcggtg cggcgagcac
     7201 aaagtgcagc tcactcccac cgagtacgac ctgctcaagg cgctgattgc gcacgccgga
     7261 aaagtcctca cgcaccgcca gctcgtcaaa gaggtctggg gcggcgtctc ttatgaagac
     7321 gagctccatc tgctgcgggt caacataagc aatctgcgcc gcaaaatcga gcccgatcct
     7381 acacgccccc gctatgtggt caccgagccc ggcgtgggat accgcctgca taccgattcc
     7441 tgacccccgg tgaaaacacc tccggcataa atcaggggcc gccaacgtgg aagtctgcct
     7501 gcgcccgctt cttccccgcc gcatgtctgc aactccttgc tcttgatttt ttcttgaccc
     7561 gccctcgcgg gtttttgatc ccttctttat gcgactttcc ttaaattgga tgtgttggtt
     7621 tccgcgggtt gaattcgcag caggaaagac gggaggaatg ggacgtgaac gggctttgcc
     7681 tgcgcaatac gccggacaat ctgtctccgg acgttcccga cgaggaagtg ttagcgcgcg
     7741 tgcgctccgg gcagaccgag ttctatgaaa tcctgatgcg ccgccacagc ccgcagctcc
     7801 ggcgcgtggc tcgccggatc cttcagaacg atgcggacgt cgaggatctc atgcaagagg
     7861 ctcacttcaa tgcgctccgt catcttgatc agttcgccgg ccgctcgagt ttttccacct
     7921 ggctcatccg aatcgcgata cacggcgcga tgaccaagct gcatcgtcaa ccgcgatttc
     7981 tggatctcga ttctccgcct ctcagcgcca atgccgggat gctgctggcc tcgcgaggac
     8041 gtgatccgga aaagcaggtt ctcgaccggg aactgcgccg cttgctgcgg ctcgctctgg
     8101 actcgttgcc gcgcaggtac cgttcggttt tcgagctccg ggaaattgaa ggcatgaata
     8161 cggcgcaaac cgctgcgctt ctgcggatca gcgaaacctg cgtcaaaacc cgcctgcacc
     8221 gcgccaagag cctgctccgg tccaagttga aaaggcgctt ggcgcctgcc cgcggagccc
     8281 acaaatcgtt ccactggccg cggtgcctgg ctgcctaagc gcatttcggc tcccgatctc
     8341 caacagtccc agcgttgccg ctcgtcctcg ctgttccctc gagacgcaat ctttgtctat
     8401 gtctccggta acgcggaagc cttctggggc atccaaaaca gagggccgga atttctttgg
     8461 agtggaggcg gttccgtgcg gcgagtggag aagcaagcga aggaaatttt agtccttttt
     8521 ctagtcgcgg cgattctggc gccggggctg ctggtggctc agtacccgcc ttatggaacg
     8581 ccgcccccgc cgtctcccta cggacctcct ccgcctctgc tcccggccgc cgagttggat
     8641 cgcctggtcg cccctgtcgc gctctatccc gacccgctgc tgacccaagt cctcaccgct
     8701 tccacgtttt atgatcagat tccccaggcc gctacgtggg ccgaccaaca ctcctattta
     8761 cacggggacc agctcgcggc cgccatctct gccgacaatc ttccgtggga tccgagcgtg
     8821 atcgcgctgc tcccgtttcc ctcggtactc ggcatgatgg cgcgcgatat ggcgtggacc
     8881 acgcagttgg gcaacgcgtt tttgtctcag ccgggggcgg taatggatgc cgtccagaga
     8941 ttgcgccagc aggctctcag ttacggctac ctcgtcaacg gtccgcaggt ccaggttgtg
     9001 agatccgggc cgtatatcga gattctgccc gtcagccccg gtgtgatctg cgttcccgtc
     9061 tacaaccccg ccgttgtttt tgtgccgcga aggccggggt tgcatgtgac catctcgttc
     9121 ggcgcggctg tcctcgtggg agcctcccgg ccctggggct gggcagagcc gcactttgtc
     9181 tggccctctc gcacgatcat cattgacaat cacccttggc agcgcacgtg ggccaatcgc
     9241 cgcgaatacg ttcatccgta tcgcatcgcg ccgctgccgg cggcgcgacg cggcccgccc
     9301 gtcgagcgcc atgagctgcg cggccgcgcc ccggcggaaa agggccacgc cgatcgcggc
     9361 cagaatgggc gcgatcgcgg ccacgaggac gaagatcgcc gccgctagcc gttttccccc
     9421 tccgcagcgc cgcggcccgg ctgaaattct ctcctcgctg gccgagccgc ctcgtccaga
     9481 cttccgcaat atgacgaggc gccgcgcctc ccgagcccgc cggctaatcc acacccgggc
     9541 ggtggtacta agatgaggag atgcctgaaa atcctgcccg gctattcctg attgatacgt
     9601 tcggcctgat ttttcgcgcc tatcatgccc gcgcccgcac gggagcgcct gccatgcgca
     9661 cgcgctccgg catggccacc gaggccgttt acattttcaa caacatgctt cggaagatcc
     9721 tggccgagca ccatccggag tatgtcgtcg ccgtgttcga aagcctggcg cctacatttc
     9781 gcgatgagat ttatgaccag tacaaagcga atcgcgccac tacgcccagc gagctgctcc
     9841 agcagatccc ctatatccgc cgcctgctcg ccgccttggg catccccgtc atcgagcatg
     9901 accgctttga agccgacgac gtgatcggcg ctctcgcccg ctgctccgcc gccaagggcc
     9961 tcgaagtcta catagtttcg agcgacaagg acctcctgca attggtcggt gaccatatct
    10021 tcgttctcga cccatccaag gacaacctca tctatgacga tcgcaaggtc gaggagtata
    10081 tgggtgtgct tcccggcttc gtcgccgatc tgctggccct caagggcgac gccgtcgaca
    10141 acataccggg cgcccccggg atcggagaca agggcgcgcg cgagctcgta aagcgctttg
    10201 gaaccgtcga gcagatcctc gaccacgccg ctgaggtcga acgaaaaacc tatcgcaaca
    10261 gtttgctcga gaaccgcgat cgcatccttc tcagcaagaa gctcgcggtc gtggatacca
    10321 gcgtcccggt ccagctcgac ctggaggcgg tcaagccggc gcccatagac cttgccaggc
    10381 tctccgcgct ttatggcgag ctcgagtttc acagccttct caaagatctt ccgccggttc
    10441 cccaggcccc tgccgattac gccggcctcg aaaccgaaga ggaggtgcgg gcctggctcg
    10501 gcgacgcccc tgcgcgcggc gcgaccgtgg ccctgtcgct ggacccgctg tccggcgcgg
    10561 agcagatcca gggctgccgc gtcggcattt ccgttcgcga agccgaggcg cggcttgcgc
    10621 ccttgcccgc ggttagaaaa ttgctcgagg acgccgccgt ttcaaaggct gttcacgatt
    10681 acaaacgcgc ctgggggctg ctcgactcgc acggcgtcaa tccggccggc gtgaaacacg
    10741 atacccggct ctatacatac ttgctcgacc catcccgctc gaactactcc ctgggcgagt
    10801 ccgtcttgcg gcgctttggc cgccccctcg atcccggccc ggcccccgcc gccgacttca
    10861 cctggcgtct cgccgggatc ctggccccgg aaatcgacgc cgccggcttg cgcgaggtct
    10921 acgaaaacgt cgagctgccg ctcgcgccgg ttctggtgcg gatggagcga gccggcgtcg
    10981 ccattgacct cggcgtgctt tcccggctct ctgccgagat ggagctccgc ctcagcgctc
    11041 tcaccgccga gattcaccgg ctggcaggcc gggagttcaa tatcaattca ccgcaacaat
    11101 tgagcaaggt tttgtttgaa gatctcaagc tgcccatggc cggccgctcc agcaagacga
    11161 aggctccctc taccgccgcg gccgtgctcg aggcgctggc ccccgatcac gagatcgtca
    11221 gaaagattct ggattaccgc gagctggcca agctcaaggg cacttatgtg gacgctttgc
    11281 ccgccctggt gaatccgtcc accggccggc ttcacaccac ctttgatcag tgcggctcgg
    11341 ccacgggccg cctctcttcg gctgatccga atctgcaaaa cattcccatc cgcacggaat
    11401 tgggccgcca gatccgcgcc gccttcatcc cccggcccgg ctggaggctg gtcgtcgccg
    11461 actactccca aatcgaactg cgcgtgctgg ctcacgtttc ccaggatgcc gttctgattc
    11521 aggctttccg cagcggcgag gatatccaca cccgcaccgc cgccgaggtc ctcggcgttc
    11581 cgcctttgct ggtgggcccc gaagagcgcc gccgggccaa agccattaac ttcggaatcg
    11641 tctacgggct gagcgccttc ggcctggccg cccagctcgg catttcccgg accgacgccc
    11701 agcgctacat cgacggctat ttctcgcgct atgcgggcgt gaaaagatat atcgactccg
    11761 tcatctccga ggtccggcgg accgggttca cgcgaacgct gttcggccgc cggcgcgcca
    11821 ttcccgatat caactcgccc aaccctcccg cgcggaattt cgccgaacgc acggcggtca
    11881 attcgcccat ccagggcgcc gccgccgacc tcatcaagct cgccatgatc tccattgacc
    11941 ggcgcttgcg ccaggaaaaa ttccgggccg agatggtgtt gcaggtccat gacgagctgc
    12001 tgtttgaggc gcccccggaa gaggtcgatt ccctgggatc tctggtcaag cacgagatgg
    12061 aatcggtgcg gaagctcgac gtgcccctgc t
//
LOCUS       DASREK010000224         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1756784, whole genome shotgun sequence.
ACCESSION   DASREK010000224 DASREK010000000
VERSION     DASREK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756784"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VFA54_11745"
     CDS             complement(<1..1375)
                     /locus_tag="VFA54_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51529.1"
                     /translation="MHKAFRMRALCFLWLAAGALPVLAQHDMPGHAGHDMGQMAGHDM
                     SHMTTHSPGANGDPVSLSAMDQASGTSANPASSPMNMMEARIGRWDFLFHGVAFISDI
                     QQTGPRGGDKFASMNWFMGSAEHQAGRGSIQFRLMLSLDPATVTDRRYPELFQTGETA
                     FGKPIVDGQHPHDFIMESSVQYRRPVGEKSVLNLYLAPVGDPALGPAAFPHRVSAAEL
                     PQAPLSHHWQDSTHIANEVVTASLTRGIFRWEASGFHGAEPNENRWNIDHGAIDSWAV
                     RAWVNPAPNWSGQVSVGRLHHPEILEPDDVVRATASLTYNKPLTGGCWATSLIWGRNH
                     KTVAQRNTNSYLLESVYQFRHTNYLTGRVEMVDKDELFQAQPQLRDRLEETAGSTFRI
                     IPYTLGYTRDVSLLPGLTTGFGANVTLYRVPAVIQRYYGEHPVSVIFYMRVRLAGSGP
                     MQHSSHRS"
     gene            2150..3073
                     /locus_tag="VFA54_11750"
     CDS             2150..3073
                     /locus_tag="VFA54_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS51530.1"
                     /translation="MNARPLIHAISLVPMTPAQRSRLERLGNLCYFDAVLGDSHLIEK
                     CRGAEILVITPRLHMDIVPGLDRCRFISVQGAGTDALNVAAARQRGILVSNVPDFCAD
                     AVAEHAFALLLGVAKRIEEGRSVLQGGKWRTALAYETVGLKGKTLGLFGHGKIGRRIA
                     DIGRAFGMNVLFTTRSPLSTPFQQLLAQSDFLVIAAPATPETRGRFSAPAFAAMKPGA
                     ILVNVSRAALVDESALLAALDAGRLAGAALDVFSIEPPPPADPVLRHPRLLVSPHVAW
                     GTQDAVCRLVDLSIANVEAFLAGSPVNLVSA"
     gene            3177..4421
                     /locus_tag="VFA54_11755"
     CDS             3177..4421
                     /locus_tag="VFA54_11755"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51531.1"
                     /translation="MKIRNRVLTLLFSLTVITYLDRVCIGTMAGLISRDLGLTPQQMG
                     AVFSAFILGYVLFEVPGGWLADRYGARLLLPRIVAWWSLFTAATAFAWNFTSMVVIRF
                     LFGAGEAGAFPACSTVVLRWFPKSERGRANGLLLMGSRLGGAVAPALVIALMRSFGWR
                     KAFLAFAAFGIVWGVIWFWWYRNEPEKHRSVRAEELREIRGDAGIPTAASLPWKQMWR
                     SRNLWALCAMYSGYTYGLYFYLTWLPTYLRQQRGMSWAGVGVGAALPLLMGAAANTAG
                     GYWTDSLVRRFGLRWGRRIPAISGLLASAVLLAAAALTPANAPAVAALAASFGAADLI
                     VAVCWATCIDLGKQNAGAIAGVMNSLGQIGGMISPFLLGWLFEKSGSWTLPLLISAGY
                     YAASAALWLVIDPEDDRYLAAS"
     gene            4658..>4956
                     /locus_tag="VFA54_11760"
     CDS             4658..>4956
                     /locus_tag="VFA54_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/galactose cotransporter"
                     /protein_id="HZS51532.1"
                     /translation="MTLAPIDYLILAIYFAFVLGIGYWLRAKVHSSEEFFLSGRSIPL
                     WITGLAFVAANLGAQEVIGMCASGAKYGIMTAHFYWLGAVPAMVFVGVFMMPFYYG"
BASE COUNT          846 a         1492 c         1572 g         1046 t
ORIGIN      
        1 acgatctgtg gctggagtgc tgcattgggc cgctgcccgc cagccgcacc cgcatataga
       61 aaatcacgct caccgggtgc tctccataat agcgctggat caccgccggc acgcgataca
      121 gagtaacgtt ggccccaaaa cccgtggtga ggcccggtag gagcgagaca tcgcgcgtgt
      181 agccgagcgt ataagggatt atccgaaagg tcgatccggc ggtctcttcg agacggtctc
      241 tgagctgagg ctgcgcttgg aaaagttcgt ctttgtcgac catctcgacc cggcccgtca
      301 gatagtttgt gtgtcggaac tggtaaaccg attcgagcag ataggaattg gtgttgcgct
      361 gcgcgacggt cttgtggttt cgcccccaga tgaggcttgt cgcccagcaa ccgccggtca
      421 gcggcttgtt gtaagtgagc gacgcggtcg cgcggaccac gtcatcgggc tcaagaatct
      481 cggggtgatg caggcgtcct acggagacct gtccgctcca attaggagcc ggattgaccc
      541 aggcccggac cgcccaggag tcgatggctc cgtgatcgat gttccaacga ttctcgttgg
      601 gctctgcgcc gtgaaaaccg ctggcttccc agcgaaaaat cccgcgcgta aggctggcgg
      661 taaccacttc attagcgatg tgggtagaat cctgccagtg atgagagagc ggagcttgcg
      721 gcagttccgc cgccgagact cgatgcggga aggccgccgg ccccagcgct ggatcgccca
      781 caggggccag gtacaagttc aaaacactct tttctccgac gggccggcga tattggacgc
      841 tgctctccat gatgaaatcg tgcggatgct ggccgtcaac gattggcttg ccaaacgcgg
      901 tttctcccgt ttgaaacagc tccggatagc gacgatcggt tacggtcgcc ggatcgagac
      961 tcagcatcag gcggaattgg atgctgccgc gccccgcctg gtgttcggcg ctgcccataa
     1021 accagttcat ggatgcgaac ttgtcgccgc ctcgcggtcc ggtttgctgg atatcgctga
     1081 taaaagctac gccgtgaaac aggaagtccc atctgccgat gcgcgcctcc atcatgttca
     1141 tcggcgacga agccggattg gcgctcgttc cggaagcctg atccatcgcg gaaaggctca
     1201 ccggatcccc gttggcgccg gggctgtgcg tcgtcatgtg gctcatgtcg tgcccggcca
     1261 tttggcccat gtcgtggccg gcgtgtcctg gcatatcgtg ctgcgcgagg acgggtaacg
     1321 cgcctgcggc aagccacaag aaacagagag ctcgcatcct gaatgccttg tgcataggaa
     1381 aacaatcctt ccatgtagaa tccctgcgtc ctggcgggcg gcgtcgtttc ggaaaggccc
     1441 gcaggatgtt caaccaacgc ggctggcatc ctcgtcgccc tgtcaaaaaa aggctttccc
     1501 gcaggtcata cgggcgaagg cttttcctaa ggatgccttg gggcgaggcc gggctaaatt
     1561 ctcaatacac tgattcgagg taaataaggc aggccggccg agagagctct ctcagccggg
     1621 aatgaagcac tccccgaagg cgcgccttcc aagggatgcg acggcgcggc gacggagtaa
     1681 ggcgcgggaa cagtcttggg aataatcgaa gcagggagcc agtttcctcc tatccagaca
     1741 cccgggacgc aagaggagcc gccggcgggg gactgtttgg aatgcggcgc cggggtgttg
     1801 tggtggcaat ccgtcctgtt gtcgcgcaag ggctcgtggc cgcgggaaac gcactgccgc
     1861 gcggagcacc ccgtagcgca ctgagcgccc cggtacatca gcgcggacgt tgagaacacc
     1921 aacagaaaag ccatccaccg gcccataatc gtaacgatag ctcagtttcg aaaatgcggc
     1981 aacagggctc ggaagaacac cgcatcccat ccggcgaatg cggatcattt gatctccacg
     2041 aaatcccgca cggcgggcag gcgcctgcgg cgtcctcaaa ctgttctgag aatcccacat
     2101 cggtgtatcc tgcgataatg gccgggctgg cagcggcgcg gccacgcaca tgaacgcacg
     2161 gccacttatc cacgccattt ccttggttcc catgacgccc gctcaaagga gtcggctgga
     2221 gcggttgggg aacctttgct actttgacgc tgttctcggg gactcacatc tgatcgagaa
     2281 gtgcaggggc gccgaaattc tggtgattac gccgcggcta cacatggata tcgtcccagg
     2341 tttggaccgc tgccggttca tttccgttca gggggcgggc acggacgcac tgaatgtcgc
     2401 agccgcccgg cagaggggca ttctcgtttc gaacgttccc gatttttgcg ccgatgccgt
     2461 ggccgagcat gccttcgctt tgttgctggg cgtggcgaag cggatcgagg aaggacgatc
     2521 cgtgctgcaa ggggggaagt ggcggaccgc ccttgcttac gagaccgtcg gcctgaaggg
     2581 aaaaacgctt ggcctgttcg gacacggcaa gatcgggagg cgcatcgccg atattggccg
     2641 agcctttggc atgaacgtgc tgttcacgac ccgcagcccg ctgtccacgc ctttccagca
     2701 gctattggcg caaagcgatt ttctcgtcat cgccgccccg gccactccgg aaacccgcgg
     2761 acgtttcagc gctccggcat tcgcggctat gaagcccggt gcaattcttg tcaatgtgag
     2821 ccgggccgca ctggtggatg aaagcgccct gctcgcagct ctcgatgcag gccggctggc
     2881 tggggcggcg ctcgacgtat tttcgattga gccgccgccc cccgctgacc cggtcctgcg
     2941 ccatccgcgc ctcctggtga gtccccatgt cgcctgggga acccaggatg ccgtctgccg
     3001 tctcgtggat ctgagcattg ccaatgtcga agcctttctg gcgggatccc cggtgaacct
     3061 ggtctccgcc tagcggcgcc agcgcctgct ggccgttgct gaacgtggag agtttgtgac
     3121 atatacggcg ccgcagactg gcgtttccac ctgagctccg ctatcatcgc tgcttcgtga
     3181 aaatcagaaa ccgggtcctc acactcctct tctcgctcac cgtgatcact tatctggacc
     3241 gcgtgtgcat cggcacaatg gctggactca tatcccggga tctcggcctc acgccccaac
     3301 aaatgggagc ggtgttcagc gctttcatcc tcggctacgt gctgtttgaa gttccgggcg
     3361 gctggctggc cgaccggtat ggggcccggt tgctgctgcc gcgtatcgtg gcctggtggt
     3421 cgctgtttac cgcggcgacc gctttcgcct ggaacttcac gagcatggtc gtaatccgtt
     3481 ttctgtttgg ggcgggagaa gccggcgcat ttccagcttg ctcgacggtg gttttgcggt
     3541 ggtttcccaa atcggagcgc gggcgcgcga acggcctgct gttgatggga agccgcctgg
     3601 gaggggccgt agccccggcg ctggtaatcg ccctgatgcg cagtttcggc tggcgcaagg
     3661 cgtttttggc tttcgccgct ttcggaatcg tctggggcgt tatctggttt tggtggtacc
     3721 gcaacgagcc cgaaaagcac cggtcggtgc gcgcggaaga gttgcgggag atccgcggcg
     3781 atgcggggat tccaacggcc gcttcgctgc cgtggaagca aatgtggcgc agccggaatc
     3841 tttgggctct gtgtgcgatg tattccggat acacatacgg cctctacttc tacctgactt
     3901 ggcttccaac atatcttcgc cagcagcgcg gcatgtcgtg ggcgggcgtc ggcgtcgggg
     3961 ccgctttgcc gctgctcatg ggggcggcgg ccaacacggc cggcggatat tggaccgact
     4021 ctctcgtaag gcgcttcggc ctgcgctggg ggcgccggat tccggccatc tcagggctgc
     4081 tggcctcggc cgtcctgttg gccgcggccg ccttgacgcc cgcgaatgcg cccgcggtcg
     4141 ctgctctagc cgctagcttc ggcgccgcgg atttgattgt tgccgtctgc tgggccacct
     4201 gcatcgacct aggcaagcag aatgccgggg ccattgccgg cgtgatgaat tccctcggcc
     4261 agatcggggg tatgatttca cccttcctgt tgggctggct tttcgaaaaa tcagggtcct
     4321 ggaccctgcc tcttctgata tcggcgggtt actatgccgc cagcgcagcg ctatggctcg
     4381 tcatcgatcc cgaggacgat cgatacctgg cggcctcgtg agccgcgctc cgcggagcgg
     4441 ccgccccggc gcagcgtgtt tgtggtcctg catccgcgtg ggctgcgagt gcaaggcgtg
     4501 cttcgcgagc tgcggaatta tggatccggc catccttccc gggaaatgcg gcgatggcag
     4561 ccgccctccc ggtttgctat atttgtgttt ccaccagtcg aatgtttctc gccgcgggtt
     4621 gcttcagggg tcgcgctgtg cgcggtagcc aagcctcatg accctcgccc ctattgacta
     4681 cctgattctc gccatctatt ttgccttcgt actaggcatt gggtattggc tccgcgcgaa
     4741 agtgcattcg agcgaggagt tttttctctc cggccggtcg attcccttgt ggatcaccgg
     4801 cctggccttc gtggcagcca acctaggggc gcaggaggtc atcggcatgt gcgcctccgg
     4861 cgccaagtat ggcatcatga ctgcccattt ctactggctc ggagccgttc cggccatggt
     4921 tttcgtgggt gttttcatga tgccgtttta ctacgg
//
LOCUS       DASREK010000225         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_779653, whole genome shotgun sequence.
ACCESSION   DASREK010000225 DASREK010000000
VERSION     DASREK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779653"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFA54_11765"
     CDS             <1..562
                     /locus_tag="VFA54_11765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZS51533.1"
                     /translation="SRPVVEFPIPACEPEGALPEGDFVLASPIAGWTAKQWPASYYAH
                     LAGLLQRRFGWPLVINCAPGQQEIAGEIMSQAPRGSCVQNVGSVAGLIGATRLARAVI
                     GSDSGPTHLAALMGKPGVALYGPTDPARNGPYGSGFAVLRARNAQTTHERAKVISASM
                     RALDPERVFQALETQLAQHSVLESQR"
     gene            559..1629
                     /locus_tag="VFA54_11770"
     CDS             559..1629
                     /locus_tag="VFA54_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZS51534.1"
                     /translation="MKRLVLLFVAAAALPAQQTGPLLDRPSAIQAYQQAVNLMDAASV
                     AIPALAAAGAQFVANARQDAAALETSALYNNSAVLYRLLTNLRIYLDLSDALPKPNPF
                     AEDARKQLASLRDIHDRLEAHFRAVLESKEQQLRSPDRENVSRYAEANRSLGPPRPNE
                     NRVVFLGDSITDAWRLNEYFPDKPYINRGIGGQITGQMLGRMKADVIDLKSVAVLILG
                     GTNDIARGIPAAAIENNFSMIADLAVANHIRPIFASLLPVSDYHKDQNPDYERTKLRP
                     PAVIREINAWLEAFCKQHGFTYCDYFTAAADSQGFFRSDFSDDGLHPNAAGYRAMGLI
                     AQSAINRSLAPPPKRKGKRSAG"
     gene            1737..2387
                     /locus_tag="VFA54_11775"
     CDS             1737..2387
                     /locus_tag="VFA54_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HZS51535.1"
                     /translation="MTIFIAGGTGYMGQSLIPRLLAQGHCVRALVRPGSAMKLPAGCQ
                     PVTGDALDSASFQDQVSGADAWVHLIGVPRPNPSKAAEFRTVDLASVQAAAQAAQAGG
                     IAHFVYVSVAQPAPVMKAYVAVRAECENAIRAAGLNATFVRPWYVLGPGHWWPLALLP
                     FYKLAEIVPFTRETAQRLGLVTRSQMTAALAWAVNHPCQGIRILSVREIRTCPENM"
     gene            2482..>3427
                     /locus_tag="VFA54_11780"
     CDS             2482..>3427
                     /locus_tag="VFA54_11780"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HZS51536.1"
                     /translation="MWKCRVPPAARRLLPIVLCMLTPAVPGAAAESALSSLPGAPPFD
                     SQLAEKIQAAWAAKPASYRPRTRHLRPDGSPLYTNRLFLETSPYLLQHAHNPINWYPW
                     GAEAFETAKKLGRPVFLSIGYSTCHWCHVMEEESFEDEVIARYLNENYVAVKVDREEH
                     PDVDAVYLSALQNLTGSGGWPMTLWLTPERQPYFAGTYFPPRHRPGVSGLLSLLRSLK
                     EVYVNEPASVTGQAARITYGIQQSFTLKSGVGQPQTSIIYRAANFYAAEFDAQYGGIS
                     ESPKFPSSLPLRFLLRYQRRTGDEQALRMVTLTLEKMAA"
BASE COUNT          641 a         1103 c         1014 g          669 t
ORIGIN      
        1 ttcccgcccg gtggttgagt tccccatccc ggcgtgtgag ccggagggcg ccttgccgga
       61 aggggacttc gttctggcaa gcccgattgc cggttggacg gccaaacagt ggcctgcctc
      121 ctattacgcg cacctcgccg gtttgttgca gcggcgtttc ggatggccgc tggtgatcaa
      181 ttgcgccccc ggccagcaag agattgcggg agaaatcatg agccaggcgc cgcgtggttc
      241 ctgtgttcag aacgtcggct cggtggccgg tctgattgga gccacgcgtc tggcgcgcgc
      301 ggtcatcggc tcggatagcg gccccacgca tttggccgca ctcatgggca agccgggcgt
      361 cgcgctgtat ggccccacag atcccgcccg caacggcccc tacggttccg gcttcgccgt
      421 cctgcgggcg cgaaacgcac agacgaccca tgaacgcgcc aaggtgatct ccgccagcat
      481 gcgcgcgctc gatccggaac gcgtattcca ggcacttgaa acacagctcg cacagcattc
      541 cgtgttggag tcacagcgat gaagcgattg gttctcctct ttgtggccgc cgcggcgctt
      601 ccggctcagc aaacaggccc cctgctcgac cggccgagcg ccatccaggc gtatcagcaa
      661 gccgtaaatc tgatggatgc cgcctccgtg gccattccgg cgctcgcggc tgcgggagcg
      721 cagtttgtgg caaatgccag gcaagatgct gccgcgctcg aaaccagcgc cctctacaac
      781 aacagcgccg tgctctatcg gctgcttacc aatctccgca tctatctgga cctgtccgac
      841 gcgcttccca aaccgaatcc gttcgccgaa gacgcccgga agcagttggc ttccttgcgg
      901 gacatccacg accgtctgga ggctcatttc cgggcggtcc tcgagagcaa agaacagcag
      961 ttgcgcagcc cggatcgcga gaatgtaagc cgctacgccg aggccaaccg ttcgcttggc
     1021 ccgccccgcc cgaacgaaaa ccgtgtggtg tttttgggcg actctatcac cgatgcctgg
     1081 cgtctgaacg aatatttccc ggataagccc tacatcaacc ggggcatcgg agggcagatc
     1141 acagggcaaa tgctcgggcg gatgaaggcc gatgtcatcg acctgaaatc cgttgcggtg
     1201 ctgatcctgg gcggcacgaa tgacatcgcg cgcgggatac cggcggcggc gattgaaaac
     1261 aatttttcca tgatcgccga cctggccgtc gccaatcaca tccgcccgat ctttgcctct
     1321 ctgttgccgg taagcgacta tcacaaagac caaaatcctg actatgaaag gaccaaattg
     1381 cgcccgccgg ccgtcattcg cgagatcaac gcctggctag aggcgttctg caaacagcac
     1441 ggcttcacct attgcgacta ctttacggcg gccgcagact cacaaggctt tttccggtct
     1501 gacttttccg atgacggcct gcatcctaat gctgccggct accgcgccat gggtctcatc
     1561 gcacaatcgg cgatcaaccg gtccctggcg cctccgccga aacgcaaagg gaagagatcg
     1621 gcggggtaaa tcagccgccg ggttcacaga gcgtgggctc gccggatgtc gtccgccgaa
     1681 cggctccttc acggcgggct gtagcctcag gcgtcgccca cagacgacca agcgctatga
     1741 cgattttcat tgcaggcggc acgggctata tgggacagtc gctgattccg agacttttgg
     1801 cgcaaggcca ttgtgtgaga gcgctggttc ggccaggctc ggcgatgaaa ctgccggcag
     1861 gctgccagcc cgtaaccggc gacgcgcttg acagcgcctc ttttcaagat caggtatccg
     1921 gcgccgatgc ttgggtccat cttattggcg tccctcggcc caacccgtca aaggcagccg
     1981 aattccgcac ggtcgacttg gcttctgttc aggcggcggc gcaagcggct caggccggcg
     2041 gcattgcgca tttcgtgtac gtgagcgttg cccagccggc tccggtcatg aaggcatatg
     2101 tcgctgtgcg ggccgaatgc gagaacgcga ttcgagcagc cggcctgaac gcaacatttg
     2161 ttcgcccctg gtacgtgttg ggccctgggc attggtggcc cttggcgctc ttgcctttct
     2221 ataagctcgc tgaaatcgtt cccttcaccc gggaaaccgc gcagcgcctg ggattggtaa
     2281 cccggagcca gatgactgcc gcgctggctt gggccgtgaa ccacccgtgc cagggaattc
     2341 ggatcctttc ggtacgcgag attcgaacct gcccggaaaa tatgtagcgc aggcttcgag
     2401 ccgcgatccc gttttttcca gacgttcgcg gtcattccca ttcgggcttg gtataaaaga
     2461 gtgagatcca ggagccggaa tatgtggaaa tgcagagtcc cgcccgccgc tcgccgcctg
     2521 ttgcccattg tactgtgcat gctgacgccg gccgttcctg gggcggcggc cgaaagtgcg
     2581 ctgtcctcac tgcccggagc gccgccgttc gactcgcaac ttgcagagaa aatacaagct
     2641 gcgtgggccg ccaagccggc ctcttaccgc ccccgcacgc gccacttgcg tcccgacggc
     2701 tcgccgctgt acacgaatcg cctttttctc gaaaccagcc cctatctgct gcaacatgcg
     2761 cacaacccaa tcaattggta tccgtggggc gcagaggcgt ttgaaacagc aaagaagctg
     2821 gggcgtccgg tatttctgag catcgggtat tccacctgcc actggtgcca cgtgatggag
     2881 gaggagtcat ttgaagacga agtcattgcc cgctatctca atgagaatta cgtcgcggtg
     2941 aaagtggatc gcgaggagca cccggatgtt gacgcggtgt acttgagcgc cctccaaaac
     3001 ctgaccggca gcggcggctg gcccatgacc ctctggctca cccccgagcg ccagccctat
     3061 ttcgccggca cctattttcc accccgccat cgtcccggcg tcagcggact gctttccctg
     3121 ctgcggtcgc tgaaagaggt ctacgtgaac gaaccggcga gcgtgaccgg gcaggccgcg
     3181 cgcatcactt atgggatcca acagtcgttc acactgaaat ccggcgtggg ccagccgcaa
     3241 accagcatta tctatcgcgc ggcgaacttc tatgcggccg aatttgacgc tcagtacggc
     3301 ggaatttcgg aatctcccaa atttcccagc agtcttcccc tccgcttcct gctccgctac
     3361 cagcgccgca ccggagatga gcaggcgctc cggatggtga ctctcacgct cgaaaagatg
     3421 gcggctg
//
LOCUS       DASREK010000226        12876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_779817, whole genome shotgun sequence.
ACCESSION   DASREK010000226 DASREK010000000
VERSION     DASREK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779817"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFA54_11785"
     CDS             complement(<1..810)
                     /locus_tag="VFA54_11785"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZS51537.1"
                     /translation="MSRSLLYKAIFILVVVLGCIYGVIEVPRSKAELLGNLQKRIHLG
                     LDLKGGSHLILQVQVQDAVRATADQVIEQLRTDLNRQNITYTSIDRNDPQTIEQADSI
                     EIRVRGIPSERSSDFKALIEDRFPEWLYTPIDSTSWRLNMKPAALSQLKQDTVQQSLR
                     TIDSRINGLGLTEPTIQPYGGPDEYRILVQLPGVDDPARVKEIMQTTALLELKQVLGG
                     PYPSVDAALASHGGVLPENSEVLRSVERGDAGQQSPENWYIVSRTPVITGRD"
     gene            complement(823..1143)
                     /gene="yajC"
                     /locus_tag="VFA54_11790"
     CDS             complement(823..1143)
                     /gene="yajC"
                     /locus_tag="VFA54_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZS51538.1"
                     /translation="MIVSAIAALLQAPASAPGLGQFLPFILIFVVFYLVLFLPMQRKQ
                     KKQKQMLEALKNGDQVLTSGGIVGTIVGLNDDNTIVLRVKPDNVKLLFARSAVSTIVG
                     EEKK"
     gene            complement(1200..2336)
                     /gene="tgt"
                     /locus_tag="VFA54_11795"
     CDS             complement(1200..2336)
                     /gene="tgt"
                     /locus_tag="VFA54_11795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZS51539.1"
                     /translation="MTERAVRFEVVRECPHTRARAGLLHTPHGIVETPVFMPVGTLGA
                     VKALTQDMLEELGARIILANTYHLLIRPGHELVAEMGGLHRFLSWPNAVLTDSGGYQI
                     FSLSSLRQINDDGVIFRSHLDGAEHHLTPESALDIQAALGSDIRMVLDECTEYPTTHE
                     HARRSMERTVRWARQGLAHARRQGRERPPAVFPIVQGSMFPDLRRECAERLLELDADG
                     YAIGGLSVGEPRTVSLEMVEATEEILPRDRPRYCMGVGLLEDLGEYVARGVDMMDCVL
                     PTRNARNGYLFTSRGRLIIKHQKYRNDPRPVDEDCPCRTCARYSRAYLRHLFLSGEIT
                     FSVLASYHNVRAYLDRLRRIRQSIMLGNLPTLLNSFSYQTVDEQ"
     gene            complement(2339..3073)
                     /locus_tag="VFA54_11800"
     CDS             complement(2339..3073)
                     /locus_tag="VFA54_11800"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HZS51540.1"
                     /translation="MSDFGPKGKKLAVAAAAERRMPLIDVFFSPVETMDFLASNPRFL
                     SALLLTTVITILATTVAFERGVIERSLDQKIQADSRLQQLPEEEREAAIDRASRLNAY
                     AAIAGAMFGPAAGLLSASGVLLVLVRAVGDTRTRFAQMAGIVAHSWLPATLSTLIAIP
                     VLLLKDPESVDFDNIVPLSNLSFLFSPQEQRRLHDIGASLDLFSFWVIALLTLGVSRL
                     SGKSKTTALVIVLAPWMLYIVVFRAL"
     gene            3194..3673
                     /locus_tag="VFA54_11805"
     CDS             3194..3673
                     /locus_tag="VFA54_11805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS51541.1"
                     /translation="MRFYKKMRTTAVKCIGQCELDSPNMHLKFHQVIESRLDQIDEIV
                     QRVMTRVRRMPGARRHLEEVTLALAEAVANAVIHGNRSDPGKKITICGGCENGRTLVL
                     VITDEGEGFDPSGLPDPTVSENIFATHGRGVFLIKQLMDQAEYRKGGRQLVLRKRLS"
     gene            3802..4734
                     /locus_tag="VFA54_11810"
     CDS             3802..4734
                     /locus_tag="VFA54_11810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZS51542.1"
                     /translation="MSDSYVPMRRHSERKARSGLAVFFAVLVAGSAYFEHKILVLGGS
                     IQNHLEIIFALVWWVTVASIVARLVMRESPRDVSFRWGGSVGTRAIAVATAMPLVVGF
                     TAYGIGWGTGLADFRAPGLSRVVLGMHFTFAANPIVRFSESLLILLVLGALDNFKYAA
                     GEEIGWRGYMLTRLADSGVRAPILISGLAWGLWHAPLILSGQYASGRYPLLSVCLFLV
                     GIVAAAYVFAWLRLSSGSIWPCIWAHGAWNAVIQGVFDRSTLGPSVWVGESGVLTSVM
                     LILFAVVLYKVCPLARNTSLSTEPNWAAAVSGKT"
     gene            complement(4912..5400)
                     /locus_tag="VFA54_11815"
     CDS             complement(4912..5400)
                     /locus_tag="VFA54_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZS51543.1"
                     /translation="MTVVPNGEPYTSLDVARVSGVSLRQLQWWDEQGVVSPSHSGHRR
                     LYRREEVIAVAVIAELRRKGFSLQKIRRVLKFLEREMGKGLFESVKNGAEVHLLTDGR
                     NIYLENDYRRIVNILKNARQPMISVCVSDQVKRLMPPAAPLPKKVSRETAAARRPARA
                     AL"
     gene            complement(5524..5745)
                     /gene="thiS"
                     /locus_tag="VFA54_11820"
     CDS             complement(5524..5745)
                     /gene="thiS"
                     /locus_tag="VFA54_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZS51544.1"
                     /translation="MASTTQEIEIQLNGEKFNVTLGSTVADLIRRLDLAEDRVAVEVD
                     RRIVRRPYWNEERLQPGAAVEIVQIVGGG"
     gene            complement(6143..7129)
                     /locus_tag="VFA54_11825"
     CDS             complement(6143..7129)
                     /locus_tag="VFA54_11825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZS51545.1"
                     /translation="MQYRSLGKGGPRVSTIGLGCMGMSEFYGPVDDKESIATIHRALD
                     LGVNFLDTADVYGLGRNEELVGRAIRERRKDVFLATKFAIVRSSEGALLGVSGKPAYV
                     RSACEASLRRLGVETIDLYYQHRVDPDVPIEETVGAMADLVRQGKVRYLGLSEAGPRT
                     IRRAHAEHAITALQTEYSLWSRDPEDEVLPAVRELGIGFVAYSPLGRGFLTGRFKKFE
                     DLPPDDYRRNSPRFQGENFQKNLDLVRRIEVIAKEKGCTPSQLALAWVLAQGDDIVPI
                     PGTKRRSYLEENIAALEVHLSAGELARIDEVAPRGAASGARYPEHMMRLVGR"
     gene            7231..8136
                     /locus_tag="VFA54_11830"
     CDS             7231..8136
                     /locus_tag="VFA54_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZS51546.1"
                     /translation="MSLVVVGSVAYDGIETPYGKADRLLGGSATYIALAASYFTAVKI
                     VAVVGEDFDGKDWRLLEDHGIDLQGLERVPGRTFYWAGSYSPDLNDRTTITTELNVFA
                     GFDPKLPEEYRSAPYLFLGNIQPSLQDRVRRQMNHPRLVGGDTMNLWIREAMRDLAET
                     LRGWDVLLVNDSEARMLTGESNLRAAAQKILNMGPHTVIIKRGEYGAVLFQRDGYFIT
                     PGFLLESVYDPTGAGDSFAGGFMGYLAGGGAHPATHAALRRAVIYGSVMGSFCCEEFG
                     VRRLARLTRAEIDARYREFQAFTHF"
     gene            8168..9688
                     /locus_tag="VFA54_11835"
     CDS             8168..9688
                     /locus_tag="VFA54_11835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS51547.1"
                     /translation="MKRSNPSAAREILLCLLCAAAVSAAFVWFCLARGYTLYYGDAAA
                     HINVARRIIDSRRPGYEQLGTVWLPLPHVLMLPLVRSDSMWRSGAAGAIPAALCFVVG
                     AVCFYAAVRRATGSRAGAITAAAAFGLNPNLLYLQSTPMTEAVFFGCFAAAVLFCAAF
                     QARPSAWRALGAGAAVLAASLTRYEGWFVIPFFALFFWISGSGKHAAIFAALASLGPL
                     YWLGHNAYIYSDPLEFYRGQFSAHAIYARALAGGLPRYPGDHDFHAAWKYYKEAMELV
                     MGRPLVWMAPAGAAIALIKKSARPLLLLALPIPFYILSLHSGGTPIFVPKLYPYSYYN
                     TRYALAALPACAAAAGLIAAVWPASWLRAAAAAALLTVCLGGWVLHPGVNSWICWKES
                     QVNSEGRRLWTRQAAEFFRSHYRPGDGVLTAAGDVTAVYQQAGLRLCDTLSDGPAFDW
                     AVARPDLFLHERWVAAVAGDTISFRVNSPQFARWFEPAAQFSAQREPVIEIYRKIK"
     gene            9685..10536
                     /gene="dapF"
                     /locus_tag="VFA54_11840"
     CDS             9685..10536
                     /gene="dapF"
                     /locus_tag="VFA54_11840"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZS51548.1"
                     /translation="MKVQFAKAHGDGNDFLLVRGLAWAEDADYAALARAICDRHRGVG
                     ADGLVVFTGPARIRLFNSDGSEAELSGNGTRCAAALLAADGEAHSAGASPEESPGQAG
                     DTFDIETTAGRKHLRLLARDGYRFQFEMNMGRPCYSPEEIGCPLETALGTWKATLVNV
                     GNPQCAVLVSDFDFAWRELGRAIEEHPRFPNRTNVSFIKVRDSHTIEVRFYERGAGPT
                     LSSGTGATGAAIAAILTGRVQSPVRVVTEAGSLELDWNGGDVLLRGPAEITARGEFYW
                     RRGKETP"
     gene            10533..12350
                     /locus_tag="VFA54_11845"
     CDS             10533..12350
                     /locus_tag="VFA54_11845"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZS51549.1"
                     /translation="MTGSLEALDRPVIVWGVKRLLILLCCATAALAETHYEIDVARAP
                     LHVLLVSIETDCPKIDCDFQMPVWNALYQIRDFAQFVNRFEAAGTAGKPVVYRMLTPS
                     LWRVKAAPGEHVRIRYEYFADSPGPFGCSASARHVFLNFAQILMYPVGRLREPASVAF
                     RNVPAAWKIAVELPERKGSFRARNYDELVDAPVEMSAFQEADFTLAGKRMRVVVDGDP
                     EDYNLNLLEDTARRVAATAAEIMQDVPFPSYTFIYHFRKGGRGGMEHANSCAIDAAAP
                     CRNCTLAGVTAHEFFHLWNVKRIRPQSLEPIDYTRENITPSLWFSEGVTSTMGAYIQL
                     QAGLLEPRDFLSRLESGITSYEQLPARLTQSAEEASIEAWLERYPFYERPGRSVSYYL
                     KGELAGYLLDLTIRHCSAGRKSLDDLMRRLNMAYAKKGSFFDDTDAIQRAASEVAGRD
                     LKDFFDAVVRSAAPIDWDKYLQYAGYRLAPTVQYVPTLGIETERIAGPAWVLSRLEAG
                     GAAEQAGLEPGDRLVALDQRPIRRDPDLELSSLALKPGAGVSLEIERGSRKMSVEVYP
                     AFVRRVTYKLEEITAPTVEQRLIRAGWLRGRVEQDERRR"
     gene            12473..>12876
                     /locus_tag="VFA54_11850"
     CDS             12473..>12876
                     /locus_tag="VFA54_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51550.1"
                     /translation="MRREEAEGSRLVAAVARARRRVITNQGFREAGWALTIALLGPTA
                     FLALGKDWFAWPLLVIFALAGTGFAGWRLWKRRPGAYEISQALDARLATADQISTAIY
                     FLDSRDPVAVEQRRRAATLTERIDLDAAFPFTV"
BASE COUNT         2432 a         3865 c         3936 g         2643 t
ORIGIN      
        1 atcccgcccg gtgatcaccg gcgtgcggct tacgatgtac cagttctccg gagactgctg
       61 gccggcgtct ccccgctcga cggaacgcaa cacttcgctg ttttccggca gcacgccgcc
      121 gtgcgaagcc agcgccgcgt cgactgaagg ataaggcccg cccagcacct gcttcagctc
      181 cagcagggct gtggtctgca tgatttcctt cacccgagcc gggtcatcca cgccgggcaa
      241 ttgcaccaga atccggtatt catccggccc gccatagggc tggatcgtcg gctcggtcag
      301 acccaggccg ttgatgcggc tgtcgatggt gcgcagcgac tgctgcaccg tatcctgctt
      361 gagctggctc agcgccgctg gcttcatgtt caggcgccag gaagtcgagt cgatcggcgt
      421 atagagccat tccgggaaac ggtcttcaat cagcgccttg aagtcgctcg accgctcgga
      481 cgggatcccg cggacgcgga tttctatact atcggcctgc tcgatggtct gcggatcatt
      541 gcggtcgatg gaagtgtaag tgatgttctg ccggttcagg tcggtccgga gttgctcgat
      601 cacctgatcc gcggtggcgc ggacggcgtc ctgcacctgc acctgtaaga tcagatgcga
      661 cccgcccttc aaatcaagac cgaggtggat gcgcttttgt aagttgccca gcagttcggc
      721 cttcgaacgc ggcacctcga tgacgccgta aatacagccg agcacaacca ccaggatgaa
      781 aatggcttta tagagaaggc ttcgcgacat ggtttataaa tactactttt tctcctcgcc
      841 gacgatcgta gaaaccgcgc tccgggcgaa cagcagcttc acgttgtcgg gtttgacccg
      901 cagaacgatg gtgttatcgt cgttgaggcc gacgatcgtg ccgacgatgc cgccgctggt
      961 gagcacctga tccccgttct taagagcttc cagcatctgt ttctgctttt tttgctttct
     1021 ttgcatgggg agaaacagca ccaggtagaa aacaacaaag attagaatga aaggcaggaa
     1081 ttgtccaagg ccaggcgccg aagccggcgc ctgcaacaga gctgcgatgg cagacacaat
     1141 catgattcca aattctgttc cgcgcgcgtt ttttaagaca taccgcgacc gggatgccgc
     1201 tactgctcgt ccaccgtctg ataactaaat gagttcagga gagtcggcag gtttccaagc
     1261 attatagact gcctgattcg ccgcagcctg tcaaggtacg ctcgtacatt atgataactc
     1321 gccaggaccg aaaaagtgat ttccccgctg aggaacaaat ggcggaggta tgcgcgcgag
     1381 taccgggcgc aggtacggca tgggcaatcc tcatcgacag gccgggggtc gttccggtac
     1441 ttctggtgct taataatgag gcggccccgg ctggtgaaca agtagccgtt tcgagcgttg
     1501 cgcgtcggca ggacgcaatc catcatgtct acgccccgcg ccacgtattc gcctaaatcc
     1561 tccaacaagc ccacccccat gcagtagcgg gggcggtccc gaggaagaat ttcctctgtc
     1621 gcttccacca tttccaggct gacggtgcgc ggttcaccca ccgaaagccc cccgatggcg
     1681 tatccgtcgg catccagttc caacagccgt tcggcgcact cgcggcgaag atccgggaac
     1741 atcgaccctt gtacgatcgg aaagacggcc ggcggacgct cccgtccctg gcggcgggca
     1801 tgggccagtc cttgccgtgc ccaccgtacc gtacgttcca tggaccggcg ggcgtgctcg
     1861 tgcgtggtag ggtattcggt gcactcatcc aaaaccatgc ggatgtcgct tcccagggcc
     1921 gcttgaatat caagcgcgga ttcgggcgtg agatggtgtt cggccccgtc gaggtgagag
     1981 cggaagatca ccccgtcgtc gttgatttgc cgcagcgagc tgaggctgaa gatctggtag
     2041 cccccggagt cggtaagcac cgcgttcggc caactcagaa agcgatgcag cccgcccatc
     2101 tcggcgacca gttcgtgccc gggccggatc aacaagtgat aggtattggc cagtatgatt
     2161 cgagccccga gttcctcgag catatcctgg gtgagcgctt ttaccgctcc cagagtcccc
     2221 accggcatga acactggcgt ttccacgatg ccgtgcggcg tgtggagcaa gccggcccgc
     2281 gctcgtgtgt gcgggcattc ccggaccact tcaaaacgca ctgcccgctc ggtcatcctt
     2341 acaaggcccg aaaaacgaca atgtagagca tccacggagc cagcacaatg acaagcgcgg
     2401 tggttttgct tttcccgctc aaccgggaga ccccgagcgt aagcagagcg ataacccaaa
     2461 aggagaacag atccaagctg gccccgatgt cgtgcagcct gcgctgctcc tgcggcgaaa
     2521 acagaaagct gagattggag agcggcacga tgttatcaaa gtcaacggat tcgggatctt
     2581 ttaaaagcaa aacgggaatc gctatcaatg tgctcaacgt agccggcagc caggaatgcg
     2641 ccacaatgcc tgccatctgc gcaaaacgag tccgcgtgtc acccaccgcc cggacgagca
     2701 ccagcaagac gccgctggcg ctgagcaacc cggccgcggg cccgaacatc gctccggcga
     2761 tggccgcata cgcattcagc cggctggccc ggtcgatagc cgcttcgcgc tcctcttcgg
     2821 ggagttgctg aaggcgggag tccgcctgga ttttctgatc gaggcttctc tcgattaccc
     2881 cacgttcgaa ggcaaccgtt gtagcgagaa tggttatgac ggtagtaagc aggagcgccg
     2941 ataaaaaacg tgggttgctc gccagaaaat ccatggtctc gacaggcgag aaaaagacgt
     3001 cgatgagcgg catccgccgc tcagccgcag ccgcaacggc caattttttt cctttagggc
     3061 cgaagtcaga catggacgcc aagtgtaatc cgccggggcc ggctgcggca agctctccgt
     3121 acgaggccaa gcgcgtcttt cgagcaaccc ggtcggcgta gggctggcag cagcgccttt
     3181 cagttaaaag agtatgcgat tctacaagaa gatgaggacc acggcggtga agtgtatagg
     3241 tcaatgcgaa ctcgactccc ctaacatgca cctgaaattt caccaggtca tcgaaagccg
     3301 cctggatcag atagatgaaa tagtacaacg cgtcatgacc cgagtccggc ggatgcccgg
     3361 tgcgcgccgg catctagagg aagtgacgct ggcgctggcg gaagccgtgg cgaatgccgt
     3421 gattcacggc aatcgaagcg accccgggaa gaaaatcacg atctgcggcg gctgcgagaa
     3481 cggccggacg ctggttctgg taattaccga tgagggggag gggttcgatc cctccgggct
     3541 tcccgatcct accgtttcgg aaaacatttt cgcaacgcac gggcggggcg tttttctcat
     3601 taagcagctc atggaccagg cggaatatcg caaaggcggc cgccagctcg tgctgcgcaa
     3661 acgcctctcc tgaattgaca taaagcccgt ttaacctccc tcagcacaat tcaatttcta
     3721 tcgcctgtga atgtcatcca aacagtaatc aagcgatttc ttgttagagg agctgccgta
     3781 gcgtaaggta ggatgatggc cttgagcgat tcctacgtcc cgatgcgcag gcattcagaa
     3841 cgtaaggcac gctccggtct ggctgttttc ttcgcggtgt tggtagccgg gtcggcctat
     3901 ttcgagcaca agatcctggt tctgggtggg tctatccaga atcacctgga gatcattttc
     3961 gcgctggtgt ggtgggtgac ggttgcatcc attgtggcgc ggctggtcat gcgtgagtcg
     4021 ccccgtgatg tttccttccg ctggggtggt tcggtgggga ctcgcgccat cgcagtcgcc
     4081 actgcaatgc cgcttgtggt cggattcacc gcctatggaa tcggctgggg cacaggcctg
     4141 gcagatttca gggcacctgg gctctcccgc gtcgtgttgg gaatgcactt tacttttgct
     4201 gcgaacccaa tcgtgcgatt ttcggagtcc cttttaatac tgttggtcct tggcgcgctc
     4261 gacaacttca agtacgcggc cggtgaagaa attggctggc gcgggtacat gttaacccgg
     4321 ctggcggatt ccggcgtccg ggcgcccatc ttgataagcg gcttggcgtg gggactgtgg
     4381 cacgcacccc ttattttgag cgggcaatat gcttcgggcc ggtatcctct cttgtcggtg
     4441 tgtctgtttc tggtcggcat cgtggctgct gcatatgtct ttgcatggct gcggttgtcg
     4501 tcaggaagca tctggccctg catctgggcg cacggggctt ggaacgcggt gattcagggc
     4561 gtattcgaca ggtctacttt gggccccagc gtgtgggtgg gtgaatccgg cgtgctcacg
     4621 tcagtcatgc tcattctctt cgcagttgtg ctctacaagg tgtgcccttt ggcgcggaac
     4681 acgtcgctat ctacagagcc gaattgggca gcggcagtca gcggaaaaac gtagccactt
     4741 gcggtcagga cgctgccggc gaatcaggaa tattcttccg ggctttggcc caacaaacat
     4801 tgtatctgcg gaagtggact cactgaattc cgattgactg gatcccgatg cgggcaggcg
     4861 gcccttacgg gcgggtaggg aaaaaagagc gctcgcggta aaaaaattat tttagagagc
     4921 tgccctcgcc ggccggcggg ccgcggccgt ttctcggcta actttcttcg gcagaggcgc
     4981 cgcagggggc atgagccgct tcacctgatc ggagacgcat accgaaatca tcggctggcg
     5041 cgcgttctta agaatgttga cgatccgccg gtaatcgttt tccaggtaga tattgcggcc
     5101 gtcggtaagc aggtgcacct ctgcaccgtt tttgaccgac tcgaacagcc ccttgcccat
     5161 ctcccgttcc aggaatttca acacgcggcg gatcttttgg agcgaaaagc ccttccgccg
     5221 aagctcggcg atgacggcga cggcgatcac ctcttcgcgg cgatagagac gccggtggcc
     5281 gctgtgactg ggagacacca cgccctgctc gtcccaccat tgcaactggc gcaggctcac
     5341 tcccgacacg cgggcgacgt cgaggctcgt gtacggctcc ccgttcggta caaccgtcat
     5401 tttcggcttt cttgttttga acattcttta cgattccgtt tccgtcagaa ttggagctcc
     5461 cgcactataa acccaacctg accgcctgtc aaccttaaat gtatttacca gaaaagaaac
     5521 gggttacccg ccgcctacga tctgcacaat ttccacagcc gcgcccggtt gcagccgctc
     5581 ttcgttccag tacggccggc gcacgatgcg ccggtcgact tccacggcca cccgatcctc
     5641 cgccagatcc aaccggcgga tgaggtcggc taccgtagac cccagcgtaa cgttaaactt
     5701 ctccccattc agttggattt cgatctcttg cgtcgtgctt gccacgttcc ttttggcctc
     5761 cattatagta aaacgtctat agtaaaacgt cgctggctgg ccgtggaagt tttcttcaag
     5821 tgttctggtt gacccacacc ttttcggaga tgccgctggt tccgcggaaa tctgcccgcc
     5881 gcccgtcaaa atttactcgc atgcaaccgc cggcagggcg gccattggcc gaaacggcac
     5941 ggtgatattt accttgctgc gcggcgcaat gatttccgga cagttgccgc caacacccgc
     6001 ccgctggctg gccagcgggg acgccgcctc cggaatcctc ttccggctcg accggaggcg
     6061 gagcgggagg gaaaagagtc ctgagacgcg cacccctaag ttcagttgcc ccgttgaaat
     6121 ttcgatggac ttgggccggc cgtcagcgcc ccaccagccg catcatgtgt tccgggtagc
     6181 gcgcgccgct tgccgctccc cggggggcga cttcgtcgat gcgcgccagt tcgccggccg
     6241 aaagatgcac ctccagagct gcgatattct cttcgagata cgagcgccgc ttcgtgccgg
     6301 gaatcggaac aatatcatcg ccctgggcca gcacccacgc cagggccaat tgcgatggcg
     6361 tgcagccctt ctctttggcg atgacctcga tgcggcggac gagatcgaga ttcttctgaa
     6421 aattttctcc ctgaaagcgc ggcgagttgc ggcgataatc atcaggagga aggtcctcaa
     6481 atttcttaaa ccggccggtc aagaaccccc gccccagcgg gctgtaagct acaaacccga
     6541 tgcccagttc gcggacagcg ggtaaaacct cgtcttcggg gtcccggctc cacagcgagt
     6601 attccgtctg taaggccgtg atggcatgct cggcgtgcgc cctgcggatg gtccgaggac
     6661 ccgcctccga gagccccaga tagcgcacct tgccctgccg cacgaggtca gccatcgctc
     6721 ccaccgtttc ttcgattggc acatccgggt cgacccgatg ctggtaatag agatcaatcg
     6781 tctcgacgcc caagcgccgc aggctggctt cgcaggccga gcgcacatac gcgggcttgc
     6841 cgctcactcc aagcagtgcg ccctcgctgc tccgcacgat ggcgaatttc gtggccagaa
     6901 agacatcttt ccggcgttcc cgaatcgccc ggcccaccag ctcttcgttg cgtccgaggc
     6961 cgtagacatc ggcggtatcc agaaaattga cgcccagatc cagcgcgcgg tggatggtgg
     7021 cgatggattc cttgtcatcg acggggccgt aaaactcgga catccccatg cagcccaggc
     7081 cgatcgtgga aacccgcggg ccgcccttcc ctaaagaacg gtattgcatt cctgctcccc
     7141 ctgcttttga aagtgattcc caattatcgc accgcccatc gcagggccgc ggcgcgggca
     7201 taacgatccc ggctgctaca atgttcatcc atgtctttgg tcgtggtcgg ttctgtcgct
     7261 tacgacggca ttgaaactcc ctacgggaag gcagaccgct tgctgggagg ttcggcaact
     7321 tacatcgcgc tggctgcgag ctatttcacc gccgtcaaga tcgtggctgt ggtcggtgaa
     7381 gatttcgacg gaaaggactg gcggctgctc gaggatcacg gaatcgatct gcaaggcctg
     7441 gagcgcgtgc cgggcaggac cttctactgg gccggctcct attctccgga tttgaacgac
     7501 cgcacgacca tcaccacgga attgaacgtt ttcgccggct tcgatcccaa actgcccgag
     7561 gaataccggt cggcgccgta tctttttctc ggcaacattc agcccagttt gcaggaccgt
     7621 gtccgccggc agatgaatca cccgcgcctg gttggcggag acacaatgaa tctgtggatc
     7681 cgcgaagcga tgcgggattt ggcggaaacc ttgcgcggct gggatgtgct gctggtcaac
     7741 gacagcgagg cccgcatgct caccggtgag agcaatctgc gcgcggctgc gcagaaaatc
     7801 ctcaatatgg ggcctcacac ggtaatcatc aaacgcggcg agtacggcgc cgtgctgttc
     7861 cagcgcgacg gatatttcat tactcccgga tttctgctgg aatccgtgta tgaccctacc
     7921 ggcgcgggcg attctttcgc cggaggtttt atgggctacc tggccggcgg cggcgcgcat
     7981 ccggccacgc atgcggcctt gcggcgggcg gtgatctatg gctcagtaat gggcagcttc
     8041 tgctgcgagg agttcggcgt ccgcaggctg gcgcgcctga cccgcgcgga aatcgatgcg
     8101 cgctaccggg aattccaggc gttcacccat ttttgacggc attgcaagcg gagggcgttg
     8161 aaggcggatg aaacgaagta atccctctgc ggcccgggag attttgctct gcctgctgtg
     8221 tgcggcggcg gtgagcgcgg cgtttgtatg gttttgcctg gcgcggggct ataccctcta
     8281 ctacggggat gccgcggcgc acatcaatgt cgcgcggcgc atcatcgact cccgccggcc
     8341 gggatacgag caactgggaa ccgtctggct gcctcttcct cacgtgctga tgctgccgct
     8401 ggtgcgctcc gactcgatgt ggcgttcggg cgcggcgggc gcgatcccgg cggcgctttg
     8461 ctttgtagtc ggcgcggtgt gcttctacgc cgccgtgcgg cgggcgacgg gcagccgggc
     8521 gggtgcgatt acggcggctg cggcattcgg gcttaatccg aacctgcttt acttgcaatc
     8581 aacgccgatg accgaggcgg tttttttcgg ttgttttgcg gccgccgttc ttttctgcgc
     8641 ggcatttcag gcccggccca gcgcgtggcg ggcgctgggg gcgggcgcgg ccgtgctggc
     8701 cgcctccctc acccgctacg aaggctggtt tgtgattccc tttttcgcat tgtttttctg
     8761 gatctcgggt tcagggaaac acgcggcaat attcgccgct ctggcctccc tggggcctct
     8821 ctactggctg ggccacaatg cctacatcta cagcgacccc ctcgagtttt accgcggcca
     8881 gttctcggcg catgctattt atgcgcgcgc gctggccggc ggcttgccac gctacccggg
     8941 agatcacgac ttccacgccg cctggaagta ctataaagaa gccatggaac tggtcatggg
     9001 ccgccctttg gtttggatgg ctccggccgg tgcggcgatt gcactgatca agaagtccgc
     9061 ccggccgctg ctgctgctgg cgctgccgat accgttttac atcctcagct tgcactccgg
     9121 cggcacgccg atcttcgttc cgaagctgta cccgtactct tactacaaca cgcgatacgc
     9181 gctggccgct ctgcccgcct gcgccgccgc ggctggactg atcgccgcgg tctggccggc
     9241 atcctggctg cgagcagcgg ccgccgccgc tttgttgacc gtgtgcctgg gcggatgggt
     9301 ccttcatccc ggcgtcaatt cgtggatctg ctggaaagag tcccaagtaa attcggaagg
     9361 acgccgcctt tggacgcgcc aggcggccga gtttttccgg agccattatc ggccggggga
     9421 cggagtcctc acggctgccg gcgatgtgac cgcggtgtac cagcaggcag gcctgcgcct
     9481 ttgcgacact ctgagcgacg gccctgcgtt cgattgggcg gttgcgcgtc ctgacctgtt
     9541 tttacatgag cgatgggtgg ccgcggtagc cggcgacacg atatcgttcc gcgtgaacag
     9601 cccgcaattc gcgcgctggt tcgagccggc ggcgcagttt tcggcgcaac gagaaccagt
     9661 gatcgagatc taccggaaaa tcaaatgaaa gtgcaattcg caaaagcgca cggcgatggg
     9721 aacgacttcc tgctggttcg cggcttagca tgggcggaag acgcggatta tgccgccctc
     9781 gcgcgtgcga tctgcgatcg ccatcggggg gtgggggcgg acgggctggt tgtattcacc
     9841 gggcccgccc gcatccgctt gttcaattcg gacggcagcg aggcggaact gtccggaaac
     9901 ggcacgcgct gcgcggccgc cctgctggcc gccgacggcg aggcgcattc ggcgggcgca
     9961 tccccggaag aatctccggg tcaggcagga gatacattcg acatagaaac aacagccggg
    10021 cgaaagcatc tgcggctgct cgcccgggac ggctatcgtt tccaattcga gatgaacatg
    10081 ggccgcccct gctattcgcc ggaagaaatc ggctgcccgc tggaaacggc acttggaacc
    10141 tggaaggcga cgctggtgaa cgtcgggaat ccgcaatgcg ccgtgctggt cagcgatttt
    10201 gactttgcgt ggcgtgagct gggccgcgcc atagaagaac atccacggtt cccgaaccgc
    10261 accaacgttt cctttatcaa ggtccgtgac agccacacga tcgaggtccg gttttacgag
    10321 cgcggcgccg ggcccacgct gagctcgggg acaggggcca cgggagctgc gatcgccgcg
    10381 atcctgacgg gccgcgtcca aagcccagtg cgggtggtta ccgaggccgg cagtctcgaa
    10441 ctcgactgga acggcggcga cgtgttgctg cgggggccgg cggaaattac ggctcgcggc
    10501 gaattttact ggcgcagggg caaagagacg ccgtgacagg ctcgctggag gcgctggacc
    10561 gccctgttat agtatgggga gtgaagcggc ttctcatcct cctctgttgc gcgacagccg
    10621 cactcgccga aacccattac gaaatcgacg tagccagagc gccgctccac gtgctgctcg
    10681 tcagcatcga aacggattgc ccgaaaattg actgcgattt ccaaatgccc gtttggaacg
    10741 ctctgtatca gatccgggac tttgcgcagt ttgtaaaccg gtttgaagcc gccgggacgg
    10801 ccggaaagcc ggtcgtctac cgcatgctca ctccctcgct gtggagagta aaagcggctc
    10861 cgggcgagca cgtccggatc cgctatgagt atttcgccga ttctcccggc cccttcggct
    10921 gctcggccag cgcccggcac gtttttctaa atttcgcgca gatcctcatg tacccggtcg
    10981 gccggctgcg cgagccggcc agcgtggcgt tccggaatgt cccggcggca tggaagattg
    11041 ccgtcgaact gccggaaaga aaaggctcat tccgcgcacg caactacgat gaactcgtgg
    11101 acgccccagt agaaatgtcg gccttccagg aggctgattt caccctcgcc gggaagcgga
    11161 tgcgcgtggt agtggacggc gatccggaag attacaacct gaacttactc gaggatacgg
    11221 cacgccgcgt cgcggcgacg gctgccgaaa tcatgcaaga tgtcccgttt ccctcctaca
    11281 cctttatcta tcacttccgg aaaggggggc gaggcggaat ggagcatgcc aactcctgcg
    11341 cgatcgacgc cgcagccccg tgccgcaatt gcactctggc gggcgtaacg gcccacgagt
    11401 tcttccatct ctggaatgtg aagcgcatac gcccgcaatc gctcgagccg atcgattaca
    11461 cgcgcgaaaa catcacgcct tctctgtggt tcagcgaagg cgtaacgtcc acgatgggag
    11521 cttacatcca gctccaggcg ggcttgctcg agccgcgcga ttttctgagc cggctggaga
    11581 gcggaatcac cagctacgaa cagcttccag cccggttgac gcaatcagcc gaagaggcat
    11641 ccattgaagc ttggctcgaa cgctatccct tttacgaacg tccgggccgg agcgtgtcct
    11701 attacttgaa aggcgaactc gcgggttact tgctggatct gaccatccgg cattgttcgg
    11761 ccggccgcaa gagtctggac gacctgatgc gccggttaaa catggcctac gccaaaaagg
    11821 gcagcttttt tgacgacacg gacgccatcc agcgggcggc gagtgaggtg gcgggccggg
    11881 acctgaagga tttttttgat gccgtcgtcc ggagcgccgc tccgattgac tgggataaat
    11941 atttgcagta cgcaggctac cgtctggcgc caacggtcca gtatgtaccc actctgggca
    12001 tcgaaacgga gcggattgca gggccggctt gggtgctctc gaggctggaa gccggcgggg
    12061 ctgccgaaca ggcgggactc gaacccggcg accggctcgt ggcgttggat cagcggccga
    12121 tccggcgcga tcctgacttg gaactgtcct ctttggccct caaaccgggc gctggagtta
    12181 gtttggagat agaacggggc tcgagaaaaa tgagcgtaga agtatacccg gcgtttgtcc
    12241 ggcgcgtcac ctacaagctg gaggagataa cggcgccgac ggttgagcaa agactcatcc
    12301 gggctggctg gctgcgcggg cgggtggagc aagatgagag acgccgataa actttttgca
    12361 actttgcgcg cccgcggata tatataatca atagattttt cagcgctcaa agccattttc
    12421 gaacggcctt ccagttgcac gtgccgttat aatggcaaag gggaagtgcg gcgtgagacg
    12481 agaggaagcg gaagggagcc ggctggttgc cgccgttgct agggcgcgac ggcgggtgat
    12541 aaccaaccag ggcttccggg aggccggttg ggcgctcacg atagctctcc tgggacctac
    12601 agctttctta gccttaggaa aagactggtt cgcttggccg ctgctcgtaa tttttgcctt
    12661 ggcagggacc ggttttgccg gctggcggct ttggaagagg cggcctgggg cgtacgaaat
    12721 ctcccaagcg ctcgacgccc ggctggccac cgccgatcag atttcgaccg ctatctattt
    12781 cctggattca cgggatccgg tcgccgtcga gcagcgccgc cgcgccgcca ccctgacgga
    12841 acggatcgat ctggacgccg ctttcccttt cactgt
//
LOCUS       DASREK010000227        12524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1589923, whole genome shotgun sequence.
ACCESSION   DASREK010000227 DASREK010000000
VERSION     DASREK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12524
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589923"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFA54_11855"
     CDS             <1..543
                     /locus_tag="VFA54_11855"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZS51551.1"
                     /translation="DVGDPRHMRLTDGRLFDYPWIYATQTAWWDLDGSETSRLREYLL
                     RGGFLVVDDFWGPEQWRLFRETMDRVLPDQPITDIAESDPVMHVLYDIQEKDRTFIPG
                     SRHLWRGPGGTVQIVQPPGTIPAWRAMYDSKNHMIVAINFNTDVADAWEFADVPYYPE
                     AMTALAYRYGINYIIYSMTH"
     gene            complement(646..1662)
                     /locus_tag="VFA54_11860"
     CDS             complement(646..1662)
                     /locus_tag="VFA54_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51552.1"
                     /translation="MRTVVLFLVLAVLTAVFAQSPSYRVTHTYMLGGEGGWDYIVPDP
                     PNHRLFIARQNRLMVVDEDNGRLLGEVTGINGAHGTALVPAAGHGFATSGNDQSVVMF
                     DLKTFKPLGRIPAAEDADAIIYDNASNRVFTLNGDAHSSTVIDPVAGKLITNIPLGGK
                     PEYGAPAGDGEVYANLTDISEVVEIDAKAATVSRRWPTAPCKQPVSMAIDTVHHRLFS
                     GCRSGVMAVSDYQTGKVVATVPIGTGVDGAGYDAASGDVFASNANGALTVIHQDAPDQ
                     YHVVENVPTPQGSRNMGLDPINHRVFLVSAKFGEAPAGGRGRRTVLPGTFTLMVIERA
                     PAKH"
     gene            2129..3676
                     /locus_tag="VFA54_11865"
     CDS             2129..3676
                     /locus_tag="VFA54_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZS51553.1"
                     /translation="MRNSFFSFFVLLASAGALFAQKVDVQELHLDNGMTFLMAPRKGD
                     PNVAAGWVARVGSVNERPGITGISHLFEHMMFKGTRTIGTTNIEEDLKILNEMDRVHE
                     QIRQEEQALVERYRRGEIDDPYNPKYRTPRHSQLIEQFEALTKRERQYTIKNEFDRIY
                     RNAGGSGMNAGTDYDYTVYFINVPSNKLELWFWMESDRLANPVFRDFYTEREVVHEER
                     RLRTDSTPTGRFQEQFDSLFWESSPYAWPVVGWPSDLESFTKEDALAYFQTYYAPNNL
                     TACLVGDFDPPKAAELARAYFGRLKRGTRAPEPVRTLEMKQLAEQRMIAYAETTPQVR
                     IRYHTVPDNHADEPALIMLAHLLSGRTGRLYKSLVLEQQIATNASASSNGLKYEGYFE
                     LTGVARQGKDPLEVEKGIYKQIENLQLTPPPERELQKVKNQQAAADFRRLQSNFGLML
                     QLLLHEAQGSWRTINTYPEKLQAVTAADIQRVAKKYFSPENRSVAIYYTKTPARTTEA
                     RNTGGGQ"
     gene            3685..5196
                     /locus_tag="VFA54_11870"
     CDS             3685..5196
                     /locus_tag="VFA54_11870"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZS51554.1"
                     /translation="MKMLAMLCGMGLVWPARVGQMAAAEIPAHPKLLQVEPLRYDPPK
                     AAQYRHVLSNGAVAYLVEDHMLPLVNLSVLIRTGRYLDPPRKTGLAAMVGAEIRSGGT
                     KNRSAQDFDEEADFLAASINSTVADTEGRATLNCLSRNFDAALELFFDMLRNPRFDQQ
                     RLALSKIRTLQTLARRNDRSNEVESREFARLLRGPSHFSTAQETKATIEAITREDLLT
                     FHQKYYHPSNFIFAVSGDFNTKDLLAKLEDLMRGWEGPKIEAPPVPKPDYAPHPGLYL
                     IDKPEVNQCRVSVGEIGTMRDNPDHYALEIMNQILGGGAFTSRIGSRVRVEEGLAYSA
                     GTMMTFGVYYEGLFRGFFQSKSASCAEAATIVLEEIQRIRAEQVSPDELATAVNYAVE
                     VFPRYFATPAAVASTFANDEYTHRPANYWETYRERLRRVTAEDVLRVARTYLHPDRLV
                     ILAVGNVSDIFKGDPDHPQYSLQKLAGGKPIVRLPLPDPVTMVYAGDPLEKAK"
     gene            5370..6344
                     /locus_tag="VFA54_11875"
     CDS             5370..6344
                     /locus_tag="VFA54_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS51555.1"
                     /translation="MLRRWLRWSLRGLMAAVTGAILFILYLTYGRPKMAAPSAVRAHA
                     TPERIARGEHLVETICDCMGCHAPRDFTRFGGPVIPETKGEGGVMPMAGLPGTIVAAN
                     ITPDPETGIGAWTDGEIMRAIREGVDKNGRALFPLMPYTDYRYMSDEDVESIVAYLRT
                     LRPIKKRQPETKINFPANLLIKLAPAPAGHVAAADRSDKHAYGKYLATIGGCLECHTP
                     LQKGGRDTTKLLAGGRLFQFPGASVRSANITPENDTGIGVFTEEFFIHKIRSYAPYAE
                     KESPKTGPESFTLMPWLNFWRMTDEELSAIYAYLRTVPPVYNAVPIHD"
     gene            complement(6347..7315)
                     /locus_tag="VFA54_11880"
     CDS             complement(6347..7315)
                     /locus_tag="VFA54_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HZS51556.1"
                     /translation="MKAIRVHQTGGPEVLQYEEIPMPTPGPGEALVKIEAAGVNFIDI
                     YFRQGLYKAPLPLTLGLEAAGVVEAVGPEATHVEPGDRVAYAGIPGAYSQYAVAPAAR
                     LVKLPEGVDARSGAAAMLQGMTAHYLATSTFPLRPGHTALVHAAAGGVGLLLIQIAKM
                     RGARVLGTVSTEEKARLAREAGADEVIIYTAQDFEAEAKRFTGGAGLDVVYDSVGRST
                     WEKSMKSLRPRGMLVLYGNASGPVPPVDPLLLSQRGSLFLTRPRLGDYTASREELLDR
                     AGDVLGWIAAGKLRLRIEHVFPLEQAAKAQEELAGRRTTGKILLIP"
     gene            7518..7808
                     /locus_tag="VFA54_11885"
     CDS             7518..7808
                     /locus_tag="VFA54_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51557.1"
                     /translation="MPETVVEADFVNLRGVHRLAGAILVQAIEDIRCGSGRRREDAIR
                     WVTDSNEDQFSFVFCCRILGRDPEEVRRFLVRQRIPDWLFSARLAEVDTAST"
     gene            8077..9261
                     /locus_tag="VFA54_11890"
     CDS             8077..9261
                     /locus_tag="VFA54_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZS51558.1"
                     /translation="MRTLVIAGLLLSLGASLLWAQQGSEAAGRAPGARSRQAGLPVPV
                     TSPEVHPDRRVTFRLFAPGAAEVKLTGEFMEGERALQKDEKGLWTITVGPLDPEIYNY
                     NFTIDGVRTIDPGNAHVKTGSTPSTIQSILHVHGDGPAFFDGQPVPHGEIHTHWYQSK
                     SLNSLRRLTVYTPPGYESNGRTRYPALYLFHGANADETAWTRLGRVNLILDNLLAAGK
                     ARPFVVVMPFGYGVPPGSTRPSLTAPGLGLSENGRLFAKDLIEDVIPYIQAQYRVYTD
                     RDHRAIAGLSMGGGQSLSIGLSRLDLFSQVSGFSAALRQADFDKTFGGAAADPEATNR
                     KLRLLWIGCGKEDSLFPAAQAFDQFLKEHKIRHTFRESGGAHTWIVWRQYLYEVAPLL
                     FQ"
     gene            complement(9304..10686)
                     /locus_tag="VFA54_11895"
     CDS             complement(9304..10686)
                     /locus_tag="VFA54_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZS51559.1"
                     /translation="MTDQHQKIKEKYGLVRGFGLLEATALNMTNMVGVGPFITIPLII
                     AAMGGPQCMIGWVLGALLSLCDGMVWSELGAAMPGTGGSYLFLKESFRTSRLGMILPF
                     LFVWQFIVSGPLEVASGYIGFAQYADYFWPSWGPWENRTLTVAIGLVVLGLLYRRITA
                     IGKLTLLLWAGMLITVGWVIAGGLIHMAHFGAKLAFDFPPDAFRFSAGFALGLGRTML
                     IAMFDFLGYYDICYVGGEVRNPEYVMPRAIFYSVTGVALMYSVMNFCIISVVPWREAM
                     QSKLIVADYMSRLYGPRVAGAITILVLWTAFAACFAVLLGYSRIPYAAALDGYFFKVF
                     ARLHPKAKFPHISLLVMGGLSIVAGLLSLEWVVSALLTGRILIQFIGQIFAVHYLRKY
                     RPDIERPFKIWLYPLPSVIAFAGWTYIFLTSGWAFILFGLVTLGAGIGAFWLWRRSAA
                     EPVLSGAPGL"
     gene            11100..12374
                     /locus_tag="VFA54_11900"
     CDS             11100..12374
                     /locus_tag="VFA54_11900"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS51560.1"
                     /translation="MKKRYWLWAGVSAGVLFAACLGLAPGWAAPEAGSGGVIAFVGAR
                     IIDGTGKPAMQNATLVIRNGKIEAVGPSVKAPAGAQRIDATGKTIIPGLINAHGHVND
                     VSNLGVYARDGVTAVFSLGGNREIELRDQTRSEQQTASLTRARLYIAGPIPTSKTPED
                     GRKAVDALAAAKTDIVKFRLDDNLGRGSKMAPAVYSAIIDEAHKKGMRVAVHVVYLED
                     AKAVLRLGADYIAHSVRDHEIDAETIALLKKNQAFYCPTLTREVSTYAYAEAPAFLSD
                     PFFLKEADRGELAKVQDAKFRETMRNDQGAAWYKEHLAVAMRNLKKLQDAGVPVVMGT
                     DSGAPYRFQGYFEHMELEYMVKAGLSAMQALVAATGTAARCLKASDQFGTLEPHKWAD
                     LVVLTANPLDDIRNTRKIDSVWIAGNRVPAKN"
BASE COUNT         2651 a         3714 c         3791 g         2368 t
ORIGIN      
        1 gatgtgggcg atccgcgcca tatgcgtttg accgacggcc ggctgtttga ttatccgtgg
       61 atctacgcta ctcagacagc ctggtgggac ctggacggta gcgaaacttc acggctgaga
      121 gaataccttc tgcgtggggg attcctggtg gtcgatgact tctgggggcc ggaacagtgg
      181 cggctttttc gggaaacgat ggatcgggtc ctgccggatc aacccatcac cgacattgcc
      241 gagtcggatc cggtgatgca cgtgctctac gacattcagg aaaaagaccg tactttcatt
      301 cccggttccc gccatctctg gcgcggccct ggcggcacgg ttcagattgt gcagccgccc
      361 ggaacgattc ccgcctggcg ggccatgtac gactccaaga accacatgat cgtcgcgatc
      421 aatttcaaca cggatgtggc cgatgcgtgg gagttcgccg acgtgcctta ttatccggag
      481 gcgatgaccg cccttgccta tcgctacggg ataaactaca ttatttactc aatgacgcat
      541 tgacgctggc ccccggcatt tcccggcgcc gcggggtggt tgcaactctt tccaaacgct
      601 gaccagcacg aatacaagaa aacagatgaa cagaccgcga atcgcttagt gctttgccgg
      661 agcacgctcg atcaccatta atgtaaaggt tccgggcagc accgtccttc tgcccctgcc
      721 ccctgcggga gcttccccaa acttcgcgga aacgaggaac acgcggtgat tgatggggtc
      781 gagtcccata ttgcgcgagc cctggggcgt tggcacattt tcgaccacat gatattggtc
      841 cggagcgtcc tgatgaatca cagtaagggc gccgttcgca ttcgaggcaa acacatcgcc
      901 tgaagcggca tcatacccgg cgccgtcgac gccagtgcca attgggacag tcgctaccac
      961 cttgccggtc tggtagtcgg agaccgccat cacaccgctg cggcatccgc tgaaaagccg
     1021 atggtggacg gtgtcgatgg ccatggacac cggttgcttg cagggagcgg tcggccaacg
     1081 ccgcgataca gtcgccgcct ttgcgtcgat ctcgaccacc tcgctaatat cggtgaggtt
     1141 tgcgtatacc tcgccgtcgc cggccggcgc gccatattcc ggtttgccgc ccagtggaat
     1201 gttcgtgatc aattttcccg cgactggatc gatcacggtc gaggagtggg cgtcgccgtt
     1261 cagcgtgaag acgcgattcg acgcgttgtc gtatatgatc gcatcggcat cctcggcggc
     1321 agggatccgg ccgagaggct tgaaggtttt caaatcgaac atgaccacgg actggtcgtt
     1381 gcccgaggta gcgaatccat ggccagcagc aggcacaagc gccgttccgt gtgcgccgtt
     1441 gatgccggta acttcgccaa gcagtcttcc gttgtcttca tcgacgacca tcagacgatt
     1501 ttgccgggcg ataaacaggc ggtgattggg tgggtcgggc acgatatagt cccagccgcc
     1561 ctccccgccc agcatgtagg tgtgggtgac tcggtacgat ggcgactgcg cgaaaacagc
     1621 cgtcagaaca gccagtacga ggaatagcac tacggtgcgc atggtgctcc tttcgggctg
     1681 agtatacctt agcctgcatt cgtccgagcg gacctgcgaa gccatccata acgaaggatc
     1741 tcccaaaagg ctggcgctat atcgagtgga acccaaatgc cacctgcggc cgagaccaga
     1801 actacaacgt gtcaaaaatc gactcggccg cgtcacagtc cgcggtcggc acggacagtt
     1861 acgcaaggcc attcaagccg tcgccgccgc tttggatgaa gccggaaaac ctctctgctt
     1921 aaaactgccg aggaatccag ccgccgcgat cttcaggctc agaggccacc tagacgaggc
     1981 ggctgcaaaa tcggttgccg agtaacccga ctttcccagg gcgccgcgag aggtatgggc
     2041 gtttttccgg ctgcgatcat ttacaattac aatggtcgcc aggatcaaaa ttgggatgca
     2101 gccatccacg aggggcggga tgtccaccat gcggaacagc tttttcagtt tttttgtttt
     2161 gttggcttca gcgggggcgc tttttgcgca gaaagtggac gtgcaggagc tgcacctcga
     2221 taacggcatg acgtttctca tggcgccgcg caaaggagat ccgaatgtcg cggcgggctg
     2281 ggtggcccga gtgggttcgg taaacgagcg gccgggcatc accggcatat cgcacctctt
     2341 cgaacacatg atgttcaagg ggacgcggac catcggtaca acaaacatcg aggaggatct
     2401 caaaatcctg aatgaaatgg atcgggtgca cgagcagatc cgacaggagg aacaggctct
     2461 cgttgagcgc taccggcgcg gtgaaatcga tgacccttac aatcccaagt accgcacgcc
     2521 gcgtcattcc caactgatcg agcagtttga agctctcacc aagcgcgagc gccagtacac
     2581 catcaaaaac gagtttgatc ggatctaccg caacgcgggc ggctccggta tgaacgcagg
     2641 cactgattac gactacacgg tgtacttcat caatgtgccc tcgaacaagc tggagttatg
     2701 gttctggatg gaatcggacc gcttggcaaa tccggtattt cgcgattttt acaccgagcg
     2761 cgaggtagta catgaagagc ggcgcctgcg caccgattcc acgccgaccg gccgctttca
     2821 agagcagttt gacagcctct tttgggaatc ttctccttac gcgtggccgg tagttggctg
     2881 gcccagcgac ctggaaagct tcaccaaaga agatgcgctg gcgtacttcc aaacctacta
     2941 cgctcctaac aatctgacgg cctgcctggt gggagatttc gatccgccga aggctgcgga
     3001 actggccagg gcctactttg gacgcttaaa acgcggcacg cgagctcccg agccggttcg
     3061 caccctcgaa atgaagcagc tagccgagca gcggatgatc gcctacgccg aaactacgcc
     3121 tcaggtgcga atccgctacc acaccgtgcc cgacaaccat gccgacgagc cggctctgat
     3181 catgctggcc catttactga gcggccgcac cggccggctt tacaagtccc tcgtgctgga
     3241 gcagcagatc gccactaacg cctccgcgag ttccaacggg ctgaagtatg aggggtattt
     3301 cgaattaacg ggcgtggcgc gccagggaaa agatccgctc gaagtagaga agggcattta
     3361 caagcagatc gaaaacttgc agcttactcc gccgcccgag cgggaattac aaaaagtgaa
     3421 gaatcagcag gcggcagccg acttccggcg cctgcaatcg aacttcggcc tgatgttgca
     3481 gctcctcctg cacgaagcgc aagggagctg gcggacaatc aacacctatc cggaaaaatt
     3541 gcaggcggtg acggcggcgg atatccagcg tgtggcgaag aaatattttt cccccgagaa
     3601 tcgcagcgtg gcgatctact acaccaaaac gcccgccaga acgacggagg cgaggaacac
     3661 aggaggcggc cagtgagact ctgcatgaaa atgcttgcga tgctttgcgg gatgggtctt
     3721 gtgtggcccg cccgggtggg gcaaatggcg gctgcggaaa taccggcgca cccgaagctg
     3781 ttgcaggtcg aaccactgcg ctatgatcct ccaaaggctg cacaataccg gcacgtactc
     3841 tcaaacggcg cagtggccta cctagtggaa gatcacatgt tgccgctggt gaatctctcg
     3901 gtgctgatcc ggacgggacg gtacctggac ccgccgcgta aaacaggtct cgccgccatg
     3961 gtgggggccg agatccgctc cggaggcacg aaaaatcgca gcgcccagga tttcgacgaa
     4021 gaggccgatt ttttagcggc gagcatcaac tccacggtcg cggacaccga gggcagggct
     4081 acgttgaatt gtctgagccg caatttcgac gccgcgctgg agttgttctt cgacatgttg
     4141 cgaaacccgc gtttcgacca gcagcggctc gctctgtcga agatcaggac cttgcagacg
     4201 ctcgcccggc gcaatgaccg gtcgaatgag gtggaatcgc gcgagttcgc caggctgctt
     4261 cgcgggccat cccatttctc cacagcacag gagacaaagg cgactatcga ggcgatcacg
     4321 cgcgaggacc tgctaacgtt tcaccagaag tactatcatc cttctaactt tattttcgcc
     4381 gtctccggcg atttcaatac gaaggacctg ttagcgaagc tggaagattt gatgcgcggg
     4441 tgggaagggc ccaaaatcga agcgcctccg gtcccaaaac cggattatgc gccccatccg
     4501 ggtttgtacc tgattgataa gcctgaggtc aaccagtgcc gggtgtcggt cggcgaaatc
     4561 ggaacgatgc gcgataaccc cgatcactac gcgctggaga tcatgaatca gattttgggc
     4621 ggcggggcgt tcacttcccg aattgggtcg cgggttcgcg tggaggaagg gctggcctac
     4681 agcgcgggga cgatgatgac ctttggcgtg tactacgaag ggctattccg gggatttttt
     4741 caatccaaga gcgcttcttg cgcagaagcc gccacgatcg tgctggagga gattcagaga
     4801 atacgcgcag agcaggtgag tcccgacgag ctcgccacgg ccgttaacta tgcagtggag
     4861 gttttcccgc gttacttcgc cacgcctgcc gccgtggcct ccaccttcgc caatgacgaa
     4921 tacacacacc gtccggcgaa ctattgggag acctaccgcg aacggctgcg ccgagtcacc
     4981 gccgaagacg tgctgcgcgt tgcccgaaca tatttacacc cggaccgcct ggtgattctg
     5041 gccgtgggca atgtcagcga cattttcaag ggcgatccgg accatcctca gtattcctta
     5101 caaaagctcg ccggcggtaa gccgatcgtg cggctgcctc tcccggaccc tgttaccatg
     5161 gtttacgctg gcgatccttt ggaaaaagcc aaatgaccgc tggatccggg cgtggccgga
     5221 acggggaacg ggctttcggc cggacggctg caacggaacc tttccggagc ctcttcgtat
     5281 atctggattt gggtgtaagg gcgcaaggag ctggctgcgg ccggtttccc aaaagctttt
     5341 ttcaaaaata aaccgaggct ggaacaagga tgttacggag atggctcagg tggtccctgc
     5401 gcgggctgat ggctgctgta acgggggcga ttctcttcat tctttaccta acgtatggcc
     5461 gcccgaaaat ggccgcgccg agcgcggttc gggcgcatgc cacgccggaa aggattgccc
     5521 ggggcgagca tttggtcgag accatatgcg attgcatggg ctgccacgcc ccgcgggatt
     5581 ttacccggtt cggcggcccg gtgattccgg agaccaaagg ggaaggcggg gtaatgccaa
     5641 tggccggatt gcccggaacc atcgtcgcgg ccaacatcac acctgacccg gaaacgggga
     5701 tcggcgcctg gacagacggc gaaattatgc gggccattcg cgagggggtc gataaaaacg
     5761 gacgagcgct gttccccctc atgccctata ccgattaccg atacatgagc gatgaggacg
     5821 tggaatcgat cgtcgcctat ctcagaactt tgcggccgat caaaaaacgg caacccgaga
     5881 cgaagatcaa ctttcccgcc aacctcttga ttaaattggc gcccgcgccg gccggccacg
     5941 tggccgcagc ggatcggagc gataaacacg catatggaaa gtatctggcg acgatagggg
     6001 gatgcttgga atgccacacg ccgctgcaaa aggggggccg ggatacgacg aagctcctgg
     6061 cgggcggccg cctgttccag ttccccgggg cctcggtccg cagcgctaac ataacgcccg
     6121 agaacgacac gggcatcggc gtttttacag aagaattttt cattcacaag attcggtctt
     6181 atgctcctta cgcggagaaa gaatcaccga aaacgggtcc ggaaagcttt acgctcatgc
     6241 cgtggctaaa cttttggcgg atgactgacg aggagctgtc ggcgatttac gcctacctgc
     6301 ggactgtgcc gcccgtgtac aacgccgtgc ccatccacga ttgaggtcaa ggtatgagca
     6361 ggatcttgcc ggtcgtgcgg cgtccggcga gttcctcctg cgccttggcc gcctgctcca
     6421 ggggaaatac gtgctcgatg cgcagtcgca gtttgccggc tgcaatccat ccgagcacgt
     6481 cgccggcacg gtccagcagt tcctcccggc tcgccgtgta atctcccaac cgggggcgcg
     6541 tgagaaacag cgacccgcgc tgcgacaaaa gaagcgggtc aaccggcgga acagggccgc
     6601 tcgcgttacc gtacagcacg agcatgcccc gggggcgcag cgatttcatg cttttttccc
     6661 aggtgctgcg gccgacggaa tcgtacacaa cgtcgaggcc cgcgccgccg gtaaaacgtt
     6721 ttgcttccgc ctcgaaatcc tgggccgtgt aaatgattac ctcatcggcg ccggcttcgc
     6781 gcgccagccg cgccttctct tccgtcgaga cggtgccgag aacgcgcgcg cctcgcatct
     6841 tggcgatctg gatcagcagc aggccgactc cgcccgccgc ggcgtgaacc agagccgtgt
     6901 ggcccggccg gagcgggaat gtgctggtag ccaggtaatg ggcggtcatg ccttgcagca
     6961 tggctgcggc cccggatcgc gcatccacac cctcgggcag tttgaccagg cgagcggccg
     7021 gcgctactgc atactgcgaa taggctccgg gaatgccggc gtaggccacg cgatctcccg
     7081 gctccacgtg cgtggcctca gggccgacag cctcgacgac tcccgccgct tccaggccca
     7141 gggtcagcgg gaggggcgct ttgtaaagtc cctgccggaa gtagatgtcg ataaaattga
     7201 cgcccgccgc ttcgatcttt accagcgcct ctcccggccc cggagtaggc atgggtatct
     7261 cttcgtactg gagcacttcc ggaccgccgg tctggtgtac gcgtatcgct ttcatattcc
     7321 gcattctaca agaagaaaag gcatgacgcc ggggacccgc ttgggaggct taaggtaaat
     7381 acctctcact gagtacccta gtgccttatc gcatttaggt gaagacaccg ccgcaattcg
     7441 gtacggcctc tctattttca ggaatgccag ccattactag aataggtcta cggcatcgat
     7501 aagctcggga ggaagcagtg ccagagacag tagtcgaagc agattttgtg aatcttcggg
     7561 gcgttcatcg cctggccggg gccatccttg tgcaagccat cgaagacatt cgctgcggct
     7621 caggccgccg tagagaggac gcgatccgtt gggtcaccga ttcgaatgaa gatcagttca
     7681 gcttcgtctt ctgctgccgg atcctgggcc gcgatcccga agaagtgcgg cgttttctgg
     7741 tgcggcagcg gattccggac tggcttttct cagccaggct tgccgaggtt gataccgcct
     7801 ctacatgatc ggacccgttg gatccgttcc ctttcgtaac ccgatctccc gccgaaacga
     7861 accttgccgc aaaccggaac acggcccggc gatcgttgac gggctgtact ccgttcgaaa
     7921 aggcagcttg ctatccgcat gccagggccc ggaagagccg atttgcgctt gaactggcca
     7981 acttcggata tacttttggc agcgtctgcg ggcggagcgg gcccgattcc cggcccgcgg
     8041 ccgcgggcaa aaggggaagt ggaaaggagc caggaaatga ggacacttgt tattgccggc
     8101 ctgcttctga gcctgggagc ttctctcttg tgggcgcaac aaggttccga agccgccggc
     8161 cgagcacctg gagcccgatc caggcaggcc gggctgccgg ttccggtcac gtcgcccgaa
     8221 gtgcatccgg accgccgcgt taccttccgc ttgttcgcgc ccggggccgc cgaggtcaaa
     8281 ctcactggcg aattcatgga aggtgaacgc gcgctgcaaa aagacgagaa aggcctctgg
     8341 acgataacgg tcggcccgct ggacccggag atatacaatt acaacttcac cattgacggc
     8401 gtgcgcacca tcgacccggg caacgcgcat gtaaagacag gctcgacgcc gagcacgatc
     8461 cagagcatct tgcatgtgca tggggacggt cctgctttct tcgacggcca gcccgtgccg
     8521 cacggggaga tccacaccca ttggtatcaa tccaaatccc tgaacagcct gcggcgcctg
     8581 accgtctata cgccgccggg ttacgagagc aatggccgga cgcggtatcc ggcgctttat
     8641 ctgtttcacg gcgccaatgc cgacgaaacc gcctggacgc ggctggggcg cgtgaacctg
     8701 attctcgaca atctgctggc cgctggcaag gccaggccct ttgtcgttgt aatgccgttc
     8761 ggctatggcg ttcctcccgg gtccacgcgg ccctcgctga ctgctcccgg acttggactg
     8821 tcggaaaatg gcaggctttt tgccaaagac ctgatcgaag acgtgatccc ctatattcaa
     8881 gcccagtacc gcgtttatac cgatcgtgat catcgggcga ttgccgggct ttcgatgggc
     8941 ggcgggcagt ctctgagcat tggcctgagt cgcctggatc tgttcagcca ggtgtccgga
     9001 ttcagcgccg cgctgcggca ggccgatttc gacaagacct tcggcggcgc agccgcagat
     9061 ccggaagcga ccaaccgcaa gctgcggctt ttatggatcg gctgcggcaa agaagacagt
     9121 ttgttcccgg cggcgcaggc tttcgatcaa tttctgaaag agcacaagat tcgccatacg
     9181 ttccgggaaa gcggcggagc gcatacatgg atcgtgtggc ggcagtattt gtacgaggta
     9241 gcgccgttgc tgttccagta ggcgccccgc gaagctgttc ccagaggaag acagcgggcc
     9301 acatcataag cccggcgcgc ccgaaagtac tggttcggcc gcgctgcggc gccacagcca
     9361 gaacgcgccg atgccggcgc ccagcgttac cagtccaaat agaatgaacg cccatccgga
     9421 ggtcaaaaag atgtaggtcc atccggcgaa tgcgatgacg ctcggcaagg ggtagagcca
     9481 gattttgaac ggcctctcga tatccgggcg gtactttcgc agatagtgca cggcaaagat
     9541 ctggccgatg aattggatca gaatccgccc cgtcagcaga gccgaaacaa cccattccag
     9601 gctcaagaga ccggccacga tagaaagccc tcccatgacc agcagcgaga tgtgggggaa
     9661 tttggccttg gggtgcagcc gggcaaaaac cttgaagaaa tatccatcca gcgccgctgc
     9721 ataaggaatg cggctgtagc cgagcagcac ggcgaagcag gcggcaaagg cagtccaaag
     9781 caccagaatc gtgatcgccc cggccacccg ggggccataa agcctgctca tgtagtcggc
     9841 gacaatcagc ttcgattgca tagcctcccg ccatggcacc actgagatga tgcaaaaatt
     9901 catgaccgaa tacattagcg cgacgccggt caccgagtag aagatcgcgc gcggcatgac
     9961 atattccgga ttacgaactt cgccgcccac ataacagatg tcgtagtagc ccaggaagtc
    10021 gaacatagcg atcagcatgg tgcggccaag gcccagcgca aagccggcag agaagcgaaa
    10081 agcgtccggc ggaaaatcaa aggcgagttt tgcgccgaaa tgggccatgt ggatcagccc
    10141 gcccgcgatc acccatccca cggtgatcag catcccggcc caaagcaaca acgtcagctt
    10201 gccgatagcc gtgatacgcc ggtaaagcaa tcccaaaaca actaacccga tcgcgacggt
    10261 aagagttcgg ttctcccagg gcccccacga gggccagaaa taatccgcat attgcgcgaa
    10321 gccgatgtat ccggacgcca cctccagcgg cccgctcaca ataaactgcc acacgaacag
    10381 aaacggcagg atcattccca accggcttgt ccggaaactt tctttgagaa aaaggtagct
    10441 gccgcccgtg ccgggcatag ccgcccccaa ctcgctccac accattccgt cgcagagcga
    10501 aagaagggcc cccagaaccc agccgatcat gcactgcggg ccgcccatgg ccgcgataat
    10561 cagaggaatg gtgatgaacg gcccgacgcc gaccatgttt gtcatgttca gggccgtcgc
    10621 ttccaggagt ccgaagccgc gcacgaggcc gtatttttct ttgattttct ggtgctggtc
    10681 cgtcatgaag taggcagcgg ccggtggagg aaaaccagaa cagggggccg agcccggggc
    10741 tctgccgcac gctcggcggc gcagctattc aagcgtctgg cccggatgcg gcagcgcccc
    10801 agtggcccgc aaataaacca gccagaagca gggtattccc ggcgcgttgg taagcctgtg
    10861 acggcgaggg gcggagatat tactcccggc agcttcgaca cgcgaggttc gtcgcttgaa
    10921 acactctagc cagaggctgc tgccaagtca agcaggcggg gtctgaaaaa cgttgggatc
    10981 ggcagcttgt gttgcccgtc gtgcaggttt agaattggga tgttctgaaa tcctttctgc
    11041 ctgcgccgcg cgcatgcgcg cccgcggcgg caggcggcgt ctggccgcgg aggatctcga
    11101 tgaaaaaaag gtattggctt tgggccggcg taagcgccgg ggtgttgttc gccgcttgtc
    11161 tggggctggc tccgggctgg gcggctccgg aggccggctc cggtggcgtt atcgcttttg
    11221 taggcgcccg cattattgat ggcacgggca agccggccat gcaaaacgcg actctcgtta
    11281 ttcgcaacgg caagatcgaa gcggtcgggc cttccgtgaa agcgcctgcc ggggcccagc
    11341 gcatcgatgc aaccggaaag accattattc cgggcctcat caacgctcac ggccacgtca
    11401 atgatgtgag caacctcggc gtgtatgctc gcgacggcgt cacggcggtc ttcagtctcg
    11461 gcgggaaccg ggagatcgag ttgcgcgacc agacccgcag cgaacagcag accgcatcgc
    11521 tgacgcgggc tcgtctttat atcgccgggc caattcctac ctccaagacg cccgaggatg
    11581 gccgcaaggc ggtggacgcg ctggcggccg cgaaaacaga catcgtgaag ttccggctgg
    11641 acgacaattt gggccgcggc tcgaaaatgg cgccggccgt ttacagcgcg atcatcgatg
    11701 aagcgcacaa aaaaggaatg cgcgtagccg tccacgtcgt gtatttggag gatgcgaaag
    11761 cggtgctgcg gctgggagcc gactacatcg cgcacagcgt tcgcgatcac gagattgacg
    11821 ccgagactat cgccttgctg aagaagaatc aagcgttcta ctgccccacc ctcacgcgtg
    11881 aggtttccac atacgcctac gccgaggctc ctgccttttt gagcgacccc ttcttcctta
    11941 aagaggcgga ccgcggcgag ctggccaagg tgcaggacgc caaattccgc gaaacaatgc
    12001 ggaacgatca gggcgccgcc tggtacaagg aacatctggc ggtcgccatg cggaatctga
    12061 agaaactcca ggacgccggc gtgccggtgg tcatggggac ggacagcggc gccccctacc
    12121 gcttccaggg ctacttcgag catatggagt tggagtacat ggttaaggcc gggctgtctg
    12181 caatgcaagc ccttgtggca gcgaccggga cggccgcccg ctgcctgaaa gcctccgacc
    12241 agttcggcac tctcgagccg cacaaatggg cggatctggt ggtgctcacc gccaacccgc
    12301 tcgatgacat ccggaatact cgcaaaatcg attccgtctg gattgcaggg aaccgcgttc
    12361 cagccaagaa ttgattggac tcgacggcgc cggaggccct tatttctttg ggggcgcagg
    12421 cggcaggtct gcatcttccg cttgtttcgc cgcgctcaca gccgcacaat ttttcgggaa
    12481 cggaacgccc gcagcacgtt ccgggtgtcg atgataaggg gcgc
//
LOCUS       DASREK010000228        15818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1097502, whole genome shotgun sequence.
ACCESSION   DASREK010000228 DASREK010000000
VERSION     DASREK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 15818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15818
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097502"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1941)
                     /locus_tag="VFA54_11905"
     CDS             complement(64..1941)
                     /locus_tag="VFA54_11905"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZS51561.1"
                     /translation="MATTAANRQLITALEDEATRLRILSIRATSEAGSGHPTSCASAA
                     EIVSTLFFSVMRYDPANPGDPANDVFILSKGHAAPLLYAAWADAGFLTEGRLLTLRRI
                     DSDLEGHPTPRLPFVDVATGSLGQGLPAGIGMALCARYLDHSDQRIYVLLGDGESAEG
                     SVWEAAAIGSHYKLSNLCATIDVNRLGQSGPTMLEHQMDVYEARWRAFGWQALAVDGH
                     SVPALLEAYEKAARVTDRPTVVLARTIKGKGIPFAEDRNGWHGKALKKGEEENSAIHA
                     LESQLHDGRPGPWTPRKPPRRAPSRPEVGPPSTRPPYSIGDMVATREAFGAALAALGE
                     GNPLLVALDGDVKNSTFSEDFQKRFPDRFFEDFIAEQNMIGAAMGLAARGKIPFASTF
                     ACFLTRAYDFIRMAAISSSNIKLVGTHAGVSIGEDGPSQMGLEDLAMMCAEPNMTVLY
                     PADGVSAWRAVENAAGIAGPVYIRAGRPKTPVIYGPDEKFAAGRCKILRRSGRDRVTI
                     VAGGVTVYEALKAHDQLAREGIAVRVIDLYSVQPIDQEALQQSARETGGAVITVEDHY
                     AHGGIGDAVLAALAEEPEVKLRKLAVREIPRSGKPDELIARFGISAGAIVEQVRQMAA
                     K"
     gene            complement(2190..3248)
                     /gene="mtnA"
                     /locus_tag="VFA54_11910"
     CDS             complement(2190..3248)
                     /gene="mtnA"
                     /locus_tag="VFA54_11910"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZS51562.1"
                     /translation="MIRTIEWTPEGVVLIDQTRLPRQEVYVTCKTYEDVARAIRNMII
                     RGAPAIGVAAAMGVALGMQSATGDSADLDRRLEAICSTLAATRPTAVNLFWAIERMKR
                     AYALARGQGTEAVRAALVQEALAIYAEDIAINERMGRIGAALIPDSKTVLTHCNAGAL
                     ATAGYGTALGVIRAAVSAGKRIDVMADETRPYLQGSRLTVWELTQDGIPTTLITDNMA
                     GHFLKSGRIGCVLVGADRIAANGDVANKIGTYSLAVLARENQVPFYVAAPISTLDLAL
                     ASGDEIPIEQRSPAEVAHCGGVAVAPEGVVVENPAFDITPHRYVTAIITERGIARPPY
                     LQSLGELAGAPAPPNFRP"
     gene            complement(3251..4123)
                     /locus_tag="VFA54_11915"
     CDS             complement(3251..4123)
                     /locus_tag="VFA54_11915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZS51563.1"
                     /translation="MNIDPHTQPGADAARSAEGCAEAAHGRLPELRGTQQPPPSALPD
                     LEPGSWTYWDVLVVIGFALAAQIIANIGVMFVVLLVGEARGGRFNLVDLMMNPAVLLP
                     VQLIWWVLVFWIIYRIVRARDPRPFPQAVRWVRPHLPPGIYLGGGALLAVSVAMLSYL
                     LPSPNRKMPMEELFRDPTSAFLLAAFGVFAAPPVEELLFRGFLFPVVERSHGQIAAVV
                     ATAALFSLVHAQQYGWAWQNLLLLGYVGVIFGTVRAVSRSLIPSTLMHAAYNFTLFAG
                     LYAASKGFHNFNFR"
     gene            complement(4139..6901)
                     /gene="uvrA"
                     /locus_tag="VFA54_11920"
     CDS             complement(4139..6901)
                     /gene="uvrA"
                     /locus_tag="VFA54_11920"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZS51564.1"
                     /translation="MIDHITVRGARQHNLRNIDVEIPRDSLTVVTGVSGSGKSSLAFD
                     TIYAEGQRRYVETLSPYARQFLDQMERPDVDLIEGLSPAISIEQRTTSRSPRSTVGTI
                     TEIYDYLRLLYAQIGVPHCPQCGRQISRQSVEQIVGQVLNLCAGERIMILAPIVRGRK
                     GEFKKDLEKLARQGFLRARIDGVLQSVEDEIKLDKRRNHTIEAVIDRLVIKPGVDKRL
                     EASIQTATRLTGGIVLVAVVGGDERLYSEKLACVDCGASVPQLEPRSFSFNSPYGACP
                     GCAGLGSKWAFDPAKIIADPSKPLLSGGLAPAASSVYVQSSLQEIAESHRIDLRKPFE
                     ALPKKAQNLLLYGGNGAEGILQILQSAYDDSGSESYREWLTQYMSSAPCPDCKGKRLK
                     PESLAVKVGGLSIDQFTALPAARALEFIRNLRLAGREEKIASKIVEEIAKRLEFLSTV
                     GLNYLSLDRSAATLAGGEAQRIRLATQIGTRLRGVLYVLDEPSIGLHPRDNTRLLETL
                     EQLRNLGNTIVVVEHDQETIERADYVLDLGPGAGRLGGALVAAGTPREIAQNPNSLTG
                     QYLAGMRAIEIPEKRRRPNGKVLAILGAEEHNLKNIDVEFPLGLLTVVTGVSGSGKST
                     LVNEILYRALACHLYRSIEEPGRHREIRGIEQVDKVVRIDQSPIGRTPRSNPATYTGV
                     FTLIRDLFAMLPESRERGYRPGRFSFNVKGGRCEACQGDGQKRIEMNFLPDVFVTCDV
                     CRGRRYNHETLEVKFKSYSIADLLDATVEEARSVLENIPQVAAKLETLCQVGLDYIHL
                     GQPATTLSGGEAQRIKLARELSRRQTGKTLYILDEPTTGLHFDDVRKLVDVLQQLVSL
                     GNTVIVIEHNLDVIKSADWIIDLGPEGGDAGGRVVASGTPEQIVRGRKGHTAAALARV
                     LNGKAR"
     gene            7185..7505
                     /locus_tag="VFA54_11925"
     CDS             7185..7505
                     /locus_tag="VFA54_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HZS51565.1"
                     /translation="MPKYLVQANYTAEGLKGLMKDKGSGRQAAVSKAITGLGGKLEAF
                     YFSFGETDAVVIADLPDNVSAAALSLQVCASGLAATKTTPLLTVAEIDQALQKSVDYR
                     PPGR"
     gene            complement(7551..8057)
                     /locus_tag="VFA54_11930"
     CDS             complement(7551..8057)
                     /locus_tag="VFA54_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51566.1"
                     /translation="MLSSKLIQLIEDHWEQITAAAIQQIRADPDLPHMRELMVSELRA
                     WHSVILRALRSWNSAQKDGELAQQYEELGRRRFEETVPLHETIRSLHILKHRMVDFVR
                     NQGFGRTSLELYAEEELEHRIGLFFDCLIYYVVRGYENAVRSAAPAAFQPSPKSAAAQ
                     INWDIPYL"
     gene            8330..9169
                     /locus_tag="VFA54_11935"
     CDS             8330..9169
                     /locus_tag="VFA54_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZS51567.1"
                     /translation="MSFRKTCRGFGIAAAFLGTLYAFQLPFREYPGVEYNDFPLPPNW
                     QEDGEWVFARLMYPSAPTARYGRRYLIGGGDWTHGMTSWTQDYPRADRHFAEELRRLT
                     RIQVRSVEQPVNLDDGDEVFYYPWLYAVQVGEWKLTEAQASKMREYLLRGGFFMCDDF
                     WGEAQWQVFLQSMQRVFPDRPVVEIDDNDAIFHTVYNLQNRYQIPGQRYVWTGVTEKC
                     YGCPPHWRGIYDDKGRLMAAMCFNSDVGDSWEWADDPRYSERFSGLGMRLGVNYVVYA
                     MTH"
     gene            9299..10123
                     /locus_tag="VFA54_11940"
     CDS             9299..10123
                     /locus_tag="VFA54_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZS51568.1"
                     /translation="MKPRIALFGLLWGVTLAGILGALQLPFREYPGFEYYGFPLPPDY
                     MQPGEWTFARLMYPQTRYGRYRWFSDWTKGMSSWTIDYPRSDRHLAEEVRRLTRIDAR
                     SVEQPVNLDDGDEVFYYPWLYAVEVGHWELTDSQALKLREYLLRGGFFMVDDFHGTRE
                     WAVFVASMRRVFPDRPIVEIPDNDPIFHTVFDLSDRFQVPGAQYIYSGRTFEFDGVQP
                     HWRGIYDDKGRVMVAICFDMDLGDSWEHADNPEYPEKFSGMGMRIGINYIVYAMTH"
     gene            complement(10164..10511)
                     /locus_tag="VFA54_11945"
     CDS             complement(10164..10511)
                     /locus_tag="VFA54_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51569.1"
                     /translation="MRNAWLAALMMAAILILIAPQQANAGVRVGIGVGVVAAPVYPYP
                     AYAPYPCVYPYPYPCVYPYPYYYPYPYGYVAPAYVYPYGGVFFRGFVGGRRVVVRGPV
                     VVRGRAFHGVRRR"
     gene            complement(10575..11621)
                     /locus_tag="VFA54_11950"
     CDS             complement(10575..11621)
                     /locus_tag="VFA54_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51570.1"
                     /translation="MRSVSFCPIRAAAYGLMLYAAAAGLIFAQNTPDAAPQEQNAAPH
                     RGWRRIGDPPPQAADGATDPAGPNYAQSPPQDSNQPPVVSAPADPPQYGIPPQLTIKA
                     GTFVTVRVNQVLSSDRNEPGDAFSATLVRPIVVDGIVVAQRGQTVGGRVAEAKKAGRV
                     EGVSRLGIQLTDLSLVDGQQVPIQSQLISRSGPTSEGRDAAGVAATTATGAVIGAAAD
                     WGRGAAIGAGAGAAAGVLGVLLTRGRPTVIYPESVLTFRLEAPVTISTERAPQAFRYV
                     DPNTDYQRPSYEARAQTPPPPACGPYACPPPPPPYYGPYYYPYPYYYAPGFAVFYGPR
                     FYYGHRLAYRAYRR"
     gene            complement(11880..12632)
                     /locus_tag="VFA54_11955"
                     /pseudo
     CDS             complement(11880..12632)
                     /locus_tag="VFA54_11955"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            13114..14199
                     /locus_tag="VFA54_11960"
     CDS             13114..14199
                     /locus_tag="VFA54_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS51571.1"
                     /translation="MLSLGALLPAGLRSQSAAQQAPQEQEGRPVATFGSDVKVVNLFV
                     TVRNKQGQIVRDLKKEDFILEEDGRPQTIRYFSQESDLPLTLGLVVDTSLSQRRVLGG
                     ERNACYVFLDEMLRDKDMAFVIHFDREVELLQDLTSSRKQLEAALERLETPQLSRRPQ
                     WGGRGRPGSPGPSGTPGRRGVGTTLYDAVFLACDELMKKQSGRKALILLSDGVDMGSK
                     VSLPEAIESAQRADTLVYSILFSDQQAYGSPGGFGSRGGWGGPGMGRRGRMGGGRGFP
                     QPQARPDGKKILERISKETGGALFEVSKKESIGQIFDRIQEELRNQYSLGYSPDSNAG
                     AGYRKIHLTTRQKNLIVQTRDGYYAGA"
     gene            14482..14685
                     /locus_tag="VFA54_11965"
     CDS             14482..14685
                     /locus_tag="VFA54_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51572.1"
                     /translation="MLEDVDKCKRKGILINTFLLASDYGLVQFLQKVTEICPGGKAYF
                     TTRYNLGQYLPMNYRDRKMRAIH"
     gene            complement(14730..>15818)
                     /locus_tag="VFA54_11970"
     CDS             complement(14730..>15818)
                     /locus_tag="VFA54_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51573.1"
                     /translation="TWNNTPYMQTWNFGLQRKLKGNMLLDVNYLGTKGTHLYFGGAGS
                     LNYLGSWVESATPDQITQLNSFVANPFYGIITNPASGLSAATVQQSQLLKPYPQFSGF
                     SGNDPPSADSIYHSLQVRLEKRFSSGLQLLATYVFSKSIDDSSVACSCTTWLGGATSL
                     QDPNKRFLERSVSQFDIPQVLQFSYVYQLPFGRGKHWGGRWNSFVNAALGGWQTNGLW
                     RFDNGMPLALSVSTSRALPTYGPQRPNLLGPLTRNNGSNWLTQYFANPQNAVTPAPFT
                     VGNAPREISTARAPGTSTSALSMFKQFALPRLHEGSMVEFRAEAFNALNHPQFSPPNL
                     TVGSSAFGKVTSQANSPRQVQLALKLYF"
BASE COUNT         3125 a         4878 c         4800 g         3015 t
ORIGIN      
        1 tcacgcgccg ccgggcgctg aagtcgttga gcctggccgg tgaccccagt tgggagaagg
       61 cctttacttg gccgccattt gcctcacttg ttccacaatc gccccggccg agatgccgaa
      121 acgggcgatc agctcgtcgg gtttgccgct gcggggaatt tctctcaccg ccagcttgcg
      181 gagcttgacc tcgggctctt ctgcgagggc cgccagaacg gcgtctccaa tgcctccgtg
      241 cgcataatga tcttccacgg tgatcactgc gccccctgtt tcgcgggccg actgttgtaa
      301 cgcctcctga tcgatgggct ggacgctgta aagatcaatc acccgcaccg cgattccttc
      361 cctggctagc tgatcgtgag ccttcagcgc ctcatacacg gttacgcccc ccgctacgat
      421 cgtgacgcgg tcgcggccgc tccggcgcag aattttgcat cggcccgctg caaacttctc
      481 atccgggcca tagattacgg gggttttcgg ccggccggct cggatataga cgggtcccgc
      541 gatgccagcg gcgttttcga ctgcacgcca cgcgctgact ccgtccgccg ggtagaggac
      601 cgtcatattc ggctcggcgc acatcatggc caggtcttcc aggcccatct gggaggggcc
      661 gtcctcgccg attgaaacgc cggcgtgcgt gcccaccagc ttgatgttgc tgctgctgat
      721 cgccgccatg cgaatgaagt cgtaagcgcg agtcagaaag caagcaaagg tggaagcaaa
      781 cggaattttc cctcgggccg cgagccccat cgccgctccg atcatgttct gctcggcgat
      841 aaagtcttcg aaaaacctgt ccggaaacct tttttggaaa tcctcgctga aagtcgagtt
      901 ttttacgtcc ccatcgagcg cgaccagaag cgggttccct tcgccgagcg ccgccagggc
      961 agctccgaaa gcttcgcgcg ttgcaaccat gtctccgatc gagtaaggcg gtctcgttga
     1021 gggcggccca acttccggac ggctgggcgc ccggcgcggc ggtttgcgcg gcgtccacgg
     1081 gccgggacgc ccgtcatgaa gttgcgattc gagcgcatga atcgcgctgt tctcttcctc
     1141 gcctttcttg agggctttgc cgtgccagcc gttgcgatct tcggcaaaag ggatgccttt
     1201 gcccttgatg gttcgcgcca gaaccaccgt gggccggtcc gtgacgcgcg ccgctttctc
     1261 atacgcttcg agcaaggcgg ggacgctgtg gccgtccacc gccagcgcct gccagccaaa
     1321 cgctcgccag cgggcttcgt aaacatccat ttggtgctcg agcatagtgg ggccgctttg
     1381 tcccagccgg tttacgtcga tcgtggcgca gagattgctc agtttgtaat ggctgccgat
     1441 cgcggcggcc tcccaaaccg atccttccgc cgactcgcca tcgcccagca gcacgtaaat
     1501 cctctgatcg gagtgatcga gatacctggc gcatagagcc atcccgatgc ccgccggcag
     1561 tccttggccg agcgatcccg tcgccacgtc cacgaagggc aggcggggcg tcgggtgacc
     1621 ttcgaggtcg gaatcgatgc gtcgcagcgt cagcagccgc ccctcggtaa gaaaaccggc
     1681 gtccgcccag gctgcgtaga gaaggggcgc agcatgcccc ttggagagta tgaagacgtc
     1741 attggccgga tcgccgggat tggcgggatc atatctcatg acggagaaaa acagcgtcga
     1801 gacgatctcc gccgcggaag cgcagctcgt aggatgcccg ctgcccgctt cactggtggc
     1861 acggatggaa agtatccgca ggcgcgtcgc ttcatcctcc aatgcggtga tgagctgtct
     1921 atttgccgct gtagtggcca taagcttgtc cctttccgcc gccggccctc tctcaaacca
     1981 gccgccttcc ctagcatcat agcgccaacc gccggaaggc cccgccgtga gatgcatcac
     2041 agtaagaatc ccgccggatc agaaggcgct tcacgccagc gccgcccggc ggcgctgcct
     2101 gcgattccgc tcagcggctg atcgcgttta cgaccctgtg aggccggagg tttggtcaag
     2161 ggaatccggc gcgctcccgg cggaagcgct caagggcgaa aattgggagg ggccggggcg
     2221 cccgccagtt ccccgaggga ttggagatac ggcgggcgcg cgatgccacg ctccgtgatg
     2281 attgccgtta cgtatcggtg cggagttatg tcaaaggccg ggttctcgac gacaacgccc
     2341 tcaggcgcta cagcgacgcc gccgcagtgg gcgacctcgg ccggggaacg ctgctcaatc
     2401 ggaatctcgt ccccgctggc cagggccaga tccagagtgg aaataggtgc ggcgacgtag
     2461 aacggcacct gattctcgcg cgccagcaca gccagagaat acgtaccgat tttgttggcg
     2521 acgtcgccgt tggcggctat gcggtcggcg cccaccagga cgcagccaat gcggcccgac
     2581 ttcagaaaat gcccggccat gttatccgtg atgagggtcg tgggaatgcc atcctgcgta
     2641 agctcccata cagtcaggcg cgatccttgc aaatacggac gcgtctcatc ggccatcacg
     2701 tcgatgcgct tgcccgcgct gacggcggcg cggatcactc ccagcgcggt gccgtagccc
     2761 gcggtggcca gggcgccggc gttgcagtgc gtgagcaccg ttttggaatc gggaatcagg
     2821 gcggcgccga tgcggcccat tcgctcattg atcgcgatat cttccgcgta aatcgccagc
     2881 gcttcttgaa caagcgccgc gcgaacggcc tccgttccct ggccgcgggc gagcgcgtaa
     2941 gcccgcttca tgcgctcaat ggcccaaaac agattgacgg cggtcggccg ggtggcggcc
     3001 agcgtggagc aaatcgcctc cagtctgcgg tcaagatcgg ccgaatcgcc ggttgcggat
     3061 tgcatgccga gcgccacgcc catcgccgcc gccacgccga tggccggcgc cccgcggata
     3121 atcatgttgc ggatggcccg cgccacatcc tcatatgtct tacaagtaac atagacttcc
     3181 tggcggggca gccgcgtctg gtcaatcagg acgacgcctt ccggtgtcca ctcgatggtt
     3241 cggatcatgg ctatcggaaa ttgaaattgt gaaaaccctt gctcgccgcg taaaggccgg
     3301 cgaagagcgt gaaattatat gccgcatgca tgagcgtgga aggaatcagc gaacgggaaa
     3361 cggcgcgcac cgtcccgaag atcacgccga catagccgag cagcagcaaa ttctgccagg
     3421 cccagccgta ttgctgcgca tgcaccagag aaaacaaagc cgctgtcgcc accacggcgg
     3481 caatctgccc gtgggaacgc tccactaccg ggaacagaaa accgcgaaac agaagctcct
     3541 ccaccggagg cgccgcgaac acgccgaagg cagccagcag aaatgccgag gtggggtcgc
     3601 gaaacagctc ctccatcggc attttgcggt taggcgacgg cagcaaataa gagagcatgg
     3661 cgacggaaac cgccagtaga gcgccgccgc cgagatagat gcccggcggc aggtgcggcc
     3721 gcacccagcg gaccgcctgc ggaaacgggc gcgggtcgcg cgcccgcacg atgcggtaaa
     3781 tgatccagaa gaccaggacc caccagatga gctgcacggg caaaagtacc gccgggttca
     3841 tcatcagatc cacaaggttg aaccggcccc cgcgcgcctc tcccaccagg agcacgacga
     3901 acatcactcc gatattcgcg atgatctgcg ccgccagggc gaagccgatc acaaccagga
     3961 catcccagta cgtccagctc cccggctcca agtccgggag cgcggacggc gggggctgtt
     4021 gagttccccg cagttcgggc agccgtccgt gcgccgcctc ggcgcagcct tctgccgagc
     4081 gagccgcgtc agcgccgggt tgtgtatgag gatcaatatt cattcggggt tccgccggtc
     4141 agcgcgcttt tccgtttaaa accctcgcca gcgccgccgc tgtgtgacct ttgcgtcccc
     4201 ggacgatctg ctcgggcgtg cctgatgcca cgacccgccc tccggcgtct ccgccctccg
     4261 gacccaggtc tatgatccag tcggcgctct tgatcacgtc caggttgtgc tcaattacga
     4321 ttacggtgtt tcccaggctg acgagctgtt gcagcacatc caccagttta cgcacatcat
     4381 caaagtgcag cccggtagtt ggctcgtcga ggatatacaa ggtcttgccg gtttggcggc
     4441 ggctcaattc cctggcgagt ttgatgcgtt gagcttcgcc gccggagagg gtcgtcgccg
     4501 gctgtcccag atggatgtag tcaaggccga cctggcacaa cgtttcgagt tttgcggcca
     4561 cttgggggat attttccaac acagagcgcg cctcttctac ggtggcatcg agcaagtcgg
     4621 cgatggagta gcttttgaac ttgacctcga gcgtctcatg attgtaacgt ctcccccggc
     4681 acacgtcgca ggtgacaaat acgtcgggca gaaagttcat ttcgattcgc ttctggccgt
     4741 cgccctgaca agcttcacag cggccgccct tgacgttgaa gctgaagcgg cccggccggt
     4801 agccgcgttc ccgagattcc ggcagcatcg caaagagatc gcggatcagc gtgaaaactc
     4861 cggtgtaggt ggccgggttc gaccgcggcg tgcggccgat gggcgactgg tcgatgcgga
     4921 ccaccttgtc cacctgctcg atgccgcgga tttcccggtg acggcccggc tcctcgatgg
     4981 agcgatagag gtgacaggcc agcgcgcggt aaaggatttc gttcaccagt gtggatttgc
     5041 cgctgccgga tacccccgtg acaaccgtca gcaaaccgag cggaaactcg acatcgatgt
     5101 tcttcaggtt gtgctcttcg gctcccagga tggcaagcac cttgccgttc ggccgccggc
     5161 gcttctccgg aatctcgatg gcccgcatgc cggccagata ctgcccggtg agagagttcg
     5221 ggttttgcgc gatctcacgc ggcgttccgg ccgctaccag agcccctccc agccgcccag
     5281 ctcccgggcc cagatcgagc acataatcag cgcgctcgat ggtttcctgg tcgtgctcga
     5341 caacgacaat cgtattgccg agattccgaa gctgctcgag cgtctccagc aggcgcgtgt
     5401 tatcgcgagg atgcaggccg atggaaggct cgtcgagcac atataatacc cctcgcagcc
     5461 gtgtgccgat ttgcgtggcc aggcgaatcc gctgggcttc gccgccggcc agcgtcgccg
     5521 ccgaccggtc aaggctcaaa taattcaggc ccaccgtgga gagaaattcc aaacgcttgg
     5581 cgatttcttc cacaattttg gacgcgattt tttcttcgcg ccccgccagt ctcagattgc
     5641 ggatgaactc cagcgcgcga gcggccggca aggcggtgaa ctgatcgatg gagaggccgc
     5701 ccaccttcac cgccaggctc tccggcttca gccgcttgcc cttgcaatcc gggcacggcg
     5761 ccgatgacat gtactgggtc agccattccc gatagctttc cgagccggag tcgtcatagg
     5821 cgctctgcaa aatttgcaaa attccttccg ccccgttgcc gccatagagc agaaggtttt
     5881 gtgccttctt gggcagcgcc tcaaacggtt tgcgaagatc gatgcggtgg ctttccgcaa
     5941 tttcctgcaa agaactttgt acataaacgg aggaagccgc gggcgcaagg cccccgctca
     6001 gtaaaggctt cgagggatcg gcgatgattt tagccggatc gaacgcccac ttgctcccca
     6061 gtccggcgca gcccggacat gcgccgtagg gactattaaa cgaaaaagaa cggggctcga
     6121 gttgtgggac gctggcgccg caatccacgc aggccagctt ctcggaatac aaccgctcgt
     6181 ctccgccgac cacggcgaca agcacaattc cgccggttag tcgcgtggcg gtttgaatgg
     6241 aagcctcgag gcgcttgtcg accccgggct tgatcacgag gcggtcgata acggcttcta
     6301 ttgtgtggtt gcggcgcttg tcgagcttaa tctcgtcctc gaccgattgc agcacgccgt
     6361 caatccgcgc ccgtaaaaag ccctggcggg cgagcttttc gagatccttt ttgaattccc
     6421 cctttcgccc gcgcacaatg ggagccagga tcataatgcg ctcgccggcg caaagattca
     6481 aaacctgccc gacgatttgc tcgaccgact gccggctgat ttgccggccg cactgcgggc
     6541 aatgcggcac gccgatctgg gcgtagagca gacgcaggta gtcgtaaatc tcggtaatcg
     6601 tacccacggt tgagcgcggg ctgcggctcg tcgtgcgctg ctcgatggaa atcgccgggc
     6661 taaggccctc gatcagatcc acatcgggcc tttccatctg gtccaggaac tggcgggcgt
     6721 aaggcgagag agtttccaca taccgccgct ggccttcggc gtaaatggta tcgaaggcga
     6781 gcgacgactt gccggatccg ctaacgccgg taaccacggt gagcgaatcg cgtgggatct
     6841 cgacgtcgat gttcctgagg ttgtgctgcc gggctccccg gacggtgatg tgatcgatca
     6901 cggaaatgaa taggcgccgg agggctaatt tattatttta ccaagagcgc gctttccagc
     6961 agacacttcc ggtcgccggt gctttcgccg gcatccgcat tcttgttctt tgcttcgcct
     7021 ttttccgggt ttcggcagcc agctggattg cgtcgtgcat cagccttgct ctagcctcga
     7081 cgcaaaaccc ttcggcacaa aagctccttg acttcgcccg gccccagggc gtatggtggt
     7141 gccaactgat cggacctaaa actgattttg gagagggtat acgtatgcca aagtatctcg
     7201 ttcaagcgaa ttacaccgcc gaaggcttga aaggtctgat gaaggacaag ggatcaggcc
     7261 gccaggcggc cgtttccaag gcgataacag ggctgggagg caaactcgag gcgttctact
     7321 tcagtttcgg ggaaacggat gccgtggtga tcgccgattt gccggataac gtctcggccg
     7381 cggcgctgtc gttgcaagtt tgcgcgagcg gcctggcggc gaccaagaca accccgctgt
     7441 tgaccgttgc cgagatcgat caggcgttgc aaaagagcgt cgactatcgc ccgccggggc
     7501 gttgaggaaa cagcggaaac cggttccggc gcgccgcgat cacagatcgc ttagaggtaa
     7561 ggtatgtccc agttgatctg cgccgcggcg cttttcggag acggttggaa agccgccggc
     7621 gcagcgctgc gcactgcgtt ctcatagccg cgcacaacgt aataaatcag gcagtcgaaa
     7681 aacaagccga ttctgtgttc gagctcttct tcagcataca gctccagcga agtgcgccca
     7741 aatccctgat ttcggacaaa atcgaccatg cggtgcttca ggatatgcag gctccggatc
     7801 gtttcatgaa gcggcaccgt ttcctcgaag cgccgccggc ccaattcctc atactgctgt
     7861 gcaagctccc catccttctg cgccgaattc cacgaacgca gcgctcttaa aatcacagaa
     7921 tgccaggcgc ggagctcgga taccatcagc tcgcgcatgt gcggcaagtc cgggtcggcc
     7981 cgaatttgct ggatcgcggc tgcggtaatt tgctcccaat ggtcttcaat gagttggatc
     8041 agcttgcttg ataacatcca gtgcctcctg tgcccgtaca aactcctgcg gcaagaaatt
     8101 acccgcaggg agtgacttgg gctggtccct cactttttag acgcgtcacg gagccggggc
     8161 gactgaaggc gcgccggctc gacggcatcg ttccgcacat ctggcgccaa aaggcctcgc
     8221 attcgcctgt gcgcctacga caggacggca gctctccagc ctccctgcgc cggcgcgctc
     8281 cactcgcggc ggcgcggttg cggtggtatc ctcaggcgag gcgacgctcg tgagtttccg
     8341 caagacttgc cgcggtttcg gaatcgccgc tgcgtttctg ggaacgctct acgcgttcca
     8401 gctcccattc cgggaatatc cgggtgtgga gtacaacgat ttcccgctcc cgccgaactg
     8461 gcaggaggac ggcgaatggg tgttcgcacg gttgatgtac ccgtcggccc ccaccgcgcg
     8521 ttacggacgc cggtatctga taggcggcgg ggattggacg cacggcatga cgagctggac
     8581 gcaggattat ccccgcgccg accgccactt cgccgaggag ctgcgccgcc tcacgcgcat
     8641 ccaagtccgt tcggtggaac agccggtgaa tctggatgat ggcgacgagg tcttctatta
     8701 tccgtggctc tacgccgtcc aggtcggtga atggaagctg acggaggccc aagcgtcgaa
     8761 gatgcgcgag tatctgctgc gcggcggctt tttcatgtgc gacgatttct ggggagaggc
     8821 gcagtggcag gtcttcctcc aaagcatgca gcgggttttc ccggaccgtc cggtagtgga
     8881 gatcgacgac aatgacgcca tttttcatac cgtttataat ctccaaaacc gctatcagat
     8941 ccccggccag cggtacgtgt ggaccggcgt tacggaaaag tgctacggct gcccgccgca
     9001 ctggcgcggc atttacgacg acaaaggacg gctgatggcg gcgatgtgct ttaactccga
     9061 cgtgggcgac tcgtgggaat gggccgacga tccccgctat tcggaaaggt tctcgggact
     9121 cggcatgcgg ctcggcgtga actatgtcgt ctatgctatg acccattgac acgtccaatg
     9181 cgctttttcg ccacccagtc aaaatcgtgc gccgaaatcg gtctccgttg aaagcgccgc
     9241 ccggcaaatt tttccggcga gctatactca atagcgagaa gtccggcgga tggcattgat
     9301 gaagcctcga atcgctcttt tcggtctcct gtggggagtt acgctggcgg gaattctcgg
     9361 cgccctccag ctgcccttcc gcgagtatcc gggatttgag tattacggat tcccgctccc
     9421 gcctgactat atgcagcccg gggaatggac gttcgcgcgg ctgatgtatc cccagacgcg
     9481 gtacgggcgc tatcgatggt tctcggattg gacaaaaggc atgtcaagct ggaccattga
     9541 ctatccgcgc tccgaccggc atctggccga agaggtccga aggctcaccc gcatagatgc
     9601 ccgctcggta gagcagccgg tcaatctcga cgacggcgac gaggtgtttt actacccgtg
     9661 gctgtatgcg gtcgaggtcg gccactggga attgaccgat tcgcaggcgt taaaattgcg
     9721 cgaatacctg cttcgcggcg gattcttcat ggtcgatgat ttccacggca cgcgcgagtg
     9781 ggcggttttc gtagcgagca tgcggcgcgt gtttcccgac cgccccatcg tcgaaatccc
     9841 cgataacgat cccatctttc acacggtctt cgatctcagc gaccgttttc aggtgcccgg
     9901 cgcgcaatac atctacagcg ggcgcacctt tgaattcgac ggagttcagc ctcactggcg
     9961 tggaatctac gatgataaag ggcgggtgat ggtcgcgatc tgcttcgata tggatctcgg
    10021 cgattcctgg gagcacgcag acaacccgga atatcccgag aaattttccg gcatgggcat
    10081 gcggatcggg atcaactata tcgtctacgc gatgacccac tgacgggctg ctcgcaggcg
    10141 ccacgaccgg cgttcgcctg gccttagcgg cggcgaactc cgtggaaggc acgcccgcgc
    10201 accacaaccg gccctcttac tacaacccgg cgccctccta caaatccccg aaagaagact
    10261 ccaccgtacg ggtaaacata cgccggcgcc acatacccat aagggtacgg gtagtagtac
    10321 ggatacgggt acacgcaagg gtaagggtat gggtacacgc atggataagg agcgtacgcc
    10381 ggataagggt aaacgggggc ggcgacgacg cccaccccga ttccgactcg cacaccggca
    10441 ttggcctgct gcggcgcaat cagaatcaga atggcagcca tcatcagagc cgctaaccag
    10501 gcatttctca tactcaagtc cctcctaaaa cttttatcca cgccccaaaa agaacggagc
    10561 caatcctccc ctctctaacg gcgataagcg cgataggcaa gccgatggcc gtagtagaaa
    10621 cgcggcccat agaaaaccgc aaatccaggc gcgtaataat agggatacgg ataatagtac
    10681 ggcccgtaat acggaggcgg gggcggcggg cacgcatagg ggccgcaggc aggcggcggt
    10741 ggggtctgcg cccgagcctc atacgatggc cgctgataat ccgtattggg atcgacatag
    10801 cggaaagcct gcggcgctcg ctccgtcgag atggtgaccg gcgcttccag acggaacgtc
    10861 aagacagact ccggataaat caccgtcgga cggccgcgcg tgagcagcac gccgagaacc
    10921 ccggctgcgg cgcccgctcc ggctccaatc gccgctccgc ggccccagtc ggcggcagct
    10981 ccgatcactg ccccggtcgc cgttgtagcc gccactccgg ccgcgtcgcg gccttcggaa
    11041 gtaggcccgc tgcggctgat caactgggac tggatgggaa cctgctgccc atccaccaga
    11101 ctgaggtcgg tcaactgaat gcccagccgc gacacgcctt ccacacgccc ggcttttttc
    11161 gcttcagcaa cgcgcccgcc caccgtttgg ccgcgttgcg ccacaacgat cccatccaca
    11221 acaatcggcc gcaccaatgt ggccgagaac gcatcgcccg gttcattgcg gtcggacgaa
    11281 agcacttggt ttacgcgcac ggttacaaac gttcccgctt tgattgtcaa ttgtggcgga
    11341 atcccgtact gcggcggatc ggccggcgcc gaaacgaccg gcggctggtt ggaatcctgc
    11401 ggcggactct gggcgtaatt cggaccggct ggatccgttg cgccatcggc ggcctgcggc
    11461 ggaggatccc caatgcgccg ccatccgcga tgcggcgctg cattctgttc ctgaggcgcg
    11521 gcgtcaggcg tattttgcgc aaagatgagt ccggcggcag ccgcgtacag catcaaacca
    11581 taagcggcag cgcggatggg acaaaacgaa acgcttctca tagtcattcc tcgcggtgtt
    11641 cgacgcattt ttccctgcat ctgtttcgcc aggcacgact gtgagctaaa cacttgaaaa
    11701 accaaggcct gaatgctccg caaatggccg gaatccacca accctggtgc ttttcagcca
    11761 gcagcacggc tcccggccaa gcaccggatg gcgccgcgat accccgtaat tggccgtcgg
    11821 gcgccggcaa gagaagacgc cgcattgcga tctgagcttg agagtaaccg gctcgcaaat
    11881 tacgcgctgg ctgcgcagcg gaagccgacg tggccggcgg gatcgttcac ccgatgactg
    11941 tcccgcgaag tgacgcgcat gttgaacgcg ccgccctcga agctgccgcc gcggatgacc
    12001 cggcgctcgg ctttcgcgcc acgcattcgg cgcaggtctg ccagcgattg aatcactgca
    12061 tgggaaggat aagggctccg ccagccgtcc aggcagaact cccacacatt tcccgcgaga
    12121 tcaaacaggc cgtacggatt gggcgggtaa ctgcctacgc gaacaggccc atttttagcc
    12181 gattctgcgt agttggcgaa gcgcggggag gcagcctcgt cgccccaggg atacgtcgcg
    12241 tcaggacggc ccccgcgcgc tgcgaactcc cattctgctt cggttggcag acgcttgccc
    12301 gtctattcgg cgtacgccag cgccgcctgc caggttacaa agaccaccgg aaaatccgat
    12361 tggcccggtg gaaaccgatc gccgttccag gtcagaaggt agttgccgcc cacacgctct
    12421 tttctccact caggcctggc cgttacaaac gtgcggaact ccgaattagt gacttcgtag
    12481 cggtcgatcc aaaagggagg aatcttcacc tcatggaccg gcgtttcggc cagcagcatc
    12541 tcccgcaggc ccgcacgcgc actcgggaac tgctcgaaca agtacttctc atccgatccc
    12601 attcggaaga cgccccccgg gagatggacc atcccggtat gccgggatgc gagcgccgca
    12661 aatagcaagc ttcgccgggg aatcaacacc ccgttggacg ctgcacagaa ccggcggtta
    12721 gccggcgcag cgaagatttc cgccgcccgt aaacaaatgg cacggagttg actgaacgca
    12781 gccgtctcgg atcgagaaag accggcggat tgaccgcgaa gagcggcctt ctcgaatcag
    12841 cgcgctgtct gttgctcccc tggcccggcg cgaaaaatcc ggtgcgccgc tcatggggaa
    12901 ccaaaactcc ggggtcaaac cggcgcaagc cggaactcaa gcagccgcgc ggcacaggcg
    12961 tttccgtcct gtaacccttt gccgccggaa agcatcgttg tcgaaagagg agtctcgaag
    13021 gcaagaatta tgcgtttgcc agccggatcg tccgtacaca ggagacccaa cccgtttgcc
    13081 ggttctggcg ccgaggtttc aagacggcgc tttttgctct cgctcggtgc gctgcttccc
    13141 gccggactgc ggtcgcagtc cgccgctcag caagccccgc aggaacagga aggccgtccc
    13201 gtagccacat tcgggagcga cgtcaaggtt gtgaacctgt tcgtgaccgt gcgaaataaa
    13261 caggggcaga tcgtccgtga tctgaaaaag gaagatttca ttctggagga agatgggcgg
    13321 ccgcagacga tccgttactt ctcgcaagaa tccgatcttc cgctgacact cgggctggtc
    13381 gtggatacaa gcctgagcca gcggcgcgtg cttggcgggg agcgcaacgc gtgctacgta
    13441 ttcctggatg aaatgctgcg ggacaaggac atggctttcg tgattcattt cgaccgggaa
    13501 gtagagctgt tgcaagacct gacctcgtcc cgcaagcaac tggaagccgc tctggaacgc
    13561 ctggaaacgc cgcaactgag ccgccggccg caatggggag gacgcggcag gccgggctct
    13621 cccggtccct cgggaacgcc gggacggcgg ggcgtgggca caactcttta tgacgcggtt
    13681 ttcctggcat gcgatgagct gatgaaaaag cagagcgggc ggaaagcttt aatcctcctc
    13741 tcggacggcg tggacatggg aagcaaagtc agcctgccgg aagcgatcga gtccgcccag
    13801 cgggccgaca cgctcgtgta ttccattctt ttttcggatc agcaggccta tggatcgccg
    13861 ggcggtttcg gaagccgcgg cggttggggc ggaccgggaa tgggccgccg cggcagaatg
    13921 ggcggcgggc gagggtttcc ccaaccgcag gcgcggcctg acggaaaaaa gattctggaa
    13981 cggatttcga aagagacggg cggcgcgtta ttcgaggtct cgaagaaaga atcgatcggg
    14041 caaatcttcg atcgaatcca ggaagagctt cgcaatcaat acagcttggg ttacagcccc
    14101 gactccaacg cgggcgcggg gtatcggaaa atccacctca caacccggca aaaaaacctc
    14161 atcgtgcaga cgcgagacgg ctattacgcc ggagcgtaag ggcggaacgc cccaacgact
    14221 tactttcttg cttgcgccac agagtcccgg ctttttcatc gaccggccga cgcgcaatct
    14281 gcgtggccgc ggggcgggcg ctggcaagct cgtgggccac tgcaaatgcc atctcgcttg
    14341 gtgagtcgct gagcgtccag ccgttgtcct tcgggtgatg aagatcaacg gggtagctcg
    14401 caaccgcgat agcatcctcg aatttactgc aactcaagat gtcgttacgg gtcatgacgg
    14461 acttgctgac gatgcggccg catgctcgaa gacgtcgata aatgcaagcg caagggaatt
    14521 ctgatcaaca ccttcctgct cgccagtgac tacggcctag tgcaatttct ccagaaggtg
    14581 accgaaatct gccccggcgg caaggcctat ttcaccactc gctacaacct cggccaatat
    14641 ctcccgatga attacaggga ccgcaaaatg cgcgcaatcc attagccgct atgcgcggtt
    14701 gcgtctcgag cgagcgggtc atttgcaaat caaaaataaa gcttcagcgc caactgtacc
    14761 tggcgcggcg aattggcctg ggaagtcact ttgccgaatg ccgaggagcc aaccgtcaga
    14821 ttcggcggtg aaaactgcgg gtggttgagg gcgttgaagg cttcggcgcg aaactctacc
    14881 atcgagcctt catgcagccg cggcagcgca aactgcttaa acatcgacag ggccgaagtg
    14941 gacgttccag gcgcacgcgc cgtcgaaatc tcgcgcggag cattgcccac cgtgaatggc
    15001 gccggcgtaa ccgcattctg agggttcgcg aagtactgcg tgagccagtt ggagccgttg
    15061 ttacgggtca ggggacccag tagattcggt cgctgcgggc cgtacgtggg caaagcgcgg
    15121 gaggtgctca cgctaagcgc cagcggcatg ccgttatcga atcgccacag gccgttagtt
    15181 tgccatccgc ccagcgcggc attgacgaag ctgttccagc ggccgcccca gtgctttcct
    15241 ctgccgaacg gaagttgata gacatacgag aactgcagca cctgcggaat gtcaaattgc
    15301 gagacggaac gctccaggaa ccgcttgttg ggatcttgca agctggtcgc tcccccgagc
    15361 catgtcgtgc agctacaggc taccgaagaa tcatcgatcg atttggagaa aacataagtg
    15421 gccagcaact gcagcccgct tgaaaagcgc ttctccagcc gcacctggag agaatggtag
    15481 atggaatcgg cggacggcgg atcgttgccg ctgaagccgc tgaattgggg gtacggcttc
    15541 agcagttgag attgctgcac cgtcgccgcc gacaacccgc tggccggatt ggtaatgatt
    15601 ccgtaaaacg gattggcaac aaaggaattg agctgtgtga tctggtccgg agttgctgac
    15661 tccacccagg aacccagata atttaagctg cctgctcccc cgaaatagag gtgtgtgccc
    15721 ttggtgccga ggtagtttac gtccaacagc atgttgcctt tgagtttccg ctgcaaccca
    15781 aagttccatg tctgcatgta cggcgtgttg ttccaggt
//
LOCUS       DASREK010000229         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1269366, whole genome shotgun sequence.
ACCESSION   DASREK010000229 DASREK010000000
VERSION     DASREK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269366"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..2227
                     /locus_tag="VFA54_11975"
     CDS             278..2227
                     /locus_tag="VFA54_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HZS51574.1"
                     /translation="MPSQAPPAAGQTRASRARQFFGPGGLLAARHPEFEFRPGQLKMA
                     EEIEAALAEKRHLLVEAGTGTGKTLAYLVPVVASGRRVVISTYTKNLQEQLYYKDIPF
                     LEKHFDRSLHVAYMKGRNNFLCRQKLYDAQNKPLLSGLMEIEDFALIRQWESTTATGD
                     RAELVKLPETSTVWAKLDARRELCTGQKCPQFERCFISLMHQRAAESDIIIVNHHLFF
                     ADLAIRDLDFGSIIPDYSAVVFDEAHEIEDVAGQHFGVALSTHKFEELARDIESVARV
                     NDLASSGLHRTLGLLREGARDLFALLPGGEGRSGFRDRPCFLEKHQKQYESLLDAIDL
                     TASSLKLMQKKTEEIAPLLRRAAEVRLSLEFLLAGNDRSFVYWIERRNRGVFLQATPI
                     DVSQLLAERLFDPVDTVILTSATMAVEDKFDFIRKRLGITTARELIVPGHFDYRKQAL
                     LYVPRSLPDPRDPGFVRAAAGEVTRILKQSRGRAFVLFTSYQQMRQVHDLVSFAIEYP
                     TLLQGTAPKTALLEEFRSTPHCVLFATSSFWQGVDVPGEQLSCVIIDRLPFAVPSDPV
                     VEARIRNIREDGGNPFYEYQVPEAVLALKQGFGRLIRNRKDRGVLALLDNRILRQRYG
                     RIFFTSLPDYAHTTEFGEVEKFFQE"
     gene            2400..>2485
                     /locus_tag="VFA54_11980"
     CDS             2400..>2485
                     /locus_tag="VFA54_11980"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZS51575.1"
                     /translation="MFEISVERSFAAGHALRGYKGKCENIHG"
BASE COUNT          480 a          776 c          728 g          501 t
ORIGIN      
        1 gggcagatta ggccgggttg catcgcaaaa cttgaagagt tcaatcttag cacgcgaggg
       61 ggagattcac gagtcccgcc gcggccggac cgcggagaaa gaggcgagac cgccggcccg
      121 atctcggccg ctcgccggca gcctcttccg gagctcctcg aatgccgccc gcgccaggag
      181 cgggttcgcg atttcatctt tccgaagcta cccgtcattt ggagcgcgcc cgggcctcgc
      241 cgcgtccatc gcgatgttac gatagtcgct gccagccatg ccgtcacagg ccccacccgc
      301 cgccggccag acccgcgcgt ccagagccag gcagttcttt ggacctggcg gcctgctggc
      361 cgccaggcat cccgagtttg aatttcggcc gggccagttg aaaatggcgg aagagatcga
      421 agctgctctg gccgagaagc ggcatttgct ggtggaagcg gggacaggca ctggcaagac
      481 gctcgcctat ctggttccag ttgtcgccag cgggcgccgg gtcgtgattt ccacctacac
      541 caaaaacctc caggagcagc tttactacaa agacataccc tttttggaaa agcacttcga
      601 tcggtctctg cacgtcgcct acatgaaagg ccgcaacaat ttcctctgcc ggcagaaact
      661 ttatgacgcg caaaacaaac ccctgctcag cggcttgatg gagattgaag atttcgccct
      721 gatccggcaa tgggagtcca cgacggccac cggcgaccgc gcggaactcg tgaaattgcc
      781 tgaaaccagc acagtttggg ccaagcttga cgcccggcgc gagctctgta caggccagaa
      841 gtgcccgcag ttcgagcgct gctttatttc tctgatgcat cagcgggctg cggagagcga
      901 cattatcatc gtcaatcacc atcttttttt tgcggacctg gcgatccgcg acctcgattt
      961 cggctccatc atccccgatt acagcgccgt cgtgtttgac gaagcccatg agatcgagga
     1021 tgtcgccggg cagcatttcg gcgtggcgct cagcacccat aagttcgagg aactggcccg
     1081 cgacatcgaa tcggtggcgc gcgtgaacga tctggcctcg agcggcctgc accggacatt
     1141 gggcctgctg cgcgaggggg ctcgcgatct attcgctttg ctgcccggcg gcgagggccg
     1201 ctccggcttt cgcgaccgcc cctgctttct cgagaagcac caaaagcagt acgagtctct
     1261 gctcgacgcc atcgatctca cggcctcatc cctgaaactg atgcagaaaa aaacggaaga
     1321 gatcgcgcct ttgttgcgcc gcgcggcgga ggtacgcctc agtctggaat ttctcctcgc
     1381 cggaaacgat cgctcgttcg tttattggat cgagcgccgc aatcgcggcg tgttcttgca
     1441 ggccacgccc atcgatgtct ctcagttgct cgcagaacgc ctgttcgatc cggttgacac
     1501 tgtgatactg acttcagcga cgatggccgt agaggataaa tttgatttca tacggaaacg
     1561 cctgggcatc acaacggccc gcgagctcat agttccgggc cactttgatt accgcaaaca
     1621 agcgcttctt tacgtgcctc gctcgctgcc cgatccgcgg gatccgggat tcgttcgggc
     1681 tgcggccggc gaggtcaccc gcatcctcaa gcaaagccgc ggccgcgctt ttgtgctgtt
     1741 cacaagctac cagcagatgc gccaggtgca cgatttggta tcgttcgcca tcgagtaccc
     1801 cactcttctc caggggaccg cgcctaaaac ggccctgctc gaggagttcc ggtccacgcc
     1861 ccattgtgtg ctgtttgcaa cttccagttt ctggcaggga gtcgatgtgc ccggcgaaca
     1921 gcttagctgc gtcatcatcg accgcctgcc atttgccgtg ccgagcgacc ctgtcgtgga
     1981 agcccgtata cggaacatcc gcgaagacgg cggcaacccg ttttacgagt accaggtgcc
     2041 cgaggcggtc cttgcgctca agcagggttt cggccggctg atccggaacc gcaaggaccg
     2101 cggcgtgctt gcgctgctgg ataaccgcat tttgcggcag cgctacggcc ggattttttt
     2161 caccagcctg cccgattacg cccataccac ggaattcggg gaagtcgaaa agttcttcca
     2221 ggaatgaatg ctcgagcaag ctccgcccgg ggaccgggcg acgcccggct ggtctcgccg
     2281 cgccttccat cgccggaaaa agtgaaatgc ccctaatgcg ctatgattga tgtgcgggaa
     2341 caaaatcagc ctggccgctc ctgtcgccca ggacgccggg cttctccggc gcttccgtca
     2401 tgtttgaaat ttcggtggaa agaagctttg cagccgggca tgctctgcgc gggtacaagg
     2461 gcaagtgtga gaatattcac ggaca
//
LOCUS       DASREK010000230        22443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1929389, whole genome shotgun sequence.
ACCESSION   DASREK010000230 DASREK010000000
VERSION     DASREK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 22443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22443
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1929389"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFA54_11985"
     CDS             <1..508
                     /locus_tag="VFA54_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HZS51576.1"
                     /translation="LLRLTCGLEPGEEFDARLQQAFETIGFAKVSTSAEDARGLGFLR
                     LGDAVSMNRDRLVAQAKTAALALAENYQPAAPRNGIPAAGEAGYALLDLGIYLAHRAG
                     QISDYDAVVARKLAYVLSGGRLQGRPLVSEQYLLDLEREAFLSLCGQRQTQERMQHML
                     KTGKPLRN"
     gene            659..1834
                     /locus_tag="VFA54_11990"
     CDS             659..1834
                     /locus_tag="VFA54_11990"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZS51577.1"
                     /translation="MREAVIVSALRTAVGKAPRGTLRNTRPDDMAATVIQELLQRTPQ
                     IKPEDVDDVILGCAMPEAEQGSNVARIAAFRAGLPDTVSAMTINRFCSSGLQAIALAA
                     ERIRGYGAEIIIAGGAESMSLIPRGGHKPAPNPWLVERHPEVYLSMGLTAEALVEKYR
                     VAREDQDRFALRSHQNALAAQAAGKFDEEIVPLRVEYSTLDNGRPCRHELVFQKDEGP
                     RPDTTLEALAKLKPVFHVRGTVTAGNSSQTSDGAAAALVLSSDKARELGLKPLARFVS
                     FATAGVPPEIMGIGPVVAIPKALALAGLRLDDMDLIELNEAFAGQALAVIREAGLPLE
                     KVNVNGGAVALGHPLGATGAKLTATILHEMKRRGSRYGMVTMCIGGGQGAAGIFERI"
     gene            1936..3738
                     /locus_tag="VFA54_11995"
     CDS             1936..3738
                     /locus_tag="VFA54_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZS51578.1"
                     /translation="MATIATAASRAKGGSFLIESVSPDQIFTPEDFTEEQRQIAATTD
                     EFVNNEILPQVEALEHQEPGLTRSLLRKAGELGLLAVGVPEKFGGLEMDNVTALLVAE
                     HIAKYASFSSTFGAHSTIGTLPIVYFGTEEQKRKYLPKLASGEWMGAYCLSEPHAGSD
                     ALAARTRADLDPTGKVYLLNGEKMWITNGNWADLFTVFAKVGGEKFTAFLVERSFPGV
                     RSGPEEKKMGIKGSSTCPVILENAQVPAENLLGEIGRGHIIAFNILNLGRLKLGAGCV
                     GGAKRVLSISIRYAKDRKAFGKPICEFGLIQHKLAEMAIRTYVAETLVYRTGGLIDAQ
                     LAEFSWEQPGAEKTALKAVEEFAVECSVVKVYASEILDYIVDEGVQIHGGYGYHQDYA
                     VERAYRDSRINRIFEGTNEINRLLISGMLLKRAVKGQLELMPAAQALMNEVLSGSSLS
                     EPPGDGSLAAEKQIAANAKKIALLSAGAAFQRFLTDLENQQEVLAGISDMIMEAYAME
                     SAVLRAEKIINGGAAGVSGENVSDMAVVFVRDAMNRVESAARAVLAASAEGDALRAQL
                     AILRRLARFEPVNSIALRQRIARRLIAAEKYALS"
     gene            3813..4313
                     /locus_tag="VFA54_12000"
     CDS             3813..4313
                     /locus_tag="VFA54_12000"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS51579.1"
                     /translation="MLAVGLPGSGKSTYFARRGIVPLSSDWLRLILADDVTEQRFQPF
                     IFVALRFLVRMRLKLDRPVTYVDATNLTPAERGRYIAIGRRHGCQMEAIFFDAPLEVC
                     RERNRARKRRVPEEAMLALSARLQPPVLAEGFDKITTIYADGRTVTETVSGSQDAPSG
                     SERPGS"
     gene            complement(4310..5680)
                     /locus_tag="VFA54_12005"
     CDS             complement(4310..5680)
                     /locus_tag="VFA54_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS51580.1"
                     /translation="MKKNWILVVDDEPGIRESLSGVLEDEGYAVRAVGSGEEALAQLA
                     ARTYPVVLLDIWLPGLDGLGVLTQIQDLDAARRPAVVVISGHGTIETAVRATKLGAFD
                     FLEKPLSIEKVTVTVKNALERRRLLLENERLKEEVKSRYHIIGESVPMKALRQQLALM
                     APTNGRVLIYGESGTGKELVAHAIHAQSTRAEEAFVEVNCAAIPEELIESELFGHVKG
                     SFTGAHDDKIGKFQKADGGTLFLDEVGDMSLKTQAKVLRALEEQRFEPVGAAGSVQVD
                     VRVIAATNKNLEEEIDRGNFREDLFYRLNVVPFYVPPLRERKEDIPLLVNYFLSEFTT
                     AYGRKPKELTPAAYAALEEYPWPGNVRELKNLIERIVIMNPQVRVDVRHLPLPAARRP
                     AEIDRRSLPQFASLQEARNAYEREYILKKLEETNGNVTRTAEILGLERSNLYRKMKAL
                     GIGIKD"
     gene            complement(5885..8131)
                     /locus_tag="VFA54_12010"
     CDS             complement(5885..8131)
                     /locus_tag="VFA54_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS51581.1"
                     /translation="MRASHTKKSKPVVERKTARQRWYLLSLSLLLLALMTIVFWQTSL
                     SFGEFRPSDVQQTVVLWGISTLVFLGMITLGFMLFRNLVKLYLERRGNRLGSRIKTRL
                     VAGALGLTILPVVFLFFFSFNVLNRTLDKWFNQPIEQLQKDSKAISERLKDAARDKAE
                     TLAEWIASMPEVTAALEGERSVAALNRTLRDFALAHHLSYVAVGPSAQPPLIEHAESS
                     DLTGMWHRLPASALRLGAAGSAVFSDQGDEIVYAYRPVRSLGWVVAGWQMPEDLAAKQ
                     QAVDARYRSYQEQAKYWRFMRYFYVGMLSLITLFILFVAAWIALFLSKQIVAPIERLV
                     QATAQLSSGRLDYRIETRAIDELGLLVRSFNQMTEQLEHSRDELEARRRYTEAILESI
                     PTGVISLASSGEILRVNPALGKIFTPEKAAPARWLEDLFAAEDAAKVQRLIRRAQRTG
                     LATGSLDLTSDGQTKHLAITVSALEQDGRPEQSGVPRGCVIVLEDMSELLRAQKSAAW
                     QEVAQRVAHEIKNPLTPIALSAERISRLVARFGPSQDVRGRLELERVLHGCTATVNRE
                     IETLKTLVNEFSQFARFPAAHPEPGDLNEVVEAALDTYNGRLHDLRVVKQLAESLPPV
                     AIDHEQLRRAVVNLIDNALEAMDGCRDNGEEQQIVISTGAGAGGDMVELTIADSGHGI
                     QPEDREKLFLPYFSTRKRGTGLGLAIVSRIIAEHKGTIRVEDNKPRGAKFIVEIPVIE
                     AKVAAKVS"
     gene            complement(8103..8837)
                     /locus_tag="VFA54_12015"
     CDS             complement(8103..8837)
                     /locus_tag="VFA54_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51582.1"
                     /translation="MRQRAGNARKDGHSKKPAPLVSGCPAAAEGILRRFYEALLAHFG
                     PQDWWPGETRFEIVVGAILTQNTAWSNVEKAIANLKRAGVLAVEAMEALPESDLAELI
                     RPSGYYRQKAKKLKAFLSYLRRKHGGSLERMFRTPTAQLRLELLAIYGIGEETADSIL
                     LYGGDHPIFVVDAYTRRILERHRAAQPSWSYRQIQALFHSEIRPDAAVYNGFHALLVV
                     TGKQHCWKRRPRCEGCPLEGFPHQKE"
     gene            complement(8873..9676)
                     /locus_tag="VFA54_12020"
     CDS             complement(8873..9676)
                     /locus_tag="VFA54_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51583.1"
                     /translation="MRFIAVPVMLFAACGWVLGQTGEPSQGLREPVKNVESQPENGIY
                     VVKSGTRVPLALINSVSTKHSSPGDRIYLESVFPILVNGKIVIPPGSYFEGTVTQVKR
                     PGRVKGRGAIFIRFDSMILPNGVTRDFRARVGGLDGRSSEDLDRKEGKITSEGNKAGD
                     ARTVGEAAAGGATVGAIAGSAAGSPGMGVGIGAAAGAAAGLAGVLLSRGPDAVLARGT
                     QLDMVLDRDLTFNASEINFPNGAQPIPIEGGPVSPRDRAAPPVGRRFPY"
     gene            complement(9911..10702)
                     /locus_tag="VFA54_12025"
     CDS             complement(9911..10702)
                     /locus_tag="VFA54_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51584.1"
                     /translation="MRPGWWYLLATAAAAAVLTAADGVPSPEAPLINVGGVVNAASSI
                     PAPNNFVAPGTIISIYGTGLAKTTREVRASDLDNGFLPVTLDGVSVFFGPVAAPLFYI
                     SPLQINAQVPFELQPGEWQVTVRVDSLQSSGSVVVRPYSPGLFNAFRHNDGTLVSKDA
                     PAKPGEFILFFGTGFGPLRQPAPLTGQLAPSGQSWMVNPVQAAIGGEALAPEDIYYWG
                     LAPGYAGLYQFNLRVPWDAPEGSLEVLVGIEGQWSQKGILLPVRH"
     gene            complement(10852..12273)
                     /locus_tag="VFA54_12030"
     CDS             complement(10852..12273)
                     /locus_tag="VFA54_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor V domain-containing protein"
                     /protein_id="HZS51585.1"
                     /translation="MNTEISKRSGRCALVLGLCGALLPALWSYSEGPDPFKTGGPFPG
                     ESSCAESGCHSSPSRLNKGPGMVSISVNPYKPGETQTITVTVSDPTEQRWGFQLTARP
                     ANSLMTSAGSFQPLDGNVQVVCGDPKNPSFGPVPCGAGTISFAEHTLPGTRLGTRGGV
                     TFQVNWTAPSSDVGDIIFAAAGNAANGNSQADPGDNIYTTSVTVSAAGSGAGPPPEIS
                     SGGIVGAGLSNPPVAQISPNGLISIFGQNFAAPGVLRGVTAGDLVDGHLPSNLAGVCV
                     QIDSRPAPMFFVSPTQINAQAPSLSSTGAVGVQVIQNCGASRQVISNTQTVVMQPVAP
                     EFFFFVHNSGGPSPVAATNAITGSLIGAPGLLPGAVFAPAKPGDFVALYGTGFGATNP
                     SFAAGVLPDAIGATVSKVTVTLNNVVLADSDVLYAGVAPGFAGLYQINIHIPANMPDG
                     DLPISASINGVSTPPGAFITVQR"
     gene            12516..14948
                     /locus_tag="VFA54_12035"
     CDS             12516..14948
                     /locus_tag="VFA54_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZS51586.1"
                     /translation="MPLLLTVLQGPGGKSEKDPICGMQVDPLHAAGRHEHKGRVYYFC
                     SRSCLEKFRAEPEKYLKPAPAAPPIARPPAAKAPAEYACPMHPEVVRAGPEACPACGM
                     ALEPRTPLRVEYVCPMHPQIVRSAPGTCPICGMALEPRTTLLAEESNPELVSMTRRFW
                     VSAVLTAPLLLSMIFGHLPALAQLALATPVVVWGAWPFFERAWASLVNRSLNMFTLIG
                     MGTGAAYLYSLIAALWPGIFPEAFRSHTGRPEVYFEAAAAITTLVLLGQVMELRARGR
                     TSAAIRSLLNLTPKTARLVQPNGDEQDVPLDHIRPGDRLRVRPGERVPVDGRILEGSS
                     SIDESMLTGEPIPVEKGPGDRVTGGAVNGSGSFIMQAERVGAETILAQIVRQVSEAQR
                     SRAPIQRLADKVSQVFVPAVVLAAAITFVVWSQAGPQPRLAHALVNAVAVLIIACPCA
                     LGLATPMSIMVGIGRGATSGVLIRSAEALEIFEKVDTLVVDKTGTLTQGKPHLKAAIA
                     FQEGKFLEEDSQSAAEILRLAASLEKASEHPLAGAIVSEAQKRGLPLASPSDFQMIPG
                     KGVTGTVEGRRVALGSFPLMEDLGAGAEPLFERARGFQSDGATVLLAAIDGRPAGLLA
                     VEDPIKESTREALAALHREKIRVVMVTGDDPATAKAVARKIGIDEVEAGVLPDRKGEI
                     VKQLQAEGRIVAMAGDGINDAPALAQADVGIAMGTGADVAIESAGITLVKGDLRGIAR
                     ARRLSAATMRNIRQNLFFAFVYNLLGVPIAAGVLYPAFGLLLNPIIASAAMSFSSVSV
                     VGNALRLRHISL"
     gene            complement(15089..16048)
                     /locus_tag="VFA54_12040"
     CDS             complement(15089..16048)
                     /locus_tag="VFA54_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZS51587.1"
                     /translation="MLQQTRAAAAVPYYERFLRRFPDLNSLAQSSETELLRYWSGLGY
                     YSRARNLRRAARYIVSRMGGAFSSRYADWKELPGVGPYTAAAVASIAFGEPVAVLDGN
                     VARVLARMTNHSGDIRSARVREALRRRAQAWLEATRSGGKGGSHTPETANTAGNFNQA
                     LMELGATICVAQNPRCSLCPVAAHCQALLLGTQNGLPVRLESRRPVRLRIAVAVVERG
                     RKLLLRQRPPHSSQMPGFWELPQAENEQPDPDCLASLGLTLKERLREFRHSITYHNYT
                     ITVFRAALRGPTPQGFRWRAVEELPEIPLTTITKKALKTIAAV"
     gene            complement(16382..17086)
                     /locus_tag="VFA54_12045"
     CDS             complement(16382..17086)
                     /locus_tag="VFA54_12045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZS51588.1"
                     /translation="MKTRLLILLASAALAWAQEQPKTAPAAPAEREPGLYATINTSMG
                     PIVCKLFEKEAPLAVANFVGLARGTKEWTDPKTGQKVKRPLYNGTIFHRVIPGFMIQG
                     GDPLGTGMGDPGYKFKDEFVASLKFDQPGRLAMANSGPNTNGSQFFITEVPTPHLNNH
                     HTIFGQVLEGQELVTKIANVPRDSNDKPRTPVVIRSIRIQRYPLAAAAKGAAPPGKTG
                     TAKKTAPAANATPAKK"
     gene            complement(17251..18243)
                     /locus_tag="VFA54_12050"
     CDS             complement(17251..18243)
                     /locus_tag="VFA54_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZS51589.1"
                     /translation="MTASRSVLVIGGTQFIGRELVRRLLARGDRVAILHRGRSVIPAG
                     ASEILCDRNDTGAIRRALADRGFDLVFDNVYDAERGTTAAQVQAAAEACAGHVRRYVF
                     MSSVAVYPEGRDWEEGARLVSAGQSSEYALNKAESEQMLFSMHREHGFPAVTLRPPFI
                     YGPENPYYRESFFWDRIVRGRPVLIPEDGARLMQFVYVKDLVWAALQAAEAPASPGQA
                     YNIAHAPPVTQVEAVQALAAAAGLPARLVFVHRRRIAAAGGNVFQPPLYFGEYLDLPS
                     ITVSIAQARRELGFEPTAFVAGLRETFQWYLAQKRPEPDFSWEDRLLGTLHQEL"
     gene            18353..19159
                     /locus_tag="VFA54_12055"
     CDS             18353..19159
                     /locus_tag="VFA54_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HZS51590.1"
                     /translation="MSHTAEKQISAPSLSTLVVDDEQLAREELCYLLRECPGIDLVGA
                     GTNGLEALQLIHAHEPDLVLLDVQMPGLDGMGVVRRLQNQGGVLPHIIFTTAYDQYAV
                     EAFEVNAVDYLLKPIDKSRLQVSLDRVRRLVESRLGAGARIEAEAVPQAASRPGARTK
                     LLVKQAGRLFLVDSEDVVYATIDDGLITIVATMIEGQSNYRTIEELQSSLDPASFWRA
                     HRSYLVNINKIKEVVPWFKSSYQLRMDDKKHTEIPVSRAQTRRLRELLKL"
     gene            19168..19407
                     /locus_tag="VFA54_12060"
     CDS             19168..19407
                     /locus_tag="VFA54_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZS51591.1"
                     /translation="MTILPVEKEKLPESIRPIYQNMEQKTGKVLNFFKFMANKPDAMR
                     AFLDFYKAVWAPGALSPKIKELAYLATSVSNYCEY"
     gene            19444..19698
                     /locus_tag="VFA54_12065"
     CDS             19444..19698
                     /locus_tag="VFA54_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51592.1"
                     /translation="MTEEQIQALKDPGGVQREVFTAEERAAVRYARLLTKHPSGVRQE
                     DLDELGKHFNAEQIVELTLVIGAANLTNRFNDGLRVPVDV"
     gene            complement(19730..20842)
                     /locus_tag="VFA54_12070"
     CDS             complement(19730..20842)
                     /locus_tag="VFA54_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51593.1"
                     /translation="MWRKGALFPPEHAISRDRRTGAVVHQVTNHPSINHPSYFLHSSF
                     TPDQKALLFTSYRSGAPQLFEAAFPAGGIRQLTDGPPIHPFSPAIAPDGSRVFFVRGG
                     SVWAVDRATLEEQLIFELSGAQLGECSLSPDGCWITAACKRGGSAGIAVGRADGNAWR
                     VIPFPRTVIHPQFSPVEPEWIEFAGDPAPRMHRVRRDGSGLECLYENTNDEFVVHETF
                     LGLTGDLVFAVWPRALKRMNWRTRAIETIAECNAWHIAADRAGTVVLCDTNHPDAGVQ
                     LIRVASGERKTLCYPEASNQGSQWRKSRYALEEDWAAARSEKENALSWMEMSVDTVYG
                     PQWTHPHPSFSPDEKLVAFASDRTGFTQVYVVERDE"
     gene            20890..21228
                     /locus_tag="VFA54_12075"
     CDS             20890..21228
                     /locus_tag="VFA54_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51594.1"
                     /translation="MPRYRIHYLKEPQRLHFRQAGPSPAPARLKKKDYEAGGEIEATS
                     PYAAWKKMRADGAEHPIDVGDALETEAGTLLLCKYVGFEEAQWMAPPSESAGNDDTAS
                     LAHAPIETPL"
     gene            complement(21262..22164)
                     /locus_tag="VFA54_12080"
     CDS             complement(21262..22164)
                     /locus_tag="VFA54_12080"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS51595.1"
                     /translation="MDFDQIATFVEVAHAGSFSRAAQRLYRTQPAVSTQVRLLEKQCG
                     QALFVRHARQVELTVAGKTLLRYGERLRELRDAALAELDLMRRRPRGTVAIAANEATV
                     LYVLPAAILQFKRDYPDVRLEITRNFSRKIIEKVLENTVDFGVVTLPFKHAHLNVLPI
                     HRDEMAVLTPPGHPLARRESVKLEEVVEYPLLYPKTGQTRLQLDRHLRRFQDRLKISM
                     ELASVETIKKFVASGIGVTLMSSAFAQAEQAAGLLKVVPLEEKIYRDLGLIYHRHKYI
                     CPVAEAFLSTMRRCFGIGGTGARA"
     gene            22210..>22443
                     /locus_tag="VFA54_12085"
     CDS             22210..>22443
                     /locus_tag="VFA54_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS51596.1"
                     /translation="MFHNRSVAAMDFDQLRSFVEVARLKNFSRAAGKLGLTQPAISTQ
                     IRQLEDEFGVRLFDRIGKKVFLTQPGLLLLDHAG"
BASE COUNT         4343 a         7024 c         6798 g         4278 t
ORIGIN      
        1 tctgctgcgc ctgacgtgcg ggcttgaacc aggggaggag ttcgacgccc gcctccagca
       61 ggcgtttgaa actatagggt tcgccaaagt gtcgaccagc gcggaggacg cacgcgggct
      121 cggatttttg cggcttggag acgccgtttc tatgaatcgc gaccggctgg ttgcgcaagc
      181 taagacggct gcgctggcgt tggctgaaaa ttatcagcct gccgcgccgc ggaacggcat
      241 cccggcagcc ggtgaagcgg gttatgccct gcttgatctc ggaatttacc tggcccatcg
      301 cgctgggcag atcagcgatt atgacgcggt cgtggcccgc aagctggctt atgtattgtc
      361 gggcggccgg ctgcaaggcc ggccgctggt ttcggaacaa tacctgttgg atttggagcg
      421 ggaggccttc ctgagcttgt gcggccagcg gcaaacgcag gaacggatgc agcatatgct
      481 caagaccgga aaaccgctga gaaactaggc ggcggttcct cgcgcccaag ccgccggctc
      541 aacctagagc aaggcggcgg gagcctgagg ctaccgctgg aagaagggac tgagcttggc
      601 ggccgcccgc gacatccggc tgttggaatc gcagggcggc gcggcaaagg gagtcttcat
      661 gcgagaagca gtcatagtaa gcgcgctgcg tacagccgtt gggaaggcgc cgcgcggcac
      721 gctgcgaaac acccgcccgg atgatatggc cgccacggtg atccaggagc tgttgcaacg
      781 gacaccgcag ataaaaccgg aggatgtgga cgatgtaatc ctgggctgcg caatgccgga
      841 agcggagcag ggatcgaatg tggcccgcat cgcagcgttt cgcgcgggcc ttccggacac
      901 cgtgagcgcc atgaccatca accggttttg cagctccggc ttgcaggcga tcgccctggc
      961 ggccgagcgc atacgaggct acggcgccga aatcatcatc gccggcggcg ctgaatccat
     1021 gagcctgata ccgcggggcg gccacaaacc agcgccaaat ccctggctgg tggagcggca
     1081 tcccgaggtt tatctctcca tggggctcac cgcggaagct ctcgtcgaga aatatcgcgt
     1141 ggcccgtgag gaccaggacc gcttcgcatt gcgcagccat cagaacgctc tggccgcgca
     1201 ggctgcgggc aagtttgacg aagaaatcgt gcctctgcgt gtggaatact cgaccctcga
     1261 caatggccgt ccctgccggc atgagctggt ctttcagaag gacgagggac cccgcccgga
     1321 taccactctc gaagcgcttg ccaagctaaa gccggtattt cacgtacggg gaacggtgac
     1381 cgccgggaat tcctcacaaa ccagcgacgg ggcggccgcg gctttggtgc tctcttcgga
     1441 taaggccaga gaactgggtt tgaagcccct ggcccgtttc gtcagtttcg caacggcggg
     1501 agtgccgccc gagatcatgg gcattgggcc ggttgtggcg atcccgaagg ctctggcttt
     1561 agccggatta cgcctggacg acatggatct gatcgagctg aacgaggcat ttgcgggcca
     1621 ggctctggcc gtaattcggg aggcgggcct gccgctggag aaagtaaacg tgaacggcgg
     1681 agcggtcgcg ctcgggcatc ctctcggagc cacgggcgca aagctcaccg cgacaatcct
     1741 gcacgaaatg aaacggcgcg gctcccgcta tggcatggtt acgatgtgta tcggcggcgg
     1801 ccagggcgca gcgggaattt tcgagagaat ctgaaaagct gttgcctctg ctccgcgccg
     1861 ccggcatctc ctggctggcc cgcgtactcc cgtcggggag tcatcgcaag ggaacttgca
     1921 aggaaggagt ttttgatggc taccatcgcg acagcggcgt cccgagcaaa gggcggcagc
     1981 ttcctgatcg aatctgtctc accggaccag attttcacgc cggaagactt caccgaggag
     2041 cagcgccaga ttgcggctac gacggatgaa ttcgtaaaca acgaaattct gccccaagtg
     2101 gaggcgctcg aacaccagga gcctggtttg acgcgttcgc tgctgcggaa agcaggggaa
     2161 ctgggtttgc tcgcggtcgg agttcccgag aaattcggcg ggctcgaaat ggataatgtc
     2221 acagccctcc ttgtcgccga gcacatcgcc aaatatgcgt cgttctccag cacgttcggc
     2281 gctcacagca ccatcggcac tctgccgatc gtttacttcg ggaccgaaga gcaaaagaga
     2341 aagtatctgc cgaaactggc cagcggcgag tggatgggcg cgtattgcct gagcgagcct
     2401 catgccggca gcgacgcctt ggccgctcgc acccgcgccg acctggaccc cacggggaag
     2461 gtctaccttc ttaacggcga gaagatgtgg atcaccaacg gcaactgggc ggacctgttc
     2521 acggtttttg ccaaagtcgg cggggagaaa ttcacggcct ttctggtgga gcgttctttt
     2581 ccgggcgtgc gctccggccc cgaagagaag aagatgggca tcaagggcag ctcgacctgc
     2641 cccgtaattc tggaaaacgc gcaagtgccg gccgaaaacc tcctcggaga gatcgggcga
     2701 ggccatatta tcgcgtttaa tatcctgaat ctgggccggc tgaagctggg cgccggatgc
     2761 gtgggcggag ccaagcgagt tctgtccatt tccatccggt acgccaagga ccgcaaggcg
     2821 ttcggcaaac cgatctgcga attcggcctc atccagcaca agctcgcgga aatggcgatt
     2881 cgaacgtacg tggccgagac gctcgtgtac cggacgggcg gcttgatcga cgcgcagctt
     2941 gctgagtttt cctgggagca gcccggcgcc gaaaaaacgg cgctgaaagc agtggaggaa
     3001 ttcgctgttg agtgctcggt cgtgaaggtg tacgcctctg aaatcttgga ctacatcgtg
     3061 gacgagggtg tgcagatcca cggcggctac ggctaccatc aggactacgc ggtggagcga
     3121 gcctatcgcg actcccggat caaccggatt ttcgagggca cgaacgagat caaccgcctg
     3181 ctgatctcgg gcatgctgct aaagcgtgcg gtgaagggac aactggagct gatgcctgcg
     3241 gcgcaggcgc tgatgaacga agtgctgtca ggctccagcc tctctgagcc gcccggcgat
     3301 ggatcgctgg cggccgaaaa gcagatcgct gccaacgcga aaaagatcgc tttgctttcg
     3361 gccggtgcag cgttccagcg gtttcttaca gatttggaaa accagcagga agtgctcgcc
     3421 ggcatcagcg acatgatcat ggaagcttat gccatggaat ccgcggttct gcgggcggaa
     3481 aagatcatca acggcggcgc tgcgggtgtc tccggcgaga acgtttccga tatggcggtc
     3541 gtcttcgttc gcgatgccat gaaccgcgtc gaaagtgcgg cccgagcggt gctggcagcc
     3601 tcggcggaag gcgatgcttt gcgcgcgcaa ctggcgattt tgcgccgcct ggcgcgcttc
     3661 gagccggtca actcgatcgc cctccggcag aggatcgccc gccgcctgat cgccgcggaa
     3721 aagtacgccc taagctgagc gccgtgccgg agcacgccag ggctgagaat ccggcggggc
     3781 catctccccg gcgcgcagcg cggcagagac tggtgcttgc ggtcgggttg cctggttccg
     3841 gcaaatccac ttacttcgcc cggcgcggca ttgtgccgct ctcgagcgac tggctccggc
     3901 tgattctcgc tgacgacgtt accgagcagc gcttccagcc gttcattttc gtcgcgcttc
     3961 gatttctcgt gcgcatgcgg ctcaaattgg atcgccctgt tacctacgtg gatgcgacga
     4021 atctcacgcc cgccgagcgc ggacgctaca ttgcgatcgg caggcgccat gggtgccaga
     4081 tggaagccat cttcttcgat gcgccgctcg aggtctgccg cgagcgaaac cgcgcccgca
     4141 agcgccgcgt tcccgaagag gccatgctgg cgctctccgc gcgccttcaa ccgccggttc
     4201 tggccgaagg ttttgacaag atcaccacaa tttacgccga tgggaggaca gttaccgaga
     4261 ccgtctcggg cagccaggat gccccttccg gctccgagcg gcccggatcc taatctttga
     4321 ttccgatccc gagcgccttc atcttgcggt acaggttgct gcgttcgagg cccaggatct
     4381 ccgccgtgcg cgtgacgttt ccgttggtct cctccagttt cttcagaatg tactcccgct
     4441 cataggcgtt gcgggcttct tgcaggctgg cgaactgcgg caaagagcgg cggtcgattt
     4501 cagccgggcg ccgggccgcc ggcaagggca aatgtctcac atcgacgcgc acctgcgggt
     4561 tcatgatgac gatccgctcg atcaggttct tcagctcccg gacgtttccc ggccatggat
     4621 actcctccag agccgcatag gccgcgggcg tgagctcctt gggtttccgg ccgtaagcgg
     4681 tcgtgaattc gctcaaaaaa taattgacca ggaggggaat gtcctccttc cgctcgcgca
     4741 ggggaggcac gtaaaagggc acgacattga ggcggtagaa aaggtcctcc cgaaaattac
     4801 cgcggtcgat ctcttcttcc aaattcttat tcgtcgcggc gatcaccctc acgtccacct
     4861 gcacggatcc cgcagcgcct actggttcga acctctgttc ttcgagcgcc cggaggacct
     4921 ttgcctgggt cttgagactc atatctccga cttcgtcgag gaacagcgtc cccccgtcag
     4981 ccttctggaa cttgccgatc ttgtcgtcat gagcgccggt gaacgatccc ttgacgtgcc
     5041 cgaacagttc gctctcaatc agttcctccg gaatcgctgc gcagttcacc tcgacaaacg
     5101 cctcttcggc gcgggtgctc tgggcgtgga tggcgtgcgc tacgagctct ttgccggtcc
     5161 cgctctctcc gtaaattaac acccggccgt ttgtgggggc catcaaagcc aactgctggc
     5221 gcagggcctt catcggaacg ctctcaccga tgatgtgata gcgggatttg acctcttcct
     5281 tcaggcgctc gttttccagc agcagccggc ggcgctccag ggcgtttttc accgtcaccg
     5341 tgactttctc gatcgacaac ggcttctcca gaaagtcgaa cgcgccgagt ttggtcgccc
     5401 gcaccgctgt ttcaatggtc ccgtggccgg atatgactac gacggctgga cgccgggccg
     5461 cgtccaaatc ctggatctgc gttagcacgc ccagtccgtc caagccgggc agccagatgt
     5521 ccaatagaac caccgggtaa gttcgggccg cgagctgcgc gagcgcctcc tcccccgagc
     5581 cgacagcgcg cacagcgtag ccctcgtcct cgagcactcc gctcagggac tcccgaatgc
     5641 caggctcgtc gtcaacgacc aaaatccagt tcttcttcat gagaccaggt tcccgtacca
     5701 cagacgcgcg gcagcgcccc cgcttcgacc cgctcaaccc ttccgcgatt cggcgccgcg
     5761 agcgagaccc tgattccgtg ccgggctttg ccgtttgtaa acccgccacg cggacccgtc
     5821 gccttcggac tcgcgggccc gtcctccggc gacagcccct cccgatcgcg ccagccgtcc
     5881 gctctcatga aactttcgcc gccaccttcg cctctataac cggaatctcg acgataaact
     5941 tcgctccgcg cggtttgtta tcttcaaccc ggattgtgcc cttatgctca gcgataatcc
     6001 ggctgacgat ggccagaccc aggcccgtgc cccgctttcg cgtggaaaaa taaggcagga
     6061 aaagcttctc gcgatcctcc ggctgaatgc catggcccga atcggctatc gtaagctcga
     6121 ccatgtcccc gccggcgccc gctcccgttg atatgacaat ctgctgctcc tcgccgttgt
     6181 cgcggcagcc gtccatagct tccagggcgt tgtcgatcaa attcaccacg gccctccgga
     6241 gctgctcgtg atcgatggcg acaggaggta atgattccgc gagctgcttt acgactcgca
     6301 aatcatgcaa ccttccgttg tatgtatcca gggccgcctc gaccacttca ttgaggtcgc
     6361 cgggctcagg gtgcgcggcc ggaaagcggg cgaattgcga aaactcattc accagcgtct
     6421 tgagcgtttc aatctcgcgg ttgacagtgg ccgtacagcc atgcagcacg cgctccagct
     6481 cgagccggcc gcgcacatcc tggctcggcc caaaccgcgc caccaggcgg cttatgcgtt
     6541 ccgccgagag tgcaatcggc gtcagaggat tcttgatctc gtgcgcgacg cgctgggcca
     6601 cttcctgcca ggccgccgat ttttgcgccc gcagcagctc gctcatatcc tccagcacga
     6661 tcacgcaccc gcgcggcacg cccgactgct ccggccggcc atcctgttcc aaagccgaaa
     6721 cagtgatcgc cagatgcttg gtttgcccgt ccgaagtcaa gtccagcgat ccggttgcaa
     6781 ggccggttcg ctgcgcgcgg cggatcagcc gctgcacctt ggcggcatct tcggcggcga
     6841 acaggtcttc cagccagcgc gccggggccg ccttctccgg cgtgaagatt tttccgagcg
     6901 ccgggttcac gcgcaatatt tcgccgctcg acgccagaga aatgacgccg gtcgggatgc
     6961 tttccagaat cgcctctgtg tagcgtcggc gggcttccag ttcgtcccgg ctgtgttcaa
     7021 gctgttcggt catctggttg aatgaacgca ccaacaagcc cagctcatcg atggcccggg
     7081 tttcaatccg gtagtcgaga cgcccgctcg aaagctgagc tgtcgcctgt accagccgtt
     7141 caattggggc gacgatttgc ttggagagaa acaaggcgat ccaggcggcg acgaacagaa
     7201 taaacagcgt gatcagcgaa agcatcccga cataaaagta ccgcatgaac cgccaatact
     7261 tagcctgctc ctggtaggac cggtaccggg cgtccaccgc ctgctgcttg gctgcgaggt
     7321 cttccggcat ctgccagcct gcaaccaccc atcctaagga tcgtacaggc cggtacgcat
     7381 aaacgatctc gtcgccctgg tcgctgaaga cagcgcttcc agcggctccc aggcgaagcg
     7441 ccgaagctgg gagcctgtgc cacattccag tcagatccga agactccgcg tgctcgatca
     7501 gcggcggctg cgcgctcggg ccgacggcga cgtaggaaag gtggtgcgcc agcgcaaagt
     7561 cccgcaaggt cctgttaaga gccgcgacgc tgcgttcgcc ttccagagca gcggtgacct
     7621 cgggcatcga cgcgatccat tcggccagcg tttcggcctt gtcccgcgcc gcatccttca
     7681 ggcgctcgct gatcgctttc gaatccttct ggagctgctc gatcggctga ttgaaccatt
     7741 tgtccagcgt gcgattcagg acgttgaagg agaagaaaaa caggaagacg accggcagaa
     7801 tcgtcaggcc gagcgcccca gccaccagcc tggtcttgat tctggatccc agccggttcc
     7861 cgcgccgctc gaggtaaagc ttcaccaggt tgcggaacag catgaagccg agggtgatca
     7921 tccccagaaa caccaaggtg gagatgcccc agagcacgac ggtctgctga acgtcgcttg
     7981 gacgaaactc gccgaacgac agggaagtct gccagaagac gatggtcatc aacgccagca
     8041 gcaggaggct caagctcagc aggtaccagc gttgtctggc ggttttgcgc tccacgacag
     8101 gcttactctt tttggtgtgg gaagccctca agcgggcacc cctcgcaccg aggccgcctc
     8161 ttccagcagt gctgcttgcc ggtcacgacc aacagcgcat ggaacccatt atatactgcg
     8221 gcgtccggac gaatttccga gtgaaacagc gcttgaatct ggcggtaaga ccacgatggc
     8281 tgggccgccc gatgccgctc gagaatgcgg cgggtgtagg catccaccac gaagatcgga
     8341 tgatcgccgc cataaagcag gatggaatcg gccgtctctt cgccgatgcc gtagatggcg
     8401 agcagctcca aacgcaattg cgcggtcggc gtccggaaca tccgctccag agaacccccg
     8461 tgcttgcggc gcagataact cagaaacgct ttcagcttct tcgccttttg ccggtagtag
     8521 ccggaagggc ggatcaattc cgccaaatcg gactcgggca gagcttccat ggcctcgacg
     8581 gccagcacgc cggctctttt caggttggca atggcctttt ctacgttgct ccaggccgtg
     8641 ttctgggtga gaatggcgcc tacgacgatt tcaaaacgtg tttcacccgg ccaccagtcc
     8701 tgcgggccga aatgcgccag caaagcttcg taaaaacggc ggagaatacc ctccgcagcg
     8761 gccgggcaac ccgaaacgag cggcgcaggc ttcttcgaat ggccatcttt tcttgcattt
     8821 cctgcgcgct gtctcactgc ttgtctgagc ttgacgccag agggactgtg tcttagtaag
     8881 ggaatcgccg gcccacggga ggcgcggctc tgtctcgcgg ggacaccggc ccgccctcga
     8941 tgggaatcgg ctgcgcgcca ttcggaaaat tgatctcaga ggcgttaaaa gtcaaatcgc
     9001 ggtcgagcac catatcgagc tgcgtgccgc gagccaacac ggcgtccgga ccgcgcgaca
     9061 agagcacccc tgccaggccc gcggcggctc ccgccgcagc gccgatgccc acgcccatcc
     9121 cgggacttcc ggcagccgaa cctgcgatcg ctcccactgt tgctccgccg gccgcagcct
     9181 cgcccacggt gcgcgcatct ccggccttat tgccttcgct cgtgattttg ccctccttgc
     9241 ggtccaggtc ttctgaggaa cggccatcca ggccccccac gcgcgcccga aaatcgcggg
     9301 ttactccatt cggcaagatc atcgagtcaa agcgaataaa gatcgcgccc cggcccttca
     9361 cccgccccgg ccgtttcact tgcgtgactg taccttcgaa gtaactgccg ggagggatca
     9421 caatcttgcc attcaccaga attgggaaaa ccgattccag ataaatgcgg tcgccaggcg
     9481 acgagtgctt tgtgctcacg ctgttgatca aagccagcgg aacacgggtg cccgacttca
     9541 ccacataaat tccgttctca ggctgcgact caacgttttt aaccggttcc cgtaaccctt
     9601 ggctgggttc cccggtctgg ccgaggaccc atccgcatgc cgcaaacaac attaccggga
     9661 cagcgatgaa cctcatccac agcgctcctc tctcaatttt agccgagctt ctcggcccgc
     9721 ccaacgaaaa tccactccga cagtagaaat gcgggaaaca ctgagggagt ttccaggaat
     9781 ttcccggcgc aggcgctgtt ttgcggtacg ttcgccgcct ccagacaagg agcttcttgc
     9841 gaatcaagca gctaccgcgc cacaacggcg ccccaaaagc gggcagaggt ccgaaatctc
     9901 ccgacgcctc ctaatggcgc accggaagca ggattccttt ctgcgaccac tgcccctcaa
     9961 tgcccacaag cacttccaga ctcccctcgg gagcgtccca tgggactcgc agattgaatt
    10021 gatacaaccc cgcataccca ggcgccagcc cccagtaata aatgtcctcc ggagcgaggg
    10081 cttccccgcc gatggcggct tgcaccgggt tgaccatcca ggactggccg gaaggggcta
    10141 gttgcccggt caggggagcc ggctggcgca gcgggccaaa tccggtgccg aagaacaaaa
    10201 tgaactctcc cggcttggct ggcgcatcct tactcaccag ggttccatca ttgtggcgaa
    10261 acgcattgaa aagtcctgga gagtacggcc gcacaaccac cgaaccgctc gattgcagag
    10321 agtccacccg cacggtcact tgccattcgc cgggctgtaa ctcgaacgga acctgggcat
    10381 tgatctgtag tggagagatg tagaagagag gagccgcaac cggaccgaag aagacggaga
    10441 ccccatccag cgtgaccggc aggaaaccgt tgtcgagatc agaagcccga acttctcgtg
    10501 tggttttcgc aaggcccgtg ccatagatgg aaatgatcgt ccccggtgcg acgaagttat
    10561 tgggcgcagg gatgctgctc gcagcgttta cgacgccgcc cacattgatc agcggggcct
    10621 cgggactggg gaccccatcc gcagcagtca gaactgccgc tgcggccgcc gtagctagca
    10681 ggtaccacca acccggccgc atccggtgcg ctgctcgatt tgtcggcttg caacgttgcc
    10741 gatgcaaacg tcgtcgccgg cagctcaaga cattgtacac ctcttccacc caagagaaag
    10801 agaagaagac tgaattgcct tccggcaagc tgtcaattca atcgccggcg ctcatcgctg
    10861 cactgttatg aacgcgcccg gcggcgtgga aaccccattg atgctcgcgg aaatgggaag
    10921 gtccccatcg ggcatgtttg cggggatatg aatattaatc tggtaaagtc cggcgaagcc
    10981 tggagctacg cccgcataga gcacgtcgga atcagcgagc accacgttgt tgagcgtcac
    11041 ggtgaccttg gataccgttg caccaatcgc atcgggcagc acgccggccg caaagctcgg
    11101 attggtggcg ccgaaccctg tgccgtaaag cgctacgaaa tcgccgggtt tggccggagc
    11161 gaaaaccgct ccaggcaaca gcccaggcgc tccgatgaga gatcctgtga ttgcgttggt
    11221 cgccgccacc gggctggggc cgcccgagtt gtgcacaaag aagaagaact ccggcgcaac
    11281 cggctgcatc accactgtct gcgtgttgct gatcacttgt ctcgatgctc cgcagttttg
    11341 aatcacctgc actcccaccg cgccggtgct cgaaagcgac ggcgcctgcg cgttgatctg
    11401 cgtgggcgag acaaaaaaca tcggcgccgg ccgggagtcg atttgcacgc acacccccgc
    11461 caggttcgac ggcaaatggc cgtcgaccag atcgcctgcc gttacgccgc gaagcacgcc
    11521 gggcgcagcg aaattctggc cgaagatgct gatcaatccg ttcggtgaga tctgcgcgac
    11581 cggcggattg ctcaatccgg cgcccacgat tccaccggag ctgatttcgg gcggtggccc
    11641 cgcgccggat ccggccgcgg aaacggtgac gctcgtcgtg taaatgttgt cgccgggatc
    11701 ggcttgacta ttcccgttcg ccgcattgcc cgctgcggcg aaaataatgt cgccaacgtc
    11761 gctgctggga gcggtccaat ttacttgaaa agttacgcct cctcgcgtgc cgagccgggt
    11821 accgggcaaa gtgtgctcgg caaacgatat ggttccggcg ccgcaaggca cgggaccaaa
    11881 gctcgggttc ttcgggtcgc cgcagactac ctgtacgttt ccatcaagcg gttggaagct
    11941 gccggcgctt gtcatcaggc tgttcgctgg ccgcgcggtc aactgaaagc cccaccgctg
    12001 ctcggtggga tccgaaaccg ttaccgtgat ggtctgggtc tcgcctggct tgtacggatt
    12061 cacagaaata ctgaccattc ccggaccctt gtttagccgc gacggcgagc tgtggcaccc
    12121 tgattccgcg caactggatt cgccaggaaa cggccctcct gttttaaagg gatccggtcc
    12181 ttcgctgtat gaccaaagcg cgggaagaag cgccccgcag agccctaaga ccaaagcgca
    12241 acggccggag cgctttgaaa tttctgtatt catggtccct tattgaaacc cgcaccggct
    12301 gcaaaaggtt tcactgccca catccgaaga ggcgggattt acaacaagaa gagtggacaa
    12361 gaaatttcga gtcgcggctc gcgctgcgcc atccaagcag catgcctgcg caggtgcagt
    12421 agagtgtaaa cgtggctccg atccaaaaaa tgtgcaaaag tgaaccggag ctcgggccat
    12481 agcaggctct tcatggaagg gatgccgaaa caactatgcc tttgctgttg acggttctcc
    12541 aaggccccgg tgggaagagc gagaaggatc caatttgcgg catgcaggtg gacccgctgc
    12601 acgccgccgg aaggcacgaa cacaaaggga gggtctatta cttttgcagc cgtagctgcc
    12661 tggaaaaatt ccgcgctgaa cctgagaaat acctgaagcc ggcccccgcc gcgccgccga
    12721 tcgcccgtcc gccggctgcc aaagcgccgg ccgaatacgc ctgccccatg catccggagg
    12781 ttgtgcgagc cgggcccgag gcatgcccgg cgtgcggcat ggcgctggag ccgcgaacac
    12841 cactgcgcgt ggaatatgtt tgcccgatgc acccgcagat cgtgcggagt gcccctggca
    12901 cttgtccgat ctgcggcatg gcgctggagc cgcgcaccac gctgttggcc gaagagtcca
    12961 acccggagct ggtttctatg actcgccggt tctgggtgag cgcggtgctc acagcgccgc
    13021 tgctgctctc catgattttc ggccatctgc ctgcgctcgc gcagcttgct ctggccactc
    13081 cggtggtggt ctggggcgca tggcccttct tcgaacgtgc ttgggcctct ctggtaaacc
    13141 gcagcctcaa catgttcact ctgatcggca tgggcacggg agccgcttac ctctatagcc
    13201 tgatcgctgc gctctggccg ggcatttttc cggaagcttt tcgcagccac actggccgtc
    13261 ccgaagttta tttcgaagcg gccgctgcca tcacgacatt ggtcttgctt ggacaagtga
    13321 tggaactgcg tgctcgcggc cgcacctcgg cggccatccg gtctctgctg aatctgacgc
    13381 cgaagaccgc ccggctggtt cagcccaatg gagatgaaca ggatgttccg ctggatcata
    13441 tcaggcccgg agaccggctt cgcgtccggc cgggcgagcg cgttcctgtt gatggccgca
    13501 ttctcgaagg cagcagctcg atcgacgaat cgatgctcac cggcgagccg atcccggtcg
    13561 agaaagggcc tggcgatcgc gtgaccgggg gcgccgtgaa cggctccggc agcttcatca
    13621 tgcaggcaga gcgcgtgggc gctgaaacga ttctggctca aatcgtgcgc caggtcagcg
    13681 aagcccagcg cagccgcgcg ccgatacagc ggctcgccga taaggtttcc caggtattcg
    13741 ttccggccgt ggtcctggct gcggcaatca cttttgtcgt gtggtctcaa gccggccccc
    13801 agccccgtct ggctcacgct ctcgtcaatg ccgtcgcggt gttgatcatc gcctgcccct
    13861 gcgcgctggg tctggcgact ccgatgtcca tcatggtcgg gataggtcgc ggagccactt
    13921 cgggcgtgct gatcaggagc gccgaggcgc tcgagatttt tgaaaaagta gataccctcg
    13981 tcgtcgataa aaccggcact ctgacccaag gcaagccgca cctcaaggcg gcaatcgctt
    14041 ttcaggaggg gaaatttctc gaggaagatt cacaaagcgc agccgaaatc ttgcgcctgg
    14101 cagcgagcct ggaaaaggca agcgagcacc ctttggccgg cgccatcgtt tcggaagcgc
    14161 agaagcgcgg cctgccgctc gcttctccct ccgattttca gatgattccc ggcaagggag
    14221 ttaccggaac agtggagggc cggcgcgtcg ccctgggcag tttcccgttg atggaggact
    14281 tgggcgctgg cgccgagccc ctcttcgagc gtgcccgcgg attccaatca gacggcgcga
    14341 ccgtgctgct ggccgcgatt gacggccggc ctgccggcct gctggcggtg gaggatccga
    14401 tcaaggagtc gacccgcgaa gcgctcgcag cgctccatcg cgagaaaatt cgcgtcgtca
    14461 tggtgacagg cgatgatccc gcaaccgcca aagccgtggc caggaaaatc ggcatcgacg
    14521 aggtggaagc cggcgtgctt cccgaccgga aaggcgaaat cgtcaagcaa cttcaagccg
    14581 aaggacggat tgtggctatg gcgggcgacg gcatcaacga cgctcccgct ctggcgcagg
    14641 cggacgtagg tatcgcgatg ggcacgggcg ccgacgtggc catagagtcc gctggaatta
    14701 ctttggtcaa aggggatttg cgcggcatcg cccgcgcacg ccggctcagc gccgcaacga
    14761 tgcgcaatat ccgccagaat ttgtttttcg ctttcgttta caatttgctc ggcgtgccga
    14821 ttgcggccgg cgtgctttat ccggcattcg gattactgct gaatccgatc attgccagtg
    14881 cagccatgag cttcagctcg gtatcggttg tcgggaacgc tctgcggctc cggcacatct
    14941 cgttgtgaac aaacccccgg gctgttgctc gatcggctcg ctggcctctg gcgcgcgccc
    15001 gcaaaagcac tcagatcccg ccgccgggat tgcccacatc ctcgccaatc cctgcgccgc
    15061 cggctggctc gatcctcgct tgcctgcgct acacggcggc tatggtcttc agcgccttct
    15121 tggtgatggt ggttagcgga atctcgggca gttcttccac cgcgcgccat cgaaagcctt
    15181 gcggagtcgg cccccgcagc gccgcccgaa acaccgttat cgtgtagttg tgatatgtaa
    15241 ttgagtgccg gaactcccgc agccgctctt tcaacgtcag gcccaaggac gcgaggcaat
    15301 ccggatcggg ctgctcgttc tccgcttggg gcagctccca aaatcccggc atctgggaac
    15361 tatgcggcgg ccgctgccga agcagcagct tccgccccct ctcgacgaca gccacggcaa
    15421 tccgcagccg caccggcctc cgggattcca gccgcacggg caggccgttc tgcgttccga
    15481 ggagcaaagc ctggcaatgc gctgctaccg ggcacagcga gcagcgcggg ttctgagcca
    15541 cgcagatcgt tgccccgagt tccatcagcg cctggttaaa atttccggcc gtattggcgg
    15601 tttccggggt atggctcccg cccttgccgc cggatcgggt cgcttcgagc cacgcctgag
    15661 cgcggcgccg cagcgcctcc cggacgcgcg cggagcggat gtcgccggag tggtttgtca
    15721 tcctggcgag cacccgcgcg acgttgccgt cgagtaccgc caccggctcc ccgaacgcga
    15781 tgctcgccac tgcggctgcg gtgtaaggtc cgacccccgg cagttccttc cagtccgcat
    15841 accgggacga aaaggcgccg cccattctcg aaacaatgta tcgcgcagcg cggcgcaggt
    15901 tgcgcgcccg ggagtagtag ccgagtccgc tccagtatcg gagcaactcg gtttcgctcg
    15961 attgcgccag cgaattcaga tcggggaagc gccgcaaaaa ccgctcatag taaggcacgg
    16021 cggctgccgc ccgggtctgt tgcagcataa cctccgagat ccagatccgg tacgggtcag
    16081 aggtatttct ccagggaagg gggcggcggg tgcggtgaaa ccaagccagc agttttcggc
    16141 cggaatcatc cggcgcagca acagcttccg cggatgatga ctttccggaa ccttttgcgt
    16201 ctaacttcgt aaacatgcac agaagtgttg ggcgcgcctt cctgaggcgc cggccaacat
    16261 gcagcaaccc gcctgaactc aaaacccggc ggattttcct ccgaatccgc cgggcccttt
    16321 ttttcgcttg ttgtgtgtga gtgattcggg caggcgctgg aagaacgcgc tggccgacaa
    16381 ttcacttctt tgcgggagtc gcgtttgcgg ccggcgccgt ctttttcgcg gttcccgtct
    16441 tgcccggggg cgcggcgccc ttggccgccg cggccaaggg ataacgctgg atccgaatgc
    16501 tgcgaattac cacaggcgtg cgcggcttgt cattggaatc gcgcggcacg ttggcgatct
    16561 tggtaaccag ctcctgccct tccagcacct ggccgaaaat ggtgtgatgg ttgttcaggt
    16621 gcggcgtcgg cacttctgtg ataaagaact ggcttccgtt cgtattcggc ccggagttgg
    16681 ccatcgccag ccgccccggc tggtcgaact tcaaagaggc cacgaactcg tctttgaatt
    16741 tgtacccagg gtcgcccatc ccggttccca gcgggtcgcc gccctggatc atgaagcccg
    16801 ggatgacgcg atggaaaatt gtaccgttgt aaagggggcg cttcaccttc tgcccggttt
    16861 tgggatccgt ccactctttg gtgccgcgcg ccaggccgac aaaattcgcg actgccagcg
    16921 gcgcttcttt ctcgaatagt ttgcagacga ttggtcccat cgatgtattg atggtggcat
    16981 aaaggcccgg ctcgcgctcc gccggcgcgg ctggcgccgt tttgggctgc tcctgcgccc
    17041 aggcgagcgc cgcagacgcc agcaaaataa gcaatcttgt tttcactttc ccttctccat
    17101 ttcctcgtgc tgggcgcagc agtgccgcgc gcctacgaaa acgcattata accgcatcga
    17161 gcgtccaacg cggcatccag ccggggcgat ggtccctgcc cgatcaaagc ggcagggaaa
    17221 acctgctttc cggccgccat cctcatgagc ttataactcc tggtgcagag ttcccagcag
    17281 ccggtcttcc caggaaaaat ccggctccgg ccgtttctga gccaaatacc actgaaacgt
    17341 ctcccggagt cctgcgacaa aggctgtggg ctcgaaaccg agctcgcgcc gggcctgtgc
    17401 aatagatacc gtaatcgaag gcagatcgag gtattcgccg aaatacagag gcggctggaa
    17461 cacgtttccg ccggcggccg caatccgccg ccggtggacg aagaccagcc tggcgggaag
    17521 ccccgccgcc gcggccagtg cctgtacggc ctcaacttgg gtcacgggtg gggcatgagc
    17581 aatgttgtaa gcttggccgg gcgaggcagg ggcttcggca gcttgcaggg cggcccagac
    17641 gagatctttt acatagacga attgcatgag cctggcccca tcttccggaa taagcaccgg
    17701 ccgtcctcgc acaatccgat cccagaaaaa agactcgcgg tagtacggat tttccggccc
    17761 gtaaatgaac gggggccgca gggtcacggc gggaaagccg tgttctcgat gcatggagaa
    17821 caacatctgc tcggattccg ctttgttgag cgcgtattcg ctggactggc cggcagagac
    17881 gagccgggcg ccctcttccc aatccctacc ctcggggtag acggccacac tggacataaa
    17941 aacataccgc cggacatgtc ccgcgcacgc ctcggctgcg gcctgcacct gcgcggcggt
    18001 tgtgccgcgc tcagcgtcgt aaacgttatc gaagacaagg tcaaaccccc ggtctgccag
    18061 cgcccggcgg atggcgccgg tatcgttgcg gtcacaaagg atttcgctcg ccccggcggg
    18121 aattaccgat ctgccgcggt gcaaaatggc gacgcggtcg cctcgcgcca gcagtcggcg
    18181 cacaagctcc ctgccgatga actgcgttcc gccaatcacc agtacgcttc ttgacgccgt
    18241 catgacagga acgtccgcca gcttacatct ttcttgaaaa taggaccctt ggcaaccgtg
    18301 gctggtaggt ccggagggcg catgatatac ttttgacgcc acccggaaag acatgtcgca
    18361 tacggccgag aagcagattt ctgccccgag cctctctacg ctggtagttg acgacgagca
    18421 actggcccgc gaggagttgt gctacctgct gcgcgaatgc ccgggcatcg acttggtcgg
    18481 cgccggcacg aatgggctcg aagcgctcca actgattcac gcccatgagc ccgatctggt
    18541 gctgttggat gtccaaatgc cgggcctgga cggaatgggc gtggtgcggc gcttgcaaaa
    18601 tcagggtgga gtgctgccgc acatcatctt cactacagcc tatgatcaat acgctgttga
    18661 ggccttcgaa gtcaacgccg tcgattactt gttgaaaccg atcgacaagt cccggctcca
    18721 ggtgtccctg gaccgagtcc gccgcctggt cgagtctcgg ctgggggcgg gcgccagaat
    18781 cgaagccgag gctgttcccc aggccgcatc ccgtcccggc gcgcgcacca agctgctggt
    18841 aaagcaggcc gggcggcttt ttctcgtcga ttccgaagat gtggtttacg ccacgattga
    18901 tgacggcctc atcaccatcg tggcaacgat gatcgaaggg caatcgaact accgcacgat
    18961 cgaggagttg caatcgagcc tcgatccggc gagtttctgg cgcgcccacc gctcctatct
    19021 tgtgaatatc aacaaaatca aagaagtcgt accctggttc aagtcgagct accagctccg
    19081 gatggacgat aaaaaacaca cggaaattcc cgtgagccgg gcccaaaccc gccggcttcg
    19141 ggaattgctg aaactgtgag gccggccatg acaatcctac ctgtcgagaa ggaaaagctc
    19201 cccgaatcca ttcggcccat ctaccagaac atggaacaga agaccggcaa ggtcctaaac
    19261 ttcttcaagt ttatggcgaa caagccggat gcgatgcgcg cgttcctcga tttctacaag
    19321 gccgtctggg cccccggggc gctctccccc aaaatcaagg agctggccta tctggccacc
    19381 tctgttagca attactgcga gtattgaagc cgcgctcata ccgcttcggg aaagcgggaa
    19441 ggcgtgaccg aagagcagat ccaggcgtta aaggaccctg ggggcgtgca gcgggaggtc
    19501 tttaccgcgg aagaacgcgc tgcggtacgc tacgccaggc ttctgaccaa gcatccatcc
    19561 ggggtgcgcc aagaggatct ggatgagctc ggcaagcact ttaacgctga gcaaattgtg
    19621 gaactcaccc ttgtcattgg cgccgcgaac ctgaccaacc ggtttaacga cggcctgcgc
    19681 gtgcctgtgg acgtgtagcg gaggttcgtc taacaagcgt tgccgaaacc tattcgtctc
    19741 gctcgaccac gtagacctgt gtaaagccgg tgcggtcgct tgcgaaagca accagctttt
    19801 catcgggcga aaaggacggg tgggggtggg tccattgcgg cccgtagacg gtgtcgacgg
    19861 acatttccat ccagctcaag gcgttctctt tttcggatcg cgccgcggcc caatcctctt
    19921 ccagggcgta gcgcgatttt cgccattggc tgccctgatt ggaggcttcc ggatagcaaa
    19981 gggtctttcg ttctccgctc gccacacgga tcagttgcac tccggcatcc ggatgattcg
    20041 tatcgcagag gacaactgtg ccggctcggt ccgccgcgat gtgccaggca ttgcactcgg
    20101 cgatggtttc gatggcccgg gttcgccagt tcatgcgctt cagggcccgg ggccacaccg
    20161 caaacaccag gtcccccgtg agcccaagaa acgtttcgtg aacgacgaac tcgtcgttgg
    20221 tgttttcgta caggcattcc aggccggagc catcgcggcg cacccggtgc atgcgcggag
    20281 ccggatcgcc ggcgaactcg atccactcag gctcaactgg agagaattgc ggatgaatga
    20341 cggtccgggg aaatggaatc acccgccagg cgtttccatc ggcccggcct acggcgatac
    20401 cggcgctgcc gccccgcttg caggcggccg tgatccaaca accatccggg ctcaaggagc
    20461 attctcccaa ttgggctccc gatagctcga aaataagctg ctcttccagc gttgcgcggt
    20521 ccactgccca aacgctgccg ccgcggacga agaacacgcg gctgccatcc ggcgcaatgg
    20581 ccggcgagaa aggatggatc ggggggccgt ccgtaagctg ccggatgccg ccggccggga
    20641 aagccgcttc gaatagctgc ggcgcgccgc tgcggtaaga cgtgaacaag agcgctttct
    20701 ggtccggagt aaacgatgaa tgcaaaaaat aggatggatg attgatcgag gggtgattcg
    20761 tgacctgatg aacgacagcc ccggtccttc gatcccggct gattgcgtgc tccggcggaa
    20821 acaacgctcc cttacgccac atggaaaaaa ccgttcctcc ggggaaatgc tttggtgcaa
    20881 aataacagca tgcctcgtta tcgtatccac tacctgaaag agccgcaacg gctccatttc
    20941 cggcaagcgg ggccgtcgcc cgccccggcg cggctgaaga agaaggatta cgaagccggc
    21001 ggcgagattg aggccaccag cccttacgca gcgtggaaga aaatgcgcgc ggatggagcc
    21061 gaacacccta ttgatgtggg ggacgcgctc gagacggaag ccggaacctt gctgctatgc
    21121 aaatatgtgg gatttgaaga ggcccaatgg atggcgccgc cttccgaaag tgcgggtaac
    21181 gacgacacag cctcgctagc tcacgcgccg atcgaaacgc cgttgtaaga cggctttcgg
    21241 tcttgtctca tcccgggcga gtcaggcccg ggcgcccgtt cccccgatcc caaagcagcg
    21301 ccgcatcgtg ctcagaaagg cttcggctac cgggcatatg tatttgtggc ggtggtaaat
    21361 cagtcctaga tcccggtaaa tcttctcttc gagcggaacg accttgagaa ggccggcggc
    21421 ctgctcggcc tgcgcaaagg cgctgctcat aagcgtgacg ccgatgccgc tggcgacgaa
    21481 tttcttgatg gtttccacgc tcgccagttc catcgaaatt ttcagccggt cctggaagcg
    21541 gcgcagatgg cgatcgagtt ggaggcgggt ctggcccgtc tttggataca acagcggata
    21601 ctcgacgact tcttccagct ttaccgactc ccgccgtgcg agcgggtggc cgggcggagt
    21661 cagcaccgcc atttcatcgc gatgaatcgg caaaacgttc aggtgggcgt gcttgaaagg
    21721 cagcgtgacc acgccgaaat ccacggtgtt ctcgaggacc ttctcgataa ttttccgaga
    21781 gaagttgcgg gtgatttcca ggcggacatc cgggtaatcg cgcttgaatt gaaggatggc
    21841 cgccggcagc acgtacagca ccgtggcttc gttcgccgcg atcgctaccg tgccgcgcgg
    21901 acgccgccgc atcagatcga gctccgccaa agcggcgtcc cgcaactcgc gcaggcgttc
    21961 gccgtagcgc agcagggttt tgcccgccac ggtcagctcg acttgcctcg cgtgccgcac
    22021 gaacagcgcc tgtccgcact gtttttcgag cagccggacc tgcgtgctca cggccggctg
    22081 cgtccggtag aggcgctgtg ccgcccgcga gaagcttccc gcgtgggcca cctcgacaaa
    22141 agtggcaatc tggtcaaaat ccacggcgtt cttgcacggc cacgacagcg tgcatatact
    22201 gaaaaatcat tgtttcacaa tcgctccgtc gccgctatgg attttgacca gcttcgcagc
    22261 ttcgttgaag tcgcccggtt aaagaatttc tcccgggcgg cgggaaagct gggcttgacg
    22321 caacccgcga tcagtacaca gatccgtcag ttggaggatg agttcggggt acgcctattc
    22381 gaccgcatcg gcaaaaaagt cttcctcacc cagcccggtt tgctcctgct ggaccacgcc
    22441 ggc
//
LOCUS       DASREK010000231         4596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1590470, whole genome shotgun sequence.
ACCESSION   DASREK010000231 DASREK010000000
VERSION     DASREK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590470"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFA54_12090"
     CDS             <1..309
                     /locus_tag="VFA54_12090"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZS51597.1"
                     /translation="DNVGGISGAMRVGLLADAFNLECTPHNWGNVMDLAVHFHLELAL
                     PNAYWFEMPHPAHLADRPYQRHRFRIDKDGYVNAPTEPGLGYPIDHDALDRMLKRIDR
                     "
     gene            320..1330
                     /locus_tag="VFA54_12095"
     CDS             320..1330
                     /locus_tag="VFA54_12095"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZS51598.1"
                     /translation="MAIARSDRREFLQTLASGALSFARPGRAAAAAGSAGKQLRGVFP
                     IGQTPFTEDDKLDLECLAREVQFCNRGGVHGFVWPQIASGWSNLSHQERLDGAEAILA
                     AGKGGKTALVIGCQVQGADLARSIQYAKHAAKHGADAVISLPPEKGSEKDYFEFYKML
                     GAATELPLIVQTVGNVSIDLLVEMYKAIPTMKVVKDEAGNPLERVTEIRQRTGDKLAV
                     FSGQGVRTMIEEMRLGFAGHCPTTGLADIYAQAFDLWHAGKRLEAFDMFGRILAFGSI
                     PGAQNYILTARGVFKETTRTRPTPGMGGGPGGQRQQPLTEERKRVIREALDTYLRSYL
                     RA"
     gene            complement(1797..3095)
                     /locus_tag="VFA54_12100"
     CDS             complement(1797..3095)
                     /locus_tag="VFA54_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51599.1"
                     /translation="MKFRIPKFRYVIAALLLLSTTINYADRLALSVVSPSIRREFGMT
                     EQDYGQILFFFLAAYAIMYALSGPIIDRLGTRRGFAATIFVWSSAAMSHALARGKWSL
                     AACRFLLGVGEPGNWPAAAKTVAEWFPADRRALGMGIFNSGTSLGSALAPPVVSALTL
                     AYGWRSAFLWTGAVGFLWLALWLVLYQPPHLSRWITAGEWNFLKDKVRPPEEAVPARL
                     PKREWIRVMARPECYSLIIARFFTDPVLYFIIFWLPEYLSKERHYDLSMIGKYAWVPF
                     IFGDLGYLLGGWLSSWLISRGWSLPRARKLGLICGAAVMPAGIFAPLASGGASAIALT
                     CFMTLGHAVFVANLQTLPTDLFPGPQVGTASGFTGMGGAVGGMIANLSTGYVVQKFSY
                     KPIFAIAGLMHPLATLIIFRLLPDWRFSRREARAPEALKA"
     gene            complement(3232..4461)
                     /gene="hflX"
                     /locus_tag="VFA54_12105"
     CDS             complement(3232..4461)
                     /gene="hflX"
                     /locus_tag="VFA54_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZS51600.1"
                     /translation="MREEPAGGPEESLNELAELARSAGADVLDRLVQSRETPEAATLI
                     GRGKIEELRARARALGADVVLFDRDLTPTQQRNLERELGVKVIDRTQLILDIFARRAR
                     TREGQLQVELAQLNYLLPRLAGRGVEMSRLGGGIGTRGPGETQLETDRRRIFRRIRHI
                     KEQLEAVRASRGRQRSKRVAVPLDTIALVGYTNAGKSTLFNALTSAGVLESSRLFATL
                     DPTVRSLELPSHRRVLVSDTVGFIRRLPTTLVQAFRATLEEVCEAALILHVVDLSSPV
                     RREQEAQVEKVLAEIGAQDKPRVLVLNKIDLVPESERAAVTEAEKQAWPSSPVVAVSA
                     LTGEGLPALLEVLDRSPALEPITRAHFHFDQREGDKISYLHDHGLVLETRYTDSGVDI
                     LATVTDSVRRRLQENLS"
BASE COUNT          952 a         1380 c         1457 g          807 t
ORIGIN      
        1 gacaatgtcg gcggcatcag cggcgcgatg cgcgtaggcc tgctcgccga cgccttcaat
       61 ctggaatgca cgccgcacaa ttggggcaac gtcatggatt tggccgtgca tttccatctg
      121 gaactcgcgc tgccgaacgc ctactggttt gagatgccgc atccggcgca cctggccgac
      181 cgaccgtacc aaaggcatag attccgcatc gataaggacg gttacgtcaa cgcgcctaca
      241 gaaccgggac tcgggtatcc gatagatcat gacgctctgg atcgcatgct gaagcgcatc
      301 gaccgataag ggggaaggca tggcaatcgc acgatcagac aggagagaat ttctacaaac
      361 actggcgtcc ggcgcgctga gtttcgcccg gccggggcgc gccgcggctg cggccggcag
      421 cgccggcaag caattgcgcg gtgtttttcc gatcggccag actcccttta ccgaagacga
      481 caagctcgat ctcgagtgtc tcgcgagaga ggtgcagttt tgcaaccgcg gcggcgttca
      541 cggtttcgtg tggccgcaga tcgcgagcgg ctggtcgaat ctttcccacc aagagcgcct
      601 ggacggtgcg gaggcgattc tcgcggccgg caaagggggc aagaccgcgc tggtaatcgg
      661 atgccaggtg cagggcgccg acctggccag gtcgatccag tacgcgaaac acgcggcaaa
      721 gcacggggct gacgctgtga tttctttgcc tccggagaag ggcagcgaga aggactactt
      781 tgagttctac aaaatgctgg gagcagcgac ggaactgccc ctcatcgtac agaccgtggg
      841 aaacgtcagc atcgatctgc tggtggagat gtacaaagcg attccgacca tgaaggtggt
      901 caaggacgag gcaggcaacc cgcttgagcg tgtaaccgaa atccggcaga ggacgggcga
      961 caagctggcg gtgttttccg gtcaaggcgt gcgaaccatg atcgaggaga tgcgcctggg
     1021 gttcgcgggc cactgtccca ccacggggct ggccgacatc tatgcgcagg cgttcgatct
     1081 atggcacgcc ggcaaaaggc tggaggcgtt cgacatgttc ggccgcattc tggcttttgg
     1141 ctccatcccc ggcgcgcaga actatatcct tacggctcgt ggcgtgttca aagaaacgac
     1201 cagaacgcgt cccacgccgg gcatgggcgg cggccccggg ggccagcgtc aacagccgct
     1261 caccgaggag aggaaaaggg tgatccggga ggcgctcgac acctatttga ggtcctacct
     1321 gcgggcatag cggctggcct ggatttcgcg cggctccggg ctccggcgga aggccgccaa
     1381 cctgcgctga aaccgttggt gaaagccgat gtttttccgg gagaggccgt ccttgacgtc
     1441 ctgtttccgt ccggcgaaac atccaaaaca gcggatccct cgttgccccc gcgcaaccaa
     1501 cgggcggcgg gctttgatct ttgatctgga ttgcaccagg aaacatccag gcccctggcg
     1561 cgcgagatca cccagtgaac gaagcgcaga atccgccctg ccgttttcgg cggccgcccg
     1621 ccgctggctt cgagggcaac gaagttcatg gctgcaaggt tgcggagggt cgtttttctt
     1681 agaaacaaag ccgaactggc ttgcggcaag caattgccct tatcgctagc aaacatctta
     1741 taggcaacat gttttaattc agaagcggct tctggcgaga ggcgccggga gaaaagctag
     1801 gctttcaatg cttcgggggc gcgggcttcc ctccggctaa accgccaatc cggcagcagc
     1861 cggaaaatga tcaacgtggc gaggggatgc attaggccgg cgatggcgaa tatcggtttg
     1921 taggaaaatt tttggacaac atagcccgta gagagattgg cgatcatgcc gcccaccgcg
     1981 ccgcccatgc cggtgaagcc gctcgccgta ccaacttgcg gtcccgggaa tagatcggtg
     2041 gggagggtct gcaaattggc cacaaacacg gcatggccca gcgtcatgaa gcaggtcaag
     2101 gcgatagccg aggccccacc ggatgccagc ggcgcgaaaa tgcctgcggg catcaccgcg
     2161 gcgccgcata tcagaccgag tttccgggcc cgcggcaagg accagccccg gctgatcagc
     2221 cagctcgaga gccagcctcc caacaaataa ccgaggtcgc cgaaaatgaa cggcacccag
     2281 gcgtacttgc cgatcatgct caaatcgtag tgtcgctctt tgctgagata ttccggcagc
     2341 cagaaaataa tgaaatagag aactgggtct gtgaagaaac gcgcaatgat aagcgagtag
     2401 cattcgggac gcgccatcac ccggatccat tccctcttcg gcagccgggc gggcacggct
     2461 tcttccgggg gacgcacttt gtctttcaga aagttccatt ccccagcggt gatccagcga
     2521 gaaaggtggg gcggctggta cagaaccagc cataaggcca gccaaaggaa gccgacggcg
     2581 ccggtccaca aaaaggctga acgccagcca tacgccaggg tgagcgcgga tacaaccggc
     2641 ggcgctagcg ccgaaccgag cgaggttccc gagttgaaaa ttcccatccc gagcgcacgc
     2701 cggtcggcag gaaaccattc cgccaccgtc ttagccgccg ccggccaatt gccgggttcg
     2761 cccacgccca gcaggaagcg gcaggctgcg agcgaccact tgccccgcgc cagagcgtgc
     2821 gacattgccg cagacgacca aacgaaaatt gtggcggcga agccgcgccg cgtgccgagg
     2881 cggtcgataa tggggccgga gagcgcatac atgatcgcgt aagcggccaa gaagaagaac
     2941 agaatctggc cgtaatcctg ttcggtcatg ccgaattccc gccggatgga cggcgagacg
     3001 accgagaggg cgagacggtc ggcgtagtta atggtcgtcg aaagaaggag cagggccgca
     3061 atgacgtatc ggaatttcgg tatgcggaat ttcatgcgcc cgcctggagc gtccggacgg
     3121 gccgttgttt ggcccctcca tcggcaacag catcatactc tcaaattgca gaagccgatt
     3181 actggggaaa ggttttttct ggaagatcgc cggtgaaaaa caagagcgct tctagctcaa
     3241 attttcttgc agccgccggc ggaccgaatc ggtgaccgtg gccaggatgt ccacgccgga
     3301 atcggtgtag cgggtttcga gcacaagtcc gtggtcgtgc agataggaga tcttgtcccc
     3361 ttcccgctgg tcgaaatgaa aatgcgcccg ggtgatgggt tccagggcag gcgagcggtc
     3421 caacacttcc agcaacgcgg gcaagccttc tccggtgagg gccgacacag cgacaacggg
     3481 agacgaaggc caagcctgct tctcggcttc agtgaccgct gcgcgttcgc tctctggaac
     3541 cagatcgatc ttgttcagta ccagcacacg cggcttatcc tgcgcgccga tttccgccaa
     3601 gaccttttcg acctgcgctt cctgctcgcg ccggacaggt gaggagaggt caaccacgtg
     3661 caaaatcagg gcggcctcgc atacttcctc aagcgtcgcg cggaaggctt gcacaagcgt
     3721 tgtaggcagg cggcggataa agcctaccgt gtcagatacg agcacgcggc ggtgcgacgg
     3781 cagctccagg ctgcgaaccg tcggatcgag cgtcgcgaac aagcgcgagc tctcgagcac
     3841 gccggccgag gtgagggcat tgaaaagcgt cgatttaccc gcgttggtat agccgaccag
     3901 agcgatcgta tcgagcggca ccgccacgcg cttcgagcgt tgccggccgc ggctggcccg
     3961 aaccgcttcg agctgctcct tgatatgccg gattcgccgg aaaatcctgc ggcggtccgt
     4021 ctcgagctgg gtttcgccgg ggcctcgtgt tccaataccg ccgccgagac gcgacatctc
     4081 tacgccgcgg cccgccaggc gcgggagcag gtaattcaat tgcgccagct caacttgcaa
     4141 ctggccctcg cgggtgcggg cgcggcgggc gaagatgtcc agaatgagtt gggtgcgatc
     4201 aatcaccttc acgcccaact cgcgctccaa attgcgctgc tgggtcgggg tgaggtcgcg
     4261 gtcgaacaag accacgtcgg cgccgagagc ccgggcgcgc gcccgcagct cctcgatctt
     4321 cccgcggccg atcagcgtgg ccgcttccgg cgtttcgcgc gactgcacca gacggtccag
     4381 cacatcggcg ccagcgctgc gggccaattc ggccaattcg ttcagcgatt cctcgggtcc
     4441 gccggcaggc tcttcgcgca atacttcttt cttccactcg aacccgacca ggattgcccc
     4501 ttctccgccc cggcgattgg tggatgttat ggcttatgcc tccttgtcat cgttattgga
     4561 tgagcgaggg gccggagcgt ttccagaaga tacggc
//
LOCUS       DASREK010000232        15371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_276646, whole genome shotgun sequence.
ACCESSION   DASREK010000232 DASREK010000000
VERSION     DASREK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 15371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15371
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276646"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="VFA54_12110"
     CDS             <1..1510
                     /locus_tag="VFA54_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51601.1"
                     /translation="DGRALTVDVGGEKRWIAAEDKAHYLPAVDVFVIRRYVETHGPFL
                     MKDLARRYGLSLEAAQALAAGCGVEIAAAGDRCCARDVLRRIQRASLAVLRREVEPRP
                     IEAYTRFALSWQRVTAAPASTLDVLEQLEGLPLPSAAWRQILGARLKDLKEIEELVRS
                     GNIVAAGLRPGWFTLSARGRGGLFAADEPGAVSRCASRLLEHLTEEGPATLADLRRDT
                     DLTLAALNAALAELFWSGRISCDGLREIERLGRPPKGRPEAPALRPVLIDPLERPPRA
                     EAIARFRQAFRRLPGWEGRWFPLRLRRVPEDLRGQEIARVALGRYGVLARECYEHEDS
                     PVSWSDLFPHLERAEWRGELRRGHFLDALSGMQFALPEAVDRLRSAPEAGYVLLNSCD
                     PASPYGARLCGAWLQSVPIRRRPANEVLLDNGTCVLYAENHGARCWVRAGDRAALSAY
                     VDLHTGSGLTIEQINGCPAAGSEFAALLAERGFRRQAGGALRQPFLHRFSLG"
     gene            complement(1579..1998)
                     /locus_tag="VFA54_12115"
     CDS             complement(1579..1998)
                     /locus_tag="VFA54_12115"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZS51602.1"
                     /translation="MTSPAADKDRDREQIRALLEHINDAWLKNPPEKIPALLQEHFHP
                     DMTIKGPDFQSLCIGSESCARSYAEFARTARVKECSFSDPDIDLAGDAAVATYSWKMV
                     YEIHGQEYNESGRDLFVMSRTKDRWVVLWRALLAGMQ"
     gene            2201..3511
                     /locus_tag="VFA54_12120"
     CDS             2201..3511
                     /locus_tag="VFA54_12120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51603.1"
                     /translation="MSGASQGQVVSPIAERTRRRITRRLMPFLFLLYIVAYLDRVNVG
                     FAGLEMTRELGFSNEVFGLGSGIFFVGYVILEIPGTILVELWSARKWIARILISWGLL
                     ASLTGLIHTAHQFYWIRFLLGMAEAGFFPGIIVYLTHWYRYEDRAKAVAMFMAAIPVA
                     NIIGSPVSGLLLRIHWLGYSGWRWLLLLEGIPALICGAITLFYLTDWPRDAQWLPQDE
                     REWLTAELEREKETKKKVRPMSVWQALRNRDVVLLTFVYFFVVTASYGVNFWLPKILQ
                     RLSGLSPFQTTLITAIPYLCALPAMLAVGWHSDHTGERRWHTALSIYAVAAGLLLSQF
                     VGGSVPLAMAMFSVAVMGLYAYLPGFWALPTTFLTEAAAAACIGLINSFGNLGGFVGP
                     YAVGFLSNRTGTYAAGIYYLVGSAILSGSLVLVLRGTRKLTAEA"
     gene            3744..4628
                     /locus_tag="VFA54_12125"
     CDS             3744..4628
                     /locus_tag="VFA54_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZS51604.1"
                     /translation="MNTPIITPYGADFRPDLGAMRALIDFALANGVRSICVGTTTGEF
                     IHYSLGLRKQMLACAIAHTRAPVVAGIAHSTFDGALELARAAADAGARALLLMPPYFF
                     RYAQDEVREFFLRFAEVRPANLPLLLYNIPAFTNEISAETARELLSTGLFAGIKDSSG
                     SLDFFQRLKALRSQRAFTLLVGDDRIFTEARMQGADGVISGVSGVVPELVIAIERAVQ
                     AGAASRAQSLDRRLREFIAWLDRFPVPAGIKLALAERGMPSGHETVVLGPERRRLGEQ
                     FRAWFRKWLPAVLEEAAA"
     gene            4625..6067
                     /locus_tag="VFA54_12130"
     CDS             4625..6067
                     /locus_tag="VFA54_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZS51605.1"
                     /translation="MKERRLGDMPAEEFRRLVCETAEWMADFLARAGERPVLPSVVPG
                     AVAAALPAQPPDHGEEMPAILADFQRLIFPALTQWNHPAFLGYFATTASGPGILGEML
                     AASINTNAMMWSTSPGATELEQVVLDWLWQLVGLPDHCFGMIHDTASTSTLHAVAAAR
                     EALEDLAIRTRGQSGAPPLMLYTSTHAHSSVEKAAIALGIGQDNVRKIEVDDEFRMKP
                     EALDAAISVDLLAHCRPFCVVATVGTTSTTSIDPVPKIAEICRRRRLWLHVDAAYGGA
                     AAIVPEMSHVLAGCDLADSLVINPHKWLFTPQDLSAFYCRRPEILRRAFSLVPEYLHS
                     GRDARALNFMEYGVPLGRRFRALKLWFVIRYFGREGLVANLREHLRLAREFGARIQEH
                     PDFELMAPVVFSTVCFRFRPQRISETQLQRLNRSLLEAVNRTGQLFLSNTVLHDKFVL
                     RLAIGNLRTTQQHIEKAWELIRSKARELTL"
     gene            complement(6142..6522)
                     /locus_tag="VFA54_12135"
     CDS             complement(6142..6522)
                     /locus_tag="VFA54_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51606.1"
                     /translation="MQGASATEHILDEFAGKPVRAFVIWEPVLLTDWSSPSTATLSRL
                     SDSRVAQFWDKGRLISRSMGEHDRRSIVWDFISIYPAGALWEDKPPEALYAGGPVVKV
                     AGPARAALAQALERQPFRGNGAAR"
     gene            complement(6532..6732)
                     /locus_tag="VFA54_12140"
     CDS             complement(6532..6732)
                     /locus_tag="VFA54_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51607.1"
                     /translation="MKRKLGIAVIFAALLAAVLAFFYGGSQVPAGQSPLRSLTAASPD
                     DVKNEFNLAKNSVRVLLLVSPT"
     gene            complement(6736..7119)
                     /locus_tag="VFA54_12145"
     CDS             complement(6736..7119)
                     /locus_tag="VFA54_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51608.1"
                     /translation="MQDKATDLAAKPAPGSRHAGSHAAAASLAAIAGIMAASSCCLPV
                     FPFMLAAGLAGGSTFLDAARPYLLAGSLLFLAYGFYQAPRARQCRQRGSVISTIVLWV
                     SAGFVFLSIFFPQLMANAAAGLLAR"
     gene            complement(7553..9289)
                     /locus_tag="VFA54_12150"
     CDS             complement(7553..9289)
                     /locus_tag="VFA54_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51609.1"
                     /translation="MLSKCPTITVGSVVLTAFYEPVRRVFPWPVFLLLVSAVIPLLAA
                     APNVAAVGNAASNLTFNSPIAQGAIFVIYGTGLGPDAISISSTPFKSTTLSGTSVAVT
                     VGTTTVNALMYYTSATQVAALLPSNTPTGSGTFTVTYNGQTSNSISHGIAPSNVGIFT
                     IDSSGQGPAIVTYPDFSLVSAAKGANCGGPNTFCGAANPGDVLILWTTGLGPVSGDDA
                     LGAGLGQNMPNVPLTVWLGGVQAPVTYQGRSGCCIGEDQIVFTVPNNVPTGCAVPLVV
                     QIGATTNTVSNTTLMPVAKGGRDCTPTNPVLASVNVEQAVMAGPVAYGAIELNHELNG
                     AAPPVFQDTARFEFFKILSLKPGRQPFFATWIDDQPPGTCRAYNNNLFGGGGTDPTGT
                     VTLLDAGSSFTVKGPNGSVPITGNPGEFSATLSGTGSFLVPGSYTVTGSGGADVGPFS
                     ATITVPRLPTLASPANNTTVTRSDGLTVTWTGGDPAGNVQILVNSALNNSFNAGGTAT
                     CIAPAGAGKFTIPPYVLLALPAGNLAGIVLAPADTGVPFTAPGLSLGLLSTHNDGAGY
                     GLGAGTGGFTLR"
     gene            complement(9435..10568)
                     /locus_tag="VFA54_12155"
     CDS             complement(9435..10568)
                     /locus_tag="VFA54_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZS51610.1"
                     /translation="MAGRQVYADTVLELTGLIYASVADSSCWKVFLEAFLTSIRGQAC
                     SLALRDAADGRLSMNCWYGWSDEDMTLYFESYSAADPWRIANARGPEGAVRAGADLSR
                     REEMEPSAAFREFYAPRGAIHGMGGTILATEGRESIISAVRGACEGPFGEPEKAVLRA
                     LMPHLKRAVLLHGELASLRLQLSLLAGHLDRCSHAFFLTDREGRVLYANAAAREIADL
                     RDGLSLEDGVIVAASVRQNAEFREAVMEAAGGRNGAWRRVTISRPSGAEPYRLILMPA
                     KDSGACPGVSRPAASILVIDSVFSPELDVPVLCELFPFTPAEARVAAKLALGRSVEEI
                     AAELRISVETARTHVKRTLSKTATERQGELISLILRSVPVRRL"
     gene            complement(10777..12222)
                     /locus_tag="VFA54_12160"
     CDS             complement(10777..12222)
                     /locus_tag="VFA54_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS51611.1"
                     /translation="MPETKGKILIVDDDTAIRESLETFLAFEGYQVDLAVDGTSGLLA
                     LESKAYDLVLLDLMMPDRSGLDVLKELRQRDVETPVMVITAYGSVENAVAAIKLGAND
                     FITKPWNNEKLLIEIDQTISKRRLERENTQLKRALKQRYNFSNIIGKSEAMRKVLDLV
                     GQVAQSRSTVLLHGESGTGKELMAKAIHTNSPRAEKAFVAVNSGSLPSELLESTLFGH
                     LKGSFTSAIQTKKGYFEIADEGTIFLDEISTISLEMQAKLLRVIQEKEFVPVGGVDPR
                     KVDVRIVAATNVDLRKQVEEGRFREDLYYRLNVIQIDLPPLRERREDIPALVEHFFTK
                     YCRENDKFLDPDGSSRLRFAPEAMRMLMDYHWPGNVRELENVVERAVVLATDTTVPVD
                     LLPDTLLTINGVPIRRRIPTFTRPPEASLFDLVDEFERRIIIEQLEKYNWSQTDTAES
                     FRIPLSTLNQKIKRLNIEIKRKRDLASAGKG"
     gene            complement(12215..15163)
                     /locus_tag="VFA54_12165"
     CDS             complement(12215..15163)
                     /locus_tag="VFA54_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS51612.1"
                     /translation="MIHDLQFRFSRTLLTLLTVAAGLAAILNFQQQRRFELPEDGVTW
                     VDGQSGVQALRVTPDGPADRAGLKSGDLLLAINGVPVRQALRVPQFLLGIGTWKKAEY
                     RFMRRGVELNSTLIIEPAKRSPILYYLYGLGLIYLLIGLYVFYRREGAPKAQHFYIYC
                     LASFLLYCFHYTGKLNAFDRVMYWSNVWAFLFAPALFLHFCLTFSDNGIRSKRRKAAV
                     AACYAPAVIVFAAYVGAATGAVRTSLPLVELRELLDRISFALLSGCFLAGVLALEAAY
                     RRASAGEASTVRQQLKWLKRGTFWGVAPFLAVYAVPYMLGAAPQAWMGLGVLPLGLIP
                     LTFAYAIVRYRLMDVDVIFRRGFAYSLATMALLGAFYALLFSLGDVIQRNFRDLGQTG
                     WIVAMLAAAFLFQPLRSRIQERLDRFFYRERYDYRRTLVEFARELNAETNLAAMLGSV
                     TERISQTLGIERVAVFLPRETTAAAGAGGSASVPGWELAKGLNLRDRKGRYIQPGDFL
                     DLSFLEAARPEKSAQAKPYLFFESTRWALDNTAPVRSTIADLDLTYYLPCTARGKTVA
                     FLGLGRTCNREFLTGEDLELLQTMVGYLGIAIENARLYQSLQQKVDQYERLKDFSENI
                     VESIHVGILAVDLEDKVESWNTQLELMYGISRDEAVGRPLSALFPEDLVKEFVRVREE
                     TGIHNIYKYRLRPETLPKPPAEPAKDRTLNIAIAPLVAKNFDRIGRLVIFDDVTDRIE
                     LEQQLQQAEKLSSIGLLAAGVAHEVNTPLAVISTYAQMLAKQVSHDEMKARLLDKIAS
                     QTFRASEIVNSLLNFSRTSATDFSRVSLNRVILDTCALLEHQFRKAGIRVDAQLEEGL
                     PEIEGNTGKLQQVFLNLFLNARDAMPAGGVLTVRTAAGSAGGNSGREVVRAEVIDTGE
                     GIAPEHLNRIYDPFFTTKGGQKKGTGLGLAVTYGIIQEHSGAISVESKPGRGTYFRLE
                     FPLARKEVHA"
BASE COUNT         2890 a         4840 c         4656 g         2985 t
ORIGIN      
        1 cgacggccgc gcgttaactg tggatgttgg gggggaaaaa cgctggattg ccgcagagga
       61 caaagcgcac tacctgcccg ccgtggacgt gttcgtgatc cggcgctacg tggaaaccca
      121 cgggccattc ctgatgaaag atctcgcccg ccgctacggt ctttcgctcg aagcggccca
      181 ggcgctggcc gccggctgcg gcgtggaaat cgccgcggcg ggcgaccgct gctgcgctcg
      241 cgacgtgctg cggcgcatcc agcgcgcttc cctcgctgtg ctgcgccgcg aagtcgagcc
      301 ccggccgatt gaggcttaca cccggtttgc tctctcctgg cagcgcgtga cagccgcgcc
      361 cgcatccacg ctcgacgtac tcgagcaact ggaaggcttg cccctgccgt cggccgcctg
      421 gcggcagatc ctcggtgcgc ggttaaagga cctaaaagaa atcgaggaac tggtccgctc
      481 ggggaacatc gtcgcggcgg gactccgccc cggctggttc acgctttctg ctcgcggccg
      541 cgggggcttg tttgccgccg atgagcccgg cgccgtgagc cgctgtgctt cccggcttct
      601 cgagcatcta accgaggaag gcccggcaac cctggccgac ctgcgccgcg acaccgatct
      661 gacgctcgcc gctctcaacg ctgccctggc ggaactgttc tggagcggcc ggatttcctg
      721 tgacgggctg cgagaaatcg agagactggg gcgcccaccc aaaggacgcc ccgaggcgcc
      781 tgctttgcgg ccggtgttga tcgaccccct ggagcggccg cctcgcgccg aggcgatcgc
      841 gcgcttccgc caagcttttc gcaggttgcc gggatgggaa ggccgctggt ttccgctgcg
      901 cctgcggcgg gtcccggaag atctccgggg ccaggagatc gcccgtgtgg cgctcggccg
      961 ctacggggtc ctcgcccgcg aatgctacga gcacgaggat tccccagtga gctggagtga
     1021 tctgtttccc catctcgagc gggctgagtg gcgcggcgaa ctgcggcgcg gccatttcct
     1081 cgatgcgctg agcggcatgc agttcgcgct cccggaggcc gtggatcgcc tccggagcgc
     1141 gccggaggcc ggctatgtat tgctgaattc ctgcgacccc gcgagccctt atggcgcccg
     1201 gctttgcggc gcctggctgc aatccgttcc catccggcgc cgccccgcca atgaagtgct
     1261 gctcgacaac ggaacgtgtg ttctatacgc cgaaaatcac ggcgcgcggt gctgggttcg
     1321 cgcgggcgat agggctgccc tgagcgcgta tgtggatctt cacaccggca gcgggctgac
     1381 aatcgaacaa atcaacggct gcccggccgc gggcagcgaa tttgcagcgc tgctggcgga
     1441 acgcggcttc aggcgccagg cggggggcgc gctgcgccag ccgtttttgc atcgtttttc
     1501 cctgggatag caagcaagac cgtcgctcaa aacggtccca cagcaaaagc aggaccggtc
     1561 cgcgccgtcc cggcgttttt attgcatgcc ggccaaaaga gcgcgccaga gcaccaccca
     1621 gcggtctttg gttcgggaca ttacgaagag atcgcgcccc gattcgttat attcttggcc
     1681 gtggatctcg taaaccattt tccaggaata cgtagcgacc gcggcatccc cggccaagtc
     1741 gatatcggga tctgaaaagc tgcactcctt cacccgggcc gtccgcgcaa actccgcata
     1801 gctgcgggcg caagactcgc tcccgatgca caacgactgg aaatcgggcc ccttgatcgt
     1861 catatccgga tggaagtgtt cttgcagcag agcggggatc ttttctggag ggttttttag
     1921 ccaggcatcg ttgatgtgct cgaggagagc gcggatctgt tcgcggtctc gatctttgtc
     1981 cgccgcaggt gaagtcattt tttccggtcc tcggtccgcg cccaacccgg ggagcttccg
     2041 ggcgccgcag ccgcgccgcc cggcagagcc gccggcgggg cttccgggcg cggcggagcc
     2101 gattcttgat tctagaacgt tttgccgcca tccgcccgcg cttgcacttg ctccgggctc
     2161 cggttccagg tatgcttgac ccttcactcc aagccttctc atgagcgggg catcccaggg
     2221 acaagtggtc tcgcccatcg ccgagcgaac ccggcggcgc atcacccgcc ggctgatgcc
     2281 atttctgttt ctgctttaca tcgtcgccta cctcgatcgc gtgaatgtcg gctttgccgg
     2341 gctggaaatg acgcgcgaac tcggtttttc gaacgaagtc ttcggcctgg ggtccggcat
     2401 ctttttcgtg ggctacgtga tcctcgaaat tccaggaacg atcctagtcg agctatggag
     2461 cgcccgcaaa tggatcgccc gcatcttgat ctcctggggg ctgctggcct cgctcacagg
     2521 tctgattcac acggcgcacc agttctattg gatccggttt ttgctgggca tggcagaggc
     2581 cggttttttc cccggcatca tcgtgtatct cacccactgg taccgctatg aggatcgcgc
     2641 caaggcggtc gcgatgttta tggcggcgat tccggtcgcc aacattatcg gctctcccgt
     2701 ttcaggtctc ctgctgcgca ttcattggct cggttactcc ggctggcgat ggctgctttt
     2761 gctcgaaggc attccggcgt tgatctgcgg ggccatcacg ctcttctacc tcacggactg
     2821 gccgagggat gcgcagtggc tcccgcagga cgaacgcgaa tggctgaccg cggaactcga
     2881 gcgcgagaag gaaaccaaaa agaaagtccg gccgatgagc gtctggcagg ctcttcgcaa
     2941 ccgggatgta gtcttactga cattcgttta cttttttgtt gtcaccgcga gctatggcgt
     3001 aaatttctgg ctgcccaaaa ttttgcagcg gctctcgggc ttgagtccct tccagaccac
     3061 cctcattacg gcgattccat acctttgcgc tttgccggcg atgctcgcag tcggctggca
     3121 ctcggatcac accggcgagc ggcgctggca caccgctctt tcgatctatg ctgtcgccgc
     3181 cggcctgctg ctgagtcagt ttgtcggagg cagcgttccg ctcgcaatgg ctatgttctc
     3241 agtggcggtt atgggtctgt acgcctactt gcccggcttc tgggctcttc ccacgacctt
     3301 cctcaccgaa gctgcggccg cggcctgcat tggcctgatc aactcgttcg gcaatctcgg
     3361 aggctttgtc ggcccctatg cagtcgggtt cctgagcaac cggaccggga cctatgcggc
     3421 tggaatttat tatttggttg gctcggccat tttgtcgggc tcgctcgtcc tggtgctgcg
     3481 cggcacgcgg aagctcaccg cggaagcttg aaaagacatc gtttctccgg aaaggcgctg
     3541 tttctccagc ccaaggggcc gccggccgcc aatcaaacat gacaatctgt gtttcggcgc
     3601 tttcctggct gcatatttcg cgggcatatg ccggaccgct ggcggaagca actccacgcc
     3661 tggcttcccg cgtgtctgtg ggatacttgc gcaacctgta tgcgctgata ttgatattct
     3721 ggtagctcac tatgctccag ggcgtgaaca ctccgatcat aaccccctac ggcgctgatt
     3781 ttcgccctga cctcggcgcc atgcgggcgc tgatcgattt cgctctcgcc aacggagtcc
     3841 gatcgatctg cgttggcacg acgacgggag agttcatcca ctattctctc ggcttgcgca
     3901 agcagatgct ggcttgcgcc atcgcgcata ctcgcgctcc cgtcgtcgct ggaatcgcgc
     3961 attccacctt cgacggcgcg ctcgagctgg cgcgcgccgc cgccgacgcc ggcgcgcggg
     4021 cactgctgct gatgccgccc tattttttcc gctatgcgca ggacgaagtc cgcgagttct
     4081 tcctgcgttt cgcggaggtc cggccagcca acctcccgct gctgctttat aacattcccg
     4141 catttaccaa cgagatttcg gccgaaaccg cgcgggaatt gctttccacc gggctattcg
     4201 ccgggatcaa ggacagcagc ggctcgctcg atttcttcca gcgcctgaag gcgctgcgct
     4261 cgcagcgcgc atttacgttg ctagtggggg acgatcgaat cttcaccgag gcgcgcatgc
     4321 agggcgccga cggcgtaatt tcgggcgtct ccggagtggt tcccgaattg gtgatcgcga
     4381 tcgagcgcgc cgtccaggcg ggcgccgcga gccgggctca atcgctcgac cgccgcttgc
     4441 gggagttcat cgcctggctg gaccgctttc ccgtgccggc cgggatcaaa ctcgctctgg
     4501 ccgagcgcgg catgccttca ggtcatgaaa ccgtcgtgct ggggccggag cgccggcgcc
     4561 tgggagaaca gttccgggca tggttccgca aatggctgcc ggcagtgctc gaggaggccg
     4621 ctgcatgaaa gagcggcgcc tgggcgatat gccggctgag gagtttcgac gcctcgtttg
     4681 tgaaaccgcc gaatggatgg cggatttctt ggcccgcgcc ggcgagcgcc cggtcctccc
     4741 ttcggtggtt cccggagcag tagcggcagc cctgcccgcg cagccgcccg atcacggcga
     4801 ggaaatgccg gccattctcg ccgattttca aaggctgatc ttcccggcgc tcacccagtg
     4861 gaaccatccc gcgtttctag gctattttgc gaccaccgcc tccggccccg gcattctcgg
     4921 cgagatgctt gccgcttcca tcaataccaa cgccatgatg tggagcacct cgccgggcgc
     4981 taccgagctg gagcaggtcg tgctcgattg gctctggcag ctcgtcggcc tgcccgatca
     5041 ttgtttcggc atgattcacg ataccgcctc cacctccacg ctgcacgctg tggcggcggc
     5101 ccgggaagcc ttggaggatc tggctattcg cactcgcggc cagagcggcg cgccgcctct
     5161 gatgctctac acctccacgc atgcccattc gtcggtggaa aaagcggcga tcgctctcgg
     5221 aatcggccag gataacgtca gaaaaatcga ggtggatgac gaattccgca tgaagccgga
     5281 agctctggac gctgcaattt cggtggacct actggcccac tgtcgtcctt tctgtgtcgt
     5341 agcgacggtg ggaacaacct ccaccacttc gattgacccg gtgccgaaaa tcgccgagat
     5401 ctgccgccgc cgcaggctat ggctccacgt ggacgccgct tacggcgggg cggcggctat
     5461 tgtacccgag atgagccatg tgcttgccgg ctgcgatctt gccgactctt tggtgatcaa
     5521 tccccacaaa tggcttttca ccccgcaaga cctgagcgcc ttctactgcc gccggccgga
     5581 aatcctccgg cgggccttca gccttgtgcc ggaatatttg cacagcggcc gcgatgcgag
     5641 agcgctcaac ttcatggagt atggcgtgcc tttgggacgg cggttccggg ccctgaagct
     5701 gtggttcgta attcgctact tcggccgcga agggctggtg gccaatttgc gcgagcactt
     5761 gcgcttggcg cgcgagtttg gggcgcgcat ccaggagcat cccgacttcg agctcatggc
     5821 gccggttgtc ttctcgacgg tttgcttccg cttccgcccc cagcgcatta gcgaaacgca
     5881 gctccagcgg ctgaaccgaa gtctgctcga ggcggtgaac cgcaccggcc agttgttttt
     5941 atccaacacc gttcttcacg ataaatttgt tctccgactc gccatcggga atctgcggac
     6001 cacacagcag cacattgaaa aggcctggga actgatccgc agcaaagcgc gggaactgac
     6061 gctttgaccg gcggagagcg cacggcccag gtccgcctca cggctatcta tcctggctgc
     6121 aaaggaatgt tcgggccgta cctatcgcgc cgccccattt ccccggaacg gctgtctttc
     6181 caacgcctgg gcgagcgccg cgcgcgccgg cccggctact ttcaccaccg gccctccggc
     6241 atagagagcc tccggcggct tgtcttccca gagcgctccg gctgggtata tggaaatgaa
     6301 atcccatacg atgctccggc gatcgtgctc ccccatcgaa cgcgaaatca ggcggccctt
     6361 gtcccagaat tgcgcaaccc gcgaatcgga aagccggctc aaggtcgccg tggaagggga
     6421 tgaccaatcg gtcagcaaca ccggctccca gatgacaaag gcgcgcaccg gcttcccggc
     6481 gaactcatcc agaatatgtt cggttgcaga ggccccctgc aagcagatcg ctcaagtcgg
     6541 cgatacgagc agtagcacac ggacgctgtt ttttgccaga ttgaattcgt tcttgacatc
     6601 gtccggggat gccgccgtga ggcttcgcag cggggattgc ccggcaggaa cctgactccc
     6661 gccatagaaa aacgcgagca cagcggccag gagcgcggcg aaaatcacgg cgataccgag
     6721 tttccgcttc atccatcacc tggcaagcag gcccgccgcc gcgtttgcca tcagttgagg
     6781 aaaaaagatt gagagaaaca cgaaaccagc cgacacccac aggacaatcg tgctgatcac
     6841 gcttccccgc tggcggcatt gccgggcccg tggcgcctgg tagaaaccgt aggcgaggaa
     6901 caaaagggaa ccggcgagaa ggtacggacg cgcggcatcg agaaaagtgg aaccgcccgc
     6961 aagacccgcc gccagcatga agggaaacac cggcaggcag cagctcgagg cggccataat
     7021 gccggcgatg gccgcaagac ttgccgcggc tgcgtgggat cccgcatgcc ggctccccgg
     7081 ggccggcttg gctgcgaggt ctgttgcttt atcttgcatc tctccgcctt ggccgcagta
     7141 tatgcactct cagttccctg agcaagccga cgaggccgcg aacatggacc tactcgtttt
     7201 caggggggct ggtagattac taaaaatccc ttacccgagc ctccgcgcgg gagccgctct
     7261 ttcattctga ggctgagccc tcacatcttt cgaaattggc ccggcataga acccagggat
     7321 ttcactttat cggtgtgttc agagatgctg gcgatccgtt ttgggccggc aaaatcggaa
     7381 ctcaacaaag acagcacgct gttccgcgac cggcgagccc taggctgcgg ctgggcatga
     7441 cgcacgaagc agccgggcga agccggcctg gctcacgcga gggcgtggaa cggcgcagtc
     7501 tgagcggcag tccgcaggca cgcgccaccg cccgatgcgc gcgcccctgc gcctacctta
     7561 acgtaaagcc gcccgtgccc gcaccaaggc cgtagcccgc gccgtcatta tgggtcgaga
     7621 ggaggccgag actgagaccc ggcgcggtga atggcactcc ggtgtccgcc ggcgccagaa
     7681 cgatcccggc caggttgccg gcaggaagcg ccagcaggac atagggcgga atcgtgaatt
     7741 ttcccgcgcc tgcgggcgct atgcatgtcg ccgtcccgcc ggcgttgaag ctgttattga
     7801 gcgcgctgtt taccaggatc tgaacatttc cggccgggtc gcctcccgtc catgtgacgg
     7861 tcaatccatc ggagcgcgtc acggtggtgt tgttggcggg gctagccagc gtcggcaacc
     7921 gagggaccgt gatcgtggcg ctgaacgggc cgacgtccgc tccacctgac cccgtcacgg
     7981 tatacgagcc tgggaccaga aacgaccctg tcccgctcag cgtggcagag aattccccgg
     8041 ggttcccggt gatgggaacg ctcccgttgg gtcccttcac ggtgaaactc gaaccggcgt
     8101 cgagaagagt gacggtacct gtgggatccg taccgccgcc cccgaacagg ttattgttgt
     8161 acgcgcggca ggtgcccggc ggctggtcgt cgatccaagt ggcaaaaaaa ggctgcctcc
     8221 ccggctttaa agagagaatt ttaaagaact cgaatcgcgc cgtatcctgg aacacaggag
     8281 gggcggcgcc gtttagctca tggttcagtt caatggcgcc gtaggcgacc ggtccggcca
     8341 tgacggcctg ctcaacgttg accgatgcca gcacgggatt cgtcggggtg caatcgcggc
     8401 cccctttggc caccggcatc agggtggtgt tgctgaccgt gttggtcgtg gcgccgatct
     8461 gcacaactag cggcacagcg cagccggtgg gaacattgtt tggcactgta aagacaatct
     8521 gatcttcccc gatacagcag ccggatcggc cctggtaggt caccggggcc tgcactccgc
     8581 ccagccacac ggtgaggggg acgttgggca tgttctggcc caggcctgcg cccaaggcgt
     8641 catctccgct gaccgggccc aatcctgtgg tccacaatat caggacatct cccgggttag
     8701 cggcgccgca aaaggtgttg gggccgccgc agttggcgcc cttggccgcc gaaaccaggc
     8761 tgaagtcggg gtaggtgaca atcgccggcc cctggccgct cgaatcgatg gtaaagatgc
     8821 ccacattgct gggcgctata ccgtggctta tcgagttgct ggtctggccg ttgtaggtga
     8881 ccgtaaacgt tcccgagcct gtgggcgtgt tcgaaggcaa tagcgcggcc acctgagtcg
     8941 ccgatgtgta atacatgagc gcattcaccg tagttgtacc gacggtgacc gccacggacg
     9001 tcccgctcaa ggtagtgctt ttgaaaggcg tcgaactgat ggagatagcg tctggaccca
     9061 aaccggtccc atagatgaca aagatcgcgc cttgcgcgat gggcgaattg aaggtgagat
     9121 tgctggcggc gttccccacg gcggcgacgt tgggggctgc ggcaagcagc ggaataaccg
     9181 cggagaccag gagcaggaac accggccacg ggaaaacgcg ccgaacaggc tcataaaagg
     9241 ccgtcaacac taccgagccg acagttatcg tggggcattt cgagagcatc gcccttcctc
     9301 ccgacacaaa ttgaatttct cccagcagcg aaggccactt cacatggcga gtgaagagtc
     9361 aactgctttc acgaaagtat gacgagggga ccgaaacttg tcgtcaccca aattcggtat
     9421 gcaatggact tttcttatag gcgccgtact ggtactgatc gcagaatgag ggagatcagc
     9481 tccccctggc gctcggttgc cgtcttggag agggttcgct tgacatgggt tcgggcggtt
     9541 tccaccgaga tcctcaattc ggcggcgatc tcctccacgc tccggcccag ggccagtttg
     9601 gcggccactc gcgcttcagc aggcgtgaag gggaacagct cacacagcac cggcacgtcg
     9661 agctcgggag agaacacgga gtcaatcacg aggatgctcg cggccggccg ggaaacgccc
     9721 ggacaagcgc ccgaatcttt tgccggcatc agaatcaagc ggtatggctc tgcgcccgac
     9781 ggccggctga ttgtgactcg ccgccatgct ccgttgcggc cgccggcggc ctccatcaca
     9841 gcttcccgga attccgcgtt ttgccggacg gaagctgcta cgatcactcc atcttcgaga
     9901 gaaagaccat cccgcaaatc ggcgatctcg cgcgccgccg cgttcgcgta aagcacgcgg
     9961 ccttcgcggt ctgtcagaaa gaatgcgtgg gagcaccgat ccaggtgacc ggccaacagc
    10021 gagagctgga gccgcagcga agccagctcc ccatgcagca gaacggcccg cttgaggtgc
    10081 ggcatgagcg cgcgtaagac cgctttttcc ggttctccaa aggggccctc gcaggcgccg
    10141 cgcacagccg atatgatgga ttcccgcccc tcagtcgcca gaatagttcc acccattccg
    10201 tgaatggcgc ctcgaggcgc ataaaactcg cggaaggcgg cgctcggttc catctcttcc
    10261 cgccgggaga gatcggcgcc ggctcttacg gcgccttctg gcccgcgcgc gttggcgatc
    10321 cgccacggat ctgcggcgga gtacgactca aaatacaaag tcatatcttc gtcggaccat
    10381 ccataccaac agttcattga aagccggcca tccgcggcgt cgcgcaaagc cagagagcat
    10441 gcctggccgc gaatggaggt gaggaaagcc tccaaaaaca ccttccagca cgatgaatcg
    10501 gccaccgacg cataaatcag accggtcagt tcaagaacgg tatcagcgta aacctggcgc
    10561 ccggccatat gcgaagcccc tcaaacctga gcggattaag gtcccaggga ttctaccgca
    10621 attcgctcgt tcagtgtctg ggttatacaa agatgggcaa gattcgctag taatgcccgc
    10681 ccgggcgtgt tcgggctgcg cttggcggca tccacaaacc gtgaagcgag acctgaaaga
    10741 taggtctccc cccggcgagt gagccaagcg agcggcctac cctttccccg ccgaggccaa
    10801 atcccgcttg cgcttgatct cgatattgag gcgtttgatt ttctggttga gtgtggaaag
    10861 gggaatgcgg aagctctccg ccgtatccgt ctggctccag ttgtacttct cgagctgctc
    10921 gataatgatg cggcgctcga attcatccac caggtcgaaa agcgatgctt ccggaggccg
    10981 cgtaaaggtt ggaatccggc ggcggatcgg aacgccgttg atagtaagca gagtatcggg
    11041 aagcaaatcg accgggaccg tggtatccgt ggccagcaca acggcccgct ctacgacatt
    11101 ctcgagctcc cggacgttgc ccggccaatg gtaatccatc agcatccgca tggcttcggg
    11161 ggcgaagcgc agccgcgagg agccatccgg atcgagaaat ttgtcgtttt cccggcagta
    11221 cttcgtgaag aaatgctcga ccaaggccgg aatgtcctcc cgccgctccc gcaacggcgg
    11281 cagatcgatc tggattacgt tcagccggta atacaaatcc tcgcggaaac ggccttcttc
    11341 aacctgtttg cgcagatcga cattcgtggc cgccacaata cggacatcga ccttgcgcgg
    11401 atcgacgcct cccacgggaa caaactcctt ttcctggatc acgcgcagca gcttggcctg
    11461 catctccagg ctgatcgtgc tgatttcgtc aagaaaaatc gtgccctcgt cggcgatctc
    11521 gaagtagcct ttcttggtct gaatggcgct ggtaaaagat cctttcaagt ggccgaacag
    11581 cgtggattcc agcagctcgg aaggcagcga gccgctgttg acggcgacaa aagccttctc
    11641 ggcgcgcggc gagtttgtgt ggatggcctt ggccatcagt tccttgcccg ttccgctttc
    11701 gccatgcagc agaacggtcg agcggctctg cgccacctgc ccgaccagat cgagcacttt
    11761 gcgcatggcc tcgctcttgc caatgatatt cgagaagttg tagcgctgtt tcagagcgcg
    11821 cttgagctgc gtgttttccc gctcaaggcg gcgcttggag atggtctggt cgatctcgat
    11881 gagcagcttc tcgttgttcc agggtttggt gatgaagtcg ttggcgccca gcttgatggc
    11941 cgccacggcg ttctccaccg aaccatacgc cgtaatgacc atcacggggg tctcgacgtc
    12001 gcgctgccgc agctctttga gcacatccag gcccgagcgg tcgggcatca tcaggtccag
    12061 cagcaccaga tcgtaggctt tggactccag agccagcaaa cccgatgttc cgtcgacggc
    12121 caaatcgacc tggtagccct cgaaggcgag gaacgtctcg aggctttccc ggatcgctgt
    12181 gtcgtcgtcc acgatcagaa tttttccctt ggtttcaggc atgaacttct ttcctcgcga
    12241 gcgggaactc caggcggaag taagttcccc gccccggctt gctctctacc gagatagccc
    12301 ccgagtgctc ctgaatgatg ccgtaggtca ccgccagacc cagccctgtt cccttcttct
    12361 gcccgccctt ggtggtgaag aacggatcgt agattcggtt caggtgctcg ggggcgatcc
    12421 cttctcccgt atcgatgacc tccgcgcgca caacctctcg gcccgagttt cctccggcgc
    12481 ttccggccgc cgtgcggacg gtaagaacgc cgccggcggg cattgcgtcg cgcgcgttta
    12541 agaacaggtt caaaaacacc tgttggagct tgccggtgtt gccctcgatc tccggcaacc
    12601 cttcttcgag ctgagcatca acgcgaatgc cggctttgcg gaactggtgc tccagcaggg
    12661 cgcaagtgtc aagaatcaca cggttcaggc tgactcgcga gaaatcggtc gctgaagtgc
    12721 gggaaaagtt gagcagggag ttgacaatct cgctcgcccg aaacgtctgg gaggcgattt
    12781 tatccagcag gcgcgccttc atctcatcgt gcgacacctg cttggcaagc atttgggcat
    12841 aggtcgagat cactgccaga ggcgtattca cttcatgggc cacgcccgcc gccagcaagc
    12901 cgatagaact gagcttctcc gcctgctgca attgctgctc gagctcgatg cggtcagtca
    12961 catcatcgaa aatcaccagc cggccgatac gatcgaagtt cttcgctacc agcggcgcga
    13021 tggcgatgtt caacgtgcgg tctttggccg gctcggctgg aggcttgggc agcgtctcgg
    13081 gccgcagccg gtatttgtaa atgttgtgaa tgccggtctc ctcgcggacc cgcacaaact
    13141 ctttcacgag atcctcgggg aacagagcgc tcagcggccg ccccacggcc tcgtcgcgcg
    13201 aaatgccgta catcagctcg agctgcgtgt tccagctctc gaccttgtct tccaggtcca
    13261 cggccaggat tcccacgtga atcgactcga cgatgttttc actgaaatcc ttcaaccgct
    13321 cgtactgatc taccttctgc tggagagact ggtacagccg cgcgttctcg atcgcaattc
    13381 ccagatagcc gaccatcgtc tggagcaact ccagatcctc gcccgtcaga aactcccggt
    13441 tgcacgttct tcccaagccg aggaaggcga ctgtttttcc gcgtgcggtg caggggaggt
    13501 aataggtaag atcgagatct gcaatcgtgc tccgtaccgg cgcggtgttg tccaaagccc
    13561 agcgcgtgct ctcgaaaaac agatagggct tcgcctgggc gcttttctcg ggccgggcgg
    13621 cttccagaaa gctcaggtcg agaaaatctc ccggctggat gtagcgcccc ttgcggtccc
    13681 gaagattcag cccttttgcc agttcccaac cggggacgga cgcgcttccg ccggccccgg
    13741 cggcagcagt cgtctcgcgg ggcaaaaaga cggccacgcg ctcgatgccc agcgtctgtg
    13801 aaattctctc ggtgaccgag ccgagcatcg cggcgaggtt tgtctcggca tttagttccc
    13861 gggcgaactc gaccagggtc cgccgataat catagcgctc acggtaaaag aaacggtcca
    13921 gacgttcctg aatccggctt cgcagaggct gaaacaaaaa ggcagccgcg agcatggcga
    13981 cgatccagcc cgtttgcccc aggtcgcgga aattccgctg aattacatcg ccgaggctaa
    14041 aaagcaaagc atagaaggcc ccgagcagcg ccatggtcgc cagcgagtac gcgaagccac
    14101 gccggaaaat tacgtccaca tccatcagcc gatagcgcac gatggcgtag gcgaaggtaa
    14161 gcgggatcag gcccagcggc agcacgccca ggcccatcca agcctgcggc gcggctccaa
    14221 gcatgtaagg gaccgcatag acggccagaa agggcgcaac gccccaaaat gttccgcgct
    14281 tgagccactt gagctgctgc cggaccgtgg atgcctcgcc tgcggatgcc cggcggtaag
    14341 ccgcctcgag cgccagcacg cctgccagga agcacccaga gagcagagcg aaagaaatac
    14401 ggtcgagcag ctcccgtagc tcgaccagag gcagcgaagt ccgcactgcg cctgtggccg
    14461 ccccgacgta agccgcaaaa acgatgacgg ccggcgcata gcaagccgcc accgccgcct
    14521 tccggcgctt cgaccttatc ccgttgtccg aaaaggtcag gcagaagtgc aggaaaaggg
    14581 caggcgcgaa aaggaaggcc cacacgtttg accagtacat cacgcggtca aaggcgttca
    14641 gcttgccggt gtagtggaaa cagtaaagca ggaatgaagc cagacagtat atgtaaaagt
    14701 gctgggcctt tggcgcgccc tcccggcgat agaacacgta taggccgatc agcagataga
    14761 tcagccccag gccgtagagg taatagagaa tcgggctgcg cttcgccggc tcgatgatca
    14821 gcgtgctgtt gagttccacg cctcgccgca tgaaccggta ctcggctttt ttccaagttc
    14881 caatccccag caggaattgc ggtacccgga gcgcttgccg cacagggacg ccgttgatgg
    14941 ccagaagcag atctccgctc ttcagaccgg ccctatccgc gggcccgtcg ggggtgacgc
    15001 ggagagcttg cacaccagac tgcccatcca cccacgttac gccatcctcg ggaagctcga
    15061 accggcgctg ctgttgaaaa ttcagaatgg cggccaaccc ggccgccaca gtcaacaaag
    15121 taagcagcgt tctcgaaaac cggaattgca aatcgtggat cattgtcccc gtcggggcgc
    15181 gcgcacacac acagcgccct tttgagcggc ctgggacgtg cacgccaagt gccactttgg
    15241 ggaggcttac ccgctctgca accggcagca tacaaaccac ttaaattata gtatcccacc
    15301 gccgctagac ttatacgtgt tttggaacaa gaaatacaaa aaaggggatc gacgatgctg
    15361 gccagcgacc c
//
LOCUS       DASREK010000233        21761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1270216, whole genome shotgun sequence.
ACCESSION   DASREK010000233 DASREK010000000
VERSION     DASREK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 21761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21761
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270216"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VFA54_12170"
     CDS             complement(<1..1352)
                     /locus_tag="VFA54_12170"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HZS51613.1"
                     /translation="MDRERIFKELERRNAAALNGGGPERRERQHREGKLSARERLDLL
                     LDPGSFEELDRLVTHRCHDFGMDQQVPPGDGFVTGYGRIDGRLVYVFSHDFTVFGGSL
                     SISNAQKICKVMDLAMKNGAPIIGLNDSGGARIQEGVASLAGYADIFLRNTLASGVVP
                     QISAIMGPCAGGAVYSPAITDFVLMVEKTSYMFVTGPDVIRTVTHEEVSKEALGGAAT
                     HNQISGVGHFIARDDAECLQLIRELLSFLPSNNLDDPPRRSCRDPVDRIEPGLDALVP
                     VESDKPYDMRDIIHAVVDDGYFFEVQQHFAQNLVVGFARLDGRPVGIVANQPAVLAGC
                     LDINSSVKGARFVRFCDAFNIPLVTLEDVPGFLPGTQQEYGGIIRHGAKLLYAFAEAT
                     VPKITVIVRKAYGGAYCVMGSKHIRADINYAFPTAEIAVMGPEGAVNIIYRRELSQAG
                     P"
     gene            1554..1943
                     /locus_tag="VFA54_12175"
     CDS             1554..1943
                     /locus_tag="VFA54_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZS51614.1"
                     /translation="MNAWSEAELKKYELKLREWRHLIEKQARDPGPECPPAARDARKL
                     NEAVLEVLWRKISAAQLREIDLALERLQTGRFGFCEQCGQPIPNERLEATPWVRRCFP
                     CQSRPAAENSGAPGPSIRPLRWYSPFR"
     gene            complement(1967..2173)
                     /locus_tag="VFA54_12180"
     CDS             complement(1967..2173)
                     /locus_tag="VFA54_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51615.1"
                     /translation="MPHANVKKFRPYCPECKDYFTLMAVVPKGADVESYIRESVAKHD
                     HKAFAESKASHFKVRVGQQKQKKK"
     gene            complement(2260..3558)
                     /locus_tag="VFA54_12185"
     CDS             complement(2260..3558)
                     /locus_tag="VFA54_12185"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZS51616.1"
                     /translation="MRLPVFVALAMAVSAALLTANPPDAKEDLHSDVPKVRAKAAEDL
                     GKDRDSQNIPLLRGLLKDPDRQVRAEAVAAIVNIGTQDSLAPLMEATRDSEPSIQMLA
                     VDGLVNFYYPGYVQTGWTAALKKFGSSVKSRFTETNTLVIDPYIKVSPDVVQALGRVA
                     TGGSSMDSRALAARALGILRARDAVPQLIEALRSKDSEVILESLRALEKIGDKSAGPQ
                     FAFLLRDLSEPVQVAAVDATGQLQNREAMPVLRELVKTSKKANVRRAALVALAKMPDP
                     ADKTLYYTYLADKDRYLRAAAAEGIGRCGDASDVDRLKKAFEAEKSESPRLSMAFALV
                     HLGDLSYIPYLLDALNSTFHRGEARPFLIELARQPQVLAQLYKPLQEGSNDQKKNLAE
                     VVAISGNAQSLPYLDQLSHDPNSEVAQEAIRAAKILRARL"
     gene            complement(3559..4401)
                     /locus_tag="VFA54_12190"
     CDS             complement(3559..4401)
                     /locus_tag="VFA54_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HZS51617.1"
                     /translation="MDHPVLRKIAVAHSPDSDDAFMFYGLATQKIRSPLVTFQHVLDD
                     IESLNRAAIEGRYDMTAISYHAYPYVTENYVLTRAGSSVGDGYGPIVVAQRPHAPEEL
                     KGKRIAIPGTMTTAYLVLKLFQPDFQPLVTPFDKIVSAVQEGSADAGLVIHEGQISFD
                     RLGVHRVVDLGRWWKQQYGLPLPLGANAVLRSLHSDIRQECNAMLRKSIEYALAHREE
                     ALSYAMQFARGLDSGLTEKFVGMYVNHYTLDGGSSVAEAAQKLLDLGHEAGLISRHVR
                     LEFA"
     gene            4637..5011
                     /locus_tag="VFA54_12195"
     CDS             4637..5011
                     /locus_tag="VFA54_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS51618.1"
                     /translation="MKPNNKRQILAVVDDLFFATKIGETAKRAGVNVAFATTEEAVLQ
                     FAAQRPSLIIVDLNLNAAKPLPLIAKLKNHPDLKGTSILAFVSHVQGELKQKAQKAGC
                     DMVLARSSFSQNLPQILKRHAE"
     gene            5426..6913
                     /locus_tag="VFA54_12200"
     CDS             5426..6913
                     /locus_tag="VFA54_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51619.1"
                     /translation="MSQADLSFEGLTGVLDAVVETAKRFRRYEEQYQANEAAFDRLQQ
                     QFTQMLETAGHLHSLTEERLAAVSALMVELKARHSQGEQALGDARDLIEQIKLLQPKL
                     DQDLAALGTRQQVDSERTIQLAHTLDVVQALVGQLQARQGQIEQSLMTLETLLRDQSK
                     RNGDRDGDWNGVRGLAEQVAERQAQCEKALSIIQNTQQAEKGVISHFEKSLNTVRTMA
                     VELEKRVGQVEVSLAAIKANGSDASNINLQSVEAARRLMEDLGERQATLQTLIDQAQQ
                     TAANAAEQVARVDEQVNRIEKTSALALEEAQSAAQKAAQALDKAAQAASSPAPTIVAA
                     PDDPAQAATDFQGFLQRCQEDHQAALDRNNQLQAQMRAALKDWPAMGESAIQKFFEQG
                     RARLEQIVNNWFQHREQRVTEMESRFDEMLEKTVALHQQLEKSSGAAAPAAVPPEWME
                     SLEAVSAAHGSELRFLKTLLWITLAAVALSYGLIVYAMILRSPAS"
     gene            complement(7000..8676)
                     /locus_tag="VFA54_12205"
     CDS             complement(7000..8676)
                     /locus_tag="VFA54_12205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS51620.1"
                     /translation="MPSIFTDGDARPSWPVIACLAALIFTFAFRCCRTHRVFNDTADE
                     AVHIACGLEVIEKHQYTLEPQHPPLGRVLLAVPAHLAGLRSKPHAPLWRGESSEFYWR
                     TLSMARVGTLFWAPFLILYIYRWARQLYGRAAGIGAAALAAFSPNLMAHASLATLDFG
                     AAASIFISLYYFWRWSQRPGAGPCLAAALAFAIAVLVKFSALALIPPVALAFFLIGRW
                     SKIASILGESRSGRLWKSLQAGLGRWALFLITTALIVWGGYGFDVGPLGRPAFRPAMG
                     AMAHALEHKAEIVAGRRALPAPRFWRGILDVAGHNAEGHPCYLLGRVSLFGWWYYFPV
                     ALAVKTTIPLLLMVSLSTAACLAAFRSGGYRRAVYPLAAAIIILVICMKSSLNLGIRH
                     ILPIYPFFALLAAGLFADRSFRRPRLGKALAGIGIALAGWHSAESLLASPDYLAYFNQ
                     AARGREEKFLLDSNLDWGQDLERLRRFLAEKRISTIYLSYFGAADPASRGIVGARALP
                     AGLRPSGWVAVSKNHIAGLALEHDYRWLTAYTPAARIGKSILVYYFPSPP"
     gene            complement(8657..9307)
                     /locus_tag="VFA54_12210"
     CDS             complement(8657..9307)
                     /locus_tag="VFA54_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51621.1"
                     /translation="MVDKLLESIWKAGLILLGRQVYEQYALSTPIFIDLRHRLYEDVR
                     LIEQIGCALHRKICEIVGGVSPAPQQILGIPDTATPLALAAAMASLGTGLPLIYGQMR
                     KRPAAYPGGVYGVSSFMGTYRPDREITLVDDVMASGRSKLWAIEELAKNGIHVTRILV
                     VVDREQGGNEILRERGYPVYCLYTISQMLEYFVQTGKIEPAAAREALDHLRAKHFH"
     gene            complement(9389..11023)
                     /locus_tag="VFA54_12215"
     CDS             complement(9389..11023)
                     /locus_tag="VFA54_12215"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HZS51622.1"
                     /translation="MFLPLTPLRFLKRAAWEYGPKIGVVCAEQRFTYSQFDARVRRLS
                     TALKMGGIRPGDRVAFLSFNCHRLLEGYYAVPQAGAIILPLNVRLSADEQAAILQHSG
                     ARAVFFAPEFLPVVEQFRDRVKGIEWYVALDSGTALGGPASPRSLPPARWIDSRTYEQ
                     LLAEAEPDQRDYTAVDENAIAELFYTSGSTGQPKGVMLSHRTLYLHAFYVLASLPQLD
                     DAVELHTIPLFHANGWGRPQTITLAGGRHVMAKRFDPSWVLETIEREKVTGFSMVPTM
                     AAALVNCPDLGKYDVSSLRHIFLGGAASSPALVRAVERALGCRCYVGYGLTETSPVLS
                     EARIKSTLAGLTEEQRIERQAMTGYAIPGVEMRVCDAEGRDVPRDMAHVGEILARSDV
                     VMDGYWNDAEATRGAISDGWLRTGDMAVWDEHGYVLIVDRKKDIIISGGENISSIEVE
                     KVLAAHPAVYECAVVGVPDPKWGEAPKAFVSLKPDVPAPTEEALKEFVRSRLAGFKVP
                     KSIEFLDALPKGATGKILKRALREPYWAGMDKRVHG"
     gene            11239..12321
                     /locus_tag="VFA54_12220"
     CDS             11239..12321
                     /locus_tag="VFA54_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51623.1"
                     /translation="MWRRGLAAGLLAVGLAMAQGGLTLQQLTQLIQSSVKLKQSDKEV
                     ASYLRKQKLSFALTDVAIEELQGMGAGPQTVAALRELQKASRGLPPPPKVSEVAQPAK
                     PVEPPPSPEEQKRIIEQARANALSYTKRLPDFICLQLTRRYVDPTGLELDWRKYDEIK
                     ARLTYFEQKEDYKVISVNEQLTTRSFESLGGTTSQGEFGSMLAELFAPETAAEFEWDH
                     HSVLRDRKVYVFRTHVPKSRSQWHISFQRQREVIAGYHGLVYIDKDNLSVLRLSLVTD
                     ELPSDFPITEARTALDYGYAKIADRDYLLPLHATVRLRESKFLTRNEVEFRLYRKFST
                     EATITFDDEAKGVQPEETKPPAEPQN"
     gene            12476..13297
                     /locus_tag="VFA54_12225"
     CDS             12476..13297
                     /locus_tag="VFA54_12225"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZS51624.1"
                     /translation="MRVACPILAGFAAAGVLLAVTAGCQQKRARQAARPLIDPDRAID
                     LTYPLDDSNIYWPNAKPFHYQRDAGGPTPGGYWCAAGSFSASEHGGTHIDSPVHFAEG
                     KATVDEILLGRLIAPAVLIDITAACAQNRDYELRIEDITEWERIHGRIPDGAAVLVRT
                     GWSKYWPDKKQYLGSDAPGDVSHLHFPGISQNAAEFLAGMRKIYGVGIDTASLDPGTS
                     KTFMAHRILAGARIYGLENVANLDKVPAAGATLIALPMKIRGGTGGPTRIIAILP"
     gene            complement(13386..14471)
                     /gene="wecB"
                     /locus_tag="VFA54_12230"
     CDS             complement(13386..14471)
                     /gene="wecB"
                     /locus_tag="VFA54_12230"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HZS51625.1"
                     /translation="MKLACIAGARPNFVKMAALVAEIRRRPGLQYQLIHTGQHYSAEM
                     SASFFSDLNIPPPDVNLDVGSGTSLWQTAEIMKRLEPVLNQSRPDVILVVGDVNSTLA
                     GALAGVKLGIPVAHVEAGLRSFDRSMPEEINRVLTDAISDFLFVSEPSGEKNLLAEGV
                     PREKIFFSGNVMIDTLLRFQKKAEMSDVLARLGLRAGEYAVATLHRPSNVDRADALAG
                     LMKALTEIARRLPVIFPVHPRTEQMLKTLQPGSDGVVLISPLGYLDFLHLVSHACLVL
                     TDSGGIQEETTILQVPCLTLRTNTERPVTIAEGTNILVGGDPAHIVEAAIAALESPRG
                     RSRVPERWDGRASERILDVLTATCARR"
     gene            complement(14468..15415)
                     /locus_tag="VFA54_12235"
     CDS             complement(14468..15415)
                     /locus_tag="VFA54_12235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS51626.1"
                     /translation="MRVASEGYECAECARLYPFVFGIPDFRISLPAHFDLSRDTETAR
                     VLVESEKDLDFEGLLHLYYRLNPEPGEDLHDKHMAHLAGEEDQARAALELIEESREFL
                     PGEAVLEVGCGVGQYLAVAAERADHVAGVDLCLPFLVLTRKRLGPQALLAAADAGRLP
                     FRDGAFAAVIAADVIEHLADPQQSLAEMGRVLVAGGPLFLSTPNRFSLSPEPHVGLWG
                     VGYLPRRLANRYVLRRRRICYDDVRLLSARSLSRMLHAHFPGRARLLIPALSPRQLQH
                     FPPLKRRLAEAYLALRRVPLLRGVFYCFGPFFQVVAEKR"
     gene            15490..16557
                     /locus_tag="VFA54_12240"
     CDS             15490..16557
                     /locus_tag="VFA54_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS51627.1"
                     /translation="MLRTAILGLGGVAERIHIPACLAAPGIELAGAAEPVEEVRRRMA
                     QKFRLPAVYESAETLLEKEKPDLVIIGAPPDSHRDLCLLSFSHGAHVFCEKPFMRTLA
                     EADEVIAAARSHGRLLAVNNQYRYMPIYSRTRERLDRGDFGRLYLIQCWQQMFHPPAV
                     EKVAWRAALKQSTLYEFGTHALDLMCYLFGSLPVALTAQIPAAHPAYTSDVVVLMTLR
                     FPAERAATLVMNRISRAPERYLEMRLDCERASLRLSLGGVARASVEWSKRLGRPTGRF
                     SFVKGGEAREESGGRSSAYVQEKSPAFMPATAAHLRRFAAEIESGSRNYEPALHAREV
                     LALALAGYEAAASGETIWFQR"
     gene            16586..16894
                     /gene="nirD"
                     /locus_tag="VFA54_12245"
     CDS             16586..16894
                     /gene="nirD"
                     /locus_tag="VFA54_12245"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HZS51628.1"
                     /translation="MSAFVKVAKASDIPAGSGKVVEAGGKQIAIFEAGGKYYAIDNTC
                     THRGAPLAEGVIQGTEIECPWHRAHFNLETGQATRPPAARSVGVYPVRQQGDDIEIEI
                     "
     gene            16977..17438
                     /locus_tag="VFA54_12250"
     CDS             16977..17438
                     /locus_tag="VFA54_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZS51629.1"
                     /translation="MPPRILDGISQLRSLAGQEAGASGWLEVTQSLVDEFAAATGDRQ
                     WIHVDPARAQAESPFGRTIAHGFLTLSLLSRLASQAIEVRGFRMSINYGLNRVRFPAP
                     VPAGSRIRAHFTLQSIEELARCAQATWAVIVEVEGAAKPSLVAEWVVRYYQ"
     gene            17512..18075
                     /locus_tag="VFA54_12255"
     CDS             17512..18075
                     /locus_tag="VFA54_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS51630.1"
                     /translation="MEKQEKLARFEQTVMPHLRAAYNLARWLARDAQDAEDIVQEAFV
                     RAFGFFEGFHGQDARAWLLAIVRNTGYTWLRQNRSRQLETAFDEEVHSPEAGSLNAEA
                     SLLREADSRLVQDALEALPRESREVLVLRELEGLSYKQISEVAGVPLGTVMSRLARAR
                     QALQARLGRKPSGQGRAKKEADELQRD"
     gene            18056..18838
                     /locus_tag="VFA54_12260"
     CDS             18056..18838
                     /locus_tag="VFA54_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZS51631.1"
                     /translation="MNCSEIEELIHAYSDGELDLIRSLEIEQHLRTCAACSRSHQELE
                     ALRSAMRAAPLYFELPKQLQGQIHSALEKTGGEQTMRTPAKRRAAWLPWLRGAAAAAL
                     IAVIFGALAPLFSRRPADDLLAQEVVASHVRSLMPGHLTDVPSTDEHTVKPWFNGKLD
                     FSPTVKDLAAQGFTLAGGRLDYLQGRPVAALVYQRRRHYVNVFVWPSARSTGGEERPS
                     SRQGYNLVHWTQSGMTYWAVSDLNSGELQEFSRLLQTGGSLR"
     gene            19012..20232
                     /locus_tag="VFA54_12265"
     CDS             19012..20232
                     /locus_tag="VFA54_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZS51632.1"
                     /translation="MAKRIVYDHNPDGIDRRGFLECMAWAGTGILWTITAGVPKSYAL
                     PQADHKTSHRKDGLSFMQISDSHIGFSKEANKDVITTLQAAVSKINAAPEPPAFLIHT
                     GDITHLSKPEEFDIAEEVLKGAKANRVFYVPGEHDVLNDNGKQYLERYGKETRGGGWY
                     SFTKQGVHFIGLVNVLDLKAGGLGQLGGDQLEWLKRDLAGLSNSTPVVVFAHIPLWTV
                     YPDWGWGTADSEQALAYLKRFGSVTILNGHIHQTLQKVEGNVVFHTAMSTAFPQPAPG
                     AAPSPGPMKVPAEKLRSVLGVTSVKYVPGRRSLAIMDSPLEAAPVSGPAAAQIAIDNF
                     SFIPAEIKVPAGGTVAWTNHDDVPHNVISTEKRFASPVLDTDEKFSYTFQNPGVYPYY
                     CSIHPKMTAKVIVE"
     gene            complement(20283..21407)
                     /locus_tag="VFA54_12270"
     CDS             complement(20283..21407)
                     /locus_tag="VFA54_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZS51633.1"
                     /translation="MSTKQEIARYRANLQDEIDSVSLYNTLARCERQPQLAEVYRRLA
                     AVEERHAHFWEQKLREAGVVMKRRKPGWRSRLLGWLAGRFGPQFVLPTISAMEQADRH
                     DYLLQPETKPSGMPAEEESHARLLRTIAGPRGIEGGVLAQLEGRHRAIGGNALRASVL
                     GVNDGLVSNLSLVMGVAGANLSNRAILITGLAGLLAGACSMALGEWLSVTSSRELYER
                     QIGVEAEELNAVPDEEAEELALIYQSKGLSEGEARSVANRLVSDKATALDTLAREELG
                     IDPETLGGSAWEAAVMSFVLFVTGAILPVAPFMFLSGYPAVLGSLVMSSLGLFGSGAA
                     ITLLTGRTILYSGVRQLIIGLGAAALTFGIGKILGVTVAG"
     gene            complement(21411..>21761)
                     /locus_tag="VFA54_12275"
     CDS             complement(21411..>21761)
                     /locus_tag="VFA54_12275"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZS51634.1"
                     /translation="VGAPEGRFEDAVRRANAYREAGADCLFVPGVRDATLIRRLAQEI
                     RGPINILAGPGVPPAPELERLGVARVSSGSGPMRATLALIRQIAGELKERGTFASFTG
                     NAIPYAEANRLFGG"
BASE COUNT         4484 a         6754 c         6575 g         3948 t
ORIGIN      
        1 gggccggcct gcgacagttc ccggcgataa atgatattca ccgctccttc cggccccatc
       61 acggcgattt ccgcagtggg aaaagcgtag ttgatatccg cccggatgtg tttcgatccc
      121 atcacacaat aagctccgcc gtaggccttc cggacgatca cggtaatctt gggcactgtc
      181 gcttcggcga aagcatacag gagcttcgcg ccgtgccgga tgatcccgcc gtattcctgc
      241 tgcgtgccgg ggaggaaacc cggcacatct tccagcgtca cgagcgggat attgaacgcg
      301 tcgcagaacc gaacaaaccg cgcgcctttc accgaagaat tgatgtcgag gcaaccggcc
      361 agcaccgcgg gctgattggc gacgattcct acgggccttc catccagccg ggcgaagccg
      421 accacgagat tttgggcgaa atgctgctgg acttcaaaga agtatccgtc gtccaccacg
      481 gcgtggatga tgtcgcgcat gtcatagggc ttgtcggact cgaccggcac caaagcgtcg
      541 aggcccggct cgatgcggtc gaccggatcg cggcaggatc gcctgggagg gtcgtccagg
      601 ttgttggagg ggagaaagct cagcagttca cgaatgagct gcaaacactc cgcgtcatcc
      661 cgcgcgatga aatgaccgac gccgctgatc tggttgtggg tggccgctcc gccgagagct
      721 tccttgctaa cctcctcgtg agtcacggtt ctgataacgt ccggaccggt aacaaacatg
      781 tagctggtct tttcgaccat cagaacgaag tcggtgatcg cgggcgagta gaccgcgccg
      841 ccggcgcaag gtcccatgat ggccgagatt tgcggcacca caccactggc caaggtgttc
      901 cttaaaaaga tatcggcgta accggccaga gaggcgacgc cttcctgaat ccgggcgccg
      961 cccgaatcgt tcagcccgat gatgggagcg ccatttttca tggccaaatc catcactttg
     1021 cagatcttct gcgcattgct gatggaaagc gagccgccaa agacggtgaa gtcgtgcgag
     1081 aagacgtaaa ccaaacgacc gtcaatgcgg ccatagccgg tcacaaaacc gtcgccgggc
     1141 gggacctgct gatccatgcc gaaatcgtgg caccggtggg ttacgagacg gtcgagttcc
     1201 tcaaagctgc ccggatccag cagcagatcg aggcgctcgc gggccgaaag tttgccttcc
     1261 cggtgctggc gctcccgccg ttcgggaccc ccgccgttca aagcggcggc gttgcggcgc
     1321 tcgagttcct tgaaaatccg ttcccgatcc atagatgtgg cccgcgaggt cctttccact
     1381 ctttatttct tcggggcaag caaaatcgca agctggcggt cgatccgcca acggttctag
     1441 cctaacaaac aggaacgggc cgcaaaaggc aaagagtact gagacctttt tgtgcggtaa
     1501 ctaagggaat tcaacgactg ctccgggatt ggctccatgc taacaacgat agggtgaacg
     1561 cgtggagcga ggcggaactg aaaaaatacg aattgaaatt gcgggaatgg cggcatctga
     1621 ttgaaaaaca ggctcgggat cccggaccgg aatgcccgcc ggcggcccga gatgcacgca
     1681 agctgaacga ggcagtgctg gaggttctct ggcgaaaaat ctcagcggcg cagctccgcg
     1741 agatcgatct tgctctcgag cgactgcaaa ccggcagatt cggtttctgc gagcaatgcg
     1801 gccagcccat tcccaacgaa cggctggaag caacgccctg ggtgcggcgt tgctttccct
     1861 gccagagccg cccggcagcg gaaaattccg gcgcgccggg cccctctatc aggccgctcc
     1921 gatggtattc cccttttcga taaaaaagtc ggtgagcgaa acgctgttac ttcttctttt
     1981 gtttctgctg cccaacgcgc accttgaagt gtgaggcttt cgactccgca aacgctttgt
     2041 gatcgtgctt ggctacggat tcgcgaatat agctttccac atccgcgccc ttgggtacaa
     2101 ccgccattaa ggtaaaataa tccttgcact ccgggcagta gggacgaaac ttcttcacat
     2161 ttgcatgtgg catgctggcg gaatcctcca gatcaggtca ataaatatta attgtagcgc
     2221 aacccgaggt attctgccac tccggatggt ggtaaggggt taaagtcgcg ccctcaatat
     2281 cttagcagcc cggatggcct cctgggccac ctcggaattg gggtcgtggc tgagctgatc
     2341 gaggtagggc aggctttgcg cgttgccact aatcgcgacg acttcggcta agttcttttt
     2401 ctggtcgttg ctgccctctt gcagcggctt gtaaagctgg gccagcacct gaggctgccg
     2461 ggcgagctca atcaagaagg gccgggcttc gccccggtga aacgtcgaat tcagcgcatc
     2521 caacaggtat ggaatgtaac tgagatcgcc caagtggacc aaagcgaagg ccatagagag
     2581 gcggggagat tcgctttttt ccgcttcgaa ggcctttttc agccgatcca cgtcgctcgc
     2641 atcgccgcac cggccgatcc cttcggccgc ggcggcccgc aaataccgat ccttatcggc
     2701 aaggtaggtg taatacagcg tcttatccgc aggatcgggc attttggcga gcgcaactag
     2761 agcggcccgg cggacattgg ccttcttgct ggttttcacc agttcgcgga gcacaggcat
     2821 ggcctcgcga ttttggagct gccccgtcgc atcgaccgcc gccacttgca ccggctcgct
     2881 caaatcccgg agcaaaaagg caaactgcgg gccagcggat ttatcgccga tcttctcgag
     2941 cgcccgtaga gattcgagaa tcacctcgct gtccttggag cgcagagcct cgatcaactg
     3001 cggcacagcg tcccgagccc gcaagattcc caaggcgcgc gccgcgagcg cgcgggaatc
     3061 catactcgat ccccctgtcg ccacgcgccc gagggcttgc acgacgtccg gggacacctt
     3121 gatataagga tcaatcacca gcgtattcgt ttcggtaaag cggctcttga cgctggaacc
     3181 gaatttcttc agagccgctg tccagcccgt ttgcacgtag cccgggtaat aaaagttgac
     3241 cagtccatcc acggcgagca tctgaatgga cggctccgag tcgcgcgtcg cttccatcag
     3301 cggagccagg ctgtcttgtg tgccgatatt tacaatggcc gcgacagcct cggcgcgcac
     3361 ctgccggtcg gggtctttca ggaggccgcg caaaagcggg atgttctggc tgtctctgtc
     3421 tttgcccaga tcctcggcgg ctttcgcccg aaccttggga acgtcgctgt gcaaatcctc
     3481 cttggcgtcc ggcgggttgg ccgtcaacag cgcggcgcta accgccattg cgagcgcgac
     3541 gaaaacaggt aaccgcattt atgcaaactc cagccgaacg tggcgggaaa taagccccgc
     3601 ctcatgcccg aggtcgagca gtttttgcgc ggcctcggcg acggacgatc ccccgtcgag
     3661 ggtatagtga ttgacgtaca tgcccacgaa cttctcagtc agcccggagt cgagcccccg
     3721 ggcgaactgc attgcatagg aaagcgcctc ctcccggtgg gccagggcgt attcgatgct
     3781 tttgcgaagc attgcgttgc attcctggcg aatatcggaa tggagggagc gcaaaacagc
     3841 attcgcgcca agcggcagcg gcaacccata ttgctgcttc caccagcggc caagatccac
     3901 gacgcggtgc acaccgagac gatcgaagct gatctggccc tcgtgaatca ccaggcctgc
     3961 gtccgccgag ccttcctgca cagcgctcac gatcttatcg aacggcgtca ccagcggctg
     4021 gaagtcgggc tggaacagtt tcagcaccaa atacgctgtg gtcatcgttc cgggtatggc
     4081 gatccgcttg cctttcagct cttccggggc gtgcgggcgc tgcgccacca cgataggacc
     4141 gtacccgtcg cccacgcttg agccggctcg cgtcaataca tagttctcag tcacataggg
     4201 atacgcgtga tacgagatgg cggtcatatc atagcggcct tcgatcgcgg cccggttgag
     4261 gctttcgatg tcgtcgagca cgtgctgaaa agtgaccagc ggggagcgta tcttctgagt
     4321 cgcaaggccg tagaacatga aagcatcatc ggaatcaggg ctatgggcga cagcaatttt
     4381 gcgcaagacc gggtggtcca tagactaacg gtagactgtg gcggacggtt caaagctatc
     4441 acatcgccgg ccaaggaggc aactttgaca cgggtcgcaa tctggtttaa gctgatagag
     4501 gttccctgca aggatccctt caccatctcc tgccgttggc tggaacgggc ggtcagtctg
     4561 tccggctccg caggatggaa gaccgttgcc cggcaagccg accagtgcac agttcgcgga
     4621 ctggaaagag cggcctatga aaccgaataa caagcgacag atcctggcgg tcgttgatga
     4681 tctgttcttc gcaaccaaaa ttggagagac ggccaagcgg gcgggagtga acgtcgcctt
     4741 tgccacgacc gaagaagccg tgctgcaatt cgcggcgcag cggccgtcgc tgatcatcgt
     4801 ggatttgaat ctgaacgcgg caaagcccct tcccctgatc gcgaagctga aaaatcatcc
     4861 agatctgaag gggacctcta ttctggcctt tgtctctcac gtgcaaggag aactcaaaca
     4921 gaaagcgcaa aaggcaggct gcgacatggt gctggcccgc tcttcttttt cacagaatct
     4981 tcctcaaatt ttgaagcggc atgccgaata aggggccttg ctcggcgccg ctttttccca
     5041 aaattaacaa caaaatcttt cgccgcatgt aacaaaaagc actccggcaa cacttattac
     5101 cagagataag ttcggcctga gattaccggc ggccgccggt ctcctgcggc agcttggaac
     5161 tctcaagcaa gagcttatca atgagttccg atgcgggtca tattcgtcca tgcgccgcgt
     5221 cggtgacagc ggggagcgcg cctgccatcc ggcgcgctct cccgccccct cccggcagcg
     5281 gcggcacgcc gggcttttac ggctacgccc ttcgcgccca gccttaggtc tctttcctac
     5341 cttctattag gagaagcact gtatggagtt gaacgccccg ggtggctgac actcgatctg
     5401 ggcaaccgcc tgcggaggca cccccttgtc ccaagccgac ctttcttttg aaggactcac
     5461 cggagtgctt gacgccgtcg tcgaaacggc gaagcgcttt cgccgttacg aagagcagta
     5521 tcaggccaac gaagcagcct ttgaccgtct ccagcagcaa tttacacaaa tgctggagac
     5581 cgcggggcat ttgcactccc tgaccgaaga gcgcctggcg gccgtcagcg cccttatggt
     5641 cgagctgaag gcgcgccact cccaagggga gcaggcgctg ggggacgccc gagacctcat
     5701 cgagcagatc aagctcctgc aacccaaact cgatcaggat ctcgcggcgc tgggaacacg
     5761 gcagcaagtg gactccgagc gcacgatcca gttggcgcac accttggacg tggtgcaggc
     5821 gctagtcggg caactgcaag cccggcaggg tcagatcgag cagtctttga tgaccttgga
     5881 aaccctgctg cgcgatcaaa gcaagcgcaa cggagatcgc gacggcgatt ggaacggagt
     5941 ccgaggtctg gcggaacaag tggcggaacg ccaggcccag tgcgaaaagg cgctcagcat
     6001 tattcagaac acccaacagg ccgaaaaagg agtcatctcc catttcgaga agtccctgaa
     6061 cacagttcgc acgatggcgg tggagctgga aaagcgggtg ggacaggtcg aggtttccct
     6121 ggccgccatt aaggccaacg gaagcgacgc cagcaacatc aacttgcaat cggtggaggc
     6181 ggcgcggcgg ctcatggagg atcttgggga gcggcaggcc accttgcaga cgctgatcga
     6241 tcaggcgcag caaaccgcag cgaacgcggc ggaacaagtg gcccgcgtgg acgagcaggt
     6301 gaatcggatc gagaaaacga gcgcactggc cctggaagaa gcgcaatcgg cggcgcaaaa
     6361 agccgcgcag gccttggaca aggcggcgca agcggccagt tccccggcgc cgaccatcgt
     6421 cgcggcgccc gatgatccgg cgcaggccgc cacggacttt caaggatttt tgcagcgctg
     6481 ccaggaagat caccaggctg ctctcgatcg caacaaccag cttcaggcgc aaatgcgggc
     6541 cgccctgaag gattggcccg ccatgggcga gagcgcaatc cagaaatttt tcgagcaggg
     6601 ccgggcccgg ctcgaacaga ttgtcaacaa ctggttccag caccgcgaac agcgcgttac
     6661 cgaaatggaa agccgtttcg atgaaatgct ggaaaagaca gtggcgttgc accagcaact
     6721 ggagaagtcc agcggggccg cggcgccggc cgccgtccct ccggagtgga tggaaagcct
     6781 ggaagcggtg tccgccgccc acggctccga actgcggttt ttgaagaccc ttctctggat
     6841 cactctggcg gcggtcgccc tttcttacgg cctgatagtt tacgccatga ttctccgcag
     6901 cccggcaagc tgaagccggc cctcaggcag cgcgcgccac gcctctatgg gccggcggct
     6961 tccattaaat gcctaaaatt ttcgggaatg ccccaggatt tacggcgggg atggaaagta
     7021 ataaaccaaa atggattttc cgatgcgagc cgcgggcgta taggccgtga gccagcgata
     7081 atcgtgctcc agagccaggc cggcaatgtg atttttgctc acggcaaccc agccgctggg
     7141 ccgcaggcca gcgggcaagg cccgcgcgcc gacgatgccc cgggaagcag ggtcggcggc
     7201 gccgaaatag ctcaagtaga tcgtgctgat tcttttctcc gcgagaaatc tccgcaaacg
     7261 ttccagatcc tgcccccagt ccaaattgct gtcgagcaaa aatttctctt cccgccccct
     7321 cgccgcctga ttgaagtacg ccagataatc cggcgaggcg agcagtgatt cggcgctgtg
     7381 ccagccggcc agagcgatgc cgatgccagc cagcgctttt cccaggcgcg gccggcggaa
     7441 gcttcgatcc gcgaaaagac ccgccgccag caatgcaaaa aagggataga tgggcagaat
     7501 gtgccggatg cccagattga ggctgctctt catgcatatt accaatataa tgatcgccgc
     7561 ggccagggga tacacggccc tccgatagcc cccgctccgg aacgccgcaa ggcaagccgc
     7621 agtgctcagg ctcaccatta gcaacagagg aatcgtcgtt tttaccgcca gcgccacagg
     7681 aaagtagtac caccacccga aaaggctgac acgcccgagc agatagcacg gatgcccttc
     7741 ggcgttatgc ccggccacgt cgagaatgcc tcgccagaag cggggcgcgg gcagagctct
     7801 tcggccggct acgatttcgg ccttgtgctc cagcgcgtgc gccatggcgc ccatggcagg
     7861 ccggaaggca ggccggccca gagggccgac gtcaaacccg tatccgcccc atacgatcag
     7921 agccgtcgtg atcaaaaaca gcgcccagcg gccgaggccc gcctgcaagc tcttccaaag
     7981 cctgcccgat cggctttcgc caaggataga ggcgattttg ctccagcggc cgatcagaaa
     8041 aaacgccagc gcgacgggag gaatcagcgc cagggcggaa aacttaacca gcacggcgat
     8101 ggcaaatgcg agcgccgcgg ccaggcaggg tccggcgccg ggcctctggc tccaacgcca
     8161 gaaataatag agcgaaataa aaatactggc agcggccccg aaatcgagcg tggccagact
     8221 ggcgtgggcc atcagattgg gcgaaaaagc ggccagggcc gcggcgccga tgccggccgc
     8281 gcgcccgtaa agctggcggg cccaacgata gatataaagg atgagaaacg gcgcccaaaa
     8341 cagggttccc acgcgggcca tggatagcgt acgccaatag aactccgaag attcgccgcg
     8401 ccataggggc gcgtgcggct tgctgcgcag ccccgccagg tgcgctggga cagccagaag
     8461 cactcttccc agcgggggat gttgcggctc cagggtgtat tggtgcttct caatcacctc
     8521 gagcccgcag gcaatatgca cggcctcgtc ggctgtatcg ttgaagaccc ggtgagtgcg
     8581 gcagcagcga aaggcgaaag tgaagatcag cgcggccagg caggcaatca cgggccacga
     8641 gggccgcgca tcgccatcag tgaaaatgct tggcacggag atgatcgagc gcctcacgcg
     8701 ccgctgccgg ctcgatcttg ccggtctgga cgaaatactc aagcatctgg ctgatggtgt
     8761 acaagcaata tacgggatag ccccgctccc tgaggatctc attgccgccc tgctcgcggt
     8821 ccaccacgac cagaatccgg gtcacatgga tgccattctt ggccagctcc tcgatagccc
     8881 acagcttgga gcggccggag gccatgacat catccaccag ggtaatctcc cgatcgggac
     8941 gataggtgcc catgaacgag ctcacgccgt acacgccgcc aggatatgca gccggccttt
     9001 tccgcatttg tccgtaaatc aacggcaggc cggttcccag cgaggccatg gcggcggcca
     9061 aagccagcgg agtcgccgta tcgggtattc ccagaatctg ctggggcgcc ggcgagacgc
     9121 cgcccacgat ctcgcaaatc ttccggtgca gggcgcagcc tatctgctcg atcagccgga
     9181 cgtcctcata gagccggtgg cggaggtcta taaaaatcgg agtggacaaa gcatactgct
     9241 catacacctg ccgccccaac aggatcaggc cggctttcca aatggattcg agaagtttat
     9301 caaccatgag gaatcggcgt ttccttgcca gataacctgc gggcggcgaa ggcgctgagt
     9361 ccgcgccgat caaacacgat cgtccctttt atccgtgcac ccttttatcc atcccggccc
     9421 agtagggttc gcgcagcgcc cgtttcagaa tcttgcctgt ggctcctttt ggaagggcgt
     9481 cgagaaactc gatggactta ggaaccttaa agccggccag gcggctgcgc acgaactcct
     9541 tcagcgcctc ctcggtagga gccggcacgt ccggcttgag gctcacaaac gccttgggcg
     9601 cctctcccca tttcggatcc gggacgccta ctacggcgca ttcatacacc gcggggtggg
     9661 ccgccagaac tttctcgacc tcaattgagg aaatattttc gccgccgctg atgatgatgt
     9721 ccttcttgcg atcgacgatc agaacgtaac cgtgctcatc ccagacggcc atatccccgg
     9781 ttcgcagcca tccgtcgctt atggcgccgc gcgtcgcctc ggcgtcgttc cagtagccgt
     9841 ccatgaccac atcgctgcgg gcgaggattt cgcccacgtg agccatatcc cggggaacgt
     9901 cccggccctc ggcgtcgcac acccgcatct cgacgccggg aatcgcgtag ccggtcatcg
     9961 cttgccgctc gatccgctgt tcctcggtga gcccggccaa ggtgcttttg atgcgcgctt
    10021 ccgatagaac gggcgaagtc tcggtcagcc cgtatccaac atagcagcgg cagcccaacg
    10081 cgcgctcgac ggcgcgcacc agcgcgggcg aactggccgc cccaccgagg aaaatgtgcc
    10141 gcaggctgga gacatcgtat tttcccaggt cgggacaatt gacgagcgcg gcagccatcg
    10201 taggcaccat ggaaaaaccc gtcacctttt cgcgctcgat cgtctccagg acccaggagg
    10261 gatcgaagcg cttcgccatg acatgccggc cgccggccag cgtgatggtc tgcggccggc
    10321 cccatccgtt cgcgtggaac aaaggaatcg tgtggagctc caccgcgtcg tcgagctgcg
    10381 gcagactcgc cagcacgtag aaggcgtgca ggtagagcgt gcggtggctc agcatcaccc
    10441 ctttgggctg ccccgtggag ccgctggtgt agaagagctc cgcaatggca ttttcatcta
    10501 ccgccgtata gtccctctgg tccggctccg cttcggcgag gagctgttca tatgtgcggg
    10561 aatcgatcca gcgggccggc ggcagcgaac ggggagacgc cgggccaccg agggctgtgc
    10621 cagaatccaa tgcgacgtac cactcgatgc cttttacccg atcgcggaat tgctccacca
    10681 cggggagaaa ctccggagcg aaaaacacgg cgcgcgcccc ggaatgctgt aagatcgcgg
    10741 cctgctcatc cgccgaaagc cgtacattga gcggcaaaat gatggctcct gcctgcggga
    10801 ctgcgtagta gccctccagg agccggtggc agttgaaact caaaaaagct acccggtcgc
    10861 ccggcctgat gccgcccatt ttcagtgcgg tgctgagccg gcgcacacgc gcatcgaact
    10921 gggaataggt gaagcgctgt tccgcgcaaa ccactccgat cttcggtccg tattcccaag
    10981 ccgcgcgctt caggaagcgc aacggagtca gcggtaagaa catgacccgg agcccagctt
    11041 attgtacatg atggcaattc cggcctatgc ctgcaagagc gcgccgcttc gccgccaggg
    11101 aactcagatt ttagggaacc cgctgttttc ttccgccgac tccagcccgg cgggaggcag
    11161 cgccgggaaa gaaggggtcg ccgggcgagg cttcggagtc tgtgtgggac cgcaaggcgg
    11221 cggtagtaaa atagggtcat gtggcgccgg ggtctcgcgg ctggcttact ggcggttggg
    11281 cttgcaatgg cccagggcgg cctcacactg caacagctaa cccaactgat ccagtcctcg
    11341 gtaaaactga aacagagcga taaggaggtc gcgtcgtatc tccgcaagca gaagctcagt
    11401 ttcgcgctga ccgacgtagc cattgaagaa ttgcagggta tgggcgcagg tccgcagacg
    11461 gttgctgcgc tgcgcgaact gcaaaaagcc tcgcgggggc tgccccctcc ccctaaagtg
    11521 agcgaggttg cacagcccgc caagccggtc gagccgcctc cttcgccgga agaacaaaag
    11581 cgaatcatcg aacaggctcg cgccaacgct ctgtcttaca ccaagcgcct gccggacttt
    11641 atctgtttgc agctcacccg ccgctacgtc gatccgaccg ggctggaact cgattggcgc
    11701 aaatacgacg agatcaaggc ccgcctcacc tacttcgagc agaaagaaga ctacaaggtc
    11761 atcagcgtca atgagcagtt gacgacgcgc tcctttgaat ccctgggcgg caccacgtcg
    11821 cagggcgaat tcggcagcat gctggccgaa ctgtttgcgc cggaaacggc ggccgagttc
    11881 gagtgggatc accacagcgt actgcgggac cgcaaggtat acgtgtttcg gactcatgtt
    11941 cccaagagcc ggtcgcaatg gcatatcagc ttccagcgcc agagagaggt catagccggc
    12001 taccatgggc tggtgtatat cgacaaggac aacctttccg ttctgcgcct gagcctggtg
    12061 actgacgagt tgccgtccga ttttccaatc accgaagcgc ggacggcgct ggattacggt
    12121 tacgcgaaga ttgccgaccg cgactatctt ttgcctttgc acgccacggt gcgcctgcgc
    12181 gaatcgaaat ttctcacccg caacgaggtg gagttccggc tctatcgcaa gttctcgacc
    12241 gaggccacca tcacgtttga tgatgaagct aagggagtcc agccggaaga aaccaagcct
    12301 cccgccgaac cgcaaaattg aacccgccat ccagccaagg tctcgaacca ggcccgtccc
    12361 tctgaggccg cgggaggaaa ggccaagccg ccacacagtt ccgttggtca gggcttgcgc
    12421 ccaccctcga gcaccgtgcc gtgctgccac ccaaaatcgt gatacttgtt aaaccatgag
    12481 agttgcctgt cccatcctgg cgggtttcgc ggccgccggg gttcttttgg cagttaccgc
    12541 cggatgccag cagaagcgcg caagacaagc cgctcgcccc ctgatcgatc ccgaccgggc
    12601 cattgacctc acctatccgc tcgatgactc caatatctat tggcccaacg ccaagccttt
    12661 ccattatcag cgagacgccg gcggcccaac ccccggcggc tattggtgcg ccgcaggcag
    12721 cttctcagcg agcgagcacg gcggcactca tatcgatagc cctgtccact tcgccgaggg
    12781 gaaagcaact gtggatgaaa ttttgctggg ccggctgatt gcgcccgcag tgctgattga
    12841 cattaccgca gcctgcgctc aaaaccggga ttacgagctg cgcattgagg acattaccga
    12901 atgggagcgc atccacggcc ggattccgga cggcgcagcc gtcctggtcc gaacgggatg
    12961 gagtaaatac tggccggaca agaaacaata tctcggcagc gatgcgccgg gggacgtctc
    13021 ccacctgcac tttcccggca tctcgcagaa tgcggcggag tttctggccg ggatgcggaa
    13081 gatctatggc gtggggatcg acaccgccag tctggatccg gggacgtcga agaccttcat
    13141 ggcgcaccgc atcctcgccg gcgcgcgcat ctatggactg gaaaatgtcg ccaatctgga
    13201 caaggtaccc gccgcaggcg ctacgctgat cgcgctgcct atgaaaatcc gcggcggcac
    13261 cggcggcccg acccgcatca tcgccatatt gccgtgagcg gagtctgacg aagcgccgat
    13321 ttcatcgcaa gagcaaagac cttcagtgcc gggttgtttg cgacacgcgg tcagggaagc
    13381 aggcgctagc ggcgggcgca ggttgccgtc aatacgtcca gaatgcgctc gctggcccgg
    13441 ccatcccagc gctcgggaac ccggctccgg cctcggggcg attcgagtgc ggcgattgcg
    13501 gcttcgacaa tatgagcggg atcgccgccc accaggatgt tggtcccctc ggcgatggtc
    13561 accgggcgct cggtgttggt ccgaagggtc aggcagggca cctgcaagat cgtggtttct
    13621 tcctggatgc cgcccgaatc ggtcaacacc aggcaggcat ggctcaccaa gtggagaaaa
    13681 tcaagataac ccagcggaga gatgaggacg actccatcag agccgggctg caaggttttc
    13741 agcatttgtt ccgttcgggg atgcacagga aaaatcacgg gcaaacgccg ggcaatctcg
    13801 gtgagagcct tcatcaagcc ggcgagcgca tcggcccggt ccacattcga cggccggtgc
    13861 agggttgcca cggcgtattc cccggcgcga agacccaggc gcgccagcac atccgacatt
    13921 tcggcttttt tctggaaacg cagcagcgta tcgatcatca cgttgccgga gaaaaatatc
    13981 ttttcgcggg ggacgccctc ggccaggaga tttttctccc cgctcggctc gctcacaaaa
    14041 agaaagtcgg atatggcgtc ggtgagtacg cggttgattt cttccggcat cgatcgatca
    14101 aagctccgca ggcccgcctc cacatgggct accggaatcc ccagtttgac gccggccagc
    14161 gctccggcta aggtcgagtt tacgtctcct accaccagaa tcacatccgg ccgcgactgg
    14221 ttcaggaccg gttcgaggcg tttcatgatc tcggctgttt gccagaggga agtacccgat
    14281 ccgacgtcga ggttgacatc ggggggcggt atgttgagat cggagaaaaa gctcgccgac
    14341 atctccgcgc tgtagtgctg gccggtgtgg atcaactggt attgaaggcc gggtctgcgc
    14401 cgaatttcgg cgaccagcgc cgccatcttc acaaaattcg gccgagcgcc ggcgatgcag
    14461 gcaagcttca tcgtttttcc gcaactacct gaaagaaagg tccgaaacaa tagaatacgc
    14521 cgcgcagtag ggggacgcgg cgcagcgcca gatacgcttc ggccaggcgg cgcttgagcg
    14581 gtggaaaatg ctgtagctgg cgcggactga gcgctggaat gagcaatcgg gcccttccgg
    14641 gaaagtgcgc gtgaagcatc cgggagagcg accgggctga aagcagccgt acatcgtcat
    14701 agcaaatgcg ccgccggcgc agcacatagc ggttcgccaa gcgccgcggc agatacccca
    14761 cgccccacaa gccgacgtgc ggctccggcg agaggctgaa gcgattggga gtggaaagaa
    14821 acaagggccc cccggccacc aacaccctgc ccatctccgc caaagattgc tggggatcgg
    14881 cgaggtgctc gataacatcc gccgcgatca cagccgcgaa ggcgccgtcg cgaaaaggca
    14941 gccgcccggc atccgccgcc gccagcagcg cttgcggccc cagccgcttg cgcgtgagca
    15001 cgagaaacgg caggcacaaa tccacgccag ccacatggtc ggcgcgctcg gcggccacag
    15061 cgaggtattg acccacgccg cagcccacct cgagaacggc ttcgccgggc aaaaactccc
    15121 ggctttcctc aatcagttcg agggccgcgc gcgcttgatc ctcctcgcca gccaggtgcg
    15181 ccatgtgctt gtcgtgcaag tcctcgcccg gctcgggatt caaccgatag tagaggtgca
    15241 gcaggccttc gaaatcgagg tccttttcgg attctaccag caccctcgcc gtttccgtgt
    15301 cgcgcgacag atcgaaatgc gccggcaggg aaatgcgaaa atcagggatg ccgaagacaa
    15361 agggatagag ccgcgcgcat tcggcgcact cgtatccctc gctggcgacc ctcaccggac
    15421 cccggcatat cgggcaacag aaatcgatcg gcaatcggct gctattataa ctttttcgcc
    15481 ttttatccca tgctgcgcac agcgatactc ggactcggcg gcgtggccga gcgcattcat
    15541 atcccggcct gtctcgcggc tccgggaatc gagctggccg gagcggccga gccggtggag
    15601 gaagtgcggc ggcgcatggc gcagaaattt cgccttccag ccgtttatga gagcgcggaa
    15661 acgctgttgg agaaagagaa gccggatctg gtcattatcg gcgcgccgcc ggactcgcac
    15721 cgcgatcttt gcctgctaag cttttcgcac ggcgcgcacg tcttttgcga aaagcccttc
    15781 atgcgcacgc tcgcggaagc cgatgaagtc atcgccgccg cccgctcgca cggacggctt
    15841 ctcgccgtaa acaaccagta ccgctatatg ccgatttaca gccggacgcg cgagcggctc
    15901 gaccgcggcg attttggccg cctctatctc attcagtgct ggcagcagat gttccatccg
    15961 ccggcggtag agaaagtggc atggcgggcg gcgctcaagc aatccactct ttatgaattc
    16021 ggtacgcacg cgctcgattt gatgtgttac ctgttcggct cgcttccggt cgctctcacc
    16081 gcccagattc ccgctgcgca cccggcttac accagcgatg tggtagtcct gatgacgctg
    16141 cggttccccg cagagcgagc cgccacgctg gtgatgaacc ggatcagccg cgcgccggag
    16201 cgctacctcg agatgcggct ggattgcgag cgcgcttcgc tccgcctctc gctcgggggc
    16261 gtcgcccggg cgtcggtgga gtggtcgaag cgcctcggcc gtcccaccgg gcgcttcagt
    16321 ttcgtgaaag gcggcgaagc ccgcgaagaa tccggggggc ggtcgagcgc gtacgttcag
    16381 gaaaagagtc ccgccttcat gccggccacc gccgcgcatc tccggcggtt cgccgccgaa
    16441 atcgaaagcg gctcccgaaa ttacgagccg gcgctgcacg cccgggaggt gctggccctc
    16501 gctctggcag gctatgaagc cgcagcttcc ggcgagacaa tatggtttca gcgctaacat
    16561 atcagcagat cttgggagat cccgtatgag tgcgttcgtc aaagtggcaa aggccagcga
    16621 tattccggcc ggaagcggca aggtcgtcga agccggcggc aaacagatcg ctatcttcga
    16681 ggcgggcgga aaatactacg cgatcgacaa cacttgcacg catcgcggcg cgccgctggc
    16741 cgagggagta attcagggaa cggaaataga atgtccgtgg catcgagcac atttcaatct
    16801 tgaaaccggc caagctacgc gcccgccggc ggcgcgcagc gtcggcgtgt acccggtccg
    16861 ccagcagggc gatgatattg aaatcgagat ctgactggcg ccgggttgcg tagcgcgccg
    16921 gcgaatgccg ccagcggcgt cggcgcgatt ttctcgcgga ggtcctttcc ccagacatgc
    16981 ctcctcgcat tctcgacgga atcagccaac tgcgatcgct ggccggccag gaagccggcg
    17041 ccagcggctg gcttgaggtg acgcaatcgc tcgttgacga gtttgccgcg gcgacaggcg
    17101 accgccagtg gatccacgtc gatcccgccc gcgctcaagc cgaatccccc ttcggccgca
    17161 cgatcgccca cggtttcctg acgctctccc tgttaagccg gctggcatcc caagccatcg
    17221 aggttcgcgg ctttcggatg tcgatcaact acggtctgaa tcgcgtgcgc tttcccgctc
    17281 ctgttccggc aggatcgcgc atccgggcgc attttacatt gcaatcgatc gaagagcttg
    17341 cgcgctgcgc ccaggcaacg tgggcggtga tcgtagaagt ggaaggagcg gcaaagccct
    17401 cgctggtggc cgagtgggtg gtgcggtatt atcaataagc ggcttcgcgg gagcaaccat
    17461 cggtccggaa gagcaaaggg aataaatcgc cggaatcccc agtcataatc cttggaaaag
    17521 caggaaaaac tcgcgcgttt cgagcagact gtcatgccgc acctgcgcgc agcctacaat
    17581 ctggcgaggt ggctggctcg cgacgcccaa gacgccgaag atatcgtcca agaggcgttt
    17641 gtgcgcgcat tcggcttttt tgagggcttt cacggtcagg acgcccgcgc ctggctgctc
    17701 gcgatcgtgc gaaacaccgg ttacacatgg ctgcggcaga atcggtcccg gcaattggag
    17761 acagctttcg acgaagaggt gcacagcccg gaagccggct cgttgaatgc ggaggcttct
    17821 ctgctccgag aggcggattc acgccttgtc caggacgcgt tagaggccct gccgcgggaa
    17881 tcgcgagagg tcctcgtgct gcgcgagttg gaaggtcttt cctacaagca aatctctgaa
    17941 gtcgcgggcg tgccgctggg gaccgtaatg tcgcgcctgg cccgcgctcg ccaggcgctc
    18001 caggcgcggc taggccgaaa gccctcaggc cagggccgcg caaaaaagga agccgatgaa
    18061 ttgcagcgag attgaagagc tgattcacgc ttacagcgac ggcgagttgg atttaatccg
    18121 cagcctcgag atcgaacagc atcttcggac gtgcgccgcc tgctcccgtt cgcaccagga
    18181 actcgaagcg ctgcgctcgg caatgcgcgc tgcacctctc tatttcgagc tgcccaagca
    18241 gctccagggg caaattcact cggccttgga gaaaaccggc ggcgagcaga ctatgaggac
    18301 gccggcgaaa cgtcgtgctg cctggctgcc gtggctgaga ggcgcggccg ccgccgctct
    18361 tatcgctgtt atttttggtg cgcttgcgcc gcttttctcg cgccggccgg ccgacgatct
    18421 tctggcgcag gaagtcgtgg ccagccatgt ccgttcactg atgcccggcc atctgacgga
    18481 tgtcccctcg accgatgagc acacggtcaa gccgtggttc aacggcaagc tggatttttc
    18541 tcccactgtg aaggatcttg ccgcccaagg gtttaccttg gccggcggcc ggctcgatta
    18601 cctgcaaggc aggccggtcg ccgccctggt ctaccagcgc cgcaggcact acgtgaatgt
    18661 gtttgtctgg ccttcggcgc gcagcacggg cggcgaagag aggccatctt cacgccaggg
    18721 ctataacctc gtgcactgga cccaatccgg catgacgtac tgggccgtgt cggatctgaa
    18781 cagcggcgag cttcaggaat tcagccgtct cctgcaaacc ggagggtccc tgcgctgagc
    18841 ggcgctggcc gcctcttact aagatcttgc ctggcttccc tttgatcatt tgtagccgcc
    18901 gcagctttta ccgctgttaa ctatgcgccg cgactgtttg ctggcggaat attcggaccg
    18961 tgcgcgcagt cttgcatgac gaagcaatca aattccatcg aggagacaga catggcaaaa
    19021 cgaattgttt acgatcacaa tcccgacggc attgaccgcc ggggctttct ggaatgcatg
    19081 gcctgggccg gaaccgggat tctctggacc atcacagccg gagttccgaa gtcctatgcc
    19141 ctcccacagg cggatcacaa gacgagccac agaaaggacg gcctgagctt tatgcagatc
    19201 agcgacagcc atatcggatt cagcaaggaa gcgaacaagg acgtcataac tactttgcaa
    19261 gccgcagttt caaagatcaa cgccgctccc gagccgccgg catttcttat tcataccggc
    19321 gacatcaccc atctctcgaa gcccgaagag tttgacatcg ccgaagaagt cttgaaaggc
    19381 gcaaaagcga accgtgtgtt ttacgtgccc ggcgagcacg acgtgctcaa cgataacggc
    19441 aagcaatatc tagaacgtta cgggaaagag acgcgcggcg gcggctggta cagtttcacc
    19501 aagcagggcg tgcattttat cggactcgtc aatgtcctcg acctcaaagc cggcggcctg
    19561 ggccagcttg gcggcgacca gctcgagtgg ctgaagagag acctggccgg gctctcgaac
    19621 agcacgccgg tcgtggtctt cgcgcacatt ccgctctgga ccgtctaccc ggattgggga
    19681 tggggaaccg ccgacagcga gcaagcgctc gcgtacctca agcgctttgg ctcggtgacc
    19741 atcctgaacg gccacataca ccaaacgttg caaaaggtgg aaggcaacgt cgttttccac
    19801 accgccatgt ccacggcgtt cccgcaaccg gcccccggcg cggcgccttc acccgggccg
    19861 atgaaagtgc ccgccgagaa gctccgcagc gtgctcggcg tgacgagcgt gaagtacgtg
    19921 cctggcagga gatccctggc catcatggat tcgccgctcg aggcggcgcc cgtatcaggg
    19981 cccgctgcgg cgcaaatagc gattgataac ttcagcttca ttcctgctga gatcaaagtc
    20041 ccggcgggcg gcacggtcgc ctggacgaac catgatgatg tgccgcacaa cgtcatcagc
    20101 acggaaaagc ggttcgcgtc gccggtgctc gatacagatg aaaaattttc ttacacattt
    20161 cagaacccag gcgtttaccc gtattactgc tcgatccatc ccaaaatgac cgccaaggtc
    20221 attgtcgagt gagattcggc gtaagcggga gatagctcgg gcgtttggct gcggtcccgg
    20281 catcatccag ccacggtcac gcccagaatc ttgccgattc cgaaggtcag cgccgcggcg
    20341 ccgaggccga taattaactg acgtacaccg gagtacagaa tcgttcgtcc cgtgagcagg
    20401 gtgatcgcgg cgccgctgcc aaacagcccg agggagctca tgaccaggct ccccaggacg
    20461 gcgggatagc cgctaaggaa catgaagggc gctaccggaa gaattgctcc ggtcacaaac
    20521 agcacgaagg acatcacagc ggcttcccac gccgagccgc ccagagtttc cgggtcgatg
    20581 cccaattcct cgcgcgcgag cgtatcgaga gcggtggctt tatcgctaac gaggcgattg
    20641 gcgacgctgc gagcctcccc ttcgctgagg cccttcgact ggtagatcaa agccagctct
    20701 tcggcctctt cgtcgggaac ggcgttgagc tcttcggcct caacgccgat ctgccgctca
    20761 taaagctcgc gcgaactggt aaccgagagc cactcgccca aggccatgga gcacgcgccc
    20821 gcgagcagtc ctgccaggcc tgtgatcaga atggcccggt ttgacaggtt ggccccggcc
    20881 acgcccatta ccaaactgag gttggatacc agcccatcgt ttacgccgag cacggatgcc
    20941 cgcagagcgt tgcccccgat ggcgcggtga cggccttcca gttgcgcgag cacgccgcct
    21001 tcgattcccc gagggccggc gatcgtgcgc agcagccgcg cgtgggactc ttcctcggcc
    21061 ggcataccgg acggcttggt ttcaggttgc agcaaatagt cgtggcggtc ggcttgctcc
    21121 atggcgctga tggttggcag cacaaactga ggtccgaacc gccctgccag ccagccaagc
    21181 agccgcgagc gccaccccgg cttgcgccgc ttcatgacca cgccggcctc ccgcagcttc
    21241 tgttcccaga agtgcgcgtg ccgctcctcg acggccgcca gacgccggta gacctcagcc
    21301 aactgcggct ggcgctcaca gcgggccagc gtgttgtaca gcgaaacgct gtcaatctca
    21361 tcctggagat tcgcccgata gcgcgcgatt tcttgtttgg tgctcatggg tcagcctcca
    21421 aacagccggt tggcctcggc ataggggatg gcgttcccgg taaagctggc aaacgtcccc
    21481 cgctccttca attctccggc aatttgccgg atgagggcca gggtcgcgcg cataggtcct
    21541 gaaccggaac tcacccgcgc cacgcctagc cgctccagtt ccggagccgg cggaacaccg
    21601 ggaccggcca aaatgttgat aggcccacga atctcttgcg ccagacggcg gattagggtg
    21661 gcatcgcgca cgccggggac aaacaagcaa tccgccccgg cttcccgata cgcgttggcg
    21721 cggcgcacgg catcctcgaa gcggccctct ggcgccccta c
//
LOCUS       DASREK010000234        12345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2257466, whole genome shotgun sequence.
ACCESSION   DASREK010000234 DASREK010000000
VERSION     DASREK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12345
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257466"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFA54_12280"
     CDS             <1..276
                     /locus_tag="VFA54_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZS51635.1"
                     /translation="FGGGQVDITSGKFVFSASESYWIEDGKITRPVRNATLIGNGPDV
                     MTKVTRVGHDLKLDQGIGTCGKEGQSVPVGVGIPTIRIDRLTVGGVG"
     gene            370..1707
                     /locus_tag="VFA54_12285"
     CDS             370..1707
                     /locus_tag="VFA54_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HZS51636.1"
                     /translation="MIELANDVVSRAIRRGAADAECTVVEGEEFSASVRLGEVETVKE
                     AGSKRIGLRVLVGRRSGSSYTSDFSRSGIETLVDSAIALARITSEDPCAGLPDAAELG
                     ASGCDSGLYYDDVVGLAALEKIDLARRAERAALETDSRIGNSEGGGFESSWSRRAFAN
                     SRGFQGEYRRSSCSLYAVPVARDAGQMERDYWYSLSRSLARLDPPEMVGRIAAERALR
                     RLGAVKIETKRVPVVFEPRTARTLLDNLFEAVSGDSIYRQASFLAGKLGETVAAPGVT
                     VVDDGTMPGGFGTSPFDDEGAPTRRTVVIRGGVLENYLLNTYTARKLGMKTTGNAARS
                     LAGNPGIGRGNFYLEPGVYRPEEIIASIDEGFLVTELIGFGVNLVTGDYSRGASGLWI
                     RKGKPAFPVHEVTVAGNLGQMLQDIEMAGSDLEFRGPVAAPTIKIREMTVGGR"
     gene            1710..2171
                     /locus_tag="VFA54_12290"
     CDS             1710..2171
                     /locus_tag="VFA54_12290"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HZS51637.1"
                     /translation="MARREAPPVPVLVAGALLLICGTIAFFWYRQAQNRPREVPVLTR
                     EAAQYLPNLQLEDVQMSAAENYLNQTATTITGKITNTGPRTLRLVEVNCVFKDAAGQP
                     VLRERVAIVGRKTGPVPSGQTRSFELSFDNIPASWNQAMPDLVISQILFQD"
     gene            complement(2201..2938)
                     /locus_tag="VFA54_12295"
     CDS             complement(2201..2938)
                     /locus_tag="VFA54_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HZS51638.1"
                     /translation="MRYLILSDIHSNWEALEAVLSAAEGLYEKIVCLGDLVGYGADPN
                     RVVDWVRANVPVVIRGNHDKACSGLENLEWFNEVARQAALWTLSRLTDENRQYLVSLA
                     RGPLVTNGMTLVHGSILDEDEYVVSPREAAEVFPYLERPLTFFGHTHIQGGFVQSRER
                     LQLIRPQPDKGPQYLEIQADETYLLNPGSVGQPRDGDWRSGFLIYDSEDRLIVFYRVE
                     YDLATAQEKIRIAGLPEHLAARLEVGR"
     gene            complement(2944..4143)
                     /locus_tag="VFA54_12300"
     CDS             complement(2944..4143)
                     /locus_tag="VFA54_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZS51639.1"
                     /translation="MQTVHISYWFDGVVGATVVACQFQIYRWIRRALVKHLAPGQAKL
                     ACAALLVFLAAPLLVIELNSESYFWQPLPFTGRAAAFAAGIWVAGSTGAVLLLAAGAF
                     LRRVPARAASSPRSRGGFVAGRRRLLETAVQAAAALAPFGVAGYGTLIVRKNFLLREA
                     DFPVSGLHPDLEGLRMALLSDIHLSPYLSLPELDRVIAMANETRPHIALVTGDLITRQ
                     GDPLETCLARLARLRADAGVFGCLGNHEVYADCQDLAATWGRALGIEFLRLEHRVLRF
                     GNAHLNIAGVDYQRQRRPYLVGAEWLLEPGTVNLLLSHNPDVFPVASRLGYDLVVGGH
                     THGGQVTIEILEQTLNASRFFTPYVAGLYRRPGSALYVSRGIGTINLPMRLGATPEVA
                     LLRLRRA"
     gene            complement(4243..5043)
                     /locus_tag="VFA54_12305"
     CDS             complement(4243..5043)
                     /locus_tag="VFA54_12305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HZS51640.1"
                     /translation="MSDHRLGFVRAGFAAGRQLSCTEGVCLARRRFFVERTDPGRAVV
                     RGEQAHHLHNVLRLQPGQIFELSDTRQLYLGRVIASLPGEVTFAIEKELPAGPQPPEV
                     TLLGAIFKFDRYEWMIEKATELGAARIIPVITSRTDPKLAAAAQKRVERWRRIAFEAA
                     QQSRRVAPPELPQPVALDSALAAAPEGRRWILEEGLEPASLSRLGPARDRASVLLIGP
                     EGGWTEGERKMAHQCGFLPASLGPLILRAETAAIAALALLLLPAASDF"
     gene            5068..5144
                     /locus_tag="VFA54_12310"
     tRNA            5068..5144
                     /locus_tag="VFA54_12310"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5102..5104,aa:His,seq:gtg)
     gene            5257..7983
                     /locus_tag="VFA54_12315"
     CDS             5257..7983
                     /locus_tag="VFA54_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS51641.1"
                     /translation="MALQAQSTAVTGTIQGIVLDSSGAPMPNVQVTAVHVDSGAVRQT
                     STGDSGQFRLTGLAIGNYELRAEREGFGTVVTRLQVSVGQTVVQRVEMAPAAVSQSME
                     VKEQSEALDVAATTSGATLGSERIEEAPAQNRNYLNFVLVAPGAAPSSGSNPQRSSAG
                     VRSAAPDSGFSFGGMRPRNNSLSIDGVDNRDETTGANRVAIGLEMVEEFRVAGTAVGA
                     ELGGASGGIVNVVTRSGTNIWHGDWTFFGQNEAANARNPEAPAPNKPRFRRYQPGSSI
                     NGPIRRDQTFFSTAFEQEWESSEEWSEGPAAAQPAINAVLARPELANAAVDSIRRGLF
                     RASSAQSEFSVKLNHQLHSIHALSVRYALSHGRVSNDVQGTENFSDRSSRGSSLTTDH
                     SLVAGLISVLSPRLVNDLRFQAARRDVDLTPNSRGAMMEIPGVVAFGQAYGLNGRRSE
                     DHNEMVEGLNLAAGQHHLSFGASAHLVRLDARLADRFAGIFVFPTLADFLAERPDVFV
                     QAFGNPATEARVAPLGAWAQDRWQPLAGLSLEAGLRYDYEILPRGFSSPSRNFAPRFG
                     IAYRPGGKGPLVFRAGFGLFYDRYPLAYLNNVLQKDGIHGFEQYLVGDMAVAALAAGR
                     GGSLSSPMPNTAPGIYKPSPDFSATYSRKFVGGVERSLGQNTSLTAEYASVAGFHLPR
                     IRNIAATLPPLYQLEQTARSSYQGVSVSINRRMSRELTFLAAYNLGRTWDDSSDYDEQ
                     PLDPRNLRQDWARSRQHQAHRISASAVFELPAEEAAFLPKWVANELENVVMAPIFTAG
                     SGRPVNALDSTDVFRTGAYPISARPFGLPRNPFFGPGTVSLDMRIMKGVWLKKNRMIL
                     QTGVEAFNLANHTNALRVSPYYAAAGQILGSYRGITEALNARQLQFVFQIEY"
     gene            8041..9717
                     /gene="ilvD"
                     /locus_tag="VFA54_12320"
     CDS             8041..9717
                     /gene="ilvD"
                     /locus_tag="VFA54_12320"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZS51642.1"
                     /translation="MKLQSYTITQGRDRAPARAMLKAIGFTDEDLRKPIIGIANTWIE
                     TMPCNYNLRELAVKVKEGVRAAGGTPMEFNTIAISDGVTMGTEGMKASLVSREAIADT
                     IELVARGHMFDGIIALVACDKTIPGGAMALLRLNVPGLVLYGGTILPGYHNGKALTIQ
                     DVFEAVGANAAGRISDAELLAIENRACPGAGACGGQYTANTMATVMELIGLSPMGTAS
                     VPQVDPRKNDVAFRCGQIILEAVKKDLRPRDIATRKAFENAIAGVAATGGSTNAVLHL
                     LAMAREAEVPLTIDDFQTISSRTPLLADLKPAGRFVAVDVDKAGGIPVIAKRLVEGGF
                     VDGSAITITGRTFAEEAAEAKETPGQEVIRPLGNPIKPTGGLVILKGSLAPEGCVIKT
                     TGMTRKQHRGPARVFNREEDAMGAVTEGKIKAGDVLIIRYEGPRGGPGMREMLGVTGA
                     IVGAGLSESVALVTDGRFSGATHGFMVGHVAPEAASGGPIAAVQEGDLISLDIEARTI
                     DLEVPPETVKQRLAAVKPPLPRYKTGVFAKYVALVSSAAEGAITSQVGNR"
     gene            complement(9786..10553)
                     /locus_tag="VFA54_12325"
     CDS             complement(9786..10553)
                     /locus_tag="VFA54_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS51643.1"
                     /translation="MFTPFRLDNRHALVTGGASGIGEATVRVLAAAGARVTIADVNRE
                     RAEALASELGPAVSAAIVDVTDEHSVRNLFAAIPALDTLVNNAGTGLVGSVEETDFAD
                     WQRLFRVNVDSIFLCTKYAMPLLLASGSRGFPGTIVNIGSVAGLGGVKRRFAYCATKG
                     AVVALTKQLAGDYPGRLRVNCICPGTVESPFVEAYLEKYHKHEKEKVRAELHQRQPVG
                     RMGRPVEIAYMVLYACSDEAEFVTGSTLVIDGGWTAI"
     gene            11169..12140
                     /locus_tag="VFA54_12330"
     CDS             11169..12140
                     /locus_tag="VFA54_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZS51644.1"
                     /translation="MEYRILAGTDLKVSRVCMGTMTFGSQVDEAGSRNMVDRCLDAGV
                     NFFDTANSYNKGLSEEIVGNVLAGRRNRVVLASKVFNKMGDGPDDSGLSRGAIHKQIE
                     ASLKRLKTDYLDIYYLHQPDYKTPIEETLSAMEDLVKQGKVRYPGTSNYAAWQVCQAL
                     WICQKNGWRPPVISQPMYNLLARGIEQEYLPFTREFCVSSIVYNPLAGGLLTGKQRRE
                     TGPIAGSRFDKNQMYLNRYWHEANFQAVDELARIAAGSGRSLVQLSLTWVLQQPGVDG
                     VLIGASRMEQLEENLKAVEGKPLDQPVLEACDRVWANLRGVTPKYNR"
BASE COUNT         2471 a         3768 c         3838 g         2268 t
ORIGIN      
        1 ttcggcggcg gccaggtgga catcaccagc ggcaaatttg ttttttcggc ttcggaaagc
       61 tattggatcg aggacggcaa aatcacgcgc cctgtccgca acgccacact gattggcaac
      121 ggcccggacg tgatgaccaa agtcaccagg gtggggcacg atctcaagct cgaccagggc
      181 atcggcactt gcggcaaaga ggggcagagc gttcccgtag gcgtaggaat accaaccatc
      241 cggatcgacc ggctcactgt gggcggggtc ggatgagaaa acgagtacga aagtcgagct
      301 ggccggcggc cgtttccccc ggcccccttt cggatctggc gatttcggcg cagaagcgat
      361 catccttcaa tgattgaatt ggccaacgat gtggtctcac gcgctattcg ccgcggcgcc
      421 gcggacgcgg aatgcacggt tgtcgagggg gaagagtttt ccgccagcgt gcgtttgggc
      481 gaggtagaga cggtaaagga agccggatcg aagcgtatcg gcctccgcgt tctggttggg
      541 cggcgcagcg gctcctccta tacgtccgat ttctcccgta gcggcatcga aacgctggtg
      601 gactcggcga ttgctctggc tcggatcacg tcggaagacc cttgcgccgg gttgccggac
      661 gctgcggaac tgggagcgag cggctgcgat tcgggtttgt attacgacga cgttgtaggc
      721 ctcgctgccc tggagaaaat tgatttggcg cggcgcgccg agcgggcggc gctcgagact
      781 gactcccgca tcgggaactc cgaagggggc ggcttcgaaa gctcttggag ccggcgcgcc
      841 ttcgccaact cgcggggttt tcagggcgaa taccggcgca gttcctgctc tctttacgcc
      901 gtgcctgtgg cgcgggacgc gggtcaaatg gagcgcgact attggtacag cctatcacgc
      961 agcctggccc ggctggaccc gcccgaaatg gtcggacgca ttgccgccga gcgggccctg
     1021 cgccgcttgg gcgctgtcaa gatcgagacc aagcgggttc ccgtcgtgtt tgagccgcga
     1081 acagccagaa ccctgctgga taacctgttc gaggcggtta gcggcgattc catttaccgc
     1141 caggcttcct ttttagccgg caaactgggc gagaccgtgg ccgctcccgg cgtgaccgtg
     1201 gtggatgatg gaactatgcc cggcggtttt gggacgtccc cgtttgacga cgagggagcg
     1261 ccaacgcgcc ggaccgttgt gattcgcggc ggcgtgctcg aaaattactt gctgaatacg
     1321 tacaccgccc gcaagctggg catgaaaacc accgggaatg ccgcgcgcag ccttgccgga
     1381 aatcccggca tcggccgggg gaatttttac ttggaaccgg gcgtttatcg ccccgaggaa
     1441 attattgcct ccatcgacga aggatttctt gtgaccgaat tgatcggctt cggtgtcaat
     1501 ctggttaccg gagactattc gcgcggcgcg agcggcctgt ggattcggaa gggcaagccg
     1561 gccttcccgg tgcatgaagt gaccgtcgcg ggcaatctcg gccaaatgct gcaagatatc
     1621 gaaatggccg gctcggattt ggaatttcgc ggcccggtgg ccgcgccgac gattaaaatt
     1681 cgagagatga cggtaggtgg aagatagctg tggcgagacg cgaggctccg cccgttcccg
     1741 tcttggtcgc cggcgctttg ctgctgatat gcggcacgat cgcctttttc tggtaccgcc
     1801 aagcccagaa tcgcccgcgg gaggtcccgg tcttgacccg tgaagccgcg caatacctcc
     1861 ccaatctcca attggaagac gtccagatga gcgctgctga gaattacctg aaccagaccg
     1921 ccaccacgat cacgggcaaa atcaccaata ctgggccgcg cacgcttcgg ctggtcgagg
     1981 tcaactgcgt tttcaaggac gccgccggtc aaccggttct tcgtgagcgg gtggccattg
     2041 tgggccgcaa gaccggcccg gttcccagtg gccaaacacg ctcgtttgag ttgagcttcg
     2101 ataacattcc ggcaagctgg aaccaggcaa tgccggatct ggtgatttcc caaattcttt
     2161 ttcaggactg aaacgcagtc cagacgcggt tccggggctt ctaccgtccc acttcgagcc
     2221 tcgcggccag gtgctcgggc aggccggcga tacggatttt ttcctgcgcg gtagccaggt
     2281 cgtactcgac gcgatagaac acgatcaggc gatcttcact atcgtaaatc aagaagccgc
     2341 tgcgccagtc tccatcgcgc ggctggccga ccgagccggg gttcaacagg tacgtctcgt
     2401 cggcctgaat ttccagatat tgcggtccct tatcgggctg cggccggatg agctggagcc
     2461 gctcccggct ctgcacgaac ccgccctgga tgtgggtgtg gccgaaaaat gtcagtggcc
     2521 tctccaggta aggaaaaact tcggcagcct cgcgcggtga aaccacgtat tcatcttcat
     2581 ccaggatcga gccgtggacg agggtcatgc cgttggtcac cagggggcct ctggccagcg
     2641 acaccagata ctggcgattc tcatcggtaa ggcggctcag cgtccacagg gcggcctggc
     2701 gcgcgacctc gttgaaccat tccaggttct cgaggcccga acacgccttg tcgtgattgc
     2761 cgcggatgac gacgggcacg ttggcgcgca cccagtccac cacacggttg ggatcagcgc
     2821 catagcctac tagatcgccc aggcacacga ttttctcgta gagcccttcg gcagccgaga
     2881 gcaccgcctc caaagcctcc caattggagt ggatatcgct cagaatcagg tagcgcactc
     2941 tgctcaggcg cgccgcagcc gcaacagcgc gacctccggc gtagccccta atcgcatcgg
     3001 caggttgatc gtccctatgc cgcggctcac gtagagagct gagccgggcc tgcgatacaa
     3061 accggcgaca tatggcgtga aaaaccggct tgcgttcaat gtttgctcca ggatctcgat
     3121 cgtcacttga cccccatgag tgtgcccgcc gactaccaga tcatatccca aacgggatgc
     3181 aacagggaaa acatcagggt tgtgggagag cagaagattc acagttccgg gctcgaggag
     3241 ccactcggct cctaccagat agggccggcg ctgccgctga tagtccacgc ccgcaatatt
     3301 gagatgcgcg ttgccaaatc gcaaaacccg gtgttccagg cgcaaaaact caatccccaa
     3361 tgcccgtccc caagttgcgg ccaagtcctg gcaatcggcg tagacctcgt ggttgcccaa
     3421 acacccgaat acgcccgcat ccgcgcgcag ccgggccagc cgcgccaggc acgtctccaa
     3481 cggatctccc tggcgagtga tgagatcccc ggttacgagc gcaatgtgag gccgcgtttc
     3541 gttggccatg gcgataacac ggtccagctc gggcaggctc aaataagggc tcaggtggat
     3601 atcgctcagc aacgccatcc gcaggccctc cagatcggga tgcagcccgg agaccgggaa
     3661 atccgcttcg cgcagcaaaa agtttttccg gacaatcagc gtgccgtagc ctgcaactcc
     3721 gaacggagca agcgccgctg ccgcctggac ggcagtctcc agcaggcgcc ggcggccggc
     3781 gacaaaaccg ccgcgagacc tcgggcttga agcggccctt gcggggaccc gccgaagaaa
     3841 cgcgccggct gccagcagga ggaccgcgcc tgtcgagccg gctacccaga tgccggcggc
     3901 gaacgcggcc gcacggcccg tgaaaggaag cggctgccag aaatagctct cactattcag
     3961 ctcaatcacc agcagcggag cggccagaaa gaccagaagc gccgcgcacg ccagcttcgc
     4021 ctgccccgga gcgagatgct tgaccagcgc gcgccggatc cagcgataga tttgaaactg
     4081 gcaggccacc acggtagccc ctaccacgcc gtcgaaccaa taagagatat gaaccgtctg
     4141 catagcgacc gatcacccgc cctggagccc ttcgtcttcc attgtaggcg gaatacggta
     4201 aaagaccgga ggctatttcc cgcctcgggc gaatgcctta cgtcagaaat cagacgcggc
     4261 aggaagcagc agaagcgcga gcgcggcgat cgcagcagtt tccgctcgca ggatcagtgg
     4321 gccgagcgaa gcggggagaa atccgcactg gtgtgccatt ttcctttcgc cttccgtcca
     4381 acctccctcg ggtcctatca gcaagacaga ggcgcgatcg cgggcgggcc ccaaccggct
     4441 gaggctcgca ggttcgagac cttcttccag aatccagcgg cggccttcgg gggccgctgc
     4501 gagggccgaa tcgagcgcga caggctgggg cagctcgggc ggcgccacac gccgcgactg
     4561 ctgcgcagcc tcgaaagcga tgcgccgcca gcgttccact cgcttctgcg cggccgccgc
     4621 caatttcggg tccgtacggg aagtgattac tgggatgatt ctggcagcgc ccagttccgt
     4681 cgccttttcg atcatccact cgtaacggtc gaatttgaag attgcgccga gtaaagtgac
     4741 ttcagggggc tggggtccgg ccgggagttc tttttcaatc gcgaatgtca cttctccggg
     4801 gagactggcg atcacgcggc cgaggtaaag ctggcgcgtg tcgctcaact cgaagatctg
     4861 ccccggctgg aggcgcagca cgttgtgcag gtgatgggcc tgctcgccgc gcacgacggc
     4921 gcgccccggg tcggtcctct caacaaagaa gcgccgccgc gccagacata ccccctcggt
     4981 gcaagatagc tgccggccgg cagcgaagcc cgctcgaacg aaaccgagcc ggtggtcaga
     5041 catgttatca tgagcttacc gaatacggtg ggtatagctc agtttggtag agcgtcggat
     5101 tgtggttccg aatgtcgtgg gttcgaatcc cactacccac cccaccgtca agccggcaat
     5161 cttgggtcaa tcgcgaaggc cgcagacgga aaagacggcc gcaagaaatc ctatattatc
     5221 ctggcccaac tgtattttgt acttctcttt tgcggcgtgg cgctgcaagc gcaatccacg
     5281 gctgtaaccg gcacgattca gggcatcgtg cttgattcga gcggcgctcc catgccgaac
     5341 gtgcaggtga ctgccgtgca tgtggattcg ggagccgtgc gacaaacgag caccggcgac
     5401 tccggccagt tccggttgac tggtctggcg atcggcaact atgagctgcg cgccgagcga
     5461 gagggattcg gaactgtcgt cacacggctg caagtttccg taggtcaaac cgtggtgcag
     5521 cgcgtcgaga tggcgccggc ggctgtgagc cagagcatgg aagtcaagga gcaatccgaa
     5581 gcgctggatg tggcggcgac gacatccggc gcaactttgg gcagcgaacg catcgaagag
     5641 gcgcccgctc agaaccgaaa ctacctgaac ttcgtgctgg tggcgcccgg agcggcgccg
     5701 tccagcggct ccaacccgca gcggagctcg gccggcgtac ggagtgcagc cccggatagc
     5761 ggcttcagtt tcggaggcat gcgcccccgc aataacagcc tctcgattga tggcgtggac
     5821 aaccgggacg agaccacggg cgccaatcga gtggcgatag ggctcgagat ggtcgaggag
     5881 tttcgcgtgg cagggactgc cgtgggtgcg gaattgggag gggcgagcgg aggcatcgtc
     5941 aatgtagtga cacggtcagg aaccaatatt tggcacggag actggacgtt tttcggccaa
     6001 aacgaggctg ccaacgcgcg taatcccgag gcccccgctc cgaacaagcc gcggttccgc
     6061 cgctaccaac cgggatcttc tatcaacggc cccatccggc gggatcaaac gtttttctca
     6121 acggctttcg agcaggaatg ggaatcgagc gaggaatggt ccgaggggcc ggccgctgcg
     6181 cagccggcca tcaacgcggt gctcgcaaga ccggaactgg ccaacgccgc cgtcgattcc
     6241 atccgccgcg gcttgttccg cgcatcttcc gcccagagcg agttttccgt caagctcaat
     6301 catcagttgc atagcattca tgcgctgtcc gtacgatatg ccttgtcgca cgggcgggtt
     6361 tccaacgacg tgcagggaac cgagaacttc agcgaccgct cgtcgcgcgg cagtagcctg
     6421 accacggatc attcgctcgt cgccggactg atcagcgtcc tcagcccgcg gctggtaaac
     6481 gatcttcgtt tccaagccgc tcgccgggac gtggacctca cacccaattc ccgcggcgcc
     6541 atgatggaga ttcccggagt agtcgccttt ggacaagctt acgggctcaa tggacggcgg
     6601 tcggaagacc acaacgagat ggtcgaaggg ttgaacctcg ccgcaggcca gcaccacctg
     6661 agcttcgggg cgagcgcgca tctggtgcgc ctggatgcgc gcctcgcgga ccgcttcgcc
     6721 ggaatttttg tgtttccgac gctggcggat tttcttgcgg agcggccgga cgtcttcgtg
     6781 caggccttcg gcaatcccgc aacggaggcc cgcgtggcgc ccctgggagc gtgggcgcaa
     6841 gaccggtggc agcccctcgc gggtttgagc ctggaggctg gattgcgcta tgactacgaa
     6901 attttgcctc gcggcttctc cagcccctcc cgcaatttcg ccccgcgctt cggtatagcg
     6961 tatcgcccgg gcggcaaagg gccgctcgtc ttccgggccg gcttcgggct tttctatgac
     7021 cggtaccccc tggcgtactt gaacaacgtc ctgcaaaagg atgggataca cgggtttgaa
     7081 caatatctgg tgggagatat ggccgtcgcc gccctcgccg ccggccgcgg aggatctctg
     7141 tcgagcccta tgccaaacac ggcgccggga atctacaagc cgtcccctga tttctcggct
     7201 acttacagcc gcaagtttgt cggcggcgtg gaacgcagcc tggggcaaaa tacgagcttg
     7261 accgccgaat atgcctctgt tgcggggttt cacctgcccc gcatccgtaa tatcgcagcc
     7321 acgcttccgc cactctacca actggaacag acggctcgct cctcgtacca gggcgtttcc
     7381 gtgtctatca atcgcagaat gagccgggaa ctgaccttcc tggccgctta caatctcggc
     7441 cgaacctggg acgacagttc cgactatgac gagcagcccc tcgacccgcg gaacctccgg
     7501 caggactggg cccgctcgcg ccagcaccag gcccaccgga tttccgcgag cgccgtattc
     7561 gagcttcccg ccgaagaagc agcgttcctg ccgaaatggg tcgcaaacga gctggagaat
     7621 gtcgttatgg cccctatttt tacggcggga agcggccggc cggtgaatgc ccttgattct
     7681 accgatgtat tccgaaccgg agcctatccg atttccgccc ggccattcgg cttgccgcga
     7741 aatccgttct tcggcccggg cactgtctca ttggatatga ggataatgaa aggcgtgtgg
     7801 ctgaaaaaga accgtatgat cttgcagacc ggcgtcgagg cgttcaacct cgccaatcat
     7861 accaatgccc tgcgcgtcag cccgtactac gccgccgccg gccagattct cgggtcctac
     7921 cgcggcatca ccgaggcgct caacgcgcgg caactgcaat tcgtatttca aattgaatac
     7981 tgagggcgag cggagcgcgc gcttgagccc gcgcacgctt cctactaaga tagaaagtcc
     8041 atgaaattac aaagctatac gattacacaa ggacgcgacc gcgcgcccgc ccgcgcaatg
     8101 ctgaaagcca tcgggttcac ggacgaggac ctccggaagc ccattatagg aatcgctaat
     8161 acctggattg aaaccatgcc gtgcaactac aatctgcggg agctggcggt caaggtcaag
     8221 gaaggcgtcc gggcggccgg cggcacaccg atggagttca acacgatcgc catcagcgac
     8281 ggtgtaacga tgggcacaga agggatgaag gcgtcgctgg tgagccgcga ggcgatcgcg
     8341 gacaccatcg aactggtggc gcgcggccac atgttcgacg gcattatcgc cctggtcgcc
     8401 tgcgacaaga ccattcccgg gggcgccatg gcgttgctcc ggctgaatgt gccgggactt
     8461 gtgctctacg gcgggaccat cctgccgggg tatcacaacg gcaaggcgct gaccattcaa
     8521 gacgtttttg aagccgtcgg ggcgaacgcc gcgggccgta tttcggatgc agaactgctg
     8581 gccatcgaga accgggcgtg cccgggggcc ggggcctgcg gcgggcagta tacggcgaat
     8641 actatggcta cagtgatgga gctgatcggg ctctcgccga tgggcactgc ctcggttccg
     8701 caagtggatc cacggaagaa tgatgtggct ttccgctgcg gccagatcat tttggaagcg
     8761 gtgaagaagg atctcaggcc gcgcgacatc gccacacgca aggcgtttga aaatgcgata
     8821 gcgggcgttg cggccaccgg cggatcgacc aatgccgtcc tgcatttgct ggcgatggct
     8881 cgcgaggccg aagtgccgct gacgatcgac gattttcaga ctatcagcag ccgtacgccg
     8941 cttctggccg acctgaagcc cgcaggccgc ttcgtggcgg tggatgtgga caaggccggc
     9001 gggattccgg tcatcgccaa gcggctggtc gagggcggct ttgtggacgg ctcggcgatt
     9061 accatcactg gccgcacctt cgctgaggaa gcggcggaag ccaaggagac tccgggacag
     9121 gaggtgatcc ggccgctcgg gaatcccata aagccgaccg gcggcctggt catcttgaag
     9181 ggaagtctcg cccctgaagg atgcgttatt aagaccactg gcatgacgcg aaagcagcat
     9241 cgcggaccgg cccgcgtgtt caaccgggaa gaagacgcca tgggcgccgt gaccgagggc
     9301 aagatcaagg ccggcgacgt gctgatcatc cgctatgaag ggccgcgcgg cgggccgggc
     9361 atgcgggaaa tgctgggcgt aaccggcgct atcgtgggcg ccggactttc ggaaagcgtc
     9421 gccctggtca ccgacgggag gttcagcggc gccacgcacg gtttcatggt cgggcatgtg
     9481 gcgccggaag ccgcgagcgg cgggccgatt gccgccgtgc aggagggcga cctgatttca
     9541 ctggacatcg aagcgcgcac gatcgacctc gaagttcccc cggaaacggt caaacagagg
     9601 ctcgccgccg tgaagccgcc gcttccgcgc tataaaaccg gcgtattcgc caagtacgtc
     9661 gcgctggtat cttcggcggc cgaaggagcc atcacgtcgc aggtagggaa ccgttgaagc
     9721 acccagccgg ggcgcaggcg attgtagcgg cctgccagcc cggtctctcc gccgcgcgat
     9781 tttccttata tggccgtcca gccgccatcg atcaccagcg tggagccggt gacgaattcc
     9841 gcctcatcgg aacaggcata gagaaccatg tatgcaattt ccaccgggcg gcccatccgg
     9901 ccgaccggct ggcgctggtg cagttcggcc cgcactttct ccttttcgtg cttgtggtat
     9961 ttctccagat aagcttccac aaaagggctc tccacggttc ctgggcagat acaattcacg
    10021 cgaagccgtc cgggataatc gcccgcaagc tgctttgtca aagcgacgac agcccctttg
    10081 gtagcgcagt aggcgaagcg ccgcttcacg ccgcctaggc cggccacaga accgatgttg
    10141 acaatggttc cgggaaatcc cctgctgccg gaagcgagca acagcggcat ggcgtatttg
    10201 gtgcaaagaa aaatcgagtc cacattgacc cgaaacagtc tctgccaatc ggcgaagtcc
    10261 gtctcctcca cactgcccac caggccggtg cccgcgttgt tgacgagcgt gtccaaggcc
    10321 ggaatggcgg caaacaggtt tcgcaccgaa tgctcgtcgg tgacgtccac gatggcggct
    10381 gaaaccgcgg gccccagttc cgacgccaga gcctcggcgc gctcccgatt gacatccgcg
    10441 atcgtgaccc gcgcgcccgc cgccgccaac acgcggacgg tcgcttcccc gattccgctc
    10501 gcgcctccgg ttaccaaggc atgccgattg tccagacgaa aaggagtgaa cataagagcg
    10561 caaggataac aaaacctcgc gcgtttgagt ttcagcgaac ggagagattt gcgttacggt
    10621 tcagccgggc ttggaaatag acttacaacg acctggcagg gcgccgctgt tatccgagcg
    10681 agatagcaga agcagcgaag cgcgcgtcat acatctaaat aatatctaca agagcgcgat
    10741 accaacgggc ctccgccgca ctctggacga aaagcggccg gcgtgactgc ccggtaatcc
    10801 ggttcttatc gcgaagcggc acaagatcag gacgcggccc gtccgcgctg tctccgccgc
    10861 gccgcccaat acgccttcat acgctcggat tgcgcctttc gagcttcggc cgacatgcca
    10921 cggcgtttgc gggttctcga tgagccgggg gcggcaaaca tccgcctcct ccaccccttg
    10981 cctctgcccg gttcggattg caacgcctca atcgctcgat tcagcttgtc tctttccgca
    11041 atcagcatct gcaagacgtc attggtcata gtagaaacat actaacacac tgagtcaccc
    11101 ggagagccgg accagtttcg gaggcagcgc agtgtgcgag aatacaaagt ttgttctagg
    11161 gaggagaaat ggagtatcgc atactggctg gcacggactt aaaagtttcc cgcgtctgca
    11221 tgggaacaat gaccttcggc tcgcaggtgg acgaagccgg ctcgagaaac atggtggacc
    11281 gctgcctgga cgcgggcgtc aatttcttcg atacggcgaa ttcttacaac aagggccttt
    11341 cggaagaaat tgtggggaat gtgctggcgg gccgccggaa ccgcgtcgtt ctggccagca
    11401 aagtattcaa caagatgggt gacggcccgg atgacagcgg cttgtcgcgc ggagctatcc
    11461 ataagcaaat cgaagccagc ctgaagcggc tcaagaccga ctatctggat atctattacc
    11521 ttcaccagcc tgattacaag acacctatcg aggaaacctt gtccgctatg gaggacttgg
    11581 ttaagcaggg taaagttcga tacccgggca catcaaacta tgcggcctgg caggtctgcc
    11641 aggctctgtg gatttgccag aaaaacgggt ggcgcccgcc agtaatctcg cagcccatgt
    11701 ataacctgct cgcccggggc atcgagcagg agtatctgcc cttcacgcgc gagttttgcg
    11761 tcagctcgat cgtttacaat cccctggccg gtggcttatt gacggggaag cagcggcggg
    11821 agacgggtcc tatcgccgga tcgcgtttcg ataagaacca gatgtatctc aaccgctact
    11881 ggcatgaggc gaactttcag gccgttgacg aattggccag gatcgccgcc ggctccggac
    11941 gctcgctggt gcagttgtct ctcacctggg tattgcagca gcccggtgtg gatggagtct
    12001 taatcggcgc ctcccggatg gaacagctcg aagagaacct gaaggctgta gagggcaagc
    12061 cgctcgatca gcccgtgctg gaagcttgcg accgggtatg ggccaatttg cgcggcgtca
    12121 cgccaaaata taaccggtaa cgcggcagcg cctcctctgg ggaacctggg ttggttatcg
    12181 gacgcgcgat ggcgcatata ttcgccagca ctgctgtcca aattagctga cgacggggat
    12241 tcccgttgtt gaaaacgctg gacatagttc ttcgatcccg gcctttctga aaccaggtta
    12301 ccccccgaga aggaccgagt tgcgcgtgac aaatcactat gctgt
//
LOCUS       DASREK010000235        26756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1591286, whole genome shotgun sequence.
ACCESSION   DASREK010000235 DASREK010000000
VERSION     DASREK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 26756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26756
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591286"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VFA54_12335"
     CDS             complement(<1..825)
                     /locus_tag="VFA54_12335"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HZS51645.1"
                     /translation="MLIVGIGGLTRDAAAAVLRDGTLVAAIEEDKLRGSLWARQPGSL
                     PLEAIRTCLRLADAKPEEVDWVILAGPLGEGASAHLHTQLKSLFPNGRIRVVDHHMAH
                     AASAFYMSPFERATVLTLDRTGDMRCGALWRAEGNTLRLEEELYAPDSPADFYGRITA
                     LLGFRPRSEEHKVQWLSAAGEPKYAGFFRLVMGADSHGWPSFDRSYFDTDHPTHGGFS
                     EKFFAHLGVEGPEGITPEMRLDLAASVQRAVESVVMELAGAGENLCLAGGLALNSLL"
     gene            complement(819..2270)
                     /gene="gltX"
                     /locus_tag="VFA54_12340"
     CDS             complement(819..2270)
                     /gene="gltX"
                     /locus_tag="VFA54_12340"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZS51646.1"
                     /translation="MVRVRFAPSPTGFLHIGGARTYIFNWLFARQKGGAMVLRIDDTD
                     LERNTEASRQSIFEGLRWLGLDWDEEYAQSARAALHRQAAQALLDKGFAYRDFTPAAE
                     AAEAGKDRSGAPWLFNPGQREISREESDRRAAAGERFVLRYRVPRERVSEIRFDDAVY
                     GPQARSTGDIEDFALARSDGTPTYYLASCADDADLRISHIIRGQDHLANTFKHVLIFE
                     GLGAERPELAHLPLLLAPDGSKLSKRRHGQVVSVTAYRDAGFLPHAFVNFLSLLGWSP
                     KDNREVLSRRELIELFSLEGINRNNAVVSYQENDPENWPDPKALWLNAQHLRSMPIEE
                     LLPYVESELRRAGLWQESYAGGQREWFAKTVDLLRARYTTLRDFSRRGAAYFGDQFAI
                     EPGAQEKLQAPGARELLRELGDRLEKIPDFTEENIERELRALAAERGVKAGVIINAAR
                     AALSGQSVGPSAFSVFTAVGRERAIKRLRNGVC"
     gene            complement(2481..2777)
                     /locus_tag="VFA54_12345"
     CDS             complement(2481..2777)
                     /locus_tag="VFA54_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZS51647.1"
                     /translation="MIRVILPPHLRTLAEVEDEVQLDVVGQVTQRAILDALEASYPML
                     SGTIRDHATRQRRPFLRFFACAEDLSHESPDAPLPDEVASGAEPFLIIGAIAGG"
     gene            complement(2774..3889)
                     /locus_tag="VFA54_12350"
     CDS             complement(2774..3889)
                     /locus_tag="VFA54_12350"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZS51648.1"
                     /translation="MSKVRVLVGTRKGAFILTSDGKRERWDISGPHFGGWEIYHIKGS
                     PADPNRLYASQTSGWFGQVIQRSNDGGATWEPVGNKFVYDGVPGTHQWYDGTPHPWEF
                     KRVWHLEPSLSDPDSVYAGVEDAALFRSADGGQTWQEIPGLRGHGSGPRWQPGAGGMC
                     LHTILLDPRDPGRIFIAISAAGAFRTDDGGQTWRPINRGLRSQYIPDPNAEVGHCVHR
                     IAMHPSRPRVLFMQKHWDVLRSDDAGESWREVSGNLPTDFGFVIDVHAHEPETIYVVP
                     IKSDSEHFPPEGKLRVFRSRTGGNEWEALTKGLPQKDCYVNVLRDAMAVDSLESCGLY
                     FGTTGGQVYASADAGDTWAPIVRDLPAVLSVEVQTLP"
     gene            complement(3911..4396)
                     /locus_tag="VFA54_12355"
     CDS             complement(3911..4396)
                     /locus_tag="VFA54_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZS51649.1"
                     /translation="MSLEIFGDRWSLLIIRDLMVRGFRTFKEFQQSGEGIATNILADR
                     LRRLEAAGIIQARRESDARRISYLLTEKGIDLAPVLLELLIWGARHERTAATCAFIER
                     VEKDREKFLAEVRRRWEEGDATPLLPRFGNAGAQHSRAKSGAGARQPARHARATPEGV
                     E"
     gene            complement(4682..6316)
                     /locus_tag="VFA54_12360"
     CDS             complement(4682..6316)
                     /locus_tag="VFA54_12360"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HZS51650.1"
                     /translation="MTRRVFLLAAIAGSLWAQVSFDRILHADREPQNWLTYSGSVRGE
                     RHSLLTQITPENVKNLELQWIYQARSLEKFEATALVVDGVMYTVQAPNDVIALDAATG
                     RIFWTYSYTPSPQARPCCGRINRGLAILGDTLFMGTIDAHLVAIDAKNGQPLWDTTVA
                     RAESGYALAHAPLVVKDKVIVGTAGGEYGIRGFIAAYNARTGKEVWRFYTIPGPGEPG
                     HETWGGDSWQHGGGSIWVTGSYDPELNLTYWGVGNPGPDWNGDPRPGDNLYTDSVVAL
                     DADTGKLKWYYQFSPHDEFDYDSVQVPVLADMEWQGKDGRRTPRKLMFFANRNGIFYA
                     LDRATGEFLMGKPFVTVNWMDGFDSKGKPIRIAGKVPTTEGTLIYPGNQGGTNWYNPS
                     YSPRTGLFYIPTWANYSSIYIKAPEEFKEGQRYGGGFPKSPIPFGRTTQNFRKEDEGY
                     GAVRAIDPRTGERKWEFKMTDVTDAGILTTASDLLFSGGREGYFYALDARTGAVLWKA
                     NLGGAVASGPMTYLAGGRQYVAVAAGNALFSFALRQ"
     gene            complement(6313..7074)
                     /locus_tag="VFA54_12365"
     CDS             complement(6313..7074)
                     /locus_tag="VFA54_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS51651.1"
                     /translation="MRAVTVFALLFGVRVLLAQHGYSPGEVDEGRRLFLANCAVCHGP
                     EGASVPGTDIGHGKFRRGNTDSDLIQIIRNGIPGTAMPPHSLSEFQAGSIVGYLRFLA
                     DAASDAASSRGNPIRGEAVFRGKGACLSCHRVKGEGSRLGPDLSDIGARRRIAELERS
                     MADPDSEILPQNRFVRLVTADGSAITGRLLNQDAFTVQLFDPQERLLSFPKAGLREFA
                     FLDKSPMPSYKDKLSAQEMEDLVSYLVSLKGIEKP"
     gene            complement(7237..7767)
                     /locus_tag="VFA54_12370"
     CDS             complement(7237..7767)
                     /locus_tag="VFA54_12370"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZS51652.1"
                     /translation="MNRRRAVQTMAAAAAVVVARPARAATPEVDTYVRRWNRAKTFAL
                     AIAEAMPADAYDFKATPAVRAWGPLMVHLASANLNYFRRIKGEAAPLKPPADPEKDKE
                     GVKKFLADSFDWCAGVLGGMSEADLDKSYPGQGKQAALTGRDLVINGFIHTAHTRGYA
                     DVYLRVKGLTPPPYEV"
     gene            complement(7945..8307)
                     /locus_tag="VFA54_12375"
     CDS             complement(7945..8307)
                     /locus_tag="VFA54_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51653.1"
                     /translation="MAIVLKVNGSASGSDRLFASVGGKRFPAPVSITNTGPAAAEVEL
                     RVEAGPTVTIHLAEWRFELPPRGTAETTIEAGAPSSRTGDTVLQAIVDGHVAAEFRLT
                     IVSPARESVFHNLVPGSS"
     gene            8478..8876
                     /gene="queF"
                     /locus_tag="VFA54_12380"
     CDS             8478..8876
                     /gene="queF"
                     /locus_tag="VFA54_12380"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZS51654.1"
                     /translation="MPEYTAEHARAGLDARLPVIETWLNQFRGYQIKIVIPEFTSVCP
                     KTGLPDFGVLTLEYEPDKLCLELKSFKHYILAYRNLGIFYENVVNRVLRDVVRAVRPV
                     SATLVGEFTPRGGLRSTVTASYRRGRKAGR"
     gene            8926..9828
                     /locus_tag="VFA54_12385"
     CDS             8926..9828
                     /locus_tag="VFA54_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZS51655.1"
                     /translation="MASPGLRAAAGPDGRRWRDHMEKFLNAVKPGSREAKLAAAIDPR
                     RLPRHIAIIMDGNGRWARRRGLPRVAGHRAGVRPVRETIESCAQLGIGWLTLYAFSTE
                     NWKRPRAEVDTLWHLLRHSLRKELPELKSNGVRLQIIGRIDCLPEPVQRDLDAAIVET
                     AANNGLRLTIALNYSGRSEIVDALNAILDRCRRHGVFPARLTEESIEANLYTAGMPDP
                     DLLIRTSGEMRISNFLLWQVAYSEIWVTERLWPDFRRLDLLHAIIDYQKRERRYGGLS
                     QAEPVSEELISVSAGAHPGPPPSP"
     gene            9825..10643
                     /locus_tag="VFA54_12390"
     CDS             9825..10643
                     /locus_tag="VFA54_12390"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZS51656.1"
                     /translation="MRRVLTAAVLIPLVIGLIFWGSNRSVRLAFAFLALLCLSEFLRL
                     ASRYGAQPMRLAAYLSGAWVVAAEPIVSIAGAYGPLPALLLDPGPAFFLFLALLLLVL
                     AMLGGRELSASLPGAAATLLGVIYTSIPFRMAADLHALSPRWLFYVLIINWIGDAAAY
                     YAGRAVGRRRLAPRVSPGKTWEGAAASLLTAAPLGTLYLRRFLPAGPGLLPALLLSIV
                     VNAAAQVGDLAESALKRGANVKDSGSLLPGHGGVLDRLDGLLFSVPVVWLAAGR"
     gene            10912..11658
                     /gene="recO"
                     /locus_tag="VFA54_12395"
     CDS             10912..11658
                     /gene="recO"
                     /locus_tag="VFA54_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZS51657.1"
                     /translation="MPARESEALVLRTYPFREADLIVSFIARDQGKLRGVARSARRLK
                     NRFGAGLERLAHVRMFYFQRENRDLVNLDSCELIESQFGVAADYALGVALDFLAEVSE
                     QLLPEHEPNDRYFRLLLLVLDHIRTCGQSGMWAALTYFSLWAVKLGGWLPDLSACVLC
                     GTETPPAAPAFFARSVPGLTCESCRPPNAWALRAESRAAAADMLRVSLRKLSYSAGRS
                     TWADLRQFLLQRLEEQLERKLVTAPLLAGL"
     gene            11701..12630
                     /locus_tag="VFA54_12400"
     CDS             11701..12630
                     /locus_tag="VFA54_12400"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZS51658.1"
                     /translation="MRTFQDIVFDLKRYWARQGCLIQEPYDVEVGAGTMSPETFLRVL
                     GPAPYSVAYVQPSRRPADGRYGENPNRLYKHMQLQVILKPAPADVIELYMKSLEAIGI
                     DLRRHDFKLEEDNWEAPTLGAWGIGWQVMLDGLEITQFTYFQQCGGIDLDVIPVELTY
                     GLERLAAFIQDADSVYDIRWNERWTYRDVRFQEELQFSVYNFEKADVGSLWKAFDLYE
                     AESKRLVDEYRQLRGCDSEEAARRFPLLAAYDGVLKCSHLFNLLDARGAISVTERVAV
                     IGRVRALAVGVAQAWVRQHERARQPASQEAALP"
     gene            12627..14639
                     /gene="glyS"
                     /locus_tag="VFA54_12405"
     CDS             12627..14639
                     /gene="glyS"
                     /locus_tag="VFA54_12405"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZS51659.1"
                     /translation="MRAQDFLLEIGVEEIPDWMIAGALADLRRLFLGVLEKSQLHQGV
                     EISTHATPRRLVLFAKQVPEKQPDSKEIVTGPPASAPEQAVAGFARKMGAEIGQLKKI
                     STGKGEYYSYTRRLKGRRAVDLLGEALPAVILGISWPKSMTWTGKSGPRFIRPIRWLL
                     ALYGGRVVPFELGGVRSDRYTYGHRRLAVERRIRAADFGSFREGLRKNYVLLDAAERR
                     QSIQQGVRRLLGGEQNGLRLRPNEKLLETLVNLTEWPAPILGGFDPEFLSLPEEVLVT
                     VMQHHQKYFSVEDSQRRLAPHFIAVMNLDADPQGFIRHGHERVLRARFNDARFFWETD
                     QKKPLRDRLEDLKHVTFYKDWTYWDKTERNRNLLVNVFGGGELALRANQLAKCDLTTE
                     LVKEFTELQGIVGGLYARAQGEPEEVALAIYDHYQPVPRTETGRVVALADRVETLQSF
                     FGIGLMPTGSKDPFALRRTALSVVRILVEGRRQDRLSRLFEPPEWREFILDRARYYFR
                     EMRGYRYDEVNAVLAAGSENLADAAGRLEALHQVRPTENFEPLATSFKRIKNILIQAG
                     GMECFGSSFDPASLEPGPEQELYAGFQRVAPEVRGLKNERRYLEALIAIASLRPKVDL
                     FFDKVLVNAPDERIRANRLNFLAQLLREFSTIADFSEIVISGEQHS"
     gene            14636..17374
                     /gene="ppdK"
                     /locus_tag="VFA54_12410"
     CDS             14636..17374
                     /gene="ppdK"
                     /locus_tag="VFA54_12410"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZS51660.1"
                     /translation="MTKHVYSFGGGSADGDGKMKDVLGGKGAGLAEMSRANLPVPPGF
                     TISTDVCNLYFASGNRVPEEIEREMSQALEKLEQQMGQRLGDPENPLLVSVRSGAKFS
                     MPGMMDTILNLGLNDQTVESLARKSGNPRFAYDCYRRFIQMFGNVVLEIGKDEFEHIF
                     EGRKKKVKARYDTDLTAEDLKAVIADYKKLVLKKTGKPFPQDARQQLAMARDAVFRSW
                     NNPRAIHYRKMNNIPDDLGTAVNVQAMVFGNLGDTSATGVGFTRNPATGAKEFYGEFL
                     INAQGEDVVAGIRTPKPISELQQIMPEVYEQLRTITSNLEQHYRDVQDFEFTIQDKKL
                     YMLQTRNGKRTGPAAVRIAVDMVEEGLIDKREAVLRVDPMQLDQLLHPVLEEKSRKSL
                     QLLAKGLNASPGAAVGTIVFTADEAVEKAKKGPVVLVRAETVPDDIHGMEVAKGILTS
                     RGGMTSHAAVVARGMGAPCVAGAGAIEVDEARKQMRVNVNGKVVTLREGDWISLDGST
                     GEVYAGQAKTQDPDLTSGHLAKFMSWVDEFRKAGVRANADIPRDAKVARQFGAEGIGL
                     CRTEHMFFAEDRIPHMQAMILARTEKDRRKALARLLPMQRKDFAGLFEAMDGFPVVIR
                     TLDPPLHEFLPKREQLMVDIAVLPKADAKTRKEIASRYARFGAAAGNLKKVLPELLQR
                     VEELHEFNPMLGHRGCRLGISYPEITEMQARAIFEAAVQVAKKGVKVIPEVMIPLVGN
                     VKELENQKQIVVQVANEVFEKAGQSVPYMVGTMIEVPRAAITADKIAKEAEFFSFGTN
                     DLTQTTFGFSRDDSGKFLKVYQDKKIFKDDPFAVLDQEGVGALIHSAVELGRKTRPNL
                     EVGICGEHGGEPSSVAFCVRIGMDYVSCSPYRVPIARLAAAQAALSTTKEEAMRTA"
     gene            complement(17656..19407)
                     /locus_tag="VFA54_12415"
     CDS             complement(17656..19407)
                     /locus_tag="VFA54_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HZS51661.1"
                     /translation="MRRTSLGRQSISRRSFIRSLCAAVPVFSFDQLTAGLPLGVQFVN
                     VAREAGLRAKTIFGSEHKNKYLLETTGCGCAFFDYDNDGWLDIFLVNGTRFEAKWPPG
                     QAPVSRLYKNNRDGTFTDVTLTAGVARSGWGQGVCAGDYDNDGWDDLFVTYWGDCALW
                     HNNGDGTFTDMAPKAGVTTNPGNGKRRWNTGCAFLDYDKDGYLDLFVANYIDFDVKTA
                     PLPESVPCLYKGLVVACGPPGLAGGKNILYHNNGAGVFRDVSEKAGILKTPGTYGLGV
                     LAADFDNDGWPDIYVANDSTSAALYKNNHDGTFTDIAIEAGVAYSPDGKPQAGMGVSA
                     IDYDCDGNLDIVKTNFAGDTSSLYRNSGKMVFEDETFQAGLGKNTRFLGWGVGFVDFD
                     NDGWPDIFICNGHVYPEVGETDSESGYRERKILYRNLGNGKFADVSAEGGPGILEQAP
                     GRGCAFGDFDNDGDIDVLVNCINDLPQLLRCDQGTKNNWLKVKAAGVKSNRSGIGARI
                     YCTTPDGHRQMDEVRSGGSYISQSDFRVHFGLGKAAAAEIEVRWPSGVVDRFRAAANQ
                     TVTVVEGKGMAQGKAPG"
     gene            complement(19418..20677)
                     /locus_tag="VFA54_12420"
     CDS             complement(19418..20677)
                     /locus_tag="VFA54_12420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS51662.1"
                     /translation="MSNPVMGCSSRLRFVRWWLLVASFAPPVCGQGTLKQLEARALEL
                     KSRGDAAGALEAWEKAAALAPQSARIQDEIGFLLEVLHRPEEAIERFERAVELDPRLA
                     AAHYHLGVAYWLRQEAERSIPHLQTAVALEPRNFDYRFHLGHALNETSQFAEALRQLQ
                     AATEINAGNAEAWNQLGLARQNTGDASGAIEAYKRAATLDPANLDARNNLGVMLVNSG
                     HAEQGVAEFQKILKADPSNNMARINIGFAHLQKAELNLAEAEFREVIRREPNLAIAHY
                     DLAVALKQKDDLDGAKAELARTLALDPSLPEAHYTLGIIHWQSGDFSEAAREMQAAIA
                     IRPDYAEAYFMLGTALKQKGEIEGAESALRTAIRLDPSNPGPYNTLAQVLRQKGDVEG
                     SRQAFAQGARAKEKKEAELGKMLQKKQ"
     gene            complement(20770..24210)
                     /locus_tag="VFA54_12425"
     CDS             complement(20770..24210)
                     /locus_tag="VFA54_12425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS51663.1"
                     /translation="MNIWRPLALGFLFLYTPVTSFGQAVYGSIVGTVSDPSNAAVPNA
                     KVTIRDVGKGVAYTTTTNETGNYSQTHLIVGVYEVQVEASGFQTFVQQNVNVSVDAVV
                     QVNARLTVGAVGEVVNVTGEVPLLKTERSDVADTMTQKSLTELPVFSRDMSRLYFLVP
                     GVQATGTTAASEQPQDIYRPRIGGQYWGGIAFLLDGTDNRESVLGEPVITPNLDSVSE
                     LKITTTAYDAEFGQASQAVISAQTKSGSNDFHGSAFEYRRDQHGAARDPFAQARPIPG
                     TNSQFIPPSLWNQFGGSLGGPIQKDKTFFFGDYQGTRQKNGGSLLTRVPTAAERAGDL
                     SDLGVPIWNPCNGSDCNVVPAQRQQFPGAVIPSNLLSPQSQALLKFIPLPNVSGATGA
                     APNYAASGSGIVNWDGFDVRLDRYQTEKLHMFGRYSFLEVNQTVPGAFGFEAGGPNFS
                     TTAFAGAASLRDQSLSYGADYIIRPDWLADFRFGFFRYRVFVNPNGLGTSPAKDAGIP
                     GLNLDNYYTSGMPRFNLNGTGGFQFGYALGVNSCNCPLNEQENEFQWVGNMTHNFGNH
                     ALKFGLDLRFQQNLRVPSDSHRAGELTFDPTTTEGPNGGGLSLASFLLGDVQSFTRYV
                     SNSTDAAERQHRLFSYVQDTWRVTRKLTINYGLRWEIYFPQYVNGKDKGGFQNLATGE
                     VLITGENGIGLNGNVNTALTHFAPRLGIAYQAGPKTVIRTGYGRSYDVGVFGVSFGHN
                     VTQNVPVLANQSLNPSNPWLPVFTLSQGPPALDPNTILASQPKGKTGNPLLPNGINPN
                     VLPLTSSNTMRLPVVDAWNFTVERQITPTIVLSAGYVGNHGYHVTAGGTNYNINQPTI
                     AGFGALTTNQRRLFFQKFGWTQSIKYFADDASVKFNSLQVRGEKRFSNGLMFQGNFTW
                     ASAFDFANDYFFWSHNIDYGREDQVRRFVFNLHHVYELPFGKDKKFLNKSSRGLNYLA
                     AGWQLSGIWLWESGAPFTPSYADCGKDEDTGPCRPNLVGSASVPSPSASGWFATATPG
                     TGGGGCLATATATPELNANGCTRGPWSRPQPGTFGNVARNSFFGPRLFNADASLTKVL
                     PITERVNAQFRAELFNAFNHVNLGQPTATVDSPTAGQIFSIATLSQMRKWQFGLRVNF
                     "
     gene            complement(24569..25069)
                     /locus_tag="VFA54_12430"
     CDS             complement(24569..25069)
                     /locus_tag="VFA54_12430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZS51664.1"
                     /translation="MPDPGIYAHFSTSEGDFSCRLFAEMVPNTVENFVALAEGRKGPQ
                     GKPFYDGLIFHRVIPGFMIQGGCPEGSGRGGPGYRFDDEFHPKLRHNKPGILSMANAG
                     PNTNGSQFFITVAPTPHLDDRHTVFGEVVSGYEVVRKISETPRNRQDRPLKDVVIRQV
                     RIERVA"
     gene            25269..26702
                     /locus_tag="VFA54_12435"
     CDS             25269..26702
                     /locus_tag="VFA54_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HZS51665.1"
                     /translation="MSAGAAPSSKRTFWLQWLLLFAIYAAVQWLLPRPAAVKPEGWRL
                     LGIFLATVAGLMLHPLPGGAIVLIGVTAAALSGALPMAQALGGYANPSVWLVLAAFFV
                     SRALIKTGLARRIALLFVRAFGKSSLGVSYSLILTDVTLASSIPSNAARAGGVVLPIL
                     RSLTEIYGSEPGPTAALLGTFLFTAVYQGECIAAAMFYTGQASNPLAANFAQKVAGVP
                     VTWASWFVAGIVPGVICVLLVPWIILRLLNPRIRHTPEAAKFARRELELMGPPGRAQK
                     IVLAVFVSICGLWLTSSRTRLDITAVALVGACGLFLTGVLTWEDAISERAAWDVFIWY
                     GGLVRLGEALNDFGVTTALAKGIGAYFSGLGWVSLLAVTLLIYFYAHYAFASITAHIL
                     SMFPPFLALLLLRGAPAGLVVYAFACLANLSAGLTHYGTTPSPMFFAHGYVSFRDWWR
                     VGFLVSLVNIAVWCSAGFLWWKVLGIW"
BASE COUNT         4693 a         8694 c         8162 g         5207 t
ORIGIN      
        1 cagcaggctg ttcagcgcca ggccgcccgc caggcacaga ttttctcctg cgccggccag
       61 ttccatcacc accgattcga ccgcccgctg cacgctcgcc gccagatcga gccgcatttc
      121 cggcgtgatc ccctccggcc cttccacccc caggtgggca aaaaattttt cgctgaatcc
      181 gccgtgcgtc ggatggtccg tatcgaaata gctgcggtcg aagctcggcc agccatggga
      241 gtcggcgccc attaccagcc ggaaaaagcc ggcatatttc ggctcgcccg cggccgagag
      301 ccactgcacc ttgtgctctt ccgagcgcgg ccggaacccg agcagcgccg tgatgcgccc
      361 gtaaaaatcc gccggcgaat ccggagcgta tagctcctct tcgagccgca gcgtattgcc
      421 ttccgccctc cacaacgcgc cgcaccgcat gtcgccggtc cggtccagcg tgagcaccgt
      481 cgctcgctcg aagggcgaca tgtaaaaggc gctggccgcg tgggccatgt ggtgatccac
      541 cacccggatg cgcccgttcg ggaacaagga tttcaactgg gtgtggagat gagcggatgc
      601 gccctcgccg agagggccgg cgagaatcac ccaatccacc tcctcgggtt ttgcgtccgc
      661 cagccgcagg cacgtgcgga tggcttccag aggcaggctt cccggctgcc gcgcccagag
      721 cgagccgcgc agcttgtctt cttcaatcgc agcaaccagt gtgccgtccc gcagcacggc
      781 ggcggcggcg tcccgggtca gacccccgat gccgacgatc agcatacgcc attcctcagc
      841 cgtttgatcg cccgctcgcg tccgactgcc gtgaagaccg aaaatgcgct cggcccgacg
      901 ctctgcccgc tcagcgccgc ccgcgccgcg ttgatgatca cgccggcctt caccccgcgc
      961 tccgccgcca aggctcgcag ttcccgctcg atattttcct cggtgaagtc ggggattttt
     1021 tcgagccggt cgcctaactc ccgcagcagc tcgcgcgccc cgggcgcttg cagcttctcc
     1081 tgcgctccgg gctcgatggc gaactggtcg ccgaaatacg ccgccccgcg ccggctgaaa
     1141 tcgcgcaggg tcgtgtagcg cgcccgcagc agatccacag ttttggcaaa ccactcccgc
     1201 tgcccgcccg cataactttc ctgccagagc ccggcgcgcc gcagctcgct ttccacatag
     1261 ggtagcagct cctcaatcgg catcgaccgc agatgctgcg cgttgagcca gagcgctttt
     1321 ggatcgggcc agttttccgg atcgttctcc tgatagctga cgaccgcatt gttgcgattg
     1381 attccttcca gcgaaaacag ctcgatcagc tcccggcggc tgagcacttc acggttgtct
     1441 tttggagacc atcccagcag gctgaggaaa tttacgaaag cgtgcggcaa aaacccggca
     1501 tcgcgatagg ctgtcacgct caccacttgg ccgtggcggc gcttggaaag cttcgaccca
     1561 tcgggagcaa gcagcagcgg caggtgcgcc agctcgggac gctcggctcc cagcccttcg
     1621 aaaatcaaga catgcttgaa ggtgttggcc agatgatcct gtccgcgaat gatgtggctg
     1681 atccgcagat ccgcgtcgtc cgcgcaggac gccagataat aagtcggcgt gccgtcgctg
     1741 cgggccagcg cgaaatcctc gatgtcgccc gtcgagcgcg cctgcggccc gtagacggcg
     1801 tcgtcaaacc ggatctcgct gacccgctcg cgcggcaccc gatagcgcag cacgaaccgc
     1861 tcgccggctg ccgcccgccg gtcgctctcc tcgcggctga tctcccgctg ccccgggttg
     1921 aacagccagg gcgcgccgct gcggtccttg ccggcttcgg cggcctcggc cgccggagtg
     1981 aagtcccggt acgcgaaacc cttatcgagc agcgcctgcg ccgcctgccg gtgcagggcc
     2041 gcccgcgcgg attgggcgta ctcttcatcc cagtcaaggc ccagccatcg caagccctca
     2101 aagatggatt gccgcgatgc ttcggtgttg cgctcgagat cggtgtcgtc gatgcgcaac
     2161 accatcgcgc cgcccttctg ccgggcgaaa agccagttga aaatgtaggt gcgcgcaccg
     2221 ccaatgtgca ggaagccggt gggggatggc gcaaagcgaa cccgtaccat agggagaact
     2281 tcagtatatc aaggggtcgc cggcaagccg ttcgggaact ggctcgcgtg cgattcgagc
     2341 cgcgccgctg ccgccggctg cctgccgggc cggccgcatt tctccatcca accggcgtct
     2401 cctgccgcgg cagcgcccgc ccaagagatc cagccacacg ctgcggccgg actgcacgta
     2461 ccgccttttg aacgcccggc ctagccgccc gcgatagcgc ctatgatcag gaagggctcc
     2521 gctcccgagg cgacctcgtc gggcaatggc gcatccggcg attcgtgcga aaggtcctcg
     2581 gcgcaggcga agaatcgcag aaacggccgg cgctgccggg tggcgtgatc gcggatcgtg
     2641 ccgctcagca tcggatagct cgcctccagc gcatccagaa tggcgcgctg cgtcacctgg
     2701 ccgaccacgt cgagctgcac ttcgtcctcc acctccgcca gagttcgcag atgcggcggg
     2761 agtattactc gtatcatggc aacgtctgaa cctcgacaga aagcaccgcc ggcagatccc
     2821 ggacaatcgg agcccaggtg tctccggcgt cagccgacgc gtagacctgg cccccggtgg
     2881 tgccgaaata taggccgcag gactccagcg agtcaaccgc catcgcgtcg cgcagcacgt
     2941 tcacgtagca gtccttctgc ggcaggcctt tggttagcgc ctcccattcg tttccacccg
     3001 tccggctgcg gaagacgcgc agcttcccct cgggcgggaa atgctctgag tcgctcttga
     3061 tcggaacgac gtatatggtc tccggctcgt gcgcatggac atcgattacg aatccgaaat
     3121 cggtcggcag gttgccgctc acctcccgcc acgattcgcc ggcatcgtcg ctgcgcagca
     3181 cgtcccagtg cttctgcatg aacaacaccc gcgggcgcga agggtgcatc gcaatacggt
     3241 ggacgcagtg gccgacttcg gcgttcggat ccggtatgta ttgcgagcgt aagccgcggt
     3301 tgatcggccg ccacgtttgg ccgccgtcgt cggttcggaa agcgcccgcg gccgagatgg
     3361 cgatgaaaat ccgcccgggg tcgcgcggat cgagaaggat cgtgtgcagg cacatgccgc
     3421 cggcgcccgg ctgccagcgc gggcccgagc cgtggccccg caagcccggg atctcctgcc
     3481 aggtctgccc gccgtcggct gagcggaaca aggcggcatc ctccacgcca gcgtagaccg
     3541 agtccggatc gctcagcgag ggctcgagat gccagacgcg cttgaactcc cacggatgcg
     3601 gtgtgccgtc gtaccactgg tgcgttccgg gaacgccgtc gtacacgaac ttgttgccca
     3661 ccggctccca cgtcgcaccg ccgtcattgg aacgttggat cacctgcccg aaccacccgc
     3721 tggtctgcga cgcatacagc cggttcgggt cggccggcga ccccttgatg tgataaatct
     3781 cccacccccc aaaatgaggg ccgctgatgt cccaccgctc gcgcttgccg tccgacgtga
     3841 ggatgaacgc gcccttgcgc gtgcctacca gtacacgtac cttgctcatg cctcgctcct
     3901 tttccagaat tcactccact ccttccggcg tcgccctcgc gtgccgcgcc ggttgccggg
     3961 cgcctgcgcc gcttttggcc cgcgagtgtt gcgcgcccgc gttgccgaat cgcggcagca
     4021 gaggagttgc atccccttcc tcccagcgcc gccgtacttc ggccaggaac ttctcccggt
     4081 ctttctccac cctctcgatg aaggcgcagg tcgccgccgt tcgttcgtga cgcgcgcccc
     4141 aaatcagcag ttccaggagc acgggcgcca gatcaatccc tttctcggtc agcaggtagc
     4201 tgatccttcg tgcgtcggat tcccgccgcg cctggataat tcccgccgct tccagcctcc
     4261 gcagacggtc tgccagaatg ttggtggcga tcccttcgcc cgattgctgg aactccttga
     4321 acgtccggaa gccgcgaacc atcaggtccc gaatgatcag cagcgaccag cggtccccga
     4381 aaatctccag tgacacgctc accggacagc cggaccggcg tcttgacttc gccttatcag
     4441 gcacgttgga tcgtatcaga atcacttgca gaatgcaagt aaactttcag ggtctgagag
     4501 gtcctcttcg taaccagatt cgaagcgggg ggcgaaacgg attgccggct tggaagggcc
     4561 gtcggcgccg aaacgagtca atcgtcgctt cccgaaagag gttccgccgc acagtcctca
     4621 gcgccggcgc cgagacggga aacggcgttt atcgattgcc gcttgctgtg gcgacttggt
     4681 tttactgccg taacgcaaaa ctgaacagcg cgttcccggc cgccacggcc acgtattgcc
     4741 tgccgccggc caggtacgtc atggggccgg aagcgactgc gccgcccagg ttcgctttcc
     4801 acagcactgc gccggtccgg gcgtccagcg cgtagaaata accctcgcgg ccgccgctga
     4861 aaagcagatc ggaagcggtc gtgagaatgc cggcatcggt cacgtcagtc attttgaatt
     4921 cccacttccg ttccccggtt ctcgggtcaa tggcccgcac cgccccgtag ccttcgtcct
     4981 ctttccggaa attctgcgtg gtgcggccga aaggaatcgg cgatttcgga aatccgccgc
     5041 cgtatctctg cccctcctta aattcctcgg gcgcctttat gtaaatcgag gagtaattgg
     5101 cccaggtagg gatataaaac agcccggtgc gcgggctgta ggacgggttg taccagttcg
     5161 tgccaccctg gttgccggga taaatgaggg tgccttcggt ggttggaact ttcccggcga
     5221 tgcgaatcgg cttccccttc gaatcgaagc cgtccatcca gttcaccgtg acgaagggct
     5281 ttcccatcag gaattccccc gtcgctcggt ccagagcgta gaaaatgcca ttgcggttgg
     5341 caaagaacat cagcttgcgc ggcgttcggc ggccgtcttt gccctgccat tccatatccg
     5401 ccagcacagg cacttgcacg gaatcgtagt caaactcgtc gtgcggtgag aattgataat
     5461 accatttcag cttgcccgtg tccgcgtcca gcgcaacgac ggaatcggta tagaggttgt
     5521 cgcccgggcg cgggtcgccg ttccaatccg gcccgggatt gccgacgccc caatacgtga
     5581 ggttcagctc cggatcgtag gatccggtca cccatatgga accgccccca tgttgccagg
     5641 aatcccctcc ccaggtctcg tgaccgggct cgcccggtcc gggaatggtg tagaagcgcc
     5701 aaacttcttt gccggtgcga gcgttgtagg cggcgatgaa gccccgaatg ccgtactcgc
     5761 cgccggcggt gcctacgatc accttgtctt tcaccaccag gggcgcgtgc gccagagcat
     5821 agcccgactc ggctctggcc accgtcgtat cccacagcgg ctggccgttt ttggcgtcga
     5881 tggcgaccag atgggcatca atagtaccca taaacaacgt gtcccccagg atcgccagcc
     5941 cccgattgat gcggccgcag caggggcggg cttgcggcga gggcgtatac gaataggtcc
     6001 agaagattct gcccgtcgcg gcgtccaggg cgattacgtc attcggcgcc tggacggtgt
     6061 acatcacgcc atccaccacc agggccgtgg cctcgaattt ctccagcgag cgggcctggt
     6121 agatccattg cagttcgagg ttcttcacat tttccggcgt gatctgggtg agcaggctgt
     6181 ggcgctctcc ccgtacgctt ccggaatagg ttagccagtt ctgcggctcc cggtccgcat
     6241 gcaggatgcg gtcgaaggag acctgcgccc aaagagaacc cgcgatggcg gccagcaaaa
     6301 acacgcgtct ggtcatggct tctcgattcc tttcagagac acaaggtaac tgacaaggtc
     6361 ttccatctct tgcgcgctga gtttgtcttt ataggaaggc atgggagatt tatcgagaaa
     6421 agcgaactct cgcaggccgg ctttcgggaa agagagcagc cgctcctggg gatcgaaaag
     6481 ctgcacggtg aaagcgtcct gatttaggag gcggccggtg atcgccgagc cgtccgcagt
     6541 gaccagccgc acaaagcggt tctgcggcag aatttccgag tccggatcgg ccatcgagcg
     6601 ttcgagctcg gcaatccggc gccgcgcccc gatgtcggaa aggtctggac ccaggcgcga
     6661 gccttccccc ttcacgcggt ggcagctcag gcaagcgccc ttgccccgga aaacggcctc
     6721 accccggatg ggattccccc ggctggaagc cgcatccgag gcggcgtcgg ccaggaagcg
     6781 caaatagccg acgatgcttc ccgcctggaa ctccgagagg ctgtggggcg gcattgccgt
     6841 acccggaatg ccgttccgta tgatctggat gaggtcgctg tccgtgtttc cccgtcggaa
     6901 tttgccgtgg ccgatatccg tgcccggaac cgaagccccc tcggggccgt gacaaaccgc
     6961 acaattggcc agaaagagcc ttcggccttc gtccacctcg ccgggactgt agccgtgttg
     7021 ggcgagcaaa accctcaccc cgaaaagaag ggcgaatacc gttaccgccc gcatatccct
     7081 gcctttctac aggcgtcttc gcctgcgcct cgttgcggat gcggccgggc tgccgcgggg
     7141 gcggaggccg gtaaaggcgg cggcgacgcc aatccgacgc aatctttccc ccgcaccttg
     7201 agaaacgcgg cagcctgcca gtgaacggcc ggcccgctac acctcataag gcggcggggt
     7261 gaggcccttt acgcgcaggt acacgtccgc atagccccgg gtgtgcgccg tgtggatgaa
     7321 gccgttgatg accaggtccc ggccggtcaa cgccgcctgc ttgccctggc cggggtacga
     7381 tttgtcgaga tcggcctcgc tcatgcctcc cagcacccct gcgcaccaat cgaaagagtc
     7441 ggcgaggaac ttcttgactc cctctttgtc cttctccggg tccgccggcg gtttcagagg
     7501 cgccgcctcg cctttgatcc ggcggaaata attgagattg gcgctggcca ggtgcaccat
     7561 caacggaccc caggcccgca cggccggggt cgccttgaag tcgtaggcgt cggcgggcat
     7621 cgcctcggcg atcgcgagcg cgaatgtttt cgcccggttc catcgccgca cgtatgtatc
     7681 cacctccggc gttgccgcgc gggctggacg cgccacaacc accgccgcgg cggccgccat
     7741 tgtctgcact gctctgcgtc ggttcatgtc ctcccccctg tacttggaag tgaaagcttc
     7801 cacattttac ctccggggac cccggcgttt tcgcccctgc ttacgttttt tcgaaagccc
     7861 aggtgcgggc tgcccggccc ccacccgccc tccggcagtc ctccgcctcc ggtgccgttc
     7921 cgccgcggcg ccgctccacc acggtcacga gcttcctgga accagattgt ggaacaccga
     7981 ttcccgcgcc ggagacacga tggtcaggcg aaattcggcc gcgacatggc cgtccacaat
     8041 cgcctgcaaa accgtatccc ccgtccggga gcttggggcc cctgcttcga tcgtcgtctc
     8101 cgccgtccct ctgggcggaa gctcgaagcg ccattcggcc agatggatcg tcactgtggg
     8161 tccggcctca acgcgcagct cgacctcggc cgcggccggg ccagtattgg tgatgcttac
     8221 tggagccgga aagcgttttc cacccacaga cgcgaacagc cggtcgctgc cggaagcgct
     8281 tccatttacc ttgaggacaa tcgccatggc gccattatgg catccgccgc gcgatcgccg
     8341 ggacctgcgt cgccgccctc ggcatgcgcc cctgggtggg cctgactccg gcgattgcct
     8401 ccaatttccc gtgctatttt agaaagttga catccgcgga cccgcattgc ggagaaactc
     8461 gtaataaacg gtgacctttg cctgaatata cggccgagca tgcgagggcc gggctcgacg
     8521 cccgtcttcc cgttattgaa acctggctca atcagtttcg gggatatcaa atcaagatcg
     8581 ttattccgga gttcacctcc gtgtgcccga aaaccgggct ccccgatttc ggcgttctga
     8641 ctcttgagta tgagcccgac aagttgtgcc tggaactgaa gtcgttcaag cattacatcc
     8701 tggcctaccg caatctcggc atcttttacg aaaacgtggt gaaccgggtg ctgcgcgacg
     8761 tggtccgggc ggtgcgcccc gtttccgcga cgctggtcgg agaatttacg ccgcgcggag
     8821 gtctgcgttc gacggtcacc gcctcatacc gtagagggcg gaaagcgggc cggtgaagcg
     8881 cgccttgccc gggggttgaa tttgtggcat aaagtgcttt atgatatggc gagtcccggt
     8941 ctgcgcgcag ccgccgggcc ggacgggcgc cgatggcgcg atcacatgga aaaatttctc
     9001 aatgccgtaa agccgggcag ccgggaggcg aagctcgccg ctgcaatcga tccccgccgg
     9061 ctgccccgcc acattgccat tatcatggat ggaaacgggc gctgggcccg gcgccgcggc
     9121 ctgccgcggg tggccggcca ccgggcggga gtgcgcccgg tgcgcgaaac catcgaaagc
     9181 tgcgcccagc taggaatcgg ctggctcacg ctctacgcct tttccaccga gaactggaaa
     9241 cggcccaggg cggaagtcga taccctgtgg catttgctgc gccactcgtt gagaaaggag
     9301 ctgcccgagc tcaaaagcaa cggcgtccgg cttcagatca tcggccgcat cgactgcctg
     9361 ccggaacctg tgcagcgcga cctggatgcg gcgatcgtcg agacggcggc caacaacggc
     9421 ctccggctta ccatcgcctt gaattacagc gggcgctccg agatcgtgga cgccttaaac
     9481 gcgattctcg accggtgccg gcggcacggg gtctttcccg cccggctcac cgaagaaagc
     9541 attgaagcca atctgtacac cgcaggcatg cccgatcccg atttgctgat ccgcacttcc
     9601 ggagaaatgc gcatcagcaa ttttttgctt tggcaggtcg cgtactcgga gatctgggtc
     9661 accgaacggc tctggcccga tttccggcgg ttggaccttc tgcacgcgat catcgattat
     9721 cagaaacggg agcggcgcta tggcgggcta agccaggccg agccggtttc cgaagaactg
     9781 atttccgtca gtgcgggcgc ccatcccggt ccgccgccga gtccgtgaga cgcgttctga
     9841 ccgccgcggt cctcatcccc cttgtgatag ggctgatctt ttggggatcc aaccgcagcg
     9901 tccggctggc cttcgccttc ctcgcgttgc tttgcctcag cgagttctta aggcttgcct
     9961 cgcgctacgg cgcgcagccc atgcggctgg ccgcttattt gagcggcgcc tgggtcgtcg
    10021 ccgccgaacc catcgtttcg atcgccggag cgtacgggcc gctgcctgcc ttgctgctcg
    10081 atccgggccc ggcgtttttt ctgttcttgg cgttattgct gctagtcttg gccatgctcg
    10141 gcggccgtga gctttccgct tcgcttcccg gcgccgccgc cacgctccta ggcgttatct
    10201 acacttcaat tcccttccgc atggccgccg atctgcacgc gctgagcccc cgctggcttt
    10261 tttatgtatt aataataaac tggatcggcg acgccgccgc ctattacgcc gggcgcgccg
    10321 tcgggcgccg caggctcgcg ccccgcgtca gtcccggcaa gacctgggaa ggcgccgcgg
    10381 cgtcgctgct cacggccgcg cctctgggaa cactgtatct gcggcggttc ctgcctgccg
    10441 gccccgggtt gttgccggcg ctgctcctgt cgatcgtcgt caacgcggct gcccaggtgg
    10501 gcgacctcgc tgagtctgcg ctcaagcgcg gcgccaatgt taaagacagc ggcagcctgt
    10561 tgcccggcca tggcggcgtc ctcgaccgct tggacggcct gctgttttcc gtgccggtgg
    10621 tgtggctggc tgccggcaga tgagcccgca tcccagggcg ctgtttcccc aggccgctct
    10681 acaatcggct gcgcggccgc cggcgcaaac gaggcgccgg ctgcttcatc ggggcgcccg
    10741 gccgccgctg ctctgccttc ctcgctgaca actgcgtgcg gagcagggaa cgtgccctct
    10801 gcaaggcgcc gctcgcaacg cggacttgtt ttcccggatc tccgcttgtc tgccgtccgc
    10861 cgcgggcggc tcccaaccgt gttatcctga gtgatcgccg cagacttgtc tatgccggcc
    10921 cgggagagtg aagcgctcgt tttgcgcact tatccgttcc gggaagcgga cctgattgtt
    10981 agtttcatcg cgcgcgatca gggaaagctg cggggcgtgg cgcgcagcgc gcggcggctc
    11041 aagaaccgct tcggcgccgg gctggagcgg cttgcgcacg tccggatgtt ttatttccag
    11101 cgcgaaaacc gcgaccttgt caacctggat agttgcgaac tcattgagtc ccagttcggc
    11161 gtcgcggctg actatgcgct gggcgtggcg ctggattttc tggcggaagt ttccgagcag
    11221 ttgctgccgg agcacgagcc aaacgaccgc tatttccggc ttctgctgct tgtcctcgat
    11281 catatcagga cctgcggcca gagcggcatg tgggcggcgc tcacctactt cagcctgtgg
    11341 gcggtgaagc tcggcggctg gctgccggat ctgtcggcgt gtgttctgtg cgggacagaa
    11401 acgccgcccg ccgcgcccgc attttttgcg cgcagcgtgc cgggcctcac gtgcgagtct
    11461 tgccggccgc cgaacgcctg ggctttgcgc gcagagtcgc gcgcggccgc tgctgatatg
    11521 ttgcgcgttt ccttgcgcaa gctcagctac agcgccggcc gctccacctg ggcggacttg
    11581 cggcaatttc tgctccagcg gctggaagaa cagttggaaa ggaaactggt taccgcgccg
    11641 ttgctggccg ggctgtgaga agcgctttgc gccgcccgcc cacggggctg gaacctttgc
    11701 atgcgaacct ttcaggatat tgtttttgat ctgaagcggt actgggcccg ccaggggtgc
    11761 ctcatccagg agccctatga cgtggaagta ggcgccggca ccatgtcccc ggaaactttc
    11821 ctgcgcgtgc tcggtcccgc tccttacagc gtcgcctacg tccagcccag ccgccggccc
    11881 gccgacggcc gctacggcga gaatcccaac cgcctctaca agcacatgca gcttcaggtg
    11941 attctgaagc ccgcgccggc ggacgtgatc gagctctaca tgaaaagcct cgaggcgatc
    12001 ggcatcgacc tgcgccgcca cgacttcaag ctggaggaag acaactggga agcgcccacg
    12061 ctcggcgcct ggggcatcgg ctggcaagtc atgctcgatg ggctcgaaat tacccagttt
    12121 acgtactttc agcagtgcgg cggcatcgac ctggacgtga tcccagtcga gctgacctat
    12181 ggcctcgagc gcctggccgc cttcatccag gacgcggaca gcgtctatga cattcgctgg
    12241 aacgagcgct ggacctatcg ggacgtgcgc ttccaggaag agctgcaatt ctccgtctac
    12301 aatttcgaga aagccgatgt cggctcgctc tggaaggcct ttgatctgta cgaggccgaa
    12361 tccaaacgtc ttgtcgatga gtatcggcag ttgcgcggct gcgattcgga agaggccgcc
    12421 cgccgcttcc cgctcctggc ggcctatgac ggcgttctga aatgttccca cctgtttaac
    12481 ctgctggacg cccgcggggc gatcagcgtc accgagcgcg tcgcggtcat cggccgcgtc
    12541 cgcgccctgg ccgtgggagt cgcccaggcc tgggtgcgcc agcacgagcg cgcccggcag
    12601 ccggccagcc aggaggccgc cctgccatga gggcccagga tttcctgctg gaaatcgggg
    12661 tcgaggaaat tcccgattgg atgatcgccg gcgcgctggc ggacctccgg cgtctcttcc
    12721 tcggcgtgct cgagaaatcc cagcttcacc agggggtgga aatcagcacc cacgctacgc
    12781 cccgccggct ggtgctgttc gcaaaacagg tgccggagaa gcagccggac agcaaagaga
    12841 tcgtgaccgg gccccccgcc tccgctccgg agcaggcggt cgccggcttc gcccggaaaa
    12901 tgggagcgga aatcgggcaa ttgaaaaaaa tctctaccgg caaaggggag tattacagct
    12961 acacacgccg gctcaaaggc cgccgcgccg tggacctcct cggcgaagcg ctgcccgcgg
    13021 tgattctcgg tatttcctgg cccaagagca tgacttggac cggcaagtcc ggcccgcgct
    13081 tcatccggcc catccgctgg cttctcgccc tctacggcgg ccgggtcgtt cccttcgaac
    13141 tgggcggcgt gcggtccgat cgctatacct atgggcaccg gcggctggcg gtggagcggc
    13201 gcatccgggc cgcggatttc ggcagctttc gcgaaggctt gcggaaaaat tacgtgctgc
    13261 tcgatgccgc cgagcggcgg caaagcatcc aacagggagt ccgccggctt ctgggcggag
    13321 aacagaatgg cctccgcctg cgcccgaatg aaaagctgct ggagacgctc gtcaacctga
    13381 ccgagtggcc ggcgcccatt ctgggcggct tcgaccccga gtttctctcc cttcccgagg
    13441 aggtgctcgt caccgtgatg cagcaccacc agaaatactt ttctgtcgaa gactcccagc
    13501 gcaggctggc tccgcacttc atcgcggtga tgaacctcga cgccgacccg caaggcttca
    13561 tccggcacgg ccatgagcgg gtcctgcgcg cccgctttaa cgacgcgcgc tttttctggg
    13621 agaccgacca gaaaaagccg ctccgcgacc gcctggaaga tctcaagcac gtcacgttct
    13681 ataaggactg gacctactgg gataaaacgg agcgtaaccg caatctgctc gtgaatgtct
    13741 tcggcggcgg cgaactcgcg ctgcgggcga atcaactggc gaaatgcgat ctcaccaccg
    13801 agctggtcaa ggagttcacc gaactgcaag gaatcgtcgg cggtctttac gcccgcgcgc
    13861 agggcgagcc ggaggaggtg gcgctggcca tttacgatca ttatcagcct gtcccgcgca
    13921 ccgaaacggg tcgcgtcgtg gcgctcgcgg accgcgtcga gactttgcag agtttcttcg
    13981 gcatcggctt gatgccgacg ggatcgaaag accctttcgc gctgcgccgg acggctctga
    14041 gcgtggtccg cattttagtc gagggccggc gccaggacag gttaagcagg ctgttcgaac
    14101 cccccgagtg gcgcgagttt atcctggacc gcgcgcgcta ctatttccgc gaaatgcgcg
    14161 gttatcgcta tgacgaagtc aacgcggtgc ttgccgcggg cagcgaaaac ctcgccgacg
    14221 cagccggccg cctggaagcc ctgcatcagg ttcgtcccac ggaaaacttc gagccgcttg
    14281 cgaccagctt caagcgcatc aagaatattt tgatccaggc cggcggcatg gagtgctttg
    14341 gctcctcttt cgaccctgct tcgctcgagc ccggccccga acaggagctc tacgccggct
    14401 ttcagcgcgt cgcgcccgaa gtcagaggcc tgaagaacga acgccgctac ctggaagcgc
    14461 tcatcgcgat cgcttcgctt cggcccaaag tggatctgtt cttcgacaag gtccttgtta
    14521 acgctccgga cgagagaatc cgggccaacc gtctcaattt cctggcgcag ttgttgcgcg
    14581 agttttctac tatcgccgat ttttcggaaa tcgtaatctc aggagaacaa cattcatgac
    14641 caaacatgtt tattctttcg gcggcggctc cgccgatggc gacgggaaga tgaaagatgt
    14701 gctcggcggc aagggcgccg gcctcgctga gatgagccgg gccaacctgc ccgtgccccc
    14761 gggattcacc atctccacgg atgtttgcaa cctctacttc gccagcggga accgcgttcc
    14821 cgaggaaatc gagcgcgaaa tgagccaggc gctcgaaaag ctggaacagc aaatggggca
    14881 gcggctcggc gaccctgaaa atcccttgct cgtgagcgtc cgctccggcg cgaagttctc
    14941 catgcccggc atgatggaca cgatcctgaa tctggggctc aatgaccaga ccgtcgaaag
    15001 cctcgcccga aaaagcggca accctcggtt cgcctacgac tgctaccgcc gctttatcca
    15061 gatgttcggc aacgtggttc tcgaaattgg caaggacgag ttcgagcaca tcttcgaagg
    15121 ccgtaagaag aaggtcaagg ccaggtacga taccgacctt accgccgagg atttgaaggc
    15181 cgtgatcgcc gattacaaga agctggtgct gaagaagacc ggcaagccgt ttccccagga
    15241 tgcgcgccag cagctcgcca tggcgcgcga cgccgttttc cggtcgtgga ataatccccg
    15301 cgctattcac tatcgcaaga tgaacaacat tcccgacgac ctgggcactg cggtcaatgt
    15361 gcaggccatg gtcttcggaa acctcggaga tacttcggcc accggcgtcg gtttcacgcg
    15421 caatccggcc acgggggcca aggaattcta cggcgagttt ctgattaatg cccaaggcga
    15481 agacgtggtg gccggcatcc ggactccgaa gccgatctcg gaattgcagc aaatcatgcc
    15541 ggaggtctac gagcagttgc gtacgattac ttccaacctg gaacagcatt accgggacgt
    15601 gcaggacttc gagttcacga tccaggataa gaagctctac atgctgcaaa cccgcaacgg
    15661 caagcgcacc gggcccgccg ccgtgcgcat cgcggtcgat atggttgaag agggcctgat
    15721 cgataagcgc gaggccgtgc tgcgcgtcga tcccatgcag ttggatcaac tgctgcatcc
    15781 cgtgctcgag gagaaatcgc gcaaatccct ccaactgctt gccaaaggct tgaacgcctc
    15841 cccgggcgct gccgtcggga ccatcgtctt cacggcagac gaggcggtgg aaaaggcgaa
    15901 aaaagggccc gtggtgctgg tgcgcgccga gaccgtgccc gatgacattc acggcatgga
    15961 agtggccaaa ggcatcctca cctcgcgtgg cggcatgacc agccacgccg cggtggtcgc
    16021 ccggggaatg ggcgcgccct gcgtcgccgg cgcgggcgcg atcgaggtgg atgaggccag
    16081 gaagcagatg cgcgtgaacg tgaacggcaa ggttgtcacg ctccgggaag gcgactggat
    16141 ctccctcgac ggctctaccg gagaagtcta cgccgggcag gccaaaacgc aggaccctga
    16201 cttgaccagc gggcatctgg caaagttcat gtcctgggtg gatgaattcc gcaaggccgg
    16261 cgtccgcgcc aacgccgata ttccccgtga cgccaaagtg gcccgccagt tcggcgccga
    16321 aggcatcggc ctttgccgca ccgagcacat gttctttgcg gaagaccgta ttcctcatat
    16381 gcaggccatg attctggccc gcaccgagaa ggaccgccgc aaggccctgg ccaggctgct
    16441 tcccatgcag cgcaaggatt tcgccggcct gttcgaggcc atggacgggt ttcccgtcgt
    16501 tattcgcacc ctcgacccgc ccctgcatga gtttctcccc aagcgcgagc agcttatggt
    16561 ggatatcgct gtgctcccca aggccgatgc caaaaccagg aaagaaatcg cctcccgtta
    16621 tgcgcgtttc ggcgcggcgg cgggcaactt gaaaaaagtc cttcctgagc tgctccagcg
    16681 cgtagaagag ctgcacgagt tcaaccccat gctggggcat cgcggctgcc gcctcggcat
    16741 ctcctatccg gaaataacgg aaatgcaggc gcgggcgatt tttgaagcgg ccgtgcaggt
    16801 ggctaagaag ggcgtgaagg tgattcccga ggtgatgatt ccgctggtcg gcaacgtgaa
    16861 ggagctcgag aatcagaagc agatcgtggt tcaggtggcc aacgaggtgt tcgaaaaggc
    16921 cggccagagc gttccttaca tggtcggcac tatgattgaa gtgccccgcg ccgccattac
    16981 ggcggacaaa atcgccaaag aagccgaatt cttcagcttc ggcaccaacg acctcaccca
    17041 gactacattc ggcttctccc gcgacgattc gggtaagttc ctcaaggtct accaggacaa
    17101 gaaaattttt aaggacgatc ctttcgccgt gctcgatcag gagggcgtcg gcgccttgat
    17161 ccattcggcc gtggaactgg gccgcaagac gcggcccaac ctcgaagtcg gcatttgcgg
    17221 cgagcatggc ggggagccca gttcggtcgc cttctgcgtg cggatcggca tggattacgt
    17281 gagctgctcg ccgtaccggg tccccatcgc gcgcctcgcg gcggcccagg cggccctctc
    17341 caccacgaaa gaagaagcga tgcggacggc ctgaccacgg cctcgccctc cgcggggagc
    17401 gcccatcgtc tcgcggcgct cccatgagtt gcgggcggtg atgcgtgccc ggcaagaacg
    17461 cccgcgggat caggcgtatt cgcggcccca ctgtctttcc gtgcgttcgg gatagccggc
    17521 gtctaggttt acctcgagcg ccggcgaggg atccgcgctt ttggggagag cctccaaccg
    17581 gaagcaggaa aatagaaggg tggatacggg ctccactctc caggcccata gctagttgtg
    17641 atccgctgcg ccgccttacc ccggagcctt tccctgcgcc atccccttgc cctcgactac
    17701 ggtgacggtt tgattcgcgg ctgcgcggaa acgatcgacc acgccgctgg gccaccgcac
    17761 ctcaatttcc gcggcggccg ccttgccgag tccgaaatga acgcggaaat cgctttgaga
    17821 aatgtagctg ccgccgctgc ggacctcgtc catctggcgg tggccatccg gggtcgtgca
    17881 gtaaatccgc gctccgatgc cggaacgatt cgatttcacg ccggccgcct tcaccttaag
    17941 ccagttgttc tttgtgccct gatcgcaccg cagcagttgc gggaggtcgt taatgcaatt
    18001 cactaatacg tcgatgtcgc cgtcgttgtc gaagtcgccg aaggcgcagc cgcgccccgg
    18061 cgcctgctcc agaattccag gcccgccctc ggcagacaca tcggcgaact tgccgttgcc
    18121 cagattccgg tagaggatct tgcgctcccg gtatccggat tcgctgtccg tctcgccgac
    18181 ctcgggatag acatggccgt tgcagataaa aatgtcgggc cacccgtcgt tgtcgaaatc
    18241 cacgaagccc acgccccagc ccagaaagcg cgtgttcttg ccgaggcccg cctggaacgt
    18301 ctcatcctca aagaccattt tccccgagtt tcgatagagg cttgaagtgt cgcccgcaaa
    18361 atttgtcttc acaatgtcaa gattgccgtc gcaatcgtaa tcgatcgcgg aaacgcccat
    18421 gcccgcctgc ggcttgccgt cagggctgta ggccacgccg gcctcgatgg cgatatcggt
    18481 gaacgtgccg tcgtggttgt tcttgtagag cgctgcggag gtggagtcgt tggcgacgta
    18541 gatgtcgggc cagccgtcat tgtcgaaatc ggctgccagc acgccgaggc cgtaagtccc
    18601 cggcgttttc agaatgccgg ccttctcgga aacatcacgg aacacgccgg cgccgttgtt
    18661 gtggtagagg atgttcttgc cgccggccag gccgggcggc ccgcaagcca ccacgaggcc
    18721 tttgtaaagg caggggaccg actccggcag cggcgcggtc ttcacatcga agtctatgta
    18781 attggccacg aacaggtcaa ggtagccgtc tttgtcgtaa tccaggaatg cgcagccggt
    18841 gttccagcgg cgttttccgt tgcctgggtt ggtggtcacg cccgccttcg gcgccatgtc
    18901 ggtaaatgtg ccgtcgccgt tgttatgcca aagggcgcaa tcaccccaat aggtgacgaa
    18961 caggtcgtcc cagccatcat tgtcgtagtc gcccgcgcag acgccctggc cccagcctga
    19021 gcgtgcgaca cccgctgtaa gagtcacgtc ggtgaacgtc ccgtcgcggt tgttcttgta
    19081 aagacggctc accggcgcct ggcccggcgg ccatttcgcc tcgaagcgcg tgccattcac
    19141 caggaaaatg tccagccagc cgtcgttatc gtaatcgaaa aacgcacagc cgcagccggt
    19201 cgtctccagt agatatttgt ttttgtgctc gctgccgaaa atcgttttcg cgcgcaaacc
    19261 cgcctcgcgc gctacgttga cgaactggac gccgagcgga agaccggcgg tcaactggtc
    19321 gaaggagaaa acaggaactg cggcgcacaa cgagcggatg aagctgcggc gggagatcga
    19381 ttgccggccc aaagacgtgc gacgcatgag gttgcggcta ctgcttcttc tgaagcattt
    19441 tacccagttc cgcttccttc ttctccttgg cgcgagcgcc ctgcgcgaag gcctgccggc
    19501 ttccttccac gtcgcccttc tgccgcaaca cctgcgcgag cgtgttatac gggccgggat
    19561 tagaggggtc gaggcggatc gctgtccgca gcgccgactc cgcgccttca atctcgccct
    19621 tctgtttcag cgccgtgccc agcatgaaat acgcttccgc gtaatccggg cggatcgcga
    19681 tcgcggcctg catctctctg gccgcttcac tgaaatctcc gctttgccag tggataatgc
    19741 cgagcgtgta gtgcgcctcc ggcaggcttg ggtcgagcgc gagcgtgcgc gccagctcgg
    19801 ctttggctcc gtcgaggtca tccttttgtt tcagcgccac cgcgagatcg tagtgcgcga
    19861 tcgccaggtt cggctcgcgc cggatgacct cgcggaattc cgcttcggcc aggttcagct
    19921 cggccttctg caaatgggca aatccgatat tgatgcgtgc catgttattg gagggatcgg
    19981 cctttagaat cttctgaaac tccgcgacgc cctgctcggc gtggccgcta ttaaccagca
    20041 tgacccccag attattgcgt gcatccagat tggctggatc cagggtggcg gcgcgcttgt
    20101 acgcttcgat cgccccggac gcgtcgccgg tattctgccg cgccagccca agctgattcc
    20161 aggcttccgc attcccggca ttgatctccg tcgccgcctg caattgccgc agcgcctccg
    20221 caaactgcga ggtttcattg agagcgtggc cgagatgaaa tcggtaatca aaattgcgcg
    20281 gttccaaggc caccgcggtt tgcagatgcg ggatgctgcg ttcggcctcc tgccggagcc
    20341 agtaggcgac gccgagatgg tagtgggccg ccgccagccg gggatcgagc tcgacggccc
    20401 gctcaaaccg ctcgatggcc tcctcgggcc gatggagcac ttcgagaagg aagccaattt
    20461 catcctgaat gcgggcggat tggggagcga gagcggccgc tttttcccac gcctccagcg
    20521 ctccggccgc atcgccgcgc gacttcagct cgagggcgcg cgcctccagt tgcttcaggg
    20581 ttccctgccc gcagaccggc ggcgcgaaac tggcgaccaa aagccaccac cgcacaaagc
    20641 gaagtctgct ggaacagccc ataaccgggt tgctcatagt gttgcatttt tttcgggcgg
    20701 cttccgggtt gcggccgcgc tccaggccgg cgcggctcgc gtttttaggc cttctgttgg
    20761 cgcgcctggt tagaaattta cacgcaaccc gaattgccac ttgcgcatct gggagagcgt
    20821 cgcgatcgaa aaaatctgtc ccgcggtcgg agaatccact gtggctgtag gctgccccag
    20881 attcacgtga ttaaatgcat tgaacaattc ggcgcggaac tgggcgttta ccctctccgt
    20941 gatgggcaaa accttcgtga gactggcatc ggcgttgaag agacgcggcc caaaaaacga
    21001 attccgcgcc acgttgccga acgttcccgg ctgcggacgg ctccagggcc cgcgcgtgca
    21061 gccgttggcg ttcagttcgg gcgtcgccgt cgccgtcgcc aggcaccctc cgccgcccgt
    21121 tcccggcgtc gccgtggcga accagccact cgcactgggg ctcggtacgc tggcgctgcc
    21181 cactaggttc gggcggcagg gaccggtatc ctcatccttc ccgcagtccg cataggatgg
    21241 cgtgaaaggc gcgcctgact cccatagcca gatccccgac agttgccagc cggcggccag
    21301 gtaattcagg ccccgcgaac tcttgttcaa gaacttctta tctttgccga agggcagctc
    21361 gtagacatgg tgcaggttga acacgaatcg gcggacttgg tcctctcggc cgtaatcgat
    21421 gttgtggctc cagaaaaagt aatcgttcgc aaaatcgaac gcgctcgccc aagtaaaatt
    21481 cccctgaaac atcaggccgt tcgagaaccg tttttcgccg cgaacctgga gtgaattgaa
    21541 cttcacgctc gcatcgtcgg cgaagtactt gatcgattgc gtccagccga acttctggaa
    21601 aaataaccgg cgctggttcg tggtcagcgc cccgaagccg gcgatagtcg gctggttgat
    21661 gttgtaattg gtgccgccgg cagtcacatg atagccgtgg ttgccgacgt agccggccga
    21721 cagcacgatc gtgggcgtga tctggcgctc cacggtgaag ttccaggcgt ccaccactgg
    21781 caggcgcatc gtgttgctgc tggtgagagg cagaacattc gggttgatcc cattcggcag
    21841 cagcgggttt cccgtcttgc ctttcggctg cgaggccaga atcgtgttgg gatcgagtgc
    21901 cggcgggcct tgcgaaagcg tgaatacggg aagccacgga ttggaaggat tcagcgactg
    21961 gttcgccagc accggcacgt tttgggtcac attgtggccg aaggatacgc caaacacgcc
    22021 cacgtcgtac gaccgcccat acccggtccg gatcacggtc ttggggcccg cctgataggc
    22081 gatgcctaac cttggcgcaa agtgggtgag cgcagtattc acgttcccgt tcaggccgat
    22141 gccgttctcg ccggtgatca gaacctctcc cgtggccagg ttctgaaagc cgcccttgtc
    22201 cttgccgttc acatactgcg ggaagtaaat ctcccaccgc aggccgtagt tgatcgtcag
    22261 cttccgggtg acgcgccagg tgtcctggac ataggaaaac agacggtgct gccgctcggc
    22321 cgcgtccgtc gaattgctca catagcgggt gaaggactgc acatccccga gcaggaacga
    22381 agcaagcgaa aggccgcctc cgttcggccc ttcggtcgtg gttgggtcaa atgtcagttc
    22441 gccggcccgg tgcgagtcgc tcggcacgcg caggttctgc tgaaaacgca agtcgaggcc
    22501 gaacttcaga gcatggttcc cgaagttgtg cgtcatgttg cccacccact gaaactcgtt
    22561 ttcctgctcg ttcagcgggc agttgcacga gttaacgccg agcgcgtatc cgaattgaaa
    22621 gccgcccgtg ccgttcagat tgaaacgagg catgcccgag gtgtagtagt tgtccaggtt
    22681 caaacccgga atcccggcgt ctttggccgg agacgtgcct agcccgttgg ggtttacgaa
    22741 aacgcgatag cggaagaaac cgaaacggaa gtccgccagc cagtccgggc ggatgatgta
    22801 atcagcgcca taagacaggc tttggtcgcg gagcgaggcc gcgccggcga acgctgtggt
    22861 gctgaagttc ggcccgccgg cctcgaatcc gaatgccccg ggtactgtct ggttgacttc
    22921 caggaagctg tagcggccga acatatgcag cttctcggtc tgatagcggt ctagccggac
    22981 gtcaaagcca tcccaattga caatgccgct tcccgaggcg gcatagttgg gggccgcccc
    23041 ggtggcgccc gagacgttcg gcagcgggat gaacttgagc agggcttggg attgtgggga
    23101 gagcaggttg gacgggatca cggccccggg gaattgctgc cgctgcgcgg ggacgacatt
    23161 gcaatccgag ccgttgcagg gattccagat gggtacccca agatcgctta agtctccagc
    23221 ccgttccgca gccgtgggca cccgcgtcag cagcgagccg ccgttcttct ggcgcgttcc
    23281 ctgataatca ccgaagaaga aagtcttgtc tttctgaatc ggccccccca gcgagccccc
    23341 aaactggttc cacaggcttg gagggatgaa ctggctgttc gtgccgggaa tgggccgggc
    23401 ctgcgcgaac ggatcgcgcg ccgcgccatg ctgatcgcgc cggtattcga aggcgctgcc
    23461 gtgaaaatcg ttgctccccg acttcgtctg ggcggagatc accgcttgac ttgcttgccc
    23521 gaattctgcg tcgtaagcgg tggtggttat cttcagctcc gagaccgaat ccaaattcgg
    23581 agtgatgacc ggctcgccca aaacggactc ccggttgtcc gtgccgtcca gcaggaaggc
    23641 gatgccgccc cagtactgcc cgccgattct gggccggtag atgtcttgcg gctgctcgct
    23701 ggccgccgtg gtgccggtcg cctgcacgcc gggaaccagg aagtacaacc ggctcatgtc
    23761 gcgcgagaac accggcagtt cggtcagcga cttttgcgtc atcgtgtcgg caacgtcgct
    23821 ccgctcggtt ttcagcagcg gaacttcgcc cgtgacattg acgacttcgc cgaccgcgcc
    23881 gacagtcagc cgcgcgttca cctgtacaac ggcgtccacg ctgacgttca cattctgctg
    23941 gacgaaagtc tgaaaaccgg aagcctcgac ctgcacctcg tacacgccca cgatcaaatg
    24001 ggtctggctg tagtttccgg tttcatttgt ggtggtagtg tacgccacgc ctttgccgac
    24061 atcgcggatc gtcaccttgg cattgggaac agcggcattc gaagggtcgc tgactgtccc
    24121 cacaatgctt ccatagacgg cctgcccgaa tgacgtgaca ggtgtgtaca aaaatagaaa
    24181 ccctaaggcc agggggcgcc aaatgttcac aggtccctcc ttcaccaaag gaatttattc
    24241 gaaatgcccg tagtatacac ctgcgagccc cggttgcaag cggattttta aagcaatgcc
    24301 gaagcctttt ttttgccttc ggatcgtatt tccgtggccg aaacgggccc gccgcctccg
    24361 caaagcggat tttctcgttt cggcttgccg gaacccgcgg catcacgccg ccttctccgt
    24421 tagcgttcgc cactccagaa ggcggacgga tcgggcttcg tcttgggtcg ttgctggaat
    24481 tagctggcac gagtgccggg aaggcgctcg attttcagtt cggctatcgg gtcccgggcc
    24541 tccggcaagc tgcggcgcgc ggccagcctc aggccacgcg ctcgatccgg acctgccgga
    24601 ttaccacatc cttcagcggc cggtcctggc gatttcgcgg cgtttcggag atcttcctca
    24661 cgacttcgta gcccgaaacg acctccccga aaacggtatg ccggtcgtcg aggtgcggcg
    24721 tgggcgccac ggtgataaaa aactggctgc cgttcgtatt cgggccagcg ttcgccatgg
    24781 acagaatgcc cggcttgttg tggcgcaatt tgggatgaaa ctcatcatcg aagcggtagc
    24841 ccgggccgcc gcggcccgat ccctccggac accctccttg gatcataaag cccggaatga
    24901 ccctgtgaaa aatcagcccg tcgtaaaacg gcttgccttg gggccctttg cgcccttcgg
    24961 cgagagccac aaagttctcc accgtattgg gaaccatctc ggcgaacaag cggcaactga
    25021 aatccccctc gctcgtcgaa aaatgagcat agatgcctgg gtccggcatt gtcggaaaca
    25081 tccctccaac gccagcgtac catgccgggc tccgtctccg gacgtcctgc ctggcgccgc
    25141 cggcgatctt cggcaggtaa ttattcagga cacagccgct gtccccacag tcagccttcc
    25201 gcgcgataag ccctctcatt cctccatccg cggcccgccg gcccctgttc tgctagaata
    25261 cgcccttcat gtcggccggg gcggctcctt ccagcaagcg aacattctgg ttgcaatggc
    25321 tgttgctttt cgcgatttat gctgcggtcc agtggctgct gccgcgcccg gcggccgtca
    25381 agccggaagg ctggcgcctg ctgggcatct tcctggcgac tgtcgccggc ttgatgctcc
    25441 acccgctgcc gggcggagct atcgtgctca tcggggtcac tgccgccgcg ctcagcggag
    25501 cgctgccgat ggctcaggcg ctgggcggtt atgccaatcc gagcgtctgg ctggtgctgg
    25561 cggccttttt tgtttcgcga gccctcatca aaaccggcct ggcgcgccgc attgcgctct
    25621 tgtttgttcg ggcatttgga aaaagctcgc tgggcgtttc gtattccctc atcctgaccg
    25681 acgtgaccct cgcctcctcg attccttcca acgcggcccg cgccggcggc gtcgtccttc
    25741 cgattctgcg cagccttacc gaaatctacg ggtccgagcc gggccccacc gccgcgctgc
    25801 tcggcacgtt cctgttcact gccgtgtacc agggagaatg tattgctgcg gccatgttct
    25861 ataccggaca ggcgagcaac ccgctggcgg cgaacttcgc ccaaaaagtg gcgggagtcc
    25921 ccgttacctg ggcgagttgg ttcgtggcgg gaatcgtgcc gggagtcatt tgtgtcctgc
    25981 tcgtaccctg gatcattctc cgcctgctca atcccaggat ccgccatacg cccgaggcgg
    26041 ccaagttcgc ccgccgcgag ctcgagctga tggggccgcc cggccgcgcc caaaaaatcg
    26101 tgctggccgt gttcgtcagc atctgcggcc tgtggctgac cagcagccgg acccgcctgg
    26161 atattacagc cgtcgccttg gtcggcgctt gcggcctttt cctcaccggc gtgctcacct
    26221 gggaagacgc tatctccgag cgcgcggctt gggacgtgtt catctggtat gggggattgg
    26281 tccgcctcgg cgaggcgttg aatgacttcg gcgttacgac tgctctcgcc aaaggcatcg
    26341 gggcctattt cagcggcctg ggctgggtgt ctttgctcgc cgtgacgctc ctgatttact
    26401 tttacgccca ctacgccttc gcttcgatta cggctcatat cctttccatg ttcccgccat
    26461 ttctggcgct cctgctgctc cggggagccc cggcggggct cgtagtttac gccttcgcct
    26521 gcctggccaa cctctccgcc ggcctcactc actacggcac tacgccgagc ccgatgtttt
    26581 tcgcccacgg ctacgtcagc ttccgtgatt ggtggcgggt ggggttcttg gtgtccctgg
    26641 tgaacattgc tgtttggtgc tcggctggct ttttgtggtg gaaggttttg ggtatctggt
    26701 aagccggccc gctcgccaag ctgttcccag acttccccgg aatctcccgc cacatc
//
LOCUS       DASREK010000236         5936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2260252, whole genome shotgun sequence.
ACCESSION   DASREK010000236 DASREK010000000
VERSION     DASREK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260252"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..2160)
                     /gene="argS"
                     /locus_tag="VFA54_12440"
     CDS             complement(172..2160)
                     /gene="argS"
                     /locus_tag="VFA54_12440"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZS51666.1"
                     /translation="MFLILEQRMRDAFAARIREAYSLDLPVVIEQPREPQFGELAVPV
                     AFSLARRLRQPPRRIAEELVAAMPPIPGVAGLEVAGNGYINVRFDRGAYGSALLRGRT
                     AEGRRAPGRDSPTAAVPAGKIVVEHTNINPNKAAHIGHLRNAVLGDTFVRMLRARGRQ
                     VEVQNYIDNTGVQVADVVAAFYRLENKTPEQVQRLIGDPAIRFDYYCWDLYARISRHY
                     EEHPESLQWRAETLHAIEAGAGPLARIAHLVADAIVLAHLKTMLRLGIQYDVLPRESE
                     ILHLRFWATAFELLKERKAIYLETQGKNAGCWVMPAEAFRGEGENSEESKVIVRSDGT
                     VTYVGKDIAYQLWKFGLLGRDFLYRLLLTYEDGRGLWVTTDEPQNDPSPLFGHATRVY
                     NVIDVRQAYLQQVVKAGLRALGFEREAENSIHFAYEMVALSPRCCADLGIELSDEDRQ
                     RPYVEVSGRKGLGVKADDFIDALIEKALDEVSMRHPHAAPEYQRAIATRIAVGALRYF
                     LLKFTRTSVIAFDLQEALSFEGETGPYVQYAGVRARNILNKLRERGETLPDFSAELQD
                     EAFTRQLQSEDFWQVLLGASQADAVVEKAVDAGEPAHVARYAFQLAQAFNNFYHQHHI
                     LSETDREKRTFLLWMTQFFADQLAYVLSILGIETPEVM"
     gene            complement(2300..3262)
                     /locus_tag="VFA54_12445"
     CDS             complement(2300..3262)
                     /locus_tag="VFA54_12445"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS51667.1"
                     /translation="MYRRVFSVTALAVLPALLLAQNPKKYVASAHVVSQVRRAAWAND
                     LPAAERALADYRAQHPELTPDLVEAISWAARGASFVKNWEKAELYAKQAFDGSQKLLE
                     NRKLDDDKSVPIALGAAIEVLAAVQDARGDRAGALEFLNEQYQRYKGTSIETRIQKNI
                     LLLSLEGKPAPAIAMERWIGEKPKTLAELKGKVVLLFFWAHWCGDCKAEMPVLARIQQ
                     DFGGKGLTIVGPTQLYGYIDHGKDATPDQEMEYLQNEYQKQHPIPAWMRVPVSQENFL
                     KFGVSTTPTLMLVDRRGIVRLYHPGNLSYEELAAKITPLLEPRT"
     gene            3336..4190
                     /locus_tag="VFA54_12450"
     CDS             3336..4190
                     /locus_tag="VFA54_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS51668.1"
                     /translation="MKRAAWIRLGALALAVGGVWIALTQQQPPADLTVEKIADDLHVL
                     IGSGGNVAVYTTDEGVILVDDKFEQNYPQILAKVKSLTDKPIRYVLNTHQHGDHTGGN
                     AKMLAASVEILAHENARANMIRNKQPGPPRIAFADEVSVTLGGKTVQARHFGAGHTNG
                     DAVMYFPALRVVHTGDLFVRGSPFIDYDNGGSSDAWMKTIDGILTLDFDTVIPGHGAV
                     SKRADLIAWKADFEKLRSRVREMTRQGKSRGEVATLLKVDDLPGWTMGGLFAKSLPAL
                     YDEMARSR"
     gene            4379..5206
                     /locus_tag="VFA54_12455"
     CDS             4379..5206
                     /locus_tag="VFA54_12455"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS51669.1"
                     /translation="MKPLTRLADLKAKHWGQSNWSELVILDNNTRAYVISAAPNSRVS
                     RRLYADSPAWWVVQEGRIRFEIEDDNGKFQTFEARKGSYVFAAERHLFALEVIGDQPA
                     IRFEVTLADATPVFDAKPEGAGSGEIEYIPVTLDTGRNPLDVPNPGGKPDRIHVNIED
                     LEAAHKGQSAWSEPAMRKNRVRGNFIYGHTSDNPRHPPGYRGHMHADFAEFWIVLRGQ
                     LTWKLEGIEKPILAGEGDIVYAPPKTFHEPEFYGSGPACRLTSSTYPSANHIYDAPQ"
     gene            complement(5358..>5936)
                     /locus_tag="VFA54_12460"
     CDS             complement(5358..>5936)
                     /locus_tag="VFA54_12460"
                     /inference="COORDINATES: protein motif:HMM:NF017538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HZS51670.1"
                     /translation="RRFAELGVPVDLPDLSGGDFEHLTLTSQLAVIGRRARELKPRAV
                     IGSSLGGYLAVLYAARNSGAFPALVLLAPGFGFARRWPQSLGEDVLRAWRERGWMEVY
                     HYGLRRPCRLDFRLVEDGANYEDYPDVREPVLIVHGTRDDVVNPLWSQEFAASRPNVT
                     LRLVDSDHQMLDALDLIWEMVRKFLEDHQLLP"
BASE COUNT         1159 a         1836 c         1810 g         1131 t
ORIGIN      
        1 cccgccgtat tcaccgcggc cgacggccgg tgcaatctac atgcggctgc ggctatagcc
       61 ctgcccctcc cggcggtcta tttcgcgggc gttgagggcg agcccgggtg ctccttggaa
      121 ttcctttccg cggagcccga gcttccgctc gtctgcggga ggaaggcggc cctacatcac
      181 ttcgggcgtc tcaattccga gaatgcttag aacgtaggca agctgatcgg caaagaattg
      241 ggtcatccac agcaggaagg tgcgcttttc gcgatccgtt tcgctgagga tgtggtgctg
      301 atgataaaaa ttattgaacg cctgcgcgag ttgaaaggcg taacgcgcca cgtgcgcggg
      361 ctcgccggca tccaccgctt tttcgactac cgcgtcggcc tgcgaggcgc caagcagcac
      421 ctgccagaag tcttcgctct gcaactgccg ggtgaacgcc tcatcttgga gttccgccga
      481 aaagtcgggc agggtctcgc cgcgctcgcg gagtttgttg aggatatttc gcgcacgcac
      541 ccccgcgtat tgcacatacg ggccggtttc gccttcaaag ctgagcgcct cctggagatc
      601 gaaggcgatc accgacgtcc gcgtaaattt caacagaaaa taccgcagcg ccccgacagc
      661 gatgcgtgtg gcgatcgcac gctggtattc gggagcggcg tggggatgcc gcatggagac
      721 ttcgtccagc gctttttcaa tgagcgcgtc gataaagtca tcggctttca ccccgaggcc
      781 tttgcgtcct gacacttcga catacggccg ctgccggtcc tcgtcagaaa gctcgatccc
      841 caggtctgcg cagcagcgcg gcgacagggc gaccatttcg taggcgaagt ggatggagtt
      901 ttccgcctcg cgctcgaagc caagcgcccg cagcccggcc tttaccacct gctgcaaata
      961 ggcctggcgg acgtcgatca cgttgtatac gcgcgtggcg tgcccaaaca agggagatgg
     1021 atcattttgc ggctcgtcgg tcgtcaccca gaggcctcgc ccgtcctcat aagtgagcag
     1081 cagccggtat aggaaatcgc ggccgaggag cccgaacttc cacaactggt acgcaatatc
     1141 cttgccgacg taggtcacgg tcccgtcgga gcgcacaatg accttggact cctcgctgtt
     1201 ctcgccttcg cctcgaaaag cttcggccgg catcacccag cagccggcgt ttttgccctg
     1261 ggtttcgaga taaattgctt tacgctcctt cagcagctcg aacgccgtgg cccagaagcg
     1321 taaatgcaaa atttcgcttt cgcggggcag tacatcgtat tggatgccca gccgcagcat
     1381 ggtcttcagg tgagccagga caatggcgtc ggccacgaga tgggcgatgc gcgccagtgg
     1441 gccggcgccc gcctcgatgg cgtgcagagt ttcggccctc cattggaggg actcgggatg
     1501 ttcctcgtag tgccgggaga tccgggcgta caagtcccag caataataat cgaagcgaat
     1561 ggccggatcg ccgatgagcc tctgcacctg ctccggcgtc ttgttttcca gccggtaaaa
     1621 ggcggctacg acgtcggcga cctggacgcc ggtgttgtcg atatagtttt gcacctcgac
     1681 ctggcgcccg cgcgctcgca gcatgcgcac aaacgtgtca ccgagcactg cattccgcaa
     1741 atggccaata tgagccgcct tgttcgggtt gatattggta tgctcgacga caatcttgcc
     1801 ggcggggacc gccgccgttg gggaatcccg gcccggagca cggcgtccct ccgcggtccg
     1861 gccgcggagc agcgcggacc catatgcgcc gcggtcaaac cgcacgttaa tgtagccgtt
     1921 gccggcgacc tccaggccgg caacgccggg tatgggcggc attgcggcca caagctcttc
     1981 ggcgatccgg cgaggcggct ggcgaagccg gcgcgccagc gaaaaggcga ctggtacggc
     2041 gagctctccg aattgcggct cgcggggctg ctcgataacc accggcagat cgagcgaata
     2101 cgcttcgcga atgcgggcgg cgaaggcgtc ccgcatcctt tgttccagaa ttaaaaacat
     2161 ggtccggcag gttgctgcat agggacggcc gcttcctcac ggccgaggct cgaaaactcg
     2221 ctctctcaat tttgaagctt attccaaagg atacgccgag ccgcgcgcgt cagaaagcgg
     2281 gggagccctc ggcagccttc taagttcttg gctccagcaa gggcgtaatt ttggccgcca
     2341 gctcttcgta ggaaaggttg cccggatgat acagccggac aatcccccgg cggtccacca
     2401 gcatcagggt cggagtcgtg cttacaccga acttcaggaa attttcctgg ctcaccggca
     2461 cccgcatcca ggccgggatg gggtgctgtt tttgatattc gttttgcagg tactccattt
     2521 cctgatccgg ggtggcgtct tttccgtggt caatgtagcc gtaaagctgg gtcggcccaa
     2581 caatggtcaa acccttgccg ccgaaatcct gctggatgcg ggcgagaacc ggcatttcag
     2641 ccttgcagtc gccgcaccag tgcgcccaga agaagaggag cacgaccttt cctttcagtt
     2701 cggcaagagt cttcggtttt tctccgatcc aacgctccat ggctatggcg ggcgccggct
     2761 tgccttcgag gctcagcagc aagatgtttt tctggatgcg ggtctcaatc gaggtccctt
     2821 tgtaccgctg atactgctca ttgagaaatt ccagagcccc ggcgcggtcg ccccgggcgt
     2881 cttgcaccgc cgccagcacc tcgatggcag ccccgagcgc gatgggaacg cttttgtcat
     2941 cgtcgagctt tcgattttcg agcagcttct ggcttccgtc gaacgcctgc ttggcataaa
     3001 gctcagcctt ctcccagttc ttcacgaagc tggcgccccg cgcggcccac gagatcgcct
     3061 cgaccagatc gggcgtcaat tccgggtgtt gggctcggta gtcggccagc gccctttcgg
     3121 ctgccggtaa atcattggcc caagccgccc gccgcacctg cgaaacaacg tgggcgctgg
     3181 cgacgtactt tttggggttc tgggcgagca gcaacgcggg cagaacagcc agagcggtaa
     3241 cggaaaatac acgtcgatac atacccacag catacatctt tttctttggc ccggcgcgga
     3301 tttcggttag aatcagacaa ctcgggaggt ccgatatgaa gcgtgcagca tggatccggc
     3361 tgggcgctct ggccctggcg gtaggcggcg tctggatcgc cttgacccag cagcagcccc
     3421 ccgccgatct gacggtcgag aagatcgccg acgatctcca cgtactgatc ggcagcggcg
     3481 gcaacgtggc cgtctatacc acagacgaag gcgtgatcct ggtcgacgac aagtttgagc
     3541 aaaactatcc tcagatcctc gctaaggtca aatcgctaac cgataaaccg atccgctacg
     3601 tcttgaatac ccaccagcac ggcgaccaca cgggcggcaa cgccaagatg ctggctgctt
     3661 cggtcgagat tctggcgcat gaaaatgccc gcgccaatat gatccgcaac aagcagccgg
     3721 gcccgccgcg catcgctttt gcggacgaag tttcggtgac tcttggcggc aaaaccgtcc
     3781 aggcgcggca ttttggcgcc ggccacacca acggcgatgc cgtcatgtac tttccggccc
     3841 tacgtgtggt ccacaccggc gatctgtttg tacgcggcag cccgttcatc gactacgaca
     3901 atggcggcag ctccgatgct tggatgaaga ccatcgacgg tattctgacc ctcgatttcg
     3961 ataccgtaat tcccgggcat ggagcggtct cgaagcgtgc ggatctgatc gcttggaaag
     4021 ccgatttcga aaagctgcgc agccgcgtcc gggagatgac ccggcaaggc aagagccgcg
     4081 gcgaggtggc cactctactg aaggtagacg acctgcccgg ctggaccatg ggcggcctct
     4141 tcgccaaaag cctgccggct ctctacgatg agatggcaag gtcgaggtaa gcgaaatgga
     4201 accgcccacg ggctctgctg gcgctcgcag atgttggtgt aggggcagcc gccggcgctg
     4261 gccggcgctc atctgcggct gcctactgct gtgcgcatgg gcctacgggc agagcgcgcg
     4321 gccgaaacgt gttttttact tcccgaagcc ggttcagccg actccctaca agccgccgat
     4381 gaagccgctg acgcgcctgg ccgatctgaa ggccaaacat tggggtcaaa gcaactggag
     4441 cgagctggtc attctcgata acaatacccg cgcctatgta atttcagcgg cgccaaattc
     4501 ccgggtttcc cggcggctat atgcggactc gcccgcctgg tgggttgtgc aagaagggcg
     4561 gattcgtttc gagatcgaag acgacaacgg caaattccaa accttcgagg cgcggaaagg
     4621 ctcctacgtc tttgccgccg agcgccactt gtttgcgctc gaagtgatcg gagatcagcc
     4681 cgctatccgg ttcgaagtca cgctggctga tgcgacgcct gttttcgatg cgaagccgga
     4741 gggcgccggc agcggcgaga ttgaatatat tccggtcact ctcgataccg gccggaatcc
     4801 gctggatgtt cccaatccgg gcggcaagcc ggaccgcatc cacgtgaata tcgaagacct
     4861 cgaggcggcg cacaaaggcc agagcgcgtg gagcgagccg gccatgcgca agaaccgcgt
     4921 gcggggcaac ttcatctacg gccacaccag tgacaatccc cggcatcctc ccggttatcg
     4981 aggccatatg cacgcggatt ttgcggagtt ctggatcgtt ctacgcggcc agttgacgtg
     5041 gaagcttgag ggcatcgaga agccgattct ggccggggaa ggcgacatcg tctatgcccc
     5101 gccgaagacc tttcacgagc ctgagtttta cggcagcggc ccagcctgcc gtctcacgag
     5161 cagcacctat cccagcgcaa atcatatcta cgacgcgccg cagtgatgag ccgcggaaaa
     5221 aacgcgccgc gaaaatacga cttggaagat ggtcgaagtg agcagaatca attcccgggt
     5281 cggtccgccc tccgataccg gagcgccggc agggcggatg atctgcggat gatctgcggc
     5341 ccggcgccga ggttccgtca gggcagcaac tggtgatcct ccaaaaactt cctcaccatc
     5401 tcccatatca ggtcgagggc gtccagcatc tggtggtcgg aatcgaccag tcgcaaggtg
     5461 acattcgggc ggctggcggc gaattcctgg gaccagagcg gatttacaac gtcgtcgcgg
     5521 gtcccgtgaa cgatcaacac cggctcgcgt acgtccggat aatcctcata gttcgcgccg
     5581 tcttcgacga gccggaagtc aaggcggcaa gggcgcctta gcccatagtg gtaaacctcc
     5641 atccagccgc gttcgcgcca ggcgcgcaac acgtcctcgc ctaagctctg gggccagcgg
     5701 cgggcgaacc caaatcccgg cgcaagcagg accagggcgg ggaacgcgcc agaattccgg
     5761 gcggcgtaga gcacagctaa ataacctccc agactcgagc cgatcacggc gcgaggcttc
     5821 agttcccgag cgcgccggcc gattaccgcg agctggctgg tgagggtgag gtgctcgaaa
     5881 tcgccgccgc tcaaatcggg cagatccacc ggcacaccaa gctcggcaaa gcggcg
//
LOCUS       DASREK010000237         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_940055, whole genome shotgun sequence.
ACCESSION   DASREK010000237 DASREK010000000
VERSION     DASREK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940055"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..612)
                     /locus_tag="VFA54_12465"
     CDS             complement(250..612)
                     /locus_tag="VFA54_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS51671.1"
                     /translation="MPAARNGKICYIEIPATDIASSAEFYRRVFGWRIRTRTDGRTAF
                     DDITGEVSGSWVLGRAPAGEPGLLIYVMVDSVAATLDAIAASGGQIVQPIGADAPEIT
                     ATFHDPAGNLVGLYQETS"
     gene            complement(654..896)
                     /locus_tag="VFA54_12470"
     CDS             complement(654..896)
                     /locus_tag="VFA54_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZS51672.1"
                     /translation="MKPIACYMEETGMSLEQLVAAAGLDAKLVKAIVSGNYTPSPAQR
                     QRLAAALGLSQGDIAWGHAIPVQHLRGNGPQCGRST"
     gene            1058..>2326
                     /locus_tag="VFA54_12475"
     CDS             1058..>2326
                     /locus_tag="VFA54_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZS51673.1"
                     /translation="MIAEPAGCWLSRFLFERLLALIYLIAFLCAANQFVPLLGKDGLL
                     PAPYFVRRMRFRASPSLFFFAPTDRAFRIAAWAGVALSCFAAAGYSEKYGVLVSAAVW
                     ASLWVLYLSFVNVGQTFYAFGWESILLETGFFTIFAGGGATAPSAVLCWIWRWVLFRV
                     MFGAGLIKLRGDSCWRELTCLDYYFETQPIPNPLSWHFHWLPSWVHRAGVLFNHFVEL
                     VVPFAYFAPQPVSAAAGLITIAFQFTLILSGNLSWLNWLTVTLAVSTLDDRWLSWIPV
                     TPPSLHPAYAAHRAAIYALAVMVAVLSVRPVANMLSRHQLMNYAFNRFHLVNTYGAFG
                     GITRTRYEIVIEGAEDPDPGPATQWREYEFKGKPGDPARLPPQIAPYHLRLDWLMWFA
                     AMGSAYEHPWLINLAAKLLEGDRHIVKLLKK"
BASE COUNT          391 a          732 c          687 g          516 t
ORIGIN      
        1 gctatagagg caggcattta cgatgccgaa gaggaggata gcgttgcgga ttcgcaccag
       61 caagcgcagt gtagcacgca gccggcttgt tgtgcggaat aggagtcagc tcttgaaggc
      121 gccggtcagc ggacaaacct cgattcgcaa agaaccggcg gaaagcagtc acatttcgaa
      181 aacagaggcc gcaaatctcc gccgagcagc tctcgattct gtgggcgctg ttcctccggc
      241 ccgcccggtt caggaggtct cctggtagag gccgaccaga ttaccggccg gatcgtgaaa
      301 tgttgcggta atctccggcg cgtccgcgcc aatcggctgc acgatctggc ctccgctggc
      361 cgcgatggcg tcaagggttg cagccacact gtcaaccatc acgtaaataa ggaggccggg
      421 ctctccggca ggtgcgcgcc cgagcaccca cgagccgctt acttcacccg tgatgtcgtc
      481 aaatgcggta cgaccgtcag tgcgcgtcct gatgcgccag ccgaagacgc gccggtaaaa
      541 ttctgccgag ctcgcgatgt cggtggcggg aatctcgatg tagcagattt tcccatttcg
      601 cgcggcaggc ataagcgctc ccctacgcta gcacgctccg gggagtacgg caatcaggtc
      661 gagcgcccgc attgggggcc attgccgcgc aaatgttgga cggggatcgc gtgtccccag
      721 gcgatgtcgc cctgtgaaag gcccagggcg gcagccagcc tttggcgctg cgctgggctg
      781 ggcgtgtagt tgccgcttac gatggccttg accagcttcg catccagccc ggcggccgca
      841 acgagttgtt ccaagctcat tccggtttct tccatgtagc aggcgatcgg cttcataatg
      901 accgcatctt accacttcgc cggacgccca gcgcgacctt tagcagcgag ccgcggccgc
      961 cccgtcgctg gcgaggcccc gccggcaggc tcccgcgcta tcctggaaag gaacaaaagc
     1021 ttctgtcaaa ccgccgcccg cggcggccca ccttggcatg atcgctgaac cggcagggtg
     1081 ttggctttcc cgctttcttt ttgaaagatt gctcgccctg atctatctga tcgcgtttct
     1141 ctgcgcggcg aaccagtttg tcccgctgct cggaaaggac ggattattgc cggcgcccta
     1201 tttcgtccgg cggatgcggt ttcgcgcttc gccgagcttg ttctttttcg cgccgacgga
     1261 tcgcgctttc cgcatcgccg cctgggctgg cgtcgctctc tcgtgctttg ccgccgccgg
     1321 ttattcggaa aaatatggcg tcctggtttc agcggctgtc tgggcgtcgc tctgggtgtt
     1381 atatctttcg ttcgtcaacg tcggccagac gttttacgct ttcggttggg aatccatcct
     1441 gctcgagacc ggctttttca ccatttttgc gggcggcggc gccaccgccc cgagcgccgt
     1501 gctttgttgg atatggcgct gggtgctgtt ccgcgttatg ttcggggcgg gactcatcaa
     1561 gctgcgcgga gattcctgct ggcgcgagct cacctgcttg gattactatt tcgaaacgca
     1621 gccgattcca aatccactga gctggcactt ccactggctg ccgtcctggg ttcaccgcgc
     1681 aggagttctc ttcaatcatt tcgtcgagct cgtagttccc ttcgcctatt tcgctccgca
     1741 accagtcagc gccgcagccg gcctcatcac gatcgcattc caattcactc tcatcctgag
     1801 cggcaatctc tcctggctga attggctcac ggttaccctg gctgtttcca ctcttgacga
     1861 tcgatggctg tcgtggattc cggtgacgcc tccctcattg cacccggcgt acgcggcgca
     1921 tcgcgctgcg atctacgctt tggccgttat ggttgcggtt ctgagcgtcc ggcctgtcgc
     1981 caatatgctg tcgcgccatc aactgatgaa ttacgccttc aaccggttcc acctggtcaa
     2041 tacttacggc gctttcggcg gcattacgcg cacccgctat gagatcgtga tcgagggcgc
     2101 cgaagatccc gatcccggcc ccgcaaccca atggcgggaa tatgaattca agggcaagcc
     2161 gggagaccct gcgcgtcttc cgccgcagat cgccccgtat catctgcggc tggactggtt
     2221 gatgtggttc gcggcgatgg gctcagccta cgagcacccg tggctgatca atctcgcggc
     2281 aaagctgctc gagggcgacc ggcacattgt gaaactcctg aagaaa
//
LOCUS       DASREK010000238        54586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_940123, whole genome shotgun sequence.
ACCESSION   DASREK010000238 DASREK010000000
VERSION     DASREK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 54586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54586
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940123"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..662
                     /locus_tag="VFA54_12480"
     CDS             138..662
                     /locus_tag="VFA54_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51674.1"
                     /translation="MNLQFRIKVVEQTGEHGKLLAEADRIMPIPSGGPTERIGILAVK
                     LEDLGNQAWTLDLTDTSIPTLIVNREIGSKEYVRSNEIFFALLFPAVVREILTQVLII
                     DTEQKFEPDAESGDWQERWLYFVSSMARVDPVPAADADHMTKVQWIDQAIRVFCDSHQ
                     ACRTFLDSCKGETA"
     gene            659..991
                     /locus_tag="VFA54_12485"
     CDS             659..991
                     /locus_tag="VFA54_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51675.1"
                     /translation="MTARVLTDTGIQNFRAYLVRLRGGDTSAPPRELLDAPGSSAEFH
                     VPVEIENRSFASRLDAARYLVGKLGGKGEVDYNSGLWSWLALYYFDQIFPPLSGRSER
                     KPGTSRGR"
     gene            complement(1035..2756)
                     /gene="cmr6"
                     /locus_tag="VFA54_12490"
     CDS             complement(1035..2756)
                     /gene="cmr6"
                     /locus_tag="VFA54_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HZS51676.1"
                     /translation="MSRLPPDSQSHPGLLLDKWHDPWPVPPQPLPRGASKEQKERHRT
                     AKREFDEFARSQLERVTRASRDQALLDGVRQRFERAVATLPGRRWKRRTSESLTLHLS
                     RAGSFENSGICLHPIYGFAYLPGTGLKGMARSYARNVARAPDADIEAVFGKDTTKEES
                     GAAGGVIFFDALPVQWPKLTIDIVNNHHRQYYEGGGAPEDWEDPVPVNFLSVAPDTEF
                     EFSVASRHRSDDSGRLLELAQAWIDGALAWLGAGAKTNAGYGRFSTGAALDAGSNRRV
                     FTATLTFVSPAFLAGALQGKDDCALRPATLRGLLRSWWRTLHAGFLPLAKLRDLEGEL
                     WGTTVTDGAISLHVEPASAAKLKQFDFNKPRGADAVPGRFYLAYGMEGNPRQQKPARW
                     YVEPGAQWKLTIIARPAATWSGETVLDQAVMALWLLSEYGGIGAKCRRGFGSFELSEP
                     SLPLPTSKEVLARAASVRSGYQYNASMVESPPLNYMVFGVLPLKSRDVWSAIDQLGAA
                     YRSFTSSEKRKSEKLALGLPRNIGRRGFDQRAIEHPAGRSSCSIKSPSLSTGPLRSSF
                     AHSGRSW"
     gene            complement(2756..3121)
                     /gene="cmr5"
                     /locus_tag="VFA54_12495"
     CDS             complement(2756..3121)
                     /gene="cmr5"
                     /locus_tag="VFA54_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HZS51677.1"
                     /translation="MALKTLAQQRAQHAWDVVQQALKRPDFEKRFADPAKKMPARVRS
                     SGLGQTVAFMRAKGGGDVLKAVAGWCHQSGLIQESGEEGLLVQFKDGTAADLRQLTAE
                     ALAYLEWLVRFADAARKDK"
     gene            complement(3123..3953)
                     /gene="cmr4"
                     /locus_tag="VFA54_12500"
     CDS             complement(3123..3953)
                     /gene="cmr4"
                     /locus_tag="VFA54_12500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HZS51678.1"
                     /translation="MPANNVGGILYLQALTPLHPGSGTALGAVDLPVQRERHTDWPVI
                     PGSSVKGVLREATENGLRQRLFGAGDAEGTSAGALSFTDARILAFPVRSLKGVFAWVT
                     CPQALQRWSRDAGLVGKVVTLPKMSIDESTAHCAQGSPCVHNGKLILEEFVFDGKPFG
                     LDFFDALEAEERKRLVIMDDTNFSHFAKYGTEVTARIRLEEQSKTAAAGALFYQEFVP
                     AEALFYSLVLAAPSRRPGESVSPQENLAAFQPPGTIQIGGDETTGKGLCSLRLVKGAF
                     "
     gene            4046..4213
                     /locus_tag="VFA54_12505"
     CDS             4046..4213
                     /locus_tag="VFA54_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51679.1"
                     /translation="MKYTDPASTGQTPLVYAKAISARFDHQRGLALGIAMAGVGLGAA
                     LVPQFAHALIG"
     gene            4287..4976
                     /locus_tag="VFA54_12510"
     CDS             4287..4976
                     /locus_tag="VFA54_12510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51680.1"
                     /translation="MGGRVGEREAVVEKVPNAPLRGLTGFEALRSVRFWSLAVSFFIM
                     AGTTGGVISHLVPLLGDRGISAQTATGMVALAGIALIAGRLLAGFLLDRIHAPYVAVL
                     FFLAPLVGIAVLLSTPRPEGAAVGTILLGMGVGSEVDLIAFLVSRYFGMRSFGEIYGY
                     FFSIFMVGAGLGPFAMGVSYDRTRSYKLMLLCFMVALPLASLPMLRLGTYVYPSTRKA
                     GREHVLTSAQY"
     gene            5389..7125
                     /locus_tag="VFA54_12515"
     CDS             5389..7125
                     /locus_tag="VFA54_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51681.1"
                     /translation="MPTIRIVSAALLVTALLVAQAPVIGNCNVFPADDVWNTPVDKLP
                     VDPNSAAYISTIGASLGLHADFGAGLYNGGPIGIPFITVPGMQTKYPASFLYADESDP
                     GPYAIPLNAPIEGGSQSTGDRHAISVDTDNCILYELYDAFPQASSWKADSGAIFDLKS
                     YALRPAGWTSADAAGLPIMSGLVRYDEILAGEIRHAIRFTVPQTRRAYIWPARHYASS
                     LTGTQYPPMGQRFRLKASFDVSGFSPTNQIILKALQKYGMILADNGSAWFLSGAPDDR
                     WNNSDLHALGAVLGSNFEAVDESSLMIDPNSGQARQSGVTVTVSPPAANILVSATKLF
                     SASVSGTSDSSIIWSVNGVDGGDSAVGWIDSTGLFTAPNAVPSPATVNVQARSVASPT
                     SIGSAAVTIVPPPAISSVSPSPLPLGSFTMTVNGSGFQNGAAVKLGGAALATAFVSPI
                     QLKASGSASSTGSGIPVTVMNPDGGVSNAVTVDVVNLPVTVTVSPSSASVRVRNTKQF
                     TATVQNSSNQSVTWKVNGINGGNATVGTISASGLYRAPNSVPSPATVTVSAQSVAAPS
                     ASGSASVTVTKH"
     gene            complement(7213..7656)
                     /gene="mscL"
                     /locus_tag="VFA54_12520"
     CDS             complement(7213..7656)
                     /gene="mscL"
                     /locus_tag="VFA54_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HZS51682.1"
                     /translation="MLKEFKEFAMKGSVLDLAIGIVIGAAFGKIVTSFVNDILMPPLG
                     LLLGKVDFSNMFINLSPQSFDSLAAAKAAGAATINYGLFINTVIEFLIVSFAIFLLVR
                     QVNRMKRAPAPAAAAPVSKDCPYCLSIIPLKASRCPNCTSELQAA"
     gene            complement(7819..8655)
                     /locus_tag="VFA54_12525"
     CDS             complement(7819..8655)
                     /locus_tag="VFA54_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51683.1"
                     /translation="MEQTTPKTSHWALAALVAVLFGGNAYLLWRVHHLQAEMSDWRAS
                     MITQISELRETESEQSVSSRQKLDALNEELRAARLAAARSAGQAKIEAQRHAEQLAQK
                     LAAEQALQKAQQERVASELSDVKQAAGANDVKIADVSKEVAATKTELESTVADLKRVT
                     GDMGVVSGRIATNSQELAALKRLGERDYYEFTLVKTKQPQKVGDIRILLKKADPKHNK
                     YTLDVFADDKRIEKKDKNTNEPVQFYVAGARQPCEIVVNQVKKDQLAGYLAVPKMQTA
                     RN"
     gene            9228..10451
                     /locus_tag="VFA54_12530"
     CDS             9228..10451
                     /locus_tag="VFA54_12530"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS51684.1"
                     /translation="MHPKFLTPAVLYILSSVFLFPGCGNAPKEVRSAGAPPAVPVSVA
                     TAVQQSVPVEIHAIGTVEASKVVQVKSQIAGELMSVHFVEGGDIKKGDLLFEIDPRPY
                     QEALRQAEAAVAKDRAQLAQAEANLGRDRAQLKFADADAARYEALLREGVVSRAQYDQ
                     ARTNADALKESVRADQAAIDTARAALQSDLAAVDRAKLDLSYCQIRSPITGRAGNLLV
                     HEGNLVQANGDKALVVINQITPIFVSFGVPEEHLSAIRRSSSVRQLPVKAAPQDNPEK
                     NVAGVLTVIDNAVDSATGTIRLKATFENAERLLWPGQFVNVALTLDTRNNAVVVPSEA
                     VQAGQQGQFIYVAKPDQTVELRKVAVGISVGKGVVIDKGIAPGEAVVTDGQLRLFPGA
                     HIQSVPASNVDSKGL"
     gene            10635..13730
                     /locus_tag="VFA54_12535"
     CDS             10635..13730
                     /locus_tag="VFA54_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS51685.1"
                     /translation="MNVSRLFIERPVMTALVTFAILLFGIVGYRALPVAALPSVDYPT
                     IQVFAGLPGANPETMASSVATPLERQFSTIAGIQSMSSVNTQGSTQISIQFVLDRNID
                     AAAQDVQAAISTAQGQLPANMPRPPSYQKVNPADQPVLYLSLTSATLPLYTVDEYAET
                     LLAQRISMASGVSQVQVYGAQKYAVRVLLNPDALVARGIGIDEVQSAIQKSNVNLPTG
                     RLWGEKQAFTVQSSGQLTSAAAYRPMIVAYRNGLPVRLADLGRVEDSVENDKTIAWLN
                     GVRGVILAIRRQPGTNTVEVVDNIKALLPIFRSEIPPAVDLSVAFDASESIRASINDV
                     KFTLVLTICLVVMVIFLFLRNLSATLIPGVAVPLSIIGTFAVMYLLGYSLNNLSLMAL
                     TLSVGFVVDDAIVMLENIVRYMEMGKPRLEAAVLASREIGFTILSMTLSLVAVFIPVL
                     FMGGMVGRLLHEFSVTITVAILVSGFLSLTLTPMLGSRFLRYRQEGRHGTMYRALEAG
                     FEALARGYDYTLQRVLRRQLATLTVAVALLAGTLYLFFTMPTGFIPSQDSGFIFGVTM
                     AGQDISFESMAKHQKAVADIVQSDPNVRNTGAFAPPGNQGFVFMNLKPRSERRLNVDQ
                     VMDELRPKLAQVPGMLTFLQNPPPITISGQFTTSVYSMTLQSTNLQEIYTWVPRLLAK
                     MRALPGFLDVNSDLQIASPQVMVDIDRDRALALGVTPQQIQDALYTAYGDRQISTIYT
                     PANQYEVITQVEPQYQRTPDALSKLYIRSSQGQLVPLDEVVNITRNVGPLSVNHFGQL
                     PAVTVSFNLKPGFSLGEAAQRVDEAVRELRMPATLSVSFQGTVKEFQKSFQGLSILLI
                     VAILVIYIVLGILYESFIHPITILSGLPSAVFGALLTLKLFHKELDLYAFVGLIMLFG
                     VVKKNAIMMIDFALEAQRTENSGARDAIYRGCLLRFRPIMMTTVAALFGTLPIALAYG
                     EGADARQPLGLAVVGGLLVSQLLTLYITPVIYLYMERWQAWLRGEGAVREPREPAVAK
                     A"
     gene            13859..14392
                     /locus_tag="VFA54_12540"
     CDS             13859..14392
                     /locus_tag="VFA54_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS51686.1"
                     /translation="MSDRPANIHIAWATEREAPVILDLIRALAEYERLTHTVTATEEQ
                     LRRTLFGERPCAEALLASMEGEHVGFAVFFTNYSTFLARPGIYLEDLFVRPHARGKGI
                     GAALLASVARIAVERGCARMEWSVLNWNESSIEFYKKLGAAPMNEWTIYRLAAPGIED
                     LASRFPASFSLLRSSRR"
     gene            complement(14521..16938)
                     /gene="priA"
                     /locus_tag="VFA54_12545"
     CDS             complement(14521..16938)
                     /gene="priA"
                     /locus_tag="VFA54_12545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZS51687.1"
                     /translation="MPLYCDVSLPVPLDTPFTYLLPPALEASAQPGCRVIVPFGARKL
                     TGVILECHDRQPTAEARAVLRLVDEEPTLSRELLDLGRWIADYYRAPLGETLRVMLPP
                     AIDVRRVRLVSLTPAGQEAARQYSMFAEADDPVAEILRALERRPLAESYLKKKLPLAA
                     GVIRGLERKGLIRIDRQERLRDPLRAHNGRLIVESIPHRDFPRKVSKIEKRLLDFLAA
                     HPGPHEVEALEADVQGFEAAARRLAKAGILRLWKEPGEELAPAEPAPKPELSAGQRAA
                     LEAVAGSIRANKFQTFLLHGVTGSGKTEVYLHAIETALELGKSALLLVPEISLTPAAA
                     ALFHRRFGEQAAILHSAFGAAERAAQWARIRSGAARVVVGTRSAVFAPLQNLGLVVVD
                     EEHDASYKQEETPRYHGRDVAIVRGERAGATVVLGSATPCLESRYNAARGKYALIELQ
                     ERIEKRPLPEVDLIDMRLEFIETRQQRLFSRCLLDAIEARLAAGEQVMILLNRRGYSS
                     FVACRSCGERIECQNCSVTLTYHRREQRLLCHYCDYAERPPAACPKCGSEYLYFLGSG
                     SEKVEQTLHEAFPRARIARLDRDTARGGRRYESILGGFREGSFDILVGTQMIAKGHDI
                     PNVTLVGIVSADIGLGLPDFRAAERTFQLLTQVAGRAGRGELPGRVLIQTLNPDHYVI
                     QFAKAHDYQGFYEKELNFRRFLHYPPFAALANILVRSAKLEEALKLSGMLGEYLKNSP
                     KTVRVLGPAAAAMVKLKAEYRYQFLLKASSRRDLGQLLQGARRFAEEQKWPATALVID
                     VDPMSLM"
     gene            17132..18838
                     /locus_tag="VFA54_12550"
     CDS             17132..18838
                     /locus_tag="VFA54_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HZS51688.1"
                     /translation="MFRLDSHEWLIVVSVAALLVPVLLFPQAASRSVPTAKALKVTPD
                     LEQRLAKWQPVRMPFNAAGLSVRERQMIEKLVRACQYLDDIYWRQSDPQGLALYKSLA
                     ASRTPRNQQLRRMLMINGGRFDLLDENRPFAGTEPMPPGRGFYPEGLTREQIEKYVQQ
                     HPEKKAEIYSPYTVVRRRGRELAGVPYHQVYKAFLDPAAQTLREAAALSDDPAFARFL
                     QLRAAALLSDDYYESDLAWVDLEDPKFDLIFAPYETYLDDLLGVKGSYGAAVLIRDEE
                     QSRKLAEYQKYVPEIQDALPLPPQDRPSKRGHSTPMEVMDAPFRAGDLRHGYQAVADN
                     LPNDPRIHQAKGTKKIFFKNFMDARVNYIILPVARLLMRPDQAAKASAEGYLTFVMLH
                     EISHGLGPAYARKQGKQADIREAIGPIFSALEEAKADVVGLFGLHWMMRQGALPTQRS
                     EEYYASYVAGVLRSVRFGPGEAHGQAEMMEFNVLAQEKAVARDASTGRYVIDYRRMPG
                     ALERLAKELLEIEASGDRDRAENWFQRYGAMSPELKEALQAASGIPVDIDPIFSFAEP
                     VR"
     gene            complement(18869..19519)
                     /locus_tag="VFA54_12555"
     CDS             complement(18869..19519)
                     /locus_tag="VFA54_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZS51689.1"
                     /translation="MLIKAFFFDIDGTILDSATDICSSILAACSLAQKPEVSEGVLRR
                     YIGRSLAETFAEIYPGSSENEIERLCLLYREQYRERAHRGTRVFPGIQEMLAALPGLK
                     STATTKSSATTALVLELFGLRRHFDHVQGSDNGRYKPDPTILLEAAEALKVRPEQCLM
                     IGDAPVDMEAGRRAGMLTCAVGWGYGDRAELRSWHPDFWIDNPSDLVAIAQRLTLR"
     gene            complement(19594..21519)
                     /gene="ftsH"
                     /locus_tag="VFA54_12560"
     CDS             complement(19594..21519)
                     /gene="ftsH"
                     /locus_tag="VFA54_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZS51690.1"
                     /translation="MNSTIKTAIFWVVMICTAVLLWSVVKSGSPGREKTYTFTQFIDE
                     VQAGNVSEVTISNGNQVTGKLKNSSVTLRTTIPTNYPDIYKLLQEKKVNIDVKQADSN
                     NWVTLLVNASPFILLIAFWIFMMRQMQTGGNKALSFGKSRARLLSTQQKKVTFKDVAG
                     VEEAKEELQEIIEFLREPQKFQKLGGRIPKGVLLVGSPGTGKTLLARAIAGEANVPFF
                     SISGSDFVEMFVGVGASRVRDLFDQGKKSAPCIIFIDEIDAVGRHRGAGLGGGHDERE
                     QTLNQLLVEMDGFDSNEGVILIAATNRPDVLDPALLRPGRFDRRVVVPRPDVKGREAI
                     LKVHTKKVPLADDVDLTVISRGTPGFSGADLANLVNEAALHAARQNRKVVTMSDFELS
                     KDKVLMGVERRSMILSEEEKKTTAYHEAGHALVAAMMPHADPLHKVTIIPRGMALGLT
                     MQLPMDDKHTYSRQFLESQLAILMGGRVAEEIFLNQMTTGAGNDIEQATEMARRMVCE
                     WGMSEEMGPLTFGKKEEQIFLGREIAQHRDYSEDTAVHIDAQVKRLVTTGYQRAKQVI
                     QEHSDALVRIAEALLEREVLDGAEIKLLIEGKTLPSITPAPPRTPAEEEPTQKVLRPD
                     TGKRIPGLSEGERPQPA"
     gene            complement(21516..22964)
                     /gene="tilS"
                     /locus_tag="VFA54_12565"
     CDS             complement(21516..22964)
                     /gene="tilS"
                     /locus_tag="VFA54_12565"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZS51691.1"
                     /translation="MIQRVAQTIRKYSMLRAGERVGVAVSGGPDSVGLLRVLRELQSE
                     LGVLLAVVHLEHGLRGAESKADAEFVRDLARSLNLELALETARLADEISKTGENLEQA
                     ARRLRYGYFQRLLRENQFQKIAVGHTKSDQAETVLFRLLRGSATAGLAGIYPVFQGGI
                     VRPLLEVERGEVLDYLRSLGQNWREDSSNRDLSIARNRLRLDLLPRLEREWNPSIINT
                     LAQLADWARDEEAYWRNEIGLLAPQYMRLEGPMLYLDAPGIAALPAAVARRLLRHAVE
                     RVRGDLRGIEFAHIEQIRSLAALPEGDGRVRIPGVDVFRSFSQIRLAPVESVKGLEDR
                     NYSVPLSVPGTYEIPGGRTRLILKLGTDRDSDRGYNNTVGCLDWERTPRPLWLRNWRP
                     GDRYHPRGWSGEEKLKTLFQQHRIPVWERRHWPVVAAGSAIVWARRFGVAAELAAGPE
                     CRTVLEITESESGQEKSTESGAEASNRIGDVV"
     gene            complement(23394..24665)
                     /locus_tag="VFA54_12570"
     CDS             complement(23394..24665)
                     /locus_tag="VFA54_12570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS51692.1"
                     /translation="MQPNRRGSLLVLLLLVTGAGAYIALSAAPRAAAPGGSIDGVWDA
                     VVAVNDLEIPFRIEFSQAGKELRGAFLNGDVKEKSSSGEMEGDHIHLRFDYWNSDLRA
                     SLHGGKLEGSYDHKGRTGATSYPFRARRFVPATALETPAPSLAGEWILTSDGTIHPGR
                     PNVWRFLVRQSGAEISASILRLDGDTGTLSGRLQNGRLVLSHFSGARPMLLEAVLHPD
                     GSLEGTLNRRTKFTAVRKSEALARGLPLPPDPSRYTSVKDPSEPLRFQFPDLEGRTVS
                     NADSRFRGKVVIVNIMGSWCPNCQDEGPFLAELYRKYHDDGLEIVGLCFESGDLEIDR
                     SQVRAFMRRHGIRYPMLLAGVVEPGAVPKALPQLVNFDSYPTSIFLGRDGRVRSVHDG
                     FASPATGEEHARLKREIEELVQRLLAEKAVS"
     gene            complement(24874..25263)
                     /locus_tag="VFA54_12575"
     CDS             complement(24874..25263)
                     /locus_tag="VFA54_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51693.1"
                     /translation="MFRSLWLGTAASAAAVLLLFLCYLLFRNPATPEERERRRRAAVN
                     AAGRITDGSITEVEVFDTPGGATVHVLHFSYTLNGVTYSAAQDITSLLAYIDRDPLRL
                     AGPVGVKYLPGNPSNSIVICEAWSGIR"
     gene            complement(25267..25926)
                     /gene="fsa"
                     /locus_tag="VFA54_12580"
     CDS             complement(25267..25926)
                     /gene="fsa"
                     /locus_tag="VFA54_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HZS51694.1"
                     /translation="MKFFLDTANLKEIQAGVDLGLVDGVTTNPSLIAKEGRSNAEQIR
                     AICDLVDGDISAEVVATTAPEMIAEGRSLARIHPNVVVKCPLIREGIKATKALSSEGI
                     RVNVTLCFTATQALLAAKAGAYIVSPFVGRLDDISTNGMELIRQIVKIYQNYGYPTKV
                     LVASIRNPLHVLEGALAGAHIATMPFKVLDSLFNHPLTDKGLAAFLADYQKAFQETTV
                     G"
     gene            complement(26041..26841)
                     /locus_tag="VFA54_12585"
     CDS             complement(26041..26841)
                     /locus_tag="VFA54_12585"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZS51695.1"
                     /translation="MRFVRFAVDKQAPATIRDGAGGVRWGIVEADRYRQITGDPLDQW
                     IPSDRLYSPDEVRLLPPCSPSKIVCIGRNYAEHAREFSNPLPSEPLLFLKPPSALIAS
                     GDAILYPPQSERVDYEGELGIVIGRRCSQVADPAAALAHAFGYTCLNDVTARDLQKRD
                     GQFTRGKGFDTFCAVGPYLVPASEVDASQLPVRTYLDGELKQNGNTRDLIFPLGAIIA
                     FISQVMTLEPGDLIATGTPSGVGPMRPGSEVVVEIEGIGSLRNRVIRR"
     gene            complement(26873..27811)
                     /locus_tag="VFA54_12590"
     CDS             complement(26873..27811)
                     /locus_tag="VFA54_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HZS51696.1"
                     /translation="MPPLLNIPARTADLQASQRGAELILQWTAPGQTTEGIALKQVDR
                     TSLLAKVVETGSVDAASFDTGARELATWNDLAGGQKVERHLPLPASPGVRLALAVKNF
                     SARGRTLGVSNIAIVQIGPLVSPPPKLSATVQPAAIRLEWAPVSGAAGYRVYKRSGEN
                     PQFAYLGSTTAPLFEDSNFEWGEPYAYFVRAYAEVSTGIDESADSPVAAIVPHDVFPP
                     SPPAGLRAVATETSAEISWNLSPEPDTAGYNIYRRVLSGSAPSPPVRLNAELLSAPVF
                     SDKDVQRQRRYVYTVTAVDDKGNESGPSAPLEVAIP"
     gene            complement(28021..29385)
                     /gene="hslU"
                     /locus_tag="VFA54_12595"
     CDS             complement(28021..29385)
                     /gene="hslU"
                     /locus_tag="VFA54_12595"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZS51697.1"
                     /translation="MVIYLPSQSVDDTPALDELTPREIVRELDKHVVGQHEAKRAVAV
                     ALRNRIRRQKLQPEMAEEVMPKNILMIGPTGVGKTELARRLAKLANSPFLKVEASKFT
                     EVGYVGRDVDSMIRDLVEIAIDMVREEKLDLVAEKAEANAEDRILDLLIPPQPQADGA
                     DVQKSREKLRTQLREGKLDDRIVEIETRERSFPSFEIITNTGVEEMDINLKEMIPGLF
                     GQKSKKRKMRVAEALDYLVQEEEQKLIDMDQIQRIAVDRVEQSGIIFLDEIDKIAGRE
                     GGHGPDVSREGVQRDILPIVEGTTVNTRYGMVRTDHILFIAAGAFHVSKPSDLIPELQ
                     GRFPIRVELKPLTVDDFVRILQEPKNALVKQYTALLETEGIKLTIGNDAILEIANYAA
                     KVNENAENIGARRLHTIMEKLLEEISFEGPDLKKKNFRIDADYVRKQLADIVKDQDLS
                     RYIL"
     gene            complement(29508..30047)
                     /gene="hslV"
                     /locus_tag="VFA54_12600"
     CDS             complement(29508..30047)
                     /gene="hslV"
                     /locus_tag="VFA54_12600"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZS51698.1"
                     /translation="MGQRVRSTTILCVRRNGKVVMAGDGQVTVGSEVLKHSARKVRRL
                     YNDKVLAGFAGSTADAFSLFERFDAKLEQFQGKLSRAVVELAKDWRTDKVLRHLEALL
                     LVADAGYTFIVSGNGDVIEPDDGVAAVGSGGPFAVAAATALMKHTELDARKIAEEAME
                     IAGKICIYTNQNVTFEEIG"
     gene            30293..31339
                     /gene="aroF"
                     /locus_tag="VFA54_12605"
     CDS             30293..31339
                     /gene="aroF"
                     /locus_tag="VFA54_12605"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZS51699.1"
                     /translation="MLVVMDAHATQEDVARVCRKIEKLGFRPHVMPGAQRTAVGITGN
                     PGPIDPAEFEDLPGVAEAIRVSKPYKLVSREVKPDDSVIEIAGVPVGGSALTLIAGPC
                     SVESRGQILAVARAVAAAGAQLLRGGAYKPRTSPYAFQGLHEEGLQLLAEARAATGLG
                     IVTEAIDVETFDLVERYADCIQIGARNMQNFSLLRRAGRSRLPILLKRGISSTLEEFL
                     MAAEYILSEGNYQVVLCERGVRTFADHTRNTLDLSVVPAVQRLSHLPIIVDPSHGTGR
                     RDKVLPMARAAVAAGAAGLLVEVHTDPDHALSDGFQSLYPGQFSQLAEECRAIANLLA
                     SQKAKTAADARAAR"
     gene            31353..32483
                     /locus_tag="VFA54_12610"
     CDS             31353..32483
                     /locus_tag="VFA54_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HZS51700.1"
                     /translation="MIDAGRRGVVCALAALSLFAWSCRPRRGTAVEGYAFVATAAGNA
                     ITVVDLASFAAIRQIPLGSRPMQIVSDPARRSLYVMGEGGPAGLTEIDTARLVAKRSL
                     WLAEKPQRIRLAPGGGRLYLLDARAQTFQSLDLEKMTRGRQIRLAGPPVDFDVSPDGL
                     WACVSLQWGEVQVLDLARWKASAEIQTGGSPAAVAVRSDSRQAFVANPDSRSIAAIDL
                     TAGRVVAHLPLSARPEVMRFKPDGGELFVSGGDTSAVVIVSAYRDEIDQPMLAGAEPR
                     GLAVTKDNRLLFVANSAANTVSVINIDDRRMLASVPVGEEPRQVALTPDDQYALVLNY
                     KSGDMAVIRVAAVVGGSRDRIQPLFTMIRVGSQPVDLAVQTR"
     gene            32864..32939
                     /locus_tag="VFA54_12615"
     tRNA            32864..32939
                     /locus_tag="VFA54_12615"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32897..32899,aa:Ala,seq:ggc)
     gene            33287..33766
                     /locus_tag="VFA54_12620"
     CDS             33287..33766
                     /locus_tag="VFA54_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS51701.1"
                     /translation="MRAIAISFAFTLGAAILAAQSQSVKTFSSAKDVEALIAKAKSER
                     KDNQPVVTERILQLPPYNANLEYRAAVGPASIHEREAEIFYVIDGSATMVTGGKLVDE
                     SRTNPNNLTGKGIEGGSSREIGKGDFIVVPEKTPHWFSAIHGTLVLMSLHVPRPAAH"
     gene            complement(33979..35628)
                     /locus_tag="VFA54_12625"
     CDS             complement(33979..35628)
                     /locus_tag="VFA54_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51702.1"
                     /translation="MTFSRQAAGARRAATTFAVLFQIRKCVTQATAVLLCLAAVNAPL
                     SGGALSSTARAIREEATRANNAAAGRPLPLAAHWNTHAFSPNYQLEKIESGGYVLLSF
                     ALPGPDAALTPAALAYYKAAIKRAAELKLPISLVGTNFASILYEPPYYLLPASQNPNV
                     IQRDGAIRKQLDPMGPVEPWIDAGRRLVSSSLLQQIERWYPDPPLVVFVSNNEESRIA
                     WTAIEQSKRFLDAYGPAHDGDFKRSVVAQGWIERYRALLQAMRGGLIAPAWRSHSIFI
                     GYDAFGPPHYARWEQWDDYALPTGRFIDPAPLIWDGGSPSFYLHNWNASTDYNIWSPQ
                     GEALNWVFMLREAMRLNPRFWFGFSTWDGEQEKRDQLAKVAPFTPARYAAMVQFGLWL
                     LRPRCVREFRGWTDSAEKAGPWLDAILEAVGSIHRNGALRRFWRRGELAPNRAWKHPY
                     QSRPVERYAAEDRWFLLDTTLDPPRPYNAATPLPVFSLSLVLGRAPRREWLLYAHAPM
                     GSRRNVGITIPGYRQVVVDVSAGGSYYLIRENRPSPERIEP"
     gene            complement(35625..36659)
                     /locus_tag="VFA54_12630"
     CDS             complement(35625..36659)
                     /locus_tag="VFA54_12630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS51703.1"
                     /translation="MKFVLLLLAAAAFAADQPSVDRAVQLATAGKLNEAEAMLTALEK
                     AHPRDADLQYRLGLVLLKHGKLDEARRHLDLAAKLNPKSPLIWRALALLHNTAGKAAA
                     AHNDAATAVKEFQEAIRRDPERAPYYLDLAQLFLDRDTPEPAEIVLRNASQRFPANVN
                     VLRSFGLALYALGKYDEALVVFLKAIDADPDLEEAYASLEVLLPYAQPRLPEVVSRLR
                     RFCERKPSSPIGPYLLGLVVPEESEALFRKAIEAAPGFWPAYFELHKLLKGQGKWDEA
                     AAALQKTIDLNGSYAPAHYALAQYYDRKGDRDRAAQEREIHHKLLNDQRVAAEKQRER
                     APRLAYTLEQ"
     gene            36884..40663
                     /locus_tag="VFA54_12635"
     CDS             36884..40663
                     /locus_tag="VFA54_12635"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS51704.1"
                     /translation="MVNRRQFLTSATLLPAALGAQQEGPPGPAVEDPVEKITPGEKPT
                     VAVCHVGFRPRVGRKTLVVRAAAGAPAKELMTGVGARNPMPFLPGGASMLRHGIVIAC
                     LLACATLLQGQTSTGTIQGQVRDASGAVVPDARVTITNLRTGVLNELRTNGEGRYVMP
                     FLLPGDYAIAVEKPGFQRFSRSGIKLDVGQNESVDVTLAVGDVATTVQVEGTPPPLAT
                     NSSTLATVIENKRIADLPLNGRNPFDLAALSPGVIPMAPGGLGAGMSPTPSISGGRDA
                     NSEISIDGTSIVLPENNVSITTMAFTPSVDAIQEFSVMTNTLSAEYGRSGGGVINVAT
                     KQGANQLHGSAFEFFRNSELDANDFFSNRAGIPRHAFQRNQFGGTIGGPVEVPGVYRG
                     RNRTFFFFDEQSTRQRSFSQFTNTMPLDAWRNGDFSGLRNSAGAPLLIYDPLTTRPDG
                     QGGYTRDPFPGNQIPSARIDPVVRKTEAYFPEPNTTPINPFTQVNNYIMVGKSPTNDD
                     RFDVRLDHNISDRWRIFGRFSHQFNKVTPVNFFNNVATPSGNGPESFSFYSASLDQTY
                     TLNPTTILNLRYGFGRFFDVRRPFSAGFDMTTLGFPQAVRDQAATELLAFPRFDINGI
                     SSIGASGFTNVRIVPNSHILHGDLTRVLSRHTLKLGVEYRKLLENFLQDAAPDGTYSF
                     NQQWTQRDPVTPSSTAGFGFASFLLGIPSSGSISHEPATATASSYWAAYIQDDFHATS
                     NLTLNLGLRYEVDVPRTERYNRLSYFNLNEPSPIAGRVPGFPNLVGAMHFVTPGHRQQ
                     TPTDWNNFGPRFGFAYKIGSKTVARGGYGIMYAPSVMQAAGYTGTMGTEGITSSTNFI
                     TSLDGRIPINFVSNPFPNGFNLPLGATPGPTSGPSTDLGLGAGSSWFIDYRNALIQQW
                     NFNVQRDLFSNTVIELGYVASKGNHLVDGEGGMAYNQLPSSFFSLGNSLNNLVPNPFY
                     GIIVNPTSVLSQPTVTLAQLLRPYPQYTSLSPSRKPQANSLYHSFTLRAEKRYSQGLG
                     FLLSYTFGKLIDDASSTVTFLGPSGNKQDFYNRKAERAVSTQDVSSRLVISTVYDLPL
                     GRGRKFMNNAGEPWQFIFGGWQTNGILTFQTGTPVILTQTQNNTALGSPGQRPNNNGQ
                     SAHVSGGSKDSRLKQWFNTSVFSFAPPFTFGNVGRTLPDVRNPGQRNADLSLFKNFRF
                     REDRFTLQFRAEAFNAINTAQFGPPGAQVGSSNLGVISSSGVSARQMQLALKLLF"
     gene            complement(40757..41692)
                     /locus_tag="VFA54_12640"
     CDS             complement(40757..41692)
                     /locus_tag="VFA54_12640"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HZS51705.1"
                     /translation="MKILLFALATVLLSLAPAPAAELKQGPPLPYHLVRDWAKLPKGM
                     NFGEASGVDVDRNGNVWVFNRSDHPIVELDKHGEVIQAWNENVLRVKGAHGARVDPDG
                     NLWFIDRDGHVVFKTTPAGKILMLLGRQGIKGDNDSKEYFNLPTNVAFAPNGDFFISD
                     GYGNSRVIKFNKDGEYLLHWGKKGVGDGEFNLVHDVALDSRGRVYVADRSNSRVQIFD
                     QNGRFLGKWTDVGQPWGLYYVPKEDALYMCDGLNNRIVKLNTEGQILGTLGSYGKIPG
                     KLDYAHSIAVDQNDGSIYVSEIKNWRVQKWAKNAN"
     gene            complement(41905..42807)
                     /locus_tag="VFA54_12645"
     CDS             complement(41905..42807)
                     /locus_tag="VFA54_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51706.1"
                     /translation="MRRLIILPTIVICLALASCKTGPPPSERLHATVLMRDGTQVSGI
                     VTASSPSQITLAGDDNTTRTIAMKDVQSVNYDQQPVAQAEAPPAAPSQSAPPARQPQP
                     PVQSQQPVHQGHYHPQQSAVQTKTYALPVGTQVSVRTEETIDSSKAVEGQTFAAEVTS
                     DVLDANGDVVIPRGSNAQIVIRSASKGGRFRGASDLALDLQTVSVDGRQYRLDTADVQ
                     QRGKEGIGANKRTAEYTGGGAALGAIIGAIAGGGRGAAIGAASGAGAGALTQILTKGS
                     AVRVPAETVLTFRLDKPLRVVAAR"
     gene            42958..43131
                     /locus_tag="VFA54_12650"
     CDS             42958..43131
                     /locus_tag="VFA54_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51707.1"
                     /translation="MWKTIAQALRLGVPEQQLDAIGPILDSLWNDTRRVLDRDLSLTD
                     PAITFQAGLGGEL"
     gene            43128..44540
                     /locus_tag="VFA54_12655"
     CDS             43128..44540
                     /locus_tag="VFA54_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZS51708.1"
                     /translation="MTIAELGAQIQSGKISPAELAAETFTQIRKLNPVLNAYITVMES
                     SAMDQARQAEREIRAGNYRGPLHGIPYAAKDLFYTKGTATTVGSKIFQNFTPDYDAAV
                     IERLRDAGAILLGKAGLHELAYGITSNNPHFGTIHNPWDLSRIPGGSSGGSTAALAAG
                     MCAFSLGSDTGGSVRIPAAFCGVVGLKPTFGCISRHGVFPLGHTLDHVGHFAWTVRDA
                     AIVLNAIAGHDPRDGSSAVRAFRPISLPADVKLRGLPIGIPRNYYFERLQPEVENAVR
                     AAVKTVEDLGAEIREIEIPEIEDFTVIGRLILMPEATAVHRRLLQTRRADIGADVLAL
                     LDEGQFILAADYLDAQRRRRQLIQTFQRIFEQVRAIVTPATPLTAPKIGQSTIEIGGC
                     QEDVRLGSTRLVRALNVAGVPALSVPCGFDAQGLPIGLQIFGRAFDEANVLLVGHAYE
                     QATEWHKRRPEIAVAPAVVR"
     gene            44741..46504
                     /locus_tag="VFA54_12660"
     CDS             44741..46504
                     /locus_tag="VFA54_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HZS51709.1"
                     /translation="MAEPLQTSGVMAASPRGQAFPKAASARIGVAISAQRRPPLLVLG
                     AFLAVWSVWSFRIHASAAFSQYHDDTMLMVSAQALAENGHYTIPSLAVPTSASPPQTK
                     YPIFVPWLFSLVWRWNPHFPANLEAMQALAAFFGVLALAAAYAVLRTLKVGAWAAVGM
                     TIFLALQPVFVVLSTKLLTDVPMMALTLAAAAAAERALGKRQGFFLCAAAGFLAALAV
                     LTRTIGVAIVGGFLIAAVLRRRFRMAAVFIACCAPVVVWGLSRAHDSHVLDPWVRSGG
                     AAFQSNWLFYTNYIGFWKLSVSSLHAFGYLIAQNFLMFAITPGLYFQFPPPGGLLIYL
                     LFPLTAAILAGVLRHARQNGWQVIHFVFLLHTAIILCWTNREMIERFLLPFLPLLCAG
                     LWTEGRYLSALFVQSFRAGRARARFLMAPLAAGVVLFAARGLEAGARYDWDYVTEVIP
                     SHARRLQQKRELYAWVNANTAPSATFAAWDDGGLYLYTRRRGMRPIAFSTEYYYSRNP
                     QTLTAEMAHIFDTARHIHAQYWVRVDGEYADTQIEKEIEKVLAPLPVLFRSSSGAVRL
                     YDLRPVVTSPASQEEASAPRL"
     gene            46555..48207
                     /locus_tag="VFA54_12665"
     CDS             46555..48207
                     /locus_tag="VFA54_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51710.1"
                     /translation="MGAILAAFLGYTRSLHPENNFGRYHDDTVYFSAAQALAAGQGYT
                     LPSLPVPGGTVAPQSKYPSLYPWLLSWVWRWKPLFPQNVTLALRLTVFFASWFLIASF
                     YWLRSWLGECAALICVALTALHPHFLALSGAVLSEVPFMALAMTAIAWNDLAPENERT
                     WAPLGTGVLAGAATGIRSMGAAVVLGLAAAAVSRKRYRDAVLICLAAAPVVAATLPAG
                     AVALPGGPAASAGWRQTWLYYTSYWAFWKLSVPTWPVFLEMVQSNVKDLLVTPASQCL
                     FPAPAALGPFGGALLCILLTAGITAGIIRKARRQGWQSIHWVLLFYLPVTLVWNYPIM
                     NRFLVPFLPLFYAGAWMEGAHLAGMVRKTFGQRGALTEKMIAGLIAAGLAGTASAAAA
                     TYMNDSRARLREDGKQRAALQTQKAEAYHWITVHTDASAKILASEDICLYFYTGRQAV
                     WPIAFTTAPFYETDTRDLQLQLNHWTDVGRTVGARYWLAADGDYYIEAVSPLVRDKMA
                     ASQAALPEVFRSSAGAVRIYDLSSWQTEFSGQRISWSRPAVK"
     gene            48738..49403
                     /locus_tag="VFA54_12670"
     CDS             48738..49403
                     /locus_tag="VFA54_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS51711.1"
                     /translation="MITIATCESQPIVVEGLRRSLEGTKDLQFVGSVATAKAAIELVE
                     QQKPSVCLIDKAFGTKAVFQLIADMKVRSPQTEPILWAAEISEIESFRALQVGARGIL
                     KKTLPVATILDCLRTVARGNIWIENSISDQFVGFINRRNTPHLTAREEEIVALVTRGM
                     KNKQIADALCITTGTVKVHLMHIFEKTGVKDRFELAMYGRRMDAGSLELEENQALAAG
                     FGG"
     gene            complement(49416..50936)
                     /locus_tag="VFA54_12675"
     CDS             complement(49416..50936)
                     /locus_tag="VFA54_12675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS51712.1"
                     /translation="MTSTQAQAASGSRRAMERYVRRTLAVLSTGALALIAAHIGAALW
                     AQHEVTQNESAVAIHSLGLASGQGLYHKLGSYPYTVSPYMPLFYAAQAALHRLGLPPF
                     QAGRLLSILGLTGILWGVWKILRLCTGETHATWAGTLLAASTANLMFWGTVGQVDVLG
                     LCFSITALYFFLRYRRAQETRFLLLPGAFVLAALFTKQTLLAAGFTIFVCLAFENRRR
                     AVRFAVCIAAVGISLAAGVNYGTSGGFLENTVFANINPFSFVKFAAQLRYLAPVACGS
                     MLIALVGLPASIREGLHPLYIYAGASMAVLLSTGSKIGADLNYQLETMTALALCAGWS
                     LHRLRFFPLCFEKSRSSVTLLQAPLLVYIALNLVMSGRILSDRISQEMRRRQEYAQLR
                     PYLDSAKGPVISVQFDPLVQSGRRLEVEPLIYTLMVGAGRVDPGPVCRDLAAGKFSLV
                     ILFEDLLHPAAVPRNAELPSLPAPALDAIRRNYRLVEHIPGSLLAGDYIYEPAAAN"
     gene            51090..52784
                     /locus_tag="VFA54_12680"
     CDS             51090..52784
                     /locus_tag="VFA54_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51713.1"
                     /translation="MRQTGRSRAIVALILLAFLAELGARRPQDWFGWYHDDTIYFSAA
                     RSLAEGRGYRLPSVPGTPLETKYPVLYPWLLSWIWRWRPSFPANVSYAIGLTALFACW
                     FLIASFQLLRSLGFGNAGAACIVALCAFQPSFLFLSGAVLSDVPFMALAITAAVMADS
                     AMRPGTRMTRAAAAAAVTGLAGGMRGFGMALAAGIAIFAIYRRAFRQGAVFCAIAAPL
                     LVLSLSWPHANSSLEAAAAGDPGWRQTWLFYTSYGKFWKLCVPTAGVLFAMLGENVHA
                     FLQAPASLCLFPPLGEGSYAGTLLCVTLSLGILAGAVRQARRSEWKPIHFIFVFYSCL
                     ALIWNYTLMDRFLLIFAPLFYSGLWAETEHLVSMLRDNFQPRRPFSEKALAGVLGLAL
                     AAVGARAAVEYLHHFQPDSSELKAKRAALAREKSETYEWIRKNTGPAERFVAYQDVSL
                     YLNTNRQALRPIVFSTQAFYKKDDQVLRRDLAHITDVARHIGARYWLASDDDFQLETG
                     LPLIEKRMAEIKAVLPVVFHSRTSKVQVLDLSCLLHAEQRACQVIVPVLCPTTTGS"
     gene            53139..>54586
                     /locus_tag="VFA54_12685"
     CDS             53139..>54586
                     /locus_tag="VFA54_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51714.1"
                     /translation="MSETRPGQNSRWIPSLSEMVFVSVMAWLFLGASGPEALLTDGDT
                     GWHIRTGDYILATHSFPKQDLFSFSKPDQPWFAWEWLSDVIFAGLHKFGGLAGVVLLA
                     GVLIAATSAVMLRYMVWQRSDLFVSIVVMLVASSASTVHWLARPHLFSYFFLAIAIWL
                     LEADRQRPTRWVFALVGLCLAWTNLHGGFLALLSTLAIYLLGSAVETVWNKSGPQGWA
                     QTKRYGLLFGLASAATLVNPYTYHLHRHIVEFLRSDFILQHVAEFQSPNFRGEGMRYF
                     EVMLFAGLAAVPALLRRKDVTQAFLLLFWAHAALLSGRHVLLYVVVAAPVVAREATQL
                     WERAAKAGIGWLATLKQVADDYAGGCFHPAGGTGRRRAIAWMAPVCVVAMGATLFAGQ
                     RSERLRSEFPKTLFPVDACTAAESQFAGRRIFTSDQWADYLIYRYYPKIKVFIDGRSD
                     FYGPQLGNEYIRAMNSDHAWAQIFDRYRFDLA"
BASE COUNT        10305 a        16691 c        16403 g        11187 t
ORIGIN      
        1 atccatcaga agtccacgac cgcgccgccg acccgagcgc tggcgccagg ggttgaggct
       61 ctcgacctgc gggcattcgt cagtgcgctg ggcggcaaaa tggtccggca gcgaaagact
      121 cgaaaggcgc cggtcgcatg aacctccagt ttcggataaa agtcgtcgag cagacaggag
      181 agcacgggaa gttactcgct gaagcggacc ggattatgcc gatcccatcc ggcggtccaa
      241 ccgagcgcat cgggattctc gccgtcaagc tcgaggatct cggtaatcag gcgtggactc
      301 ttgatctgac cgacacgagc atcccaacgc tgatcgtgaa ccgggaaatc ggcagcaagg
      361 aatacgttcg ttccaatgaa atcttcttcg cattgttgtt ccctgcggtc gtgcgggaga
      421 ttctgaccca ggtcctgatc atcgacaccg agcagaagtt cgaaccagac gccgaaagcg
      481 gagactggca ggagcgctgg ttgtattttg tttccagcat ggcccgcgtg gacccggttc
      541 cagcggcgga tgccgaccac atgacaaagg tccagtggat cgaccaagcc atacgcgtat
      601 tctgcgatag ccatcaagcc tgccgcacat tcctcgactc ctgtaagggg gaaacagcat
      661 gactgcgagg gtgttgactg atactggcat ccagaacttc cgcgcgtatc tcgttcgttt
      721 gaggggaggc gacacctcgg cgccaccgcg cgaacttctt gacgcgccag gcagctcagc
      781 cgaatttcac gtgccggtcg agatcgagaa ccggagtttc gctagtcggc tcgatgcggc
      841 ccgatatctt gttggaaagc tcggcggcaa gggagaggtt gactacaact cgggtctctg
      901 gtcgtggctt gccctgtact acttcgacca gatcttccca cctttgagcg gcaggtcgga
      961 gaggaagcca ggtacctccc ggggacgata agctggcggt attaccggca tcttctagcg
     1021 gaaccgttcc gagtttacca acttcgccct gaatgcgcaa agctgctcct tagcggcccc
     1081 gtcgacaagc taggcgactt tatggagcag gagctcctgc cggcgggatg ttcaatcgct
     1141 ctctggtcaa agccgcggcg gccgatgttc cgaggcaacc ccaaggcgag cttctctgac
     1201 ttgcgctttt cgctcgacgt gaacgaccgg tacgccgctc caagctggtc gatagccgac
     1261 cacacatcac gggacttcag cgggagcacg ccgaaaacca tatagttcag cggtggggat
     1321 tccaccatgg acgcgttgta ctgatagcca gaacgaacgc tggcggctcg tgccagcact
     1381 tcttttgagg ttggtaacgg cagactaggt tcgctcaact cgaaagagcc aaacccgcgc
     1441 ctacacttcg cgccgattcc accgtactcc gagagcagcc acagcgccat aaccgcttga
     1501 tccaggaccg tttcgccgct ccacgtagcg gcaggccggg cgatgatggt cagcttccat
     1561 tgcgcgcccg gctcgacata ccacctcgcg ggcttttgct gtctgggatt gccttccatg
     1621 ccgtaggcaa gatagaagcg acccggaacc gcgtcagcgc cccttggttt gttgaagtcg
     1681 aattgcttga gcttcgctgc tgacgctggc tcaacatgca agctgatcgc gccatccgta
     1741 accgtcgtgc cccatagttc accttccaga tctcgcagct tggcgagcgg cagaaagccc
     1801 gcgtgaagcg tccgccacca agagcgcagc aaacctcgga gcgtcgcagg ccgcagggca
     1861 cagtcgtctt tgccctgaag agcgccagcc agaaatgccg ggctgacgaa cgtcaacgtg
     1921 gcggtgaaaa cgcgcctgtt acttcccgca tcgagagcgg caccggttga gaatcgtccg
     1981 taaccggcat ttgtctttgc tcccgcgccg agccaggcca gggcgccgtc gatccacgct
     2041 tgggccaatt ccagcaatcg gccgctgtcg tccgatctgt ggcgtgaggc aacgctgaac
     2101 tcgaactccg tgtcaggcgc taccgaaaga aagttgaccg gaaccggatc ttcccagtcc
     2161 tcaggcgcac caccaccctc ataatattgc cggtggtgat tgttgacgat gtcgatcgta
     2221 agcttcggcc attggaccgg aagagcatcg aagaagatga ctccgcccgc agcgccgctt
     2281 tcctccttcg ttgtgtcctt accaaacacg gcctcaatgt cggcatccgg cgcgcgtgcc
     2341 acattgcgcg cataggatcg cgccattccc ttgaggccgg tccccggcag gtacgcaaat
     2401 ccgtagatcg ggtgaaggca aatgcccgag ttttcaaacg atcctgcccg cgaaagatgg
     2461 agcgttagcg attctgaagt ccggcgtttc catcgccggc ccggcagcgt tgccacggcg
     2521 cgctcaaagc gctgccgcac cccatccagt agcgcttgat cgcgagacgc gcgcgtgacg
     2581 cgctccagtt ggctgcgcgc aaattcgtca aactcacgtt tggctgttct gtgtcgctcc
     2641 ttctgttctt tcgatgcacc ccgaggtagc ggttggggcg ggacgggcca gggatcgtgc
     2701 catttatcca gcaacagccc aggatgcgac tgggaatcgg gaggaaggcg agacattact
     2761 tgtcctttct cgccgcatcc gcgaaacgga caagccactc aaggtacgcc agagcttccg
     2821 cggtcaattg acgcagatcg gcggccgtgc cgtccttgaa ctgaaccagc aggccttcct
     2881 cgccggactc ctgaatcaac ccagattggt gacaccagcc cgcaacggct ttcagcacgt
     2941 cgccgccccc tttggcgcgc atgaatgcga cggtttgacc caggcccgaa gaccggaccc
     3001 gagccggcat cttcttggcc gggtcagcga atcttttctc gaagtcgggc cgtttcaatg
     3061 cctgttggac gacatcccag gcgtgctgcg cacgctgttg tgccagggtc tttaacgcca
     3121 tctcagaacg ctcctttcac caggcggagc gaacagagtc ctttgccggt ggtctcgtcg
     3181 ccaccaatct gaatcgtgcc tggcggctgg aaggcggcga gattctcttg gggcgagact
     3241 gattcccccg gccttcttga cggtgccgcg agcacaaggg aatagaagag cgcttccgct
     3301 ggcacgaact cctgatagaa gagtgcgcct gccgcagcgg tcttactttg ctcctcaagc
     3361 ctgatccgcg cggtgacctc ggtgccgtat ttcgcgaagt ggctgaagtt cgtatcatcc
     3421 atgatcacaa gccgcttccg ctcctcagct tccaaggcat cgaagaaatc gaggccgaag
     3481 ggcttgccat cgaaaacgaa ctcttcgaga atcagcttgc cgttgtggac acacggcgat
     3541 ccctgagcac aatgcgcggt cgattcatcg atgctcatct tcggaagcgt gactaccttg
     3601 ccaaccagcc cagcgtcacg gctccaacgc tgaagcgcct gtgggcacgt cacccatgcg
     3661 aagacccctt tcagacttcg aacgggaaac gccagtatcc gggcatccgt gaaggacagc
     3721 gctccggccg acgtgccttc ggcatctcca gcgccgaata agcgctggcg gagaccgttc
     3781 tcagtcgctt cgcgcagtac gcccttaaca ctggagccag ggatgaccgg ccaatcagtg
     3841 tgccgttccc gctgcactgg aagatccacc gctcccaatg ctgtccccga accggggtgc
     3901 aacggtgtga gcgcctgaag gtacaaaatg ccgcctacgt tattcgctgg catagttcca
     3961 cgatcctttc acaaagaatc catagcccaa agcactatct tcaccgtccg cgccgaaata
     4021 ccgatttgcc ggcgccggcc cttcgatgaa gtacaccgat cctgcatcga cggggcagac
     4081 tccgctggtc tacgctaagg cgatctcggc acggtttgat catcagcgcg ggctggctct
     4141 cggcatcgct atggcgggag tcggcttggg cgctgctctc gttccgcagt ttgcacacgc
     4201 gttgatcggg tagcgggctg gcgaggggcg tacgccggcc tcggagcgct gatcttcatt
     4261 cttgcttccc ggctgtgggg ttgtccgtgg gcgggcgggt cggggagcgg gaggccgttg
     4321 ttgagaaagt cccgaacgcg cccctgcggg gccttacggg ttttgaggcg ttgcgatccg
     4381 tccgcttctg gtctcttgcc gtcagcttct tcatcatggc cggaacaacc gggggcgtga
     4441 tctctcatct ggtcccgctt ctgggagacc gcggcatctc tgcgcaaacc gccactggca
     4501 tggtggccct cgcgggaatt gccttgatcg ccggccgtct gctcgccggc ttcctgctcg
     4561 atcgtatcca cgcaccctat gtcgcggtgc tgttctttct cgctccgctg gtcgggatcg
     4621 ccgtcctgtt atcgacgccg cggccggaag gggcagccgt cgggacgata ctcctcggga
     4681 tgggtgtcgg ctccgaggtg gatctgatcg ccttcctggt gtcgcgctat tttggaatgc
     4741 ggtcgttcgg cgagatctat ggttacttct tctcgatctt catggtggga gcaggactcg
     4801 gtccctttgc catgggcgtg tcgtacgaca ggacgcgctc ttacaaactg atgcttctat
     4861 gcttcatggt cgctctgccg ctggccagcc tgcccatgct gcggttgggc acttacgtct
     4921 atccgagtac acgcaaagcc ggccgggagc atgtcctgac gtccgctcag tattagtgag
     4981 ccacagccgg gagtcagtgc tgggggatag gcaggtccgg gcttacaagc agttggtcgg
     5041 caaccatgaa tgcactactt ggctgagttg tcgccgtccc gcgcacattc ggcagttggg
     5101 ctcgatgccg atccggtctg agacgtcggt tccctcagga gcggacgtta atcacaataa
     5161 gataatttcc ggatcctcgc ggctgagaga ctaatctgct tggcgaaagt ttgctctcta
     5221 ttttcgggag ggcgtctgcg agaaccgaag catcgttcta cagcctggcg gaacggaaga
     5281 tttcttttga gttttctgag gcaggcctca tttcctaaac actccagccg cttttcctat
     5341 ttccgaagta ctattgacct cgaaatttgc ggttcctaaa ctgaaccgat gcccacgatc
     5401 cgtattgtta gcgcggccct gcttgttacg gcgctgctcg tggctcaagc gccggttatt
     5461 ggtaattgca atgtttttcc ggcggatgat gtctggaaca cgccggtcga caagctgccc
     5521 gtcgatccga actcggcggc ctacataagc acgatcgggg cgagtctcgg actgcatgcg
     5581 gactttggcg ccggtcttta caacggcggg ccgatcggca ttccgtttat cactgttccc
     5641 ggcatgcaga ccaagtatcc cgcgagcttt ctctacgccg atgagagcga ccccggtccc
     5701 tacgcgattc cgctcaatgc gcccatcgag ggcggcagcc agagcactgg cgaccgccac
     5761 gcgatttccg ttgatacgga taactgcatc ctctacgagc tctacgatgc gtttccgcaa
     5821 gcttcgagct ggaaggccga ctcaggagcg atttttgatt tgaaatcgta tgccctgcgc
     5881 ccagccggct ggacctccgc cgacgccgcc gggctgccga tcatgtccgg cttggtccgg
     5941 tatgacgaga tcctcgcggg ggagattcgc cacgccatcc gcttcaccgt gccgcaaacg
     6001 cgccgggcgt atatctggcc ggcgcgccac tacgcatcga gcctgaccgg aacccaatat
     6061 cccccgatgg gccagcgctt ccgcctcaaa gccagcttcg acgtcagcgg cttctcgccg
     6121 acaaaccaaa tcatacttaa ggcgctccaa aaatacggca tgattctcgc ggacaacgga
     6181 tcggcttggt tcctctccgg agctccggac gaccgctgga ataactcgga tcttcacgcc
     6241 ctgggagccg tgcttggctc gaattttgag gcggtggatg agtcttcgct catgatcgac
     6301 ccgaactcgg gacaggccag acaatcgggc gtgaccgtca ccgtctctcc gccagccgct
     6361 aacatattgg taagcgctac gaagctgttt tcggcttccg tcagcggaac ctcggacagt
     6421 tccatcatct ggtcggtgaa tggcgtagat ggaggcgata gcgctgtagg ctggatcgat
     6481 tccaccggcc tgttcacggc tcccaacgca gttccctcgc ccgccactgt gaatgtgcag
     6541 gcgcggagcg tagcttcacc gacctcgatc ggatccgccg cggtcaccat cgtgcctccg
     6601 ccggcgatca gctccgtcag tccgtcgccg ctccctctcg gctccttcac gatgaccgtc
     6661 aacggaagcg ggtttcaaaa cggcgccgcg gtgaagctcg gcggcgcggc gttggcgacg
     6721 gcttttgtct cgccaatcca gctcaaggca tccggatccg cttcttccac cggttccggc
     6781 attccggtca cggtaatgaa ccccgacggc ggcgtttcga acgccgtcac ggtggatgtg
     6841 gtgaatttac cggtcacggt gaccgtatcg ccttccagcg cctcggtaag ggtgcggaac
     6901 acaaaacaat tcaccgccac cgtgcagaat tcgtccaatc aaagcgtgac ctggaaagtg
     6961 aacggcatca atgggggcaa cgcaacggtc ggaaccatca gcgcgagcgg cctttaccgg
     7021 gcgcccaact cggtgccctc gcccgccacg gtgacggtga gcgctcagag tgtggcggct
     7081 ccctcagctt ccggaagcgc atcggtgact gtcacgaagc actgatgcca ctgccagagc
     7141 gctttacctc gaagcccagc cggctctgac ccgcggggag aagcgcccgg gcccgccgcc
     7201 acaccgtcat tattaggcgg cctgtaattc cgatgtgcag ttcgggcagc gcgacgcctt
     7261 gagggggatg atcgagaggc agtacggaca gtctttggaa accggcgcag cggccgccgg
     7321 cgccggcgcg cgcttcatcc ggtttacttg cctgaccagt agaaagatcg cgaaagacac
     7381 aatcagaaac tcgatcaccg tgttgatgaa cagcccataa ttgatcgtcg ccgctcccgc
     7441 ggctttggcc gcggccaggg agtcgaagga ctgcggcgag aggttgatga acatattgct
     7501 gaaatccact ttccccagca gcaggcccag cggaggcatc agaatgtcgt ttacaaagga
     7561 cgtgacgatt ttcccgaatg ccgctccgat gacgattcct atcgccagat cgagcacgct
     7621 ccctttcatt gcgaattctt tgaattcttt cagcatggtt cagctcctag ggaagccagt
     7681 gtactactga ttgggcaaaa ggagcccttc gggagaaagg aaggattgcg ctggcgggac
     7741 ccgctctggg gggaacgcct ccgggagggg cttgaggggc gattcgaaac ttagcgcccc
     7801 tcgccctccg gaaacagctc agttgcgggc ggtctgcatc tttggcacgg ccagataacc
     7861 ggcgagctgg tctttcttca cttgattcac gacgatctcg cagggttgcc gcgcgccggc
     7921 cacataaaat tgaaccggct cgttggtgtt cttgtctttc ttctcgatcc gcttgtcgtc
     7981 cgcgaaaaca tccagggtgt acttgttgtg cttcggatcg gctttcttga gcaggattcg
     8041 aatgtcgccc accttttgcg gctgcttggt ttttaccagc gtaaattcgt aatagtcccg
     8101 ctctccgagt ctttttagcg ccgcgagttc ctgggaattc gtagcaatac ggccgctcac
     8161 cacgcccatg tcgcccgtga cccgcttcag atcggcgacc gtgctctcga gctctgtttt
     8221 cgtggccgcc acttccttgg agacgtcggc gattttcaca tcgttcgctc ccgcggcctg
     8281 tttcacgtcg ctcagctcgc tggccactcg ctcctgctgc gctttctgca aggcctgctc
     8341 ggcggcgagt ttttgcgcga gctgttccgc atgccgctgc gcttcgatct tggcctgccc
     8401 ggcgctccgg gcggcggcaa gcctggccgc acgcagttcc tcattgagag cgtccagctt
     8461 ttgccggctg gagacggatt gctccgactc ggtctcgcgc aattccgata tctgcgtgat
     8521 catcgaagcg cgccagtccg acatctccgc ttgcagatga tgcacgcgcc acagcaagta
     8581 ggcgttgccc ccgaataaga cagcaaccag cgccgccagc gcccaatggg aagtcttcgg
     8641 cgttgtctgt tccataacca tcctcctggc ctgatgaagc cgagcgcaat cgggctgcca
     8701 gtcgggcgca cggcaaaggg agtgatgcca tccaggtgaa aagaaagaag cacgcggcgt
     8761 gattgcttga gccgcgccgc gcctcgcgtg agttgcgttt gcgtattttc ttccgggccg
     8821 cgtaagattt ttaccccgcc ggcgattgct tcgccagact gagccctcaa aaaccaagca
     8881 tctgaaaaat tgtttttgcg cagccgcgga gcggcttgat gaggagggcg cgcagttccg
     8941 ggaaccgccc agcctcgcct ccgccgcctg tgcggcgggg cctatcgtct gacgatatga
     9001 gacgaataga caagctcccg ttttctcccc tggatcgtta caataggtga aggttccgaa
     9061 aaaagagcgc cggcctttcc gcgccgccgg gaactttttt ctctcaacct cgttacaata
     9121 gacgaaggtt cttgaaagac acagattgag gcggagcggg cggcggcgga ccgggtgcat
     9181 ccctggccgc gccggccgca aagcaacgtc ctggagagga gccgctattg catcccaagt
     9241 tcctgacgcc ggccgtactg tatatactca gcagcgtttt cctgtttccg ggctgcggca
     9301 atgctcctaa agaggtgcga tccgccggag cgcctccggc tgttccggtc agcgtggcca
     9361 cggctgttca gcaatcggtg ccggttgaga ttcatgccat cgggaccgtg gaagcatcca
     9421 aggtcgtgca ggtcaagtct cagatcgccg gcgaactgat gagtgtacac tttgtcgagg
     9481 gcggcgatat caaaaaaggc gacctgctgt ttgagatcga tccacggcct taccaggagg
     9541 ctctgcggca agctgaagcc gccgtagcca aagaccgggc gcaactggcc caggcagaag
     9601 cgaatcttgg acgcgaccgg gcgcagttga aattcgcgga tgccgacgcc gcgcgctatg
     9661 aggcgttgtt gcgggaaggc gttgtgtcgc gcgcccaata cgaccaggca cgcaccaacg
     9721 ccgacgctct caaggaatcg gtgcgagccg atcaggcggc catcgacact gcccgggccg
     9781 cgcttcaaag cgacctggcc gccgtggacc gggcgaagct cgacctcagt tactgtcaga
     9841 tccggtctcc gattacgggc cgtgcgggca atctgctggt gcacgagggc aacctggtcc
     9901 aggccaacgg cgacaaggcg cttgtggtga tcaaccagat taccccgata ttcgtatcgt
     9961 tcggcgtccc ggaagaacat ttgagcgcga ttcgccggag cagcagtgtg cggcagctcc
    10021 ctgtcaaagc cgctcctcaa gacaacccgg agaagaacgt tgcgggagtt ctcacggtca
    10081 tcgacaacgc ggttgattcc gcgacgggta cgatccgcct caaggcgacc ttcgagaatg
    10141 ccgagcgcct gctctggcca gggcaattcg tgaacgtggc gctcacgctg gatacaagaa
    10201 acaatgccgt cgtcgtcccc tcggaggccg ttcaggcggg ccagcagggc cagttcattt
    10261 acgtagccaa gccggatcag accgtggaac tcagaaaagt ggcggtggga atcagcgtcg
    10321 gcaagggagt agtgatcgat aaaggaattg cccccgggga ggccgtggtg accgacgggc
    10381 agctccgctt gtttccagga gcgcatatac aatccgttcc ggccagcaat gtagattcga
    10441 aagggctgta agcaggaggc gccgcaggcc ggcgcgggcg gcagagtgcg atcgcaggct
    10501 aaggaaaaac agcaggcgca acggcataac ggattgcggg cggatccggc ggccgccggc
    10561 cagatcctaa accgccgggc cggcacgctg ttccgccgta tgagcgtccg gcagccatct
    10621 cgggtgtttg tgcgatgaat gtttctcgtc tttttatcga acggccggta atgacggcgc
    10681 tggtgacctt tgcgatcctg ctgttcggga tcgtcggtta tcgcgccctg cccgtcgccg
    10741 cgctgcccag cgtggattac ccgacgattc aggttttcgc cgggcttccc ggggcgaacc
    10801 ccgagacgat ggcatcctca gtcgccacgc cgctcgagag acaattctcg accattgccg
    10861 gcatccagtc gatgagctcc gtcaacacgc agggcagcac gcagatcagc attcagttcg
    10921 tgctcgatcg caatatcgac gcggctgcgc aagacgtcca ggcggcgatc tcgacagcgc
    10981 agggacagct tcccgcaaat atgccgcggc cgccgtctta ccagaaagtg aatccggccg
    11041 atcagcccgt gctttacctg tcgctcacct ccgccacgct gccgctctac acggtggatg
    11101 agtacgccga aacgttgctg gcgcagcgca tttcgatggc cagcggcgtg tcgcaggtgc
    11161 aggtctacgg ggcgcagaaa tacgctgtcc gggtgctgct caatcccgat gcgctggtgg
    11221 cgcgcgggat cgggattgac gaagtccaaa gcgccatcca aaagagcaat gtgaacctgc
    11281 cgaccggacg gctatggggc gagaagcagg cgttcaccgt ccagtccagc gggcagttga
    11341 cgagcgcggc agcgtaccgc ccgatgatcg tggcgtaccg gaacgggctg ccggtgcggc
    11401 ttgcagatct gggcagggtg gaggacagcg tcgagaacga caagaccatt gcctggctta
    11461 atggcgtgcg cggggtgatt ttggccattc gccggcagcc cggcaccaac acggtcgagg
    11521 tggtggacaa catcaaggcc ctgctgccga tattccgcag cgagataccg ccggcggtcg
    11581 atctctcggt ggccttcgac gcatcggagt cgatccgggc ttcgatcaac gatgtgaagt
    11641 tcacgctggt gctgaccatc tgcctggtcg tgatggtcat ctttctgttt ctgcgcaacc
    11701 tttctgccac gctgattcct ggcgtcgccg tgccgctctc catcatcgga acgttcgccg
    11761 tcatgtacct gctcgggtac agcttgaata acctgtcgct gatggctctg acgctgtcgg
    11821 tcggcttcgt agtggacgac gccattgtta tgctcgaaaa catcgtccgc tacatggaaa
    11881 tgggcaaacc gcgcctggaa gctgctgtgc ttgcttcgcg ggaaatcgga ttcaccatcc
    11941 tctccatgac gctttcgctg gtcgccgtct tcattccggt cctgtttatg ggcggcatgg
    12001 tggggcggct gctgcacgag ttttccgtca cgattacggt cgcaatcctg gtatccggtt
    12061 tcctatcgct caccctcacg cccatgctcg gcagccgctt tctgcgctac aggcaggaag
    12121 gccggcacgg aacaatgtac cgggcgctgg aagccggctt tgaggctctg gcgcgaggct
    12181 acgattacac gctgcaaagg gtgttgcgcc gccagttggc gaccctaacc gtcgctgtag
    12241 cgctgctggc gggaacgctc tacctgttct tcaccatgcc cacgggcttt attcccagcc
    12301 aggacagcgg attcattttc ggcgtcacca tggccggcca ggatatttcc ttcgaatcca
    12361 tggccaaaca tcagaaggcg gtggccgata ttgtccagag cgatcccaac gtccgcaaca
    12421 cgggggcctt cgccccgccg gggaaccagg gattcgtatt catgaacctg aagccgcgct
    12481 ccgagcgccg cctcaatgtt gaccaggtga tggatgagct gcggcccaag ctcgctcagg
    12541 ttcccggcat gctgacgttt cttcaaaatc cgccgcccat cacgatcagc ggccagttca
    12601 ctaccagcgt ttactccatg accttgcaaa gcaccaacct tcaggagatc tacacttggg
    12661 tgccgcgttt gttagcgaag atgcgggcgc tgcctggatt tttggatgtg aattcggatt
    12721 tacaaattgc cagcccgcag gtgatggtcg atattgatcg cgaccgggcc ctggcgctcg
    12781 gcgtcacgcc ccagcagatc caggacgctc tctataccgc ctatggcgac cgccagatct
    12841 cgacgatata cacgcccgct aatcagtacg aggtgattac gcaggtggag ccgcaatacc
    12901 agcgcacgcc agacgcattg tcgaaactct acatacggtc ttctcaaggc caattggttc
    12961 ccctcgacga ggtggtaaac atcacccgca acgtcggccc gctgagcgtg aaccattttg
    13021 gccagctccc ggccgtcacc gtctccttca acctaaagcc cggattctcg ctcggcgaag
    13081 cggcccagcg ggtggacgag gcggtgcgcg agctgcgcat gcccgccacg ctcagcgtca
    13141 gcttccaggg cacggtgaag gaatttcaga aatcctttca ggggctctcg atcctgctaa
    13201 tcgtcgcaat tctcgtgatt tacattgtgc tcggaattct gtacgagagc ttcattcatc
    13261 ccatcactat tctttccgga ctgccctccg ccgtcttcgg cgcgctgctg acgctgaaac
    13321 tgttccacaa ggagctggat ctttacgcct ttgtgggttt gatcatgctt ttcggggtgg
    13381 ttaagaaaaa cgccatcatg atgattgatt ttgctctgga agcccaaaga accgaaaact
    13441 ccggcgcaag ggacgcaatt tacaggggct gcttgctgcg gttccgtccc atcatgatga
    13501 ctacggtcgc cgcgctcttc ggcacgctgc cgatagcgct ggcctacggc gaaggcgccg
    13561 acgcccgcca gccgctcggc ctggcggtgg tcggaggcct cctcgtgtcg cagttgctga
    13621 cgctttatat cacgcccgtg atctaccttt acatggagag atggcaggcg tggctgcgag
    13681 gcgaaggggc ggtgcgcgag ccccgggagc ctgcggtcgc gaaggcgtga gccggtgcgc
    13741 cgaagagtgc gcggaaccac ttcgcgccga aggcgtcaag gccgcccagc ctatgccgca
    13801 tcatagcggg ttgtgccgca cttgattttc cccttgaggt acaatcgaag cctcttgtat
    13861 gagcgatcga cccgcgaaca ttcacatcgc gtgggctacc gagcgggaag cgcctgtgat
    13921 tctcgatttg atccgcgctc ttgctgagta cgaaagactg acccacacgg ttacggcgac
    13981 cgaggagcaa ctgcgcagga ccttgtttgg agaaagaccg tgcgccgagg cgctgcttgc
    14041 gtcgatggaa ggcgagcacg tgggctttgc cgtcttcttc acaaattatt cgacgttcct
    14101 cgcccggcct gggatctatc tggaagacct tttcgtccgg ccccacgcca gaggaaaagg
    14161 aattggcgcc gccctgctgg cgagtgtggc gcgcattgcg gtggagcgcg gatgcgcccg
    14221 aatggagtgg tctgtgctca actggaacga gtcttccatt gagttctaca aaaagctcgg
    14281 cgccgcgcca atgaatgagt ggacgatcta tcgtcttgcg gctccaggca tcgaagacct
    14341 cgccagccgg ttcccggcga gcttctccct gctgcgtagt tcccggcgtt aagagtaaat
    14401 ctgtacgcgc agcggcgaat tccgggttcc cagccaggag gatgagcgtc gtccgaatgg
    14461 gtcgagggcc gggctgaacg gtgctgcttt tttcgaacgg gccagcctgt tgttccctcc
    14521 tcacatcaag ctcatgggat ctacatcgat gaccagcgcc gtcgcgggcc acttttgctc
    14581 ttcggcgaag cgccgggcgc cttgcagcag ttgcccgaga tcgcggcggc tggacgcctt
    14641 caacagaaat tgatagcgat actcggcctt cagtttcacc attgccgccg ccgcgggacc
    14701 cagcacccgc accgtcttag gcgaattctt gagatattcg ccgagcatgc cgctaagctt
    14761 caaggcctcc tccagcttcg cgctgcgcac caggatattg gccagcgcgg cgaagggcgg
    14821 atagtgcaga aagcgccgga agttcaattc cttttcataa aatccctggt agtcatgggc
    14881 ctttgcgaac tggatgacat agtgatcggg gttgagagtc tggatcagca ctcgaccggg
    14941 cagctcgccc cgtccggcgc gccctgccac ttgagtgagc aattggaacg tgcgctcggc
    15001 ggcccggaaa tcgggcagcc ccagcccgat atcggcggaa acaatgccga ccagcgtgac
    15061 gttcgggatg tcgtgcccct tggcgatcat ctgtgtgccc acgagaatgt caaaactgcc
    15121 ttcgcggaag ccgcccagaa tcgactcata gcgccgccct ccccgtgcgg tatcacggtc
    15181 gagccgcgcg atgcgcgcgc gggggaaggc ctcgtgcagg gtctgttcca ctttttccga
    15241 gccgctgccc aagaagtaga ggtattcgct gccgcatttg gggcacgcgg ccggcggccg
    15301 ctccgcataa tcgcaatagt ggcagagcag gcgctgctcg cgccggtgat aggtaagggt
    15361 gaccgagcag ttctggcact cgatccgctc cccgcaactg cggcaggcga caaagctcga
    15421 gtagccccgg cggttgagca ggatcatgac ctgctcgccg gcggcgaggc gcgcttcgat
    15481 cgcgtccagc aggcaacggg aaaacagccg ctgctggcgc gtctcgatga actccagccg
    15541 catatcgatc agatcgactt cgggaagggg ccgcttctcg atccgctctt ggagctcgat
    15601 cagcgcgtac ttgccgcgtg ccgcgttata acgggattcc aggcagggcg tcgccgagcc
    15661 taacacgacc gtcgctccgg cccgctcgcc ccggacgatg gcgacatcgc ggccatggta
    15721 gcgcggcgtc tcctcctgtt tgtaactcgc atcgtgctct tcatccacca cgaccagacc
    15781 aagattctgc aacggggcga ataccgcaga ccgcgttccc actacgaccc gcgccgcgcc
    15841 ggagcgaata cgagcccatt gagcggcgcg ctcggcggcg ccgaaggcgc tgtgcagaat
    15901 agccgcttgc tcgccgaacc ggcggtgaaa cagcgctgcg gccgcgggag tcagactgat
    15961 ttccggcacc agcaggagcg cgctcttgcc caattccagc gcggtttcaa tcgcgtggag
    16021 atatacttcc gtctttccgg atcccgtcac gccatgcagc aggaaggtct gaaatttgtt
    16081 cgcccggatc gacccggcca cggcctccag cgccgcgcgc tgtccagcgc tcaactccgg
    16141 cttgggtgca ggctcggccg gcgccagctc ttctcccggc tccttccaca gccgaaggat
    16201 gcccgctttt gccaggcggc gcgcagcggc ctcgaatccc tggacgtcgg cttcgagcgc
    16261 ttccacctcg tggggacccg gatgcgcggc cagaaaatcc aaaagccgct tttcgatctt
    16321 ggagaccttg cgaggaaaat ccctgtgcgg gatgctctcc actataaggc gtccattgtg
    16381 agcgcggagc gggtcccgca gacgctcctg ccggtcaata cggatgaggc ccttccgctc
    16441 cagccctcga atgacccctg cggccagcgg cagcttcttt ttcagatagc tctccgcgag
    16501 cgggcggcgc tccagcgcac gcagaatctc ggcgaccggg tcgtcggctt cggcaaacat
    16561 cgagtactgg cgagctgctt cctggccggc gggagtcagt gaaacaaggc gaacccgccg
    16621 cacgtcaatg gcgggcggca gcatgacccg cagagtctcc cccagcggcg cgcggtagta
    16681 atcggcgatc cagcggccta aatcgagcag ctcacgagaa agcgtcggct cctcgtccac
    16741 cagccgcagg accgctcgag cttccgcagt aggttggcga tcgtggcatt cgaggatcac
    16801 tccggtcagc ttgcgcgctc cgaacggcac gataacccgg catccgggct gcgcgctcgc
    16861 ttccagggca ggcggcagga ggtacgtgaa gggcgtgtcg agcggaactg gcagcgacac
    16921 gtcgcagtac agcggcatgg gcctactgat tgtatagcaa gcggatgccc cggcgagccc
    16981 cggaactgct tttcaggaag gacgccgttc gccccggggg cattcctgcg ccggctcctt
    17041 cctctgcccg gcggctccgg cggagtgctg aatcctccgc cctgccatcg cccgagtcgt
    17101 ttctcccttc ctgtgcgaaa ctgaagtgcg tatgtttcgc ctggattccc atgagtggct
    17161 gatcgtagtc tccgttgctg ctttgcttgt gcccgttctg ttgtttcccc aggccgcgag
    17221 ccggtctgtc cccacagcta aggctctcaa ggtcaccccc gacctcgagc agcggctcgc
    17281 caaatggcag ccagtaagga tgcccttcaa cgccgctggt ttgagtgtgc gcgaacgcca
    17341 gatgattgaa aagctggtcc gcgcctgcca gtatctcgac gacatttact ggcggcagag
    17401 cgacccgcaa ggactggctc tctataaatc tctcgccgcc agcaggactc cgcgcaacca
    17461 acaattgcgc cgcatgctga tgatcaacgg agggcgcttc gatttgctgg atgagaaccg
    17521 gccgttcgct ggaaccgagc cgatgccgcc cggccgcggt ttttatcccg aggggctgac
    17581 ccgagagcag atcgagaagt atgtccagca gcatcccgag aagaaggctg aaatctacag
    17641 cccttacacg gttgtgcgcc ggcggggccg ggaattggca ggcgtcccgt accatcaggt
    17701 atacaaagct ttcctcgacc cggccgcgca gactttgcgc gaggccgcgg cgctcagcga
    17761 tgatccggcg ttcgctcgct tcttgcagct ccgcgcagcg gccctgttgt ctgatgatta
    17821 ctacgagagc gacctggcct gggtcgacct ggaagatccc aaattcgatc tgatttttgc
    17881 tccgtatgag acctatctgg atgacctgct cggggttaag ggctcctacg gcgcagcagt
    17941 gctcattcgc gatgaggagc agagccggaa actggcagag tatcagaaat atgttccgga
    18001 gattcaggat gcgctgccgc tgcccccgca agaccggcct tccaagcgcg gccattccac
    18061 tcccatggag gtgatggatg cgccgttccg ggccggcgat ctccggcacg gctaccaggc
    18121 tgtggccgac aacctgccca atgatccgcg catccaccag gccaagggca ccaaaaaaat
    18181 ctttttcaag aacttcatgg atgcccgcgt caactacatc attctgccgg tcgcccgcct
    18241 tctgatgcgc cccgaccagg ctgccaaagc ttcggccgaa ggctacctga cgtttgtgat
    18301 gctgcatgaa atctcgcacg gcctcggtcc cgcctacgcc cgcaaacagg gcaagcaggc
    18361 cgacattcgc gaagcgatcg gcccgatttt ctcagctctg gaagaggcca aagccgatgt
    18421 cgttgggctg tttggtctcc actggatgat gcggcaggga gccttgccca cccagcgcag
    18481 cgaagaatac tacgcgtctt atgttgccgg agttctgcgc agcgtccgct tcggcccggg
    18541 tgaggcgcac ggccaggcgg agatgatgga gttcaacgtg ctggcccagg aaaaagcggt
    18601 ggcgcgcgat gcttccaccg gccgctacgt catcgactac aggcgcatgc cgggagccct
    18661 ggaacggctg gctaaggaac tgctagaaat agaagcttcc ggggaccgcg accgcgcgga
    18721 aaactggttt cagcggtatg gcgcgatgtc gccggaatta aaagaggctc tccaggcggc
    18781 gagcgggatt ccggtggaca tcgatcccat cttttcgttt gcggagccgg tccgctgagg
    18841 ggacttcctg tgggcggcca aaaacgtctc agcgcagggt caaccgttgg gcgatggcga
    18901 ccaagtcgga cggattgtca atccaaaaat caggatgcca gcttctcaac tccgcacgat
    18961 ctccataacc ccaacccacg gcgcaggtta gcattccggc ccggcggccg gcttccatat
    19021 ccaccggggc gtccccaatc atgaggcatt gctccgggcg aaccttcaac gcttccgccg
    19081 cttccagcag aatcgtggga tcgggcttgt agcgcccgtt gtcgctgccc tgcacgtgat
    19141 caaaatgccg gcgcagtccg aataattcaa ggaccaaggc cgtcgtggcg ctgcttttcg
    19201 tcgtagccgt gcttttgagg ccgggcaggg ccgcgagcat ttcctgaatt cccggaaaca
    19261 cgcgcgtccc tctatgggcc cgctcgcggt attgctcgcg gtagagcagg cagaggcgct
    19321 cgatttcgtt ttcggatgag ccgggataaa tctcggcgaa ggtttccgcg aggctccggc
    19381 cgatgtaacg gcgaagcacc ccctcggata cttccggctt ttgagccagc gagcacgccg
    19441 ccaaaataga gctgcaaatg tcggttgccg agtcgaggat cgtgccgtct atgtcgaaaa
    19501 agaaggcttt aatcaacaat cgagatagaa gggaaagccc taagaatttg catccagagt
    19561 tcagatgccg gaatgctctg cttcccgaga ttttcacgcc ggctgcgggc gttccccttc
    19621 ggagaggccc ggaatccgct tgcctgtatc gggccggagc accttctgcg tcggctcctc
    19681 ttcggccggc gtgcgcgggg gcgctggagt gatcgaaggc agtgtcttgc cctcgataag
    19741 caacttgatt tccgcaccgt ccagcacctc gcgctcgaga agcgcttcgg cgatacgcac
    19801 cagggcgtcg gaatgctcct gaatcacctg cttggccctc tggtagcccg tggtcaccag
    19861 ccgtttcacc tgagcgtcaa tgtggactgc tgtatcctcg ctgtaatcgc gatgctgggc
    19921 aatctcccgg cccaggaaga tctgctcttc tttcttgccg aatgtcagcg gccccatctc
    19981 ttcgctcatg ccccactcgc agaccatgcg ccgggccatt tccgtcgcct gctcaatatc
    20041 gttgcccgcg ccggtggtca tttggttgag gaaaatttcc tcggccacgc gcccgcccat
    20101 caggatcgcg agctgcgact cgagaaattg ccgcgagtag gtgtgcttgt catccatggg
    20161 caactgcatg gtgaggccca gggccatgcc gcggggaatg atggtcacct tatggagcgg
    20221 atcggcatgc ggcatcatcg cggcgaccaa agcgtgtccc gcctcgtggt aggcggtcgt
    20281 tttcttctcc tcttcgctga gaatcatcga gcggcgttcg acgcccatca acaccttatc
    20341 cttcgacagc tcgaagtccg acatggtgac taccttgcgg ttctggcgag ccgcgtgcag
    20401 cgccgcctca ttcaccaggt tggccaggtc ggccccggaa aagccaggcg tgccccgcga
    20461 aatgaccgtg agatccacat cgtccgccag cggaaccttc ttcgtgtgca ctttcaggat
    20521 ggcctcgcgg cccttcacgt caggccgcgg caccacgacc cgccggtcaa agcggcccgg
    20581 ccgcagaagc gcggggtcca gaacgtcggg acggtttgtg gcggcgatca aaatgacgcc
    20641 ttcgttcgag tcgaaaccat ccatctcgac tagcaactgg ttcaatgtct gctcgcgttc
    20701 gtcgtgcccg ccgccgagac ccgcgccgcg gtgccgtccc acagcgtcaa tctcgtcgat
    20761 gaagatgata cagggagcgc tttttttgcc ctgatcgaat agatcgcgca cgcggctggc
    20821 gcccacgccc acgaacattt ccacaaaatc cgagccggag atcgagaaaa acgggacatt
    20881 ggcctcgccg gcgatggccc gggcgagcag agtctttccc gtgcccgggg acccgaccag
    20941 cagcacgcct ttcgggatcc ggccgccgag tttttgaaat ttctgaggtt cccgcaggaa
    21001 ctcgataatc tcctggagct cttccttcgc ctcttccacg cccgccacgt ccttgaatgt
    21061 cacctttttt tgctgagtgg agagcaagcg cgcccggctc tttccaaagc tcaaagcttt
    21121 attgccgccg gtctgcatct gccgcatcat gaagatccag aatgcgatca gcaggataaa
    21181 cggagaggcg ttgacgagca gcgtgaccca gttattcgag tccgcctgtt tcacgtcgat
    21241 gttgactttc ttttcctgta gcagcttata aatgtccggg taattggtcg ggatcgtcgt
    21301 tcggagggtg acgctgctgt tcttcagctt gccggtgacc tggtttccgt tagaaatcgt
    21361 gacctcgctc acattgccgg cctgcacttc atcgatgaac tgagtaaagg tataggtctt
    21421 ctcgcgcccg gggctgccgc ttttcacgac ggaccagagc agcacggcgg tgcaaatcat
    21481 gaccacccag aagattgccg tcttaatggt tgaattcaca ctacgtctcc aattcgatta
    21541 gacgcttccg ccccgctctc ggtcgatttt tcttgcccgc tttcgctttc cgtaatttct
    21601 aggacagtcc ggcattccgg cccggccgct agttccgctg cgacgccaaa cctccgggcc
    21661 caaacaattg cggaccccgc tgcgacgacc ggccaatgtc ggcgctccca aaccgggatt
    21721 cgatgctgtt gaaagagggt cttcagcttt tcctcgccgc tccagcctcg gggatggtac
    21781 cgatcgcccg gccgccagtt gcgcagccac aacgggcgcg gagtcctctc ccagtcgaga
    21841 caaccgacgg tattattata acctcgatcg ctgtcccggt ctgtacccaa tttgagaata
    21901 agacgcgtcc gtccccccgg aatctcgtag gtccctggca cagacaaggg aaccgagtaa
    21961 tttctgtcct ccaggccctt gacactctcg accggcgcca gccggatctg agagaacgac
    22021 cgaaacacgt ccacgccggg aatgcgcacc cgcccatctc cctccggtaa agctgccagg
    22081 gagcggattt gctcgatgtg agcgaactcg atgcctcgca aatccccccg caccctttcg
    22141 acagcatggc gcagcagccg gcgcgcgacc gccgccggca gggctgcgat tccgggcgca
    22201 tccaaataca gcatcggccc ttccagccgc atatattgtg gagccagcag cccgatttcg
    22261 ttccgccagt acgcttcctc atcgcgcgcc caatccgcaa gctgggctaa ggtattgatg
    22321 atagaagggt tccactccct ttccaggcgg ggcagcaggt ccagccgtaa gcgattacgg
    22381 gctatgctga gatcccggtt cgacgaatcc tcgcgccagt tctgccccaa acttcgcaag
    22441 tagtccagaa cctctcccct ctccacctcg agtaaaggcc gaacgatgcc accctggaaa
    22501 acgggataaa ttccggccaa gccggccgtc gccgagcctc gcagcagccg gaacagcacc
    22561 gtctcggcct ggtcggattt cgtatgcccg acggcgattt tctggaattg gttttcccgc
    22621 aggagccgct ggaaatagcc gtaccgcagg cgccgggcgg cttgctccag gttttctccg
    22681 gttttggaga tttcgtcggc gagacgagcc gtttcgagcg ctagttccag gttcagcgac
    22741 cgggcgagat cgcgcacgaa ttccgcgtcg gccttggatt cggcgcctcg caggccgtgc
    22801 tctaggtgaa ccaccgcaag caggacgcca agttcgctct gcaactcgcg gagcactcgc
    22861 aataatccga cggaatcggg tccgccggac acagccacac ccacgcgctc gccggcccgg
    22921 agcatcgagt atttccggat ggtctgggca acgcgttgga tcacaatttc attgtagggc
    22981 tgaaaaagag ggtgacgcgt tcgtggccag ttcgttgtag catttaagct gttggcgatt
    23041 agccggggaa aaacgctgta ggtcttgtca gaaacgccgc tggcgcctgc gtgcccatgc
    23101 gaacttctac cgcctttctg gagacttctg caagaacctg gccgatttga catctttcat
    23161 cgcacgcgtt cagaagctca tcgctggtcg gggaagggtg gcggtaaaga cgtgtgatta
    23221 cgccttcctc gcatgcttct agtgcggtca ccaggccggc gtcttcgagt acaggcgaat
    23281 cgtctcggcg cctaacaaga taccgaggcg tcaagcacag cttgtcagcc cctaatgttc
    23341 aatccctcaa ctcgctgcgc cggcaaatcg cctagcgatt tccccaggcg cattcaggaa
    23401 accgctttct ccgctaacaa ccgctgcacc aattcctcaa tttcccgctt caatcgtgcg
    23461 tgctcctcgc cggtggccgg gctggcgaag ccgtcgtgga cgcttctgac gcgcccgtcg
    23521 cgtccgagga aaatcgaggt tgggtaggaa tcgaaattga ccagttgcgg cagagctttg
    23581 ggaaccgccc ccggctccac tacgccggcg agcagcatgg gataacggat gccatgccgg
    23641 cgcataaacg cccggacctg gctgcggtcg atttccaaat cgccggattc aaagcagaga
    23701 cccacaatct ccaggccgtc atcatggtac ttccgataca attcggctag aaacggtcct
    23761 tcatcctggc agttcgggca ccagcttccc ataatgttca cgatcacgac tttgccccgg
    23821 aaccgcgagt cggcgttgga gacggtgcgg ccctccagat cgggaaactg aaaccgcagc
    23881 ggctcgctgg ggtcctttac gctggtgtac cgggatgggt ccggcggcaa tggcaagcct
    23941 ctagccaacg cctctgattt gcgaacagcg gtaaatttgg tccggcggtt tagcgtacct
    24001 tccagcgatc cgtcgggatg caacacggct tccagcagca tgggacgggc gccggagaaa
    24061 tggctcaaca cgagccggcc gttctgcaat ctgccgctga gcgtacccgt gtcgccgtca
    24121 agccgtaaga tggacgcgct gatttccgcg ccggattgcc tcaccagaaa gcgccatacg
    24181 ttcggccggc cgggatgaat cgtgccgtcg gaagtgagga tccattcacc tgccagggac
    24241 ggggcgggcg tttcgagggc ggttgcaggt acaaaccggc gggcgcggaa tgggtagctg
    24301 gtagcgccgg ttcgaccctt atggtcatag gagccttcca gcttgccgcc atgcagcgat
    24361 gcccgaaggt cggagttcca ataatcgaag cgcaggtgga tgtgatcacc ttccatctcg
    24421 ccgctgctgg atttttcttt cacatcgccg tttaagaaag cccctcgcaa ttccttcccg
    24481 gcctgggaaa actcgattcg gaacggaatt tcgagatcgt tcaccgcgac gacggcgtcc
    24541 cacactccgt cgatggaccc cccaggcgca gccgcgcgcg gagccgcact gagcgcaatg
    24601 taagcgccgg cgcccgttac gagcaagagc aaaaccaaca ggctgcctct gcgattcggt
    24661 tgcatctttc gccccttcct aacagcgcag gccggaaaca gaatcggccg gagcgctgat
    24721 cccggactgc ggccttcatt atatatccgg cctgaccggg agaatccccg ctgcggcacg
    24781 caagctgcgg ttccagccgc tcggtcgggc gccccttgag gccttccccg cttctcgaac
    24841 cgaagcagcg ccgctgccgg ccaaccgagc aagctaccgg atgccgctcc aggcttcgca
    24901 gatcacgatc gaattggagg gattgcccgg cagatacttt acgcccaccg gcccggccag
    24961 gcgcagcgga tcgcggtcga tgtaggccag caacgaagtg atgtcttgag ccgcggaata
    25021 cgtcaccccg ttcagcgtat agctaaaatg caggacgtga acggtagcgc cgccaggagt
    25081 atcgaacact tctacttccg tgattgatcc gtcagtaata cggcccgcgg cgttcaccgc
    25141 cgcccggcgg cgccgttcgc gttcctccgg agtggcggga ttacgaaaaa gcaggtagca
    25201 cagaaaaagc agcagaacgg cggcagcgga ggcagccgtt ccgagccaca gagaccggaa
    25261 caacctctac cccacggtcg tttcctgaaa agccttctgg taatcggcga ggaaagcggc
    25321 cagccccttg tctgtcagcg gatgattgaa cagcgagtcg agcactttga acggcattgt
    25381 agcaatgtgg gctccggcca gcgcgccttc cagcacgtgc agcggattgc ggatcgaggc
    25441 gaccagcact ttggtcggat acccgtaatt ctggtaaatc ttgacaattt gccggatcag
    25501 ctccatgccg ttagtcgaga tgtcgtcgag ccgccctacg aatgggctca caatataggc
    25561 gccggccttg gccgccagca gcgcctgagt tgcagtaaag cagagggtca cgtttacccg
    25621 gatgccctcc gatgagagcg cctttgtagc cttgatgcct tcccggatca aggggcattt
    25681 tacgacaaca ttcggatgaa tgcgagcgag cgaccggcct tcggcgatca tttccggcgc
    25741 tgtggtcgcg actacttccg cgctgatgtc cccatccaca aggtcgcaga tcgcgcgaat
    25801 ctgttccgca ttcgagcgcc cttctttcgc gatcagcgag gggtttgtag tgacgccgtc
    25861 aaccaggcct aaatcgacgc cggcctggat ttccttgagg ttggctgtgt ctaaaaagaa
    25921 tttcattacc gaagtctcct catttctgtc cgggctcacg ccccggcgcg gcagcgccgg
    25981 gcgttaacga tcttccgccc gcttgcggcc cgccggcttt ctgccagccc ggcggaggga
    26041 ttaacgccgg ataacgcggt ttcgcagaga gccgatgccc tcgatttcaa cgaccacttc
    26101 ggatcctggg cgcatcggcc cgacgcctga gggcgtgccc gtcgcgatga gatcgcccgg
    26161 ctcgagcgtc atcacctgcg atataaaggc gattatagca cccaggggga agatcaaatc
    26221 gcgcgtgttg ccgttttgct tcagctcgcc gtcgagatac gtccgcacag ggagttggga
    26281 agcgtcgacc tcggacgctg gcacgaggta gggcccgaca gcgcaaaatg tatcgaagcc
    26341 ctttccccgg gtgaattgcc catcccgctt ttgaaggtcc cgggcggtga catcgttgag
    26401 acacgtgtaa ccaaaagcgt gcgccagagc ggccgccggg tcggccacct gggaacagcg
    26461 ccgcccgatg acgatgccca actcgccctc gtagtcgacg cgctcggatt gaggcggata
    26521 caaaatggcg tcgccggaag cgatcagggc ggaaggcggc tttagaaaca gtaggggttc
    26581 gctgggcagc ggattggaaa actcgcgggc gtgctccgca taattacggc caatgcagac
    26641 gatcttggaa ggtgagcacg gaggaagcag ccgcacctca tcgggcgagt agaggcggtc
    26701 ggacggaatc cactggtcga gcggatcgcc ggtgatttgc cgataccggt ctgcttcgac
    26761 gatgccccac cggacgccgc ctgcgccgtc gcggatggtg gccggagctt gtttgtcaac
    26821 tgcaaagcga acgaatctca taagtgccgg ctgcgcaacc ccctcgctgt tcttacggta
    26881 tggctacttc gagcggagcg ctggggccgc tctcattccc cttgtcgtcg acagcggtga
    26941 cggtgtagac gtagcgccgt tgccgctgaa cgtccttatc gctgaagacc ggcgcgctca
    27001 acagctccgc attgagccgt acaggcggac tcggggcgga tccggagagg acgcgacggt
    27061 aaatattgta gcctgcggtg tccggctcag ggcttaaatt ccaggaaatc tcggcggaag
    27121 tttccgtggc gacggccctc agcccggccg gcggcgacgg tggaaaaacg tcgtggggca
    27181 ctatggccgc caccggcgaa tctgcgcttt cgtcgatgcc ggtcgagacc tcggcgtacg
    27241 cccttacgaa gtacgcataa ggttctcccc attcgaaatt ggagtcctca aacagtggcg
    27301 cggttgtcga gcccaggtag gcgaattgcg ggttttctcc agagcgcttg taaacgcggt
    27361 atccggcggc gccactgaca ggcgcccact ccagacgaat ggccgccggc tgcaccgttg
    27421 cagagagctt cggcggcggg cttacgagcg gcccgatttg gactatcgcg atgttggaaa
    27481 cgcccagcgt gcgcccgcgc gcgctgaaat tcttgaccgc cagggcgagg cgaactcctg
    27541 gagaagcggg gagcggcaaa tggcgctcga ctttttgtcc cccggcgaga tcgttccagg
    27601 ttgccagctc tcgggcgccg gtgtcaaacg aggcggcgtc caccgatccg gtttctacca
    27661 ccttggccag caaactggtg cgatcgacct gtttcaaggc gatgccttcg gttgtctggc
    27721 cgggcgccgt ccactggagg atcagttccg cgccgcgctg ggaagcttga agatccgctg
    27781 tccgggcggg gatattcagc agcggcggca tcgggtctcc gatcgccccg catccgctga
    27841 gtgtgaggag caaaacggca agaacgcctg cgccaaccgc cggaagaagc gaatctggcc
    27901 ggacagtcag accggcgcag cgcagcaatg ccgcggcccg ggagcggtta tgggaaacgg
    27961 gtgtgcggcc ggggaagggc gcgccggttc ccgcgacgcc gcctcggttt cggggtggca
    28021 tcagaggata tagcggctga gatcctggtc cttgacgata tcggccagtt gcttgcgaac
    28081 gtagtcggcg tcaatccgga agttcttctt cttcaaatcg ggcccttcaa acgatatttc
    28141 ttccagcagt ttttccatga tggtgtggag ccgccgggcg ccgatgttct ccgcgttctc
    28201 gttgaccttc gccgcataat tggcgatctc caggatcgca tcattgccga tggtgagctt
    28261 gatgccctcg gtctcgagca gggcggtgta ctgcttgacc agcgcgttct tcggctcctg
    28321 caaaatccgg acgaaatcgt ccacggtcag cggcttcaat tccacacgaa tcgggaagcg
    28381 tccttgcaac tcagggatca ggtcgctggg cttggaaacg tgaaatgctc ccgccgcgat
    28441 aaacagaatg tgatcggtac ggaccattcc gtagcgggta ttgaccgtcg tgccctcgac
    28501 aatggggaga atatcgcgct gcaccccctc gcggctcacg tcggggccgt ggccgccctc
    28561 ccgcccggcg atcttgtcga tctcatctaa aaagataatc cccgactgct ctacacggtc
    28621 tacggctatg cgctgaatct gatccatatc gatcagcttc tgctcttcct cctgcaccag
    28681 gtaatcgagc gcctcagcca cccgcatctt gcgctttttg cttttctggc cgaacaggcc
    28741 ggggatcatc tccttcaggt tgatatccat ctcttcgacg ccggtgttgg tgatgatctc
    28801 gaaggagggg aagctgcgct cgcgcgtctc gatttccacg atccggtcgt ccagcttgcc
    28861 ctcgcggagc tgcgtgcgga gcttctcccg gctcttctgc acgtcggcgc cgtcggcctg
    28921 cggctgcggc gggatcagca ggtcgagaat gcggtcttcg gcgttggcct ctgccttctc
    28981 ggccaccaga tcgagctttt cctcacgcac catgtcgata gcaatctcca ccaggtcgcg
    29041 gatcatggaa tcgacatcgc ggccgacgta gccgacctcg gtgaacttgc tggcctctac
    29101 cttcaagaac ggtgagttcg ccagcttggc cagccggcgc gccagttccg tcttgccgac
    29161 gcccgtcgga ccgatcatca gaatgttttt gggcatgacc tcttcggcca tttccggctg
    29221 gagtttctgg cggcggattc tgttgcgcag ggcgacagcc accgcgcgct tggcctcgtg
    29281 ctgcccgacc acgtgcttgt cgagctcgcg gacgatttcg cgcggcgtca gctcgtccag
    29341 cgccggagta tcatccacgg attgactggg aagatagata accatagaaa aacagctttc
    29401 cgagactgcc cgcagcggtt aaacgcgcca ggcagcgctg cggccgcctt tgggctggaa
    29461 tgccgctgtg gcgcccggag catgcaccgg ctgcggcagg ctcgatctca tccgatctct
    29521 tcgaaggtga cgttttggtt cgtataaata cagatcttgc cggcgatttc cattgcttcc
    29581 tcggcgatct tgcgggcgtc gagctcggtg tgcttcatga gcgccgtagc tgcggccacc
    29641 gcaaacggcc cgcccgaacc gaccgccgct acgccgtcgt caggctcgat tacgtcccca
    29701 ttgccgctca cgataaaagt gtacccggcg tcagccacca gtaagagggc ctccaggtgc
    29761 cgcaaaacct tatctgtacg ccagtctttg gccagttcca cgacagcccg gctcaacttg
    29821 ccttggaatt gttccagctt ggcgtcgaag cgctcgaaca acgaaaaagc atcggcggta
    29881 gacccggcga agccggcaag cactttgtcg ttataaagcc gccgcacctt gcgagccgag
    29941 tgcttcagaa cctcgctacc cacggtgacc tgcccgtcgc ccgccatgac cactttcccg
    30001 ttccgccgca cgcacaaaat cgtggtggag cggacccgtt gtccaatttt catgctttca
    30061 ttttcactat tcagcgatca gctccgggca aaccaagttg ggtgcggcga ggcttttgcc
    30121 gggagctgat ctctgattgc ttaagggtct ctttccctat tgtacgccag acgccggatt
    30181 ggccgtgcac ggacgcgcag cacaggttct tcttcgccag cacgagccgc gcgcaggacc
    30241 ctcgttgcgc cgccctaccc tggcggctac actagtctgg aaaggagcgc ccgtgctcgt
    30301 cgtaatggac gcccacgcga ctcaagagga tgtcgcccgc gtgtgccgca aaatcgaaaa
    30361 gctcggattt cggccccacg tcatgcccgg cgcgcagcgt acggctgtag gcatcacggg
    30421 aaatccgggt ccgatcgacc cggcggaatt cgaggacctg ccgggagtgg ccgaggcgat
    30481 tcgcgtatcg aaaccttaca agctggtgag ccgtgaagtc aaacctgacg atagcgtcat
    30541 cgagatcgcc ggcgttcccg tcgggggaag cgctctcacg ttgatcgctg gcccatgttc
    30601 ggtggagagc cgcgggcaga tccttgctgt ggcccgcgca gtggcggccg ccggcgccca
    30661 gttgctccgc ggcggagcat ataaaccgcg aacctcgcct tacgcctttc aaggactgca
    30721 cgaggaaggg ctgcaactcc tggccgaggc gcgcgctgca accggcctgg gcatcgtaac
    30781 cgaagctatc gacgtcgaaa ccttcgatct cgtcgagcgc tacgctgatt gcatccagat
    30841 cggcgcgcgc aacatgcaga atttttcgct cctgcgccga gccgggcgat cgcggctgcc
    30901 catcctgctc aagcgcggga tttcctccac gctcgaagaa tttctgatgg ccgcagagta
    30961 cattttgtcg gaaggaaatt atcaggttgt cttgtgtgag cgcggcgtcc gtacgttcgc
    31021 cgatcacacg cggaacacgc tcgatctgag cgtggtgccg gcggtgcagc ggttgagcca
    31081 cctgcctata atcgtcgatc cgagtcacgg cacaggcagg cgcgacaaag tgcttccgat
    31141 ggcccgtgcg gccgtagcgg caggggccgc cggcctcctg gtcgaagttc ataccgaccc
    31201 tgatcacgcg ctttccgatg gttttcaatc tctttatccc ggccagttca gccagcttgc
    31261 cgaggaatgc cgggccatcg caaacctcct cgcgtcacag aaagcaaaga cggcggccga
    31321 tgcgcgggcg gctcggtagg agccggcttc caatgatcga cgccggacgc cgaggtgttg
    31381 tttgtgctct cgcagcgttg agcctgtttg cgtggagctg ccggcctcgg cggggaaccg
    31441 ctgtcgaggg ctacgccttt gtagcaacag ccgctgggaa tgcgatcacg gtcgtagatc
    31501 ttgcttcttt tgcagctatc cgccagatac cgcttggcag ccgccccatg cagatcgttt
    31561 ccgaccccgc gcggcgttcg ctctacgtca tgggagaagg cggccctgcc ggcttgacgg
    31621 aaatcgatac ggcgaggctt gtagcgaaac gcagcctgtg gcttgccgag aagccccagc
    31681 gcatccgtct cgctccgggg ggcggccggt tgtatctgct ggacgcccgg gcccaaactt
    31741 ttcagtcgct cgatctagaa aaaatgacgc gcggccgcca gatccgcctg gcgggaccgc
    31801 ccgttgattt tgatgtgtcg cccgatgggc tttgggcgtg cgtctcgctg caatggggag
    31861 aagtacaggt gctggatttg gcgcgctgga aggcgtcggc tgagattcaa acgggcggct
    31921 cgccggctgc ggtggccgtg cgctcggaca gccggcaggc gtttgtggcg aatccggata
    31981 gccgcagcat tgcggcgatc gacctgacgg ccgggcgcgt ggttgcccac cttccgctca
    32041 gcgcccgtcc tgaggttatg cgctttaagc cagatggcgg ggagctgttc gtgtctggcg
    32101 gcgatacgag cgcggtcgtg attgtctccg cctatcgcga tgagattgac cagcccatgc
    32161 tggctggcgc cgagccgcgc ggcctggccg tgaccaaaga caatcgcctg ttatttgtgg
    32221 ccaacagcgc agcgaacact gtatcggtga tcaatattga tgaccgccgc atgctggcgt
    32281 cggttcccgt cggcgaagag ccgcgtcagg tggcgctgac gccggatgac cagtatgcgc
    32341 tggtgttgaa ctacaaatcg ggcgatatgg cggttattcg cgtggcggcc gttgtcgggg
    32401 gctcgcgcga tcggattcag ccgctattca cgatgatacg cgtgggcagc cagccggtcg
    32461 atctggctgt tcaaacgcga tagcgggatc tgacggcact cctgcaagcg cctggattgc
    32521 cggtgcacaa gtcgcggcca gcccccagat ccaaggcaga agactgctgc gccggtcgaa
    32581 cactcgattg gctgtagcgg cgtgcggcgc gagaagcagg cctgctctgt tgggaagcac
    32641 tggctcggca ggtaaaacat cggttgctcg cgcgagcggg cgaggtatgt cgctgctgga
    32701 tcggtcgttc cttggttggc gccaagccgg tcttgaattt tcccgcgcgc ggcgggtggg
    32761 cttgttccgg cggggtgatc ctgaggtctc ggtccggctg tcggagcccg cggctctggc
    32821 cggctgggcg gggccgtggt aacatttggg agagcatgcc ggtgggcctg tggcgcagtt
    32881 gggagcgcgc ttccatggca tggaagaggt caagggttcg aatcccttca ggtccaccaa
    32941 acctctcagc tatccggcgc tgacgagcct ccgccggcga ggaagcaaat cccagccgaa
    33001 aggcaaacta agtacaacca ataaatatct cttcgcaccg gagccagtct gtttccggtt
    33061 acgggcgcaa cgccaataga tcgaagcgaa ccggaagggc aaaaccagac aggacctttt
    33121 ccgggacaaa cacgatccgg ctcttgaaaa atgcgccgcc gtggcgcatt ccggcggacg
    33181 cgcgtgtctt tggaattgcg cttcacgccc acgcgcgcga ttggctgatc cgagtgtgtt
    33241 agcatttcta agctcggcaa cgggcagaac ccaaccggag gtttgcatgc gcgcaatcgc
    33301 aatcagcttc gcgttcacat tgggagccgc catattggcg gcccagtcgc agtctgtcaa
    33361 gactttttcc tcggccaaag acgtcgaggc gctcatcgcc aaggcaaaga gcgagcgaaa
    33421 ggacaaccag cccgtagtca ccgaacgcat ccttcagctt ccgccctaca atgcgaatct
    33481 cgaataccgc gccgcggtcg ggcccgcgtc catccatgaa cgggaagccg agatcttcta
    33541 tgtcatcgac ggctcagcga cgatggtaac gggcggaaag ctcgtggatg agtctcgaac
    33601 gaatccgaat aacctcaccg gcaagggcat tgaaggcggc tcgtctcggg aaatcggaaa
    33661 gggagatttt atcgtcgtac cggaaaaaac gccgcactgg ttcagcgcga tccacgggac
    33721 cctcgttttg atgtcgctgc acgtgccgcg gccggcggct cactaaccgt tgttctagta
    33781 attggcaaca cgagtgcgtc agggcgagcg cggcgggatc cggctggcgc cggagcgttt
    33841 ggtccgcgcg agccggctgg cgggcaggga tcaccggcgg ctggccgccg atcgatgtcg
    33901 cgcccggcgc gccgctagac cggctcgggc tttccgcagg cgtggacgcc gggccgccgg
    33961 agttcgggat tgcggaggct acggctcgat ccgctccgga gatggccggt tttcccgaat
    34021 caagtagtag gaaccgccgg cagagacgtc tactaccacc tgccggtaac cgggaatcgt
    34081 gatcccaaca ttccggcggc tgcccatggg cgcatgagcg taaagcagcc actcccggcg
    34141 aggtgcgcgg ccgaggacca gcgacagcga aaaaaccggc agcggcgtgg cagcgttata
    34201 gggccgcggc gggtccaggg ttgtgtccag caggaaccaa cggtcctccg cggcgtaccg
    34261 ctcgacagga cgcgactggt acgggtgctt ccaggcgcgg ttcggcgcca gttcgccgcg
    34321 ccgccagaat cgccgcagcg cgccgttacg gtgaatgctg ccgaccgcct cgaggatcgc
    34381 gtcgagccac ggccccgctt tctccgcgct atcagtccag ccgcgaaact cccgtacaca
    34441 ccgcggacgc agcagccaca ggccgaactg caccattgcg gcgtagcggg ccggcgtgaa
    34501 gggcgccacc ttggcaagct gatcgcgttt ttcctgctcg ccatcccagg ttgaaaaccc
    34561 gaaccagaat cgcgggttca gccgcatggc ttcccgcaac ataaagaccc aattcaaagc
    34621 ctcgccttgc gggctccaga tgttgtagtc ggtgctggcg ttccaattgt gcagatagaa
    34681 cgaaggagag ccgccgtccc agatgagagg agcggggtcg atgaagcggc ctgtgggcaa
    34741 ggcgtagtcg tcccattgct cccagcgggc gtagtgcggc ggtccgaaag cgtcgtagcc
    34801 gatgaaaatc gagtgggatc gccaggcagg cgcaatgagg ccgccgcgca tggcctgtaa
    34861 caaggcccgg tagcgttcaa tccagccttg cgccacgacc gagcgtttga agtcgccatc
    34921 atgagcgggg ccgtaagcat cgagaaaacg cttcgactgc tcgatggcgg tccaggcaat
    34981 ccgggactct tcgttgttgg atacgaagac caccaaaggc gggtcaggat accagcgctc
    35041 gatttgttgc agcagcgaag aggaaaccag gcggcgcccg gcatcgatcc acggctccac
    35101 gggtcccatc ggatcgagct gctttcggat cgcgccgtcc ctttggatga cgttgggatt
    35161 ctgcgatgcc ggcaagaggt aatacggagg ttcatataga atcgaggcaa agttcgtccc
    35221 aaccagagaa atcgggagct tgagctctgc ggcgcgttta atggccgctt tgtaataggc
    35281 gagcgcggcg ggcgtcaggg cggcgtccgg gcctgggagc gcaaacgaga gcagcacgta
    35341 gccacctgat tcgatctttt cgagctgata gttgggggaa aaggcatggg tattccaatg
    35401 ggccgccaga ggcaaggggc ggccggcagc ggcgttatta gcacgcgtgg cttcttcgcg
    35461 gatcgctcgg gcggtacttg agagcgcccc gcccgacagc ggcgcattca cggccgccaa
    35521 gcaaagcaaa acagcggttg cttgtgttac gcattttcga atctggaaga ggacggcaaa
    35581 ggttgttgca gcgcgccggg cgccagcggc ctgtcgagag aaggtcactg ctccaatgta
    35641 taggccagcc ggggggcgcg ctctcgctgc ttctccgccg ccacgcgttg gtcattcagc
    35701 agcttgtgat ggatttcgcg ctcctgagcg gcgcggtcgc ggtcgccctt ccgatcgtag
    35761 tattgtgcca gggcgtagtg agccggcgca tagctgccgt tgaggtcgat ggttttttgc
    35821 agggctgctg ccgcttcgtc ccatttgccc tgacctttca ggagcttgtg caactcgaag
    35881 tacgccggcc agaaaccagg agcggcctcg atcgccttgc ggaacaacgc ctccgactcc
    35941 tcgggaacga ccagccccag caggtagggg ccgatcgggc tggacggttt ccgctcgcaa
    36001 aagcgccgga gccgggagac cacctcaggc aaacgcggct gcgcataggg aagcagaacc
    36061 tcgagtgacg cgtaggcttc ctccagatcg ggatcggcgt cgatcgcttt caaaaagacg
    36121 accagcgcct cgtcgtattt tccgagggcg taaagagcca ggccaaaaga tcgcagaaca
    36181 ttcacgtttg ctggaaagcg ctgggaggcg tttcgcagaa cgatttcggc gggctcgggt
    36241 gtgtcgcgat cgagaaataa ttgcgcgagg tcgaggtaat aaggcgcccg ctcgggatcc
    36301 cggcgaatcg cctcctgaaa ttcctttacc gccgtggccg catcgttgtg agccgccgct
    36361 gccttgcccg cggtgttgtg tagcagggcc agagctcgcc agatcaacgg agacttggga
    36421 ttcagtttgg ccgcgaggtc gagatgccgg cgggcttcgt ccaatttgcc gtgcttgagc
    36481 agcaccaggc cgaggcggta ttgcaaatcc gcgtctcggg ggtgcgcctt ttcgagcgca
    36541 gtgagcatgg cttccgcctc gttcaatttg ccggcggtag cgagctgcac ggcgcgatcg
    36601 acgctcggct ggtcggcggc gaaagcagcg gcagcgagca acaggagcac gaatttcatg
    36661 gcgcagccaa cttgcactca ttctactgca atgcccgccg cgggcgtcgg ggagacggga
    36721 ggcgcgcgga gatagaagaa cgaggtctga ttcacgattc gaggagagag cattctctct
    36781 attcgagggg aaggtcttct tgtttctctt gccgggctgt gctaaagtcc ctctgaacta
    36841 tttttccggc tcgccgagtc ttgcgaatca tgaaggggct gatgtggtga atcggaggca
    36901 atttctaacc tccgcaacgc tgcttccggc ggccctggga gcccaacagg aaggcccgcc
    36961 tggcccggcc gtggaagatc ctgtagaaaa aataaccccg ggagagaagc ccacagttgc
    37021 ggtttgccac gtcggctttc gcccgcgcgt cggccgtaaa acgcttgtgg tgcgcgcggc
    37081 ggccggcgcg ccggccaagg aattgatgac cggcgtgggc gctcggaatc caatgccttt
    37141 tttgccggga ggcgcctcga tgcttcgtca tgggatagtc attgcgtgtc tgcttgcttg
    37201 tgcgaccttg ctgcaagggc agacgtccac aggtaccata caaggccaag tgcgggatgc
    37261 cagcggagcg gttgtccctg acgcccgtgt gacgatcact aatctccgga cgggggtgct
    37321 caacgaactg cgcaccaatg gcgaaggccg gtatgtcatg ccgttcctgc tgccgggaga
    37381 ttacgccatt gccgtcgaaa agccgggttt ccaacggttc agccggagcg gtatcaaact
    37441 ggatgtcggc caaaacgaat ccgtggatgt cacgctggcg gtaggcgatg tggccacgac
    37501 tgtccaagta gaagggactc cgccgccgct cgccaccaac agctcgactc tggccacggt
    37561 catcgagaac aaacggattg cggacttgcc gctgaacggc cgcaatccat tcgatctggc
    37621 tgccctgtcg cctggcgtga ttcccatggc tcccggaggc ctcggcgccg gaatgtcccc
    37681 cacaccctcc atcagcggag gccgcgacgc caatagcgag atcagtatag acggaacttc
    37741 tattgttctt ccggaaaaca acgtttcgat cactacaatg gcgttcacgc cgtcggtaga
    37801 tgcgatccag gaattctcgg taatgaccaa tactctttcg gcggagtatg ggcgttcggg
    37861 cggcggcgtc attaacgtgg cgaccaagca gggcgccaac cagcttcatg gctcggcttt
    37921 tgagtttttt cgcaacagcg agctcgatgc gaatgacttt ttcagcaacc gcgccggaat
    37981 cccgcggcac gcattccagc ggaatcagtt cggcggcaca atcggaggtc ctgtggaagt
    38041 gcccggggtc tatcgtgggc gcaaccgtac tttctttttc ttcgatgaac agagtacccg
    38101 ccagcgaagt ttcagccagt ttacgaatac catgccgctg gatgcgtgga gaaacggcga
    38161 tttctcggga ctcaggaact cagcgggcgc gccgctgctg atctacgacc ctctgacgac
    38221 ccggcccgac ggacagggcg gctatacgcg ggatcccttc ccggggaatc agattccttc
    38281 cgcgcgcatc gatcccgtag tgcggaagac cgaggcttat ttcccggagc ccaacacaac
    38341 gcccatcaat cccttcacgc aggtgaataa ttacatcatg gtgggcaaat cgcctacaaa
    38401 tgatgatcga tttgatgtgc gcctcgacca caatatttcc gaccgttggc ggatattcgg
    38461 acgtttttca caccaattca acaaagtgac gcccgtgaac ttcttcaata acgtggccac
    38521 gccctctggc aatggcccgg agtccttcag cttttacagc gcctcgctcg accaaactta
    38581 cacgctgaac ccgactacaa ttcttaacct gcgctacgga tttggccgtt tctttgacgt
    38641 gcggcgccct ttctcggccg gctttgatat gacgactctc ggtttcccgc aagccgttcg
    38701 ggatcaggcc gctaccgagc ttttggcttt tccccggttt gacattaacg gaatttcttc
    38761 catcggcgct tccggcttca cgaatgtgcg gatcgttccc aatagtcaca tcctccacgg
    38821 ggacttgacc agagtgctct cccgccacac tttgaagctg ggcgtggaat accggaagct
    38881 tttggaaaac ttcctgcaag acgccgctcc ggatggcacc tatagcttca accagcaatg
    38941 gacccagcgg gatccggtga cgccgagttc gacggccggc ttcggtttcg cctcttttct
    39001 cctgggaatt ccttcgagcg gcagcatttc gcatgaaccg gccaccgcca ccgcgagcag
    39061 ttattgggct gcttacatcc aagatgattt ccacgctacc agcaatctga ctttgaatct
    39121 cggcttgcgt tatgaagtgg acgttccccg cacggagcgg tacaaccgcc taagctactt
    39181 caatctgaac gagccctcgc cgatagccgg cagagtgccc ggcttcccga atctggttgg
    39241 ggcgatgcac ttcgtgacgc ccggtcaccg ccagcaaacc ccaaccgact ggaataattt
    39301 cgggccccgg ttcggctttg cgtacaagat cggatccaag accgtcgccc gaggcggcta
    39361 tgggatcatg tacgctccct cagtcatgca ggcggccggc tacaccggaa ctatggggac
    39421 tgaagggatt acctcctcca ccaatttcat cacctcgctg gatgggagaa ttccgatcaa
    39481 tttcgtaagc aatccctttc ccaacggatt caatttgccc ctcggggcga cacccggacc
    39541 gaccagcggc ccctctactg acttgggatt gggagccgga tcgagctggt ttatcgacta
    39601 ccgcaacgcg ctcatccagc agtggaattt taatgtgcag agggatcttt tttccaacac
    39661 cgttatagaa ctgggctacg ttgcgagcaa gggcaatcac ctcgtcgacg gcgaaggcgg
    39721 catggcttac aaccagctcc cctcctcgtt cttttccctg ggcaactcgc tcaacaatct
    39781 ggtacccaac ccgttttacg ggattattgt caatccgacc tcggttctga gccagcctac
    39841 ggtcacgcta gcacaattgc tgcgccccta tccccaatac acgtccttga gccccagccg
    39901 caagccgcag gccaactcgc tctatcattc ttttacattg cgcgcggaga agcggtattc
    39961 ccagggtctc ggcttcctgc tcagctatac attcggcaag ctgatcgacg acgcttcctc
    40021 caccgtcacc tttctcgggc cctccggcaa taagcaggat ttctacaatc gcaaagccga
    40081 gcgagctgtt tccactcagg acgtctcgag ccgattggtt atcagcacgg tgtatgactt
    40141 gcccctcggc agagggcgca agttcatgaa caacgcgggt gagccctggc agtttatttt
    40201 cgggggctgg cagactaacg gcatattgac tttccaaacg ggaactccgg tgattctcac
    40261 gcagacgcaa aacaatacgg cgctcggatc gcccggccaa aggcccaaca acaacggcca
    40321 gtcggcacat gtgagcggcg gctccaagga ctcccggctg aaacagtggt tcaacacgtc
    40381 ggtgttttcc ttcgcgccac cattcacttt cggcaatgtc ggccggacct tgccggacgt
    40441 gcgcaaccca gggcaacgca acgccgacct gtcgctgttc aaaaacttcc ggttccggga
    40501 agaccgcttt acgctgcaat tccgcgcaga ggcgttcaac gccatcaata cggcccaatt
    40561 cggtcctccc ggcgctcaag tcggttcgtc gaacctcggc gtcatcagca gctccggcgt
    40621 ctcggcgcgc caaatgcaat tggcgctgaa gctgctgttc tgatttcaag cggaaccttc
    40681 ctcggcttca tgggccggtg tttccggggg caggaatccg gccccttttt ttcggccacg
    40741 gccagcgaag cgagggctag ttggcgtttt ttgcccactt ctgcacgcgc cagttcttga
    40801 tctccgatac atagatgctg ccgtcgtttt gatccaccgc aatgctgtgt gcgtaatcga
    40861 gtttccctgg gatcttcccg taggagccga gagtacccag gatctggccc tccgtgttca
    40921 gcttgacaat gcggttgttc aggccatcgc acatgtagag agcatcttcc ttaggaacgt
    40981 aatagagacc ccagggctgt ccgacgtccg tccatttccc aagaaacctg ccgttctgat
    41041 cgaagatctg gacgcggctg ttgcttcggt cggcgacgta gacgcgcccg cgcgaatcga
    41101 gggcgacgtc gtgcacgagg ttgaactccc cgtcgccaac gcccttcttc ccccagtgca
    41161 gcagatattc gccgtcttta ttgaatttaa tgacccggga gttgccgtag ccgtcggaga
    41221 tgaagaagtc gccgtttgga gcaaacgcca cattggtggg cagattgaag tactccttgc
    41281 tgtcattgtc gcctttgatt ccctgccggc ccagcagcat gagaatcttg ccggcgggcg
    41341 tggttttgaa aaccacgtgg ccgtcccggt caatgaacca aagattgccg tcgggatcga
    41401 cccgcgcccc gtgggctccc ttcacgcgca gcacattctc gttccaggcc tgaattactt
    41461 cgccgtgctt atccagctcg acgatcgggt gatcgctgcg gttgaatacc caaacgttgc
    41521 cgttcctgtc tacatcgacg ccggaagctt cgccgaaatt cattcccttg ggcagcttcg
    41581 cccaatccct caccaggtga taaggcagcg gagggccttg cttcagttcc gcggcggggg
    41641 ccggcgcgag cgaaaggagg acagttgcca gagcaaagag gaggattttc atggcgctca
    41701 gcatagtgcg cccgttccac gatgtcaacc ggagatcggg agccgtgacg gagcgggccg
    41761 ccggagcttg gggagcctgc taacagcgca aaacctcgcc ggaatcgagc ccaaaggtct
    41821 cgggctcttt ttttggcgtg cggcgcccgt cataggaagc tacgggcgtc cgggactcct
    41881 ggagtgcagc aagcctgagg cgggctacct cgccgcaacc acgcgcaggg gtttatccaa
    41941 cctgaatgtc aggacagttt cggccggcac gcggaccgcg ctgcccttgg tcagaatctg
    42001 cgtcagagcg ccggctccgg cgccggaagc cgctccgatc gccgctcctc tacctcctcc
    42061 ggcgatagcg ccgataatcg ccccgagcgc cgcgcccccg ccggtgtact ccgcggtgcg
    42121 tttattcgcg ccaatgcctt ccttgcctcg ctgctggacg tcggccgtgt ccagccggta
    42181 ctgccggccg tccacggaaa cagtttgcag atcgagcgcg aggtccgacg ccccgcggaa
    42241 ccggccaccc ttcgacgcgg accggatgac aatttgcgca ttggatccgc gcgggatcac
    42301 cacatccccg ttggcatcca gaacatcgct agtgacctcg gccgcaaacg tctggccttc
    42361 caccgccttg ctcgaatcaa tcgtctcctc ggttcgcacc gacacctgtg tgcctactgg
    42421 aagcgcgtag gtcttggtct gcacagcgct ctgctggggg tggtaatggc cttgatgaac
    42481 cggctgctgc gactggaccg gtggttgcgg ctgtcttgcc ggcggggccg attgtgaagg
    42541 cgccgccgga ggagcctccg cctgggcaac gggctgctgg tcatagttca ccgattgaac
    42601 gtccttcata gcaatcgttc gggtcgtgtt gtcatcgccg gcaagagtga tttgcgaggg
    42661 cgaactcgcg gtgacaatgc cactgacctg ggttccgtca cgcatcaata cggtggcgtg
    42721 cagccgctcg gaaggcggcg ggccggtctt gcaggatgcc agcgccaggc aaatcacgat
    42781 tgtgggcaaa ataataagtc gccgcataga aatcactcac ccttcctgga gcccagtata
    42841 gcgaaaccgc gcgtgcatgt ttgaatcctc cggcaactcg cggcgcgtgg atattctctc
    42901 atcattgtcc gcctgacggc atagaagagc ccggacgagc ggtggtagag tagccctatg
    42961 tggaaaacca ttgcgcaggc cctccggctc ggagttcccg agcagcagtt ggacgccatc
    43021 ggcccgatac tcgattctct atggaatgac acgcgcaggg ttcttgaccg cgacctgagc
    43081 ctgacggatc ctgcaatcac attccaagcc ggcctcgggg gcgagctgtg accattgcgg
    43141 aactcggagc gcagatccaa tcgggaaaga tttcgccggc cgagctggcg gcggagacgt
    43201 ttacgcagat ccgcaaactg aatccggtgc tcaacgcgta tatcaccgtg atggaatcct
    43261 ccgctatgga ccaggcgcgg caagccgaac gcgagatccg ggcgggaaat taccgagggc
    43321 cgctgcacgg cattccgtac gcggccaagg acctgtttta caccaagggt acggcgacta
    43381 cggtgggatc gaagattttc caaaatttca cgcctgatta cgacgcggcg gtgatcgagc
    43441 ggctccgcga tgccggcgca atccttttag ggaaggcggg cctccacgaa ctggcctatg
    43501 ggatcaccag caacaacccg catttcggaa cgattcacaa cccttgggat ctgtcgcgca
    43561 ttccgggagg gtcgagcggc ggctcaaccg cggctctcgc cgccggaatg tgcgcgtttt
    43621 ccctcggttc ggatacagga ggctcggttc gcatccccgc ggccttttgc ggcgttgtcg
    43681 gccttaagcc cacatttggc tgcatcagcc gtcatggcgt gtttcctttg ggccacacgt
    43741 tggatcacgt cggccacttt gcctggacgg tccgcgacgc agcgatcgtg ttgaatgcta
    43801 tcgccggcca tgatcctcgc gacggcagtt cggcagtcag ggctttccgc ccgattagcc
    43861 tgccggcgga cgtgaaactg cgggggttgc cgatcgggat tccgcggaac tattattttg
    43921 aacgcctgca accggaagta gaaaacgctg tgcgggcggc ggtgaagacg gtggaggatc
    43981 ttggagccga aatccgggaa atcgagatcc cggaaatcga ggacttcacc gtcatcggcc
    44041 ggctcatact gatgcctgaa gcgactgctg tgcaccgccg ccttctccag acgcgccgtg
    44101 ctgatatcgg agccgatgtt ttggcattgc tcgatgaagg ccagttcatc ctggccgccg
    44161 attatctcga cgcacagcgc cggcggcggc aattgattca aacctttcag cgcatcttcg
    44221 agcaggtgcg cgccattgtg acgccagcga cacctttgac ggctcccaag atcggccaga
    44281 gcactatcga gatcggcggc tgccaggagg acgttcggct cggctcaacc cgcctcgtgc
    44341 gagcgcttaa cgtggctggc gttccggcgc tttctgttcc ctgcggcttc gatgcgcagg
    44401 gcctgcctat cgggttgcag attttcgggc gcgccttcga tgaagccaat gtgctgctgg
    44461 tcggccacgc ctatgagcag gcgacggaat ggcacaagcg ccggcccgaa atcgccgttg
    44521 cgccggctgt agtccgctag ggatcgcggg gctttagggc ttctcctccg gcaattcgcg
    44581 ccttagtgag attcagaatc gggtatacac ccgattctgc ttcgtttccc gccacaatag
    44641 aatggccatt gccggttcag gagcgagatt ggcgctccgc cgcgggatag cgatccggcc
    44701 gcgcggatat tctgaagccg gcgtccattt cttgattctc atggcagaac cactccagac
    44761 gtccggggtc atggccgcca gcccccgcgg gcaggccttc ccgaaagccg cgagcgcccg
    44821 catcggcgtc gccatttctg cgcagagacg ccctcctctg ctggtgctcg gagctttttt
    44881 ggccgtctgg tctgtgtggt cgttccggat ccacgcgtcg gctgccttca gccaatatca
    44941 cgacgacacc atgctgatgg tttcggctca ggcgctcgcc gaaaacgggc attacacgat
    45001 tccgagtctc gccgttccca cgagcgcatc gccgccgcaa accaaatatc caatttttgt
    45061 tccatggctg ttctcgctcg tctggcgctg gaatccgcac tttccggcca atctggaagc
    45121 gatgcaagcg ctggccgctt tctttggcgt cctggcctta gccgccgcct atgccgtgct
    45181 gcgaactttg aaagtcggcg cgtgggcagc ggtgggcatg acgatatttc ttgccttgca
    45241 gccggtattc gtggtgctga gcaccaagct gctgacggac gtgcctatga tggcgctcac
    45301 gctggccgcg gccgcggctg ctgaacgcgc cctcgggaag cgccaaggct ttttcctatg
    45361 cgcggctgcc ggatttctgg ccgccctggc cgttctaacc cgcacgatcg gagttgccat
    45421 cgtgggcggc tttttgattg ccgccgttct ccggcggcgc tttcgcatgg ctgccgtatt
    45481 tatcgcatgc tgcgcgccgg ttgtagtttg gggactttcc cgcgcgcacg acagccatgt
    45541 gctcgatcca tgggtgcgga gcggcggggc cgcttttcaa agcaactggc tgttttatac
    45601 gaactacatc ggcttctgga aactcagcgt ttcctcgctg cacgccttcg gctatctgat
    45661 cgctcaaaat ttcctgatgt ttgccataac ccccggcttg tattttcaat ttccgcctcc
    45721 cggcggcctg ctcatttacc tgctgtttcc gctgacggct gctattctgg ccggagtctt
    45781 gcggcacgcg cggcaaaacg gatggcaggt cattcatttc gtctttctct tgcatacagc
    45841 gatcattctc tgctggacga accgcgaaat gatcgagcgg ttcctgctgc cgtttttgcc
    45901 gttgctgtgc gccggccttt ggacggaggg gcgttatctg agcgcactgt ttgtgcaaag
    45961 cttccgggcc ggacgggcgc gggcaaggtt tctgatggcg ccgctggcgg caggcgttgt
    46021 cttgttcgcc gcccgcggtt tggaggccgg agcccgttac gactgggact acgtgacaga
    46081 ggtgatcccc agccatgccc gccggttgca acagaaacgc gaactttacg cgtgggtaaa
    46141 cgccaacacg gcgccttctg caacctttgc cgcctgggat gacggaggcc tctatctcta
    46201 cacccgccgg cgcggcatgc gcccgattgc tttctccacc gagtattact acagcaggaa
    46261 tccgcagact ttgacggccg aaatggctca tattttcgac acggcccgcc atattcacgc
    46321 ccagtattgg gttcgagtgg acggagaata cgccgatacg caaattgaga aagagataga
    46381 aaaagtcctg gcgccgctgc cggtcctgtt tcgcagctct tcgggagctg tccgcttgta
    46441 tgatctgcgg cctgtcgtga cttcccccgc gtctcaggaa gaggcttctg cgccgcgctt
    46501 atgaaaatcc ggccgttata cgggaggggc gcaaaatttc ttccttggct gattttgggt
    46561 gcgattctgg cggccttcct cggatacacc cgctcactac atccagaaaa caatttcggc
    46621 agatatcacg acgacacggt ctatttcagc gccgcccagg ccctggccgc aggacagggc
    46681 tacacactgc ccagtttgcc ggttcctggc gggaccgtgg cgccccagag caagtatcct
    46741 tccttgtacc cgtggctcct ttcctgggta tggcgatgga agcccctatt tcctcaaaat
    46801 gtgacgctgg cgctccggtt gactgtattc tttgcctcct ggttcctcat tgcctctttt
    46861 tactggctgc ggagttggct cggcgagtgc gccgctttaa tctgtgtagc gctaacggcc
    46921 ttgcatcctc atttcctcgc cctgagcggc gccgttctca gtgaagtgcc cttcatggcg
    46981 ctggcgatga cggccatcgc atggaatgac ctcgccccgg aaaacgaaag aacctgggct
    47041 ccgctgggga ccggagtcct ggccggggca gcgaccggga tccgcagcat gggagcggcc
    47101 gtcgtgcttg gcctggcggc cgcagccgtt tcccgcaagc ggtatcgcga tgcagttctg
    47161 atttgtctgg ctgcggcccc ggttgtcgcc gccacgcttc ccgccggcgc cgtcgccctg
    47221 ccgggcggtc ccgcagccag cgccggttgg cggcaaacct ggctctatta cacaagttac
    47281 tgggcgttct ggaagctgag cgttcctacc tggccggttt ttctggagat ggtgcaatcc
    47341 aatgtgaaag acctgctggt gacgccagcc tctcagtgcc tgtttcccgc gccggccgcg
    47401 ctggggcctt ttggcggcgc gttgctctgc atcctgctga ccgcgggaat caccgccggc
    47461 attatccgaa aagcacgccg gcagggctgg caaagcattc actgggtgct gcttttctat
    47521 ctccctgtta cgctggtgtg gaattacccg atcatgaacc gttttctggt tcccttcttg
    47581 cccctgttct acgccggcgc atggatggaa ggcgcccatt tggcgggcat ggttcggaaa
    47641 acgtttggcc aacgcggtgc gctcaccgaa aaaatgattg caggactcat cgccgcagga
    47701 ctggcgggga ccgcttccgc ggctgccgcc acttacatga acgattcgcg tgcgcggttg
    47761 cgagaggacg ggaaacagcg cgccgcgctg caaacacaga aagccgaagc ataccactgg
    47821 atcacggtcc acaccgatgc atcagcgaag atccttgctt ccgaggacat ctgcctctat
    47881 ttttacaccg gccggcaagc cgtgtggcca attgctttca ctaccgcgcc attttatgag
    47941 acagatactc gcgatctaca attgcagctc aaccactgga ccgatgtggg ccggacggtc
    48001 ggggcgcgct actggcttgc cgccgatggc gactactata tcgaagcggt ctcaccgctg
    48061 gttagagata agatggcggc ttcgcaggcg gcgctgccgg aagtgttccg aagcagtgcg
    48121 ggcgcggtcc gtatttatga cctgtcaagc tggcaaactg agttcagcgg acagcgaatt
    48181 tcttggtcgc gcccggctgt gaaatagaat tgaatctgaa atttcgctga aatcttcata
    48241 aactcgcatc gttttctcaa ggtcgtgtat ccggttttga gaacatgcaa gacgagagca
    48301 ggaactggga ttgaaagtgg tattaccgga ctttcctcgc tcctggcact cgcagaactc
    48361 tggaggctgg cgcgcggtgg cgcgcccggt ggcgctgcat cgatggtttt agaaggtgct
    48421 cgggggaaac gcttagattt cggaaggagg tttcagctgc cgccgcggtt tgattttcct
    48481 taggcgccca taaggtttct cggcagcggg gcgggatttt gcagacaagt cttttccatt
    48541 caataacttc agggaaaagc ctcggtggga tcttcctgcc agtactattt ggatttaaga
    48601 aaattctggt tgcatcctag gaaaaatctg atatagtacc gaactgtccc tggtactcat
    48661 agatggctta tgccaacagt ctacagtgat aagccatgac gccaggacag gttggggagc
    48721 gccgggagag aatcgagatg attactatag ccacttgtga atcgcagccg atcgtcgtgg
    48781 aaggattgcg ccgcagtctg gaagggacaa aagatctcca gttcgtcggt tccgtggcga
    48841 cggcgaaagc cgcgattgag ctggtcgagc agcaaaagcc ttctgtgtgc ctgatcgaca
    48901 aggccttcgg cacgaaggct gtcttccagc tcatcgccga catgaaggtt cgctcaccgc
    48961 agaccgagcc gattttgtgg gcggccgaga tcagcgagat cgaatcgttc cgggcgctcc
    49021 aggtcggcgc tcgcgggatc ttgaagaaga cgctcccggt cgcgacgatc ctcgattgcc
    49081 tgcggaccgt cgctcgcggc aacatctgga ttgaaaattc catctccgac caattcgtcg
    49141 gcttcatcaa caggcgcaac acgccccacc tgacggcccg cgaagaagaa attgtggcgc
    49201 ttgtgacgcg ggggatgaaa aacaagcaaa tcgccgacgc cctttgcatc accacgggca
    49261 cggtaaaagt tcatctcatg cacatcttcg agaaaacggg cgtgaaagac cgcttcgagc
    49321 tggccatgta cggccgccgc atggacgccg gctcgcttga gctcgaagag aaccaggcgc
    49381 tggcggccgg ctttggcggg tagcgccggc cgctcttagt tggctgcggc aggctcgtag
    49441 atgtagtcgc cggccagcag cgatccggga atgtgttcga cgagccgata gtttcggcgg
    49501 atcgcatcca gagccggggc aggaagcgaa ggcaactcgg cattgcgggg aacggcggcg
    49561 gggtggagca ggtcctcaaa caaaatcacc aaggaaaact ttccggctgc caaatcgcgg
    49621 caaaccggcc cggggtccac tcgccccgct ccgaccatca gggtgtagat cagaggctcg
    49681 acttcaagcc gccgccccga ctgcaccaga ggatcgaatt gcaccgagat caccgggcct
    49741 ttcgccgaat ccaaataagg ccggagttgc gcgtattcct gtcggcggcg catctcttga
    49801 gaaatgcggt cgctcagaat tcttccactc atcacgaggt tcagcgctat atagacgagc
    49861 agaggagcct gcaaaagcgt cacggagctg cgggactttt caaagcataa aggaaaaaag
    49921 cggagccggt gcaggctcca cccggcgcac aacgccagag ccgtcatcgt ctcgagctgg
    49981 tagttgaggt ccgcgccgat tttggatccg gtgctcaaca aaacggccat cgatgccccg
    50041 gcgtagatgt agagcgggtg gaggccttcg cgtatgcttg ccggcaggcc caccaatgcg
    50101 atgagcatcg atccgcaagc caccggcgcc agatagcgta actgcgcggc gaacttaacg
    50161 aaactgaacg ggttgatgtt ggcgaacacc gtgttttcta gaaatccgcc ggaggttccg
    50221 tagttcacgc cggccgccaa agaaattcct acagcggcaa tacaaacggc aaaccgcacg
    50281 gcgcgccgcc gattctcgaa ggcgaggcac acgaaaatag tgaagccggc ggcgagtagg
    50341 gtctgtttcg tgaacagggc cgccaacacg aaagctcccg gcaacagcag aaacctggtt
    50401 tcctgcgccc ggcggtagcg caaaaagaaa tagagagctg tgatcgaaaa gcaaagtccc
    50461 agcacgtcga cctggccgac ggttccccaa aacatcaaat tcgccgtgga agcggccaac
    50521 agcgtccccg cccaggttgc atgcgtttcc ccagtgcaca atcgcaaaat tttccagacg
    50581 ccccaaagta tgccggtcag tcccagaatc gacaagagcc gccctgcttg aaaggggggc
    50641 agcccgagcc ggtgaagagc ggcctgcgcg gcataaaaaa gcggcatgta cggggacacc
    50701 gtgtatgggt aggatcctag tttgtggtat aggccctggc ccgaagcgag acccagggaa
    50761 tggatggcca cggcggattc gttttgggtg acttcgtgct gcgcccatag cgcggcgcca
    50821 atgtgcgcgg caatcagcgc gagcgcgccc gtcgacagta cggcgagcgt ccggcgaaca
    50881 tatctttcca tggcgcgccg gcttccggag gccgcttggg cttgggtgga ggtcacagaa
    50941 tgcttaccca gcaaaaagaa tcggctgcca gtatagggga acccgcgccc gcacaaatca
    51001 ggacgcccct ttattttcga aattcttttt ccttagtcca cggaccgcct cagcaaaatc
    51061 gcggcgtttt gatatagttc cgaataacga tgcggcaaac cggcaggtcg cgcgcaattg
    51121 tcgccctcat tctactggcg ttcctcgcag agctcggggc gcgccgtccg caggattggt
    51181 ttggctggta tcacgacgac acgatttatt tcagcgccgc gcggtcgctc gccgagggcc
    51241 gtggctaccg gctgccgagc gtgccgggaa cgcctctgga aaccaagtat cccgtgctct
    51301 acccctggct gttgtcctgg atctggagat ggcggccttc ttttcccgca aacgtctctt
    51361 atgcaattgg cctcacagcg ctgttcgcgt gctggttcct gattgccagt tttcaactgc
    51421 tccgcagcct cggttttggg aacgccggcg ctgcttgtat tgtggctctt tgcgctttcc
    51481 agcccagctt cctgtttctt agtggcgccg tactcagcga cgtaccgttt atggctctgg
    51541 cgataacagc cgcagtgatg gcggattccg ctatgcgtcc cggaacccgg atgacacgcg
    51601 cggccgcggc cgccgccgtg acgggattgg ccgggggaat gcgagggttc gggatggctc
    51661 tcgccgccgg aatagcgatt ttcgctattt atcggcgggc gtttcgccag ggcgccgttt
    51721 tctgcgcgat cgcagcgccg ctgctcgttc tgagcctctc ttggccccat gcgaactcga
    51781 gcctggaagc cgccgcggcg ggcgacccgg gttggcggca gacctggctc ttttacacca
    51841 gctacggaaa attttggaag ctctgcgttc ctaccgccgg cgtcctgttc gcaatgcttg
    51901 gggaaaatgt gcacgctttc ctgcaagcgc cggcgtcgct ttgtttgttt cctccgctag
    51961 gagaaggctc gtatgcagga actctgttgt gcgtaacttt gagcctggga attctggctg
    52021 gcgccgtccg gcaagcccgc cgctcggaat ggaaacccat ccacttcatc tttgttttct
    52081 actcgtgcct cgccctgatc tggaactata cactgatgga ccggtttttg ttgatcttcg
    52141 cgccgctctt ctatagcggc ctgtgggccg agacggagca cttggtttcg atgctgcgcg
    52201 acaacttcca gccacgacgc ccgttcagtg aaaaagccct ggcgggggtg ttgggcctcg
    52261 cgctggccgc tgtgggagcg cgagccgccg tagaatatct tcatcacttc cagccggatt
    52321 cttccgagct gaaagccaaa cgggccgctc tcgcccggga aaaatcggag acgtatgaat
    52381 ggatccgcaa aaatacgggg cctgcggaac gcttcgtcgc ctatcaggat gtcagcttgt
    52441 acctcaacac caaccgccag gcattgcgtc ccatcgtctt ctcgacacag gcgttctaca
    52501 aaaaggatga ccaggtcttg cggcgcgacc tggctcatat caccgatgtt gcgcggcaca
    52561 tcggagctcg atattggctg gcgagtgacg acgattttca gttggagacc ggcctacctc
    52621 tgatcgaaaa gagaatggcc gagatcaagg ccgtcctgcc cgtagttttc cacagccgaa
    52681 cgagcaaggt gcaggtgctt gatctctcct gcctgcttca cgcggaacag cgggcgtgtc
    52741 aggtaatagt gcccgtcctg tgtcctacga ccacgggctc ttgaaaactg gggtaccgca
    52801 aagggttaaa ccggagtacc aggcgcgctg gccgaggctt gttgtggacc gccgcgtaga
    52861 gctggtaagc gcctgattac aggaagaatc gccgcactta tcacttaagg catactactg
    52921 aatggcgctt gtttatacca ccgacgaacc gatatgtagt atgtcgaaaa ttctaaaacc
    52981 tataacgaca gtcgtaccaa aatttatact cttgttcaat tgttttctat acgtactagc
    53041 tactattctg ctttctcgcg aactgaaaaa acggtcctgt tggatacgaa agccgccgga
    53101 tagaaaaacc tctctgggac ccggcggagc tagattcgat gagcgaaact cgcccgggtc
    53161 aaaattcgcg gtggatccct tcgctaagcg agatggtttt cgtctcggtg atggcgtggc
    53221 tcttcctcgg cgcttcaggg ccggaagcgc tgctcaccga tggcgacacc ggctggcata
    53281 ttcgcactgg cgattatata ctcgccacgc acagcttccc caaacaagat ctcttttcgt
    53341 tctctaagcc ggaccagccc tggtttgcgt gggaatggct cagcgacgta attttcgccg
    53401 ggttgcacaa attcggcggt ttggctggcg tcgtactgct tgcgggagtg ctgattgccg
    53461 ccacttcggc cgtgatgctc cgctacatgg tgtggcagcg atctgacttg tttgtctcca
    53521 tcgtggtcat gctggtggcc agttccgctt cgacggttca ctggctggcc cggccgcacc
    53581 tgttcagtta tttcttcctc gctattgcaa tctggctgct tgaggccgac cggcagcgtc
    53641 cgactcgttg ggtattcgcc ttagtcgggt tgtgcctggc gtggaccaac ctgcatggcg
    53701 gatttctggc gctgctttcg actctggcca tatatctgct cgggagcgcg gtcgagacgg
    53761 tctggaataa atccggcccg caaggctggg cgcaaacaaa gcggtacggg ctgctattcg
    53821 ggctggcctc agcggccact ctggtcaatc cctacacgta ccacttgcat cgccatatcg
    53881 tggaatttct gcgttccgat ttcatactcc aacatgtagc cgagtttcag tctccgaatt
    53941 ttcgcggcga aggcatgcgc tatttcgaag tgatgttgtt tgccggcctt gccgccgtgc
    54001 cggcgctgct gcggcgcaaa gacgttacgc aagctttttt gctgttgttc tgggcgcacg
    54061 cggctctgct ttccggccgg cacgtgctgc tgtatgtagt cgtcgccgct ccggtggtgg
    54121 cgcgagaagc cacgcagctt tgggaacgcg cggcgaaggc tggaatcggc tggctggcga
    54181 ctctgaagca ggtggcggac gactacgctg gcggctgctt ccatcccgcg ggcggaacgg
    54241 ggcggcgaag agcaattgcc tggatggcgc cggtttgcgt ggtggcgatg ggtgcgacgc
    54301 ttttcgccgg acagcgcagc gagcggctgc ggtcagagtt cccgaagact ctgtttccgg
    54361 tggatgcctg cacggctgcg gagtcccagt tcgccggccg ccgcatcttc acttcggacc
    54421 agtgggctga ttatctgatt taccgctact acccgaagat aaaggtcttc atcgacggcc
    54481 gcagcgattt ttacggcccg caactgggca acgagtacat ccgggccatg aactccgatc
    54541 acgcctgggc gcagatcttc gaccgttacc gctttgacct ggcttt
//
LOCUS       DASREK010000239         9454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_940669, whole genome shotgun sequence.
ACCESSION   DASREK010000239 DASREK010000000
VERSION     DASREK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940669"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1195
                     /locus_tag="VFA54_12690"
     CDS             47..1195
                     /locus_tag="VFA54_12690"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZS51715.1"
                     /translation="MKIAINAISATTGGARTYLLNLARALPSLGRHEYQLYIPGSAAP
                     DLAGLPGNFELLQNARAERSYVQRLVWEQSTLPRRVSRWGANVLICTGNFCPLRSSVP
                     VILLSRNALYFTPLFTSDLLERRHYFWALRHAALSRLGLLSAQAARLTVTPTEAMAKM
                     IRAASGNSPVALRTIPHGFDPWPSGAEAGPPPPAPPFRFLLVSHYNYFRNFETVFRAL
                     ADLRGVYKNGHVKLVLTTDIRPGLRLGGYDTTAAHRLMTDLGLLECVSALSAVPYDGL
                     PRVYGSAHAVICPSYVESFSHTLVEAMGMGIPVIASDIPAHREVAGDAAVFFSPRDPS
                     ELAARCRSVMEDEDLRGRLRAAGRERARHFSWRRHFEELLSVAAEVAR"
     gene            1299..2153
                     /locus_tag="VFA54_12695"
     CDS             1299..2153
                     /locus_tag="VFA54_12695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS51716.1"
                     /translation="MLVPVKNEEANISACLESLRWADEIFVVDSQSSDETARLAEAFG
                     ARVVQFRYQGGPKKKNWSLANLPFRNDWVLIVDADERVTPDLAAEIATCINQPDGPDG
                     YYINRRLIFLNRWIRHAGWYPSWNLRLFRHRLGRYESVAVGSGQVGDNEVHEHVVLQG
                     RAGYLKHDLLHQDYKSLFHYIERHNRYSDWDSAVYLELRRQPLFAQEPFDGAVRRKRM
                     LKRVWVRLPLRPLLRFLYMYVLRAGFLDGAAGLHFCLLASMHEYHISLKMKERLRESG
                     REHCHPHA"
     gene            2122..4008
                     /locus_tag="VFA54_12700"
     CDS             2122..4008
                     /locus_tag="VFA54_12700"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS51717.1"
                     /translation="MDASIVIPTHNKKQELRRCVEAVLAQQFHGEFEVLVCDDGSSDG
                     AAEMIEEWACRDARLRYFRQPKRGPAAARNLGIRHARAPLIAMTDDDAIPQPGWLERL
                     CAALRDPSILGAEGFVASGRPLGPGEAAPSNQTGGVYLTCNAIYRREALALVGGFDER
                     FPFAAFEDCDLAAKIRRHGQIVWVPDAVVLHPPRRATAKNMLQRLRHWPWVMVMGRRY
                     GYLGWPRFPTRHPRARVIWNALVKLPAGRLLAAVRALPSHPAQAFRGILLSLAEPFAA
                     LVIAIPTLLRFDLDSAALHMDYLHLSGQNQKAAGRDAPPAPFPKVGLVIVHYKQPELL
                     RQCIQSFEGAAYPNLHRIVVESAANEADLRSLRRDLPGVEWLAAAENLGYTGGNNLGI
                     AKALEEGCKFILLANADTECIAPDFISRLVRFMDLNPRVALAGPRVYLRRRGEVQNTV
                     LRYPSLLGNIVDWFGFRLFPRRYQRSRDAPLAAKMLNGVCVMLRAEAVRQVGMFDPRF
                     FMYIEDADLGFRLRRAGWQVAYVPVDSLVHHQRRTGYELESEVSLLLRRNSVYFLRKH
                     RRPVQAWGLAAANVVMALVRALTSTSAGAFRRRLAFVRSLCRELRFVLAIPARELQRR
                     LPAP"
     gene            4056..5387
                     /locus_tag="VFA54_12705"
     CDS             4056..5387
                     /locus_tag="VFA54_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HZS51718.1"
                     /translation="MMPQSRQTFLSAVFWNAVLMAVALPAAIAASIVVGRVLGPAGKG
                     EFTLATLIGALLLTVLNLGIPAAISYFISGGHVPEASFVKTVVVLGAALAAIGFVLTL
                     SLDRAGCASRLLGTPHLTAAMWLVFIGLPFQFWGTFLQFVILAQGRRVLFAALPALGQ
                     LFVTVLIGILALLGRLTPVSAVAVTVASQILTGSTLLVFSQLHLDWLRAPLLSPRFWS
                     SAAKYSLTSYGANTLQFLIQRADVFLVSIFLGVRAVGLYSVAYGVAELLTLLPQRLNN
                     LYVPRIAGERTPDAKRQEVPLSSSLVFVSTAAAAVVLALTAPFCIRFFYGEQFAPSVT
                     PLLLLLPGICALAASSIQTAYLSGVGRVGTNAAAAGAGLALNLLLNLVSIPRYGISGA
                     AAASSAAYCAQALLLVQAVARLTNQRPLAMLTSAPPAVFAGYVKRAFRGAP"
     gene            complement(5494..7383)
                     /locus_tag="VFA54_12710"
     CDS             complement(5494..7383)
                     /locus_tag="VFA54_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51719.1"
                     /translation="MISLAGYMAVPWPAAKLVLASAAAAAAVTSPGPAFPPAVRRAAW
                     GACFALAFLILPYWLYFGGKQLAPPLQILWFAAMAAAGRLWAKALEAVTGDSRPLPPL
                     TWWKAAAAAAALLCISWPAFFQVVPSRGDESQHILRLWEWLLFVRSLLQSWTGRLALG
                     CFAATLVLVRKKWRIAAAAALGCAMGLWICAILLTASSVDMNWLPRYPGLQIWWQATA
                     LAYAPIKSWIAQEALYRVTPFLAVLGLALYAMRGPLAPCSAPIQLGAAFLIATAPAVR
                     YYSTLLYLDLPLAAAMTIVCFEIEELAAFSRTGKIPGSAGWYALLLAGFLKETALIFI
                     ATSLLFCAWGRRRLTIAWAVGAPVALFLVSAGVQRGYAFRPANWATFSNYKILAGALW
                     EQYGWLLLLAAIGVVLLAKERRVLLIAFGLAVGVAYTLFYLGDSAIVLQQGVWRPKYL
                     GHARFMLAWLPALAAFSAQALRLLPPKIAWACAGIVLAGNLWVYPVSTMAAPNSHWGD
                     YVYDTGDHFYPYNIAFERGRSEFPQARRVIVIGADFYYPYVFYQRKWSWPAQIDSIVL
                     PTGYSEDAYRRSAAEAAARKPDLIWVHVMDHLPGAVYESAPSGYAPAGDYRSRFHRIL
                     AFRRP"
     gene            complement(7425..8573)
                     /locus_tag="VFA54_12715"
     CDS             complement(7425..8573)
                     /locus_tag="VFA54_12715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS51720.1"
                     /translation="MKIAVLASWFRRGGGGAFVHLSEIYRRLPHEFVIIGDQQPEDEQ
                     GDRDLPWRVIRVPVGVRRSIWKLNTALAFARGSSAVENLVAAEKPDLIHAGYYLPWGL
                     YAARLRQSRQIPYALFTWGEDLLAAEHDWLRRRAMQRAAREAKWLFTNSEFSTERLRN
                     FAPRETPVHTVFGGADLNRFRPDLPRDVIRRRLGLGHESLILLSVGALRPQQGVDRLL
                     DAVARLANRFPTLRYVVAGKGYYEEALRRRAADLGLAKRVAFAGYVPDEELPLYYAAC
                     DVFAMLHRRVEETGEEMCFGLVFLEAGACGKPVIGSRLGGTHFAIRDGATGYRVDPES
                     PSEIDERLSALLSDAALRARLGAEARSWIESTFSWDRAAQLVREAHGW"
     gene            8681..>9454
                     /locus_tag="VFA54_12720"
     CDS             8681..>9454
                     /locus_tag="VFA54_12720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS51721.1"
                     /translation="MPTQRVQTDTAQSAPSRRLELPSSQAGNRAAPVRYLSAAAGAAR
                     LHPDAAALLAVTGAYLCYASLFFGKMHNFEVDVGTDFALADLLSQGKILYRDIAYRYF
                     PLAPWVNAALFRLLGRSTDVLLGAATAASFGIVILVFLLGREILPPAPAMVASLIVLN
                     SAVYGPHLMSYATPYSFACTYGLLFSLIALYGACRTLDTAYSRWDFITGCAAGLGMAS
                     KHEFAIINALIVGVNLAFRFSAHGRRILRGVFASAGVCAA"
BASE COUNT         1666 a         2985 c         2910 g         1893 t
ORIGIN      
        1 tgccgaccgg gggagcgcca gactgcccgc cgcatccgcc gtttagatga aaatcgccat
       61 caacgccatc tcggcgacta cgggcggcgc gcgcacgtat ttactgaacc tggcgcgggc
      121 cctgccctcg cttggccgcc acgaatatca gttatatatt ccgggcagcg cggcgcccga
      181 tctggccgga ttgcctggaa atttcgagct tttacagaac gcgcgggccg aacggagcta
      241 tgtccagcgc cttgtctggg agcagagcac gctgcctcgc cgcgtctccc ggtggggagc
      301 caatgtgctg atctgcacgg gcaacttttg ccctttgcgt tcttccgtgc cggtcattct
      361 tctgagccgc aacgccctgt actttacgcc gctgtttaca agcgatttgc tcgagcggcg
      421 gcattatttc tgggccttgc ggcatgcggc tctctcccgc ctgggcctgc tctcggcgca
      481 ggccgcgcgg ctgaccgtta cgcccaccga ggccatggcg aagatgatcc gcgcggcatc
      541 cggaaacagc ccggtcgctc tgcgcaccat tcctcacggc tttgatccct ggccttccgg
      601 ggccgaggcc ggccccccgc ctcccgcccc gcctttccgc tttctcctgg tgagccacta
      661 caactacttt cgaaacttcg aaaccgtatt ccgcgcgctc gccgatctgc gcggcgttta
      721 caaaaacggc cacgtgaagc ttgttcttac aacggacatc cggccggggc ttcgcttggg
      781 aggctacgat acgaccgcgg ctcaccgcct tatgacagat ctcgggcttc tggagtgcgt
      841 ctcggctttg agcgccgttc cttatgacgg tctgccccgc gtgtacggct cggcgcacgc
      901 cgtgatctgc ccctcctacg tggaatcgtt cagccacacc ctggtcgagg ccatgggcat
      961 gggaataccg gtgatcgcgt cggatattcc cgcgcatcgg gaggtggctg gcgatgcggc
     1021 cgtctttttt tcgccccgag atccatcgga actggcggca cgctgccgct ccgtaatgga
     1081 agatgaggac ctgcgcgggc gcctgcgggc tgcggggcgc gagcgagcca ggcatttcag
     1141 ttggcggcgg cattttgaag agctgttgtc ggttgccgcc gaagtagccc gttagcatgg
     1201 caaaccatcc gccagagaca gcagcggacg acgccgggct tgcgccggcg gctcctggag
     1261 cgaagccgcg gccgcaagcc gcgcggcaaa aagtttccgt gctcgtgccc gttaaaaacg
     1321 aagaggccaa tatctccgcc tgcctggaat cgctgcgctg ggccgacgaa atatttgtcg
     1381 ttgactccca atccagcgac gagaccgccc gcctggcgga agcgtttggt gcgcgcgtgg
     1441 tgcaattccg ttaccagggg gggccgaaaa agaaaaactg gtcgcttgcc aacctgccgt
     1501 tccgcaacga ctgggtgctg attgtggatg ccgatgagcg cgtcacgccc gatctcgccg
     1561 cagaaattgc aacctgtatc aatcagccgg atgggccgga tggctactac atcaaccgcc
     1621 ggcttatttt cctgaaccga tggattcgcc acgccggctg gtacccgagt tggaatctgc
     1681 ggctgttccg tcatcggctg ggccgctacg aatcggtcgc cgtcggctcc ggccaggtcg
     1741 gcgacaacga agtgcacgag cacgtggtct tgcagggacg ggctggctac ctgaagcatg
     1801 atctcttgca tcaggactac aaaagccttt tccactacat cgagcgccac aaccggtatt
     1861 ccgactggga ctcggcggtc tatctcgagc tgcgccgcca gcctcttttc gcgcaggagc
     1921 ccttcgacgg cgccgtgcgg cgcaagcgca tgttgaagcg ggtctgggtg cgcctcccgc
     1981 tccggccgct gctgcgcttt ctgtatatgt acgtgctgcg cgcgggattt ctggacggcg
     2041 ccgccggcct gcatttctgc ctgctcgcca gcatgcatga gtatcacatc agcctcaaga
     2101 tgaaagagag attgcgggaa agtggacgcg agcattgtca tccccacgca taacaagaag
     2161 caggaactcc ggcgctgcgt ggaggccgtg ctggcgcagc agttccatgg agagttcgag
     2221 gtcctggtgt gtgatgacgg ctcaagcgat ggcgccgcag aaatgatcga ggagtgggcc
     2281 tgccgcgacg cgcgcctccg gtatttccgg cagccgaagc gcgggccggc cgcggctcgc
     2341 aatctgggaa ttcgccacgc gcgcgcaccg ctcatagcga tgaccgacga cgatgccata
     2401 ccgcagcccg gctggctcga gcggctgtgc gcggcgctca gggatccatc cattctgggc
     2461 gccgaaggat tcgttgcttc cggccgcccc ctgggccccg gtgaagccgc tccgtcgaat
     2521 caaaccggtg gcgtgtatct gacctgcaat gcgatctacc gccgggaggc cctggcgctg
     2581 gttggcggct tcgatgaacg gttcccgttt gccgcctttg aggattgcga cttggccgcc
     2641 aaaatccgcc ggcatgggca gatcgtgtgg gtcccggacg ccgttgtgct gcacccgccc
     2701 cggcgggcga cagcgaagaa catgctccag cgcctcagac attggccctg ggtcatggtg
     2761 atgggcagac gttacggtta tcttgggtgg ccgcgctttc ctacgcgcca tccgagggcg
     2821 cgggtgattt ggaacgctct ggtgaagctg ccggcgggcc gtctgctggc ggcggttcgc
     2881 gcgctcccca gccatccggc gcaggccttc cgcggaattc tcctctcgct ggcggagccg
     2941 ttcgccgccc tcgtgattgc cattccgacc ttactgcgct tcgatctcga ttctgccgcc
     3001 ttgcacatgg actatcttca tttgtccggg cagaatcaga aagccgccgg acgggacgcg
     3061 ccgcctgcgc cattccctaa agtaggtctg gtgatcgtcc attataagca gccagagctg
     3121 ctgcgccagt gcatccagtc cttcgaaggc gctgcctacc caaacctgca ccggattgtt
     3181 gtggaaagcg ctgcgaatga agccgatctg cgcagcctgc gccgtgacct gccgggcgtg
     3241 gaatggctgg ctgccgctga gaacctgggc tatacgggcg gaaacaatct cggcatcgcc
     3301 aaggcgctcg aagaaggctg caaattcatt ctgttggcca atgccgacac cgagtgcatt
     3361 gcgccggatt tcatctcccg gctggtgcgt tttatggatc tcaacccgcg agtcgcgctc
     3421 gccggccctc gcgtctatct gcggcggcgg ggagaggtcc agaatacggt tctgcgctat
     3481 ccctcgcttc tggggaatat tgtggattgg ttcggcttcc ggctgtttcc ccggcgttat
     3541 cagcgctcgc gtgacgcgcc tctggcggcc aagatgctca acggcgtttg cgtaatgctc
     3601 cgcgccgagg ccgtccggca agtgggcatg ttcgatccgc gcttcttcat gtatatcgag
     3661 gacgccgatc tcggctttcg tcttcgacgc gccggatggc aagtggcgta cgtgccggtt
     3721 gacagcctcg tgcatcacca aagacgcact ggatacgaat tggaaagcga ggtcagcctt
     3781 ctgttgcgcc ggaactcggt ttactttctc cggaagcatc ggcgcccggt tcaggcatgg
     3841 ggcttggcgg cagccaatgt cgtcatggcg ctggtgcgcg ctctgaccag cacttcggcc
     3901 ggcgctttcc ggcggcgtct ggccttcgtt cgctccctct gccgggagct gcggttcgtt
     3961 ctggcgatcc ctgctcgcga attgcagcgg cgcctgccgg ctccctgaat gcttgtcgtt
     4021 tcactctggt tcgaaagagg cagaatggca agccaatgat gccacagtct cggcaaacat
     4081 ttctttcggc tgtcttctgg aatgccgttc taatggcggt tgctcttccc gccgccatcg
     4141 ccgccagcat tgtcgtgggc cgggtcctgg gtcctgccgg caagggtgag ttcacactgg
     4201 cgacgcttat cggcgcgctg ctgctgaccg ttctcaatct ggggattccc gcggcgattt
     4261 cctacttcat aagcggcggc catgtccccg aggcctcgtt cgtgaaaaca gttgtggttc
     4321 tgggggccgc gctggccgcg atcggattcg tgctgactct gtcgctggat agagccggat
     4381 gcgcgagccg actgttgggc acgccgcatc tcaccgctgc tatgtggctc gtctttatcg
     4441 ggttgccgtt tcagttctgg gggacttttc tgcaattcgt cattctggcg caagggcgcc
     4501 gcgttctgtt cgccgctctg ccggctttgg gccagctatt tgtaactgtt ctcatcggaa
     4561 tcctcgctct cctgggcagg ctgacgcctg tttcggccgt ggctgtaacc gtcgcctcac
     4621 agatccttac tggaagcacg ctgctcgtct tttcccagct tcatctggac tggctgcgtg
     4681 cgccgctgct ttccccccgc ttctggagca gcgcggcgaa gtactcgctc accagttatg
     4741 gcgcgaatac gttgcaattt ctcatccagc gagccgatgt ttttctggtg agcatcttcc
     4801 tgggagtgcg tgcggtgggt ctctactcag tggcgtacgg cgttgcggag ctgctcacgc
     4861 ttttgccgca gcgtctgaac aatttgtacg tcccgcggat cgccggcgag cggactccgg
     4921 atgcaaaaag gcaagaggtg ccgctctcgt cgtctctggt gtttgtttcc acagcggcag
     4981 cggcggtcgt gctcgcgctc acagccccct tctgcatacg gttcttttac ggcgagcagt
     5041 tcgctccgtc tgtgacgccg cttcttcttt tgctgccggg catctgtgcg ttggccgctt
     5101 cttcgatcca gaccgcatat ctatcgggcg ttggccgcgt gggcaccaat gccgcggcgg
     5161 caggcgcggg cctggcgctg aatcttcttc tcaatctcgt ttcaattcct cgctatggaa
     5221 tttccggggc ggcggcagct tcttcggcgg cctattgcgc gcaagcgctc ctgctcgtac
     5281 aggccgtcgc gcgcctgacg aatcaaaggc cgctggcgat gctaacctcg gcgcccccag
     5341 ctgttttcgc cggctatgta aaacgagcct tccgtggcgc gccctgatgg tttccgcgtt
     5401 ctggacggcc acggccattt tgcccctttg gtcctgggcc ggccggcgaa cttttgttag
     5461 gtggctcaaa ctggtgtgtg cggctcaagg tggctacggc cttcgaaaag ccagaatccg
     5521 gtgaaatcgc gagcgataat cgccggccgg agcgtagccg ctcggcgctg attcatatac
     5581 ggcgccagga agatgatcca tcacgtggac ccagatcagg tccggcttgc gggccgccgc
     5641 ttcggccgca cttcgccggt acgcatcctc cgagtagccg gttgggagca caatcgagtc
     5701 gatctgtgca ggccagctcc acttcctttg atagaacacg taaggatagt agaaatcggc
     5761 gccgatgacg atcacgcggc gagcttgggg aaactcggaa cggccgcgtt cgaaagcaat
     5821 gttataggga tagaagtgat cgccggtgtc ataaacataa tcgccccaat gcgagttggg
     5881 ggcggccatc gtgcttaccg ggtagaccca cagattcccg gccagaacga ttcccgcaca
     5941 ggcccacgca atttttgggg gcagcagccg gagcgcctgg gcgctgaatg cagccagcgc
     6001 cggcagccac gcgagcatga agcgcgcgtg tcccaggtat ttcggccgcc aaacgccttg
     6061 ctgaaggact attgcgctat cgcccaaata gaacagcgtg taagcaacgc ccacagccaa
     6121 gccgaatgcg atcagcagca cgcgcctttc cttggccagc agaacaacgc cgatcgccgc
     6181 gagcaacagc agccacccgt attgttccca aagagcgccg gcgaggattt tgtaattcga
     6241 gaaggtcgcc cagtttgccg ggcgaaatgc atatccgcgc tgcaccccgg cgctcaccaa
     6301 aaacagcgcg accggcgccc cgacggccca tgcaatcgtt aaccggcggc ggccccaggc
     6361 gcaaaataga agagaggtcg cgatgaaaat cagcgctgtt tctttcaaga agccggccag
     6421 cagcagcgca taccagccgg cggaacctgg aatttttccc gttcgcgaga atgcggccaa
     6481 ctcttctatt tcgaagcaga caatggtcat cgccgccgcc agcggaagat ccagatacag
     6541 gagcgtcgag tagtaccgca cggccggggc cgtggcgatc aaaaatgccg ctcccaactg
     6601 gatcggagcg gaacatgggg ccagcggccc tcgcatggca tagagcgcca ggcccagcac
     6661 tgcgaggaaa ggcgtcactc tgtagagcgc ttcctgggca atccagcttt tgatcggcgc
     6721 atacgcgagc gctgtggctt gccaccagat ttgcagtccg ggatagcggg gcagccagtt
     6781 catgtcgacg cttgaggcgg tcaatagaat ggcgcatatc cacaagccca tcgcgcagcc
     6841 gagggctgcg gcagcggcaa tacgccattt tttgcgcacg agaacgagag tggcggcgaa
     6901 acatcccagg gccaggcgcc ccgtccagct ttgcaacagc gatcggacaa aaagaagcca
     6961 ttcccagagc cggagaatgt gctgcgactc gtcgccccgc gaaggcacaa cctgaaaaaa
     7021 agccggccag cttatacaga gcagagcggc tgccgccgcg gccgctttcc accacgtcag
     7081 gggtggcagg gggcgggaat ctccggtgac cgcttccagc gctttggccc aaagccgccc
     7141 tgcggctgcc attgccgcga accaaagaat ctgcaagggc ggggccagtt gctttccgcc
     7201 gaaataaagc caatagggaa gaataagaaa agcgagtgcg aagcaggccc cccaggcggc
     7261 gcgccgcaca gccggaggaa atgccgggcc cggcgacgtc acggcggcgg cggctgcggc
     7321 ggaagccaga accagcttcg cggcgggcca tgggacggcc atgtagccgg ccaggctgat
     7381 caatgagatt ctccaagcga gaggcaggcg ggcggaccac tctgttacca accgtgggcc
     7441 tccctcacca gttgagccgc gcggtcccaa ctgaacgtag attcgatcca ggatcgcgct
     7501 tctgccccca accgggcgcg aagcgccgca tcggagagca gggcggacaa tcgctcgtcg
     7561 atctcggatg gagactcggg gtccacacgg tatccggtcg cgccgtcgcg aatagcaaaa
     7621 tgtgtgcctc cgagacggct tccgatcacc ggtttgccgc aagcgccggc ctccagaaaa
     7681 accaggccga agcacatttc ctctccggtt tcttccaccc ggcggtgcag catggcgaac
     7741 acatcgcacg ccgcgtaata gagaggaagc tcctcgtccg gaacgtagcc ggcaaaggcg
     7801 acgcgctttg ccaggcccaa atcggcagcc cggcggcgca atgcctcttc gtaataccct
     7861 ttgccggcga ccacatatcg gagagtggga aagcggtttg ccagccgtgc cacagcatcc
     7921 agaaggcggt ctacgccctg ttgcggtcga agcgccccca ccgagagcag tatcaggctt
     7981 tcgtggccga ggccaagccg tctgcgaatt acatcgcgcg gcaaatcggg ccggaaccgg
     8041 ttcagatccg cgcccccgaa aacggtgtga accggcgttt cccgaggcgc gaagttcctc
     8101 aggcgctcgg tgctgaattc gctgtttgtg aatagccact tggcctcccg cgcggctcgc
     8161 tgcatggccc ggcggcggag ccaatcatgt tcagcggcca gcaggtcttc gccccaggtg
     8221 aacagagcat aaggaatttg cctcgattgc cgcagccggg ctgcatacag gccccacggc
     8281 aggtaatatc cggcgtgaat cagatccggc ttttccgccg ccaccagatt ttccaccgcc
     8341 gagctgcccc gcgcgaacgc gagagccgta ttcagcttcc atatcgagcg gcgcacgccg
     8401 accggaacgc gaatcaccct ccagggcaag tctctgtcgc cttgctcatc ttccggttgt
     8461 tggtccccga taatcacaaa ctcgtgcggc agccgccggt aaatttccga aagatgtacg
     8521 aacgcgccgc ctccgccgcg ccggaaccaa ctggcgagaa ccgcaatctt catgagacct
     8581 tcgcgcagaa cgtgacagcc attatagccg agccggacga agcggcattt tcggcggatc
     8641 gcgtgtcgcg cgtgagaatg cgggaggcgg cgcaaaaata gtgccgacac aacgagtgca
     8701 aaccgacacc gcgcaaagcg ctccttcccg ccgcctggag cttccatcca gtcaggcggg
     8761 aaatcgagcg gctccggtac gatacctcag cgcagcggcg ggcgccgcca ggttacaccc
     8821 ggatgcggca gcgctgttgg ctgtaacggg tgcgtatctc tgctatgcgt ctcttttttt
     8881 cgggaaaatg cacaacttcg aagtggatgt cgggaccgat tttgcgctgg ccgatttgct
     8941 ctcgcagggg aaaatcctgt accgtgatat cgcctatcgc tatttcccgc tcgcgccctg
     9001 ggtgaatgct gcgctgtttc gcctgctggg ccgctctact gacgttttgc tgggggccgc
     9061 cacggccgcc agtttcggca tcgtcatcct ggtcttcctg cttggcagag aaatcctgcc
     9121 tccggcgccg gcgatggtgg cgtcgctcat cgtgctgaac tccgccgtct acgggccgca
     9181 cctgatgagc tatgccactc cttacagctt cgcctgcacg tatggattgc tgttttcctt
     9241 gattgcactc tacggcgctt gccggaccct ggatacagca tattcccgct gggacttcat
     9301 aacaggctgc gccgcagggc tcggcatggc atcgaagcac gaatttgcga tcatcaatgc
     9361 tctgatcgtg ggcgtgaatc tggcttttcg gttctctgcg cacgggcggc ggatcttgcg
     9421 aggcgttttc gccagcgcgg gagtctgcgc cgct
//
LOCUS       DASREK010000240        19223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1440289, whole genome shotgun sequence.
ACCESSION   DASREK010000240 DASREK010000000
VERSION     DASREK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 19223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19223
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440289"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFA54_12725"
     CDS             complement(<1..628)
                     /locus_tag="VFA54_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZS51722.1"
                     /translation="MPVKIYDAIVVGSGATGGWAAKQLTEGGMEVLLLEAGRKLDPAK
                     DFLEHAWPYDMPFRGFGRPMDLEVRQGSAAGFSSEYTTHLFVNDADVPYTTPPDKPFN
                     WVRSRQVGGRSITWGRQSYRYSNYDFQAASHDGYGVNWPFRYEDLAPYYDLVEDFIGV
                     SGRAEGWEALPDGRFLPPMKYTCGELELKKAVDRHGDRILTIGRTAILT"
     gene            complement(721..1278)
                     /locus_tag="VFA54_12730"
     CDS             complement(721..1278)
                     /locus_tag="VFA54_12730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZS51723.1"
                     /translation="MSNIDRRAALKLIAAGVLAERLAEAQHHLETIARAPGAYKLQFF
                     TPAQNALLDQLSELIIPADGRSPGAREAKVSLFIDLMVANSRKDVQQQWISGLQAVES
                     EAKNRFRNRFLDCNAGQQDQILRAMAANENKPANDLERFFAALKLMTINGYYTSPVGI
                     HKDLQYIGNTAQVDFPGCAAPEQRG"
     gene            complement(1312..2532)
                     /locus_tag="VFA54_12735"
     CDS             complement(1312..2532)
                     /locus_tag="VFA54_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZS51724.1"
                     /translation="MSETQRALVERLMAACGGSYSPEVRRRHFISGPQWAAAYQGQAL
                     FHAPTRDPITYRDVIREYSRIGIGYWSTHDTDVIPAGTLLTERQWEIVDDIKRALDEH
                     GVRCSMVTTETFHHPVWAASSAAEAPEVRQYAAERLANTVEIGRRLGAQFVVYWPGTL
                     GYLVQGAIEETRTLRWFADGINAACARDREVARRDGRPTLRHCLEAKPFEPQAEILLP
                     TTDAMLAFIASGLLTHPEMVGVNPEYLHELMWGGAPRAALARALLAGKLWHFDINDGY
                     RLKHDVDIAVGLVNPLDWLNVLVLLRLHNYNGPFNLDYKPPRTTSNWGVFHVSFPAAV
                     DRFITLWEMAGEVISDPILGEAAAALRAGGNGSGPVGNIVEANRELLTLHELIGHRIF
                     QILIGQHGGRLYSL"
     gene            2589..3941
                     /locus_tag="VFA54_12740"
     CDS             2589..3941
                     /locus_tag="VFA54_12740"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZS51725.1"
                     /translation="MREVLERRFRLREHETTISREIVGGVTTFLTMSYILFVQPSLLS
                     QAGMDFQAVLVATCAASAAATLLMALAANYPVALAPGMGENFFFVYTLCGSYRLTWQQ
                     ALAAIFTAGAAFMALAAFGLRSRIVNAIPNALKTGIAAGIGLFISLIGMQYGRLVVAN
                     PATLLQLGDLRSPIALITMAGLLLTLGLLAFGVRGAILLGILGSTAVAAGFGRVHYSG
                     IVAAPAGLSATLWKMDFAGLFHRDPATVAVVVFVLFFLALFDTVGTLVGVGRQAGLMR
                     NGALPRAERALFSDATGTVVGAALGTSTVTCYIESAAGVSDGARTGLASMTTGTLLLI
                     SMLFAPLARMIGGGVPVPSAQGGGVAYPTLAPALILVGAMMMRCARDMDWEDPTESVP
                     GFLTMILIPFSFSIAAGVGAGFVLYAAAKLLAGRPKECPAVVYVFAALYVIQYAVARQ
                     "
     gene            complement(3990..6932)
                     /locus_tag="VFA54_12745"
     CDS             complement(3990..6932)
                     /locus_tag="VFA54_12745"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZS51726.1"
                     /translation="MKHPSPDLEAELRKEIEGEVRFDAYSKVLYSTDASIYQIEPIGV
                     VVPRHRDDVAAAVRVAARYGIPILPRGGGTSLSGQTVGHAIQIDFAKYMRNVLEVNTE
                     ERWVRVQPGVVQDQLNAYLRPYGFGFGPDTSSSNRATIGGMIGNNSAGSHSILYGKTI
                     DHTLELDVVLSDGSEARFREVTPERLEQLSRGDALENRLYREVRALGAAYCSEIFARY
                     PKIIRRVSGYNLDEFVPAPGAERPGIFGAGFATRPGLFNLCKAIVGAEGSLAVASEAK
                     VRIVPLPQKKAIEIVLFDDLVDAVEATDEMTVTSPVATELLDRLIIERARATPQFAKQ
                     MDFVQGDPGALLIVEFYGDTEAEVRSKVEELDARLKRKRIGFGHLPVVDPARQASIWK
                     VRKDSLGLLMSVPGDRKPIAFVEDPAVPVERLPEFVRRFRQILADHDTTGGYYGHASV
                     GCLHIRPLIDLKQESEMYKMRDIAEEVFQLVVECGGSMSGEHGDGLARGHFNARLFGP
                     KIYEAFQKLKAIFDPQNIMNPGKVVNAPEMTQDLRYGKTYQTIQIQTHLDFSREGGFA
                     GAVEMCNGMGICRKTLEGTMCPSFHATREEEHSTRGRANALRAALSGRLPAEELTSRR
                     MYETLDLCLECKGCKRECPSNVDLAKIKYEFLAQYYAKHGVPERALLFANIGKLNRWG
                     SRMAGVANYMMSRRWAKNLAEMAFGIDARREFPAFASETFQSWFAKRAGGNGRANGRA
                     ARGAVVLFDDCFLSYNYPQVGRAATELLERAGFEVILAKHDCCGRPMISKGLLEDARR
                     AARRNVEALVPLVEKGAVVVGCEPSCLLTLRDEYLDLLQGHAAERVARQSYLFEEYLC
                     KLEDEGKLGLQWRQERKKILLHGHCHQKAHIGTASTLQALRFPAGYEVSEINSGCCGM
                     AGSFGFEKEHYDISRIIAETRLIPAVNAASPDTEIVITGVSCRQQISHFTRRTPRHAA
                     EVLRDALA"
     gene            7109..7633
                     /locus_tag="VFA54_12750"
     CDS             7109..7633
                     /locus_tag="VFA54_12750"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS51727.1"
                     /translation="MILRRLCALLASFCSLLCAAGELSNRPAPDFHLSDSQGRMVSLS
                     SFKGKVLLVEFLSTTCPHCQKFAPVLDSVYTRLKGRVAVVGISTFPDTAETVAEFVKT
                     YKISYPVLVDPTNKAAIDYLKPDPNHGFSIPHVFVVDQSGYIRDDFVQNPSNAELFTP
                     EGLTRLLRAYLTSK"
     gene            7805..8125
                     /locus_tag="VFA54_12755"
     CDS             7805..8125
                     /locus_tag="VFA54_12755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZS51728.1"
                     /translation="MKEQRRSQRFQLTLPLTLIRGSRGEINRIGETRNLSSTGVYFVV
                     GEAVELGSLLEFVVTLPDEISIAGPVRLLCKGKVTRVEQQEASLVGVAATIERYEFVR
                     DNVN"
     gene            8306..8962
                     /locus_tag="VFA54_12760"
     CDS             8306..8962
                     /locus_tag="VFA54_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZS51729.1"
                     /translation="MSKIDSDDHGKTKQPGKKGFSRRSFLQGVGIGGGAVASLPTGLL
                     VEPEVASAAPVMGPGPVRVSLRINGKVHNLNIEPRVTLLDALRNHLDMTGAKKVCDRG
                     TCGACTVILNGRAVYSCSVLAIEAQGKEIQTIEGIGSLSKLHPVSAAFVENDAQQCGF
                     CTPGFVMAVKAFLDRHPNPTMDQVKRGLGGNLCRCGTYVGVRQAALDAAKEMKGGRRA
                     "
     gene            8955..11111
                     /locus_tag="VFA54_12765"
     CDS             8955..11111
                     /locus_tag="VFA54_12765"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZS51730.1"
                     /translation="MPSKYDWPPAEKRSLIGKRVNRLDGPHKSSGVARYAYDTNPPGL
                     LTAKMLTSPYAHAKVTRIDTSEAERLPGVRAVEVIAPAGTELQWEGQEIAAVAADTEE
                     LARDGVRKIKVEYEVLPHLVHEYDLTKAGNRAKPSAEETKGNPDQAFSSADVVHEGEY
                     GINTECHCCLEAHGQVVEWNGDNVTVTASTQAVGRLGGDLAKVLASSPGTENIKPSNI
                     RVITQYMGGGFGSKFSIDTWGIACTKLARRTGKPVKLMLERDQELQMAGMRPSDFAKI
                     KVGAKKDGTVVAWESESWSTGGMAGGGAPPLPYIFADIPNQKKRHIAVSTNTGPARAW
                     RAPNHPQAAVLTMNALDDLAAKLNMDPVEVFMKNLHYTPRPDVYEAELKKAMELMDWK
                     AKWHPRGQGGNGHIKSGVGVSIHTWGGRPHDSDCRATINPDGSCNVALGSQDLGVGCR
                     TVIGIVAAETFGLPLSAVEVLIGDSNYPPSGASGGSTTTGGVSASTRRACIKALDKLF
                     EAVAPSLGTTPDKLVARDGRILVEGDPSKSLTWKAACAKLGSKPISEIGRQPARGEGN
                     LNDSNVGGVGMAEVEVDTETGIITMKKYVAVQDCGLVISLKTAESQVFGAAIMGICSA
                     LYEERIYDQQVGKMLNANMEFYKLSGIGDIGDIVVHMVQGPYDSRGVIGLGEPPAVSP
                     PAAISNAVANAIGVRVQPLPMTPDKVLAALARGGRA"
     gene            11111..12097
                     /locus_tag="VFA54_12770"
     CDS             11111..12097
                     /locus_tag="VFA54_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HZS51731.1"
                     /translation="MQAFEYASPKTTKEALGLLGSQWGETDILAGGTDLLALMKDYIH
                     TPRRVVSLGGIKELKTIKSSGDGLHIGAMVTFAELIENSTISKEYPTLNQAAHGVSSQ
                     QIRAMGTVGGDLCQRPRCWYFRNGFGLLGQDGKGNPLVPNGENRYHAILGNSGPAKFV
                     SASSLGPPLISLGAKVVIASSSGNRTVDVAKFFVTPENANMREVDLKPNEILTEIIIP
                     PHGGAKTATYEVRQKMALDWPLTTASVALKMSGSTVQSAVVVLGHVAPTPWNSPEAAQ
                     AITGKSVTDATAQAAADAALARATPLSKNAYKVQLAKVAVKRALLLAATGRA"
     gene            12101..12313
                     /locus_tag="VFA54_12775"
     CDS             12101..12313
                     /locus_tag="VFA54_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51732.1"
                     /translation="MENAGLKVLAERCGNLRWKGLFIDAEWDPTVQQSNDRIYWCVQT
                     QNPLGPDGQVVDEDVCNPNRSCYEAL"
     gene            12367..13041
                     /locus_tag="VFA54_12780"
     CDS             12367..13041
                     /locus_tag="VFA54_12780"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HZS51733.1"
                     /translation="MRKVLLTATLLLASAAMLSAAEIRGSYIEARNADIWVAQCFANS
                     EVGIVGDLALMGWKIDKGSWNNVSLDGMGVVAVVRAKSTLGDTFHSAYPTKAILIVDE
                     RANMEQRQALQSFVQKMAGDLVQTVVRVESAPIEFNIGGSIHSGSAQLVAGNLARIQT
                     RALREGDAVCHNAITYYPPLSANLEHSMPAHTVANTFSGQGLNVVWSNPDKNSAFLGS
                     FATESD"
     gene            13121..13768
                     /locus_tag="VFA54_12785"
     CDS             13121..13768
                     /locus_tag="VFA54_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51734.1"
                     /translation="MPALKSFAPLFLFLILLAPARAQYTVAPIGACDASGVSDAVKNV
                     LEPNGYQVKDASGAPYAEVWLRKGIPSDKNAPSAGGADFTSIPLSTLIGVIRFDKDTN
                     DFRGNKVRAGVYTMRYQLQPEDGNHQGASPRRDHLVLSLAADDTDPNANPNFDALIKQ
                     SEKASGINHPLVLFLALPDPNAKFPSIEHVSSRDMLDVQNGSLQLGITVAGKAEE"
     gene            13994..15931
                     /locus_tag="VFA54_12790"
     CDS             13994..15931
                     /locus_tag="VFA54_12790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS51735.1"
                     /translation="MRALSRLDIALLSLCLALFLFFGAAWISLPGPQQDELLHISVLI
                     PRLRGLETFSLKTKHHQYPLMMMPYVGALKGWLLWLWFRVVPMGVVGYRGFGIAAGAV
                     TVWLIFWFVRRHYGDTVSLLTTGLIATDPVFVQGIRLDYGPVALMHLLKVGGLCLLSI
                     WLASRSRWAAAAGMFLFGLALWDKANFAWFLAGLSVTMAILFSREAIARLRAQPQLLL
                     IACAAFLIGAAPLLVYNWKRHGETWRERGRFEVRWFKLIEASKTFNGRFMLDMTAGDQ
                     YDNSPPAADVFLPGLSQWMYRLGRLRQTITLPLLAAALALLPLNLRLTRSRRSLLFPL
                     LLSALTYACMFFSRDGGASAHHVIMIQPFPMLFLAVSLWTPAERRPSLAARAAATVVV
                     LAAIAVNISLNARHLAVYTRTGGTRAFSDAIYRLVPYLARNSGWRLYAMDWGFSNPVA
                     FLGRRWNLRVDDFFFAVNGDSESSRINEEQLARLMRDPGNVFLLHSPPRVFFPNPGEK
                     FFALASSGIPMREIAEFRERSGEVVYQIYRCEEPDSPGKALSEVEVRFTPQQVVPRQQ
                     YTIEVREIHDSWIDLVYDVNQTRGAANRFCYLDAQGRAKMTVPAGYPAATVRILRIRP
                     SGGEWRPARGSITVVGGTQNP"
     gene            15928..17460
                     /locus_tag="VFA54_12795"
     CDS             15928..17460
                     /locus_tag="VFA54_12795"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZS51736.1"
                     /translation="MTGAELFVERLKDHGVRWVSTLCGHGLDPLFDACRRAGLPLIDT
                     RNEQAASYMADAWGKLTRAPGVVAASSGVAHANALAGVVNAYFDGSPMLLVTGAHAVA
                     TMGHGHFQDLDQVALAAPVCKSARLIDRPETIAPLLEEALLAAVAPRPGPVHLTFPMD
                     VQRAEAPSGERIARRLREPQSRSPAPLYTLPASYSRPLLIAGSGVFYAQKEQALARFC
                     EKSAVPVVTPIWDRGCVNQAIPQFMGVIGAASGGPRLLPDADLILLCGAAADYRVGYL
                     QAPAIHPAAHVVKIEPQHLETLELAPQPAWLAEAQRRRDEFRRAFASVPRQRAGIHAL
                     DIIEALRGVLTEETVLLIDGGSIGQWCHQLLCDRYPGHWLTCGASGTVGWGLGGAMAA
                     RLRFPDRPVILVSGDGAATFTIGELECAVRQRLPFVLVVADDQAWGITRTGHEREFGQ
                     PIASELGPIDFATLAESLGARGVRVREAAQIGQEIRKGIAAGRATLIHVPIKGGNNLP
                     QL"
     gene            17665..17973
                     /locus_tag="VFA54_12800"
     CDS             17665..17973
                     /locus_tag="VFA54_12800"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HZS51737.1"
                     /translation="MNPPAPPAVNKRGKTLMLWLLFFFFLDVCTTIVGLSEGFRELNP
                     VVSSALHLTGLQGLVLTKLMALSLAGYFLYSGRLLLLRKVTVLMGLVVGWNLFWLVAR
                     "
     gene            complement(18114..18725)
                     /locus_tag="VFA54_12805"
     CDS             complement(18114..18725)
                     /locus_tag="VFA54_12805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HZS51738.1"
                     /translation="MGTTRREMLYQIAASVTAAGAGALPAAGQRQQGLPPEHADHNAP
                     SPVRRGPRKAFTEHEFRTLQNLSEWIIPSDERSKGGIEAGTAEFIDVMAAGDQELQFA
                     FTGGIAWLDQQMESRHGKPFIACSQAQQKEMLDQIAYRDKAPPELGPGVKFFALMRAW
                     TVDAFYSSKVGIEDLGYVGNTALPEFNGCPDRVVKSLLEKSPV"
     gene            complement(18727..>19223)
                     /locus_tag="VFA54_12810"
     CDS             complement(18727..>19223)
                     /locus_tag="VFA54_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZS51739.1"
                     /translation="GVGFSGRGEMIPNEHCYCEIDPNVVDRFGIPVLRFHWKWGEPEL
                     KMALHMRRTFEEIIQTLGGTVVPQPESGGGRRGEGHEFRLGSDINPGGSIIHEIGCTR
                     MGSDPKTSVLNQYCQAHEVKNLFVADGGPFVSNPDKNPTLTILALSWRTSDYILDQAK
                     KGNL"
BASE COUNT         3564 a         5756 c         5998 g         3905 t
ORIGIN      
        1 gcgtgagtat ggcagtgcgc ccgatagtga ggatgcgatc gccatgccga tccaccgctt
       61 tcttcaattc cagctcgccg catgtgtact tcatgggcgg cagaaacctg ccgtcgggca
      121 gcgcttccca tccttcggcg cggccgctca cgccgatgaa atcttccacc aggtcgtagt
      181 aaggcgcgag atcctcgtag cggaaaggcc agttgacgcc gtagccgtca tgcgacgcgg
      241 cctgaaaatc gtaattgctg tagcggtacg actggcggcc ccacgtgatc gagcgccctc
      301 cgacctgccg cgaccgcacc cagttgaacg gcttgtccgg cggagtcgtg taaggaacat
      361 ccgcgtcatt cacgaacagg tgcgttgtgt actcgctcga gaaacccgcg gcgctgccct
      421 ggcgcacttc cagatccatc ggtcggccga agccgcgaaa cggcatatca tagggccagg
      481 cgtgctcaag aaaatctttg gccgggtcca gcttgcgtcc cgcttcgagc agcaggactt
      541 ccatgccccc ttcggtgagc tgcttggccg cccagccgcc ggtggctccc gagccgacca
      601 cgatcgcgtc atagattttc actggcataa aagcgacttc atccgccgcg cctttgccgg
      661 gcgcagccgg cgcatggcga tttcaagcgg atcgtccgat tgcagggcga gccgcatacc
      721 tcagcccctt tgctccggcg ctgcgcaccc cggaaagtcc acctgcgccg tatttcctat
      781 gtattgcaga tccttatgga tcccgacggg cgatgtgtag tacccgttga tcgtcatcag
      841 cttcagcgcc gcaaagaagc gttccagatc gtttgccggc ttgttctcat tggcggccat
      901 cgcgcgcagg atttgatctt gctgcccggc attgcagtcg agaaaccgat tccggaagcg
      961 gttcttggcc tcgctttcga cggcttgaag acccgagatc cattgctgct gcacgtcttt
     1021 gcggctattg gcgaccataa gatcgataaa gaggcttacc ttcgcctcgc gggctcccgg
     1081 agagcgcccg tccgcaggga tgatcaattc ggacagttga tcgagcagcg cattctgagc
     1141 cggggtgaag aactgaagct tataggcgcc gggcgcgcgg gcaatcgttt ccaggtgatg
     1201 ctgggcctcg gcaagccgct cggccagaac ccccgccgca atcagtttca gggccgcacg
     1261 ccgatcgata tttgacatcc gccaatttcc tttggacgcg attgtagcac tctacagcga
     1321 gtacagccta cccccgtgct gtccgatcag aatctgaaaa atccggtgtc cgatcaactc
     1381 atgcaaagtc agcagctcgc ggtttgcctc cacgatattt ccaaccggcc cggagccgtt
     1441 tccgcccgcc cggagcgcgg cggcagcctc gccgaggatc ggatcggaaa ttacctcccc
     1501 ggccatttcc caaagggtga tgaagcggtc cacagcggcc ggaaaactga catgaaatac
     1561 gccccagttg gacgttgtgc gcggcggctt gtagtccagg ttgaacgggc cattgtaatt
     1621 atgcaggcga agcagcacga gcacgttcag ccagtcgagg ggattgacca aaccgacggc
     1681 gatgtccacg tcgtgcttga gccggtagcc atcgttgatg tcgaaatgcc agagcttccc
     1741 cgcgagcagg gcccgggcca gagcagcccg cggcgctccg ccccacatca actcgtgcag
     1801 atattcgggg ttcacgccca ccatctcggg atgggtcagc aagccgctcg ctatgaaggc
     1861 caacatggcg tccgtagtgg gtagaagaat ttcggcctgc ggctcaaaag gcttggcctc
     1921 gaggcaatgc cggagcgtgg gccggccgtc ccttcgcgca acttccctgt cgcgcgcgca
     1981 agcggcgttg atgccgtccg cgaaccagcg cagcgtccga gtctcttcga tcgcgccctg
     2041 cacgaggtag cccagcgtac ccggccagta gaccacgaac tgggctccca gccggcgccc
     2101 gatctcgacc gtgttcgcca ggcgctccgc cgcatattgg cgcacctccg gggcctcggc
     2161 agccgaactg gcggcccata ccgggtgatg gaacgtctcc gtagtcacca tcgagcagcg
     2221 caccccatgc tcgtcgagcg cgcgcttgat atcatcgacg atttcccact ggcgctcagt
     2281 aagcagagtg ccggcgggaa tcacgtccgt atcgtgcgta ctccagtagc cgatgccgat
     2341 gcgggaatac tcgcgaatca catcgcggta agtgatcggg tcccgggttg gcgcgtggaa
     2401 cagcgcctgg ccctgatagg ccgcggccca ctgcggcccg ctgataaaat gcctgcgccg
     2461 gacctccggc gaatagctgc ctccgcaggc ggccatgagg cgttcgacca gcgcgcgttg
     2521 cgtttcgctc atgtcatcag ttgtaacgca cgggcgggtt aaagaaaagg agcagaagat
     2581 aaacggtcat gcgagaagtg ctggagcgcc ggttccgctt gcgggaacac gaaaccacca
     2641 tcagccggga gatcgtaggc ggcgtcacca cgtttctgac gatgtcctac atcctttttg
     2701 tccagcccag cctgcttagc caggcgggca tggatttcca ggccgtgctg gtggctacct
     2761 gcgcggcgtc ggcggctgcg acgcttctga tggctttagc cgcgaattac ccggttgcgc
     2821 tggcccccgg aatgggcgag aacttctttt ttgtatacac gctctgcggt tcctaccgtc
     2881 tgacgtggca gcaagctttg gcggcgatct tcaccgcggg agcggcattc atggctctcg
     2941 ccgccttcgg tcttcgcagc aggatcgtca acgcgattcc caatgcgctg aagaccggta
     3001 ttgcagccgg tatcggcctg ttcatcagcc tgatcggcat gcaatacggg cgcctggtcg
     3061 tggcgaatcc ggctacactg ctgcaactcg gcgatctccg cagcccgatc gctctcatta
     3121 ccatggccgg cctgctgctc actctgggcc tcctggcttt cggcgtacgg ggggccatct
     3181 tgctagggat tttgggatcc accgcggtag cggcgggctt tgggcgcgtg cattattccg
     3241 gcatcgtcgc ggcgcccgcc ggactgagcg ccacgctctg gaagatggat ttcgccggcc
     3301 tgttccatcg cgacccggcc acagtcgccg tagtggtctt cgtgctgttt tttctggcct
     3361 tgttcgatac agtgggaacc ctggttggcg ttggaaggca ggcgggtctg atgagaaacg
     3421 gcgcgctgcc gagagccgag cgggcattgt tttccgacgc caccggcacc gtcgtgggag
     3481 cggcgctcgg gacttccacc gtgacttgct acatcgagtc ggcggccggg gtctctgacg
     3541 gcgcgcgaac cggactggcc agcatgacaa cgggaacgct gctgttgatc agcatgctgt
     3601 tcgctcctct ggcgcgcatg atcgggggcg gcgttccagt gccgagcgcg caaggcggcg
     3661 gagtcgccta tccgaccctt gcgcccgcgc tcatcctggt gggggccatg atgatgcgtt
     3721 gcgcgcgcga catggattgg gaagacccga cggaatcggt ccccggcttt ttgaccatga
     3781 tcctgatccc gttttcgttt agtatcgccg ccggcgtggg agcgggcttc gtgctatacg
     3841 ccgcggccaa gctgctcgcg gggcggccga aagaatgccc ggcggtggtt tacgtatttg
     3901 cggccttgta cgtcatccaa tatgccgtgg cgcggcaatg aggcaatcga ggcagacgcc
     3961 cgcgttttta ctcggccgcg aacggcccct tacgccaagg cgtccctgag cacttccgcg
     4021 gcatggcgcg gagtgcgccg tgtgaagtgc gagatctgct ggcggcagct tacccctgta
     4081 atcacaatct cggtgtcggg gctggccgcg ttgacagcgg gaatcaggcg cgtctcagcg
     4141 atgatcctgc tgatgtcgta atgctccttc tcaaatccga acgatcccgc catgccgcag
     4201 cagccggagt tgatttccga aacttcgtag ccggcgggaa agcgcagggc ctggagagtc
     4261 gatgccgtgc caatgtgagc tttttgatgg caatggccgt gcaaaaggat ctttttccgc
     4321 tcctgcctcc attgcaagcc gagtttaccc tcgtcttcca gtttgcacag gtactcctcg
     4381 aataagtaag actgccgggc gacgcgctcg gcggcgtgtc cctgaagcag gtccaaatat
     4441 tcgtcccgca atgtcagcag gcagctcggc tcgcagccga cgacgacggc tcccttttcc
     4501 accagaggaa ccaacgcctc cacattgcgc cgggcggcgc ggcgggcgtc ttccagcaga
     4561 cctttggaga tcatgggacg cccgcagcaa tcgtgcttcg ccaatattac ttcaaatccg
     4621 gcccgctcca gcagttcggt cgcagcccgg cccacctgcg ggtagttata actgaggaaa
     4681 caatcgtcga agagtacgac cgcacccctc gcagcgcggc cattggccct gccattgccg
     4741 ccggcccgct tcgcaaacca gctttggaac gtctcgctgg cgaaagccgg aaattcgcgg
     4801 cgggcgtcga tgccgaaggc catttcggcc agattctttg cccagcgccg gctcatcatg
     4861 taattggcga cgcccgccat gcggcttccc cagcggttca gtttgccgat gttggcgaag
     4921 agcagagcgc gttcgggcac gccgtgtttg gcgtagtact gggcgaggaa ctcgtatttg
     4981 attttggcca ggtctacatt ggacgggcac tcgcgcttgc aacccttgca ctccagacaa
     5041 agatcgagcg tctcgtacat ccggcgggac gtcagctctt cggcgggcaa gcgcccggaa
     5101 agcgcggcgc gcaaagcgtt cgcccggccg cgcgtcgaat gctcctcttc gcgcgtcgca
     5161 tggaaggacg ggcacatcgt gccctcgagc gtcttgcggc agatgcccat gccgttgcac
     5221 atttcaaccg cgccggcgaa ccctccttcc cgtgagaaat ccagatgcgt ctggatctgg
     5281 atggtctggt acgttttgcc gtaacgcaga tcctgcgtca tttcaggcgc gttcacaacc
     5341 ttgcccgggt tcatgatgtt ctgcgggtcg aagatcgcct tgagcttctg aaacgcctcg
     5401 tagatcttgg ggccgaacag ccgggcgttg aaatggcccc gcgccagccc gtcgccgtgc
     5461 tcgccgctca tcgagccgcc gcactcgacg acaagctgga agacctcttc cgcgatgtcg
     5521 cgcattttgt acatttccga ctcttgcttc aagtcgatca agggccggat gtgcaggcag
     5581 cctaccgacg cgtggccata ataaccgccg gtggtatcgt gatcggcgag aatctggcgg
     5641 aagcggcgta caaactcggg cagccgctcg accggcaccg ccgggtcttc cacaaaggcg
     5701 atcggcttcc gatcgcccgg cacggacata agaagaccga gggaatcctt acgcaccttc
     5761 cagatgctgg cttgcctggc cggatcgacc acggggagat ggccgaagcc aatgcgcttt
     5821 cgtttgagcc gggcatcgag ttcctcgact ttcgagcgca cctccgcctc ggtgtcgcca
     5881 tagaattcga cgatcagcag ggcgccggga tcgccctgca caaaatccat ctgcttggcg
     5941 aactgcggcg tcgcgcgagc ccgctcgatg atcaggcgat ccaacagttc cgtggccacg
     6001 ggactggtga ccgtcatctc atctgtggct tcgacggcat cgaccaggtc atcgaagagc
     6061 acaatctcga tggctttttt ttggggcagg ggcacgatgc gcactttcgc ttcgctggcg
     6121 acggccagcg agccttccgc gccgacgatg gctttgcaaa ggttgaacag gccggggcgc
     6181 gtggcgaagc cggcgccgaa gatgcccggt ctctccgctc cgggagcggg aacgaattcg
     6241 tccagattgt agccgctgac gcgccggatg atcttggggt accgcgcgaa tatttccgag
     6301 cagtaggctg ctcccagagc gcgtacttct cggtacagcc ggttttccag tgcgtcgccg
     6361 cgcgacaact gctcgagccg ttcgggcgtc acttcccgga agcgcgcttc ggagccgtcg
     6421 gatagaacaa catcgagctc cagcgtgtga tcgatggtct tgccgtaaag aatggaatgc
     6481 gagccggccg agttgttccc gatcatccct ccgatggtgg ctcggttcga ggaagaagtg
     6541 tcggggccga agccgaatcc gtagggccgc aagtacgcgt tcaattgatc ctggaccacg
     6601 ccgggctgca cgcggaccca ccgctcttcg gtgttcacct ccagcacgtt gcgcatatat
     6661 tttgcgaaat cgatctggat ggcatggccg accgtctggc cggaaagact cgtaccgccg
     6721 ccgcgcggga gaatcggaat gccataacgg gcggccacac gcaccgccgc agccacgtca
     6781 tcccgatgcc ggggcacaac gacgccaatc ggctcgatct ggtagatgga ggcgtctgtc
     6841 gagtaaagga ccttggaata ggcgtcgaag cgtacttcac cttcgatctc tttccgcagt
     6901 tcggcctcga ggtccgggct cgggtgtttc atacggcgtc tccgggcggg agccagaagg
     6961 ccgggccggc tgccgtgatt gtaatcgcgc gtctgtgcat gtgtttgcgg gcgtgcgggt
     7021 aatttggaag gaagactccg cagccccgcc tgacctcttt cagtttaata atcgcttgcg
     7081 ccgcaacgca atcagtataa tgaagagaat gattctgcgg cgattatgcg cattgctggc
     7141 gagcttttgc tccttgctgt gcgcggctgg ggaactgtcc aatcgcccgg cgccggattt
     7201 tcacctcagc gattcccagg gccggatggt gagcctgagc tccttcaagg ggaaggtttt
     7261 gctcgtcgag ttcctgtcta ctacctgtcc ccattgccag aagttcgctc cggtgctgga
     7321 ttcggtctac acccggctga aagggcgcgt ggcggtggta gggatctcga cctttcccga
     7381 caccgccgag accgtggccg agttcgtgaa aacgtataaa attagctatc cggttttagt
     7441 ggacccaacc aacaaggcgg cgatcgatta cctaaagccc gacccgaatc acggcttttc
     7501 catcccccac gtcttcgtgg ttgaccagtc cggttacatt cgggatgatt ttgtgcaaaa
     7561 cccttctaat gcggaattgt tcactcccga gggcctgact cgcctgttgc gggcgtattt
     7621 gacgagcaag tgagccaggt accaagagtt tgtcttaaga ggtacctgtc ttgcctctaa
     7681 acgattgtcc tattgtgttt tgtcgcatcg ctgttacaat cgcaggggat tttgcaaaaa
     7741 aagcttggca ggcattcatc ggagagaaac aacaagggga tagaaatcaa aggtgaaatg
     7801 gtccgtgaag gaacaacgac gctcccagcg ctttcagctc accctcccgc tgaccctgat
     7861 tcggggcagc cggggcgaga tcaaccggat tggcgagacc aggaacctga gttccaccgg
     7921 cgtttacttc gtggtcggcg aagcggtgga actcggaagc ctgctggagt tcgtggtgac
     7981 tcttccggat gaaatctcaa tcgccggtcc ggttcggctg ctttgcaagg gcaaggtgac
     8041 gcgtgtcgag cagcaagagg cgagtctggt cggagtggct gcaacgatcg agcgttacga
     8101 gttcgtccgt gataacgtga actaatccaa gaccaaagct ttcagtcaca taggccgggc
     8161 ttcccggctt tcataacttt ttgacaactt ccggggctgc cgctcttgct tatgtatatg
     8221 aatttgatat agtaggattc cgttccatac aagttcgtcc acgcggacgc tctagctttc
     8281 tacattccgg aaaggaaggt gcctggtgag caaaatagac tctgatgacc atggcaaaac
     8341 gaaacagccc ggcaagaaag gattctcgcg gcgctcattc ctgcaagggg ttgggattgg
     8401 aggcggcgcg gtagccagct tgcccaccgg cttgctggtg gagcccgagg ttgcgagcgc
     8461 ggcccccgtc atggggcccg ggccggttcg ggtttcacta cgtattaatg gtaaggttca
     8521 caacctgaat atcgagcccc gggtcacact gttggatgcg ctgcgaaatc atctggatat
     8581 gaccggggcg aagaaagtct gcgaccgggg cacttgtgga gcttgcactg tgattctgaa
     8641 cgggcgcgca gtttattctt gcagcgtcct ggctattgaa gcgcagggca aagagatcca
     8701 gaccatcgag ggaatcggct ctctctcgaa actgcatccg gtttccgccg cgttcgtgga
     8761 gaacgacgcc cagcagtgcg ggttctgcac accgggattt gtgatggcgg tcaaggcttt
     8821 cctggacagg catcccaatc ccaccatgga ccaggtgaag aggggcctgg gcggcaactt
     8881 atgccggtgc ggcacctatg tgggcgtgcg gcaagcggcc ctggacgcag cgaaggagat
     8941 gaaaggaggg cgtcgtgcct agtaaatatg attggcctcc cgctgaaaaa cgcagcctga
     9001 tcggcaaacg ggtgaaccgg ctggacggcc cgcacaagtc aagcggggtt gccagatatg
     9061 cctacgacac caatccgccg gggttgctga cggcgaagat gctgaccagc ccttacgccc
     9121 acgcgaaagt gacgcgcatc gacaccagcg aagcggaacg gctgccgggc gtgcgcgccg
     9181 tcgaagtcat cgcgcccgcg ggaaccgagc tccagtggga agggcaggag atcgcggccg
     9241 tagcggccga taccgaagag ctggcgcgcg acggcgtccg caaaatcaag gtcgaatatg
     9301 aagtgctgcc gcacctggtg cacgagtacg atttgaccaa ggcgggtaac cgggccaagc
     9361 cgtcggcgga ggaaaccaag gggaatcccg accaggcttt ctccagcgcc gatgttgttc
     9421 acgaaggcga gtatggcatc aacacggaat gccactgctg cctcgaggct cacggccagg
     9481 ttgtggagtg gaacggcgac aacgtgacag tcaccgcttc cacgcaggcc gtcggccggc
     9541 tcggcggcga tttggcgaaa gtactcgctt cttcgccggg aacggagaac atcaagccct
     9601 ctaatattcg cgtcatcacc cagtacatgg gcggcggctt cgggtcgaag ttctcgatcg
     9661 acacttgggg catcgcttgc accaaactgg cgcggcgcac aggcaagcca gtgaagctga
     9721 tgctcgagcg ggatcaggaa ctgcaaatgg ccggcatgcg tccgtccgat ttcgcgaaaa
     9781 tcaaagttgg cgcgaagaaa gacggaaccg tcgtagcctg ggagtcggag tcctggtcca
     9841 ctggcggcat ggctggcggg ggcgctcctc cactgccata cattttcgct gacattccca
     9901 accagaagaa gcggcacatt gcggtttcga ctaataccgg gccggcgcgg gcctggcggg
     9961 cgccgaatca cccgcaagcg gccgttctca cgatgaacgc cctcgacgat ctggcggcca
    10021 agctaaacat ggatccggtc gaggtgttca tgaagaacct gcactacacg ccgcgtccgg
    10081 acgtttacga ggccgagctc aagaaggcta tggagctgat ggactggaag gcgaagtggc
    10141 atccgcgcgg ccagggcgga aacggccata tcaagagcgg tgtgggcgtt tcgattcaca
    10201 cctggggcgg ccggccgcac gacagcgatt gccgggcgac gatcaacccg gacggcagtt
    10261 gcaacgtggc ccttgggagc caggatctcg gggtgggctg ccggacggtg attggcatcg
    10321 tggctgcgga gaccttcggg ctgccgctga gcgccgtcga ggttctgatc ggcgacagca
    10381 attatccgcc ttcaggcgcc tccggcgggt ccacaacgac gggcggcgtt tcggcttcca
    10441 cgcgccgcgc ctgcatcaag gcgctcgaca agctgttcga ggctgtggct ccttcgctgg
    10501 ggaccacgcc ggataagctg gtggctcggg acggccgcat tctcgtcgag ggcgacccga
    10561 gcaagagcct gacatggaag gcggcctgcg ccaagctcgg cagcaagccg atttcagaaa
    10621 ttggccggca gccggcgcgc ggcgagggca acctgaacga cagcaacgtg ggcggcgtcg
    10681 gaatggccga ggtagaggtg gacaccgaga ccggcatcat cacgatgaag aaatacgtgg
    10741 cggtgcagga ttgcgggctg gtcatctcgc tgaagacggc cgagagccag gtttttggcg
    10801 ctgcgatcat gggcatctgc tcggcgcttt acgaagagcg gatctacgat cagcaggttg
    10861 gcaagatgct caatgccaac atggagttct acaagctgag cggcattgga gacatcggcg
    10921 acatcgtcgt gcatatggtg cagggtccct atgacagccg cggcgtcatc ggtctcggtg
    10981 agccgccggc cgtttcgccg cctgcggcca tttccaatgc ggtggctaac gccatcggtg
    11041 ttcgcgtgca gcctctgccg atgacaccgg acaaagtcct tgctgctctg gcgaggggag
    11101 ggagggcata atgcaagctt tcgaatacgc cagtcccaag acgacgaagg aagcccttgg
    11161 cctgctcggc tcccagtggg gcgagaccga cattctggca gggggcaccg accttctcgc
    11221 tctgatgaag gattacatcc acacgcccag gcgggtggtc agcctgggcg gtatcaaaga
    11281 gctgaaaacg atcaagtcat ccggcgacgg cttgcatatc ggcgccatgg tgaccttcgc
    11341 ggagctgatt gaaaactcga caatcagcaa ggaatatccg acgctgaacc aagcggccca
    11401 cggcgtctcg agccagcaga tccgggcgat ggggactgtg ggcggcgatc tgtgccagcg
    11461 gccgcggtgc tggtatttcc gcaacggctt cgggctgctc ggccaggatg gcaagggcaa
    11521 cccgctggtc ccgaatggag aaaaccggta tcacgcgatt ctcggcaaca gcgggccggc
    11581 gaagttcgtc agcgcgtcga gcctcggccc gccgctgatt tcgctggggg cgaaggtcgt
    11641 gatcgcgtcc tcgtcgggca accgcacggt agatgtggcg aagttttttg tcacgccgga
    11701 aaatgcaaac atgcgcgaag tggatctcaa gcccaacgag attttgacgg agatcatcat
    11761 cccgccgcat ggaggcgcaa agacggcgac gtacgaagtc cgccagaaaa tggcgctcga
    11821 ttggcctttg acgacggctt cggtcgcgct caagatgagc ggctccacgg tccagagcgc
    11881 cgtggtcgtc cttggccatg tggcccctac gccgtggaat tcgcccgaag cggcgcaggc
    11941 gatcacgggc aaatcggtga ccgatgcgac cgcccaggcg gcggctgacg cggctctggc
    12001 tcgcgctacg ccgctgagca agaacgcata caaagtgcaa ttggccaaag tggctgtgaa
    12061 gcgcgcattg ctcctggcgg caaccgggag ggcttaaatt atggagaatg cgggactgaa
    12121 ggttcttgcc gagcgctgcg gcaatctacg gtggaaagga ttgtttatcg acgccgaatg
    12181 ggacccgaca gtccagcagt ctaacgatcg gatttactgg tgcgtccaga cccagaatcc
    12241 cctgggtccg gacggccagg tggtcgatga agatgtctgt aatcccaatc gttcctgcta
    12301 cgaggctctg tagcgggaga ggtggtatac taaaaaacga gcggttcgtc aaactggagg
    12361 tctaggatga gaaaggttct actaactgca acactgctgc tggcctcagc ggcgatgctg
    12421 tcggctgccg agatccgcgg cagctacatc gaagcccgca atgccgacat ttgggtcgcg
    12481 cagtgctttg ctaattcgga agtagggatc gtcggcgatc tggcccttat gggctggaag
    12541 atcgacaaag ggtcgtggaa caatgtatcg ctcgatggta tgggcgtcgt ggccgtcgtg
    12601 agggccaaga gcaccctggg cgacacattc cacagcgcct atccgaccaa ggccatcctg
    12661 atcgtggatg agcgcgcgaa tatggagcag cgccaggccc tccagagctt cgtccagaag
    12721 atggcgggcg atctggtgca gaccgtggtg cgcgtggaat cggcgccgat cgagttcaat
    12781 atcggcggga gcatccactc cgggtcggcg cagctcgtcg ccggcaacct ggccaggatc
    12841 cagacccgcg ctctgcggga gggtgatgcg gtttgccaca atgcgatcac ctactatccg
    12901 ccgctgagcg ctaacctgga gcattcgatg ccggcccaca ctgttgcgaa tacgttctcg
    12961 gggcagggct tgaacgttgt ttggtcgaat cccgacaaaa acagcgcctt cctgggcagc
    13021 ttcgcgacag aatccgacta atttcttctc tgcttgattc cttctcgcag ggcgggcgcg
    13081 aagcccgccc tgcgtcttcg cgtgctataa ttcgactgtc atgccagctc tgaaatcctt
    13141 tgccccgcta ttcttgttcc ttatcttgtt ggcgccggct agagcccaat atacggtagc
    13201 gccgatcggc gcttgtgacg cctccggcgt ctccgatgcg gtgaagaatg ttttggagcc
    13261 caatggctac caagtgaaag acgcgagcgg cgccccttat gccgaagtct ggctccgaaa
    13321 gggcattcct tccgacaaaa atgctccttc ggcgggcggc gccgacttta cctcgatccc
    13381 cctcagcacc ctgattgggg tcatccgatt cgacaaggat accaacgatt tccgcggcaa
    13441 caaggtccgg gccggtgtgt acactatgcg ttatcaactc cagccggagg acggcaatca
    13501 ccagggcgca tctccgcgcc gcgaccatct ggtgctttcc ctggcggcgg acgacaccga
    13561 ccccaatgcc aatcccaatt tcgatgcctt gattaagcag agtgaaaagg ccagcggcat
    13621 caaccatccc ttagtgcttt tcctggcgct gcccgacccc aacgctaagt ttccgagcat
    13681 tgagcacgtc agttcgcgcg acatgctgga tgtgcaaaac gggtctctcc aactcggaat
    13741 caccgtagca ggcaaagcag aagaataatc ccaacccgac acgacctggg ccgatgcacc
    13801 tcccgcctat ccatcttttc agcactcttc gaggccggct gctttcggag tatcattccg
    13861 agtaacggct tgcagcggct tcaaggctcc cggcaggcgt ctacacggcc cgtccggcgg
    13921 ccctatgtgc gcgccagaga tcctcggcgc acaggctgtt tcaggacggc aaaggagtag
    13981 tgaataatcg gtcatgcggg cgctttcccg gctggatatc gcgctgctca gcttgtgtct
    14041 ggcgctcttc ttgtttttcg gcgccgcctg gatctcattg ccgggacctc aacaggatga
    14101 attgttgcac atctccgtcc tgattccccg gttgcgaggc ttggaaacct tctcgctaaa
    14161 gacgaagcat catcagtatc ccttgatgat gatgccctac gtaggagcgc tcaagggctg
    14221 gctgctgtgg ctgtggtttc gcgtagttcc gatgggagtt gtggggtacc ggggcttcgg
    14281 cattgccgcg ggagccgtta cggtgtggct catcttctgg ttcgtgcgcc ggcactacgg
    14341 cgatacagta tcactgctta ccaccggcct gattgcgacc gacccggtct ttgtgcaggg
    14401 aattcgcttg gactatgggc ccgtcgctct aatgcacctg ttgaaggtgg gcggattatg
    14461 cctgctttcg atctggctgg cgagccggtc tcgctgggct gcggcggcag gcatgtttct
    14521 gtttggcctg gctttgtggg acaaagccaa cttcgcctgg tttctggccg gcttgagtgt
    14581 gacgatggcg attctgtttt cgcgtgaagc aattgcccgg ctgcgcgccc agccgcaact
    14641 gctcctgatc gcctgcgccg cgtttctgat cggcgcggct ccgctgcttg tctataactg
    14701 gaagcgccac ggggaaacct ggagggagcg cggccggttc gaggtccggt ggttcaagct
    14761 gattgaggct tccaaaacct tcaacggccg tttcatgctg gatatgaccg ccggcgatca
    14821 gtatgacaac tcccctcctg ccgccgacgt gtttctgcct gggctctccc agtggatgta
    14881 ccgtctcggg cgccttcgcc agaccatcac gcttccgctg ctggcggcgg ctctggcgct
    14941 tttgcccttg aatctgcggc tcacgcgttc gcggagatcg ctgctgtttc cgctgctgct
    15001 gtcggcgctt acttacgcct gcatgttctt ttctcgtgac ggcggcgcga gcgcacacca
    15061 cgtcatcatg atccagccgt ttccgatgct tttcctcgcc gtctcgctct ggacgccggc
    15121 ggaaaggcgg ccatcgcttg cggcgcgcgc cgccgcaacg gttgtcgtct tggccgccat
    15181 cgccgtgaat atcagcctga acgcgcggca ccttgccgtg tatacgcgga cgggcgggac
    15241 ccgagcgttc agcgacgcca tttaccggct ggttccttac ctggcgcgga actccgggtg
    15301 gaggctatac gcgatggact ggggattctc caatccggtt gcgtttctgg gccggcgctg
    15361 gaatctccgc gtggatgact tctttttcgc cgtcaacggc gacagcgaga gcagccggat
    15421 caacgaagaa cagctagccc ggctgatgcg cgatcccggc aatgtatttt tgctgcatag
    15481 cccgccgagg gtttttttcc ccaacccggg ggaaaagttt ttcgcgctgg ccagcagcgg
    15541 gataccgatg cgcgagatag cggagttccg cgaacggtcc ggcgaagtcg tctaccagat
    15601 ttaccgctgc gaggagcccg attcgccggg caaggctctt tctgaagtcg aagtccggtt
    15661 tactccccag caggttgttc cccggcagca atacacgatt gaggtccggg aaatccacga
    15721 ttcctggatc gatctggttt atgatgtgaa tcagaccagg ggtgcagcca atcgattctg
    15781 ctatctcgac gcccagggac gggcgaaaat gacggttcca gcaggatacc cggcggcgac
    15841 agtgcgaatt ctgcggatcc ggcccagtgg gggcgagtgg cgtcccgccc gcggctcgat
    15901 aaccgtcgtt ggaggaacgc aaaacccatg accggcgcgg aactgttcgt agagcggctg
    15961 aaagaccacg gcgtgcgatg ggtctccacg ctttgcgggc atggcctgga tccactgttt
    16021 gacgcgtgcc ggcgagcggg tttgccgctg atcgatacgc gcaacgagca ggccgcttcc
    16081 tacatggctg atgcctgggg aaagctgacg cgcgcgcccg gcgtggttgc cgcatcgagc
    16141 ggagtggctc acgccaatgc gctggctgga gtcgtcaacg cgtatttcga cggctcgccc
    16201 atgcttttgg tgaccggtgc gcacgccgtg gccaccatgg ggcatggtca tttccaagac
    16261 cttgatcagg tcgctctggc cgcgccggtc tgcaaatccg cccggctgat cgatcggccg
    16321 gaaacgattg ctcctctgct tgaagaggcg ctcttggcgg cggtcgcacc ccggccgggg
    16381 ccggtgcatt tgactttccc catggacgtc cagcgggcag aggcgccctc cggcgagcgg
    16441 atagcaaggc ggctgcggga gccgcagagc cgttcgcccg cgccgcttta tactttgccg
    16501 gcgtcttaca gccgtcccct ccttatcgcc ggcagcggcg ttttctatgc gcaaaaagaa
    16561 caggcgctgg cgcgtttctg tgaaaagtct gccgtgccgg tggtgacgcc gatttgggac
    16621 cgtggatgcg tcaaccaggc gattccccag tttatgggcg tgatcggcgc tgctagcggc
    16681 gggccgcgat tgctgccgga tgcggatctc attctgttgt gcggcgctgc ggcggactac
    16741 cgggtgggtt acttgcaggc gccggcgatt catcccgccg ctcacgtcgt gaagatcgag
    16801 ccacagcact tggagacact ggagctggcg cctcagccgg cttggctcgc ggaagcccag
    16861 aggaggcggg acgagtttcg gcgtgccttc gcatcggttc cccggcagcg ggccggcatt
    16921 cacgcgctgg atatcattga ggccctgcgc ggcgtcctca ccgaagagac cgtgctcctg
    16981 atcgacggcg gcagcatcgg acagtggtgt catcagctcc tgtgcgaccg ctaccccggc
    17041 cactggctca cctgcggcgc aagcgggacc gtgggctggg gcttgggcgg ggccatggcg
    17101 gcgcggctcc gtttcccgga ccgtccggtg attctcgtct ccggcgacgg cgccgcgacc
    17161 ttcaccattg gggaactcga gtgcgccgtt cgccagcgct tgcccttcgt tctcgtcgta
    17221 gcggatgacc aagcctgggg cattacccgg acaggacacg agcgggaatt tggacagccc
    17281 atcgccagcg agctcgggcc gatcgacttc gcaacgctgg ctgaatcatt gggggcgcgg
    17341 ggagtgcggg tccgcgaggc ggcgcaaatt gggcaagaga tacggaaggg cattgccgcc
    17401 ggccgcgcta ctctaattca cgttcccatc aagggcggaa acaacctgcc ccagctttag
    17461 caggctttca taaaatgaac ggctccggcg catctttggc gcatcactaa ggccggacgt
    17521 ggctttcgga attttgtgaa gacacgtcca ttgcggcacg ggtttgtatg ctctttccct
    17581 catatgtcag tactaggacc aaatttccgt tgcaagccag agtctcttgc gttacgttga
    17641 gggcgtatct cgaacagtct acttatgaac cctccggctc ctccggcagt gaacaagcgc
    17701 ggcaagactc tgatgctgtg gttgctgttc ttcttcttcc tggacgtctg cacgacaatc
    17761 gtcggactca gtgaaggttt ccgcgagttg aatcccgtgg tctcatcagc attacacttg
    17821 acgggattgc agggactcgt gttgacgaaa ctgatggcgc tttcgctcgc cggttatttt
    17881 ctttacagcg ggcgattgct gttgcttcgg aaagtcaccg tgctgatggg cctggtcgtc
    17941 ggttggaatc ttttttggct ggtcgcaagg taggcagttc cctcctgtgg cgcaaaggca
    18001 atgtggcaga cgcggcgctc tcaggttcta ggaatgaatc tgatcggatt ctcgtttctg
    18061 ccaaacggag gcgcggcgct ggttccagac agggaaacga gccgggggcc ggctcacacc
    18121 ggagattttt ccaagagact ctttactacc ctgtcaggac agccgttgaa ttccggcagc
    18181 gccgtgttgc ccacgtatcc aaggtcttcg atacccactt tacttgagta gaaggcgtcc
    18241 actgtccaag cgcgcatcag cgcgaaaaat ttcacgcccg ggcccaactc cggcggcgcc
    18301 ttgtcgcggt aggcgatctg gtcgagcatt tctttttgct gagcctggct gcaagcaatg
    18361 aacggcttgc cgtggcgcga ctccatctgc tgatcgagcc aggcgatccc tccggtaaag
    18421 gcgaactgaa gttcctggtc gccggcggcc atcacatcaa tgaactccgc tgtcccggcc
    18481 tcgatgccgc cctttgagcg ctcgtcggaa gggatgatcc actcagaaag gttctggagg
    18541 gttctgaatt catgctctgt aaaggccttg cgcgggcctc gccgaaccgg gctgggagcg
    18601 ttgtggtcgg cgtgctccgg cggaagcccc tgctggcgct gtccggcggc cggcagcgct
    18661 ccggccccgg cggcagtcac cgaggccgct atctgataga gcatttcacg gcgtgtggtt
    18721 cccatgtcaa agatttcctt tcttcgcctg atccaaaata tagtctgacg tccgccacga
    18781 gagggccaga atggtcaggg tcgggttctt atcagggttg ctgacaaacg gcccgccgtc
    18841 ggcaacgaag aggtttttca cctcgtgggc ctggcagtat tggtttagca cggatgtctt
    18901 tggatcgctg cccatgcgcg tgcagccgat ctcgtgaatg atggatccgc cgggattgat
    18961 atcggatccc agccggaatt cgtggccttc gccgcggcgg cctccgccgg attccggctg
    19021 cggcacgact gtcccgccta gggtctgaat gatctcttcg aacgtccggc gcatgtgcaa
    19081 cgccatcttc agctccggtt ccccccattt ccagtggaag cgcaaaaccg gaatgccgaa
    19141 gcggtccaca acattgggat cgatctcgca atagcaatgt tcgttaggga tcatctcgcc
    19201 ccgtccggag aagcccacgc cgg
//
LOCUS       DASREK010000241        20415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1594783, whole genome shotgun sequence.
ACCESSION   DASREK010000241 DASREK010000000
VERSION     DASREK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 20415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20415
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594783"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFA54_12815"
     CDS             <1..405
                     /locus_tag="VFA54_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51740.1"
                     /translation="NFEFVVPILASVRWSGKIGHGQGLGDEFFRFGQAFNNRGLPIRT
                     TAGWTELGYYGSKWSVAGGWGDDDPNDTDLRSISNNNGNYTHNQRIFVNALYDLFAHV
                     KVGGEYNYLRTNWTNGDLFKAHQVMGVVFYSF"
     gene            426..1700
                     /gene="urtA"
                     /locus_tag="VFA54_12820"
     CDS             426..1700
                     /gene="urtA"
                     /locus_tag="VFA54_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HZS51741.1"
                     /translation="MSRRKEKGLANPGRRAFLKKTAGAALAAAASGLTGLSCGGRSSQ
                     PIKVGIMHSLSGTMAISETSLRDVELMAIEEINAAGGVLGRQVQAIVEDPASDWPTFA
                     EKSRKLLLSDQVCSVFGCWTSASRKAVLPVFEKNNGLLWYPVQYEGNECSHNVFYTGA
                     APNQQIGPAVTWLLKNRGKKFYLLGSDYVFPRTANGIIKGQLAQEGGQLVGEEYTPLG
                     HMEYSTVVNKIRAAKPDVVFSTINGDSNVAFYKQLRNAGITADDIPVMAVSVAEDEVR
                     GIGGSVAAGHYASWNYYQSVDTPENKAFVAKFKAKYGDNRVTDDPIEAAYFQVHLWAQ
                     AVQKANSIEVDAIRAAALGQEFKAPEGLVRIDPKNQHTWKTVRIGKIREDGQFDIVWS
                     TDGPIRPEPWDKIQFPKRDCDWTRGGTVENAS"
     gene            1926..2837
                     /gene="urtB"
                     /locus_tag="VFA54_12825"
     CDS             1926..2837
                     /gene="urtB"
                     /locus_tag="VFA54_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HZS51742.1"
                     /translation="MTPNTFLLGQTFNGLSYCSILLLISLGLAITFGLMGVINMAHGE
                     LMMLGSYTAYVLQNIFLRHYSEHFSYYFAIALPLAFVVAGAVGVLLERSLIRFLYGRP
                     LETLLATWGVSLILQQLMRQIFGANNVDVRSPAWLTGRWEPFPGLELPHIRLFIIAFT
                     AVCVLLMYAVLFRTTLGLKIRAVTQNRDMSACLGISTGKVDAYTFAIGSGLAGMAGCI
                     ISLMGSVGPNTGQNYIVDAFMVVVLGGVGKLAGAIAGSFAIGEVNVLFESFSTATMGK
                     VLVFTVVILFLQWKPSGVFPSKVRSLD"
     gene            2834..3961
                     /gene="urtC"
                     /locus_tag="VFA54_12830"
     CDS             2834..3961
                     /gene="urtC"
                     /locus_tag="VFA54_12830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HZS51743.1"
                     /translation="MKTRVANEWLVFAALLAVLLFAAPNALSPFRLNLLGKFLALAIL
                     ALGIDLIWGYTGILSLGHGVFFGLGGYCMAMYLKLAVGAAGTYMVALPDFMVWNGLKA
                     LPWFWKPMYHFWIAIPLAVLLPALCGAILGFLTFRSRVQGVYFSIITQAMALVASLLL
                     IGQQPYTGGTNGITDFNDILGFPIAAKKTQTGLYIITVLCLAACYVLCRWIVRSKFGR
                     VLAAIRDAENRVRFLAYDTVMFKVFVFSISAALAGLAGALFVPQVGIISPSNIGVVPS
                     IEMAIWVAVGGRGTLSGAVLGAVLVNAAKSAFSESFPDFWLYFLGLLFIGVVLFFPEG
                     CVGIFLRARERFRRPARKSPGAELKSEMEKPVPQRAGGVSQ"
     gene            3961..4728
                     /gene="urtD"
                     /locus_tag="VFA54_12835"
     CDS             3961..4728
                     /gene="urtD"
                     /locus_tag="VFA54_12835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HZS51744.1"
                     /translation="MAESIIYLEGVTVSFEGFKALNNLNFIVDRGELRVVIGPNGAGK
                     TTLLDVICGKVRPAEGRVIFGDMTDICSLREHEIARLGIGRKFQTPSIFKNLTVFENL
                     DLAARDKSKGVFSTLFASPSPVKREAIFSTLETIGLASKSAQIAGSLSHGEKQWLEIG
                     MVIMQDPKLMLIDEPVAGMTGKETERTGELLQSIAGERSLLVIEHDMVFVRQIAKKVT
                     VLHEGSVLCEGSMETVQSDARVKEIYLGREAREHAGN"
     gene            4715..5425
                     /gene="urtE"
                     /locus_tag="VFA54_12840"
     CDS             4715..5425
                     /gene="urtE"
                     /locus_tag="VFA54_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HZS51745.1"
                     /translation="MPETDSLRIESLNVYYGESHILRDVALRVPQCKTACLMGRNGAG
                     KTTLLKSLIGLLPARGGRIIFEGQDVTSWPPDHRARSGIGYVPQGREILPQLTVQENL
                     TLGLMAGPRQNGGLLDEVFHLFPVLKTMLKRRGGDLSGGQQQQLALARALLAQPKVLL
                     LDEPTEGIQPSIIDEIEDVLVRIKEQKAVSILLVEQYLEFALRLADYFYIMEKGSIVA
                     QGPIGELSAEVARRHLEI"
     gene            complement(5454..6182)
                     /locus_tag="VFA54_12845"
     CDS             complement(5454..6182)
                     /locus_tag="VFA54_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS51746.1"
                     /translation="MKFPIASFLIYFALAAAAAGISQAADVIQTNAGDLTITPIHHAS
                     LMLQFEGKVIHIDPWSQGNYSSLPRADYIFITDIHGDHLDKGMIDKLKKESTAVVGPQ
                     AVADQVHGITVLKNGETKKFDGFEVEAVPMYNLKRGPQPGALYHTKGRGNGYVFTMGG
                     KRIYVSGDTEAIPEMRELKNIDVAFICMNLPYTMTPEEAAEAVKAFRPKIVYPYHYQK
                     SDVNAFANALKGEKGIEVRLRDWY"
     gene            complement(6284..7873)
                     /locus_tag="VFA54_12850"
     CDS             complement(6284..7873)
                     /locus_tag="VFA54_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HZS51747.1"
                     /translation="MPAAAVSTPTEISGKFLTIDDIAGIARHYQPVRLPSSEDFRRRI
                     ERGPEAVRAMLERGELIYGVNTGFGGNVKFIIPDEELQHHQENLLRFLSCGVGDPLPE
                     DAVRAAILLRANALARGYSGVRWVVLERLLDLLNHGITPIVPRYGSVGASGDLCPSAY
                     VARTLLGQGTVRYRGAVVPASTALAGEGLEAIRLQAKEGLALVNGTTTMTGIAAMVVW
                     DADYLLRIGLAAVALAVEALESSPDYFHPLIHMVKHHPGQLRAAEMLNSLLMDSKLAV
                     PLNEIRRRAAAENVHSHEVVVAHEAIQSPYSLRCVPQGLGPMLESLEQARLVIEREAN
                     SVNDNPLIDPEQGRVFHTGNFYGGHIARAMDGIKLDMANLANWMHSLMALLMDERFSA
                     GLPNSLSPHPGVYQGFKGMQIVHTSLVTALRQMSAASLIHTLPTEQFNQDVVSLGAHA
                     ALTAMDMVRVLRDSMSIVLLALAQAIDLRKGAEILGAGTGPVYRSIRELGAFVDADRP
                     LDDDIRRLSEALEAHKVPVLQ"
     gene            8049..9260
                     /locus_tag="VFA54_12855"
     CDS             8049..9260
                     /locus_tag="VFA54_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZS51748.1"
                     /translation="MRSKFRRFCGMAALAVVSAVASQAAAPEHAILWVVDGFSSQAPD
                     RVNLKNLKALMKEGVYYRNNYTVQTADPSGQPGMWSEYHTSSIPNPVLLAGTAMLLPG
                     KQHYVQESFFPLKITAHAVNEISYRALNVGFHFTAQAGGQLMIRAGYKTGDDRTLYWA
                     TQFLKVAHPAFMLIHMQDTGFAGGASRTAPPGSPYKDNIWGEGSPYMKTVAQQDVYLG
                     QFIDELKKEGIWDKTIIFITGDHGQTTLGWHPPEASDAWTMPLVMAGPGIKKGQTFDY
                     AESIDVVPTLCYLMGVRPPINSDGRILAEALVDPPKDVPPRQQKIKELDFLLLDVQKK
                     LEALEAKAPRGRRDIRDMSPLAQAERDFYRIERILEWHQFGTYDRLIAHHKALLQRLD
                     AMAANGGAAGN"
     gene            complement(9675..10262)
                     /locus_tag="VFA54_12860"
     CDS             complement(9675..10262)
                     /locus_tag="VFA54_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZS51749.1"
                     /translation="MSESKAAAEKAGYFLADIREIRRRARQHIDQGAVTEAYKGDRET
                     VIRVLNEALATELVCVLRYKRHHFMAKGIHSQAVAQEFLEHAAEEQVHADQIAERIVQ
                     LDGEPNFNPEGLATRSHSEYVEGETLVDMIREDLVAERVAIESYAEIVRYLGNNDPTS
                     RRLMEEILAKEEEHAEDMKTLLENIEKEAEQRRRG"
     gene            10705..14670
                     /locus_tag="VFA54_12865"
     CDS             10705..14670
                     /locus_tag="VFA54_12865"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HZS51750.1"
                     /translation="MKLPVWISALVVLAAFALRAQPARTPPPGDSEKPAEIAIFYDGP
                     TQPLAEGYLDALQIENLLGHFGLRGRHIPIAAYQPGELAGYRAAFFIGTVSGTRFPQG
                     FLGEVLASHQPFCWIGRHIGSLANSPRGRRLGFTYIDYRDDLEFRQVVYKNTRLPKGD
                     PDLNIVSVTDPRSVQVLAVAVNDENDRHPYALRQGRFWYFADSPFSYVEEGSRYLVFC
                     DLLHDILEIPHGASARALVRIEDVSTEIDPGDLRAVADTLAARRIPFQISIIPIFRNP
                     SKGFEVYLSDRQNLVDAIHYMIARGGAPVMHGTTHQYRGPSGDDYEFWDEAGGRPIQR
                     DSAGLVLERLGLGWKECLANGIFPIAFETPHYGASETDYLAMRQVFSLFNERTMPTPD
                     VASIQYFPYPVIDRFGRYVVPENLGYLPAENPDPRILIERARNMRVVRDAIASFYFHP
                     FLDPALLAETARGITEAGFRFVSLREFGGAVDLGGHYALRTSSDRVRLTPQKQYWRSR
                     GFDGAGKLVSETVSASPSSGAVEVDVRPPAGGWAVVDCIAEAPRRTSTAGWRARLAQW
                     ESRLWPARRFLGGNGDLAPSPRAWVLWLSAASPAEDNNQRSYRTLLETFGYQTAILPL
                     DRFRAGPAGRGTILVVPEGAGEHLEPFQQDAVLSYIRAGGLAVLEGRQKWLEPLGFRW
                     SGHSITVSTVTDVLYPEMDLRWRPEDRVERFTPPEGAEQLMVEPQSKQLLALAGGYGS
                     GRYVYLAVPLDPHTPGATSHYPYFPEYLAEAFRLPPSPRSPGIEAYFDPSYREGANLE
                     RLAASWRQEGIRTIYAAAWHFYRDYAFDYERLIAACHRNGIAVYAWLVLPATTPLMWD
                     QHPEWRERTASGGAGRVSWRYSMNLQNPACFRAAMEWMNGLLAGRAWDGVNISELNFD
                     ADFEDYLRPDRFVPMNADVRADFRSQAGFDPVLLFAPASPYYYKRNPQALERFLKYRE
                     DVVATWHERVLSDLAPLARARGWEVIITMLDSLHSRYVQPALGVDSRRIIALMSRFDF
                     TLQVEDPSEHWIAPPDRYRRFAETYRRLVPDPRRLMFDINVVPDRGVEGTSLPSSTAA
                     GVELARTAAAAASASGRAAIYSEYTVPAQDWRLIGTALAGPAAVSAANAGWRLHSPVP
                     LLLGSPAGAEYYLDGRLWPARLSDGLLVPAGRRSLSAVRPWYAALDRGSPPARLVDLN
                     GELLDARARRTGIEIQYRSPGRAVLVLNQKPREMFVDGAPAQAQWERASGGDWVVAAP
                     RGEHRIVVETFTRAGVLVNFWGWMSASAIAGFGALTTALMAVIYLHIRMRRLARRGGL
                     S"
     gene            14667..15470
                     /locus_tag="VFA54_12870"
     CDS             14667..15470
                     /locus_tag="VFA54_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51751.1"
                     /translation="MTGAALAACLATLAAAIPGAAQTPSAPRRVSAPISFYDDADPTD
                     PGVLSASASFSYTRVDAGHDLDGPSAYLSLGLHKRIEVSADLGRAESLFEETRISGIA
                     DSYLAAKFLVFPEGRRRPALALKPMLEILGPASIHQDVLAPGRVNFVPSFILQKSFDD
                     FRVYYMGGYVTRGILFHSLAGELNLWSRVTPTVILSAARVTREAALISDLGLNRSRAD
                     ATVSLTIMLTPRWSVFAGGGRSFGRSDLNSSCYAFSGGAAFHFRLWGEK"
     gene            15467..17563
                     /locus_tag="VFA54_12875"
     CDS             15467..17563
                     /locus_tag="VFA54_12875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HZS51752.1"
                     /translation="MSRNFFLAAALSVMPMLAQGVAYIVDNRNGQVDVFDLGSWRVIS
                     SIRTGNQAGEIYILPNNRFAFVSNQGDSNVTLLDLQAGAFVTSLPTGQGPGSLAGTPD
                     GRLLYVANEGSNDVTVIDVARRAVVATIPVGTTPVQVNLSPDGLFVYVVNQDDGTITV
                     IDAIRNQVKQTLPVGQQPNQIAIAANLNTAYILNTGSNSVSLLDLSTNTITGSVPVGA
                     GPVTAAFSSDGQRLFVVNRDSNSVSIIDTRQNRVIATIGVGARPVAIAVTFDGKFAYV
                     SNQGDGTVSLLDLTANAQADVISVGGSPFSVQFDPNENFLYVTNLTSGSISVIDTSTD
                     KPTSVPVGGAPVQFAFLNAPTLLEISPNPAPVCSLITLKGEGFVPASAVWYNRSAFAG
                     AASACPAAPAPAGFVDSETWQAIVPPLASSSAGISVVNPDGNASEQLVLQIAPSPSPV
                     IISPGGVVEGAGFQKAPYPISGGSIVSVFGSFPGASTDQARNFPLPTTLANVRVDFNG
                     VPAALLYASPAQINAVAPAGLLALAAGLGNGQVTPVQVSVTVLGPPGSPPYVSAPAPV
                     SVAAAAPGIFSDPASGIGAFLHGADLSPVTTANPARAGETIVLYCTGLGNTSPPPMDG
                     QPAPADILSPAASLPVVTVGGAQANVRFAGLAPLYAGLYQINFDVPSGTPGGAAMVKV
                     TSASWTSNGVLLPTRP"
     gene            17567..18829
                     /locus_tag="VFA54_12880"
     CDS             17567..18829
                     /locus_tag="VFA54_12880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS51753.1"
                     /translation="MYSSIVLDILFIVAVTLIWFMLAYQSLLFFLGHRYYRLTRRSTR
                     PPRAALPDSALPCISLLLPCRNEERVIARTLRALLALDYPGDKLEILVINDASEDRTA
                     EIARTFASDARVRLLDVPMTEAARGKAAALNFALRHARYPVLAIYDADNTPEPGALRP
                     LVEQLLADPSLGAAIGVYRAVNRRRALLTRFLNIEGIGFQWIIQAGRWMLMRLTTLPG
                     TNYVIRRELVESLGGWDEQALTEDAELTIRVYQAGYLIQFVPASVTWEQEPETIRVWF
                     RQRHRWVRGYNYLFRKYAGKLLQARPRRVGLELLYSLSLYYVFFLAIVISDALFVLSS
                     ARVIHIGVPGPFSILWLFAYVTFVLQLVIALSCEPGEDSLLNILLTMVMYFSYCQLWI
                     PVVARAFFDDFIVRREIKWAKTERYEVR"
     gene            18896..>20415
                     /locus_tag="VFA54_12885"
     CDS             18896..>20415
                     /locus_tag="VFA54_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51754.1"
                     /translation="MGRCGKPAASKAAALLLLAGSLTRLQPSPHGCPSGAPIATFQLS
                     VENTSSEPLLPVRSVSQIRSGQHIFYEPLDLTASLKKSGKIALILAPAGLPASGHLTV
                     LDPKPAAQPAQWAVPYQTEVLGLVVGPQGLDVAKVSSLLDQDHELMSQLADYAAQSTQ
                     VESLIEKLSSAEQETDKGAALSAALGGLAGSSPSKLDSNASAEAQAMALLRALNPAVG
                     AYNPLAPQPAARMQQSAGLAASLAGLFLGNPVGLAVDGAAMLNNLRTLLFPGTDFRSA
                     LARPAFSDGMTLCAAQGDVKSRARLAYLWALRLPNDVAPAISVGDSVHLPIGVKWAVP
                     VKWEGASKFPPLERVREWTLVPQDDSAGIPVTVKPLPQFRSLEVDLSRFSGPPGVYRL
                     TGKWDWDRLDVAGSFRLFSLSTLEKARLTEASQDRLVSGSGQAAIELEGGDFQFVQSV
                     GLRDAARPGAKPAELTFHTLPLRGAAAPANLEVLVNLREWKAGEYQLLLTQPDGSI"
BASE COUNT         3590 a         6506 c         6106 g         4213 t
ORIGIN      
        1 aacttcgagt tcgtcgtccc aatcctcgcc tccgtgcggt ggtccggcaa gatcggccac
       61 ggccaggggc tgggcgacga gttcttccgt ttcggccaag cgttcaacaa ccgcggcctg
      121 ccgatccgga ccacggccgg ctggacggaa ctcggatatt acggctcgaa gtggtccgtg
      181 gccggcggct ggggcgacga cgatcccaac gacaccgatc tccgcagcat ctcaaacaac
      241 aacggcaatt acacccacaa ccagcgcatc ttcgtgaatg cgctgtacga tctgttcgct
      301 cacgtcaagg tcgggggcga atacaactac ctgcgcacca actggacgaa tggagatctg
      361 ttcaaggccc atcaggtcat gggcgttgtc ttctattcgt tctgaatttg aatcaggaga
      421 aacgtatgtc aagacggaaa gagaaagggt tggcaaatcc agggcgccgg gcgttcttga
      481 aaaagacggc aggagcggcg ttggcggctg ccgcgtcggg cctcacgggg ctttcctgcg
      541 gcgggcgttc ttcgcagccg atcaaagtgg gcatcatgca ctccctcagc ggcacgatgg
      601 ccatcagcga aacctcgctc cgcgacgtcg aattgatggc cattgaagag atcaacgctg
      661 cgggcggcgt attgggccgg caggtccagg ccatcgtgga agatcccgct tctgactggc
      721 ccaccttcgc ggaaaaaagc cgcaagcttc tgttaagcga tcaggtctgc tcggttttcg
      781 gctgctggac ctcggccagc cgcaaggccg tgcttccggt gtttgaaaaa aacaacggct
      841 tattgtggta tccggtgcag tacgaaggca atgaatgctc ccataacgtt ttctacaccg
      901 gcgccgcgcc taatcagcag atcgggccgg cggtgacgtg gctcctgaag aaccgaggca
      961 agaagttcta cctgctggga tccgattacg tcttcccgcg caccgctaac ggcatcatca
     1021 agggccagct cgcacaggaa ggagggcagc tggtcggaga agaatacacg ccgctcggcc
     1081 acatggagta cagcaccgtc gtaaacaaga tccgggccgc gaagccggac gtcgtcttca
     1141 gcaccatcaa cggcgacagc aacgtcgctt tttacaagca gcttcgcaac gcgggcatca
     1201 ccgcggacga catcccggtc atggccgtga gcgtcgccga agatgaggtg cgcggcatcg
     1261 gcggctccgt ggctgcgggc cattatgctt cctggaatta ttaccagagc gtcgataccc
     1321 cggaaaacaa ggccttcgtc gcgaaattca aggccaagta cggcgacaat cgcgtgaccg
     1381 atgacccgat cgaggcggcc tacttccagg ttcatctctg ggcccaggcc gtccagaaag
     1441 ccaactccat cgaagtggac gccatccgcg ccgcggctct cggccaggag ttcaaggcgc
     1501 ctgagggctt ggtccgcatc gacccgaaga accagcacac ctggaagacg gtgcgcatcg
     1561 ggaaaattcg ggaagacggc cagtttgaca tcgtctggag caccgacggc ccgatccggc
     1621 ccgagccttg ggataaaatc cagttcccca agcgggattg cgattggacc cggggaggaa
     1681 cggttgaaaa cgcgtcctag cgtcgcgggt atcggaggcc cgccggcgaa gtacggccgc
     1741 cttcctctca agcccgcgct ttcgccggtg tatggcgcgg cggtggggag ggagcggtgg
     1801 cggcgggatg gaattcgttg aaattctgag acacggcgcc ggccgcttac cgtaggtcgc
     1861 cgcgagcgta gagagtataa ttggtgtttc tttgcgctcg cgtcgtctga cagcgatagg
     1921 ttgacatgac gcccaacacg tttctgctgg gccagacgtt caacggcctc agttattgtt
     1981 ccatccttct gctcatttca ctgggccttg ccatcacctt cggcttaatg ggggtgatca
     2041 acatggctca tggcgagctg atgatgctgg gctcgtatac cgcctatgtg ctgcaaaata
     2101 ttttcctccg gcattatagc gagcacttca gctactactt cgccatcgct ctgccgcttg
     2161 cgtttgtagt ggccggcgcg gtcggcgttc tgctcgagcg ctcgctaatc cgtttccttt
     2221 acggccgccc gctcgaaaca ctgctggcca cctggggagt cagcctcatt ctccagcagc
     2281 tcatgcggca gattttcggc gccaacaacg tggacgtcag gagccctgcg tggctgacgg
     2341 gtcgctggga gcctttccct ggactggagt tgccccacat ccgcctcttt atcatcgcct
     2401 ttactgccgt ttgtgtgctg ctcatgtacg ccgtgctgtt ccggacgact ctcgggttga
     2461 agatccgcgc cgtaacccaa aatcgagaca tgagcgcatg cctgggcatt tcaaccggca
     2521 aagtggatgc ttacacgttt gcgatcggct ccggcctggc gggcatggcg ggctgcatta
     2581 ttagcttgat gggttcggtg gggccgaata ccggacagaa ttacatcgtg gacgccttca
     2641 tggtcgtggt gctcggcgga gtcggcaagc tggcgggagc aatcgccggc agcttcgcga
     2701 tcggtgaagt caatgtgctg ttcgagtctt tcagcacggc cacgatgggc aaggttttgg
     2761 tctttactgt cgtgatcttg ttcttgcagt ggaagccgtc tggcgtattt ccatcgaagg
     2821 ttcggagtct cgattgaaaa cgcgagttgc caacgagtgg ctcgtctttg cggcccttct
     2881 ggcggtcctt ctttttgccg cgccgaacgc tctttccccc ttccgtctca atttgctggg
     2941 caaatttctt gctctggcta ttcttgcgct gggtatcgat ctgatctggg gttacaccgg
     3001 aatcctgagc ctggggcacg gggttttctt cgggctgggc ggctactgca tggcgatgta
     3061 tctcaagctt gcggtcggcg ccgccgggac ctacatggtg gctctgccgg atttcatggt
     3121 ctggaacggc ctcaaagcgc tgccttggtt ttggaaaccg atgtatcatt tttggatcgc
     3181 cattccgctc gccgttctgt tgccggcgct gtgcggggcc attctcggat tcctaacctt
     3241 tcgcagccgg gtccagggcg tctatttttc gatcattacg caggccatgg ccctcgtcgc
     3301 cagcctcctg ctgatcggcc agcagccgta tacgggcggc accaacggga tcacggattt
     3361 caacgacatt ctcggcttcc ctatcgccgc caagaagacg cagaccggtt tgtatatcat
     3421 aaccgtgctt tgcctggccg cgtgctacgt tctttgccgc tggatcgttc gctccaaatt
     3481 cggccgagtt ctcgccgcca tccgcgacgc ggaaaaccgg gtgcgctttc tggcctacga
     3541 cacggtcatg ttcaaggtgt ttgtcttcag catctcggcc gccttggccg ggctggccgg
     3601 cgccctgttc gttccccagg tgggcatcat ttcgccgtca aacatcggcg tagtgccctc
     3661 gattgaaatg gcgatctggg tggcggtcgg cggccgggga acgctctccg gcgctgttct
     3721 cggcgctgtg ctggtcaacg cggccaagag cgcattcagc gagtcgttcc ctgatttctg
     3781 gctctacttt ctgggtttgc tttttatagg cgtggttttg tttttccccg aaggctgcgt
     3841 cggcattttc ctccgcgcgc gggagcgatt ccggcgcccg gccagaaaat cgcccggcgc
     3901 cgagctgaaa tccgaaatgg aaaagccagt gccgcagcgt gccggaggcg tgagccagta
     3961 atggcggagt ccatcattta tctggaaggc gtcacggtca gctttgaggg ctttaaggct
     4021 ctcaacaatc tgaatttcat tgtggatcgc ggcgagctcc gcgtcgtcat cggcccgaac
     4081 ggcgccggga agaccacgct gctggacgta atttgcggca aggtccggcc cgccgagggg
     4141 cgagtcatct tcggcgacat gactgacatt tgctctttgc gggagcacga aatcgcccgg
     4201 ctgggcattg ggcgaaagtt ccagacgccg tcgattttca agaatctgac cgttttcgaa
     4261 aacctggatc tggccgcacg cgacaaaagc aaaggagtgt tttccacgct ctttgcttcg
     4321 ccctcgccgg tcaagagaga agcgattttt tcgaccctgg agaccatcgg ccttgccagc
     4381 aagtccgcgc agatcgcggg atcgctttcc cacggagaaa aacagtggct ggaaatcggc
     4441 atggtcatca tgcaggaccc caagctgatg ctcattgatg agcctgtcgc cggcatgacc
     4501 ggcaaagaaa ccgaaaggac cggcgaactt ctccaatcga tcgccggcga acgctccctt
     4561 ttggttatcg agcacgacat ggttttcgtc cggcaaatcg ccaaaaaagt gacggtgctt
     4621 cacgaaggct ccgtgctctg cgagggatcg atggagacgg tgcagagcga cgcccgcgtg
     4681 aaagagatct atttgggccg cgaggcgcgt gagcatgccg gaaactgaca gccttcggat
     4741 cgaaagctta aacgtctatt acggggaaag ccacattttg cgggacgtgg cgctgcgcgt
     4801 tccccagtgc aaaacagcct gcctgatggg ccgcaacggc gccggcaaga cgactttact
     4861 caaaagcctg atcggccttc tgcccgctcg cggcggacga atcatttttg agggccagga
     4921 tgtcacctcc tggccccccg accaccgcgc ccgttccggc atcggctatg tgccccaggg
     4981 acgggaaatc ctgccgcagt taactgtgca ggaaaacctg acgctcggcc tgatggccgg
     5041 cccccggcag aacggaggcc tgctggacga ggttttccat ctgtttcccg tgctgaagac
     5101 catgctcaaa cggcgcggcg gggatttgag cggcggtcag cagcagcagc ttgctctggc
     5161 ccgcgccctg ctcgcccagc cgaaggtgct cctgctcgac gaaccgaccg agggaattca
     5221 gccgtcgatc atcgatgaga tcgaggacgt cctggtccga atcaaggaac agaaggccgt
     5281 ctcgatcctg ctggtggaac aatatctgga gttcgccctg cgcctggcgg attacttcta
     5341 catcatggag aaaggctcca ttgtggcgca aggcccgatc ggcgagctta gcgccgaagt
     5401 cgctcggcgg cacctggaga tttgattttt tcagggattc ctgcccgccg ccgctaatac
     5461 cagtcccgca accggacttc aatgcctttc tcgcctttga gcgcattggc gaaagcgttt
     5521 acatcgctct tctggtagtg gtatggatag acgatcttcg gccggaacgc cttcaccgct
     5581 tccgcggctt cctcgggcgt catcgtgtag ggaaggttca tgcagatgaa cgccacgtcg
     5641 atgtttttca gctctctcat ttccgggatg gcttcggtgt cacccgagac gtagatgcgc
     5701 ttgccgccca tcgtgaagac atagccgttg ccgcgcccct tcgtgtgata gagcgcgccg
     5761 ggctggggtc ctcgcttgag gttgtacatc ggcacggctt ccacttcgaa gccgtcaaat
     5821 ttcttcgttt ctccgttttt cagaacggta atcccgtgga cctggtcggc cacggcttgc
     5881 ggcccgacaa cggccgtcga ttcctttttc agcttgtcga tcatgccctt gtcgaggtga
     5941 tcgccatgga tgtcggtgat gaaaatatag tcggcccggg gcaggctcga atagttgccc
     6001 tggctccacg ggtcgatgtg aatcaccttg ccttcaaact ggagcatcag gctcgcgtga
     6061 tggatcgggg tgatcgtcag atcaccagcg tttgtctgga tgacgtcggc cgcctgcgag
     6121 atgcccgcgg cggcagcggc cagcgcgaaa taaatcagaa aagaagctat gggaaacttc
     6181 atgccatcga tttaactaca cttggcgcct gactcgcaat ccgcgcacct tctccaaggc
     6241 ggcaaatcgt ggagtaggaa agctggcggg aaagccaacg gcgctattgc agcacgggca
     6301 ctttgtgggc ttccagcgct tccgacaggc gtcgaatgtc atcgtcgagc ggacggtcgg
     6361 cgtcaacaaa agcgccgagt tctcggatgc tccggtaaac cggccccgtg ccggcgccca
     6421 gaatctccgc gcccttccgt aagtcaatgg cctgggccag cgccagcagc acaatcgaca
     6481 tcgaatcgcg cagcacccga accatgtcca tcgccgtgag cgcggcatgc gctcccaggc
     6541 tgaccacgtc ctgattgaac tgctcggtgg gcagcgtatg gatcaagctc gccgccgaca
     6601 tctggcgcag cgccgtcacc aggctggtgt gcacgatctg catgcctttg aatccctggt
     6661 atacgccggg atgcgggctc aacgagttag gcagcccggc cgaaaaccgc tcgtccatca
     6721 gcagggccat gagtgagtgc atccagttcg ccaggttggc catgtcgagc ttgatcccgt
     6781 ccatcgcccg cgcgatgtga cctccataga agttgccggt atggaaaacg cgtccctgct
     6841 cggggtcgat cagcggattg tcgttcaccg agttggcctc gcgctcgatc accagccggg
     6901 cctgttccag gctctccagc atcggcccga gcccctgcgg aacgcagcgg agcgaatagg
     6961 gactctgaat cgcctcatgc gccactacta cttcatggga atgcacattc tccgccgctg
     7021 cgcgccggcg aatttcgttc agcgggacgg ccagcttgct gtccatcaac agagagttca
     7081 gcatttccgc cgcccggagc tggcccggat ggtgcttcac catgtgaatc aagggatgga
     7141 aataatcggg cgacgattcc agcgcctcta ccgccagcgc cacggctgcc aggccgatcc
     7201 ggagcaaata gtcggcatcc cagaccacca tcgccgcgat gcccgtcatg gtggtcgttc
     7261 cgttaaccag cgccagcccc tccttggcct ggaggcggat cgcctcgagt ccctcgcccg
     7321 ccagcgctgt tgatgcggga acaacggcgc cccggtatct caccgtccct tgacccagca
     7381 gagtccgcgc tacgtaggcc gaagggcata aatcaccgct ggcgcccacc gacccgtaac
     7441 ggggaacaat cggcgtgatc ccgtgattca gcaaatcgag caaccgctcc agcactaccc
     7501 accggacgcc cgagtaaccg cgcgccagag cgttcgcccg gagcaggatg gcggctcgca
     7561 ccgcatcctc gggcaatgga tctccgacgc cgcaggacag aaagcgcagc aggttctcct
     7621 gatgatgctg caactcttca tccgggataa tgaatttcac attgccgccg aacccggtat
     7681 tgacgccata gatcaactcc ccgcgctcca gcatggcgcg aacggcttcc ggcccgcgct
     7741 cgatccggcg ccggaagtcc tcgctggacg gcagccgcac cggctggtaa tgccgggcaa
     7801 tgccggcaat gtcgtcaatg gtgaggaact ttccggagat ttcggtaggc gtcgagacgg
     7861 cggcggcggg catggcgtcc tccctgtggg aaatataaca ccacaacacc actattaggt
     7921 ggtgctatat atagtatccg ccttccggcc gcctcgggtc aaccggatgg cgttcgggtg
     7981 caatgcctgg atgtttggcg tatgatgtaa ggcgctttgc gcaaaatccc caggagaggt
     8041 atccggaaat gagatccaaa tttaggcgct tttgcggcat ggccgctctg gcggttgttt
     8101 cggccgtggc atctcaggcg gcggcgcccg agcacgccat attatgggtg gtggacggct
     8161 ttagttcaca ggcgcccgat cgcgttaatt tgaaaaacct aaaggcgttg atgaaggagg
     8221 gcgtatatta ccgcaacaac tacacggtcc agacagccga tccctccggg cagccgggca
     8281 tgtggtccga gtaccacact tcttcgattc ccaacccggt cttgctggcc ggcacggcga
     8341 tgctgctgcc gggcaagcag cactacgtgc aggagtcgtt ttttccctta aagatcacgg
     8401 cccacgccgt caatgagatt agctaccggg cgctcaacgt tggtttccat ttcacggccc
     8461 aggcgggcgg ccagctcatg attcgggcgg gctacaaaac cggagatgac cgcacgcttt
     8521 actgggcgac ccaattcctc aaagtggcgc atcctgcctt catgctgatc cacatgcagg
     8581 atacgggctt cgccggcggc gccagccgaa ccgcccctcc cgggtctcct tacaaggaca
     8641 atatttgggg tgaagggtcg ccttatatga agacggtggc tcaacaggat gtttatttgg
     8701 gccagttcat cgacgaattg aagaaagagg gaatctggga taagacaatc atcttcatca
     8761 ctggcgatca tggccagacg acgctcggct ggcatccgcc ggaggcgagc gacgcctgga
     8821 cgatgccgct ggtaatggcc ggtccgggaa taaaaaaagg ccagaccttc gactatgccg
     8881 agtccatcga tgtcgtcccc actctctgtt atttgatggg cgtccggccg ccgatcaatt
     8941 ccgatgggag aatccttgcc gaagcgctgg ttgatccgcc gaaagacgtg ccgccccgcc
     9001 agcagaaaat caaggagctc gatttcctgc tgctggatgt gcagaagaag ctcgaagccc
     9061 tggaagccaa ggcgcctagg ggccgcagag acattcgcga tatgtcgccg ctggcgcagg
     9121 ccgaaaggga tttttaccgg atcgagcgca ttctggaatg gcaccaattc ggaacttacg
     9181 accgcctcat cgcccaccac aaagcgctct tgcagcgcct ggacgcgatg gccgcgaacg
     9241 gcggcgcggc cggcaactaa acgaaccgga aagctccggt tgcatcccgt ttacgcgtgc
     9301 ggccgccgcg ccggccagac ggcggcggcc gccttacatt cagggaacaa gaaaatcaat
     9361 ctttggaagt cccccgaagg ttcactattc gccagagcag acggacttct ggaatccgcg
     9421 ctatcgatcc aaacgccgca gaggcgagaa tagaaaccaa atcaagcggt cagaaggcag
     9481 tctgcggttc cacgagccga ttcagccctt ggatcccgga tgatacgcgg ctccggagtc
     9541 gactcggtag ccggtccccc agcctgttgc ccgcgaagag tgtaatcggc gttgcctacg
     9601 cgccctcggc tgcttcgcat cttcggcgtc cttcaggaac tcgtccgcct tgcgcggcag
     9661 cggtcttgga agcctcagcc gcggcggcgc tgctcggctt ccttttcgat attttcgagc
     9721 agcgttttca tgtcttcggc gtgttcctct tctttggcga gaatttcttc catcaggcgc
     9781 cggctggtcg ggtcgttatt ccccaggtat cggacgatct ccgcgtagga ttcgatggcc
     9841 actcgctcgg ccaccagatc ttcccggatc atatccacca aagtttctcc ttccacgtat
     9901 tccgagtggc tgcgggtcgc caaaccttcc ggattgaaat ttggctcgcc gtcgagttgg
     9961 acgattcttt cggcgatctg gtcggcatgg acctgttcct ccgccgcgtg ctccaaaaat
    10021 tcctgcgcca cggcttgcga atgaatgccc ttcgccatga agtggtgccg cttgtaccgg
    10081 agcacgcaca ccagctccgt cgccagagct tcgttcagca cgcggatcac ggtctcccga
    10141 tcgcctttgt aagcctctgt gacggcccct tgatcgatat gctggcgggc gcgccggcga
    10201 atttcgcgga tgtccgccag aaaataaccg gccttttcgg ccgcagcttt gctctcgctc
    10261 attttttcag cctgcctttc gtcggcggct tgcgtgcgcc gatcttggta aatctttacc
    10321 gttccccacc tccgattggc ttgagaacgg tctcaccagt tagcaccaac ggggccacac
    10381 cctcgactag cttgcatgat ttgtaacgaa tgcccccgcg cgcccggcaa gcgaggcacg
    10441 cggccaactc gcccccggcg ggcagggtgt ttattcgaca ggggaacccg tctttccttc
    10501 cacctgcgtt ccacctgcga gccgctttct ttgcggccaa gcaacgcggc ggccaaacag
    10561 gccaatccgc cgctgacaac gaatcaagga cattcaaggc aaaagggttc gccgtccagc
    10621 ggtccagagc cggcatgcgt ccctgtgagg gaacggacgt tctgttataa tatccccagt
    10681 ctgcgtccct ccttactgtg agtaatgaaa ctaccggttt ggataagcgc tttggtagtg
    10741 ttggcggctt tcgcccttcg ggcgcaaccc gcccgcaccc cgccccccgg cgactctgag
    10801 aagcccgccg agatagccat cttttacgac ggccccactc agccccttgc ggaaggctac
    10861 ctcgatgctt tgcagatcga aaacctgctt ggccacttcg gcctgcgcgg ccggcacata
    10921 cccatcgccg cttaccagcc cggcgaactg gccggctatc gcgcggcttt ttttatcgga
    10981 actgtctcgg gcacgcgttt cccccaaggc tttttgggcg aggtgctggc ctcgcaccag
    11041 ccgttttgct ggatcggccg ccacatcggg agcctcgcga attcgcctcg cgggcgccgc
    11101 ctcggattca cctacatcga ctaccgcgac gacctcgagt ttcgccaggt ggtttacaag
    11161 aacacgcgtt tgcccaaggg agatcccgat ctcaacatcg tctccgtcac cgatccgcgt
    11221 tcggttcagg tgctcgctgt cgccgttaac gacgaaaacg accggcatcc gtacgcgctg
    11281 cgccaaggcc ggttctggta tttcgccgat agcccttttt cttacgtgga ggaaggcagc
    11341 cgctatctgg ttttctgcga tctcctccat gacattctgg agattcctca cggcgccagc
    11401 gcccgggcac tggtgcgtat cgaggacgta agcaccgaaa tcgatcccgg tgatctgcgg
    11461 gccgtggccg acactctcgc cgcccgccgc attccgttcc agatcagcat catcccgatc
    11521 tttcgcaatc catcgaaagg atttgaagtc tatctttccg accggcagaa tctggtcgat
    11581 gccatccatt acatgatcgc tcgcggaggc gcgccggtga tgcacggcac gacgcaccaa
    11641 taccgggggc cgagcggcga cgattacgag ttttgggacg aggccggcgg acggccgatt
    11701 cagcgagact cggctggtct ggtgctcgaa cggctcgggc tcggctggaa ggagtgtctg
    11761 gcgaacggga tctttccgat cgcgtttgag acgcctcact acggggcgtc cgagaccgat
    11821 tatctcgcca tgcggcaggt gttttcgctt ttcaacgagc gcaccatgcc cacgcctgat
    11881 gtggcctcca tccagtactt cccctatccc gtaatcgacc ggttcgggcg ctatgtggtt
    11941 cctgagaatc tgggctatct gccggcggag aatcccgatc ccagaatttt gatcgagcgc
    12001 gcgcggaata tgcgcgtggt gcgggatgca atagcttcct tttatttcca tccgttcctc
    12061 gatcccgcgc tgcttgccga gacggcgcgc ggcattacag aagcgggctt ccgctttgtt
    12121 tcactgcgtg aattcggcgg cgcggtggat ctcgggggcc attacgccct gcggacctcc
    12181 tccgatcgcg tgcgcctcac gccgcagaag caatactggc ggagccgggg tttcgatggg
    12241 gcggggaagc tggtgagcga aaccgtgtcg gcatcccctt catccggcgc cgtcgaagtg
    12301 gatgtccgcc cgccggccgg cggctgggcg gtcgtggatt gcatagccga ggccccccgg
    12361 cgaacttcta cggccggctg gcgagcccgt ctcgcccagt gggagagccg gctgtggccg
    12421 gcccgccgct tcctgggggg caacggcgat ctggcgccgt cccctcgggc ttgggtgttg
    12481 tggctgtccg cagcctcgcc cgcggaagat aacaaccagc gaagctatcg aaccctgctc
    12541 gaaaccttcg gctaccagac cgccattctt cccctggatc ggttccgggc cgggccagcc
    12601 ggccgcggaa ccatacttgt cgttcccgaa ggcgccgggg agcatctcga accgttccag
    12661 caggacgccg tgctctccta tatccgcgcc ggcggcttgg ccgtgttgga agggcggcag
    12721 aagtggctcg aaccgctcgg cttccgctgg tcaggacact ccataacagt ctccacagtc
    12781 accgatgtgc tttatcccga aatggacttg cgctggaggc cggaggatcg cgtcgaacgc
    12841 ttcacgccgc cggaaggcgc cgaacaattg atggtcgagc cgcaaagcaa gcaattgctg
    12901 gctctggccg ggggatacgg ctcgggacgc tacgtctacc ttgctgtccc gctggatcct
    12961 cacacgcccg gcgcaaccag ccactatcct tattttcccg agtatctggc agaagcattt
    13021 cgcctgccgc cctctccgcg cagcccggga atagaggcgt atttcgaccc gagctaccgc
    13081 gagggcgcca acctggaacg gctcgcggca tcctggcgtc aggaaggtat tcgtacgatc
    13141 tatgcggcgg cgtggcactt ctatcgggac tacgcgtttg actacgagcg cctgatcgct
    13201 gcttgccatc gcaacggcat cgccgtctat gcgtggctcg tgctcccggc gaccacgccc
    13261 ctcatgtggg accagcaccc ggagtggcgg gagcgcacgg cctccggcgg cgccggccgc
    13321 gtgagctggc ggtactccat gaatttacag aacccggcgt gctttcgcgc cgccatggag
    13381 tggatgaacg gcctgctcgc cgggcgcgcc tgggacggcg tcaatatcag cgagctgaac
    13441 ttcgacgccg atttcgaaga ctacctccgc ccggatcgct tcgttccgat gaacgccgat
    13501 gtgcgcgcgg atttccggag ccaagccggc tttgatcccg ttttgctgtt cgctcccgcc
    13561 tcgccctact actacaagcg caatccgcag gcgctcgagc gcttcttgaa ataccgcgag
    13621 gacgtcgtcg caacctggca tgagcgcgtg ctttccgatc tggcgccgct agcccgcgcg
    13681 cgcggctggg aagtgattat caccatgctt gacagcctgc acagcaggta cgtccagcct
    13741 gcgctgggcg tcgattcgcg ccgcatcatc gccttgatga gccgctttga tttcactttg
    13801 caggtggaag atccctcgga gcactggatc gcgccgcctg atcgctaccg gcggttcgcc
    13861 gaaacgtacc gccggttggt tcccgatccg cgccgcctga tgttcgacat caacgtcgtc
    13921 cccgatcgcg gcgtggaggg aacctcgctg ccttcctcta cggccgccgg cgtggagctg
    13981 gcccgcacgg cggctgcggc ggcttcggct tcgggacgcg ccgccatcta ctcggaatac
    14041 acagtcccgg cgcaggactg gcggctgatc ggaaccgccc tggcgggccc cgccgcggtc
    14101 tcggccgcca acgccggctg gcgtctgcat tccccggttc cgctgctgct gggctcgccc
    14161 gccggcgccg agtattatct cgatggccgc ctgtggccgg cgcggctttc cgacggtttg
    14221 ctcgtgcctg cggggcggcg cagcctttcc gccgtccggc cctggtacgc cgccttggac
    14281 cgcggcagcc ctccggcgag gctcgtggac ctcaacggag agctcctcga cgcccgcgcg
    14341 cgccgcacgg gcatcgaaat ccaataccgc agccccggcc gcgcggtcct ggttctaaat
    14401 caaaaaccta gggagatgtt tgtggacggc gccccggccc aagcccagtg ggaaagagca
    14461 tccggcggcg actgggtcgt tgccgctccg cgcggcgagc accggatcgt ggtggaaacg
    14521 tttacccggg cgggcgtgct cgtcaacttc tggggatgga tgtcggcctc cgccatcgcc
    14581 ggcttcggcg ccctcacgac cgcgctgatg gccgtgattt accttcacat ccgcatgcgg
    14641 cgtctggccc ggcgcggagg cctgtcttga ccggcgccgc gctagcggcc tgcctcgcga
    14701 ctctcgccgc ggccatccca ggagcggctc agacgccatc cgcgccgcgc cgcgtctccg
    14761 ctccgatttc tttctatgat gacgccgatc ccacagatcc cggcgtgctt tcggccagcg
    14821 cgtcgttctc ctacacccgg gtggacgccg ggcacgatct cgacggcccg tcggcctacc
    14881 tgagcctggg gctgcacaag cgtatcgaag tgtccgcgga tctggggagg gccgagtcgc
    14941 tgttcgaaga gacccgcatt tccggcatag ccgattcgta cctcgccgcc aagtttttag
    15001 tttttccgga gggccgccgc cgcccggccc tggccctgaa gcccatgctc gagattctcg
    15061 gcccagccag cattcaccaa gacgtgctgg cccccggccg cgtgaacttc gtcccttcgt
    15121 tcatcctgca aaaatcgttc gatgacttcc gggtctacta catgggcgga tacgtcaccc
    15181 ggggcattct gttccactct ctcgccggtg aattgaacct gtggagccgc gtgacgccga
    15241 cagtgattct ttcggccgcg agagtgaccc gcgaagcggc cttgatcagc gatttgggtc
    15301 tgaaccggtc tcgcgcggac gcgacggtca gcctcaccat catgttgacg ccgcgatgga
    15361 gcgtcttcgc cggcggcggg cgcagcttcg gacgctctga tctgaatagt tcgtgctacg
    15421 cgttcagcgg cggcgcggct ttccattttc gcctctgggg tgaaaaatga gcagaaattt
    15481 cttcctcgcc gcggccctgt ctgtgatgcc aatgctcgcg cagggggtcg cctacattgt
    15541 ggacaatagg aacggccagg tggatgtatt cgatctgggt tcttggcgag tgatttccag
    15601 tattcggacc gggaatcagg ccggcgagat ttacattctg cccaacaacc gtttcgcgtt
    15661 tgtttccaac cagggcgaca gcaatgtcac gctgctcgat ctccaggccg gcgccttcgt
    15721 gacctccctt cccaccggcc aaggtcccgg cagtctcgcc ggcacgcccg acgggcgcct
    15781 gctctatgtg gccaacgagg gcagcaacga cgtcacggtt atcgacgtcg cccggcgcgc
    15841 cgtcgtcgcc accattcccg tgggcacaac gcccgtgcag gtgaacctgt ctcctgacgg
    15901 gctgttcgtc tacgtggtca atcaagatga cgggacgatc accgtgatcg acgcgattcg
    15961 caatcaggtg aaacagactt tgcctgtcgg ccagcagccg aatcagatcg cgatcgccgc
    16021 caatctgaac actgcctaca tcctgaatac agggtccaac agcgtttccc ttctcgactt
    16081 gagtactaat accatcaccg gctcggttcc ggtgggcgcc ggcccggtca ccgcggcgtt
    16141 ttcttccgat gggcagcgcc tttttgtggt caaccgcgat tcgaacagcg tcagcatcat
    16201 cgatacccgg caaaaccgcg tgatcgctac aatcggcgtg ggggcgcggc cggttgcgat
    16261 cgccgtcacc ttcgacggca agttcgctta tgtgagcaat cagggagacg gcacggtaag
    16321 cctgctggac ctcaccgcga acgcccaggc cgacgtcatc tcggtcggtg gcagcccgtt
    16381 cagcgtgcag tttgatccca atgagaactt cctctacgtc accaacctga cttccggcag
    16441 catcagcgtc attgatacca gcaccgacaa acccacttcg gtaccggtgg gcggcgcgcc
    16501 ggtgcagttt gcctttctga acgctcctac gctcctcgaa atttccccca accccgcgcc
    16561 ggtttgcagc ctgatcacgc tgaaagggga aggatttgta ccggcctcgg ccgtttggta
    16621 caacaggtcg gcgtttgccg gcgccgcctc ggcgtgcccg gcggctcccg cccctgcggg
    16681 ttttgtggat agcgagacgt ggcaggcaat tgttccgcca ctggccagct cgagcgcggg
    16741 catttccgta gtgaaccccg atggcaatgc ttcggaacaa ctggttctcc agatcgcgcc
    16801 gagcccttcg ccggtgatca tttcgcctgg aggcgtcgtc gagggggccg gcttccagaa
    16861 ggcgccctat ccgatcagtg gaggctcgat cgtctcggta ttcggaagct tccccggagc
    16921 ttccacggat caagcccgga attttccgct tccgactact ttggccaacg ttcgcgtcga
    16981 cttcaacggc gtgccggcgg cgctgcttta tgcgagcccg gcgcagatca atgccgtcgc
    17041 gcccgccggc cttttggcgc tggcggcagg tctcggcaac gggcaagtaa cgccggtcca
    17101 ggtctcggtt acggttctgg gcccgcccgg ctcgcctccc tacgtcagcg ctccggcgcc
    17161 ggtcagcgtc gcggccgccg ctcccggcat attttcggat ccggcgagcg gcatcggcgc
    17221 cttcctgcat ggcgccgatc tttcgcctgt gaccaccgcg aatccggctc gcgccggtga
    17281 aacgatcgtg ctgtactgca cggggcttgg caacaccagc ccccctccga tggacggaca
    17341 gcctgcgccc gccgatatcc tttctccagc cgccagcctt cctgtggtaa cggtgggcgg
    17401 cgcacaggcc aatgttcgtt tcgccgggct cgctcccctc tatgcgggcc tgtaccaaat
    17461 taattttgat gttccttccg gaacgcccgg cggcgccgcg atggttaagg tcacctccgc
    17521 atcgtggacc agcaacggcg tcctgctgcc cacacggcca tagcacgtgt actccagtat
    17581 tgttcttgac attctgttta tcgtcgccgt cacgctgatc tggttcatgc tcgcctacca
    17641 gtcgttgctt ttcttcctcg gccatcgcta ttaccggctg acccggcgaa gcacacgccc
    17701 gcccagggcg gcgctccctg attcggcgct gccctgtatt tcacttctgc tgccgtgccg
    17761 taatgaagag cgcgtgatcg cccgaacgct gcgcgctctg ctggcgctcg actatcctgg
    17821 cgacaaactg gaaattctgg tgattaatga cgccagtgag gaccggaccg cggaaatcgc
    17881 gcgcacattc gccagcgacg cccgcgtgcg gctcctcgac gtgcccatga ccgaggccgc
    17941 acgcggcaag gccgccgctc tgaacttcgc gctccgtcac gcccgctacc ccgtgctggc
    18001 catctatgac gccgataata cgcccgagcc gggcgctctc cgcccgctcg tcgagcagtt
    18061 gctcgccgat ccctccctcg gggccgccat cggcgtctat cgcgccgtca atcgccgcag
    18121 agcgctgctc acgcgctttc tcaacatcga aggcatcgga ttccagtgga tcatccaggc
    18181 cggccggtgg atgctgatgc gccttaccac tctgcccggc accaattacg tgatccgccg
    18241 cgaactggtc gaatctttgg gcggctggga cgagcaggcg ctgacggaag acgccgagtt
    18301 gaccatccgc gtgtaccagg ccggctatct gattcagttc gtgcctgctt ccgtcacctg
    18361 ggaacaggaa ccggaaacca tccgtgtgtg gttccgccag aggcaccgct gggtgcgcgg
    18421 ctataactac ctgttccgga aatacgccgg gaagctgctc caggctcgcc cgcgccgcgt
    18481 gggcctcgag ctgctttact cgctttcgct ctactacgtt ttttttctgg ccatcgtcat
    18541 ttccgatgcg ctgttcgtgc tgagctccgc acgcgtgatt cacatcggcg tgccgggacc
    18601 tttcagcatt ctgtggctct tcgcctatgt gacctttgtg ttgcagcttg tcatcgcgct
    18661 ctcctgcgag ccgggagagg attcgctgct gaacattctg ctcacgatgg tgatgtattt
    18721 ttcctattgc cagctttgga tccctgtggt tgctcgcgct ttcttcgatg acttcatcgt
    18781 gcggcgcgaa atcaagtggg cgaagactga acgctatgaa gtacgttgaa gccgcgcggc
    18841 tggggtcgaa caaatccggc ccgcactcta gccgaacggt ggcaaaatag aaacgatggg
    18901 caggtgcggg aagccggccg ccagcaaggc cgcggcgctg ctgttgctgg cgggttcttt
    18961 aacgcggttg cagccctccc cccatggctg tccttcgggt gcgcccatcg ccaccttcca
    19021 gctttccgta gagaatactt cgagcgagcc gcttctgccc gtgcggtccg tgagccagat
    19081 ccggtccgga cagcatattt tttacgagcc gctcgatctg actgcgagtc tcaagaagag
    19141 cgggaagatc gctctgattc tggcgcccgc cggcctgccc gcctccggtc atttgaccgt
    19201 gctcgatccc aaaccggccg cccagccggc ccaatgggcc gttccttatc aaaccgaagt
    19261 gctggggctt gtggtgggtc cgcagggctt agacgtcgca aaagtttcct ctcttctgga
    19321 ccaagatcat gagctgatgt cgcaattggc cgactacgcg gctcaatcta cccaggtcga
    19381 atcgctcatc gaaaaacttt cctcggccga acaggagacc gacaagggcg cggcactcag
    19441 cgcggctctg ggcggcctcg cgggctcatc gccctccaag ctggactcga atgcctccgc
    19501 cgaggcgcag gcgatggcgc tgctgcgtgc gctcaacccg gcggtggggg cttacaatcc
    19561 gctcgcgccg cagccggcgg cccgcatgca gcagtctgcc ggcttggcag cctctttggc
    19621 cggccttttt ctcggaaatc cggtggggtt ggccgtggac ggtgcggcta tgctcaacaa
    19681 tcttagaact ctgttgttcc ccggcaccga ttttcgcagc gccctggccc ggccggcttt
    19741 ctccgacggc atgactctgt gcgccgcgca gggagacgtt aaatcgagag cccgcctggc
    19801 atacctctgg gcgctccggc ttcccaacga cgtcgcgccc gccatatctg tcggagactc
    19861 cgtccatctg ccgatcggcg tcaagtgggc tgtgcctgtg aaatgggaag gcgcgtccaa
    19921 atttccgccg ctggagcgcg tccgcgagtg gaccctggtt ccccaggacg actccgccgg
    19981 gattcccgtc accgtgaagc cgctcccgca gttccgctcc ctcgaggtcg acctgagccg
    20041 cttcagcggc ccgcccggcg tctatcgcct cactggcaag tgggattggg accggcttga
    20101 cgtcgccggg tctttccggt tgttctccct gagcacgctc gagaaggcgc gcctgacgga
    20161 ggcctcgcaa gaccggctcg tttccggaag cggccaggcc gctatcgagc tcgaaggcgg
    20221 cgatttccag tttgtccaat cggtggggct gcgcgacgcc gcccggccgg gcgcgaagcc
    20281 ggccgaactc acatttcaca cgctgcccct gcgcggcgcc gcagcgccgg caaatctcga
    20341 ggtcctggtc aacctgcggg aatggaaggc cggagagtat cagttattac taacccagcc
    20401 ggatgggtcc ataca
//
LOCUS       DASREK010000242         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1274554, whole genome shotgun sequence.
ACCESSION   DASREK010000242 DASREK010000000
VERSION     DASREK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274554"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VFA54_12890"
     CDS             complement(<1..928)
                     /locus_tag="VFA54_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HZS51755.1"
                     /translation="MLDTAAAKTASDTGFFGHPRGLSTLFFTEMWERFSYYGMRAILI
                     YFMTDQVAKGGLGFSDAKAGAVYGLYTAMVYLLCLGGGWVADRISGQRRAVLIGGLFI
                     AGGEFCLVAPSELSFYSGLVLLMAGTGLLKGNVSTIVGQLYAPGDPRRDSGFSIFYMG
                     INLGALISPIICGYVGERISWRLGFGVAGLGMLAGLVQYLLGSRRLGSAGLHPPGTGD
                     PEQDRQRRRRAILAVGCGLAVFALLGALGAAGVIGFTATSISNGLGWGLLGISVVVFW
                     WLIFSKGWSLEERKRSAAILVLFIASALFWASF"
     gene            complement(928..1101)
                     /locus_tag="VFA54_12895"
     CDS             complement(928..1101)
                     /locus_tag="VFA54_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51756.1"
                     /translation="MPVKARPWKAAARLRVQPRAPRERVAGVAGETSRHKRIEIDGVS
                     QDDLNRFLQERVR"
     gene            complement(1092..>2320)
                     /locus_tag="VFA54_12900"
     CDS             complement(1092..>2320)
                     /locus_tag="VFA54_12900"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51757.1"
                     /translation="DVIAVHNVSKLYRLYDRPADRIVEALSWNRRRRHKDFWALRDIS
                     FAVERGETLGIVGPNGCGKSTLLQIICGVLEPTAGRVVKQGRVAALLELGAGFNPEFT
                     GRENVYLNGEILGLPRAEIERVLPEIERFADIGEFIDRPVKTYSSGMYVRLAFSTAIN
                     VSPDILVVDEALAVGDAVFANRCVRKFEEIKKRGVTVVFVSHDLGLVKLISDRAIFLL
                     NGRIEAEGGPGHVINRYIGVVLERQKAFDLAERPPSLLPATHRHGDRMAEVIAVELFG
                     REGRPALVLETGEPVTVRVTSRFYADQPDPMVGIMIRTRIGMDVFGTNTKLEKLSLGP
                     CRAGDTLEVDFHFNCWLAPQEYTLTVATQHPDGSSHDWLDGVLAFSVVAERYTAGVAN
                     LHARAVARRFSEQPCR"
BASE COUNT          498 a          764 c          657 g          401 t
ORIGIN      
        1 cgaacgacgc ccaaaataaa gccgacgcaa taaataagac cagaatggct gccgagcgct
       61 tccgttcttc cagcgaccag cctttcgaaa aaatcagcca ccagaaaacc accaccgaaa
      121 ttcccagcag cccccagcct aggccgttcg aaatcgaggt ggctgtaaac ccgatgacgc
      181 cggcggctcc gagtgcgccc aggagcgcaa aaaccgccag tccgcaccct actgctaaga
      241 tagctctgcg tcttctttgc cggtcctgtt cgggatcgcc ggtgccgggc ggatgcagtc
      301 cggcagaccc tagccggcgg cttcccagaa gatactggac aagccccgcc agcatgccca
      361 atcccgccac gccgaatccc agacgccaac tgatgcgctc gcccacatac ccgcaaatga
      421 tcggcgaaat gagggcgccg aggttgatac ccatgtagaa aatggagaaa ccggaatcgc
      481 gccgcggatc acccggcgca taaagctgtc cgacaattgt gctgacgttg ccttttagca
      541 ggcctgtacc cgccatcagc agcacaagac cgctataaaa agaaagttcg ctgggcgcca
      601 ccaggcaaaa ttcaccacca gcgatgaaaa gcccgcctat cagaacagca cgccgctgcc
      661 cgctgatgcg atccgccacc catccgccgc cgagacacaa tagatagacc atggcggtgt
      721 agaggccata gacggcgccg gccttggcgt cgctgaaccc cagcccgccc ttggcaacct
      781 gatcggtcat gaagtagatc aggatggctc gcatcccgta gtagctgaac cgctcccaca
      841 tttcggtgaa aaacagtgtg gagaggccgc ggggatggcc gaagaagccg gtgtcgctgg
      901 cggttttggc ggcggcggta tcaagcatca gcggactctt tcttgaagga accggttcaa
      961 gtcgtcctgc gaaactccat caatttcgat gcgcttgtgg cggctggttt cgccggccac
     1021 gccggccacg cgttctctcg gcgcacgcgg ctgcacacgg agccgcgcgg cggctttcca
     1081 gggtcgcgcc ttcaccggca tggctgctcc gaaaaacgcc gcgccacagc ccgtgcgtgc
     1141 aggttggcca cgcccgcggt atagcgctcc gccacgactg agaaggccag cactccatcc
     1201 agccagtcgt ggctcgagcc atccgggtgc tgggttgcca ccgtcaaagt gtactcctgc
     1261 ggggccagcc agcagttgaa atggaagtcc acttccagag tgtcgccagc ccggcagggg
     1321 cccagcgaca gtttttcgag tttcgtgttg gtcccgaaca cgtccatccc gatccgcgtc
     1381 cggatcatga tgcccaccat cggatccggc tgatcggcat agaagcgcga cgtcacccgc
     1441 acagtcaccg gctcgcccgt ttcgagcacc agcgccgggc gcccttccct gccgaatagc
     1501 tcgacggcga tcacttccgc catccggtcc ccgtggcggt gcgtggcggg gaggagcgaa
     1561 ggcggcctct cagccagatc aaacgccttc tgccgctcga gcacgactcc gatgtatcga
     1621 ttgatgacat ggccaggccc gccttctgcc tcgatgcgcc cgttgagcag gaaaatcgcg
     1681 cggtccgaga tcagtttcac caggcccaga tcgtgggaaa caaaaacgac cgtcacgccg
     1741 cgctttttga tctcctcgaa tttgcgcacg caacgattgg cgaatacggc atcgcctacg
     1801 gccagagctt cgtcgaccac cagaatatca ggcgaaacgt taatggcagt cgagaatgcc
     1861 aaccggacgt acatgccgct cgagtaggtc tttacggggc ggtcgataaa ctccccgatg
     1921 tcggcgaaac gctcgatttc cggcaagacg cgctcgattt cagcacgcgg caggccaaga
     1981 atctccccgt tcagataaac attttcgcgg ccggtgaact cggggttaaa accggcgccc
     2041 aattcgagca gcgccgctac ccgtccttgc tttacgaccc gtcccgccgt aggttccagc
     2101 accccgcaga taatttgcag cagcgtgctc ttgccgcatc cgttagggcc gactatgccc
     2161 agggtctcgc cgcgttcgac ggcaaaactg atgtcgcgaa gcgcccaaaa atctttgtgc
     2221 cgccggcggc ggttccagga caacgcttcc acgatccggt ccgcgggccg gtcataaaga
     2281 cggtagagct tggagacatt atggacggcg atcacgtcag
//
LOCUS       DASREK010000243        13861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_448864, whole genome shotgun sequence.
ACCESSION   DASREK010000243 DASREK010000000
VERSION     DASREK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13861
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448864"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /gene="mraY"
                     /locus_tag="VFA54_12905"
     CDS             complement(<1..1116)
                     /gene="mraY"
                     /locus_tag="VFA54_12905"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZS51758.1"
                     /translation="MLYYLLYQRLFPHFSPFRVFGYITFRTAYASLTALTICLVLGPW
                     LIRKLKEFQIGQIIREEGPKSHQVKQGTPTMGGLLINISILVPTLLWADLTDVYVWIA
                     IFALVGFGAIGFIDDYSKLRRKHNLGLTGKRKLLLQVLVSFAIGIALLVLQKHGLYST
                     RMNFPFLKFYRPDLLIDSLLANPYTYPLAFVFFFLFLVVVLVGCSNAVNLTDGLDGLA
                     IGLTVIAAGALTVLTYASGHAQIATYLEIERVSRAGELTIFGGSMVGASLGFLWYNAH
                     PADIFMGDVGSLALGGAIGTVAVLVKQEILLLFIGGVFVIEALSVIVQVASFKLRGKR
                     VFKMAPLHHHFEQLGWTESKIIARFWITGLVMALFALT"
     gene            complement(1129..2550)
                     /gene="murF"
                     /locus_tag="VFA54_12910"
     CDS             complement(1129..2550)
                     /gene="murF"
                     /locus_tag="VFA54_12910"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZS51759.1"
                     /translation="MKLPLREIAQMTGAAIPEDESLQSAVAEGYSIDSRTVAPGEIFF
                     AVKGPNFDGHDFIEAALARGAVAAVASRAPQRLRERVLLVPNPRSALQQLAAQARQRW
                     GGRIVAITGSNGKTTTKEVTAALLSTRFRVAKSEGNLNNDLGVPLSLLRIPDQSEVGV
                     LELGMNHAGEIRSLAAIARPDVGVVTNVNAVHLEFFESVDGIALAKRELIEALPRGGT
                     AVLNADDSRVLGFAQIFQGRVLTFGIDQMADIRALDVRDQGIQGSRFRLEQNGPEFLT
                     PLPGRHNLYNTLAGIAAAQALDVPAGSLGDAVRKLSAVRMRGEILEIGEIRVIDDCYN
                     SNPRAAEAMLDLLAAIPARRHVAVLGQMLELGTDGPQLHRQVGRKAAQAGVDLLVGVG
                     GAARQLVDQAIDDGMPESAARFFADAQTAGEFLQLAVRPGDLILFKGSRGVKLERALE
                     AVRNRFSEMSKEAALGGSLMPKG"
     gene            complement(2547..4043)
                     /locus_tag="VFA54_12915"
     CDS             complement(2547..4043)
                     /locus_tag="VFA54_12915"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZS51760.1"
                     /translation="MTLDQMLEGVETPLAAPSVPVFGLQYDSRKLKPGEVFFAFPGEH
                     VDGHQFISQAVGKGAAAIVSERPPGSGGALPEQIPWVQVRHGREALALAALNFYDHPD
                     RRLRLTGVTGTNGKTTTVYLIDSILRAAGFTTATLGTIEQTIAGEHRPTANTTPESLD
                     LIRLFAELLDRGGSHVTFEVSSHALELRRVYGLQFHTAVFTNLTRDHLDFHGTIENYA
                     AAKRRLLEGTGGPPPRFAVIHHEDPWGRKWEKLGGFHLLSYGLNSGACITASKVVSSF
                     EGLRFTANTPGGPIPIRSPLLGRFNILNILAAVGVGLSYDLSPEVIAAGVAECKAVPG
                     RFERVDAGGPFVVIVDYAHTDDALRNVIRAARELVSDRNPPGRVITLFGCGGDRDRTK
                     RPLMGEAAGSLSDFVVLTSDNPRSEDPLNIINDVLVGLQRATAPYRLEPDRAAAIKIA
                     IETARAGDIVILAGKGHETYQIIGSSVNPFDDREVARQVLRGMGYDHR"
     gene            complement(4053..6149)
                     /locus_tag="VFA54_12920"
     CDS             complement(4053..6149)
                     /locus_tag="VFA54_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HZS51761.1"
                     /translation="MDPIFDPAMAMRRRKVVWFSIVALAWAAVVFSRLVYLQIIRRGF
                     FEERARLQQQRAIPVRASRGLILDRRGRELAMSMPVDSICAMPAEISDPEGVGTILAR
                     ILGIPRNPLIKKLSTGHGFCWIARMVDPELADRVRALDLRGIYFQKESKRYYPKGSTA
                     AHLIGAVGLDQTGLAGIEQARESELEGRPGLMLVSTDARQKQFDGRLSQQPDPGTNVI
                     LTIDERIQYVAERELNAAIERTAAAAGSIVVLEPSTGALLAIANYPTFNPNQPVRSPA
                     DVEKRRNFAVSSSFEPGSTFKLVTLSAALEEKVARPTEVVDCQMGSIVVAGHRIRDHK
                     PFGNLTVEQILVNSSDVGAIKMGLRLGDNRMYEYMRRFGFGQPTGIELPGEARGLTKP
                     PKQWSKISIGAISMGQEVGVTAIQLVQAVGAIANGGVLAPPHIIEATFEDGKPPVKMQ
                     HPPARRVISQETAAEMRHMMEMVVLQGTGKLARLEGYTAGGKTGTAQKIDPRTGAYSK
                     TNFVASFVGIAPVNNPAIVVAIILDSPRGMHMGGSVSAPVFPRVAEEVLRYLRVPQEL
                     PIDPAVRRRRGAPGSQPDPKLLAEVTDFTPDNGLTSSPPPKFQKAMPHGIAANEAQSE
                     LREPSRSVGSPVLLAVSASLMPDFYGKPVREVVEQATAIGLAVDLKGSGVARRQSPSP
                     GSPLFPGVRAMVEFER"
     gene            complement(6251..6796)
                     /locus_tag="VFA54_12925"
     CDS             complement(6251..6796)
                     /locus_tag="VFA54_12925"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZS51762.1"
                     /translation="METQTGAPERHDSRPYKGFFYDGVAAGREVRAGLSRRPAATRPL
                     PMEDLCFFVKAIDNSRLVRVVDPNSRRECLGLIGCVSAFFFLALLYIVPSLAILRTGY
                     RLQDLKKEHEALVETSRQLQVKEATLRDPQRIYTIAKGKLGLDEPVPEQVIWPEADGN
                     LPTGREMLARNSPVTIRQRAR"
     gene            complement(6809..7693)
                     /gene="rsmH"
                     /locus_tag="VFA54_12930"
     CDS             complement(6809..7693)
                     /gene="rsmH"
                     /locus_tag="VFA54_12930"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZS51763.1"
                     /translation="MAHIPVLLNEAIEWLRLSPDGIYVDCTVGEGGYAAAIAGRLRGG
                     RLIAIDRDPRAIEAARQRLAAFGDRVMLRRSRFSELPQILQQVGVEAVDGIVADLGVS
                     RGQLEAPDRGFSFLTDGPLDMRMDPDQTLTAERIVNQYGEKPLSDLIYRYGEERRSRR
                     ITRAIVRARPLTGTRQLAEVIKRAAPRTGGGRIDPATRTFQALRIAVNDELGELERLL
                     ESAPPLLKPGGRFVIISFHSLEDRAVKQGFQRWAQRHLLRILTKHVIRPSEEEVRANP
                     ASRSAKLRAAERTTAEEE"
     gene            complement(7714..8160)
                     /locus_tag="VFA54_12935"
     CDS             complement(7714..8160)
                     /locus_tag="VFA54_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51764.1"
                     /translation="MFRGTATVKVDDKGRLKLPAFVKERLDERYGANSIYFVTSITGD
                     IVLIYPISEWERIEQTLSQAPQFDKLKKKFLFQANQYGAEASLDEQGRLLIPAKLRDQ
                     AGMKGEVKLNWETNHIEVLSQARYEEEAERNRLTPEDFDNLAKLGV"
     gene            complement(8418..9380)
                     /locus_tag="VFA54_12940"
     CDS             complement(8418..9380)
                     /locus_tag="VFA54_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS51765.1"
                     /translation="MTTREHAAVIVLLLALEVPLFLWARPQAASSGQPAGAAERATGD
                     FKISMDVNLVVLGVAVRDHGGGSVSGLGRDCFRVYENGRPQEIKLFEPEDVPVTIGLV
                     VDGSGSMMPKRRETIMAALGFLQYSNPDDEMFVVNFNEKVFLGLPPESPFTNERSQML
                     TALMRFPPAGRTALYDAIATGLKQLERGRRQKKILVVFSDGGDNASRASYADVLAAAE
                     QSNVIIYTIGLFDDQDPDRNPKVLKKLAHLTGGEAYLSREHRDLVGICKRIAHDIRSQ
                     YTIGYAPSVADGSYHSIKVTAETPDHKKLSVRTRTGYRTEPHKD"
     gene            9805..11007
                     /locus_tag="VFA54_12945"
     CDS             9805..11007
                     /locus_tag="VFA54_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZS51766.1"
                     /translation="MPHRLNRRQWIMGCASLALLRGAERPRLKITDMEVFVVKVNVRG
                     NWIFVRLKTDLGLTGLGEASHGGGFQGEGDAQMPTQLAEYFGLVRGGSPFEIEAYRAR
                     GRARAKAGGRLAATAFSAIEQAQWDLAAKALGAPLYELFGGKLRSELDVYANINRATN
                     EDRSPAGFAANASKAVEEGFRAIKAAPFDDFPKLDAPAGERQKFAELGIARVEAIRRA
                     VGPHPNLLIDCHSHFDPALGIEVARRLEPQNLYWYEEPVPPERIEESAAIRSKISQRM
                     AGGELLFGLEGFAPVCRRRSMDIIMPDVKHCGGILEALKISVLAEADGVLVAPHNPSG
                     PVATAASVQLCAGLPNFLTLEYAWGEAPWRKDLIIPPEEFSNGKIRVSDRPGFGIELN
                     ERLAREHA"
     gene            11150..12649
                     /locus_tag="VFA54_12950"
     CDS             11150..12649
                     /locus_tag="VFA54_12950"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HZS51767.1"
                     /translation="MPAHVNFLAFDLGAESGRAMLGRFDGARLAFEEVHRFPNSPVRV
                     LDSLHWDVLRLFGELKRGLAAGAKQAGGGLASAGVDTWGVDYALLGADGALLENPYHY
                     RDSRTDGVMEKLLATVPREEVFERTGIQFLRLNTLYQLYALKLQRPHVLERSHTLLMM
                     PDLFHYWLTGRAVSEYTIATTTQFYDPRCGLWARDLLEKLHLPVRILPGIVPPGTQLG
                     PLLPSVAAEAGIQPMPVIVPACHDTGSAVAAVPAQPGGDWAFLSSGTWSPLGVELPAP
                     IINGAALALNFTNEGGAWTTTRLLKNLTGMWLFEECRREWAREGMPTGYQGLCEAALA
                     ADPFRSIIDPDDPMFLTPGGMPGKIVTFCRRTGQPVPEDQGAFTRAIFESLALSYRSA
                     LDELRQILGRNLRTLHIVGGGSRNPLLCRFTADATGCEVLAGPEEATAIGNVLLQAMA
                     LGYVGTPVEARDLVRRSFPPKRYDPARRERWNEAAARRRDLITRAAAAS"
     gene            complement(12624..13574)
                     /locus_tag="VFA54_12955"
     CDS             complement(12624..13574)
                     /locus_tag="VFA54_12955"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS51768.1"
                     /translation="MKRARDPHAALGAVLVLILTAGSAAAQQPKHINKAIDLLSQGQP
                     IYYTSGHGGYEEGVKMAQTWADYITYDMEHSPFDVAKLADFMRGLVKGGPTKSGHRTP
                     AVIVTLPTDGTDEATMRANAWMVKQVLATGIHGILLCHADDPGAVRAFVESARFPFQH
                     LGVNEGLGEGRRGAHGTASAARIWGISPKEYAQKADVWPLNPNGEIMLGLKIEDKRAL
                     ANVDQSLKVPGLAFAEWGPGDMGYSLGFPENHDPPYPPEMIAARAKVLAACKANKLAF
                     LNQVRPNDVIDMIREGVMIGASGPEAAEIGRKFTKRPQPW"
BASE COUNT         2652 a         4268 c         4186 g         2755 t
ORIGIN      
        1 cgtgagcgca aatagagcca tcaccaggcc ggtgatccaa aagcgggcga taatcttcga
       61 ttccgtccag cccagttgct caaaatggtg atgcagcggc gccattttga agacacgctt
      121 gccgcgcagc ttgaagctgg cgacctgcac gatcaccgaa agagcttcga tcacgaatac
      181 gccgccgata aaaagcagga ggatttcctg ctttactagc acagctaccg tgccaattgc
      241 tccgcccagc gccagggacc cgacatcgcc cataaaaata tccgccggat gggcgttgta
      301 ccaaagaaag cccaggctcg cccctaccat ggagccgccg aagatcgtta gctcgcccgc
      361 ccgcgagacg cgttcgatct ccagatacgt ggcaatctgg gcatggcctg atgcatatgt
      421 cagcaccgtg agggctccgg ctgcaatcac cgtcagcccg atcgcgaggc cgtccaagcc
      481 gtccgtcaag ttcactgcgt tggaacatcc cacgagtacc accaccagga acagaaaaaa
      541 aaacacaaaa gcgagcgggt acgtgtaggg attcgccagc agggagtcga tcaaaagatc
      601 cggccggtag aacttcaaga acggaaagtt cattcgcgtc gaataaagcc cgtgcttctg
      661 gagcacgagc aaggcgatgc cgatggcgaa gctgaccagc acctgaagca acagcttccg
      721 cttgcccgtc aggccgagat tgtgcttgcg gcgcagcttg ctgtaatcgt cgataaaccc
      781 gatcgcccca aaaccgacga gcgcaaaaat ggcgatccaa acgtaaacgt cggtgaggtc
      841 cgcccacaac agcgtaggaa ccaggattga gatattaatg agcaggccgc ccatggtcgg
      901 cgtgccttgt ttcacctgat gggatttggg tccttcctcg cggatgattt ggccgatttg
      961 gaattccttc aatttccgga tgagccaggg gcccagcacc aggcaaatgg taagcgcggt
     1021 caggctcgcg tacgccgtgc ggaaggtgat gtagccaaag acacgaaacg ggctgaaatg
     1081 gggaaacagc cgctggtaca gaaggtaata aagcataact gcagcacgtc agccctttgg
     1141 catcagcgac cccccgagcg cggcttcctt gctcatctcc gagaaccggt tgcgaacggc
     1201 ttccaaagcc cgttccagct tcactcccct cgatcctttg aacaggatga gatcccccgg
     1261 gcgaaccgcg agctgcaaaa actctcctgc ggtttgcgcg tcggcaaaaa accgcgcggc
     1321 gctctcgggc atgccgtcat cgatcgcctg atccacaagc tgccgcgccg cgccgccgac
     1381 cccgacgagc aggtcaacgc cggcctgcgc ggctttccgg cccacttgcc ggtgcaactg
     1441 cggaccgtcc gttcctagct ccagcatttg gccgagcacg gcgacgtgac gccgggcggg
     1501 aatcgcggca agcaggtcga gcatggcttc agccgcccgt ggattcgagt tgtagcagtc
     1561 gtcgatgacg cggatctctc caatttccag tatttctccg cgcatgcgga ccgccgagag
     1621 tttgcgcacc gcatccccca aggatccggc cgggacatcc agagcttgtg cggcggcgat
     1681 gccggccaga gtgttgtaga ggttatggcg gccgggcagc ggcgtcagga actccggacc
     1741 gttctgctcc aaacgaaagc gcgatccctg gatcccctgg tcgcgaacat cgagtgcgcg
     1801 aatgtccgcc atctggtcta tgccgaatgt caggacgcgg ccttggaaaa tttgagcgaa
     1861 tccgagcacg cggctgtcgt cggcgttcag cacggcggtc ccgccccggg gcaacgcctc
     1921 gatcaactca cgtttcgcga gggcgattcc gtctacggac tcaaagaact ccaggtgtac
     1981 ggcattgacg ttggtcacca ctcccacatc cgggcgggcg atggcggcga gggaccgaat
     2041 ttcgccggca tgattcatgc ccagctccag gacgcccact tcgctctgat cgggaatccg
     2101 cagcagcgaa agaggaaccc caagatcatt attcagattt ccctcgctct tcgcgacgcg
     2161 gaagcgggtg gagaggagtg cggccgtaac ttccttggtt gtagttttcc cattgcttcc
     2221 agttatggcg acaatccggc cgccccagcg ctggcgggcc tgggccgcaa gctgctgtag
     2281 ggcgctcctg gggttcggta cgagcagtac ccgctcgcga agcctctgcg gagcgcgaga
     2341 tgccacggcc gccaccgcgc ccctggccag tgctgcttca atgaaatcgt gcccgtcgaa
     2401 gtttggtcct ttaacagcga agaagatctc tcccggggcg acggtcctgg agtcgattga
     2461 atagccttcc gccaccgcgg attgtaaact ttcgtcttcc gggatggccg ccccggtcat
     2521 ttgcgcaatt tctcgcagag gaagcttcat cgatggtcgt agcccatccc tctaagcacc
     2581 tgccgggcca cctcccgatc gtcgaacggg ttcacgctcg agccgatgat ctgatacgtt
     2641 tcgtgcccct tgccggccag aatgacaatg tccccggcgc gggctgtttc gattgcgatc
     2701 ttgatcgccg ccgccctatc cggctccaga cgatagggag ccgtggcgcg ctggagccct
     2761 accaaaacat cgttgatgat gttgagcgga tcttccgatc tgggattatc cgatgtgagg
     2821 accacgaaat cgctcaggct gccggcggcc tcgcccatca gcggcctctt ggtgcggtct
     2881 cgatcaccgc cgcacccgaa caaagtgatg acgcgccccg gcgggttgcg atcagagacc
     2941 aattcgcgcg ccgcccggat gacattccga agcgcatcat cggtgtgagc gtaatcgaca
     3001 atcacgacga acggcccgcc ggcatctact cgctcgaagc ggccgggcac agccttacac
     3061 tcggctaccc cggcggcgat gacctcggga ctcagatcgt aggaaagccc gaccccgacg
     3121 gccgccagga tattcagaat gttgaatctt cccagcagtg gggagcggat tggaatcggc
     3181 ccgcccggcg tattggcggt gaaccgcagc ccctcgaagc tcgaaactac cttggaggca
     3241 gtaatacacg cgccgctatt caggccatag ctcagcagat gaaacccccc tagcttttcc
     3301 cacttgcgtc cccaggggtc ctcgtgatgg attacggcaa accggggcgg cggtccgccg
     3361 gtcccttcga ggagcctgcg tttggccgcg gcgtaattct caattgttcc gtgaaagtcc
     3421 aaatgatccc gtgtcaggtt cgtgaagacc gccgtgtgga actgaaggcc gtagacccgg
     3481 cgcaattcca gggcgtgcga ggatacttcg aaagttacat ggctgccgcc gcggtccagc
     3541 agttcggcaa agaggcggat gagatccaga gattccggcg tcgtgttggc ggtgggcctg
     3601 tgttcgccgg cgatggtttg ctcaatggtg ccgagcgttg cagtggtgaa acctgccgcc
     3661 cgcagaatcg aatcgatcaa gtagacggtt gtggtcttgc cgttcgtgcc ggtaacccct
     3721 gtcaagcgca gccggcgatc ggggtgatcg taaaagttga gggccgccaa agccagggct
     3781 tcccgcccgt ggcggacctg aacccaggga atctgttcgg gaagcgcgcc gccgctgccg
     3841 ggcggccgct cgctgacaat agccgccgcg cccttgccga ccgcctgcga gatgaattga
     3901 tggccgtcta cgtgttcgcc agggaaagcg aaaaacacct cgccaggctt caatttgcgg
     3961 gagtcatatt gtaagccgaa gaccggaacc gagggcgccg ctaacggcgt ctccacgcct
     4021 tccagcatct gatccagggt catgttccag ggtcaccgct cgaattccac catggcccgc
     4081 acgccaggaa acagcgggct gcctggagat ggcgattggc ggcgggctac cccgctgccc
     4141 ttcaaatcga ccgccaggcc aattgcggtg gcttgctcca cgacttcacg gactggcttt
     4201 ccgtaaaaat cgggcatcaa agaggcgctg acagccaaca gaaccggcga tcccacactg
     4261 cgggacggct cccttaactc cgattgcgct tcattcgcag cgattccatg cggcatggct
     4321 ttctggaatt tgggtggcgg gctgcttgtc aggccattat ctggagtaaa atccgtcact
     4381 tcagccagca acttgggatc gggctgggac ccgggagcgc ctcgccgccg gcgcacggcc
     4441 gggtcaatcg gcagttcttg cggcacccgt aaatagcgta gcacttcttc tgccactcgc
     4501 gggaataccg gcgccgacac gctgccgccc atgtgcatcc cgcgaggcga atccaaaatg
     4561 atcgctacta caatggccgg attgttcacg ggcgcgatgc cgacgaagga cgccacgaaa
     4621 ttggtcttcg aataagcgcc ggtgcgagga tcgatcttct gcgcggtgcc cgtcttaccg
     4681 ccggcggtgt atccctcaag ccgcgccagc ttgccggtgc cctgaaggac caccatttcc
     4741 atcatgtgcc gcatttcggc ggcggtttcc tgagaaatca ccctccgtgc cggaggatgc
     4801 tgcatcttga ccggcggctt accgtcctca aacgtcgcct cgatgatgtg gggcggcgcc
     4861 agcacgccgc cattggcgat ggcgccgaca gcttgcacca actgaattgc tgtaacaccc
     4921 acctcctgtc ccattgagat ggcgccgatg gagatcttcg accattgctt gggaggcttg
     4981 gtcaagccgc gagcttcccc gggaagctcg attccggtgg gctggccaaa gccgaatctc
     5041 cgcatatatt cgtacatgcg gttgtcgccc agccgcaaac ccatcttgat cgccccgaca
     5101 tcactggagt tgacgaggat ctgttcgacg gtcaggtttc caaatggctt gtggtcgcgg
     5161 atgcgatggc ccgctacgac aatggatccc atctggcaat cgacgacttc agtggggcgc
     5221 gccacttttt cttccagggc tgccgaaagc gtaaccagtt tgaacgtcga tccgggttcg
     5281 aacgacgagc tcacggcgaa attgcgcctc ttctcaacat cggcgggtga gcgaaccggc
     5341 tggttcgggt tgaaggtcgg ataattggcg atggctagca gagcaccggt cgatggctcg
     5401 agcaccacga tgctgccggc ggcggcggcc gttcgttcga tcgccgcatt cagttcccgc
     5461 tcggcgacgt attgaatgcg ctcgtcaatg gtgagaatga cgttcgttcc ggggtcgggc
     5521 tgctggctga gccgcccgtc aaattgtttc tgccgcgcgt cggtcgagac cagcattaag
     5581 cccggccggc cctccagttc gctttcccgg gcctgctcaa tgccagccag accggtttga
     5641 tccaacccta cggcgccgat caagtgggcc gcggtggatc ctttcggata gtagcgcttg
     5701 ctttccttct ggaaatagat cccgcgcaag tcgagcgcgc gcacgcgatc agccagttcc
     5761 ggatccacca ttcttgcgat ccagcagaag ccatggccgg tggagagctt cttgatcagt
     5821 ggattgcggg ggatgccgag aattctcgcc aggattgtgc cgactccctc gggatcggaa
     5881 atttccgccg gcatggcgca gatggaatcc acgggcatgc tcatggcaag ttcgcgcccg
     5941 cggcggtcga gaatcaagcc gcggctcgcc cgcaccggga ttgcgcgctg ttgttgcagc
     6001 ctcgcccgct cttcaaaaaa gccgcgccga atgatttgga gatagacgag ccggctgaag
     6061 acaacggccg cccaggcgag cgccacgatg ctgaaccaca cgaccttccg ccggcgcatc
     6121 gccatcgccg gatcaaagat cggatccaaa ccgcctaact cgatggccgg cgggaagaga
     6181 ctgccctgta cgggtcccgg ggccggagag cgcccgccgc cagatcggct agaagttcgg
     6241 cgggccattc ctatctcgcg cgctgccgga tcgtcacagg cgaatttctg gccagcattt
     6301 cgcgcccggt gggaagattc ccgtcggcct cgggccagat cacctgttcg ggcacaggct
     6361 catcgagccc cagcttgcct ttcgcaatag tgtaaatccg ctgcgggtcc cgaagcgtcg
     6421 cctctttcac ttgtaactgg cggctcgtct ctaccaaggc ctcgtgctct ttcttcaaat
     6481 cctgcaagcg gtaacccgtt ctcaaaatgg ccagagaggg gacgatgtag agcagcgcca
     6541 aaaagaaaaa ggcggaaacg caaccgatca ggccgaggca ctcccggcgg gagttagggt
     6601 ccacgacgcg aaccagcctg gagttgtcga tggccttgac gaagaaacag aggtcttcca
     6661 tcggaagcgg ccgcgtggca gcgggacggc gagagagtcc cgcgcgcacc tcgcgccccg
     6721 ctgccactcc gtcgtaaaaa aatcctttgt aaggccggct gtcgtgtctc tccggcgctc
     6781 cagtttgggt ttccatctcg atgagcatct attcctcttc agccgtcgtt ctttccgccg
     6841 cccggagctt cgcgctgcgg ctggcggggt tagcgcgaac ctcttcttca ctgggcctga
     6901 ttacgtgctt ggtcaaaatc ctgagcaagt ggcgctgcgc ccaacgctgg aatccctgct
     6961 tgaccgcgcg atcctccaga gaatgaaagc tgatgatgac gaaacgaccg ccgggcttga
     7021 gcaacggcgg cgcggattcc aataaccttt ccaattctcc caattcatca ttcactgcaa
     7081 tccgcaaagc ttgaaacgtc cgggtggccg gatcgatccg tccacccccc gttcgcggcg
     7141 cggcacgctt gattacttcc gccagttgcc ttgtacctgt aagcggccgc gcccgaacga
     7201 tggctctggt tattctccgc gacctccttt cttctccata tctgtagatc agatcggaga
     7261 gcggcttttc gccgtattga ttcacgattc gttccgccgt cagcgtttga tcaggatcca
     7321 tacgcatgtc gagcggcccg tccgtcaaaa aactgaagcc tcgatccggc gcttcgagtt
     7381 gtcccctcga aactcccaag tcggccacaa tgccgtctac tgcttcgact cccacctgct
     7441 gcaagatttg cggcagttcg ctgaaccggg agcggcgcag catcacccgg tcgccaaaag
     7501 ccgccagccg ctggcgcgct gcttcgatcg ccctgggatc gcggtcgatg gcgatgagcc
     7561 ggcctcccct gagacggccg gcgatcgccg ccgcatatcc gccctcgccc acggtgcaat
     7621 ccacatagat cccatctggg gagaggcgca accactcgat cgcctcgttc aagagcaccg
     7681 gaatatgcgc catgcatcct gaaatccccg gccctaaacc cccagcttgg ccaagttgtc
     7741 gaagtcctca ggagtcaagc gattccgctc cgcctcctcc tcgtaccggg cttggctcag
     7801 cacctcgatg tgatttgtct cccagttcag cttcacttcc cccttcatgc ccgcttgatc
     7861 gcggagcttg gcggggatca gcagccgccc ctgttcatcc aggctcgctt ccgccccgta
     7921 ctggttggcc tggaacagga acttcttttt cagcttgtca aactgcggag cctgcgataa
     7981 agtttgctcg attcgctccc attcgctgat cggataaatg agcacaatgt ccccggtgat
     8041 gctggtcacg aaatatatcg aattcgcccc gtatcgctca tccagacgtt ccttgacgaa
     8101 ggcggggagc ttcagcctcc ctttgtcgtc cactttgacg gtcgccgtgc cgcggaacat
     8161 tcaggattgc tccaggttga gccactttta tccgaaatac gccaaaatct tccactggct
     8221 cccacataaa agtgccttaa aacaaagcct gtgtcaatag aaattttccg ctctaaattc
     8281 ttggcccgcc agggcctcgg cgaggcatac tacatgttgg ggcatttttt actggctttc
     8341 tatagatagg gcgggctagg aggcggttta cccgaggcgg tccaccctaa gtacggcgct
     8401 aactaggcga agaagactca gtctttatgg ggctcggtcc gataacccgt gcgcgttcga
     8461 acggagagct ttttgtgatc cggcgtttcc gccgtcacct tgatggagtg gtaagaccca
     8521 tcggcaacgc tgggcgcata tccgatcgtg tactggctgc ggatgtcgtg agcgatccgt
     8581 ttgcagatgc ccaccaagtc ccggtgctcc cttgaaagat aggcctcgcc gccggtcaag
     8641 tgcgcgagct ttttcagcac cttggggttt cgatcgggat cctgatcatc aaagagccca
     8701 atcgtataga tgatcacgtt cgattgctcg gcggcggcca ggacgtcggc gtagctggca
     8761 cggctggcat tatccccgcc gtcgctgaag acgacaagta ttttcttttg gcgtctgcct
     8821 cgctcgagtt gcttgagtcc cgtggcgatg gcgtcgtaca gggccgtcct gccggcggga
     8881 ggaaagcgca tcaaggcggt caacatctgg ctacgctcgt tggtaaaagg cgattcgggc
     8941 gggaggccca ggaaaacctt ctcgttgaaa ttcaccacga acatttcgtc gtccgggttg
     9001 ctatattgca gaaatcccag ggcggccatg atggtctccc ggcgtttggg catcatgctg
     9061 ccgctgccgt cgacaacgag gccgatggtg acgggcacat cctccggctc gaagagcttg
     9121 atctcttgcg gccggccgtt ctcgtagacc cgaaaacagt ctcgccccag gccggacacg
     9181 gatccacctc catgatcgcg gacggctacg ccgagcacga ccaggttgac gtccatggaa
     9241 attttgaagt cgcccgtggc gcgttctgcg gcgccggcgg gctgtccgct ggaagccgcc
     9301 tgcgggcggg cccaaagaaa gaggggaacc tccagcgcca gcaataagac gatcactgct
     9361 gcgtgttcgc gtgtagtcat cgggcgattt cttccccggc gccgccggag ttctggcccc
     9421 atagcagcgg gtaaggcccg ctcgagctaa agccgaaaac ggcgcctttc tgcgccgacg
     9481 caagcccggt catgagcagc tttgcagggg atggaagaag atgaagttcg agcgttgcgt
     9541 gcagcgttgt cagtctcagg cagcgccaac gaagttggcg ccggaaccgt gtggtcccgg
     9601 ttcagccctg gccgccaggg atgcgcatcg ccacgggaaa gcgcgccagg gaatccgcgc
     9661 cggtatgcac ccagcgaaca aacggtgcgg cgattcacga tccagctccg gaactattat
     9721 acggattgca gcacaaggga gcatagcccg aaaggcatgg tttttccaag cgtgcgtgct
     9781 ccataataca ttagcgtaga gcctatgccg catcgattga accgccggca atggatcatg
     9841 ggctgcgcca gtctggcgct gctccgcggc gccgagcgcc cgcgtctgaa gatcacggac
     9901 atggaagtat tcgtcgtcaa agtgaacgtc cgaggcaact ggatttttgt gcgcctcaag
     9961 accgacctgg ggctgaccgg gcttggcgaa gcttcccacg gcggcggctt ccagggagag
    10021 ggagacgctc aaatgccgac ccagttggcc gaatactttg ggctggtccg tggcggctcg
    10081 ccgtttgaaa tcgaggccta cagagcgcgg ggacgggcgc gtgctaaggc tggcggccgg
    10141 ctggcggcga ccgcattcag cgccatcgaa caggcgcagt gggacctggc tgcgaaagcg
    10201 ctgggagcgc cgctctatga gcttttcggc ggaaagctaa gaagcgaatt ggacgtttac
    10261 gcgaacatca accgggcgac caatgaggac cgctctccgg ctggcttcgc cgccaacgcc
    10321 agcaaagcag tggaagaagg ttttcgggcg atcaaggccg ccccgttcga cgatttccca
    10381 aagctggacg ctccggccgg cgagcggcag aagttcgccg agttaggcat agcgcgcgtg
    10441 gaagccatac gacgcgccgt tggcccgcac ccgaatctgc tgatcgattg ccacagccat
    10501 tttgatcccg cgcttggtat cgaagtcgcc cggcgcctgg aaccgcagaa tctctactgg
    10561 tatgaggaac cggttcctcc cgaaaggatt gaggagagcg cagcgattcg ctcgaaaatc
    10621 tctcagcgca tggcgggcgg ggaacttctt tttggcttgg aaggttttgc gcccgtgtgc
    10681 cgtcggcgat cgatggacat catcatgccc gatgtaaagc attgcggcgg gatcctcgag
    10741 gctttgaaga tttccgtgtt ggcggaagcc gatggcgtcc tagtggcgcc ccacaatccc
    10801 agcgggcctg ttgcgacggc cgccagcgtg cagctctgcg ccggattgcc caatttcctt
    10861 accctcgagt acgcttgggg tgaggcgccg tggcgtaagg atctgatcat tccgccggaa
    10921 gagttttcga acgggaagat ccgcgtctcc gaccggcccg gctttggcat cgaactcaac
    10981 gagaggctcg ccagggagca cgcgtaagcg cctcttgcgg tgagaagccg ggaatacggt
    11041 cgggcgcagg attgggcgac gttttcgctt ttcgccatgc gatccggcga tcgccgaagg
    11101 ccaagccgcc cgcatggatt tctattccag agccgaatta tgttcacaaa tgccagctca
    11161 cgttaatttc ttggctttcg acttgggagc agaaagcggc cgggccatgt tgggccgctt
    11221 tgatggcgcg cgcctcgctt tcgaagaagt gcaccgtttt cccaacagtc ccgtgcgggt
    11281 gctggacagc ctgcattggg atgtcttgcg cctgttcggc gagctcaagc gggggctggc
    11341 ggccggggca aagcaggcgg gaggcggcct ggcctccgcg ggcgtggata cttggggcgt
    11401 tgactacgct ctgctgggag ccgacggagc gctgctcgaa aatccgtatc actaccgcga
    11461 ttcccgcacc gacggcgtga tggaaaagct tctggccacg gtgccgcgag aagaggtttt
    11521 tgaacggacg ggcattcaat tcctgcgatt gaacactctg tatcagctct atgctctgaa
    11581 gctccagcgg ccgcatgtcc tcgagcggtc gcacacgctg ttgatgatgc ccgacttgtt
    11641 ccattattgg ctgacgggcc gggccgtgag cgagtacacc atcgccacga cgacacagtt
    11701 ttacgatccc cgatgcggtt tgtgggcgcg cgatctgctg gagaagctgc atctgcccgt
    11761 gcggatcctt cctggcattg tgccgcccgg cacgcagctc gggccgttgc ttccttcggt
    11821 ggctgccgag gccggcattc agcccatgcc ggtgattgtg ccggcctgcc acgatacggg
    11881 ctccgctgtc gctgccgttc cggctcagcc gggcggcgat tgggcgtttc tcagctctgg
    11941 gacgtggtcg ccgctgggcg tggaattgcc tgcgccgatc atcaatgggg cggcgctagc
    12001 cctgaatttc accaacgagg gcggcgcttg gaccaccact cgcctgttga agaatctgac
    12061 gggtatgtgg ttgttcgaag agtgccggcg ggaatgggct cgggaaggga tgccaacggg
    12121 ctaccaggga ttatgcgaag cagctttggc cgcggaccct tttcgctcga tcatcgatcc
    12181 cgacgacccg atgttcctga ctcccggcgg tatgccgggc aaaatcgtca ctttctgccg
    12241 ccggacgggc cagccggtcc ctgaagatca gggagctttc acgcgggcga tcttcgagag
    12301 cctggccctc tcctaccgct ctgcactgga tgagctcaga cagattctcg gccggaatct
    12361 gcggactttg cacatcgtag gcgggggctc gcgcaacccg ctgctttgcc ggttcacggc
    12421 cgatgccacg ggctgcgaag tccttgctgg ccctgaagaa gcgacggcga tcggcaatgt
    12481 acttcttcag gcgatggcct tggggtatgt gggcacgccc gtcgaggcgc gggatctcgt
    12541 gcggcgttct ttcccgccga agcgatacga tcccgcccgg cgggaacgct ggaacgaggc
    12601 tgcggcgcgc cgcagagacc tgattaccag ggctgcggcc gcttcgtaaa tttgcggccg
    12661 atctccgccg cttccggtcc cgaggccccg atcatgacgc cttcccggat catgtcgatg
    12721 acgtcgttgg gccgaacctg attcagaaag gcgagcttat tcgccttgca ggcggccaga
    12781 actttggctc gggctgcgat catctccggc ggatacggcg ggtcatggtt ctcaggaaat
    12841 ccgagcgaat agcccatgtc gcccggcccc cattcggcaa aagcgaggcc gggaaccttc
    12901 aggctctgat cgacgttggc taacgcccgc ttatcttcaa tcttaagccc gagcatgatc
    12961 tcgccgttgg gattcagcgg ccagacatcg gccttttgcg cgtattcctt cggcgaaatt
    13021 ccccagatcc gggcggcgct ggcggtgccg tgggcgccgc gccttccctc gccgagacct
    13081 tcgttcacgc ccaggtgctg gaacgggaac cgcgccgact ccacgaaagc ccgcaccgct
    13141 ccagggtcat ccgcgtggca aagcagaatc ccgtggatgc cggtcgcgag cacctgctta
    13201 accatccacg cgttggcgcg cattgtcgcc tcatctgtcc cgtcagtcgg cagcgtcaca
    13261 atcaccgcgg gagtgcgatg tccgctcttg gtggggcctc ccttcaccaa gccgcgcatg
    13321 aagtcggcga gcttggcgac atcgaacggg ctgtgctcca tatcgtaggt aatgtaatcg
    13381 gcccacgttt gggccatttt cacgccctct tcgtatccgc catgcccgct cgtgtagtaa
    13441 atcggctggc cttgcgagag cagatcgatt gctttgttga tatgtttcgg ctgttgcgcc
    13501 gcagcgctgc ctgccgtcag aatcaggacg agaaccgcgc cgagcgccgc atgaggatcg
    13561 cgtgctctct tcatggaaac ctccaactgt gcaagattat acccgcaccg cgcgcagcgc
    13621 ggcgcacgaa agcatcggcc caagggcgct gcagccggtt aggtacaatg aaaaaaaagg
    13681 gtaaagggag aagtggacca gccagtaccg atcgtggaac atccgggaat tcggagagcc
    13741 ttgctgcatg tgctggtcta tgcggccgcg gcggccagtc tggcatgggt tctttacgat
    13801 tttcaatggg gagactttct cgagaatctg aagtttctgg actggcgctg ggtggttccc
    13861 g
//
LOCUS       DASREK010000244         9897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1275879, whole genome shotgun sequence.
ACCESSION   DASREK010000244 DASREK010000000
VERSION     DASREK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275879"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFA54_12960"
     CDS             <1..412
                     /locus_tag="VFA54_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51769.1"
                     /translation="EVGAKVALKDCVPPAFTLNDAGLTANKPLLLDTVPVNASVEPLV
                     KATDWGAEVVPVDTLPKFSEAGLRDKLSAMPVPVTATVRVRGQDGFWGSVAVMLIVPR
                     AAPAAAGANEIWTLPEGETGDTVKAPELLVAENW"
     gene            593..859
                     /locus_tag="VFA54_12965"
     CDS             593..859
                     /locus_tag="VFA54_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51770.1"
                     /translation="MNPAAVPAAVGAKVALTFVAPGALKVDGLTVKAPLVKLRLKVRG
                     VAGKIVNLWEEEHPRRTFPKLNELGEKVNARSPVPLTATFCDVV"
     gene            878..1084
                     /locus_tag="VFA54_12970"
     CDS             878..1084
                     /locus_tag="VFA54_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51771.1"
                     /translation="MIVSVPLKLAAEVGVNVTDTVPCCWSLMLDGLMPAENAGLSDTT
                     LKLVGKSLGVVLVTVNVIVALAPR"
     gene            complement(1200..2225)
                     /locus_tag="VFA54_12975"
     CDS             complement(1200..2225)
                     /locus_tag="VFA54_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51772.1"
                     /translation="MRYGTFAIVSILFCGSLISARADGLISGPTLGYLWDQQYAGLRP
                     ILGIPGASVVGPVMNLGGAFRSVEVSPGLEYALAIAAGSGKVLLVDLRTGAPAGREID
                     GALINPARIIFSPLGRFAGLYDSQLRSVQVIGGLPDAPAIAGTADLSGPPSVLTAMAI
                     SDQDGLVLAAASENRRGSVFAVSPGNPPRFVAQVGRASALSFLEGSQDALIADYDRSE
                     LLLVRDVQGAAQALTLAGPADGIANPIAIASAGRDGRVLAISESQITVLDLNGSAPAA
                     LPCACSLSGIHRLSGNGVFRLDSAAGKTLLLDAGASNPRILFVPSADSASVSPPRLPG
                     FSGRIRR"
     gene            complement(2238..2879)
                     /locus_tag="VFA54_12980"
     CDS             complement(2238..2879)
                     /locus_tag="VFA54_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51773.1"
                     /translation="MSSGPGGFRGGRALRAGISIGLACLIATQAARPAAGAQAEGAPG
                     LQIVILDGEGAINNIRQRTAREPIVQVQDENHRPVAGAVVVFSLPERGAGGAFANGST
                     TMTVTTDAQGRAVATGLRPNSVQGNFQIHVSASNQGRTATAVINQTNSLSAAAAAGGI
                     SAKTLAIILAVGGGAAVGGIVAATRGGRSAPTAAPGPPATTITAGTPTVSAPR"
     gene            complement(2987..4237)
                     /gene="glgC"
                     /locus_tag="VFA54_12985"
     CDS             complement(2987..4237)
                     /gene="glgC"
                     /locus_tag="VFA54_12985"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HZS51774.1"
                     /translation="MKLRVLGVVLAGGKGSRLYPLTKERAKPAVPFGGKYRIIDFVLS
                     NLINSGIYSIYVLTQFKSQSLLQHLSDGWQFANLLKNQFIIPVPAQMRKGEIWYQGTS
                     DAIFQNINLIEQADPHLVAVFSADHVYRMDVSQMIAYHEEKRAAGTVAALPMPRERAR
                     EFGTMEITPDWRIVSFHEKVPDPPEIPGRPGWTLASMGNYIFETRILVRELYDDAGNP
                     ASNHDFGRNILPDMIHRYPVFAYDFLTNEIAGEPPQNQGYWRDVGTIDSYYDANMDLR
                     SVSPSLNLFNRQWPLRTASYQEPPAKFTFDEEGRRGHALDSIVSEGCIISGGTVRGSV
                     LGRRVFVHSYSLVEDSIIMDNCDIGRGARIRRAILDKNVRVPAGTEIGYDLDRDKKYH
                     HVTESGIVVVAGQRSPVEIASIVI"
     gene            4566..5738
                     /gene="aroC"
                     /locus_tag="VFA54_12990"
     CDS             4566..5738
                     /gene="aroC"
                     /locus_tag="VFA54_12990"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZS51775.1"
                     /translation="MLRFETAGESHGECLVATVTGLPAGVPVSLAFINRQLWRRQQGY
                     GRGGRMKIETDCAEIVSGVRHSKTIGSPVALLIRNRDWENWRESLPVEDYPGSAEKAK
                     PVTRPRPGHADLAGCLKYDFPEARYILERASARETAARVAAGAVAKLLLSEFGIEVLA
                     HVIAVGHAALGRPAAWEEIAALEKRSEILLGCVDSAVEQRMKDVVDEAYRTGDTVGGV
                     FEVVAHNVPPGLGAHTEWDRRLDARLAQAIVSMQAVKAVAIGEGVENAARFGSEAQDP
                     IRYDPQQRRFLRPSNRAGGIEGGMTNGEDVVVRGYLKPISTLRKPLESVDLRTRATSL
                     AAYERSDVCVLPAAAVIGEAMVALALAGALIEKFGGDSLPEMKRNFDGYLEQVKNF"
     gene            5923..6438
                     /gene="def"
                     /locus_tag="VFA54_12995"
     CDS             5923..6438
                     /gene="def"
                     /locus_tag="VFA54_12995"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZS51776.1"
                     /translation="MIYKIVKYGDPVLEERAKPVTEFDTPELQELVRDMFESMYAAKG
                     VGLAAPQIGISKRIAVIDVSLGENEKDKLVLINPEIIHTEGRQNGEEGCLSIPGFRED
                     VRRPRRVTVRAQDIHGESFEITGEELLARAFLHETDHLNGRLYISHISPLKRDLIKRK
                     VRKLIRQGEWT"
     gene            6442..7404
                     /gene="fmt"
                     /locus_tag="VFA54_13000"
     CDS             6442..7404
                     /gene="fmt"
                     /locus_tag="VFA54_13000"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZS51777.1"
                     /translation="MELSGLKAVFMGTPQFAVPSLQALADAGVAVMEVITQPDRPAGR
                     GHRPAISPVKALALKLGLPVFQPLKIKDPEAIGRIGRAEPDVIVVVGYGQIIPRALFD
                     LPPLAAINVHASLLPKYRGAAPIQWAIARGETTTGVSTMRIEARLDAGDIFLQRETAI
                     GAEETAPELSARLAILGADLLVETLRGLRSGQISGKKQNDAEATYAPLLKKEDGRIDW
                     RSSAREIFNRVRGFDPWPGAYTTFRGGLLHIRRARLLGDIAEPGNTPPGALVVDSGRL
                     RAAAGQGWLELLEVQPEGKKKLLAQEFINGWQPKTGEILGDKNP"
     gene            7401..8579
                     /gene="argJ"
                     /locus_tag="VFA54_13005"
     CDS             7401..8579
                     /gene="argJ"
                     /locus_tag="VFA54_13005"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZS51778.1"
                     /translation="MTLPLGYRFAATYAGIRKISKPDLALMVSDRPAAAAAVFTTNLV
                     KAAPVILSVRHLTRSKGYARAIVANAGNANCATPAGLAVARATAKSAAAALGSLEHEV
                     LIASTGVIGVPLDEKRITAALPGLASSLSPENFEAAALAILTTDTVPKTAWAEVKLKT
                     GLVRVAGMVKGAGMIHPRMATMLGFLFTDAAARPAELQSLLASVTEATFNRISVDGDT
                     STNDTVFLLANGASGVRPSGRERQRLQAAIAEVAEKLAIAIVRDGEGAQKLLTIDVEG
                     AANDRAAATIARAIANSPLVKTALAGADPNWGRILSAAGNSGAKFDPAKVDIDLNGFA
                     VCRRGTAAEFSEDEVKRSLAGRESSLRFLIRGQGKGRARFWTCDLTEEYVRINANYRT
                     "
     gene            8560..8985
                     /locus_tag="VFA54_13010"
     CDS             8560..8985
                     /locus_tag="VFA54_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51779.1"
                     /translation="MPITARSLICALCFGAILAAGPPEADEVSSLLLDLSTSLQEGSA
                     ARFLSHIDHRFKDYAVLEDNVVAMTAQDEVASSVAVLEQRKTGEGYELRLDWLLELKT
                     ISGDKPAERRRSEVICRIKRSGKQWKISALAPVTFFRPL"
     gene            9150..>9897
                     /locus_tag="VFA54_13015"
     CDS             9150..>9897
                     /locus_tag="VFA54_13015"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HZS51780.1"
                     /translation="MTFPAQFYIRNVPIRPATVLAPMAGVTDTVFRRFIRGLGSCGLL
                     MTEFTSSNGISAALHSPGQNKTLRYLHFEPDERPITAQLFGADPQVMADAARVVEDLG
                     FDIVDLNFGCPVKKVVRCNGGSGLLRDLPLVERILRAVRSAVKMPFTIKFRTGWNRDE
                     IVDTRLARLAEDCGVEALALHPRTREQGYSGHSDWSRIAAVKACVKIPVIGNGDIQTP
                     EDAVRMVAETGCDAVMIGRTASSNPWIFRQI"
BASE COUNT         1996 a         2997 c         3114 g         1790 t
ORIGIN      
        1 ggaagtaggg gcaaaagtgg cgctaaagga ttgcgtgccg ccggcgttca cactgaacga
       61 tgccgggctg accgcaaaca aaccgttgct gctcgacacc gtccccgtga atgcgagcgt
      121 cgagccgttg gttaaggcca cggattgggg tgctgaggta gttccggtcg acacattgcc
      181 gaaattcagt gaggcgggac tgagagataa attgagcgcg atgcccgtac cagtgaccgc
      241 gacggttcgc gtaagagggc aggacggatt ttggggatcg gtcgcggtaa tgctgattgt
      301 cccccgggct gccccagccg cagccggcgc gaatgaaatt tggacgctgc cggagggcga
      361 aaccggagac accgtgaaag cgcccgaact gctggtagcg gaaaactggt agacggtctg
      421 cacagaagga ttagccgata tggtcacggt tctgggagac gatgtcgagc caaccggcac
      481 ctgtccaaaa tcgagcgccg ccggcagagc cgtgatgtcc actccggtcc ctacaatggg
      541 aagcagttgc ggttgcaccg actgtccctg ctgcgttact gcgtttaagt ttatgaaccc
      601 agccgccgtg cccgccgccg tcggcgcgaa agtcgctttg acattcgtcg cgcccggagc
      661 gttgaaggtc gatggattga ctgtgaaagc cccgctggtc aagctcaggt taaaagtcag
      721 aggcgtggct gggaaaatcg taaatctctg ggaggaagaa catccaagaa ggacattccc
      781 caaactcaac gaactcggcg agaaggtaaa tgcaagaagt ccggttccgc tgacagccac
      841 gttttgtgat gttgtctgaa aacccgtctg tgaagcagtg atcgtaagcg tgccgctgaa
      901 gctggcggcc gaagttggtg taaacgtgac cgacacggtt ccctgctgct ggtcgttaat
      961 gctcgacggg ctgatgcctg cggaaaacgc gggattatcg gatacgacgc tgaagttagt
     1021 tggaaaatcg ctgggcgtag tattggtcac ggtaaacgtc atagtcgcgc tggcgcccag
     1081 gtaaacattc ccgaaggcga ccgatccggt ggatacgcta aaactcgacg gcggcggtgc
     1141 agtcgccgcc cccgctccgg caacgaataa cgagaatccg acaagcagcc tcccaagcct
     1201 catcgccgga tcctccccga gaagccgggc aagcgcggcg gcgaaaccga agcggaatcg
     1261 gccgagggaa caaagagaat ccgcggattt gaagccccgg cgtccagcaa cagagttttg
     1321 cccgcggccg aatccaagcg gaacaccccg ttgccactca agcggtgaat tcctgataga
     1381 ctgcacgcgc aaggaagcgc cgccggcgcg ctcccgttca aatcgagcac ggtgatctgc
     1441 gactcggata ttgccagcac gcggccatcg cggccagcag aagctatcgc aattgggttc
     1501 gcaatgccgt ctgccggacc agccagagtc agagcttgcg cggcgccctg cacgtcgcga
     1561 accagaagca actcgctccg atcatagtcg gcaatcaagg cgtcctggga cccctctaga
     1621 aaactcagag cgctcgcccg cccgacctga gcaacaaagc gcggcgggtt tccgggactc
     1681 acggcgaaaa cagatccgcg gcggttctca gaagcggcag cgagcaccag gccgtcttga
     1741 tcgctgatgg ccatcgccgt cagtacagaa ggcgggccgc tgagatcagc cgtgccggca
     1801 atcgcggggg cgtcgggtag accgccgatg acctgaacgg atcgcagttg cgagtcatac
     1861 aacccggcga acctgcccaa cgggctgaaa atgattcggg ccgggtttat gagggccccg
     1921 tcgatttccc gccccgcagg cgcgccggtc cgcaggtcta ccagaagcac ttttccggag
     1981 ccggcagcga ttgccagagc atattccagt ccgggggaaa cctcaaccga acgaaacgca
     2041 ccgcccaggt tcatgaccgg ccctacaacc gacgcgccgg gtatccccag gatgggccgc
     2101 agcccggcgt attgttgatc ccataggtag ccgagcgtcg gcccgctgat cagtccatcc
     2161 gcccgcgcgc tgattaggga gccacaaaac aaaatagaaa cgatcgcgaa agttccgtat
     2221 ctcatgtcca cccgccgcta gcgtggcgct gaaacggtcg gcgtgcccgc cgtgatcgtg
     2281 gtggctgggg gacctggcgc cgccgtgggg gcgctcctgc cgccccgagt cgccgccaca
     2341 atcccgccga ctgctgctcc gcctcctaca gccagaatga tcgccagcgt cttggccgag
     2401 attcccccag cggcggctgc cgccgagagg ctgtttgtct ggttgatgac ggccgtagcg
     2461 gtcctgccct ggttggatgc gcttacgtga atctggaaat tgccctgaac gctgttaggc
     2521 cgcaaacctg tagccaccgc gcggccctgc gcgtccgtgg tcaccgtcat ggtcgtgctg
     2581 ccgttggcga aggcgccgcc cgcgcctcgt tccggaagcg aaaacaccac cacggctccc
     2641 gcgaccggcc ggtgattttc atcctgcacc tggacgattg gctcgcgggc cgtgcgctgg
     2701 cggatattgt ttatggcgcc ttccccatcc agaatcacga tttggagtcc cggcgcgcct
     2761 tcggcctgag ctcccgctgc cggccgcgcc gcctgggtcg caatgagaca agccaagccg
     2821 atcgagatgc ccgcccgcaa agctcgaccg ccccgaaagc cgccaggacc ggatgacatc
     2881 tgtgcgcctc ctcacacctc cgcccccgta ttatgcccga atgctcaaaa aaagaacagt
     2941 ctcaattgcg ggaacgcgac ccccgaaagg gcggcgaaag gttttcctaa atcacgatgg
     3001 atgcgatttc gaccggggac cgctggcccg cgaccacgac gatgccgctt tccgtcacat
     3061 ggtgatattt cttgtcccga tcgaggtcgt agccaatttc ggttccagcc gggacgcgga
     3121 cgtttttgtc caatatggcc ctgcggatgc gcgcgccgcg gccaatgtcg cagttatcca
     3181 taatgatcga gtcttcaaca aggctgtaag agtgcacaaa gactctgcgt cccaaaaccg
     3241 acccccgaac cgtgccgccc gaaatgatgc acccttccga gacaatcgag tccagcgcgt
     3301 gcccgcggcg cccctcttca tcaaaggtga atttggccgg aggctcctgg tagctggccg
     3361 tccgcagcgg ccactggcgg ttaaaaagat tcagcgaggg cgaaaccgag cgcagatcca
     3421 tgttggcgtc gtaataggaa tctatagttc ctacatcgcg ccaatatcct tgattctgag
     3481 ggggctctcc ggcgatctcg ttcgtcagaa aatcgtaggc gaagaccggg tagcggtgaa
     3541 tcatatccgg aagaatattg cggccaaagt cgtgattgct cgctggattg ccggcgtcat
     3601 cgtaaagctc gcggaccagg atgcgcgttt cgaagatgta gttgcccata gaagccagcg
     3661 tccagccggg ccggccggga atttccggcg ggtccggaac cttttcgtgg aaggacacga
     3721 tccgccagtc cggggtgatc tccatggtgc cgaattcccg cgcccgttcg cggggcatcg
     3781 gcagcgcggc gacggtgccg gcagcccgct tttcttcgtg atacgcgatc atctggctga
     3841 catccatccg gtacacgtga tccgcgctga aaaccgccac gaggtgcgga tcggcctgct
     3901 cgataaggtt gatgttttgg aagatggcgt ctgaggtgcc ctggtaccag atctcgcctt
     3961 tgcgcatctg ggccggcacc gggatgatga attgattctt aagcaggttc gcaaactgcc
     4021 agccgtcgct caagtgctgc aataacgact ggcttttgaa ctgggtcagc acatagatgg
     4081 agtaaattcc cgaattgatg agattgctga gcacgaagtc gataatccgg tacttgccgc
     4141 cgaacggaac cgccggcttg gcgcgttcct tcgtgagcgg atagaggcgc gatcctttgc
     4201 cccctgccag cacgacgccc agaacccgga gtttcatagc ccccaaatta accacattcc
     4261 ggacgggagg atcaatgctg acccctgccc ttgcccggcc tgcggcccgc aggcgggaga
     4321 acagaacagc ctttgcgcga ggaagccgga aggcaagggt cgcccccgtc cggcctgcgt
     4381 tttttgtctt gaaccagggg gcgcaagggc cgcttgtgac atcaaatttt tcccggagcg
     4441 ccgcctcagg tcctgcggcc gggcgcacca tcggcaaaac ctttccgccg ccatcttacg
     4501 ctggcgattg gatttccgac gatcgcttcc gctatcataa aacttctagc ctcgcctcgg
     4561 cgcctatgct gcgctttgaa accgcgggag aatctcacgg ggagtgtctg gtcgcaaccg
     4621 tgacaggact gcccgccggt gtgcctgttt cgctcgcgtt catcaaccgg caattgtggc
     4681 gccgccagca agggtacggc cgcggcgggc gaatgaaaat tgaaacagac tgcgccgaaa
     4741 ttgtttccgg cgtgcggcac tcgaagacca tcggctcgcc ggtggccctc ctcatccgca
     4801 accgcgactg ggagaactgg cgggagagcc tgccggtcga ggattacccg ggcagtgcgg
     4861 agaaagccaa gccggtaacg cggccgcggc cgggccacgc cgatctggcg ggctgcttga
     4921 agtatgattt tccggaagcc cgctacattc tcgagcgcgc gagcgcccgg gagacggcgg
     4981 ctcgcgtcgc cgccggcgcg gtcgcgaaac tgctgctttc cgaatttggc atcgaggttc
     5041 tggcgcacgt gatcgcggtc gggcacgcag cgctgggacg cccggcggcc tgggaagaga
     5101 tcgcggctct cgaaaaacga agcgagattc tgctgggctg cgtggattcg gccgtggaac
     5161 agcgcatgaa agacgtggtg gacgaggcgt atcgaaccgg cgatacagtg ggcggcgtat
     5221 tcgaggtggt cgcccacaat gttccgccgg gcctgggcgc tcacacggaa tgggaccggc
     5281 ggctggatgc aaggctggcg caggcgatcg tctcgatgca ggcggtcaaa gctgtcgcca
     5341 tcggcgaagg cgtggaaaac gccgcccgct tcggatccga ggcgcaggat ccgatccgct
     5401 acgaccccca gcagcgccgc tttctgcggc cgtccaaccg cgccggcggg atcgaagggg
     5461 gaatgaccaa cggcgaggat gtcgtggtgc gcggatatct gaagccgatc tcgacgctgc
     5521 gcaagccgct tgaatcggta gaccttcgaa cgcgcgcaac atccctggct gcttacgagc
     5581 gttcggatgt atgcgtgctg ccggcggcgg ccgtgattgg tgaagctatg gttgcgctcg
     5641 ccttggcggg cgccctgatc gagaagttcg gtggagattc actccccgag atgaaaagaa
     5701 atttcgacgg ctacttggaa caagtaaaaa atttttgagc cttgcggcgg ggcggccaaa
     5761 gaccgggtct ggggtctcgc cggaaaaagc tcccggggaa gaggtctatc ggcctgacac
     5821 agccgggcat tacatccggg cctccgaacg cctgaatcaa agatccggca cgaagcccgg
     5881 tcgccggcgc gtccgcaagg cgcaagccgg aatctgcatt cgatgattta taagattgtg
     5941 aaatacggcg atccggtgct tgaggagcgc gccaaacccg tcacggagtt tgacacgccc
     6001 gaactccagg agctggttcg ggacatgttt gagtccatgt acgccgccaa aggggtgggg
     6061 ctggccgctc cgcagatcgg catttccaag cgcattgcag tgatcgatgt ctcactcggc
     6121 gagaacgaaa aagacaagct cgtactcatc aacccggaga ttatccacac cgaaggccgg
     6181 caaaacggag aagaaggctg cctgagcatt ccgggattcc gggaagatgt gcggcggccc
     6241 cggcgggtga ccgtgcgcgc ccaggatatc catggcgaat cgttcgaaat aacgggcgaa
     6301 gagctcctgg cgcgggcgtt tctgcacgaa accgatcacc tcaacggccg gctctatatc
     6361 agccatatca gcccgctcaa gcgtgatctg atcaagcgca aggtgcgcaa gctgatcagg
     6421 caaggcgaat ggacctgagt tgtggaacta agcgggctca aagcggtatt catgggaacc
     6481 ccccaattcg ccgtgccttc cttgcaggcg ctggcggatg cgggcgtggc ggtgatggaa
     6541 gtgatcaccc agccggatcg ccccgcaggc cgcggccatc ggccggcgat ttctcccgtt
     6601 aaggcgctcg ccttgaagct ggggcttcct gtgtttcagc ccctcaagat aaaagatccg
     6661 gaagccatcg ggcgaatcgg gcgggcggag cccgacgtga tcgtcgtggt cggctacggc
     6721 cagatcattc cccgggcgct tttcgacctg cctccccttg ccgccattaa tgtacatgcc
     6781 tccctgcttc ccaaataccg cggcgcggcg ccgatccagt gggcgatcgc ccgcggggag
     6841 acaacgaccg gcgtcagcac gatgcggatc gaggccaggc tcgatgcagg agatattttc
     6901 ttgcagcggg aaacggccat cggcgccgaa gaaacggccc ctgagttgtc ggcccggctg
     6961 gcgatcctgg gggccgattt gctggtggaa acactgcgcg ggctgcggag cggccaaatc
     7021 tccggcaaga aacagaacga cgccgaggcg acctatgccc cgttgctgaa aaaagaggac
     7081 ggccgcatcg actggcgcag cagtgcaagg gaaattttta accgcgtgcg gggcttcgac
     7141 ccttggcccg gcgcgtacac aaccttccgc ggcgggctcc tgcacatccg ccgagccagg
     7201 ctgctgggag acatagcaga gccggggaac acgcccccgg gcgctctggt ggtggattcc
     7261 ggccgcttgc gcgccgccgc cggtcaaggc tggctcgagt tgctggaggt gcaacccgaa
     7321 ggcaagaaga agctgctagc ccaggagttc atcaacggct ggcagccgaa aactggagaa
     7381 attctgggag ataagaatcc gtgacgttgc cccttggata ccgctttgcg gcgacctacg
     7441 caggcattcg aaaaatcagt aaaccggacc tggcgctcat ggtttccgac cggcccgcag
     7501 ccgcggccgc agttttcacg accaacctcg tcaaggcggc gccggtgatc ctctcggtcc
     7561 gtcacctcac aagaagcaaa ggttacgccc gagccattgt ggccaacgcc ggcaacgcca
     7621 attgcgctac tccggccggc ctcgctgtgg cgcgcgccac agccaaatca gcggcggctg
     7681 cgctgggctc tttggagcac gaggtcctga ttgcttcgac tggggtgatc ggggttcccc
     7741 tggacgaaaa gcggatcacc gccgccttgc cggggctggc ctcgtctctt tctcccgaaa
     7801 attttgaggc cgccgctctg gccatcctga ccacagacac tgttcccaaa accgcctggg
     7861 ccgaagtcaa gctgaaaacg ggcctcgtac gggtggcggg catggtaaag ggcgctggaa
     7921 tgatccatcc gcggatggcc accatgctcg gcttcctttt tactgacgca gcggcgaggc
     7981 ctgcggagct tcaatccttg ctggcctcag tgaccgaggc cacattcaat cggatctccg
     8041 tcgatggcga cacctcgacc aacgatacgg tgtttttgct ggcgaatgga gcgtccggag
     8101 tgcggccgtc gggcagggag cggcagcgct tgcaggcggc gatcgccgag gtggctgaga
     8161 aactcgcaat cgccatcgtg cgggacggcg aaggtgcgca aaagctgctg acgatcgacg
     8221 tggaaggcgc tgccaacgat cgcgccgccg ctacgatcgc ccgcgccatc gccaactcgc
     8281 ctctggtcaa gaccgcgctg gccggcgccg atccgaattg ggggcgcatc ctttccgccg
     8341 cgggaaactc tggggcgaaa ttcgatccgg ccaaagtgga catcgatctc aatggcttcg
     8401 cggtttgccg ccgcggcaca gcggccgagt tttccgaaga tgaagtgaaa cgttcgctcg
     8461 caggccggga atcttcgctc cgctttctga ttcgcggcca gggcaaggga cgcgcgcgct
     8521 tctggacttg cgatttgacc gaagaatacg tccgcatcaa tgccaattac cgcacgtagc
     8581 ctgatttgcg cgctgtgctt cggggcgatt ctcgcggctg gccctccgga ggcggatgaa
     8641 gtctcatcgc tgctgctgga tctcagcaca tctttacaag agggcagcgc cgcgcgattt
     8701 ctgagtcaca tcgaccaccg gttcaaagat tacgcagtgt tggaagacaa cgttgtggcc
     8761 atgacggcgc aggacgaggt ggcgtcgtcg gtggcagtgc tcgagcaacg taaaaccggc
     8821 gaaggatatg aactcaggct ggactggctg ctcgaactga aaaccataag cggcgacaag
     8881 cccgcggaaa gacgccgcag cgaggtgatc tgccggatca aacgcagcgg gaagcaatgg
     8941 aagatctcag cgctggcgcc agtaactttt ttccgtccgc tataaagagc gcacgttcgt
     9001 acggccgctt gcccgcaaaa gatcctcgtt gtggcaagca gcgcgcacga atccataaag
     9061 accgtgctcg acgcgaagcc cctgtgccgc acggcgcagc ggctctccag cctccgaggc
     9121 aataagtggg tttgttaaac ttgacgagaa tgacctttcc tgcccagttc tatatccgca
     9181 atgtgccgat tcgcccggcc acggttcttg cgcccatggc cggcgtcacc gataccgtgt
     9241 tccgccgctt tattcgcggg ctcggaagct gcggcctgct catgacagag ttcaccagct
     9301 caaacggcat cagcgcggcg ctacacagcc ccgggcaaaa caagacgctg cgctacctcc
     9361 atttcgagcc ggatgagcgg ccgattacgg ctcaactgtt cggggcggat ccgcaggtca
     9421 tggctgacgc agcccgcgtg gtggaagatc tcggcttcga tatcgtcgat ttgaattttg
     9481 gttgtccggt gaaaaaggtg gttcgctgca acggtgggtc cggcttgctc cgcgatctgc
     9541 cgctggtgga gaggattctg cgggcagtgc ggtcagccgt gaaaatgcct ttcacaatca
     9601 aatttcgaac cggctggaat cgggacgaga tcgtggacac ccgcctggcg cgcctggctg
     9661 aggattgcgg cgtcgaggct ttggctctgc acccccgcac ccgggaacag ggttattcgg
     9721 gccattcaga ttggagccgc attgccgccg tcaaggcgtg cgtaaaaatc cccgtgattg
     9781 gcaatggcga catacagacg cccgaggacg cagtgcgcat ggtggccgag accggctgcg
     9841 atgccgtcat gatcggccgg acggcttctt ccaacccctg gatcttccgg caaatcg
//
LOCUS       DASREK010000245         9944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1105599, whole genome shotgun sequence.
ACCESSION   DASREK010000245 DASREK010000000
VERSION     DASREK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105599"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VFA54_13020"
     CDS             complement(<1..850)
                     /locus_tag="VFA54_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51781.1"
                     /translation="MNVSAGYTALHESAAWFDLSARGKLRFTGEDRVRLLHAMLSNDV
                     RSLQPGQGNYNFLLNAQGHILADANLYVFPDSILLDVEPEVTERVLQHLDKHIIADDV
                     QVENVTEKFACLALEGPASERMAGISASLEPGAHIESGGAIVARVSATGGPGFRLFVP
                     AEEKERLAARLEQAGAVAASVEDVRVVRVENGVPLYGEDFKETALPQETRQERALNFQ
                     KGCYLGQEIVERIRARGHVNKLLVRLGLDVDQPPGKGSAVIAGGQEVGRLTSPVYSPR
                     YQQSLGF"
     gene            complement(853..1284)
                     /locus_tag="VFA54_13025"
     CDS             complement(853..1284)
                     /locus_tag="VFA54_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51782.1"
                     /translation="MNWLDTLMQWLHLSAAVTGVGGLIYGRLVVGPSLAGLPQETRSK
                     LVGDLTTRMRPISFTVIAVLLVTGFYNYLTRLSERPPSYHMILGIKILLALHVFSVAV
                     LLAVPPGANPTRDARRPRLMAGAAISGILVLLLSAYLRRNY"
     gene            1583..2215
                     /locus_tag="VFA54_13030"
     CDS             1583..2215
                     /locus_tag="VFA54_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS51783.1"
                     /translation="MIHTILPVGILQCNCSIFGDETTREALVVDPGDELPEILKIVGQ
                     HGLHVTAIAITHAHIDHIGGAAALRRLTGAAVYMNERDLPIAKQLAMQAAWLRVPVPE
                     QPQIDVTVKEGDLIRLAGCTFQVLDTPGHTPGSISLLAAQENKLLAGDTLFRDSIGRT
                     DLPGGDSRHILASIKNKILTLDDSLIVVPGHGELTTVGREREHNYFLKGL"
     gene            complement(2229..2789)
                     /locus_tag="VFA54_13035"
     CDS             complement(2229..2789)
                     /locus_tag="VFA54_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZS51784.1"
                     /translation="MAAHLELLRPGTTALFIIDLQEKLFPAIWDKERLLVNVTKLLHL
                     ANILKLKTLPTTQYRQGLGATIPEVASLLPEAPMDKTSFGCLGDERIVKRLEQEVPRT
                     HTLLIAGIETHICVMQTVLGALAAGYRVHVAADATSSRSASNVQLGLERMREAGAVIS
                     STEMAIYELLARSDREEFKQMLPYLK"
     gene            2974..3858
                     /gene="pdxS"
                     /locus_tag="VFA54_13040"
     CDS             2974..3858
                     /gene="pdxS"
                     /locus_tag="VFA54_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZS51785.1"
                     /translation="MYLDESFRLKVGLAEMLKGGVIMDVTNAEQAEIAERAGAAAVMA
                     LERVPADIRKEGGVARMASIAKIREIMERVSIPVMAKARIGHFMEARILEALKVDYID
                     ESEVLTPADEEHHIDKRSFKVPFVCGARNLGEALRRIAEGAAMIRTKGEAGSGNIVEA
                     VRHMRTIQKEMKQLTVLGKEELVAEAKKLQAPLELVEYCAEHGKLPVPNFSAGGIATP
                     ADAALVMQLGAEAVFVGSGIFKSEDPERRARAIVKATTHYNDPQKLLEASEELGEAMR
                     GLDVAKLEASELLQTRGW"
     gene            3913..4527
                     /gene="pdxT"
                     /locus_tag="VFA54_13045"
     CDS             3913..4527
                     /gene="pdxT"
                     /locus_tag="VFA54_13045"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZS51786.1"
                     /translation="MKIGILAVQGDFEAHGRAVARLGATPVFVRAAGQLEGLEGLIIP
                     GGESTTMLKVLEEEGLFDAIRSFAARRPVLGTCAGAILMATEVSHPAQRSLGLIDMAI
                     ERNAYGRQVDSHISKIPPTAEFSQRTGGREPEEPLEAVFIRAPIIRRVGPGGIVLLRH
                     QNDPILVEQGSHLAATFHPELTSDTRVHGLFLAKAAASEPVSKA"
     gene            4551..4952
                     /locus_tag="VFA54_13050"
     CDS             4551..4952
                     /locus_tag="VFA54_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51787.1"
                     /translation="MRTLFVCIGNSCRSPMAEALARHLGADVIEPSSAGIYPASIIQP
                     ETLQVLEERGIKLEPRKPRSLFLLDAKQVDLLVNMSGAPLDNLLHSFLGRQIVWPVRD
                     PVGQPLGVYRAVRDEIEKKVSELIKELRQSG"
     gene            5385..5663
                     /locus_tag="VFA54_13055"
     CDS             5385..5663
                     /locus_tag="VFA54_13055"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS51788.1"
                     /translation="MTFNLSFRRQSFVVAVILFSTLAPVASRQARGQILQGTIDGNVT
                     DASQAAIVGARVVATEQRTNFTRDTTTNAGGLYTIPTLPPRCAREFLW"
     gene            5657..6667
                     /locus_tag="VFA54_13060"
     CDS             5657..6667
                     /locus_tag="VFA54_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51789.1"
                     /translation="MVTPPDVSAGTLPVPGTAALNTYDNANYVRGYIQTWNFTVEQRV
                     KTWIASAGYVGTRAVDPQNNIQMNWSPIGGGTAGEILNQLTGRTASTQLLGTLGSNTY
                     DALQTRAQRRFSGGYQVQLAYTFGKALGYAATPAVSIPQYYRLNRGPQSLDVTQNFEA
                     TGIVELPFGRGKRWAQSGIAAKTAGGWQLSTVFSAYSGRPFTATASTATLNSPNSSQF
                     ADCISAPHQTGNIFQWYDKSAFAVPASGRFGTCGTGSLRGPGLVNVDLGLDRKFPIRE
                     RFELRFRAEMFNAANTPHHVMPGGNASVSSGTFLQATDIANTGREGIDERAVRFTLRL
                     GW"
     gene            complement(7019..7459)
                     /locus_tag="VFA54_13065"
     CDS             complement(7019..7459)
                     /locus_tag="VFA54_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HZS51790.1"
                     /translation="MQIGSVVSLHIARKRSQPMMAVEQVLAVSGKGLEGDRHFGKTEN
                     RQVTLIEIEGIEALTRDYQTTILPGDARRAIVTRGVALNHLVGREFQVGEVRMRGTKL
                     CEPCDHLAQLTSETIKRGLIHRGGLCAQILTDGVIRPGDCISAD"
     gene            complement(7495..8943)
                     /locus_tag="VFA54_13070"
     CDS             complement(7495..8943)
                     /locus_tag="VFA54_13070"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZS51791.1"
                     /translation="MKPGLLVMKFGGTSVGSAERIAAAADLVSQAARTRPVVAVVSAM
                     SKVTDALLETMRRAEAGDLAGMESNIRELERRHLDVCRALFPAGQQPAVTAAVRGLLK
                     EFDRICQGILMLGEKPPRSVDEAVATGEKLSAGIVAALLECRGVPAAAVNGSEVIVTD
                     AAFGNASPILDKTRARAEKVLGPHIAANTVPVVTGFNGATADGRPTTLGRGGSDFSAS
                     ILAAVLDAEELWIWTDVDGIMTADPRVVADAAVLEEVSYNEAAELAYNGAKVLHPRTL
                     APLIEKQIPVWSKNSFRPELPGTKIVPRISDASGVRAITSLARVAMITIEAVNASMQG
                     AQLMARALDALARVNVELLLLSRSSYRQNFCMLVRSEELEMAMESLESGLALELAHGY
                     LHPIAVDESVGLLAIVGEGMRGTPGLAGRLFTAISREKINIIAIAQGSSELTIAIVVK
                     RDMLDKAVRAVHAEYRLGERKPAARQELGQAH"
     gene            9004..>9944
                     /locus_tag="VFA54_13075"
     CDS             9004..>9944
                     /locus_tag="VFA54_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS51792.1"
                     /translation="MSSPPLIFGLIGAGGIGKVRAGALRRTPGCRLAAVADTDLLRAR
                     AAATADAYCYTRYQELLDAPGIEAVIVSTPPVCHEEIVLAALAAGKHVLCEKPLATTV
                     AACRRMVEAARRSGRVLATGFNHRYFPAVKYLKDVLASGRMGTLDHVRAFAGHTGLSE
                     FNAPWMHSKEGIGGGALMDVGIHVIDLVRYVLGEVSQVYGAATHEIWKQDGAEDNGFA
                     LLRSPSGKVAALHATWTEWKGYRFFVEAYCDRGMARAYYAPMFAMAICMDKPGGRRRR
                     EFRFYPEIILREKFRGWQTTTGLAFQEELRDFTQVVRG"
BASE COUNT         1907 a         3135 c         3069 g         1833 t
ORIGIN      
        1 caaaaccgag cgactgctga tagcgcgggg agtaaaccgg actggtgagc cggccgactt
       61 cctggccccc ggcgattacc gcgcttccct tccccggcgg ctgatcaaca tcgaggccca
      121 gcctgacaag cagcttgttc acgtggccgc gggcgcggat tcgctcgacg atttcctggc
      181 ccagataaca ccccttttgg aagttcaagg cgcgttcctg ccgggtttcc tgtggcagcg
      241 cggtttcctt gaaatcctcc ccgtaaagcg gaacgccgtt ttccacgcgc acgacccgga
      301 catcctcgac cgaagcggcg acggccccgg cctgctccag ccgggctgcc agccgctcct
      361 tttcctcagc cgggacaaag agtcgaaaac cgggcccgcc ggttgccgat acacgggcca
      421 cgatcgctcc gcccgattct atatgcgcac ctggctccag ggaagcagag atgccggcca
      481 tccgctcgga cgccgggcct tccagggcca gacatgcgaa tttttccgtg acgttttcga
      541 cttgaacgtc atcggcgatg atgtgcttgt ccaggtgctg caaaacacgc tccgtaactt
      601 ccggctccac atcgagcaga atcgaatcgg gaaagacata gaggtttgca tccgccaaaa
      661 tatggccctg cgcgttcagg agaaagttgt agttgccttg ccccggctga aggctccgca
      721 cgtcgttgga gagcatggcg tgcagcagcc gcacgcgatc ctcgcccgtg aagcgaagct
      781 tgccgcgggc tgagaggtcg aaccaggcgg ccgattcatg cagagccgta tacccggcgg
      841 aaacgttcat cctcagtaat tccggcgcaa atacgcggaa agcagcagaa cgagaatccc
      901 ggagatcgcc gctccagcca tgagccgggg gcggcgcgcg tcgcgggtgg gattggcgcc
      961 cggcggcacg gccagcagca ccgccacgct gaacacgtgc agagccagca aaattttgat
     1021 tcccagaatc atgtgatacg aaggcggccg ctcagacagg cgcgtcaggt agttgtaaaa
     1081 gccggtgacc agcagcaccg caatgacggt gaaggaaatc ggccgcattc ttgttgtgag
     1141 gtcccccacc agcttgctgc gcgtctcctg cggaaggccg gccagcgaag gccctaccac
     1201 caagcgcccg taaatcagcc cgccgacgcc ggtcacggca gccgaaagat gcagccactg
     1261 catcaacgta tcgagccagt tcattgctct atactaacaa gttggcctcc aactcgcggc
     1321 gtgcgccatc gcggcacgca tgccgtggcc tagggccgcc gcccccattg cccggccgcc
     1381 cacaattctt cggggtggcg gcccaattcc atcggcccga acgccatggc tgcctcccgg
     1441 cgtggaattc cagtcacatg attcagcgcg gtatggtcac gcttggacgc cgcccgggga
     1501 ctggcagggc ctgggacgga gctcgaatgg cgcggggcgc aggaaacgcg atcactagaa
     1561 gtgcacagca caggtttttc ctatgattca caccatcttg cctgtcggaa tactgcaatg
     1621 caattgttcg atttttgggg acgagaccac gcgtgaagct ttggtcgtgg atccgggcga
     1681 cgaactgccg gaaatactaa agatcgtcgg ccagcacggc ctgcacgtaa cggccatcgc
     1741 tattacgcac gcccatattg accacatcgg cggcgcggcc gccttgcgcc ggctgaccgg
     1801 agcggcggtc tacatgaatg agcgggactt gccgatagcg aagcagctcg ccatgcaggc
     1861 agcatggctc agggttccgg ttcccgagca gccgcagatt gatgtgaccg tgaaagaggg
     1921 cgatctgatc cgcctggccg ggtgcacgtt tcaagtgctc gacacccccg gccacacgcc
     1981 cggcagcatc agcctgctgg ccgcccagga aaacaagctc ctcgccggcg atacgctgtt
     2041 tcgcgacagc atcggcagaa ccgatcttcc cggcggcgac agccgccaca tcctggcctc
     2101 gatcaagaac aagatcctga ccctcgacga ttccctaatc gtagttcccg gacacggcga
     2161 gctgacaacc gtgggaagag agcgggagca caactatttt ctaaagggcc tgtaggcgcg
     2221 ccgcagcttt atttcaaata cggcagcatc tgcttgaact cctcccggtc gctgcgggcg
     2281 agcaactcgt aaatggccat ctccgtcgaa gaaatgaccg cgccggcctc gcgcatacgc
     2341 tccagaccaa gctgcacgtt ggaagcgctg cgcgagctgg tggcgtcagc agctacgtgc
     2401 acccggtatc cggcggcaag agccccgagc accgtctgca ttacgcagat gtgggtttcg
     2461 attcctgcga tcagcagagt atgggtgcgc ggcacttcct gctccagccg tttgacgatc
     2521 cgctcatcgc ccagacagcc aaaggatgtc ttgtccatcg gtgcttccgg cagtagagaa
     2581 gccacttccg ggatggtggc gcccaggcct tggcggtatt gagtcgtagg tagcgtcttg
     2641 agtttcagga tgttcgccaa gtgcaatagc tttgtaacat tgacaagcag gcgttcctta
     2701 tcccagatgg ccggaaacag cttctcctgc aagtctataa tgaagagagc ggtggtcccc
     2761 ggccgtaaca actccaaatg agcggccata ggatcattgt agactggccg ccggcgggga
     2821 gccgtaataa agcgccagca caggaggcgg ccgttcgaga atcatagcgc gccgcctcgc
     2881 gcagccggcg gcaggctgga agcgcaggcg cggctggcgc cgccgcccgg agacagtctg
     2941 ctggtgcaaa atgggagaag gaggggagag ggcatgtatt tggacgagag ttttcgattg
     3001 aaggtcggcc tggctgagat gctgaagggc ggcgtgatca tggacgtgac caacgccgaa
     3061 caggcggaaa tcgccgagcg cgccggcgcc gccgcagtca tggcgctcga gcgcgtgccc
     3121 gccgatatcc gcaaagaggg cggcgtcgcc cgtatggcgt cgatcgccaa gatccgcgag
     3181 atcatggagc gggtcagcat tcccgtgatg gctaaagcgc ggatcggaca tttcatggag
     3241 gcccggattc tcgaagccct caaggttgat tacatcgacg agagcgaggt tttgacgccg
     3301 gccgacgaag agcaccatat cgacaagcgg agtttcaaag tcccctttgt gtgcggggct
     3361 cgcaatctcg gcgaggccct gcgccgaatc gccgagggag cagccatgat tcgcaccaaa
     3421 ggggaggccg gatcgggcaa tatcgtcgag gcggtgcggc acatgcgaac gatccagaaa
     3481 gagatgaaac agctcaccgt tctcggcaag gaggagttgg tagctgaagc gaagaagctc
     3541 caggcccctc tggaactggt ggagtactgc gccgaacacg gcaagctgcc ggtgccgaac
     3601 ttttctgcgg gcggcatcgc gacgcccgcc gatgccgctc tggtaatgca gctcggcgcg
     3661 gaagccgttt tcgtgggctc cgggattttc aaaagcgagg accccgaacg ccgggcgcgc
     3721 gcaatcgtga aggccacaac gcactacaac gatccgcaaa agctgctcga agccagtgaa
     3781 gaattaggag aggcaatgcg aggcctggat gtggcaaagc tcgaagccag cgagctgctg
     3841 cagacgcgcg gctggtaagg ccagacggcc gcaggactcg aattagcgcg agcctgaaaa
     3901 ggcgtttccc gcatgaagat cgggatttta gcggttcagg gagattttga ggcgcacggc
     3961 cgggccgtag cccgcctcgg agcaacgccg gtattcgtgc gcgccgccgg gcagttggaa
     4021 ggcctcgagg gactgatcat tccaggaggc gagagcacca cgatgctgaa ggtcctggaa
     4081 gaggaagggt tgttcgacgc catccgcagt tttgccgcac ggcggccggt gttggggact
     4141 tgcgccgggg caatactgat ggctacggag gtctcgcatc cggcccagcg cagcctcggc
     4201 ctcatcgata tggcgatcga gcgcaatgcg tacggtcggc aggtggatag ccatatttcc
     4261 aagattccgc cgacggccga gttctcgcag cgcaccggcg gccgcgagcc ggaagagccg
     4321 ctggaagccg tattcattcg cgcgccgatc attcgccggg ttggccccgg cggcatcgtg
     4381 ctgctccggc atcagaacga tccgatcctg gtggagcaag gcagccatct ggcggccacc
     4441 ttccaccccg aactgacttc ggatacgcga gtccacgggc tatttctcgc caaggccgcc
     4501 gcgtcagagc cggtttcgaa agcatgaatg gcgcaagctc gaacaatcct gtgcgtaccc
     4561 tgtttgtctg cattggcaat tcgtgccgca gccccatggc cgaggctctg gccaggcacc
     4621 tcggggccga cgtcatagag ccgagcagcg ccggcattta ccccgcctct atcatccagc
     4681 cggagacgct ccaggttctc gaagagcgag gcatcaagct ggaacctcgc aaaccgcgca
     4741 gcctgttcct gctcgacgca aaacaggtgg atttgctcgt caacatgagc ggcgcgcccc
     4801 tggacaacct gctgcattcc tttctggggc gccagatcgt atggcctgtg cgcgatccgg
     4861 tgggccagcc ccttggcgtc tatcgcgccg ttcgcgacga gatcgagaag aaggtcagcg
     4921 aattaatcaa agaactccgg caaagcggct aagttctgtt ttgtcccgat caaaacgctt
     4981 ccgcggtatt tcccgggcgc ctgcgccgcg caagggatgt tccgccaaga gagcttttcc
     5041 atttctgaaa acttccgcat tggagatttc ggctcagacc atgctccaac gggcgggcct
     5101 gctccgccaa ccttgttccc ggaggatgca cgcgattccg tggggcgcat ttaggatatt
     5161 ttcacgtggc ggccacgatc ctccgctcgt gttgacatga ctttccgcgg gtgatagcat
     5221 cgcggcggca ttttatgccg gtccatattc ctggcagagg taacgggtct cttttacgcc
     5281 ggaatccagc ggttgcagag catggcgctt tgccgcccgg gagagcaggg tgtacgcgcc
     5341 gaatccgcaa tgatccactt gcgccgttgg aaggagggtt ggccatgacc ttcaatttgt
     5401 cttttcgccg tcagtctttc gtcgtagccg tgatcttgtt cagcacactg gcgcctgttg
     5461 cttcgcgaca ggctcgcggg cagattctcc agggcaccat cgacggcaac gtaaccgacg
     5521 ccagccaggc ggcgatcgtc ggggccaggg tcgtcgcgac cgagcagcga accaatttca
     5581 cccgcgatac gaccacaaac gccggggggt tgtatacgat accgaccctt cctccccgct
     5641 gcgccaggga attcctgtgg tgacgccgcc ggatgtgagc gccggcactc tgcccgtgcc
     5701 gggcaccgcg gctctgaaca cctacgataa cgccaactac gtccggggct acatccaaac
     5761 ctggaacttt accgtcgaac aaagggtgaa aacctggatc gcttccgctg gctatgtggg
     5821 cacccgcgct gtggatccgc agaacaatat ccagatgaat tggtcgccga tcggcggcgg
     5881 aactgccggc gagatcctga atcagttgac cggccgcact gcgtccaccc aattgctcgg
     5941 cactctggga agcaatacct atgacgctct gcaaacccgg gctcagcgcc gcttctccgg
     6001 cggctatcag gtgcagttgg cttatacctt cggaaaagct ttgggctatg ccgccacacc
     6061 tgccgtttct attccccaat actaccggct gaaccgggga cctcagtcgc tcgacgtcac
     6121 ccagaatttt gaagccaccg gcatcgtgga attgcctttc ggcagaggca agcgatgggc
     6181 gcagagcggt atagccgcca agacggcggg cggctggcag ctcagtaccg tcttcagcgc
     6241 ttacagcggg cgtccgttca ccgccacggc ctcgacggca acgctgaatt ccccgaactc
     6301 gagccaattc gccgattgca tcagcgcgcc tcaccagacc ggcaatatct tccagtggta
     6361 tgacaagtcc gccttcgccg tgccggcttc ggggcgcttc ggcacatgcg gtacaggcag
     6421 cctgaggggg ccgggcctgg ttaatgtgga tctcgggcta gaccgcaagt tcccgattcg
     6481 cgagcgattc gagctccggt tccgggcgga aatgttcaat gcggccaaca cgccgcacca
     6541 tgtcatgccg ggcggaaacg cttcggtcag cagcggaact tttttgcagg ctacggatat
     6601 tgcgaatact ggacgggagg gcattgacga gcgggcagtc cgcttcacgc tgcggctcgg
     6661 ctggtagcgg cacggcaggc cggatggagc tgtcccggcc cgccgggcag actctgggga
     6721 ccgcatagtc tagctcatcg cccacacggg gcgcggctta tccttgtggc tgctgcggct
     6781 cgcgcttgcg gtggggcgca gtgcatacat aacgttcccg cttgccaagg gctgtattat
     6841 ccgaccctcg tcacgctccc atcgctcgtg ggggcgtgac gaaaaatcga ggttcgcatg
     6901 tgccggagac ccaacgacga gagcacgtgc tcagcgttgg ggcgccagcc aagcccgcgc
     6961 cggcgcttcc taaactcttt tcgtgcagcg ggagccgaag gaaagcggag agctgcgtct
     7021 aatcggcgct gatgcagtcg cccggccgga tgacgccgtc cgtgagaatc tgagcgcaca
     7081 ggccgccgcg gtgaatgagg cccctcttga tggtctcaga ggtcaattgc gccaggtgat
     7141 cacaaggctc acaaagcttc gttccccgca tccggacctc gccgacttga aattctctcc
     7201 ccacgagatg attcaacgcc acgccccgag tgacgatcgc tcgtcttgca tcacctggca
     7261 ggatagtggt ttggtagtcg cgagtcaggg cctcgatgcc ttcgatctcg atcaacgtca
     7321 cctgccggtt ctctgttttt ccaaagtggc ggtcgccttc caaacccttg ccggaaacgg
     7381 cgagcacctg ctcgaccgcc atcatgggct ggctgcgctt ccgcgcgatg tgaagggaaa
     7441 caaccgatcc gatttgcata ggcgctcctt cccgactccg gcaatttttc ccgcttagtg
     7501 cgcctggccc agctcctgcc gggcagccgg cttgcgttcc ccaagccgat actcggcgtg
     7561 gactgcgcgc accgccttgt ccagcatatc gcgcttgacg acaatggcga tcgtcagttc
     7621 gctcgacccc tgcgcgatgg cgatgatatt gattttttcc cgggaaatcg cggtgaacag
     7681 gcggccagcc aggccgggtg tgccgcgcat accttcgccc acgatcgcca gcagccccac
     7741 gctctcatcc actgcaatcg gatgcagata gccgtgcgcc agctcgaggg ccagccccga
     7801 ttccaggctc tccatcgcca tttccagctc ttccgagcgc accagcatgc agaaattctg
     7861 ccggtagctg gaccgcgaca gcagcagcag ctcgacattg actcgagcca gcgcgtccag
     7921 cgcgcgggcc attagctgcg ccccctgcat cgaagcattc accgcctcga tggtgatcat
     7981 agccacgcgg gccagcgacg tgatggcccg cacgcccgag gcatcgctaa tccgggggac
     8041 gattttggtc cccggcagtt cgggacgaaa gctgttcttg ctccacaccg gaatctgctt
     8101 ttcaatgagc ggcgccagcg tccgcgggtg gaggactttt gcgccgttgt aagccagttc
     8161 cgccgcttcg ttgtagctca cttcctcgag caccgccgcg tcggcgacca ccctcggatc
     8221 ggcggtcatg atgccgtcca cgtcggtcca gatccatagc tcctcggcat ccagcacagc
     8281 ggccagaatc gacgctgaaa aatccgaccc gccgcggccg agcgtcgtcg ggcgtccgtc
     8341 ggcggtggcc ccgttgaacc cggttactac cggcacagtg ttcgccgcga tgtgcgggcc
     8401 taaaactttc tcggcgcggg cgcgtgtttt gtcgaggatc ggcgaggcat tgccgaaagc
     8461 ggcatcggtc acgatgacct ccgaaccgtt caccgccgcg gcgggaacgc cccggcattc
     8521 gagcagggcg gccacgattc ctgccgagag cttttcgccc gtcgcgacag cctcatcgac
     8581 cgaacgcggc ggcttttccc ccagcatgag aatgccctgg cagatgcggt caaactcctt
     8641 caacaggccc cgtacggccg cggtgacggc gggctgctgc ccggcgggaa acagcgcccg
     8701 gcagacgtcc aggtgccggc gctcgagttc gcgtatgttt gattccatgc cggccagatc
     8761 tccggcctcg gctcggcgca tcgtttcgag cagcgcgtcg gtgaccttgg acatcgccga
     8821 aaccaccgcc actacaggcc gcgtcctggc ggcctgcgat accagatcag ccgcggcggc
     8881 aatgcgctcc gcgcttccca cgctggttcc gccgaacttc atcacgagta aacccggttt
     8941 catcccgctc ctgccgctgc aagccccact gggcctactg gatagcatac actgggggga
     9001 gggatgagtt ctccgcccct tatctttgga ttgatcggcg caggcggtat cggcaaggtg
     9061 cgagccggcg ctttacgccg gactcccggc tgccggctgg ctgcggtggc cgacacggat
     9121 ctgttgcgcg cgcgggcggc cgccactgcg gatgcgtact gctatacaag gtatcaggaa
     9181 ttactagacg cgcccgggat cgaggcggtg atcgtctcca ctccccctgt ttgccatgag
     9241 gaaatcgtat tagcggccct ggcggccgga aagcacgtgc tgtgcgaaaa gcctctcgcc
     9301 acgaccgtgg ccgcctgccg ccggatggtg gaagcggccc ggcggtccgg ccgcgtcctg
     9361 gccacaggct tcaatcaccg ctactttccc gcggtgaaat atctcaagga cgttttggcg
     9421 agcggccgca tgggcacgct cgatcacgtt cgcgccttcg ccggtcacac cggcctgagt
     9481 gaattcaacg cgccgtggat gcacagcaaa gagggaatcg gcggcggggc cctgatggat
     9541 gtgggtattc acgtcattga tttggtgcgc tacgtcctgg gcgaagtcag ccaggtctat
     9601 ggggccgcta ctcatgaaat ctggaagcag gacggcgccg aagacaacgg cttcgcccta
     9661 ctgaggagcc cttccggcaa agtggcggct ctgcacgcca cctggaccga atggaaggga
     9721 tatcgctttt tcgtggaggc ttactgcgat cggggaatgg ctcgggctta ctatgcgccg
     9781 atgttcgcca tggccatctg catggataaa ccgggcggcc gccgccgccg cgaattccgc
     9841 ttctaccccg agatcattct gcgcgagaaa ttccgaggtt ggcaaaccac cacaggtctt
     9901 gccttccaag aggagctgcg cgattttaca caggtcgtcc gcgg
//
LOCUS       DASREK010000246        33485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1765580, whole genome shotgun sequence.
ACCESSION   DASREK010000246 DASREK010000000
VERSION     DASREK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 33485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33485
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765580"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1578)
                     /gene="purH"
                     /locus_tag="VFA54_13080"
     CDS             complement(28..1578)
                     /gene="purH"
                     /locus_tag="VFA54_13080"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZS51793.1"
                     /translation="MVKIQRALLSVTDKTGLTEFAQVLARLGVELISTGGTARLLREA
                     GIAVREVSDVTGFPEMLGGRVKTMHPRITGGILAVRSNQEHMNALRRHDIAPIDLVVV
                     NLYAFEKTAAKPGVALEEIVENIDIGGPTMIRSAAKNFADVAVVTDPRDYAAVAAEMQ
                     TGGGCLSEATCWRLAQKAFALTAAYDRAIANTLANLHSSGEAGLAIEPLPAFLDIRTP
                     RRMSLRYGENPHQSAALYAEGDAGVAGARQLHGKELSYNNLVDLDAAWQLTREFEDPA
                     CAIIKHTNPCGCAEQSTVLAAYRKALACDPVSAFGGVLAFNRPLDGETAAAITELFVE
                     AIAAPAYSDEALERLKTRKNLRLVEVSPAPSTLVVKSLSGGYLAQTEDCITLDRKNLK
                     VVTDRAPTQQETSALEFAWKVAKHVKSNAIVFAVAGQTVGVGAGQMSRVDSVKLAASK
                     AVLPLKGTVVGSDAFFPFPDGVEELARHGATAVIQPGGSVRDADVIAAADRLSLAMVF
                     TGMRHFLH"
     gene            1895..2698
                     /locus_tag="VFA54_13085"
     CDS             1895..2698
                     /locus_tag="VFA54_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZS51794.1"
                     /translation="MKLTERRRLDRLPASGSGTLEWIDPSRRRRTATVTVKDITADGV
                     RLEADQPLETWNGARLIGETWECLGSIRYCRPENGRFVAGMQLTRLPIRRTDSRGPQL
                     VKQKQTTLTRPAQKLPAGRPAAGKEDPVGPAAPKAAAKPADPRNPRPSSAPISALKGE
                     IRKGEAFVSADSDVIPDRRRSERTPTGGWATLDCGPHCVTVRVTNLSDEGATIELAGP
                     VEAPQMARLTGEVWQCLGWLRYCKPAGEKYVAGFQFTRSYPKNSCDYFD"
     gene            complement(2800..4734)
                     /gene="thrS"
                     /locus_tag="VFA54_13090"
     CDS             complement(2800..4734)
                     /gene="thrS"
                     /locus_tag="VFA54_13090"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZS51795.1"
                     /translation="METIRVKFPDGSERSFPKGITPLDIARELGPRLAEAALVAKIDG
                     RLIDLKSPLMEDTPLRILTSKDPEALEVYRHSSAHVLAAAVLELYPETKLGIGPPTEE
                     GFFYDFQRETPFTPEDLAKIEARMRELVARDLPFERVWLPREESLKKFREMGEEMKCE
                     LIEEKAEGDRVSCYTTGPHFIDFCRGPHIPSTGKIKALKLLSIAGAYWKGDEHNARMQ
                     RIYGTSFFTQQELDAYLQRIEEAKKRDHRKLGPELDLFSIQDAAGPGLIFWHPKGGIV
                     RKVMEDWLREQYLRRGYGLVFTPHVAREELWKISGHLNYYAENMFAAMELDDARYRLK
                     PMNCPFHILIYKDRLRSYRDLPVRLGELGTVYRYERSGVMHGLMRVRGFTQDDAHIFC
                     TPGQIKDEIAGCLDFALEVLRTFGFQEYKVELSTWDKGASGKYDGTPDQWGLAETALL
                     EALQQLGVEARIIPDEAAFYGPKIDVKLVDAIGRLWQLSTVQFDFTLPRRFGLEYIGE
                     DGKAHQPLMVHRALWGSVERFFGVLLEHYAGAFPLWLAPVQAVILPITDKQLDYARSV
                     ASRLQAAGLRVGVDARSEKINAKIREAQLQKTPYMLVVGAREVEKQQVAVRHRKHGDL
                     GAKALDEFLAEAQKEIAEKT"
     gene            complement(5216..6100)
                     /gene="galU"
                     /locus_tag="VFA54_13095"
     CDS             complement(5216..6100)
                     /gene="galU"
                     /locus_tag="VFA54_13095"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZS51796.1"
                     /translation="MNCRVRKAVFPAAGLGTRFLPVTKSQPKEMLPLVDKPIIQYGVE
                     EAIDSGIENIIIVTGRGKTAIEDHFDVSFELEYLLESRGKLKELEIVRHVSELIHVAY
                     IRQKEALGLGHAVLVTRELAGDEPFAVVLADDVIQSARPCLRQLLDVFEHTGCCVLAV
                     MEVPREKISAYGVIAGDAEPFNGSRDRLFRVRDMVEKPPAEKAPSNLAIIGRYVLVPE
                     IFSTLAATKPGSIGEIQLTDGIRQLLDRQPVYAYRFEGTRYDAGDRLGFLKATVEFAL
                     RREDLGTEFRQYLKSLEL"
     gene            6611..7531
                     /locus_tag="VFA54_13100"
     CDS             6611..7531
                     /locus_tag="VFA54_13100"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZS51797.1"
                     /translation="MSRGVGVAVWAAALVWAAEAPDPPKAAPQVYTVGVDARTGKLIR
                     IPAPTKSSPFVTTRRAVSRKSSYETAAANPPGTEVERGAASQEATALQPDRASSQVEP
                     SPPGKTLVVIDEPNQRTEDAEKGPEAAGGRRPAAHNVVRPAAFNGLVERVALRYDINP
                     GFVHAVIKAESDYNPRAVSRKGAVGLMQLTPLTARRFGVSNLYNPVENLEGGVRYLRH
                     LLDLYNYDPKLSLAAYNAGEGAVDRFRGVPPYSETRRYVRRVSEIYSRSLVWLAPAPA
                     PKKIEGPRIYRVVEPSGTVRYVTEPQPESQ"
     gene            7537..9720
                     /locus_tag="VFA54_13105"
     CDS             7537..9720
                     /locus_tag="VFA54_13105"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZS51798.1"
                     /translation="MPKTANRRRVFALVPGVVALIAGSPFLHGASAKRELALQYFQQA
                     QQQRLDLESVREADRTRENYQKVILAFRRVYTTSPQSSKADDSLLAVGDLYSEMSERF
                     GPAPYRDKAIQAYRFLINEYPLSPLLEDARQKLQALETQASGGRPAPPAVPRAVAASL
                     ASAGEAAGAGSGLRISDVRYFSFPELTRVVITLDADVDVHSERLTDPDRVYFDLPNTW
                     LSPAWQGRSIHVGDHLVRRIRIAHTRVGVTRVVVDLNADAQYAISKLTNPARIVLELR
                     RRDAGIKPFLAGADAERRNLESAPQGPLRKDGSAAQPAAAKTQGPSAASAIRASEPPR
                     LAEVPQNTSRSPRSLDGRAADSGDASSPSPPKEAAKKEPAAVPGKTTTVFNSGDFADS
                     RRPRPGIDSPKPAGAVVAPIAAAAAGVPTPSPAALPAQSRPVAPALPAAPLESRPTVE
                     QTSAAAVDSRAAPAKPAASPALSKLAPPKPAQPTSSGERDLIRALGLKISRVVIDAGH
                     GGHDTGTVGPTGLMEKDLTLDIATRLGALIAERIGSEVIFTRTDDTFIPLQDRTALAN
                     EKGADLFISIHANSSRVRAVRGIETYYLNLTGDREALETAARENAASQKSIHELQDLV
                     SQITLTEKISESREFASQVQRSLYSAVSRETGALRNRGVRTAPFVVLIGARMPSVLAE
                     VSFLSNPRDEKLLKTGAYRQKIAEALYKGLASYAGTLSQVEVAKK"
     gene            complement(9735..11813)
                     /locus_tag="VFA54_13110"
     CDS             complement(9735..11813)
                     /locus_tag="VFA54_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HZS51799.1"
                     /translation="MPETLHSVCSHDCPDACSILVDVDKGRAVRIRGNPDHPVTRGFL
                     CGKVARYLDRVYSPDRVLYPMRRVGAKGQGRFERIGWEEALDSIAARLQSIIAAYGPE
                     SVLPYSYGGTMGYLNGAGMDRRFFHRLGASLLDRTICSEAGAAGLAATQGVRVGTEPE
                     QFRHSKLILAWGANILATNVHLWPFIVEARRAGARFYVIDPHQNRTSRQADWHIAVYP
                     GSDTALALAVLHVIIGEGLYDADYVARYTLGFEDLRERVKEYPPQKAAALTGVAAADI
                     VRLAREYATTRPAAIRLNYGLQRSERGGMAVRTIAMLPAITGSWREIGGGLQLSTSQA
                     FRLRRDRLEMPELWLRSPLGRKPRTINMVELGKALLEADSPPVKALFVYNSNPAAVAP
                     DQTRVLRGLRREDLFTVVSEQFQTDTADYADFLLPATTFLEHTDLYFAYGHYHLQLAR
                     PAIAPLGEAISNVELFRRLARRMGFDDPCFRETEEEMLRALLSSDHRWARGITLEQLD
                     REHSVRLPVAPAGEPFLPFAQGEFETPSGKCELRADSLVKQGIDPLPSYMPPVESRFG
                     DAELRGKYPLEMISPKCPDRVNTTFGHGKPGSEPGFCVEIHPDDAALRGIADGDMVRI
                     FNQRGSCRLAAAVSATVPRGVIASLSGRWNKLSPDGQNVNALVSGRLTDMGGGPTFYS
                     TLVEVERAGD"
     gene            12099..12656
                     /locus_tag="VFA54_13115"
     CDS             12099..12656
                     /locus_tag="VFA54_13115"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS51800.1"
                     /translation="MRRLLLLSILCLAPAWAAGELSNRRAPGFSLPDSNIQQHDIADY
                     RGKIVLLELMQTSCPHCVKFAGVLEEISRKYAGRVQVLSIVTPPDTTATVGPFIRQHN
                     ITYPILFDCGQATGSYMMATPQKPSFDIPHVFLIDQEGIIRNDFGYGPLTTAIFEGRG
                     LFAEVDKMLASPGSGAPPKAAPKKP"
     gene            complement(12705..13784)
                     /locus_tag="VFA54_13120"
     CDS             complement(12705..13784)
                     /locus_tag="VFA54_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZS51801.1"
                     /translation="MNVGLDATYSLGRALSGVGVYCRRLIEEIVAAAPEFHFILYYRP
                     HRFFRSLLEQRWPSNVSRRLLEEPLNVFLPRRVAVFHGLNQRLPRYSFRQAITTFHDL
                     FALTGEYSTPEFRERFAALARDAAARSGLVIAVSCYTAHCVEALLGVESSRIVVIHHG
                     VDPIPEFAAEDLKAFRRRFRLERPFLLHVGALQARKNILRLIEAFETLEGDVDLVLAG
                     SDGYKAEEIHARIANSPARQRIRALGYLPNGAIERLYRSAAALAFPSLEEGFGLPVLE
                     AMSAGLPVVTSNCSALAEIAGGAALLVDPLDTAALAEGLSRAAADSSLRRELIEKGRA
                     RAAQFTWRRAAERTRDAYRQFRGCE"
     gene            complement(13781..15286)
                     /locus_tag="VFA54_13125"
     CDS             complement(13781..15286)
                     /locus_tag="VFA54_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZS51802.1"
                     /translation="MHPQKAAAACAEFQKLRDQDLLTPAETARLAAHAAQACLASAAE
                     LDLAVPLLAEIATLADAHLASSGTDAIFRQVVEPLADAFDPACCELYIRFMARLLECC
                     RRLSNAAWLEAQLTRFGISNEGDLIRRALSLRRFGRAAVEAGAIEKALVLSRVTLGAD
                     VAVTSVVLRKMMQVFPNADLVLVGSDRAALLFAGEPRVRARRASYLRGGGLLDRLAAW
                     PAVVAIVGEEIKGLPAGRYVIIDPDSRLTQLGMLPLTAGDAGYLFFESRSYAKPGREA
                     LSDLTSAWLDDAFGPGTAPARPWVSPPAAALRLAESLRAVLRSPAHREEGSGSAPLRG
                     WISINFGVGDNPGKRIPGNFEYLLLRELLGRGWRVFLDRGDGAEESARTGSLLARLRE
                     SGTRIVEIEEREPLPPSAGAQIIAWRGSLAGFAALIRLSDLYIGYDSACQHIAAALGK
                     KTVDIMAGFRSPRILQRWRPCGPAEVKTVVVSPEAPAHPERIVDVVLEAVS"
     gene            complement(15392..16396)
                     /locus_tag="VFA54_13130"
     CDS             complement(15392..16396)
                     /locus_tag="VFA54_13130"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS51803.1"
                     /translation="MQEPPSESAAYPFGLVGDSPEVQRVLRIIYKLANNRSPVLVTGE
                     SGTGKELVARALHAVAPGTRGVFVAVNVPALPASLVESELFGHLRGSFTGATAARRGL
                     IEEASGGALFLDEIGEMPLELQPKLLRVLETQEVRPLGAEHAEKIDVRVIAATNRDLG
                     KLVEEGKFRADLYYRLNVVALRLPPLRERREDIPLLIRYFLQRYAPRPMTMTPLSLRA
                     MLEYDWPGNVRQLENAIRRMVALCSGNELHLGDLPTQIRNAAGALQAEPRLVALPEVA
                     PLANVERLAIEHAMKVTRGDRRKAAKLLGIGKTTLYRKLKGYGLDTKAPDAKVNDAGL
                     "
     gene            complement(16408..16956)
                     /locus_tag="VFA54_13135"
     CDS             complement(16408..16956)
                     /locus_tag="VFA54_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZS51804.1"
                     /translation="MASWYGHPYHGRPTTSGEIYDMEKLTAAHRTLPLGARVRVENLS
                     NGRTVEVRINDRGPFDKDRIIDLSHAAARRIAMIGPGTARVRLTVIGLPETPSPGFYA
                     VQVGSFQSRGNAERLRSRMAREHGAAVIRSYESGRGLFYRVLVGHESDWNAANALVEK
                     LRRENFTPLVIRIDELPANAGL"
     gene            complement(17088..17297)
                     /locus_tag="VFA54_13140"
     CDS             complement(17088..17297)
                     /locus_tag="VFA54_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51805.1"
                     /translation="MGEKSPRSDAFEQAAREIEAEVKRALDFIEGRVVPRARRDGEKL
                     LRRLSEELSRWADRLHGEPPEEERK"
     gene            complement(17391..18641)
                     /locus_tag="VFA54_13145"
     CDS             complement(17391..18641)
                     /locus_tag="VFA54_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZS51806.1"
                     /translation="MYTRRVFLKNAAFAMVGVGSAPAWLARTVFAETYSGRRKKVLVA
                     IFQRGAADGLNIVVPFGEKSYYSLRPTIAVPRPGVSEEDSAIDLDGFFGLNPALKPLV
                     PLYRGAHLAIVHAVGSPDPTRSHFDAQDYMESGTPGRKATPGGWMNRALAPERGTVSP
                     VRAVAVGPTLPRALRGETPALAINDLPGFQIRDPGAAVPFETMYQTTGDTLLRGAGRE
                     TFEAIRLLSSIEKRPYSPENGASYPGGRFGQSLQQIARLIKADVGVEVAFVDIGGWDH
                     HVNEVGARASAGQLPNLLREFGRSLAAFWQDLGDRMADVALVTMSEFGRTARENGNRG
                     TDHGHANVMFVMGGDVRGGKVYGEWPGMEKEQLYEGRDLNLTTDFRDVLGELVSRHLG
                     NPNLNAVFPGYAADARKFRGLIPT"
     gene            complement(18652..20544)
                     /locus_tag="VFA54_13150"
     CDS             complement(18652..20544)
                     /locus_tag="VFA54_13150"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZS51807.1"
                     /translation="MRIYKPTFLGLGLLLFLAAGPAKLPGAKKDDAFSKKLSSEQKIA
                     HALNRLTFGPRPGDFERVRSMGLKKWIDLQLHPERIAENPALEAKLRVLDTLRMSTSE
                     LVENYPPPQVIRAMAMGRLPYPQDPEKRKRLQRLTERYERRFEQQGQAEQPEARRQPA
                     IAGMAPLANLLSPEERNAFRQSTPEERLKLFFSLPEEKQEDLIEVLPANMRRAVIARA
                     PDDLRRRLLRSLAPQQVVVSDLVESKLYRAIYSNRQLEEELVDFWFNHFNVFLDKGAD
                     RYLVTSYERDAIRPHVLGKFKDLLLATAQSPAMLFYLDNWQSVDPAAAERMLNGRRRL
                     LTFQKALPRRGLNENYGRELLELHTMGVDGGYTQRDVIEVARCFTGWTINRPQLGGTF
                     VFNEGMHDRGEKTVLGVKIPAGGGLSDGLKVIDILASHPSTAHFISRKLAQRFVADNP
                     PDSLVEKMARTYLKSGGDIREVMKTMLASSEFWSQGAYRAKVKSPLEIVVSAVRGLDA
                     EVNFAAALDFQTAQLGEPLYRKQEPTGYSNASQDWINSAALLARMNFAEALTRNSLPG
                     IRVDPNRVAQTAAADPADVARELLLADPSNATREAITRSDTAEGGLQPLQVAGLVIGS
                     PDFQRK"
     gene            complement(21439..22218)
                     /locus_tag="VFA54_13155"
     CDS             complement(21439..22218)
                     /locus_tag="VFA54_13155"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZS51808.1"
                     /translation="MNRRSFVQSLGGATMLSALASDPAQAQGRKTRLYRIEYFYFRQG
                     DQGTRLNRFLSSQMPLLTKNIRTLGVFNSLIGPHVPATVILAGFSGYEEMEAARKRIT
                     QDPGYQKAHEELENGSEPPYDYADQALLEATDFSPEIVPLKEKPKTPRVFELRVYHSP
                     TQRQLKLLLERFGGPEIKIFHTVGIHPILYTTTVIGQNMPNLTYLIPFASLADREKAW
                     DAFGASPDWIKARDESIARGGQIVSQSNITLLRPAAFSPIQ"
     gene            22600..22676
                     /locus_tag="VFA54_13160"
     tRNA            22600..22676
                     /locus_tag="VFA54_13160"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22634..22636,aa:Val,seq:gac)
     gene            23340..24158
                     /locus_tag="VFA54_13165"
     CDS             23340..24158
                     /locus_tag="VFA54_13165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS51809.1"
                     /translation="MTTLRGPVISILAAGLTIGAAIPAAQPQATGQSVTQQAITQQSA
                     SRQTTSQQTVSQPTTSQPVITPTAKELNQSFNADAAQWAARFEHEGRAIYDKRFEILD
                     AMGLKPGMDVADIGAGSGLISRLIAQRVAPGGTVYAVDIAKNMVDHIARTAQEQKITN
                     LKAVLGDPHSPQLPPNSVDAVCIIDAYHHFEYPKDMLAEINKALRPDGLLLLIDFKRI
                     EGVSRPYILKMVRAGEGTFTDEFLNAGFELIERREDMFPENYLLKFRHRRPRAN"
     gene            24428..26884
                     /locus_tag="VFA54_13170"
     CDS             24428..26884
                     /locus_tag="VFA54_13170"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZS51810.1"
                     /translation="MQASIFAFLVGVTGSPSAAQRSASPARAQAPFTAAESRAVFDKY
                     CAACHNAKLNTAGLALESLDVTKPSANPEKWERVIRKLRTGTMPPRGLPRPDEATYDA
                     VASWLEMDIDRAAAAHPNPGRTNSVHRLNRTEYHNAIRDLFALDFDVNSQLPGDETAD
                     GSFDNNADVLGITRSHLERYMSVARQITRLATGLPPASPVVETFKVPLHVVQDDRQSE
                     DLPFGSRGGLAVRYDFPADGDYTFKVRLRRQYQDYIMGMGWPQRLEIRLDGKLLKRFT
                     IGGQAPGKPSPASFAGAGNSFGDPEWEKYMQGADDGLEIRVPVKAGPRVVGVDFVREL
                     WEPELVPQPLQRGRVITDDEIYMDYASVGSVQIGGPYQVSGPARDTPSRRAIFVCQPQ
                     TAAEEKPCAVRILSRMARRAYRRPVTDRDVQTLLEFFDSGRRDGKSFDAGIQFALERL
                     LVDPDFLLRVLRDPPQAKPGEVYRLSDLDLASRLSFFLWSSIPDEQLLDLAEKGKLSD
                     PKILEPQVRRMLANSRATDALVNDFAAQWLNLRRVGDVVVDPRYYPNFDESLLEAFKE
                     ETQMFVASTLREDRSVLDLVKADYTFVNERLARHYGIPGIYGSRFRRVQLPNLDQRGG
                     LLAEGALLAATSYPDRTSPVLRGKWLLDNILGMPPPPPPPGVNTTLQEPKRGARPASI
                     RERLDQHRHEALCASCHSQIDPMGFALENFDVLGGWRTKDEAGNPIDSTGTWPSGRKI
                     DGFTGLRAMLLDHPDSFVGTLTEKLMSYALARRLDYYDKPAVRKIVRDAKANDYRWSS
                     IILGIVQSPPFQMRSAKAGT"
     gene            27025..28353
                     /locus_tag="VFA54_13175"
     CDS             27025..28353
                     /locus_tag="VFA54_13175"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZS51811.1"
                     /translation="MTRKSLPRRTVLRGMGISLALPLLDSMFPAFSRAGTIAKPVHRF
                     QAVYVPNGMAMEYWSPKTVGKDFELTPILEPLAPFRNQMMVLSGLHANWNYIHAGASG
                     SFLTGTTRGGRTEVEIIADVSMDQLLAKKFANETQLPSLELSMDAPNNAGACTGALSC
                     AYTHTISWRSPTQPLPMEHNPRVVFEKLFGDTGSTDRAAREARLRQHKSILDSVTEKL
                     ADLKREVGPQDQVKVDEYTEAVRDVERRVQKAEQQTNIELPVMEQPQGVPTVFEDHLD
                     LMFDLQLLALRTDLTRVITFMIGKEQSARPYPQIGVPEAHHPLSHHNNQPDVIARMSK
                     INRYHTELFSKYLAKLRATPDGEGSLLDHMTILYGSGISNSNQHSGENLPILLLGGGA
                     GKLKGGRHIKYSDRPSMANLLVTLMEKLDAPVERIGGSTGKLPLDTLSDV"
     gene            28452..29861
                     /locus_tag="VFA54_13180"
     CDS             28452..29861
                     /locus_tag="VFA54_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZS51812.1"
                     /translation="MTARWLRLLALGLAVTLVEAAGSSLALIQAARNTDKDALHALLL
                     KPGVEVNAAEGDGSTALHWASYRDDLEGADLLIRAGANVNAANDLGVTPLWMACQNGS
                     AAMARKLLDAGADPNAKLLSGETLVMTASRSGKAEVVELLLARGADVNARATRGQTAL
                     MWAAAQKHPEVVKVLLAHGADVKARSDVWTQLWQTAPDQDVHPDYQVRLKLGGDTPLL
                     FAARVGDLASAKLLIEAGADVNDASAYGTSATVLAVHSGNAELVEYLLEKGADPNAAG
                     AGYTALHAAILRRNTRAVAALLAHGADPNAKLLASTPVRRSSHDFYFHPAYVGATPFW
                     LAARFTQPEVMRMLAEHGADPLVVHNVSFWGRRTKTSEYKREAPGPTTALMAAVGMGR
                     GSGFAAPEPAQREALTLEAVQIAVELGVDVNAADADGRTALETATALKYKSVVEFLTS
                     KGAKLDHPARPLPKEAVDN"
     gene            30021..30860
                     /locus_tag="VFA54_13185"
     CDS             30021..30860
                     /locus_tag="VFA54_13185"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZS51813.1"
                     /translation="MLPFGVAVSRRTILRAAAAAAASGVLRPASGQQRGPRVLALIGD
                     RYHNADYIQVALTRMFDGLGLVVDYTIDYKQLSRKVLRDYQIFLCLRDGMIWPNGYLG
                     PDAYTSYEQGLENRGDFPEAKSQSWLTEEQAVALKDFVTEGKGFYSLHNNSHVSLSSR
                     TYREVMGGAYIGHPPLRPFQVHVVNSNHPITQGIHDFMVNDEQHYVEYDKDPKYVLLE
                     AENIDGLTFRNLGAKSIAGWAYDYGRGRVVFTAVGHTIHALWAPEYLKLQRRAVQWLL
                     KEI"
     gene            complement(30962..31324)
                     /locus_tag="VFA54_13190"
     CDS             complement(30962..31324)
                     /locus_tag="VFA54_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZS51814.1"
                     /translation="MPGRRRPQGYYKRVWEIVRLIPSGRVSSYGAVARAAGFPGTARQ
                     VAWALRGSPRSANLPWHRVIGAGGKILLSGEAGIEQRLRLQAEGVAFRGARVQMDRHE
                     YRFSRGGGRPQKRKVSGR"
     gene            complement(31314..33023)
                     /locus_tag="VFA54_13195"
     CDS             complement(31314..33023)
                     /locus_tag="VFA54_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS51815.1"
                     /translation="MNGHILLLLLLMLALSGSTLAFSTWRFRRRNAAQQRQLSNLYAV
                     AEDMIAAAEPAEIYRCIVRTLPAMLDATHGYLFLQNRVTKQLDVLAGTDQFPAAGCSM
                     DVVSGPVTCFRNKALLEVPDAENCPFLDHSVVEQLRQKSVLFVPLISEGETFGVLEID
                     DRRRKRSFTEEQKASAQHVANLGALAMRLCEQRSMREQLYRTEKMAAVGELISGVAHE
                     LKNPLASLSGLSELALARYGSGPLAQDLRAIHAEARHANTILQRLISFARPQKSGPVL
                     VDINAVLRSVVAIRAEKWESQGIRVHVQLSTTPPIVASDQSHLEQVFLNLLINAERAL
                     EGSREKVITVRTTIAAKRVLIHISDTGPLFSADLQSRVYHPFFDARKPIETAGLGLAL
                     CQSLIEGHGGSIRVSNSPNHATTFEIEYPLADSLPPKPAQVPAARKTESRPPATLTAL
                     VIDEDRKVQDSLLSLLSDRQYRVITVSSAEEALDLAERARFDLVLCDVRMRGISGIEL
                     YRRLQSRIQSFVFLTADTFSTDIREMFSEPNQAVLAKPFTAADVERLLDEIEPRMTGV
                     HAG"
     gene            33227..>33485
                     /locus_tag="VFA54_13200"
     CDS             33227..>33485
                     /locus_tag="VFA54_13200"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZS51816.1"
                     /translation="MEAILVERFAAGFAALGNPARLRIVRLLLAAYQSGGMTAGKIQA
                     ELGIPASTLTHHLAKLESAGLIRSQQDRQWIWYSIDAKGLRE"
BASE COUNT         6656 a        10422 c        10105 g         6302 t
ORIGIN      
        1 gccaactgtt ggctgcgtac ttcctgctca gtgcaaaaag tggcgcatgc cggtgaagac
       61 catggcaagg ctgaggcgat ccgccgccgc gatcacgtcg gcgtcgcgga ccgagccgcc
      121 cggctggatc acggctgttg ccccgtgccg cgccagttcc tccacgccgt ccggaaaagg
      181 gaagaaagcg tcggaaccta ctaccgtccc tttaagcggg agcaccgcct tgcttgcagc
      241 cagtttcacc gaatcgacgc ggctcatctg ccccgctccc actcccaccg tctgacccgc
      301 gacagcaaaa acgatcgcgt ttgacttgac gtgcttggcg actttccacg caaattccag
      361 agcggacgtt tcttgctgtg tcggcgcgcg atcggtgacg actttcagat tcttgcgatc
      421 cagagtgatg caatcttcgg tctgagcaag atacccgccc gagaggcttt tcacaacgag
      481 agtcgagggc gcaggtgaca cttctaccag ccggagattc ttccttgtct tcagccgctc
      541 gagagcctcg tcggaatagg ccggagcggc gattgcttcc acgaaaagct ctgtgatggc
      601 ggcggcagtt tcgccgtcca gcgggcggtt aaaggccagc acgccgccga aagccgaaac
      661 gggatcgcag gcaagcgcct tgcggtacgc cgccaggact gtgctctgct cggcgcagcc
      721 gcaagggttg gtgtgcttga tgatcgcgca ggcaggatct tcgaattcgc gcgtgagctg
      781 ccaggccgca tcgagatcga ccaggttgtt ataggagagt tctttcccgt gcaattggcg
      841 cgctccggcg acgccggcgt cgccctccgc atagagcgcc gcggactggt gtggattttc
      901 gccatagcga agactcatgc ggcgcggcgt ccggatatcc agaaaggcag gcagcggctc
      961 gatagcgagg ccggcctcgc cggaagaatg aagatttgcc agcgtattag cgatagcgcg
     1021 gtcatacgcg gccgtgaggg cgaacgcttt ttgggccagg cgccagcagg tcgcttcgga
     1081 aaggcagccg ccgccggtct gcatttccgc cgcgacagcc gcatagtcgc gagggtccgt
     1141 cactacagcg acatccgcga aattctttgc cgccgagcgg atcatcgtcg gcccaccgat
     1201 gtcgatattt tccacaatct cctccagagc cactcccggc ttggccgccg tcttttcgaa
     1261 ggcgtacagg ttgactacga ccaggtcgat cggcgcgatg tcgtgccgtc gcagagcatt
     1321 catgtgctcc tggttggagc gcacggccag aatgccgccg gtaatgcgcg ggtgcatcgt
     1381 tttcacccgg cctccgagca tttcaggaaa accggtcaca tcggagacct cgcggacggc
     1441 gatgccggct tcgcgcagca atcgagccgt gccgcccgtc gaaatcagct ccacgccgag
     1501 gcgcgccagc acctgcgcga attcagtcag gccagtcttg tcggtaacgc taagcaaggc
     1561 tcgttggatc tttaccatgg cgggaaagaa accgattttc tcacaacagt tggcccttgt
     1621 ataaacgggg ccctcgaaag gcagccctca gcgtgaacaa aagggtcgcg cccccagctt
     1681 cgcacaggca tgccggatct ttcccaattg acggcagaag cggcacgctg gcggctgagc
     1741 gcccgatttc accattatcc cggttcactg ggagtacccg accgctagta cttttggact
     1801 tctgcgccgg cgcttcggag tgggccttgg gtactgtccg aaatccaact gtctttctat
     1861 tgtcatgttc ctatgagtcc ggcatagtga tgccatgaag ctgacggagc gtcgcagact
     1921 agaccggctg ccggcatccg ggtcgggcac gttggagtgg atcgacccaa gcagacgccg
     1981 cagaacggcc acggtgactg tcaaggacat caccgccgac ggcgttcggc tggaagccga
     2041 ccagccgctc gagacttgga atggggcgag gctgattgga gagacgtggg aatgcttggg
     2101 atctattcgt tattgccgcc cggaaaatgg caggtttgtg gctggcatgc agttgacgcg
     2161 gctgccaatc cgccgcactg atagccgggg gcctcagctt gtaaaacaga aacagacgac
     2221 tctaacgagg cccgcacaga agcttcctgc cggcaggccg gcggccggaa aggaagaccc
     2281 tgtcggccct gcggccccga aagcggctgc aaagccagcc gatccgcgca accctcgccc
     2341 atcttcggcg ccgatttcag cgctgaaagg cgagatcaga aaaggcgaag cctttgtcag
     2401 cgccgacagt gacgtcatcc ccgatcggcg gcgcagcgaa cgaactccca ccggcggttg
     2461 ggcgacattg gattgcgggc cgcattgtgt gactgttcgc gtcacgaatc tttcggacga
     2521 aggcgctacg atcgagttgg ctggccccgt cgaggcgccc caaatggctc ggctgaccgg
     2581 agaggtgtgg cagtgcctcg gctggctccg ctattgcaag ccggcagggg agaaatacgt
     2641 ggcgggcttc cagttcacgc gcagctaccc caagaattcg tgcgattatt tcgattagat
     2701 gcggcctatt ccgcgaggcc tttgacttct gattcgtttg ccttagggga tccaaggcgg
     2761 ccgccccgtt ttagaaaaaa tccgaaccgt ccggatgaat caagtcttct ctgcgatttc
     2821 tttttgcgct tcggcgagaa attcatccaa ggccttagct cccaggtcgc catgcttgcg
     2881 atgccgcacg gcgacctgct gcttttcaac ctcgcgcgct ccaaccacga gcatataggg
     2941 ggttttttgc aactgggcct cgcggatctt ggcattgatc ttctcgctgc gcgcgtccac
     3001 gccgacacgc agcccggctg cctgcaagcg gctagcgacg ctgcgcgcgt aatcgagctg
     3061 cttgtctgta atgggtaaaa tcacggcttg caccggcgcc agccacagcg gaaacgcgcc
     3121 ggcgtagtgc tccagaagca cgccgaaaaa gcgttccacc gatccccata acgcccgatg
     3181 aaccatgagc ggctgatgcg ccttgccgtc ctcgccgatg tactcgaggc cgaaccgccg
     3241 cggcagggtg aaatcgaact gcacggtcga gagctgccaa agccgcccga tggcgtcaac
     3301 cagcttgaca tcgattttgg gcccatagaa cgccgcctcg tcgggaatga ttctggcttc
     3361 aacgccgagt tgttgcagcg cctcgagcag cgcggtctcg gcgagacccc actggtcggg
     3421 cgttccgtcg tatttcccgc tggccccttt atcccaagtg gaaagctcga ctttatattc
     3481 ctgaaagcca aatgtgcgca gcacctcgag agcgaaatcc aggcagccgg cgatttcatc
     3541 tttgatctgc ccgggcgtgc aaaatatatg cgcgtcgtcc tgggtgaagc cccgcacgcg
     3601 cataaggccg tgcatgaccc cggagcgctc gtaccggtaa accgtgccca gttcgccgag
     3661 ccgcactggc agatcccggt agctgcgcag ccggtctttg taaatcagga tgtgaaaagg
     3721 gcagttcatc ggcttgaggc ggtagcgggc atcgtccagt tccatagccg caaacatatt
     3781 ttcggcgtaa tagttgaggt gtccggagat tttccacagt tcctcgcgcg ccacgtgggg
     3841 cgtgaacacg agcccataac cgcgccggag atactgctcg cgcagccagt cctccatcac
     3901 cttgcggaca atgccgccct ttgggtgcca gaagatcaga cccggcccgg ccgcatcctg
     3961 aatgctgaac aaatcgagct ccggtcccag cttgcgatga tcccgtttct tggcttcctc
     4021 gatacgttgc aggtaggcgt ccagctcctg ctgcgtaaag aaagacgtgc cgtagatgcg
     4081 ctgcatccgc gcattgtgtt cgtcgccctt ccaataggct ccggcgatcg aaagcagctt
     4141 cagggctttg atcttgcccg tagatggaat gtgcggcccg cggcaaaaat cgatgaaatg
     4201 agggccggtg gtatagcagg acacgcgatc gccttccgct ttctcttcga tgagctcgca
     4261 tttcatctct tcgcccatct ctcggaattt tttcaggctt tcctcccgcg gaagccagac
     4321 ccgctcgaac ggaaggtccc gcgccaccaa ctcgcgcatg cgagcttcaa ttttggccaa
     4381 atcttccgga gtgaacggtg tttcccgctg gaaatcgtag aaaaagccct cttcggtggg
     4441 cggcccgatg cccagcttgg tttccggata tagctccagt acggccgccg ccagcacgtg
     4501 cgcgctcgag tggcgataca cctcgagggc ctcgggatct ttgctggtaa gaatacggag
     4561 cggcgtgtcc tccatcagcg ggcttttgag gtcaatcagc cgcccgtcga tctttgccac
     4621 cagggccgct tctgcaagcc gcgggcccaa ttcccgcgcg atatcgagag gagtgatgcc
     4681 tttcgggaag gatctctcgc ttccgtcagg aaactttacc cgaatggttt ccatgctttc
     4741 tggttccaga tgagcggcct gagagcttaa tcctttttgc tcgtctcgga ggctttggac
     4801 tcgctttgga ttgactccga tttcccggag ccgttcccgg aggatttcgt ctctgtcttt
     4861 tcttttttcg tctcacccga cgcgccgctc tttctggcgt agtcggtgat ataccacccg
     4921 ctgcctttaa attggatcgc cggagccgaa agcaggcgct tcacgcgccc tccgcaaccc
     4981 tggtgtctcg cgaccggcgg gtcggaaaac ctctgaagtt cctcaaagcg accgccgcat
     5041 ttttggcatt cgtattcgta tacaggcata aagctttggc cgaaatactt caaaccatta
     5101 tagcagtaag cgagatcgta tggattctaa ccaccggctc cgagtcttag cgcaaacagc
     5161 cagaagccgg cactctccag ctttccccat ccctggacgg cgcggcccgc ccggcttaca
     5221 attccaggga tttgagatac tgccggaact cggttcccag atcctcgcgg cgcaaggcaa
     5281 actcgaccgt cgctttcaga aagccaagcc ggtcgccggc atcgtatctc gtgccctcaa
     5341 accggtacgc gtagactggt tgccgatcca ggagctgccg gatgccgtcg gtgagctgga
     5401 tctcaccgat gctgccgggc ttggtggcag ccaaggtgga aaatatctcc ggcaccagga
     5461 cgtaccgccc gatgatggca agatttgagg gcgccttttc cgcgggcggc ttttccacca
     5521 tatcccgcac gcgaaacagg cggtctcggg agccattgaa gggttcagca tcgcccgcga
     5581 tgactccgta ggccgaaatc ttttcccggg gcacttccat cacggccaaa acgcaacagc
     5641 ccgtgtgctc gaatacgtcc agcaattgcc gcaggcaggg ccgggcgctc tggatcacat
     5701 cgtccgcaag cacgacggcg aacggctcgt cgccggcgag ctcgcgagtc accagcacag
     5761 catgtcccag gccgagagcc tccttttgcc ggatgtaggc cacatgaatc aattcggaaa
     5821 cgtggcggac gatctccagt tccttgagct tgccgcgcga ttcaagcaga tattcgagct
     5881 cgaagctcac atcgaaatga tcttcgatgg ccgtcttgcc gcgcccggtg acgatgatga
     5941 tgttctcgat tcccgaatca attgcttctt ctaccccgta ctgaatgatg ggcttgtcca
     6001 ccagcggcag catctctttc ggctgcgatt tggtgacggg caggaatcgg gttcccaatc
     6061 cggcagcggg gaagacggct ttgcggacgc ggcagttcat gactccagta tatgcggacc
     6121 tggaaaaaga cttgccggga gagatccttt gttcgaaatg gctggtgtgc gggcgcccga
     6181 gctttccggg gtttgaaaag tatccctcag gctacaacac ggttgcggca aacgctctct
     6241 ctttctttga catgcccgtg gaactccttc gccagcggcc tatcggctcc aaatgcaagg
     6301 agaacttgat ccggcgtgcg cgtctacagc cgctggaacg agtcgcctcc catctccgcg
     6361 gcccgcacat tcctgggctg aacctatcat tttcaaggca agttcaatca ggcgatcgtg
     6421 gagtggggaa ggcttatttg cacccatcta gggatgatgc gccgggcgct ttccagggct
     6481 ggctggcggc ctcctggccg ggatcgatgt ccaggcttaa gccatctgct ttctggtagt
     6541 tggagaagga tgagggcgga acagtgtttg tagaaccaaa caaattctga tatattgttt
     6601 actcatgtct ttgagcagag gcgttggtgt ggccgtttgg gcagcagctc tggtttgggc
     6661 tgccgaggcc cctgaccctc ccaaggcggc ccctcaggta tatacggtgg gcgtggatgc
     6721 caggacggga aaactgattc gcatcccagc ccctaccaag agctctcctt ttgtgacaac
     6781 tcgccgggca gtgtcacgta aaagctcata tgaaacggcg gcagcgaacc cgccgggaac
     6841 ggaagtggaa aggggggcgg cgtcacagga ggctaccgca cttcagccgg acagggcatc
     6901 gagtcaagtc gagccaagcc cgcccggcaa gacgctggtc gtcatagatg aaccaaacca
     6961 gcgaacggaa gacgccgaaa aagggccaga agcggctggc ggccgaaggc cagcggccca
     7021 taacgttgtt cggcctgctg ctttcaacgg cctcgtggag cgcgtggcat tgcgctacga
     7081 cattaatccc ggcttcgtgc acgccgtcat caaagcggaa agcgactaca atccgcgcgc
     7141 tgtttcgcgg aagggcgccg tcgggctgat gcagctcacg ccgctgacag cgcggcggtt
     7201 tggcgtaagt aacctttata accctgtaga aaatctggag ggcggcgtcc gctatctgcg
     7261 gcatctgttg gatctataca attacgaccc caagctctcg ctcgccgcgt acaatgccgg
     7321 cgaaggcgcg gttgaccgct tccggggcgt cccgccctat tcggaaacac ggcggtacgt
     7381 gcggcgcgtg agcgagattt acagccggtc gttggtgtgg ctcgcgcccg cacctgcgcc
     7441 taagaagatc gaaggtcccc gcatttaccg cgttgtggaa ccttccggaa ccgtccggta
     7501 tgtgacggaa ccccaaccgg aatcgcagta gctgctatgc cgaaaacggc aaaccgacgc
     7561 cgggtgttcg ccctcgtgcc gggggttgtg gcgttgattg ccggctcccc gttcctgcac
     7621 ggcgccagcg cgaagcgcga actggcgctg caatattttc agcaagctca gcagcagcgg
     7681 ctcgatctcg aaagcgtccg ggaagccgat agaactcgag aaaattacca aaaggtgatt
     7741 ctggccttcc ggcgcgtcta cacgacttcg ccgcaaagct ccaaggcgga cgacagcttg
     7801 ctggcggtgg gcgacctcta ttcggaaatg agcgaacgct tcggaccggc gccctaccgc
     7861 gacaaggcca tccaggcgta ccgctttctc ataaatgaat atccgctgag cccccttctc
     7921 gaggatgccc gccagaagct gcaagcgctg gaaactcagg ccagcggagg tcgccctgcg
     7981 cccccagccg tccctcgcgc agtcgccgca tcccttgctt ccgccgggga agctgcgggc
     8041 gccggatccg gtttgcgcat cagtgacgtg cgctattttt catttcccga gctcactcgg
     8101 gtcgtaatca ccctcgatgc ggacgtggat gtgcacagcg agcgccttac ggatccggac
     8161 cgcgtttact tcgatcttcc caatacctgg ctcagcccgg cttggcaggg ccgaagcatc
     8221 catgtgggag accatctggt cagacgaatc cggattgccc acacgcgcgt cggggtaacg
     8281 cgcgtcgtgg tcgacttgaa tgccgatgcg cagtacgcca tctccaagct gaccaatccg
     8341 gcgcgcatcg tgctggaact ccgcaggcgc gacgccggta taaagccatt ccttgcgggc
     8401 gcggacgcag agcggcgaaa tctggaatcg gcgccgcaag gcccgctgag aaaggacggc
     8461 tcggctgcac agcccgccgc agcgaaaaca caggggcctt cggcagcatc tgcgattcga
     8521 gcatctgagc ctccgaggct tgccgaggtt ccgcaaaata cttctcgatc gccaaggtct
     8581 ctggacggca gagcggccga ctcaggtgac gcaagctcgc catcgccgcc gaaagaagct
     8641 gcgaaaaaag agccagcggc tgttccaggc aagaccacca ccgtatttaa ctccggggat
     8701 tttgctgaca gcagacggcc gcggccggga atcgactcgc caaagcccgc cggtgctgtg
     8761 gtggcgccga tagcggctgc ggcggccggc gttcccacac cgtctcctgc ggctttgcct
     8821 gctcagagtc ggccggtggc gccggcgctt ccggcggcgc cgctcgaatc ccgaccgaca
     8881 gttgagcaaa cctcggcggc ggctgttgac tcccgggcag ccccggcaaa gcccgctgct
     8941 tctccggccc tatcgaagct ggcgccgcca aagccggccc agcccaccag cagcggcgag
     9001 cgggacttga ttcgagctct cggcctgaag atttcccggg tggtcatcga cgccggccac
     9061 ggcgggcacg acaccggcac agtgggtccc acggggctca tggagaaaga tctgactctc
     9121 gatatcgcca cccggctcgg cgcgctgatc gctgaacgta tcggaagcga agtaatcttt
     9181 acccggactg atgatacatt tatcccgctg caagaccgca cggctctggc caatgagaaa
     9241 ggcgccgacc tgttcatctc cattcatgcc aattcctcgc gcgtgcgcgc cgtgcggggc
     9301 attgagacct actatctcaa cttgaccggc gaccgagagg ccctggagac cgcagcgcgg
     9361 gagaatgccg cctcccagaa atccattcac gagcttcagg acctggtttc gcagatcact
     9421 ctcacggaaa aaatctccga atcgcgcgag ttcgcctcgc aagtgcagcg atcgctatat
     9481 agcgcggtgt cgcgcgaaac cggagcgctc cggaaccggg gcgtacgtac tgcgcccttt
     9541 gtggtgttga tcggcgcccg catgccttcc gttctcgccg aagtcagttt tctcagcaat
     9601 ccgcgcgacg aaaagctgct caagaccggc gcctaccgcc agaaaatcgc cgaggccctc
     9661 tacaaaggac tggccagcta cgccggtacg ctgagccagg tggaagtggc gaaaaaatag
     9721 atcgccaaag ccggtcaatc ccccgcccgc tcgacctcga ccagcgtgct gtagaacgtg
     9781 ggtccgccgc ccatatcagt cagacgaccg gaaaccagcg cattgacgtt ttggccgtcc
     9841 ggcgaaagct tgttccagcg gcccgagagg ctcgcgatca cgccgcgcgg aacggtggcg
     9901 ctcactgcgg ccgccaaacg gcaactgccg cgctggttga agattcggac catatcgccg
     9961 tcggcgatgc cgcgaagagc agcgtcatcg gggtgaattt cgacgcaaaa ccccggctcg
    10021 gagccaggtt tgccatgacc gaatgtggta ttcacccgat ccggacattt gggtgaaatc
    10081 atttctaacg ggtatttccc ccgcagctcg gcatcgccga agcgcgattc caccggtggc
    10141 atgtaggaag gcagggggtc gatgccctgc ttgaccagcg agtcggctcg caattcacac
    10201 tttccggaag gcgtttcaaa ctccccttga gcaaacggta aaaacggctc gcccgcgggc
    10261 gcgaccggaa ggcgcacgga atgttcccga tccaattgct cgagggtgat tccccgcgcc
    10321 cagcgatgat ccgagctcag aagagcgcgc agcatctctt cctctgtctc gcgaaagcag
    10381 ggatcgtcaa agcccatgcg gcgcgccagg cggcggaaaa gctcgacgtt ggaaatggct
    10441 tctcccaagg gagcaatggc cggacgcgcc agttgcagat ggtagtgccc ataggcaaaa
    10501 tagagatctg tatgctcgag gaaagttgtg gccggcagga gaaaatcggc gtaatcggcg
    10561 gtatcggtct ggaactgctc gctgaccacg gtaaataaat cctcgcggcg cagcccccgc
    10621 agcacgcgag tctggtcggg agccacggcc gcgggattcg agttgtagac gaaaagcgct
    10681 tttaccggag gggagtccgc ctcgagcagc gctttgccca attcgaccat gttaattgtc
    10741 cggggcttcc ggccgagcgg cgagcgcagc cagagctcgg gcatttccag cctgtcgcgg
    10801 cgcagccgga aggcctgact ggtcgaaagc tgcaaaccgc cgccgatttc ccgccaggag
    10861 ccggtaatgg ccggcagcat ggcgatcgtc cgcacagcca tgccgccgcg ttcgctgcgc
    10921 tggagcccat aattcagccg gatcgccgcc ggccgtgtag tggcgtattc gcgcgccaaa
    10981 cggacgatgt cggccgcggc cacgccggtc agagccgccg ctttctgcgg cggatactcc
    11041 ttcacccgct cccgcaggtc ctcgaagccc agcgtgtagc gggccacgta atcggcgtcg
    11101 taaaggcctt caccgatgat cacgtgcagc acggccagcg ccagagccgt gtcgctgccc
    11161 ggataaacgg caatgtgcca gtcggcttgc cggctcgtac ggttctgatg cgggtcgatg
    11221 acgtaaaacc gcgccccggc gcggcgcgct tccacgatga acggccacaa gtggacgttg
    11281 gttgcaagaa tattggcgcc ccacgccaga atgagtttcg aatggcggaa ctgctccggc
    11341 tcggttccca cgcgcacgcc ttgcgtggcg gcgagaccgg ctgcgccggc ctccgaacag
    11401 atcgtccggt cgagcaagga cgcccccaag cgatggaaga agcggcggtc catgcccgcg
    11461 ccgttgagat atcccatggt cccgccatag ctgtacggca gaaccgactc cgggccgtac
    11521 gcggcgatta tggattgaag ccgtgcggcg atgctatcca gagcttcctc ccagccgatg
    11581 cgctcaaacc ggccttgacc cttcgcgccg acgcggcgca tgggatacag cacacgatcc
    11641 ggcgaataaa cccggtccag gtagcgcgct actttgccgc acaagaagcc ccgggtaacc
    11701 gggtgatcgg gatttccccg aatccgcact gcccttccct tgtcaacgtc aaccaggatc
    11761 gagcaggcgt cggggcagtc atgggaacaa acgctgtgaa gagtttcggg catgttgtct
    11821 ttgattataa gtgacaggtc ggcgaacgga aaccgcatct cgggactgtg cactgcttgc
    11881 ttcggggagc gcggcccccg catcggagcg gccggtgcgc agcgcgcgcg gagagcatcc
    11941 accggaagat tttggaaggc tgcttatata ttgatgatag gttctaagcg cctctccgct
    12001 gccggcgagg aagcggatac tgagagatcc gccaggcacg cggccgctct ctgccgctgg
    12061 aaagctgccg ccgcggcgct tttgctattc tatctcgcat gcgcagattg ttactgctat
    12121 cgattctctg cctggcgccc gcctgggccg cgggcgagct ttcgaaccgg cgcgcgccgg
    12181 ggttttcgct gccggattcc aacattcagc aacacgacat cgccgattat cgcggcaaaa
    12241 tcgttttgct cgagcttatg cagacctctt gcccgcactg cgtgaagttc gccggagtgc
    12301 tggaagaaat cagccgaaaa tacgcaggac gagtgcaagt gctttcgatt gtgacgcctc
    12361 cggatacaac ggccaccgta ggacccttca tccggcagca caacatcacc tacccgatcc
    12421 tgttcgactg cggccaggcc acgggttcgt acatgatggc gacgccgcag aagcccagct
    12481 ttgatattcc gcacgtgttc ttaatcgatc aggagggaat tatccgtaat gatttcggct
    12541 atggccctct gaccacggcg attttcgagg ggcgcggcct cttcgccgaa gtggataaga
    12601 tgctggcttc accgggcagc ggagcgccgc cgaaggcggc tccgaagaaa ccatagtagg
    12661 cgactttcag ctccactcgc agcttaagcc cgccgcgatc cttctcattc gcagccgcgg
    12721 aattgccggt acgcgtcgcg cgtcctctct gcggctcgcc gccaggtgaa ttgcgctgcg
    12781 cgggcgcgcc ctttttcgat caactcccgg cgcagcgagg aatcggcggc ggcccggctc
    12841 agtccttctg ccagcgcggc cgtgtccaac gggtcaacga gcagggcggc gccgccggca
    12901 atttcggcca aggccgagca attggaagtg accacaggaa ggccggcgga catggcctcc
    12961 aacaccggca atccgaaccc ttcttctagg gacggaaaag cgagagcggc ggccgagcga
    13021 taaagccgct cgatggcgcc gttgggcaga tagcccaggg cgcggatgcg ctgccgggcc
    13081 ggcgaattag cgatgcgcgc atgaatttct tccgccttgt atccgtccga gccggcaagc
    13141 accagatcga catcaccctc cagggtctca aacgcctcga tcaggcgaag gatgttctta
    13201 cgggcttgca gggcgccgac gtggagcagg aagggacgct cgagtcgaaa ccgccgccgg
    13261 aaggctttca gatcctcggc agcaaactcg ggaatgggat ctacaccgtg gtggatgacc
    13321 acgattcgcg aagactcgac gccgagcagc gcctccacac aatgcgctgt gtagcaagat
    13381 accgcaatta cgagcccgga gcgggccgcg gcgtctctgg ccagcgcggc gaatcgctcc
    13441 cgaaactcgg gcgtcgagta ttcgccggtg agcgcgaaca gatcgtggaa agtagtgatc
    13501 gcctggcgga agctatagcg cggcaagcgt tgattcaagc cgtgaaacac cgctacccgc
    13561 cggggcagaa agacattcag gggctcttcg agcagccggc gcgagacatt cgaaggccat
    13621 cgctgctcga gcaacgatcg aaaaaaccgg tgtggccggt aatacaggat aaaatggaac
    13681 tcgggagcag ccgccacgat ttcctcaatg agccgccggc agtacactcc tactccggag
    13741 agcgctcttc ccagactgta ggtggcatcg agtcccacat tcacgaaaca gcctccaaga
    13801 ccacatccac gatgcgctca gggtgggcgg gggcttccgg gctcacaacg accgtcttca
    13861 cttcggctgg gccgcaaggc cgccagcgct ggagaatgcg cggggaccgg aagccggcca
    13921 taatatcgac tgtcttcttt ccgagggcgg ccgctatgtg ctggcaggcc gaatcgtacc
    13981 caatgtagag atcgctgagg cggatcaatg cggcgaaccc ggctaggctg cctcgccagg
    14041 cgatgatttg cgcaccggca gaaggcggaa gaggttcccg ttcttcgatc tcgacgattc
    14101 gtgttccact ctcgcgcaat cgagctagca ggctgcctgt ccgcgccgat tcttccgccc
    14161 cgtcgccccg gtcgagaaaa actcgccagc cccggccgag caactctctc agcaaaagat
    14221 actcaaaatt gccggggatc ctctttccgg gattgtcgcc aactccgaaa ttgatgctga
    14281 tccaccccct taggggagcg gaaccggagc cctcctccct gtgggcgggt gagcgcaaaa
    14341 cggcgcgcag agactcagcc aatcgcagcg ctgcggcagg agggcttacc caaggccggg
    14401 cgggcgcggt ccccggccca aaggcatcgt cgagccaggc ggacgttaaa tcggagagcg
    14461 cctcacgtcc cggcttggcg taggaccggc tttcaaaaaa gagatagccc gcatcgcctg
    14521 cggtcaaagg cagcattccc agttgagtca ggcgggaatc agggtcgata atcacatatc
    14581 gcccggcggg cagacctttg atctcctccc ccacaatagc aaccactgcg ggccaggcag
    14641 ccagccggtc gagcaaaccc ccgcctctta gatacgacgc gcgccgcgct cgcacgcgag
    14701 gttcgcccgc aaacaggaga gcggcccgat cgctgcctac caatactagg tcggcgttcg
    14761 gaaacacctg catcattttc cgcaagacca cgctggtaac cgctacgtcg gccccgagag
    14821 tcacccgcga gagaacaagc gccttttcga tagctccggc ctcgactgcc gctcttccaa
    14881 acctgcgcag cgataaagcg cggcggatca aatccccctc attcgagatg ccaaagcgag
    14941 tcagttgcgc ttcgagccac gcggcgttgg aaagacggcg gcagcattcg agcagccggg
    15001 ccataaaccg gatgtacaat tcgcaacacg ccgggtcgaa agcgtccgcc aggggttcga
    15061 ccacttgccg aaaaatggcg tctgtcccgg agctagccag gtgcgcatcg gccagcgtag
    15121 cgatctcggc cagcagcggc acggcaagat ccagttcggc cgctgacgcg aggcaggcct
    15181 gcgccgcgtg cgccgccagc cgcgctgttt cagccggcgt cagcaaatct tgatcgcgca
    15241 gtttctggaa ttcggcgcaa gccgcggcag ctttttgggg gtgcacattc acacggtaca
    15301 caaggcgcca ctacaaggca atgggagccg cggagagcac cgcgtgcggc gcagctttgc
    15361 tggaggtcct ggcgccgcaa tccgccggat ttcaaaggcc ggcgtcattg acctttgcat
    15421 cgggcgcctt tgtgtcaagg ccataaccct tcaacttgcg gtagagggtt gtcttaccga
    15481 tgcccagcag tttggctgct ttccggcggt cgccccgagt cactttcatg gcatgctcga
    15541 tggcaagccg ttccacattg gccagaggcg ctacttccgg caaggccacc agccgcggct
    15601 cagcctgcaa cgccccggcc gcattccgga tttgggtggg cagatcgccc aagtgcagct
    15661 cattacccga acacagagca accatccgcc ggatggcgtt ctcgagctgc cgcacattac
    15721 ccggccagtc gtactccagc atcgcgcgga ggcttaacgg cgtcatggtc ataggacgcg
    15781 gagcgtagcg ctggagaaag tagcggatga gaagagggat gtcctctctg cgttcccgga
    15841 gcggcggcaa ccgcagcgcc acgacattca gcctgtaata caaatcggcg cggaatttcc
    15901 cttcctcgac gagcttcccg aggtcgcggt tcgtggcagc gattacgcgc acgtcgattt
    15961 tctcagcgtg ctcggcgccc agcggccgga cctcctgcgt ttcgagcacc cgcagcagct
    16021 tcggctgcaa ttccaggggc atttcaccga tctcgtccag gaacaacgcg cccccggagg
    16081 cctcttcaat taacccgcgt cgggcggcag tggcgccggt gaagctgccc cgcagatggc
    16141 cgaaaagctc gctctccacg agggaggcgg gcagcgcggg gacattcacc gcgacaaaaa
    16201 cgccgcgagt ccccggagcg accgcgtgca gagcgcgcgc caccaattct tttcctgtgc
    16261 cgctctcccc ggttacaaga accggactgc ggttgttcgc cagcttgtaa atgattcgca
    16321 gaacgcgttg gacttccggc gagtcaccga ccaaaccgaa agggtaagcc gcgctctcgg
    16381 acggcggttc ctgcatcaac cgagctatta tagcccggca ttggcgggga gctcatcgat
    16441 cctgatcacc agaggcgtga aattttcgcg ccgcagtttt tcaaccaggg cgttggcggc
    16501 gttccaatca gactcatgac ccaccagaac gcgatagaac agcccgcgcc cgctttcata
    16561 gctgcgaatg acagccgccc cgtgctcgcg agccatccgg gaacggaggc gctccgcatt
    16621 gccgcggctc tgaaaggaac ccacctggac ggcgtagaag ccgggagacg gcgtctcagg
    16681 caaaccgatg actgtcaacc ttacgcgcgc cgtaccgggc ccgatcatgg caatcctgcg
    16741 agccgcggca tgggagagat cgatgatgcg gtccttgtca aagggacccc gatcattgat
    16801 tcgcacttcc acagtccggc cattggataa attctcgacg cgcacccgcg caccgagagg
    16861 cagcgtgcgg tgcgccgcgg tcagcttttc catgtcgtag atttcaccgc tggtggtggg
    16921 acggccgtga taaggatgac cataccaact ggccataccc tcttccgtca tgccgatcga
    16981 aacgcgagga gccgcgggaa gggagacccc tgctttcctt cggccgcagc cggccggcgc
    17041 caggacagcc agcgagatag caaggagcgc gcccccgcgg ccgcggatca cttcctctct
    17101 tcctccgggg gctcgccatg gaggcggtcg gcccagcggc tcagttcttc ggaaagccgg
    17161 cgcagcaatt tctcgccgtc ccggcgagcg cggggcacta cgcgtccctc aataaaatcg
    17221 agcgcccgct tgacttcggc ttcgatttcc cgcgccgctt gctcgaaggc gtcagatcgg
    17281 ggggatttct cgcccatgcg ggtagtatac aacccgcgcc acggccgctg gtctgcgggc
    17341 atgaatacgg agcccgctct ggccggaagg aaacgaagca gcagcgcgcc ttatgtggga
    17401 atcaagccgc ggaattttcg cgcgtccgca gcgtaacccg gaaatacggc attaagattt
    17461 ggattgccca ggtgccgcga caccagctcg cccagcacgt cgcgaaaatc ggtggtgagg
    17521 ttcaggtcgc ggccttcgta aagctgctct ttttccattc ctggccactc gccgtagact
    17581 ttgccgccgc gaacatctcc gcccattaca aacattacgt tggcgtggcc atggtccgtg
    17641 ccccggttgc cgttctcgcg cgctgtacgc ccgaattcgg acatagtaac cagcgccacg
    17701 tccgccatac ggtctccgag atcctgccag aaggcggcca gggaacggcc aaactcccgg
    17761 agcagattcg gcaattggcc ggccgaggcc cgcgctccca cttcgttgac gtgatgatcc
    17821 cacccaccga tatcgacaaa ggctacctcg actccgacat cggctttaat gagccgggcg
    17881 atctgctgca agctctggcc gaaacggccc ccggggtagc tggccccgtt ttcagggctg
    17941 taaggacgct tctcgatcga agaaagcaga cggatcgcct cgaacgtctc ccggccggcg
    18001 ccccgcagca gcgtgtcgcc cgtcgtttga tacatcgtct cgaacggaac ggctgcgccg
    18061 ggatcgcgaa tctgaaagcc gggaagatca ttgatggcga gggccggagt ctcgccccgg
    18121 agcgcgcggg gcagggtcgg cccgacggcg acggcgcgga cgggagaaac cgtgccgcgc
    18181 tccggggcca gcgcccgatt catccaaccg ccaggcgtcg cctttcgccc gggcgttccg
    18241 gactccatgt agtcctgggc atcgaaatgg gaacgggtgg gatcgggcga acccactgca
    18301 tgaacaatcg ccaggtgggc gccccggtaa agcgggacga gaggcttcag cgcagggttc
    18361 aggccaaaga aaccgtcaag gtcgatggcc gagtcctcct ccgaaacgcc tggccgcggc
    18421 acggcaatcg tcgggcgcaa agagtaataa ctcttctcgc cgaacggaac gacgatgttc
    18481 agcccatcag ccgccccgcg ctgaaaaatt gcaaccagaa ccttcttgcg tctgccgcta
    18541 tatgtttctg cgaacaccgt gcgtgcgagc caggcggggg ctgagcctac accgaccata
    18601 gcgaacgcgg cgttctttaa aaatactctg cgcgtgtaca tataaagagc ctcacttgcg
    18661 ttgaaaatcg ggagaaccga tgacgagccc ggccacttgc agcggctgca agccgccctc
    18721 cgccgtatcg cttctggtta tggcctcgcg ggttgcgttg gaaggatcgg ccagcaggag
    18781 ctcccgcgcc acatccgccg gatcagccgc agctgtttgc gccacgcggt tcgggtccac
    18841 ccggatcccg ggcaggctgt ttcgggtcag ggcttcggcg aagttcatcc gggcgagaag
    18901 ggcggctgaa ttgatccaat cctggctcgc gttcgaataa cccgtcggtt cctgcttcct
    18961 gtagagcggt tcgccgagct gtgccgtctg gaagtccagc gcggcagcga aattgacctc
    19021 ggcatccagt ccccgcaccg cgctcacaac gatttcgagt ggtgacttga ccttggcccg
    19081 atacgctcct tgcgaccaaa actcgctgga agccagcatg gttttcatca cctcgcggat
    19141 gtcgccgccg ctcttcaagt aagttcgagc catcttttcc accagcgagt ccggcggatt
    19201 gtcagcaaca aaacgctgcg ccagcttgcg cgagatgaaa tgggccgttg aagggtggct
    19261 ggccagaatg tcaattacct tcaatccgtc ggaaagacca cccccggcag gaattttcac
    19321 ccccaacacg gtcttctcgc cccgatcgtg catcccctcg ttgaacacga acgttccgcc
    19381 gagctgcgga cggttgatcg tccagcctgt gaagcagcgc gctacttcaa tcacgtcccg
    19441 ctgggtgtag ccgccatcga ctcccattgt gtgcagttcc agcagttcac ggccgtaatt
    19501 ttcgtttagg ccccggcgag ggagggcctt ctggaaggtc aaaagccggc gccggccatt
    19561 taacatacgc tctgcggccg ccgggtccac cgattgccag ttatcgagat agaacagcat
    19621 ggccgggctc tgggccgtag ccagcagcag gtctttgaat ttcccgagaa cgtgaggccg
    19681 gatcgcatcc cgctcgtagg acgtcaccag atagcggtca gcccccttgt cgagaaacac
    19741 gttgaaatga ttgaaccaaa aatccacaag ctcttcttcg agttggcgat tgctgtatat
    19801 cgcacggtaa agtttgcttt cgaccaggtc gctgacgacc acctgctgcg gggcaaggga
    19861 gcggagcagc ctccgccgga gatcgtccgg cgcccgcgcg atgacagccc ggcgcatatt
    19921 cgccggtaaa acctcgatga gatcttcctg cttctcttct ggaagagaga aaaacaactt
    19981 cagcctttcc tccggcgtgc tttggcgaaa cgcgtttctt tcttccggcg acagcaggtt
    20041 tgcgagcggg gccatccccg ctattgccgg ttgccgccgg gcttccggct gctccgcttg
    20101 tccctgctgc tcaaaccggc gctcgtaacg ttcggtcagg cgttgaagcc gcttccgctt
    20161 ctccgggtcc tgcggatagg gaaggcgccc catcgccatg gcccggatca cttgtggggg
    20221 agggtagttc tccaccagct cgctcgtact catccgcagc gtatcgagca cgcgaagctt
    20281 ggcttcgagc gcggggtttt cggcgatgcg ctcggggtgg agctgtaaat caatccactt
    20341 cttcaatccc atggagcgaa cgcgttcgaa gtcgcccggc cgggggccga atgtcagccg
    20401 gttcagcgca tgagcgattt tctgctccga agaaagcttc ttggagaagg catcgtcttt
    20461 tttcgcgccc gggagtttgg ccggccccgc cgctaaaaaa agaagaaggc ccagtccaag
    20521 gaaggttggt ttgtagatcc tcatgccgcg atccctcacc cgatattgcg tcggcagccg
    20581 cgcagggcgt gacgcgctcc gcttcccggt cttctatctg tattaaacac ccgatggagg
    20641 ctgaaagttc tgcgtttcct caaccggcga gccgcgcatc agcgcggtga gactgtgccg
    20701 gatcgaccga tgttcccagg cgcctcaaag ccgctttgcc tttttccgaa gatcgcggca
    20761 agtgcgaagg ccctgcgtag cgagcctgga tgagcagacc agcgcagccc gcgcggcccc
    20821 agcccgattc attgccttac gcaagaacat ttcgattccc ggcgtttttt tcgttcgaag
    20881 actggcttca ccgctcgcgc tctcgttcga acggggcgtc ccaccggatg taagttcatt
    20941 tttttcttga ctttttttca cgatcccctt atacatggag acaacatcgt ggcgtatgcg
    21001 ccacaatttt tgatgttagg gagaatgttt cgatgaagaa agcagccaag aaaccggcaa
    21061 agaagaaagc cgcgaagaag aagaagtagc agtgcccgcc ccgtaaagag ggcgaaggcg
    21121 atttgcaaga aaggccccgt cccgattggg atggggcctt tttattttat attatggcgc
    21181 cggatgctca cgccgcgcgg ccaggcagca cgcgggtaat gcgagcaatg aaggaatact
    21241 tcagaggaag cggaatgtgc ggaaggacgc cgccgattcc cgcgccccgg cgtgctgcct
    21301 gcactcgagc cgccggcaat cgccgcttgc gctccccccc gctctctggc aggggcgtgt
    21361 cagccacccg acgaggtcgg gtttcgaaat cacccgggcg gaaaagcggg gccggttcct
    21421 cgcttttcgg gttctctttc actgaatcgg agagaaagca gcgggccgaa gcagagtgat
    21481 gttgctctgt gagacgatct gcccgccccg ggcaatcgac tcgtcgcgcg ccttgatcca
    21541 atccggcgat gccccgaagg cgtcccacgc tttttcgcgg tcggccagac tggcgaacgg
    21601 aatcagatag gtcaggttcg gcatgttctg gccgatcacg gtggtggtgt agagaatcgg
    21661 atggatgccc acggtatgga aaatcttgat ttcggggccg ccgaagcgct caagcaagag
    21721 cttcaactgc cgctgggtgg gcgagtgata gacacgcaat tcaaagacac gaggcgtctt
    21781 gggcttttct ttgagcggca caatttcggg agagaaatcc gtcgcttcca ggagcgcttg
    21841 atcggcgtaa tcgtacggcg gttcggaccc gttctccagt tcctcgtgcg ccttctgata
    21901 cccaggatcc tgagtgatcc gcttccgggc agcttccatt tcctcgtagc cggagaagcc
    21961 ggccaaaatc acagttgccg ggacgtgcgg cccgatgagg gagttgaaaa cgcccagcgt
    22021 ccgaatgttt ttcgtcaaca gaggcatttg cgacgagaga aaccggttga gacgtgtccc
    22081 ctggtctccc tgccggaagt agaagtattc gatccggtac agcctagtct ttcggccctg
    22141 cgcctgcgcc ggatcgctcg ctaacgcgga cagcatagtt gcgcctccca gagattgcac
    22201 gaaggaacgg cggttcataa tatctccctc ctgctgctgc gatttgttga aaccgtgttt
    22261 ataccacatc gagcgtctcc cgaaagggcc cttccgcatg ccgcacggcg gtgccggaac
    22321 tcggacggcc tcgattgccc gccccggcat cccagcccgg acgcaccccg cgcggtaaca
    22381 ggcgcctatt cccttcgtgg cacgcatcgg agggcgccag ttctcggatt agatcaagga
    22441 caatcgtagc tcgaaaggac gcagggggat cttctcaggg cgcgaactta acttttgtcc
    22501 tcgcctgacg atacattccc gatttgactt gcccgcatgc ctagcctggt cccttttctg
    22561 catgcatgtg ttaccatgcg cctaatggga gattccgcag ggcccgtagc tcagttggtt
    22621 agagcgctac cttgacacgg tagaggtctg gcgttcgagt cgccacgggc ccaccaccga
    22681 atgaaattct gatagttcgc tattggctcc cagcggggat tcagactcgc taggagcaga
    22741 tcacaggcgg aacgagcggg agcgcaggcg gcgagccgcc ccaccagctc caggtaccgg
    22801 cctcaggacg aacttctgaa ttccagagaa atcgccttcg attgcgaacc ccgcctgtgg
    22861 ccattcagga gcgttggcct gtgctgccgt cgcctggcca gcccaggcct gccgaagcat
    22921 cccgaagttc ggcatattgc aaccgaaaat atcaaagacg ccatagctcg cgcaaatgga
    22981 aaatgacatg taggctatgg tggttcgtgt caatcggcct gaatccaaag tgcattgagc
    23041 ggttaaggac cggctggtcc cagcagactg agatcttcgc ccagccgccc tgggagggac
    23101 gccctccgtg ttccctatcc ggttctcacc gcgaatctgg tctctcgctt aaagaagaaa
    23161 aactctcaag tgagaatttc gggtaaagga cccataccct ctgttcctac caagaccgga
    23221 tggccccttg cccgctcgat accaaagatt gtgggtcaat ttgaaatgga ccgcctgccg
    23281 gcccaaaatg ctccccttcc cggcggtgtt gattgtatgc taactgcggg aggtgcacca
    23341 tgaccacgct ccgggggccg gtcatttcca ttctggcggc tgggttgaca atcggcgccg
    23401 cgataccggc ggcgcagccg caagcgaccg ggcagagtgt cacgcagcaa gcgatcacgc
    23461 agcaatccgc atcccggcaa actacatctc agcagaccgt ctctcaacca accacttctc
    23521 aaccggtgat cacgccgacg gccaaagagc tcaaccagag cttcaatgcg gacgccgcgc
    23581 agtgggcggc gcgcttcgag cacgagggcc gcgccatcta cgacaagcgg ttcgagattt
    23641 tagacgccat gggactcaaa ccgggcatgg atgtcgccga catcggcgcc ggcagcggcc
    23701 tcatttcgcg gctgatcgcg cagagagtgg cgccgggcgg cactgtctac gccgtagata
    23761 tcgccaaaaa catggtcgat cacatcgcca ggaccgccca ggagcagaag atcactaacc
    23821 tgaaggctgt cctgggcgat cctcattcgc cgcaattacc acccaattcc gtcgatgctg
    23881 tatgtatcat tgatgcgtat caccatttcg agtacccgaa ggacatgctg gcggaaatca
    23941 acaaggcgct ccggccggac ggcctgctcc tgctgatcga cttcaagcgg attgaaggcg
    24001 tttcgcgccc gtacatcctg aaaatggtcc gggcggggga aggcactttc accgatgagt
    24061 ttttgaacgc cggctttgag ttgatcgagc gccgcgaaga catgttcccg gagaattacc
    24121 tgctcaaatt caggcatcgg cgcccccggg ccaactaaac agagcaacgc cgcacgcaag
    24181 catcgcaagc tctggcagcc gcgcggctga ggagccggcc gctgcctgga gtcttcccga
    24241 gcaaggccga ggcccgaacc gaaaagggca agccggcacg caggctataa agccgccggt
    24301 tgctggggcg ggtgtatact ggcactgatc cggatgggca gagcggaaga tccggcgggt
    24361 cggaggacga gacatgcggc cattgagatc tacggcgggc gatctatgca gaataccttc
    24421 tcgggcggtg caggcatcga tttttgcttt ccttgtcgga gtaaccggat ccccgtcagc
    24481 cgctcagcga tccgcttccc cggcgcgtgc gcaagctccc ttcaccgcgg ctgaatcgcg
    24541 cgcggttttt gacaaatatt gcgccgcatg ccataacgcg aagctcaaca ccgccgggct
    24601 cgcgctcgag tcgctggacg taaccaagcc gagcgccaat ccagagaagt gggagcgggt
    24661 gattcgcaag ctgcgcacgg gcacgatgcc gccgcggggc ctgccgcggc ccgatgaagc
    24721 gacctatgac gccgtagcaa gctggctcga gatggatatc gaccgcgctg cggcagccca
    24781 tccgaatccg ggccggacca actccgtgca ccgcctgaat cgcacggaat atcacaatgc
    24841 gattcgcgac ctcttcgcgc tcgactttga cgtaaattcg cagttgccgg gcgacgagac
    24901 ggccgacggc agtttcgaca acaacgccga cgtcctcggc attaccagat cccacctgga
    24961 acgctacatg tccgtggccc gccagatcac gcggctggcg acgggcctgc ctccggccag
    25021 cccggtggtg gaaacgttca aggttccgtt gcacgtggtc caggacgacc gccagagcga
    25081 agatctgccg ttcggatccc gcggcggcct ggccgttcgt tatgactttc cggccgacgg
    25141 cgactacacc ttcaaagttc gcctgcgcag gcagtatcag gactacatca tgggcatggg
    25201 gtggccgcag cgtctggaaa tccgcctgga cggcaagctc ctgaagcgct ttacaatcgg
    25261 cggccaggcg ccgggcaagc cctcacccgc cagtttcgcc ggagccggca actccttcgg
    25321 cgatcccgag tgggaaaaat acatgcaggg cgcggacgac ggattggaga tccgagtgcc
    25381 ggtcaaggcg ggaccgcggg ttgtcggcgt ggactttgtc cgggaactgt gggaacccga
    25441 actggttccg cagccgctgc aaagaggcag agtcatcacc gatgacgaga tttacatgga
    25501 ctacgcgagc gtggggtcgg tgcagatcgg cgggccctat caggtctccg gaccggcgcg
    25561 ggatacgccc agccgccgcg ccatttttgt atgccagcct cagacggctg cggaggagaa
    25621 gccctgcgcc gtaaggatcc tttcccggat ggcccggcgc gcttaccgcc ggccggtcac
    25681 cgatcgcgac gtgcaaacgc tgcttgagtt tttcgacagc ggccgccgcg acggcaagag
    25741 ctttgatgcc gggattcagt tcgcgctgga acgcctcctg gtcgatcccg atttcctgtt
    25801 gcgggtgctg cgcgatccgc ctcaggccaa gccgggagag gtctatcggc tgagcgatct
    25861 cgatctggcg tcccgcctgt cgttcttctt gtggagcagc atccccgatg agcagttgct
    25921 cgacttggcg gaaaagggaa agctctcgga tccgaaaatc ctggaaccgc aggtgcggcg
    25981 catgctggcc aattcgcgcg ccacagacgc cctggtcaac gacttcgccg cccagtggct
    26041 gaatctgcgc cgcgtcgggg atgtcgtggt cgatccccgc tactatccca attttgacga
    26101 gagcctgttg gaagccttca aagaggaaac gcaaatgttt gtcgccagca cgctccgcga
    26161 ggatcgcagc gtgttggacc tggtgaaagc ggattatact ttcgtcaacg aaaggctcgc
    26221 ccggcactac ggcattcccg gcatttacgg cagccgattc cggcgggtcc agctcccgaa
    26281 cctggaccag agaggcgggc tgctcgccga aggagcgctc ctcgcggcga cctcttaccc
    26341 cgaccggacc tcgcccgtac tgcgcggcaa atggctgctc gacaacatcc tcggcatgcc
    26401 tccaccgccg cctccgcccg gcgtgaacac cactttacag gaaccgaaac gcggggcgcg
    26461 gccggcctcc atacgcgagc ggctggacca gcaccgccat gaagccctct gcgccagttg
    26521 ccattcacaa atcgacccta tggggttcgc gctggagaac tttgacgttc tcggagggtg
    26581 gcggaccaag gatgaagcag gcaatcccat tgacagcacc ggcacgtggc cgagcggccg
    26641 gaagatcgat ggattcacgg ggctccgggc catgctgctc gaccacccgg acagcttcgt
    26701 cggcacattg acggaaaagc tgatgtccta cgccttggcg cgcaggctgg attactacga
    26761 caagccggct gtgcggaaga tcgtgcgcga tgccaaggcg aacgactatc gctggtcctc
    26821 cattattctg ggaattgtcc aaagcccgcc gtttcagatg cggtcggcca aagcggggac
    26881 ttaaaccccg ccgactcggg gcggtcttgg cacgctccgg cagcccgccg gcgcgagcgg
    26941 gttgtcaaaa atgtgtggag tcgtggaacc tgctaggatt ttcatcaacc ttcagagtaa
    27001 gatagggcga aggagagcac tgttatgact cggaaatcgc tgccgcggcg gaccgtattg
    27061 cggggtatgg gcatcagttt ggctctaccg ttgttggatt ccatgttccc tgcgttctcg
    27121 agagcgggga cgattgccaa accggtgcac cgattccagg cggtctatgt gccgaacggt
    27181 atggcgatgg aatattggtc gccgaagacg gtaggaaaag acttcgagct caccccaatt
    27241 ctggagccgc tggcgccgtt ccggaatcag atgatggtgc tctcgggcct ccatgccaat
    27301 tggaactaca ttcacgccgg cgcctcgggg tcgtttctga cagggactac gcgcggcggg
    27361 cggaccgagg tggagatcat cgcggatgtc tccatggatc agttactggc caagaaattc
    27421 gcgaacgaga cgcaattgcc ctctctcgag ctctcaatgg acgcccctaa taacgcggga
    27481 gcctgcacgg gagcgctgag ctgcgcctac acgcacacca tctcctggcg cagccccacg
    27541 cagccgttgc cgatggagca caatccgcgc gtggtgtttg agaagctgtt cggcgacacc
    27601 ggcagcacgg accgggcggc ccgcgaagcg cggctgcggc agcacaagag cattctggat
    27661 tcggtgaccg aaaagctcgc ggatctcaag cgggaggtcg ggccgcagga ccaggtaaag
    27721 gtggatgagt acaccgaagc ggtgcgcgac gtagagcggc gcgtgcagaa agccgagcag
    27781 cagaccaaca tcgaactgcc cgtcatggag cagcctcaag gcgtgcccac cgtcttcgaa
    27841 gaccacctgg atttgatgtt cgacttgcaa ctcttggcgc tgcggacaga tctgactcgc
    27901 gtgatcacgt tcatgatcgg taaagagcag agcgcccgtc cgtacccgca gattggggtt
    27961 cccgaagcgc accaccccct ctcgcaccac aataaccagc ccgacgtgat cgcgcgtatg
    28021 tcgaagatca accgctatca caccgagctg ttctcgaaat atctcgcgaa gctccgcgcg
    28081 acgccagacg gcgagggatc gctgttggac cacatgacga tcctttacgg ctcgggcatc
    28141 tccaacagca atcagcactc gggcgaaaac ctgcccattt tgctgctcgg cgggggcgcg
    28201 ggaaagctca aaggcgggcg gcacatcaaa tattccgacc ggccctcgat ggcaaacttg
    28261 ctcgtcacac tcatggagaa gctcgatgcg ccggtggagc gcatcggcgg cagcaccggc
    28321 aagctcccgc tcgatacgct ctctgacgtc tagcctggcg gcgctccagc gcgcaacggc
    28381 gagtacgatt ttcgcgtgcg tcttgcccag gcccacaact ggactgattt atacacaggg
    28441 agggaacacc gatgacagcc cgctggttac gcttgcttgc tctgggactg gccgtgaccc
    28501 tcgtggaggc cgccggctcc agcctggcgc tgattcaggc cgcccggaac accgacaaag
    28561 atgccttgca cgccctcctg ctgaagccgg gtgtggaggt caacgccgcc gagggcgacg
    28621 gctcgacggc tcttcattgg gcgagctacc gggacgatct ggaaggcgcg gatctgctga
    28681 ttcgcgccgg cgccaatgtc aatgccgcca acgatctcgg cgtcactccg ctctggatgg
    28741 cctgccagaa cggaagcgcc gccatggcgc gaaaactgct tgacgccggc gccgacccca
    28801 atgccaagct tctctctggt gagacgctgg taatgactgc ctcccgctcc gggaaggccg
    28861 aggtcgtcga actgctgctc gccaggggag ctgacgtgaa tgcgagggcc acgcgcggcc
    28921 aaacggcgtt aatgtgggcg gcggcccaaa agcatcccga agtcgtcaag gttttgctgg
    28981 cgcacggggc ggacgtcaaa gcccgctcgg atgtctggac ccaactatgg caaaccgcgc
    29041 ccgatcagga cgtccacccc gactaccagg tcaggctcaa actcgggggt gacacgcctc
    29101 tgctgtttgc ggcgcgagtt ggagatcttg cttccgcaaa gcttctcatc gaggccggcg
    29161 cggacgtaaa tgatgcctcg gcctacggaa ctagcgccac ggtgcttgcg gtgcatagcg
    29221 gcaatgccga gttggtggag tatctgctgg agaaaggcgc ggatcccaac gcggccggag
    29281 cgggctatac agcgctccac gccgcgattc tccgccgcaa tacgagggct gtggccgccc
    29341 tgctcgccca tggcgctgat ccgaacgcga agcttctggc ttccacgcct gtccggcgct
    29401 cctcgcatga tttctacttc catcccgcct atgtcggagc gacgccattc tggttggccg
    29461 cccgcttcac ccagcctgag gtcatgcgga tgctggccga gcacggcgcc gatcccctcg
    29521 tggtgcacaa tgtgagcttt tggggacgca gaaccaagac ctcggagtac aaacgggaag
    29581 cgccaggacc cacaaccgct ctgatggctg ccgttggcat gggccggggg agtgggtttg
    29641 cggctccaga accggcgcag cgggaagctc tgaccctgga ggcggtccag atcgcggtgg
    29701 agcttggcgt agacgtcaac gccgccgacg cagacggccg caccgctctg gaaaccgcga
    29761 cggccctcaa gtacaaatcg gtcgtcgagt ttctcacttc caagggcgct aaactagatc
    29821 atccggcacg gccccttccc aaggaagccg tcgataactg ataccatcgg cggactgcat
    29881 cgctttccag gacactatgc aacttgggcc tgcgtcgctc tgcgctttgg gcgcaacggc
    29941 aggtttctcc actggagtgt tcattgactt cagccgtcct tgttgcctaa acttagccaa
    30001 aagttcggcg ggagggcaat atgcttcctt tcggcgtagc tgtttcccgg agaaccattc
    30061 tgcgcgcagc cgccgcggcc gccgcttccg gagtcttgcg cccggcttcg ggccagcagc
    30121 gcggtccacg cgtgctggcg ttgatcggcg atcgctatca caacgcggat tacatccaag
    30181 tcgcgctgac caggatgttt gacggcctcg gcctggtcgt cgattacacc attgactaca
    30241 agcagctctc gcgaaaggtg cttcgcgact accagatatt tctctgcctg cgtgacggca
    30301 tgatatggcc gaacggctac ctggggcccg acgcctacac ttcttacgaa caagggctcg
    30361 agaaccgggg agactttccg gaagcgaaat cccaaagctg gctgacggaa gaacaggccg
    30421 tggccctaaa ggacttcgtc acggaaggga aaggcttcta ctctctccac aacaacagcc
    30481 atgtttccct ttcttcccga acgtaccggg aggtgatggg aggggcgtat atcgggcatc
    30541 ctccgctgcg gccgttccag gtgcatgtag tgaactcgaa tcatccgatc acccaaggaa
    30601 ttcacgattt catggtcaac gacgagcagc actacgtcga atatgacaaa gaccccaaat
    30661 acgttctcct ggaagcggaa aacatcgatg ggttgacctt tcggaacctg ggggcgaaat
    30721 caattgctgg gtgggcttac gattacggcc gcggccgagt tgtattcact gccgtaggac
    30781 acaccatcca tgccttatgg gccccggaat atctcaagct gcaaagacgg gcggttcaat
    30841 ggctgttaaa ggagatttga tctgattgga gttcacgtaa cagggtccca gcctggcctt
    30901 ttcgatgatc cgagcgaaaa ctccgggcct gctgccgccg tcgttttcac gggagatctc
    30961 actaccgccc ggaaaccttc ctcttctggg gccggcctcc gccacggcta aagcgatact
    31021 cgtggcggtc catttggacg cgcgcgccac gaaaggcgac cccttccgct tgcagacgca
    31081 ggcgctgctc gattccggct tcgccagaca gaagaatttt cccgccggct ccgatcaccc
    31141 ggtgccacgg caaattggcg gagcgcgggg agccgcggag agcccaggcc acctggcgcg
    31201 ctgtgcctgg aaatcccgcc gccctggcca cggctccgta gctcgagacg cggcccgagg
    31261 gaatcagccg gacaatctcc caaactcgct tgtagtagcc ttgcggtcgg cgcctacccg
    31321 gcatggacgc cggtcatgcg cggctcaatc tcgtcgagca gccgctcgac atccgcggcg
    31381 gtgaaaggct tggcaagcac agcctggttg ggctcggaaa acatttcccg aatatctgta
    31441 ctgaaagtgt cggccgtgag aaacacgaag ctctggatcc ggctttgcag acgccggtag
    31501 agctcgatgc cgctgattcc ccgcatgcga acgtcgcaca gaaccaggtc gaaccgcgcg
    31561 cgttcggcga ggtccagagc ttcctcggcg ctcgaaacgg tgatcacgcg gtactggcgg
    31621 tcgctcagca ggctcaataa ggaatcctgc accttccgat cttcatcgat gaccagtgcg
    31681 gtcaaagtgg ccggcgggcg ggactcggtt ttgcgcgccg caggaacctg ggcaggcttt
    31741 ggcggcaggc tgtcagccag aggatattcg atttcaaacg tcgttgcgtg attcgggctg
    31801 ttgctgaccc ggatcgatcc gccgtggccc tcgatgagac tctggcacaa cgccaggcca
    31861 aggcccgcgg tttcgatcgg ctttctggcg tcaaaaaacg ggtggtagac gcggctttgc
    31921 aaatcggcgc tgaagagcgg tccggtgtcg ctgatgtgaa tcaagacccg cttggctgcg
    31981 atcgtggtgc gaacggtgat caccttctcg cgggaaccct ccagggcgcg ctcggcgttg
    32041 atcagcaggt tgagaaacac ctgctccaaa tgggattgat cgctggcgac aatcggcgga
    32101 gtggtcgaga gctgcacgtg aacgcgaatg ccctggctct cccatttctc cgcccgaatc
    32161 gccaccaccg atcgcagcac ggcattgata tccacgagca caggccctga cttctgcggc
    32221 cgggcgaagg aaatcagacg ctgcaagatg gtgttggcgt ggcgcgcctc tgcgtgaatg
    32281 gcgcgcaggt cctgggccag cggaccggac ccgtatcggg ccaaagccag ctcggatagt
    32341 ccgctgagcg aggcaagtgg attcttgagc tcgtgcgcca ctccggagat caactcgccg
    32401 accgcggcca ttttctccgt gcgatagagc tgctcccgca tggaacgctg ctcgcacagg
    32461 cgcatggcca gcgcccccag gttcgcgacg tgctgcgccg aggctttttg ttcctcggta
    32521 aaagaccgtt tgcgccgccg gtcgtcgatt tcaagaaccc cgaaggtctc gccttcggaa
    32581 atgaggggca cgaacaacac ggatttttgc cggagctgct ccaccaccga gtggtccaga
    32641 aacgggcagt tttccgcatc gggaacttcg agcagcgcct tgttgcggaa gcaagtcaca
    32701 ggcccggaaa caacatccat cgagcagccg gcggccggaa actgatcggt tcccgcaagc
    32761 acgtccagtt gcttggtgac gcggttttgc aggaacagat agccgtgcgt cgcatccagc
    32821 atcgcaggca gcgtccgcac gatgcagcgg tagatttcgg ccggctcagc ggcggcgatc
    32881 atgtcttccg ccacggcgta cagattgctt aattgacgct gctgcgcggc atttctccgg
    32941 cggaagcgcc aagtagaaaa tgccagcgtg gagccgctga gagccagcat caacagcaac
    33001 aacagcagaa tatgcccgtt catcgtttcc aaccgccgga acactccagg caagccatgt
    33061 ttttaagaat ttgggccatt tgacaaccga ctggagatgc catgaatagt accaggagat
    33121 ttttagtact aaaactaccc cgtcatcgta tcgcaaaaca cgattccaac gcaaggtata
    33181 cgaccgtcct tcacccagcg gcgatcattt cgataaaatt agaaatatgg aagcgatctt
    33241 ggtcgagcgg tttgccgcag gatttgccgc cttaggcaat cccgcacgac ttcggattgt
    33301 tcggctgctt cttgcggcgt accagtccgg cggaatgacc gcgggaaaaa tccaagccga
    33361 actgggcatt ccggcctcca cgctaaccca ccatctcgcc aagctggaga gcgcggggtt
    33421 gatccgatcc cagcaggacc ggcaatggat ctggtactca atcgacgcca aaggtttgcg
    33481 agaga
//
LOCUS       DASREK010000247        14414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1598351, whole genome shotgun sequence.
ACCESSION   DASREK010000247 DASREK010000000
VERSION     DASREK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598351"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFA54_13205"
     CDS             <1..498
                     /locus_tag="VFA54_13205"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZS51817.1"
                     /translation="PVVVYNNPLYTGNDLGPSLLAEILQLENVIGLKQSSSDLGQLVE
                     VIRRAGSRVSICTGIDSQFYASLCVGVKGVFSTAACVIPREMAAIYRAFCAGDHQAAL
                     RAHQRAQILNRYFEYDPGYVAPCKEALGMLGIPAGPVRKPLPELTAEERVGVRDALRE
                     LGYEV"
     gene            503..844
                     /locus_tag="VFA54_13210"
     CDS             503..844
                     /locus_tag="VFA54_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS51818.1"
                     /translation="MTATDTAHIPWQERFNEKLGRVLYRKDLYTDAETGMEIRLVRYP
                     AGAINPLHTHPCGHGMYVLEGTLVTQQGSFGPGSFLWFPEGETMEHGASAESDATVLF
                     ITNKPFRIDYK"
     gene            945..2219
                     /locus_tag="VFA54_13215"
     CDS             945..2219
                     /locus_tag="VFA54_13215"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS51819.1"
                     /translation="MPNQITRRDFIGRTAAAAAAPALLRSQGTHEKVEVGWIGVGIRG
                     SSLIDILMDVAPKDAHVKAVCDTFAGHLARGKDKVQSKQKDTPDTYEDFRKMLEDKSI
                     DAVFIMTPEHLHHDMVIAALQAGKNVYCEKPLTHTIEEGLDILRAVEKSGKKLQVGTQ
                     RRSSLLYQRAKELYDSGALGKVTFVRAFWYRNSLPSEPQWRYEIPPDANPENTDFNKF
                     LGRAPKRSFSLPRYFQWRLYWDYSGGIATDLLVHQTDAAHMVLGAHAPKSVIASGGLY
                     YWNQDDREVPDTFTAAYEYPNGFQINYSCMFANKHYGYGEQFFGSEATMEVMNLQDLY
                     VYNEDFGKETPDKIKSRPEIHLNATRDFGQANPTNDHIKNFIEAVKYNKPLHCDARTG
                     HEAAVTGHLATLSFRRDKKVFWDADAQKYRVS"
     gene            2229..3419
                     /locus_tag="VFA54_13220"
     CDS             2229..3419
                     /locus_tag="VFA54_13220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51820.1"
                     /translation="MKAAEFSKVRLILAAILAIYVYGSIALLLGTVLPALSTQFHLDN
                     ERASWLPLANAAGLIISSLTIGPIIDKKGKKTALVWGLAVIVAGLLGLAAAPNYTLAL
                     ISLLLLGLGGGMIVTGANALVSDLEADRRASTLNLLNLFFGLGGMTTPFVAANILKIT
                     SAAAICYFLAALTVVTLLIHIATAMPPPAGGAPSVGQGYGQIFSRPVFYILAIELFLY
                     VGVEVGTETWLVQYLIGKWGEDARRVATNILSFGFAAGLLVGRVAVARLFAAVAEWKV
                     TLGFGLAIAATTFGMLQVDSQWAVILAVFAAGVACAPMFPTILAMTGNAFTQNSAQAL
                     GTVITIGWFGFLVIPPLIGKLGGANLGRGMLLLPACAVVIALLNLGLRRAPGTPRHPA
                     LAVS"
     gene            3635..4144
                     /locus_tag="VFA54_13225"
     CDS             3635..4144
                     /locus_tag="VFA54_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS51821.1"
                     /translation="MAKKVDPLNRKNYNAVCAMLTVSDVKAAASFYQKAFGFSRRGIM
                     NGPDGKPIHAELTLRGATLMLSPAMGPNAKTPKALGGSPASLYVLVENADKVVAKAVK
                     LGAETRMPVADMFWGDRCGVVVDPEGYSWMIATHIADPTLQEMKKKMKEQMQAMQSQS
                     AASGASQGQ"
     gene            4141..5427
                     /gene="larA"
                     /locus_tag="VFA54_13230"
     CDS             4141..5427
                     /gene="larA"
                     /locus_tag="VFA54_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HZS51822.1"
                     /translation="MKIRVAFDRCGAELALPDSWRTTVLEARFAGAVDSADAALERAL
                     AEPIASPPLRELARGKRSAAISVCDITRPAPNRIVLPHVTRALEQGGVPREGIRILIA
                     TGLHRAATEAELREIVGPEMLTRYPVESHNARIEESHQYLGRTAGGTEVLIDRRFMEA
                     DLHITLGFIEPHLMLGFSGGRKLIAPGLAAEKTIKRLHSPLFMRDPRAVEGGFPENPL
                     HRELLEIARMARHDFIVDVALTRTRQIARVFAGEPVAAHAAGVDFVRESTLAPVEEPV
                     DAVVTTGGGYPLDLTYYQSIKGVTAASHILKPGGKMLLLGACREGIGSPEFTRVVRTF
                     PNPKACLDGLENQPVIIDQWQLEKLALVARRAEIFWCVPGVPAEDRRYLWGPSFNHPQ
                     QALDALAAALPPEASVAVIPEGPYVFSQVAQVAAAV"
     gene            5497..6717
                     /locus_tag="VFA54_13235"
     CDS             5497..6717
                     /locus_tag="VFA54_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HZS51823.1"
                     /translation="MKRRFLAGAFLAVPWWVLAQRAPKAFDYSKELASPDDGRFRPSP
                     LPGIEEAYLPPLFASSHAANLLLLKSGDLLCFWFSGSREGESDVAIVMARLPKGSAQW
                     SRPQLVDHRPGASHQNPVPFEAPDGVLWLFHTTQPAGEGQANARVLATKSRDGGKTWT
                     AAETLFDVPGAFVRQPLLLMPDGRWMLPMYFTPSRGITTGAETNYSEIKISADHGGRW
                     TSCAIPNSNGYVQPNVLRVAGGRYVAFFRSRFADYIYRSDSSDGCTWTAPRRTELPNN
                     NASIQVAQLRNGHLVMAFNNARSEASGGRPRTAPRKPLSVALSEDGGETWSFVRDIET
                     GALRPGERPAEGIPDDQPGREEFSYPSVAQTADGRICVAYTYRRYTIKTVRFAEDWIK
                     QGTTIGLFRPRSAK"
     gene            complement(6766..8052)
                     /gene="serS"
                     /locus_tag="VFA54_13240"
     CDS             complement(6766..8052)
                     /gene="serS"
                     /locus_tag="VFA54_13240"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZS51824.1"
                     /translation="MLDLAFLRNNLEAVEARLSRRGGSAGLERFRELDRERRAAITQV
                     ERLKALRNAGSEEVARARREGRDTTEQQARLRELGEQIRLLEEKSKEYDGQLQEILAT
                     IPNVPHDSVPAGSGSEQNVEIRRWGRPKLMDFTPKAHWDLGPALGILDFDRAAKITGA
                     RFAVYWGLGARLERALMNFMLDVHTGQHGYTEVLPPFIVNSASLFGTGQLPKFAEDLF
                     KLEQHDFWLVPTAEVPVTNLYRKETIPAERLPIKIASFTACFRSEAGSYGRDVRGIIR
                     QHQFQKVELVKFARPEQSYQELESLTADAEDILQRLGLPYRTVVLCTGDLGFSSAKTY
                     DIEVWLPGLNEFKEISSCSNFEAFQARRAEIRYKPDARGKSDFVHTLNGSGLAIGRTW
                     LAILENYQQADGTVVVPDALRPYLNAERITPAGGLG"
     gene            8094..8519
                     /locus_tag="VFA54_13245"
     CDS             8094..8519
                     /locus_tag="VFA54_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51825.1"
                     /translation="MRSMHLWLLTLALLAGPACSRRPAETEEAVKQAVEKYLAARPGL
                     NMQGMTVDVGGLRFHDDKAETDVVFRARNDSKAMMSIHYTLRRQGSDWEVIPNSAAHA
                     GARPPARSEGSPELPPGHPSVGSAENPVLPPGHPPVKSQ"
     gene            8516..9703
                     /locus_tag="VFA54_13250"
     CDS             8516..9703
                     /locus_tag="VFA54_13250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZS51826.1"
                     /translation="MKVAVIGGGPAGAFAAEKLASSGIPVTVIDEKLAWEKPCGGGVT
                     ARALDAYPFLAASRSPARFVERAVLVASTGARACLRLRRPLAIYSREVLNGLLLDRAR
                     RAGADVVQDRIVDAERRDEAWRLQGKRDQYTADFCVVATGARNPLRQMGTELRREDAY
                     VALGYYVPGQLEHIEIQFCKGLEGYIWVFPRTDHLSVGICGKIDSQGTPRLRRMVERY
                     MAEHGLPVEGSRFYCHLLPSLAPSSFERNRVAGIGWAAVGDAAGLADPITGEGIFFAL
                     RSGDLLAECVIQERIADYASRLRRQVMDDLQLGARLAGVFYRGRFFFDSVTTRMVQFC
                     RRSRRFEDLMQDLIAGRQTYLGLKSRLWRQLGVTLWEIAGSVRAGTSSAELHEPSKIE
                     SPV"
     gene            9672..10961
                     /gene="hemL"
                     /locus_tag="VFA54_13255"
     CDS             9672..10961
                     /gene="hemL"
                     /locus_tag="VFA54_13255"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZS51827.1"
                     /translation="MNLPRSKALFEKAQKLMPGGVNSPVRAFRSVGGDPIFVACGEGA
                     HLLDVDGNRFIDYVGSWGPLLLGHRRPEVIHALCQALEEGTSFGAPTEREVEFAEMIK
                     EAAPSIEMVRLVNSGTEATMSVIRLARGFTGRDLIVKFEGCYHGHVDSLLVKAGSGVA
                     TLCIPDSVGVPAAFSATTIALPFNSQEAVEEAFRRHGKKIAAIIVEPVAGNMGCVPPA
                     PGFLHLLRRITEEHGALLIFDEVMTGFRLAMGGAQQLYGIRPDLTTLGKVIGGGLPAA
                     AYGGRADIMSRVAPLGPVYQAGTLSGNPLAVAAGIATLRILRDDPGIYGRLERRAAQL
                     CEGVAEAARNLQKPLAVNRVGSMFTFFFAPGPVTDYESAKRSDTSAFARFFRAMLERN
                     VYLPPSQFEAAFLSDAHTDRDIAETIRAAGEAIAEAY"
     gene            11087..11881
                     /locus_tag="VFA54_13260"
     CDS             11087..11881
                     /locus_tag="VFA54_13260"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZS51828.1"
                     /translation="MSVRTRPESRRAALHPIDIPANGRQEDEMIQVIRSGERHHEDMG
                     WLSTYWHFSFDTYYDPANMNWGPLRVFNDDMIQPGKGFGMHPHRDMEIISYVVAGALQ
                     HRDSQGNEGVVRAGEVQVMSAGSGIVHSEFNPAAEPVHLMQIWIQPRTKGLRPRWEQK
                     RFSRVERSGKLLPVVSFGDVEGTLAIDQDCVVYLSSLRKGQQVSHVAKSNRKGYLFVI
                     DGAVNLNSVPLATGDQARIDQETKLAVETRTGAELIFLDLPATSGG"
     gene            11997..12794
                     /locus_tag="VFA54_13265"
     CDS             11997..12794
                     /locus_tag="VFA54_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS51829.1"
                     /translation="MTCGTQFAATEQPPAACPICEDERQYVGWNGQQWTTLEALRENH
                     RNIFRLEGPGVTGIGTEPGFAIGQRALLVQTTAGNVLWDCVSLIDGATIRAVRNLGGI
                     STIAISHPHFYSSMVEWSRVFGAPIYLHAVERPWVMRPDPAIVFWEGETYSIGEGVTL
                     VRCGGHFEGAQVLHWAAGAEGRGALFTSDIIRVCLDRRYVSFMYSYPNLIPLPARAIR
                     RIVKALEPLRYDRIYGGWWGHNISHNAPAAVARSVERYLKAIGAAGA"
     gene            12926..>14414
                     /gene="glgB"
                     /locus_tag="VFA54_13270"
     CDS             12926..>14414
                     /gene="glgB"
                     /locus_tag="VFA54_13270"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HZS51830.1"
                     /translation="MALPGDIAAIVGAYHGDPFRVLGPHLVEENGRRRLEIRAFLPEA
                     KEANVLLDGQIHPMRRIAEEGFFEAVVGRNEVVPYRLQLTEESGATREIEDPYRFPPW
                     LTDFELHLHAEGNYYQSYEKLGAHLREVNGIAGVNFAVWAPNAQRVSVIGDFNRWDGR
                     RHPMRQRPGGIWEIFLPNQGENSIYKYEVKSLYRGHQEQKADPYAFASEKPPKSASVV
                     CDIDRYRWDDQEWMRRRAATDWLRSPISVYEVHLGSWMRSVMTYGELADRLIPYVLDM
                     GYTHIELLPVMEHPFDASWGYQVTGYFAPTARYGSPLEFMEFVDRCHQAGLGVILDWV
                     PGHFPKDAHGLAYFDGTALYEHEDPRLGEHRDWGTLIFNYGRNEVRNFLLSNGLFWLK
                     KYHIDGLRVDAVASMLYLDYSRSPGDWIPNKYGGRENLEAIEFLQKFNELAHAEAPGA
                     ITVAEESTAWPGVSRPVYLGGLGFTFKWNMGWMHDMLLYFSRDPIH"
BASE COUNT         2771 a         4334 c         4513 g         2796 t
ORIGIN      
        1 cccgtggtgg tatacaacaa tcccctgtac accggaaatg atctggggcc gtccttgctg
       61 gcggagattt tgcagttgga aaacgtcatc ggattgaaac agagctcgag cgacctcggc
      121 cagcttgttg aggtgatccg gcgcgccggc agccgggttt ccatttgcac cggaattgac
      181 agccagtttt acgcctccct gtgcgtgggc gtgaaaggcg ttttctcgac tgcggcgtgc
      241 gtaattcccc gcgagatggc ggccatttac agagcgttct gcgccgggga ccaccaggcg
      301 gcgctgcgtg cccaccagcg ggcgcagatt ttgaaccgct attttgaata cgatcccggc
      361 tacgtggctc cgtgcaagga agcgctcggc atgctcggca ttcccgctgg cccggtccgc
      421 aagccgctgc cggaattgac cgcggaagaa cgcgtcggcg tccgcgatgc tttgcgcgag
      481 ctcggctacg aggtgtaaat acatgaccgc aacagacacg gcccacatcc cctggcagga
      541 gcgcttcaac gaaaagcttg gccgggtgct atatcgaaag gacctgtaca cggatgccga
      601 aacgggcatg gagatccgcc tggtccgcta cccggccggc gcgatcaatc ccctgcatac
      661 gcatccttgc ggccacggca tgtacgtcct ggaagggacg ctggtgacgc agcagggcag
      721 tttcgggccg ggctcgtttt tgtggttccc cgaaggcgaa actatggaac acggcgcttc
      781 tgccgagagc gacgccacgg tcctcttcat aaccaataag ccgtttcgga tcgattacaa
      841 gtagcggtcc gttttggggc gcccggagcc gcccccagcg taacaggaga tacggaaaag
      901 ccagccagtc tgctattctc agatgtaaaa aagaggagag tcccgtgccg aaccagatca
      961 cgcgccgcga ttttatcggc cgcacagccg cggccgccgc cgcacctgcc ctactgcgct
     1021 ctcagggaac gcatgagaag gtcgaggtcg gctggattgg agtgggcatc cggggttcgt
     1081 cgctgatcga cattctcatg gacgttgcgc cgaaagacgc ccatgtcaag gccgtttgcg
     1141 ataccttcgc cggccatctg gcgcgcggca aggataaggt gcagtccaaa cagaaggata
     1201 ctcctgatac ttatgaagac ttccgcaaaa tgctcgagga taagtcgatt gacgctgtct
     1261 ttatcatgac tcccgagcat cttcaccacg acatggtgat tgccgctttg caggctggca
     1321 aaaatgtgta ctgcgaaaag cctctgacgc acacgatcga agaaggcctc gacatcctgc
     1381 gcgccgtcga gaagtcaggc aagaagctgc aagtgggcac gcagcgccgg agttctctgc
     1441 tctaccagcg ggccaaggag ctttatgatt cgggcgctct tggcaaagtg acattcgtgc
     1501 gggctttctg gtaccgcaac agcctgccca gcgagccgca gtggcgatat gaaatcccgc
     1561 ccgacgcgaa tcccgaaaac acggacttca acaagttctt gggccgagcg cccaagcgaa
     1621 gcttcagcct gccgcgttat ttccagtggc ggctgtactg ggattactcc ggcggcatcg
     1681 cgaccgacct gttagtccac cagaccgacg ccgcccacat ggtattggga gcccacgccc
     1741 cgaaatcggt catcgcatcc ggcggcctgt attactggaa tcaggacgac cgcgaagttc
     1801 ccgatacctt caccgccgcc tacgagtatc cgaatggatt ccagatcaac tacagttgca
     1861 tgttcgccaa caagcactac gggtatggag agcagttttt cggcagtgaa gcgacaatgg
     1921 aggtcatgaa tctgcaagac ctctacgttt ataacgaaga tttcgggaaa gaaactcccg
     1981 acaaaatcaa atcgcggcct gaaattcacc tcaacgccac aagggatttc ggccaggcca
     2041 acccgaccaa cgaccatatt aagaatttca tcgaggcggt gaaatacaat aagccgctgc
     2101 attgcgacgc gcgcaccggg cacgaagcgg cggttacggg gcatctggcg accctctcct
     2161 tccggcgcga caaaaaggtt ttctgggacg ccgacgccca gaaataccgg gtctcctgag
     2221 gacccgcgat gaaagccgcg gaattttcga aggtacggct gatactggcc gccattctcg
     2281 ccatctacgt ttacggttcg atcgccctgc tgcttggcac cgtccttccc gcgctttcga
     2341 cccagtttca cctcgacaat gagagggcct cgtggctgcc gctggccaac gccgcgggct
     2401 tgatcatttc gtcgctgacc atcggcccga tcatcgacaa aaaagggaaa aagacggctc
     2461 ttgtctgggg cctggccgtc atcgtcgccg gattgctcgg actggcggcg gccccgaact
     2521 acacgctcgc gctgatctcg ctgctgctgc tcggtctggg cgggggcatg attgtaaccg
     2581 gcgcgaacgc gctggtaagc gatctcgagg cggaccggcg ggcctccacg ctcaatctgc
     2641 tcaatttgtt tttcggtctc ggcggcatga ctactccgtt cgtcgccgcc aatattctaa
     2701 aaatcaccag cgccgccgcg atctgctatt ttctcgcggc cctgaccgtt gtgacattgc
     2761 tgatacacat agccacggcc atgcctcccc cggcgggagg cgcgccatcg gtcgggcagg
     2821 gttacgggca aatcttttct cgtcccgtgt tttacattct cgcaatcgaa ctgtttctct
     2881 acgtgggagt ggaggtcggt acggaaacgt ggctggtgca gtatctgatc ggcaagtggg
     2941 gcgaggacgc gcggcgagtc gcgaccaaca tcctctcctt cggctttgcg gccgggctgc
     3001 tcgtgggccg ggtggccgtt gcgcgcctgt ttgccgcggt cgctgaatgg aaggtgaccc
     3061 tgggattcgg cctggccatt gcggcgacga ctttcggcat gctgcaagta gactcgcaat
     3121 gggccgtcat cctggcggtc ttcgccgccg gcgtggcctg cgcgcctatg ttcccgacga
     3181 ttctcgccat gacgggcaac gccttcacgc agaactccgc gcaagccttg ggaacggtaa
     3241 ttaccatcgg atggttcggt tttctcgtaa ttccaccgct gatcggcaag ctcggagggg
     3301 ccaatctcgg gcggggtatg ctcctgctgc ccgcctgcgc cgtagtgatt gccctgctga
     3361 atctgggact gcgccgcgcc ccgggaacgc cgcggcatcc ggcattggcg gtttcgtaat
     3421 tcaaatcagg cggccctgtg gaaaagtccg cccgtggccc ctccaccgtt tcggtcaccc
     3481 gccgccgcaa caaccgaaca agcgcagaaa aaggaagcca tgcgcgagag gtacgaaccc
     3541 ggaagagtga aacctgccag gggcaagaaa agaaggggaa tgctaaaata aggcctgttt
     3601 ctgaggctgc tcatcaatca aggaggccgc cctcatggcc aagaaagtcg atcctctcaa
     3661 cagaaagaac tacaatgcgg tctgcgctat gctcaccgtg tctgacgtaa aggcggcggc
     3721 gagcttttac cagaaagcgt ttggcttcag caggcgcggc atcatgaatg gacccgacgg
     3781 caagcccatt cacgcggaac tgactctgcg gggagctact ctgatgctct cgccggcgat
     3841 gggtccgaac gccaagactc cgaaagccct cggcggctcg cctgccagct tatacgtgct
     3901 cgtggaaaat gcggataagg tggtcgccaa ggcggtgaaa ttaggcgccg agacgcgcat
     3961 gccggttgcc gacatgttct ggggcgaccg ttgtggagtt gtggtcgatc cagagggcta
     4021 ctcctggatg atcgcgaccc acattgcgga cccgacgctt caggaaatga agaagaaaat
     4081 gaaagagcag atgcaggcga tgcaatcaca atccgccgcc tcgggagctt cccagggaca
     4141 atgaaaattc gggtggcttt cgatcgttgc ggcgcggaac tggcgctgcc tgacagctgg
     4201 cgcaccacgg tgctcgaggc gcgcttcgcc ggcgcggtgg attcggccga cgccgcgctt
     4261 gagcgggctc tggcggaacc gatcgcttcc ccgccgctca gagagctggc gcggggcaag
     4321 cgcagcgcgg ctatttccgt ctgcgacatc acacgcccgg cgccaaacag gatcgtgctg
     4381 ccgcacgtca cgcgagccct cgagcagggg ggcgtgcccc gcgaaggcat ccggattttg
     4441 atcgccacag gattgcatcg cgcggcaacc gaggccgaat tgcgcgaaat cgtcgggccg
     4501 gagatgctga cgcggtatcc ggtggaatca cacaacgccc ggatcgaaga atcgcaccaa
     4561 tacctcggcc gcaccgccgg gggcaccgaa gtgctgatcg accggcgatt tatggaagcc
     4621 gacctgcaca tcaccttggg gttcatcgag ccgcacctga tgctgggctt ctcgggcggg
     4681 cgcaagctga tcgcccccgg ccttgcggcc gagaaaacga tcaagcgcct gcatagcccg
     4741 ctcttcatgc gggacccgcg cgcggtcgaa ggcggcttcc ctgagaaccc cctgcaccgc
     4801 gagcttctgg aaatcgcccg catggcccgg cacgatttca tcgtggacgt agccctgacg
     4861 cgcacccgcc agatcgcgcg cgtgtttgcc ggcgagccgg tcgctgcgca tgcggcgggc
     4921 gtcgatttcg tccgggaatc cactcttgcc ccggtggaag agcccgtcga cgcagtggtc
     4981 accaccggcg gcggataccc gcttgatctt acctattacc agagcataaa gggcgtcacg
     5041 gccgcttccc acattctgaa gcccggcggc aaaatgctgt tgcttggcgc ctgccgtgaa
     5101 ggcatcggca gcccggagtt cacgcgcgtc gtccgcacgt ttcccaatcc caaagcttgc
     5161 ctggatggtc tcgaaaacca gcccgtgatc attgaccaat ggcagctcga gaagctcgct
     5221 ctggtggccc gccgcgccga aattttctgg tgcgtgccgg gggtgccggc cgaggatcgg
     5281 cgatatcttt ggggaccgag tttcaaccac ccgcagcagg cgctcgatgc gctggcggcg
     5341 gccctgccgc cagaggcttc cgtcgccgtg atacccgaag gaccctacgt cttctcccag
     5401 gtcgcccagg ttgcggcagc ggtgtagcgg cttttccgtt cggttcctgt ctcaaggaaa
     5461 agattctgga ctgcgtagcc cggctgcgag gagaccatga aacggagatt tctggcaggc
     5521 gcttttctcg ccgttccctg gtgggtgttg gctcagaggg cgccgaaggc ttttgattat
     5581 tccaaagaac tggcctcgcc ggacgacggc cgcttccgcc cctctccctt gccaggaatc
     5641 gaggaggcct acctgcctcc gctcttcgcc tcatcgcacg ccgccaatct gcttttgctg
     5701 aagtccggcg atttgctttg cttttggttc tcagggtcgc gcgagggcga gtctgacgtc
     5761 gcgatcgtca tggcgcgcct gcccaagggg tctgctcaat ggagcaggcc acaactggtg
     5821 gaccaccgcc ccggtgcgtc acaccagaac cctgtgcctt tcgaggcgcc ggacggcgtt
     5881 ctatggctct ttcataccac acagcctgcg ggagaaggcc aggccaatgc tagggttctg
     5941 gcgaccaaat ccagagacgg cggcaaaacc tggacggccg ccgaaacgct gttcgatgtg
     6001 ccgggagcgt ttgtccgcca gccgctcctc ctgatgcctg acggaaggtg gatgctcccc
     6061 atgtatttca ccccgagcag aggcattacc acaggggcgg agacgaatta ttcggagatt
     6121 aagatttcag cggatcatgg cgggcgctgg acgagctgcg cgattccgaa ctcgaacggc
     6181 tacgtgcagc cgaatgtcct cagggtcgcc ggcgggcggt acgtggcttt tttccggagc
     6241 cgcttcgcag actacatata cagaagcgac tcaagcgacg gctgtacttg gacggccccg
     6301 cggcgcacgg agttgcccaa caataatgcg tccattcaag tggcgcagct tcgcaacggc
     6361 cacttggtga tggccttcaa caatgcgcgc tccgaagcca gcggcgggcg gccgcggacg
     6421 gcgccccgga agccgctctc cgtggccctt tccgaagatg gcggcgagac ctggagcttt
     6481 gtccgtgata tcgaaaccgg cgctttgagg ccgggtgagc ggccggcaga gggcattcca
     6541 gacgaccagc ccgggcggga ggaattttcc tatccctccg tggcgcaaac cgcggacggc
     6601 aggatctgcg tagcctatac ctatcgcaga tatacaatca agacggtccg gttcgccgaa
     6661 gactggatca agcaaggaac tacgattggg ttattccggc cccgttccgc aaagtagaac
     6721 cctgcggccg cccaccccgc gaccggccgg gcggcctcgc gggcctcacc cgaggccccc
     6781 tgccggtgta attcgctccg cgttcagata gggccgcaag gcgtcgggaa caaccaccgt
     6841 gccatccgct tgttgataat tttcgaggat ggccagccac gtgcgaccaa tggccaaacc
     6901 gctgccgttc aatgtgtgga cgaagtcgga cttgccgcga gcgtccggct tgtatcgaat
     6961 ctccgcccgg cgggcctgaa aagcttcaaa gttgctgcac gacgaaattt ccttgaactc
     7021 gttcaagccc ggaagccaga cttcgatgtc ataggttttg gcgctcgaga atccaaggtc
     7081 tcccgtgcat aacaccacgg tgcgatacgg caggccgaga cgctggagaa tatcctcggc
     7141 gtcggcggtc agcgactcga gctcctggta gctttgctcc gggcgtgcga atttcaccaa
     7201 ctcgactttt tggaactggt gctggcggat tatgccgcgg acgtcgcggc cgtaagaccc
     7261 tgcctcgctg cggaagcagg cggtgaatga ggcgattttg atcggcaggc gctcggccgg
     7321 gatggtctcc ttgcgataca ggttggtgac aggcacttca gcggtcggca cgagccagaa
     7381 gtcgtgctgt tccagcttga acaagtcttc ggcaaacttc gggagctggc cagtgccgaa
     7441 caaactggcg gaattgacta tgaatggcgg aagcacctcg gtgtagccgt gctggccggt
     7501 gtgcacgtca agcatgaaat tcatcagggc ccgctcgagc cgcgcgccca gcccccaata
     7561 gacggcgaag cgcgcgccgg tgattttggc ggcgcggtca aaatccagaa tgccgagcgc
     7621 cgggccgaga tcccagtggg cctttggagt gaaatccata agttttgggc ggccccagcg
     7681 gcggatttcg acattctgct cggagcccga tccggcgggc acggaatcgt ggggcacgtt
     7741 gggaatcgtg gcgagaattt cctggagctg gccgtcgtat tccttggatt tttcttcgag
     7801 cagcctgatc tgttcgccca gctcccgcag gcgggcctgc tgctccgtgg tatcgcggcc
     7861 ttcgcggcgg gcgcgcgcga cctcttcgct gccggcgttg cgcagagctt tgaggcgctc
     7921 tacctgggtg attgcggccc gccgctcgcg gtcgagttcg cgaaagcgct ccaggccggc
     7981 cgaacctccg cggcgcgaaa gacgcgcctc tacggcttcc aggttattgc gcagaaaagc
     8041 aaggtcgagc ataaaactca gtttatcgag aatcctgaga taatagggaa accatgagat
     8101 cgatgcacct atggctgctg acactggcgc tgcttgcggg tcccgcgtgc agccggcggc
     8161 cagcggaaac ggaagaagcc gtgaaacaag ccgttgaaaa atacctggct gcgcggcccg
     8221 gcttgaatat gcaaggcatg acggtcgatg tgggcggtct gcgctttcac gacgataagg
     8281 cagagaccga cgtagttttt cgcgctcgca acgattccaa ggcgatgatg tccattcact
     8341 acacgctccg ccggcagggc agcgactggg aagtgattcc caacagcgcc gcgcatgcgg
     8401 gagcgaggcc gccggcgcga tccgagggct ccccggaatt gcctccgggg cacccatccg
     8461 tcggttcggc cgaaaatccc gttctgccgc cgggacaccc gccggtcaaa tcccaatgaa
     8521 ggtcgcagtg ataggcggag gaccggcggg cgccttcgcc gccgagaagc tcgcctcgtc
     8581 gggcattccc gtaacagtca tcgatgagaa gttggcctgg gaaaagcctt gcgggggcgg
     8641 agtgaccgcc agagcgctcg acgcgtaccc ttttttggca gccagcagga gtccggctcg
     8701 gtttgtggag cgggcggtgc tcgtggcctc aaccggcgca cgcgcgtgcc tgcggctgcg
     8761 aaggccgctg gcgatctatt cgcgcgaggt gctcaacggc ctgcttctcg accgcgcgcg
     8821 gcgcgccggc gcggacgttg tgcaggatcg catcgtggac gccgagcgcc gggacgaggc
     8881 ctggcggctg caaggcaagc gggatcagta caccgctgat ttctgcgtcg tggcaacggg
     8941 ggcccgcaat ccgcttcggc agatgggcac ggaactccgc cgtgaggacg cctacgtcgc
     9001 gctcggctac tacgttcccg ggcagctcga acatatcgaa attcaatttt gcaagggtct
     9061 cgaaggctat atatgggtgt ttccgcgcac agaccatctg tcggtgggga tttgcggcaa
     9121 gatcgattcc caggggacgc cccgcttgcg gcgcatggtg gaacgctata tggccgagca
     9181 cggcttgccg gtcgagggat ctcgattcta ttgccatttg cttccctcgc tcgcgccttc
     9241 gagcttcgag cgcaaccgcg tggccggcat cggctgggcg gccgtgggcg atgcggctgg
     9301 gctcgccgac cccatcacgg gcgagggcat ttttttcgcg ctgcggtccg gcgacttgct
     9361 ggctgagtgc gtgatccagg aacgaatcgc cgactacgcc agccgtttgc ggcggcaggt
     9421 catggacgat ctgcaattgg gcgcccggct cgccggagtt ttttacagag gccggttttt
     9481 tttcgattcc gtcaccacgc gcatggtcca gttttgccgg cgcagccgaa gattcgaaga
     9541 tttgatgcaa gacctcatcg ccggcaggca gacctacctg gggctcaaga gccggctgtg
     9601 gcggcagttg ggagtcacct tgtgggagat cgccggcagc gtccgcgccg gaacctccag
     9661 cgctgagctt catgaacctt ccaagatcga aagccctgtt tgagaaagcg cagaagctga
     9721 tgccaggagg cgtcaactcg ccggtgcgtg cgttccgctc cgtaggggga gacccgattt
     9781 tcgtcgcctg cggcgaagga gcgcatctgc tagacgtcga cggcaatcgc ttcatcgact
     9841 atgtcggatc ctgggggccg ctgctgctgg ggcaccggcg tccggaagtg atacacgcgc
     9901 tctgtcaggc cctggaggag ggaaccagct tcggcgcgcc gaccgagcgg gaagtcgagt
     9961 ttgccgagat gatcaaagag gcggcgccgt cgatcgaaat ggtgcgtctg gtgaactcag
    10021 gaaccgaagc gaccatgagc gtcatccgcc tggcgcgtgg tttcaccggc cgggacctta
    10081 tcgtcaagtt tgagggatgc taccacgggc acgtggattc gttgctggtg aaggccggct
    10141 ctggcgtggc tacgctctgc atccccgatt ctgtcggtgt gccggcggcg ttctcggcca
    10201 cgacgattgc gttgccgttc aattcccagg aagccgtgga agaagcgttc cgccgccatg
    10261 ggaaaaaaat cgccgccatc attgttgaac ccgtcgccgg caacatgggc tgtgtgccgc
    10321 ccgctcccgg ttttctgcat ttgctccgcc gcatcacaga agagcacgga gcgttgctca
    10381 tcttcgacga agtgatgacc gggtttcgcc tggcgatggg cggcgcccag caactatacg
    10441 gaattcgccc cgatttaacc acgctcggca aggtgatcgg cggcggcctg ccggcggcgg
    10501 cctatggcgg ccgggccgac atcatgagca gggtggctcc gctcgggcct gtctaccagg
    10561 caggcaccct ttcgggcaac ccgctcgcgg tggccgccgg catcgcaacg ctgcgaatct
    10621 tgcgggatga tccgggcatc tacggccggc tcgagcggcg cgcggcgcag ctctgcgaag
    10681 gagtcgccga ggcggcccga aacttgcaaa agccgctggc cgtcaaccgg gttgggtcaa
    10741 tgttcacgtt ctttttcgcg ccaggccccg taaccgatta cgaatcggca aagcgaagcg
    10801 ataccagcgc gtttgcccgc tttttccggg ccatgctgga acggaacgtc tatttgccgc
    10861 cttcccagtt cgaggcggcc tttctttccg atgcccatac cgatcgcgat atcgccgaga
    10921 cgataagggc ggccggcgaa gccattgccg aagcatattg aagcatgttg acgagcgcgc
    10981 gtctgcacgg ccagtggcgc ggtgaattcc cctggcccaa tttcgacgaa actcgctgga
    11041 tcgttgcgcc cttgagaatc cagcctgtaa cgccgcgcag gatccgatga gcgtccggac
    11101 gcgtcctgaa tcccggcggg ccgcgctgca tccaatagac attcccgcaa atggacggca
    11161 agaggacgaa atgatccagg tgattcgatc cggcgagcgg catcacgaag acatgggttg
    11221 gctctccacc tattggcatt tttcgttcga tacttactac gatcccgcca atatgaattg
    11281 ggggcctctg cgagtgttca atgacgacat gattcaaccg ggcaagggct tcggcatgca
    11341 cccgcaccgc gatatggaga ttatcagcta cgtggttgca ggagctttac agcatcgcga
    11401 cagccaaggc aacgaaggcg tggttcgggc gggcgaggtg caggtgatgt cagcagggtc
    11461 cggcatcgtt cactcggagt tcaacccggc cgccgaaccg gtgcacctga tgcaaatctg
    11521 gattcagccg cgcacaaaag gacttaggcc gcgctgggag cagaaacgtt tttcccgtgt
    11581 agagcgctcc ggcaagctgc ttccggtggt ttctttcggg gacgtggagg gaaccctggc
    11641 tatcgaccag gattgtgttg tctacctctc cagcctgcgc aaggggcaac aggtaagcca
    11701 tgtggccaag tcgaaccgca agggctacct ttttgtgatt gacggggcgg tgaatctcaa
    11761 tagcgtcccg ctggcaacgg gcgatcaggc gcgcatcgac caggaaacca aactggctgt
    11821 tgagacaagg actggggctg agttgatctt tttggatttg ccggcaacca gcggcggatg
    11881 aggggcgtga gataggagcg gagcacggct gggtccacac gccggactcg ctggaagagc
    11941 cggtattcag cgcagcgctt gggaagaatc gggggttggc tatccactac atttgtgtga
    12001 cgtgcggcac ccaattcgct gccacggagc agccccctgc tgcctgcccg atctgcgaag
    12061 atgagcggca atatgtcgga tggaacgggc agcaatggac cacgttggag gccctgcgcg
    12121 agaatcacag gaacattttt agactggagg ggccgggcgt caccggcata ggcacggagc
    12181 cgggattcgc catcgggcag cgggcgttgc ttgtgcagac cacggcaggc aacgttttat
    12241 gggactgcgt cagcctgatt gatggcgcca ccattcgggc ggtacgcaac ctgggcggta
    12301 tttcaaccat cgccatctcc catccccatt tctattcctc gatggtcgaa tggagccggg
    12361 tcttcggcgc cccgatctat ttacacgcgg tcgagcggcc atgggtaatg cggcccgatc
    12421 cggctattgt gttttgggag ggagaaacgt attccatcgg tgaaggggtt acgctggtgc
    12481 gctgcggagg ccattttgaa ggcgcacagg tgctgcattg ggcggcggga gcggaaggcc
    12541 gaggcgcgct tttcaccagc gacattattc gcgtatgcct ggaccggcgc tatgtgagct
    12601 tcatgtatag ctatccaaac ctgatccctc tgcccgcccg cgccattcgc cgaatcgtca
    12661 aagcccttga gccgctccgg tacgaccgta tttacggcgg ctggtggggg cacaatatat
    12721 ctcacaacgc cccggcggcg gtggcccgct cagtggagcg ctatctgaaa gcgattggcg
    12781 cggccggcgc ctagacctgg ggcctcggcc aagctcccgg cgccattgaa tcggagcagc
    12841 ctccaacgga ggcccacaga ctcattaccg ttggtcctcc ggaggtcccg cggtcaagaa
    12901 acgggacttc agatatcatt tgtctatggc cttgccgggc gacatcgcgg ccattgtagg
    12961 cgcctaccac ggagaccctt tccgtgtgct agggccgcat ctggtcgaag agaacggccg
    13021 ccggcggctc gagatccgcg cctttttgcc cgaggcgaaa gaagcgaacg tgctgctgga
    13081 cgggcaaatc cacccgatgc ggcgcatcgc cgaggaagga tttttcgagg cggtcgtcgg
    13141 ccgcaatgaa gttgttcctt accggcttca gttaacagaa gaaagcggcg cgacgcgcga
    13201 aatcgaagac ccctaccggt ttcccccctg gttgaccgat ttcgaactcc atctgcatgc
    13261 ggaggggaat tattaccaga gctacgaaaa actgggtgcg cacctccgcg aagtgaatgg
    13321 aatagccggc gtaaacttcg ccgtctgggc tcccaacgcc cagcgggtca gcgtgatcgg
    13381 cgacttcaat cggtgggacg gccgccggca ccccatgcgc cagcggcccg gcggcatctg
    13441 ggaaattttt ctacccaatc agggcgagaa ctcgatttac aaatacgagg tcaaatcgct
    13501 ctatcgcggc catcaagaac aaaaagccga cccttacgca tttgcgtccg agaaacctcc
    13561 aaaatcggcg tccgtcgtct gtgacattga tcgctatcgg tgggacgatc aggaatggat
    13621 gcgccggcgc gccgccaccg actggctgcg atctcctatc tccgtttacg aggtgcatct
    13681 cggttcctgg atgcgctccg taatgaccta cggggaactg gcggaccggc tgattcccta
    13741 tgtgcttgat atgggatata cgcatatcga gctgctgccg gtgatggaac atccgtttga
    13801 tgcctcgtgg ggctatcagg taaccggcta ttttgctcct accgcccgat acggctcgcc
    13861 gctcgaattt atggagtttg tcgatcgctg tcatcaggcc ggcctggggg tgatcctcga
    13921 ttgggttccc ggccacttcc cgaaagacgc gcacggcctg gcttactttg acggcaccgc
    13981 gctttacgag cacgaagacc cgaggttggg ggaacaccgc gactggggca cgctgatttt
    14041 caattacggg cgcaacgaag tgcgaaattt cctgctatcg aacggcctgt tctggctgaa
    14101 aaaatatcac attgacggct tgcgtgtgga cgctgtggcc tcgatgcttt acctggatta
    14161 ctcgcgcagc cccggcgatt ggatccccaa taaatacggc gggcgggaaa acctggaagc
    14221 catcgagttc ttacagaagt tcaacgaact ggcccatgcg gaggcgccgg gagcaatcac
    14281 ggtggctgag gaatccaccg cctggcccgg agtttcgcgc ccggtctatc ttgggggcct
    14341 ggggttcacg ttcaagtgga acatgggatg gatgcacgat atgctcctct atttttcgcg
    14401 cgaccccatc cacc
//
LOCUS       DASREK010000248        58099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_451649, whole genome shotgun sequence.
ACCESSION   DASREK010000248 DASREK010000000
VERSION     DASREK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 58099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58099
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451649"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFA54_13275"
     CDS             <1..930
                     /locus_tag="VFA54_13275"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HZS51831.1"
                     /translation="TKEIDRRYQAIADFAEIGDFINRPVKTYSSGMIVRLAFAVAINV
                     EPEILLVDEALAVGDVYFRQRCMRKVHELRARQVTIVFVSHSMNDVKAIGDRVLWLEN
                     GQVVEIGPPESVVSKYLLHMMEKDTAYLKLRGSQSRPAGSTPVTAPEIVETIPNIDYR
                     HGNGAAAIIGIAVVDATGAPASLIEPGGQIIVRISARAKAPLDSPIVGFMMRNHLGVD
                     FSGTNTAREGHRLPPMAPGDVVTVDFHLTLPELYPGHFSFSPAIANGVLHAYEICDWI
                     DNAVTVQMGHSDGEIYGYVRFPCRVEVNGRLKN"
     gene            1087..3486
                     /locus_tag="VFA54_13280"
     CDS             1087..3486
                     /locus_tag="VFA54_13280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS51832.1"
                     /translation="MPRSIVISLRAAATAALLLLAAPPLLFILAICLLATDVASLLRG
                     KASLPPDIRPDTSAASVVIPNWNGRDLLEKYLPSVIAALEGNPANEIIVVDNASTDGS
                     AAFVRQRFPQVRVLEMEENLGFGGGSNAGFRAARNDIVVLLNSDMRVEPDFLAPLLEG
                     FYDEHVFAVSAQIFFADPRKKREETGLTESAWIDGRLHVRHIIDDRVRDLFPNFYAGG
                     GSSAYDRRKFLELCGFDPLYEPFYLEDTDLSYMAWKRGWTVHYQPRSVVHHEHRGTIG
                     KRFNDAYIGAVLKKNFLLFAWKNIHDWRLLLAHLANVYAGMWVRLFFGDTPGRTNLRS
                     LLRAARQLGQALAARRRARRLSVISDREAFRRPLGGYFRDRYEKPDPDRGRIRVLFVS
                     PYPIHPPVHGGAVFMSQTLAELSALAEVHLICLLDEKRDLVTNRLLDGCCASTEFLVR
                     MPPERNGWSPLLPHAARVFLSRDLEWRIHRTIFTQKIDVVQLEYTQLAVYAMEFKRLA
                     TFLFEHDVYFQSLARALRTERSPLRLLKTSFEYLRALRFELRAIEKFDAVQVCTRENK
                     TFLEPFLSSKRPLLDGLRAGIDVRRYRFVAEGRQPDTLLFVGNFQHLPNQQALAFFWA
                     RIWPLIQRERPQTRLIIVGAQAGPGFAEFYSAGGAEFLGYVEDIRQPLEQYAVFVCPV
                     LSGSGVRVKLLEAFATGIPAVSTPVGAEGLAYAGGEILELAATPEEFAAKTLALLADP
                     GRARAMARRARREVEQKWDIHALTQRLEAHYREVLRKKLAAAGVPLASVDLEQARSRA
                     I"
     gene            complement(3511..3993)
                     /locus_tag="VFA54_13285"
     CDS             complement(3511..3993)
                     /locus_tag="VFA54_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51833.1"
                     /translation="MKTLIVLILGVCLGASAAEAPAGLASVRKVYVTPMSAGLDQYLA
                     ETLASQGPFAVVVEPRQAEAVFSERVDPALAESLNLIYAPLSPPEKPKKDANTPQSDP
                     PPRRAPASARGNMFLIDVASRQVIWSTYLKLEDRSPHGLHRAAQDIVKRLKKDLSGKD
                     "
     gene            4054..4725
                     /locus_tag="VFA54_13290"
     CDS             4054..4725
                     /locus_tag="VFA54_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS51834.1"
                     /translation="MYKNHRITVIIPCLNEEQGIEQILRRMPEYVDEVIVVDNGSTDR
                     TAVVAQGLGATVIREDVRGYGRSYKKGFAHATGDLIVTLDGDHSYPVDGISYLLEAFL
                     HLQVDFLNASRFPVRDPRAMSFKHKLGNWALSLATSLLYFRWVRDSQSGMWVFRRSIL
                     PLMKLEADGMAFSEEIKIEAIKHPKIRFGEISILYSSRLGEMKLNPWKDGIDNLLFLI
                     KKRFV"
     gene            4847..5866
                     /locus_tag="VFA54_13295"
     CDS             4847..5866
                     /locus_tag="VFA54_13295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS51835.1"
                     /translation="MSPCVSFVIPNWNRRDLLLALLGSVRAQTLQPVEVLVVDNGSQD
                     GSVELAREAGARVLELKANRGFSFAVNRGIEAARGDLIAILNNDVELEPDWTEKLAAS
                     LEQSGGWFAIGKLLDYANRDRIDGAGDAICRGGAACRLGHARPDSVFFYTPRVTFFPS
                     ATAVLMRRGFLERAGNFDESFFAYLEDVDLGLRAALLDMGGIYTPGAVAYHRGSATLG
                     AWSSEMVKCLTRNQILLLAKFYPWALIWRFRIPILAAQALWAAMAARRGRLLAWLRGA
                     ASGLGSSLAVRRSAVRWRKDGNRLAEILLRSEGELVRVQSAAGWDDYWRWYFRLAPHP
                     QEPAP"
     gene            5863..6795
                     /locus_tag="VFA54_13300"
     CDS             5863..6795
                     /locus_tag="VFA54_13300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS51836.1"
                     /translation="MNLPVAAIVVTYNSELEIDNCLKSLENVAEIVVVDNGSADRTCE
                     VAAGRRIRVQLIANSENRGFAGAANQGAEATSSPLLLFLNPDAAVVSGLEALVRQFGD
                     PSVGAATGRLVDAAGRAQLGFNVRSFPTPASLACELLAVNRLWPGNPVNRRYRCLDMD
                     YHRPQNVEQPAGAFLMVRREVLRRVGGWDERFFPLWFEDVDLCRRIAGAGYSIWYVPG
                     CVAQHRGAHSLASVPAGQRRLYWYGSLLSYSNKHFSPSARAAIRLSVLLGASLRMAAS
                     LVRGREWQSYETVMRMALRRRKQETRQVQPHVLT"
     gene            6782..7831
                     /locus_tag="VFA54_13305"
     CDS             6782..7831
                     /locus_tag="VFA54_13305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS51837.1"
                     /translation="MYSRDLVSVTIVTYNSGRFIRRCLESLLDQKYPKLEVIIIDNAS
                     TDGTADILESFEDRCLVIYNDENLGFAAAQNQAIGLSNGNWVLTLNPDVFLLPGFIEA
                     LIEGGRLDPRAGTVCGKLFTISAGFDIPDKPVVDSTGIYFTPMLRHFDRGSQECDNGH
                     YLRYEYVFGATAAAACYRREMIEDVSINGEFFDPDFFSYREDADLAWRAQLLGWKCIY
                     VPYARAYHVRKLRPGMRRSVSPEINMHSVKNRFLMRLKNITPRLYLRHLLPITMRDLG
                     IIAYCLLSEQSSIKAFFITAQKLPKVLEKRRWIQARRRVDDAYIEQWFRSVPVSLPVE
                     GYAEPAEPKVSALHG"
     gene            7947..9050
                     /locus_tag="VFA54_13310"
     CDS             7947..9050
                     /locus_tag="VFA54_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HZS51838.1"
                     /translation="MRIALLGTRGIPGRYGGFETFAEELSIRLVRRNHQVTVYCRRNR
                     MSPGQSEYRGVRLVTLPTIAHKYLDTAAHTFFSTLHLLVRRADVALYCNGANTIFTIL
                     PRLAGVPALINVDGLERNRKKWNALARAWYAFSERLATLLPTAVITDAQSIADYYLAR
                     YNQPTYFIPYGAATGQVEGKETLDRLGIEPGKYWLYVSRMEPENNAHLVVRAFEKTSA
                     SMKLVMVGDAPYAGEYIRQVRATRDPRILFPGAIYGNGYHQLQAHCFAYIHATEVGGT
                     HPALVEAMGRGRPVLYLNNRENAEVAGGVGVPFDALESSLADAIERVAAMPGPERQKL
                     GELALQRVRERYDWESITDRYEQLLRKYAVSSG"
     gene            9166..10956
                     /locus_tag="VFA54_13315"
     CDS             9166..10956
                     /locus_tag="VFA54_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51839.1"
                     /translation="MRSGMRRPCFCLLFSFVVLPLAAQSSRPPVVLVNGYDVGWALRG
                     ACTPAAGSRDTFGSLEDFLKADGAPQVYFFDNCAQSGQQTLPNPSIEELGRRFGDFLA
                     NTVAAPQVDVVAHSMGGLIVRSYLSGKQPAFGSFMPPAGTKIRKIVFIATPHFGAPLA
                     GIVGSSPSDMQIREMAPGSAFLWDLATWNQGFDDLRGLDTLAVIGNGESDTKGDGVVS
                     LTSASLNSLLQPDQRTRIVPYCHISGLFFFGCPGPAIANADSSDQLTAQIVRSFLAGG
                     TAWTQIGETPSRNAVLSQQGGVLFGMKDASDSFQTPTQVSYKQSRDASISGQLSAGPS
                     FFYADLLAGGSYVFDAQAGAQSSDFLGTLPPGGFRVFLGKAGPVMKLIVPAAGVPDVL
                     ALAAGSIISIYGSNLAQGTKQASTQPLPAEMLGTSVLAQGRPLPLLYVSGQQINAVLP
                     AGLSGLTPLKLLTPGGQHTLNVWISPAAPAVFTLDSSGSGPGAVLHASTNLPVTASNP
                     AEPGEYVSIYATGLGPTMRSGDLDVAILTPQVLIGGAPATILFAGNAPGYLGLNQINV
                     QVPQIAAGPAVPLIVQSGGHSSNVVTLAIR"
     gene            complement(11057..11881)
                     /gene="larE"
                     /locus_tag="VFA54_13320"
     CDS             complement(11057..11881)
                     /gene="larE"
                     /locus_tag="VFA54_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZS51840.1"
                     /translation="MQPELAQKQERLFSILRGMGRVIVAFSGGTDSAYLAWAAHHVLG
                     DGALAVTADSASIPESHKRDAEEFVRRFGLRHEYIQTFEFENPDYVRNSPDRCFHCKD
                     ELFTRLEAIRRERGIPYVAYGVNVDDTGDFRPGQRAAKEHRVSAPLLEAGLTKAEIRE
                     LSRQMGLPTWDRPASACLSSRIPYGTPVTKENVKIVEKGEEAIKALGFRQFRVRFHGE
                     LVRIEVAPDELDRALNLEMARRLTFIFKNLGFQYVTLDLEGYRQGSLNEVLTVLKA"
     gene            12086..12241
                     /locus_tag="VFA54_13325"
     CDS             12086..12241
                     /locus_tag="VFA54_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51841.1"
                     /translation="MDEPPHISLRDEEEPPPVLGTWRRLYAAIALYLLLLIVLFYLFT
                     VTFNGAR"
     gene            12238..13917
                     /locus_tag="VFA54_13330"
     CDS             12238..13917
                     /locus_tag="VFA54_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HZS51842.1"
                     /translation="MRTLDWAVLVGSLVSIVLYGLYKGRGSSTVASYLLANKTMPWWA
                     MGLSIMATQTSAITFIGTTGQAYVDGMRFVQFYFGLPLAMIVVSAVSVPFFHRAGVYT
                     AYEFLEKRFDSKTRTLTSIVFLIQRGLSVGVSLSAPAVVMSVLLGWSDRKTILIMGGL
                     VIIYTVFGGIKAVTWTDVQQMTIMFLGLATALTMAIVSLPKGVSFRDALYLAGASGKL
                     NAVDLSFDWNTRYNLWSGLIGGMFLALSYFGCDQSQVQRYLTGKSIRHSRISLMFNAV
                     VKVPMQFFILLTGALVFVFFLFEQPPMLFNRAERERVESTDEYRAAEQRYEQTFAERR
                     RAAAALIASRDAAARRQYLSSLKDLETARAAGIDVVRRVTGTEYNDTNYIFLTFVTRY
                     LPEGLVGVVMAAVFAAAMGSIAGELNSLATASVMDIYRRHIKRQGGDAHYLVVSRVAT
                     GFWGLYAMIFAIFVTRLGSLIEAVNLVGSLFYGSVLGVFVLAFGVKRANGNGAVSGLL
                     AGLATVAWLSRNTGISFLWYNVAACVVVVVVGILVSGLRPASGGPGTVEVK"
     gene            13914..14879
                     /locus_tag="VFA54_13335"
     CDS             13914..14879
                     /locus_tag="VFA54_13335"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZS51843.1"
                     /translation="MTRLRCALLALLLLSASSGCRTQSRRVVGLAPKGVNHIFWQTVH
                     AGGIKAAQEFGFEIEWNAPALEIDSSRQIEIVESMINRRLAGIALAPVDKQALVGVVE
                     RAWREGIPVSIFDSDIDTDKRIAYVATDNLEGGRMAARRLGEVLEGKGKVGIVGFMAG
                     SASTMERERGFQEEMRARFPDIEIVGLQFGMADRAKAMAVTENMLTAHPDLAGLFADN
                     ESSSIGAVQALKSRGNKTVKLTAFDASDQLINDLRDGWIDSLVVQNPFRMGYEAVRAV
                     GLKLSGAEPPARVDSGATLVLRKDLERPEVKALLFPDIRAYLRAR"
     gene            15170..16126
                     /locus_tag="VFA54_13340"
     CDS             15170..16126
                     /locus_tag="VFA54_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HZS51844.1"
                     /translation="MRVRLFYHDRCFDGAASAAVFTEFFRNTYDRSAEFVCTGLAHKA
                     SQLFDEAQFDGDENAIVDFKYSSSPRVTWWFDHHQSAFLSPEDAEHFRHDKSGKKFYD
                     PTFKSCTKFLATIAREKFGFVQPRLDELIEWADIIDGAQYPDAKTAVELGSAAMKLTL
                     VIEGAGDSISQKMIRWMASRSLDDIANEPEVRALFNPLYQRHLESIDLIRQRADYQRG
                     VIFFDLVDCGHEGYNKFIPYYLFPDSLYTVSVNTSSFRTKVSVGSNPWAPREPGHNLA
                     TICERYGGGGHARVGAISFEPGALERARKAAAEIVEELRSES"
     gene            16132..16623
                     /locus_tag="VFA54_13345"
     CDS             16132..16623
                     /locus_tag="VFA54_13345"
                     /inference="COORDINATES: protein motif:HMM:NF040503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HZS51845.1"
                     /translation="MRARSATSDDAPAIARIYNEGIEDRTATFETRARSAGDVRTWFD
                     GLHPIIIVEERGRPIAFAATFSYRNRECYAGIAETSLYVARQWRGRGAGRLAMEALIE
                     AAEEAGFWKLLSRVFPENEASRALIRSLGFREVGIYHKHGKLDGIWRDVVIVERLIPK
                     NLK"
     gene            complement(16829..17947)
                     /locus_tag="VFA54_13350"
     CDS             complement(16829..17947)
                     /locus_tag="VFA54_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51846.1"
                     /translation="MCRFGLWLLALSCFVSGERLAVCETEGTVSETIRQHEYDLSTQG
                     RVFLLKEAGSASFFMLGELHGEKEIPALLRMIWPPMWQAGYRHIAAELSPWAANRLEF
                     RPPGASEPIRGLWSQAEATFAASFNRGRGAVLWGCDIEEAQPNLPIRDLAAANPGNRN
                     LQSALEMTRLGYRRSMARELLQRLREATGVKDLFAGGVSLRSNILRTLEIEIARLGAD
                     TRLEASIRREALMKELFYRHWQSSPQPKVLLRFGRNHLHRGYDRRGVSTLGNFVAELA
                     VARGLQAFNVAAFAAGGRIYWSGQLLEADERKDDPALEFLASLAQYPATVFDLRPIRQ
                     ALHRIPEIQRSLLEASLVYWADSYDAILCYREVTPIPH"
     gene            complement(18062..19198)
                     /gene="hpnI"
                     /locus_tag="VFA54_13355"
     CDS             complement(18062..19198)
                     /gene="hpnI"
                     /locus_tag="VFA54_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HZS51847.1"
                     /translation="MRLPELVLLALLAVCSLYHLAALLAALGRARPRPGRNARPFTPP
                     VSVLKPVRGLDPDFYAAIRSHAAQDYPEFELLFAVRDPADPAVGAIRRLAAEFPQRRI
                     DVFFTSRDYGANAKVNSLERLRAECRYDILVINDSDILAGPDYLRRVVAPLEKKETGL
                     VTCLYRGVPAGGLASVLEGLWISTDFQPGVLVARALGMRFALGATMALRRADLEQIGG
                     FAPLADYLADDYQLGKAITARGFDIALSDCVVETMLPRQPWAENWRHRLRWGRTLRVC
                     RPAGYAGMAITFAVPIVIAAVALQPSLWPEAMLCLGLRAAAAIAVGCMKLRDRAVVRY
                     FLLIPLADLASFAVWAASLFGRRVVWRSQSFRLQRGGRLAKESS"
     gene            complement(19195..19560)
                     /locus_tag="VFA54_13360"
     CDS             complement(19195..19560)
                     /locus_tag="VFA54_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZS51848.1"
                     /translation="MMIVFLLALVVLGTSLGDVFMTVGMKQHEIIDDFRPGPLGRSLA
                     VIIRNRWIILSIAAFAVSFFGFMGLVSVADLSFAVPATASAYVIETVLAVWILKETVS
                     RQRWLGAILVAIGVVLISL"
     gene            complement(19557..19988)
                     /locus_tag="VFA54_13365"
     CDS             complement(19557..19988)
                     /locus_tag="VFA54_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51849.1"
                     /translation="MKTRICLLIIVLSNALGNVVLGYGMRQIGSIASYSPLKLIVSSM
                     RALSNPFVLLGVALLVVFFIAHMIVLSWADLSYVLIMTSAGYIVVAALGWWLLDEPIK
                     PLRWIGILVITAGVVLVGGTPHSTLPKRSAEPERNQSQARL"
     gene            complement(20004..21482)
                     /gene="hpnJ"
                     /locus_tag="VFA54_13370"
     CDS             complement(20004..21482)
                     /gene="hpnJ"
                     /locus_tag="VFA54_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HZS51850.1"
                     /translation="MRTLFLNPPSFEGFDGGASSRWPATREIESYWYPVWLCYPAGML
                     PDSKVVDAPPHKISVRQTIEMAKDFEFLVLFTSTPGFHSDVRMAEMMQDANPRLKIAF
                     VGPHVSVLPEESLKASKAIHFVVRGEFDHPIVDFANGKPLEEIPGVSFRQNGGFRHNP
                     EGPKIEDLDSLPWVTKVYKRDFDITRYNVPFLLNPYLSFFTERGCPALCTFCLWPQTL
                     SGHPWRTRSANDVANEVRFALENFPGLKEIFFDDDTFNIRKSRVLELCEKLKPLKFTW
                     SCTSRVTVDYETLKAMKDAGCRLLIVGYESGDPQILKNIKKGATVERAIEFTRDCKKL
                     GLVIHGDFIIGLPGETRESINATIEFAKRLDTETIQVSIAHAYPGTEFYEYVKKNDLI
                     TIDSMTDDTGHQLPNVIYPGLDRGELVDAVERFYGEFYFRPKVVWRIVRKALFNGAER
                     RRLAKEAREYLALRAKRKKFVLDQRQVQPPQPRPIPAAPAGD"
     gene            complement(21524..22249)
                     /locus_tag="VFA54_13375"
     CDS             complement(21524..22249)
                     /locus_tag="VFA54_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51851.1"
                     /translation="MRSGASKSFLALALLFVGSLAAEPPHYDSVQILNSYLAVTRSQQ
                     DRLNGSTAEVSIDAEIPKLHKRGRLSALRRVSRLGRLTYDILRFEGDNTVKNDVIARY
                     LSTEVQVRDSHDPSLAINPENYKFSYKGVYAVEGRSVHVFALKPRHKRKGLFKGTLWI
                     DAQTFLPVRESGFLVKNPSVFIKKVYFTRDYQTTDGVAVVARLETTVETRVVGPAHMT
                     VQYADYRFEPSAMEVGLIPADSQ"
     gene            complement(22458..23096)
                     /locus_tag="VFA54_13380"
     CDS             complement(22458..23096)
                     /locus_tag="VFA54_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS51852.1"
                     /translation="MTKKIRILLADDHPVVRQGFRLILTAQPDMEIVGEAGNGREAVE
                     LAQGLLPDVVVMDVAMPELNGIEATRRLARVAPRTRVLALSMHKDSVYVREILRAGAR
                     GYLLKDSVDRDLLAAVRSVAGGEGYLSPGVADAVLSDYRRHVTDPLDLLSSREREVLQ
                     MIAEGKTNKEIAAALNLSVYTVDAHRGRIMEKLNLHSMGELVRFAMRSGLID"
     gene            complement(23093..24424)
                     /locus_tag="VFA54_13385"
     CDS             complement(23093..24424)
                     /locus_tag="VFA54_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZS51853.1"
                     /translation="MPAQPITSKSIVRVLVLGFALVILLLLAAGFVGVENARSIKENA
                     ANLVSQQLVTTRLIDEIQREEGNLTAVFNTLSRPAGAVDRERVLAQLNESDEHIEKVI
                     AAASGSDEDPLWQELKRASAGFTVEARRLLSAEDAHSFSSRELFRRHDEVIAAVAKLI
                     NASYQKASAAQDQIQRRSKELETKSLLLLGTCLVLALVCATLTMRMTAALFQKMEWQT
                     GELSRVSWHMLENQETTARRFSHELHDELGQSLAAVKANLAALAVDPSPAPERLADCA
                     HLVDEAIQNVRELSQLLRPIILDDFGLDAGLRWLGERFTERTGIAVEYQSNFAGRLAD
                     ETETHLFRIAQEALTNVARHSGATRVRLDLQNRDGAIQLSIADNGRGLSSESPRNGGL
                     GMIGMRARARDAGGEFSIRSGGGQGVVIKVEVPAKLADDGASVGREPALQS"
     gene            24707..25150
                     /locus_tag="VFA54_13390"
     CDS             24707..25150
                     /locus_tag="VFA54_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51854.1"
                     /translation="MNLSALLNLIWAAIAATALGCLAYAEFRSRNRRRSCVRRAVSVL
                     IAAFLLFPCVSASDDWLWVRALPLRPESGRGAGTSPINKSDEKASINLARLLEAVGNF
                     QVAGIYSLPFTACFFFLFRLPNRKLRTLRLSSRAGRDPPVFSLSR"
     gene            25184..26446
                     /locus_tag="VFA54_13395"
     CDS             25184..26446
                     /locus_tag="VFA54_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS51855.1"
                     /translation="MLRQYRRALACFLIPAGLSSICWAQKPLTWEEVRERFRMNNPAL
                     IAGRALIEESRASEITAYLRPNPEFTATVDQINPFTPNPTAYTPFAQTLPFVSTTYLH
                     ERQHKRELRRDSARLATQISTTDQADLERQLTFNLRDAFIRTLQAKSVLQLARENLGY
                     YDHVIDVNRERFKAGDISRADLMRVELQRVQFESDLVNAEVNLRTAKIALLALMNERL
                     PVDAFDITGDFDFHESSLRLEDARRAALEARPDLKSATTAIEKAQADNRLAWANGSAD
                     PTFGVDVARNPPIQAYVGFSVTVPLRIFDKNQGEKARTSIEIGRSERLREALLAGIYR
                     DVDSAYTSVESVRTLLRPYRDNYLPQATEIRDTVSFAYQNGGASLLEFLDAQKSYRDV
                     QLNYRNLIGSYLSAVNQLNLAVGREVIP"
     gene            26443..27591
                     /locus_tag="VFA54_13400"
     CDS             26443..27591
                     /locus_tag="VFA54_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS51856.1"
                     /translation="MKRRTQCLLGGAVLGLATLIFAACSRESSASASIPDTGPVKVEE
                     APDATLIQVDHPEQFPLVKVEARRTHDELVVNGVVAPDVSLTIPVNALAGGRVVEIRA
                     RLGDDVKAGQVLLRIHSPDLAAAIADYQKAKADESLARRSLERSQLLYSHGALALKDL
                     QQAQIAEDKAKVDLDTAAERIRILGGDVDHLSPIIELKAPASGTIIEQNTSSGAGVKS
                     LDNSPNLFTIANLSRVWVLCDVYENNLAQVRLGDFAEVRLNAYPDRPLRGKVSNISKM
                     LDPNTRTAKVRLELSNASGLLRPGMFATAKFISQGSRTRPVVASTAILRLHDKDWVFR
                     PAGERRFRRCEVQGGPVLPDGSQEILAGLQPGDEVVANALQFSSTVEQ"
     gene            27600..30737
                     /locus_tag="VFA54_13405"
     CDS             27600..30737
                     /locus_tag="VFA54_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZS51857.1"
                     /translation="MLRYLVDFALREKLLVLAVGGMLLIWGVISFHTLPIEAYPDVAN
                     TWVQVITQWPGHAAEEVEQQVTIPIETHTNGVLHLASMRSVSLFGLSVVTLVFDDEAD
                     NLLSRQQVLERLSQVNLPQNLTPQLGPDYSPVGQIYFYTLTSTNPRYDLMELKSLQDW
                     VLEKEFKSVPNVVDVSGFGGMVREYQVQVDPNKLISYGLSITQVEQALAANNTNAGGS
                     FIEHGQQAYNIRAIGLMTCTDDIGATLLKSQNGTPVRVRDVAVVTQGPKIRLGQIGKA
                     IHRQDGRVIDNDDVVEGIVLLRKGAEFDSTIEAIHEKVKDLNEHVLPPGVKIVPHLDR
                     GNLVHYTTQTVLHNLTEGILLVVAILFLFLGNIRSALIVTLTIPFSLLFASILLQLCH
                     IPANLLSLGALDFGMVVDGSVVMVENILRHVNRSPSELSFRDRIAIAAHEVQRPVFYA
                     RLIIITAYLPIFTLQRVEGRLFRPMAWTVAFALLGALIFALLLAPVLASYLFREGMRE
                     WRNPVLAWLTRAYRRRLDWCIHHRWITTGAAVAGLAAVLLLAYSGAVGSEFLPHLDEG
                     AIWARGTLPSSTGPTEGERVVRQARLIFAKFPEVTQVVSQLGRPDDGTDSTGFFNTEY
                     FVDLKPHEQWRKQFRSKEELIGAMDRELEKIPGVLWNFSQPIADNMEEAMSGVKGQLA
                     VKLYGSDLKQLEQKADEIVSVMKNIPGVADLGVFRVVGQPNVNLVVDRAKADRFGINV
                     ADIQDAVETAVGGKAVSQILQGERRYDLVVRYQEPYRRTVEDIAGIRIVAPSGERVSL
                     GQLCDIKIEDGASMIYREANSRYIAIKYSVRGRDLGGTVEAAMRAVAQKVKLPEGYRL
                     NWAGEYESQKRANRRLAIIVPITLLAIFLILYTAFDSFKWSLLILINVAMAPIGGILT
                     LYFTNTHFSVSSGLGFLALFGVSVEIGVIMVEYINQLRARGRSEREAAIEGAILRLRP
                     ILMTMLVATLGLLPAAISTGIGSDSQRPFARVIVGGLVADLLISVLLLPTLYVWMARP
                     DDKLPAAEMGAAGEE"
     gene            30981..32789
                     /locus_tag="VFA54_13410"
     CDS             30981..32789
                     /locus_tag="VFA54_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZS51858.1"
                     /translation="MPEPLLFALPGSPQTSRLRPFLLSMGIHAAVIGLAISSRGTQPS
                     TGAPPASAAQQTNSQRKIVWFTAPMRLPTVAPPEADEEQANRPNQTQTSQNIAATAKQ
                     PESRRQMVLQAPPELRLKSDVVSPNVLAFNPPAPSPPASKVRFVPPAKAAPVRLWALP
                     ELKEPQVTAEAHTPALPLQPTQVTPPRFQLPQRPAAAPKPSALAFSAPQIAVAAASDG
                     LLARLESKLSIERPKFVLPVRSAAEGRSGGRSPILAPAPQIALRPVQDQTLARLAPET
                     AASPPQFVMPKKKTAPVPSEALPLAGSPIPRVPVRAAIDNSLARIQPSTAPDMPKLAL
                     PQKPAAAGGAAGQILTPGPVPSVSAFPPAPATVIVGLDPAAAAPPPPPGNRSARFSAG
                     PDAAGREDSPTLSASAKAEIHVPNLSIRSGPDSPAAAGSGPPPAADRAAFRRQLMALA
                     TSTQPLSDRLRPSPDTAGPPILHGSTVYTMAIDMPNVTSYDGSWTLRFTELGGSSPDD
                     EITAPLAMHKVDPKYIASAAAEKIEGNVLLYAVIQRDGHVSQVRLVQGVDERLDSSAI
                     AAFSKWVFQPATKNGQPIDLEAVVQIPFRTGPQRRN"
     gene            33759..35129
                     /locus_tag="VFA54_13415"
     CDS             33759..35129
                     /locus_tag="VFA54_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS51859.1"
                     /translation="MIRLLLHSGDRKLQSLLAVTLGSDYDVRVEPDKSKVKEILSGEQ
                     ADVLILDFDSAQTSLDRLLAFLGEIRESRIPIVVMTDDEKRSTAMQLVQHGVYDLFRK
                     PPHLLELKLVVRRAHEHAQLKRELESAREKINSLSSCDQLIGSSAAMRSVYDLIRRVG
                     NLNTNVLIRGESGTGKELVARAIHNLSDRAKAPFIAVSCGAIPETLIEAELFGHDKGA
                     FTGATAAREGYFEQAGEGTLLLDEIGELSLQTQVKLLRVIQQREFCRVGGKKVLPLKA
                     RLVFATHRNLGKMVEEGTFRQDLYFRINVFKIELPPLKERKEDIPSLARHFLKQYCES
                     CGKRALELRPSAMQRLLDYEWPGNVRELENVIQRAVILAEDTSIGAEILPSAPPPAAT
                     PALATPIADSFEDQLKDYKVRLASQAVMECNGNKTLAARKLNITRAYLHRLLRQGAEN
                     DDVTVN"
     gene            35129..36646
                     /locus_tag="VFA54_13420"
     CDS             35129..36646
                     /locus_tag="VFA54_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51860.1"
                     /translation="MTAAEELGATLERPLAGRRDATAIRVRCLRAAFSFPAMLSVSFC
                     LLMLLTVRGRFDDPDMWWHLKVGEIIWNSHSIPRTDQFSYTTNQHSWLPHEWLSQLAM
                     YAAYRAGGYTGLWCCLFGLAALLLVSVYWLCSLYSGNSKLSMLGGLVAWFFGTVGLAV
                     RPLILGHLLLVVEVLILHLARSRGRAWLWALPPLFALWVNCHGSYALGIIVLGIAVTC
                     SFISLRAGRLFSIRREPQECRALCVVFLMCLAALMINPVGPKLLSYPLNTFTQQAEGL
                     AQVSEWQPTDIANPRGAGFFALAGAFALIFLLRPVQLELEGLLLLLVGFAMALQHVRL
                     LFAFGILAAPILCRLLADVWDHYDPDHDNRAANALLITAAIVVMICAFPNRSNLEQQV
                     RDHSPAAAVEFLRQTAISGPMLNEYEWGGYLIWALPEQKVFIDGRGDVFDWTGVFSEY
                     GRWATLQEDPEILLNKYRIHFCLLRAASPMARVLPYLPGWQNVYTDSQAVVFARK"
     gene            complement(37422..38510)
                     /locus_tag="VFA54_13425"
     CDS             complement(37422..38510)
                     /locus_tag="VFA54_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZS51861.1"
                     /translation="MRKTTLAVLLAAALAAPIFPQGDEKPASANDNPANTTDESTPKN
                     NKTKKKKVKEKSKDDVEAIGDRNVGSGVNFYSLEKEIALGKQLAQEVERQAKIIDDPI
                     IAEYINRVGQNLVRNSDAKVPFTIKVLDTDEPNAMSLPGGFFFVNSGLIQMADSESEM
                     AGAMAHEIAHVAARHGTRQATRGELVNLSTIPLIFMGGWTGYGIRQAAGLAIPLGFLQ
                     FSRGFEREADYLGVQYLYKTGYDPNGMVEMFERLEKLEKKKPGTLSKAFRTHPMTEDR
                     IEAAQKEIQEILPTKPEYVVNTSEFLDVKARLASLHARRKGEEQDSNRPRLRKSTSGS
                     TVPVEDKNGKSGTNDKSDDDDRPTLKRR"
     gene            38971..40098
                     /locus_tag="VFA54_13430"
     CDS             38971..40098
                     /locus_tag="VFA54_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZS51862.1"
                     /translation="MEFWEHYRREFPVTRQWIYMNHAAVSPLPRRAAQAMRGSIEDVS
                     LHGVVHHRRWTEACEALRSSAARLIGSSAEEIAIVKNTSEGLAIVANGIDWRPGDVVV
                     GVEGEFPANYYPWCNLQKRGVKLRWVQQRRGAVSLEDLDQACSGARLLAISFVQYLSG
                     FRADLEAIGEICARRECLFVVDAIQGLGAFPVDVRRARIHALCAGSHKWLLGPEGCGI
                     LYVNSEVIPQIEPVEFGWTNVAHWQDYESRDATLRAGAARYECGSLNTAGCYGLLAAL
                     DLLLEIGVERIGRRVAGLAQKIACGLSSAGYEILGRFEPASLSGIVSFKKPGADAARL
                     TQQLAAMRIIVAPRSGWVRASPHFYISDAEVEELVETLKTL"
     gene            complement(40176..40910)
                     /locus_tag="VFA54_13435"
     CDS             complement(40176..40910)
                     /locus_tag="VFA54_13435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51863.1"
                     /translation="MTLIELQNVGKRYFLHRKRQLLAERVASRIRRGRVSFWALRNVD
                     LRIEAGETVGIIGANGAGKSTLLSIAVGVSAPTEGVVIQRGRAAALLELGSGFHPDLT
                     GRENIHLNAALLGYTRAMVLKRIDPIIDFAGIADFIDEPLRTYSSGMMARLGFSVAIH
                     LDPDILVLDEVLAVGDQDFQRKCMERILEFRRQGKTLLFVSHSAQTIESLCRRAVWLE
                     MGRVKRDGPAAEVIAEYTRGVSALSV"
     gene            complement(40935..41702)
                     /locus_tag="VFA54_13440"
     CDS             complement(40935..41702)
                     /locus_tag="VFA54_13440"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS51864.1"
                     /translation="MTPAAHDHLYILYNLISKDFKIRYRNMSLGIFWSLLNPIIMVTV
                     LTYVFTKVFPQPIHNYPLFLLIGLVPFNFFTLAWATGTNSILDNAALVKKVRFHREVI
                     PISVVLGNVVHFLIQVVILLALLLLFGRGLNRHWLWLPVIFGLEIIFVCGMALAFSAL
                     DVHYRDTRYVVESANMVLFWMVPIFYDIGQVPVRYTLLYELNPVAAVVHACRLILLNA
                     TAPPFSLLEKLTLVSFAALAAGLLLFSRMKKEFADYL"
     gene            complement(41961..42242)
                     /locus_tag="VFA54_13445"
     CDS             complement(41961..42242)
                     /locus_tag="VFA54_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZS51865.1"
                     /translation="MDSPFPEPVRIPVADVFDLHTVPPREVRAAVEAYLEEAHRLGFR
                     CVRIIHGKGIGVQREMVRSVLARTPFVARFADAPEEAGGWGATIADLKP"
     gene            complement(42332..43345)
                     /locus_tag="VFA54_13450"
     CDS             complement(42332..43345)
                     /locus_tag="VFA54_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZS51866.1"
                     /translation="MAREKYSRQILFPPLGEQGQEKLFASRAVIVGCGALGSVQACAL
                     ARAGVGDIVIIDRDYIEPSNLQRQWLFDEADAARGLPKAVAAAEKLAAINSEVRVHPV
                     VADLTARNAEELLLPANVILDGADNFEARYLVNDVAVKHGVSWIYGAAVGSSGFTMPV
                     LPRVSACLSCLFPSPPSGNQPTCDTAGVLNAITALVACLQVADALKILSGSERSVEPR
                     LFSIDIWSNQWRSISTRERDPECLTCARSEFRYLNGPDRPPISLCGRNAVQIRAPAGP
                     VDLHSLGESLRRLGTVRANEHALRFLCPPYEMTVFPDGRAIVKGTSDISVARSLYTRY
                     VGG"
     gene            43494..46475
                     /locus_tag="VFA54_13455"
     CDS             43494..46475
                     /locus_tag="VFA54_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZS51867.1"
                     /translation="MFRSAFIAVALCLAPVWSVSGADNPFEGLPVTQILFIPAAQPIS
                     PARLKELIALREGAPLDGESLSKSIKQMFATGRYSDIQVDAERSANGVRISFITSQSW
                     FVGAVRVLNVEQPPTAAQLVAATNLVLGETYSDEKMKAATEALRALLAENGLYQAGIR
                     AETKGHPDTQQLDITFLVTPGPRAAFGDIRLPGRADVTAEQVRRITRWSKGRRFTQPA
                     VERGMARLQRYYQRTDHLMATARVAEREYVPGAGLVNLTIEIDPGPRVEVALSGAKLS
                     QKELRRLVPIYEEGSVDRDLLAEGARNLRDYFQTQGYFEAKVDYDEHPEENGVVLVEF
                     QATLGEPHEFVRLEIDGERYFDEETIRERMLLQPKGLHMRRGRFSQNLLSADIAAIQD
                     LYRSNGFLSAKVEPRVADDYQGRKGEIAVFLRIEEGPQTLVSRLTITGNQAISTRELL
                     NRASSLPGQPFSTTNVAGDRDQILDLYFGQGFADATLEWSSTPAAEPNRVNLEYTIHE
                     GRRQFVNKVIVEGYENTRPYIIDRQIQIENGQPLSQEAMLESQRRLYDLGIFSKVDMA
                     IQNPEGEEQYRNVLFETLEARRWTVGVGGGAEIGRIGGSTTSLDAPAGANGFSPRVSL
                     EVNRLNMFGRANTLSFQSRVSNFDKRVLARYTAPQWRGKERLTLTYNSLFDQSVDIRT
                     FSATRLEGGLQLQHRISKTTSLFYRYTYRHVTVDAATLKINPLLIPLFSQPVRVGLLS
                     ASLFQDRRDDPVDATRGMYNSIDLGSASYYTGSADTFARLLMQNSTYYPLTKRLVLAR
                     TTQFGAMVPYGRLRRVETSTESGAAQVTFTREIPLPERFFAGGPNSIRAFPINQAGPR
                     DLVTGFPVGGTGLFFNSVELRFPVRGPNLGGVLFHDMGNVYSKPQSISFRVTQRKPEF
                     DANGNIVSEDFDYMVHAVGLGVRYRTPIGPVRLDVAYGLNPPRFFGFKGSQEDLLFGR
                     GTKTNQRISHFQFHFSLGQTF"
     gene            46475..47146
                     /locus_tag="VFA54_13460"
     CDS             46475..47146
                     /locus_tag="VFA54_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51868.1"
                     /translation="MSARLLSRAISSFLLAAVSLPPSAARAEIIDRIVAVVGRQVVTD
                     SDVQREARLEAYFDGQPPPGSLAPQTPQYRQVLERLIRQQLIQYEMDQTRFPSVDAAQ
                     AKKQLQAMNPVQGKPGDYNLKEQDLIDYARRLANVARFLELRFGTLDAGWKPPAIPEE
                     QLETYYRDVFLPSLARKDNSSLPSLNDVRGEIEQTLRRETEIDAWLKELRSRVGVRIM
                     EPRKR"
     gene            47143..51108
                     /locus_tag="VFA54_13465"
     CDS             47143..51108
                     /locus_tag="VFA54_13465"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZS51869.1"
                     /translation="MKLAVRIGVALLALILALIGGGLLALRTGWFREKLRQRVVAELE
                     NDTGGRVEIARLDFDPQRWHARLEGLTIHGLEGLDQKPLFHARALEIDLKVVSFLEKR
                     VNIAGLRLEGPEIHIQVSPGGSTNIPAPRGSAAGRPVAEELLNLAVGRFELESGSLWW
                     NDRRYALEFAASGLRAQLRFEQAGRYIGQIEAREAQIALPPRIQEINHLAANFQISRD
                     QIEIGSVSGKTALSEFRAHGSITDFSQPHSQFDYDVAADAREAAAMLALPELKGGRVQ
                     LKGSVEARGARWSASGKLTGQQLAAAGREFNVRNVSLSGNYRLDPRGISVEPFAARLL
                     GGEWKGRIQADYKGQARVEGRAEGIRLEAAAAAVSSSSFPLSKLGWASVISGTIKARG
                     VWPSLFRSVNVRAELTLAAPADPGGRTPVQGTLRASYQAPTLDLQLDQMQLDMPASSL
                     TASGQLGRASSVQFRVATTRLEELTAAASAVFGEKVEPPVKLEGRAVFVGRASGAMRQ
                     PQIDAAVNVSRFVQEGREWDSFTGRVQWSRTRLRLTGGRLTRGATAVYANLMVGLEDG
                     RIEANSPVQGDFSIRDTQIQDLAALARQKTPFQGALTASLRVDGTRQNLRASGDIEIR
                     RGAAWGEPFGDFRAAVVLEPTQVQLSRFQVAKGSSAISGSGMFDHQRSLFRFDVRSEK
                     LALADWQVLEKTGKRVSGSGAFHLSGSGRLRAGETFPEDMALSGDLRLQKLSVDGRPF
                     GALAATVREQGNKLHVQAESDFTGAQIRMSADVVPRDRFPIDGRIEFRNANLSAAFQA
                     VRPVRLPEKVPADGDITISGEARSLASLVLAGTITRLEVPLPQTAAAARIQSLHSPEP
                     VKWRLAGRRLVVEAAHLVGEGTDLRFSGTASLDGPINFGAKGSVNLAVLPGLGPNVDV
                     RGASVIDAAVTGTLRQPDIRGSLEIRDASAGSENLPVSLSNANGTISFSRYSATIQKL
                     TAEAGGGQISFSGDATFLPAQPFTYRVSAEAHQVRLRYPKGLTTVLEAALTFAGTDQQ
                     SLLSGDIKVTRFGTRASMDVGALLVALRQPTRTPSRNGWLQGAQLNVAITTAPNIRFE
                     TSLARNFQADANLRLRGTVLNPALFGRINIIQGDFEFQGTRYAINRGEVTFSNPFRIE
                     PILNMDLETRVSSYDIVLTLSGPLQKLNVSYRSDPPLPFSEVITLLAVGRAPSTEGTL
                     AAQQNAQAQSLTQLGASTVIGQAIEKPVTGGLQRFFGVSRLKVDPELLGPEGNPNARV
                     TLEQQVSRDVTFTYIYNLASAQEQIVRIQWALSSQLSVIAVRDQNGVFGVDFLYRRRY
                     R"
     gene            51108..51767
                     /locus_tag="VFA54_13470"
     CDS             51108..51767
                     /locus_tag="VFA54_13470"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZS51870.1"
                     /translation="MPDWERLREEMVEKQLRRRGIRDFAVLEAMRRVPRHEFVPGPAA
                     TQAYSDEPLPIGAGQTISQPYMVAAMAEALHLNHCERVLEIGAGCGYHAAVLAELAAE
                     VFTIEREPELARLARENLSRLGYSRVVVIEGDGSLGYPDAAPYQAISVAAGAPGVPAR
                     LLEQLEENTGRLVIPVGSLDDQELRLIHKFTDGLQTRVVNYCRFVPLRGIMAWRLKGT
                     P"
     gene            51764..53710
                     /gene="selB"
                     /locus_tag="VFA54_13475"
     CDS             51764..53710
                     /gene="selB"
                     /locus_tag="VFA54_13475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZS51871.1"
                     /translation="MKHIIVGTAGHIDHGKTALVRALTGTDTDRLEEEKRRGISIDIG
                     FASLRLASGGEHHAAFDRGAKNGAEIELGFVDVPGHERFVKNMVAGIAGIDLVLFVVA
                     ADESIKPQTREHFDICRLLGIRHGIVVLTKTDLADRELIELVKLEVEEFTRGSFLEGA
                     PIVPVSSRTGEGIERLKSELHRMGEAVQPKDARHHFRMPIDRVFVIKGFGTVVTGTLI
                     SGTVEREAEVEIYPVGLRARVRGIEVHNQPVDRACAGQRTALNLAGVESSELARGMVV
                     AAAGRFRPTQRIDCVVDLLASARPLKHRAPVHFHTGTAEVEAEFYLFGKCAQLAPGVR
                     AFAQIRLGAPVLALPGDRFIIRQFSPVVTIGGGVVLDNLAAKHRSGEPVEDRLRLLVE
                     GGPCRTLEFILAEANSGLGLHEIITRTGWLESEVEAAAAEAVRVGLAERISEAPLWLV
                     HRDQFAAAAGRTVAMLERFHAENPLVPGPGKELLRASLFPGAPPAFLDALLERLQRDR
                     QVVIEGDVVRLRSHQIVLNEEESQAREKILAAFEQAGLTTPGVDDVLGKVPVERPRAQ
                     KILQMLLRERLLIRVAEGLVFHASAIESLKEMLARQKTKSDRLTVPQFKELTGISRKY
                     AIPLLEFLDREKITRRSGDERIIL"
     gene            complement(54004..56694)
                     /locus_tag="VFA54_13480"
     CDS             complement(54004..56694)
                     /locus_tag="VFA54_13480"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZS51872.1"
                     /translation="MWKGRLFASRPDAGAAGGKSWLRGPRGGKPDLAGCAMFTEAAAG
                     HRRSRWRSPAAVLGQGFAFWIAILLMLTAGAAPAQDDARAFRAARTGGNYMHNYYLPA
                     ASSTPWRPAWSPDGKEIAFAMSGSIWKIRVGDTTAYELTANRTYDSSPVWSPDGRWIA
                     YTAEDDGKTIHLMLLNVRTGESTVLTRGDSVNVDPAWSPDGKQLAYVSTAPNGWFNIY
                     VMPVNNGAPGAPVQITADHRFGRPRLYFSDYDSHIEPTWSPDGKEIIAISNRGIPLGS
                     GGIWRMPVKPDAMAGARLILREETLYRTRPQWSPDGKRILYSSHRGSQFTNLYVLPVE
                     GGEPYQLTFGAWDHFEPRWSPDGEWIVYVSNQNGLSELRLLKTFGGEERKIEIRRKVY
                     RRPMGTVEITVKDASSGQLAAARVYARAADGKTYAPPDAYQRVGERLGEHFFHTAGRS
                     VMQVPPGRLAIEAVKGFEYRPASQSIEVRPGEYRHVDLALSRMTNWKSQGWYSGSNHV
                     HMNYGGNLLNTPENMMLMASAEDIDVIEDKICNKDNRIFDYQYFTGSVDRRSTPQRLL
                     FFNEEYRPPFYGHVSFVNLAKHLISPFTTGYEGTAIESLYPSNTDIFQIAKAQGALTG
                     YVHPWTRDPEKAGYAASRGFPVDLALGVTDYLEVLTGAAYQKNTTAVWHRALNCGFRL
                     SGTGGEDSILSLHRTPIMGSDRTYAYLGPKLDYSAWVDAFREGRTFFTNGPLLDFHVN
                     GRMPGDEISLPAEGAVLQFSGKVESIVPLEKLEIINNGNVIQSMPLDGKGSAAEINTA
                     VRVSHSGWYTLRAHGGQPQRPIDDLFPYAETSPIYVICGGRPIRSAEDAQYFIRWIDD
                     ITRQAGQHSGWRSDKERQHVLAQFAEARRIFEQRRKEAAE"
     gene            56897..>58099
                     /locus_tag="VFA54_13485"
     CDS             56897..>58099
                     /locus_tag="VFA54_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HZS51873.1"
                     /translation="MQIIRSQEPYDVVVVGSGAGGGMAAKVLTDGGLKVAMLEAGGPL
                     NPEKDYYEHKWPYEFDHRGLRLPKGRSGFAAPNLFTEIDGEPYTVAPGEEFHWFRSRI
                     VGGRTNHWGRISLRYAPYDFKPYSTDGYGFDWPITYEDVAPYYDKVERYIGVFGTKEG
                     IPTAPDGIFQPPPKPRCTEVLIKRACDKLKILCIPSRLAILTRPLNGRPACHYCQQCG
                     RGCVTAANFSSSQVLIFPAQKSGRLTLITNAMAREVVTNREGKVTAVSYVDTNTRSEK
                     QVRCRAVVLAASACESARLLLNSKSSLFPNGLANGSGTVGRYLTDTVGAHVSGILPQL
                     AGLPSHNHDGVGGMHLYTPWWLYEKDRKPDFPRGYHIEYSGGPRMPAVGMFRGAAARL
                     EGYGADLKR"
BASE COUNT        11386 a        17550 c        17599 g        11564 t
ORIGIN      
        1 acgaaagaaa tcgaccggcg ctaccaggcg attgcggatt tcgctgaaat cggtgatttc
       61 atcaaccgcc cggtcaagac ttattcgagc ggaatgatcg tccggctggc cttcgctgtc
      121 gcgattaacg tggaacctga aatcctgctg gtggacgaag cgctcgccgt gggcgacgtg
      181 tatttccggc aacggtgcat gcgaaaagtg cacgagttgc gcgcgcgcca ggtgaccatc
      241 gtgtttgttt cgcacagcat gaatgacgta aaagccatcg gggaccgcgt gctgtggctg
      301 gagaacgggc aagtcgtcga gatcggccct ccggaatccg tggtgagcaa atatcttctg
      361 cacatgatgg agaaagacac ggcctacttg aagctgcgcg gttctcagtc gcgcccagcc
      421 ggatctactc cggtaacggc gcccgaaatc gtggaaacca ttcccaatat cgactacagg
      481 cacggcaatg gagcggcggc tatcatcggc atcgccgtgg tggatgcaac cggcgcgccc
      541 gccagcctga tcgagccggg aggccaaatc attgtgcgga tcagcgcccg cgccaaagct
      601 ccacttgatt cccctatcgt cggtttcatg atgcgcaacc atctgggcgt ggatttttcc
      661 ggaaccaaca ccgcccgcga gggccaccga ctgccgccga tggcccccgg cgatgttgtc
      721 acggtggatt ttcacctcac gctgccggag ttgtatcctg ggcatttctc cttctccccg
      781 gcgatcgcca acggcgtgtt gcatgcgtac gagatttgcg actggatcga taatgccgta
      841 acggtgcaga tgggacacag cgacggcgag atttacggct acgtccggtt tccctgccgc
      901 gtggaggtga acggaagatt gaagaattga gaagccggag aatgaataaa acaagaggga
      961 gaaaacgaca attcagaact ctggaaccgc caggccgtag gcggccgtca ggtgaagctt
     1021 gacggctcca ctccggccag gccggctggc ctcgcgactg cgaacctcgc attctgaatg
     1081 tctccgatgc cccgatcgat tgtgatctcg ctgcgcgcgg cggcgacggc ggcgttgctg
     1141 ctgcttgcag cgccgccatt gctgttcatt ctggcgatat gcctgctggc gaccgatgtg
     1201 gcttcgctgc tgcgaggcaa ggcgtcgctg ccgcccgata tacgcccgga taccagcgct
     1261 gcgtcggtgg tcattcccaa ctggaatgga cgggatctgc tggagaagta cctgccgtcg
     1321 gtgatcgccg ctctggaagg caacccggcc aatgaaataa tcgtcgtcga taacgcctca
     1381 accgacggca gcgcggcgtt tgtgcgccag cgctttccac aggtccgggt gctcgaaatg
     1441 gaggaaaacc tcggcttcgg cggcggctcg aacgccggct tccgggccgc ccgcaacgat
     1501 atcgtcgtgc tgctgaacag cgatatgcgg gtggagcccg atttcctcgc gcccctgctg
     1561 gagggcttct atgacgaaca tgttttcgcc gtctcggcgc agatattttt cgccgatccc
     1621 cgaaaaaaac gcgaggagac cggcttgacc gagagcgcgt ggatcgatgg ccgcctgcac
     1681 gtgcggcaca tcatcgacga ccgcgtacgg gacttgttcc cgaacttcta tgccggcggc
     1741 ggctcatccg cctatgaccg ccgcaaattt ctagagttgt gcggattcga tccgctctac
     1801 gagccttttt atctcgaaga taccgacctg agctacatgg cctggaagcg cggctggacg
     1861 gtgcactatc agccgcgcag cgtggtgcat cacgagcatc gtggaaccat cggcaagcgg
     1921 tttaacgacg cctatatcgg cgccgtactg aaaaaaaatt tcctgctttt tgcctggaaa
     1981 aacatccatg actggcgcct tttgctcgcc cacctcgcca acgtgtacgc gggcatgtgg
     2041 gtccggttgt ttttcggcga cacgccgggc cggacgaatt tacgctcgct gctgcgagct
     2101 gcgcggcagc ttggacaagc cttggcggcg cgcaggcggg cgaggcggct ctcggtcatc
     2161 tccgatcgcg aggccttccg gcggccgctc ggcggttatt tccgcgatcg ctacgagaag
     2221 ccggacccgg atcgcggccg gatccgcgtg ttgttcgtct cgccttaccc gatccacccg
     2281 cccgttcatg gcggcgctgt ttttatgagc cagactctgg ctgagctttc ggcgctggcg
     2341 gaagtacacc tcatctgcct tttagacgaa aagcgtgatc tggtgacgaa ccggctgctc
     2401 gacggctgct gtgccagcac cgagtttctg gtgcgcatgc ctccggagcg caatggctgg
     2461 agtcctctgc tgccgcacgc ggcccgtgtc ttcttgagcc gggatcttga atggcgcatt
     2521 caccggacga ttttcacgca gaaaatcgac gtcgtacagc tcgaatatac gcagcttgca
     2581 gtgtatgcga tggaattcaa gcgcctggcg acctttttgt ttgaacacga tgtctatttc
     2641 caatctctgg cgcgggcgct gcgaaccgaa cggagcccgc tccggcttct aaaaacaagt
     2701 tttgaatatc tgcgggcgct gcggtttgaa ttgcgcgcga ttgagaaatt tgatgccgtg
     2761 caggtctgca cgcgcgaaaa caagaccttt ctggaacctt ttctgagcag taagcgcccg
     2821 ctgctcgatg gcctccgggc gggaatcgac gtacgccgct atcgctttgt ggcggaaggc
     2881 cggcagccgg acaccttgct gttcgtggga aacttccaac acctgccgaa ccagcaagct
     2941 ttggccttct tctgggcgcg catttggcct ttgatccagc gggagaggcc gcaaacgcgg
     3001 ctgatcattg tgggggcgca ggcggggccg ggttttgccg agttttatag cgccggggga
     3061 gccgagttcc tcggatatgt ggaggatatc cggcagccgc tggaacagta tgcggttttt
     3121 gtatgcccgg tgctcagcgg ctcgggcgtg cgggtgaaac tgctcgaagc atttgccacc
     3181 gggattcccg ccgtttcgac gccggtcggc gccgagggcc tggcatacgc cggcggcgag
     3241 atactggaac tggccgcaac tccggaagag tttgccgcca aaactttggc tctgctcgcc
     3301 gacccgggac gcgctcgcgc catggcgcgc cgcgcccgcc gcgaggtgga acagaagtgg
     3361 gacatccacg cactgaccca acggctggaa gcccattacc gcgaggtgct tcgaaagaag
     3421 ctggcggccg ccggcgtgcc gctggcttcc gttgacctgg agcaggcgcg ctcgcgcgcg
     3481 atttaactgc cggaaaccga gaggatgata ttagtctttg ccgctcaagt cctttttcag
     3541 gcgcttgacg atgtcctgcg cggcacggtg gaggccgtgc ggcgagcggt cctcgagctt
     3601 caagtacgtg gaccagatca cctggcgcga ggcgacgtct atgaggaaca tgttccctcg
     3661 ggcgctggcg ggagctcggc gtggaggggg gtcgctttgt ggcgtgttgg cgtccttctt
     3721 cggcttctca ggcgggctta acggagcgta tatcaggttg agcgattcgg caagagccgg
     3781 gtcgacccgc tcgctaaaaa ccgcctcggc ctgcctgggt tcgacgacta cagcaaacgg
     3841 cccctggctg gctagcgttt ccgctaggta ttgatcaaga ccggccgaca tgggggtcac
     3901 ataaaccttg cgcacagagg caagcccggc cggcgcttcg gcagcagagg cgcccaaaca
     3961 aacgccgaga atcagtacaa tcaaggtttt catcgacaac agtatatcac ccgcccggcg
     4021 gctgccccgg cgttgtggat cgcgcatcgc ggcatgtaca aaaaccatcg catcacggtg
     4081 atcattccat gcctgaacga ggagcagggc atcgagcaga tcctccgacg catgcccgaa
     4141 tatgtggacg aggtcattgt ggtcgataac ggctcgactg atcgcacagc cgtcgtagcg
     4201 caggggctgg gggccacggt aatccgcgag gacgtgcgag gttacgggcg gtcgtacaag
     4261 aaaggatttg cccacgccac cggggacctg atcgtcaccc tcgacggcga ccattcgtat
     4321 ccggtggacg gcatttcgta tcttctcgag gcgttcttgc accttcaggt tgactttctg
     4381 aacgcctccc gcttccccgt tcgcgacccg cgggcgatga gcttcaaaca taagctgggg
     4441 aactgggcgt tgtccctggc cacgtcgctg ctatatttcc ggtgggtgcg ggattcgcaa
     4501 tccggcatgt gggtcttccg gcgcagcatt ctgccgctca tgaaactcga agcggacggc
     4561 atggctttct cggaggaaat caagatcgag gcgatcaaac acccgaaaat ccgcttcgga
     4621 gagatttcga ttctgtactc ctcgcgtttg ggagagatga aattgaaccc gtggaaagac
     4681 ggtatcgaca acttgctgtt tttgatcaag aagagattcg tttaaaaccg ggctgcgaag
     4741 ggcgaagcct gcggccgccc tccgggcgag cctgccggcc ccgccccttg gcgggggcaa
     4801 agccagcggg gagcggttgc atcggagggc ggcgctgaag gaactcgtga gtccctgcgt
     4861 ttcgtttgtg atcccaaact ggaaccggcg cgacctgctg ctcgctctgc tcgggagcgt
     4921 ccgcgctcaa acgttgcagc ccgtcgaagt tctggtggtg gacaacggct ctcaggacgg
     4981 ttcagtggaa ctcgcccggg aagccggcgc ccgcgttctc gagttgaagg ccaaccgagg
     5041 attcagcttc gccgtaaacc ggggaataga ggccgcccgc ggcgatctga tcgccattct
     5101 gaacaatgat gttgaattgg agccggactg gaccgagaag ctggcggcga gcctggagca
     5161 aagcggcgga tggtttgcga tcggcaagct gctcgactac gcaaaccgag atcggattga
     5221 cggcgcgggc gatgcgattt gccgcggggg cgcggcttgc cgcctcggcc atgcgcgacc
     5281 cgacagtgtc tttttttaca caccgcgtgt gactttcttc ccctccgcca cggctgtgct
     5341 gatgcggcgg ggctttctgg aaagagccgg taatttcgat gaatcgttct tcgcttatct
     5401 ggaagatgtt gacctcggcc tgcgggccgc gctcctcgat atggggggca tatatacgcc
     5461 cggcgctgtc gcataccacc gcggcagcgc caccttggga gcctggagca gcgaaatggt
     5521 gaaatgccta acacgcaatc agattttgct tctggccaag ttttacccgt gggcgctaat
     5581 ctggcggttc cgcatcccga ttttggcggc gcaggcctta tgggcggcca tggctgcccg
     5641 gcgcggcagg cttctggctt ggttacgggg cgcggcatcc gggttgggat ccagcctggc
     5701 agtgcggcgt tcagccgttc gctggcgcaa agatggcaac cggcttgctg agattctgct
     5761 gcgatccgaa ggcgaactgg tccgcgtgca gagcgctgcg gggtgggatg attactggag
     5821 gtggtatttc cgcctggccc cccatccgca ggagcccgct ccgtgaatct ccccgtagcg
     5881 gccatagtag tcacatacaa ttccgaattg gagattgaca attgtctcaa gtcgttggag
     5941 aatgtcgcgg aaattgttgt ggtggacaac ggctcggcgg accgcacgtg tgaagttgcg
     6001 gccggacgcc ggatcagagt ccagttgatc gctaattcgg agaatcgggg atttgcgggt
     6061 gcggccaatc aaggcgcgga ggctaccagc agcccgctgt tgttgttctt gaatcctgac
     6121 gctgccgtgg tgagcggcct ggaagcccta gtgcgccaat ttggagaccc gagcgtaggc
     6181 gcagcaaccg gacggctggt tgacgcggcg gggcgcgccc agctcgggtt taatgtccgg
     6241 tcctttccga cgccggccag tctggcgtgc gaactgctgg ctgtaaatcg gctttggccg
     6301 ggcaacccgg tcaaccggcg gtaccggtgt ctggatatgg attatcaccg cccgcaaaat
     6361 gtggagcagc cggccggcgc gtttttgatg gtgcggcgcg aggtcctccg gcgcgtgggc
     6421 ggctgggatg aaaggttctt cccgctttgg tttgaggatg tggatttgtg ccggcgcatc
     6481 gccggggccg gctatagcat ctggtacgta ccgggctgcg tggcgcagca tcggggggcg
     6541 cattcactcg cctcggttcc ggccggacaa aggcgcctgt attggtatgg tagcctgtta
     6601 tcttacagca acaagcattt ttcaccgtcg gctcgagccg ccatccggct ctccgtattg
     6661 ctgggagcat ccctacggat ggcggcctcg cttgtgcgcg ggcgcgagtg gcagtcctat
     6721 gaaacagtaa tgagaatggc gctgaggcgc cgaaagcagg agacgcgaca ggttcaacct
     6781 catgtactca cgtgacctag tttctgtaac catcgtcacc tacaatagcg gccgtttcat
     6841 ccgaaggtgc ctcgaatcgc tgctcgacca gaaatatccg aagttggagg tcattatcat
     6901 cgataatgcc tcgaccgacg gcaccgccga cattctggaa agctttgaag atcgctgcct
     6961 ggttatctac aatgacgaaa atctcgggtt tgcagcggcg caaaatcagg cgattgggct
     7021 gtcgaatggc aactgggtcc tcactttaaa tcctgatgtt tttttgcttc ctggcttcat
     7081 tgaggcgtta atcgaagggg gccgcctcga tccgcgggcc ggaaccgtat gtggcaagtt
     7141 gttcaccatt tccgcaggat ttgatattcc cgataagccg gtcgtggact cgacgggcat
     7201 ttactttacg cccatgctgc ggcacttcga tcgcggaagc caggagtgcg acaacggaca
     7261 ctatctgcgc tatgagtacg ttttcggagc gacggcggcg gcggcctgct accggcgcga
     7321 gatgattgaa gatgtttcca tcaacgggga attcttcgat cctgattttt tctcctatcg
     7381 cgaagacgcc gatctggcat ggcgcgctca attgctgggg tggaagtgca tttacgttcc
     7441 gtatgcgcgc gcgtatcacg tcagaaagct gagaccgggc atgaggcgtt cggtatcgcc
     7501 ggaaatcaat atgcactcgg tcaaaaaccg ttttctgatg cgcctgaaga acatcactcc
     7561 ccgcctgtat ttgcgccatc tgttgccgat cacgatgcgc gatctgggca ttattgcgta
     7621 ttgtctcttg agcgagcaga gctcgatcaa ggcatttttt attacggcgc aaaaactgcc
     7681 gaaagttctc gaaaaacgcc gctggattca agcccggcgg cgcgtagatg acgcttacat
     7741 tgagcagtgg ttccggtctg ttcctgtcag tcttccggta gagggttacg cggagcccgc
     7801 cgaaccgaag gtaagcgctc tccacgggta aggtcaggcc cgccgaatcc agacgcaagc
     7861 aaaaccgctt tagctgaggc gttccgcccg gaaaggcggt ccccgccgct ctctttatcg
     7921 agctgctgcc ttatcatgaa gcctctatga gaattgcgct gctcggaacc cgcggcattc
     7981 ccggccgtta cggcggcttc gagacattcg ccgaagagct ttcaatccgt ctggtcaggc
     8041 gcaatcatca ggtcactgta tactgccggc ggaatcggat gtcccccggc caaagcgaat
     8101 accgcggcgt ccggctggtg acgcttccga ccatcgcgca caagtatctt gacactgccg
     8161 cacacacctt tttttccacc ttgcacctgt tagttcgccg ggccgatgtg gcgctatact
     8221 gcaacggagc caacaccatc ttcaccattc tgccgcgctt ggcgggcgtc ccggccctca
     8281 tcaatgtgga cggcctcgaa aggaacagaa agaaatggaa tgcgctggcg cgggcctggt
     8341 acgccttttc cgagcgcttg gcgacgctgc tgccgacggc ggtcattacg gacgcccagt
     8401 cgatcgccga ctattatctg gcgcgttata accagccgac atactttatt ccttatggcg
     8461 ccgcaacggg ccaggtcgag ggaaaggaaa ccttggaccg cctcggaatc gaaccgggca
     8521 agtactggct ttacgttagc cggatggagc ccgagaacaa tgctcatctc gtcgtgcggg
     8581 ccttcgagaa aacatcggcc agcatgaagt tagtcatggt cggggatgcg ccctacgccg
     8641 gcgagtacat ccggcaggtc cgcgccacac gcgatccgag aattctgttt ccgggcgcca
     8701 tctatggcaa cggctatcac caattgcagg cccactgctt cgcttacatt cacgccacgg
     8761 aggtaggcgg tacgcacccc gccctggtgg aagccatggg gcgcggccgg ccagtgctgt
     8821 atctcaataa tagggaaaat gcggaggtcg cgggcggcgt gggcgttccg tttgacgccc
     8881 tggagtcttc gctggcggac gcgatcgagc gcgtggcagc catgcccggc cctgagcgcc
     8941 agaagctcgg cgaactcgcc ttgcagcgcg tacgcgagcg gtacgattgg gagtcgatca
     9001 ccgaccgcta cgagcaactg ctgcgaaagt atgcggtctc gtcaggctga tgcggctgcg
     9061 cccggcggga tatactgatc agtctctcgc gagcgcgatc tggcggcggg caaaatagaa
     9121 atttgcaggc tagcgttagg gccaggccgc acgagagaaa cgaacatgcg cagtgggatg
     9181 cgaaggccct gtttttgctt actgttttcc tttgtcgtct tgccgctggc ggcgcagagc
     9241 agcagaccgc ccgtcgtgct ggtgaacgga tacgacgtgg gctgggcttt gcggggcgcc
     9301 tgcacgccgg cagccgggtc gcgagacacg ttcggatccc tcgaagattt cctcaaggca
     9361 gacggagcgc cgcaggtgta tttttttgat aactgcgccc agtctggcca acagactctg
     9421 cccaacccga gtattgaaga actgggccgc cgcttcggag attttctggc gaacacagtc
     9481 gccgcgccgc aggtggatgt ggtagcgcac agcatgggcg ggttgattgt gcggtcctat
     9541 ctttcgggaa agcagcctgc cttcggctct ttcatgcctc cggccggcac gaaaatccgc
     9601 aaaattgtat tcatcgcgac gccgcatttc ggcgccccgc tggccgggat cgtcggcagc
     9661 tcgccgagcg acatgcagat tcgggaaatg gcgccgggca gcgccttcct ctgggatctg
     9721 gcgacctgga atcagggctt cgacgatctg cgcggcctcg acacgctggc cgtgatcggc
     9781 aatggagaaa gcgacacaaa gggcgacggc gtcgtctcgc taaccagcgc ttcgctcaac
     9841 tccttactgc aaccggatca gcggacgcgc atcgttccct attgccacat ctcaggtctg
     9901 tttttcttcg gctgcccagg accggcgatc gccaatgccg attcgagcga ccagcttaca
     9961 gcccagatcg tgcgctcttt cctggcaggt ggaaccgcct ggacgcagat cggcgaaact
    10021 cccagccgaa acgccgtttt gagccagcag ggcggggttc tcttcggaat gaaggacgca
    10081 tccgactcgt tccaaactcc cacacaggtc tcctacaaac agtcgcggga tgcgtctatc
    10141 tccggccaac tgtcggcggg gccgtctttt ttctacgccg acctgcttgc tggtggatcc
    10201 tatgtttttg acgcgcaagc tggcgcccaa tcgagtgatt ttttggggac tctgccgccc
    10261 ggcggcttcc gggtattctt gggcaaggcc ggcccggtga tgaagctcat cgttccggcg
    10321 gcgggcgtgc cggacgtcct ggctctggcg gccggttcga tcatatcgat ttacggctct
    10381 aatctcgccc aagggaccaa gcaagcttcg acacagccgc tgcctgccga aatgctcggc
    10441 acgagcgtcc tggcccaggg ccggcctctc ccgctgctgt acgtgagcgg gcagcagatc
    10501 aacgccgttc ttcccgccgg gttgagcggc ctaacgcccc tgaagctgct cacccccggc
    10561 ggacagcata ctttgaacgt ctggatttcc ccggccgcgc cggccgtttt tacactcgac
    10621 agttcgggct cgggccccgg agcggttctg cacgcctcta ccaacttgcc cgtgacagcg
    10681 tccaatccag ccgagccggg agaatacgtg tcgatctacg ccacgggcct ggggccgacc
    10741 atgcgatccg gagatctgga cgtggcgatc ctcacaccgc aggttctgat cggcggcgct
    10801 ccggcaacga ttctatttgc cgggaacgct cccggctacc tggggttaaa tcaaatcaac
    10861 gtgcaggtgc cccagattgc ggctggaccg gctgttcccc tgattgtgca gagcggcggc
    10921 cacagcagca acgtggtgac gttggcgatc cgataacgga cgaaaggcgg ttgcttgttc
    10981 cgggcgccgg gctgccacgg attccttcgc ccaaaagctc gcccggaacg atttgggatc
    11041 ttctttggcg accggatcag gctttcagga cggtcagcac ttcgttgagg gatccctgcc
    11101 ggtacccttc cagatccaag gtgacgtact ggaagcccag attcttgaag atgaacgtga
    11161 gacggcgcgc catttcaagg ttgagggccc gatcgagctc atcgggagcg acctcgatac
    11221 ggactaactc cccgtggaac cgcacccgga actggcggaa gccgagagcc ttgatcgcct
    11281 cctcgccttt ttcgacgatt ttcacattct ctttagtcac aggagtgcca tatggaatcc
    11341 gagagctcaa acacgccgag gcggggcggt cccaggtggg cagccccatt tgccgtgaaa
    11401 gctctcgaat ttccgccttc gtgaggcccg cctccagcag cggagcgctg acgcggtgct
    11461 cctttgcggc gcgctggcct ggccgaaagt cgcccgtatc gtcaacgttc acgccgtaag
    11521 cgacgtaggg gatgccgcgt tcccggcgaa tcgcctccag gcgcgtgaac agctcatctt
    11581 tgcagtgaaa acagcggtcc gggctgttcc gcacgtaatc gggattctcg aattcgaaag
    11641 tctgaatgta ttcgtggcga aggccgaacc ggcggacaaa ttcttccgca tcccgcttat
    11701 gtgattccgg aatggaagcg gaatcggctg tgaccgccag ggcgccgtcg cccaaaacat
    11761 ggtgggccgc ccaagccagg tatgccgagt ctgtgccgcc ggagaaagct acaatcacgc
    11821 gccccatgcc gcgcagaatg gaaaacagtc tctcctgctt ttgcgcaagc tccggctgca
    11881 tacaggctca ttatagcgca agcgccgcag cgttccggaa ttgctgcaac cgcctcctca
    11941 tgagcgcaag cgccgttcga cggccgaatc aaccgcgaac attcgttgcg attctaacag
    12001 gtcaatcgaa tcgcaggagc ttgggttctc ctccgcgctt ttcgaaattg attcgcgaac
    12061 ctgcctctgc tacgctgatc ggtccatgga cgagccgccg cacatcagcc tccgggatga
    12121 agaagaaccg cctcccgtcc tcggcacctg gcgccggctg tacgcggcga tcgcgctgta
    12181 tctgcttctg ctaatcgtgt tgttctatct tttcacggtg acgttcaacg gagctcgatg
    12241 aggaccctgg attgggcggt gctggtagga tcgctggttt ccatcgtcct ctacggtctg
    12301 tataagggcc ggggcagcag cacggtggcc agttatcttc tggcgaacaa gacgatgccc
    12361 tggtgggcca tgggcttgtc catcatggcc acccagacca gcgcgattac gttcatcggc
    12421 acaacgggcc aggcgtacgt ggacggcatg cgctttgtgc agttttattt cgggctgccg
    12481 ctggccatga ttgtggtctc ggcggtttcg gttccgttct tccatcgcgc cggggtttat
    12541 accgcttacg agtttctgga aaagcgcttc gatagcaaaa cacgcaccct caccagcatc
    12601 gtatttctga tacagcgcgg gctgagcgtg ggcgtttcgc tctccgctcc ggccgtggtc
    12661 atgtcggtgc tgctagggtg gtccgatcgc aagaccattc tcatcatggg tggcctggtc
    12721 atcatctata ccgtgttcgg cggcatcaaa gccgtcacct ggaccgacgt gcaacagatg
    12781 acgatcatgt tcctcggtct ggcgacggcg ttgacgatgg cgatcgtctc cttgccgaaa
    12841 ggggtttcct tccgcgacgc tctttattta gccggcgcct cgggcaagct gaacgcggtg
    12901 gatctttcct tcgattggaa cacccgctac aacctgtgga gcgggctgat cggcggcatg
    12961 ttcctggccc tttcctattt cggctgcgat cagtcgcagg tgcagcggta cctcaccggc
    13021 aaatcgatcc ggcacagccg catcagcctc atgttcaacg cggtggtaaa ggtgcctatg
    13081 cagtttttta tcttgctcac cggggcgctg gttttcgtat tctttctatt cgaacagcct
    13141 cctatgctgt tcaatcgtgc ggagcgggag agagtcgaat cgaccgacga gtatcgcgcc
    13201 gccgagcagc gatacgagca gacctttgct gagaggaggc gcgcggcggc ggcgctgatc
    13261 gcgtcgcggg acgccgccgc ccgacggcag tatctttcct cgctgaagga tttggagacg
    13321 gcccgcgccg cgggcatcga tgtcgtccgg cgcgtcacgg gaaccgagta caacgataca
    13381 aactatatct tccttacttt tgtgaccagg tatctgccgg aaggcctggt gggggtcgtc
    13441 atggcggctg tatttgccgc ggccatggga agcattgccg gcgaactgaa ttcgctggct
    13501 acggcctcag tgatggacat ttaccggcgc cacataaagc gccagggggg cgacgcccat
    13561 tatctggttg tgtctcgcgt agctacgggc ttctggggtc tttatgccat gatcttcgcc
    13621 atctttgtca cccggctggg atcgctcatt gaggctgtca atttggtcgg ttctttgttt
    13681 tatggatcgg tgttgggagt gtttgtgctg gccttcggcg taaagcgcgc aaacggcaac
    13741 ggcgccgtct cgggccttct ggccggcctg gcgactgtag cctggctgag ccgcaatacc
    13801 gggatttctt ttctctggta caacgtggcg gcctgcgtgg ttgtagtggt ggtgggcatt
    13861 cttgtcagcg ggctccgacc ggcgtccggc ggccccggca cagtcgaggt gaaatgaccc
    13921 ggctgcggtg cgcgttgctg gcgctattgc tgctctcggc cagttccggg tgccgcacgc
    13981 agtcccggcg cgtggtgggg ctggcgccga aaggcgtcaa tcatatcttc tggcaaaccg
    14041 tgcatgccgg cggcatcaaa gccgcgcagg aattcggctt cgagatagag tggaacgctc
    14101 ccgcgctcga gatcgattcc agccggcaga tcgaaattgt ggaatccatg atcaatcggc
    14161 gtctggccgg catcgccctc gcgccggtgg ataagcaggc tctggtaggg gtagtagagc
    14221 gcgcctggcg ggaaggcatt cccgtgtcga ttttcgactc cgatatcgac accgataagc
    14281 gcatcgctta tgtggccacg gacaacttgg agggtggaag aatggcagcc cggcggctgg
    14341 gcgaagtcct cgagggcaag ggaaaggtcg ggattgtggg cttcatggcc ggcagtgctt
    14401 ctaccatgga acgcgaaagg ggatttcagg aggaaatgcg cgcgcgattt ccggacattg
    14461 agattgtagg gctgcaattc ggcatggcgg accgggctaa ggccatggcg gtcaccgaaa
    14521 atatgctgac cgcacacccg gacttggccg gcctgttcgc cgacaacgaa tcgagctcga
    14581 tcggggctgt gcaggcgttg aaatcgcgcg gcaacaaaac cgtcaaactg accgcctttg
    14641 acgcttccga ccagctcatc aacgatcttc gcgacggttg gatcgattct ctggtggtac
    14701 aaaatccgtt caggatgggc tacgaagcgg tgcgggccgt gggcctgaag ctcagcggcg
    14761 ccgagccgcc ggccagagtc gattccggag caaccctggt cctgcgaaaa gatttggaaa
    14821 ggccggaagt gaaggcgttg cttttccccg atatccgggc gtatctgcga gcccgttaac
    14881 ggcaaggccg ccgctccagt tgatttcctt cgcgtccacg gcgccctccg cgagttacaa
    14941 tctgtgcgaa cgccgggcga ccccaaggca ggctcggatt gccccgttcc tcagatctcg
    15001 aagccgcatg aagtgatacc atagactgcg tgggccggct tccggcgcaa gcttcggaat
    15061 agacaacttt tatcaactga ttcacaattt tccacttgat cttttcccgt tctctgcgtc
    15121 atcctatacg gttttcgcac tcgggggcgg ttttccctag tcggccttga tgcgcgttcg
    15181 tcttttctat cacgaccggt gctttgacgg agcggcttcg gcggcggtgt tcacggaatt
    15241 cttccgcaat acctacgacc gctcggcgga attcgtgtgc acaggcttgg cccacaaagc
    15301 gtcccagcta tttgatgaag cccagttcga cggcgacgaa aatgcgatcg tcgatttcaa
    15361 gtactccagc tccccgcgcg taacctggtg gttcgatcat catcaaagcg cgtttttgag
    15421 cccggaggac gccgagcact tccggcacga caaaagcggg aaaaagtttt acgatccgac
    15481 gttcaaatcc tgcacgaagt ttcttgccac catcgcccgg gagaagttcg gcttcgtgca
    15541 gccccggctg gacgagttga tcgagtgggc ggacatcatc gacggcgcgc agtacccgga
    15601 cgccaagacg gccgttgaac tcggctcagc cgcgatgaag ctgactctgg tcatcgaggg
    15661 cgccggggac agcatcagcc aaaagatgat ccgctggatg gcctcccgca gtctggatga
    15721 catcgccaat gagcccgagg tccgcgcctt gttcaatccg ctctatcaga ggcatctgga
    15781 gtccattgac ttgatccgcc agcgcgccga ttaccagcgg ggcgtcatct tcttcgactt
    15841 agtggattgc ggccatgaag gctacaacaa gttcatcccc tactacttgt tcccggacag
    15901 cctgtacacg gtttccgtaa acacctcgag ctttcgcacc aaggtgtctg tggggtcaaa
    15961 cccttgggcg ccgcgcgagc cagggcacaa tctggctacg atctgcgagc gctacggcgg
    16021 cggcggccac gcgcgcgtgg gagcgatcag cttcgaaccc ggcgcactgg agcgcgccag
    16081 aaaggccgcg gctgaaatcg tggaagagct tcgcagcgag agttgacctc catgcgggct
    16141 cggtcggcca catccgatga cgccccggcc atcgcccgaa tttacaacga aggaatcgaa
    16201 gaccgcaccg ccacgtttga aacacgcgca cgttcggcgg gcgatgtgcg cacctggttc
    16261 gacggcttgc acccaatcat catcgttgag gagcgcggcc ggcccatcgc ctttgccgct
    16321 actttttctt accggaaccg cgagtgctac gcgggcatcg cggaaacatc cctctatgtg
    16381 gcccgccagt ggcgggggcg cggggcgggg cgtctggcaa tggaagctct gatcgaagcc
    16441 gccgaggaag ccggcttttg gaagctgctc tcccgtgttt tcccggagaa cgaggcgagc
    16501 cgcgcgttga tccgctcgct cggatttcgc gaggttggga tttaccacaa gcacggcaag
    16561 ctcgacggga tttggcggga cgtagtcatc gtcgagcgcc tgatcccgaa aaatctgaaa
    16621 tgatggcctg ggcgaggccc ccaattctgt tgatgcttgc cgggcggaat ccggcccgta
    16681 gagcttttgg ggtccaccgg gcgcctcccg cgcgggcgta gcgcgttctt caatcgccgt
    16741 gagggacttg taaaaggctc tcggcttttg aaccggatcg gacaccgtcg ccattttgag
    16801 ggaaagcggc cacgcagcct gcctgggttc aatgcgggat cggtgtgact tccctgtagc
    16861 agagaatggc atcgtaggaa tccgcccagt agacaaggct ggcttccagt aaggaacgct
    16921 ggatctccgg aatccgatga agagcctgcc gaatcggccg cagatcgaaa acagtggccg
    16981 gatattgcgc tagcgatgcc aggaactcta atgctggatc atccttgcgc tcatcggcct
    17041 cgagcaactg cccactccag taaatccggc cgcccgcggc aaacgcggcg acattgaatg
    17101 cctgcaatcc tcgagcaacg gcgagttctg ctacaaagtt tcccagcgtg gaaactcccc
    17161 gccggtcgta gcccctatga aggtgattcc gcccaaaacg caacagcacc ttcggctggg
    17221 ggctgctctg ccagtgccgg tagaaaagct ccttcatgag cgcttcccgg cgaatcgagg
    17281 cctcgagccg ggtatcggcg ccgagacgag cgatctcaat ctccagagtc cttaagatat
    17341 tactgcgcag cgaaacgccc ccggcgaaaa gatcctttac tccggtcgcc tctcgcaacc
    17401 gttgaagcag ctcgcgagcc atgctccggc ggtatccgag gcgggtcatt tccaaagccg
    17461 actggaggtt ccggtttccg gggttcgcag cggccaggtc gcgaatggga aggttcggtt
    17521 gcgcctcctc gatatcgcag ccccaaagaa cggctccccg gccgcggttg aacgaggcgg
    17581 cgaaagttgc ctctgcctgc gaccataggc ccctgatcgg ctcgctggcg ccgggaggtc
    17641 tgaattccaa ccggttcgcc gcccaagggc tgagctcggc agcgatatgg cggtagccgg
    17701 cttgccacat gggaggccag atcatccgca gcagagcggg aatctccttc tcgccgtgga
    17761 gttcccccag catgaaaaaa gaggcgcttc cagcttcctt caggagaaac acccggcctt
    17821 gcgtcgagag gtcgtattca tgctggcgaa tcgtttcaga aacggttccc tccgtctcgc
    17881 agacggcgag tctttcaccg gaaacgaagc aactcagagc cagcagccac aggccgaatc
    17941 ggcacacacg agctagtacg aaggccgcct cgcaaaagtt ccgcggcgcg cggacaaaac
    18001 ggcccgggct gggaccggcg caggaattca atccattggg aatttgatgg ccggccgaga
    18061 gtcaggatga ttctttcgcc aggcgcccgc cgcgctggag gcggaagctc tggctccgcc
    18121 acacaacccg ccgcccgaaa aggctggccg cccaaaccgc gaaactggcc aaatcggcga
    18181 gcgggatcag gagaaaatag cgaaccacgg ccctgtcccg cagtttcata cagccaacgg
    18241 caatggccgc cgccgcgcgc agccccaggc ataacatcgc ctcgggccac aaggacggct
    18301 ggagggccac agcggcaatc acgatcggta cggcgaacgt gatcgccatg ccggcataac
    18361 cggcgggccg gcagacccgc agcgtgcgcc cccagcggag gcgatgacgc caattttcag
    18421 cccagggctg gcgcggcagc attgtctcca ccacacaatc cgagagagca atatcgaagc
    18481 ctcgggcggt gattgccttt ccgagctgat aatcgtcggc caaataatcc gccaggggag
    18541 caaagccgcc gatttgctcc aaatcagcgc ggcggagggc catggtagcg cccaacgcga
    18601 agcgcatacc cagcgcccgc gcaaccagaa cgcccggctg aaaatccgtt gagatccaga
    18661 gcccctcgag tacggaagcc aaccccccgg cgggcacgcc gcgatagagg caagtaacca
    18721 ggccggtctc tttcttctcg agcggagcga cgacgcggcg cagataatcc ggacctgcta
    18781 ggatatcgct gtcattgatc accaggatgt cgtagcggca ctcggcgcgc agccgctcga
    18841 ggctgttcac cttggcgttg gcgccataat cacggctggt gaaaaacaca tcaatcctgc
    18901 gctgcggaaa ttccgcggcc aggcggcgaa tcgcccccac ggccggatcg gccggatcgc
    18961 gcacggcgaa cagcaactcg aactcgggat agtcctgcgc ggcgtggctg cggatcgccg
    19021 cgtagaaatc gggatccagg ccgcgcaccg gtttgagcac ggagaccgga ggcgtgaatg
    19081 gccgagcatt tcttccgggc cgcggccgcg cgcgcccgag cgcggccaga agcgccgcca
    19141 ggtgatacaa ggagcaaaca gccagcagcg ccagcaagac gagctcgggc agcctcacaa
    19201 agagatcaat accacgccga tggctaccag tatggccccg agccaccgct ggcggctcac
    19261 cgtctccttc aagatccaaa cggcaagcac cgtctcgatc acgtaagccg aggcggttgc
    19321 gggcacggcg aagctcaggt cggccaccga aaccagcccc ataaagccga agaaggaaac
    19381 cgcgaacgct gcaatggaaa gaatgatcca gcggttgcgt atgatgacgg ccagagagcg
    19441 gcccagcggg cccggccgga agtcgtcgat gatctcgtgc tgcttcatgc cgacagtcat
    19501 aaacacgtcg ccgagggagg ttcccagcac gaccagggcg agcaagaaaa cgatcatcat
    19561 aaacgggcct gcgattggtt ccgctccggc tccgcgcttc gtttgggcaa agtgctatgc
    19621 ggagttccac ccaccaaaac cacgcccgcc gtaatcacca ggatgccaat ccaccggagc
    19681 ggcttgatcg gctcgtccaa aagccaccat ccgagcgccg cgacgacgat gtacccggct
    19741 gaagtcatta ttaatacgta actgaggtcg gcccagctca aaacaatcat gtgggctatg
    19801 aagaacacga ccagcagcgc cacgcccagc agaacaaaag ggttggaaag agcgcgcatc
    19861 gaagaaacaa tcaatttcaa cggcgaataa gacgcgatgc tcccgatttg ccgcatgcca
    19921 tagccaagca ctacgttgcc cagggcgttg ctgaggacaa taatcaggag gcaaatacga
    19981 gtcttcaaga ccgggaggtt cttttaatcg ccggcaggcg cggccggaat cggacgcggc
    20041 tgcggcggct gcacctggcg ttgatcgagc acaaacttct tccgcttcgc gcgcagggcc
    20101 aggtattcac gtgcttcttt ggccagccgc cggcgctccg ccccattgaa aagggccttc
    20161 cgtacgatac gccacacgac tttcggccgg aaataaaact cgccgtagaa acgctccacg
    20221 gcatctacca gttcaccacg atccaggccc ggatagatga cgtttggcaa ctggtggccg
    20281 gtgtcgtcgg tcatcgaatc gattgtgatc aggtcgttct tcttgacgta ttcgtaaaac
    20341 tccgtgccgg ggtaggcgtg cgcaatggaa acctggatcg tctccgtatc caggcgcttt
    20401 gcaaactcga tcgtagcgtt gatggattcg cgagtctcgc cgggcaggcc gatgatgaaa
    20461 tccccatgga tcaccagccc cagctttttg caatcgcgcg tgaactcgat cgcccgctcg
    20521 acggttgcgc ctttcttgat gtttttcagg atctgcggat cgccggactc gtagccgaca
    20581 atcagcaggc ggcacccggc gtctttcatc gccttgagcg tttcgtaatc caccgtaacc
    20641 cgcgaagtgc aggaccaggt gaacttgagg ggcttcaact tctcacagag ctcgagcacc
    20701 cgggatttgc ggatattaaa ggtatcatcg tcgaaaaaaa tctcctttaa gccgggaaag
    20761 ttctccaggg cgaaccgcac ttcgttggcc acatcattag cggagcgcgt gcgccacggg
    20821 tggccggaca gcgtttgggg ccacaggcag aaagtgcaca aagccgggca gccgcgctcg
    20881 gtgaaaaacg aaagatatgg gttgagcaga aagggaacgt tatagcgcgt gatatcgaaa
    20941 tcgcgcttgt aaaccttggt cacccagggc aacgaatcga gatcctcgat cttcggaccc
    21001 tccgggttgt ggcgaaaccc gccgttttgg cgaaagctca cgccgggaat ctcttcgaga
    21061 ggcttcccgt tcgcgaaatc gacaatcggg tgatcgaatt cacccctcac cacaaaatga
    21121 atggccttgg aggctttcag actttcttcc ggcagaaccg aaacgtgcgg gcccacaaag
    21181 gcgatcttaa gccgcggatt cgcatcctgc atcatctcgg ccatgcgcac atcgctgtga
    21241 aaaccgggcg tcgaggtgaa cagaacgaga aactcgaagt ccttggccat ctcgatggtc
    21301 tgccggacgg aaatcttgtg cggcggcgca tccaccacct tgctgtcggg cagcatgccg
    21361 gcgggatagc acagccagac gggataccag taggactcga tctcccgggt ggccggccag
    21421 cgcgagctcg cgccgccatc gaagccttca aaggaaggcg gattcagaaa gagtgttctc
    21481 atgatcttgg cttgcgccta aaaaagagac cttatcagac ccgctactgg ctgtcagccg
    21541 gaatcagccc gacttccatg gcggacggct caaatcggta gtccgcgtat tgaactgtca
    21601 tatgcgcggg gccgacgact ctcgtctcca ccgtggtctc gagacgcgcc accacagcta
    21661 cgccatccgt ggtctggtaa tcgcgggtaa agtacacctt ttttataaaa acggaagggt
    21721 tcttaacaag aaaaccagat tcccgcacag ggagaaaagt ctgggcgtcg atccacagtg
    21781 ttcccttaaa caaccctttg cgcttgtggc gaggcttcag agcgaaaacg tgtacacttc
    21841 gcccctccac ggcataaacg cctttatagg aaaacttata gttttccggg ttgatcgcga
    21901 gcgatggatc atgcgagtcc cggacttgca cctcggtcga caggtaccgc gcaatcacgt
    21961 catttttgac cgtgttatcg ccctcgaagc gcagaatatc gtaggtcagg cgtcccagcc
    22021 gcgagacccg gcgcaaagcg gagagcctgc ctcttttgtg caacttggga atctcggcat
    22081 cgatgctgac ctcggccgta gagccgttca accggtcttg ctggctgcgt gtgacggcaa
    22141 ggtacgagtt caggatctgg acggaatcgt aatggggagg ttccgccgcc aagcttccaa
    22201 cgaacagcaa agcgagagct agaaatgatt tactggcgcc actacgcatc actagaccct
    22261 atcggagcgc aaattgtgta ttgtacgaca cgtaccggaa tacccgcaag ctcaagacac
    22321 gcgaccgtcc ggcgcgtccg gctaaactga caaccctcgc ccaaacgctc gaaacagata
    22381 ggatgcgcag cgctgcccga agttacataa gatccggaca cccaaaagaa agaagcgaag
    22441 atttaacgaa aagaccccta gtcgatcagg ccgctgcgca tggcgaagcg caccagttcc
    22501 cccatgctgt gaaggttcag cttctccatg atccggccac ggtgagcatc cacagtatag
    22561 acgctcaggt tgagagcggc tgctatctcc ttgtttgttt tgccctccgc aatcatctgg
    22621 agcacctcgc gctcgcggct gctgagaagg tccagaggat cggtcacgtg ccgccgataa
    22681 tcgctgagga cggcgtcggc gacgcccggg ctgaggtatc cttctccacc ggccaccgag
    22741 cggacggccg ccagcagatc gcgatcgacg gaatccttca acaagtaccc gcgagccccg
    22801 gcgcgcagaa tttcccgaac gtaaaccgag tctttgtgca tgctgagcgc gaggacgcgc
    22861 gttcggggag ccacgcgagc cagccggcgg gtggcttcga tgccattcag ttcgggcatg
    22921 gccacgtcca tcaccacgac atccggaagc agcccttgcg ccaactccac ggcttcccgg
    22981 ccgttccccg cctcgccgac gatttccata tcgggttgag ccgtcagaat gagccggaat
    23041 ccctggcgaa ccaccggatg atcgtcagcc agcagaatac ggatcttctt cgtcatgact
    23101 gaagagccgg ctcccgtcct accgaagcgc catcatcggc cagcttggcc ggcacttcta
    23161 ctttgatgac cacgccttgc ccgccgccag accggatgga gaactctcca ccggcatcgc
    23221 gcgcccgcgc ccgcatgccg atcatgccca ggcccccatt acgcggcgat tcgctggaaa
    23281 ggcctcgccc gttatccgca atcgaaagct ggatagcgcc atcccgattc tggagatcca
    23341 aacggacccg ggtggctccg gaatggcggg cgacattggt aagggcctcc tgcgcaatac
    23401 ggaacagatg ggtctcggtt tcgtcggcaa gccggcccgc gaaattggat tggtattcca
    23461 ccgcgatgcc ggtccgctcg gtaaacctct cgccaagcca gcgcagcccg gcatcgaggc
    23521 cgaaatcgtc gagaatgatg ggccgaagca actgggagag ttcgcgcaca ttctggatcg
    23581 cttcgtccac cagatgggcg cagtcggcca gccgctcagg agcggggctc ggatccacgg
    23641 cgagcgccgc caggttcgct ttcaccgcgg ccaaagactg ccccagctcg tcgtggagct
    23701 cgtgagagaa gcggcgtgcg gtggtttcct gattttcgag catgtgccag gagacccggc
    23761 tgagctctcc ggtctgccat tccatttttt ggaacaaggc ggcggtcatc cgcatggtga
    23821 gcgtggcgca gaccagcgcc agcaccaggc atgttccaag cagcagcagg gacttggtct
    23881 ccaattcctt cgagcggcgc tggatctgat cttgcgccgc cgaagccttt tgataactcg
    23941 cgttgatcag cttggcgacg gcggcgatga cttcatcgtg ccgccggaac aattcccggg
    24001 aggaaaacga gtgtgcgtcc tcggccgaaa gcagccgccg cgcctccacc gtgaagccgg
    24061 cggacgcgcg ctttagttcc tgccacaggg gatcctcgtc gctgccggaa gcggcggcga
    24121 tcaccttctc gatatgctca tcggattcgt tgagctgcgc cagcacacgc tcccgatcca
    24181 ccgcccccgc cgggcgcgac aacgtgttga aaacagccgt caagttgccc tcttcgcgtt
    24241 ggatttcgtc aatcaggcgc gtggtgacta actgctgcga aaccagattt gcggcgttct
    24301 ccttgatcga gcgcgcgttc tccactccca caaagccggc cgccagtaac aggaggatca
    24361 ccagcgcaaa accaaggacc agcacgcgaa caatgctttt gctggtaata ggctgggcag
    24421 gcattgcaaa atcgccttta cctacagtgt atggcaacac agccagcccc cgcatcaaca
    24481 ctaccacttt ggtccagagc catccgggaa gctatcgcta tcaaaggcta aaatggcctc
    24541 caaaagaagg ggatccggcg gcagggcgaa tcagggacga ggggaaccca tcccccgctt
    24601 tcgggcccgt ctatagcctg ggcgggctat cgttcgagtg attcggcgcg gattaagatt
    24661 taatttacgc gaagtttctc ttgatgtagg ttccgggact atcactgtga atctgagcgc
    24721 gcttctcaac ctgatctggg ccgctatcgc cgccacagcg ctgggttgtc tcgcctatgc
    24781 ggagtttcgg tcccggaatc gcaggcgctc ctgcgtccgc cgcgccgtca gtgtgctaat
    24841 cgcggcgttt ctactgtttc cctgcgtctc tgcgagcgat gactggctat gggtgcgcgc
    24901 gttgccgctg cggccggaga gcggccgggg ggccgggacc tcgcctatca acaagtccga
    24961 cgaaaaagct tccatcaacc tcgcgcgcct gctcgaagcg gtgggcaatt ttcaagttgc
    25021 cggaatctat tcactgccct tcacggcgtg ctttttcttt ctgtttcggc tgcccaaccg
    25081 caaactccga accctgcgtt tgagttcgcg cgccggccgg gacccgccgg ttttctccct
    25141 ttcccgttag tcgcatcgtt tttcggattt ctggagaggc catatgctac gccaataccg
    25201 gcgcgccctt gcctgttttc tgattcccgc gggcctcagc agtatttgct gggcccagaa
    25261 gccgcttacc tgggaggagg tgcgcgagcg ttttcgaatg aacaatccgg ccctgattgc
    25321 cgggcgagct cttattgagg aaagccgcgc gtccgaaatc acggcctacc ttcgccccaa
    25381 cccggagttc acggccaccg tcgatcagat taatcctttt acccccaatc ccaccgccta
    25441 cactccattt gcccaaacct tgccgtttgt ctcgactacg tatctgcacg agcgccagca
    25501 caagcgggaa ctgcggcgcg acagcgctcg tctggcgacg caaatttcga cgacagatca
    25561 ggccgacctc gagcggcaat tgacgtttaa ccttcgcgac gccttcatcc ggacgttgca
    25621 ggccaaatct gttttacagc tcgccaggga gaacctcggt tactacgacc acgtgatcga
    25681 cgtgaaccgg gagcgcttca aagccggcga catctcgcgc gctgatctga tgcgcgtgga
    25741 gctgcaacgc gttcagttcg agagcgacct cgtgaacgcg gaagtgaatc tgcgcacagc
    25801 caagatcgcg ctgctcgcgc tcatgaacga gcggctcccc gtggacgcgt ttgatatcac
    25861 cggcgacttc gacttccacg aaagcagcct gcgactggag gacgcgcgcc gagcggcgct
    25921 tgaagcgcgg cccgacctga agtcggcgac cactgccatc gagaaggccc aggcggacaa
    25981 ccggctggcg tgggccaatg gctcggccga ccccacattc ggagtggacg tggcgcgcaa
    26041 cccgcccatt caggcctacg tcgggttcag cgtcaccgtc ccgctgcgga ttttcgacaa
    26101 gaatcagggc gaaaaggcgc gcaccagcat cgaaatcggc cgctccgagc ggctgcggga
    26161 agccttgtta gccggcattt atcgggacgt cgattcggct tacacctccg tggaaagcgt
    26221 ccggaccctg ctgcggccgt atcgcgacaa ctacctgcct caggcgacgg agattcgcga
    26281 caccgtgtcc ttcgcctatc agaatggcgg cgcgtcgctg ctcgaattcc tggacgccca
    26341 gaaatcctat cgcgacgtgc aactgaacta caggaacctc atcggcagtt atttatccgc
    26401 cgtcaatcag ctaaacctgg ccgtcggccg ggaggtgatc ccatgaagcg gcgtactcaa
    26461 tgtctactgg gcggggcggt gctcggcttg gcgactctga ttttcgccgc ctgctcccgg
    26521 gagtcgtccg ccagcgcttc cattccggat accggaccgg tgaaggtcga agaggcgcct
    26581 gacgccactc tgatccaggt agatcatccg gagcagtttc ctctggtgaa ggtcgaagcc
    26641 cggcgcacgc acgacgagct ggtggtgaac ggcgttgtcg cccccgacgt tagtctgacc
    26701 attcctgtga acgccctggc cggcggccgc gtggtggaga tccgggcccg gttgggcgac
    26761 gacgttaaag cggggcaagt tctgctgcga attcacagtc ccgatttggc cgcggccatc
    26821 gcggattatc aaaaagcgaa ggccgatgaa tccctcgcgc gccggtcgct cgagcgctcg
    26881 cagttgctct attcgcacgg cgccttggcc ctgaaggatc tccagcaagc ccagatcgcc
    26941 gaagacaaag ccaaggtgga cctggatacc gccgccgagc ggattcgtat tctcggcggc
    27001 gatgtcgatc atctttcgcc gatcatcgag ctgaaagccc ctgcttcggg aaccatcatt
    27061 gaacagaaca caagcagcgg cgccggcgtg aaatcgctcg acaactcccc caacctcttc
    27121 accatcgcga atctgtcgcg cgtatgggtg ctgtgcgacg tctacgaaaa caacctggcg
    27181 caagttcgtc tgggtgattt cgccgaagtg cgcctgaacg cttatccgga tcgccctctt
    27241 cgaggcaagg tgagcaatat ctccaaaatg ctcgatccca acaccaggac ggccaaagta
    27301 cgcctggagc tgagtaacgc gagcggcctg ctccgccctg gcatgttcgc aacggcaaag
    27361 ttcatttcac agggcagccg gacgaggccg gtggtggcct ccactgcgat cttgcggctc
    27421 cacgacaagg attgggtctt ccggcccgcg ggagaaaggc gattccgccg ctgcgaggtg
    27481 cagggagggc cggttctccc cgacggctcc caggagattc tagccggttt gcagcccggc
    27541 gatgaagtgg tggcgaacgc gctccaattc tcgagcacag tggagcagta gcaggaatca
    27601 tgctccgtta cttggtcgat ttcgccctgc gggaaaagct gctggtgctg gcggttggcg
    27661 ggatgctgct gatctggggc gtcatttcct tccacaccct tcccattgaa gcttatcccg
    27721 acgtggccaa cacctgggtc caggtgatta cccaatggcc cggacacgcc gccgaagaag
    27781 tcgagcagca ggtcaccatt cccatagaga cccataccaa cggggtgttg catctggctt
    27841 ccatgcggtc ggtttcgctg tttggactct ccgttgtcac cctggttttc gacgacgaag
    27901 ccgataactt gctcagccgg cagcaggtgc tcgagcggct ctcgcaggtc aatctcccgc
    27961 agaacctgac cccgcaactc ggcccggatt acagcccggt tggccaaatt tatttctata
    28021 cgctgaccag cacgaatccc cggtacgact tgatggagct caagtcgctc caggattggg
    28081 tgctcgagaa ggaattcaag tcggttccca acgttgtgga cgtctcgggc ttcggcggga
    28141 tggtccgcga gtaccaggtg caggtcgacc ccaataagct gatctcctac ggcctcagca
    28201 ttacccaggt ggagcaggcc ctggcggcga acaacaccaa tgcgggcggc agcttcatcg
    28261 aacacgggca gcaggcctac aacatccggg ccatcggact gatgacgtgc accgatgaca
    28321 tcggggccac gctgctcaag agccagaatg gcacgccggt gcgcgtgcgc gatgtagccg
    28381 tggtgacgca aggccctaaa atcaggctcg gccagatcgg caaagccata caccggcagg
    28441 acgggcgcgt catcgataac gatgacgtgg tggaggggat tgtactgctg cggaaaggcg
    28501 ccgagttcga cagcaccatc gaggccattc acgagaaagt gaaagacctc aacgagcacg
    28561 tgctgccgcc gggggtgaaa atcgtccctc atctcgaccg cggcaacctc gtgcactaca
    28621 caacgcagac cgtgctgcac aatctgacgg aaggcattct gctggtagtc gccattctct
    28681 ttctgttcct cggcaacatc cgcagcgctc tgattgtgac gctcaccatt ccgttctcgc
    28741 tgctgttcgc ctcgattttg ctccagctct gccacattcc tgccaatttg ctctctctcg
    28801 gagcgctcga tttcggaatg gtcgtggacg gttcggtggt gatggtcgag aacatactgc
    28861 gtcatgtgaa ccgctcgcct tcggaattgt cgttccgcga cagaatcgcc atcgccgcgc
    28921 acgaggttca gcgcccggtt ttctacgccc ggctgatcat catcacggct tatttgccga
    28981 tcttcacgct ccagcgcgtg gaaggccggc tgttccgccc gatggcgtgg accgtcgcgt
    29041 tcgcgctgct gggagcgctg atcttcgcgc tgctgctggc tcccgttctt gccagctacc
    29101 ttttccgtga gggtatgcgc gaatggcgca atcccgtcct cgcgtggctt acccgggcat
    29161 accgccgccg gctcgactgg tgcatccatc accgctggat caccaccggc gccgctgtgg
    29221 ccggtctggc cgccgttttg ctgctggctt acagtggagc ggtgggatcg gaatttctgc
    29281 cccatttgga tgaaggcgca atctgggcca ggggtactct gccctcgagc accggtccta
    29341 ccgagggcga gcgggtggtg cggcaggcgc gcctcatttt cgcgaaattt ccggaagtca
    29401 cccaggtcgt tagccagctc gggcgtcctg acgacggcac ggattccacc ggctttttca
    29461 ataccgaata cttcgtcgat ctgaagcctc atgagcagtg gcgcaaacag tttcgttcga
    29521 aagaggagtt aatcggcgcc atggaccggg agctcgaaaa aatcccgggc gtgctctgga
    29581 atttttccca gcccatcgcc gacaacatgg aagaagccat gagcggcgta aagggccagc
    29641 ttgccgtaaa actctacggc agcgatctga agcagttgga acagaaagca gatgaaatcg
    29701 tcagcgtgat gaaaaacatc cccggagtcg ccgatctcgg cgtattccgc gtcgtgggcc
    29761 agccgaacgt gaatctggtg gtggaccgcg ccaaagcgga ccgcttcggc atcaatgtcg
    29821 ccgatattca ggacgccgtc gaaacggcgg tcggcggcaa ggctgtgagc cagatcttac
    29881 aaggggagcg gcgctacgat ctggtggtcc gctaccagga gccgtatcgg cgtacagtgg
    29941 aagatatcgc cggaatccgc atcgtcgccc catccggcga acgcgtctcc ctcggccagc
    30001 tttgcgatat caaaattgaa gatggggcct ccatgattta ccgggaggct aactcacgct
    30061 acatcgccat caaatacagc gtccgcgggc gggatctggg cggcacggtg gaagccgcca
    30121 tgcgcgcggt ggctcaaaag gtcaagctgc cggaaggtta tcgcctcaac tgggccggcg
    30181 aatatgagag ccagaagcgg gccaaccggc gcctggcgat tattgtgccg atcacccttc
    30241 tggcgatttt cctcatcctt tacacggcct tcgactcctt caagtggtcg cttctgattt
    30301 tgatcaacgt ggctatggcc ccgatcggcg ggattctgac cctatatttt accaataccc
    30361 atttcagcgt ttcatcgggc ctggggttcc tggccttgtt cggcgtttcc gtggagattg
    30421 gagtcatcat ggtggaatac atcaatcaat tgcgggctcg cggccggtcg gaacgcgaag
    30481 cggcgattga gggcgccatt ctccggctca ggcccatact catgacgatg ctggtggcca
    30541 ccttgggcct gttgcccgct gccatctcca cgggcatcgg gtccgattcg cagcgcccgt
    30601 ttgcgcgcgt cattgtggga ggactggtcg ccgacctgct catcagcgtt ctgctgctgc
    30661 cgactctata cgtctggatg gcccggccgg atgataaact cccggccgcc gaaatggggg
    30721 ccgccggcga ggagtgaggg acatttctgt tcccggcgat ctccccaagc ccttgcccga
    30781 aatccacgcg gaggtctgtt cgcctcagcc cgaagtgtag tcagtgatac ttctcataaa
    30841 ccgcccatcc ccattcctct caaaggaagc gtcgtttctc taccggcgcg cctgggaagc
    30901 ggttccaaga gccctttttt gtgcaactgc tcggaaaagg actaggattg ccagccagat
    30961 agccctaaaa tggaggtctg atgcccgagc cgctgctgtt cgcgctgccc ggcagccctc
    31021 aaacctcacg ccttcggcct ttcctgctgt cgatgggaat ccatgccgcg gtgattggcc
    31081 tggcgataag cagccgcgga acgcagcctt ccacgggagc gccgccggcc tcggccgcgc
    31141 agcagacgaa ttcccaacgt aaaatcgtct ggtttaccgc ccccatgcgt ctgcccaccg
    31201 tcgcaccgcc ggaggcggat gaagagcagg cgaaccggcc caatcaaacg cagacatcgc
    31261 aaaacatagc cgcaacggcg aaacaacccg aaagccggcg tcagatggtg ctgcaagcgc
    31321 cgccggagtt gcggcttaaa agcgacgttg tctcgccgaa cgttctcgcg ttcaacccgc
    31381 cggcgcccag cccacccgcg tcgaaggtcc gctttgtacc ccccgcgaag gcggcccccg
    31441 tccgactgtg ggccctcccc gagcttaagg agccgcaagt gacggcagaa gcgcacacgc
    31501 cggctttgcc tttacagcca acccaggtca caccgccgcg gtttcaacta ccccagcggc
    31561 ccgccgccgc gcccaagcct tccgcgctcg catttagcgc tcctcagatc gccgtagcag
    31621 cagcgagtga cggactgttg gcccggctgg agagcaagct ttccatcgag cggcctaaat
    31681 tcgttctgcc cgtgcgaagc gctgcggagg ggaggtccgg gggccggagc ccgattctcg
    31741 caccagctcc tcagattgct ttgcggccgg tacaggatca aacgctggcc cggttggcgc
    31801 cggaaacagc agcatcgccg ccgcaatttg tgatgcccaa aaaaaagacg gcgccggttc
    31861 cttcagaagc gctcccactg gccggttctc ccatcccgag agttccagta cgagcagcca
    31921 tcgacaattc cttagcgcgg attcagccca gcaccgcgcc cgatatgcca aagttagcgt
    31981 tgccgcagaa acccgccgct gcgggaggcg ccgctggaca gattctcacc cccggtccgg
    32041 ttcccagcgt aagcgccttc ccgccggcgc cggcaacggt gatcgttggc ctcgacccgg
    32101 ccgccgccgc gcccccgccg ccacctggca atcgctcggc tcggttttcc gccggccccg
    32161 atgcggctgg acgtgaagac tcaccaaccc tttcggcttc ggcgaaagcc gaaattcacg
    32221 tccctaatct ttcaatccgc tccggtcctg attcgccggc ggccgccggc agcggtccgc
    32281 cgccagcggc ggatcgggcc gcctttcgcc gccagttgat ggccctcgcc acgtccacgc
    32341 agcccctttc cgacaggcta agaccttcgc ccgataccgc cggacctcct attcttcacg
    32401 gttcgaccgt ttacacgatg gccatcgata tgccgaacgt caccagctac gacgggagct
    32461 ggacgctgcg gtttaccgag ttgggagggt catcgccgga cgatgagatc accgctccct
    32521 tggcgatgca caaggtggac cccaaataca tcgcctcggc ggccgccgaa aagatcgagg
    32581 gcaatgtgct gctctacgcc gtcatccaac gggacggcca cgtgagccag gtgagactcg
    32641 tgcagggagt agatgagcga ctggattcga gcgcgatagc agcattttcc aaatgggtgt
    32701 tccagccggc caccaagaac ggccagccga ttgatctcga agccgtggta cagatccctt
    32761 tccggacggg ccctcaacgg aggaattagt ttcaccagcc gattgccttt catcaaacaa
    32821 taagaattta gattctccgg gaaccctaaa ttcggaacgc ttctgctcca cccgttcaaa
    32881 gccggggcat cgctttccga aagtaccaga atggctatat gcaaaaaaat ccagcgttcg
    32941 ggcttaagta ctagtattca gcggtcgccg caaatgcaga cctttgcatt tcagcagaac
    33001 aactaggcaa aatccgagta gacgatacgt ctggaagaac gtagtctgct gatttcttgt
    33061 ccggtttttt ctgaacacgg gtgtaccagg accgtcagtc tatagatttt gttttcgatt
    33121 tgactgacaa ttgagcttct ttacagcggc tccgtccaag ttgcgaaaaa gagcgccggt
    33181 ttcgagatcg tgttttggga tagctgcatt ggcaaaggta aaagcgctcg tcaacgattc
    33241 tgctatttca cgccgggcga ctacctcggc aggtatattc cacaactcgg gcttcctggc
    33301 agaagaccaa ggggagtgtg tcgtgccggc tatacgctgt gtaaacctgc caggcaataa
    33361 atacacggag agcgcggaag gcgcgcatcg cgcgaaagcc gacggccgat gttgccgtgt
    33421 tggcgcgcag taagccgcct gtttttgaat ttttttttgg tagcaaacat gcgtattagg
    33481 cgcatcttag ttcgggagtc catcgctaaa agggttactg gttgggcaat ggcaaagccc
    33541 cgttcggagc ttctcgggcg ctcgaggcca gagatatttt ggtccggctt caaggctgcg
    33601 tcctttgcgg gattgagcat gcgattgcga attgattcgg caatgtgcgg ctgtccaaag
    33661 agcggcataa cggcgacgcc gccttccagc cgcatggtcg gcgtgttatg cggcggccgg
    33721 aatcaaggtg taatcgagtt caggacaggt atgttcgaat gattcgactg cttctgcatt
    33781 ccggtgatcg caagttacaa agcctcctgg cggtgactct tgggtctgac tatgacgtgc
    33841 gggtcgagcc ggataagagc aaggtgaaag agatcctttc cggggagcag gctgatgttc
    33901 tgattctcga ttttgattcc gcgcaaacat ctctggaccg gctgctggcg ttcctgggag
    33961 aaatccgaga atcccgaatc cccatcgtcg tcatgacgga tgacgagaag cgctccacgg
    34021 ccatgcaatt ggtccagcac ggggtctatg atctgttccg gaaacctccc cacttgctgg
    34081 aattgaagct ggtggtccgg cgggcgcacg aacacgccca gctaaagcgg gagctcgagt
    34141 cggcgcggga aaagatcaac agcctttcca gttgcgatca gttgattgga tcgagcgccg
    34201 ccatgcggag cgtttacgat ctgatccggc gcgtcggcaa cttgaatacg aacgtgctga
    34261 tccgcggaga gagcggaacg ggcaaagagc ttgtggcccg cgccatccac aatctgagtg
    34321 atcgtgcaaa agcgccgttc atcgccgttt cctgcggggc gattcctgag accctgatcg
    34381 aggccgagct cttcggccat gacaaaggcg cttttaccgg agctacggcg gcgcgcgagg
    34441 gttacttcga gcaggcgggt gaaggcacgc tgcttctgga cgaaatcggc gagctcagcc
    34501 tgcaaaccca ggtgaagctc ctgcgagtga ttcaacagag agagttttgc cgcgtcggcg
    34561 gcaaaaaggt gctgccattg aaggcgcggc tggtctttgc aacacaccgc aacctgggca
    34621 aaatggtaga ggagggcacg tttcgccagg atctgtattt ccgcatcaac gtattcaaga
    34681 tcgagctgcc gccgctgaag gaacgcaaag aggacattcc gtcgctcgcc cggcacttcc
    34741 tgaagcagta ctgtgagtcg tgcgggaaaa gggcgctcga gcttcggccg agcgctatgc
    34801 agcggctgct tgattacgag tggcccggca acgtgcgcga gctggaaaac gtgatccagc
    34861 gagcggtgat tcttgccgag gataccagca tcggcgctga aattctgccg agcgctccgc
    34921 cgcccgcggc gacgcctgcg ctggccaccc caattgccga ttcgttcgaa gaccagctaa
    34981 aagactacaa agtcaggctg gcaagccagg ccgttatgga gtgtaacggc aacaaaacgc
    35041 tggccgcccg caaattgaac atcacacggg cttacctcca tcgtctgctt cggcagggcg
    35101 ccgaaaacga cgatgtcacg gtaaactgat gaccgccgcg gaagagctgg gggccaccct
    35161 ggaaaggccg cttgccggcc gccgcgacgc caccgccatt cgggttcgct gccttcgcgc
    35221 cgctttctca ttccccgcca tgctgtcagt gagcttctgc ctgctcatgc tgctgactgt
    35281 gcgcgggcgt tttgatgacc ccgatatgtg gtggcacttg aaggtaggcg agattatttg
    35341 gaattctcac tccattcccc gaaccgatca attttcctac accacgaatc agcattcctg
    35401 gctgccccat gaatggcttt cccagcttgc catgtatgcg gcctacagag cgggcgggta
    35461 caccgggctt tggtgctgcc tgttcggctt ggcggcgctg ctgctggttt cggtttattg
    35521 gctctgctcg ctttattccg gcaactccaa gctgtcgatg ctcggcggcc tggtcgcatg
    35581 gtttttcgga acggtcggcc tggctgtccg gccgctgatt ctcgggcatc tgctcctggt
    35641 tgtggaggtc ctcattctgc atctggcgcg ctcgcggggc cgcgcctggt tgtgggccct
    35701 gccgccgctt ttcgccctct gggtgaattg ccacggctcc tacgctctcg gcattatcgt
    35761 gctgggcata gccgtcacct gctcgttcat cagccttcgc gcaggacgct tgttctcgat
    35821 ccgccgggag ccacaggagt gccgggccct ctgtgtcgtc ttcctgatgt gcctggccgc
    35881 tctgatgatc aatccggtag gtccaaagct gctgagctac ccgctgaaca cgtttacgca
    35941 acaggccgag ggcctagctc aggtctcaga atggcagccg acggatatag ccaatccccg
    36001 cggcgccggc tttttcgcgt tagcaggcgc tttcgcgctg atttttttgc tgcggcccgt
    36061 tcaactggag ctggagggcc tgcttctgct gctggtgggt tttgcgatgg ccctccagca
    36121 cgtgcggctg cttttcgcct tcgggattct ggcggcccca attctctgcc gcctgcttgc
    36181 cgacgtctgg gatcactatg accccgacca tgacaatcgg gcagccaacg ccctgctgat
    36241 aacggcggcc atcgtcgtca tgatctgcgc attcccgaac cgttcgaacc tggaacagca
    36301 ggttcgagac cacagcccgg cggctgcggt cgaatttctc cgccaaaccg ccatctccgg
    36361 cccgatgctg aatgaatacg agtggggtgg ttatctgata tgggccttgc ccgagcagaa
    36421 ggtgttcatt gacggccggg gcgacgtttt cgattggaca ggcgtcttca gcgaatatgg
    36481 ccggtgggcg acgcttcagg aggatccgga aattctccta aacaagtatc gcattcattt
    36541 ctgcctcttg cgagccgcct cccccatggc gcgagtcctc ccatatctgc ccggctggca
    36601 aaacgtctac acggacagcc aggcagtcgt gtttgctcga aagtgaggtg ctgcgtgctt
    36661 tccccctttt gctgcccgcg aatcctgcgt ggtaggaata cgcccggcga cgcccgttcg
    36721 cgccgcattt cgattggcga agtcttccgc aaggtttttg gacccgcgcg gctggcgctc
    36781 gcgctggcgg gaccctgcct gctggcgctg gcgctttttc aacggcaagg cctcggcgat
    36841 gacggcttta agccgcccaa agacttccaa ccctccagtg atttcaggcc atccaaagac
    36901 ttccagccat ccaaggactt tggaccttca agagagttta agccgccgcg ggacttcaac
    36961 ccagcgcatg atttcaaacc ctctagagac tttactccct cgaaggactt ccaaccttcc
    37021 agagacttcc gaccgtcgag ggatttcaaa ccatcgaatg acttcaaacc cggggcgggt
    37081 ttcaaactca gccgaccgcc gtctccccag caagcgaacc cggcggcttc ggatccgcgc
    37141 gcgccggtcc ctgcggccag cactcccgcc aaacgaaagc agatttccat cacgggagcc
    37201 gacaaaacaa cggaataccc agtgggcagc cgctagccgc gtacccgaat cggccacgca
    37261 cagcagcggc ggagcggctt catacggatg cgtgacgcac cgggagcgct ttatccttcc
    37321 atggcaggat ggcgcttttg tggcggaaga tggccaaaaa aagaaccccg gggcgtctaa
    37381 ccccggggct cgctttgggg atgctttccc gattctgtgc tctaccggcg cttaagagtg
    37441 ggacggtcgt catcgtccga cttgtcgttc gtgcccgatt tcccgttctt atcctctacc
    37501 ggcactgtac tgccggaggt ggatttccgc agccgcgggc ggttggaatc ctgctcctcg
    37561 cctttgcggc gtgcgtgcaa ggaggccagc cgggctttta catcgagaaa ctcggaggtg
    37621 ttaacgacat attccggttt tgtcggcaag atctcctgaa tctccttctg ggcggcctcg
    37681 atccggtctt cggtcatcgg atgggtccgg aacgccttgg aaagagttcc cggtttcttt
    37741 ttctcaagct tttccagccg ctcgaacatc tcaaccatgc cattcggatc gtaccccgtc
    37801 ttgtacaggt actggacacc gaggtagtcc gcttcgcgct caaaaccgcg ggaaaactgc
    37861 aagaacccta aaggaatggc cagtccggcg gcctggcgga ttccatagcc ggtccatccg
    37921 cccataaaga tgaggggaat cgtactcagg ttgaccagtt cgccgcgcgt agcctgccgc
    37981 gttccgtgcc gtgccgccac atgagcgatc tcatgggcca tcgcgccagc catttctgat
    38041 tcagaatcgg ccatctgaat caggcccgag tttacaaaga agaaaccacc cggcagggac
    38101 atggcgttgg gttcgtcggt atccaacacc ttgatcgtaa agggaacctt ggcgtcggaa
    38161 ttccgtacca aattctgccc cacgcggttt atatattcag caatgatggg gtcatcgatg
    38221 atcttcgcct gccgctcgac ttcctgcgcc aattgcttac cgagtgcgat ctccttttcc
    38281 agagagtaga agtttacgcc ggagccgacg ttccgatcgc cgatcgcttc cacatcatcc
    38341 ttgcttttct ccttgacctt cttctttttt gtcttgttgt tctttggtgt ggattcgtcg
    38401 gtcgtgttcg ccgggttgtc attagcgctg gccggcttct catcgccttg cgggaaaata
    38461 ggagctgcca gtgctgctgc caagaggacc gccagggtgg tctttcgcat cgttccctcc
    38521 cttcggccgc gaaactgcca tcccagaatc gagcggctta caggaacctg tctacattat
    38581 actctgcccg aattcagggc gactgtccta ttagactgct ccgccgcgcg gaccgttact
    38641 cgttcttttg ggatgctctc tccctgcaat tcttcaggaa acagtggcgg aaccgtttgc
    38701 gcatcggatt ttttaaagac acgctcggaa accccggcct cttaacgcct gggtaggaca
    38761 gcgcgcgctc ccagctcgtc atcttctaag cttatgatac gctgagagtt gatctgcaaa
    38821 aggaaagttc ttcaacaacg catccccagc ccctccggga gattccggct ggcgcatacg
    38881 cccgcaggca ttagcggaca gcatcgaaaa ggatgcaggt ctgaggcaat cgtctggtca
    38941 gtcgggttgt gaggatagga gctctgtcga atggaatttt gggaacacta ccggcgcgaa
    39001 tttcccgtga cccgtcaatg gatttacatg aaccacgcgg ccgtgtcccc gctgccgcgc
    39061 cgggcagccc aggctatgcg gggcagcatc gaggatgtca gcttgcacgg cgtggtgcat
    39121 caccggcgct ggacggaggc ctgcgaagcc ctgcgctctt cggcggcacg cctgatcggt
    39181 tcctcggccg aagaaattgc aattgtaaag aacacttcgg aagggcttgc catcgtggcc
    39241 aacggaatcg attggcgtcc cggcgatgtt gtcgttggcg tcgaaggcga gtttcccgcc
    39301 aattactatc cctggtgcaa tctccagaaa cgcggagtca agctgcgctg ggtgcaacag
    39361 cgccgaggcg ccgtttcgct ggaagatctc gatcaggcct gctcgggcgc gcgtctgctc
    39421 gcgatcagct tcgtacagta cctgagcgga tttcgcgccg acctcgaggc gatcggcgag
    39481 atctgtgcgc ggcgggaatg cttgtttgtc gtggacgcga ttcagggtct gggagcgttt
    39541 ccggtcgatg tacggcgcgc gcgaattcac gccctttgcg ccggtagcca caagtggctg
    39601 ctggggccgg agggctgcgg gattctgtac gtaaattccg aggtgattcc gcagatcgag
    39661 ccagtggagt tcggatggac gaatgtggct cactggcagg attacgaatc gcgagacgcc
    39721 acactgcggg cgggagcagc ccgctacgag tgcggcagcc tcaacaccgc aggctgctac
    39781 ggcctgctgg ctgccctcga cttgttgctc gagattggag tcgaacgcat tggccggcgt
    39841 gtagccggat tggcgcagaa aatcgcctgc ggcttgtcca gcgccggtta tgagatcctc
    39901 gggcgattcg agcctgcttc gttgtccggc atcgtcagct tcaagaaacc cggcgccgac
    39961 gccgcccgct tgacccaaca actggccgcg atgcggatta tcgttgcgcc gcggtcgggc
    40021 tgggtccgtg catctccgca tttttacatt tccgatgcgg aagttgagga gttggtggaa
    40081 acactgaaga cgttatgagg cgccaccgcc ggggctgctc attaagcagg gctgtgaccg
    40141 atctaggtat tgcgcgcaag tgcgggggac agggctcaca ctgaaagggc gctgacgccg
    40201 cgggtatact cggcgattac ttcggcggcg gggccgtcgc gcttcacgcg gcccatttcg
    40261 agccatacgg cccgccggca aagactttcg atggtttggg cggaatgcga aacaaagagt
    40321 agcgtcttgc cctggcgtcg aaattccaag attcgctcca tgcattttcg ctggaaatcc
    40381 tgatcgccga cggcaagcac ctcgtcgagc acgagaatat ccgggtcgag gtgaatcgcg
    40441 acggaaaagc ccagtcgagc catcataccg ctcgaatagg tgcgcagcgg ctcgtcgatg
    40501 aaatcggcga tcccggcgaa atcaataatg ggatctatcc gcttgagcac catggcgcgc
    40561 gtatagccca gcagggcggc gttgaggtgg atgttttcgc gtccggtcag gtcgggatga
    40621 aagccggagc cgagttcgag cagcgccgcg gcgcgcccgc gctggatcac aacaccctcc
    40681 gtaggcgcgg aaacacccac cgcgatgctg agcagagtgc tcttgcccgc gccgttggcc
    40741 ccaattatgc cgaccgtttc gccggcctca atgcggagat ctacattccg cagcgcccag
    40801 aaggaaacgc ggccgcgccg gatgcggctc gccacgcgct ccgcgagcaa ctgccgcttg
    40861 cgatgcagaa agtagcgctt cccgacattc tgaagctcga tcagagtcat tttcagccgg
    40921 cggctttcac agctttagag gtagtcggca aactcctttt tcatgcggct gaaaagcaat
    40981 aagcccgcag ccagggcggc gaaggaaacc agcgtcagct tctccagcag cgaaaacggg
    41041 ggagccgtgg cgttgagtag aatcaggcgg cacgcgtgca cgacggcggc gaccggattc
    41101 agctcgtaga gcaaagtgta gcgcaccggc acctggccga tgtcgtagaa aatcggcacc
    41161 atccagaata gaaccatgtt ggctgactcg acgacgtagc gcgtgtctcg gtaatgcaca
    41221 tccagggcgg agaaagccag ggccatcccg cagacaaaga tgatttccag cccgaaaatt
    41281 accggcagcc aaagccaatg ccggttcagg ccgcgcccga acaacagcag cagggccagc
    41341 agaatgacga cctggatcaa gaaatgaacc acgtttccga gcacaaccga gatcggaatg
    41401 acctcgcgat gaaaacgcac cttcttcacc agcgcggcgt tgtcgaggat cgagttggtt
    41461 cccgtcgccc aggccagcgt gaaaaaattg aaagggacca agccgatcag caggaacaac
    41521 gggtagttgt ggatcggctg cggaaacact ttggtgaaaa cgtaggtcag caccgtcacc
    41581 atgatgattg gattgagcag cgaccagaaa atgccgaggg acatgttccg gtaacggatc
    41641 ttgaaatcct tgctgatcag gttgtagagg atataaagat ggtcgtgcgc cgcaggggtc
    41701 attctggatg caagcttaca ttttaccacc ggggggcgcc ggagaaagag gtcttcaaac
    41761 cggctgattt tggagtcgaa acgccatccg ccttccagac aagccgttta tcgcagccga
    41821 agacttgatc cgctttgcag ctcctggcgc cgttcgccgg ggcaggatcg cccgccctcc
    41881 ggtttgcgcc gcgctgagag cttgttggcg cgctagccgc aatcccgggc gttccgcgcc
    41941 cggcggcagg tcccggcaag ttatggtttc agatcagcga tcgtcgcacc ccagccgccc
    42001 gcctcttccg gagcgtcggc gaaccgggcc acaaatggag tgcgcgcgag aaccgatcgg
    42061 accatttcgc gctgcacgcc gattcctttg ccgtggatga tgcgcacgca ccggaatccc
    42121 agacggtgag cttcctccaa atacgcctcc acggcagcgc gaacttcccg cggcggcacg
    42181 gtgtggagat cgaagacgtc ggccacaggt atgcgcacgg gttccggaaa cgggctgtcc
    42241 atgcaagtgc ggttttttcc tttccgcccg gagcgccgat tccgctcgaa aacggccgcc
    42301 ggtctgggcc ttctacttca ttcgagtcgt ctcatccgcc gacgtaacgc gtataaagac
    42361 tccgcgcaac gctgatgtcg ctggtaccct tgacgatagc gcggccatcc gggaacacgg
    42421 tcatttcgta tggcgggcaa agaaaacgca gcgcatgctc gttggcacgc actgtgccca
    42481 gccggcgcag cgactcgccc agcgaatgga gatcgacagg cccggcggga gcgcgtatct
    42541 gcacagcatt gcggccgcac agactgatcg gcggccggtc cgggccattg agataacgaa
    42601 attcggatcg cgcgcaggtc aagcactccg gatcccgctc ccgagtggaa atcgagcgcc
    42661 actggttgct ccagatgtcg atcgagaaga gccgcggctc gacgcttcgt tcgcttcccg
    42721 acagaatctt gagagcatcg gcgacttgca agcaagccac cagagccgtg atcgcattca
    42781 agacgccggc tgtgtcgcag gtcggctgat tgcccgaggg cggcgacgga aacaggcagc
    42841 tcaagcacgc cgagacgcgg ggtaaaacgg gcatggtgaa gccggagctg ccgaccgccg
    42901 cgccgtagat ccaggaaacg ccgtgcttca cagcgacgtc gtttacaagg taacgagcct
    42961 cgaaattgtc cgcgccatcc agaatgacat ttgcaggcaa aagcagttct tcggcgttgc
    43021 gggcggtgag atccgccacc acgggatgca ctcgcacttc tgagttgatg gcggccagct
    43081 tttccgccgc cgccacggcc ttcggcagac ctctggcggc gtccgcttca tcgaaaagcc
    43141 actggcgctg gaggttgctg ggctcgatgt aatcgcggtc gatgatgacg atgtcgccaa
    43201 caccggccct ggcaagagcg caggcctgga cgctgcccag agcgccgcag cccacaatca
    43261 cggcgcggga agcgaacagc ttctcctgtc cctgctctcc caaaggggga aaaagaatct
    43321 ggcgcgagta cttctcgcgt gccaaggggc ttatcatggc gggtgctgag ccgcttccgt
    43381 acccagtata atttagggca gcggacagtg ggcagtgggc agcagacctc ggctatggct
    43441 taagccggcg ggccgccgcg agcgtccagc gtctactgcc tactgccaac tgaatgttcc
    43501 gctccgcctt tatcgcggtg gctttgtgtc tggcgccggt ctggagcgtc tcgggcgcag
    43561 acaatccatt cgaggggctg cctgtcaccc agattttgtt tattcccgcc gcgcagccga
    43621 tctcccccgc ccggcttaag gaattaatag ctctgcggga aggcgcgccg ctcgacggag
    43681 aaagcttgag caagagcatc aaacaaatgt tcgccaccgg acgctatagc gacatccagg
    43741 tggatgccga gcgctccgcg aacggggtgc gcatcagctt tatcacttcg cagagctggt
    43801 ttgtgggtgc ggtgagagtg ttgaacgtcg aacagccgcc caccgcggcg cagctcgtcg
    43861 cagccacgaa tctcgtgctg ggtgagacat actcggatga aaaaatgaag gccgccaccg
    43921 aagcgctgcg cgcgctgctc gccgaaaatg gcctgtacca agcaggcatt cgcgccgaga
    43981 ccaagggcca tccggacacc cagcagctcg atatcacttt tctggtgacg cccggacccc
    44041 gggccgcctt cggcgacatt cgcctcccgg gccgggccga cgtcacagcc gaacaggttc
    44101 gccgcatcac tcgatggtcc aaaggccggc ggttcaccca gcccgccgtg gaacggggga
    44161 tggcgcgatt gcagaggtac tatcagcgca ccgatcacct catggctacc gccagagtag
    44221 cggagcggga atacgtgccc ggcgccggcc tggtgaattt aacaatcgaa atcgatcccg
    44281 ggccgcgcgt ggaagtcgct ctttcgggcg ccaagctctc gcagaaggaa ttacgccgcc
    44341 tggtgcctat ctacgaagag ggttctgtgg accgcgatct gctcgcggaa ggagcccgca
    44401 atctgcgcga ttacttccag acccagggtt actttgaggc caaggtggat tacgacgaac
    44461 atccggaaga aaacggcgtt gtgctggtgg agtttcaagc cacgctgggg gaaccgcatg
    44521 aatttgtaag actggaaatt gacggcgagc gctactttga tgaagagacg atccgggagc
    44581 gtatgctgct gcaacccaag ggcctgcaca tgcggcgcgg gcgcttcagc cagaatctgc
    44641 tgagcgccga catcgccgcc attcaagacc tctaccggtc gaatggcttc ctgtccgcca
    44701 aagtagaacc gcgcgtcgcc gatgactacc agggacggaa aggcgagatc gccgtgtttt
    44761 tacgaatcga ggaagggccg cagactctgg tttcccggct gacaatcaca ggcaaccagg
    44821 ctatttcgac ccgggagctg ctcaatcgcg cgtcctctct gccgggccag ccgttcagca
    44881 caaccaatgt cgcgggcgat cgcgatcaga ttctcgactt gtatttcgga cagggttttg
    44941 ccgatgcgac cctggaatgg agttccactc cggccgccga gcccaaccgg gtgaatctcg
    45001 agtacacaat tcacgagggc cggcggcagt tcgtgaacaa ggtcatcgta gagggttacg
    45061 aaaatacccg cccctacatt atcgaccggc agatccagat tgagaacggc cagccccttt
    45121 ctcaggaggc catgctggag agccagcgcc gcctttacga tctcggaatt ttctccaaag
    45181 tggatatggc cattcagaac cccgagggag aagaacaata ccgcaatgtt ttgtttgaaa
    45241 cgctcgaggc gcgacgctgg accgtcggcg tgggcggcgg cgctgagatc ggccgcatcg
    45301 gcggcagcac aacgagcctg gacgcgccgg ctggcgccaa cggcttcagc ccgcgcgtgt
    45361 cgctggaagt caaccgcctc aacatgttcg gccgggccaa cactctcagc ttccaatccc
    45421 gcgtgtcaaa cttcgacaag cgcgtcctcg cgcggtacac cgctccgcaa tggcgcggga
    45481 aggaacgcct cactttgacc tacaactctc tgttcgacca gtcggtcgat atacggacct
    45541 tctcggccac ccgcctggaa ggcggactac aattgcagca tcgcatcagc aagacgacga
    45601 gcctgtttta ccgctacacc taccgccacg tgaccgtgga tgctgcgacg ctcaagatca
    45661 acccgctgct gatccctctg ttttcgcagc cggtgcgggt ggggctgctt tccgcctctt
    45721 tattccagga ccggcgcgac gacccggtgg acgccacgcg aggcatgtac aattctatag
    45781 acctcggctc ggccagctac tacaccggtt cggccgacac attcgcccga ttgctgatgc
    45841 agaattcgac gtattatccg ctgaccaagc ggctcgtcct ggcgcgcacc acgcagttcg
    45901 gcgcgatggt tccttatggc cgcctgcgcc gcgtcgagac ttccaccgaa tccggcgccg
    45961 cccaggtgac gtttacgcgg gaaattcccc tgccggagcg ttttttcgcg ggcggcccga
    46021 actcgattcg ggcgtttcca atcaatcagg caggtccgcg cgatctcgtc accggctttc
    46081 ccgtgggcgg gaccggtttg ttctttaaca gcgtcgaatt gagattcccc gtccggggac
    46141 ccaatttggg cggcgtcctg tttcatgata tgggcaacgt gtactccaaa ccgcagagca
    46201 tcagctttcg cgtgacccag cggaagcccg aatttgacgc caacggcaat atcgtcagcg
    46261 aggatttcga ttacatggtg catgccgtag gtctgggagt gcgctaccgg actcctatcg
    46321 ggcccgtgcg gctggatgtc gcctacggat tgaacccgcc ccgcttcttc ggcttcaaag
    46381 gcagccagga ggatctgctt ttcgggcgcg gcacgaaaac gaaccagcgt atttcgcatt
    46441 tccagtttca tttttctcta gggcagacct tctgatgtcg gctcgattgc tcagtcgcgc
    46501 catttcaagt tttcttctgg ctgccgtgtc tcttccgcct tccgccgcgc gcgcggaaat
    46561 tattgatcga atcgttgccg ttgtcggccg ccaggtggtt accgacagcg atgtgcagcg
    46621 ggaagcccgg ctggaagcct acttcgatgg gcagcctccg ccgggttccc tggctcccca
    46681 gactccgcaa tatcggcagg tgctggagcg gctgatccgg cagcaactga tccaatatga
    46741 aatggaccaa acccggtttc cgtcggttga cgccgcgcag gccaaaaagc aattgcaggc
    46801 tatgaacccc gtacagggga agccgggcga ctataacctc aaggagcagg atttaatcga
    46861 ttacgcgcgc cgcctggcca atgtggcccg atttttagag cttcgctttg ggaccctgga
    46921 tgccgggtgg aagccgcctg cgatcccgga ggagcagctc gaaacatact atcgcgatgt
    46981 ttttctgccc tcgctggctc gcaaagacaa cagctcgctc ccctcgctca acgatgtgcg
    47041 cggagaaatc gagcaaaccc tgagacgtga aaccgagatc gacgcgtggc tgaaagagct
    47101 ccggtcccgt gtcggtgtgc gaattatgga gcccagaaaa cgatgaagct ggctgttcgc
    47161 atcggcgtgg ccctgcttgc gctgattttg gcgttgatcg gcggcgggct gcttgcgctc
    47221 cgcacgggct ggttccggga aaaattgcgg caaagggtgg ttgccgaact ggaaaacgac
    47281 acggggggac gcgtggagat cgcgcggctc gacttcgatc cgcaaagatg gcatgcgcgc
    47341 cttgaagggc tgaccattca cggcctcgag gggctggatc agaagccgct ttttcacgcc
    47401 cgcgctctgg aaatcgatct gaaggtggtc tcgttcttgg aaaagagagt gaatatcgcc
    47461 ggattgcggc tcgaggggcc ggagattcat atccaagtgt ctcccggcgg atcaaccaat
    47521 atcccggcgc cccggggatc tgccgcgggc cggccggtcg cggaggagtt gctcaatctg
    47581 gccgtaggcc gcttcgaatt ggagagcggc tctctgtggt ggaacgacag gaggtatgcg
    47641 ctggagttcg ccgcctcagg cctgcgcgcc cagctgcggt ttgaacaggc cggccgctac
    47701 attgggcaga tcgaagcgcg ggaggcgcag atcgctctgc caccaagaat ccaggaaatc
    47761 aatcatctgg ccgccaattt ccagatttct cgcgatcaga ttgaaatcgg cagcgtctca
    47821 ggcaaaactg cattatccga gtttcgagcg cacgggtcga tcaccgattt ctctcaaccg
    47881 cattcgcagt ttgattacga tgtggccgcg gacgcgcggg aggctgccgc tatgcttgcg
    47941 ctgccagaac tgaaaggggg ccgcgtgcag cttaagggct ccgtcgaggc gcgtggggcg
    48001 cgctggagcg catccggaaa gctcacgggc caacagcttg cagccgctgg ccgggagttc
    48061 aacgtgcgca atgtgagcct cagcggcaac tatcgcctcg acccccgcgg catatcggtc
    48121 gagccgttcg cggcgcggct cctgggaggc gaatggaaag gccgaattca ggctgactac
    48181 aaagggcagg cgcgggtcga aggcagagcg gagggcatcc gcctggaagc tgcggcagcc
    48241 gcagtgagca gctcgagttt cccgctgagc aagctgggct gggcctcggt gatcagcggg
    48301 acgatcaaag cccgcggggt atggccctcg cttttcagga gtgtgaatgt tcgcgccgag
    48361 ttgactctgg cggcgccagc ggacccgggc ggccgcacgc cagtacaagg tacgctgcga
    48421 gcgtcctatc aggcgccgac actggacctc caattggacc agatgcagtt ggatatgcct
    48481 gccagttcgt tgaccgcctc cgggcaactt ggccgcgcca gcagcgtcca atttcgagtg
    48541 gcgacaacac ggcttgagga attgaccgcc gccgcttcgg cggtcttcgg agagaaggtg
    48601 gaaccacccg tgaagcttga agggcgtgcc gtatttgtag gcagggcctc cggcgccatg
    48661 cggcagcccc agatcgacgc agccgtaaac gtttcacgat tcgtccagga aggaagagag
    48721 tgggattcgt ttacaggccg cgtgcaatgg tcgcgcacgc gcctccgcct gactggaggc
    48781 cgcctgacca gaggcgcgac cgcggtgtat gcgaacctga tggtgggact cgaagacggc
    48841 cgaatcgagg cgaactctcc agtgcagggc gatttttcga tccgtgacac gcaaatacag
    48901 gacctcgccg cgctcgcccg gcaaaaaacg ccgttccagg gcgcactgac agcctcgctc
    48961 agagtcgatg ggacccggca gaatctgcgg gcctcgggag acatagagat ccggcgcggc
    49021 gccgcatggg gggagccttt cggcgatttt cgggcggcag tggttttgga accaacgcaa
    49081 gttcagttaa gccgttttca ggttgccaag ggaagcagcg ccatcagcgg ctccggcatg
    49141 ttcgaccacc agcggagcct gtttcgtttc gacgtacgct cggagaaact ggctcttgcc
    49201 gattggcagg tgctggagaa aaccggcaag agggtgagcg gcagcggcgc cttccacctt
    49261 tcaggatcgg gccgcctccg cgccggagag acctttcccg aagatatggc cttgagcggt
    49321 gatctccggc tgcaaaagct ctcggtggat ggccggccct tcggagcgtt ggcagccact
    49381 gtccgggaac aggggaacaa gctccacgtg caggcggagt cggatttcac cggagctcaa
    49441 atcagaatgt cggccgacgt cgttccgcgc gatcggttcc cgatcgacgg ccgtatcgag
    49501 tttcgcaacg cgaacctctc cgccgcattc caggcggtgc ggccggtccg tcttcccgaa
    49561 aaagtgccgg ctgatgggga tatcacaatt tcgggcgagg cccgttcact cgcgtctctc
    49621 gtcctggcgg ggacaatcac aaggctcgaa gtgccgttgc cccaaaccgc tgccgcggcg
    49681 cgcattcaat ctctccacag cccggagccg gtgaagtggc gtctggccgg ccgccggctg
    49741 gttgtggaag cagcccacct ggtcggcgag ggcacagacc ttcgtttcag cggaacggcc
    49801 agtctggatg gtccgatcaa cttcggggcc aaaggcagtg tgaatctggc ggtcctcccg
    49861 gggcttgggc cgaacgtgga cgtccgcggc gcgtctgtta tcgacgcagc ggtgaccggc
    49921 acactgcgcc agccggatat tcgtggcagc cttgaaatcc gcgatgcctc ggcaggctcg
    49981 gagaatctgc cggtcagcct cagcaacgcc aacgggacca tttccttctc ccgatatagc
    50041 gcgacaatcc agaagcttac agccgaagct ggcggcggcc aaatcagctt ttccggcgat
    50101 gccacttttc tgcccgccca gcccttcacc tatcgcgtaa gcgccgaggc gcaccaggtg
    50161 cgcctgcgat acccgaaggg tcttaccacg gtgctggagg cggccttaac cttcgccggc
    50221 acagatcagc agagcctttt gagcggcgac atcaaggtga ctcgcttcgg caccagggcg
    50281 tcgatggatg tgggtgcgct cctggtagcc ctgcggcagc cgacgcgcac gcccagccgg
    50341 aacggctggc tgcaaggcgc gcaactgaac gtggcgatca cgaccgcgcc gaatatccgc
    50401 ttcgagactt cgctcgctcg taatttccaa gctgacgcca acctgcgctt gcgaggcacg
    50461 gtcctgaacc cagctctctt cgggcggatc aatatcatcc aaggcgactt cgagtttcag
    50521 ggaacccgtt atgcgattaa ccggggagaa gtgactttct cgaatccgtt ccgcatcgaa
    50581 ccgattctca atatggatct ggagacccgt gtcagcagct acgacatagt gttgacgctg
    50641 tccgggccct tacaaaagct gaacgtcagc taccgctccg accccccgct tcctttcagc
    50701 gaagttatta ctcttttggc tgtcggacga gctccctcca cggagggaac cctggccgcc
    50761 cagcagaatg cgcaagccca atcgctgacg caactcggcg ccagcaccgt gattggccaa
    50821 gccattgaga agcccgtaac gggcggtttg caacgctttt tcggcgtcag ccggctgaag
    50881 gtcgatccgg aattgctcgg cccggagggg aaccccaacg ctcgagtcac cttggagcag
    50941 caagtcagca gagacgtaac gttcacctat atctacaacc tggcgtcggc ccaggaacag
    51001 atcgtgcgca tccaatgggc gctcagttcg cagctctctg tgatcgcggt tcgcgaccag
    51061 aatggcgtgt tcggcgtcga tttcttgtat aggaggagat accgctagtg cccgattggg
    51121 agcgattgcg cgaagaaatg gtggaaaagc aactacgccg ccggggcatt cgcgacttcg
    51181 cagtactcga ggccatgcgg cgggtgcccc gccacgaatt tgttcccggg cccgccgcga
    51241 cccaggctta cagcgatgag ccgctgccga taggcgccgg ccaaaccatc tcccagccgt
    51301 atatggtagc cgccatggca gaggcgctcc atctgaatca ctgtgagcgg gtgctggaga
    51361 ttggcgccgg atgcggttac cacgccgccg tgctggcgga actggcggcc gaggttttca
    51421 ccattgaacg cgagccggag ctggcccggc ttgcccgcga aaatctttcg cggctgggct
    51481 acagccgtgt cgtagtcatc gagggcgatg ggtctttggg gtatcctgat gcagcgccct
    51541 atcaggcgat ctcggtggcg gccggagctc ccggagtccc cgcccggttg ttggagcaac
    51601 tggaagaaaa cacgggccgg ctggtgatcc ccgtgggctc gctggatgac caggagctcc
    51661 gccttattca caaatttacc gatgggcttc agactcgagt cgtgaactat tgccggttcg
    51721 taccactgcg gggcatcatg gcctggcgtc tcaagggaac gccgtgaaac acatcatcgt
    51781 cggcacggcg gggcacatcg accacggcaa gacagccctg gttcgggcgc ttaccggaac
    51841 cgacacggac cgcctcgaag aagaaaaaag gcgaggcatc tccattgata tcggcttcgc
    51901 cagcctaagg ctggccagcg gcggggagca tcacgccgct ttcgaccgcg gcgccaaaaa
    51961 cggggccgag atcgagctgg ggtttgtgga tgtgcccggc cacgagcggt tcgtgaagaa
    52021 tatggtcgcc ggcattgccg gcatcgattt ggtgctcttt gttgtggcgg cggatgaatc
    52081 catcaaaccg cagactcggg aacatttcga catttgccgg ctgctcggca tccgccatgg
    52141 cattgtcgtg ctcaccaaaa ccgatctggc cgaccgcgag ctgatcgagc tcgtcaagct
    52201 ggaagtggag gaatttacca ggggctcctt tctggaaggc gcgccgatcg tccccgtgag
    52261 ctcgcgcacg ggagagggaa tcgagcgttt gaaatccgag ttgcacagga tgggggaggc
    52321 tgtgcagccg aaagacgccc ggcatcactt tcgaatgccc atcgaccggg tattcgtaat
    52381 taagggcttt ggcacagtgg tgaccggcac gctcatttca gggacggtgg agagggaagc
    52441 cgaagtggaa atctatccgg tgggcctgcg agcccgggtg cgcggcatcg aagtgcataa
    52501 ccagccggtg gaccgcgcct gcgcgggcca gcgcacggcg ctcaatctgg caggcgtaga
    52561 gagcagcgaa ctcgcgcggg gaatggtggt tgcggccgcg ggaaggttcc ggccgacgca
    52621 gcggatcgat tgcgtagtgg atcttcttgc ctccgcccgt cctttaaagc accgtgcccc
    52681 cgtccacttc catacgggta cggccgaggt ggaggcggaa ttttatctgt ttgggaaatg
    52741 cgctcagctt gctcccggcg tgcgcgcttt cgcgcaaatc cggctaggcg cgccggttct
    52801 ggcgcttcct ggagaccgct ttatcatccg ccagttctcg ccggtcgtca ccatcggcgg
    52861 cggcgttgtt ctggacaacc tggcggcgaa gcatcgcagt ggagagccgg ttgaggaccg
    52921 cctcaggctg ctcgtagagg gggggccgtg ccgcacgttg gaatttattt tggctgaggc
    52981 caactcgggt ctgggcctgc acgagatcat cacgcgcaca ggctggctcg agagcgaagt
    53041 ggaagcggcc gcggcggaag ccgtacgtgt tggactcgcc gaacgcatct cggaagcgcc
    53101 tctctggctg gtccaccgcg atcaattcgc ggctgccgcg ggccgtactg tagccatgct
    53161 cgaacgcttc cacgccgaaa atcccctcgt ccccgggccg gggaaagaac ttctccgggc
    53221 gagtcttttt cccggcgctc cgccggcttt cctcgacgcg ttgctggagc ggttgcagcg
    53281 cgaccgccag gtggtgatcg aaggcgatgt ggttcgcctc cgctcgcacc agatcgtgct
    53341 gaatgaagag gaaagccagg cgcgtgaaaa aatcctggca gcattcgagc aggccgggct
    53401 gactactccc ggagtggacg acgtgctggg aaaagtaccc gtggagcgac cgcgcgcgca
    53461 gaaaattttg caaatgcttc tccgcgagcg ccttttgata cgggtggcgg agggcttggt
    53521 atttcatgcc agcgctatcg agagcctgaa agaaatgctc gcccgccaga aaacgaaatc
    53581 agaccgctta acggtgcctc agttcaaaga gctcaccggc atcagccgca agtacgccat
    53641 tcctttgctt gaatttctgg accgagagaa aatcacccgc cgatccggcg acgaaagaat
    53701 tattctttag actccttcgc cctcccagga tttctgcgga atccatcatg aaagatcccc
    53761 ttgcacgccg agtcgccgcg cgtgcaaagg cctacaattc tagcgaaagt aaatgcttat
    53821 gagcactccg ctttcccagc catagcgcct tgccttctgg ctgcggacgc accgccgcgg
    53881 ccccagaatg cctagccccg gaggcctttt ggcacgcagg cccgcgcaca tagccttctg
    53941 atcaccgcta ttttcgcaat ggcagcgtca gtccgccgtc cggagactaa tttccagaaa
    54001 ggcttattcg gcggcttctt tacggcgctg ctcaaagatc cttcgggctt cggcgaattg
    54061 ggccagcaca tgttggcgct ctttatccga acgccacccg gagtgctgcc cagcctggcg
    54121 ggtaatatca tcgatccatc ggatgaagta ttgcgcgtcc tccgccgagc ggatcggccg
    54181 tccgccgcaa atcacataaa tcgggctcgt ttctgcataa gggaacagat catcaatggg
    54241 tctttggggc tgaccgccat gggcgcgcag cgtgtaccac ccgctgtgcg agacgcgtac
    54301 agcggtgttg atttccgcgg ccgatccttt accatcgagc ggcattgatt gaatgacatt
    54361 tccattgttg atgatttcga gcttctccag cggaacaatg gactcgactt tcccactgaa
    54421 ctgcaacacg gcgccctcgg caggcagaga gatttcgtcg cccggcatcc ggccattcac
    54481 atgaaaatcc agcagtggcc cattggtaaa gaacgttcgg ccttcccgaa aggcatccac
    54541 ccaagccgaa taatcgagct tcggacctaa gtaggcatac gttcggtcag aacccatgat
    54601 cggcgtccgg tgcaggctga ggatcgagtc ttcgccgccc gtgcctgaga gcctgaatcc
    54661 gcaattcagt gcgcggtgcc acaccgcagt agtatttttc tggtaggcgg ctccggtcag
    54721 cacttccagg taatccgtca cgcccagcgc cagatccaca ggaaagcccc gggacgcggc
    54781 atagcccgct ttttcggggt ccctcgtcca gggatgaacg tagccggtaa gcgctccctg
    54841 ggcttttgcg atctgaaaga tgtcggtatt gctcggatac aggctttcga tcgccgtgcc
    54901 ctcatagccg gtggtgaacg gcgaaatgag atgcttggcc aaattcacga aagacacatg
    54961 gccgtaaaag ggcggccggt actcttcgtt gaaaaatagc aggcgctgcg gcgtcgagcg
    55021 gcgatccaca ctgccagtga agtattgata atcgaaaatg cgattgtctt tgttgcagat
    55081 cttgtcttcg attacgtcga tatcctcggc ggacgccatc agcatcatgt tctcgggggt
    55141 gttgagcaaa ttgccgccgt agttcatgtg gacgtgattg cttccgctgt accatccctg
    55201 cgatttccag ttcgtcatcc gcgatagcgc aagatcgacg tgcctgtatt cgcccggccg
    55261 aacctcaatg ctttggctgg ccgggcggta ttcgaagcct ttcacggcct cgatagcgag
    55321 cctgcccggc ggcacttgca tcacactgcg cccggcagtg tgaaaaaaat gttcgccgag
    55381 tcgctcaccc acacgctggt aagcatcggg cggtgcgtag gtcttgccgt cggcggcccg
    55441 ggcataaacc cgcgcggcgg caagctgccc ggaggacgca tctttcaccg tgatctccac
    55501 cgttcccatc ggccgccggt acactttgcg ccggatttcg atttttcttt cctcgccgcc
    55561 gaaagtcttg aggagccgca gctccgacag gccgttctgg ttcgatacgt aaacgatcca
    55621 ttcgccatcc ggcgaccagc gcggctcaaa gtggtcccag gcgccaaaag tcaattgata
    55681 tggctcgccc ccctctacag gaaggacata caggttggtg aactgggagc cgcgatgtga
    55741 gctatagaga atgcgcttgc cgtcgggaga ccattgcggg cgcgtgcgat agagcgtctc
    55801 ctcacgcaag atcaagcgcg cgccggccat cgcgtctggc ttcacaggca tgcgccagat
    55861 gccgcccgag cccagcggaa tgccgcggtt cgaaatggcg atgatctcct tgccatcggg
    55921 agaccaagtg ggctcgatgt gggaatcgta atcgctgaaa tacaggcgcg gccggccgaa
    55981 gcggtgatcg gcggtgattt gcacaggggc gcctggcgcg ccgttgttta cgggcatcac
    56041 gtaaatgttg aaccagccgt tcggcgcggt ggacacatag gcgagttgtt tgccgtccgg
    56101 ggaccatgcg gggtccacat tcaccgagtc gccgcgcgtc aaaacggtgc tctcgccggt
    56161 gcgcacgttt agcagcatga ggtggatggt cttgccgtcg tcctctgccg tgtaggcgat
    56221 ccatcgtccg tccggcgacc acacaggcga agagtcgtag gtgcggttcg ccgtcagttc
    56281 gtaagcggtt gtgtccccaa cgcggatctt ccagatggat ccggacatgg cgaacgcaat
    56341 ctctttaccg tcgggagacc acgccggacg ccagggagtg ctcgatgcgg caggaaggta
    56401 gtaattgtgc atgtaattgc cgcctgtcct ggcggcgcgg aacgcgcggg cgtcgtcctg
    56461 cgccggcgca gccccggccg tcagcatgag gagaatcgca atccaaaagg caaagccctg
    56521 ccccaagacg gctgccggcg aacgccatcg agaacgcctg tgacccgccg cggcctcggt
    56581 aaacatggcg cagccggcga ggtcgggctt tcctcccctc gggccgcgca gccaggactt
    56641 tccaccggcc gcgcccgcgt ccggccgaga tgcaaacaag cgccctttcc acatagcatt
    56701 cctctccaat gccgcatagt atagcggttc gcacaaaaac gaacgggggc gcagcgccgt
    56761 gccaagccgc ctgtgacggc aaccgcgccg gtcagtataa ttgggcttgc gacaaggtcg
    56821 tgccgtcgca gcgaataggg tgatatcgta gggagttgaa aaaggccggg aattctgcgc
    56881 gagaaggtaa tcagctatgc agatcatccg cagtcaagaa ccttatgatg tggtggtagt
    56941 cggctccgga gccggaggag gcatggccgc caaggtgctc actgatggcg gcctgaaggt
    57001 cgccatgctc gaagccggcg ggccgctgaa tccggagaag gattattacg agcacaagtg
    57061 gccgtatgag ttcgaccacc gcggattgcg gctgcccaaa gggcgttccg gtttcgcggc
    57121 tcccaatctg ttcaccgaga tcgacggcga gccttacaca gtggccccgg gcgaggaatt
    57181 tcactggttt cggtcgcgga tcgtcggcgg ccgaaccaat cactggggcc gcatttcgct
    57241 gcgctacgcg ccttatgatt tcaagcccta ttccaccgac ggttacggct tcgattggcc
    57301 cattacctat gaagacgtcg ccccctacta cgataaggtc gagcgctata tcggcgtctt
    57361 cggaaccaag gaaggaattc cgaccgcccc agacggaatt ttccagccgc cgccaaagcc
    57421 gcgctgcacc gaagttctta tcaagcgcgc ctgcgacaag ctcaagatcc tttgcattcc
    57481 ctcgcggctg gccattttga cgagaccgct gaacggcaga cccgcctgcc attattgcca
    57541 gcaatgcggg cgcgggtgcg tcacggcagc caacttttct tcgagccaag tcttgatttt
    57601 tccggcccaa aagtccggcc ggctcacctt gatcaccaac gcgatggcgc gagaagtggt
    57661 cacgaaccgc gaaggaaagg tcacggccgt ttcctatgtt gacacgaaca cgcgctcgga
    57721 aaagcaggtg cgctgccgcg ccgtcgtcct ggctgcgagc gcttgtgaat cagcccgcct
    57781 actcctgaac tcaaaatcct cgctgttccc caacggtctg gcgaacggga gcggaaccgt
    57841 cggccggtac ttgaccgata cggtcggcgc tcacgtgagc ggaatcctgc cgcagctcgc
    57901 cggcctgcct tcccataatc atgacggcgt cgggggcatg catctttata cgccgtggtg
    57961 gctttatgaa aaggatcgca agccggattt tccccgcggc tatcacatcg aatacagcgg
    58021 cggcccgcgc atgccggcag tcggaatgtt ccggggcgcg gccgcccggc tggaaggcta
    58081 cggcgccgac ctgaagcgc
//
LOCUS       DASREK010000249         7087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2267459, whole genome shotgun sequence.
ACCESSION   DASREK010000249 DASREK010000000
VERSION     DASREK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267459"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFA54_13490"
     CDS             complement(<1..334)
                     /locus_tag="VFA54_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51874.1"
                     /translation="MRRTALTVLLLGCAAGWAQNQALPPAPRPLFWRPLGGHAIELGL
                     AGPASAPVFDVAFSSDGTQLIVLTSRGEVWTTSDFGETWTHSAESFATVAQFRAAKAS
                     GDIPPPAQD"
     gene            561..1358
                     /locus_tag="VFA54_13495"
     CDS             561..1358
                     /locus_tag="VFA54_13495"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HZS51875.1"
                     /translation="MLVSALLNNRKAWLAVSILAALAALPAPARADGDPGTAAKVVQA
                     EGQVSVLRNSELWALFAGNSVRVGETIVTGADGYARLEVTDGSSFEVYPNSRVVFRSN
                     PGNLRELLEVFLGKVKVHIQTLGGRPNPYRVESPTAVISVRGTVFEVAVESDNVTWVG
                     VDEGLVMVEHRLLPGKAVPLAPGESLQIFPNASLAAAGIDKARLAIRIADAAREALYV
                     LRTVGVGGKNGGKGLPGPAPGPSPSPLPTDKQAPPPPPPPPPPPSGP"
     gene            complement(1916..2266)
                     /locus_tag="VFA54_13500"
     CDS             complement(1916..2266)
                     /locus_tag="VFA54_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZS51876.1"
                     /translation="MRIFVGNLPFSATDTDLSSLFEPFGEVQSAQIMTDRDTGKPRGF
                     GFVEMSSDDAQKAIAALNGKDFQGRSINVNEARPKADRGAGFHPGGGFGDRRGRNERG
                     GHRGGGRPPREPRW"
     gene            complement(2489..2749)
                     /locus_tag="VFA54_13505"
     CDS             complement(2489..2749)
                     /locus_tag="VFA54_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51877.1"
                     /translation="MRDPAFDMIWQKVAAFEGAEFRTSKGASFTYRYGKTYIVVNTGN
                     QSIPRTFFEKIFRRLAEGTVESSPPLQGQVFILAILQDPRLR"
     gene            2768..3952
                     /locus_tag="VFA54_13510"
     CDS             2768..3952
                     /locus_tag="VFA54_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZS51878.1"
                     /translation="MENRPRFASRTSWNLELNPLAQALEARRKSGREILDLTESNPTR
                     CGFQYDGDSPLRALTDPRAMFYEPHPRGLLPAREAVARYYAEQRAAVRPEHIVLTSGS
                     SEAYSFLFRLLAEPGDNVLVPRPGYPLLEFLSRLNDVELSSYRLMYHGNWEIDFDSLE
                     AAADERTRAVIVVNPNNPTGSIVRPSERLMIEDFCRRRGLALISDEVFLDYTFPEAAG
                     AAGTFAGTSGCLAFALNGLSKTAALPQMKLGWIAAAGPAGLAEEALDRLEVIADTYLS
                     VATPAQWALPELLAARRRIQRQILDRITGNLHCLDQLLSRQSLCARSNIEAGWSAILR
                     APGTLSDEERALELLDKDGVLVHPGHFFDFGGEGYLVVSLLPPPLVFREGISRMLARI
                     TA"
     gene            complement(4034..5896)
                     /gene="hscA"
                     /locus_tag="VFA54_13515"
     CDS             complement(4034..5896)
                     /gene="hscA"
                     /locus_tag="VFA54_13515"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HZS51879.1"
                     /translation="MTDLRERDSNTQSPEVIVGIDLGTTNSLIACMETSRPKIIAGPG
                     GERIVPSVVAMTGSGELAAGRPAAEVLLSRPDRAVYSVKRLMGKGIADVAGELHWFPF
                     RITEGSESVIRIALGDRTLTPPEISAHVLRRLRRNAEACLGVPVTKAVITVPAYFNDA
                     QRQATKDAGRIAGLDVLRLVNEPTAAALAYGLDQRKKGIIAVYDLGGGTFDISILKLQ
                     GGIFEVLATNGDTHLGGDDIDNLLVQIALEDLASEWAEDLSGDAIAVQLLRRAVMEAK
                     HALSTEKQAEIRFSWQGRHYRRWIDRPLFEKWIAGIVEKTIDPCRQAIEDAHVHIRQI
                     DEVVLVGGSTRIPLVRQAVELMFQRKPHIELNPEEVVALGAAVQAGILAGVQRDMLLL
                     DVTPLSLGIETLGGVVSTLIHRNSTIPASAAEVFTTGVDGQTNVVIHVVQGEREMAAD
                     NRSLARFELKGIDPLPAGLARIEVRFLIDANGILNVTARDLHTGSQQSVEVKPSYGLT
                     DDEVEGMIAEAVDQAEADVRQRELVEARLEADRILAATEKAMSHEAWFDVGEEERQAI
                     QTALNELQLAYYSEDALLIRAQIGKVNQATTHLAELVMEAAVRAAVQGKRVDEI"
     gene            complement(5889..6452)
                     /gene="hscB"
                     /locus_tag="VFA54_13520"
     CDS             complement(5889..6452)
                     /gene="hscB"
                     /locus_tag="VFA54_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HZS51880.1"
                     /translation="MQRPSGDFFEYLALDHKLQVDLPELERQFYSLSRRLHPDIYFHR
                     SRQEREIAENAAARLNDAYRTLKDPVKRAEHLLDVLGIPRKRRDPREPRAGNTPPELV
                     EEIFEIQMLLDDVRQGDKSAASGLAHAKAKFETLLQETDASLNACFAEWDRVRRPEKL
                     REIATILDRRSYIEKALQDIVAALTDD"
     gene            complement(6569..6886)
                     /locus_tag="VFA54_13525"
     CDS             complement(6569..6886)
                     /locus_tag="VFA54_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HZS51881.1"
                     /translation="MIEVTPKAVEKIREILAKQEGGGLRLAVVGGGCSGLTYQFKLDQ
                     RPRANDNVFEYDGVKIFVDPKSYAYLEGLTLDYKESLMHSGFTFQNPNAKKTCSCGTS
                     FVA"
     gene            complement(6883..>7087)
                     /locus_tag="VFA54_13530"
     CDS             complement(6883..>7087)
                     /locus_tag="VFA54_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HZS51882.1"
                     /translation="KTLEEALVLQKTEILNELGLPPAKIHCTVLTEDAIRAAVADYKA
                     KRAARQAAAGATLEAGSKGEPSA"
BASE COUNT         1355 a         2203 c         2085 g         1444 t
ORIGIN      
        1 gatcctgggc cggcggtgga atgtcgccgg aagcttttgc tgcacgaaat tgcgccaccg
       61 tcgcaaaaga ttcagcagaa tgagtccacg tttctccgaa gtcggaagtc gtccacactt
      121 cgccgcggga ggtgagaacg atgagctgag ttccatcgga agagaaggcc acatcgaaga
      181 ccggggcgct ggccgggccc gccaaaccca gttcaatcgc gtggccgccc aggggccgcc
      241 agaaaagcgg gcgtggcgcc ggcggcaagg cctgattctg cgcccaacct gcggcgcatc
      301 ccaacaacaa gaccgtgagc gctgttctcc gcatggtact tagtaaaaat cctgcctgga
      361 gttcagctaa aaatcagttg catctctcta cgcgctgcga tagcctcagg gatcggggct
      421 ctgtttcctt tggacacagc gctatggccg cgcagcctag atcatagcgt attggggctg
      481 gaaaaatcga gggaaatctc gatttttcaa cgactcccgc tgtggatgcg aaagggatag
      541 gcaattgctg agcgacttac atgctcgtgt cggcgctgct caataaccgg aaggcctggc
      601 tggccgtgtc gattctagcc gcactcgcag ccctccctgc tccggcgcga gccgatggag
      661 atccgggcac ggcggccaag gttgtgcagg ccgaggggca ggtttccgtg ctacgcaaca
      721 gcgagctttg ggcccttttt gccggcaact ccgtacgggt cggggaaacc attgttaccg
      781 gcgcggatgg ctatgcccga ctggaagtca ccgatggcag ctcgtttgag gtatatccca
      841 actcccgggt ggtattccgc tctaaccctg gcaatttgcg ggagttactg gaagtctttc
      901 tcggcaaagt caaagtccac atccagacct tgggcggccg ccccaatcct taccgcgtag
      961 aatcgccgac ggccgtgatt tcggtgcgag gcacggtttt cgaagtcgcc gtggagtccg
     1021 ataatgttac gtgggtaggt gtggatgaag gattggtgat ggtggagcat cggctactgc
     1081 ccggcaaagc cgtgccgctg gcccctggcg aaagcctcca gatcttcccg aacgcctcgc
     1141 tggctgcggc tggaattgat aaggctcgtc ttgcgatccg gatcgccgat gccgcccgag
     1201 aagcgctcta cgtgttgcga acggttggcg tcggcggcaa gaacggcgga aagggtctgc
     1261 cgggcccggc ccccggtccc tcgccttccc cgcttccgac ggacaagcag gcgccgcctc
     1321 cgccaccgcc gcctcccccg cccccgagtg gtccctaaga tgccgcaggg ccagcccccg
     1381 gtcgaagaac ctgatattta tccatacatc gatctgaagc gggcccgctt gtgcctggac
     1441 tgcgagatga ttttcgacgc cccacagtgc ccggcctgcg ccagcgagag ctttgttccc
     1501 gtgacacgtt ggattcggcc ctcggaacgc gaaatcagcg agcgcactca gccccatcct
     1561 cccagcgcgg caaacgcagc gcctccgtcg aagccctcca agcgcctgtt gaagaaatct
     1621 ctgtatgtgg gtttgagcgc ttacggtctc tggaaaatgc tttttgagcc ggccaagcca
     1681 cggcgccgcc ggcaaccgcc gcaacctacg gaactggcgc aaaaaccagt agccggcggc
     1741 cggaccaagg ctggcagcga ctctgcatga cgcagccgtg caggagaagg cttccccttc
     1801 agcgctgctg cgtgcaagcg ggcggcggaa atgcgcggct gcaaaacagc gcggatgggt
     1861 aaatcagtag cccagtggaa aagagccaga gaggcgcggc tggcgccgcc gcacgctacc
     1921 agcgcggttc gcgcggaggc cggcccccgc cccggtggcc gcctctttcg ttcctgcccc
     1981 gccgatcgcc gaagccgcct ccgggatgga agcctgcccc ccgatccgcc ttgggacggg
     2041 cttcattcac gttgatcgag cggccctgga aatctttgcc gttcagagca gcaattgctt
     2101 tctgggcatc gtccgatgac atttccacaa acccgaagcc gcgcggcttg ccggtgtcgc
     2161 ggtcggtcat gatctgagcg gactgcactt cgccaaacgg ttcgaacagc gagctcaaat
     2221 ccgtatccgt ggcgctgaag ggaaggttgc ctacgaaaat tctcaaagtg tttctctcca
     2281 aatgggccag agcctggggc tccggcaaag gacaggtcta ttgttttgct cctcgaaaga
     2341 aggctgacca gcggcggttc agaattgagc acctcccgcg gcaaccgcgc cgtgcttcac
     2401 tgacaagagc ggaaagctga gtcgctagcg aacgcttatt ccgaagctgt ccgcaatcat
     2461 ggtagcacaa ataaaagttc caagaacgtc agcgcaagcg cggatcttgc agaattgcaa
     2521 gaataaacac ttgtccctgc aagggcgggg aggattccac tgtaccttcc gcaagccgcc
     2581 ggaaaatctt ctcgaagaac gtgcggggga tggattgatt tccagtattg acaacgatat
     2641 aggtcttgcc gtaacggtac gtgaaggaag ctccctttga cgtccggaac tccgcgcctt
     2701 cgaaggcagc gaccttttgc catatcatgt cgaaagcggg gtctcgcatg atggacgatc
     2761 ttaacaaatg gagaaccggc cgagatttgc ttcgcgcacc agttggaatc tggagctgaa
     2821 ccccctggcg caggcgctgg aagcacggcg gaaatccggc cgggagatcc tcgacctgac
     2881 cgaatcgaat cccacgcgct gcgggttcca gtacgacggc gattcgcctc ttcgagctct
     2941 cactgatcca cgggcaatgt tctatgaacc gcaccctcgc ggactgctcc ctgcgagaga
     3001 agccgtcgct cgatattatg ccgagcagcg tgctgcggtc cgcccggagc acatcgtgct
     3061 cacatcgggc tccagcgagg cgtacagctt tctttttcgc ctgctcgccg aacccggcga
     3121 taacgtgctc gttccccggc cgggctaccc gcttcttgaa tttctctctc gcctcaatga
     3181 tgttgagctg tcaagctacc gcctcatgta ccacggaaac tgggagatcg attttgattc
     3241 actcgaggcg gccgcagacg agcgcacgcg cgccgtgatc gttgtgaacc caaacaatcc
     3301 caccggatcc atcgtccggc cttccgagcg tctaatgatc gaagattttt gccggcgccg
     3361 cggcctggcg ctaatctcgg atgaggtctt tctggattac acgtttcccg aagccgccgg
     3421 ggcggccggg accttcgctg gaaccagcgg ctgtctggcg ttcgctctga atggtctttc
     3481 caaaacggcc gctctcccgc agatgaagct gggctggatc gccgcggccg gccccgccgg
     3541 cctggcggag gaagcgctgg accggcttga agtaattgca gatacctatt tgtccgtggc
     3601 cactccggcg caatgggctt taccggagct gcttgccgcg cggcgtcgca tacagcgtca
     3661 aattctggat cgtataaccg gcaatctcca ctgcctcgat caactgctga gccggcagtc
     3721 cctgtgcgcc cggtcgaaca tcgaggccgg ctggtcggcg attctccgag cgcccggaac
     3781 gttgagcgat gaagagcggg ctctcgagct gctcgataaa gacggagtgc tggtccatcc
     3841 cggtcatttc ttcgatttcg gcggcgaggg atatctggtt gtcagtctgc tgccgccgcc
     3901 tctcgtattt cgggaaggaa tctcacgaat gctcgcccgg atcaccgcgt gatgccggcg
     3961 cgctttacgg cgggcgaatc aggttgagcg tatgccgtaa aaagcgaatg gatttgcaca
     4021 agcatgcagg cagctagatt tcatccacgc gcttgccctg caccgctgcc ctgacggcgg
     4081 cctccataac cagttccgcc agatgtgtgg tcgcctggtt cactttgccg atttgggcac
     4141 ggatcagcag ggcgtcttcg gaatagtacg cgagctggag ctcgtttagc gcggtttgaa
     4201 tcgcctgccg ctcttcctcg ccgacatcga accacgcttc gtggctcatg gctttctcgg
     4261 tggcggcgag gattctgtct gcttcgaggc gcgcttccac gagttccctc tgccggacgt
     4321 cggcttcggc ctgatcgact gcttcggcga tcatgccctc cacttcgtca tcggtaaggc
     4381 cgtaggaagg cttcacttct accgactgct ggctcccggt atgcagatcg cgggccgtga
     4441 cgttcaggat gccattggcg tcgatgagaa aacgcacttc gatccgggcc aggccggccg
     4501 gcagcggatc aatccctttc agctcgaacc gcgccagact gcggttgtcg gccgccatct
     4561 cgcgctcccc ctgcaccaca tggatgacca cattggtttg cccgtcaacg ccagtggtaa
     4621 acacctcggc ggcgcttgcg ggaatcgtcg agttgcgatg aatcaacgtg gaaacgacgc
     4681 cgcccagcgt ctcgatcccc agcgacaacg gcgtgacgtc gagcaacagc atgtcccgct
     4741 gaacaccggc caaaatgcct gcctgcacgg cggcgcccag ggcgacgacc tcttcggggt
     4801 tcagctcgat gtgcggcttt cgctggaaca tgagctccac cgcctggcga acaagcggaa
     4861 tgcgggtgga gcctccgacg agcacaacct cgtcgatctg ccggatgtga acgtgcgcgt
     4921 cttcgatcgc ctgccggcag ggatcgatgg tcttttcgac aatgccggcg atccattttt
     4981 cgaaaagcgg ccggtcaatc caacggcggt aatggcggcc ttgccaggaa aatcgaattt
     5041 ccgcctgctt ctcggtggat aaagcatgtt tcgcttccat cacggcccgg cgcagtaact
     5101 ggacagcgat cgcgtctccg gagaggtctt ccgcccattc ggaagccaaa tcttcgagag
     5161 cgatttgcac gagcagattg tcaatgtcat caccgcccaa atgggtatcg ccgttggtgg
     5221 cgagcacctc gaagatgccg ccctgcagtt tgaggatcga aatgtcaaag gttccgcccc
     5281 ccaggtcgta gaccgcgata atgcccttct tccgctggtc gagcccgtag gccagagccg
     5341 cagcagtcgg ttcattcacc agccggagta cgtccaggcc ggcgatcctg ccggcgtctt
     5401 tggtcgcctg tcgctgggcg tcgttgaaat acgccgggac tgtgatgacc gctttggtga
     5461 ccggaacgcc caggcaagcc tccgcattgc gccggagccg gcgtaagacg tgcgcggaaa
     5521 tttccggcgg agtcaaagtc ctgtctccca aggcgatccg gattaccgac tcgctcccct
     5581 ccgttatgcg gaagggaaac cagtgcagtt ccccggcgac atcggcgatt cctttcccca
     5641 tgagccgctt tacggaataa accgcgcgat cagggcggga aagcaggacc tcagcggccg
     5701 ggcggccggc tgccagttct ccgctgccgg tcatagccac cacgctcggg acgattcgtt
     5761 ctccacctgg tccagcgata atcttgggcc ggctggtttc catgcaagcg atcagactat
     5821 tggtggtccc caggtcgatc cctacgatca cctccgggct ttgagtgttc gagtcgcgtt
     5881 cgcggagatc agtcatcagt cagagccgcc acgatatcct ggagcgcttt ttctatgtag
     5941 ctgcggcgat cgagaatcgt agcaatctcc cgaagttttt ccggccggcg gacgcggtcc
     6001 cattcggcga agcaggcatt cagtgaagca tccgtctcct gaagcaaggt ttcaaatttt
     6061 gccttcgcat gcgccaagcc actggccgcc gatttatcgc cctgccggac atcatccagc
     6121 agcatttgga tttcgaatat ctcctccacc aactccggcg gcgtgttgcc ggcgcgtggc
     6181 tcccgaggat cgcgccgctt tcgcgggatc cccaacacat cgagcaagtg ctcagcccgc
     6241 ttcacggggt ccttgagagt ccggtaagcg tcgttcaacc gggcggccgc attctctgcg
     6301 atttcccgct cttgccggga gcgatggaag taaatatcgg ggtgcagccg gcggctcaga
     6361 ctataaaatt gccgttcgag ctccggcagg tctacctgga gcttatggtc caaagccaga
     6421 tactcaaaaa aatcacccga aggtctctgc acactcccgc aatgatcgca aaagtgcaaa
     6481 cccgccgctc gcccgcaatt ccagcaggca accgattttt tctcttcgga gggaagggga
     6541 aaggccggca ttctgtacgc gccgccgctt aagccacaaa cgaggtcccg caactgcacg
     6601 tcttcttggc gttgggattc tgaaaggtga accccgagtg catcaaggat tccttgtaat
     6661 cgagcgtcag cccttccaga tatgcgtaac tcttgggatc gacaaagatt ttgaccccgt
     6721 cgtattcgaa aacgttgtcg ttcgccctcg gccgctggtc cagcttgaat tgataggtca
     6781 agccgctgca cccgccgccg accaccgcca gacgcaagcc accgccctcc tgcttggcca
     6841 ggatctctcg aattttttcg accgctttgg gggtgacctc aatcatgccg atggctctcc
     6901 tttgctgccg gcttcgagtg tcgcgcctgc tgcggcctgt ctcgcggccc tcttggcttt
     6961 gtaatcggcc acggccgccc ggatagcgtc ctccgtcagc accgtgcagt ggatcttggc
     7021 gggaggcagg cccagttcat tcaaaatctc tgttttttga agcaccaaag cttcctccag
     7081 ggtcttg
//
LOCUS       DASREK010000250        13634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2267500, whole genome shotgun sequence.
ACCESSION   DASREK010000250 DASREK010000000
VERSION     DASREK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13634
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2267500"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFA54_13535"
     CDS             complement(<1..602)
                     /locus_tag="VFA54_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HZS51883.1"
                     /translation="MQLTALDWAVVAGYFILNLLIGLYYKGRAGRSLEEFFVSGRDVP
                     WWLAGTSMVATTFAADTPLAVTGMVAKGGIAGNWLWWNFVFSGMLTVFFFARLWRRCG
                     VLTDVEFAEIRYAGRPAAFLRGFRALYLGVPINCIVLGWVNLAMVKILMLILGITKVQ
                     AIAAVLGLIALTCSISTLAGLWGVLVTDAVQFAIKMGMVIV"
     gene            complement(631..3279)
                     /locus_tag="VFA54_13540"
     CDS             complement(631..3279)
                     /locus_tag="VFA54_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51884.1"
                     /translation="MRKRIITALILALLLAPAGGAYVLLTNGVPYTFLLPQPETASTF
                     YNGDLGYSIYVPPGSTKLTIDFAPVSLFYDVDLLVRYNAEVGMSGTGPLLYDYRTSGG
                     PGNKEIVVTPQSSPPLQSGTYYIAFLVNSTDIRVEASITATVEGGSIPPVRVVAMSTF
                     DKDLDGWTANTSTGGLPGASQGDPASTLVWDSTGGNTGGFAHYYQAESLAAGAFVAPA
                     KFLGNLSGLEDPRFEFDFVQLTGSETVYSVEIRIFGANTAYSWQGPIPPSPVSNVNPC
                     VRLVLNPVTNKYDCEGESNIWSHYTAALKQEFWNRLTGDASLATVLSNVQRIEVLANL
                     TFHGGSSGIDNFALLSRGTGPIQPVAAGNTSFASGVDGWTRNYPASNLAGATAGNADS
                     TFRWVAFDGNPAGYIRLNSSGGANPDALVAPDRFLGDYTGLPSPQFEFDYLHLSSSGA
                     SKPVQIRLVAADSVFVWTGPVPPTGLWTHYVAPLKDSIWQRVSGATAFVQALANVERI
                     EISAQQSGGPESDGLDNFWLMPSPITPAPPALSASPSALSLEAVARSSNPSTSLGITS
                     IGGGQPLNFTVAASGGSWLRVSAANGTTPLLLKIWADITGLAPGAYTGQVTVTAPGTS
                     LDPRTVAVNLTVNQQAGMIPRIYPGGIVNSASYQPLLAAGALGTIFGTGLSAGPSLTA
                     SFLPGTSSLPTRLQGTRVLVNDTSGALIAEAPLFYVSDSQINFQLPFEAFGRSQVLVA
                     VDNNGSLSDPQTVSLAPASLGIFTYDANRAVAVNEDETVNSPENPAAHGSALTVYMTG
                     QGVVTPTVPTGAAALDRPLSRAPFPAQATIGGAPAKLLFVGLAPGMVGVLQVNLVVPS
                     AAPSGDSPLVFTMNGSTSNSAIVTVR"
     gene            3468..4487
                     /locus_tag="VFA54_13545"
     CDS             3468..4487
                     /locus_tag="VFA54_13545"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HZS51885.1"
                     /translation="MIIGLTGTNGAGKTTVCEHLKRLGFAYYSLSDEIRAELARRGQP
                     ATRENLIEMGNRLRREGGPAILAERVKAKLEPGCNYVVDSVRNPAEVEALRRLPDFHL
                     LHVDAPIALRYERARARRDARVPATFEEFVEQEEREMHSADPASQQLRACYELADERL
                     VNAGTKEELLARLDELVRRWAMSARRPDWDEYFMSIARIVASRSNCMKRKVAALIVKD
                     RRIISTGYNGTPRGVKNCNEGGCPRCNSLAPSGTNLEECFCSHGEENAIVQAAYHGIS
                     LKDSTLYSTFSPCLICTKMIINAGIREVVYNMDYPLNENALRLLAEAGVAVHQAGSGR
                     SSAAG"
     gene            complement(4594..6039)
                     /locus_tag="VFA54_13550"
     CDS             complement(4594..6039)
                     /locus_tag="VFA54_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZS51886.1"
                     /translation="MENVANEIFKGLSRRHFLRSSAGAALAALMSAEPRPLWPQQKPR
                     IPPRADCFILLWMAGGMAQTEMFDPKAYTPFEPGLPSSRVLSTFPAIDTVVDNIKISQ
                     GLDRIGSVMDRATLIRTHRVGDLGHILHARHQYHWHTGYPPPQSVAAPHLGAVIARTL
                     GPKNPDVPAFIDIGQRLEMGGESDGLKAFHTAGFLGTEYGPFLIPEPEDAAATVRPAH
                     LTQARFRERYERYRRLVASSPILRYGSGYQQESLLRSVENAHRLLNSPAAKAFDLSLE
                     PKRSYDVYNTGRFGLGCLLARRLAEAGARFIEVTTEYVPFRHWDTHENGHERMIGLKK
                     MIDAPVAQLVLDLEERGLLDRTLVLVASEFGRDMMVEGKPDKRVQEQASIHQPDIMTE
                     PKHYGMHRHFTEACSVLAFGGGFKKGFLYGKTADERPCKIVEKPVTIEDLHATIYHAL
                     GIPPDLAYEVEQRPFHVTKDGQGKPVMDLFS"
     gene            complement(6066..8414)
                     /locus_tag="VFA54_13555"
     CDS             complement(6066..8414)
                     /locus_tag="VFA54_13555"
                     /inference="COORDINATES: protein motif:HMM:NF019219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 domain-containing protein"
                     /protein_id="HZS51887.1"
                     /translation="MWRLLAFLIVLAMPGAARAQAAGQSRKVDFVHNVQPILAAKCGA
                     CHGGEKAEGGLRLHTRAEILKGGVSGPAIVPGNSRDSLLIQRVSGFRAPVMPATGDHL
                     NEKEMQTLRDWVDQGAPLSNAAPETKATSGIPPRRPAVPKGSLHGSANPIDHFVAAYL
                     ARHSYPPSAPVPDAVFARRAYFDVWGLPPTPEQLDMFLGDTRPGKRTRLIRELLGNNE
                     NYSEHWISFWNDLLRNDEGVNYAGMRQSITGWLLRSLRENMPYDQFARALLNPVRPDD
                     PAGFLLGVNWRGDVSASQTPVMQAAQNTAQVFLGVNLKCNSCHDSFISDWKLKDAYGL
                     ASFFSPRGEMDVYRCDVKTSEKSTPKFLFPELGGVEATASLEERRAAAARLFTSLENG
                     RFPRTLVNRIWQRLFGRGLIEPVDDMAAQAWDSDLLDWLASDFADHHYDLKFLLETIL
                     TSQAYQLPAVHLPNREDKNYAFRGPVERRLTAEQFADAVSSITGEWRVLAPVKAGAGT
                     YVREWRLKSSPLTRALGRPIRDQVFTERNSEPTTLQALELVNGETMTGLIHRGAKRIL
                     GELPPAPPNLFDSGEMRSGRAVVDVDISGAQQLRLLIEDIDSYDPSRVIAGWADAELV
                     SPGGVTRLTALETRSKFTRGPLRIGTQTYSDAVTAATPSELVYDIAGRGFTRFRATVG
                     LDEKCRTDEINPRVRFFVFTEAPDRRQLVRVAPGAPVAAPPIERDPEALVTRIYRHAL
                     GREPNAGERKTALEFLSPKLTSEGLEDLLWSVFLSPEFQFIN"
     gene            8535..9047
                     /locus_tag="VFA54_13560"
     CDS             8535..9047
                     /locus_tag="VFA54_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51888.1"
                     /translation="MSRLSRCVWILVFLLAAASAQDPAQNVPKLSGFTYWPAAKLKSY
                     PSVLKAKEPTVRGATKTMMAAERLGDHGGYSFMEERRDVDGVPEIHAHLDDIFIVQDG
                     EATLLYGGRIEGGEELRPGETRGGRIVGGTMQKVAAGDMAVIPAGMPHQTIVSKGKSY
                     TFMVIKIEKK"
     gene            complement(9192..10343)
                     /locus_tag="VFA54_13565"
     CDS             complement(9192..10343)
                     /locus_tag="VFA54_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS51889.1"
                     /translation="MWERVRNIVRKEFIQVLRDPRMRGVLFIPPLIQLLIYGYTVNLD
                     IENSRIAWMDQDRTPQSRELLAGFQGSPRFAIVAAPSSEEQVQTLLDRGHVQAVIRVL
                     PGFGRDIERGRPVSVQVLVDGANSNTASLVSSYASSVIANYSSKIMLAQQNMRIMARS
                     PAAAPNFSAPSIASRTRVWFNPDLESRNYFVPGVVVNIIMIVTLSLTAQAIVREKEIG
                     TMEQLMVTPIRPIELIIGKTLPFAIIGLFDVVLVISAALLIFRTPLRGSPVLLLFCSI
                     LFLLTTLGAGLFLSTISRTQQQAVMSSFFFTAPAFMLSGFAFPIRNMPLPVQYLTYLN
                     PLRYFIEIVRGIFLKGVGMAVLWPQMAALLIYGVAILGLSAVRFRKRLD"
     gene            complement(10348..11481)
                     /locus_tag="VFA54_13570"
     CDS             complement(10348..11481)
                     /locus_tag="VFA54_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS51890.1"
                     /translation="MSRRRLRAIFQKELRHIVRDVRSLAIALAMPLLFLVLFGYALSL
                     DVDRIPTLVYDSDQSAESRELIQRFAGSRFFQIAGLVDNYRAMEQGIDSGRILMGLAI
                     PRGYSRRIGSGAEAGVQVLLDGSDSNTASIALGYADSVLRNYSLQLRSEGQNRKGGVT
                     LSAPVEARLRVWYNSSLQSKNYIVPGLIAVILMIITSMLTSLTIAREWEMGTMEQLLS
                     TPVRPAEMVLGKMLAYFVVGVVDALVAVLVGVFLFGVPLRGSILLLAATGCLFLCGVL
                     FWGIYISAAAKSQMMAFQLGMLSSFLPAMLLSGFIYAIENMPLPIRLITYLTPTRYFV
                     TILKGIFLKGIGLRTLWAEIVFLAVFGIAVFVGATRKLRAKVA"
     gene            complement(11478..12419)
                     /locus_tag="VFA54_13575"
     CDS             complement(11478..12419)
                     /locus_tag="VFA54_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51891.1"
                     /translation="MNGWTVEIHDLVKTFGPFVAVDRVSLEVRAGEIFGFLGPNGAGK
                     STTIRMLCGLLTPTSGSARVAGFDVATAPEQVRRNIGYMSQKFSLYDDLTVEQNIDFF
                     AGMYGVPKDRRRERKDYVLQMAQLAEQRKTLTAALSGGWKQRLALGCAILHDPPIVFL
                     DEPTSGVDPIARAAFWDLIRDLSGTGHTIFVSTHYMDEAEYCHRIALMYAGRVIALGE
                     PGTLKRELESHRLLQLDSSDPLESMRAIETQPGILDVAVFGAGLHVTVDDVEASSARI
                     RQALAARQIEIRRLEPIEPSMEDLFVALIEAEERKSS"
     gene            complement(12416..13159)
                     /locus_tag="VFA54_13580"
     CDS             complement(12416..13159)
                     /locus_tag="VFA54_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51892.1"
                     /translation="MEALIETRGLTRRFGAVTAVDHLDLTVNAGEIFGLVGPDGAGKT
                     TTLRLLCGLIDPTEGSAHVGGRDSAREPQAVKDQIGYMAQRFGLYGDLTVQENMDFYA
                     DLFGIVGSERAELAARLLRMTRMEPFRERQASRLSGGMKQKLALMCTLLHRPRILFLD
                     EPTNGVDPVSRRDFWTILYQLLKDGITIVMTTAYLDEAERCNRVGLMHRGRLVRCDTP
                     EALKHSAGAANLEGVFIETIRQAEALETR"
     gene            complement(13162..>13634)
                     /locus_tag="VFA54_13585"
     CDS             complement(13162..>13634)
                     /locus_tag="VFA54_13585"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS51893.1"
                     /translation="EMKRREQELATRRAEVARAKANLALIDSQLADTTAVSPVDGVVL
                     VKSADVGEVLAAGATVVTIGDIDHPWLRGYINETDLGRVKLGSKARITTDSYPGKAYN
                     GRISFIASEAEFTPKQIQTKQERVKLVYRIKIEVDNPQHELKSNMPADAEILLD"
BASE COUNT         2720 a         4200 c         4193 g         2521 t
ORIGIN      
        1 acgatcacca tccccatttt gatcgcgaat tgcacggcgt cggtaaccag gacgccccac
       61 aatccggcga gcgtcgagat cgagcaggtg agcgcaatca gcccgagcac ggccgcgatg
      121 gcttggacct tggtgatacc cagaatgagc attaggattt tgaccatggc caggttgacc
      181 catccgagga cgatgcagtt gatcggcacg cccaggtaca gcgcccggaa cccccgcagg
      241 aaagcggcgg ggcgccccgc gtagcgaatt tccgcgaact cgacgtcggt cagcacgccg
      301 cagcggcgcc aaaggcgcgc gaaaaaaaac accgtcagca ttccgctgaa gacgaaattc
      361 caccatagcc agttgccggc gatgccccct ttggcgacca tgcctgtaac ggccagcggc
      421 gtgtcggcgg cgaacgttgt agctaccatg gaagtcccgg cgagccacca ggggacatcg
      481 cggccggaga cgaaaaattc ttccagactc cggcccgcgc ggcccttata gtagagccct
      541 atgagaaggt tgagaataaa gtagccggcg acgacagccc aatcgagcgc agtcagttgc
      601 atgagggtgc tccgaagagc actccctatg ctacctcaca gtgactatgg cgctgttgct
      661 ggttgagccg ttcatggtga ataccagggg agagtcgccg ctgggggcgg cgctcggaac
      721 aacgagattc acttgcagaa ctcccaccat gcccggagcg aggccgacga acaaaagttt
      781 cgccggcgcc ccgccaatcg tggcctgggc cgggaacggg gcgcgggaca aaggacggtc
      841 cagggctgcg gcgccagtgg gaaccgtggg agtgactacc ccctgtccgg tcatgtagac
      901 ggtcagtgcg ctgccgtggg cggctggatt ctccggagag ttgacggttt catcctcatt
      961 taccgccacc gcgcggttgg catcgtaagt gaaaattccg agcgaggctg gcgcgagcga
     1021 aaccgtttgc gggtcgctca gtgacccgtt gttgtctacc gccactaaaa cctgagagcg
     1081 gccgaaagct tcaaaaggaa gctggaaatt gatttgggag tcgctgacgt aaaacagcgg
     1141 agcttccgcg atcaacgcgc cggaagtgtc attgacgagc acccgcgtgc cctgcaatcg
     1201 cgtgggcagc gaggaagtgc cggggaggaa cgaagccgtt aacgatggtc ccgctgacag
     1261 gcccgtcccg aagatcgttc ccaatgcgcc ggctgccagc agtggctgat aacttgccga
     1321 gtttacgatg cctccaggat agatccgggg gatcatgccc gcctgctggt tgaccgtcag
     1381 attcacggcg accgtgcgag gatcgagcga tgtgcctgga gccgtcacgg tcacctggcc
     1441 tgtgtaagcg ccgggagcca ggccggtgat gtccgcccag atcttcagca acaggggcgt
     1501 ggttccgttg gcggcggaaa cacggagcca gcttccgccg gaggccgcta ctgtaaaatt
     1561 cagcggctgg ccgccgccga tcgacgtaat ccccagcgag gtcgagggat tcgaactccg
     1621 ggccaccgcc tccagcgaga gcgccgaagg gctggcgctg agcgccggcg gggcgggcgt
     1681 gatcgggctg ggcatcaacc agaagttatc caggccgtcg gactccgggc cgcccgattg
     1741 ctgggcgctg atctcgatcc gttccacatt ggccagcgcc tgaacaaagg ccgtcgcgcc
     1801 ggagacgcgc tgccagatag agtctttcag cggggcgacg taatgggtcc aaagtcctgt
     1861 gggaggcaca ggccccgtcc acacgaatac cgagtcggcg gcaactaaac gaatctgaac
     1921 gggcttgctc gccccggagc tggacaagtg caaataatca aactcgaact gcgggctggg
     1981 caggccggta taatctccga ggaaacggtc gggcgctacc agagcgtccg gattggctcc
     2041 gccggagctg ttcaggcgga tatatccggc cggattcccg tcaaaggcca cccatcggaa
     2101 tgtcgaatcg gcattcccgg cagtggcgcc ggccaaattc gaagccggat aattgcgagt
     2161 ccaaccatcc acgcccgagg caaagctggt gttgccggcg gcgacaggtt gtatggggcc
     2221 ggtcccgcgc gataagagcg caaagttatc gattccgctg gatccgccgt gaaacgtcag
     2281 attcgccagc acctcgatgc gctgaacgtt cgatagcacg gttgccaggc tggcatcgcc
     2341 ggtgagccgg ttccaaaact cctgcttcag agcagccgtg tagtgggacc agatattcga
     2401 ttcgccctcg cagtcgtatt tatttgtaac gggattcagc accagccgca cgcacggatt
     2461 tacgttggac acaggcgatg gcggaattgg cccttgccag ctatatgcgg tgttggcgcc
     2521 gaagatccgg atttccaccg aatagaccgt ctcggacccc gttaactgga cgaaatcaaa
     2581 ctcgaagcgc gggtcctcca gacccgatag attgccgaga aatttcgctg gggccacgaa
     2641 cgcgcctgcg gccaggctct cggcctgata gtagtgggca aagccgcccg tattgccccc
     2701 ggtcgaatcc cataccagcg tcgaggcagg gtcgccctgg cttgctccgg gcaggccgcc
     2761 ggtgctggtg ttggcggtcc agccgtcgag atccttatcg aatgtagaca ttgccactac
     2821 gcgcacgggc ggtatggagc cgccttcgac ggtagccgtg atactggcct cgacccggat
     2881 gtcggtcgag ttcaccagga aggcaatgta gtaagtgccg ctttgcagcg gcggggagct
     2941 ttggggagtg acaacgatct ctttgttgcc ggggccgccg ctggttcgat aatcgtaaag
     3001 cagcgggccg gttcctgaca ttccgacctc cgcgttgtag cgcacaagca ggtcgacgtc
     3061 atagaaaagg gagaccgggg cgaagtcgat cgtcagcttg gtggatcccg gggggacgta
     3121 tatcgagtat ccaaggtctc cgttataaaa tgtgctggcg gtctctggct ggggcagcaa
     3181 aaatgtatac ggtacgccgt tcgtcaacaa aacataggct ccgccggccg gcgccagcag
     3241 cagcgccaaa atcaaagcgg tgataattcg ctttctcaag aagcccctcc accaaccttc
     3301 atcggcaaaa agatctggct cagcgcatat tactccgcta ccgatggtgc agtattgagg
     3361 tacttgccgg aaatccggcg ttcacactag ataaaccgga gttttgacct aactggaggc
     3421 gcacagcgtt ttggctggcc cgctcggggg ctgttaccat ggggcgaatg attattggac
     3481 tgaccggcac caatggagcg ggcaaaacaa ccgtctgcga acacctgaaa aggcttgggt
     3541 ttgcatatta ctccctttcc gacgagatcc gcgcggaact ggcgcgccgc ggccagccgg
     3601 ccacacggga aaacctgatc gagatgggca atcgcctgcg ccgcgagggt gggccggcca
     3661 ttctcgccga gcgtgtgaaa gcgaagctcg agccgggctg caattacgta gtcgattcgg
     3721 ttcgcaaccc cgccgaggtc gaggctttgc ggcggctgcc cgattttcac ctgctgcacg
     3781 tggatgctcc gatcgccctg cggtacgaac gcgcccgcgc ccgccgcgat gcgcgcgtcc
     3841 cggccacatt cgaggaattc gtggagcagg aagagcggga aatgcacagc gccgatcccg
     3901 cctcccagca gttgcgcgcc tgttacgaat tggcggacga gcgcctggtc aatgcgggga
     3961 ccaaagagga gctgctcgcc cgcctggatg agctggttcg gcgttgggcc atgtcggcca
     4021 ggcgccccga ttgggatgag tacttcatga gtattgcccg catcgtcgcc tcgcggtcca
     4081 actgcatgaa gcggaaagtc gccgcgctga ttgtaaaaga ccgccgtatc atttccaccg
     4141 gctacaacgg cacgccaagg ggagtgaaga actgcaacga aggaggctgc ccgcgctgca
     4201 actctctggc gcccagcggt acaaatctgg aagagtgctt ttgctcgcac ggcgaggaaa
     4261 acgccattgt gcaggcagcg taccacggca tcagtttgaa ggactccact ctgtactcga
     4321 ctttctcccc ctgcctgata tgcacgaaga tgatcatcaa cgctggcatc cgtgaagtgg
     4381 tctataacat ggattatccg ttgaacgaga atgctctccg gctgctggct gaggccggag
     4441 tcgccgttca ccaggcgggc agcggcagga gcagcgcggc gggctgaact ttccggaaac
     4501 tgtcgctttc tcggcggctc ccttcaccgg ctggcgtatc gaaggcggtt ctcaggtcag
     4561 cgacaaccgt cgtctccagc gcggttaatg gcttcaggag aatagatcca tcacgggctt
     4621 gccttggccg tctttcgtga catgaaatgg gcgctgctcg acctcgtatg ccagatcggg
     4681 cggaatgccc agggcgtggt agattgtcgc gtgcaggtct tcgatcgtca ccggcttttc
     4741 cacgattttg cagggccgct cgtcggcggt ctttccgtag agaaaacctt ttttgaagcc
     4801 gccgccgaat gccagcacgc tgcacgcttc ggtgaagtgc cggtgcatgc cgtaatgctt
     4861 cggctcggtc atgatgtccg gctgatgaat ggaagcctgc tcttggacgc gcttatccgg
     4921 cttgccctcc accatcatgt cccgcccgaa ctcgctggcc accaagacga gtgttcgatc
     4981 gagcagcccg cgctcttcca aatcgagcac aagctgcgcc accggcgcgt caatcatttt
     5041 cttcaggccg atcatgcgct catggccgtt ctcatgggtg tcccaatggc ggaaggggac
     5101 gtattcggtg gtcacctcga tgaagcgcgc gccggcttcc gccagccgcc gggccagaag
     5161 acaacccagg ccgaagcgtc ccgtgttgta gacgtcatag cttcgcttcg gctcgagcga
     5221 tagatcaaaa gccttggccg ccggcgagtt cagaagccgg tgcgcatttt ccactgagcg
     5281 cagcagagat tcctgctggt agccgcttcc gtaccggaga atcgggctgc tggccaccag
     5341 acggcggtac ctctcgtaac gctcgcgaaa ccttgcctgt gtcaaatgag cgggacgaac
     5401 cgtcgccgcc gcatcttccg gttccggaat caaaaagggt ccgtattcag tcccgagaaa
     5461 accggctgtg tgaaacgcct tgaggccgtc gctttcgccg cccatttcca gacgctgccc
     5521 gatgtcaatg aacgctggaa cgtccgggtt ctttgggccc agagtccggg caatcacagc
     5581 gcccaggtgg ggcgcagcga cggactgtgg cggggggtat ccggtatgcc agtggtattg
     5641 gtgccgcgcg tgcagaatgt ggcccaggtc gcccacgcgg tgcgtgcgga tcagcgtagc
     5701 gcggtccatt actgaaccaa ttcgatccag accctgcgag atcttgatgt tgtctacaac
     5761 cgtatcaatc gccggaaacg tgctcagcac acgactcgac ggcaagccgg gttcgaaagg
     5821 cgtgtacgcc ttcggatcaa acatttccgt ctgcgccatg ccgcccgcca tccagagcag
     5881 aatgaaacaa tccgctcgtg gcggaatcct cggcttctgt tgcggccaca acggccgcgg
     5941 ctcggcgctc atcagcgcag cgagtgcggc gccggccgag gagcgcagaa aatgccgccg
     6001 gctcagccct ttgaatatct cgttggccac gttctccatc gtcgcaacct cgaattagca
     6061 gcccatcaat tgatgaattg aaactccgga gagagaaaaa cgctccagag cagatcctcc
     6121 aaaccttcgg aggtaagttt tggcgaaaga aattctaaag ccgtttttcg ctctcccgcg
     6181 ttcggctcgc ggcccagcgc atgacgataa atgcgcgtca ccagtgcttc gggatcccgc
     6241 tcaatcgggg gagccgccac aggcgctcca ggggctacgc gcacgagttg ccgccggtcc
     6301 ggtgcttcgg tgaacacgaa aaatcggacg cgcggattga tttcgtccgt gcggcatttc
     6361 tcatcgagac ccacggtggc ccggaaacgc gtaaagcccc tgccggcgat gtcgtacacc
     6421 agttccgagg gcgttgctgc tgtgacggcg tccgagtaag tctgggtccc aattcgaagc
     6481 ggccctcggg tgaacttgga cctcgtttcg agagcggtca aacgcgtcac gccgcccggc
     6541 gagaccagct ccgcatcggc ccagccagcg atgacccggc ttgggtcgta ggaatcgatg
     6601 tcttcaatca gcagccgaag ctgctgcgcg ccggaaatat cgacatccac aaccgcccgc
     6661 cccgagcgca tctcgccgct gtcgaacaga ttggggggcg ccggcggcag ctcgcccaaa
     6721 attctctttg cgccccggtg gatgaggccg gtcatggtct cgccgttgac gagttccagc
     6781 gcctggagcg tagtcggctc gctgttacgc tccgtgaaaa cctgatcgcg gatcggccgg
     6841 ccgagagcgc gcgtaagcgg gctcgatttc aagcgccatt cccgcacata ggttccggct
     6901 ccggctttga cgggcgccag cacacgccac tctccggtga tggaagagac ggcatcggcg
     6961 aattgctcgg cggtgagccg gcgctccacg ggcccacgga aggcatagtt cttatcttct
     7021 ctgttcggta gatgcacggc cggcagttgg tacgcctggg aggtgagaat tgtttccagc
     7081 agaaacttca aatcgtagtg gtgatcggcg aaatccgaag ccagccagtc gagcaggtcg
     7141 gagtcccatg cctgcgcagc catatcgtct actggttcga tcaggccgcg gccgaaaagc
     7201 ctctgccaga tgcggttgac caaggtgcga ggaaagcggc cgttttccag cgaagtgaac
     7261 agccgcgccg cggccgcgcg gcgttcctcg agggatgctg tggcctccac gccgccaagc
     7321 tccgggaaga gaaacttcgg cgtggatttc tcgctggttt tcacatcaca acgataaaca
     7381 tccatttccc ctcgaggtga gaaaaagctg gcgaggccgt aggcgtcctt cagcttccag
     7441 tcgctgatga agctgtcgtg gcaggagttg catttcaagt tcacgcccag aaagacctgc
     7501 gcggtgttct gggccgcctg catcaccggc gtctggctgg cgctgacgtc gccgcgccag
     7561 tttacgccca acagaaagcc ggccgggtca tccggccgga ccggattcaa caaggcgcgg
     7621 gcgaactgat catagggcat gttctcccga agggatctga ggagccagcc ggtgatcgac
     7681 tggcgcatgc ctgcgtaatt cacgccttca tcgttccgca gcaggtcgtt ccaaaagctg
     7741 atccagtgct cgctatagtt ctcgttgttc ccaagcagct cgcgaatcag ccgcgtgcgc
     7801 ttacccggcc gcgtatcgcc gaggaacatg tctagctgct ccggcgtggg cggcaggccc
     7861 cacacgtcga agtaggcgcg ccgcgcaaaa acggcgtctg gcacaggcgc ggaaggcgga
     7921 tacgaatggc gcgccaaata agcggccaca aagtggtcta ttggattagc tgagccgtgc
     7981 agagagcctt tcggcactgc gggccggcgc ggcggaatgc ctgacgtcgc tttggtttcc
     8041 ggcgcggcat tgctgagcgg cgctccctga tccacccagt cccggagcgt ctgcatctct
     8101 ttttcattga ggtgatcccc ggtggccggc ataaccggcg cgcggaagcc ggaaacgcgc
     8161 tggatcagca ggctgtccct gctatttccg ggcacgatcg ccgggccgct cacgcctcct
     8221 ttcaggattt cggcccgcgt gtgcaggcgc agcccgcctt ccgccttctc gccgccgtgg
     8281 cacgctccgc atttcgcagc caaaatgggc tgaacgttat ggacgaaatc gactttccgg
     8341 gattgcccgg ctgcttgcgc gcgggccgcg cccggcatgg ctaagacgat cagaaacgcc
     8401 aggagccgcc acataggcct tgcaggaatt atcgcacagg cctcttagca gggatatctg
     8461 aaaacgctgt cctatactcc gctcggcttg aggcttccgc cgcctcgagg gacccacaat
     8521 ttcaaggagg gggaatgagc cgcctgtctc gatgtgtctg gattctcgtc tttcttctgg
     8581 ccgccgcctc ggcgcaggac cctgcgcaga atgttcccaa gctttccggc tttacctatt
     8641 ggcctgccgc gaagctcaag agctatccat cggttctcaa agccaaggag cccaccgtgc
     8701 ggggcgccac gaaaacgatg atggcagccg agcgccttgg cgatcacggc gggtatagtt
     8761 tcatggaaga gcgccgtgat gtggacggcg tgccggagat ccacgcgcat ctcgatgaca
     8821 tcttcatcgt gcaggatggc gaagccaccc ttctctacgg cggcagaatc gaaggcgggg
     8881 aggagctgcg tcccggagag acgcgcggcg gcaggatcgt gggcggcaca atgcagaaag
     8941 tagcggccgg agacatggcc gtgatacccg ccggcatgcc gcaccagacc atcgtgtcca
     9001 aggggaagtc ctacaccttc atggtcatca aaattgaaaa gaaataagcg cccgggctgc
     9061 cggggaaggc gcagcccaat tatctctcct gggcgtccgc gaattggcgg ccgccggcgc
     9121 ggccgcattc cggtttggcg ctgctctgcc gggccgcctc tcgctttgga gggcggggac
     9181 ggccgaagca actaatccag tctctttcgg aaacgcacgg cgctcagccc caggatagcg
     9241 actccgtaaa tgagcagcgc ggccatctgc ggccatagga cggccattcc cacgcccttg
     9301 agaaaaattc cgcgcacaat ctctataaag tatcgcaggg ggttcaaata ggttaggtac
     9361 tgcaccggga gcggcatatt gcgaatgggg aatgcaaagc cgctcagcat gaaggccggc
     9421 gcagtaaaga aaaacgacga cattacggcc tgctgctgcg tgcgcgagat tgtcgaaagg
     9481 aacaggccag cccccaaggt cgtcagcaaa aacagaatcg agcagaaaag cagcagcacc
     9541 gggctgcccc gcagcggcgt ccggaaaatc agcaacgccg cggagatgac cagcaccaca
     9601 tcaaacaagc cgatgatggc gaagggcaga gttttgccga ttatcagctc gatcggccgg
     9661 atcggcgtca ccatgagctg ttccatggtt ccaatttcct tttcccgcac gatcgcctgg
     9721 gcggtcaacg acaacgtgac gatcatgata atgttcacca ccacgcccgg tacaaagtag
     9781 ttccgactct cgaggtcggg attgaaccag acccgcgtcc ggctggctat gctgggcgcg
     9841 ctgaaattgg gagcagccgc cggcgagcgc gccataatcc gcatattctg ctgcgcgagc
     9901 atgatcttgc tggagtaatt cgcgatgacg ctggaggcgt agctggagac cagcgaggca
     9961 gtgttggagt tcgcgccgtc caccagaacc tgcaccgaaa cggggcgccc gcgctcgata
    10021 tccctcccaa atccgggcag cacacgaatc accgcctgca cgtggccgcg gtccagcagc
    10081 gtctgcacct gctcttcact tgacggcgca gccacgatcg cgaagcgggg cgatccctga
    10141 aagccggcga ggagctcgcg gctctgcggg gtgcggtcct ggtccatcca ggcgatccgg
    10201 ctgttttcga tgtccaaatt cacggtgtat ccgtaaatta acagttggat gagcggagga
    10261 atgaacagta caccgcgcat acgcggatcg cggagcacct ggatgaattc cttgcggaca
    10321 atattgcgga cccgctccca cataaattca ggccactttg gcgcgcagct tgcgcgtcgc
    10381 gccgacaaac acggcgattc cgaaaaccgc caggaaaaca atttcggccc acagagtccg
    10441 caacccgatg cctttcagaa aaattccctt cagaatcgtc acgaaatagc gggttggcgt
    10501 tagataagtg atcaaccgga tgggcagcgg catgttctcg atggcataaa tgaagccgga
    10561 aagcagcatc gcgggcaaaa acgaactcaa catgcccaac tggaacgcca tcatctgcga
    10621 cttggccgcc gctgaaatat aaatgcccca gaacaggact ccgcaaagaa acaggcagcc
    10681 ggttgccgcc agaagcagaa tgcttccgcg caaaggcacg ccgaagagga acacgcccac
    10741 cagaaccgcc accagtgcgt ccactactcc gactacgaaa tacgccagca tcttgccgag
    10801 aaccatttcc gccggccgca cgggagtcga gagcagttgt tccatggtcc ccatctccca
    10861 ttcgcgtgcg atggtaagcg aggtgagcat ggaggtgatg atcatcagga tcaccgcaat
    10921 caggccgggg acaatgtaat tcttcgattg cagcgagctg ttgtaccaga cgcgcaagcg
    10981 ggcctcgacc ggcgcggaga gcgtcacacc gcccttgcgg ttctgccctt ccgagcgcaa
    11041 ttgcagggag tagttgcgaa gcactgagtc ggcgtagccc agagcgatgg aagccgtgtt
    11101 ggaatcgctg ccgtccagca gcacctgcac gccggcctct gcgccggagc cgatacgccg
    11161 cgaatagccg cgcggaatcg ccaggcccat caggatgcgg ccgctgtcga tgccttgttc
    11221 catggccctg tagttgtcga cgaggccggc gatttggaag aaccgcgatc ctgcaaaacg
    11281 ctggatcagc tcgcggcttt cggcgctttg gtcggagtcg tacaccagcg tcgggatgcg
    11341 gtccacatcg aggctgagag cgtagccgaa cagcaccagg aagagcagcg gcatggccaa
    11401 cgcgatcgcc aggctccgca catcgcgcac aatgtggcgc agctctttct ggaatatggc
    11461 acgcaggcgg cgccggctca cgagctcttt cgctcctcgg cctcaatcag ggccacgaac
    11521 aaatcctcca tagagggctc gattggctcc agacgtcgga tctcgatctg gcgcgccgcc
    11581 agcgcttgcc ggatccgcgc tgaggaagcc tccacatcat ccaccgtcac gtggagcccg
    11641 gcgccgaata ccgccacgtc caaaattccg ggctgcgttt cgatggcccg catgctctcc
    11701 agcggatccg acgaatccag ttgcagcagc cggtgcgatt ccagctcccg cttgagcgtt
    11761 ccgggctctc ccagggcaat gactcggccg gcgtacatca gggcgatgcg gtggcagtat
    11821 tccgcttcgt ccatgtagtg cgtgctgacg aaaatggtgt ggccggttcc cgacagatcg
    11881 cggatcaggt cccaaaacgc cgcgcgggcg attgggtcca ctcccgaggt cggttcgtcc
    11941 agaaatacga tcggtggatc gtgcagaatc gcgcacccca gcgccagccg ctgtttccag
    12001 ccgccggata gcgcggccgt cagggtcttc cgctgctcag cgagctgcgc catctgcagc
    12061 acgtaatcct tgcgctcccg ccgccggtcc ttcgggacgc cgtacatacc cgcgaagaag
    12121 tcgatgttct gctccactgt gagatcgtcg tacagcgaga acttttgcga catgtagccg
    12181 atgttccggc gcacctgttc cggcgcggtc gccacgtcga aacccgccac gcgggcgctg
    12241 ccggatgtag gagtgagcaa cccgcagagc atgcggatgg tcgtcgattt gccggcgccg
    12301 ttgggcccca ggaatccaaa aatttcgccg gcccgcacct cgagtgagac ccggtccact
    12361 gccacaaacg ggccgaacgt cttcaccagg tcgtgaattt ccaccgtcca tccgttcatc
    12421 gcgtttcgag tgcctccgcc tgccgtatgg tctcgataaa gacgccttcc aggttggcgg
    12481 ctcccgcgct gtgtttcagc gcttcgggcg tgtcgcaccg gaccagccgc ccgcgatgca
    12541 tgagccccac gcggttgcag cgctcggctt cgtccaggta tgccgtggtc atcacgatcg
    12601 tgatgccgtc cttcaaaagc tggtagagga tcgtccagaa atcgcgccgc gaaaccgggt
    12661 ctacgccgtt cgtcggctcg tccaggaaca ggatgcgggg ccggtgcagg agcgtgcaca
    12721 tcagagccag cttctgcttc atgccgccgg agagccgcga tgcctgacgc tcgcggaacg
    12781 gctccatacg ggtcatgcgc agaaggcggg cggcaagctc ggcgcgctca ctcccgacga
    12841 tgccgaacag gtcggcgtaa aaatccatgt tctcctgcac ggtgagatcg ccgtacagtc
    12901 cgaaccgctg cgccatgtac ccgatctggt cctttaccgc ctgcggctcg cgggcggagt
    12961 cgcgtccccc cacgtgcgcg ctcccctccg tgggatcgat gagcccgcag agcaggcgca
    13021 gcgtggttgt cttcccggcg ccgtcgggac ccaccagccc gaaaatctcg cccgcgttaa
    13081 cggtgagatc gaggtggtcc accgccgtca ccgccccgaa tcgccgcgtc agcccgcgtg
    13141 tttcgatgag tgcttccatg actaatccag caggatctcc gcatcggccg gcatgttgga
    13201 tttcagttcg tgttgcggat tgtccacttc gattttgatg cggtacacga gcttcacgcg
    13261 ctcctgcttg gtctggatct gcttgggcgt gaattccgct tccgaagcga tgaagctgat
    13321 gcggccgtta taagccttgc ccggataaga gtccgtcgtg atcctggcct tcgagcccag
    13381 cttcacgcgc cccagatcgg tttcgttgat gtatcccctg agccacgggt gatcgatgtc
    13441 gccgatcgtg accaccgtgg cgcccgccgc caggacttcg cccacatcgg cggattttac
    13501 gagcaccacg ccatctaccg gagaaaccgc cgtcgtgtcc gccagctgcg aatcgatgag
    13561 cgccaggttg gccttggccc tggcgacttc ggcccgccgc gtggccagct cctgctcgcg
    13621 gcgtttcatt tcca
//
LOCUS       DASREK010000251         6964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_947107, whole genome shotgun sequence.
ACCESSION   DASREK010000251 DASREK010000000
VERSION     DASREK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6964
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947107"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(468..1016)
                     /locus_tag="VFA54_13590"
     CDS             complement(468..1016)
                     /locus_tag="VFA54_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZS51894.1"
                     /translation="MKRYAKLAARLRVPSGFLAAVLYVIFSNPTPPSLAAGLPVASAG
                     LLLRAWAAGHVAKNEQLATTGPFAYTRNPLYLGTLITATGFAIAARNVWLAIVFGLYF
                     LAVYLPAIQEEENHLRILFPEYQGYAARVPRLRPRLRPARIGAGRFQWRLYIRNREYE
                     ALGGFLAGAALLAAKLHSAVIR"
     gene            complement(1161..3488)
                     /gene="lptD"
                     /locus_tag="VFA54_13595"
     CDS             complement(1161..3488)
                     /gene="lptD"
                     /locus_tag="VFA54_13595"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HZS51895.1"
                     /translation="MPRALAQLAPGAPPAAQAPAAPAANDELFLQADSQSTDGKVWRY
                     LRGHVEVRKGQMVLTADEIDYNEETGDSQARGHVHFSYPVRKEDVYASRAEYNLNSEM
                     GMFYEVHGTVSSASQGNIRILTTNEPFYFQGERAQKIEDHYIVYNGFVTDCKMPRPWW
                     TLGSPRATIVPGQYALMHHTIFRLRKVPLFYTPVYYKSLERLPRQSGFLTPNIGNSSS
                     RGFVLGESFFWAVNRSFDLTPGFTYFSARGLATHVTGRARPSPTSHFDFYFYAMNDRG
                     IKQGSNLVKQGGETLSVNGRAELPLGFHGVINVDYLSSLQFRLAFSETLSEAIGSESH
                     SIGFASKEVSDYFINLALIRDENFFTAEPGDTISVRKLPSIEFNSRDHQLVSGLFPVW
                     LTFDSAADMLSRNQPGFHTAPVSERLDFFPRLSTKLEWKGFRFVPTFGADETYYGQSL
                     RPDGTISGQDLLRSAREISVELAPPSLDRIFDGPKLFADHIKHVIEPKITYTYVTGVS
                     HFDRLIQSGQGNLVSESSDKTSYMYVPGVSDLDRVIRFDERDLLTNTNQIEFSLVNRF
                     LGKTDSNGEVRELASVEIAQRRYFDPTFGGALKPGARNEFLSTVDLTPFAFADSPRNY
                     SPVVSVVRIQPRWNYTIEWRNDYDPLRGRFVNNGITADATVKNFGLSLGEYVVRSDPV
                     LTPNSNQFRGAVRYGSLNKRGWSVGMNLVYDYRVGIMQYANSQVTYNTDCCGFSVEWR
                     RFALGATRNENQFRLALSIANIGSFGNLKKQERVF"
     gene            3659..4732
                     /locus_tag="VFA54_13600"
     CDS             3659..4732
                     /locus_tag="VFA54_13600"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZS51896.1"
                     /translation="MTCGSCGCKNIDEGELRCPRCGRKLSAEGPRAERPPDMVTPPAP
                     PPPAQRASPAAALAPQWRREVSERLQQLQQRRAQRGTGRPDLFSEPPGEDSGEEVPRP
                     DLDQKVLAFEDFAAQRIEPLIVEPPKAAPPEWRKRSRAQARREPETPEPALPSAAATS
                     SRPAEIEAEGEPEADQETAPEREVLCEHPVAPVLLRGMAGALDLAVVTVATGVFFVTF
                     HIMGGAFHFNHKAGTATAAAAAILIAFYFFVYIAYASETPGLQWTGLRVLDYDGEAPR
                     AWQRVTRAVGMILSAAALGLGYLWAFADEEGLTWHDRMSKTFIARDPRYWQRFRYLPR
                     EHSDAGSASPGPSEPGRAPQKRR"
     gene            5038..5223
                     /locus_tag="VFA54_13605"
     CDS             5038..5223
                     /locus_tag="VFA54_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZS51897.1"
                     /translation="MTKLYLTLVRMVREEHGQDLVEYALLAGFIAVAAGALLPGISSS
                     ISTIFSRMASVLSKAAA"
     gene            5548..>6964
                     /locus_tag="VFA54_13610"
     CDS             5548..>6964
                     /locus_tag="VFA54_13610"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZS51898.1"
                     /translation="MKSGLCKKIAAISRAGILGAAIVAVAALWAQSQSPAPVPMPDAA
                     PLAVDRGAAGVWQSLLKLHTRASLLMITAHPDDEDGAMLAYESRGQGARVSLLTLSRG
                     ESGANVMSPDFFDALGLVRTEELLAAGRYYGVDQYFGRVVNFGFSKTKEETLEQWRHD
                     RVLYDAVRVVRLTRPLVVTSVFIGGPSDGHGNHQVAGQMAQEVFKAAADPNMFPDQIQ
                     AGLLPWKPLKIYARTPRARVTAQGVYDYATHHFAPAQVFDYIADKWLPWGLPSNVEIP
                     EGQYDPLLGQSFVQLSREGLGHQKSQNGGTGVPPAGPVNAGYYRFASAVPASDKENSF
                     FEGIDTSLAGIAELAKGGDSGFLKQGLTAVNAQVEEAMKSYSALQPEKVGPYLAAGLK
                     LLNQLLGDVASSGLPAASKYDISHELHIKQEQFQEAIARALGISVLATVTPERESGRG
                     AEFLGAAETFQVAIPGQQFSVV"
BASE COUNT         1450 a         2076 c         2140 g         1298 t
ORIGIN      
        1 atgaagccac tcgaacagct ctgccatgag cttcgccgcc gcaaaaacgg caaatgaagt
       61 aagcagtaaa cgcgtggtct cggagtcggc tacggccagg ttggcgaggg gcatcacgag
      121 cccgggcgct ccagcagggc atacgcccgg tgttgcgggt tggaaaattc gatcttgcaa
      181 caggagccac tcgctccgtg aacctcgagc tcctttgcaa ctccggcggc caattgggga
      241 ccggagcggg atgggagcaa gatgcggcag gacgccgcgg gccaggtgcg caacaattcc
      301 gccaaacggc cggtggccgg cgtcgcctca gcgggcggct tcttccggcg cccgatatgc
      361 gagcgtaagg aatcgaaaat tgacgcctct ggaattccct gcattggctt tatggtaacg
      421 cttgcggccg cgcagcgggc ccagcatcga gcccagcctg agaccaatca gcggatcacg
      481 gccgaatgca gcttggcagc gagcaaagcg gcgcccgcaa gaaacccgcc cagcgcttcg
      541 tactcgcggt tccggatata aagccgccac tgaaacctcc cggcgccgat ccgagccggc
      601 cgcagccggg gccgcaagcg gggcacgcgc gccgcatatc cctgatattc cggaaaaaga
      661 atgcgcaagt ggttttcttc ttcctggatg gcgggcaaat agacggccag aaaatagaga
      721 ccgaagacga tggcgagcca cacattccgc gcggcaatcg caaaccctgt ggccgtaatg
      781 agggtgccca gatatagcgg attccgcgtg taagcgaagg ggcccgtcgt ggcgagctgt
      841 tcgttcttgg ccacatgtcc cgccgcccag gcccgcagca aaaggccagc ggaggcgaca
      901 ggcaggccgg ctgccaggct ggggggagta ggattggaga aaatcacata cagcacggct
      961 gccagaaagc ccgaagggac tcgcaaacgc gcggcaagct tggcgtaacg cttcatcctg
     1021 agatgagtct ttgaaagccg gacgggtcag ccgccagtgc ccgatggcgg cgtccagaga
     1081 aaccggtctg ccgcgaagcc ggcatccctt cccgcggcgc cgtaatcgtt tcctccggcc
     1141 tcaacccatt ctcctcccaa tcagaaaact ctttcctgct tcttcaggtt gccgaacgaa
     1201 ccaatgttcg cgatagaaag agccaaacga aattgattct cattccgggt cgcgcccagg
     1261 gcaaagcggc gccactcgac gctgaatccg cagcaatccg tgttataagt cacctgcgag
     1321 ttcgcgtact gcataatgcc gacccggtag tcatacacca ggttcatccc gacgctccag
     1381 ccgcgtttat tcagcgagcc ataacggacg gcgccccgaa actggttgct gttcggggta
     1441 agcacgggat cggaccgcac cacgtactcg cccaaagaca gcccgaagtt cttcacggtc
     1501 gcgtcggcgg taatgccgtt attcacgaac cggccgcgca gaggatcgta atcgttgcgc
     1561 cattctatcg tgtagttcca gcgcggctgg attcgcacga cgctcacaac cggagaatag
     1621 tttcgcgggc tgtcggcgaa agcgaaaggc gtcaaatcaa cagtggagag aaactcgttg
     1681 cgggcgcccg gcttgagggc gccgccgaaa gtcggatcga agtaccgccg ctgcgcaatt
     1741 tccacacttg ccaattcgcg gacttcgccg ttggagtcgg tcttgcccaa gaaccggttg
     1801 accagcgaaa actcgatctg gtttgtgttg gtaagcagat cccgctcgtc gaatcgaatc
     1861 acgcggtcga gatcggaaac gccgggtaca tacatatacg aggtcttatc gctgctttcg
     1921 ctgaccaaat tcccttgacc ggattgaatc aagcggtcga aatgagagac gccggtcaca
     1981 taggtgtagg tgatcttagg ctcgatgacg tgcttgatgt ggtcggcgaa taatttgggg
     2041 ccgtcaaaaa tcctgtccag agaaggcggc gccagctcta cggaaatttc ccgggccgac
     2101 cgcagcaaat cctgtccgga gatcgtgcca tccggcctga ggctctggcc gtaatacgtt
     2161 tcatccgcgc cgaatgtcgg gacgaagcgg aagcctttcc actcgagttt cgtcgagagc
     2221 ctggggaaaa aatcgaggcg ctcggagacg ggcgcggtat gaaaaccggg ctgattacgg
     2281 ctcagcatgt ccgctgccga gtcgaacgta agccacacgg gaaacaaacc tgagacgagc
     2341 tgatgatccc ggctgttgaa ttctatgctg gggagcttgc gaacggaaat cgtatcgccg
     2401 ggctcggccg taaaaaagtt ctcgtcgcga atgagcgcca aattgatgaa gtagtcggat
     2461 acttccttgc tggcgaatcc gatggaatga gattccgagc cgatcgcttc gctcagagtc
     2521 tcgctgaatg ccagccggaa ttgcagtgaa ctgagataat cgacattgat gaccccgtga
     2581 aagcccaagg gcagttcggc ccggccgttg acggaaagcg tctctccccc ttgctttacc
     2641 aggttgctcc cctgcttgat gccccggtca ttcatcgcgt aaaaatagaa atcgaaatgc
     2701 gaggtcgggc ttgggcgggc tcgccccgtt acgtgggtgg ctaggccccg ggcgctgaag
     2761 taagtgaaac ccggcgtgag gtcgaagctg cggttcacgg cccagaaaaa cgattccccg
     2821 agcacgaagc cccggcttga gctgttgccg atattgggcg ttaaaaatcc gctctgcctg
     2881 ggcagccgtt ccagcgattt gtaatacacc ggcgtataga aaagcggcac tttgcgaagg
     2941 cgaaagatcg tgtggtgcat caaggcatac tggccgggaa cgatggtcgc cctcgggctt
     3001 cccagagtcc accagggccg cggcatcttg caatcggtca caaacccgtt gtagacgatg
     3061 tagtgatctt cgatcttttg ggcccgctct ccctggaaat agaaaggctc gttggtggta
     3121 aggatccgga tgttgccctg gctcgcagaa ctgaccgtgc cgtgaacttc ataaaacatc
     3181 cccatttccg agttgaggtt gtattcggcg cgggaggcgt aaacatcctc tttccggacc
     3241 ggatatgaaa aatgcacgtg gccccgcgct tgggaatcgc ccgtctcctc gttgtaatcg
     3301 atttcatcgg cggtcagaac catctggcct tttcttacct ccacatgacc gcgcaagtag
     3361 cgccagactt tcccatcggt cgattgggag tcagcctgga gaaacagctc atcattcgca
     3421 gcaggcgcgg cgggcgcttg cgccgccggc ggcgcgcccg gagcaagctg agcgagcgcc
     3481 cgcggcacag aaaacaagtg acaaagggca accaatgtga taggtaggat gagaacacac
     3541 gaaacggcgc tgcgaggcag cataaaagag aggaaactct gaacgtaaca cgcgcaaaaa
     3601 cgaaaccgca accgggcggg aagctccccc agacaagccg gccggagacg gcggaacgat
     3661 gacttgcggc tcctgcggct gcaaaaacat tgacgaaggg gaattgcgct gcccgcgctg
     3721 cggccggaag ctttcagccg aagggccgcg ggccgagcgg ccgccggata tggtcacgcc
     3781 gccagcgccc cctcccccgg ctcaacgggc cagcccggcg gcggcgttgg ccccgcaatg
     3841 gcggcgggag gtttccgaac gcttgcagca actccagcag aggcgggcgc agcgcggtac
     3901 cggacggccc gatctttttt cagaaccgcc cggagaagat tccggcgaag aggttccccg
     3961 tccggatctg gaccagaaag ttttggcctt tgaagacttc gccgcccagc gaatcgagcc
     4021 tttgattgtc gagccgccca aggccgcgcc tccggaatgg cgaaagcgct cccgcgcaca
     4081 ggcgcggaga gagccggaga cgccggaacc ggccttacct tccgctgccg ccacgtccag
     4141 caggccggcg gagatcgagg cggagggcga acccgaggcg gatcaggaga cagcgccgga
     4201 acgggaagtg ctctgcgagc accccgtcgc ccctgtgctg ctgcgcggca tggcgggagc
     4261 gttggacctg gccgtggtga cggtcgcaac gggcgtcttt ttcgtgacgt ttcacattat
     4321 gggcggcgcc ttccacttca accacaaggc gggaaccgcc accgccgcgg cggccgccat
     4381 actcatcgcc ttctattttt tcgtctatat tgcctacgcc tcagaaacgc ccggattgca
     4441 gtggaccggt cttcgcgttc tcgattacga tggcgaagcg ccgcgggctt ggcagcgcgt
     4501 cacccgggca gtcggcatga tcctgagcgc cgccgccctc ggtcttggct atctctgggc
     4561 gttcgccgac gaagagggct tgacctggca cgaccgaatg tctaaaacat ttatcgcccg
     4621 ggacccgcga tactggcagc gctttcgcta cttgccacgg gagcattcgg acgccggaag
     4681 cgcatcaccg ggcccgtcgg aaccaggaag ggcgccgcaa aaacgccggt gactgcgcgg
     4741 ttccggccgc atttcgaaag acacaggcct tccattttcg gctggcgggc ggagcttccc
     4801 gccacgcggt tactctccgg cttagcattc aaaatccatg ctgccttgcc gtacgggtga
     4861 ggccggctgc ggctaagttt tcaaccctag tgaaaccgga cgaaagatag cgcccgcggc
     4921 cgctggaaga tagctagctg gcttcggccc gccggcgtat tctcatcttg catctgtgga
     4981 atcccgaagc tctggacgct tcggcgctcc aaagacaaaa ttgaaaagga gaagtacatg
     5041 acgaaacttt acttgacgct ggtccggatg gttcgtgagg aacacggcca ggacctggtg
     5101 gaatatgccc ttctggcggg ctttattgcg gtcgcggccg gcgccttgct gcctggcatc
     5161 tcgagcagca tcagcacgat cttcagccgc atggcgtcgg tgctttcgaa agcagccgcg
     5221 taaacggagc gcgcgcggcc cgatccatta gcttcagcct gatttgaaat cgaggacggc
     5281 gtccactggc agacacgtcc tcgcccaggc gaccatcggc ccctctctcc accctgccgg
     5341 gccttcgggt tcggcaggga gcccttccat taattgatcc taattgatct aattgatcct
     5401 aattgatccg gaaaattcat cccggaaaac attcctggac cgctctgttt ggccgaactc
     5461 ccaagagccg atggcgccgt ttgacatgac cgggggcggt gaatatgatt cttgcaaggc
     5521 gctccggccg gaattcggga ggctgctatg aaatcgggat tgtgtaaaaa aattgccgcc
     5581 atcagtcgtg ctggcatcct gggcgctgcg atcgtcgctg tagccgcttt gtgggcccaa
     5641 tcgcaatcgc cggcgccggt acctatgccc gacgccgcac ccctggccgt agatcgggga
     5701 gccgccgggg tttggcaaag cctgttgaaa ctgcacacac gcgcgagctt gctgatgatc
     5761 accgcgcatc ccgatgacga agacggcgcc atgctcgcat acgaatctcg aggccagggt
     5821 gcgcgggtct ctctgcttac actgtcccgc ggggagagcg gggcgaatgt catgtcccct
     5881 gatttcttcg acgccctcgg tctggttcgg accgaagagc tgctcgcggc cggccgctac
     5941 tatggcgtgg accagtattt cggccgggtg gtcaatttcg gtttctccaa gaccaaggaa
     6001 gagacgctgg agcagtggcg gcatgaccgg gtgctatacg acgcggtgcg ggtagtgcgc
     6061 ctgacccgcc cccttgtcgt cacctccgtc ttcattggag ggcccagcga cgggcacggc
     6121 aatcatcaag tagcgggcca aatggcgcag gaggttttca aggcggccgc cgaccccaac
     6181 atgtttccag atcagatcca ggccggactc cttccgtgga agcctttgaa aatatatgca
     6241 agaacgcccc gggcgcgcgt tacggcccag ggagtttatg actatgctac acatcatttc
     6301 gcgccggcgc aggtattcga ctacatagct gacaaatggc tgccctgggg actgcccagc
     6361 aatgtcgaga tcccagaagg gcagtacgac ccgttgctcg gccagtcgtt tgttcagctt
     6421 tcgcgcgagg ggctgggcca tcagaaatcg cagaacggcg gcacaggcgt gcctccggcc
     6481 gggccggtga acgctggata ttaccgcttc gcttccgccg ttcccgcttc cgataaagag
     6541 aattcctttt ttgagggaat tgacacgtct ctggcgggca tcgcggagtt ggccaaagga
     6601 ggcgattcgg gattcctgaa gcagggcttg accgccgtca acgcccaggt tgaggaggcg
     6661 atgaaaagct attcggcctt gcagccggag aaggtcggtc cctatctggc cgccgggttg
     6721 aaattgctca atcagctcct gggcgacgtc gcttcgagcg gccttcccgc ggcatcaaaa
     6781 tatgacatca gccatgaact gcacatcaag caagagcaat tccaggaggc gatcgcgcgc
     6841 gccctgggaa tttccgtgct ggctacggta acgccggaac gcgagagcgg gcgcggtgcg
     6901 gagttcctgg gcgcggccga gaccttccag gtagcgattc ccggacagca gttctcggtg
     6961 gtcg
//
LOCUS       DASREK010000252         4949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1600137, whole genome shotgun sequence.
ACCESSION   DASREK010000252 DASREK010000000
VERSION     DASREK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600137"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFA54_13615"
     CDS             <1..802
                     /locus_tag="VFA54_13615"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZS51899.1"
                     /translation="GIIGKYVEYEDSYKSLKEALLHGGLAHNLRVNLDWVEAEGISQS
                     QERGEGWRRQLEPYDGILVPGGFGKRGIAGMIQAIRYAREEKVPYFGICLGMQTMVIE
                     FARHVCGLEGADSSEFNPATEHRVIYKLRELKGIDDLGGTMRLGAWPCLLQPGSLAQR
                     AYGKMEISERHRHRYEFNREYEETLTAGGLRISGETPDKTYVEICELPDHPWFLGCQF
                     HPEFQSRPLEPHPLFSAFIAASYENRRRRRQARRMAEEEQFLRVGRNQ"
     gene            918..1745
                     /gene="kdsA"
                     /locus_tag="VFA54_13620"
     CDS             918..1745
                     /gene="kdsA"
                     /locus_tag="VFA54_13620"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZS51900.1"
                     /translation="MEIGQVKIGAGSPLCFIAGPCVIESAEHAIGLATRLSTIANERR
                     APLIFKASYDKANRTSASSFRGPGLREGLRVLAEIKAKTGLPVLTDVHEAAQVGPAAE
                     VCDVLQIPAFLCRQTDLLLEAGRSARAVNIKKGQFLSPWDMEHAVEKVTSTGNHRVIL
                     TERGASFGYNNLVVDMRSLIVLRKFGFPVVLDVTHGVQLPGGAGRASGGQPEFIEPLA
                     RAGVAAGVDGIFLEVHEQPERALSDGANALRLDLLPGLLDRLHRIDALVKPWLDDSA"
     gene            complement(1799..2314)
                     /locus_tag="VFA54_13625"
     CDS             complement(1799..2314)
                     /locus_tag="VFA54_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51901.1"
                     /translation="MRVDWESPLAGSTTKKVLVRRFDGQELAGYASPATFLRPAGVEV
                     LTRDGQAQLVAYKDLKAVYFVREFVPPPGAEEKKLFQSRPKLEGLWIRMRFRDGDMLE
                     GILPNDLLQLMDKGVTITPPDPYSNNQRIFVPRLALSELAVLGVIGSPLTKPRRKAQP
                     AGKQQIELFGE"
     gene            2446..3363
                     /gene="xerD"
                     /locus_tag="VFA54_13630"
     CDS             2446..3363
                     /gene="xerD"
                     /locus_tag="VFA54_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZS51902.1"
                     /translation="MATRAECKPRPVLHGRIIAFLNFCRVEKGLAANTLDAYTRDLAA
                     LQKFCVQQNWPLESLTAEQLRRFLDSRYQARLSSRSIARLLASIRNFYSFLLREGVVP
                     SDPSADIAVPRQWKKLPHFLSADEIALLLKAPDSGHPLGLRDRAMLELLYATGLRVSE
                     LVRVRTPDVNTELGVLRTLGKGGKHRLIPVGRAALAALDAYIPARRRLLGRKVSEYLF
                     LTARGRPLTRQSFWHRLRHYGRVAGIRRVLTPRVLRHSFATHLLERGADLRSVQLMLG
                     HADISTTQIYTHVVRDRLRQVYDQHHPRA"
     gene            3367..4641
                     /locus_tag="VFA54_13635"
     CDS             3367..4641
                     /locus_tag="VFA54_13635"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51903.1"
                     /translation="MSDYIFLLESRLNKDQLQLLAQVQRAAERAQVHLFLAGGAIRDL
                     LGGFPIRDLDFSVEGPALKLVRQLDSRLFSILSTDQHRQSAELLFGGAATAEIAMCRK
                     EAYSKTGGQPEVTRATIQDDLMRRDFSINALALSLNPASRGLLLDPTNGLADIEHKEL
                     RALSNYSFFDDPSRLLRLVRFETRLKYSMAEKTKTQFASAREAEVEEYIPPRVRLHEL
                     RQISAEPESAEVVKALNAAGLLKVFEPHLGKKLDLALLAKLDKARKLIEESGVRPDSF
                     APFLYCLTKKLNTAERKNLRTRTGMRAAEASAWMDLETRAKSLQKALASKQATPNSRL
                     YRLLSSQDISLILFLLAFSPLQVVRERIKTYFTQLRPIAQTLDDQEVEALGVKRSSPK
                     FAAQREAYLMARLDKKPVAKSEAAKTASGPGR"
BASE COUNT          959 a         1515 c         1496 g          979 t
ORIGIN      
        1 cggcataatc ggaaagtacg tggagtatga agacagctac aagagtctga aggaagcttt
       61 gctgcacggg ggcctggccc acaatctgcg ggtcaacctg gactgggtgg aagccgaagg
      121 catctcccag tcccaggagc ggggtgaggg ctggcggcgg caactggagc cttacgacgg
      181 cattctggtt cccggcggat tcggcaaacg cggcatcgcc ggtatgatcc aggcgatccg
      241 ctatgctcgt gaagagaagg ttccctattt cggcatctgc ctgggtatgc agaccatggt
      301 gatcgagttc gcgcgccatg tctgcggcct cgaaggggcc gattcgagcg agttcaaccc
      361 ggcgaccgag caccgcgtca tctataagct gagagaactc aaaggcatcg acgacctagg
      421 cggcaccatg cgcttgggag cgtggccttg cctgctccag ccgggcagtc tggcccagcg
      481 agcgtacggg aagatggaaa tcagcgagcg ccatcgccac cggtacgaat tcaaccgcga
      541 gtatgaggaa acccttacgg cgggcggcct gcggatatca ggggaaaccc ccgacaagac
      601 gtacgtcgaa atttgtgaac tgcccgatca tccctggttc ttgggctgtc agtttcaccc
      661 cgagtttcag tccagaccgc tggagccgca cccgctcttt tccgcattca tcgctgccag
      721 ctacgagaac cggcggcggc gccggcaggc ccgaaggatg gccgaggagg agcagtttct
      781 ccgcgtcggc cgcaatcagt agcgaatttt tttgccagtg acaagcaatt tggctgaaaa
      841 aggcggcgtc ccgcagggcc ggccagagcc acgctccggg cggagctgcc ttccacggcg
      901 caaaacatga acctctcatg gaaatcggtc aggtgaagat cggcgcgggc tctccgttgt
      961 gcttcattgc gggcccttgt gtgatcgaaa gcgcggagca cgcgatcggc ttggctaccc
     1021 gtcttagcac gatcgccaac gaacgccggg ctccgctgat cttcaaggct tcttacgaca
     1081 aggccaatcg taccagcgcg agttcctttc gcggccccgg tctccgggaa ggcctccggg
     1141 tgctggctga aatcaaagca aaaacgggcc tgcccgttct gaccgatgtc cacgaagcgg
     1201 cgcaggtggg acccgcagcg gaggtttgcg acgttttgca aatccccgcg tttctttgcc
     1261 ggcaaaccga tctgctgctg gaagcaggcc gcagcgcgcg tgcggtgaac ataaaaaagg
     1321 gccagttcct ttcgccctgg gatatggaac atgccgtaga aaaagtcacc tcaaccggaa
     1381 accatcgcgt gatcctgacc gagcggggcg cgagtttcgg ctacaacaat ctcgtcgtcg
     1441 atatgcggtc tctcatagta ctgagaaaat tcgggttccc cgtcgtcctg gacgtcacgc
     1501 atggggtgca actgcccgga ggggcgggcc gggcgagcgg gggccagccg gagttcattg
     1561 agccgctcgc gcgcgccggc gttgccgcgg gcgtcgatgg catcttcctc gaggttcatg
     1621 agcaaccgga gcgggcgctc tccgacggcg ctaacgcctt gcggctggat ctcctgcccg
     1681 gtctcctgga ccggctgcac cggattgacg ccttggtcaa gccctggttg gatgattccg
     1741 cgtagcctct ctgtccgcgg aaagtccggc gcgcgcggcg ggatccggcg gcaggcgcct
     1801 attctccgaa gagctcgatt tgttgtttgc ctgccggctg ggctttgcgc ctcggcttcg
     1861 tcaacggact gccgatgact cccagcacgg ccagctccga gagcgccagg cgcggcacga
     1921 aaattcgctg gttgttggaa taggggtccg gcggagtgat ggtcacaccc ttgtccatca
     1981 gttggagcag gtcgttgggc aagatgcctt cgagcatgtc gccatcccgg aatcgcatcc
     2041 gaatccagag gccctctaat ttaggccggg attgaaacaa tttcttttct tcggcgcctg
     2101 gtggcggaac aaactcgcgg acaaagtaga cggctttcag atccttgtag gcgacaagct
     2161 gggcttgccc gtcccgcgta aggacctcaa cgccggctgg acgcaaaaag gtcgccggac
     2221 tggcatagcc tgccagttct tggccgtcga agcggcggac cagcactttc tttgtagtcg
     2281 atcccgccaa aggcgactcc cagtctaccc tcaattacgc caacgcgttg tgattgtttc
     2341 gtttgccagc gggtttccgg tatgctaata tgcgccggag gcagtgtcaa gcctcgccgc
     2401 gattgtaaat cactgattct tcggcgcttg ctttaggaag gaatcttggc gactcgcgcg
     2461 gagtgtaagc cacggccggt actgcatggg cgcatcattg catttctaaa tttttgccga
     2521 gtcgagaagg gcttggcggc aaacacttta gatgcctaca cgcgtgattt ggcggcgctc
     2581 caaaaatttt gcgtgcagca gaattggccg ctggagagcc tcactgccga gcagttgcgc
     2641 cgattcctgg attcccgcta tcaagcccgg ctcagcagcc ggtctatcgc ccgtctgctg
     2701 gccagcatcc gcaattttta ctcgtttctg ctcagagagg gtgtcgtgcc ctcggacccg
     2761 tcggctgaca ttgccgttcc tcgccagtgg aaaaagctgc cccactttct gagcgccgat
     2821 gaaatcgccc tcctgctgaa ggcgcctgac tcgggccatc cgctgggcct gcgcgaccga
     2881 gctatgctcg agctgctgta cgcgacaggc ctgcgagtct cggaactggt gcgcgttcgc
     2941 acccctgacg tcaacacgga actgggcgtc cttcgtacgc tcggcaaagg cggcaagcac
     3001 cgcctgattc ccgtcggacg tgcggctctt gcggcattag acgcgtatat tcccgcgcgg
     3061 cggcggctgc tcggccgcaa agtttccgag tatctcttcc tgacggcacg aggccgtccc
     3121 ctgacacggc agtccttctg gcaccggctc cggcattacg gccgcgtggc gggcatccgc
     3181 agagtcctga ccccgcgtgt gctgcggcat agctttgcga cgcatctttt agagcgcggg
     3241 gcggacttgc gcagcgtgca gctcatgctg ggacacgctg atatttccac aacccagatc
     3301 tatacacacg tcgtgcggga ccggctgcgc caggtgtacg accagcacca tccccgggcc
     3361 tgatccatga gcgactacat atttttgctg gagagccggc tcaacaagga ccagttgcag
     3421 ttgttggcac aggtgcagcg cgccgccgaa cgggcgcaag tgcatctctt cctggccggc
     3481 ggcgccatcc gcgacctgct cggaggcttt cccatccggg atctggattt ctcggtcgaa
     3541 ggaccggccc tgaaactggt ccggcaactc gattcccggc tgttttcgat tctctccacc
     3601 gatcagcatc gccagtccgc agaactactg ttcggcgggg ccgccaccgc cgaaatcgcc
     3661 atgtgccgca aagaggcata cagtaagaca ggaggacagc ccgaagtcac gcgcgcgacg
     3721 attcaagatg acctgatgcg ccgcgatttc tccatcaatg cgttggccct ttccctcaat
     3781 ccggcttcgc gcgggctgct gctggatcca accaacggct tggcggatat cgagcacaag
     3841 gaattgcggg cgctcagcaa ctacagcttc ttcgacgacc ccagccggct gctccggctg
     3901 gtccggttcg aaacgcggct gaaatattcc atggccgaaa agaccaagac ccagtttgcc
     3961 tcggcccgcg aagcggaagt ggaggagtat atcccgcctc gagtgcgcct ccatgaattg
     4021 cggcagatct cggcggaacc ggagagtgca gaggtggtca aggcgctgaa tgccgccggc
     4081 ttgctgaagg tttttgagcc gcatttgggc aaaaagctcg atctcgcgct gctggcgaag
     4141 ctcgacaaag cccgcaagct gattgaggaa tccggcgtcc gcccggacag cttcgcgcct
     4201 ttcctgtatt gcctgacgaa gaagctcaac accgcggaac gcaaaaatct gcgtacaaga
     4261 acaggcatgc gcgccgcgga agcctctgcc tggatggatc ttgaaacgcg cgccaaatcc
     4321 ctccagaagg cgcttgcatc caagcaggct acgccaaact cccgtctcta ccgccttttg
     4381 tcttcccagg acatttcgct gatcctgttc cttttagcct tttctccatt gcaggtggtc
     4441 cgcgaaagaa tcaagacgta tttcacccag cttcggccga tcgcgcaaac tctcgacgac
     4501 caagaagttg aagctctcgg cgtgaaacgc agctctccga aattcgcggc ccagagggaa
     4561 gcgtatctga tggctcgctt ggacaaaaag cccgtcgcca aaagtgaagc tgccaaaacg
     4621 gcttccggac ccggccgcta gggcggcgtg gccgcctggg caggcgcggg aatcgatcaa
     4681 aattccacaa accgcgctag ggggcagatg accgctgctc tgaagtggct aaactgctcg
     4741 ccttggagac agcgattccc gctcgaaaag ccggctgccg cgggctcgcc aagctgcggg
     4801 cgctgcctca tttgcgccag ccggttggag agtgctagag tggtagcgag cgcaacggtt
     4861 ccgccgccga gggctcccca atttgacaaa cgcggtcccc gccccctatg attttttctt
     4921 aagagtttgt tgggcatcac tccactact
//
LOCUS       DASREK010000253         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_615878, whole genome shotgun sequence.
ACCESSION   DASREK010000253 DASREK010000000
VERSION     DASREK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615878"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFA54_13640"
     CDS             complement(<1..202)
                     /locus_tag="VFA54_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS51904.1"
                     /translation="MVATTEIAPEIYGLAVFVPEINLEFSHFLVRDDEPLLFHAGLRA
                     MFPLLRKAISRLIDLSRISPDSA"
     gene            346..960
                     /locus_tag="VFA54_13645"
     CDS             346..960
                     /locus_tag="VFA54_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51905.1"
                     /translation="MSTRTFLAGLLGGVTMFVWMFIAHMVLPLGEAGVKEMPNESPVL
                     SSMQASMGAGSGVYVFPGTGLPPNATADQRNAAMRDYNGKLAASPSGFLVYHGPGATF
                     SFPKLLGTEFALEVAEALLAAWLLSMTVLKGLMARAGFVTVVGVIAAIATNVSYWNWY
                     GFSGTYTAAYVFTQAAGFLCAGLVMAALVRGKGGLPARAVSGGR"
     gene            complement(944..1636)
                     /locus_tag="VFA54_13650"
     CDS             complement(944..1636)
                     /locus_tag="VFA54_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51906.1"
                     /translation="MLPEKLRSIFRRESEPASPGSSRPAGGGFPLRRSPRKEVTVTRQ
                     SRGLEQFFFNVRDIVGLSILDLAGASQENINFLTNLGHKVYSQDIIRSLDEAFGPDPA
                     EQTNVGRIEYFLRTNFDYPPGTFDGALLWDSLQFMGPALLNATVERLYEVMNPKSYLL
                     AFFSSSDRAAEAPSHIFRIVDNKTMLITERDIRPTGQVFNNRNLEKVFAKFESVKFFL
                     TRESLREVIVRR"
     gene            complement(1647..2114)
                     /locus_tag="VFA54_13655"
     CDS             complement(1647..2114)
                     /locus_tag="VFA54_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZS51907.1"
                     /translation="MSSTPYRMPDPEPRNTATIGKAVKINGQIFTKEDLYVDGDVEGT
                     IESHDNKVTIGPNGRVQAGIRAREVVILGQVQGNVEASDKVDIRKDAKLVGDITTSRI
                     SIEDGALFKGSIDIKKAEPKPAAAPAPAVAPPRPAEPVAVPAASTAGAAGMKR"
     gene            complement(2490..>2801)
                     /locus_tag="VFA54_13660"
     CDS             complement(2490..>2801)
                     /locus_tag="VFA54_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZS51908.1"
                     /translation="VDFMMRYELGLEAPDPKRAGRSAGTIFASYIAGGLVPLAPYFFA
                     SGVESALVVSVALTAVALFIFGAIKGRFTGIAPARGGFETTLIGGAAAAAAFLMARLF
                     R"
BASE COUNT          536 a          790 c          880 g          595 t
ORIGIN      
        1 tggcggagtc tggagagatc ctggagagat caatgagacg tgagatcgcc ttgcgtaaca
       61 gcgggaacat agctcgcaat ccagcatgaa atagcagcgg ctcgtcgtcc cggacgagga
      121 aatggctgaa ctccagattg atctcgggaa cgaagaccgc aaggccgtag atctcaggcg
      181 caatttcagt ggtagcgacc acacactctc cttttttgat cttacataca tggctgtagt
      241 gtcccgggcc ctgtgggcgg ccagcttact atccgcaaaa tgcattatcc tgtcgagagt
      301 cagccagggt gactgcacgg attcagcgga acgaaggagg cggggatgag tacgcgtacg
      361 tttttggccg ggttactggg cggcgtgacg atgttcgtct ggatgttcat tgcccatatg
      421 gttctacctt tgggcgaagc gggcgtgaaa gagatgccaa atgaatcgcc tgtactgagt
      481 tcgatgcagg cttcgatggg cgccgggtcc ggcgtctatg tgttcccggg aacggggttg
      541 ccgccgaacg cgacggccga tcaaaggaat gcggcgatgc gcgattataa cggcaagctt
      601 gcagcttcgc cctcgggatt tctggtttat cacgggccgg gcgcgacgtt cagtttcccg
      661 aaattgctcg gtaccgagtt tgcgctggag gtggcggaag cgcttctggc ggcgtggctg
      721 ttgagcatga cagtcctgaa aggtctcatg gcgcgggcgg ggttcgtgac ggttgtcggg
      781 gtgattgccg cgatcgcgac gaatgtctct tactggaact ggtacggctt ttcgggaacc
      841 tacacggccg cctacgtgtt cacgcaggcc gcgggatttt tatgcgccgg cctggtaatg
      901 gcggcgttgg tgcgcgggaa agggggccta ccggcccggg ctgtcagcgg cggacgatga
      961 cttcgcgcag gctctcccgc gtgaggaaga atttcaccga ttcgaatttc gcgaacacct
     1021 tctcgagatt gcgattgttg aacacctgac cggtggggcg gatgtcccgc tcggtaatca
     1081 gcatcgtctt gttgtcgaca atgcggaaga tgtgggaggg cgcctcggcg gcccggtcgc
     1141 tcgaggagaa aaacgcgagc agataagact tcggattcat cacctcgtac agccgttcta
     1201 cagtagcgtt cagcagcgcc gggcccatga actggaggga atcccagagc agcgcgccat
     1261 cgaacgtgcc cggcgggtaa tcgaagttgg tgcgaaggaa atactcgatg cggccgacat
     1321 tggtctgctc ggcgggatcc gggccgaacg cctcatcgag gctgcgaatg atgtcctggc
     1381 tgtagacctt gtggccaaga ttggtcagga agttgatgtt ttcctggctt gcgccggcga
     1441 ggtcgagaat cgacaaacca acgatgtcgc ggacattgaa aaagaactgc tcgaggccgc
     1501 ggctctgccg tgtgacagtg acttctttcc tgggggaccg gcggagcggg aagccgccgc
     1561 cagcggggcg ggaactaccc ggagaagccg gttcgctctc ccgccggaag atggaacgga
     1621 gcttctccgg taacaacccg acctcttcag cgcttcattc ccgcggcgcc ggcagtactg
     1681 gctgcgggaa ctgcgaccgg ctccgcggga cgcggaggcg ctacggccgg agcaggcgcg
     1741 gcggccggct tcggttccgc tttcttgata tcgatgctgc ccttgaacag agcgccatct
     1801 tcgatgctga tacgtgatgt agtaatgtct cctaccagct ttgcatcctt gcggatatcg
     1861 actttatcgc tggcttcaac gttgccctgc acctggccaa gaatcacaac ctcacgggcg
     1921 cggatgccgg cctgaacacg gccgttgggt ccgatcgtga ccttgttgtc gtgcgactcg
     1981 atcgttcctt ccacgtctcc gtctacgtac agatcctcct tggtgaagat ctggccgttg
     2041 atcttgactg cttttccgat ggtcgctgta ttgcgaggct cgggatcggg catcctgtag
     2101 ggcgtagatg acactggggt gggctccttc ctggtctctg atgcagttgg tgcgggagcc
     2161 ggtggcgcgc tggccggtcg cggcgccacg ggctcttcct ttttattcca catgctcact
     2221 tcttaacggc ctccaaaata ggttcctgtt gacgattgcg tatattgtca gcgctcaatt
     2281 cagtctatcc gcacaccgga agaacgcgca attgcgagtt cgcctatctt tgcgcgcgac
     2341 tgacgcccaa cactgtctct tcattagagc accgcggccc atcgttacct gatttgcgca
     2401 gactcgccct gagccgacaa tcatatctgc tcccgccgcg caccagcctt caaacgcgca
     2461 caacgcccgc tcgagtagcg ggctgtgcgc tagcgaaaaa gccgggccat cagaaacgcc
     2521 gctgcagcgg cggcgccgcc gatcagcgtg gtctcaaatc cccctcgcgc cggcgcaatt
     2581 ccggtgaatc tccccttgat tgctccaaat ataaatagtg ccacggcggt gagcgcgacg
     2641 gaaacaacaa gcgcgctttc cacgccggat gcgaaaaaat atggcgcgag cggcacgagt
     2701 ccgcctgcaa tgtaagaagc gaagatggtg ccggcgcttc ggccggcgcg tttcgggtct
     2761 ggcgcttcaa gcccgagctc ataccgcatc atgaaatcga c
//
LOCUS       DASREK010000254        19627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_947944, whole genome shotgun sequence.
ACCESSION   DASREK010000254 DASREK010000000
VERSION     DASREK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 19627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19627
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947944"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..599)
                     /locus_tag="VFA54_13665"
     CDS             complement(63..599)
                     /locus_tag="VFA54_13665"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HZS51909.1"
                     /translation="MGVRLHRRWCVVPEWAWVPVASGALVMIIGLIGILARRPLLFAS
                     LGPTAYLESEPRLPTSRFYNVLVGHLIGLGAGFLAVLTVGAAQAPPVLAAKDLTMPRV
                     WAATFALGLTVFFSLIARAPHPPAGATTILVAIGAFRTLEDAVDVFIGVLAIAVLGEG
                     VRWLRLRRLLPGSREARR"
     gene            complement(695..931)
                     /locus_tag="VFA54_13670"
     CDS             complement(695..931)
                     /locus_tag="VFA54_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51910.1"
                     /translation="MDKPVGVELAWIKPQDHVLGIGFGPGAEIEHVANVATCGFVVGN
                     HGGVSRFRPNLSPAPKDTFPGQEIFPGAGNLRNR"
     gene            1052..1432
                     /locus_tag="VFA54_13675"
     CDS             1052..1432
                     /locus_tag="VFA54_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51911.1"
                     /translation="MVHELTAIPARIDFRKFDREFALTNRLRTEGTDNLLTAAYAAGV
                     RRFIAQSYAGWPYARQGGPVKTEEDPLDPNPPSGVRKTLEAIRYLESAVLGSRKIQGV
                     VLRYAARLSARVRPGTSPASLPGS"
     gene            complement(1885..2523)
                     /locus_tag="VFA54_13680"
     CDS             complement(1885..2523)
                     /locus_tag="VFA54_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51912.1"
                     /translation="MRGGFSGGRIGGGFAGGSFIGGGSFHGGFVRSGAFRGGFVRSGV
                     FRGGFHPGFSRFGFRRSRVFFSVGLGFYPYSFAYWPFAYDYWWPYGYGYPYYYYPQYY
                     YPPYDYSEPYPNVVGQQAVPAPGGAVQTVPQGYWLIALKDNTIIAATDYWLDGSTLHY
                     VTRDGNNLTIPLDRVDLPFTRELNAERGAQFRLPRPSDTQQPARRDSYGRAY"
     gene            2896..3210
                     /locus_tag="VFA54_13685"
     CDS             2896..3210
                     /locus_tag="VFA54_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HZS51913.1"
                     /translation="MDDKRLSYFLFGLGMGVGIGILFAPKSGEETREILKGKAGEGRE
                     FLRRKTEEIRDSTTEILNKGREAFQSQKENLAAALEAGKQAYRQSVSADQPRSSEGPA
                     GM"
     gene            3258..3758
                     /locus_tag="VFA54_13690"
     CDS             3258..3758
                     /locus_tag="VFA54_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51914.1"
                     /translation="MDTRFLLIISIFTGVAAVALLVQMFVLVGLYLTAKATQARITSL
                     ADRVEPLIDTSRRMAEETRQQAREILGKLTQITEATRVQVLRIDEMLAEVSVHTRSNL
                     ELVDRAVAATAAKIEETVNRWQRTILAPVRGINGAAAAVRAVVSHLGRRRSADGRPTQ
                     EEDLFI"
     gene            3968..5092
                     /locus_tag="VFA54_13695"
     CDS             3968..5092
                     /locus_tag="VFA54_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS51915.1"
                     /translation="MPIRVALLGTKFMGKAHSQAYRSVNMYFPDAPQVEMKVIVGRNA
                     QETETARALYGWQEASTDWQKTVARADVDLVDVSTPGNLHCEMVLAAAKAGKHVVCEK
                     PLANTLAEAERMLEAVEKAKVRHLLMHNYRRAPAVAMAKKLVEEGRLGKLYHFRARYL
                     QDWAMSPDLPMLWRFVAQSAGSGALGDLGAHIVDLARYLCGEITEVAAAMETFIKQRP
                     VAPGSSRKAKVTVDDAAAMVVRFKNGALGTLEASRFAPGRKNQNTFEINGSKGSVEFD
                     LEDLNVLKFFSTEDPPDVQGFHKVLCLAKGVHPYASAWWADGHIIGYEHTFTHTIYDF
                     LKSLETRSKAHPDFVDGVRNQAVLDAAMRASAGKRWIRIG"
     gene            5099..5860
                     /locus_tag="VFA54_13700"
     CDS             5099..5860
                     /locus_tag="VFA54_13700"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HZS51916.1"
                     /translation="MVDIHCHLLPGVDDGPADEATALAMCAMAAESGVTDVVATPHAN
                     ATFEFSPQQNEEKRRTLQEAVGDKLRIHLGCDFHLSYENIEAALSSPGRFTINQKQYL
                     LVEFSDQVISQGTSEIFDKMRAAGLTPIITHPERNPLLRNHHARLAAWVRRGCLIQIT
                     ADAVLGRFGRQAESAAISLIDANLAHIVASDGHDLKRRPPVMAECFHFVVDRWGEQKA
                     RTLFEDAPRAVIEGNAITVARPRRRARKKWWVFWR"
     gene            complement(5864..6046)
                     /locus_tag="VFA54_13705"
     CDS             complement(5864..6046)
                     /locus_tag="VFA54_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51917.1"
                     /translation="MESLRRDIDVPEELFAKWMRAGDFEASFRQALQRMYDYRKQVLA
                     IFDSLVANQATLTRPQ"
     gene            complement(6271..7233)
                     /locus_tag="VFA54_13710"
     CDS             complement(6271..7233)
                     /locus_tag="VFA54_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZS51918.1"
                     /translation="MRRRDMLLALAGGAAAAVRPAHAARITRANVCFITDEVSRDLGV
                     ALQFANEFGIRQVELRNVYDKYCFRHEPAKLKEIHAALEQHGVRVAVLSTPILKAVLP
                     GSRLTAEAAREVKAAQRDLPIPDEQQLAMQMDFVRQAIQAAKILETDKLRVFSYWRSE
                     NREELRPQIKDGLSRITEVAEKEKIRLVLENESGCNLADCAETMAVLSQIRSPYLGIN
                     WDVVNGASTGERPYPDGFNLLDKKRVWHMHIKDMRIDLQTGRQQVCAVGDGIIPYAEI
                     FPAMAKAGYAGAVSMETHFSINGQREPASRRSMQGLLKVIDQLD"
     gene            7319..8017
                     /locus_tag="VFA54_13715"
     CDS             7319..8017
                     /locus_tag="VFA54_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51919.1"
                     /translation="MRLFLFAVLEIGCLAVLARAGLVEEARRARDAQDRAGLEAAAAQ
                     AAAAAQAEPQNIRLQYESALIYSYLAEVAMEIRDQAGARKAAEAGIPRAEKAVNGDGR
                     VSEYHRLLGALYGQAIPGNLSAALKYGRKSMDELDRAIALDPKNPEAFLSRGVGKYYI
                     PPMFGGGAQAALEDIRKAIELDPKLDQAYLWEGIVLRKLNRSREAREALNQAVKLNPR
                     QVWAKQQLEKTPPR"
     gene            8049..9806
                     /locus_tag="VFA54_13720"
     CDS             8049..9806
                     /locus_tag="VFA54_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51920.1"
                     /translation="MTKTPPRVCLVLLLFTFLSYFQYPGHTYLVSDTQIYVPMLERFE
                     NPTLFARDPMVIRAHTTWTFFDEIAVGFRKLTRSQLETALGLLHFFARWCLLIGIFMI
                     ARAMGLEDLPAAACAGIYALGGLVQGPSVLLVEYEPVPRAFALGPAVLGIGLAAEHRF
                     AAAGAAGAAALALHGTTSVPFWIILGAFLLAAPSGSITPGNRLWPFLSLGSALLALKI
                     AAPWQLGVSEPQTLLGRLDADWEGLARLRSPYVFVDLWSRAYFWQYGLMFIAAAAAYW
                     RLRKRMSKELRFFAVGLAFLAVLSLPVSYLLLEKWKWALVLQWQPLRSLLFLQMLTIL
                     LALALAMELSCRRADSAGASFWAALAIASAIDSRLLFALTPAAAGCLGRWPIRAAGIA
                     LAVASAWLRPFGITLWTAPLRRELCVVLLLSVLLAGAAALHFRSRLGGLGVVLAVGSA
                     FFIVPGRAQFRWSGEPINRDLAVLSDWARGSTDGNAVFLFPDSPASSIDPGVFRARAE
                     RALYTDWKSGGQINFFRDFARLWGERWRQALAEPFHPEQLVRLRQAGVDYIVLQSAEA
                     GPALPLAFTSGAYRVYRLK"
     gene            9846..10625
                     /locus_tag="VFA54_13725"
     CDS             9846..10625
                     /locus_tag="VFA54_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZS51921.1"
                     /translation="MLPKLTAPDSKQLAEELIGAAGLCRPVPPFSSRYESFTAADAAS
                     VRQRWLELKTASGCKPAGKKIGKVSFGWNGHAQVLRPGWGHILDSTLLVDGTALPCSH
                     LIQPSVEAEFALLLNRDLAGPGITAAHALAAIEGVCAAFEIVDSRFQPKALTPEDAFA
                     DNSSHAYAVIGPHLASPRAIDLAEVNVNLEINDQVRGQGTGAAICGHPVHALAALANL
                     HPLRAGEIILTGSVAGAFPIKSGDRLRATFRRLGVVNLTIE"
     gene            10741..11109
                     /locus_tag="VFA54_13730"
     CDS             10741..11109
                     /locus_tag="VFA54_13730"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZS51922.1"
                     /translation="MRILAIALAALSVAAVIRPAEVPSKTQAKSAPRWKDTELKKAIE
                     ERFARSAIAADKFKVEVNEGVVRITGKTDVIQHKGVATRLAKSMGAKAVRNEIEVSQA
                     ARQKAAAQLAGGAKAKSTQK"
     gene            complement(11146..11331)
                     /locus_tag="VFA54_13735"
                     /pseudo
     CDS             complement(11146..11331)
                     /locus_tag="VFA54_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
     gene            complement(11396..12802)
                     /locus_tag="VFA54_13740"
     CDS             complement(11396..12802)
                     /locus_tag="VFA54_13740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZS51923.1"
                     /translation="MAESAALEKRHVAFRSILGAVVITALKLLTGIATGSLGILSEAA
                     HSGLDLVASGITYVSVRVSDKPADQDHLYGHQKFENFSALLEIGLLLLTSLWIIYEGV
                     RRLLGHPFEVQATPAAFGVMIFSIAVDFLRSRALRRAAAKYQSQALEADALHFSTDIW
                     SSIVVVGGLVAVLLGERYGMRDLQKADAAAALGVAGIILVISVRLGRRSVEALLDAAP
                     AGLAKLLEEALANVKGVATIERVRARHAGNQYFVDANIGIERHAQVEHAKSVSDAARA
                     AIHRVLPGADVMIHTEPRSTGAESLFDIVKAVAARRGLAVHDLTAYDTENGVSLEFHL
                     EVGEKMTLEAAHQMVSELEAEIQREAPQVTQINTHIENEGAEMGRSQVGAEEAETWRG
                     PLAAVARSFPEVLDCHEITVFGSSERPSISCHCVFSGEMPVDRVHEITSQIENRFRQA
                     YPQFQRVTIHPEPHTVLD"
     gene            12951..13895
                     /locus_tag="VFA54_13745"
     CDS             12951..13895
                     /locus_tag="VFA54_13745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZS51924.1"
                     /translation="MAQTPATLPAPGAKPIGPNDVVATVEGEKMTPARIQQLRDSLPA
                     QFQQTASRMDNKAFLKSYGELVALFKLAEKEKLADQEPFKGQLDFLRMNFLAQSYIEH
                     LSRTIDVSQQDFLNYYNEHKADYEEATVRAIYIAFSTTPSAPSSAASGKKPLTEDQAK
                     AKAEALVKQLRNGGDFAKLAQENSDDAATAQKGGELGVIKKSATGVPAELRQVIFGLK
                     AGEVSDPVRQPAGFYIFKAENVRTIPFEEAAVSMAPAVRGAKVRAELDKVLKSVQVSY
                     DNESFFDGSAGVVAPPKPGSSSAPGGSSTSGATTQPKK"
     gene            14017..14586
                     /locus_tag="VFA54_13750"
     CDS             14017..14586
                     /locus_tag="VFA54_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51925.1"
                     /translation="MRRLIFLLVFLVFIPFLAAAQTSGVMPDWEVREAAAALEKHTKV
                     VEGLLNQLKPEDWVAQGAPQLYVEQLKQAKLYNSYLTRQAAMLSAQPDKLSVALDVLF
                     RLDSLHSLLESLTDGTRRHQNPDLADLLASAMSQNSATRERLREYTRELAVEREKEWQ
                     IANEEAQRCRGTLATRPPRPARKPAASKQ"
     gene            14899..16269
                     /locus_tag="VFA54_13755"
     CDS             14899..16269
                     /locus_tag="VFA54_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZS51926.1"
                     /translation="MLKPTIFREYDIRGIADQELDSEGVEQLGRAIGAYIRRERGNRL
                     NVARDCRLSSPRLHEAIVAGLVSSGCEVTDLGMAPTPLLYYASFKLEADGGVMITGSH
                     NPPEYNGFKVMCGKSTLYGEEIQKLREIIASGQFETGSGSVQQEDIVTPYVQEITDQF
                     CFKNWVRFVIDCGNGTAGPVMHQLLEHLRCDKLELFFTPDGRFPYHHPDPTVPKNLES
                     LIDWVKEREAFLGIALDGDGDRIGAVDEKGNIVWGDQLLAIFAREILSRKPGATFISE
                     VKASQVLYDYIRKLGGRAIMWKTGHSLIKAKMQEEKAELAGEMSGHMFFADRYYGFDD
                     ALYAALRLLELVDRANAPLSTLLADLPPTVSTPEIRVDCPDEEKFQVVERVKEELRKN
                     HEIIDVDGVRVLFPDGWGLVRASNTQPVLVLRFEASQPDLLQQYRRQVEDVIAQARSW
                     KGAPAA"
     gene            16270..16818
                     /locus_tag="VFA54_13760"
     CDS             16270..16818
                     /locus_tag="VFA54_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HZS51927.1"
                     /translation="MFVAVGTAREPKVQAVRAALAEIDPRRRWRIETFPVESGVRETP
                     LHTGETRRGARLRVERLIEHFSPHNCRPDLYIGMEGGIASAGGQVWLENWVYASDGQR
                     GYFGSGGALPLPPAIVEEVLHKGRSLADVIDEIAGRNEIRSQEGTWGVLTRNLITRQH
                     VFRDALVNALTPFLLSPSLFRE"
     gene            16909..>19627
                     /locus_tag="VFA54_13765"
     CDS             16909..>19627
                     /locus_tag="VFA54_13765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZS51928.1"
                     /translation="MPSALDLFAPPVRDWFARSFGRPSPAQEKGWPAIAAGRHTLLIA
                     PTGSGKTLAAFLWAIQSLMLHPDGAAEGGARVLYVSPLKALNNDIERNLRAPLEGIVG
                     AGVIRIAVRTGDTPAARRREMVRRPPHILITTPESLYILLTGPQRERIFAGLRFVIID
                     EVHSLASDKRGAHLSLSLERLVELSGEFQRIGLSATVRPVEEVAQFLGGYTAGGSPGG
                     ELRPRPVEIVDAGRRRKLDVKVLSVVEDYATLPDTSIWKEVIPELLELIRSHHTTLVF
                     VQNRGQCERIAAWLNETSRERIAEAYHGSMARPARLQMEARLKAGELRCLVATSALEL
                     GIDIGAIELVVQLQSPKSVTRALQRIGRSGHLVSATSQGRFFPTFRDDLAECAVIARL
                     LDADFLEPIRIPKNAADVLAQQIVACAAVETMDLDRLFALARRSYCYHDLPRPLFDRI
                     ARMLAGRDARELRPAISLDAVNKCVAALSHSRRLAVLNAGAIPDRGLYPAYLAGKKTK
                     VGELDEEFIFETRVGTAFLLGSSVYRLAEVTPHHVVVEPAPGALLPKLPFWHGEGLGR
                     DYELSVAIGRFRRELNLDDPRLLEKLETECRLDRRSAWNLREYFLRQREEAGVLPTDR
                     RIVVETFPDELGNWRMAIHSVFGGRVNGLLELLAVQRAREIMGFQPESMHTDEGVILV
                     FPDGEIPPGNPLKGLAAADAAERAKAEVLGSALFAVRFRHCAQRALLLSRPLPHKRAP
                     LYLSRIRSANLLAQAAAIPEFPLVAETARECLEDALDYENFRRVLEGIESGDIEMVEI
                     ERQTPSPFAAAFQFSFIASFLYDYDAPAAERAAQLLAVNREVLAGVLSPAEAARIIRP
                     EAVESTEAELQFRTQVRRARSAEDLLEILVRVGDLSPAELSAVCASPGLI"
BASE COUNT         3874 a         6032 c         5952 g         3769 t
ORIGIN      
        1 gcccaccgag ttggaggtga ttccctctcc caggacctgc cggcggcgcc cgccaacgcc
       61 cgctaacgcc tcgcttctcg agagccgggg agcagccgcc gcaggcgcag ccatcggact
      121 ccctcgccca ggacagcaat ggccagcacg ccgatgaaga cgtccacggc gtcctccaac
      181 gttcgaaacg ccccaatcgc taccagaatc gtggttgccc cggccggcgg gtggggagcg
      241 cgagcgatca ggctgaaaaa cacggtgagt cccaaagcaa aagtcgccgc ccagacccgc
      301 ggcatggtga gatccttggc tgccagcacc gggggcgcct gcgccgcccc cactgtcagg
      361 acagccagaa atccggcgcc aaggccgatc agatggccca ccaacacgtt gtagaacctc
      421 gaagtgggca acctgggctc cgattccaga taggccgtcg ggcccagact ggcgaagagt
      481 agtggccgcc gagccaaaat cccgatcaaa ccgatgatca tcaccagcgc cccggacgca
      541 accggcaccc aggcccactc aggaacaacg caccagcggc ggtgcaggcg aacgcccata
      601 ggaagcggat ccagccttaa caaaaagggt aagccggaat cgtggcgcag cggactttct
      661 ggcgcacttc cgcttactca gactgcgttc ggactcagcg gttgcgaaga tttcctgccc
      721 cagggaagat ttcctgcccc gggaatgtgt cttttggcgc cggagagagg ttcgggcgaa
      781 agcgcgaaac gccgccgtga ttacccacca caaagccgca cgtcgctacg ttggccacat
      841 gttcaatctc ggctccagga ccaaagccga tacccagcac atgatcctgc ggctttatcc
      901 aagcaagctc aacacccacg ggcttgtcca tccgtgtgtc caagccatta gggcaaccga
      961 tggccgaacc caagagccag cccggacaat cgaagccgcc ctgcgccgcc gcagactcca
     1021 tttcgcgaga cgacaatcgg ccgatgactc attggttcat gaactcacgg cgattcccgc
     1081 ccgaattgat tttcgaaaat ttgatcggga gtttgccctg acgaaccgcc tgcggaccga
     1141 aggaacggac aatctactca cggctgctta cgctgccggg gtgcgccgtt tcatcgcgca
     1201 gagttacgcg ggatggccgt atgcgcgcca aggcggcccg gtaaaaaccg aagaagaccc
     1261 gctcgatccg aatccgccat caggcgtgcg caagacattg gaagcgattc gctacctgga
     1321 atcggcagtt cttggcagcc gcaagatcca aggtgttgta ttgcgctacg ctgcgaggct
     1381 atcggcgcgc gtccgccccg gcacgtcccc cgcttccttg ccaggctcct gatgggggaa
     1441 ctcacagccg tgatgatgac cggcgtccga ggcgcttcca atgcaaaagc caagcgcgaa
     1501 ctgcactggc agcccaggtg gtcaagctgg cgggacggct tccggcatgg attgtctgac
     1561 cggccgattg gccgtgaata tccaggtcaa ttatgccgcg gaatcggcgc ctaggcggac
     1621 atgccgattt cggggaagac gagaagatgt gtcgtgaaat ggggttcgac tcgatccgct
     1681 gccgctgctc acacttaccg gtgggtctat atctttctac atccgcttcc gggaagacgc
     1741 attctcatct cccacggtac gaatccagtg agaaaaggac acagagagtg atcacgggtt
     1801 cgtaccccta catgcgtcat ccaatgtacc cgggttttgt ttcatctcgc tgggcagtcc
     1861 agactgaatc gccgttttag ccgcttaata ggcacgcccg taggagtcgc gacgcgccgg
     1921 ttgctgggtg tcgctcgggc gagggaggcg aaattgggcg ccccgctcag cattcaactc
     1981 gcgggtaaag ggcaggtcca cccggtccag agggattgtc aaattgtttc cgtcccgcgt
     2041 cacatagtgg agcgtcgatc catccagcca ataatcggtc gccgcaatga tggtattgtc
     2101 cttcagcgcg atcagccaat atccctgcgg aacggtctgg acggctcctc ccggcgccgg
     2161 aacggcctgc tgccccacta cattcggata aggttcggaa taatcgtagg gtgggtagta
     2221 atactgtgga tagtagtagt agggataacc gtagccgtac ggccaccaat agtcgtaagc
     2281 aaaaggccag taggcgaagc tgtagggata aaagcccagg ccgacgctaa agaagacgcg
     2341 gctgcggcgg aagccgaatc tggagaatcc cgggtggaat ccaccccgaa atacgccgct
     2401 tcggacgaaa cctccgcgga acgccccgct gcggacaaag ccgccgtgga acgagcctcc
     2461 gccgatgaag ctgcctccag cgaatccgcc gccgatgcgc ccgcccgaaa agccgccgcg
     2521 catgcctccg aacccgccgt gaaagccacc acctccgtga aaaccaccgc ctccatggaa
     2581 gcccccccgt tgcgccggca gggatatcgg aaaaaacagc aataaggagg ccgcaagcaa
     2641 ggcgagcgcc cccgggcgcc gcccattgcg agagttcgcg tgaaacatgg ccgaacctct
     2701 tctttcatta tacggctcgc ggcgcagcct ccccaaactc ttttccccgg gccggagagg
     2761 cgggggtctg gctccgggga ccgcgcaagg ccgcgcctgg agttaacaca tggacagccc
     2821 gcaattcctg cttcgcacgc cagttcgtag cccgttcggt ctttgtgcta tagtgaggcc
     2881 gaaaggggga gaagaatgga tgacaagagg ctgtcttact tcctgttcgg attaggaatg
     2941 ggagtcggca ttggaatcct gtttgctccc aagtcgggtg aggaaacccg cgagatcctg
     3001 aagggcaaag ccggcgaggg gcgtgaattc ttgcggcgca agacggaaga gattcgcgac
     3061 tcgactaccg agatactgaa caagggccgc gaagcttttc aatcgcagaa agagaacctg
     3121 gcggcagccc tggaagcggg caaacaggcg tatcgtcaaa gcgtctcggc cgaccagccc
     3181 cggagttcgg aaggcccggc cggaatgtaa aagatccgcg gtcggcagct tagcccggcc
     3241 cagtgacgaa ctaacccatg gacacgcgct ttctgctgat tatcagcatc ttcacgggag
     3301 tagcggcagt tgctttgctg gttcagatgt tcgtgcttgt gggactctac ctcaccgcca
     3361 aggcgacgca ggcgcgcatc actagtttgg ccgaccgcgt agaaccccta attgatacca
     3421 gccgccgcat ggccgaagaa acccggcagc aagccagaga aatactgggg aagctgactc
     3481 agattacaga agccacccgc gttcaggtgc ttcgcatcga tgaaatgctg gcggaagtca
     3541 gcgtccacac gcgttcgaat ctggagctgg tcgaccgggc tgtggccgcc acagccgcca
     3601 aaatcgagga aacggtcaac cggtggcagc ggaccatcct cgctccggtg cgcggcatca
     3661 atggagccgc cgccgccgtt cgcgccgttg tgtcccacct gggaaggcgc cggtcggcag
     3721 acgggcgtcc cactcaggaa gaagatctct tcatttagcg cttcgccttc agcaccaaag
     3781 cattctacat ctctttatgt cttacaagtg tatacgcact ggtgtagtcg gggatttcac
     3841 gccccgaggc ttgctttccc ccccaaggcg acgaaccaaa atgaacgatc ggatctgctg
     3901 ctccgggggg ccgtttctag taccattgaa agtctttttg caggattata tctctgggtg
     3961 aggtgaaatg ccgatacgag tcgctctgct gggaaccaag ttcatgggca aagcccacag
     4021 ccaggcctat cgcagcgtca acatgtactt tcccgatgcg ccgcaggtcg agatgaaggt
     4081 gattgtgggc cgaaacgcgc aagaaacaga gactgcccgc gctctttacg gatggcagga
     4141 ggcttcgacc gactggcaaa aaacagtggc ccgcgccgat gtggatctgg tggacgtgtc
     4201 cacgcctggg aacctgcatt gtgaaatggt ccttgccgca gccaaggcgg gcaagcacgt
     4261 ggtgtgcgag aagccgctgg ccaataccct cgcggaagcc gaacggatgc tggaagccgt
     4321 ggagaaagcc aaggtccggc atttgctgat gcacaactac cgccgagcgc cggccgtggc
     4381 tatggcaaaa aagcttgtgg aggaagggcg cctaggcaag ctctatcatt tccgggcccg
     4441 ctacttgcag gattgggcga tgtcgccgga tcttcccatg ctgtggcgtt ttgtagccca
     4501 atccgcaggc tcgggcgcgc tgggcgacct gggagcccac attgtggacc tggcgcgcta
     4561 tctctgcggc gaaatcaccg aggttgccgc cgccatggag acattcatca aacaacggcc
     4621 ggtcgctccc ggctcatccc gcaaggcgaa ggtcacggtc gatgacgccg ccgccatggt
     4681 ggtgcgattt aagaatggcg cgctggggac tctagaagcc agccgattcg ctcccggccg
     4741 taagaatcag aacacatttg agattaacgg aagcaaagga tcggtggaat tcgatctgga
     4801 agacctgaac gtcctgaaat ttttctccac cgaagacccg cccgacgttc aaggctttca
     4861 taaagtgcta tgcctcgcga aaggcgtgca cccttatgcc agcgcgtggt gggccgacgg
     4921 gcacattatc ggctacgagc acacgttcac ccacacgatt tacgattttc tgaaatcgct
     4981 ggagacgcgg tccaaagctc atcccgattt tgtggacgga gtccgcaacc aggcggtgct
     5041 cgacgcagca atgcgggctt cggccgggaa acgctggatc aggatcggct gatccgctat
     5101 ggtcgatatt cactgccatc tgctgccagg ggtcgacgac ggacccgccg acgaggctac
     5161 ggcgctggct atgtgcgcaa tggccgccga aagcggcgtg acagacgtcg ttgccactcc
     5221 ccacgccaat gcaactttcg aattttcccc gcagcaaaac gaagagaagc gccggacgtt
     5281 gcaggaggcc gtgggagaca agttgcgcat ccacctgggc tgcgattttc atctcagcta
     5341 cgaaaatatc gaagcggccc tttcttctcc cggacgcttc acaatcaatc agaaacaata
     5401 cctgctggtc gagttttccg atcaggtgat ttcacaaggc acgtcggaga tttttgacaa
     5461 aatgcgggcg gccggcctga cgccgatcat cacgcatccg gagcgcaacc cgctgctgcg
     5521 aaatcaccac gcgcggcttg ccgcctgggt gcgccgcggc tgcctgattc agataacggc
     5581 cgatgccgtg ctgggccggt tcggccgcca ggccgaatcc gccgcgattt ccctgatcga
     5641 cgccaacctg gcccacatcg tcgcttccga cggccacgac ctgaaaaggc ggccgccggt
     5701 gatggccgag tgctttcact tcgtcgtgga ccgctggggt gaacaaaagg cgcgcacctt
     5761 gtttgaggat gccccgcggg ccgtgatcga ggggaatgcc attactgtgg cccggccgag
     5821 aagacgcgcg cgcaaaaaat ggtgggtttt ctggcggtag cggctactgc gggcgggtga
     5881 gagtggcctg attggcgacc agcgaatcaa agatcgccag cacctgtttg cgatagtcat
     5941 acatccgttg gagggcttgc cggaaagaag cctcgaaatc gcccgcacgc atccatttgg
     6001 caaacagctc ctctggcaca tcgatatccc ggcgtaggga ctccactcgg tgacctcgca
     6061 aaaacagagc gtagaaaaag gccgcctcac ggtgcggaga ctgctctggt gatttgaacg
     6121 ccgttgacat agccggtccc acggatcact gcctcggaat ttacgatccg taagaaatgc
     6181 cagtataatc agaccggctt ttggccgcaa gtacaaactg cccggagccg actctaggga
     6241 attccccagt cggcggaacc cgatgccggg ctagtcgagc tgatcgatta ccttcagcag
     6301 gccttgcatg gaacgccggc tggccggttc cctttgcccg ttgattgaga agtgagtctc
     6361 cattgacact gcgccggcat agccggcttt ggccatggcg ggaaagatct cggcgtatgg
     6421 aatgatgccg tcacccaccg cgcagacctg ctgccgcccg gtctgcaggt cgatgcgcat
     6481 atctttgata tgcatgtgcc acacgcgctt cttatcgagc aggttgaatc catccggata
     6541 cggcctctct ccggttgagg cgccgttgac aacgtcccaa ttgatgccga ggtaaggcga
     6601 gcggatctgg ctcagcaccg ccatggtttc ggcgcaatcc gccagattgc agccgctctc
     6661 gttttcgaga accaggcgga ttttctcttt ctccgccact tcggtgatgc ggctgagccc
     6721 atccttgatt tgcgggcgca attcttcccg gttttccgat cgccaatacg agaagacgcg
     6781 gagcttgtcg gtctccagaa tcttcgccgc ctggatggcc tggcgcacga aatccatctg
     6841 catagccaac tgttgctcat ccggaatcgg caagtcgcgc tgggcggcct tgacctctcg
     6901 cgcagcctcg gctgtgagcc ggctgccggg taaaacggcc ttcaaaatcg gggtcgaaag
     6961 aacggccact cgcacgccgt gttgttccag cgcagcgtga atttccttga gcttcgccgg
     7021 ttcgtggcga aaacaatact tatcataaac gttgcgcagc tcgacctggc gtatcccgaa
     7081 ctcgttggcg aactgaagcg ccacgcccaa atcccggctg acctcatcgg ttatgaagca
     7141 aacattcgcc cgcgtgatcc gtgccgcatg cgccgggcga actgcggccg ccgcgccgcc
     7201 ggccagagcc agcaacatgt ctcttctgcg catgatctcc tcccagcgat gcgtagtccg
     7261 cagacgggac gcctgcccca gcgctatctt acaataatgg ctgccggaaa attccgttat
     7321 gcggttgttt ttgttcgctg ttcttgagat cggctgcctg gcagttctgg cacgcgctgg
     7381 tcttgtggag gaagctcgcc gggcgcggga tgcccaggat agagcgggcc tggaagcggc
     7441 cgccgcccag gccgcagccg ccgctcaggc ggagccccaa aacatccggc tacagtacga
     7501 atccgcgctc atttattcct atctcgccga agtggcgatg gaaatccgcg accaagccgg
     7561 cgcccgcaaa gctgccgaag caggtattcc acgcgccgaa aaagccgtta acggcgacgg
     7621 ccgcgtgtcg gaataccatc gcctgctcgg cgctctttac ggccaggcca tccctggaaa
     7681 tctgagcgcc gcgctgaagt atgggcggaa gagcatggat gagctcgatc gggcgattgc
     7741 tctggacccc aagaatcctg aagcctttct cagccgcgga gtcggcaaat attacattcc
     7801 tcccatgttc ggcggcggcg cgcaggccgc tttggaagac atccgcaaag caatcgaact
     7861 tgatccgaaa ttggatcagg cctatctttg ggagggtatc gtacttcgca agctgaaccg
     7921 aagccgcgag gcgcgggaag ccctgaatca agctgtgaag ctgaatccac gccaggtttg
     7981 ggccaagcag cagctcgaga agacgccgcc ccggtaatgc cgcaagagcc agcgagcagg
     8041 acacagcctt gaccaaaacg cccccccgcg tctgtctggt ccttcttttg tttacttttc
     8101 tttcatactt ccagtatccc ggccacacct acctggtgag cgatacccaa atctacgttc
     8161 cgatgctcga gcgtttcgag aacccgacgc tcttcgcgcg ggacccgatg gttatccggg
     8221 cccacactac ctggaccttc ttcgacgaaa tcgctgtcgg tttccgcaag ctgacgcggt
     8281 cgcaactcga aaccgcgctg ggcttgcttc atttttttgc ccgctggtgc ctgctcatcg
     8341 gaattttcat gatcgcccgc gcgatggggc tggaagatct gccggctgcg gcgtgtgctg
     8401 gaatctatgc tctgggcggc ctcgttcagg gtccttccgt gctgctggtc gagtacgagc
     8461 cggttcctcg cgcctttgcc cttgggccgg ccgtcctcgg aattggcttg gcggcggaac
     8521 accgcttcgc cgcagccggc gcggcgggcg ccgccgctct ggcattgcac ggcacgactt
     8581 cggttccctt ctggatcatt ttgggcgctt ttcttctggc cgcccccagc ggctcgataa
     8641 cgccaggcaa ccgcctctgg ccgtttctgt ctctgggatc cgcccttctg gctctgaaaa
     8701 ttgcggcccc atggcagctc ggagtcagcg agccgcaaac cctgctgggc cgcctggatg
     8761 cggattggga aggcctggcg cggctgcggt ctccctatgt attcgtcgat ctgtggtctc
     8821 gcgcttattt ctggcaatat ggcctgatgt tcattgccgc cgcggccgcc tattggcggc
     8881 ttcggaagcg aatgagtaag gagctgcggt ttttcgctgt aggactcgcc ttcctggctg
     8941 tgctgagcct gcctgtttcc tacttgttgc tcgagaaatg gaaatgggcg ctggttcttc
     9001 aatggcagcc tctgcgctct ctcctcttcc tacagatgct gacaattctg cttgcgctgg
     9061 ctctcgccat ggaattgagt tgccgcagag cagacagcgc gggggccagt ttctgggccg
     9121 ccctggcaat cgcctcggca atcgactcgc ggctgctgtt tgcgttgacg ccggcggcgg
     9181 ccggctgcct ggggcgatgg ccgattcgag cggcagggat tgctctggcg gtggcgtcag
     9241 cttggttgcg gcctttcggg atcactcttt ggaccgcgcc gttgcgacgt gaactttgcg
     9301 tagtgttgct tctgtcggtt ttgctggcgg gcgcggcggc actccatttc cgctcgcgcc
     9361 tcggcggtct tggggtcgtc ctggcggttg gatccgcatt ttttattgtg cccggcaggg
     9421 cgcagttccg ctggtcgggc gaacccataa atagggattt ggccgtcttg agcgattggg
     9481 cgcgcggatc cactgatggg aacgccgttt ttcttttccc tgatagtccg gcttcttcga
     9541 tagatcccgg ggtttttcgc gctcgcgcgg aacgggccct ttacaccgac tggaagagtg
     9601 gaggacaaat caatttcttt cgcgatttcg cccgcctctg gggtgaacgc tggaggcaag
     9661 ctctcgcgga acccttccac ccggaacaac tcgtccgcct ccggcaagct ggcgtggatt
     9721 atatcgttct gcaatccgca gaagccggtc cggccctgcc gctggccttc accagcggcg
     9781 cctatcgcgt ctatcgcctg aaatgagccc cgcgcaaagt gcgtttcatg ccaaaatgtt
     9841 gccttatgct accgaagctg acggctcccg attcaaaaca actagccgag gaactcattg
     9901 gcgcagccgg cctgtgccgg ccggtccctc ctttcagctc acgctatgaa tcgttcaccg
     9961 cggccgacgc cgcgtccgtg cgccagaggt ggctggagct caagacagct tctgggtgca
    10021 agccggctgg aaagaaaatc gggaaagtaa gcttcggctg gaacgggcat gctcaagtgc
    10081 tgcgcccggg ctggggacac attctcgaca gtacgctcct ggtggacggc accgcgctcc
    10141 cctgctccca cctgatccag ccgtcggtcg aagccgagtt tgcgctgctg ctcaatcgcg
    10201 atctcgccgg ccccggcatc accgctgcgc acgctctggc cgcgattgaa ggcgtgtgcg
    10261 cagcgttcga aatcgtggac agccggttcc agcccaaggc gctcactccg gaggacgcgt
    10321 ttgccgacaa tagctcccac gcctatgccg tgatcggtcc gcatctggca tctccgcgcg
    10381 cgatcgactt ggccgaagtg aacgtcaacc tggagatcaa cgaccaggtt cgcggccagg
    10441 gcactggggc ggcgatctgc ggccatccgg ttcacgctct ggctgctctg gccaatcttc
    10501 acccgctccg cgccggcgag atcattctca ccggatcggt agccggcgca tttcccatca
    10561 agtccggcga ccggcttcgc gccacattcc ggcggctcgg agtggtgaac ctgacgatcg
    10621 agtagcgcgc agcagcgttc cgatttaaac tattttggac gtcgcagacg ctggctctgg
    10681 acgctgcgcc accgttctct ggccgcagtc cccaaaattt agtcccaaga aggagcaccc
    10741 atgcgcattc tcgcgatcgc actggcagcc ctgtccgtgg ccgcggtgat tcggcccgcg
    10801 gaggttccga gcaaaacgca agccaaatcg gcgccgcgct ggaaagatac ggaactcaag
    10861 aaagcaatcg aagagcggtt cgcccgttcg gcgatcgccg cggacaagtt taaggtcgag
    10921 gtaaacgaag gcgtcgtgcg catcaccggc aaaaccgacg tcatccagca caagggcgtg
    10981 gcgacgcgcc tggcaaaatc gatgggcgca aaggccgtgc ggaatgaaat cgaggtaagc
    11041 caagcggcgc gtcagaaggc tgcggcccag ttggccggcg gcgcgaaagc caaatccacg
    11101 caaaagtaaa cgagcgccag gcgaagtcgt ccggcccgat cgcgatcagg cgggcagcag
    11161 tttgtccagc tcttcccggt cgaagccgat aatcacccgg ccgtcaatca cgatagtggg
    11221 cgtgatacgg ctgcgatgca cttcgatcag gtcttccagc gcgtcagaat cccgggttac
    11281 gtcgaattcc tggaatgcaa caccttttgc caagagatac tctctcagtg agcggcaggg
    11341 cggtcaggtg ggctgcgagt ataacacaat acgcattatg tttattatcc cacgcctagt
    11401 ctaataccgt gtgcggctca ggatgaatcg taacgcgctg aaactgggga taggcctgcc
    11461 ggaagcgatt ctcgatttgc gaggtgattt catggacccg gtccacgggc atctcgccgg
    11521 agaagacaca atggcaggag atggagggcc gctcgctcga gccgaaaacc gtgatttcgt
    11581 ggcaatcgag cacctcggga aaactgcggg ccacggcggc cagcggcccg cgccacgtct
    11641 cggcttcctc ggcgcccact tggcttctgc ccatttctgc tccctcgttt tcaatatgcg
    11701 tgttgatctg agtgacctgg ggcgcttcgc gctgaatttc ggcctccagc tcagagacca
    11761 tctgatgagc cgcttcgagc gtcatttttt cgccgacttc gagatgaaat tctaaggaga
    11821 cgccattctc tgtgtcgtag gcggtgagat catgaaccgc caggccccgg cgggcagcga
    11881 ctgcctttac gatatcgaac agactttctg caccggtcga acgaggctct gtgtgtatca
    11941 tgacgtcggc gccgggaagc acgcggtgga tggcggcgcg ggcggcgtcc gagacggatt
    12001 tcgcgtgttc cacctgcgcg tgccgctcga tgccgatgtt ggcgtcaacg aaatactggt
    12061 ttcccgcgtg acgcgcacgc acccgctcga tggtggccac ccctttgaca ttcgccagcg
    12121 cttcctccaa gagtttggcg agccccgcgg gggctgcatc aagcagcgcc tctacagagc
    12181 gccttcccag gcgcacactg ataaccagaa tgatccccgc cacgcccaga gccgccgccg
    12241 catccgcttt ttgaagatcg cgcatcccat agcgctcccc gagcaaaacg gcaaccagtc
    12301 ccccaacgac aacgatcgac gaccaaatgt cggtggagaa atgaagagca tcggcctcga
    12361 gggcctggct ctgatacttc gcggccgccc tgcgcagcgc gcgagagcgc aagaaatcga
    12421 cggcgatcga aaaaatcata accccgaacg ccgccggcgt ggcttggacc tcgaagggat
    12481 gtccgagcaa tctgcgcacg ccttcgtaaa tgatccacag agaggtcagc agcagcagcc
    12541 cgatttcgag cagagcggaa aaattttcga acttctgatg gccgtaaagg tgatcctggt
    12601 ccgcgggctt atcggaaaca cgcacggaaa cgtaggtgat ccccgaagcg acaaggtcca
    12661 agcccgagtg cgcggcttcc gacaggattc ccaggctgcc ggtagcgatc ccggtcaaca
    12721 atttcaaggc agtgattacg acagcgccaa gaatcgagcg aaaagccaca tggcgcttct
    12781 caagggcggc cgactcagcc atcttttacg agaataccag aggcggcggg ttctccacgc
    12841 cctgtgatag aatcgcaaga gtcaaagcgc ctccggaatg gtggacctag atgcttgaat
    12901 caacaatttg ccgctttgcc ggcgcactca ctttagcaag cgttctgtca atggctcaaa
    12961 ccccagctac gctgcccgcc ccaggcgcga agccgatcgg gcccaacgac gtggtcgcca
    13021 ccgtggaggg cgaaaaaatg acgcctgccc ggatccagca actgcgcgac agcctgccgg
    13081 cgcagttcca gcagaccgcc agccgcatgg acaacaaagc cttcttgaaa agctacggcg
    13141 aactggtggc tctcttcaaa ctggctgaga aggagaagct ggctgaccag gaacccttca
    13201 agggccagct cgatttcctc cggatgaact ttctggccca gtcctacatc gagcatctaa
    13261 gcagaaccat cgatgtcagc cagcaggatt ttctcaacta ctataacgag cacaaggcgg
    13321 actacgaaga agcaaccgtc cgcgccattt acatcgcgtt ctccacaaca cccagcgccc
    13381 cttcaagtgc ggcaagcggc aaaaagccat tgaccgaaga tcaggccaaa gccaaagccg
    13441 aagcgctcgt gaagcaattg agaaacggcg gcgacttcgc caagctcgct caggaaaatt
    13501 cggatgacgc agccacggcc caaaaagggg gcgaactggg cgttatcaaa aagagcgcta
    13561 cgggcgtgcc ggccgaactc cggcaggtga ttttcggtct caaggcggga gaggtaagcg
    13621 atccggtgcg gcagcctgcc gggttctata ttttcaaggc ggagaacgtg cggaccatcc
    13681 cgtttgagga agcggctgtc agcatggccc ctgccgtgcg cggcgcgaaa gtgcgggcgg
    13741 aactcgacaa ggtgctgaag tctgtccagg tcagctacga caacgagagc ttcttcgacg
    13801 gatccgccgg ggtcgtcgca cctccgaaac cgggatcctc ctctgcgccg ggcggctctt
    13861 ctacgtctgg tgcaacaacg cagcctaaga aataagagcg ggtaacttca gagcggttac
    13921 ggccccggac accttcgagc ccagcccgct gcgcataagg catcgctgtc gtttgcctgg
    13981 ttgcctggcg ggcccagtta ccctactatg gcaagtatga ggcggctcat cttccttttg
    14041 gtcttcttgg ttttcattcc attcctcgcg gcggcccaaa cgtccggcgt gatgcccgat
    14101 tgggaggtcc gcgaggcggc cgcagctctc gagaagcaca cgaaggtcgt cgagggtctt
    14161 ctcaatcagt tgaagcccga ggattgggtg gcgcagggcg cgccccagct ttatgtggag
    14221 cagttgaaac aggccaagct ctacaattcc tacctcacgc ggcaggccgc aatgctgtcg
    14281 gcgcagcccg acaagctgag cgttgccttg gatgttctgt ttcgtttgga ttctctgcat
    14341 tccctgctcg aatctctcac cgacggaacc cgccggcatc aaaatcccga cctggcggat
    14401 ctgctggcct cggcgatgag ccaaaattca gcaacacgag agcggctgcg cgagtatacg
    14461 cgcgaactgg ctgtggagcg cgagaaagaa tggcagatcg ccaatgaaga agcccagcgc
    14521 tgccggggaa ccctggccac gcgcccgccg cgcccggcca ggaagccggc ggcttctaaa
    14581 cagtaagccc cgaaacttcc gccgcacatg ctccgcattg ccccaaatcc cggacgagcg
    14641 cctctgcgcg ggatccgaaa agcgctgcga ggaatcagca gcatagcggc tgtgccttgt
    14701 ggaaccgccg gaaggatgct atcaacgtaa tgtatggcgt ggcaatgtca catctgtgcc
    14761 gaagcttcgg aggatatctg tcctgcttgc accaaggacg tctgcaaaaa ccacatctgt
    14821 gaggtttgcc ggcgttgcag cgattgctgc tgctgtgaag aggagccggt ccatcgcgca
    14881 atcaccggag gatagcggat gctgaagcct acgatttttc gtgaatacga cattcgaggc
    14941 atcgcggatc aggagcttga cagcgaaggc gtcgagcaac tggggcgcgc catcggcgcc
    15001 tatatccgcc gcgaaagagg caatcggctg aatgtcgccc gggattgccg gctcagctct
    15061 ccgcgcctgc acgaagccat cgtcgcgggg ctggtctcca gcggctgcga agtgaccgat
    15121 ctgggcatgg cgcctacgcc gttgctgtat tacgcctcgt tcaagctgga agcggatggc
    15181 ggcgtgatga tcaccggcag ccacaatcct cccgagtaca acggcttcaa agttatgtgc
    15241 ggcaaatcga cgctgtacgg cgaagagatt caaaagctgc gcgagatcat cgcctctggg
    15301 caattcgaaa ccggctcggg cagcgtgcag caggaagaca tcgttactcc atatgtgcag
    15361 gagattaccg accagttctg ctttaagaat tgggtccgtt tcgtgattga ttgcggcaat
    15421 ggaaccgcag gtccggtcat gcaccaactg ctcgagcatc tccggtgcga taagctcgag
    15481 ctgttcttca cccccgacgg ccgcttcccc taccatcatc cggatcctac cgtcccgaag
    15541 aatctggaat cgctgatcga ttgggtgaaa gaacgggagg cttttctggg catcgccctg
    15601 gacggcgacg gcgaccgcat cggcgccgtc gatgaaaagg gcaacattgt gtggggcgac
    15661 cagttgctcg ccatcttcgc ccgcgaaatt ctatcccgca agccgggtgc gacgttcatt
    15721 tccgaagtga aggcgtcgca ggtcctttat gactacattc ggaagcttgg cggccgcgcc
    15781 atcatgtgga aaacgggcca ctccctcatc aaagccaaga tgcaggaaga gaaggcggag
    15841 ctcgccggcg agatgagcgg ccacatgttt ttcgccgacc gctattatgg cttcgatgac
    15901 gccctctacg ccgcgctccg gctgctcgag ttggtggacc gcgccaatgc tccgctttcc
    15961 acgctgctgg cggatttgcc gcccacggta agcactccgg agatccgtgt ggattgcccg
    16021 gatgaagaga aatttcaggt ggtcgagcgg gtcaaagagg aacttcggaa aaatcacgag
    16081 attatcgatg tggacggagt tcgcgtgttg ttcccggatg gatgggggct ggtgcgcgcc
    16141 tcgaatacgc agcctgttct ggtgctccgt tttgaggcct cgcagccgga cctgctccag
    16201 cagtaccggc gtcaggtgga agacgtgatc gctcaggcca gaagctggaa gggagctccg
    16261 gccgcctgaa tgtttgtcgc ggtcggcacg gcccgggagc ccaaggtgca ggccgttcgc
    16321 gccgcactcg ctgaaatcga tccgcgccgg cggtggcgga ttgagacctt cccagtcgaa
    16381 agcggcgtgc gggaaactcc tcttcacacc ggcgagaccc ggcggggcgc gcgcctgcgg
    16441 gtggaacgcc tgatcgaaca tttcagcccg cacaattgcc ggcccgacct ctacatcggc
    16501 atggaagggg gcatcgcctc cgccggcggc caagtctggc tggaaaactg ggtatacgcc
    16561 agcgacggcc agcgcgggta tttcggcagc ggcggcgcgc tgccgctgcc cccggccatt
    16621 gtggaggaag ttctccacaa gggccggagc ctcgccgacg tgatcgacga aatcgccggc
    16681 cggaacgaga ttcgcagcca ggaaggcaca tggggcgtcc tgacacgaaa cctgattacg
    16741 cgccagcacg tgtttcgcga cgccctggtg aacgccctta ctccctttct tctgtcgccg
    16801 tctctgtttc gcgaataaac gcccgggggg ccgaactccg ccgggtaagc agggcttctc
    16861 cttcgctatg atggtggatc acggcttgct tgcagttcgc ttccctatat gccgtccgct
    16921 ctcgatctgt tcgctccccc tgttcgcgac tggtttgcgc ggtcgttcgg tcgcccttct
    16981 cctgcgcagg aaaagggctg gccggccatc gcggcgggcc ggcatacgct tctgatcgcg
    17041 cccaccggct ccggcaaaac actcgccgcg ttcttgtggg cgattcaatc gctgatgctg
    17101 catccggatg gcgccgcgga aggcggcgcg agagtgctgt acgtctctcc gctgaaggcg
    17161 ctcaacaatg atatcgagcg caacctgcgg gcgccgctgg aaggcattgt gggcgccggc
    17221 gtcattcgca tagcggtgcg caccggcgac acccctgccg cccggcgccg cgagatggtc
    17281 cggcggccgc cgcatattct gatcactacg ccggaatcgc tctacattct gctgaccggg
    17341 ccgcagcgcg agcggatctt tgccggcctg cgctttgtga ttatcgatga agtgcattcg
    17401 ctggccagcg acaaacgggg cgcgcatctt tcgctgtcgc tggaacggct ggtcgagctg
    17461 agcggcgaat tccagcgcat cggcctttcc gccacggtgc gcccggtcga agaggtggcg
    17521 caatttctcg gaggatacac ggccggcggg tcgcccgggg gagagctgcg gccccggccg
    17581 gtggaaattg tggacgctgg aaggcggcga aagctcgatg tcaaggtcct ctcggttgtg
    17641 gaggattacg caacccttcc ggatacgagc atctggaaag aggtcatacc cgaactgctg
    17701 gagctgattc gctcccacca cacaacgctc gtcttcgtgc agaaccgggg ccagtgcgag
    17761 cgaatcgccg cctggctgaa cgaaacttcc cgggagcgta tcgccgaagc ctaccatggc
    17821 agcatggcgc gtccggcgcg gcttcaaatg gaagcgcggt tgaaggccgg cgagctgcgg
    17881 tgtctggttg ccacatcggc tctggagttg ggcatcgaca ttggcgccat cgagctggtg
    17941 gttcaattgc agtcgccgaa atcggtgacg cgcgctttgc agcgcatcgg gcgttccggc
    18001 catctggtga gcgcgaccag ccagggccgc tttttcccca cctttcgcga cgacctggcc
    18061 gagtgcgcgg tgatcgcccg tttgctggac gccgatttcc tggaaccaat ccgcattccc
    18121 aagaacgccg ccgatgtgct cgcccagcag attgtcgctt gcgccgcagt ggagactatg
    18181 gatctcgacc ggctcttcgc tctggcccgg cggtcttatt gctaccacga tctgccgcgc
    18241 ccgctctttg accgcatcgc aagaatgctg gcgggcaggg atgcgcgcga gctgcgtccg
    18301 gcgatttctc tggatgcagt gaataaatgc gtcgcggcgc tttctcacag ccgccgcctg
    18361 gccgtgctta atgccggcgc tatccccgac cggggccttt acccggctta tctggccggt
    18421 aaaaaaacca aagtcggaga gctcgatgag gagtttattt tcgagacccg cgtggggaca
    18481 gccttcctgc tcgggtcgag cgtttaccgg ctggccgagg tcacgccgca ccacgtagtc
    18541 gtagagccgg ctcccggcgc cctgctgccc aagctgccct tctggcacgg cgagggcctg
    18601 gggcgcgatt acgagctgag cgttgccatc ggccgcttcc ggcgggagtt gaacctggac
    18661 gatccccggc ttttggagaa actcgaaacg gaatgccggc tcgaccgccg cagcgcctgg
    18721 aacctccggg aatattttct gcgccagcgc gaagaagcgg gagtgctgcc tacggaccgc
    18781 cgcatcgtgg tcgaaacgtt tcctgacgag ctggggaact ggcgcatggc gatccactcg
    18841 gtatttggcg ggcgggtgaa tggcctgctg gagttgctcg cggtccagcg ggctcgggaa
    18901 ataatgggat ttcagccgga gtcgatgcat accgatgagg gcgtcattct ggtttttccc
    18961 gacggcgaga tcccgcctgg caatccgctc aagggtctcg ccgccgccga tgccgccgaa
    19021 agagccaagg cggaggtcct cggctccgct ctcttcgctg tccgtttccg tcactgcgcc
    19081 cagcgggcgc tgctgctctc ccggccgctg ccgcacaagc gcgctcctct gtacttgagc
    19141 cgcatccggt cggcgaacct gctggcccag gcagcggcga ttcccgaatt tcccctggtg
    19201 gccgaaaccg cccgggagtg tctggaagac gctctggatt acgaaaattt tcgccgagtc
    19261 ctggaaggga tcgagagcgg cgacatcgag atggtcgaaa tcgagcgcca aaccccttct
    19321 ccttttgcgg cagcgtttca attcagcttc attgcgagct tcctgtatga ctacgatgcg
    19381 ccggcggcgg agcgcgccgc gcaactgctc gccgtcaatc gcgaggtgct cgctggcgtg
    19441 ctttccccgg ccgaggcggc ccgcatcatc cggcctgaag ccgtcgaatc caccgaagcg
    19501 gaattgcagt tccggaccca ggtgcggcgc gcgcgatccg ccgaggatct gctggaaatc
    19561 ttggtgcgtg tgggagactt gagccccgct gaattgtccg cggtatgtgc ctcgccgggc
    19621 ctgattc
//
LOCUS       DASREK010000255         8941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_616011, whole genome shotgun sequence.
ACCESSION   DASREK010000255 DASREK010000000
VERSION     DASREK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616011"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFA54_13770"
     CDS             complement(<1..395)
                     /locus_tag="VFA54_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZS51929.1"
                     /translation="MRQSSGKFKIALLQMRCSPDTGANLARAIGRVEEAAQNGAQVIC
                     LPELFRSQYFCQREDPALFDLAEPIPGPTTEALSAAARERRVAVVAPLFERRAAGVYY
                     NSVAVIDADGSIVNVYRKTHIPDDPLYYE"
     gene            455..2080
                     /locus_tag="VFA54_13775"
     CDS             455..2080
                     /locus_tag="VFA54_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZS51930.1"
                     /translation="MLSYTRGPEIPLLDKTIDQAFRDSAARFPDREALVVPHQRARLV
                     YRELEAQVERTARGLAGLGLRPGDRAGVWATNCVEWVLLQLACARAGLVLVNVNPAYR
                     SRELGYVLRKSGMRALFLHEKDPRADYRTILDQALSEAAGERPALEQIVYLGQESWER
                     MLARPGQLPADPAAPDDVTNIQYTSGTTGAPKGVLLTHRNLVNNAWVVAQAMRFTAQD
                     RLCSPVPLYHCFGCVMSSLLAVVAGGTLILPSAQFDPLATLWAIHSERPTALYGVPTM
                     FIAELEHPEFLRFDLRSLRTGIMAGAPCPVEVMKRVVGEMHCPEMTIAYGQTEASPAV
                     TMSKVDDPLELRVSTIGCALPNTEVKIVAPGSGETVPVGEQGELCTRGYLVMKGYDQD
                     PDATQRAIDKENWLHTGDLAVMQPNGYFRITGRARDLIIRGGENIYPREIEEFLYTHP
                     KIADVYVVGLPDLKLGEAVLAWIKLKAGTSATEEEIREFCRGKIAHFKIPQYIRFVDS
                     FPMTVTGKVQKYRIREEEIRARGLEEAAKVETA"
     gene            complement(2162..3424)
                     /locus_tag="VFA54_13780"
     CDS             complement(2162..3424)
                     /locus_tag="VFA54_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HZS51931.1"
                     /translation="MRKLFVFVLVGMIPLAAHGQTHPLQALIEAARANSPSLKDLLPK
                     TLFDLKERGASAVWGQDFLFAVETDKTASVSLDGNAPETMTRVPGSNLWYLVKKMRIG
                     VTHNYQFYADGQSMGQAYRYDVAGYGPDSYPQPGVPKGALSEKKTITSKIYPDWNPEY
                     WVYVSPGYDPSRGAALMVWQDGQNLARGDLYAMRLFTVTENLVHQKLIPPMIHVLVAP
                     KDGTRHRSIEYDTVSDRYGRFLLEEILPEVEKTYKIRSDGYSRGIGGQSSGGICSLNV
                     AWYFPDKFSRVLSNIGSYTALQWRPEEHLDGGNIYPFKIRREPKRNIRVWLADGSEDQ
                     ESPRAGSWPLQNIQLANSLKLRGYDFHFRFGDGMHSSGQGSLDLPEALAWLWRDYDPG
                     KSAQDYEMEPSERAKPVFRVKISNRDAW"
     gene            complement(3576..4430)
                     /locus_tag="VFA54_13785"
     CDS             complement(3576..4430)
                     /locus_tag="VFA54_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS51932.1"
                     /translation="MSPNRISRRDALFMLGAAALPQARAEGGILRPTRVDHVALAVGD
                     IDKAMIFYRRLFGNEVLKDARTPRRYLRLGPCYMAIAPAAAGASKRIDHFGVGIENFN
                     AASMKSSLENAGLKVRESNVGLFVTDPDGTSVQIWADQSWKLLSNAAPEAGPKQEALF
                     HPRGMHHLAIQVNDMDRATAFYRRLFGDPGTGQGNPPQPTFQAGETRILLYNPAPNKP
                     AKIDHFSVLVDHFDAPSAVKAVQALGAKAELSRQGTLNEFFDPDGIRLQVTFPGQTYG
                     NAAPAAKK"
     gene            complement(4711..5634)
                     /locus_tag="VFA54_13790"
     CDS             complement(4711..5634)
                     /locus_tag="VFA54_13790"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HZS51933.1"
                     /translation="MARIFEQLLAAAGVATAFWAAAAIQSAQVRAAESHYQLAENWAQ
                     FPPGVTKWGAVTGVDVDAHDNVYVFHRNESMPIMVFDSHGKFLRAWGAGMFKTTHFLR
                     VDRSGYVWVTDRGEMQAFKFNSEGKLLMTLGKKGVTGDNTSPDAFNGMADVAVAKNGD
                     IFIADGEGPNTRVVKFSKNGKFLKWWGGKGTDPGQFNVPHSIAIDSKGRIYVADRSNS
                     RIQIFDQGGKFLNQWTGFGAPWGLFVKGDLIYVVDGTENNCLLIASTKDGKVLEKIEG
                     LRNPTAVAVDSHDAIYVGEVNGTDVKKFVKQ"
     gene            6007..7404
                     /locus_tag="VFA54_13795"
     CDS             6007..7404
                     /locus_tag="VFA54_13795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS51934.1"
                     /translation="MSAITFVAHRAIPVNATTAGFAYLLFVLIVASTWGFFEATVSSV
                     LATLAFNFFFFPPVGTFTIADPQNWVALFSFLTTSLIASRLSAAAKRRAADAMERQQD
                     LERLYTFSRAILLTEAAEPFPKQLAQKLAEIFGLSAVALYDRRADELYRAGPSDCEGI
                     ESQLREAALQGISFTDPARNRVITAVRLGSDPIASLALQGGPMPDSVLQGIANLAAIG
                     LERARAQELAREVETTRQTERLRTALIDAMAHDFKTPLTSIKAATTSLLANPDQAAER
                     KTQMLKIADEEADHLRELIDDAIEMARLEAADIDIKAQPSDLGEIVRDVARSMKTMID
                     DRSIEVACPPKTPEIMLDRRLIKLAIKQLLDNALKYSPPGSPVRICVQTGNSAVHVDI
                     TDYGAGISAYDQRQIFQRFYRAPSAQTIPGSGLGLSIAQRIIQAHRGDLTVASRPGET
                     TFRITLPAEQKGKQA"
     gene            7401..8099
                     /locus_tag="VFA54_13800"
     CDS             7401..8099
                     /locus_tag="VFA54_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS51935.1"
                     /translation="MSAGRILVVDDEPQIRRIMRMTLTDAGYEVDDAKTGEEALEKVR
                     SFRPDLVVLDVNMPGMGGVAACRAIRGDSNIAVVMLTVRNAEADKVEALDAGADDFVT
                     KPFSTPELLARIRAALRRVPAAQSSAAKVRAGRLEIDFAARSVSIAGRRSHLTPKELD
                     LLRYLTQHANEAVPHRELLQAVWGPDYGDQVDYLRVFIKNLRKKIEPDPEHPEYITTE
                     PWVGYRFNGTPEPA"
     gene            8353..8673
                     /locus_tag="VFA54_13805"
     CDS             8353..8673
                     /locus_tag="VFA54_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51936.1"
                     /translation="MASRLAARITLVAPQVVPHPLPLERPPVLLEWNERRLRAIAEES
                     PVETRVQIYLCRDPVRTLCAVLKPHSVVVVGGRKRWWCPAWEGKLSRVLRRAGHAVIF
                     AEAE"
BASE COUNT         1730 a         2681 c         2695 g         1835 t
ORIGIN      
        1 ttttcgtaat acagcgggtc gtcgggaatg tgcgttttac ggtagacatt cacgatcgag
       61 ccgtccgcgt caatgacggc tacgctgttg taatagacac ccgccgctct ccgctcgaaa
      121 agcggggcta ccacggcgac gcgccgctcg cgggcagcgg cggaaagagc ctcggtggtc
      181 ggtccgggaa tcggctccgc cagatcaaac agcgccgggt cctcccgctg gcagaaatac
      241 tgtgaccgga ataactccgg caggcagatc acctgggccc cattttgggc cgcctcttcc
      301 actcgcccga tggcccgcgc gagattcgcc ccggtatccg gcgagcagcg catttggagc
      361 aacgcgattt tgaattttcc cgagctctgg cgcattcgct ttgattatgc ggcaaccggc
      421 gcatgttgta aaatcaggcc ggagtcaccc gattatgctg agctacacgc gtggccccga
      481 aatcccgctc ctcgacaaaa ccatcgacca ggcgttccgg gacagcgccg cccgcttccc
      541 cgatcgcgag gcgctggtgg ttccgcacca gagagcccgg ctcgtctacc gcgaactgga
      601 ggcgcaggtg gaacgcacgg cccgaggctt ggccggcctg ggcttgcgtc ccggcgatcg
      661 cgccggcgtg tgggcgacga actgcgtgga gtgggtgttg ctgcaactgg cgtgcgcccg
      721 ggctggtctc gtgctggtga acgtgaaccc cgcttaccgc tcgcgcgaac tgggttacgt
      781 gctccgcaaa tcgggcatgc gggccctttt cttgcatgag aaagacccgc gggccgatta
      841 ccggacgatt ctggaccaag ccctgagcga ggcagccgga gaaagaccgg cgctggagca
      901 gattgtttac cttggccagg agtcgtggga gcgcatgctg gcccggccgg gccagcttcc
      961 cgcagatccg gcggcgccgg atgatgtcac caacatccaa tacacttcgg gaacgaccgg
     1021 agcgcccaaa ggcgttctgc tcacccaccg gaaccttgta aacaacgcct gggtggtcgc
     1081 tcaggccatg cgattcaccg cccaagaccg gctttgttcg cccgtgccgc tttatcattg
     1141 cttcggctgc gtgatgagct cactgctggc ggtggtagcc ggcgggacgc ttatacttcc
     1201 ctccgcgcag tttgacccgc tggccacact ctgggctatc cactccgagc ggccgacggc
     1261 gctttatgga gtgcccacca tgttcatcgc cgagctggag cacccggagt tcctccgttt
     1321 tgatttgcgt tccctgcgca ccggcatcat ggccggagcg ccttgccctg tcgaggtgat
     1381 gaagcgggtg gtcggcgaaa tgcactgccc ggaaatgacg atcgcctacg ggcagacgga
     1441 ggcgtcgccg gcggtcacga tgtcgaaggt ggacgatccg ctcgagctgc gggtctccac
     1501 gattggctgc gcgctgccta ataccgaggt gaagatcgtg gcgcccggct ccggtgaaac
     1561 ggtgcccgtc ggcgagcagg gcgagctgtg cacccgcggg tatctggtaa tgaaggggta
     1621 cgaccaggat ccggatgcga cgcagcgcgc catcgacaag gagaactggc tgcacacggg
     1681 cgatctcgcg gtcatgcagc ccaatggcta cttccgcatc acgggccgcg cgcgggacct
     1741 gattatcagg ggcggtgaga atatttatcc ccgtgagatc gaagagtttc tctacacgca
     1801 tccgaagatc gccgacgttt acgtcgtcgg gctgcccgat ttgaagctgg gcgaggcggt
     1861 gctggcctgg atcaagctca aagcgggaac ctcggccacc gaagaagaaa ttcgcgaatt
     1921 ctgccgtggc aagatcgcgc atttcaaaat cccgcaatat atccgctttg tggattcgtt
     1981 cccgatgacg gtgaccggca aagtgcagaa ataccggatt cgcgaggaag agatccgggc
     2041 ccggggcctg gaagaagcgg ccaaagtgga gacggcgtaa tcggcggaaa gagaatcttc
     2101 ggttccgcgc cggcgcgcac gtgctccact ggcgtccccg atttccgagt cctgcttata
     2161 tttaccaggc gtcccgattc gagattttta cgcgaaatac tggcttggcc cgttctgagg
     2221 gttccatttc gtagtcctgc gcgcttttgc cgggatcgta gtctcgccaa agccaggcca
     2281 gcgcctccgg caggtcgaga gagccttggc cggagctgtg catgccgtct ccgaaacgga
     2341 aatgaaaatc gtagcctcgc agcttgagcg agttggcaag ctggatattc tggagcggcc
     2401 agcttccggc gcgggggctt tcctgatctt ccgaaccgtc cgccagccac actcgaatat
     2461 tgcgcttggg ttcccggcgg atcttgaagg gatagatgtt gcccccgtcc aggtgctcct
     2521 ccggccgcca ttgcaaggct gtatagcttc caatattcga gagcacgcgg ctgaacttgt
     2581 cgggaaagta ccaggcgacg ttcagtgagc aaatccctcc cgaggactgg ccgccgatgc
     2641 cgcgcgaata gccatcgctg cggatcttat acgtcttctc gacttccggc agaatttctt
     2701 cgagaaggaa ccggccgtag cggtcggaaa cggtatcgta ttcgatgctg cgatggcgcg
     2761 taccgtcttt gggggccacg agcacatgaa tcatcggcgg aatcagcttc tggtgcacca
     2821 ggttttcggt taccgtgaag agacgcatgg cgtaaagatc gccgcgcgcc agattttgcc
     2881 cgtcctgcca caccatgagc gcggctcccc gcgagggatc atatcccggc gagacataga
     2941 cccagtattc gggattccag tcagggtaaa ttttgctggt gattgtcttc ttctccgaca
     3001 gagcgccctt cggcactccc ggctgcggat aagaatccgg cccgtagccg gcgacgtcat
     3061 accgataggc ctgccccatg gattgcccgt cggcataaaa ctggtagttg tgggttacgc
     3121 caattcgcat tttcttgacg agataccaaa gattcgaacc gggcacacgg gtcatggtct
     3181 cgggcgcgtt gccgtccaaa gaaaccgatg cagtcttgtc agtctccact gcaaacagaa
     3241 aatcctgacc ccatacggcg ctcgcgcctc gttctttcaa gtcgaacaaa gtttttggaa
     3301 gcaaatcctt gagcgaaggc gaatttgcgc gcgccgcttc gatcaaggct tgcagaggat
     3361 gcgtctggcc atgcgcggcc agaggaatca ttccaacgag cacgaaaaca aatagctttc
     3421 gcatggagct ttcctcctta cccatttatc cggtcccagc cggaattgaa gaccaaaaag
     3481 cccggaacgg gataatccat tttgtgtgca aggcatagaa ttccacggcg cctcccgcag
     3541 tttctacagt ttccgcccgc gcgcgccgag cggcgctact tcttcgccgc gggtgcggca
     3601 ttcccgtacg tttgtcctgg gaatgtcact tgcaggcgga tgccatcggg atcgaaaaac
     3661 tcattcaacg ttccctgtcg cgacaactcg gctttggcgc cgagcgcctg gacggccttc
     3721 acagccgaag gggcgtcgaa gtgatcgacc agcacgctga agtggtcgat cttcgccggc
     3781 ttattgggcg ccgggttata gagcaggatt ctggtctcgc cggcctggaa ggttggctgc
     3841 ggcggattgc cctggccggt cccgggatcc ccaaacaacc tccggtaaaa tgccgtcgcc
     3901 cggtccatgt cgttgacttg aatggcaagg tgatgcatgc cgcggggatg aaagagagcc
     3961 tcctgtttcg gaccggcttc gggagcggcg ttgctcaaca gcttccaaga ctggtccgcc
     4021 cagatttgca cgctcgtgcc atcaggatcc gtgacaaaga gcccgacatt cgactcgcgc
     4081 acttttaacc ctgcgttctc aagcgagctt ttcatgctgg cggcgttgaa attttcaatg
     4141 ccgacgccga aatgatcgat gcgcttggat gcgccggccg cggccggcgc gatggccata
     4201 tagcaggggc caaggcgcag gtaacggcgc ggcgtccggg catccttcag cacttcgtta
     4261 ccgaagagac ggcggtaaaa gatcatggct ttatcaatat ctccgaccgc cagcgcaacg
     4321 tggtccacac gggtagggcg aagaatccct ccctcggcgc gagcctgcgg cagcgccgcc
     4381 gcgccgagca taaacagcgc atcgcgccgg ctaatccgat tgggtgacat gcttcctcct
     4441 atcgccaact tttcgggccc tgcttcgaga tggccattgt acccgaagaa gcgcgcgcgg
     4501 gctcttgcac tcccggattt ctccaaaacc gagcttcgca ggcccctttt gtaagcggca
     4561 gcagcggaaa tcgccgcatt aaaatgggaa tctcaagcgc ctgcgcttgt gggtccaggc
     4621 tgcaaatggg atgggcggaa agcacccgaa gcatttcaga agcggcattt gagaagtaac
     4681 ttggcgttcc agcttcggcg gtttgatttc ttactgcttc acaaacttct tcacatcggt
     4741 cccgttcact tcacctacgt aaatggcgtc atgggagtca acggctacgg ccgtaggatt
     4801 tctgaggcct tcgattttct ccagcacctt gccgtctttc gtgctggcga tcagcaagca
     4861 gttgttctcg gttccatcga cgacgtaaat caggtcgccc ttgacaaaca ggccccaggg
     4921 ggcgccgaaa ccggtccact gattcagaaa cttgccgccc tggtcgaaaa tctggatgcg
     4981 gctgtttgag cggtcggcca cgtaaatgcg gcccttggag tcaatggcga tgctgtgagg
     5041 cacgttgaac tggccgggat cggtcccctt gccgccccac cacttgagaa acttgccgtt
     5101 tttggaaaat tttacgaccc gcgtgttggg accttcgccg tcagcgataa agatgtcgcc
     5161 gtttttcgcc accgcgacgt cggccatgcc gttgaaggca tccggcgagg tgttgtcgcc
     5221 ggtgacgccc ttcttgccca gcgtcatcag cagttttccc tcggagttga atttgaacgc
     5281 ctgcatctcg cctcggtcgg tgacccagac atacccggaa cgatcgacac gcaagaagtg
     5341 cgtcgttttg aacatgcctg caccccatgc ccggagaaat ttcccgtggc tgtcgaaaac
     5401 catgatcggc attgattcgt tgcgatggaa cacgtaaacg ttgtcgtgtg cgtccacatc
     5461 cacgccggtt acagcgcccc acttcgtgac gcccggcggg aattgcgccc aattttcggc
     5521 caattgataa tgactttcgg ctgcacgcac ttgcgcgctc tgtatagcgg cggcggccca
     5581 gaacgcggtc gcaactccag cagcagctaa aagctgttcg aatatacggg ccattcgctc
     5641 tccttgcaat ccgtcatatc ttctaaagag ccggtctgct gatcagggca cgtgaatttc
     5701 cggcgactat cttagcagac gcccgcggct ccttcacgcg ggggagggcg gcattgagga
     5761 agggattcgg gagagggaat ggcggcttcc gcagacgccg cgcgcctcgt cgcgttccag
     5821 gcacgcatgg aataagaagc ccggagcgat ttgcattcga caagcggatg ttccgatgac
     5881 accggccgca ggcggtctga tacgccgatt ggctctcggg cgtgggcatc gcctagaata
     5941 gcagccagga cgctcgtgag tggacttgcc aaagccgcat ggcgattgtt cctgtcaata
     6001 gcaggggtga gcgcaatcac atttgtggcg caccgcgcga ttccggtaaa cgccacgacg
     6061 gcgggtttcg cgtacctgct cttcgtcttg attgtcgcca gcacctgggg gtttttcgag
     6121 gcaacagtct cgtcggtcct ggctacgctg gctttcaact tctttttctt tccgccggtc
     6181 ggcactttca ccatcgccga cccgcagaat tgggtggcgc tgttcagctt tctcacgaca
     6241 tcactgattg cgagtcggct atccgcggcg gcaaagcggc gcgccgccga cgccatggag
     6301 cggcaacagg acctggagcg gctctacacc ttcagccgag ccattctttt gaccgaggcg
     6361 gcagagccgt ttccgaagca gctcgctcag aagcttgccg aaatcttcgg cttgagcgcg
     6421 gtggcgcttt acgaccggcg agcggacgag ctatatcgcg cgggtccctc ggactgtgag
     6481 ggaatagaaa gccagcttcg ggaggccgct ctccagggca tctcctttac agatccggcg
     6541 cgcaaccggg tcatcacggc agtgcggctc ggctcggacc ctattgccag cctcgctctc
     6601 cagggcgggc cgatgcccga ttccgtcctg caaggcatcg ccaatctggc cgccattggt
     6661 ttggagcggg ctcgggcgca ggagctcgcg cgcgaggtgg agacaacgcg gcagaccgaa
     6721 cggcttcgaa ccgcgcttat cgacgcgatg gctcacgatt tcaaaacgcc tctcacgtcc
     6781 atcaaggctg ccaccacgtc gctgctggcg aacccggatc aggccgcgga gcgaaaaaca
     6841 cagatgttga agatcgcaga cgaggaagcg gatcacctgc gggaactgat cgatgacgcc
     6901 atcgaaatgg cgcggctcga agccgcggat atcgatataa aggcccagcc ctcggacctg
     6961 ggggagattg ttcgcgatgt cgcccgctcc atgaagacca tgatcgatga ccggtccatc
     7021 gaggttgcct gccctccgaa gacgcctgaa ataatgctcg accggcgtct catcaagctt
     7081 gcgatcaagc agcttctgga caatgcgctg aaatattccc cgccggggtc gcctgtgcgg
     7141 atttgcgtac aaaccggcaa tagcgcggtg cacgtggata tcacggacta tggggcgggg
     7201 atatctgcgt acgaccagcg gcagattttc cagaggtttt accgagctcc ctccgcgcaa
     7261 acgatccccg gctccgggct aggtctcagt atcgctcaga ggatcataca ggcccatcgg
     7321 ggagatctga ctgtagccag ccggccaggc gaaacgacct ttcgcatcac cctccccgcg
     7381 gaacagaagg gaaaacaggc atgagcgcgg ggagaattct tgtggttgac gatgaaccgc
     7441 agatccggcg cattatgcgg atgacgttga ccgatgccgg atatgaagtg gacgacgcga
     7501 aaaccggtga agaggccttg gagaaggttc gcagtttccg cccggacctc gttgtgctcg
     7561 acgtcaacat gcccggaatg ggcggtgtgg ccgcctgccg cgccattcgg ggcgactcca
     7621 acatcgccgt cgtgatgctt acggttcgga atgccgaagc tgataaagtg gaagcgctcg
     7681 acgcaggcgc ggatgacttc gttaccaagc ccttcagcac accggagctg cttgcgagga
     7741 tacgggcagc tctccgccga gtcccagccg cccagtcttc cgccgcgaaa gtccgcgccg
     7801 gcagactcga aatcgacttt gcggcgcgca gcgtgtcgat cgcaggcagg cgttctcatc
     7861 tgacgcccaa agaactcgat cttctccgct atctgacgca gcacgcgaac gaggcggttc
     7921 cgcatcgcga actgctgcaa gcggtctggg ggcccgatta cggcgatcaa gtggattacc
     7981 ttcgggtctt tatcaagaac cttcgaaaaa agatcgaacc ggatccggag catcctgagt
     8041 acattaccac ggagccctgg gtcggctatc gcttcaacgg aactcctgaa cctgcctagg
     8101 atcgcgggca ttttcgccgg ccgcgatctt cataacttct tcatgccttt tctatgactt
     8161 cttcaggccc atcgccctaa gcttgggttg tgggaggcaa gaatctccaa tggcgcgggc
     8221 tcttgaggag attttgacgc cggccccggc gaaaccggaa ctgcgagcgc cttctgccga
     8281 ggcccggctg aacatcgatg ttgtttttac atcgatggaa gccacggccg ccgcctccag
     8341 caggcggcgg agatggcgag tcggctcgcc gctcggatca cgctggtcgc gccgcaggtt
     8401 gtcccgcatc cgctgcccct cgaaaggccg cccgtgctgt tggaatggaa cgagcgccgc
     8461 ttgcgcgcca tcgctgaaga gagtccggtg gagactcgcg tacagatcta tctgtgccgg
     8521 gaccctgttc ggacgctttg cgcggttctt aagccacact cggtcgtcgt tgtgggcggc
     8581 cgaaagcgtt ggtggtgtcc cgcttgggag gggaaattgt cgcgcgtatt gcgccgcgcc
     8641 ggccatgcag tgatctttgc tgaagcggag taagtctatg ttggacgttt tttacctcgc
     8701 actcggctgc ctggtcctgc ttggctgctg ggcctttact aaagcctgcg accggctcta
     8761 ggaaagaaaa ccatggacta catcgttgcc ggcatcgcct cagcgggctt gttgctttat
     8821 ttgatttacg cgctgctgcg tcccgagaag ttttgagcag actatgacca gcgccggcat
     8881 tttgcaaatc gcgatttttc tgagcctgat cgttctctgc gccaagccgc tcggctccta
     8941 c
//
LOCUS       DASREK010000256        23591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1448613, whole genome shotgun sequence.
ACCESSION   DASREK010000256 DASREK010000000
VERSION     DASREK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 23591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23591
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448613"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="VFA54_13810"
     CDS             complement(<1..126)
                     /locus_tag="VFA54_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS51937.1"
                     /translation="MPRLKDKVGIVTGGSAGIGQATAILFAEEGAKVTVADLDEAG"
     gene            360..728
                     /locus_tag="VFA54_13815"
     CDS             360..728
                     /locus_tag="VFA54_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZS51938.1"
                     /translation="MRILKVLAVALCLTVAIPVSFAQKGSKKAKNTKSAKATTGDVAA
                     GKDLFEAKCSVCHNADSTEKKIGPGLKGVKDGKLPSGKPATHDVIMDVLNTGGGAMPP
                     YKDMLTNEEKENVIAYVMTL"
     gene            complement(1724..2302)
                     /locus_tag="VFA54_13820"
     CDS             complement(1724..2302)
                     /locus_tag="VFA54_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZS51939.1"
                     /translation="MSFAEQYRLELLDAVNGIPLEKVEEAIRILARKREEGRSIFVCG
                     NGGSASTASHFVCDMVKGASYDRALRFRIQALTDSIPTITAYANDVSYEHVFVEQLRN
                     FAAPGDVLIAISGSGNSPNVLRAVEYANSIGCYSIGLSGRDGGRLAPLCQLSLNVPAF
                     HMGRIEEAHFTICHMISYYFMEQEPPLNQLRA"
     gene            complement(2299..3159)
                     /locus_tag="VFA54_13825"
     CDS             complement(2299..3159)
                     /locus_tag="VFA54_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZS51940.1"
                     /translation="MDVLAIDIGGTKHSLALFRDARLLRRESYPTDAAGGRSWMLDHL
                     FRLFPKWLRSSAGLAACGVGFGGPVDFALQRVSRSMHSGGWENFALAGAIEKELGIPC
                     WMDNDANLGALGEHRFGAGRGAGSLVYITLSTGIGAGILVDGKMVRGADSLAGELGHV
                     LLKEDGPLCSCGSRGCLEALCSGQAIERSAGRPAAELLADSTFRPVYVSWLARGLRVP
                     LLLLNPDRIIVGGGISKAGSAFFDDVRAELRRQMPASLPVRIDLRPAALGDDSVLWGA
                     RELARENLKQ"
     gene            complement(3172..4161)
                     /locus_tag="VFA54_13830"
     CDS             complement(3172..4161)
                     /locus_tag="VFA54_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZS51941.1"
                     /translation="MNLPDLMERFSGVRALVVGDLCLDRWCHYDPALAEASRETGIPR
                     VGIIATEVTPGAGGTVANNLVALGAGRVAVLGAIGQDGFGFELQRELAERKIDYSLVV
                     ASKAVQTFTYTKLINSVTKVEDLPRCDFVNTRPLPADVENQLIANFNAHFYEFDVILV
                     SDQAETESGGVVTDWLRGLIGDVAERYPQKLIVADSRVRIELFRNVVAKPNYEEAAAA
                     CRRAFGFVDFQRLRALIGSRPLLVTQGARGVLLVDNHGERLVPSENISNPVDICGAGD
                     SFAAGLALALRASGDFPTAIRFGNLVSSITIMKRGTGTAAPHEVLAKAAVHLS"
     gene            complement(4158..5132)
                     /locus_tag="VFA54_13835"
     CDS             complement(4158..5132)
                     /locus_tag="VFA54_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51942.1"
                     /translation="MSSRYPIFDRSRLLIKPLAERKHDMDVGYLLALDAAEPPFSHPQ
                     LPAVASRLIEARRRGSARILMLGAHVLKMGVSRHLIDLIEGGWLTHLAFNGAGAIHDY
                     ELARIGASTESVARYIQTGEFGLWRETGILNDWISEAAELDVGFGENVGRRIAESSFP
                     YKHLSVFAAAYRCGVPATVHAGIGYDIIHEHPNCDGAALGRASYHDFLIFARSVENLE
                     GGVLLSFGSAIMAPEVYLKALSMARNVAWQEGRKIRHFLTAVFDLVPITGDYHRELPK
                     TDPGYYFRPHKTILVRTVADGGESFYFCGDHRATLPALRRAILNEAGK"
     gene            complement(5140..5967)
                     /locus_tag="VFA54_13840"
     CDS             complement(5140..5967)
                     /locus_tag="VFA54_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZS51943.1"
                     /translation="MRSTTAASLECLHPGVNALNARVVLFDFDGTVSLIRSGWVDVMV
                     PMMVEILLDLNTGESEAGLRAIVEEFVGRLTGRQTMYQMIELARQVALRGGSPLDPLA
                     YKRMYYDRLSSVIGGRIEALRAGRVPPDQMMVPGARRLLETLRDRGLEMYLASGTDQP
                     YMREEARLLDVDRYFDGRVFGALDDYKSFSKKILIQRIIAGSGFHGGQFLGFGDGYVE
                     IENVKEVGGAAVGVATDEPLCIRADPVKRGRLAGAGADWIIPNYNCYDELMAALFPA"
     gene            6176..7381
                     /locus_tag="VFA54_13845"
     CDS             6176..7381
                     /locus_tag="VFA54_13845"
                     /inference="COORDINATES: protein motif:HMM:NF025934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HZS51944.1"
                     /translation="MSLFSRREFLAAIPAVQIKRASGRGQQYPSEIKRYADPLTEREV
                     WRLTSPANPHFHPYSYQRFVARNNSFLLLAGRREEGIQVFRLELPSGRLAQLTQGAGI
                     EGYSICLAPDERSVFFLQSGVLKQLSLRTLKEREIYHTEGGWWATGDMGISADGRHAA
                     VVEMQSADRVAERKADERLRLQFERRPLCRIRAVEIAKENSWIVTEEKAWIGRPQFRP
                     RRDQLLYCRIGPWEQLTSRLWLVNLDGKQKQNVRRRQGEEELGPEYWTADGRLIGYAH
                     YQSQNGRKATVRTINPDTREERVLTPCTQFWHLRGNANNSAIAGECRSKAGPNIYVVF
                     PELQREMTICEHASSGKAYVLAEDEDPLAAQPRPAFSPDSQWIYFASDRHGAPAVYRA
                     GVADMVEKT"
     gene            complement(7465..8601)
                     /locus_tag="VFA54_13850"
     CDS             complement(7465..8601)
                     /locus_tag="VFA54_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZS51945.1"
                     /translation="MKLRPILLAVLLIGCFVYLTTARRWPEVDFGRNAKLWTSLEPAQ
                     SAGLTPEENINISIYRMASPATVFITSTVYQRDWFFQVFPVRDSGSGFLIDAEGRILT
                     NYHVVSGNAKVIEVTLGDKMKYNARILGYDQRNDMALLKIQPKGRLPFLKPGDSSNLQ
                     VGQKVLAIGNPFGLEGTLTTGIISSLGRTIRDENGRPLEDMIQTDAAINPGNSGGPLL
                     DSQGNVIGINTAIYGPGANIGIGFAIPINRAKAMLSDLASRGRVSRPLLGVTTVFIPA
                     EFAEALDLPARPGLLIQQVEPGSAADQAGLRGANREVIVGNYVVEIGGDFIVEVEGQP
                     VSNRSVLTRALERKKAGDPLRLGIIRGRRQLTVTVTLGESPQQL"
     gene            complement(8598..9215)
                     /gene="coaE"
                     /locus_tag="VFA54_13855"
     CDS             complement(8598..9215)
                     /gene="coaE"
                     /locus_tag="VFA54_13855"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZS51946.1"
                     /translation="MRGKILKVGLTGGLATGKTHVGRVLAGLGCHVLSADELGHQVLL
                     PGGEAYNDVVKEFGPGILAADGRIDRRLLAAQVFDRPERLEKLNSLVHPHVVKREELW
                     LSELEARQPGAIAVVEAAILIETGSYKRFDRLILTVCSPELQMRRHMERSGDSEAETR
                     ARLARQMPLEIKRRYADYVIDTSGSREDTDRQVREVFAKLRQDAP"
     gene            complement(9230..10168)
                     /locus_tag="VFA54_13860"
     CDS             complement(9230..10168)
                     /locus_tag="VFA54_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HZS51947.1"
                     /translation="MAARLLNGNAIRDQILSELRTEVAGMTAAGCRPGLAAVLAGHNP
                     ASEVYVRNKMKACESVGIYSEKITPPETVSTGEMLDLVARLNARDEIDGILVQLPLPA
                     QVDSKRVLLAVDPAKDVDGFHPFNIGLLVTQRPGLVACTPAGIMEILKRSRIPVSGRR
                     AVVLGRSDIVGKPVAFLLLHENATVTICHSRTENLAAIAREADILIAAIGRPAMVTRD
                     FIQPGATVIDVGTNRLENAADVERIFAGMPSKIEQFREKGSLLVGDVHPVDAIETAGA
                     YTPVPGGVGPLTVSMLMANTVKAARLRRGGCGVLRH"
     gene            10314..12476
                     /locus_tag="VFA54_13865"
     CDS             10314..12476
                     /locus_tag="VFA54_13865"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZS51948.1"
                     /translation="MSPLHLSLLIHAHQPLGNFESVQEDVYRLAYLPFVEELARHPAI
                     HIALHYSGILLAWLEKKHPEYLEQLRDLAGQGQVEILGGGYYEPVLSAIPDRDRLAQI
                     ERLSDFLEKRFGSRPRGAWLTERVWDPTAPMTLAQSGIEYTLTDDYHFLSAGLEADQL
                     YGYYLSEWQGHAVKVMPGQKSLRYLLPFRMEADAIAYLRSIAERRPGGLVAMGDDLEK
                     FGAWPHTHEHVYRNGWLRRFFEAIEQSSEWLKTTLAGDYVREHEPLGRIYLPTASYPE
                     MMGWALPPKASLDYEDLVHRVEKLPEAAALGRFVHGGVWHNFFYKYEEANHMHKRMLE
                     LSRRYEDLDKSTPPGVPARGLYEEGYEHLLSAQCNDAYWHGVFGGLYAPHLRTSVYRA
                     LLRAENIAEKIDSSPRTTRRWDFNLDGADEIFLANERLALIVAAGDGGTAAEIDFRPR
                     AFNAINSLRRRPEAYHARLQDAQRGEAGGAVSIHDRMAAKESGLECYLQYDRYNRHSF
                     RSLVFAAGRGLEDYRAGRLDESPELAGGRFSVLQLARDRCVLAGRDRQRLCRVRTEIS
                     LAGPNVAVNWSLSQANAEELEAGLELVLNFLAPDARDRYFLLPAGLGDAGGPDHPETC
                     LEAIASPSFTRQKLHWAGEAAGSCIALLDEWLDVRVRINVTPPAKWWITPIFTVSQSE
                     GGFEKVYQGSCLLPHWRVQDGELQATVRLEFGATGERS"
     gene            12473..13837
                     /locus_tag="VFA54_13870"
     CDS             12473..13837
                     /locus_tag="VFA54_13870"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HZS51949.1"
                     /translation="MTKAEQLAQFVVSAGYDRLSEAAREQLKIRILDAVGCAIGALGA
                     EPIRRVRQHIEDFGGGVQSGLIGGGRTAPDRAAFYNSSLVRYLDFNDSYLAKGETCHP
                     SDNLGAVLAAAEYAGAGGREILTALAVAYQVQCRLSDVAPVRAKGFDHTTQGSYAAAA
                     GVAKALGLDAQSTMNAIAISGTAFNALRVTRTGALSNWKGLAYPNTAFCCTHAAFLAM
                     RGITGPAEVFEGNKGFMDAIAGRFEIDWSKEDLERVRRTIVKKYNAEIHSQSAIEAAL
                     ELKQEHRLTGREIQRIEIETFDVAYNIIGGGEEGDKIQVRTKEEADHSLPYIVAAALL
                     DGQVMPDQYTPERIGRADVQTLLRKIRVRPREAYSQRFPEEMPCRIQLILENGETLVK
                     EKRDYEGFHTRPARWEAVLEKFERLSQRHAAPALRSGIADQIANLESARTAGELMALL
                     AQVS"
     gene            complement(13902..14432)
                     /locus_tag="VFA54_13875"
     CDS             complement(13902..14432)
                     /locus_tag="VFA54_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(14223..14225),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZS51950.1"
                     /translation="MKISSRLFAGSCLLAMSLGSAAAATHLKVGDMAPDFTLPSTTGK
                     PIHLADYRGKKNVVLAFFVLAFTGGUTKEMKAYQAGLAKFEANNTQVLGISVDSIPTL
                     QHWAQELGVGFPLLSDFVNRKVATEYGVFNPERGFATRATFVVDKEGKIQHIEEGQTA
                     IDPSAADTACSRLEHK"
     gene            complement(14485..15744)
                     /locus_tag="VFA54_13880"
     CDS             complement(14485..15744)
                     /locus_tag="VFA54_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS51951.1"
                     /translation="MRIVLQSSVSFLALSLCVAWAQRPPAGQQGGASSTAPAGPTAPL
                     PSPGRGQPGIGQPSQQDRFPSEQRPIFFSGKVMIEGGLPPPEPVTIERVCNGRPRPEA
                     YTDSKGRFSFQLGQQTETMMDASVGDVDVGPARGRYGRDRNSANGAFGPLDSSNPGVV
                     NLMGCELRASLPGYRSDSIMLSRRSIFDNPDVGTIILHPLANVQATAISITTLEAPKD
                     AKKAWEKAQKLLQQKNPKPAEAAKELEKAVQVYPKFAAAWCMLGESRLALKDEEGARK
                     AFEQSLAADSKYARPYMQLASLEMRASRWDSAADITSRLSQMNPYYTQAYYINAIANY
                     NLGKMELAERSARNALKGEAAGRNPALHHLLGSILAREGNFPSAAGEFRSFLQLSPDS
                     PAAEQIRKQLAEWESLNVIPHQENAVK"
     gene            complement(15855..16670)
                     /locus_tag="VFA54_13885"
     CDS             complement(15855..16670)
                     /locus_tag="VFA54_13885"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HZS51952.1"
                     /translation="MTYIPPKAYRNIAFLNSYHARPLRILAEYLEPFARFREEKVYDT
                     IVFFGSARVCDRETALQCIRKARSALTRKQAQRLLKTSAYYEAARELARMLTEWSMAL
                     SSEPARFVVCSGGGPGIMEAANRGAADAGGRSIGLNISLPFEQLPNPYITRRLSFDFH
                     YFFMRKYWFAYLAKALIIFPGGYGTCDELMEILTLNQTGKFKKKMLVLIYGRKYWDEV
                     LSFDAMQKWGMIDEQDRQWIQYADTPKTAFRLITNWLTVNYPPNQKGNPALSR"
     gene            complement(16680..17801)
                     /locus_tag="VFA54_13890"
     CDS             complement(16680..17801)
                     /locus_tag="VFA54_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51953.1"
                     /translation="MGWTGVWATLVTALLFLNSAAAADRWDLQYFYDKNDSSLAFTDL
                     SFSSPLHGAALGVVAGRDRQRPVLALTYDGGRNWTLVSLKEAVVSLFFSNDQTGWLAG
                     AKNHLWKTQDGGRTWKSAAPKGVRADVLRVFFLDESRGWLLCSQKQIYSTNDGGRSWQ
                     LLEASRRPDPPDASTFYTWAAFQQETGLLTGWSRPPDPRSEILEWIRPELRPPSRHPT
                     TAVFLHTADGGRSWNPSVLRNFGEIVRARILPSGAAALLLIQRPDSLASPSAIVELDL
                     KTLQSHPVYQNRSRWLTDLALLGPDTTLAAAVDQEGRAPFPAIPSKLKILRSVGFNNW
                     TEMQVDYRAEARAALLAAPDSTHAWVATDTGMILTLVKD"
     gene            17855..19060
                     /locus_tag="VFA54_13895"
     CDS             17855..19060
                     /locus_tag="VFA54_13895"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HZS51954.1"
                     /translation="MRLAAVLLWAVAIGSGAAAELPAGAEIQVRLQTAAGSSKSKAGD
                     VVRATVIVPVLAGGDILIPAGSTITGKVKGAQPAKGGTERAALELEFGELLRPGDQQA
                     TQLQTKIAAVDNARETVDGSGRIVGIAASETLSAQMDKGLSKLEQRAAGLAGFLEAAK
                     EAVLKQADVEIDYEPGVEVTLQLTSSVKLNAAQLAPGNAPQPIEDAAGLAELVNREPF
                     QTMAEKPPRPSDIINLMFIGGREQIEAAFAAAGWATAEALNARSGMETFRAIAEGRGY
                     QQAPVSTLLLEGRKPDLVFQKQNNTFAQRHHMRVWMRRDQFHGQAVWVAAATHDIGIE
                     FSPENRTFIHKIDSQIDRERAKVVNDLLVTGKVRGLALVERPGAPRESQNATGDKLET
                     DGSMAVLQF"
     gene            complement(19095..19688)
                     /locus_tag="VFA54_13900"
     CDS             complement(19095..19688)
                     /locus_tag="VFA54_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZS51955.1"
                     /translation="MGQRQKDLEARLARTEQQLRLFQKISRFMVRNLSLQEVLQGIVD
                     LVAEFMQCDSCLIYLLDREELVLCASNNPHPSEIGKIRLRLNEGLTGWVARERRLLSI
                     SKECYRDPRFKFFGSLPEDTFEAFLSVPVISRDKVVGVINVQHRLPHQHTGSEMELLT
                     TVGELVGCALVLARMASERVHDASHVDLVLSSFPPKP"
     gene            19831..21726
                     /locus_tag="VFA54_13905"
     CDS             19831..21726
                     /locus_tag="VFA54_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HZS51956.1"
                     /translation="MNLPATARSLSEVHSSIEVPDLSLWRRMFAFAGPAYLVSVGYMD
                     PGNWATDLEGGARFGYQLLWVLVMSNAMAILLQTLSARLGIVSGKDLAQACREHYPQA
                     VGFALWAISEVAIAACDLAEVLGTAIGLQLLFGIPLITGVLITALDALLVLWLQRFGM
                     RVLEAFILSLITVIGVCFLIEIVLAKPGLKEMAGGLVPRLNGESLYVAISILGATVMP
                     HNLYLHSALVQTRRIDSSEKAKQAACRYNLVDSVVALNAALLVNAAILVMSAAVFFSR
                     GIAVREIQQAHKLLEPLLGTTLASGAFAIALIASGQSSTITGTMAGQIVMEGFLSIRM
                     RPWVRRLVTRALAITPAALVIGFAGEGATLKLLLLSQVILSLQLPFAVIPLIHFTSDR
                     SRMGGFANRNWVKLLAWAAAAIIVGLNVQLVWTTLAGWVSTSGRWSPAIEFGVIPAVA
                     ALGLLLVWVTAEPWIRRQAPRREAVTMPAIEAGAREPAYQRILVTLDHSALDRQAIAH
                     AAAIARTGAARGARIYLVHVEEGAPSRLYGNLASDAEVRAGEDYLESVRRTLEAQGIP
                     TEGFIAHSSNPGEEIVRVAREVGADLLVMGAHGHRKLKDFLLGDTIEPVRHALDTPIL
                     IVRQSPK"
     gene            21847..22683
                     /locus_tag="VFA54_13910"
     CDS             21847..22683
                     /locus_tag="VFA54_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZS51957.1"
                     /translation="MNTVEFTGLVFLVAGVAGFLGSLTGLGGGVVITPVLTLLFGVDI
                     RYAIGATLVSVIATSSGAASAYVQEGFSNIRIAMFLEIATAGGAICGALLAAKTPTSA
                     LAVILGAVLLYSAYRSARAHSDEVSAQAPDALAVRLKLDSSYPTPAGRQHYHVRGVKS
                     GFGIMALAGIISGLLGIGSGALKVIAMDQFMRLPFKVSTTTSNFMIGVTAAASAGVYL
                     RRGYIDPALAMPVMLGVLAGSVLGARELTQTKSRILRVVFGLVVAALAVEMIYGGATG
                     RI"
     gene            22687..23064
                     /locus_tag="VFA54_13915"
     CDS             22687..23064
                     /locus_tag="VFA54_13915"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HZS51958.1"
                     /translation="MIHGPSEERVQQVIGMLLRLGVMIAAGVVAAGGMCYLVESGAAR
                     PEYHEFHGIAEDLRTVPGVWRGVLDLRCKSIIQLGILMLIATPIARVAFSAAAFGLQR
                     DWTYTAITSIVLTILLYGLVWGG"
     gene            complement(23088..>23591)
                     /locus_tag="VFA54_13920"
     CDS             complement(23088..>23591)
                     /locus_tag="VFA54_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZS51959.1"
                     /translation="ASTDRVFRTIELAVLGLQQLGLAGGRIAVAGLNPHCGENGLFGV
                     EDREQIVPAVEQAVARGWNVAGPISPDTVFHRAVMGEFAMVVAMYHDQGHIPSKLIAF
                     QETVNVTLGLPIIRTSVDHGTAFDIAGKNLADPTNMICAIEMALRLIAGRSARGAAAG
                     AAPTLAS"
BASE COUNT         4920 a         6686 c         7555 g         4430 t
ORIGIN      
        1 cccggcttca tccagatcgg cgaccgttac tttggctccc tcttcggcga acagaattgc
       61 ggtagcctgg ccaatgccgg ctgagccgcc ggtcacaatt cctactttgt cttttaagcg
      121 tggcatgtgg tctcagggtg aatgtgctgg ccggaggcaa gccgcaggca ttcgaaagcg
      181 aagattatag cgcgagcctg cggccggcat gacagaaaag caggtccgca gcaggatgca
      241 ggggcggccg ggccgcggcg gtttcggcgg tgggaaaagc agcgccaaga gggagggaac
      301 aatggcaggt ttcagacatt gtaggttaga atgatggttt tccgggagga atcgacgaaa
      361 tgaggatatt gaaagttctg gcggtcgcgt tgtgtctcac ggtggctatt ccggtctctt
      421 ttgctcaaaa gggatccaag aaagccaaga acaccaaatc ggcgaaggcc accacgggcg
      481 acgttgccgc cggcaaggac ctcttcgaag ccaaatgttc ggtttgccat aacgcggaca
      541 gcacggaaaa gaaaatcggc cctggattaa aaggcgtaaa agacggcaag ctgcccagcg
      601 gcaagccggc gacgcacgat gtcattatgg acgttctcaa tacgggcgga ggagcgatgc
      661 ctccctacaa agacatgctt accaatgaag aaaaggagaa tgtcatcgcc tacgtaatga
      721 ccctctaggc gattcccggg ccaattccct tcgttttacg gcatttcccg cggggggcct
      781 cccgaattcg aaggcgccac gagcgcagct tggtatagtt acagatagcg ccgagcacag
      841 gatagcgggc cggttggtgg atcgccacag gttcgccggc ctccggcatc acgctattgc
      901 tttcgctgat gtcaggccac agataagttg aaacgccagc gtggcctttt tccagaaccg
      961 gcacatcgtt gcggccgccg gtggcccggg taaaagaaag attcgtgtta tcgcattatt
     1021 aggagcgtgg acggcgtcgc ccgcttgggg tccgctccgg agtccgagaa gtcaacttgc
     1081 gagggcggcc tcgaagaggc tcgccgcatg ccagccgcgc cagccgctcc aaatgcagta
     1141 tcacgcggtc gatcatttcc aattccgcct gaagttgctt gataaacgcc gtaagcgtcc
     1201 gctgatccat tgccgaagcg ccttattgga tgccgattgc gccgatattc aaacgcaggc
     1261 gccgctcgaa actccttcaa aaactaacac aggtctgatg agtattacca gtatctcagg
     1321 taattttagt atgaacttag ggtactcatc tcctaagcct tacggctact actaaggtcg
     1381 tacgacgctt tccgcgagcc cggagtacta cttctccatt ggcaggcgcc cggggccccg
     1441 ggaaaatagc aatcgtattt tgggcaatga cggctcccat gggaaaaggg gaagttgtcc
     1501 gcaacctgaa agagcaactg aaattgctga acagcgctat ccaagatctg gaacggctgg
     1561 ctgcattacg aagagcgccg ccctatcgaa acgcgctccg gcagctacag gcaacccgga
     1621 agagcgtcaa gcggcgtttg ggggcgttcc ggaagaagag ccagtgaaaa gccgtccgaa
     1681 tcccggcccc ggaaacagag cagtgcggag cgcaagccgc agtttaagcc cggagctggt
     1741 ttagaggcgg ctcctgctcc atgaagtaat agctgatcat atggcagatt gtgaagtggg
     1801 cctcttcaat gcgccccata tgaaacgcgg ggacgttcag gctcaactgg cacagaggcg
     1861 ccagccggcc gccgtcgcgg ccagaaaggc cgatggaata acagccgatc gagttcgcat
     1921 attcgaccgc gcggagcacg ttgggtgaat tgccgctgcc gctgatggcg atcaagacat
     1981 cgcctggggc ggcgaaattg cgcagttgct ccacgaagac atgctcatag cttacgtcat
     2041 tggcataggc ggtgatcgtc ggtatggaat cggtgagcgc ctggatgcga aagcgcaggg
     2101 cccggtcgta gctggctcct ttcaccatat cgcaaacgaa atgggaggcg gtggaagcgc
     2161 tgcccccatt gccgcatacg aaaatcgaac ggccttcctc gcgcttgcgg gcgaggatcc
     2221 gaatggcttc ctcgaccttc tccaggggaa tcccattgac agcgtcgagc aactccaagc
     2281 ggtattgctc cgcaaaactc attgcttcaa attctccctt gccagttccc gagctcccca
     2341 gaggacggag tcatcgccga gggccgcagg ccggaggtca attcggaccg gcagcgaagc
     2401 tggcatctgg cggcgcaact cggcgcgaac gtcatcgaag aaggcgctgc ccgccttgct
     2461 gatgccgccg cccacgatga tgcggtcggg attcagcaga agaagcggca cgcgcagccc
     2521 gcgggccagc cagctcacgt agacgggccg gaacgtagaa tccgccagca gttcggccgc
     2581 cggccggcct gcgctccgct cgatggcctg gccggagcac aacgcttcca ggcacccgcg
     2641 cgagccgcac gagcataacg gcccatcttc cttcaggagc acatggccca attcacctgc
     2701 cagtgaatcg gccccgcgaa ccatcttacc atcgacaaga atgcccgctc cgatgccggt
     2761 ggaaagcgtg atgtacacca gggagcccgc gccgcgtcca gcgccaaagc ggtgctcgcc
     2821 gagagctccg aggtttgcgt cgttatccat ccagcagggg atccccagct ctttttcgat
     2881 ggcacccgcc aaggcaaagt tttcccaacc tccggaatgc atcgagcggc tgacgcgctg
     2941 caaggcgaag tccaccgggc cgccgaagcc gacgccgcag gcagccagcc ctgccgagct
     3001 gcgaagccac ttcgggaaaa gacgaaagag gtgatccagc atccaagagc ggccgccggc
     3061 ggcgtcagtc ggatagctct cccggcggag gagacgggcg tcccggaaca gcgcgagcga
     3121 atgtttcgta ccgccgatat cgatggctaa aacgtccatg ctcgggcggg atcagcttaa
     3181 atggacggcg gccttggcaa gaacctcgtg cggagcggct gtgccggtac cccttttcat
     3241 gatggtgatg ctcgacacga ggtttccgaa acggatggcc gtagggaaat cgccgctggc
     3301 gcgcaaagcg agagccaagc ccgccgcaaa gctgtcccct gctccgcaga tatcgaccgg
     3361 atttgagata ttttccgaag ggaccaggcg ctcgccgtga ttatctacca gcaatacgcc
     3421 acgcgcgcct tgggtgacca gcaagggccg gctgccgatc agtgcgcgca gccgctggaa
     3481 atccacgaaa ccgaaggcgc ggcggcaggc ggcggccgct tcctcgtaat tcggttttgc
     3541 cacgacgttc cggaacaact cgatacggac gcgagaatca gccacaatca gcttctgggg
     3601 atagcgctcc gcgacatcgc caatgaggcc tcgcagccag tcggtgacga cgccgccgga
     3661 ttccgtttcg gcctggtcgg aaaccagaat gacgtcgaac tcgtaaaagt gggcattgaa
     3721 attggcaatg agctgatttt cgacatcggc cggcagcggc cgcgtgttga cgaaatcgca
     3781 gcgcggcagg tcctccactt tggtgacgga attaatcagc ttagtgtagg tgaacgtctg
     3841 gacagccttg gatgcgacca ccaggctata gtcgatcttc cgctccgcca gttcccgctg
     3901 caactcgaat ccaaagccat cctggccgat ggcccccaga acggcgaccc gtcccgcgcc
     3961 gagagccacg agattgttag ccaccgtacc gccggcgccg ggggtcactt cggtggcgat
     4021 gatgccgacg cgcggaatgc cagtttcccg ggaggcttca gccagggcgg gatcgtaatg
     4081 gcaccaccgg tcgagacaaa ggtcgccgac gaccagggcg cgaacgccgg aaaacctctc
     4141 catcaggtcg ggaaggttca ttttccggcc tcattgagaa tcgcccgccg cagggcgggc
     4201 aatgtggcgc gatgatcgcc gcaaaagtaa aaactctctc ccccatcggc caccgttcgg
     4261 accagaatcg tcttatgagg ccggaaataa tagccgggat ctgttttcgg caattcgcga
     4321 tgatagtcgc cggtaatggg gaccaggtcg aagacagccg tcagaaaatg gcggattttc
     4381 cgcccctcct gccaagccac gttgcgggcc atcgacaggg ctttcagata gacttcgggc
     4441 gccataatcg ccgagccaaa gctgagcagg acgcctccct ccagattctc gaccgatcgc
     4501 gcaaagatca ggaaatcgtg ataactggcc cggcccaggg cggcgccgtc gcaattggga
     4561 tgctcgtgga tgatgtcgta tccgatcccg gcatgcacgg tggcggggac gccgcagcga
     4621 taagcagcag caaagacgct caagtgctta taaggaaagc tgctttcggc gatccgccgt
     4681 ccgacgtttt ctccgaaacc gacatcgagc tcggccgctt ccgagatcca gtcgttgagg
     4741 atccccgttt ctcgccacaa gccaaattct ccggtctgga tataccgtgc cacgctctcg
     4801 gtggatgcgc caatgcgggc caactcgtaa tcatgaatgg cgccggcgcc gttaaaagcg
     4861 aggtgcgtga gccacccgcc ctcgatcaaa tcgatgagat ggcgagacac ccccatcttg
     4921 aggacgtgtg ctcccagcat gagaattcga gcactgccgc ggcggcgggc ttcaatcagg
     4981 cgggaggcga ccgccggcag ttgcgggtgg gagaaaggcg gctcggccgc atcgagggcg
     5041 agcaaatagc ccacatccat gtcatgcttg cgctcggcaa ggggcttaat cagcaggcgt
     5101 gagcggtcga agatcggata tcgggagctc atgtaaaact cacgcaggga acaaggcagc
     5161 catcagctca tcgtagcaat tgtaattcgg aatgatccaa tcggctccgg ccccggcgag
     5221 gcggccccgc ttgacaggat cggcccgaat gcacagcggc tcatcggtcg caacaccgac
     5281 cgccgctcca cctacttctt ttacattttc gatctcgacg tagccgtccc cgaagccgag
     5341 gaactggccg ccgtgaaagc cgctgccggc gataatgcgc tggatgagga tctttttgga
     5401 aaaacttttg tagtcgtcca gcgcgccaaa aacacggccg tcgaaatagc ggtccacatc
     5461 gagcaaccgc gcctcttccc gcatatacgg ctgatcggtg ccgctggcca gatacatttc
     5521 gaggccgcgg tcccgcagcg tttccagcaa gcggcgcgcg ccgggcacca tcatttggtc
     5581 cggcggcacc cggcccgcgc gcaaggcttc tatacggccc ccgatcacgc tggagaggcg
     5641 gtcgtaatac atacgcttat aagccagcgg atcgagcggc gagcctccgc gcaaggcgac
     5701 ctggcgcgcc agttcaatca tctgatacat cgtctggcgc ccggtgaggc ggccgacaaa
     5761 ctcctcgacg atcgcgcgca acccggcttc gctttccccg gtgttcaagt ccagcaggat
     5821 ctcgaccatc atcgggacca ttacatccac ccagccggag cgaatcaggg atacggtgcc
     5881 gtcgaaatcg aacaggacaa ccctggcgtt gagcgcattc acgcccgggt gaaggcattc
     5941 cagggaggcg gctgtcgtcg aacgcaagtt tcctttttag cagatttgct gcggcacgcg
     6001 ccatgccgtc cggcttcgga ggcgataagc cggcggacgg aatcgccgcg cgcccatctt
     6061 ccgaggctgc tctggccggc ggcgctgcga gcgctgtccg gctatgtttt ttcttcgagc
     6121 gccgagcggc gcactggaaa tcatgccgca acggcggttt gttaagattt tgcttatgag
     6181 cttgttcagt cggagagaat ttctggcggc gattccggct gtacaaatca agcgggcatc
     6241 cggccgcggg cagcaatatc cttccgagat caaacgatac gccgaccctc tgacagagcg
     6301 ggaagtatgg cggctgactt ctcccgccaa cccgcatttt catccgtaca gctaccagag
     6361 gttcgtcgcg cgcaataact ccttcctctt gctggcgggc cggagggaag aaggcatcca
     6421 ggttttccgt ttggagctgc cgtcgggccg gctggcgcaa ttgacccaag gtgcggggat
     6481 cgaaggctac tccatttgtc tggccccgga cgaacggtcc gttttcttct tgcagagcgg
     6541 agtcttgaag caattgtcgc tgcgaacgct gaaggagagg gagatctacc acacggaggg
     6601 gggctggtgg gcaaccggag acatggggat ctcggctgac ggccgccatg cggcggtggt
     6661 cgagatgcaa agcgccgacc gggtcgcgga gagaaaggcg gacgaacgtt tgcgattgca
     6721 gtttgagcgc cggccgctgt gccggatccg ggcggttgaa atcgccaagg aaaacagttg
     6781 gattgtcacc gaggaaaaag cctggatcgg gcggccgcag tttcgcccgc gccgcgacca
     6841 actgctctac tgccggatcg gtccgtggga gcagttgacg tcccggctat ggctggtgaa
     6901 tctggacgga aagcagaagc agaacgtgcg gcgcaggcag ggcgaggagg agttgggtcc
     6961 ggagtactgg acggcggatg gcagactgat cggctatgca cattatcaat cccagaacgg
     7021 ccgcaaggcg accgtgcgca ctatcaatcc ggatacgcgc gaagaacggg ttttgacgcc
     7081 gtgcacgcag ttttggcacc ttcgcggcaa tgccaataat tcggccatcg ccggtgaatg
     7141 ccggagtaaa gcgggcccga acatttacgt tgtgttcccc gagctccaac gggaaatgac
     7201 gatttgcgag cacgcgagca gcggaaaggc atacgtgctg gccgaagacg aggatccgct
     7261 ggcggcgcag ccgcgtcccg ccttctcgcc cgacagccaa tggatctact tcgccagcga
     7321 ccgccacggc gcccccgccg tctatcgagc cggcgtggcg gatatggtgg aaaagacata
     7381 gccagcggct ctacagacgg ccgatggtaa agccggccgg atctgttgcc tctcattggg
     7441 ccgtatttca gttcgagccg cgcgtcagag ctgctgcggg gattcgccga gcgttacagt
     7501 caccgtgagc tggcgccttc cgcgaataat gccgaggcgc aaggggtcgc cggccttttt
     7561 tcgttccaga gcgcgcgtaa gcacgctgcg gttgctcaca ggctggccct cgacctcgac
     7621 gataaaatcg ccgccgattt ccaccacata gttgccgacg atcacctccc ggttggcgcc
     7681 ccgcagcccg gcctgatcgg ccgctgagcc gggctcgacc tgctggatga ggaggccggg
     7741 acgggcgggc agatccagcg cctcggcaaa ttctgcggga atgaagactg ttgtcacgcc
     7801 aagcagaggc cggctgacgc ggcccctgct ggccaagtcg gagagcatgg ccttcgcccg
     7861 gttgatgggg atcgcaaagc caatgccgat attcgcgccc ggcccgtaaa tcgcggtgtt
     7921 gattccaatc acattgcctt gagaatcgag caaggggccg ccgctgttgc cgggattgat
     7981 cgcggcgtca gtttggatca tgtcttccag cggccggccg ttttcatccc ggatggtgcg
     8041 gccaagcgaa ctgatgatgc ccgtggtaag cgtgccctcc aggccgaacg gattgccaat
     8101 ggcgaggacc ttctgaccca cctgaagatt gctcgaatcg ccgggtttca ggaacggcag
     8161 cctgcctttg ggctggatct tgagcaaggc catgtcattg cgctggtcat agccgagaat
     8221 gcgggcgttg tatttcatct tgtcgccgag cgtgacctca atgaccttcg cgttgcccga
     8281 gaccacatgg tagttggtga ggatgcggcc ttcggcgtcg atgagaaagc cggagccgga
     8341 atcccggacg ggaaacacct ggaaaaacca atcgcgctga tagacggtcg aagtgatgaa
     8401 gaccgtggcc gggctggcca tgcggtaaat ggagatgttg atgttctcct ctggggtaag
     8461 gccggcggat tgcgcaggct caagcgaggt ccagagcttt gcgttcctgc cgaagtccac
     8521 ctcgggccag cgccgggcag tggtgaggta gacgaagcat ccaatcagga gaacagccaa
     8581 aagaatgggt ctcagtttca tggagcatcc tgccgcaatt ttgcgaagac ctcgcgcacc
     8641 tggcggtccg tgtcttcgcg agaaccagaa gtatcgatga cgtaatccgc gtaacggcgc
     8701 ttgatttcga gcggcatctg ccgcgccaac cgggccctgg tttcggcctc ggagtcgccg
     8761 ctgcgctcca tgtgccgccg catttgcaac tcaggggagc acacggtcag gatcagccgg
     8821 tcgaagcgct tgtagctgcc ggtttcaatc aggatcgccg cttccacaac ggcaatggcc
     8881 ccaggctgcc gggcttcgag ttccgacaac cacagctctt cgcgctttac gacgtggggg
     8941 tgcacgagcg agttcaattt ttccagccgc tcgggacgat caaagacctg ggcggccagt
     9001 aaccggcgat cgatccggcc atcagcagcg aggatgcccg gaccgaactc cttaaccaca
     9061 tcattgtacg cctcgccgcc cggcagcaag acctgatggc cgagttcatc ggcgctgagg
     9121 acgtggcagc cgaggccagc gagcacccgc ccgacatggg ttttgccagt cgcaagtcca
     9181 ccggtcaagc cgactttcag aattttgccg cgcacacagt ctcagcggcc taatgccgca
     9241 ggacgccgca gcctccccgc cggaggcgcg cggctttcac ggtattcgcc ataagcatgg
     9301 ataccgtcag cggtcccacg ccacccggta caggggtgta ggcgccagca gtctctatgg
     9361 cgtccacggg atgaacatcg ccgaccagca aagaaccttt ttcccggaat tgttcgattt
     9421 tggagggcat tcccgcgaag atccgctcca catcggcggc gttttcgagc cgattggttc
     9481 caacatcgat cacagtagcg ccgggctgga taaagtcgcg ggtgaccata gccggccggc
     9541 caatggcagc gatcaggata tcagcttccc gggcgatcgc ggcaagattc tccgtccggg
     9601 aatgacagat ggtcaccgtg gcattttcgt gcagaagcag aaaggccacc ggcttaccga
     9661 cgatatcgct gcgcccaagc accacggcgc ggcggccgga aacaggaatg cggctgcgct
     9721 tgagaatttc cataatgccc gccggcgtac aggccaccag ccccgggcgc tgggtcacca
     9781 gaaggccgat gttgaaggga tgaaatccat cgacgtcctt ggccggatca accgccagca
     9841 ggacacgttt ggaatcgacc tgggccggga ggggcaactg caccagaatg ccgtcaatct
     9901 catcgcgggc gttgaggcgg gcgaccagat cgagcatttc cccggtgctg accgtttcgg
     9961 gcggagtgat cttttcgctg tagattccga ccgattcgca ggccttcatc ttgttgcgga
    10021 catagacttc ggaggcaggg ttgtgacccg ccaaaacggc ggctaaaccg ggccggcagc
    10081 cagcggccgt cataccggct acctctgtac gcaattcaga aaggatctga tcgcggatcg
    10141 cgttgccgtt gagaaggcgc gcggccaaaa agctctcccg aagaaacgct ctgcaaaaaa
    10201 ataatagcat gcggcatgga gtgtttccgc agatcgaggg gaaacagcgg tggaactgac
    10261 aaggcggaaa ccagtgacgt attctgtaga agacggttcc aaatcgcctc gctatgtctc
    10321 ccttacatct ctctctgctg atccacgcgc accagccgct cggaaatttc gaaagcgtgc
    10381 aggaggacgt gtaccgattg gcttacttgc cgttcgtcga ggaactggcg cggcatccgg
    10441 caatccacat cgcgctccat tattcgggta ttcttctggc gtggcttgaa aagaagcacc
    10501 cggagtatct ggaacaattg agggatctgg ccggccaggg gcaagtggaa attctgggcg
    10561 gcggctatta cgagccggta ttgagcgcaa ttcccgaccg cgaccggctg gcgcaaatcg
    10621 agcggctcag cgattttcta gagaagcgct tcggctcgcg gccgcggggc gcctggctga
    10681 cagagcgcgt ttgggatccg acggcgccga tgactctggc gcagtccgga atcgaataca
    10741 ccctgacgga cgactatcac tttttgagcg ccggcctcga ggccgatcag ctttacggct
    10801 actatctgag cgagtggcag ggccacgcgg tgaaggtcat gccgggccaa aagagcctgc
    10861 ggtatctgct gcctttccgc atggaggcgg acgcgatcgc gtatttgcgt tcgatagcgg
    10921 agcggcggcc cggtgggctc gtggccatgg gggacgattt ggaaaaattc ggcgcctggc
    10981 cccacacgca tgaacacgtc taccggaacg gatggctgag gagatttttc gaggccatcg
    11041 agcagagctc ggagtggctt aagacgaccc ttgcaggcga ttatgtccgc gagcacgaac
    11101 cgctcgggcg gatttacctg ccgacggctt cctatccgga aatgatgggt tgggcgctgc
    11161 cgccaaaagc gtccctggat tatgaggatc ttgtgcacag agtggagaag ctgcccgagg
    11221 cggccgcgct cgggcgcttc gttcacggag gggtatggca caattttttc tacaaatacg
    11281 aagaagccaa ccacatgcac aagcgcatgc tcgagttgag ccgccgctat gaagatctcg
    11341 acaaatcgac gccgccggga gttccggccc gcggcctgta tgaagagggc tacgagcacc
    11401 ttctgagcgc gcaatgcaac gatgcctatt ggcatggggt atttggagga ctttacgccc
    11461 cgcacttgcg cacgagcgtt taccgggctc tgctgcgagc ggaaaacatc gcggaaaaga
    11521 tcgacagctc gccgcgcaca acgcggcgct gggatttcaa cctcgatgga gcggatgaaa
    11581 tttttttggc gaatgagcgc ctggccctca tcgtcgcggc cggcgacggc gggaccgcag
    11641 ccgagatcga tttccggcca cgggcgttca acgcgatcaa ctcgctgcgc cggcggcccg
    11701 aggcgtacca cgccagactc caggacgcgc aaagaggcga agccggaggc gccgtctcga
    11761 tccatgacag gatggcggcg aaggaatcgg ggctggaatg ctatcttcaa tacgatcgat
    11821 acaaccggca cagtttccgc tcgctggtat tcgcggcggg acgcggattg gaagactacc
    11881 gcgcgggccg gctcgacgaa tctcccgagc tggctggagg aaggttttcc gttttgcagt
    11941 tggcacgcga ccgctgtgtg ctggcaggcc gtgacagaca acggctttgc cgcgtgcgca
    12001 ccgaaatctc cctagccggg ccgaacgtgg ccgtaaattg gagcctgtca caggcaaacg
    12061 cagaagagct ggaggccggg ttggaactgg ttttgaactt tctggcgccg gacgcgcgcg
    12121 accgttattt tcttctgccg gccgggctcg gggacgcggg cgggccggac catccggaga
    12181 catgcctgga agcgatcgcc tctccatctt ttacgcggca aaaactgcac tgggcggggg
    12241 aagcggcggg aagttgcatt gctttgctgg atgagtggct ggatgtgaga gtgaggatca
    12301 acgtgactcc accggcgaaa tggtggatta cgccgatatt cacggtttcc caatcggagg
    12361 gaggttttga gaaggtgtac cagggatcgt gtcttttgcc tcattggcgc gtacaggacg
    12421 gtgaactaca ggccacggtg cgcctggaat tcggcgcaac cggtgaacgc tcgtgaccaa
    12481 ggcggaacag ctcgctcaat tcgtcgtatc cgccggctac gatcgcctat ccgaggcggc
    12541 tcgcgaacag ctcaaaatcc ggattttgga cgccgtgggg tgcgccatcg gggctctggg
    12601 cgccgagccc atccggcgag tgcggcaaca catcgaggac ttcgggggtg gtgtccagtc
    12661 gggcttgatc ggcggaggcc gtacggcgcc ggatcgagcg gcattttaca acagctctct
    12721 cgtgcgttat ctcgacttta acgacagtta cctggccaaa ggggaaacct gccatcccag
    12781 cgataacctg ggagccgttc tggcggcggc agaatatgcc ggcgcaggag gccgggaaat
    12841 cctgaccgcg ctcgcggtcg cgtatcaggt gcaatgccgc ctgagcgatg tggccccggt
    12901 tcgcgcgaaa ggcttcgatc acaccacgca ggggtcgtat gcggcggcgg cgggggtcgc
    12961 gaaagcgttg gggctcgacg cgcaaagcac gatgaacgcc atcgccatca gcggaaccgc
    13021 attcaacgcg ctgcgagtga cgcgcaccgg cgcgctttcc aactggaaag ggctggccta
    13081 tcccaacacg gctttctgct gcacgcacgc cgcgtttctg gcgatgcgcg gcatcaccgg
    13141 accggccgag gtgttcgagg gtaacaaggg cttcatggac gcgattgcgg gccggtttga
    13201 gatcgactgg tccaaggaag acctggaaag ggtccgccga acgattgtca aaaaatacaa
    13261 tgcggagatt cattcgcaat ccgccatcga ggcggctctg gagctgaagc aggaacaccg
    13321 tttgacaggg cgggagatcc agcggatcga aattgagacc tttgatgtcg catacaacat
    13381 catcggaggc ggggaagaag gagacaaaat acaggtccgc acgaaggaag aggccgatca
    13441 cagtcttccg tacattgtgg cggcggcgct gctggacggg caggttatgc cggatcaata
    13501 caccccggag cgcattggaa gagcagatgt ccaaacgctg ctgcgaaaga tccgtgtgag
    13561 accacgggag gcctacagcc agagatttcc tgaagagatg ccgtgccgga tccagttgat
    13621 cctcgaaaac ggcgaaactc tcgtcaagga aaaaagggac tacgagggct tccacacaag
    13681 acctgcgcgg tgggaggccg tgttggagaa atttgagcgt ttgagccagc ggcacgcggc
    13741 gccagcgctg cggagcggaa tcgctgacca aatcgcaaat ctggaatcgg cccgcacggc
    13801 gggagaactg atggccctgc tggctcaagt ctcctgagga tgaacgagac cctgcgggcc
    13861 gcccggcgca ggcgcggccc ggggaaaagg gagagccctt gttacttgtg ctcaagccgg
    13921 ctgcaagccg tatcagccgc gctgggatcg atcgcagttt gcccttcctc gatatgctgg
    13981 atctttccct ctttatcaac cacaaacgtc gcgcgcgtcg caaagccgcg ctccggattg
    14041 aacacgccgt attcggtcgc gaccttgcgg ttgacgaaat cgcttaacaa ggggaacccg
    14101 actccgagtt cctgagccca atgctgaaga gtgggaatgc tatcaacgct gattccgagg
    14161 acctgggtgt tatttgcttc gaatttggcg agaccagcct ggtacgcctt catttccttc
    14221 gttcaaccgc cggtgaacgc gaggacaaag aacgcaagga cgacgttctt cttcccgcgg
    14281 taatcagcca aatggatcgg cttgcccgtc gtgctgggca gagtaaaatc gggcgccata
    14341 tcgcccacct taagatgggt tgccgccgcg gctgaaccta gactcatggc gagcaaacag
    14401 ctaccggcaa acagtcggct tgaaatcttc atggttcctc ccccgcccta ttttacgtcc
    14461 ttgtttgtgt gtttgcgcat aatttcactt gacggcgttt tcctgatggg ggatcacatt
    14521 gaggctttcc cactcagcca attgcttccg gatctgttcc gccgcggggg aatcaggcga
    14581 aagttggagg aaactgcgga attcgcccgc agcagagggg aaatttccct cgcgcgcaag
    14641 gatcgaaccg agcaaatgat gcaaggccgg attgcggccg gcggcttcgc ctttgagcgc
    14701 gttgcgagcc gatctttcag cgagttccat tttgcccaag ttgtaattgg ctatggcgtt
    14761 gatatagtaa gcctgcgtgt agtaagggtt catctgagag aggcggctgg ttatgtcggc
    14821 ggcggaatcc caccggctgg ccctcatttc aagggatgcc agttgcatgt aaggccgcgc
    14881 gtatttggag tccgcagcca aagattgctc gaaggctttg cgggcgccct cctcgtcttt
    14941 caaagccaga cggctttcac ccagcatgca ccaagcggcg gcaaacttcg ggtaaacctg
    15001 gacggccttt tccagctctt tggcggcctc ggccggcttt gggtttttct gctgcaacag
    15061 cttctgcgct ttttcccacg cttttttagc gtctttggga gcttccaggg tagtaatgct
    15121 gatcgctgta gcttgcacat tggccagagg atgcaagatg atagttccga catccggatt
    15181 atcaaagatg ctgcgccggc tcagcatgat ggaatcggag cgatagcccg ggagagaggc
    15241 gcgcaattca caacccatga ggttcacgac gcccgggttg gagctgtcca gaggcccgaa
    15301 ggctccgttc gcgctgttgc gatcccggcc atagcggccg cgggccggtc ccacatcgac
    15361 atcgcccacg ctggcatcca tcatggtttc agtttgctgc ccgagttgga aactgaagcg
    15421 gcccttgctg tcagtgtaag cttccggcct cggccggccg ttgcagactc gttcgatggt
    15481 caccggctca ggcggcggca ggccgccctc gatcatcact tttccggaga aaaagattgg
    15541 gcgctgctcg gagggaaagc gatcctgctg gctcggctgg ccgatgccgg gctggccgcg
    15601 gccgggcgaa ggaagcggag cggtaggtcc ggcgggcgcc gtagaagaag ctccgccctg
    15661 ctgtccggcg gggggtcgct gcgcccaagc gacgcaaaga ctcaaagcga ggaagcttac
    15721 cgaagactga agcacgattc gcatggccgg ccgcctccct taacagatat tccgatgcgt
    15781 atgacccgaa tgtacccaaa gcataacaca ggcatggagc gccggccagc aaacacaggc
    15841 ccaacggtaa gaatttaccg ggagagggca ggattgcctt tttggttggg cggataattg
    15901 accgtcagcc agttcgtgat caagcggaac gccgttttgg gtgtatcagc gtactgaatc
    15961 cactggcgat cctgctcgtc gatcatgccc catttctgca tggcatcgaa gcttaggacc
    16021 tcgtcccaat atttgcggcc ataaatgagc acgagcattt tcttcttgaa cttgcctgtc
    16081 tggttcaggg tgaggatctc catcagctcg tcgcacgtcc cgtagccccc ggggaagatg
    16141 atgagcgcct tggccaggta cgcaaaccag tacttgcgca tgaagaaata gtgaaaatcg
    16201 aagctcaacc ggcgcgtgat gtagggattg ggcagttgtt cgaacggcaa actgatattt
    16261 aggccgatgg agcggcctcc ggcatcggcc gcgccgcggt tggcggcctc catgatgcct
    16321 ggaccgccgc cagaacacac gacaaagcgc gcgggttcgg aggagagggc catcgaccac
    16381 tcggtcagca tacgagccag ttcccgggcg gcctcgtagt aggcgcttgt tttgagaaga
    16441 cgctgggctt gctttcgagt cagagcggag cgagccttgc ggatgcactg caaggcggtt
    16501 tcgcggtcgc atacacgggc cgagccgaaa aagacgatcg tgtcgtagac tttttcttcg
    16561 cgaaagcgag cgaagggctc caaatattcg gcgagaatgc gcagaggacg agcgtgatag
    16621 ctgttgagaa aggcgatatt ccggtaagct ttcggaggaa tgtaagtcat agaacgggat
    16681 caatccttaa ccaacgttag aatcattcca gtatcggtag ccacccaggc atgagtggaa
    16741 tccggggcgg cgagcaaggc agcccgggct tcggcgcggt aatcgacctg catttcagtc
    16801 cagttgttga agccgacgga gcgaaggatt ttcagtttag agggaatggc cggaaagggc
    16861 gcccggccct cctgatcgac ggcggccgcc agggttgtat cggggcccag gagggccaaa
    16921 tcggtaagcc agcggctcct attttgataa accggatggc tctgaagagt ctttaggtcc
    16981 agctcgacaa tagcggaagg agaagccagc gaatccgggc gctggatcaa caggagggcg
    17041 gcggcgccgg aaggcaagat gcgcgcccgc acaatttcgc caaaattccg caggaccgag
    17101 gggttccagc ttcggccccc atccgcggtg tggagaaaga ccgcagtagt ggggtggcga
    17161 gaagggggcc gcagttccgg ccggatccac tccaggatct cggaacgggg gtctggtgga
    17221 cggctccagc cggtcagcaa gccggtctcc tgctgaaaag cggcccaagt ataaaaagtg
    17281 ctggcatcgg gaggatcggg acgccgggaa gcttcgagga gctgccagga gcggcctccg
    17341 tcgtttgtgc tgtagatttg cttctgggag caaagcagcc agcctcggga ttcgtcgagg
    17401 aagaaaacgc ggagaacatc ggcgcgaacg cccttgggag cggcggattt ccaggtccgg
    17461 ccgccgtcct gagttttcca aaggtgattc ttggccccgg ccagccatcc ggtctggtca
    17521 ttgctgaaga agagagaaac gaccgcctct ttgagtgaaa cgagagtcca gttgcggccg
    17581 ccatcatagg tgagcgcaag aaccggccgc tggcgatcgc gcccggcaac gacgcccagg
    17641 gctgcgccgt gcagcggcga ggaaaacgag aggtcggtga aggcgagcga tgagtcgttc
    17701 ttatcgtaaa aatactgaag atcccagcga tcggcggccg cggcggaatt cagaaacagg
    17761 agagcggtaa ccagggttgc ccaaacgcca gtccaaccca taagacgcgt tagtttatca
    17821 tggaggggcg tcagccgaga ctggagtgag gagtatgagg ctcgctgcgg ttctgctatg
    17881 ggcagtagca ataggttccg gggcggcggc tgaactgccg gccggcgccg agattcaggt
    17941 gcggctgcaa accgccgccg gaagcagcaa atccaaagca ggagatgttg tgcgcgccac
    18001 tgtgattgta ccagtgctgg cgggcggcga cattctgatt ccagcgggga gcacaatcac
    18061 aggcaaggtg aagggagcgc agccggcgaa aggcgggacg gagcgagcgg cgctggaact
    18121 ggagttcggg gaactgttgc ggcccggcga ccagcaggca acgcaattac aaacgaagat
    18181 tgcggctgtg gacaatgcgc gggaaaccgt tgatggcagc ggcaggatcg tgggaattgc
    18241 ggcatcagag acgctttccg cacagatgga taaggggctt tccaaactgg agcaacgggc
    18301 ggcggggctg gcgggattcc tggaggcggc gaaagaagct gttctcaaac aggctgatgt
    18361 cgaaatcgac tatgagccgg gggtggaggt gaccctgcaa ctgaccagca gcgtaaagct
    18421 aaacgcggcc cagctcgcac cgggcaatgc tccacagccg attgaggatg cagcaggcct
    18481 ggcggaactg gtgaaccgtg agccgttcca gaccatggcg gaaaagccgc cgcggccgtc
    18541 ggacattatc aacttgatgt tcatcggggg gcgggaacag attgaagcag cattcgccgc
    18601 agccggctgg gcgacggcgg aggcgctgaa cgccagatca ggaatggaga cgttccgggc
    18661 gatcgccgag ggaaggggct accagcaggc gccggtatcg actctgctgc tggaagggcg
    18721 gaagccggac ctggtgtttc aaaagcagaa caacaccttt gcgcaaagac accacatgcg
    18781 cgtttggatg cggcgcgatc agtttcatgg ccaagccgtg tgggtggcgg cggcgacgca
    18841 tgacatcggc atcgagttct cgccggaaaa ccggacgttc atacacaaaa tcgattcgca
    18901 aatcgaccgc gagcgggcca aggtcgtcaa cgacttgctt gtcacgggca aagtacgcgg
    18961 gctggcgctg gtggaacggc caggagcgcc ccgggaaagc caaaacgcca caggcgacaa
    19021 gctggaaacc gacgggagca tggccgtgct ccagttctaa gcggtctcca ggcacatttc
    19081 cggagcaaag ccattcaggg ctttggggga aacgaggaca ggacaagatc cacgtgagac
    19141 gcgtcatgga cgcgttcgga agccatgcga gccaggacga gggcgcagcc tacgagttcg
    19201 cccacagtgg tgagcagctc catttcgctg ccggtgtgct ggtgaggcag gcgatgctgc
    19261 acattgatga cgcccacgac cttgtctcgc gaaatgacag gaacggaaag gaaggcttcg
    19321 aatgtatcct cgggaagcga gccgaaaaac ttgaaacgcg gatcgcggta gcactctttg
    19381 gaaatcgaaa gcagccgccg ctcccgcgcg acccagccgg taagaccttc gttcaagcgg
    19441 aggcgaatct tgccgatctc cgaaggatgc gggttattgg aggcgcacag gacgagctcc
    19501 tcgcgatcga gcagataaat caagcaggag tcgcactgca tgaattccgc gaccaggtcc
    19561 acaatcccct gcaagacctc ttgcagactg aggttgcgaa ccatgaaccg gctgattttc
    19621 tggaagagcc gcagttgctg ttccgtgcga gcgagccgcg cttcgaggtc cttttgtctc
    19681 tgtcccacga gcttcccgcg ccccagcggc gcaacacttt tattatagcc cggcaggtct
    19741 gaccggctga tccggcagga aagcagggcg caagcgccaa tataggcgat gcagagataa
    19801 aacggccgct accgagcgtt atactaagat gtgaatcttc cggcgaccgc tcgatcgctt
    19861 tctgaggttc attccagcat cgaagtaccg gatctttccc tttggaggcg aatgttcgcc
    19921 ttcgcgggcc cggcctatct ggtgagcgtt ggctatatgg acccgggcaa ctgggcgacc
    19981 gatctggagg gaggcgcgcg tttcggctac caactgctgt gggtgctggt aatgtcgaac
    20041 gcaatggcga tcctgctgca aacattaagc gcgcgcctcg ggatcgtatc cgggaaggac
    20101 ctggcgcaag cttgccgaga gcactacccg caagcagtgg gattcgccct gtgggcgatc
    20161 agcgaggtgg ccatcgccgc ctgcgatttg gcagaagtgc tgggcacggc gatcgggctt
    20221 cagcttttat tcggaatacc gctgatcacg ggcgtgttga tcacggcgct tgacgcactg
    20281 ctcgtcctgt ggctacagcg ttttggaatg agggtgctgg aagcgttcat tctatcctta
    20341 atcacggtta tcggcgtctg cttcctgatc gagatcgttc tagccaagcc ggggctgaaa
    20401 gagatggccg gagggctggt tccgcgtctc aacggggaaa gcctttacgt ggcgatctca
    20461 attctgggcg cgacggtgat gccgcacaat ctctatctgc actcggcgct ggtgcagacg
    20521 cggcggatcg attcgagcga gaaggccaaa caagcggcgt gccgatataa cctggtcgac
    20581 tcggtggttg cgctcaatgc tgcactgctt gtcaatgcgg cgattctggt gatgtccgcg
    20641 gcggtctttt tcagccgagg catagcggtg cgggaaatcc agcaggcgca caagctgctc
    20701 gaacctctat tggggacgac gttggccagc ggggcatttg ccatcgcgct catcgcatca
    20761 ggacaatcgt cgaccatcac gggaaccatg gccggacaga ttgtgatgga gggattcctg
    20821 agtattcgca tgcggccttg ggtccggcgg ctggtgaccc gggctctggc gatcacgccg
    20881 gcggcgctgg taatcgggtt cgcgggggaa ggcgcgacgc tgaaactgct gctgctgagc
    20941 caagtcattc tgagcttaca gcttcccttc gctgtgattc cgctgattca tttcacaagc
    21001 gaccggagcc ggatgggggg tttcgcaaac cggaactggg tgaagctgct ggcgtgggcg
    21061 gcggcggcaa tcatcgtggg attaaacgtg caactggttt ggaccacgct agcgggatgg
    21121 gtttcgacat ccgggcgctg gtctccggcc attgaatttg gcgtgattcc cgcagtcgcc
    21181 gctttgggac ttttactggt ttgggtaacg gccgaaccat ggatcagacg gcaagcgccg
    21241 cggcgggaag cggtcactat gccggcgatc gaggcaggag cgagggagcc ggcctaccag
    21301 agaattctag tcaccctcga ccactcggcc ctggaccgcc aggcgatcgc gcacgccgca
    21361 gcgatcgcgc gcacgggagc tgcgcgaggc gcccgcatct atttggtgca cgtggaggaa
    21421 ggcgctccca gccggctata cgggaacctg gcgtcagacg cggaggtgag agccggtgaa
    21481 gattatctgg aaagcgtgcg gcgaacgctg gaggcccaag gcattccgac cgaagggttc
    21541 attgcgcact cgagcaatcc cggggaggaa atcgtgcggg tggcccggga agtcggggcg
    21601 gatctgctgg tgatgggcgc acatggccac cgcaagctga aagattttct gctgggagat
    21661 acgattgagc cagtgcgcca cgcgctggac acacctatcc tgatcgtccg acagagcccg
    21721 aagtgaggca gtttcgcggt tcacggcgcc acgttgctgc cacagatttc cggtgggcca
    21781 cgcgctttgt cgcttgtccg aaaaccggtc ccgattcaat tcagagacct cttcctggag
    21841 acgcgcatga acactgttga gttcaccggg ctggtatttc tggtggctgg agtggcggga
    21901 tttctggggt ctctgacggg tctaggagga ggtgtggtca ttacacctgt gctgacgctc
    21961 ctattcggcg tggacattcg atacgcgatt ggagcgacgt tggtttcggt gatcgcaacg
    22021 tcctccggag cggcctcagc gtatgtgcag gagggtttct cgaatatccg gatagcgatg
    22081 tttctggaga ttgctacggc cggaggcgcg atatgcggag cgctgctggc cgcgaagacg
    22141 ccgacgtccg cattggcggt gattttaggc gccgtcttgc tgtattcagc gtaccgctcc
    22201 gcgcgcgcgc attcggacga ggtttcggcc caagcgccgg acgctttggc ggtgcgcttg
    22261 aaactcgatt cgagctatcc gacgccggca ggtagacagc attatcatgt ccgcggggtg
    22321 aagagcggct tcggcataat ggcgcttgcg ggcatcattt ccgggctgct tggcattgga
    22381 tccggcgcgc tgaaggtgat cgccatggat caatttatgc ggctgccatt caaagtatcg
    22441 acgaccacga gcaatttcat gatcggggtg acggcggcgg ccagcgcggg cgtctactta
    22501 agacggggat atatcgaccc ggcgctggcg atgccggtga tgctaggagt gctcgcgggc
    22561 tcggtgttag gcgcccgcga attgacgcag accaagagcc ggatcctacg agtggtattc
    22621 gggctggtag tggcggctct ggccgtagaa atgatttacg gcggggcgac ggggaggatt
    22681 tgatcaatga tccacggtcc gagcgaggag cgcgtgcagc aggtcatcgg tatgcttttg
    22741 cgcctcgggg taatgatcgc ggcgggagtc gttgcggcgg gcgggatgtg ctacctggtt
    22801 gagtccggag ccgcccggcc tgagtatcat gagttccacg gaatagcaga ggatttaaga
    22861 acggtaccag gggtctggcg gggggttctc gatcttcgct gcaagagtat tatccagcta
    22921 ggaattttga tgctaattgc aactccgatc gcgcgagtcg cattttcagc ggcggcgttt
    22981 gggcttcaac gtgattggac gtatacggcg attacgtcca ttgtgctgac gatcctactg
    23041 tacggtctgg tttggggcgg ttagtttgat caatttgcga ggagccgtca ggaggcaagg
    23101 gtaggggctg ctccggcggc agcaccgcgc gccgagcgcc ccgcgatcag acgcagggcc
    23161 atctcaatcg cacagatcat gttcgtagga tcggcaaggt tcttcccggc gatatcaaag
    23221 gccgtgccgt gatcgaccga ggtgcggata atgggcagac cgagcgtaac attcacagtt
    23281 tcttgaaaag cgatgagctt ggaagggatg tggccctggt cgtgatacat agcgacaacc
    23341 atcgcaaact cgcccattac ggcgcgatga aagacggtgt ccggcgagat ggggccggcg
    23401 acattccagc cacgtgcgac ggcttgctcc acggccggaa cgatctgctc gcgatcttca
    23461 acgccgaaca ggccattttc gccgcagtgg ggattgaggc cggccacagc gattcggccg
    23521 ccggccaagc cgagttgttg cagcccgaga acggccagct cgatggttct gaagacgcga
    23581 tccgtggagg c
//
LOCUS       DASREK010000257        12508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_792830, whole genome shotgun sequence.
ACCESSION   DASREK010000257 DASREK010000000
VERSION     DASREK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12508
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792830"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1403)
                     /locus_tag="VFA54_13925"
     CDS             complement(3..1403)
                     /locus_tag="VFA54_13925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS51960.1"
                     /translation="MTSEEIAAVVEEIRQRVRARYQKQVDGVPDFCVPSLIAAAQARD
                     AALGKVASIGTVNPRPPGLVNSIIQGIKRAVSRALAWHVREQVEFNRAVIEYMERSLA
                     VLEEHNRNLLQLAQLARDALDAARNVSEWRTGWVEKATKSEIQMLRSIAELQGAFQHR
                     VSLLESNFREHIKSQHADYLRALERTTLDVQRRLWDDLAKIRQEYERQREMQTELRLI
                     RQRMSAWGPSAAQNIAVSASPPAAAENGTANLADLDYARFAERFRGSAAHVAESQIFY
                     VPLFQGRRRVLDLGCGRGEFLEIMRQNGIGAAGVDSDAESVLLCREKGLDVIQADLFA
                     HLSSLPDESLDGVFCAQVVEHIPPLRLPGLVKLISQKLERGGVAVIETPNPECLAIFA
                     TNFYLDPTHVRPVPAALLHFYFEESGLGAIEIHQRAPAAEQFPEIAALAEVTGLAGFQ
                     KRFLGGLDYAIVGRKL"
     gene            1497..2120
                     /locus_tag="VFA54_13930"
     CDS             1497..2120
                     /locus_tag="VFA54_13930"
                     /inference="COORDINATES: protein motif:HMM:NF023540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metalloprotease CJM1_0395 family
                     protein"
                     /protein_id="HZS51961.1"
                     /translation="MMTFLVAGSASLAAGTLPSAQQPGSSPERDSSVQNIMQAGAKQP
                     GKLQKQNASLHETQFSQQIALQQLAQIDRRVHAHEQAHLAAAGAYARGGPSYTYTTGP
                     DGKLYAVGGEVDLDVSPVPGNLPATIQKAIAVQAAAEAPEDPSPQDRRVAAAAAQMIV
                     DAQLELTVQRARQARNAGSRHGNPKAYSPPPDDTGLRNAGNQLDLVV"
     gene            complement(2130..2849)
                     /gene="pgl"
                     /locus_tag="VFA54_13935"
     CDS             complement(2130..2849)
                     /gene="pgl"
                     /locus_tag="VFA54_13935"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HZS51962.1"
                     /translation="MRQAPTTEAAASDAAQFILQILRAAVEKRGSASIALSGGSSPKA
                     LFSRLAAAKFDWNGVHFFWVDERAVPPGDADSNYRLARELLLEPARIPHRQIHRIQAE
                     LEPEEAARRYLAEMRSFFGLSAADLPHFDVVHLGVGPDGHTASLFPDGPLLADRSGIA
                     AAVYVEKLKSWRITLLPGVLLAAGQIVFFAPGEEKREILQRIWGPEYNPARWPAQLID
                     REGKNVAWFADRAAAEGIADC"
     gene            complement(2864..3958)
                     /locus_tag="VFA54_13940"
     CDS             complement(2864..3958)
                     /locus_tag="VFA54_13940"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HZS51963.1"
                     /translation="MTIDPEELRARLDQVWAALARDAVADGHEVMRACGRTLLSVVDQ
                     SRESDDVLEMLAELLHANPSRSIVVLLAAGDTEQLAADVDARCWMPSGSRQICSEQIV
                     IRCSERTLAEVPGVVTPLVAADLPVVLWCASERARRSPAFPSLAAPARRIILDTFSAE
                     LPLEALETLIEQQRRSPALIADLSWTRLTRWRALLAQVFENRLYRAHIPDFNRITIQY
                     QDQSGRIPPTVLLFAGWVTSRLRWQPQKTQPAAESRKILSFQRPNGPALVTLEHQDSL
                     DSPESLLSFGLGGEAAAAPRISIGRVSEACGEVRIEAAGLDPVMNRVSLPAPTDLMLL
                     GEELAISQRDPAFEESLERAVQIARNLQAG"
     gene            complement(3955..5499)
                     /gene="zwf"
                     /locus_tag="VFA54_13945"
     CDS             complement(3955..5499)
                     /gene="zwf"
                     /locus_tag="VFA54_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZS51964.1"
                     /translation="MSSPAAALDANPFLDPLRFRKGASDCVMVIFGASGDLTRRKLLP
                     ALYNLARDRRLSAGFAVAGVARRPIGDEDFRAKMKAAVSEFSDSGPLDESVWDGFSQG
                     LYYVQGDFDDPAAYTRLAERLRQISQARHGSGNVLFYLSTQPSYYASIVKNLGASGLA
                     GPDHSGWRRIVIEKPFGHDLASARQLNGVIHSVFDENEVYRIDHYLGKETVQNILAFR
                     FANGIFEPLWNRRYIRQVQITAAESIGVEGRGAFYEEVGALRDMVQTHLLQVLATVAM
                     EPPARFDASSSRDEKAKVLGSIRPLTHDEVLIEAVAGQYGPSRVGGRGLPGYRQEPGV
                     RPDSQTETYAAVTFFIDNWRWAGVPFYLRTGKRMPKRVTEIVIQYSPAPHRLFPDSDA
                     ATEPNVLVLRIQPEEGISLKFHSKEPGAGMRLRGVSMDFNYGSSFGGRSPSAYETLLL
                     DAIDGDPTLYTRQDMVEASWAAVQPLLDVWKNENHDFPNYPAGTWGPAEADRMLERRG
                     HRWRNG"
     gene            complement(5591..6133)
                     /locus_tag="VFA54_13950"
     CDS             complement(5591..6133)
                     /locus_tag="VFA54_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51965.1"
                     /translation="MKPPVRPLLFFFFLLPFPVRAVDCACNHADPATLDQRSCSLCRE
                     AEKQSSGVFFLKDANPRKPNRTLALPLQHGKGLQRLGDLSPQARADFWKAAIAKAEQL
                     WPGGWGVAYNADTTRTQCHLHVHIGKLIDGVDESTGRLFSRPEEFPLPDAGVGLWVHP
                     VSGGFHVHMDRETAEFVLVR"
     gene            complement(6130..7377)
                     /locus_tag="VFA54_13955"
     CDS             complement(6130..7377)
                     /locus_tag="VFA54_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51966.1"
                     /translation="MADPAVAVAAVPARSMRWPVVGLLTVGVVIAYIDRINLSTALPA
                     VRQSLSLTAESAGVVLSAFFWFYAALQVPAGWVVDRFGVKWPYTAAFLFWSLVSASTA
                     LVATFTGLLAVRLLLGIGESIVTPASLRYIGLHFPEKQRGLALGIYFSGTKYGPAIGA
                     PLAAYLVRDYGWQRMFLLTGAGCLLWLIPWVLLVQNDAGGNSSSKHSEASVPSFSWRV
                     MASSPVMWGTFLATFCYMYFVYFCMTWMPTYFKDRHGLSLTNSGWYTFMSFGGMATIA
                     IAGGFLADRLIARGWNAVKVRKTFIIVGFLLAFTEIIGAVADSAPATLFFAVFSLSGL
                     GLATANYWALTQTLVPARALGRVAGIQNTAASLAGIAAPWITGWLVEQTGKFNAPLAA
                     VGFWLVLGIGCYLFLVREKYALR"
     gene            7523..9076
                     /locus_tag="VFA54_13960"
     CDS             7523..9076
                     /locus_tag="VFA54_13960"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS51967.1"
                     /translation="MACRTAGHPAGRTARLLAVGACLLVWPPASAQNSSVVEGASSGA
                     AAYRLIDEVANTPTLESNLRVLCDEIGGRLPGSDAMRRAISWAIDAFHKAGLTSVHTE
                     SFSVPNSWAEGATRAEVISPVRFVIHAAATGWSSPIPNGIEAEVLSGQRGEEGEVERL
                     GAAGKGKIIIIGSEPLNNFQDLGMEQRRATIAMREAEKIGAAAVLFMSTRPQGLLYRQ
                     ANLVDGRLDKLPSAVVAREDAQRLLRLLEAGRRVRMRLALPNKIGGPFQAKNVVAEIR
                     GAEKPAEVVIVGAHLDSWDLGTGCLDNGCNAALVIEAARAIQAAHLRPKRTIRFILFD
                     GEEEGLLGSRAYVAQHRAELDNIVAVVIHDMGSGRISGYSLGGRGEIEPALDEIMGPV
                     NYLGATNHTNDAFFGTDHFDFLLQGIPTLVANQDTTVYVPNYHAQSDDFDKVDLKELK
                     DQATIAAVTAYNIADRPDRLGKRLTRAQIARLLRETRLDEQLKFLGLWQEWESGRRGR
                     RDGGDSSAR"
     gene            complement(9077..10054)
                     /locus_tag="VFA54_13965"
     CDS             complement(9077..10054)
                     /locus_tag="VFA54_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS51968.1"
                     /translation="MLFPAPEPLVGIARLAASSPCTEAKSQVAYYELECRSILNKCSS
                     PRMPFPYTVNPYRGCEFGCRYCYARYTHEFMGMEDANLFEQKIYVKAHAAGILRRELR
                     RLPAGEIAMGTSTDPYQPAERRYGVTRSILEVFAGERGWTLSITTKSNLIARDIDLLV
                     SIARSNVLHVNMTVTTTDAALARKLEPRAPRPDLRLAAVRLLASAGISVGVFLNPILP
                     GLTDAVTNLDAVAAAAAEAGAQYLGGGILFLMPSAQKVFFPFLEAEFPELAARYRARF
                     AHSPYIRGEYANMIRERVARIRSRHGLASSPRQYLPAIAPEDPQLPLFE"
     gene            10149..10994
                     /locus_tag="VFA54_13970"
     CDS             10149..10994
                     /locus_tag="VFA54_13970"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HZS51969.1"
                     /translation="MKRFLRLLREAAVSAFYNKCFDTAKSAAYSSILSFFPALMVTAA
                     FLSGHVSAVVDEISIALGRVLPPDAYEVAEQYLKVQGARAHGLLAGAWFVAIWSASNV
                     ISCLIQGFQTAYKVRVNRSLIKSRLVALALLPISGVPLLLATLLLFFGEQVANWLAVH
                     FIEARWWLAPAGMAVRWSVAFATSVFVIGLLYHVGSGRRQQWRLVWPGAVLATGAWLG
                     ATLGFAWYVRKMARYGELYGSISTAVVLLIWMYIVNLAVIYGCEFNAAYERELSAAEA
                     PASSG"
     gene            complement(11057..>12508)
                     /locus_tag="VFA54_13975"
     CDS             complement(11057..>12508)
                     /locus_tag="VFA54_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HZS51970.1"
                     /translation="SVGPQSGTVTFTLTSAGSASPVTTTLTLAVSGQGQLPVSITVTP
                     SAIDFGNVAVGSSATASVAVSNNGGVQASVTGSATGPFSLSATTFNLAPNANQTVTIT
                     FAPAGLGPAQGSATFAAGNVTQTVTLKGTGVAPSFAFLFGQSNPSATAVQAGGTVPLP
                     PVAVGSNSTTQFFIKNTGANPAAINSISVSAGSFALLGLPAMPANVLSGDSLAFTIRF
                     TPAAPGGASASLTVNGQTFNLTGTGLVSGVTITSGVFSAAQQPNITISLAQSYAAPIS
                     GQLVISFSPNAAGAPDDPMIQFATGGTCSNFNGPAGRCVAFTIPAGATAAQFPGNVAQ
                     IGLQTGTVAGSINLTATFSAGGTDVTPSPAPVGALTIARSAPVIQSVTATRSGSTLQL
                     VIIAFATSREVNTVRVQFNPAAGANLQTTALSVDVGSIFTNWFASSAAASFGSLFKLT
                     LPFTVQGDPGAVTGASVTLLNGDGQSAASQTNF"
BASE COUNT         2554 a         3814 c         3855 g         2285 t
ORIGIN      
        1 atttagagct tcctgccgac gatggcgtaa tccagcccgc cgaggaatcg cttctgaaat
       61 ccggccagtc ccgtaacttc ggccagcgcg gcgatctcgg gaaactgttc ggcggccggg
      121 gcgcgctggt gtatctcaat cgccccaaga ccgctctctt cgaaatagaa gtgcaacaaa
      181 gcggcgggca ccgggcgcac gtgtgtgggg tcgagataaa aattggtggc gaaaatggcg
      241 aggcactcgg ggtttggcgt ttcgatgacc gcgacgccgc ctcgttcgag tttttgagaa
      301 atcagtttta ccaagcccgg cagccggaga ggcggaatgt gctccactac ctgagcgcag
      361 aagacgccat ccaggctttc atcgggcaaa gaagaaaggt gcgcaaacaa atcagcttga
      421 atgacatcga ggcctttttc gcggcaaagc aagaccgatt cggcgtcgct gtcgacgccg
      481 gccgctccga tgccgttttg ccgcatgatc tccagaaact ccccgcggcc gcagcccaga
      541 tcgagcaccc tccgccgccc ctggaataag gggacgtaaa atatttggct ctccgccaca
      601 tgcgccgcac tcccgcggaa gcgctcggcg aatcgggcat agtctaggtc cgcgagattc
      661 gcagttccgt tttctgccgc ggcaggcggg ctggcggaaa cagcgatatt ctgcgccgcc
      721 gagggacccc aggcgctcat tcgctgccgg atcaggcgca gctccgtctg catctcccgc
      781 tggcgctcgt attcttgccg gatttttgcc aaatcgtccc aaagccttct ctgcacgtcg
      841 agggtggtcc gttcaagtgc ccgcaggtaa tcggcgtgct gggatttgat gtgctcgcgg
      901 aaattcgact cgagcaagct cacgcgatgc tggaatgcgc cttgcaattc ggcgatcgac
      961 cgcagcattt ggatttcgct tttggttgct ttttccaccc atccggttcg ccactcgctc
     1021 acatttcggg ctgcatcaag cgcatcccgg gccaactggg ctagttgcaa aaggttgcgg
     1081 ttgtgctcct ccaaaacagc cagcgagcgc tccatatact cgatgacggc cctgttgaac
     1141 tcaacttgtt cccgcacgtg ccatgctaaa gcgcggctca ccgctctctt aattccttga
     1201 ataatggagt tcactagccc cggaggacgg ggattgacag tgccgatcga agcgaccttc
     1261 cccagggcag cgtcgcgagc ttgcgcagct gcgatcagcg aaggcacgca gaaatccggc
     1321 actccatcaa cctgtttctg atagcgggcc cggacgcgct ggcggatctc ctctacgacc
     1381 gccgcaattt cctctgacgt cataaaagcg agggaacgtg ccttaaaaat tgtagcagac
     1441 gcattttttg ttcaactccc gggccggacg gccgatacga ggagagggat cagtgtatga
     1501 tgacgtttct cgtagccggg tcggcgagcc ttgctgctgg gactctgccg tctgcgcagc
     1561 agcctgggtc cagtcccgaa cgggattcat ccgtccaaaa cattatgcag gccggcgcca
     1621 agcaacctgg caaactccag aaacaaaacg cctccttgca cgaaacacag ttttcccagc
     1681 aaatcgcgct ccagcaattg gcccagatcg accgccgcgt gcatgcccac gagcaagcgc
     1741 atctggccgc cgccggcgct tatgcccgag gaggacccag ctatacatac acgaccggcc
     1801 cggatggaaa gctttatgcc gtaggcggcg aggttgacct cgatgtttca ccggttcccg
     1861 gcaacctgcc ggcgacgata cagaaagcga tcgcggtgca ggcggcggcc gaagccccgg
     1921 aggacccatc cccgcaggac cgccgggttg ccgcggcagc ggctcaaatg attgtggacg
     1981 ctcaactcga gttaaccgtc cagcgggccc ggcaagcgag gaacgccggc tccagacacg
     2041 gtaaccccaa ggcgtactcg cctccgccag acgataccgg tctgcggaac gccggcaatc
     2101 aacttgacct cgtggtttga gacctgccct caacaatccg cgatgccctc ggcggcggct
     2161 cgatcggcga accacgccac atttttcccc tctcgatcga tcaattgcgc cggccacctc
     2221 gcggggttgt actcggggcc ccaaatacgc tggagaattt cgcgtttctc ttctcccgga
     2281 gcaaaaaaca caatttggcc tgctgcgaga agcaccccag gcagcagcgt aatgcgccag
     2341 ctcttcagtt tttccacgta aacagccgcg gcgatccccg agcggtctgc aagcagtggc
     2401 ccgtcaggaa acaggctggc tgtgtggccg tcagggccca cacccaggtg cacgacgtca
     2461 aaatgcggca ggtccgccgc agagagcccg aagaagcttc gcatctccgc caggtagcgt
     2521 cgtgccgcct cctccggctc cagctccgct tggatccgat gaatctgacg gtgtggaata
     2581 cgtgccggct cgagcagcaa ttcgcgcgcg agacggtaat tgctgtcggc gtcgccaggc
     2641 ggcacggcgc gttcgtccac ccaaaagaag tgaacgccat tccaatcgaa tttcgcagcc
     2701 gctagccgcg agaaaagagc tttcggcgag gaaccgccgg acagggcgat tgaggccgat
     2761 cctcgttttt cgaccgccgc ccgtaaaatt tggaggatga actgggcggc atcggaagcg
     2821 gcggcctcgg tggttggagc ttggcgcacc cgggcagaca tcatcaaccg gcctgcaagt
     2881 ttcgggcgat ctgcacggcc cgctccaggc tctcctcaaa cgccggatca cgctgggaga
     2941 tcgccagttc ttctccgagc agcatcaagt ctgtcggggc aggcagggac acgcgattca
     3001 tgactggatc gaggcctgcg gcctcgatcc gaacctcgcc gcatgcctcg ctcacgcgcc
     3061 cgatggagat tcggggcgca gcggcggctt cgccacccag cccgaaggag agcaggcttt
     3121 ccggggagtc cagggaatcc tggtgttcca aagtcacgag agccggtccg tttggccgct
     3181 ggaaggagag gatctttctg ctctcggcgg caggctgcgt tttctgcggc tgccagcgca
     3241 gccgtgacgt cacccagccg gcgaaaagca ataccgtcgg cggaatccgg cctgattggt
     3301 cttggtattg gattgttatg cggttgaaat cgggaatgtg tgcgcgatac agcctgtttt
     3361 cgaaaacctg agccagcaac gcgcgccagc gggtcaagcg ggtccagctc aaatcggcaa
     3421 tcagggccgg acttcgccgc tgctgttcga tcagcgtctc caaggcctcg agcgggagtt
     3481 cggcggaaaa cgtatcgaga ataatccgcc gcgcgggcgc cgccaggctt ggaaaagccg
     3541 gcgagcggcg ggcgcgttcg ctggcgcacc agagcaccac aggcagatcg gcagctacca
     3601 gcggcgtcac tacgcccggc acttcggcca atgtccgttc cgagcagcga atcacaatct
     3661 gttcgctgca aatctgccgg cttcccgaag gcatccagca gcgggcgtct acatccgcag
     3721 caagctgttc tgtatcaccg gcggccagca ggacgacaat gctgcggctt ggattggcat
     3781 gcagcaactc ggcgagcatc tccagcacat cgtccgattc ccggctctga tcgacaaccg
     3841 agagcagcgt gcggccgcag gcgcgcatga cttcatggcc gtctgccacc gcatcgcgag
     3901 ccagggcggc ccagacctgg tcgagcctcg cccgcaattc ttcgggatcg atggtcatcc
     3961 gttccgccag cggtgcccgc gtctttccag catgcggtca gcctccgcgg gcccccatgt
     4021 ccccgccgga tagttcggaa aatcgtgatt ctcgtttttc cacacgtcca gcaacggctg
     4081 aacagccgcc caagaagctt ccaccatatc ctggcgggta taaagcgtcg ggtcgccgtc
     4141 gatggcatcc agcaggagcg tttcgtaggc cgagggagat cggccgccaa aacttgagcc
     4201 gtagttgaag tccatcgaga cgccccgaag gcgcatgccc gcgccaggct ccttggaatg
     4261 aaattttaag gaaatgcctt cttccggctg gattcgcagc accaaaacgt tcggctcggt
     4321 agcggcgtcc gaatcgggga acaagcggtg cggcgccgga gagtactgaa tcacgatttc
     4381 ggtcactcgc tttggcatgc gcttgcccgt gcggagatag aagggcactc ccgcccagcg
     4441 ccaattgtcg ataaaaaaag tgaccgcggc gtaggtctcc gtctgcgaat ccggccgcac
     4501 tcccggctcc tgccggtaac ccggaaggcc gcggcctccc acgcgggaag gtccatattg
     4561 gccagcgaca gcttcgatca ggacctcatc gtgggtcaac gggcggatcg accccagcac
     4621 cttagccttc tcgtcacgag acgaagatgc gtcgaaacgc gccggcggct ccatagccac
     4681 cgtcgccagg acctggagca agtgggtctg caccatgtcg cgcagagcgc cgacctcttc
     4741 gtaaaaggcg ccccgcccct ccacgccgat cgattcggcg gcggttatct gtacttgccg
     4801 gatgtagcgg cggttccaaa gcggctcgaa gatgccgttg gcaaagcgga aagccaatat
     4861 gttctgaacc gtctccttgc cgaggtagtg atcgatgcga taaacctcgt tctcgtcaaa
     4921 aacgctatga atcacgccgt tgagctggcg ggcgctggcc aaatcatggc cgaaaggctt
     4981 ttcaataacg atgcgccgcc agcctgaatg atcaggccct gccaggcccg atgcacccag
     5041 atttttaacg atcgacgcgt aataactcgg ctgagtcgag agatagaaca gaacgttgcc
     5101 cgagccatgg cgggcttggg aaatctggcg caggcgctcc gcaaggcgcg tataggccgc
     5161 cggatcgtcg aagtcgcctt gcacataata gaggccctgc gagaaaccat cccataccga
     5221 ttcatcgagc ggtccggagt cggagaattc cgacacggcc gccttcatct tcgctcgaaa
     5281 atcttcatca ccgatcggcc ttcgcgcgac gcccgcgacg gcgaaaccgg cggacaagcg
     5341 gcggtcgcga gcaaggttgt acagtgcggg cagcaatttc cgccgggtca agtcgccgct
     5401 ggcgccgaaa atcaccatta cacagtccga agctcccttt ctgaaccgga gagggtcgag
     5461 gaaggggttc gcgtcgagcg ccgcggccgg cgagctcatg aggcgagtct actataagcg
     5521 aaggtttgta cgcttgcggc ccggagaaaa ggcctctgct ccgcaagttt tggcgcaggc
     5581 gcaaacaggc ttatcgcacc agtacgaatt cagccgtctc acggtccatg tggacgtgaa
     5641 agcccccgct cacaggatga acccacaggc ccacgccggc gtcagggaga ggaaattcct
     5701 caggcctgga gaacagccgg cccgtagact catcgactcc gtcgatcaat ttcccaatat
     5761 gaacgtggag gtggcactgc gtgcgggtgg tatcggcgtt ataagccacg ccccatcccc
     5821 ccggccacaa ctgttccgct ttggcaatcg cggccttcca aaaatcggcc cgcgcctgag
     5881 ggctgaggtc gcccaagcgc tgtaatccct tgccgtgctg taacgggaga gcgagggtcc
     5941 ggttgggctt gcgcggattg gcatccttca aaaagaagac gccggaggat tgcttttcgg
     6001 cctcgcggca gaggctgcaa gagcgctgat cgagcgtagc cgggtcggcg tggttgcacg
     6061 cgcagtccac cgccctgacg ggaaaaggca gcaaaaagaa aaagaatagg agcggccgaa
     6121 cgggcggttt catcgcaggg cgtatttttc ccgaaccaga aagagatagc acccgatgcc
     6181 gaggaccagc cagaagccca cggccgccag cggagcatta aattttcctg tttgctcaac
     6241 cagccagcca gtaatccatg gagcggcaat gccggcgaga ctggcagcgg tgttctgtat
     6301 acccgccacc cggccaaggg ctcgagccgg taccagagtt tgtgtaaggg cccagtagtt
     6361 ggccgttgcc aggcccaggc cgcttagcga aaatacggca aaaaacagag tggccggggc
     6421 cgagtcggct acagcgccga taatttccgt aaacgccagc aggaaaccga caatgatgaa
     6481 tgtcttccgc actttcacag cgttccagcc gcgggctatc agccgatcag ccagaaagcc
     6541 gccggcgatg gcgatggtcg ccatgccgcc gaacgacatg aacgtgtacc agcccgaatt
     6601 ggtcagggag aggccgtggc ggtctttgaa gtacgtgggc atccaggtca tacagaagta
     6661 gacaaaatac atgtagcaga acgtcgcgag aaaagttccc cacataacgg gactggaggc
     6721 catcacgcgc cacgagaagg agggaaccga ggcttcggaa tgcttcgagc ttgagttccc
     6781 gccggcgtcg ttttggacca gaagcaccca tggaatcagc cacaggaggc agcccgcccc
     6841 ggtgagcagg aacatccgct gccagccgta gtccctgacc aggtaagccg ccagtggagc
     6901 gccgatcgcc ggcccatact tggtgccgga aaaataaatt cccaaagcga ggccgcgctg
     6961 cttttccgga aaatgcaaac cgatgtaacg caggctggca ggagtgacaa tggattcacc
     7021 gatccccagc aagagccgca ccgccagcaa gcccgtgaac gtggctacca gggccgtgct
     7081 cgctgaaaca aggctccaga acaggaaggc ggctgtgtaa ggccatttca ccccaaagcg
     7141 atcgacgacc cagccggccg gcacctgaag cgccgcataa aaccagaaaa atgcagagag
     7201 cacgactccc gccgattccg ccgtcagcga aagcgattgc cgcaccgccg gtaaggcggt
     7261 cgaaagattg atgcggtcga tgtaagcgat gacgacgccg accgttagca agcccactac
     7321 gggccagcgc atcgagcgcg caggaacggc agcaacggct acggctgggt cggccacgga
     7381 ttcatcattt tagccgaaac ccggtaaatg gaaacaccgg aagtcgccgg acgggaggct
     7441 gcgccatgac tgggcgatcg ccgccggcac ggccccgcag gaaatcacca ttctgctttt
     7501 aaagcatcgc tatactcaag acatggcttg tcgcacggcc ggtcatcccg cagggcgaac
     7561 ggcgcgtctc ctggctgtgg gcgcgtgtct cctggtttgg ccgccggcca gcgctcagaa
     7621 cagcagcgtt gtagagggcg ccagctccgg cgccgctgcg taccgtctca tcgatgaagt
     7681 agcgaacacc cccacgctcg aaagcaacct gcgggtgctt tgcgacgaga tcggcggccg
     7741 cctgccgggc agcgacgcca tgcgccgcgc gatctcctgg gcgattgacg cctttcacaa
     7801 agccgggctg accagcgttc atacggaaag cttttcggtt ccgaattcct gggccgaagg
     7861 ggcgacgcga gccgaagtga tatcgccggt gaggtttgtg attcatgccg ccgctaccgg
     7921 gtggtcatcg ccaataccca acggcattga ggccgaggtt ctcagcgggc agcgcggcga
     7981 ggaaggcgag gtcgaacgcc tgggcgctgc cggcaagggc aaaatcatca tcatcggctc
     8041 tgaacctctg aacaattttc aggatttggg catggagcag cggcgcgcca cgattgccat
     8101 gcgggaggcg gaaaaaatcg gcgcggcagc cgttctgttc atgtccacgc ggcctcaggg
     8161 actgctgtac cgtcaagcca acctggtgga tgggcgcctc gataagctgc cgagcgcagt
     8221 cgtggcgcgc gaggacgccc aaaggcttct gcgtctgctg gaggcgggca ggcgggtgcg
     8281 gatgcggctg gcgctgccca acaaaatcgg cgggccgttt caagcgaaaa acgtggtggc
     8341 tgaaatacgc ggggccgaaa aaccggccga agtcgtcatt gtcggcgctc atctcgattc
     8401 ctgggatctc ggaaccggct gcctggataa cggctgcaac gcggcgctcg tcatcgaagc
     8461 agcccgggcg atacaggctg cacatctgcg gccgaagcgg acgattcgct tcattctctt
     8521 cgatggcgag gaggaagggc tgcttggttc gcgcgcatat gtcgcgcagc atcgcgccga
     8581 gctggataac atcgtcgcgg tggtgatcca tgatatgggc agtggccgta tctccggcta
     8641 ctcgctcggc gggcgcggcg aaatcgagcc ggcgctcgac gagatcatgg ggccggtgaa
     8701 ttatctcgga gccaccaatc acaccaacga cgcttttttc ggcactgatc atttcgactt
     8761 cctgctgcaa ggaatcccca cgctcgtcgc caatcaggac accacggtct acgttcctaa
     8821 ctatcacgcc caatccgatg atttcgacaa agttgatctg aaggaattga aggatcaggc
     8881 cacgattgcg gccgtgacgg cctataacat cgcggaccgc ccggatcgct tgggcaagcg
     8941 cctgacgcgg gcgcagatcg ccagactcct acgcgagaca cgactcgacg agcagctcaa
     9001 gtttctgggg ttgtggcaag aatgggaaag cggccggcgc ggccggcgcg atggcggcga
     9061 ctcgtccgcg cgatgatcac tcaaagagcg gcaactgtgg gtcttcgggc gcgattgcgg
     9121 gcagatactg gcgagggctg cttgccaacc cgtgccggga gcgtattctc gccactcttt
     9181 cgcgaatcat attcgcatac tcgccgcgga tatacggact gtgcgcgaac cgagcgcggt
     9241 aacgcgcggc cagctcggga aactctgcct ctaggaatgg aaaaaatacc ttctgggccg
     9301 aaggcatcag aaacaggatt ccaccgccca aatactgcgc acccgcctcg gccgcggcgg
     9361 cagccacagc gtcgagattg gtcactgcgt cggtaagccc gggaagaatc gggttcagaa
     9421 aaacacccac ggaaatgccg gccgatgcga gaagccggac tgccgccagc cgcagatcgg
     9481 ggcgcggcgc acgcggctcg agcttgcgtg ccagcgctgc gtcggtggtt gtgaccgtca
     9541 tgttgacgtg caacacattc gaccgggcga tgctcaccag caggtcgatg tcgcgggcaa
     9601 taaggttgga tttcgtcgtg atcgaaagcg tccagccgcg ctcgccggcg aaaacctcca
     9661 aaatgcttcg cgtgaccccg tagcgccgct cagccggttg gtacggatcg gtgctcgttc
     9721 ccattgcgat ctcgcctgcc gggagccggc gcagctcccg gcgcagaatc ccggccgcgt
     9781 gggccttgac atagatcttc tgctcgaaca ggtttgcgtc ttccatgccc atgaattcat
     9841 gtgtatagcg ggcgtagcag tacctacatc caaattcgca gccgcgatag gggttgaccg
     9901 tgtacgggaa cggcatgcgc gggctggagc atttgttgag aatgctgcgg cactccagtt
     9961 cgtaatacgc cacctgcgac ttcgcttccg tgcaggggct gctcgccgcc agccgcgcaa
    10021 taccgacgag gggttccggg gcgggaaaca gcatctcttt atgttcgctt tttattcgcc
    10081 cagagtcaag ccggccaagc ccccatcaga gtacactggg gaggcgggcg ccgtcgcgtc
    10141 ccgttgccat gaagaggttt ctccggctgt tgcgagaggc ggcggtatcg gccttctata
    10201 acaagtgttt cgacaccgca aagagcgcgg catattcctc tatcctttcg tttttcccgg
    10261 ctcttatggt aacagcagcg tttctcagcg gccacgtttc cgccgtggtg gatgagatat
    10321 cgatcgcact cgggcgcgtg ctgcccccgg atgcgtacga agtggccgag caatacctga
    10381 aggttcaagg cgctcgcgcc catggcctgc ttgccggggc atggttcgtg gccatttggt
    10441 cggcgtccaa cgtaatatcc tgcttgattc agggttttca aaccgcgtac aaagtgcggg
    10501 tgaaccgctc cctcatcaag agccggttgg tagcgctggc gctgctgccg atctcgggcg
    10561 tcccgctgct gctggcgacc ttactattat ttttcggtga acaggttgcg aactggctgg
    10621 cagttcattt catcgaagcc cgatggtggc tggcgccggc cggaatggct gtgcgctgga
    10681 gcgttgcgtt cgcaacgagt gtctttgtga tcggcctgct gtatcacgtc ggttccggcc
    10741 gccggcagca atggcgcttg gtttggccgg gagccgtgtt ggcgaccgga gcctggctgg
    10801 gggcgacgct gggttttgcc tggtatgtcc gcaagatggc tcgatatggc gagctttacg
    10861 gcagcatttc gaccgctgtg gtgctgctga tctggatgta tatcgtgaac ctggctgtga
    10921 tctacggctg cgaattcaat gccgcttatg agcgggaact gagcgccgca gaagctccgg
    10981 cttcttccgg gtaaccgcta tggatcgcca gcatggcgcc atggcgcggc ccgactgaaa
    11041 gaaacgagcc ccggtgtcag aagttggttt gagaggccgc tgactggccg tctccattca
    11101 gcagagtgac cgaagctccg gtcacggcgc cgggatcccc ttgaaccgta aaaggcagcg
    11161 tcagcttgaa caaactcccg aaagaggcgg ctgccgaact ggcgaaccag tttgtgaaga
    11221 tcgagccgac atcgactgaa agcgccgtgg tctggagatt cgcccccgcg gccgggttaa
    11281 attgaactcg cactgtgttg acttcccggc tggtggcaaa agcaatgata accagttgca
    11341 gggtcgaacc agagcgcgtc gccgttacgc tctggatcac cggcgctgac cgcgcaatcg
    11401 tgagcgcgcc cacaggagca ggcgatggcg ttacgtcagt cccgcccgcg ctgaaggttg
    11461 cggtcagatt tatactcccc gccaccgtcc cggtttggag cccaatctgg gcgacattgc
    11521 cgggaaattg agcggccgtc gcgccggcag ggattgtgaa cgcgacacag cggcccgctg
    11581 gaccgttgaa attcgaacaa gtgccgccgg ttgcaaattg gatcatagga tcatccggag
    11641 cccccgcggc attcggcgaa aagctgatca caagctggcc gctgataggc gccgcatagc
    11701 tttgggccag gctgatcgtg atgttaggct gctgcgccgc ggagaaaacg ccgctcgtga
    11761 tcgttacgcc ggatacgaga cctgtgccgg tgagattgaa cgtttgcccg ttaacagtca
    11821 aactcgcgct cgctccgccc ggagccgccg gagtgaagcg gatcgtaaac gccagagaat
    11881 ccccagagag cacattggcc ggcattgccg gcagccccaa gagcgcgaag cttcctgcgc
    11941 tgacgctgat gctgttgatc gcagccgggt tcgcgcccgt attcttgata aaaaattgcg
    12001 tcgtgctgtt ggaacccacg gctaccggcg gcagaggaac cgtcccgccg gcctgcacgg
    12061 ccgtagccga gggattactc tggccgaaaa gaaaggcgaa gctgggcgcc acgccggttc
    12121 ccttcaaagt gacggtctgc gtcacgttgc cggcggcaaa tgtcgcggag ccctgtgcgg
    12181 ggcctaagcc cgccggcgca aaggtgatcg tcactgtctg attcgcgttc ggcgccagat
    12241 tgaaggtggt cgctgaaagc gaaaacgggc ccgtcgcgct ccccgtcaca ctggcctgta
    12301 cgcctccgtt gttggaaacc gccacactgg ccgtggccga ggatccgacg gccacatttc
    12361 caaagtcgat ggcggaaggc gtcacagtaa tgctcacggg aagctggcct tgcccactca
    12421 ccgccaaggt caaagtggtt gtgaccggag acgcgcttcc ggcgcttgtc agcgtaaaag
    12481 taacggttcc ggactgcggc cctacgga
//
LOCUS       DASREK010000258         5831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_949967, whole genome shotgun sequence.
ACCESSION   DASREK010000258 DASREK010000000
VERSION     DASREK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5831
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949967"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..451)
                     /locus_tag="VFA54_13980"
     tRNA            complement(377..451)
                     /locus_tag="VFA54_13980"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(416..418),aa:Glu,seq:ctc)
     gene            complement(819..2027)
                     /locus_tag="VFA54_13985"
     CDS             complement(819..2027)
                     /locus_tag="VFA54_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZS51971.1"
                     /translation="MAEFVLKYADSQGQVRQQVESAVSEVELRDRYSQKGFLVYSIRP
                     RGRAGVLSGGFRRPKKLDLAQFLIFNQQFMTLIRAGLPILKSLELLSSSLSSPKLAEY
                     IRQVREDVKSGALLSDAMRAQGAFPPIYTTSIMAGEKSGSLVEVIERYVNYQKLALAV
                     RKKVLVSLLYPSLLIVLVTGLVIFLVTYVVPNFAELYASMQAELPPMTQALVYIGLAA
                     KKHILFFAAGAVLAVFLFSLWLRTERAKAQFDRLKLRLPLFGNIWIKYQVAQFSRVLS
                     TLLVGGIPLLQALETSGDSLGNQLLRNSIRSAGKLVREGRPLSAGLQQTGIFPRLAVE
                     MIEVGESTGALPAMLNSVAEFFEEDVNTRMTAALSLIEPLIMLVMGVFVAFVLVSLYL
                     PIFSLAEKIH"
     gene            2205..3086
                     /locus_tag="VFA54_13990"
     CDS             2205..3086
                     /locus_tag="VFA54_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HZS51972.1"
                     /translation="MFHLFRYVAFSLTVCATVALGQSAPAMRTRYLTSLTNTEIEEYL
                     KRNDIIFVPVGNVEVHGGLPTDCEYVGPLAFAQRMAEEADGLVLPYLAYFFPGGTVVG
                     KGTVYVTPSEGLAYLKVIARSLLRQGFRRQIYLTGHGPSPQTVSPLIREFFDETKDPI
                     LYIETGGLLRKVKGDFNKALFGAYSIVGRLEDIPLNVTQVMPEHPRDASLSKLQELGP
                     ASGAIGAYFGDPGEHGGLAKPITAEQRAAWAKEGVAMINAAVKAADIQSIVQAMRDHD
                     KYTREVILPKYGDILPK"
     gene            complement(3161..5659)
                     /locus_tag="VFA54_13995"
     CDS             complement(3161..5659)
                     /locus_tag="VFA54_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS51973.1"
                     /translation="MTFLHLWVLSLLVPAALWALWEWTRTERRFALLLKALLLAAVVI
                     ALAQPKLAVFEKKVALGLLADTSASIPLDDLRKESELIGRVNAARGQNSLHVIPFAAQ
                     TRSLRPDENPSHLELHSDGRATNLEAAIRNAIARLSEGRVPRILLVSDGLENTGSVER
                     AIYQARLLGIPVDTYALAGSQPPSLELQSISMPAQAFTGEKFHIEAVVASPQPSPAEV
                     EITAEGKSIGRAQVQLVKGPNTIRVRAQIDAAGAMPVSGVITSPELGQVRFDRVVSLR
                     RPRLLLLTQDPAEVNRHLRQVLNAARFDISEMSGPGSSAGGFPLPANLSQYQIVLAVN
                     EDLESWPAEQKQRIEQYVRDGGGFAVVAGENNIYSDRRQENDPLDKMLPATLSPPRTP
                     DGTAVILIVDKSSSMEGRKIELARLSALGVVENLRPIDQVGVLIFDNSYQWVVPVRKA
                     DNLTQIKRLISGITADGGTQIAPALSEAYRQIMRVKAPYRHIVLLTDGISEEGDSMAL
                     AKDASDNQVTISTIGLGQDVNRLYLERLAALAKGKSYFLREFEGLQQLVLRDVMEHTG
                     SSAVEKPVNVSVLRDAEILEDVGMAKAPPLLGYLKFTAKPQAETILEVDGKDPLLVRW
                     QYGLGRAAVFTSDAKARWAANWVDWPGYDRLWTNVLRDLLPRAAETEATADFDAANNQ
                     IVVRYRFRPGAEAPNPLPDLYVLGPEGFRKAVPLRRVAALTYEGRLQIDALRGLFRIR
                     PASDVAKFPEVGYYREEPELTQYGSNPELLRQIAESTGGRFNPAPNQVFDAGNRSVTT
                     AMDLWPGLLALAIVLNLAELAGRKGYTLRRLWVR"
BASE COUNT         1195 a         1779 c         1766 g         1091 t
ORIGIN      
        1 tttcggcagg agatcgcgga taccgtggcc gatccgcgcg aggtggaatc tgagctccgg
       61 tatctggcgg ccgtgctcac gaggaaatag ctaccacgga tacgagtgcg cccgggcgat
      121 gcgcttctgc acctcaacct ctcggaacgc atcttgcggg aatgcaagtt gccggcgcca
      181 ctttacggtg ttacgcccga cctgcggata tgatcaggct ctttccacga gtcgaccagc
      241 ttccggagtt gaggctgatt tcataacggg gtaaaaaccc gtatttggat gatcgccaaa
      301 cgcttgaaaa acaacgactc cggtgtgagc gaggcgtgat ctaattgctt ctaaagattt
      361 gataataaag gagatatggt agcgctaacg ggattcgaac ccgtatttga gccttgagag
      421 ggctccgtcc taacccttag acgatagcgc cgtcttcccg gtttgcgaag ctcgccgcca
      481 gccacgagcc tgggactggc gtgacttgcc ggaaactagc tccattgtaa cccaactcgt
      541 aaaacggcga tcagggacgc ttttggaaat gtgcagcccg gattgcggac ggcggatgcg
      601 gttgccgcca tttcctacag ccgtagagcg ttccggctct gcgtcccagt ggcgtgaatc
      661 gagccgggac aggccgccgc atccacgggc agtggattgg aaaatgattg gccgcgattc
      721 tgcccgcatg gccgaggcct ccagtaaatc ggttttaaaa agcgatgggc cccggctccc
      781 gccagggcaa cccgaaatcg ggatgcgagt cttgcccgtc agtgaatctt ttccgccaaa
      841 ctgaagatcg gcagatacag cgatacgaga acgaaagcga cgaaaacgcc cattacaagc
      901 ataatcagcg gctcgatcag cgacagagcc gcggtcatcc gtgtattgac gtcttcttcg
      961 aaaaattcag ccacgctgtt tagcatggcg ggaagcgcgc ccgtcgactc acccacctca
     1021 atcatctcca cggcaagccg cgggaagatg ccggtttgct gcaatcctgc ggagagcggc
     1081 cggccttccc tcacaagctt gccggcggag cggatggagt tgcgcaacag ttgatttccc
     1141 agagagtctc cggaggtttc cagggcctgc aagaggggaa taccgcccac cagcagcgtg
     1201 ctcagcacgc gggagaactg cgctacctga tacttgatcc aaatgttgcc gaacaggggc
     1261 agccgcaatt tcagccggtc aaactgcgct ttcgcgcgtt cggtgcgcag ccatagggaa
     1321 aacagaaata ccgccaggac ggctccggct gcgaaaaaca ggatgtgttt cttggccgcc
     1381 aagccgatgt acaccaatgc ctgcgtcatc ggcggcagtt cggcttgcat gctggcgtaa
     1441 agctcggcga aattcggcac gacgtaggtg accagaaata tcaccagccc ggtcaccagc
     1501 acgatcaaca gggacggata cagcagcgaa accagcactt tcttccggac agccagcgcc
     1561 agcttctggt aattcacata gcgttcgatc acctcgacca ggctgccgct tttttcgccc
     1621 gccatgatcg aagtcgtgta aatcggaggg aacgctccct gcgcccgcat cgcgtcggag
     1681 agcaatgcgc cgcttttcac gtcttcccgc acctggcgaa tgtattccgc cagcttggga
     1741 ctgctaagag aagaagaaag gagctcgagc gatttcagaa tcggcagccc ggctcgaatc
     1801 agcgtcataa actgctgatt gaagatgagg aattgcgcga ggtcgagctt cttagggcgc
     1861 cgaaaaccgc cggaaagcac tcccgcccgg ccgcgaggcc ggatcgaata aaccagaaaa
     1921 cctttctgcg agtagcggtc gcgcagctcc acttcggaca cggcgctctc cacttgctgc
     1981 cgcacctggc cttgggagtc ggcgtattta agaacgaatt cagccattca acaacctgcc
     2041 cggatgcggt ccgccgtttt cgcccgcgcc ctccgccgcc tcgccgtcat actgaaattg
     2101 acgaacgccg gccgtccggc gtggatgtaa gttggagtct ggcgggcgta tactgtgacc
     2161 gtccggaagg gcaaatggaa attccggctg caagggaggc gtgaatgttc catcttttcc
     2221 gatatgtcgc tttctcgctc acggtttgcg cgacggtggc attgggccaa agcgctccgg
     2281 cgatgcggac ccgctatttg acaagcctga ccaacacgga gatcgaagag tacctcaagc
     2341 gaaatgacat aatttttgtg ccggtcggca acgtggaggt gcatggggga ctgccgacgg
     2401 actgtgaata cgtcggtccc ctggcgttcg cacagaggat ggcggaagag gccgacgggc
     2461 tcgtccttcc ttacctggcc tacttcttcc ctggcggaac tgtggtcgga aagggaacag
     2521 tatatgtcac gccatcggaa gggctggcct acctgaaggt aatcgcccgc tccttgctgc
     2581 gccagggctt ccgtcgccag atttatctga ctgggcatgg tccctccccc cagacggtca
     2641 gcccgctaat ccgcgaattt ttcgatgaga cgaaggatcc cattctgtac attgagaccg
     2701 gcggtctgct gcggaaagtg aagggcgact tcaacaaggc gctgtttggc gcgtattcga
     2761 tcgtgggccg gctggaggac atccctctga atgtcacgca ggtgatgccg gaacacccga
     2821 gagacgccag cctgagcaag ctccaggaac tcggtccggc aagcggagca atcggagcat
     2881 atttcggaga tccgggagag catggcggac tggcgaaacc gatcacggcc gagcagcgcg
     2941 ccgcatgggc caaagaaggc gttgcgatga tcaacgccgc cgtcaaagcg gcggacattc
     3001 aaagcatcgt ccaggccatg cgggaccatg acaaatacac gcgcgaagtg attctgccta
     3061 agtacggaga tatcttaccc aagtgagggg gcgtatttat gcagcgcgga acttcgccgg
     3121 gagcgcattt tcgcggcgtc cgccatggaa caatgcgggc ttagcgcacc cacagtcgcc
     3181 gcagggtata tcctttacgc ccggccaatt cagccaggtt cagtacaatg gccagcgcca
     3241 agaggccggg ccacaagtcc atagcggtgg tgacggagcg attcccggcg tcaaatacct
     3301 ggttcggcgc ggggttaaac cggccccctg tggattcggc aatctgccgc agcagttcgg
     3361 ggttcgaccc gtattgcgtc aactcgggct cttcgcggta gtagccgact tccggaaatt
     3421 ttgcgacgtc gctcgcgggc cggatccgga acaagccgcg tagagcatct atctgcaagc
     3481 ggccctcgta ggtcagcgcc gcaacgcggc ggagaggcac agcttttcga aagccctccg
     3541 gtcccagaac gtacaaatcg ggcagcggat tgggcgcttc cgcgcccggc cggaaacggt
     3601 accgaactac aatttggttg tttgccgcat cgaaatcggc tgtagcctcg gtttccgccg
     3661 cgcgcggcaa tagatcgcgg agcacattgg tccacagcct gtcatagccg ggccaatcca
     3721 cccaattcgc cgcccagcgc gccttcgcat ccgaggtgaa aacggcggcc cgccctaacc
     3781 cgtattgcca ccgcaccagc agcggatcct tgccgtccac ctcgagaatc gtttccgcct
     3841 gcggcttggc ggtgaatttc aagtagccca gcagcggcgg cgccttcgcc atgccgacat
     3901 cttccagaat ttcggcatcc cgcagaacgc tgacgttgac aggcttttcc acggccgagg
     3961 agccggtgtg ctccatcacg tcgcgcagca caagctgctg aagcccttcg aactcccgca
     4021 gaaaatacga tttgcccttg gcgagcgccg cgaggcgctc caggtagagc cggttcacgt
     4081 cctggccaag gccgatagta gaaatcgtaa cttggttgtc ggaagcatcc ttggccagcg
     4141 ccatgctgtc gccttcctcg gagatgccgt ccgtgagtag aactatatgg cggtagggcg
     4201 ccttgacgcg catgatctgg cggtaagctt cactcagggc gggcgcgatc tgcgtgccgc
     4261 cgtcggcggt gatcccggag atcagccgct tgatctgggt caggttgtcc gccttgcgga
     4321 ccgggaccac ccattgataa gagttatcga agatcagcac tccgacctgg tcaatcggcc
     4381 gcagattctc gaccacgccc agcgcagaaa gccgggccag ctcaattttt cgtccctcca
     4441 tcgacgagga cttgtccacg atcaggatga cggctgtgcc gtccggcgtg cggggagggg
     4501 aaagggtggc ggggagcatc ttatccagcg gatcgttttc ctgcctgcgg tcggagtaaa
     4561 tattgttttc cccggccacc acggcaaacc cgccgccgtc gcgcacatat tgctcgatgc
     4621 gctgcttctg ctcggccggc caggactcca ggtcctcgtt tacggccagc acgatctggt
     4681 attgcgacag gttcgccggg agcggaaagc cccccgcgct tgagccaggg ccggacattt
     4741 ccgaaatatc gaagcgggcc gcgttgagca cctgccggag gtgcctgttg acctctgccg
     4801 gatcctgggt aagcagcaga agccgcggac ggcggagcga gacgacgcgg tcaaaccgca
     4861 cctgccccag ctccggagag gtaatcacgc cgctcacagg catcgcaccc gccgcgtcaa
     4921 tctgcgcccg cacccggatt gtgttcggcc cctttacgag ctgcacctga gcccgcccga
     4981 tggactttcc ttccgccgtg atctccactt ccgccggcga aggctgcggg ctggccacca
     5041 cagcttcaat gtgaaatttc tcgcctgtaa aggcctgcgc cggcatcgaa atggattgca
     5101 gctccaggct gggcggctgg gagccggcca gcgcgtaagt atcgacgggg atgcccagaa
     5161 ggcgggcctg gtaaatggcg cgctccacgc tgccggtgtt ctcgagcccg tccgagacga
     5221 gcaatatacg cggcacacgg ccttccgaga gccgggctat ggcgttgcgg atggcggcct
     5281 cgaggttcgt cgcccggcca tcggaatgca gctcgagatg gctggggttc tcatcgggcc
     5341 gcaagctgcg ggtttgggct gcaaaaggaa tcacgtgcag gctgttttgg ccccgggccg
     5401 cattcacgcg tccgatcagc tcggattcct tgcgcagatc gtccaaagga atgctggcgg
     5461 aggtgtccgc caatagcccc agcgccactt ttttctcgaa cacggccagc tttggctgcg
     5521 ccaaggcgat caccaccgcc gccagcagca acgccttgag aagcagggcg aagcggcgct
     5581 ccgttctggt ccattcccac agcgcccaga gtgccgccgg aaccagcagc gacagaaccc
     5641 agagatggag gaacgtcacg atcggacctc ctggggcgcc gccgtatgcg ccgcccgcga
     5701 agaggcgcca ttttccggca cggccgcggc gccttccccg gccggagcag aagcgcgcct
     5761 ggcagatacc ggcaaaatcg ggtggctgcc gtaaaggata tattcaatca gcaggcccag
     5821 cgcccccgcg g
//
LOCUS       DASREK010000259        20052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_290666, whole genome shotgun sequence.
ACCESSION   DASREK010000259 DASREK010000000
VERSION     DASREK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 20052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20052
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290666"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VFA54_14000"
     CDS             complement(<1..1221)
                     /locus_tag="VFA54_14000"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HZS51974.1"
                     /translation="MELVTLQGRVKPDNPLLRLSSRDNVAVARANLEAGDVISFEGVD
                     VRLLNSVPMGHKVAIADIPQGSPVYKYGEDIGTARQLIRRGEWVHTHNLQFDVKERDW
                     AFCSNVSDLSDIPLAEPRTFLGYPRPDGRVGTRNYIAVAGASNCAAHAVSEIEAAFRS
                     YDFRGCPIDGVVSFPHGDGCGQSEGPDTEQLRRTLDGVMDHPNVAAAIIVGLGCEVNQ
                     ISYYLREGGNGNRRGFTLQESGGTRNVVQDGAKAVRELIDQTSRIERVEVPVSKIVLG
                     TNCGGSDGFSGVTANPALGYCSDLLVKQGGVSVLAETPEIYGAEHLLTRRARTRQIGE
                     KLLRQIEWYKTYVAWFGASFDDNPSPGNKAGGLTNIAEKSLGAAIKSGTSPLSDVLEY
                     AERIKTPGFNYMNTP"
     gene            1630..3057
                     /gene="xseA"
                     /locus_tag="VFA54_14005"
     CDS             1630..3057
                     /gene="xseA"
                     /locus_tag="VFA54_14005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZS51975.1"
                     /translation="MSQFSLNFLPERRVYSISEINAAIRETLEGAFDDVWVEGEISNC
                     RQAPSGHYYFTLKDDQSQLRCVFFRQNARFLRFKPADGLAVLARGRIGVYEARGEYQL
                     SVDHLEPRGAGALQLAFEQLKKKLAAEGLFDAARKRPLPLFPRTIGLVTSPRGAVISD
                     MLRILERRFSGLHLILYPVRVQGDGAAAEICEAIQYLGGQRARRPIPGIPPDLVIVAR
                     GGGSLEDLWPFNEESVARAIAACPVPVISAVGHETDFTIADFVADLRAPTPSAAAELA
                     IRERSEFIERIAGLEDRLGRAARYHCSTRLRRLSEIGIERVLSLLRRRMDRASQRTDD
                     LDYRAREHMRRRLLEAERRLWEADARLLAHDPRRHLASQRARLEQLRTAADRLIHMRL
                     ARLSGRLESLGAQLGHLSPLRVLERGYAIVQDESGMLLRRASETSPGRNLRVRLHKGS
                     LDAQVLRVDAGAAESSDSRPHHGGK"
     gene            complement(3042..4085)
                     /locus_tag="VFA54_14010"
     CDS             complement(3042..4085)
                     /locus_tag="VFA54_14010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS51976.1"
                     /translation="MFTKRLVACLLFAVLPGWAQAPAGGNSGETAAPGNASQPNIQVQ
                     VNVVNVPVTVTDANNRFITDLEKKDFTVWEDGKQVEVRYFSRDPKMPVVVGFLLDISN
                     SARLYFKTYQEAITDLAFVLLPGDNQNRGFLGAYSTQADFLVKPTSDGQLIADKMRSL
                     KPGGGSAMLDAIYTACTKYFSVKGQPNEPKRVLIIVGDGHDNNSKVSLDQVLEAAQRA
                     QVVIYAVSTVGYGFTNDEEQNLVRLTEETGGRIERPLQGLHKDVSGYLSTPSDEGNYA
                     YKVGTGGYAAELSGKLYQAITNLAGDIEHQYILGYTPPTPFTDRKFRSVKVAVNLKGA
                     DIKIRARKGYFPP"
     gene            complement(4214..5461)
                     /locus_tag="VFA54_14015"
     CDS             complement(4214..5461)
                     /locus_tag="VFA54_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZS51977.1"
                     /translation="MSSSRFLILGGGMVAGYAAKQLVEDGLKPGELTIVSADGSIPYE
                     RPPLSKSFLAGKDQEENILINSENFYREHGIDLRLRCEAESLDPQRNTLRLRSGENLD
                     FENLVVATGAAVRKLDVPGSGLSGILYLRSLEDAKRIRGLAQSAKRAVVIGGGFIAME
                     VSAVLAQKQIETTMVLAEDQIWKRFFTPAMSRFFESYYVARGVRFLKQQHVVSFRGDG
                     AVSSAILAGGETLACDLVVAGIGVQPVTGPVAHSGIEVSDGIVVNEYLETNRRGVYAA
                     GDVANYYDTLFHKRRRVEHWDNAVSQGRHCARILLGNRSPFVHVPYFFSDVFDLSYEF
                     WGDPAGADNIVERGDLTTNSFSVWWLAGEQVSAAFTMNRPDEEREAAPRWIESKQPVS
                     SERLSRSQSIRDAVEPRPAQSSR"
     gene            5916..6149
                     /locus_tag="VFA54_14020"
     CDS             5916..6149
                     /locus_tag="VFA54_14020"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HZS51978.1"
                     /translation="MGSHEPPGRRLEDSGKVVTGPKKLIKDGPFAEAKDVVAGYIVVQ
                     ANALAHAAELAKGCPILEVGGSVGVRPVQILNL"
     gene            6324..6506
                     /locus_tag="VFA54_14025"
     CDS             6324..6506
                     /locus_tag="VFA54_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51979.1"
                     /translation="MIVWEVLPDNGATQVKMTHVGLVPGAACYGTCEKGWNFYVGESL
                     LKLPAENKGLPNQRPR"
     gene            complement(6933..7256)
                     /locus_tag="VFA54_14030"
     CDS             complement(6933..7256)
                     /locus_tag="VFA54_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51980.1"
                     /translation="MKTKLFALLLLAGSSLFAGTHVSIAVGVGGYVPAYYVAPPPPPP
                     PVTVAYVPACPGPGFTWVAGYWYPVGGHYYWHAGYWARVPYAGARWAPPRYENRRYLS
                     GYWRR"
     gene            complement(7494..8324)
                     /locus_tag="VFA54_14035"
     CDS             complement(7494..8324)
                     /locus_tag="VFA54_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZS51981.1"
                     /translation="MRLRNAFPLIAGGAALLTALYASQRPFRVYQSLEPYDNVPLPPD
                     YQEETEWVFGRLMYPSHPHARFTWRYGDWREGGTSWAQDYPRADRHFADALRRLTRIH
                     ARSAEQPVNLDDGDDVYYWPWLCAGEVGDWKLTEAQAQKLREYLLRGGFLMLDDFWGT
                     PEWERFEETMKAVFPDRPIVEIDDRDPIFHAVYNLDDRYQVPGQWALRRGTTYRNDGA
                     VPHWRAIYDDRGRIMVAIAFNSDIGDSWEWADDPSYPERYSALGIRLGVNYVVYAMTH
                     "
     gene            complement(8383..8763)
                     /locus_tag="VFA54_14040"
     CDS             complement(8383..8763)
                     /locus_tag="VFA54_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS51982.1"
                     /translation="METITHRLFTWDSVPLEVMSDVISRKLVTGERAMVAQVFLKKDA
                     VVPEHRHESEQITYILEGAMKFQLQGKEIVVRKGQVLHIPSNVPHRAVALEDTLDLDI
                     FSPIRTDWLVKDDEYLRRGEREST"
     gene            complement(8881..9975)
                     /locus_tag="VFA54_14045"
     CDS             complement(8881..9975)
                     /locus_tag="VFA54_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS51983.1"
                     /translation="MEKVRVGLIGSGFSAELHADALARNLRVEMAACASPNRKRCEKF
                     AKKWNIPKVFTDYRAMLECKDLDLVTVGAPNYLHCRIALDAASAHKHVVIEKPLAMNL
                     DEADQMIAACKNAGVKLMYAEDLCFAPKYVRAKRIVDEGGIGRVFHIRQLEKHFGPHS
                     EWFWDMERSGGGALFDMGCHGTECARWILGKPAVKSVFASMNTYVHKNRTKGEDDSTA
                     IIEFAGGAVAVIENSWAKRGGIEDALEIHGSDGVIYCDLVRGSSMLTYSEHGYGYAVE
                     KAPDTKGWTMPLFDEWLQYGYPDEMAHFVDCVLNDRQPLETGEDGKAVLEVMFACYES
                     AAAGRRIDFPYKPAFPLRPVESWLRSKERQ"
     gene            10075..11364
                     /locus_tag="VFA54_14050"
     CDS             10075..11364
                     /locus_tag="VFA54_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS51984.1"
                     /translation="MAFTSTAINGSRAPVDALPPDVAPNPAVMAEAEGVRECLQFFTR
                     EKRWINERHLELCRIPALTFQEQERATFLAKLMAEMGYEAQIDRAGNAVVPIVYNRNA
                     PFVALTAHLDTVLAPRNAAEIELRPNGVIAGPGVADNGAGLAALLAIAKALKSGPGLT
                     HCTHNLLLAANVGEEGEGNLYGMRYLCQQGPYARRICGFLVLDGASTDHITTQALGSR
                     RFEVLITGPGGHSWSDFGIVNAIHALARAITIFCDTPLPAGSRSSINIGTIEGGSSVN
                     SIPGQARAKVDIRSESNAAIEQIVSGLEDAVRMAVEEENRRGGGSVATGKVREIGSRP
                     AGELSADSRLLASLRAVDAHLGIRSHLDRASTDANVPLSLGREALTIGAGGQGGGAHT
                     PSEWFNPEGRDLGLKRIFLSLSLLLHGAAPPLPQALQ"
     gene            11578..13179
                     /locus_tag="VFA54_14055"
     CDS             11578..13179
                     /locus_tag="VFA54_14055"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZS51985.1"
                     /translation="MASSLQKVADELRDKKLLSTTLILLTLVIGILLGTLISSGVKAA
                     RQQAVAPDATPLTIPAPTKLSSSFSQIAKRIRPAVVNINTESVIKATSGRSRRRGSDD
                     EEQDPREDLFRRFFGPFGFDDPQSDQRIHSLGSGIIVDPKGYILTNYHVIEKADRIRV
                     KLLDDSNQYTARVIGSDQETDLAVIKIDANRPLPYAKVGNSDAVEVGDWAIAIGSPFG
                     FEETVTAGIISAKGREIPGGGPRRQFQKFIQTDAAINRGNSGGPLLNINGELIGINTA
                     IISSSGGYEGLGFAMPSNVAVDVYNQIIKNGKVVRGSIGITFLNENDALLRVYGAKDG
                     GVVVNEVQPGGPSERAGIKPEDIIVAIDGKPIKNGEELVSIVASSPVGRQLKLKVLRN
                     GRTMEIPVTIGDRAEVFKERLSENRENPEEPRQGTEVMFGITVSNISPEQRHQLELEE
                     PGGVLVTNVEGGSFADDVGLVKGDVILSINRKPVANVQDVRAIQRDLKPGADVAFRIM
                     RRDPTSRQVRWRGLLLAGVLPAPGQ"
     gene            13203..13694
                     /locus_tag="VFA54_14060"
     CDS             13203..13694
                     /locus_tag="VFA54_14060"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HZS51986.1"
                     /translation="MRAVRRNLIVAIVGGLTAAWLAFGQARPVPNSPKAQKSGTKKTV
                     KKKKTTASAKTLPGKAAAPAGKASLAAKPSFRTAASRKTSVRKTARAPLPHGQQHPTQ
                     ERYKQIEEALAARGYLHTAPDGRWGPDSVEALRNFQAEHQLSPTGRLDAQSLIQLGLG
                     PSE"
     gene            13704..15194
                     /locus_tag="VFA54_14065"
     CDS             13704..15194
                     /locus_tag="VFA54_14065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZS51987.1"
                     /translation="MAAPIYCNFINGRDAASARGKTFENRNPADTNDLIGLFQDSGPE
                     DARCAVAAAAEAFRTWRNVPAPRRADVLFRAGRILSERKEQLAREMTREMGKVLEEAR
                     GDVQEAIDMAFFMAGEGRRLYGQTTPSELPDKFCMTVRMPLGVCALITPWNFPMAIPS
                     WKIFPALVCGDTVVIKPASDTPLSCWNLVHILQEAGAPPGVVNLVTGSGSGVGQPLAA
                     DPDVRLVSFTGSTAVGRSVSETCAPAFKHCNLEMGGKNVIIILEDADLDLAVDGCLWG
                     AFGTTGQRCTAASRIVVEEQVYNAFLERLAGRARALRIGNGLDPSVQMGPLINEKQLH
                     SVDEYVRIGQAEGARLVCGGHPLRHGPLASGWFYAPTVFADVDPRMRIAQEEIFGPVV
                     SVIPCRGLDHAIEIGNGVAYGLSSSIYTRDVNAAFRAMREMYTGIFYVNAPTIGAEVH
                     LPFGGTKDTGNGHRESGLAALDQFSEWKSVYVDYSNRLQRAQIDTE"
     gene            15181..15942
                     /locus_tag="VFA54_14070"
     CDS             15181..15942
                     /locus_tag="VFA54_14070"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HZS51988.1"
                     /translation="MTQNSSRTGESDVWIGTSGFVYPHWRNGVFYPPGLPQKEELEFF
                     ARSFRTVELNNPFYRLPERETFAGWRRRTPPDFRFAVKASRYITHIRKLKEAREPLRT
                     FLDRARGLGAKLGPVLFQFPSAWGVDLERLRRFLKLLPKRREFAFEFRHPSWLAQPVY
                     DLLHEYKAALCLAVRQTMSPEQEIVTADFTYIRMHSGTGCDGDFTGAELGKWAARIRR
                     FLQAGIRVYIYFNNDQHGFAVRNALDLMSLLGVGR"
     gene            15996..17852
                     /locus_tag="VFA54_14075"
     CDS             15996..17852
                     /locus_tag="VFA54_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS51989.1"
                     /translation="MGQNVRFLTARLRKAAAELPYLPRALALVWHASRAWTAAWIILL
                     AAQGLLPVATVYLSRPLVNSLVAAARSGGSWTEVRRALVFVVLTAVVLLVTEALSGIE
                     GWIRTAQSELVQDHISSLVHEKSIAADLAFYDSPDFYDHLHRARREAAYRPLALLQNL
                     GNTLQNAITLAAMLVVLLRFGPWWLPLALLASTLPACYVVLVHALREYQWRLENTAEE
                     RRTWYYDWLLTARETASEIRLFGLGDHFRSAYQASRKRLRDERLGLAKDLSVAELGAG
                     IVGLLISGVALGWMVWRTARGAGTLGDLALFYQAFNQGLRLMRSLLGNVGQLYADILF
                     LGNLFAFLDLEPRVVDAVHIAPAPAKVHSEIQFRDVVFRYPGSERPALHHFNLAIPGG
                     RITAIVGPNGAGKSTLIKLLCRFYDPEAGAIEIDGVNLKDLPVQRLRSMITVLFQEPV
                     HYSATVAENIALGDLSVLPPVQAGSIPGSVRAAGCDAGADEPIARLPQGYRNLLGKWF
                     AGGAELSVGEWQRIALARAFLRRAPIIILDEPTSAMDPWAEADWLERFRRLCAGQTAV
                     IITHRFTTAMHADLIYVLEAGQIAESGSHQELLARGGRYAQWWSTQIGVTPA"
     gene            18107..19006
                     /locus_tag="VFA54_14080"
     CDS             18107..19006
                     /locus_tag="VFA54_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51990.1"
                     /translation="MRLPVLSCAVLLMAAAAPLMAVPDDLDSLLQNLKEAVESKKDPA
                     EVKQLAAAADALSRKIIAAGGDQAAIARAKEADLYCEYALFSAASAAQPAAAVDLFSA
                     LEQQNPKSKYLDQGGYERYFLALNQSGGASKIPAIAEKAIANFPNNPDLLVVAANTAI
                     VKNQNDRALALATRLLNAAGKQQKPEGVPAAEWEKKKSLEMTTGHYIAGLASAAKGQY
                     YQADQHLRAALPSLQGTAMAGPALYYLGVANYNLGKQMMNKARVLEGAKFSEQAAAVK
                     GFPLAQEAAHNSLVMRSEAGTMR"
     gene            complement(19128..19934)
                     /locus_tag="VFA54_14085"
     CDS             complement(19128..19934)
                     /locus_tag="VFA54_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51991.1"
                     /translation="MLFIFGRATYGNAKYFLGQVSGSRTPLYFPFVYAVKEPLAVHIL
                     TLFAVAFAVRRFLDAPKRGDWVRKHFAEAAFAIIVAVYWLASIGCNLNIGVRHLLPAF
                     IPVYILAAASTVRQLTSAGSKRSKSIATACLAGLLIWQAVSVTAVYPHYIAYFNELAG
                     GPEGGYRYVVDSNLDWGQDLKRLARFVEQNRIEVINLDYFGRAEPEYYLGSKYRSVQP
                     TDAARKGWIAVSASLYQSSRAEPSRDYRSWLPLSKRVAAIGHSIFVFYNE"
BASE COUNT         3938 a         6071 c         6040 g         4003 t
ORIGIN      
        1 gggcgtgttc atgtaattaa agccgggcgt cttgatgcgc tcagcgtact cgagcacgtc
       61 gctgagcggc gaggttccgc tcttgatggc cgctccgagc gatttttccg cgatattggt
      121 gagtccaccc gccttgttgc ccggcgacgg attatcgtcg aagctcgctc cgaaccatgc
      181 tacatacgtc ttgtaccatt cgatctgccg gagcagcttt tcaccgatct gtcgagtgcg
      241 ggcgcggcgc gtaagcaggt gttcggcgcc gtagatctcg ggagtttcgg ctagcacgct
      301 gacgccgccc tgcttgacca gcagatccga gcagtagccc agagccgggt tcgccgtaac
      361 gccggagaat ccgtcggacc ccccgcaatt ggttccgaga acaattttcg agacggggac
      421 ctcgacccgc tcgatccggc ttgtctggtc gattagctcg cgcacagcct tcgcgccatc
      481 ctgcacgaca ttacgcgtgc cgccgctctc ttgcagggta aagccgcgcc ggttgccatt
      541 gccgccctcg cgcaggtagt aactgatctg gttgacctcg cagccgaggc ccacgatgat
      601 ggcagccgcg acgttggggt gatccatcac cccgtcgagc gtgcgccgca actgctcggt
      661 atcgggtcct tcgctctgcc cgcagccgtc tccatgggga aacgagacca cgccgtcgat
      721 cgggcagccg cggaaatcat aggatcgaaa cgcggcttcg atctcgctga ccgcatgggc
      781 ggcgcaattg ctggcgccgg ccacggcgat atagttgcgt gttccgaccc gcccgtccgg
      841 ccgcggatag ccgagaaacg tccggggctc ggcgagcggg atatcgctca gatcggaaac
      901 attgctgcaa aaagcccagt cgcgttcctt tacgtcgaac tgaagattgt gggtgtgcac
      961 ccactctccg cgccggatga gctgccgcgc cgttccgatg tcttcgccgt atttataaac
     1021 cggagagccc tgaggaatgt ccgctattgc aaccttgtgg cccatcggca cgctgttcag
     1081 cagccggacg tcgacgcctt caaaagaaat gacgtcgccc gcctccagat tggctcgcgc
     1141 cacagcgacg ttatcgcggc tgctcagccg caacagcggg ttgtccggct tcacgcggcc
     1201 ttgcaaggtt acaagttcca tagatttttt acgcaattta ttttcccaga aagggttccg
     1261 cgcagcaacc gccgataacc tttcgcggga tccgtcgtct atgtaatcag aggacgaaat
     1321 gaaactgaag ctgcgatact ggatcctctg tggagcgctc ggtatgaccc tgctttcagc
     1381 cgcgcggtcc gtttcggctt cttcggccta tcaggcttac ctagccggcc gctcttcggg
     1441 agcggtgctt ttctccatcg tacaggcttt tgggatgcag gcgtctcgct gtgagcctcc
     1501 gcaccatatt ccggcccgcc tctcctgtac tcgctgccgc cgctgatcga gtccctgccg
     1561 cttcccaaca cgccgctcgg attgcgggtc tccgggacaa tcggctattc ttcaattgat
     1621 cggacgcgca tgtctcaatt ttccctgaac tttcttcctg agcgcagagt ctattcgatc
     1681 agcgaaatca acgcggcaat tcgcgaaaca ctcgagggag cctttgacga cgtctgggtg
     1741 gaaggtgaaa tatccaattg ccgccaggcc ccgagcggcc actactactt cacgctgaaa
     1801 gatgatcagt cgcagcttcg ctgtgttttt ttccggcaga acgcgcgctt tctgcggttc
     1861 aagcccgccg acggtctggc ggtgctcgcg cgggggcgga tcggcgtgta tgaagcgcgc
     1921 ggcgaatacc agctttccgt cgaccacctc gagccgcgcg gcgccggcgc cttgcagctt
     1981 gccttcgagc aactgaaaaa aaagctggct gcggaagggc tttttgacgc agcgcgcaag
     2041 cgtcccctgc ctctttttcc gcgcactatt ggcttggtca cttcaccgcg gggcgcagtc
     2101 atctccgata tgctgcgcat actggagcgc cgcttttccg gtttgcattt gatcctttat
     2161 cccgtccgcg tgcagggaga cggagcggcg gcggaaattt gcgaagccat ccaatattta
     2221 ggcggtcaaa gggcgcggcg gcccatccct ggcattccgc ccgacttggt catcgtcgcg
     2281 cgcggcggcg gctcgctcga agacctttgg cctttcaatg aagagagcgt ggcgcgcgcc
     2341 atcgctgcct gccccgtgcc agtgatttcc gccgttggcc acgaaaccga tttcaccatc
     2401 gccgattttg tcgccgatct ccgggcgccg accccttcgg ccgccgcgga actggcgatc
     2461 cgggaacgat cagaatttat tgagagaatc gccggtctcg aagatcgtct cggccgcgct
     2521 gcccgctatc attgcagcac ccggcttcgc cggctcagtg aaatcggcat cgagcgggtc
     2581 ctcagcctgc tgcgccggcg catggaccgc gccagccagc gcaccgacga tctcgattat
     2641 cgcgctcgcg agcatatgcg ccgccggctg ctcgaggccg aacgccggct gtgggaggcc
     2701 gatgcgcggc tgctggctca cgacccacgc cgccatctgg ccagccagcg ggctcgcctg
     2761 gagcagttgc ggacggcggc cgaccgcctg atacatatgc gcctggcccg cctttcgggc
     2821 aggctcgaat ccctcggcgc gcaactgggt catctcagcc ctctgcgcgt gctcgagcgc
     2881 ggctacgcaa ttgtgcagga cgaaagcgga atgctcctcc ggcgcgcgtc ggaaacctct
     2941 cccggacgca acttgcgcgt acggctgcac aaaggaagtc tggatgcgca ggtgctgcgg
     3001 gtcgacgccg gggcggcgga aagcagcgat tcccggccgc atcacggcgg gaaatagccc
     3061 ttgcgggcgc ggatttttat gtcggctcct ttgaggttga cggccacctt cacgctgcgg
     3121 aacttccggt cagtaaaggg tgtgggcggc gtgtagccca atatgtattg gtgctcaata
     3181 tcgcccgcga gattggtgat cgcctggtaa agcttgccgg agagctcggc ggcataaccg
     3241 ccggtgccga ctttataggc atagttgcct tcgtcggaag gagtggaaag gtagcctgag
     3301 acgtccttgt gaagcccctg caaaggccgc tcgatgcgcc cgccagtttc ttcggtgagc
     3361 cgcaccaggt tctgttcctc gtcgttcgtg aacccgtagc cgactgtgct tacagcatag
     3421 atgacgacct gcgcccgctg ggccgcctcc aggacctgat cgagcgacac cttgctgttg
     3481 ttgtcgtggc cgtcaccgac gatgatcagc acgcgcttgg gctcgttagg ctgtcctttt
     3541 acggagaaat acttcgtgca agccgtatag atcgcgtcca gcatcgcgct gccgccgccc
     3601 ggcttcaggc tgcgcatttt gtccgcgata agctgcccgt cggacgtggg cttcaccaga
     3661 aaatcggctt gcgtggagta ggcgcctaag aatcccctgt tttggttgtc acccggcaga
     3721 agcacaaagg ccaaatcggt gatcgcttcc tgataggtct tgaagtaaag gcgagccgag
     3781 ttgctgatgt ccagcagaaa accgaccacc accggcattt tcggatcgcg ggagaaatag
     3841 cgaacctcca cttgcttgcc gtcttcccac acagtgaagt ccttcttctc cagatcggta
     3901 atgaaacggt tgtttgcatc ggtaaccgtc accggcacgt tgacgacgtt gacctggacc
     3961 tggatgttcg gctgtgaagc attcccagga gcggccgttt ctccggagtt cccgccagcc
     4021 ggcgcttgag cccagccagg aagaactgcg aatagcagac aggccacaag gcgcttggtg
     4081 aacatagccc accttctcgg gactattgta gccgatctgc caacctgcgg aatacctact
     4141 gcggagctcc gtccacggcc cggagacgaa agtccacacg cccttatcag agggaacact
     4201 cacacatggc gcttcatctg ctgctttgag ccggccgggg ctccacggcg tcccgtatgg
     4261 attgcgatcg gctcagtcgt tcggaggata caggctgttt cgattcaatc cagcgcggag
     4321 ccgcctcgcg ctcttcgtcc gggcggttca ttgtgaacgc cgcgctgact tgctctccgg
     4381 ccagccacca gacgctgaaa ctgttggtcg tcaaatcccc acgctcgaca atgttatccg
     4441 cgcctgcggg gtctccccag aactcgtagg aaagatcaaa gacgtccgag aaaaaatagg
     4501 gcacatgcac gaaaggggag cgattcccca gcagaatccg ggcgcagtgc cgcccttgcg
     4561 ataccgcatt atcccagtgc tccacgcggc gccgcttgtg gaacagcgtg tcgtaatagt
     4621 tcgccacatc gcctgccgca taaacgccgc ggcgattcgt ctccagatac tcgttcacga
     4681 caatgccgtc cgacacttcg atgccgctat gcgcgactgg gccggtcaca ggctgcacgc
     4741 cgatgcctgc gaccaccaaa tcgcaggcaa gggtctcgcc gcccgccaga attgccgagc
     4801 ttacggcgcc atcgccccga aacgagacaa cgtgctgttg cttcaggaag cgcacgccgc
     4861 gcgcaacgta gtagctctca aaaaagcgcg acatggcggg cgtaaaaaac cgtttccaga
     4921 tctgatcctc ggccaaaacc atggtggtct cgatctgctt ttgcgccaat accgcagaga
     4981 cctccatggc gataaagccc ccgccgataa ccaccgcccg tttcgcgctc tgcgccaggc
     5041 cgcgaatccg tttggcatcc tccagtgagc ggagatacag gatccccgag aggccgctgc
     5101 ccggaacatc cagcttccgg accgctgctc ccgtcgctac tacgagattt tcgaaatcca
     5161 ggttttcacc ggagcgcagc cgcagggtgt ttcgttgcgg atcgaggctt tccgcctcgc
     5221 aacggagccg caaatcaatc ccgtgctccc ggtagaagtt ttcggaattg atgaggatat
     5281 tttcttcctg gtcttttccc gccagaaagc ttttggacag gggaggccgc tcgtagggaa
     5341 tggaaccgtc agcggatact atcgttagct cccctggttt caatccgtcc tcgacgagct
     5401 gtttggctgc gtacccggcc accatcccgc cgcccagaat gaggaatctc gaagatgaca
     5461 tggcgcgctc ctgatgagaa ttttatccag tctgattcat tgcatcataa ccggacggaa
     5521 gatctcgcgg aagaagacac gaactcgcct ttctcttcgc gggatcaggc cggatcgggc
     5581 gcacttggac aggccgaatt cgccgatgga gaaaacgggt tgggaaggcc ccgggcgacg
     5641 agcagcgagc agaagcaacg aaatcgctga actgatgccc gcagcatccg gaagtgcggc
     5701 ctctgcgaaa ttccggaaaa gattttgtcc ggccatcaga ccctcgcacg tcatacgctt
     5761 gaaacagcgc gacgctgctc gttagtgatg cgcctggcgg ctgttcccaa atcgagaaca
     5821 aggagaatca tgagcgaatt tacgtatctg tttcgtggca ggaatatacc cgcctctccc
     5881 gaagagcggc agaaacattt ggataaatgg gccggatggg atcacatgaa ccacccgggc
     5941 gccgcctgga ggacagcggc aaggttgtga ccggccctaa gaagttgatc aaggatgggc
     6001 cgttcgccga ggccaaggat gtcgtggccg gctatattgt cgtgcaggcg aacgctctcg
     6061 cgcatgcggc ggaactggcc aagggctgtc cgattctgga ggtcggcggc tcggttggag
     6121 ttcggccggt gcagattctg aacctgtgag ttggtgtcgc cagtcagagg aaagctgttt
     6181 tatctcacta aagcgcgatt gaagatattc aataaggagt gatcatggca agggcaattt
     6241 ttggaaatta tacggcggtt tttgccgcgc gagccgattg caacctccac tagatcgacg
     6301 acaagaagga gtggaaagac ggcttgatcg tgtgggaagt cttgcccgac aacggagcga
     6361 cgcaggtgaa gatgacgcat gtcggcttgg ttccgggcgc cgcgtgttac ggcacctgcg
     6421 agaaaggctg gaacttctat gtcggggaaa gcctgctgaa actgccggcc gaaaacaaag
     6481 gccttcccaa ccagcggcca cgatgacaga gcaggtggcc atcaccggtt cacaggttat
     6541 ggagcggttg agcagaggca ttgattgagt atatgtgcgg atcagcgcac ccgcaggccg
     6601 atcagggcgc gcccgcccga ggagcggtgt cgtcgtcggc ggccacccac ggcgcggcac
     6661 aagttctgtt cacaaccgtt ttccttaacg gggctgaaat attcgatcgc actcgcacaa
     6721 ctgcctgggg gtcctgccgg ataacgcgcc gctcagtcga gcgccgtatc gagattgggc
     6781 acgtcgatga tgaagaaccc gccgagctgt tccttcctaa tcagcatgag gacctcctgc
     6841 atggggcttt ccgattcgct cggacggggc tccggccagc gggagccccg ttcccgcttt
     6901 ttcaggatgc acagcttggc cgccgttcct agctaccgtc tccaatatcc ggagagatag
     6961 cggcggtttt cgtatcgcgg cggcgcccaa cgcgcaccgg cataaggcac tcgagcccag
     7021 tagccagcgt gccagtaata gtgtccgccc accgggtacc aatatccggc aacccacgtg
     7081 aaacccgggc cggggcaggc cggaacataa gcgactgtga ccggcggcgg aggcggtggc
     7141 ggcgctacgt aatatgccgg aacatagcct ccgacgccaa ccgcgatcga aacgtgcgtt
     7201 ccagcgaata aggagcttcc ggccaagagc aggagtgcaa acaatttagt tttcatatgt
     7261 cgtctcctcc ttccactgtt gccgctttgc cgcaacggct ttcaattctg tttcatagca
     7321 aacggagtgc cagggataaa tcgctgaaaa cacggcccga ctcgatcagc ctctggtttt
     7381 gatccaccgc tctggtgctt ttcagccaat atcccgcgca gcgtaggcga aaggccctcc
     7441 tctgggcggc gcgaccggca agaaggacgt ctcagagcgg tttccggcgg tgatcagtgg
     7501 gtcatggcgt aaaccacgta gttcacgccc aggcggattc ccagtgcgga gtatctttcg
     7561 gggtaggaag ggtcgtcggc ccactcccag gagtcgccga tatccgaatt gaaggcaatg
     7621 gccaccatga tccgcccgcg gtcgtcgtaa atagcgcgcc agtggggaac ggcgccgtcg
     7681 tttcggtaag tggtcccgcg acgcagcgcc cattggccgg ggacctggta gcgatcgtcc
     7741 aggttatata cggcgtggaa aatcgggtcg cggtcgtcga tttcgacgat cgggcggtcg
     7801 gggaacacgg ccttcatggt ttcctcaaag cgctcccact cgggtgtgcc ccaaaaatcg
     7861 tccaacataa gaaaaccgcc gcgcagcaga tactcccgaa gtttctgggc ctgcgcttcc
     7921 gtcaatttcc agtcgccgac ttcaccggcg cacaaccagg gccagtagta tacgtcgtct
     7981 ccgtcgtcga gattcacggg ctgttcggcc gagcgcgcat ggatgcgggt gagacgccga
     8041 agcgcatccg caaagtgtcg gtccgcccga ggataatcct gggcccaact cgtccctccc
     8101 tcgcgccagt ccccgtagcg ccacgtgaaa cgggcgtgcg ggtgagaggg atacatgaga
     8161 cggccgaaaa cccactccgt ttcttcctga taatccggcg ggagcgggac attatcgtag
     8221 ggctccaggc tttggtaaac acgaaagggc cgctgggatg cgtacaaggc ggtgagcagg
     8281 gcggctcctc cggctatgag cgggaaggcg ttgcgcaggc gcatgagctt tccttgagaa
     8341 tatcaccgcc gcaccgcttc ctcccaggcc gccgcggccg cttcatgtcg attcgcgttc
     8401 tccccgccgc aggtattcgt catctttgac gagccagtcc gtgcgaatcg gtgaaaagat
     8461 gtccagatcc agcgtgtctt cgagcgcaac cgcgcgatga gggacattgg aagggatgtg
     8521 aaggacctgt cctttgcgca cgacgatctc cttgccctgg agctggaact tcatggctcc
     8581 ttcgagaatg taggtgatct gttcgctttc gtgacgatgc tcgggtacga cggcgtcttt
     8641 cttcaaaaag acctgcgcaa ccatggcccg ttcgccggtc accaatttgc gcgaaatgac
     8701 atcgctcatg acctcgagcg gcacggagtc ccacgtaaaa agccggtgcg tgatggtttc
     8761 catttcgacc tattgtagaa ggttttgcgc ttcgcagccg gctgggagat tccggctttc
     8821 cgggtcccga atgcgagttc gccacccgaa acccgaacct tttgaccgac tctccgccgc
     8881 ctactgacgc tccttcgagc gcagccagga ttcgacagga cgcaaaggga aggccggctt
     8941 gtaagggaaa tcgatgcggc ggccggccgc cgccgattcg taacaggcga acatcacctc
     9001 caggacggcc ttgccgtctt cgccggtttc gagcggctgc ctgtcgttga ggacgcaatc
     9061 aacaaagtgc gccatttcgt cgggatatcc atattggagc cactcatcga acagcggcat
     9121 cgtccaccct ttcgtgtccg gagctttttc aaccgcatag ccgtacccgt gttcgctgta
     9181 ggtcagcatg gaactgcccc gcaccagatc gcaataaatc acgccgtcag agccgtgtat
     9241 ttccagggcg tcttcgatgc cgccgcgctt cgcccagcta ttctcgataa cggctacggc
     9301 gccgccggca aactcgatga tggccgttga gtcatcctct cctttggtcc ggttcttgtg
     9361 aacgtaagta ttcatggagg cgaacacgga cttcacagcg ggctttccca agatccagcg
     9421 ggcgcattcc gtgccgtggc aacccatatc gaacagagcg ccgccgccag atcgctccat
     9481 atcccaaaac cattccgaat ggggcccgaa gtgcttctct aactgccgga tatgaaagac
     9541 cctaccgatg ccgccctcgt ctacaatgcg cttggcgcgg acgtatttag gcgcgaaaca
     9601 caggtcctcc gcgtacatca gtttcacgcc cgcgtttttg caggcggcga tcatctggtc
     9661 cgcctcgtcg aggttcatgg ccaatggttt ctcgattacc acgtgcttgt gcgccgacgc
     9721 ggcgtcgagc gcgatccggc aatgcaggta gttgggcgcg cccacggtaa ccagatccag
     9781 gtctttgcac tcgagcatgg cccggtagtc agtaaaaact ttgggaatgt tccatttttt
     9841 ggcgaatttc tcgcacctct tgcgattcgg cgacgcgcag gccgccattt ccacgcgcaa
     9901 gttgcgcgca agagcgtccg cgtgcaattc cgcggaaaac ccagagccga tcaggcctac
     9961 tcgaaccttc tccatttgca tctccctccg gggtagtgta gcagcaccga atgtcatgca
    10021 tcgccgcatt gggtctcgcc gcgccgcgtt gagacggcat gctagaatcg atgcgtggca
    10081 ttcacttcca cagcgatcaa cggatcgcgc gcaccggtag atgcgctgcc cccggacgtg
    10141 gcgccgaatc ctgccgtcat ggccgaggcg gagggcgtgc gggagtgcct ccagtttttc
    10201 acccgcgaaa agagatggat caacgagcgc cacttggagt tgtgcaggat tccggcgctc
    10261 acctttcagg agcaggaacg ggccactttc ctcgcaaagc tgatggccga aatgggttac
    10321 gaggcgcaga ttgaccgcgc cggaaacgcg gttgtgccga tcgtatacaa ccgcaacgcg
    10381 ccttttgtag cccttacggc tcatctcgat acggtactgg cgccgcggaa tgccgccgag
    10441 atcgagctac ggcccaacgg cgttatcgcc ggtccgggcg ttgccgacaa tggagccggc
    10501 ctggcggctc tgctcgccat cgccaaagcg ctcaaaagcg gccccggcct gacccattgc
    10561 acgcacaatc tgcttctggc ggcgaatgtg ggggaggagg gcgaaggaaa cctctatggg
    10621 atgcggtatt tatgccagca agggccctat gcccgccgca tctgcggatt tctcgtgctg
    10681 gatggagcct cgacagacca tatcaccacg caggcactgg gcagccgccg gttcgaggtg
    10741 ctgattacag gtcccggcgg ccatagctgg agcgatttcg gcatcgtcaa cgcgattcac
    10801 gctctggcga gggccatcac gatcttttgc gacaccccgc ttccagccgg atcgcgctcc
    10861 tcgatcaaca taggcaccat cgagggcggc agcagcgtga attcgattcc cggccaggct
    10921 cgcgcaaagg tggatattcg ctccgaatcc aacgccgcga tagagcagat tgtctcggga
    10981 ctcgaggatg ctgtccgtat ggcggtcgaa gaagagaacc ggcgcggcgg gggcagcgtg
    11041 gccacgggta aggtgcggga gatcggctcg cgtccggcgg gagaactttc tgccgattca
    11101 cgattgctgg cttccctgcg ggcggtggat gcccacttgg gtatccgctc ccatctggat
    11161 cgggcctcta ccgacgccaa cgtgcctctc tcgctcggcc gcgaggccct cactatcggg
    11221 gcaggcggtc aggggggcgg cgcccatacc ccttccgagt ggtttaaccc cgaaggccgc
    11281 gatttgggct tgaagcggat ctttctttcg ctttcgctat tgctgcacgg ggctgccccg
    11341 cctttacccc aggcgctcca atagcaaccc cggcaggccc tgcgctggct taaattgcgc
    11401 tctgctgaaa cctggctgtc atggcaaaca tcctttcctt tgtctgttga gatcggggca
    11461 tgcggccata tagcaggtca gtccggcaaa cgtttacccc cccaggggct ccgtgtgcca
    11521 gtccgggtcc tgtaatacaa tgaagaaagt cttagattgc gggaaggaag gtgtccgatg
    11581 gcatcgagct tacagaaagt tgcggacgaa ttgagggaca agaagctcct ttcaactact
    11641 ttgattctgc tcacgctcgt gattggcatt ttgctgggga ctctcatctc gagtggggtc
    11701 aaggcggcgc ggcagcaggc cgtcgcgccg gatgcaacgc ctctgacgat tcccgctcct
    11761 accaagctga gcagcagctt ctcgcaaatc gcgaaaagaa ttcgtccggc ggtcgtcaat
    11821 atcaatacgg agtccgtcat caaggcgacc agcggccgaa gccgccggcg cggttcggat
    11881 gacgaggaac aggatcctcg ggaagacctg tttcggcgat tcttcgggcc ttttggtttc
    11941 gacgatccgc agagtgatca gcgcatacac agcctggggt cgggcatcat cgtggatccc
    12001 aagggttata tcctgacgaa ctaccatgtg atcgagaagg ccgaccggat tcgtgttaaa
    12061 ttgctcgatg actccaatca atacaccgcg cgggtgatcg gttcggacca ggaaaccgac
    12121 ctggccgtta ttaagattga cgccaaccgt ccgctgccct acgcgaaggt cggcaattcc
    12181 gatgccgtcg aagttggcga ctgggccatt gccattggat ctccgttcgg ctttgaagag
    12241 acggttacgg ccggcattat aagcgctaag gggcgggaga tccccggcgg aggtccgcgg
    12301 cgccagttcc aaaaattcat tcaaaccgat gccgcgatca atcggggcaa cagcggcggc
    12361 ccgctgctga acatcaacgg cgaactgatt ggcatcaata cggccatcat ttcttcgagc
    12421 ggcggctacg agggcctggg tttcgccatg ccgtccaatg ttgccgtgga cgtctacaac
    12481 cagatcatca agaacggcaa ggtagtgagg gggtcgatcg ggatcacgtt cctgaacgaa
    12541 aacgacgccc tcctgcgcgt ttatggcgcg aaagacggcg gagttgtggt caatgaggtc
    12601 cagccgggcg ggccgtcaga gcgagcgggc atcaaaccgg aagacatcat cgtcgccatt
    12661 gacggcaagc ccataaagaa cggcgaagaa ctggtttcga tcgtggcctc cagcccggtg
    12721 ggccggcagt tgaagctgaa ggtgttgcgc aacggcagga cgatggaaat tcccgttacg
    12781 atcggcgacc gggccgaagt tttcaaagag cggctcagcg agaaccgcga gaacccagag
    12841 gagccccggc aggggaccga agtgatgttc ggcattacgg tatccaacat cagccccgag
    12901 cagcgacatc aacttgagct cgaggagccg ggcggtgtgt tggtaaccaa cgtggaaggc
    12961 ggctccttcg ccgacgatgt gggtcttgtg aagggagatg tgattctgtc catcaaccgg
    13021 aagccagtgg ccaatgtgca agacgtacgg gcgatccagc gcgacctcaa accgggggct
    13081 gatgttgcgt tccgcatcat gcggcgtgat ccaacgagcc ggcaggttcg ctggcgtggc
    13141 ttgttgctgg ccggcgttct ccctgcaccc ggccaataaa aagttgaacg gcagacgttt
    13201 ccgtgagagc ggtccggcga aatctgatcg ttgcgattgt cggcgggctg actgcggcat
    13261 ggttggcttt cggccaggct cggcctgttc cgaacagccc gaaggcccag aagtcgggga
    13321 ctaagaaaac agtcaaaaag aagaaaacaa ccgcctccgc caagactctc ccgggcaagg
    13381 cggcggctcc tgcgggaaag gcctctttgg ccgccaagcc ctcttttcgc acagccgcca
    13441 gccgcaaaac cagcgtccgg aaaacagctc gggccccgct tccacacggg cagcaacacc
    13501 cgacccagga acgttataag cagatcgagg aagctttggc ggcgcgcggc tatctgcata
    13561 cggcgccgga cggccggtgg ggccccgact cggtagaggc tctgcgcaat tttcaagccg
    13621 agcaccaact gtcccctaca ggccggctgg atgcgcaatc gctcattcaa ctgggactag
    13681 gaccttccga ataggagctg cctatggcgg cgcccatcta ttgcaatttc atcaacggcc
    13741 gggatgcggc atcggcccgc ggaaagacct tcgaaaaccg caatccagcc gacaccaatg
    13801 acctgatcgg ccttttccag gactccgggc cggaagacgc gcgctgcgct gtcgccgccg
    13861 cggcggaagc ctttcggacc tggcgcaacg tgcccgcgcc ccggcgggcc gacgtcctgt
    13921 tccgggcggg ccgcattctc tcggaacgga aagaacaact ggcgcgggag atgacgcgcg
    13981 aaatgggaaa ggtgctcgaa gaggcccggg gcgatgtcca agaagcgatt gatatggctt
    14041 ttttcatggc aggggaaggc cgccgccttt acggtcagac cacgccctcg gagcttccgg
    14101 ataaattttg catgaccgta cgcatgcccc tcggcgtctg cgcgctgatt actccatgga
    14161 acttcccgat ggcgatccct tcctggaaga ttttccccgc cctcgtttgc ggcgatacgg
    14221 tggtgatcaa gccggcatcg gacacgcctc tgagttgttg gaacctcgtc cacattctcc
    14281 aggaggcggg cgcgccgccc ggcgtggtca atctggtcac cgggagcggc agcggcgtgg
    14341 gccagccgct ggcggcggat ccggatgtgc ggctcgtctc gtttacggga tcgacggccg
    14401 taggtcgaag cgtttctgag acctgcgccc ctgccttcaa gcactgcaat ctggaaatgg
    14461 gcggcaagaa cgtgatcatc atcctcgagg acgccgatct cgacttggca gtggatggat
    14521 gcctgtgggg cgccttcggc acaaccggcc agcgatgcac agccgccagc cgcatcgtcg
    14581 tcgaagagca ggtttataac gcgttcctag aacgccttgc tggccgcgcg cgtgctctgc
    14641 ggatcgggaa tggcctcgat ccttcagtcc agatgggacc cctcatcaat gaaaagcagc
    14701 ttcatagtgt ggacgaatat gttcgaatcg ggcaggccga aggagcccgg cttgtttgcg
    14761 gcggacatcc gctgcgccac ggccctttgg cttccggctg gttttacgct cccaccgtct
    14821 ttgctgatgt ggatccgagg atgcgcatcg cccaggagga aattttcggg cccgtggtct
    14881 ctgtgatccc ttgccgcggc ctcgatcatg cgatcgaaat cggcaacggc gtcgcgtatg
    14941 gcctttcttc ttcgatttat acccgcgatg tcaacgccgc gtttcgggct atgcgggaga
    15001 tgtataccgg catcttttat gtcaatgcgc caacgatcgg ggccgaggtg cacctgccgt
    15061 ttgggggcac caaagacacc ggcaatggtc accgggaaag cggactggcc gctctcgacc
    15121 agttctcgga gtggaaatcc gtttacgtag attacagcaa cagactccag cgcgcccaga
    15181 ttgacacaga atagctccag gaccggcgag agcgatgtct ggatcgggac ttcgggcttt
    15241 gtctatccgc attggcgaaa cggggtgttt tatccgccgg gactgcctca gaaagaggag
    15301 cttgaatttt ttgcgcggtc cttccgcacg gtcgaactga ataacccctt ttatcgcctt
    15361 cccgaaagag agacattcgc cggctggcgc cgccgcacgc cgccggattt tcgttttgcg
    15421 gtaaaagcca gccggtacat cacccatatc agaaagctga aagaagcccg ggaaccgctg
    15481 cggacctttc tcgaccgcgc gcgcggactt ggcgcaaagc tggggcccgt gctgttccag
    15541 tttccgtctg cctggggagt ggatctggaa cggctgcggc ggttcttgaa actgctgccc
    15601 aagcgcaggg agttcgcctt cgagttccgg cacccgagct ggctggctca gcccgtttat
    15661 gacctgctgc acgagtacaa agcggcgtta tgtcttgccg tgagacaaac catgtcgccg
    15721 gaacaggaaa ttgtgaccgc ggatttcacc tatattcgca tgcacagcgg aacgggatgc
    15781 gatggcgact ttaccggcgc ggaactgggg aaatgggcgg cacgcatccg gcgctttctc
    15841 caggccggca tccgcgtcta catctacttc aataacgacc agcacggctt tgccgttcgc
    15901 aatgccctcg atctcatgag tttgctgggc gtcgggcgct gagggcccgg atttcatgcc
    15961 ggcaaaaggg ccggctaatg agatgatatt gcctggtggg ccaaaatgtc cgatttctga
    16021 ctgcccggct gcgtaaagcg gcggccgaac tgccgtattt gccgcgagcg ctggcgctcg
    16081 tgtggcacgc atcgcgcgcc tggacagccg cctggatcat cttgctggcc gcgcaaggac
    16141 tgctgcctgt cgccaccgtg tatctcagcc ggcctttggt gaacagcctg gtggcggccg
    16201 cccgcagcgg cggctcgtgg accgaagtgc ggcgcgcgct ggtcttcgtg gtgctgaccg
    16261 cggtggttct attggtgacc gaggctcttt ccggcataga aggctggatc cgaacggccc
    16321 aatcggagct ggtgcaggac cacatcagca gcctggtgca cgaaaagtcc attgcggccg
    16381 atctggcttt ctacgattcg cccgattttt acgatcacct ccaccgcgcg cgccgcgaag
    16441 cagcataccg gccgcttgcc ttactgcaaa atctgggaaa cacgttgcag aatgccatta
    16501 ccctggcggc catgctggtg gttttgctgc gcttcggacc ctggtggctg ccgctggctc
    16561 tactcgccag cacgctgccg gcctgctacg tcgttttagt acacgcgttg cgggaatatc
    16621 agtggcggct ggaaaacacc gccgaagagc gccgcacttg gtattacgat tggctgttga
    16681 cggcccggga gacggcgagc gaaatccggc ttttcggtct tggcgaccac ttccgttcgg
    16741 cctatcaggc gtcccgcaag cggcttcgcg acgagcgtct cggtcttgcc aaggatctga
    16801 gtgtggcgga actcggcgcg gggatcgtcg gccttctaat cagcggcgtc gctctggggt
    16861 ggatggtgtg gcgtaccgca cgaggagcag ggaccctggg cgacctggcg ttgttctatc
    16921 aagccttcaa ccagggctta cggctcatgc gatcgctgct ggggaatgtc ggtcaactct
    16981 acgcggatat tttgtttctt ggaaacctgt tcgcgttttt ggatctcgag ccgcgcgtgg
    17041 tggacgctgt gcatatcgcg cccgcccccg ccaaggtgca ctcggaaatt cagtttcggg
    17101 atgtcgtctt ccgctatccc ggcagcgagc gtcccgccct gcatcatttc aatctcgcga
    17161 ttcccggggg aaggatcacc gccatcgtcg gtcccaatgg agcggggaaa agtacgctga
    17221 tcaagctgct ctgccggttt tacgatcccg aggccggagc gatcgagatt gatggggtga
    17281 atctcaagga ccttcccgtg cagcggcttc ggtccatgat cacagtcctg ttccaggaac
    17341 cggtgcatta cagcgcaacc gtggcggaaa acatcgccct cggcgacctc tccgttctgc
    17401 cgccggtgca agcgggaagt ataccgggct ccgttcgagc ggccggctgc gatgctggag
    17461 ccgacgagcc tatcgcccgc ctgccgcagg gctatcgcaa tctgttggga aaatggttcg
    17521 ccggcggcgc ggaactgagc gttggcgagt ggcaaagaat cgcgctcgcc cgggcgtttt
    17581 tgcgccgcgc tccaatcatc attctcgacg agccgacgag cgcgatggat ccctgggccg
    17641 aagccgactg gcttgagcgc ttccgccgct tgtgcgccgg ccagacggct gtcatcatca
    17701 cccatcgctt cacaactgca atgcatgcag acttaattta cgtcctcgaa gcaggtcaaa
    17761 tcgccgaatc cggatcccac caggagttgc tggctcgcgg cggccgctat gcccaatggt
    17821 ggtcgacgca gattggcgtg acgccggcct aaaccgcagg cgccaaaccc ggcggcatcc
    17881 tagaagccct ggcgccgccg cgtgtccgcc ggatttcgcg cgggtcgacg gtggcggtcc
    17941 tacgatgcgg ctgtcacttg ggaactcgtc cacctcggcg gtcggctctc gattcagagg
    18001 gccgggcagt ctgccggagc ccggcaatat ttgctcctgc tgctttcccg gccgattcgt
    18061 tcggcctata atacggccaa acccccgggg cagctaggag gcctgtatga gactccccgt
    18121 attgagctgc gctgttctgc tcatggcggc ggccgcaccc ctcatggcgg ttcccgatga
    18181 tctggacagc ttgctgcaaa atctcaaaga agccgtcgaa tcgaaaaaag atcccgctga
    18241 ggtgaaacaa ctggcggcgg cagccgatgc tttgtctcgc aagataattg cggccggagg
    18301 cgatcaggcc gccatcgccc gtgccaaaga ggcggatctg tactgcgagt atgctctctt
    18361 ctccgctgct tcggccgctc agcccgcggc ggccgtggac ctgttttccg ccctcgagca
    18421 gcagaatccc aagagcaagt acctggacca aggcgggtat gagcgctact ttcttgcgct
    18481 caaccagtcg ggcggagcgt ccaaaattcc ggcaatcgcc gagaaagcca ttgccaactt
    18541 tcccaacaac ccggacctgc tggtcgtggc ggccaacacg gcgattgtca aaaaccaaaa
    18601 cgaccgggct ctggcgctcg ccactcgtct gttaaacgcg gctggcaagc agcagaagcc
    18661 cgaaggcgtc ccggccgccg aatgggaaaa gaagaaaagt ctggagatga ctacaggcca
    18721 ttacattgcg ggcctggcat ccgcagcaaa gggtcaatat tatcaagccg accagcattt
    18781 gcgcgctgcg cttccatccc tccaaggcac tgctatggcc ggtccggcgc tctactatct
    18841 gggcgtagcg aactataacc tggggaaaca gatgatgaac aaggctcggg tgcttgaagg
    18901 cgcaaagttc agcgaacaag cggccgccgt gaagggcttc ccactggccc aggaggccgc
    18961 gcacaattcg ctggtgatgc ggagcgaagc cggcacaatg cgctgaacga gatttcaagc
    19021 gcttcggcgt gctcaagagt acggggccgc aggggcgttt ccccgagccc acgccgatgt
    19081 cagctcgaat gtcatgacgg cgcggcgaat tctgcgaatt gggaatttta ttcgttgtaa
    19141 aacacgaata tggaatgtcc gatcgccgcc acccgcttgc tgagcggaag ccagcttctg
    19201 taatcccggc tgggctcggc ccggctggac tggtagagcg aggcagaaac ggcgatccag
    19261 ccctttcgtg ccgcatcggt aggttggacc gatcggtact tgctcccaag gtagtattcc
    19321 ggctccgctc ggccgaaata gtcaagattg atcacctcga tgcggttttg ctccacgaac
    19381 cgcgccagcc gcttcaaatc ctggccccag tcgagattgg aatcgaccac gtaccggtat
    19441 cctccctcgg gaccgcccgc cagttcattg aagtacgcaa tatagtgcgg atacacggca
    19501 gtcaccgaaa ccgcctgcca gataagcaac ccggcgaggc aagcggtggc aatcgatttg
    19561 gatcgcttgg atccggcgct tgtcaactgt cgcacggtac tggcggccgc aaggatgtac
    19621 accggtatga acgccggaag caagtgccgg acgccaatat tgagattgca gccaatcgat
    19681 gccagccaat aaacagcgac aatgatggca aacgccgctt cggcaaaatg cttgcgcacc
    19741 caatcgcccc gcttgggcgc gtccaggaac cgtcttacag caaaggcgac tgcaaaaagg
    19801 gtcagaatat gaaccgccag aggttccttt accgcataca caaacggaaa atagagaggc
    19861 gttctcgatc cggatacctg ccccaggaag tacttcgcat tgccatatgt cgcgcggccg
    19921 aaaatgaaca gcaacccaaa aagtatggcg cgtaggcacg aagcactggc ttgccggcca
    19981 taaaaattac aatgcccttg ggaaaatctc tgacggcatg ctcgattgac cgcggcagat
    20041 ggcgggaggc ca
//
LOCUS       DASREK010000260        24564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1451547, whole genome shotgun sequence.
ACCESSION   DASREK010000260 DASREK010000000
VERSION     DASREK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 24564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24564
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451547"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFA54_14090"
     CDS             complement(<1..257)
                     /locus_tag="VFA54_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51992.1"
                     /translation="MTNTLHRYGNSESFRDDYILFAISSRGNNDDGAIEKLKTFLRIC
                     ANHKPANMGNGDFGSYQPQRNLNPAAHWHRECRQDYQAVIDG"
     gene            complement(229..1083)
                     /locus_tag="VFA54_14095"
     CDS             complement(229..1083)
                     /locus_tag="VFA54_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZS51993.1"
                     /translation="MQIDPKKCVACGNCIPVCPMGAIYIDPAINRATINYDECVECSA
                     CYRGMSQEHLNPVMVRTVRSLAKLFRFRFDPEPDVCPTAAFVMEELEWPRIARRVFSD
                     PLVEHKSTGIHGRGTEEVKTNDVAHRVGLGEAGYVIEFGRPGVGVRFRDIEKMTRALA
                     ALGVNFEAKNPITHLMVDQSTGQLRPDLLNEKILSAIVEIKTKIEQVPDVLDVVQRVS
                     KQIDTVIALGVSTRCDANGEETILSRVLDEHGYTYERAKTNLGLGRVTNIKKLQETAH
                     DEHLASVR"
     gene            complement(1221..2519)
                     /locus_tag="VFA54_14100"
     CDS             complement(1221..2519)
                     /locus_tag="VFA54_14100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS51994.1"
                     /translation="MAASRVRHWVLLLTFLTSFIMYMDRVCMGSAAPSIMAEFHISKV
                     TMGGAASAFNWAYALFQVPGGWMADRFGPRIVLAGAMAWWSLFTMGTGFSFSASSLAV
                     TQWFFGMGEAAAFPAASRALVRWLPSSRRAFGQGFQHAGSRLGAAVTPPLVIYLIRNL
                     GWHWVFYIMGIVGIPWAVLWYLYYRNYPQQHRGVNEQELEILGAAGSAGKPKAAVKVP
                     WRRILHSSNLWALCSMYFCYGWVLWVYLFWFPTYLVEARHFSRAQLGLAASSPLIAAV
                     LTNVLGGWISDKLAHRWGDLRRGRIFVSILGFLLAGLMLIPGAMVSSVSAGLTCLTVA
                     MAGLELTVAVSWAICLDIGGDFAGSVSAMMNMLGNIGGALSPLVIGYLATRFGWNYAL
                     LLASGMCISAALIATRIDPNRSAVAAEAAEPAALTPQLTR"
     gene            2644..3369
                     /gene="gph"
                     /locus_tag="VFA54_14105"
     CDS             2644..3369
                     /gene="gph"
                     /locus_tag="VFA54_14105"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686950.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HZS51995.1"
                     /translation="MPLELLVFDLDGTLIDSKLDLALSVNATRADLKLPPLPHETIFS
                     YVGNGAPALIRRALGEGASEEQFQRSLQFFLDYYRAHMLENTRLYPGIRETLDRLAMA
                     GVQMAVLTNKPVRFSRDLIRGLGLADRFSAVYGGNSFDTKKPNPEGLRHLMAELHAAP
                     QETMVVGDSGIDVQTARSAGAWSCGVTYGFQPETFADNPPDFLVNTPAELLKIVGNAR
                     QGGLSGRPSELARPAGDGGLPAE"
     gene            3477..3935
                     /locus_tag="VFA54_14110"
     CDS             3477..3935
                     /locus_tag="VFA54_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51996.1"
                     /translation="MRVTLRIRTGPEKQIVRNRPPNRRQAHAAASFLWPLALFSFFVC
                     AWRWAYELGWLGHFLLSGGALCHWQIWFMAGAVSQTAAVRLGHYAARPLDAWAQRPQA
                     AAPAPEVPISRAPAQPFLPRGAELWSAALAPGRRDPAALAEELSDPVVHS"
     gene            complement(3912..4541)
                     /locus_tag="VFA54_14115"
     CDS             complement(3912..4541)
                     /locus_tag="VFA54_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS51997.1"
                     /translation="MRRHHFSIHPRNIVWEIIDHIRGREQMLVRSAEELPLIVISYPR
                     GNLAYAESLKYSITRTYKLLSPATRQRYTPVMPHLPSLVVVVLRARNPCSCLGHHHPV
                     GTQSRMGRRLASDTGLPVGEIDLAVEAIREWEPLPLAALAAEPAAGDRRELEQFRFHV
                     ALLAVFLHELEHLAFPDHGEQQVRQRSTEFYAASLREFFSEELGVNYGI"
     gene            4732..5502
                     /locus_tag="VFA54_14120"
     CDS             4732..5502
                     /locus_tag="VFA54_14120"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZS51998.1"
                     /translation="MGTYRSRYPGSSYGIGGYFPRGVKWLLIINTAVFVFAFILGLID
                     PVMAGSFLGIFGLRAATFLRGAFWQPVTYMFLHDPTGFGHILINMLTLWMFGIDLERD
                     WGTPRFLKYYFVCGIGAGICDIVVSLVLSSAGAPTIGASGAIYGVLLAFGLLYPNRTV
                     FFSFLFPIPAKYFVMIMGAIAFLSSFNRASPVSNVAHLGGMLFGYLYLRFGLHRVQWD
                     AGRITDIYHRWRRRRLQQKFKVYMRKREQEQDRDRWVH"
     gene            5664..6803
                     /locus_tag="VFA54_14125"
     CDS             5664..6803
                     /locus_tag="VFA54_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS51999.1"
                     /translation="MRTTNRFRNAAGLGLFLLLGFSLLAQQEGPLPSAQGPERDAGES
                     VARPKKKSEPPPAARPKRPPIPESKDMPTFKSESNLVTVDVAVLDSRGNFIPNIPREK
                     FRILEDNVPQKIVNFGPSEAPMTVCMVIEFSNLFQQFWTESWYQTLSASYGFLQTLRP
                     EDWVAVVAYDLKPEILSDFSQDKSKAYDAMRRLQIAAFSESNLYDALADTIDRMKDIE
                     GRKAIVLISSGIDTFSKLTFDKIRRIVQTGGTAIYAIGLMQALREWYDAAGMMGPIQR
                     LNFLEADNQMNTFARESGGMAFFPRFYGEFPTIFQQISASLRNQYRLTYEPSNAAHDG
                     KFRKIKVQLIDPQTNKELRIVDQKGKNIKYQILAKAGYIAPREVE"
     gene            6947..7426
                     /locus_tag="VFA54_14130"
     CDS             6947..7426
                     /locus_tag="VFA54_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS52000.1"
                     /translation="MRPLSLRILLIDDNRHGLIARRAVLEQQGHKVVIASSGRDGLDQ
                     FQSQKFDIVVTDYRMPELSGQQVIRTIRQSQPKTAIVLLSGYVDALGLTEQSTGADIV
                     LAKGPGEVQELLRAVARLAKRRPAPSKPPGKAPETRRKSDLGQRSVRPRSRNKSSVR"
     gene            7431..8138
                     /locus_tag="VFA54_14135"
     CDS             7431..8138
                     /locus_tag="VFA54_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZS52001.1"
                     /translation="MPALKITWLGHGTFQMEAGLGEIFLIDPWTDGNPAYPRSHAVSK
                     LDAMLITHGHFDHIGDAVSLAKRVRPKKVVGIYELCHWLEGKGVENLSPMNKGGTQAV
                     GSLRVTMTHADHSCGILDDGRIVYGGEACGYVIALPDGRHLYHAGDTNVFGDMAIIRE
                     LYNPELVLLPIGDHFTMGPREAAHACRLLRPKMVIPMHFGTFPLLSGRPEQLRDQLRD
                     MPEVEVLALAPGQTITW"
     gene            complement(8305..9000)
                     /gene="larB"
                     /locus_tag="VFA54_14140"
     CDS             complement(8305..9000)
                     /gene="larB"
                     /locus_tag="VFA54_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZS52002.1"
                     /translation="MPVETAIQRLRHLPFEDLGFAKVDHHRALRHGMPEVILASGKSP
                     KQIAAISRQLLKQNKNLLITRANKEVYGVVKKVARAAEFFPLSGAIRVHGDRTILGKG
                     KIAVVCAGTSDIPVAEEAQVTAEVMGNEVETLYDVGVAGIHRLINNRERLTQARVIVC
                     CAGMEGALPSVVGGLVSVPVIAVPTSVGYGASLHGLAALLGMLNSCASNVTVVNIDNG
                     FGAAYVASMINRL"
     gene            complement(9367..9840)
                     /locus_tag="VFA54_14145"
     CDS             complement(9367..9840)
                     /locus_tag="VFA54_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZS52003.1"
                     /translation="MRRSILPVVMCLVSLAACSSSPSEGKGDESETQLTTMPVTLPNG
                     KTIRAELATDPASQERGLMYRTRLAPDRGMLFVFGEAGNYPFWMYHTLIPLDIIWMDS
                     NRTIVYISANTPPCRSEKAEECPNYGGAERAQFVLELAAGQAAANGLKTGDRLRF"
     gene            complement(9932..11290)
                     /locus_tag="VFA54_14150"
     CDS             complement(9932..11290)
                     /locus_tag="VFA54_14150"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZS52004.1"
                     /translation="MPRAFLLCLIATALLAAQNQPQRGSSRRKSTTGSSSTEASTAPG
                     RWPLAAIHVSGNRIFPTEKIVQAAGLRLGSLVNASDFEAALRRLTGTGAFETVAFHYE
                     PVDKQLAVTFEVREVVDLYPVAFEGLGAPDEELRKAIEEKVPLFGPQVPATGIMVQHI
                     LQAIQDYLAAKHQSAELTSGLLPGAGGKLAMTFRSAAPAPKIVYVRFENTTVIPAETL
                     QKAFVPNSVGVPYTEKRLAELLDVNIQPMFEEKGRLEARFGPFRTEKSTEPEGVIVTV
                     PVQEGDEFHIDHIKLEGNERLSTAELRPVLKLASGDLANFTLIHKAAGDLERRYQHEG
                     YIQARAAVERTLDGKKKTVDLTFRISEGEVYTMRNLTIRGLDINAEAAVKRMWSLKKG
                     QPFNGEYPEFFLQRIEEEGLFDNLGGTSHRESVDEQAKTVDVVLEFKGGAGAPQRGRR
                     RP"
     gene            11482..12843
                     /locus_tag="VFA54_14155"
     CDS             11482..12843
                     /locus_tag="VFA54_14155"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZS52005.1"
                     /translation="MALSLRRLFAFLTALLPAGGLWLFAPMAAAQDRAAAVFHSEPQS
                     RNLRSFDYIWTTIRDKHWDPKLGGLDWQSVRDELRPRVEKTATPAEARPILQEMIGRL
                     HQSHFGVVPSDIYQDIQEPSGQIAPEDEGWTGLDVRVIGGKALVTSVDAGAPAYIRGV
                     RPGWQLLKIDHAPVESLLEKVNTAYWNSTMRGLLLARAVLSKLNGRVGTQARIEFLDG
                     AGKIVETDIARTRPSGIQSRFGYLPACYVRMESRRLGGNIGYIGFNFFLAPAAVMKKF
                     EEAVKSCRECPGIIIDLRGNPGGIGAMAIGMAGWFINQPNQQLGAMYTRDATLHFIVN
                     PRLETYGGPLAILVDDCSASTSEVFAQGMRDLGRARIFGIKTAGAALPSVFERLPNGD
                     GFQYAIANYLSKGGKTLEGAGVVPDEEVKLSRAALLEGRDPVLEAALRWIQAQSTQNG
                     SGR"
     gene            12846..13625
                     /locus_tag="VFA54_14160"
     CDS             12846..13625
                     /locus_tag="VFA54_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52006.1"
                     /translation="MIAKKASRFLIAALTIEWALAGGSSRAQEKLPAAETVLDQYVEV
                     TGGREAYKKIHTQITIATMEFVGKGIKATLTSYKAIPHNSYNVAEMQGIGKIEQGTSG
                     DIAWERSPLQGPRVKSGEERASALRDAALNAEWRELFKKAEMEGVENVGSRPCYKILL
                     TPAEGSPETRYYDTKTHLLVKVNKVVKSQMGEIPAEVTLSDYRDAGGILVPHRVLQKG
                     LGQEFLITIEKIEQNPEIPASRFDLPADVKALLEKSGAGGN"
     gene            13667..15427
                     /locus_tag="VFA54_14165"
     CDS             13667..15427
                     /locus_tag="VFA54_14165"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HZS52007.1"
                     /translation="MNEFFQQIGTFLQANVAGRELNDEVRSEAARTGPQPPAGRAYYL
                     SLTRDPVAADIHYVAVDLDGSWLLRSTPGGVDALPFSSADWSVIRRFAENIDPSFLPR
                     PQKDQPAVAVGNRHPEISLPAAFDAFGRILRSKCVNMASTVQLSATREMTTAARLAGR
                     TGENPTEVGHTRVSIEHLYHAGLWAAIRAGWRHGYNAEADHFIISGASGAEIAHSIEQ
                     TKAAIRAAAGYTKFTTDTSRLFRLEADPRHPHAWSDAQVEQEFERILNREQQRWVLSE
                     FGRSFSIEGRVYSFTPAEIRRLAVKFSQSLQLNEDLYDYIQQAKRESGRSTDFDFEPS
                     IDEAETLTTAREMIFYMHWLASRGRRAQLVPPNLGFKKRQAYPETAAALREYAAHKMW
                     PELLPRVEREFGGDPVKELGARVRELADVARYFNGTLSIHSGSGKQARVLQMIGRSTG
                     GRVNYKISGELQLQLLDVLSEQPAGSPWRSLFERMVDRCVQFAEAGAFGEESQLARIY
                     KEKGLGYYLGDRLKGRVDGNLFLVFWLGNLVGSRDAADPSGDHRFFKEKLDELPAALV
                     QEVQRRNSDYIVWLAEQLRP"
     gene            complement(15483..16649)
                     /locus_tag="VFA54_14170"
     CDS             complement(15483..16649)
                     /locus_tag="VFA54_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52008.1"
                     /translation="MRLLALLFAGLALSALAQTPPAEILVRSTFEGIPALVLSNDKLE
                     LTILSQGGAFANLALRDDPEKLSPYWNPRRMAREDGRRPPSGFEAGHFVCVDGFGPVS
                     EQERAAGLPGHGEANKQAWRVVAAARSGKTASVTFSVDLPLVEERFTRTVRLVDGENV
                     VYVESGLESLLAFDRPICWAEHATIGSPFLEPGKTAVDLRAVRAKTRPHDPKPGPLPH
                     RLASSLEFTWPMAPDGSGQLIDLRAAPPNPNSGDHTTALMDMNRKWAFVTALHPEKRL
                     LVGYLFHPQEYPWVQNWENYPPTLKMARGFEFGTQPFDVPRREVIDLRSMFDAPVYRW
                     LPAKSKIESRFLFFYTRTPEGFQRVDEVSLEQGVIRIEDRTAGKSITLSASLEP"
     gene            16678..18063
                     /locus_tag="VFA54_14175"
     CDS             16678..18063
                     /locus_tag="VFA54_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52009.1"
                     /translation="MPPRKITRAFGPVIGFLFLLRPYALAQDSHPSELHDPAVIQYFR
                     QGGHQRLAHFTTLLRHPVEASQDLLLVWGGVQAPSWTGAGLWWNQTDVMGLFLQEREH
                     PQQVHLLALLLNDDQSCAVEVARIAPGELVLWRTGEKGGIFDSIKFFFDARSRRIFRR
                     IEYAPFTVRRILMKDGVLLFLAGGGNRLLVIRLLREAPAGRIPFQVLSEKEAAPLLAE
                     APLIVSSTASGEFGDLRRAPASLRFGPGKRFALEPETTQSWGTIWRTVTERAGGRAAS
                     FMLPQSSCVEYVQARREAMRRTFPVIPAGTICQGSGPEILETIGAWEIAGDGRLWLGK
                     TFYDAEGSVGVGGFGYFDPGDREFHIFSPPEVRDWSVSALRVGSDAVWLGLMHRGEYG
                     DSPGGLLRWDRSSHAINAYSVSSIVHDIALSGSRIYLAAASGIFVLENGRLQQYIVDV
                     GPDGGYEVVPR"
     gene            18192..19862
                     /locus_tag="VFA54_14180"
     CDS             18192..19862
                     /locus_tag="VFA54_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52010.1"
                     /translation="MSRHPRGKSKKRLHPKVENFVEAAPSPASHASLPEHRRSADEPR
                     ETSILRIETLIWAGALLVIYACVATWGQFDFSDLMGYYNMQADAFLAGHLYIARNPSE
                     GYLMDMVPFEGRFYLQWGPFPAVLHMMAKLAGGKLTDRVACILAGWLTSLVFLKIVVV
                     LRGRYFPALPKWLCCWFFFTFALATPTAIVALRGTIYHESIAWAALLVLFAFWALLEY
                     LDRQSAPWVFLAGAGAGLAILTRVSLGLYAAGLLAGVAVLQRHWGRRKKVAAMHLAAL
                     AAPAAAGVLLMMAYNQARFRSPWEYGLKYLPVAAADKPAYALNRVPENFLHYVLAPIH
                     VTLDCPWFTHKGWQPLVKTERAEDMSSMFLASPFLLLGFLAWPLFRTRDGRIFPLKVF
                     AAVAGLSAFAVFFSLLCFVGTSRRYMQDFVPMLMVLALLGVAYSAKTGSDWRRWQAPA
                     WGIFALSALLHVHLVFFQPETWAPLDPNVSKAFVALGPLVRRVLPGHQLDSRLAIHHN
                     DLGLLYMDQGRYAEAIPHFEQANRYMPASVSIQHNLTYARRLQAASGR"
     gene            20093..22129
                     /locus_tag="VFA54_14185"
     CDS             20093..22129
                     /locus_tag="VFA54_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS52011.1"
                     /translation="MGARRMLGRRAACLLIWTFLESAALAECIRVSVRDPSGLAAAGA
                     AVHVDGGSANLTDDAGNAVICGLAAGRHKITVEKAEFATLSLVANAPGEQTITLALEP
                     RVESPVVVTGTPEPAQLAEVDRSIIVIPVENAVVPATSLAQLLKTDSTVDVQERGIDG
                     TQADVGIRGGSFSQTLVLINGMRVNDAQSGHHDMDLPLPLESVSRMEVLHGSGSTLYG
                     SDAVGGAVNVITRRPEATELNVSSGIGDFGWNREALSGGFRFGKWSQQAAVARDFSTG
                     FRPGRDFRNLAVASQSFFDESFGSTSLLFAYNDRPFGANGFYGSFPSWEETGTKLVAL
                     SQTVGEHRVQFSYRRHTDHYVLYRDQPEIFQNFHTSGTWYGSYAFHHALSGRVDLSAG
                     ADGLSEAIESTNLSDHRRERASGFFVLQARPGPRLSLSAGLREEIYRKWSAVASPTLA
                     AGVRLGKGWKARASLSRTFRVPTYTELYYHDPANIGNPNLRPESAWSYEAGLDWWGRG
                     GTNISATWFERRERNTIDFVRPAGASVWQAMNFERLNFHGGEIGARDRIAVRGRSADV
                     GISYTIIRASREIIPGVASLYIFNFPRNSVAFTAAAPLGRYAVVRTRVGAFDRSWQPA
                     VALWDISVAGQGSRFQPFLQITNLANAYYESFPGLAQPGRWIRGGMKIELLGKR"
     gene            22254..23648
                     /gene="selA"
                     /locus_tag="VFA54_14190"
     CDS             22254..23648
                     /gene="selA"
                     /locus_tag="VFA54_14190"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZS52012.1"
                     /translation="MPASAENDFRVLPSVDEVLKQEAIARLTERFPRHMVVEAVREVL
                     GTFRDKLVEGRREEIEYQLPQLPETVRQRLDDLISPSLRSVINATGVVLHTNLGRAPL
                     SASALRAIAGTAGAYNNLEYDLTKGARGRRDVHAAALLERLLGAPAIVVNNNAAAIFL
                     ALHELASGGEVIVSRGELIEIGEGFRIPDILARSGARLREVGTTNRTRIEDYRAAITG
                     ETRAILRVHHSNFRIVGFTDRPSLKDLVDAGSQAGIPVVEDLGSGCLVDLKGYGLEHE
                     PTVGESIAAGIDVVTFSGDKLLGGPQAGILAGKKEWIARIRRNPLFRALRVDKLIYAA
                     LSATLRDYLLEDRAGIPIYAMLDQTADQIRERAEKLVAALPGIEADLCPGSSVIGGGS
                     TPAMELPTTLILLRPPEAESAAACERLLRTGATPVVARLEKQRIILDLRTVLPEQEVL
                     LAGAIRSAWSRNGF"
     gene            complement(23675..>24564)
                     /locus_tag="VFA54_14195"
     CDS             complement(23675..>24564)
                     /locus_tag="VFA54_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HZS52013.1"
                     /translation="VTMGTSRAFANGGSRLFEFDRNGKFIREIGHGLYGFVFAHAVRV
                     DPQDNIWVVDEGSNMVIKFNPEGRVVMTMGRKAEELRVPSVPTVPPPQAAANRPAAAA
                     APALPAGAGAQTDVFNRPTDVAWDAAGNIFVSDGYGNSRVAKFDKNGRFLKSWGSRGT
                     APGQFNTPHTIATDAQGNVYVGDRGNRRIQVFDNSGEFKTLYLNVGAPWAVCISPGAH
                     QYLFSSNSNGTEDMENGEIYKMELDGRILGKFGKAGKLLKEFGSVHEIDCRNPNELYV
                     GEITNWRVQRLTLHPIAAN"
BASE COUNT         4628 a         7519 c         7535 g         4882 t
ORIGIN      
        1 ccatcgatta cggcctggta atcctgccgg cactcgcgat gccaatgcgc cgccggattg
       61 agatttctct gtggctgata gctgccgaag tccccgttgc ccatgttcgc cggcttgtgg
      121 ttggcgcaaa tgcgcagaaa cgtcttcagc ttctcaatgg cgccgtcgtc attgttaccc
      181 cgggaggaga tcgcgaacag gatgtagtcg tcccggaagc tctccgagtt accgtaccga
      241 tgcaaggtgt tcgtcatgag ccgtctcctg gagcttttta atattcgtca cgcggccgag
      301 gccgaggttt gtcttggcgc gctcgtaagt gtatccgtgc tcatcgagca ccctggaaag
      361 gatcgtctcc tcgccgttgg cgtcgcagcg cgtggaaact ccgagcgcga tcaccgtgtc
      421 gatttgcttg gatacgcgct gcacgacgtc gagcacgtcc ggcacctgct cgattttcgt
      481 tttaatctca acgatcgcgg agagaatttt ttcattgagc aggtcaggcc gcaactgtcc
      541 cgtgctttgg tccaccatca aatgggtgat cggattcttg gcttcaaaat tgacgcccaa
      601 agcggccaga gccctggtca ttttctcgat gtcgcggaag cgcacgccca caccggggcg
      661 gccgaactca atcacgtagc cggcctcgcc cagccccacg cggtgggcca catcgttggt
      721 cttcacttcc tcagtgccgc gcccgtggat tccggtggac ttgtgctcga ccaggggatc
      781 ggaaaaaacg cgtcgcgcaa tgcggggcca ctcaagctcc tccattacga acgcagcagt
      841 ggggcagaca tccggctcgg gatcgaaccg gaagcgaaac agcttcgcca ggctgcgcac
      901 cgtgcgcacc ataaccggat tcaggtgctc ctggctcatg ccccggtagc aagccgaaca
      961 ctccacacac tcgtcgtaat tgatcgtggc gcggttgata gccggatcga tgtagattgc
     1021 ccccatgggg catacaggga tgcagttacc acaagctacg cacttcttcg ggtcaatttg
     1081 catcgcgcac ttccttagaa actacctgcc gccggtagtt taacacattc gccggcccgc
     1141 ggattaccgc cggcggcaaa tacgcctggc cgctgcactg tcgaaacgcg ggacgcgccc
     1201 ggcagagcca cggctccggc tcacctggtg agctgcggtg tgagcgccgc tggctcagcg
     1261 gcttccgccg ccacggccga ccgattcgga tcgattcggg tcgcgatcaa ggccgctgaa
     1321 atacacattc cactcgcgag caaaagagca tagttccagc caaaacgggt cgccaggtat
     1381 ccgatcacca gcggcgaaag cgcgccgccg atgttcccga gcatgttcat catggccgaa
     1441 acggaacccg cgaagtcccc gccgatatcc aggcagatcg cccaggagac ggccaccgtc
     1501 agctcgagcc cggccatggc taccgtgagg cacgtcaagc cggctgagac gctggaaacc
     1561 atcgcgcccg gaatcagcat caagcccgcc agcagaaaac ctagaatcga tacgaaaatt
     1621 cgaccgcggc gcaggtcgcc ccaccggtgc gcgagtttgt ctgaaatcca tccgccgagg
     1681 acgttggtca agaccgcggc gataagcggg ctgctcgcgg ccaggccgag ttgcgcgcgc
     1741 gagaagtgcc gcgcctccac caagtaggtg gggaaccaaa aaagatagac ccaaagcacc
     1801 cagccatagc aaaagtacat ggagcacagc gcccacaaat tactgctgtg caggatacgg
     1861 cgccagggga ccttgaccgc tgctttgggc tttcccgcag agccagccgc cccaagtatt
     1921 tcgagctcct gctcatttac gccccgatgc tgctgcggat aattccggta gtagagatac
     1981 caaaggaccg cccagggaat gcccacgatt cccatgatgt aaaacaccca atgccagcct
     2041 agattccgga tcagataaat caccaggggc ggagtaacgg cggcgccgag acgcgacccg
     2101 gcgtgctgga acccttggcc gaacgcacgc cggctactgg gcagccagcg gaccagcgcc
     2161 cgggaagccg ccggaaaggc agccgcttcg cccatgccga aaaaccactg ggtgaccgcc
     2221 agtgagctgg cgctaaagga aaatcctgtg cccatggtaa aaagcgacca ccaggccatg
     2281 gcgcctgcca atacgatacg cggcccgaaa cggtcggcca tccagccgcc cggtacttga
     2341 aacagagcat aggcccaatt gaacgctgaa gccgcgccgc ccatcgtcac tttactgata
     2401 tgaaattcgg ccatgatgga cggcgccgcc gaacccatgc agacgcggtc catgtacatg
     2461 atgaaagacg tcagaaaagt cagtagtaaa acccaatgac gaacccggct ggcagccatg
     2521 ttgcgcatta taacgaagag aagagcaaaa acagaattca tcgcccggtc aaaatgcgcc
     2581 cgcgcatcgg ccgttccgga tagcggacgt gttcgatccc gtgattgcta aactggacag
     2641 gagatgccac tcgagttgct ggtcttcgac ctcgatggaa cattgattga ttccaagctg
     2701 gatctggcgc tttccgtaaa tgccacgcgg gccgatctga agctgcctcc cctcccccac
     2761 gaaaccattt tttcctacgt cggcaacggc gctccggcgc ttatccggcg agctctcggc
     2821 gaaggggcga gtgaagaaca gttccaacgc tcgctgcaat tttttctgga ctactaccgc
     2881 gcccacatgc tggagaacac gcgcctgtat cccggaatcc gggagactct ggaccgcctg
     2941 gcgatggcag gtgtgcagat ggccgtcctt accaacaagc cggttcgctt cagccgcgac
     3001 ctgattcgcg gactcgggtt ggccgaccgt ttcagcgcgg tctacggcgg caacagtttc
     3061 gacaccaaaa agccgaatcc ggaggggctg cggcacctca tggccgagct ccacgccgct
     3121 ccgcaggaaa caatggtcgt gggggatagc ggcattgacg tgcagacggc tcgcagcgcc
     3181 ggggcatggt cctgcggagt gacctatgga ttccagcccg agactttcgc cgacaacccg
     3241 ccggactttc tggtgaacac ccccgccgag ctgctgaaga ttgtgggaaa cgcccggcaa
     3301 ggtggattat ccggcaggcc ttccgaactg gcgcgccctg cgggcgacgg cggactgccg
     3361 gccgaataag gttttaagcc cgaggtcgta ttgccggggc cagcgccgct gcatccagcg
     3421 gtcgcattgc cgggcggcgg ggaaatgtta cactggttcg aacggaaccc gcaggtatgc
     3481 gagtcaccct tcgaatccga acgggcccgg aaaagcagat cgtgcgtaac cggcctccga
     3541 atcgcaggca ggctcacgcg gcggcttcct tcctgtggcc gcttgctctc ttttcatttt
     3601 tcgtttgcgc ctggcggtgg gcctacgagt tgggatggct gggccatttc ttgctctccg
     3661 ggggcgcttt gtgccactgg caaatctggt ttatggcggg cgccgtatcg cagactgccg
     3721 ccgtacgcct gggtcattat gcggctcgcc cgctggatgc ctgggcgcag cgtccgcagg
     3781 ctgcggctcc ggctcccgag gtcccaatct cccgcgcccc ggctcagccc ttcttgcccc
     3841 gcggcgcgga gttgtggtct gccgcgctgg cccccgggcg gcgtgatcct gccgcgctgg
     3901 ccgaagaatt gtcagatccc gtagttcact cctaactctt ccgagaaaaa ttcacgcagg
     3961 gacgcggcgt aaaactcggt gctgcgctgg cggacctgct gttctccgtg atccgggaag
     4021 gcaagatgct ccagctcgtg caggaacacg gccaggagag ctacatggaa ccggaactgc
     4081 tcgagctcgc gccggtcgcc ggccgcaggc tctgccgcca acgcggccaa gggcagaggt
     4141 tcccattcgc ggatcgcctc gacggccaaa tcgatttcgc cgaccggaag ccccgtatcg
     4201 ctggcgagcc gccggcccat gcggctttgc gtgcccaccg gatgatgatg gcccaggcag
     4261 ctacagggat tccgcgcgcg caacacaacg accaccaggg agggcaggtg aggcatcacc
     4321 ggcgtgtagc gctgccgcgt agccggagag agcagcttgt atgtgcgggt gatggaatat
     4381 ttcagggact ctgcgtaagc cagattcccg cgaggatagc tgattacaat gagcggcaac
     4441 tcttcggcgg accgcacaag catctgctcg cgcccgcgga tgtggtcgat aatttcccat
     4501 acaatgttgc ggggatgaat cgaaaaatga tggcgccgca taccgtcggt cccatcataa
     4561 cgcgcccttg tttcctgcgc tcgaaactgc ccggctccgg cggcgctgct gcgggcggga
     4621 ccgagtcttt gaatacaccg catcttcgaa catcagaccg gcaggcgtgg ccaatgcaaa
     4681 gctgctattc tgctaaggag agtcggtctt caacgccggt tcacctgctc tatgggcacc
     4741 tatcgcagcc gctatccagg cagcagctat ggaatcggag gatattttcc gcggggcgtc
     4801 aaatggctgc tgatcatcaa taccgccgtc ttcgtttttg ccttcattct gggcctcatc
     4861 gatccggtga tggctgggtc cttcctgggc atcttcggtt tgagggccgc tacatttctg
     4921 cggggggcct tctggcagcc ggtcacgtac atgtttctgc acgaccccac cggtttcggg
     4981 cacatcctga tcaacatgct gacgttatgg atgtttggga tcgatctgga gcgggattgg
     5041 ggcactccgc gctttctcaa gtactatttc gtatgcggaa ttggagccgg catctgcgac
     5101 atcgtggtga gcctcgtgct ctcctcggcc ggcgctccga cgatcggcgc ctcgggagcc
     5161 atctatggag ttcttctggc gttcggacta ctgtacccaa accggacggt cttcttcagc
     5221 tttttgtttc cgatcccggc caagtacttt gtcatgatca tgggcgccat cgctttcctt
     5281 tccagcttca accgggctag cccggtaagt aatgtggcgc acctgggcgg aatgctattc
     5341 ggctacttgt atctccgttt cgggctgcac cgggtgcagt gggatgccgg ccggatcacc
     5401 gacatctacc accggtggcg gcgtcgcagg ctccagcaga aattcaaagt ttacatgcgc
     5461 aaacgcgagc aagaacagga tcgggaccgt tgggttcact aatccgcagg cgcttcacag
     5521 aacggagccg ctcccggccg ctgcggtgcg cggaaccacc gctttgccgg cgagatttgg
     5581 cttcgtgact tcccgccccg ggctgtacta cgataggggt aaccggcaag gttggccgtc
     5641 agtcctttta ttggcaggtc gtcatgagaa caactaaccg atttcgaaat gccgccgggc
     5701 taggtttgtt tttgctcctg ggtttcagct tgctggcgca gcaggagggc cctctgccga
     5761 gcgcccaagg tccggagcgg gatgccggcg agtccgtggc tcggccgaaa aagaaatccg
     5821 agccgccgcc ggcggcgcgc ccgaagcgcc cgcccattcc cgaatccaag gacatgccga
     5881 ccttcaagag cgagtcgaat ctggttacgg tcgatgtcgc tgtgcttgac agccgcggaa
     5941 acttcattcc gaatattccg cgcgagaagt ttcgcatcct ggaagacaac gtgccccaga
     6001 agatcgtgaa cttcggcccc agcgaagcgc cgatgacggt ctgcatggtc attgagttca
     6061 gcaacctctt ccagcaattc tggacggagt cgtggtacca gacgctctcc gcttcctacg
     6121 gctttttaca aacgcttcgg cccgaggact gggtggcggt ggtagcttac gatctcaaac
     6181 ccgagattct gtccgacttt tctcaggaca agagcaaagc ctatgacgcg atgcgccggc
     6241 tccagatcgc ggccttttcc gagtcgaatc tttatgatgc cctggcggac actatcgatc
     6301 ggatgaagga cattgaaggc cgcaaggcca ttgtcttgat ctcgtccggc atcgacacgt
     6361 ttagcaaatt gacgttcgac aagattcgcc gcatcgtgca gaccggcggc accgcaatct
     6421 acgcaatcgg cctgatgcag gccttgcggg agtggtatga cgcggcgggc atgatgggac
     6481 cgattcagcg cttgaatttt ctcgaggcgg acaaccagat gaataccttc gccagagaat
     6541 ccggaggcat ggcgttcttc ccccggtttt acggtgagtt tcccacgatc tttcagcaaa
     6601 tatcggcctc gctgcggaac cagtaccggc tgacttacga gccgagcaac gccgcgcacg
     6661 atgggaagtt ccgcaaaatc aaggtccaac ttatcgatcc gcagactaac aaggagctgc
     6721 gcattgtgga ccagaagggc aagaacatca agtaccagat cctggcgaaa gcgggttaca
     6781 tcgccccgcg ggaagtagaa tagcggcggc gcgcgaaggc gccgcttcca atcacatttc
     6841 tgatctcagg tcaagcattc gatctttcct gttttcacgg cgataaaaat ctgttgacac
     6901 tctggaaatc gctctgatac gctacccgcg tttttcgttg cgtcgaatgc gcccgttaag
     6961 cctgcggatc ctcctgatcg acgacaaccg gcacggactg atcgcgcgcc gcgcggtgct
     7021 cgaacaacaa gggcacaaag tggtaatcgc ctccagcggg cgggacggtc ttgatcaatt
     7081 tcagtctcaa aaatttgaca tcgtagttac cgattaccgc atgccggagc tttcgggcca
     7141 gcaagtcatt cgcacgatcc gccagtcgca gcccaaaacc gccatcgttt tgctgtcggg
     7201 ctacgttgac gccctcggcc tgaccgagca atcgacggga gccgatatcg tgctggccaa
     7261 agggccgggc gaggtgcagg aattgttgcg cgccgtcgcg cgcctggcga aacggcggcc
     7321 agcgccttcc aagccacccg gcaaagcgcc ggagacgcga aggaagtcgg acctgggcca
     7381 gcgaagcgtc cggccgcgct ccagaaacaa gagcagcgtg aggtgatccg gtgccggcgt
     7441 tgaaaattac gtggctcggc cacggaactt ttcaaatgga agcgggcctg ggcgaaatct
     7501 ttctgatcga cccgtggacc gatgggaatc ccgcctaccc gcgctctcat gcggtctcga
     7561 agctcgacgc catgctgatc acgcatggcc actttgacca tatcggggat gctgtctcgc
     7621 tcgccaaacg ggttcggccc aagaaggtcg tcgggattta cgaattgtgc cattggctgg
     7681 agggcaaagg cgtggagaat ttgtccccca tgaacaaggg cggcacgcag gcagtgggtt
     7741 ccctcagggt caccatgacc cacgccgatc atagctgcgg gattctggac gatggccgga
     7801 ttgtttatgg cggcgaggct tgcggctatg tgattgcgct gccggacggc cggcatctgt
     7861 atcacgccgg agatacgaat gtcttcggag acatggcgat catacgggag ctgtataatc
     7921 cggagctcgt tttgcttccc atcggcgatc acttcaccat gggacctcgc gaggccgcgc
     7981 acgcctgccg actcctacgg ccgaaaatgg tgatccccat gcatttcggc acgttcccgc
     8041 ttctgtcggg ccggccggag caactccgag accagttgcg agacatgccg gaagtcgaag
     8101 tgctggccct tgcgcccggg caaacgatca cctggtagtc catcggggcg gcccggccgg
     8161 atccaagccc gcctcccgcc gacttgccat attctaagga agcggggagt gcgcttcgcg
     8221 ctcgcgccgg acgaaagcgg caggtcctgg gctgcggcag cggccgcctc ggtgagttct
     8281 ccaggttgac ttgaaatctc ctgtttacag ccggttgatc atgctggcta cgtaggcggc
     8341 gccaaagccg ttgtcgatat tcacgacggt tacattgctg gcgcaactgt tgagcatgcc
     8401 cagcagggcg gccaagccgt gcaggctggc gccatagcct acgctagtag gtaccgcgat
     8461 cacgggcacg gagaccagtc ctccgacaac gctcggcagt gcgccctcca tgccggcgca
     8521 gcagacgatc acgcgcgctt gcgtgagccg ttcccgattg ttgatgagcc tgtgaatgcc
     8581 ggcgaccccg acgtcataaa gcgtttccac ttcgttgccc atgacctcgg cggtcacctg
     8641 cgcttcctcc gccacaggaa tgtcgctggt cccggcgcaa accacggcga tttttccttt
     8701 gcccagaatg gttcggtcgc catgaacccg gatcgccccc gagagcggaa agaactccgc
     8761 cgcgcgcgcc accttcttca caaccccgta aacttcttta ttcgctcggg tgatcaacag
     8821 gttcttgttc tgcttgagta gctgccggga aatcgccgca atctgcttcg gcgatttgcc
     8881 ggaggccaaa atcacctcgg gcatgccgtg ccgcagcgcg cgatggtgat cgactttcgc
     8941 aaaccccaga tcttcaaagg gcaaatgccg caagcgctgg atggctgttt ccactggcat
     9001 cccgccactg cggacttgct gcaacagtgc ccgtagcttt tgttcggtca taatgcgctt
     9061 gtgtgtggcc ggctccgcgg atcaaaaccg ggccgccatg cggccgttta tgctcccggt
     9121 ccggcgagag atggatccga aaatcgtttt ccccggcgac gcctccccgt ttcctgggca
     9181 aagcaacctc gccctccaag ggagccgtga cttgctccgg ccgcttcacg ccgagcccgc
     9241 ggccccacat tctctagtcg ccgacgtgcg gcatcgcgtc gttccggccg gctacaagct
     9301 gtgagccgct gtccgtcgcc ggagaaaacg gggtcaggct ctccctgcgc ttgacaaccc
     9361 gccccgtcag aatcgcagac ggtcgccggt ctttagacca ttcgcggccg cctgcccggc
     9421 tgccagttcc agcacaaact gcgctcgttc cgccccgccg tagttcgggc actcctcagc
     9481 cttctcgctc cggcagggag gggtattggc ggaaatatac acgatagtcc ggttggaatc
     9541 catccagata atatcaagag ggatcagcgt gtgatacatc cagaatggat aattaccggc
     9601 ctctccgaac acgaaaagca tgccgcgatc cggcgccagt ctcgtgcggt acatcaaccc
     9661 gcgctcctga ctggcaggat ccgttgccag ctcagcacgg attgtctttc cattgggcag
     9721 cgtgaccggc attgtagtga gttgcgtctc gctttcgtcc cccttgcctt cactgggact
     9781 tgatgaacag gccgccagac tcacaaggca catgacaacc ggcaggatcg atctccgcat
     9841 gcgctgaatg ctgttgtacc tgtttccccg ggccggtaag cggggccgcc ggcccattcg
     9901 aaaacactcc cgctcgacat acggcctgcc gctaggggcg ccgccggccc cgctgcggag
     9961 ctcccgcgcc gcctttaaat tccaaaacca catccaccgt cttcgcctgc tcatcaacgc
    10021 tttcgcgatg cgaggttccg ccgaggttgt caaacaggcc ctcctcttcg atgcgctgca
    10081 agaagaattc aggatattct ccattgaagg gctggccttt cttgagagac cacatccttt
    10141 tgactgccgc ttccgcattg atatcgaggc ctctaatggt aaggttgcgc atcgtataca
    10201 cctcgccttc gctgatgcgg aaggtcaggt ccacggtctt ttttttgccg tcgagcgtcc
    10261 tttccactgc ggcccgggcc tggatgtagc cctcatgctg gtagcgccgc tcgaggtccc
    10321 cggcggcttt gtggatgaga gtgaaattgg cgaggtcgcc ggaagcgagt ttcagcacag
    10381 gacgcagctc ggcggtcgag aggcgctcgt ttccttccag cttgatgtgg tcaatatgaa
    10441 actcgtcgcc ttcctgcacc ggcaccgtta cgatcactcc ctccggctcg gtggatttct
    10501 cggtccggaa gggaccgaag cgcgcttcga gccgcccttt ttcctcgaac atcggctgga
    10561 tattgacgtc cagcagttcg gccagccgct tctccgtata ggggacgccg acgctgttgg
    10621 ggacaaaagc cttctgcaag gtctcggcgg gaatcaccgt ggtgttctcg aatctgacat
    10681 agacgatttt cggagccggg gcggccgacc ggaatgtcat cgccagcttg ccaccggccc
    10741 ccggcagcag gccgctggtg agttcggcgc tctgatgttt agccgccagg tagtcctgga
    10801 tagcttgcaa aatgtgctgc accatgatgc ctgtcgccgg aacttggggg ccgaacaggg
    10861 gaactttctc ctcgatcgcc tttctcagct cttcgtccgg cgccccgagc ccttcgaagg
    10921 cgaccgggta aaggtccacg acctcgcgga cttcgaatgt gaccgccagt tgcttatcca
    10981 ctggctcgta gtgaaaggcg acggtctcaa aggctcccgt gccggtcaaa cgccggaggg
    11041 cggcctcgaa atcggaagcg ttcacgagcg atcccaggcg gagacctgcc gcttgcacaa
    11101 tcttctccgt tggaaaaatc cgattgcccg aaacgtgaat cgccgccaga ggccatcggc
    11161 cgggggccgt ggaggcctct gtggatgaag aacccgttgt actttttcgc cgcgaagagc
    11221 cgcgctgcgg ctgattttgg gcagccagca gggcggttgc gatcaagcag agcagaaacg
    11281 cgcgcggcat ccgatgttcg tcctgtgtcc tataacgtag ggtaccagga acacaggaat
    11341 agggtcattg cctgcgcccg tgcaggagga tcgcctgcgc gggagcaaga agcgcctagt
    11401 gtagaatggc tttctgtggg gcgacttgat cgaagagctt ccgctgctgc ccggccgcct
    11461 ctccatttcg ggccccgctg tttggcactt tccctgcgca gactttttgc cttcctgacg
    11521 gcattgcttc ccgcgggcgg cttgtggctg tttgcgccga tggcagcggc tcaggatcgc
    11581 gcggcggcgg tcttccattc ggaaccacag tcgcggaatc ttcgatcctt cgactacatc
    11641 tggaccacga tccgcgataa gcactgggac ccgaagctag gcgggctgga ctggcaatcg
    11701 gtacgcgatg agctccggcc tagggtggaa aaaacggcga cgccagccga ggcgcggccg
    11761 attcttcaag agatgatcgg ccggctccat cagtctcatt tcggcgtcgt cccttctgat
    11821 atttatcagg acattcagga gccctccggc cagattgcgc cagaggatga gggatggacc
    11881 ggtctcgatg ttcgtgtcat cggcgggaag gcattggtga cttcggtcga tgccggcgcg
    11941 ccggcctaca tccgaggtgt ccgccccggc tggcagttgc taaaaatcga tcacgcgccg
    12001 gtggagtcgc tgctcgaaaa agtgaacacg gcgtactgga attcaaccat gcgcggcctg
    12061 ctgctggcgc gcgccgtgct ctccaagctc aacgggcgtg tgggcacgca ggctcgaatc
    12121 gaatttctgg acggcgccgg caaaattgtc gagaccgata tcgcccgcac gcggccgagc
    12181 ggaattcaat cccgattcgg ctatctgccg gcctgctacg ttaggatgga atcgcgcagg
    12241 ctcggcggaa atatcggata tatcgggttc aatttctttc ttgctccagc cgccgtgatg
    12301 aagaaattcg aggaggcggt caaatcgtgc agggagtgcc ctgggatcat catcgatttg
    12361 cgcggcaatc cgggcgggat cggagcgatg gccatcggta tggccgggtg gttcatcaac
    12421 cagcccaatc agcaactggg cgctatgtat acgcgtgacg ctacattgca tttcattgtg
    12481 aatcctcgcc tggagaccta tggcggcccg ctggcgatcc tggtggacga ctgctccgcc
    12541 tccacctcgg aggtgttcgc ccaggggatg agggaccttg gccgagcgcg catctttggc
    12601 atcaagacag ccggggccgc gctgccgtcg gtcttcgagc ggctgcccaa cggcgacggc
    12661 ttccaatacg cgatcgccaa ctacctttca aagggcggca aaactctgga gggcgccggg
    12721 gtggttcccg atgaagaggt aaagctcagc cgcgcagcgc tgctcgaggg acgcgatccc
    12781 gtcctcgagg cggcgctgcg gtggattcag gcgcaaagca cgcagaacgg ctccgggagg
    12841 tgagaatgat tgcgaagaaa gcttccaggt ttttgatagc ggcgctaacg atagaatggg
    12901 ctttggccgg cggaagttcg cgggcccagg agaaattgcc cgctgccgag accgtgcttg
    12961 accagtacgt ggaagtcacc ggcgggcgcg aagcgtataa gaaaatccat acgcagatca
    13021 cgatcgccac gatggagttt gtaggcaagg gaatcaaagc gaccctgacg tcctataagg
    13081 cgatccccca caacagctac aacgttgccg agatgcaggg aatcggcaaa atcgagcagg
    13141 gaactagcgg cgatattgcc tgggaacgct cgccgctgca agggccgcgg gtcaagagcg
    13201 gggaagagcg ggcatcggct ctccgcgatg ctgccttgaa tgcggaatgg cgcgagcttt
    13261 tcaagaaagc tgagatggaa ggcgtcgaga acgtcggctc gcgcccctgt tacaaaatcc
    13321 tgctcacgcc ggcggaaggg agccccgaga cacgctatta cgacaccaag acgcatctgc
    13381 ttgtcaaggt gaacaaagtg gtgaaaagcc agatggggga gataccggcg gaagtgacgc
    13441 tcagcgatta ccgggatgct ggcggcattc tggttcccca tcgcgtcttg cagaagggcc
    13501 tcggccagga gttcttgatt acgattgaaa aaatcgagca gaaccctgag attcctgcca
    13561 gccgcttcga tctccctgcc gacgtcaagg cgctgctcga aaaaagcggc gccggaggga
    13621 actgaaaggc catctgcgcg caaacgcgga acgccgccac gggaatatga acgaattctt
    13681 ccagcaaatc ggaacctttt tacaagcaaa tgtggccggc cgcgagctga atgacgaggt
    13741 ccggtccgag gctgcccgga ccgggccgca gccgccggcg ggccgtgcct attacctatc
    13801 ccttacgcgc gatccagtcg cggcggacat ccactacgtt gccgtagatc tcgatggttc
    13861 gtggctgctg cgttcgacgc ccgggggcgt cgacgcgctg ccgttttcgt cagccgattg
    13921 gagcgtgatc cgccggttcg cggaaaacat tgatccgtcc ttcctgccgc ggccgcaaaa
    13981 agaccagccg gccgtggctg tgggcaaccg gcatccggaa atttcactcc cggcggcatt
    14041 cgatgctttc ggccgcatcc tgcggtccaa gtgcgtcaat atggccagca ccgtgcagct
    14101 ttccgcaaca cgcgaaatga ccaccgccgc ccgcctcgcc ggccgtaccg gggaaaaccc
    14161 gacggaggtc gggcacacgc gcgtttccat cgagcacctc tatcacgccg gcctgtgggc
    14221 cgctattcgc gcgggatggc ggcatgggta caatgccgag gcagaccatt tcatcatcag
    14281 cggcgcgagc ggcgccgaaa ttgcgcattc catcgagcaa accaaggcgg ccattcgcgc
    14341 ggcggccgga tacacgaaat tcaccaccga tacctcgcgg ctgttccggc tcgaagccga
    14401 tccgcggcat cctcatgctt ggagtgacgc gcaggtcgag caggagttcg agaggattct
    14461 gaaccgggaa cagcagcgct gggtcttgag cgaattcggc cggagttttt ccatcgaggg
    14521 ccgcgtctat tcgttcacgc cggcggaaat ccgccggctg gcggtcaagt tcagccaaag
    14581 cttgcagttg aacgaagacc tttatgatta catccagcag gcgaagcgcg agagcggccg
    14641 ctcgacggac tttgatttcg agccgtcgat cgacgaggcg gaaaccttga ccacggcccg
    14701 cgagatgatc ttctatatgc actggctggc gtcgcgcggg cgccgggcgc aactggtgcc
    14761 gccgaatctc ggcttcaaga aacgccaggc gtaccccgag acggccgccg ctctccgcga
    14821 gtatgcagcg cacaagatgt ggcccgagct gctgccgcgc gtggagcggg aatttggcgg
    14881 ggaccctgtc aaggagttag gcgcacgggt gcgggagctg gccgacgtgg cgcgctattt
    14941 taacggcaca ttgagcatcc actcggggag cggcaagcag gctcgcgtgc tgcaaatgat
    15001 cggccgctcg accggcgggc gcgtgaacta caagatttcc ggcgagctcc aactgcaact
    15061 gctcgatgtg ctctcggaac agccggcagg ctcgccctgg cggtccctgt tcgaacgcat
    15121 ggtggatcgc tgtgttcaat ttgccgaggc cggagcgttc ggcgaggaga gccagttggc
    15181 gcggatctat aaagagaagg gactcggtta ttatctcggc gaccggctca agggccgcgt
    15241 ggatggcaat ctgtttctcg ttttctggct gggaaacctc gtcggctctc gcgacgcggc
    15301 cgatccgtcc ggcgatcatc gcttcttcaa ggaaaagctt gacgaattgc ccgccgcctt
    15361 ggttcaggaa gttcagcggc gcaacagcga ttacattgtc tggctcgcgg agcaattgcg
    15421 cccataacgg agcgccgcgc gcacaacatg cgcccggcat ttgcccgtcc ggcgcagcgc
    15481 ccttacggct cgagggaggc ggagagcgtg atgctcttcc ccgccgtgcg atcctcaatc
    15541 cgaatgacgc cttgttccag gctcacctcg tcaacgcgct ggaaaccctc cggcgttctg
    15601 gtatagaaga aaagaaagcg cgactcgatt ttggatttcg cggggagcca gcggtaaaca
    15661 ggcgcgtcga acatcgaacg caggtcaatc acctcgcggc gcggcacgtc aaagggctgc
    15721 gtgccgaatt cgaagccgcg ggccatcttg agcgtcggcg gatagttttc ccagttctgc
    15781 acccagggat actcctgcgg gtggaacagg taaccaacta acaaccgctt ttccggatga
    15841 agtgcggtca caaacgccca tttccgattc atgtccatca acgccgtcgt gtgatcgccc
    15901 gagttcgggt taggcggcgc ggcgcggagg tcgatcagtt ggccgctccc gtccggcgcc
    15961 atcggccagg tgaactccag agatgaagcc aggcgatgcg gcagagggcc gggcttcggg
    16021 tcgtgagggc gcgtcttggc gcgaaccgcc cgcagatcca cagcggtctt tcccggctcc
    16081 aggaacggcg agccgattgt ggcgtgctcg gcccagcaga tgggacggtc gaaggcgaga
    16141 aggctttcca acccgctttc cacatacacc acgttttccc catccaccag ccgcaccgtc
    16201 cgcgtgaagc gctcctcgac cagcggcaga tctacagaaa atgtgacgct ggccgttttg
    16261 ccgctcctgg cggccgccac gacccgccag gcctgtttgt tggcttcgcc gtggccgggc
    16321 agcccggccg cccgctcctg ctccgatacc ggtccgaagc catccacgca aacgaaatgc
    16381 ccggcctcga agccggaagg cggcctgcgc ccgtcctcac gcgccattcg cctcggattc
    16441 caatacgggc tcaacttttc ggggtcatcg cgcagcgcga ggttggcgaa ggccccaccc
    16501 tggctgagaa tggtcaattc cagcttgtca ttggagagga ccaacgccgg gatgccttca
    16561 aacgtggagc gcacgagaat ttccgcaggc ggagtctgcg ctaacgcgga caacgccaga
    16621 cctgcgaaaa gaagtgccag taagcgcata gtgcagacta ttataagaag cagagccatg
    16681 ccgccgcgaa aaataacaag ggcgttcggc ccggtgattg gctttctttt cctgctaagg
    16741 ccgtacgcat tggcgcagga ttcgcacccc tccgagcttc acgatccggc cgtgattcag
    16801 tatttccggc agggcggcca tcagcggctg gctcatttca ccacgctgct gcggcatccc
    16861 gtggaggcca gccaggattt gctgctggtt tggggcggcg tgcaggctcc gagctggacg
    16921 ggagctggcc tttggtggaa tcaaaccgat gtgatgggcc tattcttgca agagcgcgag
    16981 catcctcagc aggtgcacct gctggcgctg ttgctgaacg acgatcaaag ctgcgccgtg
    17041 gaggtggcta gaattgctcc gggtgagctg gtgctctgga ggacgggaga aaaaggggga
    17101 atattcgaca gcatcaagtt tttcttcgac gcccgttccc gccgcatctt ccggcgaatc
    17161 gagtacgctc ccttcacggt tcggcgcatt ctgatgaaag acggcgttct gcttttcctt
    17221 gccggcggcg gcaatcggct cctggtaatt cggctattgc gggaggctcc agccggccgg
    17281 attccattcc aggttttgtc ggaaaaggag gccgcgccgc tgttggccga ggcgccgcta
    17341 atcgtctctt cgacggcctc gggagaattt ggtgacttgc gaagggcgcc ggccagtctg
    17401 cgcttcggcc ccggaaagcg cttcgccttg gaaccggaaa caacgcagtc ttggggcacg
    17461 atctggcgca cagtgaccga gcgcgctggc ggccgcgccg cctccttcat gctgccgcaa
    17521 tcaagctgtg tagagtacgt ccaagcgcgg cgggaagcca tgcgccgcac ctttccggtg
    17581 attcccgcgg gcacgatctg ccagggcagc ggccctgaaa tcttggagac gatcggcgcg
    17641 tgggagatcg cgggcgacgg ccggctatgg cttgggaaga cattctatga cgccgagggc
    17701 agcgtagggg tgggaggctt cggctacttc gacccgggcg atcgcgagtt ccatatcttc
    17761 tcgccgccgg aagttcgaga ctggtcggtc tcggccttgc gggttggatc tgacgcggtc
    17821 tggctcgggc tgatgcatcg aggcgaatac ggcgattcgc cgggcgggtt gctgagatgg
    17881 gaccggagtt cgcacgcgat caatgcttac tccgtttctt ccatcgtcca tgacatcgcg
    17941 ctctccggga gcagaatcta tctggccgcc gcgagcggaa tctttgttct ggagaacggc
    18001 cggctgcaac agtatatcgt cgatgtcggg ccagacggcg gctatgaagt tgtgccccgt
    18061 tgacgctcag cctgtgtttc cgcggcgcac ggccttccga agcgccggct cgcgcaggga
    18121 gatgccagct aaccgggcag gggtatccgc cccgggcgcc atctaaaagc tgataaaatt
    18181 ttgagctgcc tgtgtcacgc catccccgcg gaaaatcaaa aaaacgtctg caccccaagg
    18241 ttgagaattt cgtggaagcc gctccctcgc cggcttccca tgcttcgctt cctgagcatc
    18301 gccgctcggc ggacgaaccc cgcgaaacca gcatattgcg aatcgagacg ctcatttggg
    18361 cgggggcctt gctggtcatc tacgcctgtg ttgcgacctg gggccagttc gatttcagcg
    18421 acctgatggg ttactacaac atgcaagcgg acgccttcct ggcggggcat ctctacatcg
    18481 cgcggaaccc gagcgaaggc tacttgatgg atatggtgcc gttcgagggg cggttttacc
    18541 tgcaatgggg gccgtttccc gcggtgctgc acatgatggc caagctcgcc ggcggaaaac
    18601 tcacggaccg ggtggcctgt attcttgcgg gatggctcac atcgctggtt tttttgaaga
    18661 tcgttgtcgt gctccggggg cgctacttcc ccgctttgcc aaagtggctc tgctgctggt
    18721 tcttttttac cttcgctttg gctaccccca cggcgattgt ggctctgcgc ggcacgattt
    18781 accacgaaag catcgcctgg gccgcgcttt tggtcctctt cgccttttgg gccctgctcg
    18841 agtatctcga tcggcaatcc gctccctggg tgttcctggc gggcgccggt gctggccttg
    18901 ccatcctgac ccgcgtgtcg ctcggactat atgcggccgg cctgctcgcc ggagtggccg
    18961 tcttgcagcg gcactggggc cggcgaaaga aggttgcggc gatgcacctg gcggctcttg
    19021 ccgcgccggc agccgccggc gtgctgctca tgatggctta caaccaagcc cggttccgct
    19081 ctccctggga gtatggcctg aaatatctgc ccgtggcggc cgccgacaag cctgcctatg
    19141 cgctgaaccg cgtgcccgaa aacttcctgc attatgtgct cgctcccatt cacgtgacgc
    19201 tagattgtcc ctggttcacc cacaagggct ggcagccgct ggtgaaaacg gaacgcgcgg
    19261 aagacatgtc gtccatgttt ctggcctcgc cgtttttgtt gctcggtttt ttggcctggc
    19321 ccttgtttcg cacgcgcgat gggcggattt tcccgctgaa agtgtttgcc gccgttgccg
    19381 gactttcggc cttcgccgtg tttttcagcc tcctgtgctt cgtcggaacc agccgccggt
    19441 atatgcagga ctttgtgccc atgctgatgg ttctggcgct gctcggagtg gcttatagcg
    19501 cgaaaaccgg aagcgattgg cggcgctggc aggccccagc ctggggcatt ttcgccctat
    19561 cggcgttact ccacgttcat ctggttttct tccagcctga gacctgggcg ccgctcgacc
    19621 cgaacgtcag caaggcgttt gtggctctcg gtccgctcgt gcggcgcgtg cttcccggac
    19681 accagctcga ttcgcggctg gcgattcatc acaatgatct gggacttctc tacatggacc
    19741 aggggcgtta cgcggaagcc atcccgcact tcgaacaggc caatcgttac atgcctgctt
    19801 cggtttcgat ccagcacaac ctgacttacg cgagacgact tcaggcagcc tccggcagat
    19861 aagtcagacg ggcgcattcg aacggctacg gttgcggcta gagtacgctc tcttcaggat
    19921 tccgagatgt tttatcagag cgagccggag tcctgcaacc tagattggca cgactctcgc
    19981 cggcctatcg ccggcaagtc attcctcctg gctgcgcttg gcagccggcg ggcggttcca
    20041 tcataatgta caatcacgca gtttttactg gctggtggat tccatcaaat tcatgggtgc
    20101 tcgaagaatg ttagggcgcc gggcggcgtg cctgctgatc tggacatttc tggagagcgc
    20161 ggcgctggcg gaatgcatcc gcgtatcggt acgcgaccct tccgggctgg cggccgccgg
    20221 ggccgccgtt cacgttgacg gcggaagcgc aaatctcacc gatgacgccg gcaatgccgt
    20281 gatctgcggc ctggccgcag ggcgtcacaa gatcacggtc gaaaaggccg agtttgcaac
    20341 cttgtccctg gttgcaaacg ctcccggcga acagacgatt acgctggctc tggagccgcg
    20401 ggtcgagtct ccggtcgtgg taaccggcac gcccgagccg gcgcaactgg ccgaagtgga
    20461 ccgcagcatc atcgtgatac ctgtcgaaaa tgcggtggtc ccggcgactt cgctggccca
    20521 attgctgaag acggacagca ccgtggatgt gcaagagcgc ggaattgacg gcacgcaggc
    20581 cgatgtgggg attcgaggcg gcagcttcag ccagacgctg gtgcttatca acggcatgcg
    20641 cgtgaacgat gcgcaatcgg gccaccacga tatggatctg cccttgcctt tggagagcgt
    20701 ttcacgcatg gaagtgctgc acggcagcgg ctcgacgctg tacggctcgg acgcggtcgg
    20761 cggagcggtg aacgtaatca cgcggcggcc cgaagcgacg gaactcaacg tgtcgtcggg
    20821 aatcggagac ttcggctgga atcgcgaagc gctctcgggc ggcttccggt tcgggaagtg
    20881 gagccagcaa gccgccgtgg cgcgcgattt ttcgaccggc tttcggcccg gccgcgattt
    20941 ccgcaacctg gctgtggcga gccaatcgtt tttcgacgag tcctttggtt cgaccagtct
    21001 gctgttcgct tataatgacc ggccttttgg agccaacgga ttttacggaa gcttcccctc
    21061 ctgggaagag accgggacca agcttgtggc gctgagccag acagtgggag aacaccgcgt
    21121 gcagttttcc tatcgccggc acaccgatca ctacgttctc taccgcgatc agccggaaat
    21181 cttccagaat ttccacacca gcggaacctg gtatgggtcc tacgctttcc accatgcctt
    21241 gtcgggccga gtggacctca gcgcgggtgc ggacggcctt tccgaggcta tcgaaagcac
    21301 gaacctgagc gatcaccggc gcgaaagggc ttcgggattc tttgtgctgc aagcgcggcc
    21361 tggtccgcgt ttgagcctga gcgctggctt gcgcgaagaa atctaccgga aatggagcgc
    21421 cgtcgccagc cctactctag cggccggagt gcggctcgga aaagggtgga aggcccgggc
    21481 cagcctcagc cgcaccttcc gcgtgccgac ctataccgaa ttgtattacc acgatccggc
    21541 aaatattgga aacccgaatc tgcggccgga aagcgcctgg agctacgaag ccggcctcga
    21601 ctggtggggc cgcggaggga cgaacatctc ggccacctgg tttgagcggc gcgagcgcaa
    21661 cacgatcgat ttcgtccggc ctgccggggc gtcggtctgg caggccatga acttcgagcg
    21721 gctcaacttt cacggcggcg agatcggcgc gcgcgaccgg atcgctgtcc gaggccggag
    21781 cgcagatgtt ggcatcagtt ataccatcat tcgggcgtcc cgcgagatca ttcccggcgt
    21841 ggcgtcgctc tacatcttca attttccccg caattcggtt gcttttaccg cggctgcgcc
    21901 ccttgggcgt tatgcggtgg tgcgcactcg cgtgggcgct ttcgatcgaa gctggcaacc
    21961 cgcggtcgcc ttgtgggata tttccgtggc cgggcaagga agccgcttcc agcccttcct
    22021 gcaaatcacc aatctggcga acgcctatta cgaatcgttc cccgggctgg cccagccggg
    22081 ccgctggatt cggggcggga tgaaaatcga gcttttgggc aagcgctgag ccgcgctctt
    22141 ttgcgcctgg cgggacgggc gatttttcca cagcgatctg attccgcgcc tgcggccgac
    22201 gcggtctggc gggatcttac ctgcaaagcc gcgggcgctg ctaaaatcgt ggcgtgcccg
    22261 catctgctga aaatgatttc cgggttctcc cgtcggttga tgaagttttg aagcaggaag
    22321 ccattgcgcg cctgaccgaa agatttccgc gccacatggt ggtggaagcc gtccgggagg
    22381 tgctgggaac attccgcgat aagctggtgg agggccgccg ggaagagatc gaatatcagt
    22441 tgccgcagct ccccgagaca gtgcggcagc gcctggatga cttgatctct ccctcgcttc
    22501 gctccgtaat caatgccacg ggcgtcgtcc tgcacaccaa cctgggccgc gctccgcttt
    22561 cggcctccgc ccttcgcgcc attgccggga cagccggagc atacaacaat ctggaatacg
    22621 atttgacgaa aggcgcgcgc ggccggcgcg acgtgcacgc cgcggcgctg ctcgagcgcc
    22681 tgctgggggc gcccgccatc gtggttaaca acaatgccgc ggcgattttt ttggctctgc
    22741 acgagctggc gtcgggcgga gaggtgatcg tttcccgtgg cgagctgatc gaaatcggcg
    22801 aaggcttccg cattcccgac atcctcgccc gcagcggcgc ccggctgcgc gaggtgggga
    22861 ccacgaaccg aacgcgcatc gaggactatc gcgcggcgat taccggcgaa acgcgcgcca
    22921 tcctgcgcgt tcatcacagc aatttccgga tcgtcggctt taccgatcgc ccttcgttaa
    22981 aagacctggt cgatgccggc agccaggctg ggattcctgt ggtggaagac ctgggcagcg
    23041 ggtgcctcgt ggacctgaaa ggctacggct tggaacatga gccgaccgta ggcgaaagca
    23101 tcgccgccgg aatagacgtg gttaccttca gcggagataa gctgctggga ggtccgcagg
    23161 cgggcatcct ggcgggcaaa aaagagtgga tcgcgcggat tcgccgcaat ccgctgtttc
    23221 gggcgctgcg ggttgacaag ctcatctatg cggcgctctc cgcgacgctg cgggactacc
    23281 tgctcgaaga tcgcgccggc atccccattt acgccatgct cgaccaaacg gctgatcaga
    23341 ttcgcgaacg cgccgaaaag ctcgttgcgg cgctccccgg catagaagcg gatctctgtc
    23401 ctggcagctc cgtcatcggc ggcggctcga ctccggctat ggaactcccg actacgctga
    23461 ttttgctgcg gcctccggaa gccgagtcgg cggcggcttg cgagcggctc ctgcggacgg
    23521 gcgccacccc ggtggtagcg cggctcgaaa agcagcgcat cattttggat ttacgaacgg
    23581 tcttaccgga acaggaggtt ctgctcgcag gcgctatccg aagcgcctgg agcaggaacg
    23641 gcttctaggg gccggacgac ctctgcctgc aacttcaatt cgccgctatg gggtgcagag
    23701 tgagccgctg cactctccag ttcgtgatct cgcccacgta cagttcgttc ggattgcggc
    23761 aatcaatctc atgaactgag ccgaattcct tcagcagctt gcccgccttg ccgaatttgc
    23821 cgaggattct gccatccagt tccattttgt aaatttcgcc gttttccatg tcctcggtgc
    23881 cgttggagtt agagctgaaa aggtactggt gagcgcccgg cgagatgcag accgcccagg
    23941 gagcgcccac attcagatac agcgttttga actccccgct gttatcgaag acctgaatgc
    24001 gccggtttcc gcgatcgccg acgtaaacat tgccctgcgc atcggtggcg atggtgtgtg
    24061 gggtgttgaa ctgcccgggc gccgttcctc tcgatcccca ggatttgagg aaacggccgt
    24121 ttttatcaaa cttggccacc cgcgagttgc cgtagccgtc tgagacgaaa atgttgccgg
    24181 cggcgtccca agcaacgtcg gtcggccggt tgaacacgtc ggtttgagcg cctgccccgg
    24241 cgggaagcgc gggggccgcg gcggccgccg gccgattggc tgccgcttgc ggcggcggaa
    24301 ccgtgggaac ggaaggcacg cgcagctcct ccgcctttct ccccatggtc atcaccactc
    24361 tcccctctgg gttgaatttg atgaccatgt tcgacccttc gtccacaacc cagatgttgt
    24421 cttgcgggtc gactcgcacc gcgtgcgcaa aaacaaatcc gtaaagcccg tgcccgattt
    24481 cgcggatgaa ttttccgttt cggtcgaatt cgaatagccg agatcccccg ttcgcaaacg
    24541 cccgcgaggt ccccatcgtc acgt
//
LOCUS       DASREK010000261         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1604731, whole genome shotgun sequence.
ACCESSION   DASREK010000261 DASREK010000000
VERSION     DASREK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604731"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /gene="rho"
                     /locus_tag="VFA54_14200"
     CDS             <1..457
                     /gene="rho"
                     /locus_tag="VFA54_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683792.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZS52014.1"
                     /translation="TRLARAYNTIVPPSGKVLSGGVDSNALQRPKRFFGAARNIEEGG
                     SLTIIATSLIDTGSRMDDVIFEEFKGTGNMEIHLDRKLVDKRVFPAIDVNKSGTRKEE
                     LLLTKEELNRVWVLRKVLSPLSSVETMELLLDKMGKTASNAQFLQSMSG"
     gene            487..1692
                     /locus_tag="VFA54_14205"
     CDS             487..1692
                     /locus_tag="VFA54_14205"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZS52015.1"
                     /translation="MGDASGRRRMSWRRHVLLPAASVLVGLIAAEFLLRLTPFGRPKL
                     DLTPFFQYDRTLGWKNRPFAKGTVYDWEYVTHLQYNSHGMRGPDLPYPKPPGTYRIIV
                     LGDSFIDAFMVPYGRRVTELLEKSLNQAGSQKHVQVVPMGVVGYSTDQELLWLESEGF
                     SYSPDLVVLMFFDNDIYGNGLPYFYGPKPQFLWDGQKLALHNVPVPEGLAGHHPHEQA
                     RWNLAGRFPKFHRWAAQHSRLYRLLIVDLQDLTPLPPYSPIYKKPETPEVRSAWDVTG
                     ALLARMQREVAGHGARFMVFYIPRREDVYRQEWAELETKLGLHPEDWDMRGVTDRFLS
                     ICREESLECIEPTAEFLEAARAAGQSGRRLYNKYDAHWNENGHRLAAEILAKKIRPDL
                     QSQTSRAKP"
     gene            2003..>3048
                     /locus_tag="VFA54_14210"
     CDS             2003..>3048
                     /locus_tag="VFA54_14210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS52016.1"
                     /translation="MRKPLNTATVVCVIAWCLLLSGRPAAAQLTTADVLGTVTDAAGA
                     VVTTAKVTIVNIATGDTRTVQTSSSGDFVVNLLPPGQYTVTVEAPGFRRSVTNLTLIA
                     GDRARADAQLQVGEVTQTVEVTAQAPALQTDSSTLRDTVAAQSVQDLPLNGRNYITLV
                     QTTVGATMGPSNSILSGTRPDERRQTANIVANGMNEVFNNQLIDGMDNNEREQFTILF
                     RPSIDSIEEVKVDTNSYPAEVGRAGGAVINLITKSGTNTFHGGLYEYFRNDKLNANDF
                     FANAGGIPRPEFRQNQFGGSLGGPIKKDRTFFFGDIEELRQIQSKNTGLLATATAYEL
                     ANPGDFTDQPGGIK"
BASE COUNT          634 a          896 c          908 g          610 t
ORIGIN      
        1 cacgcggctg gcgcgggctt acaacacgat tgtgccgcct tccggcaagg tgctctcggg
       61 cggggtggat tccaacgcgc tccagcggcc gaagcgcttc tttggcgccg cccgcaacat
      121 tgaagagggc ggctcgttga cgatcatcgc cacctcgctg atcgatacgg gcagccgcat
      181 ggatgacgtc atcttcgagg aattcaaagg caccggcaac atggaaattc accttgaccg
      241 gaagctggtg gataagcgcg tgtttccggc gatcgacgtc aacaagagcg ggacgcgcaa
      301 ggaagagctg ctgctgacca aagaagagct gaaccgggtt tgggtgctgc gcaaagtgct
      361 gagcccgctt tccagcgtcg aaaccatgga gctgctgttg gacaaaatgg gaaagactgc
      421 gtcgaacgcc caattcctgc aatccatgag cgggtaggcg gccgtgcgct aagatgacgc
      481 catgcattgg gtgacgcttc gggccggcgg cgcatgagct ggcggcgcca cgttctgctg
      541 ccggccgcct cggtcctggt agggctaatc gcagcagagt tcctcctccg gctcacgcct
      601 ttcggccggc cgaagttgga tctcacgcct ttcttccaat acgatcgcac ccttggctgg
      661 aaaaaccggc cgttcgctaa gggaactgtc tacgattggg aatacgttac ccatttacaa
      721 tacaattccc acggaatgcg gggtcccgat ctcccgtacc cgaagccgcc gggaacgtat
      781 cggatcattg tgctgggtga ttcctttatc gacgccttca tggttcctta cggccgccgc
      841 gttaccgagc ttctcgaaaa atccttgaac caagctggct cgcagaaaca cgtccaggta
      901 gttccgatgg gggtggtcgg ctacagcacc gatcaggagt tgctttggct cgaatcggag
      961 ggtttttcgt attcgcccga cctcgtagtt ctgatgtttt tcgacaacga catctatggc
     1021 aacggcttgc cctattttta tggtccgaag ccgcaattct tgtgggacgg ccagaagctt
     1081 gcgctgcaca acgtgcccgt gcccgagggt ctggccggcc atcatccgca cgagcaggct
     1141 cgctggaatc tggcagggcg gtttccgaaa tttcatcgtt gggcggcgca acactcccgg
     1201 ctgtaccgcc ttttgatcgt ggatttgcag gatttgacgc ctttaccgcc ttattctccg
     1261 atttacaaaa agcccgaaac gccggaggtt cgttcggcgt gggatgttac cggagctctg
     1321 ctggcccgaa tgcagcgcga agtagccggc catggcgccc gctttatggt tttttacatt
     1381 ccacggcggg aagacgtgta ccggcaggag tgggcggagc tcgaaaccaa gctcggcctg
     1441 catcctgagg actgggatat gcgcggcgtg acggatcgtt tcttgtcgat ctgccgcgag
     1501 gagtcgctcg agtgcatcga gcctaccgca gagtttttgg aagccgcgcg agccgccggg
     1561 caaagcggcc ggcggctgta caacaagtac gacgcgcatt ggaatgagaa tggccaccgg
     1621 ctggccgccg agatcctggc caagaaaatc cgaccggacc tgcaatcgca aacctcccga
     1681 gcgaagccct gaggaggcca ggctccgttg atagagctgg agaatgcagt cgcggagacc
     1741 gtcagcatcg actcctgtag cttctctttc cgcccccggt ctggcacgct cctcgctttc
     1801 atgcagccgg ctttcctcat ctcgcaagcg caaacgttta catggcaatc cgatcggacg
     1861 aaatcaggcg ttggcaatcc aagggtacaa acgctcttga ctgccgcgaa gcctagtctt
     1921 agaatggcgc gcagattatt tgggggttct gtgcaattag ctgctcctta caggcatttt
     1981 cttaagctta tggaggaaag ccatgaggaa accgctaaat accgcgaccg tggtgtgtgt
     2041 gatcgcctgg tgccttcttc ttagtggccg cccggccgcc gcccagctta caacggcgga
     2101 tgttctgggc actgtaaccg acgccgccgg cgccgttgtg acgaccgcca aagtaacgat
     2161 cgtcaatatc gcgaccggcg atacgcgcac tgttcaaacc agttcttcgg gagattttgt
     2221 ggtcaacctt ctgcctccgg gccaatacac ggtaaccgtg gaagcgcctg gtttccggag
     2281 gtccgtaacg aatttgacgc taatagccgg ggaccgggcc cgcgccgacg cgcagttgca
     2341 agtgggcgaa gtgacgcaaa ccgtggaggt aacggctcag gcgcccgcac tgcaaaccga
     2401 cagctccacg ctcagagaca ccgtggccgc gcagtctgta caggatttgc cgttgaatgg
     2461 gcgcaattac attacgctgg tccaaaccac cgtcggcgcg acaatgggtc cctcgaactc
     2521 gattctgagc ggtacgcgcc ccgacgaacg ccgccagacg gccaatatag tcgccaacgg
     2581 gatgaacgag gtgttcaaca accagctcat cgatggcatg gataacaacg agcgcgagca
     2641 gttcacgatt ctgttccggc cgtcgatcga ctccattgaa gaagtaaagg tggataccaa
     2701 cagctatccg gcggaggtcg gccgggccgg cggcgccgta atcaacctga tcaccaagtc
     2761 ggggacgaac acctttcacg gcggcctcta cgagtacttc cgcaacgaca agctgaacgc
     2821 caacgacttt tttgcgaacg ccggcggtat accgcgcccg gagttccggc agaaccagtt
     2881 tggcgggagc ctgggcggcc cgatcaagaa ggaccggacg ttcttcttcg gcgatatcga
     2941 agagctgcgc cagatccaga gcaagaacac cggcctgctg gcgacggcta cggcctacga
     3001 gttagccaat cccggcgatt tcaccgatca gcccggaggc atcaagat
//
LOCUS       DASREK010000262        17000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_457622, whole genome shotgun sequence.
ACCESSION   DASREK010000262 DASREK010000000
VERSION     DASREK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 17000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17000
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457622"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..845)
                     /locus_tag="VFA54_14215"
     CDS             complement(138..845)
                     /locus_tag="VFA54_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS52017.1"
                     /translation="MPASSIGKKFFYTSIAGEFDRMMNPYDLERRLEVVFEQLLLGSL
                     AGRLVLDAGCGTGWFAARAQYGGACVVALDLGPPLLEEARRKAPVFAAAGDALELPFR
                     DNTFDIVISSEAIEHTIDPARAIREMARVLRPEGTLVLTCPNRFWQWLVGFANCAGLR
                     RFDGYENFPSFHGLEQYIAAAGLALECHIGFHPWPFQLRPLWGVSRWVDAKLGGGWAR
                     RFMINQAVRASKRHVQP"
     gene            complement(851..2506)
                     /locus_tag="VFA54_14220"
     CDS             complement(851..2506)
                     /locus_tag="VFA54_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52018.1"
                     /translation="MKKKVFAALVVTGGVYFLYVPYALPVLDDWMFLQLFHQARAGGI
                     TGDLAFLRHLVDNTWIVQFRIFWASLLPVFALSFAADFAGKAYFLLAWTAHLLTAYLL
                     WRIAAAISGDSETAFAAGAIYMVFPAATNPLFWPVSNAVYYIQSLALLAWFWLTWKKL
                     VLQGDCRYSWKDFALLAGAIFTGEQILPALAILLPVALLLLGKRERRREYVRFCILHL
                     ALMAVLLGVYTFAVNAAPVWKGFQNRYASVHRWSLRPVAYLLFGALGLNRDFVPWPGH
                     WWADRALIGLFAAAALSFLGVVGPRQKPSNAGRFGEMLLWSVAGAILTYLPLARLSTF
                     EWRYLYVPSMFLVSGGAALLGVLRRPLRLALSFTALAYCITQTYLSMRHCWIPQSREA
                     RAALDAVAASRFAPYDIVILSGDHLANGLAPSFITGASWAQQSMLEHCTGAGHIQGAR
                     DLVVNEKGEMALYRRDSFRYFHRQEISRLRIFVRDPSTNRFLPKFVLALPVQDGRYQI
                     VRLGSSQQELPAPLSLEELKRLPQFGEIYFARRIHSHMKPTDL"
     gene            complement(2503..4551)
                     /locus_tag="VFA54_14225"
     CDS             complement(2503..4551)
                     /locus_tag="VFA54_14225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS52019.1"
                     /translation="MSRTAIAALCWAGLVLFTLYLLRVNDSYYRANTRTPTYDEAWYL
                     ETSLHLYHRLTRGGLVPFWEAYRGTFGTKAPLIAALPVPFYLVFGVSHYSALLVNSLF
                     IVITNFYLFLLGRRLFSPEVGLAAAVIYQTMPLAYGLSRAVMAEYGLTALVVVWIYYL
                     AASERLSRGPANFALGVTLGLGLLMKIMFPAFVAGPLLVAWFLRQRGAKPSVGGNFWL
                     WRLCARWPAAAIAVPAGIIAGPWYLSNWKTLLAFAWQSAYGQIGAEYAGGGFTQWLLA
                     FVNEGVGAYYTAALLVLGCIALLSGRRWMRGGYRELLLLAWLLPPFIAIAAGSNHLIR
                     FVAPLLPVFAIALAAAVFSLGRSWLLRAGLAVLLVIYPQRLFASLSYFSHGGGHARAI
                     SWGPFTLFSRDLGWAHPPAFEEQWRQQQIIEAIRQLDPGAVRPRYVVVGIEHVYLNAN
                     LLSYLNAYQEYPLLFTSFGYAESAPDRAVERIFAVDARFLVMGEGFQDLPPFLNRVNG
                     EIQARIHAGELPFRLCATVPLAHRMKALIYEKEVQWTVSPPGSNAAGPARALAADFTG
                     GVRFLGYDCRRRPGGLWDVAYYWSALSHIEQTYRLNAEYRRGGRVFLTEDYPLAAPRH
                     PPAEWQPGEVLRQSFTIYVKPGGAEDLEVDLSLTPWGIGPPQARAGSSEPFLRLRLTE
                     "
     gene            complement(4554..6644)
                     /locus_tag="VFA54_14230"
     CDS             complement(4554..6644)
                     /locus_tag="VFA54_14230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS52020.1"
                     /translation="MSGGASHSLAARTVICWALLAMFTGWLLLANYKYQVADRRPPTF
                     DDAWYLETSLYFYHRLTHGGFIDFLSAYAASFRTKAPLISVLPIPFYLLLGPRQHTAM
                     LVNFAFIAISSLYLFLLGRRLFSAAEGLAAAVFFQTMPLAYGLSRVFMADYGLTAMVI
                     AWIYYLVASEGLSRGRANFILGVLLGLGLLMKILFPIYVAGPLLAVLLLRRPHKQAGA
                     PKSGGEHWLWRACARWPLAAIAIPAAALASTWYAFHLFSILRYAWDAGYGGIGEQYGA
                     ADANHWVHYVINQGIGSYYAAVLLLFGGAALAACKARLEGNEAAGVLLGWIVPPMAAI
                     AAGSNREIRFVLPVLPAFALLLAGSVARLIPRPVMRLAAMLALAIFPLCLFATLSSPA
                     SSPEHYGAEHPVKAGPFILFAHELGWARPPDSRGDWNQDRILEAIEDMEPPGATPRYV
                     VLGVEHPYLNANLLRYLNAYKQYPLYFTSVGYAENSVERAIQRIYSLDARFLIMGEGF
                     HNFDLVEFLNRTNRDLQARLDHGELPFRLRAKVTLHGEIKAAIYEREEPWQRFAAGAA
                     EVPAHPLTVDFAGGARFLGYDWKRRNSQLCEISYYWTVLHRVTEDYRVNVEFRRGGNL
                     VLLQDYFIGQGRYPFYEWQPGEIVKQTTTVYLPEGAPPVEARVWLSAWGAGGPVQITN
                     PKAMIHQSMVPLRP"
     gene            complement(6641..8608)
                     /locus_tag="VFA54_14235"
     CDS             complement(6641..8608)
                     /locus_tag="VFA54_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52021.1"
                     /translation="MQKLTFQDGVAAAALWLAVLAVFAPGSGRLGFYYDDAVWMTSAP
                     PIMMRGLWAHMLGYIPGRNLFVLWEYILYRLWNPMNSLPAIHLVQTALDGLVVACFFL
                     VLRLLWLPAAPSLVAAGLFAFWPIHGETHFWPTLIPANLLTTLFVLLFAMTSLLLVRT
                     RSMSPGVCMLDAASFLAALFTYDQVFGVLVLLLVARLAARWACGGRDRIFFLLHVPYF
                     AAAIFYVRLKLRIRPGGAPAIRPDIWPVLRRNIADTISAVAGRIGLSQVAPLRAYITV
                     SDWLLGIAVAAGVVAMASWLLYRQSVQNRESAAALPFPRPWHVLLLGAVFCIAAYAPN
                     WLWWISPRHHYLPSVGLFMAVAVCLQWVFARASKTAWLCVLMAAGGLTAVFAACGRGE
                     SRLWEAAFAAKRQLFAELKPDLRGKDVLVLENFPLMLGDAYLIAPHDAAFGPELLYPE
                     PQWTNPRFIGSIGSSPAPQGIFLLTHALYGPQSFRYYSAGRELVVEYLSFDRNRFCYR
                     KNPPRTPPYEVLPAATGADRLDRAGAGSKSAAFTIQKASAKMEGGNLRVSLDFQSAQR
                     PGACLAATISFLHVGKFYQWGRWDGDNFNLIPILLVPEPKAGAPRALSWRGDIVLHHF
                     PQTARIRVGLFSAGRNADPVLLGQSEAAVEP"
     gene            complement(8626..10599)
                     /locus_tag="VFA54_14240"
     CDS             complement(8626..10599)
                     /locus_tag="VFA54_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52022.1"
                     /translation="MTLNPSFRRSDWVCAGLLWAATAAVFGTGASRLGFYYDDAGWLA
                     ELPSAGAHSLWALMTGYIPGRNLFPFWQFLIYSSAQSPASHLTEFHISQSTLDGLAVA
                     VFFCLLRMLSLPADVSIMAAGLFSFWPIHGETHYWLTAIPQNLLSTVFVLTFACTSVA
                     LARGVRLWWIWLLDAASFFCGLFTYDQCSIALGLIAGARAGAAMIRRNQSARVLAAMH
                     VAYAAALLFYAKLKLSTAAGAGPALSGQAWSSFPQNALATASDTAGKLWAHHMAELFQ
                     KASFADWLLAALAAATLTGLALWRLQEKADAPLWLSPARLLLLAGAFYIAAYIPVWVW
                     YISRRHHLLPSAGLFVGVAAAATVPAGSGRRLWRVLILLLFGGAILAFAAASRGESRY
                     WEESFAVKKELFRELQPDLAGRQALVLTGFPSYLGPAYLISPHDATFAPGLFFGKPSP
                     LDPGFTGSIGAVPAPGGVFIHTLLSIYGANQFLYEPAEKTLVVRYSGWDRGRLRFEKN
                     PPPRLFYEVVPDNSRGDADGGVQIAARREAGQTVVSFQVDARLPPHCYLAAVLSSFHD
                     GGFHRWGVFEKGFGYNVMPVLLSGSQEPPSGGSSGSGEPADARRASSAGRWRGLLRLH
                     DFPEAERLQLEFFKASKDHLPVPLGRAQTAVQP"
     gene            complement(10596..13745)
                     /locus_tag="VFA54_14245"
     CDS             complement(10596..13745)
                     /locus_tag="VFA54_14245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52023.1"
                     /translation="MSGPTPEQWRKIFLECDPGQDSGERFLPSRSCRNLTSAEHFARY
                     LFASGFCAGKRVLDVACGAGYGSYILKILGAAHVVGLDSDAVAVALARRNYQAAGLEF
                     RAGDATAWEAPDAPFDVIVSFETLEHVSDPEALLKNLRRQLAPQGLFLISCPHDARSP
                     WPSPFHLRHYTYHEFRDLVGRYFPEIYPFAQIHAVASLILPPEAAAADELAALPLPEA
                     YFDVAKSIESSDAFVLLCGPRTAAARGSAAVISKNLTEFLQEIYAGVNYVREHVPHLK
                     WQLAEAKAQIDRQARQLRARSRALEDQKKYTLELLEQLEAMRCSRTWRAMLACREAVV
                     SLARFSPGKLFALPFRLGRILTGPPMPPAPAGADTALTTAPILPQYLPHRRSEIRGWP
                     AERPLITVGIPCYNYGRFLREAIDSVLASTFQDFEIIVVNDGSTEPETLAVLEDLERN
                     PPAGARLRVVHQENQGLAAARNKGAALGLGKYVVSLDADDRIDPTYLEKVAWVLENQP
                     QYGFCYSLVQRFGAENTIWKTEPFSLEKALRYNHVPTGAAFRREAWVEAGGFRNELRG
                     QDDWNFWITLGAKGWDGYLIEEPLFFYRTHPTSMWSNIQISDREKTAEKIRALNSYLI
                     GQGDSRAAEEFQPPQDPVVRAAIDQPEPPPRPGVPLARRPHLKFGDGRPALLFAIPWM
                     QIGGAEQVVLQVMQGLAGNYALAAVTTLDVEHNWEAEFARLTPWIYHLAHLPLEDPQT
                     YLRELISLHGISGLVISSSALAYEAVPFLKQDGDLWTADIVHNTVAEGYFESSRRADR
                     YLDCHFAVGRPQRDALITRGGIRPEKIRLAPTAVDAALRFNPSTYAARLPEIRHSLGI
                     KGGETILTYTGRLAIEKDVPLFVRVVGELVRRHPQKKFQAFILGDGPERARVEHEIQL
                     QGLQGVIELLGFCDNVPEILAASHFALLTSRFEGSSITLLEAMSMRQIVLSTEAGSVR
                     DVITDGVNGFIISSRSPAEFAGRILEVLGNPFREAEIRERARQTIIEHYDLASMVRVY
                     AETIREALAERRDAAVRPA"
     gene            13900..15354
                     /locus_tag="VFA54_14250"
     CDS             13900..15354
                     /locus_tag="VFA54_14250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52024.1"
                     /translation="MPAPTVSSGQDSYVHQDLTGYYDGLAAEWASKKNQYYFQGLVRL
                     LKFLVPRGSRVLEIGCANGDLLAAVDPGYGVGIDLSSRMLEVARSRHPHLRFFQMDAH
                     RLTPEPELAEPFDYIILSDLVGDLYDVQGALQQLGKLCHPRTRIIITFYNFLWEPVLK
                     AAERAGLKTRQNLQNWLSPQDLANLLDLSGFEVIKQGRRMPFPLYVPLVSEILNSVLQ
                     ILPLLRRLGLQQYLVARPKPGGARPRYSCSVIVPCRNERGNIEPAVKRIPSMGEHTEV
                     IFVEGNSSDSTLEEIQSVMSRYQGPHELKLIEQGAGLGKGDAVRKGFAAATGDVLMIL
                     DADLTVAPEDLPRFFDAVESGAGEFVNGCRLVYPMEKEAMRYLNLLGNKFFGKMFTWI
                     LDQPIKDTLCGTKVLLREDYAKIAANRTYFGNFDPFGDFDLLFGAAKLHLKIVEIPVR
                     YRDRAYGSTNISRFRHGLLLLRMCVFAARKFKFF"
     gene            15354..>17000
                     /locus_tag="VFA54_14255"
     CDS             15354..>17000
                     /locus_tag="VFA54_14255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS52025.1"
                     /translation="MSGSAGWKRFPPWFYLIVAAAVAMILEWGVHEKGPTHWDDSWYL
                     AASVRLFDRFASEGVAGYWRGFEHALGDKAPLITVLPFPFFCLIGRSVFVIYLVNSAA
                     CLALAASLYLFTREFFGERVSLLAVFFALFSPLLLGLSRLFLVEYWLTALVVSSLLAL
                     ARWERTGCQRWAFSLGVLCGLGLLMKITFPLFVAPAVAVIFWRARRTPRSESSPAAAG
                     TLQAAVAAILVAAPALLLAGPWYLNNWDAITHRSFQESYFVPVHPTERQSPVGMAVDY
                     VFILLSHAVSAVQVVAAVAGLTVWAARRRNFPGAAVFYIAPWLITLPVFALSENRDVR
                     LIAPMAPAFAITSAVLFELVLNRWKRASRCLLAAAAVFSLLIAIGNSFPVFGSQTLRL
                     GRWLIFAPNMGYAFAPNPDRWPLDQVLERIAHRERLGPQSKMIVGLGADTWSFNSNNL
                     DLEAALLKYPFEFYTTAYTSSRNQVRRIMSSTQYFLWKDGGTQQPLGRFQGGPMTIDF
                     LRNGPLFREVPPGIPAPDGGLIRIFENASAGPDLFSSARPP"
BASE COUNT         3761 a         5120 c         5120 g         2999 t
ORIGIN      
        1 agtcgatttc accggcgagg cccctggcat gagcctggga aaaggcctgc aaataggtcc
       61 agtcgtcgag cacgggcagc gcatgcggca ggtagagcag gtaaaccgcc cccaggcagg
      121 cataaatagc aaaaacgcta cggctggaca tgacgtttcg acgcccgcac ggcttggttg
      181 atcatgaagc ggcgcgccca tccgccgccg agcttcgcgt caacccatcg tgaaacgccc
      241 cagagagggc gcaactggaa gggccacggg tggaatccga tgtggcactc gagcgcgagg
      301 ccggcggctg ctatgtattg ttcgagtccg tgaaaggaag ggaagttttc gtacccatca
      361 aagcggcgaa gtcccgcgca gttggcgaag cccaccagcc actgccaaaa cctgttaggg
      421 caagtaagaa ccagggttcc ttccggccgg agcacacggg ccatttccct tatcgctcgg
      481 gccgggtcga tcgtgtgctc gatggcctcg ctcgatatga cgatatcaaa cgtattgtcc
      541 cggaacggca gctcgagcgc atcgccggca gccgcgaaaa cgggcgcctt gcgccgcgct
      601 tcttccagga gcggagggcc gaggtcgagg gcgacaacac aggcgccgcc gtactgcgca
      661 cgggcggcaa accagcccgt gccgcaaccg gcgtcaagga ccagccggcc agcgaggctt
      721 cccaaaagca attgttcaaa caccacttcc agccggcgct ccagatcgta cggattcatc
      781 atccggtcga actcgccggc gatcgaggta tagaagaatt tctttccgat ggaagatgca
      841 ggcatgcgag ctacaggtct gtaggcttca tatggctatg aatgcggcgc gcaaaataga
      901 tttcgccgaa ttgcgggagc cgcttcaact cttccaggct cagcggcgcc ggcagctcct
      961 gctgggaaga ccccagacgg acaatttgat atcggccgtc ctggaccggc aaggccagca
     1021 cgaatttcgg taaaaaacgg ttcgtggagg gatcccgaac aaagatacgt agacggctga
     1081 tctcctggcg atggaagtag cggaacgaat cgcgccggta aagcgccatt tcccctttct
     1141 cattgacgac caggtcgcga gcgccctgga tatggccggc tccggtgcag tgctcgagca
     1201 tgctttgctg cgcccaactc gcgccggtga taaagcttgg cgcaagacca ttggcgaggt
     1261 gatcgccgga gagaatcacg atgtcatagg gagcaaaccg gcttgcggcg accgcgtcga
     1321 gggcggccct ggcttcccgg ctctgcggaa tccagcagtg gcgcatgctt aaatatgtct
     1381 gagtaatgca ataagcaagc gccgtaaaac tgagagccag gcgcagggga cgccgcagca
     1441 cgcccaggag agcggcgccg ccgcttacca gaaacataga gggcacatat aaatatcgcc
     1501 actcgaaggt gctcaaccgg gccaggggta aataggtgag aatagcgccg gccaccgacc
     1561 acagcaacat ctcgccgaac cggcccgcat tgctcggctt ctgacgcggt ccaaccaccc
     1621 ccaaaaacga taacgccgcc gccgcgaaca atccgattaa cgcccggtcg gcccaccagt
     1681 gtcccggcca gggaacgaaa tcgcgattca agccgagcgc cccaaaaagc agataggcca
     1741 ctgggcggag gctccagcga tggacgcttg cgtagcggtt ctggaagcct ttccaaaccg
     1801 gcgccgcgtt gactgcaaac gtgtagacgc cgagaagaac ggccatgagc gccagatgga
     1861 gaatgcagaa gcgaacatac tcccggcgcc tttcccgttt gccaagcagt agcaatgcca
     1921 cgggcagcaa gatcgccaga gccggcagaa tctgttctcc tgtaaaaatt gcgcccgcga
     1981 gcagggcaaa atccttccac gaatagcggc aatcgccttg aaggacgagc ttcttccaag
     2041 tcagccaaaa ccaggccagc agagccaggc tttgtatgta atagacggcg ttgctcaccg
     2101 gccagaacag cggattggtc gccgccggga agaccatata gatagcgccc gcggcaaatg
     2161 ccgtttctga atctccggat atcgccgctg caatcctcca aagcagatac gcggtgagca
     2221 gatgtgccgt ccaggccagc aggaagtaag cctttccggc gaaatcggcg gcgaacgaga
     2281 gcgcaaaaac cggcagcaag ctcgcccaga agattcggaa ctgcacgatc cacgtgttgt
     2341 ccaccaggtg gcgcagaaaa gccaagtctc ccgttatccc tccggcccgg gcttgatgaa
     2401 ataactgtag aaacatccaa tcatcgagca ccggcagcgc gtacggaacg tacaaaaaat
     2461 agactccacc ggtgacgacc agggccgcga acaccttttt cttcattccg taagcctcaa
     2521 tcgaaggaaa ggttcgctgg atcctgcgcg cgcttgcggg gggccgatgc cccaaggagt
     2581 gagagagaga tcgacctcca aatcttcagc cccgcccggt ttgacgtaaa tggtgaagct
     2641 ctgccgcaac acctctcccg gctgccattc cgcagggggg tggcgaggcg ccgccagcgg
     2701 gtagtcttca gtaagaaaaa ctctgccgcc gcgccgatac tccgcattca gacgatacgt
     2761 ttgctcgatg tggctgagcg cactccaata ataggcgacg tcccagaggc ctccaggacg
     2821 cctccggcag tcatagccga gaaaacggac gccacccgtg aaatcggccg ctagcgcgcg
     2881 ggcgggcccc gcagcgttag acccgggagg agaaaccgtc cattgcactt ccttttcgta
     2941 gatcaaagcc ttcatacgat gggcgagcgg cacggtggcg cacaatcgga acggcagttc
     3001 ccccgcatgg atccgagcct gaatttcgcc gtttacccgg tttaggaaag gcggaagatc
     3061 ctgaaaaccc tcgcccatga ccagaaagcg ggcatccaca gcaaagatgc gctcgacggc
     3121 ccggtcgggc gccgattccg cataaccgaa agatgtaaac agcagaggat attcctgata
     3181 ggcgttaaga taactcagca aattggcgtt caggtaaacg tgctcgatgc ccacgacgac
     3241 ataccgcggg cgcactgcgc cggggtccaa ttgccggatc gcttcgatga tctgctgctg
     3301 ccgccactgc tcttcaaagg caggcggatg cgcccatccg agatcgcgac taaagagcgt
     3361 aaacggaccc cagcttatcg cgcgcgcgtg gcccccgccg tgagaaaaat agctgagcga
     3421 tgcaaaaagg cgctggggat agatgacaag caggacggcc aagccggccc gaagaagcca
     3481 gctacggccc aagctgaata cggctgcggc cagagcaatt gcgaacactg gaagcagggg
     3541 cgccacgaaa cggatcaggt gattgctgcc ggccgcgatg gcgataaacg gaggcagcag
     3601 ccaggccagc agcagcaatt cgcggtagcc cccgcgcatc cagcgccggc cggataacag
     3661 ggcgatgcat ccaagcacca gcagcgccgc cgtataatag gcgcctaccc cttcattcac
     3721 aaacgcgagt aaccactgtg taaaaccccc gcccgcatac tccgcgccga tttggccgta
     3781 ggcgctttgc catgcaaagg ccagaagagt tttccagttt gagaggtacc acgggccggc
     3841 gatgatgcct gcgggaaccg cgatagccgc cgctggccat cgcgcgcaga gcctccatag
     3901 ccaaaaattt cccccaaccg acggcttggc tccgcgctgg cgcaagaacc aggccaccag
     3961 aagcggtccc gcaacaaaag ccggaaacat gatcttcatg agcagtccga ggcccagagt
     4021 cacgcccaag gcgaagttcg ccggtccccg gctaagccgc tcggaagccg cgagatagta
     4081 aatccaaaca acgacaaggg ccgtcagacc gtactctgcc atcacggctc gggaaaggcc
     4141 atacgccaaa ggcatggttt gataaatgac ggcagcagcc aggcccactt ccggcgaaaa
     4201 gagcctgcgg ccaagcagga ataaatagaa attagtaatt actataaaca agctgttcac
     4261 cagcaaggcc gagtagtggc tgactccgaa aaccaaataa aacggaacgg gcaacgcggc
     4321 gatcagcggg gctttcgttc cgaaggtccc ccgataggcc tcccaaaacg gaaccaggcc
     4381 gccccgcgtc agccgatggt agagatgcaa gctagtttcg aggtaccagg cttcatcata
     4441 agtaggggtc cgcgtattgg cccggtaata cgaatcgttc actcgcagca ggtagagcgt
     4501 aaataacaca agccccgccc agcacagggc ggctatggcg gttctgctca cggctagggc
     4561 ctcaggggaa ccatgctttg gtgaatcatc gccttcgggt tggtgatctg gaccggcccg
     4621 cccgctcccc acgccgataa ccagacgcgg gcctcgaccg gcggggcccc ttcaggcaag
     4681 taaacggtcg tagtctgttt gacgatctcg cccggctgcc actcatagaa aggatagcgg
     4741 ccttgcccga taaaataatc ctgtaacagc accagattac cgccccgccg gaactcgaca
     4801 ttgacgcggt aatcttcggt cacgcgatgc aagaccgtcc agtaataaga aatttcgcaa
     4861 agctgggagt tacggcgctt ccagtcgtat cccagaaagc gggctccgcc ggcaaaatcg
     4921 acggtcagcg gatgagccgg cacttccgcg gcgccggccg caaaccgctg ccacggctct
     4981 tcgcgttcgt aaatcgcggc cttgatctcg ccgtgcagcg ttactttggc tcgcaggcga
     5041 aacggcagct ctccatggtc gaggcgcgcc tggagatcgc gatttgtccg gttcagaaac
     5101 tccaccagat caaaattgtg aaagccctca cccatgatca ggaagcgggc atcgaggcta
     5161 taaatgcgct ggatcgccct ctccaccgag ttttccgcgt agcccaccga tgtgaaatag
     5221 agaggatact gtttataggc gtttaaatac cgcagcagat tggcattgag gtacgggtgc
     5281 tccactccga gcacgacata tcgcggagtc gcccccggcg gttccatgtc ctcgattgct
     5341 tcgagaatgc gatcctggtt ccagtcgccg cgggaatcgg gcggccgcgc ccagcccagt
     5401 tcgtgggcga agaggatgaa agggccggcc ttgacgggat gttcggcgcc gtaatgctcc
     5461 ggcgaagaag cgggggagct gagggttgca aacaagcaaa gcggaaatat cgccagcgcc
     5521 agcattgccg cgagccgcat caccggccgg ggaatcagac gggcgacaga gccggccagc
     5581 agcaaggcga aggctggaag gaccggcaga acaaagcgga tctcgcggtt gcttccggcg
     5641 gcgatagcgg ccatgggcgg cacaatccag ccaagcagca cgcccgcggc ttcgttccct
     5701 tccagcctgg ctttgcaagc tgcaagggct gcgccgccga acaagagcag gacggcggca
     5761 tagtaggagc cgatgccttg attgatcacg taatggaccc agtggttggc gtcggcagcg
     5821 ccgtattgct cgccgatgcc gccgtagccc gcgtcccagg cataccgcag aatgctgaag
     5881 aggtgaaagg cataccatgt ggacgccaga gccgcggcag gaatcgcgat cgcagccagc
     5941 ggccagcggg cgcaggcgcg ccaaagccaa tgctctcccc cggacttcgg cgcgccggcc
     6001 tgtttgtgcg gacggcgcaa aagaagcacc gccagcagcg gccccgcaac atagatcgga
     6061 aagagaattt tcatcagcaa acctaggccc agcaatactc ccagaatgaa gttggcgcgc
     6121 ccgcggctca gcccttccga cgcgacaaga tagtagatcc aggcgatgac catcgcggtg
     6181 aggccataat cggccatgaa gactcgtgaa agtccgtagg ccagcggcat cgtttgaaaa
     6241 aaaaccgcag ccgcaaggcc ttcagcggca gaaaacaacc ggcgtcccag gagaaataga
     6301 tacaagctcg agatggcgat aaatgcaaaa ttgaccagca tggccgtgtg ctggcgcggc
     6361 ccgaggagca aataaaatgg tatcggcagg acggaaatga gcggcgcttt ggtacgaaac
     6421 gaggccgcgt aggccgaaag gaagtcaatg aaaccaccgt gcgtgaggcg gtggtagaaa
     6481 tagagactgg tttcgagata ccacgcgtcg tcgaaagtag gcggccggcg atcagcgacc
     6541 tggtacttat aattcgcgag cagcagccag cccgtgaaca tcgccagcaa cgcccagcag
     6601 atcacggtgc gggcagccaa ggagtgggac gccccgccac tcatggttcg acagcagcct
     6661 ccgattgtcc gagaagcaca ggatcggcgt tccggccggc gctgaacaag ccgacgcgga
     6721 ttcgagccgt ctgaggaaaa tgatgcaaca cgatgtcgcc ccgccaggag agggcccgcg
     6781 gcgcgcccgc cttcggttcc ggcacaagca gaatgggaat caggttgaaa ttgtccccat
     6841 cccagcggcc ccactggtag aacttcccca cgtgcagaaa acttatagtc gcggcaaggc
     6901 acgcgcccgg gcgctgagcc gattggaaat cgagcgacac gcggagattg ccgccttcca
     6961 tcttagccga agctttctga atggtgaacg cggctgactt cgaacccgcg cccgcccgat
     7021 ccagccggtc cgcgccggtc gcagccggca gcacctcgta aggcggagtc cgaggcgggt
     7081 ttttccggta acagaatcgg ttgcggtcga aagaaagata ttcgacgaca agctcgcggc
     7141 cggccgagta gtagcgaaag ctctgcggcc cgtagagcgc gtgcgtcagc agaaaaatac
     7201 cttgtggggc cggcgagctg ccgatggagc cgatgaatcg gggatttgtc cactggggct
     7261 cgggataaag caattccggg ccgaatgccg catcgtgcgg cgcgatcagg taagcgtcgc
     7321 cgagcatgag cggaaaattt tccagcacca gaacgtcttt gccgcgcagg tcgggcttca
     7381 actcggcgaa gagctgtctt ttcgcggcga aagctgcttc ccaaagccgg ctctctcccc
     7441 gtccgcaggc ggcgaaaaca gccgttaggc cgcccgccgc catcagaaca cagagccaag
     7501 ccgtcttgct tgcgcgtgcg aacacccatt gcaagcacac ggccacggcc ataaacaggc
     7561 ccaccgaagg cagataatgg tggcggggcg aaatccacca gagccaattg ggtgcataag
     7621 ccgcaataca gaacaccgcc cccaaaagga gcacatgcca agggcgtgga aaaggaagag
     7681 cggcggcgct ctcacggttc tgtacgcttt gccgatagag aagccacgag gccatggcga
     7741 ccacgccggc cgcaaccgca atccccagca gccaatcgga aactgttatg taagcgcgca
     7801 agggggccac ttgcgaaagg ccgatgcggc cggcgacggc tgaaattgta tccgcgatgt
     7861 ttcggcgcag gaccggccag atatccggac gaatcgccgg cgcgccgccc ggcctgatac
     7921 gcagcttcag ccgcacgtaa aagattgccg ccgcgaagta ggggacgtgc agaagaaaaa
     7981 agatgcggtc gcgcccgcca caggcccagc gagcggcaag gcgagccacc agcagcagaa
     8041 ccagcacgcc gaaaacctga tcgtaagtga acagggcggc tagaaatgag gcggcgtcca
     8101 gcatgcacac gcccggtgac atgctccggg tgcgcaccag aagaagactg gtcatcgcaa
     8161 atagaagcac gaacagggtg gtcaaaagat tggcgggaat cagcgtcggc caaaaatgag
     8221 tttcaccgtg gatgggccaa aacgcgaaga gccccgccgc gaccagcgac ggcgcagcag
     8281 gcagccacaa cagccgcagc acgagaaaga agcatgccac caccagccca tccagcgcgg
     8341 tctggaccaa atgaattgcc ggcaggctat tcatcgggtt ccaaagccgg tagagaatat
     8401 attcccaaag cacgaaaaga ttgcggcctg gaatataccc cagcatatgt gcccacaggc
     8461 cccgcatcat gatcggtggc gccgaggtca tccataccgc gtcgtcgtaa taaaagccga
     8521 gacgccccga acccggagcg aacacagcca gcacagcgag ccacagcgcg gcagcagcca
     8581 ccccatcctg aaaggtcaac ttctgcattc tgattccgag ttccgtcatg gctgcacggc
     8641 cgtttgcgcg cgtcccaagg ggactggcag atgatccttc gaagccttga agaactccag
     8701 ttggagacgc tcggcttcgg gaaaatcatg gagccgcagg agcccccgcc accggcctgc
     8761 gctacttgcc cgccgcgcgt cagccggctc gccgctcccc gagcttccgc cgctcggagg
     8821 ctcctgggaa ccgctcagca gaaccggcat tacgttgtac ccaaaacctt tttcaaaaac
     8881 gccccaacga tgaaaaccac cgtcgtgaaa ggaactcaac acagctgcca aatagcagtg
     8941 aggcggcagc ctcgcatcca cctgaaatga cacgacggtc tggccggctt cacgccgggc
     9001 cgcgatttgg acgccgccat ctgcgtctcc acgggaattg tccggcacaa cttcataaaa
     9061 cagccgcggc gggggatttt tttcgaatcg cagccggcca cggtcccagc cgctgtatcg
     9121 gaccaccaat gttttctccg caggctcgta aagaaattgg tttgcgccat agatggaaag
     9181 cagagtgtgg atgaacactc cccccggcgc cggcacagcg ccaatgctgc ccgtgaagcc
     9241 cggatcgagc ggagagggct ttccgaaaaa cagccccgga gcaaaggtcg catcgtgcgg
     9301 agagatcaga taagcggggc ctaagtagga cgggaaacca gtcaacacca gggcctggcg
     9361 gccggccaga tccggctgga gctcccgaaa cagttctttt tttaccgcga aggactcctc
     9421 ccagtagcgg ctctcgcctc ggctggccgc cgcaaatgcc agaatcgcgc cgccgaacag
     9481 cagcagaatc agaacgcgcc aaagccggcg gccagatccc gcaggaaccg tcgccgcggc
     9541 ggccaccccg acaaaaaggc cggctgacgg gagcaaatgg tggcgccgtg aaatgtacca
     9601 aacccaaacc ggaatatagg cagcgatgta aaacgcgccc gccagcaaga gtagacgcgc
     9661 tgggctgagc cacaagggag cgtccgcctt ttcttgcagg cgccagagag ccagacccgt
     9721 cagcgtcgca gcggcgagcg cagccagcag ccagtcggcg aagctcgctt tttgaaacaa
     9781 ctcagccatg tggtgcgccc aaagttttcc cgcagtgtcg gacgccgtcg ccagggcatt
     9841 ttggggaaat gaggaccagg cttgcccact caatgccgga cctgcacccg cagcggtgct
     9901 cagctttagt tttgcgtaga acagcaaggc cgccgcatag gcaacatgca tggctgcgag
     9961 aacccgggcg ctttggttgc ggcggatcat tgccgctccg gcccgagcac ctgctatgag
    10021 acccagggcg atcgaacact ggtcgtaagt gaacaaacca cagaaaaagg atgcggcatc
    10081 cagcagccaa atccaccaaa gtctgacccc gcgggccagc gccacgctcg tgcacgcgaa
    10141 ggtcaggacg aagactgtgg agagaaggtt ttggggaatg gcggttaacc agtaatgtgt
    10201 ctcgccgtga atgggccaga agctgaagag gccggcggcc ataatgctca cgtcggcggg
    10261 aaggctcagc atgcgaagaa ggcaaaagaa aacggcgacg gcaagcccat ccagcgtgga
    10321 ctgagagatg tgaaactccg ttaaatggct ggcagggctc tgtgcagagc tataaatcag
    10381 gaattgccaa aacgggaaaa gattgcggcc cgggatgtag cccgtcataa gggcccaaag
    10441 cgaatgcgcg ccggcggaag gcagttccgc gagccatccc gcgtcgtcgt aataaaatcc
    10501 gagccgcgag gcgccggtcc cgaacaccgc cgcagtcgcc gcccacaaca ggcctgcaca
    10561 gacccaatcg gagcgcctga aagaaggatt tagcgtcatg caggccggac agccgcatcc
    10621 cggcgctcag caagcgcttc ccgaatggtc tcggcgtaaa cccgaaccat ggatgccaga
    10681 tcgtagtgtt caatgattgt ttggcgggcg cgctcgcgga tttccgcctc ccggaatgga
    10741 tttcccagca cttccagaat acggccggcg aattcggccg gagaacggga agagataata
    10801 aaaccgttta cgccgtcggt gatgacgtcc cgcacgcttc cggcttctgt gctcagcaca
    10861 atctgccgca tgctcatggc ttccagtaac gtaatggaac tgccctcaaa cctcgacgtg
    10921 agaagcgcga aatgggaggc cgcgaggatc tcgggaacat tgtcgcagaa gcccagcagc
    10981 tcgatgacgc cttgcaagcc ttgcagttgg atctcgtgct ccacccgggc gcgttcgggg
    11041 ccgtcgccga gaataaatgc ctgaaacttc ttttgcgggt gccggcggac cagttcgcca
    11101 accactctca cgaacagcgg aacgtctttc tcgatagcga ggcgcccggt gtaggtcaaa
    11161 atggtttccc cgcccttaat tcccagggag tgacggattt caggaagccg ggccgcgtaa
    11221 gtcgatgggt tgaagcgtaa ggcggcatcc acggcggtgg gcgccaggcg gattttctcc
    11281 ggacgtattc ctcctcgggt gatgagggcg tcgcgctgcg gccttcccac tgcgaaatgg
    11341 cagtcaagat agcggtccgc gcggcgggag ctttcaaagt agccttcggc aaccgtgttg
    11401 tggacaatgt cagccgtcca gaggtctcca tcctgcttca gaaaaggcac agcttcatat
    11461 gcgagcgcag aactggatat caccaggccc gaaatgccgt gaagagaaat gagttcccgc
    11521 aggtacgtct gcggatcctc tagaggcaga tgggcgagat ggtagatcca cggtgtaagt
    11581 cgggcaaact ctgcctccca attgtgctcg acatccaggg tggttacagc cgccagcgcg
    11641 taattgccgg cgagaccttg cattacttgc agcacgactt gttccgcgcc gccgatttgc
    11701 atccagggaa tggcaaacag cagggcgggc cggccgtctc cgaatttgag gtgcggccgg
    11761 cgcgccagtg gtacgcccgg cctgggcggc ggttcaggct ggtcgatcgc tgcgcggaca
    11821 accggatcct gtggcggctg gaactcctcg gcagcccggc tgtcgccctg gccgatcaag
    11881 tagctgttca gagctcgaat tttttccgct gtcttttctc tgtcggatat ctggatgttg
    11941 gaccacatgc tggtcgggtg ggtgcgatag aaaaacagcg gctcttcgat caagtagccg
    12001 tcccatcctt tggcccccag agtgatccag aagttccagt cgtcctggcc gcgaagctcg
    12061 ttgcggaacc cgccagcctc tacccatgct tcgcgccgga atgccgcgcc cgtggggacg
    12121 tggttgtagc gtaaagcctt ctccaggctg aacggctcgg ttttccaaat ggtgttctcc
    12181 gctccgaagc gctgcaccag cgaataacag aatccgtact ggggttgatt ttccagaacc
    12241 caggcgactt tttcgagata tgtcgggtca atccgatcgt ccgcatcaag cgagaccaca
    12301 tacttgccaa ggcccagcgc ggcgccctta ttgcgcgccg ccgccaatcc ctgattttcc
    12361 tggtgtacga cgcgaaggcg cgctccggcg ggcgggtttc tttccaaatc ctcgaggaca
    12421 gcgagagttt ccggctcagt cgagccgtca tttacgacaa tgatctcgaa atcctgaaag
    12481 gtcgaggcca gtacgctgtc aatcgcctcg cgcaggaagc gcccgtaatt gtaacaggga
    12541 attccgaccg tgatgagcgg gcgctccgcc ggccacccgc gaatctccga acgccggtgc
    12601 ggcagatact gcggcaaaat gggcgcggtg gttaaggccg tatcggcgcc cgccggcgcg
    12661 ggcggcatgg gcgggcccgt caggatgcgc cccaggcgaa agggcagggc aaaaagtttg
    12721 ccgggagaga acctggccag cgagaccaca gcttcccggc aagccagcat cgccctccag
    12781 gtgcgggagc agcgcatcgc ctctagttgc tccaaaagct cgagcgtgta tttcttctgg
    12841 tcctcgaggg cgcggctgcg ggcccgcaac tggcgggcct ggcggtcaat ctgcgccttc
    12901 gcctcggcca attgccattt gagatgcggt acatgttccc gaacataatt gacgccggcg
    12961 taaatctcct gcaaaaactc ggtgagattc ttgctaatta cggccgccga tccccgggcg
    13021 gctgcggtcc gtggcccgca caggaggacg aaagcatcgc ttgattcaat cgatttcgcc
    13081 acgtcgaaat aggcttccgg cagcggcagc gcggcaagct catcggcggc ggcggcttcg
    13141 ggcggtaaga tcagcgaagc tacggcgtga atctgagcga aggggtaaat ctcagggaaa
    13201 tagcgcccta ccagatcccg gaattcatgg tacgtgtagt gccggaggtg aaacgggctg
    13261 ggccaggggc tgcgcgcgtc atgcggacag gagatgagga acaaaccctg cggagcgagc
    13321 tgccgccgga gatttttcag aagggcttcc ggatcggaaa cgtgttccag cgtctcgaag
    13381 gagacaatta cgtcaaaggg cgcatccggg gcttcccagg cggttgcatc gccggcgcga
    13441 aactccagac ccgcagcctg gtagtttcgc cgggcgagag cgacggccac agcgtcggaa
    13501 tcgagcccca ccacgtgggc cgcgcccagt attttcaaga tgtaggaacc atagccggcg
    13561 ccgcaggcga catccaggac gcgcttgcct gcacagaagc cggaagcaaa caaatatcgc
    13621 gcgaaatgtt cggctgaggt gaggtttctg caagagcgcg acggcaggaa acgctcgccc
    13681 gaatcctgac cgggatcaca ttccaggaag atcttgcgcc actgttccgg cgtaggtccg
    13741 ctcaactcac tctcccgcgc cttggattct tcaaatcgca gaggctagaa acgccattct
    13801 accgccccgg cgactccccg tcaggcattt ccccggctag ggcgaaatgc atctgttatc
    13861 ataagcggga aactgctgta ttatcggctt ctttttgcga tgcctgcccc gaccgtaagc
    13921 tccggccaag attcctatgt gcatcaggat ctgaccggct actatgacgg cctggccgcc
    13981 gaatgggctt ctaaaaagaa tcagtattat tttcaggggc ttgtccggct cttgaaattt
    14041 ttggtgccgc gcgggtcacg cgttctcgaa atcggatgcg cgaacggcga cctcctggcg
    14101 gcggtcgatc cgggttatgg agtgggcatc gatctgagca gccgcatgct ggaggtagcc
    14161 cgcagccggc atccgcattt gcgttttttc cagatggatg cccaccggtt gacgcccgag
    14221 cccgaactgg cggagccgtt cgactatatc atcctgtcgg acctggtcgg cgacttgtac
    14281 gacgtccagg gcgcactgca acagcttggc aagctgtgtc acccgcggac gcgcatcatc
    14341 atcacgtttt acaacttcct ttgggagccg gtgctgaaag cggcggaacg agccggcctg
    14401 aagacccggc aaaatctgca aaactggctc agcccccagg atctggcgaa cctgcttgat
    14461 ctgagcgggt ttgaggtaat caaacaaggc aggcggatgc cctttccgct gtacgttccg
    14521 cttgtttccg aaattttaaa cagcgttctg caaattctgc cgctgctccg gcggctcgga
    14581 ctgcaacagt accttgtagc ccggcctaag cccggaggcg cgcgcccccg ctactcgtgc
    14641 tcggtcatcg tgccgtgccg caacgagcgc gggaatatcg agcccgccgt gaagcgcata
    14701 ccctctatgg gagagcatac cgaggtcatc ttcgtggaag gcaattcgag cgactcgacg
    14761 ttggaggaaa tccagtcggt gatgtcccgt taccaggggc cgcacgagtt gaagctgata
    14821 gagcaaggag caggcctggg caaaggggat gcggtccgta agggtttcgc ggccgctacc
    14881 ggggacgtgc tcatgattct ggacgccgat ttaaccgttg ctccggaaga cttgccgcgc
    14941 tttttcgacg ccgtcgagag cggcgccggg gaattcgtga acggctgccg gctggtgtac
    15001 cccatggaga aggaagccat gcggtatctg aacctgctgg gaaataaatt cttcggcaaa
    15061 atgttcacct ggattctcga tcaacctatc aaagatacac tttgcggcac caaggtcctg
    15121 cttcgcgagg attatgccaa aatagccgcc aatcgcacct atttcgggaa tttcgacccc
    15181 ttcggagatt tcgatttgct tttcggcgct gcgaagctgc acctgaaaat cgttgagatc
    15241 cctgtgcgct atcgcgaccg cgcctacggc tcaaccaaca tcagccgttt ccggcacggc
    15301 ctgctgctgt tgcgcatgtg cgtcttcgca gcccggaagt tcaagttttt ctgatgagcg
    15361 gatccgccgg ctggaaaaga ttccccccct ggttttacct gatcgtagct gccgctgtgg
    15421 cgatgatcct cgaatggggc gttcacgaaa agggccccac gcattgggat gacagctggt
    15481 atctcgcggc gtccgtccgt ttattcgacc gtttcgcttc cgagggggtg gccggctact
    15541 ggcgcggctt cgagcatgcg ttgggcgaca aagctcccct gattaccgtg cttcctttcc
    15601 ctttcttttg tttgattggc cggtcggttt tcgtgattta ccttgtgaac agcgcggcct
    15661 gcctggcgct ggcggcgtct ctttatctct tcacacgtga gttcttcggc gaaagagtgt
    15721 cgctgctggc tgtgttcttt gcgctctttt cgcccttgct cctcggcctt tcccgcctgt
    15781 ttctggtcga gtactggctc acggcgctgg tggtcagctc tctgctcgca ctggcaagat
    15841 gggaaagaac aggatgccag cgatgggcct ttagtctggg tgttttgtgc gggctgggac
    15901 tgttgatgaa aatcaccttc ccgctgttcg tggctcccgc cgtggctgta atcttctgga
    15961 gagcgcggag gacgccgcgc tcggaatctt ctcccgcagc ggcggggacg ctccaagcgg
    16021 ccgtggcggc catcctggtc gcggcgccgg ctcttttgct agcgggaccc tggtatctca
    16081 acaactggga cgccattacg catcgttcct ttcaggaaag ctattttgta ccggtgcatc
    16141 ccaccgaaag acaatctcct gtgggcatgg ctgtggatta cgtgtttatc ctgctgagcc
    16201 atgccgtttc cgccgttcag gtggttgccg ccgtggcggg gttaactgtc tgggcggcac
    16261 ggcggcggaa ttttcccggc gctgccgtct tttatattgc gccatggttg atcactctgc
    16321 cggtctttgc tctctccgaa aaccgcgatg tgcggctgat cgcgcccatg gcgccggctt
    16381 tcgccatcac cagcgctgtg ctgtttgagc ttgtgctcaa ccggtggaag cgcgccagcc
    16441 gctgcctgtt ggcggctgcg gcagtcttct cgctgctgat tgccataggc aattcctttc
    16501 cggtgttcgg ctcgcaaact ttgcggctag gccgctggct gattttcgcg ccgaacatgg
    16561 gctacgcatt tgcgcccaat cccgaccgct ggcctctgga tcaggttttg gagcgcattg
    16621 cgcaccggga gcggctgggc cctcaatcga aaatgatcgt ggggctaggc gccgacacct
    16681 ggtcctttaa cagcaacaat ctcgacctcg aagccgcgct cctgaaatat ccgttcgagt
    16741 tctacacgac tgcatatact tccagccgga accaggtgcg ccggatcatg agcagcacgc
    16801 aatactttct atggaaagac ggcggcacac aacagcctct gggccgcttc cagggcgggc
    16861 cgatgaccat cgactttctt cgcaatgggc cgctgtttcg cgaagtaccg ccgggcattc
    16921 ccgccccgga tggaggcctg atccggattt ttgaaaacgc cagcgccggc ccggacctgt
    16981 tttcatccgc gcgcccgcct
//
LOCUS       DASREK010000263         8273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_459095, whole genome shotgun sequence.
ACCESSION   DASREK010000263 DASREK010000000
VERSION     DASREK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459095"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFA54_14260"
     CDS             complement(<1..375)
                     /locus_tag="VFA54_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZS52026.1"
                     /translation="MSGRTPERERRRRASLWLPCVAALIAAAPAGRAQQAADFFRQNC
                     TSCHTIGGGRITGPDLKNVEQRKDRPWLVRFLQNPKAMIDSNDPYAVELQQQARGVIM
                     PTIPGMTPVVAQALLDLIEAESK"
     gene            complement(356..739)
                     /locus_tag="VFA54_14265"
     CDS             complement(356..739)
                     /locus_tag="VFA54_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52027.1"
                     /translation="MSCGRPSRRARCVGPHFKFEECYLYPSLERFLGEGYVKKLLNEH
                     DGIFRSVWKLAKLAEKETWSDADVKSAEANLDLIYEHPIGCDGLSLWMERLGAEERDE
                     LWKQMHATRRKGTGLFDYYYERQNA"
     gene            complement(859..1293)
                     /locus_tag="VFA54_14270"
     CDS             complement(859..1293)
                     /locus_tag="VFA54_14270"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZS52028.1"
                     /translation="MSRSSLYAIRALTYLACQAPGKLSGTRDIARHEGIPAAFLGKVL
                     QQLRRGRLLRAYRGTGGGYELALPPEKITLLAVLRCMDGASGLDSCVLEERDCSYNNP
                     CALHNSWGPVQARLLEFFESNTLAELVAARQSEARRSALHRE"
     gene            1374..2066
                     /locus_tag="VFA54_14275"
     CDS             1374..2066
                     /locus_tag="VFA54_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZS52029.1"
                     /translation="MSQFVRQLSSGMAVLESELFQLLEGTADAAFVVDAQGLTRSWNH
                     AAEKLLGYRASEVLGKPCADLFEGRGKMGAVVCRENCQVLECVRVGQEVPNFDLEVKT
                     RSGRNIWVNVSILRFQDRRTNRCYAVHLLRDIAQRRRTEDLAQKLLSAAKDLVVLAED
                     PTAQAPVSALTDQEQRVLRLLAAGKRPTEVARELQISPRTLRNHLHHANRKLGTTNRL
                     EAVTHAMRRGLL"
     gene            complement(2087..3223)
                     /locus_tag="VFA54_14280"
     CDS             complement(2087..3223)
                     /locus_tag="VFA54_14280"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS52030.1"
                     /translation="MRRTTFSVLVLLAAPLARAQTHPGDARRGAQLLEQQHCVLCHSI
                     RGAGGKSAPDLARRSLKAFSPSLLAETMWNHAPSMWQAMAARGIPIPSLEPPDVADLF
                     AYFYSLHYFTPPGDAARGKAVFISARCANCHAAAGGPGPPISQWKAAGDPIEWAQQMW
                     NHSSGMLREVEKRGIRWPELNAQQMVDLMTYAQNLPGAAARPPQLRFQDPGVGAKLFE
                     SKQCARCHTLGGPAEPGKIDLLRRAERFRTLLDFAAAMWTHGPKIHRRAREADIEIPA
                     FQDYEMAHLLAYLFDRHYFDERGNASRGARVYRSHRCPACHETVEAGAPQLSQFRGQF
                     TSLLLTSALWKHGPRMLAEMEKRGWKWPTFTGTEMADLIAYLNR"
     gene            complement(3233..5281)
                     /locus_tag="VFA54_14285"
     CDS             complement(3233..5281)
                     /locus_tag="VFA54_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52031.1"
                     /translation="MRRRVKNPENGPGLRPAVRLLVCIVSVVLPAVLGQAQPAGAQAP
                     AQTRQQEAPPAQAVAEQPPAPAVKEEQNRGSIDFGYRWILGQGGSHDVYRSIINLGGG
                     PKLFQAEASLRRPNGFLYDRIDLHASSWGGDPYNTALLDAARTGVYDLRIDYRNVKYF
                     SSIPSFANPFLGSGILFSERSFDIDRRYLDGQIDFRPGARLSPYLAYYHSSGFGRGVT
                     TFVSDSNEFPVGTHLRDGTDSYRGGVHVNLSKLNITLEQGGTLFKDDQQVGFSGVNPG
                     NRTTPVLGQMIVLKDLYQAYGARGSGIFSRGVIEARPSSKLSFSGQFLYSKPSIDVKY
                     LQQNSGNFLLLSTLQVYTGQLDQSLADANRPHTSGSWSSELRPLRRLRIIDSWFTDRF
                     HIAAGSLLGETFLTTTGLVTSNTTAAERLVMNYNQHQVEAVVDVASFLSLRGGHRYEW
                     GDASVPASRLTPQGERANLSRQIALAGVSVRIGSKIDVNADLEASAAGHTFFRTDLAD
                     YQRGRLRGRYHASKSVAFSGAFAVLNNQNAAQGARLDYQSRQSSLAVILTPREAKRWS
                     LLLDYTRGTLRSSLPYLVPQDRTQETSLYRDNGHFGGAALDLALWHGARLTIGGGFSL
                     DRGSRPTRYLQPRGSAVIPLWKLVSWTAEWRWFGFDETLFGFENFHTNLFATGLRIAK
                     "
     gene            complement(5278..6213)
                     /locus_tag="VFA54_14290"
     CDS             complement(5278..6213)
                     /locus_tag="VFA54_14290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HZS52032.1"
                     /translation="MIVFLLLLAGLWIPGRAQDPPSRTPVPTPPGETLAAEADAYAGS
                     ETCSLCHEDIARAFQQNAHFSVDNGRSKKIQAKAWASRACESCHGPGKAHAEAGDGKQ
                     IFVFTSAAGARANQQCLACHAGDATHSGRLMGSHNRNQLACTNCHSIHHPTARRLLAA
                     APNALCQSCHLEVGAEFQRPYRHKLQERAITCADCHNPHGGLPPHDQRRAAANEVICL
                     KCHGDKRGPFPFEHAPVRLTSCSTCHEPHGSANPRMLTRHDQSLLCLECHTTTPGFAG
                     ATPPAFHDLRSARFRNCTICHSKIHGSYISRDLLR"
     gene            complement(6210..>8273)
                     /locus_tag="VFA54_14295"
     CDS             complement(6210..>8273)
                     /locus_tag="VFA54_14295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HZS52033.1"
                     /translation="AAGRLQPGKHPVGRFRWMVGVAILGALAAALADQQPVFSSHEKA
                     FYLDAQQVNFVRPGLRIQIESASIDGQGVIQTQFKITDPKGLPLDRLGVTTPGVVNTS
                     FVAAVLPNGATQYMAYTTRIQTSPITNQSATQASSDTNGTYQQVGDGKYVYTFRTRAP
                     SGFDPSATHTIGVYASRDLSEFDLGTQFADATFNFVPNGSPVVNVREVVRTESCDRCH
                     DPLSAHGGARRSVSLCVLCHTPQTTDPDTGNTVNFPVMIHKIHMGSSLPSVQAGTPYQ
                     IIGFNQSVNDFSTVVFPADVRRCEMCHVPPAPITAAQQNNYLTKPSRAACGACHDNVN
                     FATGENHVNLPQVDDNLCSTCHIPQGELEFDASIKGAHVIPNHSSMLPGTTFEILKVD
                     NTAPGQNPTVTFRITDKAGNPIQPSDMTSLSLVNSLSSTVEYSGAVSESAKGATGSNG
                     VYTYTFNTRIPANATGTFAVGIEGYRTITLLPGTKKQMTARDAGLNKVFYYAVTDSKP
                     VPRRAVVALDNCNSCHSFLEAHGGIRNNVEHCVLCHNPNANDSARRPAGENPPESIHF
                     KSMIHKIHTGENLTTDFTIYGFGGSRNNFNDVRFPGDRRDCEKCHVNNSEQLPLPDGE
                     LPSQQPRGWFTPQQPIETACLGCHTEKQAAAHALLNTSPQLGEACEVCHGQDAEFSIN
                     KVHAR"
BASE COUNT         1535 a         2536 c         2585 g         1617 t
ORIGIN      
        1 ctttgattcc gcctcgatca gatcgagcag cgcctgcgcc accaccggcg tcataccggg
       61 aatggtgggc atgatcaccc cgcgcgcctg ctgttgcagc tccaccgcgt acggatcgtt
      121 gctgtcgatc atcgccttgg ggttctgaag gaagcgcacc agccaggggc ggtccttccg
      181 ctgctcgacg tttttcagat cgggcccggt gatccgtccg cctccgatgg tgtggcagct
      241 tgtgcagttc tgccggaaaa aatcggcggc ctgttgggcc cggcccgccg gcgcagccgc
      301 tatcagcgct gcaacacaag gcagccacaa cgacgcccgc ctccgccgct ctcgctcagg
      361 cgttctgccg ctcatagtag taatcaaaaa gacccgtgcc cttgcgccgc gtcgcgtgca
      421 tctgcttcca caattcgtcg cgttcctccg cccccagccg ttccatccac aggctcagcc
      481 cgtcgcagcc gatgggatgc tcgtaaatca gatccagatt ggcctccgcc gatttgacat
      541 ccgcgtctga ccacgtctct ttctcagcca gcttggccag cttccacaca ctgcgaaaga
      601 ttccatcgtg ctcgttcagc agcttcttga cgtaaccttc cccaaggaac cgctccagcg
      661 acgggtagag gtagcactcc tcgaacttga agtgcggccc tacgcacctc gcgcgccgcg
      721 atggcctgcc gcagctcaaa aagcgcgtct accacctgcc ggtgatccgc gtgaaagtcc
      781 tcgatcaatc ccataccggt ccatccttct atctgccgga cttgcggatg cacagatcgg
      841 caagtccgca attgcccttt attcccgatg caaagcgctt cgcctggcct ccgattgccg
      901 cgccgccacc agttccgcca gcgtgttgct ctcgaagaac tccagcaacc gcgcctgcac
      961 cgggccccag gagttgtgca aggcgcaggg gttgttgtat gagcagtcac gctcctccag
     1021 aacgcagcta tccagccccg acgccccgtc catacaccgc agcaccgcca acagcgtgat
     1081 cttctccggc ggcagcgcca gttcgtaccc ccctccagtc ccccgatagg cacgcagcag
     1141 acggccccgc cggagctgct gcaagacctt gcccagaaag gccgcgggaa tgccttcgtg
     1201 ccgcgcgatg tctcgcgtgc cggaaagctt ccccggcgcc tggcacgcca ggtaggttag
     1261 tgcgcgaatt gcatacaagc ttgatcgcga catcagcacg gcggctccgg cttcaggact
     1321 tccgcatcct ctaatccgta taagacttcc gcggccgtcc tacaatcgtc cgtttgtctc
     1381 aattcgtgag acaattgtcc tcgggaatgg ccgtgctcga aagcgagtta tttcagttgc
     1441 tggaagggac cgccgacgcg gcgtttgtag tcgatgcgca gggcctaacg cgttcctgga
     1501 atcacgccgc cgagaaacta ctgggctatc gcgcctcgga ggtcctgggc aagccttgcg
     1561 ccgacctgtt cgaagggcgc ggcaagatgg gcgccgtggt ttgccgcgag aattgtcagg
     1621 tgctggagtg cgttcgggtc ggccaggagg tgcccaactt cgacctggag gtaaaaacgc
     1681 gctccggccg gaacatttgg gtcaacgttt ccatcctccg cttccaggac cggcgcacca
     1741 accgctgcta tgcggttcac ttactgcgcg acatcgccca gcgccggcga actgaggatc
     1801 tcgcgcaaaa gctgttaagc gcggctaaag acctggtggt gctggcggaa gatcccacgg
     1861 cccaggcccc ggtttccgcg ctgactgacc aggagcagcg cgtactgcgc ttgcttgccg
     1921 ccggcaagcg cccgaccgaa gtggcgcgcg agctccagat cagcccgcgc accttgcgca
     1981 atcacctgca tcacgccaac cggaagttgg gaaccaccaa tcgcctcgag gcagtgacgc
     2041 acgccatgcg gcgcggcttg ctatgaacct gccggcttgc tcccagctac cggttcaagt
     2101 acgcgatcag atccgccatt tcggtgccgg tgaacgtcgg ccacttccag ccacgcttct
     2161 ccatttcggc gagcatccgc gggccgtgtt tccacaaagc gctggttaag agcaaagagg
     2221 tgaactggcc tcggaactgt gacagttgcg gcgctcccgc ctcgacggtc tcatggcagg
     2281 ccgggcagcg gtgggacctg tacacacggg cgcccctcga ggcattgccc cgttcgtcga
     2341 agtagtggcg atcgaacaga tacgccagca agtgcgccat ttcataatcc tgaaaggcag
     2401 gtatctcaat gtcggcctcg cgggcccggc gatgaatctt cgggccgtgg gtccacatcg
     2461 ccgcggcaaa atccagaagc gtacggaacc gctccgcgcg gcgcagcagg tcgatcttgc
     2521 caggctccgc cggtccccca agagtgtggc atcgggcgca ctgcttcgac tcgaacagct
     2581 tcgcgccaac gccgggatcc tgaaagcgca actgcggcgg cctggccgcc gcgcctggca
     2641 gattttgcgc gtaggtcatc aggtccacca tctgctgcgc gttcagctcc ggccagcgga
     2701 tcccgcgctt ttcaacctcg cggagcatgc cgcttgaatg attccacatc tgctgggccc
     2761 attcgatcgg gtcgccagcg gccttccatt gagatatggg cggaccggga ccgcccgccg
     2821 ccgcgtggca gttggcgcag cgcgccgaaa taaagaccgc cttgccgcgc gctgcgtcgc
     2881 ccggcggggt gaaatagtgc agcgagtaaa agtatgcgaa aaggtcggct acatccggcg
     2941 gctcgaggct gggaatgggg attccgcgag cggccatcgc ctgccacatg gagggcgcgt
     3001 gattccacat ggtttccgca agaagggatg gcgagaaagc cttgagagag cggcgagcca
     3061 ggtcgggcgc cgatttgcct ccagcgccgc ggatggaatg gcaaagcacg cagtgctgct
     3121 gttcaaggag ctgcgcccca cggcgagcgt cgcccggatg ggtctgcgcc cgggcgagcg
     3181 gcgccgccag gagaaccagc acgctgaacg tcgttcgtct cataaatatt cgttattttg
     3241 cgatgcgcaa accggtcgca aagaggtttg tgtgaaagtt ttcgaagccg aagagcgtct
     3301 catcaaagcc gaaccatcgc cactcggcgg tccagctcac cagcttccat aagggaatca
     3361 cggcgctgcc tcgcggctgg aggtagcgcg tcgggcggct gccccggtcc agagaaaagc
     3421 cgccgccgat cgtgagccgt gcgccgtgcc agagcgccag atccaaggcc gctccgccga
     3481 agtggccgtt gtcgcggtaa agcgaggtct cttgcgtgcg atcctggggc acaaggtacg
     3541 gtagcgagct gcgcaatgtc ccgcgcgtgt agtccagcaa aaggctccag cgcttggcct
     3601 ccctgggagt cagaatgacg gccaagctgc tctgccggct ctggtaatcc aggcgtgcgc
     3661 cctgagccgc attttgattg ttcagcacgg cgaaagcgcc gctgaaggcc accgacttcg
     3721 atgcgtgata gcggccgcgc agcctgcccc gctgataatc agccaggtct gtgcggaaga
     3781 aagtgtggcc ggcggctgac gcttcgagat ccgcattgac atcaattttc gagccaatgc
     3841 ggactgacac cccggccagc gcgatctggc ggctcaaatt tgcgcgttcg ccttgcggcg
     3901 tgagccgtga ggccggcacg cttgcgtcgc cccactcgta gcggtgcccg ccgcgcaagc
     3961 tgaggaacga ggcgacgtcc acaactgcct ccacttggtg ctgattgtag ttcatcacca
     4021 gccgctcggc cgccgttgta ttcgatgtca ccaggcccgt ggtggtgaga aacgtctcgc
     4081 cgagcagcga tccggccgcg atatggaacc ggtcggtaaa ccaggaatcg atgatccgca
     4141 gacggcgcag ggggcggagt tcactcgacc aggagccgga ggtgtgcggg cggttggcgt
     4201 ccgcgagtga ttggtcgagt tgccctgtat atacctgaag cgtgctcaac aatagaaaat
     4261 tgccgctgtt ctgctgcaaa tacttcacgt cgatcgacgg cttgctgtaa agaaactgtc
     4321 cgctaaagct cagcttcgaa gatggacgcg cctcgatcac cccccggctg aaaataccac
     4381 tgccccgcgc cccgtaagcc tgatacagat cctttagtac gatcatctgc cccagtaccg
     4441 gcgtggtcct gttgcctggg ttcacgccgc tgaagccgac ctgctgatcg tccttgaaga
     4501 gggttccgcc ctgctccaac gtgatattga gtttggataa gttgacgtgc acgccgccgc
     4561 gataggagtc ggttccatcc cgcaggtgcg ttcctacggg aaactcgttg ctgtcggaga
     4621 cgaaggttgt cacgccgcgc ccgaaaccgg aactgtgata gtaagcgaga tagggggaca
     4681 agcgggctcc gggccggaag tcgatctggc catccaggta tcgacgatca atatcaaaag
     4741 agcgctcgct gaacaggatt ccagacccca gaaagggatt ggcgaacgat ggaatcgaac
     4801 tgaaatactt gacgttacga tagtcgatgc ggagatcgta aacgcctgtc cgcgcggcgt
     4861 cgaggagcgc cgtgttgtag ggatcgccac cccacgaaga ggcatgcagg tcaatgcggt
     4921 cgtaaaggaa accgtttggg cggcgcaggc ttgcttcggc ctggaacagt ttcggtcccc
     4981 cgcccaggtt gatgatgctg cgataaacgt cgtgactccc tccttgacca aggatccacc
     5041 ggtatccgaa atcaatgctg ccgcgatttt gctcttcctt gaccgccggg gcaggaggct
     5101 gctcggcgac ggcctgggcc ggcggcgcct cttgctgtcg agtctgtgct ggagcctgcg
     5161 cgccggcggg ctgcgcctgg ccaaggacgg ccggcaaaac gaccgatacg atgcacacta
     5221 gcagccttac agcggggcgc aagccggggc cgttctccgg gtttttgacg cgccgtctca
     5281 tcgcagcaag tcgcggctga tataggagcc gtggatcttc gagtggcaga tggtgcagtt
     5341 gcgaaatcgt gcgctgcgca ggtcatggaa tgccggcggc gtcgcgcctg caaagcccgg
     5401 agtcgtcgtg tggcactcca ggcagagcag gctctggtca tgccgcgtga gcatgcgagg
     5461 gttcgccgag ccgtgcggct cgtgacacgt gctgcaagaa gtcagccgca ccggcgcgtg
     5521 ctcgaaggga aacggaccgc gcttgtcgcc atggcatttc aaacagatga cctcgttggc
     5581 ggcggcccgc cgctgatcgt ggggcggcag gccgccgtgg ggattatgac aatccgcgca
     5641 cgttatcgct ctttcctgca atttgtgccg gtacggacgc tgaaactccg cccctacctc
     5701 cagatggcag ctctggcaaa gggcgttggg ggctgcagcc agcagcctgc gcgcggtcgg
     5761 atgatggata ctgtggcagt tcgtgcaggc gagctggttg cgattgtgcg atcccatcag
     5821 gcgtccgcta tgtgtggcat cgcctgcatg gcaagcgagg cattgctggt tcgcgcgggc
     5881 gcccgctgca cttgtgaaga cgaaaatctg tttgccatcg cccgcctcgg cgtgagcctt
     5941 gcctggcccg tggcagcttt cgcaggctcg gcttgcccat gccttagctt ggattttctt
     6001 gctgcggccg ttatccaccg aaaaatgagc gttctgctgg aaggctcggg cgatgtcttc
     6061 atggcaaaga ctgcaggttt ccgagccggc ataggcatcc gcctcagccg ccaaggtttc
     6121 tccgggcggc gtgggaaccg gggtccggct cggagggtcc tgcgcccgcc cagggatcca
     6181 aaggccggcc agaagcagca ggaaaacaat caccgggcgt gaaccttatt aattgagaac
     6241 tccgcgtcct ggccgtggca cacttcgcac gcctccccca actgcggcga agtattgagc
     6301 agcgcgtgag cagccgcttg cttttctgtg tggcagccga ggcacgccgt ctctatgggc
     6361 tgctgcggag tgaaccagcc gcgcggctgc tgcgaaggca attcgccgtc gggcagcggc
     6421 aactgctcgg agttgttcac gtggcatttt tcgcagtcgc gcctgtctcc cggaaaacgg
     6481 acgtcattga aattgttccg gctgcccccg aaaccgtaga tggtgaaatc cgtcgtcaga
     6541 ttttcgcccg tgtgaatttt gtgaatcatg gacttgaaat ggatgctctc aggcggattc
     6601 tccccagccg gccgccgcgc ggaatcattg gcgttcggat tgtgacagag cacgcagtgc
     6661 tcgacgttgt tgcggatgcc tccgtgcgcc tcgagaaagc tgtggcagga gttgcagttg
     6721 tcaagggcca cgaccgcacg gcgcggaacc ggcttggaat cggtgaccgc gtagtaaaaa
     6781 accttgttga ggcctgcatc tcgcgcggtc atctgcttct ttgtgccggg cagcagagtg
     6841 atggtgcggt agccttcgat gcccacggcg aaagtccccg tagcattggc gggaatgcga
     6901 gtattgaagg tgtatgtgta aacaccgttc gatccggtag cgccttttgc gctctcggag
     6961 accgcgccgg aatactcgac ggtggaactc agagagttca ccagcgacag ggaggtcata
     7021 tcggagggtt gaatggggtt tccggcctta tccgtgatcc ggaaagtgac ggtggggttt
     7081 tgccccgggg ccgtattgtc caccttgagt atctcgaagg tagttcccgg cagcatggag
     7141 gaatggttgg gaatgacgtg ggcgcccttg atcgaagcgt caaactccag ttcgccttgc
     7201 gggatatggc aggtcgagca taagttgtcg tctacctgcg gcaggtttac atggttctcg
     7261 ccggtggcga aattcacgtt atcgtggcag gccccacaag ccgcccgtga gggcttggtc
     7321 aagtagttgt tctgctgggc ggcggtaatt ggggcgggcg ggacgtgaca catttcgcat
     7381 cgccggacat cggctggaaa caccaccgtt gaaaagtcgt tcacggattg gttgaagccg
     7441 atgatctggt agggtgttcc ggcctgaacg ctgggcaggc tcgatcccat gtggatctta
     7501 tggatcatga cggggaaatt taccgtgttg cccgtgtccg ggtctgtggt ttgaggcgtg
     7561 tggcatagca cgcacaagga aacactgcgg cgcgctccgc cgtgagccga caagggatca
     7621 tggcagcgat cgcagctttc cgttcgcacc acctcgcgca cgttcaccac gggcgagcca
     7681 ttggggacaa agttaaaggt ggcgtcggcg aattgcgtac ccagatcgaa ttcggagagg
     7741 tcccgcgatg cgtagacccc gatggtgtgg gtggcggaag gatcgaaccc ggagggcgct
     7801 ctggtcctga atgtgtaaac atatttgccg tcgccgactt gttgatatgt cccattggtg
     7861 tcgctggatg cttgcgtggc gctctggttt gtgatcggac tggtctggat acgcgtggtg
     7921 taggccatat actgcgtcgc cccgttcggc agcacggccg ccacaaagct ggtgttcacc
     7981 acgccgggcg tagttacgcc caatcggtct agcggcagcc cttttggatc cgtgatctta
     8041 aactgagtct gtatgacgcc ctgcccgtca atcgacgccg actcgatctg gattcgcaaa
     8101 ccgggacgaa caaaattcac ctgctgcgcg tccaggtaaa acgccttctc gtgcgacgag
     8161 aagaccggct gctggtccgc gagcgcagcg gccagcgcgc ccagaatcgc cacacccacc
     8221 atccagcgaa acctgccaac cgggtgcttt cccggctgaa gtcttcctgc cgc
//
LOCUS       DASREK010000264         8036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_459402, whole genome shotgun sequence.
ACCESSION   DASREK010000264 DASREK010000000
VERSION     DASREK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459402"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3109
                     /locus_tag="VFA54_14300"
     CDS             <1..3109
                     /locus_tag="VFA54_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZS52034.1"
                     /translation="WTDPLVVFSRNDGNLTRMEVLLALGEINQVLKVVANGIEIPAGV
                     SGQDMTGSGWYNVISAGTRLGGFDPDFADSSGNPAGDPYGSMACLSVVAPNQINNGTS
                     LPRLQVLLEGAKIETFNANGNSLGYSFNNNPVWVLLDVLRHSNWKLQELDLASFAQAA
                     AFCDETIAAIDNNGNPISIPRFQCNLLIRTRRAAADVIRGIRNNARLMLSYTTDGKLS
                     VSVENSLALQQPQKAYGSNAPEMVNGGWPVYVYSDGSGGSPRSGILRDGNEASTVKLW
                     SRATADTPNRFAIEFQDMFNEYQQDSFDIVDVDDVARTGQEITGRAVVDGIPNYDQAA
                     RALKFLLDKSIKGNRYIEFDTTVKALGQRVGDIVTVTYAKEGFLNQPFRILKIAPGMN
                     YRTAHITAQIHDDAWYEDTNGQLTAIPAGRRQPAAGTNLPNPVPGTEVDSNGLVQFTI
                     TEVEVTGTDGTILAEVDVQFSPPSMGQSRLVGIPLVSLQPTVLSAGGSLAGGQTLYYA
                     VTATDSNGEETSPSFTIRAIIPPGSSTNSVQLNGLSFTAGTASFNVYRGSFPTQLYRI
                     ASGASPAPAFTDTGFPVQLTSSPDPFYDHANFYWRMEETEEKFATIIALNQVGDAGLN
                     LTPNAYIGHVVRLVSGQGAGQERTVVANTATTITVDRNWDAMPDASTHFVVSEAAWHF
                     GARARSSPARFQIPNLKGSVVQITGRSANVNNIESPERLAVVSRWKIGGGGTGVADVG
                     PPPAPSFGTSVHGDGTIRFLGIAFGELANTQGITSGTFLLHYRDELEGISAIQLNAGI
                     DASATTVTLNAPGGATAGDLIQLGSEIMQVLEVQNAGLAYSVSRAQQGTPAGPHDANT
                     AVYRLQSRTIVVAFEKNFFSTPEAGSWSHSEWAPDIRLASVEFFVTNAFGASPTAVNT
                     YTQLAGNGQRTLRGGQLDLQVEGVLGIQSDAVPEITVGQAASVSSVSARVELAPAGAD
                     ITLVVKVDGAAWATLTIPDGQLQANGIDGAELPPLMTGSMVSMDITGVGTTFPGQRLA
                     ASIRL"
     gene            3139..6393
                     /locus_tag="VFA54_14305"
     CDS             3139..6393
                     /locus_tag="VFA54_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52035.1"
                     /translation="MPEKIYKLQPHRTMHLRGFDRRGAAAALWGASDAGFTMSGVFRD
                     PADFCVLILFDADDVFSHPRWRYLPDFDFTGITLQFDVTYTNLQPLDSPKFPSIDWDK
                     LDIIKADGATAQVKLWDHAAKQSGGFSAASLTYTLHGVPAAFDRVTLWYQNLAFDYIV
                     PGATSVDYQFFAAGTGTVHSITIGTNTYSYTETAGDSSPAVASALAAAINGVDPNAAA
                     SASANHVILTPTLDNGSSVACSATDGNSLATLYQVQASTVLRSIRDQINGANWPAQGA
                     PLGLRAAVSGSSLVVTAARYGTVNTSGTTVTWASGEKFTGIDPAGTIQIGGAVYNISS
                     VDSPTQITLAASAGAQSGAAYLADRGGEDGNMIALCELHKNDNLYFTPQGAAQMTGGD
                     SAATWRVTLDFSSLGITSIRQMWMTFAPQLTNAGAYSPQEWTAVFSNWTVSDANLKRE
                     LKVAGPGSVRVGSQDSWANYTGSGWSSESGFYWLGFAKRTAQAGDSVAVEYHCQHTHD
                     LYIGTSLYKDRGIFAAVLDGGGATDLDCYLDAEPAVVTRRLVRSGVPAGKHTLTLILK
                     DAKNADSSGFNAYFDYLEAAVASDVPDAPQVYGDRSVATDFDTDHAYKLSPQRLVWMI
                     GKSGLHGDIDHYAGVFWWNQRKRVGGAFPSLTVTFSGTWADGDAAFLQIGGNAFNDGA
                     GTVMGKSVFPADTTETIAAHFASFINETFVGVWASSAASVLTITCRSPEYSFTFFTNK
                     SSAAGTISVSGSLSGGVEGTWQIDDTITPVINRAAADWHRDYFAEIAAKGWTATAAFS
                     MELVDPPDNPPTAAWAQRFRDNAAVQTDTGFASLQSTQCAFSGAVAAYQKQAFQEMAG
                     LMKLAGLTPWLQFGEFLWWFFTNYDAGSNPGGGMAYYDSYTAGQAQAVLGRPLASFLT
                     PGDDPSQNNYTDANFLRSQIKTHIDAIRSYVLSTHPDAKFELLWPLDVNYPPAKRLNR
                     YVNLPAEYQQPVGSGLDRIKMEGLSFGATERNIDHARQAMRFPYTPPLAWAKSDVRYL
                     LPWFNGGCPWAREYLAGCRELLPNISFWAWDHLNLFGWPLPLPAETRRSALVNT"
     gene            6558..7109
                     /locus_tag="VFA54_14310"
     CDS             6558..7109
                     /locus_tag="VFA54_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52036.1"
                     /translation="MQSIFDNCGRRIAKATCGDATRNALLAAIAANESGGRPRHYRFS
                     FENYGCLLGLVKGDASGLNGLTRRQLEKRLREPGPETESGALLKKLAGLYGYTQIPGY
                     YSIVWNASLEALMEEESHFSFAAALLDRICRQFHLDPETQAAAIGRCWNAGHASGRAS
                     SALYSWRLVERMKLYRGIEESGT"
     gene            complement(7247..>8036)
                     /locus_tag="VFA54_14315"
     CDS             complement(7247..>8036)
                     /locus_tag="VFA54_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52037.1"
                     /translation="CLRELGRPVAEIAEAGEAPLAGGELEAAEQECSSLRAKIEALGP
                     VNVLALEEYQEAQQRLEFLDTQRKDLFESIRDTQQAIGEIDSVSRKQFLDAFERVNEN
                     FRKTFSTLFGGGVGEMRLTDETNIAESGIDIVASPPGKRLQNVLLLSGGEKALTAIAL
                     LMAVFQYQPSPFCILDEVDAPLDEANIVRFTRLIRQMSQQTQFILITHSKTTMEVAQA
                     LYGVTMQEPGVSRLVSVRFGEKSDHAVRPLVPPPARGMGNVARV"
BASE COUNT         1616 a         2434 c         2504 g         1482 t
ORIGIN      
        1 atggaccgat ccgctggtcg tcttctcccg caatgacggc aatcttacgc gcatggaggt
       61 gttgctggcg ctgggggaga tcaatcaggt gttgaaggtg gtggccaacg gcatcgagat
      121 tcccgccggc gtttccggcc aggatatgac ggggagcggc tggtacaacg tcatttccgc
      181 aggaacgcgg ctgggagggt ttgatccgga tttcgcggac agcagcggca acccggccgg
      241 cgatccctac gggagcatgg cctgcctctc ggtggtcgcg ccgaatcaga tcaacaacgg
      301 aaccagtctg ccccggcttc aggttttgct agaaggcgcg aagatcgaaa ccttcaatgc
      361 aaatgggaac tcgctcggat attcgttcaa caacaacccg gtttgggtat tgctggatgt
      421 gctgcggcac agcaactgga aattgcaaga gcttgacttg gcgagcttcg cccaagctgc
      481 ggcattttgt gacgaaacca tcgcggcgat cgacaacaat ggcaatccga tttctattcc
      541 cagattccag tgcaatctgc tgatccgcac gcggcgggcc gccgccgatg tgattcgcgg
      601 cattcgcaat aacgcccgcc tgatgctgag ctacacgacc gatggaaagc tcagcgtgag
      661 cgtggagaat agcctggcgc tgcaacagcc gcaaaaggcc tacggatcca acgcgccgga
      721 aatggtaaat ggaggctggc ccgtttacgt ttacagcgac ggctccggcg gcagtcctcg
      781 ctcgggcatt ttgcgggacg gcaacgaggc atccactgtg aaactctggt cgcgagcgac
      841 agcagacacg cccaatcggt tcgcgatcga gtttcaagat atgtttaacg aataccagca
      901 agacagcttt gacattgtgg atgtagacga cgtcgcgcga acgggccagg agatcacagg
      961 ccgcgcagtg gtggatggca tcccgaacta cgatcaagcc gcacgggcgc tcaagttcct
     1021 gcttgataaa tcgataaaag gaaaccggta tatcgagttc gatacaaccg tgaaggcgct
     1081 ggggcagcgc gtcggcgata tcgtcaccgt tacatacgcc aaagagggat ttctcaacca
     1141 gcccttccgg attctgaaaa tcgcgccggg aatgaattac cggacggcgc acattacagc
     1201 gcagattcat gatgacgcct ggtatgaaga taccaacggg cagcttacgg cgatccccgc
     1261 gggcaggagg cagcccgcgg cagggacgaa cctgccgaac ccggttcctg gaactgaagt
     1321 cgattccaac gggttggtgc agttcaccat taccgaagtg gaggtcaccg gcacggatgg
     1381 aacaatcctg gcggaagtgg atgtacagtt ttcgccgccg tcaatgggcc aatcgaggct
     1441 ggtgggaatt ccccttgtca gcttgcagcc cacagttctc tccgctgggg gatcgctagc
     1501 tggcggtcag acgctctact atgccgtaac ggccacggac agtaacggcg aagagaccag
     1561 cccctcgttt acgatacggg cgattattcc gcccgggagc tcgacgaata gcgtacagct
     1621 caacgggctg agcttcactg ctggtacggc ttcgtttaac gtctatcgcg gctcttttcc
     1681 tacacagctc taccgcatcg cgagcggggc gtccccggcg ccggcgttta cggatacggg
     1741 cttccccgta cagttgacgt cttcgccgga tcccttctat gaccacgcga atttctattg
     1801 gcggatggag gagaccgaag agaaattcgc cacgatcatt gcgctcaatc aagtcggcga
     1861 cgccggcctg aatctgacgc ctaacgctta catcgggcac gtggtgcggc tggtcagcgg
     1921 acaaggcgcc ggccaggagc ggaccgtcgt cgccaatacg gccacgacga tcacagtgga
     1981 tcgaaactgg gacgccatgc cggacgcgag cacacacttt gtagtgagcg aggccgcctg
     2041 gcattttggg gcgcgagccc gcagcagccc cgcgcgattt cagattccca accttaaagg
     2101 aagcgtcgtg cagatcaccg gcaggtcggc caacgtcaac aacattgagt cgccggagcg
     2161 gctcgcggtc gtgtcgcgat ggaagattgg gggcggagga accggggtgg ctgacgttgg
     2221 gccgccgccg gcgccttctt tcgggacatc ggttcacggc gacggcacaa tccgctttct
     2281 gggcatcgcg ttcggcgaac tcgccaacac gcaggggatt acttccggca ctttcctctt
     2341 gcattacaga gacgagttgg aaggtatcag cgcgatccaa ttaaacgccg gaatcgacgc
     2401 cagcgcgacg acagtcacgc tgaacgctcc gggcggcgca acagccggcg atctcataca
     2461 gttgggctcg gagatcatgc aggtcctcga ggtgcagaac gcgggcctgg cgtacagcgt
     2521 gagccgggcg cagcaaggca ctccggctgg cccgcacgac gccaacacgg ctgtgtatcg
     2581 tttgcagagc cgcacgatcg tcgtggcatt cgagaagaac ttcttctcaa cgccggaggc
     2641 aggttcctgg agccacagcg agtgggcgcc cgatatccgc ctcgccagcg tcgagttttt
     2701 tgtgaccaac gcgttcggcg ccagccctac ggcggtaaac acctatacgc agcttgccgg
     2761 aaacggccag agaacgctgc ggggcggcca gcttgatctt caggtcgaag gcgtgctggg
     2821 catacagagc gacgccgtcc ctgaaatcac cgtaggacaa gcggcctcgg tgtcgagcgt
     2881 ctcggcgcgc gtcgagctgg cccccgccgg cgccgacata actcttgtgg tgaaggtcga
     2941 tggggccgcg tgggcgactt taaccattcc tgacggccag ttgcaggcga acggcataga
     3001 cggcgccgag ctgccgccgc taatgaccgg ctctatggtc agcatggata tcacaggcgt
     3061 gggcacgacg tttcccggac agcgcctggc ggcgagcatc cggctttagc atctgaaaag
     3121 agcgcctagc gcggaaaaat gcccgaaaaa atctacaagc tacagccgca tcgcacgatg
     3181 cacctgcgcg gcttcgaccg ccggggcgcg gccgccgcgc tctggggcgc atcggacgcc
     3241 ggtttcacga tgagcggagt gttccgtgat cccgcggatt tctgcgtgct aatcctgttt
     3301 gacgccgacg atgttttcag tcacccgcgc tggcgctatc tgcccgactt tgatttcacc
     3361 ggcatcacgt tgcaattcga cgtgacatac acgaacttgc agccgctcga ctcgccgaag
     3421 tttccttcaa tcgactggga caagctggat atcatcaagg ccgatggagc cacggcgcag
     3481 gtgaagctgt gggaccacgc ggcgaagcag agcggtgggt tctccgcagc cagcctcact
     3541 tacacgctgc acggcgtccc ggcggctttt gaccgcgtga cgctgtggta tcagaacctg
     3601 gccttcgact acatcgtgcc gggggcgaca tcggtggatt atcagttttt tgccgccgga
     3661 acgggaacgg tccactcgat cactatcggc acgaacacgt actcttacac cgagaccgcg
     3721 ggcgacagca gcccggcggt ggcttcggct ctcgccgcgg cgatcaatgg cgtggatccg
     3781 aacgccgcgg cgagcgccag cgcgaaccat gtgatcctca cgccgactct cgacaacgga
     3841 tcgagcgtgg catgcagcgc cactgacggc aacagcctgg caactctcta tcaagtgcag
     3901 gcatccacgg tgctccggtc gatccgcgac cagatcaacg gcgcaaattg gccggcgcaa
     3961 ggcgcccccc taggtctgcg cgcggcggtg agcggatcga gcctggtggt tactgcggcg
     4021 cgctatggca cggtgaacac cagcgggacc acggtgacct gggcaagcgg agagaagttc
     4081 accggcatcg acccggccgg aacaatccag atcggcggcg cggtctataa catttccagc
     4141 gtggacagcc ccacgcagat cacgctcgct gcaagcgcag gcgcgcagag cggcgccgct
     4201 taccttgccg atcgcggcgg cgaggacggc aatatgatcg cgctctgcga gctgcacaag
     4261 aacgacaatc tttacttcac gccccagggc gccgctcaaa tgaccggcgg cgactccgcg
     4321 gcaacctggc gggtgacgct ggacttctcc tccctgggca ttaccagtat ccggcagatg
     4381 tggatgacct ttgcgccaca gttgaccaac gccggagcct acagcccgca ggaatggaca
     4441 gcagtgttca gcaactggac ggtgagcgac gccaatttga aacgggaact gaaggtcgcc
     4501 gggccgggat ccgtgcgggt gggcagccag gattcgtggg cgaactacac cggctcgggc
     4561 tggagctccg agtcgggttt ttactggctg ggtttcgcga agcgcaccgc gcaggcaggg
     4621 gatagcgtcg ccgtggaata ccattgccag catacgcacg atctgtacat cggcacttct
     4681 ctttacaaag atcgcgggat ttttgcggcc gttttggacg gcggcggcgc gacggacctt
     4741 gattgctatc tcgatgccga gcccgctgtc gtgacgcggc gcctagtgcg cagcggcgtg
     4801 cccgccggca aacacacgct caccttgata ctgaaggacg cgaaaaacgc cgattccagc
     4861 gggttcaatg cctattttga ctacctggag gcagccgttg cctccgacgt tccggatgcc
     4921 ccgcaggttt acggcgaccg cagcgtagca accgattttg acaccgacca cgcctataag
     4981 ctttcgcccc agcggctggt gtggatgatc ggcaagagcg ggctgcatgg agatatcgac
     5041 cactacgccg gcgttttttg gtggaaccag cgcaagcgcg taggcggcgc gttcccgtcg
     5101 ctcacggtca ctttcagcgg gacctgggcg gatggcgatg cggcgttcct gcaaatcggg
     5161 ggcaatgcgt tcaacgacgg cgccggcaca gtgatgggca aaagcgtatt tccggccgac
     5221 acaaccgaaa cgatcgcggc tcatttcgca agcttcatca acgagacgtt cgtgggcgta
     5281 tgggcgtcgt ccgcggccag tgtcctaact attacctgcc ggtcgcccga gtactcattc
     5341 acgttcttca caaataaaag cagcgctgct ggaacgatca gcgtgagcgg atcgctaagc
     5401 ggcggcgtgg agggaacctg gcagatcgat gatacaatca ctcccgtcat caaccgggcg
     5461 gcggccgact ggcacaggga ctatttcgcg gagatcgcgg cgaagggttg gacggcgacg
     5521 gccgctttct cgatggaact ggttgatccg ccggacaatc cgccgacggc ggcgtgggcc
     5581 cagcggttcc gggataacgc ggcggtgcaa accgacaccg gtttcgccag cctccagagc
     5641 acgcaatgcg ctttcagcgg cgcggtggcc gcttaccaga agcaggcctt tcaggagatg
     5701 gccggcttga tgaaattggc cggcttgacg ccctggctgc aattcggcga attcctctgg
     5761 tggtttttta ctaactacga cgcgggctcg aatcccggcg gcggcatggc ctattatgac
     5821 tcctacactg ccggccaggc gcaggcagtg ctggggcggc ccctggcgag ctttctgacc
     5881 cctggcgatg atccctcgca gaacaactat acagacgcga attttctgcg gagccagatc
     5941 aagacccaca tcgacgcaat ccgcagctat gtgctctcca cgcatcccga tgcaaaattc
     6001 gagctgctgt ggccgctgga tgtaaattat ccgccggcga agcgactgaa ccgttacgtg
     6061 aacttgccgg ctgagtatca gcaaccggtg ggaagcggcc tggacagaat caaaatggag
     6121 ggtctctcgt tcggcgcaac agagcggaac atcgatcatg cgcggcaagc gatgcggttt
     6181 ccttacactc cgcctctcgc ctgggccaaa tcggatgtgc gctacttgct tccctggttc
     6241 aatggcggat gcccctgggc gcgggaatat ctggccggct gccgtgagct gctgcccaat
     6301 atctccttct gggcatggga ccacctgaat ctgttcggct ggccgctgcc cctgccggcc
     6361 gaaacgcgcc ggagtgcgct ggtgaatacg tgatgcagcc gccctctctg gataaccgca
     6421 gagaagtccg gcggggccgc aaccggccgc gcccgccgtc ggattctgga tggcaaaatt
     6481 cgcaggagcg ccggcgtgtt gtccgggaag cggcgatcgc ttccaccgcc tatagccgtg
     6541 agtcagagcg caggctcatg caatccattt ttgacaattg cggccgccgg atcgccaagg
     6601 cgacgtgtgg ggatgcgacg cggaacgctt tgctggcggc catcgccgcc aacgaaagcg
     6661 gcggacggcc ccgccactac cggttttcct tcgaaaatta cggctgcctc ctcggtctgg
     6721 taaagggaga cgcctcgggc ctaaacggac tcacgcgccg gcaactggaa aagcggctcc
     6781 gggagccggg gccggagaca gaatccggcg ccctgctcaa gaaactggcg ggcctatacg
     6841 ggtacacaca aatccccggc tattactcga tcgtgtggaa cgcgtcgctg gaggcgctga
     6901 tggaagaaga gagtcatttt tcgttcgccg cggcgctgct ggaccggatt tgccggcaat
     6961 tccacctgga tccggagacc caagccgccg ccatcggacg ctgctggaac gcaggacacg
     7021 cctccgggcg cgccagctcc gcgctctatt cgtggagact ggtagaacgg atgaagttgt
     7081 accggggaat cgaagaatcc ggaacctgaa cgcgtaccgg aggccgcggc tcgcgtggcc
     7141 ggcgcatcgg aaccgcgccg gcagcgccct ggacgattat cgccctcgct caggttgcga
     7201 aaactggctg aatctttctc agacaggggc cgcccgaacg attcttttat actcgcgcga
     7261 cattgcccat gccgcgagcc ggcggcggga cgagcggccg gacggcgtgg tccgatttct
     7321 cgccgaaccg gacgctcacg agccgggaaa cgcccggctc ctgcatggtt acgccgtaga
     7381 gcgcctgcgc cacttccatt gtcgttttgc tatgggtgat caggatgaat tgcgtttgct
     7441 gactcatctg gcggatcagc cgggtgaagc gcacgatatt cgcttcatcg agtggagcat
     7501 ccacctcgtc gaggatgcag aaagggctgg gctgatactg gaacacagcc atgagcaagg
     7561 caatagcggt caaagccttc tccccgcccg aaagcagaag cacgttttgc agccgtttcc
     7621 cgggaggcga agccactata tcgatgccgc tctcggcgat gttggtttca tcggtaagac
     7681 gcatttcgcc gacgccgccg ccgaacagcg tgctgaaagt ctttcgaaaa ttctcgttaa
     7741 cgcgctcaaa cgcatcgagg aactgcttgc gcgagacgct gtcgatttcg ccgatggcct
     7801 gctgagtatc gcggatggat tcaaacagat ccttccgctg cgtatccagg aactccagcc
     7861 gctgctgggc ctcctggtat tcctcgagcg ccagaacatt caccggtccc aaagcctcga
     7921 tcttggcccg aaggctcgaa cattcctgtt cggctgcctc caattcgccg cctgcaagcg
     7981 gcgcttcccc cgcctcggcg atttcagcga cagggcgacc caactcccgg aggcag
//
LOCUS       DASREK010000265        22595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_294037, whole genome shotgun sequence.
ACCESSION   DASREK010000265 DASREK010000000
VERSION     DASREK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 22595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22595
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294037"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..787
                     /locus_tag="VFA54_14320"
     CDS             20..787
                     /locus_tag="VFA54_14320"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HZS52038.1"
                     /translation="MDAELDHGTSELSMESSRHLDECERCRRLYAWFRQTPAVAEAPP
                     HISASVLRNLPQSIQPVSPRRSTPAIASQLVGLFVLVAILPAILIGLAGLRQMSLLQL
                     ITMSIVLVGGLTALALSLGWQSAPGGLQRFSLTAAVGIAALAFLLCSALPFPWHTPEP
                     FYERGWLCLKHGLGLALVSALVFWLLLRRGAPMGRGFGTALGAVSGLAGITMLQLTCD
                     RQEGEHLLFWHGGVLVVATVCGLLIGKLFPRFSAKSA"
     gene            855..1205
                     /locus_tag="VFA54_14325"
     CDS             855..1205
                     /locus_tag="VFA54_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52039.1"
                     /translation="MRNLFKIFCCAGLVAGVASAAQVQGVLMDKMCSMKAAKEGQSAA
                     MGHELKCALMPPCQKSGYGVFTADNKFLAFDDAGNAQALKALQGSKKKDDLKVTVNGN
                     VQGEMIKVTSLQLQ"
     gene            1308..2198
                     /locus_tag="VFA54_14330"
     CDS             1308..2198
                     /locus_tag="VFA54_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HZS52040.1"
                     /translation="MANLSTDKFRVGLVQMSCALDPNQNLEKAIWRIREAAGRGAQII
                     CLQELFRSQYFCREENAELFALAEPIPGPTTECLGKIARELGVVIVASLFERRTAGLY
                     HNTAAVIGPDGQIVGIYRKMHIPDDPLYFEKFYFTPGDLGFIDFDTHFGKVGVLVCWD
                     QWYPEAARLVALRGAQVLFYPTAIGWHPAEKREHGQAQHDAWKTIQRAHAIANGLYVA
                     AVNRVGHEGPAGGGLEFFGGSFICDPFGVVLAEASHDREEILYAECDLARLEEIRRNW
                     PFLRDRRIDTYGPLTSRWIG"
     gene            2245..3261
                     /locus_tag="VFA54_14335"
     CDS             2245..3261
                     /locus_tag="VFA54_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZS52041.1"
                     /translation="MPAEWEPHAATWIAWPHNRGDWPGKFSPIPWVYVEIVRRLHLSE
                     RVRILTTAPERPARMLCRAGVDLSRIEFIPFPTDRSWTRDYGPMFVCSEGKLAINDWR
                     FNGWAKYSNWKRDVRVAARAARRLKTALFQPGLVLEGGAIDVNGQGTLLTTEECLLSE
                     TQQRNPGKSREEIEAALSAHLGVRHFLWLRKGIAGDDTHGHIDDIARFVGPRRVVAAV
                     EENASDANYVPLQENLELLRSMKDQDGNPLEVFALPMPEPLYFDNRRLPASYANFYIA
                     NDVVLVPTFDDPRDRAALGVLAELFPDRKVVGIHAVDLVWGLGALHCLTQQEPACGSP
                     SQRL"
     gene            complement(3383..3745)
                     /locus_tag="VFA54_14340"
     CDS             complement(3383..3745)
                     /locus_tag="VFA54_14340"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52042.1"
                     /translation="MGFFRKEVIDFALDHETRRAVEEQRAAIAADPADPRPYFRLAAL
                     YRMQWRQEEALGLLLEAVRLDPRFAAAHAALSEIYAARNDRSLAGRHAEAAAALGDSS
                     ALERLRRYGLLPPPGESS"
     gene            complement(3834..5051)
                     /locus_tag="VFA54_14345"
     CDS             complement(3834..5051)
                     /locus_tag="VFA54_14345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS52043.1"
                     /translation="MSVAAIVKIALRALGRNKMRTALTMLGIIIGVAAVIAIVGISQG
                     ANEQMQQAISDMGANLLFISPGSPNVGGVRAGGGAAATLRVSDMAAIERQVKLVRLIS
                     PGVFDSAMTVYQNRNWSTRVQGVAPSYFEIRMWPVSAGSPFGDAEVERAADVCVLGQT
                     VVDNLFQGEDPVGKMIRVKNLPMTVMGVLAVKGPDPGGRDQDDTILMPYTTVQKKLRG
                     IDYIHFIQASAISEQATAAAQTQIEGVLRERHRIRPGQPDDFTVRNMADFAEVANQAG
                     SVMTMLLASVASVSLIVGGIGIMNIMLVSVTERTREIGIRMAIGATEQDVLRQFLIEA
                     VILSMLGGLAGIVVGIASVIGMAQVLRWPTTVSITALLSSMLFSAGIGVFFGFYPARK
                     AAQLDPIEALRFE"
     gene            5176..5607
                     /locus_tag="VFA54_14350"
     CDS             5176..5607
                     /locus_tag="VFA54_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HZS52044.1"
                     /translation="MPQEKDAAGKPAKPPRPPLNLWIAPVALGWLLPGGGHFYLKRWN
                     RGALLLFAVSGMFVFGLLMRGRMFEPGDTDLFNIILNYGGFVGDLANGALYFLATWFG
                     YSQPDMPGAAPDYGAKFLVCAGLLNILAMIDAYEIAVGNKS"
     gene            5607..5789
                     /locus_tag="VFA54_14355"
     CDS             5607..5789
                     /locus_tag="VFA54_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52045.1"
                     /translation="MHWNPTHFEASFLFALFTSIVFGVVGKRTDRERLHYGLYVFGCF
                     MAAIFGLAWLMYFGHG"
     gene            complement(5932..6897)
                     /locus_tag="VFA54_14360"
     CDS             complement(5932..6897)
                     /locus_tag="VFA54_14360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52046.1"
                     /translation="MTPSEPKQAYSRDDIRRILKVSDARLRSWERIGLFEPRQEFSFS
                     DLIALKTLQKLRENRIPPDRIRRALTSLSKKLAGVERPLTELKIISDGKKIAVDLGDG
                     KMEALTGQLLFDFDASELRRPVAELKRREHQSRETNERESEHWFQIGLQLEESGAPPE
                     EALRAYARSLELNPHAAGACVNMGTIYYQLRNLAEAERCYRRSVEIDPEYALAHFNLG
                     NIYDELGRSEDARAHYLMALRLRPDYADVHYNLALLYEKNGEAMRAIRHWRSYLKIDS
                     SSPWAGIARQQLEGLLRVTPGGRRSPSASADRLFANLGDPQSSKD"
     gene            7005..8036
                     /locus_tag="VFA54_14365"
     CDS             7005..8036
                     /locus_tag="VFA54_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52047.1"
                     /translation="MAGYEPADVNIAHPNDTADVEQESSDFRAGQDYMHLTATLAPRP
                     APLIFNAEDNCCFRAPMDKEGVYDSIKAFYAFYGKTESLAWHENLDPGAHNYELDNRE
                     QSYRFLAKHSGLSTLDREIPADNEVRSEEELAVRLPKDNLTILELACRFAANIHRDTL
                     LPQDAQTRLQSIVRYKPASLERAWMVDGSKNKGVESRSYQLLFNNGLTAAAVWFKSMT
                     APDNARRARRPQGSRPLVREASSHPWLAGAITPSLFSKTGSRKGMPSLVVIFNSPIKF
                     RDASPCSARISTRSSISIPSRRSALSALFSKRRIRRLPRHRLLLQKLYSLQHLLRQVG
                     FAELSIRET"
     gene            complement(7941..9290)
                     /locus_tag="VFA54_14370"
     CDS             complement(7941..9290)
                     /locus_tag="VFA54_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52048.1"
                     /translation="MRTGIIFLLLCCSLKAADCEPSQLTEKERLSKFQELDRQAQSAF
                     QQNRYSAAAKYYREAACVAPKSARAFYGLGTAEAAAGNFSAARQAFETADALLPDNML
                     PLTMLVRVNVAMKDIERTKEVLRAAAKRFPKIPDLHAGLARFLAESQLLDLALAESLR
                     AGDSGATGAESLVALAALENTVGAFHDAIQTASAVENDPHAPEAIKASASGVAGLSYE
                     SIGRRQEAIDHLKLAVERAPAQENSYLALAFIYEKAHEFGEAVEVLERARARMAGSSA
                     LLLALGNNLVWAGEYQRGEQVLNDFLQKDPGAAEAYVRLAEAYRNTNRTALEIEALQK
                     LARIKPDYPMVHVLTAEAMLRMDPVDYNGVLQELAKAEKAAPNDADIFYLRGKVYVAM
                     NRLSEAVTALKRSIDLRPTEARPYYQLGLTYRKLGKADLAKEMLQRVEFLKQQPVAR"
     gene            complement(9287..10288)
                     /locus_tag="VFA54_14375"
     CDS             complement(9287..10288)
                     /locus_tag="VFA54_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HZS52049.1"
                     /translation="MSEPRNLSFPLQSIEGVLTPPDLFFVRDHFREPDVSLRTWRLRI
                     DGRVAKPVELSFADLVESPARKLEAVLECAGNPAGGSAVSNAMWEGVPLANLLRDAGM
                     AQDAETVVLEGADSGSLMDNSPALPYCQVVPVAKCMREESMVAFKLNDRFLPRKNGFP
                     ARALFPGWYAMDSVKWLRRIIVLGANERAADFEASGMNAVYNRIVAAAGGERKVTRLS
                     EVAVKSAIAWPADEAKLPAARHIIRGFAWTGGGRIRTVEVSHDGGRAWSPARLEGPSK
                     PFTWVRWRYLWDAKPGEHVLLSRATDESGRQQPLARDPARKDGYELNFCAAVRCYVA"
     gene            complement(10371..13562)
                     /locus_tag="VFA54_14380"
     CDS             complement(10371..13562)
                     /locus_tag="VFA54_14380"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS52050.1"
                     /translation="MAKRGLLFSVGCLFLALSAYGAEPTGTIAGTITDPSGAAVVGAR
                     ITATNLNTGLSRETTSTADGGYVFPLLPVGIYSIAVEAPGFRRFEQKGIDVRADQNTT
                     VAVKLEIGATTETVSVVANGELVETRSSTLSKVVTTQSVSELPLNGRNAAALVLLAPG
                     TADLTAGNARGSGDTQQTATYPGAQSISSNGARADDINYNLDGGSNQDHYTNVNNPFP
                     NPDAVEEFSVQTNSYSAEYGRGAGAIVNVVTKSGTNSFHGSAFEFLRNGDLNARNFFA
                     ASHDLLKRNQFGGALGGPIKKDHLFFFGTYQGTRLRNVSLANTATVLSDAQRQGDFSG
                     VQRQLVDPITKQPFPGNIIPTSRLDPVAVKLLNYIPHSTRPDSLITYDLPQIDNENQT
                     MGRLDYNLTSHRLYGRYFFSEYTHAPTVGSVNLIKARNGYSDRDQSVSFSDTYNIRPN
                     LLNNFIFSFNRINGSILSGAPFSFPSLGIPIATSNPPEMVISVSGYFSISTGHPGHFN
                     RQNFHFADSLHWIKGSHEIAIGGDLMKMQVDLINTFRQNGNFRFRGTSYSGDPRSDFM
                     LGAVDRFIQGGGEYAARRGTLGSLFIQDNYRVSPNLVLNLGLRWDPFYPYGDTLGRTE
                     CYRPGLISKRFPNAPPGYLYEGDPGCPTGGSEPVPWNFAPRVGFAYNIGGDRKTVVRS
                     GFGLFYQPPFVEAYNNMVDSAPWSPQVQLFNVPFDNPYANYPNPFPAQFAPFIPPSNV
                     QFIIPPSLAVSYTPDWKPGRTLAWNFTVERQLHNDLLFRVGYAASKGTHLGYNTDVNA
                     PLPSPTATADNESQRRPNPYFQEVVEDVSGGNSIYNSLQVSMEKRFSHGFTVSANYTF
                     AKTIDEVSYLTDLCGVNVINPYNVRAYRAVADFNVPHRFVLNYLWQLPSPSQGVAKAL
                     LGGWQTSGIWNWQSGFPLNITSADDRGLVGIGNDLADVVKPPTYTSGPLGARILKWFD
                     TSAFTQARLGTFGNAGRNILIGPGTFNIDFSLHKFFTIRENLKLQYRAEFFNVLNHTQ
                     LNNPDTAVPDSNFGRITSARAPRIIQMALKLIF"
     gene            complement(13938..16739)
                     /locus_tag="VFA54_14385"
     CDS             complement(13938..16739)
                     /locus_tag="VFA54_14385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZS52051.1"
                     /translation="MFLSAGAWLAFVGAVASLAWMRRGAAEPYRAALESVPVIYFALW
                     TTCGYFVFIGLGLAALLKPYRVPLRSIRPAVVVDTLLLGFLTLDWEFSIAAAARLASA
                     RSISFGMAFFPLTLALLCAGSLTASRHRREPGPWKRTYRDLALAAACQSASRTILALS
                     YGSLAPSTFATPAAADVLQTAAFFLLASAARHPTAAPAASPKEGSTAEPPIISVWREL
                     SIPFLTLLGIGGIPLIEKFFGSRPGAFAPMVELRRETLLVSLGVYVVLVLARQFLVQM
                     ENRDLALDLQQESARLRLLLDNIHDAVITEDLRGRLVFANDRFLHMFGLRRDQIGGLR
                     LDACIHPEDRRLRHDWHEPLFADSEGGARFEFRGLRSDGTTLYLESSVVPVCLRGMVL
                     GYQSVIRDITERRKAEERQRELVQRLEFLVSHMPLGCIVFDLDFRILEWNSSASRIFG
                     WSAPEVFGRNGLELLVAPESRLEIVAAWKELQLSQRSDHRVTQNLTKDRGVIECEWFN
                     TSLIDETGAVVAVASMVQDVTERKNLEAQLRQSQKMEAIGVLAGGIAHDFKNLLTVIV
                     GNISLALMRLGAAHPAGRGLQDAEKAAERAAELVQQLLGFSRKSPIRPRPIDLNASVR
                     ESVDLLRRTVDPRIIIETSEQPDLWLVEADRGQINQILLNLCLNARDAMEDGGCLSVS
                     TANLVIGADYCRSRPEARPGEFVCLRVADTGAGMDQATLSRIFEPFFTTKQVGKGTGL
                     GLSMVYGIVKQHGGWITVESQPRCGSVFSVFLPRSPKAAEEDAPRVTPSDTKGSETVL
                     LVDDEEMIRTLATDILERRGYRVLQAQDGEEALEVVRQDLPGPPGRIDIVLLDMTMPR
                     KSGRDTLAALREVAPELPVVLASGYSADGNEDLAALGARAFIQKPYRPEDLLRTLRAV
                     LDSALRRQTAAEEHFPVQE"
     gene            17019..19766
                     /locus_tag="VFA54_14390"
     CDS             17019..19766
                     /locus_tag="VFA54_14390"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZS52052.1"
                     /translation="MRFFGYNRESMLLTGPPGSGKTSRVLAELISALARGQDPRPILI
                     VPTASMAEHTQHRIARQGILVPPKAILPLQSYIEQLTPASREASAAAEQVLLEAALRL
                     VDTSEFGPAAEYPGFQRILLDTIQEFWSAGADPGAFLEKAEYKPYGAPFLRIMREFEN
                     QLHAAGYVTRAERFRMAAAELGRQRLKSVFLDGFFHFTAMEQELVKALAGTAEKILIT
                     LPETGAEQARQALLAMGLPEERLTELRRLSKPVLVRAFSPEGEIEDVAGRILTDQKKT
                     GRPFYEYGVILRDPEAYAPIVAVVFERFGIPFRLKQPQPLTSHSAIRYLTGLLHLAAG
                     GFDGEAALAVMKLAPSGIAFSREMDGYEFEVRKSLPGRGLPFLLALAERRPLVLKWIG
                     KLEQLADWNRERLAPADWASRCEGLARTWLRAPVVKDGLGNSPVLELRAWASAADAWR
                     ASAREASELLATCGTKTADLPQYLEMLSKVLRAAPLRVADRRRNVVQVLSVYEARQWE
                     LAVVFVCGLIEKQFPRYHPQNLFFPDVQRRALSAQGLVLRTSDDRDREERFLFDLALT
                     RATEQVYASYPAHDDKGAETVRSFFLEPWKEQEVPAQEARFAGGAQVPSGPVVWAQKA
                     VLRDPGLLQVLGEQPSEFSPSGLQKYIQCPFQFFAARILRLQEPPPKPDERLDAMLQG
                     KIIHQTIAQWMNSRDRPIREVGEEVLRQTCDEEHIRLNFRAEAMKLQLLADLERFAES
                     EAAASAPAGYQAGEPEKSFRYTLAAGAGAPVCITGRIDRHELSSSAAAIVTDYKYSRA
                     DRITELVKQHEKGEALQGWLYLLGLKNELGVQPAGLVFYGLRKEICRKGWYVPELAPA
                     GDGLEEASAAQFQQMLGRAEARALEIVQDLRKGRIEVAPRDRRLCRDQCPYLDVCRVD
                     L"
     gene            complement(19702..20052)
                     /locus_tag="VFA54_14395"
     CDS             complement(19702..20052)
                     /locus_tag="VFA54_14395"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZS52053.1"
                     /translation="MIDRPIRFDIERLVEFRVADAKGATPHQGRTINISSSGVLFHTD
                     QQLAVGRKLEMVVHMTQTSPDGVDVNLYLLGTVVRSGPGWAAARVRKHQILPTNPPGT
                     RPSKDTGRGTAGGP"
     gene            20152..21060
                     /locus_tag="VFA54_14400"
     CDS             20152..21060
                     /locus_tag="VFA54_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZS52054.1"
                     /translation="MAFVPVLHSVSYAGAWPGQACLSLEQFLAKAAELGFPAVMLMAK
                     RPHLSVLDYDRKSCERLRRLMDDKGIECRVIAGYNNFSADIEHGDVPNREIQIQHVTE
                     LARMASDLGAGIVRIFTSYESPAASYSTLWQTTVDSIRECARRAAKYQVTIGVQNHHD
                     LAAHYESQFDLIGAVGEPNCRAIFDAWAPALQGTDLGKAAQIMAPITCHTTAADYKRR
                     PRFRYHPKLVNYEKQIDWVQAAPMGEGFIDYTGFLRALQRGGYAGGVAYEMCSPLAGG
                     GTIENLDCYARRFLEFLNSVEFETTK"
     gene            complement(21423..>22595)
                     /locus_tag="VFA54_14405"
     CDS             complement(21423..>22595)
                     /locus_tag="VFA54_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HZS52055.1"
                     /translation="KGGVGKTSIAAATGLELARRGYRTLVMSVDPAHSLADAFDLDQS
                     LFSAATGLPYSINKRLAVQEVNIQQEIKRHWQEIWSYIVSLLQATGITQVEAEELAIF
                     PGMEEVSSLMYVNQYRRRKEYDVVVVDCAPTAESLRFVSMPTTLEWYMKNIFHLERTL
                     MKAVRPVANRVSPVKLPSDSYFSNIQKLFERLEGIGELLEDSRVVTVRLVTNPEKMVL
                     RETQRAFVYFALHGMTVDRIIVNRVLPAAVQDRYFRDWQRSQQKTIEQIEEYFAPVPV
                     SRVPLFAHEVVGSARLAELARVLYQEPDIDPAARSRQEAPYHFEKNGSGYRIRLHLAF
                     AAPGEVSVFKKGDELVIEVGAVRRHVGLPTTMAALEPRKAKLEEGILTVELVEGRK"
BASE COUNT         4471 a         6800 c         6925 g         4399 t
ORIGIN      
        1 cagcagagat tgcagcctgg tggatgcaga actggatcat gggacatccg agctttcgat
       61 ggaatcgagc cgacacctgg acgaatgcga gcgctgccgc cggttgtacg cgtggttccg
      121 tcagacacca gcggtcgccg aagcacctcc gcacatatcc gcttcggtct tgcggaatct
      181 gccgcaaagc atccaacccg tatcgccgcg gcgttcgacc ccggcgatcg catcgcagct
      241 tgtgggcttg tttgttcttg ttgcgatcct accggctatc ctcataggac tggcagggtt
      301 gcggcagatg agtctgctgc agcttatcac catgagcatc gtgctggtcg gcggcttgac
      361 cgcgttggct ctgtcactcg gttggcagag cgccccgggg ggccttcagc ggttttcgct
      421 gactgcggcg gttggaattg cggcgctcgc gtttcttctc tgctccgccc taccgtttcc
      481 ctggcatact ccggagccgt tctacgaacg cggatggctg tgcttaaagc atggtctcgg
      541 actggcgctg gtttccgcac tggtattctg gttgctcttg cgccgcggcg cacccatggg
      601 ccggggcttc ggaaccgctt tgggagccgt ttccggtctt gcgggcatca cgatgctgca
      661 gttgacgtgc gaccgacagg aaggcgagca cctgctgttt tggcatggtg gcgtcttggt
      721 cgttgctact gtctgcggcc tgctcattgg caagctcttt ccgcgcttct ccgcaaaaag
      781 cgcgtaactt tctctgttcg agagcgaagt acttagcggc cgtcgaggcc aatcgcaaag
      841 gagaaacgaa gtcgatgagg aatttattca agatattttg ctgtgcgggt cttgtggccg
      901 gcgtcgcctc cgcggcccag gtgcaaggcg tcctgatgga caagatgtgc tccatgaaag
      961 cagctaaaga gggccagagt gcggccatgg gtcacgagtt gaaatgcgcg ctcatgccac
     1021 cctgccaaaa gagcggctac ggcgttttca ccgccgacaa caagttcttg gcgtttgacg
     1081 acgctggcaa tgcccaggcc ctgaaagcgc tccaaggctc aaagaaaaaa gacgacctga
     1141 aggtaaccgt aaacgggaac gttcagggcg agatgatcaa agttacaagt ctgcagttgc
     1201 aatagtcagg ccgggaggcg gctcagtagc gtgcggccgc ctccttcttt tcagactcct
     1261 tccgaacgcc atttgcggct ttgccgccct gcccccacaa taaagacatg gcgaatctga
     1321 gcacggacaa gttccgtgtg gggttggtgc agatgagctg cgcgctggac ccgaaccaga
     1381 atctcgaaaa ggcgatctgg cggattcgcg aggcggcggg gcgcggcgcc caaatcatct
     1441 gtttgcaaga gctgttccgc tcgcaatact tctgccgaga agagaatgcc gagctgtttg
     1501 cgctcgccga gcccattcct ggccctacca ccgagtgcct cggcaaaatc gcccgagagc
     1561 tcggcgtggt gattgtggca tcactgttcg agcgccgaac cgcgggcctg taccacaaca
     1621 ccgcggccgt cattggcccg gacggccaga tcgtcggcat ctatcgcaag atgcacatcc
     1681 cggacgatcc gctgtatttc gaaaaattct acttcacgcc cggcgatcta ggcttcatcg
     1741 atttcgacac gcatttcggt aaggtcgggg tgctggtctg ctgggatcag tggtatccgg
     1801 aggcggcgcg gctggtagcg ctgcgcgggg cgcaagtgct cttttatccc acggctatcg
     1861 gctggcatcc ggcggagaaa cgggagcacg gccaggccca gcatgacgcc tggaagacca
     1921 tccagcgcgc gcacgcgatc gcaaacggtc tttatgtcgc cgcagtcaac cgggtcgggc
     1981 atgaggggcc ggctggaggc ggcttggagt ttttcggcgg ttcgtttatt tgcgacccgt
     2041 tcggtgtggt gctggccgag gcctctcatg accgggagga gattctttac gcggagtgcg
     2101 acctcgcccg cctggaagaa atacgccgca actggccgtt tctgcgggat cgccgcatcg
     2161 acacctacgg cccgcttact tcgcgctgga tcggctgatt cccgcatgcc cttgtccaaa
     2221 tcgccagcga gccttggtta ccgaatgccc gccgagtggg agccgcacgc cgcaacctgg
     2281 atcgcctggc cccacaaccg cggcgactgg ccgggcaagt tttcgccgat tccgtgggtc
     2341 tacgtggaaa tcgtgcgccg cctgcacctc tccgagcggg tccgcatttt gaccacggct
     2401 cccgaacggc ccgcacgcat gctttgccgc gcgggcgtgg atttaagccg gattgagttc
     2461 attcccttcc ccaccgaccg ctcctggacc cgcgattacg ggcctatgtt cgtttgcagc
     2521 gagggaaaac tggcgatcaa cgactggcgc ttcaacggat gggcgaagta ctcgaattgg
     2581 aagcgcgatg ttcgagtagc cgcccgcgcg gcgcgccgtt tgaagaccgc tctgttccag
     2641 cccggcctgg tgctggaagg gggggccatc gacgtcaacg gccaaggaac gctgcttacc
     2701 acagaggagt gcttgctaag cgagacccag cagcggaacc ccgggaagag ccgggaggaa
     2761 atcgaagcgg cgctctccgc ccacctgggc gtgcggcatt ttctgtggct gcgcaaaggc
     2821 attgccggcg atgacaccca cggccatatc gacgacattg cgcgctttgt gggcccgcgc
     2881 agagtggtgg ccgctgtaga ggagaacgcg agcgacgcca actatgtccc gctccaggaa
     2941 aacttggagc tgctgcgcag catgaaagac caggatggaa atccgctcga agtgttcgcc
     3001 ctccccatgc ccgagccgtt gtatttcgac aaccgccgct tgccagccag ctacgcgaat
     3061 ttttacatcg cgaatgatgt agtgctggtt cctactttcg acgatccgcg cgaccgggcg
     3121 gcgctcggcg tcctggcgga gctattccct gatcgaaagg tcgtaggaat acacgccgtt
     3181 gacttggtct ggggcttggg cgccttgcat tgcctcacac agcaggagcc ggcgtgcgga
     3241 tccccgagcc agcgtctttg atttggcgag gccacgagct cggccgccgg cgaaattttg
     3301 cgaattcaga cgcgcgagtc ccccgcggct cgaaggctgc ggcggcctca aatctccgat
     3361 tcgcgaagat tactgccttg gcttatgagc tttcgccggg cggcgggagt agaccatacc
     3421 ggcggagtct ctcgagcgcg ctcgagtcgc ccagcgcggc cgccgcttcc gcatggcgcc
     3481 cggccaaact ccggtcgttg cgcgccgcat agatttccga cagggcggcg tgagccgcgg
     3541 cgaaacgcgg gtcgaggcgc acggcttcga gcagcagccc gagcgcttcc tcctgtcgcc
     3601 actgcattcg ataaagagcg gcaagacgga agtaaggacg cggatcagcc ggatccgccg
     3661 cgatggccgc ccgctgctct tccacggcgc ggcgggtttc gtgatcgaga gcgaaatcaa
     3721 taacttcctt gcggaaaaag cccacgaaaa acgctccttg cggccccgga atttcagatg
     3781 aggggcgccg cgtccgacgc tcgcaaagca agcccgggcg aagcctctat ccgctactcg
     3841 aaacggagag cctcgatagg atcgagctgg gcggctttgc gagccggata gaatccgaag
     3901 aacacgccga tgcccgcgga aaacagcatg gaactcagca gcgccgttat cgaaacggtc
     3961 gtcggccagc gcagcacctg agccatacca attaccgagg caatcccgac gacaatcccc
     4021 gccaggcctc cgagcatgct caggattacg gcttcgatga ggaactggcg gagcacgtcc
     4081 tgctcggtgg cgccaatcgc catgcggatt ccgatctcgc gggttcgctc ggtcacgctg
     4141 accagcataa tgttcataat gccgatgccg cccacaatca gcgacaccga ggcgacgctg
     4201 gccagcagca tggtcataac gctgccggcc tggttggcga cctcggcgaa gtcggccata
     4261 ttccggacgg tgaaatcatc cggctggccc ggccggatgc ggtgccgctc ccgcagcacg
     4321 ccttcgatct gcgtctgcgc ggcggccgtg gcctgctcgc tgatcgccga agcctggatg
     4381 aagtggatgt agtcgatgcc ccgcagcttc ttctgcacgg tggtgtaagg catcaggatg
     4441 gtgtcgtcct ggtcgcggcc gcctggatcg ggccccttga ctgccagcac gcccatgacc
     4501 gtcatgggca gattcttcac gcgaatcatt ttgcccaccg gatcctcgcc ttggaataga
     4561 ttgtcgacca cggtctggcc gagcacgcaa acgtctgcgg cgcgttcgac ttccgcgtcg
     4621 ccaaaggggg atcccgccga aaccggccac atccggatct caaagtaaga aggcgccacg
     4681 ccctgcacgc gcgtggacca gttgcggttt tggtaaaccg tcatggcgct gtcaaagacg
     4741 ccgggcgaaa tcagccggac gagctttact tgccgctcga tcgccgccat gtcggatacc
     4801 cgcagagtcg ccgcagcgcc gccgcccgcc cgcactccgc ccacattcgg gctgcccggg
     4861 ctgataaaca gcagattggc ccccatatcg gaaatcgctt gttgcatctg ttcattggcg
     4921 ccctggctga tgcccacaat ggcgatgacc gccgccaccc cgatgatgat gcccaacatg
     4981 gtgagcgcgg tccgcatttt gttgcggccg agtgcgcgga gagcaatttt gacgatggcg
     5041 gcgacgctca tgcggttatt tcaccataaa aacacactgc ctgtatctat aggggcgtcg
     5101 ggtttcgcgc gccggttacc ggcagttctc cagatccagc cgggctcggg cagccgtgtg
     5161 gtaaattaga atttcatgcc tcaggaaaag gatgcggccg gcaagccggc gaagccaccg
     5221 cggccccctc tcaatttgtg gattgcgcct gtagctctcg gctggttatt gcccggcggc
     5281 ggccattttt atctgaagag atggaatcgt ggggcgctgc tcctcttcgc cgtgtccggc
     5341 atgttcgtgt ttggcctgct gatgcggggc cgcatgttcg agccgggcga taccgacttg
     5401 tttaatatca ttttgaatta tggcggcttc gtaggagatc tcgccaacgg tgcgctttac
     5461 tttctcgcca cctggttcgg ctatagccag cccgacatgc ccggggcggc gcctgattac
     5521 ggggcgaaat tcctggtttg cgccggcttg ctcaatattc tggccatgat cgacgcctac
     5581 gagatcgccg tcggcaacaa atcttgatgc actggaatcc aacccatttc gaagcctcgt
     5641 ttttgttcgc gctgtttacc tcgattgtct tcggagtcgt gggcaaaagg accgaccgtg
     5701 agcgcctgca ctacggcctc tatgtcttcg gttgcttcat ggccgccatt ttcggcctgg
     5761 cttggctgat gtacttcggc cacggctgat ttttgagatt ccgcgcgccc atctttctta
     5821 aaaatacaga cggcgccatc gtccggcccc gcgctcgggg gcggcgcttc cgcctgcgga
     5881 tcccgggcgc tttcatccgc aaatccccaa ggcgtcattg gcgccggtcc ttcagtcctt
     5941 cgaggattgg ggatctccga gattagcaaa gagacgatca gcggaagcgg agggagagcg
     6001 gcggccgccc ggcgtaacgc gcagcagccc ctcgagctgc tggcgggcga tgccggccca
     6061 agggctgctg gaatcgatct tcagataact gcgccagtgc cggatcgctc gcatcgcctc
     6121 tccgttcttt tcatagagca gcgccaggtt gtaatgcacg tcggcgtagt cgggccggag
     6181 gcgtagggcc atcaggtaat gggcccgggc atcctccgat ctgcccagct cgtcgtaaat
     6241 gttgcccaga ttgaagtgcg ccagggcgta ttccggatcg atctcaaccg accgccgata
     6301 gcagcgctcg gcctctgcga gattgcgcag ttggtaataa attgtcccca tattcacgca
     6361 cgccccggcg gcatgggggt tcagctcgag cgaccgcgcg taggcccgca aggcttcttc
     6421 gggcggcgcg ccgctttctt ccaattgcaa tccgatctga aaccagtgct cggattcccg
     6481 ctcattggtc tcccgagact ggtgttcgcg gcgcttgagt tccgcaacgg gacggcgcag
     6541 ctcgctggca tcgaagtcga acagcaactg tccggtgagc gcctccattt tcccgtcgcc
     6601 cagatctacg gcaatcttct tgccgtccga aatgatcttc agctcggtca gcgggcgctc
     6661 cacgccggcc agttttttgg aaagagaggt aagcgcgcgg cggatccggt cgggcggaat
     6721 ccgattttcc cgtaattttt gcagcgtctt gagcgcaatc agatcggaga acgaaaattc
     6781 ctggcggggc tcgaacaagc cgatccgctc ccaggagcgc aggcgggcgt cggacacttt
     6841 cagaattcgc cggatgtcgt ctcgcgagta cgcctgcttc ggctcgctgg gcgtcacgcg
     6901 gaaattctat cacggtttcc acgccgcaga gccctcagat ttgtccaaca caagatgatc
     6961 ctgaaacggg cattgattcc agcacgtcaa gctgtcgatg ccccgtggcg ggttacgagc
     7021 cggcggacgt caacatcgct cacccgaatg acacggccga tgtcgaacag gaatcatccg
     7081 actttcgcgc cggccaggat tacatgcacc tcacagcgac gctcgcgccg cgccccgcgc
     7141 cgctcatatt caacgccgag gataactgct gcttccgtgc gccaatggac aaagaaggcg
     7201 tgtatgacag catcaaggct ttctacgctt tctacggcaa aactgagtcg ctcgcgtggc
     7261 acgagaatct cgaccccggc gctcataatt acgagttgga taaccgcgag cagtcttatc
     7321 gatttctggc aaaacactct ggtctttcca cgctggaccg ggagattccc gccgataacg
     7381 aggtgagatc cgaagaggaa ctggccgtga ggttgccgaa ggacaacctc actatcctcg
     7441 aactggcctg ccgcttcgcg gccaacatcc accgcgatac actgttaccg caggatgcac
     7501 agacacgcct ccagtcaatt gtgcgataca agccggcgtc gctcgaacgg gcatggatgg
     7561 tcgatggctc gaaaaacaaa ggagtcgaat cgcgttccta ccagcttctc ttcaataacg
     7621 ggctgacagc cgcggccgtg tggttcaaat cgatgacggc gccggacaac gcaaggcggg
     7681 ccagacgtcc gcaaggatcg aggccattgg tccgcgaagc cagctcgcat ccgtggcttg
     7741 cgggggcgat cacgccgtct ctgttttcaa agactgggag ccggaaagga atgcccagcc
     7801 tggtggtaat cttcaacagc ccaatcaagt tcagagatgc ctcgccctgt tctgccagga
     7861 tctctacaag atcatcgata tcgattccat cgcggcgctc agccctgtcg gcgctcttca
     7921 gcaaacggcg aattcgcagg ttaccgcgcc acaggctgct gcttcaaaaa ctctactcgc
     7981 tgcaacatct ccttcgccag gtcggctttg ccgagctttc gatacgtgag acctagctga
     8041 tagtagggtc tggcctccgt gggacgcaga tcgatggagc gcttcagcgc cgtcactgcc
     8101 tcgctgagac ggttcatagc gacgtaaacc ttgccgcgaa ggtaaaagat atcggcatcg
     8161 ttgggcgccg ccttctcggc tttcgcaagc tcctgaagca cgccgttgta atccacggga
     8221 tccatcctca gcatcgcttc cgcagtcagc acatggacca tcggatagtc cggcttaatc
     8281 cttgcgagct tctgcaaggc ctcgatttca agcgccgtcc ggttcgtatt gcgatacgcc
     8341 tccgccaagc gcacgtatgc ctcagcagcg ccgggatcct tttgcagaaa gtcgttgaga
     8401 acctgctcgc cgcgctgata ctctccggcc cagacgagat tattgcctag cgcaagtagg
     8461 agtgcgcttg agcctgccat ccgcgcacgc gcgcgctcga ggacctcaac tgcctcgcca
     8521 aactcgtgcg ccttttcgta gatgaaggcc agcgccagat atgaattctc ctgggcgggc
     8581 gccctctcga cggcaagctt cagatggtcg atcgcctcct gccgccggcc aatgctctca
     8641 tagctcaagc cggcgacccc ggacgcggaa gccttgatcg cctccggggc gtgcggatca
     8701 ttctccaccg cgctggctgt ttgtatcgcg tcgtgaaatg cccccaccgt gttctccaga
     8761 gccgccaggg caaccaggga ttcagcgccg gtcgctccgc tgtcgccggc ccgcagcgac
     8821 tcggcaagcg caagatccag gagctggctc tccgcgagga accgagccag tccagcgtgt
     8881 aagtccggga tcttcggaaa ccgctttgcc gcagccctca gaacctcctt ggtcctctcg
     8941 atatccttca tggcgacatt cacacgcacg agcatggtga gcggcaacat gttatccggc
     9001 aagagcgcat ccgccgtttc gaatgcttgg cgggcggcgg aaaaattgcc cgccgccgct
     9061 tccgccgtcc cgagaccata aaatgctcgc gcgctcttcg gcgccacaca ggcggcttca
     9121 cggtaatact ttgccgcggc cgaataccgg ttctgttgaa atgcagactg agcctggcga
     9181 tccagctcct ggaacttcga cagcctctcc ttttcggtaa gctgcgacgg ctcgcaatcg
     9241 gcggctttca gggagcaaca caacaaaagg aagatgattc cggtcctcat gcgacataac
     9301 accgtaccgc cgcgcagaag ttcagctcat atccatcctt gcgcgccgga tcgcgggcga
     9361 gcggttgctg ccgcccggat tcgtccgtag cgcggctgag cagcacgtgt tcgccgggtt
     9421 tcgcgtccca caaatatctc caccgcaccc acgtaaacgg cttcgaaggc ccttcgagcc
     9481 tggccggact ccacgcgcgg cctccatcgt gactcacttc aaccgtgcga atacgcccgc
     9541 cgcccgtcca tgcgaagcct cgtataatgt gacgcgcggc tgggagtttc gcctcgtccg
     9601 caggccacgc aatcgcggac ttgaccgcga cttccgaaag acgcgtgacc ttccgctcgc
     9661 cgcccgccgc agcgactatg cggttgtaga ctgcattcat tccgctggct tcaaaatcgg
     9721 ccgcacgctc gttggcgccc agcacgatga tcctccgcag ccactttacc gaatccatcg
     9781 cgtaccaccc cgggaacagg gcgcgcgcag gaaatccatt ctttcgcgga aggaaccggt
     9841 cattcagctt gaacgcgacc atgctctcct cgcgcatgca tttcgcaacc ggcacaactt
     9901 ggcagtatgg cagcgcgggc gaattgtcca tgaggcttcc ggagtcggcc ccttcgagca
     9961 ccaccgtctc cgcgtcctga gccatccccg catcgcgcag gaggtttgcg agcggcacgc
    10021 cctcccacat cgcgttgctc accgccgacc cgccggccgg attgccggcg cactcaagca
    10081 cggcttcgag cttcctcgcc ggcgattcca ccaggtccgc gaaactcaac tccacgggct
    10141 ttgcgactcg cccgtcgatt ctcaacctcc acgtgcgaag agatacatcc ggctcgcgaa
    10201 aatgatcgcg cacgaaaaac aggtccggcg gagtgagcac gccctcgatg ctttggagcg
    10261 ggaacgagag gttccgcggc tcagacacat cgggcgcctg tgctcggaga aaacaggcgc
    10321 ccgtaaggaa cagaaagtcc ctgcgggtca tactcaggag gccctggctc ctagaaaatc
    10381 aattttagag ccatctggat aatgcgcggc gcccgcgcgc tggtgattcg cccgaagttg
    10441 ctgtccggca cagccgtgtc cggattgttc aactgcgtgt gattcaagac gttgaagaat
    10501 tccgcgcgat actgcagctt cagattctcg cgaatcgtga agaacttgtg cagcgagaaa
    10561 tcgatattga aggtccccgg accgataagg atatttcggc cggcgttccc gaacgtgccc
    10621 agcctggctt gcgtgaaggc cgacgtgtcg aaccatttca aaatccgcgc tcccagcggg
    10681 ccgctggtat aggtgggagg cttaacaacg tccgcaagat cgttgccgat tcccaccagg
    10741 ccgcggtcgt ccgctgacgt aatgttcagc gggaacccgc tttgccagtt ccagatgcca
    10801 gaggtttgcc acccgccgag aagagccttg gcgacgccct gtgacggcga cggcaattgc
    10861 cagaggtagt tcagcacaaa acggtgcggc acgttgaaat ctgcgacagc ccggtacgcc
    10921 cgaacattgt acggattgat tacgttcacg ccgcacaggt cggtgaggta agaaacctcg
    10981 tcgatggtct ttgcaaacgt gtagttcgcg ctaacagtga agccgtgcga gaaccgcttt
    11041 tccatcgaga cttgcagcga gttgtagatc gagtttccgc cggaaacgtc ttccaccacc
    11101 tcctggaaat acggattcgg ccggcgttgg ctttcgttgt cagccgtggc cgtagggctc
    11161 ggcaatggcg cgttcacgtc ggtgttgtag ccgaggtgcg tgcctttgga tgccgcatag
    11221 ccgaccctga ataacaagtc gttgtgtagc tgccgttcca cggtaaaatt ccaggccagg
    11281 gtgcggcccg gcttccaatc gggcgtatag gacacggcca agctcggcgg gatgatgaac
    11341 tgaacattcg acggcggtat gaagggcgcg aactgcgccg ggaacggatt cggatagttc
    11401 gcgtatggat tatcgaaagg cacattgaag agctggacct gcgggctcca cggtgcgctg
    11461 tccaccatgt tgttataagc ttcgacgaaa ggcggctggt agaacaggcc gaagcccgag
    11521 cgcaccaccg tctttcggtc gccgccgatg ttgtacgcaa aaccaacgcg cggcgcgaag
    11581 ttccaaggca caggttcgct gccgcccgtc gggcatcccg ggtcgccctc gtaaaggtat
    11641 ccgggcggcg cgttggggaa tcgcttcgag atcagccccg gacggtagca ctccgtccgg
    11701 cccagcgtgt cgccataagg atagaaagga tcccatcgca gccccagatt cagcacgagg
    11761 ttgggactga cccggtaatt atcctgaatg aaaagactgc caagcgtgcc gcgccgcgca
    11821 gcatattcgc cgcctccctg aataaaccgg tccacggccc ctagcatgaa gtcagaccga
    11881 ggatcgccgc tgtagctcgt gccgcggaat cgaaaatttc cattctggcg aaaagtgttg
    11941 atgagatcca cctgcatctt catcaggtcg ccgccgatcg cgatctcatg cgaacccttg
    12001 atccagtgca gactgtcggc gaaatggaaa ttctggcgat taaagtgacc gggatggccg
    12061 gtgctgatac tgaagtagcc cgacacggaa atgaccatct caggcggatt cgaggtggca
    12121 atcggaattc cgaggctggg aaagctgaac ggggcgccgc tcagaatgga tccgtttatc
    12181 cggttgaacg agaagataaa attgtttagc aaattcgggc gaatgttata cgtatcgctg
    12241 aacgatacgc tctgatcgcg gtcgctatag ccgttccttg ccttaatcag attcacgctg
    12301 cccacggtag gagcgtgcgt gtactcggag aaaaagtacc gcccgtagag ccggtggctg
    12361 gtcaggttgt aatccaagcg acccatcgtc tggttttcgt tatcgatctg cggaaggtcg
    12421 taggtgatga gggaatcggg gcgcgtcgaa tgcgggatgt agttcagcaa ctttactgcg
    12481 accggatcca gacggctggt cggaatgata ttacccggaa atggctgctt cgtgatggga
    12541 tccacgagtt gccgctgcac gcccgagaag tcgccctgcc gttgcgcatc ggacagcacc
    12601 gtggcggtgt tcgcaagcga tacgttgcgc agccgcgtgc cctgataggt tccgaagaaa
    12661 aagaggtggt cctttttgat tgggccgccg agcgccccgc cgaattggtt gcgcttcagc
    12721 agatcgtgcg aagcggcgaa gaagttgcgc gcgttcaggt cgccgttgcg gaggaactcg
    12781 aaggcgctgc catgaaacga gttcgtgccg gacttcgtga cgacgttcac aatcgcgccc
    12841 gcgccgcggc cgtattcggc gctgtagcta ttggtctgta cactgaactc ctcgacggca
    12901 tcgggattcg ggaacggatt gttgacgttg gtgtagtggt cctgattgct gccgccgtcc
    12961 aggttgtaat taatgtcatc ggcccgcgcc ccgttcgagc taatggactg ggcgccggga
    13021 tacgttgcgg tctgctgcgt atcgccgctg ccccgcgcgt tcccggcagt gaggtccgct
    13081 gttccgggag ccagcaggac cagggctgcc gcattacggc cgttcagcgg caactcggaa
    13141 acgctctggg tcgtgaccac cttgctcagc gtgctgcttc gcgtttccac cagctctccg
    13201 ttggccacga ccgacacagt ttcagtcgtc gcgccgattt ccagcttgac ggcaaccgtt
    13261 gtgttctgat cggcgcgcac gtcgataccc ttctgctcaa agcgccggaa tccgggagct
    13321 tccaccgcaa tggaatatat tccgacgggc agcagcggaa acacgtaacc gccatcggcc
    13381 gtgctcgtcg tttcgcggct caagccggta ttgaggtttg tcgcggtgat ccttgcgccg
    13441 acgacggcgg cgccgctagg atcggtgatc gtgccggcga tcgtgcccgt cggctccgcg
    13501 ccataggcgg acaatgccag aaagagacaa ccgactgaaa acaacaggcc gcgcttcgcc
    13561 atgatcccct cctggagcgt tgagccagtg agagtcgctt ttgggagagt ttattggcaa
    13621 acatgtgcga tgtcaagggg aagaagagtc ctcgccgccg aaggcctgaa accgctggcg
    13681 ggaaggggtt gtgggaaatg ccgggggaat ctcgccaacc cgcgagattt ccacattgcc
    13741 acagcccggc ttcgccccgg atggaaaagt ggaaaaccaa aaacgggttt ccactttccc
    13801 acacacggct tgccacgacg ctctttgttt cctccctcaa aaccgaaaac gcaaagaagg
    13861 aagtcggccg ctgcgcggcc tccccgtcct ggtttttcag gatcactctg cattggaaac
    13921 ggatccctgt ttcaggatca ttcttggact ggaaaatgct cctcggctgc ggtttgccgc
    13981 cgcaatgcgg agtcgagcac ggcgcgcagg gtgcggagca gatcttccgg acggtacggt
    14041 ttctgaatga aggctctggc tccgagcgcg gccagatctt cattgccgtc ggccgaatag
    14101 ccgctggcca atacgaccgg gagttcgggc gcaacttcgc gaagcgccgc cagtgtatca
    14161 cggcccgatt tgcggggcat cgtcatgtcc agcagcacga tgtcgatgcg gccgggcggc
    14221 cccggcaggt cttgccgcac aacttccagc gcttcctcgc cgtcttgcgc ctgcaaaacg
    14281 cgatatcccc ggcgctccag aatgtcagtg gcgagggttc ggatcatctc ctcgtcgtcc
    14341 acaagcagga ctgtttccga gcccttcgtg tccgaaggcg ttacgcgggg cgcatcttcc
    14401 tcagccgcct tgggcgaacg cggcagaaac accgaaaaga cggagccgca gcggggctgg
    14461 ctctccacgg tgatccagcc gccatgctgc ttcacgatgc cgtacaccat cgaaagcccc
    14521 aagccggtgc cttttcccac ctgttttgtg gtgaagaaag gctcgaagat tcgcgacagc
    14581 gtggcctgat ccatgcccgc tccggtatcc gccacgcgca agcagacaaa ctcgccgggc
    14641 cgcgcttcgg gccgcgaccg gcaataatcg gcgccgatca ccagattggc ggtcgaaacg
    14701 gagaggcagc cgccgtcctc catggcatcg cgggcattca ggcagagatt cagaaggatc
    14761 tgattgatct ggccccggtc ggcctcgacc agccacaaat ccggctgctc gctggtttcg
    14821 atgataatac gcggatccac tgtgcggcgc aacagatcga cgctctcgcg gacgctggcg
    14881 tttagatcga tcgggcgcgg gcggatgggg ctcttgcggc tgaagccgag caattgctgc
    14941 accagctcag cagcccgctc ggctgctttc tcggcgtctt ggagcccccg cccggccggg
    15001 tgcgccgcgc ccaagcgcat gagcgccaaa gaaatattgc cgacaatgac agtcaagaga
    15061 ttcttgaaat catgcgcaat gccgccggcc agcacgccga tggcttccat tttttgcgac
    15121 tggcgcaact gggcctccag gttcttgcgt tcggtgacgt cctggaccat cgaagccacc
    15181 gcgactacag cgccggtctc atcgatcagg gaggtgttga accattcgca ttcgatcacg
    15241 ccccggtctt tggtcaagtt ctgggtcacg cggtgatcgc ttctttgcga gagctgtagt
    15301 tccttccagg cggctacaat ttccagccgg ctttccggag cgaccagaag ctcgaggccg
    15361 ttgcggccga aaacttccgg cgcgctccat ccgaaaatgc gggacgcact gctattccat
    15421 tcgagaatgc ggaagtcgag atcaaagacg atacagccca ggggcatatg cgagacaaga
    15481 aactcgagcc gctgtaccag ctctcgctgg cgctcctccg ctttgcggcg ctcggtgatg
    15541 tcgcgaatta cggattgata tccgagcacc atgccccgca gacaaacggg gaccacggag
    15601 ctctccagat acaacgtcgt tccgtctgag cgcaggcccc gaaactcgaa acgggcgcct
    15661 ccctccgaat cggcgaacaa gggctcatgc caatcatggc ggagccggcg gtcctcggga
    15721 tgaatgcaag cgtcgagccg aagaccgcca atttggtcgc gccggaggcc gaacatgtgg
    15781 aggaagcgat cgttggcgaa gaccagacgc ccgcgcagat cctctgttat gaccgcgtcg
    15841 tgaatattgt cgagcagcag ccgcagtctt gccgattctt gctgcaaatc aagcgcgaga
    15901 tcgcggttct ccatttgcac aagaaattgg cgcgccagca ccagaacaac atagaccccc
    15961 aaagaaacga gcagggtctc acgccggagc tcaaccatcg gcgcgaacgc ccccggacgg
    16021 cttccgaaga atttttcgat caagggaatg cccccgattc cgagcagcgt tagaaaaggt
    16081 atggaaagct cgcgccagac cgaaatgatg ggaggctcgg cggtgctgcc ttccttgggc
    16141 gaagcggccg gcgccgcggt tggatggcgg gcggcggagg cgaggaggaa aaaagcggct
    16201 gtctggagta catcggctgc ggcaggagtc gcaaatgtgc tcggggcaag agatccatag
    16261 gaaagggcta agatagtccg gctggcggat tggcacgccg cagccagcgc caagtcgcga
    16321 taggtgcgct tccatggccc gggctccctt ctatggcggg atgcggtaag actgccggcg
    16381 caaagaaggg ccaacgtcag cgggaaaaac gccatgccga aagaaatcga ccgggccgag
    16441 gcgagccggg ccgcggcagc gatggaaaac tcccaatcaa gcgttagaaa gccgagcagg
    16501 agcgtatcca ccactacagc cggccggatc gaacgcaggg gaactcgata gggcttcaga
    16561 agggcggcga ggccgaggcc gatgaaaacg aagtagccgc aggtggtcca gagggcgaaa
    16621 tagatgaccg gcacagattc caacgcggcc cggtacggtt ctgcggcgcc gcgccgcatc
    16681 caggcaagac tcgcgaccgc tccaacgaag gccagccacg ctccggcgga caagaacatc
    16741 caaattaccc gcagccgccc ggcggagcgt cgcgaggcgc gagagcacat gaatccggcg
    16801 gcggctgagg ccgcgcagga agctagggcc ggcagagccg caggaaggcc ggcgcgagga
    16861 accgggcccc agacgacctg gcttgggaga aggaggaaag ggaggtgggg acctaagagg
    16921 atcgtccatc gcgccatttt tgtggtttat cggcggattg tggcaaatac tcaacacggg
    16981 cggtccaccg gcacggccgg gccaagcagg ctggcggttt gcggtttttc ggctacaatc
    17041 gagagtccat gctgctgacc ggaccgcctg gcagcggaaa aacatctcgg gtgctcgccg
    17101 aattgatatc ggctctggcc cgaggccaag acccgcgtcc cattctaatc gttccgactg
    17161 ccagcatggc ggaacacacg cagcaccgga tcgcgcggca aggcatcctc gtcccgccaa
    17221 aagcaatact gccgctccaa tcctatatcg agcagttgac gccagcgtcc agagaagctt
    17281 ccgcggcggc cgaacaagtg ctgctcgagg ccgcgctgcg cctggtagat acgagcgagt
    17341 ttggaccagc cgcagaatat cctggattcc agcggatttt gctcgatacc atccaggaat
    17401 tctggtccgc gggagccgac ccgggggcat ttttggaaaa agctgagtat aaaccgtatg
    17461 gcgcgccgtt cctgcgcatc atgagggaat tcgagaatca gttgcatgcg gcgggatacg
    17521 ttaccagggc cgagcgtttc cggatggcag cggctgaact cgggcggcag cggttgaaaa
    17581 gcgttttcct ggatgggttc ttccacttca ccgccatgga acaggagctg gtgaaagcgc
    17641 tggccgggac agcggagaaa atcctgatca cgttgccgga gaccggcgcc gaacaagccc
    17701 ggcaggcgct tcttgcaatg ggcctgccgg aagagcggct gacggagctg aggcggttgt
    17761 ccaaaccggt tctggtgcgg gctttctccc cagaaggcga aatagaagac gtcgccgggc
    17821 ggattttaac cgatcaaaag aaaaccgggc ggccttttta cgaatatggc gtgatcttga
    17881 gggacccgga ggcttatgcc ccgattgtgg cggtggtctt cgagaggttc gggattccct
    17941 ttcggctcaa gcagccacag cctctgactt cgcacagcgc tatccggtat ctgacgggcc
    18001 tgttgcatct ggcggcgggc ggcttcgacg gggaagcggc gctggcggtc atgaagctgg
    18061 cgccatcggg aatcgccttc agccgggaga tggatgggta tgagttcgag gttcgaaaaa
    18121 gcttgccggg gcgaggcctg ccttttcttc tcgccctcgc ggaacgccgg ccgctggttc
    18181 tcaaatggat tggaaagctg gagcagcttg ccgactggaa tcgggagcgc ctcgcgccgg
    18241 ccgactgggc gagccggtgc gagggcctgg cgcggacttg gctgcgggcg ccggtggtaa
    18301 aagacggcct cggcaactcc cccgtgttag agctaagagc ttgggcatcc gcggctgacg
    18361 cctggagggc ctcggcgcgg gaggcgtccg aattgctggc cacatgcgga acgaaaacgg
    18421 ccgatctgcc gcaatatctt gaaatgctgt cgaaggtatt gcgtgcggcg ccgcttcgag
    18481 tggccgaccg ccgccgcaac gtagtgcaag tgttgagcgt atacgaggcg cggcagtggg
    18541 agcttgccgt agtcttcgtt tgcggcctga tcgagaaaca atttccgcgt tatcatccgc
    18601 aaaatctgtt tttccccgac gttcagcgaa gggccttgag cgcgcagggc ctcgtgcttc
    18661 gaacgagcga cgaccgcgac cgggaagagc gatttctgtt cgacttggca ttgacgcggg
    18721 ccaccgaaca agtctatgcc agctacccgg cgcacgacga caagggagcc gagacagttc
    18781 ggtccttttt tcttgagccc tggaaggaac aggaagtacc ggcacaggaa gctcgatttg
    18841 ccggaggggc gcaggttccc tccggcccgg tggtttgggc tcagaaagcc gtcttaaggg
    18901 atcccggcct gctacaagtg ctcggggaac agcccagcga gttcagccct tccgggctgc
    18961 aaaagtatat ccagtgcccc tttcagtttt ttgctgcgcg catcctgcgc ctgcaagagc
    19021 cgccgcccaa acctgacgaa cgcctggatg cgatgctcca gggaaagatc attcaccaga
    19081 cgatcgccca gtggatgaac agccgcgaca ggccaatccg ggaagtgggc gaggaggttt
    19141 tacgccagac ctgcgatgag gaacatatac gtctcaactt ccgcgccgag gcgatgaaac
    19201 tgcaacttct agccgatttg gaaagatttg ccgagagcga ggcggcggcg agcgcgccgg
    19261 cgggatacca agccggcgag ccggaaaaat ctttccgcta caccctggcg gccggcgcgg
    19321 gagcgcctgt ttgcatcacg ggacgcatcg accgtcatga actttccagc tccgccgcgg
    19381 ccatcgtgac cgattacaaa tattcacggg cggaccggat aacggagttg gtcaagcagc
    19441 atgaaaaagg ggaagcgctg caaggatggc tctatctgct cggtctgaaa aacgagttgg
    19501 gagttcagcc ggccggctta gtcttttacg gattgcgcaa ggagatttgc cgcaagggat
    19561 ggtatgtacc agaactggcg ccggcgggcg acggactgga ggaagccagc gccgcgcaat
    19621 ttcagcagat gctcggacgc gccgaggcac gcgcgctgga aatcgtgcag gatctccgaa
    19681 aaggcagaat cgaagtggcg cctagggacc gccggctgtg ccgcgaccag tgtccttact
    19741 tggacgtgtg ccgggtggat ttgtaggcaa tatctggtgc ttcctgaccc gcgccgccgc
    19801 ccatcccggc ccgctgcgaa cgaccgtgcc caacagatag agatttacat ccacaccgtc
    19861 cggcgaagtt tgggtcatgt gcacgaccat ctccagtttg cgccccacgg caagctgctg
    19921 gtcggtgtga aacaggacgc cgctgctgct gatattgatg gtgcggccct gatgaggggt
    19981 ggcgcctttc gcgtcagcca ctctaaattc cacgagacgc tcgatgtcaa agcggattgg
    20041 acggtcgatc attgggctat ccgatgatcg cgtgtgtatc gaagcaacga caataggcca
    20101 gcgggtctat tttccatttg gtacgatggt acccgaaatt tgggaggtcc gttggctttc
    20161 gttcctgtac ttcattcagt cagctacgca ggggcttggc ccggtcaggc gtgcctgagc
    20221 ctggagcaat tcctggcgaa ggcggcggaa ctgggttttc cagccgtcat gctgatggcc
    20281 aagaggcctc acctgtcggt gctcgactac gaccggaaga gctgcgagcg gctgcgccgg
    20341 ctgatggacg acaagggaat tgaatgccgg gtgatcgctg gctacaacaa tttcagcgcg
    20401 gatatcgagc atggggacgt gccaaaccgg gaaatacaga ttcaacacgt aacggagctg
    20461 gcccgcatgg cgagcgacct cggggccggc attgtgcgga tcttcaccag ctacgagagc
    20521 ccggcggcca gctactccac gctgtggcaa acgacggtag attcgattcg ggaatgcgcg
    20581 cgacgagcgg ctaagtacca agtgacaatc ggcgtccaga atcatcatga tctggcggcg
    20641 cattatgaaa gccagtttga cctgatcggc gccgtggggg aacccaactg ccgagctatt
    20701 tttgacgcct gggcgccggc cctccaaggc acggatcttg gaaaggcggc gcagattatg
    20761 gcgccgatca cgtgccacac aactgcggcc gattacaaac gcagaccacg atttcgatac
    20821 catccaaaac tggtcaacta cgaaaaacaa atcgactggg tgcaggcggc gcccatggga
    20881 gagggattta tcgactacac cgggttcttg cgggctttgc aaaggggggg ttacgccggc
    20941 ggtgtagcct acgaaatgtg ctcgcccttg gccggcggcg ggacaatcga aaacctggac
    21001 tgttatgccc ggcggttcct cgaattttta aacagcgttg aattcgaaac aaccaagtag
    21061 aggactgccg gccgcgccgg ccggtgtggg gagtgcgggg ttgaccagaa acgtgatgca
    21121 cgggtcgcgt gggaacacct gcccgggtcc cggccagccg cgctattcga ccggtatttt
    21181 ggttcccctt tccgattggc gccgccccag ggcttcaatc tgagcatcca atacggcccg
    21241 aacacccttt aacacttcaa tccctgcgtt gcggaagtgc tcaagagcct ggctgctccc
    21301 catggctccg gtcattagcg cgagcaagcg cggagcaacg gtccaacaat aatatggggg
    21361 cggtggcgcc tgttgccgga ccccttcgcg gcgcggatta ttgcgccttc gattttctct
    21421 tgtcatttgc ggccctccac tagttcaacg gtgaggatcc cctcttccag tttcgccttg
    21481 cgcggttcca aagccgccat tgtcgttggc aaaccgacat gccgccgcac ggcgcccact
    21541 tcgatgacaa gttcatcgcc ctttttgaat acgctgacct ctcccggagc cgcgaaggcc
    21601 agatggagcc gaatgcggta gcccgagcca ttcttttcaa aatggtaggg ggcttcctgc
    21661 cgggaacggg cggccgggtc gatgtcgggc tcctgataga gcactcgcgc aagctccgcc
    21721 agccgggccg agccgaccac ttcgtgcgcg aatagtggaa cgcggctgac aggaaccgga
    21781 gcgaaatact cttcgatctg ctcgattgtt ttctgctgcg agcgctgcca atcgcggaaa
    21841 tagcgatcct ggacagccgc gggaagcacg cgattcacga taatccggtc cacggtcatg
    21901 ccgtgcaacg cgaaatagac aaaggcgcgc tgggtctccc gcaacaccat tttctcggga
    21961 tttgtaacca ggcgcacagt caccacgcgc gaatcttcca gcaattcgcc gatgccttcg
    22021 agccgctcga agagtttctg aatattgctg aaatagctgt cggaaggcag cttcacgggc
    22081 gagacgcggt tagcgaccgg acgcacggcc ttcatgaggg tgcgctcgag atggaagatg
    22141 tttttcatgt accactcgag ggtcgtgggc atgctcacga agcggaggga ttcggcggtg
    22201 ggagcacaat cgacaaccac tacgtcgtac tcttttctgc gacggtattg gttcacgtac
    22261 atcagcgagc tgacttcttc catgccgggg aatatggcca gctcctcggc ctccacttgg
    22321 gtaatgcccg tggcctgcaa gagggagacg atgtaagacc agatttcctg ccaatggcgt
    22381 ttgatttcct gctgaatgtt tacttcctgc acggcgaggc gtttgttaat ggaatagggc
    22441 aggccggtag cagcggaaaa caggctctga tcgaggtcga aggcgtcggc gaggctgtgc
    22501 gccgggtcca cgctcattac gagtgtgcgg tagccccggc gggccagttc cagcccggtc
    22561 gcggccgcga tgctggtctt tccgacgccg ccctt
//
LOCUS       DASREK010000266        20987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2276288, whole genome shotgun sequence.
ACCESSION   DASREK010000266 DASREK010000000
VERSION     DASREK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 20987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20987
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276288"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFA54_14410"
     CDS             complement(<1..717)
                     /locus_tag="VFA54_14410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZS52056.1"
                     /translation="MKTHFIRDLQPNEVVTSFFLVHHKEVRLKKTGEPFLCLTLADRT
                     GTLDAKMWDGVPEVMETFDRDDFVKVKGLVQIFRNKPQMTVHKLRRLADSEVDYADYF
                     PHTEKNIDEMWLQLRAAVSAIQNVPLRALLDAFLDDEDIAQRFRRAPAAKSLHHARIG
                     GLLEHVVSVLGLCRLCSEHYGFIDLDLLITGAVLHDLGKIFELQYDRSFGYTTEGQLL
                     GHMAIVMDMLHAKAALVPGFP"
     gene            complement(740..1948)
                     /locus_tag="VFA54_14415"
     CDS             complement(740..1948)
                     /locus_tag="VFA54_14415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZS52057.1"
                     /translation="MKKSCFLVFGLIWAALLGAAASVQVLEEIIAKINGEIVTRSELE
                     RSNAEARAELARQKLTTQQFENAVAEHEKNALRDLIDQSLLVQRAKELNVNVDSAVVK
                     RLDDLRRQYNIPTMEEFEKFVSEKGGMPYEDLKDQVRNQLLTERVIQQEVGSHIVVTK
                     EEVAKYYEEHKQDFVRPEQVRLRQILISTEGKDPKDIPALEKKANEILARLRKGERFA
                     EVASKYSDDQESARAGGDIGFWKRGLLDKEIEDIVFNAKRGYITEVLKRRNGFLILQV
                     EARNQAGLASLEDVEQEIQERLFMPKMQPALRAYLTKLREAAFIEIRPGYVDTGAAPG
                     RDTTWKDPENFKPAVTTKEEVTKKKKKLFWVVPRGNSSTTPAPAGGSAPPPSTPGVSS
                     SPANGGRQPQ"
     gene            complement(2197..5577)
                     /gene="mfd"
                     /locus_tag="VFA54_14420"
     CDS             complement(2197..5577)
                     /gene="mfd"
                     /locus_tag="VFA54_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZS52058.1"
                     /translation="MTLPFVRDLFLGLERHPSFQELLKEIRRKAARSLQLSGLVPVAR
                     ALFSVLLHQALDRTVLVLVPSDKEADLLAEPIETFYRLLFSDRQAGPVTLPAHDVLPY
                     DGLAPHPEISERRAIGLWRLSSERPPLVLTTIAAALRKLHAPEYYHGLAFRLARGEEM
                     PLDLVIAELDRIGYQRHEPVEMVGQYSVRGGILDVYSPESARPVRIEFFGDQIESLRR
                     FDIETQRSVLSVDEALLLPLAEETSTPAGPLTGSLLDLAPDCLLVFDGLSEVLAAAEK
                     LWQQLEAAYPKSEQILDPPASYYWAVREWQEQAARFPQVSVEELGLDVSPGSFHIATG
                     PATRFQGNIPHCMRELESEIQQGWRAVFFGATAGEVERLADIFNEYGVSFQLGLKDPC
                     SAAARYLEEKAYLAGEFASVTLVQGAVRRGVRFPDSQLAVHGSDDLFEVSEFVAKPPS
                     GKSRLSTFLSDFQDLKPGDYVVHVEHGIGRYTGMQQIGDNGQREEFMALEYAENAKLY
                     VPLSRLDLVQKYRAPEGVRPQLDRLGGATWTRTKTRIKARMRDMAEELLRLYAQRKLA
                     TGFRFSPDSNWQREFEDAFEFTETEDQLAAIRDIKRDMESESPMDRLVCGDVGFGKTE
                     VAMRAAFKALGDGKQVAVLTPTTVLAFQHFETFKQRFSRFPVKIEMLSRFRSTKEIEQ
                     ILADLADGKIDILIGTHRLLSADVVFRDLGLLIIDEEQRFGVRHKERLKQMKKGIDVL
                     TLTATPIPRTLHMSLVGLRDMSVIETAPKDRLAIQTVVAPFNEKLLRTAIEQELERGG
                     QVYFLHNRVESIWSIASLLQTLVPRARIGVGHGQMSERELEKIMLKFMHHEYDILVST
                     TIIENGLDIPLANTIIINRADRFGLSDLYQLRGRVGRSNRRAYAYLLVPEDAELTPLA
                     KKRLAALKEFSELGSGFKIAALDLELRGSGNLLGGEQHGHIAAVGFEMYCQMLENTVR
                     ELRGEQVMAAGPVSINLHLDVRIPPEYIPEENQRLRTYKRIAGLQSEEEREKIRQELV
                     DRYGPLPEGVANLLEYAALKMIAERIRVDAIDRRQDRISIKFNEAASVDPQKLMAFAA
                     GAPGAEFTPAGVLRFTMNGGGAAHTIQEITELLKQLQA"
     gene            complement(5574..6047)
                     /locus_tag="VFA54_14425"
     CDS             complement(5574..6047)
                     /locus_tag="VFA54_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZS52059.1"
                     /translation="MGRVLDRDELLAERRRWKERSQKTVLTNGCYDLLHPGHIRLLER
                     ARSLGDRLIVAINSDSSVRGLKGPLRPIIGQDERAELLASLEAVDAVTIFDEETPCEL
                     LSLLLPDVLVKGADWSHWIAGREIVEAAGGQVLALPLEPDFSTTGIVETVLEQQR"
     gene            6201..7460
                     /locus_tag="VFA54_14430"
     CDS             6201..7460
                     /locus_tag="VFA54_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS52060.1"
                     /translation="MVATLAAAPQTFRPSNPVTLQDAGLNQNLLIDLMLKLTLLEGET
                     TLHRLSSQMKVSMTIMDQLFRYFRREQLCEVKGMVGNDYALSLSAAGRKIAQERYSIN
                     QYIGPAPVKLNDYCEAVKAQAAHHPVSRSMLSEVFFDLVLTEPVLDQIGPAIISGTTI
                     FLYGSTGNGKTSIAERLVRIYKDYVYIPYAIEVEGQIINIYDSVVHQALETQEDDTDP
                     RWLLCRRPCIAVGGELVPEMLELRFDDNLGFYSAPLQMKANNGLFIIDDFGRQMINPR
                     DLLNRWIVPLDRRVDFLSLRSGVKFPIPFETLVVFSTNLDPRQLADEAFLRRIQNKIK
                     VDAITPEIFDKILERVCEEYQIPFDPEMAQYLREKCNPDGKGVLRACYPRDMVRILQS
                     IADYENRAPQLTKKDLDRALNLYFAKT"
     gene            7551..8516
                     /gene="add"
                     /locus_tag="VFA54_14435"
     CDS             7551..8516
                     /gene="add"
                     /locus_tag="VFA54_14435"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZS52061.1"
                     /translation="MNLDFPKAEIHLHLEGTIEPQTLCELAARRHVALTEEEVAGRYR
                     YADLLGFLQAFKWVTQYLQTPEDYQIAALRMFEKLHRQNVVYAEVYFSAGICLWKGME
                     VDPIFDALEEARRRAEADWGIRARWIFDAVRQFGIEAAERVFRLAARWQHRHVVGVGI
                     GGDEKRGAPAMFQTLFEAARSAGLRLLAHAGENTGPEFIWESIRKLGAQRIGHGVSAI
                     CDPALVQYLRDTQIPIEVCVTSNYATGVVAPGAEHPVRRMFDAGLLVVINSDDPAMFG
                     TTLNDEYRRLVERHGFTEAEIAVLAQNSFQAAFLTEQERQEFLKA"
     gene            complement(8610..9512)
                     /locus_tag="VFA54_14440"
     CDS             complement(8610..9512)
                     /locus_tag="VFA54_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52062.1"
                     /translation="MGALFTLFAAIALGAADQTATPPADPGADSARDIIRRSTVLDER
                     NFRAAREYTFTERVVERKLDAAGRERSVSSKTYEITFLFGLPYRRLVARDGRPLPAEE
                     AREEEKKLGRAFSASQRQSQTERQRRASEYEKRRERTWAFLREVPDAFDFRLLGEEML
                     HGRPAYVIEAAPKPGYRPRNSEARLFTKLRGKLWIDKAELQWVKGEAETTDTIAFGVF
                     LARLAPGSRLEFEQAKVNDEVWLPHRISYAGSGRVALVRTVHAGGEILYGNYRKFQSD
                     FEITIPAGHSALGAPGASAPSSNQ"
     gene            9927..10916
                     /locus_tag="VFA54_14445"
     CDS             9927..10916
                     /locus_tag="VFA54_14445"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZS52063.1"
                     /translation="MQRATPSEEKKSEIRKFKTTKTIIWRALLSWQFELMDPPYGAVS
                     EGPVWTGSKLLFTHIQESRILQYDPATGLCAVFRDGTNCANGLALDQQGRLYGCEGGA
                     TADARRVVRYEAGGGVTVLADSFEGKRLNIPNDLVLDPQGRVWFTDPFYEGAAGPWSF
                     DRANKELNHDSVYRLDPQPDGSYSIHRVTFDTTRPNGLLFSLDYQTLYVAQSGRLPEE
                     KRQLRAYPVRSDGRLGAAEVLHDFGEHRGIDGMRLDVEGNIIATAGWELGGPGPSIYV
                     FSASGEVLERHAVPAKRPTNCCFGGGELSDLYVTTTEGHLFRAHTGRCGRRYP"
     gene            11135..11995
                     /locus_tag="VFA54_14450"
     CDS             11135..11995
                     /locus_tag="VFA54_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52064.1"
                     /translation="MSATAAVTATDRNLEDQIFQLQKMDLDSRVKEIVEEAAQLSMQG
                     RHLEAAALVEKAEAMRAAIAAETPSAGLPPAETQPPAEGEQVVARLVGDVANGLAKVL
                     VSALQDLEQHIAGETRKLATSFNQQLDRLQTTVESLLPLGERIEQLAGAIAEQKAVGL
                     AAHEKCSQLAAETALLREADGRYENALGSLRAEMQERTASVSERLEALTGRLGLQQEE
                     ISAVKSSVSDLSPRVATLVERLDRQAEAIRCLYETESHRGAAMDELVNAMGRLRALHL
                     PPPEQPATQL"
     gene            12253..12576
                     /locus_tag="VFA54_14455"
     CDS             12253..12576
                     /locus_tag="VFA54_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52065.1"
                     /translation="MLIFPAYPQVTEIYTSTRSLIGRVQADLKRAEDFVPPSQKERER
                     YENAQRHLSELDRELSKGKFDKEKLDSAIDDVKNVVENNTLTPDSRDALTADLRSLRE
                     LRAKH"
     gene            12729..14762
                     /locus_tag="VFA54_14460"
     CDS             12729..14762
                     /locus_tag="VFA54_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52066.1"
                     /translation="MRRYLHLLWLPVLGLGACRAASVPVDLSAVRPGPVSVEAKEKSL
                     LVRWPDESSRLWRAEFSLDPSEPLITEIAAGDKKVITGAVPLYWCESGKRRGGWDNFF
                     DAPATHPEGMRRWVGRFKLTSVKARTIGDRVEVAFNGLRLGVFRGAVAYIFYPGSRLI
                     QQEAVVSTREPDTAYFYDAGLQMLIPSERTPGLFSMDARATYYDTSGELRTEASDYER
                     KAVAARYRTIAAATPAGSVAVFPPPHQYFSARDVTTNLRYVWHAAWGGKVAIGIRQIA
                     DDNSPYNPGINAPPGAQQRMSLFLLVTEGDAATALRDSLRYTNRDRFPALEGYQTLSA
                     HWHWAYTMQALQHGPDWIPPFKPVLKAMGVEAAIDMDFHGDGHPRDLSETRLKELDAF
                     FRACRAQSDSEFLLIPSEEANVYLGGHWALIFPHPVYWFMSRPEGTEFLSQDARYGPV
                     YHIGSAADMLEMIRREGGILYQTHPRTKGSTGYPDKILKTDYFRDPRYVGAGWKAMPS
                     DLSSFRLGRRAFGVLDDLNNWGFRKRLLGEVDVFQIDSTHELYGHMNVNYVRLARLPS
                     YDNYGQILDAVAKGDYFVTTGEILLPEVSISGQGGEILVRAAARWTFPLQFAEVVWGD
                     GSETTHKTFPLETTREFGSGTFEWKVDAPGWKWARLAVWDAAGNGAFVNPAWK"
     gene            14924..15793
                     /locus_tag="VFA54_14465"
     CDS             14924..15793
                     /locus_tag="VFA54_14465"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZS52067.1"
                     /translation="MSTQVINISNRSITAHQTMNWPTIIALVFFHAGAVAALFVFNWR
                     AFLAAGFLYWLAIGVGISLGYHRLHTHRSYKVPLFLEYFFALCGSLTLQGGPISWVAT
                     HRIHHQKSDQPGDPHSPRDGGWWAHVGWILFGEAVHNNTRAMSKYAPDLARHRFYRWL
                     NNYHWVPMVVLGFILLAAGGLPMMLWGICARVVVGLHTTWLVNSATHMWGRRRFATRD
                     DSRNSWWVALLTFGEGWHNNHHAHPTSARHGLAWYEFDPSWIEIKVLELLGIARSVRV
                     ARLDSVLPKHNSA"
     gene            15887..16777
                     /locus_tag="VFA54_14470"
     CDS             15887..16777
                     /locus_tag="VFA54_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZS52068.1"
                     /translation="MVVAGRQKSIAFFIALGAGLVVVILLLYIGWVLLNWRHGILLFL
                     GALLLAMIIAGVVVNTTFLVREIRRNAQHDAFINAVTHELKTPVASIRLYLQTLQTRE
                     VDEGKRREFYRTMLEDSERLLATIEQVLRTGRIGASGRKLHRSPIDLGGVIEECVSRA
                     RTLYRLSAESLQYHPGPAVTILGDVDEVQAAVLNLIDNAVKYSGNNVKVIVETAKNGN
                     YVSVRVKDQGPGIPVSELKQIFKRFYRAPGSLATRVKGTGLGLYIVRSVAKRHGGSAW
                     AESEGPGRGSTFVLQLPILK"
     gene            16774..17511
                     /locus_tag="VFA54_14475"
     CDS             16774..17511
                     /locus_tag="VFA54_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS52069.1"
                     /translation="MIHILVVEDEQHLAEGLRFNLEAENYEVTVVDTGEAALEALKEG
                     ESQHFDVVVLDVMLPGKDGFAVISELRQSGQFIPTLMLTARGHPDDVLKGFAAGADDY
                     LTKPFELPILIARIRGLLRRRDWLLGSVNSANTPAARGDTFKFGDKSVNFDLLELHVR
                     DQVFPLTLMEMNVLRYLIQHEGRPVSRKAMLEEVWGLHEDTDTRAIDNFIVRLRRYIE
                     EDPTRPRHLLTVRGVGYRFVAAPVKSG"
     gene            17748..18692
                     /locus_tag="VFA54_14480"
     CDS             17748..18692
                     /locus_tag="VFA54_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS52070.1"
                     /translation="MKLPAIRIPAFTALLAVWSAPGVAQAPPRPRITGLAHVALYTHD
                     IEKARWFYKDFLGFSEPFLLNNQDGSLALTFLKINDRQYIELFPEIKPQTDRLNHISF
                     EVDDAEAMRVYLGSRGIPVPEKTPKGRIGNSNFNVKDPDGHTVEIVQYESDGWSMRDK
                     GKYMNEARVSRHMMHAGILVGALEPAMRFYRDILGFREIWRGSSNGKQLSWVNMQVPD
                     GTDYIEFMLYNELPAPASRGVQHHIALETPDMALAAALLEATPARKDYARPMEIRIGV
                     NRKRQMNLYDPDGTRIELMEPKTIDGAPAPPSTAPPPR"
     gene            complement(18952..19028)
                     /locus_tag="VFA54_14485"
     tRNA            complement(18952..19028)
                     /locus_tag="VFA54_14485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18992..18994),aa:Arg,seq:acg)
     gene            19210..20595
                     /locus_tag="VFA54_14490"
     CDS             19210..20595
                     /locus_tag="VFA54_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS52071.1"
                     /translation="MSAPSRRHVLIVDDEESQRQALATLVASWGYTPQTAEDGQDALD
                     KLAAFPAQVIVTDLSMPRLDGFALLRRLREQGAAPPTIVLTAYGSIENAVATVHDLGA
                     YWFLEKPIQPAALQVLLERALEKSNLSQEKERLQRRLAYSGELDEMVGRSRLMQHVFS
                     MIQQVAPTSACVLITGESGTGKEMVARAIHQLSSRREGPFIAINCAALPETLMESELF
                     GHEKGAFTGAAERRAGCFELAQGGTLLLDEIGEMQPLTQAKLLRVLEDGRVRRLGAKS
                     ELQVDVRVIAATNKVPEVAVARGELRQDLYYRLNVFHIQLPPLRDRREDIPLIAEALL
                     RDLNRKHSCKVTGLDAAVLERLERHDWPGNVRELRNVLERAVIVAGEGVITMNHLPAG
                     FMTPSQSPSQPTSDGDSVVLRVGATVADAEKGLILKTLEHTSNNKTRAAEILGISLKT
                     LHNKLKEYGAA"
     gene            20601..>20987
                     /locus_tag="VFA54_14495"
     CDS             20601..>20987
                     /locus_tag="VFA54_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52072.1"
                     /translation="MSFKRRLSVSIVALVLALTAALSIVYLQRLVRMQLDSALQQARM
                     VAQQVESATLQTISEPPPSSTLEESLSFWRNAVRVDDQLKEVLLRSISSFTTIAEIAV
                     TDDQGRILAGSVERERWVVRPAFSELL"
BASE COUNT         4140 a         6318 c         6327 g         4202 t
ORIGIN      
        1 cggaaacccg ggaaccaggg cggccttcgc atgaagcata tccatcacga tcgccatgtg
       61 gccgagcaac tggccttcgg ttgtgtagcc gaaagaccgg tcgtattgca gctcaaagat
      121 ttttcccaga tcgtgaagca ccgcccccgt aatcaaaaga tccagatcga tgaagccata
      181 atgctcggag cagagccggc acaggccaag cacggaaacc acatgctcga gcaggccgcc
      241 gattctcgcg tgatgcagcg acttggcggc cggagcccgg cgaaaccgct gcgcgatatc
      301 ttcatcgtcg aggaaggcgt ccagcaaagc gcgcagagga acgttctgta tggcggagac
      361 agccgcccgc aactggagcc acatctcgtc gatgtttttt tccgtgtgag gaaaatagtc
      421 ggcgtaatcc acttcggaat cggccagacg gcgcagcttg tgaacggtca tttgcggctt
      481 gttgcgaaaa atctgcacaa gccccttcac ctttacgaag tcgtcccggt cgaaggtttc
      541 cattacctcc ggcacgccgt cccacatttt ggcgtcgagc gtcccggtgc ggtcggcgag
      601 cgtcaggcac agaaacggct cgccggtttt cttgagccga acctccttgt ggtgaacgag
      661 gaaaaacgag gtcacgacct cgttaggctg gagatcgcgg ataaaatgag ttttcaagta
      721 ggccttctag cgccgcccgc tactgcggct gcctgcctcc gttcgccgga ctgctgctga
      781 cgccaggcgt actcggaggc ggcgcagagc cgccggccgg agccggcgtt gtagagctat
      841 tccctcgcgg cactacccag aagagcttct ttttcttttt cgtcacctct tccttggttg
      901 tgacggctgg cttgaagttc tccggatcct tccacgtggt gtcccggccg ggcgcggcgc
      961 cggtgtccac atagccggga cgaatctcga tgaaagcggc ttcccgcagc ttggtgagat
     1021 acgcgcgcag cgccggctgc attttaggca tgaataaccg ttcctgaatt tcctgctcga
     1081 cgtcttccag cgaggccagc ccagcctgat tccgcgcctc tacttgcaga atcaagaagc
     1141 cgttccgccg tttgagcacc tcggtaatgt agccgcgctt ggcgttgaaa acgatgtctt
     1201 cgatctcttt gtcgagcagc ccgcgtttcc agaagccgat gtcgcccccc gcccgggctg
     1261 attcctggtc gtccgagtat ttgctcgcca cttcggcgaa gcgctcgcct ttgcgaaggc
     1321 gggccaggat ttcattcgct tttttctcca gcgctggtat atctttcggg tccttgccct
     1381 ccgtcgagat cagaatttgc cggagccgaa cctgctcggg gcgtacaaaa tcctgtttgt
     1441 gttcctcgta atatttcgct acctcttcct tggtgaccac aatgtggctt ccgacttcct
     1501 gctggatcac gcgctcggtg agcaactggt tgcgaacctg gtctttgaga tcctcataag
     1561 gcattccacc tttttcggag acgaacttct cgaactcctc catggtgggg atgttatatt
     1621 gacgccgcag atcatcgagc cgcttgacaa ccgccgagtc cacattaacg ttgagttcct
     1681 tggcgcgctg caccagcaaa ctctggtcga tcaagtcccg cagggcgttt ttttcatgtt
     1741 ccgccaccgc gttttcaaac tgctgcgtgg tcagcttctg ccgcgcaagc tccgcccttg
     1801 cttccgcgtt gctccgctcc agctccgacc gtgtgacaat ctcgccgttg attttggcga
     1861 tgatctcctc caacacctga acgctggcag cggcgcccag caaggcggcc cagatgagac
     1921 caaaaacaag aaagcaggac ttcttcatac cgacattgta actcccagcc tttgggggcc
     1981 ggcttcgaga tcgggcgtct gctgcctgtc gccggcggct ggatctattt tcgtgaacaa
     2041 gagcggtaga tgcaagagct ttcgggcctc gaaaagaagc ccgcctttcg ttttttcgcc
     2101 cccggggggc gcgccccgcg agcgcgatgc gcggccgtgc cacgcaattc cgccgcccgt
     2161 gtcccccccc gagccgaccc cgaaccgcgc gtgtcatcag gcctgcaact gcttcaagag
     2221 ctccgtaatt tcctggatgg tgtgagcagc gcctccgcca ttcatcgtaa agcggagcac
     2281 gccggcgggc gtaaattccg ccccgggcgc gccggcggcg aacgccatta gcttctgtgg
     2341 atcgaccgag gcggcctcgt tgaatttgat cgagatgcgg tcctgccggc ggtcaatcgc
     2401 atccacgcga atcctttcgg cgatcatctt caacgcggca tactccagca aattggccac
     2461 tccctcgggc agcggaccgt agcggtccac gagctcttgc cggattttct cccgctcctc
     2521 ttcgctttgc aggccggcga tgcgcttata cgtgcgaagg cgctggttct cttccgggat
     2581 gtactcggga ggaatgcgga cgtccaggtg caaattgatc gaaaccggtc cggccgccat
     2641 cacctgttcg ccccgcagtt ctcgcacggt gttttcgagc atctgacaat acatctcgaa
     2701 gccgacggcg gcgatgtggc cgtgctgttc tccgcccaaa aggttgccgg agccgcgcag
     2761 ttcgaggtcg agcgcagcaa tcttgaatcc cgagcccagc tcgctgaatt ccttcaaggc
     2821 tgcaaggcgc tttttcgcca gcggagtcaa ctcggcatct tccggcacga gcagatacgc
     2881 ataggcgcgg cggttgctcc ggccgacccg gccccggagt tggtacagat cggagagccc
     2941 aaaccggtcg gcgcggttga tgatgatggt attcgccaag gggatatcca gcccattctc
     3001 gatgatcgtc gtcgaaacca ggatgtcgta ctcgtgatgc atgaacttga gcatgatctt
     3061 ctcgagttcg cgctctgaca tctgcccgtg gcccacgccg attcgggcgc gcggaacaag
     3121 cgtttgcagc aaggaggcaa tcgaccagat gctttccacc cggttgtgga ggaagtaaac
     3181 ctggcctccg cgctccagtt cctgctcgat agctgtgcgc aaaagctttt cattgaaagg
     3241 ggccacaacg gtctggatgg ccagccggtc tttcggcgcg gtttcgatca ccgacatatc
     3301 gcgcaagccc accagcgaca tatgcagggt gcggggaatc ggcgtagccg tgagcgtcag
     3361 cacatcgatg ccctttttca tttgcttgag gcgctctttg tgccggacgc cgaatcgctg
     3421 ctcctcgtcg atgatcagca atcccagatc gcgaaacacc acatcggcgg aaagcagcct
     3481 atgcgtaccg atcaagatgt cgatctttcc atcggccaag tcggccagaa tttgttcgat
     3541 ctccttcgtc gagcggaagc ggcttagcat ctcgatcttt actggaaacc gcgagaagcg
     3601 ctgcttgaag gtctcgaagt gctgaaaggc gagcaccgtt gtcggggtga gcacagcgac
     3661 ctgcttgccg tcgcccaggg ctttgaaagc cgcccgcatg gcgacttcgg tcttgccaaa
     3721 tccgacatcg ccgcagacca gccggtccat ggggctttcc gattccatgt cgcgcttgat
     3781 gtctcgtatg gccgccaact ggtcctcggt ttcggtgaac tcgaaagcgt cttcgaactc
     3841 gcgctgccag ttgctgtccg gtgagaaccg gaaaccggtg gccagcttgc gctgggcgta
     3901 cagccgcaac agctcttcgg ccatgtcccg cattcgcgcc ttgatacgcg tcttggtccg
     3961 cgtccaagtg gcgccgccca ggcggtccag ttgcgggcgg acgccttcgg gcgcccggta
     4021 tttctgcacc agatcgagcc gggatagcgg cacgtacagt ttggcgttct ccgcgtattc
     4081 caacgccatg aattcttccc gctggccgtt gtctccgatc tgttgcatcc cggtatagcg
     4141 cccgataccg tgctccacat gcaccacgta gtcgccgggc ttgaggtctt gaaagtcaga
     4201 gagaaacgtg gagaggcgcg atttgcccga gggcggcttg gcgacgaact ccgaaacttc
     4261 aaacaaatcg tcggagccat gcacagccag ttgggaatcc gggaaacgga ctccgcggcg
     4321 cacggcgccc tgcaccagcg tgacgctcgc aaattcccct gccagatagg ccttttcctc
     4381 caggtaccgc gcggccgcgg aacagggatc cttgagccca agctggaaac tgacgccata
     4441 ctcgttgaaa atgtcagcca ggcgttccac ctctcccgct gtggcgccaa aaaaaacggc
     4501 gcgccagcct tgctggatct ccgactccaa ttcgcgcatg caatgcggaa tattgccctg
     4561 aaagcgcgtg gccggaccgg tggcaatgtg aaagctgccg ggggagacat ccagcccgag
     4621 ctcttccacg ctgacctggg ggaagcgcgc agcctgttcc tgccattccc gcaccgccca
     4681 gtaatagctg gccggagggt ccaggatttg ctcggatttc ggatacgccg cctcaagctg
     4741 ctgccaaagt ttttccgccg cggccaggac ctcggagagc ccgtcaaaca caagcagaca
     4801 gtcgggcgcc aggtccagaa gggatcccgt gagaggaccg gcgggagtgg acgtctcttc
     4861 cgccaaaggc agtaagagcg cttcgtctac agacaggacg gagcgctggg tctcgatatc
     4921 gaagcgccgc agcgactcga tttgatcccc gaagaactcg attcgcaccg gccgtgcgct
     4981 ttcgggcgaa tacacgtcca gaatgccgcc gcgcaccgag tattgcccca ccatttcgac
     5041 gggctcgtgc cgctgatagc cgatccgatc gagctcggca atcaccaaat cgagcggcat
     5101 ttcttcgccg cgggcgagac ggaaggcaag gccatggtag tattccggcg cgtgaagttt
     5161 ccgcaaggcg gccgcaattg tggtaagcac aagcgggggg cgctcgctcg acagccgcca
     5221 aaggccgatc gcacgccgtt cgctgatctc cgggtgaggc gccagcccat catagggcag
     5281 cacatcgtga gcaggcagcg taacggggcc ggcttgccgg tcggagaaaa gcaggcggta
     5341 gaacgtctcg atcggctcgg ccagcagatc cgcttccttg tcggaaggaa ccaggaccag
     5401 aactgtgcga tccagagcct gatgcaggag caccgaaaac aaggctcgcg ccaccgggac
     5461 cagccccgac aattgcaggc tgcgggcggc cttcctgcgg atttctttga gaagctcctg
     5521 aaagctggga tggcgttcca gccccagaaa cagatcgcgg acgaatggaa gtgtcaacgc
     5581 tgctgttcca aaacggtttc aactattcca gtagtggaaa aatcgggttc gagcggcaga
     5641 gccaggacct gacctccggc ggcttccacg atctcccgcc cagcgatcca gtgggaccaa
     5701 tcggctcctt taaccagcac gtccggcagc aacagggaaa gcagctcgca gggagtttcc
     5761 tcgtcgaaga tggttacagc gtccaccgcc tccagggatg ccagcaattc ggctcgctcg
     5821 tcctggccga tgatcggccg taagggtccc ttgagacccc gtacgctgga atcgctattg
     5881 atcgccacga tcagccggtc gccgaggctt ctcgcccgtt ccagcaggcg aatgtgcccc
     5941 gggtggagca agtcgtagca gccgtttgta agtacagttt tctgggatct ttccttccag
     6001 cggcggcgtt cggccagaag ctcgtcgcgg tccagtaccc tgcccacaaa tcttatccta
     6061 acatcagcaa accagcggcg agatgcgcca gccggcgtga cggcatggcc caccggcgcg
     6121 agaaagttct actgctttgt ccccctggag cttcagggta acgccctatc gatgccgcct
     6181 gggacggctg ataatctaac gtggtggcga ctttggctgc ggcaccccag acgttccgtc
     6241 ccagcaaccc ggtcacgctg caagacgcgg gtctcaatca aaatctactc atcgatctga
     6301 tgctcaagct cactctgctc gagggtgaga ccacgctcca ccggctctcg agccagatga
     6361 aggtcagcat gaccatcatg gaccagctct ttcgctactt ccgccgcgag cagttgtgcg
     6421 aggtgaaggg catggtgggg aacgactatg cgctctctct ctccgcggcg ggacgaaaga
     6481 tcgcccagga gagatactcg atcaaccaat atatcgggcc tgcgccggtg aagctgaatg
     6541 actactgcga agcggtcaag gcgcaggcgg cgcaccatcc ggtctcgcgc tcgatgctct
     6601 cagaggtgtt ctttgatctg gtgttgactg aacccgtcct cgaccagatc ggcccggcca
     6661 tcatttcagg caccacaatc ttcctttacg ggtccaccgg aaacggaaag accagtattg
     6721 ccgagcgcct ggtgcgcatt tacaaggatt acgtttacat cccgtacgcc atcgaggtcg
     6781 agggacagat cataaacatt tacgactcgg tggtgcacca ggccctggag acgcaggaag
     6841 acgatacgga cccgcgctgg ctgctctgcc ggcgcccttg catcgcggtg ggcggcgaac
     6901 tggtacccga gatgttggag cttcggtttg acgacaatct gggcttctac tcggcgccgc
     6961 tgcaaatgaa agccaacaac ggcctgttca tcatcgatga cttcggccgg cagatgatca
     7021 acccccgcga tctgctgaac cgctggatcg tccctctcga ccggcgggtg gattttctat
     7081 cactgcgctc gggcgtcaaa ttccccattc ccttcgagac tctggtcgtg ttctccacga
     7141 acctcgatcc gcgccaactg gccgacgaag ccttccttcg gcggattcag aataagatta
     7201 aagtggacgc catcactccc gagatcttcg acaaaattct ggagcgcgtc tgcgaggagt
     7261 accagattcc cttcgatcct gaaatggccc aatatctgcg cgagaagtgc aaccccgacg
     7321 gcaaaggcgt gttgcgggct tgctatccgc gagacatggt gcgcatcctg caaagcatcg
     7381 ccgactatga aaaccgggcg ccccagctca cgaaaaaaga cttggatcgc gccttgaatc
     7441 tgtacttcgc aaaaacctga gaacggcagc ccatattgcc gcccgggcac agaggtaaac
     7501 cgcccgccaa aattcgccag aacgtttctc cccgatagta agatggcaaa gtgaacctcg
     7561 actttccgaa ggctgagatc catttgcatc tggaaggaac gatcgagccg caaacgcttt
     7621 gcgagctggc ggcgcggcgg catgtcgcgc tcaccgaaga ggaagtagcg ggccggtacc
     7681 gctatgcgga cctcctgggg ttcttgcagg ctttcaagtg ggtgacgcaa tacttgcaaa
     7741 ctccggaaga ctaccagatc gcggccttac gaatgttcga aaaactgcac cggcaaaatg
     7801 tggtttatgc ggaggtctac ttcagcgcgg gcatctgcct gtggaagggg atggaagtgg
     7861 atcccatctt cgatgcgctc gaggaggcgc ggcggcgggc cgaggccgac tggggcattc
     7921 gcgcccggtg gatctttgat gctgttcggc agttcggaat cgaagccgcc gaacgcgtat
     7981 tccggctggc cgcacgctgg cagcaccgac atgtggtcgg cgtcggaatc ggcggcgacg
     8041 aaaagcgggg agcgccggcg atgtttcaaa cgctgttcga agccgcccgc tccgcgggac
     8101 tccggcttct ggcgcacgct ggagaaaata cagggccgga gtttatttgg gagagcatcc
     8161 gaaagctcgg cgcgcagcgg atcggccacg gcgtttcggc gatttgcgat cccgccctgg
     8221 tccagtatct tcgagatacg cagatcccca ttgaagtttg cgtgacgagc aactacgcga
     8281 ccggcgtcgt tgccccaggc gcggagcacc cggtccggcg gatgttcgac gcaggcctgc
     8341 tggtcgttat caattccgat gatcctgcca tgttcggcac gaccttgaat gacgagtatc
     8401 ggcggctggt cgagcggcac ggtttcaccg aagccgagat cgctgtgctc gcccaaaaca
     8461 gttttcaggc cgcgtttcta acagagcagg aaaggcaaga gttcctgaag gcttaagcgg
     8521 caaacgcgcg cgacccggca acgtgccggc cgttcagcgc ccgcgctacg tatcggctcc
     8581 cccgccggct tggccggaac ggcaaaagac tactggttgg aactgggggc gctcgccccg
     8641 ggcgctccga gggcagaatg gccggctggt atcgtgattt cgaagtccga ctggaacttc
     8701 cgataattgc cgtaaaggat ttctcccccg gcgtgcacgg tcctgaccag cgccacgcgg
     8761 ccgctgcccg cataggagat ccgatgcggc agccagactt cgtcattcac tttggcctgc
     8821 tcaaactcga ggcgggagcc gggggccagc cgggccagaa agacgccgaa cgcgattgta
     8881 tcagtcgtct cggcctctcc tttcacccat tgcaactccg ctttatcgat ccaaagcttc
     8941 ccgcgcagct tcgtgaaaag ccgcgcttcg ctgttgcgcg ggcgatagcc gggcttgggc
     9001 gccgcctcga ttacgtacgc aggccggccg tgtagcatct cttctcccaa caggcgaaaa
     9061 tcgaaggcat ccggcacttc ccgcaaaaat gcccaggtgc gttcccgccg cttttcgtac
     9121 tccgaggcgc gccgctgcct ctcggtttgc gattgccgct gcgatgcgga aaaagctctg
     9181 ccgagcttct tctcttcctc tctggcctct tccgccggga gcgggcggcc gtccctcgcc
     9241 accagtctgc gatagggcag gccaaacaga aaagtaattt cgtaggtttt ggaggaaacg
     9301 gacctctccc gcccggccgc gtcaagcttg cgttctacga cgcgttcggt gaacgtatat
     9361 tcgcgggcgg cgcggaagtt gcgctcgtca agaacggtcg aacgccgaat gatgtcacgg
     9421 gcgctgtcgg ccccagggtc cgcaggcgga gttgcggtct gatcggcggc gcccagagcg
     9481 attgccgcaa aaagagtgaa cagcgcaccc atcactctgg tagacgcggg gcattgcggg
     9541 cggtttcgcc aggcatcggt tctccagtga aactgccaga cgctgcaata cggtaccctg
     9601 gagagcttgc aacttcttct tcgcccgccc tggggcaagg tgtatgtacc tgcttccgcc
     9661 atccggcggg ttgttctctg aaacaccatg cactggggtt gcgcgctgca cgcacagccc
     9721 aggttcccca cagtagcaaa ctgtggccgc aagacgccgc tctcgctggg aacggggcaa
     9781 gctggcttcc tggcatacgc cgcaattcgg agacgcgcca tttcttttcg ctattccttt
     9841 tcaccaagga taatatggat cggttcggct tctaagcagc gccgggcggc gcaacgcata
     9901 aaggacccag tcgtggtaaa cagccgatgc agcgagccac gccgtcggaa gaaaagaagt
     9961 cggagattcg caaattcaaa accacgaaaa cgatcatctg gagggcgcta ttgagttggc
    10021 aatttgagtt gatggatccg ccgtacggag ccgtcagtga aggaccggtc tggacgggct
    10081 cgaagttgct cttcacccac attcaagaga gccggattct gcaatatgat cccgccacgg
    10141 gtttgtgcgc cgtgtttcgc gacggcacga actgcgcgaa tgggctggcc ctggaccagc
    10201 aaggccgtct ctatggatgc gagggaggcg ccacggccga tgcgcgccgg gtcgtccggt
    10261 acgaggcggg gggcggcgtc acggtgcttg ccgacagctt tgagggaaag cgcctgaata
    10321 tcccgaatga tctggtgctg gatccgcagg gccgcgtctg gtttacagat cctttttacg
    10381 aaggggcggc cggaccctgg agcttcgatc gcgccaacaa agagctcaac cacgattcgg
    10441 tgtatcgcct ggacccgcag ccggacggat cgtatagcat ccaccgagtc accttcgaca
    10501 ccaccagacc caacgggttg ctgttctcgc tggattacca gacgctctac gtcgcccaga
    10561 gcggccgcct cccggaggaa aagcggcagc tccgcgcgta tccggtgcgg agcgacggac
    10621 ggttgggggc ggccgaagtg ctgcatgatt tcggcgagca tcggggcatc gatggaatgc
    10681 gcctggatgt cgaggggaat atcatcgcca cagcgggttg ggaactgggc ggccctgggc
    10741 cgtcgatcta cgtcttttcc gcatcaggag aagtgctcga gcggcacgcg gtcccggcca
    10801 aacggccgac caactgctgt ttcggaggcg gcgaactgag cgatttgtac gtgacgacga
    10861 cggaaggaca tctgtttcgg gcgcacaccg gccgctgcgg ccggcgatac ccgtgaagca
    10921 aaccgcagcc ggggattgat gcttggccgg atcaaggcaa gtccctagag gaagaattgt
    10981 cagtaacagt acggaagccg gcgacgaaaa ccgccgttcc ggcgcgcaag ccgcagccgc
    11041 ataattcttc ttgttcgccg gatcactctc ccttacaatt gagcgttccc cagttgaatc
    11101 gtcccccatg gtgttctcga aaggaggcgt caagatgtca gccacggcgg cagtcactgc
    11161 aaccgatcgg aacctcgagg atcagatctt tcaattgcaa aagatggatc tcgattcgcg
    11221 tgtcaaagaa atagtcgaag aagcggccca gctttcgatg caaggccggc atctggaagc
    11281 cgcagcgctg gtagaaaagg ccgaagcgat gcgggcggca attgcggctg aaacgccgtc
    11341 ggccggactg ccccccgcgg aaacgcagcc gcccgcagaa ggcgagcagg tggtcgcccg
    11401 cctggtgggc gatgtagcca acgggttagc caaagtcctc gtttcggcgc tgcaagattt
    11461 agaacaacat atcgctggtg aaacgcgaaa gcttgctacg tctttcaacc aacagctaga
    11521 ccggctgcaa acaacggtgg agagcttgct gccgctcggc gaacggatcg agcaactggc
    11581 cggcgccatt gccgaacaaa aggcggtggg gctcgccgct cacgagaagt gcagccaact
    11641 ggcggcggag actgcgctcc tacgagaggc tgacggccgg tacgagaacg ctctcggcag
    11701 ccttcgcgcc gaaatgcagg agaggacggc ttccgtctca gagcgcctgg aggcgctaac
    11761 gggccgcctc ggccttcagc aggaagaaat ttcagccgtg aaatccagcg tctcggatct
    11821 ctcaccccgg gtagcgacgc tcgtggaaag gctcgaccgg caagccgaag caatccggtg
    11881 cctctacgaa acggagtctc accggggagc tgcgatggac gagctcgtca atgccatggg
    11941 ccgcctcagg gccttgcacc tgccaccgcc tgagcagccc gcaactcaac tgtaatcagc
    12001 accgcgggga agcggcttta ctcggaaatc gcatccttgg gacggcttcg cgggtcttgc
    12061 tcagatgaac ggagccgtct tggctttgcg attttaccgc ttccggcagt tagttcaacg
    12121 cccgcgccgg accgcgctac catcaggatc gctcttggag aagttgagtt ctctgagcgg
    12181 aacactatac tcggaggaga ggcattcctt atacggtttt ccctcgagta ctggatagca
    12241 ctgcttcttc tcgtgctgat cttcccggct tatcctcagg tcacggagat atatacatcc
    12301 acgcgctcgc ttatcggccg cgtgcaagcc gatctcaagc gcgccgaaga tttcgtgccg
    12361 ccgagccaga aggagagaga aagatatgag aacgcgcagc ggcatctgtc cgagctcgac
    12421 cgggagctga gtaaaggcaa gttcgacaaa gaaaagttgg actctgccat cgacgacgtg
    12481 aaaaatgtgg ttgaaaacaa tacgctgacg ccggactctc gcgacgccct taccgccgat
    12541 cttcgcagct tgcgagagct tcgcgccaag cactagcgcc gcccgtggat tctcgttccg
    12601 gcggccggat tggcagttgc ggcaaccgag gtcacccggt ccggctggac ttgggcgcgg
    12661 cgggggcctt gttcgccatc tcgatccggg ttacactttg accgccgttt tctatgtcga
    12721 ccagcagtat gcgccgatat ctccacctgc tgtggctccc ggttctcggg ctgggcgctt
    12781 gccgggctgc ctcggttccg gtggacttgt cggcagttcg cccggggccg gtatcggtgg
    12841 aggcgaaaga aaaatctctg cttgtgcgct ggccggatga gagctcgcgg ctctggcgcg
    12901 cggagttttc tctcgatccg tccgaacctc tcatcactga aatcgcagca ggcgacaaga
    12961 aagtgattac gggcgcagtt cccttgtatt ggtgcgaaag cgggaagcgg cggggcggct
    13021 gggataattt tttcgacgcg ccggcgacgc atcccgaggg catgcgccgg tgggtggggc
    13081 gattcaagct gacttctgtc aaagcgcgta ccatcggcga tcgtgttgaa gtcgccttta
    13141 acggccttcg gctgggcgtt tttcggggag cggtggccta tatcttttat ccagggagcc
    13201 ggttgatcca gcaggaagcg gttgtttcca cgcgcgagcc tgacacggcc tatttttacg
    13261 acgccggact ccagatgttg atcccatcgg aacgcacacc gggcctcttc agcatggatg
    13321 cccgggcgac gtattacgac accagcgggg agcttcgaac tgaggcgtcg gattatgagc
    13381 gcaaggcggt tgcggcccgg taccggacga tcgcagccgc cacgccggcg gggagcgtcg
    13441 ccgtttttcc gccgccgcat caatattttt ccgctcggga cgtaactaca aacctccgat
    13501 acgtatggca tgccgcctgg ggcggaaaag tggcgatcgg gatccggcaa atcgccgatg
    13561 acaacagtcc gtataacccg ggaatcaacg cgcctccggg cgcccagcag cgcatgagcc
    13621 tttttctgct ggtgacggag ggcgatgcgg caactgctct gcgtgattcc ctccgctaca
    13681 ctaaccgcga ccgttttcca gccctggagg ggtatcaaac cctcagcgct cactggcatt
    13741 gggcctacac catgcaagct ctccagcacg gcccggattg gattccaccc ttcaagcccg
    13801 tgctgaaggc gatgggagta gaggccgcga tagacatgga tttccatggc gacggccacc
    13861 ccagagactt gagcgaaacg cggctcaagg agctggacgc ttttttccgg gcctgccgcg
    13921 ctcagtcgga ctcggaattc ttgctgatcc cgtccgagga agccaatgtg tatctgggcg
    13981 ggcactgggc gctgatattt ccgcatccgg tttactggtt tatgagccgc cctgaaggga
    14041 cggagtttct cagccaggat gccaggtatg gcccggtcta ccacattgga agcgccgccg
    14101 acatgcttga aatgattcgt cgggaaggcg gcattctcta ccagacgcat ccgcggacca
    14161 aaggctcgac cggatacccg gacaaaattc taaagaccga ttacttccgc gatccccgtt
    14221 atgtgggcgc tggatggaag gcgatgccct ccgatctttc ctcgtttcgg ctcggccggc
    14281 gcgcgttcgg ggttctggac gacctgaata actggggctt tcggaaacgc ttgctcggcg
    14341 aagtggatgt gttccagatc gattccaccc acgagcttta cggccacatg aatgtaaatt
    14401 acgtccgcct cgcccgtttg ccctcctacg acaactacgg ccagatcctg gatgccgtcg
    14461 ctaaaggcga ttactttgtg accaccgggg agatcctgct gccagaggtg agcatttccg
    14521 ggcaaggtgg cgaaattctg gttcgagccg ccgctcgctg gacctttcca ttacagtttg
    14581 ccgaggtcgt gtggggagac ggctcggaaa caacgcataa aacgtttcct ttggaaacca
    14641 cccgagaatt cggttctgga acctttgaat ggaaggtgga tgcgcccggc tggaagtggg
    14701 cgcgtctggc cgtctgggac gccgccggga acggggcatt cgtcaatccg gcttggaaat
    14761 aacccccgcg acagattggc cggtggccag acccggtgaa ttgcggcctg cctccgtaac
    14821 aacgtccgca gaaaagccgt tcattttcga aattacagaa ctcttacaaa catttgcgga
    14881 aattgctgtt aggttggaat ttgagcctta tccgggagac cccttgagca cccaagtcat
    14941 taacatatct aatcgaagca tcaccgccca ccagacgatg aactggccca cgatcatcgc
    15001 cctggtattt ttccatgccg gggccgtcgc tgcgctgttc gtgtttaatt ggcgcgcttt
    15061 tctggcggca ggttttcttt attggctggc catcggcgtt gggattagtc tgggctatca
    15121 tcgcctgcat acgcaccgtt cgtacaaggt tccattgttt ttggaatatt tctttgccct
    15181 ttgcggctct ctcacgctgc aaggaggccc aatttcctgg gtggccactc atcggattca
    15241 tcaccagaaa tccgatcagc ccggcgaccc gcactcgccc cgggacggcg ggtggtgggc
    15301 gcatgtgggt tggattcttt tcggcgaagc cgtacataac aacacccggg ccatgtcgaa
    15361 gtacgcgccg gatctggcga ggcaccggtt ctaccggtgg ctgaacaatt atcattgggt
    15421 tccgatggtg gttctgggct ttatactgct cgccgccggc ggcctcccga tgatgctgtg
    15481 gggcatctgc gcccgcgtag tggtcggcct gcacacgacc tggctggtga actcggctac
    15541 tcacatgtgg ggaaggcggc gatttgccac ccgcgacgac tcccgcaaca gttggtgggt
    15601 ggctttgctg accttcggcg aaggctggca caacaatcat catgctcatc ccacttcggc
    15661 gaggcacggg cttgcgtggt atgagtttga cccttcctgg atcgaaatca aagttctgga
    15721 actgctgggc attgcgcggt cggttcgcgt ggcgaggctg gatagcgtgc ttcccaagca
    15781 taactcggcc tgagcgcagg atcttcaggg ctgaatcgcc ctgccgcacg cgagcggatt
    15841 taccggcttg cccgcaaaat ccgcgtcccc gcattaaact ggatttatgg tggtagcggg
    15901 ccggcagaaa tcgattgcct ttttcatcgc tctcggcgcg ggcctggttg tcgtcattct
    15961 cctcctctac attggttggg tccttctcaa ctggcgtcac ggcattctgc tgtttctggg
    16021 cgccctcctg ctggcgatga ttattgccgg agtcgtggta aacacgacgt ttctggtacg
    16081 ggaaatccgc cggaatgccc agcacgacgc tttcatcaac gccgtcacgc acgagctgaa
    16141 gaccccggtc gcatctatcc ggctgtatct gcaaacattg cagacacggg aagtggacga
    16201 agggaaaagg cgcgagttct accggaccat gctggaagac agcgagcgcc ttctggcgac
    16261 gatcgagcag gtattgcgta caggccgcat cggcgcctca ggccgtaagc tgcatcgctc
    16321 gccgatcgat ctgggcgggg ttatcgaaga atgcgtctca cgggcccgca cgctctaccg
    16381 cctctccgcc gagtcgctcc aataccatcc gggccctgcg gttacgattt tgggagatgt
    16441 cgacgaagtg caggcagctg tgttgaacct gattgacaac gcggtcaagt attcggggaa
    16501 caacgtcaag gtaattgtcg aaaccgccaa gaatggaaac tatgtgtccg tgcgggtcaa
    16561 agatcagggg ccgggtattc cggtaagcga gttgaagcag atttttaagc ggttttatcg
    16621 ggcgccggga tccctcgcca cccgcgtaaa aggaaccggt ctcggcctgt atatcgtccg
    16681 ctccgtggcc aagcggcacg ggggcagcgc gtgggcggag agcgaagggc ccgggcgcgg
    16741 cagcacattc gtgcttcagc ttccgatatt gaaatgatcc acatcctggt cgtcgaagac
    16801 gagcagcatc tcgccgaagg ccttcgtttc aatctcgagg cggagaacta tgaggtcacc
    16861 gtcgtggata ccggggaggc ggcgctcgaa gcgctcaagg aaggggaatc gcagcacttt
    16921 gacgttgtgg tgctcgacgt gatgctcccc ggcaaagacg gcttcgccgt aatctcggaa
    16981 ttgcgccaga gcgggcaatt catcccgact ctgatgctga ccgcgcgcgg ccaccccgac
    17041 gacgtgctga aggggtttgc ggcgggcgcc gacgactatc tcaccaagcc gttcgaattg
    17101 cccattctga ttgcgcgcat acgtggtctg ctgcggcgcc gcgactggct cctggggtct
    17161 gtcaacagcg ctaatactcc ggccgctcgc ggcgatacgt tcaagttcgg cgacaagtcc
    17221 gtcaacttcg accttttgga attgcacgtc cgcgaccagg tgtttcccct gactctgatg
    17281 gaaatgaacg tgctgcgcta cctcatccag cacgagggcc ggcctgtgtc gcgaaaagcc
    17341 atgctcgaag aggtctgggg ccttcacgag gacaccgaca cccgcgcgat tgacaacttt
    17401 atcgtgcggc tgcgccgtta tatcgaagag gatccgactc gaccgcgcca cttgctcact
    17461 gtgcggggcg tgggctaccg attcgtggcc gccccggtga aaagcggcta aaactctcca
    17521 acaagacgcc gaaatcgttc ggccttgtcc agccgatcct gccggcgcca gcgccgcgca
    17581 ggacaaaacc atttcgcgcg ggaagaatcg gcagtgcgct aactctgcgg agccggcctg
    17641 gcggcagcct cgagagcagg ccggcggatc gcttgaataa cgcattaccg gcgacccatc
    17701 gctccggctt atctacgcgc tgaaggtagg gtagaatcgg atcacctatg aagctccccg
    17761 cgattcgtat tcccgccttc accgcgcttt tggcagtgtg gtcggcgccg ggcgtggcgc
    17821 aagctccccc acggccgcgg atcacgggcc tggcgcatgt ggcgctgtac acgcacgata
    17881 tcgaaaaagc tcgctggttt tacaaagatt tcctgggatt cagcgagcct tttctgctca
    17941 ataaccagga tggcagcctt gctctgacct tcctgaagat caatgaccgc caatacatcg
    18001 agctgtttcc ggaaataaaa ccacagaccg accggctcaa ccatatctct ttcgaggtgg
    18061 acgacgcaga ggcgatgcgc gtgtatctcg ggtcccgcgg cattccagtg ccggaaaaga
    18121 cccccaaggg gcgcatcggg aactcgaatt tcaacgtaaa agaccccgat ggccacacag
    18181 tcgagatcgt gcaatacgag tccgacggct ggagcatgcg cgacaaaggg aagtatatga
    18241 acgaagcccg cgtttcccgg cacatgatgc acgcgggaat tctggtgggc gcgctggagc
    18301 cggcgatgag gttttatagg gacatcctgg gcttccggga gatatggcgc ggcagcagca
    18361 acggcaaaca gcttagctgg gtgaacatgc aagtgcccga cggcacggac tacatcgagt
    18421 ttatgttgta taacgaattg ccagcccccg cgtcccgcgg cgtgcagcac cacatcgccc
    18481 tggagacgcc cgacatggcg ctagcggcgg cgctgcttga agcgacgccg gcccgcaagg
    18541 attacgcgcg accgatggag attcgcattg gcgtgaaccg gaaacggcaa atgaatctct
    18601 acgaccctga cggcacgcgg atagagctga tggaaccgaa aaccatcgat ggcgcgccgg
    18661 cgccgccgtc cacagcgccc ccgccgagat aaacagcgtc cctgttggcg ggaggtgttg
    18721 cgatctggtg ttaattgttg tctcaatcct ctctgcaacc tactttggga gccttggcct
    18781 tttgcacgtc aatcctgcgc cgtgttgtct gggccctccg ccggctggcg ccgtataggg
    18841 gattcgcgtc atctgaaact catcgagaag gcctgaagcg cagggagtcg gctaccagcg
    18901 agatccagca aagcgccacg gcctcaaagg cgtgaggaaa aactggttac ctggtgcgcc
    18961 cggagagatt cgaactcccg accttctggt tcgtagccag acgctctatc caactgagct
    19021 acgggcgcgc ttccaggaca gtattttcat gctaccagac gcctggcggg cgcgctacag
    19081 cacccctccc aacctgtcgt tggtcggaat ttctccgggc cggcctgcat cgttttctcc
    19141 tgcacgcccg gcgtgagaca atagcggtta gcaagccatt gcggccgcat cccgactaag
    19201 ataagtctta tgagcgctcc gtcccgcagg catgtcctga tcgtagacga cgaagagagc
    19261 cagcgccagg cgctggcaac gttggtcgcc agttggggtt ataccccgca aacggccgag
    19321 gacggccaag acgctttgga taagctggcc gccttcccgg cgcaggtcat cgtcaccgat
    19381 ctgagcatgc cgcgcctgga cggcttcgct ctgctgcgcc ggctgcgcga gcagggggca
    19441 gcgccgccga caatcgtcct aacagcctac ggcagcatcg agaacgccgt agccactgtg
    19501 catgacctgg gcgcttattg gttcctggag aagccgattc agcccgccgc cctccaggtc
    19561 ttgctcgaaa gggccctgga aaagagcaat ctttcccagg aaaaagagcg attgcagcgg
    19621 cgtctggcct atagcggcga gctggatgag atggtgggcc gctcgcgttt gatgcagcat
    19681 gtattctcca tgatccaaca ggtcgctccc acttccgcct gcgtcttgat cacgggcgaa
    19741 agcggtaccg gcaaagagat ggtagcgcgc gcgatacatc agctaagttc ccggcgtgag
    19801 ggcccgttta tcgcgatcaa ttgcgcggcc ttgccggaaa ccctgatgga gagcgagctg
    19861 ttcgggcacg agaagggcgc tttcaccggg gccgccgagc ggcgtgcggg ttgttttgaa
    19921 ctcgctcagg gggggaccct gctgctggac gaaatcggcg agatgcagcc cctcacacag
    19981 gccaagcttc tgcgcgtgct cgaggatgga cgcgtccgcc ggctgggtgc gaaatccgag
    20041 ttgcaggtgg atgtgcgtgt catagccgcc accaacaagg tgcccgaggt ggctgtggct
    20101 cgcggcgaac tgcgccagga cctctattac cgtttgaacg tattccatat ccagctcccg
    20161 cctctccgcg accggagaga agacatcccg ctgattgcgg aggctctgct ccgcgacctc
    20221 aatcgaaagc attcttgcaa ggtcacgggc ctggacgccg ccgtcctcga gcgtctcgaa
    20281 cggcacgatt ggccggggaa cgtgcgggag ctgcgcaatg tgctggaacg ggcggtgatt
    20341 gtcgccggcg agggggtgat cacgatgaac catttgccgg cggggtttat gacgccgtcg
    20401 caatccccga gccagccgac gtcagatgga gacagtgtcg tactgcgggt gggcgcaacc
    20461 gtcgccgacg cggagaaggg cctgatcttg aagaccctcg agcataccag caataacaag
    20521 acccgcgccg cggagatcct gggcatcagc ctgaaaacat tgcacaacaa gctcaaagaa
    20581 tacggcgcgg cctgaagtcc atgtctttta agcgtcgctt gagcgtttcc attgtggcgc
    20641 tggttttggc gctgacggcg gcgctttcca tcgtgtatct ccagcggctg gtgcggatgc
    20701 agttggattc ggcgctgcaa caggcccgaa tggtggctca gcaggtcgag agcgcgacgc
    20761 tccagacgat ttctgagccg ccgccctcct ccacgctcga ggaaagtctt tccttctggc
    20821 gcaacgccgt acgggttgac gatcaactca aggaagtgct gctgcgttcc atcagcagct
    20881 ttactacaat cgccgaaatt gccgttaccg acgaccaggg ccgaattctg gccggctctg
    20941 tcgagcggga gcgctgggtg gtccggccgg ccttcagcga gcttttg
//
LOCUS       DASREK010000267        10286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1607675, whole genome shotgun sequence.
ACCESSION   DASREK010000267 DASREK010000000
VERSION     DASREK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607675"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1343
                     /locus_tag="VFA54_14500"
     CDS             210..1343
                     /locus_tag="VFA54_14500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZS52073.1"
                     /translation="MRVPQLDLQAQYAPLREEIEQAVLRVLSSQRFILGEQAEALERE
                     VALYTGARHAVACASGSDALLLALMALDVGPGDEVITSPFTFFSTAGSIHRLGAKPVF
                     VDIEPETFNINPKRIAARINSRTRAILPVHLYGRVAHMEEIAAMAGNCQIPLIEDAAQ
                     ALGASQGGRKAGTFGAMGCYSFYPTKNLGAAGSGGMVVTSDDRLAGRLRRLRAHGSER
                     RYHHNEVGINSILDEIQAAVLRVKLKRLDSWNAARREHAAFYDRALRGAVRTPQPGAE
                     GSHIYHRYTIRAADRDALRRWLGERGVETAVYYPVPLHLQKCFAYLGHSAGDFPQAEA
                     AAREVLSLPMYPELTPQGREYVASLVQEWAAERAKAAAPAATR"
     gene            1348..2685
                     /locus_tag="VFA54_14505"
     CDS             1348..2685
                     /locus_tag="VFA54_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZS52074.1"
                     /translation="MSMLKQPLAAETSHAQALSEKIRSRTACVGVIGLGYVGLPLAVE
                     FAQAGFAVTGIDLDPDKVSRINRGQSYIEDVDTGLLAALVSEKKLKATADFSAVAELD
                     TVNICVPTPLRKSKDPDMSYVVAASEQVARLLHPGMLIILESTTYPGTTEELILPILE
                     KGGLREGRDFFLCFSPERVDPGNPEFQTHNIPKVVGGVSPRSAEIAVLLYSQAIETVV
                     PVSSTRVAEMVKLLENTFRMINIGLANEMALMCDRMGINVWEVIEAAATKPFGYMPFY
                     PGPGLGGHCIPVDPFYLSWKSKQAGAEARFIELAGQINGQMPMFVVGKIQSALNEHAK
                     PVKGSHIHIVGVAYKRNVTDTRESPAIDIIALLEKRGAKISYSDPHVPKLRWDGGSLT
                     SQPLEAAAASADCVVIVTDHAAVDYAKLVKIAQLIVDTRNVLKGIQSEKIVRL"
     gene            complement(2784..3716)
                     /locus_tag="VFA54_14510"
     CDS             complement(2784..3716)
                     /locus_tag="VFA54_14510"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HZS52075.1"
                     /translation="MITKLPSGLRALAIALLAGCLSTGRAASKLNVVGSTEDMAALAR
                     EVGGDRVNVVSIAKGYQDPHFVEPKPSFLLSLQRADLLIVVGRQLEIGWLPPLIVESR
                     NPKIQIGATGYLDASLTCEILQIPTVEVTRAMGDVHPVGNPHYWLDPENGRRIAKAIE
                     QKLSQLRPQDAAYFAQRDADFDRRLGEAEKRWDAAMAPYHGEKIVSYHNSWPNFAKRF
                     GLQVVGFVEPKPGIPPTPSHTLELINYMKEQHVKVIVVEPYFEMRTPQSIARQTGAKV
                     LTLLPSVGGVKEVTNYFQLFDYDIKLLIEAFKST"
     gene            complement(3838..4719)
                     /locus_tag="VFA54_14515"
     CDS             complement(3838..4719)
                     /locus_tag="VFA54_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZS52076.1"
                     /translation="MDMLVFLAAPFVASLILTGIHAYLGVHVVERGVIFVDLALAQIA
                     ALGTTIAVLCGMDPHSPGAYWVSLAFTFIGAAVFSTIRGHRARIPQEAIIGISYAVAS
                     AAAILAMSKSIAETEHLKEMLVGNILTVSWTDVEKTAALYGAIGLFHFIFRKKFLLIS
                     MHPREAEAQGMSVRFWDFLFYASFGFVVTSSVSIAGVLLVFCYLIVPSVAAILYAERI
                     GRRLAIGWTMGTLVSALGCILSVEIDLPTGATMVCTFGIVLILMASVRGLVRKLFGAA
                     HEMEANVATVAGNSPVE"
     gene            complement(4729..5664)
                     /locus_tag="VFA54_14520"
     CDS             complement(4729..5664)
                     /locus_tag="VFA54_14520"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HZS52077.1"
                     /translation="MTLLRRYFFGSALIAFLAAAAAAPAAAAKLNVVGTTEDMAALAR
                     EVGGDHVNVDAIARGYQDPHFVEPKPSFLLRLQKADLLVAVGRQLEIGWLPALMTQSR
                     NPKIQIGAPGYLDASLTCDILEIPTVEVTRAMGDVHPVGNPHYWLDPENGRRIAKAIQ
                     EKLSQLDPADAAYFAQRYGDFDRRLAEAEKRWDAKMAPFRGRQVVTYHNSWPNFAKRF
                     GLKVVGYVEPKPGIPPTPSHTLELIAFMKEQKVKAILVEPYFDSRTPESIARQTGAQA
                     LQLMPSVGGVKEIKTYFDLFDYDINLLTGLFSKVT"
     gene            complement(5701..6972)
                     /locus_tag="VFA54_14525"
     CDS             complement(5701..6972)
                     /locus_tag="VFA54_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52078.1"
                     /translation="MIRKILVPFGLWIASASILMAQDTAELLARIKAMEARIQALEAE
                     VRALQSQPPAAAAAPGAPQQPAPEAAPPQAPAAVQAPVTGPGGLPYYGAQTLGNKIFN
                     PEIAAIGTFRGSAGYGASRLSLDPVPAFEMQESELAFQAIVDPYARADVFLSFSEHGV
                     DLEEGYMTFTALPGALQLRAGKMRSAFGKVNTMHLHVLPWIDRPLVTQNLLGGEDGIS
                     DAGLSLSRTIPAPGQVFLEATGQVYRGDSSDVFQSYRKSNISTVDHLRAYRDITESTN
                     LDLGVSYARGHSPLGEALVNQLYGLDATLRWKPLRRAIYRSFIARTEMVWGRAALPVG
                     VSRPFGYYVSGEYQFRQRWFLGGRFDRSDRLTDSAVHDTGGSVLLTYRPSEFSQVRAQ
                     LRRTRYLNEATANELLFQFMFSIGAHGAHPF"
     gene            complement(7103..7390)
                     /locus_tag="VFA54_14530"
     CDS             complement(7103..7390)
                     /locus_tag="VFA54_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52079.1"
                     /translation="MPAPHSKVLHKAVALVALVAMLAIANATVLPGHWHNGPQGRSCD
                     ICRTGHLPSPQPLTRVEIQAPAAVEWRTPSVKLHAVPEPALISRSPRAPPV"
     gene            complement(7689..8756)
                     /gene="galK"
                     /locus_tag="VFA54_14535"
     CDS             complement(7689..8756)
                     /gene="galK"
                     /locus_tag="VFA54_14535"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HZS52080.1"
                     /translation="MRIFCAPGRVNLIGEHTDYNQGFVLPAALNLACRITVFPHDANR
                     LTARSVNLKAEVSWPIDRLERRGNWGDYIAGVAVALRGLGVNPVPAMLEIDSSVPPGA
                     GLSSSAALEAAAALALCALAGKDIPRVDLARACQRAESEFVGVRCGIMDQFVSLLGCK
                     DHALLLDCRSLHYRLVPLPQDCALVMVNTMVRHELASSEYNRRRQECDQAAAMLRSSL
                     REANLAAAERLPEPWRGRARHVISENARLHRFVAALERNDLIRAGELMYESHNSLRDD
                     YGVSCRELDFLVEAARKIDGVIGARMTGGGFGGSTVNLVRTRLVPEFRRRIAARYREH
                     FGMEPEIYVCHAAGGASEETA"
     gene            complement(8753..8944)
                     /locus_tag="VFA54_14540"
     CDS             complement(8753..8944)
                     /locus_tag="VFA54_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52081.1"
                     /translation="MGLDLRYPIGLMFFLLGAILVGYGLLHPDLRAPLTPINLNVDAG
                     LFMLSFGITMAALARRAKK"
     gene            complement(8953..>10286)
                     /locus_tag="VFA54_14545"
     CDS             complement(8953..>10286)
                     /locus_tag="VFA54_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721592.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HZS52082.1"
                     /translation="YLKLRFDEKTRTLNAVTFAVMTVFSSGISMYALGRLFQLLLGWS
                     FTACVMLSAGIVLVYIFLGGLTASIYNEVLQFFLIAIGLSPLAIVCIAKVGGWDGLRS
                     KLPAVMTSSWRYMGSAAENPMGVDAFTMVAGLGFVLSFGYWCTDFLLVQRAMASESMA
                     SARRTPLVAAFPKMFIPFIVILPGLVAVALTRMGGSGYALPMKASGVYDYDQAFPTLL
                     AQFYPSGILGVGITALMASFMSGMAGNVTAFNTVWTFDIYEAHIRSGADDTHYLRVGR
                     IATVAGVLISIAAAYVATIYNNIMDLLQLVFGFVNAPLFATFLLGMFWKRATGHGAFS
                     GLLAGTLAAALTHGLTSAEGKGAWIAHARALPSTMAQNFWIAIVAWTTCFVVTIVVSL
                     LTRPKADEEMRGLVYSLTERPKAAGLCWYERPATLALVVAAILIALNWYFW"
BASE COUNT         2174 a         3100 c         3124 g         1888 t
ORIGIN      
        1 atattcgacc gactcggtga cttccgtcgc gaaccggtcc tgcttcagtg atttgcgggt
       61 aatacgatcc atcggctatg ggcgctcagc cgcgggcggg ccaaagatcc ttgttatcct
      121 agcgaagcgg cccccagaaa agccggcggt gcaagcgcaa caacaagtta ttatagcaag
      181 taatacgcgg ccgccgggcg actcaggata tgagggttcc tcaactggat ttgcaggcgc
      241 aatatgcgcc attgcgggaa gagatcgagc aagctgtgct gcgggtgctg tcttcgcagc
      301 gctttattct gggcgaacaa gccgaggccc tggaacgtga agtggcgctc tacaccggcg
      361 cccggcatgc cgttgcctgc gcctcgggaa gcgatgcgct gctcttggcg ctgatggccc
      421 tggatgtggg tccgggcgat gaagtgatca cctctccttt cacgtttttc tctaccgccg
      481 gctccataca ccgcctgggc gcaaagcccg tgttcgtcga tattgagccg gagacattca
      541 acatcaaccc gaagcgcatc gcggcgcgca tcaactcgcg cactcgcgcc attctacccg
      601 ttcacctcta tggccgggtc gcccatatgg aagagatcgc ggctatggcc gggaactgcc
      661 agattcctct tatcgaagac gccgcgcagg ccctgggcgc cagccagggc ggccgaaaag
      721 ccgggacctt cggagctatg ggttgctata gcttttatcc caccaagaat ctcggagcgg
      781 ccggcagcgg cgggatggtg gtcaccagcg atgaccgcct ggccggacgg ctgcgccgcc
      841 tccgggcgca cggctcggag cgccgttatc accacaacga ggtgggcatc aacagcattc
      901 tcgatgaaat acaggcggcc gtgctgcgag tgaaattgaa acggctcgat tcgtggaatg
      961 cggcccgccg cgaacatgcc gccttctacg accgggcgct gcgaggcgct gtcaggacgc
     1021 cgcagccggg ggcggagggg tctcatatct atcaccgata cacgatccgc gcggcagacc
     1081 gtgacgcgct gcgccgctgg ctcggcgaac gcggcgtgga aaccgccgtc tattaccctg
     1141 taccgctgca tttgcaaaaa tgttttgctt acctgggcca ctcagcggga gattttccgc
     1201 aagccgaggc agcggcgcgc gaagttctct cattgccgat gtacccggag ctgacgcccc
     1261 aggggcgcga gtacgtagca tctttggttc aagagtgggc cgccgagcgg gcgaaagccg
     1321 ctgcgccggc cgccacgagg tgactgcatg tccatgctca aacaaccgct ggcagccgaa
     1381 accagccatg cccaagccct ctccgagaag attcgcagcc gaacggcctg cgtaggggtg
     1441 attggcctgg gctatgtggg cttgcccctg gcagtcgaat ttgcgcaagc cggtttcgcc
     1501 gtgactggca tcgaccttga ccccgacaag gtctcccgaa tcaaccgggg gcagtcctat
     1561 atcgaggatg ttgatacagg cctgctggca gcgctggtat cagaaaaaaa actgaaggcc
     1621 accgccgatt tctccgctgt ggcggagctg gatacggtca acatctgcgt gcccaccccc
     1681 ctgcggaaaa gcaaagaccc cgatatgagc tacgtcgtcg ccgcatccga gcaagtcgcg
     1741 cgccttctgc atccggggat gctgatcatt ctggaatcca caacctatcc aggcaccacg
     1801 gaagagctga tccttcccat tctcgaaaag ggcggcctgc gcgagggccg ggactttttt
     1861 ctctgcttct cgccggagcg agtcgatccg ggcaatcccg aatttcagac gcacaatatt
     1921 ccgaaagtcg tcggaggcgt atcgccccga tcggcggaaa tcgcggtttt gctttactcg
     1981 caagccatcg aaacggttgt gccggtcagc tcgacccgag tcgcggagat ggtgaagctg
     2041 ctcgaaaaca ctttccggat gatcaacatc ggtttggcga acgagatggc cttgatgtgc
     2101 gaccgcatgg gcatcaacgt ctgggaggtg atcgaggctg cggcgaccaa gccctttggg
     2161 tatatgccct tttatcccgg gccaggcctg ggaggtcact gcattccggt cgacccgttt
     2221 tacctttcct ggaagtccaa gcaggcgggc gccgaagcgc ggttcatcga gctggcaggc
     2281 cagatcaacg ggcagatgcc gatgtttgta gtggggaaga tccagtcggc gctgaacgag
     2341 catgcaaagc cggtgaaggg ctcccacatt cacatcgtcg gagtagccta caagcggaac
     2401 gtcaccgaca cccgggagtc gccggccatc gatattatcg ccctgcttga aaagcgggga
     2461 gccaagatca gctacagcga tcctcacgtg ccgaagctgc gatgggatgg cggctcgttg
     2521 acttcacagc cgctagaagc ggcggccgcc agcgccgatt gcgtcgtcat cgtcaccgac
     2581 catgccgccg tggactatgc gaagctggta aaaatcgcgc agctcattgt cgacacgcgc
     2641 aatgtgctga agggaattca atcggaaaaa attgtgcggc tgtgaaaccg cctgccgcct
     2701 atcgagaccc gggtggccat gggggcattc tcgcgagact atacctgccg cgccgaggga
     2761 agaagcgttc ttcgtgagac gattcaggtc gatttgaaag cctcgatgag aagcttgata
     2821 tcgtaatcga acagctggaa gtaattcgtc acctccttca cgccacctac cgagggcagc
     2881 agcgtcagca ccttggctcc cgtctggcgg gcgatggatt ggggcgtgcg catctcaaag
     2941 tacggctcga ccacgatcac ctttacgtgc tgctctttca tatagttgat cagctcgagc
     3001 gtatgagaag gagtcggcgg aataccgggc ttcggctcga caaaaccgac gacttgcagg
     3061 ccaaaccgct tggcgaaatt gggccacgag ttgtgatagc tgacaatctt ttctccgtga
     3121 tagggagcca tcgccgcatc ccatcgtttt tcagcttctc ccaggcggcg atcgaaatca
     3181 gcatcgcgct gcgcaaagta tgcagcgtct tgaggccgga gctgagaaag cttttgctcg
     3241 atggctttcg cgatgcgccg cccgttctcc ggatcaagcc agtagtgggg attgccgacg
     3301 ggatgcacat cgcccatcgc cctggtcacc tcgaccgtgg gtatttgtaa gatttcgcag
     3361 gtcagcgaag cgtccagata ccccgtggcg cctatttgaa ttttcggatt gcggctttcg
     3421 acgatcaaag gcggcagcca gccgatctcg agctgccgtc ccacgacaat gagcaggtct
     3481 gccctctgca agctgagcag aaagctaggt ttgggctcga cgaagtgcgg atcctggtag
     3541 cccttggcga tcgagactac gttcacccgg tccccgccga cctcgcgagc cagcgccgcc
     3601 atgtcctccg tcgagccgac gacgttgagc ttggaagcgg ctcggccggt actcaggcag
     3661 cctgccagta gtgcgattgc cagagctctc aggcctgacg gcagcttcgt aatcatcgcc
     3721 caggaaacct gcaatctagt agacggctac gggagggcgt ccggttcatc gaaaatcggc
     3781 cggcagccga attctcctgg cgtgcacaat cacccgggca agcagcccag ggcgaggtca
     3841 ctccacgggt gagttgccgg ccacggtcgc cacgtttgct tccatttcgt gagcggcgcc
     3901 gaagagcttt ctgaccaaac cgcgaaccga agccataaga atcaaaacaa tgccgaatgt
     3961 gcaaaccatg gtagctccgg tgggaaggtc gatttccacc gataggatgc agccgagggc
     4021 ggaaaccagc gttcccatgg tccacccaat ggccaggcgc cggccgatgc gctcggcgta
     4081 aaggatagcg gctacagaag gaacgatcag gtagcagaag accagcagga cgccggcaat
     4141 cgacacggat gaggttacga caaagccgaa cgatgcataa aacagaaaat cccaaaagcg
     4201 gaccgacatt ccctgcgcct ccgcttctct gggatgcatg gaaatcagta agaacttttt
     4261 gcggaagata aaatggaaca atccaatcgc gccatagaga gccgccgttt tttcaacatc
     4321 agtccaggaa accgtcaaaa tgttgccgac gagcatctct ttcagatgct ccgtttcggc
     4381 gatcgatttg ctcatcgcca gaatggcggc cgccgatgcg acggcgtaag atatgccgat
     4441 gatagcttcc tggggtatgc gggcgcggtg cccgcgaatt gtggagaaaa cggcggcgcc
     4501 gatgaaagtg aaagccaggc tcacccagta agcccctggg gagtgcgggt ccatgccgca
     4561 gagaacggct atcgtcgtac ccagcgccgc aatttgagcc aaagccagat cgacgaagat
     4621 gactccccgc tccaccacgt gcacgccgag ataggcgtga attcctgtta atatcaggct
     4681 cgcgacgaaa ggcgccgcta aaaagacgag catatccata acgtgctcct aagtcacctt
     4741 gctgaacagg ccggtaagca gattgatgtc gtaatcaaac aggtcgaaat aggtcttgat
     4801 ttccttcacg ccgcctaccg aaggcatcaa ctgaagcgcc tgcgctcctg tctggcgggc
     4861 gattgattcc ggcgtccgcg agtcaaaata gggctcgacg agaatcgctt tgaccttttg
     4921 ctccttcatg aaagcgatca gctcgagcgt gtgactgggc gtcggcggga tgccgggctt
     4981 cggttcgaca tagccgacga ccttcagccc aaaccgcttg gcgaagttcg gccacgagtt
     5041 gtgataggtg acgacttgtc gtccccggaa gggagccatc ttcgcatccc accgtttttc
     5101 cgcttccgcc agccgcctgt caaaatcccc atagcgctgg gcgaaatagg cggcatcggc
     5161 cggatcgagc tgcgaaagtt tctcctggat cgccttggca atgcgccggc cattttccgg
     5221 gtcgagccag tagtgaggat tccccactgg gtggacatcg cccatcgcgc gggtcacttc
     5281 gacggtgggt atctccagga tgtcacaggt cagcgaagca tccaggtagc ccggcgcccc
     5341 gatctgaatt ttcggattgc ggctttgagt cataagcgcc gggagccatc caatctcgag
     5401 ctggcgcccg accgccacaa gcagatcggc cttttgcagc ctcagcaaaa agctcggctt
     5461 gggctccacg aagtgtggat cctgatagcc gcgcgcaatg gcatccacat tgacatggtc
     5521 gccgccgacc tcgcgggcca gcgccgccat gtcttccgtc gtgccgacca cgttcagttt
     5581 ggcggcggcg gccggagccg ccgctgcggc ggccaaaaaa gctatcagag cgctgccgaa
     5641 aaaataacgt cgtaacaggg tcatggaatt tagcctcatg cgatgcattc cttgccgtcc
     5701 ctaaaacgga tgcgcgccgt gggcgccgat ggaaaacatg aactggaaca gcagttcatt
     5761 agccgtggcc tcgttgagat aacgcgtgcg ccggagctgt gcgcgaacct ggctgaattc
     5821 gctcggccgg taggtgagca gcacggaccc tccggtgtcg tgcacagccg agtcggtgag
     5881 tctgtcggac cggtcaaaac ggccgccaag aaaccagcgc tggcggaatt gatattctcc
     5941 ggaaacatag tatccgaatg gccgggaaac cccaaccggg agagccgccc ggccccaaac
     6001 catctcggtg cgcgcgatga aggaccggta aatggcccgg cgcagcggct tccaccgcag
     6061 ggtggcatcc aatccgtata actggttgac cagcgcttcc cccagcgggc tgtggccgcg
     6121 cgcataggaa acgcccaaat ccagatttgt ggattcagtg atgtcgcgat aggcccgcag
     6181 gtggtccacc gtacttatgt tcgacttacg gtaggactga aaaacatcgc tggaatcccc
     6241 tcggtaaacc tgacctgtgg cctcgaggaa gacctgtccc ggcgcgggaa tagtgcgcga
     6301 taacgaaagg cccgcatcgc tgattccgtc ctccccgccc agcagatttt gcgtcactaa
     6361 aggccggtca atccacggga gcacgtgcaa atgcatcgtg ttcacctttc cgaacgccga
     6421 tcgcatcttg ccggccctca attggagagc gcccggcaga gcggtgaagg tcatatagcc
     6481 ctcttccagg tccaccccat gctcgctgaa gcttaagaag acatcggccc gggcatacgg
     6541 gtccacgatg gcctgaaaag ccagctcgct ctcctgcatc tcgaacgccg gcacggggtc
     6601 gagcgagagc cgcgaagcgc cgtagccggc ggagccgcga aaggttccaa tggcagcgat
     6661 ctccgggtta aaaatcttgt ttcccagcgt ttgggcgcca tagtagggca ggccgccggg
     6721 accggttacc ggcgcttgaa cggctgcggg cgcctgcggg ggcgcagcct cgggagcggg
     6781 ctgttgcggg gcccccggtg cagccgcggc cgctggaggc tggctctgaa gagctcttac
     6841 ttcggcttcc aaggcttgaa tcctcgcttc cattgccttg atgcgggcca gaagttcggc
     6901 tgtgtcctgc gccatgagga tgctggccga agcgatccag agtccgaaag gtaccagaat
     6961 ttttcgtatc atgttctccc aagtccccct aagttgcgct aaatcaagaa cgaagccagt
     7021 cagcgaaacc ggcggcagtc aacgatgacc ttcgggcggc gctgcggcac gcaccctacg
     7081 gcggttgatt ttcacgcagg aactagacgg ggggagcgcg gggagatctg gaaatcaggg
     7141 ccggttccgg tacggcgtgg agcttcacgc tgggcgtccg ccactcaacg gcggccggcg
     7201 cctgaatttc cactcgcgtt agcggctggg ggcttggcaa atggcccgtg cggcagatat
     7261 cgcagcttcg cccctgcgga ccgttatgcc agtgcccggg gagcacggtg gcgttcgcga
     7321 tggccagcat cgccaccagc gccaccaggg cgaccgcttt gtgcagcacc ttgctatgcg
     7381 gggcaggcat gagtaagcac gcagcctggg cacattttta cagatccgga gcctcaagat
     7441 caagaacaac aagaactaat tctttctgtg cgggcttttg gccggccgga agcgcaatgc
     7501 cgatcctgcc gtctggcccg gtttgagcga gagctttcct tccctgaggc gcgccgtggg
     7561 aagcaggcgg cctgcgctct tgcttgagcg ctttcataac ccggagtgcg gcaacgcgcc
     7621 tcgacggttc cgagttttcg gcgagcccgg gaagcactgg caacgctgct cagttatcgg
     7681 ggttccggtc aggcggtctc ctcggaagct ccgcccgcgg catgacagac gtagatttcc
     7741 ggttccatgc caaagtgctc tcggtaacgt gcggctatcc ggcggcggaa ctcagggacc
     7801 agcctggtac ggaccaggtt cacggtcgat ccgccgaagc cccctcccgt catgcgggcg
     7861 ccgatcacgc cgtctatctt gcgcgcggct tccaccaaga aatccagttc gcggcaactt
     7921 accccgtagt cgtcccgaag gctgttgtgg gattcgtaca tcagttcgcc ggcgcgtatc
     7981 agatcatttc tctccagagc agcgacgaag cgatggaggc gcgcgttttc gctgatcaca
     8041 tgccgcgcgc gcccgcgcca tggctcggga agccgttcgg cggccgccag attcgcctcg
     8101 cgcagtgaag agcggagcat ggcggcagcc tggtcgcatt cctgccgccg gcggttgtat
     8161 tcgctggaag cgagctcatg gcggaccatg gtattcacca ttaccagcgc gcagtcttgc
     8221 ggaaggggca cgagccgata gtgcaggctg cggcagtcga ggagcagagc gtgatctttg
     8281 cagccgagca gggagacaaa ctggtccatg atgccgcatc gcacaccgac aaactcgctc
     8341 tcagctcgct ggcaggcccg ggccagatcg acccgcggga tgtccttgcc ggcaagggcg
     8401 cacagcgcca gggcggcggc cgcctccagc gcagccgagg agctcagccc cgctcccggc
     8461 ggcacggaac tgtcaatttc aagcatcgcc ggcacggggt tcacaccgag gccccgcagc
     8521 gcaactgcca cgccggctat gtagtcgccc cagttgccgc gccgctccaa ccgatcaatc
     8581 ggccagctaa cctcagcttt gagattgacc gaacgggccg tgaggcggtt ggcatcgtgg
     8641 ggaaaaacag taatgcggca agccagattc aaagccgccg gaagaacgaa tccctggttg
     8701 taatcggtat gctcgccgat aaggttcacc cggccgggag cgcaaaagat tctcattttt
     8761 tagctcgccg ggcgagcgcc gccatggtga ttccgaacga aagcataaaa aggccggcat
     8821 ctacattcaa attgatgggc gtgagcggag cgcgcaggtc gggatgcaga agcccgtagc
     8881 ccaccaggat ggcgccgagc agaaaaaaca tcagcccgat gggataacga aggtcaaggc
     8941 ccatggcgcc acctaccaga aataccagtt cagcgcgatc aggatcgcgg ctaccaccag
     9001 cgccagggtc gccgggcgct cataccagca aaggccggcg gctttagggc gctcggtaag
     9061 gctgtaaaca agtccccgca tttcttcgtc cgccttcggc cgggtgagca ggctgaccac
     9121 aatggttacg acaaagcagg tggtccaagc gacgatggcg atccagaagt tctgcgccat
     9181 ggtggagggg agcgcgcgcg catgggcgat ccacgctccc ttgccctccg ccgaggtgag
     9241 accatgggtg agcgccgccg ccagcgtgcc tgccagcagg ccggaaaacg ctccgtgacc
     9301 ggtagccctc ttccagaaca tgcccagcaa aaaggtcgca aacagggggg cgtttacgaa
     9361 tccgaagacc agttgcagca ggtccatgat gttgttgtag atcgtcgcga cgtaggccgc
     9421 ggcgatggat atcaataccc ctgccaccgt ggcaatgcgg cctacgcgga gatagtgcgt
     9481 atcatcggct ccgctccgga tgtgcgcttc atagatatca aacgtccaga cagtattgaa
     9541 agccgtaaca ttaccggcca ttcccgacat gaaggaagcc atcagcgcgg tgatgcccac
     9601 ccccaggatt ccggaaggat agaactgcgc gagcaaggtg ggaaacgcct ggtcgtaatc
     9661 gtagactccg gaagctttca tgggcagcgc gtagccgctg ccgcccatcc gtgtgagggc
     9721 cacagcaacc aaccccggca ggatcactat gaacgggatg aacatcttgg ggaaggccgc
     9781 gaccagaggg gtccgccgcg cagacgccat cgactcggaa gccatcgcgc gctgcacgag
     9841 caggaaatcg gtgcaccagt aaccgaacga gagcacaaac cccagccccg caaccattgt
     9901 aaaggcatcc actcccatgg gattttcggc ggcgctcccc atgtatcgcc acgaactagt
     9961 cattacggcg ggaagttttg agcgcaggcc gtcccatccc cccaccttcg cgatgcaaac
    10021 tattgccagc ggcgacaggc cgatggcgat caggaaaaat tgcaaaacct cgttgtaaat
    10081 cgaagcggtc agcccgccaa gaaagatgta aacgagcacg atgccggccg agagcattac
    10141 gcaagccgtg aagctccaac ccaggagcag ttggaacagg cggccgagtg catacatgga
    10201 gatgcccgaa gagaagaccg tcatcaccgc gaatgtcacg gcattcaatg tgcgggtctt
    10261 ctcgtcgaag cgcagcttta gatact
//
LOCUS       DASREK010000268        21594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1607868, whole genome shotgun sequence.
ACCESSION   DASREK010000268 DASREK010000000
VERSION     DASREK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 21594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21594
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607868"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1241
                     /locus_tag="VFA54_14550"
     CDS             228..1241
                     /locus_tag="VFA54_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52083.1"
                     /translation="MRGSVVLCTMAAAGFLTVPALLGAAAQPIGFAVADSKFTVGSSV
                     VDGLVTLFEGDTITSSYLATRLNLRDGSRYVLGIDSEGIVHRDYLSLESGSVEIVGQS
                     GAPRVVAAPLSVTAGKPGTAATIYRTGKSRISVQVRAGEVKVALSGSSQVSTLGAGQL
                     VSIRVAPKGGMQVDSDGAVFDVTQIQSQQLASLLEASKSYTCLQPRATGAAHSFAALS
                     SQLAAARATQNVIAGRIASGAATALDYQSMASVNNTVKNLNQASASLSQELDVAVDIT
                     SEFHHPGPPAPYPSPHTVHGHINLYPHHGQHGHTLPPDYPGNTPYGDHQVPPHHYAQP
                     PAG"
     gene            1436..2656
                     /locus_tag="VFA54_14555"
     CDS             1436..2656
                     /locus_tag="VFA54_14555"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZS52084.1"
                     /translation="MTGSATSRVRLLGARLVLLTLSLAFAGAIAEGLLRWVFHAAPLL
                     DVDIYYLDSQRNLRMLPGVRRRHVTRLWDVDITINQEGFRDRISPIASPAPPVLALGD
                     SFAFGWGVNLEQTYLYLLEQRLNALRPIRVVKAGTPGTGTGDQFHLFQEICGAYRPQM
                     VLLSFFVGNDFTDVQMGGINQFDVKDGLMIRRELRPSSWTQAVREKAVRSSHLLQFLR
                     AVQLDFERRRAAASLAANPALAAHDPWLFEFSKVHLRRFPEETSRGVAETLRYLDRFD
                     DFCRERGMDFVLMVLPRSIQVNREEAAEWQSAFHISDQDLDLDHPQAVLRTWAQRRGV
                     RMLDLLPGFRSYHAVHPEIKLYYYPDAHFSPAGHRLAAEILASYLAKEGLPRLPAAAQ
                     ETSRHGSGSPASTF"
     gene            2789..3724
                     /locus_tag="VFA54_14560"
     CDS             2789..3724
                     /locus_tag="VFA54_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS52085.1"
                     /translation="MADLRREGVRRCVLAAAFIVLLLAALERGGFGQSHTHAVRRLAP
                     DVYFWQGDHVIHEPANVGWVIFRDYVFVIDANFPWGAREILPEIRKTTNKPIRFLFNT
                     HYHADHSYGNGVFTEAGAAVVCTADCLEEARSKGPSDTKNQARGRADFPMVNASVVFP
                     DTLAFDDGQRRVELIRMGPAHTIGDAVAYLPKEKIAFIGDLAVNWTWGNNFGDPDADY
                     ANWLRALDKLAQWDLKTVVPAHGSVGTADILRGQRAYLNDMWSQVRRGLQAGKTPGQL
                     VQEIDLSRHKPFGERPESNAAGIRAMCRNAPKQKS"
     gene            3947..4927
                     /gene="galE"
                     /locus_tag="VFA54_14565"
     CDS             3947..4927
                     /gene="galE"
                     /locus_tag="VFA54_14565"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZS52086.1"
                     /translation="MAAGSVLVTGGAGYIGSVAAERLLERGEPVVVLDNLSRGFEDAV
                     PRGARFIEGDIADRGLLRSLFREHSVRAVMHFAAFALVGESVQTPEIYYRNNVIGSLT
                     LIEEARAAGVERFILSSTCAVYGDRNTGPLAETMPRAPVNPYGETKMAIERALEWIHA
                     ASGWPYFALRYFNACGATAGHGERHNPETHLIPHVLRAAAGEAESVSILGADYPTPDG
                     TCIRDYIHVEDLADAHICALHAPAETSGAYNVATGRGYSVREVIEAARRLTGRPIREK
                     ICPRRPGDPPMLVADASKIQKTFGWRARHDLDSALRSAWAFQMRQQAIRS"
     gene            4931..5899
                     /locus_tag="VFA54_14570"
     CDS             4931..5899
                     /locus_tag="VFA54_14570"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS52087.1"
                     /translation="MQSALQSLPFRLICPSCLAPLEADGEALLCSRCRRSVPIRDGVP
                     SFCGSDPFYEEYTSHHVPYHLSPAGLKGVVLRFLPYWSWREWRFWRRYVPRGGWLLDL
                     GSARGRQVFAERAAGCVGVDTAFTALVECARYYRLAVQSNLKPLPFDTGSFDCVVTSH
                     VIGHIPLPEKDALFREIARVLKPGGRSVNVIETDSLHPLIGFAKQRPDLYRRYFIDPD
                     GHVGLELPSAVIERFRRHGLHAAACWKMDAGPFHPRLWLKHFDNEYCDLSPEIKRAVE
                     RSRRILQSPARLAAAEVALGLVHYTAGQWFYPLDHSQFIAAVFEKR"
     gene            6002..7123
                     /locus_tag="VFA54_14575"
     CDS             6002..7123
                     /locus_tag="VFA54_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZS52088.1"
                     /translation="MQLAVIARRLCGPLTGVGRYLEYLLYYWSRTDSPFRRILVYAPS
                     EPSLETAAIAGPVQLRVLPSRLPPLVWENAVLPLRMEAAGVVFGPYTVPWQCRGVKVV
                     SNLGIYESRPEDFSWWQRARTIPFFRHSARTAKLTIANSESTRRDLVRYLGVDDSKIR
                     VIYPGVDDAFHPAGGEEIPLRISITYRLPARPFFLFVGKLSLRRNVPMLLDAFARLRR
                     QKSLPHVLLIAGPDHLGVNAPRLIEERALQDAAFYLPFVPRADLVELYSAATAFVLPT
                     LHEGFSFTILEAMACGAPVITFDHPPLREAGVGEAAIAIEEPSADALFDSMRELIENP
                     SRREQLRRAGLACASRFSWKQVAADTMKVLCEAAGLAVR"
     gene            7229..9535
                     /locus_tag="VFA54_14580"
     CDS             7229..9535
                     /locus_tag="VFA54_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS52089.1"
                     /translation="MIRRLFLLLVSFALWGQTNTGELRLMITDSTGLPIAGSVELVSE
                     VNQYRQTFQADSQGQVTAKLLPYGLYRVQVSREGFAPFSSLVEIRSAIPKQLKIVLGV
                     APVETTVTVSEDETLIDPHRTGPVNRIGAETLRDRETALPGRSLLELVNTQPGWLLEA
                     NGVLHPRGSEYQTQYVIDGIPLTDNRSPAFAADPDADDVQSMNVLTADYPAEYGRKLG
                     GVIEIVTARDKRPGFHGKVVGSGGSFDTAGGYAMGQYGWGRNTVSLSADTARTGRFLD
                     PPVEQNFTNRGTTSGVMAHFERDITDRDRAGVILRREESRFLVPNELIQQTAGQRQDR
                     DSYETSGQVSYQHIFSPDLLGDLRGMARDLSAGLWSNPLSTPIIAGQGRSFREGYVKG
                     ALSLHHGVHEFKAGVEADFASIQERFQYQITDRDQFDPDTPPAFAFFGRAQDREQAFF
                     VQDLVRMGNWTLSAGLRWDHYRLLVDENAWSPRLGASWYWPAADLVFRASYDRVFQTP
                     AFENLLLASSDAITALSEEVLRLPVRPSRGNFYQAGFAKGVLRKLRLEGTYYHRDFTN
                     FADDDLLLNTGVSFPIAFRKADIHGTEVKVEIPRWGPVSGFISYSNMRGVGYLPVTGG
                     LFLGDEASEAIRSTSSFPVTQDQRNTVNSRLRYQVASRFWVAAAGAYGSGLPTEFSGT
                     EAEAAAQYGQRIVDRVNFTRGRVRPSFALDASAGAIVRKADRGNLRLQADIRNLTNRL
                     NVIDFAGLFSGTALAAPRSYALRLQMDF"
     gene            9685..10425
                     /locus_tag="VFA54_14585"
     CDS             9685..10425
                     /locus_tag="VFA54_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HZS52090.1"
                     /translation="MSHTPISASDLELLRRYDTPTICNVIEVFNVRPRNTGYCDSRIR
                     ACFPEMPPMVGYACTATFRSAAPPLKGDVYASLDRHVASFSEVPFPPAVVFQDLDDPA
                     QAATFGEVMCTTYKRFGAVGLITSGAARDLDQVRALNFPCFSNGVICSHGYAHFVDVN
                     VPVRVGGVVIHPGDLLHGDANGVTTIPHEIASEVAHACAEYVAAEQIVLDYCKSAEVS
                     AAGLAEARAECGRRIAALSEQVRAGAAR"
     gene            10708..13266
                     /locus_tag="VFA54_14590"
     CDS             10708..13266
                     /locus_tag="VFA54_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS52091.1"
                     /translation="MRRPLRLLLAALVLASLARAQSGGVAVAGVVQDPSSAPIPAASV
                     VLAMPGSSQLQTTSTNASGAFRFDRVPPGEYQVRVQHDGFNLATASLRVGARPPSPLK
                     ITLTLATVRQEVTVSEETTQVNTDTSQNQDAVSMDRQSLDDVPIFDQDYVGTMSQFLD
                     SNLVGTNGATLVVDGLEATSAGVTASAIQEVRINQNPYSAEFPRPGRGRIEIITKPGA
                     PQYHGAFNFLFRDNYLNARNAFAAVRPAEQRRIFEGYLTGPAGHSQMTSFLISAHRQE
                     EDSQSIVFAQGVGGAIQQNVPSPSRNTQIAGRINHNYTGGSSISLLYDFKDRTSNNQN
                     VGGLTLPEAGVNTQFREDELRWTYKAVVTPVLLNDFTVMFGRAHAPTFSIHSGPQIVV
                     QGAFTGGGAQADQLRTENHVNLSEIASWTHGRHYVRAGFQIPDFSRRGLDDNTNRAGT
                     FYFSTLQDYMLNRPYSFVQQQGDGRVVFTEHVFGGFVQDEIRLLPNLSVSAGLRYDWQ
                     NYFRDNDNFAPRISFAYSPDRRRKTVFRGGAGVFYDRTGPGPIFDLLRYDGTRLRRYV
                     ITNPGFPDPLSSGGPLAAQPTSIVRLAPDIRIPYTLQYSFAVERQLQRRTTLTVTYFG
                     TRGVSLFRSRDLNAPPPPLYAARPDPDFSVIRQIESSGRSETHSLEVSLRGNVSRYFN
                     GMIQYTYGRAYNNTSGINYFPANNYDLSGEWSRADWDQRHRLNLFGTIKATKLFNLGV
                     ALWMHSGAPYTMTTGFDDNHDGVATDRPPGVPRNSLQGPFYADFDLRWWHDFLLEKSK
                     KEKGPVATIGLDAFNVINRVNYTNFVGDISSPFFGRAVSAQPPRRLQASFRLRF"
     gene            complement(13291..14025)
                     /gene="truA"
                     /locus_tag="VFA54_14595"
     CDS             complement(13291..14025)
                     /gene="truA"
                     /locus_tag="VFA54_14595"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZS52092.1"
                     /translation="MRNLKLTLAYDGTDFHGWQIQPDLPTIQGELERVLREIEGKPVA
                     VAGSGRTDAGVHALEQVASVKLQNPIPTTNLRRAMNRLLPPSIRILQVEEAGPAFHAR
                     FSARSKVYEYRLWRGEVCHPLMYRYVYHHPYPLRVADMVQAAPLFEGTHDFRSFAAAD
                     PAEPGAVRTIFSSRLEPEGDLLRYTVCGSGFLHHMVRNIVGTLLEIGKGNLTPGDVPA
                     ILAAADRSRAGPTAAARGLFLVKVEY"
     gene            complement(14115..14717)
                     /gene="pyrE"
                     /locus_tag="VFA54_14600"
     CDS             complement(14115..14717)
                     /gene="pyrE"
                     /locus_tag="VFA54_14600"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZS52093.1"
                     /translation="MAAERIAQAEVLELCRHTGAYLQGHFRLTSGLHSPEYLQCALLL
                     ACPSTAGRLGAALAQALGPLLAGRPADFVIAPALGGLIIGHEVARALGCRFLFTERDS
                     DGRMALRRGFAVEPGARGVAIEDVITTGGSTAETIAAVQQAGGTVVAAGSIIDRSGGR
                     ADVGVPRVALATLTVQTYAPDACPLCAQGLPVIKPGSRKG"
     gene            14925..16967
                     /locus_tag="VFA54_14605"
     CDS             14925..16967
                     /locus_tag="VFA54_14605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS52094.1"
                     /translation="MRLKAVFASLLSVLMVQPVWGQGAPSPAPAEPAVAQSQKISQPD
                     YTKGNIWWPNFTSVFRTPYVAEVNMANSNRLEQLLRDGNLYLSLEDAIALALENNLDI
                     AYTRYQGPIADTDILRSKAGVPIRGLVNPGQISSFSTGNSATGANGVNPIGSTTNAST
                     ISTFATGSTITGPNGLSVVGTNILGGAPLPQLDPVLTTTVGWAHNSTPQSNTVITGTN
                     TLISQGASTQIAYSQAFLTGTSFSLSFNSATSNSNAIRNTLNPALNSSATLNFTQPLL
                     QGFGRSMNARYLRIARNNREVADLLFKQQVIQTVSQVQNLYWDLVGFNENVRVVQQAL
                     QLAEKLYADNKRQVEIGTLAPIEIVRAEAEVAARQQDLTLAQTQVQQQEVILLNALSK
                     NGVASPSVAQAHIIPTTRLTVPPVEPIEPVQDLISMALQSRPELAQSRIGLTNSDIAL
                     KGTRSAMLPQLNLVGTLTNNALAGEVNEHFVAIGGGSAPPSPFFVGGFGTALSQIFSR
                     NFPTYSFGVQLNVPIRNRAAQADMAAAQLQHRQAEIRLRQAENEVRLEVKNALVGLQQ
                     ARARMDAATKSRILEEQTLDAEQKKYALGASTIFNVIQVQRDLASAREIEVAAINNYA
                     KARVELDRATGQTLAKNNISMEEAFEGNVSRPPDPIPANPPAPVAAPAPPSPAPAP"
     gene            complement(17095..18447)
                     /locus_tag="VFA54_14610"
     CDS             complement(17095..18447)
                     /locus_tag="VFA54_14610"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HZS52095.1"
                     /translation="MKPLRLLIAVVILGGLGGAVWYSERHPPSPETKTNTASQKILTV
                     PQDQVKRLQFTRPGGSGDEFTLEKGDDGKWKVTEPKVYKADETALNTMLSNLTSLTAD
                     QVLSENNTDWATYGLDPPKLVVTMTLKDGKQNKVSFGLDAPASSVVYARLNQDGKLFT
                     VPSYVKASLDERLVDVRDRHLMPFDDSKVARVVLTANGQTLEFSKAGSDWQILKPRLM
                     RADTFAVDDMVRSAHNLIFDSVIDESGKPPAKYSFSSPYEVLEVTDASGTYALTVGRE
                     RQKDSTTYTYYAKTTAMPGVFQISSTVPDALNKNLDSLRNRKLFDFGFNDPAKVEVRD
                     GSSQMVIEKKNGKWLRTDAGSKELPADKVQTLIDHLRNLSATSFPSDEASAQSRYGLN
                     KPTLEAKVTSDDGKRVEHVLMAAGPESKYYAARENEPTTYEIDKTAYEDIQKSIGALK
                     "
     gene            complement(18454..19848)
                     /locus_tag="VFA54_14615"
     CDS             complement(18454..19848)
                     /locus_tag="VFA54_14615"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HZS52096.1"
                     /translation="MKMGWISARQTKYGAYVAVYLVVVLAILFAANFLANRYDKTYDA
                     TSNKVFSLSDQTKKILANLKQNVTIYYFNTSNQTNESAFGPSPRDLLQRYSSASHYVT
                     VDFVDPVKNPKRALDMKVTQTNTTIVEVNGRREEAKSPTEEQVTNALIRALKGGKRTA
                     CFVTGHGEADISSSEPNGFSGAKDALEATNFTTKTISLLEKNAAVPSDCTVLVEAGPK
                     NNLLDVEIAAIKKYVEGGGRALFLIDPETKGVNTSALDKMIGDWGVKVDNNIVVDLSG
                     VGQLFGADELSPLVTSYESQPITQDMKNVACLFPLSRSVEPDPDSRNSNVLREKLFQT
                     TSRSFATSNFASGQIKLDPKRDKTGPFSLAVAGTYRTDKENVQGRFVVVGSSRFAVNA
                     TLRFPGGNRDLFLNMMNWLSSDEDLISIRPKESEDRRLSLTVSQMARIFYASVIGFPL
                     LIIVGGTYVWWRRR"
     gene            complement(19853..20620)
                     /locus_tag="VFA54_14620"
     CDS             complement(19853..20620)
                     /locus_tag="VFA54_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS52097.1"
                     /translation="MKNVWVLAKRELNSYFASPIAYIVLAMFAVIFGYFFYSATALFV
                     DYSARSMMQPGGPPMNMNDFIIRPLLMNVSVIVLFLMPMITMRLFAEEKRSGTIELLL
                     TSPLRDTEIILGKWLAAMLLYLCLIGISALNILFLFRYGQPDWRALLVGYLGLILLGA
                     AFLGLGSFLSTLTKNQIVAASLTFGVFLLLWVLDWVSSFSTGTGGKLASYLSITTHFE
                     QFAKGVLDLKDVVYYLSVSFLGIFLTARSMESLRWRA"
     gene            complement(20617..>21594)
                     /locus_tag="VFA54_14625"
     CDS             complement(20617..>21594)
                     /locus_tag="VFA54_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS52098.1"
                     /translation="KVQGLTKRYGRATAVDHISFEVEKGQIVGFLGPNGAGKTTTMRV
                     LTCFLPPTEGKASVAGYDVLEQPLEVKRHIGYLPETPPLYPEMEVDEYLVFAGRIKGV
                     PASNIKRRVNEVVERCALGEVRSKLISKLSKGYRQRVGLAQALIHNPDVLILDEPTAG
                     LDPKQIIETRKLIHDLSGEHTIILSTHILPEVSSTCQLVIIINQGKLEAIDTPENLTA
                     RLQGHETIFLDVEGPADQVASRLSSLQGVNRVVRKDGQAGRCSWEIETAKDHNIRSEL
                     ARAVVTAGWGLHELRPVGLSLEEIFLKLTTADVAAEPAAAERRASGGAA"
BASE COUNT         4065 a         6566 c         6604 g         4359 t
ORIGIN      
        1 ggaacctcga tccgtgctgt accggcgtac caacgtctgc atcggcactg gcggcaggcc
       61 ggcgggcaag tcaaaataac cgggttcgtc tgccatacct ccccttagta acaggagttt
      121 ttcgcctgat cttgtaactt agtacttgac accccttcgc cgtaatccca taataactct
      181 ggcattagta aaggctgggt acgtcggaac tacagggaga atggcgaatg aggggaagcg
      241 tggtgttgtg tactatggct gccgcagggt ttttgacggt ccccgcgttg ctgggagccg
      301 ctgctcagcc tatcggtttt gctgtcgcag attccaagtt tacagtggga tcttcagtcg
      361 tggacggctt ggtaaccctt ttcgaggggg ataccattac tagttcctat ctggctaccc
      421 gcctgaactt gagggacgga tcccggtacg ttttggggat cgactccgaa ggtatcgtgc
      481 atagggatta cctgtctctg gagtcgggaa gcgtcgaaat tgtgggccag tcaggtgcgc
      541 cgcgcgttgt cgccgcgcct ttgtcggtta cggcgggcaa gcctggtact gccgccacga
      601 tttaccgcac tggcaagagc cgaatcagcg tgcaggtgcg cgccggcgaa gttaaagtgg
      661 ccctctcagg aagctcgcag gtgagcacgc ttggcgccgg gcaactggtc tcgatccgcg
      721 ttgcgcctaa gggcggaatg caagtggatt ccgatggcgc cgtctttgac gtaacgcaaa
      781 tccagtcgca gcaacttgcc agcttgctcg aagcctcgaa gagctatacc tgcttacagc
      841 ccagggcaac cggcgcggcg cactcgtttg cggctttgtc ctcgcaactc gcggcggcac
      901 gggcgacgca aaatgtgata gccggccgga ttgcaagcgg ggcggcaaca gcgctcgatt
      961 atcagagtat ggcttcggtc aacaataccg ttaagaacct gaatcaagcc agcgcgtctc
     1021 tttcccagga gctggatgta gcagtggata tcaccagcga gtttcatcac cctggaccgc
     1081 cggcgcctta tcccagccct catacggtgc atggacacat caacctctat ccgcaccacg
     1141 ggcagcacgg tcacactttg ccgcccgatt acccgggcaa taccccgtat ggggatcatc
     1201 aggtgccgcc gcaccactac gcacagccgc ctgccggatg agcaagagcg gcgggtcgag
     1261 tcgcagcgga gttagcgctt ccggccgcgc tggatcgccc ttggcaaggc tctgaggcga
     1321 gccacggcgg cggatttccg ctggacagac cgaatcactg gtttcagaat tctgttggaa
     1381 acggaaaggc cacgctctct aaggcgtggc tttctttttg ctataacgaa gcagaatgac
     1441 cggctcagcc acctcccgcg tccggctcct gggggcgcgg cttgttctcc ttaccctcag
     1501 cctggcgttt gccggagcca tcgccgaggg gctgctgcga tgggtattcc atgccgcgcc
     1561 gttgctggat gtggacatct attacctgga ctcccagcgc aacctgcgca tgctgcccgg
     1621 cgtgcgccgc cggcacgtca cgcggctgtg ggatgtcgat atcaccatca accaggaagg
     1681 ttttcgcgac aggatttcac cgatcgcttc gccggcgccg cccgtcctcg ccttgggcga
     1741 ctcgttcgcg ttcggctggg gggtgaatct ggaacagacc tatttgtatt tgctggagca
     1801 gcgcttgaac gcgctccggc cgattcgcgt cgtcaaggcg ggaacgcccg ggaccgggac
     1861 cggcgatcaa tttcatctct tccaggaaat ttgcggcgcc taccgcccgc agatggtatt
     1921 gctcagcttt ttcgtaggga atgactttac cgatgtccag atgggcggga ttaaccagtt
     1981 tgacgtgaag gacggcttaa tgattcgccg ggagctgagg ccctcttcct ggactcaggc
     2041 ggtccgcgaa aaggcggtgc gctcctccca tctgctgcaa tttctgcggg ccgtgcagct
     2101 tgacttcgag cggcggcggg cggcggcgtc gctggcggcc aacccagccc tcgccgcgca
     2161 cgacccctgg ctattcgagt tttcgaaggt tcatttgcgc cggtttccag aggagaccag
     2221 ccggggcgtt gcggaaactc tgcgctatct ggaccgcttc gatgattttt gccgggagcg
     2281 cgggatggat tttgttttaa tggtgctccc gcgcagcata caggtgaatc gggaagaggc
     2341 ggcggagtgg cagtccgctt ttcacatttc cgatcaggac ttggatctgg accacccgca
     2401 ggcggtgctg cgcacatggg cccagcggcg gggagtccgg atgctggatt tgctgccggg
     2461 tttccgcagc taccacgcgg tccatccgga aatcaagctc tactattacc cggacgccca
     2521 cttcagccca gccggtcaca gactggcggc ggaaatcctc gcgtcctatc tggcgaagga
     2581 ggggttgccg agactgcctg ccgccgcgca ggaaacgtca cgtcacggca gcggctctcc
     2641 ggccagcacg ttttgactga tggctggggc gcatcgggtg agcaatagaa tccacatttg
     2701 agaaaatttc ccgaagaagc ggttttcgcc gcgaggaaga ctgggtatct ttgaggcgaa
     2761 gccggctgga ggcgaaggaa cgatcggcat ggcagacctg cggcgtgaag gagtccggcg
     2821 ctgcgtcctg gcggcggctt ttatcgtcct gctgctggcg gccctcgaac gcggcggatt
     2881 cgggcaaagc cacacccacg cagttcggcg gctggccccg gatgtgtatt tctggcaggg
     2941 agaccacgtg attcacgaac ccgccaacgt gggctgggtc atattccggg attacgtctt
     3001 tgtgatcgac gccaacttcc cgtggggagc ccgggaaatc ctgccggaga tccgaaagac
     3061 caccaacaag ccgatccggt ttctgtttaa cacgcactat cacgccgacc actcttacgg
     3121 gaacggcgtc ttcaccgaag ctggggcggc agtggtctgc actgcggact gcctcgagga
     3181 agcgcgcagc aagggcccgt cggatacgaa gaaccaggcg cggggccgcg ccgattttcc
     3241 gatggtgaac gcttctgtag tctttcccga cacgctcgcc tttgatgacg gccagcgtcg
     3301 cgttgagctg atccgcatgg gtcctgctca tacaatcgga gacgccgtcg cctatctgcc
     3361 taaggagaag atcgctttca tcggcgactt ggcggtgaat tggacctggg gcaacaactt
     3421 cggcgatcca gacgccgatt acgcgaactg gttgcgcgct ctcgacaagc tggcgcaatg
     3481 ggatctgaag accgtggtcc cggcgcatgg gtcggtgggt acagccgata tattgcgcgg
     3541 gcagcgcgct tatctgaacg atatgtggag ccaggtccgg cgcggtttgc aggccggcaa
     3601 aacgcccggg caactggtgc aggaaatcga tctttcgcgg cacaagcctt tcggagagcg
     3661 gccggaatcc aatgccgcgg gcatccgggc gatgtgtcga aacgcgccga agcagaaaag
     3721 ttgacgccca cgggcgaagc gcgggtgcgg ggcagcctcg cccgcagatt cccggccctt
     3781 tcttttattt atgagaccca gcccccaggg cggcgagcgc tgaattccac taccgcggct
     3841 cgaatcaggc tggccggagc gccgcttgac gcgctcccat agttctcttg acgcctcggc
     3901 cggccggccg ctaccctgac aacatgatca agtgggaagc cggctgatgg cagccggcag
     3961 cgttttggtg acgggcggcg ccggctatat cggcagcgtc gcggcggagc ggctgctgga
     4021 gcgtggcgag ccggtcgtcg ttctcgataa tctgtctcgc gggttcgaag atgcggtgcc
     4081 gcgcggcgcg cgtttcattg agggcgatat cgcggaccgc ggcctgctgc ggtccctttt
     4141 ccgcgagcat tctgtgcgcg cggtgatgca ttttgccgca ttcgctctgg tcggcgagtc
     4201 cgtgcaaact ccggaaattt actaccgcaa caatgtaatc ggcagcctga cccttatcga
     4261 ggaagcccgc gcggcgggcg tggagcgatt catcctttcc tccacgtgcg cggtttacgg
     4321 ggatcgcaac acagggccgc tggcggagac catgccccgc gccccggtca acccgtatgg
     4381 cgagacgaag atggcgatcg aacgggcgct cgaatggata cacgcagcga gcggctggcc
     4441 ctattttgcg cttcgctact ttaacgcttg cggcgccact gccgggcacg gcgagcggca
     4501 caatccggag acccacctga tcccgcacgt cttgcgcgcc gcagccggtg aagcggagag
     4561 tgtttccatt ctgggcgccg attacccgac gccggacggc acctgtattc gggattatat
     4621 tcatgtagag gatctggccg atgcccacat ttgcgccctc cacgcccccg ctgaaaccag
     4681 cggcgcttac aacgtggcga cgggacgcgg ctattccgtc cgcgaggtga tcgaggcggc
     4741 ccggcgcctt accggacggc cgatacggga aaagatctgc ccgcggcggc ccggcgatcc
     4801 gccgatgctg gtggccgacg cttcgaagat ccagaaaacg ttcggctggc gggcgcggca
     4861 cgacctcgat tccgcgctgc gcagcgcgtg ggcgttccag atgcggcagc aggcgatccg
     4921 atcgtaaaca atgcagtccg ctctccagag tctgccgttc cggctcatct gtccttcctg
     4981 cctggcgccg ctcgaggccg atggcgaggc gctgctctgc agccgctgcc ggcgaagcgt
     5041 tcccatccgc gatggagtgc cgagcttttg cggttcggat ccgttctacg aggaatacac
     5101 cagtcatcat gtgccctatc acctgagccc ggcagggctc aaaggcgtgg tcctgcggtt
     5161 tctcccgtat tggtcttggc gggaatggcg cttctggcgg cgatacgtcc cgcgcggcgg
     5221 atggctgctc gatctcggca gcgcgagagg acgtcaggtg tttgcggaac gggcggcggg
     5281 gtgtgtgggg gtcgatacgg ctttcacggc tctggtcgag tgcgccaggt attaccggct
     5341 ggccgtgcaa tcgaatctca agccgctgcc cttcgataca ggctcgtttg actgcgtcgt
     5401 tacttcgcac gtgatcgggc acatcccgct gccggaaaaa gacgctttgt tccgcgagat
     5461 cgcgcgggtg ctcaagcccg gcggccgctc tgtgaatgta attgagaccg acagcctgca
     5521 ccccctgatc gggtttgcca agcagcgccc tgatctgtac cggcggtatt tcatcgaccc
     5581 cgacggacat gtcggcctgg agcttccctc ggccgtaatc gaacggttcc gcagacacgg
     5641 gcttcacgca gccgcttgtt ggaaaatgga tgccggacct ttccatccgc gcctttggct
     5701 gaagcacttc gataacgaat actgcgacct ctcccccgaa atcaaacgcg cggtcgagcg
     5761 gagccggcgc attctgcaaa gccccgcccg gctggcggcg gccgaagtag cgctgggcct
     5821 ggtccactac acggccggac agtggtttta tccgctggac cactcgcaat tcatagctgc
     5881 ggtgttcgag aagcgctgac gtgcaaagag atccccagat caatcccgtg cgggttgccg
     5941 gaagcgagct gagagaccgc gcggcgcact ccgccttgcg ctccgtcatg cttgaggtgc
     6001 gatgcagcta gccgtcatcg cccgccggct ttgtgggccg ctcaccggcg taggaagata
     6061 tttggaatat cttctgtatt actggagccg caccgattcg ccgttccgcc gcatactcgt
     6121 atacgcccca tcagagccgt cgctggaaac ggcggccatt gcgggtccgg tgcaactgcg
     6181 cgtgctgccg agccgtctgc cgccgctggt ctgggagaac gccgttctgc cgttgcggat
     6241 ggaggcggcc ggcgtagttt tcggccctta tacagtgccg tggcaatgcc ggggagtcaa
     6301 ggtcgtttcc aatctgggca tctatgagtc gcggccggaa gatttttcct ggtggcagcg
     6361 cgcccgtaca atcccctttt tccgccatag cgcgcgaaca gcgaagctca cgatcgcgaa
     6421 ttccgaatcc acccgccgcg atttggtgcg gtatcttggc gtggatgact cgaaaatccg
     6481 cgtgatttat cccggggtcg atgacgcatt tcatcctgct ggcggcgagg aaattccgct
     6541 ccggatttcg atcacttacc ggcttccagc ccgccctttt tttctcttcg tgggcaagct
     6601 ttccctgcgg cgcaatgtgc ccatgctgct tgacgcgttt gcgcggctcc ggcggcaaaa
     6661 aagtctgcct catgtgctgt tgattgccgg gccggatcat ctgggcgtaa acgcaccgcg
     6721 gctgattgag gagcgagcgc ttcaggatgc cgccttctac ctcccgttcg ttcctcgcgc
     6781 ggatttggtt gagctatata gcgcggccac ggccttcgtg ctgcccacgc tgcacgaagg
     6841 cttttcgttc accatcctcg aggcaatggc ctgcggcgct cccgttatca cattcgacca
     6901 tcccccgctg cgcgaagccg gagtagggga ggctgcgatc gccatcgagg agccctccgc
     6961 cgatgcgctg ttcgactcca tgcgggaact gatcgaaaac ccgtcccgcc gcgaacaatt
     7021 gcggcgcgcg gggttggcct gcgcttcccg gtttagttgg aaacaagtgg ctgccgatac
     7081 catgaaggtc ttgtgcgaag cggccggcct ggcggtccgg tgaaggggtc tttttgcaag
     7141 gctctatcgc cgggccggcg atcgcgtttc cggggggtga ggggcgccgg aatgctataa
     7201 tttggccatt gttccttgga gagggcaagt gatccggcgg ttattcctgt tgctcgttag
     7261 cttcgcgcta tggggccaga ccaacacggg cgaactccgc ctgatgatca ccgattcgac
     7321 agggctgccc atagccggtt cggtggaact ggtcagtgaa gtaaaccaat atcgccagac
     7381 ttttcaggcc gattcgcagg ggcaggtcac agcgaagctg ctgccttatg gtctgtaccg
     7441 tgtgcaggtg agccgcgaag gcttcgcgcc gttttcgagc ctggtcgaaa tccgatccgc
     7501 aatccccaag cagttgaaga ttgttctcgg cgtggcgccg gtggaaacga ctgtgacggt
     7561 cagcgaggac gagacgctga tcgatccgca ccgcacgggc ccggtcaacc gaatcggcgc
     7621 cgagaccttg cgggatcgcg aaaccgcgct tcccggacgt tcgctgctgg aactggtgaa
     7681 cacccaaccc ggatggctcc tggaagccaa cggggtgctc caccctcggg gctcggaata
     7741 ccagacccaa tacgtgatag acggcattcc tctcacggac aatcgctcgc cggcttttgc
     7801 cgctgacccc gacgcggacg acgttcagtc catgaacgtt ctgacggccg actatccggc
     7861 cgagtacggc cggaagctcg gcggcgtgat cgagatcgtt acggcaagag acaagcggcc
     7921 cggctttcat ggaaaagtcg tcggttccgg cgggagcttc gatacggccg gcggatacgc
     7981 tatgggccaa tacggctggg gccgcaacac ggtcagcctc agcgctgata ctgcgagaac
     8041 cggccgcttc ctggaccctc cggtcgagca gaatttcacg aatcgcggga ccacatcggg
     8101 ggtgatggcg cattttgagc gcgacatcac cgatcgagac cgcgctggcg tgattttgcg
     8161 gcgcgaggag tccagatttc tcgtgcccaa cgagttgatt cagcagacgg ccgggcagcg
     8221 ccaggaccgc gacagctacg agacgagcgg gcaggtctcg taccagcata ttttctctcc
     8281 ggatctgctc ggggacttgc gcgggatggc gcgcgatctc tcagcgggcc tctggtcaaa
     8341 ccccctgtct acgcccatca ttgccggcca gggccgcagt ttccgggaag gctacgtgaa
     8401 gggcgctctg tcattgcatc acggggtgca tgaattcaag gcgggggttg aggctgattt
     8461 tgcgtccatc caggaaaggt ttcagtatca gatcacggac cgcgatcagt tcgaccccga
     8521 tacgccaccg gcgttcgcat tcttcggtcg cgcgcaggac cgcgagcaag cattttttgt
     8581 gcaggatctg gtgcggatgg gaaactggac gttgagcgcc ggcctgcgct gggatcatta
     8641 ccggctgctc gtggacgaaa acgcctggag tcccaggctg ggggcttcct ggtattggcc
     8701 tgccgccgat ctggttttcc gagcctctta cgaccgggtg tttcagacgc ccgccttcga
     8761 aaaccttctc ctggccagtt ccgatgcgat cacggctctg agcgaagaag tgctgcgctt
     8821 gccggtgcgc ccttcccgcg gaaattttta tcaggccggc ttcgccaaag gggtgctgcg
     8881 aaaactgcgt ctcgaaggca cttactatca tcgcgacttt accaatttcg ccgacgacga
     8941 tctgctgctg aacaccggcg tcagctttcc gatcgccttc cggaaggcag atatccacgg
     9001 aaccgaggtg aaggtcgaaa ttccgcgctg ggggccagta tcgggtttta tcagttactc
     9061 caatatgcgc ggggtcggct accttccggt caccggcggc ttatttctgg gtgacgaggc
     9121 ctccgaggcg atccgttcga ccagcagctt tccggtcacc caggatcagc ggaatacggt
     9181 gaacagccgc ttgcgatacc aagtcgcatc ccggttttgg gtggcagcgg ccggcgcgta
     9241 cggcagcggg ctgcccaccg aattcagcgg caccgaagcg gaggccgctg ctcaatacgg
     9301 ccagcggatc gtggatcgcg tgaatttcac ccgcgggcgg gtgcggccat cctttgccct
     9361 ggacgcgtcc gccggcgcca tagttcgcaa agccgatcgc ggtaatctcc ggctgcaagc
     9421 ggatattcgc aacttgacga accgcctgaa tgtcatcgat tttgctggcc tgttctccgg
     9481 cacagccctg gctgcgccgc gcagctacgc cctccgcctg caaatggatt tctgagcgcc
     9541 gggccgcgga ctgcgatcct ctgccgttgt cttcctctgg catatcgccc gcgcctcccg
     9601 ccgccggatc tgataaactt tttgtccgat gcccggcgcg gcccgcgcgt tcgaggcatt
     9661 cgcaatccga gtgagggtac ttctttgagt catactccga tttcggcgtc cgatcttgag
     9721 ttgcttcgcc gctatgacac cccgacgatc tgcaacgtga tcgaggtatt caacgtccgg
     9781 ccccgcaaca cgggctattg cgactctcgc atccgcgcgt gctttccgga aatgccgccc
     9841 atggtcggct atgcctgcac ggcgacgttt cgctcggccg cgccgccgct caaaggggac
     9901 gtatacgcgt cgctggacag gcacgtagcc agcttctctg aagttccttt tccgccggcg
     9961 gtcgtgttcc aggacctcga cgacccggct caggcggcga ccttcggtga ggtgatgtgc
    10021 actacgtaca agcgcttcgg agccgtgggt ctaatcacca gcggagcggc gcgcgacctg
    10081 gaccaggtcc gggcgctgaa cttcccgtgc ttcagcaacg gggtgatctg ctcgcacgga
    10141 tacgcgcact tcgtcgatgt gaacgtccca gtgcgtgtgg gcggcgtggt gattcatccg
    10201 ggggacttac tgcacggcga cgccaacggc gtgacgacca taccccatga gatcgcctcg
    10261 gaagtcgcgc atgcgtgcgc ggagtacgtc gctgccgaac agattgtact ggactattgc
    10321 aagagcgcgg aggtgagcgc cgccggcctg gccgaagcgc gcgcggaatg cggccggcgg
    10381 atcgccgcac tctcggaaca ggttcgcgct ggcgccgccc gctgagaata ctccgcccgg
    10441 cgatattccg tgccgaggcg aggcttcgga cagggaagga cagcgggccg gccttctcag
    10501 gccaattcat cccctgttca ttttcagcaa gtaaaataaa tgctttggtt aggggctttt
    10561 tcttggagat gttgttcagg aaaagttaag aaagcgtgga gccgcgcctg ttgccgaggg
    10621 catgcgatat aaacaaacaa gcgagcagct tggcgcatta ccgaaggacc ctgcgacgtg
    10681 aactgtggct tgggagctaa gacgcacgtg aggcgcccgc ttcgccttct gctggctgcg
    10741 cttgttcttg ccagcctcgc tcgcgctcaa tccggcggag tggccgtagc gggtgttgtg
    10801 caggatccca gcagcgcgcc gattccggcc gccagcgtgg ttttggcgat gcccgggagc
    10861 agccaactgc aaaccacatc caccaacgct tctggggcgt tccggtttga ccgtgtgccg
    10921 ccgggcgagt atcaagtgcg ggtccaacac gacggcttta acctggcgac ggcctccctc
    10981 cgcgtcggcg cccggccgcc ttcgccgctg aagatcacgc tgacgctcgc cacggtgcgc
    11041 caggaggtga ccgtcagtga agagacgacc caggtgaata cggacacgtc gcagaaccag
    11101 gacgccgtca gcatggaccg gcaatccctc gatgatgtgc cgattttcga ccaggactac
    11161 gtgggcacga tgagccagtt tttggattcc aacctggtcg gaaccaatgg cgcgacgctg
    11221 gtcgtcgatg gacttgaagc caccagcgcc ggggtaacgg cctcggccat ccaggaagtc
    11281 cgcattaacc agaatccata ttcggcggaa tttccgcggc ctggccgtgg ccgcatcgaa
    11341 atcatcacca agcctggggc gccgcagtat cacggcgcct tcaatttcct ctttcgcgac
    11401 aactatctga atgcgcgaaa cgcttttgcc gccgtgcgtc cggccgagca gcggcgcatt
    11461 tttgagggct atttgaccgg ccccgccggc cacagccaga tgacttcatt cctgatctcc
    11521 gcgcaccggc aggaggaaga ttcgcagtcc attgtattcg cacaaggagt cggcggcgct
    11581 atccagcaga acgttcccag cccttcgcgc aacacgcaaa tcgcgggccg catcaaccac
    11641 aactacaccg gcggtagttc gatctcgctg ctctacgatt tcaaagaccg caccagcaac
    11701 aaccagaacg tcggcggcct gaccctgccc gaggccggcg tgaataccca gtttcgcgag
    11761 gacgaactgc gctggaccta caaagctgtg gtcacgcccg tgctgctgaa cgattttaca
    11821 gtcatgttcg ggcgcgctca cgctcccaca ttcagcatcc actccggacc gcaaattgtc
    11881 gtgcaaggcg cgttcaccgg gggcggagcg caggccgatc aactgcgaac cgagaatcac
    11941 gtcaacctga gtgaaatcgc ttcctggacg cacggcaggc actacgtacg ggccggcttt
    12001 cagattccag acttcagccg ccgcggtctg gatgacaata ccaaccgcgc cgggacgttt
    12061 tatttctcca cgctgcaaga ctacatgctc aaccggccgt actcttttgt gcagcagcag
    12121 ggcgacgggc gcgtcgtttt caccgagcac gtgttcggcg gtttcgtgca ggatgagatc
    12181 cggttgctgc cgaatctttc cgtgtcggct ggcctgcgct acgactggca gaattatttc
    12241 cgcgacaacg ataattttgc gccgcgaatt tcgttcgcct attctccgga caggcggcga
    12301 aagacggttt ttcgcggcgg cgccggcgta ttctacgacc gcaccggccc cgggccgatc
    12361 ttcgacctgc tgcgatacga cgggacgcgc ctgcgaagat acgtgatcac gaaccccggc
    12421 ttcccggatc ctctgagttc gggcgggccg ctcgcggcgc agccgaccag catcgtgcgg
    12481 ctggcgccgg acatacgcat tccttacacc ttgcaataca gcttcgcggt ggagcgccag
    12541 ttgcagcgcc ggacgacgct caccgtgacg tatttcggaa cccggggcgt cagcctgttc
    12601 cgttcgcgcg atttgaatgc gccgccgccg ccgttatatg cggcgcggcc ggaccctgat
    12661 ttcagcgtga tccggcaaat tgaatcttcc gggcgttcgg aaacgcattc actcgaggtt
    12721 tccctgcggg gcaatgtcag ccggtacttc aatggaatga tccaatatac ctacggccgc
    12781 gcttataaca acacgagcgg cattaactat ttcccggcga ataactatga tttgtccgga
    12841 gaatggtccc gggccgactg ggaccagcgg caccgcctga atctgtttgg aaccatcaaa
    12901 gctacaaaac ttttcaacct tggcgttgcg ctttggatgc actccggcgc tccctatacg
    12961 atgaccaccg gttttgacga caatcatgat ggcgtcgcca ccgaccggcc gcccggcgtt
    13021 ccgcgcaaca gcttgcaggg accgttttac gcggatttcg atttgcgctg gtggcacgac
    13081 tttctactgg agaaatccaa gaaagagaaa ggtcctgtgg ctaccatcgg gctggacgcc
    13141 ttcaatgtca taaaccgggt caattacacc aacttcgtag gcgacatcag ctctccgttt
    13201 ttcggccgcg ctgtttccgc gcagccgcct cgccgcttgc aggcgtcctt ccggttgcgc
    13261 ttttgagcga agccgttgcg gtaagccggc ctagtattcg actttcacga gaaataatcc
    13321 gcgagcggcg gcggtcgggc cggcgcggct gcggtctgcg gcggccagaa tggcgggcac
    13381 gtcgccgggg gtcagattcc cctttccgat ttccagcaag gttccaacta tgttgcgcac
    13441 catgtgatgc aagaatcccg agccgcacac ggtgtagcgc agcaggtctc cttccggctc
    13501 caggcgcgaa gaaaagatgg tgcggacggc gccgggctcg gcgggatcag cggcggcgaa
    13561 gctgcgaaaa tcgtgggttc cctcaaacag cggagcggct tgcaccatat ccgccactcg
    13621 caacgggtac gggtgatgat aaacgtagcg gtacatcaat gggtggcaca cttcaccgcg
    13681 ccacaaacgg tattcgtaaa ctttcgatcg cgccgagaag cgggcatgaa acgccgggcc
    13741 ggcttcttca acctgcaaaa tgcgaatgga gggcggcaac agccggttca ttgcccggcg
    13801 gaggttggtt gtgggaatag ggttttgaag tttgacgctg gcgacctgct cgagcgcgtg
    13861 gactccggcg tcggttctgc cggatcccgc tacggcgacc ggctttccct caatttcgcg
    13921 caagacgcgt tccagttcgc cttggatggt cggtagatcg ggctgaatct gccagccgtg
    13981 gaaatccgtg ccatcgtagg ccagcgtgag cttgagattc cgcacaagaa tcagcgaaga
    14041 tcagaccggc gggcagggcc gttcaggaaa agcgcatgtg atcccgcaga gggccgcggg
    14101 aattggcggg atcgtcatcc tttccggctt cctggcttga tcacggggag cccttgcgcg
    14161 cataatgggc acgcatccgg agcataggtc tgaacggtca gagtggcgag ggccacgcga
    14221 ggcacgccga cgtccgcgcg gccgccgctg cggtcgataa tcgatccggc ggccacgacg
    14281 gtccctccag cctgctgcac ggcggcaatc gtttccgccg tgctgccgcc cgtggtgatc
    14341 acgtcttcaa tcgctacgcc gcgagcgccg ggctcgaccg caaaaccccg gcgaagcgcc
    14401 atgcggccgt cgctgtcacg ctccgtgaat aagaaccggc agccgagcgc ccgcgctact
    14461 tcgtggccga tgatcaagcc tcccaaagcg ggcgcgatga cgaaatcggc gggccttccg
    14521 gccaggagcg gacccagggc ctgcgctaaa gccgctccca gccgcccggc cgtcgaggga
    14581 caggcgagca gcaacgcgca ttgcagatat tcaggactgt gcagtccgct ggtgaggcgg
    14641 aagtggccct ggagataagc gcctgtgtgc cggcacagct cgagcacctc ggcctgtgcg
    14701 attcgctcag cagccataaa attgagatcc tgccgccgga atgctaggat agcaaatgac
    14761 cttagcccgt gtgggccaca ggaaacatgg gggaaccggg tgggaacttc ccggaagttg
    14821 tcttcgtagg taacagagac agccgtggga gcgccatatc gttttcgcgc tcgagcaggc
    14881 aatctgccgg gcgccgaccc gaatgaattc tggagtgcat taacatgcgt ttgaaagctg
    14941 ttttcgctag ccttctcagt gtccttatgg tgcagccggt gtggggacag ggggcgcctt
    15001 cgcccgctcc cgccgagcct gcggtcgcgc aatctcagaa gataagccag cccgattata
    15061 cgaagggcaa catatggtgg cccaatttta ccagcgtgtt ccgcacgccc tatgtggccg
    15121 aggttaacat ggcgaacagc aaccgcctgg agcaattgct gcgcgacggc aacctttacc
    15181 tttcgctgga ggatgcgatc gctctggcgc tcgagaataa cctcgacatc gcctacacgc
    15241 gctatcaggg gccgatcgcg gacaccgata ttctgcgcag caaggcggga gttccgatcc
    15301 gcgggctggt gaatccgggc cagatttcca gtttctcgac gggcaattcg gccaccggcg
    15361 ccaatggcgt gaatccgatc ggcagcacga ccaacgcaag cacgatcagc acgttcgcga
    15421 caggctcgac gatcaccggc ccgaacggcc tgtccgtagt tggaacgaat atcttgggag
    15481 gcgctccgct gccccagttg gaccctgttc tgaccacaac cgtcggctgg gcgcacaaca
    15541 gcaccccgca atccaatacg gttatcacag gcaccaatac gctcatctcc cagggcgcga
    15601 gtactcagat cgcgtactcc caggcttttt tgacgggcac ctcgttcagc ctgtcgttta
    15661 acagcgcgac cagtaactcc aacgcgatcc ggaacacgct gaatcccgcg ctcaacagca
    15721 gcgccaccct gaatttcacc cagcctctgc tgcaaggttt tgggcgctcg atgaatgcgc
    15781 gctatctgcg gatcgcccgc aataaccgtg aagtggccga cctgcttttc aaacagcagg
    15841 tgattcagac ggtatcgcag gtgcagaacc tctactggga tttggtggga ttcaacgaga
    15901 acgtgcgcgt ggtgcagcag gcgctccaat tggcggaaaa gctctatgcg gacaacaaga
    15961 gacaggtgga aatcgggact ctggcgccga ttgagatcgt gagagcggaa gcagaagtgg
    16021 ccgcgcggca gcaggacttg acgctggccc aaacccaggt gcagcagcag gaggtgattc
    16081 tgctgaacgc cctgagcaaa aatggcgtgg ccagcccctc ggtggcccag gcccatatta
    16141 ttcctaccac gcgcctgacg gttccacccg tcgagccgat cgagccggtg caggatttga
    16201 tcagcatggc cctgcaatcg cggccggaac tcgcccaatc gcgaatcggg ctgaccaatt
    16261 cggatatcgc cctgaagggc actcgaagcg cgatgctgcc gcaattgaac ctggttggca
    16321 cgcttaccaa caacgcctta gccggagaag tcaatgaaca cttcgtcgcg atcggcggcg
    16381 gcagcgcgcc tccgtcgccg ttcttcgtcg gcggcttcgg aacggctctg agccagattt
    16441 tttcccgtaa ctttccgact tacagttttg gcgtgcagtt gaacgtcccg atccggaacc
    16501 gggcggctca ggccgacatg gcagccgcgc agctccagca ccgccaggcg gagattcgat
    16561 tgcgccaggc cgaaaacgag gtgcggctcg aggtaaaaaa tgcgctggtc ggtttgcagc
    16621 aggcccgcgc ccgcatggat gcagccacaa aatcccgcat attggaagag cagactctcg
    16681 acgcggagca gaagaagtac gctctgggcg cctcgaccat cttcaacgtg attcaggttc
    16741 agcgggacct ggcttcggcc cgggaaatcg aggttgccgc catcaataac tatgccaaag
    16801 cgcgcgtgga gctagatcgc gcgaccggcc agacgctcgc gaagaacaac atttcgatgg
    16861 aagaagcctt cgagggcaac gtgagccggc cgccggatcc gatccctgcc aacccgccgg
    16921 cgcccgttgc ggcgcccgct ccgccgtccc cggcgcccgc tccgtgatct gaaagcactt
    16981 ttaccggctc cggcggctgc tccccaagca gccggagccg cgtgacagca cgggaccggc
    17041 atagggccac tccactccaa acgtttccgg cgccctcgaa ccggaactgc acgcttactt
    17101 cagggcgccg atgctttttt gaatgtcttc gtaggccgtc ttgtcaatct cgtatgtggt
    17161 gggctcgttt tcgcgggcgg cgtaatattt ggattcgggg ccggccgcca tgagcacatg
    17221 ctcgacgcgc tttccgtcgt ccgaagtcac tttcgcctcg agcgtgggct tgttcaagcc
    17281 atacctcgac tgggctgaag cctcgtcgga cggaaaagaa gtcgcgctga gattgcgcaa
    17341 atggtcgatc agcgtctgca ccttgtccgc gggcagctct ttgctgccag cgtcagtgcg
    17401 cagccatttg ccgtttttct tttcaatgac catctgcgag gagccatcgc gcacttcaac
    17461 tttggccggg tcgttgaatc cgaagtcaaa gagctttcgg ttgcgaagac tgtccagatt
    17521 cttgttcaag gcgtcgggca cggtactcga tatctggaaa acaccgggca tcgccgtggt
    17581 cttggcgtaa tacgtatagg tagtgctgtc tttctgtctt tcccgcccca cggtgagcgc
    17641 ataggttccc gacgcgtcgg ttacttcgag gacttcgtaa ggcgaagaaa acgaatattt
    17701 ggcgggcggt tttccgcttt cgtcgatgac cgaatcgaaa atgagattgt gagcggaccg
    17761 caccatatca tcgacggcga acgtatcggc ccgcatgagg cgcggcttca agatctgcca
    17821 gtccgacccc gccttgctga actcgagcgt ctggccgttg gcggtgagaa cgactcgcgc
    17881 taccttagag tcgtcgaacg gcatcaggtg gcggtcgcgc acatccacca gccgctcatc
    17941 cagactggcc ttcacatagc tgggaaccgt gaaaagcttg ccatcctgat tcagcctcgc
    18001 ataaaccacg gaggaggccg gggcgtccag cccgaagctg actttgttct gcttgccgtc
    18061 tttgagcgtc atggtgacga ccagcttcgg cgggtcaaga ccgtaggtcg cccaatccgt
    18121 gttgttttcg gaaagaacct ggtcggccgt gagagacgtc aagttgctca gcatggtgtt
    18181 gagagcggtc tcatcggcct tgtacacttt cggttcagtg accttccatt tcccgtcatc
    18241 gcccttttca agcgtaaatt cgtcgccgct gccgcccggc ctggtaaact gcaagcgttt
    18301 cacctgatcc tgcggaaccg tcagaatctt ctggctggcg gtgttggttt tggtttcggg
    18361 tgaaggcggg tgtctctcag agtaccagac cgccccgccc aggccgccca aaatgacgac
    18421 agcaatcaac aagcgcagcg gtttcacggc tcgttacctt cggcgccacc agacgtaggt
    18481 tcccccgacg atgatcagca gcgggaatcc tatcaccgag gcgtagaaaa tacgcgccat
    18541 ctgcgagacg gtgagcgaga gccggcgatc ttccgattcc ttggggcgga tggaaatcag
    18601 gtcctcgtcg gagctcagcc agttcatcat gttgaggaag agatcgcgat tgccgcccgg
    18661 gaaccgcaag gtggcgttca cagcaaaacg cgagcttccc accactacga agcgcccttg
    18721 cacgttttct ttatccgtgc gatacgttcc ggcgaccgcc agagagaagg gccccgtctt
    18781 atctctcttg ggatcgagct tgatctggcc cgaggcgaag ttgctggtcg cgaagctccg
    18841 ggaggtagtc tggaacaact tttcccgcaa gacattgctg ttcctcgaat caggatcagg
    18901 ctccacgctc cgggaaaggg ggaacaggca cgccacgttc ttcatgtcct gggtgatcgg
    18961 ctgggattca tagctggtga cgagaggcga aagctcgtca gcgccgaaaa gctggccgac
    19021 gccgcttagg tccaccacaa tgttattgtc cactttgacg ccccagtcgc cgatcatttt
    19081 gtcgagggcg ctggtgttga cgcctttcgt ctccggatca atgaggaaca gggcgcgccc
    19141 gccgccctcc acgtacttct tgatagcggc gatctccaca tcgagcagat tattcttggg
    19201 cccagcttcc acgagcacgg tgcagtcgct gggaacggct gcgttctttt cgagcaggga
    19261 gatggtcttg gtggtgaaat tcgtagcttc cagagcatct ttagcgccgg aaaagccgtt
    19321 gggctcggag ctggagatgt ccgcttctcc atgtcccgtc acgaagcacg ccgtacgctt
    19381 gccgcccttc agcgcccgga tcagagcgtt ggtgacttgc tcttcggttg ggctcttcgc
    19441 ctcctcgcgc cgcccgttta cctcgactat cgtggtgttg gtctgggtga ctttcatgtc
    19501 cagcgcccgc tttgggttct tcaccggatc gacgaagtcc accgtcacgt aatgggacgc
    19561 gctgctgtag cgttgcagca aatcccgagg gctggggccg aaagcgctct cgttcgtctg
    19621 gttggaagta ttgaaatagt aaatagtgac gttctgtttc aaattggcca gtatcttctt
    19681 ggtctgatcc gagaggctga acaccttgtt gctcgtcgcg tcgtaggttt tatcgtatcg
    19741 gttggccagg aaattggcgg cgaacaagat cgccagcaca accacgagat agacggccac
    19801 ataagcgcca tatttggtct ggcgcgcgct gatccacccc attttcatgc gatcatgccc
    19861 tccaacgcaa agattccatg gatcgggcag tcagaaagat gcccagaaag ctcaccgaga
    19921 ggtagtaaac cacgtccttc aggtcgagca cacccttggc gaactgctcg aagtgcgtgg
    19981 tgatggaaag ataggaagcc agcttgccgc ccgttccggt ggagaacgag cttacccagt
    20041 cgagcaccca cagcagtaga aacacgccaa acgtcaggct tgccgccacg atctgattct
    20101 tcgtgagcgt cgatagaaac gaaccgaggc cgagaaacgc ggcgcccagc agaatcaatc
    20161 ccagatagcc caccaggagc gcccgccagt cgggctgacc gtaccggaac aaaaacagga
    20221 tgttcagggc ggagatcccg atgagacaga gatagaggag catggcggcg agccatttgc
    20281 ccaggatgat ttccgtgtcg cgcaggggag aggtgagcag cagctcgatg gtgccgctgc
    20341 gtttttcctc cgcaaacagc cgcatggtga tcatcggcat cagaaacaga acgatcacgc
    20401 tcacattcat cagcagcggc cggatgatga agtcgttcat attcatcgga gggccgcccg
    20461 gctgcatcat cgaccgcgca ctgtagtcca cgaacagcgc cgtggcgctg taaaagaaat
    20521 aaccgaagat gacggcgaac atcgccagca caatataggc gatgggcgag gcaaaatagc
    20581 tgttcaattc ccgtttcgca agcacccaga cgtttttcat gctgcgcctc cggaggctct
    20641 tcgctccgct gcggcgggct cagcggccac gtcggcggtg gtcagtttca ggaatatttc
    20701 ttccagactc agccccacag gccgcaactc gtgcagcccc cagcccgcgg tgaccactgc
    20761 gcgcgccagc tcgctgcgta tgttgtggtc cttggcggtt tcgatctccc aactgcaacg
    20821 cccggcctgc ccgtccttgc gcacgacgcg gttcacgccc tgaagagagc tcaggcggga
    20881 cgcgacctga tcggccggcc cctctacatc cagaaagatc gtctcatgcc cttgcaaacg
    20941 cgcggtcagg ttctcgggcg tgtcgatcgc ttccagcttg ccttgattga tgatgatcac
    21001 caactggcag gtgctggaga cctcgggaag gatgtgcgtg ctcaaaatga ttgtgtgctc
    21061 gccggagaga tcgtgaatca gcttgcgcgt ttcgataatt tgcttggggt ccaggccggc
    21121 cgtgggctcg tcgagaatca gcacatcggg gttatggatc agcgcctgcg ccaggcccac
    21181 ccgctgccgg tagcccttcg acaacttgct gatcagcttg gagcgcactt cgcccagcgc
    21241 gcagcgctcg acaacctcat tcacccgccg cttaatgttg gatgccggga ctcccttgat
    21301 tcgcccggcg aatacgagat attcatccac ttccatctcg gggtatagtg gaggcgtctc
    21361 gggcaaatag ccgatatgac gcttgacttc gagcggctgc tcgagaacgt cgtatcctgc
    21421 aacactggct tttccttcgg tgggaggcaa gaagcaggtc agaacgcgca tagtggtggt
    21481 ctttcccgcg ccgttcggcc ccaggaaacc cacgatctga cccttctcga cttcaaaact
    21541 tatgtgatct acagccgtag cccggccata gcgcttggtc agaccttgca cttt
//
LOCUS       DASREK010000269         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1456475, whole genome shotgun sequence.
ACCESSION   DASREK010000269 DASREK010000000
VERSION     DASREK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456475"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..429)
                     /locus_tag="VFA54_14630"
     CDS             complement(172..429)
                     /locus_tag="VFA54_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZS52099.1"
                     /translation="MDQDLAQRVIAIIAKTQHLDTSKVTLDSSYEELGIDSLDGVNIL
                     FALENELDIQIPDEAAKQIRSVRQTVEGVEKLLAQKTAGAG"
     gene            617..1930
                     /gene="thrC"
                     /locus_tag="VFA54_14635"
     CDS             617..1930
                     /gene="thrC"
                     /locus_tag="VFA54_14635"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZS52100.1"
                     /translation="MPGRLLCFNPSCRTPFSITDVLYTCPKCGGLLEAAYEFRGAAPE
                     TVRCLWRERRLSDAPLDRSGVWRYREMLPFLDDYSRVVTLREGNTPLLDAPAAARYAG
                     LDGLAFKHQGYNPSGSFKDNGMTAGVAQALRLGMEIVACVSTGNTSASMAAYAAAGGL
                     KAVIFIPRGNIAYGKLAQALEYGALTFQVEANFDQILRLVRDLAEKEGIYLLNSVNPF
                     RIEGQKSIVIEMLEQRNWKPPDWIVLPGGNLGNTSAFGKALRELQAVGLIARLPRLAV
                     VQAEGASPFYDLFQRGGQGEIRPVEHPETLATAIKIGAPVSWPKALYEVQTTGGVVEK
                     VTEQEIADAKAQIGRCGIGCEPASAATLAGIKKMAATGAMRRDADVVAVLTGHLLKDP
                     DYIYRYHTGQLEGPDGRRLKSTFGNQPIVVPNDPVKIAALLKDKT"
     gene            1948..2415
                     /locus_tag="VFA54_14640"
     CDS             1948..2415
                     /locus_tag="VFA54_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZS52101.1"
                     /translation="MAHINYAIDFTVACFIVKDDCVLLVFHRNLQKWLPIGGHIDPGE
                     DPDQALFREIQEEAGVEVEIAGQKAPINDSSARALYTPAHVNIHRITETHSHVVNIYY
                     ARWVGREPVLCEKEHQEIKWFSRIDLRNPRFGIKPDVLWYAEDALDKLSARQQ"
     gene            complement(3072..>3563)
                     /locus_tag="VFA54_14645"
     CDS             complement(3072..>3563)
                     /locus_tag="VFA54_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52102.1"
                     /translation="YSAIEEAKADVTGLFLLQYLFDKGYLPGDRAQNERRLYTTFLAS
                     SFRTLRFGLQDAHAKGIAMQFNYLTDKGAFVAGPAGTFAVNFSRIKPAVRDLAHDLLT
                     IEATGDYEGAKKMLDQLGVVRPGAAAAMNRLKSLPTDIEPLFATATALLRTTPAKAVR
                     KKR"
BASE COUNT          724 a         1032 c         1088 g          719 t
ORIGIN      
        1 catacccacg cacctgagcg gccactttga tcgacagacc attggcagga aacccctcca
       61 gcggaccaat gcccgagcgg ccggcgcaaa ggttatccca aaaggcgccg gtatttggac
      121 ccagcgccga tatggttcct ataccggtca ccacaacccg gcgcatagga gtcatccggc
      181 gccggctgtt ttctgagcca gcagtttttc gacgccctca acggtctggc gcacgctgcg
      241 aatctgtttg gcggcttcgt ccgggatttg aatgtccaat tcgttttcca acgcaaagag
      301 aatattgacg ccgtccaacg aatctatgcc cagctcttcg taggatgagt ccagcgtcac
      361 cttagacgtg tccaagtgct gcgttttcgc aatgatcgcg attacccgct gtgctaaatc
      421 ctggtccata cgctggggtc atcatacaac aagccggcct gtatttgtgc ttgtgtgggc
      481 gcggccggct gttctttttg ggccggtgaa cgtgccggag aacaaaaggg gcaacaggcc
      541 ccgcccggcg cgccggtcat tcagggaatg cgctgcctta cactccggcc agcgcttttt
      601 aatatgatgg acctttatgc cgggtcgcct gctctgcttc aacccgtcct gccggactcc
      661 gttttccatc acggatgtgc tttacacttg tccgaagtgc ggcggcttgc tcgaagccgc
      721 ctatgagttt cgtggtgcag ctcccgaaac ggtgcgctgc ctgtggcgcg aacggcggct
      781 gagcgatgcg ccgcttgatc gaagcggcgt ctggcggtat cgcgaaatgt tgccgttcct
      841 ggacgattat agccgcgttg tgacgctccg cgaaggcaat accccgcttc tcgacgcgcc
      901 tgcggcagcc cgttacgccg gcctcgacgg cctcgctttc aagcatcagg gctacaaccc
      961 ctcgggatcg tttaaagaca acggcatgac tgcgggcgtg gcgcaggctc tgcgcctcgg
     1021 catggagatt gtggcctgtg tttccacggg gaatacgtcg gcttcgatgg ctgcttacgc
     1081 agccgccgga gggctgaagg cggtgatttt tattccccgc gggaacatcg cctacggcaa
     1141 actggcgcag gccctcgagt acggagcttt gacgtttcag gtagaggcca acttcgatca
     1201 aatcttgcgt ttagtgcgcg atctggctga gaaagaaggt atttatcttc tgaactcagt
     1261 gaatccgttc cgtattgagg gccaaaaaag cattgtcatc gagatgctcg agcagcggaa
     1321 ctggaaaccg ccggactgga tcgttctgcc gggaggaaat ctgggaaata catcggcatt
     1381 cggtaaggcc ttgcgagagc ttcaggcagt cgggctgatt gcccggttgc cccggctagc
     1441 cgtggtgcaa gccgaaggag cgagtccgtt ttatgacctg ttccagcgcg gcggccaagg
     1501 ggaaattcgc cctgtcgagc atcctgaaac tctagccacg gcgatcaaga tcggcgcacc
     1561 tgtttcctgg cccaaggccc tttatgaagt gcaaacgaca ggcggcgtcg tggaaaaagt
     1621 aacggaacag gaaattgccg atgcgaaagc gcagatcggc cgctgcggca ttggctgcga
     1681 gccggcttcc gcagccaccc tggccggcat aaaaaaaatg gctgccacgg gcgcaatgcg
     1741 ccgggatgcc gacgtggtgg cagtattgac cggacatttg ctcaaggacc ctgattacat
     1801 ctaccgctat cacaccgggc agctcgaagg cccggacggc cgccgcctga agtcaacctt
     1861 tggcaatcag ccgatcgtcg tcccgaacga tcctgtgaag atcgcggctc tgctaaaaga
     1921 caagacctga agcgccgggg gacgcggttg gcgcacatca actacgcaat cgatttcacc
     1981 gtcgcctgct tcatcgtgaa ggacgattgt gtactcctcg ttttccaccg aaaccttcag
     2041 aaatggcttc ccattggggg ccacatcgat ccgggcgagg accccgacca ggcgctcttc
     2101 cgcgagattc aagaggaagc cggagtggaa gtggagatcg ccggccaaaa agctccgatc
     2161 aacgattcct ccgcccgggc cctctacacg ccggcgcacg tgaatatcca tcgcatcacc
     2221 gagacgcaca gccacgtggt caacatttat tacgcgcggt gggtcggcag agagccggtg
     2281 ctatgcgaaa aagagcatca ggagatcaag tggttcagca ggatcgacct gagaaatccg
     2341 cggtttggaa tcaaaccgga tgtgctttgg tatgctgaag acgctctgga taaactcagt
     2401 gcgcgccagc agtgaggcag cggctcatag gatccgttag cggcgcggcg gcagaactga
     2461 tccaaaatat agccggtttg gccgccgaga tccgtgactt gcagcagcac tccgcttccg
     2521 gaagcccgcg cagagaccga gaaggccagt tcaaccaaac cgtattggtt gcgtaatcgc
     2581 ggaagctcag aggcgggatc agccgcgcga accaggatcg gccgcccgtc ttcttgaata
     2641 gcggcgagat atgaggcggg ttcatggccg gagaggatca aatttctcaa ggtctccaaa
     2701 aggcccaaca tcacggagaa ggccgataag gacgcgctgg gccggagggc ttgcatattc
     2761 tcgactggcc tcccgcgttg ggccgttcca agacccgccg gagtacggtt gggctttccg
     2821 aagatcggat gcgggatcag gcttccttgc gcgcccatgt gtcccttatc gtccgcaaaa
     2881 cgaaaactta aagcagaatt ccgcggggtg gtaaaaatgt gagagcggcc tttctgcggc
     2941 tggcccgggt gcgccggctt cgaaatttca gtgtctcccg gcgccgggtg tttcgggttg
     3001 agcgccagcg gcgccgctct tggcgcgagc acgggcggtt gagccggaac ggaagcccgg
     3061 tttgccgatg ctcaacgctt ctttcggacg gctttggccg gcgtggtgcg caggagggct
     3121 gtggcggtcg caaaaagcgg ctcaatatcg gtggggaggc tcttgagccg gttcattgcc
     3181 gccgcagcgc cgggccgcac cacaccgagt tggtcgagca ttttcttcgc cccttcgtag
     3241 tcgccggtgg cttcgatggt caacaggtcg tgggccaaat cccgcacggc cggcttgatc
     3301 cttgaaaaat tcacggcgaa ggttcccgcc ggaccagcga cgaacgcgcc cttgtcggtc
     3361 agataattga actgcatcgc tatgcctttg gcgtgggcgt cctgcaatcc gaagcgcagc
     3421 gtgcggaagg acgaggccag gaaagtcgtg tagagccgcc gctcgttctg cgcgcgatcg
     3481 cccggcaaat agcctttgtc gaataaatat tgcagcagga acaggccggt gacgtccgcc
     3541 ttggcctctt cgatcgcgct gta
//
LOCUS       DASREK010000270         4233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2110988, whole genome shotgun sequence.
ACCESSION   DASREK010000270 DASREK010000000
VERSION     DASREK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2110988"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFA54_14650"
     CDS             <1..309
                     /locus_tag="VFA54_14650"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS52103.1"
                     /translation="IAALPGVDALMIGPFDLSISLGIPGQFDHPVFWKAFDRMVEACN
                     KAGIAPGIHMANAKSLKLAAERGARFLVCGSDASVMLAGYRSIVEQLELAAVSKAGYM
                     "
     gene            complement(371..1468)
                     /locus_tag="VFA54_14655"
     CDS             complement(371..1468)
                     /locus_tag="VFA54_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52104.1"
                     /translation="MRHWIEAHRRFLLLPAALLLACSAARSAEIRTIAGTGKPGYSGD
                     GGPGVSGEVNNPYGLTIGPDGALYFCEVDNNVVRRLDLKTNRLSTVAGNGRKGYSGDG
                     GPALAASLNQPYEVRFDRAGNMLFAEMQNHVIRRVDAKTHTISTLAGTGAPGFSGDGG
                     PASQAQLRQPHSIAIDSAGLLYIADIGNHRIRRVNLQTGVIETFAGTGERKPTPDGAS
                     LDGVPLNGPRAMAFDRNGDMFLVLREGNAVYRIDMRRRVIHHLAGTGAQGYSGDGGPA
                     RDALLSGPKAISIAPGGAIFIADTESHTIRRIDLQKGTIETVVGTGQRGDGPDGPPRS
                     CRLARPHGVFVDPSGVIYIADSENHRIRVLK"
     gene            complement(1575..1799)
                     /locus_tag="VFA54_14660"
     CDS             complement(1575..1799)
                     /locus_tag="VFA54_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52105.1"
                     /translation="MRVEEHQTRTLEIDGWPVRLTSYRLGDEFHCTADNVDPGAWLAR
                     TKGSTREEAENLAIERARKLLARTRKTGIS"
     gene            complement(1818..2675)
                     /locus_tag="VFA54_14665"
     CDS             complement(1818..2675)
                     /locus_tag="VFA54_14665"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZS52106.1"
                     /translation="MTDYKHPEVLVSTDWVEQNLDNPKVRIAEVDVDTKAYEEGHVPG
                     AIGWAWNTQLCDTVRRDIIPKADFEKLMGSSGIANDTTVILYGDNNNWFAAWAFWQMK
                     IYGHRDVRLMNGGRKKWLAEGRELTKDAPKIAPVTYKAQDPDFSIRAYLPQVQQSIAG
                     DGVDLVDVRSPQEFTGEILAPPGLPETCQRGGHIPGARNIPWSQACNEDGTFKSADEL
                     KALYERAGITGQRPTIAYCRIGERSSHTWFVLTYLLGYPNVTNYDGSWTEWGNLVGAP
                     VEKGASPAR"
     gene            complement(2761..>4233)
                     /locus_tag="VFA54_14670"
     CDS             complement(2761..>4233)
                     /locus_tag="VFA54_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZS52107.1"
                     /translation="TSGTTRPQTPATPERRPVTPGDEEELRRAVQQFTSAYLAVEENF
                     ADPVVPEQAFYGGAIPGLLRSLDPFSSFFDPEQFQSLREMQQSVQKGFGSVVSIAPGR
                     VIVLQTLPGTPSARAGLSAGDEIVEVNGYRLDRLELEQLVELLQQSRQRPAQLVVRRP
                     GIATLMTFQLTPAELASPSVSRSFFLQPGVGYIKIESFEEATGGELRKALDKLGGEKL
                     RGLVLDLRDNPGGIVPAALETASLFLQPGQLILTVRGRAGEPEEIKVPDGVKPFQFPL
                     AVIVNGKTASASEIVAGALQDHDRATIVGEPTFGKGLVQRVLELSEGTGLALTTALYF
                     TPSGRSIQRPLEQLKNPNGGLGDSAAVFHTTEGREVKGGGGITPDVLVAPEGLNPFRA
                     TLEATSSFLNFAQQYTAQHRNVSAAFEVTPDLLDEFQSFLSERRIRPPLSEWSANREY
                     IELRLKEEIFNLALGVEKGDEIAARRDVQIQRALQALKLP"
BASE COUNT          776 a         1316 c         1300 g          841 t
ORIGIN      
        1 atcgccgctc tgcccggggt ggatgcgctc atgatcggcc cgttcgatct ttctatttcc
       61 ctgggaattc ccggccaatt cgaccatcct gtcttttgga aggcgtttga ccgcatggtc
      121 gaagcgtgca ataaggcggg aatcgcccct ggcattcaca tggcgaatgc caaatcgctc
      181 aagctggccg ccgaacgggg cgcgcggttc ctggtttgcg gctcggacgc ttccgtgatg
      241 ctcgccggct atcgctcgat tgtcgagcaa ctggaactgg cggcggtgag caaagccggc
      301 tacatgtaga ggctggaaaa gtctccgctg ggcggaagtg accccggccc aagggaatgg
      361 cagttcccgg ctacttgagg actcggattc ggtggttctc gctgtccgcg atgtagatca
      421 cgccgctcgg gtccacaaag acgccatggg gccgggcaag acggcaggag cgcggcggcc
      481 catccggacc atcgccgcgc tggccggttc ccacgacagt ttctatcgtt cccttctgca
      541 agtcgatccg ccggatcgta tggctctcgg tgtcggcgat gaatatggcg ccgccgggag
      601 cgatcgagat ggccttcgga ccggaaagca gcgcgtctcg ggccggcccc ccgtcgccgc
      661 tatagccttg cgcgcccgtg ccggccagat ggtgaatcac ccgccgacgc atgtcgatgc
      721 gatagacggc attgccttcc cgtaagacca ggaacatatc tccattccgg tcaaaggcca
      781 tggcccgagg gccgttcaac ggaactccgt cgagggacgc gccgtcggga gtcggcttgc
      841 gctccccggt tccggcgaag gtttcgatca cgcctgtttg caggttcacc cgccggatac
      901 ggtgatttcc aatatccgcg atatagagca aacctgcgga atcgatggca atgctgtgcg
      961 gctggcgcaa ttgcgcctgg ctggccggtc cgccgtcacc ggaaaaaccg ggtgcgcccg
     1021 tgccggcgag cgtgctgatg gtatgcgtct tcgcgtccac ccgccggata acgtgatttt
     1081 gcatttcggc gaaaagcatg ttacctgcac ggtcgaaacg gacctcatac ggctgattca
     1141 gagaggctgc cagtgccggc ccgccgtcgc ccgaatatcc cttacggccg ttgccagcca
     1201 ctgtcgaaag ccggttcgtt ttcagatcga gtcggcggac gacattgttg tccacctcgc
     1261 agaaataaag agctccgtcc gggccaatgg tcaacccata cgggttgttg acctccccgg
     1321 agacgcctgg accgccatct ccggagtagc cgggtttgcc agtgccggcg atggtccgga
     1381 tttcggcgga acgcgccgca gagcaagcca gcagcagagc cgctggaagc agaagaaagc
     1441 gccggtgggc ttcaatccaa tgcctcatag gtcacaacct ccgaggatag gccgcgggtt
     1501 cctgcacagc cacatcgtcc aggcgcccaa attcgagcgc gtccccgcga ggaacatagt
     1561 atctgggttg tcggtcagga gatccccgtc tttcgcgttc gcgccagcag cttgcgcgcg
     1621 cgttcgatag caaggttctc agcctcttct ctggtagacc ccttggtccg cgcgagccac
     1681 gctccggggt ccacattgtc cgcggtgcag tggaattcat ccccgagccg gtagctggtc
     1741 agccggaccg gccacccgtc gatttctaat gttctggtct gatgttcttc cactctcatg
     1801 gctgcggggc gcacagttca gcgcgcaggg ctcgccccct tttctacggg cgcgccaacc
     1861 aggttgcccc attcggtcca tgagccgtcg tagttggtca cattgggata accgagcaga
     1921 tacgtcagga cgaaccaggt gtggctagag cgctcgccga tacggcagta ggcaatcgtg
     1981 ggccgctggc cggtgatgcc ggctcgctcg taaagagctt tcagctcgtc agcgctcttg
     2041 aacgtgccat cctcgttgca ggcttgtgac caggggatat tccgcgctcc ggggatatga
     2101 ccgccgcgct ggcaggtttc cggcaaaccg ggcggcgcca gaatttcccc ggtaaattcc
     2161 tgagggctgc gaacgtccac caggtccacg ccgtcgccgg cgatgctttg ctgcacttgc
     2221 ggcaggtagg cgcggatgga aaaatccgga tcctgggctt tataggtaac cggggcgatc
     2281 ttgggagcat ctttggtgag ctcccggccc tccgccagcc acttctttcg cccgccgttc
     2341 atcagccgca cgtcacggtg gccgtagatt ttcatttgcc agaacgccca ggccgcaaac
     2401 cagttgttgt tatctccgta caggatgacg gtcgtgtcgt tagcgattcc ggagctgccc
     2461 atgagctttt caaagtcagc tttgggaatg atgtcacgcc gcacggtgtc gcagagctgc
     2521 gtgttccagg cccagccgat tgcgcctggg acatgcccct cctcgtaggc tttcgtgtcg
     2581 acatccactt ccgcgatgcg caccttggga ttatcgagat tctgctccac ccagtcagtc
     2641 gaaaccagga cttccggatg tttgtagtcg gtcatagctt ttcctccggc gtctcgatgt
     2701 tgattcgctt gtcttattta ggagaaagaa taccacacac cctaagagat agccggccgc
     2761 ttagggcaat ttcagagcct gcaaagccct ttggatctgc acgtcgcgcc gggcggcaat
     2821 ttcgtcgccc ttttccactc ccagcgcgag attgaaaatc tcctctttga gccgcagctc
     2881 gatgtactcg cggttcgcgc tccactcgct taacggaggc cgaatccggc gctcggaaag
     2941 gaagctctgg aattcatcga gcaggtcggg agtaacctcg aaagcagcgg agacatttcg
     3001 atgctgcgcc gtatattgct gggcaaaatt caaaaacgac gaggtcgcct ccagggtggc
     3061 gcgaaacgga ttcaagcctt ccggcgctac cagcacatcc ggcgtaatgc ctccgcctcc
     3121 cttaacttcc ctgccctcgg tggtatggaa gacagcggcg gagtccccga gtccgccatt
     3181 cgggtttttg agctgctcga gcgggcgctg gatcgagcgc ccgctcgggg tgaagtagag
     3241 agctgtagtc aaggcgaggc ccgtaccctc ggagagctcc aggacgcgct gcaccaagcc
     3301 tttgccgaac gtcggctcgc ccacgatcgt ggcccggtcg tggtcctgta aagcgccggc
     3361 cacaatctcg gaagccgagg ccgttttgcc gttaacgatc acagccagcg ggaactggaa
     3421 cggcttgacg ccgtcaggaa ccttgatttc ctccggctcc ccggctcgcc cgcggacggt
     3481 gagaatcagt tgacccggct gcaaaaacaa ggaagctgtt tccagggcgg ccggcacaat
     3541 gccacccggg ttgtcgcgga ggtccaacac caagccgcgc agtttttccc ctccgagctt
     3601 gtcgagcgct tttcgcagtt cgccgccggt ggcctcctca aagctctcga ttttgatata
     3661 cccgacgccc ggttgcagga agaaggagcg tgagacgctc ggagacgcca gttccgcggg
     3721 ggtcaattgg aaggtcatca gcgtcgcgat gccggggcgc cgaaccacaa gctgcgcggg
     3781 ccgctggcgg gactgctgca acagctcgac gagctgctcg agctccagcc ggtccagccg
     3841 gtatccgttg acttccacga tttcatcccc ggcagagagg ccggctctgg cggagggcgt
     3901 gccgggcagt gtctgcaaca cgatcacgcg tcccggagcg atggaaacaa cactgccgaa
     3961 acccttctgc acagactgct gcatctcacg caaggactgg aactgctcgg ggtcaaaaaa
     4021 cgacgaaaac gggtccagag agcgcaacag ccctgggata gccccgccgt aaaaagcttg
     4081 ttcgggcacg accgggtccg cgaagttctc ttcaacggcc agataagcgc tggtgaactg
     4141 ctggaccgcg cggcgaagct cctcctcgtc gcctggagtg acggggcggc gctccggcgt
     4201 ggccggagtc tggggccgcg tggttccgga agt
//
LOCUS       DASREK010000271         5840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_800163, whole genome shotgun sequence.
ACCESSION   DASREK010000271 DASREK010000000
VERSION     DASREK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800163"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3290)
                     /locus_tag="VFA54_14675"
     CDS             complement(<1..3290)
                     /locus_tag="VFA54_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZS52108.1"
                     /translation="MAVTKIKGRYEIRQILGQGGMGVVYKAFDSLIKREVALKTIRDI
                     PDRTALDLFYKECDVLASMSHPNIVEIFDIGEFEEDGANKPYFVMPLLPGVTLDHLIR
                     TESHRLTVERSVDIMCQTCRGLQAAHDRGLVHRDLKPSNIFVMEDDSVKIIDFGVAHM
                     VDARSTMGHKGTLLYMAPEQIEMKPPSALSDIFSLGVVFYETFTRRRPFERSTTGEIA
                     QAILQQIPPPVSDLNPAVSQALSRVIHKAMAKQPWHRFSSARELAETLQKAQRNEPIE
                     FFDPARIQPRIQRATRAFEQADYQFAMEILSELEAEGHVDPTIALLRRQIDQATRQKT
                     IMQLLDSARTRFEEEEYPLALQKIQEVLQLDPNNAAALSLKSNVESKRSETKIDDWFR
                     LARQHIENHAFSHARQALQNVLQLKPRESRALQLLAEVDRKEQEYVRVRQEKQELYQA
                     SLEAWQNGEVSAALTKLERLLELDRRAPDTSAPERGAAYQNFYNQVRSEHDAIKNSYA
                     EARRLLEDGNFAQSLAICNEYLAKHPGNALFQALKFDVEERQRQGLSATIAQIDAKVE
                     AEPDLERKVNILKEALDQYPGEAHFERALRLMRDKRDLVNSIVAKARNHEERNQFTEA
                     LGQWEILKTIYAQYPGLNFEIERVIKRREQQSRAEAKVRWVEQIDRHLESADYSRAME
                     LLKEALLEFPDDAELTELQKLAGQGIERAAEAQALLGKGQELCNQGKIDEGIECLRRA
                     RQLDDRSPVIRAVLLDTLLEQARAVLDKDWRSAEQLTQQALELDPAHALAKSLRTLAW
                     DKKREEFVDQCVAQARQLQASGDIASALVKVQEGLSTYPRETRLTLLYGTLSKALPEG
                     ERPTGSHQAGPSQKPPASPPRFANDVEVTASEAGERPLHNYAEQYAETMLDPSARPAA
                     PERARLSLTPAPEPLATEAPAAGQNWAAPAEPAEPRPAPPDLERKKSVPEAPKRPATP
                     KPAAKAAAKAAKNIPAPPSSAKSKIWIPIAAASVLVLAVVGVVAGLRSRKAGVVAGTI
                     PVEIRTTPPGAAIRINNEARGSSNLKIELPEGSYEVEAQLDGYQPSVATIHPAAGAPA
                     TLDLALQPL"
     gene            complement(3706..4017)
                     /locus_tag="VFA54_14680"
     CDS             complement(3706..4017)
                     /locus_tag="VFA54_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52109.1"
                     /translation="MRALSAVAVTERRKKTRRFLEGLSTSELRYIASYLGACLLESAL
                     YSDQVSRRQIASDIAQYECRRCGSGCGFSGDVEHQMILLLEYLSACRRSVAVNVVAGS
                     A"
     gene            complement(4170..>5840)
                     /locus_tag="VFA54_14685"
     CDS             complement(4170..>5840)
                     /locus_tag="VFA54_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZS52110.1"
                     /translation="FHYTVPEPLFLPPVVIYGGFASGFALGEAGGRTAENCHNVGSAP
                     HSSMSIYAAIDIGSNSIRLAVADTESGRFYKLLAAERQVTRIGEGVFTSGRVAEKAIE
                     ETCAVLARFAATCQKWQPVAIRAVATSAIRDASNQAEFLARASKAAGVQIQVISGREE
                     ARLIHLGVLARWPKPDPEMLVLDIGGGSAEVIWSQSGEIRYSKSLPLGAVRLTESFLQ
                     HDPPAEEELAELHEYIQQQFAGTLRRLGRCAKARAIGTSATAAAAVCAVNRIRSSDRE
                     EAAGRRARYREIQALYRRLIRMDCRDRQRIPGIGPRRAEIIIPGVAVLQSFVEHFTGG
                     AIYYSPAGLRDGLIADLAARRVTSEAASLDRQQRRTVMEMARRYGVSLPHARQVATLA
                     RSLFLNLASAHRLPLSYARLLEAAAYLHDVGHYVSSIRHHKHSYYLVANSDMPGFTDR
                     EQLIVANICRYHRKALPSDEHDHFRTLKPDERRAVALLVPLLRVADGLDRGHRQLVTS
                     VEAEAQEGRICVFVKWRPGIELDLWAARRAAPSFGQVYRSELVISSQR"
BASE COUNT         1056 a         1850 c         1781 g         1153 t
ORIGIN      
        1 agcggctgca aagcaagatc gagggttgcc ggcgctcccg ctgcgggatg aatcgtcgcc
       61 acagaaggct gatatccgtc aagttgcgcc tcgacttcgt aagacccttc cggaagctca
      121 attttcaggt tcgacgagcc gcgagcttcg ttattgatac ggattgccgc cccgggcggc
      181 gtggtgcgaa tctcgaccgg gatggttccg gcgacgacac ccgccttccg ggagcgcagg
      241 ccggccacca cgcccactac cgccaagaca agtacggacg cagccgcaat cggaatccat
      301 attttcgatt tagcgctcga cggcggcgca gggatgtttt tcgccgcctt ggcagcagcc
      361 ttcgcggccg gtttgggcgt ggcagggcgc ttcggagcct cgggaacact cttctttcgc
      421 tccagatcag gcggcgccgg tcggggttcg gcaggttcgg ccggcgcggc ccaattctgt
      481 cccgctgcgg gcgcttctgt tgccagtggc tccggggcgg gggtgaggct caacctcgcc
      541 cgctcgggag cggccggcct tgcggaaggg tcgagcatcg tttccgcata ttgctccgca
      601 tagttgtgta acggacgctc cccagcctcg ctcgcggtga cctcgacatc attggcgaag
      661 cgaggtggag aggccggagg tttttgactc ggcccggcct ggtgggagcc cgtaggccgc
      721 tccccttcgg gcagagcctt gctcagcgtg ccgtatagca gcgtcagccg cgtctcacgc
      781 gggtaggtcg aaaggccttc ctgcactttc accagcgccg acgcgatatc gccgctggcc
      841 tgcaactgcc gggcctgcgc gacgcactga tctacaaatt cctcacgttt tttgtcccag
      901 gcgagcgtgc gcaggctctt ggccagagcg tgagccggat ccagttcgag cgcctgctgc
      961 gtcagttgtt ctgcggagcg ccagtccttg tcgaggacgg ctcgcgcctg ctcgagcaaa
     1021 gtgtcgagca ggacggcccg gatgacgggg cttcgatcgt ccagttggcg ggcccggcgc
     1081 aggcactcaa tgccttcatc gattttgccc tggttgcaga gttcctgtcc cttccccagc
     1141 agcgcttggg cttcggccgc ccgctcgatg ccttgacccg cgagcttctg caactcggtg
     1201 agctcggcgt cgtcaggaaa ctcgagcagg gcctctttca gcaattccat ggcccggctg
     1261 taatcggccg actccaagtg ccggtcaatc tgctccaccc accggacctt cgcctcggcc
     1321 cgcgattgct gctcgcgccg cttaatgacg cgctcgatct caaaattcag gccgggatac
     1381 tgggcgtaaa tcgttttcag aatttcccat tgccccaggg cctcggtgaa ctgattgcgc
     1441 tcctcatgat tgcgggcctt ggcgacgatc gaattcacca ggtcccgctt gtcgcgcatc
     1501 agccggagcg cacgctcgaa atgcgcctcc ccggggtatt ggtcgagggc ctctttcaaa
     1561 atgttgacct tccgctcgag gtcgggctcc gcctcgactt tggcgtcgat ctgggcgatg
     1621 gtggctgaaa gaccttgccg ctggcgctcc tcgacatcaa atttcagggc ctgaaacagg
     1681 gcgttaccgg gatgtttggc gaggtactca ttgcaaatgg ccagcgactg cgcgaagttc
     1741 ccgtcttcca gaagccgccg cgcctcggcg taggaatttt tgatggcgtc gtgttccgag
     1801 cggacctggt tgtagaaatt ctggtaagcg gcgccgcgtt ccggagcgga agtatcggga
     1861 gctcgccggt ccagctccag cagcctctcc agcttggtga gcgcggcgct gacttcgccg
     1921 ttctgccagg cctcgagcga ggcctggtaa agctcttgtt tttcctgccg cacccgcacg
     1981 tattcctgct ctttgcgatc cacctccgca agcaattgca gggcgcggct ctccctgggc
     2041 ttgagctgga gcacgttttg cagcgcctgc cgggcgtggc tgaaagcatg attttcgata
     2101 tgctgcctgg cgagccggaa ccaatcgtcg attttcgtct cgcttcgctt gctctcgacg
     2161 ttgcttttca gcgacaaagc ggcggcgttg ttcgggtcaa gctgaaggac ctcctggatc
     2221 ttctgcaacg ccagcgggta ctcctcctcc tcgaagcgcg tccgggcgct gtcgagcaac
     2281 tgcatgatgg tcttctgccg cgtcgcctga tcgatttgcc gccgcaaaag cgcgatggtc
     2341 gggtcgacat gcccttccgc ttcgagctcg ctgagaatct ccatggcaaa ctgataatcg
     2401 gcttgctcga aggccctcgt ggcgcgctgg atccgcggct ggatgcgcgc cggatcgaaa
     2461 aactcgatcg gctcgttgcg ctgcgccttt tgcagtgtct ccgccagttc gcgggcgctc
     2521 gagaaccggt gccacggctg cttcgccatg gccttgtgga tcacccggct caaggcctgg
     2581 cttacggcgg ggttcaaatc ggaaaccggc ggcggaatct gctggagaat cgcctgcgca
     2641 atctcgccgg tcgtcgaccg ctcgaaagga cgccgacggg taaaggtctc gtaaaacacg
     2701 acgccaagcg aaaaaatatc ggaaagcgcc gacggcggct tcatctcgat ctgctcgggc
     2761 gccatataca ggagagtccc tttgtgcccc atggtggagc gcgcatccac catgtgcgcc
     2821 acgccgaaat cgatgatctt caccgagtcg tcttccatca cgaagatgtt gctcggcttc
     2881 agatcccggt gcacgaggcc gcggtcgtgg gcggcctgaa ggccgcggca ggtttggcac
     2941 atgatatcga ccgagcgctc tacggtgagc cgatggcttt cggtgcggat caggtgatcc
     3001 agcgtcaccc cgggcagcag gggcatcaca aagtacggct tgttggcccc atcctcttcg
     3061 aattccccga tgtcaaagat ctcgacgatg ttcggatggc tcatggaagc cagcacatcg
     3121 cattctttgt aaaacagatc gagagccgtg cggtcgggaa tatcccggat ggttttcagg
     3181 gccacttccc gcttgatcaa ggaatcaaac gccttgtaaa ccacgcccat cccgccttgg
     3241 cccaggattt gcctgatttc gtagcggccc ttgattttcg ttacggccat ttgttccgct
     3301 tctccgcctg gactgttacc tgacgagggc ctggaaagcg atgcagcccc cttgaaacgg
     3361 gccggctccg gatcgcgacg cacgccgccc cagttctccc gcgcttctac cccttccacc
     3421 cagcgaggcg agtgtttaca tcgagcccgc aaggacgccc tgtggtcacc cttgcgcctt
     3481 cagatagctt tttcgatccc agcccctcca cttcgcaagg cttcgcttcc ggtcaaaagt
     3541 taattttaat agcagagcca ccggaagaaa accgaaaaac agcctttatt cgctggggca
     3601 tggaggaatt cgggccttgc ccgagcgcct tgcggcagtt cgtccagccc ggtgaagcgc
     3661 gccggcaatc aatatctggg aaccgattca aatttcgggg gtggatcagg ccgatcccgc
     3721 taccacgttg accgctaccg agcgccggca ggcgctgagg tactcgagca gcagaatcat
     3781 ctgatgctcg acgtcgccgg aaaacccgca gcctgacccg caccggcggc actcgtattg
     3841 agcaatatcg gaggcaattt ggcggcggga aacctgatcg gagtacaggg ccgattccag
     3901 taagcaagcg cccaggtagc tcgcgatata gcgcagctcg ctggtcgaaa gcccttctag
     3961 gaaccggcgc gtcttcttcc gccgctccgt cacggccaca gccgaaagcg ccctcactaa
     4021 agacgcccgt tcactggcaa ctggcaattg cagctcctgc ggctctccaa taactatgcg
     4081 gtatttagat tatggagcaa gacaaggcgc cggagcaaga tgtaaatttc ctcagagcac
     4141 cactgatcgg tagtgccctt acctgaaatt cagcgctgtg aactaatgac cagttccgaa
     4201 cgataaacct gaccgaaaga cggcgccgca cgccgggccg cccagagatc cagctcgatg
     4261 ccgggccgcc actttacgaa gacacagatc cgtccttcct gcgcctccgc ctctactgac
     4321 gtcacaagct ggcgatggcc gcgatcaagg ccatcggcga ctcgcaggag cggcacaagg
     4381 agcgcgacgg cgcgccgctc gtcgggcttc agcgtccgga aatggtcgtg ttcgtcgctg
     4441 ggaagcgcct tacggtgata gcggcagatg ttcgccacaa taagctgttc ccggtccgtg
     4501 aagccgggca tgtccgaatt cgccaccaga tagtaggagt gcttatggtg ccggatcgag
     4561 ctgacgtaat ggccgacgtc gtgcaaatag gcggcggcct cgagcaggcg ggcataagaa
     4621 aggggcagcc ggtgggccga ggccagattg aggaacagcg accgcgccag ggtcgcaacc
     4681 tgacgggcgt gcggcaggga aaccccgtaa cggcgcgcca tttccatcac cgtgcggcgc
     4741 tgttgcctgt ccaatgaggc cgcttccgag gtaacgcggc gcgccgccag atcggcgatg
     4801 aggccgtcac gcaagcccgc cggggaatag taaattgcgc cgccggtgaa atgctccacg
     4861 aaagattgca aaacagccac accggggatg atgatttccg cccgccgcgg gcctatgccg
     4921 ggaatgcgct gccggtcccg gcagtccatg cggatcagcc ggcgatacag cgcctgaatt
     4981 tcccggtatc gcgcccggcg tcccgcggct tcctcccggt cggaactgcg aatccggttc
     5041 acggcgcaga cggcggccgc ggccgtggcc gaggttccga tagcgcgcgc tttcgcgcag
     5101 cgcccgagcc ggcgcaacgt cccggcaaat tgctgctgta tgtattcgtg gagctcggca
     5161 agttcctctt ctgccggcgg atcatgctgg agaaagcttt ccgtcaaccg gaccgcgccc
     5221 agaggcagag atttgctgta tcggatctcg ccgctctgac tccagatgac ctcggcgctg
     5281 ccgccgccga tatcgagcac cagcatctca ggatccggct tgggccagcg ggcgaggacg
     5341 cccaagtgaa tcaaccgggc ttcttccctg ccggagatga cctggatctg caccccggcg
     5401 gccttggatg cgcgggcgag gaactcggcc tgattgctcg catcgcggat tgcgctggtc
     5461 gccacggcac ggatcgcgac cggctgccat ttctgacatg tcgccgcaaa cctcgctagc
     5521 acggcgcaag tctcctctat cgccttctcg gcaaccctgc cgctggtgaa cacgccttcg
     5581 ccgatgcggg tcacctgtct ctcggcggcc aacagcttgt agaagcggcc ggactcggta
     5641 tcggcgacgg ccagccgaat ggaattgctg ccgatatcaa tagcggcgta aatgctcatc
     5701 gaggaatgcg gcgcagaacc gacattatgg cagttttccg ccgtacgccc gccggcctct
     5761 cccaacgcga agccgcttgc aaagccgccg taaatgacga cgggcggcaa aaagagcggt
     5821 tccggcacgg tataatggaa
//
LOCUS       DASREK010000272        30010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2436359, whole genome shotgun sequence.
ACCESSION   DASREK010000272 DASREK010000000
VERSION     DASREK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 30010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30010
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2436359"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFA54_14690"
     CDS             complement(<1..383)
                     /locus_tag="VFA54_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52111.1"
                     /translation="MAAHRRWLAPLVYLAILAWVNIYICRELFFTEYAGHMNSMHGFW
                     MAMARLAPAGHWIKPTWWPFWYGGMPFEYTYAPLAPDLTAAYAKIAGCSVARAFHAIS
                     GFFYCLTPFALFLTAWRMTSSAGYA"
     gene            616..1374
                     /locus_tag="VFA54_14695"
     CDS             616..1374
                     /locus_tag="VFA54_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS52112.1"
                     /translation="MPAYNAAKTLHLTYAELPRDLIDLVILVDDASSDETAAIARELG
                     LELFVHNRNYGYGANQKTCYREALKAGADIVVMVHPDYQYDPKLLPEVIRPIQEGKAD
                     VALGSRLLGASPIQQGMPWWKYVSNRFLTWLENLVFGLHLSEYHTGYRAFRRTALELV
                     NFDMNSDKFIFDQEILAQMVELKMRFAEVPVPTRYFPEASSASFWQSSVYGCSILWLL
                     FRYLLHRSGIFRQRQFESLTLRYRDSAAVHGPTP"
     gene            1701..3701
                     /locus_tag="VFA54_14700"
     CDS             1701..3701
                     /locus_tag="VFA54_14700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZS52113.1"
                     /translation="MTLVRRLFFVVPLAVCALAAKRPVQPEDYYRIQEAVEAEVSPDG
                     RWVAYVVEKNDRKLGSYGQVFLLSTRDGGSRLISDPAASDSGIRWKRDSSAFGFLSTK
                     GGHTSIWMATVNGKKQLLTPADNTNHVLPSTGHRFAWSPDGRQLAYVAADPPDRRASS
                     DPIHITRYLYKFIAGLDDNRRIHIHILDIAAKRSRKLTEGPYYEHTIDWSPDGKEILF
                     ESNRGPLEELEFNYDIFAVDVASGKERRLTNTKSQEYQAAWSPDGKWVAYLGTKREIT
                     CSETTMEDTHVWTIPASGGMGQEVPGTLRLDRRCFRPRWSVGGDSIYFMAQDHGNTHI
                     YAVPAHSSGSARQITRGAGTVVSYSTSDEGQVVFAFRSPSSPGEVFSQAGENQAAQPL
                     TRLNETWLSEVEWSLPEEVNYRSVDGAAVQAWITPALPASAAGARAKYPLILTIHGGP
                     HAMQGPAFNFKAQAYAAQGYSSMMINYRGSTGYGEKFSEGTYQNNDGTEWEDVNAGAD
                     YAIAHKAYVDADRLGIEGASYGGQLTNWGITRTNRFKAAIPTAGITNFISYNFNSYYH
                     DYYRVEYGGYPWEGDNYRILWEHSPLAYVTRVRTPVLFIHGALDNDVPVNDSEQYYIA
                     LKENHVETEMLLYPREGHGLREPGHIVDSIQRSLAWYQKYLK"
     gene            3838..5520
                     /gene="murJ"
                     /locus_tag="VFA54_14705"
     CDS             3838..5520
                     /gene="murJ"
                     /locus_tag="VFA54_14705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZS52114.1"
                     /translation="MDSELPLPAVEAVRGKAESRHARIVRSAGVVSGAVFLSRITGVL
                     RDLVFARFFGAGFVYDAFLAAFRIPNLLRDLLAEGALSAAFVTVFSQYMTNKGDEEAF
                     ELSNRVATILTPALMLACVIGIIFAPQGVDLMFPGFAEIPGKKELTVTLTRIMMPFLL
                     FIALSSKAMGVLNAKGIFAIPALASAFFNLGSIAFGLFAGFVLGPRLGFSPIAGMAIG
                     TLIGGSLQYLVQWPSLRRIGLRYHFALSLRDPGVRQIFRLMGPAVIGTAAVQVNVVIN
                     SNFASEILDASGHVANGPVSWLGYAFRFMQLPIGLFGVAIASATLPAIARSAAENNIP
                     EFRSTLSASLGLVFLLSLPSAVGLALLSEPMIGLIFQRGRFTANDTHQTALALTCYSL
                     GLIGYSAIKVLTPAFYALNEVRVPMMASLASIATNYGLNWAFIRVLNWGHAGLAFSTS
                     LVATINFLVLIVFLRRKIHRLEGRRLARSFSRILVASGAMGAVCAASTFSMRHWLGTR
                     ALARLADLSLTIPLGILVLYGACRAMHVEELSTASDAILGKSRAVLKSRRAP"
     gene            5510..6286
                     /locus_tag="VFA54_14710"
     CDS             5510..6286
                     /locus_tag="VFA54_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52115.1"
                     /translation="MHPDLERLREIQDLDHRIAELTREISYLPAHISEIEGKLESHRR
                     RLEADRALLSANVKDRKRLDDDIKVFESKISRLRDQMSEAKTNEQYRAFQHEIEFAQQ
                     EIRKIEDLILDRMAEAEILDQNVKKAEAALKQEAQEVEKEKKAAEQRTDADRAELALA
                     EKRRAELAAGVTPKNLLIYERIRKLRGGVGVAAAREGRCTACNVILRLHYRQRVRTNQ
                     EVLTCEACGRILYYQPPEEPKEEAPKEVSNCRLQSAEPRP"
     gene            6253..7419
                     /locus_tag="VFA54_14715"
     CDS             6253..7419
                     /locus_tag="VFA54_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZS52116.1"
                     /translation="MQIAECGTAPLTELSEEERLFRDTVRRFAVEKIRPYTREMDEQA
                     KFRREIIDQFFQLGLMGIEIPEEYGGQGGSFFQAVLAVEALSTVDPSAGVIVDVQNTI
                     VNNAILRWGADGQKRKYLPRLAADTVGSYALSEAGSGSDAFALATRAEDRGDHFLITG
                     QKLWITNAAEAGIFLLFANANPDAGYRGITAFLIEREFPGFRVGKKEDKLGIRASSTC
                     ELILESCRVPRENVLGEVGKGYKVAIETLNEGRIAIGAQMLGLAEGALEHAMAYAKQR
                     KQFGKPIAEFQAVQFELARMATDIEAARLLVYNAARLKDSGARFVAEAAMAKYFASLV
                     AERTASRAIEIHGGVGFTKDYPVEKLYRDAKIGTIYEGTSNMQLATIAKQLLGQ"
     gene            7555..8142
                     /locus_tag="VFA54_14720"
     CDS             7555..8142
                     /locus_tag="VFA54_14720"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZS52117.1"
                     /translation="MKTIARWASQYILPFGAPGVFLLALADSGLIPIAEGVDVLLFAQ
                     VVRQPGRALLYAALATAGSLLGCIFLYYISRLAGEVALARRASPQRIERVRRQIEKYE
                     ALTLVLPAMVPLPLPMKLFVIASGVFRVHFARFVIAIAFARVVRYFGIALLAYHFGNQ
                     TWIFIRQHAAIAIAVTVCILGLFYWFSEHLARKEL"
     gene            complement(8210..9226)
                     /locus_tag="VFA54_14725"
     CDS             complement(8210..9226)
                     /locus_tag="VFA54_14725"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZS52118.1"
                     /translation="MDNLTHSLTGLLLARAGMRRAAPRGTLLLLLAANAPDVDVLAWW
                     RGPLAYLRFHRSLTHAAAMAPVMAVLAAAAVWFLAGPARREFRWGSACLAALAGVGSH
                     ILMDFTNTYGIRPWLPFSGKWYSWDISFIVDLWVWAALLLAAGLPALGRLINAEIGAR
                     PETGKAAAVAGLLFIVAWWGARDLLHKQAVATLSQHLYGFDPAATSQPGDRAQGPSPP
                     LRVAAFPTAANPFAWRGLIETESFYQVINVDVRRPLDPSAGKILYKPSLTAPLEAALQ
                     ARTAREFSAFARYQYAAVDRMEGGGYRVTLTDLRFQGERRDAFLCTIDLDENLKVTRE
                     SFSF"
     gene            complement(9233..10156)
                     /locus_tag="VFA54_14730"
     CDS             complement(9233..10156)
                     /locus_tag="VFA54_14730"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZS52119.1"
                     /translation="MRYRRAVLIYNPAARHVKAGVIESAAAALQGHVACLETIPTAGP
                     GSGVHIARQACASAADLIVACGGDGTINEIINGMAGAAAPLAVVPAGTANVFALETGL
                     PLDPNAAAGLLPDLVPRRIGLGLLRRDGPPPCLRYFLLMSGVGLDAHIVYRLNPRWKK
                     RFGEFAYWAAGLSEFGRRRDSFEVSLEGRPYECTFALASRSRLYGGGLQIARRSHLLD
                     ASLDIVLFHTRSRWRYARHLVEVLTGRLDRSKAVTFAEARRMEARILERAGVRIEVDG
                     EYAGRLPASIEVVEDALTLLLPAAYIRRGQG"
     gene            10183..12183
                     /gene="ligA"
                     /locus_tag="VFA54_14735"
     CDS             10183..12183
                     /gene="ligA"
                     /locus_tag="VFA54_14735"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZS52120.1"
                     /translation="MSAKQAGVEEEIASLRRELERHEYLYYVLDQPEISDAEYDAMMA
                     RLRKLEAEHPELITPDSPTQRVGGKPREGFVRVRHSSPMLSLDNAYNEGELSDFDRRV
                     RELSGLEKIEYVTELKLDGLSLALHYTDRKLSRAITRGDGAVGEDVTENARTIRSIPL
                     HLPAAAPAGLVEPRGEVILTRSEFERINRERQEQNLALFANPRNAAAGSLRVLDPSIT
                     ASRRLLFFAYQYLLDGRPAGERQWEDLETLSRLGFKVNQHRRLCRGLAEVKEFINEWE
                     TKRSTLPYETDGVVIKVNEIALQKRLGWTAKAPRWAIAYKYAAHQAVTTVLDIGVSVG
                     RTGVLTPGALFEPVNIGGVIVSRASLHNEDEIGRLGIQIGDRVLVERSGDVIPHVVRV
                     EEPGKERRPFVMPTHCPVCGTRVVREPGEVAWRCVNTDCPARLKESLLHFAARRVMNI
                     DGMGEALVNQLVDRGLVHSISDIYSLTKEQLLSLERMGEKSASKLLRNIDSSRQASLD
                     RVINALGIPMVGERTAQILARHFGSLDKLMNAPVEELQRAEEVGPRVAEAIHSFFAEP
                     RNRELVERLRRAGLRFEQEPARRGGPLEGMTFVITGALRSMSREEATERIEAAGGKVT
                     SSVSKKTRYVVAGADPGSKLEKARDLGVEVIDESTLLRMLSG"
     gene            complement(12266..13063)
                     /locus_tag="VFA54_14740"
     CDS             complement(12266..13063)
                     /locus_tag="VFA54_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HZS52121.1"
                     /translation="MKPATIKAGIPRAARCGLLEREERDEIVMSHLPLVRAIACRVRE
                     NLPVQVELDDLVHAGILGLFDAVQKYDPRKRVMFHLYAKHRIRGAILDSLRQLDWASR
                     DLRKRFKHIEAITQKLSCQLGRTPTESEMARELGVSLPRWRKLSLELHAAGLGASQSY
                     PNGPGNESWERFSPAETPQSEEEPPDRTCAREQLRQVLAAAIRGLPARYQRVVMLYYA
                     HGSTMKQIGNELGVNESRISQIHKAALEKLQAALHSRGIHTTDMLVE"
     gene            13623..15044
                     /locus_tag="VFA54_14745"
     CDS             13623..15044
                     /locus_tag="VFA54_14745"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS52122.1"
                     /translation="MSTTTWNRTGGQHRVLLVGPDSAHLEYLRRLLEVKGFRIIRARA
                     IPEACQKIVIVAPHLVLADLGEERQAAGSALLPLVECAQGQPAPPAVVIVSDQQGQDS
                     GTESLKQAGASFLSKPVQPSELLATLSLAFAAYDAALLEPEQQKAVARPDLPPDFILG
                     RSPAIQRVIEQIRLVAPKDTSVLITGETGTGKERVSRAIHFLSGRRKSPMVSVNCGGI
                     PASLLEDEFFGHVKGAFTDAHQARMGRFEQANRSTIFLDEIGDLPLELQPKLLRVLQE
                     REIHRVGGVEAIKLDVRVVAATNVDLWALVQEGRFREDLFYRINVFPIHLPPLRQRRE
                     DIPLFVSHFLEKFARRDKLPPKRLRPEAEAELMRRPWPGNVRELENAVEIAVIRSQDR
                     EEVDVSDFPPARPLLQSDFPAAAPEGDFKSLVRQFEKDLILRVLERTKGNKSQAAQIL
                     RLKRTTLLEKLKKLQDPAESSSQ"
     gene            15041..15964
                     /locus_tag="VFA54_14750"
     CDS             15041..15964
                     /locus_tag="VFA54_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZS52123.1"
                     /translation="MSVWLLLSPGFPATILSMPRFTLAFITDEFCQDFERAASTAVEL
                     GYEAVEVRSVWNKNVADLEPGEVARIRRIADKAGVRILSVASPVFKCTHPEGGPVDHR
                     FEQDAFHSAHQFADQPRILERSIQIAVELGAPIVRVFSFWRTMEPQRLFHHIVESLAE
                     AIQKASRQGRWIGLENEHACNIATAAEAAPVLHALQDKHFGLVWDPGNSYMAGEIPYP
                     DGYSLLPPGRILHVHAKDGVLNRERDRVLWGPFGEGQVDWKRQIAALARDGYHGAISL
                     ETHWAGPDGNKEEGSRICAVRLKEMVLGYAG"
     gene            complement(16021..16821)
                     /locus_tag="VFA54_14755"
     CDS             complement(16021..16821)
                     /locus_tag="VFA54_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS52124.1"
                     /translation="MKLLLAASLAVAFVGPAKPQLFPPNEAGVTMGHIHLNVRDVEAH
                     RKLWVEQFGAVPIERGRLQGVKIPGMLILFRRREPAGGSEGTVLDHFGLRVRNLPDVL
                     QRWRAAGLLVQREFKGTEGFPNAYVMAPDGVKLELQQDVSLSTPAVSHHLHYYVADYG
                     SIRDWYTKTFSLRPSHRGAHESADVPGMNLSFQPAKAPPGMGTKGRALDHIGFEVKNL
                     EAFCQKLEAGGVKFDVPYRKVPDVGMGIAFLTDPLGVSIELTEGLESF"
     gene            complement(16979..17197)
                     /locus_tag="VFA54_14760"
     CDS             complement(16979..17197)
                     /locus_tag="VFA54_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52125.1"
                     /translation="MHCCPAFDQLVERDMACVSPKHLLSRGRIINDIDSDYFLAFGER
                     PLSYVGINYCPFCGRVVSRELWNREKKK"
     gene            17388..18191
                     /locus_tag="VFA54_14765"
     CDS             17388..18191
                     /locus_tag="VFA54_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZS52126.1"
                     /translation="MNLRLYRRWLGASCALLLFGAPALPQWRQWRQMMAEHDENPPTP
                     NPDEETEFYFARLAYRNGPGARRWGQAWMTDSPAADRYFLQGVRRLTNIHARSREQYI
                     PPLDNALFDYPWIYAVEVGQWGLNEAEGARLREYLMRGGFLVVDDFHGTYEWAHFVEG
                     IKKVFPDRPIVEIELSDPVFHVLYDLDQKVQIPGIQYLRSGRTFERDGVEPHWRGIYD
                     DKGRLMVVINFDMDLGDAWEWADFPGYPEQWTALAYRFGINYVTYAMTH"
     gene            18261..20510
                     /locus_tag="VFA54_14770"
     CDS             18261..20510
                     /locus_tag="VFA54_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZS52127.1"
                     /translation="MFEFLFKYPRTVFQKGEFLFLSGWPLWLLAALIAVAAAALAWHV
                     RVHGQRFARTRRGVIWALESALMALLLVILWHPAIRVATLRPQQNVIAVLVDTSRSMA
                     LKESTQSRLDKVQAALSGGLLRDLGGRFQVRLYDFSSGLERVENPTQLAANGNATRLG
                     EALAGVLRETSSLPLGGVVVFSDGADNANGIDRNLMAEIRQRRIPIHTVGVGRTEIPY
                     DAEMTDVAVSSRALPGSRLIARVSIRQHGLSGQKARLSVREGSNILAARDITLNSAQT
                     LQTEEILFNSGDPGARKLTFAIDPLPGEEVTGNNYLTRVLQVVPGKRRILYVEGEPRW
                     EFKFIRRAAEDDQSIQLVTLLRTSANRFYRQNVDDDKMLADGFPATAAELFGYQALII
                     GTIEANFFTPAQQDLIKEFVNRRGGSVLFLGGRHSFSDGGWATSLLAEILPVRITPGG
                     NTFHRRPVKVELTQQGRESLICRLDEDPEKNEARWNKLPDLADYQTTGELKPGAVSLI
                     DAQLPGAKTIPMLVMQNYGRGRVLVFATGGSWRWRMRLEHTDTSHVTFWQQLLRSMVS
                     NTPGPVMLTSDRVIYSDEDRVHLRAEARTQTYEPADNATVVATVTGEDGKPSTLEFHP
                     SPDQEGVYEADLAAEKPGSYRVEVTAHRESEILGRESLVFHREDGVAENFHPEQNREL
                     LQKIAQQTGGKYWDLNDVSKLPSEITYSEAGITAREARDLWDMPAVFFLALGLLAAEW
                     LLRRKWGAV"
     gene            20513..21580
                     /locus_tag="VFA54_14775"
     CDS             20513..21580
                     /locus_tag="VFA54_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52128.1"
                     /translation="MALSTERRTNAGFHRRFPALLKDGAALREESGFGGGSAPEARPA
                     PRSFPELFGTLGSWLLVSLLVFCPAALRAQTGSTYFLIVGGLGGEPDYEQRFASYVSD
                     LEKICRGMAGDPDHVIALSGKAATREGIESAFQKLSARVQPADALGVFLVGHGTFDGT
                     AYKFNIPGPDLTGEDLGALMNRVKAGRQLLVVATSASGACADKLAGNNRVVITATRSG
                     TERNAVVFARYWVEALRDPAADTDKNETISAQEAFHYADEKVKRFYTDQKRLATEHAR
                     MEGKMANSFVLARLGASAAAASDPAKRKLLAERESIEQQIDALKYRKSSLPDAEYKKQ
                     LEALLLQLARVQDAIEAGTGK"
     gene            21661..23850
                     /locus_tag="VFA54_14780"
     CDS             21661..23850
                     /locus_tag="VFA54_14780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52129.1"
                     /translation="MYRFLLALVLLAGSSLAAKAAPLDACQDPADHGRKEEARRCYLT
                     ALRTNADPAVQAEATWKLGDKKRANELFRAAVAAHPKDAAVRVAWGRLFLETYNKQEA
                     STLFKEALQLDPQNADAHLGLALVAAEGFENAAVEEANQALKLRPGLTEAHTLLASLA
                     LEENDAARANQHLDRALATKGCPLEAYALKAAIDLLAGKPESEWTAKALAYNPSYGDV
                     YATPAHFFEITRRYKEAVDLYRRALALDPDLWSAHAALAVNLWRLGDETEARQHLELA
                     YKGDPYNAMTVNSLRLMDSVDKHFRTFKNPKVILKLHEKEAEVLRPYFEELVQKAIDT
                     YRVKYAFTPTQPIHVEVYPDHEDFAVRTMGMPGLGALGVTFGYVVAMDSPSGRPPGEF
                     HWGSTLWHELCHVFVLEKTHHTAPRWLSEGLSMYEEAHGGEGWGDRITPEIIRAIKDK
                     RLLPIAELDRGFIRPRYPSQVIVSYFQAGAICEMIAEKQGFPKLLAMLDAYEHGRTTE
                     QVVQQVLGLSPADFDKQFAEFLRPRTEKVVASFDEWRKLMETVVSLAKAKKYSEVMEP
                     ARRARDLYPDFVEAGNPYEILAEAHLASGDKAGAAQELERYRLAGGRSPRALKQLASL
                     DDELGNRQQAIHVLEQLLWIRPGDEELHSKLGQLLLAEKQPRLAVREFESLLAVKPVD
                     PAAAHFHLAEAYYQLKNREKTREHLLASLEAAPGYRPAQKLLLEINR"
     gene            23865..24893
                     /locus_tag="VFA54_14785"
     CDS             23865..24893
                     /locus_tag="VFA54_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS52130.1"
                     /translation="MSTTTSLSTIETTDYRELVNRFRDVQTRIREQVHRIIVGQEEVI
                     DHLLLTLWVGGHCLITGMPGTAKTLLVRTLASALGLKFKRIQFTPDLMPTDVTGTDII
                     EEDVTTGHRKWTFVAGPIFTNVMLADEINRTPPKTQAALLEAMQELSCTVRGQTYRLE
                     PPFFVLATQNPIELEGTYPLPEAQLDRFLFNIILDYLNVKDEMEVVGRNTSGEMASVE
                     ACTNAEEIIAFQRLVKMVPIAENVTRYAVELMRATRPREASAPEVIKKYVNYGASVRA
                     TQFLVLSAKARALSRGNYHVGYDDLRALVLPVLRHRVLTNFHAESDGITVDEILRQLL
                     DAFPAPRD"
     gene            24955..25848
                     /locus_tag="VFA54_14790"
     CDS             24955..25848
                     /locus_tag="VFA54_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZS52131.1"
                     /translation="MPDAQRLLDPKILARLSTIELVAKTVVEGFVAGLHRSPTFGFSQ
                     EFAEYRPYVQGDDPRFVDWNVYARTDRTYLKRYLGETNTALVLLFDASNSMGYRSTGV
                     SKIEYARFLAASLAYLALHQHDAVSLILFDEKVRRYIPPSTRPGQLHGVLHAIEHAEP
                     ATRTNIQQPFEHFQQFLTKRGLVAVISDFYEQPERIIKAVRPLCYHGNDMMLFHILDP
                     AELSPPLRDSALLEDMETGERMEVAPEYARGEYRKLIQSHIDNLRREAQGAGADYLLL
                     DTSKPLDLALKEFLQFRYKRM"
     gene            26140..28170
                     /locus_tag="VFA54_14795"
     CDS             26140..28170
                     /locus_tag="VFA54_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HZS52132.1"
                     /translation="MSFLSPWFLIAGLAAMVFPVWLHLLERQNPTRIPFSSLMFFAHN
                     TQRAVRRRHLKYLALLAARLSLILLLALLFARPVFHRSASAASERPRHRIVAIDTSLS
                     MAYGDRWSRAQSEAQSLINQVRPGDHAQIVAFGPGVRVMNDPTDDRAVLRSALASLKP
                     TASRNSYGELGQSLRTLSENNAFAVEVHVISDFQQSAMPGRFADLSLPTGATLEAHSV
                     AGSGPHPNWCVESIKGETHVFGDGRPRIEATIAGFETPAASRRVWLNVNGRQVASKTV
                     LVPESGRATVEFAGFEVPYGESRGEVHIDSADPLPADDVRLLPFQRAEPSPILFLHNP
                     GKTRELLYYKTALEASGQSMFTVQPATPVEAGNLQLDRFAFVVICDVPRLPANIEQRM
                     KSYLQTGGSALLFLGPAIALEGQMPIAPDNAARRVIEARYTARERERFQQVSQMDGTH
                     AALRDSKSFSGVKFFRYLRWEPPASSVLARLADGSPLIAEETIGLGRLLVFGSSIDNL
                     WNDLPVHPLFVPFVFQSARSLSDLEETASQATIDSMLELEKRRDPRSAVEVIDPSGRR
                     ALGLAAAVSSREFPLTRTGFYEIRRTGKVELIAVNPDGRESDLRPIASDVLALWKSTG
                     RADNAAASAATAPSTPAPRDVWRMILIVVLAAAVIESLLGNLHLGLRDEVGS"
     gene            28170..>30010
                     /locus_tag="VFA54_14800"
     CDS             28170..>30010
                     /locus_tag="VFA54_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52133.1"
                     /translation="MNSFAALEEYLQQVKKRWQATVATRGLGIAAAGALILTILCVLF
                     ANRFAFAPWSVVSGRTVLYLATGALIALALVRPLLELRRRNSRTSWVKRIANRHPAFG
                     ERIETFVDQKPADRQNPMLGLLAEDTLRLTAQAPPEQIASSGPILTFSGIAVTALAVL
                     IWLGVAGPGYLRYGTARLWGGWLRPTMSNLYQLAVEPGSTTIRRRADLLISARTIGFY
                     SPTAHLFAKYASSAKWEEAPMQRRPDDSGFEFQLAGVDESLRYYVVAGSVKSPEYDVK
                     VVEMPSVKKLRLTYHYPSWTGLADSTQEPGGDVRAVAGTEVDVEVETDRPLTSGALQV
                     DGQASIPLRSDANWSRGRLAVQKDGRYFVAALYNGEVVRLSDDFFIEAVPDQPPTIKV
                     VKPARDAQATSIEEVPAQFEAEDDFGLKSFDLHYSVNGGPEKIVNLRAAGGKQATADY
                     LFALESFSLVPGDVISYYAAARDAKVETKTDMYFIEVQPFEREYYQAQQMGGGGGAGG
                     EDESSQISRREKEIVAATWNLARDKSLDKQKAADAAKTLAGVQSKLRDQTNTLAERMK
                     RRQLANASGEFQAFVKNMEQASAAMGPAAEQLSAQKWEGALPPEQKAL"
BASE COUNT         5773 a         9303 c         9099 g         5835 t
ORIGIN      
        1 aacgcgtatc cggctgagct cgtcatccgc cacgctgtca ggaacagcgc aaacggagtg
       61 aggcagtaaa aaaaccccga tatcgcgtgg aatgcgcggg caacggagca gcccgcgatt
      121 ttggcatacg cggccgtcaa atcgggcgcc agcggggcat acgtgtactc gaaaggcata
      181 cccccgtacc aaaaaggcca ccaggtcggc ttgatccagt ggcccgcggg agccagacgg
      241 gccattgcca tccagaagcc gtgcatcgag ttcatgtgtc cggcgtactc ggtgaagaac
      301 aattcgcggc agatataaat gttgacccac gccaggatag cgagataaac gaggggcgca
      361 agccatctcc gatgggcggc catgcgaatg atacctgatt ctgacttgta cggcccgccg
      421 ggcgcaacac agatcttctc ggtcctccag acccgatcaa tcgaagcccg ccgccggttg
      481 gtaaccggtg gttctattgc cagcttccct gccccaggct taagctaggg cgtggaaacc
      541 gccacgcaga tgccaggtgc tcaggaaaca gccgccggaa agccaagccc ccgcccgcca
      601 aaagttgtgg tagtcatgcc ggcttataac gccgcaaaga cactgcacct gacctatgcg
      661 gaactgccgc gcgatctaat agacctggta atcctggtgg atgacgccag ctcggacgag
      721 acggccgcca tcgcccgtga gcttggcctc gagctgtttg tccacaaccg caattacggc
      781 tacggcgcca accagaagac gtgctatcgc gaggcgctga aagccggagc ggatatcgtg
      841 gtcatggttc accccgatta tcagtatgat ccgaagctgc tgcccgaggt gatccggccg
      901 attcaggagg gtaaggccga tgtcgctctg ggatcgcggc tgctgggagc aagcccgatc
      961 caacagggca tgccctggtg gaagtatgta tccaatcgat tcctcacgtg gctcgagaac
     1021 ctggttttcg gactgcatct ttccgagtat cacactggct atcgggcctt ccgccgtacg
     1081 gcgctcgagt tggtgaactt cgacatgaat tccgacaagt ttatcttcga tcaggaaatt
     1141 ctggcgcaga tggttgagtt gaaaatgcgg ttcgcggagg ttcccgtgcc cacgcgctac
     1201 tttccggaag catcgtccgc ttccttctgg caaagttcgg tctatggttg ttcgattttg
     1261 tggctgttgt tccgttacct gctccaccgc agcggcattt ttcgccagcg gcagtttgag
     1321 agcctgaccc ttcgatatcg cgacagcgcc gcggtccatg gccccacgcc ttgacgcccg
     1381 cttagcggat tccttggctg tcagaaaaag tacagaaatg cggcgtgttt ccgctgtgaa
     1441 gggtgcctcg cgccgccgtt gatgcgccgc gctttctgcc ggagacgttg cagtttccgt
     1501 gcgccaaacc gcgaattctc gccgccaagt cgtcgaaatc caaaattttg tcgcgcgccg
     1561 gaccaggccg caaaatgcgg cgccttccct ctgtgcggga gcagcttcca gccgaagctt
     1621 cgcgctgaga tgctcgggct tgcccctaat ttacccctgg cgtaggatga gtcgattctg
     1681 tgctaatcgg gagcgctgcc atgacacttg tccgacgtct ctttttcgtt gtgcccttgg
     1741 ccgtttgtgc cctggccgcc aagcggcctg tccagcctga agattattac cggattcagg
     1801 aagcggtcga agccgaggta tcgcccgatg gccgctgggt ggcctatgtt gtcgaaaaga
     1861 acgatcgcaa gctcggcagc tacggccagg tctttctcct ttccacgcgc gacggcggct
     1921 cccggctgat cagcgatcct gccgccagtg acagcggcat ccggtggaaa cgcgatagct
     1981 ccgccttcgg ctttctttcc accaaaggcg gccacacctc tatctggatg gcgacggtca
     2041 atggcaagaa gcagcttttg acaccggcgg ataacacgaa ccatgtgctg cccagcacgg
     2101 gccaccggtt cgcctggtcc ccagacggcc gccaactggc ctatgtcgca gccgacccgc
     2161 cggatcggcg ggcatcgtct gacccgattc acattactcg ctacctctat aaattcatcg
     2221 ccggcctcga cgacaaccgc cgcattcaca ttcacattct cgatatcgcc gcaaagcgct
     2281 cgcgcaagct gaccgaggga ccctattacg agcacaccat cgactggtcg cctgatggca
     2341 aagaaattct ctttgagtcg aaccgtggcc cgcttgagga gctcgaattc aactacgaca
     2401 tcttcgccgt ggatgtcgcc agcggcaaag agcgccgcct tacgaacacc aagagccagg
     2461 agtatcaggc cgcctggtcg cccgatggca agtgggtcgc gtatctcggc acgaaacgcg
     2521 aaatcacctg ttcggaaacc acgatggaag acacccacgt gtggacgatt cccgcttccg
     2581 gcggcatggg ccaggaggtt ccaggcactc tgcgtctcga ccgccggtgc ttccgccccc
     2641 gctggtcggt cggcggagat tcgatctact tcatggctca ggatcatgga aacacgcata
     2701 tctacgcagt tccggcgcat agctcgggat ccgcgcggca gatcacacgc ggcgccggaa
     2761 cggtcgtctc ttactccaca tcggacgagg gccaggtggt ttttgccttc cgctcgccgt
     2821 cttcgccggg cgaagtcttc tcacaagcgg gcgagaatca ggcggcgcag ccgctcactc
     2881 gcctgaatga gacatggctg tcggaagtgg aatggagcct gccggaagag gtgaactacc
     2941 gcagcgtgga cggcgccgcg gtgcaggcct ggattacgcc ggcgttgccc gcttccgcag
     3001 ccggagccag ggccaagtat cctctcatcc tgaccatcca tggcggcccg catgccatgc
     3061 aggggccggc cttcaacttc aaagcccagg cgtatgccgc ccaaggctac tcgagcatga
     3121 tgatcaatta tcgcgggagc acaggctacg gagaaaaatt ctctgaagga acctaccaga
     3181 acaacgacgg gaccgaatgg gaggacgtca atgccggcgc cgactatgca attgcgcaca
     3241 aagcgtacgt cgatgccgac cggctgggga tcgagggcgc cagctacggc ggccagctta
     3301 ccaattgggg catcacccgc accaaccgct tcaaggccgc cattcccacg gccggcatca
     3361 cgaatttcat cagctataac ttcaactctt attatcatga ttattatcgc gtggaatacg
     3421 gcggttatcc ctgggaggga gataattaca gaatcctctg ggaacactcg cccctcgctt
     3481 atgtcacgcg ggttaggacg ccggtgctgt tcattcacgg cgcgctcgat aatgatgtgc
     3541 cagtgaacga ttccgagcag tattacatcg ccctgaagga aaatcacgtt gaaaccgaaa
     3601 tgctgctgta cccgcgagag ggccacggct tgcgcgagcc tgggcacatc gtcgattcga
     3661 tccagcgcag tctggcatgg tatcaaaagt atctgaagtg actggaagcg gccggccccg
     3721 ccttccgggc cccggcggga gagccgccgg gggctgctta gacccttctt ttgccgcgcc
     3781 cgtgcggctt gcatcctctt ccggagagcc cggcgcgacc tgctagagtg aaagtctatg
     3841 gattccgaac tgccactgcc ggcggtagag gccgtccggg gaaaggctga gagcaggcat
     3901 gcccgcattg ttcgctcagc cggagtggtg agcggcgcgg tttttctcag ccgcatcaca
     3961 ggcgtgctcc gcgacctcgt ttttgcccgg ttttttggcg cggggttcgt ttacgacgcc
     4021 tttttggccg cctttcgcat tccgaacctc ctgcgggatc tgcttgccga gggcgcgctt
     4081 tcggctgctt tcgtcactgt gttttcgcaa tacatgacga acaagggcga tgaagaagcc
     4141 ttcgagctgt cgaaccgcgt cgctacgatc ttgactccgg cgctgatgct ggcctgcgtg
     4201 atcggcatca tcttcgcccc gcagggggtc gatctgatgt tccctggttt cgcagagatc
     4261 ccgggaaaaa aggaactcac ggtcaccctc acgcggatca tgatgccttt cctgctgttc
     4321 atcgctctct cctcgaaggc gatgggcgta ttaaacgcca agggcatttt cgccatcccg
     4381 gctttggctt ccgccttttt caacctggga tcgatcgctt ttggcctgtt cgcgggattt
     4441 gttctcgggc cgcggctggg cttcagcccc attgccggaa tggccattgg aaccttgatc
     4501 ggcggcagcc tgcaatatct ggtgcaatgg ccgagccttc gccgcatcgg tctccggtat
     4561 cacttcgccc taagtttgcg cgatcccggc gtacgccaga tttttcgcct gatggggccg
     4621 gcggtcatcg gcacggcggc cgtccaggtc aacgtcgtga tcaacagcaa tttcgcttcg
     4681 gaaatcttgg acgccagcgg gcacgtcgcc aacgggccgg tttcctggct gggttatgcg
     4741 ttccgattca tgcaattgcc gatcgggctg ttcggggtgg ccattgcttc ggcgaccttg
     4801 cccgccatcg cgcgcagcgc cgccgagaac aacatccccg agtttcgcag cacgctttcg
     4861 gcttccctcg gcctggtgtt tctgctttcg ctgccctccg ccgttggcct ggcgctgctg
     4921 tcggagccca tgatcggtct catctttcag cgcggccggt ttactgccaa cgacacgcac
     4981 caaacggccc tcgccctcac ctgctattct ctgggcctca tcggctattc ggcgatcaag
     5041 gttcttacgc cggcctttta cgcgttgaac gaagtccgcg tgccgatgat ggcgagcctg
     5101 gcctccatcg ccaccaacta tggcctgaac tgggctttca tccgcgtgct caattggggc
     5161 cacgcaggcc tggcgttttc cacctcgctg gtggccacga tcaattttct cgtgctgatt
     5221 gtttttttgc gccgcaagat tcaccgcctg gagggtcggc gcctggcccg gagtttcagc
     5281 cggattctcg tcgcctccgg agccatggga gcggtgtgcg cggcgagcac tttcagcatg
     5341 cggcactggc tgggaacgcg cgccttggcc cggctcgccg acctctcgct gacgattcct
     5401 ctgggcatac tggttcttta cggcgcttgc cgcgccatgc acgtggaaga actcagcaca
     5461 gcaagcgacg cgattctcgg aaaaagtcgc gctgtgctaa aatcacgtcg tgcaccctga
     5521 cctcgaacgg cttcgagaaa ttcaggacct cgatcaccgc atcgccgaac ttacccgcga
     5581 aatcagctac ctgccggcgc acatctccga gattgaaggc aagctggaga gtcaccgccg
     5641 ccgtttggaa gctgaccggg ctctgctgtc ggcgaacgtg aaagatcgaa aacgcctgga
     5701 cgatgacatc aaggtttttg agagcaagat ctcgcgcctc cgcgatcaga tgagcgaggc
     5761 aaagaccaac gagcagtacc gcgctttcca gcacgaaatc gagttcgccc agcaggagat
     5821 ccgcaaaatc gaggacctga ttctggaccg gatggccgaa gctgagatcc tcgatcaaaa
     5881 cgtaaaaaag gccgaggcgg ccctcaagca ggaagcgcaa gaagtggaaa aggagaaaaa
     5941 ggccgccgag cagcgaaccg acgccgaccg ggcggagctg gcgctcgccg agaagcggcg
     6001 cgcggagttg gctgccggcg tcacgcccaa gaaccttctc atctatgaac gcatccgcaa
     6061 gctgcgcggc ggggtagggg tggcggcggc ccgcgagggc cgctgcacgg cctgcaacgt
     6121 cattctccgc ctgcactacc ggcaacgcgt gcggaccaac caggaagtgc tcacctgcga
     6181 agcgtgcggc cgcattctct actaccagcc ccccgaggaa ccgaaggaag aggcgccgaa
     6241 ggaggtcagc aattgcagat tgcagagtgc ggaaccgcgc ccctgaccga gctcagcgag
     6301 gaagaacgcc tctttcgtga caccgtccgg cggttcgcgg tggagaagat ccgcccttac
     6361 acgcgggaga tggacgagca ggcgaagttc cgccgcgaga tcatcgacca gttcttccag
     6421 ttgggcttga tgggcattga aattccggaa gaatacggag ggcaaggcgg atcgttcttc
     6481 caggccgtgc tggcggtcga agcgctctcc acagtggacc cctcggccgg cgtgattgtg
     6541 gacgttcaga acacgatcgt caacaacgcc atcctccgct ggggtgccga cgggcagaag
     6601 cgaaaatacc tgccccggct ggccgccgac accgtgggtt cttacgcgct ttccgaggcc
     6661 ggctcgggca gcgacgcttt cgctctggca acccgcgccg aagaccgtgg cgatcacttc
     6721 ctgatcaccg gacaaaaact ctggatcacc aatgccgctg aggcaggtat ttttctgctg
     6781 ttcgccaacg ccaatccgga cgcgggatac aggggaatca ccgcgttcct gatcgaacgg
     6841 gagtttcctg gatttcgcgt cggcaaaaaa gaggataagc tgggcatccg ggcctcttcc
     6901 acctgcgagc tgattcttga gagctgccgc gtgccgcgcg agaatgtgct cggcgaagtg
     6961 ggaaagggct ataaggtggc catcgaaacg ctgaacgagg ggcgcatcgc catcggcgcg
     7021 cagatgctcg gcctggcgga aggcgcgctt gagcatgcga tggcgtacgc gaagcagcga
     7081 aagcagttcg ggaagcctat cgcggaattt caagccgtgc aattcgagct cgcgcgtatg
     7141 gctaccgata tcgaggcggc ccggctgttg gtttacaacg ccgcccggtt gaaggattcc
     7201 ggagcccgtt ttgttgccga agcggccatg gccaagtatt tcgcctcgct ggtcgccgag
     7261 cgcacggcat cgcgggccat cgagattcac gggggcgtag ggtttacaaa ggactatccg
     7321 gtcgagaagc tctaccgcga cgcgaagatc ggaaccattt acgaagggac cagcaacatg
     7381 caactggcga cgatcgccaa acagttgctg gggcaatagc tttcaaccga atcatcagtc
     7441 cttctcctcc cggcgggacc ggcgcgctgg gccggggaag agtcttcgct ggaagcggaa
     7501 caggaacgcc ggcgtctgct gacaattgtt aacgatggct ggcgcctcct tcttgtgaaa
     7561 accatcgccc gctgggcttc ccaatatatt ctccctttcg gcgccccagg cgtttttctt
     7621 ttggctctgg cagactcggg gctgatcccc atcgccgagg gggtggacgt gctgctgttc
     7681 gcgcaggtcg tccggcagcc ggggcgggcg ttgctctacg ccgctctggc cacggcgggg
     7741 tctctcctcg gctgtatatt tttgtactac atatcgcggc tggcgggcga agtggctctc
     7801 gcccggcggg cttcgccgca gcgcattgag cgggtgcggc ggcagataga gaaatatgaa
     7861 gcgctgacgc tggtgctgcc ggccatggtt cccctgcccc tgccaatgaa gctgttcgtc
     7921 attgcgtccg gcgttttccg ggttcacttc gcgcggttcg tcatcgccat cgcattcgcc
     7981 cgcgtggtcc ggtacttcgg catcgccctg ctcgcgtatc attttggcaa ccagacgtgg
     8041 atctttatcc ggcagcacgc cgcaatcgct attgctgtga cggtctgcat tctggggctc
     8101 ttttactggt tttcggagca cctggccaga aaggaacttt gattccggcc ggcgcctgcc
     8161 gggcttcccg gctgcgttct cacagggggg ctggcggcgc cagactcttc taaaacgaaa
     8221 agctttcgcg cgtgaccttc agattctcat ccagatcgat cgtgcaaagg aaggcatcgc
     8281 ggcgctcgcc ttgaaagcgt aaatcggtca gagtaacccg gtagcctccg ccttccatcc
     8341 ggtctacggc ggcgtactgg taacgcgcaa aggcgctgaa ttcccgggcg gtgcgggctt
     8401 gcaaagccgc ctccagggga gcggttaacg aaggcttgta aaggattttt cccgccgacg
     8461 ggtcgagcgg ccgccgcaca tcgacattta tcacctggta aaagctttcc gtttcgatca
     8521 gtccgcgcca ggcaaaagga tttgctgcgg tcggaaaagc ggccacgcgc aatggcgggg
     8581 atggcccctg cgctcgatcg ccgggttggg aagtcgctgc cgggtcgaaa ccatacaggt
     8641 gctgcgagag cgtggcgaca gcctgcttgt gcaggagatc gcgcgctccc caccaggcca
     8701 cgatgaacag caatccggcg acggcggctg cttttccggt ttccggccgc gccccgattt
     8761 ccgcattgat aaggcgtccg agcgccggca agcccgccgc cagcagcagg gccgcccaca
     8821 cccacaggtc cacgatgaac gaaatgtccc agctatacca tttcccgctg aacggcagcc
     8881 acggacggat tccatatgta ttggtgaaat ccataagaat atgggagccc acccctgcca
     8941 gcgcggccag gcaagcgctg ccccaccgga actcccgcct ggccggaccc gccaggaacc
     9001 aaacagcggc agccgccaga acggccatca cgggagccat ggcagcggca tgcgtcaggc
     9061 tgcgatggaa ccgcaaatag gccaacggtc cccgccacca cgcgaggaca tcgacatccg
     9121 gagcgttggc cgccagcagc aacaaaagag tcccccgagg cgccgcccgc cgcatcccgg
     9181 ctctggctag cagcagaccg gttagggaat gtgtgaggtt gtccatttac catcagcctt
     9241 ggccccggcg aatgtaagcg gccggcagca gcagcgtgag cgcatcctcc acaacttcga
     9301 tgctggccgg cagccgcccg gcgtactccc catctacttc gatgcgaacg ccggctcgct
     9361 cgaggatgcg cgcctccatc cgtctcgcct cggcgaaagt gacagccttc gaacgatcga
     9421 gcctgccggt gaggacctcc accagatggc gggcgtagcg ccagcgggag cgggtatgga
     9481 acagaacaat gtccagactt gcgtccagca gatgcgagcg gcgggcgatt tgcagtccgc
     9541 cgccatacag gcgggagcgg gacgccagag cgaacgtgca ctcgtagggg cgtccctcca
     9601 gcgagacttc aaacgaatcc ctgcggcggc caaactcaga caggccggcg gcccaatagg
     9661 cgaattcgcc gaagcgtttt ttccaccgag ggttcaacct gtagacgata tgcgcgtcca
     9721 atccgacgcc gctcatcagg agaaagtatc gcaagcaggg cggcggacca tccctccgca
     9781 gcaggccgag cccaatccgc cgcggcacaa gatcgggcag caaaccagcc gctgcattcg
     9841 ggtccaaagg aagcccggtt tccagggcga acacattcgc tgtacccgcc ggaaccaccg
     9901 cgagcggcgc cgctgcgcct gccatgccat tgatgatttc gttaatggtc ccatcacccc
     9961 cgcaggccac gatcaggtcg gccgcgctag cgcaagcctg ccgcgcgatg tgaacaccgg
    10021 aaccaggccc ggccgtaggt atcgtttcca aacaggccac atgcccctgc aatgccgcgg
    10081 ctgcgctctc aatgacgcct gccttcacat gccgggccgc cggattgtaa atgagaaccg
    10141 cccgccggta tcgcacagcc tgaagttcct ataataacgg ccatgagcgc gaaacaagcc
    10201 ggtgtggaag aagagatcgc gtccttgcgg cgtgaactgg agcgccacga gtacctttac
    10261 tatgtgctcg atcagccaga aatcagcgac gcggaatatg acgccatgat ggcccgcttg
    10321 cgcaagctcg aggccgaaca tcccgagttg atcacgcccg attcccccac ccagcgcgtc
    10381 ggcggcaagc ctcgtgaagg ttttgtgcgg gtccggcact cctcgcccat gctgagcctc
    10441 gacaacgcat ataacgaagg ggaactttcc gacttcgacc ggcgcgtgcg ggagctgtcc
    10501 ggcttggaga aaatcgagta cgtcaccgag ctcaagctgg acggcctttc gctcgccctc
    10561 cactatacag atcggaagct gtcgcgggcc attacccgcg gcgatggcgc tgtcggagag
    10621 gacgtgacgg aaaacgcccg caccattcgg tccataccgc tccacctgcc agcggcagcg
    10681 ccggccggcc tggtggagcc gcgcggcgag gtaatcctca cccgaagcga atttgagcgc
    10741 ataaaccggg agcgccagga acagaatctg gccctttttg ccaatccccg caatgccgcc
    10801 gctggttccc tccgcgtttt ggatccatcc atcaccgctt cgcgccggtt gcttttcttc
    10861 gcctatcagt acctgctgga cggccgtccg gccggagaac ggcagtggga ggatttggag
    10921 acgctctccc gtctggggtt caaggtcaac cagcatcggc ggctttgccg cggacttgct
    10981 gaggtcaagg agtttatcaa cgaatgggaa acaaagcgtt ccaccctccc ttatgaaacc
    11041 gacggagtgg tcatcaaggt caacgagatt gcgctgcaaa aacgcctcgg ctggacggcg
    11101 aaggctccgc gctgggcgat cgcttataaa tacgccgccc accaggccgt gaccacggtg
    11161 cttgatatcg gtgtcagcgt gggccgcacc ggcgtcctca cgccgggagc cttgttcgag
    11221 ccggtcaata tcggcggggt catagtaagc cgcgcttccc tccacaacga ggatgagatc
    11281 ggccgtctcg ggatccagat cggggatcgc gtgctggttg agcgcagcgg ggacgttata
    11341 ccgcacgtgg tgcgggtgga agagccgggc aaggaacggc ggccgttcgt catgcccaca
    11401 cattgcccgg tttgcggcac ccgcgtggtc cgcgagcccg gcgaagtggc ctggcggtgc
    11461 gttaacacgg actgtccggc gcggctcaag gagtcactgt tgcatttcgc cgcccgtcgc
    11521 gtcatgaaca ttgatgggat gggcgaggcc ctggtgaacc agctcgtcga tcgcggcctc
    11581 gtccacagca tctccgatat ttacagcctg accaaggagc aactgctgtc tttggaacgg
    11641 atgggggaaa aatccgcgtc caagctacta cgcaacatcg actcgtcgcg ccaggcgtcg
    11701 ctcgaccgcg tgatcaacgc cctgggtatc cctatggtgg gcgagcggac ggcccaaatc
    11761 ctggcgcgcc atttcggaag cctggacaag ctcatgaatg cgcccgtaga ggaactacag
    11821 cgcgccgaag aggtgggacc gcgcgtggcc gaagccattc acagcttttt tgccgagccc
    11881 cgcaaccgcg aactggtaga gcgcctgcgg cgcgccggcc tgcgtttcga gcaggagccc
    11941 gcccgccggg gaggcccgct ggagggcatg accttcgtca tcaccggcgc cttgcgttcc
    12001 atgagccgcg aggaggcgac cgaacggatc gaggcggcag gcggcaaagt aaccagttcc
    12061 gtcagcaaga agacccgcta tgtggtcgcc ggggccgatc ccggatcaaa actcgaaaag
    12121 gcgcgcgatc tcggcgtgga ggtgatcgac gaatccaccc tgctgcggat gctgagcggc
    12181 tgaggcctgc ccggccgttt ccccgtccga tgcggcgccg gccgaacgcc gcggcgcggg
    12241 ccggcccggc cgggcccaag cacgcttact ccaccaacat atccgtggtg tggatgccgc
    12301 ggctgtgcaa ggcggcttga agcttttcaa gggcggcctt atgtatttgc gatatcctac
    12361 tctcgttcac gcccagctcg ttgccgattt gtttcatcgt gctgccgtgc gcgtaataca
    12421 gcatcaccac gcgctgataa cgcgcgggca agccgcggat ggctgccgcc agcacttgcc
    12481 gcaactgctc gcgcgcacag gtacggtccg gcggttcttc ctcgctttgc ggcgtctcgg
    12541 caggcgagaa acgctcccag gactcattgc ccggcccgtt cggataactt tgactggccc
    12601 ccaatcccgc ggcgtgcagc tccagtgaga gcttccgcca gcggggaagt gagacgccca
    12661 gttcacgggc catttcactc tcggtgggag ttcgccccaa ctggcagctt aacttttgcg
    12721 ttatggcttc gatgtgcttg aagcgcttcc gcagatcgcg ggaagcccag tccaactgcc
    12781 gcaggctgtc aagaatcgcg cctcgtatgc ggtgtttggc atagagatga aacatcacgc
    12841 gcttgcgggg atcatacttc tgaaccgcat cgaacagccc caggattccc gcatgcacca
    12901 agtcgtcaag ctccacctga acgggcagat tctcccgcac gcggcacgcg atcgcgcgca
    12961 ccaagggcag gtggctcatt acaatctcat cacgctcttc gcgctccagc aagccgcagc
    13021 gggcagctcg cggaatgccg gctttgattg tggccggttt cataacaggc gctgcgcatg
    13081 acatcgtcgg ttccttcctc cgatttccgt tttgtgttga ggagccgcgg aacgccgctg
    13141 cgggatctcg cggagatcgc gctgcttccg tgcggggcga gcctgtgcaa agcagtctcc
    13201 atttctccgc ttccctgagg ctatttgttt tcaacagtgg accgccgtta tccgctgagc
    13261 accgtcaaaa tcttgacgga aagtgacaga aagactatgc ctcccgcgtc gcgatgtagc
    13321 gtggctgtac tagacctttt gtagtttccg cacccgttcc gggcgccgct cgtcccaaaa
    13381 cgggaaaggc ggttccattt tggttcagac ccataaacgc agaattcttc gacaaatcac
    13441 gtcgaaagtc ctcccaatgc agtttcaaaa cggaaacaga agcgcccaaa atgtttttgc
    13501 cgccgcggct ggcgggcttg caatgcattg gtttgcgtca agatgactgg gcgtcatttt
    13561 tttggcaagc tccgtgcaca ttggccagcg ccgtccagat gcgtactggc cggcgctaca
    13621 ccatgtccac tacgacttgg aaccgcaccg gaggacagca tcgcgttcta cttgtagggc
    13681 ctgatagcgc ccatctggag tatctccgcc gcctgctgga ggtcaaaggg tttcgcatca
    13741 tccgagcccg agcgatcccg gaggcctgtc aaaaaattgt cattgtcgcg ccccatttgg
    13801 tactcgccga cctgggggag gagcggcagg ctgccggctc cgctctgctg ccgttagtgg
    13861 agtgcgcgca gggccaaccg gcgcccccag cggtggtaat cgtgagcgac cagcaagggc
    13921 aggattcggg aactgaatcg ctcaagcagg cgggcgcgtc gtttttatcg aagccggtgc
    13981 aacccagcga actgctggcg acgctttcgc tcgcctttgc cgcctatgac gcggctttgc
    14041 tcgagccgga gcaacagaag gccgtagccc ggcccgattt gcctcccgat ttcattctcg
    14101 gccgaagccc tgccatccag agagtaatcg agcagatccg gctggtggct cccaaggaca
    14161 ccagtgttct cataaccggg gagaccggca ccgggaaaga gcgcgtttcc cgggcgatcc
    14221 attttctttc cggccgcaga aagtcgccca tggtctccgt gaactgcggc ggcataccgg
    14281 cgtctttgct ggaagacgaa ttcttcggtc acgtcaaggg agcgttcacc gatgctcatc
    14341 aggctcggat ggggcgcttt gagcaggcca accgcagcac gatttttctg gacgaaatcg
    14401 gcgacctgcc gctcgagctg caacccaaac tgctgcgcgt gctgcaagag cgcgagatcc
    14461 atcgcgtagg cggcgtcgag gctattaagc tggacgtccg cgttgtcgcg gcgaccaatg
    14521 tggacctctg ggccctggtg caggaagggc gctttcgcga ggatctcttt tatcgcatca
    14581 acgtgtttcc cattcacctg cctcctctcc ggcagcggcg cgaagatata ccgttgttcg
    14641 tgtctcactt tctggaaaaa tttgcccgcc gggacaagct gccgcctaag cgcctgcgcc
    14701 cggaagccga agcggagcta atgcggcggc cttggccggg caacgttcgc gagctggaaa
    14761 acgccgtcga aatcgcagtg attcgcagcc aggatcggga ggaagtcgat gtctccgatt
    14821 ttcctcccgc gcggccgctg ctgcaaagcg actttcccgc cgccgcccca gaaggcgatt
    14881 tcaagagttt ggtccgccag tttgaaaaag acctgatcct gcgggttctc gagcggacca
    14941 agggaaacaa aagccaggcc gctcaaatcc tgcgcctgaa acgcaccacc ttgctcgaaa
    15001 aattgaaaaa gcttcaggac ccggcggagt cctccagcca gtgagcgtct ggctgctgct
    15061 ctcgccgggc tttcccgcta caatcttaag catgccgcgc ttcacgcttg ccttcatcac
    15121 cgatgaattc tgccaggatt ttgagcgcgc cgcgtcgact gcggtcgaac tcgggtacga
    15181 agccgttgag gtgcgctcgg tgtggaacaa aaacgtcgcc gacctcgagc ctggcgaagt
    15241 cgcgcggatc cgaagaatcg cggataaagc cggcgttcgg attctttcgg tcgcctctcc
    15301 ggttttcaag tgcacccatc ctgagggcgg cccggtcgat caccgcttcg agcaagacgc
    15361 ctttcactcg gcgcaccagt tcgctgatca gccgcgcatt ctcgagcggt cgatccagat
    15421 cgccgtcgag ctcggcgccc cgatcgtacg cgtgttcagt ttctggcgca cgatggagcc
    15481 ccagcggcta ttccaccata tcgtcgagtc gctggccgaa gccatccaaa aagcgtcccg
    15541 gcaggggcga tggatcggcc tggaaaacga gcacgcctgc aatatcgcga cagccgcgga
    15601 ggcggcgccg gtgctgcacg ccctccagga caagcatttc gggctggtct gggaccccgg
    15661 caactcctat atggccggcg agattcccta ccccgacggc tactccctgc tcccgcccgg
    15721 gcgcatcctt catgtccatg ccaaggatgg cgtgctcaac cgcgagcgcg atcgcgtgct
    15781 ttgggggcca ttcggcgaag gccaggtgga ctggaagcgc cagatcgccg cgctggctcg
    15841 cgacggctat cacggggcca tcagcctcga aacgcactgg gcagggcctg atggcaacaa
    15901 agaggagggc agccgcatct gcgcggttcg tttgaaggaa atggtcctgg gttacgcggg
    15961 ctaggccgca attcggacgg ccgggcttcc gagagaaggc ttgccttggc ggagttcgcg
    16021 ctagaagctt tccaggcctt cagtcagctc gatcgaaact cctaaagggt ccgtcaggaa
    16081 tgcgatcccc attccgacgt ccgggacttt tcggtacggc acatcgaatt tcacgccgcc
    16141 tgcctccagc ttctggcaga acgcctcgag gttcttcacc tcgaaaccga tgtgatcgag
    16201 cgcccggccc tttgtgccca tgccgggggg cgccttggcg ggttggaaac tgaggttcat
    16261 ccccggcaca tcggcggatt catgcgcccc gcgatggctg gggcgcaatg aaaatgtttt
    16321 tgtgtaccag tcccggatgg atccgtagtc ggccacatag tagtgcagat ggtggcttac
    16381 ggcgggggtg ctcagcgaaa cgtcctgctg caactcaagt tttacgccgt ccggagccat
    16441 cacgtaggcg ttgggaaagc cctccgttcc cttgaattcc cgctgcacga ggagccccgc
    16501 ggctcgccag cgctgtagca catccggcag attgcgcact ctaaggccga agtgatcgag
    16561 caccgttcct tcgctgccgc ccgccggctc tcgccgccgg aagagaatca acattccggg
    16621 tatttttacg ccttgcagcc ggccccgctc gatcggaacc gccccgaact gctcgaccca
    16681 tagtttccgg tgcgcttcca cgtcgcgcac gttcaggtgg atatgtccca tcgttacgcc
    16741 ggcctcgttc ggcggaaaaa gctgcggttt tgccggcccc acaaaagcaa cggccagact
    16801 tgcggcaagc agcagtttca tggtgattca ctccggcgtg tagtatacac ggaggcgaga
    16861 ggacgccgga aatctgccgg ctttttcggc ttcagcggcg ggcctcgcag catgcgtgcg
    16921 ggattctctt ttactgtgcg gcctctgcta tcgcccggat ccgccggcgc gctcgcgatt
    16981 acttcttctt ttcccggttc cacagctcgc gcgagaccac tcgcccgcag aagggacagt
    17041 aattgatgcc cacgtagctg agcgggcgct ctccaaaggc gagaaagtaa tcgctatcga
    17101 tgtcgttgat gattcgaccg cgggatagca aatgcttcgg cgagacgcag gccatgtccc
    17161 gttccacaag ttggtcgaat gccgggcagc agtgcacggt ttgagtctag cgccttttgt
    17221 tcgccgcgcc tagcacagcg ccgaagccgg cgcggtcctc ggtttgcgcg gcaaacggcc
    17281 ggaaggtctg tacttccaga aggcgcggcg ccagggataa aatcctacaa aatcgcggcc
    17341 tactccctta tggtattctt ttccgtgagg tccgtcgcga ggctgccatg aacctacgcc
    17401 tgtaccgccg gtggctgggg gcgagttgtg cgttgcttct gtttggggcc ccggcccttc
    17461 cccagtggcg gcagtggcgg caaatgatgg ccgagcacga cgagaatccc ccgacaccga
    17521 accccgacga ggaaactgag ttctatttcg cccgccttgc ataccgaaat ggccccgggg
    17581 cgcgccggtg ggggcaggct tggatgacgg actccccggc cgcggaccgt tattttttgc
    17641 aaggcgtccg ccggctgacc aacattcatg cgcggtcccg cgagcaatac atcccgccgc
    17701 ttgataacgc gctcttcgat tatccgtgga tttatgcggt cgaggtcggc cagtggggat
    17761 tgaacgaggc cgaaggggcg cgcctgcgcg aatacctgat gcgcggcggt ttcctggtgg
    17821 tggatgactt tcatggcacc tacgaatggg cccattttgt ggaagggatc aaaaaggtgt
    17881 tcccggaccg ccccatcgtg gaaatagaac tctccgaccc ggtctttcac gttctctatg
    17941 atttggatca gaaggtgcaa atcccgggca tccaatacct gcgcagcggc cgcactttcg
    18001 aaagagatgg agtcgaaccg cactggcgtg gaatttacga tgacaagggc cgcctgatgg
    18061 tggtgatcaa ttttgacatg gacttgggag acgcctggga atgggccgat tttcccggat
    18121 acccggagca atggacggcg ctcgcctatc gtttcgggat caactacgtc acttacgcaa
    18181 tgacgcacta gctcagcgat cagctctcag ctttccggag gctgacgggt cggtttctga
    18241 actgacggcc ggcacctttc atgtttgaat ttcttttcaa gtatccgcgc acggtttttc
    18301 aaaagggaga gttcctcttc ctgagcggat ggcctctgtg gctgctggca gcgctcattg
    18361 cggtggccgc agcggctctg gcctggcatg tgcgcgtaca cgggcagagg tttgccagga
    18421 ctcggcgggg cgtgatttgg gccctggaat cggcgttgat ggcgctgctt ctggtcatcc
    18481 tttggcatcc ggccattcgc gtcgccaccc tgcgcccgca gcaaaacgtg attgccgtgc
    18541 tggtggacac ctcgcggagt atggcgttga aggagagcac gcagtcgcgg ttggacaaag
    18601 tgcaggcggc cctttccggc ggcctgttac gcgaccttgg cggccgtttt caggtccgcc
    18661 tctatgattt ttcttccggc ctggagagag tcgagaatcc aacccaactc gccgccaatg
    18721 gaaacgccac ccgcttgggc gaggccctgg ccggcgtgtt gcgcgagacc agcagtcttc
    18781 cgctgggagg cgtggttgtt ttttccgacg gcgcagacaa tgccaacggc attgaccgca
    18841 acttaatggc cgaaatacgg cagaggcgta tcccgatcca tacggtcggc gtcggccgca
    18901 ccgaaatccc gtatgacgcc gagatgaccg acgttgcggt aagttcgcga gcgctgcccg
    18961 ggtcgcgatt gatcgcccgc gtcagcatcc ggcagcacgg gctgagcggc cagaaggcgc
    19021 gactgtcggt tcgcgagggc agcaacatcc tggctgcccg ggatattacg ttaaactccg
    19081 cgcagaccct gcaaaccgag gaaattcttt tcaattccgg cgatccggga gcgcgcaagc
    19141 tgactttcgc cattgatccg ctgcccggcg aggaagtcac gggcaataat tatctgacgc
    19201 gcgtgttgca ggttgttccc ggcaagcggc gcatcctcta tgtcgaaggt gagcctcgct
    19261 gggaattcaa gttcatccgc cgcgccgccg aagacgacca gagcatccag ctcgtaacgc
    19321 tgctgcgcac ctcggccaat cggttttacc ggcagaatgt ggatgatgac aaaatgctgg
    19381 ccgacgggtt cccggccacc gccgccgaac tattcggcta ccaggccctg atcatcggca
    19441 ccattgaggc caatttcttc acgcccgccc agcaggatct cattaaggag ttcgtgaacc
    19501 gccgcggcgg ttcggtgctg tttctgggcg gccgccattc cttcagcgac ggaggctggg
    19561 cgacgtcttt gctggctgaa attctgccgg tgcgaatcac tcccggcggc aataccttcc
    19621 accgccggcc agtcaaggtc gaattgacgc agcaaggccg cgaatcgctg atttgccgtt
    19681 tggatgaaga tccggagaaa aacgaggctc gctggaacaa gctgcccgac cttgccgatt
    19741 atcagaccac cggcgagctg aagcccggcg ctgtttccct gatcgacgcc cagttgcccg
    19801 gcgcgaaaac cattcccatg ctggtcatgc agaactacgg ccggggccgc gtcctggtat
    19861 tcgccaccgg cgggagctgg cgctggcgca tgcggctgga acacaccgac accagccatg
    19921 taacattctg gcagcaattg ctgcgcagca tggtaagcaa cacgccgggt ccggtgatgc
    19981 tgacttccga ccgggttatc tattcggatg aggatcgcgt tcatctgcgg gccgaagctc
    20041 gcacccagac ctatgagccg gctgacaacg ccaccgtagt ggctaccgta accggcgagg
    20101 acgggaagcc ctccaccctt gagttccacc cgtctccgga tcaggaaggc gtgtacgagg
    20161 ctgatctggc tgcggagaaa ccgggatcgt accgtgtgga agtcacagct caccgcgaga
    20221 gcgaaatcct cgggcgtgaa tccctggtgt tccatcgcga agacggagtg gctgaaaact
    20281 tccaccccga acagaatcgg gagctgctgc aaaaaatcgc gcagcagacc ggcggtaaat
    20341 actgggacct gaacgacgtg agcaagctgc cgagcgaaat tacttactcg gaagccggca
    20401 ttaccgcccg tgaggctcgg gatctctggg atatgccggc cgtttttttc ctggctctcg
    20461 gattgctggc ggctgaatgg cttttacgca gaaagtgggg cgcggtttga aaatggcact
    20521 ttcaacagag cgccggacga atgcgggatt ccaccggcgt ttcccggcgc tgctaaagga
    20581 cggcgcggcg ctgcgtgagg aatccggctt cggcggcggc tccgcgccgg aagcccggcc
    20641 ggcgccccgc tcgttccccg aacttttcgg aacactcggc tcctggctgc tcgtttcgct
    20701 tctggtcttc tgccccgcgg ccctgcgggc gcagaccggc tccacctatt ttctgatagt
    20761 cggcggatta ggcggggagc cggattacga gcagcgcttc gccagctacg tcagcgacct
    20821 cgaaaagatc tgccgcggca tggcgggcga tcccgatcac gtcatcgcgc tctccggcaa
    20881 agcggccacg cgggaaggca tcgagagcgc attccaaaaa ctttctgcgc gtgtgcagcc
    20941 ggccgacgct ctgggtgttt ttcttgtcgg ccacggcaca tttgacggga ccgcctacaa
    21001 attcaacatc cccgggcccg atctcaccgg tgaggatctc ggcgcgctga tgaaccgcgt
    21061 aaaagccgga cgccagttgc ttgtggtggc caccagcgcg agcggcgcgt gcgccgataa
    21121 actggccggc aacaaccgcg tcgtcattac tgcgacgcgc agcggcacgg agcgcaacgc
    21181 cgtcgttttc gctcgctact gggtcgaggc cctgcgcgat ccggcggccg acaccgacaa
    21241 gaacgaaacc atttcggcgc aggaggcctt ccactacgcg gatgaaaaag tgaagcgctt
    21301 ttacacggac cagaagcgtc tggccaccga gcacgcgcgc atggaaggca agatggctaa
    21361 cagcttcgtc ctggcccggc tcggcgccag cgccgcggcg gcgagcgacc cggccaagcg
    21421 caagctgctg gcggagcggg aatccatcga gcagcagatt gacgcgctca agtaccgcaa
    21481 gtcgtcgctc cccgatgccg agtataagaa gcaattggaa gccttgctgc tccagttggc
    21541 cagggtgcag gacgccatcg aagcgggaac cggaaaatag gatgtactcg cgtttccaga
    21601 gcaaaagcga gtgtccggat gcagcgggga acggtgattc gttcgctggc aggtagcacc
    21661 atgtatcgat tccttttggc tctcgtttta ttggctggct cgtccctggc ggccaaagcc
    21721 gcgcccctgg acgcgtgcca ggatccggcg gatcacggcc gcaaggagga ggcgcgccgc
    21781 tgttacctca ccgctctgcg cacgaacgcc gatcccgctg tgcaggcgga agctacatgg
    21841 aaactcggag acaagaagcg ggctaacgag cttttccgcg cggcagtggc cgcgcacccc
    21901 aaggatgccg cggtccgtgt ggcctgggga cgcctgttcc tggaaacgta taacaagcag
    21961 gaggcctcga ccctcttcaa agaggcgttg caactcgatc cgcaaaacgc agacgcgcat
    22021 ctgggcctcg cgctggtcgc ggccgaggga tttgaaaacg ccgccgtgga agaggccaac
    22081 caggccctga agctgcgtcc cggtttgacg gaggcccata ccctgctggc gtcgctcgcc
    22141 ctcgaggaaa acgatgcggc gcgagccaac cagcatctgg accgcgcgct ggccactaaa
    22201 ggttgccctc tcgaagccta cgcgctgaaa gccgccatcg accttctcgc ggggaaaccg
    22261 gagagcgagt ggacggccaa ggccctcgct tacaatccgt cctacgggga tgtttatgcc
    22321 acgccagcgc acttttttga gatcacccgg cgatacaagg aggcggtcga tctttatcgc
    22381 cgcgccctgg cgctcgaccc ggatctttgg agcgcccatg ccgcactggc cgtaaacctg
    22441 tggcggctcg gcgacgagac tgaagcacgc cagcacctgg aattagcgta caagggtgat
    22501 ccctacaacg ccatgaccgt caactctctg cggctcatgg acagcgtcga caaacatttc
    22561 cggacgttca agaatcccaa agtaattttg aagctgcacg agaaagaggc cgaggtgctg
    22621 cggccttatt ttgaagaact cgtgcaaaag gcgattgaca catatcgcgt gaaatacgct
    22681 ttcacgccca cgcagccgat tcacgtggag gtctatcccg accacgagga tttcgccgtc
    22741 cggaccatgg gcatgcccgg cctgggagcg ctcggcgtga ccttcggtta tgtcgtggct
    22801 atggacagcc cttccggccg gcctcccggc gaattccatt ggggcagcac tctgtggcac
    22861 gagctgtgcc acgtctttgt cctcgaaaaa acccatcaca cggccccacg ctggttgagt
    22921 gagggacttt ccatgtatga ggaggcccac ggcggcgagg ggtggggcga ccgcataacc
    22981 ccggagatca tccgcgctat caaggacaaa aggctgctgc cgattgccga actcgatcgg
    23041 ggcttcattc ggccgcggta tccttctcag gttatcgttt cctacttcca ggccggggct
    23101 atctgcgaga tgatcgcgga aaaacagggg ttccccaagt tgctcgccat gctggacgcc
    23161 tacgagcatg gccggacgac ggaacaggtg gtgcagcagg tgctcgggct ctcgccagcc
    23221 gatttcgaca agcagtttgc cgagttcctg cgcccacgca cggaaaaggt ggtagcctct
    23281 ttcgatgagt ggcgcaagct gatggaaacc gttgtcagct tggccaaggc gaagaaatat
    23341 tcggaagtca tggaacccgc ccggcgcgcg cgcgatctgt atcccgattt tgtagaggcc
    23401 gggaaccctt acgaaatcct cgccgaggcg catcttgcct ccggggataa agccggcgcc
    23461 gcccaggagc tggagcgcta ccgtttggcc ggcgggcgca gtccgcgggc tttgaagcag
    23521 ctcgcttcgc tcgacgacga gctcggcaac cggcagcagg ccatccacgt gctcgagcag
    23581 ctgctatgga tccggcccgg cgatgaagag ttgcattcga aactcggcca actgcttctg
    23641 gcggagaagc aaccccgctt ggcggtgcgc gagtttgagt ccttgctggc agtcaagcct
    23701 gtggaccccg cagccgccca ctttcatttg gcggaagctt actaccagct caagaaccgc
    23761 gagaagaccc gcgaacatct gctggcttct ctcgaggcgg cccccggata ccgcccagcc
    23821 cagaaattgc ttttggagat caaccgttag atataccgca tcccatgtca accactacat
    23881 cgctttccac gatagaaacg accgattata gagagcttgt aaatcggttt cgcgacgtgc
    23941 aaacgcgcat ccgggagcag gtgcaccgga ttatcgtcgg ccaggaagag gtcatcgacc
    24001 acctgttgct gactctttgg gtgggcgggc attgcctcat cacgggtatg ccggggacgg
    24061 cgaaaacgct gctggtccgc accctggcat cggcgctcgg gctgaaattc aagcgcattc
    24121 agttcacgcc cgacctgatg cctaccgacg tcaccggaac tgacatcatc gaagaagacg
    24181 tcacaaccgg ccatcgcaaa tggacttttg tcgccggtcc catctttact aacgtcatgc
    24241 tggcggacga aattaaccgc acgcctccca aaacccaagc tgctttgctg gaagcaatgc
    24301 aggaactaag ctgtacggtg cgcgggcaaa cctaccggct cgagcctccg tttttcgttc
    24361 tggcgacgca gaatcccatc gagctcgaag gcacctatcc tctgcctgag gcgcagctag
    24421 atcgatttct tttcaacatc attctcgatt atttgaatgt taaagatgaa atggaagtcg
    24481 tcggccgcaa cacctccggt gaaatggcca gcgtggaggc ttgcaccaac gccgaagaga
    24541 tcatcgcctt ccagaggctg gtcaagatgg ttcccattgc ggaaaacgta acccgctatg
    24601 cggtcgaact gatgcgggcg acgcgcccgc gggaagcaag cgcgccggaa gtcatcaaga
    24661 agtatgtcaa ctatggcgcc agcgtgcggg ccacacaatt tctggttctc tccgctaaag
    24721 cccgggcgct cagccgcggc aattatcacg tgggttacga cgatttacgc gccctggtct
    24781 tgccggttct acggcaccgc gtactgacga actttcatgc ggagtccgac ggcatcacgg
    24841 tcgacgagat tctgcgccag ctcctcgacg ctttccccgc tcccagagac tgacccgtgg
    24901 ccggtcttct cgaggttttc gccggaaaag cacagggaca aaacgagagg aattatgccg
    24961 gacgcacaac gtctcctcga cccgaaaatt ttggctcgcc tctcgaccat cgagctggtc
    25021 gccaaaacgg tggtcgaggg ctttgtcgcg gggctgcacc gctctcccac cttcggcttc
    25081 agccaggaat tcgccgagta ccggccgtac gtgcaaggcg acgacccccg gtttgtggat
    25141 tggaatgtgt atgcgcggac agaccgcacg tacctgaagc gctatctcgg cgaaaccaac
    25201 accgcgctgg tcctattgtt tgatgccagc aactccatgg gctatcgttc gacgggcgtg
    25261 agcaagatcg agtacgcccg gtttctggcc gcgtccctgg cctacctggc gctgcaccag
    25321 cacgatgccg tcagcctgat tctgtttgat gaaaaggtcc gccgctacat cccgccttcc
    25381 acgcgccccg gacagttgca tggcgtgctg cacgcgatcg agcatgccga gccggccacg
    25441 cgcaccaaca tccagcagcc tttcgagcac ttccaacaat ttttaacgaa acgcggcctg
    25501 gtggcggtga tctcggactt ttacgagcaa cccgagcgca tcatcaaagc tgtgcgcccg
    25561 ctctgctatc acggcaacga catgatgttg tttcatatcc tcgatccggc tgagctttcc
    25621 cctccgctgc gggactccgc cctgcttgaa gacatggaaa ccggtgagcg tatggaggtg
    25681 gctcccgaat atgcgcgggg cgaataccga aagctgattc aatcccacat tgacaacctt
    25741 cgccgggagg cccagggcgc cggggccgac tacctgctgc tggacacgtc aaagccgctc
    25801 gacctcgcgc taaaggagtt tttgcagttc cggtacaagc gaatgtaggg ttttccggct
    25861 ggcagcgatc cggctgtgct ctaaccgccg ctcacgcggc caacagtcct attccccaac
    25921 acttgcaaaa aacttacaat tttctttctc actggctacc cggagggcgg tctaatatag
    25981 agagatggtg gcctgggcct gtgcagaaac gcgtagtccc tcctccggga agcgtgggcc
    26041 gaggctgcgg ggcgctcccg gcgggcagcc agttcccacc aaagggttgc ggcgattacg
    26101 agtggccgag aagcgtatac ttaaaagggc tgacattcta tgtcctttct gtctccctgg
    26161 tttttgatcg ccggcttggc ggccatggtc tttcccgtct ggctgcacct gctcgaacgc
    26221 cagaatccga cccgcatccc gttcagttcg ttgatgtttt tcgctcataa cacccagcgg
    26281 gccgtgcgcc gcaggcattt gaaatacctg gcgctgctgg ccgctcgcct gagcttgatc
    26341 ctgctgctgg ccctgctgtt tgctcggccc gtatttcacc gcagcgcgag cgccgcctcc
    26401 gagcgcccgc gacaccgcat cgtggccatc gacacttcgt tgagtatggc gtacggcgat
    26461 cgctggagcc gcgcccagag tgaagctcag tcgctcatca accaggtgcg gcccggcgac
    26521 cacgcgcaga ttgtcgcctt cggtccgggc gtgcgggtga tgaacgaccc gaccgacgac
    26581 cgcgccgtgc tgcgcagcgc tttagccagc ttgaagccga ctgcatcgcg aaactcctat
    26641 ggtgaactcg gccaatcatt gcggaccctc tcggagaaca acgcgttcgc ggtcgaggtc
    26701 cacgtgattt ctgatttcca gcaatcggcc atgccgggtc gcttcgccga cctcagcttg
    26761 ccgacgggcg cgaccctcga agctcacagc gtggcgggca gcggcccgca cccgaactgg
    26821 tgcgttgaga gcattaaagg tgaaacgcat gtcttcggcg acggccggcc gcgcattgag
    26881 gccaccatcg ccggcttcga aacgccagct gcgtcgcgcc gcgtgtggct taatgtaaac
    26941 ggccgccagg tcgcgtccaa aacagtgctg gttccggaat ccgggcgggc cacagtcgag
    27001 tttgcgggtt ttgaggtgcc ctacggcgag agtcgcgggg aggtgcatat tgattcggcc
    27061 gatccgttgc ccgccgacga cgtgcggctg ctccccttcc agcgcgcaga accgtcgccc
    27121 atcttatttc tgcacaaccc aggaaagacc cgtgagttgc tgtactacaa gactgcgctc
    27181 gaagcttccg gccagtcgat gttcaccgtg cagcccgcca cgcctgtgga ggctggaaat
    27241 ttacagcttg atcgttttgc cttcgttgtg atctgcgatg tgccccggtt gccggctaac
    27301 atcgagcaac gcatgaaatc ttacctgcaa actggtggat cagcgctgct ttttctggga
    27361 ccggccatcg ccctggaagg ccagatgccg attgcccccg acaacgcggc gcggcgggtg
    27421 atcgaggctc gctacaccgc gcgtgagcgg gagcgcttcc agcaagtgag ccagatggat
    27481 gggacccatg ccgctctgcg ggacagcaag agcttttctg gggtgaagtt tttccggtat
    27541 ttgcgatggg agccgccggc ttcctccgtg cttgcgcgtc ttgccgacgg gtcgcctctg
    27601 attgcggaag aaaccatagg tctgggacgg ctgctggtat tcggttcgtc cattgacaac
    27661 ctatggaatg acttgccggt gcatccgctg tttgtgccgt ttgtttttca gtcagcccgg
    27721 tcgctttccg atttagaaga gacggcctcc caggccacga tcgattcgat gctggaactg
    27781 gagaagcggc gcgatccgcg ttccgccgtc gaggtgatcg atccttccgg acggcgtgct
    27841 ttgggactgg cagcggccgt atcgagccgc gaattcccgc tcacccgtac tgggttttat
    27901 gagatccggc gaactggaaa ggtggagctc atcgcggtga acccggacgg gcgggagagc
    27961 gacttgcgtc ccatcgcttc cgatgttctg gcgctgtgga aatccacagg gcgcgccgac
    28021 aacgccgccg cgtcggctgc caccgcgccg tccactcccg ctccccgcga tgtctggcgc
    28081 atgattctga tagtggtatt ggctgcggcg gtgatagaat cgctactggg caatctgcac
    28141 ttgggtctgc gagacgaggt gggatcctga tgaattcctt tgccgctctc gaagagtacc
    28201 ttcagcaagt caagaaaaga tggcaggcta ctgtcgccac ccggggcttg ggtatcgcgg
    28261 cggccggcgc cctgattctg accattcttt gcgttctttt tgccaaccgc tttgcgttcg
    28321 ccccctggag tgtcgtgagc gggcggaccg tcctgtatct ggcgacgggc gcgctcattg
    28381 ccctggcgct ggtccggccg ctgcttgagt tgcgtcgccg caacagcagg acctcctggg
    28441 taaagcgaat tgccaaccgc catccggcct tcggagagcg catcgagacc ttcgtcgatc
    28501 agaagccggc cgaccggcaa aaccctatgc tgggtcttct ggcggaagat acgctgcgcc
    28561 tcaccgccca ggctccgccg gagcaaatcg cttcttccgg tcccattctg actttttcag
    28621 gcatcgctgt gaccgccctg gccgtcctga tttggctcgg ggtcgccggc cccggctatc
    28681 tccggtacgg tacagcccgc ttgtggggcg gctggctgag gcccacgatg tcgaatctgt
    28741 atcagctcgc agtcgagccc ggctcgacga cgatccgccg ccgggccgat ctgttgatca
    28801 gcgcgcgcac tatcggattt tattcgccca ccgcccacct gttcgccaaa tacgcgtcga
    28861 gcgccaaatg ggaagaggcg cccatgcagc gccgcccgga cgattcgggc ttcgaatttc
    28921 agctcgccgg cgtggatgaa tcgctgcgct actacgtcgt cgctgggagc gtaaaaagcc
    28981 ccgaatacga cgtgaaagtg gttgagatgc ccagcgtgaa gaagttgcgg ctgacttacc
    29041 actacccatc gtggacggga ttggccgact ctacgcaaga gccgggcggc gatgttcgcg
    29101 ccgtggccgg aaccgaggtg gacgtcgagg tcgagaccga ccggccgctg acctccggcg
    29161 ctttgcaggt ggatggacaa gcgagcattc ccctccgcag cgacgcgaac tggagccgcg
    29221 gccggctggc ggtgcaaaag gacggccgtt actttgtcgc cgcactgtac aacggcgaag
    29281 tggtgcggct cagcgacgac tttttcatcg aagcggtccc cgaccagcct cccaccatca
    29341 aggtcgtaaa gcctgcccgc gatgcccagg ccacttccat tgaggaagtg ccggcgcagt
    29401 tcgaggccga ggatgatttc ggcctgaaaa gcttcgacct gcattattca gtcaacggag
    29461 gtccggaaaa gatcgttaat ctgcgggccg caggcggaaa acaggcgacg gcggactacc
    29521 tctttgcgct ggagagcttt tcgctcgtac ccggggacgt tatcagctat tacgccgccg
    29581 cccgggacgc gaaggtcgag accaagacgg atatgtactt cattgaggta cagcccttcg
    29641 agcgggagta ttatcaagcc cagcagatgg gcggcggagg cggagccggc ggcgaggacg
    29701 agagttccca aatttcccgg cgcgagaagg agatcgtggc tgccacctgg aacctggctc
    29761 gcgacaagag cctggataaa caaaaggcgg ccgatgccgc caaaacgctg gccggggtcc
    29821 aatccaaatt gcgcgatcag accaatactt tggcggagcg catgaaaaga aggcagcttg
    29881 ccaatgccag cggcgagttt caggcgttcg tgaagaatat ggagcaggcg tctgcggcca
    29941 tgggtcccgc ggccgaacag ctttcggcgc agaagtggga aggcgcgctg cctcccgaac
    30001 agaaagctct
//
LOCUS       DASREK010000273         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2280390, whole genome shotgun sequence.
ACCESSION   DASREK010000273 DASREK010000000
VERSION     DASREK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280390"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="VFA54_14805"
     CDS             complement(<1..1793)
                     /locus_tag="VFA54_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HZS52134.1"
                     /translation="MKPITRAAVLGAGTMGSRIAAHLANAGIPTDLLDLPGPPEDVNR
                     LARTGLAAALEQEPPALFLAGYRDRIAIGNFDNDLARLERCDWIIEAVAEDLDIKRRL
                     LARVEPVRRGDGVVSSNTSGIPVAAIARNFSEPFQQHWLGAHFFNPPRYMHLVELIRG
                     PRTLPAVFDWISEICDWRLGKGVVESKDTPGFIANRIAAFSSAAALRLMQEYDFSIEE
                     VDALTGSAIGNPKTATFRTLDLVGLDLAAHVIRDLYNNLPADPRRDWFRIPHFVEQML
                     AKNWLGEKTGQGFYRTQMKEGRREIWAIDWKTLEYHPRQKPRFASVEIGRNIEDVRQR
                     LPLLLASGDKAAQFLWSLLSEVFLYAASLVGEICESVVEIDRAMRWGYGREVGPFEAW
                     DALGVESVAARLKREKKDVPESVGRMLAGGARSFYQFLDGAAPVRAYFHFRESRYKPV
                     PLRPGILLLEDLKRAQREIKCNAGASLIDLGDGVLCAEFHSKMNTIGEDQVSILMAGL
                     KETAANFEALVVANQGGNFSAGANLVLVLMAAQGGEWDELDAAVRRFQQMNLALKYAP
                     KPVVAAPFGLTLGGGCEIALHCARNQAAAEL"
     gene            complement(2066..>3563)
                     /locus_tag="VFA54_14810"
     CDS             complement(2066..>3563)
                     /locus_tag="VFA54_14810"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HZS52135.1"
                     /translation="DTAVFVNKNVGNGKTISVTGISISGADAGNYLLSNTTATTTANI
                     TPRPLTITAAGVNKLYDGTTTATVTLSDNRVAGDVFTDTYASANFIPDGSVGNGKTVN
                     VAGISISGSDAGNYTFNTTATTTASILARNTAISISLSTNPIGEGSSTMVTVTVSDIS
                     GGTLFTPSGSVTLASSYPTLDSFGACVLAQGANPVGVATCSVLMTPLDDIPAPSPANY
                     SGSTLHTITALYAGDSTHFGAGPATTGLTVNNIAPAVSTPAGPTNPLPVTAATANISA
                     NFTDAGVADTHTCLISWDDNSSSVGTVNETNGSGSCSGSHTYTAAGVYTVTVKVTDDD
                     GGTGTSQFQFVVVYDPSAGFVTGGGWIQSPIYSALPYMQVSGKANFGFVSKYQKGQST
                     PDGNTEFQFQAGNFNFHSTSYQWLVISGAKAQYKGNGIVNGSGNYGFLLTATDGSMSG
                     GGGVDKFRIKVWDVATGNIVYDNSYGSSDDIDSANPMAIGGGSIVIHK"
BASE COUNT          706 a         1100 c         1047 g          710 t
ORIGIN      
        1 tatagctcag cagccgcttg attgcgcgcg cagtgcaggg caatctcaca accgccgccc
       61 agggttaatc caaaaggcgc agccacgacc ggcttaggcg catatttcaa ggccagattc
      121 atctgctgga accggcgcac ggcggcatca agctcgtccc actctccgcc ctgggcggcc
      181 atgagcacga gcaccaggtt agcccctgcc gagaaatttc ctccttgatt ggccaccacc
      241 agcgcttcaa agttggcggc ggtctccttg aggcccgcca tgaggatcga cacctggtcc
      301 tcgccgatgg tgttcatctt ggaatggaac tcggcgcaca gcacgccatc ccccagatcg
      361 atcagggatg cccccgcatt gcacttgatc tcccgctgcg cccgcttgag gtcctccagg
      421 aggagaatgc cgggacggag agggaccggc ttgtaacggc tctcacgaaa atgaaagtac
      481 gcgcggactg gcgccgcccc atccaaaaat tggtaaaacg atcgagcgcc tcccgccagc
      541 atgcgcccca cactctcagg cacgtccttc ttttcgcgct tcagccgtgc ggctacgctt
      601 tccactccca aagcgtccca cgcctcgaac gggccgactt cgcgcccgta accccagcgc
      661 atcgcccgat cgatctccac gacgctttcg caaatttcgc ccacaagcga ggccgcatac
      721 agaaaaactt cgctcaaaag gctccatagg aattgcgccg ccttatcgcc ggaggccagc
      781 agcagaggca gccgctggcg cacatcctcg atatttctcc ctatttccac ggaagcgaac
      841 cggggcttct ggcggggatg atactcgagc gttttccaat caatggccca aatctcgcgc
      901 cggccctctt tcatttgcgt tcggtagaat ccctgccccg tcttctcgcc cagccagttc
      961 ttggcgagca tctgttcgac gaaatgcgga atgcgaaacc agtcccggcg cggatcggcg
     1021 ggcaagttgt tatataggtc gcgaatcacg tgcgccgcca ggtcgagccc caccagatcc
     1081 aatgtgcgga aggttgcagt cttgggatta ccgatggcgg agccagtaag cgcgtccact
     1141 tcctcgatgc tgaaatcgta ttcctgcatc agccggagtg cggcagccga ggaaaaggca
     1201 gctatccggt tggcgataaa gcccggcgta tccttggact cgaccacgcc tttccccagc
     1261 cgccagtcgc agatttcgga aatccagtca aacactgcgg gcagcgttcg gggaccgcga
     1321 attagttcca ccaggtgcat gtaacgcgga ggattaaaga aatgagcgcc cagccaatgc
     1381 tgctgaaagg gctcgctgaa gttccgggcg atcgcagcca caggaatgcc gctggtattc
     1441 gaggaaacta cgccgtcccc tctgcgcacc ggctcgaccc gcgccagcag ccgccgcttg
     1501 atgtccagat cctccgcaac cgcctcgata atccagtcgc agcgctccag ccgcgccaga
     1561 tcgttgtcaa aattcccaat cgcgatgcga tctcgataac ccgccaaaaa cagcgcgggc
     1621 ggctcctgtt cgagggctgc ggcgagaccg gtacgggcga gccggttgac atcctcgggc
     1681 ggcccgggaa ggtctaacag gtcggtagga atccccgcgt tcgccagatg ggccgcaatt
     1741 cgcgacccca tggtacctgc tccaagcacg gccgcgcggg tgatcggttt catggaggcc
     1801 ctgcggaaga gagttgagcc tgacttttcg actatagcag aacaggcagg ccgccggttg
     1861 ctccgcctgc tcccgcgcat gcgccatcga tgagactgga tagccctttc ggcttccggg
     1921 gtcgagcgct gcgcgggaaa cagctcgggc agtggcggaa gcgcgtcaga ctctggcgtc
     1981 cgccgccggg cggggctcgc tcgagaaagc gagattcagc ggcgccttgg aagaccgccc
     2041 catcccgcag caaggaaacc ttgccttatt tgtgaatcac gatgctgccc ccgccgatgg
     2101 ccatcgggtt tgccgaatcg atgtcgtcgg acgacccgta ggagttatcg taaacgatgt
     2161 tccccgtcgc aacatcccag accttgatgc ggaacttatc gactccgcct ccaccgctca
     2221 ttgagccatc agtggcggtc agcagaaagc cgtagttgcc ggagccgttg acgatgccgt
     2281 ttcccttgta ttgcgccttg gcgccggaaa taaccagcca ctggtacgag gtgctatgga
     2341 aattgaagtt tcccgcctgg aactggaact cggtattgcc gtccggcgtg gactggccct
     2401 tctggtattt ggacacaaac ccgaagttag ctttgccgct gacctgcatg tagggcagcg
     2461 cactgtagat cggagattgt atccacccgc cgccggtcac gaacccggcg ctcggatcgt
     2521 acacgaccac aaactggaac tggctggtcc cggttccgcc gtcgtcgtca gtaactttga
     2581 cggtgaccgt atacaccccc gccgctgtgt acgtgtgcga accggagcag gagccgctgc
     2641 cgttagtttc gttcacggtc cccacgctgc tgctgttgtc atcccaggag atcaaacagg
     2701 tatgggtgtc ggccacgccc gcatcggtaa aattggcgct gatgtttgcg gtggcagctg
     2761 taaccggcag tgggttggtc gggccggcgg gagtgctgac ggctggggct atgttgttca
     2821 cggtcaggcc cgttgtagcc ggtcctgcgc caaaatgcgt gctatcgcct gcataaaggg
     2881 ccgtgatcgt atgtagagtt gagccggaat aattggctgg actcggcgcc ggaatgtcat
     2941 ccagcggggt catcagtacc gagcaggtgg ccactccaac aggatttgcg ccttgcgcca
     3001 atacgcaggc gccgaaacta tccagcgtcg gatacgaaga tgcaagagtc acggagccgg
     3061 atggtgtgaa tagagttcct ccggagatgt cgctcaccgt gaccgtcacc atcgtactcg
     3121 atccctcccc gatcgggttg gtcgagagcg agatcgaaat cgcggtgttg cgcgccagaa
     3181 tgctggcagt cgttgtagcc gtggtgttga acgtatagtt gcctgcatcg gagccggaga
     3241 tggagatgcc ggctacgttt actgtcttgc cgttccccac gcttccatca gggataaaat
     3301 tggcggatgc gtacgtatct gtgaaaacat cccccgccac gcggttatcc gacagggtta
     3361 ccgtagcggt tgtggttccg tcataaagct tgttcacgcc cgcagccgta atcgttagcg
     3421 ggcgcggcgt gatattggcc gtggttgtgg ccgtggtgtt ggaaagcaag tagttgccgg
     3481 cgtcggcgcc ggaaatcgaa ataccggtca cgctgatcgt cttgccattc cctacgttct
     3541 tgttcacaaa cacagccgta tcg
//
LOCUS       DASREK010000274         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1290751, whole genome shotgun sequence.
ACCESSION   DASREK010000274 DASREK010000000
VERSION     DASREK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290751"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFA54_14815"
     CDS             complement(<1..296)
                     /locus_tag="VFA54_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52136.1"
                     /translation="MDSGLYSNILVVRLGSMGDILHMLPAVATLKRGFPRARITWVVE
                     AHWSVLLRGNPHIDCVFPLEMNLWRRQWRKPESWNRLRLACRELRAARFDLAMD"
     gene            complement(298..696)
                     /locus_tag="VFA54_14820"
     CDS             complement(298..696)
                     /locus_tag="VFA54_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52137.1"
                     /translation="MDTRTKIIDVDEAAARASGFRSNRRDVILAAGSFDVLQVPHARF
                     LTGLKSDGAVLVVAVYDDASLCRMRKQSKPVLNERARAQLVAALRAVDQVVIWPGDSL
                     DGLIARLRPKGVEFVPDGRNIIGEILDRHK"
     gene            complement(671..1747)
                     /gene="waaF"
                     /locus_tag="VFA54_14825"
     CDS             complement(671..1747)
                     /gene="waaF"
                     /locus_tag="VFA54_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HZS52138.1"
                     /translation="MKFLVRAANWVGDAVMSIPALRTIRERYPRDEIAVLARPWVAEI
                     YQREDFADRVILYENSGRHAGAAGRLRLGAELRAQRFDCAILFQNAFDAALLAWLARI
                     PKRIGYSRDGRGFLLTDPIAPPVAGAIPRHERYYYLELLRRAGLIKDLPECPEIRLRG
                     DAAAGRRLFSSLGLCPERILGVSPGAAFGSAKRWLPERFAAVAARLSAEWGAGVAVFG
                     TASEFELCQRVAEQIGPEARNFAGRTSLTEFIDLAAACLAFVTNDSGAMHIAAAVGVP
                     TVAVFGATDPEATGPASFLARVVRTPVDCSPCLLRECPIDHRCMTRVEAATVEQEASR
                     LVETVLKAQAGNQAFRNGHANQNH"
     gene            complement(1744..4101)
                     /gene="lpxK"
                     /locus_tag="VFA54_14830"
     CDS             complement(1744..4101)
                     /gene="lpxK"
                     /locus_tag="VFA54_14830"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZS52139.1"
                     /translation="MLFVYNLALALASIVLVPFYLLRGRRGGKYRGTLRQRLGWFGSS
                     LRETDGRAIWLHAVSVGEVLSCQELVAGLRRRLPYARILISTTTKTGQAMAREKLGGI
                     ADDFFYAPVDVPFAIRNTLRRVRPRLLIIAETEIWPNLFHQAKRFGASLLLVNARISD
                     RSAPRYRKLRFFFRRVLRQPDAILAQSPLDRQRLIEAGAPLETVEVGGNLKFDFRSAG
                     VKIAAELESLLERSGAGPVILAGSTREGEEEQVVEGFRQAAQKHRNALLIVAPRHPER
                     FDAVAEALSKSGFGFLRRSRLPQGAALSLPGILLLDSLGELASLYQVADAVFVGGSLV
                     PWGGHNVLEPAFAGRPIVTGPHMQNFRAIAGALLAAEAMIQVNDAQELGTALLRLIDR
                     PSEASALGTRARRLAESHRGAAERAAERAVELYHSAIPNDPPGWLGRALLGAPAVVFG
                     AAATLRLRAYKKGWKPRRRLDTFTVSIGNLTAGGTGKTPVTQWLIEQIETRGLRCAVL
                     TRGYRRSSPEAMMVLGPDEPALASRTGDEAQIIRKRFAIPMGIGADRYRTGKEIERRF
                     HPEVFLLDDGFQHVQLERDLDIVLIDVMAPFGRGALLPLGRLREPPESLARAGVLLLT
                     RAEPWERWEGLRQQLRRYNPHAPISFVRIEPAAWVDAASGEERPLSHLDSMPVLAFCG
                     VANPDSFWRAVAQTGARPAARVCYRDHHRYSAADVRRLSARARQAGAAALVTTEKDVI
                     NLAPGRNSWPAPLYWLKIRVSVEDAGRLLDEILNRALPARERAAR"
     gene            4145..>4734
                     /locus_tag="VFA54_14835"
     CDS             4145..>4734
                     /locus_tag="VFA54_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52140.1"
                     /translation="MKVLGKAITLAVLTAGMIPLVSPAAAEKTRFEPQKADEYPAHQT
                     SSNVTVAADPYESGPKVKDAFGKLNPAKLGILPVLLVIQNDSDTPLQLDRMRVQWITA
                     DRQRFDSVSAEDAQRSGPIRRPEVNPRPSPIPGIGRGSRRPKDEKDLAALEFVAPVVM
                     PHSEAHGFFYFPAGKERGRIAGSKIYITGIRDARSGR"
BASE COUNT          930 a         1580 c         1460 g          764 t
ORIGIN      
        1 aagtccatcg ccaggtcgaa tcgggccgct ctcaactcgc ggcaggcaag acgcagccgg
       61 ttccagctct ccggctttcg ccactgccgc cgccagagat tcatctcgag cgggaacacg
      121 caatcgatgt ggggattgcc gcgcagcagc acagaccaat gggcttccac cacccaggtg
      181 attcgagctc gcgggaagcc ccgcttcaag gtcgccacgg ccggcagcat gtgcaggatg
      241 tcgcccatgg aacccaggcg aaccaccaga atgttcgagt agaggccgga gtccatccta
      301 tttgtggcga tctagtatct ccccgattat attgcgcccg tccgggacaa attcgacgcc
      361 cttaggccgc aggcgagcga tcagcccgtc cagagaatcg ccgggccaaa tgacaacctg
      421 atccactgct cgcagggcgg ccaccagttg agcgcgcgcc cgctcgttca gcacaggctt
      481 ggattgcttt ctcatgcggc agagactcgc atcatcatat accgctacga ccagcacggc
      541 tccgtccgat ttcaacccag tcagaaagcg ggcgtgcgga acttgcagga catcaaagct
      601 cccggcggcg agaattacgt cgcggcggtt ggaccgaaaa ccggaggccc gggcggcggc
      661 ttcatcaacg tcaatgattt tggttcgcgt gtccatttct gaaagcctga tttcccgcct
      721 gcgccttgag gaccgtttcg accagtctgc ttgcctcctg ctctacagtg gctgcttcca
      781 cgcgcgtcat gcagcgatgg tcaatggggc actcgcgcag aaggcagggg ctgcaatcga
      841 ccggcgtccg cacgacgcga gccaggaaag acgccgggcc ggtcgcctcg ggatcggttg
      901 cgccgaatac agcgacggtc gggacgccca ccgccgcagc gatatgcatg gcgccgctgt
      961 cattggtcac aaacgccagg caggccgcgg caaggtcgat gaattccgtc aaactggtgc
     1021 gcccggcgaa attcctcgcc tccggtccga tttgctcagc gactctttgg cacaactcga
     1081 actccgaggc tgttccaaat acggccacac cggcccccca ctcggcggag agccgagccg
     1141 caacggcggc aaaccgttct ggaagccacc gtttcgccga gccaaaagcc gcgccaggac
     1201 ttacgcccag gattcgttcc gggcacagcc cgagactcga aaacaaccgc cggccggcgg
     1261 ccgcatcccc gcgcagccgg atctcggggc attcaggcag atccttgatc aatcccgccc
     1321 ggcgcaacag ttccagatag taataccgct catgccgtgg aatggccccg gcgacggggg
     1381 gcgcgatcgg atccgttaac agaaagcctc ggccgtcgcg cgagtagccg atgcgcttgg
     1441 ggatgcgggc cagccatgcc aatagcgccg catcgaaggc attctgaaac aagatagcgc
     1501 aatcgaaacg ctgcgcgcgc agttcggcgc caagccgcag gcgcccggca gcgccggcgt
     1561 ggcgtccgga attttcatac aggatcacgc ggtcggcgaa atcttcgcgc tggtaaatct
     1621 ccgctaccca cgggcgcgcc aggacggcga tttcatcgcg cggatagcgc tcgcggatgg
     1681 tgcgcagcgc cgggatcgac atcacggcgt caccgaccca gttggcggct cgcaccagga
     1741 atttcatcgc gcagccctct cacgcgccgg cagagcgcga ttgagaatct cgtcgagcaa
     1801 tcgtccggcg tcctccacgc ttacccggat cttcagccaa tagagcggcg ccggccacga
     1861 attccgtccc ggcgcgaggt ttatcacgtc tttctcggtg gtcaccaaag cagccgcgcc
     1921 agcctgccgg gcgcgggcgc tcaggcgccg cacatcggcg gccgagtacc ggtgatgatc
     1981 gcgataacag accctggccg ccggccgcgc gcccgtctgc gccactgcgc gccagaaaga
     2041 atccgggttc gccacgccgc aaaacgcaag caccggcatc gaatcgagat gagaaagcgg
     2101 cctttcttcc ccgctcgcgg cgtccaccca ggcagctggc tcgattcgga cgaaagaaat
     2161 cggcgcatgc ggattgtatc gccgcaactg ctggcgcaag ccttcccagc gctcccaggg
     2221 ctcggcgcgc gtgagcagca gcacgccggc gcgtgccagc gactcgggcg gctcgcgcag
     2281 ccgcccgagc gggagcagcg cgccgcggcc gaaaggagcc atgacgtcga tcaggacgat
     2341 gtccaggtcg cgctcgagct gcacatgctg gaacccgtcg tccagcagaa acacctcggg
     2401 atggaaccgg cgttcgattt cctttccggt ccggtagcgg tccgctccaa tgcccatggg
     2461 gatggcgaag cgcttccgga ttatctgcgc ctcatcgcct gtccggctcg ccagggcggg
     2521 ctcgtccgga ccgagaacca tcatggcttc cggtgacgat cgcctgtagc cgcgggtaag
     2581 cacggcacag cggagccccc gcgtttcgat ctgttcgatc agccattgcg taaccggcgt
     2641 tttgccagtg ccgcccgccg tcaggttgcc gatgctaaca gtgaacgtat ccagtcgccg
     2701 gcgcggcttc cagcccttct tataagcccg cagccggagc gtggcagcgg cgccgaacac
     2761 cactgccggc gcgcccagga gcgctcttcc cagccatccg ggaggatcgt tgggaatggc
     2821 agagtgatac agctccacag cgcgctcggc cgcacgttcg gccgcgcctc gatgagattc
     2881 ggccaagcgg cgcgcccgcg tcccgagcgc cgacgcctcg ctgggacgat cgattagtcg
     2941 cagcagggca gttcccaact cctgggcgtc gttcacctgg atcatcgcct ctgcggccag
     3001 aagcgcaccg gcgatggcgc ggaaattctg catatgaggg ccggtcacga tcggacgccc
     3061 ggcgaaggcg ggctcgagga cattgtggcc tccccagggg acgagagatc cgccgacgaa
     3121 caccgcgtcc gcaacctggt agagcgacgc cagttcgccc aaactatcca ggagcagaat
     3181 gcccggcaag ctcagcgccg ctccctgtgg aaggcgcgat cgccggagaa acccaaagcc
     3241 cgacttcgag agcgcctcgg cgacggcatc gaagcgctcc gggtggcgcg gcgccacgat
     3301 gagcagcgcg ttccggtgtt tttgagccgc ctgccggaag ccctctacga cctgttcctc
     3361 ctccccctcc cgcgtgctgc ccgccaaaat taccgggccg gcgccgctgc gctccagcag
     3421 agactcgagt tcggcagcaa tcttcacacc ggcggagcga aaatcgaact tgagatttcc
     3481 tcccacttct actgtttcca gcggggcgcc agcctcgatc aggcgctgcc ggtcgagcgg
     3541 agattgggcc agtatggcgt ccggctggcg cagcacacgg cggaaaaaga atcgcagctt
     3601 gcggtagcgc ggcgccgagc gatccgaaat gcgggcgttc actaacagga gactcgcccc
     3661 gaagcgcttc gcctgatgaa acaggttcgg ccatatctcg gtttcggcaa tgatcagaag
     3721 gcggggcctc acccgccgca gcgtgttgcg aatggcaaag gggacatcga ccggcgcata
     3781 aaagaagtcg tcggcgattc cgccgagttt ttcgcgcgcc atggcctgcc ccgtcttcgt
     3841 cgtggtggaa atcaggatcc gggcgtaggg caggcgtcgc cgcaatccgg cgaccagctc
     3901 ctggcacgaa agcacctcgc ctaccgaaac cgcgtgcaac caaatggcgc gtccgtcggt
     3961 ctcccttaaa gaggacccga accagcccag gcgctgacga agcgtgccgc ggtacttgcc
     4021 gcctctccgg ccgcgaagga gatagaaggg tacgagcacg atcgaagcca gcgccagcgc
     4081 cagattgtaa acaaacagca tggaggaaag cttgcttcta ccggcaactc agatatatag
     4141 tcccatgaag gtgctgggca aagccattac tctagccgtc cttacggcgg ggatgattcc
     4201 tctggtttcc ccggccgccg cggaaaagac gcggtttgag ccccaaaaag cagacgagta
     4261 tccggcccac cagacgagca gcaacgtgac agtagccgcg gacccttacg aaagcggtcc
     4321 caaggtgaag gatgcctttg gaaaattgaa cccggcgaag cttgggatcc tgcctgtact
     4381 gctggtgatt cagaacgatt ccgacactcc cttgcagctc gaccggatgc gcgtgcaatg
     4441 gattaccgct gatcgccaga ggttcgactc ggtctcagcc gaagacgccc agcgtagcgg
     4501 ccccatccgc cgcccggagg tgaacccgcg tccatctccc ataccaggaa tcgggcgagg
     4561 atcgaggcgg cctaaggacg aaaaagacct tgccgcgctc gaattcgtcg cgccggtggt
     4621 catgccccac agcgaggctc acggcttctt ctattttccc gccggaaaag agcgcggccg
     4681 gatcgctggc tcgaaaattt acatcaccgg aatccgggat gcgcggagcg gccg
//
LOCUS       DASREK010000275        23873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1611692, whole genome shotgun sequence.
ACCESSION   DASREK010000275 DASREK010000000
VERSION     DASREK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 23873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23873
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611692"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFA54_14840"
     CDS             complement(<1..362)
                     /locus_tag="VFA54_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS52141.1"
                     /translation="MDIIPVTAGRVAAVRTLFEEYWRSFGFTPCFQNFAAELASLPGV
                     YSPPDGRLALALASEDPAGCIALRRFDGRRCEAKRLYVRPNLRGRGIGRALLEWAIAE
                     ARKIGYEELLGDTLPLMDA"
     gene            593..1246
                     /locus_tag="VFA54_14845"
     CDS             593..1246
                     /locus_tag="VFA54_14845"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZS52142.1"
                     /translation="MSCLSRARQRIFDGIGWICLGVLLSAVSGCAGRYGRIPAARAPD
                     TAALEDRIHWQVNSAIELLTHGAPFDYIVYRLDAGTVTLMGRVTRPELKRQVEKVVRN
                     IEGVRSVADEIQVLASSPADEWLRFALYEAIYGDSALNHYAFRHRPPIYILVSRGQVT
                     LLGAVDNIWDRQIAEVEAVQLPGVVSVTNGLRVEAGYPREATPGAARAAGRRLPMDR"
     gene            complement(1640..3157)
                     /gene="xylB"
                     /locus_tag="VFA54_14850"
     CDS             complement(1640..3157)
                     /gene="xylB"
                     /locus_tag="VFA54_14850"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HZS52143.1"
                     /translation="MAYWLGIDTGTGGTRALLLDGRGRVAAGFTAPHEEVQMPHPLWA
                     EQRPQDWWQAAQAAIRGVLRQAGISGNEIAGIGLSGQMHGLVLLDAQNQVIRPAIIWC
                     DQRCQRQVDWIHGRVGRDRVVALTANPMLTGFTAPKLLWVREQDPRSFERARKVLLPK
                     DYIRFCLTGEFACDASDASGTGLFDVMNRVWSTAMIDELDLDRDLLPPTFESPALTGK
                     VAPLAAGITGLCAGTPVFAGAGDQAASAVGNGIVEPGIVSCTIGTSGVIFAHMDKIEF
                     DPEGRVHTFCHAVPGKWHVMGVTQGAGLSLQWFRNHLGTDFLNRAEDAYDLLIDEAAQ
                     APLGSAGLFWLPYLMGERTPHLDPTARGGWIGLTAQHTRKHLIRSLLEGVSYSLQDCM
                     KLIERLGIEIQFVRASGGGARSRFWRQMLADVFDKPIATLETQEGSAYGAALIAMAGA
                     GEFSGVAEACRCVVKEASRVLPQPLATAAYAERYLVFRSLYPALRDRFQAIASLA"
     gene            3413..5041
                     /locus_tag="VFA54_14855"
     CDS             3413..5041
                     /locus_tag="VFA54_14855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS52144.1"
                     /translation="MRGETQTNDEAAHLVAGYSYWTAHDFRLNPEHPPLSKLLCAAPL
                     LALRLQFPHLPGAWRNADEFALGRAFLYHNMAPAGTILLAGRSVTILLTAVLGLLLSV
                     WTRKRFGAAAALFSTFLFAADPNFLAHGRYVTSDVPVTLFFLAACLSWFGYLETSLPK
                     DLLRTGMLLGLALGTKFSSLLLYPIFLLAAMAAGWRPPAKPAGRPPIRLVACALMVVP
                     WLAVFALYMFDTRSVAADPLLSARLERAPIASSRLVRAAEHIPIPAYYWFRGMQLLYR
                     HAHGGHTSYLMGRVLRQGSWLYFPIAFLLKTPLATLALLALTMLAVAMEISGAPRAAS
                     RRNPSLPLFWLGIPPAVYFIASLGSPIDIGLRHILPIYPFLYMLIGAALFPQGEAGRP
                     GRPVRTAALALAALLAVESAGIYPHYLAFFNAAAGGPAHGARYLIDSNLDWGQDLFHL
                     KEWASAHGATPLCLSYFGEADPAYYGISYRPLGPVRDEQEEQRLDCVAAISAQHLFAA
                     TDGRFRRLQRIEPVARIGYSIFIFDLRRQRAATR"
     gene            complement(5020..6537)
                     /locus_tag="VFA54_14860"
     CDS             complement(5020..6537)
                     /locus_tag="VFA54_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52145.1"
                     /translation="MLSRGRGRAGQAGKFLAGAVLLAPLLIYWLLPTRDVYWDGVAFA
                     IQIEKAGADMGPLVHPNHLLYEVFGHWLFAASQSAGASLRALFLLQFCNGFAAFLCAV
                     LLYAILRKTGTGAFHAACWALVFAFSGTWWKFATDADAYIIAILFLLAAYALMLGGKP
                     RPAAVALAHTGGMLFHELAVFFFPAAIWKLYRQSSGSRAQKIRVAGEYFALAALLTTA
                     VYRYMFRRARLPDKRFWSWVTAYSPDAQFSFNPLRNLALTLAGTARLFFTGRADLVTT
                     RPIEIAGLILLAAIGALLLIRFRSPRAAIHRFGSDPRRGRDLIPLVIWAGSYVLFLFF
                     WMPQNTFYRLFYLPPVVLLLGAAAPQREAPRPQLALAAAFVFLWNFTFSIYPRSRIES
                     NPVLTFALKQKSAWRPGDGVAFLRFVPDLWTICYFNPQASWIGLLEAGPAELDGWRER
                     LGHGGGRLWLDETAFAALNGTAAGRSWIAQNIDFSGSIDSRLGKSRFRFYRVAAR"
     gene            7106..8668
                     /locus_tag="VFA54_14865"
     CDS             7106..8668
                     /locus_tag="VFA54_14865"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZS52146.1"
                     /translation="MVAKSADRDPALTLIDQDQERRTLRKIRGELERSWNRDSSRLLE
                     QFRVQEVKSWQQTEDLVSQQSEPLVLDEVLRLNLEEDSSLPPDRLRVLLATKSDLPLV
                     INDRVLRYVNYFLGRGQSTLRESLRRAGSYRPMISRILAEEGVPQDLIYVVQAESGFR
                     PQARSPKKATGMWQFVSGRGREYGLSQTKQLDERLDPEKATHAAARHLADLHTQFGNW
                     YLAMAAYNCGPGGVQRAVEKTGYADYWELVKRGALPRETSNYVPVILAMLLLDKNAEA
                     FHLDDIVPEDPIAYDTVQTDSRIGLSLIADAADTSVSEIKRLNPALLGESTPDGPYSL
                     RIPKDTAEDFEREIAAVPEERRSSWRRHRVLEGETLSQIASLYRVKVQDIIQVNQLGD
                     GPLRAGERLTIPVPPAPVVRRAGLKGTGGASRTGHPAQYRVKPGDSLGSIAQRYHVSV
                     AQLQAWNHLKGVRLIAGQTLNVHTVSPTPVASAGRVTRRSVTKVSQTRRAALPGGRTE
                     RTRTLVAATAAN"
     gene            9708..10571
                     /locus_tag="VFA54_14870"
     CDS             9708..10571
                     /locus_tag="VFA54_14870"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HZS52147.1"
                     /translation="MPVTAVQHFARMRGGAQAQLLLASDGHYYIVKFQNNPQHRRILA
                     NELLAEVLLRHLKLPTPPGEIVEVSEALIAATPALCMEAGGARVPCSPGLQFGSRYPG
                     DPARVPVYDYVPDALLRQVVNADCFLGMVAFDKWVSNADGRQAIFFRDQIARWRCGQA
                     PPGASEENPGSRKDERGFVAVMIDHGFAFTAHHWTFRDAPQIGIYPRPWVYERVTGYD
                     SFEPWLGWITEFSLDVLDDAFKRIPPEWYDSDMPALESLLEQLYCRRARVPDLVRSAK
                     RALRDPFPNWR"
     gene            10592..11425
                     /locus_tag="VFA54_14875"
     CDS             10592..11425
                     /locus_tag="VFA54_14875"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HZS52148.1"
                     /translation="MDHCAQPNPQASQNPPALPGGGVLYRFRLLRYAPNRLSDEFYNI
                     AALVYDEHRRLLDARFTPDFVRLRANPLADLRFLLLLKEDFENRRLLGEGFDAYVEDL
                     LENLSQGLQISEEKSFLGGAAGKEMERLVDTYLATPRRSELRAAGPQPDTRRWVLAKL
                     RDALRLHGLAERLQADVSVGAAVSPRFSFRIDYAYKPNGKTHYIQALSLRRDLNDAGR
                     LCFVFDRLRSQIPAALTAVVPDILPEDTWKLLESSDIRPWRVSQVGDLALSIRQDLQL
                     S"
     gene            complement(11615..11848)
                     /locus_tag="VFA54_14880"
     CDS             complement(11615..11848)
                     /locus_tag="VFA54_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52149.1"
                     /translation="MYGLESLSPAFIEHWAIELLRQKGRQIDNQLSTDGGLQFKIDGE
                     WRTRDELVAMARQYPEWRHLARKHEEYLREALV"
     gene            complement(12137..12550)
                     /locus_tag="VFA54_14885"
     CDS             complement(12137..12550)
                     /locus_tag="VFA54_14885"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZS52150.1"
                     /translation="MAYWLFASDPEDYHLGKLFTEKRTVWDGVHGAVARKNLSRVKKG
                     DRILGYHSAPLKSVYAELKSLTDGYEKQTKEGPAWVVDVAPVRRFKAPVPLSDLKANP
                     KLAGMTFLKIQRVSVSPVTELEYNEILRMGGVKPE"
     gene            complement(12555..13616)
                     /locus_tag="VFA54_14890"
     CDS             complement(12555..13616)
                     /locus_tag="VFA54_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZS52151.1"
                     /translation="MAASHLVTLIPGDGIGPEVAEATVRAVAAAGARIEWERVEAGAG
                     AMARTGRFLPDEVLQSIERNRVALKGPLSTPVGGGFPSANVALRKAFDLYVNFRPVRR
                     LPGVPTRFSDLPLDMIIFRENTEDLYAGLEHEVVPGVVESLKIITERASLRISRFAFD
                     FARRHGRRKISVVHKANIMKMSDGLFLRCARAAAAEFPEIQHEEIIVDNACMQLVMRP
                     ETFDVLLLENLYGDIISDLCAGLVGGLGLVPAANLGDTHAIFEAVHGSAPDIAGRNIA
                     NPTALMQSAVLMLAHLGELEAAARLQQAILQVYSEGRSLTRDVGGAAGAKEFTGAVIA
                     ALAKQDASQVALPAQPVIV"
     gene            13672..14859
                     /locus_tag="VFA54_14895"
     CDS             13672..14859
                     /locus_tag="VFA54_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZS52152.1"
                     /translation="MGEAVYFYRDLAYVFVAAVLGGLVASRLRQPLILGYVLAGVLIG
                     PFTPGPAVSDIRALELLAEIGVILLMYSIGMEFSLRELLQVKWVAILGGPAGIVLSIA
                     LGAGAGWLLGWSALQGVTVGAVISVASTMVLSRLLSDRGELQAQPGRLMIGITLIEDL
                     AVVALTILLPSLRSFHGGRLAILALEIGKSLLLLVPIVAAAFRLAPPLMARVARMQND
                     ELYVLVSLSLGFATAAATQALGLSLALGAFLAGLVISESEHSHETLRRLVPVRDAFVA
                     LFFVTIGALINPRRLVAAPGLVAVMFGLIVIGKLAIWTAVVRLFGYPLRTALLVGVGL
                     TQIGEFSYVLVRVGRDSGLVGADIYNAVLAASLLTILSNAVLMRIASAKLAQWLPAAQ
                     KRN"
     gene            complement(15035..16915)
                     /locus_tag="VFA54_14900"
     CDS             complement(15035..16915)
                     /locus_tag="VFA54_14900"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZS52153.1"
                     /translation="MSEMAARLVFVLAFAASLWEFLRTVYRRFHYKIQPTPRLPFDRV
                     PARLWRVFCEVFLQSRVIRDRPIPGLLHALVMWGFFVFAWVSVRHLSLGFLGLDAASS
                     ERTWYGAFAAVWAFAVLAGIIGLSIRRFVLRPKLLGPLSPSSGLIAFLIAALMVTYLL
                     GWGVFPLGSLAWRANWWAHTLSFFAILAEIPHSKHLHLLLGPIGIFFRSETTSSIRPL
                     RPDDEEDIGMVRFTDLGPKDLLDLHACVECGRCTQFCPANVAGQTLNPKEVILGMQRG
                     LLAGGSVIAGTVEETQAGKAWVTEEDLFQCLSCGACEQACPVGIEHVGLKILDLRRGL
                     VSEGRTTKPKVNDLFTTMERAPHNPWGVSHETRRKFIESERFPVFDGSQEWLFWMGCG
                     LNYDPHGQQVARAMRKILEAARVSWGVLAQETCCGEPGRRAGNEYLYLELSSKLIEAF
                     GRHQVKNIVTCDPHCARMFDVDYRQIPEYESLAINIAHHTEMLARLLPSLPLKPARES
                     VTFHDPCYLARGRGITREPRSILRSCGVSVAEMAHHGERTFCCGAGGAQLYIAEDIRG
                     REEQRVNHRRFAEVLATGAPAVAVACPYCPIMLKDAAAHAGREEIAVLDIAEIVAGRL
                     RA"
     gene            complement(16912..17892)
                     /locus_tag="VFA54_14905"
     CDS             complement(16912..17892)
                     /locus_tag="VFA54_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HZS52154.1"
                     /translation="MAILVVTEQYRGTWHKMSWEALAAGIEIGQALERPVWALIIGGN
                     TGALVSDLAAQKVERVIQVEHPLLAEYTPDGYVLALRQVIKQLNPYLAVMPHTYQVRD
                     FAPKLAASLGRGFISDAVGFRAEQGAPLFVRQLFQGKLNADVAFRGDPPYFASVQAGA
                     FRADRLERAGSPAPVEKPAVALEAAQIRTRPQERFQEARGTVDLASAEIVVSVGRGIQ
                     GPENLPLAEALANALGAQLGASRPVCDSGWLPLERQVGSSGQTVAPKLYIALGISGAI
                     QHLVGMKGSRTIVAINKDPSAPIFEVADYGIVGDLFQIVPALTEALQAAK"
     gene            complement(18084..18854)
                     /locus_tag="VFA54_14910"
     CDS             complement(18084..18854)
                     /locus_tag="VFA54_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZS52155.1"
                     /translation="MKIIVCLKQVPAKDSLLRIDDRQRWIQEAGLSFEINEPDAFALE
                     EGLRLKEKHGGEVIVVSAGPARAASAIREALAKGADRGIHIEDEAISRLDSFAVAGAV
                     AAALQSEPFDLILTGLQSDDLGYAQMGVVLAEMLGLPHATIIMEVEKQDSGIKVKREL
                     ESGWFQHVEMPLPALLTIQSGINKVRYATLMGIKKAKTKPLRCVTPAELGVQLEPRQE
                     IQAVYQPKKTKQTQLIEGGPKEAAAKLVEKLKFEARVL"
     gene            complement(18913..20148)
                     /gene="fabF"
                     /locus_tag="VFA54_14915"
     CDS             complement(18913..20148)
                     /gene="fabF"
                     /locus_tag="VFA54_14915"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZS52156.1"
                     /translation="MRRVVVTGVGLISAVGVGTEQTWKAITEGQSGIGRITSFDASDF
                     STQIAGEVKGFDPFQYIEKKEVKKMGRFIHFAIAASEFALKASGLNVTPEIEERVGVY
                     IGSGIGGFDVIEREHANLLSGGPKKISPFFIPATIINLASGQVSIRTGAKGPNSATAT
                     ACTTSAHAVGDSFRLIQYGEADVMICGGSEATITPMGIGGFAAMRALSTRNDDPRAAS
                     RPFDADRDGFVVGEGAGILILEELDFAKRRSAPILAELVGYGMSADAHHITSPSEDGD
                     GAYRVMRNAIRDAKIRFDQIDYINAHGTSTPVGDRIETIAIKRTFGDHARKVAVSSTK
                     SMTGHLLGGAGGLEAGIVVLALRDQVMPPTINLCNPDPECDLDYVPNFARQARIEYAL
                     TNSFGFGGTNGALVFKKFE"
     gene            complement(20282..20521)
                     /gene="acpP"
                     /locus_tag="VFA54_14920"
     CDS             complement(20282..20521)
                     /gene="acpP"
                     /locus_tag="VFA54_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZS52157.1"
                     /translation="MSVEEKVKQIIVEQLGVDEAQVDGAASFVDDLGADSLDIVELVM
                     AFEEAFEIDIPDEDAEKITTVKGAIDYIQSHAKVK"
     gene            complement(20677..21177)
                     /locus_tag="VFA54_14925"
     CDS             complement(20677..21177)
                     /locus_tag="VFA54_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZS52158.1"
                     /translation="METIESPLKKLRTALGWSQSALAQALGCSYSSIQNYEGGKRVPR
                     EIIERLKSIAAEHDLADIALELSSEAWQVRRVFQPREVRISGAAAAVSAASQKPLDGS
                     RSKDSGSDDREACHQILDRILDSGDRVAIAAVLQNLHALDLLIHSNQTKRRSGNQKTR
                     PATREK"
     gene            21373..21627
                     /locus_tag="VFA54_14930"
     CDS             21373..21627
                     /locus_tag="VFA54_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52159.1"
                     /translation="MTIKDRLDQEYRLLQVIERVRTQRGNPYIFSNAERKVVNTELAR
                     LEREMEIIWFTELVIEMSSRLTPAEIERVVGRLAGWIETC"
     gene            21687..22304
                     /locus_tag="VFA54_14935"
     CDS             21687..22304
                     /locus_tag="VFA54_14935"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZS52160.1"
                     /translation="MLRETTSAQRFPLIAIDPGHGAGWVKPNVLDEGAVAYHKGARYA
                     EQELALRYAIALDEELHKRGVNTVLTRTDSRTNSPPQRRKELCSGASLALSLHFSKSL
                     NPKDRGFELAAPPELEALSQAVRQALEMWLPCRDVSAVSSSRDCVRAVYLGCPSLEIR
                     PGVLTSCQDMELITSAYWKVLFAERIAQAVDQHFGFASCARAAAR"
     gene            complement(22389..23033)
                     /gene="lexA"
                     /locus_tag="VFA54_14940"
     CDS             complement(22389..23033)
                     /gene="lexA"
                     /locus_tag="VFA54_14940"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZS52161.1"
                     /translation="MALTRRQRQVLDYIAGFIQAHGYSPSFEEIAQAMQLASLATVHK
                     HLTTLCTKGYLKRGINQSRSIDLGPQYYSDQRRQRQERALRPAFAAELPLQGRIAAGK
                     PIEAAENPETLSFGDFLGAPNVYALQVHGESMIDDHIMDGDYVLVQRTEEVRDGDIVV
                     ALVEGTETTLKRFYREPNGMARLQPANSAMQPIIVPLEGVRIQGRVLAVHRRYR"
     gene            23629..>23873
                     /locus_tag="VFA54_14945"
     CDS             23629..>23873
                     /locus_tag="VFA54_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52162.1"
                     /translation="MGTIESRWRQSLTRRRALQGLAAFLASSPLLDSQQDPFRDHSRV
                     PALNELLTSFDFEPVFFAKEPRAIYDYTSYGAESEFT"
BASE COUNT         4664 a         7353 c         7242 g         4614 t
ORIGIN      
        1 gcatccatca gcggcagcgt gtccccaagc agctcctcat aaccgatctt ccgcgcttct
       61 gcgatcgccc attcgagcag cgcgcgcccg atgcctcgcc ctcgaagatt cggccgcaca
      121 taaaggcgct tggcttcgca ccgccggccg tcgaaacggc gtagggcgat gcacccggcg
      181 ggatcttcac ttgccagagc cagggcaagc ctcccatccg gcgggctgta gacgccgggg
      241 aggctggcca gctctgcggc gaaattctga aagcacggcg tgaagccgaa cgacctccag
      301 tattcctcga acagggtgcg taccgcagcc actctgcccg ccgtgaccgg gatgatgtcc
      361 acagcaccag tgtacgcccg gccacccgct ttgtacggcc tgcctggcga aatgctcgca
      421 ctgcttggac tcagcagagc atcttgtgaa tgaagaaagc cgggtcgcac gcctcgaggc
      481 ggcgcaaagc tgcgaacggg cctcgttgtg actgatcgcg catgccgtga atggaacgaa
      541 gcgcacagac aacccccttg gctggacgcg atgatagccg gggagattgc ttatgagctg
      601 cctttccagg gcgcgacaga gaatattcga cggaatcgga tggatttgcc tcggtgtcct
      661 gctttcggcg gtgagcggct gcgccggccg atacggccga atcccggcgg cccgggcgcc
      721 ggacacagcg gctctcgaag accgcattca ctggcaagta aacagcgcga tcgaacttct
      781 tactcacggc gccccgtttg actatattgt ctaccgcctg gatgccggca cggtgacgct
      841 gatgggcagg gtgacccgcc ccgagctgaa acgtcaagtc gagaaggtcg tgagaaacat
      901 cgaaggcgtt cggtcggtag ccgatgaaat tcaggttctc gcttcttcgc cggccgatga
      961 atggctgcgc ttcgcgctct atgaggcaat ctacggagac tcggcgctga accattatgc
     1021 cttccgccat cgcccgccta tttacatcct tgtaagccgc gggcaggtga ccttgctggg
     1081 agcagtcgat aatatttggg accgtcaaat cgcggaggtc gaggcggttc agttgcccgg
     1141 cgttgtgtcg gtgactaacg ggttgcgtgt ggaagcggga tatccgcgcg aagcgacccc
     1201 tggggccgcg cgagcggcgg ggcgtcggct gccgatggac cggtaagttc aaaaccctct
     1261 gggtttgttt cacagacggg gcccagccga cgccgctatt cagaaagcgc gctgatcggg
     1321 atggaaaagc cgggaggaga tcgggggcgt cgaccctgcc gccgggatac aaaacacagc
     1381 cgctgccgga agcctcgaag atctgaacct ctttcgtctc cggtagaaaa tccagactac
     1441 tcgggccccg ttaatactgc cggatttttc gcggcagtgg ggggacggaa ttggaccgct
     1501 gagaaggttt caacccgctt ttcgaagggc gcgcagtcag ccggtcgctg cccgaagctg
     1561 gcgcccgagt cgtcagtcct ctctcgtcca gttcctactg atccacgctg aggagggtct
     1621 tgctgcttct atttggattt tacgccagtg aggcgattgc ctgaaaccga tcgcgcagtg
     1681 cgggatacag gctgcggaaa accaagtaac gctccgcata ggctgcggtc gccagcggct
     1741 gagggagcac ccggctagcc tctttgacga cacaacggca agcctctgcc acaccggaaa
     1801 actcccctgc gcctgccatc gcgatcaggg cggccccgta agccgagccc tcctgcgttt
     1861 cgagcgtggc gatgggtttg tcgaaaacat ccgccagcat ctgccgccag aaccggcttc
     1921 gcgctccgcc ccccgaggcg cgcacaaatt ggatttcaat tcccaatctt tcaatcagtt
     1981 tcatacaatc ttgcaagcta tacgagaccc cctccagcaa ggatcggatc aggtgtttgc
     2041 gagtatgttg ggcggtcaag ccgatccatc ccccgcgcgc agtagggtcg aggtgggggg
     2101 tgcgctcacc catgagatac ggcagccaga aaaggccggc ggatccaagc ggggcttgtg
     2161 cggcctcatc gatcagcaaa tcgtacgcat cctccgcccg gttcaggaag tcagtgccga
     2221 ggtgatttcg gaaccattgc aggctcagtc cagccccctg cgtaaccccc atcacgtgcc
     2281 acttgccggg aacagcgtgg cagaatgtat gaacacggcc ctcggggtcg aattcgattt
     2341 tatccatgtg tgcgaatatc acgccggatg tcccgatcgt gcaagagaca atgcccggtt
     2401 cgacgattcc gttgcccacg gcgctcgccg cttgatcccc cgccccggcg aacacgggcg
     2461 tgccggcgca gaggccggtg attccggccg cgagcggcgc aacttttccg gtgagtgcgg
     2521 gcgactcgaa ggttggcggc agaaggtcgc gatcgagatc gagttcatcg atcatggctg
     2581 tggaccaaac ccggttcatc acatcgaaca acccagtgcc ggaggcatcg ctcgcgtcgc
     2641 aggcaaactc gccggtgagg cagaaacgga tgtagtcctt aggcagcagt accttccgcg
     2701 ctcgctcgaa cgaacgcgga tcttgctccc gcacccacag cagcttcgga gctgtgaagc
     2761 cggtcagcat cgggttggcc gtcaaggcga caacccggtc tcggcccacg cggccgtgaa
     2821 tccaatcgac ctgccgctgg cagcgctggt cgcaccagat aatcgccggg cggatgactt
     2881 ggttttgggc atccagcaga accaaaccgt gcatttgacc ggagaggccg attcccgcaa
     2941 tctcattgcc ggagataccc gcttggcgca agactcctcg aatggccgcc tgcgctgcct
     3001 gccaccagtc ttgcggacgc tgctcggccc agagcgggtg cggcatttgt acctcttcgt
     3061 gcggcgcggt gaaacctgcg gccacgcgtc cacgcccatc cagcaggaga gcgcgggttc
     3121 cgcccgttcc ggtgtcaatg cccagccaat atgccatcgc ctttgagtcg cctctctaca
     3181 cgaaaaaccg gctgcctcga aaccgcccag tataccgctc cgcgtccgag ctcttccgca
     3241 agccgcagga cggggcagtc gcgcctagcc ggtggccccg cggtggggcc gccacggaga
     3301 tcccgtttcc cgcgatccca tgttgaagag taatccgaat tttgtggatc ggtgctactg
     3361 gccctgtgtc gggctcctac tcggcgcaat gatggcgctt caggttttat ccgtgcgcgg
     3421 ggaaactcag acgaatgatg aagccgccca tctcgtggcg ggctacagct attggacggc
     3481 tcacgatttt cggctcaacc ctgagcatcc tccactttcc aagctgcttt gcgccgcccc
     3541 gttgcttgct ctcaggctgc aattccctca tttacccggc gcctggcgca atgcagacga
     3601 gtttgcattg ggacgcgcgt tcctgtatca caacatggcg cccgctggaa ccatactcct
     3661 ggccggaagg tctgtgacca tcttgctaac agccgtcctc ggtcttctct tatccgtttg
     3721 gacccgaaaa cgattcggag cggcggctgc tctattcagc acgtttttat tcgcggcgga
     3781 tccgaatttt ttggcccacg gccgctatgt gacctccgac gttccggtaa ctctgttttt
     3841 cttggccgcc tgcctgagtt ggttcggcta tcttgagacc tcgctgccga aagacctgct
     3901 ccgaaccggg atgctgttgg gactcgctct ggggaccaag ttcagttccc tgctcctata
     3961 tcccattttc ctattggcgg ccatggctgc cggctggcgc ccgccggcta agccagccgg
     4021 caggccaccc atccggctag tggcctgcgc tctcatggtt gtgccgtggc tggccgtgtt
     4081 cgctctatat atgttcgaca cccgctccgt ggcggccgac ccattgctgt cggctcggct
     4141 ggagcgcgcc ccgatcgctt ccagccgcct tgtgcgcgca gccgagcata ttccgatccc
     4201 ggcgtattac tggttccggg gcatgcagtt gctttaccgg cacgcccatg gaggccacac
     4261 cagctatctc atgggaaggg tcctccgcca gggatcctgg ctgtacttcc caatcgcctt
     4321 cctcttgaag acgcccctgg cgaccctggc tctgctcgcg ttgaccatgc ttgccgttgc
     4381 catggagatc tcgggagcgc cccgtgcggc aagccggcga aacccgtctt tgcccctgtt
     4441 ttggctgggc attcctcccg cggtctactt catcgccagc ctgggcagtc ccatagatat
     4501 cggcttgcga catatactcc cgatctatcc gtttttgtac atgctgatcg gggcggcttt
     4561 gttcccgcaa ggcgaagctg gcaggccggg acggcccgtg cggacggcgg ccctagccct
     4621 ggctgcgctg ctggccgtgg aatccgccgg aatttatccg cattatcttg cattcttcaa
     4681 tgccgcagcg ggcggaccgg ctcatggtgc gcgttatctg atcgactcca atctcgactg
     4741 gggccaggac ttgttccact tgaaggaatg ggcgagcgct catggcgcca cacctttatg
     4801 tctcagctat ttcggcgagg ctgatccggc ttattacggc atctcctacc gcccgcttgg
     4861 tccggttcgc gatgagcagg aagagcagcg gctcgattgc gtggccgcaa ttagcgcgca
     4921 gcacctgttc gccgctacgg atggccggtt ccggcgattg cagaggatcg aacccgtcgc
     4981 gcgcataggc tactccattt ttatctttga tttgcgccgt cagcgcgccg ccacccggta
     5041 aaagcgaaag cggctcttcc cgagccggga atcgatcgat ccggagaaat cgatattttg
     5101 cgcaatccag ctcctacctg ctgcggtccc gttgagcgct gcaaatgccg tttcgtccaa
     5161 ccagagccgg cccccgccgt gtcccaggcg ctcccgccag ccgtccaatt ccgcggggcc
     5221 ggcttccagt aagccaatcc aggacgcttg gggattgaag tagcagatcg tccagaggtc
     5281 gggcacgaac cgtagaaaag cgacaccgtc gccgggccgc cacgcggatt tctgtttcag
     5341 ggcaaatgtg agcaccgggt tgctctcgat ccgcgagcgc ggataaatgg aaaacgtgaa
     5401 gttccacagg aacacaaatg cagccgccag ggcgagctgc ggtctcggag cctcgcgctg
     5461 gggagcggct gccccgagca ggagcacaac cggcggcaag taaaagagcc ggtaaaacgt
     5521 gttctgaggc atccagaaga aaagaaacag aacgtaggag ccagcccata tcaccagggg
     5581 gatcagatcc ctcccgcgtc tgggatctga gccgaaccgg tggatcgctg cccttgggga
     5641 gcgaaatcga atcagcagga gcgcgccaat cgccgcgagc aggatgagac cagctatttc
     5701 gatcggccga gtcgtgacga ggtcagctct gcccgtgaaa aacaggcgcg ctgtgcctgc
     5761 gagcgtcagg gccaagttgc ggagcgggtt gaaggagaac tgagcgtccg gcgaataggc
     5821 agttacccag ctccaaaacc tcttgtccgg caggcgcgct ctccggaaca tgtaccggta
     5881 aacggccgtc gtcaagagag cggccagggc gaaatactcg ccggctacgc gtattttttg
     5941 agcccgcgac ccgctgcttt ggcgatagag cttccaaatc gcggccggga agaaaaatac
     6001 ggcaagttcg tgaaagagca ttcctccggt atgcgcgaga gccacggctg ccggccgcgg
     6061 cttgccgccg agcatcaagg cataagccgc cagcagaaac aggatggcga tgatgtacgc
     6121 gtccgcatcc gttgcgaatt tccaccacgt gccggaaaac gcaaatacca gagcccagca
     6181 cgcggcatga aacgcgccgg tcccggtttt gcgcagaatc gcatagagca acacggcaca
     6241 cagaaaggcg gcgaagccat tgcagaattg gagcaggaac aacgcccgga gggacgcgcc
     6301 ggccgattgg ctggcggcga acagccaatg tccgaatact tcgtagagca ggtgattcgg
     6361 atgaacgaga ggccccatat ctgcgcccgc cttctcgatc tggatggcaa aagccacacc
     6421 gtcccaatac acatcgcgag taggtagcag ccagtagatt agaagcggcg ccagcagcac
     6481 ggcgccagcc aggaattttc cggcctgccc ggcgcgaccc cgccccctgc ttagcataca
     6541 gagaagccgg cagggagccc catcggggct ggcccggaat tcggggaggg tacgccacac
     6601 atagacatac gctggcggca aagagcggac ggccagttcc ggcgccaatg gaaagctaag
     6661 agagtcatct tacacggtct agggttaagc ttacatagcc tgagaaaaaa gcgttctctt
     6721 ccaagcgacc cttcgagcgt gaaaagccta acattttcat gttgttctgc tactttggtt
     6781 taccaattgc tccagtctcg caccaatgac ggccgcactt ttttctggac agcttcgccc
     6841 ggatctgata ggatgcccgt cttgcctcgc ggttgatgaa aacccgtatt ctcttcgcgt
     6901 tggcgagaaa ttgtctagca ctgggatggc gaaaatccgt ttgatttggg ccgcggcttg
     6961 tctgtccgcg acggtcctgc tgagttcttg caacagtata gtggtgcgcc aagccgagtt
     7021 aatgtcagcc ctgccacgca cctggacgcc agccccccgg ggaataaacc ccgcgccttt
     7081 gctcgacccc ccgcctgtgc tcccgatggt ggcgaaatct gcggatcgcg atccggcgct
     7141 gaccctgatc gatcaggatc aggagcgcag gacgttgagg aagatacgcg gcgaactcga
     7201 gcgcagttgg aaccgcgatt cgagccggct gctcgagcag ttccgcgtgc aggaggtgaa
     7261 atcctggcaa cagacggaag atttggtttc gcagcaatcc gagccattgg ttctggatga
     7321 ggtgctgcgc ctcaatctgg aagaggattc cagtctgccg cccgaccgct tgcgcgtgct
     7381 gctggccacg aaatcggatc tgcctctggt aatcaacgat cgtgttttgc gctacgtgaa
     7441 ctacttcctg ggcaggggcc agagtacgct gcgggagagc ctgcgccggg ctggaagtta
     7501 ccggccaatg atctcgcgca ttctggccga ggagggcgtg ccgcaagatc tgatttacgt
     7561 ggttcaagca gagtcggggt tccgtcctca agcgcgctcc cccaagaagg ccaccggcat
     7621 gtggcagttc gtgtcgggcc gggggcggga gtatggactc agtcaaacca aacagctcga
     7681 cgaaaggcta gatccggaaa aagccaccca tgccgccgcc cgccatttgg cggatctgca
     7741 cacccagttt ggaaactggt acctggcgat ggccgcttac aactgcggcc ccggcggagt
     7801 gcagcgggcc gtcgagaaaa ccggctatgc ggattattgg gaactggtaa agcgcggcgc
     7861 gctcccgcgg gagacgtcga attacgtacc ggtcatcctg gccatgcttc tgttggacaa
     7921 gaacgcggaa gcgtttcacc tggacgacat cgtacccgaa gatcccatcg cttacgacac
     7981 ggtgcagacg gactcgcgca ttggtttgag cctcatcgcg gatgcggcgg acacctcggt
     8041 ctccgagatc aagaggctca atccggcctt gttaggagaa tcgacgccgg acgggcctta
     8101 ctcgcttcgt atcccgaagg acaccgctga ggactttgaa cgcgaaatcg cggcggtgcc
     8161 tgaggagcgc cggagtagct ggcggcggca ccgcgtcctg gaaggcgaaa ccttatccca
     8221 gatcgccagc ctgtaccgcg taaaagtcca ggacatcatc caggtgaatc agttaggaga
     8281 tggtccgttg cgggccggag aacgcttgac gattcccgtg ccgccggctc cggtggtccg
     8341 gcgagccggc ttgaaaggga cgggcggggc ctccagaacc ggccatccgg ctcagtatcg
     8401 agtgaaaccc ggcgattcct tgggttcgat cgcacaacgc tatcacgtaa gcgtcgccca
     8461 gttgcaggct tggaatcatc tgaaaggcgt ccggctgatc gccggacaaa ctttaaacgt
     8521 ccacacggtc tccccaaccc ctgtagcctc ggccggacga gtgacccgga ggagtgtaac
     8581 taaagtatcg cagacacgcc gagccgcctt gccgggcggt cgaacggaac gcactcgaac
     8641 tctcgttgcg gcgaccgccg cgaattaggg aaaagaacgg ataaattttt cagttcttgc
     8701 ttgccaggat ccgaaaaagc gctattctat agttcccacg cttggcatca ggcagagatc
     8761 gatgccacgg tgtgtacggc ggttaaattc attcaacggt agccggcgct ggaaagcgcc
     8821 cggtcaggag gcgcgtatat ggcttttcgt gacccggaaa actttggtct gaacgacgaa
     8881 gagattgaag agtgcccgga gttggacgat gaagccggtt ttgagcacga ggcggaagaa
     8941 gagtctgaag tagaaccgga catggaagaa ccgggagccg ccgccccgcc ggcggagccg
     9001 cccgctccgg ccgttccccc gaaaggcgaa aaggccggcg agcccgcgaa aaagccggcc
     9061 agaaaagcca agacagccgc aaggaaaaag cccgccctca aggccgccaa gaaagctgct
     9121 ccgaagaaaa ctgcacggaa aactgcgtcc aggaaagcct ctcccaggaa agccgcaaag
     9181 agaaccgcga gtcgaagggc agcgcctaaa aaggctgtgg cgcgtggtcg aaaaagttcc
     9241 gggaagggcg cttccgcgaa gaaaaagggc ggcaggcgtt aacacctgcc ggcgaagcac
     9301 tgcgggcgcc gtatggagtc tcctccggcg cccgtgcctg cgggggatca agcggttctc
     9361 ttcttttcaa ccgtttcgcg gcatttagcg tataactgca agaggctttc ggcgtactct
     9421 tcattgctcc gagccgctgg tttgccggaa aaaccggctc ccgtttgcat ccggccatag
     9481 ccgctggtta cggcgatgaa cttcatcagg tcaagagcga tgtcttcggt gctccggggg
     9541 ccgaacggtg gtaaggaagg gaaactggac tccgccatag ttatctccat caaaacggat
     9601 tttcaacggg cgcgcagccg cacgcgcatg agcgcggcct gctggcgtct gtttcgctgc
     9661 cattgtacga tggactggac agtgaaagcc ccgcggcaga ttcgaacgtg ccggtcacgg
     9721 ctgttcagca cttcgcgcgc atgcgcggtg gagcgcaggc ccagcttctg ctggcctccg
     9781 acggccacta ttacatcgtt aagtttcaaa ataaccccca gcaccggcgc atccttgcca
     9841 acgaactgct ggcggaagtg ctcctgcgtc acttgaagct gcccaccccg cccggcgaaa
     9901 tcgtcgaggt gagcgaggct ttgattgccg ctactccggc gctctgcatg gaggcaggcg
     9961 gcgcccgcgt gccctgctcg ccgggattgc aattcggctc gcgctatccg ggggatcctg
    10021 cccgcgtccc ggtatatgat tacgtgccgg atgctctgct gcggcaggtg gtgaatgccg
    10081 attgtttcct tgggatggta gcattcgata aatgggtttc caacgccgac gggcgccagg
    10141 cgattttctt ccgggaccag attgcccgct ggcgctgcgg ccaggcaccc ccgggcgcct
    10201 ccgaagagaa ccccggctcg cgaaaagacg agcgcgggtt tgtggccgtg atgatcgatc
    10261 atggcttcgc ctttaccgcg caccactgga cctttcgcga cgctccgcaa atcggcattt
    10321 acccgcggcc ctgggtttac gagcgggtga ccggttacga ctccttcgag ccttggctcg
    10381 gctggattac cgagttcagc ctggacgttc tggatgacgc tttcaagcgc attccacccg
    10441 aatggtatga ctcagacatg cccgctttgg aaagccttct cgaacaactc tattgccggc
    10501 gcgccagggt tcccgatctg gtgaggtccg ccaagagagc gcttcgagac ccgttcccga
    10561 attggcggtg accgcattcc agaggaagag aatggaccac tgcgcacagc ccaatcctca
    10621 agcttcccaa aacccgccgg cgcttcccgg cggcggggtg ctgtacaggt tccgcctcct
    10681 gcgctacgcc ccgaaccgct tgtcggatga gttctacaat atcgccgcgc ttgtctacga
    10741 cgaacatcgc cgcttgctcg acgcccggtt cacgcccgat ttcgtccggc tgcgcgcgaa
    10801 tcctctcgcc gaccttcgat tcctgctgtt gctgaaggaa gacttcgaaa accgccggct
    10861 tctgggcgaa ggcttcgatg cctacgttga ggatctactc gagaacctat ctcagggatt
    10921 gcaaatttcc gaggagaaat cgtttctcgg cggcgcagct ggcaaggaaa tggaacgcct
    10981 ggtcgacacg tatctggcga ctccccggcg gtccgagctg cgcgcagcag gcccccagcc
    11041 cgatacaagg cgctgggtgc tggcaaagct gcgcgacgct ctgcggctcc acggcctagc
    11101 ggagcggctc caggcggatg tttctgtagg ggcagccgtc agcccccggt tttcctttcg
    11161 catcgattac gcttataagc cgaatgggaa aacgcactac atccaggcgc tctcgcttcg
    11221 ccgcgatctc aacgacgccg gccgcctctg tttcgtcttt gatcgcctca ggtcgcagat
    11281 tcccgccgcg cttacggccg tggtgcccga cattttgccg gaggacacct ggaagctgct
    11341 tgaatccagc gacattcgtc cgtggcgtgt atcccaggtg ggcgacctgg cgctttcgat
    11401 ccgccaggat ctgcaactct cataaacgtc agccgccgtt tgggggtcaa acaaaggaaa
    11461 acagccatcc tttgtccgga agcgggcaac ctcccttcgg gaggggaaaa gagcgcctga
    11521 tccaagaaaa ccgcaggggc cgtcagcgga agatcattcg aatcccgtcg aacgcatacc
    11581 ccgccgtgcg tcgcttggct gcaaagcagt aggtttagac caacgcctct ctgaggtatt
    11641 cctcgtgttt gcgagccaga tgccgccact ccgggtattg ccgagccata gccacgagct
    11701 cgtcgcgcgt gcgccactcg ccgtcgattt tgaactgtaa tccgccgtca gtcgagagct
    11761 ggttgtcgat ctgccggccc ttctgccgca gcaattcgat ggcccagtgc tcaatgaatg
    11821 ctgggctaag actttccaac ccgtacattg aacacctcca cctctagggg tgatccagca
    11881 acaccgttcg cgctcgaaat tcggtcccca aaagagcgcc gcagtttttt tctgatggat
    11941 ttgacggcag agcgcgttcc ctggttgctg ttgcgccgcg cgctcagtgt tatgagcgca
    12001 acaacgcact cttcaaacct gttattttgg cgccggccgc aggcctggcc ggctggttcg
    12061 aggcgtcaaa tcttgcgcag gggaacggaa tcgcagctca acgcgcgccg gaaaacacca
    12121 gtcccgaaag ccggtttcac tcgggcttga cgccgcccat gcgcaggatt tcgttgtatt
    12181 caagctccgt gaccggtgaa accgatacgc gctgaatctt caggaaagtc atgcccgcta
    12241 gcttgggatt cgctttgaga tcggaaagcg gaaccggcgc cttgaagcgg cggaccgggg
    12301 cgacgtccac gacccaggcc ggcccttcct tggtttgctt ttcgtagccg tcagtcagcg
    12361 atttcagttc cgcataaacg gacttcagcg gcgcgctatg gtagcccagg atgcggtcac
    12421 cttttttgac gcgggaaaga ttcttacgcg cgacggcgcc gtgcacgccg tcccagacgg
    12481 tccgtttctc cgtaaagagc tttcccaggt gatagtcttc cgggtccgat gcgaacagcc
    12541 agtatgccat gcagctaaac tatcacgggc tgcgcgggca atgccacttg tgacgcgtcc
    12601 tgcttcgcca gggcggcgat gacggcgcca gtgaattctt tcgcgccagc ggcgccgcca
    12661 acgtcgcggg tcagcgaacg gccttcggaa tagacttgca aaatggcttg ctgcagccga
    12721 gcggcggcct ctaattctcc caggtgagcc agcatgagaa cggccgactg catcaatgcg
    12781 gtgggattgg cgatattgcg cccggctata tccggagcgc tgccatggac ggcttcaaaa
    12841 atagcatggg tatcgccgag gttcgcggcg ggaacgagac ccagcccgcc gaccaggccc
    12901 gcacacagat cggagatgat gtcgccgtaa agattctcga gcagcagcac gtcgaaggtt
    12961 tccggccgca ttacaagctg catgcaagcg ttgtcaacaa tgatctcttc atgctggatt
    13021 tcaggaaact cagcggccgc tgcgcgggcg cagcgcagga aaaggccgtc gctcatcttc
    13081 atgatgttgg ccttgtggac gaccgatatc ttcctgcgcc catgccgccg ggcaaagtcg
    13141 aaggcgaacc tggaaatccg taaggaagcc cgctcggtga tgatcttcag gctttcgaca
    13201 acgccaggca cgacttcgtg ctcgaggcca gcatacagat cttctgtgtt ttcgcgaaaa
    13261 atgatcatat cgagcggaag atcggagaag cgggtaggaa cgcccggaag ccgccggacg
    13321 ggacggaagt tcacataaag atcgaaggct tttcggagcg ctacgttcgc gctaggaaaa
    13381 ccgccgccca ccggcgtgct caacggacct ttcagagcga cgcggttccg ctcgatggat
    13441 tgcagcacct cgtcgggcaa aaatcgtccg gtccgcgcca tcgctccggc tcccgcttcg
    13501 acgcgttccc actctatccg ggcgcccgcc gctgccaccg cgcggactgt ggcttcggcc
    13561 acctccggcc cgatgccgtc ccccggtatt aacgtgacca gatgcgaagc cgccacgatt
    13621 cgattgtaac agcggcctcg ggcccgatcg acccgatgcg agaatgacgg aatgggggag
    13681 gctgtctatt tttatcgcga tctggcgtat gtctttgtcg cggccgtcct cggaggtctt
    13741 gtggcctcgc gactgcgcca gcccctcatc ctgggttacg tccttgcggg cgttttgatc
    13801 gggccgttca cgccggggcc ggccgtttcc gatattcgcg ctctcgagct gctcgccgaa
    13861 atcggagtga tccttctgat gtactccatc ggtatggagt tttccctgcg cgaacttctc
    13921 caagtgaagt gggtggcaat tctcggaggc ccggccggca tcgtgctttc catcgcgctc
    13981 ggcgccggcg cgggatggct cctcggctgg agtgcgctgc aaggcgtgac cgtcggggca
    14041 gtgatttccg tagccagcac catggtcctt tcgcggctcc tctccgatcg cggtgaactg
    14101 caagcgcagc ccgggcggct gatgatcggc attacgttga tcgaggattt ggcggtcgtg
    14161 gcgctcacga ttcttctgcc cagcctgcgc tccttccacg gcggccggct ggcgatcctt
    14221 gcgctcgaaa tcggcaagtc tctgctgctg ctcgttccca tcgtcgctgc cgccttcagg
    14281 ctggcgccgc cgttgatggc cagagtggcc cggatgcaaa atgacgagct ttatgtgctg
    14341 gtatcactct cactgggctt cgccaccgcc gcagcgactc aggcgctcgg cctttcgctc
    14401 gctttggggg ctttcctcgc gggtctggtg atcagcgaat ccgagcactc gcatgagacg
    14461 ctgcgccggc tggtgccggt gcgggacgcc tttgtcgcgc ttttcttcgt caccataggc
    14521 gcgcttatca atccccggag actggtagcg gcgccggggc tcgtcgccgt gatgttcggc
    14581 ttgatcgtga tcggaaagct cgccatctgg accgccgtgg tccggctttt cggctatcct
    14641 ctgcgcacgg cgctgctggt cggagtaggt ttgacgcaga tcggcgaatt ttcatacgtg
    14701 ctggtgcgcg taggacgaga ttccgggctc gtgggagccg atatctacaa cgccgttctg
    14761 gccgcttctc tactgacaat tctgtcaaac gccgtgctga tgcgaatcgc ttctgcaaaa
    14821 ctggcccagt ggctcccggc agcccaaaaa cgcaactgac cccgctcaag ccggatttcg
    14881 caattctcaa agcggtacac gcgtcggctc ggcggcgcag gagccgtatg gaccgcccct
    14941 cccggaccac acgccggctt tatagagatc cggtccgagg ctgtatacgg caaacgaaag
    15001 cgtacccgtc ttcccaggga acgctggcct cggctcacgc cctgagccgc ccggcgacga
    15061 tttccgcgat gtccagaacg gcaatctcct cccggccggc atgcgcggcg gcatccttca
    15121 acataatcgg gcaataggga caggccacgg cgacggcggg cgcgccggtg gcgagcactt
    15181 cagcgaaccg ccggtggttc acacgctgct cctcgcggcc gcgaatgtct tcggcgatgt
    15241 aaagctgggc gccgccggct ccgcagcaga acgtgcgctc cccgtggtgg gccatttcgg
    15301 cgacggaaac gccgcacgag cggagaatcg agcgcggttc ccgcgtgatg ccccggccgc
    15361 gcgccagata gcaggggtca tggaatgtga ccgactctcg cgcgggtttg agcggcaagg
    15421 aaggaagcag ccgggcgagc atctccgtgt ggtgtgcgat gttgatggca aggctctcgt
    15481 attcggggat ctggcgataa tcgacgtcga acatgcgcgc gcaatgggga tcacaagtga
    15541 cgatattttt cacctgatgg cgcccaaacg cttcgatgag cttggaggaa agctcaaggt
    15601 aaagatattc gttgcctgcc ctgcgtcccg gctcgccgca gcaagtttcc tgggctagca
    15661 cgccccagga aacgcgagcc gcttcgagga tcttgcgcat agcccgggcg acctgctggc
    15721 cgtgcgggtc atagttcagg ccgcacccca tccagaaaag ccattcctgg ctgccatcga
    15781 aaactggaaa tctctcggac tcgatgaatt tccggcgggt ctcgtgggaa actccccagg
    15841 gattgtgcgg ggcgcgctcc atcgtggtga acaggtcgtt gactttcggc ttcgtcgtgc
    15901 ggccctcgct gaccaggccg cgccgcaggt cgagaatttt caggcccacg tgctcgatgc
    15961 ccaccgggca ggcctgctcg caagcgccgc acgaaagaca ctggaacaga tcttcttcgg
    16021 tgacccacgc tttgccggct tgcgtttctt ccaccgtgcc ggcaatgacc gacccgcccg
    16081 ccagcagccc ccgctgcatt cctaatatca cttccttagg attcaaggtc tggcccgcaa
    16141 cgttggcagg acaaaactgg gtgcagcggc cgcactcgac gcaggcgtgc aggtccagca
    16201 aatctttcgg gcccaaatcg gtgaagcgga ccatcccaat gtcctcctca tcgtccggcc
    16261 ggagagggcg gatgctgcta gttgtctcgg agcgaaaaaa tatcccgatg gggccgagca
    16321 gcagatgaag gtgcttcgag tggggaatct cagcgagaat cgcaaagaag gagagcgtgt
    16381 gggcccacca gttggcccgc caagccaggc tgcccagcgg gaacacgccc cagccgagca
    16441 gataggtgac catcagagcg gcgatgagaa acgcgatcag gcccgagctc ggtgaaagcg
    16501 gtccgagcag cttcggccgc agaacaaatc gccggatcga aagcccgata attcccgcca
    16561 gcactgcgaa agcccagacg gcggcgaagg ccccatacca ggtgcgctcg gacgaagcgg
    16621 catcgaggcc gaggaagccg agggaaagat ggcggacgct cacccaggca aaaacaaaaa
    16681 agccccacat gacgagcgcg tgcagcaagc cgggaatggg ccggtcgcga atcacccgcg
    16741 attgcaaaaa aacttcgcag aaaacccgcc agagccgggc cggaacgcgg tcgaaaggca
    16801 accggggcgt aggctgaatt ttgtaatgga accgccgata gacggtcctc aaaaattccc
    16861 aaaggctcgc ggcaaaggcc agcacgaaga cgagccgcgc ggccatttcg ctcatttggc
    16921 agcctgaagc gcttcggtca gagccggtac gatttggaag agatcgccga caataccgta
    16981 atcggccact tcgaaaatgg gggcgctggg atctttattg atagcgacga tcgtccgcga
    17041 acctttcatc cccacaaggt gctggatggc gcctgagatg ccgagggcga tgtacagctt
    17101 gggggctact gtctggcccg agctgccgac ttggcgttcc agcggcagcc atccactgtc
    17161 acaaaccggg cgcgaggcgc cgagctgggc gcccagcgcg ttggcgagag cctccgccaa
    17221 gggtagattt tcaggaccct ggatgccgcg tcccactgaa acaacgatct cggccgaagc
    17281 aagatcgact gttccccggg cctcctggaa gcgctcctgc ggcctagttc ggatctgggc
    17341 ggcttccagc gcaaccgctg gcttttcgac gggagcaggg gacccggccc gttcgaggcg
    17401 atcggcccgg aaagcgcccg cctgcaccga ggcgaaatac ggcggatcgc cgcggaatgc
    17461 cacgtccgcg ttgagcttgc cttgaaagag ctggcgcaca aatagcggcg ccccttgctc
    17521 ggcgcgaaag ccgacggcgt cggagatgaa tccgcggccg agagatgcgg caagcttggg
    17581 cgcgaaatcc cggacctggt aagtatgcgg cattaccgca agatatgggt tgagttgctt
    17641 gatcacctgc cgcagcgcca gcacatagcc gtcgggagtg tattccgcca gaaggggatg
    17701 ctccacctga atcacccgct ccactttttg tgccgcgaga tcggagacca gcgcgccggt
    17761 attgccgccg atgatcaaag cccatacagg cctctccagg gcctggccga tctcgattcc
    17821 tgcagccaga gcctcccagg acatcttgtg ccaggttcct cgatactgct cagtgactac
    17881 cagaatagcc atgaaaatgc ctcggccgtc gacgcaaagt ccccgccgat taggatccaa
    17941 tgcatcgttt caaccatagg ttttccggga gcgcaaatcg gccgcaagcc ccggcggccg
    18001 cgcgtccgat tcttccggca gccatgaaag cggacggcgc agctttcctg gatacgttct
    18061 tgcatgaact acattcgatg cgctcataac acgcgcgctt cgaatttgag cttctccacc
    18121 agcttggccg ccgcttcctt cgggccgcct tcgatcaact gggtctgctt cgtctttttt
    18181 ggctggtaaa cggcctggat ttcctgccgc ggctcgagct gaacgcccaa ctcggcagga
    18241 gtgacgcagc ggagcggctt tgtcttggcc tttttgatcc ccatcagcgt ggcatatcgc
    18301 accttgttga tgccggactg aatcgtcagc agcgccggca ggggcatttc cacatgttgg
    18361 aaccagccgg actcgagctc ccgttttacc ttgatgccgg aatcctgttt ttcgacctcc
    18421 atgatgatcg tagcgtgagg cagcccgagc atttcggcga ggaccacgcc catctgcgca
    18481 tagccgagat cgtccgattg caggccggtg agaatcaaat cgaaaggctc ggattgcagc
    18541 gcggcagcca cagcgccggc tacagcgaag ctgtccaggc ggctgatagc ctcgtcctcg
    18601 atgtggatgc cgcgatcggc gcccttggcc agggcttcgc gaatggcgga agcagccctt
    18661 gcggggccgg cggaaacgac aatcacctcc ccgccgtgtt tctccttcag gcgcaatccc
    18721 tcctcgaggg caaaagcatc cggctcgttg atttcgaagc tcaggccggc ctcttgaatc
    18781 cagcgctgcc ggtcgtcgat gcgcagcagc gagtctttcg ccgggacctg cttgaggcag
    18841 acgataattt tcattttagg gccggcggcc tgcggcgccg agcaagcggc tcagcagcca
    18901 caggtccgga gcttactcga attttttgaa caccagcgcg ccgttggtgc cgccgaagcc
    18961 gaatgagttg gtcagcgcat attcgatgcg agcctgacgg gcgaaattcg ggacgtagtc
    19021 gagatcgcac tccggatcgg ggttgcacaa attgatggtc ggcggcatta cctgatcccg
    19081 cagcgccagc accacgatgc cggcctcgag gccgccggcc ccgccgagca ggtggccggt
    19141 catggatttt gtggaactga ccgccacctt gcgggcgtga tcgccgaaag tgcgcttgat
    19201 cgctatggtt tcgatgcggt cgccgaccgg cgtggaggtg ccgtgggcgt tgatgtaatc
    19261 gatctgatcg aagcggattt tagcgtctcg aatcgcgttg cgcatcacgc ggtacgcgcc
    19321 gtccccgtct tcggaagggg aggtgatgtg gtgcgcgtcg gcgctcatcc cgtagcccac
    19381 cagttcggcc aagatcggcg cagagcgccg tttggcgaaa tccagttcct ccagaatcag
    19441 aatgccagcg ccctcgccca caacaaagcc gtcgcgatcg gcgtcgaagg gccgggaagc
    19501 ggctcgcgga tcgtcgttgc gcgtcgagag cgcccgcatg gccgcgaagc cgccgatccc
    19561 catcggagtg atggtcgcct ccgacccgcc gcagatcatg acgtctgcct cgccgtattg
    19621 aatcaggcga aaggaatcgc cgaccgcatg ggcgctggtc gtgcaggccg tggcggttgc
    19681 cgagttcggc cccttcgctc cggtgcggat agacacctgc ccggacgcca gattgataat
    19741 ggttgcggga ataaagaaag gggaaatctt tttgggcccg cccgacagca gattggcatg
    19801 ttcgcgttcg atcacgtcga agcctccaat cccgctgccg atgtatacgc cgaccctctc
    19861 ttcgatctcc ggggttacgt tgagaccgga cgccttcaga gcgaattcgc tggcggcgat
    19921 cgcaaagtga atgaagcgcc ccattttttt cacttctttt ttttcaatat attggaaggg
    19981 atcgaagcct ttgacctcgc cggcgatctg ggtggagaaa tcggaagcgt cgaacgaggt
    20041 gatgcggccg atgccgctct ggccttccgt gatcgccttc caagtctgct ccgtccccac
    20101 gccgaccgcg gagatcaggc cgaccccggt gacaacaacc cttcgcaaac aggtctcctc
    20161 tctggccggg ggtaagtccc ttcagccata gtagcggatc gggcgcgccg gtgagcgcgg
    20221 ataaaatcaa acgcaactac gcgccggaat ccggccggcg gccgaacgcc gcggcgcgac
    20281 tctacttcac tttggcgtgg ctctgaatgt agtcgatggc gcccttgacg gtggtaatct
    20341 tttctgcgtc ttcgtccggg atatcgatct caaaagcctc ttcgaaggcc atcaccagtt
    20401 ccacaatatc aagggagtcg gcgcccaggt cgtcaacaaa ggaggcggcc ccatcaacct
    20461 gcgcctcgtc aacgccgagt tgctccacaa tgatctgctt taccttctcc tcaacggaca
    20521 tgcatcctcc aggcgaattt cgaaactact gattctagcg gggccaatcc tgaggcgcaa
    20581 ccggcatcgg ggccgtctct tggatcacca aaaatccccg caacagcgtt ggcgggctgc
    20641 tccccggacg tttcccctcc ggagcataga acaacgctat ttctcacgcg tggcgggcct
    20701 tgttttctga tttcctgaac gccgcttcgt ttggttcgag tgaatcaaca aatcgagagc
    20761 atgcagattc tgaaggaccg ctgcgatggc gacccggtcg ccggaatcga gaatacgatc
    20821 caggatttgg tgacacgcct ccctgtcatc cgagccgctg tccttgctgc gggacccgtc
    20881 gaggggcttc tgagaggccg cggaaactgc cgccgcggcc ccggagatcc ggacttccct
    20941 cggctgaaaa actctccgca cctgccacgc ctcgctcgac aactccaatg caatgtcggc
    21001 caaatcgtgc tcggcggcaa tcgacttcag cctttcgatg atctcgcgag gcacgcgttt
    21061 tccgccttcg taattctgga tcgaagagta ggagcagccg agcgcttggg caagcgcgga
    21121 ctggctccag cccagggccg tgcgcaattt ttttagcggg ctctctatag tttccacaga
    21181 aaagccgatc tacttacgcg taagcctacg catatgcctg gcggcagcgt ggcaaatggc
    21241 atgttatcgc acctccgagg tctccggcgc acggcggggc cgtggctctg ggcccagttt
    21301 agggcaggaa aggccccagg acggcaggcg ccgcctcccg gcagcggaaa aacaacgcga
    21361 ggagatcaga gtatgacaat caaagaccgt cttgatcagg aataccggct gctccaggtg
    21421 atcgaacgtg ttcgcacaca gcgggggaac ccgtatattt tttcgaatgc agaacgaaag
    21481 gtcgtgaaca cggaacttgc ccgtttggaa cgggaaatgg agatcatctg gttcacggag
    21541 ctcgtgatcg agatgtcaag ccgtctcact cccgccgaaa tcgagcgagt ggttgggcgt
    21601 ctcgcgggtt ggatcgagac ttgctgaggc ggcgggtaaa ggccgtagac cgccggaggg
    21661 aatcttcgag taaggagcct gcccatatgc tcagggaaac aacgagcgcc cagaggtttc
    21721 cgctgattgc aatcgatcca ggccacggcg ctggctgggt aaagccgaac gtcctggatg
    21781 agggcgcggt cgcttatcac aagggggcac gatacgccga acaggagctc gcgctgcgct
    21841 acgccatcgc cctggacgag gagttacaca aacggggcgt gaacacagtc ctgactcgaa
    21901 ctgattcacg aaccaactcc cctccacagc gccggaagga gctatgcagc ggcgcgagcc
    21961 tggcgctctc gctgcatttc agcaagagtc tgaaccccaa agatcgcggc ttcgaactgg
    22021 cggcgccgcc ggagctggaa gccctgagcc aggcggtccg gcaggccctc gaaatgtggc
    22081 tgccttgccg ggatgtctcc gcagtcagca gcagccgaga ttgcgtgcga gcggtgtacc
    22141 tgggatgtcc cagcctggag atccggcccg gcgttcttac ctcgtgccag gacatggaac
    22201 tgatcaccag cgcttactgg aaggtgcttt ttgcggaaag gatcgcgcaa gcggtggatc
    22261 aacattttgg atttgcctcg tgcgcccggg ccgccgcacg ctgagtaatc caacggccga
    22321 aaagccgagc gaaaccggcg gagagcggga aaagagcgaa caaagagctt gcctgctttc
    22381 ctcgggacct agcggtatct gcgatggacc gccaacacac ggccctgaat gcgcacgccc
    22441 tcgagcggca cgatgatcgg ctgcatggcg gaattcgccg gctgaaggcg cgccatgccg
    22501 ttcggctcgc gataaaagcg cttgagggtt gtctctgtgc cctccaccag ggcgacgaca
    22561 atatcgccgt cgcgaacttc ctccgtgcgc tgaaccagca cgtagtcgcc gtccatgata
    22621 tggtcgtcaa tcatggactc gccgtgaacc tggagcgcgt atacgttggg agcgcccaaa
    22681 aaatcgccga atgagagggt ctcgggattt tcggcggcct caatcggctt gcccgccgcg
    22741 attcgcccct gcaaaggcaa ttcggcggca aacgccggcc gcagggcgcg ctcctggcgc
    22801 tgccggcgct ggtcgctgta gtattgcgga cccagatcaa tcgagcggct ctggttgatt
    22861 ccccgtttca aatagccctt tgtacacaac gtcgtcagat gtttgtgaac cgtggctagt
    22921 gacgccagtt gcatagcctg cgcgatctcc tcaaagctgg gactgtatcc gtgcgcttgg
    22981 atgaaaccgg cgatgtaatc cagaacttgc cgctgccgtc gagtcagagc caaagtcgcc
    23041 tcctggaagc gcctcttatt gtaggcgatc ctattctagg cgaagaaaag gggaaaatgc
    23101 aagcctgctg tgcgactcgt ggttgcgccg ggtcccaggc gccccgcccg cagggcgtgg
    23161 cccgaaccga gagacatcac attcgccccg atgtctcggc gccacgcaaa agcagcgcac
    23221 cttagttgtg aaacgccacg ccagcgcccg ctgcggcatg agagttttca tcgccacgca
    23281 gtccgccagc ttcgatcccg gcggcgttgc cgggccgctt cggcacaggg aaagcaggat
    23341 cgctcaaggc cggcggctgt tcgcctacgt cacaactttc tcacaatgcg gaaaagcgat
    23401 catgatacgc ggccccgccg cctgagggcg tagatcgatg ctacgaacaa tctgggagac
    23461 agccgggaaa gacgctcccg gcctccggct atttcgtgct ctggttcatt tcgccggctg
    23521 tgcggttcca atttttttgg gcctcgcaac cggcgagcgc cgtttttccg cgccttttcc
    23581 gtgtacgtga aaatcgggta aactgcttta gatctccggg aggctgtgat gggaacgata
    23641 gaaagccggt ggcgtcaatc cctgacgagg cgccgcgccc ttcaggggct ggccgcgttt
    23701 ctcgccagct ctccactgct cgattcccag caagacccgt ttcgggacca cagccgggtt
    23761 cccgcgctca acgaactgct gacctctttc gacttcgagc cggttttctt tgcaaaggag
    23821 ccgcgggcaa tctacgatta cacctcctat ggcgcagaga gcgagttcac att
//
LOCUS       DASREK010000276         7167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1120894, whole genome shotgun sequence.
ACCESSION   DASREK010000276 DASREK010000000
VERSION     DASREK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120894"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFA54_14950"
     CDS             <1..244
                     /locus_tag="VFA54_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52163.1"
                     /translation="ADLLAAVRAFDRRILNADISMGLERMDRGLQRVQRVRCVLDRNE
                     FLDPDEERRVLAEELAGHLRERMLYGVELALQLEWQ"
     gene            241..1227
                     /locus_tag="VFA54_14955"
     CDS             241..1227
                     /locus_tag="VFA54_14955"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HZS52164.1"
                     /translation="MTQAPPASSVMPDFSHAAYGFHHTVDSALEVLAGCGVSPSRITL
                     RMAGRGWPTYWVVGQSPPPGAPLGPDASIALSVAGLGFFHHLPTGMWDKGSEAEPGTQ
                     EILEILDDPIQKAAFWIRQGARLFDVSPDDPGACSRWISLFGLTADDWPPETWYKLAL
                     LLPSLHRIAGRVEGVKLALELLLDLPLYEIRKRRSYSYLEDSDLSLLGWQGARLGVDC
                     IVGDRMEDLARLIFVIGPVTLSTYYEYQKDEGRRRLEAVLRLCAACHQNYSVDWRVLH
                     PERPPRLGLETENARLGVNSHLGSEKPAWLARARKRKTPAFASQDSMGGERN"
     gene            1369..3201
                     /locus_tag="VFA54_14960"
     CDS             1369..3201
                     /locus_tag="VFA54_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52165.1"
                     /translation="MDPNDIKLSSVNWEHGMLLTPEHFLRQERYIDSAVLWAIRYCTG
                     GFGLVGGGPRLPESERGAVRHDPVITVDQDDEAVSVSVSQCRGLTPAGCIIDVDPEHP
                     LHRRFPKADLEGVSETGVYIVCDPQEKQISDGVLDEFNPQMKTERRPAYGLKLRVDAE
                     DAPYSIIIARLRRPEYGAGYEKDAQFIPPCVSLASYSELTAGWRKILEQAAALSERYA
                     ALYRAMREYLTLFTERGIETDLDRETMDFVGRMVAAIENCIYEALDPVQPPERFFSQL
                     RRFFHGSAVYLDLSPPVRQYFDQMIETGETEFISLLEQQKQMLQVRRKWEIHDDLGVE
                     VRSALQSFSALERLERALEGKYIDFRISPALEGMNFVFDRGGKVLYKLAAKPARVQGF
                     ADELTMFFAQLRLEGREKYRLILVGEKNATFEAGTKITAEIKINEGAGFKRQPVIVAC
                     TSKSVEQRNFEFDFEAPDVPTITDLRVSIPAYHPIRTALLFVRHRFYAGRVDQAAPAA
                     PPAAAARPEPSLRPAAEAPRVRVATEDLIEQPRMPAAPGPQPQGYPPTRETPRSPDRY
                     DTNRTGEAPAAPWESGRGVPPRPERPRDPGPEGAPAPPRRRRLE"
     gene            3229..5871
                     /locus_tag="VFA54_14965"
     CDS             3229..5871
                     /locus_tag="VFA54_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS52166.1"
                     /translation="MAINLDKISNELEDALERARLLAEERKQSLITPFHLLYVLLDGE
                     SALAAMLEKAGVACAPLLDTLATDLNKVDGMQKLQPGKRPTASRALRELLEKSFEKMA
                     ERGAEMAEPIDFVLAAVEFSEEQLKGNLRSAGLTKKTVEKAVQSREETGETLGERPSP
                     VAAAAKPGGTKILERFGRDLTAAAAAGELMPVVGRDEEIRRVIQTLLRKTKNNPVVVG
                     DPGTGKTAVAEGLAQRIAAGDVPDSLKKCRVVSLDLTSMVAGAKYRGEFEERIKSVVD
                     EVRARKGEIILFLDELHTLVGAGGTEGGMDAANILKPALARGELRCVGATTYDEYRER
                     IEKDGALARRFEVVPVKEPDDAAMMVILRGIRSRYEAHHGVRLTEDALQAAVKFSRRY
                     IRNRFLPDKAIDIIDEATARIRMQKESKPNIIDEQERLLIRKKAELEALQGTAKTHAE
                     KKAVAQLEKEIAELEPKVEKLVSQWKAQKEASDNLQKTKQAIEEQTALLQKAEAEGNV
                     AKAAEIRYGSLKYLEQQREDLEKQVAANSAGAAMIPDEVHVEHIAEVVGERVGVPIQR
                     MLESERDRLLKMEERIAERVFGQDEAVRAVAEAARRMRADLQPDRKPNSFLFVGPTGV
                     GKTELAKALAEALFDDDTALIRIDMGEYKDKSSVAGLIGSRPGLVGSDEGGFLTEQVR
                     RSPYSIVLFDEVEKGAPEILDLLLGVLDEGRLTDAKGRFCDFSNTLIIFTSNLGVREA
                     VDMTDDPEKRKDIIMEVVKGSLRPELYNRISQVICFNSLTEAELERIVNTHIGKLRRK
                     LAEDRGIHLEVSPEALAYLAKQSYDPAYGARPVGRTMQQLVLSPAATAILAGEVGPNQ
                     TLHVGYTEQDGLTFSVEDTAAAPL"
     gene            complement(5990..>7167)
                     /locus_tag="VFA54_14970"
     CDS             complement(5990..>7167)
                     /locus_tag="VFA54_14970"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52167.1"
                     /translation="NYLRIAQIYRQKRAFAQAHAALQKAAALAPADMVEVPYDEVLLL
                     ESEGKLDQAVAGIQKLIDSSAKSNPADYSPQDRRNRAFFFEKLGQLERTRENYAAAEK
                     AFRAMAEADPESAPRASIQVVETFRSARDFKRALAESEAALKKYPQDRSVAIERASVL
                     ADNGEAKQGAQIIRGFLKNGPDDLDLLLSMAQVYEKGKLFSEAKDCIEKAQTYAQTKE
                     QKKAIYFSYGALLERQKKYDDAEKQFRLALEIDPQDAATLNYLGYMLADRNVRLDEAY
                     TLVKKAIELEPDNGAYLDSLGWVYYRQNKLDLAEELLLRAVQKTGRDATVHEHLGDVY
                     FKEGKIRLAQEQWQRSLREWDLSPKSEFDSEDVAKLRKKLDDVKVRLAREQNNGKQR"
BASE COUNT         1414 a         2146 c         2267 g         1340 t
ORIGIN      
        1 tgcggatctg ctggccgccg tgcgggcctt cgaccggcgc atactcaacg ccgacatctc
       61 catgggcctg gagcgcatgg accgggggtt gcagcgcgta cagcgcgtgc gttgcgtgct
      121 cgatcgcaac gaattcctgg accccgacga ggagcgccgg gtgctcgccg aagagctcgc
      181 cggccacttg agggagcgca tgctctatgg cgtcgagctg gcgctccaac tggaatggca
      241 atgacccagg cgccgccggc cagctccgtt atgcccgatt tttcgcacgc ggcctacggg
      301 tttcaccaca cggtggactc cgcgctcgaa gtgctcgccg gttgcggcgt ctcgccgtcg
      361 cgaattacgc tgcgcatggc cggccgcggg tggccgactt attgggtagt gggccagtct
      421 ccgccgccag gcgccccgct gggacccgat gcgagcatag cgctgtccgt cgctggcctc
      481 gggttcttcc accatctccc caccggcatg tgggacaaag gcagcgaggc ggagccgggg
      541 actcaggaga ttttggaaat cctggacgac ccgatccaaa aagcagcctt ctggatccgg
      601 cagggggccc ggttattcga cgtttcgccc gacgatcctg gcgcctgctc gcgctggatt
      661 tcgttattcg gcctcacagc ggatgactgg ccgcccgaga cctggtataa gctggctctt
      721 ttgctgccca gcttgcatcg aatcgccggg cgcgtagaag gcgtcaagct cgcgctcgaa
      781 ctcctgctgg atctgccgct ctacgagatc cggaaacgta gatcctacag ctacctggag
      841 gactccgacc tcagcctgct gggctggcag ggcgcccgcc tgggcgttga ttgcatcgta
      901 ggcgaccgca tggaggatct agcgcgactg atttttgtga tcggccccgt gacgctgagc
      961 acctattacg aatatcaaaa agacgagggg cggcgcaggc tggaagccgt gcttcggctt
     1021 tgcgcggctt gtcatcaaaa ctacagcgtg gattggcgcg tgctgcatcc cgagcgcccg
     1081 cctcgtttgg gcctggagac cgagaacgcc cggctgggcg tgaactccca cttgggcagc
     1141 gagaaaccgg catggctagc acgcgccagg aaaagaaaaa cgccagcctt tgcgagccag
     1201 gacagtatgg gcggtgaacg caactgactg ctctgtcggg attggcgctc gccgatctcg
     1261 ctaccgggcg ggaggggcgc gggcctcgac aggccttgcg ttttggatcg gcggaggccg
     1321 gatctcctgc ttgagccggc ggcaagggcg tcggcgaagg aactacagat ggatcctaac
     1381 gatatcaagc tgagctcggt gaactgggag catggaatgc ttctgacgcc ggagcacttc
     1441 cttcggcaag agcgttatat cgactcagcc gtgctgtggg ccattcgcta ttgcaccggc
     1501 ggtttcggcc tggtgggagg cggcccgcgg ctgcccgaaa gcgagcgggg cgcggtccgg
     1561 cacgatccgg tgatcacggt ggatcaggac gacgaagcgg tctccgtttc cgtctcccaa
     1621 tgccgggggc tgacgccggc cggctgtatc attgacgtgg atcccgaaca tccgctgcac
     1681 cggcgttttc cgaaggccga tctggaggga gtgtcggaaa cgggcgtcta tatcgtttgc
     1741 gatcctcagg agaaacagat tagcgacggc gtgcttgacg agttcaatcc gcagatgaag
     1801 accgagcgcc gccccgctta cggcttgaag ctgcgtgtgg acgccgagga tgctccctac
     1861 agcatcataa tcgcgcgcct gcgccggccc gaatacggcg ctggctatga aaaggatgcc
     1921 cagttcattc caccttgcgt ctctctcgcc agctacagcg agctaaccgc aggatggcgc
     1981 aagatcctgg agcaagccgc ggccctctcg gaacgctatg ccgcgcttta ccgggccatg
     2041 cgcgaatatc tcacgctgtt cacagagcga ggcattgaaa ccgacctgga ccgggaaacg
     2101 atggactttg tgggccgtat ggtggcggca attgaaaact gcatctacga ggcgctcgat
     2161 cccgtgcagc cgccggagcg gttcttcagc cagttgcggc ggtttttcca cggctctgcc
     2221 gtctatttgg atctgagtcc tccagtgcgg cagtatttcg atcaaatgat tgaaacgggg
     2281 gaaaccgagt tcatttctct gctcgagcaa cagaagcaaa tgttgcaggt gcgccgcaaa
     2341 tgggagattc acgacgatct gggcgtggag gttcgctcgg ccctccagtc gttttccgct
     2401 ctggagcgtc tggagagagc gctcgagggc aagtatatcg acttccgcat cagtccggcg
     2461 ctcgaaggca tgaacttcgt tttcgacagg ggcggaaagg tgctttacaa gctggccgcc
     2521 aagcccgccc gggtgcaggg cttcgccgac gagttgacga tgtttttcgc ccaactgcgc
     2581 ctggaagggc gagagaaata ccgcctgatt ctcgtcggcg aaaagaacgc tactttcgag
     2641 gcgggaacca agatcaccgc cgagatcaag atcaacgagg gcgccggctt caagcggcag
     2701 ccggtaatcg tggcctgcac atccaaatcc gtcgagcagc gaaactttga atttgatttt
     2761 gaggcgccgg atgttccgac gatcactgat cttcgggtga gcatcccggc atatcatccg
     2821 atccgcaccg cgctgctgtt tgtccgtcac cggttttatg cgggacgagt ggatcaggcc
     2881 gcaccggccg cgcctcctgc ggccgccgcc aggcctgagc cttctctgcg gcccgcagcc
     2941 gaagcgccgc gcgtgcgcgt agcaaccgaa gacctgattg agcagcctcg catgccggcg
     3001 gcgccgggac cgcagccgca gggctatccg ccgacgcgcg aaacgccccg cagtcccgat
     3061 cgatacgaca caaaccgaac cggcgaggcc ccggcagccc cgtgggaaag cggccgcggc
     3121 gttcctccgc gaccggagcg gccccgcgac cccggcccgg aaggcgcccc tgcgccgccc
     3181 cgccggcgcc gcttggagta gaaacagcca ttcggagtaa gagcagccat ggccattaat
     3241 ctcgataaga tttcgaatga actggaagat gcgctcgagc gggcccgcct gctggcggaa
     3301 gagcgcaagc aatcgctgat cacgcctttt cacttgctct atgtcctgct ggacggcgag
     3361 agcgctctgg ccgctatgct cgaaaaagcc ggcgtggcct gcgcccccct gctggatacg
     3421 ctcgccacgg atctcaacaa agtggacggg atgcagaaat tgcagcccgg caagcgcccg
     3481 acggcctccc gcgccctgcg cgagctgctc gaaaaatctt tcgagaagat ggccgaacgc
     3541 ggggccgaaa tggccgagcc gatcgatttc gtattggcgg ccgtggaatt cagcgaagag
     3601 cagctcaagg ggaatctgcg cagcgcgggc ctcaccaaga agaccgtcga aaaagccgtc
     3661 caaagccggg aggaaaccgg cgagactctc ggcgagaggc cttcccccgt tgccgctgca
     3721 gctaaacccg gggggacgaa aattctggag cggttcgggc gcgacctgac cgcggctgcg
     3781 gcggcggggg aactgatgcc cgtcgtcggc cgcgatgaag aaatccggcg cgtgatccag
     3841 acgctgctgc ggaaaaccaa aaacaatccc gtagtagtgg gcgatcccgg cacgggcaaa
     3901 acggccgtgg ccgaggggct cgcgcagagg atcgccgccg gcgacgtgcc agactcgttg
     3961 aagaaatgcc gcgtggtttc gctcgacctc acttcgatgg tcgcgggcgc caaataccgg
     4021 ggcgaattcg aggaacggat caaaagcgtg gtggacgagg tacgcgcccg gaaaggggaa
     4081 atcattctct ttctggacga actgcatacc ctggtcggcg ccggcggaac agaaggcggc
     4141 atggacgccg ccaacatact caagccggcc ctggctcggg gagaattgcg ctgcgtggga
     4201 gctaccacct acgacgagta ccgtgagcgc attgaaaaag acggcgcact ggcccggcgc
     4261 ttcgaagtag ttcccgtgaa ggagccggat gacgccgcga tgatggtgat cctgcgcggc
     4321 atccggagcc gctacgaggc tcatcacggc gtgcggctta ccgaagatgc gctccaggcg
     4381 gcggtgaaat tctcgcgccg ctacatacgc aaccgctttt tgcccgacaa ggccatcgac
     4441 atcatcgacg aagccacggc gcgcatacgg atgcagaaag aatccaagcc caacatcatc
     4501 gacgagcagg aacggctgct gattcgcaaa aaagccgaac tggaagccct gcaaggtacg
     4561 gcaaagacgc acgcggaaaa gaaagccgta gcgcagctcg aaaaggagat cgcggagctt
     4621 gagcccaaag tggaaaagct cgtcagtcaa tggaaggcgc agaaagaggc ttcggataat
     4681 ctgcaaaaaa ccaagcaagc catcgaagag cagaccgctc tgctgcaaaa ggccgaggcg
     4741 gagggcaacg tcgcaaaagc cgcggagatc cgctatggct cgctgaaata cctcgaacag
     4801 cagcgggaag atctggaaaa acaagtggcg gccaactcgg cgggcgcggc gatgatccca
     4861 gacgaagtgc acgtcgagca catcgctgaa gtcgtcggcg agcgcgtggg cgtgcccatc
     4921 cagcgcatgc tggaatcgga gcgcgaccgg cttttgaaga tggaagaacg tatcgcggag
     4981 cgggttttcg ggcaggacga ggcagtgcgc gcggtggccg aagccgcgcg ccgtatgcgg
     5041 gcggatttgc agcccgaccg caaaccgaat tcttttcttt tcgtgggccc caccggcgtg
     5101 ggtaaaaccg agctcgccaa ggcgctcgct gaggccttat ttgacgatga tacggcgtta
     5161 attcgcattg acatgggtga atacaaggac aaatcgtcag tggctgggct gatcggctcc
     5221 cggccgggtc tggtcggttc tgacgagggc ggtttcttga ctgagcaggt tcgccgctcg
     5281 ccttattcca ttgtgctgtt cgacgaagtc gagaaaggcg cgcccgaaat tctagacttg
     5341 ctgctgggag ttctcgatga gggccggctc acggacgcca aaggccgttt ctgcgatttc
     5401 tccaatacgc tgatcatctt tacgtccaac ctgggcgtgc gggaggccgt ggatatgacc
     5461 gacgacccgg aaaaacgcaa ggatatcatc atggaagtcg tcaaaggcag cttgcggccg
     5521 gagctttata accgcatttc gcaggtgatc tgcttcaact cgctcaccga ggctgagctt
     5581 gagcggattg tcaacaccca catcggcaag ctccggcgca agctggccga ggaccgcggc
     5641 attcacctgg aagtttcacc cgaggctctg gcctatcttg ccaagcaatc ctacgatccg
     5701 gcctacggcg cacggcccgt aggccgcacc atgcagcaac tggtgctctc gcccgccgcc
     5761 acggcgatcc tggccggcga agtcggcccc aatcaaaccc tgcacgtcgg ctatacggag
     5821 caggatggct tgactttcag cgtcgaggac acggcagcgg ccccgctgta agagcaaggg
     5881 ccttcaggta tatctgacag gaagttgatc ccggcctgcc tctgcggcga taccggatca
     5941 cgttcgggtt gctccgggca aggggaagtc ccggaagcaa tgacctgaat cagcgctgtt
     6001 ttccgttgtt ctgctcgcgc gccagacgga ccttcacatc gtcgagcttc ttgcgaagct
     6061 tcgcgacgtc ttcggaatca aactcggact tcgggctcag atcccactcc cgcaacgagc
     6121 gctgccactg ctcctgtgca aggcggatct tgccctcttt gaagtagaca tcgccgaggt
     6181 gctcgtgaac cgtggcgtcc cggcctgttt tctgcaccgc gcgcaaaaga agttcttcgg
     6241 cgagatcaag cttgttctgc cgataataaa cccagcctag gctgtcgagg taagcgccgt
     6301 tgtcgggttc cagctcgatg gcctttttaa ccagggtgta ggcctcgtcg agacgtacat
     6361 tgcggtcagc cagcatgtag ccgaggtaat tcagcgtcgc cgcatcctgc gggtctattt
     6421 ccagcgccag acgaaactgc ttctcagcgt cgtcgtattt cttctggcgc tcgagaagag
     6481 ctccgtagct gaaatagata gccttctttt gctctttcgt ctgggcgtac gtttgcgctt
     6541 tttcgatgca gtctttcgct tcgctgaaga gctttccttt ttcgtacacc tgggccatgg
     6601 aaaggagcaa gtccaggtca tcgggcccgt tcttcaggaa gccgcgaata atctgggcgc
     6661 cttgcttggc ttcaccgtta tccgccaaca ccgaggcgcg ctcgatcgcc accgaacgat
     6721 cctggggata cttcttcagc gccgcctccg attcagccag agcacgtttg aaatcgcggg
     6781 cgctccggaa cgtttccact acctgaatgc tggcccgcgg ggcgctctct ggatcggctt
     6841 cggccatcgc gcgaaacgct ttttcggcgg cggcgtaatt ttcgcgggtc cgctcgagtt
     6901 gcccgagttt ttcgaagaag aaagcgcggt tgcgccggtc ctggggggag taatcggcag
     6961 ggttgctttt ggctgaagaa tcaatcaact tctggattcc cgccacggcc tggtcgagct
     7021 ttccttcgct ttcgagcagc aggacttcgt cgtagggcac ttccaccatg tcggccggcg
     7081 cgagggcagc ggctttttgc agcgcagcat gcgcttgcgc gaacgcgcgc ttttggcggt
     7141 agatttgggc gatgcgcaga tagtttt
//
LOCUS       DASREK010000277        11009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1950899, whole genome shotgun sequence.
ACCESSION   DASREK010000277 DASREK010000000
VERSION     DASREK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950899"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(420..701)
                     /locus_tag="VFA54_14975"
     CDS             complement(420..701)
                     /locus_tag="VFA54_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52168.1"
                     /translation="MTLLDLRRYAVSNRLRIRFALPSGVECCMDEHGILKIPSLRSAP
                     DFNVETLLPSVENFSLDPIQEGRRSRNVSREQLELLCSGGGAKEQEHED"
     gene            complement(963..1637)
                     /locus_tag="VFA54_14980"
     CDS             complement(963..1637)
                     /locus_tag="VFA54_14980"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZS52169.1"
                     /translation="MRSVLSPEAAIVQVSQNGLVCSASAAALDALRAAFDRSHCIRLR
                     QFVAPGLLDLLQRHIDRAEFYDRLHDGIGLELCMVDNAIALELHLLTNRPDLFRIVER
                     ITGCGRIGSFEGRVYRMKPGGGHYDSWHGDVGDERMIGMSVNLSTEVFSGGLFQLREL
                     APQSPILCEVANTGFGDAILFRISRQLCHRVTPMEGSAAKTAFAGWFKAQLPMHPLLR
                     KCAEGD"
     gene            complement(1634..3232)
                     /locus_tag="VFA54_14985"
     CDS             complement(1634..3232)
                     /locus_tag="VFA54_14985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS52170.1"
                     /translation="MRCRRFVILLAFAAAASAGLLVYSLTVACYGDEGFHLLAARLIR
                     AGRRPYVDFFYQHPPLYPYLNAFWMGIFGDGWRSVHALSSLLASGGILLAAFCVFSRL
                     TDPLWQLPAAIAAALLMASQPLVIQFGGIGQPYGLCLFASMAAFWLAASAAAHPRRAC
                     FAAGFCAGAAAGSSLLAVPVGGVLFVWLLRDAGKGKRMASGALFLAGAMVCFLPLLYW
                     VWQAPEQTFFDVVKYHAFYRSSAPASDLRRDFEVLKGWLDSPQAVLRALLAAVSLLYL
                     GGRPDAPMRREAYLCAWLAGALAVWVAVAHPALPQYFILLAPFVSILDALGLYAIGSR
                     LWAPEHAAWPASLCLCLLAAALAKPVYDGRRGQGYRWSDWERVAQEVNRVTPESGLLW
                     APDIIYFAARRLPPAGLENSNSLALRLPPALSSRLHIIPEAEADQWVANGRFDTVCAC
                     VGMRVSANPDPPLGHGLPGPYKNQLQIQDCYIFWGSSRGAGRPGAEQPWPGSPASNRF
                     DIYWQHAGFRVPPLQDQPEARRAR"
     gene            complement(3211..4155)
                     /locus_tag="VFA54_14990"
     CDS             complement(3211..4155)
                     /locus_tag="VFA54_14990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZS52171.1"
                     /translation="MRDAAPVSAVIPAWNAEDFLAEAVQSVQSQSLPVSEIIVVDDGS
                     DDGTASVAGELGVQVLRQPHRGVAAARNTGIRAARQDWIAFLDADDIWEPEKIAHQWN
                     ALQKHPSAGMVSCDLCIVEDGQVVSPSSLDQTNPDLEALYFPQVPHDFLACQMNYLPS
                     GVLVRRDAILSVGLFDESLHYNEDFECFLRVLACRPLIMVMRPLVRHRRHARNASRNT
                     PKMALSYSDVVRRMRRNPERYPPGAGRACRRESLEMLSCTGRALAVQGRHREARQLLA
                     RRLREQFDFRLFVWWSFTWLSPRMLQKVIHLRCAAGAS"
     gene            complement(4177..5685)
                     /locus_tag="VFA54_14995"
     CDS             complement(4177..5685)
                     /locus_tag="VFA54_14995"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS52172.1"
                     /translation="MLISGIAPGVKIGIEYGELDPLYAPDLDCRLLFLGEHLQPLVHA
                     YENLARCFAESGDPSSLAARLESSKEITKYYEIRRNGRLSAVLRDSVFRQQGPFDDRK
                     LRLKFYRAGKSVSHPLPRRRLRALGRLWPLLGSNRPQEEIAALLKSNLPADDAAWAAE
                     LLALLETHQLLDRQPRDAGFLRRPAPRVTFMGHTSLLFQSGRSAVLMDPLLPTEAAYA
                     RSALAVQQIRLGAICCSHHHWDHCNFQTLMGFDKSTPVIIPRVERPSAFNPPIAGALQ
                     MLGFTDIREARLWEPAPIDDIEVIPTPFYGEQDEPGAECDHYTYVLRTKGLTVYGGVD
                     CFRDTFGETVDLMEEIRERYQPQIAFLPISKSVFSYRYGGANGFCRYLDQELVTQSFQ
                     YTAGPEEAAEWVSRLRAPVVAPYAIFTFSRWSMPAVVAQFHDALRARGLGERLYPLRP
                     LDSLDLSALAGSRPAGRSAAVTLFRAGAHIRRVSGSPPYRLAKRVMKSLHLA"
     gene            complement(5724..6560)
                     /locus_tag="VFA54_15000"
     CDS             complement(5724..6560)
                     /locus_tag="VFA54_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52173.1"
                     /translation="MLNLVIVAEGSHSVPQVDWSDPGVEAWHDNDGALCAYGYSLDGR
                     PWIDWPGVARFCLDGPDQGTVTAIPWQPARRWAIEEVFRRSILPLALQARGREALHSS
                     AVLMPQGIVGTCGVSESGKSTIAFGLSCRGYPLWADDAVAFDASGDRVTGIPLPFRIR
                     LRQPSAAFFGENSSRLERSVRCETEPRPLAALFVLNRTSSARNGGICEIQRLHSARAF
                     AAVLTHALCFSLLDKSRKRSMFHHYMNLITKVPVFELRFQPDLNGLPALLDAIEKKVG
                     EI"
     gene            complement(6860..8776)
                     /locus_tag="VFA54_15005"
     CDS             complement(6860..8776)
                     /locus_tag="VFA54_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HZS52174.1"
                     /translation="MSLFPTSAPLVGDPEIRRELPGVWLASLGGDPRAEAQHAGWTIV
                     TSDGRSRALSLLAAGEQAQPSAAQARSCGVIFDGVLYNRAELGRELERTLDEAESDAS
                     LALRSYLRWGEDALRKMKGHFALLLWDSESDLSLCARDPVGIYPLFYAETTRELLLST
                     SIEALLRHPRVSRAFNRPAIADQLRFRFADLHETVYESVKRVPPGHVLRVAHGQKQVY
                     RYWDPASPNGRVNWLREDELDQFDQLLDQAVTRCLQFGPAGIFLSGGLDSVTVAAVSA
                     EKCRDQGLPDPLALSLVFPHPEANEESVQRSVAEGLGLQQVVIPFEEAVAPAGLVSAA
                     LEMSSRFSMPLMNTWLPAYHHLGEAGRQRGCRTILTGTGGDEWLTVTPFLAADLLRRA
                     DVVGFYQLWRSMHQSVRRSPAAVFRLLLWKCGLKPLLIQALRQAAPEPLLRARRSRSA
                     RRVPAWLAPDAAVRRELICRGEQAAPESESDKAGFYIQEMRSALDHPLVSWELEESFE
                     NARSMGVRMLHPFWDSDLVDLLYRIPPLLLNKGGRSKGLVRESLARRFPDLGFERQKK
                     VEATAFFWSVILTKGAQTWHELGGPRALAKLGLVDPQILTAEIDEIVAKNCGPEAYRI
                     IHVLTYEAWLRPRL"
     gene            complement(8757..9068)
                     /locus_tag="VFA54_15010"
     CDS             complement(8757..9068)
                     /locus_tag="VFA54_15010"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HZS52175.1"
                     /translation="MTARNTEDGISALPLRAEPNAEVVFQRVGDTVVLLHLGTDKFYE
                     LNQTGARFWELLAAGNGPAAVHEQMVREFDVDPAELAREVNQLLASLAKEDLIHVVVS
                     H"
     gene            complement(9200..10183)
                     /locus_tag="VFA54_15015"
     CDS             complement(9200..10183)
                     /locus_tag="VFA54_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52176.1"
                     /translation="MAEAFQELGEAASARYCMARAIERGPHSPYVLLRAGNLDLELGE
                     TQEALRRLARVLEIVPDYDPVIFASYARFVGSAREVLREGIPNNRRAAQSYFRYLLRD
                     GGESDLQLAWDWLSARSFTDVALAGEYADVLLKRRNYESAARVWRSQFEGGMPQQTQS
                     DRAFNGDFERDFTGCRLDWRIEPAKGAQAALDRTVSYSGARSLRIRFDGTENVDYRHV
                     FEAVPLKPGRYRLQAYVRTEDLTTDEGIGLRIVDPQSPARLDVSTPSVTGTSDWKKVE
                     AVVNVPGYIGLAELQVVRRPSMKFDNLIAGAAWVDAVALLPEGRPALAQGR"
     gene            10800..>11009
                     /locus_tag="VFA54_15020"
     CDS             10800..>11009
                     /locus_tag="VFA54_15020"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HZS52177.1"
                     /translation="MHSSETVPDRNWFALQVKPRHEKAAANALRGKGFEEFLPMCTSV
                     RRWSDRWKELQTPLFPRYVFCRFHLK"
BASE COUNT         2350 a         3485 c         3227 g         1947 t
ORIGIN      
        1 tctgggcatg gctttatata ccggccggct tccccttacg gacctggctc gcttgaatcg
       61 agagctggcg caatcggcga gtcgccccta attggcgcct tccaccgcga ctggaaatcc
      121 ggcaacatca atgctcacca aagagtaggg gcaagctgct ggactttgcc ctcgcgaaga
      181 tgcctgaggc cgcgccgggc ggggccgccg gcgtggccct aggtccgact aaggacctca
      241 cccagtccgg ggcgatcccg gttattgtcc agtacacggc gctgagcagc tcgaaggtaa
      301 agacgccgac gcccggagcg acctcttccc cttcggcgcg atgctttacg ggatactcgc
      361 cggaggcgag gcgttctccg ggaacagcgc ggcgagcctg agtgccgcct tgtgtagatt
      421 cagtcctcgt gctcctgctc tttggcgccg ccgccgctgc acagcagttc cagttgctct
      481 cgagaaacgt tccggctgcg ccgtccttcc tgaatagggt cgagggaaaa gttttcgacc
      541 gagggcagga gagtttcgac gttaaaatcg ggtgctgacc ggagcgaagg aatcttcaga
      601 atgccgtgct cgtccatgca gcactccacg ccggaaggaa gagcaaatcg gatgcgcaga
      661 cgattgctaa ctgcgtaacg ccgcaaatcc aacagtgtca tgacgaagac cgggattgac
      721 tcaatgaccc accggcctcg gggaggactt cccttcccgg caacggctgc ctttcgaaac
      781 ggtgagccac gagcgagatt ctacgaaaga ctgcaagccg aaggctcggg cgccagcaac
      841 ctaaccaagt aatggcgtcc ccaacagcgg gcagcggctg gccgcaccgc gtgacctgag
      901 tgctgcgagg cgagcatccc gtctcggcgg gaaggcgttt cactcggtgg ggcgcgctcg
      961 ccttagtctc cctcagcgca tttccgcagc agtggatgca tgggcaactg cgccttgaac
     1021 catcctgcga atgcggtctt ggccgcgcta ccctccatcg gggtcacacg atgacaaagc
     1081 tggcgcgaga tgcggaacaa aatagcatcg ccgaagcccg tattggccac ctcgcaaagg
     1141 atcggacttt gcggcgccag ctcccgcaac tgaaagaggc cgccggaaaa aacttccgtg
     1201 ctcaggttca cgctcatccc gatcatccgc tcgtcgccga catcgccgtg ccaggagtcg
     1261 tagtggccgc cgcccggctt catgcgatac acgcgcccct cgaagctgcc gatgcgccca
     1321 cagccggtga tgcgctcgac gattcggaac aggtccggtc ggttagtgag aaggtgcagc
     1381 tcaagggcga tggcgttgtc aaccatgcac agttccagcc cgatgccatc atgcaaccga
     1441 tcgtaaaatt cagcgcggtc gatgtggcgc tggagtaggt ccaataggcc gggcgctacg
     1501 aactgccgca aacggatgca atgcgagcga tcgaacgcag cacgcagcgc gtccagcgcc
     1561 gcggctgagg ctgagcaaac cagaccgttc tgtgagactt gcacgatagc ggcttcagga
     1621 gacagaacgc ttctcatcgc gccctccgcg cttccggctg gtcctgtaaa ggcggcactc
     1681 ggaatcccgc gtgttgccag tatatatcga atctgttcga cgccgggctt ccgggccaag
     1741 gctgctcggc gccgggccgc ccggcgcctc gcgagctgcc ccaaaagata tagcaatcct
     1801 gaatctgcaa ttggttttta tagggaccgg gcagaccgtg accgagcggc ggatccggat
     1861 tggcgctgac ccgcatcccg acgcaggcgc acacggtatc aaaacggccg ttggccaccc
     1921 attggtcggc ctccgcctcc ggtatgatat gcagccgaga agacaaagca ggcggaagcc
     1981 gcaaagcaag actgttggaa ttctcgagcc cggctggagg cagccggcgc gcggcgaagt
     2041 aaatgatgtc gggagcccag agaagcccgg actcgggcgt gacccgattc acttcctggg
     2101 ccacgcgctc ccagtcgctc cagcgatatc cctgacccct gcgcccatcg taaaccggct
     2161 ttgcgagcgc cgcggccaac aggcacaggc agagggaggc gggccaggcg gcgtgttccg
     2221 gggcccaaag gcgcgaacca atggcgtaca gccccagggc gtccaggatg ctcacgaagg
     2281 gcgcaagtaa aatgaaatac tgcggcagag ccggatgcgc cacagctacc caaacggcca
     2341 gcgctccggc aagccacgcg cacagatagg cctcgcggcg catcggcgca tccgggcggc
     2401 cccccaggta cagcaagctc acggcagcca gcagggcgcg caatacggcc tgcggcgaat
     2461 ccagccaacc tttcagcact tcgaaatccc gccggagatc ggacgcgggc gccgagctgc
     2521 ggtagaaagc gtgatatttc acgacgtcga agaaagtctg ctccggcgcc tgccagaccc
     2581 aatagagcag cggcagaaag caaaccatcg cacccgcgag gaacaaggcg ccgctcgcca
     2641 ttctttttcc cttgccggcg tcccgcaata gccaaacgaa cagaacgcct cccacaggca
     2701 cggcaagcaa cgatgaaccc gccgccgcgc cggcacagaa tccggcggcg aagcacgccc
     2761 gccggggatg agccgcggcg gaggcggcca gccaaaaagc cgccatgctg gcgaaaagac
     2821 aaagtccata gggttgcccg atcccgccga attgaatgac gagcggctgc gacgccatca
     2881 gtagcgcggc ggcaatcgca gcaggaagct gccacagggg atcggtaagt cttgaaaaga
     2941 cgcaaaatgc tgccaggaga atgccgcctg aagcgagcag gctggagaga gcatgaacgc
     3001 tgcgccagcc atccccaaaa attcccatcc aaaaagcatt caaataggga tagagtggcg
     3061 ggtgttgata gaaaaaatcc acataaggcc tccgcccggc gcggattaac ctcgccgcca
     3121 gaagatgaaa gccttcatcc ccgtagcagg cgaccgtcag cgagtaaacc agcaagcccg
     3181 ccgaggccgc ggcggcaaaa gccaggagga tcacgaagcg ccggcagcgc atctgagatg
     3241 tatcaccttt tgcaacatcc gggggctgag ccacgtgaag ctccaccaaa cgaacaggcg
     3301 aaagtcgaac tgctcccgca gtcgccgcgc cagcagctgg cgggcttccc tatgccggcc
     3361 ttggaccgcc aaggcgcgtc ccgtgcagct taacatttcc agtgattccc tccggcaggc
     3421 ccggcccgcg ccgggtggat agcgctccgg attccggcgc atccgccgca ctacatcgct
     3481 gtaagacaaa gccattttcg gcgtgttccg ggaggcattg cgagcgtgcc ggcggtgccg
     3541 caccagcggc cgcatgacca taatcagcgg cctgcacgcc aacacgcgca agaaacactc
     3601 gaagtcctca ttgtagtgga gagactcgtc aaagaggccg acggaaagaa tcgcgtcccg
     3661 gcgcaccaat accccggaag gaaggtaatt catctggcag gccagaaaat catgcgggac
     3721 ctgcgggaag tacagcgcct ccaaatcggg attggtctga tcgagagatg acggcgacac
     3781 tacttgcccg tcctctacga tgcacagatc gcaggaaacc atcccagccg acgggtgctt
     3841 ttgcagagcg ttccactggt gcgcaatctt ctccggctcc cagatatcgt cggcatccag
     3901 aaaagcgatc cagtcttgcc tagccgcgcg aatacccgta ttgcgcgctg cggcaacccc
     3961 tcggtgcggc tgccgcagca cttggacccc tagttctccg gcgaccgacg ctgtaccgtc
     4021 atcggagccg tcatccacca ctatgatttc tgagacaggc agggactggg actggacgct
     4081 ctggaccgcc tcggcgagga agtcttcggc gttccaagca ggaatcacgg ccgagaccgg
     4141 ggcagcgtcg cgcatctcgg ttcctctggc gctatgtcac gcgagatgca agctcttcat
     4201 gacccgcttg gcaagccggt aaggcggcga cccactcact cgccttatgt gcgcgccggc
     4261 ccggaacagg gtaaccgccg cgctgcggcc ggcaggacgg cttccggcaa gcgcagagag
     4321 atccaaggag tccaggggcc gcagcgggta gaggcgttcg ccgagccccc gcgcccgcaa
     4381 ggcatcgtga aattgagcca cgaccgcagg catgctccag cgggagaaag tgaaaatggc
     4441 gtagggggca accaccggag ctctcagccg cgacacccat tccgcggcct cttcggggcc
     4501 ggctgtgtat tggaatgact gcgtcactaa ctcttgatcc aagtagcggc agaagccgtt
     4561 tgcgcctccg taccggtagc tgaacacgct tttcgaaatc ggcaggaaag caatctgcgg
     4621 ctggtagcgt tcccggattt cttccatcag atctaccgtc tctccaaaag tatcccgaaa
     4681 gcagtccacg ccgccgtata cggtcaggcc tttcgtgcgc agcacgtatg tgtagtggtc
     4741 gcactcggcg ccgggttcat cctgctcgcc ataaaacggc gtgggaatca cctcgatgtc
     4801 gtcgattggc gccggctccc acaaccgggc ctcccggata tcagtaaatc cgagcatttg
     4861 cagcgcgccc gctatcggag gattgaatgc ggaaggccgc tctacgcgcg ggatgatgac
     4921 cggagtgctt ttatcaaacc ccatcaatgt ttgaaaattg cagtgatccc aatgatgatg
     4981 gctacagcag atcgccccga ggcggatttg ttggacggct agagcagacc gggcgtacgc
     5041 tgcttccgtc gggagcagcg gatccatcaa cacggcgctg cgcccggact ggaacaaaag
     5101 ggaagtatgg cccatgaagg taacccgcgg cgcgggcctc cgcaagaatc cggcatcccg
     5161 gggttggcga tcgagcaact gatgggtttc cagcagagct aatagctctg cggcccacgc
     5221 cgcatcatcg gccggcaaat ttgattttaa aagcgctgcg atctcttcct gcggccggtt
     5281 cgatccgagc agcggccaca agcgtcctag cgctctcaga cgccgccgcg gcagggggtg
     5341 agagacagat tttcccgcgc ggtagaattt gagccgaagc tttcgatcgt cgaaagggcc
     5401 ctgctgccga aaaacagaat cgcggagaac ggccgagagc ctgccattcc ggcgaatttc
     5461 gtaatatttc gtgatttcct tcgaggactc aagccttgcc gccagcgagg agggatcgcc
     5521 cgattccgcg aaacaccggg ctaaattttc ataagcgtgc acgagcggct gtagatgttc
     5581 gcccaaaaaa agcagacggc aatcgaggtc gggagcataa agggggtcaa gctctccata
     5641 ctcgatcccg atctttactc ctggcgcgat accgctaatc agcatacagt cgttttgccg
     5701 gagggggatg gaccggctgt ctttcagatt tccccaacct tcttttcgat ggcgtccagc
     5761 aatgccggca gcccattcaa atctggctgg aaccgcagct caaaaaccgg caccttcgtg
     5821 atcaagttca tatagtgatg aaacatcgac ctcttgcggc ttttatcgag caggctgaaa
     5881 cagagagcat gcgtgagaac agcggcgaac gcccgcgccg aatgaagacg ctggatctca
     5941 cagatcccgc cattccgcgc agaggatgtg cggttcagga caaacaatgc tgccagcggc
     6001 ctcggctccg tttcgcagcg cacggatcgt tcgaggcgcg agctgttctc accaaaaaag
     6061 gctgccgagg gctgccgcag tcggattcgg aacggcagag ggattccggt gacgcggtca
     6121 cctgaggcgt cgaaagccac ggcgtcgtct gcccatagcg gatagccacg gcaactcagg
     6181 ccaaaagcga tggttgattt tcccgattca gagacgccgc aggttccgac aatcccctgg
     6241 ggcatcagca cagcgctcga atgcagagcc tcccggccac gcgcctgcag agccaggggc
     6301 aagatgctgc ggcggaagac ctcctcaatc gcccacctcc gcgcaggctg ccaggggatg
     6361 gccgtgaccg ttccttgatc tggtccatcc aggcaaaacc tcgccacgcc cggccaatca
     6421 atccacgggc ggccgtccaa gctgtagccg taagcgcaca gagcgccgtc attgtcgtgc
     6481 catgcttcca cgcccgggtc ggaccagtcc acctggggaa cagaatgcga gccttcggct
     6541 acaataacca gattcagcat cggatcgacg ccggatcaca aatcacggct gcggagggtg
     6601 cgtgtgcccg ccgggggcgc cctccgcttg tgattcgctc cagggtaagc ggcgaatcgc
     6661 taagtcccgc tttctttcgg gctaggcttc cggacttccc ctgggtcact gggagccggg
     6721 gtctttttac cgcctccacc gaggatcaca tcccgggcgt gaccttggta ggttacagtc
     6781 atcgtctccc aaggcttctt tgttccattt ttctcggcgc tcatagaatt cttctcctca
     6841 gagtctcttc ttcgcttagt caaagacgcg gccgaagcca cgcctcataa gtcagaacgt
     6901 gaatgatacg gtaagcctct ggcccgcagt tcttggccac aatctcgtca atttccgcgg
     6961 tcaaaatttg gggatcaacc aggcccagct tcgccaaagc ccgcgggccg cccagttcat
     7021 gccaggtctg tgcacctttg gtcaagatga cggaccagaa gaaggcggtc gcttccacct
     7081 ttttttgcct ctcaaaacct agatcaggga accggcgagc taaagactcc cgcaccaaac
     7141 ccttgctgcg gccacccttg ttcaacagca gcggcgggat tctatacaag agatcgacca
     7201 ggtcggaatc ccagaacggg tggagcatcc gcacccccat cgacctggcg ttttcgaaac
     7261 tctcctccag ctcccaagag accagagggt gatccagcgc gctgcgcatc tcttgtatat
     7321 aaaacccggc tttgtccgat tcgctctccg gcgccgcctg ctcgccccgg cagatcagtt
     7381 cccggcgtac agcagcatcg ggcgcgagcc acgcagggac gcgccgcgca cttcggctcc
     7441 ttcgggcccg cagcagcggc tccggcgcag cctgacgcag cgcctggatg agcagcggct
     7501 tcaggccgca tttccagagc agcagccgaa aaaccgccgc cggggagcgg cgaacagact
     7561 ggtgcataga acgccaaagc tgataaaagc ccaccacgtc cgcgcggcgc aacagatccg
     7621 cagcaagaaa cggggtgacg gtcaaccact cgtcgccgcc agtccccgta agaatagtcc
     7681 ggcaaccccg ctgccggccg gcttctccca gatggtgata ggcaggcaac caggtgttca
     7741 tcaggggcat ggagaagcgg ctgctcatct cgagagcggc ggatacaagg ccggctggcg
     7801 ccaccgcctc ctcaaacgga atcaccacct gctgcaaacc gaggccctcg gcgacgcttc
     7861 gctgcacgct ctcctcgttc gcctccgggt gtggaaaaac gagcgagaga gcgagcgggt
     7921 ctggcaagcc ctggtcccgg cacttctcgg cgcttacggc agccacggtg acggagtcca
     7981 gcccgccgct cagaaaaatg cccgccggcc caaactgcaa gcaccgggtc accgcttgat
     8041 ctagcaactg gtcgaactgg tccagttcgt cttcccgaag ccaattcacg cgaccattcg
     8101 gtgaagcggg atcccaatac cgataaactt gtttctgacc gtgagcgacg cgcaacacgt
     8161 ggcctggcgg cactcgcttc acgctctcgt aaacggtctc gtgcaaatcg gcaaagcgga
     8221 accgcaactg atcggcgatc gcagggcgat tgaaagcccg tgatacccgg ggatgccgca
     8281 gcagggcctc gatcgacgtc gaaagcagca gttcccgcgt ggtttcggcg tagaacaagg
     8341 gataaattcc gaccggatcg cgcgcgcaca gggagagatc gctttcgctg tcccacagca
     8401 gcaatgcgaa atggcctttc attttccgca gtgcatcctc gccccaccgc agatagctcc
     8461 taagcgcaag actcgcatcg ctctcggcct catcaagggt tcgctcgagc tcgcggccca
     8521 gttccgcgcg attgtagagc acgccgtcga aaatcacccc gcaggatcga gcctgtgcgg
     8581 cggagggctg agcctgttca ccggcggcca gcaaggacaa ggcgcgagag cgcccgtcgc
     8641 tcgtcacaat cgtccacccg gcgtgttgcg cctctgcacg cgggtcgccg ccgagcgacg
     8701 ccagccacac gccgggcagc tcgcgcctga tctcagggtc gccgaccagt ggagcgctag
     8761 tgggaaacaa cgacatggat caagtcttcc ttggccagcg aggccaacag ttgattgacc
     8821 tcgcgcgcca gctccgcggg gtccacatca aactcgcgca ccatctgttc gtggaccgcg
     8881 gcagggccgt tgccggccgc cagcaattcc cagaacctcg ctcccgtctg gttcagctcg
     8941 taaaatttgt cggtccccag atgcagcagt acgaccgtgt cgcccacgcg ctggaaaacg
     9001 acctccgcgt tcggttcggc gcgcaatggc agcgcgctga tgccgtcttc ggtgttccgg
     9061 gctgtcattc ttttggacct cgcgttcctc agttgtatgt tatctcgact ctcttgtcaa
     9121 tcacgaaacc cttgaaaaaa gcgattccga aactatgtat cacacatgta tacgaaccgt
     9181 accccagccg gctctccgct cagcggccct gcgccagcgc cggccttccc tccggcagca
     9241 gcgcgacggc gtccacccag gcggcgccgg cgatcaaatt gtcaaacttc atggaaggcc
     9301 ggcgcaccac ttgcagctcc gccaacccta tgtagccggg cacattgacc acggcctcca
     9361 cttttttcca atcactcgtg cccgtcacgc tgggagtgct tacgtcaagg cgcgcaggcg
     9421 attgcgggtc aacgatccgc aagccaatgc cttcgtcggt agtcaaatct tcagtccgca
     9481 cataagcctg caagcggtag cggcccggct tgagagggac cgcctcaaag acatgccggt
     9541 aatccacgtt ttctgtcccg tcgaagcgga tgcgcagcga tctcgcgccg gaatacgaca
     9601 ctgtgcggtc gagcgccgct tgcgcgccct tggccggctc aatgcgccag tcaaggcggc
     9661 atccggtgaa atcgcgttca aagtctccgt tgaacgcccg atcggattgt gtctgttggg
     9721 gcattccacc ttcaaactgg ctccgccaga cgcgcgcagc gctctcatag ttcctgcgct
     9781 tcaacaagac gtcggcgtat tcaccggcca aagccacatc cgtgaacgaa cgggcgctta
     9841 accaatccca ggccaattgc aggtcgcttt ctccgccatc tcgaagcaaa taccggaaat
     9901 acgattgggc ggcgcggcga ttgttcggga ttccttcccg cagaacttct ctggcggaac
     9961 cgacaaaccg ggcgtacgag gcaaagatga ccggatcgta atccggaaca atctccaata
    10021 ctcgagccaa gcgccggaga gcctcttgcg tctcgccgag ttccagatcg aggttgccgg
    10081 cgcgaagaag cacatacgga gagtgagggc cccgctcgat cgcgcgcgcc atgcaatagc
    10141 gtgcgctcgc ggcttctccc aactcctgaa atgcttccgc caagtcgcac caccggaaag
    10201 gggaggccgg gtccctgcgc aacgcctcgc ggaagccgag cgcagcggct tgcagcgctg
    10261 cgcgagtacc tccgagcagc gactggctgg caattggtat cagaaaatca ttcccgcaga
    10321 cgccaaatct gcacagcgct ggctcttggt ttggattggc tgcatatgcc cgatccgaca
    10381 agatacgacc cgctgcatag gctgtaagga aaaaacttaa cgcgaacaca gacaggcgac
    10441 gcaaaggatc caaccgcaat gaccaataaa aaatggccaa caaagattct tgcccggata
    10501 gagtattcga ctttggttct ctttacaagt accggtactt tttgagcata gtttgggtgc
    10561 tgcggcgctg tcgcggaagt tcgctaagcc tttcagggtc attgtattac tgctcatgcc
    10621 gaaaacaggc ggatttggcc ggcgtccttg cgcggcgcgg aagttttgat gcttgacata
    10681 cgtatagttc tgccctataa ctgcgaatac cccagcaaaa aagctcaacg taagtcctct
    10741 tttcctggga ctcagatcgc ggtagcgcgt ctgcggctga gcggcatccc atggtgaata
    10801 tgcattcgtc ggagactgtt ccagatcgga attggtttgc tttgcaggtg aagcccaggc
    10861 atgaaaaggc cgcagcgaac gcgctgcggg gcaagggatt cgaagagttt cttcccatgt
    10921 gcacatccgt gaggcgctgg tccgatcgat ggaaagagct tcagacgcct ttgtttcccc
    10981 gttatgtgtt ttgccgcttc catctgaaa
//
LOCUS       DASREK010000278        42710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_300449, whole genome shotgun sequence.
ACCESSION   DASREK010000278 DASREK010000000
VERSION     DASREK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 42710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42710
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300449"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFA54_15025"
     CDS             <1..403
                     /locus_tag="VFA54_15025"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HZS52178.1"
                     /translation="GVARHHVLRCLEANQPYVPIARQGSPSGQESSAREPAAPGDILG
                     ELTRHERIEATRRAVLSLPPNYREVVVLCDLQELSYAEAAAALGCAIGTVRSRLHRAR
                     ALVAERVTSLLKDSVRSRPASPTAKAARCFV"
     gene            400..1131
                     /locus_tag="VFA54_15030"
     CDS             400..1131
                     /locus_tag="VFA54_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52179.1"
                     /translation="MNCQDFEAIVNDLARDQLILVADRARALVHTETCARCAARLADE
                     RMLTAELRALAAGSESQEAPAPVEEALLRAFRRQKAASGAVAAKGRAWVGAVAVGALA
                     AAMLILLALTIPALRRNGPPEPKPAEGLRETKPPRKQRIEVAEAPVIDDSMLAGDETA
                     AAQLLPIDLTAGLLNSALDVFVPLHYGETLSDLDGDRVVRVELPYSALISLGVSMDED
                     AGNDGIEADLLLGDDGLAHAIRLIR"
     gene            1165..1989
                     /locus_tag="VFA54_15035"
     CDS             1165..1989
                     /locus_tag="VFA54_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52180.1"
                     /translation="MKARPKIIHNLTRFFLLLGVGAAVFAQGPPRRQGPDMGFPPGPG
                     GGSVIRFVGQVVKGAPFSGTGVTTFSETLSNGNTITRANCVKIYRDADGRTRREETPN
                     SSACSATPRFIVITDPVAGTESILNPANNTYRQFKLRTPPNAGANRPPHAPGGNAANV
                     QTGPATPPTITVPGTALVANGTQTTITIPAGQIGNAQPIVITSTRYFSSDLQVVVSST
                     RMDPLRGNTSYTLTNVSTNAPDPSLFTIPAGYTLQQGPGGRGLGKAQQEWFHRDGR"
     gene            complement(2119..2595)
                     /locus_tag="VFA54_15040"
     CDS             complement(2119..2595)
                     /locus_tag="VFA54_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZS52181.1"
                     /translation="MPVLERIENFLDDLRVPFARSAHAPAFTAREVAQAENVSSRKIA
                     KTVVLVVDGAFVMAVLPADSIVDIQELRRALGATHLRLATEMEIGQLFPDCELGAMPP
                     FGNLYGLPVYAEVSLAQQHTIAFNAGTHRDVVYMNFADFRRAVNPVMLPFARRVAA"
     gene            2898..3323
                     /locus_tag="VFA54_15045"
     CDS             2898..3323
                     /locus_tag="VFA54_15045"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZS52182.1"
                     /translation="MKRTASAIWQGDLKSGKGIISTASGVLSNTQYSFSARFEQGTGT
                     NPEELIAAAHAGCFAMALSAQLGEAGLVAQRIDASATVTLEKTDGGFTVTSSHLQVTV
                     KVPGGDRAKIETAANNAKAGCPISKLLNAKITMDAKYET"
     gene            complement(3566..4321)
                     /locus_tag="VFA54_15050"
     CDS             complement(3566..4321)
                     /locus_tag="VFA54_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52183.1"
                     /translation="MFRDSRPVRFASLCGWLTAIVFFIVGLVETLRFTRWNPYAKTDW
                     SAMLRSLQPRAGGDFVLHSFIFCVCLAGLFFLLALGSRIYQTHPTTTVTGLGFLGVPL
                     VLMGIYNVWLAFGHDMLVLQYQRTQETGLQYLYQAGLFGLPYMAALVAYFGFWGFVFL
                     GYAFGAQRRALGLRGWCWASAAGLIWSIVVSFYGYHQIYAANFFPRTLMIFGDLGLWI
                     LPTITLAISASWLWTQSRLAMVQPVRPEVSKAA"
     gene            complement(4570..6480)
                     /locus_tag="VFA54_15055"
     CDS             complement(4570..6480)
                     /locus_tag="VFA54_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52184.1"
                     /translation="MEARLAVVLAAVVLIRLPFLQQPIQGDDVYYLAIARNARVDPLH
                     PMQMGYTFQGARVSMAGHPHPPLNAYILAGLIHLFGGVRELPFHTVYLTFSLIAAAAM
                     YRLARRFTAHPLLAALLFAAVPAFVVNGNSLEADLPFLAFWMAGFALYFEGNQKLAAG
                     SLAVAALAAYQAVFAAPILAHHAWYRRRRSTGAWVAASAAPATLACWQVFQRMTSGRL
                     PAGILAGYLSSYGFLGFEQKARSASALVVHLGWIVFPPAALWAPLWAWLPAPLAVAAA
                     LAVKGYLWWQRALLAASLVAGSAILTRWIRDLWRRRSSEDGILAAWGLVYFAGAVVVF
                     YAGAARYLLPLAAPVVLTLVRVLPRPRLLWAAAAANLSLGLTLAASNYQYSERYRELA
                     RELQKSATGRPLWSGAEWGLRYYLEQSGAEPLDKEQPVYPGSVVVESELAGKIPFTSS
                     GGRLRRISQAEIRSSLPVQTIGLDARAGYSSSGFGLLPFDLGRGVLDRISVSVLEKAE
                     PQLSYLRMNDPAAAPQLLSGFYDVEDRAWRWMEGQAVAALKPAETGASVFTISFFIPD
                     AAPARRITVAVDGIEVADRAYAAPGRYLLSAPVKLPPGPACEVIISVDKTFQAPGDQR
                     RLGIVVQEFGLR"
     gene            complement(6465..7943)
                     /gene="gcvPB"
                     /locus_tag="VFA54_15060"
     CDS             complement(6465..7943)
                     /gene="gcvPB"
                     /locus_tag="VFA54_15060"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HZS52185.1"
                     /translation="MIRKVRTHINQNEPLLFERSSPGKKAYQLPPLDVPPVDPAAALG
                     AENVRREIAGFPEVSEVEIIRHFTRLSTWNYAIDLGVYPLGSCTMKYNPRVNEHVARI
                     EGLAQAHPYQPEQLAQGAMRIQVCLEELLAEITGMDAVTLQPAAGAHGELTGILLARA
                     LLESRGNARKKVLIPDSAHGTNPATAAIAGYQVESIASNAEGMVDLAELERHLDGDVA
                     ALMVTNPNTLGIFERDIARVSGLLHSRGALLYMDGANMNALAGLARPGDFGIDVMHLN
                     LHKTFSTPHGGGGPGGGPVAVKREFEPFLPVPRIARDNGRLHYDYDRPQSIGRVRAFY
                     GNFGVLVRALAYILAHGGEGLRNATLDAVLNANYVRKHLEGCYDLPYPSPSLHECVFS
                     DARQAARGVRTGDIAKRLIDYGFHPYTVSFPLIVQGALMIEPTETEDKRELDLLIDAL
                     RSIAAEARQDPELVRTAPHNTRVKRVDEVAAARKPVVRWKPG"
     gene            complement(8034..9359)
                     /gene="gcvPA"
                     /locus_tag="VFA54_15065"
     CDS             complement(8034..9359)
                     /gene="gcvPA"
                     /locus_tag="VFA54_15065"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HZS52186.1"
                     /translation="MRYLPKSDSERREMLASMGLDSIEDLFSHLPEELRLKRPLQIPP
                     GKSEYEIIEYFRERAAENANGYSSFLGAGAYYHFRPVIIDSLISRGEFLTAYTPYQPE
                     ISQGTLASIFEFQTMICQLTGMDVANASMYDGSTALPEAVMMACRITGRDRAVVARSV
                     HPEYREVHRTYARHQGLPVDEAPYEKQTGSVDLGRLAALVNEQTAAVVVQSPNFFGVV
                     EPVQKAARIAHEKGALLIVMFTEAVSLGLLQPPADADIVAGELQSFAISPSYGGPYVG
                     ILAAKEKYIRQMPGRLVGQTVDKEGNRAFCLTLATREQHIRREKATSNICTNQALIAL
                     MATIFMSVYGKQGLRELAEQNLAKARYLAGALEARGSKPRFSGPFFNEFVVQCGERNP
                     ADINRRLLERKIIGGLEMGRFYPELSDCMLLCATETTKRVEIDRLAEHF"
     gene            complement(9403..9792)
                     /gene="gcvH"
                     /locus_tag="VFA54_15070"
     CDS             complement(9403..9792)
                     /gene="gcvH"
                     /locus_tag="VFA54_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HZS52187.1"
                     /translation="MYPEDFRYTKEHEWIKVEGDTGVVGITDYAQKELGDIVYVDLPK
                     VGAKLVAGKTMGSVESVKAVSDIYSPASGEVIEVNNELAEKPESLNEDPHGSAWLVKI
                     RLSDRAELDGLLTAAAYQEYISAETGH"
     gene            complement(9799..10911)
                     /gene="gcvT"
                     /locus_tag="VFA54_15075"
     CDS             complement(9799..10911)
                     /gene="gcvT"
                     /locus_tag="VFA54_15075"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZS52188.1"
                     /translation="MEIAGATTALQRTPLYPTHRALGAKMVDFGGWEMPVEYSGIISE
                     HMAVRTGVGVFDVSHMGEIEIRGPQALDLVEWVTPNAASQLHDNQAQYSALLYPTGGF
                     VDDVLVHRISAQHYFLCVNASNQKKDYEWIRAQNRFDAAVECASERYAQIAIQGPMAL
                     ATLQKLTRTDLAGIRYYWFSRGEILGTPAIIARTGYTGEDGFEIYVPPDFADRAWNAL
                     LEAGREFAIRPCGLGARNTLRLEARMALYGHEIDASTTPYEAGLSWVVKLDKGDFIGR
                     DALARQKAKGPARRLAGFEMRCRSIAREGYLAMIDGAPAGFVTSGSPAPFLKKNIGLC
                     YLPAGWAAVGQRLEIEIRSQPAPAEIVPTPFYSRKR"
     gene            11294..12442
                     /locus_tag="VFA54_15080"
     CDS             11294..12442
                     /locus_tag="VFA54_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52189.1"
                     /translation="MIRRLALLVAALLFADSLLYLALRILDSSRIFYDRSPVDARRLH
                     YWVQNSYDPELGWDVGKGSKNNVGSARRGDYPAAEQYKLKAFGDSFTFGSDVGDGQTW
                     EAAIERETGWACLNYGVPGYGPDQALLKYQRTGVHTQFTILGVQEENIARVVNVYRAF
                     YMEDWGPPKPRFFLEAGGLRLEPNPIARTEDASRLLDPAFVDSLRRLDYWPRYNEDVL
                     GAPRRLRWPATWIVLSHAPFFARRAVLEVRLRWSPGYEDEARRFKPYHLYAESSEAFQ
                     ILVRIIDRFAELCAARGERPLVLVFPMEHTVDLMKTYGKCIYEPLTRRLDASSIPHID
                     FGPIFAREDYRQYYVRYNGHFSAAGNERVAREIIRFIESPGAGHSGSR"
     gene            complement(12557..14254)
                     /locus_tag="VFA54_15085"
     CDS             complement(12557..14254)
                     /locus_tag="VFA54_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52190.1"
                     /translation="MAAERQAPHWIPRLSFFLLALVYLFYWQDVEYVADDWLHLNFCL
                     QAAGTGLRGHLHILRALLENRLYGGFQLFWVSQWLNCLLVWTFGYAPKIAYAAVLLLH
                     AGNACLFYALLRRLRAGTRMAYLAAALVVLTPVAHGPLFWPVNCTFFVLPMLWLFLYL
                     LQALKTYETGKLTVRAAVLQSGLILLVLFSGSPASALLLASPLWLGICFFGRARLRTG
                     LLVTLLNLAVVVPAVGGYRWYLQSLRRPGGHEERFALTYEYGAAGLRMTRTHLINLSG
                     LTPHSYYRLSGISSAALLAGLACGLLVLAVARRLPSADRQQSAGSLILFAAGMWVFAY
                     APIAFLNSKTLRHYYPFSFFLCLLLALPAAWSAAIRARLLALGWAALLCGYFAVCTAA
                     EIQQCWVPESLATQSLKPALRELDHLSPGDWIIAPGMPIVIGTAPHFSLALEPGGQNF
                     ARYWTGVSGLRFAREIVVERGRLRFFHQAFMRDTSAEELAARVHVIARNESGVYTVRR
                     YVAIERRPGLYEVLPLKGFAALSGSGGLASQAQLAPLETEIYYAKPFVHGDIRYLHY"
     gene            complement(14630..17974)
                     /locus_tag="VFA54_15090"
     CDS             complement(14630..17974)
                     /locus_tag="VFA54_15090"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS52191.1"
                     /translation="MTIKRCGLTGVFLLLAAGALPAQEATGTISGVITDSTGAAIPGA
                     AVEVQNRQTNFTRRTETDARGEYNVPALQPGLYNVSVTREGFKKAVYSNIRLEVNQVA
                     TVNGTLEIGTVTESVTVEGAATLVQSETTTVGQVVETRKIEELPLNGRQFLQLTTLVP
                     GTISGYTRDASRQGGRRSLLNIAVSGGRTEFNNYVLDGVLNTDENFGTYIVSPSVDAL
                     QEFKVQTSSYSAQYGRGGAGQINIITKSGTNQLHGTAFEFLRNSDFDAKNFFDRSDKP
                     IPPFKQNQFGATAGAPVYLPKIYNGRDKTFFFFDYEGFRIRQALTQVSTVATPDQRAG
                     DFSKSGVTIYDPRTLQPDPSKAGGFVRSPFPNNAVPQNRLDPTSQYLLQFLPTPNLPG
                     TSNNYLSNEGLSTNDDQFTGRLDHKMGPNDNIFGRYTLSDESTFNPGVFPGIGTFVNV
                     RGQLLAVGETHVFGPRTVNEFRFGFNRLSNALLQQNAFKLDAVGQAGIVGLARNPIDF
                     GIPAVAVQGITSWGDQTFGYPSLLRDNVYQYIDNLSLTRGRHNLNVGVEIRRFQYANF
                     ADNLPRGSYTFASPLFTSNPASPSGTGAGMADFLLGVPHSAEGSVGDTSIYDFRNSWN
                     VYVQDDVRVSRKLTVNLGLRYEYNPYPYEKYDRIETLDLTTNPPTIVRAGNGDPYFFY
                     PKNVNLNGIPYVRDGRLGRSLLQADKKNFAPRVGFAYSLNPKTVIRSAYGIFYTQDIG
                     NPFFDLARNVPRNVRAALTSDPNTPQLDMKNLFSGLAGNQSVLVPTLTMTDPHYPTGY
                     MQQWTFNVQREISPDLVVEVGYVANKGTHLGILNLLNTAPPGPGAAQPRRPYPIYERI
                     FFIQHILNSTYESLQAKVEKKYSRGLSFLGSYTFGKSLDYASSTRTSGETNRPIDPTN
                     LKIDHGRSLFDARQNFVFNTLYELPFGPGKPFANSGGLRGRLVGGWQISGIFQARTGL
                     PFTVSASGDPANTGNDSSGRAQLLPGKNPVLPASQRTPDQWFNTAAYAPPAPYTFGNA
                     GRNTLDGPGLVNFDVSMIKNISITEGRMVQFRSEFFNLMNTPHFGLNSQGSVQAPNRS
                     VNGSGFGKISAAGPARQIQLALKFIF"
     gene            18336..20480
                     /locus_tag="VFA54_15095"
     CDS             18336..20480
                     /locus_tag="VFA54_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZS52192.1"
                     /translation="MNIRTVFDRSLRACLACAACFLAFGAQGPAQTRRERFDVVIISG
                     SSAGVGAALGAGRMGASVALIEDTPVLGGMLANGISNIDAYSLESLSGVFDEFRLRVQ
                     EHYRPIMASDPIFRTRMRMPRHVDGRSRAANDAEAGGRWEPHVADEIFKDMVAKVPKI
                     TVFYNRWPTGVIKQGNRVIAVNTDDARGDRKTFFGKVVIDATHEGDIAAWAGAAYRVG
                     REARSPLEPHAGDIYFFDGTGEIMDGGGQQDRAVASYGLRLTIQHYSDRRDSSHILQT
                     PPPGYDKSKYEHSAYDAAITMPHGKAEMNANPIGNEIQEINWEWPEAAREQRRRLYEV
                     YKNRALGFLYYLQHERGRTDLGLPKDEFTDNGNVPYRVFVREARRIVGVKTMTEADIN
                     PFLNGRGWIPPVREDAIAIGHYPIDSKPVRSKTDFSRPDKGAGDFYIVNVTTPFQAPF
                     GAIVPEKIDGLLVPAALSATHVAFSSIRMDPTWTVLGQAAGVAAALSARLNRNVRSVP
                     VEQIQRELLKQKCKLMFYWDLPADHPAFAAVEWLSVRNAVAGYPDRLFRPDQSLTRAE
                     MAALLVKAFQLWPSVSDVHFTDVAPTHWAFREIETLFDNRALAAFGISPRWPEAGAYD
                     PARDEGFAASKRSGEFLPARPVLWKELAETLWAIRKPHDEGEDPSSWIRSVLRGSMFG
                     APYAGRNFPAGAVVTRGEACALVASLSDNGAN"
     gene            20609..21763
                     /locus_tag="VFA54_15100"
     CDS             20609..21763
                     /locus_tag="VFA54_15100"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZS52193.1"
                     /translation="MSVLWSRLLGYARRLGAPLVLLYAGSSLAAQSAAPSAYIGARAC
                     AECHADRVSRQQSAGHAQALAPAADHPLAAAFTPPTMFTRPPNFRFQFLRPKQLQVRV
                     FDGDNTIELPVEWAFGAGAQAVTFVTRVNEQWYLEHYFSYYSGLRSFAPTPGQDAIRP
                     SSLVSAVGLLYKALDPDTGIVGCFECHSTGPVITGSEGIRPRELGVRCEACHGPGSAH
                     VRAAKEGPSNEIRKAIQNPGRLPASQLNVFCGRCHRPPAAKGTAIDWNYAWNVRHQPV
                     YFSQSACFRKSQGALTCLTCHNPHEPLRRDNAFYNQHCLDCHKHSGGEPQIRAAVKAH
                     PPRPVCESRQPANCIDCHMPRVSPQAGLRFTNHWIGVYAEGAKLKPRSEQ"
     gene            complement(21836..23068)
                     /locus_tag="VFA54_15105"
     CDS             complement(21836..23068)
                     /locus_tag="VFA54_15105"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HZS52194.1"
                     /translation="MKKLSNQNQRRPAMQAGAAALFLFLSLPALTAQVKTGLDVLVEE
                     DFAPLAGKRIGIITNHSGLTSDGRRNIDVLRRSGKVKVAVIFTPEHGLTGLRDDPNIP
                     SSIDPTTDIPIRSLFDGATRKMTADMLKDIDALVYDIQDIGARFYTFITTLGYGLEAA
                     AQAHIPYFVLDRPNPINGIAVEGPRLDEKYVSFVAYMPGLPIRHGMTVGELARLYNGE
                     KKLGADLHVIEMKGWNRRMWFDETGLEWVNPSPAIRNLTEAILYPGTCLLESRQVSVG
                     RGTDTPFQMIGAPWFKAREAAAYLNSLKLPGVSFIPRRFRPNAYLFKDEDCEGVDVQL
                     LNRDTLDSVRMGLELVHATLQFHPGLFNVDGIMRLLGNEDAGARLKRGETGSEVNRSF
                     QKDLAEFRKVRQQYLLYP"
     gene            complement(23137..24069)
                     /locus_tag="VFA54_15110"
     CDS             complement(23137..24069)
                     /locus_tag="VFA54_15110"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZS52195.1"
                     /translation="MTLFLGVDGGQSSTRAVIGDESGRILATGEGGPVEHIGAPQGRE
                     RVFRALQESAGAACRQLGRDLADTSFEAAFFGFTGADAGKRAIVEELIHARQIALSDD
                     SVIALTGALGGEPGVVTIAGTGSISFGRNQAGETARAGGWGYLFGDEGSAFDLARQAL
                     RAALRFEEGWGPSTALHRMLCEAVGLNDIRLVQRKLYSAEFPRKRIAALALLVDEAAR
                     LQDPVACSILENAARDLAAITNVVRTRLFRREESVAIAYVGGVFRCGMLRSEFIRLLS
                     SCGTNRLIAPLHDPAVGALIEAYRLAGARVLVSQ"
     gene            complement(24066..25283)
                     /locus_tag="VFA54_15115"
     CDS             complement(24066..25283)
                     /locus_tag="VFA54_15115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS52196.1"
                     /translation="MSYRWELLLWLWLAFFFNQADRQVFSVVLPSIRSDLGLSYLQLG
                     LVGSVFTAALAVMVPVAGFLGDICSRKAIVVVSLLAWSLATLCTGFGATLLYLVAVRG
                     TATAVGEAFFAPSAFAMLSAEHTETRARALSLFQTAVYTGLIASGWMGGAIASRWGWR
                     AAFWVFGAIGILLSLVSFFRLRDSRSPAPERRAAVRETVAAILSTPTARFAALGSGAM
                     IFVNVGYLAWMPTYIYERFGVSLAAAGLASMLFHHLFAFLGVMIGGILSDHWAPRRPQ
                     VRLEIQGLALLAGSPFLYFLGLAPSEGLLYAALAGFGFFRGLFDSNTYPAFYSVIAPR
                     YHASASGLLIAFAFLIASLAPVLLARAKMTVGLAGGFSALSLVYLAGAALVMWGAYRH
                     FLADWKVVNGAAV"
     gene            complement(25351..26073)
                     /locus_tag="VFA54_15120"
     CDS             complement(25351..26073)
                     /locus_tag="VFA54_15120"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF002231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HZS52197.1"
                     /translation="MREDLPPICRHFYRRLVVSCQAREGEPFRDPNCMARFAVSAVAG
                     GAAAIRANGPHDIREIRKAVSVPILGIWKQPQEDGRPLITPSGEAAQALIAAGADMVA
                     IDVTCRGQRYGALGRLARIRRDLNVPALADIATLEEALAAAQSGANFVLTTMRGYTEE
                     TAHVRSFDLAFLRELVRRCPVPVIAEGMIETPEQARAALDAGAYAVVVGSAITRPVTI
                     ARRFAEAVSQAGAGRSEPEPAS"
     gene            complement(26293..29733)
                     /locus_tag="VFA54_15125"
     CDS             complement(26293..29733)
                     /locus_tag="VFA54_15125"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS52198.1"
                     /translation="MSANSVLRGVLLALLAATLSFAQQITGSITGTVMDPAGAAVANA
                     QVKLTNTGTGAVENAITDESGNFRFLLLPPGTYSLEASAPGFKLFRRDGIIVEVDRSL
                     AVPVALQIGQVTETVEVQGGTPLLEPNTSSLGTVMEEKKVEDLPLNGRNPMGLANLIP
                     TVRGIGYFGGQVLSSWRLAAVGIGGGQPLTNGYLVDGIANDKMVDSGPMTFLTVDDTE
                     EFKVQTNSMSAEFGRTTGGVISMISKSGTNELHGSLFEFLRNDKLNANDFFANKAGRK
                     IAPDKVNQFGGTLGGPIIKNKLFYFFNYEGYRERSASIETITSPTAAQRAGDFSGLTT
                     STGQLITIYDPTTTVPDPSNPGRFIRSAFPGNKIPADRINLVGQNILSYYPQPNLPGL
                     SSNLFLTGATPINKDTETIRLDYNISPTKRLAGRFTDDRLAWQFANFFNNIADVDGRL
                     ISIPRRNAFISYTDSFSPTLLFDSRIGFSHQTEAYSIPSQGFDITKLGLPASLLAQSQ
                     NAPNAPQGVFPRLSVSDLTTFGGTAAAANHTNTGSASATLTKIRGSQTWKFGYEFRLY
                     QRNEFTLNSPVGSYTFNRGFTQGPNPDQASATAGYSVASLLLGTPASAFAGINASSAV
                     TLRYNALFFQDDWKVSRKLTLNLGLRWDKDGAPTERYNRYENFDPDVASPLKAPGLTL
                     RGGLVYSGVNGHPRAFYTSPSKDFQPRFGFAYQLQAKTVLRGGFGIMFVPTTQGQYTP
                     SQIGFSSTTPMVTSNDGGRTPANTLSNPFPNGLTPPTGASLGALTGIGTGVTGQLYNQ
                     GRGYSEQWNFTIQHQPWENWLVEVGYMGNRGVHLFMYNQDIDWLPNQALSLGSALGQT
                     VPNPFYGVITSGPLAAAQVPQYWLMLPFPQYAYTSLPTSQGGVISPISYLNNSIYHAL
                     TVKVEKRFSRGFSLLASYAASKLLDLGDNLTQVRPGGVSGYLVQNWNNLSAERSKSLY
                     DVPQRLVVTALWDVPLGKSGNPLYRAVAGGWQLNAITTLQSGLPIPLGANLTGVGNRP
                     SVVAGVSDKASSQSLSQWFNTAAFTAPAPYSYGNASRTLPDVLSDGLFSMDFSAFKNF
                     SVREKYQFQFRAEAFNLTNTPTFEVPGTMYGSPTFGQVTATAFTPKPRVIQFGLKLRF
                     "
     gene            30220..33690
                     /locus_tag="VFA54_15130"
     CDS             30220..33690
                     /locus_tag="VFA54_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS52199.1"
                     /translation="MFRSHRRGFLSSLSALFLLAALIAFNAAAFGQLITGTVTGSVQD
                     ATGAAIIGATVRLTNTGTGVVQTTQSDNSGNFQFLLLPPGTYTVEATSAGFRAFRREG
                     IIVEADRSLAVPVVLSVGQVTETVEVVGGTPLLEPNTSEVGTTVDSQKVIELPLNARN
                     PMGLANLIPTVKGVGYFGQQILTSWRIGAINIGGGQPLTSAFLLDGVPNDKLGDAAGA
                     NTYLTTDSTQEFKVITNSMSAEYGRTTGGVISVVSKSGTNEYHGGLFMYVQNTALNAN
                     DFFANASGTPLAPVHQYQFGGSLGGRIIKDKLFFFGNFEGFVQHLSASEVINSPTALQ
                     RAGDFSQTFAANGQLITIYDPLTTAPNPNKPGASIRTPFPGNVIPPNRISQFAKGFFA
                     LYPLPNLPGQKVTGTQNLFLIGNIPTDRETGGVKVDYMLNQNRRLAVRYTRDVLNESV
                     PNGSFFQNVLDNDKKIIYVPRHSGSVSYTDSLSPTLLLDLRSGINRDYDQSIPWSYVG
                     PYEKTGFPLTSLGLPQSLVNQLQPGAVQFPALTISDLGGILGGYGSAIGNRAAYTWGN
                     VASITKIMGAHTIKTGYQLTVYRGFPLDRTPLNFTFTRGFTQGPDPTVASASAGYGTA
                     SLELGTPSAGTATYQPSHEQQEIDHGLYVQDDWKATQKLTLNLGLRWEYQGPFTDRYN
                     EMTNFDPNVTSPLQVPGLTLKGGTIFPGVNGVPRGVVDVSYKHFSPRVGFAYQTMNKL
                     VARGAYGVFFVPEKGILNPASTGFGIVTTMVASLDNGLTPYNTINDPFPDGLLFPSGS
                     SQGLLTGIGTSISGQLRHVLPGYAQQWNFSLQYSPWNNWLIEGAYLGNKGTHLETLQG
                     LNLNQINPQYLSLGNALNQQAPNPFYGIITSGPLSAPTISRQQLLLPYPQYTAVNGGW
                     SYNGNSIYHAFALKVEKRFSQGFSILASYVASKMIDGAVGSGGAVRTGGTPETGIVNW
                     YNLRAERSKSIYDVPQRAVITVLWQEPFFKGSHGLTRQVLGGWNANGIVTIQSGQSIA
                     LQSGSASQRPNVVSGVDDHAAQQSLTQWFNKAAFSIPAPFTYGNASRTIPNIMSDGMF
                     DMDFALYKDFALSERFKLQLKGEAFNLTNTPTFDVPNRDVTSQSFGVVSATALNPRPR
                     SVQLSLRLAF"
     gene            33878..36394
                     /locus_tag="VFA54_15135"
     CDS             33878..36394
                     /locus_tag="VFA54_15135"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HZS52200.1"
                     /translation="MAKNCYLCHNEKLNTANLNLEAYRDAGLALQQTQVWQKVLEKLS
                     AGKMPPPGLPRPDPGQVNTVTAWISGLLHGTETPAKVNPGRVTARRLNRVEYTNTIRD
                     LLGVPFQAAGKFPTDDSGYGFDNIGDVLSLSPLLMEKYLSAAKQISREAVYGPSLPPK
                     PTLLVRLMAKKAQDTGGSLSGENILPYSMRGALYGTYWFPVDAEYEFRVRVANYRGQD
                     PNDLPPERRRRLEEIRRGLPVGQQQDQEQAQPPAGPAAAPKASRFRRPLTPEEIQARE
                     EESRKAFPPVTMVMTLDGKPVLTGVVEGDVNYQYSRGELVGRTHVKAGAHFLRASYPE
                     LADLPDPRKNVNPDGRRKLYVDYVNIIGPFNPSAEPPESYRRIFVCGHAPGRHRPECA
                     RKVLQNLATKAYRCPATSSEVEGLLRFVTLAQKQGDSFEEGIRVALEAILVSPHFLFR
                     IERDANPSDAASPHPVSQYELASRLSYFLWASMPDDELLRLAREEKLRDPQVLESQIR
                     RMLADPKSESLVDDFASQWLQLRNLDRVRPDSKRFSTVDDELLDAMRRETKLFVDAII
                     HEDRSVLDFIDAPFTFLNGPLARHYGVPGVTGEEFRRVNLSGEMAEQRGGVLSQGSVL
                     TISSYATRTSPVLRGRWVLDNLLGTPPPPPPPNVPQLNEAALGSTASMREQLEAHRKN
                     ATCAVCHNQMDPIGFGLENYDATGAWRTEDGKFPIDSSGVMPGGKSFQGPKQLKQILL
                     GESDLFVRNLTEKMLTYALGRGLEPYDEPVVSQISRRLAADNYRFSTLIAEIIKSTPF
                     QMRSGESGARGRPSRTDQQRVAGDYSKTRISGPQERKDEP"
     gene            36384..37745
                     /locus_tag="VFA54_15140"
     CDS             36384..37745
                     /locus_tag="VFA54_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HZS52201.1"
                     /translation="MNLSKKLARRTFLRGLGTSIALPFLEAMVPAWAATSALKSPCRM
                     AFLYVPNGIIMEAWTPQTEGPVAELPRTLPRVLEPLAPYRDNLLLLSGLSLKNGDALG
                     DGPGDHARAGASYLTAAHPKKTFGADIQVGVSVDQIAAQKIAGQTQFASLELSCEDGL
                     LGGNCDNGYSCAYSNSISWRTPSTPMPAEISPRALFERLFGGADASETPQRRARQRLY
                     EKSILDFVLEDTQRLKGTLGPTDRRKMDEYLYAVRDIEKRIERAERNNRQALPTVVQP
                     PSGIPESFKEHSELMFDLLAVAFQADLTRIATLMLGLEQSTRSYPEIGISEAHHGLTH
                     HLGNKEKIEKVTQINCFHARQFAYFLGKLNSIKDGDGTLLDHSMIVYGSGLSDGNRHL
                     HHNLPVLMAGRGCGTVRPGRHVIYPQDTPMANLYVAMLDRMGVQIESMADSNGELGYL
                     EGI"
     gene            38272..41538
                     /locus_tag="VFA54_15145"
     CDS             38272..41538
                     /locus_tag="VFA54_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS52202.1"
                     /translation="MALRGQQRRPCGRTRSGGQRWGSYKASGGQTLKATILGTITDSS
                     HAVIPSVQVSLTETTTNFHRTETTNDSGFYAFTNLDPGNYRVEVEHPGFRKTVRAGID
                     LVPNTTVRVDLELQPGAVTETVDVTAEAPLLQTDRADTGGKVESIQLTSLPMLWNRNY
                     QNLLQVVPGVGRSYRSNSQFFDSQDHLQSVVNGLDQRNNYMIEGVDNNVENLTGIIVP
                     ADAIAVVDTTTSNYDPELGRAGGAVVNVIMKSGTNDFHGSAFEYHYDNDMQAKNVFAS
                     TTPHAVYNQFGGSLGGRIKRDKLFFFGDYQGSRDRKGNTNLPTIPTMAFRAGDLSGSP
                     TTIYDPLTGSASGAGRTPFPNNQIPTARISSITQQYLSFLPAPTRPGFSANFEKPTST
                     AKAIDQFDVKIDYVITANDRMFVRYNFQRALVDDPGLYGPGGIYGGPHNSGFQGTGPS
                     RNQAPGINYSHIFSPTLVTEARFGIVRNRNDATNPDTGLPLSQKLGIPNANLGDMWTS
                     GLAEVYVDGYDTPMIGVNGCLPWRRSVTNFDWVNNWTKTKGNHVIKWGADIRRERQDL
                     LQTPTFSPRGRFTFTAGPTANPSDPKTSFGNSFAAFLLDQPNGVGRDLAFIFPARRNT
                     IYNLYFQDKWQVTQKLTLDLGLRWEFWPSSTPHFPAGFSNYNPFNNTLELAGIGSIPN
                     NLGIQNQPKSFAPRFGAAYRLNDKTVFRGGYGISYLPRTTNVYNFPVNQNQQLTAPNS
                     FSAAGSMAQGFPAPIAVAIPSTGVITNPPNSTFTYVAKDRYQTYVESWNVAIQRSLPR
                     NFAVDLAYVGNHTVNNGITWNINAGRVPGAGPAGQPLNQLFGRTAETDYSLPTHAFYD
                     AFQLKINRRFSGGLLLTTAYAYGKAIDIQSNFNNINPLSQKGPTSFDRRHVFTQSFLY
                     DLPFGPGKRWAHSGLAKWIIGGWQVNGLVTAESGFPFSITFSSATLNAPGNSNRPNVS
                     GPVAVLGNIGPGQLFFDTSKFSAPAPGTFGNTPRNFLYGPKLFSIDSSLVRKFRITER
                     ITGELQGQAINLTNTPHFDNPGASFGSSSFGQVTTAQGNQTVKVNENRQLEVSLRFVF
                     "
     gene            complement(41695..42501)
                     /gene="mutM"
                     /locus_tag="VFA54_15150"
     CDS             complement(41695..42501)
                     /gene="mutM"
                     /locus_tag="VFA54_15150"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZS52203.1"
                     /translation="MPELPEVEAVRRIAERALRGRRIVSAHISRRRLCAPQTPEEFTR
                     AVAGRRFVSSERRGKNILLHFDGISLWIHLRMTGSLYPVPGRQLPPASASAHFLLDNG
                     RALVFDDPRGLGVMRAAKRDEIENRLSGIGVDPLSKSFTADVLAALARSSRGAVKPLL
                     MDQKRIAGLGNIYAAEALFQAGIDPRRKAASLSSSRIGRLRDAIAQALAEAVRSAERA
                     YRKPGGFAEGESFHPAVYGREGESCRRCGRTVRRIRQSGRSTYFCPGCQR"
     gene            complement(42510..>42710)
                     /locus_tag="VFA54_15155"
     CDS             complement(42510..>42710)
                     /locus_tag="VFA54_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZS52204.1"
                     /translation="IVNLLDPDIVVIGGGVTKIGEPLFSKLRQTVPQRSVNPFAAQTP
                     IVPAQLENDAGIFGAAAVILSR"
BASE COUNT         8712 a        12927 c        12877 g         8194 t
ORIGIN      
        1 tggcgtggct cgccaccacg tgttgcgctg cctggaagcc aatcagccgt acgtgcctat
       61 tgcccggcaa ggctctccga gcgggcagga atcttcagcg cgggaacccg cggctcccgg
      121 ggacatcctc ggcgaactga cccggcatga gcgcatcgaa gccacgcggc gcgccgtact
      181 ctctctcccg ccgaattacc gggaggtggt tgtgctgtgc gatttgcagg aattgagcta
      241 cgccgaggca gcggcggccc tgggctgcgc gattgggacc gtccgttcga ggctgcaccg
      301 cgcacgcgct ctggtcgccg agagggtgac atccctgctg aaagattcgg tccggagcag
      361 gccggcttcc cctactgcga aagccgcaag gtgttttgta tgaattgcca ggacttcgaa
      421 gcgattgtga atgatctggc gcgggaccaa ttgattctcg tggctgaccg cgcccgggct
      481 ctcgtacata cggagacgtg tgcccgctgc gcggcgcgtc tggccgacga gcggatgctc
      541 acggccgaat tgcgtgcttt ggcagccggc tcggagtcgc aggaggcgcc ggccccggtc
      601 gaagaggcgc tgttgcgggc gttccggcgg cagaaggcgg cttccggggc ggtcgcagcc
      661 aaagggcgtg cgtgggtcgg ggcggttgcc gtgggcgccc tggccgcggc catgctgatc
      721 ctgctggccc tgacgattcc ggccttgcgc aggaatggac cgcccgaacc caaacccgcc
      781 gagggactcc gcgaaacaaa gccgccccgg aaacagcgga tagaagtagc cgaggcgcct
      841 gtgatagatg actcgatgct ggccggcgat gaaacggcgg cggcgcaatt gctgcccatc
      901 gatcttacag cgggactcct gaatagcgcg ctggatgtct ttgttccgct gcactatgga
      961 gaaactctca gcgacctcga tggggaccgc gtggtgcggg tcgaactgcc ctattcggcc
     1021 ttgatctcgc tcggagtttc tatggatgaa gatgccggca atgacggcat tgaagccgac
     1081 ttgctgctcg gcgatgacgg attggcccat gcgatccgct tgatccggtg agtagttctg
     1141 ctatggaggc aaacaggtag agatatgaaa gcgaggccaa aaataatcca caatttgacc
     1201 agatttttct tgctgctggg cgtcggggcg gccgtttttg cgcaaggtcc gccgcggcgg
     1261 caaggtccgg atatgggctt cccgcccggg cccggcggtg gatctgtgat aaggttcgtc
     1321 ggccaagtcg tcaagggggc ccctttttcc gggaccggcg tcacgacctt ctcggaaacc
     1381 ctgagcaatg ggaacaccat tacccgcgcg aactgtgtga agatctaccg cgacgccgat
     1441 gggcgcacgc ggcgcgaaga gacgccgaat tcttcggctt gcagcgcgac gccgcggttt
     1501 atcgtcatca cagaccctgt ggcgggcacg gaatccattt tgaaccctgc caacaatacc
     1561 tatcgccagt tcaagttgag gacgccgccc aatgctggag ccaatcgccc tcctcatgct
     1621 cccgggggca acgccgccaa cgtgcagacc ggaccggcga cgcctccgac gatcaccgtt
     1681 cccggcacgg cgctcgtcgc caatgggacg caaacgacga taaccatccc ggccgggcag
     1741 attggaaacg cgcagccgat cgtcatcaca tcgacccggt atttctcctc cgatttacag
     1801 gtggttgtga gcagtacgcg aatggatccg ctccgcggca acacgagcta taccctgact
     1861 aatgtctcga caaacgcgcc tgatccctct ctgtttacga ttcccgccgg ctacacccta
     1921 cagcaaggac cgggcggccg aggcttgggc aaagcgcaac aagagtggtt ccaccgcgac
     1981 ggccgttagc ccgcaaagcc ctttggtgca tttccgccgg atttgaaaac cgtttcccgg
     2041 ggctgaaaaa ggacaatcaa cccccgggtt gttttctggc cctgagtttc ccgctctcgc
     2101 tcttgctcta tttcgcggct acgctgctac gcgccgggcg aacggcagca tcaccggatt
     2161 caccgcccgg cggaagtcgg cgaagttcat gtacacgaca tcccgatgcg tgccggcgtt
     2221 gaaagcgatc gtatgctgct gcgccaggct gacttcggcg tatacaggca aaccgtacaa
     2281 gtttccgaaa ggaggcatcg cgcccagttc gcaatcggga aagagctgcc cgatttccat
     2341 ctcggttgcc aggcgcaggt gggtagcgcc cagagcgcgg cgcagctcct ggatgtccac
     2401 gatgctgtcg gctggcaata ctgccattac gaaagcgcca tcgaccacga ggacgacagt
     2461 cttggctatt ttccggctgc tcacgttctc ggcctgagcc acctcccgag ccgtgaaggc
     2521 gggagcatgc gcggagcgag cgaaaggaac gcgcaagtcg tccaaaaaat tttcaattct
     2581 ttctaaaacc ggcataagct gccctcccga atgcaaataa cggtggtctg gccgggcatc
     2641 ccgcgaggcg ctgatactga agggaattcg caaagagtaa gggatgcagg tttgccaggc
     2701 tccggctgcg gaacggtttg cgcaatcccc ggtcgccgaa acgcgcccga agagcagatc
     2761 gggcaaggga acatgcgctg catttttgtt atcgcgcctt tgccctgcgc cgtcaactct
     2821 cctatcgttt cgtgcgcagc aatcccggca ggaaaggtag tattagagat ggcggcaact
     2881 ggccccatga ggaaataatg aaacgcacag cgtctgctat ctggcaaggt gatttgaaaa
     2941 gcggaaaggg cataatctct accgccagcg gtgttttgtc gaacacgcaa tattctttca
     3001 gcgcccgttt tgagcaagga accggcacaa atcccgaaga gctcatcgcc gcggcccacg
     3061 ccggttgctt tgctatggcg ctgtcggcgc aactcggaga agccggtctg gtagcgcaga
     3121 gaattgacgc aagcgctacg gtaacgctgg aaaaaaccga cggcggcttt acggtcacaa
     3181 gcagtcattt acaggtcaca gtcaaagtcc caggaggcga tcgggcgaag attgagacgg
     3241 ccgccaacaa cgccaaggcc ggctgcccca tatcgaagct gctgaacgcc aaaattacga
     3301 tggacgcaaa atacgagacc tgatacttgc gtcccggaag ctatccgaga ttcgccgccc
     3361 cgctgcgcta gcgcgtggcg gttcctcctc tcaaaaacac ctcagaacgt atcggccttg
     3421 gcgtgggcca aactttcgtt ctggcccacg tgggccgctg cgtttcttgc tactcctgcc
     3481 ttctctactc cggccggcag ccgcgccggc ccctctgcaa gttcccgggc cgtcaccggc
     3541 ccggccgcat aagcatcctt ccaggctacg cggccttcga tacctctggc ctgacgggtt
     3601 gcaccatcgc caaccgagac tgagtccaaa gccacgaagc gctgatcgcc agtgtaatcg
     3661 tgggcagaat ccaaaggcct aaatcgccga aaatcatcaa agttcgcggg aagaagttgg
     3721 ccgcgtagat ctggtggtat ccgtagaacg agaccacaat ggaccagatc aagccggcgg
     3781 cgcttgccca gcaccacccc ctcaggccca gggcccttct ttgcgcgcca aaggcgtaac
     3841 ccaggaacac gaatccccag aaaccaaagt aggccaccag cgccgccatg taaggcaggc
     3901 cgaagaggcc ggcttggtag aggtattgca gtccggtttc ctgagttctc tggtattgca
     3961 ggaccagcat gtcatgcccg aaagccagcc aaacgttgta aatgcccatc agaaccagcg
     4021 gcacgccgag aaagcctaag ccggtgactg tggtcgtggg gtgcgtctgg tagatgcgcg
     4081 accccaaagc cagcaggaag aacaaacctg ccaggcaaac gcaaaatata aacgagtgaa
     4141 ggacaaaatc tccgccggcg cgcggttgca gcgatctcag catagcgctc cagtcggttt
     4201 tggcgtaagg attccaacgg gtgaagcgca gagtctcgac cagtcctacg atgaaaaaca
     4261 cgatcgctgt cagccagccg cacaggcttg caaagcgcac tggacgtgag tcacgaaaca
     4321 tacacacctc cctgtgagta cattctgttt agagggaaaa aacagtagaa cggcggagtg
     4381 gttgcggaca tcatagatcc actccctaat ctttttgtaa cactcttcac ctgtaaaatc
     4441 aatgcgcgcg gcttcgcagt cgggcgctgg gttccgcgcc gggctgaggg ggaaatatcg
     4501 tggaaaaatc ctgaagagcg actaccgaat gatcgaaaag ccgcgccgcc gaacgactcg
     4561 gccggtcctt taccgcagtc cgaactcctg caccacaatt cccaggcggc gctgatcacc
     4621 gggcgcctga aacgtcttgt ccaccgagat gatgacctcg catgccggcc ctggcgggag
     4681 ttttacgggc gcggaaagca agtagcgtcc cggagccgcg taagcccggt cggcgacctc
     4741 gattccatcc accgcaactg taattcgccg cgcgggagcc gcgtcgggaa tgaaaaacga
     4801 gatcgtaaac acggacgctc cggtctccgc cggcttgaga gcggcgacgg cttgaccctc
     4861 catccagcgc cacgcacggt cctcgacatc gtagaagccc gacaacaact gcggggccgc
     4921 tgcgggatca ttcattcgca aataggagag ctgcggctcg gctttttcca gaacgctcac
     4981 ggaaatgcga tccagcacgc cgcgcccgag gtcgaatggt aagaggccga atccgctcga
     5041 cgaatagccg gcgcgcgcgt ccaaccctat ggtctgcacc ggcaggctgc tgcgaatttc
     5101 cgcttgcgaa attctccgga gccgtcctcc ggagctggtg aagggaatct ttcccgcaag
     5161 ctcgctttcc accaccaccg agcccggata aacaggttgc tctttgtcca ggggctcagc
     5221 acccgattgc tcgagatagt accgcagtcc ccactccgcg cccgaccaga gggggcgtcc
     5281 tgtggcggat ttttgcagtt cgcgggccag ttctctatat cgctcggagt actgataatt
     5341 ggacgcggcg agcgtgaggc cgagcgacag attggctgcg gcggccgccc agagcagccg
     5401 cggccgcggc aagacccgaa ccagcgtaag cactaccggc gcggccaaag gcaataggta
     5461 tcgcgcggcg ccggcgtaga acaccaccac ggccccggca aaataaacca ggccccaagc
     5521 ggcgagaatc ccgtcctcgc tcgagcggcg tcgccataga tcacgtatcc agcgggtgag
     5581 aatggcggag ccggcgacca ggctggcggc caacaaggct cgctgccacc acaagtagcc
     5641 cttcacggcc agggcggcgg caacggccag gggcgccgga agccaggccc acagcggggc
     5701 ccagagcgcc gcgggcggga agacaatcca cccgaggtga accaccaagg ccgatgccga
     5761 tcgggccttt tgttcaaacc ccagaaatcc gtaactggag aggtagcccg ccaggatgcc
     5821 ggcaggaagg cggcctgagg tcatccgctg gaacacctgc cagcacgcga gcgtggcggg
     5881 agccgccgat gccgcaaccc aggcgccggt cgagcgccgc cgccgatacc aggcatggtg
     5941 agcaagaatc ggggcggcga atacggcctg gtatgcggcg agggccgcca cggccagcga
     6001 gcccgcggcc agcttctggt tgccctcaaa gtagagcgca aagcccgcca tccagaaggc
     6061 cagaaaaggc agatcggcct ccagagagtt tccatttaca acaaatgccg gcacggcggc
     6121 gaacagcaac gcggccagca aggggtgggc ggtgaaacgc cttgcgaggc gatacatggc
     6181 ggctgcggcg atcagcgaaa aagtgaggta aaccgtatga aacggcaatt cccgcacgcc
     6241 tccgaagagg tgaatcaaac cggccagaat ataggcgttc agcggcgggt gcgggtggcc
     6301 ggccatagaa acgcgagcgc cctgaaacgt gtagcccatc tgcatgggat gcagagggtc
     6361 cacgcgggcg ttgcgggcta tggcgagata gtagacgtcg tctccctgga tcggttgttg
     6421 aagaaaggga agacggatta atacgactgc ggcgaggaca acggctaacc gggcttccat
     6481 cggaccaccg gcttacgcgc cgccgccact tcatctaccc gcttgacgcg tgtgttatgc
     6541 ggcgccgtgc gcaccaattc cggatcctgc cgggcctccg ccgcgatcga ccgcagcgcg
     6601 tcgatcagca gatccagctc gcgcttgtct tcggtttcgg taggctcgat catcagcgct
     6661 ccctgtacaa tcagcgggaa gctcactgta tagggatgaa agccgtaatc gatcagccgc
     6721 tttgcaatgt cgccggtccg tacgccccga gccgcttgcc gggcgtcgct gaaaacgcac
     6781 tcatgcaggg aaggcgaggg atacggcaga tcgtaacagc cctcaaggtg cttgcgaacg
     6841 tagttggcgt tgagcactgc atcgagcgta gcattgcgca agccctctcc tccatgagcc
     6901 agaatatacg cgagcgcccg cacgagcacg ccgaaatttc cgtagaaggc cctcacccgt
     6961 ccgatcgact gcgggcggtc gtagtcgtaa tgcagccggc cgttgtctct ggcgatgcgc
     7021 ggcacgggta aaaacggctc aaactcgcgc ttcacagcga ccggccctcc cccgggtcct
     7081 ccgcctccgt ggggcgtgga aaacgtcttg tgcagattca ggtgcatcac gtcgatgccg
     7141 aaatctccag ggcgcgccaa gcccgccaag gcgttcatat ttgcgccgtc catgtaaagc
     7201 agcgccccgc gcgaatgcag cagtccggag acgcgggcga tgtcacgctc gaagatgccg
     7261 agcgtgttgg gattggtcac catcaatgcc gctacatctc catccaaatg ccgctcgagc
     7321 tccgccaggt cgaccatgcc ttcagcattg gaggcaatgc tctctacctg atagcccgca
     7381 atggcggccg ttgccggatt cgtgccgtgt gcggaatcgg ggatcagtac cttctttcgc
     7441 gcattgccgc gcgactcgag cagagcgcgc gccagcagga tgccggtgag ctcgccgtgc
     7501 gcgccggccg ccggctgcaa ggtcacggcg tccatgccgg taatttcagc cagcagttcc
     7561 tccaggcaaa cttgaatccg catggcgcct tgcgccaact gctccggctg atacgggtgc
     7621 gcctgggcca gcccctcaat ccgggctaca tgttcgttga cccgcgggtt gtacttcatg
     7681 gtgcaggaac ccagcggata aacgcctagg tcgatggcgt aattccacgt ggaaagcctg
     7741 gtgaaatggc gaatgatttc cacttcgctg acttcgggaa agcctgcgat ttcgcggcgc
     7801 acgttctccg cccccagcgc ggcggctgga tcaaccgggg gtacgtccag aggcggcagt
     7861 tgatatgctt ttttccccgg cgagctgcgc tcgaacagca gcggctcatt ctgattgata
     7921 tgggtgcgaa ctttccgaat cattctctta agcttcccgt cagcggcggc gcaagagcgc
     7981 ctaagacgac tccgtccgag gctgcgcctg gcgcagcgat ttaaagcagc gattcaaaaa
     8041 tgctcggcga gcctgtctat ctccacgcgt tttgtggttt cggtggcgca gagcagcatg
     8101 caatcggaaa gctccgggta gaaacgcccc atttccaggc cgccgatgat cttgcgctcg
     8161 agcaggcgcc ggttgatgtc cgccggattg cgctcgccgc attgcaccac gaattcgtta
     8221 aagaaaggtc ccgagaatcg aggcttgctg ccgcgcgcct ccaaagctcc ggccagataa
     8281 cgcgccttgg cgaggttctg ctcggccaat tcgcgcagcc cttgcttgcc gtaaacgctc
     8341 atgaagatcg tcgccatgag cgcgatcaga gcttggttgg tgcaaatgtt agacgtggcc
     8401 ttctcccgcc gaatatgctg ctcgcgggtg gccagagtca ggcaaaaggc ccggttgcct
     8461 tccttgtcga cggtctgccc gaccagcctt cccggcatct ggcggatata tttttccttg
     8521 gctgcgagaa ttcccacgta cggcccgccg tacgagggtg aaatggcgaa cgattgaagc
     8581 tcgcccgcga cgatatccgc atccgcgggc ggctgcaaca gccccagcga aaccgcctcg
     8641 gtaaacatca cgatcagcag ggcgcccttt tcgtgcgcaa tgcgcgccgc cttttgcacc
     8701 ggctcgacaa cgccgaaaaa gtttggagat tggaccacca ccgccgcagt ctgctcgttc
     8761 accaaagccg ccagccgccc cagatctaca ctgcctgttt gcttttcata gggagcctca
     8821 tcgacaggaa gaccttgatg ccgggcgtag gtccggtgaa cctcgcggta ttccggatgc
     8881 accgaacggg ccaccaccgc gcggtcccgt ccggtaatgc ggcaggccat catcaccgcc
     8941 tcgggcagtg cggtcgagcc gtcgtacatc gaggcgttgg ctacatccat gccggtcaac
     9001 tggcagatca tagtttggaa ttcgaaaatc gaggccagcg ttccctgcga aatctccggc
     9061 tgataagggg tgtaggcggt gagaaattca ccgcgcgaga tcagagagtc gatgatcacc
     9121 gggcgaaagt gatagtatgc gcccgccccc aggaaactgg agtagccgtt ggcgttctcg
     9181 gcggcgcgct cgcgaaaata ctcgatgatc tcgtattccg atttgccggg cggaatctgg
     9241 agcggccgct tcagccgcag ctcttccgga agatgcgaaa acaaatcctc gatcgagtcg
     9301 agccccatcg aggccagcat ttcgcggcgc tcggaatccg acttgggaag atagcgcatg
     9361 atcgcctgtt gtataaacga tgcccgcggc gcgccggagg attcaatgac cggtttcggc
     9421 gctgatgtat tcctgatagg cggcggcggt gagaagccca tccagctcgg cgcggtcgct
     9481 cagccggatt ttcaccagcc aagcggaccc gtgaggatct tcgttcaggc tctccggctt
     9541 ctcggccaac tcgttgttca cctcgatcac ttcgccgctc gccggcgaat aaatatcgga
     9601 taccgccttt acgctttcca ccgatcccat agtcttgccc gcgacaagct tcgcgccgac
     9661 cttgggcaaa tcgacataga cgatgtcgcc cagctccttc tgggcataat cggtgatgcc
     9721 gacgacgccc gtgtcgccct cgaccttgat ccattcgtgt tctttggtgt agcgaaaatc
     9781 ttccgggtac atgggcggtc acctcttgcg cgaataaaac ggcgtgggca cgatctcagc
     9841 cggcgccggc tgactgcgga tttcgatttc cagccgctgc ccgaccgccg cccatccggc
     9901 cggaagataa cacaggccga tgtttttctt cagaaacgga gcgggcgagc cgctggtgac
     9961 gaaaccggcg ggcgcgccgt cgatcatcgc caggtatcct tcacgtgcga tgctccggca
    10021 gcgcatctcg aacccagcca gccgccgggc gggtcctttc gccttctgac gcgccagcgc
    10081 gtcacggccg atgaaatcgc ctttgtctag tttcacgacc cagcttaacc cggcctcata
    10141 gggcgtcgtg gaagcgtcga tttcgtgccc atataaagcc atccttgctt cgaggcgcag
    10201 cgtgttgcgg gcgcccaggc cgcaaggccg gatggcaaat tcgcgtccgg cttcgagcaa
    10261 ggcgttccaa gcgcgatcgg cgaagtcggg cggcacgtaa atttcaaagc catcttcgcc
    10321 cgtatagccg gtgcgggcga taatggccgg cgttcctaaa atctcgccgc gagaaaacca
    10381 gtaataccgg atgccggcca ggtctgttcg cgtcagcttt tgcagcgtgg cgagcgccat
    10441 cggcccctgg atcgcgattt gggcgtatcg ttcactggca cattccactg cggcgtcgaa
    10501 tctgttttgc gcccggatcc actcgtaatc cttcttctga ttcgaggcgt tcacgcaaag
    10561 aaagtaatgc tgtgccgaaa tgcgatgcac cagcacatcg tccacgaagc cgcccgtcgg
    10621 gtacagcagg gcggaatatt gcgcctggtt gtcgtggagc tgcgaagcgg catttggcgt
    10681 gacccactcg accaaatcga gcgcctgcgg cccccgaatc tcaatttccc ccatgtggct
    10741 gacgtcaaag acgcccacgc cggtccgcac cgccatgtgt tcgctgatga tgcccgaata
    10801 ttcgacaggc atttcccagc cgccgaaatc gaccatcttg gcgcctagag cccggtgagt
    10861 ggggtaaagt ggggtccttt gtagggccgt tgtggccccc gcaatttcca taagcggcga
    10921 taggcaggcc gatgaaagga cactctaaca ttgccgccgc aggggtgtca aactacgccg
    10981 cgccggccat cttcggccag gctggcctag gtgggattcc gcttctatcc gcaaatcgcg
    11041 tttcactcgg ctgaagaaac ttgggtcgtg ggaagcgcca cggcctctgg gacaggcata
    11101 ggcgccaggg tgcgctacga tggtctcttg aacctcagcg agaaccgcca ggatagtgct
    11161 cttgcctgtg atgggaggcc ggcagtcgat ttctcattcc cccgagcaat cgggcggctc
    11221 tctccggcgg tgttccttcg aatactttcc tcgcaccgga agtgaagttg ccagatttca
    11281 ggattgagac gctgtgatcc gccgattagc cctgctcgtc gctgcgttgc ttttcgccga
    11341 tagcttgctt tatttggcgc tgcgcattct tgacagcagc cgcatctttt acgaccgctc
    11401 tccagtagac gcgcgtcggc tccactactg ggtccagaac agctacgatc ctgagcttgg
    11461 ctgggacgtc ggcaaaggct ccaagaacaa tgtcggctcg gctcggcgcg gggattaccc
    11521 tgcggcggag caatacaagt tgaaggcttt tggcgacagc ttcaccttcg gctccgacgt
    11581 gggcgacggc cagacctggg aagcagccat cgagcgcgag accggctggg cctgcttgaa
    11641 ttatggggtt cccggctacg gtcctgatca ggcgctgttg aagtatcagc gcaccggcgt
    11701 ccacacgcaa ttcacgattt tgggtgttca agaagagaac atcgcccgcg tcgtgaacgt
    11761 ttaccgggcc ttctacatgg aagattgggg acctcccaag ccccgctttt ttctggaagc
    11821 cggcggcctg cgcctggagc ccaaccccat tgcgaggact gaagatgcct cgcggctgct
    11881 cgatccggcg ttcgtggaca gcttgcgccg gctcgactac tggccgcgct acaacgagga
    11941 cgtgctggga gcgccgcggc ggctgcgatg gcccgccacc tggatcgtcc tcagccatgc
    12001 accgtttttc gcccgccgcg ccgtgctgga ggtccggctg cgctggagtc ccggctacga
    12061 agatgaagcc cgccgtttca aaccttatca cctctacgcc gaatcgtcgg aagcatttca
    12121 aatcctcgtg cgcatcattg accgtttcgc cgagttatgc gccgcgcgcg gagagcggcc
    12181 gctcgtgctg gtttttccca tggagcacac agtggatctc atgaagacct acggaaaatg
    12241 catttacgag ccgcttacgc ggcggcttga cgcgagcagc atcccccata tcgatttcgg
    12301 tccgatcttt gcgagagagg attaccggca gtattacgtc cgttacaacg gccatttttc
    12361 tgccgccggc aatgagcggg tcgcgcggga aatcattcgc ttcatcgaaa gcccaggggc
    12421 tggacacagc ggatcaaggt aacaagatac cgagggcgcc cgctctccgt ttcactgcgg
    12481 cgacgataag gtttggcgcg cctcgggcgc tctgggaagc cggcgctcaa atgaagattc
    12541 ttccgggggc cggcgttcaa tagtgcaaat accggatgtc tccatgcacg aagggcttcg
    12601 cgtagtaaat ctccgtctcc agcggcgcca gctgcgcctg ggaagcgaga cctccgctgc
    12661 cggaaagcgc ggcgaagcct ttgagcggca acacctcgta aaggccgggc cggcgctcga
    12721 tggcgacata acggcgcacc gtataaacgc cgctttcatt gcgggcgatg acgtgtaccc
    12781 gcgcggcgag ttcttctgcg ctggtgtccc tcatgaaagc ctggtggaaa aaccgaagcc
    12841 ggccgcgctc caccacgatc tcccgcgcaa agcgcaaacc ggacaccccg gtccaatacc
    12901 tggcgaaatt ctggccgcca ggctcgagtg cgagggaaaa gtgcggcgcc gttccgatga
    12961 cgatcggcat tcccggggcg ataatccaat cccccgggct gagatgatcc agctcgcgca
    13021 gcgccggctt cagggattgt gtggcgagcg attccggaac ccaacactgc tgaatctccg
    13081 ccgctgtaca caccgcgaaa tagccgcaga ggagagcggc ccagccgagc gccaggaggc
    13141 gggcgcggat cgcggcgctc caggccgccg ggagagccag cagcaggcac agaaaaaagc
    13201 tgaaaggata ataatggcgc agggtcttgg aattgagaaa ggcaatgggc gcgtaggcga
    13261 aaacccacat gccggcggcg aacaaaatga ggctgccggc gctttgctgc cggtctgcgg
    13321 acggcaggcg ccgcgccaca gccagaacca gcaggccgca tgccaggccg gcaagcagcg
    13381 cggcgctgga tattccggac aaacggtagt aagagtgcgg cgtcagaccg ctgagattta
    13441 tcaaatgcgt gcgtgtcata cgcaggccgg cggctccgta ttcgtaagtg agcgcaaatc
    13501 tctcttcatg gcctccgggg cgcctcaggc tttgtaggta ccagcggtac ccgccaaccg
    13561 ccgggacgac aacggccagg ttcaggagcg taacgagcaa gcccgtccgg agtcttgcgc
    13621 gcccgaaaaa acagatcccc agccagagcg gggaagcgag caacagggcc gacgccggcg
    13681 atccggagaa taaaaccaaa aggatcagac ccgactgtaa aactgccgcc cgcaccgtca
    13741 gcttgccggt ttcgtaggtc tttagcgctt ggagcaggta gagaaacagc cacaacatcg
    13801 ggagcacgaa gaacgtgcag ttgaccggcc agaagagagg gccgtgggct accggcgtca
    13861 acacaacaag cgcagccgcc agatatgcca tgcgggtccc ggcccgcagg cggcgcagga
    13921 gagcgtaaaa aaggcaggcg ttgcccgcgt gcagcagcag cacggctgcg taagcgattt
    13981 tgggcgcgta gccgaaagtc caaacaagca agcagttgag ccactggctg acccaaaaaa
    14041 gctgaaagcc gccataaagc cggttctcga gcagggctcg caatatatgg agatgccctc
    14101 gcaaacctgt tccggccgct tgaagacaga agttaaggtg cagccagtcg tcggccacat
    14161 attccacgtc ctgccagtag aagaggtaaa ccagggcgag caaaaaaaag gacagccggg
    14221 gaatccagtg cggcgcctgc cgctcggctg ccatgttcac gggattagga gcggcgcgcc
    14281 gcggcctgcc gggagccgcg cttcacgccg gtcgaagccc gggggccgcc caggctccca
    14341 gcgcctttcg ccggacagac agaccgattc gataaagagg ctggctgcat cggtggagta
    14401 gtaaatgaac agtgtacccg attttcttcc gtgctccgag ctcccgaggg cattccgcgc
    14461 ccgctccgct cgaggagccg cagacaaaga tcatcgggaa gccgccagcc gccgatttac
    14521 agacgcaggc gcggcagatc ggccgttctc cgcgccgtcc cgcgtttgaa acagagagcg
    14581 gccacgccgc acagggcctc ccggagtttg cgcctgtctt ccggagccgc taaaagatga
    14641 atttgagggc cagttgaatc tgccgggccg gaccggctgc tgagatcttt ccgaaacctg
    14701 aaccatttac cgaacggttg ggcgcctgaa cactgccctg ggagttcaat ccgaagtgcg
    14761 gtgtgttcat caagttgaaa aactcggacc ggaactgcac catgcgtccc tccgtgatgg
    14821 agatgttctt gatcatcgaa acgtcgaagt tcaccaagcc cggcccgtcc aaagtattgc
    14881 gaccggcgtt cccgaaggtg taaggcgccg ggggcgcgta ggcggcggta ttgaaccatt
    14941 ggtcaggggt tctttgagac gcgggcaaaa ccgggttctt gcccggcagc aactgcgccc
    15001 ggccgctcga gtcattgccc gtgttggccg gatctccgga agctgagacc gtgaatggca
    15061 ggccagtgcg ggcttgaaag atcccgctga tctgccatcc tcccacgagc cttccccgca
    15121 gcccgccgct gttcgcgaaa ggcttcccag gcccgaacgg cagctcatac agcgtgttga
    15181 agacaaagtt ctgccgggcg tcgaacagcg aacgcccgtg atcgattttg aggtttgtcg
    15241 gatcgatggg gcggttcgtc tcaccgctgg ttcttgtgga agaggcgtaa tccagcgatt
    15301 tgccgaaagt atacgagccc aggaacgaca ggccgcgcga gtattttttc tccaccttcg
    15361 cctgaagcga ttcgtaagtg gaattcaaaa tgtgttggat gaagaaaatg cgctcataaa
    15421 tcgggtaagg ccgccgtggc tgggccgctc cagggccggg tggcgccgtg ttgagcaggt
    15481 tcagtatgcc cagatgtgtg cctttattgg ccacatagcc gacttcaact accagatccg
    15541 gcgaaatctc gcgctgcaca ttgaatgtcc actgttgcat gtaaccggtc ggataatggg
    15601 ggtcggtcat ggtaagcgtc gggaccagaa cgctctgatt tccggccagg ccggaaaaca
    15661 ggttcttcat gtcgagctga ggcgtattag gatcgctggt cagtgccgcg cgcacattgc
    15721 ggggtacgtt cctggcgagg tcgaaaaacg gatttccgat gtcttgtgtg tagaagatgc
    15781 cgtacgccga acgaataacg gttttgggat tcaggctgta agcaaacccc acgcgaggcg
    15841 caaagttctt cttatccgcc tgcaacaggc ttcgtcccaa ccggccgtcg cggacgtaag
    15901 gaatgccatt caagttcacg ttcttcgggt aaaagaaata tggatcgccg ttaccggcgc
    15961 gaactatggt cggcgggttg gtggtaaggt ccagcgtctc gatgcgatcg tatttttcgt
    16021 aagggtaggg attatattca tagcgcaggc ccaaatttac cgtcagcttt ctcgataccc
    16081 tgacatcgtc ctgcacatag acgttccacg aattccgaaa atcgtagatc gaagtatcgc
    16141 ccacggagcc ttcggcggag tgaggcactc ccagtaagaa gtccgccatc ccggcgccgg
    16201 tcccgctggg gctggccggg tttgaggtga acagcgggct ggcgaaggta tacgatcccc
    16261 tgggcagatt gtcggcgaaa ttcgcgtatt gaaagcgccg aatctcaacg ccgacgttca
    16321 gattgtgccg tcctcttgtc agtgacagat tgtcgatgta ctggtacacg ttatctcgca
    16381 gcaacgaggg atagccgaag gtttgatccc cccaactggt gatgccctga accgccactg
    16441 ccggaatacc aaaatcaatc gggttgcgcg cgaggccgac aatccccgct tgcccgacgg
    16501 cgtcgagctt gaaggcgttt tgttgcagca gcgcattgct cagccggttg aatccgaaac
    16561 gaaactcatt gacggttcgc ggaccgaaaa catgggtttc gccgacggcc agcagttgtc
    16621 cccgcacatt cacgaaggtt ccgattcccg ggaaaactcc gggattgaaa gtagactcgt
    16681 cggaaagcgt gtagcgcccg aagatgttgt cgtttggccc catcttatgg tcgagacgcc
    16741 ccgtaaactg gtcgtcgttg gtgcttaaac cctcgttgct cagataattg ttggaggttc
    16801 caggaaggtt cggcgtaggg agaaattgca gcaggtattg ggatgtggga tccagcctgt
    16861 tttgcggtac ggcattgttg gggaacgggc tgcggacgaa gccgcccgct ttgctggggt
    16921 ccggctgcaa tgtcctcgga tcgtagattg tgactcccga tttcgagaaa tcgccagccc
    16981 tctggtccgg cgtcgccacc gtcgagacct gagtcagcgc ctggcgaatg cggaatcctt
    17041 cgtaatcaaa gaagaagaat gttttgtccc tgccgttgta aatcttcggc aggtagaccg
    17101 gcgccccggc cgtggcgcca aactggtttt gcttgaacgg gggaatgggt ttgtcagaac
    17161 ggtcaaaaaa gttcttggcg tcaaagtcgg agttgcgcaa gaattcaaac gctgtcccat
    17221 ggagctggtt ggtgcccgac ttggtgatga tattaatttg tccggcgccg ccgcggccgt
    17281 attgcgccga atagctgctg gtctgtacct tgaactcttg aagagcgtcc accgaaggac
    17341 tgacaatgta agtgccgaaa ttttcgtcag tgttcagcac gccatccaga acataattgt
    17401 tgaattcggt tcggccgccg ctgaccgcga tattcagcag cgaccgccgg ccgccttggc
    17461 ggctcgcatc gcgcgtgtac ccggaaatcg tgccgggaac cagagtagtc agttgcagaa
    17521 actgccgccc gttgagaggg agctcctcaa ttttgcgggt ttccacgact tgtccgacag
    17581 tggtggtttc cgattgcacc agagttgcgg cgccttcgac tgtcaccgac tcggtgacag
    17641 ttccaatttc cagggtcccg ttgaccgtgg cgacctggtt tacctccagc cggatattgc
    17701 tgtaaaccgc ctttttgaag ccttccctcg ttacgctcac gttgtacagg cccggctgca
    17761 aggccggcac attgtattcc ccgcgtgcat cggtctctgt gcgccgggtg aaattggtct
    17821 gtctattttg cacttctact gccgcacccg gtatggccgc cccagtcgag tctgtaataa
    17881 cgccggagat ggtccccgtc gcctcttgag ctggcagcgc ccccgccgcc agaagcagaa
    17941 atactcccgt tagcccgcac cttttgattg tcattgttcc tttcccccgc gttgtttcag
    18001 catccccggc aactccccgc ttggccggat tatacaccag cgcctttttc tccgctcgat
    18061 tttccgcgtc gtgcgcgcgg tgagaagcgc tgaggtttcc cgttgcctcg tcggcttctc
    18121 cgaacgcgga gcctccgcgc cagccccact gcactcgttg cttgcgtgaa ccaagtggta
    18181 acatagccct attctgattt accggggtac gggatttcac ggttgtagcg cgtcggcggc
    18241 gaagcctggc gtgagccagg caagcgtgaa gggatgcggc ccctacattg gcttcctcac
    18301 ccccctttta agctgcgtct agcaggagga aatgagtgaa catcaggacc gtttttgatc
    18361 gatcgttgcg agcgtgcctc gcctgtgcag cttgttttct ggcttttggc gcgcagggtc
    18421 cagcccaaac ccggcgcgag cgattcgacg tagtcatcat tagcggttcg tcggctggcg
    18481 tcggcgcggc tttgggagcg ggccgtatgg gggcaagcgt tgcgctgatc gaggacactc
    18541 ctgtgctcgg aggcatgctc gcaaacggaa tctccaatat cgacgcctat tcgctcgagt
    18601 cgctaagcgg cgtcttcgat gaatttcgcc tgcgggtcca agagcattac cggccgatta
    18661 tggcgagcga tcctatcttt cggactcgta tgaggatgcc gcgtcatgtg gacggcagat
    18721 cccgcgccgc caacgacgcg gaggccggag ggcgctggga accccatgtg gccgacgaga
    18781 tcttcaagga tatggtggca aaggttccca agatcacggt gttttacaac cgctggccga
    18841 caggcgtcat caagcagggg aatcgtgtga tcgccgtaaa caccgacgac gcgcgcggcg
    18901 accgaaagac gtttttcggg aaggtcgtga ttgatgccac gcacgaaggc gatatcgcgg
    18961 cgtgggccgg ggcggcttac cgcgtcgggc gggaagcgcg ctcgccgctg gaacctcatg
    19021 ccggggatat ctattttttt gacggcaccg gagaaattat ggacggcggc ggccagcagg
    19081 accgcgccgt ggcgtcctac ggtcttcgcc tgacgattca acactacagc gaccgccggg
    19141 attcatccca catcttgcag acgccgccgc ccggctatga caaatcgaag tacgagcaca
    19201 gcgcgtacga cgccgctatc accatgcccc acggcaaggc ggagatgaat gcgaacccga
    19261 tcggaaatga aatccaggag atcaactggg aatggcccga agccgcgcgc gagcagcgcc
    19321 ggcggctcta tgaggtctac aaaaatcgcg ctctcggctt cctctactat ttgcagcacg
    19381 agcgagggcg cacggacctg ggcctcccaa aagatgagtt taccgacaat ggcaacgttc
    19441 cctaccgggt gttcgtccgg gaagcgcgcc ggatcgtggg tgtgaagacg atgaccgaag
    19501 cggacatcaa cccgtttctc aacggccgcg gctggatccc gccggttcga gaagacgcta
    19561 ttgccatcgg gcactatcct atcgactcca agccggtgag aagcaaaacg gatttttccc
    19621 ggcccgataa gggtgccggc gatttttaca tcgtcaacgt cacaactcca ttccaggcgc
    19681 cgttcggcgc catcgtgccg gagaagattg acggcctgtt ggttcccgcc gctctctccg
    19741 ctacccatgt agccttttct tccatccgca tggaccccac gtggactgta ttggggcagg
    19801 ccgcgggcgt cgccgccgcc ctgagcgcgc ggttgaatcg gaacgtccgc tccgtccctg
    19861 tagagcagat ccagcgcgag cttctgaaac agaaatgcaa attgatgttt tattgggatc
    19921 tgccggccga ccaccccgct ttcgcggcgg tggaatggct cagcgtgcgc aacgcggtcg
    19981 ccggttatcc cgaccggctg tttcgccccg atcagagcct gacccgcgct gaaatggcgg
    20041 cgctgttggt caaggctttt cagctttggc ccagtgtcag cgacgtgcat ttcacggatg
    20101 tcgctcctac gcactgggcg tttcgggaga tcgaaacgct ttttgacaac cgggcgctgg
    20161 cagcttttgg catcagtccg cgctggcccg aagccggggc gtacgatccg gcaagagacg
    20221 aaggtttcgc tgcaagcaag cgctccggcg aattcctgcc ggcaaggccc gtgctgtgga
    20281 aggagttggc ggaaacactc tgggccatcc gcaagccgca tgacgagggg gaggacccaa
    20341 gctcctggat caggagcgta ttgcggggtt cgatgtttgg cgcgccctat gccggcagaa
    20401 attttcccgc cggggctgtg gttacccgag gagaagcgtg tgcgctcgtt gcttccctct
    20461 ctgacaacgg agccaactga ttcgcgtagt caccaaagtt cttcgggtcc ttcggggatt
    20521 tgtgcggccg gagaaagtcc gccggcccgc caagggcgtg aggcgcggaa aaactgctac
    20581 cccggcggac gtcgtacaat ggccggcaat gagcgtcctc tggagccgcc tgcttgggta
    20641 cgcccgccgc ctgggcgcgc cgctggttct gctctacgcg ggatcctctc tggccgctca
    20701 aagcgccgcg cctagcgcct atatcggcgc gagagcgtgc gcggaatgcc acgcggaccg
    20761 ggtgtcgcgg cagcaatctg ccggccatgc gcaggccctc gctccggcgg cggatcatcc
    20821 tctggcggct gccttcacgc cgccaaccat gttcactcgc cctccgaatt tccgctttca
    20881 attcctgaga ccaaagcaac ttcaggtgcg cgtgtttgat ggcgacaaca ccatcgagct
    20941 tcctgtggaa tgggctttcg gcgccggcgc gcaagctgtg acttttgtga cccgtgtgaa
    21001 tgagcagtgg taccttgagc actatttcag ctattattcc ggtcttcgtt ctttcgcgcc
    21061 gacgcccggg caggatgcca tccggccgag cagcctggtc tcggcggtgg gtctgctcta
    21121 taaggctctg gatccggata cggggatcgt cggatgcttt gagtgtcatt cgacaggacc
    21181 ggtcattacc gggtccgagg gaatccggcc gcgggagttg ggggtgcggt gtgaggcgtg
    21241 tcacggcccc gggagcgccc atgtccgtgc ggctaaagaa ggccccagca acgagattcg
    21301 aaaagcgatc cagaacccgg gccggctgcc ggcttcgcag ctaaacgttt tttgcggcag
    21361 atgccatcgc ccgccggctg cgaagggaac ggcgatcgat tggaactacg cctggaacgt
    21421 ccggcaccaa ccggtttact tcagccagag cgcctgcttc cggaaaagcc agggcgcgtt
    21481 gacgtgcctg acctgccaca acccgcacga gccgctccgg cgcgacaacg ccttttacaa
    21541 tcagcattgc ctcgattgcc acaaacactc cggcggagag ccgcaaatcc gggccgcagt
    21601 taaggcccat ccgccccgcc cggtttgcga aagccgccag cctgccaact gcatcgattg
    21661 ccacatgccg cgggtaagcc cgcaggccgg cctgcgcttc accaaccatt ggatcggcgt
    21721 ttacgctgaa ggggccaagc tcaagcccag aagcgagcaa taggggacca gccgaatttt
    21781 gatccggtct attcttgccg ggttcgcttc cggcctggag ccgtaggcgc aggcgtcatg
    21841 gatagagcag atattgctgg cggaccttcc ggaactcggc caaatccttc tgaaaactgc
    21901 gattcacctc gctgcccgtc tcgcctctct tcagccgcgc gccggcgtct tcattgccga
    21961 gcaggcgcat gatgccgtcc acgttaaaga gccctgggtg gaactggagc gtcgcgtgaa
    22021 ccaattcgag tcccatgcgc acggaatcga gcgtgtcgcg attgagcaat tgcacgtcga
    22081 cgccctcgca gtcctcgtcc ttgaacagat aggcattcgg ccggaagcgc cgtggaatga
    22141 agctgacgcc gggaagcttg aggctgttca gataggcggc tgcctctcgc gccttgaacc
    22201 agggcgcgcc gatcatttga aagggagtat cggtcccccg gccaacggaa acttgccggg
    22261 attcgagcaa acatgtcccg ggatagagaa tcgcttccgt gagattccgg atggcgggcg
    22321 aggggttcac ccattccaga ccggtttcgt caaaccacat ccggcggttc cagcctttca
    22381 tctcgatcac gtgcagatcg gcgcccaatt tcttttcgcc gttgtaaagg cgcgccaact
    22441 cgccgactgt catgccgtgg cggatgggaa ggcccggcat atacgcgacg aaagaaacgt
    22501 acttttcgtc cagccgcggc ccttccacgg cgatgccgtt gataggattg ggacggtcca
    22561 ggacaaagta gggaatgtga gcctgcgccg cggcctccag gccgtagccg agcgtggtga
    22621 tgaaggtgta aaaacgcgct ccgatgtcct gaatgtcata gaccagcgca tcgatatcct
    22681 tcaacatgtc ggccgtcatc ttccgcgtcg ctccatcaaa caggctgcga atggggatat
    22741 cagttgttgg atcaatgctc gacgggatat tcggatcgtc gcgcaggccg gtaaggccat
    22801 gctcgggtgt gaaaattacg gccaccttca ccttgcccga ccgccgcagg acgtcgatgt
    22861 tgcggcggcc gtccgacgtg aggccgctat ggttcgtaat gataccgatg cgcttgccgg
    22921 cgagcggggc aaaatcctcc tcgactaaga catcaaggcc cgtctttact tgcgcggtga
    22981 gcgcaggaag ggataagaac aggaaaagag cggcggcccc ggcctgcatc gccggtcgcc
    23041 gctggttctg atttgataat tttttcatcc agccttcttt ccccggcgcg atgattcaag
    23101 actcagcctt gcggcagcgg cggcgaaccg cccagttcat tggctgacta gtacacgcgc
    23161 tcccgcgagc cggtacgctt cgatcagcgc cccaacggcc gggtcgtgca ggggcgcaat
    23221 caggcgattc gtcccgcaag agctcagcaa tctgatgaat tcggagcgga gcatcccaca
    23281 gcggaatacg ccgccgacgt aagcgatggc aacggactct tcgcgccgga agagccgcgt
    23341 ccgaaccaca tttgtaatgg ccgccaggtc tctggcggcg ttctcaagaa tcgagcaggc
    23401 caccggatcc tgcaatctcg ccgcttcatc caccaggagg gccagcgcgg cgatccgttt
    23461 gcgagggaat tcggcgctgt aaagtttccg ctgcacgagg cgaatgtcat tcaatcccac
    23521 cgcctcgcac aacatccggt gcagcgctgt ggaaggcccc caaccctctt cgaatcgcag
    23581 cgcggcgcgc agcgcctggc gcgccagatc gaaggcgctg ccttcgtcgc cgaacaggta
    23641 accccagcct ccggcccgtg ctgtttctcc cgcctgattc cgtccgaagc tgatagaccc
    23701 ggttccggcg atcgtcacaa cgccgggttc gccgcccagt gcgccggtca gcgcaattac
    23761 ggaatcgtcg ctcagcgcga tttgtctggc gtgaatcagt tcttctacga tcgcccgctt
    23821 gccggcatca gccccggtga agccgaaaaa ggccgcctcg aaggatgtat ccgcgagatc
    23881 gcgcccgagc tgccggcagg ccgcgcctgc ggattcttgc aaggcgcgaa acacccgctc
    23941 ccggccctga ggagcgccga tatgctcgac gggtccccct tcgccggtgg ccaggatgcg
    24001 ccctgattca tcgccgatta cagcccgggt ggaggactgg ccgccgtcaa ctccgaggaa
    24061 taaagtcaca ccgccgctcc attgacaact ttccaatcgg caagaaaatg ccggtatgcc
    24121 ccccacatca ccagcgccgc gcccgccagg taaaccagcg acagggcgga gaaaccgccg
    24181 gcgaggccca ccgtcatttt tgcgcgagcc agcaagaccg gagcgagaga agcgatgaga
    24241 aacgcaaatg cgatgagcaa tccggaggca gaggcgtggt aacgcggcgc gatgaccgaa
    24301 taaaacgctg gatacgtgtt cgaatcgaac aagccgcgga aaaatccgaa accggccagc
    24361 gccgcgtaca gcaggccttc ggacggagcc aatcccagaa agtagagaaa cggggaccca
    24421 gcgagcagag ccaggccctg gatctcgagc cgcacctgcg ggcgccgggg agcccaatga
    24481 tccgacagga tgcctccaat catcacaccc aggaacgcaa acaggtgatg gaacaacatg
    24541 ctggccagcc cagccgccgc cagtgacacg ccgaagcgtt cgtaaatgta cgtcggcatc
    24601 cacgccagat agcccacatt tacaaaaatc atcgcgccgc tccccagggc ggcaaagcgg
    24661 gccgtcggtg tcgagaggat agctgccaca gtctcgcgga cagccgcccg gcgctccggc
    24721 gcaggagaac ggctatcgcg cagccggaaa aaggaaacga gagacagcag aatcccgata
    24781 gcgccgaaaa cccaaaaggc ggcccgccag ccccaacggc tggcgatggc gcctcccatc
    24841 cagccgctcg cgatcaggcc cgtatacacg gcggtctgaa ataaggataa cgcgcgcgcc
    24901 cgggtttccg tgtgttcggc ggagagcata gcgaatgccg agggagcgaa aaacgcttca
    24961 ccaaccgctg tggctgttcc ccggacggcg accagataaa gcagcgtagc gccgaatccc
    25021 gtgcagagcg tggccaggct ccaggccagc aggcttacga ccacaatcgc cttccgcgag
    25081 cagatgtcgc ccaggaatcc ggctactgga accattaccg ccagcgccgc ggtgaaaaca
    25141 gagcccacca gtccgagctg caagtaggaa aggccgagat cgctgcgaat gctgggaagc
    25201 actacgctaa aaacctgccg gtcggcttgg ttgaaaaaaa atgccagcca tagccagagc
    25261 aaaagctccc agcgatagct catagacagt cccgagaagc tgcggccggc cggagcccgg
    25321 cgccattttc gagcgcgggt gcaagcccgt tcatgaggcg ggctccggtt ccgaccggcc
    25381 ggccccagct tgtgagaccg cttctgcaaa acgccgggcg attgtgaccg ggcgcgtgat
    25441 agcgctgccg acgacaacgg cataagcccc cgcgtccagc gctgcacgcg cctgttccgg
    25501 cgtttcaatc attccttcgg cgatcaccgg cacgggacag cggcgaacca gttcccggag
    25561 gaaagccaga tcgaacgagc gcacgtgcgc cgtctcctcg gtgtagccgc gcatggtcgt
    25621 aaggacaaaa ttggcgccgg actgtgccgc ggcgagagct tcctcgaggg tcgctatgtc
    25681 tgccagtgcc ggaacgttaa ggtcccgccg gatgcgcgcg agccggccaa gcgctccata
    25741 gcgctggccc cggcaggtga catcgatcgc caccatgtcc gccccggcgg cgatcagcgc
    25801 ttgcgccgcc tccccgctcg gcgtgatgag cggccttccg tcctcctgag gctgtttcca
    25861 gatgccaaga atgggcacgc tgacggcttt gcggatttcg cggatatcgt gcggaccgtt
    25921 ggcccgaatc gcagcggcgc cccccgctac cgcggaaacc gcgaaacgag ccatgcagtt
    25981 tggatcgcga aacggctcgc cctctcgggc ctggcacgag acaacgagcc gccggtaaaa
    26041 atggcggcag atgggcggca agtcctctct catgaattgc cccgcctaga aaccaacagc
    26101 atatcccagc gaagcggccc agagcgcaat ccggaatcgc ccggcgcaaa agggacatgc
    26161 tgccggcggc ctttctgctt ggaagagcac tcctcagacc aggccgcggc aagcgccggc
    26221 gcgcctcaga tcgcttcgga tttctgccct aacgcgactt cgagttgcta tccgtgaacc
    26281 gcggcccgga tgtcagaatc gcagcttgag cccgaactga atcacccgcg gcttcggcgt
    26341 aaacgcagtg gcagtgacct gaccgaaggt cgggcttccg tacatggtgc caggcacctc
    26401 gaaggttggc gtattggtca ggttaaacgc ctctgcccgg aactggaact ggtatttttc
    26461 acgcaccgaa aaattcttga acgccgaaaa atccatgctg aagaggccgt cgctgagaac
    26521 gtcgggcaag gtccggctgg cgtttccgta gctgtacggc gctggcgccg tgaatgctgc
    26581 tgtattaaac cactgggaaa ggctctgcga ggacgccttg tcgctcacgc cggccacgac
    26641 actgggccgg tttcccacgc cggtcagatt ggcgccgagc ggaatcggca ggccgctttg
    26701 cagcgtcgtg attgcgttta actgccagcc gccggccacc gcgcgataca gcggattgcc
    26761 gctcttgcct aaaggaacgt cccatagagc cgtcaccacg agccgctgtg gaacgtcgta
    26821 aagactcttg gaacgctccg cagaaagatt gttccagttc tgcaccaggt agccgctcac
    26881 gccgcccgga cgaacctgcg tcaaattgtc tcccagatcg agcagcttcg acgcggcata
    26941 agaggcgagc agggaaaagc ctcgcgaaaa gcgcttctcc accttcaccg tcagggcgtg
    27001 atagatggag ttgttcaaat acgagatcgg gctgatgacg ccgccttgcg aggtgggaag
    27061 ggaagtatag gcatactgcg gaaacggaag catcaaccag tattgcggca cttgcgccgc
    27121 ggccagcggg ccgctggtga tgactccgta gaacgggttg gggaccgtct ggcccagcgc
    27181 cgagcccaag ctgagggcct gattcggcag ccaatcgata tcctggttgt acatgaacaa
    27241 gtgcacgccg cgattgccca tatagccaac ttccaccagc cagttctccc atggctggtg
    27301 ctggatcgtg aaattccact gctcggaata gccgcggcct tggttataga gctggccggt
    27361 aacacccgtg ccgatgcccg tcagcgctcc cagcgaggcg ccggtgggcg gagtcaagcc
    27421 gttcgggaac gggttgctca gggtgttcgc cggagtgcgg ccgccgtcgt tggatgtgac
    27481 catcggggtt gtgctgctga atccgatctg agaaggcgtg tactggccct gcgtggtcgg
    27541 cacgaacatg atcccgaaac cgcctcggag aacagtcttc gcctggagtt ggtaggcgaa
    27601 gccgaagcgc ggttggaagt ccttgctggg gctggtgtag aaagctcgcg gatgaccgtt
    27661 aacgcccgag taaaccagcc cgccccgcag cgtcaagccg ggcgctttca gcggagaggc
    27721 tacatccgga tcaaaattct catagcggtt gtagcgttcc gtaggcgcgc cgtccttatc
    27781 ccagcgcaaa ccgagattca aggtcagctt gcgggagacc ttccagtcgt cctgaaaaaa
    27841 cagcgcgttg tatcgcagcg tgacggcgct ggaggcgttg atccccgcaa atgcactggc
    27901 cggcgtcccc agcagcaggg aggccacgct gtatccggct gtggcgctgg cctgatccgg
    27961 gttgggaccc tgggtgaagc cgcgattgaa agtgtaggaa ccaaccggcg agttcagagt
    28021 aaattcgttt cgctggtaga ggcggaactc gtagccgaat ttccaggttt gcgagccgcg
    28081 gattttcgtc agcgtcgcgc tggccgaccc ggtattggtg tggttcgccg ccgccgccgt
    28141 tccgccgaac gtggtcagat cgctgacgga aagacgcggg aacacgccct ggggcgcgtt
    28201 gggagcgttc tggctctgcg cgagcaggct tgcgggcaga cccagctttg tgatgtcaaa
    28261 accttggctc gggatgctgt aggcctcggt ctggtggctg aagccgatgc ggctatcgaa
    28321 cagcagcgtg ggcgagaaac tatcggtgta actgataaag gcattcctgc ggggaatgga
    28381 aatcaaacgg ccgtcgacgt cggctatgtt attgaagaag ttcgcaaatt gccaggcgag
    28441 ccggtcgtct gtgaaccgcc cggccaagcg cttcgtcggg ctgatattgt aatcgagccg
    28501 aatggtttcg gtgtctttgt tgattggcgt cgctccggtg aggaaaagat tcgacgaaag
    28561 gcccggaagg tttggctggg gatagtaact caaaatgttc tggccgacga ggttgatacg
    28621 gtctgccgga atcttgttgc ccggaaatgc agaacggatg aacctgccgg ggttgctcgg
    28681 gtcgggaacc gttgtggtcg ggtcgtaaat agtgatcaat tgccccgtgc ttgtggtcag
    28741 cccggaaaaa tctccggccc gctgcgcggc cgtgggtgag gtaatggtct cgatgctggc
    28801 gctgcgctcc cggtagcctt catagttgaa gaagtaaaac agtttgtttt taataatcgg
    28861 cccgcctaag gttcccccga actggttcac cttgtccggt gcgatcttgc ggccggcttt
    28921 gttggcgaaa aagtcattgg cattgagctt atcattgcgc aggaattcga aaaggcttcc
    28981 atgcagttcg ttggtcccgc ttttggagat catgctgata acgccgccgg tcgttcggcc
    29041 gaactccgcc gacatgctgt tggtctggac cttgaactcc tctgtgtcat ccaccgtgag
    29101 gaaggtcatc gggcccgagt ccaccatctt gtcattggca atgccatcca ccaggtaccc
    29161 gttggtcaga ggctggccgc cacctatgcc caccgcagcc aggcgccagc ttgagaggac
    29221 ctgacctccg aaatatccga ttcctcgcac agtcgggatc aggttggcca agcccatcgg
    29281 gttgcggccg ttcagcggaa ggtcttccac tttcttctct tccataacgg tacccaggga
    29341 ggaggtgttc ggctcgagca ggggcgttcc cccttgcact tccacagttt cagtcacttg
    29401 cccgatctgt aacgcgacgg gaacggccag ggagcggtcc acctcgacaa taattccgtc
    29461 ccgccggaac agcttgaagc cgggcgctga tgcctccagg gaatacgtgc cgggcggcag
    29521 cagcagaaag cggaaattgc ccgactcatc ggtgatggcg ttctcgacgg ctccggtacc
    29581 cgtattggtg agcttcacct gggcgttggc tacggcagct ccggccgggt ccatcacggt
    29641 tccggttata gagccggtga tttgctgggc gaaggaaaga gttgccgcca acagcgcaag
    29701 caagactccc cgcaagacag aattagcgga catgacacaa cctcctcgaa aattcgggcc
    29761 cctgccctac acgggcataa gtgtttcttc aactatacag atagtcggcc ccctgggcaa
    29821 gaaagaaaac tgactggctg gcccgtgggg taccggccct ctggcccccg caacggtatt
    29881 accggccgta ctatagacag tccgttttga ccgtcaacgg ttctcgtgaa tggctccgaa
    29941 cgggtcagag ttgattcgga ccctcggggg gcctccgtcg cgccgtggtc gtcctggcgg
    30001 ttccccgtaa acgcggcctt cggacctcgc cattgccgag accataaaat tccggacgaa
    30061 ctgcttctcc aacgacactc tccggcctcg aaattagggt gatatttttt gaaattttga
    30121 ttgttttatt ctaatcactt tttgcaattg acagagcagt actcgggatg tataagggag
    30181 cgaaatcaac catttctagg cggacgaagg gggtaccgca tgtttcgttc ccatcggcgc
    30241 gggtttctgt cgagcctgtc ggccctgttt ctcctcgctg cactgatcgc tttcaacgca
    30301 gcggctttcg gacaattgat caccggcacg gtgacgggct ctgtgcagga tgcgaccgga
    30361 gctgccatta tcggagctac ggtaaggctc accaataccg gcactggggt cgtacagacg
    30421 acgcaatcgg ataattcggg aaatttccag tttttgctgc tgcctcccgg cacctacact
    30481 gtggaagcaa ccagtgcggg cttccgcgct ttccggcggg aaggaatcat tgtcgaagcg
    30541 gaccgctctc tggccgtgcc ggtggtgcta agcgtcggcc aggtcaccga gacggttgaa
    30601 gtggtcgggg gcacgccgct gctcgagccc aacacctcgg aggtcggcac gactgtcgac
    30661 agccagaagg taatcgagtt gcccctgaac gcccgcaatc cgatgggcct ggcaaacctg
    30721 atcccgactg tgaaaggcgt ggggtacttc gggcagcaga ttctgacgtc gtggcggatt
    30781 ggggcgatca acatcggcgg gggccagccg ctgaccagcg ccttcctgct ggacggcgtg
    30841 ccaaacgaca aattaggaga cgcggccggc gcgaatacct atctgacgac ggattccacg
    30901 caggaattca aggtcattac caattccatg tcggccgagt acggccgcac cactggcggc
    30961 gtcatcagcg tggtgagtaa atcaggcacc aacgaatatc acggcggctt gttcatgtac
    31021 gtgcaaaaca cggcgctgaa tgccaacgat ttttttgcta atgcctcggg cacgccgctc
    31081 gcgcccgtgc accagtacca gttcggcggg tcgctaggcg gccgcatcat caaggacaag
    31141 ctgttctttt tcggaaactt tgaaggattc gtgcaacacc tctcggcaag cgaagtcatc
    31201 aactcgccga cagccctcca gcgggccggc gatttttcgc aaaccttcgc tgccaatggg
    31261 caactgatca cgatctacga cccgctgacg acggcgccca atccgaataa gccgggcgct
    31321 tcgattcgaa cgccgtttcc gggcaacgtc attcctccca accgaatcag ccagtttgca
    31381 aagggttttt ttgcgctcta ccccttgccc aatttgcccg gccagaaagt aacgggaacg
    31441 cagaatcttt tcctgatcgg caacataccc acggatcggg aaaccggagg agtgaaagtc
    31501 gattacatgc tcaatcagaa tcgccggctg gcggtgcggt atacgcgaga tgtgctgaat
    31561 gaatcggtcc cgaacggcag ctttttccag aatgtgctgg acaacgacaa gaaaatcatt
    31621 tacgttccgc gccacagcgg ctctgtttcc tacactgaca gcctcagccc cacgctgctg
    31681 ttggatctca ggtccggaat caaccgggac tatgaccagt ccattccctg gagctatgtc
    31741 gggccgtacg aaaagacggg ctttccgctc acttccctcg gcctgcccca gtcgctcgtc
    31801 aaccaactgc aacctggcgc ggtgcaattt ccggctttga ccatttccga tctgggaggc
    31861 attctgggcg gctatggatc cgctataggc aatcgagccg cgtacacatg gggcaacgtc
    31921 gcctcgatca cgaaaatcat gggcgcgcat acgatcaaga cggggtatca gctcaccgtt
    31981 taccggggct ttcctctgga ccggactcca ctgaacttca cgtttactcg cgggtttacc
    32041 caaggtcccg atcctaccgt cgccagcgcc tcggccggct atggtacggc ctctctggaa
    32101 ctgggcacgc cctcggcggg cacggcaact tatcagccga gccatgagca gcaggaaatc
    32161 gatcacgggc tttatgtgca agatgactgg aaagcgaccc agaaactgac tctgaacctc
    32221 gggcttcgtt gggaatatca aggtccattc acggatcgct ataacgagat gaccaatttc
    32281 gacccgaatg tcacgtcacc gctgcaagtc ccgggcctga cgctcaaggg cggcacaatt
    32341 tttccgggcg tcaacggtgt gccgcgggga gtggtggatg tttcctataa gcacttcagc
    32401 ccgcgagtgg gattcgccta ccaaaccatg aacaagctgg tggcgcgggg ggcttacgga
    32461 gtgttttttg ttccggaaaa aggcattctg aatcccgctt ccactggttt cggcatcgtc
    32521 acaaccatgg ttgccagcct ggataacggg ttgacgcctt acaatacgat caacgatcct
    32581 ttcccggatg gcctgctgtt tccgagcggg agcagccaag gcctcctcac cggaattggg
    32641 accagcattt cggggcagtt gcggcatgtg ctgcccggat atgcccagca gtggaatttt
    32701 tcgttgcagt actcgccctg gaacaactgg ctgatcgaag gcgcgtacct aggcaataaa
    32761 gggactcatc tggagacgct ccagggactc aatctgaacc agatcaaccc gcagtacctg
    32821 tcgctgggca atgctttgaa ccagcaggcg ccgaatccgt tctatgggat tatcacgtcg
    32881 ggcccgttga gcgctccgac catctcccgg cagcaattgc tgctgccgta tccccaatat
    32941 acggctgtca acgggggttg gtcctacaac ggcaactcga tttatcacgc ctttgccttg
    33001 aaagtagaaa agcgcttttc gcaaggcttc tccattctgg cctcgtatgt ggcgagcaag
    33061 atgattgacg gggcggtcgg ctccggaggc gccgttcgca ctggcggcac gccggaaaca
    33121 ggcattgtga actggtacaa cttacgcgct gagcgctcga aaagcatcta cgatgtaccc
    33181 cagcgcgcgg tcattacggt cttgtggcag gaaccatttt tcaaaggcag ccacgggctg
    33241 acccgccagg tgctcggcgg ctggaacgcg aacgggatcg tcacgattca aagcggccag
    33301 agcatagcgc tgcaatcagg gtctgcgtcc cagcggccca atgtcgtctc tggcgtagac
    33361 gatcatgccg cccaacaaag tcttacgcag tggttcaata aggcggcatt ctccatcccc
    33421 gcgccattta cttacgggaa cgcctcgaga accattccca acatcatgag cgacggcatg
    33481 tttgacatgg attttgcgct ttacaaagac tttgcgctca gcgagcggtt caaattgcag
    33541 cttaaaggcg aggcatttaa cctgaccaat acgccgactt ttgacgtgcc caaccgggat
    33601 gtgacgtccc agagttttgg ggtagtttca gctacggctt taaatccgag gccgagatcc
    33661 gtacaattgt cactgaggct ggcgttttag gatacggctc tctgctgcca tgctaatacg
    33721 ttctgctgtt aagctgtctc agcggcgctt ctggctgacc ctttgcgcgg ctttagcggt
    33781 ttgtataccc tgggtgcgca gcctccgcgg gcaaaacgcc agcggggccg gcatccgagc
    33841 ggatgcaact tcctaccaaa aggaaattca accctttttg gcgaaaaatt gctatctctg
    33901 ccacaacgaa aagctgaaca cagcgaacct gaatctcgaa gcctaccggg atgcaggctt
    33961 ggcgttgcaa cagacgcagg tgtggcagaa agtgttggag aaactcagcg ccggcaaaat
    34021 gccgccgccc ggacttccgc gtccggaccc cggccaggtc aacacggtca cagcctggat
    34081 cagcgggttg ctccacggga cggaaacgcc agcgaaggta aatccgggcc gcgtgacggc
    34141 ccggcggctg aatcgagtgg agtacaccaa caccatccgg gatctgctag gcgtgccgtt
    34201 tcaagcggca gggaagttcc cgaccgacga ttccggctat gggtttgaca atatcggtga
    34261 cgtgctctcg ctctcgcccc tgctgatgga aaagtatcta tccgccgcca agcaaatttc
    34321 ccgcgaggcc gtttacgggc ccagccttcc tcccaagccg actctgctgg tccgcctcat
    34381 ggcgaagaaa gcgcaggata ctggcggctc gctttcgggg gaaaacatcc tcccgtactc
    34441 catgcgaggc gcgttgtacg gcacgtactg gttccccgtt gacgcggaat acgagttccg
    34501 cgtccgcgtg gcgaattacc gtggccagga cccgaacgat cttcccccgg agcggagacg
    34561 gcgtttggag gaaatcagaa ggggcttgcc ggttggccag cagcaggatc aagagcaggc
    34621 gcagccgccg gccgggccag cggccgcgcc gaaagcctcc cggttccgcc ggcctctgac
    34681 gccggaagaa atccaagcac gcgaggaaga atcgagaaaa gcttttccac cggtaacgat
    34741 ggtcatgact ctggacggca agccggtgct gaccggtgtt gtcgaaggcg acgttaacta
    34801 ccagtattcc cgcggcgagc ttgtgggccg gacgcacgtc aaggcgggcg cacattttct
    34861 gcgcgcttct tatccggagc tggccgactt gccggacccg cggaaaaatg tcaatcccga
    34921 tggccgccgc aagctttatg tcgactacgt gaacatcatc gggccgttca accccagcgc
    34981 cgagccgccg gagagctatc gacggatatt tgtctgcggc catgcgcccg gccggcaccg
    35041 gccggagtgc gcacggaagg ttttgcagaa tctggccact aaggcctacc ggtgtccagc
    35101 cacaagcagt gaggtggagg gtctcctgcg gtttgtgacg ctggcgcaga aacaagggga
    35161 ttcattcgag gaagggattc gcgtcgcgct cgaggcgatt ctcgtttcac cgcattttct
    35221 gttccgcatc gagcgggatg cgaatccgag tgacgcggct tcgccgcacc cggtcagcca
    35281 gtacgagctc gcctcgcggc tatcgtattt cctttgggcg agcatgccgg atgacgagtt
    35341 gctgcgactc gcccgcgagg agaaattgcg ggatccacaa gttctcgaat cgcagatccg
    35401 gcggatgctg gccgacccga agtcagagag cctggtggat gacttcgcct cccagtggct
    35461 gcaacttcgc aatctggacc gcgtccggcc ggactcgaag cgcttctcga ctgtcgatga
    35521 cgagctgctg gacgctatgc gccgcgagac caagctattt gtcgatgcca tcattcacga
    35581 ggaccgcagc gtcctggatt tcatcgatgc ccctttcacc tttctcaacg ggcccctggc
    35641 gcggcattac ggcgttccgg gggtcacggg agaggagttc cggcgcgtca atttgagcgg
    35701 cgaaatggcg gagcaacggg gcggtgtgct gagccagggc agcgtgttga caatctcctc
    35761 atacgcgacc aggacttccc cggtgctccg gggcaggtgg gtgctggaca atcttctggg
    35821 cacgccgccg ccgcccccgc cgcccaacgt accgcagttg aatgaagctg cccttggctc
    35881 taccgcctcg atgcgcgagc aactcgaggc gcaccgcaag aatgcgactt gcgccgtttg
    35941 ccacaaccaa atggacccca tcggtttcgg gctcgaaaat tacgatgcca ccggagcttg
    36001 gcgcactgag gacggtaagt tccccatcga cagctcgggc gttatgcccg gcggcaaatc
    36061 cttccaaggg ccgaagcagc tcaagcaaat cctgctggga gagtcggatc tgttcgtccg
    36121 gaatctcaca gagaagatgc tgacctacgc gctgggccgc ggcctcgagc cctatgacga
    36181 gccggtggtg agccagatca gccggcgctt ggccgccgat aattatcggt tttctacttt
    36241 gatagcggaa atcataaaaa gtacgccctt tcagatgcgg agcggagaat ccggggcgcg
    36301 cggcaggccg tcccgtacgg accagcagcg tgtcgccggc gattattcga agacccgcat
    36361 ctccgggccg caggagcgca aggatgaacc ttagtaagaa actagcgcgg cgcacattcc
    36421 tgagagggct gggcacttcg atcgctctgc cgttcctcga ggccatggtt cccgcctggg
    36481 ccgcgacgtc agccctgaaa tctccgtgcc ggatggcgtt tctttatgtg ccgaacggca
    36541 tcattatgga ggcgtggacg ccgcagaccg aaggcccggt tgctgaactg ccgagaacgc
    36601 tgcctcgggt cctggagccc ctggcgccct atcgcgacaa cctgctgctg ctatcggggc
    36661 tgagtctcaa aaacggcgat gcgctggggg atggccccgg agatcacgcc cgcgccgggg
    36721 ccagttatct gacggccgct caccccaaga agactttcgg cgcggacatt caggttggcg
    36781 tttcggtgga ccagatcgca gcgcaaaaga tcgcgggaca aacccagttc gcttcgctcg
    36841 agttaagctg tgaagacgga ctgcttggcg gcaattgcga caatggctat agctgcgcat
    36901 acagcaacag catctcctgg cggacgcctt ccacgccgat gccggcggag atcagccccc
    36961 gcgccctttt cgagcgcctg ttcggcggcg ccgatgctag tgaaactcca cagcggcggg
    37021 ccagacagcg cctctacgaa aaaagcattc tcgattttgt cctggaagat acgcagcggc
    37081 tgaagggaac gctcggtccg accgaccggc gcaaaatgga tgaatatctc tacgccgtgc
    37141 gcgatatcga aaagcgcatc gaaagggcgg agcgcaacaa caggcaagct cttccgaccg
    37201 tcgtccagcc gccttcaggg atccccgaaa gcttcaaaga gcattccgag ctcatgtttg
    37261 acctgctcgc tgtcgctttt caggccgact tgacccgcat cgccacgctc atgctcggcc
    37321 tggagcaaag cacacgcagc tatccggaaa tcggcatttc agaggcgcac cacggcctga
    37381 cccatcacct gggcaacaaa gagaagatcg aaaaggttac gcagatcaat tgtttccatg
    37441 cccggcaatt cgcgtatttt ctgggcaagc tcaactcgat caaagacggc gacgggactc
    37501 tgctcgatca ctcgatgatc gtttacggaa gcgggctcag cgacggcaac cggcacttgc
    37561 atcacaatct gcctgtgctg atggcgggcc gcggctgcgg aaccgttcgt cccggccgcc
    37621 atgtaatcta cccgcaagac acgccgatgg cgaatttgta tgtcgccatg ctggaccgaa
    37681 tgggagtgca gatcgagtcc atggccgaca gcaacggcga gctcggctat ttggaaggca
    37741 tttgagccca taaagctgcg tttgaggagg ctcctatcgc agtccgcagg gcccagccgg
    37801 ctgcggcacc ttgtgttacg ggccacgttg cgcctcgaac caatgggctc tcggccgccc
    37861 gcggcagagg cggatgcccg cgccagttaa ggtccgatcc aagtctggca aaagcataca
    37921 attcccgaat ccggacttgg cgtccccctg gcggcgggag catttaccag tgctaaattg
    37981 ccgttccttg cgccattttg cggttgcaat ttcgctggtt cgttttatac taacaaccgt
    38041 ttatatactg gtgactgctc ctggcgtgtg cgaggcgcaa ggggcgtgaa gcggcttctg
    38101 gcagtgaggg gtgaggagtt cggaaaatcg tttttggttc tttttcgggt ttgggccaac
    38161 ggattcttac cggcttaatg cataaagtac ggtgaagttc gcgccggcgg ggtgctgttc
    38221 aattttgggg tgtaggacga aaggggttca gggagttttc atcgcagagt tttggcttta
    38281 agggggcagc aacgccggcc ctgcggacga accagatccg gagggcaaag atggggctct
    38341 tacaaagctt ccggcgggca gacgctcaag gcgaccattc tcgggacgat taccgacagc
    38401 tcacatgctg tgattccttc cgttcaggtt tcgctcacgg aaacgaccac caactttcat
    38461 cgcaccgaaa caacgaacga tagcggtttc tacgcgttca cgaaccttga ccccgggaac
    38521 taccgcgtcg aggtggaaca ccccggattc cgcaagacgg ttcgtgccgg gatcgacctc
    38581 gttcccaaca cgaccgtccg ggttgacttg gaactacagc cgggcgcggt aaccgagact
    38641 gtggatgtca cggcggaagc gccgctgcta caaaccgacc gggccgacac gggcggtaag
    38701 gtggagagca ttcaactcac cagcctgccc atgttgtgga accggaacta tcaaaacctg
    38761 ttgcaggtgg tccccggtgt gggccggtcg tatcgttcga actcgcaatt cttcgactcg
    38821 caagaccact tgcagtcggt cgtcaacggc ctggatcagc gcaataacta catgattgag
    38881 ggcgtggata ataacgtgga aaacctgacg ggcatcatcg tgcccgccga cgccatcgcc
    38941 gttgtggata ccacgaccag caattacgat ccggagctgg gccgcgccgg cggcgccgtg
    39001 gtgaacgtga tcatgaaatc gggcacaaac gatttccacg gcagcgcatt tgagtatcat
    39061 tacgataatg acatgcaggc gaagaacgtc ttcgccagca ccacgccgca cgccgtctac
    39121 aaccagtttg gcggttcgtt agggggcagg atcaagcgcg acaaattgtt cttctttgga
    39181 gattaccagg ggagccggga tcgcaaaggc aacaccaact tgcccacgat cccgacgatg
    39241 gccttccggg cgggcgatct cagcggttcg cccaccacaa tctacgatcc gctcaccggt
    39301 tctgcgagcg gcgccggccg cactccgttt cccaacaacc agattcccac ggcccgtatc
    39361 agctcaatca cgcagcaata cctgagcttt ctgcccgctc ccacgcgccc gggattctcg
    39421 gccaattttg agaagcctac gtcgacggcc aaggccattg accagtttga cgtcaaaatc
    39481 gattacgtaa tcacggccaa tgaccggatg ttcgttcgtt ataacttcca gcgcgcgctg
    39541 gtcgacgatc ccgggcttta cggccccgga ggcatttacg gcggccctca caacagcggc
    39601 tttcaaggta ccgggccctc ccggaatcag gcgccgggca tcaactactc ccacattttc
    39661 agtccgacgc tggtgaccga ggcgcgcttc ggcatcgtac gcaaccgcaa cgacgccacg
    39721 aacccggaca cagggctgcc tctgtcgcaa aagctgggca ttcccaacgc caatctcggc
    39781 gatatgtgga ccagtggatt ggccgaggtt tacgtagacg gctacgatac gccgatgata
    39841 ggggtcaatg gctgcctgcc ctggaggcgc tctgtcacga acttcgattg ggtgaacaac
    39901 tggaccaaaa caaaaggcaa ccacgtcatc aaatggggag cggacatccg ccgcgagcgg
    39961 caggatttac ttcagacgcc caccttcagc ccgcgcggcc ggtttacttt tacggccggt
    40021 cctacggcca atcccagcga tccgaaaacc agttttggca actcgttcgc tgcgtttctc
    40081 ctcgatcagc ccaacggcgt gggccgggat ctcgctttca tcttccccgc ccgccggaac
    40141 accatttaca acctctactt ccaggacaag tggcaggtta cgcagaagct gaccttggat
    40201 ctgggcctgc ggtgggagtt ctggccctcc agcacgccgc attttcctgc cggcttctcc
    40261 aattacaatc cctttaacaa cacacttgag ttggccggaa tcggcagcat tcccaacaac
    40321 ctgggtattc aaaatcaacc caagagcttc gcgccgcgat ttggcgccgc gtaccggcta
    40381 aatgacaaga cggtcttccg cggcggttac ggcatcagct atctcccgcg cacgacgaac
    40441 gtctacaact ttcctgtgaa ccagaaccag cagcttaccg ctccgaactc cttcagcgcc
    40501 gccggttcca tggcgcaagg atttcctgct ccgattgccg tggccatccc gtccacgggc
    40561 gtgatcacaa acccgccgaa ttctacgttt acctatgtag ccaaagaccg ataccagact
    40621 tatgtcgagt cctggaacgt cgccatacaa cggtcgttgc ccaggaactt tgccgtcgat
    40681 ctggcctatg tgggcaacca cacggtgaac aacggaatca cctggaacat taacgcgggc
    40741 cgcgtgcccg gggccggtcc ggccggccag ccgttgaatc agcttttcgg ccgcacggcc
    40801 gaaaccgatt acagcctgcc cacgcacgcc ttctatgacg cttttcaact gaaaatcaac
    40861 cgcaggtttt cgggcggcct gttgctcaca accgcgtacg cctatggcaa ggcgatcgat
    40921 attcaatcga acttcaacaa catcaacccg ttgtcacaaa aaggccccac ttccttcgac
    40981 cgcaggcatg tgttcacgca aagcttcctc tatgatctgc cgttcgggcc cggtaaacgc
    41041 tgggcgcact ccggtttggc caagtggatc attggcggct ggcaggtgaa cggcctggtg
    41101 acggccgaaa gcgggtttcc gttcagcatc acgttcagca gcgccacctt gaatgctccc
    41161 ggcaacagca accggccgaa cgtgagcggg ccggtcgcgg ttctcggcaa tattggccca
    41221 gggcagttgt ttttcgatac gagcaaattc agcgcccccg ctccgggaac gttcggcaac
    41281 acgccgcgaa actttcttta tgggccaaag ctgttcagca tcgacagttc tctggttcga
    41341 aagtttcgga taacggagcg cattacaggg gagctccagg gccaggcgat aaatctgacg
    41401 aacacgccgc actttgacaa cccgggggct tccttcggca gttcgtcgtt cgggcaagtg
    41461 accacggcgc agggcaacca gaccgtgaag gtaaacgaga accggcagtt ggaagtcagt
    41521 ctgagatttg ttttctgaag ctggagtgtt ttgggccgtt ctgctgctcc tttcggccgg
    41581 ttttgtttca aacagggccg gcggctggat gctgccggcc tcggcgacga tctgtggctt
    41641 gcggcgacgc gttgggcaaa tgacggggat ccctagaacg gcgggagccg gcggctatcg
    41701 ctggcatccc gggcagaaat aggtggaccg cccgctctgg cggatgcggc ggaccgtgcg
    41761 gccgcatcgt cggcaggact ctccctcgcg gccataaacc gccggatgaa agctctcgcc
    41821 ctcggcgaag cccccgggct ttcggtacgc ccgttcggcc gagcgcaccg cttcggctag
    41881 cgcctgtgcg atggcatcac gaagccggcc gatccgggat gagctcaggc tggctgcctt
    41941 ccggcgagga tcgatgccgg cttgaaacag agcctccgcc gcgtaaatat tgcccagacc
    42001 ggcaatgcgc ttctgatcca tcagcaacgg tttgacggcg cctcggctgg agcgcgcaag
    42061 cgcagcgaga acgtctgctg tgaagctttt tgacagcgga tcaacgccga tgcctgaaag
    42121 cctgttttcg atctcatcgc gctttgcggc tcgcattaca ccgagcccac ggggatcgtc
    42181 aaataccaag gcgcggccgt tatccaggag aaagtgcgcg gatgcgctcg cgggagggag
    42241 ctgccggccc gggaccgggt agagactgcc cgtcatgcgc agatgaatcc acaaactgat
    42301 cccgtcgaag tgcagcagga tattcttgcc gcggcgctct gaggagacga accggcggcc
    42361 ggctactgcc cgggtgaact cttcgggcgt ttgcggtgcg caaagccggc gccgcgaaat
    42421 gtgcgcggaa acgatgcgcc gcccgcgcag agcgcgctcg gcgatccggc gcaccgcctc
    42481 tacctcgggc agttccggca tcgcgcgaat cagcggctga gaattacggc cgcggcgccg
    42541 aagattccgg cgtcgttctc aagctgggcg ggaacaatgg gagtctgagc ggcaaacgga
    42601 ttgacgctcc gttggggaac ggtttgacgg agtttcgaga acagcggctc gccgattttg
    42661 gtcactccgc cgccgatcac cacgatatcg gggtcgagca gattcacgat
//
LOCUS       DASREK010000279        12762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_805153, whole genome shotgun sequence.
ACCESSION   DASREK010000279 DASREK010000000
VERSION     DASREK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805153"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFA54_15160"
     CDS             complement(<1..237)
                     /locus_tag="VFA54_15160"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HZS52205.1"
                     /translation="MATPSDDELLELMIAGDEDAFLTLYRRRQGPIYRFALHMSGSAS
                     IAEEVTQEVFLALLRDGRQYNPVRGSLAAYMYGVA"
     gene            complement(341..1210)
                     /locus_tag="VFA54_15165"
     CDS             complement(341..1210)
                     /locus_tag="VFA54_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS52206.1"
                     /translation="MKGVYDLTIVRLKLLVRNRSAIFFSIIFPILFLFVYGFLFAKGD
                     SEKVRFLMGPVVVLTILGSGFWGVGFQIVFFREKNILRRFRPAPVGAGPLLISGILAN
                     WLLQIFVVSLEFLIARWIFHIPWPLQPLELFIIATLGLFAFAGIGMVIASIANTMQET
                     QVLCNLLWFAFMFLSGATAPLAQLPEVIRHIALFLPSFYLVYAMQAIMFGGASLSAML
                     PEMTALAMTTVAAYLLGLKLFRWDQHMKLSWSAKAWAVPAVIPFLALGVWANLFSHRI
                     DQALVLYGHVWPH"
     gene            complement(1207..2130)
                     /locus_tag="VFA54_15170"
     CDS             complement(1207..2130)
                     /locus_tag="VFA54_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS52207.1"
                     /translation="MPGEPIVEVRDLRKNYAALEAVRGLSFEIERGEVFGLLGPNGAG
                     KTTTIEILEGLRSRDGGKVVVCGFDPGLHARQLKEKIGIQLQQTALPDKITVEEALNL
                     FRSFYQRSLRTEQLLEWFGLQEKRKTHYDLLSGGQQQRLALALAVVNDPEMVMFDEPT
                     AGLDPQTRREIYSLIGRLRDERRTVLLTTHYIEEAEQLCDRVAIVDRGKIIASGTPRG
                     LIEQSRQCSRIEFIPRSPVDLTRLHALDCAEDVTPSDGGYRIRSREAPRTLMALMRML
                     IAENNEVIQVHLERPSLEDVFIELTGRSLRD"
     gene            2341..3288
                     /gene="ftsY"
                     /locus_tag="VFA54_15175"
     CDS             2341..3288
                     /gene="ftsY"
                     /locus_tag="VFA54_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZS52208.1"
                     /translation="MIQTLFGSAPPEPGLFERLQKAVEKTRAGLVNRIEDLVQGKKEI
                     DSDLLGELEAALISADLGVRTATEVLDAVRTKVERRQLHNADELKSHIRQQLLDILKS
                     ADRPATVISGEPEVILVVGVNGTGKTTTIGKLASLLRNQGKNVLLCAADTFRAAAIEQ
                     LEIWGQRTSTEVIKQKAGADPSAVLFDAAQAARARKMDYLIVDTAGRLHTKSNLMAEL
                     EKMRRTAGRVIPGAPHEVLLVLDATTGQNGLEQARQFTNCAGVTGIVLAKLDGTAKGG
                     VVIAIARELGLPIRYVGVGEKADDLIRFDPESFIQSLFE"
     gene            complement(3302..5317)
                     /locus_tag="VFA54_15180"
     CDS             complement(3302..5317)
                     /locus_tag="VFA54_15180"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HZS52209.1"
                     /translation="MLLYAAAIFLGAFLLFLIEPMIAKAILPWFGGAAAVWTTCLLFF
                     QGMLLGGYLYSDRLASVRSKPRNQALWHLALLWASVFLLPISPSQRWKPPAADDPTFR
                     ILGLLLATIGLPYLLLSTGPLLQAWYARQQPAAAPYRLFAVSNAGSLLGLLSYPILLE
                     PFFTTRRQFQCWSVLYSGFVLLVTAAGVRYVRARNFEAASRAAGTAPPSLDVHLFWIA
                     FPACSSLLLLAVTNHLSRNVAPIPFLWVLPLALYLLSFILCFEREAFYKRGWYLRLLL
                     VAFAAMVCALSGLGEAVGLPALVAIFAGGLFVCCIVCHGELARLKPAAEHLTRYYILV
                     AFGGAVGGAFVALIAPRIFCDNTELPAGLVLCLLLPSLILLRSTPLQRVLLVSIGVAL
                     AGVLYALLYSGAFRPESRLVARNFYGVLRVQDHLGSDPSRATRELRSGTINHGQQFLA
                     PERRRRPTAYYGPTSGVGLALLHCRRDLPQRVGVIGLGAGVLASYARAGDYYRFYEIN
                     PLVVRLAHTHFTFLRDSPAPIDVVLGDGRLCLEREAAQNFDILAVDAFSGDAIPVHLL
                     TREAFLVYFRHIKQNGILAVHISNRYLDLAPIVQLAVQAFGRRAIVIENRPDPEAAIL
                     RSTWVLAALQLEDLPQPAIRDVESHRPIPAGTRPWTDDYSNIFSILR"
     gene            5454..6563
                     /gene="ribD"
                     /locus_tag="VFA54_15185"
     CDS             5454..6563
                     /gene="ribD"
                     /locus_tag="VFA54_15185"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZS52210.1"
                     /translation="MGVTAEEKRYLEEALQLAERGRALASPNPMVGAVLVNNGAVVGR
                     GFHTYERKKHAEIIALEEAGARARGATLYINLEPCSHDGRTPPCADALIAAGVKRVVA
                     PIEDPNPLVAGAGFARLKAAGIVVELAPEFEQQALHLNEAFIHFIRSGRPLVTLKAAL
                     TLDGKISAPDDNTGWITSERARAHVQQVRHDHDAILTGIGTVLSDDCLLTDRTGLPRR
                     LPLLRIVVDSQLRLPAGSRIVQSFQQDLVVAATSAASPARRQALSDLGIPILTLDGPD
                     GRVDLRALVRWLGGQKKISLMIEAGAKLNWAALEADIVDKVLFYYAPKILGGMESLPL
                     AAGPGRRSRSSAIKLHSLRTFAVAADEFAVEAYVH"
     gene            complement(6551..7138)
                     /locus_tag="VFA54_15190"
     CDS             complement(6551..7138)
                     /locus_tag="VFA54_15190"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HZS52211.1"
                     /translation="MAFPATCRLALKEWAVTQQALDRAEQILLLRKGGLREEGKSFDV
                     LQPEFLIYPTYEHQREDLLKTVYRERLSATLAHPHRPDRIVFSHWARVAERIELRETK
                     QLEALSPYYIFTTDYAEARLHWKPRSPLQLLLVRVYRLSKPRSLPYRAAYGGCKSWVA
                     LEEAVELGALAPVLNEPQFQARVREIRRALGLQCT"
     gene            7262..9091
                     /locus_tag="VFA54_15195"
     CDS             7262..9091
                     /locus_tag="VFA54_15195"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZS52212.1"
                     /translation="MANLSKPLLVFDGNCGFCRLWIDRWKHLTADRVDYAPFQEAAGR
                     FPNIPLEQFRSAVQLIEPSGAVTSGAQAVFRLLAYGQGKRWLNWLYDHAPGFAPVSEA
                     CYRFVAQHRPLLYRLSRLLWGSQFERPSNALTRRLFLRILAIIYVIAFLSLGVQITGL
                     VGSQGVLPASHLLDAVRSGAAEQRYWALPTLAWLSASDGALRAMCFGGVAISAAGFFG
                     ILPPVMFAAAWALYLSLVVIGQDFLSFQWDILLLETGFLAIFFAPWQLRAGSIRREPS
                     RAILWLLRILLFRLMFSSGAVKLTSGDPTWRSLRALDYHYQTQPLPTPLAWLMQQLPH
                     GFQTLSVLVMFGIELVAPFFLFAPRRVRLWAAGLIAFLQALILLTGNYAFFNLLALAL
                     CLVCLDDAFLHRFFPRRAPPERILREPRLKRAIVAVLFVVILFAGAEQAAGVLGFWGS
                     LPRAAMQIFTGLGRFYIVNSYGLFAVMTTTRYEIIVEGSNDGQTWLPYEFKYKPGDIK
                     RRPPIVEPHQPRLDWQMWFAALGDYRDNPWFVNFMVRLLEGSPQVLALLAKNPFPGSP
                     PRYIRAELYDYRFTDFAEWRATGAWWRREFVRLYFPVASLRSR"
     gene            complement(9155..10286)
                     /gene="prfB"
                     /locus_tag="VFA54_15200"
     CDS             complement(join(9155..10213,10215..10286))
                     /gene="prfB"
                     /locus_tag="VFA54_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_106861312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZS52213.1"
                     /translation="MMIDELERGFTALRDKVHDIRSYLDLPQKQAQLAEIEKRIADPA
                     FWSDPEKSQKVMQERKRLEQAVSTDQDLAGRLSDLEALFELAREGEPVEDDIRREMDA
                     LGATAERLETNMLLSGENDARNAIVTLHPGAGGVESQDWAQMLMRMYLRWAEREGFNV
                     TINDVQPGEEAGIKSATFTVAGENAYGLLQSETGVHRLVRISPFDSNARRHTSFASVF
                     VYPEIDDEITVQINPDDLRVDTFCSSGPGGQGVNTTYSAVRLTHLPTGIVVQCQNERS
                     QIKNKAFAMKILRSRLYELELERKREETKKLEASKMGIQFGSQIRSYVLAPYRLVKDH
                     RTKFEMGDVDRVLDGDLEPFIHAYLVWKKTGAPVAEAAPERE"
     gene            complement(10305..11789)
                     /gene="lnt"
                     /locus_tag="VFA54_15205"
     CDS             complement(10305..11789)
                     /gene="lnt"
                     /locus_tag="VFA54_15205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZS52214.1"
                     /translation="MAAPWKRVALAAATGVLLVLLFPNAGLSYLAPVALVPLLLALNS
                     ESAWGRRFLLGWLSGAIFWGGACYWIYYVMHVHGHVPAIGAAALLAGFMVVKGLHLGV
                     FGVIAGPQLRRAWAIPAVGAAWVAIEGTHQYAGFTWLMLGNAATNMAVVARLAPVTGV
                     AGMSFVLAMMNVAVLLAIERRPRRHLAWLGVLPLLYFLPPLPGSPPGRSVARLVQPNI
                     SEESIVSEPWTAERAGRLLRKMAALSSSRSPAAPSPDLIIWPENPAPFYFYNDPRFRS
                     WVEAIARAQHADLLFGTVAFRSPGLREPLNSAVLLDAEGNEVARYDKIHLVPFGEFVP
                     WPFGHLVEKVTHEAGDFVAGDKVVVARSGQHSIGTFICYESVFGPEVRKFVAGGAQVL
                     VNISNDGWFGRSAARYQHLLIARMRALENGRWLLRATNTGVTAVIDPAGRVRGSLPLD
                     QPGVLAVYFDYLSHLTLYTRWGDWFWWVAAAGTVAQAGWLLGAR"
     gene            complement(11794..>12762)
                     /gene="lipA"
                     /locus_tag="VFA54_15210"
     CDS             complement(11794..>12762)
                     /gene="lipA"
                     /locus_tag="VFA54_15210"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZS52215.1"
                     /translation="ADESRSRKGWFQINSPQTIGRPALVDPILMDLVQIEMPRPRLPE
                     WARRPATFYSSLHDLKAELRKFHLHTVCESARCPNIQECFHRGAATFMILGNVCTRTC
                     GFCAVPRGRPEPLDPREPANVARMAARMRLRYVVITSVNRDELADGGSKHFAATVREV
                     KRALPTAQVEVLTPDFRGNCCAVACVLEAGPHVFNHNVETVPRLYRRVRPQADYRQSL
                     GVLRFARRHSPGMLTKSGFMVGLGERGDEIEALLADLRDSGVDVVTIGQYLQPTRRHL
                     PVEKFVAPEVFDQYRTFALELGFRAVFSGPLVRSSYMADLVAAEAG"
BASE COUNT         2597 a         3911 c         3905 g         2349 t
ORIGIN      
        1 agccacgcca tacatgtagg cggccaggga cccgcgcacc gggttgtact ggcggccgtc
       61 ccgaagcaga gccaggaaaa cctcctgggt tacctcttcc gctatgctgg cgctgccgct
      121 catgtggaga gcaaaccgat agatcggccc ttgtcgccgc cggtacaggg tgaggaatgc
      181 gtcctcatcg cccgcgatca tcaattccag caactcgtcg tcgctcgggg tcgccatggg
      241 ttgccttgcg gccggaagta agccgctatg aataattccc cgctcgaggc ggaaagttcc
      301 gaaaaattcc cggcggtttt cagcacaccc tcggggggag tcagtgcggc caaacatggc
      361 cgtacagtac caatgcctgg tcgatcctat gagagaacag gttggcccaa acacccagag
      421 caagaaaagg aataacggcg ggaacggccc aggccttggc gctccaggag agtttcatgt
      481 gttgatccca gcggaacagc ttcaggccca acaggtaagc ggccacggtg gtcatagcca
      541 gagccgtcat ctcgggcaac atggctgaga gactggcccc gccgaacatg atcgcttgca
      601 tggcgtatac aagataaaaa ctcggtaaaa acagcgcgat atgccgaatg acctccggga
      661 gctgggcgag cggcgccgtc gctccggaga gaaacataaa ggcaaaccag agcaggttgc
      721 agagcacttg cgtttcctgc atagtgttag cgatgctggc gattaccatg ccgatgccgg
      781 cgaatgcaaa cagtcccagc gtggcgatga taaaaagttc cagcggctgt aagggccacg
      841 gaatgtggaa tatccagcgg gcgatcaaaa actccaggct gacgacaaag atctgtagca
      901 gccagttggc cagtatcccg ctgatgagca gcggccccgc ccccaccggc gccggccgaa
      961 agcgccgcaa gatattcttt tcgcgaaaaa agacaatttg aaagcccacg ccccagaaac
     1021 cgcttccaag aatcgtcagc actaccaccg gacccatcag gaagcgcacc ttctccgaat
     1081 ctcccttggc gaagaggaaa ccatagacga acaaaaacag gatcgggaaa atgatcgaaa
     1141 agaaaatggc gctgcggttg cgaaccagca gcttgagacg cacaatggtg aggtcgtaga
     1201 cgcctttcaa tcgcgcaggc tccttcctgt cagctcgatg aacacatcct cgagcgaagg
     1261 cctttccagg tggacctgga tgacctcatt gttttccgcg atcaacattc gcatcagggc
     1321 cataagagtg cgcggcgcct cgcggctgcg gatcctgtac ccgccgtcgc tcggcgtcac
     1381 gtcttcggcg cagtccaggg catggagacg cgtcaagtca accggggagc gagggatgaa
     1441 ctcgatgcgg ctgcactgcc gcgattgctc gatgagcccg cggggcgtgc cggaagcaat
     1501 gatcttgccc cggtccacga tcgccacgcg gtcgcaaagc tgctcggcct cctcgatgta
     1561 atgggtcgtg aggagcacag tgcggcgttc gtcccggagc cgcccgatca gactataaat
     1621 ctcccgccgg gtctgcgggt cgagcccggc cgttggttcg tcaaacatca ccatctccgg
     1681 atcgttgacg acggccaggg ccaaggccag ccgctgctgc tggcctccgg aaagcagatc
     1741 gtaatgggtc ttacgctttt cctgaaggcc gaaccactcc agcagttgct ccgtgcgcag
     1801 gctcctctgg tagaacgagc ggaacaaatt gagcgcctcc tcgaccgtga tcttgtccgg
     1861 cagcgcggtt tgctgcaatt gaatgccgat cttttccttt agttgccggg catgcaaacc
     1921 cggatcgaag ccgcacacca caacctttcc gccatcccgc gagcggagac cttcgagaat
     1981 ttcgatggtg gtcgttttcc cggctccatt cgggccaagc aagccgaaca cctcgcctcg
     2041 ctctatctcg aagctcagtc ctcgaaccgc ctccagcgcc gcgtagttct tccgcagatc
     2101 gcgcacttcg acaatgggtt cgccaggcat gttgggccgg atgctattat gggttgagca
     2161 gcctgttctc agcttttcca aacggttcca acgctcccgc gcgggcgact cccctgcgcg
     2221 gcggagtgga cgagaacagc cgccggccag ccgcacggcc ggaagccggc gcttgtggaa
     2281 atcctcgccg cagacgggaa ccgcgcaagc tagcgctggg aactgaggcc gccagttatc
     2341 atgatacaaa ccctgttcgg ctccgcgcca cctgaacccg gcctgttcga gcgcctgcaa
     2401 aaggcggtgg agaagacccg cgctggcctc gtgaatcgaa tcgaggatct ggtccagggc
     2461 aagaaggaaa ttgactctga cctcctcggc gagctcgagg ccgcgctgat ttcagccgac
     2521 ctcggtgtgc ggaccgccac ggaagttctg gatgcagtcc ggaccaaagt cgagcgccgg
     2581 caattgcata acgccgatga actcaagtcc cacattcggc agcaactgct ggacatcctc
     2641 aagagcgcgg atcgtccggc tacggtgatc tccggcgagc cggaagtgat cctggtggtc
     2701 ggggtcaacg gtaccggaaa aactacaact ataggaaagc tcgccagtct gctgcgaaat
     2761 cagggtaaaa atgttctgct gtgtgccgcg gacacctttc gggcggcagc catcgaacag
     2821 ctcgagatct ggggccagcg cacctcgacg gaagtgatca aacagaaagc cggcgctgac
     2881 ccgagcgctg ttctcttcga tgcggcgcag gcggcgcggg ccagaaaaat ggactacctc
     2941 attgtggata ccgccggccg cctccacaca aagtcgaatt tgatggccga actggagaaa
     3001 atgcggcgga ccgcaggccg ggttattcca ggggcgcccc acgaagtgct cctcgtgctg
     3061 gatgcgacta ccggccagaa cggcctggag caggcccggc agttcaccaa ctgcgctggc
     3121 gtgaccggca tcgttctagc caagcttgat gggaccgcaa agggcggcgt ggtcatcgcc
     3181 atcgcccgag aactcggctt acccatacgc tatgtgggag tcggtgaaaa ggcggacgac
     3241 ctgattcgtt tcgatccgga gagcttcatc caatctctct tcgagtaacg atttcataaa
     3301 atcatcgtaa tatactgaaa atgttgctgt aatcgtctgt ccaaggacgt gttccggcgg
     3361 ggattgggcg gtgtgattcc acgtcgcgga tggctggctg tggaagatct tcgagctgca
     3421 aggcggccag cacccacgtg gagcgcagaa tagcggcctc gggatccggc cggttctcga
     3481 tcacgatcgc ccgcctgccg aacgcctgaa ccgcgagttg cacaataggg gctaagtcta
     3541 agtaacgatt ggatatatga actgccagga tgccgttttg ttttatgtgc cggaaataga
     3601 cgagaaaggc ttcccgggtg agcaggtgga cgggaatcgc gtctcccgaa aatgcatcca
     3661 ctgccagtat gtcgaagttt tgagctgcct cgcgctcgag gcataggcgc ccgtctccca
     3721 gaactacgtc aattggggct ggcgagtccc gcagaaacgt gaagtgcgtg tgggcgagtc
     3781 tcaccaccaa aggattgatt tcgtaaaagc ggtagtagtc ccctgcccgc gcatagctgg
     3841 ctagcacccc ggcgccgagg ccaatcacgc ccactcgctg tgggagatcg cggcggcaat
     3901 gtagcaacgc aagcccaacg ccggaagtgg ggccatagta tgcagtgggc cggcggcgcc
     3961 tctcaggggc gaggaactgc tggccatggt tgatcgtgcc ggacctcagc tcacgggtag
     4021 ctctggatgg gtcgctgccc aggtgatctt gtacccggag cacgccgtag aaattgcgcg
     4081 ccactagacg cgactcgggc ctgaaggctc cgctgtacag cagggcgtaa agcacgcccg
     4141 ccagcgccac gccgatagat accaacagaa cgcgctggag cggcgtggat cggagcaaga
     4201 tgagcgaggg caacaacagg caaagcacca gtccggcagg cagctccgtg ttgtcgcaga
     4261 aaatgcgggg cgcaatcagc gcaacaaagg cgccgccgac ggccccaccg aaggccacca
     4321 ggatgtagta gcgggtgaga tgctcggccg ccggtttcag cctggccagt tctccgtggc
     4381 aaacaataca acacacaaat agccccccgg cgaagattgc caccagagcc gggagcccga
     4441 ccgcctctcc aaggccggag agcgcgcaga ccatcgcggc gaatgccacc aggagcaagc
     4501 gcagatacca gccccgtttg taaaaggctt cccgctcgaa acacaggatg aagctcaaaa
     4561 ggtacagcgc cagcggcaga acccatagaa agggaatcgg ggcgacgttc ctggaaagat
     4621 gattcgtgac cgccaggagc aacaacgaag aacaggcggg aaatgcaatc cagaaaaggt
     4681 gaacgtccaa cgacggcggc gcagttcccg ccgctctgga agccgcctcg aagtttcgtg
     4741 cacgcacata ccgcacgccg gcggctgtga ccaggagtac gaaaccggag tacaaaacag
     4801 accagcactg gaactggcgg cgagtggtaa aaaacggctc gagcagaatc gggtagctca
     4861 acagccctag cagggagcct gcgttcgaaa ctgcaaacaa tcggtacgga gctgcggcgg
     4921 gctgctgccg ggcgtaccag gcctgcaaca gcggaccggt ggagagcaga agatacggca
     4981 ggccgatcgt ggcaagcagc aggcccaaaa tccggaaggt cggatcatcg gccgcaggtg
     5041 gtttccaccg ttgactcgga gaaatgggga gcaagaagac gcttgcccag agcagggcca
     5101 ggtgccaaag ggcttggttg cgcggtttcg agcggacaga agccagccgg tcggaataga
     5161 gatacccgcc gagcagcatc ccttgaaaaa acagcaggca agtggtccaa acggcggctg
     5221 cgccgccgaa ccagggaagg atcgccttgg cgatcatggg ctcgatcagg aagagcagaa
     5281 aggccccgag aaaaattgcg gccgcgtaaa gcagcatgga agctggccat cttagcacgc
     5341 agcatccgcc gctcatcacc gcggctggcg cggcgccccg cacccgcccc tttttgcgcg
     5401 cgctcgcgcc ggcgccaggg tggcgaacgg gctacggccg attatgctga aatatgggcg
     5461 tgacggcgga agaaaagcgc tatctggagg aagcgctcca actggccgaa cggggccggg
     5521 cgctggcctc acccaatcct atggtagggg cggtgctggt caacaacggg gctgttgttg
     5581 gacgcggctt tcacacctac gagaggaaga agcacgcgga gatcatagcg cttgaagaag
     5641 cgggcgcgcg ggccagaggc gccacgttgt acatcaacct cgagccgtgt tcccacgacg
     5701 gccgcacacc gccctgcgcc gacgcattga ttgctgctgg agtgaagagg gtggtggctc
     5761 ccatcgagga tccgaatccc ctcgttgcgg gcgcaggttt cgcgcggcta aaggcggcgg
     5821 ggattgtcgt ggagctggcg cccgaatttg aacagcaggc gctgcatctc aatgaagcct
     5881 tcatccattt cattcgcagc ggccgccctt tggttacgct gaaggcggcg ctgacgctcg
     5941 acggaaaaat ttccgcgccc gatgacaaca cggggtggat caccagcgag cgggcgcgcg
     6001 cgcacgtgca gcaggtccgc cacgaccacg acgccatcct caccggcatc ggaactgttc
     6061 tttccgatga ttgcctgctt accgaccgga cggggctgcc gcgccggctg ccgctgttgc
     6121 ggatagtggt cgattcccag ttgcggctgc cggccggctc gcgcatcgtc caatcatttc
     6181 agcaggacct ggtggtcgct gccacttcgg cggcttcccc cgcgcgccgg caggctctca
     6241 gtgatctggg catccccatt ctgactctcg atggtccgga cgggcgcgtg gatcttcgcg
     6301 ctttggtgcg atggctcggc gggcagaaaa agatttcctt gatgatcgag gcgggagcga
     6361 aactgaattg ggcggccctc gaggcggaca ttgtggacaa agttctgttt tattatgctc
     6421 ccaaaattct gggcggcatg gagtcccttc ccttggcggc aggcccggga cgccgcagcc
     6481 ggtcctcggc gatcaagctg cacagtctgc gcacgttcgc cgttgcggcc gacgagtttg
     6541 cagtcgaggc ctacgtacac tgaagaccga gggcgcgccg gatctcccgg accctggcct
     6601 gaaactgcgg ctcattcagg accggcgcca aagcgccaag ctccaccgcc tcttcgagcg
     6661 cgacccagga tttgcagccg ccataggcag ctctataggg aaggctccgc ggcttgctca
     6721 ggcgatagac ccggaccagg agcaattgca gggggctgcg cggcttccaa tgcaggcggg
     6781 cctcggcata gtcggtcgta aaaatgtaat agggcgagag ggcctcgagt tgtttggttt
     6841 ctctaagctc gatgcgctct gcgacgcgcg cccaatgaga aaacacgatt cgatccggtc
     6901 gatgcgggtg ggccagggtg gcgctcaggc gctcgcgata gacagttttc aggagatctt
     6961 cgcgctggtg ttcataggtg ggatagatga ggaactccgg ctggaggacg tcgaacgact
     7021 tcccttcttc gcggaggcca cctttgcgca gaagcagaat ttgctccgcg cggtccagcg
     7081 cctgctgggt cactgcccat tccttgagcg ccagccggca agtcgcagga aacgccattg
     7141 ctgttcccat tatcgcctgc aatccacagg ctgctctcaa cacgccgccg ggcaatgatt
     7201 catcccggcc gctgctacgg gctggctaag atagtggaga cgccggcggc tgccaagacc
     7261 cgtggctaac ctgtccaagc ccctgctcgt tttcgacggc aattgcggtt tctgccgctt
     7321 gtggatcgac cggtggaaac atcttacagc cgatcgggtt gattacgcgc cgtttcagga
     7381 ggccgccggc cgctttccca atattccgct ggaacagttc cgcagcgccg tgcagcttat
     7441 cgagccttcg ggcgcagtca caagcggcgc acaggcggta ttccggctgc ttgcgtacgg
     7501 gcaaggcaaa aggtggctga attggttgta tgaccacgct ccggggtttg cgcctgtctc
     7561 cgaagcctgt taccggttcg tagcacagca ccgcccgctc ctttaccgcc tgtcccgctt
     7621 gctttgggga tcgcaattcg agcgcccttc caacgcactg acgcgccggc tgttcctgcg
     7681 aatcctagcg atcatttacg taatcgcttt cctgtcgctc ggcgttcaga tcacgggctt
     7741 ggtcggcagc cagggcgtcc tgcccgcttc gcacctgctg gatgctgttc ggagcggcgc
     7801 agccgagcag cgctattggg cgctccccac cttggcctgg ctcagcgcga gcgacggcgc
     7861 tttgcgagcc atgtgtttcg gcggcgttgc catatcagcg gcaggcttct tcggaatcct
     7921 gcctcctgtg atgttcgccg ccgcctgggc cctttactta tctctcgtag tgatcggcca
     7981 ggacttcctg tcgtttcaat gggacattct gctccttgag acaggttttc tggccatttt
     8041 cttcgctccg tggcagcttc gcgccggtag tatccgcaga gaaccttccc gcgccattct
     8101 gtggctgctg cggattctgc tttttcggct gatgttttct tccggcgcag tcaaactgac
     8161 cagcggcgac ccgacttggc ggagcctacg cgctttggat tatcactacc agacccaacc
     8221 gctgcctacc ccgctcgcct ggcttatgca gcaactgccg cacggctttc agactttatc
     8281 agtgctcgtt atgttcggga tcgagctggt cgcaccgttt tttctcttcg cgccgcgccg
     8341 ggtgcggctt tgggcggccg gcctgattgc atttctgcaa gcgctgattc tgcttaccgg
     8401 caattacgcc tttttcaatt tgcttgcgct ggcgctgtgc ctggtctgtc tggacgacgc
     8461 ttttctgcac cgcttttttc cccgccgcgc tccgcccgag cggatactga gggaaccgcg
     8521 cttgaagcgc gctatcgtcg cagtcctgtt cgttgtgatt ctatttgctg gagcggagca
     8581 ggcggcggga gtactcggtt tctggggaag cctgccacgg gcggccatgc agattttcac
     8641 cggcttgggg cggttctata tcgtgaacag ctacggcctg ttcgctgtga tgaccaccac
     8701 tcgatacgag atcatcgtgg agggaagcaa tgacggccag acctggctgc cctacgagtt
     8761 caaatacaaa ccaggagaca taaagaggcg gccgccgatt gtcgagcccc accagccaag
     8821 gctcgactgg cagatgtggt tcgccgcgct gggcgattac cgcgacaacc cgtggttcgt
     8881 aaattttatg gtgcggctgc tcgagggatc tccgcaggtg ctggcgctgc tggcgaaaaa
     8941 tccgttcccc ggctctcccc cgcgctacat ccgcgccgag ctttatgact accggtttac
     9001 tgattttgca gaatggcgag caacaggcgc ctggtggcgc cgggagttcg tgcggctgta
     9061 tttccccgtg gcatcccttc gatccaggta gcgcggaacc cgttttgggg actcgatcgg
     9121 gcggccgggt gtaccgcagc gcccgcgaat cagattactc ccgttccggc gcggcttcgg
     9181 ctaccggggc gcccgtcttc ttccacacca gatacgcgtg gatgaaaggc tccagatcgc
     9241 catccaggac ccgatccaca tcccccattt cgaactttgt ccggtggtcc ttcaccagcc
     9301 ggtacggggc taatacatag cttcgaatct ggctcccaaa ctggatcccc atcttcgagg
     9361 cttcaagctt cttggtctcc tcccgctttc tctccagttc gagctcgtaa agccgcgagc
     9421 ggagaatctt catggcgaag gctttgttct tgatctggga acgctcgttc tggcactgca
     9481 caacaatacc agtcggcagg tgcgtcaggc ggaccgcgga atatgtcgta ttcacgccct
     9541 ggccgccagg gcccgacgaa caaaaggtat caacgcgcag atcgtccgga ttgatctgca
     9601 ccgtgatctc gtcatcgatc tccggataga cgaacaccga tgcgaatgat gtgtggcggc
     9661 gcgcattgga atcgaacggc gagattcgca ccaagcggtg tacgcctgtt tcggattgca
     9721 gcagcccgta ggcgttttca ccggcgacgg tgaaggtcgc cgacttgatg ccagcctctt
     9781 cccccggctg gacgtcgttg atggtcacgt tgaaaccttc ccgctcggcc cagcgcaggt
     9841 acatccgcat gagcatttgc gcccagtctt gcgattcgac gccgccggcg cccggatgca
     9901 gggtcacgat ggcattgcgg gcgtcgtttt cacccgagag cagcatgttc gtctcgaggc
     9961 gctcggctgt cgccccgagt gcatccatct cgcggcgaat atcgtcctcg accggctcgc
    10021 cttcgcgcgc cagttcgaac aaggcttcca ggtcggagag gcggccggcg aggtcctgat
    10081 ccgtagaaac ggcctgctcc agccgcttcc gctcctgcat caccttctgg cttttctcgg
    10141 ggtcgctcca gaaggccgga tccgcgatcc gcttctcaat ttcggctaat tgcgcctgtt
    10201 tctggggaag gtcaaagata gctccggatg tcgtggacct tatcccggag cgccgtaaat
    10261 ccgcgttcta gttcgtcaat catgattgcc tcaatccggc cgtgttatcg tgcgccgagc
    10321 agccatcccg cctgggccac agtgccggca gccgccaccc accagaacca gtctccccag
    10381 cgcgtataaa gggtcaagtg gctcaggtaa tcgaagtaga cagccaaaac acccggctga
    10441 tcgagcggca ggctgccgcg cacgcgccct gccgggtcga tgaccgccgt aactccggtg
    10501 ttggtggcgc gaagcagcca tcgtccgttt tcaagcgccc tcatccgggc tatcagcagg
    10561 tgctgatagc gcgcggctga ccggccaaac cagccatcat tcgatatatt aaccaggacc
    10621 tgggcgccgc cagcaacaaa cttcctgacc tccgggccga agacggactc atagcaaatg
    10681 aaagtgccga tgctgtgctg gcccgaccgg gccacaacaa ccttgtcccc ggcaacgaaa
    10741 tcgccggcct cgtgagtgac cttttcaaca agatggccga acggccaagg aacaaactcg
    10801 ccgaagggta ccaaatgaat tttatcatac cgggccactt cgttgccctc cgcgtcgaga
    10861 agcacggcgg aattgagcgg ctcgcgcaag cccgggctgc ggaaggcgac cgtgccgaac
    10921 agcaggtccg cgtgctgggc tcgggcgatg gcttccaccc aagaacggaa ccgcggatcg
    10981 ttgtagaaat aaaaaggcgc gggattttct ggccagatga tcagatcggg acttggcgct
    11041 gctggcgaac gggacgaaga cagcgccgcc attttccgca gcagccgccc ggcgcgctcg
    11101 gcagtccatg gctccgagac aatgctttcc tcggagatat tcggctggac caggcgcgcc
    11161 acactgcgcc caggcggaga cccgggcagc ggcggcagaa aatagagcag cggcagcaca
    11221 cccagccacg cgaggtgcct gcgcgggcgc cgctcgatgg ccagcagtac cgccacgttc
    11281 atcatcgcga ggacaaacga catgccggcg acgccagtga ccggcgcaag gcgggccacc
    11341 actgccatgt tcgtggcggc gttgccgagc atcagccagg taaagccggc gtactggtgt
    11401 gtgccctcga tcgccaccca ggcggctccg acggccggaa tcgcccatgc gcggcgcaac
    11461 tgcggtccgg caatgacgcc gaagacgcct aggtgcaaac ccttaaccac catgaaaccg
    11521 gccagcaaag cggccgctcc gatggcaggc acgtgtccgt gcacgtgcat tacgtagtag
    11581 atccagtagc acgcgccgcc ccagaagatt gcgcccgaga gccaacccag tagaaatcgc
    11641 cgcccccacg cagattccga atttaaagcc agcagcaacg gaaccagggc gacgggagcc
    11701 aaataggaaa ggccggcgtt gggaaacaac aggacgagca acacaccggt ggccgccgcc
    11761 agggcaaccc gcttccatgg ggcagccacg gggttatcca gcctccgcgg cgaccagatc
    11821 ggccatgtag gagctgcgca ccagcgggcc gctaaaaacc gcgcgaaagc caagttctaa
    11881 ggcgaaagtg cggtattgat cgaaaacctc cggcgcgacg aatttttcga ctggcagatg
    11941 acgccgggtg ggttggagat actgtccgat ggtaacgacg tcaacgccgg aatcccgcaa
    12001 atcggcgagc aaggcctcaa tttcgtcccc gcgctcgccc aggccgacca taaagcctga
    12061 tttcgttagc atgccggggg agtgccgccg cgcgaatcgg agcacaccca acgattgccg
    12121 gtaatcggcc tgcgggcgca cgcggcggta gagccgcggc acggtttcga cattgtgatt
    12181 gaaaacgtgg ggtcctgctt ccagcacgca agccacagcg cagcaattgc ctcgaaaatc
    12241 cggggtgagc acttctacct gggcggtggg cagcgcgcgt ttcacttctc tgacagtcgc
    12301 ggcgaagtgc ttcgagccgc catcggccag ctcgtcgcga ttcacgcttg tgatcactac
    12361 gtaacgcagc cgcattcgag cggccatgcg ggccacgttg gccggctccc ggggatccag
    12421 aggctccggc cggccccggg gcacagcgca aaacccgcac gtgcgggtgc acacgttccc
    12481 caggatcatg aatgtggccg ctccacggtg aaagcattcc tggatattcg ggcagcgcgc
    12541 cgattcacaa acggtgtgca ggtgaaactt gcggagctct gccttcagat cgtgcagtga
    12601 cgagtagaaa gtcgccggcc ggcgcgccca ttcgggcaac ctcgggcgcg gcatttcgat
    12661 ttgaaccagg tccatgagaa tcgggtccac cagggcgggc cggcctatgg tctgcggaga
    12721 attgatttga aaccatcctt ttcgagagcg gctttcatct gc
//
LOCUS       DASREK010000280         7322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2283890, whole genome shotgun sequence.
ACCESSION   DASREK010000280 DASREK010000000
VERSION     DASREK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283890"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VFA54_15215"
     CDS             <1..666
                     /locus_tag="VFA54_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZS52216.1"
                     /translation="REAIEHFHLSPARVAAVPLAPSRQFQPRGDEETSRLLRRLSIAR
                     PYLLFVGTQEPRKNLGRLIEAWREVRREHPDLGLVLAGRPGDNFSLPEVLNVAGEPGL
                     TITGRLADADIAGLMSRAALFVYPSLYEGFGLPVLEAMQAGAPVVISRDPALREVAGD
                     AAVSVDAGSPVSLACAIVQLMKDPRERMELRARGRRRAAQFCWRYTAIRTHEIYAEAL
                     QRF"
     gene            641..1897
                     /locus_tag="VFA54_15220"
     CDS             641..1897
                     /locus_tag="VFA54_15220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52217.1"
                     /translation="MQKLSSGSEWALLLAPESPYPMTSGGAVRTASICLFLSARFRLH
                     LVGFKVGNGMEAAPALPAGAAERVDWISLPNHSRRMPARLARNVSRLSRSVLPLTDRF
                     SGAESMGRVARTLAGRSYRIAVLEHFWSAHYEELVRQHAGLIVMDMHNVESAFHARCA
                     ETEAWPERWAHRRFAALAAREESRWLPRFDLVLAASEADRQRILGLAPGARVAVYPNA
                     VPARFPEECAEEHCIAFSGNLAYHPNTAAVRYFAREIWPVLRRRDPALRWRLIGSNPQ
                     AVQRWIAGNPSIEATGPVEDALAELARIRLAVVPLRVGSGTRVKILEAWAAGRAVVST
                     RIGAEGLPAVHGENLLLADSPADMVEQICALLANEELRRRLGRAGRRAVEQSFCWPVA
                     WRTLEENLVQLVEDRTTVAGRTGSSW"
     gene            1897..3051
                     /locus_tag="VFA54_15225"
     CDS             1897..3051
                     /locus_tag="VFA54_15225"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZS52218.1"
                     /translation="MRFSVDAHAIGQRLTGNEVYIRTLLQGFARLDCTAKFLAYVSGH
                     DVNGWVPAHFEKRRVAANRYWRLGFDLSERLRRDRPDLIHVQYNAPLFCPVPAVVTVH
                     DISFIEHPEYFPGRRAWQLRLSTAHTLRRAAKIVTISEFSRARIASRFQIDADDIVVT
                     PLAAQEHFRPGSKEAAAFRVRERFGIGRPFVFTLGDLQPRKNQIGLIQAFRDLIFSEP
                     RIPHLLVLAGKETWFTPRIRDEIRRAGIADRVVLTGFVADADVADLYNAADLFIFPSF
                     YEGFGLPVVEAMACGRPVVCSNAGSLPEVAGRAAILVDPHSTADMASAMRTVLTDEAL
                     QARLVRQSLQRAAEFDWRETARKTLDVYYAVAEGRRRSPAPSAEPVRIAR"
     gene            3214..3957
                     /locus_tag="VFA54_15230"
     CDS             3214..3957
                     /locus_tag="VFA54_15230"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZS52219.1"
                     /translation="MNNSLVLIQVFLFELAGVWCLWAQSSPVPRGAPPAPPSTAAPFS
                     RGEVLNYSVNWPSGLSLGEAQLRVGSAEPGWDFEFTLNASLPGFEVKDHYRSEAGASF
                     CSSRLEKDFVHGSRKTKETVSYDQQKRQAERKTDAGGKSEVAISDCAKDGLTFLYYLR
                     RELASGRLPPAQVINFGAPYEISVAYGDARDVETDGVKQKADRILVSLRGPASSHSFE
                     ILFARDKARTPLEVRVPFSLGTFALELAR"
     gene            3961..6051
                     /locus_tag="VFA54_15235"
     CDS             3961..6051
                     /locus_tag="VFA54_15235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52220.1"
                     /translation="MRVAYFSPLPPRKSGIADFSAELLPHLTRHLELEVFVEDPSAVA
                     SQPVPVRRHTEYRPSDFDLALYQVGNNADHVFVYETALRHPGVVVLHEFNLHHLIAEA
                     TIRRNDWDAYLREAEYNGGEEALAYARRARALEVGPDYENLPMNRRLLEASRALIVLN
                     RFVETQARAAGYKPPLAVIPHGAAMPEGRRQAYRRRLGLDETTPLIGIFGFLKPYKRI
                     AEALRAFQRLAKVQPGVRMILVGEEHPDFSVHRLIESLGLEDRVRTLGYVPIEDFQGY
                     MSAVDICISLRYPTAGESSGPLLRALGLGRPVLVSDIGSFAELPDDVCLKVPVDGREI
                     DFIFEYLNLLVSRPELARALGERARKYVKEHCAWERVALRYAAFLQAVAEGRGWEEPS
                     GTAAEPETPQAAARSSAFAPGLIGSTPAAPAAPFPENTHPWAEYVLGYAGDSEERRNY
                     IRTHLTRLVRTLEITPRGGPQDRILEMGAYMQITPALKNLLGYGEVRGSYLGPPGRVD
                     RREARSASGECFCCLIDHFNAEKDRFPYEDNFFATVLCCELLEHLYEDPMHMMSEINR
                     ILRPGGSLVLTTPNICSLRAIEAILMAYHPGFFHQYIRPSAQGEAAPRHSREYAPRDV
                     RLLFEQAGFEVALLETGPYRSEPSAAGDWVLHLLDRYELPKDMRGEAIYSVGRKIGPV
                     RNRYPEGLYAGGAE"
     gene            6048..7277
                     /locus_tag="VFA54_15240"
     CDS             6048..7277
                     /locus_tag="VFA54_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS52221.1"
                     /translation="MIARFTGVRVDAAPAGRRIDVAAILRNESGESWKPEAGFYFSYQ
                     IFDPSSGTLLEDGRRTPLDCDVAPGGTASLRLSVPLPAQPGAYRIYVSPLQEHKAWFY
                     QSGSPFLLIDARVENGAVRLCRKKIATLSRIRWETFLRSLWRAVVYPLLTLGRHGSLI
                     RSMVRRDIAGRYRGSYAGLFWTVIHPLLMMVTYYFVFGIVLRTRFAGDDRPSSFVVYF
                     LAGMLPWLAFSEAVGRSPSVILEHRTFVKKLVFPVEILPVNLAMAGLVSEAFGAVIFA
                     AGMWYFGRPFHWTMLYLPVLIVPQLLFTLGLCWFLAALGVFFRDVAQIMGFLLTLWFF
                     VTPICYPEASLPARWMWLFAQNPLYILVRSYRMIFLEAAAPNWRALGKLAVLSLAVFF
                     AGHAWFYKLRRSFADLL"
BASE COUNT         1231 a         2236 c         2334 g         1521 t
ORIGIN      
        1 cgggaagcca tcgagcattt ccatctgtct ccggcgcgcg tggcggcggt tcctcttgcg
       61 ccgtcccggc aattccagcc gcggggcgat gaggaaacat cccgcctgct gcgccgcctg
      121 tctatcgccc ggccttattt actgtttgta ggtacgcaag agccccgtaa gaatctcggc
      181 cggctgatcg aggcctggag ggaggtccgc agggagcacc cggatttggg acttgtgctt
      241 gccggccggc ccggcgacaa tttctctttg ccggaagtgt taaatgttgc cggcgaaccc
      301 ggtctgacca tcaccggccg gctggccgac gccgatatcg ccggattgat gtcgcgcgcg
      361 gcgcttttcg tctatccttc gttgtatgaa ggctttgggc tgcctgtcct ggaggcgatg
      421 caggccggcg ccccggtggt gatttcccgg gatccggcgc tgcgcgaggt ggcgggcgac
      481 gccgcggttt ccgtcgatgc gggatccccc gtttcgttgg cttgcgcgat tgtacaattg
      541 atgaaggatc cgcgggagag aatggagttg cgggcgcgtg ggaggcgtcg tgccgcccag
      601 ttctgctggc gttacaccgc gatcagaact cacgagatct atgcagaagc tctccagcgg
      661 ttctgagtgg gcgttgctgt tggcgcccga gtcgccttat ccgatgacga gcgggggggc
      721 cgtgcggacg gccagcattt gcctcttcct ctctgctcga tttcggctgc atctggtggg
      781 gttcaaggtt ggcaacggga tggaggctgc gccggctttg ccagcgggcg cggcggaaag
      841 ggtggattgg atcagccttc ccaatcacag tagaagaatg cccgcgcggc tcgctcgaaa
      901 cgtatcccgc ctgagccgca gcgtgctgcc gctgaccgac cggttcagcg gcgcggagag
      961 catgggccgt gtggcccgaa cgcttgccgg ccggagctat cggatcgcgg tgctcgaaca
     1021 cttctggtcg gcgcattacg aggagttggt gcggcagcac gccggcctca tcgtcatgga
     1081 catgcacaat gtcgaatcgg cctttcacgc gcgctgcgct gaaacggaag catggccgga
     1141 gcgctgggcc caccgccggt tcgcggccct cgccgcacgg gaagagagcc gctggctgcc
     1201 gcgcttcgac ctggtgctgg cagcctctga ggcggacagg cagcgcattc tcggtcttgc
     1261 gcccggcgcg cgggtggccg tgtatccaaa cgccgttccc gcgcgtttcc cggaggaatg
     1321 cgcagaggaa cattgcatcg ccttttccgg aaatctcgca taccatccga ataccgccgc
     1381 tgtgcggtat ttcgcccgtg agatttggcc cgtattgcgg cggcgggacc cggccttgcg
     1441 ctggcgtctg atcggaagca acccgcaggc tgtccagcgg tggatcgccg gcaaccccag
     1501 cattgaagct actggtcccg tggaggacgc cctcgccgag ttggctcgca tccggctggc
     1561 cgttgtgccg ctgcgtgtgg gcagcggcac ccgcgtcaag attctagaag cctgggccgc
     1621 cggccgcgct gtggtttcca cacgaatcgg cgccgaaggc cttccggctg tccatggaga
     1681 gaatctgttg ctggctgatt cgccagcgga catggtcgag cagatctgcg cgttgctggc
     1741 caatgaggaa ttgcgccggc gtctgggcag agccgggcgg cgcgctgtcg agcagagctt
     1801 ttgctggcct gtagcctggc ggaccttgga agaaaacctg gtgcaactgg tcgaggatag
     1861 aaccactgtc gcgggaagaa caggaagctc ctggtgatgc gattttccgt tgatgcgcat
     1921 gcgatcggcc agcggctcac cggcaacgaa gtgtacatcc gcactctgtt gcaaggattt
     1981 gcccgcttgg actgcacggc gaagtttctg gcttatgttt ccggccatga tgtgaacggc
     2041 tgggtgccgg cgcattttga aaaacgccgc gtggccgcta atcggtactg gcgtttaggt
     2101 ttcgaccttt ccgaacgcct gcgccgcgac cggccagact tgatccatgt gcaatataat
     2161 gcgccgctgt tttgtccggt gccggccgtg gtgaccgtgc acgatatcag ttttatcgag
     2221 catcccgaat atttccccgg acggcgcgcc tggcagctcc gcctttcgac cgctcacacc
     2281 ctgcggcgcg cggccaagat cgtgaccatc agcgagttca gccgtgcgcg tatcgcgagc
     2341 cgcttccaga tcgatgccga tgacatcgtg gtgaccccgc tcgctgcgca agagcatttt
     2401 cgtcccggca gcaaagaggc ggctgctttc cgggtgcgag aacgcttcgg catcggccga
     2461 cctttcgtgt tcaccctggg cgatttgcag ccccggaaaa accaaatcgg gctgatccaa
     2521 gccttccggg acctgatttt ttccgaacct cgcatcccac atctcctcgt gctcgcaggc
     2581 aaagaaacct ggtttacgcc gcgcattcga gatgagattc gccgcgcggg catcgccgac
     2641 cgcgtcgttc tcaccggctt cgtggccgat gccgatgtcg ccgacttgta caacgccgcc
     2701 gatttgttta tttttccgtc tttttatgaa ggctttggat tgccggtggt tgaggccatg
     2761 gcgtgcggcc gtccggttgt ctgctccaac gccggatcgc tcccggaagt ggcggggcgc
     2821 gcggcgattt tagtagatcc gcattcgacc gccgatatgg ccagcgccat gcggactgtt
     2881 ctgaccgatg aagctctaca ggcaaggctg gtgagacaga gcctgcaacg cgccgccgaa
     2941 ttcgactggc gcgaaaccgc ccggaagacg ctcgatgtgt attacgccgt agcggagggc
     3001 cggcgcagaa gcccagcgcc ctctgccgag cccgtaagaa tagcgcggta gggagtcgaa
     3061 tccgtcttgg gaaacattcg gccagggcgc gggcgaacca gcttcttaga acgggtgata
     3121 gaatcgctac agcggccgcc ggagttcggt cttgtgcgcc ggtgagatgg gtgttcgttt
     3181 aaccagagga tgtgaggaag cccgcggtta cctatgaata atagcttagt cctcatccag
     3241 gtcttccttt tcgagctcgc cggggtctgg tgcttatggg cccaatcctc accggttccc
     3301 cgcggagctc cgcccgcgcc cccttcgacg gccgcgccat tttcccgggg cgaggttctg
     3361 aattattctg tgaattggcc gagcggcctc agcctgggtg aagcgcaact gagagtcggc
     3421 tcggcggagc cgggatggga tttcgaattc acactgaacg ccagcctgcc tggattcgaa
     3481 gtaaaggatc attaccggtc ggaagctggc gccagcttct gctcgagccg cctggagaag
     3541 gattttgtgc acgggagtcg caagacaaag gaaacggtct cctacgatca acaaaagcgc
     3601 caggccgagc ggaaaaccga tgccggcggg aaaagcgaag tggcgatctc ggattgtgcg
     3661 aaggatggcc tgacctttct ctactacctt cgccgcgagc tggcctccgg ccggctgccc
     3721 ccggcacagg tcattaattt cggcgcaccc tatgaaatat cggtcgcgta cggcgacgcg
     3781 cgcgacgtcg aaacggacgg cgtgaaacaa aaggccgacc ggattttggt ttctctgcga
     3841 ggaccagctt ccagccacag cttcgagatt cttttcgccc gcgataaggc ccgcactcca
     3901 cttgaggtcc gcgtcccgtt cagtttggga acctttgccc tcgagctggc gcgctgattt
     3961 atgcgggtgg cgtatttttc tcctctgccg ccgcggaaaa gtggaatcgc cgatttttcc
     4021 gccgagttgc tgccccactt gacccgtcat ctggagctgg aggtctttgt cgaggaccct
     4081 tcggcggtgg cctcgcagcc agtccccgtg cgccgccaca cagagtacag gccgtctgac
     4141 tttgatttgg ccctatacca ggtcggtaat aacgcagatc acgtctttgt ctacgaaacg
     4201 gcgctccggc atccgggagt cgtcgtgctg cacgaattca atcttcacca cctgatcgcc
     4261 gaagccacca tccggcgcaa cgattgggac gcctatttgc gcgaggcgga atacaacggc
     4321 ggggaggagg cgctggccta cgccagacgc gcgcgggcgc tcgaagtcgg ccccgattat
     4381 gagaacctcc ccatgaaccg gcggctgctc gaagcgagcc gcgctttgat cgttctcaac
     4441 cgcttcgtcg agactcaggc gcgggccgcc ggttataagc cgcccctggc cgtgattccc
     4501 cacggggcgg caatgcccga gggccggcgc caagcttatc ggcgccggct cggcttggat
     4561 gagacaacgc ccctcatcgg catcttcggc ttcctgaagc cgtacaagag aatcgcggaa
     4621 gcgctgcgcg ccttccaaag actcgcgaag gtccagcccg gagtccggat gattctggtg
     4681 ggggaggagc acccggattt ttccgtccac cgcctcatag agtcgctcgg actcgaggac
     4741 cgtgtacgga cgttgggata cgtgccgatc gaggactttc aggggtatat gagcgccgtg
     4801 gacatatgta tcagcttgcg gtatccaacc gcaggggaaa gttccggccc gctgttgcgg
     4861 gcgctcgggc tggggcggcc ggtgctggtg tcggacatag gatccttcgc cgaactgccg
     4921 gacgatgttt gtttgaaggt tcccgttgac gggcgcgaaa tcgattttat cttcgaatat
     4981 ttgaatctgt tggtcagccg ccccgagttg gcgcgggcgc tcggagagcg cgccaggaaa
     5041 tatgtcaaag agcactgcgc gtgggagcgc gtggcgctcc ggtacgcagc tttcttgcaa
     5101 gctgtggcgg aagggcgcgg ctgggaagag ccgtccggga ctgcggccga gccagaaacg
     5161 ccacaggcgg ccgcccgctc atcggccttc gcgcctggtc tgatcgggtc cacgcctgcg
     5221 gccccggcgg cgccgtttcc ggaaaatacg catccctggg ccgaatacgt cctgggatac
     5281 gccggggatt ccgaagaaag gcgcaattac attcgcacac atttgacccg cctggtccgc
     5341 acgcttgaaa tcacgccccg cggcgggccg caggaccgca ttctggagat gggggcgtac
     5401 atgcaaatta cgcccgctct caagaacctt cttggatatg gcgaagtccg cggcagctat
     5461 ctcgggcctc cagggcgcgt ggaccgccgc gaagcccggt ccgcctcggg agaatgtttt
     5521 tgctgcctta tcgaccattt caacgcggag aaagaccgct tcccgtacga ggacaatttc
     5581 ttcgccaccg tgctctgctg cgaattgctc gaacatctgt acgaagaccc catgcacatg
     5641 atgagcgaga tcaatcgcat tttgcggccg ggcggcagtc tggtattaac tacgccgaac
     5701 atttgcagcc tgcgagcgat cgaagcgatc ctgatggctt atcatcccgg ctttttccat
     5761 caatacatcc ggccctcggc ccaaggcgag gctgcgcccc gccacagccg cgagtacgcg
     5821 ccgcgtgacg tcaggctgct tttcgagcaa gccgggttcg aggtcgccct cctcgagacc
     5881 ggcccgtacc gatcggagcc gagcgcggcc ggagactggg tattgcatct cctggaccgg
     5941 tacgagctgc cgaaggatat gcgcggcgag gcgatctact ctgtgggacg caagatcggg
     6001 ccggtccgga accgctatcc cgagggcctt tacgcaggag gcgcggaatg atcgcccgct
     6061 tcaccggcgt tcgcgtggat gctgctcccg ccgggcggcg gatcgacgtc gcagcgattt
     6121 tgcgcaacga aagcggcgaa agttggaagc ccgaggccgg cttttacttc agctatcaga
     6181 ttttcgaccc ttccagcgga accctcctgg aggacggccg gcgcaccccg ctcgactgcg
     6241 acgtggcccc gggcgggacc gcatcgctgc gtctttcggt gccgctgccc gcccagccgg
     6301 gagcgtatcg gatttacgtt tccccactac aggaacacaa ggcatggttc taccagagcg
     6361 gctcgccgtt tctgctgatc gacgcccgcg tcgagaacgg cgcagtccgc ttgtgccgca
     6421 agaagatagc gacgctctcg cgcatccgct gggaaacgtt tcttcgcagc ttgtggcgcg
     6481 ctgtggtcta tccgctgctg acgctcggcc gccacggctc gctcatccgc tcgatggtgc
     6541 gccgggatat cgccgggcgc tatcgcggct cttacgccgg gctgttctgg acggtaatcc
     6601 accctctgtt gatgatggtg acttactatt ttgtattcgg catcgtgctg cgcacgcgtt
     6661 tcgccggaga cgaccggccc tccagctttg tggtgtattt tctcgccggg atgctgccgt
     6721 ggcttgcctt cagcgaggcc gtgggccgct cgccttcggt gattctggaa catcgcacat
     6781 tcgtcaagaa attggtgttt ccagtggaaa ttctgccggt caatctggcg atggccggct
     6841 tggttagcga ggcctttgga gccgtgatct tcgccgccgg catgtggtat ttcggccggc
     6901 ccttccattg gacgatgctg tatctgcccg ttctgatcgt cccgcagctc ctgtttacac
     6961 tgggactatg ctggttcctg gcggccctgg gagtgttttt ccgcgacgtc gcgcagatca
     7021 tgggcttcct gctcacactg tggttttttg tcacgccgat ttgttatccc gaggcttcat
     7081 tgcccgcgcg gtggatgtgg ttgttcgccc aaaacccctt gtacattctg gtgagaagtt
     7141 atcgcatgat ttttctggaa gccgccgccc cgaactggcg cgcgctgggc aagctggccg
     7201 tgctcagcct ggcagtattc ttcgccggcc atgcctggtt ttacaagctg cggcggtctt
     7261 ttgccgacct gctgtgagaa acgcgcgggc gaagcggccg cgcgggactc ccctcaaaag
     7321 ca
//
LOCUS       DASREK010000281         6002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1954493, whole genome shotgun sequence.
ACCESSION   DASREK010000281 DASREK010000000
VERSION     DASREK010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954493"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFA54_15245"
     CDS             complement(<1..273)
                     /locus_tag="VFA54_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HZS52222.1"
                     /translation="MAAGEEALAIQIRPLAADAELTEAVRLQKSIWGFEDVDLLPRRL
                     FVVAGKIGGQILGAFDGGRMVGFCLAIPGLKPGGSYYLHSHMLGVLP"
     gene            complement(249..1052)
                     /gene="mazG"
                     /locus_tag="VFA54_15250"
     CDS             complement(249..1052)
                     /gene="mazG"
                     /locus_tag="VFA54_15250"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZS52223.1"
                     /translation="MIIKDSLMAGEKFQKLVEIMARLRSPEGCPWDREQTFDTIKSCL
                     LEETYEVIDAIDRRDWNELAGELGDLQLQVVFFSQIAAEQGLFTIEDVLERIAAKLIR
                     RHPHVFAGESAETAGEVLKRWNELKAEERGRDGGSPALLDSVPRTLPALLEAYQLSSR
                     AAQAGFDWQRFEDLVEKLHEELAELDEARQRKDGPAPVEDELGDLLFMVVNIVRFLRF
                     DPELALRKTNRKFRERFAQVEEGLTRRGKTPAQARIEEMEELWQQAKKR"
     gene            complement(1344..1790)
                     /locus_tag="VFA54_15255"
     CDS             complement(1344..1790)
                     /locus_tag="VFA54_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS52224.1"
                     /translation="MRFLLNAAALCAAAFLLSAADTPGKEVIFIGSQQVNAGFAKGGT
                     LLNKGAYQILTSHRVQPGEVEVHNLDTDIMYVIEGSATIVTGGTVVDGKTTAPNEIRG
                     KSINGGQTHHLTKGDILVVPEGVPHWYKQIDNKLVNYYVVKVRTGK"
     gene            2115..2558
                     /locus_tag="VFA54_15260"
     CDS             2115..2558
                     /locus_tag="VFA54_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZS52225.1"
                     /translation="MFSRTAEYALRAMVWLAEKHGLPQTTQQIAAASQAPPDYLAKVL
                     QLLGRAGLVRAHRGLHGGFSLARPPERLTILQIVNAVDPIRRISECPLQLDAHAVHLC
                     PLHRRLDEAMAMIEKALASSTLAELIEAPAGSQPLCNVSAAGAEA"
     gene            2555..3904
                     /locus_tag="VFA54_15265"
     CDS             2555..3904
                     /locus_tag="VFA54_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52226.1"
                     /translation="MRKTGRGFLAAVLVALAAFSVAPIAGDSLGSFLMPFDDDVIRYR
                     SPEARDPVARLQPRIDQGEVKLSFNPRHGYLDAVLRALNVPVSSQVLVFSKTSFQAAH
                     ISPQAPRALYFNDDVYVGWVQGGDVVEVASVDPEKGGIFYTLAQQNASRPKFARRDDC
                     LQCHAAPPTLGVPGFFLRSVYPGPDGTPLLQAGSFDTTDHSPMNERWGGWYVSGVHGK
                     QLHMGNVVAKDPANPEQLDRVAGADVTDLRKLLDVTPYLTPHSDIVALMVLEHQVRLH
                     DLITRVNYETRIVESEQQVLARQYGETFGVWSESARRRVNRPVEVLLQDMLFTGEALL
                     TDPVKGTSNFQAEFEQRGPKDHLGRSLRQFDLRRRLFRYPCSFLIYSEAFDSLPKVAK
                     DRLYRRLWEVLTGRDESPAFAALSSADRQAIFEILLETKPGLPDYWKQAPAAPGSGS"
     gene            4059..5087
                     /locus_tag="VFA54_15270"
     CDS             4059..5087
                     /locus_tag="VFA54_15270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZS52227.1"
                     /translation="MGFALEQTVGNYEFLDALETSKGSRTYKVRNLVAQRIERLKVFP
                     KEMQHDRERSERFLREIKIHASLSHPNIAAFYDASYLDGQLVMTTELVEGSPLTQLLA
                     AGPIGPRDAVHYARQVLAALAYAHEHGVIHRMISPAIAIITPEKSVKLTGFELAKTLT
                     DPQLTQAGVIMGELEYMSPEQVKGVSTLDARSDIYSAGAVLYEMVAGKPPFESNSQFE
                     LMMAHVQKQPDPPSQIRPDVPPALEKIILTALAKDPAGRFQTAKEFQDALEKLEASLG
                     TAETSPHTLPPLARRQAPGAPQASDFSAALMTKDPPLFSRDLIVAGVFIFFVVAAVFF
                     AFLTATKR"
     gene            5295..>6002
                     /locus_tag="VFA54_15275"
     CDS             5295..>6002
                     /locus_tag="VFA54_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS52228.1"
                     /translation="MSDQQLKGRVAVITGASRGLGKQMAEALAAEGAAVALVARNRDL
                     LEAVKEGIVQRGGKAAAFSADVACEPEVAALPERIRAELGPPDILINNAGINVRKPIH
                     EFTLEEWRRVMQTNLDGPFLFTRALIPGMIERKFGRIINMTSIMSHVSLPGRAAYSAS
                     KFGLLGMTKALALELAPYSITVNGISPGPFATEMNTALMQDPARNAEFISKIPLGRWG
                     RVEEVGALAVFLCSEAAG"
BASE COUNT         1089 a         1926 c         1829 g         1158 t
ORIGIN      
        1 cgggagcacg cccagcatgt ggctgtgcag gtagtagctc ccgcccggct tgaggccggg
       61 gatggccaga cagaagccga ccatccggcc gccgtcgaag gctcccagga tctggccgcc
      121 gattttgccg gccaccacga aaaggcggcg cggcagcaga tccacgtcct cgaaccccca
      181 aatactcttt tgcagccgga ccgcctcggt aagctcggcg tcggcggcca gcgggcgaat
      241 ctggatggct aacgcttctt cgcctgctgc cacagctctt ccatttcctc gatgcgggct
      301 tgcgcgggag ttttgccccg ccgggtaagg ccttcttcca cttgcgcgaa gcgctcccga
      361 aatttgcggt tggtcttgcg aagcgccagc tccgggtcga accggagaaa ccggacgatg
      421 ttcaccacca tgaaaagcaa atctcccagt tcgtcttcca ccggcgccgg cccatccttg
      481 cgctgccggg cctcgtccaa ttccgccagc tcctcgtgca gcttttcaac caggtcttcg
      541 aagcgctgcc agtcgaagcc ggcctgcgcc gcgcgggagc tcagttgata ggcttccagg
      601 agcgccggca gcgtccgggg cacgctgtcc agcagcgctg gagagccgcc gtcgcggccg
      661 cgttcctcgg ccttgagctc gttccagcgc ttcagcacct cgcccgcggt ttccgcagat
      721 tcgcccgcaa acacgtgcgg atgccggcga atcagtttcg ctgcaatacg ctccaggacg
      781 tcttcgatgg tgaacagccc ttgctccgcc gctatttgcg agaaaaagac gacttggagc
      841 tggagatcac ccaactcgcc ggcaagctcg ttccaatccc ggcggtcgat ggcgtcgatg
      901 acttcgtagg tttcctccag caggcagctc ttgatcgtat cgaaggtctg ctcgcggtcc
      961 cacgggcaac cctcaggact gcgcagccgc gccatgattt cgaccagttt ctggaatttt
     1021 tccccggcca tgagcgaatc tttaattatc acacgggccg ccagcccaac gctttccctt
     1081 ggccgccact gggccgcccg agtcagtctg tagtattaat aggaacgcgg gggctgcaaa
     1141 aacgtccccg gtacaagcgc gggcagggcg agccgttttc ctttgcgccg ggccggcagg
     1201 gtggcgctcg aaagcggggc ggccgccttt ggccggttga acagaacagc ggagcgggat
     1261 cggcttgctg ccgcgcatcc gcctggcggg gcagggccgc atccggcgcg cgccgccgtg
     1321 ctttgcaacg tcagggcggc gcgctacttt cctgtgcgaa ccttcaccac gtagtaattc
     1381 accagcttgt tgtcgatctg cttataccag tgcggcacgc cttcggggac caccagaatg
     1441 tctcccttgg tcaagtgatg ggtttggccg ccgttgatgc tcttcccgcg gatttcattg
     1501 ggcgcggtcg ttttgccgtc aaccactgtg ccaccggtca ctatggtcgc cgagccctcg
     1561 atcacgtaca tgatgtcggt atccaggtta tgaacttcca cttcgccggg ttgcacgcgg
     1621 tggctggtca gaatctggta ggcgcctttg ttcaacagcg tcccgccttt ggcgaagccg
     1681 gcgttcacct gctggctccc gatgaagatg acttccttcc cgggcgtatc ggcagcgctt
     1741 aacaagaaag ccgccgcgca taaggctgct gcgttcagca aaaatcgcat cgtgtcttcc
     1801 tcctggtgct cccgtggaac ggctgggcgg aagcattcta ccagatcccc gcccgaggga
     1861 cgcggctggg ctgctggccg tggttctgct ccatcccgct tggtccttcc ccagcaaaag
     1921 ggatgaagca cgcggtgaaa agctgcgtct tgggccagtg gccggacgca agaatgcgat
     1981 gggcgccggg tcggcagggg aaattcgctc taaactgccg ggacggacgg aaaaaatcgc
     2041 gcaccggcag attgtgttga caaaagctca ccatgggagc aaactagata tgtatatcca
     2101 catatcggtt gattatgttt tcccgaacgg ccgagtacgc cctccgcgct atggtctggc
     2161 tggccgaaaa acacggcctg ccgcagacga cccagcagat tgccgccgcc agccaggcgc
     2221 cgccggacta tctcgcgaaa gtcctgcagt tgctcggcag ggccggcctg gtgcgcgccc
     2281 atcgaggtct gcatggcgga ttctctctcg cgcggccgcc ggagcgcctc acgatccttc
     2341 agattgtcaa tgctgtggat cccatccgca gaatttccga atgtcctctg caattggacg
     2401 cgcacgcggt gcatctctgc cccctgcaca ggcgtctgga cgaggcgatg gccatgatcg
     2461 aaaaggcgct tgcaagtagc accctggcgg aattgattga agctcccgcg ggatcccagc
     2521 cgctctgcaa cgtttcggcc gcaggagcgg aggcatgagg aaaacaggtc gaggtttcct
     2581 ggcggctgtg ctggtggctc tggcggcatt ctccgtcgcg cctattgcag gagacagtct
     2641 ggggtccttt ctcatgccct ttgacgatga cgtgatccgc taccggtctc ccgaggcccg
     2701 tgatccggtg gcgcgcttgc agccccggat cgatcagggc gaagtgaagc tttcgttcaa
     2761 tccccggcat ggttacctcg atgccgtcct gcgagcgctc aatgtgcctg tctcatcgca
     2821 ggttctggtg ttttccaaaa ccagttttca ggcggcccac atctcgccgc aagcgcctcg
     2881 ggcgctctat ttcaacgacg acgtctatgt gggctgggtc caaggcgggg atgtcgtcga
     2941 agtggcgtcg gtggaccccg agaagggcgg tattttctat acgctggccc agcaaaatgc
     3001 cagtcgcccg aagtttgctc ggcgcgacga ctgtctccag tgccacgccg cgccgccgac
     3061 gctgggcgtg ccaggctttt tcctgcgctc ggtatatccc ggtccggatg gaacgccttt
     3121 gctgcaagcc ggatccttcg acaccacgga tcacagccct atgaacgagc gctggggcgg
     3181 ctggtatgtg tccggcgtcc acggcaagca gcttcacatg ggaaacgtag tcgccaaaga
     3241 ccccgccaac cccgagcaac tggaccgggt ggcgggcgcc gacgtcaccg acctcagaaa
     3301 gctcctggac gtcacgcctt atctcacgcc gcacagcgat atcgtggcat tgatggtcct
     3361 ggagcaccag gtgcgcctgc acgatctgat tacgcgcgtc aattacgaaa cgcgaatcgt
     3421 cgagagcgag cagcaagtgc tggcgaggca gtacggagag acgttcggag tgtggtcgga
     3481 gtcggcccgg cggcgggtga accgcccggt ggaggtgctg ttgcaagata tgctctttac
     3541 cggagaagcg ctcctgacgg acccggtgaa agggacctcg aactttcagg ccgagtttga
     3601 gcagcgcggg ccgaaagacc atctgggccg gtccctgcgg cagtttgatt tgcggcgccg
     3661 gctttttcgc tatccatgca gtttcctgat ctactccgag gctttcgaca gcctgccgaa
     3721 ggtcgccaag gaccgcctgt accgccgcct gtgggaagtc ctcaccggcc gggacgaaag
     3781 cccggcgttc gccgcgctct cctccgccga tcgccaagcc atcttcgaaa tcctgctcga
     3841 aaccaagccg ggtctgcccg attactggaa gcaggcgccc gcggcgccag gctcgggatc
     3901 ataacgcccg cgccgttggg attctccagc cttactccgc ttctagacat acgaaacgcg
     3961 ggccgaaatc agcgataaac cttattgccc gcccgtgcac gtatggccga gactcgggga
     4021 gcaggccgcg cgcgtccgtt gactcgcggc gctcctttat gggatttgcg ctggaacaaa
     4081 ctgtcggcaa ttacgaattt ctcgatgccc tcgagacctc gaagggcagc cgcacctaca
     4141 aggtcagaaa tctggtggcg cagcgaatcg agaggctcaa agtctttccg aaggagatgc
     4201 agcacgaccg ggagcggtcc gaacggtttc tgcgggaaat caagattcac gccagccttt
     4261 cgcatcccaa tatcgccgcc ttctacgacg ccagctatct cgatggccag ttggtgatga
     4321 cgaccgagct cgttgaaggc tccccgctaa cgcagcttct ggccgccggg ccgatcggac
     4381 cgcgcgatgc ggttcattac gcacgccagg tgctggcggc gctcgcctat gcgcacgagc
     4441 acggcgtcat tcaccgaatg atttcaccgg cgatcgccat catcactccg gaaaagtccg
     4501 tcaagctgac cggcttcgag ctggccaaga cgctcacgga cccgcagctt acgcaagccg
     4561 gcgtaatcat gggcgaactg gagtatatgt ctcccgagca ggtgaagggg gtttccacgc
     4621 tcgacgcccg cagcgacatc tactcggcag gcgcggtctt gtacgaaatg gtcgccggca
     4681 agccgccctt cgagtccaac agccagtttg agctgatgat ggcgcacgtg cagaaacagc
     4741 cggatccgcc gagccaaatc cggccggacg tgccgccggc gctcgagaaa atcattttga
     4801 cagctctggc taaagatccc gctgggcgct ttcaaaccgc gaaggaattt caggatgcgc
     4861 tcgagaagct ggaagcgtcg ctcgggaccg cggagacctc cccgcacacg ctgccgccgc
     4921 tggcgcgccg ccaagcgccc ggcgctcccc aagcctccga tttctccgcc gccttaatga
     4981 ccaaggaccc gccgctgttt tcgcgggacc tgatcgtagc cggggttttc attttctttg
     5041 tcgttgcggc cgtctttttc gccttcctca ccgccaccaa gcgctaaaaa cacgagcggc
     5101 gccagcgccg ccgttcaagt gttgcgattt gaaaatctaa ccgcccgagg cgccccgggg
     5161 gccattcttt cgccgttcta ttcagcggct tcgccagggc cggccttccc cgtgccgcaa
     5221 aaaagctcct ccggctctgg cctccaagcc cggtgcggga gttgtgttaa cctcgcattt
     5281 tcaagggggc ctcattgagc gatcagcaat tgaaaggccg ggtggccgtc ataaccggcg
     5341 ccagccgcgg gctgggcaag cagatggccg aggcgctggc agcggaaggc gccgccgtgg
     5401 cgctggtggc gcgcaaccgg gacctgctgg aagccgtgaa ggaagggatc gtccagcgcg
     5461 gcggcaaagc cgccgccttt agtgcggatg tagcatgcga gccggaggtg gccgcgcttc
     5521 cggagcgtat ccgcgccgag ctcgggccgc ctgacattct gatcaacaac gccggcatca
     5581 acgttcgcaa gccgattcac gaattcaccc tagaagaatg gcgccgggtg atgcaaacca
     5641 atctcgacgg gccttttctg ttcacccgcg cgttgatccc cggcatgatc gaacgcaagt
     5701 tcggccggat catcaacatg acttcgatca tgtcccacgt ctcattgccc gggcgggcgg
     5761 cctacagcgc gagcaaattc gggctgctcg gcatgacgaa agcgctggcc ctcgagctcg
     5821 cgccctatag cattacggtc aacggcatca gcccggggcc cttcgccacc gaaatgaaca
     5881 ccgcgcttat gcaagaccct gcgcgcaacg ccgagtttat ttcgaaaatt ccgctcgggc
     5941 ggtggggccg ggtggaagag gtgggtgcgc tggccgtttt cctctgctcg gaagccgccg
     6001 gc
//
LOCUS       DASREK010000282        13466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1463616, whole genome shotgun sequence.
ACCESSION   DASREK010000282 DASREK010000000
VERSION     DASREK010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13466
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463616"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFA54_15280"
     CDS             complement(<1..679)
                     /locus_tag="VFA54_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HZS52229.1"
                     /translation="MAQDYLEIRGARVHNLKNICLKLPHNRITVITGVSGSGKSSLAF
                     DTIYAEGQRRYVESMSAYARQFLERIAKPDVEEIIGIAPPVAIRQKNTTRNPRSTVAT
                     ATEVYDFLRLLFARAGRTYCSQCGARVKKDTVDEIAGQVLQLEPGSRWYVLFPVRGTG
                     DCVSTETVKTHLFELRKKGFNRLFQQGRTFEFSTPESLLEIDFQRPLFALADRLALPQ
                     NASATPAG"
     gene            complement(824..1714)
                     /locus_tag="VFA54_15285"
     CDS             complement(824..1714)
                     /locus_tag="VFA54_15285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52230.1"
                     /translation="MQPRLSVVIASVNGLGCILECLEKLEALPERRQMEIIVLDRRTD
                     NTADVIAERFPDVVLFRGLTGKSIPELRWQGMQAAAGEIVAIIEDHCMVLPGWATEVL
                     RRVGPEYSVVGGPVENGSTERLIDWAFFLAEYGPCMPPLPEGPAEWVPGNNAAYRRRF
                     LPLQEPQWAGLWESFLQNELRKRGLRIYLSPAMLIYHKKSFRLGEMLAQRFLYSRSFA
                     AMRAKEMDSAQRRRYVLLSFALPAVLLGRIWRCVSQKRRNRREFLLGLPIIILFVISW
                     AVGEMVGYIAGPGDSLARVE"
     gene            complement(1714..2610)
                     /locus_tag="VFA54_15290"
     CDS             complement(1714..2610)
                     /locus_tag="VFA54_15290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52231.1"
                     /translation="MNRARRFSVIVASFNAPVTLERCLESLVEQVGEEGEIIVADCSD
                     EDPRRSLRRALEGVQFLRFEKRLTIPEMRREALRRAAGEILLLTEARLVPSKTWAAEL
                     LRAHQNHPQSPAIGGPIDSVPRSAFDAAVFLCEYGWHMPPAADGECTELSGANLSYKR
                     WALLECWDLVEAGAWEPFLHRRLEQKGYRLRRAGRAIVTYQNSLTVSQFLRQRFHYGR
                     WFAAARVAGTGPLRRLAYALFSPLLPLLLTCRLARVAANRGRSALLARALPWVLFSQT
                     MWSAGEFCGYLAGKGASDREVF"
     gene            complement(2607..3401)
                     /locus_tag="VFA54_15295"
     CDS             complement(2607..3401)
                     /locus_tag="VFA54_15295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS52232.1"
                     /translation="MALPEPQAPHIFSPEYYRKLDRLEQRHPWSAHMQQLALVLIGRF
                     CATPERVLDAGCGAGYFTALWREQSRPRLAVGIDASFEGLGLCRGRGLQEVAAASVQA
                     LPLCSEIFDVIYCADVLQHLSAPRAREAISELARVLRPGGIVVIRAAARRGIGSKKHR
                     DTEDYQQWEPRKLAAYLEPYGFQVEWMSLVNWLPSLAADIRGYQKRAPAGDVGLQAAA
                     GIPEGARGGVKGRLLAAYWAVERFAVLRLGARLPGGHTLLCVARKR"
     gene            complement(3409..4812)
                     /locus_tag="VFA54_15300"
     CDS             complement(3409..4812)
                     /locus_tag="VFA54_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HZS52233.1"
                     /translation="MSAARTVLQNFFNLSAGELVARLVNFAAFAHLARVLGSTDFGHV
                     GFLMTVTSYLLIPVMQGFDSVGVRDVARDRGRVRQYAGSILSIRLLSASFTWTAAAVA
                     VALLQPAPPMGLLALVFALALFPNAVSLKWVFQAVEETRPVAAASIAAQIAFAAGAFT
                     ISGPQQLVLVPVYSIIGESAGALVLIGAFARRFGPLRPIFHRPLWKELFRESAPLAAS
                     TILGTLLFNFDVLALAKFQTQAAVGLYTAIYKLVLLFATLLTLFQLSLFPLLSRAYAD
                     NRELRPVAGRVLHFVAAAFVPLAFAGTLLARPLLEFFFGPEYAAGGATLQILLWSLPV
                     MALRSVFRIILVSYNLQRLDMLAVLAGALANISLDIVLAPRLSTVGTAISTLASELTI
                     LALCYRYVWKRIEPIGIGRHCLRPAAASLVMLAAGSAMTQAPLPAQAAAAGFVYLAVL
                     LLLRGLNWKEIAALYRG"
     gene            complement(4809..6167)
                     /locus_tag="VFA54_15305"
     CDS             complement(4809..6167)
                     /locus_tag="VFA54_15305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52234.1"
                     /translation="MPAKRAPVLISVIIPVRNGERELERCLAALAQSDFADYECIVVD
                     DGSTDGSAAVAERHGCRLIRCPSRGPAAARNLGAREACGRILFFIDADVCVHPDSLSR
                     VAENFAGDRALDAVIGSYDDSPESPDFLSQYKNLMHCFVHQAGNEAASTFWSGCGAIR
                     REIFEKYSGFNEDYQRPAIEDIELGYRLTAAGRKILLDKNLRVKHLKAWSFWRLLKTD
                     IFDRGIPWTELILRDANMPNDLNVQISQRISVALVYIMLGAVGAMAVHFKGLFLVPLL
                     LLLVLLLSGYWFHEWRASGQRVRLLVPIAGLFGASIYFAWRHHMLALIPPLAVTLALI
                     YLQHRFLEGSRSARIYSFLTGICLAGTIVFILSFMPYHPMIILPFVILMLVLFINSQF
                     YIFLQGRKNFLFALTAIPFHLLYHFYNGISFCAGMLLYWTRRKPASLPAKPASRRADP
                     VK"
     gene            complement(6345..6998)
                     /locus_tag="VFA54_15310"
     CDS             complement(6345..6998)
                     /locus_tag="VFA54_15310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS52235.1"
                     /translation="MRRTIAILVLASGAWAAFGQGRGAAPSSDRYPPLAKTDSEKRIL
                     STLEAIVKAGETYANVPAADGRILRLLTEAVNAKNVIEIGTSTGISGLWFCLALQKTG
                     GRLTTFEIDPRRAAMARAHFQNAGVDAIVTLVEGDAHRNIARLRDPIDLLFIDAEKSG
                     YSDYLHKLLPLVRPGGLILAHNVDMAPQYMKEVRADPDLETVVYMQGEGLAITLKKR"
     gene            7365..8099
                     /locus_tag="VFA54_15315"
     CDS             7365..8099
                     /locus_tag="VFA54_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS52236.1"
                     /translation="MSSCVRNMPQEQVNDTVKEIRPACAGPSAIPMPPRGQKKIRIAV
                     ADDHTIFRDGVCRLLALEPDFKVVAEARNGVEALEVLQQHRPDILLLDLRMPDLDGLA
                     ILQQIQGQALKTRIIVLTASEDERDYAYAIQLGASGFVLKQNTTDVLIEAIRKVHQGE
                     FWRSEAPKAPGRQGLLQELPLTPREQEIAALVAQGLMNKEIAARLFISDQTVKNHLHN
                     IFEKLGVSDRLELALYFIHKGGKSSK"
     gene            8270..10117
                     /locus_tag="VFA54_15320"
     CDS             8270..10117
                     /locus_tag="VFA54_15320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52237.1"
                     /translation="MAFFFRRGFLVPIWALAALHAVNLYAHAPHILLSSSYMNFIAAP
                     GTNAAPQTVLLTNSTHGRMPWTAAAATVNGGNWLNLATVGGAPSSQISGELPGIAPCC
                     GWESASFTVSVVSASLPAGVYYGTITVAAPGSPPDPNLPLAPPADNTPQIIEVALTIT
                     TTGQAAPGISVLPKSLDFSGGSGTGRSYSVNVVVSNAGGGVLTWSATTDTASGGNWLS
                     AAQLSGSVLAVTASVGSLTNGIYTGRVIITADGAANSPKIVPVTFTIKDPLPPNIQLS
                     SSSLQFSMIMGAGSPQPQKLTISNAGDVSLNWQADAATFNGGSWLAVSPNSGTDSGTI
                     TVTADPASLSPGTYAARITITAPGAVNSPAQVQVTFVINPPQPVLPSNGIVNAATFLP
                     GPLAPGEIASLFGANLGPRDPVIFSLDPATNKLPTTLGGTTITFNGVKAPLFFVSANQ
                     VNFQVPFEIAFQNSAQMVVNVAGLSPAATIVPIADAALGIFTVAGTNRAAALNQDNSL
                     NSPDNPAPVGSVIQLFVTGQGMLDTKIETGSLAPASPPFPAPRLQPVGVTIGGIQANV
                     LFAGLAPGFAGLTQINAEIPRAVIPSSNVAVSVGMGFFQSPVPALIAIK"
     gene            complement(10268..>13466)
                     /locus_tag="VFA54_15325"
     CDS             complement(10268..>13466)
                     /locus_tag="VFA54_15325"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS52238.1"
                     /translation="ARAQAVANAQIHGLVTDSSGAVVPAAQVRAIQTETGQVQSTVTG
                     LDGTYVLPNLPVGPYRLEVSAAAFNNYTQSGILLQVGNNVQINVALQVGAVTQEVQVA
                     ANAAMVETQDTSISEVIDQRRIIDLPLNGRQATDLILLSGGAAMPPNSTRVITTHDYT
                     NAVGVSVAGGQINGNNYLLDGGDHNDTHSNVNMPFPFPDALQEFSVQTNGVSARYGLH
                     PGSVVNVVTKTGTNQFHGGLFEFVRNGDFNAINFFARAQDSLRRNQFGGTIGGPIKKD
                     KIFIFSGYQGTRERTAPPNSIAFVPTPAVLRGDFSVLESAPCQSNGKAKTLLDPTNGQ
                     PFPNNFISPSRFSPPSVALLKVIPVSNDPCGRLVYSIPNPNNENQYIGRADWLQSSRH
                     SVFGRYFVADYDNPPYYQGNILTTTRSGLEERAQAAVISDTYNISATLINAFHMTYSR
                     LAINRAVSPQMPSPAGVGVNMFNVYPHYTDLSVSGYFSMGGGSNAPAYFIRNQWQWAD
                     DMDWIRGRHHLSFGMEAIAIQMNEINIQSANGTFGFNGSISNDALADFLLGKPNSLTQ
                     SNTAQIGLRQKYAGLYFQDDIKVSARLNMHAGLRWEPSLPEHDVAGRGDHFSLPDYLA
                     GRKTSVYTNAPIGLLFHGDPGIPRAYANGSWLGFAPRIGVAWDPTGSGKQSLRSSYGI
                     FFDTPESYTARDWALDSPWGNAVRLTAPAGGFADPYAGFPGGNPFPTPYPPTKDAPFA
                     PQGAYILFPLNLHHPYMQQWDLSVQRQIGANWLVTAAYLGNKATHFRTSTEANPAIYG
                     PGATLANTNQRRFLSLLNPAAGAGYSTITVMDDGLNTYYNALRLSAQHRFSRSFTVLA
                     VYTYSHVIQDAEPIANRLTGNNYANPYNRNADKSPADFDLRHNFTTSFVYESPKFANQ
                     GMNWALGNWQFSCLASAHNGFPFSVTTGVDASLSGVGLDRPDQILGQAYVKNMESMQW
                     INPAAFRQNAPGTFGVVGYNALLAPGFFDADANLTRLFTIRERYRFELRFEFFNVLNH
                     PNFNAPVTRFASSSFGQIQSAGSPRILQFAAKYSF"
BASE COUNT         2819 a         4045 c         4034 g         2568 t
ORIGIN      
        1 cgccggccgg agtggcggat gcgttttgcg gaagcgcaag acggtccgct aaggcgaaca
       61 gcgggcgctg gaaatcgatc tccagcagcg actccggcgt tgaaaattca aacgtgcggc
      121 cttgctggaa gagccggttg aatcccttct tgcgcagctc gaataaatga gtcttgactg
      181 tttcagtgga aacgcaatca ccggtgccgc ggacgggaaa cagcacgtac cagcgggaac
      241 ccggctccag ttgcagcact tggccggcaa tctcgtccac tgtatctttt ttcacgcggg
      301 cgccgcactg gctgcaatag gttcttcctg cgcgtgcgaa caagaggcgc agaaagtcgt
      361 acacctccgt ggccgtcgcg accgtcgagc gggggttgcg cgtggtgttt ttttgccgga
      421 tggccacagg cggggcaatc ccgatgattt cttctacatc cggcttggcg attctttcga
      481 gaaactggcg ggcgtacgcg gacatggatt ccacataccg tctctgccct tccgcgtaga
      541 ttgtgtcgaa ggccagcgag gattttcccg agccggaaac gcccgttatc accgttattc
      601 ggttatgcgg gagcttcagg cagatgtttt tgaggttatg aacccgtgcg ccacggatct
      661 cgaggtaatc ctgagccaaa gccggcacct acagcggcaa atctgatgag agggaaacct
      721 cccattataa caagcgcctt ctcccgggca cgtttctgta aggaaacgaa ggaaacgcgc
      781 cccatattcc aggtttttgc gggccttcgc cggctgtgag cgcctattcc acccttgcca
      841 ggctgtcgcc gggcccggcg atgtagccca ccatctcccc cacggcccaa ctgattacga
      901 ataggatgat aatcggcaaa cctagtagga actcgcgcct gttgcggcgc ttttgcgaca
      961 cacagcgcca aatgcggccc agcaggaccg cgggcaaagc gaaagaaagc agcacgtagc
     1021 gccgccgctg ggcggagtcc atttccttgg ctcgcatcgc cgcgaacgat cgggagtaga
     1081 ggaaccgttg ggctagcatt tcgcccagcc gaaaggattt cttgtggtaa atgagcatcg
     1141 cagggctcag gtaaatcctt aagccacgtt ttcgtaactc attctgtaga aagctttccc
     1201 acagaccggc ccattggggt tcctgaagcg gcaagaacct gcgccgatag gctgcgttgt
     1261 tgcccggaac ccattccgcc gggccttccg gaagcggcgg catgcaagga ccgtactccg
     1321 ccagaaaaaa ggcccagtcg atcaggcgct cggtgctgcc attttcaacc ggccctccca
     1381 cgacgctgta ctccggcccc actcgccgca ggacctcggt cgcccagccc ggcagcacca
     1441 tgcagtgatc ctcgatgatg gccacaattt ccccagccgc agcctgcatt ccttgccagc
     1501 gtagttccgg gatggacttg ccggtcagcc cgcggaagag caccacatcg ggaaaacgct
     1561 cggcaattac gtcggctgtg ttatcggtcc ggcgatccaa cacaatgatt tccatctggc
     1621 gccgctcagg gagggcttct agtttttcca aacattcgag gatgcagccc aaaccgttta
     1681 ccgaggcaat cacgacggat aaccgaggct gcatcagaac acctcccggt cactggcccc
     1741 tttgcccgcc agatatccgc agaactcgcc cgccgaccac atggtttgag agaacaaaac
     1801 ccaaggcagc gccctcgcca atagagcgct gcggcctctg ttggcggcca cgcgcgccag
     1861 tcggcaagtc aagagaagcg ggagcaacgg cgaaaacagg gcatacgcca gccggcgtaa
     1921 tggcccagtt ccagctaccc gcgccgcggc aaaccagcgg ccgtaatgaa atctttggcg
     1981 caaaaattga gatacggtca gggaattctg ataagtgacg atcgcccggc ctgcccgccg
     2041 caggcgatat cctttctgct cgaggcgccg gtggaggaag ggctcccagg ccccggcttc
     2101 gacaaggtcc cagcactcca ggagcgccca gcgtttgtag ctgagattgg cccccgagag
     2161 ctcggtgcat tcaccatcgg cggcgggagg catgtgccac ccgtactcgc aaagaaacac
     2221 cgccgcgtcg aacgcggatc gcgggaccga atcaataggg ccgccgatag ccggcgactg
     2281 ggggtggttc tggtgagcgc gcagcagttc agcggcccag gtcttggacg gaacgaggcg
     2341 cgcctcggtt agcagaagaa tttcacctgc cgctcgccgc agcgcctccc gccgcatctc
     2401 cggtatcgtc aggcgttttt caaatcgcaa gaattgcacg ccttccaacg cgcggcgcaa
     2461 actccgccgg ggatcttcgt ccgagcaatc cgcgacaata atttcaccct cttcacccac
     2521 ttgctctacg agagattcaa gacaccgttc gagagtaaca ggagcattaa aggaagctac
     2581 aatcaccgag aaccgacggg cccggttcac cgtttcctcg ccacgcacag cagcgtgtgc
     2641 ccccccggca gccgcgcgcc cagccgcagt accgcgaacc gctccacggc ccaataggcg
     2701 gccagcagcc gccccttgac gcctccacga gcgccttccg gtatgccggc tgccgcttgg
     2761 aggcctacgt ctcccgccgg ggcgcgtttc tggtaacccc ggatgtcggc cgcaagcgaa
     2821 ggcagccagt ttacgagcga catccactcc acttgaaatc cgtaaggctc gagatatgcc
     2881 gccaatttcc gcggctccca ttgttggtaa tcttccgtat cgcggtgttt cttggacccg
     2941 atgccgcgcc gggcagccgc acggattaca acgatccctc ccggccgcag cacgcgggcg
     3001 agttcgctga tcgcttctct ggcgcgcggc gcgctcaggt gctgcaacac gtcagcacag
     3061 taaatcacgt cgaatatttc gctgcatagc ggcagggctt ggaccgaggc ggcagctacc
     3121 tcctgcaatc cgcggccccg gcataaaccg agtccctcaa aggacgcgtc gattccaacg
     3181 gccagccgcg gccggctttg ttcccgccac agggccgtga aataaccagc gccgcagccg
     3241 gcgtcaagca cgcgctcagg cgtcgcgcag aaacggccga tcagcacgag agcaagttgc
     3301 tgcatatgcg ccgaccaggg atgccgctgc tccaggcggt cgagcttccg gtaatattcc
     3361 ggagaaaaaa tgtgcggcgc ctggggctcg ggcagtgcca tgcgcggttc agccgcgata
     3421 aagggcagcg atctccttcc agttgaggcc tcggagcagc agcaagacag ccagatagac
     3481 aaaaccagcc gcagccgcct gggccggcag aggagcctgg gtcatcgcgc ttccggcggc
     3541 gagcataacc agcgaggccg ccgccgggcg caggcagtgc cggccgatcc cgatcggctc
     3601 aatccgtttc catacatacc gatagcacaa cgccagaatc gtcagctccg aggccagcgt
     3661 cgaaatcgcc gtacctacgg tactcagccg gggcgccagg acgatatcaa gagagatgtt
     3721 ggccagcgcg ccggccagca ccgccagcat atcgaggcgc tggagattat aggaaactag
     3781 gataatgcgg aacaccgatc gcagcgccat caccggcagc gaccaaagca ggatctgcaa
     3841 ggtcgctccg ccggcggcgt attccgggcc gaagaagaac tcgagcaggg gccgggccag
     3901 cagcgtgcct gcgaaagcca gcggtacgaa ggctgcggct acaaagtgca gtacccgccc
     3961 ggccacgggc cgcagttcgc ggttgtcggc ataagcccgc gagagcaatg ggaacaggct
     4021 gagttgaaat agggtcagca gcgtggcaaa cagcagcacg agcttgtaga tcgcggtgta
     4081 gagcccgaca gccgcttgcg tttgaaattt agccagcgcc agcacgtcga agttgaacag
     4141 cagcgtgccg agtatggttg aggccgcgag gggagcggac tcgcgaaaca gctctttcca
     4201 cagcggccgg tgaaagatgg gccgcaaagg gccgaaacgc cgcgcaaacg ctccgatcag
     4261 caccagcgct ccggccgatt cgccgattat ggaataaacg ggcacaagga caagctgctg
     4321 aggtcctgag atcgtgaagg ctccggcggc gaaagcaatt tgcgcggcga tgctcgcggc
     4381 ggcaacaggt cttgtttcct cgaccgcctg aaaaacccac ttgagagaca cggcgttggg
     4441 gaataaagcg agcgcgaaga ccagcgccag caggcccatc ggcggggccg gctggagcag
     4501 cgccactgca acggccgccg ccgtccaggt gaaagacgcc gaaagcaggc gaatcgacaa
     4561 gatgcttccg gcgtattggc gcactcggcc ccggtcccgc gccacgtccc ggactccgac
     4621 ggaatcgaaa ccctgcatta cgggaatgag cagataagaa gtcaccgtca tcaggaaacc
     4681 gacgtggccg aaatcggtgc tgcccagcac acgagccagg tgcgcaaagg ccgcgaagtt
     4741 taccagccgg gcaacgagtt ctcccgccga gaggttgaag aaattttgga ggacggtgcg
     4801 cgccgcgctc atttcaccgg gtcggcccgc cggctcgcgg gcttcgcggg aaggcttgcg
     4861 ggtttgcggc gggtccagta gagaagcatg ccggcgcaga acgagatgcc gttatagaag
     4921 tggtagagca ggtgaaaagg aatggcggtg agagcaaata ggaagttttt gcgcccctgg
     4981 aggaagatgt aaaactgcga attgataaac agcaccagca tcaggattac gaaaggcagg
     5041 atgatcatgg ggtggtaggg catgaagctg aggatgaaca caatcgtgcc tgccaggcag
     5101 atgccggtca aaaacgaata gatgcgagcg gaacgggatc cctccaaaaa tcggtgctgc
     5161 aagtaaatta gcgccagcgt cacggccaaa ggcggtatca gagccagcat gtgatgccgc
     5221 caggcgaaat agatcgacgc tccgaaaaga cccgcaatcg ggaccagcag gcgcacccgc
     5281 tggccggaag cgcgccactc gtgaaaccag taccccgaga gcagcagcac caggagcagg
     5341 agcagcggta ccaggaacag gcccttgaaa tgaaccgcca tcgcgcccac tgcgccgagc
     5401 atgatgtaga ccagggccac gctgattcgc tgcgagatct gcacgttcag gtcgttgggc
     5461 atgttcgcgt cgcgcagaat cagctcggtc cagggtatgc cgcgatcgaa aatatccgtc
     5521 ttcagcagtc tccagaagct ccaggccttg aggtgcttga cccgtagatt cttatcgagc
     5581 aggattttcc ggccggccgc ggtaagccgg tagccgagct cgatgtcctc gatggcgggg
     5641 cgctggtagt cctcattgaa gccgctgtat ttctcaaata tctctcgccg gatcgcgccg
     5701 cagccgctcc aaaacgtgga tgccgcttcg ttgccggcct ggtgcacaaa acaatgcatc
     5761 agattcttat actgggagag aaaatcaggc gactccgggc tgtcgtcata ggagccgatc
     5821 acggcatcca gggcgcgatc tcctgcaaaa ttctcggcca cacgactgag cgaatcggga
     5881 tggacgcaaa cgtcggcgtc aatgaaaaac aaaatcctgc cgcacgcctc ccgcgccccc
     5941 aggttccggg cggcggccgg cccccggctc gggcaccgga tcaggcggca gccgtgacgt
     6001 tccgccacgg ctgctgagcc gtcagtcgag ccgtcatcta ctacgatgca ctcgtaatcc
     6061 gcaaaatccg attgcgccag agccgccaga cagcgctcga gttcgcgctc gccgtttctt
     6121 acggggatga ttacggagat tagaacaggt gcacgcttcg caggcaagtt tccagtctat
     6181 gtgcggcccg ggagaattgc aagatgcctg cctcgaacgg cctgtcagga ccgacctgcc
     6241 gctggaatga gctcgcaatc cgccggcgat agaaacttgc cagcgggcgc ctacgccacg
     6301 gcatcaggcc ggcttctcgg ccaaaattcg ccgcagcccc ggtcttatcg ttttttcaaa
     6361 gtgatggcga ggccttcgcc ctgcatataa acaaccgttt caagatccgg atcggccctc
     6421 acttccttca tgtactgggg cgccatgtcc acgttgtgag ccaagatgag gccgcccgga
     6481 cgcaccagcg gcagcagttt gtggaggtag tcggaatagc cggacttctc cgcatcaatg
     6541 aacagcagat cgatcggatc ccgcaggcga gcgatgttgc gatgagcgtc gccctcaacc
     6601 aaggtgacga tcgcatcgac gccagcgttc tggaagtgag cgcgcgccat ggccgcgcgg
     6661 cgggggtcga tttcaaatgt ggtcagcctt ccgccggtct tctgaagagc aaggcagaac
     6721 caaagaccgg agatgccggt cgaagtgccg atctcaatca cgtttttggc gttgaccgcc
     6781 tcggtgagaa gccgcagaat gcggccgtcg gccgccggaa cgttcgcata tgtttccccg
     6841 gctttgacta tggcctccag cgtggagagg attcgcttct cggagtccgt cttggcaagc
     6901 ggcggatagc ggtcgctgga aggggcggcc ccgcgtccct ggccgaaagc tgcccatgcg
     6961 cctgatgcga gaacaaggat tgcgattgtc cgcctcatac agatacccct tcctctaagg
     7021 cctacatccc tggaaattat tatggctcgt tcctctgcaa aatcggcgct acaagactga
     7081 caggcttata aagacattcc gatgtttcgg tttttcacga tgcgcggctc gcttcatggg
     7141 tcctagcctt cttacgaata ctccaaagta caccggaggc agggtgtata cgggagtgca
     7201 cggcagtcta tttgttttag agcaaaaggc tttgatttca aagagagcat tcttggatcc
     7261 gccaagttgg cctgcgaatt gctgactaag gcgttcctcg gcgaaaggcc gccttgatga
     7321 agtactctgc gacgcgggca gaaggggtgc aagagaggtc gggtgtgtct tcctgcgtga
     7381 ggaatatgcc tcaggagcag gtgaatgaca ccgtaaagga gatcaggccc gcttgcgccg
     7441 gcccttccgc gatccctatg cccccacgcg gccaaaaaaa gatccgaatt gcagtagcgg
     7501 acgaccacac aatctttcgc gatggagtat gccggctcct ggcgctggag ccggacttca
     7561 aagttgtcgc cgaggctcgc aacggagtgg aagcgttaga agtactccaa cagcatcggc
     7621 ccgatattct cttattggac ttgagaatgc ccgatctcga tggcctggcc attctgcaac
     7681 agatccaggg tcaagcgctg aagacccgca ttattgtact gacggcatcg gaggacgaac
     7741 gggactacgc ttatgccatt caattgggcg cttccggttt cgtgctgaag caaaacacga
     7801 ctgacgtcct gatcgaggcc attcgtaagg ttcaccaggg tgagttttgg agatcggagg
     7861 ctcccaaggc tccgggccgt cagggcttgc ttcaggagct gccgcttacg ccccgcgagc
     7921 aggagattgc tgctctggtc gcacagggtt taatgaataa agagatcgcg gctcggcttt
     7981 ttatcagcga ccagacggta aaaaatcatc ttcacaacat ctttgaaaaa ctcggcgtgt
     8041 ccgaccggct cgagctcgcc ctctacttca ttcacaaggg cggcaagtct tcgaaataag
     8101 ccgggactat cgtccttatc tgacccggcg ggcgggcggc ttgaatccct gttggggagg
     8161 tccctccggc ggacggctct tttcacgccg gctcccccgc agaattcaca ttgtcggcgc
     8221 agacagactg ctatactatt tgctctcccg cgtcctccga aacaatctta tggcgttttt
     8281 cttccggcgt ggttttcttg tgccaatctg ggcgctcgca gcgctccatg ctgtgaatct
     8341 ctacgctcat gccccgcata tcctgctctc gagcagctac atgaacttca tcgccgcgcc
     8401 aggaacaaac gccgcgcccc aaaccgtcct tctgaccaac agcacgcatg ggcggatgcc
     8461 atggacggcg gccgcagcca cagtcaacgg cgggaactgg ctgaatttgg cgacggtcgg
     8521 cggcgccccc tcttctcaga tcagtggcga attaccggga attgcgcctt gctgcggctg
     8581 ggaaagcgcc agtttcacgg tctcggtggt atcggcttct ttgcccgcgg gcgtctatta
     8641 cggtacgatc acggtcgccg ctccgggatc gcctcccgat cctaacttgc cgctcgctcc
     8701 gcccgccgac aatacgccgc aaatcatcga ggtggcgctc actattacca cgaccggcca
     8761 ggcggcgccc ggcatatctg tattgcccaa atctttggac ttctcaggcg gttccgggac
     8821 aggccgcagc tacagcgtga acgtcgtcgt cagcaacgcc ggcggcggtg ttcttacctg
     8881 gtccgcaaca accgatactg ccagcggtgg caactggctt tccgcggcgc agcttagcgg
     8941 ctccgtgctg gcagtcacgg cgtccgtcgg cagtttgacg aacggaattt acacaggccg
     9001 cgttatcatc acggccgacg gcgctgccaa ctcgcccaaa atcgttcctg ttactttcac
     9061 aatcaaggat ccgctgcccc ctaacatcca gctcagcagc agttccctgc aattctcgat
     9121 gattatgggc gccggcagcc cgcagccgca gaagctgact atcagcaacg ccggtgacgt
     9181 ctcgctgaac tggcaggcgg acgcggcaac cttcaatgga ggctcctggc tggcggtttc
     9241 cccaaactcg gggaccgatt ccgggacaat caccgtgacc gccgacccgg cctccctcag
     9301 tccgggaacc tatgcggccc ggatcacgat caccgcgccg ggtgcggtca attctcccgc
     9361 tcaggtgcag gtaacgtttg tcatcaaccc gccgcaaccg gttcttccaa gcaacggcat
     9421 cgtaaacgcg gcgacctttc tgcccgggcc acttgcgccg ggcgaaatcg catcgttatt
     9481 cggcgccaac ctggggcctc gcgaccccgt aatcttcagc ctcgacccag cgacaaacaa
     9541 gcttcccacc acactggggg gcactacgat cacattcaac ggcgtgaagg cgcccctgtt
     9601 tttcgtcagc gccaatcagg tgaactttca ggtgcctttc gaaatcgctt tccaaaactc
     9661 cgcgcagatg gtcgtgaatg tggctggatt gagtcccgcc gcgaccatcg ttcccattgc
     9721 agacgcagcg cttggcattt ttactgtggc gggaaccaac cgggcagcag cgttgaacca
     9781 ggataattct ctgaacagtc ctgataatcc ggcgcctgtc ggcagcgtga ttcagttgtt
     9841 cgtgactggc cagggtatgc tggatactaa aatcgagact ggatccctcg ctccggcgtc
     9901 gccgccgttt cccgcgccgc gcctccagcc agtaggcgtc accataggcg gcatacaggc
     9961 gaacgtcttg tttgcaggcc tcgcgcccgg gtttgccggg ttgacccaga tcaacgcgga
    10021 aattccccgg gccgtcattc cgtccagcaa tgtggcggta tcggttggca tgggattctt
    10081 ccaatccccg gtcccggctc tcatcgcgat aaagtaaggc ggctggcccg ctcagcgatc
    10141 tgaatgtctt cagaccgatg ccagtgactg aaaccgggcc gcggccgcgc ggttgcactg
    10201 tccgcgcttc ccccgcgccg tttgacctgc cgatctcccc cactccctgc cggcggccat
    10261 ttccggctta aaaactatat ttcgctgcaa attgcaggat gcgcggcgat cctgcgctct
    10321 gaatctggcc gaacgagctg gacgcgaagc gggtcaccgg agcattaaaa ttcgggtggt
    10381 tcaggacgtt gaaaaattcg aagcgcagct cgaaccggta tctttcgcgg atcgtgaaga
    10441 gccgggttaa attcgcgtcg gcgtcaaaga agcccggcgc cagaagcgcg ttgtagccaa
    10501 caacgccgaa agtgccaggg gcattctggc ggaaggccgc gggattgatc cattgcatcg
    10561 attccatgtt cttcacgtag gcctggccga gaatttgatc cgggcggtcc agccccacgc
    10621 cggaaagcga ggcgtccacg cccgttgtca ccgagaacgg gaatccgttg tgcgccgacg
    10681 ccagacaaga aaattgccaa tttccgagcg cccagttcat gccctgattc gcgaacttcg
    10741 ggctctcata aacgaaactg gtagtgaagt tgtggcgcag atcgaaatcc gccgggcttt
    10801 tgtccgcatt gcggttgtac gggttggcat agttgttccc cgtgagccta ttggcaatgg
    10861 gctccgcgtc ctggatcacg tgcgagtacg tgtaaacggc gaggaccgta aagctacggc
    10921 tgaaccggtg ctgggccgaa agccggagcg cgttgtagta ggtgttcagg ccgtcgtcca
    10981 tgaccgtgat cgtcgagtag ccggctccgg ccgccgggtt caatagggag aggaaccggc
    11041 gctggtttgt gttggcaagg gtcgctcccg gaccgtaaat ggccgggttg gcttctgttg
    11101 aggtgcgaaa atgcgtcgcc ttgtttccta aatacgccgc cgtgaccagc caattggctc
    11161 cgatctggcg ttgcacgctg agatcccatt gctgcatgta agggtggtgc aaattcagtg
    11221 ggaacaaaat ataagcgccc tgcggcgcga aaggtgcgtc cttggttggc ggatacggag
    11281 tcgggaaggg gttgcctccc gggaagcctg catagggatc cgcaaaaccg cccgctggcg
    11341 ctgtcagcct taccgcgttg ccccagggcg agtccagggc ccagtccctc gcggtgtaag
    11401 actcgggagt gtcaaagaag atgccgtagg aagaacgaag gctctgcttt ccgcttccgg
    11461 tgggatccca ggcgactccg atgcgcggcg cgaaaccgag ccagcttcca tttgcatagg
    11521 ccctggggat tccgggatcg ccatgaaaca acaagccaat gggagcattc gtatatacgc
    11581 tggttttcct tcctgccagg taatcgggca aagaaaagtg gtctcctcgc ccggcgacgt
    11641 cgtgttccgg aagggaaggc tcccaccgca ggccagcgtg catattcagt ctggcgctca
    11701 ccttgatgtc gtcctgaaaa tatagcccgg cgtacttctg gcgaagtcca atctgcgcgg
    11761 tattcgattg agtcagcgaa ttcggcttgc ccagcaggaa gtccgcgaga gcatcattgc
    11821 tgatggatcc attaaaacca aacgtcccgt tggcggattg gatgttgatt tcgttcatct
    11881 ggatcgcgat agcctccatg ccaaaggaga gatggtggcg gcctcgaatc cagtccatgt
    11941 cgtccgccca ttgccactgg tttctgatga aataagcggg cgcgttcgac ccgccgccca
    12001 tggagaaata ccccgacacg gaaagatccg tgtagtgcgg gtagacgttg aacatattca
    12061 cgcccactcc ggccgggctc ggcatctggg gcgaaacagc gcggttgatg gccaggcgcg
    12121 aatacgtcat gtgaaaggca tttatcagcg tggcgctgat attgtatgta tcgctaataa
    12181 cggcagcctg cgcccgctcc tcgaggccgg accgggttgt cgtaagaata tttccctggt
    12241 aatagggcgg gttgtcgtag tcggctacga aataacggcc gaaaacggag tgcctggagc
    12301 tttgcagcca atccgcgcgg ccgatgtact ggttctcatt gttggggttg ggaatggaat
    12361 agaccagccg gccgcaggga tcattggaaa ccggaatcac tttgagcagc gcgaccgaag
    12421 gcgggctgaa gcggctggga ctgatgaaat tattgggaaa cggttgtccg ttcgtcggat
    12481 cgagcaaggt tttggctttg ccattagact ggcaaggcgc cgattccagc acgctgaagt
    12541 ctccgcgcag cacggccgga gtgggcacga aggcaatgga attcggaggc gcggtccgct
    12601 cgcgggtgcc ttgatagccc gagaagataa aaatcttgtc tttcttgatc gggccgccga
    12661 tcgtgcctcc gaattggttg cgccgcagcg agtcttgcgc ccgcgcgaag aagttgatgg
    12721 cgttgaaatc gccgttgcga acaaattcaa acaagccgcc gtggaactga ttcgtcccgg
    12781 ttttagtaac gacgttcacc accgagccgg ggtgcaggcc gtaccgggcg gagacgccgt
    12841 tggtctgcac gctgaattcc tgaagagcat cggggaacgg gaaaggcata ttgacgttcg
    12901 agtgggtgtc attgtgatcg cccccatcca ggaggtagtt attgccgttg atctggcctc
    12961 cggcgactga aaccccgacc gcattcgtat aatcatgagt cgtgatgacc cgcgtcgagt
    13021 tcggcggcat agccgctcct cccgataaga gaatcaggtc cgtggcttgc cggccgttca
    13081 ggggcagatc gatgatgcgc ctctggtcaa tcacctcgga aatcgacgta tcctgcgtct
    13141 ccaccatggc cgcgttcgcg gcgacttgta cttcctgggt gacggctccg acctgaagcg
    13201 ccacgttgat ctggacgttg ttgcccacct gtaggagaat gccggactgc gtatagttgt
    13261 tgaaagcagc cgcgctaacc tccagacgat acggcccaac gggcaggttc ggaagaacat
    13321 aggttccgtc gaggccggtc acggtacttt gaacctggcc ggtttcggtc tggatcgccc
    13381 tcacttgggc agccgggacc acggcgccgc tcgaatcggt caccaaaccg tgaatttgag
    13441 cgtttgcaac cgcctgggcg cgcgcc
//
LOCUS       DASREK010000283         7423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1464101, whole genome shotgun sequence.
ACCESSION   DASREK010000283 DASREK010000000
VERSION     DASREK010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464101"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VFA54_15330"
     CDS             complement(<1..596)
                     /locus_tag="VFA54_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS52239.1"
                     /translation="MHPAIEVENLVKTFGRFVAVDHVSLSVERGEVFGFLGPNGAGKS
                     TTIRILCGLLSPTAGRALVNGLDVAKQPEEVRRNIGYMSQKFSLYDDLTIEENIDFFS
                     GVYGVPRPKRAARKDYVLQMAGLSEQRRRMTGLLSGGWKQRLALGCAILHEPPVLFLD
                     EPTSGVDPIARRAFWDLIYELSAAGHTVFVTTHYMDEA"
     gene            complement(661..1590)
                     /locus_tag="VFA54_15335"
     CDS             complement(661..1590)
                     /locus_tag="VFA54_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS52240.1"
                     /translation="MERAIIQAHGLTRRFGALTAVNHLDLEVERGEIFGLVGPDGAGK
                     TTTLRLLCGLMDPSEGEAWVAGSDTAREPEAVKDQIGYMAQRFGLYADLTVEENMDFY
                     ADLFGITGLGRDQLKRRLLAMTRMEPFRQRQAARLSGGMKQKLALMCTLLHRPQILFL
                     DEPTNGVDPLSRRDFWAILYQLVKDGITIFVTTAYLDEAERCNRIGMMDRGRLIHCDT
                     PEALKGQLEEICYEVEAADRDAARRILRRQPGVVSVEMSGAALHVLVSPSAAPAESLQ
                     QALPQARIRRVEPSLEDVFLALVRRARAAEGAV"
     gene            complement(1599..2780)
                     /locus_tag="VFA54_15340"
     CDS             complement(1599..2780)
                     /locus_tag="VFA54_15340"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS52241.1"
                     /translation="MKRKLILPIILVGAGIAGLVAFRGFQRQPEDKIVLSGNIELTEV
                     NIAFKTAGKLIERMVDEGDPVTKGMVIARLDRDQLLRQQERERAALAAAQMQLAQAQT
                     AVNWQQQTLHADLEQRRADLRAYEAQYEQLKNGSRPQEIQDAKAAVEAAQAVYDRARN
                     DWERAQALFKNDDISRAQYDQYRTQFDSAQAALKQAQQRADLVLIGPRQEQIEAARAQ
                     MERARAALKMAEANELELKRRNQEVSARRAEIERERAQIALIDSQLEDTVAVSPIEGV
                     VLVKAADVGEVLAPGATVVTIGDMDHPWLRGYIGERDLGRVKLGTKAKITSDSYPGKA
                     YWGRVSFISSEAEFTPKQIQTQEERVKLVYRIKIDVDNPRRELKLNMPVDAEILVGQQ
                     Y"
     gene            complement(2975..4330)
                     /locus_tag="VFA54_15345"
     CDS             complement(2975..4330)
                     /locus_tag="VFA54_15345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS52242.1"
                     /translation="MSRPAIPIQLLLLGIAPLACLAQAQPVGDGGPVRLSLKRAVEIA
                     LSPEGSARIQLSEEALRQARLRSAEARAALLPDIEGSVTEENLNRNLAAFGLQLSAPL
                     PGFQIPAVVGPFNVFDARATASQSLFDFSSIRRFQASKVAVNAAKADRQNADNEVSAQ
                     VAKAYYAALRSEAALEAANADVALAEALVKQAQDQKTAGTGTGIEITRARVQLANARQ
                     RQLAAENDRRRAHLQLLRAMNVRLDIAIELTDKLAYLPADPIAVQEATQQALNMRADW
                     KAQQQREENARLASSAVKMERLPSLTAFADYGSIGTGIDNAFPTRTYGVSLRVPVFDG
                     GRRDARRAESQSQWRQERVRTSDLREQIALDVRLALDSLRSAEDQVKVAEEGLALAEN
                     ELEQARRRYAAGVANGIEVTDAQTRLERARENRIAALYSYNLARIDLAQATGTIRSLV
                     Q"
     gene            complement(4327..4980)
                     /locus_tag="VFA54_15350"
     CDS             complement(4327..4980)
                     /locus_tag="VFA54_15350"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZS52243.1"
                     /translation="MRAAADTKTRILDTAERLFAEHGYAPTSLRKVIAEAGVNLAAVH
                     YHFHSKQDLLEAVVVRRAAPVNRERLEMLARCEREAGGKPPPVEKILEAFLAPAFRLA
                     RECTEGGAAFARLMGRLHAENDALPRILKNNFGPVLTAFHSALHRALPEVPKEELFWR
                     LYFAVGAMAHTLRGDQGLGFISKGACPTPDWQTTLRRLIDFLSAGFCAPVKVRRKRK"
     gene            5236..6129
                     /locus_tag="VFA54_15355"
     CDS             5236..6129
                     /locus_tag="VFA54_15355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZS52244.1"
                     /translation="MERKKATDFPQELLNLFDRYIHGDIGRREFLEGAKQFAVGGLTA
                     TAIWESLRPNYAWAEQVAKDDSRIKTEYATVSSPQGNGSIRGYWVRPSNAGGKAPGVL
                     VVHENRGLNPYIEDVARRLGTANFLAFAPDGLTSVGGYPGDEEKGVAAFRTVDGKKMF
                     EDFVASAYWLKSRPECTGKIGVVGFCFGGGVANNLAVRMGSDLAAAVPFYGAQPSAAD
                     AAKIKAPLLLHYASLDTRITGGWPAYEQALKANRVTYTAYVYEGANHGFHNDTTPRYD
                     EAAAKLAWQRTIDFFNKYLRS"
     gene            6300..>7423
                     /locus_tag="VFA54_15360"
     CDS             6300..>7423
                     /locus_tag="VFA54_15360"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HZS52245.1"
                     /translation="MSERSGKSKAEERRHSMETGRRRWLQLSLAAGAASALPPAKAAA
                     QAPRSAAPAARRPIDEYDPSNIKLAHRVPANIAEDDLLFLKQIGLRWARVEFGAGKVS
                     FEQIRATQEKYARFGIRIFSAVQESYRSLKIQLGQPGRDEDIETFRTFLRDIGRLGIG
                     VANIDFHPGNTYTTAIVESPRGYKTRQFRLADFRSKVEKQRFEREYPAEEIWANYTYF
                     IKAVLPAAEESNVKLALHPDDPPLEKMNGVAKIFTNYEGYHRAEQIAGGSRSWGLTFC
                     IGTWSEGGGKMGKNVFEMIRDFGGRGKIVDVHFRNVSSPLPDFVETFQDEGYLDMYQV
                     MRALREVRFSGSAVPDHVPALAGDQTIHRAGTAYCIAYMRA"
BASE COUNT         1315 a         2415 c         2286 g         1407 t
ORIGIN      
        1 tccgcttcgt ccatgtagtg cgtggttaca aacaccgtgt ggccggcggc ggagagctcg
       61 taaatcaggt cccagaaagc ccggcgggcg atgggatcca cgcccgaggt tggctcgtcc
      121 aggaacagca ccggcggctc atgcaggatc gcgcagccca gcgccaggcg ctgtttccag
      181 ccgcccgaaa gcaggccggt cattctgcgg cgctgctcgc tcaggccggc catttgcagc
      241 acgtaatcct tgcgggccgc ccgcttcggg cgaggcacgc cgtaaacgcc gctaaaaaaa
      301 tcgatgtttt cttctatcgt caggtcgtcg tagagagaaa atttctgcga catgtatccg
      361 atattccggc gcacctcctc gggctgcttg gccacatcca gcccgttcac gagcgcccgg
      421 cccgcagtgg gcgagagcag accgcagaga atgcgaatcg tcgtggattt gcccgcgccg
      481 ttcggaccca aaaagccgaa aacctcgccc cgttccacgg aaagagaaac gtgatcgacg
      541 gccacgaagc ggccgaacgt cttcaccagg ttctcgacct ctatggccgg gtgcattgtt
      601 cctcacttcg acggggctgt ggatccgctt tgagcgacac gaccccggcg ccgggccctg
      661 ctagaccgcg ccttccgcgg ctcttgcccg ccgcaccagc gctaaaaaca cgtcctccag
      721 cgacggctcg acccgccgga tcctcgcttg cggcagcgcc tgttgcagcg attcggctgg
      781 cgcggcggag ggagaaacca gcacgtgcag cgcggcgccc gacatctcga cgctcaccac
      841 cccgggctgc cgcctaagaa tcctgcgcgc cgcatcccga tcggcggcct cgacctcgta
      901 acagatctct tccaactgcc ccttcaaggc ttcgggggtg tcgcaatgga tgaggcggcc
      961 ccgatccatc atcccgatgc ggttacagcg ttcggcttcg tccaggtacg cggtagtgac
     1021 gaagatggtg atgccatctt tgacgagctg atagagaatg gcccagaaat cgcggcgcga
     1081 aagcggatcg acgccgttgg tcggctcgtc taagaacaag atctgcgggc gatgcagcag
     1141 cgtgcacatt aaagcgagct tctgcttcat gccgccggaa agccgggcgg cctgccgctg
     1201 ccggaacggc tccatacgcg tcatagcaag caggcggcgc ttcaattgat ctcgccccaa
     1261 gcctgtgatt ccgaacaaat cggcgtagaa atccatgttt tcttccaccg tcaggtcggc
     1321 ataaaggccg aagcgctgcg ccatatagcc gatctgatcc ttcaccgctt ccggttcccg
     1381 ggctgtatcg ctcccggcca cccaagcctc cccttcgctc gggtccatca ggccgcaaag
     1441 caatcgcagc gtcgtggtct tgccggcccc gtccgggccg actagaccga agatctcgcc
     1501 gcgctcgacc tccaggtcga gatggttcac cgcggtgagc gcgccgaaac ggcgcgtcag
     1561 gccatgcgct tgtatgatcg cccgctccat gctttgcgtt aatactgctg gccgaccagg
     1621 atctcggcgt ccaccggcat gttcagcttg agctcgcgcc gcggattatc gacatcgatc
     1681 ttgatccggt acaccagctt gacccgctct tcctgggttt gaatctgttt gggcgtgaat
     1741 tcggcctctg aggagatgaa gctcaccctc ccccaatacg ctttgccggg atacgaatcg
     1801 cttgtaattt tcgccttggt tcccagcttg acgcgcccca aatcccgctc cccgatgtat
     1861 ccccgcagcc agggatgatc catatcgccg atggtgacga ccgtggcgcc gggcgcaaga
     1921 acctctccga catcggccgc tttgacgagc accacgccct cgatcgggga aacagcaacc
     1981 gtatcttcca gttgggaatc gattagggcg atctgcgccc gctcgcgctc gatctccgcg
     2041 cgccgcgcgc ttacctcctg gttgcgccgc ttcagctcga gctcgttggc ctcggccatt
     2101 ttcagcgccg cgcgagcccg ctccatctgc gcgcgtgcgg cctcaatctg ctcctgccgc
     2161 gggccgatca gaaccaggtc ggcgcgttgt tgggcttgtt tcaaagccgc ctgggcgctg
     2221 tcgaactgcg tccggtattg atcgtattgc gcccgtgaga tgtcgtcgtt cttgaaaagc
     2281 gcctgggcgc gctcccaatc gttcctggcg cggtcataaa cggcttgggc cgcctcgacc
     2341 gcggctttgg cgtcctggat ttcctgcggc cgcgagccgt ttttcaattg ctcgtattgg
     2401 gcctcatagg ctcgcaggtc ggcgcggcgc tgctccaggt cggcgtgaag cgtctgctgc
     2461 tgccagttca ccgcggtttg cgcctgtgcc agttgcattt gggcagccgc cagagctgct
     2521 cgctcgcgct cctgctggcg cagcaactgg tcgcggtcga gcctggcgat caccattccc
     2581 ttggtcactg gatcgccttc atcgaccatt cgctcgatca acttgccggc cgtcttgaag
     2641 gcgatgttca cctccgtcag ctcaatattt ccggagagca cgatcttgtc ttccggctgg
     2701 cgctgaaagc ctcgaaaggc gaccagcccg gcaatgccgg ctccgaccag aataatgggc
     2761 agaatcaatt tcctcttcac tggaaatact ccctcgccgg gaacgatggc gcccgttcgt
     2821 accctctact tccgccaaac ggcatctcct gcctcgtaga agcgccccaa agacttcttc
     2881 caggacgcag tgcgaaccaa gggtttcgcc gccccgcgcc agaaccttgc gctttgcggc
     2941 tcgtactccg gcggccagcg tcacgccgct ggggctactg cactaggctg cgtatcgtac
     3001 cggtggcctg cgccaggtca atccgagcca ggttgtagct gtaaagcgcg gctatgcggt
     3061 tctcccgcgc gcgctccaac ctggtttggg cgtcggtaac ttcaattccg ttggctacgc
     3121 cggcggcata acgccgccgg gcctgctcca actcgttttc cgccaacgcg aggccttcct
     3181 ctgcgacctt gacctgatcc tccgcggaac gcaagctatc gagcgccagc cgcacgtcca
     3241 gcgcgatctg ctcccgcagg tcgctggtgc gaacacgttc ctgccgccac tgcgactggc
     3301 tctccgcccg gcgagcgtcg cgccggccgc cgtcgaacac cggcacccgg agcgacacgc
     3361 cataagttcg cgtcggaaaa gcattgtcga ttcctgtccc gatcgagccg tagtcggcga
     3421 aggcagtcag cgacggcagg cgttccatct tcaccgcgct gctcgccagc cgcgcgttct
     3481 cctcccgctg ctgctgcgcc ttccagtcgg cccgcatgtt caaggcctgt tgcgtcgctt
     3541 cttgcaccgc gatcggatcg gcgggcagat aggcgagctt atcggtaagc tcgatggcaa
     3601 tatccaggcg aacgttcatg gcgcgcagca attgcaggtg ggcgcggcgg cggtcgtttt
     3661 cggccgccaa ctgccgctgg cgcgcgttgg caagctgaac ccgcgcgcgg gtaatttcga
     3721 ttcccgtgcc agttcccgcc gttttttggt cctgcgcctg cttcaccagc gcttcggcga
     3781 gcgcgacgtc ggcattggcg gcttcgagcg cagcctcgga gcgcagcgcc gcgtagtagg
     3841 ctttggcgac ctgcgccgat acctcgttgt ctgcgttttg gcggtctgct ttggcggcgt
     3901 tgaccgccac cttcgacgcc tgaaaccggc ggatgctgct gaaatcgaac aggctctggc
     3961 tggccgtggc ccgggcatcg aacacattga agggtcccac cacggccgga atctgaaacc
     4021 ctggcaacgg ggcgctcaat tggaggccga aagccgccag gttgcggttc aaattttctt
     4081 cagtgacgga tccttctatg tcgggcagca gagcggcccg ggcctccgcc gatcgcaacc
     4141 gtgcctgccg cagggcctcc tccgaaagct ggatccgcgc gcttccttcg ggagagagcg
     4201 cgatctcgac ggcgcgcttg aggctgagcc gcaccgggcc gccatccccg accggctgtg
     4261 cctgcgccag gcaggcgagc ggcgctattc ccagcagcag cagctggatc ggaatcgccg
     4321 gccggctcat ttgcgttttc tccttacttt taccggagcg caaaatccgg cgctcagaaa
     4381 atcgatgagc cgccggagcg tggtttgcca gtcgggtgtt gggcaggcgc ccttggagat
     4441 aaaccccagc ccctggtctc cgcgcagggt gtgcgccata gctcctacag cgaaatacag
     4501 gcgccagaac agctcctcct tgggaacttc gggtaaagcg cggtgcaaag cggaatgaaa
     4561 agcggtcagg acggggccga agttgttttt caggattctc gggagcgcgt cgttttccgc
     4621 gtgaaggcgg cccatcagcc gggcaaaagc cgcgccgccc tctgtgcatt cccgcgccag
     4681 ccggaaagcc ggggccagga aggcctccag aattttctct accgggggcg gtttgccgcc
     4741 ggcctcccgc tcacaacggg cgagcatctc cagccgctcg cgatttaccg gcgcggcccg
     4801 tcgcacgacc accgcctcca gaagatcctg cttggaatga aagtgatagt gaaccgccgc
     4861 caggttcacg cctgcctcgg cgatcacttt gcgcagcgag gtgggggcgt agccgtgctc
     4921 ggcgaagagg cgttcggctg tgtcgagaat gcgcgttttc gtgtcggccg cggctctcat
     4981 tcagtcgtac gtttgaattg tacgactgaa tgatatactt ggcaagccca atgcgcaaaa
     5041 atttttgaaa cgcccttccc gcgaagggac ccgcttcggg accagggggc cccacccgca
     5101 aaccttcacc gcgagccgcg gtctatgttc gcaggcgaaa gcggactgta gggttacatt
     5161 cggattttgc ccgtcccttg acgaaccggg accgccgcta tggtatgctt tgccccggaa
     5221 aggagcccac ttcctatgga acgaaagaaa gcgaccgatt ttccacaaga gttgctgaac
     5281 ctgtttgacc gctacatcca cggcgacatc ggccgccgcg aatttctgga gggcgcaaaa
     5341 cagtttgctg tgggcggttt gacggcgacg gcgatctggg agagcctgcg cccgaactat
     5401 gcctgggccg agcaagtcgc caaagacgac agccgcatca agaccgagta cgccacggtg
     5461 agctcgccgc agggcaacgg cagcatccgc gggtattggg ttcgcccgtc caatgccggg
     5521 ggcaaagcgc ccggggtgct ggtcgttcac gagaacaggg gcctgaaccc ttacattgaa
     5581 gacgtggcgc ggcgcctggg tactgcgaat ttcctcgcct ttgcgcccga tggtctcacc
     5641 agtgtaggcg gctatccggg ggacgaagaa aagggtgtgg cggcgtttcg aaccgtggat
     5701 gggaaaaaga tgtttgagga tttcgtagct tccgcgtatt ggctgaaatc ccgccccgag
     5761 tgcaccggaa aaatcggtgt ggttggcttc tgcttcggcg gcggcgtcgc caataacctg
     5821 gcggtgcgca tggggtcgga cctcgcggca gcggttcctt tctacggggc gcagccctca
     5881 gcggcggatg cggccaagat caaggcgccg cttctgctgc actatgcgtc gctcgatacc
     5941 cgcatcaccg gcggctggcc cgcttacgag caagcgctaa aagcaaaccg cgtgacctac
     6001 acggcgtatg tttatgaggg cgccaatcac ggctttcata acgatacgac ccctcgctac
     6061 gacgaggccg cggccaagct ggcctggcag cgcacgattg acttcttcaa taagtacttg
     6121 aggagctgag ccagggtccg cgcgctgcgc cgccgcttgt gggagcacgc aggccgcggc
     6181 ggcttccggc ccttcggtga gaaccagaac ctctgcaacg cggcgtcgcg gctggcttca
     6241 gccgggcgtg catccgcacc gctgccgccc gggcgaggaa tcgggaggag ggcagaccga
     6301 tgagtgaaag atctggcaag agcaaggcgg aagagcgccg gcacagcatg gagacgggac
     6361 gccggcgctg gctgcaactg tcgctggctg ctggagcggc cagcgcgctg ccgcccgcca
     6421 aggcggctgc gcaagctccc cgctccgccg cgccggccgc ccggcgcccc atcgacgagt
     6481 acgacccgtc gaacatcaaa ctggcccacc gcgtgccggc gaacatcgcc gaagacgact
     6541 tgttattcct caaacaaatt gggctgcgct gggcccgggt ggagtttgga gcagggaagg
     6601 tttccttcga gcagattcgc gccacgcagg agaagtacgc ccgcttcggg attcgaatct
     6661 tctcagctgt tcaggagtcg taccggtcct tgaagatcca gctcggccag cccgggcgcg
     6721 acgaggatat cgaaaccttc cgcacgttcc tacgcgatat cgggcggctg gggatcggag
     6781 tggcgaacat cgatttccat cctggcaata cctatacgac agccatagtg gaaagtccgc
     6841 gcgggtacaa aacgcggcaa ttccggctgg ccgattttcg gtcaaaggtg gaaaagcagc
     6901 gcttcgagcg ggaatacccg gcggaggaga tctgggccaa ttacacttat ttcatcaagg
     6961 ctgtgctgcc ggcggcggaa gagtcgaatg tgaaactcgc cctgcacccg gatgatccgc
     7021 cgctcgagaa gatgaacggc gtcgccaaaa ttttcaccaa ctatgagggc taccaccggg
     7081 ccgagcagat cgccggcggc agccgctcct ggggcctcac cttttgcatc gggacctggt
     7141 cggagggcgg cggcaagatg ggcaagaacg tcttcgagat gatccgcgac ttcggcggac
     7201 gcggcaagat cgtcgatgtg cacttccgca atgtcagctc gccgctgccc gattttgtcg
     7261 agaccttcca ggacgagggc tatctcgaca tgtaccaggt gatgcgggcg ctgcgcgagg
     7321 tccgcttcag cggctcggcc gtgcccgacc acgtgcccgc gctggccgga gaccagacca
     7381 tccaccgcgc cggcacggct tattgcatcg cctacatgcg cgc
//
LOCUS       DASREK010000284         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_139505, whole genome shotgun sequence.
ACCESSION   DASREK010000284 DASREK010000000
VERSION     DASREK010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139505"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFA54_15365"
     CDS             complement(<1..235)
                     /locus_tag="VFA54_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZS52246.1"
                     /translation="MRVVIIGGGLAGLAAAAALGSIRGPDALEIDLYEARPFLGGRAT
                     SFPVSPSDPDSERIDNCQHVLLRCCTNLLDFYQR"
     gene            complement(400..1290)
                     /locus_tag="VFA54_15370"
     CDS             complement(400..1290)
                     /locus_tag="VFA54_15370"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HZS52247.1"
                     /translation="MLTAEASLEASQAHCRRVARRAARNFYYAFLPLSREQHNAICAV
                     YAFARRSDDFADDETPCTIEDRRSSLNRWRAALEAALAGDCGENLELPAVCAAIERFA
                     IPPLYFFELIAGVSTDLDPPRYDTFDELYRYCYSVASTIGLICLHIFGFQSQKAPSCA
                     EKLGVAFQLTNILRDLREDTKRGRLYLPQEDLQRFGIARGDIESGRIERVRELLRFEA
                     DRAETYYREGAPLLGLVDPRSRACLWVMTAIYHGILERMRAADYDVFSRRAGLSHAEK
                     TGIMIRGLKLHLLGRDKPFP"
     gene            complement(1277..2236)
                     /gene="hpnC"
                     /locus_tag="VFA54_15375"
     CDS             complement(1277..2236)
                     /gene="hpnC"
                     /locus_tag="VFA54_15375"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HZS52248.1"
                     /translation="MAFLLPVQFVSTPGATEETWSLQDSLRYTRWLARHHYENFHVVS
                     FLLPRRLHQDFFNIYAFCRWADDLGDEIGDSALSLQLLGWWRENLHKMYRGEARHPVY
                     VALRGTVERHAIPEQPFCDLIRAFVQDQTVTRYQTYAQLLDYCVYSANPVGRLVLHVC
                     GYRDAERCGLSDATCTALQLANFWQDVVRDLERGRTYIPIEVMQAHGYRPEDLEQRRF
                     NKNFRDTMRDLVDRAHMLFLEGLPLVERVDRRLAVDLELFSRGGMAILEKIRAQDYNV
                     LAQRPALSARDRLALLARAAWRAGFGRAAAGPSRSPEATDAYR"
     gene            complement(2298..3353)
                     /locus_tag="VFA54_15380"
     CDS             complement(2298..3353)
                     /locus_tag="VFA54_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZS52249.1"
                     /translation="MNVKTERTMMAAVLYGKEELRVEPVEIPTLGASDVLVKVNVALT
                     CGTDLKVFRRGYHARMIVPPAVLGHELAGDIVEVGSQVKNFQVGQRVVAANSAPCENC
                     YFCARRQENLCEDLLFNNGAYAEYIRIPGPIVRRNMYEIPPHVGYQDAALIEPLACVM
                     RGLDETHLRPGDTVVVIGLGPIGLMFVRLAKTLDARVIAIGRRKTQLDRAASMGADEL
                     ISTESVANVVEAVTRLTYRGRGADAAIEAVGQPETWQWAIRMVRSGGTVNLFGGCPTG
                     SEVSLDTALLHYSEITLKSSFHHTPSHIRRALDVISQGQIRAADFVTGEAPLSDILGV
                     LRHMMNRNGHLKTAIVP"
     gene            complement(3354..>4146)
                     /locus_tag="VFA54_15385"
     CDS             complement(3354..>4146)
                     /locus_tag="VFA54_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZS52250.1"
                     /translation="CGNCFYCRRRLYAQCSTYKRVGITAGFEPAGGGFAQYVRVMDWI
                     VRRGVVRVPDGVSMDRACFVEPVNTCYKAVVKCDVQAGDIAAVLGQGPIGLLFTMLLV
                     RRGAIVHAIDPLEGRRELARKLGAVRVFETSGEEAADAVRSASSGRGADVVLVAAAAP
                     GLAEQAVRLSRPGARILLFAQTSAMEKIEIDGASICAQERTLLGCYSADVDLQDESSR
                     LVLDGHLPVDCLVSHRLRLGQIHSGIEMATHPDSHTLKIIVHPQR"
BASE COUNT          823 a         1330 c         1282 g          711 t
ORIGIN      
        1 agcgctggta gaagtcgagc aggttggtgc agcagcgcag gagaacgtgc tggcaattat
       61 cgatgcgttc cgagtcggga tcggaagggg aaacggggaa ggatgtggcg cggccgccga
      121 gaaaagggcg agcctcgtag aggtcaattt ccaaggcatc gggacctcgg atagagccaa
      181 gagccgccgc cgcagccagc ccggcgagac cgccgccgat aatcaccaca cgcataagaa
      241 gtttcagggc cgccttcgag ccacaggtat caggcatgcg ctcgccgcgg ctcgcgcccg
      301 cgatcgatcc tcatacggag acagcaggga atttttccgg gagggacgca ggttccggcg
      361 gccgcagtcc ggccgtgcgc ccgcggcgca actctgtctt cacggaaacg gcttatcgcg
      421 tcccagcagg tgcagcttca ggccgcgtat catgatgccc gtcttctccg catgactcag
      481 ccccgcccgt ctagaaaaca catcataatc ggccgcccgc atccgttcca gaatgccgtg
      541 atatatggcc gtcataaccc aaagacaagc ccggctgcgc gggtcaacaa ggcccagcag
      601 cggcgccccc tcgcggtaat acgtttcggc cctgtccgcc tcaaagcgca gcagttcgcg
      661 gacgcgctca atgcggccgc tttcgatatc ccctcgggca atgccgaagc gctggaggtc
      721 ctcctggggc aggtatagcc gcccgcgctt cgtatcttcg cgcaaatcgc gaaggatgtt
      781 tgtcaactga aaggccacgc cgagcttttc cgcgcaggac ggggccttct gtgattgaaa
      841 gccgaaaatg tgcaaacaga tcaggccgat ggtcgaggcg acggaatagc aatagcgata
      901 gagctcatcg aaggtgtcgt aacgcggagg atccagatcg gtcgagacgc ctgcgatcaa
      961 ctcgaagaaa taaagcggcg ggatggcgaa gcgctcgatt gccgcgcaaa ccgccggcag
     1021 ctcgaggttt tcgccgcaat cgcccgcgag cgccgcttcc agcgcggcgc gccagcggtt
     1081 caggctgctc cggcggtctt cgatcgtgca aggcgtttcg tcgtcggcga aatcatccga
     1141 gcgccgcgca aaggcataga cggcgcaaat cgcgttgtgc tgctcgcggg agagggggag
     1201 aaacgcgtaa tagaaattgc gcgcagcgcg gcgcgccacg cgccggcaat gcgcctggga
     1261 agcttccaga gacgcctcag cggtaagcat cggtcgcctc cggagagcgg ctggggcccg
     1321 cggccgccct tccgaagccc gcgcgccagg ctgcccgggc gagcagcgcg aggcggtcgc
     1381 gcgcgctcaa cgccggacgc tgcgcgagca cgttgtagtc ctgcgcgcga atcttctcca
     1441 ggatcgccat gccgccgcgg ctgaacagct ccaggtctac ggccaggcgg cggtccactc
     1501 tttcgaccag cggcaagcct tcgagaaaca gcatgtgggc ccggtccacc aggtcgcgca
     1561 tggtgtcgcg aaaattttta ttgaaccggc gctgctcaag atcctcgggc ctgtagccgt
     1621 gagcctgcat cacctcgatc gggatgtagg tgcgtcccct ctcaaggtcc cggaccacgt
     1681 cctgccagaa attggcaagc tggagcgcgg tgcaggtggc gtcagagaga ccgcatcgct
     1741 cagcgtcgcg gtagccgcag acatgaagga ccaggcgacc gacggggttg gcggaataga
     1801 cgcagtaatc gagcaattgg gcgtaggtct ggtagcgggt cacggtttgg tcctgcacaa
     1861 aggcgcgaat caggtcgcag aacggctgct cgggaatcgc atgccgctcg acagtcccgc
     1921 ggagcgcaac gtacaccggg tggcgcgctt caccgcggta catcttgtgc aggttctccc
     1981 gccaccagcc aagcagttgc aggctcagcg cggagtctcc gatctcgtcg cccaaatcgt
     2041 ccgcccagcg gcagaaggcg taaatgttga aaaaatcctg gtgcagccgg cgcggcagca
     2101 aaaaactgac tacatgaaag ttttcgtagt gatggcgcgc cagccagcgc gtgtaccgga
     2161 gcgagtcttg caggctccag gtttcctcgg tcgcgcccgg cgtggaaacg aactgaactg
     2221 gaagcagaaa cgccatggcc tggaaatccg ccggcggatt gcggcggcgg ctccgaggaa
     2281 gccccgccat gcaggcgcta cggcacaata gccgttttca gatggccgtt gcgattcatc
     2341 atgtggcgaa gcactcccag aatgtcgctc aggggcgcct ccccggtgac aaaatcggcg
     2401 gcgcgaatct ggccctggga gatcacgtcg agcgcccgcc ggatatgcga gggcgtgtgg
     2461 tggaaactcg atttcagggt gatttccgag tagtgcagca gagcggtgtc caggctgact
     2521 tcgctgccgg tcgggcatcc gccgaacagg tttacggtcc cgccgctgcg caccatgcga
     2581 atcgcccact gccaggtctc cggctggccc acagcctcga tggccgcatc ggcgcctcgg
     2641 ccgcggtagg tgagccgggt caccgcctcc acgacattcg caaccgattc ggtcgaaatc
     2701 agctcatccg cgcccatgct cgctgcccgg tcgagttgcg tcttccgcct gccaatcgcg
     2761 atgacgcggg catccagggt cttggccagc cgcacgaaca taagtccgat cggccccagg
     2821 ccgatcacca ccaccgtgtc tccagggcgc aggtgagttt cgtcaagccc tctcatcacg
     2881 caagccagcg gttcgatcag cgccgcgtcc tgataaccga cgtgaggcgg aatttcatac
     2941 atattgcgcc gcacaatcgg gccggggatc cgtatgtatt ctgcgtaggc tccgttgttg
     3001 aacagcaggt cttcacaaag attctcctgg cggcgagcgc agaaataaca gttttcgcag
     3061 ggcgctgaat tggccgccac cacccgctgc ccgacctgga aattcttcac ctggcttccc
     3121 acttcgacta tgtcgccggc cagttcatgg ccgagcacag ccggcggaac gatcatgcgg
     3181 gcatgatagc cgcgccggaa aaccttcaga tcagttccgc aggtcagcgc gacgttcacc
     3241 ttcaccagaa cgtcggaggc tcccaaggtg ggaatctcca cgggttcgac ccgtaactct
     3301 tctttgccgt agaggacggc agccatcatt gtccgctctg tcttcacgtt catttacctc
     3361 tgtgggtgca cgatgatctt taaagtatgg ctgtcggggt gcgtggccat ttcgattccc
     3421 gagtggattt ggcccaagcg gaggcggtgt gaaaccaggc agtcaaccgg caaatgccca
     3481 tccagaacaa gccgggatga ctcatcctga agatcgacgt ccgcactata gcagcctagc
     3541 aaggtacgct cctgcgcgca aatactcgcg ccgtcaatct cgatcttttc cattgccgaa
     3601 gtctgtgcaa acaaaaggat tctggcgcct gggcgggaga ggcgcaccgc ctgctccgcc
     3661 agacccggcg cagccgcagc taccaggacc acgtcggccc cgcgcccgct gcttgcgctg
     3721 cgcactgcgt cggcagcttc ttcccctgaa gtctcgaaaa cccggaccgc ccccagcttc
     3781 cgcgccagct cacgccgccc ctccagaggg tcgatggcat gcacgatcgc cccccggcgc
     3841 acaagcagca tcgtgaacag caggccgatg ggtccctggc caagcacggc ggctatgtcg
     3901 cccgcctgaa catcgcattt tacaacggct ttgtaacaag tgttgaccgg ctccacaaag
     3961 cacgcccgat ccatgctgac gccgtcggga acgcggacca cgccccgccg gacgatccaa
     4021 tccatcactc gcacgtattg cgcgaagccg ccgcccgccg gctcgaaccc ggcggtgatg
     4081 ccgacgcgtt tgtaggtgct gcattgggca taaagacgcc ggcggcagta gaaacagtta
     4141 ccgcac
//
LOCUS       DASREK010000285         4211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2441369, whole genome shotgun sequence.
ACCESSION   DASREK010000285 DASREK010000000
VERSION     DASREK010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441369"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFA54_15390"
     CDS             complement(<1..385)
                     /locus_tag="VFA54_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HZS52251.1"
                     /translation="MWQQILPALRMGVFLTVVLGIVYPLAMTGISQVFFPYKANGSLI
                     ISGGRVIGSELIGQNFTRPEYFHPRPSSAGANGYDATASGGYNQGPTNRKLIERVAAA
                     VERFRRENPDYRGPIPADLVTGSASG"
     gene            complement(397..2523)
                     /gene="kdpB"
                     /locus_tag="VFA54_15395"
     CDS             complement(397..2523)
                     /gene="kdpB"
                     /locus_tag="VFA54_15395"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZS52252.1"
                     /translation="MATIATLPGPAPKASENDLNSLLPKNPAHAPPLFDREIVSRAIR
                     ASFVKLNPFTLSKNPVMFVVEAGAALTTLFLFRDLFAGGGNIAFQAQISIWLWFTVLF
                     ANFAEAMAEARGKAQADSLRKTKTDVMAKRLARGRVEHVSGSALRMGDIVVCEAGDLI
                     PGDGEVIEGIATVDESVITGESAPVIRESGGDRSAVTGGTKVLSDQIKIKITSNPGET
                     FLDRMIALVEGAQRQKTPNEVALNILIAGLTVIFLLAVVTLQPFAVFSAAQSQAGAIP
                     AVAVLVSLLVCLIPTTIGGLLSAIGIAGMDRVMQHNVLAMSGKAVEAAGDVNSLLLDK
                     TGTITLGNRQAVEFLPLNGVTREELADAAQLSSLADETPEGRSIVILAKEKYGLRGRH
                     IPEHEATFIPFTAYTRMSGVDFEGRQLRKGAADAIAKFAAQRGGEAPAEFQEMSDRIS
                     RSGGTPLGVADGSRLLGVIYLKDVVKGGMRERIAQLRRMGIRTVMITGDTPLTAAAIA
                     AEAGVDDFLAQATPKDKLEYIKKEQAAGRLVAMTGDGTNDAPALAQADVGLAMNTGTT
                     AAKEAGNMVDLDSNPTKLIEVVAIGKQLLITRGALTTFSIANDVAKYFAIIPAMFMTT
                     YPALAKLNVMGLHTPQSAVLAAVIFNALVIIALIPLALRGVKYRPMDAASLLRRNLLI
                     YGIGGIIAPFPGIWLIDRLLAALRLA"
     gene            complement(2553..>4211)
                     /gene="kdpA"
                     /locus_tag="VFA54_15400"
     CDS             complement(2553..>4211)
                     /gene="kdpA"
                     /locus_tag="VFA54_15400"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZS52253.1"
                     /translation="ALHPLLRWLEALTYKAAGIQEDAEQKWTHYTASLLSFSLFGFVM
                     LYLLQRMQGLLPLNPQRFAAANISPDLAFNTATSFVTNTNWQAYSGESTMSYFVQMAG
                     LAVQNFCSAAAGIAAAVALVRAFARQEKRTIGNFWVDVTRATVYVLLPLSIPAALFYV
                     SQGAVQNLRPYTEATTVEGGKQIIAQGPVASQEAIKQLGTNGGGFFNANSAHPFENPT
                     PVSNLLAMFLIFVIPGALTYTFGKMVGDTRQGWALFAAMAVMFLIGVLVCYGFEQEGN
                     PILARLGLQTEATSGQSGGNMEGKEVRFGVASSALFATATTDASCGAVNAMQDSFTPI
                     GGLVLLFNMMTGEVIFGGVGAGLYGILLYCILAVFIAGLMVGRTPEYLGKKIEQKEVK
                     MAMLAVVASAFSILFFTAVSSVIAFPEKSYWNPPGAAAANLNNSGPHGFAEILYAYTS
                     GTANNGSAFAGIAANTPWYNLTVGLAMWIGRFLFLIPLLAAAGSLAAKKRVAANAGTF
                     PTHGPLFVGLLVGTVFIVGALTFFPALSLGPIVEHYLMRSGSLF"
BASE COUNT          826 a         1297 c         1280 g          808 t
ORIGIN      
        1 ggccgctggc ggacccggtg accaaatcgg ccgggatggg tcctcgatag tccggattct
       61 ctctgcggaa tcgttcgacg gccgccgcga cacgctcaat cagttttctg ttcgtgggcc
      121 cttgattgta gccccccgag gctgtggcgt cgtatccgtt tgcccccgcg ctggagggac
      181 gggggtgaaa atactccggc cgcgtgaagt tctggccgat cagttcggag ccgatcaccc
      241 ggccccctga gatgatgagg ctgccgttgg ccttgtacgg gaagaaaacc tgcgagatgc
      301 ccgtcatcgc gagagggtag acgataccta gcactaccgt caaaaagact cccatccgaa
      361 gcgccggaag aatctgttgc cacataatct tgctccttaa gccagccgca gcgccgcgag
      421 caaccggtcg atcagccaaa tgccggggaa cggcgcgatg attccgccga ttccatagat
      481 cagcaggttt cgccgcaaga ggctcgcggc gtccatcggc cggtatttga cgccgcgcag
      541 cgccaacggt atcagcgcaa tgatgaccag ggcgttgaaa atcaccgccg ccagcacggc
      601 gctctgcggc gtgtgtaggc ccataacgtt cagcttcgcg agagcggggt aggtcgtcat
      661 gaacatcgcg ggaatgatgg cgaagtattt ggcgacgtca ttggcgatgg aaaacgtggt
      721 caaagccccg cgggtgatca gcaactgttt gccgatcgca acgacctcga tgagcttggt
      781 cggattgctg tcgaggtcca ccatattgcc ggcctctttg gcggccgtcg tgccggtgtt
      841 catcgccaag ccaacgtcag cctgggcaag cgcgggagcg tcattcgtgc cgtcgccggt
      901 catggccacc agccggcccg ccgcctgttc tttttttatg tattccagtt tgtccttggg
      961 ggtagcttgg gccagaaagt catccacgcc agcttccgcg gcgatggctg ccgctgtaag
     1021 gggagtgtcg ccggtgatca tgacagtgcg aatccccatg cgccgcagtt gtgcgatgcg
     1081 ctccctcatg ccccccttga ccacgtcttt cagataaatc actcccaaca ggcggctccc
     1141 gtcggcgacc cccaagggag ttccgccgct gcgcgaaatg cggtcgctca tttcctggaa
     1201 ctctgccggc gcttcgccgc cacgctgggc ggcgaacttc gcgatcgcgt ctgcggcgcc
     1261 cttgcgtagc tgccggcctt cgaaatcgac tccgctcatg cgggtgtagg ccgtgaatgg
     1321 gatgaatgtg gcctcgtgct cggggatatg gcggccgcgc agcccgtatt tttctttggc
     1381 gaggatgacg atcgagcggc cctccggagt ctcatccgca agactcgata actgcgccgc
     1441 gtcggccagc tcttcgcggg tgaccccgtt cagtggaaga aactccacag cctggcggtt
     1501 tcccagcgtg atggtgcccg tcttgtcgag cagcagcgag ttcacgtcgc cggcggcttc
     1561 caccgcttta ccgctcatgg ccagcacgtt atgttgcatc acgcgatcca tcccggcaat
     1621 gccgatcgct gaaagcaggc cgccgatcgt ggtgggaatg aggcagacaa gcaaagagac
     1681 aaggaccgcg acggcgggaa tcgcgccggc ttggctttgc gccgcgctga aaacggcgaa
     1741 cggctggagc gtgacgacgg ccagcagaaa gatcacggtc aagcccgcga tcaggatgtt
     1801 cagcgcgacc tcgttcggcg ttttctggcg ctgcgctcct tccaccaggg cgatcatgcg
     1861 atcgaggaac gtctcgcccg gattcgaggt gatcttgatt ttgatctggt cggacaacac
     1921 cttggtgccg ccggtgacgg cgctccggtc gccgccggac tcccgaatga cgggggcgct
     1981 ttcgccggtg atgacggatt cgtctactgt ggcgatgcct tcgatgacct ctccgtcgcc
     2041 gggaatcagg tcgccggctt cgcacaccac gatatcgccc atccgcagcg cggagcccga
     2101 aacgtgttca accctgccgc gcgcgaggcg cttcgccatg acatcggttt tggttttccg
     2161 gagactgtcg gcctgagcct tgccacgggc ctcggccatc gcttccgcga agttggcgaa
     2221 caggacggtg aaccaaagcc aaatcgagat ctgcgcctgg aatgcgatgt tgccgccccc
     2281 ggcaaataga tcgcggaaca gaaacagcgt ggtgagcgcc gcgccggcct cgacgacaaa
     2341 cattacgggg ttcttcgaaa gcgtaaacgg gttcagcttc acgaaggatg cccgaatcgc
     2401 gcggctcacg atctctcgat cgaatagcgg cggagcgtgg gccggattct ttggcagaag
     2461 cgagttgaga tcattctccg aagccttcgg agccgggcct gggagcgtag cgattgttgc
     2521 catcggtttt caactccaga ggcgcggctc ttctaaaaca aacttccgct gcgcatcaga
     2581 tagtgctcaa cgatagggcc cagcgagagg gccgggaaaa acgtcaacgc gccgacgatg
     2641 aacactgtgc ccaccagcag tccgacaaac aagggcccat gcgtggggaa agtgccggcg
     2701 ttggccgcga ccctcttttt ggctgccagg ctgccggccg ccgccagcag agggatcagg
     2761 aatagaaacc gtccgatcca catcgccagc cccacggtca ggttgtacca aggcgtgttc
     2821 gccgcgatgc cggcaaaggc gcttccgttg ttggccgttc cgctcgtata cgcgtacagg
     2881 atctcggcga acccatgcgg gccgctattg ttcaagttcg ccgccgcggc gccaggcggg
     2941 ttccaatagc ttttctcggg aaacgcaatc accgaactga ctgccgtgaa gaacagaatg
     3001 ctaaaagcgc tggcaacgac ggcgagcatc gccatcttca cttccttctg ctcgatcttt
     3061 ttgccgagat actccggcgt gcggcccacc atcaggcccg caatgaaaac agccagaatg
     3121 cagtacaaca ggatgccata gagcccggcg ccaactccgc caaagatcac ttcgcccgtc
     3181 atcatgttga acagaagcac cagccccccg atgggcgtga agctgtcctg catggcgttg
     3241 acggcgccgc agcttgcgtc cgtcgtagcc gtggcaaata acgcggaact tgcgacacca
     3301 aaccgcactt ccttgccctc catgttgcct cccgattgtc cggaggtcgc ctcggtttgc
     3361 agtcccagtc tcgccagaat cgggttgcct tcctgttcga atccatagca aaccaaaacg
     3421 cctatcagga acatgacggc catcgcggcg aacagggccc agccttgccg cgtgtcgccc
     3481 accattttgc cgaacgtgta cgtaagtgcg ccgggaataa caaagatcag gaacatcgcc
     3541 agcagattgc tgacaggagt cggattctcg aacggatgcg ccgaatttgc gttgaagaac
     3601 ccgccgccgt tggtccctag ctgcttgatg gcttcctgcg aagcaacggg cccctgggcg
     3661 atgatctgtt ttcccccctc cacggtcgtc gcctcggtgt atggtctaag gttctgaacc
     3721 gcgccctggg acacatagaa caacgcagcg ggaatcgaga gcggcagcag cacgtacacc
     3781 gtggcgcgcg tcacatcaac ccagaagttg ccaatggtcc tcttttcctg gcgcgcaaaa
     3841 gcccgaacca gcgcgaccgc cgcagcaatg ccggcggcgg cggagcaaaa gttttgaacg
     3901 gcgagaccgg ccatctgcac aaaatagctc atggtggact cgccggaata ggcctgccaa
     3961 ttcgtgttgg tgacgaagct cgttgccgtg ttgaacgcca gatcggggct gatgttggct
     4021 gcggcgaaac gctgcgggtt aagcggcaga agtccctgca tccgctgaag caggtacagc
     4081 atcacgaacc caaacaggct gaagctcagt agagacgcgg tatagtgggt ccatttctgt
     4141 tccgcgtctt cctggatgcc ggcggccttg taggtaagcg cttcgagcca ccggagcaac
     4201 gggtggagag c
//
LOCUS       DASREK010000286        35341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_476327, whole genome shotgun sequence.
ACCESSION   DASREK010000286 DASREK010000000
VERSION     DASREK010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 35341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35341
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476327"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2955
                     /locus_tag="VFA54_15405"
     CDS             <1..2955
                     /locus_tag="VFA54_15405"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZS52254.1"
                     /translation="FRIDGNLHGFDLRYLARRYATPQFVWDGAVSGPLHAASRLRGSL
                     SRSLRASAELAIAPRHQGVPLAGRIIVSYDGPRDAIDLGNSFVVLPSSRLDFSGSIGN
                     HLSIRLVSRNLDDFLPALSLTSENPPRALPVTLLNGAARASATVTGKLSDPRIAGRIE
                     LTNFAVNRRKFDHFSAEWNAAGTGAAVRNAVLRRGALLAELSGSAGLRRWKPEPSEPV
                     AAAVAIRNAQLQDLLALANQASLPVSGVIAAEGNVSGTLGDPRVVVAVSLTNGAAYGE
                     PFHRIQALASYSRQWVQLISAEASAPAGSIHFSGAFEHPPDNFRQGRVQFQIGSTRIE
                     LQKLGIVQKKDPRLTGTLELNAEGAGALQAGSTATPFLFSSLSAKVSAHVAEVNTNRA
                     GNLLLTADTQGQNLNFHLQSDVASSQIRGDGSLVMRDDYPLQTKITFSAVDIAALARM
                     FGKPGQPANQTFSGALEGAASLAGPMRRPQNIKGRLEITKLQVNALTPAAPGAAAVRP
                     LAFHNPSPIVATLDRSVVHIESARLTGPSTDFSVSGAVYLNKQNALDLRASGNVDLRL
                     AQTFSPGVFSSGNVQLEAAVRGTASKPALNGRLQLQKASFNMVDVPNGISNANGVVLF
                     NSNEAIIQTLTGETGGGKVSFGGTVRYGGPEMAFNLQARAQQIRVLYPSNVSTQLSAN
                     LTLNGTTTDNLLSGSVIVQDVAFYSHTDLGRILSGAAAPPRAPAAQTGVLGGMRFDVR
                     IDTSSAFQLQTALTQNVQAEAHLQLRGSPSSPGMLGRIDAQQGEVIFFGSKYSITAGT
                     ISFFDPNNIEPVVNFALETQAQGITVDIMVRGPMDKLTMTYRSDPPMQFSDIVSLLAT
                     GRVPSTDPVLAAQQPAAPQQSMQQFGASAVLSQAVANPVSGRLQRLFGVTKLKIDPQI
                     VGPENTPQARLTLEQQITKDLDFTYITDLTQGNQQIVRIEWAIDPTWSAVAVRQEDGQ
                     FGVDIYYKKRFR"
     gene            complement(3022..5112)
                     /locus_tag="VFA54_15410"
     CDS             complement(3022..5112)
                     /locus_tag="VFA54_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HZS52255.1"
                     /translation="MSRILFLAAAFCGFILAAAPGETAALPLMPMPARLSMGSGSLTI
                     DSRFSIAISGYSDQRLEAAAMRLLARISRQTGVPIPRQIGKGGSPAALVIACKSAGSA
                     PPKLYEDESYQLEIAASGARLSAPTVTGALRGMATFVQLIAPGPDAFTVPAVRIEDKP
                     RFPWRGLMIDSSRHWMPLPVILRNLEAMAAVKLNVLHWHLSDDQGFRVESKIYPKLQQ
                     EGSDGLFYTQDQIRRVVAFARDRGIRVVPEFDMPGHSLSWLVGYPELASAPGPYEIGR
                     HWGIFDAVMDPTREETYNFLDSFIGEMAGLFPDEYFHIGGDEVRANQWNQNTAIQAFC
                     KAHNLAGAHDLQAYFNKRIQAIVQKHGKIMIGWDEILHPDLPKDIVVQSWRGQESLAQ
                     GARRGYRGLLSFGYYLDHLSPASYHYGIDPLGDGAQELNAEEAARILGGEACMWAELV
                     NAETVDSRIWPRMAAIAERLWSPAELRDVDSMYARLSLVSRGLDWTGVQHRSTYWPML
                     DRLTGGRSTGQLKVLSDVVEPQGLRGRHPREKTTSTPLNRLVDAARAESESVRALSQA
                     ARKIVEEPGNSAAERAWVRQTFLIWRDNDARLQPLLQNNFLLKETAPLSKDLSRAGTI
                     GLQALRYVESKRPAPARWVSQQKQALDRMEKPQAEVTLAAVRPVRILVEAVSRTRASG
                     QSRRRPAPRKAPQK"
     gene            5714..6868
                     /locus_tag="VFA54_15415"
     CDS             5714..6868
                     /locus_tag="VFA54_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS52256.1"
                     /translation="MDTLARHAERVESLLEVLGSGAWVADLLTDVLTHSEIYGTHRSD
                     AEWLKERITGAYLFPRGGAVCPGENGPGRRPEFQPVDRQWVHSLLRTLESNTWVAGFT
                     ADVLDRCAADGVGACTPEWIQMRLTQATDFAARAGTFNQTPGTRGTTVLNIHQFAEPG
                     NQAAYAFREMPSVIAVANLKGGCGKSTIAVNLACELASKGNAVVLVDTDSQGTASCWI
                     ASGQLPIQGESMPLETAEDAELLTNKILASNAQYVVVDAPAHVVAATRAAGGLADLVL
                     VPVTASGVDLQATAGAMDLIHKVRASRGNRGPKCLIVPSKVDRRTGAGREIEAALQRF
                     GEAIGPAVHQRAAFADAFSAGKWVGQYAPDSPAHYDIASLTVAVKRTLRT"
     gene            6865..7371
                     /locus_tag="VFA54_15420"
     CDS             6865..7371
                     /locus_tag="VFA54_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52257.1"
                     /translation="MNKNSQNGHASASGKVNGLPPVWMSLPCPNLMKVIIAAADEKMA
                     PVAIEGWFEPEFQPAVPPVREVRARGGSEVIPSPPPAAAGDAPGPPVPLPDVFRDLVQ
                     ARSRSPWAARRWIIPAVVVLAILFLLLGAKMVRSRRLNPANAGIERALSGEYGSSKQD
                     RVGQQALP"
     gene            7325..8974
                     /locus_tag="VFA54_15425"
     CDS             7325..8974
                     /locus_tag="VFA54_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52258.1"
                     /translation="MDQANKIELANKHCRELSQEVQEGKAKISESLACAQVLQIEGKT
                     DEALQELYRARDRSEHFVERLRAAAQMQIELKQYEAAAGAYREILQLDDKIRTACFGL
                     ALCLEKLGRWEQANKKVQRALDVDPRRAEAQIGLATCLLHLGQTGEALKIFDACLQRE
                     PDCEGALLGKAVALQLDQRLEEAAELYQRVLSLNPNSQDALANMIAIGIAKRAYQIVR
                     DCSSRLLAIQPRSRTALEGLAISAFSTRDYAAAAKYCGDLVQIEPHSFANWFNLGVAQ
                     QKLGDAPAAARAYCEALRLDSRKPQAYLNLGCAQQEMGQIEAARSSYENALRLAPDLE
                     PALWNLGLLSEVIQDSERAETVYSRLVERNPGHEEAWFRLACVRLARGDFGRSIEAFQ
                     ECLKKRSKWHEALLNMGIAYWRMGDPDSAQEYLHEALACRPKSRHTLGVLAALAIERE
                     DFDEALELHGRLVDLGERNPEVLYNAGFLFQRAGKLEDAARAYEKALAERPKFREALL
                     NLGHSYRSLGRDEEAQAMWLKAVEAASPAAGSGQPALDRPQ"
     gene            8984..12748
                     /locus_tag="VFA54_15430"
     CDS             8984..12748
                     /locus_tag="VFA54_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZS52259.1"
                     /translation="MKLAKQLGRYQIQAEIGRSAIASVFRALDPPLDRLVALRIPVDG
                     AEGLFRGLADQAAAAAKWHHPGIVTVYELGEHGGVPFIVLEYLSGKTLRQLIADRSPL
                     TVLEKTLLMSQVADALRFAHQSGAIRLDIRPSKIMLMPDGSARVLDFLPHGWRDFCRS
                     PEDRALYAAPECGASPGDIRSDIFVFGVVCYELLTGLHPFPDEEAARAGVLMPASSLC
                     PDCPESLEQIVQRTLRQDPELRYQTPWDLKLELDGVLIELKRERAASLVAESERLAAQ
                     NEFETAQALLNEALALDPGNRNARLLRETARKQAQQHLLEQAKEEIAERRFASAIQML
                     ESALRIDASQGAIRERIAEARALLERSEAADKLIEQALRKFEQDKLPEARKIAVEAVE
                     TDPLSPQAAELLQLIETEIAGREARLEQSLADAEELLRAEQFDEAIALLRSLDAEYPN
                     SGKIKRRLAQARSKKADRKRRQRLQAKLAPVESLMAERRFAEAVERLQALLKEFGKEK
                     QLFEWLLQAQKELEREQRRLALENARSQVTAFCQAGDFGAAGAHVLGTLQSYPNEPEL
                     MQLLEEVTASQKAWEEGVARGVEEAEWLMNQGRPDLALPLLERDVGTYPGSGRLLDLL
                     AAAKQALPEYERRQFTQHAIARSSDLEARGQTHAALALLGEALTRYPKSEELAAAARQ
                     MRARRDQAERQQRIAQRLEAIERKIAEQAWAQALVLAQAARSEFPGDSDLERVLQRAE
                     DGKRRAEREDLLAGIEQSVLEADIDRALAKIDDALQKNGTDPELENRRQELQREKQYR
                     ESLREAEVLFGRRQFQEAENAVRQAAALKPCENSARVLLEAIRAERAAEEEAEFYSRG
                     REKAARLIRERQFHQALDLLHNLLSLFPGDPILERDLRAAESARNDSSAAAIAELPVE
                     DPDVEKADLLPESPPAHDPAPSGLCAVPEAADPDAGVGDVHPRATDNPALPEVPVPSA
                     AVAPPPVAPSPQPQFAISSGPSWKRPAFVALPAVFLAAAAVLPIWISSRKAPQAASAR
                     GTVTTPAGSQPAPRPGMPERSGNFRDDGRASNIPERPVETNEGVAHSKAAERLPAERQ
                     EPAPRLPRKFDISRLRRGASEQSKLTPEEPLSGISVPDPGNLPAAVFEPSLAKPAAPP
                     QDAPMATSKAAVERIRTGGNFQQPAFISGPAPVIPPAARERGIYGVVKMELAINKQGI
                     VTEARLLSGPAILVPAAREAALQRRYRPATLDGEPVEVKLPIQFVFQASR"
     gene            13109..13573
                     /gene="msrB"
                     /locus_tag="VFA54_15435"
     CDS             13109..13573
                     /gene="msrB"
                     /locus_tag="VFA54_15435"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZS52260.1"
                     /translation="MSVSIVEFTDAGERKGVVTVDKIVKTDAEWQKQLGTGRFFIARK
                     KDTEPAFSGEYWNLHEDGIYRCACCGTALFDSATKFESGTGWPSFWAPIAEENIQTET
                     DTSFFMVRTEVLCRRCDAHLGHVFEDGPPPTGLRYCINSAALRFVKGPDASS"
     gene            complement(13596..14807)
                     /locus_tag="VFA54_15440"
     CDS             complement(13596..14807)
                     /locus_tag="VFA54_15440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZS52261.1"
                     /translation="MTRRNALRNLAVWLAGSPLWRPPLEAQQLPGESDRVPLLDDLVN
                     VLEFEPVAKAKIPKANYDFIAGGVDDEWTLRRNREAFNWISLRPHMLRDVSKLDLSLT
                     LFGQKIETPILVAPTGTQALAHPQAELAMAKAAGAAKTIMVVSTNSSFPIEKIAAAAT
                     GPLWFQLYPGPDHPGTRERVQRALAAGCKVIDITLDTPYVSHRERLLRNRQEQEVAPG
                     DALPQLVDRGEQSSVRRRAAAPRPYRLQKRFTAELTWDFVQEVNSYAKVPVLVKGILT
                     PEDAKAAIEHGAAGIHVSNHGGRYLDYDPSTIEVLPSIIDAVGGRVPVIVDSGFRRGT
                     DIIKALAVGAKAVLVGRPPLWGLAAYGQAGAQRVLELLQSELALAMGLSGRPNLESID
                     RTLIEIHPPRA"
     gene            complement(14922..15416)
                     /locus_tag="VFA54_15445"
     CDS             complement(14922..15416)
                     /locus_tag="VFA54_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZS52262.1"
                     /translation="MRRRWIVLTLIACAACLPMARSECEAPAPVQPLPFSHKQHMALR
                     LQCKRCHEMPGQGWEAGLPATQTCMSCHTRVKRDSPFIQKLAEFHKQAKPVPWARVYS
                     LPAWVDFSHKEHVETGNETCEACHGPVRERDTLCVETDISMRGCLECHVEKNAPRGCR
                     FCHL"
     gene            15614..16282
                     /gene="thiE"
                     /locus_tag="VFA54_15450"
     CDS             15614..16282
                     /gene="thiE"
                     /locus_tag="VFA54_15450"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZS52263.1"
                     /translation="MQNELRANRAGKFRAAIFRGLYPIIDTAACARAQIQPESLARAI
                     ARAGVSMAQFRHKGAFTREIFAQAENVGQILQAAGVAYVINDRADVALLLGADGLHLG
                     QGDLPPARVRPVVGSSLAIGYSTHNRYQLLAGDREPVEYLAIGPIFATGSKENADPLV
                     GVEELARLRRLTAKPLVAIGGITRANARAVLDAGADAVAVISDLLAADLEARLNEWLI
                     LTQR"
     gene            16258..17664
                     /locus_tag="VFA54_15455"
     CDS             16258..17664
                     /locus_tag="VFA54_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS52264.1"
                     /translation="MADTDAAVTSTTRSPGLIQGLGLIDATTLVMGSMIGSGIFIVSA
                     DISRQVGSPGLLILVWLLAALLTLIAALSYGELAAAMPQAGGQYVYLREALGPLSGFL
                     YGWTLFLVIQTGTIAAVAVAFAKFSGVFFPWISAANPVIAVGRFGISTQQLVAIGVLI
                     FLSLVNSFGIRTGAAVQNVFTFAKTAALLGLVLLGFLLGSNALAVEQNFTAFWRGAGW
                     SFEVFRAVAVAMVGALFSADAWNNVTFTAGEVKNPGRNLPLSLGLGAGIVLALYVATN
                     FVYLRWLPLSPGIQQAPEDRVATAAVQLMIGRGGERLMALAIMVSTFGCMNGLILAGA
                     RVYYAMARDGLFFRRVAEIHPRYHTPAVSLAVQCAWACLLTLSGTYSDLLDYVIFAVL
                     LFYILTIWGLFVLRQTRPEMPRPYRAIGYPWAPALYILLAALIEVMLLIYKPGYTWPG
                     LIIVLLGVPVYHLWRRRA"
     gene            17731..19203
                     /locus_tag="VFA54_15460"
     CDS             17731..19203
                     /locus_tag="VFA54_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS52265.1"
                     /translation="MADLLIQKPLNLLMREANEEGAHTLKRVLGPWQLTALGIGAIIG
                     AGIFVLTGEAAYQNAGPAVVLSFIASAIACGFAGLCYAEFASLIPIAGSAYTYGYATL
                     GELFAWIIGWDLILEYALGASTVSVGWSGYFISLLNYFGIQLPASISTSTFIVNAQGR
                     FENTGAFNFIAFGILLVISAILAIGIKESATLNAIIVFLKVAIVLLFIAAGAPYVNPQ
                     NWHPFIPPNDGVFGHYGWSGILRGGALVFFAYIGFDAVSTAAQECKDPKRDLPIGILG
                     SLGICTLLYIAVSAVLTGLVSYKLLGVPHPIAVGIEATPFRWMAWVVELGAVLGLGSV
                     MLVMLLGQSRVFYTMSRDGLLPEVFSRIHPRFRTPYVITILTGAVVAIVAGLLPIRVL
                     GEMVNIGTLLAFAIVSASVIVLRVKRPDLPRPFKTPGYPFVPVLGVLTSLALIGFLPE
                     DTWWRLIIWLIIGLVIYGFYGIRHSKVRAAAGGRVPVPGD"
     gene            19334..21388
                     /locus_tag="VFA54_15465"
     CDS             19334..21388
                     /locus_tag="VFA54_15465"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZS52266.1"
                     /translation="MASRLLQQRLPGASLLPFAVFLAAIALLIKLARAGSRKLLWRVR
                     NRMILVFLFIGFVPLLLVTALAGLATYILAGQVAVYIASTELDRRAERLRDTAAALAW
                     SLEATAPARRPELAQRYLSRAAQAWPELQAWIRDSTGTTVFPAGSSVEPPRSEARGIV
                     RRGDGFYLVARPSGSSPVVGGEPADSANVPPPEPEAASGNVKRKDPLDVVLLQPLDPV
                     NLAALAPGLGSIEFLPWRRVLMRRPNGAAAEVNLLPERRVRPAAFEGGEPLPAPANRF
                     DYEVLWGTSLFEVGRWSDRDRPTLVNLLVRTRPSAVYRVLFGRRVEVASTVTVFSIII
                     GTLFLMVELASVATGVSITRTITNSVHQLYEGTRRVNRGDFSHRIPADGRDQLGELSR
                     SFNNMTESIERLIEESKERQRLASELEIAREVQQQLFPKAPPSMRQLEILGVCNAART
                     VSGDYFDFIKISDDHLALALGDVAGKGISGALLMASMQSMLRTQLAVAAYFGTIFRRD
                     PAMFTALSGELSGSAANDAGWRFPTDKVVSQLNRQLYENTPPEKYATFFLGAYDDCRG
                     LLAYTNAGHLPPVLIRKGQPSRLDVNGMVIGLFPWAQYERSEIELQPGDLLVAFTDGV
                     TEPENEFAEEFGDERLIDLLVRNSDRPLREIIQEVTAAVGEWTGRPELQDDMTMVVAR
                     RL"
     gene            21494..23122
                     /locus_tag="VFA54_15470"
     CDS             21494..23122
                     /locus_tag="VFA54_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZS52267.1"
                     /translation="MKVLTAAQMREIDRRTSAERGVPSLVLMENAGARVFEFLAHRFS
                     PLSRERICVLCGKGNNGGDGLVVARQLLTRGRPGRLKVILAAHPDSLLGDAAANYRMF
                     LGVDGQVRAVTSEAEWESALGEILDSTLVVDALLGTGLRGPAEGLMLRIIQDVNQRLR
                     HASVVAVDIPSGLASDTGEALGESVRAGYTVTFTAPKVGQIFPPNCEAVGQLYVAPIG
                     SAPSLYENDSEIFLSTIDSEYVAPLFAPRARGAHKGDFGHVLVVAGSTPKPGAAILAG
                     TAALRAGAGLVTVATTARAAASITAHTPELMTEPLPETEMGTVSLAAFDYQRFEDILA
                     RKNVVAMGPGLGTNDETRAFLRKAVDLCAPGNRRQPVRLVLDADALVPDNVRPGVVMT
                     PHPGEMARLTGLDASYIQKRRVETAREFACSRGVFLVLKGYRTLLATPEGRVLVNLTG
                     TPAMASGGTGDVLTGLVAGFLSEFPQAPMEQAIAAAVYLHGRAGQLAAQELGEQAALA
                     TDVLRHLSRAIRELTETSCGQPPAILPQPLYDSP"
     gene            23138..23560
                     /gene="tsaE"
                     /locus_tag="VFA54_15475"
     CDS             23138..23560
                     /gene="tsaE"
                     /locus_tag="VFA54_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZS52268.1"
                     /translation="MPETFFTHSEEETIAAGREIAKRLRPPALVLLIGELGAGKTTLA
                     KGIVSGLGAAREEDVSSPTFTLIHEYGKDPKVFHIDLYRLDRPPELETLGLDDLWDQN
                     AIVLIEWGEKFAAQLPREHLEIRLEYAGDDERRIAVAE"
     gene            23612..24418
                     /locus_tag="VFA54_15480"
     CDS             23612..24418
                     /locus_tag="VFA54_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS52269.1"
                     /translation="MATELQPWALILGSSSGFGEATAIELARSGMDIFGVHFDRRATM
                     PHVEEIQSKIRALGREPVFFNANAVDEQKRKEIIEAIRQRCCNPASPGTVRVLLHSIA
                     FGALLPFVSPDAAEKTATKAQLEMTADTMAHSLVYWVQDLVRAGLMTRGGRIYAMTSA
                     GGTRAIPQYGPVSGAKALLESHIRQLALELAPFGITANAIRAGVTDTPSSRKIPGSDK
                     LFEFAAMRNPHGRLTTPEDVAKCIAVLAHENTYWMTGNTIGVDGGEDIVA"
     gene            complement(24524..27790)
                     /locus_tag="VFA54_15485"
     CDS             complement(24524..27790)
                     /locus_tag="VFA54_15485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZS52270.1"
                     /translation="MRKRLAGAGALRWIVWCLWAGSIAYAQVNTADILGTVTDAAGAV
                     VPTAKVTVENLATHEIRTTQSSGSGDYVVNLLQPGQYTVSVEAPSFKKATINLTLTAG
                     DRARADVQLQVGEVTQVVEVAAQSPALQTDSATLRTVVTEQAVQDLPLNGRNYITLVQ
                     STVGVGVGPSNSILSGTRPDERRQTANVTANGQNEVFNNQLIDGMDNNEREQFTILMR
                     PSIDMISEVKVETNAYSAELGRAGGAVINLITKSGTNEYHGGAYEYFRNDKLNANDFF
                     SNAGGTPRPDFHQNQFGGSFGGPIRRNKTFFFADAEALRIVQGQPTGLLSVPTRFEEL
                     NPGNFSDQGGPVLPPSQLDPVALNYWKLWPAPNVPGAAGIQANYSGNVKKVYNSTTYD
                     ARLDHHFTDSDSFFARFSYNPTFNSQPALFPDVNGISAGSGIFPGPSYADSQGYMMDY
                     VHIFSPTLVMEAKAGFIRLNLQTLSPNQGTNASQKFGMPNADVSQQISGLATIQVSGF
                     QAPGSSFTLGDDRFVPIYDINNVFQYQGSLTWTRGAHNIKFGGGVIRRQLNYYQNTFG
                     LGYFQFLSDPLTNLENLLLGKPTQIQRQVNFYKQYFRFWEPSVYTQDDWRVNRWLTLN
                     LGLRWDYFSPITSATGQRANFDLGTLTMKVGSNPGPVQPVYTNFEPRFGFAATTRRAI
                     VIRGGFGMSRFPQDYASGALNLLNAPFVSTNFNCQPAATSGALVCPPGIGTLAAGPPL
                     GVIPPLNNLIPGVLGAHDTHYPQAYIMQYNLTFQKQFGQNVVSVGYVGELARHLQYAP
                     NENLPDPSPGALNPFYYSSLLPNVSTINHYTATGAAEYNAMELVFERRYSKGITVNAN
                     YTFARNLTNISDGGTTGAATVGGILPRNRSYDWGNSDIGIKHRFSFRANYELPFGKSV
                     NGWRKFVYGGWQANTLAFWQSGTPFTVLDAAFNPAPTNVTSVTTDRPNTVPGQSYVPA
                     NQSITNWINLNAFTIQPKGTPGNEARTQLYGPTQRSVDFSLFKDFPFRERMKLQFRAE
                     VYNISNTENFGQPNITITKWNGTGPGATPTQAGNFGQIIQSNLALNPRQFQFALKLVF
                     "
     gene            complement(28284..30593)
                     /locus_tag="VFA54_15490"
     CDS             complement(28284..30593)
                     /locus_tag="VFA54_15490"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZS52271.1"
                     /translation="MSFRKQDALAYHASPSPGKISITPTKPCLTQLDLSLAYTPGVAE
                     PCLEIARDPQLGWRYTSRSNLVAVVTNGTAVLGLGNIGPLAGKPVMEGKAVLFKRFAD
                     IDVFDLEVNSQNPDDVIHVVQLLELSFGGINLEDIKAPECFYIEETLRRTLKIPVFHD
                     DQHGTAIISGAALVNALALTGKKIDQVRVVFNGAGAAGVSCADLYVRLGVRPENILMC
                     DSEGVIYQGRTKGMNPYKERFARDTNLRTLEQAMKGADVFVGVSIANCVTQDMLRSMA
                     DRPIVFAMANPDPEISYNDAIAARKDVIMATGRSDYPNQVNNVLGFPFIFRGALDVQA
                     IAINDEMKLAAAYALAEIARQDVPDAVRRVYQNERLEFGPGYILPKPFDSRVLETVPV
                     AVARAAMESGVAQKKIDLEEYRESLARRLGKAQEVARVMIQRAKQAPKRIVFPEGETE
                     KILRAAQILADEKIAHPILLGDHERILSLARKLGLQNSSWETLDPSRSPAAADYAREL
                     HSLRHRKGVTMDEAEAMLRDPIAFGAMMVRRGDADGMVAGITRHYLETIRPALQIINL
                     RPGVNRVSGLYALIINRKIYLLADTTVNIEPSSEDLAEITLLAAERARRFGVEPRAAL
                     LSFSNFGSVRHPLSEKVRRAVEILHERAPELIVDGEMQADTALNPDLIERFFSFSRLR
                     SEANILIFPCLEAANIAYKLLTRLAGAEAIGPILMGLSRPVAVLQKGFDVQDVVTMAT
                     IAVLDAQETSRPSRQTELAYAQAHRSGDH"
     gene            30628..31176
                     /locus_tag="VFA54_15495"
     CDS             30628..31176
                     /locus_tag="VFA54_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52272.1"
                     /translation="MRPATLPRARADPPPAPARKQLLLLCYPAGVEIWEVFQSLGRPA
                     LDELVRHISLGTLKAYKLYEPFKIRAHLTKLNTEHLRKSTGRFWDRLSSGDEGLAKDL
                     AQAILVSNIGFIVEVLNFLNIPHDGNGFLPKDFSGEKYLTEGWQQRVENEFRGRYPDS
                     LLRLYINHLMWEVDKQAKIYAS"
     gene            31277..32449
                     /locus_tag="VFA54_15500"
     CDS             31277..32449
                     /locus_tag="VFA54_15500"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZS52273.1"
                     /translation="MAKKRIGILTGGGDVPGLNSVIKAVVYRGGEIGFDVLGIRKGWE
                     GLTHVNLSSGIDDRYVLPLNRANTRTIDRTGGTFLHSSRTNPAKMKVAKLPPHLAQKA
                     GAFREVKPGVVDVTPAVLENIQALGIDALVVIGGDDTLSYAPVLEQHGVPVIAVPKTM
                     DNDVQNTEYCIGFSTAVTRAVAAISRQRTTVGSHERIGIFRIFGRDAGYTALYTAYVT
                     SIRCCIPEFRFDLAKLIDLLMTDKRNNPSNYALVVISEGATWQGGEVKEYGEADAFGH
                     RKKVNIGEALSDEIKKATGEETIVSDLTYDLRSGEPDFVDKLVAITFANMALDCLAEG
                     KHGRMMAIQNGCYVDTNIPDSSLGPRKVDVSTMYNCDRYRPNYTNKNGWPIFLTRV"
     gene            32751..33668
                     /locus_tag="VFA54_15505"
     CDS             32751..33668
                     /locus_tag="VFA54_15505"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HZS52274.1"
                     /translation="MGPIAEGGSRRMSTGMFGPAPSVSAEQIEAALANLRQLPASEPV
                     VHRALAQLDGCDFIVAELKETLLSDAALAARILRLANSAFFGFRSEVRTMSQAIVLLG
                     QNRLRTLLHRILADKIWTALGQERIAAAPLRTMSLATATAACTLSQLLLRENAEEMLL
                     AGLLHNIGELFLLSQFQAEYLRMIALAGELGLRRAAAQVFGPPPEICGKLLLEIWGFP
                     RLYSAIVEHMDCPLDARRPAEYASAIVLVHAARRMAEPFVAGSDAAEAIRAVPSEVCD
                     ELGLQPDLLAEVFQTLPHRMSLEQLQAAR"
     gene            complement(33687..34760)
                     /locus_tag="VFA54_15510"
     CDS             complement(33687..34760)
                     /locus_tag="VFA54_15510"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZS52275.1"
                     /translation="MPTFKIAVIAGDGVGQEVIPEGIRVLNAAGGRYDAAFEYTHHDW
                     GSDYYFRHGRMMPVAALDVLRPADAIFLGAIGHPDIPDHTTLNGLLLPIRRTFDQFAC
                     VRPAYLYPGVSSPLAGKAPGSIDFVVVRENTEGEYSQVGGFVYHHMPEEVAIQTAVFT
                     RRGTERVIRYAFDLARRRNKKKRVCSVTKSNAQGFGMVLWDRTFTDVARDYPDISTES
                     LLVDAAAMNFIRRPESFDVVVASNLFGDILTDIAAIITGSMGLAASANINPRRDCPSM
                     FEPVHGSAPDIAGKGVVNPLATILSAAMMLEFLGLQDAAQAIDRAVAAVLAEGRNIPA
                     DLGGSASTRQVTDAVLHHLSLQG"
     gene            complement(34860..>35341)
                     /locus_tag="VFA54_15515"
     CDS             complement(34860..>35341)
                     /locus_tag="VFA54_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS52276.1"
                     /translation="INPAITHGVAHIIGSIEPGKLADLVLWKPAFFGVKPEIIVKGGF
                     IAWSTMGDANASIPTPQPVLYRPMFGAFGEAAANTGLTFVSKAALEAGVPKTIGLSKK
                     AVAVRGCRNITKAEMVHNHATPHIEVDPETYEVRVDGELVTCEPAAVLPMAQRYFLF"
BASE COUNT         6652 a        10605 c        11103 g         6981 t
ORIGIN      
        1 ttccggatcg acggaaactt gcatggcttt gatctgcgct atctggcgcg ccggtacgcc
       61 actccccaat tcgtctggga cggcgctgtt tcggggcccc tgcacgcggc gtccagattg
      121 cgcggttcgc tctcgcgcag cctgcgcgct tccgccgagc tagctattgc gccgcgtcac
      181 cagggcgtgc cgctggcggg aagaatcatt gtgagctatg acggcccgcg cgacgccatt
      241 gatttgggaa attcctttgt cgtgctgccg tcgtctcgcc tggacttctc cggatcgatc
      301 ggaaaccatc tgagcatccg tttggtttcc agaaatctgg atgattttct gccggcgtta
      361 tccctgacat ctgagaatcc tcctcgggcg cttccggtca cgctcctcaa cggcgccgcc
      421 cgggcgagcg cgacggtgac cgggaagctg agcgatcccc ggattgccgg gcgtattgaa
      481 ctgacaaact tcgccgtcaa ccggaggaaa ttcgatcatt tttccgcgga atggaatgcc
      541 gccggcacgg gagcggcggt acgcaacgcg gttttgcgcc gcggcgcgct cctggcggag
      601 ttgagcggat ccgccggctt gcgccgatgg aaaccagaac ccagcgagcc agtcgcagcc
      661 gccgtggcga tccgcaacgc acagcttcag gatctgctgg ccctggcaaa ccaggcatcc
      721 ttgccggtct ccggcgtgat agctgctgaa ggaaacgttt caggaactct gggggatcct
      781 cgcgttgtgg tagcagtaag tctcaccaac ggcgcggcct atggcgagcc cttccaccgc
      841 attcaggcgc tagcgagcta ttcccgccag tgggtgcaat tgatttctgc ggaggcgtcg
      901 gcgcccgccg gcagcatcca tttcagcggc gcttttgagc atccccccga caattttcgc
      961 caagggcgtg tgcaatttca gattggcagc acccgcattg agctccagaa gctagggata
     1021 gtgcagaaga aggatccccg gttaaccggc acattggagt tgaacgcgga gggcgcgggc
     1081 gccctccagg ccggttccac cgccacaccc tttctgttct cgagcctgag cgcgaaagtc
     1141 tccgcgcacg tggctgaagt taacaccaat cgggccggaa acctgctgct gaccgcagat
     1201 acgcagggcc aaaatctcaa ctttcatctg caatccgacg tagccagctc gcagatccgc
     1261 ggcgacggct cgctggtgat gagggatgat tatcccctgc aaaccaagat cacattttcg
     1321 gcggtcgata tcgccgccct ggcccgcatg ttcggcaaac ctggtcagcc cgcaaaccag
     1381 actttcagcg gcgcactgga gggtgcggcg tccctggccg gtccgatgcg ccgtccccaa
     1441 aacatcaaag gccggctgga aatcacgaag ctgcaagtga acgcgttgac gccggcagcg
     1501 ccgggcgcag ccgccgtacg gcccctagcg tttcataatc cttcgccgat cgtcgctaca
     1561 ttggaccgct cggtggtgca tattgagagc gccaggctga caggtccctc gaccgacttc
     1621 agcgtgagcg gagccgttta cctgaacaaa caaaatgcgc tggaccttcg agccagcgga
     1681 aacgtcgatc tcaggctggc gcagaccttc tcccccggcg tcttcagcag cggaaatgtt
     1741 caactcgagg ccgccgtgcg gggaaccgct tcgaagccgg cgctgaacgg ccggcttcaa
     1801 ctccagaagg cttcgttcaa catggtcgat gtgccaaacg gcatttccaa tgccaatggc
     1861 gtggttctct tcaatagcaa cgaggccatc attcagaccc ttacgggcga gaccggcggc
     1921 ggaaaggtgt cgtttggcgg gacggtccgc tacggcggac ctgaaatggc gttcaatctc
     1981 caggcccgag cccagcagat tcgggttctg tatccgtcca atgtcagtac acagttgagc
     2041 gctaacctta ctttgaacgg cacgaccacg gacaacctgc tttcgggcag cgtcatagtt
     2101 caggatgtcg cgttctattc ccatacagac ctgggacgca tcctctccgg ggcggccgcg
     2161 ccgccccggg ctccagcagc gcagaccggc gttctcggcg gcatgcgctt cgatgtcaga
     2221 atcgatactt cctcggcgtt tcaattgcag accgcactga cgcaaaacgt gcaggcggaa
     2281 gctcatttgc aactacgagg ctcgccgtca tcgcccggaa tgctcggccg catcgacgcg
     2341 cagcaggggg aagtgatttt tttcggcagc aaatacagca tcactgccgg gacgatctcg
     2401 tttttcgatc ctaacaacat cgagcccgta gtgaactttg ctctcgagac gcaggcgcaa
     2461 gggatcaccg tggatattat ggtgcgcggg cctatggaca agctgaccat gacctaccgc
     2521 tccgacccgc ccatgcagtt ctccgacatc gtctcgctgc tcgccactgg gagagtgcct
     2581 tcgacggacc cggtgcttgc cgcgcagcag cctgcggcgc cccagcagag catgcagcaa
     2641 ttcggcgcgt cggcggtgct tagccaggct gtggctaacc cggtctcggg ccggctccag
     2701 agattgtttg gagtgacaaa actcaaaatt gatccacaaa tcgtcggccc cgaaaacact
     2761 cctcaggccc ggctgacttt ggagcaacaa atcaccaagg atctcgattt cacctatatc
     2821 accgatttga cccaaggcaa tcagcagatc gttcgcatcg aatgggccat cgacccaacc
     2881 tggtccgctg tggccgtgcg gcaagaagat ggacagttcg gcgtggatat ttactataaa
     2941 aagcggttcc ggtagaggcg atgagtccga gatactcgcc tcgctcgccg gcttttcccc
     3001 gggggaaacg aatccgatct cttacttttg gggcgccttc cggggggcag ggcgccgccg
     3061 ggactgcccg gaagcgcggg ttcgcgaaac agcttccaca agaatgcgaa ccggccttac
     3121 ggccgccagc gtgacttccg cttgcggctt ttccatgcgg tcgagcgcct gtttttgctg
     3181 gctcacccag cgggccggag cgggcctttt cgattccacg taccggagag cctggaggcc
     3241 gatcgttccc gcccgagaca gatccttcga gagcggtgcg gtttctttca aaagaaaatt
     3301 gttctggagc aggggctgga gccgggcgtc gttgtcgcgc caaatgagaa aagtctggcg
     3361 aacccaggcg cgctccgccg cgctattccc cggctcttcc acgattttcc gagctgcctg
     3421 ggagagggcg cggacgctct cgctctccgc gcgggccgcg tctacaaggc ggttgagcgg
     3481 cgtggaggtg gttttttcgc ggggatgccg cccgcgcaat ccctgcggct cgaccacgtc
     3541 ggacagcacc ttgagttggc cagtcgagcg cccgcccgta aggcggtcga gcatgggcca
     3601 gtaagtggag cggtgttgca cgcctgtcca gtcgaggccc cggctcacaa gggagagtct
     3661 tgcgtacatg gagtcgacgt cgcgaagctc ggccggagac cacagccttt ccgcgatcgc
     3721 ggccatgcgc ggccaaatgc gagaatcgac cgtttccgca ttcacgagct cggcccacat
     3781 gcacgcctcg ccgcccagga tgcgcgccgc ttcctcggca ttcagctcct gagcgccgtc
     3841 ccccagggga tcgatgccgt aatgatacga cgccgggctt aaatggtcta agtaatatcc
     3901 aaacgagagc aggccgcgat agccccgtcg cgcgccctgc gccagcgatt cctggcccct
     3961 ccacgactgc accacgatgt ccttcggcag gtcggggtgc aggatttcat cccagccgat
     4021 catgattttg ccatgcttct gcacgatcgc ctggatgcgc ttgttgaaat aagcctgaag
     4081 gtcgtgagcg ccggcgagat tgtgcgcttt gcaaaaagcc tggatggctg tgttttggtt
     4141 ccactgattg gctctcactt catcgccgcc aatgtggaaa tactcgtcgg ggaacaggcc
     4201 ggccatttca ccaatgaagc tgtcgagaaa gttatacgtt tcctcgcgcg tcgggtccat
     4261 caccgcgtca aagatgcccc agtggcgtcc gatttcatag ggtccgggcg cgctcgccag
     4321 ttccggataa ccgaccagcc agctcagcga gtggccgggc atgtcgaact cgggaacgac
     4381 gcgaattccc cggtcgcggg cgaaagccac gacgcggcgg atttgatcct gcgtgtagaa
     4441 gaggccgtcg gaaccctcct gctggagctt ggggtagatt ttgctttcga cccgaaaccc
     4501 ctgatcgtcg gagagatgcc agtgcagcac gttgagcttc accgccgcca ttgcctcgag
     4561 gtttcgcaga atcaccggaa gcggcatcca gtgccgcgag gagtcaatca tcaacccgcg
     4621 ccagggaaac cgcggcttgt cttcgatccg aacggccggc accgtgaacg cgtccggccc
     4681 cggcgcgatg agttgtacga aggtcgccat cccgcgcagt gcgcccgtaa ctgtgggcgc
     4741 ggagagacgc gctccagaag cggcaatctc caactgataa gattcgtctt cgtagagctt
     4801 agggggggct gatcccgccg acttgcatgc gatcaccagg gcggcgggcg agccgccttt
     4861 tccgatctgc cgtggtatgg gcacgccggt ttgccgtgag atacgcgcca gcaaacgcat
     4921 cgcggcggct tccagacgct ggtcagaata gccggagata gcgatcgaaa accgcgaatc
     4981 gattgtcagc gagccgctcc ccatagacag ccttgcgggc atcggcatga ggggcagggc
     5041 tgccgtttcg ccaggagcgg cggccaagat gaacccgcaa aaagcggctg ccaggaatag
     5101 aattcgagac aactggatca cctccgggag aatcttgggt ggagagccgc cgcttgcacc
     5161 gcgtccatcc gtgaggtccc cgttggctgg gctcgcagag gacaaacgga tattgcttcc
     5221 gaaggactct agactctcca cgttccgcca tgctacagcg gctcgcttcg gattgtccag
     5281 ttgccaagtg gggcggccaa cctggtctgc cgaagggcgg ctggttcccg aattagctag
     5341 ccgggtcgaa gcgcgcttcc accggcgagg attgcggcac ggttcgattc gatcaaggcc
     5401 ttcgtgcggg tgggcgtccg gtagtgcgac gatccggcgc ccgccataaa cggcggcggc
     5461 aattctcgtt ttatccacag ctttccaaca cagccgtgac ggcaagcttt tcagcatgcg
     5521 gcaaataaat ctgtgcaaaa gaccgcctgt cgcagaatat tcctctggac aatcagtcat
     5581 aggacggtaa tatacgtctt cccggcgcag aacgctccgt cacagcagac ctgaaaatct
     5641 atctcctgcc gggcagtgat ccggatttga tcgatccaca aggcggcggt ggtgtgcgcg
     5701 ggcgctttgt tttatggata ctctggccag acacgccgaa agagttgagt cgttgctgga
     5761 ggtgctgggc tccggcgcgt gggtggcgga tctgctcacc gacgttctca cgcattccga
     5821 aatttatgga acgcaccggt ccgacgcgga gtggctgaaa gagagaatca ccggcgctta
     5881 tctttttccg cgcggcggcg cagtgtgtcc cggcgagaat gggccgggcc gccggccgga
     5941 attccagccg gtggaccgac agtgggtgca ttcgctgctg cggacattag agtcgaacac
     6001 ctgggtcgcc gggtttaccg cggacgtgtt ggaccgctgc gctgccgatg gagtcggcgc
     6061 ttgcacgccg gagtggatcc aaatgcggct cacgcaggcg acagactttg cggcccgggc
     6121 gggaaccttc aaccaaacac cgggaacgcg ggggacgacc gtgctgaaca ttcatcaatt
     6181 cgctgagccg ggcaatcagg cggcttatgc ttttcgcgag atgccaagcg tcatcgccgt
     6241 ggccaacctg aagggtgggt gcggcaaatc cacgatcgcc gtgaatctgg cttgcgagct
     6301 ggcgagcaaa ggcaatgccg tggtcttggt ggacaccgat tcgcagggca cggcaagctg
     6361 ctggatcgcg agcggccagc ttccgatcca gggcgagtcc atgcctttgg agacggcgga
     6421 agacgccgag ctgctgacga acaagatcct ggcttccaat gcgcagtatg tggtcgtgga
     6481 cgcgccggcg cacgtggttg ccgccacgcg ggcggcgggc ggcctcgccg atttggtgct
     6541 ggtgcccgtg accgccagcg gagtggatct gcaagccacc gccggcgcga tggatttgat
     6601 acacaaggtg cgggcttcgc ggggaaaccg cgggccaaaa tgcttgatcg tgccctccaa
     6661 ggttgatcgc cgaacgggag cggggcgcga gatcgaggcg gcgttgcagc gatttggaga
     6721 ggcgatcggc cccgccgtgc atcagcgcgc agcgttcgcg gacgccttta gcgccggcaa
     6781 atgggtgggc cagtacgccc ccgacagtcc cgcccactat gatatcgcct cactgactgt
     6841 ggcggtgaaa aggacgcttc gaacgtgaac aagaattcgc agaatgggca cgcttcggcc
     6901 agcggcaagg tgaatggcct gccgccggtc tggatgtcgc tgccgtgccc caacctcatg
     6961 aaggttatca tcgcagcggc agacgagaag atggcgccgg tggcgattga aggctggttt
     7021 gagccggagt tccagccggc ggtccctccg gttcgcgagg ttcgcgcgcg aggcggttct
     7081 gaggtgattc catcgccccc tcccgcggcc gccggcgacg ctccaggccc gccagttcct
     7141 ctccccgatg tgttccgaga ccttgtacag gcgcggtccc gttctccctg ggccgcgcgg
     7201 cgctggatca ttccagccgt cgtcgtgctc gccattctct tcttgcttct gggcgcaaag
     7261 atggtgcgaa gccggcggct gaaccctgcc aacgccggga tagaaagagc tctttcaggc
     7321 gaatatggat caagcaaaca agatcgagtt ggccaacaag cattgccgtg agctttccca
     7381 ggaagtgcag gaagggaagg cgaaaatctc cgaatctctg gcgtgcgccc aagtgcttca
     7441 aatcgaggga aaaaccgacg aggccctgca agagctgtac cgggcgagag accgcagcga
     7501 gcatttcgtc gaacgcctgc gagccgcggc tcagatgcag atcgagctga aacagtacga
     7561 ggctgccgcg ggcgcctacc gcgagattct gcagctcgac gataaaatcc ggacggcgtg
     7621 cttcggcttg gcattatgcc tggaaaagct tgggcgctgg gaacaggcga acaagaaagt
     7681 gcagcgggcg ctcgatgtag atccccggcg ggcggaggcg caaatcgggc ttgcgacttg
     7741 cctgctgcat ctcggccaaa ccggagaagc gctgaagatc ttcgatgcgt gcctccagcg
     7801 ggagccggac tgcgaagggg cgctcctggg caaggcggtc gcgttgcagc ttgaccagag
     7861 gctggaggaa gccgcggaac tctaccagcg cgttctttcc ttgaacccca actctcaaga
     7921 cgcgctggcc aacatgatcg caatcggcat cgcgaaaagg gcgtatcaaa tcgtgcgcga
     7981 ttgttccagc cgattgctgg cgatccaacc acgatcacgg acggcgctcg aaggcctcgc
     8041 gatttccgct ttctccactc gggattacgc cgccgccgcc aagtattgcg gcgatctggt
     8101 ccaaatcgag ccgcacagct ttgcgaattg gttcaatctg ggggttgcgc agcagaaact
     8161 gggagatgcg ccggccgcgg cgagggcgta ctgcgaagcg cttcgcctcg attcccgcaa
     8221 gccccaagcc tatctcaatc ttggctgcgc gcagcaagaa atgggccaga ttgaggcagc
     8281 ccggagctcc tatgaaaacg ctttgcggct cgcgcctgac ctcgaaccgg cgctctggaa
     8341 tttggggctt ctgtcggagg tgattcagga ttccgagcga gccgagactg tgtactcgcg
     8401 attggtagag agaaatccgg gacacgagga ggcttggttt cgcctggctt gcgtccggct
     8461 ggcgcgcggc gatttcgggc gcagcataga ggcgttccag gaatgcctga aaaagcgcag
     8521 caagtggcac gaggcgttgt tgaatatggg catcgcctat tggcgcatgg gcgaccctga
     8581 ttcggcgcaa gagtacttgc acgaggcgct cgcctgccgg ccgaaatcgc ggcataccct
     8641 gggcgttctg gcggcgcttg ccattgaacg cgaagatttt gacgaggctc ttgagttgca
     8701 tggccgcctg gtcgatctcg gcgaacgcaa tcccgaggtg ctgtacaacg ccggatttct
     8761 tttccagcgc gcgggaaagc tggaagacgc ggcccgcgcc tatgaaaagg ccctcgcgga
     8821 aagacccaaa ttccgggagg ccctgttgaa tctggggcac tcctaccgct ccttgggcag
     8881 ggatgaggag gcgcaagcca tgtggctgaa agcagtggaa gccgcctccc ctgcggcggg
     8941 gagcggccaa ccggcgctcg atcgcccgca gtaaattcca gcaatgaaac tggcgaaaca
     9001 actcggccgc taccagatcc aggcggagat cggccgcagc gcgatcgcca gcgtgttccg
     9061 cgcgctcgac ccgccgctgg accgcctggt tgcgctccgc attcctgtgg acggcgctga
     9121 gggtttgttt cgcggtctgg cggaccaggc ggctgcggcg gccaagtggc atcaccctgg
     9181 cattgtcacc gtttacgaac tgggggaaca cggtggagtt cccttcatcg tcctggaata
     9241 cctttcgggt aagacgctgc gccagctcat cgccgaccga tccccactta ccgtgcttga
     9301 aaaaacgctg ctgatgtcgc aagtggccga cgctcttcga ttcgcgcacc agagcggcgc
     9361 cattcgcctg gatatcaggc ccagcaagat tatgctcatg ccggatggtt cggcccgggt
     9421 cttggacttc cttccgcacg gctggcggga tttctgccgc tccccggagg accgggcctt
     9481 gtatgctgcg ccggagtgcg gcgcttctcc gggagatatc cggagcgaca tttttgtttt
     9541 tggcgtggtc tgctatgagt tgctcaccgg ccttcaccct tttcccgacg aagaggcagc
     9601 gcgggccggt gttttgatgc cagcgagcag tctctgtccg gattgcccgg aatccctcga
     9661 acaaatcgtc cagcgcaccc tgcgccagga cccggaactg cgctaccaaa cgccgtggga
     9721 tctcaaactg gagctggatg gagttctgat tgaactgaaa agagagcgcg ccgccagctt
     9781 ggtcgccgaa agcgagagac tggctgctca gaacgagttt gagacggcgc aggccctact
     9841 caacgaagcg cttgcgttgg acccaggcaa ccgaaacgcc cggctcctgc gggagaccgc
     9901 ccggaagcag gcgcagcagc atttgctgga gcaggcgaaa gaggaaatcg ccgagcgccg
     9961 ctttgctagc gcgattcaga tgctcgaatc agcgttgcga atcgacgctt cacagggcgc
    10021 tattcgcgaa cgcatcgccg aagcgcgggc tctgcttgaa cggagcgaag cggcggacaa
    10081 gctgatcgaa caggcattgc ggaaattcga gcaggacaag ctcccggaag cccggaaaat
    10141 cgcggtcgaa gccgtcgaga cggaccctct aagcccccag gcggcggaat tattgcagct
    10201 tatcgaaacg gaaatcgccg ggcgcgaggc ccgcctcgag caatcgctcg ccgatgcgga
    10261 ggaactcctg agagcggagc agttcgatga ggctatcgcg ctgcttcggt cgctcgacgc
    10321 cgaataccca aactcgggga aaatcaaacg ccgcttggcg caggctcgaa gtaaaaaagc
    10381 ggaccggaag cggcggcaac ggctccaggc caagctggcg ccggtcgaat cgctgatggc
    10441 ggagcggcgc ttcgccgagg ctgtcgagcg cttacaggct ctgctgaaag aattcgggaa
    10501 agaaaagcag ctctttgaat ggcttttgca ggctcaaaaa gagctggaac gcgagcagcg
    10561 tagactggcg ctcgaaaacg cccggagcca ggtcacagcg ttttgccaag cgggagattt
    10621 tggagccgct ggcgcccacg ttttgggaac cctacagtcg tatcccaacg aacccgaact
    10681 gatgcagttg ttggaggaag taactgcctc ccagaaggct tgggaagagg gcgtggcgcg
    10741 gggcgtggaa gaagcggaat ggctgatgaa ccaaggccgg cccgatctcg cgctgccgct
    10801 gctggagcgg gatgtgggga cgtaccccgg ctccgggcgg ctgctcgatc tgctcgcggc
    10861 agccaaacag gcgctgccgg aatatgagcg gcgccagttc acccagcacg ccatcgcgcg
    10921 cagctccgat ctcgaggcgc gcggccagac gcacgcggcc ctcgcgctgc tgggcgaagc
    10981 gttaacccgg tatccgaaat ccgaggaatt ggccgcggcg gcccggcaga tgcgggcgcg
    11041 gcgcgatcaa gccgagcggc agcagagaat cgcgcagcgg ctggaagcga tcgaacgtaa
    11101 gattgccgaa caggcctggg cccaggctct cgtcctcgcc caggcggccc gatcggaatt
    11161 tcccggcgac tctgacttag agcgcgtctt acagagagcg gaagacggca aacgccgtgc
    11221 cgagcgagaa gacctgctgg ccggcatcga gcagagcgtg ctcgaagccg acattgaccg
    11281 agccctggcc aaaatcgacg acgcccttca gaagaacggg accgatcccg agctcgaaaa
    11341 ccgccgccaa gaattgcaac gcgagaagca ataccgcgaa agcttgcgcg aagcggaagt
    11401 cctgtttggc cgccggcagt tccaagaagc ggaaaacgcc gtccgtcagg cggctgccct
    11461 gaagccctgc gaaaacagcg cacgggtcct cctggaggcc attcgcgcgg agcgagccgc
    11521 cgaggaggag gccgagtttt atagccgcgg gcgggaaaaa gccgctcgcc tgattcgaga
    11581 gcgccaattc caccaggctt tggaccttct gcacaatttg ctttccctgt ttccagggga
    11641 tcccattctg gaaagagact tgcgcgcggc cgagagcgct cgaaacgaca gttctgctgc
    11701 cgccattgcc gaactgccgg tcgaggatcc ggatgtggag aaggcagacc tgctccccga
    11761 gtccccgccg gcacacgatc ccgcaccctc cggactatgt gccgttccgg aagctgctga
    11821 tccggatgcg ggtgtcggag atgttcatcc aagggcaacg gataatcctg cccttccgga
    11881 agttcctgtc ccatccgctg cggttgctcc gccgccggtg gccccttcgc cgcagcctca
    11941 gtttgccatc agctccggcc cttcctggaa gcggcccgcg tttgtggctc ttcccgctgt
    12001 gtttctcgcg gcggcggccg tgcttccgat ctggatttcc tcgcgcaagg cgccgcaggc
    12061 cgcttcggcc aggggaaccg tgacaacgcc cgccggttcg cagcccgccc cgcgcccggg
    12121 aatgcccgag cgcagcggca atttccggga tgatgggcgg gcttccaata tcccagagcg
    12181 accggtagaa accaacgaag gagtcgccca ctcgaaggct gcggaaaggc tgccggccga
    12241 acggcaagag cctgcgccga ggcttcctcg aaaatttgat atttccaggc tgcggcgcgg
    12301 agcttcggag caatccaaat tgacgcccga ggaacctttg tcgggcattt ccgtgccgga
    12361 cccagggaac ctccccgctg ccgtattcga gccgagcctg gcaaagccgg cggcgccccc
    12421 gcaagatgcg cccatggcaa cgtcaaaagc cgccgtcgaa cgaatccgta ctggcggcaa
    12481 ctttcagcag cccgccttta tcagcggacc cgcgcctgta atcccgccgg ctgcaaggga
    12541 acgcggcatt tacggcgttg tgaaaatgga actggcgatc aacaagcaag gcattgttac
    12601 cgaagcaaga ttgctaagcg gccctgcgat acttgtacct gcggctcgag aagcagccct
    12661 gcaacggcgt tatcgaccgg ccacactcga cggggaacct gtggaagtca aactgccaat
    12721 ccaatttgtg tttcaggcga gccggtaacc cggacgccac cgggggcaag cgccggcctg
    12781 agatctgccc ggtcctccaa aagccagcac tgcaagctga ccgtcgaacc tttctgtctg
    12841 ccggttcggc ggctgagggc gtgtcttcgc tgtgcgaaga acggtgcggt tttcccggaa
    12901 ggcggctggc ccagcgagcc atccgaaaga tgcgcttcgc gctcggcaat gggaaaatga
    12961 agacgtgttt cagttgcccg agaaggccgg ttggagaggc tcgcggcgtg ggtttttcgc
    13021 cgtactggcg gccggcctgg ggtctttggg ctggaatttc gccagccgca ggagctcatt
    13081 ccctttggct ggcgccggga gcgcttctat gtccgtctcg attgtcgagt tcaccgatgc
    13141 tggagaacgc aagggcgtgg tcactgtgga taagattgtc aaaaccgacg ccgagtggca
    13201 aaagcagctc gggacgggcc ggtttttcat cgcgcgcaag aaagatacgg agccggcctt
    13261 cagcggcgaa tactggaacc tgcatgaaga cggcatctac cgctgtgctt gctgcggcac
    13321 ggctttgttc gattcagcga cgaagtttga atcgggaacc gggtggccga gcttttgggc
    13381 gccgattgcc gaagagaaca ttcagactga aacggacacg agctttttca tggttcgcac
    13441 ggaagtactg tgccgccgct gcgacgctca cctggggcac gtcttcgagg acggaccccc
    13501 gcctaccggc cttcgctatt gcatcaactc ggcggcgctc cggtttgtga aagggccgga
    13561 cgcaagtagc tgattcctct tctggaaaat tcggctcaag cccgcggcgg gtggatttcg
    13621 attagagtgc ggtcgatgga ttcaagattc ggccgccccg aaagccccat cgccagagcc
    13681 agctcgctct ggagcagttc gagcacccgc tgcgcgcccg cctggccgta agcggccagt
    13741 ccccacagag gcgggcggcc gaccaagacg gctttcgccc cgacggcgag ggctttgata
    13801 atgtcagtgc cgcgtcggaa accgctatcc acaatgacgg gaacccggcc tccaacagcg
    13861 tcaatgatcg aggggagcac ctcgatggtc gaaggatcat aatcgagata gcggcccccg
    13921 tgattggaaa catggatgcc ggctgcgccg tgctcgatcg cggccttggc gtcttccggc
    13981 gtcaggatac cctttactaa tactgggacc ttggcgtagg aattcacttc ctggacaaaa
    14041 tcccaagtaa gctccgccgt aaagcgcttc tggagccgat aaggacgcgg cgcggccgcc
    14101 cggcggcgga cgctggattg ttctccccga tcgaccagtt gtggaagcgc gtctccgggc
    14161 gcaacctcct gctcctggcg gttgcgcagg aggcgctccc gatgagaaac gtagggcgtg
    14221 tccagggtaa tatcgataac tttgcagcct gcggcgagag cgcgctggac gcgctcccgc
    14281 gtgccagggt ggtcgggacc agggtagagc tgaaaccaca ggggaccagt cgccgccgcc
    14341 gcaattttct cgatgggaaa actcgagttg gtgctgacaa ccataatggt cttggcagct
    14401 ccggccgctt tagccattgc gagctcggct tgagggtgcg caagggcctg cgtacccgtt
    14461 ggggcaacca ggatcggcgt ttcgattttt tggccgaaca gcgtgaggga aagatcgagt
    14521 ttgctcacgt cgcggagcat atgcgggcgc aaagagatcc agttgaaagc ttcccggttt
    14581 cgccgtagag tccactcatc gtcgacgccg ccggcgatga agtcgtaatt cgctttgggg
    14641 attttggctt tggccaccgg ctcgaactcg agcacgttca ccagatcgtc cagcaggggg
    14701 acgcggtcgg actcgccggg aagctgctga gcctcgagcg ggggccgcca caagggcgag
    14761 cctgccagcc aaaccgcaag attgcgaagg gcgttgcggc gagtcatagg cctccttaca
    14821 gcggtgatga tgtaggcagt ttactgcgga ggcgagatgg tgtccaccct ggtgaggaca
    14881 actgggagaa aaacaaaccg gggccagtat ttgtccctaa attacaaatg gcagaagcgg
    14941 cagcctcgcg gagcattctt ctcgacgtgg cattccaggc aaccgcgcat gctgatatca
    15001 gtctccacgc acaacgtatc gcgctcgcgc acgggaccgt ggcaggcttc acaggtctcg
    15061 ttgccggtct cgacgtgctc tttgtggctg aagtccaccc aggcaggaag gctgtagacg
    15121 cgtgcccagg gcaccggttt agcctgcttg tgaaactcgg ccagcttctg tatgaaaggg
    15181 ctgtcccttt tcacgcgggt gtggcagctc atgcaagtct gagttgcagg caagccggcc
    15241 tcccatcctt ggcccggcat ttcatggcag cgcttgcact ggaggcgcaa ggccatgtgc
    15301 tgcttgtggc tgaacgggag aggttggacc ggagcgggcg cttcgcactc ggagcgcgcc
    15361 atcgggaggc acgctgcaca ggcaatcagc gtcaggacaa tccaccgccg gcgcatgagg
    15421 ccatcatgtc gcacgccagc ccggatttca acaatttgaa aaagaaacgg cgtttccgct
    15481 ttctgagcgt tgtctccctt tttccgcacg ttctacggag agccgctatt atgaagatgg
    15541 atcccgagaa gggtgggcgc ttcgattccc cagactgccc gccgggaata cggcgagcgc
    15601 cggatcatag cctttgcaaa acgaactacg cgccaaccgg gccggcaagt tccgcgcggc
    15661 aatttttcgc ggcctttatc cgatcatcga tacggctgcc tgcgcccggg cccagattca
    15721 gccggagtcc ctggcgcgag ccatcgcccg cgccggcgtt tcgatggcgc agtttcgcca
    15781 taagggcgcc ttcacgcggg aaattttcgc ccaggcggaa aacgtgggcc aaatcctcca
    15841 ggcggccggt gtcgcttacg tgatcaacga ccgcgccgac gtcgctctgc tgctcggggc
    15901 cgatgggctg catctgggcc agggggactt gccgccggcg agagtgcgtc ccgtggtagg
    15961 atcttcgctg gcgatcgggt actcaacgca taaccgctac cagcttttgg ccggcgatcg
    16021 ggagccggtg gagtatctgg cgatcggtcc gattttcgcc acaggatcca aggaaaatgc
    16081 ggatcccctg gtcggagttg aagaactggc acggctgcgg cggctcaccg cgaagccttt
    16141 ggtggcgatt ggcggcataa cccgcgccaa cgcccgcgcc gtgctggatg ccggggcgga
    16201 cgccgtggcg gtgatttccg acctgctggc tgccgacttg gaggcgcgcc tgaacgaatg
    16261 gctgatactg acgcagcggt aactagcacc acccggagcc cggggttgat ccagggtctg
    16321 ggcctgatcg atgcaacgac tctggtgatg gggtcgatga tcggctcggg gatcttcata
    16381 gtatcggccg acatcagccg gcaggtcggt tctcctggcc tgctgattct ggtttggctt
    16441 ctcgcggcgt tgctcacctt gatcgccgcc cttagctacg gcgagttagc ggcggccatg
    16501 ccgcaggccg gcgggcagta cgtttacttg cgggaagcct tggggccgtt aagcggtttt
    16561 ctttatggat ggaccctatt tctagtgatc cagaccggca cgatagccgc tgtagcggtc
    16621 gcatttgcca agttcagcgg cgtatttttt ccctggatct ccgccgccaa cccggtcata
    16681 gccgtcgggc gtttcggaat cagcacgcag caactggtgg caatcggcgt attgattttt
    16741 ttgtcgctgg tgaactcgtt cggcatccgg acgggagcag ccgtgcagaa cgtcttcacg
    16801 ttcgccaaga ccgccgctct gctgggctta gtgctcctcg gcttcctgct tggttccaat
    16861 gccctggcgg ttgaacagaa tttcaccgct ttctggcggg gggccggttg gagtttcgag
    16921 gtcttccggg ctgtggctgt agcaatggtg ggagcccttt tctcggccga tgcctggaat
    16981 aacgtaacgt tcacggcggg cgaggtgaaa aatccgggcc gcaatttgcc tctttctttg
    17041 ggcctgggtg cgggaatcgt attggcgctc tacgttgcca ccaattttgt ttacctgcgc
    17101 tggctgcccc tgtcgccagg catccagcaa gcccccgagg accgcgtggc gaccgcggct
    17161 gtgcagctca tgattgggcg cggcggcgag cggctgatgg cattggccat catggtctcg
    17221 accttcggct gcatgaatgg attgattcta gccggagcgc gtgtatatta cgcgatggcc
    17281 cgcgatgggc tttttttccg gcgggtcgcc gaaatccatc cccgctatca cacgccggcc
    17341 gtgtctctgg ccgtacagtg cgcctgggct tgtctgctta cgctgagcgg cacctacagc
    17401 gacttgctgg actacgtaat tttcgcggtc ctgctgtttt acatactaac gatctggggt
    17461 ttgttcgtct tgcgtcaaac ccgtcccgaa atgccccggc cctaccgggc catcgggtat
    17521 ccctgggctc cagccctata tattctgctg gccgccctca ttgaggtgat gttgctcatt
    17581 tacaagcccg gctacacctg gcccgggctg ataatagtct tattgggtgt accggtgtat
    17641 catctgtggc gacgccgggc gtagggccgg gaaaagcctg ccggaaggaa cgagcgggcg
    17701 gggcgtgccg tggcgtattg aggagcataa atggcagact tgctcatcca aaaaccgctg
    17761 aacctattga tgcgggaggc gaatgaagag ggggcgcaca cgctcaagcg tgtgctgggc
    17821 ccctggcagt tgacggcctt gggcatcgga gccatcatcg gcgccggcat tttcgtgctt
    17881 accggagaag cggcttatca aaacgcaggg cctgccgttg tactttcatt tatcgccagc
    17941 gccatcgcct gcggctttgc gggtctgtgc tacgcggagt tcgcatcgct gattcccatc
    18001 gcgggaagcg cctacacgta cggctacgcc accctgggcg agcttttcgc ctggatcatc
    18061 ggctgggacc tgattctcga atacgctctg ggcgcctcga ccgtgtcagt cggctggagc
    18121 ggctacttca tctcgctcct caattatttc ggaatccagt tgcccgcgtc gatctctact
    18181 tccacgttca ttgtcaatgc gcaagggcgg ttcgagaaca ctggagcatt caacttcatc
    18241 gcctttggca tcctgctggt catctcagcg atattggcga ttggaatcaa agagtccgcc
    18301 accctcaacg cgatcatcgt ctttctcaag gtggcgatcg tgctcctgtt tatcgccgcc
    18361 ggagcgccct acgtgaaccc tcaaaactgg catcctttta ttccgccgaa cgatggagtg
    18421 ttcgggcatt atggctggag cggcattctg cggggcggcg cgctggtgtt tttcgcttac
    18481 atcggctttg acgctgtctc gacggcggcg caggaatgca aggaccctaa gcgggacttg
    18541 ccgatcggca ttctgggatc cctcgggatt tgcacgctgc tctacatagc ggtatccgcc
    18601 gtgctcacgg gcctggtcag ctacaagctg ctgggcgtgc cccatccgat cgccgtgggt
    18661 atcgaggcga cccccttccg ttggatggcc tgggtggtgg agctcggggc cgtgctgggg
    18721 ctcggctctg taatgctggt gatgctgtta ggccaatccc gagttttcta cactatgtcg
    18781 cgggacggct tgctgccgga agttttttca cgcatacatc cccggtttcg aactccctat
    18841 gtcatcacca tacttaccgg ggccgtggta gcgatcgtcg ccgggctgct gcctatccgc
    18901 gtgctggggg aaatggtgaa cattggcaca ctgcttgcct ttgcgattgt ttccgcgagc
    18961 gtaattgttt tacgcgtgaa gcgtcccgat ttgccgcgcc cattcaagac gccgggttac
    19021 ccgtttgtgc cggtcctggg tgttctgact tctctggcgc tgatcggttt tctgcccgag
    19081 gatacctggt ggcgtctcat catctggctc atcatcggac tggtcattta cggcttttac
    19141 ggcatccggc acagcaaggt gcgggcggcg gccggcggcc gggttccggt cccgggcgac
    19201 tgagcctggc gggcgatcga gcctgggcct ctggcccgtt aagatgtccc ttcagcttcc
    19261 agcaccgatt tcccgcttgg gccggatcga gaaaattttc ctggtcctgg tcgtcgcgga
    19321 tgcagtggtg tggatggcct ctcgcctctt gcagcagcgc ctgcctggcg cgtcgctgct
    19381 tcccttcgcc gtgttcctcg cggcaattgc gctgctgata aagctggcgc gggccggctc
    19441 gcgaaagctg ctctggcggg tgcgaaaccg gatgattttg gtttttctat tcatcggatt
    19501 cgtaccgctc ctgctggtga ccgcgctggc cggacttgcg acctacattt tggccgggca
    19561 agtggcggtc tacatcgcca gcacggagct tgaccggcgc gccgaacgcc tgcgggacac
    19621 cgctgccgcg ctggcgtgga gcctcgaggc tacggcgcct gcccgccggc cggagctggc
    19681 ccagcggtat ctcagccggg ctgcccaggc ctggccggag cttcaggctt ggatccgcga
    19741 ctccaccgga acgaccgttt ttccagcggg ctcttccgtc gagccgccac gcagcgaagc
    19801 gcgcggaatt gtccggcgcg gcgacgggtt ttacctggta gcccggcctt ccgggtcttc
    19861 acctgtggtg ggcggcgagc cggccgatag cgctaacgtg cccccgccgg aaccggaggc
    19921 ggcgtccgga aatgtaaagc gcaaagatcc gctggacgtt gtcctgcttc aacctttgga
    19981 tcctgtcaac ctggcggcgc tggctcccgg gttaggcagc atcgagttct tgccgtggcg
    20041 gcgagtgctg atgcggcgac ccaacggggc tgccgccgag gtgaacctgc tgcccgagag
    20101 aagggtccgg ccggccgcgt tcgagggcgg cgagcccttg ccggctcccg ccaatcgctt
    20161 tgactacgaa gtgctctggg gcacttctct gtttgaggtc ggccgatgga gcgatcgcga
    20221 ccggccgacg cttgtcaatc ttctggtccg cacgcggcct tcggccgtat atcgcgtgct
    20281 gttcggccgc cgcgtggagg tggcctcgac ggtcaccgtt ttttctataa tcatcggcac
    20341 gctttttctc atggttgagc tggcttcggt agcgaccggc gtttccatta cgcgcacgat
    20401 caccaacagc gttcatcagc tgtatgaagg aacccggaga gtgaaccggg gagatttctc
    20461 ccaccgcatc cccgccgatg gccgcgacca gttgggcgag ctttcccgtt ccttcaacaa
    20521 catgacggaa tcgatcgagc ggctgatcga ggaatcgaaa gagcgccagc gcctggcctc
    20581 tgagttggaa atcgcgcgcg aagtgcagca gcaattgttc ccgaaagcgc cgccctccat
    20641 gcggcagctt gagatcctgg gtgtgtgcaa cgcggcccgc accgtctcgg gtgactattt
    20701 tgatttcatc aagatttccg acgatcatct tgcactggcc ttgggtgatg tggccggcaa
    20761 gggaatttcg ggcgcgcttc tgatggcctc gatgcaatcg atgctgcgta cccagttggc
    20821 cgtagcggcc tattttggca cgattttccg ccgcgatccc gccatgttca ccgctctttc
    20881 cggcgaactg tccggctccg ccgccaatga tgcaggctgg cggtttccca cggataaagt
    20941 ggtctcgcaa ctcaatcgcc agctttacga aaacacccct cccgaaaaat atgccacgtt
    21001 ctttctcgga gcctacgacg actgccgcgg gctgctcgct tacacgaatg ccgggcattt
    21061 gcctcccgtg ctcatccgca aagggcagcc gagccggctg gatgtaaacg gcatggtgat
    21121 cgggctgttt ccctgggcgc aatatgagcg aagcgagatc gagctgcagc cgggcgattt
    21181 gttggtcgcc ttcaccgacg gcgtcaccga gcccgagaac gagtttgccg aagaattcgg
    21241 cgatgagcgt ctgatcgatc ttctggtccg caactcggac cggccgctgc gggagatcat
    21301 tcaggaggtg accgccgccg taggcgagtg gacaggccgt ccggagctgc aagatgatat
    21361 gaccatggtg gttgcgcgcc ggctatgaga cttagaccga tccgctcctt cgggagccct
    21421 gaaccggctg catgcaggag accgtggaag cgagcgcaaa actgcggcaa atggaaaacc
    21481 gggtggcgat gaaatgaagg ttctcaccgc agcccagatg cgcgaaatcg accggcgcac
    21541 gagcgccgaa cgcggtgtcc cttccctggt gttgatggaa aacgccggcg ctcgcgtttt
    21601 tgagtttctc gcgcaccgct tttcgccgct cagccgcgaa cgcatttgcg tgctctgcgg
    21661 taaagggaat aatggcggcg atggcctggt tgttgcccgc cagttgctca ccaggggacg
    21721 gcccggacgg ctgaaggtaa ttctggcagc ccatcccgac tcgctgctag gggacgctgc
    21781 cgccaattac cgcatgtttc tcggcgtcga cggccaagta cgcgccgtaa cgtcggaagc
    21841 cgagtgggaa tcggcgctgg gcgaaattct ggattccacg ctggtggtgg acgctctgct
    21901 cggcacgggc ctgcgcgggc cggccgaggg cctgatgttg cgtatcatcc aggatgtgaa
    21961 tcagcgcctg cgccacgcga gcgttgtcgc cgtggatata ccttccggct tggccagtga
    22021 caccggggaa gcgctgggcg agtcggtccg agccggctat accgtcactt ttacggctcc
    22081 gaaagtgggc cagatttttc cacccaattg cgaggcggtc gggcagctct acgtcgcccc
    22141 gatcggcagt gcgcctagtc tctacgaaaa tgattccgag attttccttt ccaccatcga
    22201 ctccgaatac gtcgcgccgc tatttgcccc gagggcgcgc ggcgcgcaca agggcgattt
    22261 tgggcatgtg ctcgtggtgg ccggctccac gcccaagccc ggtgctgcga ttctggcggg
    22321 gacggcggcg ctgcgggcgg gagcggggct ggtaacggtg gccacgacgg cgcgcgccgc
    22381 ggcctcgatc acggcgcaca ccccggagtt gatgaccgag ccgctgccgg aaaccgaaat
    22441 gggcacggtg tcgcttgccg ctttcgatta ccagcgcttc gaggacatcc ttgccagaaa
    22501 aaatgtcgta gccatgggcc cgggcctggg tacgaacgac gaaacccggg ccttcctgcg
    22561 caaagcagtg gatctttgcg cgccgggcaa caggcgccag ccggtccgcc tggtgctcga
    22621 tgccgatgct ctggttcccg ataacgtccg tcccggcgtt gtaatgacgc cgcaccccgg
    22681 cgaaatggcg cgtctcacag gcctggatgc atcgtacatt caaaagcggc gggtcgagac
    22741 ggcccgggag ttcgcgtgct cgcgcggcgt gtttctggtg ctcaagggct accggacgct
    22801 tttggcgacg ccggaggggc gtgtgctggt gaacctgacc ggaacccccg cgatggcgtc
    22861 gggcgggacg ggcgacgtgc tcaccgggct tgtcgccggt tttttgagcg aatttcccca
    22921 ggcgcccatg gaacaagcga tcgctgcggc ggtctatttg cacgggcgcg ccggccagtt
    22981 ggctgcccag gagcttggcg aacaggccgc gctggccacg gatgtgctcc gacacctgag
    23041 ccgcgcgatc cgggagctca ccgaaacctc atgcggccag ccaccggcga tcctgccgca
    23101 gcctctctac gattccccct agccggccgg cagctacatg cccgaaactt ttttcactca
    23161 ttccgaagaa gagacgattg ccgcgggccg cgagattgcg aaacgtttgc gtccgcccgc
    23221 actggtattg ctgatcggcg agttgggcgc cggcaaaact acgctcgcca agggcatcgt
    23281 cagcggtctc ggggcggcgc gggaagagga cgtctcgagc cctaccttca ccctgattca
    23341 cgaatacggc aaagatccca aggtttttca catcgatctc tatcgcctgg atcgcccgcc
    23401 ggaactcgag accctcggcc ttgacgactt gtgggatcag aacgccatcg tactcatcga
    23461 gtggggcgag aagttcgccg cgcaactgcc gcgtgagcat ctcgagatcc ggctggagta
    23521 cgcgggagat gatgagcggc ggatcgcggt ggcagagtag ccggcaggtg attggccttg
    23581 gcggagagcg gtctgctacg atgacaaact tatggcaacc gaactgcaac cctgggcgct
    23641 gattctcgga tcttccagcg gctttggaga ggccaccgcc atcgagctgg ctcgttccgg
    23701 catggacatc tttggagttc attttgaccg gcgcgccacg atgccgcacg tcgaggagat
    23761 tcaatccaag attcgggctc ttggccgcga gccggttttt tttaacgcca atgcagtgga
    23821 tgagcagaaa cggaaggaaa tcatcgaagc gatccggcag cggtgctgca accctgcgtc
    23881 tccgggaacc gtacgcgtgc tgctccattc cattgctttc ggagcgctgc tgccgttcgt
    23941 ttcgcccgat gcggcggaga agaccgcgac caaagcccag ctcgaaatga ctgccgatac
    24001 gatggctcac agcctggttt actgggtgca ggatttggtg cgagcaggac tgatgacccg
    24061 cggtgggcgc atctacgcca tgaccagcgc cggcgggacc cgcgccattc cgcagtacgg
    24121 ccctgtctcg ggcgccaagg ccctgctgga atcgcatatc cggcagcttg ccctcgagtt
    24181 ggctcccttc ggcatcaccg ccaatgcgat ccgggccggc gtgaccgata cgccttcgtc
    24241 ccggaagatt cccgggagcg acaagctatt tgagttcgcc gccatgcgca atccccatgg
    24301 ccggctgact accccggagg acgtggccaa atgcattgct gtgctggcgc atgagaatac
    24361 ctactggatg accggcaaca caatcggcgt cgatggcggc gaggatatcg tcgcctgaca
    24421 cagccggcat gccgccgggt gaggcgctgc cgcgcggaat ggcctgttcc agccggtacg
    24481 ctgcggcctg accctgcctc cgcgcggctg cggtcgcccc atattagaac actaacttca
    24541 gcgcaaactg gaattgccgc gggttcaggg ccaaattcga ctgaatgatc tggccgaaat
    24601 tgcccgcctg ggtcggcgtg gccccggggc cggttccatt ccacttggtg atggttatgt
    24661 tgggctggcc gaaattctcc gtgttcgaga tgttgtacac ttccgcccgg aactggagtt
    24721 tcatccgctc gcggaacgga aaatccttaa acagagagaa atctacgctc ctctgtgtag
    24781 gcccatataa ctgagtacgc gcttcgttgc ccggcgtgcc cttcggctgg atcgtaaacg
    24841 cgttgagatt gatccagttt gtgatgcttt ggttcgctgg cacgtaagac tgtcccggca
    24901 ccgtgttggg gcgatcggtc gtcacgctcg tgacgttggt gggggcgggg ttaaacgccg
    24961 catccaggac ggtgaaaggt gttccggact gccagaatgc cagcgtgttg gcctgccaac
    25021 ccccatagac gaacttgcgc cagccgttca cagatttccc gaaaggaagc tcgtagttgg
    25081 cgcggaacga gaaccgatgc ttgatgccga tgtcggagtt accccaatca tagcttcggt
    25141 ttctcggcaa aatgccgccg acggtggcgg cccccgtcgt accgccatcg gaaatgttag
    25201 tcaggttgcg ggcgaatgta tagttcgcat tcaccgttat tcccttcgag tagcgccgct
    25261 cgaaaacaag ctccatggcg ttgtattcgg cggcgcccgt ggcagtgtag tggttgatgg
    25321 tgctgacgtt cggcaacaag ctggagtagt aaaacgggtt gagcgcccct gggctgggat
    25381 ccggaagatt ttcgttaggc gcatactgga gatgccgcgc cagctcgccc acgtagccca
    25441 cgctgacgac gttctgccca aactgttttt ggaacgtcag gttgtactgc atgatgtagg
    25501 cctggggata atgcgtgtca tgagcgccca gcacaccggg aatcagattg ttgagaggcg
    25561 gtatgacccc cagcggcggc cccgcagcca gcgtgccgat accgggagga cacaccagcg
    25621 cgccgctggt cgcagccggc tggcaattga agttggtgga aacaaacggc gcattcaaca
    25681 ggttcagcgc gccggaggca tagtcttgcg gaaagcggct catgccaaaa ccgccgcgga
    25741 ttacaatcgc tctccgggtg gtggcggcga agccgaaccg gggctcgaag ttggtgtaaa
    25801 cgggctgcac cggccccggg ttgctgccca ctttcatcgt gagcgtcccc aaatcgaagt
    25861 tggcccgctg ccctgtagcg ctggtgatgg gactgaaata gtcccaccgc agacccagat
    25921 tgagcgtcag ccagcgattg acgcgccagt catcctgcgt gtaaacgctg ggctcccaga
    25981 accggaaata ctgtttgtaa aagttgacct gccgctgaat ttgtgtgggc ttgccgagca
    26041 gtagattttc cagatttgtg agcgggtccg aaaggaattg gaaataccct agcccgaacg
    26101 tgttttgata atagttgagc tgtcgccgga tcaccccacc cccgaacttg atgttgtggg
    26161 cgcccctggt ccaggtcagc gaaccctgat actggaatac gttgttgatg tcgtaaatcg
    26221 gaacgaaccg gtcgtcgccg agtgtgaagc tgctgcccgg agcctggaat cccgaaacct
    26281 ggatcgttgc caggccggaa atctgttggc tcacatcggc gttgggcatg ccgaacttct
    26341 ggctggcatt cgtcccttga ttcggggaca gcgtctgcaa gttgaggcga atgaaacccg
    26401 ctttggcctc catgaccagt gtagggctga agatgtgcac gtagtccatc atgtagccct
    26461 gagaatccgc gtacgacggt cccgggaaga tcccgctgcc ggcgctgatt ccattcacat
    26521 cggggaaaag ggcgggctgc gagttgaagg tgggattgta cgagaatcgg gcgaagaaac
    26581 tgtcggagtc cgtgaaatga tgatcgagac gcgcgtcgta ggtcgtactg ttgtacactt
    26641 tcttgacgtt ccccgagtaa ttggcttgga ttccggcggc gccgggaaca ttcggcgccg
    26701 gccacagctt ccaatagttc aaggccacag gatcgagctg ggacggcgga aggaccgggc
    26761 ctccctggtc gctgaagttt cccggattca actcctcaaa ccgggtcggc actgacagca
    26821 atccggtcgg ctgcccctgc acgattctta gtgcttccgc gtcggcgaag aaaaacgttt
    26881 tgtttctgcg gatggggccg ccgaaggacc cgccaaactg gttctgatgg aagtccgggc
    26941 gcggcgtgcc gccggcgttc gaaaagaaat cgtttgcgtt taacttgtcg tttcggaagt
    27001 attcgtaagc gccgccatga tactcattgg tccccgattt cgtgatcagg ttgattacgg
    27061 cgccgccggc ccgtccgagt tcggcgctat aggcgttggt ttcgaccttg acctccgaga
    27121 tcatgtcgat ggaaggccgc atcagaatgg taaactgctc gcgctcgttg ttatccatcc
    27181 catcgatcaa ctggttgtta aacacctcat tctggccatt ggcagtgacg ttggcggttt
    27241 gacggcgttc atccgggcga gtgccgctca agatggaatt ggacggcccg acgcccacgc
    27301 cgactgtgct ttgcaccaga gtgatgtagt tgcggccgtt caagggcagg tcttgcacgg
    27361 cctgttccgt gacgaccgtc cggagcgtgg cgctgtcggt ttggagagcg ggcgactgcg
    27421 ccgccacttc caccacctgc gtgacttccc cgacctgtaa ctggacatcc gcgcgagcgc
    27481 ggtctccggc ggtgagcgtg aggttgatag tggctttctt aaagctgggc gcttcgaccg
    27541 aaaccgtata ctggccgggc tgcaaaagat tgactacata atcgccggag ccgctggact
    27601 gcgtggtccg gatttcgtgg gtggccaagt tctcgacggt aaccttagcg gtgggcacga
    27661 ccgcgccggc ggcgtcggtt actgtcccga ggatatcagc cgtgtttacc tgggcataag
    27721 cgatgctgcc tgcccacagg caccaaacga tccagcgcag ggcgcctgcg ccggcgagac
    27781 gtttcctcat tattttatcc ccctcgcaaa aaagcctgct cccttggaag tactcccctg
    27841 gaaaaaaggt ccctgtgcga ggccattcta ggcttgaatt ccccgagaat caatcgaaaa
    27901 ttcggcgtcg gccgggtttt ttacacttgc agcagactcg tccgacccat ggctgaaatt
    27961 ttcattaacg aaacgaaaaa tcggatggcg tccgattggt ggtatagaca atatccggca
    28021 acaagaaccg ccaagccaag gaccggggca acaagccgca gacgtagctg gatggaaact
    28081 gcgctgtgcg gccgcaggcg gctcggcatg ggttggattc taggtcatgg ctgttgcagc
    28141 cgctctgcgc atcggagtcc tgtgactccg ccgaatgcaa tccgcggttc tggcaggagc
    28201 aatcgggatc gatcggctgg gaccgagacc agcccccacc agcacacggg gagctctaag
    28261 cgtgggcgtg ctccccgctc tcctcagtga tcgccggatc gatgcgcctg ggcgtaggcc
    28321 agttcggtct gccggctggg ccgcgaggtt tcctgggcgt cgagtacagc gatggttgcc
    28381 atggtgacta cgtcctgaac gtcgaatccc ttttgcaaaa cggcgaccgg ccggctgagt
    28441 cccatcagaa tggggccgat ggcctcggcg ccagcgaggc gagtgagaag cttgtaagcg
    28501 atgttcgcgg cttcaaggca agggaaaatc aggatgttgg cttccgaacg cagccgggag
    28561 aagctaaaaa acctctcaat cagatccgga ttgagagccg tatcagcttg catctcccca
    28621 tcgacgatca gctcgggggc ccgctcgtgc agaatttcaa ctgctcggcg gaccttttcc
    28681 gaaagcggat gccggaccga gccgaagttc gaaaacgaga gcaacgcggc ccgaggctcc
    28741 acgccgaaac gccgggcgcg ctcggcggcg agcagcgtga tttcagccag gtcttccgat
    28801 gacggctcga tgttcaccgt cgtatccgcc agcagataaa tcttgcggtt gatgatcagt
    28861 gcataaagcc cggaaacccg atttaccccc ggccgcaaat tgatgatttg cagcgcgggc
    28921 cggatcgttt ccaaatagtg gcgtgtgatg ccggccacca tgccgtcggc gtcgccgcgc
    28981 cgcaccatca tcgcgccgaa agcgatcggg tcgcggagca tggcctccgc ctcgtccatc
    29041 gttacgcctt tccggtgccg gagcgagtgc agttcgcggg cgtaatccgc cgccgcgggc
    29101 gagcgggacg ggtcgagggt ctcccaggag gagttctgca acccgagctt gcgcgccagc
    29161 gacaagatgc gctcgtgatc gccgagcaaa atcggatgcg cgatcttttc atcggccagg
    29221 atttgcgcgg cccgcaagat cttctccgtc tcgccttcgg ggaacacaat ccgctttggc
    29281 gcttgcttcg ccctttgaat catcacgcgg gcgacttcct gcgccttgcc cagcctgcgc
    29341 gccaaggact cccgatattc ctcgaggtcg attttttttt gcgccacgcc ggattccatc
    29401 gccgcccgcg ccacggctac gggcacggtt tcgagcacgc gcgaatcaaa tggtttcggg
    29461 agtatatagc cggggccgaa ctccagccgt tcgttttggt acacgcgccg cacggcgtcg
    29521 ggcacatcct ggcgtgcgat ctccgcgagc gcgtacgcag cggcgagttt catctcgtcg
    29581 ttgatggcga tagcttggac atcgagcgcg ccgcggaaaa taaacggaaa gccgagaacg
    29641 ttgtttacct gattggggta atccgagcgg cccgtcgcca tgattacgtc tttgcgtgcg
    29701 gcgatggcgt cattgtaact gatctccggg tcgggattgg ccatagcgaa tacgatcggc
    29761 cggtcggcca tcgaccggag catatcctgt gtgacgcagt tagctatcga aacccccaca
    29821 aagacgtctg cgcccttcat agcctgctcg agcgtgcgca gattggtatc gcgggcgaag
    29881 cgctctttat aagggttcat gcctttggtg cgcccttggt agatcacgcc ttctgaatcg
    29941 cacatgagaa tgttttccgg ccgcactccc aggcggacgt acaggtcggc gcaggagacg
    30001 ccagccgcgc ccgccccgtt aaaaacgact ctcacctgat cgatcttctt tcccgtcagc
    30061 gccagcgcgt tcaccagcgc ggccccgctg atgatggccg tgccgtgctg atcgtcgtgg
    30121 aagacgggga tcttcagcgt gcgccgcagc gtttcttcga tgtagaagca ctccggcgcc
    30181 ttgatgtcct cgagattgat gcctccaaaa ctgagctcga gaagctgcac cacgtggatc
    30241 acgtcgtccg gattctggct gttcacctcc agatcgaata catcgatgtc ggcgaatcgc
    30301 ttgaagagga ctgccttgcc ttccatgacg ggctttcccg ccagaggccc gatgttgccc
    30361 aatccgagca ccgcagttcc gtttgtgaca accgccacca ggttggagcg cgatgtgtag
    30421 cgccatccca gttgcgggtc gcgtgcgatt tccagacagg gctcggctac gcccggcgtg
    30481 tatgccaggc tgagatcgag ctgcgtaaga catggcttgg taggcgtgat ggaaattttg
    30541 ccgggcgacg gcgaggcgtg atacgcaagg gcgtcctgct tccgaaatga catggggtct
    30601 cctcctgcga attttcccgc aaacggaatg aggcccgcaa ctttgccccg ggcgcgggcc
    30661 gaccctccgc cagcgcccgc acgcaagcaa ttgcttttgt tgtgttatcc tgccggggtg
    30721 gagatctggg aagtttttca atcactgggc cggccggccc tcgatgagtt ggtccgccac
    30781 atttctttgg gcactttaaa ggcgtacaag ctctacgagc cgttcaaaat ccgggcgcac
    30841 ttgacgaagc tgaacacgga acacctgcgg aaatcgaccg gtcgcttctg ggaccggctg
    30901 agcagcggag atgagggcct ggcaaaggac ttggcgcagg cgattctggt ctcgaacatc
    30961 gggttcattg tcgaggtcct caatttttta aacattcctc atgacgggaa cggttttctg
    31021 cccaaagact tttcgggaga gaaatacctg accgaaggct ggcaacagcg cgtcgaaaac
    31081 gaattccgcg gccgctatcc ggactcactg ctgcgtctct acatcaacca ccttatgtgg
    31141 gaggtggata aacaagcaaa aatatacgcc agttagcgcc gcgcggctgc tcccgccggt
    31201 ttggctcggc acgcgagccc gcgcggtttc ggcggcttat caaacgaagt aaactccata
    31261 cctacgggca taaaacatgg cgaagaaacg aatcggcatc ctgaccggcg gcggcgacgt
    31321 gccgggatta aattcggtaa tcaaggcggt ggtatatcgc ggcggcgaaa tcggctttga
    31381 cgtactcggc atccgcaagg gctgggaggg cctcacccac gtgaatttgt ccagcggcat
    31441 cgatgaccga tatgtgctgc cattgaatcg ggccaataca cgcacgatcg accggacggg
    31501 cggtacgttc ctgcacagct cccgcacaaa tcccgcgaag atgaaagtag cgaagcttcc
    31561 gccgcacctg gcccagaaag cgggcgcttt ccgggaggta aagccgggcg tcgtggatgt
    31621 gacgccggca gtcctggaaa atattcaggc tttgggaatc gacgcgctgg tcgtgatcgg
    31681 cggcgacgac accttgagct acgcgccggt cctcgaacag cacggcgtgc cggtgatcgc
    31741 tgtgccaaaa acgatggaca acgacgtgca gaacacggag tactgtatcg gtttttcaac
    31801 cgctgtaacg cgcgcagtgg ccgcgatcag ccggcagcgg accaccgtcg gatcgcatga
    31861 gcgcatcggc atttttcgaa ttttcgggcg ggacgccggc tacacggctc tttacaccgc
    31921 ttacgtgact tcgattcgtt gctgcattcc cgaattccgc tttgatctcg ccaagctcat
    31981 tgatctgctg atgaccgaca agcgcaataa tccgagcaac tacgcgttag ttgtaatttc
    32041 cgaaggcgca acctggcagg gaggcgaagt gaaggaatac ggcgaagccg acgctttcgg
    32101 gcatcgcaag aaggtcaata tcggcgaggc gctcagcgac gaaatcaaga aggccacggg
    32161 ggaagaaacg atcgtcagcg acctgaccta cgatctgcgc agcggcgagc cggatttcgt
    32221 ggacaagctg gttgccatca ccttcgctaa catggccctc gattgcctgg cggaaggcaa
    32281 acacgggcgg atgatggcca tccagaacgg ctgctatgtg gatacaaata ttcccgactc
    32341 ctcgctgggg ccgcgcaaag tggatgtcag cacaatgtat aactgcgacc gttaccggcc
    32401 caactacacg aacaaaaacg gctggccgat tttcttaacc cgcgtgtgaa cgggcgagcg
    32461 ccgcctttgc gctgccggca gcctctgccc gcctccattt tgtaaacgcg ccggcctctt
    32521 actgcgtgcc ttcccgccgc ggcgctgggc cgcggatccc ggaagctgaa ctccgggatg
    32581 cccggcctgc cggatcaaag gcttcctgct ccgttgagcc aaacgccgcc ggaaaccgca
    32641 gaaacgagcg cctttgttcc atgcccctat tcctggccgg cgcttagccc ggcggcgcca
    32701 tcgcggcttt agctaacaaa tcgcctgttt cctgccgatg agacggggag atgggaccga
    32761 tagccgaagg cggctcgcgg cggatgtcaa ccgggatgtt cggtccggcc ccttctgtaa
    32821 gcgcagagca gattgaggct gcgctggcca atctccggca gctgcccgcg tcggaaccgg
    32881 tggtgcaccg cgccctggcg cagttagacg gctgtgattt tatcgtcgct gagctgaaag
    32941 aaacgttgct ctctgatgcg gcgctggcgg cgcgcatttt gcggctggcc aactcggcct
    33001 tctttggctt tcgcagtgaa gtgcgaacga tgtctcaagc gattgtgctg ctcgggcaaa
    33061 atcggctccg aactttgctc caccgcatct tggcggacaa gatctggact gcccttggtc
    33121 aggagcgaat tgccgccgcc ccgctgcgca caatgtcgct ggcgacggcg acggcggctt
    33181 gcacgctctc ccagttgctg ttgcgcgaaa acgccgagga gatgctgctg gccggcctcc
    33241 tacataatat cggcgagctc ttcctgctct ctcagtttca ggcggagtat ctccgcatga
    33301 ttgcactggc cggggagctg ggtctgcgca gggccgccgc ccaggtattc ggaccgccgc
    33361 ccgaaatctg cggcaagctg ttgttggaaa tttggggctt cccgcggctt tattccgcga
    33421 tcgtggagca tatggactgc ccgctggatg ccaggcgtcc cgcggagtac gcctcggcca
    33481 tcgtgctggt ccacgcagcc cggagaatgg cagagccttt cgtcgccgga agcgatgccg
    33541 cggaggcgat tcgggcagtc ccctctgaag tctgcgacga gctgggattg cagccagacc
    33601 tcctagccga ggtcttccag acccttcccc acagaatgtc gctcgaacag cttcaggccg
    33661 cgcgctgacg ctttggagcc caacgtttag ccttgcaacg agagatgatg caacacggcg
    33721 tctgtcacct gcctggtgga cgccgatccg ccgaggtcgg ctgggatgtt tctgccctct
    33781 gccaaaaccg ccgccacggc ccgatcgatc gcctgtgcgg cgtcctgtaa gccgagaaac
    33841 tcgagcatca tggcggcgct caagatcgtc gccaaaggat tgacaacgcc tttgccggcg
    33901 atgtcgggcg ccgagccgtg gaccggttca aacatcgaag ggcaatcccg tctcgggttg
    33961 atattcgcgc tggccgccag gcccatgctg ccggttatga tcgccgcaat gtcggtgaga
    34021 atgtcgccga acaaattgga tgccaccacg acgtcgaaac tctccgggcg gcggataaaa
    34081 ttcatggcgg cggcatcgac cagcagcgac tcggtcgaga tatcgggata atcacgggcg
    34141 acatcggtaa aagttcgatc ccaaagcacc atgccgaagc cctgcgcatt tgatttcgta
    34201 accgaacaaa cccgcttctt cttgttgcgg cgccgggcga ggtcgaaggc ataccggatc
    34261 acgcgttccg ttccgcgccg cgtgaacacg gcggtttgta tcgccacctc ttccggcatg
    34321 tggtgataca cgaagccgcc gacttgtgag tattcgccct cggtgttctc gcgaacgacc
    34381 acaaaatcga tcgagccggg cgccttgccg gccagcggcg atgagacgcc cggatagaga
    34441 taggcgggac gaacgcaggc aaactgatca aacgtccggc ggatcggcag cagaagcccg
    34501 ttgagggtcg tgtggtccgg gatgtccgga tgcccgatgg cgccgaggaa aatggcgtcc
    34561 gcgggacgga gcacatccag cgcggccaca ggcatcatac ggccatgccg gaagtaatag
    34621 tcagatcccc aatcgtgatg tgtgtactcg aaggccgcgt cataccggcc gccggcagcg
    34681 ttcagaacgc gaattccttc cggtataact tcttggccga cgccgtcgcc ggcgatcacg
    34741 gcaattttga aagtgggcat agtgacggat acgcctcaac ccggaagcgg aaaatgcaac
    34801 agcgtgatgg ctgcgcccgt gcacgccttt tggccgctct tttcgggcat tccgggcagc
    34861 taaaacagga aatatctctg ggccatcggc agcacggcgg ccggctcgca ggtgaccaac
    34921 tctccgtcaa cccggacctc gtaggtttcg ggatctacct cgatatgggg tgtcgcatga
    34981 ttgtgaacca tctccgcctt ggtgatgttc cggcagccgc ggacggcgac cgcttttttg
    35041 gaaaggccta ttgtcttcgg cacgccggcc tcgagcgcag ctttggatac gaatgtcagg
    35101 ccggtgttgg cggcggcttc gccaaaagcc ccgaacatcg gccgataaag caccggttgg
    35161 ggcgtgggaa tcgatgcgtt ggcgtcgccc atcgtgctcc aggcgatgaa gccgcctttg
    35221 acgatgattt ccggtttcac gccgaagaag gccggtttcc aaagcaccag atccgccagc
    35281 tttccgggct cgatggagcc gatgatatgc gcaacgccgt gggtgatggc cgggttgatt
    35341 g
//
LOCUS       DASREK010000287        10509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_632034, whole genome shotgun sequence.
ACCESSION   DASREK010000287 DASREK010000000
VERSION     DASREK010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632034"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFA54_15520"
     CDS             <1..288
                     /locus_tag="VFA54_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018241018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZS52277.1"
                     /translation="GHNAIVKLDTGPNVAVDILFAHPRNKPSASLHESLGLATVDTPG
                     GIALKVDERRETSMRGIYAAGDLANPLMPSVTLASSHGATAGISAQQSMLV"
     gene            complement(481..618)
                     /locus_tag="VFA54_15525"
     CDS             complement(481..618)
                     /locus_tag="VFA54_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52278.1"
                     /translation="MFVAGVSFDGLSMMRSPAAIAVTTGFSDRFTGLFHGEMIPTTPS
                     G"
     gene            621..1175
                     /locus_tag="VFA54_15530"
     CDS             621..1175
                     /locus_tag="VFA54_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZS52279.1"
                     /translation="MLASMFSELFPEGQVAMVSGDGSAFSSLPFDHLVFTGRTAVGRA
                     VMKAASENLVPVTLELGGKSPTIVAKGSVQSRTVSAIVWGKLLSGGQTCIAPDYALVH
                     ESEINTFIESYDRLVKAAYPDGPISKDYTSIVNDQQYSMLVDLIDDARPRCTDHRGRD
                     TGPATPPVVLTLSRRPSCSASPTR"
     gene            1275..1556
                     /locus_tag="VFA54_15535"
     CDS             1275..1556
                     /locus_tag="VFA54_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52280.1"
                     /translation="MRSTTSAATTRTDARCSTARRRERHHQRHDHAAQDDLPFGGVGE
                     SGIGKYHGIEGFRTLSHAKGIFEQGRWNFIELLRAPFGKRAAALLNFLR"
     gene            2276..3157
                     /locus_tag="VFA54_15540"
     CDS             2276..3157
                     /locus_tag="VFA54_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS52281.1"
                     /translation="MSLENFKIFKDIAQTRRISRGAAMNGISQSAASQLIQHLERDLG
                     VELFDRSKRPLALTEAGKIYYEACRDLLRRYEQAQAEIESLKAEASGSARIACIYSIG
                     LHEMARRTAEFQQLHPKARIHLEYLRPDKVYEAVLTDQTDLGMLSYPSQSRDLKIVPW
                     RQERMILVVYPSHRLAGRDLVEPGELAGERFVSFDEDLAIRKALDRFLRDCGVTVEPV
                     LQFDNIQMIKEAVAIGSGISILPEPTVAEEVKQKRLAAIPFRGASLARPVGIIYRRGK
                     KLSPTAQQFLDFLRSRA"
     gene            complement(3432..4175)
                     /locus_tag="VFA54_15545"
     CDS             complement(3432..4175)
                     /locus_tag="VFA54_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52282.1"
                     /translation="MLASVIFSLALLAQLVDHTAPAPHPAADLSPEKRGDVYMARKMY
                     REAVDAYQEALKAQPKSYVLYNKLGIAYHQQLQLAQAQRCYERAIKLDNKYSEAINNL
                     GTIYYAQKRYRRAISSYKKALSLNPDSASIHSNLGTAYFARKRYKEASIEYAEALRLD
                     PEIFEHRGTFGSLLQERSVQDRARFHYYMAKTYAAAGYTDRAIAYLRRALEEGYKDKD
                     KIPEEKEFAGLRDNPEFQQLMAADVPVVR"
     gene            complement(4199..6742)
                     /locus_tag="VFA54_15550"
     CDS             complement(4199..6742)
                     /locus_tag="VFA54_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZS52283.1"
                     /translation="MRIVVPKGRWWGFWFGPVGKVLLGLFLIGAVTGVSVFGYYWTKY
                     ARLIDRKLSQGPFNTPSKIFAAPEAVYVGEQSSPADIVSHLLASGYSESTSNPNGWFR
                     VRPDAVEIFPGPNSYFAAEPAVIKFSGKKVARIVSLRDNTDQALYDLEPALITNLFDK
                     SREKRRLVHFDEIPKVLVDAIISIEDKRFFEHSGFDPLGILRAAWVDMREGHLGQGAS
                     TLSQQLARSFFLTPDKRWSRKAAELLITLQLEQRLTKQQIFEFYCNQIYLGRRGSFTI
                     DGVGEAAQAYFGKDLRELSLPEAATIAGIIQRPEYFHPFRNPERARERRNVVLKAMLE
                     NGYINKAQYDGAVRSPLNVAAGSAESIDAPYFVDLVNEQLQSRFSEKNLVTQTYRVYT
                     TLDLNLQRAAVEAVRYGMPLVDEQIKRQRRFRGKTPPTPQCALIALDPHTGEIKALLG
                     GRNYGMSQLNRVMAKRQPGSAFKPFVYAAALSTALDPNSGAVITPITSVDDSPTTFWF
                     DGKPYEPSNFKHELHGMTTVRTALAKSMNIATVKVAEMIGYDKVVELAHKAGMNEDIQ
                     ATPAAAIGSYDVTPLEIAEAYTAFANGGVRVTPYMIRSVRDQNGRTVLENHPVETRVL
                     DPRIAYMMTNLLEEVMRTGTAAGVRSRGFLAPAAGKTGSSRDGWFAGYTSDLLCIVWV
                     GLDDSTDLEIEGAKSALPIWTEFMKKAITYRPYRNPAPFEPVEGIVTAEVDPATGELA
                     TPTCPDRRMDVFIAGTQPAAYCRAHGGRPGAVPLLTTVAGWDTEPPKASLPAPNAVAA
                     DSKPDPTAGAPQRPHVAVQSVPLPPPEKPDAEKSGKKKGFFHRILDVFK"
     gene            complement(6834..7166)
                     /locus_tag="VFA54_15555"
     CDS             complement(6834..7166)
                     /locus_tag="VFA54_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52284.1"
                     /translation="MKSSRRWLAAALLAVFLYVVSSLAPSFFADFEFTRYLEEIARSP
                     QIHTQPPGAVRDQIVERAKQLHLPVKEGDVQVEVSGGSARVSVRYLVQVNLPGYTVKL
                     HFAPSAGR"
     gene            complement(7243..8952)
                     /locus_tag="VFA54_15560"
     CDS             complement(7243..8952)
                     /locus_tag="VFA54_15560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZS52285.1"
                     /translation="MRWSQLFIPTLRENPAEAEVISHQLLLRAGYIRPLAAGLYSYLP
                     LAQRSLRKIAEIIREEMDAIGAQEFYLPELHPAEIWQESSRWEAMGDNMFRLKDRWGR
                     EMCLGMTEEEVMTSIARAEVRSYKQLPQIWYQIQTKFRDEPRPKSGLLRVREFTMKDS
                     YSFDLDEAGLDVSYQKHHRAYCRIFDRCGLQYLVVEAHSGAMGGSQSHEFMIPSDAGE
                     DWIAVCPNGDYAANVEKAQSAAVPPAAADPQINAAPEPFATPSRKTIAEVAEFVGLPE
                     SSQIKSLVMVAGSKPVLVLLRGDHQLSEAKLASALGSEVRPAHSEEIRQWFGASAGSL
                     GPVGVSNMEILADEALRGRKNMICGANKDDFHLRYVTPGRDFEARYLDLRAAAAGDGC
                     VRCGSALELKKCIEIGHIFKLGYKYSESMGARVLTADGQEKTVIMGSYGIGLERILTS
                     AVEQGHDKDGMCLPAALAPFDLVITPVNISDVSQRQAAENLYADCRAAGLDVLLDDRD
                     ERPGVKFKDADLIGIPWRVNIGRKLAQGMVEIVERRSRQSRDVPLPGAAGQIQRLRAE
                     AVK"
     gene            9208..10494
                     /gene="glyA"
                     /locus_tag="VFA54_15565"
     CDS             9208..10494
                     /gene="glyA"
                     /locus_tag="VFA54_15565"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZS52286.1"
                     /translation="MPTLPPMARPLEEVDPEVWEAIQNETIRQHTHLELIASENFVSQ
                     AVLDAAGSIFTNKYAEGYPGRRYYGGCEYADRVEELVRERAKKLFGAEYVNAQPHAGS
                     QANMAAYAAVLQPGDTILGMNLAHGGHLTHGHHLNFSGKTYKIVPYGVSRETETIDYD
                     ELQRLAVEHKPKMIIAGGSAYPRIIDFPRMRQIADSVGALLLVDMAHFAGLVAAGVHP
                     NPCEWADIVTTTTHKTLRGPRSGLILAREKFGKAIDASVFPGLQGGPLVHIIAAKAVC
                     LLEAMQPEFVLYQKQVAANAKELAAQLTQEGFHVISGGTDTHLMLVDVFSKGVRGKDA
                     ELALHRAHITTNKNAIPFDANPPLNPSGIRLGTPAVTTRGLREPEMRLLGSLIAEVLR
                     NPADEKVIEGVKRRVLEIVERYPLYAWKMQAAGVSG"
BASE COUNT         2050 a         3334 c         3135 g         1990 t
ORIGIN      
        1 ggccataacg ccatcgtcaa gctcgatacc ggccccaatg tcgcggtcga catcctgttc
       61 gcgcatccgc gcaacaagcc gtccgcaagc ctgcatgaat cactgggcct cgctacggtc
      121 gatacgcccg gcggtatcgc cctcaaggtc gacgagcgcc gcgaaaccag catgcgcggc
      181 atttacgccg cgggcgacct cgccaacccc ctcatgccct cggtcacttt ggcatcatcg
      241 cacggcgcga cggcgggtat ctccgcccag cagtcgatgc tggtttagat ctccgatgac
      301 cgacgagctt cccattgtgc tcctccatgg ctccgccacc ggctcctact cctgggcccc
      361 ggtccgcatg ggcgtcgtcc agagcagtga ttacctcgag cgcaatctcc gtcgtttcct
      421 gcgcccggcc cgccgccaca tcgccctgga catgcggctc ggcagcaatc gcatcaagta
      481 tcagccgctc ggcgtggtcg ggatcatctc gccgtggaac aagcccgtga acctgtcgct
      541 gaagccggtg gtcaccgcga tcgccgcggg agaccgcatc atgctcaaac catcgaagct
      601 cacaccggcg acgaacaacc gtgctcgcgt cgatgttcag cgagctgttc cccgaaggac
      661 aggtcgccat ggtcagcggc gacggctccg cattctcctc cctgccgttc gatcacctcg
      721 tcttcaccgg gagaaccgct gtgggacgag cggtgatgaa agcggcgagc gagaacctcg
      781 tccccgtcac ccttgaactc ggcggcaagt ccccgaccat cgtcgccaag ggcagcgtgc
      841 agagccgcac cgtgtccgcc atcgtctggg ggaagctcct cagcggcggg cagacctgca
      901 tcgccccgga ctacgccctg gtgcacgaat ccgagatcaa cactttcatc gagagctacg
      961 acaggctcgt gaaggccgcc taccccgacg gcccgatcag caaggactac acctcgatcg
     1021 tcaacgacca gcagtattcg atgctggtcg acctgatcga cgacgctcgc ccacggtgca
     1081 cggatcatcg aggtcgggac accggcccgg cgacgccacc cgtcgtcctc acactctcgc
     1141 gccgaccgtc gtgctcggcg tcaccgacga gatgaagatc gcgcacgagg agatcttcat
     1201 ccccattctg ccgattttcg gctacggcaa tatcgatgac gccatcacct acgtgaacgc
     1261 acggccccga ccacttgcgc tctactactt cggcggcgac gacgcggacc gacgcaaggt
     1321 gctcgaccgc acgacgtcgg gaacgtcacc atcaacggca cgatcatgcc gcgcaagacg
     1381 atctgccctt cggtggggtc ggcgagagcg gcatcggcaa gtaccacggc atcgagggtt
     1441 tccggaccct cagccacgcc aagggcatat tcgagcaggg ccgttggaat ttcatcgagc
     1501 tcctgcgcgc gccgttcggc aagcgtgccg ccgccttgct gaacttcctc agatgaagac
     1561 ctcactggcg cccgcgggcc ctgagcaccg gccagcggta ttgttctcaa gctctaagca
     1621 tggcatcggt agcccggggc tccgcgtcga acgcgagcga tgtcgagagc tgccggggct
     1681 cgatgtccgc ctgcaggtcg gcgatcttcc tcggcggtgg ggatggcatc ggcgcctcgc
     1741 gaggaagcgg cgcggcgtcg gctcctgact ggccatggtg agtagcctct gccgacgagg
     1801 gtagtacccc caagacggcc aaaactgcgg atgaagtcat ggcatctcct atcaaaatta
     1861 gagaccaaaa tccgaatagc agcacagtaa cgccgccggg agcggcaaag caattgtcaa
     1921 aagggtgtat ttgtggtcaa aaagggacgc ccgggagagg aacctggtat caagttgcag
     1981 aattcgcgta ttgcgattcc gatgggccgc ttgctgacgc gcgggtccac ttgccggcag
     2041 ctctccgcaa aggctcataa ctcgacttga aagagaggca ggcgaaaata cgtcagcatt
     2101 tgaaggcgtg gaggcgagaa aaaaatggtc gatttttctc gaacagccgg caaaaatgca
     2161 tcggattttc ccacagccgg attttccacg atcttggcgg ccaaggaacg cgcggccggc
     2221 agccgggcag gtccccgccc gaagcggctc ggccgcttta tggcactata gcctcgtgag
     2281 cctggagaac tttaagatct ttaaagacat tgcgcagacc cggcggatca gccgcggcgc
     2341 cgccatgaac ggcatcagcc agtcggccgc cagccagttg atccagcacc tggagcggga
     2401 tctcggcgtc gagttgttcg atcgcagtaa gcgcccgctc gcattgaccg aggccggcaa
     2461 gatctactat gaggcctgcc gcgatctgct gcgccggtac gagcaggcac aggcggaaat
     2521 cgagagcctg aaagcggaag ctagcggatc ggcccgtatc gcctgtattt actcgatcgg
     2581 cctgcacgaa atggcgcgcc ggaccgccga gtttcagcag ctacatccca aggcccgcat
     2641 tcacctggaa tatcttcgcc cggataaggt ctatgaggct gtcctgaccg accagaccga
     2701 cctcggcatg ctcagttatc cctctcaatc gcgggatttg aaaatcgtac cgtggcggca
     2761 ggagcggatg atcctggttg tgtatccctc gcaccggctg gccggccgcg acctggtgga
     2821 gcccggagaa ctcgccggcg agcgctttgt cagcttcgat gaggatctgg cgatccgcaa
     2881 agcgctggac cgctttctgc gcgattgcgg cgtaacggtc gagccggtgc tgcaattcga
     2941 caacatccaa atgatcaaag aagccgtcgc catcggatcc ggaatcagca ttctgccgga
     3001 acccacggtg gcggaagagg taaagcaaaa gcgtctggct gccattccgt tccgcggcgc
     3061 aagcctcgcc cggccggttg ggatcatcta caggcggggg aagaagctct cgcctacagc
     3121 gcagcagttc cttgactttc tacgcagccg ggcctgacgc agggcgccgg caaacgggtg
     3181 cgcctggggt ttccgccgct ccgcggtcca cagcggcgcc agatgccttg ccgacaaatc
     3241 ttcgacgccg cccgcagccg gccagatcag cgatgctttc atcgaagctg caccgggcat
     3301 ccaggagctg aaacgaaatc acgggaggat cgagcgcccg ctcgttgttg cgcgccgcgc
     3361 ccgcgctcca gagtgcgcag cgggggcctg ggctcgctgg gagcgcaggc ggtttgccgg
     3421 cgggcgtggg cctaccggac gacgggaacg tccgccgcca tcaattgttg aaattcagga
     3481 ttatcccgca gtccggcgaa ttccttctct tcaggaattt tgtctttatc tttgtagccc
     3541 tcttcgagag cgcggcgcaa atacgcaatc gcccgatccg tatacccggc agctgcgtag
     3601 gtcttagcca tataatagtg aaatcgggcg cggtcctgca cgcttctctc ctgcaacagg
     3661 ctgccaaatg tgccgcggtg ctcaaaaatt tcagggtcga gccggagggc ttccgcgtat
     3721 tcgatagacg cttccttata gcgcttgcgg gcgaaatagg ccgtgcctaa gttgctgtga
     3781 atggaagcgg agtccggatt gagggacaac gcttttttgt aagacgaaat cgcgcgccgg
     3841 tagcgcttct gcgcatagta gatcgtgcct aggttgttga tcgcctcgga gtacttgtta
     3901 tccagcttga tcgcccgctc gtagcagcgt tgggcctgcg ctagctgcaa ttgctgatgg
     3961 taggcaatcc ccagcttgtt gtagagcaca taagatttag gctgcgcttt gagcgcttcc
     4021 tggtaagcat ccacggcctc gcggtacatc tttctcgcca tgtatacgtc cccgcgtttc
     4081 tcggggctga gatcggcggc gggatgcgga gcgggggctg tgtgatcgac cagttgggct
     4141 aatagagcca ggctgaaaat tacggaggcc agcatattgg cgcctccttc ttcaaggttt
     4201 atttaaacac atccaggatg cgatgaaaaa atcctttttt cttgccggat ttctcggcgt
     4261 ccggcttctc cggcggcggg aggggaaccg attgaacggc gacatggggg cgctgcggcg
     4321 cgcctgcggt gggatccggc ttggagtcgg cggccacggc gttaggcgcc ggcagcgaag
     4381 ccttcggcgg ctccgtatcc cacccggcga ccgtggtcag caaaggcaca gcgccgggac
     4441 ggccgccatg ggcgcggcag taggcggctg gctgggtgcc ggcgatgaaa acatccatgc
     4501 ggcggtccgg gcatgtgggc gtcgccagtt cccccgtggc tggatccact tcggctgtga
     4561 ctatgccctc cacgggctcg aacggcgccg ggtttctgta tgggcgatag gtgatggcct
     4621 tcttcatgaa ttcggtccag atcggcagag cggactttgc cccttcaatc tccagatccg
     4681 tgctgtcatc cagaccaacc caaacgatgc aaagcaggtc cgatgtgtag ccggcgaacc
     4741 agccgtcgcg cgagctcccg gtctttcctg ccgccggggc caggaaaccc cgcgaacgca
     4801 cgccggcggc ggtgcctgtg cgcatcacct cttccagcag attggtcatc atgtaggcga
     4861 tgcgcggatc gaggacccgc gtctcgaccg ggtggttctc caaaacagtg cgcccattct
     4921 ggtcccggac cgaccggatc atgtacggcg tcacccgaac gccgccgttc gcaaaagcgg
     4981 tgtaggcctc ggcaatttcg agcggcgtca catcgtagga acctatggcc gccgccggcg
     5041 tggcttggat gtcttcattc ataccggctt tgtgggccag ctcgaccacc ttgtcatatc
     5101 cgatcatttc cgccactttc acagtggcga tgttcatcga tttcgcaagc gccgtccgca
     5161 cggtggtcat gccatgcagc tcgtgtttga aattgctcgg ctcgtaaggc ttcccgtcga
     5221 accagaacgt ggtgggagag tcgtctacgc tggtgatcgg cgtaatcacc gctccgctat
     5281 tggggtcgag agccgtactc agagccgccg catagacgaa cggcttgaaa gcggagcccg
     5341 gctgccgttt cgccatcacc cggttcaact ggctcatgcc gtagttgcgg ccgccgagca
     5401 gagccttgat ctcgccggtg tgcggatcga gcgcgatgag cgcgcactgg ggcgtgggcg
     5461 gcgtcttgcc gcggaaccgg cgctgtcttt tgatctgctc atccaccagc ggcatcccgt
     5521 agcgcacggc ttccacggcc gcgcgttgca gattcagatc gagcgtcgtg tacacgcggt
     5581 aggtctgggt aaccagattc ttttcggaaa agcgcgactg caattgctcg ttgaccagat
     5641 ccacaaaata cggcgcatcg atagactccg cgctgcctgc cgcgacattc agcggcgagc
     5701 ggaccgcgcc gtcgtattgt gccttgttga tgtagccgtt ttccaacatg gccttgagca
     5761 cgacgttgcg ccgctcccgc gcccgctcgg gattgcgaaa cgggtgaaaa tattcgggcc
     5821 gctggatgat gccggcgatg gtggccgcct ccggcaggct gagctcgcgc aggtctttgc
     5881 cgaagtaggc ctgagccgcc tcccccacgc cgtcaatggt gaaactgccg cgccggccga
     5941 gatagatctg attgcagtag aactcgaaaa tctgctgctt ggtgagccgc tgctccagtt
     6001 gcagagtaat caacagctcc gccgccttgc ggctccagcg cttgtccgga gtcaaaaaaa
     6061 agctgcgcgc caactgttgg ctgagggtgg acgccccctg gcccaggtgc ccttcgcgca
     6121 tgtcaaccca ggccgctcgc agaatgccca gtgggtcgaa tccggagtgc tcgaaaaagc
     6181 gcttgtcctc gatcgagatg atggcgtcca ccagcacctt ggggatctca tcgaaatgca
     6241 caagccgccg cttctcgcgg ctcttatcga acaagttcgt gatcaaagcc ggctcaagat
     6301 cgtagagcgc ctggtcggtg ttgtcgcgca gcgaaacgat ccgcgcgact ttcttgccgc
     6361 tgaacttgat caccgcaggc tcggcggcga aatatgagtt cggacctgga aagatctcca
     6421 ccgcgtcagg acggacgcga aaccatccgt tgggattgga agtggattcc gagtaacccg
     6481 acgccagcag atgggacaca atatccgccg gacttgactg ctcgccgacg tataccgctt
     6541 ccggcgcagc gaaaatcttg gacggggtat tgaacgggcc ctggctcagc ttccggtcaa
     6601 tcagacgggc gtatttggtc caatagtagc cgaaaacgga aacgccagtc acggcgccga
     6661 tcagaaacaa tcctagtaga acctttccaa ccggtccgaa ccagaacccc caccatcgcc
     6721 ctttgggcac cacgattctc atatcgaggt ttccgcaatc agctccagcg cctgcttccg
     6781 gcatctctta tcatagcgct cgccgcgctc gccgcggaat ccgcccgcgc acgctatcgt
     6841 cccgcgctgg gagcaaaatg cagttttacc gtgtagccag gcaaattgac ttgcaccagg
     6901 tagcgcacgg agactcgagc cgacccgccg gagacttcca cttgcacgtc gccttccttg
     6961 accggcagat gcaattgctt cgccctctca actatttggt cgcggacggc gcccggcggc
     7021 tgcgtgtgaa tttggggcga ccgggcaatt tcttcgaggt accgcgtgaa ctcgaaatcg
     7081 gcgaagaagg agggcgccag gctggagacc acgtacagaa acaccgccag cagagcggcc
     7141 gccagccagc gccgggacga tttcataaga tcccatccgg ggcgcctcgt taagccgcgg
     7201 cgactttcag agcaagcggc ccgcgccggc tgccgcgctc actcacttca cggcctctgc
     7261 cctcaggcgc tgaatctgcc cggcggcgcc gggcagtgga acatcccgtg actggcggct
     7321 gcggcgctcc acaatttcga ccatcccttg cgccagcttt cggccgatgt tcacgcgcca
     7381 tggaattccg atcaggtcgg cgtctttaaa tttcactccc ggacgctcgt ctcggtcgtc
     7441 gagcaagaca tcaagacccg cggcgcggca atccgcatat aagttttcgg cagcctgcct
     7501 ctgcgagacg tcgctgatgt ttacgggagt tatgaccaaa tcaaaaggcg caagggccgc
     7561 cggcagacac atgccgtcct tatcatggcc ctgctcgacc gccgaagtga gaatgcgctc
     7621 caaaccgatg ccgtaggatc ccatgatcac tgttttttcc tgtccgtcgg cggtcagaac
     7681 gcgcgctccc atggattcgg aatacttgta tccaagcttg aatatatgtc cgatttcaat
     7741 gcacttcttg agctcgagcg cagagccgca acgcacgcag ccatcccccg ccgccgccgc
     7801 gcgcagatcg agatagcggg cttcgaagtc gcgtcccggc gtcacatacc gcagatggaa
     7861 atcgtccttg ttggctccac agatcatatt cttacgaccg cgaagcgcct catcggccag
     7921 aatctccata ttagacacgc ccaccggacc gagcgagccg gccgacgccc cgaaccactg
     7981 ccggatctcc tccgaatgcg ccgggcgcac ttccgaccca agcgcggagg ccagcttcgc
     8041 ttcgctcaac tggtgatcgc cccgcaaaag caccagaacc ggcttcgagc cggccaccat
     8101 cactaaactt ttgatctgcg agctttccgg cagtcccaca aattcggcca cctcggcaat
     8161 ggtcttacgg ctgggcgtgg caaacggttc cggagccgca ttgatctgcg ggtcggccgc
     8221 cgcgggcggt acggctgccg attgcgcctt ttcgacgttc gccgcgtagt ctccgttggg
     8281 gcaaaccgct atccagtctt cgccggcatc ggaaggaatc atgaactcat gtgattggct
     8341 tccgcccatc gcgccggaat gcgcttccac gaccaggtat tgcagcccgc aacggtcgaa
     8401 gatgcggcag tacgcccgat ggtgtttttg gtagctcaca tccagcccgg cttcatcgag
     8461 gtcaaacgag taggagtcct tcattgtgaa ctcccgcacg cgcaggagtc ccgatttcgg
     8521 ccggggctcg tcgcgaaact tggtttggat ctggtaccag atttgcggca gttgcttgta
     8581 gctgcggacc tccgcccggg cgatcgaagt cattacctct tcttcggtca tgcccaggca
     8641 catctcgcgc ccccagcgat ccttcagccg gaacatattg tcgcccatgg cctcccatcg
     8701 gctgctttcc tgccagatct cggccgggtg cagctccggc aggtagaatt cctgagcgcc
     8761 gatcgcgtcc atttcttccc ggatgatctc cgcgatttta cgcagagaac gctgggcgag
     8821 cggcagatag ctgtagaggc ccgccgccag cggccggata tagccggccc gtagcagcaa
     8881 ttggtgactg ataacctccg cttcggcggg gttctcgcga agcgttggaa taaaaagctg
     8941 gctccagcgc atagaaagtg taacggctct cagtcatccg gcgcttttag catcgtatcc
     9001 tctcggcagt ggcggcgcgc aaactcggcg ccgccgcaac ctggccagcg cgaacagttc
     9061 tgttcctggg ccggggtgaa ccgcgaccac aaagggcgga tcacgaagga ctggcggctg
     9121 acaactgaaa gcttagtggc cattctagca tggtaaaatc aacttccagg ccgcaaaacc
     9181 acaccacgaa gcctgggagg agcgctcatg cccaccctgc cccccatggc ccggcccctg
     9241 gaagaagtcg atccagaggt ctgggaagcg atccagaatg aaaccatccg gcagcacacg
     9301 catctcgagt tgattgctag cgagaatttc gtctcccaag ccgtactcga cgccgctgga
     9361 tccatcttca ccaacaaata tgccgagggc tatccgggcc gccgctacta cggcggatgc
     9421 gaatatgcgg accgcgtcga ggagcttgtc cgcgaaaggg cgaaaaagtt attcggcgcc
     9481 gaatacgtca acgcccagcc ccacgccggc tcgcaggcga acatggcggc gtacgccgcg
     9541 gtgctccagc cgggcgacac gatcctcgga atgaatctcg cccacggggg ccatctgacc
     9601 cacggccatc acttgaactt ttccgggaag acatacaaaa tcgtccccta cggcgtaagc
     9661 cgcgaaaccg aaaccattga ctacgatgag ttgcagcggc tggccgtcga gcataagccg
     9721 aagatgatca tcgcgggggg aagcgcttat ccacggatca ttgacttccc tcgcatgcgg
     9781 caaatcgcgg attcggtggg agcgctgctg ctggtagaca tggcgcattt cgccggtctg
     9841 gtggcggccg gcgtccaccc gaatccctgc gagtgggccg acattgttac cactacgacc
     9901 cataagacgc tgcggggacc aaggtccggg ctgattctgg cccgcgaaaa gttcggaaaa
     9961 gccattgacg ccagcgtgtt tcccggcctg caaggcggcc ctctggtgca catcatagcc
    10021 gccaaggcgg tgtgcctgct ggaagccatg cagcctgagt tcgttctcta tcaaaagcag
    10081 gtggcggcga acgcgaaaga gctggcggcg cagctcaccc aggaagggtt ccatgtcatt
    10141 tcgggcggaa ccgatacgca tctgatgctg gtcgatgtgt tcagcaaggg cgtgcgcggc
    10201 aaagacgcgg aactcgccct gcaccgggct cacatcacca ccaataagaa cgcgattccg
    10261 ttcgacgcca atcctccgct caatccgagc ggcatccgcc tgggcacgcc ggcggtcacc
    10321 acgcggggcc tgcgcgagcc ggaaatgcgc ctcctcggct cgctgatcgc cgaagtgctg
    10381 cgcaatccgg ccgatgagaa ggtgatcgaa ggggtcaaac gccgggtgct tgaaatcgtc
    10441 gagcgctatc cgctctacgc ctggaaaatg caggccgccg gcgtcagcgg ctgatctctg
    10501 catcgcttt
//
LOCUS       DASREK010000288        12135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1619199, whole genome shotgun sequence.
ACCESSION   DASREK010000288 DASREK010000000
VERSION     DASREK010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619199"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /gene="miaB"
                     /locus_tag="VFA54_15570"
     CDS             complement(<1..974)
                     /gene="miaB"
                     /locus_tag="VFA54_15570"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZS52287.1"
                     /translation="MGAKTFYIETFGCQMNVHDSEKVIGTLMAEGYRQVDTPEEAHLV
                     LYNTCSIRDKAEQKVFSRLANFKRQAGQGKIFGVLGCVAQHQGESIFEQAPHVGLVCG
                     SASYSKLPQMLVQLETGNRRVTGLDLDTDETFETPFTKRDNPFRAYITIIEGCDKHCA
                     YCVVPFTRGPERSRTSQSVMAEARQLAEAGYTEIQLLGQNVNSYRDPSPAGWDFAELL
                     AHIGRIPGLRRVRFTTSHPRDFTPRIVAAIEDNPVLCDHVHLPVQSGSDRVLARMQRE
                     YTRQQYLERIDCMKRARRPISITTDIIVGFPGESEEDFEQTLDLLDEV"
     gene            complement(1063..2745)
                     /gene="recN"
                     /locus_tag="VFA54_15575"
     CDS             complement(1063..2745)
                     /gene="recN"
                     /locus_tag="VFA54_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZS52288.1"
                     /translation="MLHQLEIENYAVIDRVRVSFHAGLNLLTGETGSGKSILVDALSL
                     LFGDKASSELIRAGKERARVAGLFEFSGPPDGVELENGELLVEREILANGKSRAYLNG
                     RLVTLAALREIAPRLGDIHGQHEQQDLFTPEAQMEMLDRFCGAMELRERAAGLFERWK
                     NAGASLAAMRAKEQEKLRLLDLWKFQQREIREANLRAGEDAALEEEKRALANLSRIQQ
                     GAGGAYEALYGASNSAASQVKSAARALEDLARFDSLFGRLAQALESARISLEETAFEL
                     RQRLDRLEANPERLGEIEDRLALIERLKRKYGPSMEDILAFLGQVEKQVGELESSEEM
                     IQRLEKERCELAAQYEEAAGQLSRRRRQGAERLEKPVERELAALAMERTRFSVAFDAG
                     PPGVEGWGPNGFDRLCFLVSPNPGEPLRPLAQVASGGELSRLTLAIKTVLAASDRKPD
                     PLPRTLVFDEIDAGIGGRAAEAVGRRLQRLSRSFQLLCVTHLPQIAGFADHHYVVGKQ
                     IKAGRTITTVTEIEGGERVRELARMLSGAQVTTEAVRQAEQLLQAARAEVRG"
     gene            2806..3495
                     /locus_tag="VFA54_15580"
     CDS             2806..3495
                     /locus_tag="VFA54_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZS52289.1"
                     /translation="MTLPGSVAFLLLQTGIVQMILDAGLVAKVVIVVLLLFSLLSWTI
                     IFSKLGALRSAQDRNEKFLKAFRRTQKLAEMNTLAAQFHPAPLAVVFEFGYQEVERQV
                     NGKGRLHNIPALQRAMQLGSGEEVTRLERNMSWLATTASATPFIGLFGTVWGVMDAFQ
                     GLGQEGGASLRAVAPGISEALITTAFGLAAAIPAAIFYNYFLHRIKEVGSRMDDFGLE
                     FVNLAERSFGE"
     gene            3502..3921
                     /locus_tag="VFA54_15585"
     CDS             3502..3921
                     /locus_tag="VFA54_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZS52290.1"
                     /translation="MAFSPRNGRGSGALAEINVTPFVDVVLVLLIIFMLTAHVMEYGV
                     EVDVPRTKQVANTSKELPVVNISSKGELYLNSEPVNINDLVKKLEPMLGKDRAAYLRC
                     DKDVTWEIPVQVMAELGHAKVQVSVVTQPLEQLPRRK"
     gene            3918..4730
                     /locus_tag="VFA54_15590"
     CDS             3918..4730
                     /locus_tag="VFA54_15590"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZS52291.1"
                     /translation="MTRHLDVLEQRESLRLPFLESLGVHLLVLGYAAFYSWMVVHNRV
                     QFGNPDAMAGGAVPINIVNSIPMAPAQTVSENPVANDTRSSVPAPPPEKAKSREPVEQ
                     ADETAVPLPTAKTPKASKRQAPEKKFRPYVPSRDNQLYAMKGMGVNTPTYSGLQSENF
                     GVGVGVGAGSPFGAYFGWYAEALQRRIGEQWQKELMQLDQRISSVPRTVVFFEIQRDG
                     SLRNIRLVQSSGNSAVDYAALRAVNESNPVPPLPSGLAKNYVSTEVWFQIKR"
     gene            4727..6175
                     /locus_tag="VFA54_15595"
     CDS             4727..6175
                     /locus_tag="VFA54_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HZS52292.1"
                     /translation="MKDQRVAGALGTLVLAGLVIGIVSRAQQRPDITIPVVGERAPIR
                     IAVPDIPGSGVSADVNRVFNETLWNDLENSGVFQMVSKSLYPTEVPQKPEDFKPPLPA
                     PAGPQRRMRPGETPPPPPAPIRQGPWLTDWSQPPVQANVLAFGNTAVQNGQFTLSGWL
                     FDVTKNDTASAYVFGKRYFSSNDANGARLVAHDFSNDILQKLGAGQGIAGTRIYFVSN
                     RTGGGHKEIWAMDYDGNNQAPITRYNSICLSPDVSPDGSMIAFTTFVRGRPEIFIHSL
                     ETNRRLTFYNQAASLNATPSFSPDGKRLAYASSASGHAQIYVSNIDGSGFRRITFSQA
                     PEVEPKFNPKTGAQIVFVSGRSGQPQIYLMDSDGTNVQKLTPNMNGNGDAVNPAWSPD
                     GQRIAFSWTRGFEPGNYNIFIQEIATRNITQLTYGAGRNEHPSWAPDGRHIVFSSDRS
                     GSVQIWTMLANGTQLKQLTTQGRNEMPVWAAK"
     gene            6261..7037
                     /gene="pal"
                     /locus_tag="VFA54_15600"
     CDS             6261..7037
                     /gene="pal"
                     /locus_tag="VFA54_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZS52293.1"
                     /translation="MRRKRELIVILLGLCLALFAAGCKKKAPPPPPPPPPPAPAPVQP
                     APPPAPTVTFSAEPSSIERGQSATLRWSSTNATEASIDHGIGTVGTSGTRQVSPAEST
                     TYNIVVKGEGGEARASASITVRAPAPPPPPPPPRRSFSEDLASRVQDAYFDFDKYDIR
                     PDARQVLTSDADALKSLFNEFNTAHVVIEGHCDERGSAEYNLALGDRRANSAKEFLQE
                     LGVPADRLSVISYGKERPQCTEHNEECWQKNRRVHFTAGS"
     gene            7128..8009
                     /gene="ybgF"
                     /locus_tag="VFA54_15605"
     CDS             7128..8009
                     /gene="ybgF"
                     /locus_tag="VFA54_15605"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HZS52294.1"
                     /translation="MPRRLLFLFLLAALPAFPVAKEILQIQRDIALLQDQVRNLQSAF
                     DKQLAVTQQLLNQNLDANNQLSNRLAVLEKSLQDQGKVLVTPLANTNARVDALAGQVQ
                     GLRDTLDEMNSRLSRLQQQMVDIKNIVSSVPAPAAPATGVPGSTVAPGAPAVSAEKLF
                     SDAVRDYQGGNFAMAGPEFTAYIQQYPNTPQAAEAQYYLGDIFYQQKQYDQAAEAFDQ
                     VLERYPEGPRTPDAQYKKGMSLLNQGKRDAAAREFRGVISKFPDSTAASAAKEALRNL
                     GLSARSAPGKSTKSRKK"
     gene            complement(8053..9060)
                     /locus_tag="VFA54_15610"
     CDS             complement(8053..9060)
                     /locus_tag="VFA54_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52295.1"
                     /translation="MRALALFLLALLAPAAKDSGHISNIRQLTFGGSNAEAYWSPDGK
                     RLVFQSTRDGRECDQIYIMNADGSGVHMVSTGKGRTTCGYFLPDNKTFVYASTHLGGA
                     GCPPPPDRSKGYVWPVYPTYDLFVATDRGQILRQLTKEPGYDAEVTVNWKDRKLVYTS
                     MASGDLDLWSMNLDGSNKKQLTHEVGYDGGGVFSRDGKKLVWRGNHPKTPEALDAYKK
                     LLADNLTTPMKMELYLADADGKNVQQLTNYGCASFAPTFSPDGRKILFSSNKNHCDSR
                     QFELFLMDTDGNNVEQVTSFGGFTAFPEFSPDGKQIVFISDRNAKQRYEFNVFVADWR
                     W"
     gene            9201..>12135
                     /locus_tag="VFA54_15615"
     CDS             9201..>12135
                     /locus_tag="VFA54_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZS52296.1"
                     /translation="MLKLKKVELLGFKSFCERTEMHFNGDGIVGIVGPNGCGKSNISD
                     AVSWVLGEQSARLLRGTRMEDVIFNGTRDRKPLGMASVTLTLIDPEAFSPAEPEPVAP
                     ADAADAPPAGAPQNGSARARRRPGERPGEIIVTRRLFRSGESEYLLNGRLCRLRDIQD
                     IFMGTGLGPESYAIIEQGRIGQILSSRPHDRRAVIEEAAGITRFKTKKKLAEAKLESA
                     RQNLARVNDILEEVSRQVNSLKRQAGKARRYEELRAEMNAKLTVVLASRHRDMERLAV
                     QAALDLNLAAEEFRSCNARVERLEAEFGAAQKESFDLESQLQQSREACAAAAREAERT
                     QQRIDYQARLAEENRTRIGQAETEIAGIESRLEGLRKEIAAERSAADLVSAERSAVRE
                     QIETGTSELDGIQSHIREREREQEELRRRVICLLGQASSLRNQLAQIDEFLAGLERQS
                     ARARSEEAAANEEIAAVSAYRDSHLARLREQQLQLESLDESRRRLEASLAEAQRQAQA
                     RREQVQLLKEEISRIRARRESLEEILSHRAYTTETVKSLFAAMEQGRMEGFRPLGIFA
                     DFIEVDAEYEKAVEGFLREELEYVVVSNWEEAERGMRLLRSEMEGHATFLVYPGAQPE
                     AGAAGEAQSSPLASQPGVRGPLRAHVRFNNGLSHAASALLPRLDRCYLAENEETARHL
                     ACQHPDFYFLLPEGLCYHGCSLSGGRKTSSGPLALKRELREIMPRLAAAMQQLEAGSA
                     ALSQAEAQIASETAALEALRQEMQNREKGTLASEHELRQIHDRLHRANARLSAARLEI
                     DRFRQESERAAVEADRCRSQAADLENQQREAEEMLAAIRRAVAEDQARGAKAAEKLSE
                     WRAQLAALEERKKAAQAAAARLEQTFAGEARRRGEMAQQIEAWAAERERLLRENRDLQ
                     GQLESAAARQCALQQEGRNLEAALNQRRSRMAEIEESLKTARQQLEAARGRRSTIEIQ
                     LVRLQS"
BASE COUNT         2409 a         3780 c         3684 g         2262 t
ORIGIN      
        1 tgcacctcgt ccagcaggtc gagcgtctgc tcgaaatctt cttcgctctc acccgggaac
       61 ccgacgatga tgtcggtggt aatggaaatg ggccgccgcg cccgcttcat gcagtcgatc
      121 cgctccagat attgttgccg cgtatactcc cgttgcatgc gcgccagcac ccggtcggag
      181 cccgactgaa cgggcagatg gacgtgatcg cacagaaccg ggttgtcctc gatggccgcc
      241 acgatgcgcg gcgtaaaatc acgcgggtga gatgtcgtaa accgaacccg gcgcagcccg
      301 ggaatccggc cgatgtgcgc caacagctcg gcgaaatccc atcccgccgg cgagggatcc
      361 cggtagctgt tcacattctg cccgagcaac tggatctccg tgtatccggc ttccgccaac
      421 tggcgggcct cggccatcac gctctggctg gtgcggctgc gttcggggcc gcgcgtgaac
      481 gggaccacgc agtaggcgca gtgcttgtca cagccctcga tgatcgtgat gtaagcgcga
      541 aagggattgt cccgcttcgt gaacggcgtt tcgaacgtct catccgtatc caggtcgagg
      601 ccggtgaccc ggcgattgcc cgtctcgagc tgcacgagca tttgcggcag cttgctgtag
      661 ctcgctgaac cgcacaccag ccccacatga ggcgcctgct cgaatatgct ttcgccctga
      721 tgctgggcca cgcagccgag cacgccaaaa atttttcctt gccccgcttg ccgcttgaaa
      781 ttagccagac gggaaaacac cttctgctcg gctttgtcgc ggatgctgca cgtattgtag
      841 aggaccaggt gagcctcttc gggcgtatct acttgccggt agccttccgc catcagcgtg
      901 ccgattacct tctcggagtc gtgcacattc atctggcagc cgaaggtctc gatgtagaaa
      961 gtcttggctc ccactcaccc ccgattgtag cacgccgccg tcgaacctcc ggtccagccg
     1021 ttaagtgggg aagccccgga gaactttttc cgcgaaagaa cgtcagcccc gcacttcggc
     1081 gcgcgccgct tgcagcaatt gctcggcctg gcggacggct tctgtcgtca cctgggctcc
     1141 ggaaagcatg cgcgccagtt ctcgcacccg ctcaccgccc tcgatctccg tcaccgtggt
     1201 gatcgtgcgc cccgctttga tttgcttgcc gaccacgtag tgatggtcgg cgaaaccggc
     1261 gatttgcggc aagtgggtga cgcataaaag ctgaaaggag cgggaaagcc gttgcagcct
     1321 ccggcccacc gcttcggccg cccggccgcc gatgccggca tcgatctcat cgaaaaccag
     1381 cgttcgagga agcgggtccg gcttccggtc ggaggccgcc aggaccgtct tgatcgccag
     1441 cgtgaggcgc gagagttccc cgcctgaggc tacctgcgcc agcggccgca gcggctcgcc
     1501 cggattgggc gacaccagaa aacacaggcg gtcgaagcca ttcggccccc acccttccac
     1561 gcccggcgga ccggcgtcga aggccaccga aaaacgagtg cgttccattg ccagcgcagc
     1621 cagctcccgt tccaccggct tttccaagcg ctccgcgccc tggcggcgcc gtctcgagag
     1681 ctggccggcc gcttcttcgt actgcgcggc cagctcgcaa cgctccttct ccagccgctg
     1741 aatcatctct tcgcttgact cgagttcccc gacttgcttt tccacctggc caaggaacgc
     1801 cagaatgtct tccattgacg ggccgtactt ccgcttcagt ctctctatca gagcgagccg
     1861 gtcttcgatt tcgccgagac gttcgggatt cgcctccagc cggtccagac gctggcgcag
     1921 ctcaaacgcc gtctcctcga ggctgatgcg ggcgctttcc agcgcttggg cgagccggcc
     1981 aaagagagag tcgaagcgcg caagatcttc cagggctcgc gcggcgcttt tcacttgcga
     2041 cgccgcggag ttggaagccc cgtagagcgc ttcataagcg ccgccggccc cttgttggat
     2101 ccgggacagg tttgccagcg cccgtttctc ctcttcgagc gcggcatcct cgcctgctct
     2161 aaggttcgcc tcccggatct cccgctgttg aaacttccac aggtccagca gccggagctt
     2221 ctcctgctcc ttcgctctca tcgcggccag gcttgcaccg gcgtttttcc agcgctcaaa
     2281 caggcccgcc gctcgctcgc gaagctccat cgcgccgcaa aaccggtcga gcatctccat
     2341 ctgcgcctcc ggcgtgaaca ggtcctgctg ttcatgctgg ccgtgaatgt cccccagccg
     2401 tggggcgatt tcgcgcaggg ccgcgagcgt gacgaggcgg ccgttgagat aggctcgcga
     2461 cttcccattc gccagaattt cacgctccac cagcagctcg ccgttctcca gttccacgcc
     2521 gtccggaggg ccggaaaact cgaacaggcc ggccacccgc gcccgctctt tgccggctcg
     2581 gatcaattcg gacgaagctt tgtctccaaa cagcagcgaa agcgcgtcca ccaggatcga
     2641 ctttccgctg ccggtctctc cggtgagcaa attcagcccg gcatgaaaac tgacgcgaac
     2701 tctgtcgatg acggcgtagt tctcaatttc aagctggtgt aacatggaac cccgtccatt
     2761 atatgtgtta aatttcggtg ttggagctat actgaaggag atctgttgac gctacccggt
     2821 tctgtggctt ttctattgct gcaaaccggc atcgtgcaga tgattctcga tgccggcctg
     2881 gtcgccaaag ttgttattgt cgtgttgctt ctcttctccc tgctttcctg gaccattatt
     2941 ttttccaagc tgggagccct gcgcagtgcg caggatcgga atgagaagtt cctgaaggcg
     3001 ttccgccgca cccagaagtt ggccgagatg aacacgctcg cggcgcagtt ccatccggcg
     3061 ccgctggcgg ttgtgtttga gttcggctat caggaagtgg agcggcaggt gaatggaaaa
     3121 ggccggctgc acaatatccc cgctttgcaa cgcgccatgc agttgggatc gggtgaagag
     3181 gtcacccggc tcgagcgcaa catgagctgg ctcgccacca cggcttccgc caccccgttc
     3241 atcgggttgt tcggcactgt gtggggcgtc atggatgcgt ttcaaggcct cgggcaggag
     3301 ggcggagcaa gcctgcgtgc ggtcgctccg ggaatttccg aggcgctgat cacaaccgct
     3361 ttcggcctgg ccgccgccat ccccgccgcg attttctaca actacttcct gcaccggatc
     3421 aaagaagtgg gatcgcggat ggacgacttc ggcctggagt tcgtgaacct ggcggagcgc
     3481 agtttcggag agtaacggga tatggcattt agccctcgca acgggcgcgg ttccggcgcc
     3541 ctggccgaaa tcaatgtgac cccgttcgtg gacgtggttt tggtattgct gatcatcttt
     3601 atgctgacgg cccacgttat ggaatatggc gtcgaggtgg acgtccccag aaccaagcag
     3661 gtggccaaca cgtccaaaga gttgccggtg gtcaatattt ccagcaaggg cgagctctac
     3721 ttgaactcgg agccggtgaa tatcaacgac ctggtcaaga agctggagcc tatgctgggc
     3781 aaagaccgcg cggcatatct ccgttgcgat aaggacgtga cgtgggaaat ccccgtccag
     3841 gtgatggccg aactcggcca cgcgaaggtg caggtgagcg tggtcaccca gccgctcgag
     3901 cagttgccga gacgaaaatg accaggcatc tggacgtgct cgagcagagg gaaagcctcc
     3961 gcctcccttt tctggagtct ctgggggtgc acctgctggt cctcggctac gccgcctttt
     4021 attcctggat ggtcgtccac aaccgcgtgc agttcggcaa tccggacgcc atggcgggcg
     4081 gagcggttcc aatcaacatt gtgaacagca ttcccatggc gcccgcccaa accgtctcgg
     4141 aaaacccagt ggccaatgac acccgctcca gcgtccctgc gccgcccccg gaaaaagcca
     4201 agtcccgcga gccggtcgag caggccgacg aaacggccgt ccccctgcct acggccaaga
     4261 ctccaaaagc ctccaagcgc caggcgcccg agaagaagtt tcgcccctac gtgcccagcc
     4321 gcgacaacca actgtacgcc atgaaaggaa tgggcgttaa tacgcccacc tatagcggct
     4381 tgcaatctga aaacttcgga gtgggggtcg gcgtcggcgc cggcagcccc ttcggggcgt
     4441 atttcgggtg gtatgccgag gccttgcaga gacgcatcgg cgagcaatgg caaaaagaat
     4501 tgatgcagtt ggatcagcgg attagcagcg tgccgcgaac tgtggtattt tttgaaattc
     4561 agagggacgg cagccttcga aacattcgac tcgttcagtc tagcggcaac tccgctgtgg
     4621 attacgcggc tttgcgggcc gtcaacgaat caaatcctgt gccgccgctg ccctccggcc
     4681 tggcgaagaa ttacgtttcc accgaggtct ggtttcaaat aaagcgatga aagatcaacg
     4741 agtcgcgggc gctctcggaa ccctggtact cgcaggtctg gtaatcggca tcgtcagccg
     4801 ggcgcaacag cggccggaca tcacgattcc cgtggtcggc gagcgggccc ccatccgcat
     4861 tgcggtcccc gatattccgg ggtccggggt ttcagccgac gtcaaccggg tcttcaacga
     4921 gacgctgtgg aatgacctgg aaaattccgg cgtcttccaa atggtgtcca aaagcttgta
     4981 tcccactgaa gtgccgcaaa agccggagga ctttaagccg ccgcttccgg cgccggccgg
     5041 cccgcagcgg cggatgcgtc cgggggaaac tccgcctccg ccgcccgccc ccatccggca
     5101 ggggccctgg ctgaccgact ggtcgcagcc gcccgtgcag gccaatgttc tcgccttcgg
     5161 aaacactgcc gtgcagaacg ggcagttcac cctctccggg tggctgtttg atgtcaccaa
     5221 gaacgacacc gcttccgcct acgtattcgg taagcgctat ttcagctcca atgacgccaa
     5281 cggcgcccgc cttgtcgcgc acgacttctc caatgacatt ttgcaaaagc tcggcgccgg
     5341 ccaggggatt gcgggcaccc ggatttattt cgtttccaac cgcaccgggg gcggccataa
     5401 agaaatctgg gccatggatt atgacggcaa caaccaggcg ccgattaccc ggtacaactc
     5461 gatctgcctt agccctgatg tgagccccga tggatcgatg atcgctttca cgaccttcgt
     5521 gcgcggccgg ccggagattt tcattcactc cctcgaaacc aaccgcaggc tgacgttcta
     5581 taaccaggcg gcgtccctga acgccacgcc cagtttttcg ccggacggaa agcggctggc
     5641 ctatgcttcc tcagccagcg ggcacgcgca gatttatgtc tccaatatcg atggcagcgg
     5701 cttccgcaga atcactttct cgcaagcccc ggaagtcgag ccgaagttca atccgaaaac
     5761 cggcgctcag attgtttttg tctccggccg gtccggacag ccgcagatct acttgatgga
     5821 ctcggatgga acgaatgtcc aaaaactaac gcccaatatg aatgggaatg gcgacgccgt
     5881 caatcccgct tggtcgccgg acggccagcg gatcgccttc tcctggacgc gcggttttga
     5941 gccgggcaac tataatattt ttatccagga aatcgccacg cgaaacatca cccaactgac
     6001 ctacggagcg ggccgtaatg agcatccctc ctgggcgccg gatggccggc atattgtttt
     6061 ttcttcggac cgttccggaa gcgtgcagat ctggacgatg ctggcgaatg gcacccaatt
     6121 gaagcagtta accacacagg gccggaacga aatgcccgtg tgggcagcaa agtagcagcg
     6181 acccaaggga aaggaattcg acgagcatcc attcagcatc tgactcgtcc tgaagcaata
     6241 tctaggaggc gatagaaaga atgcgacgca agcgagagtt gatcgtgatt ttgctgggcc
     6301 tgtgcctggc gctgttcgcg gcgggttgta agaagaaagc gccgccgccg ccaccgccac
     6361 caccgccccc agctcctgcc cctgtccagc cagcgccgcc gccggcgcct accgttacgt
     6421 tcagcgcgga acccagttcg attgagcggg gccagtcggc gactctgcgc tggagctcga
     6481 ccaacgcgac ggaagcctcg atcgatcacg gcatcggaac cgtgggaacc agcggtacac
     6541 gccaggtttc ccccgctgaa tcgactacct ataacatcgt cgtcaaggga gaaggcggcg
     6601 aagcccgcgc cagcgcttcg atcactgtgc gcgcccccgc gcctccgccg ccgccaccgc
     6661 cgccgcggag aagtttcagt gaggatctgg caagccgcgt ccaggatgcg tatttcgact
     6721 tcgataagta cgatatacgc cctgacgccc ggcaagtgct cacatcggac gccgacgcgc
     6781 tcaagtcgct gttcaatgag ttcaacaccg cgcatgtcgt gattgagggc cactgcgacg
     6841 agcgcgggtc ggcggaatac aacctggcct tgggagaccg gcgggccaac tccgccaagg
     6901 agtttctaca agagctcggg gttccggccg accgcctcag cgtcatcagc tatggcaaag
     6961 agcggccgca atgcacggag cacaacgagg aatgctggca gaaaaaccgc cgcgtgcatt
     7021 tcacggccgg tagctgatcc gcgccgggag cggcaggcgc cggagcttca cggggactcc
     7081 ggcgcctgaa acacttttgg gttaaaatga tttatcggag gtctgctgtg ccgcgccgat
     7141 tactttttct attcctgctt gctgctttgc ccgcttttcc ggtcgccaaa gaaatcttgc
     7201 agatccaacg cgatatcgcc ctgctgcaag atcaggtgcg gaatctgcaa agcgcctttg
     7261 acaagcaact ggcggtgaca caacaactgt tgaatcagaa tctggacgcc aacaaccagc
     7321 tttccaaccg cctcgccgtg ttggagaaga gtctccagga tcaaggcaag gtgctggtta
     7381 cccccctcgc taataccaat gcccgcgtgg acgctctggc cggccaggtg cagggtttgc
     7441 gcgatacgct ggacgaaatg aattcccggc tctcccgcct ccagcagcag atggtcgaca
     7501 taaagaacat cgtcagcagc gttcccgctc ccgccgctcc ggcaactggc gtcccagggt
     7561 ctacggtcgc cccgggcgct cccgcggttt cggccgagaa gctattcagc gacgccgtgc
     7621 gcgattacca gggcgggaat ttcgccatgg ccggcccgga atttaccgct tacattcagc
     7681 agtatcccaa cacgccgcag gccgccgagg cccaatatta cctcggagac attttttacc
     7741 agcagaagca gtacgatcag gccgccgaag cttttgatca ggtgctggag cgctatccgg
     7801 aaggcccgcg cactcccgac gcgcagtaca aaaaaggcat gagcctgctc aatcagggca
     7861 agcgggatgc ggcggctcgc gagtttcgcg gcgtcatcag caaattcccc gattccacag
     7921 ccgccagcgc tgccaaagag gccttgcgaa atctgggcct ctcagcgcgc tcagctccgg
     7981 ggaaatccac caagagccgc aaaaaatagg cgatcttttc ccgcccccgt ccccgtcctt
     8041 ttccgcttcc gcttaccagc gccaatcggc gacgaagacg ttgaactcgt accgctgctt
     8101 ggcattgcgg tccgagatga agacgatttg cttgccgtcg ggagagaact cgggaaatgc
     8161 cgtgaaaccg ccgaacgagg tcacttgctc cacgttgttg ccgtcggtat ccattaggaa
     8221 cagctcaaac tgccggctgt cgcagtggtt cttgttggac gaaaacagaa tcttgcggcc
     8281 gtccggactg aaggtcggcg caaagctggc gcagccataa ttggtgagct gttgaacatt
     8341 tttcccatcg gcatcggcca ggtagagctc cattttcatc ggagtggtca ggttgtcggc
     8401 aagcagcttc ttgtaggcgt ccagcgcctc cggcgtcttc gggtgattgc cccgccatac
     8461 gagctttttt ccatcgcggg aaaagacgcc gcctccgtcg tagccaacct catgggtcaa
     8521 ctgcttcttg ttgctgccgt ccagattcat ggaccacaga tccagatcgc cggaagccat
     8581 agaggtgtaa accagctttc ggtctttcca attgaccgtc acttcggcgt cgtatcccgg
     8641 ctctttcgta agttggcgga gaatctggcc tctgtcggtt gctacgaaga gatcgtacgt
     8701 tggataaacc ggccagacgt atcccttgct gcggtccggc ggaggcggac agcccgcgcc
     8761 gcccagatgg gtggaggcgt atacgaaggt cttgttatcc ggcaggaaat atccgcacgt
     8821 cgtgcgcccc ttgcccgtcg aaaccatatg cacgcccgac ccatcggcgt tcatgatgta
     8881 aatctggtcg cattcccggc cgtcccgagt ggattggaaa accagccgct tgccatccgg
     8941 agaccagtag gcctcggcat tcgatccgcc gaaggtgagc tgccgtatat tggaaatgtg
     9001 tcctgagtct ttcgcggcag gcgccagcag cgccaaaagg aagagggcca gagctcgcat
     9061 gggtgcatga tagcagtcga ccatcttccg cctgaacaca atatcttggg ccaaatcttt
     9121 aagccacaca aaatacggca tctaaaattc tttacttggg gccgatcgtc cgagtaggat
     9181 agggagcgaa ggaaagcact gtgttgaaat taaagaaagt tgaactcctg ggttttaaat
     9241 ccttctgcga gcgcaccgag atgcatttta acggtgatgg catcgtaggc atcgtgggtc
     9301 cgaacggctg tggaaagtca aatatcagcg atgccgtcag ttgggtgctg ggggaacaat
     9361 ccgcccggct gctgcgggga acgcggatgg aagacgtcat tttcaacggg acccgcgacc
     9421 gcaagcctct gggaatggca tcggttaccc tcaccctgat cgatcccgaa gccttttcac
     9481 cggctgagcc ggaacccgtg gcgcccgccg acgccgcgga tgctccgccc gctggagctc
     9541 cacaaaacgg ctcagccaga gcgcgccggc ggccgggcga acggcccggc gagatcattg
     9601 tcacccgccg gctgttccga agcggcgaga gcgagtactt gctgaatggc cgcctttgcc
     9661 gccttcgcga tatccaggat atcttcatgg gaaccggact cggtcccgag agttacgcca
     9721 tcatcgagca gggacgcatt gggcaaattc taagctcgcg cccgcacgat cgccgggcgg
     9781 tgatcgagga agccgccggg atcacgcggt tcaaaaccaa gaagaagctt gccgaggcga
     9841 agctggaaag cgcccgccag aatcttgcgc gcgtaaatga cattctggaa gaggtcagcc
     9901 gccaggtgaa ctctctcaag cgccaggctg ggaaggcccg gcgctacgaa gagctgcggg
     9961 ccgaaatgaa cgcgaagctc accgtagttc tggcgagccg ccaccgggac atggagcgcc
    10021 tggccgtgca ggcggcgctc gatctgaatc tggcggctga agagtttcga agctgcaacg
    10081 cccgggtcga gcggttggag gccgagttcg gagccgcgca gaaagaaagc tttgacctcg
    10141 aatcccaact gcaacagagc agggaagctt gcgccgccgc ggctcgggaa gcggagagga
    10201 cgcagcaaag gatcgactat caggcccggc tggccgagga gaatcgaacc agaatcgggc
    10261 aggcggaaac ggaaatcgcc ggcattgaat cccggctgga agggcttcgg aaggaaatag
    10321 ccgctgagcg cagcgcggcg gatcttgtgt ccgctgagcg cagcgctgtg cgcgagcaaa
    10381 tagagaccgg gacgagcgaa ctggacggca tccagagcca tattcgcgag cgcgaaaggg
    10441 agcaagagga gctgcggcga agagtcattt gcctcctggg ccaggcctcc tccttgcgca
    10501 atcagcttgc gcaaatcgat gagttcctgg cgggccttga gcggcagtcg gcgcgggccc
    10561 gcagcgaaga ggcggccgcg aacgaagaaa tcgccgccgt ctcggcctat cgcgattccc
    10621 atctggcccg cctgcgcgag cagcagctac agctcgagtc actggacgag agccgccggc
    10681 gcctggaagc gagcctggca gaggcgcagc ggcaggcgca ggcccggcgg gaacaggtgc
    10741 agcttctgaa ggaagagatc tcccgcatcc gggctcgccg tgaatcactc gaagaaatct
    10801 tgtcccaccg cgcgtacacc accgagacag tcaagagcct gttcgccgct atggagcagg
    10861 gccgcatgga ggggtttcgg cccctgggca tcttcgccga cttcatcgag gttgatgccg
    10921 agtatgagaa ggccgtcgaa ggctttctgc gcgaggagct ggagtatgtc gtagtaagca
    10981 attgggaaga agcggagcgc ggcatgcggc tgctgcgttc cgaaatggaa ggtcatgcga
    11041 cgtttctggt ttaccccggg gcccagccgg aagcaggcgc cgccggggag gcgcaaagca
    11101 gtccgcttgc ctcccagccg ggggttcgtg ggccgctgcg cgcgcatgtg cgctttaaca
    11161 acggcctcag ccacgcggct tcggcgctgc tgcctcgcct cgatcgatgt tacctggccg
    11221 aaaacgagga gacggcgcgc catcttgcct gccagcatcc ggatttctat tttctgcttc
    11281 ccgagggatt gtgctaccac ggctgctctc tcagcggcgg ccgcaagacc tcgtcggggc
    11341 cgctggccct caagcgagaa ctgcgtgaga tcatgccgcg gctggctgcg gccatgcagc
    11401 aactggaagc cggctccgcc gcgctcagcc aggccgaagc ccaaatcgcg tcggaaacgg
    11461 cggcgctgga ggctttgcgt caggaaatgc agaatcggga gaaagggact ctggcgtcgg
    11521 agcacgaact gcgccagatc cacgaccgct tgcatcgcgc gaacgcgcgg ctctcggcgg
    11581 cgcgcctgga aatagaccgg ttccgccagg agagcgagcg ggctgccgta gaggctgacc
    11641 gctgccggag ccaggcggcg gatctcgaaa accagcagcg ggaagcggag gaaatgctgg
    11701 cggccatccg ccgggctgtc gccgaagacc aggcgcgcgg agcgaaagcc gccgaaaagt
    11761 taagcgaatg gcgtgcgcag ttggcggctc tcgaggagcg gaaaaaggcc gcacaagccg
    11821 ccgcggcgcg gcttgagcaa accttcgccg gtgaagctcg ccgccgcggc gagatggccc
    11881 agcagatcga ggcgtgggcc gccgaacgcg agcgcctcct acgggagaat cgggatctcc
    11941 aggggcaatt ggaatcagcc gccgcccggc agtgtgcgtt gcagcaggaa gggcgcaacc
    12001 tcgaagcggc tctcaatcag cgccgttcgc gcatggcgga aatcgaggag tcgctgaaga
    12061 ccgcaagaca gcagctcgaa gcggcgcgcg gccggcgttc cacaattgaa attcagctcg
    12121 tgcggttgca gtccg
//
LOCUS       DASREK010000289        16890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2285824, whole genome shotgun sequence.
ACCESSION   DASREK010000289 DASREK010000000
VERSION     DASREK010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 16890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16890
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285824"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFA54_15620"
     CDS             complement(<1..187)
                     /locus_tag="VFA54_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52297.1"
                     /translation="MRRDSKIFLAMAALLFTASLSAQLSIPEIPYDSTPNLLKLPEHI
                     YLGEAVGVATNSKGNIFV"
     gene            complement(242..1309)
                     /locus_tag="VFA54_15625"
     CDS             complement(242..1309)
                     /locus_tag="VFA54_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HZS52298.1"
                     /translation="MRRVLQSCFFVVALLAATLPPAQAQNLTQAQLNLKAKAQNVPEI
                     PYELAPNFLKLPPDLYFGESIGVATNSKGHVFVYDRGHETRLFEFDQNGNFVRFIGEG
                     LYGFAFAHVVRVDSQDNIWAVDEGTNMVIKFNPQGRVDMLLGRRPEAHGLQASPPPGT
                     PEPPARPYVFNRPTDVTWDPAGNIYVSDGYGNARVVKYDKNGRFIKSVGTLGTQPYQF
                     NTPHSIAADAKGNVYVADRGNRRIQVFDSDLVLRAIYDQVGTPWAVCITPGPHQYLYS
                     SNSNPNNSSPEISAVTGEIYKMELDGTILGKFGKPGKLPGEFSTVHEIDCRNENEVTV
                     AEITGWRVQRFKLYPKTAASR"
     gene            complement(1644..2525)
                     /locus_tag="VFA54_15630"
     CDS             complement(1644..2525)
                     /locus_tag="VFA54_15630"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS52299.1"
                     /translation="MKRTVICSFLAALLLGVPAAAQWGSMVPRPQGKLYNSAKQKLLD
                     GKQIFSYTISHLDPDLYCQAAAHFDFIFFDMQHSTMSWADIEKMIAACPGVGAPMIRL
                     PDEFESTIQHATDIGAIGVIEPTVDTVEKAEAVAKFAKYPPEGRRSLGGGQAPRIWGA
                     FGNYRQTINDNMLVVVMIETPVGVANAYDIARVRGVDVVLVANTDLGNFSGYAPDSPD
                     YQELVKKIHDATLKAGKFLGATNASYAKGRPDSADFRMLQGGPSFDGWQPPARAGRGG
                     QGAGRRGGAQSSGATPQ"
     gene            2936..3499
                     /locus_tag="VFA54_15635"
     CDS             2936..3499
                     /locus_tag="VFA54_15635"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HZS52300.1"
                     /translation="MRVQIRLKIAGPFFALCTLVVAFAGAGFAAQNIQPLDVKLGLWE
                     STTVRESSGFPPIPPEALARVTPEQRAKIEAAAKASASRGPKTETRKSCLTKEKLNKA
                     LTFGQSNNDNCKATVVTSTRTKEEIHFECAGEGMKGAGDMHIEAINSENVKGSIQMNA
                     AGGGNAMKTSVNFTAKWLGPSCADVKD"
     gene            complement(3575..5371)
                     /locus_tag="VFA54_15640"
     CDS             complement(3575..5371)
                     /locus_tag="VFA54_15640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HZS52301.1"
                     /translation="MSGPIQTLGQLFLASVARDKSDYILFRREGKFRPISAKEFLRRV
                     VRLHLGLRSLGIAPGDRCALLSENRWEWAAADFAMMTAGVVSVPLYPSLPPEQLHYML
                     ENSEARAIFTSNEAQWSKIEAIRRRLPALDSIIRMDGQPGGPGSRVFPFGQFISPGPL
                     TAEEGKKFENAIASVQPEDLASIIYTSGTTGTPKGVMLTHRNLVSNVLDAEPDLGPRD
                     VALSFLPLCHIYQRMVDYACYLHGVTVAHVDPIAEVPEALEKVRPTVLSAVPRFYEKL
                     YRRIKETADAAPAPRRQLFRWAVETGFESVSYRLEGRPMPLGLRLRFALADRLVFSKI
                     RRRLGGRVRLLTSGGAALPRQVAEFFYAVNLPILEGYGLTETSPLIAASRPERMRFGT
                     VGKPIRNVEVRIAEDGEILVRGPNVMRGYYKMDQETAEALADGWFHTGDIGSFDADGF
                     LTVIDRKKDLLKTSGGKYIAPQPIENRLRNQPYIADAVVIADNRRFPSALIVPDFDRL
                     GHYAREHGIADNSPSDLAKNPKIVALLEQQVAEACASLAHFEQIKKIAVLDHEFSLAA
                     GEMTPTMKVRRREVERRYKDEIEKLYEEAAVP"
     gene            5479..5853
                     /gene="rplU"
                     /locus_tag="VFA54_15645"
     CDS             5479..5853
                     /gene="rplU"
                     /locus_tag="VFA54_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZS52302.1"
                     /translation="MTRPVRPCYYKRLCDTYRSVEMFAIIETGGKQYRVSPGDQIRVE
                     TLPGELNSPVEFSRVLAVCKDGGELLAGDEVRAARVTGAITAHGRADKITVFKFKRKK
                     QYKRKIGHRQNYTQVRINEVVL"
     gene            5853..6137
                     /gene="rpmA"
                     /locus_tag="VFA54_15650"
     CDS             5853..6137
                     /gene="rpmA"
                     /locus_tag="VFA54_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZS52303.1"
                     /translation="MAHKKGLGSSRNGRDSQSQRLGVKRFGGQLVTGGSILVRQRGTP
                     IRPGQNVGRGKDDTLFAKITGIVEFADRGRHGKFVHVREPNGAELGRANS"
     gene            6303..7325
                     /gene="obgE"
                     /locus_tag="VFA54_15655"
     CDS             6303..7325
                     /gene="obgE"
                     /locus_tag="VFA54_15655"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZS52304.1"
                     /translation="MFIDEATIFVKAGDGGNGCLAFRREKYVPRGGPSGGDGGKGGDV
                     ILQASERHNTLIHFRFNPEHRAERGRHGEGSNRTGRDGQDLVLRVPVGTLVYDAETNE
                     LLHDFAQPDESVVIARGGRGGRGNARFATPTHQAPREAEAGCPGEERRLRLELKLLAD
                     VGLVGFPNVGKSTLISRISAARPKIADYPFTTLEPNLGVVSLGEYDSFVVADIPGLIG
                     GAHLGHGLGTRFLRHIERTRLLVHLIDVSEASDRDPVDDFRVIAGELASFNPQLAEKP
                     VLVVASKIDAAQHPERIEKLRAFCLGRDLPFFALSAVTGAGLDQLKFHMAERVREARL
                     QQTATA"
     gene            7322..7948
                     /gene="nadD"
                     /locus_tag="VFA54_15660"
     CDS             7322..7948
                     /gene="nadD"
                     /locus_tag="VFA54_15660"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZS52305.1"
                     /translation="MNLAIFGGTFDPVHNAHLEVARAARREYFLDRILFVPASQPPHK
                     PGRCGADYEHRYEMVRLACAEHPAFEPSRLEAPGRDRGRSYAIQTIRRVAAYLTPHDR
                     LFFLIGADAFAELTSWYRWRLIVQDVEFIVVNRPGFALDESTVPPGARVHWLRSVNSP
                     ISSSEIRHRLQTGQSIKGLVPAAVARYISRYQLYRPARAEKRRRSARA"
     gene            8098..8652
                     /locus_tag="VFA54_15665"
     CDS             8098..8652
                     /locus_tag="VFA54_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52306.1"
                     /translation="MKSFSPAVFLCLMLIGVIGAYWLWLGTAGGGATFSEVVERAGQK
                     VDSLWPKAPEGPPPAPITEETLHPKKTVVPVHRAQKKVEPETPEPSETGAVAAANTPP
                     APLEPPPAPKVERPPSRFPEASEIPVGTERFRLLADFGRPAMITTSMSDNRFVETFVY
                     FRGDTATYVQVQDGRVIAARTGSY"
     gene            8879..12004
                     /locus_tag="VFA54_15670"
     CDS             8879..12004
                     /locus_tag="VFA54_15670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52307.1"
                     /translation="MNTVRLVLLLLAAAGGAAAAPFGTAYPLLGEFSDLALDESRSVL
                     YLANFTASRIDVFSTATNALQQPIQLGINPSAIALSPDNRNLLILNFGSPSLFLLNLD
                     NRSVQTTALPDLPPPQTNIPNLPRAAAFGSDGTAIIVTSGEVLKFDPATGQVTVLLQN
                     LNVIGGTPTPEPATPAEIVNARMAASADRNTLFIISQTTNLAYAFYFSVPDQKFISIG
                     CTTPVVTPTTFASVAPNGSLLFGGGTLFDRQMRILADFVPSGVLPIKVAPANPTAPPP
                     PPNVVIGASEFSRDSSTIYASFFDSSSLSAAPFLYVLDSDNLTIRDRILLPDQLAGKM
                     VSDSAGAKLYALSQKGLTIIPIGSLNSAPRLTASSDTIVFRFNVCNKLTATATFDVVN
                     QGPGSVDFTLSTNMPGLSLSQTSGTTPAHITATFDPNQLFNVKGTTLGLINVSSNGAV
                     NIPGSIRVLANLQDTDQRGTIFVQGGTLRDLLIDELRQRFYILDSSRNQLLVFDLNDF
                     SLKATVRTGYFPLHMAIAPDQKTLLVTNAQSETISKIDLNTLQPTGFVYCPCRAYPRS
                     IAVSTNAILVTTAIDRQETVTNSSNQPVQVIVTEGRMNRVDLTAGVARELATMGIFTN
                     NLSPKSLLTATPSGSRILIAEDVPGQGSTATGGIAKIYDAQSDSFILARAISSAPLKG
                     DAAASDTGLYNAGAVLLGPSLAPLTQFNDAPNLQNGFVFVGGGIVRTLGPATGGGPGL
                     ITRVDPTTLQSVHPVKLAEAPLQLLDDQPLRRSLAGASDGSKFITLSSSGFEVIPGSF
                     DAFLPPPAISSVTNSATFSGPVAPGSLISVFGNNLAPLTTSAGSVPLPTFLADACITV
                     NNLPIPLLYLSAGQINGQLPFEVNGAASMVIHTPGGVSDPFNFQVSAAAPGLFSSDFS
                     GQAPTPIPIILRAFNQQPVTPSNPAHRGDILVMFGTGFGNVSPGLGSGQAAPVGALFN
                     TTLTPTVTIGGVQAEVLFSGLAPGFVGLNQLNVKVPLNAPLGFQVPLQITSGGVSTQT
                     FLVRVQPEMEQ"
     gene            12028..12711
                     /locus_tag="VFA54_15675"
     CDS             12028..12711
                     /locus_tag="VFA54_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52308.1"
                     /translation="MRFVPVLVLLCGAAGLLRAQSSGAWKMGGPDDRWEVSGIAGGSL
                     YHDVSASATNPLRKASVGFFNGVAVGAVLGQLGGQHWGGEFHYLFQTNNMRLRAGAGE
                     TGSASFSAKSHAFHYDALFYFTRRDARIRPFIAGGPGLKVYQATGQEQAFRPLSDLVV
                     FSHENDTKFMVSAGGGVKVKVYRGALVRFDFRDYATGIPKSFTAVPGVKLSGQFHNFV
                     ATGGIGVTF"
     gene            complement(12751..13032)
                     /locus_tag="VFA54_15680"
     CDS             complement(12751..13032)
                     /locus_tag="VFA54_15680"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HZS52309.1"
                     /translation="MGALSLFIYLQILDFLTTILFLKMGLFEASWVVAALIRWSPILG
                     VLAAKLGTVTLGFIAVRYHKDRVMRLANLGYCGVIAWNLICMILAKQPA"
     gene            13215..15023
                     /locus_tag="VFA54_15685"
     CDS             13215..15023
                     /locus_tag="VFA54_15685"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HZS52310.1"
                     /translation="MPPELFAGSLVERPMAGKSLCASAAIHAHLILLVLAWRFIGPTQ
                     KLPYKPRTYEVHIVRYSLPEPLLFAASGRQGDGPGGGSRGAAPGRGTAPALPGAPRLK
                     IDMPVPALPQREIVAAEPPGLAPPKPAFRIPEKAPVTHRDLIIQPEFKPDIRITGKVD
                     LPNLFLWSQREIRPPVRQLKTFVPGEIQRHPRSPQALPDAVPKLELPNQEVKVTDLKI
                     SSAPQVQRPALPVFAATTSPVRIPRKTLEAPSELPVTALPPGEPVNLLAIMDRPAPPS
                     PSYSTEAGNRFPQAETEGGNDAGSSKSSNPGPGPRGASASAEGAAAGPAGSGTGAANG
                     TPGGMGSSGRGGSAAGPGSGGTGRGAAAGSGRGLGTGAGGGAAGAGAGRGRGSGSGSG
                     AGAGAVAGMGGRGSGSGSGIGAGGVGSGSQPGGAGTEIAAVHLGSNSVAIRTESPNTS
                     SFDVVVVNQNAREILPESQGILTGQPIYTVYMSVAGSRREWILQYTVPNSREPVVQTS
                     PNSVQLGAATPIRAPYPLRKASLQLPSQAPVEGRVVVYGDISDKGALENLRVIRGVRS
                     EIDAAVLACLRQFVFRPAVRDGVPVPVEALFGIPLN"
     gene            15247..15726
                     /locus_tag="VFA54_15690"
     CDS             15247..15726
                     /locus_tag="VFA54_15690"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HZS52311.1"
                     /translation="MPRIRSKQSRGQALIEASLLVPMIFFLFLAMTNFGFFIYAFITT
                     GNAARAAAQYTANPSFVGQQPIACRVVLREMATLENVFSLGSGQVSTGLWTSSNTCSA
                     APLTVTVAPPTYTEPTSGTPASRVTVTYNTIRLFLLPFMSGQMTISRTATMRLMPAS"
     gene            15745..16353
                     /locus_tag="VFA54_15695"
     CDS             15745..16353
                     /locus_tag="VFA54_15695"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HZS52312.1"
                     /translation="MSRAVFRIDRARAGNANVEFALCAIFVIFLLISIFDMARGLWIY
                     HTLSEAVRDGTRWAIVHGDRYVDPTNPDPLTNRLPGATLGDAVQVIERAAVGIVPSQL
                     NLTFTVAAVGSGGSNVLSEITCKPATAATCTAVTGTCVAAGNVASTSNWPPNGCAQSN
                     LEVGITAQYPYNSMVVMFFPGMKGVQFGKYILGSSAHEAIVF"
     gene            16372..>16890
                     /locus_tag="VFA54_15700"
     CDS             16372..>16890
                     /locus_tag="VFA54_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZS52313.1"
                     /translation="MIRPHKSKELGSVIVMVTVSLLMLLAMIGMVVDIGWAYFERRAA
                     QVAADAAAEAGARRAFIKGNDPSAPNFCLAANDTDVLNDAKAYATINGFTDAGEGGRQ
                     KVTITSGCAPFPDVTNYPNLDRLHILYWVQARITTRQTSLFTGSAGTGYDFSSGATAV
                     AAVLQVPIQGSVY"
BASE COUNT         3272 a         5122 c         5125 g         3371 t
ORIGIN      
        1 acacgaaaat gttccccttg gaattggtcg ccactcccac cgcttccccg agataaatgt
       61 gctcgggcag cttcagcagg ttcggagtcg aatcgtaggg gatttcaggg attgaaagct
      121 gagcagaaag cgaagcggta aaaagcagcg ccgccattgc gagaaaaatc tttgaatcgc
      181 gcctcatgca aacctcctga tgaaggggac gcccgattgg atccggcctc tcgccctggt
      241 gctaccgcga agccgccgtt ttggggtata gtttgaatct ttgcacccgc cagccggtga
      301 tctcggcaac ggtgacttcg ttttcattcc ggcaatcaat ttcgtgcaca gtgctgaact
      361 cgcccggcag cttgcccggc tttccgaatt tgcccaagat cgtgccatcc agctccattt
      421 tgtaaatctc gccagtgacg gcggatatct cggggctgct gttgttgggg ttcgaattgg
      481 agctgtatag atactgatgg ggtccgggag tgatgcagac ggcccacggc gttcccacct
      541 ggtcatagat ggctctgaga accaagtcgc tgtcgaaaac ttggatgcgc cggttgcccc
      601 ggtccgctac gtagacattg cccttggcgt ccgccgcgat cgagtgcggt gtgttgaatt
      661 gatagggctg tgtacctaac gtgcccaccg atttgatgaa ccggccgttc ttatcgtact
      721 tgacgactct cgcgttgcca tagccgtcgg aaacgtagat attgcctgcc gggtcccagg
      781 taacgtccgt aggccggttg aaaacatagg gccgtgccgg cggctcgggt gttcccggag
      841 gaggcgacgc ttgcagcccg tgggcttcgg gccgtctgcc caggagcatg tccacgcggc
      901 cctgagggtt gaacttgatc accatgttcg tgccttcatc gaccgcccag atgttgtcct
      961 gcgaatcgac ccgcacgacg tgggcgaaag cgaagccgta caaaccttcg ccgatgaagc
     1021 ggacgaaatt tccgttttga tcaaattcaa aaaggcgggt ctcatggccc cggtcgtaaa
     1081 cgaagacgtg ccctttggaa ttcgtggcta ccccgatgct ctcgccgaaa tacagatccg
     1141 gagggagttt caaaaagttg ggcgccagct catacggtat ctccggtacg ttctgagcct
     1201 ttgccttgag gtttaattga gcctgggtca ggttttgggc ctgggcgggg ggaagcgttg
     1261 ccgccagcag ggccaccaca aaaaaacagg actgcaagac tcgccgcata ggagcctccg
     1321 aggctacgca ggcgaacgcc acctggcgta gtgtgtctat ggattatacg tgattcgggg
     1381 cccgcggttg caattcttcg atgactgcgg aatcggccgg caagtacaat cggcaggaat
     1441 cgttcgccgg cgaagccgag gcccggcccc tggagccgtt tccggctcct cggactcagg
     1501 gcctcggcgc gagccacgca gatccgtggc tcgagcggat cacactccgc tgacatcctg
     1561 gagcaacggg agccaatccg ccgtccggcg ggcagcatcg tcagtacgcc cgcagtccgg
     1621 cttctcccgg cgaaagagaa cgcttattgt ggggtagcgc cgctgctctg cgcgccgccc
     1681 cgccttccag cgccttggcc gccgcggccg gcgcgcgccg gcggctgcca tccatcaaag
     1741 gaaggcccgc cctggagcat gcggaaatct gcgctgtccg gccgcccttt cgcgtacgac
     1801 gcattggtgg ccccgaggaa cttgccggct ttcaacgtag cgtcatgaat cttcttaaca
     1861 agctcctggt agtcaggcga atccggcgca tatccggaga agttgccgag gtccgtgttc
     1921 gccaccagga ccacgtccac tccgcgaacc cgggcgatgt cgtaagcatt ggccacgccg
     1981 accggagtct cgatcatcac caccaccagc atgttgtcgt tgatcgtctg gcggtaattg
     2041 ccgaaggcgc cccaaattct cggagcctgg cctccaccca ggctgcgccg gccttccggc
     2101 ggatacttgg cgaacttggc caccgcttcc gctttttcga ccgtatccac cgtcggctcg
     2161 attacgccga ttgcgccgat atctgtcgca tgctggatcg tgctttcgaa ctcatcgggc
     2221 aggcggatca tcggcgcgcc gacgccggga cacgccgcaa tcattttctc gatatccgcc
     2281 catgacattg tgctgtgctg catgtcgaag aatatgaaat cgaagtgagc ggcagcctgg
     2341 caatacaggt ccggatcgag gtgggagatg gtgtaactga aaatctgctt gccatccaaa
     2401 agcttttgct ttgccgagtt gtacagcttg ccctgcgggc ggggaaccat cgatccccac
     2461 tgggcggccg cgggaacgcc taacaacaaa gcggctaaaa acgagcaaat tactgttctc
     2521 ttcatcagga actctccttg tcgcctttgt ccatcacagc cgttggaggc atgagaagga
     2581 tcagcgagac ttttagccgg gattccattg ccgggctccc ggtgagtcaa agtatacgcc
     2641 cggccggcgc gaatgcaaac gcctagtttt gcgcccgtag cgggccggca attcgccttt
     2701 tggaacccgg ccgcccgcac ccaactgagt ggccggacgg acgctggggc gaggacgaaa
     2761 cggcgccccc gctgccagga ttgtcgcagc tcgattcggt ttcgctgcgg caaggacggg
     2821 cgagcccggg cgcacagttt ttcatgcccc cggacagatt gcaaaccggc ggcattgttg
     2881 gcattctgtt aaagaccccg gggccggtgc gccagggccg tgcggaggag gttgtatgcg
     2941 agtgcagatc cgccttaaaa tagcaggtcc cttttttgct ttatgcacgc tggttgtcgc
     3001 attcgccggc gcgggttttg ctgctcaaaa catccagccg cttgacgtga aactgggtct
     3061 ttgggagagc acgaccgtac gcgagtcgag cgggtttcct ccgattcccc ccgaggcgct
     3121 cgcccgggtt acgcctgagc agcgggctaa gatcgaggcc gccgccaaag cgagcgcctc
     3181 gcgcggcccg aagaccgaaa cccggaaaag ctgtctgacc aaagagaagc tgaataaggc
     3241 gcttaccttc ggtcaaagta acaacgataa ctgcaaagcc acggtggtga cttccacccg
     3301 gaccaaggag gaaatccatt tcgaatgcgc cggcgagggc atgaagggcg ccggtgatat
     3361 gcacatcgaa gcgatcaact ccgagaacgt gaagggctcc atacaaatga acgcggccgg
     3421 cggcggcaac gccatgaaaa cgagtgtcaa cttcactgcc aagtggctcg gcccgagttg
     3481 cgccgacgtg aaggattgac cgccggctcc ttgcccgccc ggcgcgggga agggtatcgc
     3541 acgaaaccgc gctggatgga attggcagcc gaggtcaagg cacggcggcc tcttcgtaga
     3601 gcttttcgat ttcgtctttg tatcgccgtt ccacctcccg gcggcgcact ttcatcgttg
     3661 gcgtcatctc tccggcggcc aatgaaaatt catgatcgag aaccgcgatt ttcttgattt
     3721 gctcgaaatg agccaggctg gcgcaagcct cggccacctg ttgttcaaga agcgccacaa
     3781 ttttcgggtt ttttgcaagg tcagaagggg agttatcagc aatgccatgc tcccgggcgt
     3841 agtggccgag gcggtcgaaa tcaggcacga tgagagcgct tgggaagcgc cggttatcgg
     3901 caatcaccac ggcgtcggcg atgtagggct ggttgcgcag ccggttttct atgggctgcg
     3961 gagcgatata cttgccgcct gaggtcttca gcagatcctt tttgcgatcg atcacggtaa
     4021 ggaaaccatc tgcatcgaag gagccgatgt cgccggtatg aaaccacccg tcagccagcg
     4081 cctccgcagt ctcctggtcc attttgtagt agccccgcat cacgttgggg ccccgcacaa
     4141 ggatttcccc gtcttcggcg atgcgcactt cgacattccg aatcggcttc ccgacagtgc
     4201 cgaagcgcat ccgctccggg cggctggcgg cgatcaacgg ggatgtctct gtgagaccat
     4261 agccttccaa tatgggcagg ttgacggcgt aaaagaattc ggcgacttgc cgcggcagcg
     4321 ccgccccgcc cgaggtcagc aggcgaacgc ggccccccag ccgccggcgt atcttggaaa
     4381 ataccagccg gtcagccagt gcgaatcgca aacgtaagcc cagcggcatg ggccggccct
     4441 ccagccggta ggaaaccgat tcaaagccgg tctcgactgc ccaacggaac agctggcgcc
     4501 ggggcgcggg cgccgcatcc gccgtttcct tgatgcggcg atagagcttc tcgtaaaagc
     4561 gaggcacggc gctcagcacg gtcgggcgca ctttttccag cgcttcgggg acttcggcga
     4621 tcggatcgac gtgcgccacg gtgactccgt gcagatagca agcgtaatcg accatgcgct
     4681 ggtagatatg acaaagaggc aggaacgaaa gcgccacatc ccgcgggccg agatccggct
     4741 cggcgtccaa tacgttgctc accaagttgc gatgggtaag catgacgcct ttcggcgtcc
     4801 ccgtcgtgcc ggaggtataa ataatgctgg caaggtcttc gggctgcacg gacgcaatcg
     4861 cattttcgaa tttcttcccc tcttcggccg tgagcggccc tggactgatg aactggccaa
     4921 aagggaaaac gcggctgccc ggaccgccag gctggccgtc catgcggatg atgctatcga
     4981 gcgccgggag ccgtctccga atcgcttcga tcttgctcca ctgcgcttcg tttgacgtga
     5041 agatggcgcg cgcctcagag ttctccagca tgtaatgaag ctgctccgga ggcaacgatg
     5101 gataaagagg gacgctgacc accccggcgg tcatcatggc gaagtcggcg gccgcccact
     5161 cccaccggtt ttcggagagc aaggcgcacc ggtcgcccgg cgcaattccc agacttcgca
     5221 atcccagatg cagccgcacc acgcgccgca agaactcttt ggcggaaatg ggccggaatt
     5281 ttccttcacg gcggaacaga atgtaatcgg acttgtcacg ggccacgctg gcaagaaaca
     5341 attgcccgag agtttgaatg gggccgctca cggttttgat ctgctgttgc agtgatcgaa
     5401 agctgccagg cgcagtccgc cgtcgctgcg ccccgagcag cggaaagcat ccgccggcat
     5461 ccgccgcggc aggcatccat gacacgacca gtccggccct gctattataa aagattgtgt
     5521 gatacctaca ggagcgtgga gatgtttgcc attatcgaaa cgggcggcaa gcagtaccgg
     5581 gtcagtcctg gggaccagat tcgagtggaa acgcttcccg gagagttgaa ctctcccgtg
     5641 gaattctcgc gcgtactcgc cgtctgcaag gacggagggg agctgctcgc cggggacgag
     5701 gtgcgggcgg ctcgggttac cggggccatc accgcgcatg ggcgggccga taagataact
     5761 gtcttcaaat tcaagcggaa gaagcagtac aagcgcaaga tcgggcatcg ccagaactac
     5821 acccaggtgc gaatcaatga ggtcgtactg taatggcgca taaaaaaggt ttaggcagct
     5881 cacgcaacgg ccgggattcc caatcgcaga gactcggggt aaagcgcttt gggggccagt
     5941 tggtcaccgg cgggtcaatc ctggtccgcc agcgcggaac gccgattcgt cccggacaga
     6001 acgtgggccg cgggaaagac gatacgcttt tcgccaagat cactggaatc gtggagtttg
     6061 cggatcgcgg gcggcatgga aaattcgtcc atgttcgcga gccaaacggc gcagaactgg
     6121 gccgagccaa ttcataacta ataccgtttt ggtcttgact tcgaacctgc cggagtcata
     6181 ataagtagtg gaaaaggtta ggcggaagac gccgggcggc actccacccg aacggagcgg
     6241 agacccacct gataggtggc ttcagtccgg caacgatctt ccgcctttat ttttgtcttg
     6301 ccgtgttcat cgatgaggcg acgatctttg ttaaagcagg cgacggcggc aacggctgcc
     6361 tggctttccg ccgggaaaag tacgttccgc gcggcggccc tagcggcgga gacggcggca
     6421 aaggcgggga tgttatcctg caagccagtg agcgtcacaa cacgctgatc cacttccgct
     6481 tcaatcccga gcaccgggcg gagcgcggcc gccacgggga aggcagcaat cgcacgggcc
     6541 gcgacggcca ggatctggta ctccgggtgc cggtcgggac gctggtttac gatgccgaaa
     6601 caaacgagct gttgcatgat tttgcacagc ccgacgagtc cgtagtcatc gccaggggag
     6661 gacggggcgg ccgggggaac gcccgctttg ccacacccac ccatcaggcc ccgcgggaag
     6721 ccgaagccgg ctgccccggc gaggagcgcc gcttgcggct cgagctgaag ctgctggccg
     6781 acgtgggttt ggtcggcttt cccaatgtcg gcaaatctac attgatctcc cggatctcgg
     6841 cggcgcggcc gaaaatcgcc gattatccct tcacaacgct tgagccgaac ctgggtgtag
     6901 tctccttagg ggaatatgac agttttgtgg tggccgatat tccgggcctg attggcggag
     6961 cgcatctggg ccatggcctg gggacgcggt ttcttcgtca catcgagcga acccggctgc
     7021 tggttcacct gatcgacgtc agcgaagcca gcgaccgcga tccggtcgat gattttcgtg
     7081 tgattgcggg cgaactggca agttttaatc cgcagcttgc cgagaagccc gtactcgttg
     7141 tagcatccaa gattgacgcg gcgcagcatc ccgagcgcat cgagaagcta agagcttttt
     7201 gtctgggtcg cgatctgccc ttttttgcac tctctgcggt caccggggcg ggtctcgatc
     7261 aactgaagtt ccatatggcg gaacgagtgc gggaggcgcg cttacaacaa acggcaaccg
     7321 cgtgaatctc gcgattttcg gcggcacctt cgatcctgtt cacaacgcgc atctggaagt
     7381 ggcccgggcc gccagacgcg aatatttcct ggaccgcatt ctcttcgttc ccgcaagcca
     7441 gcctcctcat aaacccgggc gctgcggggc cgattacgag caccgctacg agatggtgcg
     7501 cctggcgtgc gccgagcacc ccgcgttcga gccctcccgg ctcgaagcgc ccgggcgcga
     7561 ccggggccgc agctacgcaa ttcagaccat tcgccgcgtg gcggcgtatc ttactccgca
     7621 cgaccggctt ttttttctga tcggcgccga tgccttcgcc gaattgacaa gttggtatcg
     7681 ctggcggctg atcgtgcaag acgtggaatt tatcgtggtg aaccggcccg gctttgcgct
     7741 ggatgaaagc accgtgccgc ccggtgcgcg cgtgcactgg ctgcggtcgg tgaactcgcc
     7801 catttcttcg tccgagatcc ggcaccggct gcaaaccggc cagagcatca aggggctcgt
     7861 gccggcggca gtcgcccggt acatctcgag gtaccagttg taccgtccgg cgcgcgctga
     7921 gaagcggcgg cggagcgccc gggcctgacc gcaagacggt gaccccgtgg cgcttttggc
     7981 ttttcgggct tcgttcgccg tacccttcag aaaattagta ctccaattac ccttcacaag
     8041 ggtgcgtttt cgttctagca tcagctttta gaattttggc ctcgggtccg gcggttcatg
     8101 aagagcttca gcccagcggt atttctgtgc ctgatgttga tcggggtgat cggggcatat
     8161 tggctgtggc tggggacggc cgggggcggc gcaaccttca gcgaggtggt ggagagagcg
     8221 ggccagaaag tggactcact ctggcctaag gcgccggaag gtcctccacc cgctcccatc
     8281 accgaagaga cgctgcatcc caagaaaacc gttgtcccgg tgcacagagc tcagaagaag
     8341 gtggagccgg agacgccaga gccttcagaa accggcgccg tggctgcggc aaataccccg
     8401 ccggcccctt tggaaccgcc tccagccccc aaggtggaac ggcctcccag ccggtttccg
     8461 gaagcgtccg aaattcctgt gggcaccgag aggttccggc tgctcgctga tttcgggcgg
     8521 cccgcgatga tcacgacatc catgagcgac aaccggttcg tggagacttt cgtttatttt
     8581 cggggcgaca cggcgactta cgttcaagtg caggacggcc gcgtgattgc ggcgcgaacc
     8641 gggagttact aattccgcgg ctggaaacgc gatcggcggg ccgcggatct ccgcctactc
     8701 ggagttccta tttggaaaat cggtaccggg gttacccttc ccaaaaacag cttttccgtt
     8761 tagcatggat gatcgcctac gggggcgccc ggctcgggaa aaaggggcgc ggcagcgggc
     8821 gcacagagtt cccatgggag tgctacgcgg gcagtccgcc ggttcgagga aatgggtcat
     8881 gaatacagta cgcttggttc tcttactgtt ggcggccgcc gggggagccg cggcagcgcc
     8941 gttcggcacg gcgtatccct tgctcggcga gttcagcgat ctcgcgttgg atgaaagccg
     9001 gtcggttctg tacctggcga actttaccgc aagccgcatc gatgtcttct ccactgcgac
     9061 gaacgctttg cagcagccta tccaactggg aatcaatcca tcggcgatcg cgctctcgcc
     9121 ggacaaccga aacctgctca ttctgaactt cggctcgccg tcgctgtttt tgctgaatct
     9181 cgacaatcgt tcggtgcaga ctacggctct accggatctg cccccgcccc aaaccaacat
     9241 tcccaatctc ccccgcgccg cagccttcgg ttcggatgga accgctatca tcgtgacaag
     9301 cggcgaagtg ctgaagtttg acccggcgac cggccaagtc acggttctcc tgcaaaatct
     9361 gaatgtcatc ggcggcaccc cgaccccgga gcctgccacg ccggcggaaa tcgtgaacgc
     9421 ccggatggcc gcctccgcag atcggaacac gctcttcatc atcagccaaa ccacaaatct
     9481 ggcttacgcg ttttatttct ctgtccccga tcagaagttc atatcgatcg gctgcacaac
     9541 gcccgttgtc acaccaacga cattcgcctc ggtcgccccg aacgggagcc tgctgttcgg
     9601 cggaggaacg ttatttgacc ggcaaatgcg aattctcgct gattttgttc cttcgggcgt
     9661 gctgcctatc aaggtggcgc ctgccaatcc tactgcaccg ccgcctccgc cgaatgtggt
     9721 gattggggcc agtgaattta gccgggacag ttccacgatc tatgcaagct tcttcgattc
     9781 gagttccctt tcggcagcgc cgtttctgta cgtgctcgac tcggataatt tgacgatccg
     9841 cgaccgcatc ctgctcccgg accaactggc cgggaaaatg gtatccgatt cggccggcgc
     9901 gaagctctat gcgctctcac aaaagggcct gacgatcatt ccgatcggca gtctgaactc
     9961 ggcgccgcgg ttgacggcca gtagcgacac gatcgttttc cggttcaacg tttgcaacaa
    10021 gctgaccgcg acagcgactt tcgatgtcgt caaccaggga cccggcagcg tagattttac
    10081 gctttcgacg aatatgccgg gcttgagctt gtcgcagacc agcgggacga cgcccgccca
    10141 tatcaccgcc acattcgatc cgaatcaatt gttcaatgtg aaaggcacga cgctgggcct
    10201 tatcaacgtt tccagcaacg gcgcagtcaa cattcccggc tccatacggg ttcttgccaa
    10261 tcttcaggac accgatcagc gggggaccat ttttgtgcag ggcggcacgc tgcgtgatct
    10321 gctcatcgac gagctgagac agcgttttta tatcctcgat tcgtcacgca atcagcttct
    10381 ggtcttcgat ctcaacgatt tttcgctcaa ggcgactgtt cgcaccggct atttcccgtt
    10441 gcacatggcg atcgctccgg accaaaaaac tcttctggtc acgaacgcgc agagcgagac
    10501 aatttccaaa atcgatttga acacgttgca gcccacgggt tttgtttatt gtccttgcag
    10561 agcgtatccg cgctcgatcg ccgtttcgac taatgccata ttggtgacca ccgcgattga
    10621 tcggcaggag acggtcacga attcgagcaa ccagccggtg caggtcatcg tgaccgaggg
    10681 gcggatgaat cgcgtggacc ttacggcggg cgtggccaga gagctcgcca caatgggcat
    10741 cttcacaaac aatctgtctc ccaagtctct gctgacggcc acccccagcg gctcgcgaat
    10801 cctgatcgcc gaagacgtcc ccgggcaagg aagcacggcg acgggcggca ttgcgaaaat
    10861 ctatgacgcc cagagcgaca gtttcatact cgcccgcgcc atttccagcg ctcccctaaa
    10921 aggcgacgcg gcggcgagcg atacgggtct ttataatgcg ggcgcggtcc tgctaggccc
    10981 ttcgctcgcg ccattgacgc aattcaatga cgctcccaac ctgcaaaacg gcttcgtgtt
    11041 cgtcggcggc ggcattgtgc gcacgctcgg cccggcgaca ggcggcgggc cgggcttgat
    11101 cacgcgggtt gatccaacca ccttgcagtc ggtgcacccg gtcaagctgg ccgaagcgcc
    11161 gttgcagttg ctggacgatc agcccttgcg ccgaagtcta gccggcgcca gcgacggcag
    11221 caaattcatt acactctcgt cttccggatt tgaagtgatt cccggcagct ttgacgcctt
    11281 ccttccgccg ccggcgatca gctccgtcac caactcggcc acattctccg gcccggtcgc
    11341 gccgggttcg ctgatctcgg tcttcggcaa caatctcgcg ccgctcacca ccagcgccgg
    11401 cagcgtgccg ctgcctacgt tccttgcgga cgcctgcatt accgtgaaca atctgccgat
    11461 cccgctgctg tacctctcgg cgggccagat caacggccag ttgccgttcg aggtgaacgg
    11521 cgcggcaagc atggtgattc acacgccggg cggcgtcagc gatccgttca atttccaggt
    11581 ttcggcggcg gcgccgggac tgttcagctc ggatttcagc ggccaggccc ccactccgat
    11641 cccgatcatt ttgcgggcct tcaaccagca gccggttacg ccctcgaatc ccgctcatcg
    11701 cggggacatt ctggtcatgt tcgggaccgg cttcggcaac gtctcccccg gcttgggctc
    11761 ggggcaggcc gcgccggtcg gcgccctctt caataccacg ctcaccccca ctgtcacgat
    11821 cggaggtgtt caggccgaag ttctcttttc gggcctcgcg cccggtttcg ttgggttgaa
    11881 tcagttaaac gtaaaggttc ccctgaacgc accgctcggc ttccaggtgc cgttgcaaat
    11941 cacgtccggc ggggtcagca cgcagacgtt tctggtgcga gtacagccgg agatggagca
    12001 gtagctattt ttcaggagag aaatcgaatg cgctttgttc cagttctggt tctgctttgc
    12061 ggcgctgcgg gacttcttcg ggcgcagagc tcaggcgctt ggaagatggg cggtccggat
    12121 gatcggtggg aggtcagcgg aatcgcgggc ggcagtctct atcatgacgt cagcgccagc
    12181 gcaacgaatc cattgcgtaa ggcgagtgta ggtttcttca atggcgtagc cgtcggcgcc
    12241 gtgttgggac agttgggggg ccagcattgg ggcggtgaat tccattactt gttccagacg
    12301 aacaacatgc ggctgcgggc gggagccggg gagaccggga gcgccagctt ttccgcgaag
    12361 tcccacgcat ttcattacga cgcgctattc tactttaccc gccgcgacgc ccgcatccgt
    12421 ccttttatcg ccggcggacc cggactcaaa gtctaccaag ccaccggaca ggagcaggca
    12481 ttccggccgt tgagcgactt ggtggtgttc tcgcacgaga atgacacgaa gtttatggtt
    12541 tcagcgggag gcggagtgaa ggtcaaggtg taccggggag ccttggtgcg cttcgatttc
    12601 cgcgactatg ccacgggaat ccctaaaagc tttactgctg tcccgggcgt caagctttcg
    12661 ggtcagtttc acaactttgt agccaccggc ggaatcggcg tcacgttcta gacgccggtt
    12721 cgtcgcgacc cctgcccctt ttgcgggagg tcaggcgggc tgcttcgcca ggatcatgca
    12781 gatcagattc caggcgatca cgccgcaata gccgagattc gccaagcgca tcacccggtc
    12841 cttgtgatag cggaccgcga taaaacccag agtcacggtg cccagcttgg ccgccagcac
    12901 acccaaaata gggctccagc ggatcagagc cgcgacgacc caactcgcct cgaacaggcc
    12961 cattttcaga aacaggatcg tcgtcagaaa atccagaatc tgcaagtaaa tgaagagtga
    13021 aagtgctccc attcgttccg ctccgcgatg gccgtattgc ctacccttcc agactacgga
    13081 caagacttcc atcggcaaat ccgagctttg cattaagtgt catcggattt gcgacttaag
    13141 cacacctcag cagaacgcct gattttcctg gagccaagcc ggcgaaaccg tgataagtta
    13201 gaccaatacc gtccatgcca ccggaactgt tcgctggttc actggtcgag cgccccatgg
    13261 cgggaaagag cctgtgcgcc tccgcagcca ttcacgccca cctgattttg ctcgtactcg
    13321 cctggcgatt tatcggccca acgcaaaaac tgccttataa gcccagaact tacgaggtgc
    13381 atattgtacg gtacagcttg ccggaaccgc tgctgttcgc cgcttccggc aggcagggcg
    13441 acggcccggg cggcggcagc cgaggggcag cccccggccg cggaaccgct cccgcattgc
    13501 cgggagcgcc ccggttgaaa atcgacatgc cggttccggc gctgccgcag cgggagatcg
    13561 tggcggccga gccgccgggc ttggcgccac ccaagccggc cttccgcatc cccgaaaagg
    13621 ccccggttac gcatcgtgat ctcatcatcc agccagagtt taagccggac attcggatta
    13681 cgggcaaggt cgatctgcca aatctgtttc tgtggtccca gcgggaaatt cgtcctccgg
    13741 tccggcagtt gaagaccttc gtccccgggg aaatccaacg ccatccccgc agcccccagg
    13801 ctcttccgga tgcggttccg aagcttgagc ttcccaacca ggaagtcaag gtgacggacc
    13861 tgaaaatctc gtcggccccg caggtgcagc gcccggcgct gcccgtcttt gccgcaacga
    13921 cttcgccggt acggattcct cggaagaccc tcgaagctcc ttccgaattg ccggttacag
    13981 ccctgccgcc gggcgagccg gtgaacctgc tggctattat ggaccggcca gcccctccca
    14041 gcccttccta cagcacggaa gccgggaatc gttttcctca ggcggagacc gagggcggca
    14101 atgatgctgg atcgtccaaa tcttccaacc ctgggcccgg tccccgcggc gcctctgcca
    14161 gcgccgaagg cgcggccgcc ggacctgccg gctcgggaac cggcgcagcc aatggaactc
    14221 ccggcgggat gggctccagc gggcggggtg gatcggctgc cggcccggga tcgggtggaa
    14281 caggccgggg cgccgcggcc gggtcgggca gaggccttgg aacgggcgcc ggtgggggag
    14341 cggcgggcgc aggtgcaggc cgcggacgag gatcggggag cggctcgggc gccggggccg
    14401 gcgccgtcgc gggaatgggg gggcgaggct cgggaagcgg ttctggaatc ggcgcgggcg
    14461 gcgtggggtc cggatcccag cccggtggcg ccgggaccga gattgccgcc gtccatctcg
    14521 gatccaattc ggtggcaatc cgcaccgaat cgccgaatac ttcttcgttt gatgtcgttg
    14581 tcgtcaacca gaacgcccgc gagatcctgc cggagagcca aggcatcctg acgggacagc
    14641 ccatttacac cgtgtatatg tcggtggccg gttcgcggag agaatggatc ctgcaataca
    14701 ccgttcccaa ctcccgggag ccggtggtac aaacctcacc gaactccgtg cagcttggcg
    14761 cggccacgcc cattcgggcg ccttatccgc tgcgcaaagc gagccttcag ttgcccagcc
    14821 aagctccggt cgagggccgg gttgtcgtct acggcgacat cagcgacaag ggagcgctcg
    14881 aaaacctgcg ggtaattcgc ggtgtgcgca gcgaaatcga tgcagcggtg ctggcctgcc
    14941 tgcggcaatt cgtcttccgg ccagcggttc gggacggcgt tcccgtgccc gtggaggcgt
    15001 tgttcggcat ccccctgaat taaaataata ccgaacatac ctctaggacc aacggttcat
    15061 ccgctgaaat atcatccctt gttttcagag agataccttc cacagcctgt ttacaatcta
    15121 caggacgatt agtaccaata gagctttgac tctattgaaa ggcatacgtc gtaagtatta
    15181 gattgaacta atcaatcaca ctcggacggc gtgaggtatt tcactggggt gcggcgaggt
    15241 attggtgtgc cgaggattag gagcaaacag agtcgggggc aggcgctgat cgaggcgagt
    15301 ttgctggtcc cgatgatctt ctttttattt ttggcgatga cgaacttcgg cttttttatt
    15361 tacgcgttca tcaccacggg caacgcggca cgggccgcgg cgcagtacac cgcgaatccg
    15421 tcgtttgtcg gccagcagcc gatcgcatgc cgggttgtcc tcagggaaat ggccaccctg
    15481 gaaaatgttt ttagcctggg cagcggccag gtgtccacgg ggctatggac gagcagcaac
    15541 acttgtagcg cggcgcccct tacggtcacc gtcgcgcccc ccacgtacac agagcctact
    15601 tcgggcacgc ctgcttctcg agtgaccgtc acctacaaca cgatcaggct gtttctcctg
    15661 ccgttcatga gcgggcagat gaccatcagc cgcacggcga cgatgcggct gatgccggct
    15721 agctgatgat gagctgacaa aaagatgtcg agggcggttt tccgtatcga tcgcgcaagg
    15781 gcgggcaacg ccaatgtgga gtttgccctc tgcgccattt ttgtcatctt cctccttatc
    15841 tcaatcttcg acatggcccg gggcttgtgg atctaccaca cgctttccga agccgttcgc
    15901 gacgggacgc ggtgggcgat tgtgcatgga gatcgctacg tcgatccgac caatccggac
    15961 cccctcacca atcgtttgcc cggagcgacg ctgggcgacg cagtccaggt cattgagcgg
    16021 gctgcggtgg gaatcgttcc cagccagttg aatctgactt tcaccgtcgc tgccgttggt
    16081 tccggcggca gcaatgtact gagcgagatt acttgcaagc cggcgacggc tgccacctgc
    16141 acggccgtca cgggaacctg cgtagccgcc ggcaatgttg cgtcaaccag caattggcct
    16201 ccgaacggct gtgcgcagtc gaatctggaa gtgggcatca cggcgcagta tccatacaac
    16261 tcaatggtcg tgatgttctt ccccgggatg aagggcgtgc agtttgggaa atacattttg
    16321 ggaagttcag cccatgaagc gatcgtgttc tgagcctcgc gaggtggagt catgattcga
    16381 ccacacaaat cgaaagagct tggctcggta atcgtgatgg tcacggtttc gcttctcatg
    16441 ctgctggcca tgatcggaat ggtcgtggac attggatggg cctacttcga gaggcgtgcg
    16501 gctcaggtgg ccgccgacgc cgcagcggaa gcgggagcca ggagggcgtt tatcaaagga
    16561 aacgacccgt cggcgccgaa cttctgcctg gcggcgaatg atacggatgt cctcaacgac
    16621 gccaaggcgt atgcgacgat caacggcttc acagacgcgg gcgaaggagg gcggcaaaaa
    16681 gtaacgatca cctctggttg cgcgccgttt cccgacgtaa cgaactaccc caacctggac
    16741 cgcctccaca ttctctactg ggttcaggcc cggattacca cccgccaaac ttctttgttc
    16801 accggcagcg cgggcacggg gtatgacttc agttccggcg ctaccgcggt ggccgcagtg
    16861 ctccaggtgc cgattcaggg atccgtttat
//
LOCUS       DASREK010000290         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_964259, whole genome shotgun sequence.
ACCESSION   DASREK010000290 DASREK010000000
VERSION     DASREK010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964259"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..624)
                     /locus_tag="VFA54_15705"
     CDS             complement(256..624)
                     /locus_tag="VFA54_15705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS52314.1"
                     /translation="MMKASVGRRLFPVAGLLALFAVLAPAFPAAKDPPLRSVQGIVTD
                     QQENPIARAVVQLKNMKTLEVKSYYTDEKGAYHFHGLDPDIEYELKAQAGNAISSVRK
                     ITPFDSRREVVINLKLDINK"
     gene            complement(686..949)
                     /locus_tag="VFA54_15710"
     CDS             complement(686..949)
                     /locus_tag="VFA54_15710"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS52315.1"
                     /translation="MIFGTVFQENGFLVRGARVEVSNADHPKQKKEAITDIQGEFAVR
                     VPAGKARYMVEVSAKGFAPDQKMVEIAGDERIDLTFHLSPLGK"
     gene            complement(1050..1238)
                     /locus_tag="VFA54_15715"
     CDS             complement(1050..1238)
                     /locus_tag="VFA54_15715"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52316.1"
                     /translation="MATCPVCDGTFYVDEEEVEEGDLISCEECGAELEVVSTDPLELE
                     AVEEEEVKEDEEEEEMEM"
     gene            1353..1832
                     /locus_tag="VFA54_15720"
     CDS             1353..1832
                     /locus_tag="VFA54_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZS52317.1"
                     /translation="MSSDGSRRYPERPIIGVGGILFQDERVLLVKRGRPPLQGTWSLP
                     GGVLEVGELLADGLRREILEETGLEVRLLKLVEIFERIMRDASGRPEYHYVLIDYLCE
                     KTGGELRAGDDVLSVEWVEQDRLPDYDITSGTADVIRKAWRSRSLRCSRKIQAGPEQ"
     gene            1829..>2131
                     /locus_tag="VFA54_15725"
     CDS             1829..>2131
                     /locus_tag="VFA54_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HZS52318.1"
                     /translation="MNPTAEETLEFLPVVNIVRRYIASPLGAAALDELRPFDTRVPAE
                     GELAAVGEAMEYLRGAEQEGGPAGNRPPGLISFRGIHDVRPAVAKLAIEGAELEPLE"
BASE COUNT          429 a          664 c          611 g          427 t
ORIGIN      
        1 catcggtcgc aggcagattg gagaacgccg gcgccttcat gccaatctcc accgccgagt
       61 tgtacttgcc ggcgtaaagc aaatttgccg cggccccgac cgtcaggagc aaagagcagg
      121 tcacaaaacg cgctgaatgc agcttcatac gggcactcct cgtattttcc gacatcctag
      181 cataggggcg gcgcgcgtac aggcgcccgg ccctaaggca aagacggacg ccgcgcccga
      241 tggttactcc cagctttact tgttgatatc gagcttcagg ttgatgacaa cttcacggcg
      301 gctgtcaaac ggggtgatct tgcggacgct gctgatcgca ttgcccgcct gggctttcaa
      361 ctcgtattcg atgtcgggat cgagaccgtg aaagtgataa gcccctttct cgtcggtgta
      421 gtaggatttc acctcgagag tcttcatgtt ctttagctgg accaccgctc tggcgatcgg
      481 attctcctgc tgatcggtaa cgatgccctg cacgcttcgc agaggcggat ctttggccgc
      541 cggaaaggcc ggcgccagaa cagcgaaaag cgccaggagt ccggcaaccg gaaacagtct
      601 gcggccgacc gatgctttca tcatccaaca ccctcctgtt tgtaccgatg cataccgggc
      661 ggctgccgtt gcgtccggca ggcttttatt tgcccagcgg ggaaaggtga aaagtcagat
      721 cgatccgctc gtctccggcg atctcgacca tcttttgatc gggagcaaac cccttggcgc
      781 tcacctccac catgtagcgc gccttgccgg ccggaacgcg aacggcgaac tcgccctgga
      841 tatcggtaat agcttccttt ttctgcttgg ggtgatcggc gttcgacacc tccactcgcg
      901 cgccgcgcac gaggaagccg ttctcttgaa aaaccgtgcc gaagatcacc gcctccggag
      961 ttactttcga tcttttttgc ccagcggcct cggagcgcca aaccggaact acaaggcaga
     1021 gggcggcaag gaatgcggcc gaagcggcgc tacatttcca tctcttcttc ttcctcatcc
     1081 tctttgactt cctcttcctc aaccgcctca agctccagtg gatctgtgct caccacttct
     1141 agttccgcac cgcactcctc acagctgatc aggtctcctt cttccacttc ttcttcgtcg
     1201 acgtagaagg tcccatcaca gaccggacaa gtagccataa atccccccac agtctattta
     1261 cccaaactga agcaaagttg caaatgcaaa aatcagccgc cgcgcgggcg ggttgcccgc
     1321 atcctgccct tatggtggaa tcgataaggt gtatgtcctc agacggatcc agaagatacc
     1381 cggagcggcc gattatcggg gtcggcggca tcctgtttca agacgaacgc gtattgctgg
     1441 taaagcgcgg ccggccgccg ctccagggaa cgtggtcgct tcccggcggc gtgctcgaag
     1501 tgggagaatt gctggccgac ggcctgcgcc gggaaatcct cgaggaaacc ggtttggagg
     1561 tccggctgct caagctggtg gagatattcg aacgcatcat gcgggacgcc agcggccggc
     1621 ctgagtatca ctacgtatta atcgactacc tgtgcgagaa aacaggaggc gaacttcgcg
     1681 ccggcgatga cgtgctcagc gtcgaatggg tcgagcagga tcgcctgccg gattatgaca
     1741 tcaccagcgg aacggcggac gtgatccgaa aggcgtggcg ctcccgcagt ctccgctgtt
     1801 cccggaaaat acaggccggc ccggaacaat gaatcctaca gccgaagaga ctctggaatt
     1861 tctcccggtt gtgaacattg tccgccggta tatcgcgagc ccgctcggcg cggccgccct
     1921 tgacgagctg cgccctttcg acacccgcgt gcccgccgaa ggggagttgg ccgcagtggg
     1981 agaagccatg gagtacctgc gcggagccga gcaggaaggc ggcccggccg ggaaccggcc
     2041 tcccggcctt atcagcttcc gcggcatcca cgatgtccgc cccgcagtcg ccaaactggc
     2101 catagagggc gctgaactgg agcccctgga a
//
LOCUS       DASREK010000291        15149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_632931, whole genome shotgun sequence.
ACCESSION   DASREK010000291 DASREK010000000
VERSION     DASREK010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632931"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFA54_15730"
     CDS             complement(<1..356)
                     /locus_tag="VFA54_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52319.1"
                     /translation="MTSRCIPPAVLLAAALYAVPPAGYHLLKSIFVPGEGGWDYLTVD
                     ESGRRLYVSHGTQVEVLDIDHGSIVGRIPDTQGVHGIAIAADLGRGFTSNGRTSTITI
                     FDLKSLHPIEEVKSTG"
     gene            570..782
                     /locus_tag="VFA54_15735"
     CDS             570..782
                     /locus_tag="VFA54_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZS52320.1"
                     /translation="MARVRGKVKWFNNAKGYGFIGQEDGPDVFVHFSAIQTDGYKTLQ
                     EGETVEFEITQGQKGPQAENVVKTDT"
     gene            complement(1071..1640)
                     /locus_tag="VFA54_15740"
     CDS             complement(1071..1640)
                     /locus_tag="VFA54_15740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS52321.1"
                     /translation="MDRRSNGLRQFFENIPRDSRLRILDLGGACQANINYIALQGHRL
                     YTEDLLLGLDRIGPVQDPARRSGAAIRFIEENLNFASEQFDGILVWDSLEFLDDELLA
                     ASVGRLQAILKPGGTLLAFFHTQAKGETVCVYRYQIRDHQTLHLQPRFSRPLPRAFNN
                     RNLERLFGNFRSVKFFLAQDSLREVIVTR"
     gene            complement(1852..2328)
                     /locus_tag="VFA54_15745"
     CDS             complement(1852..2328)
                     /locus_tag="VFA54_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZS52322.1"
                     /translation="MSTTPMPKSSEPVGAVPARAGQAHIGKSVIIKGEISSSEDLYVD
                     GEVRGAIELRDHSLTVGPNGKVDANVSAREIIVLGSLNGNVQATDKIEIRKTGSLLGD
                     LSTARIMIEDGAYFKGSIDILKPGQKPDSGKNAAPKPPQASAQPSLQMAGGDSRKP"
     gene            complement(2572..3573)
                     /locus_tag="VFA54_15750"
     CDS             complement(2572..3573)
                     /locus_tag="VFA54_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HZS52323.1"
                     /translation="MKLVLYPPVEAERLDKIVAASDSISVINARDEAEALREIRDADG
                     FYGKLTPDLLAAAGRLRWVQSPTASLEHYVFPELIEHPCQLTNMRGLFSDVIADHVFG
                     YILCFARNLHHYIRHQIIGRWEAVGGESERETFITGPCRASAIDRAHMHLADATLGVV
                     GLGHIGSEVARRGLAFGMRVLAVDPVQTQAPEGVAALWKPERLSDLLAASDFVVIAAP
                     HTPKTEKMFRRTQFRQMKRSAYFINVGRGAIVDLADLTAALQTGEIAGAGLDVFEIEP
                     LPAYHPLWRMENVIITPHVAGYSPRIAERHLAVLLENVKRFSRGEPLRNVVNKAMWF"
     gene            complement(3628..4527)
                     /locus_tag="VFA54_15755"
     CDS             complement(3628..4527)
                     /locus_tag="VFA54_15755"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZS52324.1"
                     /translation="MPGGAVRMMLSRHSAFYTPLLCTLGAGFLVREGIEGSLATHPPA
                     GRQARSLLTNGEVDILQSSVGSSFVLLERGEQNLPVHFAQINQRDGFFLVGRKPDPSF
                     HWKKLEGAALVAEPAGQPLFMLKYCAHRNGVEWSGIHVIDAGHTPQLEAAFRSGTGDY
                     VHEQGPEPQQLEREGLGYVVASVGEAMPPVCFSTLMASRSFLKTDAAKAFMRAYRKAR
                     EWANQAPAAEIACATIRFFPKYSTEVLAEAIARYQRVGCWDGDAGIPWEGFEQALEVF
                     AFNGQITRRYAYHEVAVPPPDLM"
     gene            4567..5811
                     /locus_tag="VFA54_15760"
     CDS             4567..5811
                     /locus_tag="VFA54_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZS52325.1"
                     /translation="MKRRSFLTSGLLTSGAALARAAGAQTVTPRAVRTAPPKMKITRI
                     RYYLSPNPRPSFNQSTNIVTVETDQGLTGIGEGGSKDTIEQCAGIIIGEDPGRIERCW
                     NLMFRHLFYPPGREKLHALGAIDMALWDIKGKALGVPVYELLGGLARDHIECYATAYP
                     NKGSLKESARACIEAGFRAYRTSVADPGGNAPFDSHQIVRKTHQNCVEIREGVGPNGD
                     WALDYHTRLDMPDAIRLSTLIEDLEPYFCEDLVRSENPGVYRQLRPQVKVPIAVGEHY
                     GARWDTNELIEQRLIDYSRVTIPNVGGVTEYMKIAALCETHYIGLIPHFTGPAGEAAL
                     VHCCATFSGPVLMEMTGEGPRNEPHLTQHFDFRNGKLWPNTRPGLGVEFDPSRAQLIA
                     EITEQRRPTPLFHRADGSITNW"
     gene            complement(5835..6983)
                     /locus_tag="VFA54_15765"
     CDS             complement(5835..6983)
                     /locus_tag="VFA54_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52326.1"
                     /translation="MNPLTFAFSRSVLLAIVLSVSLGGPKGRAADIRYEHLADCITRA
                     LELQKGERVLLRYDSDYFAELRGPLESRIRRAGAVVLGPLEYQAKGVPPLGFGEVIED
                     ADVYLWLPLRPDLHEVSPQEREALQRWLEKGGARREIHFHWQEGSVLSDGLPGPHSPA
                     LDQIYQAALDIDYSALSAAQDRAIALLRSGTIHVTTPAGTDLRFRVGSRPFNKQDGDA
                     SPARARAARVRVDREIELPAGVLRVAPLEETVNGTLVIPSARFGSAVARRLRFSIRNG
                     VITSIQADQNLDAARAALAAGGEAALHFREFGLGFNPKLQAPPESPSLAYYGYGAGVV
                     RLSLGDNQELGGNVRGGFTRWFFFPDGTVEVGGRILVRGGRLSSAGTP"
     gene            7098..9014
                     /gene="dnaK"
                     /locus_tag="VFA54_15770"
     CDS             7098..9014
                     /gene="dnaK"
                     /locus_tag="VFA54_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZS52327.1"
                     /translation="MAKTIGIDLGTTNSVVAVMEGGQPTVIANQEGSRTTPSVVAFTK
                     SGERLVGQVAKRQAITNPENTIFSIKRFMGRRYDEVTEEMKMVPYRVVPDGNLAKVQI
                     GDKKYSPPEISAMILTKLKEAAESYLGEKVTEAVITVPAYFNDSQRQATKDAGKIAGL
                     EVKRIINEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGEGVVEVKSTNGDTH
                     LGGDNIDHEVMQWIISEFKKETGIDLSKDKMALQRLKEAAEKAKMDLSTLLETDINLP
                     FITADASGPKHLQMKLTRARFEQMVEPILQRSVGPVKQALSDAGLTPDKIDEVVLVGG
                     STRIPRVQQIVRNLFGKEPHKGVNPDEVVAVGAAVQAGVLGGEVKDVLLLDVTPLSLG
                     IETLGGVFTKLIERNTTIPTRKSEVFSTASDNQTAVDIKVYQGERAMAADNRLLGVFQ
                     LVGIPPAPRGVPQIEVTFDIDANGILNVSAKDRATNNEQKITITSSSGLSKDEVEKMA
                     KDAEAHAAEDRKRREDIEARNRADAMIYNTEKTLKEHRDKISAEDAANIERAIEEAKK
                     ALQQGDSATINAAVDKLTTASHKLAEAMYRAASQAGSSQQAGPQQAQQGPKADGKDNV
                     VDAEFVDVNDQGKK"
     gene            9072..10256
                     /locus_tag="VFA54_15775"
     CDS             9072..10256
                     /locus_tag="VFA54_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZS52328.1"
                     /translation="MPATAKEDYYKTLGVKRNSSQEEIRKAYRRLARKHHPDLNPGDK
                     AAEERFKQVQEAYDVLSDPKKREMYDQLGFYSETGFNAGAGPRTSSGPPPGGFHFSGF
                     DFSDLFGFGNQEGQAWSRRGPEAGGGFSEMFSHLFRRGGSQEPEPQPGTDLEYTVQIG
                     FWDAIRGTQARLNISRFDACGQCGGAGSIGGAKIQCPECQGTGKVRQMAGAMRFELTC
                     PRCNGKGTLSNTCPACHGEGRISRQETVEVRIPPGAEDGSRLRVAGKGNAGTGGASPG
                     DLYITTRVGEHPFFKRTGNDIHVQVPITVSEAALGAKIEVPTIDGQALLKIPPGTESG
                     KKFRLREKGVLNRRTNVRGDQYVEVKVVVPRIQDERSKEILREFARLNPEDPRAGLFV
                     NR"
     gene            10267..10704
                     /locus_tag="VFA54_15780"
     CDS             10267..10704
                     /locus_tag="VFA54_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZS52329.1"
                     /translation="MARKRTSGAYMISVVSEQYDIHPQTLRLYEREGLLKPSRTEGNT
                     RLYTDRDLERLEVILSLTRDLGVNLAGVEIILNMREKMEAMQRQISEFVACLSQQMNG
                     WPIAPESARRALVPLSQRSTPAPPGGASAPAAGRPKSTSRQKS"
     gene            complement(10676..11251)
                     /locus_tag="VFA54_15785"
     CDS             complement(10676..11251)
                     /locus_tag="VFA54_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZS52330.1"
                     /translation="MILIDIDTQIDFMLPAGALYVPGAEHLIPTLARIFEQARERGVP
                     VISSVDAHSENDPEFAVWPAHCVAGTLGQQKVPATLCKHRAVVPNRPGVDLPDGVEQI
                     IVEKQKLDIFSNPTFERLLDRSTDREYAVVGVATDYCVRCAVEGLLAKGRKVRVITDA
                     IRAIDENAGQKLLSEWAERGATVTTSDAMWT"
     gene            complement(11320..12672)
                     /locus_tag="VFA54_15790"
     CDS             complement(11320..12672)
                     /locus_tag="VFA54_15790"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZS52331.1"
                     /translation="MNGLVTDLYELTMAAGYFEAGKAGDRASFELSIRRLPEDRSYIL
                     VAGVQQAIEYLLNLSFTPAEISYLRSLPGFSRVSESFWEYLGAFRFTGDVWAVDEGTP
                     LFAEEPILRVTAPLPEAQIPETYLLAAVSFQTLIATKASRIVQAAAGRPVVEFGTRRA
                     HTAEAGTLGARAAYIGGCVGTSNVEAGFRFGIPVYGTAAHSWTLAYDSEFEAFSRLQD
                     LLGERTIYLVDTYEVETGIKNAIRVERPFWGIRLDSGDLLADSRRARQILDEAGCSRA
                     QIMVSGDLNEHRIAELLAAGAPIDWFGVGTELATSRDAPAMGSIYKLVEIESRGSVRP
                     TAKFSEDKRSLPGCKQVFRFPDRDVLGLADESFPGGAALLRQVVRDGRRISDPPDIKR
                     LRESALERFYALPERYRRFRFPEKYPVIQSAALQELTREVRKWETGEKPEAASRNPAS
                     "
     gene            complement(12763..13677)
                     /locus_tag="VFA54_15795"
     CDS             complement(12763..13677)
                     /locus_tag="VFA54_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HZS52332.1"
                     /translation="MLLRHFFLFISRSAALRRWMERSPAALRLTSRFVAGTRVEDALR
                     VTRQLNQGGIATTLDYLGESVTNLDEAAAAREVYLQLLDCIHAQGLQANVSLKLTQFG
                     IDISEAACRENVAAVARRACELQSFVHVDMESSDYTDRTLALVSDLHEHFPSIGAVVQ
                     SYLYRSEKDLEELIRRKIRVRLVKGAYDEPPSVAFSQKRDVDANFDKLARMLLQAGND
                     PALATHDERLLRAAIDFAASHGIPKDSYEIQMLYGIRRDLQSRYVAAGHRMRVYVPFG
                     DAWYPYLMRRLAERPANVWFVVSNLLRK"
     gene            complement(13720..14922)
                     /locus_tag="VFA54_15800"
     CDS             complement(13720..14922)
                     /locus_tag="VFA54_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS52333.1"
                     /translation="MTSRRNFIGKVSVGVAGTIATMTPAARILGANDRIRLGLIGAGA
                     RGQQILREALACPNTELVAVADVYRRRHEECRLIAPSARAHFDHREMLEDPSIDAVLI
                     ATPQHLHAEHCVAALEAGRHVYLENTMAFTVDQAKRVRLAFQRAGRVLQIGHQACSSG
                     QVEDVRQILATGLVGRITEIHAWAYRNTPHGRPHWARPVYPDMKPENVHWSMFLGEAP
                     QREFDPNRFINWRLFFDYSGGSIYENMSHQAAFWYKALDLRIPRAVTTRGGIYHWRDG
                     REAPDTLHVIMEQPEEMLFTWSSGLANGHLGSSEEALGSDGTIVRSRQVRYIPEKVNQ
                     PRTSEMLGSNRGGTHMQNFLDCIRSGKDPNCPFEIGFCTSIACRMALESYLQGRTVIW
                     DDRREAIV"
BASE COUNT         3167 a         4639 c         4556 g         2787 t
ORIGIN      
        1 tctcccgtgg atttcacttc ctcgatcgga tgcagggact tcagatcgaa gatggtgatg
       61 gtggaagttc gcccgttgct ggtgaacccg cgcccaaggt cggcggcgat ggcgatgcca
      121 tgtacccctt gcgtgtcggg aatacggccg acaatcgagc cgtgatcgat gtccagcacc
      181 tcgacctgcg tcccatggga gacgtagaga cgccggcccg attcgtccac ggtcaggtag
      241 tcccagccac cctcaccggg cacaaagata ctcttcagca gatggtagcc ggccggcggt
      301 acggcgtaca gggcagcggc gagcagcacg gcgggcggaa tgcaacgact ggtcatttgg
      361 agcctcctcg tacagagcga tgcacgaaac tggggccatt ctattcccac aagatgaact
      421 gaagatgaaa ggggcgacga aacgagccac gctgccccga tgcagccggg ccgttcccgg
      481 ctcaaggtgg tgcgcgttga cacattcaca gagccaatct atagtaaatt aagagagtta
      541 aggcaacacg agtcaggaag ggaggtctct tggctagagt tcgaggtaag gttaagtggt
      601 tcaacaacgc gaaaggctat gggttcattg gccaggaaga cgggccggac gtattcgtgc
      661 acttctccgc gatccagacc gacgggtaca aaaccctgca agagggagag acggtcgagt
      721 ttgagattac tcagggacag aagggtccgc aggcggagaa cgtggtcaag accgatacat
      781 aatccttccg taagcacccg gtgagttttt ccggactgct gccgagggcc gccggcgcgg
      841 aagctgccgt ttttctctgt tccgccggga tccacgagga aatctgtggc tttgctcagg
      901 taagccgccc ggcttcgggc tacctgccgg gcaaccctcc tccgaggggc cgccaggaga
      961 attactcgag ccgatcgaag taccccctac gccggcttgg ggccttaggc cgagagaggc
     1021 cttttggcgc actgacccat acttgggact gggctgaggc tgtctgtcgc ctagcgggtg
     1081 acgatgactt cccgcaggct gtcctgggca agaaagaatt tgacggagcg aaagttgccg
     1141 aaaagccgtt ccagattccg gttgttaaaa gcccggggaa gaggacgcga gaatcgcggc
     1201 tgcaagtgga gtgtctggtg gtcgcggatc tggtatcgat aaacgcaaac cgtttcgccc
     1261 ttggcttggg tgtggaagaa cgccaacagc gtaccccccg gtttcaaaat cgcttgcagg
     1321 cgcccgacgc tggcggcgag taattcatcg tccagaaact cgagcgagtc ccaaaccagg
     1381 atgccgtcga attgctcgct ggcaaagtta agattttcct cgataaagcg gatcgcagca
     1441 ccggaacgcc tggcagggtc ctggaccggc ccgatccggt ccagcccaag cagcaaatct
     1501 tccgtataca gccggtgccc ctgcaaggcg atatagttga tgttcgcctg gcaggctccg
     1561 cccaggtcga gaatccgcaa gcggctatcc cggggaatat tctcgaaaaa ctgccggagc
     1621 ccgttgctcc gccggtccat ttccgccggt tgcggagaac gagtaggaga ccggacagac
     1681 cgggtgaact cgttggacag gactcccgaa ccgccggttg gagcgccatt gcccgcgcgt
     1741 aaatcctggc ccggccggaa aaggctccgg aatttctcat gtaacacttc cccacctctg
     1801 cccgcgtggg cggaatcagc cggtctcgcc tggctaccgc cggcctgcca gctacggttt
     1861 gcgcgaatcg ccccctgcca tttgcaggga tggctgggcc gaagcctgag gtggcttggg
     1921 ggcggcgttc ttcccggagt ccggtttctg ccccggttta aggatatcga tgctcccctt
     1981 aaaatacgcc ccgtcttcga tcatgatgcg cgcggtgctg agatcgccca gcagggaccc
     2041 agtctttcgg atctcaatct tgtcggtggc ctgcacatta ccgttcaagg accccagcac
     2101 gataatctct cgcgccgaaa cgttggcgtc caccttgccg ttcggtccta cggtcaggct
     2161 gtgatctcgc agttcgatcg ctccccgcac ctcgccgtct acgtataaat cttcactgct
     2221 cgaaatctcg ccttttatga tgaccgattt gccgatatgg gcctgtcccg cacgggcggg
     2281 aaccgcgccc acaggctcgc tgctcttggg cataggagtg gttgacactg gcttgacctc
     2341 cgaaacggga ctctccggcg gcggcgccgt tctgggcggc gtagccggcg gcggcgtgtt
     2401 ctctttttta ccccacatac cgcgctccac gagttaaagt atccatacta tagctccact
     2461 gcgaaaaaag aaacgattat cctcccaaaa attagtgtga tgatccgcac ggggtcccaa
     2521 gcctgctttt tgaacaggat cgggccgaag gggcttcccg ccgcaggcga tctaaaacca
     2581 catggctttg ttgaccacat tgcgaagggg ctcgccgcgg ctgaagcgct tcacgttctc
     2641 cagtaagaca gccagatgcc gttcggcgat gcggggcgag tagccggcca catgcggcgt
     2701 gatgattacg ttttccattc tccacagagg gtgatacgcc ggcagcggct cgatttcgaa
     2761 gacgtcaaga cccgccccag cgatttcccc cgtctgtagc gcggccgtta aatcggccag
     2821 atctacgatt gccccccggc ccacattgat gaaatacgcg ctccgcttca tctgccggaa
     2881 ctgcgtccgg cgaaacattt tttcggtttt cggggtgtgc ggggcggcga tcactacgaa
     2941 atcgctcgcc gccagcaggt cggaaagccg ctcgggcttc cacaaagcgg ccaccccttc
     3001 gggcgcctgg gtttgcaccg ggtcgaccgc taaaacgcgc atgccaaacg ccagcccccg
     3061 ccgggccact tccgagccga tatggccgag tccgaccacg ccgagtgtgg cgtcggccag
     3121 gtgcatatgc gcccggtcga tcgcactcgc tcgacaaggt ccagtgataa atgtttcccg
     3181 ctccgattcc cctcccacgg cctcccaacg gccgatgatt tgatggcgga tatagtggtg
     3241 aagattgcgc gcaaaacaca ggatgtaacc gaaaacatgg tcggcgatca catcggaaaa
     3301 aagcccccgc atgttggtca actggcaagg atgctcgatc aactcgggga acacgtagtg
     3361 ttccaggctg gctgtcggcg attgcaccca ccggagccgg ccggcggccg ccagaaggtc
     3421 aggggtgagc ttgccgtaaa acccgtcggc atcgcgaatt tcccgcagtg cttcggcttc
     3481 gtcgcgggca ttaatgacgg agatcgagtc ggacgccgct acaatcttgt cgagcctttc
     3541 agcttcaacg ggtggataaa ggactagttt cataacacga ccggggaatg cgaattgaaa
     3601 ccgcgccgtg ggcgcaggcg cgttctctta catcaagtcc ggtggcggaa cagccacttc
     3661 gtggtacgcg tagcgccggg tgatttggcc gttgaatgca aagacctcga gcgcctgctc
     3721 gaagccttcc cagggaatgc cggcatcgcc atcccagcag cccactcttt ggtatcgcgc
     3781 aatggcctcc gccagcacct ccgtgctgta tttcggaaag aaccggattg tcgcgcaggc
     3841 gatctcagcg gccggagctt ggttggccca ttcccgggcc ttgcgatagg cccgcatgaa
     3901 cgcctttgcc gcatccgtct tcaaaaaaga acgcgaagcc atcagtgtgc tgaagcaaac
     3961 tggcggcatc gcctctccta cggacgcgac cacatagccc agcccttcgc gttctaactg
     4021 ctgcggttcc ggtccctgct cgtgcacgta gtctccagtc cccgagcgga aggccgcctc
     4081 caactggggt gtatgtcctg cgtcgatcac atggatgccg ctccattcca ctccgttgcg
     4141 gtgggcgcag tatttcagca tgaacaacgg ctggccggca ggctcggcca caagggcggc
     4201 gccctccagc tttttccaat ggaaggaagg gtccggcttg cgcccgacca ggaaaaagcc
     4261 gtcccgctga ttgatctggg cgaaatgcac cggaagattc tgctcgccgc gctcgagcaa
     4321 cacaaaactt gagcctaccg aggactgtag gatgtccact tcaccattcg taagcaggct
     4381 gcgggcttgt cgcccggccg gcggatgcgt ggcgagactc ccttcaatcc cttccctcac
     4441 gagaaatcct gcgccaagcg tgcaaaggag aggcgtgtaa aaggcagaat ggcgggatag
     4501 catcatgcgg acggcgccgc cgggcatgtt gtcacttcgc tcccaattcg ctatcttaac
     4561 gcacaaatga aaagacgatc tttcctgaca tccggtcttc tgacctccgg cgcggcactg
     4621 gcgcgtgcgg ccggtgcgca aaccgttacg ccgcgggcgg tgcggacggc gccgcccaaa
     4681 atgaaaatca cgcgcattcg gtattacctt tcgcccaacc cgcggccgag cttcaaccag
     4741 agcacgaata tcgtgactgt cgaaaccgat cagggcctaa ccggcatcgg cgaaggcggc
     4801 tcaaaggata cgatcgagca gtgtgcgggc atcatcatcg gggaagaccc ggggcgcatc
     4861 gagcggtgct ggaatcttat gttccgccac ctgttctatc cgccgggccg cgagaagctg
     4921 catgcattgg gagccatcga catggcgctc tgggacatca agggcaaggc cctcggggtt
     4981 cccgtttatg agctgttggg cgggctcgcc cgagaccaca tcgagtgcta cgccacggcc
     5041 tatcccaaca aagggagctt gaaagaaagc gcgcgggcct gcatcgaagc gggcttccgc
     5101 gcttaccgca cttccgtagc cgaccccggc ggcaatgcgc cctttgattc ccatcagatt
     5161 gtccgcaaaa cgcatcaaaa ttgcgtggag atccgcgagg gagtcggccc caacggcgac
     5221 tgggctctcg actaccacac ccgtctggac atgcctgacg caattcgcct cagcaccctg
     5281 atcgaagatc tcgaacccta tttttgcgag gatctcgttc gatcggagaa tcccggcgtg
     5341 taccgccagc tacggccgca ggtgaaagtg cccatcgctg tgggcgagca ctatggcgcc
     5401 cgttgggata ccaacgagtt gatcgagcag cgattgatcg attactcgcg agtgaccatt
     5461 ccgaacgtcg gcggcgtcac cgagtacatg aagatcgccg cgctttgcga aacgcactac
     5521 attggactga tcccgcattt caccggcccg gcgggagaag cggcgctcgt tcattgctgc
     5581 gccacgttct caggtccggt cctgatggaa atgacagggg aaggcccgcg taacgagcct
     5641 cacctgacgc agcatttcga ttttcggaac ggaaagctgt ggccgaacac caggccggga
     5701 ttgggcgttg aatttgatcc gtcgcgggcc cagttgatcg ctgaaatcac cgagcagcgc
     5761 cgcccgacgc cattgttcca ccgcgcggac gggtcgatca caaactggta actccggcag
     5821 cgtcactttt tgactcacgg cgttccggca gaagagagtc gcccgcctcg caccagaatg
     5881 cggccgccaa cctccaccgt gccatcgggg aagaaaaacc accgcgtgaa gcccccgcgc
     5941 acattgccgc ccagttcctg gttgtcccca agactcagcc gcaccactcc ggcgccatag
     6001 ccgtagtaag cgagcgacgg gctctcggga ggcgcctgta gcttcggatt gaatcccagc
     6061 ccaaattcgc ggaaatgcag ggctgcttcg ccgcccgccg ccagcgcggc gcgcgcagca
     6121 tccaggttct gatccgcctg gatactcgtt atgacgccgt tgcgaatgga aaacctgagc
     6181 ctccgcgcca ctgcgctgcc aaaccgggct gaagggatga ccagagtccc gtttactgtt
     6241 tcttcgagcg gcgctacccg caacacaccg gccggcagct cgatctcgcg atccacccga
     6301 acccgagcgg ctcgggccct agccggcgaa gcatccccgt cctgcttgtt aaacggccgg
     6361 cttcccactc gaaatctcag gtccgttccc gccggcgtgg tcacgtggat cgtaccagat
     6421 cgcagcagcg ctatggcccg atcctgagcg gccgataaag ctgaataatc gatatcgagc
     6481 gccgcttggt aaatctgatc cagggcggga gagtggggcc ccggcaaacc atccgacagc
     6541 acgctgcctt cctgccagtg gaaatgaatt tcgcgccggg cgccgccttt ctccagccag
     6601 cgctgaagag cctcccgctc ctgcggcgaa acttcgtgga gatcggggcg aagaggcagc
     6661 cataggtaaa catcggcatc ttcgattacc tcgccaaagc ctagcggcgg aacccccttg
     6721 gcctgatact ccagcggccc gagcactacc gccccagccc gccggatccg gctctccaga
     6781 gggcctcgaa gctctgcaaa ataatcggaa tcatatcgga gcaagacccg ttctcccttt
     6841 tgcagctcga gagcacgggt gatgcaatcg gccaaatgct catatcgaat atcagccgcc
     6901 cgcccttttg ggccgcccaa agagacggag aggacgatcg ctaggagaac ggaacgtgag
     6961 aaagcgaacg ttaaagggtt catgataaaa tctttaacaa atcctgtcga ggaacaattg
     7021 acaatatagc actaaagttt gataatgtct tttggctaag gtatgctggc ttaacggcca
     7081 cgggcaggag gaatcttatg gctaaaacga ttggcataga tctcggcacc acaaactcgg
     7141 tggtagcggt tatggaagga ggccagccca cggttatcgc caaccaggag ggttcgcgca
     7201 cgacgccttc ggttgtggct tttactaaga gcggcgaacg actggtcggg caagtcgcca
     7261 agcgccaggc gatcacaaat ccggagaata ccattttctc gatcaagcga ttcatgggcc
     7321 gccgttacga cgaagtcaca gaagagatga agatggtgcc ctatcgtgtc gttccggacg
     7381 gcaatctggc gaaagtgcag attggcgaca agaagtactc gccgcccgag atctcggcca
     7441 tgattttgac gaagctgaag gaggctgcgg aatcttacct gggtgaaaag gtcacggagg
     7501 cggtgattac ggtaccggcc tactttaacg attcgcagcg gcaagcgacg aaagacgccg
     7561 gcaaaatcgc cggtctggaa gtaaaacgca ttattaacga gccgactgcg gcggcgctgg
     7621 cttacggtct cgacaagaaa aaagacgaga ccatcgccgt ctacgatttc ggcggcggca
     7681 cattcgacat ctcgattctg gaagtgggtg aaggcgtcgt cgaggtcaag agcacgaatg
     7741 gcgatacgca cctgggcgga gacaacatcg atcatgaggt gatgcagtgg atcatctccg
     7801 agttcaaaaa agagaccggc atcgatctgt ccaaggacaa gatggcgctc cagcggctga
     7861 aagaggcagc ggagaaagcg aagatggacc tctccacgct gcttgaaacc gacatcaacc
     7921 tgcccttcat caccgcggac gcgagcggcc ccaagcacct gcaaatgaag ctgacgcggg
     7981 cgcgtttcga acagatggtc gagccgattt tgcagcgcag cgtcggacct gtgaagcagg
     8041 ctctttccga cgcaggctta acgccggaca agatcgatga ggtcgtcctg gtgggcgggt
     8101 cgacccgtat cccgcgcgtg cagcagatcg tccgcaatct tttcggcaag gagccgcaca
     8161 agggcgtgaa tccggatgag gtagtggcgg tcggagccgc ggtccaggcg ggcgttctcg
     8221 gcggcgaggt aaaagatgtc ctgctgctcg acgtgacccc gctttcgctg ggcatcgaga
     8281 cgctgggcgg cgttttcacc aagctgatcg agcgcaatac gactattcct acgcgcaaaa
     8341 gcgaagtctt ctcgacggct tcggataacc agaccgcagt tgatatcaag gtctaccagg
     8401 gcgaacgtgc gatggctgcc gataaccggc tgctgggcgt gttccagttg gtcggcattc
     8461 ccccggcgcc gcgcggcgtc ccgcagattg aggtgacttt cgacatcgac gccaacggca
     8521 tcctgaatgt ttcggccaag gaccgcgcta cgaacaacga gcagaaaatt accatcacca
     8581 gctcctccgg cctgtcgaag gatgaagtcg aaaaaatggc caaggacgcc gaggcgcacg
     8641 ctgccgaaga ccgcaagcgc cgcgaggaca tcgaagcccg caaccgcgcc gacgcaatga
     8701 tttacaacac cgagaaaacc ttgaaggaac accgggacaa aatcagcgcg gaagacgcgg
     8761 ccaatatcga gagggcgatc gaggaagcga aaaaggcctt gcagcagggc gattcggcga
     8821 ccatcaacgc ggcagtggac aaactgacca cggccagcca caagctcgcc gaagccatgt
     8881 acagggctgc ctcccaggcg ggctcgagtc agcaagcagg tccgcagcaa gcacagcagg
     8941 gccccaaggc ggacggcaag gacaacgtgg tcgatgcgga gttcgtggac gtgaacgatc
     9001 agggtaagaa gtgaagagcg gcgggacagg cttccgcggc ctgtcccgat tcctaagcga
     9061 gtgagtgagc catgccggca acggccaaag aggactacta caagacgctg ggcgtcaaac
     9121 gaaactcatc gcaggaggag attcgcaagg cctatcggcg gttggcgcgg aagcatcacc
     9181 ccgatttgaa ccccggggac aaggcggccg aggaacgctt caagcaggtg caggaagctt
     9241 acgatgtgct gagcgacccc aagaagcgcg aaatgtacga tcaattgggg ttctattcgg
     9301 agactggttt caacgccgga gccggaccgc gaacctcttc cggcccgccg cccgggggct
     9361 tccatttcag cggattcgat ttttccgatt tgttcggatt cggcaatcag gaaggacagg
     9421 catggtcccg gcgcggcccc gaggccggcg gcggattttc cgagatgttc tctcatctgt
     9481 tccgccgggg aggctcgcag gagccggagc cgcagccagg caccgatctc gagtacacgg
     9541 ttcaaatcgg tttttgggac gcgatccggg gcacgcaggc gcgccttaac atttcgcgct
     9601 tcgatgcctg cggccaatgc ggcggagccg gatcgatcgg cggcgcgaag attcagtgcc
     9661 ccgagtgcca ggggacgggc aaagtacggc agatggccgg agccatgcgc ttcgagttga
     9721 cctgcccgcg atgcaacggc aaggggacgc tttccaatac ctgcccggcc tgccatggcg
     9781 aaggacgcat ctcgcggcag gaaacggtcg aagttcgcat tcctcccggc gcggaggacg
     9841 gctcgcgcct gcgcgtagcg ggcaagggca atgccggaac aggcggcgct tcccccggtg
     9901 atttatacat caccacgcgc gtcggcgagc atcccttctt caagcgcaca ggcaacgaca
     9961 ttcatgtcca ggtgccgata accgtttccg aagccgcact gggggcgaag atcgaggtgc
    10021 cgaccatcga cgggcaggcg ttgctcaaga tccccccggg aactgagagc ggaaagaaat
    10081 tccgtctccg ggaaaaaggg gttctgaatc gccgcaccaa cgtccgcggc gatcaatatg
    10141 tggaagtcaa ggttgtcgtc ccgcgcattc aggacgagcg atcgaaagaa atactgcgcg
    10201 aattcgcccg cctgaacccg gaagacccga gggcgggctt gttcgtcaac agatgacggt
    10261 gatgctatgg cacgcaaacg taccagcggc gcttacatga tttccgtcgt ctcggaacag
    10321 tacgacatcc atccgcaaac attgcgcctt tacgagcgcg aggggctgct gaagccttcc
    10381 cggaccgaag gcaacacacg cctttacacg gatcgggatt tggagcggtt agaagtcatc
    10441 ctgtcgctga cgagggatct tggcgtcaat ctcgcggggg ttgagattat tctcaacatg
    10501 cgggaaaaga tggaagcgat gcaacgccag atttctgaat tcgtagcctg cctgagccag
    10561 cagatgaacg gctggcccat cgcccccgaa agtgcgcggc gcgctctggt cccgctttcc
    10621 cagcgatcga cgcccgcgcc gcccggcggc gcttccgctc cggctgccgg ccggcctaag
    10681 tccacatcgc gtcagaagtc gtaacggtag cgccgcgttc ggcccattcg ctgagcagtt
    10741 tttggccggc attctcgtca atcgcgcgga tggcgtctgt aatgacccgc actttccggc
    10801 cctttgccaa caacccctcc acggcgcacc gcacgcaata atcggtggcc acacccacga
    10861 cggcgtactc tcgatccgta gaccggtcca acaagcgctc gaaagtcggg ttcgagaaaa
    10921 tgtccagctt ctgcttctcc acaatgatct gctcgacgcc atccggcagg tcgacgccgg
    10981 gccggttcgg aaccacggca cggtgcttgc ataaagtcgc cgggactttt tgctggccca
    11041 gagttcccgc gacgcagtgc gccggccaga cggcaaactc gggatcgttc tcgctatggg
    11101 catcgaccga cgaaatcacc gggacgcccc gctcccgcgc ttgctcgaag atgcgcgcca
    11161 gcgtgggaat cagatgctcg gcgccaggca cgtaaagcgc cccggccggc agcatgaaat
    11221 cgatctgcgt atcgatgtcg ataagaatca tgttattccg gatcggcgat ctaagccggc
    11281 tcggcccacc cggcctttcg cactgccctg cgtcttactt caactggctg gattccggct
    11341 ggcggcttcc ggcttttccc ctgtctccca ttttcgcact tccctcgtca gctcctgcaa
    11401 cgccgcgctt tggataaccg ggtacttttc cggaaatcgg aagcgccggt atcgctccgg
    11461 cagggcataa aagcgttcca gggcgctctc gcgcagccgc ttgatgtccg gcggatctga
    11521 gattcggcgc ccatcgcgaa ccacctggcg gagcagggcg gcgccgcccg gaaagctctc
    11581 gtctgccagc cctagcacat cgcgatccgg aaaccggaac acctgcttgc agccgggcag
    11641 cgagcgttta tcctcgctga acttggcggt cgggcggacc gacccccggc tctcgatttc
    11701 cactaattta tagatgctgc ccatggcggg agcgtcgcgg gatgtggcca gttccgtgcc
    11761 gactccaaac caatcgatag gagcgcccgc ggccaacagt tcggcgatcc ggtgctcgtt
    11821 caaatcgccg ctaaccatta tctgcgcgcg agagcatccc gcttcgtcca gaatctgccg
    11881 cgctcggcgg ctgtcggcca gcaaatcgcc gctgtccagc cggatgcccc aaaaaggccg
    11941 ctctacgcgg atggcgttct tgatccccgt ctcgacctcg taagtatcga cgagataaat
    12001 tgtgcgttca ccgagcagat cttgcagccg cgagaaggct tcgaactcgg aatcgtaagc
    12061 caacgtccag gagtgggccg ccgtgccgta gacggggatt ccgaagcgaa agcccgcctc
    12121 cacgttcgag gtgcccacgc atccgccgat ataggccgca cgagcgccga gcgttcccgc
    12181 ttcggcggta tgggcgcggc gtgtgccaaa ctcgacgacc ggccgcccgg ccgcggcctg
    12241 gacgatacgc gacgctttcg tcgcgatcaa ggtttgaaaa cttacggccg caagcaggta
    12301 ggtctcggga atttgcgcct cgggaagcgg cgctgtaacc cgcaaaatgg gctcctcggc
    12361 gaacagcggc gtgccttcgt cgaccgccca gacgtctcct gtgaaacgaa acgcgccgag
    12421 atactcccag aaactctccg aaacccgaga gaaaccaggc aaacttcgca aataagagat
    12481 ttccgcaggc gtgaaactga gattcagcag atattcgatc gcttgctgca cgccggccac
    12541 taggatgtag gaacgatcct ccggcaaacg gcggatggag agctcgaaac tcgcgcggtc
    12601 tccggccttc cccgcctcaa aatagccggc ggccatggtc aattcgtaga ggtcggttac
    12661 aagcccgttc acgcgttaac ccttatagca tggcgaggag aggaggctgc ggaatggatt
    12721 gcttacaggc gggccgatgc cagccgaccg cattgggctg tctcacttcc gcagcaggtt
    12781 gctcaccacg aaccagacgt tcgccgggcg ctcggccagg cggcgcatca aatacggata
    12841 ccaggcgtcc ccgaatggaa cgtacacacg catgcgatgc cccgcggcca cgtatcggct
    12901 ctgcaaatcg cggcgaatgc catagagcat ttgaatctca taagagtctt tcgggatgcc
    12961 atgcgacgcc gcgaaatcaa tcgccgcccg cagcagtctt tcgtcatgtg tggccagagc
    13021 gggatcattg ccggcttgaa gaagcatgcg ggccagcttg tcgaagtttg cgtccacgtc
    13081 gcgtttttgg gaaaacgcca ccgacggcgg ctcgtcgtag gctcccttca caagccgcac
    13141 gcgaatcttg cggcggatca attcctcgag atccttttcc gagcgatata aatacgattg
    13201 cacaacggcg ccaatgctcg gaaaatgctc gtgcagatcg gagaccagag caagcgtccg
    13261 gtccgtataa tcgctcgatt ccatatccac gtgaacgaag ctctgcaatt cacacgcccg
    13321 gcgcgccact gccgcgacgt tttcccggca ggcggcttcg gagatgtcga ttccaaactg
    13381 agtcaatttc agggaaacat tggcttgcaa gccctgggcg tgaatgcagt cgagcaattg
    13441 gagatagacc tcgcgggccg cggccgcctc atccaggttg gtgacgctct cgccgaggta
    13501 gtcgagcgtc gtggcaatgc cgccttgatt cagttggcgc gtgacgcgaa gggcatcctc
    13561 aacccgcgtg cccgccacaa accgggatgt cagccggagc gccgcgggcg accgctccat
    13621 ccatcgccgc aatgccgcgc tgcgcgagat gaagagaaaa aagtgccgga gcagcatcag
    13681 aagctacagc agcgatggga gtcgttcctt tggatgcgcc tagacgatcg cttcgcggcg
    13741 gtcgtcccag atcaccgtac gtccttgcag gtagctttcc agcgccatgc ggcacgcaat
    13801 cgacgtgcag aacccgatct cgaacggaca gttggggtct ttgccggagc ggatgcaatc
    13861 gagaaagttc tgcatgtgcg ttccgccgcg gttcgatccc agcatttcgg aggtgcgagg
    13921 ctgattcact ttctccggga tgtagcgtac ctgccggctt cgcacgatcg ttccgtcgct
    13981 tcccagcgcc tcttcgcttg agcccaggtg gccgttggcc agtccgctcg accaggtgaa
    14041 cagcatctct tccggctgtt ccatgatgac gtgcagcgtg tcgggcgcct cgcggccgtc
    14101 ccgccaatgg tagatgccgc cccgggtggt gaccgccctc ggaatgcgta aatccagggc
    14161 tttgtaccag aacgccgcct ggtggctcat attctcgtaa atgctgccgc cggaataatc
    14221 aaaaaagagg cgccagttga taaaccggtt cgggtcgaat tcgcgctgcg gcgcctcgcc
    14281 aaggaacatg ctccagtgca cattctccgg cttcatatcc gggtagacag gccgcgccca
    14341 atgcggccgg ccgtgaggcg tattgcgata ggcccaggcg tgaatttcgg tgatgcggcc
    14401 gaccaagcct gtggccagga tctgccgaac atcctcaact tgtccggaac tgcaagcctg
    14461 atggccgatt tgaaggaccc ggccggcccg ctggaacgcc agccgcacgc gcttggcctg
    14521 gtccactgtg aacgccatcg tgttttccag ataaacatgc cggccggctt cgagcgcggc
    14581 cacgcagtgt tcggcgtgca aatgctgggg agtggcgatc aagaccgcgt cgatcgatgg
    14641 atcctccaac atttcgcggt gatcgaagtg cgcccgggcc gagggcgcga tgagccggca
    14701 ttcctcgtga cgccggcggt atacatcggc cacagcaaca agctccgtat tggggcaggc
    14761 caaagcttcg cgcaggatct gctgcccgcg cgcaccggcg ccgataagcc ccagccgtat
    14821 ccggtcattg gcgccgagga ttcgagcggc cggcgtcatc gtggcgattg ttccggccac
    14881 cccaaccgaa accttgccga taaagttgcg ccgcgaggtc atagagccat tctgattcta
    14941 gcagacccgc ctccttcgcc cctgggaagc agcgaatgcc agccgtagga ggaaccggca
    15001 ggctcctcga agcgcggccg cgccggccgc cgaaggcagc gcgcgaggcg gtttgcgagg
    15061 cgagaagttc aggcttcggg ggccatgagt ttctcggctc catcgccggt tccttgcgcg
    15121 accaggctgc tgtacactaa gcgacacaa
//
LOCUS       DASREK010000292         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2442786, whole genome shotgun sequence.
ACCESSION   DASREK010000292 DASREK010000000
VERSION     DASREK010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442786"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VFA54_15805"
     CDS             <1..1785
                     /locus_tag="VFA54_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZS52334.1"
                     /translation="KILAGLEPLDAGGIDRARGLSVGYLPQDGLALAGRTVFEECLSV
                     FAEVLALEREQRELAEKLGRLDPAGEEYRAAAERFHRVQDEFQALDGYTAEARVGSVL
                     AGLGFPKQQWRRRTEEFSGGWQMRIALAKLLLEKPNLLLLDEPTNHLDLEARNWLEDY
                     LNGYPHAFVIVSHDRYFLDVTVKKIAELWNRGVHFYAGNYSRYETLKQNRLAQLEAAY
                     KNQQERIHHLEVFINRFRYQATKASQVQSRIKELEKIERIELPAEEKTIHFRFPQPKA
                     SGRVVQEFRHVAKSYGPNRVFDDLNFLIERGDRIALVGPNGAGKSTLIRMLAGAEPLT
                     AGERIVGHNVGIDYFAQDQYKALDPQKRLLDDLSAVVPRATENELRSLLGCFLFSAGD
                     VFKPIGVLSGGERNRYALARMLLVPSNFLLLDEPTNHLDLRAKDVLLEALLEFSGTVA
                     FVSHDRYFIDKLATKVFEVGNGAVNIYSGNYEDYLWRKASEDGQGSGEDGKRPAAQKE
                     AAGKSARTASRVNPLKAEEMRRRIAEIEQQTAGIEREIGLLEAQLAGSYRDHRESMRL
                     TKEIEQRRARLDALISEWEQAGTALEQA"
     gene            complement(2730..>3149)
                     /locus_tag="VFA54_15810"
     CDS             complement(2730..>3149)
                     /locus_tag="VFA54_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52335.1"
                     /translation="YGGDGITPDVQLPRHTADRFQTELFRRFAFFNYATHYFAAHESL
                     PANWDTDEAALEDFRQFLTSEKISFTSAEFARNQDFIRRFLKREMYIHAYDLDAGERV
                     KAERDPDVQAALDLLPKAQQLTNQAQRMLAQRTGRKP"
BASE COUNT          612 a          921 c         1002 g          614 t
ORIGIN      
        1 aaaatcctcg ccgggctgga gccgctcgat gcgggcggga tcgatcgggc caggggcctg
       61 agcgtaggtt atctgcccca ggacggcctg gccctggcgg gcagaaccgt ctttgaggaa
      121 tgtctgagcg tttttgcgga agtgctggcg ctcgagcgcg agcagcgcga attggctgaa
      181 aagctgggcc gtctggaccc ggcgggcgaa gaatatcgcg ccgccgccga gcggtttcac
      241 cgcgtgcagg acgagtttca agcgctcgac gggtacacgg ccgaggcccg ggttggatcg
      301 gtgctcgccg gcctcggttt cccgaaacag cagtggcggc gccgaaccga ggaattctcg
      361 ggcggctggc agatgcgcat cgccctggcc aagctgctgc tcgagaaacc gaacttgctg
      421 ttgctcgacg agcccacaaa ccatttggat cttgaggcgc gcaactggtt ggaagattat
      481 ttgaacgggt atccgcatgc gttcgtcatc gtttcgcatg accggtactt tttggacgtc
      541 acagtaaaga aaatcgccga gctctggaac cggggcgtgc acttttatgc aggcaattat
      601 tcccgctacg aaacgttgaa gcagaaccgg ctcgcccaac tggaagctgc ttacaaaaac
      661 cagcaggaaa gaattcatca tctggaagtc tttatcaacc gtttccgcta ccaggccacc
      721 aaggccagcc aggtgcagag ccgcatcaaa gagctcgaaa agattgagcg catcgagctg
      781 ccggcggagg agaagaccat tcatttccgc ttcccgcagc caaaggccag cgggcgcgtc
      841 gtgcaggagt tccggcacgt tgcgaagagc tacggcccca accgcgtttt cgacgacctg
      901 aattttctca ttgagcgcgg cgaccgcatc gctctggtgg gacctaacgg agcgggcaag
      961 tccacgctga ttcggatgct ggccggggcc gagcccctca ccgcgggcga gcggatcgtg
     1021 ggacacaatg tcgggatcga ttatttcgcg caggatcaat acaaagcgct cgatccgcag
     1081 aagcgccttc tggacgacct ctccgcggtg gtcccgcgag ctactgaaaa cgaactgcgg
     1141 agcctgctcg gctgttttct gttttcggcg ggcgatgttt tcaagccgat tggcgtgtta
     1201 tcgggcggcg agcgcaaccg ctatgcgctg gcccgcatgc tgctcgtccc ctccaacttc
     1261 ctgcttctcg atgaaccgac caatcattta gacctgcggg ccaaggacgt gctgctcgag
     1321 gccctgctcg aattttcggg tacggtggct ttcgtctccc acgaccgcta ttttatcgac
     1381 aagcttgcca cgaaggtatt tgaagtaggc aacggagcgg tgaacatcta ttccggcaat
     1441 tacgaagatt atctctggcg caaggcgagc gaggacgggc aggggtccgg agaagacggg
     1501 aaaaggcccg ctgcgcaaaa ggaggccgcc ggaaaatccg cgcggacagc gagccgcgta
     1561 aaccccctga aggcggaaga aatgcggcgg cggatcgctg agatcgagca gcagacggcg
     1621 ggaatcgagc gcgaaatcgg gctgctcgaa gcccaactgg cggggtccta ccgcgatcac
     1681 agggaatcga tgcgcctgac caaggaaatc gagcagcgcc gcgccaggct ggacgccctg
     1741 ataagcgaat gggaacaagc gggaactgcg ctggaacaag cctgagcccg cgtgcgctcc
     1801 ggactcgctg gatcccacga ctttctctcg accaccgtaa tgccgcgctc tgcgccgctc
     1861 tgtccgtggc ttccgtgctg gccacgatcc cgtttttgga gacaggcttc aacgacgact
     1921 ggtctttaca cctttacggc gctacgactg gccgagacgg gaaagctgac gtataagctg
     1981 gggcgcgctg atacttggcg tgcaggcgtg ctcggccgcc ctgatcatcc ggcttcttgg
     2041 gtttggtttc actgcggtcc ggctctccac gctggtgttc gacgccggct cggcagccgt
     2101 tcgccgtgcc ggagggcccg ctctggcgcg ttccacaagc ttccctgcca ttcagcgctg
     2161 tttgggacac gttgttccgg atgggtgtca gccttgccct cctgattctt cccgttctgg
     2221 ccgtctacat accggggggt tgtggatctc cgatcgctgg ctcttgctgg cgctgttctg
     2281 ataatccctt gggctttgtg gaaagcaacg gcgcccggtt cgccgggcta tagtccaatg
     2341 ctcggcaata tcattacccc gcagggaatt ttggatggcc tggatgccat agggaacaag
     2401 cccacgacgc tgactcggcc gctgcaaatc tcgcctcagg cggcgtcccg cggcccccga
     2461 atcaggagaa aggcctgttc acgaggcgcc gaaaataaca gccgatcgtt ccggcaaggc
     2521 ccaggcccgg ctcgaaagcc cggcttggcg tggaatcgcc gccaagatgc agcgaggcgg
     2581 tggagtcagg atgcatggac ccaggtgcgg gaacttaagc catcaatcgc cgcgcggtga
     2641 gaacttgtgc cgccgggccg tgaaacttga catccgagag atgcagcgcg gagatgtatc
     2701 cgcgtcccgg gcgaggctcc ggcggcgtgt cacggtttgc ggccggtgcg ctgcgccagc
     2761 attctctgcg cctgattggt gagttgctgg gctttgggca gcagatcgag agcagcctga
     2821 acatccgggt cgcgctcggc ttttactctc tcgccggcgt cgaggtcgta ggcatgaatg
     2881 tacatttcgc gcttgaggaa ccggcggata aagtcctggt tccgtgcgaa ctcagcgctg
     2941 gtaaaagaga ttttctccga cgtcaaaaac tgccggaagt cctccagcgc tgcttcgtcc
     3001 gtatcccagt ttgccggcag gctttcgtgt gcggcgaagt aatgcgtcgc gtaattaaaa
     3061 aacgcaaagc gccggaaaag ctcggtctgg aaacggtctg cggtatggcg gggaagctga
     3121 acatctggcg tgatgccgtc gccgccgta
//
LOCUS       DASREK010000293         4718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_810555, whole genome shotgun sequence.
ACCESSION   DASREK010000293 DASREK010000000
VERSION     DASREK010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810555"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFA54_15815"
     CDS             <1..759
                     /locus_tag="VFA54_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52336.1"
                     /translation="FPNVQSIALAGNWGTGNYHSLQTGVEHRFRSGLNLVSNYTWSHL
                     IDDYPVVGGGKVGQGPWPQLVNNFHLERGNSDIDIRHRWTLMANYQLPFGRSLTGWRG
                     VVAKGWQANGIAVLQSGPTMTVQNGSPRANTGSGDRPNQIADGNLSADQRSTARWFNT
                     AAFAPQPLFQIGNAGRNTLFAPGRGELDFSVFKEFKPTERTTLQFRAEAFNITNTPNF
                     GVPGNQLGTAAFGVISDTANTLPRNLQFALKLLF"
     gene            complement(844..1494)
                     /locus_tag="VFA54_15820"
     CDS             complement(844..1494)
                     /locus_tag="VFA54_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52337.1"
                     /translation="MPESTPAVQQHQLPPLILHPFTDASSSVRALEDAKSAAQQLHLD
                     GGALSRAEQLRKRLLEGRYAELRMLFFVGKDVFRWLGQCMDYTQRTESLRSRGLAEQS
                     FAEFLISDTPDEVAAKLRRWGVLDYARIFARSIGIQAQFREPPPREILSAEYLRDYYR
                     FADYSYTCWKESVKFPLLAAGDFSFCLYASGEYAKMLEQQWSAAENGDGRNPDAAR"
     gene            complement(1598..3412)
                     /locus_tag="VFA54_15825"
     CDS             complement(1598..3412)
                     /locus_tag="VFA54_15825"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS52338.1"
                     /translation="MRLARNLVFLLGASALCWAAGADVQRYIRDVRTLAAPEMKGRGD
                     GSPELEKAAHYIADQFRAAGVEPLNGDSYFQPFTISVGANLGPDNRFEIESGGRTTAL
                     RVGQDFTPLSFSSSGSVSGTMVFAGYGITAPEYHYDDYAHLDVKDKIVLVLRHEPQEH
                     DPKSVFRGRAFTRHAAFISKAINARNRGARGLIIVNDRGAHASDEDRLVKFGFTEGPP
                     DEGLPVLQVKAAIAEQWVAEAGKSLAKLQKEIDKTLEPQSFALPESIRVSARVNIERQ
                     MATVNNVVAWLPGSSDEYIVIGAHYDHLGLGQYDSLAPSMVGKVHPGADDNASGAAGL
                     MELARMFAARKSELKRGVVFLAFSGEELGLLGSNYYVNHPLKPLDKTVAMINLDMIGR
                     VRQDKVYVGGVGTGTTFRPMVEEAGKKFSFHLDYSAGGYDSSDHTSFNAKQVPVLFFF
                     SGLHSDYHKPSDTWDKIEGPETVRLLDMVFDVAENLDEAGSRPQYVRVAESNPHAGSA
                     AGDSGGGGYGPYFGSIPDFGQVENGVKFADVKEGSPAAKAGLKSGDVLVEFDGKPIKN
                     LYDFTYALRSKNVGDRVEVRVLRDGKPLTARVTLEQRR"
     gene            complement(3425..4435)
                     /locus_tag="VFA54_15830"
     CDS             complement(3425..4435)
                     /locus_tag="VFA54_15830"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZS52339.1"
                     /translation="MLHNFRSVGLCLVGAGALLAAPPQAARKAASGKTASTARAATKS
                     AAAASSDPKSALNKQHLEAYVRHLYGWLPEVKVQISDFTPSPVPGLLETTVHATYELA
                     SQDKVFYVSADGKHIIDGAVYPADDNPFRDNLSKITTALQPSFGPAGAEVVIVAYSDF
                     QCPHCREEAKSLRENITKTFPTQVRVYFKDFPLPNHDWAKTAAIAGRCIYRQNPQVFW
                     QYHDWIFENQSEILTRNFSEKLNEFIQGKRVDPLQLNQCLEKRDTEAEVDKSIAEGKA
                     LGINSTPTVFVNGRKLSGATPWPTLQRVIELELDYQKAAKQSDEACCEVKLPTPFTQP
                     QH"
     gene            4525..>4718
                     /locus_tag="VFA54_15835"
     CDS             4525..>4718
                     /locus_tag="VFA54_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52340.1"
                     /translation="MTAPRPVRYAQSRSSGILARYIFKEIVSSALLGTVLFTFILFLR
                     KVPQLFELLVRRTASGEVVL"
BASE COUNT          925 a         1434 c         1391 g          968 t
ORIGIN      
        1 ttcccgaacg tgcagtccat agctttggcc ggcaattggg gcacgggcaa ctaccactct
       61 ctgcaaaccg gcgtggagca tcgcttccgc agcgggctga acttggtgag taattacacc
      121 tggtcgcatt tgatcgacga ctatccggta gtgggcggcg ggaaagtggg gcaggggcct
      181 tggccgcagc tggtcaacaa tttccatctc gagcggggaa attccgacat cgatatccgc
      241 catcggtgga cgctgatggc caactatcaa ttgcccttcg gcaggagcct caccggctgg
      301 cgcggcgtag ttgccaaagg gtggcaggcg aacggaattg cagtgctcca aagcggtcct
      361 acgatgaccg tgcagaacgg atcgccgcgc gccaataccg gctccggcga ccgcccgaac
      421 cagatcgccg atgggaacct ctccgccgat cagcgcagca ccgctcggtg gttcaacacc
      481 gccgcgtttg cgccccagcc gctgtttcaa atcgggaatg cgggacgaaa cacgttgttt
      541 gctccgggac gcggcgagct tgatttttcc gtgttcaagg agttcaagcc aaccgagcgc
      601 acaacgctgc aattccgggc cgaggcgttt aacattacga acacgccgaa cttcggtgtg
      661 cccggcaacc agcttggaac cgccgccttc ggcgtgatca gcgacaccgc aaacacgctg
      721 ccgcgtaact tgcaatttgc cttgaagctg ctgttctaaa tatcagactg ctttgtcacg
      781 cgttcaggtt gaacgcggcc aagttcttct ttgaacgtaa ctgagcccgg ccggacgagg
      841 gggttatctg gccgcgtccg gatttcggcc atctccgttc tctgcggcgc tccactgctg
      901 ttccagcatt ttggcgtact cgcccgaagc gtagaggcag aagctgaaat cgccggcggc
      961 gagcagggga aatttgaccg actctttcca gcaggtatag gaataatcag caaagcggta
     1021 gtaatcgcgg aggtactcgg ccgacaggat ctcccggggc ggcggctcgc ggaactgcgc
     1081 ctgaatgcct atggaccgcg cgaaaatgcg ggcgtaatca agcacgcccc accgccgcag
     1141 tttcgcggcg acctcgtccg gggtgtcgga aatcagaaac tcggcgaagc tctgttccgc
     1201 cagcccccgg cttcgcaagg attctgtgcg ctgcgtataa tccatgcact gccccagcca
     1261 tcgaaaaaca tctttgccca caaaaaacag catgcgcagc tcggcgtagc ggccctcgag
     1321 cagccgcttg cggagctgct cggcccggct caaggcgccg ccgtcaagat gcaattgctg
     1381 agcggcggat tttgcgtctt caagagcccg caccgaactg gaggcatccg taaacgggtg
     1441 aagtatcagc ggcggaagct ggtgctgctg gaccgcaggc gttgattcgg gcatcggctc
     1501 ttcttttctc atcggcccgc gcccccgaaa ataacgcggc tggtaaaaac ttccgctgcg
     1561 ccgaacgcag cttgtaaaat cagccgcaac ttagaagcta tctccgctgc tcgagcgtga
     1621 cccgcgccgt cagcggcttg ccatcgcgca agacacgcac ttccacgcgg tctccgacat
     1681 tcttgctgcg cagggcgtag gtgaagtcat aaagattctt aatcggcttg ccgtcaaact
     1741 ccaccagaac atcccccgat ttcaggccgg ccttggccgc cggcgagccc tctttgacgt
     1801 cggcgaattt cacgccgttc tctacctgcc caaaatcggg aatggagccg aaataggggc
     1861 catagcctcc gccccccgaa tctcccgccg ccgagccggc gtgcggattg ctttcagcga
     1921 cccggacata ctgcggccgg gagcccgctt cgtccaagtt ctcggcgacg tcgaagacca
     1981 tatcgagcag ccggaccgtt tccggacctt caattttgtc ccaggtatcg gaaggtttgt
     2041 ggtaatcgct atgcaaaccg gagaagaaaa aaagcaccgg aacctgcttg gcgttgaaac
     2101 tggtgtgatc gctcgaatcg tatccgcccg cggagtagtc caaatggaac gaaaacttct
     2161 tccccgcctc ctccaccatc ggacggaatg tcgttccggt tccgacgcct ccgacgtaca
     2221 ctttgtcctg gcggacgcgg ccgatcatgt cgagattgat catggccacg gtcttatcga
     2281 gcggtttcag gggatgattt acgtaataat tggacccgag caagcccagt tcttcgccgc
     2341 tgaaggcaag aaagaccacg ccgcgcttca gttccgattt gcgggcggcg aacatccggg
     2401 ccagttccat cagccccgcc gcgcccgaag cattgtcatc cgcgcccgga tggactttgc
     2461 ccaccatcga cggagccagc gaatcatatt ggcccagccc gagatgatca taatgggcgc
     2521 cgatcacgat atattcatcc gacgaaccgg gcagccaggc gacgacatta ttgacggtag
     2581 ccatctgccg ctcgatgttg acgcgagccg atacgcgaat ggattccggg agcgcgaaag
     2641 attgcggctc gagcgtttta tcaatctcct tctgcaactt ggccagcgat ttccccgcct
     2701 cagccaccca ttgctcggcg atcgctgctt ttacctgcaa cacaggcagg ccctcgtccg
     2761 gcggaccttc cgtgaacccg aatttcacca gccggtcttc gtccgaggca tgagcgccgc
     2821 ggtcgtttac gatgatcagc ccgcgggcgc cccggtttct cgcgttaatg gccttgctga
     2881 tgaaggcggc gtggcgggtg aacgcccggc cccgaaacac gcttttggga tcgtgctcct
     2941 ggggctcatg gcgcagcacg agcacgattt tgtccttcac gtccagatga gcgtaatcgt
     3001 cgtaatgata ctcaggggcg gtgattccat agccagcgaa caccatcgtt cccgaaaccg
     3061 agccgctgga ggagaaactc agaggcgtaa agtcctggcc gacgcgcagg gccgttgtac
     3121 gtccgcccga ctctatctcg aagcggttgt ccgggccgag attggctccc accgagatcg
     3181 tgaaaggctg gaaatacgag tcgccgttga gcggctcgac gcccgccgcc cgaaactggt
     3241 ccgcgatgta atgcgcggcc ttctccagct cgggggaacc atcgccgcgg cctttcatct
     3301 cgggcgcggc cagcgtacgg acgtcgcgga tgtatcgctg gacgtcggcg cccgccgccc
     3361 agcacagagc cgatgctccc agcaggaaaa cgagattgcg cgccagccgc atggtgaacc
     3421 cccgctagtg ttggggctga gtgaatggag tcgggagctt gacctcgcag caggcttcgt
     3481 cgctctgctt ggccgccttc tgataatcga gctccaattc gatcactcgc tgcaaggtgg
     3541 gccagggcgt agcgcccgac agtttccggc cgttgacaaa caccgtcggc gtcgagttga
     3601 ttcccagcgc tttcccttcc gcgatggatt tatcgacttc ggcctctgtg tcgcgcttct
     3661 ccaggcattg attcaactgc aagggatcga cccgtttccc ttggataaac tcgttcagtt
     3721 tctcgctgaa gttccgggtg aggatctcgc tttggttttc gaaaatccaa tcgtggtact
     3781 gccaaaatac ctggggattc tgccggtaga tgcagcggcc ggcgatggcc gccgttttgg
     3841 cccagtcatg attcggcagg gggaaatcct tgaaatatac gcggacctgc gtcgggaagg
     3901 tcttcgtaat attctcgcga aggcttttag cctcctcgcg gcagtgcggg cactggaaat
     3961 cgctgtaagc tacgatcacc acttccgcgc cggccgggcc gaagctgggc tgcaaggccg
     4021 tggtaatctt gctcaggttg tcccgaaacg ggttgtcgtc cgccggatac acggcgccat
     4081 ctatgatgtg cttcccgtcc gcgctcacat aaaaaacctt gtcctgcgag gccaattcgt
     4141 aagtcgcgtg aaccgtggtt tcgagcagcc ctgggaccgg ggaaggggtg aagtcggaga
     4201 tctgtacttt gacctccgga agccacccgt acaagtggcg gacgtaagct tccaggtgct
     4261 gcttgttcaa tgcggatttc ggatcggagg aagcagcggc ggcgctttta gtcgccgccc
     4321 gcgctgtgga agctgtcttg ccagaggctg ccttcctggc tgcctgggga ggggccgcca
     4381 gaagcgctcc agcgccgacg agacaaagcc caaccgagcg gaagttgtgc agcatagaac
     4441 caattgtatc aattttcgcg ctgccgggcc gccgcacgcg atttgagcgg cgtgcggcgc
     4501 gaatgttaag atcgctagcg ggaaatgacc gctccgcgac ccgtcaggta cgcccagtct
     4561 cgcagttccg gaatcctagc acgttacatt ttcaaggaaa ttgtttccag tgcgctgctg
     4621 ggaacagttc tgttcacttt catccttttc ttgcggaagg tgccgcagct ttttgagctg
     4681 ctggttcgcc ggacggcttc gggggaagtg gttctcta
//
LOCUS       DASREK010000294         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2121090, whole genome shotgun sequence.
ACCESSION   DASREK010000294 DASREK010000000
VERSION     DASREK010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121090"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..671
                     /locus_tag="VFA54_15840"
     CDS             96..671
                     /locus_tag="VFA54_15840"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS52341.1"
                     /translation="MLRLSRFSKLAAAAAILLTGLAWAGEVPRPAPPLTVKTLRGEPL
                     SLESLRGKVVLLEFFLTTCPHCQRTAGTIEPIWREYRSRGLEVLAVAINPDAAQLIPD
                     FRARFGSTYPMALGSSDQVKAFAGISPVKTFFVPYIFLIDRKGVIRYEHPGEDAAFYQ
                     NENTNMRAEVDALVKEGAPARKSTAKAAKKG"
     gene            848..2200
                     /locus_tag="VFA54_15845"
     CDS             848..2200
                     /locus_tag="VFA54_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HZS52342.1"
                     /translation="MSGPSLVVLTLLSIVLLLALVLVLRLHAFLALLISGMALGAAAG
                     LPLRKVLSSLQFGFGDALGFIAVVVGLGAMIGRFLEVSGGGQVLADWLLQLFGRERAP
                     WAVLATSFLLGLPIFFEVGFIMLAPIIWNLGRETKRSLLFYGLPMAAALTTVHAMVPP
                     HPAPSGAAQLLGADLGRTILYGVAFSVPMAISGGMIYGGWIAQRIFVPVPEHMSRPAE
                     LVADNHEELKPPRVGLVLTALLIPVGFIFAASISDMVAPPASRVRQALGFFGHPFTAL
                     MTATLFVMVFLGARRGAKRDQIAKLATECLAPTASLLLIIGGGGAFKQVIVDSGVGKY
                     AGELLAASHISPLVVAYIIAACLRVAQGSATVAIVTAAGILAPIVKGIPGYRPEMLVM
                     ATSAGGTILSHVNDAGFWIVKEYFGMTVPDTLRTWSVMKVVLSLAGFGCILLAQAVFF
                     "
BASE COUNT          343 a          668 c          694 g          522 t
ORIGIN      
        1 accgctgttc tcaagggaag ccagcggcgt cccgcctgcc aacgcctggc ccgcttgcca
       61 tgcgcctcgg ctccggacta ggatacatta ttgttatgtt gcgtcttagt cgattttcca
      121 aattggcggc tgcggcggca atcctgttga ccgggttagc ttgggcaggc gaggtcccga
      181 ggccggcgcc acccctgact gtgaaaactt tgcgcgggga gccgctctcg ctggaaagtt
      241 tgcggggcaa ggttgttctt ctggagttct ttctgacaac gtgcccgcat tgccagcgta
      301 cggcgggaac gatcgaaccg atttggcgtg aatatcgctc gcgcggtctg gaggtcctgg
      361 cagtggcaat taatccggat gccgctcagc tcattccgga cttccgggcg cgctttggct
      421 ctacgtatcc gatggccctc ggcagcagcg atcaagtaaa agcgtttgcc ggcatctcgc
      481 ctgtgaaaac cttttttgtg ccctatattt ttctcatcga ccgcaagggc gtgatccgct
      541 atgagcaccc cggcgaagat gctgcctttt accagaacga gaacacgaat atgcgcgccg
      601 aggtggacgc gctggtgaag gaaggggcgc ccgcccggaa atcgaccgcc aaagctgcaa
      661 agaagggctg aaacggcgag tgaaagcccg gcttgggatg atcccagccg caccgagcag
      721 cgccttcttt tcactttcac tgacaacatc tcgcatctcc tgaggcgttc ccgcccgggg
      781 ccctggacat cagttccgcc catctcgtat atttgccgag atctccgacg cggaaaggtt
      841 gacacatatg agcgggccgt ctctggtggt tctcacactg ctgtccatcg tcctgctttt
      901 agcattggtg ctggtgttgc ggttacacgc gtttctggcc ctgctgattt cggggatggc
      961 gctgggagcg gctgcggggc tgccgctgcg caaggtgctg tcttcgcttc aatttggctt
     1021 tggcgacgcg ctggggttta tcgccgtagt ggtcgggctg ggcgcgatga tcggccgttt
     1081 tctcgaagtt tcgggcggcg gccaggttct cgcggactgg ctgctccaac tgttcggccg
     1141 tgagcgggcc ccttgggccg tgctggccac gtcgtttctg ctcggtcttc cgattttctt
     1201 tgaagtcggc ttcatcatgc tcgcgcccat catctggaac cttggccggg aaaccaaacg
     1261 atcattgctt ttttacggcc tgccgatggc tgcggcgttg accaccgtac acgccatggt
     1321 tcctccgcac ccggccccct cgggagcggc tcaactcctg ggcgcggatc tcgggcgtac
     1381 gatcctctat ggggtcgcct tcagcgtccc gatggcgatc tcgggcggca tgatttacgg
     1441 cggctggatc gcccaacgca tctttgtgcc cgtgcccgag cacatgagca ggcccgccga
     1501 gcttgtcgcg gacaatcacg aagaacttaa gccgcctcgg gtgggcctgg tattgactgc
     1561 gcttctgatt ccggtgggct tcatcttcgc ggcttccatc agcgacatgg tcgccccgcc
     1621 tgcctcgcgc gtacggcaag cgcttggatt tttcggccat cctttcacgg ctctgatgac
     1681 cgcaacgctg tttgtgatgg tgtttcttgg ggcccgccgc ggcgccaaac gcgaccagat
     1741 tgcgaagctg gctaccgaat gcctggctcc caccgcctcg ctgctgctga tcatcggcgg
     1801 cggcggcgcc ttcaagcagg tcatcgtgga ttcaggcgtc ggcaagtatg cgggcgagct
     1861 gctcgccgct tcgcatattt cgccgctggt agtggcttac attatcgcgg cgtgcctgcg
     1921 cgtcgctcaa gggtcggcga cggtcgccat cgtcaccgcg gccggcattc tggctccgat
     1981 tgtgaaagga attcccggct accggcccga gatgctggtg atggccactt ccgcgggcgg
     2041 aaccatcctg tcgcacgtca acgacgccgg tttttggatc gtcaaggaat atttcggtat
     2101 gaccgtgccg gataccttgc gcacctggag cgtgatgaag gtcgtcctgt cgctggcggg
     2161 gttcgggtgc attttgctgg cgcaggccgt gtttttttag agagccggcg tcttggagtt
     2221 tggacct
//
LOCUS       DASREK010000295        29377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_481517, whole genome shotgun sequence.
ACCESSION   DASREK010000295 DASREK010000000
VERSION     DASREK010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 29377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29377
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481517"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFA54_15850"
     CDS             <1..464
                     /locus_tag="VFA54_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52343.1"
                     /translation="AKLRAFGAAAGWILPEDAAPPVNRGEALIWSKNEKPWRARIIPG
                     RAERLRHARKYAAGEIGPGMEFCFRGPEGKLNLRATNLITFVQLAAGVDPETWSYHLR
                     RGEYSRWFRDAIRSEELALEAERIEKDRKLSPAESLARIRRAIESRFTLPG"
     gene            543..1961
                     /locus_tag="VFA54_15855"
     CDS             543..1961
                     /locus_tag="VFA54_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZS52344.1"
                     /translation="MANPDVIVIGSGPNGLAAAITLAQAGRSVVVFEAEHTPGGGARS
                     AELTLPGFVHDVCSAVHPLALASPFFRTLPLAEHGLAWIEPPAALAHPFDDGTAALLE
                     RPVQATAQHLGEDQSAYIKWMTPLVEDWARLEASILGPPRWPRHPLTLARFGASALRP
                     ASALARSLFRGRRARALFSGCAAHAMMPLEKPLTAGFGMVLALLAHVVGWPLARGGTQ
                     SLTDALISYLRSLGGEVLTGARVTSIDELPPSRAILCDLSPKPLLQIAGHRLPGAYRR
                     KLERYRYGLAAYKIDWALDGPVPWRAAECTRAATVHLGGTMEEIAASERECWSGRPPE
                     RPFVLLVQPSLFDPARAPAGKHTAWAYCHVPNGCSFEMASRIEQQMERFAPGFRDRIL
                     ARHIMPPAEIEKHNPNLVGGDIASGVPDLRQFFARPAWSLYSTPINGLYICSASTPPG
                     TGVHGMCGYFAARRALKNLWGG"
     gene            2201..4015
                     /locus_tag="VFA54_15860"
     CDS             2201..4015
                     /locus_tag="VFA54_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS52345.1"
                     /translation="MLVDIPAPASASAERDERLREGKGGLASPVWQELLKPYRGAYSK
                     YIAGVILRQAILVLGGYSMVWAIRLCTGRPTFPEWLVIAALLLFDSVYLSLDTGLNTL
                     FTTRLSFPLFGRMKIAALEKIFQMPTEWHQEQSSGTLLGKVNNGVGRVMGMGEAVSRE
                     LLPAAIRTILSLVPLLLLSLYTAPLLLLALGLFSWLTLRENNRRAALRSKRHACYVRD
                     SGKFGEYVQAAQPVVQFGQTRRLLADYGRLQERIVRLGRAEMRVANTYSWRRSLVLSL
                     AKRVSQGIWIWQLRRGALDVAMAMYLNLLTEELLASFWGYAGLLERIFEGLEPARILV
                     KLLNEQPAVRDQPGPAPAPAEGDCGIEMIDVQFSYSRGKHIVRNLNLSIKRGEVLGIV
                     GRSGSGKTTIQNLLSRIFEAQHGKITVYGADIRQWPLGRLRGLFACVSQNGGVFFSGM
                     TLFDTIRFARPEATFREVVEAAKCACIHQDIIRMPAKYRTKMKAGGANLSKGQQQRIA
                     LAQALLALKDDRKILVLDEFTSQLDSETESKILANLLPHLAGRTVILIAHRLSTLRQI
                     ADRIAVLEDGRIIEQGAHEELVRSNGWYAEMARAQAVT"
     gene            complement(4008..5309)
                     /locus_tag="VFA54_15865"
     CDS             complement(4008..5309)
                     /locus_tag="VFA54_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04295 family B12-binding domain-containing
                     radical SAM protein"
                     /protein_id="HZS52346.1"
                     /translation="MRFLLLNPNWSFEGSIYFGCRYPHLPLEYGYAQALLRQRHHEAV
                     IIDAQLENLSLEQVRAAAAAYRPDFLVVTTAPSYLFWRCAPPELRVPLEALQAVRDLA
                     GKIAVVGPHGSTTPRAVLRKLEPDAVVLGECEEILPKLAGDWKEIPSLCYLDHGEPVI
                     QGGPHACDMARLPALDWPQEAIRLHRHQHHRFDAAPSGPGAEMETTRGCPYHCTFCAK
                     DNFRNSFRRRPLCAVLVELDGLLAKGVEYIYFIDEIFLPYADLLEALARRKIRFGIQT
                     RIDLWAPPMIDLLGRAGCVSIEAGVESITEDGRALLDKRCKLSTADLAERLIYARQRV
                     PFVQANLLESQNDNPAEVEAWRAELRQHGVWANRPVPLFPYPGSPEYLRRWGAPDDVA
                     WERAYREYLAAFSEFSDIQEERPLALEQLERSAAGPQSWTK"
     gene            complement(5290..7356)
                     /locus_tag="VFA54_15870"
     CDS             complement(5290..7356)
                     /locus_tag="VFA54_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS52347.1"
                     /translation="MKRPPGQRARNHEETPRKAGPACGGQRVGMVECFFPGEYERVEA
                     TLADLKSLGVEDLRTTICWADWYRSEGDGWYAWLLPRLAKEVSILPCFLYTPPSLGIV
                     PKISSPPRTPKAYADFLDVMITRFGRLFEWIEFWTEPNNIDYWDYRLDPGWEIFCEMI
                     GGAAYWARKRGKKTVLPGAWPPDCRWLKLMFDRGVMAYVDAVGIHGFPGVSEHPWSGW
                     TKHIQDTQSALAEGGSAAQIWITEAGYSTWREDERAQLRAFAEAVEAPVPRVYWKALQ
                     DRAAEGGALRTDERDCHFGLKQGDGKSKLLFQVWARGGIEEVRDKARADQRARSTGRR
                     RHVLITGGAGFLGANLAHRLLSGGQSVLLFDNLSRPGTERNLHWLQERYGDRVHLEAA
                     DVRDAAALRAAVRAAKQVFHFAAQVAVTSSLIDPTEDFEVNVRGTLNLLEALRKLECP
                     APLLFTSTNKVYGPVDDIPLQANGSRYCPLSPAAASISEERRLDFHSPYGCSKGAADQ
                     YVLDYARTFGLPAVVFRMSCVYGPHQMGTEDQGWVAHFLIRSIEGKPIVLYGNGLQVR
                     DLLYVDDLVDAFLLAHANIHSISGQAFNIGGGLGNTMSLLELIEMIDWIDGKRPQLRF
                     GDPRPGDQRYYVSDTRKFQAATGWAPKVCARQGIQQLYRWLAEGRSAGRPHPAAIEGI
                     HEILAS"
     gene            complement(7353..8471)
                     /locus_tag="VFA54_15875"
     CDS             complement(7353..8471)
                     /locus_tag="VFA54_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZS52348.1"
                     /translation="MSRNVLITGGAGFIGGHLATELLRHGYAVRALDCLTSQVHGQHR
                     QRPGYLNRDVELLTGDLRDETFVDQSVRGVDAVYHFAAAVGVGQSMYRIAEYTSMNNL
                     ATAVLLQALSRRRVRRLIVASSMSVYGEGLYRASDGSFRERVQRTPKQLTLGDWEIRD
                     ENGFPLTPVPTPETKPPCLASVYALSKFDQEKMCQIVGAAYGIEVVALRFFNVYGPHQ
                     ALSNPYTGALVNFAARLMNGKPPLLFEDGRQQRDFVSVYDAVRACRLALEKPEAAGGV
                     FNVGSGKARSVREVACLLGRVLGKGGLEPEVTGQYRAGDIRHCFADITLAWRVLGYEP
                     GLELEPGVAGLAQWLEGQIARDRVAEARSELASRGLVI"
     gene            9099..10235
                     /locus_tag="VFA54_15880"
     CDS             9099..10235
                     /locus_tag="VFA54_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZS52349.1"
                     /translation="MMPGTNTENVETKLDQICAEIIGPAAPEVDRTGSFPTESIAALK
                     NAGLMGAMSAREVGGLGLGLRTTAGIIRRVSRECGSTAMVLCMHYCGTAVLEAYGSQD
                     VRRAAAAGDHLSTLAFSEAGSRSHFWAPVSTARRNANGVLLDAKKSWVTSASKATAYV
                     WSSQPLEAPGFSTIWLVPASAAGLTVHGPFDGLGLRGNDSSPVSAAGVHVPESAMLGP
                     DGKGFDVMMGIVLPVFNVLNAACSVGLMEAAVERTTRHAANTRYSHITSSLADLPTIR
                     NYLARMQIKTDMARGLLEETLAAIEAGRGDTLLRVLECKAAAGETANEVLDMAMRICG
                     GFAFRKDIGVERYFRDARAAGVMAPTTDVLYDFIGKAVCGMQLF"
     gene            10245..11099
                     /locus_tag="VFA54_15885"
     CDS             10245..11099
                     /locus_tag="VFA54_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HZS52350.1"
                     /translation="MNDNVLLLGAVAYDPKVVTIWDGFKHYFTKRGLAFDYVLYSNYE
                     RQVEAHFSGHIHVAWNSPLAWLQSERIARALSRRAEAICMRDTDRDLTSLIIVRPEGV
                     GEISALRGKRVAVGARDSPQATLIPLSYIASHGLEPGRDFEVIAFDRLVGKHGDHIGG
                     ERDAVRALLRGEADAACLIDGNYLLFAQEGTLPSGAARILARTPAYDHCNFTVLENAP
                     PELIGRFRELLLEMSYADPEIRTLLDLEGLKQWQPARTSGYRALAAAVDRFGTINSFV
                     TTIVTRCS"
     gene            11090..12289
                     /locus_tag="VFA54_15890"
     CDS             11090..12289
                     /locus_tag="VFA54_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52351.1"
                     /translation="MQLDLHDLGFEEGGHLLVKRSLRQVPVGGEIAVTGAAPELEVHL
                     RAWCRAEGHQFRWEQRGQNGGCAIIARGAAETGRWAGSERAGMPDPSQPGAVVEHAPR
                     KWGLAARGAKVEAGSPEFDFRLTDKLEVWADDAARIYAQAVAAQWDPETAIPWNTDFD
                     IPGEIEDAVVQVMTYLIENETAALVVPSRFIAQLHPHFREVMQVLAIQAADEARHIEV
                     FTRRALLKRNALGLSTAGGQASLKTLLDEPDFAIASFLLSVLGEGSFLSLLWFIERFA
                     PDPVTGAVARLAAQDEARHVAFGLAHLRQHLSREPSFRGRLADAVRRRHDALRHTAGL
                     NAEVFDALILMASGSWDHEDLRQGHQRVMALTREMDEGRRHRLLRLGFSDEQAAELSG
                     LHTRNFM"
     gene            12395..14218
                     /locus_tag="VFA54_15895"
     CDS             12395..14218
                     /locus_tag="VFA54_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52352.1"
                     /translation="MKSSQTNRSRRSRSGGAPAQGTSHGEAANRPALRQWRKKAAIAL
                     AAGYALIAAWYIWARRSPNHSPAEGAALVQLTSRSGQELFPALSPDGKLLAYCAKGHI
                     FLEKIEDKTETDLTPDSTSQNWQPAFSPEGARIAFRSEREGGGIFAMPAAGGPAVKLT
                     EFGYNPVWSPDGKQIACATETFLDPSSRSTAGSSLWIIDVGTGNKRPLTSPQGIPDAI
                     EPNWSPHGDRIAYWSMQSGHAGIWTVSAAGANPIPVTHDASTNWNPVWSADGRYIYFT
                     SDRSSEMELWRVRIEEKSGKVMGEPAPIIREPGFSGFISFSRDGRRMAFIKSARSANL
                     FTVAFDARHERTTGRPAAVTQGTRLVFGPDLSPDGRWFAFFSWGQRENIFVAGIHRET
                     PRPLTNDAYQNRWPRWSPDGKRIAFHSNRSGSDQIWTVYADGSELQQITDSPEPAIFP
                     VWSPDGKFLAYSSVTSSFILDATKSWNEQRPTALPAWSEPPGSFQAFSWSPDGRKLAG
                     HLQSAGSASDTAGIVTYSVADRKYQRLTNAGSFPVWLSDSRRLLYAHGDKIVLTDTET
                     GDVHEVLTTAPPVLFGISRDDRLLYFTRVQVEADIWLSTLK"
     gene            complement(14256..15080)
                     /locus_tag="VFA54_15900"
     CDS             complement(14256..15080)
                     /locus_tag="VFA54_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HZS52353.1"
                     /translation="MSIPRTWWRFAFGLTFLSVVYALQRPFKEYPAVEYNDLPLPADW
                     QEDTEFVFARLMFPGGPLDGYRGRFDGDWRLGLSLWTQDYPRADRFFSNALRRLTRIH
                     VRSVEQAVNLEDGDEVYYWPWVYAVQVGEWGLTQNQAKVLREYLLRGGFFMADDCHGT
                     AEWAEFEKRMKWVFPERSFVEIDDNDPIFHTLYDLTDRYQIPGREHLDTGYKKDGRAP
                     HWRAIYDDKGHIMVAAVINSDVGDSWEWADDSWYPEKYSWLGVRLGVNYVIYAMTH"
     gene            complement(15513..15782)
                     /locus_tag="VFA54_15905"
     CDS             complement(15513..15782)
                     /locus_tag="VFA54_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52354.1"
                     /translation="MPTIRPLSSTAGNPAYLAALHRGDCVIYAFVGPARDERTGHHLL
                     HLGCIGGTVLGDTTHYEIPVRDETSKPGAAIIDDGNHPGIVSLIN"
     gene            15875..16555
                     /locus_tag="VFA54_15910"
     CDS             15875..16555
                     /locus_tag="VFA54_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZS52355.1"
                     /translation="MPAIEQPVSIPIGSIALSGDLAIPDNARGIVIFAHGSGSGRHSP
                     RNRFVAQVLQQSGLATLLMDLLTPQEEIFDSATGELRFNIQLLAARLAAATEWLAGRS
                     ATRSLEPGYFGASTGAAAALAAAARLPGRIGAIVSRGGRPDLAGSALRQVRAPTLLIV
                     GGNDRAVIALNEAARDQLLTESRMEIIPGAGHLFEEPGALEQVAFLARNWFDEHLGLN
                     RAQEEAAV"
     gene            complement(16614..17114)
                     /locus_tag="VFA54_15915"
     CDS             complement(16614..17114)
                     /locus_tag="VFA54_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZS52356.1"
                     /translation="MPENSFDIASKIDLQEVQNAIHQALKEVHTRFDLKNTKTRIELN
                     EKERQILLASDDEFKLRAVNDILQQKLVRRSVPLKGLTYGKIEPAAGSSVQQNISLQQ
                     GIPVEKAREIVKTIKNTKWKVQASIQGDVVRVTSRDRDTLQQVIKLLRDRDFGIDMQF
                     TNYRTN"
     gene            complement(17118..17711)
                     /locus_tag="VFA54_15920"
     CDS             complement(17118..17711)
                     /locus_tag="VFA54_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS52357.1"
                     /translation="MPRVLLADDSPHAQRMGAEILHQEGFEVLGVTDGREVLETLGRF
                     GPDLVLADASMPHHSGYEICERIKTDPALQHVKVVLLVGAFEPLDRAEAMRVRADGVL
                     HKPFEPSVVIQTISLLVQNLPSAPSAAAGAGQSRDNAAAAEKGVFEQAVRAAMDDDRI
                     DLDRIRGALTLAVESALPAFLDEVTARVMEALRRPKL"
     gene            complement(17874..19178)
                     /gene="bamD"
                     /locus_tag="VFA54_15925"
     CDS             complement(17874..19178)
                     /gene="bamD"
                     /locus_tag="VFA54_15925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZS52358.1"
                     /translation="MHATVRKLIVLVAAAAALTGCARRKKYENPIITNSQQPDKVLFD
                     RAIGDIERSRFEVARLTLQTLINTYPDSEYIAKAKLAIADSWYRQGGTNGLAQAEAEY
                     KDFITFFPTMEEAAESQKKVCQIHYQQMEKPDRDPQHAIKAEAECRQFLVQFPNSKFV
                     PQVEQMLREIQEVRAEAEFRVGQLYAKKGSYRAAATRLAGLTEQYPLYSKADEALWVL
                     GDAYGRMGKNFEDKSARAYSRLVREYPLSAYAPDAKDRLSDMKRPIPDPDQNALARMQ
                     YELENRETTGVFGHAFGIFSRKPDVKAAAKTGKPSLVAAAPVIPEGVNVAAPATGTAT
                     AEVGAQTITGPSALDTQPDARSRPPAAATPAPTTGDPGGSAASTASASATGSASAAAT
                     NEVSASTASSSGETVNGSAPSTTSGSAGSSKKKKKGRFLKIF"
     gene            complement(19199..19873)
                     /gene="rpe"
                     /locus_tag="VFA54_15930"
     CDS             complement(19199..19873)
                     /gene="rpe"
                     /locus_tag="VFA54_15930"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZS52359.1"
                     /translation="MVEIAPSILAADFSRLADEIQSVQKAGVSIIHVDVMDGHFVPNI
                     TLGPPVVSSLRRATNVTLDCHLMIEDADRYAPEFVAAGADMVSVHQEACPHLNRTLTE
                     IRNGGASPGVVLNPATPLCTLDEVLDLVDYVLIMSVNPGFGGQEFIPSTLEKVRRLKH
                     IRTERNLNFRIEIDGGISEKNAGDAARAGCDILVAGTAVFGKPDPGKAVAALLRAAEG
                     ALAIRA"
     gene            20138..20761
                     /locus_tag="VFA54_15935"
     CDS             20138..20761
                     /locus_tag="VFA54_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52360.1"
                     /translation="MRSYRLHLPLAKAVAAAAASWVAAFLLHAYILGLPAAHTTRNVC
                     FGCFAGLAYAHLFEYFSHEVAMHRGLAGLRFIRDGHLRHHRIFYGQNFRSRRPEDLQH
                     VTTEWYAFPVLMAPHYALLAAIYPAAAPAFFFGVTAHFIIYETGHWFTHVAENGFDRT
                     LACIPGLGALRARQVLHHQLHHARPSVNFNFTPPYGGDRLAKTYRKA"
     gene            20881..21909
                     /locus_tag="VFA54_15940"
     CDS             20881..21909
                     /locus_tag="VFA54_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS52361.1"
                     /translation="MTRKSLRRKLLAAHRLSRELWMIARGLASKRHPILAHIIPIRRC
                     NLSCRYCNEYDDFSKPVPVEEMFRRIDRLALFGTTVITISGGEPLLHPDLDSIIRRIR
                     SRGMIAGMITNGYLLTAERIQRLNKAGLEHLQISIDNLTPDEVSKKSLKVLDKKLQLL
                     AEHADFHVNINSVIGGGIRNPEDALVVARRALELGFTSTVGIIHDGWGQVRALSEQEQ
                     RIYRTIKELGKRSYARVNYFQENIAHGRPNNWRCRAGGRYLYICEDGLVHYCSQQRGY
                     PATPLERYTPQDLEREYYTKKSCAPYCTVSCVQQVATIDFWRDPQHIEPSRSVSEPLV
                     QISTAAGE"
     gene            22075..23199
                     /locus_tag="VFA54_15945"
     CDS             22075..23199
                     /locus_tag="VFA54_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52362.1"
                     /translation="MSELRQAVLPRMLLALFLVLSGRHGVSGQAPQAGQGQRARRAAG
                     PCSPVDPVYERIAKATGGQVLRLRGADLANNPNLLAATGNKSATIVSASGLLRPSESR
                     EFLAPVDSTVEDMTFSIFVECKGEIQAFRASGAAASEGPGITIANVSSGEVLAISRPE
                     TGVWRVRTSGSGSFSVLVGVDSPIQLHRFEFVKRGGRPGHEALFPISGQPVLGETQIG
                     LAGLSGPTGAAAFEVLAESGERLQMLSLAQGSPDAAADEFVGQVSLPARPFRIAIRGV
                     DPRGFPYQPVYPPLFHPQPVEVTFVAASGEPAPGARAMVDFVVRNVGRPAVFRIAAAV
                     GTGAIRNGVPQTLSLNTGETGMLTCRLPYRPARLRDRRFQ"
     gene            23335..23658
                     /locus_tag="VFA54_15950"
     CDS             23335..23658
                     /locus_tag="VFA54_15950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZS52363.1"
                     /translation="MARTLIERRKSPRYLCSHIVEIWHGRETTAGLLEDLSAEGAAIA
                     FEAPLDRDELVVLVAGGLQASARVRYCTRRENDYRLGLEFTSGSRWQPHQWRPDHLFL
                     RPSNE"
     gene            23853..25250
                     /gene="sthA"
                     /locus_tag="VFA54_15955"
     CDS             23853..25250
                     /gene="sthA"
                     /locus_tag="VFA54_15955"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HZS52364.1"
                     /translation="MKQYDLVVIGSGPAGHHGAIQAAKLGKKVAVVEAKSCVGGACIN
                     TGTIPSKTLREAVLHLSGFKQRGLYGGAFDGAANISMSDLLQRVQEVIRTEIGVFNHQ
                     FRRNGVEILNGRASFVDPHTIRVEGVDGEVQCQGQYILIATGTRPAHNDSIALDGSVI
                     FDTDALVHLPVLPKLLTIVGGGVIGVEYACMAAALGISVTLVERRPRILDFVDDQIVE
                     ALSYHMRSRGVTFRLGEEVAEVIKTPEGKVCAKLKSKKQIWSDSLLYAVGRQGNTDSL
                     NLAAAGLEADARGRLPVKENFQTAVPHIYAAGDVIGFPSLASVSMEQGRLAACHAFHH
                     PASAVPELFPYGIYSIPEISFVGATESRLTENDVPYEVGVANYREIARGQIIGDTHGM
                     LKLLFHRETRKLLGVHIIGEGATELIHIGQAALSFGGTIDFFVNTVFNYPTLAECYKV
                     AALAGLNRLNYSGSL"
     gene            25345..26523
                     /locus_tag="VFA54_15960"
     CDS             25345..26523
                     /locus_tag="VFA54_15960"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HZS52365.1"
                     /translation="MSGKTAVAGYTHDEVEELLAQGNPGGRLTKHDLATPALILDLDL
                     FESNLQKMAAFVRSRGRGFRPHAKTHKCPEIARRQLAAGASGVAVAKISEAEVFAKEG
                     IGGLLVTTAIVGARKIARAVRLAAARPETTFVIDNETNARELSEAAQAEGIRIKVALD
                     LYVGRRTGILPGRPALDLARQVAAMRGLDLEGIQAYAGQASHVIGFEERRRFSRECMS
                     QAVETRCLLEQAGIEVRMLAGGSTGTYNIDIELDGITELQPGSFIFMDVDYGRIGGPD
                     SDAYTDFAKALTVLATVVSRPESCKAIVDAGLKAFSTDKPFPPESKDLPGLLYGFAGD
                     EHGRLDFSHAGCELKVGDRLEFVIPHCDPTVNLYDRLYCVRGEKVEEVWRISARGMSQ
                     "
     gene            complement(26575..27777)
                     /locus_tag="VFA54_15965"
     CDS             complement(26575..27777)
                     /locus_tag="VFA54_15965"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS52366.1"
                     /translation="MLSALIFGPSRHLVFGIQRGCASVENLCVYKTLYAYPSIYEIVR
                     FLNAFTPKIIFLEIEAAQTAFDLAREVRNSSPETVIIGFAQRCGREKALEALEAGISE
                     ILIPPFDEDDFHQAIIQVLDSGKAGPPENLFAFVPAKAGSGATTTALNVAGALARDWK
                     KKILLVDGDLRAGVVCLLLNLNPERSVTDALEISNQLDQDHWMQLRSSAGGIDVLGAP
                     RIPSPTAFSKLSYHRFFRFVRSQYDAVIVDLPEGATELTEAVVTQAKDFYIVATPETA
                     SLFLARRKVLELRGIGVSRTRISVAVNQHREDDPPLAEMEKFLERRITAVLPEDDTSV
                     RRALRSGSVVDAASNLGSAIREFACTLAGCDLPPQPAEVPESPSFLDLLLGRAAPRRA
                     RAVSRGAA"
     gene            complement(28077..28916)
                     /locus_tag="VFA54_15970"
     CDS             complement(28077..28916)
                     /locus_tag="VFA54_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52367.1"
                     /translation="MNRATHRGLYLLRGRARLAFAIWLLLCAAGLSQPLRGVQSPRAL
                     FSQGVQAYAVEDYEGAIRLLEQAIAAEPAVSEYYQWLGKAAGRRAERSNILKAPGLAR
                     KARLAFEKAVELDRTNVGALNDLLDYYLEAPSALGGGEEKASAIASRLGAIDAAEGSR
                     ADGLIAVKRRDYARAEAGFRRALDLQPRNTGRLLDLASFLARRGRHAEADALFCRAAS
                     ADPNSPGLLFAWGKELGISGRDPQRARELLARYLQSERRPDDPPPSEVRNILKKFVDR
                     AGA"
BASE COUNT         5599 a         9261 c         8837 g         5680 t
ORIGIN      
        1 atgcaaaact tcgcgcgttt ggcgcggccg cgggatggat tctgcctgaa gacgccgccc
       61 cgccggtgaa tcgaggagag gctttgatct ggtccaaaaa tgagaagccc tggcgtgccc
      121 ggattattcc gggccgcgcc gaacggctga ggcatgcaag gaaatacgca gccggcgaaa
      181 tcggccccgg catggagttc tgctttcgcg ggccggaggg caagctcaat ctgcgggcca
      241 ccaacctgat taccttcgtg cagctcgccg ccggcgtcga tcccgaaacc tggtcgtacc
      301 atctgcgccg cggcgagtat tcgcgctggt tccgggacgc cattcgcagc gaagagctcg
      361 cgctggaggc cgagcgcatc gagaaagacc ggaagcttag cccggccgaa agcctcgccc
      421 ggatccggcg ggcgatcgag tcgcgcttta cattgccagg atgatcgcgg cgcacgcggg
      481 ccgggatggc gtgcaaacgc cgccggcggc gctttgccgg aagctggaga cctcctcttt
      541 ttatggcgaa cccggatgtg attgtcatcg ggtcgggacc caatggcctt gcagccgcca
      601 ttacgctggc tcaggctggc cgctccgttg tggtattcga ggcggaacac acgcccggcg
      661 gaggcgcccg ctccgcggag ttgacgctgc ccgggtttgt tcacgacgtc tgctccgccg
      721 tgcacccgct cgcgctcgcc tcgccgttct tccgaacgct gcccctcgcc gaacatggac
      781 tcgcatggat cgagccaccc gccgccctgg cccatccttt tgacgacggc acagccgccc
      841 tgctcgagcg cccggtccaa gctacggcac agcatctcgg cgaagaccag agcgcttaca
      901 tcaaatggat gacgcctctg gtggaggatt gggcgcggct cgaagcctcc attctcggcc
      961 cgccccgctg gcctcggcat cccttgaccc tggcccgctt tggcgcgagc gcgctccgcc
     1021 cggcttccgc cctcgcccgt tcgcttttcc ggggaaggag ggcgcgcgcg ctgttctccg
     1081 gctgcgcggc gcacgccatg atgcccctgg aaaagccgct cacggcgggt tttggcatgg
     1141 tgctggcgtt gctggcgcat gttgttggat ggccgctggc gcggggaggc acgcaaagcc
     1201 ttaccgacgc tctcatttct tacttgcgtt cgttgggcgg ggaggttctg acgggcgccc
     1261 gcgtcacttc gatcgacgag ctccctccgt cgcgcgcgat tctgtgcgac ctttctccga
     1321 agccgctgct gcaaatcgcc ggccatcgtc ttcccggcgc ataccggcgc aagctggagc
     1381 gctaccgtta cgggctggcg gcctataaga tcgattgggc gctcgatgga ccggtcccgt
     1441 ggcgcgcggc cgagtgcact cgggcggcca ccgttcattt gggcggcacg atggaggaga
     1501 ttgcagcctc cgagagggaa tgctggtccg gccggccccc ggaacgcccc tttgtgcttc
     1561 tggtgcagcc cagcttgttc gatccggccc gcgccccggc gggcaaacac acagcatggg
     1621 cgtactgcca cgtgccgaat ggctgctcat tcgaaatggc aagccgcatc gagcagcaga
     1681 tggaaagatt cgcgcccggc tttcgcgacc gtatattggc ccggcatata atgccgccgg
     1741 cggaaatcga aaagcacaat ccgaacctgg tcggcggaga catcgcaagc ggcgttccgg
     1801 atctgcgcca gttcttcgcc cgccctgcct ggagtctgta ttccactccg ataaacggcc
     1861 tctacatttg ctcggcgtcc acaccgcccg gaacgggcgt tcacgggatg tgcggctatt
     1921 tcgccgcccg gcgggcgctg aaaaacttgt ggggcggctg aaccgcgaac gcttttccct
     1981 cacgcgagcg cggacggaag ttcgctgcca gctcggtctc gatcttgcgc cgggcaaacg
     2041 gcgaagaagt cagcgaagtc tcgattcttc ccttgctttt gtctttccgc atcgcttgcg
     2101 tacgaatcag cacccttcgc cgcacaaaga gcttgattgt acattgctct gctcatacaa
     2161 tctccagatt gtaccttagg cgacgaaaca gggcctctgg atgttggtag acataccggc
     2221 gcctgccagc gcttcagcgg agcgggacga gcgcctccgg gagggcaaag gcggcctcgc
     2281 ttccccggtt tggcaagagc tgctgaaacc ctatcgcggc gcgtacagca aatacatcgc
     2341 cggggtcatc ttgcgccaag ccatactcgt gctcggcggc tattcgatgg tgtgggccat
     2401 acgcctttgc acggggcgcc cgaccttccc cgagtggctg gtgatcgcgg ctcttctcct
     2461 tttcgacagc gtctatctga gcctcgatac cggcctgaac acacttttca cgacacggct
     2521 ttcttttccg ctgttcggcc ggatgaaaat cgcggcgctc gaaaagattt ttcagatgcc
     2581 gacggagtgg catcaggagc agagctcggg cactttgctg ggcaaagtaa acaacggcgt
     2641 tggacgagtc atgggcatgg gagaggcggt gagccgcgaa ctgctcccgg ccgcgattcg
     2701 cactatcctg agccttgttc ctctgttgct cctgagcctc tacactgcgc ctttgctgct
     2761 gctcgcgctg ggcttgttta gctggctcac gctccgcgaa aacaatcgcc gcgcggcgtt
     2821 gcggagcaaa cggcacgcct gctatgtccg cgactctggt aaattcggcg agtatgtaca
     2881 ggcggcgcag ccggtcgtcc aattcggcca gacacggcgg ctcctcgcag actacgggcg
     2941 gctgcaagaa cgcatcgtca gactcggccg agcggaaatg cgcgtagcga atacgtattc
     3001 ctggcggcgc agcctggtgc tctcgctggc caaacgcgtt tcgcagggaa tctggatctg
     3061 gcagcttcgc cgcggcgccc tggacgtggc tatggccatg tacctcaacc tgctcaccga
     3121 ggagctgctg gcgtcgttct ggggctatgc cggtttgctc gagcgaatct tcgaagggct
     3181 cgagcccgcc aggatcctgg tgaaactttt gaacgaacaa cccgccgtca gggatcagcc
     3241 aggcccggcg ccagctcccg ccgaagggga ctgcggcatc gagatgatcg atgtccagtt
     3301 ttcctactca cgcggcaagc acatcgtccg caacctgaac ctctccatca aacggggcga
     3361 ggtgctgggc atcgtgggcc gttccggatc gggcaaaaca accattcaga atcttctctc
     3421 gcgcatcttc gaggcgcagc acggcaaaat taccgtctac ggcgccgata tccgccaatg
     3481 gcccctgggc cgcttacgcg gactttttgc ctgcgtatcg cagaatggag gcgtgttttt
     3541 ctccggcatg actctgttcg atacgatccg cttcgcgcgg cccgaggcga cattccgcga
     3601 agtagttgaa gcggccaaat gcgcctgcat tcatcaggac atcattcgca tgcccgccaa
     3661 ataccgcacc aaaatgaaag ccggcggggc gaacctctcg aaaggacaac agcagaggat
     3721 cgccctggcc caagctctcc tggcgctgaa agacgaccgt aaaatactcg tgctcgacga
     3781 atttacgagc cagctcgatt cggaaaccga atccaagatc ctggccaacc tgctgccgca
     3841 cctggcaggc aggaccgtaa ttctcatcgc tcaccggctg tctacgctgc ggcagatcgc
     3901 cgaccgaatc gccgtgctgg aagatgggag gattatcgag caaggcgccc acgaggagct
     3961 tgtgcggtca aatggctggt atgcggaaat ggcccgggcg caggccgtca cttagtccag
     4021 gattgcggcc cggcggccga cctttcgagc tgttcgagcg cgagcggtcg ttcctcctga
     4081 atatcgctga actcagaaaa cgcagctaga tattcgcgat aggctcgttc ccaggccaca
     4141 tcgtccggag ctccccagcg ccgcagatac tccggcgacc caggataggg aaacagcggc
     4201 accggccgat tggcccaaac accgtgctgc cgcagctccg cgcgccatgc ttccacctcg
     4261 gccgggttat cgttttgcga ctccagcaaa ttggcctgga caaaggggac gcgctgcctg
     4321 gcataaatca gccgttcggc aaggtcggcc gtagacagct tgcaccgctt atcgagcaga
     4381 gcgcgcccgt cttctgtgat gctttccacg cccgcttcga tcgaaacgca gcctgcccgc
     4441 cccaacagat caatcatggg cggcgcccaa agatcaatgc gcgtttgaat tccgaaccgg
     4501 atcttccggc gggccagagc ttcgagcaga tccgcgtacg gcaaaaagat ttcgtcgatg
     4561 aaataaatgt actcgacgcc tttcgccagc aacccgtcca gttccaccag caccgcgcac
     4621 agcgggcgcc ggcggaagct gtttcgaaag ttatctttcg cgcagaaggt gcagtgataa
     4681 gggcatccgc gtgtggtttc catttccgcg ccgggaccgc tgggcgccgc gtcgaagcga
     4741 tgatgctggt gccggtgcag ccgtatcgcc tcctgaggcc agtcgagagc cggcagccgc
     4801 gccatgtcgc aggcatgagg cccgccctgg attacgggct ccccgtgatc cagatagcac
     4861 aaggaaggaa tttctttcca atcaccggca agtttcggca agatttcttc gcactcgccc
     4921 agcaccacgg cgtcgggctc gagctttcgc aggacggcgc gcggcgttgt cgaaccgtgc
     4981 ggcccgacta cggcgatttt cccggcaagg tcccgtaccg cctgcaacgc ctccagagga
     5041 acgcgaagct ccggcggcgc acagcgccag aaaaggtagc tcggagcggt ggtgaccacc
     5101 agaaagtcag gccgatacgc cgccgccgcg gcccgcactt gttccagaga aaggttctcg
     5161 agttgcgcgt cgattatcac cgcctcgtga tgccgttgcc gcaacaacgc ctgggcatat
     5221 ccgtattcca ggggaaggtg cggatagcgg cacccgaaat agatgcttcc ctcgaacgac
     5281 cagttggggt taagaagcaa gaatctcatg gatgccctcg atcgcagccg gatgaggccg
     5341 tcctgcgctt cttccttccg ccagccagcg atagagctgc tggattccct gacgggcgca
     5401 taccttgggc gcccatccgg tggcggcctg gaactttcgc gtatcggata cgtaatatct
     5461 ctggtcgccc ggccgcgggt ctccaaaccg tagctggggt cttttgccgt cgatccagtc
     5521 gatcatctcg atcagttcga gaagactcat cgtgttgccg agcccgcctc caatgttgaa
     5581 cgcctgcccg gagatggagt ggatattggc gtgggccagt agaaatgcgt ccacaagatc
     5641 gtccacatag agaagatcgc gaacctgcaa gccgtttccg tagagcacta taggcttgcc
     5701 ctcgatcgag cgaatgagga agtgcgccac ccagccttgg tcttccgtgc ccatctggtg
     5761 cggcccgtaa acgcaactca tccggaatac gacggcggga agtccgaagg tccgcgcgta
     5821 atcgagcacg tactggtctg ccgctccctt tgagcaaccg taggggctgt gaaaatcaag
     5881 ccggcgctcc tcgctgatgc tcgcggccgc ggggctcagg gggcaatacc gcgagccatt
     5941 ggcttgcagc ggaatgtcat ccaccggccc gtagaccttg ttagttgatg taaacagcag
     6001 cggtgcaggg cactccagtt ttcgcagcgc ctctagcaga ttcaaggtgc cccgcacatt
     6061 cacctcgaaa tcttcagtgg gatcgatgag gctgctggtg acggcgacct gggccgcgaa
     6121 gtgaaaaacc tgtttggccg cccgcaccgc ggcgcgcagt gcggcggcgt cccgcacgtc
     6181 ggcggcttcc aggtgaacgc ggtctccata tcgctcttgc agccaatgaa ggttccgctc
     6241 ggttccgggg cgggacagat tgtcaaatag caagaccgat tggccgccgg aaagcaggcg
     6301 gtgagccaaa ttggcgccta gaaagccggc gcctccggtg atcaggacgt gccgtcgccg
     6361 ccccgtggaa cgagcgcgct ggtccgcgcg ggccttgtcg cgaacttctt cgatcccgcc
     6421 gcgggcccag acctgaaaca acagctttga tttcccgtct ccctgcttca ggccaaaatg
     6481 gcagtcgcgt tcgtcggtgc gcagagcgcc gccttccgca gcgcggtcct gaagcgcttt
     6541 ccagtaaact cgcggcacgg gcgcttccac agcttcagca aaggctcgga gctgggctcg
     6601 ctcatcctcg cgccaggtcg agtagcccgc ttccgtgatc cagatttgcg ccgccgatcc
     6661 gccttcggca agggcgcttt gcgtgtcctg gatgtgtttg gtccagccgc tccaagggtg
     6721 ctcggagacg ccgggaaacc catgaatgcc tacggcgtca acataggcca tcacgccccg
     6781 gtcaaacata agtttcagcc accggcagtc gggcggccac gcgccgggca gcacagtttt
     6841 cttgccgcgc ttgcgggccc agtacgccgc cccgccgatc atctcacaga agatctccca
     6901 accggggtcg aggcgataat cccagtagtc gatgttgttc ggctcggtcc agaattcgat
     6961 ccattcgaaa agcctgccga aacgcgtgat cattacatcc agaaagtcgg cgtacgcttt
     7021 gggcgttctg gggggagagg agatcttcgg cacgatgccg agcgaaggcg gcgtgtaaag
     7081 aaaacagggg aggatcgaga cttctttggc gagtctcggc agcagccagg cataccaccc
     7141 atctccttcc gaccgatacc agtcggccca gcaaatcgtg gtgcgcaaat cctcgactcc
     7201 caggctcttc aaatcggcca gcgtggcttc gacgcgctca tattcgcccg ggaagaagca
     7261 ttccaccatg ccgactctct gcccgccgca cgccggtccc gcttttcgcg gcgtctcctc
     7321 gtggtttcgc gctcgctggc cgggcggcct cttcatatca ccagccccct cgatgccagt
     7381 tcactgcggg cctctgccac gcggtccctg gcgatttgcc cttcgagcca ctgcgccaaa
     7441 cccgccacgc ccggctcgag ttccagccct ggctcgtaac cgagcacgcg ccacgccagg
     7501 gtgatatccg caaagcagtg ccggatatca ccggcgcgat actggccggt cacctccggc
     7561 tcgagccctc ctttgccgag aacgcgtccc agcaagcagg cgacctcgcg cacggaccgc
     7621 gcttttccgc tgcccacgtt gaatacgccg ccggcggcct ccggcttctc caacgccagc
     7681 cggcaggccc gcaccgcgtc gtacacgctg acaaagtcgc gctgctgccg cccgtcttca
     7741 aataaaagcg gcggctttcc attcatcagc cgcgcagcga aatttaccag cgcgcccgta
     7801 tatgggttgg acagcgcctg gtgcggtccg tagacattga agaaccgcag cgccactacc
     7861 tcgattccat aggccgcgcc tacgatctgg cacattttct cctgatcaaa cttcgaaagc
     7921 gcgtagaccg aggcgaggca cggcggtttg gtttcgggcg tagggacggg agtcagcggg
     7981 aagccgtttt catcgcgaat ttcccaatct cccagggtaa gctgcttggg agtccgctgt
     8041 acgcgttcgc gaaagctccc atcggaggcg cgataaagac cttcgccata gacgctcatg
     8101 cttgacgcga ctatgagacg ccgcacgcgg cggcggctga gcgcttgcag cagcacggcc
     8161 gttgccaggt tgttcatgct ggtgtattcg gcgatgcggt acatactctg ccccacgccg
     8221 accgcggcgg cgaaatggta cacagcgtcc acgccgcgca cggattggtc cacaaaagtc
     8281 tcatcccgca ggtcgccggt gagcaattcc acgtcccggt tcaggtagcc gggccgctgc
     8341 cgatgttgtc cgtggacttg ggaagtgagg caatcgagag cccgcacagc atatccgtgc
     8401 cgcaggagct cggtggcgag atgaccgcct ataaatccgg cgccgcctgt gatcaatacg
     8461 ttcctggaca tcactgattc gccgatcaac gaagatcgcc gcgccacgtc acgcccgttc
     8521 cctcgcaaaa caagcgctca attgcgttgc aagagcggta ccaatattgg cgcgaaccgt
     8581 attgcaaaac agcttcttac cctgttttcg tacacccaaa cctgtgttgt ggcatcgaac
     8641 cactcttgaa gcatctgcca gccgagggcc ctcgatgtca ccggcggtcc ggaatcccca
     8701 gggcgttcga aactccgcat ttcggatctg gatcagaaag ccattgtcta aatgccggcc
     8761 gcaaagaccg cgcgagttcc ttcctggccg cgccgctgcg aaagatctac tcacctccgg
     8821 ccataacacg gccgttcgtc cggccacaat taccagaaac cgggcagcca gacaccgcgt
     8881 ttccgccgag agcgcccgct ccgcctgaat ccgcaaaagc ccctaacggc gggccggctc
     8941 agtgctacac tggcattcgt atgacagcgg ttgtttatca aatctgaatt ccattcacgg
     9001 gctcgacggg atcgagcctg gccgccgacc ctgccggtgt gaattgaggc taacctcctc
     9061 gccgcgccgg tttgcgggac ggagtgaggg aaaactcaat gatgcctggt acaaatactg
     9121 agaacgttga gacgaagctc gatcagatct gcgcggagat catcggccct gcggcgcctg
     9181 aggtcgatcg gactggcagc tttccgaccg aatccattgc agcattgaag aacgccggat
     9241 tgatgggcgc catgagcgcc cgcgaagtgg gcgggctcgg cctcggattg cgaaccacgg
     9301 ccggcatcat ccgccgcgtt tcccgcgaat gcgggtccac cgcgatggtg ctctgtatgc
     9361 attactgcgg gacggcggtg ctggaggcct acgggagcca ggatgtccgg cgagccgcgg
     9421 cggcgggcga ccatctcagc acgctggcct tcagcgaagc ggggtcgcgg agccatttct
     9481 gggcgccggt cagcacagcc cggcggaacg ccaacggcgt cctgctcgac gcgaaaaaga
     9541 gttgggtcac ctccgcatcc aaggccaccg cctatgtgtg gtcgtcccag ccgctggaag
     9601 cacccgggtt cagcacaatc tggctcgttc ccgccagcgc cgcagggttg accgttcacg
     9661 gcccattcga cggcctgggg ttgcgcggca atgattcctc gccggtctcc gccgccggcg
     9721 tgcacgtgcc tgaatcggcg atgttgggac ctgacgggaa aggttttgac gtgatgatgg
     9781 gcatcgtgct gccggtcttt aacgtgctca acgcagcctg ctcggtcgga ttgatggaag
     9841 ccgccgtcga gcgcaccacg cggcacgcgg ccaacacccg ctactcccat atcacatcga
     9901 gcctcgccga cctgccgacg atccgcaatt atctggcgcg gatgcagatc aaaacggata
     9961 tggcccgggg tttgctggag gagacccttg ccgccatcga agccgggcgc ggcgacacgt
    10021 tgctccgcgt cctggagtgc aaggcggctg ccggagaaac ggcgaacgag gttctcgata
    10081 tggcaatgcg gatctgcggg ggcttcgcgt tccgtaaaga catcggcgtg gagcgttatt
    10141 tccgtgacgc gcgcgcggcc ggagtcatgg ccccgactac cgatgtactc tacgatttca
    10201 ttggcaaggc cgtctgcgga atgcaactgt tttgagggaa atggatgaac gacaacgttc
    10261 ttctcttggg agcggtcgcg tacgacccga aagtcgttac tatctgggac ggcttcaaac
    10321 attacttcac caaacgcggc ctggcctttg actacgtcct ttactcgaac tacgaacggc
    10381 aggttgaggc gcacttctcc ggccatattc acgtcgcctg gaactcgccg cttgcgtggc
    10441 ttcagtcgga gcgcatagcc agagccttga gccggcgggc ggaagcgatt tgcatgcgcg
    10501 atacggaccg cgatctgact tcgctcatca tcgtccgtcc ggaaggcgtg ggcgagattt
    10561 ccgctctgcg gggcaagcgg gtcgccgtgg gcgccagaga ttcgccgcaa gccactctga
    10621 tccctctcag ctatatcgcc tctcacggtc tggagccagg ccgggatttt gaggtaatcg
    10681 ccttcgaccg cctggtgggc aagcatggcg accacatcgg cggagagagg gacgcggtgc
    10741 gcgctctact gcgcggcgag gccgacgccg cctgcctcat cgacggcaac tatctcttgt
    10801 tcgcccagga aggcacgctc ccttcaggcg ccgcgcgcat cctggcgcgg acgccggctt
    10861 acgatcactg caactttacg gttctagaga atgctccgcc cgagctgatc ggccggtttc
    10921 gcgaattgtt gctggaaatg tcctacgccg atcccgagat ccggacgctg ctcgatctgg
    10981 agggcctcaa acagtggcag ccggcgcgca ccagcggtta ccgcgccctg gcggccgcag
    11041 tggatcgttt tggaactatt aactcgttcg tcacgacgat tgtcacccga tgcagctaga
    11101 tttacacgat ctcggatttg aagaaggcgg ccacctgctc gtgaagagaa gcctgcgcca
    11161 ggttcctgtg ggcggcgaaa ttgcggtcac aggcgcagcg ccggagctcg aagtccacct
    11221 gcgcgcctgg tgccgcgcgg aaggccatca attccgctgg gagcagcgcg gccaaaatgg
    11281 aggctgcgcc atcatcgccc gcggcgcggc ggaaacaggg cgctgggccg gatcggagcg
    11341 ggctggtatg cccgatcctt cccagcccgg cgctgtcgtc gagcatgccc cgagaaaatg
    11401 gggcttggcc gcgcgcggtg cgaaggtcga ggcgggaagc ccggaattcg acttccggtt
    11461 gacggacaag ctcgaggtgt gggctgacga tgccgcgcgc atctacgcgc aggcggtcgc
    11521 cgcgcagtgg gacccggaga ccgccatccc ttggaacacc gatttcgaca tccccgggga
    11581 gatcgaagac gccgtcgtcc aggtcatgac ctacctcata gagaatgaga cggccgcgct
    11641 tgtggtcccc agccgcttca tcgcccagct ccacccgcat ttccgtgaag tcatgcaggt
    11701 tttggcgata caggccgctg atgaggcccg gcacatcgag gtttttacca ggagggcgct
    11761 actgaagcgc aacgcgctcg gcctttccac tgccggcggc caagcttccc tcaagacttt
    11821 gttagatgag ccggacttcg ccatcgcgtc ctttttgctt tcggtgctgg gcgagggaag
    11881 ttttctttct ctgctttggt tcattgaacg tttcgcgccg gatccggtca ccggagcggt
    11941 ggcgcggctg gccgcgcaag acgaagcgag gcatgtcgcc tttggtttgg cccacctgcg
    12001 ccagcacctg tcgcgcgagc cttcttttcg cgggcggctt gccgacgcag tgcgccggcg
    12061 gcacgacgct ttgcggcaca cggccggcct gaacgccgaa gtattcgacg cgctgatttt
    12121 gatggcctcc ggatcctggg atcacgaaga tttgcggcaa ggccaccagc gcgttatggc
    12181 gcttactcgc gaaatggatg agggccgcag gcatcgcctg ttgcgtctgg gtttttcgga
    12241 cgagcaagcg gccgagctct ccggcctgca cacgcgaaac ttcatgtagc ccggcccagc
    12301 ctgtcgatcc cggcttcgta gtcgagaaac gggacgctcc gcggcgccaa gggccgcctg
    12361 gcggccgtat ccggagaggt cctgaaaacc gttcgtgaaa tcatcgcaaa caaaccgtag
    12421 ccgccggtcc cgctccggag gggctccggc ccagggaacc agccacggtg aagccgcgaa
    12481 ccggcctgcg cttcgccagt ggaggaaaaa ggccgccata gccctggccg ccggatacgc
    12541 gctgatcgcg gcctggtata tctgggcgcg gcgcagcccg aaccactctc cggccgaagg
    12601 cgccgcgctt gtacagctca ccagccgcag cggccaggag ctctttccgg cactttcgcc
    12661 ggacggcaag ctgcttgcct attgcgcaaa gggacatatc ttcttagaaa aaatcgaaga
    12721 caagactgaa acagacctga cgccggattc cacgtcacag aattggcagc ctgcattttc
    12781 tccggaggga gcgcgcatcg ccttccgctc ggaaagggag ggcggcggta tcttcgcgat
    12841 gccggcagcc ggcggccctg ccgtgaagct cactgaattc ggttacaacc cggtttggtc
    12901 gccggatgga aaacagatcg cctgcgcaac cgagactttt ctggatcctt cgtcgcgttc
    12961 cacggccggc agctcgcttt ggataatcga tgtgggtacc ggcaataagc gccccctgac
    13021 aagtccgcaa ggcattccgg acgccatcga gccaaattgg tcgccgcacg gtgatcgtat
    13081 cgcatattgg agtatgcaat ccggccacgc cggtatctgg accgtctctg ctgccggggc
    13141 taatcccatt cccgtcactc acgacgcttc caccaactgg aatccggtgt ggtccgccga
    13201 tggccggtac atttacttca ccagcgaccg tagctcggaa atggagttat ggcgcgtgag
    13261 aatcgaagaa aaatcgggga aggtgatggg agaaccggcc ccgatcatcc gggaaccggg
    13321 gttcagcggc tttatcagct ttagccgcga cgggcggcga atggctttta tcaagtcggc
    13381 gcgttctgcc aatctcttca cggtcgcgtt tgacgcgcgg cacgaaagga ccaccggccg
    13441 gcccgcagcc gttacgcaag gaacaaggct cgttttcggt ccagacctgt cgccggatgg
    13501 aagatggttt gcgttctttt cgtgggggca gcgggaaaat attttcgtgg cgggaattca
    13561 tcgggaaacg ccccgcccgc tcacgaacga cgcctatcag aaccgctggc cccgctggtc
    13621 gccggatgga aaacggatcg cctttcattc caatcggagc ggcagcgatc agatttggac
    13681 cgtgtacgcc gatggcagcg aattgcaaca aatcaccgat tcgcctgaac ccgccatctt
    13741 tccggtttgg tcgccggacg ggaaatttct ggcttacagt tccgtcacaa gctctttcat
    13801 tctggatgca acaaagtcct ggaacgagca gcggcccact gcgctccctg cttggagcga
    13861 acctcccgga agctttcaag ccttctcctg gtcacctgat ggccgcaagc tggcgggaca
    13921 tcttcagagc gccggcagcg catcagatac cgcgggcatc gtgacgtact ctgtggccga
    13981 tcgcaaatat cagcggctca ccaacgcggg cagctttcct gtgtggctca gcgacagccg
    14041 gcgtctgctc tacgcgcatg gggataagat cgttctcacc gatacggaaa ccggcgacgt
    14101 gcacgaggtc cttacgacag cgccgcccgt gttgttcgga atttcccgcg acgaccgctt
    14161 gctttatttc acgcgggttc aggtcgaagc cgacatctgg ctttccacgc tgaagtaaca
    14221 ccgcccgcaa tcccggagag ctgcttcgcg ggaaatcagt gcgtcatggc gtaaatgacg
    14281 taatttacgc ccaggcggac ccccagccaa gaatatttct ccggatacca ggaatcatcc
    14341 gcccactccc aggaatcgcc gacatcggaa ttgatcacgg cggccaccat gatgtggccc
    14401 ttatcgtcgt aaattgcgcg ccaatggggc gctcggccgt ctttcttata tccggtgtcc
    14461 agatgctccc ggcccggtat ctggtagcgg tcggtcaaat cataaagagt gtggaagatc
    14521 ggatcgttgt cgtcaatctc cacgaaggaa cgctccggga acacccactt cattcttttt
    14581 tcgaactccg cccactccgc ggttccgtgg caatcgtccg ccatgaagaa gccgccccgc
    14641 agcaggtact cacgcagcac tttagcctgg ttctgcgtca agccccactc gcctacctgg
    14701 accgcgtaga cccagggcca atagtacacc tcgtctccat cttccaggtt cacggcctgt
    14761 tcgacggagc gcacatggat gcgcgtcaga cgccggagcg cgttggaaaa gaagcggtcc
    14821 gctcgcggat aatcctgcgt ccagagcgaa agtcccagcc gccagtcccc atcgaagcgc
    14881 ccgcgatagc cgtcaagcgg tccgccggga aacataagcc tggcgaagac gaactccgtg
    14941 tcttcctgcc agtcggccgg cagaggaagg tcgttgtatt ccactgccgg atattctttg
    15001 aacgggcgct ggagcgcgta tactacactc agaaaagtaa ggccgaacgc aaagcgccac
    15061 cacgttcgag ggatgctcat ggccgccgcc ttcctcttag agaatatcac ctcgcggcgg
    15121 acgaccacgg cgcaacctgg attgagcaga gcggaacggc gatgccgccg ctgtggaatg
    15181 ccagacgggg ccggcggcac gcccggccga acaccagatt gcttggtttt gccgcttcgg
    15241 cgatttggaa cttggccgac attggctaga accttgctga gactccagtc gcggagcaca
    15301 ttttccgtcg ctcatgaaat cgcgaaaaca gaacaccggt cttccgacct ggatcgcttg
    15361 ccgcacttgc tttagcggct gccaaatgta ccgtaaaaac ccgctaaacc ggcggacgcc
    15421 tgaccacgga gacctccatg cagaaaatgc gaggacctga ggacatccag cctggcctgc
    15481 tgccttccgg acgacggcgc caggaggacg gctcaattga taagggacac aatgccgggg
    15541 tgattcccgt cgtcgatgat tgcagcgcca ggcttcgagg tctcgtcacg gaccgggatc
    15601 tcgtagtgtg tggtgtcgcc gagaaccgtt ccgccgatgc aacccaggtg cagcaagtga
    15661 tgaccagtac gctcgtcacg tgccggcccc acgaaagcat agatcacgca atcgccgcga
    15721 tggagcgccg ccaggtacgc cggattgccc gcggtagacg acagcgggcg tatggtcggc
    15781 atcattgcac aggccgacat cgccgcccgc atggaggagc cggcgacaac ggccgaggcc
    15841 gtcgccgaaa tctcatcgtc cgaaggaggc cctgatgccg gcgattgagc agccggtttc
    15901 cattccgatc ggctccatcg ccctttcagg cgacctggcg attcccgaca acgcccgagg
    15961 catcgtgatt tttgcccatg gcagcggcag cggccgccac agcccgcgca accgtttcgt
    16021 ggcgcaggtg ttgcaacaga gcgggctggc tacgcttctg atggatcttc tcacgccgca
    16081 ggaggaaatc ttcgacagcg cgacgggaga actgcgcttc aacatccaac ttctggccgc
    16141 acggctggcc gcagctaccg aatggctcgc tggccgctcc gccacgcgat ccctcgaacc
    16201 aggctatttc ggcgccagca cgggcgccgc agccgcgctg gcggcggcgg ctcggctgcc
    16261 gggccgcatc ggagccattg tttcccgcgg cgggcggccc gatctggcgg gctcggcatt
    16321 gcgacaggtt cgcgcgccca cgctcctgat cgtcggcgga aacgatagag cggtgatcgc
    16381 tctcaatgag gcggcgcgag atcagctcct cacggagagc cggatggaaa ttatccccgg
    16441 cgcgggacac ctttttgagg aaccgggagc gcttgagcag gtggctttcc tggcccgcaa
    16501 ctggtttgac gagcatctgg gcctgaaccg ggcccaggaa gaggctgcgg tttgaatgct
    16561 gcgtattctg cggcgccgca gaagggcgtg ccaggcggcg ttgacaaagc gtttcagttg
    16621 gtcctgtagt tggtgaactg catgtcaatg ccgaagtccc tgtcccgcag cagcttgatc
    16681 acctgctgga gcgtgtcgcg gtcgcggctg gtgacgcgaa ccacatcccc ctggatggag
    16741 gcctgaacct tccatttggt gttcttgatg gtcttcacga tttcccgcgc cttttcgacc
    16801 ggaatgcctt gctgcaagct gatgttctgc tgcaccgagg agccggcggc cggttcgatc
    16861 ttcccatagg tcagcccttt gagcgggacg gagcgccgca cgagcttctg ctgcaaaatg
    16921 tcgttgactg cccgcaactt gaactcgtca tccgacgcca gcagaatctg tcgctctttc
    16981 tcgttgagct cgatgcgcgt ctttgtattt ttcagatcga aacgggtgtg aacctccttg
    17041 agcgcctgat ggatcgcatt ctggacttcc tggagatcga tcttggaggc gatatcaaaa
    17101 gaattctcgg gcatagctca gagcttgggc cgccgcagcg cctccatcac ccgcgcagtc
    17161 acttcgtcga ggaaagccgg cagagcggat tcgactgcca gcgtcagggc gcctcgaatc
    17221 cggtcaagat cgatccggtc gtcatccatc gccgcgcgga ctgcttgctc gaaaacgcct
    17281 ttttccgccg cggccgcatt gtcccgggat tggccggccc ccgctgccgc cgacggagcg
    17341 gagggcaggt tttgcacgag cagactgatg gtttgtatta cgaccgacgg ctcaaacggc
    17401 ttgtgcagaa ctccatcggc gcgcacccgc atcgcctccg cccggtcaag cggctcgaac
    17461 gcgccgacca gcagaaccac ctttacgtgc tggagcgcgg ggtccgtttt gatgcgctcg
    17521 cagatctcgt agcccgagtg atgcggcatg gaggcgtcgg ccagcaccaa atcgggaccg
    17581 aagcgcccta gcgtctcgag aacctcccgg ccgtcggtga cacccagaac ttcgaatcct
    17641 tcctggtgca gaatctcagc gcccatgcgc tgggcgtgcg gactgtcatc ggcgagaaga
    17701 actctcggca cggatcgttc tccgactcag catgaatgga tagggaaacg ccgccatgct
    17761 cgcgccgcac cgcttccggc tcgcctccgc agaaccggcc gctcttacgc ccacggatcg
    17821 ttcactgact ccgccgagaa ccgtgcctcg gcgccaaggc gcatgccccg aaatcagaag
    17881 atctttagaa accgcccctt cttcttcttt ttggaagagc cggccgaacc cgaagttgta
    17941 ctcggagcag agccgtttac cgtctcaccc gagctgctcg ccgttgaggc tgagacttca
    18001 ttcgtagcag ccgctgacgc tgacccggtg gccgatgcgg acgctgtcga agcagcgctt
    18061 ccgcctggat caccagtggt gggcgccggc gtcgctgcag ccgggggcct ggagcgggcg
    18121 tcgggctgcg tatccagagc gctcggcccg gtgatagtct gggcgcccac ttcggctgtc
    18181 gccgttcctg tggctggcgc cgcaacattg acaccctctg ggatcacagg agcggcagcc
    18241 accagcgacg gcttccccgt ttttgccgca gcttttacat caggcttccg gctaaatatt
    18301 ccgaaagcgt ggccgaatac gccggtagtt tcccggttct cgagttcata ttgcatgcgc
    18361 gccagcgcgt tttgatcggg atcgggaatc ggccttttca tatccgacag ccggtctttg
    18421 gcgtcgggcg cataggcgct gaggggatat tcccgcacca gccgggagta agcccgggcg
    18481 ctcttgtctt cgaaattctt gcccattcgc ccataggcat cgccaagcac ccataacgct
    18541 tcgtccgcct tgctataaag cggatactgc tcggtcagcc cggcgagccg ggtggccgca
    18601 gcccggtagc tgcccttctt cgcgtacaat tggcctaccc gaaattccgc ctcggcgcga
    18661 acctcctgga tttcccggag catctgctcc acttgaggaa caaacttgct gttcgggaac
    18721 tgcacgagga actgccggca ctcggcctcg gccttgatgg catgttgagg gtcccggtct
    18781 ggcttctcca tctgctgata atggatttga cagacctttt tttgcgactc agcagcttcc
    18841 tccatagtcg ggaaaaaagt aataaagtct ttgtactcgg cttcggcctg ggcaagtccg
    18901 ttggtcccgc cctgacgata ccaactgtcc gcgatcgcca gcttggcctt ggcaatgtat
    18961 tcgctgtcgg gataagtatt aatcagcgtt tgcagcgtga gcctggccac ctcgaagcgg
    19021 ctccgctcga tgtcgccgat ggcccggtcg aaaagaactt tgtccggttg ctgcgaattc
    19081 gtgatgatcg gattttcgta cttcttccgg cgggcgcacc cggtcagggc cgccgccgct
    19141 gctaccaata cgattagttt tcgtacagtt gcgtgcatag acaaacgcct tccgaaactc
    19201 aggcgcggat cgccagcgcc ccctccgcag ctcgcaacag cgccgccacg gccttgccgg
    19261 gatcgggctt tccgaagacg gctgtgccgg ctaccagaat gtcacagccg gcccgagccg
    19321 cgtcgcctgc gttcttctcg ctgatgccgc cgtcaatctc aatccggaag ttcaagttgc
    19381 gctcggtgcg aatgtgcttc agacggcgaa ccttctccaa cgtactggga atgaactcct
    19441 gccccccaaa acccggattg acactcatga tcagtacata atctaccaga tcgagaacct
    19501 catcgagcgt gcacagcggc gtcgccgggt tcagcacgac gcccggggat gctcccccgt
    19561 tccggatctc cgtcaacgtg cggttcaaat gggggcaggc ttcctggtga acggaaacca
    19621 tgtccgcgcc agcggccaca aactccgggg cgtagcggtc tgcgtcctcg atcataaggt
    19681 ggcagtccag cgtgacgttt gtggcgcggc gcagcgaact gaccacggga ggccccagag
    19741 tgatgttggg cacaaaatgg ccgtccatta catccacatg gatgatcgag acccccgcct
    19801 tctgcaccga ctggatttca tcggccagcc gcgaaaagtc agccgcgaga atcgaaggtg
    19861 caatctccac catgacgcgg taggttttca gtttagcaca gccagccctc ctgcccgatc
    19921 ccacctgtag cgcgcgcctg gtgcagggcc attttttgtc aaaccggagt ctgcttcgtg
    19981 cgcatcgaga ccggctgacg ggcatgttcc cgccagggtc gcatggggca gcaagaatga
    20041 tgcgcttccc gcggaagccg gcggccgaga acgcccggct tctgccaaaa aactttctct
    20101 cgccgggcgt tgggcaaagt ctgataatga ggacaaaatg cggtcgtatc gattgcatct
    20161 gccgctggcc aaagcggtag cagcggccgc cgcctcttgg gttgcggcct tccttctgca
    20221 cgcctatatc cttggcctgc cggccgccca cactacccga aatgtctgtt ttgggtgctt
    20281 tgcggggctc gcgtacgcac acctgttcga atatttcagc catgaagtgg ccatgcatcg
    20341 agggctggcg gggctgcgtt tcatccgcga tggccatctc cggcaccacc gcatcttcta
    20401 tggccaaaat tttcggtccc gccgcccgga ggatctccag catgtcacca ctgagtggta
    20461 cgcctttccc gtcctgatgg cgccgcacta cgcgctgctc gcggcgatct accccgccgc
    20521 tgcgccggcg ttctttttcg gcgtaaccgc gcacttcatc atttacgaga cgggccactg
    20581 gtttactcac gtggcggaaa acggctttga cagaacgctg gcctgcattc ccgggctggg
    20641 cgctcttcgc gcgcgccagg ttttgcatca tcaactccat cacgcccgcc cttcggtgaa
    20701 cttcaatttc accccgccgt acggcggtga tcgcctggct aaaacctacc gaaaagcgta
    20761 aggttcgcag accatcccgc gcacttcccc gcccgaataa cgccgttcgg ggcaacttcc
    20821 cgcgcaactg gctgcaatgg cttgctcagc cgggagctat gtgattcaat acaaaagaca
    20881 atgacacgga aaagtctgcg gcgtaagctc ttggctgcgc accggctgag ccgcgagttg
    20941 tggatgatcg cgaggggcct cgcgtccaag cggcatccga tcctcgccca catcataccc
    21001 attcgccgct gcaacctttc gtgccgctac tgcaacgaat acgacgactt ctccaagcct
    21061 gtcccggtgg aagagatgtt ccggcgcatt gaccgcttgg cgttgtttgg cacgacggtc
    21121 atcacgatca gcggcggcga gccgctgctg catcccgacc tggatagcat catccggcgg
    21181 atccggagcc gcggcatgat cgccggcatg atcacgaatg gttatttgct gaccgcagag
    21241 cgcatccagc gcctgaataa ggctggtctc gagcatttgc agatcagcat tgacaacctc
    21301 acgccggatg aagtttccaa gaagagcttg aaggtcctcg acaagaagct tcaacttctc
    21361 gccgagcacg ctgattttca cgtgaacatc aactcagtga ttggcggcgg gattcgcaac
    21421 ccggaagacg cgctcgtggt ggcgcgccgc gcgctcgaat taggtttcac cagcacggtc
    21481 ggcatcatcc atgacggttg gggccaggtg cgcgctttga gcgaacaaga gcagcggatt
    21541 taccgcacga tcaaggaatt agggaagcgc agctacgcca gggtcaatta ttttcaggag
    21601 aatatcgccc acggacgtcc caataattgg agatgccggg ccggcgggcg ctacctgtat
    21661 atttgcgagg acgggctggt ccactactgc tcacaacaac gcggctatcc cgcaacgccg
    21721 ctcgagcgct acacgccgca agaccttgag cgggagtact acaccaagaa atcctgcgct
    21781 ccttactgca ctgtttcctg cgtccaacag gtcgccacga tcgatttttg gcgcgatccc
    21841 caacatatcg aaccttccag atccgtttcc gagccgttgg tgcagatctc tacggccgcc
    21901 ggggaatgat cggaaggcag atcgagacgg ctaaggtttt cctgcgcaag taatacatag
    21961 aaaaaagggg gcgttccggt cagacgccgg tccccggcga tccggcccca agttatagta
    22021 gctgcatcgc cggctggccc gttccgtaaa gcgctgcggc cggcgcgcaa gcttatgtcc
    22081 gagctccgcc aggccgttct gccgcgcatg ctgctggcgt tattcttggt cctgtccggg
    22141 cgacacggcg tttcgggtca agcgccgcaa gccggccaag ggcagcgcgc acgccgggcc
    22201 gccggtccct gctctcccgt cgatccagtc tacgagcgga tcgcgaaggc gaccggcggt
    22261 caagttctca ggttgcgcgg cgccgatctg gcaaacaacc cgaatctcct tgccgctacg
    22321 ggcaacaaat cagccacgat tgtgtccgcc agcggccttc tccggccatc ggaatcgcgg
    22381 gagtttttgg ccccggtcga ttccactgtc gaagacatga ccttctcgat attcgtggag
    22441 tgcaaggggg agattcaggc cttccgcgcc tccggtgctg cggcgtcgga ggggccgggc
    22501 atcacaatcg ccaatgtttc ttcgggagaa gttttggcga tcagtcgtcc tgaaacaggg
    22561 gtctggcgcg tccgaacaag cggctcgggg tctttctccg tattagtcgg cgtggatagc
    22621 ccgattcaac tgcatcgatt tgagtttgtg aaacgcggcg ggcgtcccgg ccacgaggct
    22681 ttatttccca tcagcggcca gcccgtcctc ggggaaacgc agatcggcct cgcggggctc
    22741 tccggcccga cgggcgcagc cgcatttgag gtgcttgcgg aaagcggcga gcgattacaa
    22801 atgctgagtc tggctcaggg cagccccgat gccgccgctg atgaatttgt tgggcaagtt
    22861 tctcttcccg cgcgtccctt ccggattgcc atcaggggcg tggacccgcg gggctttcct
    22921 taccagcccg tttacccgcc gctgtttcat ccgcagccgg tggaggtgac cttcgtggcg
    22981 gccagcggcg agccagctcc cggcgccagg gccatggtcg attttgtggt gcgcaacgta
    23041 ggccggccgg cggtttttcg catcgcggcc gccgttggga ccggcgccat tcgcaatggc
    23101 gttccgcaaa ccttgtcatt gaacaccgga gaaacgggga tgctcacgtg ccggttaccg
    23161 taccgcccgg cgcggctgag ggatcgacga ttccagtgac cctcacggcc tcaagcccgg
    23221 tggatcccgc ggtttctaat agcgcgacgg cgcttctgac cgttagcccg gagcgatctc
    23281 gttaggcata gccatgtttc actcggtcca gcaccgcaat tctcacctga ttttatggcc
    23341 cgcactctga tcgaacgccg aaaaagcccg cggtatctct gttctcacat cgtcgagatc
    23401 tggcacggcc gcgagactac cgccggcctg ctggaggacc tgagcgccga gggcgccgcc
    23461 atcgcttttg aagccccgct cgaccgggac gagctcgtcg tgctggtagc cggaggactg
    23521 caagccagcg cccgggttcg ttactgcacg cggcgggaga acgattatcg cttgggtctg
    23581 gaattcacca gcggctcccg atggcagccg caccaatggc ggccggatca tctgttccta
    23641 cggccttcta acgaataatt tgtcccgccg ggttcgcggc cggggaaatc agatgcgctt
    23701 ccgccctaag agcgattttc gggcgccgct gaatctcttt ctacatcgcc cctccaacgc
    23761 cgcaggtgat ccaggcctgc atcgcattct cccaagaaaa gccccgccgg catgccagac
    23821 ggctattata atttggggca gcgggaaggc agatgaagca atacgatctg gtggtgatcg
    23881 gttcgggacc cgccgggcat cacggggcca ttcaggcagc caaactcggc aagaaggtgg
    23941 cagtagtcga ggcgaaatcc tgcgtcggcg gcgcctgcat caacacggga acaattccga
    24001 gcaagaccct gcgcgaggcg gtcttgcatc tctccggatt taagcagcga gggctctacg
    24061 gcggcgcatt cgacggcgca gccaacattt cgatgtccga cctgttgcag cgcgtgcagg
    24121 aagtgatccg cacggaaatc ggcgtattca atcatcagtt tcgccggaac ggcgtcgaaa
    24181 tcctgaacgg ccgggcctcg ttcgtcgatc cacacacaat ccgcgtagag ggcgtggatg
    24241 gcgaggtgca gtgccagggc caatacatcc tgatcgcgac aggtacacgc ccggcgcaca
    24301 atgattccat cgctctcgac ggatcggtca tttttgacac cgacgctttg gtccacttgc
    24361 ctgtcctgcc taaactgctg accattgtgg gcggcggcgt gatcggagtt gagtacgcct
    24421 gcatggccgc cgccctgggg atctcggtga cgctggtcga acggcggccg cgaatcctcg
    24481 atttcgtaga cgatcaaatc gttgaggcgc taagttacca catgcgcagc cgcggagtga
    24541 cgttccggct gggcgaagag gtcgcggagg tcatcaaaac gccggaaggc aaggtctgcg
    24601 cgaagctcaa gagcaagaaa caaatatggt ctgactcgct tctttacgct gtgggccgcc
    24661 aaggcaacac cgattcattg aacctggccg ccgccggcct ggaggcggat gcccgcggcc
    24721 gtcttccggt gaaggaaaat tttcagaccg cggtccctca catttacgca gccggcgacg
    24781 tgatcggctt tccgagcctg gcttccgtct cgatggaaca gggccgcctt gccgcgtgtc
    24841 atgcgttcca tcatccagct tccgcagttc cggaattgtt cccgtatgga atttattcca
    24901 ttcctgagat ctcctttgtg ggcgcgacag aaagccggct cacggaaaac gatgtcccct
    24961 acgaagtggg ggtcgccaac taccgggaga ttgccagggg ccagatcatc ggcgacacgc
    25021 acggtatgct caagctgctg tttcaccgcg aaacacgcaa actgctcggc gtccacatca
    25081 tcggcgaagg agcgaccgaa ttgattcaca tcggccaggc ggcgctctcc ttcggcggga
    25141 caattgattt cttcgtcaac accgttttta attacccgac tctggcggaa tgctacaaag
    25201 tggccgccct ggcggggctg aatcgcctca actattccgg ctcgctctga caatcgcaga
    25261 gcagttgaaa accatcgatg cggcgatacg gtatgatggc gtgattgcag cagtgtaatt
    25321 ttcccgggag gagcggtact tactttgagc ggaaaaacgg cagtcgctgg ctatacgcac
    25381 gatgaagtcg aggaactgct ggcccaaggc aatccaggcg gaaggctgac gaagcacgac
    25441 ctcgcgacgc ccgccctgat ccttgatctc gaccttttcg aaagcaatct ccaaaaaatg
    25501 gcggcgttcg tgcggtcgcg cggccgcggc ttccgcccgc acgccaaaac gcacaaatgt
    25561 cccgaaatcg cccggcgcca gcttgcagca ggggccagcg gcgtggccgt cgctaagatc
    25621 agcgaggccg aagtattcgc gaaagagggc attggcggtt tactggtcac aacggcaatc
    25681 gtcggcgcgc gcaaaatcgc gcgggccgtg cgcctggcgg cggcccggcc ggagacgact
    25741 tttgtcatcg ataacgagac caatgcccgc gagctttccg aagcggccca agccgaaggc
    25801 atccgtatca aggtagcgct ggatctctat gtgggccgcc gaaccggcat ccttccaggc
    25861 cgtccggccc tagacctcgc ccggcaggtc gccgccatgc gcgggctcga tctggagggc
    25921 attcaggcct atgccgggca agcgtctcac gtcatcggat tcgaggagcg ccgccgcttt
    25981 tcgcgcgagt gcatgagcca ggccgttgaa acccgctgtt tgctggagca agcggggatt
    26041 gaggtccgta tgctcgccgg gggtagtaca ggcacttaca atattgatat agagttggac
    26101 ggcatcaccg aacttcagcc gggaagcttt atcttcatgg acgtggatta cgggcgcatc
    26161 ggcggccctg actccgacgc ctacacggat ttcgccaagg ctctgacggt cctcgcgaca
    26221 gtggtcagcc gccccgaatc ctgcaaggca attgtggatg ccggattgaa ggcgttttcc
    26281 acggacaagc cattcccgcc ggaatccaag gacctccccg gcctgcttta cggctttgcc
    26341 ggcgacgagc acgggcggct cgatttctcg cacgccggat gcgagctaaa agtgggagac
    26401 aggctcgaat ttgtcattcc gcactgtgat cccactgtca atctgtacga caggttgtat
    26461 tgcgtccggg gggagaaggt ggaagaggtc tggcggattt ccgctcgcgg gatgagccag
    26521 tagcatacat aaccagaccg tgcctttcgt ttctgcacgc ggcagaaaat aagatcaggc
    26581 ggcgccccgc gaaacagctc gcgcccggcg gggcgcggct cgccccagta acaggtccaa
    26641 aaaggagggt gactccggaa cctccgcggg ctgcggcggc agatcgcatc cggccagagt
    26701 gcaggcaaat tcgcggatgg cgctcccgag attcgaagcc gcatccacca cagacccgga
    26761 acgcaacgct ctgcgaacgc tcgtgtcatc ctccggcagg acggcggtga tgcggcgctc
    26821 caaaaacttt tccatttcgg ccaacggagg atcgtcttcc ctgtgctggt tcacggccac
    26881 gctgatccgg gtgcgcgaaa cgccgatgcc ccgcagctcc aaaactttcc tccttgcgag
    26941 aaagagggag gccgtttcgg gcgtggcgac gatgtaaaaa tccttggcct gcgtcacgac
    27001 cgcttcggtc aactccgttg cgccttccgg cagatcgaca atcaccgcgt catactgcga
    27061 ccgtacgaac cggaagaacc ggtgatagct cagcttggaa aaggccgtgg ggctcgggat
    27121 gcgcggtgcg cccagcacgt cgatgccgcc ggcgcttgac cgcagttgca tccaatgatc
    27181 ctgatccagt tggttcgata tctccagggc gtcggttacg cttctctccg gattcaggtt
    27241 caggagaagg caaaccacac ccgctcgcag atcgccgtca accagcagga tcttcttttt
    27301 ccagtcccga gccagcgcgc cggcaacgtt caacgcggtg gtcgtcgctc cgctgccggc
    27361 tttggccggc acaaacgcaa acaggttttc cgggggaccg gccttgccgg agtccagcac
    27421 ctgaatgatg gcttggtgaa aatcgtcttc atcgaagggc ggaatcagta tctctgagat
    27481 tcccgcctcg agggcctcga gcgccttttc acggccgcat cgctgtgcga agccgataat
    27541 caccgtctcg gggcttgagt tgcggacctc ccgggccaga tcgaaggcgg tttgcgcggc
    27601 ttcaatttcg aggaaaataa tcttcggcgt gaaggcgttc aggaatcgga cgatctcgta
    27661 gatggaggga taggcgtaaa gcgtcttgta gacgcaaaga ttctcaaccg acgcgcaccc
    27721 ccgctggatg ccgaacacca gatgccggga tggcccgaaa atgagggcgg acaacataaa
    27781 agggggataa acatctacct ctcaggaatt ctaaatcggc cgtaccatgc gcccaaccag
    27841 tcgatcttct gagaccgcct gccatttcta cttactactg ctcgaaaatc tgctaagccg
    27901 aacgtcatac gccgccaaaa cagcgccgcg cgactgcgtc tttcctcttg ggtttcccag
    27961 tagcgcaggc ggggccgctt gccgcaatcc tatttgtatc ctccgcaaaa aaggcacgat
    28021 ccaccccttg ggcgtacttc cctatggggt agaaagattt tcggaagacc cggcgctcag
    28081 gcgccggcac gatccacaaa tttctttaag atattccgaa cttcggaagg tggtggatca
    28141 tcgggccgcc gctcggattg cagatagcgt gctagaagct cgcgggcccg ctggggatcc
    28201 cggccggaga tcccgagttc ctttccccaa gcgaacagca acccaggaga attcggatcg
    28261 gcgctcgcag cgcggcagaa cagcgcatcg gcttcggcat gtctcccccg gcgggccaag
    28321 aacgaagcga gatcgagcag acgcccggtg tttcgcggtt gcaggtcgag cgcccggcga
    28381 aaccccgcct ccgcccgggc gtagtccctg cgcttgacgg caatcagccc gtcggcccgg
    28441 cttccttccg ccgcgtcgat ggccccgagc cgggacgcga tggcgctcgc tttttcctcg
    28501 ccgccgccta aagcggaggg ggcttccaga taataatcca gcaggtcgtt cagcgcgccg
    28561 acattagttc tgtcgagctc gaccgccttt tcaaaggcca ggcgagcttt ccgggcgagg
    28621 cccggagctt tcaagatgtt ggagcgctcc gctctgcgtc ctgcggcttt ccccagccac
    28681 tgatagtatt cggaaacagc gggttcggca gctatggctt gctccaacag cctgatggcg
    28741 ccctcgtaat cctcgaccgc gtatgcctga acgccctgtg aaaataacgc gcgaggggat
    28801 tgcacccctc ggagaggctg agagagaccg gccgcgcaga gcaggagcca gatggcaaac
    28861 gccaggcgcg cacgcccgcg cagcaggtac aggccgcgat gtgttgcccg gttcatatca
    28921 aaaaactgaa tcgccgctct tgcccttcta ctgactctga tgcttagcga gtaaattaag
    28981 tggatgcggt ttgcgccaaa cgcgattctt cccgcgccat cgacccgtcc aaaatcgtac
    29041 aagtttggta cgctaaactc gccccattcc gcacagcatc ccgactggtt caatcaacgt
    29101 tggactatcg ccggcaacgg cgggttccaa agaaaaacgt ggccgctcag aacggccgca
    29161 cggcaggtaa taacagacag agcagggatc agccgaagtg agtactagga ggatccctag
    29221 caggtaatgg aagaagcaaa cattgtgaca agtccgggcc gcctttggga cacacccagc
    29281 agcccggcga caagtggaat catgctaagt ttatgcgagg aaagcaacgc gcccgtagct
    29341 cagttggata gagcggcaga cttcgaatct gtaggtc
//
LOCUS       DASREK010000296        11491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1624457, whole genome shotgun sequence.
ACCESSION   DASREK010000296 DASREK010000000
VERSION     DASREK010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624457"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFA54_15975"
     CDS             <1..273
                     /locus_tag="VFA54_15975"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HZS52368.1"
                     /translation="GTDASKSPENTGIEQAAKNREIVKAIHAINAKEAFGPGSELRFA
                     VDRDTGRPLIRIVDRATNEVLNQIPPEDVLRLAEVLGQLQGRARMA"
     gene            335..751
                     /gene="fliS"
                     /locus_tag="VFA54_15980"
     CDS             335..751
                     /gene="fliS"
                     /locus_tag="VFA54_15980"
                     /inference="COORDINATES: protein motif:HMM:NF014604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZS52369.1"
                     /translation="MHGRYLENEVLSADPIGLVQLLYAGAIDAIRKARVFLQEGDIHG
                     RSATIAKAMQIILELQSSLDLERGGEIAVNLARLYVYIQERLAEANARQALAPLDEVL
                     QLLLILNEGWEQAARQSMAAPPAEALGAAGPANWTL"
     gene            827..2881
                     /gene="fliD"
                     /locus_tag="VFA54_15985"
     CDS             827..2881
                     /gene="fliD"
                     /locus_tag="VFA54_15985"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZS52370.1"
                     /translation="MASSPVSPSTSALNLTQPLTFTGVSSYSSDLQQILQRAVQIATL
                     PMQKLQLDQASILSQEQALSSLGGAVSRLTSSFSSLGLLGANGALTATTSDPAVATVQ
                     LTGSPAPLTYSLVVTSAAAAAQASTALGLPDSDTSAPRANGLYTLTLGSNNQSFDLRA
                     TGSGRTAGTIGDALPSEPDSVTITFANGLTGSITAGLKSFFVGNSPVGGAAAGDSVSV
                     NFTSDDGAINTGITATLAGGEDAAALANLLNQQIAGNASLTGKISFSAVNGRLKLVES
                     DTAGTGFTFTSSTTGAITTGLEPGGAIGGESAQEIAAALNAQVDSNPALSAAGVRFSA
                     SQGEIQVTAAAGRQFTFTATDSSQGTGFASGLAGRTRVVGYSNTLSGLRDYINSQEAA
                     LGVRATVINTSSDPAHPRYDLTLTADQTGAQTLTLNDSTGSDLLPNGGMLGADAVFSI
                     NGGPAITNTSNTITGLVPGLNLTITGPSGATPVTLSVQTDRSAVESALQDFAVKYNAV
                     VDQLSSQIGKNAGPLGGSSIVRQIGGALHAITGFSSTAGSIQSMAALGLLLDRQGHLS
                     LDLPTFNSLTDQQFQDAIRFLGDTTAGFAGNAYTLLNQLTDPVSGAIQNTQNFLNESD
                     QRLSLQIAAQQDYVSRVQSTLTEQISQADTLLAQLESQQTLLTTLFKDQQAIALANTL
                     GG"
     gene            2883..3236
                     /locus_tag="VFA54_15990"
     CDS             2883..3236
                     /locus_tag="VFA54_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52371.1"
                     /translation="MPCQNLDPEDLLRALHASTDELCRAVDAGDLAGAGVAAARRGQD
                     ADALARCPQIWSPDLLKRLEKVLSHGEAAARALAGRRETARALLSEIEPVRLRLAKSA
                     PVQNTCSPQKLDLKA"
     gene            complement(3290..3532)
                     /locus_tag="VFA54_15995"
     CDS             complement(3290..3532)
                     /locus_tag="VFA54_15995"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HZS52372.1"
                     /translation="MLVFRRQQGESFMVGDQVEVRILEISRAQVKIGVIAPREVGIYR
                     SEISRLNRSAVLGDLRKPEVKAAIAAVRKALSKNDG"
     gene            complement(3532..3939)
                     /locus_tag="VFA54_16000"
     CDS             complement(3532..3939)
                     /locus_tag="VFA54_16000"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HZS52373.1"
                     /translation="MAQPQPSPTGVIHFPDGLPGFESLRQFVLLQEEELLPIVFLVSL
                     IEPKICLPLAPIHHIRSDYQLRLTEDDRRMLELPAEVIPGLNVLCMAVLNLGDGTQKP
                     SANLLAPIVINPANCTAKQIIQIDSPYSTVTEV"
     gene            complement(3973..5592)
                     /locus_tag="VFA54_16005"
     CDS             complement(3973..5592)
                     /locus_tag="VFA54_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52374.1"
                     /translation="MPFLKRHILSLALLAQAGLLCFHIDLLPIWGDERYTLETARLPP
                     ARILEALRVDVHPPLYYFLVKGWLKLPLPGAELARARELSLLFALLATVAVYRLWLAN
                     IRFRDRCLFLGLWVGSPFLLLYARMARSYTLQLLLAVIAIRTARDWLRSPRNARATAR
                     YIFAAVALLYTHYLPGLAVVTGTALVSLCRRQWRQLLALPAIALAYAPWLRTLIETSG
                     MVARGQPYQVSGNVLIAAAVRVAYTFTAFNFGESIPPWGILLAAGVSPALLWALWKAW
                     RRTDRVPLLFLAVAGIAYFGASAWVTFAFLGARLLFLLPFYYLFLLRGLDTRRIAGLA
                     AYAALVIVACGGLGSYYRKRDFLNKGYLVDYAAIAGRVAARSRGEPALVLLDSHVSDA
                     GYALHEAGFRGRVEILNDAKNFDQALAEIRHHHPSLVWYIRYGRDLTPAGLHHHLEAE
                     VSREYTVERHGAVPFSWIDRKAMQWLRMAEQPAYLVEVLEMRPRGPQERGKRSLERGY
                     PSTGAWKGPLHLISNAPKSPPGPCGCEPGEI"
     gene            complement(5624..6469)
                     /locus_tag="VFA54_16010"
     CDS             complement(5624..6469)
                     /locus_tag="VFA54_16010"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52375.1"
                     /translation="MSLGKVLRLRRLLRKGRALVVPLDHGLVAGPLKGIEDPVGLIKL
                     IAQSDADAVLLTPGVLDQVAGELGQLAVVLRIDGCTSVQGSGPMRQFASVAHAAALGA
                     DAVIVNATVGGGQEAAELRKLGRVAEEARRWGMPVIAEMLSARMLANHLDFTGRGGAA
                     LPPDVAGDIALAARIGAEMGADIIKTRYPGEEASFREIVAATGRPVMVAGGPMRDSSL
                     RSLYQLVDQSLSAGAEGVIFGRNVWQHARPDAVLAGLAAMVHDAATVEQALAVADEQP
                     CPAVP"
     gene            6533..7495
                     /locus_tag="VFA54_16015"
     CDS             6533..7495
                     /locus_tag="VFA54_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZS52376.1"
                     /translation="MKERFLAAAAQISPVYMQLGPTVEKVCQTIREVGSQGAELVVFP
                     EVVIPGYPYWRGLYPVPEWTAWMVRYQLNSLVIPSNYTDRLCEAAREARCFCVIGCSE
                     RSGWAGSETLYNTALFINGEGVIVGKHRKLMPTHGERMVWGMGDGSDLQVFETSLGRI
                     GALICYEHHMTLLKAAMAALGEEIHCALWAGYWVMESHPGAKRRWRAGDSTDLCEVAY
                     ANREYAFETQNFVVSAGNYDPAGSLPSECSAWDIGSGGSAILNPAGLYLAGPALDQEA
                     ILWAELRAEDRQKTKAYIDTLGHYSRFDVVRLCMNKSKLTPAGP"
     gene            7582..8556
                     /locus_tag="VFA54_16020"
     CDS             7582..8556
                     /locus_tag="VFA54_16020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZS52377.1"
                     /translation="MRYRRLGRTGLDVSEIGYGAWGIGGMMWVGAEDQESLRALHRAI
                     DLGVNFIDTALVYGDGHSEKLVGEVVRYRSEPIYVATKVPPKNMLWPARPGISIDQVF
                     PYWHILQATEESLRHLRLERVDLQQLHVWSPEFTASEEWRRAVEDLKKSGKVRYFGIS
                     ANDYQPASLLDALRTGLIDCVQVIYNIYDPRAADALFPLCQQLGVGVIARVPLDEGGL
                     TGRITPETTFPNGDFRNRYFRGERRRQVWEHARKLQQAVGEAHGTLAQVALRFCLSHP
                     AVSTVIPGMRSVPNVESSCAVPDMGPLPADILEELRRHIWIRDFYSAA"
     gene            complement(8581..9291)
                     /locus_tag="VFA54_16025"
     CDS             complement(8581..9291)
                     /locus_tag="VFA54_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZS52378.1"
                     /translation="MRKSDAFRDLQNLQEQVIQCRRCPRLVEYREVVAKTKRRAYLDW
                     EYWGRPVPSFGDPAARVLVIGLAPAAHGANRTGRMFTGDSSGNFLYRVLHRAGFASQP
                     TSVSRDDGLRLKDLYITAAIRCCPPGNKPLPQEFANCRPYLERELDLLPNVAVVVTLG
                     KLAHDTYLTILKSRGAISRRSAYPFRHGAVYKFGQGLPTLMASYHPSQQNTQTGKLLE
                     PMFLRIFEAAKKAAAARS"
     gene            9546..10241
                     /locus_tag="VFA54_16030"
     CDS             9546..10241
                     /locus_tag="VFA54_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52379.1"
                     /translation="MKKQARTARERKAGRPSSADRKGLSPARRPESGRRKPIALPPNN
                     KASATAALVSARVRPGGQQRDFTAEQQQKAYDEAMRLFQAHKFERADELFRKATEGPN
                     RTLAHHAQIHSQICQKRLAPPEPKLKTADDHYNYAVTMMNARRLKEAAEHLEMALHMA
                     PQADHLYYALAATEALQGNPQGAYERLKTAIHLQPRNRILARGDADFAGILEYPPLAS
                     LLQLDRGWSPKAS"
     gene            10280..>11491
                     /gene="glmU"
                     /locus_tag="VFA54_16035"
     CDS             10280..>11491
                     /gene="glmU"
                     /locus_tag="VFA54_16035"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZS52380.1"
                     /translation="MNHDFAIVILAAGLGTRMKSRQAKVLHRAGGKTLIELVTSTAVR
                     LVPPERVYVVIGHQGGLVRQVLDGSGVRFVEQTEQLGTGHAIASGRGCLDASGKRLLV
                     LYGDCPLVTPGTLEALMRRHVESGAAATVLTTTLENPSGYGRILRGSSGAVQAIVEEK
                     AATPEQKSIREINSGMYCFETAPLFEALAALRPDNPAKEYYLTDVIAGMVGRGLKVEA
                     CHTAESSEVLGINTRVELAAVDAVLRSREVRRLMLEGVTIQKPETVIIDAGVSVGEDT
                     VVEPFVQVLGRCRIAANCWIRAHSIISDSVLGEGVVVEPFSIIQESEVQRGARIGPFA
                     RLRAGNIVGAEARVGNFVELKKTAFGARSKAMHLAYLGDAAVGADVNVGAGTITCNYD
                     GTAKHPTVIDDG"
BASE COUNT         2315 a         3577 c         3558 g         2041 t
ORIGIN      
        1 ggaaccgacg cctcaaaaag cccggagaac acggggatcg agcaggctgc gaagaatcgc
       61 gaaatcgtca aggctattca cgccatcaat gccaaggaag cgttcgggcc gggcagtgag
      121 ctgcggttcg ccgtggatcg cgataccggc cggccgctga ttcgaatcgt ggatcgcgcc
      181 accaatgagg tgttgaacca gattccaccg gaggacgtgc tccggctggc cgaggtgctc
      241 ggccaattgc aagggcgcgc cagaatggcg taaatccagc ctggcgcaaa gccggcggcg
      301 aacggcgatg ggcccccagg ggagaaatag agtcatgcac ggccgctatc tggaaaatga
      361 ggtcctttcg gctgatccga tcgggctggt gcaactacta tacgcaggcg cgatcgatgc
      421 gatccgcaag gcccgagtat ttttgcagga gggtgacatc cacgggcgct ccgcaacaat
      481 cgcgaaggcg atgcagatca ttctggaact gcaaagctcg ctggatctgg agcgcggtgg
      541 tgaaatcgcg gtcaacctgg ctcggttata tgtctacatt caggaacggc tggccgaggc
      601 gaacgcccgg caggcgctcg cgcccctgga cgaagtgttg cagttgctgc tcattctcaa
      661 cgagggttgg gagcaggccg cgcggcaaag catggccgcc ccgccggcgg aagcactggg
      721 cgccgccggc ccggcaaact ggaccctgta gggccgcatg acccgctgcc cgcccggctg
      781 aaactccggt tcgagtttcg aatgcaaaac gcatcgagga agtatcatgg ctagctcacc
      841 ggtttcccca agcacgtccg ctctgaacct cacacaaccg ctcacgttca ccggcgtaag
      901 ctcgtactcg agcgatttgc agcagatttt acagcgcgcc gtgcagattg cgacgctgcc
      961 catgcagaag ctgcaattgg atcaggccag catactcagc caggaacagg cattgagtag
     1021 cttgggcggc gctgtcagca gactcacgag cagcttctcg tcgctcggct tgctgggggc
     1081 caacggcgct ctaaccgcca cgacatccga ccctgcggtg gcgacggtgc aattgacggg
     1141 atcgccggcc cctctgacct actccctggt ggtcacttcg gccgcggcag ccgcccaggc
     1201 gtctacagcc ctcggtctcc ccgacagcga tacatcggcg ccgcgcgcca acggcctgta
     1261 tacgctcacg ctgggaagca acaatcagag cttcgatctc cgggccaccg gctccggccg
     1321 caccgccgga acgattggcg acgcgctgcc ctcggaaccg gacagcgtaa cgatcacctt
     1381 cgccaacggc ctcacgggca gcatcaccgc cggcctgaaa agcttctttg tgggaaattc
     1441 acctgtcggg ggcgccgcgg caggcgacag tgtcagcgtg aatttcacga gcgacgacgg
     1501 cgccatcaac actggaatca cggccacgtt ggccggagga gaagacgcgg ccgcactcgc
     1561 caacttgctc aatcagcaga tcgcgggaaa tgcgagcctg accgggaaaa tcagcttcag
     1621 tgcggtgaac ggccgtttga agctggtgga atccgatacg gcaggtacgg gcttcacctt
     1681 cacatccagc actaccggcg cgataaccac gggcttggaa ccgggaggcg cgatcggcgg
     1741 tgaatcggcc caggagatcg ccgccgcttt gaacgcgcag gtcgattcga atcccgctct
     1801 ctcggcggcc ggcgtgaggt tctccgcctc ccagggcgag atccaggtta cggcggcggc
     1861 cggccggcag ttcacgttta cggccaccga cagctcccaa ggaaccggct tcgcttcggg
     1921 acttgcgggc agaacacgag tcgtcggcta ctcgaacacg ctttccgggc tgcgggacta
     1981 catcaattct caggaagcgg cgctgggtgt tcgcgccacc gtgatcaata cgagctcgga
     2041 tccggctcat cccagatacg atctgacgct cacggcggac cagaccggcg cacaaacgct
     2101 gactctgaat gacagcacgg gctccgatct tctgcccaat ggaggcatgc tcggcgctga
     2161 cgccgtgttc agcatcaacg gcggcccagc catcaccaac acgtcaaaca ccatcaccgg
     2221 cctggtcccc ggcctcaacc tgacgattac cggacccagc ggggccacgc cagtcaccct
     2281 cagcgtgcag actgatcgca gcgccgtcga gagcgcgctg caagattttg cggtcaagta
     2341 caacgcagtc gtcgatcagt tgagctccca aatcggcaaa aacgcggggc cgctcggcgg
     2401 ctccagcatc gtgcgccaga tcggcggcgc gctgcacgcc atcaccggat tcagttctac
     2461 ggctggctcg attcagtcca tggcggctct ggggctgctt ctggaccgcc agggacattt
     2521 gtcgctcgac ctgccgacct tcaacagcct caccgatcag caattccagg atgccattcg
     2581 cttcctgggc gatacgacgg cggggttcgc cggcaacgcc tacacgcttc taaaccagtt
     2641 gacggacccc gtaagcggag ctattcaaaa cacgcagaat tttctcaacg aatccgatca
     2701 gcggctttct cttcagatcg ccgcgcaaca ggattacgtc agccgggtgc agagtacgct
     2761 taccgagcaa atttcccagg cggacacctt gctcgcccaa ctggaatcgc aacaaaccct
     2821 gctcacgacg ctgttcaagg atcagcaggc catagcgctc gccaatactc taggtgggta
     2881 gaatgccgtg ccagaatctc gaccctgaag atctactgcg cgcattgcac gccagcacag
     2941 atgaattgtg ccgggccgtg gatgccggcg atctggctgg cgccggcgtg gcggcggcga
     3001 ggcgcggaca agatgcggac gctctcgcgc gctgcccgca aatctggtct ccggatctcc
     3061 tgaagcgctt ggaaaaagtc ctgtctcacg gcgaggcggc cgcgcgggcc ttggcaggcc
     3121 ggcgcgaaac ggcgcgagcg ctgctcagcg aaatcgagcc cgtgcgcctg cgtctggcga
     3181 agtccgcgcc ggtccaaaat acgtgttccc cccaaaagct cgatttgaaa gcctgacttc
     3241 gcagccgccg tctccatcgt tttgcggcgt ccacgcgggc ggggcgacct caaccatcgt
     3301 ttttggaaag cgccttccgc acagccgcga tggcggcctt cacctccggc tttctcaaat
     3361 cacccagaac cgcgctgcga ttcaggcggg aaatttcact ccggtagatg cccacttcgc
     3421 ggggcgcgat gacgccgatc ttcacctgcg cgcggctgat ttccaatatc cgcacctcga
     3481 cctgatcccc caccatgaac gattctcctt gctgccggcg aaaaacgagc atcacacctc
     3541 ggtaaccgtt gagtacgggc tgtcgatttg gatgatttgc ttagccgtgc agtttgccgg
     3601 attgatcacg atgggcgcca gaaggttggc ggaaggcttt tgcgttccgt ctcccaggtt
     3661 cagcaccgcc atgcagagaa cgttcaatcc cggtatgacc tcggccggca gctcaagcat
     3721 gcggcgatcg tcttccgtca ggcggagctg gtagtcggat cgaatatgat gaatcggcgc
     3781 caggggcagg cagattttcg gttcaattaa ggagaccaga aacacgatcg gcagcagctc
     3841 ttcttcttgc agcaacacga attgccgcaa actctcgaac cccggcaggc cgtccgggaa
     3901 atgaatgaca ccggtagggg acggttgcgg ctgtgccatc aaaacctcta tcggcaaggg
     3961 gcggccccgg ggctatatct cccctggttc gcagccgcac ggaccaggcg gcgacttggg
     4021 agcgtttgag atcaggtgaa ggggcccttt ccacgcgccc gtgcttggat agccgcgctc
     4081 cagtgagcgt tttccgcgtt cttgcgggcc gcgcgggcgc atttcgagaa cctccaccag
     4141 gtaggcgggc tgctctgcca tgcgcagcca ctgcatggcc ttgcggtcaa tccaggagaa
     4201 tggaaccgcg ccgtggcgct cgacggtata ttcccgcgaa acttcggctt ccagatgatg
     4261 gtggaggccg gctggcgtca gatcgcggcc gtagcgtata taccaaacta gcgatggatg
     4321 gtgatggcgg atttcggcta gcgcctggtc aaaattcttc gcgtcattga gtatctcgac
     4381 acggccgcga aaaccggcct catgcaaagc gtagccggcg tcggaaacat ggctgtcgag
     4441 caataccagc gccggctcgc cccgggagcg tgccgccact ctaccggcga tggcggcgta
     4501 atcgaccaga taccccttat tgaggaaatc ccgtttgcgg tagtagctgc ccaggccgcc
     4561 gcaggccacg atgacgagag cggcgtaggc cgccagtccc gcgatgcggc gggtatccaa
     4621 gccgcgcagc aggaataggt agtagaaagg caagagaaac aacaggcgcg ctccaaggaa
     4681 ggcgaaggtc acccaggctg aagcgccgaa gtacgcgatg cccgccacgg ccagaaaaag
     4741 aagaggaact cgatccgtcc gccgccacgc tttccacaac gcccacagca aagcggggga
     4801 gaccccggct gccaggagga tgccccaggg cgggatactc tcgccgaagt taaaggccgt
     4861 gaacgtgtat gctacccgga cggcggcggc gatcaaaacg ttcccgctca cttgatacgg
     4921 ctgcccccgc gccaccatgc ccgaggtttc aatcagggtt cgcagccagg gagcgtacgc
     4981 gagcgcgatg gccggcagtg cgagcagttg cctccactgc cgccggcaca aactcacgag
     5041 cgccgtcccg gtcaccaccg ctaggcccgg caaataatgc gtatagagca gcgccacggc
     5101 ggcaaagatg tagcgtgccg tcgctcgggc attgcgcggc gaacgaagcc agtcgcgggc
     5161 cgtacgaatg gcgattaccg ccagcagcag ttgcaacgta taggagcgcg ccatccgggc
     5221 gtataagagc aggaagggag atcccaccca cagtcccaga aaaaggcagc ggtctctgaa
     5281 gcggatattc gccagccaaa gccggtagac cgccacagta gcaagcagcg cgaacagcaa
     5341 ggagagctcg cgcgcccgcg ccagttcggc accgggcagc ggcagcttca gccagccttt
     5401 gactagaaag taatagaggg gcggatgcac atccacccgg agcgcttcca gaatacgcgc
     5461 cggcgggagc cgggccgttt ccagcgtgta gcgctcgtca ccccagatcg gcagcagatc
     5521 gatgtgaaag cacagcaagc ctgcttgcgc caggagagcg agcgaaagaa tatgcctttt
     5581 caggaatggc aaggggcgcg aatggtctcc cgcttcctgc gagtcaggga acggccgggc
     5641 atggctgctc gtccgctacg gccagagcct gctccacggt ggcggcgtcg tggaccatgg
     5701 ccgcaaggcc ggccagcacg gcatcggggc gtgcgtgctg ccagacattg cggccgaaga
     5761 tgaccccttc ggcgcccgct gacaacgact gatcgaccaa ctggtagagc gaacgcaagc
     5821 tggagtcgcg catcgggccg ccggcgacca tcaccggacg cccggtggcg gcgacgattt
     5881 cccggaagct cgcctcttcc cccgggtatc ttgtcttaat aatatcagcc cccatttcgg
     5941 cgccgatgcg ggccgcaagc gcgatatcgc cggccacatc cggcggcaga gccgcgccgc
     6001 cccggccggt gaagtcgaga tggttcgcga gcattcgcgc gctcagcatc tccgcgataa
     6061 cgggcatgcc ccagcggcgc gcttcttcgg ccacccgccc gagtttgcgc agctcggccg
     6121 cttcctgccc gcctcctacg gtagcattca cgattacggc gtccgcgccc agcgccgccg
     6181 cgtgcgctac ggaagcgaat tgacgcatgg gcccgcttcc ctgcacgctg gtgcatccat
     6241 cgattcgcag caccacggcg agctggccca gttctccggc gacttgatcg agcacgcccg
     6301 gcgtgagcag cactgcatct gcgtcggact gcgcaatcag tttgatgagc ccgaccggat
     6361 cctcgatgcc cttcaggggc cccgcgacca atccgtgatc cagaggcacc accagcgccc
     6421 gccccttccg cagcagccgc cgcaatcgca aaaccttgcc caacgacata cgctctcctg
     6481 tctcgcattc tatggtactt ccaggaagcg gcatctgata gcatcaaaat tcatgaaaga
     6541 aagatttctg gcggcggccg cgcagatctc gcccgtttac atgcagctcg gccccacggt
     6601 ggaaaaggtt tgccaaacca ttcgcgaagt cggttcgcaa ggcgctgagc tggtggtatt
     6661 tcccgaggtg gtgatacccg gctacccgta ctggcgcggt ctttatcccg tccctgaatg
     6721 gaccgcctgg atggtccggt accagttgaa ttcactggta attcccagca actacacgga
     6781 ccggctgtgc gaggccgccc gcgaggcgcg ctgtttctgc gtcatcggct gttcggaacg
     6841 aagcggctgg gccggcagcg agacgctata caacacggcg cttttcatca acggcgaggg
     6901 cgtcatcgtc ggtaagcacc gcaaactgat gccaacgcac ggcgagcgga tggtctgggg
     6961 catgggcgac ggcagcgatc tgcaagtgtt cgaaacctcc ctgggacgca ttggggcgct
     7021 gatctgctat gagcatcaca tgacgctgct caaggcggcc atggcggcgc tgggggaaga
     7081 aatccattgt gctttgtggg ccgggtattg ggtgatggag agccatcccg gagccaagcg
     7141 gcgctggcgc gctggagact ccacagacct ttgcgaagtg gcctacgcca accgtgaata
     7201 cgcattcgag acgcagaatt tcgtcgtcag cgctggcaat tatgatccgg cgggttccct
     7261 gccgtcggaa tgctccgcct gggacatcgg ctcgggaggc agcgccattc tcaaccccgc
     7321 cggcctttat ctggcggggc cggcgctcga tcaggaagcg atcctttggg cggagctccg
     7381 cgccgaggac cgccagaaaa ccaaggccta catcgatact ctcggccact acagccggtt
     7441 tgacgtcgtc cgcttatgca tgaataagag caagctgacg ccagccggcc catagaagca
     7501 tcccatcgag tatcggagag cgttgattct cggtctcgat ctccggctgg cgtcccggaa
     7561 tgccgccgga ggagcttcat catgcgctat cgcaggctgg gaagaacagg actcgacgtc
     7621 agcgaaatcg gctacggcgc ctggggcatc ggcgggatga tgtgggtcgg cgccgaagac
     7681 caggagtcgc tgcgggcgct ccaccgcgct atcgatctcg gcgtgaactt catcgacacg
     7741 gcgctggtct atggcgacgg acacagcgaa aagctggtcg gcgaagtggt tcgctatcgt
     7801 tcggaaccca tttacgtggc taccaaggtt ccaccgaaga acatgctctg gcccgcgcgg
     7861 ccgggcatca gcatcgacca ggtgtttccc tactggcaca ttttgcaagc aaccgaggag
     7921 agcctccgcc atctgcggct ggaaagggtg gatctccagc agcttcatgt gtggtcgccg
     7981 gaattcacag cctccgagga atggcgcagg gcggttgagg atctcaagaa gagcggcaag
     8041 gtgcgttatt tcggcatttc cgccaatgat taccagcccg cctcactgct cgacgcgctg
     8101 cgcacggggc tgatcgactg cgtgcaggtc atctataaca tttacgatcc gcgtgcggcc
     8161 gatgccctgt ttccgctctg ccagcagctc ggcgtcgggg tcatcgcgcg ggttccactc
     8221 gatgagggcg gcctcaccgg ccggataact ccggaaacga catttcccaa cggcgacttt
     8281 cgcaaccggt attttcgcgg cgagcgccgg cgccaggttt gggagcatgc ccggaagctc
     8341 cagcaagccg tgggtgaagc acacggaact ctggcgcagg tggcgctccg gttttgtctg
     8401 tcgcatccgg cggtctccac cgtaattccc ggaatgcgga gcgtgccgaa cgtggaatcc
     8461 agttgcgctg tcccggacat gggcccgttg ccggcggata ttctggaaga actgcgccgc
     8521 catatctgga tccgcgattt ctacagcgcg gcgtgagcgg gcgcgatagg cggaacaggt
     8581 tcaggagcgc gcagcggccg cctttttcgc cgcctcgaaa atcctcagaa acatcggctc
     8641 aagcagcttg ccggtctgag tattctgctg actgggatgg taggaggcca tgagggtcgg
     8701 aagaccttgc ccaaatttgt acacggctcc gtggcgaaac ggatacgctg atctgcggga
     8761 aatggcgccg cgcgatttga gaatggtcag atacgtgtcg tgtgccagtt tgcctaacgt
     8821 gacgacgacg gccacgttgg gaagcagatc gagttctctt tccaaatagg gccggcaatt
     8881 ggcaaattcc tgcggcaacg gcttgttgcc cggcgggcag cagcgaatgg ctgcggtgat
     8941 gtaaaggtcc ttcaaacgca ggccgtcgtc tctggaaaca gaagtcggct gcgaggcaaa
     9001 accagcgcgg tggaggacgc ggtagagaaa attacccgag gagtctcccg tgaacatccg
     9061 cccggtgcgg ttggcgccgt gcgccgcggg cgcaagcccg atgaccaaca cgcgagcggc
     9121 cgggtctccg aaagacggaa cggggcgtcc ccagtactcc cagtccaggt aggcccgccg
     9181 tttcgtctta gcgacgactt cgcggtattc gaccagccgg gggcagcggc ggcattgaat
     9241 gacttgttcc tgaaggtttt gcaggtcgcg gaaggcatct gatttcctca tagaccgttg
     9301 atgttaccgc cttgggaagt aaagcggccg tcgctcgatc gttcgagccg ggtcttgccg
     9361 ccaagccacc aaccggtccg agacggccag ccgcgatccc gcgtttcgaa gtacgcgctt
     9421 gccactttga attcaatctg ttatatatgc ctcgttaaag gtgattgttt tgtcttccgg
     9481 cccggaggca gacgcacatt ccgaacccat tttccgtgaa gacccctggg cacggaggta
     9541 agctgatgaa aaaacaggcg cgtacagcac gggaacggaa agcgggccgc ccctcgtcag
     9601 cggatcgcaa gggcctctcc ccggctcgac gcccggaatc ggggaggcgc aagccgatcg
     9661 ctttgccgcc gaataacaag gcttcggcaa ccgcagccct ggtttctgcg cgggtcaggc
     9721 cgggcgggca gcaacgcgat tttacagcag aacagcaaca gaaagcgtat gacgaggcca
     9781 tgcgcctgtt ccaagcccat aaatttgagc gtgccgacga gttgttccga aaagcgacgg
     9841 aaggccccaa tcgcaccctg gcgcatcatg cccaaataca ctcccagatc tgtcaaaaga
     9901 ggcttgcgcc gcctgagccg aagctaaaaa ccgccgacga tcattacaac tatgccgtca
     9961 ccatgatgaa tgcccggcgg ctgaaggagg ctgccgagca tctcgagatg gcccttcata
    10021 tggcgccgca ggcagaccat ctttactatg ctttggcggc aaccgaggct ctccaaggaa
    10081 accctcaagg agcgtatgag cggctcaaga ccgcgattca tcttcagccg cgaaaccgca
    10141 tcctcgcccg cggcgatgcc gatttcgctg gaattttgga atacccgcct ctggcatcac
    10201 tcttgcagct tgatcggggc tggtcgccca aggcttctta gctgtcctct cgcggccggc
    10261 agttcgtagg gcactgctca tgaatcatga ttttgcgatc gtaatcctcg ccgccgggct
    10321 gggtaccaga atgaagtccc gccaagccaa agtactgcac cgcgcggggg gaaaaaccct
    10381 gattgagctc gtcacgtcca cggccgtacg cttggttccg cctgaacgcg tgtacgtggt
    10441 catcggacac cagggcggcc tggttcgcca ggttctcgac ggctccggcg tgcgcttcgt
    10501 ggaacaaacg gagcaactgg gaaccggcca cgctatcgcg agcggccgcg ggtgtctcga
    10561 cgcctccggg aagagactcc tggtgctgta tggagactgt ccccttgtca cgcccggaac
    10621 tctcgaagcg ttgatgcgcc ggcacgtgga atcgggtgct gcggccacag tcctgacaac
    10681 gacgctggag aatccttccg gctatggccg catcttgcgc ggcagttccg gagcagtcca
    10741 ggcgattgtt gaagaaaaag ccgccacccc ggagcaaaag tctatccgcg aaatcaattc
    10801 cgggatgtat tgctttgaga cggctccatt gtttgaagcg ctcgccgcgt tgcggcctga
    10861 caatccggcg aaagagtatt atttgacaga cgtcattgcg ggcatggtcg gacgcggtct
    10921 gaaagtggag gcctgccaca ccgcggagtc ctccgaagtt cttggcatca acacccgtgt
    10981 ggaattggct gccgtcgatg cggttctgcg ctcgcgggag gtccgcaggc tcatgctgga
    11041 gggcgtgacg atccagaagc cggaaactgt cattatcgac gctggcgtct cagtcggcga
    11101 ggataccgtc gtcgagccct tcgtgcaggt gctcgggcgt tgccggatcg cggcaaactg
    11161 ctggatccgc gcccactcca taatcagcga ttcagttctg ggcgagggcg tggtggttga
    11221 gccgttctct atcatccagg aatcggaggt tcagcgcggc gcccggatcg gacctttcgc
    11281 caggctccgt gcgggaaaca tagtaggagc agaggcccgg gtcggcaact tcgtggaact
    11341 caaaaagaca gcctttggcg cacgctcaaa agccatgcac cttgcctatc tcggagacgc
    11401 cgctgtgggc gcggacgtaa acgtcggggc aggcacgatt acctgcaact acgacggtac
    11461 agccaagcac ccgaccgtga tcgacgacgg g
//
LOCUS       DASREK010000297         8627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2123759, whole genome shotgun sequence.
ACCESSION   DASREK010000297 DASREK010000000
VERSION     DASREK010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123759"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..2255)
                     /locus_tag="VFA54_16040"
     CDS             complement(177..2255)
                     /locus_tag="VFA54_16040"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HZS52381.1"
                     /translation="MPYCIALFMEAAVFHRRVLFYRGFLFPWDFRGVHLPLAAFVADS
                     FRRGELPLWDPYTYCGYPIFANIQAALFYPPALAAALGSLLLGASSLPRLLAAAAALQ
                     ISFGGCCTFAILRRIGAGRPAAWIAGTTYELGCFFASQAQHMGAVHGASWLPLAWLCV
                     IELRASLRWGWLATLAGALAMSVFAGLPQVAVVVFGSALALGFLLAVLRLARPALPAY
                     VLLAWAWALLLAAVQIVPTLQLTLQSVAKYRYEWLRSGGGIKPGALWSLALPNYWNVF
                     DLSKFSGPSDPTFLYLYSGLLSLALAVAAACWKPDRWARALAWLAASAAVWMLGDSTP
                     IGRAIFLALPVNVRIGIHPEFTLCAFALALSLLAGCGANRFLGDTRVSVIAGVVIVCE
                     LILVSSGRPMNTASLAAEPGIMRDSADGSRELVERLRALTGRSMPPSRFDTTPSVSFL
                     WSTAAPMLGIPTANGCDPLAPERMIQVRLSFSPGARWGTCYQVVNASSPVVGLCNVRY
                     LLSRAPLPDRGLELAAEIAAYKIYENKEALPRFFLVNRVARVDRLSQAAALLHAPGFD
                     PAQTAIVEAPDRFEPLPAAPAHGEVEVISYRSTSAVLATHADSPSFLVAAEAYYPGWE
                     AAIDGERVPLYAADVAFRGIRVPAGDHRVEMRFVPRILYRSVVISMTAFTTALVVLLM
                     SARRRSKKSS"
     gene            2578..2823
                     /locus_tag="VFA54_16045"
     CDS             2578..2823
                     /locus_tag="VFA54_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52382.1"
                     /translation="MKWLLCAAAARLLQQFGLPNGAALTAGIDSPVTVAGARTSSFSR
                     VPVMLVSYTRALKIWLYRIRDGTTPRGMILWSRTAPV"
     gene            complement(3094..3429)
                     /gene="trxA"
                     /locus_tag="VFA54_16050"
     CDS             complement(3094..3429)
                     /gene="trxA"
                     /locus_tag="VFA54_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZS52383.1"
                     /translation="MAGANVLNFTDASFDSDVLKSDVPVLVDFWAEWCGPCRMMSPTI
                     DAIASEYAGKAKVGKLNVDDNPATAGRFQIRGIPTLLLFKGGAVAEQLVGAVGKDKVQ
                     SILNKHLGS"
     gene            complement(3496..4935)
                     /gene="glgA"
                     /locus_tag="VFA54_16055"
     CDS             complement(3496..4935)
                     /gene="glgA"
                     /locus_tag="VFA54_16055"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HZS52384.1"
                     /translation="MVASEAVPFSKTGGLADVVGALPQAVRSLGHEVAVVLPLYRMTR
                     LERPATLFPALTIPLGQNTCFPSVQTVAEGGVKFYFIAYPAFFDRAALYGTPQGDYPD
                     NAERFALLSRAAIEIAQLDFHPDVFHCHDWQAALVPVFLRSIYVKSPAVGQTPVLFTI
                     HNLGYQGLFRHEVLGQIGLPDSLFHIDGLEFYGKVNLLKGGLVFSDAINTVSRGYARE
                     IQTEEYGFGLDGLLRRRSRVLSGIINGVDYTQWDPATDKFLAANYSPQDLRGKRVCKA
                     DLLHEFGLPQENLDRPLIGIVSRLSAQKGADLIAEAAGELMSEDLAMVVLGSGDAVYE
                     DLFKGLASRYPAKLAVRIAYDNALAHKIEAGADIFLMPSRYEPCGLNQIYSLKYGAVP
                     VVRATGGLDDTVEPFDPATGRGTGFKFAEYSATAMLRALRAALEAYHNGPLWQKVMIN
                     GMSRDFSWGSSAAQYVKLYETLAATGRQT"
     gene            complement(5032..5442)
                     /locus_tag="VFA54_16060"
     CDS             complement(5032..5442)
                     /locus_tag="VFA54_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52385.1"
                     /translation="MDAFFKKDGVYRPAWKLVRVINTSLSTGVHLGSSGLRLTVAQPL
                     QGDWKLTGARLATFENAAVQDKLDLAQAPGGRAGEEWTFTSLTSAFTKALGGAKNTWL
                     SLDFISPAGNQMLRLMKPLPALDNRVAGIEHANE"
     gene            complement(5382..5786)
                     /locus_tag="VFA54_16065"
     CDS             complement(5382..5786)
                     /locus_tag="VFA54_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52386.1"
                     /translation="MPFWKTIQDHLMGSITAGGLVLVAAGAYWVDLHKEKILDWVAGV
                     QIEVSSTNRPSVSSFIAGEELRFSLKGVESDKVFWFLARMKKMWWRPEAQRSDIPFNL
                     ILRAAKMWNGTGAWMLFSKKTACIAPPGSWFE"
     gene            5905..7086
                     /gene="ribB"
                     /locus_tag="VFA54_16070"
     CDS             5905..7086
                     /gene="ribB"
                     /locus_tag="VFA54_16070"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZS52387.1"
                     /translation="MSFASVPEALEEMRAGRMIVVVDDEDRENEGDLTIAAEKVTPEV
                     INFMARYGRGLICLALTEERLDYLRLPLIAEQNTSLYGTAFCQSFDARQGVTTGISAA
                     DRARSIHVAIDPATTAADLARPGHIFPLRARKGGVLVRAGQTEASVDLARMAGLIPAG
                     VICEIMNDDGSMARVPQLMEFCARHGMKMLTVAELIRYRLQYERFLHRIAEGSVETPY
                     GEFHTIAYQSDLDTEVHVALVRGHVEDDESVLVRVHSHCLLGDVFLSAACGCHELIEQ
                     SLARIAAEGRGVLLYLHATGAGFNLETLPSGEKRLVSHSREYYHYLGEAGQRRQQYES
                     GVGAQILSDLKLRTIRLLTNHPRKIVALEGFGIRIVDQVPVTPPAEQEREMAARRPDH
                     S"
     gene            complement(7221..7559)
                     /locus_tag="VFA54_16075"
     CDS             complement(7221..7559)
                     /locus_tag="VFA54_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52388.1"
                     /translation="MDLPRVLRELYTERRCLETIIAALEIISRSPDERVGAALVAHLR
                     NSGWDGSMLRLGQSEKRELTRLARRVCRAGMAAGDEDGSTALPRVDHGAALAAAVIPC
                     IRSRSGIQAA"
     gene            8156..>8627
                     /locus_tag="VFA54_16080"
     CDS             8156..>8627
                     /locus_tag="VFA54_16080"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS52389.1"
                     /translation="MVQRTDMQTNRIKHILRAGGSILGTCVTDHTSPEIVTIFKAAGL
                     DFFFIDTEHSVPSYRDVQTLVRVGRAANVIPLARVTQSEYFLIARLLDVGAMGIIAPR
                     INSAAQGRAVVDAMKFTPEGSRGYGLRGIINDYQYAGAQAEMESANRETMVILQV"
BASE COUNT         1829 a         2635 c         2597 g         1566 t
ORIGIN      
        1 agttcctctg aacaccgcca aacagatcaa ctgagaaaac gagaaccgcg gatcgcctgg
       61 tttcctcatc ggctgggggc cgacaggtgt ttgccgcaga ttcttgcaaa ccgattcttt
      121 ccaaggctca gccacgtccc agcagtcagg aactgctgca ccggctgcgc ctgatttcac
      181 gaagattttt tcgagcggcg ccgggcggac atcaaaagca caaccagagc ggttgtgaaa
      241 gcagtcattg agatcaccac cgagcgatag agaattcgcg gaacgaagcg catctcgacc
      301 cggtggtccc ccgcgggaac ccggatgccg cggaatgcga catcggcggc atagagagga
      361 acgcgttcgc catcgatggc cgcttcccag cccggataat aggcctccgc ggccactaga
      421 aacgaagggg agtccgcatg ggtcgccagg acggcgctgg tggaacgata cgagatcacc
      481 tcgacctcgc catgggccgg cgcagcgggc agcggctcga atctgtccgg cgcctcgacg
      541 atcgccgtct gcgccggatc aaatccgggc gcatgaagca atgccgcagc ctgggaaagg
      601 cgatcgacgc gcgctacgcg gttcacgaga aaaaaacgcg gcagcgcctc tttgttctcg
      661 taaattttgt aggccgcgat ttccgccgcc agctccagcc cgcgatccgg caaaggcgcc
      721 cgagagagaa gataccggac attgcacagg ccgacgaccg gcgaggatgc attgacgacc
      781 tgatagcaag tgccccatct ggctccggga gaaaacgaaa ggcgaacctg gatcatccgc
      841 tctggcgcaa ggggatcgca accgttcgcg gtcggaattc ccagcatggg agcggctgtg
      901 gaccacagga atgacacgct gggggtcgtg tcgaatcgcg aaggcggcat cgagcggccc
      961 gtcaatgcgc gcagccgctc gaccagttcc cgactgccgt cggctgaatc gcgcatgatg
     1021 cccggctccg ctgccaggga cgccgtattc atcggacggc ctgaactgac gagtatcagc
     1081 tcgcagacaa tgaccacacc cgctatcacg gaaacccttg tgtctcccaa aaatcgattc
     1141 gcgccgcagc ccgccagtag agacagagcg agcgcgaagg cgcacagcgt gaactcggga
     1201 tgaattccaa tgcggacgtt caccggcagc gccagaaaga tcgcgcgccc gatcggagtg
     1261 gagtcgccga gcatccacac ggcggcgctg gccgccagcc acgcgagagc gcgggcccac
     1321 cggtccggct tccaacacgc agcagccacc gccagcgcca ggctcaacag gccggagtac
     1381 agatacaaaa atgtggggtc ggacgggccg ctaaatttcg atagatcgaa aacgttccag
     1441 taattgggga gcgcgagcga ccacagtgcg cccggcttga tgccgccgcc gctgcgcagc
     1501 cactcatacc ggtacttagc gacgctctgg agcgtgagtt ggagggtggg aacgatttgt
     1561 acggccgcca agagcagcgc ccacgcccac gcgagcagta cataagccgg gagggccggc
     1621 cgggccaggc gcaataccgc gagcaaaaac ccaagagcca acgcagaccc gaacacgacg
     1681 acggcaacct gcggcagtcc tgcgaagacg ctcatggcca gcgcgccagc gagcgttgcc
     1741 agccagcccc agcggagaga tgccctcaac tcaatcacgc acagccaggc tagcggcagc
     1801 cacgatgcgc catggacggc gcccatgtgc tgcgcctgcg aggcaaaaaa gcagcccagt
     1861 tcgtaagtcg tccccgcaat ccaggccgcc ggccgtccgg cgccgatccg ccgcagtatg
     1921 gcgaaggtgc agcaaccgcc gaaagaaatc tgaagcgctg cggctgccgc cagaagacgg
     1981 ggaagggacg atgcgccgag caataggctc cccagcgccg ccgcaagagc gggcggatag
     2041 aacaaggccg cctggatgtt ggcaaatatc gggtatccgc aatatgtata tggatcccaa
     2101 aggggcagct cgccgcgccg gaacgaatcc gcgacgaagg cagccagagg cagatgaaca
     2161 ccccggaagt cccacggaaa tagaaatccc cgatagaaca acacgcgacg gtgaaagacg
     2221 gccgcctcca tgaagagcgc gatgcaatag ggcacgctac ctgacaggca gcgcctgacg
     2281 gcggatcggg tgctggccat taggatgatt gggaatggaa ttcgccggtt gcaaacagac
     2341 gacgggcgaa tttttcgtct cggatggcgg cgccgccgtc ggccagcctt cgcgacaggc
     2401 cgaagagttt catgcaggcg aacggggtcg cgaaactccc aacgtacacg gtcatcgagc
     2461 gccgctgcca catgccttga ccaccgcagc cttgccggcg agctgcgggg gatccaaacc
     2521 caatataata ccccgcgggc aaaccgaaca ttcgttcgga gatgctgctc cggtggtgtg
     2581 aagtggctac tttgcgcggc ggctgcccgg ctgctccagc agtttggcct cccgaacgga
     2641 gcggcgctga cggcgggaat cgatagcccg gttaccgtgg cgggcgcgcg aacaagctct
     2701 tttagcaggg ttccggtgat gctcgtcagc tacacgaggg ccttgaagat ctggctctac
     2761 agaattcggg acgggacaac accgcgggga atgattcttt ggagccgcac ggcgccggtt
     2821 tgacgagtgc gccgccaggc tctgctaatc gccaggaggt tgtggccttc gtttccgatc
     2881 cgaagaaaag cctgtcttcg agattgttcg ggcaaaaagg ggcgcgagta tctcattggc
     2941 gcggaagcgc cgatcttcgc gcgggtcgca gatccacgga gcctcaaccc ttggcttgca
     3001 tgccggaaga tccactgccg ttccgagcca gacgtgcggc tcagcgccgg caattccttg
     3061 cagtttggct tcgaattggc ttgtttaaaa gaattagctg cccagatgct tgttcaggat
     3121 gctttggact ttgtctttgc cgaccgcacc gacaagctgc tccgcaacag cgccaccctt
     3181 gaacagcagc aaagtgggaa tgccgcggat ttggaagcgc ccagccgtgg caggattgtc
     3241 atcgacattc agcttgccca ccttggcctt gcctgcatat tcgctggcga tcgcgtcaat
     3301 agtaggggac atcatccggc aggggccgca ccactcagcc caaaaatcca ccaataccgg
     3361 cacgtcggat ttcaggacat ccgaatcaaa agaagcgtcg gtaaagttca gaacgtttgc
     3421 tccagccatt cgtcggttcc ctcacaggtc taaagtacga tcaccattat agatgaacag
     3481 atggggcgag aagactcagg tttggcggcc cgtggcggcc agggtttcgt aaagcttcac
     3541 gtactgggcg gccgaggagc cccaggaaaa gtcgcgcgac atgccattga tcatcacctt
     3601 ctgccaaagc ggaccattgt ggtaagcctc gagagcagcg cgcaaggccc gcaacatcgc
     3661 agtggccgag tattcggcga acttgaagcc tgtgcccctc cccgtagcgg gatcgaaggg
     3721 ctcaacagtg tcgtccagac ccccggtggc gcgcaccacg ggcacggcgc catacttcaa
     3781 gctatagatt tgatttagcc cgcaaggctc gtaccggctt ggcattaaga aaatatcagc
     3841 tccagcttcg atcttgtgcg ccagcgcgtt gtcataagca atgcggacag ccagcttcgc
     3901 cggataccgc gaggcaagtc ccttgaacag gtcttcgtag accgcatctc ccgagcctag
     3961 gacgaccata gccaagtctt ccgacattaa ctctccagcg gcctcggcga tcaggtctgc
     4021 ccccttttgc gcgctcagcc gggagacgat gccgatcaaa ggccgatcga ggttctcctg
     4081 tgggagaccg aattcgtgga gcaggtccgc cttgcacacc cgttttcccc tcaggtcctg
     4141 cgggctgtag ttggcggcca ggaatttatc ggtcgccggg tcccactggg tgtaatcgac
     4201 tccgttgatg atgccactca gtacgcggct gcggcggcgc agcagtccgt ccaggccgaa
     4261 gccatactct tcggtctgga tctcgcgggc ataaccccgg ctgacggtgt tgatggcgtc
     4321 ggaaaagacc aggccgccct tcagaaggtt tactttgccg tagaactcca ggccgtcgat
     4381 gtgaaacagc gagtcaggca atccaatctg gcccagcact tcgtgccgga acaagccctg
     4441 atacccgagg ttgtggatag tgaacagaac cggcgtttgc cccaccgccg gtgacttgac
     4501 gtagatcgag cggaggaaga cgggaaccag agcggcctgc cagtcatggc agtggaaaac
     4561 atcgggatga aaatcgagct gcgcgatctc aatggcggcc cggctcagca gcgcaaagcg
     4621 ctcggcatta tcggggtagt cgccttgggg cgtcccgtag agggccgcgc gatcaaaaaa
     4681 cgctgggtaa gcgatgaaat aaaatttcac cccgccctcg gcaacggtct gcaccgacgg
     4741 gaagcaggta ttctggccga gcggaatggt caaggccggg aacagggtcg ccgggcgctc
     4801 caggcgcgtc atacgataca gcggcagcac gacggccacc tcgtgtccga gactgcgcac
     4861 ggcctgcggc aacgcgccga ccacgtccgc aaggcctcct gttttggaga agggcaccgc
     4921 ttcggaagct accatgagga ttttcatgtg agttggatag gcgggcagaa ggcctaatct
     4981 tcaaactact gaattgcgct tagagaatgc aaggatcata attaccgtga gctattcgtt
     5041 ggcatgctcg atgccggcga cgcggttatc cagcgcgggc agcggcttca ttaaccggag
     5101 catctgattg cccgcaggcg aaataaaatc gaggctgagc cacgtatttt ttgcgccgcc
     5161 gagcgccttc gtgaaggcgg acgtcaagct ggtgaacgtc cattcctctc cagccctccc
     5221 gccgggagcc tgcgctaaat ccagcttgtc ctggacggcc gcgttttcga aggtcgccag
     5281 cctggcgccg gtgagtttcc aatctccctg caatggctgc gcaacagtca gcctcaagcc
     5341 ggacgacccg aggtgaacgc ccgtactgag agaggtattg atcactcgaa ccagcttcca
     5401 ggcggggcga tacacgccgt cttttttgaa aaaagcatcc acgcgccggt cccattccac
     5461 atttttgcgg cccttaggat caaattgaaa ggtatatcgg atctctgggc ctcgggtctc
     5521 caccacattt ttttcatcct cgccaaaaac caaaagacct tatccgattc gacgcctttc
     5581 aaggaaaacc ggagctcctc cccggcaatg aagctgctga cgctcggccg attagtggaa
     5641 gaaacttcga tttgcacgcc ggccacccag tcgagaattt tttccttgtg gaggtccacc
     5701 caatacgcgc ccgccgccac gagcaccagc ccgcctgcgg tgatcgaccc catgagatga
     5761 tcttgaatcg tcttccagaa cggcatccca acgcgctcct cgggaaatcg ggcgtattat
     5821 acaacggttt ccggatggcg ccaggaccgg cggagatttc ttgcttcccg ccgccccggc
     5881 ccgagcccgt gctagattgg aattatgtcc ttcgcaagcg tgccggaagc cctggaagaa
     5941 atgcgcgccg ggcgcatgat tgtagtcgtg gacgatgaag accgggaaaa cgaaggcgac
     6001 cttaccatag cagccgaaaa ggtcactccc gaggtcatca atttcatggc ccgatatggt
     6061 cggggcctga tctgcctcgc tctgaccgag gagcggctgg attatcttcg cctgccgctg
     6121 atcgcggagc aaaatacgtc tctttacggc acggcgtttt gccagtcatt cgacgcgcgg
     6181 caaggcgtta cgacgggcat ctcggccgcc gaccgcgcgc ggagcattca tgtggccatc
     6241 gatccggcca ctacggcggc cgacctcgcc cggcctgggc acattttccc gctccgcgcc
     6301 cggaagggcg gcgtactggt tcgcgcggga caaaccgagg cttccgtcga tctggcgcgc
     6361 atggcggggc tgatcccggc cggcgtgatc tgcgagatca tgaacgacga cggatccatg
     6421 gcccgcgtgc cgcaactaat ggaattttgc gcacggcatg gaatgaagat gctcaccgtg
     6481 gccgagctga tccggtaccg gctccagtat gaacgcttcc tgcaccgcat cgcggaaggc
     6541 tctgtcgaga ctccctatgg cgaatttcac acgattgctt atcagagcga tctcgataca
     6601 gaggtgcacg tggcgctggt acgcggtcat gtcgaggacg acgagtcggt gctggtgcgc
     6661 gtccattcgc attgcctgct gggagacgta ttcctttccg cggcctgcgg ctgccacgag
     6721 ctgatcgagc aatccctggc gcgcattgcg gcggaaggcc gcggagtgct gctttacctg
     6781 cacgccaccg gagcgggctt taacctggaa accctcccgt cgggcgagaa gcgcctcgtt
     6841 tcacacagca gggaatacta tcactactta ggagaagcgg ggcagcggcg gcagcagtac
     6901 gaatcggggg tgggagcgca gatcctttcc gatttgaagc tgcggacaat acgcctgctc
     6961 accaatcacc cgcgtaagat tgtcgctctg gagggcttcg gaatccgcat cgtcgatcag
     7021 gttccggtaa cacctcccgc cgagcaggag cgcgagatgg cagcccggcg accggaccac
     7081 tcctaaggag ccttatccac cgcgacgtgg cgaaaacggc gcgaggctcg gccggaaatt
     7141 gcgatggatt ttccggcggc ctcggcgggt ttcccttggc ggaaccatgc gccaatagac
     7201 tgaggtcggg cgccgaaacc ttaagcagcc tggataccgc tccgggagcg gatgcagggg
     7261 ataacagccg ccgccagggc cgcgccatga tccacgcgcg gcagcgcagt gctgccatcc
     7321 tcgtccccgg cggccatgcc ggctcggcag acccggcggg ccaagcgggt cagttctcgc
     7381 ttttcgctct gtccgagacg cagcatgctg ccgtcccagc cgctgttgcg caggtgtgct
     7441 acgagcgcag cccccacccg ctcatccggc gaccgggaaa tgatctccag agctgcgatg
     7501 atcgtctcca ggcagcgcct ctcggtgtac aattctcgca gcaccctcgg taggtccata
     7561 ccaagccccc cattctctgc ttctgacaac ctggctggga agatccaggc gccggttctc
     7621 tccagcgccc tccgtccctg gattcccgcg gcctgatgcg cgcccggggc ccaggctgct
     7681 attgcaactg cactgttttg tgtccgcccg gtgattcgga cgaagccatc cttttgttac
     7741 gtttcccagg ttcgacagag catttatata ccctgtccca ttgggagtca atataaatgt
     7801 gaggaaaaaa tgtgaataac gtagaactcc cggctaacta ccgttgcaga ttttcgtttg
     7861 tcagagtgaa tagattagga ataaaagata ttttcctgaa ttttacccct aattccggca
     7921 cagtgggtgg gtacgccacc gcggcccatc cgtttaggta tctggagagc ggcaaacggt
     7981 ctttccgcgc ggttcactgc gatcccagga cgccccccaa aaggtcgaat ccaaacggac
     8041 gcgcgagggc aaccaacggg ctgggccgaa gttgaggcgg tcttcgccgc atgggccatt
     8101 gccgtcgctc tctagtccgg gacgcccgat tcgcacttca tgccccggca tcgttatggt
     8161 acaacgtacg gatatgcaaa ccaaccgcat aaagcacatc ttgcgcgccg gcggctcaat
     8221 tttgggcacc tgcgtcaccg atcacacttc gcctgaaatc gttacgattt ttaaagcagc
     8281 gggcctggac tttttcttca ttgataccga gcattccgtt ccgagttatc gggatgtaca
     8341 gaccttggtc cgggtcggcc gggccgccaa tgtcatcccc ttggcacgag tcacccagag
     8401 cgaatatttt ttgattgccc ggctgctcga tgtaggcgcc atgggcatca tcgctccgcg
     8461 gatcaactcc gcggcccagg gccgcgctgt tgtggacgcc atgaagttta cgccggaagg
     8521 cagccggggc tacgggctgc gcggaattat caatgattat cagtatgcgg gcgcccaggc
     8581 cgaaatggaa tcggccaacc gggagaccat ggtgattttg caggtag
//
LOCUS       DASREK010000298        26045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_636177, whole genome shotgun sequence.
ACCESSION   DASREK010000298 DASREK010000000
VERSION     DASREK010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 26045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26045
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636177"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..478
                     /locus_tag="VFA54_16085"
     CDS             122..478
                     /locus_tag="VFA54_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HZS52390.1"
                     /translation="MSSSTCTSVILWRWEHSFLTLEDLARAADLHPTLVESFVSYGLL
                     EPAAESGGAPLFAPAAVGRLRRIVRLRRDLGVNLAGIAVILEMRDRLERLQKEVAMLR
                     ERTGRRERRTTARRTP"
     gene            481..1314
                     /locus_tag="VFA54_16090"
     CDS             481..1314
                     /locus_tag="VFA54_16090"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZS52391.1"
                     /translation="MVPSDLLWLFFVLSALQPVLKQRLLEASRQRLIAKIERQRNSRV
                     ILLVHRQETMSILGFPVFRYIDVNDSEEVLRAIHLTDPEVPLDIVLHTPGGLVLAALQ
                     IARAIYKHPGKVTAFVPHYAMSGGTLIALAADEIVMSEYAVLGPVDPQLGQYPAASIL
                     KAVARKPVAEVKDETLILADQAEKAMAQVRESVKELLAKKCPEEKAEELARLLSEGTW
                     THDHPITYERAKSFGLPVRCDMPAEFLDLMNLYPQPVRRQPTVEYLPVPRRYEGFRNV
                     E"
     gene            1330..1698
                     /locus_tag="VFA54_16095"
     CDS             1330..1698
                     /locus_tag="VFA54_16095"
                     /inference="COORDINATES: protein motif:HMM:NF041086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAUR_1299 family protein"
                     /protein_id="HZS52392.1"
                     /translation="MADYLPLLFHKAFRVIPGADYNRTLEELTSGAKSIERAPFYEVA
                     LDEGKPWEYLRDRVYPSFVRYLKNKGIDPENPEQVTVAVFYRDQCYLVGGPQFIEMFQ
                     EMEGINPAAFHFRVLQWLAG"
     gene            1873..2340
                     /locus_tag="VFA54_16100"
     CDS             1873..2340
                     /locus_tag="VFA54_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HZS52393.1"
                     /translation="MLNTLLMDDVRRTFDVLRRSMDEFFGSVWTASTFDGSESTFAPN
                     FEAGWTGDRLNLRVVLPGVSEKDVKVTVQGNQLLVEGERKAPENFSHDGFSSVLPYGR
                     FYRAISLPAGLDLDKVSGRLHDGVLDIEIPVKEEMKPRVIAIEGAESRKALPV"
     gene            complement(2544..2885)
                     /locus_tag="VFA54_16105"
     CDS             complement(2544..2885)
                     /locus_tag="VFA54_16105"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZS52394.1"
                     /translation="MPFHNSRHDSGAQVIRFEGRITFGGETDACRQFLKHLLADGERE
                     FIFDLTRVEYIDSAGIGFLVSCLTTLAHDGASLRLASPTARVQYVLGITKLYTVFQIF
                     PTLDQALRNRP"
     gene            3037..3441
                     /locus_tag="VFA54_16110"
     CDS             3037..3441
                     /locus_tag="VFA54_16110"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HZS52395.1"
                     /translation="MIEFGMAFPLLFAFLIGMFQLGYGCFLYNQLQMVVRTGARYASI
                     ADFDSASSGASFRSNVANMVVYGNPSGGTRPLVTGLRTSLVSVTWQADGAGIPQTVTV
                     KISSYSFSVLGKAYQLTNKPQATFIYLGQFLS"
     gene            3508..3894
                     /locus_tag="VFA54_16115"
     CDS             3508..3894
                     /locus_tag="VFA54_16115"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HZS52396.1"
                     /translation="MVETALILAAALITFIGIIDVGSVLFRLQGLVERARAGARYAVV
                     NSFDATKIQNVVVFGNSAGNGNPLLGLQPGFVTANLIDLGDGNEKVQVIISGYTFNFF
                     TPYIAGSKTFPTVEADLTTESLGAAN"
     gene            complement(3904..5124)
                     /locus_tag="VFA54_16120"
     CDS             complement(3904..5124)
                     /locus_tag="VFA54_16120"
                     /inference="COORDINATES: protein motif:HMM:NF021493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZS52397.1"
                     /translation="MTSAFKNRYGAASRGFVLVTAAVSVVAILGMLGLCLDLARLYIA
                     KNELQNYADAAAIAAANRLDGTSAGIANAISEAQTNVNKWYFDSSSVSSVNVDFATSP
                     GGAFLSNPSPATGYAFTRVQVQGNVPVYFLPIFAGIGASSTVKATAVAGQTLLSGLGD
                     GAFPYSPDAHVPNPLPADPTGNFGFIKGQLYTLRWDPVGKGSKVGITDKSGNKLVGCA
                     GDMNTPGFIPGSDNNGQRGYIDLLNGGGGGGANFIRNAILGNVEIPNPIQVGQTIDNA
                     SGNKQTEVTSLLTRVAQDTNPTTPTYYTAPASAVGEDPPGRTYYSVDPPGQPPANPPH
                     GNGRRIVTVPVNNPTNDVVMGFAQFFLPPVPCPGSNPQPCCAEYIGPASALPAGGGAN
                     GGGSLGLYRIRLFQ"
     gene            5369..7261
                     /locus_tag="VFA54_16125"
     CDS             5369..7261
                     /locus_tag="VFA54_16125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52398.1"
                     /translation="MTAATIVSSQGLQIGRGVITTAVGTGDKDPDGNGFYNGDGKPGP
                     QTQLNFYIGADDLEDFAHLAFDSAGNLYFPDKGNQLIRKLDKNGIVTTIAGSPGVDGF
                     NGDQADAKKATLSFPTGIFVDKAGNIFFTDEDAGVQQGGLVRKIDTTGALTTVAGSGK
                     FGYDGDTKPATQAALNGPTAVAVDTDGTIYFTDQFNDLVRKVDPKSGMITTVAGNGGH
                     GNSQAGADGVPGTSVTLGWPSGLALDGKGDLFIADQTNNVIRVLNLQTGLIRRIAGTG
                     QHSDDNPGGNGGPATQAALGYPIGLVLDPATGDLYFADMHNNAIRKITAPLTANAIIT
                     TIAGTGFFGFAGDGGPASAAQLARPAGLTLDANGNLYFMDWYNQRIRKVTPGSGIPQP
                     VVFSGGVVSAGGFAPAPAPVAPGSIVAIFGRNLASGIGFAAATPLPTTLLDSQPVSVS
                     VTAGSGMVSMPLFFVSPDQINAQLPFEVAPGQATLTVNVGGVQSAPVTFQVSSTATGI
                     FTYPGTNRAVAVNQDGSLNSFSQPAARGSIITVYLTGPGDLNPPFATGQGAPSNPLSQ
                     TKSSHSAIIGGKLSQINYLGATPGYVGLSQANIVVPDNAPVGDQQVVLHVGDQDSGNR
                     PVVTVK"
     gene            complement(7289..7879)
                     /locus_tag="VFA54_16130"
     CDS             complement(7289..7879)
                     /locus_tag="VFA54_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52399.1"
                     /translation="MKSRWRAAALILMMLALIVAFRVFGPRDVPEGQPALVSITDQTF
                     SDFQRVFNDSAETTRILVLFSPSEKRSLQGASALQATLEDFPKARLRVLVVWEPVERT
                     DFFAPTSDVLARISDRRAVQFWDRNRLVSQRIKAAGIPSVLRGNTIWDFLALFPPGAR
                     WDSAMPTPVLRGAPVVNVMEGLSRYLYSNSDPAASP"
     gene            complement(7904..8704)
                     /locus_tag="VFA54_16135"
     CDS             complement(7904..8704)
                     /locus_tag="VFA54_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZS52400.1"
                     /translation="MDLQVQLRRLQQIGLNAYESRAYLTLIGHPRFKALELATRAHVP
                     RQKIYEVLGSLVEKGFAQVVHGKAKLFSAVEPKLALEGYLARRREVFERELDDRRRLA
                     EMLHQDLHSVFIDGHQDRGPLDYLRIVNESGQISGEYRRLLARSTREYLEFSRRPYAV
                     DPVSEPRVREALERNVECRLIFESRDMNPVHRKNLRALVKYGAQIRVDSELPLKLALF
                     DGRFGMIALVDPVLTRPQITALVFEHDALAAALRRLFEDHWQRAQPLL"
     gene            8787..9578
                     /locus_tag="VFA54_16140"
     CDS             8787..9578
                     /locus_tag="VFA54_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS52401.1"
                     /translation="MFKEPIFRDRVAVITGGGTGLGRAFALRFSELGARVTLASRDSS
                     HLEPARAEIRRRGGEALAVPTDVRIPEQVENMVRETLRCFGRLDILINNAAGNFLCKA
                     EKLSYNGWRSAVDIVLNGSFYCSRAALEPMKIQRYGRILNILATYATGASPGTVHSCA
                     AKAGVLAMTRTLAVEWARYNVHVNAVAPGMFPTEGASSRLMPAPGAGERALQRIPAGR
                     AGRHEELANLAAYLCSDYADYINGESVAIDGGASWNHPSFEWENL"
     gene            9575..11071
                     /locus_tag="VFA54_16145"
     CDS             9575..11071
                     /locus_tag="VFA54_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HZS52402.1"
                     /translation="MKPSSYHLPATLGELRQSEFSQERIQNRTVKDEMRANLICRMER
                     GEPLFPGIIGYDDTVIPQVVNAILSRHHFILLGLRGQAKTRLLRALTELLDPVLPYLA
                     GCEIRDNPYAPICQHCRLLVAEKGDAAPIAYLPPELRYVEKLATPDVTIADMIGDIDP
                     IKAARGGLDLSSELTIHYGLLPRAHRGIFAINELPDLAGKIQVGLFNIMQEGDIQIKG
                     YPVRLPLDVLMVFTANPEDYTARGKIITPLKDRIGSEIRTHYPVTLDHGISITEQEAW
                     TDRRPRGGICIPQYIKEIVESIAFLAREEKKIDKRSGVSQRLSITCLENVVSNAERRA
                     LRNKERLVVPRVSDIYAALPSLTGKFELEYEGELKGADAVARELIKAAVGRIYDRYFD
                     GVNVSQIVQWFELGGTLKIRDAQPAAELLKQLEGIQGLMEAVKTLETGLPKDPAVKAS
                     AAEFVLDGLYAHRRISRSEELGYMAEEKKREPRDHDEFPPRGFRKHYN"
     gene            11089..12345
                     /locus_tag="VFA54_16150"
     CDS             11089..12345
                     /locus_tag="VFA54_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS52403.1"
                     /translation="MKFTTYSKYTGDDLGLSAEDLLQALADFLLRSGFDQQYMHFHEM
                     GEQTLEDLKQAIQEALENGSLFNNENMRELMEQLQQMSAEQMEKLLDRLIEKMAEEGY
                     ISIGEPNPQGERNVKFNPEGGVGENREGRVRFELTDKALDFLGFKTLKDLLASLGKAS
                     FGRHDTRDLATGIETTGASKPYEFGDTLNLDISNTLFNAIKREGLRVPLPLDYPDLMV
                     HQSEYQSSCATVLMLDCSHSMILYGEDRFTPAKKVALALSHLIRVQYPGDSLHLVLFH
                     DSAEEVPLSQLARVQVGPFYTNTREGLIVAQRILARQHKDMRQIIMITDGKPSALTLD
                     DGRIYKNAFGLDPLVVGRTLEEVHKCKRQGILINTFMLASDYGLVQFVQKVTEICRGK
                     AYFTTPYTLGQYLLMDYMNRKTRTIH"
     gene            12566..13048
                     /locus_tag="VFA54_16155"
     CDS             12566..13048
                     /locus_tag="VFA54_16155"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS52404.1"
                     /translation="MPTVKAGVQAPDFTLPDLTGRPQALSALLASGDVLIAFFKVSCP
                     TCQYTFPFLERLHKQSEARRLRVYGISQDDRQKSAKFAQQFGVSFPILLDGEEEDYPV
                     SNAYGITHVPSLFLIDPGGRIVLTSVGFCKADLEEMAQRGGTLPLFHRDEKIEAFRAG
                     "
     gene            13087..13260
                     /locus_tag="VFA54_16160"
     CDS             13087..13260
                     /locus_tag="VFA54_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52405.1"
                     /translation="MKKQPARQRNRFRAAKEVRKLARERIGAVPPGRVIEPKPRRKAA
                     KHPLKELERELEA"
     gene            complement(13285..13830)
                     /locus_tag="VFA54_16165"
     CDS             complement(13285..13830)
                     /locus_tag="VFA54_16165"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZS52406.1"
                     /translation="MAVREILQLGNPALRERSRPVVNFQAALAALQDLRDTLHDFQQR
                     YGFGRGISAVQIGMPERLIYLEIAGSSYSLINPVLRRAGESKILVWDDCFSFPQIMVR
                     LVRAERVQIDYADHRGVACSLAAEWDFAELLQHELDHLDGILAIDRAIDQNSFATRAE
                     FERRYCQERIVRLREGASSGA"
     gene            13968..14576
                     /locus_tag="VFA54_16170"
     CDS             13968..14576
                     /locus_tag="VFA54_16170"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZS52407.1"
                     /translation="MNVFQGHTGWLEVICGPMFSGKSEELIRRMRRGMIARKRVQVFK
                     PAIDNRYSSDEIVSHSDLRIPSQTVNSAREILELLEIRSELIGIDEANFLGPELPEVA
                     SRLADMGKQVIVAGLDTDYLGRPFEPIPSLLALAEEITKTLAICMQCGGPAKFTQRLV
                     ASDDLIVVGAAGLYEARCRRCFEAGSPRQHALDFVKPGSGKV"
     gene            14593..15492
                     /locus_tag="VFA54_16175"
     CDS             14593..15492
                     /locus_tag="VFA54_16175"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HZS52408.1"
                     /translation="MPSMFPVTPEPEQSPPAKTPFLEGRIGRSLIRLAIFLVFTFALW
                     WLVAGLMAPFAGPLVTSAISLGATALVTTAVMMRIYEMQPLSAVGLFWNRLGGIHLAL
                     GLGLGSGMSLLITAIQWSCGWARFQREPLPPDAGLTIPFWIFVLAIGAAGEELLFRGY
                     AFQNLIRAFGPWFSVISTSVLFGLGHSANPAFSRVSLLNTALFGLLFGYAYWRTRDLW
                     LPLGMHFAWNFTLAAIGANISGLKIRLMGLSVSSAGSCLWSGGDYGPEASLITTLVLT
                     AVVIFLWKAPTARQEDGILAGLP"
     gene            15501..16259
                     /locus_tag="VFA54_16180"
     CDS             15501..16259
                     /locus_tag="VFA54_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52409.1"
                     /translation="MKAFWAGLLILGLSAATGEAKLTFEERTELVRGLMAEFATAKVV
                     LPRSKKPLEVTPAGKYDKNVWAEAMQENGPAARVGDMVQITKVTIENDRIVLEINNGI
                     KGKHRWWHNVQITGATGNTSPIVADQNTNAPSGTTVALVFGDLSKPPKAADIKKMLQP
                     VMDFDKHTATELYTQTLPKEIQDAIKAKKVLVGMDREMVLLARGHPDRKLRENKDGVE
                     TEDWIYGKAPGTITFVTFQGNNVIRVKDDYAAPE"
     gene            16302..17312
                     /gene="gap"
                     /locus_tag="VFA54_16185"
     CDS             16302..17312
                     /gene="gap"
                     /locus_tag="VFA54_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZS52410.1"
                     /translation="MAAVKVGINGFGRIGRNIYRAAMHREGIDIVAVNDITDTKTLAY
                     LLKYDSVLGNLEAEVKAAGECIQVDGRQLRVMAQRDPAQIDWPSTGAQIVVESTGLFT
                     NAADARKHLRGTVKKVIISAPAKGEDLTIVLGVNDNKYDPARHSILSNASCTTNCLAP
                     VAKVLHDSFGVAKGTMTTIHSYTNDQQILDYPHKDLRRARAAALNMIPTKTGAASAIG
                     LVIPELNGKLDGFAMRVPTPDVSVVDLVVVTERPVTEKEVNQRLQEAAEGSMRGILGY
                     TEDPVVSTDFLHDPRSSIVDGAMTKVLDGNLVKVLAWYDNEWGFSCRMCDLIEMLANR
                     GL"
     gene            17313..18494
                     /locus_tag="VFA54_16190"
     CDS             17313..18494
                     /locus_tag="VFA54_16190"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZS52411.1"
                     /translation="MALSLRDLEPLLPGKRVFLRVDFNVPLDESGTRISSDKRIRASL
                     PSIRYVLEHKAALFLASHLGRPKGKPNPRMSLKPVAQRLSELLGQPVTLAPDCIGPAV
                     PAPAPGQVVLLENLRFHPEEESNDPEFSRQLASLCDVYVNDAFGSAHRAHASTAGMVA
                     FVRHAAAGFLMEKELKYLSMAVSNPSRPYVAILGGAKVSDKIEVIQNLLKLVDRLLIG
                     GGMAYTFLKSQGLAVGRSLVEEDKLDLAHELLRSAGDKLRLPSDHVVAAELKAEALSK
                     VVDGARAPIPDSWMAVDIGPETITAYSREAARASLIVWNGPMGVFEIAPFAKGTVELA
                     KAIATSKATSIVGGGDSEKAIQAAGVADKITHISTGGGATLEFLAGETLPGVAALEKK
                     A"
     gene            complement(18498..19220)
                     /locus_tag="VFA54_16195"
     CDS             complement(18498..19220)
                     /locus_tag="VFA54_16195"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS52412.1"
                     /translation="MNPVKLSVVIPVYNEKDTIGEIVSRVQRVNISKEIIIVDDGSTD
                     GTRERLQQIQSEQGNVHVIFQPRNMGKGKALRAGFAAAAGEYVIVQDADLEYDPEDYH
                     KLLGPLERQQADAVYGSRFITTEEHRVLYFWHSMGNKLLTLLSNMATDLNLSDMETCY
                     KVFRRELIQSIELEEDRFGFEPEITCKLARARARIYEVGIAYHGRTYEEGKKIGFKDG
                     LRALWCILKYRFKPLRRKKPAL"
     gene            complement(19318..19914)
                     /locus_tag="VFA54_16200"
     CDS             complement(19318..19914)
                     /locus_tag="VFA54_16200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HZS52413.1"
                     /translation="MVIRCATSNAHKLAEYELAAPPEWRIVPAGDCDCPETGSSFEEN
                     AIQKALCYAGHIGAPVFSDDSGLEVDQLGGEPGVNSARYAGSGASDEENRRRLLERLH
                     LSPGCGAPRPAARFVCVIALALPGRLLATFTGTAEGEIVDTPRGSGGFGYDPLFYFPP
                     LGRTFAELSPREKLAHSHRGKAFRQMLGWLKLHRAELR"
     gene            complement(19908..20624)
                     /gene="rph"
                     /locus_tag="VFA54_16205"
     CDS             complement(19908..20624)
                     /gene="rph"
                     /locus_tag="VFA54_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZS52414.1"
                     /translation="MRSDKRLPDQARPVRITPHYLLTAEGSALIEVGNTRVLCTASLE
                     DMVPNFLRGSGTGWITAEYSMLPRATVTRTPRESVKGRLSGRTQEIQRLIGRSLRAVA
                     DLALLGERTVIVDCDVLQADGGTRTASITGAFVALALALKQLVDSKILSRIPLRDYVA
                     ATSVGLLDGVPTLDLCYEEDSKAGVDMNVVMTGGGRFVEVQATGEHALFDERQLQVML
                     ALAAKGIQDLIGAERQVVSW"
     gene            complement(20706..20843)
                     /locus_tag="VFA54_16210"
     CDS             complement(20706..20843)
                     /locus_tag="VFA54_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52415.1"
                     /translation="MAKWRPAGTRKKPESNRARQIGCIFWLVVAMAFVMWLFYAMIKS
                     S"
     gene            complement(20941..22065)
                     /locus_tag="VFA54_16215"
     CDS             complement(20941..22065)
                     /locus_tag="VFA54_16215"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS52416.1"
                     /translation="MINSASPTLLLTGATGLVGGELLERLLAADPQRRIIALTRRPAG
                     AERLNQNPNVTVVAADLAQPSLGLDSALLADLERSLTEMIHTAADIRFGLPVEQARAA
                     NVTPTLHLLELARRCRRLQKFAHISTVYVAGRRSGVIAEERLSAHNGFLNTYQQSKYE
                     AEELVFRFMGEVPAVIFRLSSIIGDSTTGRVRQFNHVHQLLRLLPRNVLPLAPGDPQA
                     PVDLISSDWAVAALAYLFENRFVPGRVYQLCAGPDASLTVSQMIDLTVDLMESHPACR
                     RNGRIRLPKLVTLAEYQRFVEQTRRSGDVLLNELLRVLGYFLPHLGLFQAFENRNTAA
                     ALQGSGLALSPIRSYYDKVVRYCLDTDWGRRPERLAGNAV"
     gene            complement(22187..22939)
                     /locus_tag="VFA54_16220"
     CDS             complement(22187..22939)
                     /locus_tag="VFA54_16220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZS52417.1"
                     /translation="MVLELGIGAPLTAALAAGYGVRGRSSPLFGPNLWRGPREKRQIA
                     LTFDDGPSEATLPILELLELYSARATFFQCGKNVERLPEVSRRVQDGGHEIGNHTYSH
                     PRLLGCTPSRIREEIGRTQQAISRAAGTAPRLFRAPYGIRWFGLRPVLRQFGLTGVMW
                     TVIAYDWEWEADEIAWHVISHASNGAIFCMHDGDRVSERVDRRNTIRALRRILPELAG
                     LGYCFVTAREMMDPRAPENSSAQNPGKASSPA"
     gene            22962..24704
                     /gene="polX"
                     /locus_tag="VFA54_16225"
     CDS             22962..24704
                     /gene="polX"
                     /locus_tag="VFA54_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HZS52418.1"
                     /translation="MPISLENSEIAALLYETADLMEIAGEDPFRIRSYRNGASAIETC
                     PERIADIVTNPERDVTEIRGIGKGLASVLKEIVEKGSFERRDQLLQKYPAGALELLKI
                     QGLGPKGIALLYSHYQVSNIDDLERVCREGKLRELPRMGEKLEQKILRSIEQYRRSAS
                     RFLIDHADRVAGEIAAYLAETPGVEKVTPAGSLRRRKETIGDIDLLVTGPDPAPALEK
                     FIKFPKIREVLAKGENKTSAKIGHEGIQVDVRVLDRSSYGSALQYFTGSKDHNVAIRT
                     RAIKMGFKLSEYGLFRATDEVKVAGSTEEEVYCTLGLAWIPPELRENTGEIEAAAEGR
                     LPALVELPQIRGDVHAHTTATDGRNSIAEMAAAAQAAGYQYLAITDHSKALAMANGLD
                     EKRLVEQIAEIRRVALDFPGLTLLAGCECDIRQDGAMDLDDEALAQLDIVIASVHSHM
                     NLDAGAMTERLLRAMENPHVNIIGHPTGRLLLRREPFPFDFERVAAEAARRNVHFEIN
                     ASPERLDLSANLLRIAKGKGVKFVISTDAHHTRHLANMKYGVWMARRGWLGPEDVLNT
                     LDARQFRAAMVKRR"
     gene            24797..25324
                     /locus_tag="VFA54_16230"
     CDS             24797..25324
                     /locus_tag="VFA54_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HZS52419.1"
                     /translation="MVELQYPIGRFQFEGDVTAAQRRDWIAQIAAAPGQLREAVRGLS
                     AQQLDTPYRPGGWTLRQVVHHLPDSHLNSYSRFRLALTEDNPTIKPYDQSRWAALEDA
                     RSAPVEASLRLFQGLHERWVLLLQSMSEADFARTYVHPERGVVHLGREVGLYAWHGRH
                     HVAHITSLRERLGWR"
     gene            25315..25860
                     /locus_tag="VFA54_16235"
     CDS             25315..25860
                     /locus_tag="VFA54_16235"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZS52420.1"
                     /translation="MAVKRAGLFAIRSSREILRARVFTVRRERAVEPGGIAVEREIVH
                     HSGSAVMLARDEQGRILLVRQFRLPARRKLWELPAGRLNDGETPLRAAQRELEEETGY
                     HAQSWKRLVAFYPSPGYCTEYMTVYLAEGLTPGATHPDEDESIETRWFTPEQIRQMIA
                     RGRIEDGKTLAGVLFLFAFQK"
BASE COUNT         5341 a         7749 c         8038 g         4917 t
ORIGIN      
        1 ccaatatgtg cggctggaga tagtgacgcc gcccaaccct tccgagccgg aaagggagtt
       61 attccgcaag ctggcggagg tctcccgctt cgatccccgg agcctgaaga ggtgaacagg
      121 catgagctcc tccacctgca cttcggtgat cctgtggcgc tgggagcatt cattcctgac
      181 tttagaagat ttggccagag ccgccgatct ccacccgacg ctggttgaga gcttcgtttc
      241 gtatgggctc ctggagccag ccgccgaatc gggaggggcg ccgttgttcg ctcccgcggc
      301 agtgggacgt ctgaggcgca tcgtcaggct ccggcgcgat ttgggcgtca atctggcggg
      361 catcgcggtg attctcgaaa tgagggaccg cctggaaagg ctccagaaag aagtggcgat
      421 gctccgtgag cgcacagggc ggagagaacg tagaacaact gcgaggagga caccgtaaaa
      481 atggtaccca gcgatttgct ctggctgttt tttgtacttt cggcgctaca gcctgtcctc
      541 aagcaacggc tgctggaggc atcgcggcag cggctaatcg cgaaaatcga gcgccagcgg
      601 aattcgcgcg tcattttgct ggtccaccgg caggaaacaa tgagcattct gggcttccct
      661 gtgtttcgct acatcgacgt caacgactcc gaggaagtgc tacgcgccat acatctgacc
      721 gatcccgagg tgccgctgga cattgttctc cacacgccgg ggggactggt gcttgcggcc
      781 ttgcagatcg cacgggccat ctacaagcat ccgggaaaag taacagcgtt cgtgccccat
      841 tacgcgatgt cgggcggaac tttgatcgct ctggcagccg acgagatcgt gatgagtgag
      901 tatgcggtgc tcggacccgt ggacccccaa ttggggcagt atcccgcggc gtctatttta
      961 aaggcagtgg cccgcaagcc cgtcgccgag gtcaaggacg agacgctgat cctggcagac
     1021 caagccgaaa aagccatggc gcaagttcgg gaaagcgtta aggaactgct ggcgaagaaa
     1081 tgccctgagg aaaaagcgga agaactggcg cgcctgcttt cggagggcac atggactcac
     1141 gatcatccaa tcacatacga gagagcgaaa agtttcggct tgccggtccg ctgcgacatg
     1201 ccggctgaat tcttggattt gatgaatctt tacccccagc cagtgcgccg gcagcccact
     1261 gtcgaatacc tgcctgtgcc acggcgctac gagggctttc ggaacgttga atgaagcggg
     1321 cgcgcaagta tggctgacta tctccctctg cttttccata aggcattccg ggtgatcccc
     1381 ggagccgatt acaaccggac cctggaggaa ctgacctcgg gagcaaaatc catagaaaga
     1441 gcgccgtttt acgaagtcgc tttagacgag ggaaagccct gggagtatct gcgggaccgg
     1501 gtgtacccct cgtttgttcg ttatctgaag aacaaaggca ttgacccgga gaatccggag
     1561 caagttaccg tggcggtctt ttatcgcgac caatgttatt tggtcggagg gccacagttt
     1621 atcgaaatgt tccaagagat ggagggaatc aacccggccg ctttccactt tcgcgtgttg
     1681 cagtggctgg ccggctaacc gccgcctccg ccctggtgat ttttcctccc gaagatctca
     1741 gcaaggcact ctcatattcg ctgcaaacag gacctgcaac tttttacaac ttgcagcgtc
     1801 taaggcaagt gcagggggtc ggcgagatac cctgtaccga atgaagtcca ttgaaagaag
     1861 gaggtgctgt ctatgctcaa cactcttttg atggatgacg ttcgcaggac gtttgacgtc
     1921 ttacgccgat cgatggatga gttctttggc agcgtatgga ccgcttcaac cttcgacggc
     1981 agtgagtcta ccttcgcgcc aaacttcgag gcgggatgga caggggatag gctgaatctc
     2041 cgcgtcgtcc tcccgggcgt ctccgagaag gacgtgaagg tcacggttca gggcaaccag
     2101 cttctcgtcg aaggcgagcg caaggctccg gagaacttct cccacgacgg cttctcttca
     2161 gtccttccct acggcaggtt ctatcgcgcc atcagtttgc ctgccggcct ggatctcgac
     2221 aaagtgagcg gccggctgca tgacggcgtg cttgacatcg agattccggt gaaggaagag
     2281 atgaagccgc gggtcatcgc catcgagggt gcggaaagcc gcaaggcgtt gcccgtgtga
     2341 acgtcgcgtc ctgaggcggg gcgaacgcga aagccggctt gccccgcctg caaaactcta
     2401 cattccgcgg cgacgaagaa cgccgagacc tgcaaagcaa cggcttcaaa tccggtcgaa
     2461 aaaaggccgc tgcgcaaacc cattccaact catcaccaga gagccggccg cacggaattc
     2521 gggccaagtg ccgaatggct tgcctaaggc cggttccgga gggcctgatc gagcgttggg
     2581 aaaatttgga aaacggtgta cagcttcgta atccctaaga cgtactgaac gcgcgcggtc
     2641 gggcttgcga ggcgcagcga agctccgtca tgagcgagcg tggtcagaca gctgaccagg
     2701 aagccgatcc ccgcgctgtc gatatactcc actcgggtca ggtcgaatat gaattcgcgc
     2761 tcgccgtccg cgagaaggtg tttcaaaaac tggcggcacg catccgtctc cccgccgaaa
     2821 gtgatcctgc cttcaaatcg aatgacttga gcgccggaat cgtgcctgga attgtggaag
     2881 ggcatcgatg taaggtgttc tgcctactct aagacaattc cggcggcggt tcaaccgatc
     2941 cggcccaaac tttatgctga tttcggccga taacatccag gaagggcctt ttcgatgtac
     3001 accgccagac gcggaagacg tgagcgcggg aacacgatga tcgaatttgg gatggcgttc
     3061 ccgttgctct tcgcttttct aatcggaatg ttccagctag gttacggatg ttttctttac
     3121 aaccagttgc agatggtggt gcggaccggc gcgcgttacg cctcgatcgc tgacttcgat
     3181 agcgcatcga gcggcgccag cttcaggagc aacgtagcga acatggtcgt ttacggcaac
     3241 ccatccgggg gaacgcggcc gcttgtgacc gggctgagaa cttcgctggt cagtgtgacc
     3301 tggcaggcgg atggcgccgg cataccacag acggtcacgg tcaaaatttc cagctacagc
     3361 ttctcggttc tcgggaaagc gtaccagctt accaacaagc cgcaggccac attcatttat
     3421 ctgggccagt ttctgagttg aggatgccct tcccctatgc gaaattgtct ctttagcaaa
     3481 acgaatggcc gcagggacgg aagcaccatg gtcgagacgg cgctcattct ggccgcggcc
     3541 ctgattacct tcatcggaat catcgacgtc gggtccgtgc tttttcggct gcaaggcctg
     3601 gtggaacgcg cgcgcgccgg agcgcgctac gctgttgtaa attcgttcga cgcgacgaag
     3661 atccaaaacg tcgtcgtgtt tggaaattcg gcgggaaacg ggaatcccct cctcgggttg
     3721 caacccggct tcgtcacggc aaatctgatc gatctcggcg atggaaacga aaaggttcag
     3781 gtgatcatct cgggttacac cttcaacttc ttcaccccgt atatcgccgg atccaaaaca
     3841 tttcctactg tagaagcgga tctgaccaca gaaagtctcg gcgccgccaa ctgattcggc
     3901 ctttcactga aacaaccgga ttcgatacag cccgaggctg ccgccgccgt tagctccgcc
     3961 gcctgccggc agggcgctgg ccgggccgat gtactcggcg cagcatggct gcggattgct
     4021 gcccgggcag ggaaccggag gaaggaagaa ctgggcaaag cccatcacta cgtcgttggt
     4081 gggattgttg accggcaccg tgacgatgcg gcgcccgttg ccgtgtggag gattggcagg
     4141 aggctggccg ggtggatcga cggagtagta tgtccggccg ggtggatcct caccgaccgc
     4201 ggaggcaggt gcggtatagt aagtcggcgt tgtgggattc gtatcctgtg ccactcgggt
     4261 cagcaagctg gtaacttccg tctgcttgtt cccgctagcg ttgtcgatcg tctggcccac
     4321 ttgtatcgga ttcgggattt ctacattgcc caggatggcg ttacgaatga aattcgcgcc
     4381 gcctccgcca ccgccattca acagatcgat atagccccgc tgtccgttgt tgtcgctgcc
     4441 ggggataaaa ccgggcgtat tcatatcgcc cgcgcagccc accaacttat tcccgctttt
     4501 atctgtaata cctaccttgc ttcctttgcc caccggatcc cagcgcagcg tataaagctg
     4561 gcccttgatg aagccaaagt ttcctgtcgg atcggccggc agcggattgg gaacgtgggc
     4621 atcaggagag tacgggaagg cgccgtcgcc cagcccgctc agcagcgtct ggccggcgac
     4681 cgccgtcgcc ttgacggtgc tcgaggcccc gatgccggcg aatatcggca aaaagtagac
     4741 gggcacgttc ccctgcacct gaactcgggt gaaggcatag ccggttgccg ggctcgggtt
     4801 gctcaagaac gcgccgccgg gagaggtagc gaaatctacg ttcaccgaag agacggagct
     4861 gctgtcgaag taccacttat tcacgttcgt ttgtgcttcg ctaatggcgt tggcgatgcc
     4921 ggcgctggtt ccatccaacc ggttagccgc cgcgatggcc gcggcgtcag cgtagttttg
     4981 cagctcattc ttagcgatat agagccgggc caggtccaga cataggccca acatgcccaa
     5041 gatagccacc acgctgacgg cggcagtgac caagacaaaa ccacggcttg cggcgccgta
     5101 gcgatttttg aacgcagagg tcatagtcgt ccccgaactc ttatcggccg gatagctccc
     5161 ggcgataagc ctatcgtgga tcttgccggg gcgtaacggc gggtggtacc tttgtccttc
     5221 ttatccggag gattgctgag atccattctc acttggcgaa cccggccgct ttctgcgggg
     5281 cacagttctg ccgcaggctg ggcaggaggg ggatgaacag ggacaactta tgcggatacg
     5341 tccttttata ggtggttttt gtgttctttt gacggcagcc acgatcgtct cctcgcaagg
     5401 gctgcaaatc gggagagggg tcatcacaac cgcggtcggc accggagata aagatcccga
     5461 cggcaacggc ttttataacg gcgacggcaa accgggaccc cagacgcaat tgaatttcta
     5521 catcggcgcc gacgatctgg aggacttcgc tcaccttgcg ttcgactccg caggcaatct
     5581 ctactttccc gacaaaggca accagcttat ccgcaagctc gacaagaatg ggatcgtgac
     5641 tacgatcgcc ggcagccccg gcgtggatgg attcaacggc gaccaggcag atgccaaaaa
     5701 ggctacgctg agttttccca ccggcatatt cgtggacaag gcgggtaaca ttttcttcac
     5761 cgatgaggac gcaggggttc agcaaggcgg gctggttcgc aaaattgata ccactggcgc
     5821 actaaccacg gttgctggaa gcggcaagtt cggctacgac ggcgatacca agccggctac
     5881 acaggccgcc ctgaacgggc cgacagccgt tgccgtggat acggacggaa cgatctactt
     5941 taccgatcag tttaatgacc tggttcgcaa agtggatccc aaaagcggga tgattacaac
     6001 agtcgccggc aacggggggc acggcaacag ccaggcaggc gccgacggcg tgcccggtac
     6061 ttccgtgact ttgggctggc caagcggtct ggctctcgac ggcaagggcg atctttttat
     6121 cgccgatcag acaaacaacg ttatccgggt gctgaacctg caaacgggac ttatccggcg
     6181 gatcgcgggc acgggccagc acagtgacga taacccgggc ggtaacggag gtccggcgac
     6241 gcaggcggct ctcggatatc cgattggcct ggtcttggat cctgcaacgg gcgacctcta
     6301 cttcgcggac atgcacaaca atgcaattcg caaaatcacc gcgccactga ctgcgaacgc
     6361 cattatcaca accatcgccg gcaccggctt tttcggcttc gccggcgacg gcgggccggc
     6421 ctcggccgcc caactggcgc ggcctgcggg cttgaccctg gatgcaaacg gcaatttgta
     6481 tttcatggat tggtacaacc agcgcatccg caaagtgact ccgggctccg gtattccgca
     6541 accggtggtt ttttccgggg gcgtggtgag cgcgggggga ttcgccccgg cgccggcccc
     6601 ggtggcgccg ggatcgattg tggcgatttt cgggcgcaac ctcgcttccg gaatcggctt
     6661 cgcggcagct actccgctgc ccacgaccct gctcgattcg cagcctgtgt ctgtgagcgt
     6721 aacggccggc agcggcatgg tttccatgcc gctttttttc gtttcacccg accaaatcaa
     6781 cgcgcaattg ccttttgaag tggcgccggg ccaggcaact ctgaccgtaa atgtgggagg
     6841 ggtgcagagc gctccggtga cctttcaggt atcgtccaca gcgacgggta ttttcactta
     6901 ccccggaacc aaccgggccg tagccgtcaa tcaggacggt tctctaaaca gcttctccca
     6961 gccagcggcg cggggcagta ttattacggt gtacctgacc gggccgggcg acctgaaccc
     7021 gccgttcgct acgggccaag gggctccgtc caatcctctt tcccaaacca aatccagcca
     7081 cagcgccatc atcggcggga agctttcgca gatcaattat ctgggagcta cgcccggata
     7141 tgtgggcctt tcccaggcga atatcgtcgt gcctgacaat gccccggtgg gcgatcagca
     7201 agtggtgctg catgtcggcg atcaggacag cggaaaccgc ccggttgtga ctgtcaaata
     7261 atggcagggg agtgcggcct gcgctgggtt aaggactcgc ggctggatcg gagttggaat
     7321 aaagataccg gctcagtcct tccatgacgt tcacaaccgg ggcgccgcgc aataccggcg
     7381 taggcatggc ggagtcccaa cgagcgccgg gaggaaacaa ggcgaggaaa tcccagatcg
     7441 tatttccgcg cagtactgac ggtatgccgg cagccttgat tctttgcgat acgagacgat
     7501 ttctatccca gaactggact gcccgccggt cggaaattct cgccagcaca tcggaggtag
     7561 gagcgaaaaa gtcggttctt tccaccggct cccagaccac cagcacgcgc agccgcgctt
     7621 tgggaaaatc ctccagcgtc gcctggagcg cagaagcgcc ttgcagcgac cgcttctcgg
     7681 agggcgaaaa gagcaccaga atccgggtgg tttcggcgga atcgttgaac acgcgctgga
     7741 aatcggaaaa agtctgatcg gtgatcgaca cgagcgctgg ctgtccctcg ggcacgtcgc
     7801 gaggcccgaa aacgcgaaag gcaacaatga gggccagcat catcaagatc agggcggcgg
     7861 cccgccatct acttttcatg cggtggtcgg ctctcccagg gcttcataac aggggttgag
     7921 cgcgctgcca atggtcctcg aaaaggcggc gcaacgcggc agccagagca tcgtgttcaa
     7981 agaccagggc cgtgatttgc gggcgagtga gaaccggatc gacgagcgcg atcatgccga
     8041 agcggccgtc gaacaaggca agctttagcg ggagctcgct gtccacgcga atttgcgctc
     8101 catatttcac cagcgcgcga agattcttcc tgtgcacggg gttcatgtcg cggctttcaa
     8161 atatgaggcg gcactcgacg ttgcgttcga gcgcctcccg gacgcgcggt tcgctcaccg
     8221 ggtccacggc gtagggccgg cgcgagaatt ccagatattc tcgcgtgctg cgcgccagca
     8281 gccgccggta ctctccggaa atctggcccg attcgttgac gatgcgtaaa taatccaaag
     8341 gcccgcggtc ctggtggccg tcgataaaga ccgagtgcag atcctgatgg agcatttcgg
     8401 caagacggcg ccggtcgtcg agctcccgtt caaaaacttc gcggcgccgc gccagatagc
     8461 cttccagcgc cagcttgggt tcgaccgctg aaaacagctt ggctttgccg tgtactacct
     8521 gtgcgaagcc cttttccacg agactgccga gcacttcgta gattttctgc cttggaacgt
     8581 gcgcgcgcgt ggcgagctcg agggctttga agcgcggatg gccgatcaag gtcagatagg
     8641 cgcgggattc gtaagcgttc aggccgatct gctggaggcg ccggagttgt acctgcaagt
     8701 ccactgcaag agaaagatat cacaacgctg cggccgcagc ggagcggcga atcggctatg
     8761 atggtgggcg ctggctggcg gtgggtatgt tcaaggaacc catattccgc gatcgcgtcg
     8821 ccgtgatcac cggagggggg accgggctgg ggcgtgcgtt tgcgctgcgc ttcagcgaac
     8881 tgggagcgcg cgtcactctg gccagccgcg attcgtccca cctcgagccc gcccgcgcgg
     8941 agatccgccg tcgcggagga gaggccctgg ccgttcccac cgatgtccgc attcccgagc
     9001 aggtcgagaa catggtccgg gaaaccctgc gctgtttcgg ccgcctcgac atcctgatca
     9061 acaatgcggc gggcaacttt ctctgcaagg ccgagaagct cagctacaac ggatggcgtt
     9121 cggcggtgga cattgttctg aacgggagct tctattgctc gcgcgccgcg ctggaaccga
     9181 tgaaaatcca gagatacggc cgcattttga acattctggc cacgtatgca accggcgcca
     9241 gtcccggaac cgtccattcc tgtgcggcca aagcgggagt gttggccatg acgcggactc
     9301 tggcagttga gtgggcgcgg tacaatgtgc atgtaaacgc cgtggcccca ggtatgttcc
     9361 caaccgaggg tgcgagttcc cgcctgatgc cggccccggg ggctggcgaa cgcgccctcc
     9421 agagaatccc cgccggccgg gcgggacgtc acgaagagct ggccaatctg gcggcctacc
     9481 tctgctcgga ttacgcggac tacatcaacg gggaatccgt cgccatcgac ggaggagcaa
     9541 gctggaatca ccccagcttt gaatgggaga acctgtgaaa ccgtcttctt accacttacc
     9601 agcaacattg ggcgaactgc gccagagcga atttagccag gagcggatcc aaaaccgcac
     9661 cgtaaaggat gagatgcggg ccaatctgat ctgccgcatg gaaaggggcg aacccctgtt
     9721 tccgggaata atcgggtatg acgacacggt gatcccgcaa gtagtgaacg cgattctctc
     9781 ccgccaccac ttcatcctgc tcgggctgcg cgggcaggcc aagacgcgcc tgctgcgcgc
     9841 tctcaccgaa ctgctcgacc cggtgctgcc gtacctcgcc ggatgcgaga tccgtgacaa
     9901 tccgtacgcg ccgatctgcc agcactgccg cctgctggtg gcggaaaagg gcgatgcggc
     9961 gccgatcgcc tatcttcctc cagagctgcg atacgtcgag aagctcgcca cgcccgatgt
    10021 gacgatcgcc gacatgatcg gggatatcga tccgatcaag gcggcgcgag ggggcttgga
    10081 tctttcgagc gagctgacta tacactacgg cctgctgccg cgagcccacc ggggcatttt
    10141 cgccatcaac gagctgcccg atctggcggg caaaatccag gttggtttgt tcaacatcat
    10201 gcaggaaggc gacatacaga taaaaggcta tccggtgcgc ctgccgctgg acgtgctgat
    10261 ggtctttacg gcgaacccgg aggattacac ggcccggggc aagatcatca cgccgcttaa
    10321 agaccgcatc ggcagcgaaa tccgcacgca ttatccggta acgctcgatc acggcatcag
    10381 catcaccgag caagaggcgt ggaccgaccg gcggccgcgc ggcggcatat gtattcctca
    10441 gtacataaag gagatcgtcg agtccatcgc ctttctcgcg cgggaggaga agaagatcga
    10501 caagcgctcg ggcgtctccc agcgcctctc tatcacctgc ctggagaacg tcgtttccaa
    10561 cgccgagcgg cgcgctctgc gcaacaagga gcggctggtg gtgccccgcg tgagcgacat
    10621 ttacgcggcc ctgccctcgc tgaccggaaa gttcgagctc gagtatgagg gtgagctgaa
    10681 gggcgccgat gccgtcgccc gcgagctgat caaggcggcg gtcggccgca tttacgaccg
    10741 ctattttgat ggcgtaaatg tttcccagat agtccagtgg ttcgaactgg gcggaacctt
    10801 gaagatccgg gacgcgcagc ccgcggcgga gctgctgaaa caactggaag gcatccaggg
    10861 cctgatggaa gcggtcaaga cgctggagac ggggctcccg aaggatcccg ccgtgaaagc
    10921 ttcagcggcg gaattcgtct tggacggcct ctatgcgcac cggcgcatca gccgcagcga
    10981 ggagctcggt tacatggcgg aggaaaagaa gcgagagccg cgcgaccacg acgaattccc
    11041 gccgcgcggg ttccggaaac actataactg aagcctggcg gcggcgtcat gaaatttacc
    11101 acctactcca aatacaccgg agacgacctg ggcctgagcg ccgaggacct gctgcaagcg
    11161 ctcgccgact tcctccttcg cagcggcttt gatcagcagt acatgcactt ccacgaaatg
    11221 ggcgagcaga cgctcgagga tctgaaacag gccattcagg aggcgctcga gaatggaagc
    11281 ctgttcaaca acgagaacat gcgcgagctg atggaacagc tccagcagat gtcggccgaa
    11341 caaatggaga agctgctgga caggctgatc gagaaaatgg ccgaggaagg ctacatttct
    11401 atcggcgagc ccaatccgca gggcgagcgg aacgtcaaat tcaacccgga aggcggcgtc
    11461 ggcgagaacc gcgaggggcg cgtgcggttc gagttgaccg acaaggctct cgactttctc
    11521 ggcttcaaga ctctgaagga cctgctcgct tcgctcggaa aggcgagctt cgggcggcat
    11581 gacacgcgcg acctggccac gggcatcgaa accaccggcg cgtcgaagcc ttacgaattc
    11641 ggcgacactc tcaatctgga tattagcaat actttattta acgctatcaa gcgcgagggc
    11701 ctgcgcgtgc cgctgccgct cgattatccg gacttgatgg tgcatcaatc ggaataccag
    11761 agctcgtgcg ccacagtgct catgctcgat tgcagccaca gcatgattct ttacggcgaa
    11821 gaccggttca ctccggcgaa aaaagtggcg ctggcccttt cccatctgat ccgggtgcag
    11881 taccccggcg acagcctgca cctggtgctg tttcacgact ctgccgagga agttcccttg
    11941 tcccagcttg cgcgggtgca ggtcgggcct ttctatacca acacgcgtga ggggctgatc
    12001 gtggcccagc gcatcctcgc ccgccagcac aaggacatgc ggcagatcat catgatcacc
    12061 gacggcaaac cctcggcgct cacgctcgac gacgggcgga tctataaaaa cgcgttcggc
    12121 ctcgacccgc tggtcgtggg ccggaccctg gaagaagtgc acaaatgcaa gcggcaggga
    12181 attttgatca acacgttcat gctggccagc gactacgggc tggtccagtt tgtccagaaa
    12241 gtgaccgaga tctgccgagg caaggcttac ttcacaactc cgtacaccct gggacaatac
    12301 ctcctgatgg attacatgaa ccgcaagacg cggacgatcc attgagcagc attgctccgg
    12361 gagggaacgg ccgcgcggcc gcttcccggg ccgagtcagg ccgcagccaa ccggatctga
    12421 gccagccgtg aatttcgcaa atttagctcg gcctgcctcc gcccgccggg gttcgtgaaa
    12481 gatgtcaaaa cggctgactg cgcgctccct tccgttccgc tttctccgca gacttgtacg
    12541 ctacactgat agacgaggag gctcgatgcc gacggttaaa gcaggagtcc aggcgcccga
    12601 cttcacactg ccggatttga cggggcggcc ccaggccctg agcgccttgc tcgcttccgg
    12661 cgatgttttg attgcatttt tcaaggtctc ttgccctact tgccagtaca catttccctt
    12721 tttggagcgc ttgcacaagc aatcggaggc acggcgtttg cgagtgtacg gaatttccca
    12781 ggacgaccgc cagaagagcg caaagttcgc tcaacaattc ggcgtcagct ttccgatttt
    12841 gctggacggc gaagaggaag attatccggt ttcgaatgct tacggcatta cgcacgtgcc
    12901 ctcgcttttt ctgatcgatc ccggcggccg gatcgtcctg accagcgtcg ggttttgcaa
    12961 agcggacctc gaggagatgg cgcaacgcgg cggcacgttg cctctgtttc accgcgacga
    13021 aaaaatcgaa gcgtttcgcg ccggctgagg gtcgaaaaac tgagctccgg gacggagttt
    13081 cccatcatga aaaagcagcc ggcgcggcag cgcaaccgct ttcgcgccgc gaaagaagtt
    13141 cggaagctgg ccagggaacg aatcggagcg gtgccgcccg gccgggtgat cgagccgaag
    13201 ccgcggcgca aggcggccaa gcatcccctg aaggaactgg aacgagaact ggaagcttga
    13261 agaaacggct gccgcaggca gcgcctacgc gccgctcgaa gcaccctcgc gcagccgtac
    13321 gattcgctcc tgacagtagc ggcgctcgaa ttccgcgcga gtggcgaagc tattctggtc
    13381 aatagcgcgg tcgatggcca gaatcccgtc caggtgatcc agctcatgct ggaggagctc
    13441 ggcgaaatcc cattcggcgg cgagcgagca agccacgccg cgatgatcgg cgtagtcgat
    13501 ttgtacacgc tcggcccgga cgagccggac catgatctgg gggaaggaaa agcagtcgtc
    13561 ccatacgagg attttcgatt cgccggcgcg gcgtagcacc gggttgatga gcgagtacga
    13621 gcttccggcg atttcgagat atattaatct ttccggcatt ccgatttgaa cagcgctgat
    13681 gccgcgcccg aagccgtacc gctgctgaaa atcgtggagg gtgtcgcgca ggtcttgcaa
    13741 agcggcgagc gcggcctgga agttcactac cggccgcgag cgctcgcgca gcgccggatt
    13801 gccaagctgg aggatctcgc gaacggccat ggatttagca tatcagcgcg gtgcgcggcg
    13861 agcgccgctt ccgcattccc agattctccc gcacggcggc gcaggttcag gccaggcctc
    13921 cttggtcctg cgcccggcgc cttgagcggg cggccgcgag cgatgccatg aacgttttcc
    13981 aggggcatac gggatggctg gaagtgatct gcgggccgat gttttcgggc aaaagcgaag
    14041 agctgatccg ccgcatgcgg cgcggcatga tcgcccgtaa gcgggttcag gtattcaagc
    14101 ccgccattga caatcgctac tcgagcgacg agatcgtttc gcatagcgac ctgcgcatac
    14161 cttctcaaac cgtcaactcg gcgcgcgaga ttttggagct tctggagatc cggtccgaac
    14221 tgatcggaat cgatgaagca aactttctcg gcccagaact gccggaggtg gcaagccggc
    14281 tggcggacat gggcaaacaa gtgattgtag ccggactcga tacggactac ctgggccgcc
    14341 cttttgagcc gattccctcc ctgcttgccc tggccgaaga gatcaccaaa acgctggcca
    14401 tctgtatgca gtgcggcgga ccggcgaaat tcacccagcg cttggtggca tccgatgatc
    14461 tgatcgtggt cggcgcggcg ggcctctacg aagcccgctg ccggcgctgt ttcgaggcag
    14521 gctctccccg gcagcacgcg ctcgatttcg tcaaaccggg atcgggaaag gtttgaacgg
    14581 tccggtcttc ccatgccttc catgttcccg gtgactcccg agccagagca gtcgcctccg
    14641 gcgaagacgc cctttctcga gggccgcatc ggccgttcgc tgatccggct cgccatcttt
    14701 ctggttttca cattcgctct ttggtggctg gtcgcgggct tgatggcgcc atttgccggc
    14761 ccgctggtaa cgagcgctat ttcactgggg gctacggcgc tcgtcacgac agccgtcatg
    14821 atgcgcattt acgaaatgca gccgttatcg gccgtgggcc tgttctggaa tcgcctgggc
    14881 gggatccatt tggctctggg cctggggctg ggaagcggga tgtcgctgtt gatcacggcg
    14941 attcagtgga gctgcggctg ggctcgcttc cagcgagagc cgctgccgcc cgatgccggc
    15001 ctcaccatcc cattctggat ttttgttcta gccatcggcg cggcaggcga agaactgctt
    15061 ttccggggct acgcgtttca gaatctcatc cgggcgtttg gtccctggtt ttccgtaatt
    15121 tcaacatctg ttttgttcgg tcttgggcac agcgccaatc cggccttttc gagggtgagc
    15181 ctgctcaaca cggcgctgtt tggactctta ttcggctacg cctactggcg cacacgcgat
    15241 ctctggctcc ccctgggcat gcattttgca tggaatttta ctctcgccgc tatcggcgcc
    15301 aacatcagtg ggcttaaaat tagactgatg gggctgtcgg tgagctccgc aggctcctgc
    15361 ttgtggagcg gcggtgatta cggccccgag gcgagcctga taaccaccct ggttctgaca
    15421 gccgtggtta tctttctatg gaaagcacca accgcccggc aggaggacgg aatcctggcc
    15481 gggctgccat gaggacgccg atgaaagctt tttgggctgg cttactgatc ctgggactca
    15541 gcgccgcgac aggcgaggca aagttgacct tcgaggagcg caccgaactg gtgcgcgggc
    15601 tgatggccga attcgccact gccaaggttg tgctcccgcg gtcgaaaaag ccgctggaag
    15661 tcactccggc gggcaagtat gacaaaaacg tgtgggccga ggcgatgcag gaaaatggac
    15721 cggcggcgcg agtgggcgat atggtgcaga tcacgaaagt gaccattgaa aacgaccgca
    15781 tcgtcctcga gatcaacaat ggaatcaagg ggaagcatcg ctggtggcac aatgtccaga
    15841 taaccggagc caccggcaat acgtcgccga tcgtcgccga ccagaacacc aatgcgccca
    15901 gcgggactac agttgcgctg gttttcgggg atctctcgaa gccgccgaag gcagcggaca
    15961 tcaagaagat gcttcagccg gtgatggatt ttgacaagca tacagccacc gagctataca
    16021 cgcagactct tccgaaagag atccaggatg cgatcaaagc aaagaaggtt ctggtaggca
    16081 tggaccggga gatggtgctg ctggccaggg gacatcccga ccgcaagctg cgggagaaca
    16141 aggacggggt ggaaacggaa gactggattt acggcaaagc gcccggcaca atcacgttcg
    16201 tgacctttca gggcaacaac gtgatccggg tgaaagatga ctacgcggcg ccggagtagg
    16261 gcgggaactc atacctgcca atttcaaaat acggaggcag aatggcagca gtgaaagtag
    16321 ggatcaacgg ctttggccgg atcgggcgca acatctatcg cgccgccatg caccgggagg
    16381 gtatcgacat agtcgccgtc aacgatatca ccgataccaa gactctggcg tacctgttga
    16441 agtatgactc agtgctgggc aacctggagg cggaggtgaa agccgccggc gagtgcatcc
    16501 aggtggacgg ccgccagttg cgggttatgg cccagagaga cccggcgcag atcgactggc
    16561 cgtctacggg cgcccagatc gtagtggagt ccacaggact tttcaccaac gccgcagacg
    16621 cccgcaagca cctgcggggc acggtaaaaa aagtcatcat ctcggccccg gcaaaaggcg
    16681 aagatcttac catcgtgctc ggcgtcaacg acaacaagta cgatccggcg cggcactcga
    16741 ttctttccaa cgcttcctgc acgaccaact gcctggctcc ggtagccaaa gtgctccacg
    16801 acagctttgg cgtcgccaag gggacgatga ccacgattca cagctacacg aacgatcagc
    16861 aaatcctcga ttatccgcac aaggacctgc gccgcgcccg cgccgccgct ttgaacatga
    16921 tcccgacgaa aacgggcgct gccagcgcga tcggcttggt gatccccgag cttaacggca
    16981 agctggacgg ttttgccatg cgcgtgccga cgcccgacgt ctccgtagtc gatctggtgg
    17041 tcgtaaccga gcggccggtc acggaaaagg aagtgaatca gaggctgcaa gaagcggctg
    17101 aaggttcgat gcggggcatc ctgggttaca ccgaagatcc tgttgtttcc accgacttcc
    17161 tgcacgatcc ccgcagctcg attgtggatg gcgccatgac caaagtattg gacgggaatc
    17221 tggttaaggt gctggcgtgg tatgacaacg aatggggctt ctcctgccgg atgtgcgacc
    17281 tgatcgaaat gctcgccaac cggggactgt aggtggccct ttccctgcga gatctcgaac
    17341 cgctgctgcc gggcaagcgc gtcttcctgc gggtggattt caacgtgccg ctcgacgaga
    17401 gtgggacccg catcagcagc gacaagcgca ttcgcgcttc cctgcccagc atccgttacg
    17461 tgctcgaaca caaagcagcg ctgtttttgg cctcccacct tggccggccc aagggcaagc
    17521 cgaatcctag gatgagcctg aaaccggttg cccagcggct ctcggagctg ctgggccagc
    17581 cggtgacttt ggccccggat tgcataggcc ccgcggtgcc ggcgccagcc ccggggcaag
    17641 ttgtgctcct ggagaatctg cgcttccatc ccgaggaaga aagcaacgat ccggagtttt
    17701 cccgccaatt ggcctcgctg tgcgatgttt acgtgaatga cgctttcggg tcggcgcacc
    17761 gcgcccatgc atcgacggcc ggcatggtgg cttttgtccg ccacgcagcc gccgggttcc
    17821 ttatggaaaa ggaattgaaa tacctctcca tggcggtgtc caatcccagc cgtccgtacg
    17881 tggccattct gggcggggcc aaggtctccg acaaaatcga agtcatccaa aacctgctga
    17941 agcttgtgga ccggctgctg atcggcggcg gcatggccta cactttccta aaatcgcagg
    18001 ggctggccgt gggccgatcg ctggtcgagg aggacaagct ggatctggcg cacgagcttc
    18061 tgcgaagcgc cggcgacaaa ctgcggctgc cttccgatca tgtggtggcg gccgaattga
    18121 aagctgaggc gctctcgaag gtcgtggatg gggcaagggc gccgattccg gattcttgga
    18181 tggccgtgga catcggcccg gaaacgatca ccgcctacag ccgggaggcg gctcgggcca
    18241 gcctgatcgt gtggaacggc cccatgggcg ttttcgaaat agcgcctttc gccaagggca
    18301 cggtggaact ggcgaaagcc atcgccactt caaaagccac gagcatcgtg ggcggcgggg
    18361 attcggaaaa agcaatccag gcggccggcg tcgccgacaa gatcacccac atttccaccg
    18421 gcggcggagc taccttggaa tttctggccg gcgaaaccct gcccggcgtc gcagcgctcg
    18481 aaaaaaaggc ttgagcgtca gagcgccggc tttttccgcc gcagcggctt aaagcggtac
    18541 ttcaaaatgc accacagcgc gcgcaggccg tctttgaagc cgattttctt cccctcttca
    18601 tacgtgcgtc cgtggtaggc gatgccgacc tcgtagattc gagcgcgcgc cctggccagc
    18661 ttacacgtga tctcgggctc gaagccgaag cggtcttcct caagctcgat ggattggatt
    18721 aattcgcggc ggaaaacctt gtagcacgtc tccatgtcgc tgagattcag atcggtcgcc
    18781 atgttggaga gcagggtcag cagcttgttt cccatcgagt gccagaaata gagcacccgg
    18841 tgctcctcgg tggtgatgaa acgggagcca tagacggcgt cggcttgctg ccgctccagc
    18901 gggccgagca gtttgtggta gtcctccgga tcgtattcga gatcggcgtc ctgcacaatg
    18961 acgtattcgc cggcggccgc cgcaaatccg gcgcgcaagg ccttgccctt tcccatgtta
    19021 cgcggttgaa atatcacatg aacattgccc tgctccgact ggatctgttg cagccgctcc
    19081 cgcgtgccat cggtggagcc gtcgtcgaca ataatgatct ccttggaaat gttgacgcgc
    19141 tggacgcggc tgacaatctc gccgatcgtg tctttctcgt tgtagaccgg gatcaccacc
    19201 gaaagcttca cgggattcat tttgttgcca aaacaataga actgatagtt gtatcagctt
    19261 ccggggaaag ccgtcaaaaa tagatcgagg ggcgcgagcg ggctccatac gaaatattca
    19321 gcgcaactcg gcccgatgca gcttcagcca gccgagcatc tgccggaagg ccttcccgcg
    19381 atggctgtgg gccagctttt cccgcgggct caactcggcg aaagtgcgtc ccaggggcgg
    19441 gaaataaaac agagggtcgt agccgaaccc gcccgacccg cgcggggtat ccacgatctc
    19501 gccttcggcc gttcccgtga acgtggccag aagccggccc ggcaaagcga gtgcgattac
    19561 acagacaaac cgggctgcgg gccgcggcgc gccgcacccc ggagaaagat gcagcctttc
    19621 gaggagccgc ctgcggttct cttcgtcgct tgcgcccgag cctgcatagc gggccgagtt
    19681 cacaccaggc tcgcctccta attggtccac ttccagcccg gaatcgtcgg aaaacaccgg
    19741 agcgccgatg tgcccggcat agcatagagc tttttgaatt gcattttcct cgaagctgct
    19801 tcccgtttcg gggcaatcac aatcgcccgc cggcacgatg cgccactcag ggggcgccgc
    19861 cagctcgtat tccgcgagct tgtgggcatt ggaggttgcg cagcggatta ccatgagacc
    19921 acctggcgtt ccgccccgat cagatcctga atgcccttgg cggccagggc aagcataacc
    19981 tgcaactgcc gctcgtcgaa gagcgcatgc tcccctgtgg cctggacttc cacgaagcgg
    20041 cctccgccgg tcatgaccac gttcatatcc acgcccgcct tggaatcctc ttcgtaacaa
    20101 agatcgagcg tcggaacgcc atccaggagg ccgacgctgg tggcggccac gtagtcgcgc
    20161 agcggtatgc gagacagaat tttcgagtcc accagttgct tcaaggccag cgccagcgcc
    20221 acgaacgctc ccgtgatgga cgctgtgcga gtgccgccgt cggcctggag cacatcgcaa
    20281 tccacaatga cggtcctttc gcccaacagc gcgagatccg ccacggctcg cagcgaacgg
    20341 cctatcaagc gctggatctc ctgagtacgg cccgataatc ttcccttcac cgattcccgc
    20401 ggcgtccggg tcaccgtggc gcggggcagc atggaatatt cggcggtaat ccagccggtt
    20461 cccgagccgc gcaagaaatt gggaaccatg tcctccagcg aggcggtgca aaggactcgc
    20521 gtgttgccca cttcaatcaa cgccgacccc tccgccgtaa ggaggtagtg cggggttatt
    20581 cgaacaggcc gggcctgatc cggaagacgt ttatcgctgc gcatagttgg ggagaaaaac
    20641 gctatcccac cagattgcgc ccggccgctg ccggaaaaac gaagtcgatg cggcagccgg
    20701 aggcgctagc tcgatttgat catcgcataa aacagccaca tcacaaacgc catcgccact
    20761 accagccaga agatgcagcc aatctggcgc gcccggtttg attccggctt cttgcgggtt
    20821 cccgcgggtc tccatttggc cataaatcga gtgacaggaa agcggcaagg acgccgttac
    20881 cggggagacc gcacccggct cctttctgca atccgccgcc aaagacgact ataaagtccc
    20941 ttataccgca ttcccggcca gccgttccgg ccggcgtccc caatcggtgt ccagacagta
    21001 gcgtacaacc ttgtcgtagt aactgcgaat gggagaaaga gccaggccgc tgccttggag
    21061 cgccgcggcg gtgttgcgat tctcaaacgc ctgaaaaagg ccgaggtggg gcaaaaagta
    21121 tcccaagaca cggagaagct cgttgagcag aacgtcgccg ctccgtctgg tctgctcgac
    21181 aaatctctga tactcagcca gagttaccag cttgggcagg cggatgcggc cgttgcggcg
    21241 gcaggccggg tgactctcca tgagatcgac cgtaagatcg atcatctggc tcaccgtcag
    21301 gctggcatcg ggaccggcgc aaagttgata tacccggcct ggaacaaatc ggttctcgaa
    21361 caggtaggcc agcgccgcga cggcccagtc ggatgatatt aaatccaccg gcgcctgtgg
    21421 atcgcccggc gccagaggca atacattgcg cggcagcaat cgcaagagtt gatgaacatg
    21481 attgaactgg cggacgcgtc cggtagtgga gtcgccaatg atggaactca gccggaagat
    21541 gactgccggc acctcgccca tgaagcggaa caccagctcc tcggcctcat acttcgattg
    21601 ctggtacgtg ttgaggaatc cgttgtgcgc agacagccgc tcctcggcga tgaccccgct
    21661 gcggcgcccg gccacataga ccgtgctgat gtgggcgaac ttttggagac gtcggcagcg
    21721 gcgcgccagc tccagcaagt gcagcgttgg cgttacattg gccgcgcggg cctgttcgac
    21781 aggcaggccg aagcggatat cggcggccgt atgaatcatc tcggtcaaag agcgctccag
    21841 atcggcgagc agagcgctat ccaggcccaa gctgggctgc gcgaggtcag ccgcaacaac
    21901 cgttacgttt ggattctgat tcaggcgctc cgccccggcc ggccggcgcg tgagcgcgat
    21961 gatacggcgc tgcggatcgg cggcgagcag ccgctccaga agttcaccgc cgaccaagcc
    22021 ggtggcgccg gtcaaaagca aagtggggct ggcagaattg atcaagccgc ctcaggccgg
    22081 accgccgcgc cctgaggttg cgtgtttcct cggcgcttgc ggtcaaagag catcttcctc
    22141 acaggagtgc aaattatacc agcaaacgcg cgtcctgacg ccccgctcaa gccggcgatg
    22201 aagcctttcc ggggttttgg gcgctgctgt tttccggggc gcgcggatcc atcatctcgc
    22261 gcgccgtaac gaagcaatag ccgaggcccg caagctccgg aaggatccgg cgcagcgcgc
    22321 ggatcgtatt gcggcggtct acgcgctcgg agacgcgatc gccgtcgtgc atgcagaaga
    22381 tcgcgccgtt cgacgcatga cttatcacat gccaggcgat ctcgtcggcc tcccattccc
    22441 aatcataagc gatcaccgtc cacatcacgc ccgtcaatcc gaactggcgg aggacagggc
    22501 ggaggccgaa ccagcgaatt ccatacggag ctcggaacag gcggggcgcc gttccggctg
    22561 cccggctgat cgcctgctgg gttctgccga tctcctcgcg gatccgcgac ggcgtgcagc
    22621 cgagcagacg cgggtgggaa tacgtgtgat tgccgatttc gtggcctccg tcttggacgc
    22681 gccgcgacac ttccggcaag cgttctacgt ttttgccgca ctgaaagaaa gtagcgcggg
    22741 ccgagtaaag ctccagcagc tctaaaatcg gcagagtggc ttcgctcggg ccatcgtcga
    22801 aggtcagcgc gatttgtctt ttttcgcgcg ggccgcgcca gaggttggga ccaaaaagcg
    22861 gcgaggagcg tccccgaacg ccgtagcctg ccgccagcgc cgccgtcagc ggcgctccga
    22921 ttcctagctc gagcaccatt ttgttaccat catagcctag agtgcctatc tcgttggaaa
    22981 actcagaaat cgcggcgttg ctttacgaaa ccgccgactt gatggagatc gcaggcgagg
    23041 atcctttccg catccgcagc taccggaatg gcgcgagcgc gatcgagacc tgccccgagc
    23101 ggatcgccga catcgtaacc aacccggagc gcgacgtcac cgagatccgg ggaatcggca
    23161 aggggctggc ttccgtcctg aaggaaatcg ttgaaaaggg atccttcgag cggcgcgatc
    23221 agctgttgca aaagtatccc gccggcgcgc tggaactcct gaagatccag ggcctggggc
    23281 ccaagggcat cgctctgctc tactcccact accaggtcag caatatcgac gacctcgagc
    23341 gagtgtgccg cgaaggcaag ctgcgggaac tgccccgcat gggcgagaag ctcgagcaaa
    23401 aaattttgcg ctcgatcgag cagtaccgcc gcagcgcttc ccgttttctg atcgatcacg
    23461 ccgaccgggt ggctggtgaa atagcggcgt atctggccga gacgccgggg gtcgagaagg
    23521 tcacgccggc cggcagcctg cggcggcgga aagagacgat cggcgatata gacttgctcg
    23581 tcacgggacc cgacccggcg ccggcgctgg agaagttcat caagtttccg aaaattcgag
    23641 aggtcctggc caaaggggag aacaagacca gcgccaaaat cggccacgaa gggattcaag
    23701 tcgacgtgcg cgttctggac cgctccagtt acggctctgc cttgcaatat ttcaccggca
    23761 gcaaggacca caatgtggcg attcgcacgc gggccattaa aatgggcttc aagctcagcg
    23821 aatacggcct gtttcgggca accgacgaag taaaggtcgc cggatctacc gaggaggaag
    23881 tgtattgcac attgggtctg gcctggattc cgccggagct gcgggaaaac acgggcgaga
    23941 ttgaagcggc ggcggagggc cggctgcccg ccctagtgga actcccccaa atccggggcg
    24001 acgttcacgc ccataccacc gccaccgacg ggcgcaactc catcgcggag atggcggccg
    24061 ccgcccaggc cgccggatac cagtatctgg ccattacgga tcattcgaaa gctctggcga
    24121 tggccaacgg cctcgatgaa aaacggctgg tggaacagat tgcggaaatc cgcagggtgg
    24181 ccctcgattt cccgggcctg acgcttctgg cgggctgcga gtgcgacatc cgccaggacg
    24241 gcgcgatgga tcttgatgac gaagcgctgg cgcagcttga catcgttatt gccagcgtgc
    24301 acagccatat gaatctggat gcgggggcca tgacggaaag actgctgcgt gctatggaaa
    24361 acccccacgt caacatcatt ggacacccca cggggcggct tcttctgcga agggagccgt
    24421 ttcctttcga ttttgagcgg gtggcggcgg aggcggccag gcgcaacgtc catttcgaga
    24481 tcaacgccag cccggagcgg ctggacctct ccgccaatct gctgcggatc gccaagggca
    24541 aaggagtaaa gttcgtcatt tccacagacg cccaccacac ccggcacctt gccaatatga
    24601 agtacggcgt gtggatggcc cggcgcggct ggctgggacc ggaggacgtg ctcaatacgc
    24661 tggacgcccg gcaatttcgc gccgcgatgg ttaagagacg ttgaacgaga cgaagcgcca
    24721 gcgcctcagg cttccgcgcg gttctaggcg cgaattcgcg tccgacagcc cgcaatccgt
    24781 tttgatggag gacgccgtgg tagaactgca atatccgatt ggaaggttcc aatttgaagg
    24841 cgacgtaacc gccgcgcaga ggcgcgactg gatcgcccaa atcgcggccg ctcccggcca
    24901 gcttcgggag gcggtccgag gtctctctgc gcaacaactg gacacgccct acaggcccgg
    24961 cggctggacg cttcgccaag tggtacacca cctgcccgac agccacctga acagctattc
    25021 ccggttccgg ctggcgttga ctgaggataa cccgacgatc aagccttacg atcagtcgcg
    25081 ctgggcggcc ctcgaggacg cccggtcagc gccggtcgag gcctcgctgc gcttgttcca
    25141 ggggctgcat gagcgctggg tattgttgct gcaatccatg tccgaggcgg atttcgcgcg
    25201 cacgtatgta cacccggaac gcggagtggt ccacctcggc cgcgaagtcg gcctctatgc
    25261 ctggcatggc cggcatcacg tcgcccacat cacctctctg cgggagcgcc tgggatggcg
    25321 gtaaagcgag cggggctctt cgcgatccgg tcttcccggg aaatcctgcg ggcgcgcgtc
    25381 ttcaccgtcc gccgggaaag agccgtcgag ccgggcggca tcgccgtgga aagagagatt
    25441 gtccaccaca gcggctcagc cgtcatgctt gcacgggatg aacaaggccg tattctcctg
    25501 gtgcggcagt tccggctgcc tgcgcggcgg aagttgtggg aattgcccgc cggccgcttg
    25561 aacgacggag aaacgccgct gcgcgccgcc cagcgcgaac ttgaggaaga aaccggatac
    25621 catgcccaaa gttggaaacg gctggttgct ttctacccca gccccggcta ttgcaccgaa
    25681 tacatgaccg tctatctggc ggaagggttg acgccgggag cgacccaccc ggatgaagac
    25741 gagtcgatcg agacgcggtg gtttacaccg gagcagatcc ggcaaatgat cgcccggggg
    25801 agaatcgagg acggcaagac gctggccggc gtcctgtttc tttttgcctt tcaaaaatag
    25861 tagcagggct gtctgaggcg caaacgggga ttgttgggca ggtcaatgga ttgagcacca
    25921 cggatccatc gcgacgaaat gatggaaagg acgtaggcgg gaagagccct gctactggtc
    25981 gcgacttatt tgagacaaca cgtacattca taatcgggcg cgccgcggcc ggcggcgcgc
    26041 ccaaa
//
LOCUS       DASREK010000299        14141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_144579, whole genome shotgun sequence.
ACCESSION   DASREK010000299 DASREK010000000
VERSION     DASREK010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 14141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14141
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144579"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1123)
                     /locus_tag="VFA54_16240"
     CDS             complement(212..1123)
                     /locus_tag="VFA54_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZS52421.1"
                     /translation="MVLPFKLVYSERYDLNLGRHVFPSQKFRLVRDRLLREGLAAPED
                     FLEPAPASDEDILLVHEPGYVQRLKTGTLSYQEIARMEIPYSRPLVEAVWLATGGSIL
                     AARRALIDRIAVNLSGGFHHAFPGHGEGFCAIHDVAVATRRLLKDQVIERALVIDCDV
                     HHGNGTAAIFAGDPAVFTISIHQYNNYPAEKPPSDIDIHLEDRTGDAEYLAALSAKYI
                     PAAARFRPQLVFYIAGADPYCRDQLGGLSLSLEGLKRRDRLVIDAALRTGAGVAIVLA
                     GGYAFDVNETVLIHCNTVAAAKEALGG"
     gene            complement(1271..2662)
                     /locus_tag="VFA54_16245"
     CDS             complement(1271..2662)
                     /locus_tag="VFA54_16245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HZS52422.1"
                     /translation="MSEIYYDALVIGSGQAGTPLAMALARAGSKTALIERRHVGGTCV
                     NEGCTPTKTMIASARVADLARRAADYGVRTASAAVDMRRVRDRKRGIVDSFRKSSQQR
                     LEQTPGLDLIFGEARFTGPKRIEVRLNSGGIQRMAAAKIFINTGTRASLPAVEGITEV
                     PALDNVSIMELETLPERLLVLGGGYIGVEFGQMFRRFGSQVTMIQSGDRLLAREDPDV
                     ADEVAKILREDGIEVLLAAQTFRASLAGSSIQLRVRTTVGERTFTGSHLLVATGRRPN
                     TEALDLPAAGIATDKKGFIQTNSRLETTAESVWALGDVKGGPAFTHISYDDYRVIREN
                     LIENGNATTDGRLVPYTVFTDPQLGRVGLSEVEARAQGREIRIAKLPMSSVARALETG
                     ESRGFMKAVVDARSAQILGAAVLGTEGGEIMAVLEVAMMGKLPYTALRDGIFAHPTLA
                     ESLNNLFTAMEGA"
     gene            complement(2659..4362)
                     /locus_tag="VFA54_16250"
     CDS             complement(2659..4362)
                     /locus_tag="VFA54_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52423.1"
                     /translation="MKSAARILALLALLSAGDRSGFAADPASRVALLYAQQRWQEVVD
                     AVPPGSEHSADLDYYRGMALARLERWADAEDAFKTGRRKAPRDKRFPLELAGVAFKRK
                     DLSRAREEIQTALRLDPEDAYANDFLATLYVLDENLPAALKYWNRIQKPHIENLRIEP
                     QPQVNPVLLDRAFAFSPASLLRGEDFWTTEARLRELSIFPRFRFELLPADGSNFDLLF
                     RPVERNGWGDGALGGLLSLLRGTPYQTITPEFYNLQRSAVNITSLFRWDAQKRRVLAM
                     FSAPLHGKPAWRYQAYVDGRKENWDLSRTFHLSATPLSGLNLQRIEAGAGIRSAVNGK
                     FAWQAGVNLIDREFRSAVLPPSLLSPVFTGGLSLGYRAGVDYNLLRLSEHRLTLDSTG
                     SWEFGRLFAPSFHAFSKLQGSVDFHWYPRPRGEDYEMTAHYRAGKTFGPVPFDELFIL
                     GIERDNDLWLHGHIGTQDGKKGSAPLGRDYLLLNWEIDKALYVNSLVTVKAGPLLDGG
                     RIYGESQGFGSERWLVDPGAQCKIGVLGAVRLVISYGKDLRSGRNAFYTMVTPAGGQK
                     K"
     gene            complement(4359..4895)
                     /locus_tag="VFA54_16255"
     CDS             complement(4359..4895)
                     /locus_tag="VFA54_16255"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HZS52424.1"
                     /translation="MVRLAIAAVAALALGFASSGIWLDVPYVKQERNGCGSAVIAMVA
                     QYWAHNGAQVPPEATDAGCIQKKLYTQHQAGIRASDMERYFQANGFRTFVFRGSWEDF
                     KPRLSKGQPLIVCLKENGAGNPLHYVVVVGFDWDQNLILVNDPAQRKLLKMPRSAFEK
                     QWGGAQNWTLLAIPQQTQ"
     gene            complement(4901..5401)
                     /locus_tag="VFA54_16260"
     CDS             complement(4901..5401)
                     /locus_tag="VFA54_16260"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HZS52425.1"
                     /translation="MILFGRIFAVAYLEVGLPAELPRAGLPPGTGGFVAGHLTHIHRV
                     LLFLMLAPLCFPAAQTEDHVVGPAGLQQALQAKMRERQLDIAKLERFFSLPPVQEAMK
                     AGGIDSTKVQQAIPQLTDEELASISMRAEQIQRDFEAGFLGIGSLTLLLLLLIALIII
                     IVAVKA"
     gene            complement(5445..5849)
                     /locus_tag="VFA54_16265"
     CDS             complement(5445..5849)
                     /locus_tag="VFA54_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52426.1"
                     /translation="MRLYAKTTPLIVFVLTALLVLPRDVYAEDHVVSPADLQKQMEAV
                     SDARQKNLAKMDHFFSIPRVKKALESSGLAYSAIQKAIPQLSDDELARLASRAEKAQR
                     DFEAGALTNQEITYIIIALATAVIILVIVEAK"
     gene            complement(6244..6771)
                     /locus_tag="VFA54_16270"
     CDS             complement(6244..6771)
                     /locus_tag="VFA54_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZS52427.1"
                     /translation="MRGVTRLQTPVLVLNASYEPINVCAARRALVLLLKGVAAAEEEG
                     REFVHSPSRAITVPSVIRLLEYRRIPHQTRALSRKNILIRDRNTCQYCERVLPSAELT
                     LDHVTPRSRGGESNWENLVACCHRCNNRKGNRLPEEAGMRLIRAPRPFSLHTSRHLMR
                     LLGRSDEKWRKYLFF"
     gene            complement(6759..7583)
                     /locus_tag="VFA54_16275"
     CDS             complement(6759..7583)
                     /locus_tag="VFA54_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZS52428.1"
                     /translation="MGPISWRQFALGLPHALFSVLYPDDCRLCSAPLVRFSPAPVCER
                     CLNGVTPLAAAGQCFQCGLPFENQAPLHGADLCGLCRRGATEFDWARGYGAYEANLRG
                     LIHLLKYDGMQPLAKLLGRRLVDLLRQAGPVDLIVPVPLDGSRLRTRGFNQSELLAAE
                     LSRASGVALDASVLRRVRRTETQTGLTHRQRRLNMQGAFSVNPKAAIAGKRIALIDDV
                     ITTGATAGACTRALKNAGAARVIVLAAARARRRIVDVETASARRAEARPGGAICGA"
     gene            complement(7628..7999)
                     /locus_tag="VFA54_16280"
     CDS             complement(7628..7999)
                     /locus_tag="VFA54_16280"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HZS52429.1"
                     /translation="MPVRGRLEKQRLENYRRRLLGKREDLLGLLTRAEQDGRFADEEG
                     TQDLADKASNSYTKEFLFYQSSNDRTLLQMVEEALNRIEEGRFGECVICGGPMDSKRL
                     EAIPWARNCVPCQEKQDRGLL"
     gene            complement(8099..9547)
                     /gene="gatA"
                     /locus_tag="VFA54_16285"
     CDS             complement(8099..9547)
                     /gene="gatA"
                     /locus_tag="VFA54_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZS52430.1"
                     /translation="MENLKTLTVDQVREGLIARRFSAVELAREAIQVAEAENEKYGAF
                     LHFSPERALETAAKVDRQIAAGEPLGALAGVPVAVKDVIVTRGVRTTCASRLLEHYVP
                     PYDATAVDRLEQAGGLIIGKTNCDEFAMGSSTENSAFFPVRNPAAPDRVPGGSSGGSA
                     AAVAAGQAVVSLGSDTGGSIRQPASFCGVVGVLPTYGRVSRYGLVAFASSLDHIGPFA
                     RNVKDAATLLGVIAGRDPADSTSANVPVPDYTSDLDGKVAGFRVGIPREYFGAGLDAE
                     VGARIERGVEVLRRLGCEISDVSLPHTDYAIACYYIICTAEASSNLARYDGVRYTTRS
                     PRSPTLIDMYRNTRGEGFGAEVKRRIILGTYVLSAGYYDAYYLKAQKVRSLVARDFSE
                     AFGRVDAIVTPVSPFPAFKLGEKTSDPLEMYLSDIYTITGSLAGICGISVPCGKTSAG
                     LPVGMQILANHFDEHRMLRLAHAFEQAGGFAG"
     gene            complement(9547..9846)
                     /gene="gatC"
                     /locus_tag="VFA54_16290"
     CDS             complement(9547..9846)
                     /gene="gatC"
                     /locus_tag="VFA54_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZS52431.1"
                     /translation="MKITEKEVRYVADLANLSLTEEEVRKYCADLDEILVYVGKLNEL
                     DVSQVEPMAQVIYEGGENDTLRADEVRPSLDAETALANAPLCGEGQFKVPKVFDR"
     gene            complement(9843..10259)
                     /locus_tag="VFA54_16295"
     CDS             complement(9843..10259)
                     /locus_tag="VFA54_16295"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HZS52432.1"
                     /translation="MRSASELLPQVYRKLTRAGTDEEALLIALWPGAVGEKLASRTRP
                     VRLFGDVLIVETDAQSWRKELARMAAEIVKRLNAAAGTVILRDVEFRVAAAPRPPGRA
                     RFAAPASAEEHDEAAAITDPHLRRLYRLSRRQRDTK"
     gene            10366..10980
                     /locus_tag="VFA54_16300"
     CDS             10366..10980
                     /locus_tag="VFA54_16300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZS52433.1"
                     /translation="MPRRSPTLILASASPRRAEILRRAGIPFRARPAHVDETILPGEK
                     PGACVRRLARLKAEAVSRSGEIVLGADTVVVVGGRILGKPRNARDAARMLRLLSGRAH
                     EVITGICLLRAGDVRSSAAASNGRAASGRVYICDAERTRVWFRRLSRAEIASYVASGE
                     PMGKAGAYAIQGLASKFVTRIEGCYFNVVGLPVSRVWEILQRIA"
     gene            11205..11630
                     /gene="rplM"
                     /locus_tag="VFA54_16305"
     CDS             11205..11630
                     /gene="rplM"
                     /locus_tag="VFA54_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZS52434.1"
                     /translation="MSTHFPSPGEIKSNWYLLDAEGKVLGRLASQAAVLLMGKNKPDY
                     TPFLATGDHVVVINAAKVRLTGKKEQDKVYRRYSGYPGGLKEVGAAKVRATRPTRMVE
                     EAILGMLPKTKLGKQLAGRLRVYAGPQHPHAAQKPRTLS"
     gene            11661..12053
                     /gene="rpsI"
                     /locus_tag="VFA54_16310"
     CDS             11661..12053
                     /gene="rpsI"
                     /locus_tag="VFA54_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZS52435.1"
                     /translation="MMQVQHLGTGRRKTSVARVFLRPGSGEIRVNGRPFNDYFRTESV
                     QAVVRQPLLATETADKFDVMILAHGGGLAGQAGAARLGIARALVNFNGELRQKLKQLG
                     YLTRDARRRERKKYGQKGARKRFQFSKR"
     gene            12222..13133
                     /gene="rpsB"
                     /locus_tag="VFA54_16315"
     CDS             12222..13133
                     /gene="rpsB"
                     /locus_tag="VFA54_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZS52436.1"
                     /translation="MPSITMKELLEAGVHFGHQTKRWNPKMKEYIFGERNGIYIIDLQ
                     KTLKLFKEAAKFVGEAAAQGKTILFVGTKRQAQEAIAEEAQRCGMFYVNQRWLGGLLT
                     NWATVQRSIKRLKDLDRMASDGTYENRPKKEVIRLERERKRLEQNLAGIKEMPGLPDV
                     LFVIDSRKEAIAVKEARKLGIPVVGIVDTNCDPDEVDYVIPGNDDALRAIRLFANKIS
                     ESVIEGRALASEHEFVAEKIITEEAEESGQDLLSMADVPFAEVVNRERDLAEEPTEAV
                     DRHARKGPSSEASEAADVVEAPAVNAE"
     gene            13245..13850
                     /gene="tsf"
                     /locus_tag="VFA54_16320"
     CDS             13245..13850
                     /gene="tsf"
                     /locus_tag="VFA54_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZS52437.1"
                     /translation="MAEVTAQMVKQLREKTGAGFMECKSALAEADGDLQQAELVLRKR
                     GLSFAQKKASRATCEGLIGSYIHAGGKLGVLVEVNCESDFVARTDDFQQLVHDISMQI
                     AATDPRFIRKEDVTPEALEQEKQIQRARALEEGKPEKMLDKIVEGRLNKFYEEVCLME
                     QPFVKDNSMTVGQLVASKIAKLGENITISRFARFKVGESAS"
     gene            13893..>14141
                     /gene="pyrH"
                     /locus_tag="VFA54_16325"
     CDS             13893..>14141
                     /gene="pyrH"
                     /locus_tag="VFA54_16325"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721755.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZS52438.1"
                     /translation="MPKYNRILLKLSGEAMAGEAGFGIEAARVAQIAAEIQETAQAGV
                     QIGIVVGGGNFFRGLQPGPGNLDRVVTDQMGMLATVINA"
BASE COUNT         2826 a         4204 c         4404 g         2707 t
ORIGIN      
        1 cccgctctcc gcaagcctcc ccagagcgac attctcccgg cgccgccggc cccaaagcgt
       61 accattgact gggcgcgcct acgattgatt tcgcagcagc tttaactggc agccagcagg
      121 gctgtgcagc gggcaagcgc cgcttcccaa gaactttgca gttagatccc agaacgagcc
      181 ggaaaggtcc ggtcgcgcca gaccccggat gtcacccgcc caaggcctct tttgcagccg
      241 ccactgtatt gcagtggatc aggaccgtct cgttcacgtc gaaagcgtag ccgccggcga
      301 gcacgatcgc gactccggca ccggtccgca aggcggcgtc gatcaccagc cggtcacggc
      361 gcttcagtcc ttccagactc agagaaaggc cgccgagctg gtcgcggcag tagggatcgg
      421 cgccggcaat atagaacacc agttggggcc ggaagcgggc ggccgcggga atgtactttg
      481 ccgaaagcgc cgccaaatac tccgcgtccc cggttcggtc ctcaagatga atgtcaatgt
      541 cactcggagg cttctccgct ggatagttat tgtattggtg aatcgagatt gtaaataccg
      601 ccgggtcgcc ggcgaagatg gcggccgtcc cgttgccgtg atgaacatcg caatcgatga
      661 cgagggcgcg ttcgatcacc tgatccttca gaagccgccg ggtcgccacc gccacatcgt
      721 ggatggcgca gaatccctct ccgtggccgg gaaaggcgtg atgaaagccg ccgctgagat
      781 tgacggcgat ccggtcgatg agcgcccgcc gcgctgcgag aatcgatccg ccggtcgcca
      841 accacacagc ttccaccagg ggccgggaat aggggatctc catgcgggcg atttcttgat
      901 agctgagagt tccggttttc agccgctgga catatcccgg ttcatgaacc agcagaatgt
      961 cttcgtcgga cgccggagcc ggctcgagga agtcttccgg cgcggcgagt ccctcccgca
     1021 ggagccggtc gcgaaccagc cggaatttct gtgacggaaa aacgtggcgg ccgagattca
     1081 aatcataacg ctcggaatag accagcttga agggcagcac cattaccggc ctgtgtccga
     1141 ctgcaaagct ctggccgcgg cggtgtagaa ggacatagga gcatcttact accagtagcc
     1201 tggaagctag gggaagccaa cccaagccga ggcaggcggc cttcgggagc ttccgcatcg
     1261 atgatgcatt ttaggcgcct tccatggccg taaatagatt gttcagcgat tcggccagag
     1321 tgggatgagc gaaaattccg tcgcgcagag ccgtatacgg aagcttgccc atcatggcca
     1381 cctcgaggac cgccataatc tcgccgcctt ccgttcccag aaccgccgct cccagaattt
     1441 gcgcagagcg tgcgtctaca acggctttca tgaaaccgcg gctctcgccc gtttccagcg
     1501 cccgcgcaac ggagctcata gggagcttgg caatgcgaat ttcgcgccct tgcgcccgcg
     1561 cttccacctc actcagtccg acgcgtccca attgcggatc ggtgaatacg gtatacggca
     1621 cgagccggcc atccgtcgtg gcgttgccat tctcgatcag attctcgcgg atcacgcggt
     1681 aatcgtcata ggaaatatga gtaaacgcag ggcccccttt gacgtcgccg agcgcccaca
     1741 cgctttccgc cgtcgtttcc agcctgctat tggtctggat aaaccccttt ttgtccgtag
     1801 cgattccagc ggcgggcagg tcgagggctt ccgtgttggg ccgtctgccg gtcgcgacga
     1861 gcaaatgcga gccggtgaac gtgcgctcgc cgacggtagt gcgaacgcgg agctgaatgc
     1921 tgcttccggc gagcgaggcg cgaaaggtct gggcggccag gagcacttct ataccatcct
     1981 cgcggagaat tttggcgacc tcatccgcga cgtcggggtc ttcgcgcgcc agcagccggt
     2041 cgcccgattg aatcatggtc acctggctgc caaaccgccg gaacatttgg ccgaattcca
     2101 ccccgatgta tccgcctccc aggaccagca agcgctccgg aagcgtttcg agctccatga
     2161 tcgagacatt gtcgagcgcc ggaacctcgg tgatcccttc cacggccggc agcgaagcgc
     2221 gggtaccggt gttgatgaag attttggcgg cggccatgcg ttggatgcct ccgctattca
     2281 gccgcacctc tatccgcttt ggccctgtga accgcgcttc gccgaaaatg agatccaacc
     2341 ctggcgtttg ctccagccgc tgctggctac tcttccgaaa gctgtccacg atcccgcgtt
     2401 tgcggtcgcg gactcttctc atgtcgactg ccgccgaggc cgtgcggacg ccgtaatcgg
     2461 cggcgcggcg ggccaaatcg gcgacccggg cgctggcgat catggttttt gtaggcgtgc
     2521 agccctcgtt gacgcaagta ccgccaacgt gccggcgctc gatcagggcc gttttcgatc
     2581 ccgctcgggc cagcgccatg gcgagcgggg ttccggcctg gccggatccg attacgagag
     2641 cgtcgtagta aatctcgctc atttcttctg acctcccgct ggcgtcacca tcgtataaaa
     2701 tgcgttgcgg cccgagcgaa gatcctttcc gtacgaaatt accaggcgga ccgcgcccaa
     2761 gacgccgatt ttgcactgcg cgcccggatc caccagccag cgctccgagc cgaagccctg
     2821 ggattcgccg taaattcgcc caccatcgag caatgggccc gcctttacgg tgacaaggct
     2881 gttgacgtaa agcgctttgt caatttccca gttcagcaac aggtagtcgc gtcccagcgg
     2941 ggcgctgcct tttttcccgt cctgcgtgcc gatatggccg tgcagccaga ggtcgttgtc
     3001 gcgctcgatc cccagtatga acagctcgtc aaaggggacg gggccgaacg ttttgcccgc
     3061 ccggtagtgc gctgtcattt cataatcttc ccctcgcggc cgtggatacc aatgaaaatc
     3121 gaccgaacct tgcagcttgg aaaaggcgtg gaaggatggc gcgaagagcc gtccaaattc
     3181 ccaggagccc gtggaatcca gcgtcaagcg gtgctccgaa agccggagga ggttgtagtc
     3241 cacgccagca cggtacccga gcgaaaggcc gcccgtaaat acgggagaca gcagggacgg
     3301 agggagcacg gcgctccgaa attcccggtc gatcaggttg acgcccgcct gccaggcgaa
     3361 tttcccattg acggcggacc ggatccccgc tcctgcctcg attctttgca gattcaggcc
     3421 ggagagcggc gtagcgctca ggtggaacgt tcgggagaga tcccagtttt cctttctgcc
     3481 atctacatac gcctgatagc gccaggcggg ctttccgtgc aggggcgccg agaacattgc
     3541 caggacgcgc ctcttttgag cgtcccagcg aaacagcgac gtgatgttca ccgcggagcg
     3601 ttgcagattg taaaactcgg gggtgatcgt ctggtacggc gttcctcgca gcagtgacaa
     3661 aagcccgccg agggcgccgt cgccccagcc gtttcgttcc acagggcgaa acagcaagtc
     3721 aaagttcgag ccgtccgcag gcaacagctc gaagcggaat cgcgggaaga tgctcaactc
     3781 gcggagacgg gcctcggtgg tccagaaatc ctcgccgcgc agcaggctgg cgggagaaaa
     3841 agcgaaagcg cggtcgagaa gcacggggtt gacttgaggc tgaggctcga ttcgcaagtt
     3901 ctcgatgtgc ggtttttgga tgcggttcca atatttcaac gcggcgggaa gattttcgtc
     3961 gagtacgtac agcgttgcca ggaagtcgtt ggcataagcg tcttcagggt ccagccggag
     4021 agctgtctga atttcctccc tggctctcga cagatccttg cgtttgaagg cgacgccggc
     4081 gagctccaga gggaaccgct tgtcacgcgg cgcctttctc cggcctgttt tgaaggcgtc
     4141 ttccgcatcg gcccagcgct ccagccgcgc cagagccata ccccggtagt aatccagatc
     4201 ggcggaatgc tccgaccccg ggggtacggc atctacaact tcctgccagc gctgctgcgc
     4261 gtagagtaaa gcgacgcggg aggcgggatc ggccgcgaat ccggaacgat caccagcgga
     4321 aagaagggcc agcagggcga ggatccgcgc cgcagacttc actgcgtttg ctggggaatc
     4381 gccaaaagcg tccaattctg tgcgccgccc cactgtttct cgaaagcgga ccgaggcatc
     4441 ttcagcagct tgcgctgcgc cgggtcgttg acgaggatga gattctggtc ccaatcgaaa
     4501 cccaccacga caacatagtg tagtgggttt ccggcgccgt tttctttcag gcaaacgatc
     4561 aggggctggc ctttggagag ccgcggttta aagtcttccc acgagccccg gaaaacaaag
     4621 gtacgaaagc cattggcttg aaaatagcgc tccatgtccg aggctcggat gccggcttgg
     4681 tgttgcgtat acagcttttt ctgaatacac ccggcgtcgg ttgcttccgg aggcacttgc
     4741 gcgccgttgt gcgcccaata ctgagcgacc atggcgatca ccgcggagcc gcacccgttt
     4801 cgctcctgct tgacataagg gacgtcgagc cagatgccgg acgacgcaaa tccgagggcc
     4861 agggctgcga cagcggctat cgccaggcgc accataatgc tcaagccttc actgccacga
     4921 tgataatgat cagggcaatg agcagcaaca aaagcagagt cagcgagcct attcccagaa
     4981 agccggcttc aaagtcgcgc tggatctgct cggcgcgcat ggaaatactg gcaagttcct
     5041 catctgtcag ttgagggatc gcctgctgga cttttgtgga atcgatgccg ccggctttca
     5101 ttgcctcctg aacgggcggc agggagaaaa agcgctccag ttttgcgatg tcgagctgtc
     5161 tctcgcgcat cttggcctgc aacgcttgtt gcaaccctgc tgggccgaca acatgatctt
     5221 cagtctgtgc ggcggggaag caaagcggcg cgagcatcaa gaacagcagt acgcggtgga
     5281 tgtgtgtaag gtgaccagcc acaaagcctc ctgttccggg gggcagtccc gcacggggga
     5341 gctcggcggg taagccgacc tctagatacg cgactgcaaa aatcctgcca aagaggatca
     5401 cgcggccatc gcgtggcggg atcccgccca gggcgcgccg ccggctattt tgcctccaca
     5461 atcaccagaa tgatcactgc ggtggcgagc gcaatgatga tatatgtaat ttcctggttg
     5521 gtgagggcgc ctgcttcgaa gtcccgctgg gccttctcgg ctcgggaggc caatcgtgcg
     5581 agttcgtcgt cgctgagctg cggtatcgct ttttgaatgg cgctgtaagc gagcccgctg
     5641 ctttcgagcg ctttctttac tcgtggaatc gaaaaaaaat ggtccatctt ggccagattt
     5701 ttttgtctgg cgtccgacac agcttccatc tgcttttgca gatccgcggg gctaaccacg
     5761 tgatcctcgg cgtatacatc cctcggaagg actaacagtg cggtcaggac aaaaacaatc
     5821 aatggagtag tcttcgcgta gagccgcatt cggcctcctt catgagtatg atcgccactt
     5881 ggcagagagt tttctcccgg gcggcatatc ttttctttgg gtgctggcga actcctgcat
     5941 caccggcggg cgcatctggg ggccggggca gcagcagagc cgcctgtttt ggcccgctga
     6001 ctgccgtttt ttcggcgctc agccacaaac cgccatcctg ggccgcagac tgccattgtg
     6061 taatggcacg aactttgcca aatcctatat tctacgtaag ctccttattc tctgtgggcg
     6121 ccggctcaca ttgcctttcg aacggcgtgt aacgtaaggt gtcgtacgaa tttcttacac
     6181 ggtgtgccag gcagacctgc ggtgaacgtt ccttccgggg tccgctctct tttacagctt
     6241 gattcagaag aaaaggtact tgcgccactt ttcgtcgctg cgaccaagca ggcgcatcag
     6301 gtggcggctg gtatgaaggc tgaacggccg cggagcccga atcagccgca tccctgcttc
     6361 ctcgggaagc cggttgccct tccggttgtt gcaccgatgg cagcaggcga ccagattttc
     6421 ccagttcgat tcgccgccgc gcgaacgcgg cgtgacgtgg tcgagggtca attcggccga
     6481 aggcagcacg cgctcgcaat actggcacgt attgcggtcg cggatgagga tattttttcg
     6541 ggaaagagca cgggtttgat ggggaatgcg ccggtactcc agcaaccgga tcaccgaagg
     6601 caccgttatg gcgcgagaag gggaatggac aaactcacgg ccttcctctt cagcggcagc
     6661 cactcccttg agcaggagca ccaaggcccg gcgcgcggcg cacacgttga tcggctcata
     6721 gctggcattt agaaccagaa ccggtgtttg gagccgcgtc acgccccgca tatagctccc
     6781 cctggacgcg cctcggcgcg gcgtgcggaa gccgtctcca catcaacaat tcggcgccgg
     6841 gcccgggcgg ccgcaaggac gatgacgcgg gccgcccccg cattcttcaa agcgcgcgtg
     6901 cacgctcccg cggtagcgcc cgtcgtaatt acgtcgtcaa tcaaggctat ccgttttcct
     6961 gcaatggctg ctttcgggtt cacagagaag gcgccctgca tattgaggcg gcgctgccgg
     7021 tgcgtaagcc cggtctgcgt ttccgtgcgc cgcacgcgcc gcaatacgga cgcatcgagg
     7081 gctactccgg aggcccggct caactcagcg gccagcaatt cggattgatt gaagccacgc
     7141 gtccgcagcc ggctcccatc cagcggcacc ggaacgatca ggtctaccgg tccggcttgg
     7201 cggagtaaat cgactagccg ccgccccagc agttttgcca gcggctgcat gccgtcgtat
     7261 ttgagcagat gaataagacc gcgtaaattt gcctcgtagg cgccgtaacc gcgagcccaa
     7321 tcgaactcgg tcgcgccgcg gcggcaaagt ccgcataagt ccgcgccgtg caaaggcgct
     7381 tgattttcaa atggcaagcc gcattggaaa cactgccccg cggcggccag cggggtcacg
     7441 ccgttcaggc agcgctcgca tacgggcgct ggcgagaacc ggaccagcgg ggccgagcac
     7501 aagcggcagt cgtcagggta taaaacggaa aaaagggcgt gcggcagccc cagcgcgaac
     7561 tgacgccagg aaattggacc cattgcgggc tggctacaac tactcgagaa gactcacctc
     7621 cgaggcttca gagcagacct ctatcctgct tttcctggca cggcacgcag ttccgcgccc
     7681 agggaatggc ctccaaacgc ttgctgtcca ttgggcctcc gcaaatcaca cactcgccga
     7741 agcggccttc ctcgatgcga ttcaacgctt cttccaccat ctgtaagagg gtgcgatcat
     7801 tgctgctctg gtagaacagg aactctttgg tgtaggagtt ggaagccttg tcagccaggt
     7861 cttgggtgcc ttcttcatcg gcgaaacggc catcctgctc cgcgcgcgtc agcaggccga
     7921 gcagatcctc gcgtttgccc agcagacgtc tccggtagtt ctccaggcgc tgcttttcga
     7981 gccgtccgcg cacaggcata caaaggcacc ttcaagaaat gattgtagga attcacgatt
     8041 gtccttgtca atcgtggcgc ggacggaggg tgcagccgct gccgcacggg cgttccccct
     8101 agccagcgaa tccacccgcc tgctcgaaag cgtgagccag ccgcagcatc cggtgctcat
     8161 cgaaatggtt agcgagaatc tgcatgccga ccggcagccc ggcggaagtc ttgccgcaag
     8221 gcacgctgat cccgcaaatg cctgccaggc tgccggtaat ggtgtaaata tccgacaagt
     8281 acatttcgag cgggtcggac gtcttttccc ccagcttgaa cgctggaaag ggcgaaaccg
     8341 gggttacaat ggcgtctacg cgcccaaagg cttccgagaa atcgcgcgcg acgagcgacc
     8401 ggactttttg ggctttcagg taataggcgt cgtagtagcc tgcgctgagc acgtacgtgc
     8461 cgagaatgat ccgccgtttc acttctgcgc cgaatccctc gccacgggta ttgcggtaca
     8521 tgtcaataag ggtcggcgag cgcgggctgc gggttgtata ccggaccccg tcatagcggg
     8581 ccagattgga gctcgcctcc gcggtacaga taatgtaata acacgcaata gcatagtcgg
     8641 tatgaggcaa gctgacgtcg ctgatctcgc aacccagtcg gcgcagaact tctacccccc
     8701 gctcaattct ggcgcccacc tccgcatcga gtccggcgcc gaaatactct cgtgggatcc
     8761 ccacgcgaaa gccggcgact ttgccatcca ggtccgatgt atagtccggt acgggtacat
     8821 tggcggaggt ggaatcggcg ggatcgcgcc cggcgatgac gcctagcagc gtcgcagcgt
     8881 ctttgacgtt tctggcgaac ggaccgatgt gatccagcga gctggcaaaa gccaccaggc
     8941 cgtaacggga aacgcggccg taggtgggca gcactcccac aacaccgcag aaagaagccg
     9001 gctggcgaat cgagccgccg gtgtcggacc cgaggctcac gaccgcctgg cctgccgcca
     9061 ccgccgctgc tgagccgccg cttgatcctc ccggcacgcg atcgggggca gccgggttcc
     9121 gaactggaaa aaacgcggag ttctccgttg acgaccccat ggcgaactca tcgcagttgg
     9181 tcttgccgat gatcaagcct cctgcctgct cgagcctgtc cacggcggtt gcatcgtagg
     9241 gaggcacgta atgctcgagc aggcgcgatg cgcaggtggt gcgtacgccg cgggtgacga
     9301 tcacgtcctt gacggccacc ggcactccgg cgagggctcc caaaggctcg ccggcggcaa
     9361 tctgccggtc tactttcgct gccgtctcga gcgcccgctc cggcgaaaag tgcagaaagg
     9421 ctccgtactt ttcgttctcg gcttcggcaa cctgtatggc ctcgcgggcg agctcgacgg
     9481 cgctgaagcg ccgcgcaatc agcccctcac ggacctggtc caccgtcaat gtcttcagat
     9541 tttccatcag cgatcgaata ccttcggaac cttaaattgt ccttcgccgc agagcggcgc
     9601 gttggcgagc gccgtctcgg cgtcgaggga cggccgcacc tcgtccgccc gcagcgtgtc
     9661 attctccccg ccttcataga ttacttgcgc catcggctcg acctgtgaga cgtcaagctc
     9721 gttgagtttg ccgacgtaaa caagaatctc gtccagatcc gcgcagtatt tgcggacctc
     9781 ctcttcggtc aaggataagt tggccaaatc ggccacgtag cggacttcct tttcggtgat
     9841 cttcattttg tgtcccgctg ccggcgggac aagcgataga gccgccgcaa gtgcggatcg
     9901 gtgatcgcag cggcttcgtc gtgctcttca gcgctcgcgg gcgcggcgaa acgggcgcgt
     9961 cccggaggcc ttggcgcagc agctacgcga aactccacgt cccggaggat aaccgttccg
    10021 gctgcggcat taagccgctt cacgatctcg gcggccattc gggccagctc tttgcgccag
    10081 ctctgggcat cggtttcgac gatcaagaca tcaccgaaca gccgcacagg ccgggtgcgc
    10141 gaagcaagct tttctcccac cgcgccgggc cacagggcga tcagcagcgc ctcttcatca
    10201 gttcccgccc gcgtcagctt ccgataaact tgaggcagca gctctgatgc agaacgcata
    10261 gcgatttccg aaacgagtga gttaagatcg cagcaagctt cagtgctggc gagcgccgta
    10321 cgtggctgcg tacgcggctg tcggctcgaa aacgccaatt ttagcatgcc caggagaagc
    10381 cctaccctga ttcttgcttc ggcttcgccg cgccgggctg aaattctgcg ccgggcaggc
    10441 attccgtttc gagcgcgccc ggcacatgtt gacgagacga ttttgccggg agagaaaccg
    10501 ggagcctgtg tgcggcgcct ggcgcgcttg aaggccgagg ccgtctcccg ttcgggcgag
    10561 attgtgctgg gggcggacac cgtggtagtg gttggaggcc ggattctagg gaagccgcgg
    10621 aatgcgcgcg atgcggcccg gatgctgcgt ctgctttccg ggcgcgcgca cgaggtaatt
    10681 acaggaattt gcctcctccg cgccggtgat gtccgctcca gcgccgccgc atctaacggc
    10741 cgcgctgcgt ccggccgcgt gtacatttgt gatgccgaac gcacccgcgt ctggtttcgc
    10801 cgcctgagca gagccgagat tgccagctac gtcgcttcgg gagagccgat gggcaaggct
    10861 ggcgcctacg ccatccaggg actggcttct aaatttgtaa cgcgcatcga gggctgctac
    10921 ttcaacgtgg taggtctgcc ggtctcccgg gtttgggaga tattacagcg gatcgcataa
    10981 tttcttgcaa ctttgtttgg gcggccgggc atcggctcca tccaacggag aggccgatcg
    11041 cgaccggcgg gggctgccga tttcgcgcgt atgcggtatg ctagaagagg cgcagcggac
    11101 acggccgcaa atccagcccc aactcgtgcc tcaatgtttg acttggaggc tcggctcggg
    11161 taagctaaat atttttggat aatagggatt ggagttcttt agggatgtca acgcattttc
    11221 ctagcccggg ggagatcaag agcaactggt acctgctcga cgcggaaggg aaagtactgg
    11281 gacgcctcgc tagccaggca gcggtgcttt tgatgggcaa gaacaagccg gattataccc
    11341 cgtttctcgc cacgggggat catgtggtgg tcatcaacgc agccaaagtg cggttgaccg
    11401 gaaaaaaaga gcaggacaag gtctatcgta ggtattccgg ctacccgggc ggattgaagg
    11461 aagttggcgc ggccaaagtg cgggctacgc gcccaaccag aatggtggag gaagcgatat
    11521 tgggaatgct gcccaagacg aaactgggca agcagttggc agggcgtctg cgcgtctatg
    11581 ccgggccgca gcacccgcat gccgcccaga agccgcgcac gctttcgtag ccgaaggaag
    11641 gaagcggcat tttgttttcg attatgcaag ttcagcatct gggcacaggc aggcggaaga
    11701 catcggtagc cagagttttt cttcggccgg gcagcgggga aatccgcgta aacggccggc
    11761 cttttaacga ctattttcgg acagagtccg tacaggcggt ggttcgccag cccctgctgg
    11821 cgaccgaaac cgcggacaaa tttgacgtca tgatcctggc gcatggcggg gggctggccg
    11881 ggcaggccgg cgcggcgcgc ttgggaattg cgcgcgctct ggtcaatttc aatggggaac
    11941 tgcgccagaa actgaagcag ctagggtacc tgaccaggga tgctcggcgg cgcgagcgca
    12001 agaagtatgg gcagaaaggc gcccgaaagc ggttccagtt ctccaagcgc taaaaagcag
    12061 cgtcagggtt ttgggtgttt cacacgcggc ccccttccgg gatgggcggg gcggcgagca
    12121 atcagtaggg atcatcccgc cgtcttgcgt cgagggctca aattccgctc ggacgagccg
    12181 ctcgttgcag gacgcgaagc ccgcagggca gaggaggatt tttgccgtcg attacgatga
    12241 aggaattgct cgaagctggc gttcacttcg ggcaccagac caagcgctgg aatccgaaga
    12301 tgaaggagta tatcttcggc gaacgcaatg gaatttacat catcgatctg caaaagacgc
    12361 tgaagctttt caaggaagcc gccaaattcg tgggcgaggc cgcggcgcag ggcaagacga
    12421 ttctctttgt aggaacaaag cggcaggcgc aggaggctat cgccgaggaa gcccagcggt
    12481 gcgggatgtt ttacgtcaat cagcggtggc tcggggggct gctgacaaat tgggctactg
    12541 tgcagcggtc gatcaaacgg cttaaggacc tggaccggat ggcttccgac ggcacttacg
    12601 agaaccggcc gaaaaaagaa gtgatccggc tcgagcggga acgcaaacgt ttggagcaaa
    12661 accttgccgg gattaaggaa atgcccggct tgcccgacgt gctgttcgtc attgactcgc
    12721 gcaaagaggc gattgccgtc aaggaagccc ggaaactggg tatcccggtg gtgggaatcg
    12781 tggacaccaa ttgcgatccg gacgaggtcg attacgtgat ccccggcaat gacgacgcgt
    12841 tgcgggcgat ccggctgttc gccaacaaga tttctgaatc ggtgattgaa ggcagagcgc
    12901 tggcttccga gcacgagttc gtggccgaaa agatcatcac ggaagaggcg gaagaaagcg
    12961 gccaggatct gctcagcatg gccgatgtgc cttttgccga agtcgtgaac cgcgagcggg
    13021 atctggcgga agagccgact gaagctgttg accgccatgc gcggaagggc ccctcgtcag
    13081 aggcttccga agcggcggat gtagtggaag ctccggcggt gaacgcggag tgatccgtcc
    13141 ggattgacga gtgggtgggg ccagaacccc gtggggcggc gcgtccgccg ccgtcaatct
    13201 tcaaaatcaa tttaaatcgt caacttctct tgataggtgg tctcatggcg gaggttaccg
    13261 cacaaatggt aaagcagctt cgggaaaaga ccggggctgg gttcatggag tgcaagagcg
    13321 cgctggcgga ggcggacgga gacctccagc aggccgaact ggtgctgcgc aagcggggac
    13381 tctccttcgc gcaaaaaaag gcctcgcgcg ccacgtgtga aggccttatc ggttcatata
    13441 tccatgcagg cgggaagctc ggcgtgcttg tggaagtgaa ctgcgagagc gacttcgtgg
    13501 cgcgcaccga cgatttccag cagcttgtgc acgacatctc gatgcagatc gcggcgaccg
    13561 atccgcgttt catccggaag gaggacgtca cgccggaagc tctcgagcaa gagaaacaga
    13621 ttcagcgcgc ccgggcgctc gaagagggca agcccgagaa gatgctggac aagatcgtgg
    13681 aaggccggct gaacaagttc tacgaagagg tgtgcctaat ggagcagcct ttcgttaagg
    13741 ataacagcat gacggtgggg caactggtcg cttccaagat cgccaagctg ggtgaaaaca
    13801 tcaccatcag ccggtttgcc cgattcaaag tgggcgagtc ggcgagctag ccgggctgcc
    13861 ggggcaagag gcgcagcccg cagccttcct ttatgccaaa atacaaccgg atcctgctaa
    13921 agctcagcgg cgaagcgatg gccggagaag cgggctttgg catagaggct gcgcgggtgg
    13981 cgcagatcgc cgcggaaatt caggagaccg cgcaggccgg agttcagatt gggatcgttg
    14041 tcggaggagg caattttttt cgcggcctcc aacccggacc cggcaacctg gaccgcgtcg
    14101 tcaccgacca gatggggatg ttggcgacgg ttatcaacgc g
//
LOCUS       DASREK010000300        10512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_482648, whole genome shotgun sequence.
ACCESSION   DASREK010000300 DASREK010000000
VERSION     DASREK010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482648"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFA54_16330"
     CDS             complement(<1..552)
                     /locus_tag="VFA54_16330"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS52439.1"
                     /translation="MLKRVLGALFALVLLSADAVAQEAGQIVGTVRDQSGAVIPNATV
                     TATEAGTGFVHTAVSGNDGNYVLPNLRPTTYVISAEAQGFRAFRQTDVRLQANQSLTI
                     NIAMEVGAVTETVNVAGQVVQVDTSTSTLAEVVDESRIVELPLNGRDAAKLSTLVAGT
                     VMISTSTETGKGIPGNFYLSANGS"
     gene            complement(1029..1958)
                     /locus_tag="VFA54_16335"
     CDS             complement(1029..1958)
                     /locus_tag="VFA54_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS52440.1"
                     /translation="MKLTKSTGSCGRRSLQVLAVALLSAAALPVYGQRNRSNQVQPAG
                     PTNPFSGNQQAVAEGEEIYNRTCTACHGKDGTAGERAPALGAPARRYLRRTDADIFDA
                     IEKGIPGTAMPPTGLSETDAWKVAAYIHGLRGAAIDTPVKGDVAHGEQIFWGKGNCGN
                     CHMLHGKGGLMGPDLSNIAGLRKLNSIVDALTKAQHKVPTDGGTHDAALQPLSTYQPV
                     RITTRDGKIITGVLRNEDSFSLQVLGSDNELHLFERADLQQVYYVPKSIMPTDYDKRL
                     TPAEFQDLLAFLSRQAIVAPETPAGRGGRPAGE"
     gene            complement(2042..3619)
                     /locus_tag="VFA54_16340"
     CDS             complement(2042..3619)
                     /locus_tag="VFA54_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZS52441.1"
                     /translation="MKTKLLLVCLLWAAACTAAAQVTSEDLLKPNPNNWLTYSGSYNA
                     QRHTLLNQINTSNVANLQAKWIYHMSGAVDFEAVPIVVNGVMYISQFNRVDALDGRTG
                     RLIWQYQRQPPTRGWQRGVAVYGHRVYVTTADSCVIALDARTGALVWETKAPGGVRLQ
                     GGAPLIVKGKVYVGGSGRTGGFVQAYDAETGKHLWTWNVIPKPGEPGSETWEGDSWKL
                     GGGPTWLTGSYDPDLNLLYWGTGQPGVDFVGEIRKGDNLYTDCMVALDPDTGKLKWYF
                     QFTPHDVHDWDAVEIPVLVDAEFKGQMRKLLIQANRNGYYYILDRTNGKFLFGTPFVN
                     AVTWSSGLTEDGRPVVVPGTDPTVQGNKVCPSTAGATNWPSPAYNPDTHYFYLIAQEG
                     CGINYKASDNFRPGGAGFNSGTGYMESPDQQERWQLFVRALDLTTGKKVWDYEEIGSH
                     HYGPGLLSTAGGVLFAGEQQGQFTALDARTGKVLWHFNTGDLITASPMAYSIDGKEYV
                     AIGSGTNVIAFGLPDGK"
     gene            complement(4111..4968)
                     /locus_tag="VFA54_16345"
     CDS             complement(4111..4968)
                     /locus_tag="VFA54_16345"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZS52442.1"
                     /translation="MGVSNASRSIGLPDQAALIARAKQGDREAFSELYEPLEKPLGAF
                     LYRLLAVREDAEDLMQETALKALESIADFPGDSSFRTWMFRLAAEAGLAHLQGKRQWD
                     PDAVIRAGQIAGEDPGQRRKLQKLHRSGTHSTYEIREHIDFCFTSMGRTLPPEENAAL
                     LLIDVHGFTLEEAAETLGISGKLVRFRLEQARQAMIDRYESRCGLINKNGSCSQCAAF
                     DTLLHGDRRHTEQALFQIEIDPQPNAQARAETFPKRLGIVRAADPLHGEGRKIHDLLM
                     TFTRELNGY"
     gene            complement(5128..6651)
                     /locus_tag="VFA54_16350"
     CDS             complement(5128..6651)
                     /locus_tag="VFA54_16350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZS52443.1"
                     /translation="MALFKTRSAAVYGIDAHLIEVEVDMYAGSAREFITVGLPDVAVR
                     ESRERVKSSLINCGFGYPNKSVTINLAPANVRKEGAGFDLPMAVGILGAMGTLAPQSL
                     QDCLLVGELSLDGGLRPIRGALSIAVCAQQMGIRHLVLPKENGPEAAVVEGISVHGLG
                     SLGEVVERIGKWDRFPTLTRAAGARLRANGSGLDFRDVKGQQATKRALEVAAAGAHNL
                     LMIGPPGSGKTMLAKRLAGILPELTFQEALETSKVHSVAGVLNPEEGILQERPFRAPH
                     HNVSDAGLIGGGMGVARPGEVSLAHNGVLFLDELPEFARNTLEVLRQPLEEGSVTIAR
                     SNMTLTFPSRFMLVAAMNPCPCGYYGDPTRECRCTPPIIQRYLGKISGPLLDRIDIHI
                     EVPAVKYKEMRADAATEGSAEIRGRVERARELQKARGYYNAQLPTRLLRKYCALDEAG
                     ERTLELAVQRMGLSARAHDRVLKLARTIADLDRSEMISAKHLAEAVQYRSLDRSYWN"
     gene            7220..9322
                     /locus_tag="VFA54_16355"
     CDS             7220..9322
                     /locus_tag="VFA54_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52444.1"
                     /translation="MIRKGVARERIGRTVLGRKTIAPVLAVLFSLSVLPAADLPVRKV
                     TLYKDGIGFFERAGRVPAGETARLEFTADQMNDILKSLTIEERGGGRVIGVRYDSSEP
                     LARKLQEFPFAISSGQAVPAILDQMKGARVELHFGSAGGAERVQGIIVSARSAAASER
                     QAEKQELILLLDSGDLRAVDLLAASGIRFMDPKLQAQFQEYLIALGQSRNRDKRGLLI
                     ESSAAGPRDLIADYIIPAPAWKTSYRLIFDSNAEPMLEGWAIVDNLTGEDWTNISLSL
                     VSGLPVSFVTRLYEPRYVARPEAELPGDRAQRPILHAAAMEELQSAANRAAAAPVLRG
                     APAVQAGVGGGVVGGWPDREFRKERDAKPLERAEFASSLAQTAGGRELGDLFEYRIGH
                     PVTVRKEESAMLPFLQQRIKAERLLVYSESYGSQHPLAAVQITNSTGTTLDGGAITVI
                     DAGAYAGESLMETLKAGDKRLVSYAVDLGARVTTAFDSQSQLLREFHFRRGVLTTRTA
                     IQETKTFTVRNVDSRSKTLLIETPVRPEFKLLDIQPLETTPNAHRFALKLGPNTTQRF
                     PVREERVIENSIAISSLTPDVLMTYVQNRNLEDASRKKLEPLLTLKQQLASAQAEAQR
                     VEKEIQDLFQDQQRLRQNIDSLNRVNGQQQRVQEYAQRLASQESQLAGLRDRQSELQR
                     RRASLEQQISKLVDALEF"
     gene            complement(9359..>10512)
                     /locus_tag="VFA54_16360"
     CDS             complement(9359..>10512)
                     /locus_tag="VFA54_16360"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZS52445.1"
                     /translation="PDWAQEAGYGQRLIGPGFILRGNRETVRGNRIFGNGYHGLILES
                     DLLGSEGPSAPSENTIEENMFAWNGGNGVSVDGKNGVSPSRSNIIRFNLFQQNNQARA
                     GSGMDAELRLAGNVGGALIYNNTFYADRSNGLLLYAASVSAGTAQGADAAPSGIRILN
                     NITVEASAAPFLYPLRAVDPGPGLLADYNDWYRAVGGPLASWSGADLTSLEALQEKTG
                     LESHGLTTDPKFVSAENGWFWLRAISPVIGRGLTDVPSSKIPPAPMAASPDLGAFPYR
                     PLLNTSSTELRFVTFFGARDPAPQTLKILSAGPAPIRWSARTEAPAWLGISQSAGSTP
                     AEISVLVRPAGLGIGAYHAAIQLSPALAGEPASVVQVTLSILPVPPRRR"
BASE COUNT         2059 a         3228 c         3085 g         2140 t
ORIGIN      
        1 ggagccgttg gcagagagat agaaattgcc agggatgccc tttccggtct cggtgctggt
       61 ggaaatcatg acggtgccgg ccaccagcgt gctgagcttg gccgcgtcgc ggccgttaag
      121 cggcagttcg acgatgcgcg attcatccac tacttccgcc aaagtggatg tgctggtgtc
      181 aacctgcacg acctggccgg ccacgttgac cgtctcggtc accgcgccga cctccatggc
      241 aatgttaatc gtcaggctct ggttggcctg caaccgcaca tcggtctggc gaaatgcccg
      301 gaacccctgg gcttcagccg aaattacgta ggttgtgggc cgcaggttgg gcaagacata
      361 attgccgtca ttaccgctga cggccgtatg cacaaagcct gttcccgctt cggtggccgt
      421 taccgtcgcg tttggaatca cggccccgct ctgatcgcga accgtcccca caatctggcc
      481 cgcctcttgt gccactgcgt ctgcgctcag caagacaagc gcaaataatg cccctaatac
      541 acgtttcagc ataaagtaag ccctccaaac tgctcacgaa acaagaaagc caaacctatc
      601 aactgtgtcc ccctgaagaa gaagacacgc acataaactc caccaacgca agccttgggc
      661 gaaatggtat caccggccgt ttcagccgtc aagggaaaac ggtgttttta taggaatttt
      721 atgggcagag gtccttccgt cggaccaagc caactcggtt tatcccggtc accaataaaa
      781 taacttaata tttttcaata tattaacaga agtctaggtg cagctcctct caaggcggaa
      841 ggagcctgta gcgcgtggtt tttgcttatt ttcttatatg aaatgggtct gccggacgcg
      901 tgagttgctg attgttgtcg ctgccactgg acgtaggacc ctggcagtcg taaagtacca
      961 ccagcgagtg aaaatcaggc cggcacgtct gagcaaggaa atgcttgagc aaggatcggc
     1021 agggccgcct actcacccgc tgggcgcccg cctcttccgg ccggcgtctc gggcgccact
     1081 atcgcctgcc ggctcaagaa ggcaagcaag tcctggaatt ccgccggggt cagccgctta
     1141 tcataatcgg taggcatgat gcttttcggg acgtagtata cctgctgcaa atcggcgcgc
     1201 tcaaacagat gcagttcgtt atccgacccg agaacttgaa gagaaaaact atcctcgttc
     1261 ctcaaaaccc cagtgatgat cttgccatcg cgagtcgtaa tccgcactgg ctgatacgtg
     1321 gataaaggct gaagggcggc atcgtgggtc ccgccgtcgg tgggcacttt gtgctgcgcc
     1381 ttggtcagcg cgtccacgat ggagttcagc ttccgcaagc ctgcgatgtt cgacaagtcg
     1441 ggccccatca gcccgccttt tccgtgcagc atatggcaat tgccgcaatt ccccttgccc
     1501 cagaagatct gctctccatg ggctacatcg cctttgactg gcgtgtcaat ggcggcgccg
     1561 cgcaagccgt ggatgtaagc cgccactttc caggcatcgg tttctgagag gccggtaggg
     1621 ggcattgccg tacccggaat gcccttttcg atcgcgtcga aaatgtcggc gtccgttcgc
     1681 cgcaagtatc gccgggcggg agcgcccaag gctggagccc gctcgccggc agtgccgtct
     1741 tttccgtggc aggcggtgca ggtccggtta taaatttcct cgccttccgc gaccgcctgc
     1801 tgattgccgc tgaaagggtt ggtgggaccg gcaggttgaa cctggttgct gcggttcctt
     1861 tgcccgtata cgggaagggc ggcggccgac aacaaggcga cggccagaac ctggagagag
     1921 cggcggccgc aacttcccgt gctcttggta agcttcataa ggcgactccc atacctatgg
     1981 tccggtggcg attcctttcc ggcagagttt cctatcttct ttttttctct cagcttgcgt
     2041 ctcatttgcc atcgggaagc ccaaaagcga tgacgttggt gccggaaccg atggccacgt
     2101 attccttacc gtctatgcta tacgccatcg gcgacgctgt aatcaggtcg ccggtattaa
     2161 agtgccagag gacttttccc gtgcgcgcat cgagggctgt aaactgccct tgctgctcgc
     2221 cggcaaacag cacgcccccc gcagtggaaa gcagacccgg tccgtagtga tgggatccta
     2281 tttcctcgta gtcccagacc tttttgcccg tcgtcaaatc cagagctcgg acgaaaagct
     2341 gccagcgttc ctgctgatcg gggctctcca tgtatccagt gccgctgttg aagccggcgc
     2401 cccccggacg aaagttgtcg ctggccttgt aattgatgcc gcagccttct tgcgctatca
     2461 ggtagaagta gtgggtatcg gggttgtaag cgggagaagg ccagttcgtc gctccagccg
     2521 tggacgggca taccttgttg ccttgcacgg tcggatctgt tcccggcacg acaaccggcc
     2581 ggccgtcctc agtcaagccg ctcgaccagg tgaccgcgtt gacaaaaggc gtgccaaata
     2641 gaaactttcc gttggtgcgg tcgagaatgt agtagtagcc gttgcggttc gcctggatca
     2701 gcagcttgcg catctgcccc ttgaattccg cgtcgacaag aacggggatt tcaactgcgt
     2761 cccaatcgtg gacgtcgtgc ggagtaaatt ggaaatacca tttgagcttt ccggtatccg
     2821 ggtcgagggc gaccatgcaa tccgtgtaga gattgtcgcc cttgcggatc tcgcccacga
     2881 aatcgacgcc gggctggccc gtgccccaat aaagcaaatt caaatcagga tcgtagctgc
     2941 cggtcagcca cgtcgggccg cctccgagtt tccaggagtc cccttcccat gtttcactgc
     3001 ccggttcgcc gggcttcggt atgacgttcc aggtccagag gtgctttccc gtttcggcgt
     3061 cataagcctg gacaaagccg ccggtccgcc cgctgccgcc gacatagact ttgcctttca
     3121 ctatcagagg cgcgccgccc tgcaagcgca ccccgccggg cgcttttgtc tcccagacca
     3181 gcgcgccggt tcgtgcatcc agcgcgatca cgcaggagtc ggccgtggtc acgtaaaccc
     3241 ggtggccgta aaccgccaca ccgcgctgcc agccgcgcgt cggcggctgt ctttggtatt
     3301 gccagatcag ccggccggtc cgcccgtcga gggcgtccac ccgattgaat tgcgaaatgt
     3361 acatcacgcc attcaccacg atcgggactg cttcaaaatc gactgctccc gacatgtgat
     3421 atatccattt cgcttgcagg tttgcgacat tgcttgtgtt gatctggttc aatagcgtgt
     3481 gccgctgtgc gttgtaagag cccgagtacg tcagccagtt gttgggattg ggcttcaaca
     3541 aatcttccga agtgacttgg gccgcggctg tgcacgccgc cgcccaaagc aaacaaacca
     3601 gcaataattt cgttttcatt tcgacagaac gctctccccc tcccgacgcc tgtcaccgcg
     3661 ccagccgtgg actgcatggc gccgccgcgc ctcaaaaacc gaactcagca tgcgaggcat
     3721 ccatagtgta tctcagatga ttcacgaact tacaagggac aggagtgaca aatacaagct
     3781 taggggcgtt cggaaatgtg aataaattgt ggagagcgag cccctgacgg cgagggcggt
     3841 tgccggtttc gcctctgctt ctctttggga cccgttgact tacaaattta gccgggaaac
     3901 accagccctg cccgcccgtt tgcagttccc ttgaaaatgg aggagatgaa aacggagcag
     3961 aaccgggatc acgaaccggc agtgctgcgg tctctggcag agtcgcagac cgaagcgacg
     4021 cgtagaagga aagcctttgt gaattcgatg cacagcgaac cgttagagcg cctctttgtg
     4081 tgaccagcgc cggttggccg gggcatctaa ctagtaccca ttcagctcgc gggtaaaggt
     4141 catcaaaaga tcatgaatct tccggccttc tccgtgcaaa ggatccgccg cccgcacaat
     4201 tcccagtctt ttggggaaag tctcggcgcg ggcctgtgcg ttgggctgcg gatctatctc
     4261 gatctggaaa agcgcctgct ccgtatgcct ccggtcgccg tgaagcaaag tatcgaatgc
     4321 ggcgcactgg ctgcaagaac cgtttttgtt gatcaatccg cagcggcttt cgtaccggtc
     4381 gatcatcgcc tgccgggcct gttccaaacg gaatcgaacc agttttcccg aaattcccag
     4441 cgtttcggcc gcttcttcga gtgtgaaccc atgcacgtcg attaaaagca gcgctgcgtt
     4501 ctcttccggc ggaagagtgc ggcccatcga cgtgaagcag aaatcgatat gctcgcggat
     4561 ttcatatgtg gagtgtgtgc cggaccggtg cagtttttgc agttttcgcc gctgcccggg
     4621 atcctccccc gcgatctggc cggcgcggat cacggcatcc ggatcccact gccgcttgcc
     4681 ctgtagatgc gcgagtccgg cttctgccgc caagcgaaac atccacgtgc gaaacgaaga
     4741 atcgcctggg aaatcggcga tgctttcgag agccttcagc gccgtctcct gcatcagatc
     4801 ctcggcatcc tctcgcacgg caagcaaccg atacagaaac gcgccgagcg gcttttcgag
     4861 cggctcatag agctcggaga acgcttcgcg atcgccttgt ttggcgcggg cgatcaaggc
     4921 cgcctggtcc ggcagcccaa tgctccgaga ggcgttagag acacccatat ctttattcta
     4981 cccggcagct tccccaggcc gcgccgcggc tttccaggaa gtccggcgct ggcggcattc
     5041 acctgctgcc gagaggcggc gccccacgct cgcaggggaa cgccgggacg cggatttcgc
     5101 gacccggcgg aatcagctat ggcggtttca gttccagtaa cttcggtcca ggctgcggta
     5161 ttgcaccgcc tcggccaggt gcttggcgga gatcatctcg gaccggtcca gatcggcgat
     5221 cgtgcgcgcc agcttcaaga cacggtcgtg cgcgcgcgcc gaaaggccca tccgctgcac
     5281 cgccaactcc aatgttctct caccggcttc atcgagtgcg caatatttgc gaagcaggcg
     5341 cgtcggcaac tgggcgttgt aatacccgcg cgccttctga agctcccggg ctcgctccac
     5401 ccgcccgcga atttcggccg agccttcggt ggcggcatcg gcccgcatct ctttgtactt
     5461 cacggccgga acttctatgt gaatgtcgat acggtccagc aaggggccgg aaattttgcc
     5521 aagataccgc tggatgatcg ggggcgtgca gcggcactcg cgcgtggggt cgccgtagta
     5581 gccgcaagga caggggttca tcgccgcaac cagcatgaac cgcgaaggga acgtcagcgt
     5641 catgttggag cgcgcgatcg ttacgctgcc ctcttcgagc ggctgccgga gcacctccag
     5701 cgtattgcgg gcgaactccg gcagttcgtc caaaaacaat acgccgttgt gcgccaggct
     5761 cacctcaccg ggccgtgcga cgcccatgcc gcctccgatc aggccagcgt cagaaacatt
     5821 atggtggggc gcccgaaacg ggcgctcttg caggattcct tcctctggat tcagtacgcc
     5881 ggctacgctg tgcaccttcg aggtttcgag cgcctcctga aacgtcagtt ccggcaaaat
     5941 gcccgccagc cgtttggcca gcatggtttt gcctgaaccc ggcggaccga tcatcagcag
     6001 gttgtgcgcg cccgccgcgg ctacctccag cgcccgcttc gtcgcttgct gccctttcac
     6061 gtcgcgaaaa tcgaggccgg acccattcgc acgcaatcgg gcgccggcgg cgcgggtgag
     6121 ggtagggaag cggtcccatt tcccgatccg ctcgaccacc tcgcccaaac tgcccaggcc
     6181 atgcaccgag atgccttcca ctaccgctgc ttccggtccg ttttccttcg gcaaaaccag
     6241 gtggcggatg cccatttgtt gcgcgcatac cgcaatcgag agggcgccgc gaatcgggcg
     6301 caaaccgcca tcgagcgaca gctccccgac cagcaggcaa tcttgcaagc tctgtggcgc
     6361 cagggtgccc atcgcgccta agattcctac cgccattggc agatcaaatc ccgctccctc
     6421 tttccggacg ttcgccgggg ctaggttgat ggtgactgat ttattcggat acccgaagcc
     6481 gcaattgatc agtgacgatt tgacccgttc gcggctttcg cggaccgcca catccggcag
     6541 gccgaccgta atgaactccc gcgcggagcc ggcatacata tccacctcca cttcgattag
     6601 gtgcgcatca atgccgtaaa ccgcggcgct gcgagtcttg aatagggcca tggcgcccct
     6661 tccttgccgc ttccgcaggg tcagtcctta tcgcgcgaga caaaccggac gtagtaacca
     6721 gcccagacgg aaagcaatgc gattaacaat ataattgcta ctattttttg cacattaccg
     6781 caaaattata ctcccctttt ccgttcgaga ggcaataaac caaaacaaca aatggcacgc
     6841 ttccagttga cgttttcgca cccgagccgg tcctgcatgg tttttcggaa cttatcgacc
     6901 cgctcctctc ggctgctcgg agaaccgcga tccagtcacc gcagattcgg tgctgcgctc
     6961 gaaaaaccaa acggccgccg gggccggcga ggttgaagga cctgcggcgg gttcgcctgc
     7021 cgcctgacag tttgctcccc gctgtttcta cagcaactcg atacgccttc ttactccccg
     7081 ccatttcgtc aacgggaatc ctgcgttccc gaaggctgtc ggctcccccg gcgtcttctc
     7141 gaaaaattcg tgcaaggttt ttgcttccaa ccgttcttat taagtaggag ggcagtacta
     7201 ccctctgtgg aggcgaacaa tgattcgaaa aggtgtcgct cgagagcgga tcggccgtac
     7261 ggtgctcgga agaaaaacaa tcgcgccggt tctggccgta cttttcagcc tttccgttct
     7321 gccggccgcc gatctgccgg ttcgcaaggt cactctgtac aaagacggca tcggcttttt
     7381 cgagcgtgcc ggacgggtgc cggccggcga gacggcccgg ctggagttca cggccgatca
     7441 gatgaatgac attctcaaat ccttaaccat tgaggagcgc ggcggaggcc gggtcatcgg
     7501 cgtccgttat gactccagtg agccgttagc gcgcaagctc caggaattcc cattcgccat
     7561 atccagcggg caagcggtcc ccgcaattct cgaccagatg aaaggggcca gggtggaatt
     7621 gcacttcggg tcggcgggcg gggcggagcg tgtccagggc attattgtca gcgcgcgctc
     7681 ggctgccgcc tcggagcgcc aggccgaaaa acaagaactc atcctgctgc tcgattcggg
     7741 cgacctgcgc gcggtggatt tgctcgccgc ttccggcata cgctttatgg atcccaagct
     7801 acaggcacaa ttccaggagt atctgatcgc tcttggccag tcgcgcaacc gtgacaagcg
     7861 cggcctcctg atcgagtctt ccgccgcggg cccgcgcgat ctgatcgccg attacatcat
     7921 tcccgccccc gcttggaaga ccagttaccg gctgattttc gattccaacg ccgagccgat
     7981 gctggaaggc tgggctattg tggacaacct cactggagag gattggacga acatctcgct
     8041 ttcgctcgtt tccgggcttc cggtctcgtt tgttaccagg ctctacgagc cgcgctacgt
     8101 agcgcggccg gaggctgagc tgcccgggga tcgcgcccaa cggcccattc tccatgcggc
     8161 agctatggaa gagctgcaaa gtgcggcgaa cagggccgcc gctgccccgg tccttcgcgg
     8221 cgcaccggct gtgcaagccg gggtaggggg cggcgtggtc ggaggctggc cggatcgcga
     8281 attccgcaag gagcgggatg cgaagccttt ggagcgagcg gaatttgctt cttccctggc
     8341 gcagaccgcc ggcgggcgcg agttgggcga tctgttcgaa taccggatcg ggcaccccgt
     8401 gacggttcgt aaggaggaat cggctatgct gccttttctc cagcagagga tcaaggcgga
     8461 gcgcctcctg gtgtattcgg agtcctatgg ctctcagcat cctctggctg cggtacaaat
     8521 cacgaactcg acgggaacca ccctcgatgg aggggcgatt acggtcatcg acgccggggc
     8581 ctacgcgggc gaatccttga tggaaacctt gaaggccggc gataagcggc tggtgagcta
     8641 tgcggtggat ttgggcgccc gtgtgacgac cgcctttgat tcccaaagcc agttgctgcg
     8701 ggaatttcat ttccgccgcg gcgtgctgac tacgcgcacg gcgatccagg aaaccaagac
     8761 atttactgtg cggaacgtgg attcccgctc caaaaccctt ctgatcgaaa ccccggttcg
     8821 ccctgaattt aagctgctcg acatccagcc gctggagact acaccgaacg cccaccgctt
     8881 tgccctcaag ctgggcccga atacgactca gagattcccg gtaagggaag agcgggtcat
     8941 cgaaaactca atcgccatct cgagcctgac gccggatgtg ctgatgacct atgtgcagaa
     9001 ccggaatctg gaggatgcca gccgaaagaa gctcgagccg cttctgactc tcaaacagca
     9061 gcttgcctcc gcgcaggccg aagcccagcg ggtcgaaaag gagatccagg acttgtttca
     9121 agatcagcag cgcctccgcc agaacatcga cagcctgaac cgggtcaacg gccagcagca
     9181 gcgcgtgcag gagtacgccc agcgcctcgc tagccaggag agccagctcg ccggcctgcg
     9241 cgaccgccag tctgaattgc agcggcgccg cgcgagcctc gagcagcaga tcagcaaact
     9301 ggtcgacgcc ttggagtttt aacagacggc gatgttaagc gcgaagcgca gcgggtcttc
     9361 acctccgccg cggcggcacg ggcaggatag acagcgtgac ctgcacaacg gacgcgggct
     9421 cgcccgcaag ggccggagaa agctgaatgg cagcatgata ggccccgatc cccagccccg
     9481 cgggtcggac caacacggaa atttcagccg gcgtggagcc ggcgctttgg gaaatgccga
     9541 gccacgccgg agcttccgtg cgcgcactcc agcgaatcgg cgccggacct gcactcaaaa
     9601 ttttgagggt ttgcggggcc gggtcccgcg ctccaaagaa agtcacgaac cgcaattcgg
     9661 ttgacgaggt gttgagaaga gggcgatagg ggaacgcccc gagatcgggc gaggcggcca
     9721 tcggggccgg cgggattttc gaagacggaa cgtcggtgag gcctctgccg atcacaggtg
     9781 agatggcgcg cagccagaac cagccgtttt cggcggaaac aaatttcgga tcggtggtga
     9841 ggccgtggct ctccagcccg gtcttctctt gcaatgcctc gagactggtg agatcggccc
     9901 cactccagct cgccagaggt cctccgaccg cccggtacca gtcgttatag tcggccagga
     9961 ggccaggtcc aggatccacg gcgcgcagcg gataaagaaa cggggcggcg ctggcctcga
    10021 ctgtaatgtt gttaaggatt cggattccgc tcggagcggc atccgcgcct tgggctgttc
    10081 ctgccgaaac gctcgccgca taaaggagca atccgttgga cctgtccgcg taaaacgtat
    10141 tgttgtaaat cagcgcgccg cccacgttgc ccgccagacg cagttcggcg tccatgccgc
    10201 tgcccgcgcg agcctggtta ttctgctgaa acagattgaa ccggataatg ttgctgcgtg
    10261 acggagagac gccgtttttc ccgtcgacgg aaacaccgtt gccgccgttc caggcgaaca
    10321 tgttttcttc gatggtgttc tcgctggggg ccgaagggcc ttccgagccg agcaaatcag
    10381 actccaggat gaggccgtgg tagccgttgc caaatatgcg gttgccgcgt acggtctcgc
    10441 gattgccgcg caaaatgaat ccgggaccga tcaacctttg tccatagccc gcttcttgcg
    10501 cccagtccgg ac
//
LOCUS       DASREK010000301         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1131324, whole genome shotgun sequence.
ACCESSION   DASREK010000301 DASREK010000000
VERSION     DASREK010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131324"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFA54_16365"
     CDS             complement(<1..717)
                     /locus_tag="VFA54_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HZS52446.1"
                     /translation="MRAPRPLIDRQTLEKLERLALRWQRSFTGLFGGHNPSRFPGPGQ
                     EFLDHRHFHHGDDLRSVNWLAYMRLERLFLKMFQIEPRVPVVILIDTSESMACGASDG
                     QGGEAKFAYACRLAAMLCYVGLVRLDTIVLQPFSAGLAEDYRAQGGRHRFAPAMNFIM
                     ELETGGRSDFHTCVRQLISSGPGRGLVVIISDFLDDGEIGCEAPIQHLADFGNELLLV
                     HVAGADDRMPSWRGELELVDA"
     gene            complement(722..1717)
                     /locus_tag="VFA54_16370"
     CDS             complement(722..1717)
                     /locus_tag="VFA54_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZS52447.1"
                     /translation="MPSDQSLEKLSHQIQLVRNEIAKVIVGHQEVVDGVIIALLAGGH
                     VLLEGVPGLGKTTLLRTLGRALQLRYSRIQFTPDLMPADIVGSMVMETDDRGVKNLRF
                     QPGPIFSHLVLADEINRATPKTQSALLEAMQERTVTSGTVTHELEEPFLVMATQNPLE
                     MEGTYPLPEAQLDRFLMKILVGYPSRAELDIIVQRTLASTQPQVERVVDRDQILAIRR
                     AAMDVLAAPHVREFASHLVLATQPEQAEAHPLAKKYVRYGSSPRGAQALLQSARVLAL
                     MRGRWNVSIDDLRYLALQCLRHRLILNFDAHAEGKTTDALLTEILNTIPVTTLAQ"
     gene            complement(1719..>2866)
                     /locus_tag="VFA54_16375"
     CDS             complement(1719..>2866)
                     /locus_tag="VFA54_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52448.1"
                     /translation="PFTVPHSLYGVASVFLLASALCALRYFLEKPFRVNRPLPEVIAR
                     AIRNDQTAREKGRRPNPPQEGKHSPEERAKAYATDNAQEQNQVKPEQAKDISEPQASG
                     AAPADQKSDQQSEEQKAQAEQQDPPQTSGFGDPMQSAENYPIQSYEDMLERDGKSGAD
                     KAEGKESKNGNNGQNGKGAGDPDSNSSNSGLFSKLKEAMSNMLSRLQQKSAGDAGKQQ
                     QGAQGEQSAGNEPKDGAGQANSASNGQPKAGSQDGAEAQAVSSDAKDASAQKSSNKNT
                     GNTSDKGSHSAGSGAGREDGDKQVLEAQQQQAMGKLSELYGRRAANVTGEVTVEAQSG
                     KQTLRTPQSQKQAYHADSGGEVSRDEIPLAYQPFIKEYFNKIREAKK"
BASE COUNT          492 a          895 c          857 g          622 t
ORIGIN      
        1 ggcatccacc agctccagct cgcctcgcca ggacggcatg cggtcgtcgg cgccggcgac
       61 atgcaccagc agcaattcat tgccaaaatc agccagatgc tggatcggcg cctcgcagcc
      121 gatttcgccg tcgtcgagga agtccgaaat gatcaccaca aggccgcggc caggaccgga
      181 agaaatgagc tggcgcacgc aggtatggaa gtccgatcgc cctccggttt cgagctccat
      241 gataaagttc atcgcaggag caaacctgtg gcgcccgccc tgcgcccggt aatcctcggc
      301 caatcctgcc gaaaagggct gcaacacaat ggtatcgagc cggaccagcc ccacgtaaca
      361 gagcatcgcc gccagccggc aagcataggc gaatttcgcc tctccgccct gcccgtcaga
      421 ggctccgcaa gccatcgatt cgctggtatc gatcaggatg accaccggga cccgcggctc
      481 gatctgaaac atttttaaaa acaggcgctc cagccgcatg taggccagcc agttcacgct
      541 gcggaggtcg tcgccatgat ggaaatgccg gtgatctaag aattcctgcc cgggccccgg
      601 aaaccgggat gggttgtgac cgccgaataa gcccgtaaag gaccgttgcc agcgcaacgc
      661 aagccgctcc agcttctcca gcgtttgccg gtcgatcagc ggtcggggag cgcgcatact
      721 tctattgtgc cagcgtcgtt acggggatgg tgttaagaat ctccgtgaga agagcatcgg
      781 tggtcttgcc ttcggcgtgc gcgtcgaagt tcaggatcag acgatgccgc aggcattgca
      841 aagcgaggta gcgcagatcg tcgatgctta cgttccagcg cccgcgcatt aacgccaaca
      901 cgcgggcgct ttgcagcagc gcctgcgctc cgcggggaga gctgccgtag cgcacatact
      961 tcttggccag ggggtgcgcc tccgcctgtt ccggctgcgt ggcgagaacc agatgagatg
     1021 cgaattcgcg aacgtgcggc gccgccagca catccatagc agcccgccgg atggccagaa
     1081 tctgatcccg gtctaccacc cgttccactt gcggctgggt ggaagccagc gtgcgctgca
     1141 cgatgatatc cagctcggcg cggctcgggt acccaaccag aattttcatc agaaagcggt
     1201 ccaactgagc ctcgggcaac ggataggtgc cctccatttc cagagggttt tgtgtcgcca
     1261 tcacgaggaa cggttcctcc aactcgtgcg tcaccgtccc gctggtaacc gttcgctcct
     1321 gcatggcttc cagcagcgcc gattgcgttt tgggcgtggc tcgattgatc tcgtcggcga
     1381 gaacgagatg agagaagatc ggcccgggtt ggaaacgcag atttttcacg ccgcggtcgt
     1441 cggtttccat gaccatgctg cccacgatgt cggccggcat gagatcgggc gtgaactgaa
     1501 tccgcgagta ccgcagttgc agcgccctcc ccagcgtccg caggagtgtg gttttgccta
     1561 aaccaggcac tccttctaac agcacatgac cgccggccag cagcgcaatg atcacgccgt
     1621 caaccacttc ctgatggccg acgatcactt tggcgatttc gtttcggacg agttgaatct
     1681 ggtgcgaaag cttctctaga gattgatcgg agggcacgtt attttttagc ttcccggatc
     1741 ttgttgaagt attccttgat gaaaggctga taggccaagg ggatctcgtc ccggctcact
     1801 tctccgccag aatcggcatg atacgcctgt ttctgcgact gaggagtccg aagcgtctgc
     1861 ttgccggact gcgcctccac ggtcacctcg ccagtgacat tcgccgcgcg gcgtccgtat
     1921 agctcgctca gtttgcccat cgcttgctgt tgctgggctt cgagcacctg cttatcgccg
     1981 tcctcgcgcc cggcgccgct gccggccgaa tgagatccct tatccgacgt attcccggtg
     2041 tttttgttgg acgacttttg cgcgctggcg tcctttgcgt cgctcgaaac ggcctgcgcc
     2101 tcggcgccat cctgcgaacc cgctttgggc tggccgttgc tggcgctgtt agcctgcccc
     2161 gcgccgtctt tcggctcgtt gccggcgctc tgctcgccct gagctccttg ttgctgcttg
     2221 cccgcatcgc cggcggattt ctgttgcagc cgggaaagca tgttgctcat ggcctctttc
     2281 agcttgctga aaaggccgct gttcgaagag ttcgaatcgg gatcgcccgc gccctttccg
     2341 ttttggccgt tattcccgtt tttgctttcc ttcccctcgg ctttatcagc tcccgacttg
     2401 ccgtcgcgct ccagcatgtc ctcataggat tgaatcgggt agttctcggc gctttgcata
     2461 ggatcgccaa accccgaggt ctggggaggg tcctgctgtt cggcctgcgc cttttgctct
     2521 tcgctctgct ggtcgctttt ctgatcggcg ggcgccgctc cggaggcctg cggttcggaa
     2581 atgtcttttg cctgctccgg cttgacttgg ttttgctcct gcgcgttatc ggtcgcgtag
     2641 gccttggcgc gctcctcggg cgaatgtttc ccttcctggg gcgggttggg acggcggccc
     2701 ttctcccggg ccgtctggtc gttgcgaatg gctcgcgcga tcacttccgg cagcggccgg
     2761 ttgacccgga agggcttctc cagaaagtac cgcagcgcgc agagggcgga agccagtaga
     2821 aatacggagg cgaccccgta aagcgaatgg ggtacagtga aaggga
//
LOCUS       DASREK010000302         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1962510, whole genome shotgun sequence.
ACCESSION   DASREK010000302 DASREK010000000
VERSION     DASREK010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962510"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1792)
                     /gene="bshC"
                     /locus_tag="VFA54_16380"
     CDS             complement(188..1792)
                     /gene="bshC"
                     /locus_tag="VFA54_16380"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HZS52449.1"
                     /translation="MTCRCIRHTELPHTSKLFADFIYDFDRVRKFYPYPPYAAESFAA
                     AAGAIRMDPRRRAQLVEALAEENQNAGEAVLNNLDRLSRLDTLAVATGQQAGLYTGPI
                     YTIYKALTAVRLAADLTERGTPAVPIFWLATEDHDLAEVNHSWVFDVSGQPVRLQASA
                     TAQPNQPVGTIRLTGNDGGKLREALAALPFGGEVAELALEAYADGVTFAEGFKRLLAR
                     LLSRYGVLLLDPQSPALRRLAAPVIARAIREAPDLTAALLERGKDLETAGYHAQVHME
                     EKTSLFFLIENGTRTHLTRSSQGYTENGNLLTPEQLLVRLDGNPEDFSPNALLRPVVQ
                     DYLLPTVAYIGGPAELAYLAQAEVLYRRVLGRMPVAAPRAGFTILDARAEKLMKRYGL
                     DLGDVLHGPAALEQRIALTLAPPELQAAFHAATQEVESALDGLGAKLSAFDPTLAAAL
                     ENSRRKIRYQLAKIQGKAAREAMRRTERARSDAAYLINLILPEKTLQERLYSVLPFLA
                     RHGLGLLDDIAKAIQFGCPDHQILSV"
     gene            1881..2273
                     /locus_tag="VFA54_16385"
     CDS             1881..2273
                     /locus_tag="VFA54_16385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZS52450.1"
                     /translation="MLQKLKLQTTARTLMLDVTGQVQKAVERSGVRSGLACIFTPHTT
                     AGVVINEHDDPDVARDILSTLEKLVPRQGDYRHYEENSDSHIKSALAGCSQTVIIEEG
                     RLLLGTWQGVFFCEFDGPRQRELWIKII"
     gene            2378..3520
                     /gene="hemW"
                     /locus_tag="VFA54_16390"
     CDS             2378..3520
                     /gene="hemW"
                     /locus_tag="VFA54_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZS52451.1"
                     /translation="MLAPDADVNKNKGIGVYISFPFCQQKCTYCNFSSGVFPAYLKER
                     YRAALEAEIADEPWPEPPETVYLGGGTPSLLDPDELGRILGRLPGVPWKEATIEAAPG
                     TVTRERAAAWRSLGISRVSLGVQSFVAQEIRATGRTHTATQVLLEIEALRREGLNNIN
                     VDLIAGLPYQTDQSWRESLDWIEALAPPHVSVYMLEADEASRLGREILSGGTRYGAGA
                     VPGDDQVAALYSEAVARLATLGIHRYEISNFARPGHESLHNLKYWRREPYRGFGAGAH
                     SFDGLRRWSNVESAWEYVKRWAAGASPVDHIELLSDTQSMEERFFVGLRQTAGIAPAE
                     REWARFAEPIRRLIDSGLLERDEERLRLTGRGVLLSNLVFEEFVNP"
     gene            3517..4611
                     /locus_tag="VFA54_16395"
     CDS             3517..4611
                     /locus_tag="VFA54_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HZS52452.1"
                     /translation="MSARSEYEFARAVEAGGAEEPVVDLRSDTVTKPTAEMRQAMAEA
                     EVGDDVYREDPTVNRLEEQAAAILDKEAALFVPSGTMGNQIAVKVHTEPGQEVICEER
                     SHIFNFEMGMMAAFSGCQVRTVATKNGIMTWDQIREKLRPGIYYYSRSGLITLENTHN
                     MAGGTVYSLEQMQEICDRAHEAGLPVHLDGARIFNAAVCLGRPVRELAAPFDSVMFCL
                     SKGLGAPVGSLLAGRRAFIDKALRVRKMLGGGMRQAGVLAAAGLVALEKHPVRLAEDH
                     RNARFLAESLRKVPGIAVDLRNVQTNIVIFDVSGAGLSASEFSARLKALGVLINPINE
                     RQMRIVTHSDVDHAACRRAAEAVTAVAVGP"
     gene            4729..4947
                     /locus_tag="VFA54_16400"
     CDS             4729..4947
                     /locus_tag="VFA54_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52453.1"
                     /translation="MKRIASLVLGMSLVLGTAAFAAAQAEQSKDQSKTATANQTEKKK
                     HKRNKKQAKAANTTSSGSSTASTSKPAK"
BASE COUNT          995 a         1441 c         1633 g          965 t
ORIGIN      
        1 ctcgttgtca tcctcgcgtt gggtctgctg gcgatatcga gactttgaag gctttcccag
       61 aaccagccag gagggccctg ccgccgttcc ttttggccgc tttctgccgg cgcgcgaggc
      121 tcttccttca tagggcggca gtccagatct caaaaagccg gtcacaggac gcctctgcgg
      181 ctgaatctca gacgctaagg atttgatgat cgggacagcc gaactggatg gccttggcaa
      241 tgtcatcgag cagacccagt ccgtgccggg cgaggaaggg caggaccgaa tagagccgtt
      301 cttgcaaagt cttttctgga aggatgagat tgatcagata agcggcatcg gagcgggcgc
      361 gctcggtccg gcgcatggct tcgcgcgccg ccttgccctg gattttagcg agttggtagc
      421 gaatttttct acgagagttt tcgagagcgg cggcgagggt cgggtcgaag gccgacagtt
      481 tggcgccgag tccatccaat gcgctttcga cttcctgggt ggcagcgtga aatgcagctt
      541 gcagttcggg aggcgccagc gtgagggcga tgcgctgctc caatgccgcc ggcccatgca
      601 gaacgtcgcc cagatcgagg ccgtagcgct tcatcagctt ctcggcgcgt gcgtccagaa
      661 tggtgaatcc agcccgagga gcggccactg gcatccgtcc gaggacgcgc cggtacagaa
      721 cctcggcctg agccaaatat gcgagctcgg caggtccgcc gatgtaggcc acggtgggca
      781 gcagatagtc ctggactacg ggccggagca gggcgttggg tgagaaatcc tccggattgc
      841 catcgagccg cacgaggagc tgttccggcg tcaataaatt tccgttttcg gtgtagccct
      901 ggctggaccg ggtcaggtga gtccgcgttc cgttttcgat aaggaaaaac agggaggtct
      961 tctcctccat gtggacttgc gcgtgatacc cggccgtctc cagatccttg cctcgctcca
     1021 aaagggccgc cgtgagatcc ggcgcctccc ggatcgcccg cgctatgaca ggcgctgcca
     1081 ggcggcgtaa agcgggcgac tgcgggtcaa gcagtagtac gccatagcgg gagagcaggc
     1141 gggccaggag gcgcttaaac ccctcggcaa aggttacgcc atctgcatac gcttcgaggg
     1201 ccagctctgc gacctcgccg ccgaagggta gagccgccag cgcttcccgc agcttgccgc
     1261 cgtcattgcc ggtgagccgg atcgtgccga ctggctggtt tggctgggca gtagcggagg
     1321 cttgcagacg gaccggctgg ccggaaacgt cgaataccca gctatggttt acctcggcca
     1381 gatcatggtc ttcggttgcc agccagaaga tgggcacggc cggcgtgccc cgctcggtga
     1441 ggtcggcggc gagccgaacc gctgtcaggg ccttgtagat cgtgtagatc gggccggtgt
     1501 aaaggccggc ttgctgccct gtggctaccg ccagcgtatc cagccggctc aggcggtcca
     1561 gattattgag caccgcctct ccggcgtttt gattttcttc tgcgagagct tccaccaact
     1621 gcgcccgccg ccggggatcc atccggatcg cgccggccgc tgcggcaaac gactcagcgg
     1681 cgtacggcgg atagggataa aacttccgga cgcgatcgaa atcgtaaatg aaatccgcga
     1741 acaatttcga ggtgtggggc agttcggtgt ggcggataca gcgacaggtc atgggatccg
     1801 gaaaatgaat tccaagctct caagtacacc aacgccagag atgtctcaac aggcgcgcct
     1861 tcgatagaat agcagaaatc atgctccaga aattgaagct gcaaacgacc gcgcggacct
     1921 tgatgctgga cgtgaccggc caagtgcaaa aagcggtcga acgctcgggc gtccgcagtg
     1981 gcctggcgtg catttttacc ccgcacacca ctgccggggt ggtcattaac gagcatgacg
     2041 atccggatgt cgcccgcgac atcctgtcta cactcgaaaa actggttccg cgccaagggg
     2101 attatcggca ctacgaggag aactctgact cgcacatcaa gtccgcgctg gccggctgct
     2161 cacaaactgt tatcatcgaa gaggggcggc tgctgctcgg aacctggcag ggcgtattct
     2221 tctgtgagtt tgacggcccc cggcagcggg agctctggat taagatcatc tgattctccc
     2281 ctatgggggc gagcgtggaa tccacaatcc cgggcatgtc ggaaggctgt ccgggaccgg
     2341 atccgcggtt ccctgaccgg gtggcggggc cccagtgatg ctggctccag acgctgacgt
     2401 caacaagaac aagggtattg gcgtatacat ttccttccct ttttgccaac agaagtgcac
     2461 gtactgcaac ttttcttccg gcgtgtttcc ggcgtatctg aaagaacgat accgggccgc
     2521 cttggaagct gagattgcgg atgagccgtg gcctgagccg ccggagactg tatatttggg
     2581 cggcgggact ccaagcctgc ttgatcctga cgagttggga aggattttgg gacgcctgcc
     2641 aggcgtccct tggaaggagg cgacgatcga ggccgccccc ggcaccgtga ctcgcgagag
     2701 ggcggcagcc tggcgctcgc tgggaatcag ccgcgtcagc ttaggcgtgc agtcttttgt
     2761 ggcgcaggaa atcagggcaa ccggccggac ccacaccgcg acgcaggtgc tactggagat
     2821 cgaggcgctg cgcagagagg gcctcaacaa catcaatgtg gatttgattg ccggcctgcc
     2881 ctaccagacg gaccagagct ggcgggaatc cctagactgg atcgaggcgc tggctccgcc
     2941 gcacgtatcg gtctacatgc tggaagccga cgaggccagc cgcctggggc gcgagatctt
     3001 gagcggcgga acgcgctacg gcgccggcgc cgttcccggg gatgaccagg tcgccgccct
     3061 ctactcggaa gccgtggccc gcctcgccac gctgggtatt caccgttacg agatttctaa
     3121 ttttgcccgg ccggggcatg aatcgctgca caacctgaag tactggcgcc gcgagcccta
     3181 tcggggcttt ggggcaggcg cgcattcgtt tgacggcttg cggcgctggt cgaacgtgga
     3241 atcggcgtgg gagtatgtca agagatgggc agccggggcc tcgcccgtgg atcacatcga
     3301 gctgctcagt gatacacagt cgatggaaga gcgttttttc gtcggactgc gccagacggc
     3361 tgggattgcg ccggccgagc gggaatgggc gcggtttgcc gagccgatcc ggcgtttgat
     3421 cgacagcggc ctgcttgagc gcgacgaaga acgtctccgg ctgactggcc gcggagtgtt
     3481 gttgtccaac cttgtattcg aggagttcgt aaatccatga gcgcgagatc ggaatacgaa
     3541 ttcgccaggg cggttgaggc ggggggagca gaggagccgg tcgtggatct tcgcagcgac
     3601 accgtgacca agcccaccgc ggagatgcgc caggcgatgg ctgaggcgga agtaggggac
     3661 gatgtttacc gcgaggatcc cacagtgaac cggctggagg agcaagccgc cgccatactg
     3721 gacaaagagg ccgcgctgtt tgtcccctcc ggcaccatgg gaaaccagat cgctgtgaaa
     3781 gtgcacaccg agccggggca ggaagtgatt tgcgaagagc gctcccatat cttcaatttc
     3841 gaaatgggga tgatggccgc cttctcggga tgccaggtgc ggaccgtggc cacgaaaaac
     3901 ggcatcatga cgtgggacca gatacgtgaa aaactgcgtc ccggcatcta ctactattcg
     3961 cggagcggcc tcatcactct ggaaaacacg cacaatatgg ccggcggcac ggtttattcc
     4021 ctggagcaga tgcaggaaat ttgcgaccgc gcgcatgaag ccgggctccc agttcacctt
     4081 gacggagccc gcatcttcaa tgccgcggtc tgtctcggcc ggccggtaag ggaactggcg
     4141 gctcccttcg attcggtaat gttttgtctt tccaaaggac tgggagcgcc ggttggttct
     4201 ctgcttgccg ggcggcgcgc cttcatcgat aaggcgctcc gcgtccgaaa aatgctcgga
     4261 ggggggatgc ggcaggcagg cgttctggca gcagccgggt tggtcgctct ggagaagcat
     4321 cctgtcaggc ttgccgaaga tcaccgtaac gcgcgattcc tggctgaatc gctgcggaaa
     4381 gtgccgggaa tcgccgtcga tctccgcaat gtacagacca atattgtcat ttttgacgtc
     4441 tcgggcgccg ggctttcggc aagcgaattt tcggcgagat tgaaagcgct tggcgtattg
     4501 attaatccca tcaacgagcg ccagatgcgt attgtcactc actctgacgt ggaccacgcg
     4561 gcctgccggc gggcggctga ggcggtgacg gcagtggctg tggggccata aatccccttt
     4621 gccggcggtc attaataaag tttaatttct gcttaagcca gcgttaatcg tctgcttcgt
     4681 ctcactaatt ggaggcggca aaagccgccc cgacggagga gataccgaat gaaacggatt
     4741 gctagtttag tattgggcat gtcgctggtt ttgggaaccg ctgctttcgc ggcggctcag
     4801 gcggagcaga gcaaggacca aagcaagaca gctaccgcca atcagacaga aaagaagaag
     4861 cacaagagga acaagaagca ggccaaggcc gcaaacacca cttccagcgg atcttccact
     4921 gcttcgacat ccaaaccggc aaagtagccc gaagggtgtt ggaggagccc gggcgcggca
     4981 gccggctcac tccaacatcc ttgcgccacg ccctctgccg caccgtgctg cgtt
//
LOCUS       DASREK010000303         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2291924, whole genome shotgun sequence.
ACCESSION   DASREK010000303 DASREK010000000
VERSION     DASREK010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2291924"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1357)
                     /gene="mnmA"
                     /locus_tag="VFA54_16405"
     CDS             complement(248..1357)
                     /gene="mnmA"
                     /locus_tag="VFA54_16405"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZS52454.1"
                     /translation="MSSEMIAVAMSGGVDSSTAAALLHRANEPILGLTMQLWNQRRLP
                     QLHAEGASSGRCCSLDDVYDARHVAEHLGIPYYVVNLERHFEERVVRPFVQEYLSGRT
                     PIPCTLCNNFIKFDRFLELARQVGASRIATGHYARVEWNPERRRYVLRKGLDDGKDQS
                     YFLFGLTQEQLARTLFPLGGMSKAEVRRMAAEMDLPVAYKSESQEICFVPNGDYAQFV
                     ESYMEERSEPLVPGAGKIVTAGGEILGEHSGIHHFTVGQRRGLRVAAGSPLYVISIDP
                     ETRQIVVGPEQQLLRRSFFAKDVNWIGLERLTGPIHVQVKIRHRFTPAAATVRPTGDP
                     SRVEVEFHTPQRSVTPGQASVFYNGDEVLGGGWIE"
     gene            complement(1363..2526)
                     /locus_tag="VFA54_16410"
     CDS             complement(1363..2526)
                     /locus_tag="VFA54_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZS52455.1"
                     /translation="MERLYFDHNATTPVAAEVVEAMLPCFSQAYGNASSIHFFGQQAK
                     LLLERARRQTASLLGCRPAEIVFTSGGTEANNLAILGVVRNSSRTRRHVITTAIEHPC
                     VLDACRQLEREGVEVTRVRVGSDGVVSPDDIRAALKPHTVLVSVMHANNEIGTVQPIQ
                     EISAAAHSAGVLMHSDGVQSAGKIPVDVRELGVDFYAFSGHKIYGPKGAGALYVRKTA
                     RLQSILFGGHQERGRRPGTENVAGAVGLGAAAELARRELLADAARLERLRDRLERAVL
                     ERIPFTGVNGAKARRVPNTTNIYFDCLEGEAMVIALDLKGMAVSTGSACSSGSVEPSH
                     VLSAIGLDAARARASIRFSLGRQNTDEQVDALVEAVEAAAAHLRKLSPAYPAA"
     gene            2621..>3044
                     /locus_tag="VFA54_16415"
     CDS             2621..>3044
                     /locus_tag="VFA54_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HZS52456.1"
                     /translation="MTTDFGLSDHYVGVMKAVILSLNAEAQIVDLCHQIEPYDLLGAA
                     LCLGASYTYFPAKTVHLVVVDPGVGSSRRAIVAEAGSWLFVAPDNGVLELIYQRHPHK
                     VWALRTEEFALRPTSQTFHGRDIFAPAAALLSKNTQPSQ"
BASE COUNT          607 a         1002 c          875 g          560 t
ORIGIN      
        1 gaaccccccg ccccggcctc atggagtgca ttgatgccgc caggaaagct tacgctgagg
       61 cgggcgcctc cgatcggttt gaggtaataa tcgaagaaaa tactggccac cgggtgactc
      121 ccgaggccag ggaacaggcc atccggtggt ttgtgagatg gctcaaaccg tagacccgac
      181 cgagcggaaa gacgttccaa cccactcacg cggatgtgct ttcagcgctc agagcaaggt
      241 cgctcgctta ctcgatccag ccgccgccca gcacctcgtc accgttataa aagacgctgg
      301 cctggccggg ggtgacggaa cgttggggag tatggaactc aacctccacc cgggacggat
      361 ctcccgtcgg ccgcacggta gccgccgcgg gagtgaagcg gtgccggatc tttacctgca
      421 catggatagg cccggtgagc cgctcgagac caatccaatt cacgtctttt gcaaagaagc
      481 tgcgccgcag caactgctgc tcgggtccca ctacgatctg ccgcgtttcc gggtcaattg
      541 aaatcacata gagaggcgac ccggccgcta cccgcagccc gcgccgctgg cccaccgtga
      601 agtggtgaat accggagtgc tcgccgagga tctctccccc cgccgtgacg atcttgccgg
      661 cgcccggaac cagcggctca gagcgctctt ccatatacga ctcgacaaac tgcgcgtaat
      721 cgccgttcgg cacgaagcag atttcctggc tctcgctctt ataggcgacc ggcagatcca
      781 tctcggccgc catccgccga acctccgctt tgctcatccc gccgagcgga aacagggttc
      841 gggcaagctg ctcctgggtc aggccgaaca aaaaatacga ctgatctttg ccgtcatcca
      901 agcccttccg caacacatat cttctcctct ccggattcca ctcgacgcgg gcgtaatgtc
      961 cggtagcgat gcgggacgcg cccacttgcc gggccagttc caggaagcga tcgaacttga
     1021 tgaagttgtt gcacagcgtg cagggaatcg gcgttcgccc cgataaatat tcctgcacaa
     1081 acggccgcac aacccgctcc tcgaaatgcc gttccaaatt cacgacataa tacggaatgc
     1141 cgaggtgctc ggccacgtga cgcgcatcat agacatcgtc gagagaacag catcgcccgc
     1201 tggaggcccc ttctgcatgc aactgcggca gccggcgctg attccagagc tgcatcgtga
     1261 gccccaaaat cggttcgttg gcgcgatgca gcagcgcggc ggcggtcgag ctatccacgc
     1321 cgccgctcat cgcgacggca atcatctcgg aagacattgg gtttacgcgg ccggataagc
     1381 gggcgagagc ttccggagat gcgcggcggc cgcctccacc gcctccacca gcgcatctac
     1441 ctgctcgtcg gtattctggc ggcctaaaga gaagcgaatg ctggcgcgcg cccgcgccgc
     1501 gtccaggcca atcgcactga gcacgtgcga cggctcgacc gagccgctcg aacacgccga
     1561 accggtagaa acggccatgc ccttcaaatc gagagcgatg accatggcct cgccctccag
     1621 gcaatcaaag tagatgttcg tggtgttcgg cacgcgcctc gccttggcgc cgttaactcc
     1681 cgtgaaagga atgcgctcga gtacggcccg ctccaggcgg tcgcgcagcc gttccagccg
     1741 agccgcatcc gcaagcaatt cccggcgcgc aagttccgcg gccgcgccaa ggcctaccgc
     1801 gccggcgacg ttttcggtgc ccggccgccg accgcgctcc tgatggcctc caaagagaat
     1861 cgattgcagc cgggcggtct tgcgaacata aagcgccccg gcgcccttgg ggccatagat
     1921 cttatggccg ctgaaggcat agaaatcgac tcccagttct cttacatcga ccggaatctt
     1981 gccggccgat tgcacgccgt cgctgtgcat caacactcct gcgctgtggg cggcggccga
     2041 gatctcctga atgggctgca ccgtgccgat ctcgttgttg gcgtgcatta cgcttaccag
     2101 cactgtgtgc ggcttcagcg cagcacggat gtcgtccgga gagacgacgc catcggaccc
     2161 cacgcgcacg cgcgtcactt caacgccctc tcgctccaac tggcggcagg catccagaac
     2221 gcaggggtgt tcaatggcgg tcgtaataac gtgccgccgc gtccggctgg agtttcgcac
     2281 cacccccaga atggccaggt tgttcgcctc ggttccgccg cttgtaaaca caatctcggc
     2341 cggccggcaa ccgagcagcg aggccgtctg gcgtcgagcc cgctccaaca gcaatttggc
     2401 ctgctgaccg aagaaatgaa tgctcgacgc gtttccgtag gcctgggaga agcatggcag
     2461 catcgcctcg acaacttcgg ccgccaccgg agtcgtcgcg ttatgatcaa aataaaggcg
     2521 ctccatgcga acctataggc gtattatagc aatcgtcgaa tccaacccgt tcgtcgggtc
     2581 ttgcaggtaa cagccgcaca tgtcccggga aattatcagt ttgactacag atttcggcct
     2641 ctctgaccat tacgtaggcg tcatgaaggc agtgattctg agcctcaatg ccgaagctca
     2701 aatcgtagac ctctgtcatc agatcgagcc ttacgatctg ctcggggcgg ctttatgcct
     2761 cggcgcctcg tacacttatt tcccggcaaa gaccgttcat ctggtcgtcg tcgatccggg
     2821 tgttggcagt tcgcgccggg cgatcgtcgc cgaggcgggg tcgtggctgt ttgtagcgcc
     2881 cgataacggc gtcctcgagc tgatatacca acggcaccca cacaaggtat gggcgctccg
     2941 caccgaggaa ttcgctctcc ggcctacgag tcaaacgttc catggaaggg acattttcgc
     3001 ccccgcggcc gcgctgctct caaaaaatac gcagcccagc cagc
//
LOCUS       DASREK010000304         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_817301, whole genome shotgun sequence.
ACCESSION   DASREK010000304 DASREK010000000
VERSION     DASREK010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817301"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFA54_16420"
     CDS             <1..345
                     /locus_tag="VFA54_16420"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZS52457.1"
                     /translation="VLLSLEDAAAIVKQIGSPGIQTIYDPHNAVDETEPPEVLVDRYF
                     EIIRHVHLNELDGTYPRPGGGYDFQPVLQVLKNRGYAGWLSMEIFDFSPGAEKIITEA
                     GAYLRDEISKLA"
     gene            455..1390
                     /locus_tag="VFA54_16425"
     CDS             455..1390
                     /locus_tag="VFA54_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS52458.1"
                     /translation="MRIVVTGGAGFIGSALVRGLIAQNLGSSILVADNLLTGKRSNLL
                     DVEDRIEFQQADIRDYPAVEGLLRKADLLFHLAAVPSVPRSISDPVLAHEVNIDGTFN
                     VLLAAQRCGVRRVVYAASSSAYGDTPTLPKIETMLPMPKSPYAVQKLLGEYYGRVFTE
                     VYGLETVSLRFFNVFGPRQDPSSPYSGVISVFISALLDRRAPTIFGDGEQTRDFTYVD
                     NIVQLCIKAAQAPEAAGKLYNGGVGARITLNETWRLLQKIEGVEIPPVYGPPRPGDVR
                     DSQADISAARRDLSYQPCVDFETGLRRTLEWFRSK"
     gene            1580..2983
                     /locus_tag="VFA54_16430"
     CDS             1580..2983
                     /locus_tag="VFA54_16430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZS52459.1"
                     /translation="MQQEGRKEKTPSALTLLVASEASPECTISESLVAGRFLVHSVPV
                     AQVESWLAQSDADAVVVYSNRGAQELTRLVKIWKSIQPRAQFILLLARAPSARELVAL
                     MHAGYQDVLEQAAADMSSVLQSLEERVRYVRVRQMERLQVKQSIQYAGLIGESREMVR
                     IYDEIVRTARLNCPVLLLGETGTGKGLVAHAIHALSHRSGRAFVTVDCACLAPTLIES
                     ELYGVARGAYTGATADRSGLVEAAQHGTLFLDEVGELPLGMQPKLLRLLEEREVRRLG
                     SPRSTPVDVRIISATSRSLEALIAADQFRLDLYYRLNVLAIEIPPLRQRLQDVPLLAR
                     HFASRHLVHGRPAEISGAAVAVLLEYSWPGNVRELKNCIEAALARAGGNVIQVQHLPS
                     WFGRGAEAGGSEQERLNLKQIERRTILRALDLAANDKRKAARWLGIGKTTLYRKLKQM
                     TGSSRTASAPAKPGYVM"
     gene            3137..3625
                     /locus_tag="VFA54_16435"
     CDS             3137..3625
                     /locus_tag="VFA54_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52460.1"
                     /translation="MPFCPSCGAKVEGSFCGSCGARVGDVPPAAMPAQAGASPSAAVP
                     AVAGLQDNVAGALCYLLGIITGVLFLVLEPYNRNRQIRFHAFQSIFLHVALIAVWIAL
                     AMLGILMRGIFSIFGLLLIPVHFIVGIGAFILWIVLLVKTFNGSKLVLPVIGPLAEKQ
                     AG"
     gene            3745..>4411
                     /locus_tag="VFA54_16440"
     CDS             3745..>4411
                     /locus_tag="VFA54_16440"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HZS52461.1"
                     /translation="MIHITESQVRELLPMRKALELVETGFLHLAEGKAKNHPRRRLQL
                     GSSATLHYMAAVDTASGYLGTKLYTTHPQTGAHFTVVLYAADGGPLASIEANALGQIR
                     TGAASGVATRLLARPDAAVLGLIGSGFQAETQLEAIALVRSLKEVRVYSRSVENRRRF
                     AEKMSRWLRLAIEPVETAEKAIRGCDIAVTATNARDPVLLGEWLEPGMHINAIGSNHF
                     LRRE"
BASE COUNT          791 a         1340 c         1380 g          900 t
ORIGIN      
        1 gttctgctga gcctggagga tgccgccgcc atcgtgaaac aaattggcag tcccggcatc
       61 cagactattt atgatcctca caatgcggtc gatgagacgg agccccctga agtgctagtg
      121 gaccgctact tcgaaatcat ccggcacgtg catctaaacg aattggacgg cacctatccg
      181 cgtccgggcg gcgggtacga cttccagcct gttcttcagg tgttgaaaaa tcggggctac
      241 gcgggctggc tctcgatgga aatcttcgat ttctcgcccg gggcggagaa gatcattaca
      301 gaagccggcg cctacctgcg cgacgagatc agcaagctgg cctagcggga ggaccgctcc
      361 gggcgcgctc cactattttc gcgggaaagg ctggccgttg tggcgcgtaa tttgctatcc
      421 gaaacggcgt tttgcgcaaa acggcttccg agttatgcga attgtcgtga caggcggcgc
      481 aggttttatc ggctcggccc tggtgcgggg actgatcgcg cagaatctgg gaagcagcat
      541 tctcgtcgcg gacaacctgc tgacgggcaa acggagcaat ctgctcgatg tggaggaccg
      601 gatcgaattc cagcaggccg acatccgcga ttatccggct gtcgagggac tgcttcgcaa
      661 ggccgatctg ctgttccact tggcggcggt cccctcggtg ccgcgctcga tttccgatcc
      721 ggtattggcg catgaggtca acatcgatgg cacgttcaat gtcttactgg cggcccagcg
      781 gtgcggcgtc cggcgcgttg tttatgccgc ttcttcttcc gcatatggcg acacgcctac
      841 tctgccgaag attgagacca tgcttcccat gcccaaatcg ccctacgccg tgcagaaact
      901 ccttggcgag tactatgggc gggtgtttac cgaggtgtat ggtctcgaga cggtttcgct
      961 gcgctttttc aacgtgtttg gtccgcgcca ggacccttct tcgccctact ccggcgtaat
     1021 ctccgtcttt atttcagcgc ttcttgaccg gcgcgcacct acaattttcg gcgacggcga
     1081 gcagacccgc gacttcacct atgtcgataa catcgtccag ctttgcatca aagccgccca
     1141 agcccctgaa gccgccggca agctctacaa cgggggcgtg ggcgcgcgca tcacgctgaa
     1201 cgaaacctgg cggctgctac agaagattga gggtgtcgaa attccgcctg tttatggccc
     1261 gccccggccc ggcgatgtcc gcgactccca ggcagatatt tctgccgcgc gccgcgacct
     1321 gagctaccag ccctgcgttg atttcgagac cgggctgcgc cgcacgctcg agtggtttcg
     1381 ttccaaatag ctcagatccg cccctcgccc gactgttgcc ctttgcttgc tccgccgatt
     1441 ttagtttggg aatggctccg gacggcccgt gacgcgaagg accggcgcga aagcgcgcgc
     1501 tggcttcatt cccgcggctt cgttattgca atcgcagggt cccccaaagc aagggccgcc
     1561 atcggtttcg gagcgggcta tgcaacaaga agggcgaaaa gaaaaaacgc ccagcgcgct
     1621 taccctgctt gtcgcgagcg aagcctcgcc tgagtgcacg atctctgagt cgctggttgc
     1681 cgggcgattt ctcgttcatt ccgttcctgt tgcacaggtg gagtcatggc tggcgcagtc
     1741 ggatgcggac gccgtcgttg tgtacagcaa tcgcggcgcc caggaactga cccgcctggt
     1801 caagatctgg aagtccattc agccacgggc ccagttcatt ctcttgctgg caagggcccc
     1861 ctcggcgcgg gaactggtcg cgctaatgca cgcgggctat caggatgttt tggagcaggc
     1921 ggcagccgat atgagcagcg tgctccaatc cctcgaagag cgcgtccgct atgtgcgggt
     1981 gcgccaaatg gaacggctcc aggtcaagca gagcatccag tatgcggggc tgatcggcga
     2041 atcgcgggag atggtgcgga tctatgacga aatagttcgt acggctcgcc tgaactgccc
     2101 ggtgctgctg ctgggggaaa cggggacagg caaggggctg gtggcgcacg cgattcacgc
     2161 cctcagccat cgcagtggca gggcttttgt aaccgtcgat tgcgcgtgcc tcgctcctac
     2221 gctgattgaa agcgagctat atggcgtagc ccggggagcg tacaccggcg ccaccgccga
     2281 ccgctccggc ctggtggagg ccgcgcagca tggcacgctg tttttggatg aggtgggtga
     2341 gttgccgctt ggcatgcagc ctaagctttt gcggctcctc gaagagcgcg aggtccgccg
     2401 gctgggcagc ccccgcagca ccccggtgga tgtccgcatc atctcggcga ccagccggag
     2461 cctcgaggct ttgattgccg ccgaccagtt tcgcctcgac ctctattacc gcctgaacgt
     2521 cctcgccata gaaattccac cgttgcgcca gcggttgcag gatgttccgc tgctggcgcg
     2581 tcacttcgcc tcccggcatc tcgtccatgg ccggccggcg gaaatctccg gcgctgcggt
     2641 cgccgtgctg cttgagtact cctggccggg caacgtgcgg gagctgaaaa actgcatcga
     2701 ggcggccctg gcccgggcgg gcggcaacgt aattcaggtg caacaccttc cttcctggtt
     2761 cgggcgcggc gccgaggccg gcggttccga acaagaacgg ctcaatctga agcagatcga
     2821 gcggcgcact atcctgcggg cgctggatct cgctgcgaac gacaaacgca aggcggcacg
     2881 ctggctcggg atcggcaaaa ccaccctcta ccgcaaactg aagcaaatga ccggatcatc
     2941 caggactgcc tccgcccctg ccaagcctgg ttacgtgatg tagcgcggtg cgcagcgctg
     3001 cgaacaggct ttcccggaca cctgcgcagc atccggtatt ccggcccgga aaatagcagt
     3061 tgcaagtact tgcgactagg cgtataaaca acacgtttac acgccgaccg aatggctggc
     3121 caaggaggta agtaatatgc cgttctgccc gagttgcggc gccaaggtag aaggcagctt
     3181 ttgcggctct tgtggagccc gggtagggga cgttccgccg gctgcaatgc cggcgcaggc
     3241 gggggcgagc ccgagcgcgg ctgttcccgc cgtggcgggc ctgcaagata atgtcgccgg
     3301 cgccctctgc tatctgctgg ggatcattac cggcgtgctt tttctcgtgc tcgaacccta
     3361 caaccgcaac cgccaaatcc ggttccacgc cttccaatcc atctttctgc atgtggcgct
     3421 gattgccgtg tggatagcgc tcgcgatgct gggcattctg atgcgcggga ttttttctat
     3481 ttttgggctg ctgctgatcc cggttcattt tattgtcggt atcggcgcgt tcatcctatg
     3541 gattgtgcta ctggtaaaga cctttaacgg cagcaaactc gtcctgcctg tcattggccc
     3601 cctggctgaa aaacaggccg ggtgagcgag ccttgcctgt ggcctgcccg tctcgtggag
     3661 agggcggggg ggcgctcctt tgcgccagca gggcggctca gcgcctgcac ttcttcgatg
     3721 caggccggct gatatccttt tgccgtgatc cacatcaccg agtcgcaggt tcgcgagctc
     3781 ctgcccatgc ggaaggccct cgagctggtg gaaaccggat ttctccatct ggcggaaggg
     3841 aaggcgaaaa accacccgag acggaggctc caactgggat caagcgccac tttgcattac
     3901 atggcggcgg tcgataccgc ttctgggtat ctcggaacaa aactgtacac cacgcatccg
     3961 cagacgggag cccatttcac ggtggtttta tatgcggccg acggtggtcc cctggcgtcg
     4021 atcgaggcca atgccctcgg gcagattcga accggggcag cgagcggcgt ggccacgcgg
     4081 cttctggcgc gtccggacgc tgccgtgctg ggcctgatcg ggagcggttt tcaggccgaa
     4141 acccagcttg aggcgatcgc gctcgtgcgc agcttgaagg aagtgcgtgt ctacagccgg
     4201 tcggtggaaa accgccggcg tttcgccgaa aagatgagcc gttggttgcg attggccatc
     4261 gagcccgtgg aaacagcgga gaaagcgatc cggggatgcg atattgcggt caccgccacc
     4321 aacgcgcggg accccgttct tctaggcgag tggctcgagc cgggcatgca catcaacgcg
     4381 atcggcagca atcactttct gcggcgtgag c
//
LOCUS       DASREK010000305         5994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_147479, whole genome shotgun sequence.
ACCESSION   DASREK010000305 DASREK010000000
VERSION     DASREK010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147479"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFA54_16445"
     CDS             complement(<1..959)
                     /locus_tag="VFA54_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HZS52462.1"
                     /translation="MGIQRVDSFSRRRFLGLAATAPFLARFEQLAAAERKRVKIRDVQ
                     TLILKGPRTYTLVKVISDSGLYGIGEAYGSPGVGVKEQVLSLKPWLVGKDPLEIDALY
                     TFMGEHTPSLSGTRTDGSAHMLLRAVSGIEMALWDLAGKILDVPATTLLGGKFRDKVR
                     VYDHAAPRNMLDKASCREWAQKVKADPRGFTCHKFGFPNSDPSTDKARDPANRVLTTK
                     ELMQIRQGFENCREAVGWDHDIMVHCHWEYDLRTAIQIAEAVESIKPVWLEDPMGVDY
                     SESWKRLCASSRVPICTGENLARREGFKDFIINQGCDILHADLR"
     gene            complement(1010..1141)
                     /locus_tag="VFA54_16450"
     CDS             complement(1010..1141)
                     /locus_tag="VFA54_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52463.1"
                     /translation="MKFSVAVLRRVGASAAYKPSPRAPRRWDVRPGLTVERGRNSVV"
     gene            1262..2629
                     /locus_tag="VFA54_16455"
     CDS             1262..2629
                     /locus_tag="VFA54_16455"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS52464.1"
                     /translation="MSLTSRRHFVKSAALAGAAVRAAWPQRSPNEQINLGIVGIHGRG
                     REHYNFFSKAPNVRVAFLCDIDERLFPQGVAEVEKLYGRKPETFVDIRKMLERKDLDA
                     ITVATPDHWHALHTIWACQAGKDVYVEKPVCYCLREGRKMVEAARKYNRIVQAGLNRR
                     CEPRIRAGAKFVREEKFGKVYRAKAIVYRGRLPIGKVPESSIPEGVNWDMFLGPAPYK
                     PFSLNRFHYGWHFFWDTSTTEVGNNGVHYLDQIRLAWGDKNEHPVKITCTGGLFLNES
                     DQEVPNYQVASFEYADGSIIDLEVTNLPTPSIGGILEGVFFYTRQGYVTSANNWTTVV
                     GEFTARDTPDTPTGVSNRVNRLSFPRVAYKPGPPIPDLNEPEVTHWQNFIDCVRSRRR
                     EDLRCEILEGHMSTALCHLANIAFRTGRKLTFDPATETFPGDAEANRLLTRAYRAPYV
                     VPDQV"
     gene            complement(2664..3824)
                     /locus_tag="VFA54_16460"
     CDS             complement(2664..3824)
                     /locus_tag="VFA54_16460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS52465.1"
                     /translation="MHTHHNRDRHDCSRRGFLGRLLASTLPGASVLELSIARAACARA
                     QAAGANPQLFDIGKITSGVYAAIARPAVLTNCNAVIFERSADLLVVDTHSKPSAVASL
                     VAQIRREVSPKPVRYVVNTHFHYDHTQGNPEYQKQFSKLDIVASETTKKLMVELAEKR
                     LKASLDAIPASIAQAEEKAAKTRSAEERAFFEDEARQYRAYLAEMKDYRVALPTITFA
                     ESYVIKDKAGDIHLSFHGRAHTASDIVVFSPVKKVLAAGDLIHGSFPTITDGYPREWP
                     ATLDAVAKIGFTYAAPGHGPVQKGAARIRNMRNYIEELAARVADGKRAGKSVAELQKT
                     ITVSSLRSLAADGYADYILANRSAGYRRLGPIRMQDGIDGNIAQIYEALEHR"
     gene            complement(3845..4615)
                     /locus_tag="VFA54_16465"
     CDS             complement(3845..4615)
                     /locus_tag="VFA54_16465"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZS52466.1"
                     /translation="MRRLLAALVLLAPLTAQVTSQSKPSTGVAPDPVYISANRPRALA
                     LIGDIYHGPVMMRDGLITALVKENIPVTFIEDPAALNAKSLAEFSLLIIARNGRYWPQ
                     GYGKPQTFWMTDEQQKAIWDFVQNGGGFLALHNAHTMYPPDGLYYKLFGGNFGGHPKP
                     WTFTIRVENKNHPITAGVEDFDIFDEQHLSKYYLDQEHLLLRSVARDNQQAAAGWWRE
                     MGKGRFCYLSPGHTPEALNHPMMQRLIRNASRWLLHMN"
     gene            complement(4854..4927)
                     /locus_tag="VFA54_16470"
     tRNA            complement(4854..4927)
                     /locus_tag="VFA54_16470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4893..4895),aa:Gln,seq:ttg)
     gene            4995..5660
                     /locus_tag="VFA54_16475"
     CDS             4995..5660
                     /locus_tag="VFA54_16475"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HZS52467.1"
                     /translation="MPVNHDLRAAAASENGRPDRAQPLHAALLAGLLGVFVWSGIHPH
                     DYFTWLLEVGPAVIGIGILAATYSRFRFTTLVYVLVCVHAVILMIGGHYTYAEVPLGY
                     WMERAFHMTRNNYDKIGHFAQGFAPALVAREVLLRTSPLRRGGWLFFIVVSICLAISA
                     AYELLEWAVAVLSGTAADAFLGTQGYVWDTQSDMLFALIGALAALLLLGQAQDKQLGK
                     LGL"
     gene            complement(5675..>5994)
                     /locus_tag="VFA54_16480"
     CDS             complement(5675..>5994)
                     /locus_tag="VFA54_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52468.1"
                     /translation="AGHPRGEDLRGAVCPLTTYGGGNMRLGPSFWRKRVWGAPASAPF
                     SYCAGRARKAGALLLALLRELGDENAYQRHLAARGCPASAEEWRRFSAARFAARFERP
                     KCC"
BASE COUNT         1158 a         1838 c         1809 g         1189 t
ORIGIN      
        1 cgcagatcgg cgtgcagaat gtcgcatccc tgattgatga tgaagtcctt aaagccctcc
       61 cgccgggcca ggttctctcc ggtgcaaatc ggaacgcggg aactggcgca caagcgtttc
      121 caggattcgg agtaatccac ccccatcggg tcctccagcc aaaccggttt gattgactcg
      181 acggcttcgg cgatctggat cgctgtccgc aggtcgtact cccaatggca gtggaccatg
      241 atgtcgtgat cccagccgac cgcttcccgg cagttctcga atccctgcct tatctgcatc
      301 agctctttcg tcgtaagaac ccggttggcc gggtcgcggg ccttgtccgt actcggatcc
      361 gaatttggga agccgaactt gtggcacgtg aagccccgcg gatcggcctt taccttctgg
      421 gcccactcgc gacaggacgc cttatccagc atgttgcggg gagcggcgtg atcgtacacg
      481 cgcactttgt cgcggaattt tcctccgagc agcgttgtag ccggcacgtc cagaattttg
      541 ccggccagat cccacagcgc catctcgata ccgctcaccg cccgcaacag catgtgagcc
      601 gagccgtcag tgcgcgtgcc gctgagcgag ggcgtgtgct cgcccataaa ggtataaagc
      661 gcgtcaattt cgagcgggtc tttgcccaca agccagggct tcagagagag cacctgttct
      721 tttacgccga cgccggggct tccgtaggcc tcgccgattc catacaagcc ggagtcagag
      781 atgaccttta ccagtgtgta agtgcgcggc cctttcaaaa tcagggtctg cacatcgcgt
      841 atcttgacgc gcttcctttc agccgcggcg agctgctcaa agcgggccaa aaacggagcc
      901 gttgcagcca gaccgagaaa gcggcggcgt gagaaagaat cgacacgctg tatgcccata
      961 aatacctcca ggaacgctgt ggcgcccggg ccggatttgt catggcacat catacaaccg
     1021 aattgcgccc gcgttccaca gtcaacccgg gccgcacgtc ccaccggcgg ggagctcgcg
     1081 gcgacggttt gtaagccgcc gaagcgccta cccggcgcag cacggcaacc gaaaatttca
     1141 ttggggggaa cgcttttccc ccgtgagaat ggatggactc cggcgctgaa atcgcggata
     1201 atccaagcac aagaatagaa gaggcgctcc gccgcctgga gccgccggta aggaggtcat
     1261 catgtcactg acgagccgtc gccatttcgt gaaaagcgct gcgctagcgg gtgctgccgt
     1321 gcgcgccgcc tggccgcagc gcagccccaa cgagcaaatc aacctgggaa tcgtcggcat
     1381 acacggccga ggccgcgagc attataactt tttctcgaag gcgcccaacg tccgtgtggc
     1441 gtttctctgc gacatcgacg agcggttgtt tccgcaaggc gtcgctgagg tggagaagct
     1501 ctatggccgc aagccggaaa ccttcgtgga catccggaag atgctcgagc gcaaagatct
     1561 ggacgctatc accgtggcca cgcccgatca ttggcacgcg ctccacacga tctgggcctg
     1621 ccaggcgggc aaagacgttt atgttgaaaa gcctgtctgc tactgtctgc gagagggccg
     1681 gaaaatggtc gaagcggcgc gcaaatacaa ccggatcgtg caggccgggt tgaaccggcg
     1741 atgcgagccg cgcatccgcg ccggcgcaaa attcgtgcgg gaggagaaat tcggcaaggt
     1801 ctaccgcgcg aaggccatcg tttaccgcgg ccggcttccc atcggcaaag ttccggagtc
     1861 gtcaatcccc gagggcgtga actgggacat gtttctcggc cctgcgcctt acaagccctt
     1921 ctcgttgaac cgattccact acggctggca cttcttttgg gatacatcca cgaccgaagt
     1981 cggcaacaac ggcgtacatt atctcgacca gatccgtctc gcctggggcg ataaaaacga
     2041 gcatccggtg aaaatcacgt gtacgggcgg gcttttcctg aacgaatcgg accaggaggt
     2101 gcccaattat caagtggcgt cgttcgagta cgccgacggg tcaatcatcg atctcgaggt
     2161 caccaatctg ccgacgccct cgattggggg aattctcgaa ggcgtgttct tctatacccg
     2221 gcaaggttat gtcacctcgg ctaataactg gactaccgtg gttggggaat tcaccgcccg
     2281 agacacgcct gatacgccca caggcgtttc caaccgtgtc aaccgcctca gcttcccacg
     2341 ggttgcttac aagccggggc cgccgatacc cgacttgaac gagccggaag taacccactg
     2401 gcagaatttc atcgactgtg tgcgatcgcg aaggcgggaa gatctgcgct gcgaaatcct
     2461 ggaggggcac atgtccactg cgctgtgcca tctggcgaac atcgcgttcc ggacgggcag
     2521 gaagcttacg ttcgatccgg cgaccgaaac gttccccgga gacgccgagg cgaaccggct
     2581 gctcacgcgc gcttaccgcg cgccttacgt ggtgccggac caggtatagc ttcgccggaa
     2641 ttcggaacgc tcggccgtgc ggcctaccgg tgctccaggg cctcgtaaat ctgagcgata
     2701 ttgccgtcta tgccgtcctg catgcggatc gggccgaggc gccggtagcc cgcggaccgg
     2761 ttggcgagga tgtaatccgc gtagccgtca gccgccagag atcgcagcga gctgaccgtg
     2821 atcgtttttt gcagttctgc gacggatttc cccgccctct tgccatccgc cacgcgcgcg
     2881 gcgagctcct cgatgtagtt ccgcatattg cgaattctcg cagcgccctt ctggacgggg
     2941 ccgtggccgg gcgccgcgta ggtaaagccg attttcgcga ctgcgtcgag cgtggctggc
     3001 cactcgcgcg gatagccatc cgtgatggtc gggaatgagc cgtggatcag atcgcccgcg
     3061 gcgagcacct ttttcaccgg tgaaaaaaca acaatatcgc tggcggtatg cgcccggccg
     3121 tgaaagctca ggtgaatatc gcctgccttg tccttgatca cataggactc ggcgaaggtg
     3181 atggtgggga gagcgacacg gtaatccttc atctccgcca gatacgcccg atattgccgg
     3241 gcttcgtcct cgaagaacgc cctctcctcc gcgcttctcg ttttggccgc cttctcctcc
     3301 gcttgcgcga tggaggccgg gatcgcgtcg agcgaagcct tgagccgttt ttccgccagt
     3361 tccaccatga gcttcttggt ggtctcgctg gccacgatgt ccagcttcga gaattgcttc
     3421 tggtattccg gattgccctg cgtgtgatcg taatggaaat gcgtgttcac aacgtagcgc
     3481 accggcttgg gagaaacctc ccggcggatc tgagccacga gcgaggcgac tgccgatggc
     3541 ttggagtgcg tatcgaccac cagcagatcc gctgaccgct cgaagatgac cgcgttacag
     3601 ttggtcaaaa ccgccggacg cgcgatggcg gcgtaaacgc cgctggtgat tttcccgatg
     3661 tcaaaaagtt gcgggttggc ccccgccgcc tgcgcacggg cgcaggcggc gcgggcaatg
     3721 ctcaattcga gcaccgaagc gccgggcagc gtagaggcga gcagccggcc caaaaagccg
     3781 cgccgggagc agtcatgacg atcgcgattg tggtgcgtgt gcatctcatc tcctgtgttt
     3841 gcgtctagtt catatgtagc agccaccgcg acgcattgcg aatgagccgc tgcatcatcg
     3901 gatgattcaa tgcttccggc gtatgacccg gcgaaagata gcaaaaccgc cctttcccca
     3961 tctcgcgcca ccagccggcg gcagcctgct ggttatcgcg cgccaccgaa cgcagcagca
     4021 ggtgctcctg atccagatag tatttcgaga ggtgctgctc gtcgaaaata tcgaaatcct
     4081 ccactccggc ggtgatggga tggttcttgt tttccacgcg tatggtgaac gtccacggct
     4141 tcgggtggcc tccgaaattg ccgccgaaca gcttgtaata aaggccatcc ggcggataca
     4201 tcgtatgcgc gttgtgcaag gcaaggaaac cgccgccgtt ctggacaaag tcccagatgg
     4261 ccttttgctg ctcgtcggtc atccaaaatg tttgcggctt gccgtatccc tgcggccagt
     4321 atcgcccgtt gcgggcgatg atgagcagcg aaaactccgc cagcgatttg gcattcaaag
     4381 cggcagggtc ttcaataaaa gtaacgggga tattttcttt tacaagagcg gtgatcagcc
     4441 catcccgcat catgacggga ccgtggtaaa tgtcgccgat cagagcaaga gcgcgggggc
     4501 gattggcgga gatgtagacg gggtccggcg ccactcccgt gctcggcttt gactgcgatg
     4561 tgacttgcgc cgtcaaaggc gcgagaagga ccaaagccgc cagcagcctt cgcatgatgc
     4621 ctcatcttaa aacagccata gcgcctgggc aatgcaagca gcctgacccg atcgcagttt
     4681 ggcctaaggg ttcggatcag cgtgcgccta cgggaagaac ctgtggatgc acaacgggcc
     4741 ggcttgaacc aacgctctgg ctgaccggcc ggaaaagcct ttcgccagct tgggattcgg
     4801 gtccgtccct ttttgcaagc tcagaggcct aagggaattg ccttgcttcg caatggctga
     4861 ccggggagga ttcgaacctc cattccagga tccaaagtcc cgtgtcctgc cattggacga
     4921 ccggtcaagg aacgcggccc ggcgctaggc gggcaaacaa agtcagtgta acatgagctg
     4981 cgcgggatgt ttgcatgcca gtaaaccatg acctccgcgc cgcagcggct tcggagaatg
     5041 gccgcccgga ccgggctcag cccctgcatg cagcgctgct cgccggtctc cttggtgttt
     5101 tcgtctggtc agggattcac ccgcacgatt atttcacctg gcttctggaa gtcgggccgg
     5161 ctgtcatcgg cattgggatc ttggccgcca catacagccg cttccggttc acgacactgg
     5221 tctacgtact ggtgtgcgtg cacgccgtca tcctgatgat cggcggccat tacacgtatg
     5281 cggaagtccc gctcggttat tggatggagc gggcctttca catgacccgg aacaattacg
     5341 acaagattgg acacttcgcg caaggctttg cgccggcgct tgtcgcccgc gaagtcctgt
     5401 tgaggacttc tcccttgcgc cgcggcggct ggctgttttt catcgtggtt tcgatttgtc
     5461 tggccatcag cgccgcctac gagcttcttg agtgggccgt agccgtactc tccggaaccg
     5521 ccgccgacgc attcctcggg acgcaaggct acgtctggga tacgcagtcc gacatgctgt
     5581 tcgctctgat cggagcgctc gcagcgttat tgctgttggg ccaagcgcaa gacaaacagc
     5641 tcgggaaact cggcctttga cgcggcccgc cgcttcaaca gcatttgggg cgctcgaagc
     5701 gggccgcgaa gcgggcggcg gagaaacgcc gccactcctc ggcggaggct gggcagccgc
     5761 gagccgccag atgccgttgg taagcgttct cgtctcccag ctctcgcagc agcgccagca
     5821 gcagcgctcc ggctttccgg gcgcggcccg cgcagtagct gaaaggggcg gatgccggcg
     5881 ctccccacac tcgcttccgc caaaaagacg gcccgagcct catatttcct ccgccgtaag
     5941 ttgtgagggg acaaacggcg cctcgaaggt cctcgcctcg cgggtgcccc gcag
//
LOCUS       DASREK010000306         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_818062, whole genome shotgun sequence.
ACCESSION   DASREK010000306 DASREK010000000
VERSION     DASREK010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818062"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFA54_16485"
     CDS             <1..251
                     /locus_tag="VFA54_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52469.1"
                     /translation="LLAFIISFDDYLITSLVAGVGSETLPMVIYSMARRGISPVVNAL
                     SAILVVALGGLILIADRLQQGSEHWKVGTFESPAAPGK"
     gene            248..1339
                     /locus_tag="VFA54_16490"
     CDS             248..1339
                     /locus_tag="VFA54_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZS52470.1"
                     /translation="MKQAGQDSAEQICWSWSRNFSRRRLGAALAAAALAACARRRPEG
                     EVNVYCWSDYVAPDTVASFERETRLRVNYETFESQEEMLAKILAGGAGWDVVFPGNTF
                     IGPMVRRGLIEPIDHQRLPNLSNLNPRFTDPPWDRGIQHSVPYMWGLTGIVYNRRLIG
                     RTLSRWSDLWDPPLKGKITMLDDPAEVLGACLKKLGYSLNSVNEKELQAAKAEAIRQK
                     PLLRAFINAEVKPQLIAGDVWAAQLWNGDAYQAIAENKDLEYCLPEEGFALYTDSAAI
                     LTQSKHQDAAYQWINYLLRPDVAAGIARATMFPTPNARALGLLDAAVRQNPDLYPPPE
                     RLQKAEWFAEIAPEGQRLRDRIWTEIKAA"
     gene            1544..>2330
                     /locus_tag="VFA54_16495"
     CDS             1544..>2330
                     /locus_tag="VFA54_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52471.1"
                     /translation="MLQVIARGHDVFDAVVVGSGATGGWAAKQLTEAGMRVALLEAGK
                     KLTPAEFTEHLHPYDLKYRGYDPHKLERRPIQGLNYACRESNQQWFVDDIENPYTTPE
                     GKPFRWIRCRILGGRSLVWGRQSYRLGDINLKAASRDGYGDDWPISYADLVPYYEIVE
                     KFIGISGQPEGLEVLPDSVFLPPMEMTCGEMLLRDAVKKKMGRVITIGRVAIVTRPHN
                     GRQACHYCGPCEQGCITNSYYASPRITVAAAEKTGRLTLLTDSV"
BASE COUNT          447 a          723 c          725 g          435 t
ORIGIN      
        1 ccctgctggc ttttatcatt tcctttgacg actacctcat tacaagcctc gtggccggcg
       61 tcgggtccga gactctgccc atggtgattt attcgatggc gcgccggggc atcagcccag
      121 tggtcaatgc gctttcggcg attttggttg tcgcgctggg cggcttgatt cttatcgccg
      181 accggttgca acaaggctct gaacattgga aagtcgggac cttcgaaagt ccggcggcgc
      241 ccggaaaatg aaacaggccg gtcaggactc cgccgagcaa atctgctggt cctggagccg
      301 gaacttctcc cgccggcgtt tgggtgcagc gctcgcggcc gccgcgctcg ccgcctgcgc
      361 gcgccggcgc ccggaaggcg aggtcaacgt ctactgctgg tcggattacg tcgcgcccga
      421 cacggtggcg tctttcgaac gggaaacgcg gcttcgcgtg aactatgaga ccttcgagag
      481 ccaggaggaa atgctggcca aaatcctggc gggcggagct ggctgggacg tggtgtttcc
      541 aggcaacacg ttcatagggc cgatggtgcg gcgaggcctc atcgagccaa tcgaccatca
      601 gcgcctgccc aatctttcga atctgaatcc gcgcttcacc gatcctcctt gggaccgggg
      661 catccagcac agcgttcctt acatgtgggg tctcacgggc attgtataca accgccgatt
      721 gattggcagg acgctcagcc gctggagcga cctttgggac ccgcccctga aggggaagat
      781 cacgatgctc gacgacccgg ccgaggtgct gggcgcgtgc ctgaagaagc ttggctacag
      841 cttgaattct gtcaacgaga aggagctgca agcggcgaaa gccgaggcca tccgccagaa
      901 gcctctgctg cgggcattca tcaatgcgga ggtaaagccc caattgattg caggcgacgt
      961 ttgggccgcc cagctctgga atggcgacgc ctaccaggct atcgccgaaa acaaagatct
     1021 cgaatattgc ctgccggagg aggggttcgc tctttatacc gacagcgcgg cgatcctcac
     1081 gcaatcgaag caccaggatg cggcctacca gtggatcaac tatctgctgc gccccgatgt
     1141 ggcggccggc atcgcccggg ccacaatgtt tcccacgccg aacgcccgcg cgctcgggct
     1201 gctcgatgcg gcggtgcggc agaaccctga cctctatccg ccgccggaac gcttgcagaa
     1261 agcggagtgg ttcgccgaga ttgcgccgga aggccagcgc cttcgagacc gcatctggac
     1321 ggagattaag gccgcgtaaa gggaaggccg ctcaggccac ctgatgaaca gccgccgcag
     1381 cctgccgcga tggcgcccgg cccgcgccgc cagcctgccg ttgcagcttg cagtacgctt
     1441 cctggcccgg gagaatttca gagcgatctg caaccgcctt cacccgttcc ggcccttctt
     1501 ccagtggtcc gcaggcagct tcggtccttg ctacaatgca gccatgctac aggtaattgc
     1561 gcgcggccat gatgtttttg acgctgtggt cgtagggtcg ggcgccaccg gcggctgggc
     1621 tgccaagcag ctcaccgagg cgggcatgcg agtggcgctg ctggaagccg gcaaaaagct
     1681 cacgcccgcc gagttcaccg agcacctcca cccttacgac ctgaaatatc gcggctatga
     1741 tccgcacaag ctcgaacgcc ggccgattca gggcctcaat tacgcttgcc gggaatcgaa
     1801 tcagcaatgg tttgtggacg acatagagaa tccttacacg actcccgaag gcaaaccgtt
     1861 ccgctggatt cgctgccgca ttctgggcgg gcgctcgctg gtgtggggcc gccagagcta
     1921 tcggctgggc gacatcaatc tcaaggccgc cagccgggat ggctatggcg acgattggcc
     1981 gatttcctac gccgacctgg tcccgtatta cgagattgtc gagaaattca tcggcatcag
     2041 cgggcagccc gaggggctgg aagtgttgcc ggacagcgtt tttcttccgc ccatggaaat
     2101 gacgtgcgga gaaatgctcc tgcgcgatgc cgtcaaaaag aaaatgggcc gcgtcatcac
     2161 gatcgggcgc gtggcgatcg ttaccaggcc gcacaacgga cgccaggcgt gtcactactg
     2221 cggcccttgc gagcagggat gcatcacgaa ctcctattat gcaagtccgc gcatcactgt
     2281 cgccgccgcg gagaaaacgg gcagactcac cctgttgacc gattcggtcg
//
LOCUS       DASREK010000307        28199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1307401, whole genome shotgun sequence.
ACCESSION   DASREK010000307 DASREK010000000
VERSION     DASREK010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 28199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28199
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307401"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VFA54_16500"
     CDS             <1..1366
                     /locus_tag="VFA54_16500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS52472.1"
                     /translation="PPGTSLQESTRVLEQIENFIKETPEVESYSLRLGAQLGLAVTEP
                     NTGDFLVKLKTRRTRDLDEVINELRQKINRAAPVIDIEFPHVVEDLVGDLTWSPEPIE
                     VRLFTENLEIGRPLATQIAQFLRKVPGVVDVVNQVEDNVIGPQVDFKVDREKAERAGF
                     AVADVAQIEAAVMDGVEASTMIEGERLIPLRVRYPASYHLTREQLENLPITSANGVTV
                     PLRSIAETRIESDVPEIWRENLRRVFTVTARLSGRDLGSAMREIQQRLPREVNLPPGL
                     DLEYGGLYRVQSESFRTLTGVLLMSILLIFIILLFEFQTFSHPIAILAATLLCSFGAL
                     LALFLTKTTLNISSFMGAIMVVGIVHKNGILMLDSEKHYTERGLPLVDAIFQAGRRRL
                     RPIVMTALATIFGMLPLAIGLGSASRMLQPLAIAVIGGVSISMILSLLITPVLFYLLR
                     SKGW"
     gene            complement(1486..1641)
                     /locus_tag="VFA54_16505"
     CDS             complement(1486..1641)
                     /locus_tag="VFA54_16505"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HZS52473.1"
                     /translation="MLLTILLIILVLAMIGSLPAWPYSTGWGYYPSGAVGLVLVIVLL
                     VLLFGRA"
     gene            1995..2498
                     /locus_tag="VFA54_16510"
     CDS             1995..2498
                     /locus_tag="VFA54_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52474.1"
                     /translation="MEQTREEQQSDPKRQMLRHTVATLAYRGAKALRDAPGGFAQFQL
                     AETTRTPGEILAHVGDLLDWALALAQGRQDWKDSPPVEWEAEVRRFFGALDALDLYLI
                     SSSALHCTPEQLFQGPVADALTHIGQIAMLRRLAKSPIRGENYSRAQIQAGRVGPNQA
                     EPVREFD"
     gene            complement(2657..3085)
                     /locus_tag="VFA54_16515"
     CDS             complement(2657..3085)
                     /locus_tag="VFA54_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HZS52475.1"
                     /translation="MKKQLFVLCLALLAAAGGFWLGRATTAQAAGKNRIFEIRTYTAN
                     EGKLDALHARFRDHTMRIFKKHGMTSVAYFSPLDEPLSKNTLIYILAFPSREAAKKSW
                     DDFRNDPEWKKVAAESEANGRLVSHVDSKFVEPTDYSPMK"
     gene            3535..6012
                     /locus_tag="VFA54_16520"
     CDS             3535..6012
                     /locus_tag="VFA54_16520"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS52476.1"
                     /translation="METRSNQQNKRLFDNRRVAILIVAGIFFVSCKLHAQVAGSISGY
                     VRDSSGAVLPRASVKAVSNEQQLTRTTISDQTGFYNLLAMPPGAYDITVESPGFETQT
                     QRGVRLTMSQNLRLDFVLRLGAVQQEVTVASQATLVDTTSQTLSSLVDDRRVQDLPLN
                     GRNIMSLAKILPGILNVNAPQELANTRGGPNMSVNGGRSVDNNYIFNGANFTHFGQTT
                     GMNYPPPDAIQEIRVQTHNFSSEFGNNAGSQVTVTSKSGTNQFHGTAWEFLRNDKLNA
                     RSFFQPRRPTSRQNQAGAAAGGPIKKDKLFAFGYYQKLWNRPEVGSTVTIVPTDAQRA
                     GDFSGSSVRLRNPTDGLTGQPWIAPTGAPCVVNNIINPGCISPAAKTVLDRFIPHSAT
                     GNYVSFSPQPSSNFSYMTRIDFLQSQKHTLFGHYFQDSYRQIITAGNIQPFVTGHRSV
                     DTHNFSITSTYTFSPTLLNEATFDFLHTASLDDPDAQYTPKSLGINIPDGVNGEGISI
                     NAQGSFNLGAVNPNQQKYKNWHFRDSMSWIRGRHTFKWGYEMHYVDWVLNDKLTQTRS
                     ATFSGARSGDPIADFLLGAFDQVSVVFGQPGSYPVGWKHFFYGQDEFKVHPRFNLTIG
                     VRWEPFFPWDQKYHQQTSTDIGGGLRARSVVHPDSIPGVLFPGDPGLPSNGKLTFNDM
                     NNVGPRVGFAWDVFGNGKTSVRGGYGIFFNQISANDVHTTEAPFAGTDLLRNGRLDDP
                     YGSLNRPLPPTGKLSGNFGCVPINSFPGVHCNFPLPANLVTTDPHLVTPYVQSMNLTI
                     ERQIGKDVRVEVSYVGALSQKLEGHRH"
     gene            6058..6885
                     /locus_tag="VFA54_16525"
     CDS             6058..6885
                     /locus_tag="VFA54_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52477.1"
                     /translation="MAQNANERVLYPQTLGLLNPQCRLLGNDYRAGYHSAQFRVDKRF
                     SKGFSLLGAYVLSKNIDDVVAPEPGLTPGVDDPLNIRLDKGRGNFDRRHAVSVSWLWS
                     PQTQFSNVWERRLLGGWSIAGFHAIQSGAPLNFNMGTDVAINGTGQQALQHAQLANGI
                     TYNDIALSHPDRNSFVDQFFNTKAFVPVSQLPLGLYGNAGRNILSGPALNNSDFTVMK
                     EITAREPLRVQLRGEFFNAFNQVNFDPPNVQVSSSSFGKIRSAQPGRVIQLALKLLW"
     gene            7281..8300
                     /locus_tag="VFA54_16530"
     CDS             7281..8300
                     /locus_tag="VFA54_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZS52478.1"
                     /translation="MVFAGAALITGCSTAPAPSKGTETQSAGTAGTGSIVRLDPALDA
                     LVPKDARIEKLAGGFTFTEGPLWRPAGYLWFSDVIGNVVRQWSPDGKVTEILRPGGYD
                     KNDAPPGSYIGPNGMTADRDGAVLLCQHGNRRIVRITADRKVSTFLDRYEGKRFNSPN
                     DLVYRSDGSLYFTDPPYGLAKGDADPAKEIPFNGVYRFAGGKLQLVIRDLTRPNGIAF
                     SPDSKILYIANSDETHKVWMRYDVAVDGAVHNGRVFADVTAEKEDGLPDGMKVDSLGN
                     LYASGPGGIWVFSPDGKHLGTIKPPETPANCAWGDDGKSLYITARTGLYRIKLAVAGE
                     KMLYQ"
     gene            8323..8712
                     /locus_tag="VFA54_16535"
     CDS             8323..8712
                     /locus_tag="VFA54_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52479.1"
                     /translation="MKRRTCLKTMLAGAAARVIRGAEASRPIQLHLDLAVDPAREQEM
                     LHNFRTIFRPAASKQPGYIDVKMLKLRSTLQGAAPAGGNYRFVLTYASEELRQKWVNS
                     EVHKKVWPTIENTLKSKDYSVLLYDTY"
     gene            8852..9745
                     /locus_tag="VFA54_16540"
     CDS             8852..9745
                     /locus_tag="VFA54_16540"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZS52480.1"
                     /translation="MRILYVAFLLVFPLIAREPLAQRIAHTDPAKYRPSKAVHDGAGE
                     MRYMALFDSHALDTNLYFLHRGVIEPKSGIGHHFHNQCEEMFVIFDGEAQFTIDGRTS
                     QLKGPAGAPCRMGHSHAIYNPTDRPVQWMNINVSALKGEYDTFNLGDARVDAPLDRIP
                     VFMTMYLKRDLLRPVENMDGGKGTVQYRRALGPSVFAGPWAYVDHLVLPPGTSTGPHM
                     HRGVAEFYYVISGDGIARVGTGRQPPESAAIHPGDAVPVQLYEVHSFENTGAQPLEFL
                     IVGVARDNGKAIDVVDIPDSR"
     gene            complement(10085..10483)
                     /locus_tag="VFA54_16545"
     CDS             complement(10085..10483)
                     /locus_tag="VFA54_16545"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HZS52481.1"
                     /translation="MSTLERNRQVIRAYVEAFNRCDVDKLRSLFTPDAIIQGVLGSAP
                     IDAAIAVWRELHQAYGISLDIEEIVIEGDVAAARYSESGTFRKPFRGMEPTQKSYSII
                     AMEWFQLRDGLIAKRWGARDSAAINRQLGI"
     gene            complement(10759..10911)
                     /locus_tag="VFA54_16550"
     CDS             complement(10759..10911)
                     /locus_tag="VFA54_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52482.1"
                     /translation="MAAVGAVSQKIGNRALTGLQQRPLETGGRLVKQTRGIWRMLAES
                     HLTRRL"
     gene            complement(10918..11121)
                     /locus_tag="VFA54_16555"
     CDS             complement(10918..11121)
                     /locus_tag="VFA54_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52483.1"
                     /translation="MGASFFIEEIETGLRRFYTFLLLRRGFALADLFLQSDYSRIAGD
                     EDVDDAERLAQDPTFRLIGSEKT"
     gene            11339..13018
                     /locus_tag="VFA54_16560"
     CDS             11339..13018
                     /locus_tag="VFA54_16560"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZS52484.1"
                     /translation="MLKRTAVFTLLLLLICAAGHAEEPFLLHVMKEELNRSFSILKQK
                     ADPPPYFISYEISEQQTYSLSATLGALVTNTQSHSRLLDVTVRVGDYRLDNYHRLRGQ
                     AGFGGAGGGGALTISLDNDPDSIKASLWAQTDRKYKLAAQRLIRIKTNRAVKVDEEDS
                     SDDFSREEPVVFAGPRAPLNFSPQPWAVRLKKLSAAFRSHPQVLESKLILHVESLNKS
                     LVNTEGTAVEHGRNFLRLMITARARADDGMDLDRFESFEAADPSGFPPDDQILAVIET
                     MARELEQMRKAPPAEPFVGPAILSGRASGVFFHEILGHRVEGHRQKDEEEGQTFAKKL
                     GEPILPPFLSVIFDPTLKKHNGIELNGWYDFDDEGVKARPVTVVDQGVLKTFLMTRSP
                     IASVSRSNGHARRQPGREVVSRQSNLLVESTRRVSDEELRRMLIAEIRRQNKPYGLYF
                     RDIQGGFTTTSREGVQAFKVMPLVVYRVYADGRPDELIRGADIVGTPLAAFGKILATS
                     DRTEVFNGYCGAESGSVPVSAISPAILVSEMEIERQRTAQDRPPLLPPPEN"
     gene            13018..14805
                     /locus_tag="VFA54_16565"
     CDS             13018..14805
                     /locus_tag="VFA54_16565"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZS52485.1"
                     /translation="MRRILLLLLIACLCRAQSSSPAETDVILRAASDEMARSMRDLHL
                     ESLPRPYYIDYMVLDSDSFNAAAVFGGLARSQRIHSRVQVVNVRVGDYQLDNTNFVGG
                     SFAHSSNYDVYTLPLDDDYFVLRRHFWLSTDEAYKAAVESLARKRAALRNIRLSLQLD
                     DFAKQPPTQSIGSRGRLVIDQKAWEERVREFSALFRRFPKILSSRVVLEASAGTRYFL
                     NSEGTRVREPEAIYLLQAFASTQAPDGMQLYDSVSIPARSQELLGAPEQIRAQIEAMA
                     RRLSEFQSASTLDGYSGPVIFEGTAAGQVFAEALGRNLALTRRPLSEGGRGTLFGVPS
                     GGISGALDGRRKSRILPEWMDVVDDSTQAQWQGKLLLGHYNVDLEGVKPVRVELIHRG
                     VLENYLLSRLPVRGFEGSNGHARLPGPGGTSLASPGNLFVIAHQTVPEADLKKQLIEM
                     CRVQDREFGLLVRKIDFPSSAPREALQNIFSGQSGEDRPTSIPLAVYKVYASDGHEEP
                     VRGLRFRGFSVRNLKDIIAAGDQPFVFDYIDNGGPFALMEGAYGAETSVIAPSILVDD
                     VELKGSEEQLPGLPVVPHPFFTRAARQPN"
     gene            complement(14826..16970)
                     /locus_tag="VFA54_16570"
     CDS             complement(14826..16970)
                     /locus_tag="VFA54_16570"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS52486.1"
                     /translation="MVDLSRLACNGAAGKPHRVTRGRLAWLRLAAICLACCFFLRAGD
                     DLRALATIDGASGYEGSVLDYVRKAVGGRQEVDNTGSLTAAFGAGRPHTLFIAGLDEP
                     GYLVSAITSDGYLRVYRLSPNPPHPDFDRFFLAQPVRVTTASGRAINGVVAGLSVHLQ
                     TERAAAAKTEHPEQMYVDIGARSEREAREAGVDLLDPITLEKSYTELGSGKRMSAPWI
                     SARAGAAVLIALAERMRQAPPSNAVTLAFVSQQYGGNQGLARVLERVAADRVIWIKPG
                     GAPHPAIAPVSYRTSEMADILAALAEKRKLEFDRAAVEKVLVPAFANEEIWKNPSQAA
                     AVTLGVENAGTPVETVAQANLDAVESLLAEFGGIRAGGAPRPAVAAPVSRPPAFTLET
                     LLNAAGVSGHEAPIRETIQNLLPAWARQASRVDEKGNLIIKLGEKPERLFLSHMDELG
                     FEVVGIQPEGSLLVETRGGGISDFFEWHPAVVHTGSRPLPALMLGPEGEAGGASLRSI
                     VEVGAATAEEARAMGIKTGDTVTARKRLRPLLGSRTSSRSLDDRIGCAILIETLRSIR
                     REDIKRPTWFAFTAAEETGLHGAEFLAQSVHPAEVYAIDTFVSSDSPLENQRFAAARL
                     GGGFVIRAMDSAGMTPRSAVARLAELARRHGIPVQYGVTSGANDGSRFVSGGALNIPI
                     GWPIRYSHSPAEVADMADVDALGKIVRLLAIN"
     gene            complement(17040..17510)
                     /locus_tag="VFA54_16575"
     CDS             complement(17040..17510)
                     /locus_tag="VFA54_16575"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZS52487.1"
                     /translation="MSAKNNRRNFLKAAGAGAALAGAARARAAETAPVKKVHDRARPS
                     GSKAAQPPLFSGAVSYGNLLFLAGIGAHFAGDIKAHTKHVLDEMEKALVNAGSSMEKV
                     LKVNVYLNDLKDYQAMNEVFRGRFGPEPPVRTTIAAAGGIPGNSLVEIDCIAYI"
     gene            complement(17542..19185)
                     /locus_tag="VFA54_16580"
     CDS             complement(17542..19185)
                     /locus_tag="VFA54_16580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZS52488.1"
                     /translation="MNGRPPTAIAWIFAALLAYALGAVPGAAPSVDVPVGRLSAERMR
                     SDVTFLCSRALAGRLSGEPGSQSAADFVAAAMRNAGLQPVAGAGSYFQQIPLLEFEID
                     DSESALGWLFQKQPGLSTVFHPPGNFFCRFPKSAETEAGVVFAGYGITAPELHYDDYA
                     GLNARGKFVLALAGEPQRERGDSVFNGRGDTLYAGSHAKLLNAAKHGAAGLLLAAGRS
                     SARIRASSGKAMEPNVPAQALAEEETGIPLVSIASHVADDLLRAAHKQRADLESRIDA
                     ALRPQSFELAGIRMTLNHRLLRSRAVSSVNVAAWIEGSDPKLKSETVIVCAHYDHLGA
                     AGGQYFPGANDNASGTAAVLELARAFAGSGARPRRSLLFIVFGSEEEGLLGSFYYVRH
                     PLRPLEGTVAVLDLDMIGRNETPGPETDGRWHIPADTANVLDPVGTHYSPDLRRAMEA
                     ANGRVGLKLDFRLDADHSLDIMQRCDHYPFALEHIPAVWLFGGFHPEYHQVTDTPDRL
                     DFTKMLKVTQLTMRLAWELANQDGRPKFILRPGPVKETA"
     gene            complement(19182..19940)
                     /locus_tag="VFA54_16585"
     CDS             complement(19182..19940)
                     /locus_tag="VFA54_16585"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS52489.1"
                     /translation="MKLKDRIAVVTGGGTGIGRAISHLFAREGATVVVNYSQSQAEAE
                     EVARDICANGGNAAAYRADVRREADVKGMIAEVERRWGRLDVLMNNAGWSTRVPHHQL
                     DALTDEIWDRTFDVNLRGLFYCVRAAVPLMRKQKGASIVNLVSSAAFHAAGSSMAYAA
                     AKAGALSLTKSLARVLAPDIRVNAVCPGLVRTRFAGWPEEAFEHGAKVTPLGRLATVE
                     EVAQAALFLAADATAMTGEALLVDGGLYQLGRSR"
     gene            complement(20062..21393)
                     /locus_tag="VFA54_16590"
     CDS             complement(20062..21393)
                     /locus_tag="VFA54_16590"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZS52490.1"
                     /translation="MTRRKLLLGMAAAAAGAPAARGAGTDWTKASQEAIGRLREYIRI
                     DTSNPPGNVAGAVSFLAGVLQKEGIQSERLEAAPGKVNLLARLPGNGSKRALLLLNHT
                     DTVPADRSRWQTDPWGAEIQLNRLWGRGAVDMKSTGILHLMAFLLLKRNRVPLKRDVI
                     FAATADEEVGGGSGAKFLLDKYPQKVESEYVLEEGGFSANRLFTPEGDVYGVSVAQKQ
                     VLWLHLTVEGTGGHGSQPADDNAIAMLSGVLRRIVETAPPKRTDPILDQMLTRLGTLA
                     DNRFANSMRRNSIALTSFRAGVGDPPKDNVVPSIATASIDCRLLPGQPVDEFIEWVRK
                     TGAEPKLKIENVHHQPSQPPSPADTELFRLIETTVAREAPNAKVTPYLTPFGTDGNFF
                     RRPDRYVYGFFPVVLSAEEVMSMHSDAERMPVAPFEKGLRMFYEVVAGIAG"
     gene            21599..21934
                     /locus_tag="VFA54_16595"
     CDS             21599..21934
                     /locus_tag="VFA54_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52491.1"
                     /translation="MLRINSWRGAGAVLWLLTFGISSPAQRRGRWEYLGEANVDGALD
                     HDNIVVTGARGKYRAIQIRVERAPIEFDRVIVHYGNGSAEPIPIRRGIPAGGPTRAID
                     LPGDRRSRA"
     gene            22290..22382
                     /locus_tag="VFA54_16600"
     tRNA            22290..22382
                     /locus_tag="VFA54_16600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22324..22326,aa:Ser,seq:gct)
     gene            complement(22489..24879)
                     /locus_tag="VFA54_16605"
     CDS             complement(22489..24879)
                     /locus_tag="VFA54_16605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52492.1"
                     /translation="MHLYTAAAGGITVMGSVSVVIPCYNPTRFLKETLASVRAQTYGR
                     VEIILVDDGTDHPEARKLLKSAAGGADRYIEQSNQGLAAARNSGFRAARGDYVVPLDA
                     DDRLDPAFVSECVREARAHPEAAFIYTDYRVFGEKQYVERLDDYNLFRLLDQNTLIYA
                     SLIRRSDWEAAGGYDDSMRLGYEDWDFWLRLAERGRYGRHLPRALFEYRKHGRSLLTV
                     AREHHAELVAKIRANHPQLYSPEGRARIKAQWAPAVCIVGRAVAAAQTIKDVQEQPAS
                     LPGTALERSRAPAFLIPPAGEIDAHSAELCALAVWSGRAATRLPDGCLCASRRTLERI
                     NDFREIAEKIESAGVPCAARPAAGRRVWELVRRHLVNAELLSLSSWLRHPLQSAGRLI
                     PLRIKESINRAAGRSVFDLSFYLKFQPKSILALGQLVEPLCYMPRPADRRRIALLTPH
                     LGPGGAESVLLEAAGAIDRAACEIFLIATQSHDSRWEQRWRQRTDHVYDLARFVGSDR
                     MIAAICSIVRNWQFHTIVIQNSLAAYSAIPHLRSLLPPLQIVDWIHAVDRAWDFVSAS
                     APVASEIDLRIVISDAARRRVRAAGVAAEKIWLIRNGVDLERFRPAPFPGAAVKNILF
                     AGRLDPIKRPLLLADIAAELVRLRPARDFHFVVAGDGEQRSALEARIRTGGIEALFSL
                     LGHVEEMPAVVAGCDLVVVPSQAEGIPLIVLEALASQRPVICSRTGAIDEVVDEHNGV
                     LVEPGRREAAEFAKVIDALLSNAGRRREMGEAGRKKIEAEYNLERARQAYRQLFLL"
     gene            complement(24840..27365)
                     /locus_tag="VFA54_16610"
     CDS             complement(24840..27365)
                     /locus_tag="VFA54_16610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS52493.1"
                     /translation="MPFPPLVSVVIPCYNPSRFLEEAIDSVRAQTYSPLEIILVDDGT
                     DRLAACQYLELARPRVDRFIRQTNRGVSAARNAGFRAAAGTYVLPLDSDDRLKPNYLA
                     ECVPLLEAHPEAAYAYSEYQVFGDVEYIQRAADFNLYQLLDHNTVSAASLVRKADWKL
                     AGGYDESMRDGYEDWDFWLRLAEQGRYGRYVPKVLYEYRKHGPSLYSAARERHEELVA
                     RIRANHPRLYSREGILQAKICWEPAVCILAAEEGLVPARQTIEDVQVLCETDPARALA
                     ASRAPAFLIPALEGITDPQSAELCALAVFSGKSVVRLPDGSLGLARGMLSGVASLAAI
                     EQHAPTRQVPRPESLCYLTRPTPPARDGSSKRRVALIVPHLGPGEQHNLLLKLADSVD
                     RDRCDLFIIALEANRSEWRDRWRQITPHVYNLAPLIEPAEMTAAYYWFVVNWKIDVLV
                     IQNCPPGLSIVPFLRERLPAVRVIELIHESAEAPEGVAFLAEADPDLRLVISKAGRRW
                     LLESDAWMDLEAMIAAAAKAPASGKTLLLGETSIEDLSAAPDCFSPGSAPVPLPQIDD
                     VALHFADQLQVTGLRFSEKCGVFVLETRWRCLKTPERRWRCFAHIVNPQGALLGSMDH
                     EILRGRPNLTSWRPGDEGYESRCLLLPPSCAEGARLHFGLFDPEAQMRATLWASTVPV
                     RDLYTAAVFEPNRKPPADYRFRLEPHPMERCGVEFANGVRLTGYSFARAADVLRLRLQ
                     WETLHRSEEEKLYFFGHAVAEQTADSPILLSFDQAFRMLENSASQDILREASRLSAEA
                     RFLRAGLCDLARNYERIAIRSSTLRFSEADRCIYIPLPPAASR"
     gene            complement(27369..>28199)
                     /locus_tag="VFA54_16615"
     CDS             complement(27369..>28199)
                     /locus_tag="VFA54_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52494.1"
                     /translation="NWLQVAGLRLTERDGIFTLALRWRCLTPPPMPLRCFAHVVDSEG
                     KLLSSMDHEILHGSPPVTEWQPGDEGYEARYLALPPATARGAQVRLGLFDPESGRRVP
                     LWASTAPLKDDYTAAVFLPNQPPAAEYQFQMGPAPLIACNVEFEGGIQITGYSLRRQG
                     GVAWLRLRWSAGRRPKAKLYFFGHAVADQSAGAPILLSFDEDTELNRTVRSRDGQFTL
                     IQDIVRDVSKLSDQAKFLRAGLFDIDRPLDRLAIRSSSLTMSKQQKAIFLPLPPRSSP
                     "
BASE COUNT         5573 a         8654 c         8502 g         5470 t
ORIGIN      
        1 gccgccgggc acttcgttgc aggaatcgac gcgcgtcctt gagcagatcg aaaactttat
       61 caaagagact ccggaggtcg agagctattc gctgcgtctg ggagcgcagc tcggcctggc
      121 cgtcaccgag cccaacacag gcgatttcct cgtgaagttg aagaccagac gcacccgcga
      181 tctggacgag gtgatcaacg agcttcgcca aaaaatcaat cgcgctgctc cggtcatcga
      241 tatcgagttt cctcacgtgg tggaggattt ggtcggggat ctcacctggt ccccggagcc
      301 gatcgaggtg cgtctcttca ccgagaacct cgagataggc cggccgctgg cgacccaaat
      361 cgcccagttt ctgcggaagg ttccgggagt agtggacgtg gtgaatcagg tcgaggataa
      421 cgtaatcggc ccgcaggtgg acttcaaggt agaccgcgag aaggccgagc gggcgggctt
      481 cgccgttgcg gacgtggccc aaatcgaggc tgcggtgatg gacggcgtcg aagcatccac
      541 gatgattgaa ggcgagcggc tgattcctct gcgtgtccgc tacccggcca gctatcatct
      601 gacccgcgag caactggaga atctgcccat cacgtcggcc aacggcgtca ctgtgccgct
      661 gcgcagcatt gccgagaccc ggatcgaatc cgatgttccc gaaatctggc gggaaaatct
      721 ccggcgcgtg ttcaccgtga cggcgcggct ctccggcagg gacctgggca gcgcgatgcg
      781 ggagatccag caaaggctgc cgcgagaagt caaccttccc ccggggcttg atctcgaata
      841 tgggggcttg taccgcgtgc aatcggaatc gttccgcacg ctcacgggcg tgctgctgat
      901 gtcgattctg ctgatcttta ttattctgct gtttgagttt cagacgtttt cgcatccgat
      961 cgcgatattg gctgcgactt tgctgtgcag ttttggggcc ttgctggcgc tgttcctcac
     1021 aaaaaccact ctcaatattt cttccttcat gggcgcgatc atggtggtgg gcatcgtgca
     1081 caagaacggc attcttatgc tcgattcgga gaagcattac accgagcggg ggctgccgct
     1141 ggtggacgcg atttttcaag ccggcagacg ccgcctccgc cccatcgtga tgaccgctct
     1201 cgccacgatt ttcggtatgc tgcccttagc cattgggctt ggctcggcct cccgcatgct
     1261 gcagcccctg gccatcgctg tcatcggcgg cgtgtcgatt tcgatgatct tgtccctgct
     1321 gattacaccc gtgcttttct atttgctgcg cagcaaaggc tggtaacaac tcctctgccg
     1381 cgaatctgaa actgcctgct ccacaggtcg gacgcccgtt cccacaacag gcgcaggggc
     1441 cgggagacgc cgattgcgct aaatgcgcgg cccggcgccg cgcatttagg ctctgccgaa
     1501 gagaagcacc agtaagacaa tcacgagcac aagccccact gctccgctgg gataatagcc
     1561 ccagcccgtg ctataaggcc acgccggcaa agacccgatc atagccagaa cgaggattat
     1621 cagcagaatc gttaaaagca ttaccgctct ccttttgctc ccaacattgg agcaagcggc
     1681 gtgccaagcg cgatatgccg cgccctctca aagccatgcc cgcggagcgt gcgatcggaa
     1741 actcagaaaa ctttactcat gtactcttgc tgccgatcac atttcggaca agccgccgca
     1801 agctgtgtgc gagcggccct agagaattgc cgcggctata atgagccggc gcgcgaggcc
     1861 atctgtggaa tcgaatgagc gaaatccgcc gcgggcaaag tccgctcgga tccggccctt
     1921 cagaccgcgc tttcacgcat ctgcgcctca cgattgtcct tgcaaccgcg gctcccattt
     1981 cgtttaatga gcctatggaa cagacgcgag aagaacagca gtccgacccc aagcgccaaa
     2041 tgctgcggca taccgtcgcg acattggcct accgcggcgc caaagcgctg cgagacgccc
     2101 ctggcggatt cgcccaattc cagctcgctg aaaccacgcg cacgcccgga gaaatcctgg
     2161 cccacgttgg ggatttgctc gactgggcgc ttgccctggc ccaggggagg caagattgga
     2221 aagattcacc gccagtcgaa tgggaggccg aggtgcggcg tttctttggc gccctggacg
     2281 cgcttgacct ttacttgatc tcgagttccg ctctgcactg cacgccggag caattatttc
     2341 aagggccggt ggcggacgct ctgacccata tcggccaaat cgctatgctg cgccggctgg
     2401 cgaagtctcc gattcgcgga gagaattatt cccgagcgca aatccaggcg ggccgcgtag
     2461 gaccgaatca ggctgagcct gtccgggagt ttgattgata cgatcggcgg cggaagaggc
     2521 cggccctgca cgcccatgct gtgagggctg ttctaccttg agccccgaaa tgggtctacg
     2581 gcgcggcaac ccttcggatc aactcaatga ttggcgccgc gccgtggccc gttcctactt
     2641 ggatggcggg tctatctcac ttcatgggcg agtagtcggt gggttccacg aattttgaat
     2701 ctacgtggct cacaaggcgg ccattcgctt ccgactcagc cgcgaccttc ttccattcag
     2761 gatcgttgcg gaaatcatcc caggatttct tggcggcttc gcggctcggg aaggccaata
     2821 tatagatcaa cgtgtttttc gagagcggct cgtcgagggg agaaaagtaa gctacgctcg
     2881 tcatgccgtg ctttttgaag atgcgcatcg tgtggtcccg gaaacgagca tgcaaggcat
     2941 cgagcttgcc ttcattggca gtgtaagtgc ggatctcgaa tatgcggttc ttgccggcgg
     3001 cttgagcggt ggtcgctcgc cccagccaga atcctccggc agcggcgagg agagccagac
     3061 agagcacgaa gagctgcttt ttcatattgt ggtcctttcc aaagagctga aaccattcca
     3121 tcgagcagga tacagccacg tcggagccga tagcaaggcg gagcgccgtt tttgaatagc
     3181 caccgtttcc agtattcgcg ggatgttcgc gccgagtcga ccccgcgcca agagaagcgg
     3241 atctgatccg aacctgcccg attttgcacg tcagcaaaat ggccgcccta aatttcgagc
     3301 cgctggccgg caatctggcc tgaaacgcgc cgagccaaac aagcacgaaa gtcgttgaca
     3361 gggcgggggg gcagttgtac acttcgccga aaaagcttcg gaatccggtc cccaaggact
     3421 gaatcaccgc cgggacatgg cgggcgttgc ttgtgctgga gcgaaaacgg agagtttttg
     3481 gttaagggac gcaaaagtgg ataaccgccg ggcgtattag aaaaggggtg agcgatggag
     3541 actcgatcaa atcagcagaa caagagacta tttgacaata ggcgcgttgc gattcttata
     3601 gtagctggaa tcttttttgt ctcctgcaag ttgcacgctc aagtagcggg cagtatcagc
     3661 ggctacgtga gggacagttc cggggcggtg ctgccgcgag cgtctgtgaa ggccgtgtcc
     3721 aacgagcagc agctaacgcg gaccacgatt tccgatcaga caggatttta caacctgctt
     3781 gccatgccac cgggcgctta cgacattacc gtcgaaagtc caggattcga gacgcagacg
     3841 caaaggggcg tccggctgac catgagccag aacctgcggc tcgattttgt gttgcggctc
     3901 ggggcagtgc agcaggaggt gaccgttgcc agccaggcta cgctggtaga tacaaccagt
     3961 cagacgctct caagtctggt cgacgaccgg cgtgttcaag atcttccgct caacgggcga
     4021 aatatcatgt ctctggcgaa aattctcccg ggaatcttga atgtcaatgc cccgcaggag
     4081 ttggccaata cacgtggcgg ccccaatatg agcgtgaacg gcggccgctc cgtggataac
     4141 aattacatat tcaatggcgc aaatttcacc cacttcggcc agacgaccgg gatgaattac
     4201 ccgcccccgg atgcgatcca ggagattcgc gtccagaccc ataatttcag ttccgaattc
     4261 ggtaataacg ccggcagcca ggttactgtc acctcgaaat ccgggacgaa tcagttccac
     4321 gggacagcct gggagtttct acgcaacgac aagctcaacg cgcgcagctt ctttcagccg
     4381 cgccgtccga caagccgcca aaatcaggcc ggagcggccg caggaggccc tataaaaaaa
     4441 gacaagctct tcgcattcgg ctattaccaa aagctttgga accgtccgga ggtcggatcc
     4501 actgtgacta tcgtacccac cgatgcccag cgcgccggcg atttctcagg ttcctcggta
     4561 cggctgcgga atcccactga cggtctcacg gggcagccgt ggatcgcccc caccggcgct
     4621 ccctgcgtgg tgaacaacat cattaatccc ggctgcatca gcccagccgc gaaaaccgtg
     4681 ctcgatcgat ttattcctca ctcggccact ggcaattacg tcagcttcag cccgcagcca
     4741 tcgagcaatt tcagctacat gacgaggatc gattttcttc aaagccagaa gcacaccctc
     4801 tttggccatt actttcagga cagttaccgg cagatcatca cggcgggaaa cattcagccc
     4861 ttcgtgaccg gccatcgcag cgtcgacact cataatttca gtatcacgag cacctatact
     4921 ttcagtccca ctctgttgaa cgaggcgact ttcgattttc tgcacaccgc ttcgctggat
     4981 gatccggatg cgcaatatac gccgaaaagc ttggggatca atattccaga cggcgtgaat
     5041 ggagaaggga tctccatcaa cgctcaaggc agcttcaacc tcggcgcggt gaacccgaat
     5101 cagcagaagt ataagaactg gcactttcgg gattccatga gttggatccg gggccggcac
     5161 acgttcaagt ggggttatga gatgcattat gtcgactggg ttctgaatga caagctcacc
     5221 caaacccgaa gcgctacctt tagcggcgct cgctcgggcg accccatcgc cgacttcctt
     5281 ctgggcgcat tcgaccaggt aagtgtagtt ttcggccagc ccggcagcta cccggtcggc
     5341 tggaagcatt tcttttacgg ccaggacgag tttaaggttc acccgcgatt caatctgacg
     5401 atcggtgtgc ggtgggagcc gttcttcccc tgggaccaga agtatcacca gcagacttcg
     5461 acggatatcg gaggcggact ccgggctcgc tccgtggtgc atccggattc gattcccggc
     5521 gtcctgtttc ccggcgaccc cggactgccc agcaacggca agctcacttt caacgacatg
     5581 aacaatgtag ggccgcgggt cgggttcgct tgggacgttt ttggcaacgg caaaaccagc
     5641 gtgcggggcg gctacgggat cttcttcaat caaatcagcg ccaacgacgt tcacaccacg
     5701 gaagcgcctt ttgccggcac ggatctgctg cgcaatggcc gactggacga tccgtatggg
     5761 tccctgaacc ggcccttgcc gcccaccggc aagctgtcgg gaaacttcgg ctgcgtcccg
     5821 atcaactcct tccccggagt gcactgcaac tttcctctcc cggctaatct ggtgacgacc
     5881 gacccgcacc tggttactcc gtatgtgcag tcgatgaatc tgacgatcga gcgccagatc
     5941 ggcaaggacg tgcgcgtcga ggtgagttat gtcggggcgc tctcgcagaa gctcgagggc
     6001 caccggcact agaatcccgc gatctttcaa aattccttgc ttaccggcgc tccacccttg
     6061 gcccaaaacg ccaatgaacg cgtgctgtat ccgcaaactc tgggactgct caacccgcag
     6121 tgccgcttgt tgggaaatga ttatcgggca gggtatcaca gcgcccagtt ccgcgtggac
     6181 aaacgcttca gcaaaggatt ttctttgttg ggcgcttatg tcctctcgaa aaatatcgac
     6241 gatgttgtgg cgcccgagcc tggtctcaca ccgggtgtag atgatccctt gaatatccgg
     6301 ctcgataagg ggcgcggcaa tttcgaccgc cgccatgcag tctcggtgag ctggctatgg
     6361 agtccccaaa cacaattcag caacgtctgg gagagaaggc tgctcggcgg gtggagcatt
     6421 gcaggctttc acgcgattca aagcggagct ccgctgaatt tcaacatggg caccgatgtc
     6481 gctataaacg gcactggcca gcaggcgctc cagcacgcgc agctagccaa cggcatcacc
     6541 tacaacgaca tcgcgctgag ccatcccgac cgcaactctt tcgtcgacca gtttttcaat
     6601 accaaggcgt ttgttcccgt gagccagttg ccgctcggtc tctacggaaa tgcgggacgc
     6661 aacatcctca gcggtccggc cttgaacaac agcgacttca cggtgatgaa agaaattaca
     6721 gcgcgggagc ctttgcgtgt acagcttcgt ggcgaatttt tcaacgcatt caatcaggta
     6781 aacttcgatc cgccgaacgt gcaagtctcg tcaagcagct tcggcaaaat ccggtccgcg
     6841 cagccggggc gcgtgattca gctcgccctg aagctcctgt ggtaaccgag aggcccggct
     6901 gaacgtttca ggggcgaagg ctggacttgg ttcagcgggg gatggaaaag gcacatgggg
     6961 cctgcggccg cgtttcgggg ctcgcgattc tccgcctaag ccgctctgga gtacaattgt
     7021 cgcaatccag agactcggag gaacgagtga aacccggctt tcgaaatctt atcgattctg
     7081 cggcggcaac tgcggcgagg cgtttatcgg ggggcgtcga tcgaagccga cttcgattcc
     7141 agcagccgct tctggcatcc gatcaggatc gcgctcgcga actcaaggcc gtcttcagaa
     7201 caggctccgg aaacacgcat tggcagcctg aggcgccgaa atacgcccgg ggcgtctcag
     7261 cccttgctgc aaggatatta ttggtcttcg ctggcgcagc gctgatcacc ggctgctcga
     7321 cggcgcctgc gccatccaag ggcactgaga ctcagagcgc cgggacagcc ggaactggct
     7381 ccattgttcg gctcgatccg gctctggacg cactcgttcc caaggacgct cggatcgaga
     7441 aactggctgg cgggtttacg ttcacggaag gccccttgtg gcggcccgca ggctatttgt
     7501 ggttcagcga cgtaatcggc aacgtggtgc gccagtggtc gcccgacggc aaggtaacgg
     7561 agattctccg gcccggcggt tacgacaaga acgacgcgcc tccaggatct tacatcggcc
     7621 ccaatggcat gacggccgat agggatggcg ctgtgttgct gtgccagcac gggaaccggc
     7681 gcatcgtccg catcacggcg gacaggaaag tcagcacttt tctcgaccgc tacgagggga
     7741 aacgctttaa tagcccgaac gatctggtct acagatccga tggttccctc tacttcaccg
     7801 atcctcctta cggactggcc aaaggcgatg cggacccggc caaggaaatc cccttcaacg
     7861 gcgtttaccg cttcgctggg ggcaaattgc aattggtgat cagggacttg acgcggccta
     7921 acggcatcgc tttttcgccc gattccaaaa ttctttacat cgccaattcg gatgaaacgc
     7981 acaaggtgtg gatgcgatac gacgtcgccg tcgacggggc ggtccacaac ggccgcgtct
     8041 tcgcggatgt taccgcggag aaggaagatg gcctgccaga tggaatgaaa gtcgattcgc
     8101 tgggcaacct ttatgcttcc ggcccgggag gaatctgggt attttcgccg gacggcaaac
     8161 acctggggac gatcaagccg ccggaaacgc cggccaattg cgcttggggc gatgatggta
     8221 aatcgctcta catcacagcg cgtacgggct tataccggat caagctcgcg gtggcgggag
     8281 aaaaaatgct ctatcagtaa tgcgcgaaag aaagagggag ctatgaaacg gagaacatgt
     8341 ctaaagacga tgctggcggg agccgctgcc agagtcatcc ggggcgcgga agcgagccgc
     8401 cctattcaac tgcacctgga tcttgctgtg gacccggcca gagaacagga gatgttgcac
     8461 aattttcgga cgatcttccg gccggcggcg agtaaacagc ccggatatat cgacgtgaag
     8521 atgctgaagc tgcgctcgac tttgcagggc gccgccccgg ccgggggcaa ttaccgcttc
     8581 gttctgacct acgccagcga agagctgcgg cagaagtggg taaactcgga agttcacaag
     8641 aaggtctggc caaccatcga gaatactttg aaatcgaagg attacagcgt cttgctctac
     8701 gacacgtatt aagctggaat caaaaggggc ctgcaaaagc gccgcctgaa gagacgacgt
     8761 cggcacggcc agcccaaggt ctcgtgaatg ttctcgcccg gactccggag gccgcggcat
     8821 gtggcaatcc gcgggagaaa ggaaggaaga aatgaggatt ctgtacgttg cattcctttt
     8881 ggtgttccct ctgattgcgc gggaaccgtt ggctcagcgc atcgctcaca cagacccggc
     8941 caaataccgc ccgtccaagg ccgttcatga tggggccggc gaaatgaggt acatggcgtt
     9001 gtttgacagc catgcgctgg atacaaattt atatttcctg caccggggcg ttatcgagcc
     9061 gaagagcgga atcggccatc acttccacaa ccaatgcgaa gaaatgttcg tgatcttcga
     9121 tggcgaagcg caattcacga tcgacggccg aacctctcag ttgaaagggc cggccggagc
     9181 tccctgccgg atggggcact cccacgccat ctataaccca accgaccggc cggtccagtg
     9241 gatgaacatc aatgtctcag cgcttaaagg ggaatacgac accttcaatc tcggcgatgc
     9301 ccgcgtggat gcgcctctcg accgcatacc cgtcttcatg acgatgtacc tgaaacggga
     9361 tctgctccgg ccggtggaaa atatggatgg aggcaagggc acagtgcagt accgccgggc
     9421 gctgggtccc agcgtgttcg ccggcccgtg ggcctatgtg gaccaccttg tcctgccgcc
     9481 gggaacctcg actggccctc atatgcacag gggagtagcc gagttttact acgtcataag
     9541 tggagacggc atagccaggg tagggactgg ccgccagccg ccggagagcg ccgccattca
     9601 cccgggggat gcggttccgg tccagcttta cgaggtgcat tcgttcgaga ataccggcgc
     9661 tcagcccctc gaattcctga tcgtcggcgt ggcccgggat aatggaaaag cgatcgacgt
     9721 cgttgacatt ccggactcgc gctgagcgcc tgccctggca agttgtcttc gtgtgcggcg
     9781 gagcgaggcg gcgccggacg gtcctccaag aaggggcgtc gcattctctg tctcggaaaa
     9841 gatgagaacg tgaagaaatc cgatcgcgcc cgtgacgttt cgctaaatac taagtattta
     9901 gccagactta tggcctcaag ggccagcgtg ccgcgatcaa agcttatcgc gaactgatct
     9961 gccgcgcgcc tgattccgaa gcggcgtcgg ccgccaagcg gtcctttcca ctccatttaa
    10021 ttcgaatgcg ttctatccgc cgctgctgaa gttttaggga cgtttccggc gctggcagcg
    10081 gaaactaaat gccgagctgc cggttgatgg ctgcggaatc ccgcgcaccc cagcgtttcg
    10141 caattaggcc atcgcggagc tggaaccact ccatggcgat aatactatag cttttttgcg
    10201 tcggttccat tcctcgaaag ggcttgcgaa atgtgccgct ctcggagtag cgggcggcag
    10261 cgacgtcgcc ttcgatgacg atttcttcga tatccaagct aatgccatag gcctggtgca
    10321 attcgcgcca cacagcaatc gcagcatcga tcggcgcgct gccgagaact ccctggatga
    10381 tcgcgtccgg cgtgaacagg gaacgcagtt tgtccacgtc acaacgattg aaagcctcga
    10441 cgtatgcgcg gatgacctgt ctgttccgtt ctaaagtgct catacccagg atttccattt
    10501 tcggcttctg gattgggctt agcatatcac aaccccgcag tcttgatcct cccaagcgta
    10561 gctttctccg cgagccttct attgcctctg ccaagcgcca aattgcaccc acaggaaaac
    10621 ccgaaaaacc agggaagctg cgtcttttca gtgactccta caaatacatc tccggcccag
    10681 gtcttgcccg aatgatttcg ctgctgccac cgccctcctc tacccagtcg gcgcccccgg
    10741 cgtccgccgg atgctgctct acaaccgccg cgtgaggtgg ctctcggcca gcatccgcca
    10801 gatgcctcgg gtctgcttga ccagcctgcc accggtctcc agcggccgtt gttgcaaacc
    10861 ggtcagcgcc cggttcccga ttttctgcga aacggcgcca accgccgcca cacgatctta
    10921 agttttctcc gaaccgatca gtcggaacgt ggggtcctgc gcgagccgct cggcgtcatc
    10981 gacgtcctca tcccccgcga tacggctgta gtcagactgg aggaacagat cggcgagcgc
    11041 aaaaccgcgt cgtaagagca ggaacgtgta aaaacggcgc agtccggtct caatttcttc
    11101 gatgaaaaag cttgcaccca tcgcgcactg gcgcgcagta tcgagaatcg cgatcgggag
    11161 ttgcagccag ttctttcccg aaggcgttca gcgggcgtcc gtccatactg tgttatccta
    11221 gtgccgttcc ggctgcctca gctcggcgtc gaggggccgc tgggtggctg agccgccttt
    11281 ataagacccc ctgcggagcg acggagtggt tggaacgtgc gagcgcgaga ggtacagttt
    11341 gctgaagcgg accgcggttt tcacgctctt gctgcttctg atctgcgcgg ccggtcatgc
    11401 cgaagagccg ttcctgttac acgtgatgaa ggaggagttg aatcgctcct tcagcatcct
    11461 caaacagaaa gccgacccgc cgccctattt catcagttat gaaatcagtg agcagcagac
    11521 ttacagcctc tcggcgacgc taggcgcgct ggtaaccaat acgcaaagcc attcgcggct
    11581 gctcgacgta acggtgcgcg tcggcgacta taggctggac aactaccatc gcctgcgcgg
    11641 ccaagccggc tttggcggag ccggcggggg cggcgctctt accatcagtc tggataacga
    11701 ccccgactcg atcaaggctt cgctgtgggc gcagaccgac cgtaaataca aactggccgc
    11761 ccagcgcctg atccggatca agaccaaccg agccgtcaag gtggatgaag aggattcgag
    11821 cgacgacttt tcgcgcgaag agccggtggt cttcgcgggc ccccgagccc cgctcaattt
    11881 ctcgccgcaa ccgtgggccg tgcggctcaa aaagctctcc gccgcctttc gctctcatcc
    11941 gcaggtgctg gaatccaagc tgatcctgca tgtggaaagc ctaaacaaat cgctggtgaa
    12001 tacggaaggc actgccgtgg agcacggacg caacttcctg cggctgatga tcacggccag
    12061 ggcccgggcc gacgacggca tggatctgga ccgcttcgaa agctttgagg cggcggatcc
    12121 atcaggattt ccgcccgacg accaaatcct tgcggtgatc gagaccatgg cccgcgagct
    12181 cgagcagatg cgcaaggctc cgccagccga gccttttgtc ggtcccgcca tattgagcgg
    12241 gcgcgccagc ggcgtattct tccatgaaat tctggggcac cgcgtggaag gacaccgcca
    12301 aaaggacgag gaagagggcc agactttcgc caagaaactg ggcgagccga ttctgccgcc
    12361 gtttttgtcc gtgattttcg acccgacttt aaagaagcac aacggcattg agctgaacgg
    12421 ctggtatgac ttcgacgacg agggtgtaaa agcgaggccg gtaacggtgg tggaccaggg
    12481 tgtgctgaaa acgttcttaa tgacacggtc tcccatcgcg tccgtgtcgc gttcgaacgg
    12541 gcacgcccgg cgccagccgg ggcgggaggt cgtttcgcgc cagtcgaacc tcctggtcga
    12601 gtctacgcgg cgcgtttccg acgaagaact gcgccgtatg ctgatcgccg aaatccggcg
    12661 ccagaacaag ccctacggat tgtattttcg agatatacag ggtggcttca ccacgaccag
    12721 ccgcgagggc gtgcaagcct tcaaagtgat gcctttggta gtgtaccggg tttacgccga
    12781 cggccgtccg gatgagctga ttcggggcgc ggacattgta ggaaccccgc tggcggcatt
    12841 cgggaagatc ctggccactt cggaccgcac cgaagttttc aacggctact gcggcgccga
    12901 gtccggcagc gttccggttt ccgcgatttc gccggccatt ctggtatcgg agatggaaat
    12961 cgagcggcag cggacggcgc aggaccggcc cccgctgctg ccgccgcctg aaaactgatg
    13021 cgacgaattc tcctgctgct gttgatcgcc tgcctgtgcc gcgcccagtc ttcgagcccg
    13081 gcggagacgg atgtcattct gcgtgcggca agcgacgaaa tggcccgctc gatgcgcgat
    13141 ctgcacctcg agagcctgcc gcgcccctac tacatcgact acatggtgct cgattccgac
    13201 agcttcaacg ccgccgccgt ttttggaggc ctggcgcggt cgcagcggat tcactcccgc
    13261 gttcaggtgg tcaatgtgcg ggtgggagat tatcagctcg acaacacgaa ctttgtgggc
    13321 ggctcgttcg cgcacagttc caactacgac gtatatacac ttcccctcga cgatgactac
    13381 ttcgtgctgc ggcggcattt ctggctctcg accgatgaag cctataaagc cgccgtcgaa
    13441 tcgctcgcgc ggaagcgcgc cgctctgcgg aacataaggc tctcattgca gctcgatgac
    13501 ttcgccaagc aaccccccac gcagtcgatc ggcagccggg gccggctggt gatcgaccaa
    13561 aaggcctggg aggagcgggt ccgggagttt tcagctctct tccggcggtt cccgaaaatt
    13621 ctatcctcac gcgtcgttct ggaggcctcc gccggcaccc gctatttttt gaacagcgaa
    13681 ggaacgcgag tgcgggagcc cgaagccatt tatctgctgc aagcgtttgc ctccacgcaa
    13741 gctccggacg gcatgcaact gtacgattcg gtttcgatcc ccgcgcgcag ccaggagctt
    13801 ttgggcgctc cggaacagat ccgggcgcag atcgaggcca tggcccggcg gctgagcgaa
    13861 tttcaatcgg cttcgactct tgacggctac tcgggtcctg tgatcttcga gggcacagcg
    13921 gccggccagg ttttcgccga agcgctgggg cggaatctcg ccctcacgcg ccgtccgctt
    13981 tccgagggag ggcgaggaac gcttttcggc gtgccctcgg gcggcatcag cggcgcgctg
    14041 gacggccgcc ggaaatcccg catcctgccg gagtggatgg acgtggttga tgattccact
    14101 caggctcagt ggcaagggaa actgctgctc ggccattaca acgtggacct ggaaggcgtg
    14161 aagcctgtcc gcgtggagct gattcatcgg ggggtgctcg aaaattatct tctttctcgc
    14221 ctgccggtgc ggggctttga aggctccaac gggcacgccc ggctgcccgg ccccggaggc
    14281 acatcgctgg ccagccccgg caacctgttt gtcatcgccc accagacggt gcccgaggcc
    14341 gatttgaaaa aacaattgat cgagatgtgc cgcgtccagg accgggaatt tggattgctg
    14401 gtgcgaaaga tcgattttcc ctcttcggcg ccgcgggaag ccttgcaaaa cattttctcc
    14461 ggccagagcg gcgaagatcg tcctaccagt attcccttgg cggtgtacaa ggtttacgct
    14521 tcagacgggc atgaagagcc ggtgcgcggt ctccgctttc gaggtttttc cgtccgcaat
    14581 cttaaggata ttatcgccgc gggagatcaa cctttcgttt tcgattacat cgataacggc
    14641 ggccccttcg ctttgatgga aggcgcttac ggtgcggaga ccagcgtgat tgcgccctcg
    14701 attttggtgg acgacgtgga attgaagggc tcggaagagc agttgccggg cttgccggtc
    14761 gtcccgcatc ccttcttcac gcgggccgcc cgccagccga attgaaatcg agctgttccg
    14821 gaagctcagt tgatcgccag gagccggacg atcttcccca gcgcgtcgac gtcggccatg
    14881 tcagccactt cggccggcga atgcgagtag cgaatcggcc agccgatcgg aatattgagc
    14941 gcgccgccgc tcacgaaccg agaaccgtca ttcgcgccgc tggtgacgcc gtattgcaca
    15001 ggaatgccat ggcggcgggc cagttccgcc agccgcgcca ctgcggaccg cggcgtcatg
    15061 ccggcgctat ccatggcgcg gattacaaac ccgccgccca ggcgcgccgc ggcgaatctc
    15121 tgattttcca aaggagaatc ggaagagacg aatgtatcga tggcgtagac ttcggcagga
    15181 tgcaccgatt gggccaggaa ctccgctccg tgcagccctg tttcttcggc tgcggtaaac
    15241 gcaaaccatg tgggccgctt aatgtcctcc cggcggatcg atcgcagggt ttcaatcagg
    15301 atggcgcacc cgatgcgatc atccagggag cgggaactgg tgcggctgcc caataatggc
    15361 cgcaggcgtt tgcgcgcggt taccgtatct ccggttttga tgcccatcgc ccgggcctcc
    15421 tccgccgtcg ccgctccgac ctcgacgatg gagcggagcg acgcgccgcc ggcttccccc
    15481 tccggaccca gcatgagcgc cgggagcggc ctggagccgg tgtgtactac tgcggggtgc
    15541 cattcgaaaa agtcggaaat gccgccgccg cgcgtttcaa ccagcaaaga gccttccggc
    15601 tgtatgccca ccacctcaaa ccccaactca tccatgtggc tcaaaaacag ccgttcgggt
    15661 ttctccccga gtttgataat gaggttccct ttttcatcta ctcgggatgc ctgccgcgcc
    15721 caggccggaa gcaggttctg aatcgtttcg cggatggggg cttcatggcc gctgacgccg
    15781 gcggcattca ggagcgtctc cagcgtaaaa gccggcgggc ggcttaccgg cgcggccacc
    15841 gcaggacggg gcgcaccgcc cgctcgaatg ccgccgaact ccgcgagcag gctctctacg
    15901 gcgtcgaggt tggcctgtgc tacggtctcc accggggttc ccgcgttttc aacgcccagc
    15961 gtcacggccg ccgcttgcga cggattcttc cagatttctt cgttggcaaa cgccggaacc
    16021 aggaccttct cgaccgccgc tcgatcaaat tccagttttc tcttctcggc cagagcggcc
    16081 agaatgtccg ccatttcgga cgtccggtag gagaccggtg cgattgccgg atgaggagcg
    16141 ccgccgggct taatccagat cacgcggtcg gccgccacgc gttccaggac ccgcgccaaa
    16201 ccctgattgc cgccgtactg ctgcgacacg aaggccagag tgaccgcgtt cgagggcggc
    16261 gcttgcctca tcctttccgc aagggcgatc aaaactgctg ccccggcccg agccgagatc
    16321 cagggagcgc tcattcgctt accggacccg agctcggtat aggatttctc gagcgtgatt
    16381 gggtcaagca gatcgacgcc tgcttcccga gcctctcttt ccgagcgcgc cccgatatcc
    16441 acgtacatct gctccggatg ctccgtcttg gctgccgccg cgcgctccgt ttgcaggtgc
    16501 acggaaagac cggccaccac cccgttgatt gctcgcccgc tcgccgttgt gactctcacc
    16561 ggctgcgcca aaaagaagcg gtcgaaatcg gggtgcggtg gattgggcga caggcgatag
    16621 acccggaggt agccatcgga agtgatggct gacaccagat agccgggctc atcgagtccc
    16681 gcaataaaca acgtatgcgg ccggcctgcg ccgaatgcgg ccgtcagcga cccggtattg
    16741 tccacttcct gcctgccgcc cacggccttc cggacatagt cgaggacgga gccttcgtag
    16801 cccgaagcgc catcgatcgt ggctagcgct cgtagatcgt ctcccgcccg cagaaaaaaa
    16861 cagcaggcca ggcatatcgc cgccagacgg agccaggcga gccggcctcg tgtaacccga
    16921 tgcggcttgc cggcggcgcc gttgcaggcg aggcggctca ggtcaaccat gttttcaccg
    16981 ggcgcgtcct gtcccccaac ggatccaagc tggcgggcgc gcgggggccg gctatacttt
    17041 tagatataag caatacaatc aatttccacc agagaattgc cgggaatccc gccggccgca
    17101 gcgatcgtcg ttcgcacggg cggctcgggg ccgaaacgcc cgcggaacac ctcgttcatg
    17161 gcctggtagt cttttaagtc gttgaggtag acgttgacct tcaacacttt ttccatggac
    17221 gagccggcgt tcaccagagc tttttccatc tcgtccagca catgtttcgt gtgggccttg
    17281 atgtcgcctg cgaaatgggc cccgattcct gcgagaaata gaaggttgcc gtatgagacg
    17341 gcgccgctga aaagcggcgg ctgagccgct ttcgagccgg atggcctcgc ccggtcatgt
    17401 accttcttta ccggggcagt ttccgcggcg cgggctcgag cagctcccgc cagcgccgca
    17461 cccgccccgg ccgccttcaa aaagttccgg cgattgtttt ttgcgctcat ccgatcgctc
    17521 ctttttcgag tttggatgtt atcaagccgt ttctttcacc ggtccaggcc gaagaatgaa
    17581 tttcggccgg ccgtcctgat tcgccaactc ccaagccagc cgcatcgtaa gctgcgtgac
    17641 cttcaacatc ttcgtgaaat ccagccgatc aggagtatcg gtgacctggt gatattcggg
    17701 atgaaaaccg ccaaacagcc acacggcggg tatgtgctcg agagcgaatg gataatgatc
    17761 gcaccgctgc atgatgtcca gggagtgatc ggcatcgaga cggaaatcga gcttcagccc
    17821 cactctcccg ttagcggcct ccatagcccg ccggaggtcc ggcgaataat gggtcccaac
    17881 cgggtcgaga acgttggcag tgtcggccgg aatgtgccat cgcccgtcgg tctccggccc
    17941 aggcgtctca ttgcggccga tcatatcgag gtcgaggacg gcaacggttc cctccagcgg
    18001 ccgtaaggga tgccggacgt agtaaaagga accgagcagc ccctcctcct ccgagccgaa
    18061 cacaataaaa agaagcgacc gccgcggccg ggcgcccgag ccggcaaatg ctcgggccag
    18121 ttccagaacg gctgccgtgc cactggcgtt gtcgttagcg ccggggaagt attgcccgcc
    18181 ggccgctccc aagtggtcgt agtgagcgca cacgatcacc gtctcgcttt tcagcttcgg
    18241 gtcgctgccc tcgatccagg cggcgacatt tacggaagaa accgcccggg accgcagcaa
    18301 gcgatgattg agggtcatac ggatgccggc cagttcgaag gattgtgggc gcagagcagc
    18361 gtcgatcctg ctctcgagat ccgcccgctg tttgtgtgcg gcccgcaaca ggtcgtcggc
    18421 gacgtgagag gcgatgctca ccagagggat gccggtttct tcctcggcaa gggcttgcgc
    18481 cggaacattc ggttccatcg ccttgccgga gctggcgcga atgcgggcgc tgcttctgcc
    18541 ggcggcaagc aggagcccgg cggctccgtg tttcgcggcg ttgagcagct tggcgtggct
    18601 gcctgcgtaa agagtatcgc ccctgccgtt gaagacgctg tcgccgcgtt ccctctgcgg
    18661 ctctcctgca agcgccagca cgaactttcc acgggcgttc aggcccgcat agtcgtcata
    18721 atgcagctcg ggtgcggtga tgccataacc ggcgaacacg acgccggcct ccgtctcggc
    18781 gcttttcggg aaccggcaga aaaaattccc cggcggatga aataccgtgc tcaggccggg
    18841 ctgcttctgg aacagccatc ccagggcgga ttcgctgtca tcgatctcga attccagcag
    18901 agggatctgt tgaaaatagc tcccggcgcc ggccacgggt tggagtccgg cgtttcgcat
    18961 ggcagcggcg acgaaatccg ccgctgattg cgatcccggc tcgcccgaaa gcctgcccgc
    19021 gagcgcccgg gagcacagga atgtcacgtc cgagcgcatc cgttcggcgc tcaaccgtcc
    19081 taccgggacg tcgaccgaag gcgccgcgcc cgggaccgcg cccaacgcgt atgccaggag
    19141 cgcggcgaaa atccacgcaa tcgctgttgg cggccgcccg ttcatcgcga ccgcccgagt
    19201 tgatataggc cgccatccac gagcaacgct tctcccgtca tagcggttgc gtcggcggct
    19261 agaaacaggg ccgcttgggc cacttcctcg accgttgcca gccgccccag gggagtcact
    19321 ttggcgccgt gctcaaacgc ttcctcgggc cagcccgcaa atcgcgtacg taccaagccc
    19381 gggcacacgg cattcacccg aatatcggga gccagcacgc gcgccaacga cttggtcagt
    19441 gagagcgcgc ctgctttcgc cgccgcatag gccatggagc ttcccgcggc gtggaaggcc
    19501 gccgacgaca ccaggtttac gatggaagcc cccttttgct tccgcatcag cggcacggcc
    19561 gcccgcacgc agtagaacag gccgcgcaag ttgacgtcga acgtccggtc ccagatctcg
    19621 tctgtcagag cgtcgagctg gtggtgcggc actcgcgtac tccatcccgc gttgttcatc
    19681 agcacgtcca gccggcccca gcgtctctct acctcggcga tcatcccttt gacgtcggct
    19741 tcgcggcgca cgtcagcgcg gtatgcggct gcatttccgc cgttcgcgca gatgtcccgc
    19801 gcgacctcct cagcttctgc ctgcgattgg ctgtaattca cgacgaccgt ggcgccctcg
    19861 cgggcaaaca gatgcgagat ggcccggcca attcccgtcc cgccgcctgt gaccacggcg
    19921 atgcgatctt tgagtttcat gacgaaagta ccggctacgg acaaggcgcc cgcctgcctc
    19981 gatcgcttgc caggcccagc aggcggcgcg cccgcagaca tccacggccg cgggccaagg
    20041 ccggcaaggg gacgcggcaa gctatcctgc aattccagcg actacttcat agaacattcg
    20101 caggcccttt tcaaacgggg ccaccggcat ccgctcggca tcggaatgca tggacatcac
    20161 ttcttcggcc gacagcacta ccggaaaaaa tccgtagaca tagcggtccg ggcggcggaa
    20221 gaaattgcca tcggtgccaa aaggggtcag atacggcgtc accttggcgt tcggcgcttc
    20281 cctggccacg gtcgtttcga tcagccggaa gagctcggtg tccgccgggc ttggcggttg
    20341 agagggctgg tgatgtacat tctcgatttt caatttcggc tcggcgccag tcttgcgaac
    20401 ccactcgata aattcgtcga ccggttggcc tggcagcaaa cggcaatcga tcgaggcggt
    20461 cgcgatggac ggaaccacat tgtctttcgg cgggtcgccc acgcccgcgc ggaagctggt
    20521 gagcgcaatg gaatttcgcc gcatcgaatt ggcaaatcgg ttgtcagcca gagttcccag
    20581 ccgggtcagc atctggtcca aaatcgggtc ggtgcgtttg ggcggagccg tttcgacaat
    20641 gcggcggagc acgccggaga gcatagcaat ggcgttgtca tcggcaggct gggatccatg
    20701 tccgccggtg ccctccacgg tgaggtgcag ccatagcacc tgcttctgcg ccaccgagac
    20761 cccgtacaca tcgccttccg gcgtaaacag ccggttcgcc gagaagccgc cctcttcgag
    20821 tacgtactcc gactcgactt tctgaggata tttgtcgagc agaaatttcg cgcccgagcc
    20881 cccgccgact tcctcatcgg ctgtcgcagc aaagatgaca tcgcgcttga gagggacgcg
    20941 gttccgcttg agcagcagga acgccatcag gtggagaatg ccggtggact tcatatccac
    21001 agctccccgg ccccagagcc ggttgagctg tatttccgct ccccaaggat cggtctgcca
    21061 ccggctgcgg tcggcgggga cggtgtcggt gtggttcagg agcagcagcg cgcgcttgct
    21121 cccgttgcct ggcaggcgcg cgagcagatt cacttttccc ggcgccgctt ccagccgctc
    21181 actttggatg ccttcttttt gcagaactcc ggccagaaaa ctcaccgcgc ctgctacatt
    21241 gcccggtggg ttcgaagtgt caatgcggat atattcgcgc aagcggccga tggcctcttg
    21301 cgatgctttg gtccaatcgg ttcctgcccc tcgcgcggca ggcgcgccgg cggcggccgc
    21361 tgccatgccg agcaacagtt ttctccgcgt catgcgcact attctaaccc aggccctccg
    21421 ctaaaccgaa cggaacggcg acgctcggtg cgccgtctca ccgcacaccg gcgccggaat
    21481 tcaggagtct ctctttgcag agcgccggcc ggccaggttt ctattccgct ttttttgagc
    21541 gccagacgta ttgacaccca aatccccgtc cgcataatcg cgtggaaggg agcggcgcat
    21601 gttgcgtata aattcctggc gcggagccgg cgcggtgttg tggttgctta cgtttggcat
    21661 ttcctcccct gcccagcggc gaggacgttg ggagtacctg ggggaggcca acgtggacgg
    21721 cgcactggat cacgacaaca tcgtcgtaac gggcgcgcgt ggcaagtacc gggccatcca
    21781 gatacgggtc gaacgagcgc ccatcgagtt tgatcgggtg atcgtgcatt acggcaatgg
    21841 aagcgcagaa ccgattccga tccgccgagg gattcccgcc ggaggcccga cgcgcgcgat
    21901 cgaccttccg ggcgaccgcc gatcgagagc gtagaattct ggtacgccca cgctggcagg
    21961 atgcagaacg ccccagggtt cgcctctttg ggatgcggta gctggcggcc gttctgcgta
    22021 ttgttctctc tttgtcgtct ctggagcgca tcgctctgcg aaccccgccc ctccgcgttc
    22081 caaagtcggg cgtcgccaag atagatgccg atcgggcgat ggcaatttcc ctccgcgccg
    22141 aacggatcgg gattggctcg cccgagctca gtttgctcag cttttgcgag cgtcctactt
    22201 tttgcgttcc cacatccaga accctgccgg cttttccatc accgccggtt tcgtgtgtta
    22261 gcatgaaacc tggacttgac caggcgtccg gagaggtgct ggagtggtcg aacagggctg
    22321 cctgctaagc agttacactg gcaaaaccgg tgtcgggggt tcgaatcccc ccctctccgc
    22381 cagaatcttt ggctctacac caagagggta gctgaacccc aagagcagcg aaggacacac
    22441 gcgccatctt ctcggctgga gcttgattgg gccgcggagt ttagcgtttc agagaagaaa
    22501 taattgccga taagcctgcc gcgctctctc gaggttatat tcggcttcaa tttttttccg
    22561 gccggcctcc cccatttcgc gtcggcgccc ggcattgctg agcagtgcgt cgatgacttt
    22621 agcgaactct gcggcttcgc gccgccccgg ctcgacaagg accccattat gctcatcgac
    22681 aacctcatcg atcgcgccgg ttcgggaaca gatcacggga cgctggctcg cgagggcctc
    22741 gagaacaatg aggggaatgc cttctgcttg cgacggaacg acaacgagat cgcagccggc
    22801 caccaccgcg ggcatttcct cgacatgtcc cagcagcgag aagagcgctt cgatgccccc
    22861 cgtgcgaatt cgcgcctcga gtgcgctcct ctgctcgcca tcgccggcga ccacgaaatg
    22921 aaagtcgcgc gccggccgaa gccgcaccag ttcggctgca atatcggcca gaagcagtgg
    22981 gcgtttgatt gggtcaagcc tgccggcgaa caaaatgttc tttacggcgg cgccgggaaa
    23041 aggcgcaggc cggaatcgct cgagatccac gccgttccga atgagccaga ttttttccgc
    23101 cgctacacct gcggcgcgaa cgcgtctccg cgctgcatcc gagatgacga tgcgcaggtc
    23161 gatttcagag gcgacagggg cgctcgcact cacgaaatcc catgcgcggt ccaccgcgtg
    23221 gatccagtcc acaatctgca acgggggtag aagactgcgc aggtggggaa tggcgctgta
    23281 ggcggcgagc gagttctgaa taacgatagt gtgaaactgc cagttacgga ctatagaaca
    23341 aatcgccgca atcatacgat ctgatccgac gaagcgcgcc aggtcgtaga catgatcggt
    23401 cctttggcgc catcgctgct cccagcggga atcgtgcgat tgcgtggcga tcaagaagat
    23461 ctcgcaggca gcacggtcga tggctccggc cgcttccaac agcacgcttt ccgctcctcc
    23521 ggggccgagg tgcggcgtga gcagggcgat gcggcggcgg tccgccgggc gcggcatata
    23581 gcagagcggc tcgacaagct ggcctaaggc caagatcgat ttgggctgaa atttgagata
    23641 gaagctcagg tcaaatacgc tgcggcccgc cgcccggttg atgctctcct tgattcgcaa
    23701 agggatcagc cggccggccg attggagcgg atggcggagc caggagctaa gggacagcaa
    23761 ctcggcgttg accagatgcc ggcgcaccag ctcccagacc cttctcccgg cagccggacg
    23821 ggcggcgcag gggacgccgg cagattcgat tttttccgcg atttcccgaa aatcgttgat
    23881 cctttccagg gtccggcggc ttgcgcacag gcatccatcg ggcaggcgcg tagctgcgcg
    23941 cccgctccac acagccaggg cgcagagttc agcgctgtga gcgtcgattt ctccggccgg
    24001 cggaatcaga aaagccggag cgcgcgatcg ctcgagggcg gtgcccggaa gagatgccgg
    24061 ttgctcctgg acgtccttga tcgtttgcgc cgccgcgacg gcgcgtccca caatgcagac
    24121 cgccggcgcc cattgcgcct tgatgcgggc gcggccctcc ggcgaataaa gctgtgggtg
    24181 attcgcccgt atttttgcga ccagttctgc atggtgttcg cgcgccacag tcagcagcga
    24241 gcggccgtgt ttgcggtatt caaacagggc tcgcggcaaa tgacgcccat atcgcccgcg
    24301 ctccgccagc cgcagccaga agtcccagtc ctcgtaaccg aggcgcatgg aatcgtcata
    24361 accgccagcc gcttcccaat cgcttcgccg gatgagagaa gcgtagatca gcgtattctg
    24421 atcgagcagc cggaaaaggt tgtaatcgtc gagacgctcg acatactgtt tttcgccgaa
    24481 aacgcgatag tcggtgtaga taaaggcggc ctccggatga gcccgggcct cgcgaacgca
    24541 ttcgctgacg aacgcgggat ccagccggtc atcggcgtca agtggaacga cgtagtcgcc
    24601 gcgcgccgcg cgaaagccgc tgttgcgggc cgcggccaga ccctggttgc tctgctcgat
    24661 gtagcggtcg gccccgccgg ccgcggattt gagaagcttg cgcgcctccg gatgatccgt
    24721 gccatcatcg accagaatga tttcgacccg gccatacgtt tgggcgcgga cgctggctaa
    24781 tgtctctttc aaaaaacggg tggggttgta gcaaggaatg acaaccgaaa cagatcccat
    24841 taccgtgatg ccgccggcgg cagcggtata taaatgcacc gatccgcctc gctgaatcgg
    24901 agcgtgctcg agcggatggc aatccgctca tagttgcggg caagatcgca gagcccggcc
    24961 cgcaggaatc tggcctcagc gcttagtctt gatgcctccc gcagaatatc ctgcgaagcg
    25021 ctgttttcga gcatccggaa agcctgatca aaagacagca gaatcggtga atccgccgtt
    25081 tgctcggcca cggcgtggcc gaaaaagtat agcttttctt cttcggaacg gtggagcgtt
    25141 tcccactgca gccggagccg caaaacatct gcggcccggg cgaaagaata gccggtcagc
    25201 cgcactccgt tggcaaactc gacgccgcag cgctccattg gatgcggctc caggcggaac
    25261 cggtaatcag ccggcggctt ccggttcggc tcaaagaccg ccgccgtgta caaatcccgg
    25321 accgggaccg tcgaagccca gagagtggcc cgcatttggg cttcgggatc gaacaagccg
    25381 aaatgcaagc gagccccctc ggcgcaagat ggcgggagca ggaggcaacg cgactcatac
    25441 ccctcatcgc ccgggcgcca cgaggtcaga ttcggcctcc cacgcagaat ttcatgatcc
    25501 atagagccta aaagcgctcc ctgcgggttg acaatgtgtg cgaaacagcg ccagcgcctc
    25561 tcgggagtct tcaagcaccg ccagcgggtt tccagcacaa acacgccgca tttttccgag
    25621 aaacgcaatc cggtgacctg aagctgatct gcaaaatgca gcgccacgtc atcgatctgg
    25681 ggtagtggga ccggcgcaga ccccggcgaa aagcagtccg gcgccgcgct gagatcctcg
    25741 atgctcgttt cgccgagaag aagcgttttc ccggacgcgg gcgccttcgc agcggcagcg
    25801 atcatggcct cgagatccat ccacgcatcc gattccagca gccatcgccg gccggccttc
    25861 gaaatcacca gccgcagatc aggatccgct tcggcgagaa aggcgacgcc ctcgggagct
    25921 tccgcgctct cgtgaatcaa ctcgatcacc cggactgcgg gcagccgctc ccggaggaaa
    25981 ggaacaatgc tgagacccgg ggggcaattc tggattacaa gcacgtcaat tttccagttg
    26041 accacgaacc aataatacgc ggccgtcatc tctgccggct cgatcagcgg ggccagattg
    26101 tagacgtgtg gagtgatctg ccgccaccgg tcgcgccatt ccgagcgatt cgcttcgagc
    26161 gcaatgatga acaggtcgca gcggtcgcga tccacgctgt ccgctagctt gagcagcaga
    26221 ttgtgttgtt cgcccggccc cagatggggg acgatcaggg ccacccttcg ctttgaggac
    26281 ccgtccctcg ccggcggcgt cggccgcgtc aaataacaca gcgactctgg ccggggaacc
    26341 tgtcgtgttg gagcgtgttg ctcgatggcg gcaagacttg caactccgct caacatgccg
    26401 cgagcgagac ccagggagcc gtccgggagg cgcacgaccg atttgccgct gaaaacagcc
    26461 agtgcgcaca gttccgcgct ttgcggatcg gtgattccct cgagcgccgg aatcaggaac
    26521 gcgggagccc gcgaagccgc caatgcgcga gccggatctg tttcgcagag aacttggaca
    26581 tcctcgatgg tttgccgggc gggcactagg ccctcctccg cggccagaat acatacagcc
    26641 ggctcccagc agatcttagc ctgtaggatt ccctcgcgcg aatacagccg cgggtgattg
    26701 gcgcggatcc tcgcgacgag ttcttcatgc cgctcccgag ccgcgctgta caaagaaggg
    26761 ccgtgctttc tgtattcata cagcactttc ggcacatagc ggccgtagcg gccctgctca
    26821 gcgagccgga gccaaaagtc ccagtcctcg taaccgtcgc gcatcgattc gtcgtatccg
    26881 ccggccagtt tccagtctgc tttcctcacc aaagaggcgg cgctgacggt gttgtggtcg
    26941 agcaactgat agagattgaa gtcagcggcc cgctggatgt actccacgtc gccgaaaact
    27001 tgatattccg aataggcata agcggcctcg gggtgcgctt cgagcagggg cacgcattcg
    27061 gccagataat ttggcttgag gcggtcgtcg gaatcgagcg gcaacacata cgtcccggcg
    27121 gccgctcgaa agccggcgtt ccgcgcggct gatacgccgc ggttcgtttg gcgaatgaag
    27181 cggtccactc gcgggcgcgc cagttcaaga tattggcagg ctgccagccg gtcggtcccg
    27241 tcgtccacga ggatgatctc caggggcgaa tatgtttgcg cgcggacgct gtcgatggct
    27301 tcctcgagga agcggctcgg gttgtagcag gggatgacaa cggaaaccag cgggggaaac
    27361 ggcatagcct agggagagga gcgcggcggg agcggaagaa aaatcgcttt ctgctgcttg
    27421 ctcatcgtca ggctgcttga tcggatcgca aggcggtcca gcgggcgatc gatgtcgaat
    27481 agccctgccc gtaaaaattt ggcttgatcg ctgagtttcg aaacatcgcg gacgatgtcc
    27541 tggatcaggg tgaattgccc gtcacgggac cgaaccgtcc ggttcaattc cgtgtcttcg
    27601 tcgaaggaca gcagaatggg cgcgccagcg gactggtcag caaccgcatg gccgaaaaaa
    27661 tagagcttag ccttaggccg cctccccgcc gaccagcgca accggagcca ggccacgccg
    27721 ccctggcgcc gcagagagta gcctgtgatc tggatcccgc cttcgaactc tacattgcag
    27781 gctatgagag gagccggacc catttggaac tggtattcgg cggcaggggg ctgattggga
    27841 agaaacacgg ccgcagtgta atcgtccttc agaggagcag tcgaggccca caggggaacg
    27901 cgccgtcccg attcgggatc aaacaatcct agcctcacct gtgcgcctct ggccgtggcg
    27961 ggcggcagcg caagataccg ggcttcgtag ccttcgtcgc cgggctgcca ttcggttacc
    28021 ggcgggctgc cgtggagaat ctcgtgatcc atcgagctca gcagcttgcc ttcggaatcg
    28081 acgacatggg cgaagcaacg cagaggcatc gggggcggcg tcaggcaccg ccagcgcagc
    28141 gccagggtaa aaatgccatc gcgctctgtg aggcgcagcc cggcgacttg cagccaatt
//
LOCUS       DASREK010000308         8420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_973471, whole genome shotgun sequence.
ACCESSION   DASREK010000308 DASREK010000000
VERSION     DASREK010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973471"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2761)
                     /locus_tag="VFA54_16620"
     CDS             complement(<1..2761)
                     /locus_tag="VFA54_16620"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HZS52495.1"
                     /translation="MNQIEEGLPVIDWPVGRARRMRQQRRLCILLVAAALSSAALAMA
                     TDSLAREYSRRVWRTEDGLPQNRIQALAQTPDGYLWIGTSEGLARFDGLRFVSFYRSN
                     TPAFQDDSILALLAARDGSLWIGTEGGGLLHYRNGTFRALGVPEGLTNGFVRALYEDG
                     HGSLWVGTDRGLFRLAAERFERLDGTPEVPLATVVSITADQIGNLWVATSSGLLTMEG
                     EKLVPIRCSRGPLLSRHALFAAHDGALYIATHSGAQRIRGGCMVPDTTLPAFPLILVR
                     EDHERNLWLGTIGHGLIRSRAGVLARFEGLDILAGNTVTAFLEDQEYNLWIGGQDGLL
                     RLSKTAVATIGARDGLADDNISTVYEDRSQNLWITTATGQIYRLNKGKSAPERVQLPA
                     PVTSAQVRNVFEDSRGAYWFGTQGSGVIRWWRGQVATYSKKDGLRGDIVRQIYEDHAG
                     VIWLATSSGLSRREADGFRSYYLEDGLTYPSVRCIASSRSGDLLVGTDGGLNIVHDGK
                     ILPNPIAGLGREKIWSIYEDRGGGLWLGTRGGGLVRIKLGKIARLSMRDGLPSNSIYQ
                     IVEGHPGKLWMSSPAGVFSARQDDLDEAADGKSSALHVVLYGASEGMETSQMSGGTQP
                     AGCKASSGEIWFASVRGVVRVNPASELPGRSAPVLIERVLADDRVVPLGREVVTPHGH
                     GTVQIDFTACNLAAPQRESFRYKLEGFDERWTSPAKSRSAYYTNLPPGRYRFRVEASD
                     TGAPGNATEASFILVSRAPFYQTVWFYTLCALALSMLVWFGLWLNARQARARYAVLLA
                     ERTRLAREMHDTVIQGCVGISALLEAAVKCQRSNLEEAFNLLDHAREQVQSTLEEARQ
                     AVWNLRHDLGHESAISKLRGLAQKLGRENGLLTHTEVAGKRAPLDPQTDRTLLLVGRE
                     ALRNAV"
     gene            complement(2758..4866)
                     /locus_tag="VFA54_16625"
     CDS             complement(2758..4866)
                     /locus_tag="VFA54_16625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52496.1"
                     /translation="MFIRDGAAFLRRHQVGFDGGVSNVVEHRIDYWFGSGDHARSYVS
                     RTVAGDLIELPVTWYAEQGGRWGMSPGYDWAGHPGFSRKITYRCMFCHNAYPETAPSD
                     DLWDRATRWPRRLPEGIDCQRCHGPGRAHIEAAQQGRPAALVRAAIVNPARLTARRQM
                     EICLQCHLETTNTNLPAALLRYGRGVFSYRPGEPLGDYMIYFDHAPGSGHDDKFEFTG
                     APYRLRKSRCYQESHEALICTTCHDPHRALSSTEMAARSSRACQRCHAAGIAALVRSQ
                     RHREREDCVSCHMPKRRPTDAVHVTITDHWIQRSPQPAGMPAESSGADRPYRGKVVLY
                     YPETLAPNSDADLYLSIAQVRNQANLKDGLDDLRQAVDQWRPAQAGFYSDLGDAYRHS
                     GRLQEAIDAYRKACSLDPNWWPARLGLGLSLAATGDTQHALAELNRAETAAPWETEIV
                     KFQAATLAESGNPEAAVAKLRSAVAADPDSGDLHNSLGAALTRSGDIQGAEAALREAV
                     RLRPEIAPVHVNLATVLARTNKFREARYEFREALRIDGSSAEAHSAYGAALAAHNDFR
                     EAREQFEIALRLNPGLWNTYNNLGSVLNQLGDKTAAAAAFRAAIAIRPDFPTAQYNLG
                     ALLADSGNFAEAEQHLREAIRGAPHYYEAHLKLGGLLCEMQRCQEGAAHLLAAAQSPD
                     PALRQAAQNTLSRAAAAAQQ"
     gene            complement(5493..6665)
                     /locus_tag="VFA54_16630"
     CDS             complement(5493..6665)
                     /locus_tag="VFA54_16630"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52497.1"
                     /translation="MADITEIAAETYRISIYVPEIDLQFNHFLVRDEQPLLFHAGLRR
                     LFPEVHEAVRTLLDPARIRYISFSHFESDECGALNEWLAQAPQAEPVSGLVGAVVNLN
                     DFASRPARALSREETLHTGRHRFRFISTPQVPHGWDAGVLFDETERILFCSDLFHQWG
                     DVEALTESSVLERVQEALRRIESGPLARYVPYTHHTGGILEDLSRLDPRTLAIQHGSS
                     FRGNGAAALRDLAEIWRTTLGPPAREQVAGPASRQQQRFAGTLYERLGGYDTIAAIVD
                     DLLAMLREDPRFGRFVMERSLDSHQRARQLLVDQLCALAGGPCFYVGRDMKTSHAGLG
                     ITESEWNANLEYAIAALKKNGVPQAEQGEFLSLFTRYREEIVERSPAQARDQPLDR"
     gene            6927..7211
                     /locus_tag="VFA54_16635"
     CDS             6927..7211
                     /locus_tag="VFA54_16635"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HZS52498.1"
                     /translation="MRVESYYLHTAPRTAKIAMSLPVDLYRALERIRKTTGQPRSALI
                     RQALRYWLERQREEALARRYESGYRLHPEDSDEIRRAEAAAVELLASQEW"
     gene            complement(7642..7731)
                     /locus_tag="VFA54_16640"
     tRNA            complement(7642..7731)
                     /locus_tag="VFA54_16640"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7695..7697),aa:Ser,seq:tga)
     gene            complement(7967..>8420)
                     /locus_tag="VFA54_16645"
     CDS             complement(7967..>8420)
                     /locus_tag="VFA54_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HZS52499.1"
                     /translation="RKLIFCSSNVEGWAHYCEQMMLDEGFGGGDPKIRLAQLEEALLR
                     DCRYVVGIKLHTQGMTVDEGARFFVEKGFQEPANAYEEARRGAYNPTYLYYTLGKLEI
                     LKLREDYHKAKGPAYSLESFHNEFIKQGGIPIRLIRRILLPGDNSPVL"
BASE COUNT         1601 a         2625 c         2574 g         1620 t
ORIGIN      
        1 ccactgcatt ccgcagagcc tcgcggccga ccagaagcaa ggtccgatcg gtctgcggat
       61 caagcggcgc ccgtttccca gccacttcgg tatgcgtgag caggccgttt tcccgcccca
      121 gcttttgagc cagtccgcgc agtttggaaa tcgccgactc gtgacccaag tcgtgccgga
      181 ggttccagac ggcttgacgc gcctcttcga gagtggactg cacctgttcg cgcgcatgat
      241 ccaaaaggtt gaaggcttcc tccaggttcg accgctggca cttgacagcc gcttccaaca
      301 gcgctgatat gccgacacag ccctggatca ccgtgtcgtg catctcgcgg gccaaacggg
      361 ttcgttctgc aagcaaaacg gcataacgcg ctctcgcttg gcgcgcgttt aaccacaatc
      421 caaaccacac cagcatcgat agagccagtg cgcacagggt atagaaccag actgtttgat
      481 agaacggagc ccgggaaacc aagatgaaag aggcctcggt ggcatttccc ggggcgcctg
      541 tgtcgctggc ctccacgcga aaacggtatc gccctggagg aagattggtg taataagccg
      601 accggctctt cgccggtgat gtccatctct cgtcgaagcc ctccagtttg taccgaaagc
      661 tttcgcgctg cggcgccgcc aggttgcagg ccgtaaagtc gatttgaacc gtgccgtgac
      721 cgtgcggagt gacgacttca cggcccagcg ggactactcg gtcgtccgcc aggactcgct
      781 cgatcaatac gggagcggaa cggcccggca actcgctggc cgggttaacg cgcaccactc
      841 cacgtacgct ggcgaaccag atctcgccgg atgaagcctt gcagcccgcc ggctgagttc
      901 cgccgctcat ctggctggtt tccattccct cggacgcgcc gtacaacacc acatggagag
      961 cgctggactt gccatcggcc gcctcatcca gatcatcctg gcgggccgag aagacgcccg
     1021 cagggctgct catccagagc ttgccaggat gcccttcaac gatctgataa atggaattgc
     1081 tgggcagccc gtcccgcatc gaaagtctgg caatttttcc gagcttgatg cggaccaagc
     1141 cgccgcctcg agttccgagc cacaggccgc ctccgcgatc ttcgtaaatc gaccagatct
     1201 tttcccggcc cagcccggcg attggattgg gcaggatttt tccatcgtgc acaatgttga
     1261 gcccgccgtc cgtccccacc agcaggtcgc cgctgcggct ggaggcgatg catcgcacgc
     1321 tcggatatgt cagaccatct tcgaggtagt agctccgaaa accatccgcc tcccggcggc
     1381 tcaacccgct gctggttgcc aaccagatta cgccggcatg gtcttcatag atctggcgaa
     1441 cgatgtcgcc cctgaggccg tcctttttcg aataagtggc gacttgcccg cgccaccacc
     1501 gaattacccc cgaaccctgg gtgccgaacc aataagcgcc tctactgtcc tcgaacacat
     1561 tgcggacctg ggcggaggtg acaggggccg ggagctggac gcgctcggga gcgcttttac
     1621 ctttgttcag gcgatagatc tggccagtcg ccgtggtgat ccacaaattc tggctgcggt
     1681 cttcatagac ggtggagatg ttgtcgtccg ccaggccgtc gcgagcgcct atggttgcca
     1741 cggccgtctt gctgaggcga agcaacccat cctgtccgcc aatccagaga ttgtattctt
     1801 ggtcttccag gaatgccgtc acggtattgc cggccagaat atccagtccc tcgaaccgcg
     1861 caagcacacc ggcccgggag cggatcaagc cgtgcccgat ggtccctagc cataggtttc
     1921 gctcgtggtc ttcacggacg agtattaggg ggaaggccgg cagcgtcgta tccggaacca
     1981 tgcagccacc gcggatgcgc tgagcgccgg aatgagtggc gatgtacagg gcgccgtcat
     2041 gagctgcaaa taaagcgtgt cttgacaaca gcggcccgcg gctacagcgg ataggtacta
     2101 acttttcccc ttccatggtg agcagaccgc ttgaggtggc gacccaaagg tttccgattt
     2161 ggtcggccgt tatcgacacc accgtcgcta acggaacctc cggtgttccg tcgaggcgct
     2221 cgaagcgctc tgctgccagc cggaacagcc cgcggtccgt tccgacccac agtgatccat
     2281 gaccgtcttc ataaagggcg cggacgaaac cgtttgtcag gccctccggg acgccaaggg
     2341 ctcgaaatgt cccgttgcgg tagtgcagca aacctcctcc ctctgtgccg atccaaaggg
     2401 agccgtcccg cgccgccagt aaggcgagta tgctgtcgtc ctgaaatgcc ggagtgttgg
     2461 accggtagaa ggatacgaat cgcaggccgt caaatcgagc caggccttcc gaggtgccga
     2521 tccagagata accatcgggg gtctgtgcca gcgcttgtat ccggttctgt ggcaatccgt
     2581 cttccgtacg ccagacgcgg cggctatatt cgcgcgccaa cgagtcggtg gccatagcga
     2641 gagcggcgga tgagagagcc gcggcaacca gcagaatgca aagccgacgc tgctgccgca
     2701 tccggcgtgc tctcccaacc ggccaatcga tcacgggcag gccctcctcg atttggttca
     2761 ctgctgcgca gcggcggccg cccgactcag cgtgttttgc gcggcctgcc gcaaagctgg
     2821 atccggactc tgagccgccg ccagcaggtg ggcggctccc tcctggcaac gctgcatctc
     2881 gcacagcagg ccgccaagct tcagatgcgc ttcgtagtaa tgcggagcgc cccggatggc
     2941 ttcacgcagg tgttgctcgg cctctgcgaa attgcctgaa tccgccaaca gtgcacccag
     3001 attgtattgc gcagttggaa aatcggggcg gatcgctatc gccgcgcgga atgcggcggc
     3061 ggctgccgtc ttgtcgccaa gctggttgag caccgatccc aggttgttgt atgtgttcca
     3121 caagcctggg tttaagcgca gcgcgatttc aaattgttcc cgcgcttccc ggaaatcgtt
     3181 atgggcagcg agggctgcac cgtaggcgga atgagcttcg gcggacgagc catcgatgcg
     3241 aagcgcttcc cgaaactcgt aacgcgcctc ccggaacttg ttcgtgcgcg ccagcactgt
     3301 cgccagattg acatgcaccg gtgcgatctc cgggcgaagc cggacggctt cgcgcagcgc
     3361 tgcttcggcc ccctgaatat cgccgctccg tgttaatgcc gcgccgagac tgttgtgcag
     3421 atcgcccgag tcggggtccg ctgccacagc ggaacgcagc ttcgctactg ccgcttcggg
     3481 gttgccggac tccgccaggg ttgcggcctg gaatttgaca atctcggttt cccaaggcgc
     3541 ggcggtttca gcgcgattca gttcggccag ggcgtgctgc gtatctccgg tggcagccag
     3601 cgaaagcccg aggcccaagc gcgccggcca ccagttgggg tccagcgaac aagcctttct
     3661 gtaagcatcg atcgcttctt gcagtcggcc tgaatggcga taggcgtctc ccagatccga
     3721 gtagaaaccg gcttgcgcag gacgccactg gtccaccgcc tggcgtagat catccagtcc
     3781 gtctttcaag ttcgcctgat tgcggacctg cgcaatggag aggtagaggt cggcgtcgga
     3841 gtttggcgcc agtgtctccg ggtaatacag caccaccttg cctcggtagg gccgatcagc
     3901 gccggaggat tcggccggca tcccggcagg ttgtggagag cgttggatcc aatgatctgt
     3961 gatggttaca tggacagcgt cggttggccg cctcttgggc atgtggcacg atacgcagtc
     4021 ttcccgttcc cggtggcgct ggctgcgaac cagagccgcg attccggccg catgacacct
     4081 ttgacatgcg cgagaggatc gcgccgccat ttcggtggaa ctcagagccc ggtgagggtc
     4141 gtgacacgtg gtacaaatca gcgcttcgtg gctctcctga tagcaccgcg acttgcgaag
     4201 gcgataaggg gcgccggtga actcgaactt gtcatcgtga ccgctgcccg gcgcatgatc
     4261 aaaatagatc atgtaatcgc cgagcggctc gccgggacgg tacgaaaaca cgccgcgtcc
     4321 gtatcggaga agagcggcgg gcagattcgt gttggtggtc tccaaatgac actgtaggca
     4381 aatctccatt tgccggcgcg cggtcagccg cgcaggattc acgattgcgg cgcgaaccag
     4441 cgccgccggg cgtccttgct gagccgcctc aatatgcgct ctcccaggcc cgtgacagcg
     4501 ctggcaatcg attccctccg gaagccggcg cggccagcga gtcgcccgat cccagaggtc
     4561 gtcagatggg gccgtctccg gatacgcgtt gtggcagaac atgcacctgt aagtaatttt
     4621 gcggctgaat ccggggtgcc cggcccaatc gtagccgggg ctcattcccc agcgcccgcc
     4681 ttgctccgca taccacgtta caggcagctc aatgagatcg cccgcaaccg tgcggcttac
     4741 gtaactgcgc gcgtgatctc cggagccaaa ccaatagtcg atcctgtgct cgacaacatt
     4801 ggagacgcct ccgtcgaagc ccacttgatg gcgccggaga aaggcggctc catcacggat
     4861 gaacatggaa aaagattgcc gtgaagcttt atgttgaaat tcgccacgcc tgagctccgg
     4921 gaatgggctg gccgtcttca ctgctccgaa ggtccttgcc attccggtcc ggccgtagct
     4981 ttcggcaatg gtcttatgac accgggcgca ggcttccggg gcaacataat ccaaatccaa
     5041 agccggggct gagcaggcga cggaggccag gagcaataag cgtccaaatg gggttttcat
     5101 tgacgaacgc caggctcgta ttcggcggcc ggggccggtc tcaaaaacaa gcctacccat
     5161 catactcccg aggcaaacac gagcagacca taccacttgc cggggtgttc tttccagcct
     5221 ctgtcagaac ccagaacagg gcgccagaaa cgcgaatatc caaagtgccg gccagccgtt
     5281 catgcgattg aacctttgaa aaccgcagcg tgcagcgccg cgacgcagcg gttcgggtct
     5341 ggatattaca tgccagcgtt tcactcggcg ccgacagccg actgccgccg gaaggcggcg
     5401 agcaagcgcg gcttgcggag aaagatgccg cgaggtggct agcccgcgct ctcgcggatg
     5461 gcgggtgacg gccggcagcc caaacggtcg agctagcggt cgaggggctg atcccgggct
     5521 tgggccggcg agcgttcgac gatctcctcg cgatagcgtg taaacagcga caggaattcc
     5581 ccttgctcgg cttgcggcac gccgttcttt ttcagcgcgg cgatcgcgta ttccagattg
     5641 gcgttccact cgctctcagt gatccccagg ccggcgtgag acgttttcat gtcccggccg
     5701 acatagaagc aggggcctcc tgctaaagcg catagctggt ccacgagcaa ctgtcttgcc
     5761 cgctgatgag aatcgaggct gcgctccatg acgaaccggc caaagcgcgg gtcctcccga
     5821 agcatcgcca acaggtcgtc gacaatcgcg gcgatagtgt cgtatccgcc caggcgctcg
     5881 tacagtgttc cggcgaacct ctgctgttgc ctgctcgccg gaccggccac ttgttctcgg
     5941 gcgggcggac caagcgtggt tctccagatt tcggccagat cgcggagcgc cgctgcgccg
     6001 ttgccgcgaa agctcgatcc gtgctggatc gccagagtcc gtggatcgag ccgggacagg
     6061 tcctccagga ttccccccgt gtggtgagtg taaggcacgt accgggcgag aggtccggat
     6121 tcgatgcgcc tcagcgcctc ctgaacccgc tccagaaccg atgattcggt cagcgcctcc
     6181 acgtcgcccc attgatgaaa caggtcggag caaaacagaa tgcgctctgt ctcgtcgaag
     6241 agcactccgg cgtcccagcc atgaggcacc tgcggagtgg atatgaaacg gaaacggtgg
     6301 cgtccggtat ggagggtctc ctctctggac agggcgcgcg ccggcctgct ggcaaaatcg
     6361 ttcagattca ccaccgcgcc tacgaggccg ctgacgggct cagcctgcgg cgcctgcgcc
     6421 agccactcat tcaacgctcc acattcgtcg ctctcgaagt ggctaaagct gatgtatcgg
     6481 atccgcgccg gatcgagcaa cgtccggacc gcttcgtgga cctcggggaa cagccgtctc
     6541 aagccggcgt ggaatagcag cggctgctcg tcccgaacca ggaaatggtt gaactgtaga
     6601 tcgatttctg gtacgtaaat cgagatgcga taggtttcgg ccgcgatctc cgtaatatcc
     6661 gccatccccg ttttcctcct gcctgttgca attatgggca tcccgagcgg ccgggcgcaa
     6721 cagtttgccg cgagtctctc ttgccatcaa ccagcttccg tgtctgcacg taaaaattct
     6781 ggggtgagca gttcgggatt ggcggggcgt cgtggacact tttcgaactc tggcggtccc
     6841 cacaagctct gcttgtgcaa aggcaagccg cctccagcag gcgacttgca tttcgatcgg
     6901 cagcgattgg gggaggttga ctgtgggtgc gagtcgagtc atactatttg cataccgcgc
     6961 ctagaaccgc caagatcgcc atgagcctcc ccgtggatct ttacagggcc ttggagcgga
     7021 ttcgcaagac cactgggcag ccgcgaagcg ccctcatccg gcaggccttg cgatattggc
     7081 tggagcggca gcgggaagag gccctggccc gcaggtatga atccggctac cggcttcacc
     7141 cggaggattc tgatgagatc cgcagggcgg aggccgccgc tgtcgaactg ctggcatctc
     7201 aggagtggtg attgcggcgc ggcgaggtgt ggtgggcgaa gctgccccaa cccgccgggc
     7261 gtaggctgct cctgctgctt tcgcgtaaga ggcttacaca gttcgggacc tggtcacggt
     7321 agcgccgctg accacgcgca ttcggggcat ccccaccgag gtcccgctcg gggcagagga
     7381 tgggctgccg cggaggtgcg tcgttaacct ggatacggtt acgacgatcc ctaaaggaag
     7441 cctcgcggat cggatcactg ttcttagcac tgaaaaactg gtgaccgtcg agcaagcgct
     7501 ttgctttgcc ctcggcttga agctcggttg acctgaatcg agctcaccaa cctgctgaaa
     7561 acacgaaggg aggcgacaga atcagttaac tgcctgggag aaggctatgc cgaaccggct
     7621 gaaatgaaag tgccgaatct ctggcggagg aagagggatt cgaacccccg agtgagtttc
     7681 cccactaacg gttttcaaga ccgccgcctt caaccgctcg gccattcctc caaaaatagg
     7741 ctaccgcatt ttcggcccgg tgcgggccgt atccgcaggc atgggcgctt ttccatcgat
     7801 tgcaccgacc gccagaatgc agaaagggaa aagggatccc cggcggctca ccccgggtcc
     7861 gcgcaatgtt tgtcccgcct tacggagcgg gcgtcgcgct gccctcgatg cgcagccgcc
     7921 acaggccagc ggacaaaggg ctcaggccgg cgggcgctgg gccggctcac agcaccggcg
     7981 agttgtcgcc gggcagaagg atgcgccgga tcagccgaat cggaattcct ccctgtttga
     8041 tgaattcgtt gtgaaacgac tcgaggctgt aggccgggcc tttcgccttg tggtagtcct
     8101 cgcggagctt gagaatttcc agctttccca gagtgtagta caagtaggtt ggattgtaag
     8161 cgccgcgccg ggcctcttcg taggcgttcg ccggttcctg aaatcccttt tccacaaaaa
     8221 acctggcgcc ttcgtccacg gtcatgccct gcgtatggag cttgatgccg acgacgtagc
     8281 ggcaatcgcg caggagcgcc tcttcgagct gcgccaggcg gatcttggga tcgccgccgc
     8341 cgaagccctc atcgagcatc atctgctcgc agtaatgcgc ccacccttcc acgttgctgc
     8401 tgcaaaaaat cagcttcctg
//
LOCUS       DASREK010000309         6803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1307790, whole genome shotgun sequence.
ACCESSION   DASREK010000309 DASREK010000000
VERSION     DASREK010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307790"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFA54_16650"
     CDS             <1..224
                     /locus_tag="VFA54_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS52500.1"
                     /translation="QRSGAEDSYELLTPREREILQLIAEGKSNKEIAYQLNLSLYTVE
                     THRAHIMQKLNLQSVPELVLYAVRRGIIS"
     gene            complement(296..934)
                     /locus_tag="VFA54_16655"
     CDS             complement(296..934)
                     /locus_tag="VFA54_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52501.1"
                     /translation="MDLRDRSAARQSGRPIVCVYLTSSLWAATEAFHILTPANIRLCR
                     AVTLREARLLLRILPSRVLLVDTIFDDGQWADALEAVQREQLPVCVVVATAAYESDLW
                     AAILRAGGFDVVLKPFSRLTLVHAVQDAHRHACGFDEDRKGRPALEEVGPAEEKQASG
                     AIKGTSTAPAQARRRCRLNPTGLCRWLKRAFRGADGASTTPGAAKRVRRRPA"
     gene            1229..2707
                     /locus_tag="VFA54_16660"
     CDS             1229..2707
                     /locus_tag="VFA54_16660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HZS52502.1"
                     /translation="MSNVWTRSRRAFALLITSAALGALVVAGAVVHSSTGANPPASFQ
                     FADPHEGPSRNSFAPVVKKTLPAVVNISTSKIVKTPTGFSGEMPMDPFFRRFFGDEFG
                     NSFNAPRERRERSLGSGVIVSREGYILTNNHVIDGATDIRVTLSDKRELKARLIGADA
                     KTDIAVLKVDAANLPFMVLGDSSKAQVGDFVLAIGNPFGLGGTVTMGIISATGRGNLG
                     IEDYEDFIQTDAPINPGNSGGALVNDRGELIGINTAILSRSGGNQGVGFAVPINLART
                     VTDQILKTGHVTRGYLGIMLQPVTPAIAKAFGLKEAQGALVADVEPNSPAQRSGVEKG
                     DIILAVNGKAVTDVNQLRLSISMMAPGSAVDLKVFRNGADREIRAELGEMPSKQERAS
                     LENNGSGGALDGVDVQDLTPETAGALHLPPQTRGVVVTRIDPASEAAGSGLQEGDVIQ
                     EVNHQPVRNSTDFNRALRQSAQAGKPSLLLVDRQGTTLFIAV"
     gene            2828..3025
                     /locus_tag="VFA54_16665"
     CDS             2828..3025
                     /locus_tag="VFA54_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52503.1"
                     /translation="MHEAYVSARRRYAEELAKTITLLRLGEFADAFVGQRAREMEAFE
                     RFRIARQVYLHYIQDKEREAA"
     gene            3206..3460
                     /locus_tag="VFA54_16670"
     CDS             3206..3460
                     /locus_tag="VFA54_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52504.1"
                     /translation="MNPSDSLPAELIQKVLAQAPPGSWIIARRSDGKILGIGKTGSQE
                     ALQRAQADHPDLRLDEVVVLWKSAPGKEPETPPQSRSKAG"
     gene            4090..4272
                     /locus_tag="VFA54_16675"
     CDS             4090..4272
                     /locus_tag="VFA54_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52505.1"
                     /translation="MKFAWEAGEPVFGTQGTASIENGTTVVRAKLDLHRLAPGQYFFG
                     IRQPPWEWTYVPIVIR"
     gene            complement(4338..5999)
                     /gene="merA"
                     /locus_tag="VFA54_16680"
     CDS             complement(4338..5999)
                     /gene="merA"
                     /locus_tag="VFA54_16680"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HZS52506.1"
                     /translation="MANQAQFTLRIEGMTCENCARHVTEALRSVPGVTDVRVGSWKSG
                     QAAVVAGQEVDSKSLEAAVEKAGYHAVATEKRPVGNGRRVPSSGESFDWMTIGGGSAA
                     FAAAIKAAELGARVAIIEKGTIGGTCVNVGCVPSKTLIKAAELCYHSAYPNFQGLTAC
                     PPPSDWRRVVEQKDELVAMLRRDKYVHVAEQYANITILKGEARFTGGRSLEVNGHRYT
                     PGKILIASGSHPWAPPIPWLAEIPYLDSTAALSLPELPESMIVIGAGSVGLELAQLFG
                     RFGVRVMVFEAQPHLAAASEPEIGTALTQYLEGEKIRACVNAVIKRIDRKRDQYEVHA
                     RLDGRPEVCTAKQLLVATGRRPNTEGFGLETAGVQTGGRGEILVNEYLQTTNPDIYAA
                     GDCIGDPMYVHVAAYAGGLAAENALTGAGRLYDLSALPSVTFTDPQMASVGLTESQAR
                     AQGLKIKTAVLSLKDVPRAQASRDTRGLIKLVAEQATGRLLGAHALAADAGEVIQEAT
                     LAIRFGLTFEDITHTFHPYLTMVEGIKLAALTFEKDVAKLSCCAV"
     gene            complement(6011..6400)
                     /locus_tag="VFA54_16685"
     CDS             complement(6011..6400)
                     /locus_tag="VFA54_16685"
                     /inference="COORDINATES: protein motif:HMM:NF015180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HZS52507.1"
                     /translation="MSLRDHLDKVGIAGSFVAAACCMGLPAIVSILAALGLGFLINDA
                     VLRPLMIAFLAVAFIGLLFGFRVHRRLWALVIAAGKRRDCVSLHSYAPQRGASVSWDR
                     GTDPRQRSQRAYAPAVRSGVQALINIA"
BASE COUNT         1462 a         2074 c         2043 g         1224 t
ORIGIN      
        1 tgcaaagaag cggcgccgag gacagctatg agctgctcac tccgagagag cgcgaaatcc
       61 tgcaattgat cgccgagggg aaatcgaaca aggaaatcgc ctatcagcta aacctgagcc
      121 tctacaccgt agaaacgcac cgcgcgcaca tcatgcagaa gctgaatttg cagagcgttc
      181 cggaacttgt gctctacgcg gtacgcaggg gcatcatttc ctaaaaccaa gcccggctgt
      241 ccctcgagga ggctccggat aaccggagat ccaaccggca gccgacagcg gaggctcagg
      301 ccggccggcg tcgaactcgt ttcgccgcgc cgggcgtcgt gctcgcccca tcggcgcctc
      361 gaaaggcccg tttcaaccag cggcacaatc ccgtagggtt cagacggcat cgtcttctcg
      421 cctgagccgg cgccgtcgaa gttcccttga tcgctccact ggcctgtttc tcttctgccg
      481 ggccgacctc ttcgagcgcg ggacgtccct tcctgtcttc gtcaaagccg cacgcgtgcc
      541 ggtgagcgtc ctggacggca tgcacgagcg tgagccggga aaatggtttc agaaccacgt
      601 caaatccgcc agcccggaga attgcggccc agagatccga ctcataagcg gctgtcgcaa
      661 ccacgacgca caccggaagc tgctcccgct gcaccgcttc taacgcatcc gcccactgtc
      721 cgtcatcgaa aatggtgtct accagcagaa cccgcgacgg caagatgcgc agcagcaaac
      781 gcgcttcccg cagcgttacc gcccggcaaa gccggatatt agcgggggtc aagatatgga
      841 aagcctctgt ggcggcccaa agcgagctgg tcagatagac gcagacaatg ggccgtccgc
      901 tctggcgtgc ggcggatcga tcgcgaaggt ccatcggttc ggaacgctca tggcccaagt
      961 ggaagcgcct ggacatgagc ccccacgttc tatagacgca gtgtggcggc cgcggcgcct
     1021 accaactagt gggtagtcaa agcgccggcg agtcgggatg ctgcctgctc accccaggcg
     1081 ggcggccgcg tctactgatt gaaagccgcc gggaataccg agcggccggg atagccagag
     1141 cagcgctgcc acgcatctat caaattggaa acagaaaatc cggagcccgc aaaaacgagc
     1201 tccgcaaagc tgaaaacagg gaggcattat gagtaatgtt tggacgcggt ccagaagagc
     1261 gttcgcattg ctgatcacca gtgcagcgct gggggcgctg gttgttgccg gagcggtggt
     1321 ccattcatcg acaggagcaa acccaccggc ttcgtttcaa tttgcggacc cccacgaggg
     1381 cccgagccgg aatagctttg cgccggtggt gaaaaagacg ctgccggccg tggtgaacat
     1441 ctccacttcc aagatcgtga agacgccaac ggggttctcg ggcgagatgc cgatggaccc
     1501 ttttttccga agatttttcg gcgatgaatt cggcaattcc ttcaatgcgc cccgcgagcg
     1561 gcgcgagcgc agcctgggat cgggcgtcat cgtaagccgc gagggttata tcctcaccaa
     1621 taatcacgtc atcgacggcg ctaccgacat tcgtgtcacg ctgtccgata agcgggaact
     1681 gaaggctcgc ctgatcggcg ccgacgcgaa gacggacatc gccgttctga aagtcgatgc
     1741 cgccaatttg ccattcatgg tgcttggcga ttcatccaag gctcaggtcg gagatttcgt
     1801 cctggctatc ggcaacccgt tcgggctggg cggcacggtg actatgggga tcatcagcgc
     1861 cacgggccgc ggcaatctgg gcattgagga ctacgaagat ttcatccaga ccgacgctcc
     1921 catcaatccc ggaaactcgg gcggagctct ggtcaatgac cgcggggagc ttatcggcat
     1981 caataccgcc atcctttcgc gatccggagg caaccagggc gttggcttcg cggttccgat
     2041 caatctggca cggacagtaa cggatcagat tcttaagacg gggcatgtga cccgggggta
     2101 tctgggtatt atgttgcagc cggtgactcc ggccattgcc aaagcttttg ggctgaaaga
     2161 ggcgcaggga gccctggtcg ccgatgtgga gcccaatagc cccgcgcaga ggagcggcgt
     2221 tgaaaagggc gatatcattc tggccgtcaa cggcaaggct gtaaccgacg tgaatcagct
     2281 tcgcttgagc atctcgatga tggcgccagg atcggctgtg gacctcaaag tgtttcggaa
     2341 tggcgctgac cgggagatac gggcggagct gggcgagatg ccgtccaagc aggaacgcgc
     2401 gtcgctcgag aacaacggca gcggcggagc tttggacgga gtggatgtgc aggatttgac
     2461 gccggagact gcgggcgctc tccatctccc gcctcagacc aggggtgtgg ttgtgacacg
     2521 aatcgaccca gccagcgagg cggcgggatc aggcctccag gaaggggatg ttattcaaga
     2581 ggtcaatcat cagccagtgc gaaatagcac cgactttaac cgcgcgctgc ggcagtcggc
     2641 gcaggcgggg aaaccctccc tcttgttggt agaccggcag ggaacaactt tgtttattgc
     2701 ggtctgagca ggttgcagcc tcctgccttc ggcgccccag gccgagtcct gttgtatttt
     2761 ggcaggctgg ctgctaatag ggcggactgg tgtctgcgtg ggccggacgg atacagacct
     2821 tacgaggatg catgaagcct atgtatccgc ccgccgccga tatgctgaag agcttgcgaa
     2881 gaccattact ttgctcaggc tgggcgaatt cgcggacgcc ttcgtaggcc agcgagcgag
     2941 ggagatggaa gccttcgagc gcttccggat tgcccgccag gtctatctgc attacatcca
     3001 ggacaaggag cgcgaagccg cgtaggctgc ggcgctaagg tgagcaattt ctccatggcg
     3061 gaactgctcc tgcccaggat ttgtgtccag gcccccgctt ccggcaagcg caagcgtatt
     3121 cctgcattcc gaatggaccg cgtaaatttt tgccgtcggt cctaagacca ggtgatatga
     3181 ttgtgccaat cactcaggca catctatgaa tccatcggat agcctccctg cggaactcat
     3241 tcagaaagtt ttggcgcaag cgcctcctgg atcgtggatc atcgcgaggc gatcggatgg
     3301 aaagattctc ggaatcggca aaaccggcag ccaggaagcg ctacagaggg cgcaagccga
     3361 tcacccggac ctccgcttgg atgaggtggt ggtgctttgg aaatcggccc cgggcaaaga
     3421 gcccgaaacg ccgccacagt ccagatccaa agcaggttaa accaggacgc cggcccatgg
     3481 ttctggatag gcatgggttg attcggatgt ggcgccgcag gagctgcaca ggcgatacgg
     3541 acagcgcgag ccagggcgaa tgccaagcga agagcgaaat ggtacgtgga atcgcagcgg
     3601 ggcagaacgt ctccgcccca aggacggcgt tccgctgccg aaacgcgagt ttgtggaacg
     3661 catgctgctt gcgtgagagt ctggccggcc cgagggcggt cgagcaggat taccggccgt
     3721 atgtaggtga actgcggcaa ggacggctgg agccggcgga tggggcggac cgtggaggcc
     3781 gctgaaaacg aaccagccgg gaaaggccaa gcgcacacca cagctcgtcg tcaggggcgc
     3841 cgcgcatgcc gccagatctc gaagccatag tcaacggcat gcatccgccg gggagcgatg
     3901 cgccggatgg cgtctgggag aggaattccg actggctaag ctagtctcaa ttgcgggacg
     3961 aaatagcgtt tggaaatgtg acacggccgc cggcgatcgg caaaatcccc ctgaacgcaa
     4021 acaacgagta ctccgcaggg attggcgatc tctcaatcta cctgcccata agtagcgagc
     4081 gcggccagta tgaaattcgc ttgggaagcc ggcgagccgg tttttggaac gcaagggact
     4141 gcgtccatcg agaacgggac gactgtcgtg agagcgaaac tggacctcca tcgtctcgca
     4201 cctggccaat atttcttcgg catccggcag ccgccgtggg agtggacgta cgtgccaatc
     4261 gttatccgct gatcgataac acaaacctgc gtaacatgca ctctatcttc atccgcgctc
     4321 gggaagcgca acgacgttca cacagcgcag caagaaagtt tggcgacatc cttttcgaaa
     4381 gtgagagcgg ccagcttgat gccctccacc atagtcagat aaggatgaaa ggtgtgcgta
     4441 atatcttcga acgtgagccc gaatcgaatg gccagtgtgg cctcctgaat aacctcgcct
     4501 gcgtctgcgg cgagcgcatg agcgccgagc aggcggccgg tcgcctgttc ggcgaccagc
     4561 ttgatcagcc cgcgcgtgtc tcgggacgcc tgcgcgcgcg gcacatcctt caggctcaac
     4621 accgcagtct tgatcttcag gccttgcgcc cgggcttgac tctccgtaag cccgacgctc
     4681 gccatttgtg gatcggtgaa ggtcaccgaa ggcaaggcgg agagatcata caagcgcccg
     4741 gcgccggtta aggcgttctc ggcggccaat ccgcccgcgt aagctgcgac atgcacatac
     4801 atcggatcgc cgatgcaatc gcctgcggcg tagatgtccg gattggttgt ttgcagatat
     4861 tcattgacca ggatttcccc acggccgccg gtttggaccc cggcggtctc caggccgaac
     4921 ccctctgtgt ttggccgccg gcccgttgcc acaagtagct gcttggccgt acacacctcc
     4981 ggccgcccgt ccagcctcgc gtggacttca tactgatcgc gcttgcggtc aatccgctta
     5041 atcaccgcat tcacacacgc gcgaatcttt tccccctcca ggtactgcgt caacgccgtc
     5101 ccgatttcgg gctccgaggc cgccgccaga tggggttgcg cttcgaaaac catgacacgc
     5161 acgccaaagc ggccgaacaa ctgggccagc tcaagaccaa cggaccccgc cccaatcaca
     5221 atcatcgatt ccggcaactc cggcaggcta agcgcagccg tgctatctaa ataaggaatc
     5281 tcagcaagcc agggtattgg cggcgcccag ggatgcgagc cgctggcgat cagaattttg
     5341 cccggcgtgt accgatgccc gtttacctcc agactccgcc cgcccgtgaa acgagcctcg
     5401 cctttgagga tcgtgatatt ggcatattgt tcggccacgt gcacatactt gtcccgccgc
     5461 aacatggcca cgagttcgtc tttttgctcg acgacccgcc gccagtccga aggcggagga
     5521 caagccgtta gaccttgaaa attcggatac gctgagtgat agcacagctc ggctgcttta
     5581 atgagcgtct tcgaaggcac acaacccacg ttgacgcagg ttcccccaat cgttcctttt
     5641 tcgataatcg ccacgcgggc gccgagttcc gctgctttga tcgctgcggc gaaagccgct
     5701 gagccgccgc caatggtcat ccaatcaaat gactcgccgg aggaaggaac gcggcgcccg
     5761 ttcccgaccg gccgcttttc cgttgccacg gcgtggtagc ccgccttttc caccgcggcc
     5821 tctaagctct tactgtctac ttcctgaccg gccaccacag cggcctgccc ggatttccag
     5881 gagccgacgc gaacgtcggt gactcccggc acgctccgca acgcttccgt cacatgccgc
     5941 gcgcagttct cgcacgtcat accttcgatc cggagtgtaa actgcgcctg gttcgccatg
     6001 agatgccctc ctaggcgata ttgatcaggg cttgcacgcc ggagcgcact gctggcgcat
     6061 aagcacgttg agaacgctgg cgaggatcag tcccgcgatc ccaagatacg ctagcgccgc
     6121 gctgtggcgc ataagagtga agagatacgc aatcacgccg cttcccagcg gcaatgacca
     6181 gagcccaaag ccgccggtga actcgaaagc caaacagcag ccctatgaac gcaaccgcca
     6241 gaaatgcaat catcagcgga cgcaggacag cgtcgttgat gagaaacccc agtcccagcg
     6301 cggccagaat cgacacaatg gcgggcagtc ccatgcagca cgccgcggcg acaaaggacc
     6361 cagcaatgcc aaccttatcc aaatggtccc ttaaagacac ggctctctcc ttcctaggtg
     6421 caaggcggcg ccgactgttc cttagagcct gccttgacat acttgcgcca aatccattcc
     6481 gggcaataag cgagaagggt tccgacaatg gcggcggcaa aaatccgccg ccatgagagc
     6541 gccagtactt ccaatccccg cagaaggctc cctgttgcga gcagaaggta cagcggaccg
     6601 gtaacgtaac agtgcaggcg accgcagcga gcggcgttca ctacacaggc tgcgcctgct
     6661 acggcaaagg cggaagcccc atagccaggc gcgtcccgcg ggccagcagc accctgctac
     6721 aaacgcaagc cagggaatgc cccacagcat ccaagcgacg gtggatccca ctaggtcgac
     6781 agacctgaaa ctctgagcca atc
//
LOCUS       DASREK010000310        23112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2129616, whole genome shotgun sequence.
ACCESSION   DASREK010000310 DASREK010000000
VERSION     DASREK010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 23112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23112
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129616"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /gene="bamA"
                     /locus_tag="VFA54_16690"
     CDS             complement(<1..1779)
                     /gene="bamA"
                     /locus_tag="VFA54_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HZS52508.1"
                     /translation="MQLQAAAGAIRSRIVSAAIVVLLFSLSKTWGQAPSPGNPKPPAS
                     QQQPQQKPPSPFEEVPEAKPEAPKPPAQPPSQQKSPFEAVPEEKKEAPPPAAPSAPGA
                     PAAPQGPARQDIIERIEFRGNRRIPRDTLMARLYSKQGDVYDPEQLRRDFMLLWNTGY
                     FDDVRLEVEPGTLGQIVRFVVVERRMIRSIKYEGNKSVTVSEILDRFKERKVGLAVET
                     RYDPTKIARAANVLKEFLGERGRQYATVTPEIHQIPPSSIEVVFNVVEGPKVKVGRID
                     IVGNTVMSDRQAVRAMKNLRPIGIPHSIILENLFAKTFDQSKLEEDKDRLRDAYQQRG
                     YFRATVGEQTLTMRNVGGSGLRLPVFYPNKPGKRADLRIPVEEGKQYHVGRITFSDVK
                     LFRTPEAVLRPYFQMQEGAIFDVSKLRKGMENLKKVYGEFGYIDFVSEPNFEFREDEK
                     PPKIDLNLTVDEGKQFFIRRINFSGNTTTRDKVIRRELLLDEGDMFNTRLWDLSILRL
                     NQLGYFEPLKAEEAAQIDRDTRNGLVDLTLKVKERGKNSVGLTGGVSGFAGSFIGFNY
                     ETNNFLGLGETLTLQTQLGTIQRSALF"
     gene            complement(1959..2660)
                     /locus_tag="VFA54_16695"
     CDS             complement(1959..2660)
                     /locus_tag="VFA54_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52509.1"
                     /translation="MRVAMAAWSWPRTRLPIGFVLIPFFCLSPGPAPAAETKAPGGWF
                     SAQSMDVEISARLIIDKQAVIDAVGSDLKRQYSLVELKVTPKGNYPVTLSRDDFLLRS
                     ERDNQHATADSPDLIAGPAVLVLTDTGATRPGYASPGMPTGIPGLSGRKRKKNRDKTP
                     EQGAVSGEDAQSEQSEKTSASLIAALQAKELPLGETRKTVSGYLYFPVDPQQKAKNFY
                     LSYKGPNGVCEIRFK"
     gene            2732..3115
                     /locus_tag="VFA54_16700"
     CDS             2732..3115
                     /locus_tag="VFA54_16700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZS52510.1"
                     /translation="MVKYTNFHHVSFASKNLAASRAFFGEVLGLEEINRPAFTFPGAW
                     YALGDRQLHIIENQEAGGTTPARQSRSDHVALEVPDIAAVKNALESHGIGFEEGTNQQ
                     LGMAQLFCRDPDGHVIEFIQFLHKG"
     gene            3127..3744
                     /locus_tag="VFA54_16705"
     CDS             3127..3744
                     /locus_tag="VFA54_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HZS52511.1"
                     /translation="MRNLLILVLGCAWAAFGAAAAYGQAAEASLSMGAGILKNNDLGS
                     LGVTVNGVEQRLSVGNGFRLSARLSLNTWEHFGHEFGYGYNHTSLQVGTQGSVGMGVH
                     QGFYDFMVYATPTDSKVRPFVCGGGGFASFFPPGSSAFSGNGITKFSYNYGGGVKAKI
                     TSIYGVRFDIRDYVTGKPFGSSSGVTDVHGLLHNVEVSAGFEILF"
     gene            3863..3938
                     /locus_tag="VFA54_16710"
     tRNA            3863..3938
                     /locus_tag="VFA54_16710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3896..3898,aa:Ala,seq:cgc)
     gene            4093..4920
                     /locus_tag="VFA54_16715"
     CDS             4093..4920
                     /locus_tag="VFA54_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZS52512.1"
                     /translation="MVKTEQEHRQDIIEVGRLVYQKEWIASNDGNISIRLDSGRILAT
                     PTGVCKGMMRPEDLIILDYEGNKIEGRLKETSEILMHLTIYKMRPDVGAVVHAHPPVA
                     TGFATSGRALDQALLPEVIIGLGCVPLAAYGLPGTPALTEPMLPFIPKYDAILMANHG
                     VVSYGATVWNAFFNMETVEHCARINLVAELLGGATVLPRREVEKLFASRPRYGVKTQT
                     QPEPSLPVVAEDLESAKNAGGARRSEGATASADGEERYCVTRGELLALVNEALRTKR"
     gene            complement(5086..5628)
                     /locus_tag="VFA54_16720"
     CDS             complement(5086..5628)
                     /locus_tag="VFA54_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52513.1"
                     /translation="MSKRIGTALALGAWLAATLFMWLVATQNFHLVDRLLAAPSPDFA
                     RRIAGLASGEARLLLRYQASEVNRWFFDRWGWTQLGLGVILSILVSRAAFPRKIRISI
                     FLMLLIAAVLQIYVVPEIVRLGRLLDFAVRTPPPPQEVFFWRLHAAYTALDMLKFLLG
                     VYVAAGLLRDKSWADAAQRS"
     gene            5741..6166
                     /locus_tag="VFA54_16725"
     CDS             5741..6166
                     /locus_tag="VFA54_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52514.1"
                     /translation="MLRLLLALLRLSCPASSQSRSWSGTWTATGAGRVFSGTWTASAG
                     ESRDASSGTWIVLDSSGKPVAAGAWSARKKKKGWEGVWQARAGNGAPFAGTWSARSPL
                     EASAALTDLWELALSNVITGAWQAGRKSGGWSIRAFRSR"
     gene            complement(6194..6928)
                     /locus_tag="VFA54_16730"
     CDS             complement(6194..6928)
                     /locus_tag="VFA54_16730"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HZS52515.1"
                     /translation="MPDAILKVFRGDAQGGEMVEYRVPLSPGMVVLDAMHYIQAHLAP
                     DFAVRWNCKAAKCGSCSAEVNGRPRLMCKTRMDSLPLDQPVTVLPMKSFPVIKDLVTD
                     VSWNFKVNKKIPPFTPAKNVEWKMFQPDVDRVQEFRKCIECFLCQNVCHVLRNHDKTE
                     QFGGPRFFVRVAGLEMHPLDSLSRTRLLKEDLGIGYCNITKCCTEVCPEDIHITDNAI
                     IPLKERVVDDFYDPLRMLVRKIKGAK"
     gene            complement(6957..8672)
                     /locus_tag="VFA54_16735"
     CDS             complement(6957..8672)
                     /locus_tag="VFA54_16735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZS52516.1"
                     /translation="MDKYETHEHDVLIIGAGGAGLRAAIEALAQGARVGVVCKSLLGK
                     AHTVMAEGGIAAAMAHVDPADGWKTHFKDTMRGGKFLNNWRMAQIHAQEAPDRVRELE
                     QWGALFDRTDKGEILQRAFGGHTYKRLCHVGDRTGLEMIRTLQDRGVSLGVDVYMECT
                     VSRLLTDGGRIAGAFAYWREQGRFVVFKAKAVVIATGGIGKAYHVTSNSWEYSGDGMA
                     MAYQAGAELMDLEFVQFHPTGMVWPPGVRGILVTEAVRGEGGILRNKNGERFMERYDP
                     KRMELSTRDVVARAIYTEVREGRGTEHGGAYLDISHKPAEYVKRKLPSMYHQFLELAD
                     VDITKQAMEVGPTCHYVMGGIGVDAETAQSTLPGLFAAGEAAAGLHGANRLGGNSLSD
                     LLVFGRRAGASAARYACSSPPAVVDSGQIEEAARELLEPFERNGGENPYDIQRDLQDV
                     MQNRVGIFRNEEDLKLALDELEKLKARLAKVSVQGSRLYNPGWHLAQELRSLLTVSEA
                     VARCALERKESRGAHSRIDFPNYDERWAKLNNVISRDGDQMKLTQRPTPEMPRELQEL
                     LAEDQ"
     gene            complement(8672..9430)
                     /locus_tag="VFA54_16740"
     CDS             complement(8672..9430)
                     /locus_tag="VFA54_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52517.1"
                     /translation="MAESRVTLEGKGFGATLRRDAWWTELLPVIILLGLFGIYATVRA
                     FEGNYYEWGPYLSPFYSPLIDAQHQWWPLSPAILILPFPLLFRVTCYYYRKAYYRAFF
                     LDPPACAVGEPGEHRYQGETSFPFILQNLHRYFFLIALVILAILWYDAIVAFDFDGHF
                     GIGLGSLIMLVNVLLLTGYTLGCHSLRHLIGGKLDCFSCAVAGGPRHNAWKWVTRLNE
                     HHMFWAWTSLISVGSTDAYIRMLAAHAITDLRLL"
     gene            complement(9577..10353)
                     /locus_tag="VFA54_16745"
     CDS             complement(9577..10353)
                     /locus_tag="VFA54_16745"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HZS52518.1"
                     /translation="MEFKNLLLAATPPVATITLHRPSRRNALSVEMMQELIACLDEVS
                     AQKDVRVVILAAEGPAFCSGHDLSEMTGQPIHRYRYIFDICTELMTRIRSIPQPVIAQ
                     VQGIATAAGCQLVAACDLAVASETASFATPGVKIGLFCSTPMVEVSRAVGRKRALEML
                     LTGKTIDARTACDWGLVNCVAPEADLAQAAWRLACEIASASPLTVGLGKQAFYTQIDL
                     DQPRAYAYAKEVMSMNATAADAQEGIQAFLEKRRPCWVGK"
     gene            10570..11778
                     /locus_tag="VFA54_16750"
     CDS             10570..11778
                     /locus_tag="VFA54_16750"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS52519.1"
                     /translation="MKLANTERSLLPGRLLAALSLGAIVWAQSAPSQAGSGQTSSPLV
                     TDQAARRLKAVQAKSGEVMSIEDYQPRSTLVVPQHPVPRAKYPFIDIHSHHRTPADQA
                     YLRKLLSEMDTINLRILVNLSGSTGDKLKKGIEAFREASPDRLKMFANLDFSAIDEPD
                     YGRRAAAQLEEDVKNGAVGLKIFKNLGMDLHYKDGRRVPVDDPMLDPVWEACGRLKIP
                     VLIHTAEPWSFFQPIDKYNERWLELKTHPGRARPPDKYPSWETLMAEQEHIIAKHPHT
                     IFISAHLAWRGNNLAALGSLLDRYPNMYTECGAVLYDLGRQPLTAHDFLVKYQDRVLF
                     GKDTYAVNEYPYYFRVLETRDEYFDYYRKYHAFWQMYGLGLPDEVLKKLYYKNALRIV
                     PGLNPTGFPE"
     gene            11870..14887
                     /locus_tag="VFA54_16755"
     CDS             11870..14887
                     /locus_tag="VFA54_16755"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HZS52520.1"
                     /translation="MSPKSERLDFAAHVREAMRFSSAYRLKGELVERALARGGRAGLL
                     ESYFGAEDYQELRELAYRAAERSVRGGPRVLILPGILGSTLGRRRPILDDVIWINPIE
                     IARGRLAALALRTSPGCGSYEALGAVLFAYLKLKLRLRIAGFDADFYPYDWRQNIDVL
                     GNRLCRRLESEKASAIQIVAHSMGGLVCRAAIQSGAKKIGRLILLGTPNFGSFASVQA
                     LRGTYPVVQRVALLDLKHSASDLAANVFNTFPALYAMLPWPEKLQGFDLFNASAWPRG
                     GPRPAQKMLDEAKQIQQQLAPADERFFLIAGVNQETITGLRCDGEEFIYETSRAGDGT
                     VPLDLAELPGAKTSYVEESHGSLPNNPVVARAAADLLGAGRTDALPASWQPSSSGLIR
                     RISESDLRASHDPGRAGSLGPAEIRRLCKEWISPDAARLSSESRPAARVSSSTFEPIV
                     IGRRRQRRLELRLAYGSIADVSARGYVLGVYRDVAPSGAARELDARLEGTITEFARRR
                     MFAANAGEVFLLPAARHAIRGDWILLAGLGPFDRFSGDVQQLVGEQVVRTLVRTNVEE
                     FATVLPGAGSGWSVAAGLENLLTGFIRGLRNADRDRRISGLTVCEIDRVRYEEIKREL
                     CRLSMTSLFGDFEISLDEVRLPAAISVTGHSGRTRDASTRLEPVYLLVRQEQPGRFRS
                     SLLGAGEKATVVTAVKEAPGARVDQLLSQIQNLRCAALADFGDQLAGLTLPGEIASLL
                     QEFRNHHVVVVHDAASSRLPWEAIRIAGRFPAAEAGLSRKYIAENLSVAKWLEQRQLD
                     SVLNLLLVVNPTADLDGADREGRRMLRLFDAHAAIRTEQLRGAAATKTAVLDAFRSGR
                     YDVIHYAGHAFFDPATPAHSGIVCHGGEVLSGADLAGIGNLPSLVFFNACEAGRIRRR
                     GGAAYRAIEQNTGFAEAFLRGGVANYVGTYWPVGDRAAEAFAAAFYGGLAGGQPIGEA
                     LAAARRVVKDEIQSPDWAGYMHYGAHDFVLKQR"
     gene            complement(14923..15624)
                     /locus_tag="VFA54_16760"
     CDS             complement(14923..15624)
                     /locus_tag="VFA54_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HZS52521.1"
                     /translation="MKYKRHESPEARFEPFEKNFSDGVSVHGFLHRPPERCIGGLVLT
                     HGAGSNCRSPLLLAMAGAFAGAGFLVLRCDLPFRQARAKGPPLRSSDQDREGLRRAAL
                     AMRDLLAEPAAISGGGRAQRVFLGGHSYGGRQASMAAAANPELVDGLLLLSYPLHPPR
                     KPNQLRTAHFSRIQGPALFVHGSRDPFGTLEEMRGALALIPAKTRLLPMEGAGHELSA
                     DASFPTLAAFQELIG"
     gene            15688..17400
                     /locus_tag="VFA54_16765"
     CDS             15688..17400
                     /locus_tag="VFA54_16765"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZS52522.1"
                     /translation="MTGSPLPPLCTRLLAVILTLISSLWADGVIIHSVDVRGTRRPLV
                     LETQAGEALDTARVSRDVHRLWATGWFSDVRVESTETPAGPDIVFHLTERPRFYLRRV
                     EFEPKKEKQALGLEPGSPIDDFRAQKIANSIKTALVEDGYANAKVNAEVAPVGPDQAD
                     LRLRIEEGSQCRIREARFTGNTALPETDLRRALRATRVRRILFWKTHPPLTSSAVDAD
                     VARLQSFYLSRGYFDARVQQGPVQKEGELARVTYSIDSGPQYKTPEGVNFSAGALCRC
                     LFQQRRESEKEGKLDFAARIEAEEIRDERLVHLTTSIDAGPAYTVGRIDFRGNHKFRD
                     GTLRRAMLLDEGALFNLDLLRRSVVRLNRMNIFERIDENSVLIARQPSAGRANIVISV
                     KEKRPGVWSISGPVGPPSIAGPLQATVASRLPAWGRGPFETSTYFATLSLVGFYRPLV
                     KFLPYTLPKPFFPVLGIQRPFLPGQQWQSGFFLSPQLAPPAMLASYGIAQFRGRVTLG
                     SSSTPPPVVAQVERTIRARQDAAPEPAEPRPLICEMPRSRWAPLRSAANFLLNLLPAY
                     LVGL"
     gene            complement(17382..17768)
                     /locus_tag="VFA54_16770"
     CDS             complement(17382..17768)
                     /locus_tag="VFA54_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HZS52523.1"
                     /translation="MLQRIIAPGAASPRGPYSHAVRAGDFIYVSGQGPLDPATNQFKL
                     GDVGSETRQTLENIRRILQACGADLQDVVKCSVFLTDVKDFAAMNQVYSEFFGDIRPA
                     RTTVQATLPGLGTKVEIDCIAYKPTR"
     gene            complement(17856..18950)
                     /locus_tag="VFA54_16775"
     CDS             complement(17856..18950)
                     /locus_tag="VFA54_16775"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZS52524.1"
                     /translation="MHISELETPALLIDLDRMEANLCRVAQYAKQHSLRLRPHTKTHK
                     TPAIGRKQIELGAAGLTVAKVGEAEVMLSSGTPDLLVAYPVIGESKLRRLMQVARQTK
                     LTVALDSVEAARELSDAAKSAGVNVAVLVEADVGMHRVGVAVGEDLVALAREVTRLSN
                     LTLEGVACYPGHIRSAKEENKPLLERLARDIERIVQDFRRAGLPLKIVSAGSTPLVFQ
                     SHQFPGINEIRPGTYVFNDINCVRSGMATLEDCAVTILTTVVSNARPGYVIVDGGSKT
                     FSSDHLAGSNEASFGYVIEAPQALFYNMNEEHGFLDVRECGRPFRIGERLRVIPNHVC
                     VAMNLHEQVYGIRGDQVEQVFRVEGRGKLQ"
     gene            19189..20277
                     /locus_tag="VFA54_16780"
     CDS             19189..20277
                     /locus_tag="VFA54_16780"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZS52525.1"
                     /translation="MSRTILVAGEDLGVCSHLKRLLTARNYEVVTAGGGLAALAEFHR
                     SRPDLLLLETAVRGRDGFDVCRQLKNNPQTRLTPVILIGGAPATKARLQGIDAGADGV
                     LTKPLNIQELLAWVRSLLRLKAYTDELDSAEAILFALARTIEGRDPRLEGHCERLAEY
                     SSRLGKILGLAPEQITALRRAGIVHDIGKVAVPDSILLKPARLTAEEWKIMREHPLVG
                     EKICAPLNSFRLVLPIIRHHHEKMDGSGYPDGLRGNEIPITARTLQLVDLYDGLTTQR
                     PYRRALRHSEALARIEREVEEGWWDARIFCKMKEMFHNGNGRARRRSGAPAEQKSRVF
                     ALSPARATAYDPLLEIEAGLRHRAAGVR"
     gene            20382..21077
                     /locus_tag="VFA54_16785"
     CDS             20382..21077
                     /locus_tag="VFA54_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS52526.1"
                     /translation="MQALPTTEKAPARSTIRIAIADPHTLFRAGLRKLLAAEEDFDVV
                     AEAGEGSEILPLLETYHPDVLLLDLYLGGVAGLDVLRHLQAAGTIGRHSGTRILVLTA
                     SEDEEALAQTMKLGASGVVLKTTATEMLMQAIRKVSSGEMWLDARVTARVIKNLAEPR
                     AAGRGYEDATGLTPREREIAALVGQGYRYREIAEMLSISEQTVKNHLRNMFHKLGVSD
                     RLQLALYAIHHNL"
     gene            21121..21570
                     /locus_tag="VFA54_16790"
     CDS             21121..21570
                     /locus_tag="VFA54_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS52527.1"
                     /translation="MKKQTPTVLVVDDDPADQFLVQEAMKIVNLNYNLRLVSDGDEAL
                     DYLFGRGRYGDRFRAPRPDLILLDLNMPRFNGRLVARAIKSNPYLKKIPVVVLSTSSQ
                     EEDVEELYSIGVNSYLQKPANFDEFTAAMRDLSNYWLERASLPPQWI"
     gene            complement(21673..22221)
                     /locus_tag="VFA54_16795"
     CDS             complement(21673..22221)
                     /locus_tag="VFA54_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HZS52528.1"
                     /translation="MDLINSYAIVAYVAGPVARFAERLRNELSPGCPHRAHITVLAPR
                     PLDSPLTEAIEFARQLIAQFEPFDVRLNGIRNFRETQVIYLALAEGVSEFRAMHDVLN
                     SGVLHQPDAYNYTPHITLGQQLPEGTFEHSMRLSSQRWRQFGPPPVLHIDTLTLVQQQ
                     ADGSWKDLEEFTLGHVAAVGKT"
     gene            complement(22503..>23112)
                     /locus_tag="VFA54_16800"
     CDS             complement(22503..>23112)
                     /locus_tag="VFA54_16800"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HZS52529.1"
                     /translation="YLLPKVFAAFKKKHPEVKITIYRNFTTKIVQKLQEDSLDLGFVS
                     LPVDVKEIATIPLFQEWVDVAMPPDHPLASQRSVTVAEIARYPWIFPRASRTREMLDE
                     MFRAAGTPVHVTMELSGVETIKRFVSIGLGISLLSRSYASAEVKAGLLTLVPLAEPHL
                     KRELGLAYRTDRYLSRSTQAFIEMARKHFGQAGKGEGKPAVT"
BASE COUNT         4586 a         7073 c         6931 g         4522 t
ORIGIN      
        1 aaacagagcc gagcgctgga tcgtgccgag ctgggtttgc agcgtcagcg tctcgccgag
       61 tccgaggaaa ttgttcgtct cgtaattgaa cccgatgaag ctgccggcaa acccggaaac
      121 gccccccgtc agcccaaccg agttcttccc gcgctccttg actttgagcg tgagatccac
      181 caggccattg cgcgtgtcgc ggtcgatttg cgcggcttct tctgctttga gaggctcgaa
      241 atacccgagc tggttgaggc gcaaaatgct caagtcccaa agccgggtgt tgaacatatc
      301 gccctcgtcg agcagcagct cgcgccggat caccttgtcg cgcgtggtcg tattgcccga
      361 aaaattgatg cggcggatga agaactgctt gccctcgtcc acggtcaggt ttaggtcgat
      421 tttcggcggc ttctcgtcct cccggaactc gaaattgggc tcggacacaa aatcgatgta
      481 accgaattcg ccgtagacct tcttgaggtt ctccatgcct ttgcggagct tggaaacgtc
      541 gaagatcgcg ccctcctgca tctggaaata ggggcgcagc acggcctcgg gagtgcggaa
      601 cagctttacg tcgctgaacg tgattctgcc cacatggtac tgcttgccct cttccaccgg
      661 aattcgcaga tcggcgcgtt tgcccggctt gttcgggtag aagacgggaa ggcgcaagcc
      721 gctgcctccg acattgcgca tggtgagcgt ctgctcgccc acggtggcgc ggaagtagcc
      781 gcgctgctgg taagcgtcgc gcaggcggtc cttatcctct tcgagcttgc tttgatcgaa
      841 cgtcttcgcg aaaagatttt ccagaatgat cgagtggggg atgccgatcg gccgcaggtt
      901 cttcatggcc cgcaccgcct gccggtctga catgacggta ttgccgacga tgtcaatccg
      961 cccgaccttg actttggggc cctccaccac attgaacacc acctcaatcg acgacggcgg
     1021 aatttggtgg atttcgggag tgaccgtcgc gtactggcgg ccgcgctcgc ccaggaattc
     1081 cttcaacacg ttagcggccc gcgcgatctt cgtcgggtcg tatcgcgttt ccacggcaag
     1141 tcccaccttg cgttccttga agcgatccag aatctcggaa acggtcaccg atttattgcc
     1201 ctcgtatttg atcgagcgga tcatccgccg ctcaacgaca acgaagcgca caatctgacc
     1261 gagcgtgccg ggctcgacct cgaggcgcac gtcgtcgaag tagcccgtat tccacaagag
     1321 catgaaatcc cgccgcagct gctcggggtc gtatacgtcg ccttgcttgg aataaaggcg
     1381 ggccatcaag gtatcgcgcg gaatacgccg gttgccccga aactcaatgc gttcgatgat
     1441 gtcctgccgc gccggccctt gcggggctgc cggagcgccg ggagcagagg gcgccgccgg
     1501 cggcggagcc tccttctttt cctcgggcac agcctcgaat ggagatttct gctgagacgg
     1561 cggctgcgct ggcggctttg gggcctccgg cttggcttcg ggcacttctt cgaacggaga
     1621 cggaggtttt tgctgcggct gctgctgaga agcgggcggt ttcggattgc cgggagacgg
     1681 cgcctgaccc caggttttgc ttaatgaaaa cagcaaaaca acaatggccg cggacacaat
     1741 ccggcttcga attgctcctg cggccgcttg caactgcata ttaggcccag tcctttcgcg
     1801 ctatgtatct ttctcttaaa cgcacgctca ttttctatgg ttacacactc gcgcggccat
     1861 ccggtaattt tacacagcct gccgcgcgct tcacacgtcc cacccatata cccggcgagg
     1921 acgtccggcc ccgcagagcc tctcgcggta agcctggatt acttaaagcg gatttcacag
     1981 actccgttgg gacctttgta gctcaggtaa aagttcttgg ccttctgctg aggatcaacc
     2041 ggaaagtaaa gatagccgct caccgtcttg cgtgtctcac cgagaggcag ttccttcgcc
     2101 tgcaatgccg cgatcaaaga cgcgctggtt ttttccgatt gctcggactg cgcatcttct
     2161 ccggaaaccg ctccttgttc cggcgtcttg tcgcggttct ttttccgctt tcgtccgctc
     2221 agccccggta tgcccgtggg catgcccgga cttgcgtaac cgggccgtgt cgcgccggta
     2281 tcggtaagca cgagcacggc gggaccggcg atgaggtccg gggaatccgc cgtggcgtgt
     2341 tggttatccc gctcggaacg gagcaggaaa tcatcgcgcg acagcgttac cgggtagttt
     2401 cctttcggag tcaccttcag ttccaccagc gaatattgcc tcttcaaatc ggacccaacc
     2461 gcgtcgatca ccgcctgctt gtcgatgata aggcgggccg aaatctcgac atccatgctc
     2521 tgggcggaga accacccgcc gggcgcttta gtttctgcgg ccggagcggg gcccggagac
     2581 aggcagaaaa aggggatgag aacaaaaccg atcggcagcc gcgtgcgcgg ccagctccag
     2641 gcagccatag cgacccgcat cgcccattat cacacaaaga gggcccgact ccctgtgtta
     2701 agatggtcgc gaagcacacc ttccgttcgg tatggtcaag tacacgaatt ttcatcacgt
     2761 gtcgttcgct tcgaaaaatt tggccgccag tcgcgcgttt ttcggggagg tactggggct
     2821 ggaagaaatt aacaggcccg ccttcacgtt tccaggcgcc tggtatgcgt tgggcgaccg
     2881 ccagctacac atcatcgaga accaggaggc gggcgggacc acgccagcgc ggcagagccg
     2941 ctccgatcac gtcgcgctgg aagttccgga catcgccgcc gtgaaaaacg ctctcgaatc
     3001 gcacggcatc ggcttcgagg agggaaccaa tcagcagctt ggcatggcgc agctattttg
     3061 ccgcgaccct gatggacacg tcatcgagtt catccaattt ctacacaagg gctgacaaca
     3121 atccatatgc ggaatctgtt gattttagta ctaggatgcg catgggccgc tttcggcgca
     3181 gccgctgctt atggccaagc ggcggaggcc tctctttcga tgggcgccgg cattctgaag
     3241 aacaatgatt tgggcagctt gggcgtgacg gtaaacggcg tagagcaaag gctgagcgtc
     3301 ggcaatgggt tccgcctttc ggctcgcctc agcctcaata cctgggagca cttcggccac
     3361 gaattcggct acggctataa ccatacgagc cttcaagtgg gcacgcaggg cagcgtgggg
     3421 atgggagtcc accaagggtt ttacgatttc atggtttacg cgacgccgac cgacagcaaa
     3481 gtgcggccct tcgtatgcgg ggggggcggt tttgcgagtt tcttcccacc gggcagctcc
     3541 gcgttttcgg gcaacggcat cacaaagttc agttacaact acggcggagg cgtcaaagcc
     3601 aagatcactt ccatctacgg cgtccgcttt gacatccgcg attatgtaac gggcaagccc
     3661 ttcggcagct cgagcggggt gaccgacgtg cacggactgc tgcacaacgt ggaggtctcg
     3721 gccggcttcg aaattttgtt ttgaacgttg gccgcgcaga gcttgcctgc tcgggaagga
     3781 tcgccgcttt ggcgcagcct tccgaagagc gccggaacgc gccgccacga cgtgctatag
     3841 tgagtacgaa taaaccccgg gtgggcggct agctcagttg ggagagcacg gcgttcgcaa
     3901 cgccggggtc gagggttcga atcccttgcc gtccaccaaa tatcttctcc tgcggacgct
     3961 ccttttttcc aaaccgccgc cgcgcgcaac gacccttgca ggtatttgca atgcccagca
     4021 ccgccgcggc ctgactcacc ttcgagagcc tttccaaccg ccgcggggcc acgctacaat
     4081 acaaaatctc tgatggtaaa gaccgagcaa gagcaccgcc aggacattat cgaggtcgga
     4141 cgcctcgtct atcaaaagga gtggatcgca tccaacgacg gcaatatttc catccggctc
     4201 gattccgggc gcatcctggc tacgccgacc ggcgtatgca aagggatgat gcggcccgaa
     4261 gacctgatca tcctcgatta tgaaggaaac aaaatcgagg gccgtctcaa agaaacctcc
     4321 gaaatcctca tgcacctgac gatttacaag atgcggccgg atgtgggagc cgtggtgcat
     4381 gcgcatccgc cggtggccac cggcttcgca accagcggaa gggctctcga ccaggctctg
     4441 ctgcccgaag tgatcatcgg attaggctgc gtgccgctcg ccgcttacgg cttgcccgga
     4501 acgcccgctt tgaccgagcc catgctgccg ttcattccca agtacgatgc cattctgatg
     4561 gccaaccacg gcgtggtctc ctacggcgcg actgtatgga acgctttctt caatatggag
     4621 accgtcgaac attgcgcgcg gataaatctg gtagccgaac tgctgggcgg cgcgaccgtg
     4681 ctgccacgcc gggaggtaga aaaactgttc gcctcccgtc ctcgctatgg cgtcaagacg
     4741 cagactcagc ccgaacccag cttgcctgtg gtggcggagg atctcgaaag cgccaagaac
     4801 gcaggcggcg cacggcgctc cgaaggcgct accgcctcgg ccgacgggga agaacgctat
     4861 tgcgtcaccc gcggcgagct gctggctctg gtgaacgagg cgctccgcac gaagcgctaa
     4921 aagcacctgg cccgctcaag ccccgcgacg ggccccaaag aaacgccgct ggcaagggcc
     4981 agcgtgcttt cgggatatcg ctcgacccct ggtgacgatc ccccaaagtt cgctcccggc
     5041 gaatcccagc ctccgcggtg aggctcgagc cgcacgcttc ccggctcagg acctctgggc
     5101 ggcgtccgcc caggatttat ctcgcaacaa gccggccgct acgtaaacgc ccagtaaaaa
     5161 cttgagcatg tcgagagcag tgtaggcggc gtgcaggcgc cagaagaaga cctcttgggg
     5221 cggtggaggc gtgcggacgg caaaatccag cagccggccc agccggacaa tttcgggaac
     5281 cacataaatc tggagtacgg cggcgatcag cagcatcaag aagatggaaa tacgaatttt
     5341 ccgggggaaa gccgcccgcg agacgagaat cgaaagaatg acgccgaggc ccagttgggt
     5401 ccacccccag cggtcaaaga accagcggtt tacttccgag gcttgatagc gcaggagaag
     5461 ccgggcttcg cccgaggcca gcccagcgat gcgccgggcg aaatccggcg agggcgccgc
     5521 gagcaaccgg tcgaccaaat gaaaattttg agtggccacc aaccacatga agagcgtcgc
     5581 agccagccag gccccgagcg ccagcgccgt tccaattcgt ttgctcatgg cggcatgatc
     5641 gcgatcttgc cggctgcgcg tcaaggcgcg attttcgaat caacctcgct gaaaactgcc
     5701 cacgttgcct acaacacctc ccgagtctac tctattagtc atgttgcgcc tgcttttagc
     5761 gctgctccgc ctgagctgcc ccgcctcttc tcaatcgcgt tcctggagcg ggacctggac
     5821 ggcgactggg gccgggagag tcttcagcgg gacgtggacg gccagcgcgg gcgaaagccg
     5881 cgatgccagc tccggcacat ggattgtttt ggatagctcg ggcaagccag tggctgccgg
     5941 cgcctggtcg gcgcgcaaaa agaaaaaggg ctgggaaggc gtttggcaag cccgggctgg
     6001 caacggcgcg ccctttgcgg ggacttggag cgcccgttca cccctcgaag cgtcggccgc
     6061 gcttacggat ctttgggaac tggcgctgag caacgtgatc acaggcgcct ggcaagcggg
     6121 caggaagtcg ggcggctggt cgatccgggc tttccggagt cgataggccg cagcgcaagg
     6181 cgagcttcct ctttcatttg gctcccttta tttttctaac gagcattcgg agcgggtcgt
     6241 agaaatcgtc caccacgcgt tctttgagcg ggattatggc gttatcagtg atgtggatgt
     6301 cttccgggca aacctccgta cagcacttgg tgatgttgca atagccgatg ccgagatcct
     6361 ctttcagcag ccgagtacgg gaaaggctgt ccagcggatg catctcgagt cccgcgacac
     6421 gcacgaaaaa gcgcgggccg ccgaactgct ccgtcttgtc atgattgcgc aacacatggc
     6481 agacgttctg gcacaagaaa cactcaatgc acttgcgaaa ctcctgcacg cgatcgacgt
     6541 caggctggaa catcttccat tcgacgttct tggccggcgt aaagggcggg atttttttgt
     6601 tgaccttgaa attccaggag acgtctgtaa ccagatcctt gatcaccgga aaggatttca
     6661 ttggaagaac ggtgaccggc tgatcgagag ggagagaatc catgcgcgtc ttgcacatca
     6721 gccggggcct gccgttcacc tcggcggagc atgatccgca cttggcggct ttgcagttcc
     6781 agcgaacggc gaaatcggga gcaagatgcg cctggatgta gtgcatggcg tccagcacga
     6841 ccatgccggg ggaaaggggg acgcgatatt cgaccatttc gcccccttgg gcgtcgccgc
     6901 gaaaaacttt cagaattgcg tcaggcatcg aaaatctccg ccaatttttt atcccattac
     6961 tggtcttccg ccaacagttc ttgcagctcc cgcggcattt cgggcgtagg acgctgcgtg
     7021 agtttcatct gatcgccgtc gcgcgagatc acgttgttaa gcttggccca tcgctcatcg
     7081 tagttcggga aatcaatgcg gctgtgggcc ccgcggcttt cctttcgttc cagagcgcag
     7141 cgggcgacgg cttccgagac cgtcagcagc gaacgcagtt cctgagcgag atgccagccg
     7201 gggttgtaaa gccgcgagcc ctgcacgctg accttcgcca gccgggcttt cagcttttcc
     7261 aattcatcga gcgccagctt gaggtcttct tcattgcgga aaatgccgac ccggttctgc
     7321 atcacgtcct gcaaatcgcg ctggatgtca tagggatttt cgcctccgtt acgctcgaac
     7381 ggctcgagca gttcccgcgc agcctcttcg atctgaccgc tgtccaccac ggcgggcggg
     7441 gaactgcacg cgtaccttgc agccgaggct ccggcgcgcc gtccgaaaac cagcaaatcg
     7501 gagagggaat tgcctccgag ccggttagcc ccgtggaggc ccgccgccgc ctcgccggcg
     7561 gcgaacaggc cgggcagagt ggattgcgcc gtctccgcgt ctacgccgat gccgcccatc
     7621 acgtaatggc aggtcggccc cacctccatc gcctgcttgg tgatatccac gtcggccagc
     7681 tcaaggaatt gatgatacat actgggcagc ttccgcttca cgtactccgc gggcttatgc
     7741 gagatgtcga ggtacgcccc gccgtgttcg gtgccgcgcc cctcgcggac ctcggtgtaa
     7801 atcgcgcggg cgactacgtc gcgggtggaa agctccatgc gcttgggatc gtatcgctcc
     7861 atgaagcgct cgccgttctt attgcgcagg atgccgccct ccccgcgcac agcttcggtc
     7921 accaggatgc cgcgcacacc cgggggccag accatgcccg tgggatgaaa ctgaacgaat
     7981 tccagatcca tcagctcggc gcccgcctga taggccatgg ccattccgtc gccggagtat
     8041 tcccaggagt tggacgtgac gtgataggct ttcccgatgc ctccggtggc gatgacgacg
     8101 gctttcgcct tgaacaccac gaagcgccct tgctcgcgcc agtaagcgaa agcgccggct
     8161 atccggccgc cgtcggtgag cagccgcgag acggtgcact ccatatagac atccacgccc
     8221 aggcttacgc cccggtcttg caaggtgcgg atcatttcca gcccggtcct gtctcccacg
     8281 tggcacagcc gcttgtaggt gtgcccgcca aaggcgcgct gcaagatctc gcctttatcc
     8341 gtccggtcga acagggcgcc ccactgttcc agttcccgca cgcggtcggg cgcttcctgc
     8401 gcgtggatct gggccatgcg ccagttattg aggaatttcc cgccgcgcat agtgtccttg
     8461 aagtgcgttt tccagccgtc ggccggatcc acatgagcca tggcggcggc gatgccgccc
     8521 tcggccatga ccgtatgcgc tttccccagc aaagacttgc aaaccactcc cacgcgcgcc
     8581 ccttgcgcca aagcttcaat cgccgcccgc aagccggccc cgccggcccc gataatcaac
     8641 acatcgtgtt cgtgagtttc gtatttgtcc atcacagcag cctcaaatca gtgatggcgt
     8701 gagctgccag catcctgatg tacgcgtccg tactgcccac ggaaatcaag ctcgtccagg
     8761 cccaaaacat atggtgctcg ttcagccggg tgacccattt ccacgcgtta tggcgcgggc
     8821 cgcccgccac cgcgcaggag aagcaatcca gcttgccgcc aatcaaatgg cgcagcgagt
     8881 ggcagccgag ggtgtagccg gtgagcagca agacattcac cagcatgatc agcgagccca
     8941 gcccgatccc gaaatgaccg tcaaaatcaa aggccacgat cgcgtcatac cagagtatgg
     9001 ccaggatcac cagagcgatc aggaaaaaat accggtggag gttttgcaag atgaagggaa
     9061 acgacgtctc cccctggtag cgatgctctc ccggctctcc gaccgcacag gctggcggat
     9121 cgaggaaaaa cgcacgataa tacgccttgc ggtagtaata acacgttacg cggaagagaa
     9181 gcgggaaggg caaaatcaag atcgccggcg agagcggcca ccattgatgc tgcgcgtcaa
     9241 tgaggggaga atagaagggc gagagatacg gcccccattc ataataattt ccctcaaacg
     9301 cccggacggt ggcgtagatg ccgaacagcc cgagcaaaat gatcaccggc aacaactcag
     9361 tccaccaggc gtcccgccgc aatgtggcgc cgaacccttt accctccagc gtgactcgag
     9421 attccgccat tagcccccct aaacttctat cctaatgtat tcgggaaaca actcggtgcg
     9481 aagtaagccg ctagtagacc acgctgcgcg ccgcagccgc aagggctctc gggaaattag
     9541 ccgccgtatt tttcccgaac ctgcccgctt cccattttat ttccccaccc agcatggccg
     9601 ccgtttttcc agaaatgctt ggatgccttc ctgcgcgtct gccgctgtcg cattcatact
     9661 cattacttcc ttggcgtacg cataggccct cggctggtcg aggtcgatct gcgtataaaa
     9721 agcctgcttg cccaagccaa ccgtgagcgg gctcgccgac gcgatttcac aagcaagacg
     9781 ccaggccgct tgcgccaggt ccgcctcggg cgccacgcag ttcacgagtc cccaatcgca
     9841 ggccgtccgt gcatcgatcg tcttgcccgt cagaagcatt tcgagcgccc gcttgcgccc
     9901 cacggcgcgg ctcacttcga ccatcggcgt gctgcaaaaa agtccgattt tcacccccgg
     9961 cgtggcgaac gaggcagtct ctgacgctac cgccagatcg caagccgcta ccaactggca
    10021 gccggccgct gtggcgatgc cctgcacctg ggcaatgacg ggctgcggga tggatctgat
    10081 gcgcgtcatc agttccgtgc aaatatcgaa gatgtagcga taccggtgaa tcggctggcc
    10141 ggtcatttca ctaagatcgt ggccggagca aaacgccggg ccctcggcgg ccaggatcac
    10201 gacgcggacg tctttctgcg cgctgacctc atccaggcag gcgattaatt cctgcatcat
    10261 ctcgacggac agggcattgc gccgcgacgg acgatgcaac gtgatcgttg ctacgggcgg
    10321 agtcgcagcg agcagaagat ttttgaactc catacctcca gcctagcgcc tggcggccgc
    10381 ctccgcaacg ggccggcggg cggttcctcg agcatcccag gttgaacgcg ctcggagaat
    10441 taccgagcca atgcgcctgc gttacgagtt cgggctacga ggctcgcttg tgcgggtcat
    10501 ctacgcagaa cggccatgca gcgcgctggc ccggttgcaa ccgaaatttc ttgtgttacg
    10561 cttacggcta tgaaattggc gaataccgag cgttctctgc tgccggggcg cctgcttgcc
    10621 gcgctcagct tgggcgcgat tgtttgggct caaagcgcgc caagccaagc cggctccggg
    10681 cagacttcct ctcccctggt cactgatcaa gccgcccggc gactcaaggc cgttcaggcc
    10741 aagtccggcg aagttatgtc aatagaggat taccagccgc gctcgacgtt ggtcgttccg
    10801 cagcaccctg tgcctcgcgc caagtatccc tttattgaca tccacagcca ccaccgcacg
    10861 cccgccgacc aggcgtattt gcgcaaattg ctttcggaaa tggacacgat caacttgcgg
    10921 atcctcgtca acttgagcgg cagcacgggc gataagctga aaaagggaat cgaagctttc
    10981 cgcgaggcga gcccggaccg cctgaagatg ttcgccaatc tcgatttcag cgccattgat
    11041 gagccggatt acggccgccg cgccgccgcg caactcgagg aagacgtgaa aaacggcgcg
    11101 gtgggcctga agatttttaa aaacctcggc atggaccttc attacaagga cggccgccgt
    11161 gtgccagtgg atgatcccat gctggatccg gtatgggagg cgtgcgggag attgaaaatt
    11221 ccggttctga tccacacggc tgagccgtgg tcatttttcc agccgatcga taaatacaac
    11281 gagcggtggc tggagctcaa aacgcatccc ggccgcgccc ggccgcccga caagtatccg
    11341 tcctgggaaa ctctgatggc cgagcaggaa catatcatcg ccaagcatcc ccacaccatc
    11401 ttcatttccg cgcatctggc gtggcgaggg aacaatctcg ccgccttggg atctctgctg
    11461 gaccggtatc ccaatatgta cacagaatgc ggcgccgtac tgtacgacct cggccgccag
    11521 ccgctcaccg ctcacgactt cttagtgaaa taccaggacc gcgtcctgtt cggcaaagac
    11581 acgtacgcgg tcaacgagta tccttactac tttcgcgtat tagagacgcg cgacgaatat
    11641 ttcgattact accgtaaata tcacgcgttc tggcaaatgt acggcttggg tctgcccgac
    11701 gaggtattga agaagctgta ttacaagaac gctctgcgga tcgtgccagg actcaatccc
    11761 acaggttttc ccgagtaatc gccggcattt agctccggct cgtttggctg cgccccgtac
    11821 aatgtaagta gtcggggtga aaatgcaacc aaaatcgagg cggggatcag tgtcccctaa
    11881 aagcgaacgg ctggacttcg cggcgcacgt gcgggaagcg atgcgtttca gctccgcgta
    11941 ccggctgaaa ggcgaactgg tggagcgggc gctggcgcgc ggcggccgcg ccggccttct
    12001 ggaaagctat ttcggcgcgg aggactatca agagctgaga gaactggcgt accgcgccgc
    12061 cgaacgcagc gttcgcggcg ggccgcgggt gcttatattg cccggcatcc tgggctcgac
    12121 actcggccgg cgccggccga ttctcgacga cgtcatctgg ataaatccga ttgaaatcgc
    12181 tcgaggacgg cttgcggccc tggcgcttcg caccagcccc ggttgcggct cctacgaagc
    12241 cctcggagcc gttttattcg cttaccttaa gctcaagctc aggctgcgaa tcgcgggttt
    12301 cgacgccgat ttttatccgt acgactggcg gcagaatatc gacgttttgg gcaaccggct
    12361 atgccggcgg ctggaatccg agaaggcgtc ggcaattcaa atcgtcgctc atagcatggg
    12421 cggccttgtg tgccgcgccg cgattcaatc cggggcgaag aaaatcggcc ggctcattct
    12481 gctgggaacc cctaatttcg gatcttttgc ttcggtacag gcgcttcgcg ggacctaccc
    12541 tgtggtccaa agagttgctc tgctggatct caagcacagt gcgagcgacc ttgctgccaa
    12601 cgtgttcaac acctttcctg ccttgtatgc gatgctgcca tggccggaaa aactccaagg
    12661 tttcgacctt ttcaatgcgt cggcatggcc caggggcggg ccgcggccgg cgcagaaaat
    12721 gctggacgag gcaaagcaaa tccaacagca gctcgctccg gccgatgaac gatttttctt
    12781 gatcgccgga gtcaaccagg aaacaattac cggcttgcgc tgcgatgggg aggaattcat
    12841 ctatgagaca tcccgcgccg gcgacggcac cgtgccgcta gatctggccg agctgcccgg
    12901 cgcgaagacc agctacgtgg aagaatcgca cggcagcctg ccaaacaatc ccgttgtagc
    12961 ccgggccgcc gccgacttgc tgggagcggg gagaacggat gctcttcccg catcgtggca
    13021 gccgtcgagt tccggcttga ttcgcagaat ttccgagagc gatctccgcg catcgcatga
    13081 cccaggccgc gcgggcagtt tgggacccgc ggaaatcaga cggctttgca aggagtggat
    13141 ttcccccgac gctgccaggc tctcctcaga atcgagaccg gccgctcgag tgtcttcctc
    13201 taccttcgag ccgatcgtca tcggccggcg ccggcagcgg cgtctggaac tgagattggc
    13261 ctacgggagc atagcggatg tcagcgcgag gggatatgtg ctcggggttt accgtgatgt
    13321 cgcccccagc ggagcggcgc gggaactgga cgccaggctc gaggggacca tcaccgaatt
    13381 cgcccgccgg cggatgttcg ccgctaacgc aggcgaggtg ttcctattgc cggctgcgag
    13441 gcatgcaatt cgtggcgatt ggattttact ggccggtctc ggcccgttcg accgcttcag
    13501 cggcgacgtg cagcagctcg ttggcgagca ggtggttcga actctggtcc ggaccaatgt
    13561 cgaagagttt gccacggtct tgcctggagc gggatccggc tggagcgttg ccgcaggttt
    13621 ggaaaatctg ctgactgggt ttatccgagg cttgcgaaac gccgatcgcg acaggcgcat
    13681 cagcggcctt acggtttgtg aaatcgatcg ggtccggtac gaagaaatca agcgcgaact
    13741 atgccggctt tcgatgacct ctttgttcgg cgattttgaa atcagcttgg acgaggttcg
    13801 gctgcccgcg gccatttctg tgaccgggca ttccggacgc acccgagatg catccacgag
    13861 acttgagccg gtttatttgc tggtacggca ggagcagccg gggcggtttc ggtcctcgct
    13921 gctcggcgcc ggcgaaaaag cgaccgtggt caccgccgtg aaagaagcgc cgggagcgcg
    13981 cgtcgatcaa ttgctctcgc aaattcaaaa tctccgatgc gcggcgctgg cggatttcgg
    14041 cgaccagctc gcgggcctga ccttgcccgg cgagatcgcc tctctgctcc aggaattccg
    14101 gaatcatcac gtcgttgtgg tgcacgatgc ggcgtcgtcc cgccttcctt gggaagccat
    14161 acgcatcgcg ggccgcttcc ccgcggccga agccggcttg agccggaagt atatcgccga
    14221 gaacttgtcg gtcgccaagt ggctcgaaca acggcaactc gattcggttc tgaatctctt
    14281 gctagtggtg aatccaacag cggatttgga tggcgcggac cgcgaaggcc ggcgcatgct
    14341 caggctcttt gatgcgcacg cagcgatcag gacggagcaa ctgcgcggcg ccgcagcgac
    14401 caaaaccgcc gtgctggatg cctttcgctc gggccgctac gacgtaatcc actatgcggg
    14461 gcacgcgttt tttgatccgg ccactcccgc gcacagcggc attgtctgcc acggcgggga
    14521 ggtgttgagc ggagcggatc tcgccgggat tggcaatctt ccgagtctcg tattcttcaa
    14581 tgcctgcgag gcaggccgga tacgccgaag aggcggcgct gcgtaccgcg caatcgagca
    14641 gaacacgggc ttcgccgaag cctttctgcg cgggggcgtc gcgaattacg taggaactta
    14701 ctggccggtc ggcgaccgcg ccgcggaagc gttcgccgct gcattttacg gcggcctagc
    14761 cggcgggcaa ccaatcggcg aggcgcttgc ggccgcgcgc cgggttgtca aagacgaaat
    14821 acagtccccc gactgggccg gctacatgca ctacggcgct cacgattttg tgctgaaaca
    14881 gcggtgagga tcgccggacg tttcatcgaa cgaatgctgt tttcaaccga tcaattcctg
    14941 aaaggcggcc agggttggga aagacgcgtc cgctgaaagt tcatgtcccg cgccttccat
    15001 gggaagcagt ctggttttcg cggggataag agcgagggcg cctcgcatct cctccagcgt
    15061 tccgaagggg tcccgcgaac catgtacgaa gagcgcgggt ccttgaatgc gggagaaatg
    15121 ggccgtgcga agttgatttg gttttcgcgg cgggtgcagg ggataggaaa gcagcagcag
    15181 gccgtccacg agctccggat tggcggcggc agccatgctg gcctggcggc cgccgtagga
    15241 atgacctccg agaaacaccc tctgggctct cccgccgccg gagattgcgg cgggttcggc
    15301 aagaaggtcc cgcatcgcca atgcggcgcg ccgcaaaccc tcgcgatcct gatccgaaga
    15361 gcgcagcggc ggacccttgg cccgagcctg gcggaaaggc agatcacacc gcagcacgag
    15421 aaatccggct ccggcgaaag cgcccgccat tgccagcaac aggggagagc ggcagttcga
    15481 tcccgcgccg tgtgtcagca ccaggcctcc gatacatcgt tctggggggc ggtgcagaaa
    15541 tccgtgcaca gagacgccat cgctgaagtt tttttcaaac ggctcaaagc gagcttcggg
    15601 cgattcatga cgtttatatt tcacgggcgt taaaaaaacc ggcgaagacg aaacggaaaa
    15661 cgctaagtcc gcgtcccaga ttgttcaatg acaggctccc cgctgccgcc gctctgcacg
    15721 cgtctgcttg ccgtcattct cacgttgatt tcgtccctct gggccgatgg ggtgattata
    15781 cattcggtgg atgtgcgagg gacccgccgg cccctggtgc tggaaacgca ggctggagaa
    15841 gcgctggaca ctgcccgggt cagccgcgac gtgcaccgtc tctgggccac gggctggttc
    15901 agcgatgttc gtgtagagag caccgaaacg ccggctgggc ccgacatcgt tttccatctt
    15961 accgaaagac ctcgttttta cctgcggcgt gtcgagttcg agccgaaaaa agaaaagcag
    16021 gcactcgggc tggagccggg gtcgccgatc gacgatttcc gtgcccagaa gatcgctaac
    16081 tcgatcaaaa ccgccctggt tgaggacggc tacgccaatg ccaaagtaaa cgcggaagtg
    16141 gcgcccgttg gacctgacca ggcggacttg cgcctgcgaa ttgaagaagg ctctcaatgc
    16201 cggatccgcg aggcgcgctt taccgggaac acggctttgc cggagaccga cttgcggcgc
    16261 gccctgcgcg cgacccgcgt gcggcgcata ttgttctgga aaacccaccc gcccttgacg
    16321 tccagcgccg tggatgccga cgtggcgcga ttgcagtcgt tctacctttc ccgcggctat
    16381 ttcgacgccc gcgtacagca gggtccggtt cagaaagagg gcgagctggc acgcgtaacc
    16441 tattccatcg attccggccc gcaatacaag acgccggagg gggtcaattt ctcggccggc
    16501 gcgctatgcc gctgtctgtt ccaacagcga cgggaatcgg agaaggaagg caagctggat
    16561 tttgcagcgc gcatcgaagc cgaagagatc cgggacgagc gactggtcca tttaacaacg
    16621 agcatcgacg ccggcccggc gtacacggtt ggccgtatcg attttcgggg aaatcacaag
    16681 tttcgagacg gcactctgcg gcgggcgatg ctgctcgacg aaggcgcttt attcaacctg
    16741 gatttactgc ggcgcagcgt agtccgtctg aaccggatga acatttttga gcgcattgac
    16801 gagaacagcg tgctgatcgc gcggcagccc tcggctggcc gggcgaatat cgtgatttcc
    16861 gtgaaggaga agcgcccggg cgtctggtcg atttccggtc ccgttggtcc tccttcgatt
    16921 gccggtccct tgcaggctac ggtcgcctcg cggctgccgg cctggggccg gggaccgttt
    16981 gagacttcca cttacttcgc gaccctgagc ctggtgggct tttatcgtcc tctcgtgaag
    17041 tttttgccgt acacgctccc caagccgttt ttcccggtgc taggcatcca gcgccctttt
    17101 ttgcccggac agcaatggca atcgggcttc ttcttgtccc cgcaactggc tccgccggca
    17161 atgctggcga gttacggaat cgcgcagttt cgcggccgtg tcacgctggg aagcagcagc
    17221 acgccgccgc ctgtcgtcgc gcaagtggaa cgcacaatcc gcgcgcggca ggatgcggcg
    17281 ccggagccgg cggaaccgcg cccgctcatc tgcgagatgc cccgatcgcg ctgggctccg
    17341 ctgcgatcgg cggcaaattt tttgctgaat ctgctgcccg cctatctcgt gggcttgtaa
    17401 gcaatgcagt caatctcgac tttggtgccg agccctggca gcgtcgcttg cacggtggtc
    17461 cgggcaggac ggatgtcgcc gaaaaattcc gaatagacct gattcatcgc ggcgaagtcc
    17521 ttgacgtcgg tgagaaacac cgaacacttg accacatcct gaagatccgc cccacaggcc
    17581 tgtaaaatcc ttcgaatgtt ttcgagggtc tggcgtgtct cactgccaac gtcgccgagc
    17641 ttgaactgat tggtggctgg atcgagcggt ccctgccccg agacatagat gaagtctcca
    17701 gcccgaaccg cgtgcgaata gggtccccga gggctggccg ccccgggggc gataatcctc
    17761 tgtaacatcc gttctctcct gatgttttgg acttgaactg cggagttttc gcgggatctc
    17821 gtcctggacg cccgtgcagg caacgcagcg aaatttcact ggagtttgcc ccggccttcc
    17881 acccggaaca cctgctctac ctggtcgccg cgaatgccgt agacctgctc atgcagattc
    17941 atcgccacgc acacatgatt ggggatgacg cggaggcgct ccccgattcg gaaagggcgg
    18001 ccgcattccc gcacgtccag aaagccgtgc tcttcgttca tgttatagaa cagggcctgc
    18061 ggcgcctcga taacgtagcc gaaactggcc tcgttggagc ctgccaggtg atcggaggaa
    18121 aacgtcttgg aaccgccgtc cacgatcacg tatccggggc gggcgttcga gactaccgtc
    18181 gtcaggatgg tgacggcgca atcctcgagc gtggccatgc cgctccgcac gcaattgata
    18241 tcgttgaaca cgtaggtgcc gggccggatc tcattaattc cgggaaactg gtgggattga
    18301 aacacgagag gcgtagaccc cgcgcttact atcttcaagg gcaggccagc tctgcggaaa
    18361 tcctggacga tgcgctctat atctcgggcc aggcgctcca gcagcggctt gttttcctct
    18421 ttggctgacc ggatgtgacc ggggtaacac gccacgccct ccagggtgag attggacagg
    18481 cgcgtgactt cccgggccag agctaccaaa tcttcaccca ccgccacgcc gacgcggtgc
    18541 ataccgacgt cagcttccac cagcacagca acgtttacgc cggcggattt ggcggcatcc
    18601 gacaattccc gagcggcctc gacactgtcg agcgccaccg tcaacttggt ttgacgcgcg
    18661 acctgcatga gccgccgcag cttgctctcg ccgatgaccg gataggccac cagcagatca
    18721 ggggtaccgc tggatagcat gacttcggct tcgcccactt tggctaccgt caagccggcg
    18781 gctcccaatt caatctgctt gcggccgatg gccggcgtct tgtgggtctt ggtgtgcggg
    18841 cgcaggcgca gactgtgctg tttcgcgtac tgcgccacgc ggcagagatt cgcttccatg
    18901 cggtcgagat ctataagaag cgccggcgtt tccagttccg agatatgcat aatccttgaa
    18961 aattgccagc ttatcacagg caaagacttc ctccatccgt gtctcgggcg gcgccgcggc
    19021 gcagcttcct gggccggcgg atcggtctcc cggcggcgag cttttgtctg gcgcccgatc
    19081 ccggaggtcc ttccggcacg gccgatgtaa gggatggaac gccgaagaat aggagtatcg
    19141 ctctatgttt ccagttcttc cgatgtggga aagtaagaaa ttaggcccat gtcgagaacg
    19201 attctggtcg ccggcgagga cctgggggtg tgctctcacc tcaagcgcct gctcaccgcc
    19261 cggaattacg aagtggtaac cgcaggcggg ggcctggcag ccctggcgga attccaccgc
    19321 tcccgcccag acttgcttct gctggaaacg gcggtgcgtg gacgcgacgg cttcgacgtg
    19381 tgccggcaac tgaaaaacaa tccgcagacg cggctcaccc cagttatcct gatcggcgga
    19441 gcccccgcca cgaaagcccg cttgcaagga atcgacgctg gagcggacgg cgtcctcacc
    19501 aagcctctga atattcagga actgctcgcc tgggtgcgtt ccctgctgcg cctgaaggca
    19561 tacaccgacg agttggacag cgcggaagcc atactatttg ctctggcacg aactatcgaa
    19621 ggcagagatc cccgcctgga gggccactgc gagcgcctag ccgaatactc ctcccgcctg
    19681 ggcaaaattc tgggcctcgc tccggaacaa atcaccgccc tgcgccgcgc cggcatcgtg
    19741 catgacatcg ggaaggtagc tgtgcctgac tcgattctcc tgaagccggc gcgcctgacg
    19801 gcggaagaat ggaagatcat gcgggagcac cccctcgtcg gcgagaaaat ctgcgctccg
    19861 ctgaattcat ttcgccttgt actgccgatc atccgtcacc accacgaaaa aatggatggg
    19921 tccggttatc cggacggcct gcggggaaac gaaataccga tcacggcccg cacgttgcaa
    19981 ctggtggacc tgtatgacgg cctgacgaca caacgcccct accgccgcgc tttgcgccat
    20041 tccgaagcct tggcccgcat cgagcgcgag gtcgaagagg gctggtggga cgcccgcatt
    20101 ttctgcaaaa tgaaggaaat gtttcacaac ggcaatggcc gagcgcgccg ccgcagcgga
    20161 gcccctgcgg agcaaaaaag ccgcgtattc gcgctgtctc ctgcccgagc cactgcctat
    20221 gatccgctgt tggaaatcga agccggattg agacatcggg ccgcgggcgt ccggtaacga
    20281 attcgccccc gccgcactac tgttccccga caagcaagcc gatttgagca actgaggcgc
    20341 cgcaatggca cgcaggggcc ctcaaggaga aaagccgccg cttgcaagcg ctccctacga
    20401 ctgaaaaagc gccggcccgc agcaccatcc gcatcgctat cgccgacccg catacgctat
    20461 tccgggccgg cctccggaag ctgctggcgg cagaggaaga tttcgacgtg gtcgctgaag
    20521 ccggggaagg atccgaaatt ctgcctcttc tcgaaaccta tcatccggac gtcttgctgc
    20581 tcgatttata tctgggcggg gtcgccggct tggacgtgct gcgccatttg caagccgcgg
    20641 gcaccattgg ccggcacagc ggaacccgca tccttgtgtt gacggcctct gaagacgagg
    20701 aagccctcgc ccagaccatg aagctgggcg cttccggagt cgtcctgaag accacagcga
    20761 cagagatgct gatgcaggcc attcgcaagg tctcgagcgg agagatgtgg ctcgacgccc
    20821 gagtcaccgc ccgtgtgatc aaaaatctgg ccgagccccg cgcggcaggc cggggatacg
    20881 aagatgcaac cgggcttacg ccgcgagagc gggaaatcgc agccctggtg ggccagggtt
    20941 accggtatcg ggagattgcg gagatgctct ccatcagcga acaaaccgta aaaaatcatt
    21001 tgcgcaatat gttccataaa ctcggggttt ccgaccggct ccaactggcg ctctacgcca
    21061 tccatcacaa cctctaattt ttcatcaagc cgccggattt ttgaccgata agaaattgct
    21121 atgaagaaac agacgccaac cgttctggta gttgatgacg acccggccga ccagttcctg
    21181 gtccaggagg ccatgaagat cgtcaatctg aactacaacc tgcgcctcgt ttcggacggc
    21241 gacgaagcgc tcgattactt gttcgggcga ggccgctacg gagaccgctt ccgcgcaccg
    21301 cgtcccgacc tcattctgct ggacttgaac atgccccgtt ttaacggccg gctggtggcc
    21361 cgcgcgatca aaagcaatcc ctacttgaaa aaaattcccg ttgtcgtgct cagcaccagc
    21421 tcgcaagaag aagacgtgga ggagctctac tcgatcggcg tgaactcata cctgcaaaag
    21481 ccggcgaact ttgacgaatt taccgccgcc atgcgggatt tgagcaatta ctggctcgaa
    21541 cgagcgtcgc tgccgccgca gtggatctaa acaaaaaggg atcggaatac aaaacgctcg
    21601 aaattgccgc gccgcactgc cgtagccagg gcggcgggcg gctgattcgc ctcttccgag
    21661 aaccggttgc aatcaggttt tccccacagc ggccacatgc ccgagggtga actcctcaag
    21721 atccttccag cttccatcgg cctgttgctg tacgagcgtc aaagtgtcga tgtgcaaaac
    21781 gggcggcggg ccgaactggc gccagcgctg gctcgacagc cgcatactgt gctcaaacgt
    21841 accctcaggg agctgctggc cgagcgtgat atggggcgtg tagttgtagg catccggctg
    21901 gtggaggact ccggagttca gcacatcgtg catcgccctg aattccgaga cgccttccgc
    21961 caaggccagg tagatcacct gtgtctcgcg gaagtttcgg atgccgttca gccgcacatc
    22021 gaaaggctca aattgggcga ttagctggcg ggcaaactcg atggcctcgg tgagagggct
    22081 gtccagggga cgaggagcta gaaccgtgat gtgagcgcgg tggggacagc ccggtgataa
    22141 ttcgtttcgt agcctctcag cgaatcgcgc gaccgggccg gctacgtaag ccacgatcgc
    22201 gtaggaattt ataagatcca ttgcgaaggc tcccctttta tcccccctgt tcttttgttg
    22261 taccttgaaa cgcttcccga tacaacccgg taccgggccg ggaggcatgg ttttcactca
    22321 agccggttgt ccgcgtgatt ggccctttcg gaggcggcgc cctcgagcgg gcataggccg
    22381 ggacaaatgg tcatatatcc attcaccggc gggatgtccc agcagtacga aaaaagttag
    22441 aaagagcaaa agcggcaaac accatttaac ggctgtctga tccggcgggg cgcttggagc
    22501 attcaggtca cggccggctt tccttctccc ttgcccgcct ggccgaaatg ctttcgcgcc
    22561 atttcaatga aggcttgcgt ggatcgcgac aaatagcggt ccgtgcgata cgccagaccc
    22621 agctcgcgct tgagatgggg ttcggccagc ggcaccagcg tcagcaggcc ggccttgact
    22681 tcggcgctgg cgtaactgcg gctcaacaac gaaatgccca gcccgatgct gacaaatctc
    22741 ttgattgttt ccactcccga cagctccatc gtgacatgca cgggagtgcc cgccgcgcga
    22801 aacatttcat ccagcatctc gcgagtacgg ctggcgcgag gaaaaatcca cggataacgc
    22861 gcgatttccg ccacagtgac gctgcgctgg gaggccaacg ggtgatccgg gggcatggcc
    22921 acatcgaccc actcctgaaa caggggaatg gtcgcgatct cttttacatc gactggaagg
    22981 ctcacaaacc caagatcgag cgagtcctcc tggagcttct gaacgatctt ggtcgtgaag
    23041 ttgcgataga tggtaatttt cacttccggg tgcttcttct tgaaagcggc gaaaactttc
    23101 ggcaataggt ag
//
LOCUS       DASREK010000311         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_974701, whole genome shotgun sequence.
ACCESSION   DASREK010000311 DASREK010000000
VERSION     DASREK010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974701"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFA54_16805"
     CDS             complement(<1..361)
                     /locus_tag="VFA54_16805"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS52530.1"
                     /translation="MRGKVVLLEFFQTTCPHCQHTAGIIEPIYKEWRSRGLEILAVAI
                     NPGAIHLIDDFRRRFAATYPIGVGDPTMVRTFADISAVQSFFVPYVFMIDRKGVIRAE
                     HGGMDQAFWSHQEANLRD"
     gene            complement(612..1877)
                     /locus_tag="VFA54_16810"
     CDS             complement(612..1877)
                     /locus_tag="VFA54_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52531.1"
                     /translation="MKKRLALPWLLLAVLLAALIHRPAASAPQAAADTQITAGQEAQL
                     FSRIDEIFNETSQIMGLKIRRKVPRAVISREKIREYIEQRMSETLPPEDLRVEEIVLK
                     KFGFIPQDYDLKAQTVDLLTEQAAAFYDFKEKKLYLASWTPSGMQDVALVHELAHALA
                     DQHFNLEKFIEKSGDDDDASTARGAVVEGQASWVMTEYMLRQMGKSLRDAPQLAASAL
                     EATAEGAKEYPVFGKSPLYLQETLMFPYTQGMLFQQAVIQKLGREAFTEVFKHAPVST
                     QQVLHPERYFARSMPSKPALPRAELPPGYKKIAEGTMGELDFQILLRQFLGEEEARDL
                     APKWRGSRYVLWEDKKGHRDVLGYAVEWSSDADASRYFQDYRKVCGLKWKHMTVSGSE
                     GHQATGAGDDGRFAWSQHGAVFTSVEGMP"
     gene            complement(1874..3229)
                     /locus_tag="VFA54_16815"
     CDS             complement(1874..3229)
                     /locus_tag="VFA54_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS52532.1"
                     /translation="MGESTGEQELSAALGGGAELASGPAVPSGVAEGVSGEAAVLPQK
                     GVRQVFRALRHRNFRLFCFGQIISLCGTWMQNVAQTWLIYRLTRSEFLVGLTYFCLQI
                     PVFALAPLGGIVSDRRSRHRIVIATQTLSMVQAFLLGALTVSGRVQVWQVLALAVALG
                     CINAFDMPARQAFIVEMTSKEDLLNAISLNSSMFNAARVVGPGIAGVLVGWLGEGVCF
                     LLNGASFIAVIISLLLMRLQPFERSVADSPWDHLIQGFRYAYHTLHIRYLLLLLGAGT
                     LAGVPALVLMPFFANDILGKGSWGLGILLAGMGIGALIGTLVLAGRSTSDGLVRVILV
                     GSFGLAASLIALAVSRNFYLSVAVMVVLGFSTLRQMAATNTLIQSLIPDHYRGRIMAL
                     YTVTVVGLGPFGSLLSGAVAHRFGPPATVAAGGVFCLAAGLLFRARLHQFQRSILEVN
                     Q"
     gene            complement(3317..>4626)
                     /locus_tag="VFA54_16820"
     CDS             complement(3317..>4626)
                     /locus_tag="VFA54_16820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS52533.1"
                     /translation="RAELALGLLFAINLLNFYDRHILGAVTEPIRREWGLTDTQLGWL
                     GAAFILLYAAVGVPFGRLADRFSRKALLTAGLFFWSVLTALSGAAAGFWSLFATRLGM
                     GIGEAVCAPVASSLIGDLYPASRRSWAMSVFMLGLPAGAALSFFGTGAIAYSFGWRAA
                     FFVAGAPGCILALLIRKLLADPPRGAAETVNVGARRRAGSAFLVVLKIPTMWWIIISG
                     ALHNFNMYALAQFLPSFLIRSHGLDLRRAGFVSGVIFGAAGAAGLLLGGRLADYLARS
                     RANARLWLGAVCLFAAVPLSLAALGQQPGSPYRFLLWMLPGCALLYVYYSVVYSSIHD
                     VIEPSLRGTAMAIYFLAMYALGGAAGPVVTGKASDLLARRAARAAALSETARAAGLHQ
                     AMYLIPALELLTGVVLYLGSRTVVRDQRNLAEWMAGAAQPVVR"
BASE COUNT         1060 a         1432 c         1401 g          733 t
ORIGIN      
        1 cgtcgcgtaa attggcttcc tggtgggacc aaaaagcttg gtccatgccc ccgtgttcgg
       61 cgcggatgac gcccttacgg tcgatcatga aaacataagg cacaaagaaa ctctgtaccg
      121 ccgaaatatc ggcgaacgtc cgcaccatcg tcgggtcgcc caccccgatt ggataggtgg
      181 cggcgaagcg gcggcggaaa tcgtcaatca gatggatggc tccgggatta atggccacgg
      241 caagaatttc gagcccacgc gaacgccatt ctttatagat cggctcaata atgcccgccg
      301 tgtgctggca atgaggacag gtagtctgaa agaactcgag cagcacgact ttgccgcgca
      361 tactaggaag ggagtaggag tcttgactgc caagggcggc gccgggcggg gcacatcgcc
      421 ggcccgcaag agcgcggcga acaacaagaa tacagtcaac ggcgcaaact cgaatctcac
      481 ctggcgaggc atgtgaacaa caattctaga tacgggctct ccagaagttc aacccccgat
      541 cggcgccttg tcacaatcgg aacgcggcag ggttaagcag atcccgccaa aatgaagagg
      601 ggcgccgcgg cttagggcat accctctacg cttgtaaaaa ccgctccgtg ctgcgaccag
      661 gcgaagcgtc cgtcgtcgcc ggctccggtc gcctgatggc cttcactgcc ggatacggtc
      721 atgtgcttcc atttcaggcc gcaaaccttg cggtaatcct gaaaatagcg gctggcgtcg
      781 gcgtcgctcg accactcgac cgcgtagccc agcacgtccc ggtgtccttt tttgtcctcc
      841 caaaggacgt agcggcttcc cctccatttc ggagccaggt cgcgggcttc ctcttcgccc
      901 aggaattgcc ggagcagaat ctggaaatcc agttccccca tcgttccctc ggcgattttc
      961 ttgtatccgg ggggcaattc tgcccgcggc aaagccggct tgctgggcat ggagcgggca
     1021 aaatagcgct ctggatggag cacctgctgc gtactcaccg gtgcgtgctt gaaaacttcc
     1081 gtgaatgcct cgcggcccaa tttctggatc acagcctgtt gaaacagcat tccctgggtg
     1141 tagggaaaca tcaaagtctc ctggaggtaa agcggagact tgccgaaaac gggatattcc
     1201 ttggcgccct cggcggtcgc ttcgagcgcg gaagcggcaa gctgcggagc atctcggagc
     1261 gattttccca tttgccggag catgtactcc gtcatcaccc aggaggcctg gccctcgacc
     1321 acggcgccgc gggcggtgga tgcgtcatcg tcgtcgccgc tcttttcaat aaacttctcc
     1381 aggttgaagt gctgatcggc aagggcgtgg gccagctcgt gcaccagcgc aacgtcttgc
     1441 atgcccgagg gggtccagga ggcgagatag agcttctttt ccttgaaatc gtaaaatgcc
     1501 gccgcctgct cggtaagcag atcgaccgtc tgggccttca gatcgtagtc ctgcggaatg
     1561 aagccgaatt tttttaggac gatctcttcc acccgcaagt cttcaggcgg cagcgtttcg
     1621 ctcatgcgct gctcgatgta ttcccggatt ttttcccggc tgatgactgc gcgtggaacc
     1681 ttccggcgga tcttgaggcc catgatttgg ctggtttcat tgaagatttc atcgatgcgg
     1741 gagaaaagct gcgcttcctg ccccgcggta atttgcgtgt cagcggcggc ttggggagcg
     1801 ctcgccgctg gccggtggat gagcgcggcc agaagtacag caagcaaaag ccacggcagc
     1861 gcgagccgct ttttcattga ttcacctcga gaatcgatcg ttggaactga tgcagccgcg
     1921 cgcgaaatag taagccggcg gcaaggcaga acacaccgcc cgcggccacc gtggccggcg
     1981 ggccgaagcg gtgagccaca gcgccggaga gcaagctgcc aaacgggcca agcccgacca
     2041 cggtcaccgt atagagcgcc atgatccggc cgcggtagtg atcagggatc aggctttgaa
     2101 tcaacgtatt cgtggccgcc atctgccgca gcgtgctgaa gcccagcacc accatcaccg
     2161 caacggagag atagaaattt cgggagacgg ccagcgcgat caggctcgcc gccaatccaa
     2221 acgagcccac caagattacc cgaaccaggc catcgctggt gctgcggccg gccagtacga
     2281 gcgtgccgat caaggccccg attcccatgc cggcgagcag aatgccaaga ccccacgagc
     2341 cctttcccag gatgtcattg gcgaaaaacg gcatcagcac cagagcgggc acgcccgcca
     2401 gcgtgccggc gcctaacagc agaagcaggt accgaatgtg cagcgtgtgg taagcgtagc
     2461 ggaacccctg gatcagatga tcccagggcg agtcggccac agagcgctca aacggctgca
     2521 agcgcatcag caacaggctg ataatgacgg cgataaagct ggcgccgttc aggagaaagc
     2581 aaacgccctc gccgagccag cctaccagca ccccggcaat gccgggaccg accacgcggg
     2641 ccgcgttgaa catcgaagaa ttcaaagaga tggcgttcag caaatcctcc ttgctggtca
     2701 tttcgacgat gaaggcttgc cgcgccggca tatcgaacgc attgatgcat cccagcgcta
     2761 cggcaagggc gagtacctgc cagacctgca cgcggccgga gacagtgaga gcgccgagca
     2821 ggaaggcctg gaccatggag agcgtttgcg tggcaatcac aatccggtgg cgcgatctgc
     2881 ggtctgagac gatgccgccc aagggcgcca aggcaaagac ggggatttgc aggcaaaaat
     2941 acgtgaggcc caccagaaac tcggagcgcg tcaggcggta aatcagccag gtctgggcga
     3001 cattctgcat ccaagtgccg cagagtgaaa tgatctggcc gaaacagaac agccggaagt
     3061 tccggtggcg cagagcgcga aagacctggc gaaccccctt ctgcgggagc accgcggctt
     3121 cgcccgaaac gccttccgca acacccgaag ggacggcggg gccgctggcc agttccgcgc
     3181 cgccccccag cgcggcggac agttcctgct caccagtgga ttcgcccatg aagccagaac
     3241 tccaggataa agcatcaccg ccacaacgca acccctcagt cggagcaagc ccgcgcagac
     3301 cgcagccgga gaggtctcag cgcacaaccg gctgcgccgc gccggccatc cattcggcga
     3361 gattgcgttg atcgcggacg actgtgcggg aaccgagata caacacgaca ccggtaagca
     3421 gttcgagcgc ggggatgagg tacatagcct ggtgcaagcc ggcggcccgc gccgtttcag
     3481 acagcgccgc ggcgcgtgcg gcgcgtcgag ccagcaagtc gctggccttg cccgtaacga
     3541 ccggcccggc ggcgccgccg agcgcgtaca tggccaggaa ataaatagcc atggcagtgc
     3601 cgcgaagcga tggctcaatc acgtcgtgaa tggatgagta aaccacggag taatacacgt
     3661 acagaagcgc gcagcccggc agcatccaca agagaaatcg atagggactg ccgggctgct
     3721 gacccagggc ggccaggcta agcggaacgg cggcaaacaa acagactgca ccgagccaca
     3781 ggcgcgcatt cgcccgggag cgggcgagat aatcagccaa acgccctccc aaaagcagtc
     3841 ccgcggcgcc cgccgcgccg aaaatgacgc ccgagacgaa gccggcccgc cggaggtcca
     3901 gcccgtgaga gcgaatcaga aaagacggca aaaactgcgc aagcgcgtac atgttgaagt
     3961 tgtggagcgc gccggagatt atgatccacc acatggttgg aattttcaga accaccaaaa
     4021 acgcagatcc ggcccggcgc cgggcgccca cattcacagt ttcggcagcc cctcttggcg
     4081 ggtcggcgag gagcttcctt atcaaaagcg ccagaatgca ccccggcgcg cctgccacaa
     4141 agaatgccgc ccgccatccg aagctgtagg caatggctcc cgtgccgaag aaactcagcg
     4201 cggcgccggc aggcaagccc agcatgaaga ccgacattgc ccaggagcgc ctcgaggcgg
     4261 gatacagatc gccgatcagc gacgacgcaa cgggcgcgca aacggcctcc ccgatcccca
     4321 tccctaagcg ggttgcaaac agggaccaaa agccagcggc ggctccggaa agagcggtca
     4381 gcacgctcca aaagaacagc ccggcggtca acaacgcttt cctgctgaac cggtcggcaa
     4441 gacgtccgaa tggcactccg accgcggcat aaagaagaat aaaagccgcg cccagccagc
     4501 ccagttgtgt atcggtaaga ccccactcgc ggcggatagg ttcggttacg gcgcccagaa
     4561 tatggcgatc ataaaaattc aaaagattga tcgcaaacag cagcccgagc gcaagctcag
     4621 cgcgta
//
LOCUS       DASREK010000312         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2297189, whole genome shotgun sequence.
ACCESSION   DASREK010000312 DASREK010000000
VERSION     DASREK010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297189"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..777
                     /locus_tag="VFA54_16825"
     CDS             145..777
                     /locus_tag="VFA54_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZS52534.1"
                     /translation="MADAHVEEVMATEWGGGAAPYAVNSKKLGMWLFIISDALTFSAL
                     LVAYSYVRMANKNWPTPFEFGAMANATIMTFVLLSSSVTMVLAVAAAHKGLRATAAKW
                     IFATMAGGILFDILHSREWLHLFEEGVTPFGNPWGVPLFGASFFTLTGLHMLHVTCGV
                     IYLAFVAIGYGMRGKFADIDVEVAGLYWHFVDLVWMFIFPLVYLMSNKMI"
     gene            793..1104
                     /locus_tag="VFA54_16830"
     CDS             793..1104
                     /locus_tag="VFA54_16830"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HZS52535.1"
                     /translation="MDATVEHAHSGNRLYFLVWFWLLALTLLEVFLAYRQLPLMTMLF
                     LLMGLSLIKAALIIAYFMHLRFEKRSLALTLIPPLVICVILLMVFFFPDSLRLLELRP
                     S"
     gene            1104..1382
                     /locus_tag="VFA54_16835"
     CDS             1104..1382
                     /locus_tag="VFA54_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52536.1"
                     /translation="MTSCGWRLRDCGQPGTATRRRGKLGLLAAAWSTATLAAGAAFGQ
                     CAMCYATAASQGPQARHALNLGILLLILPVASIIGGIAVVTYRNRNSE"
     gene            complement(1441..2586)
                     /locus_tag="VFA54_16840"
     CDS             complement(1441..2586)
                     /locus_tag="VFA54_16840"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HZS52537.1"
                     /translation="MQLALWRNFSAAACLCLWGLFPCGAMAGSLGAWSNTAAMASARQ
                     AHTATLLAAGGSVLVAGGFQPDTNSYLSSAEIYVPGKGKSDRWVPAAGMNVARAGHTA
                     TVLRDGRVLVTGGFDGTNVLASAEIYDAAANTWTTAGSMNTARWGHTASLLGDGTVLV
                     VGGVDNTGHSLASAEIYDPAGNTWSYTSSAPVFARSWHTATLLNDGSGGVLLAGGLDG
                     GGVAQATVEIYDPLNGFFQVGSLASARAGHTATLIGANDQILVVGGHDETGADLATAE
                     VFDGVTGNWAGTGSLGTPRESHTATALQNGKVLVAGGASNQAPLSSSEVYDPSAGTWS
                     SNRNIQLDAARGGHTATLLNDGNVLAAGGQGVSGALSSAELYKAPSH"
     gene            complement(2971..3912)
                     /locus_tag="VFA54_16845"
     CDS             complement(2971..3912)
                     /locus_tag="VFA54_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS52538.1"
                     /translation="MTAPSDIGFAIEVQSLNYFYGERRALDGVSFAVATGEIFGLLGP
                     NGGGKTTLFHILATLLIPASGSARIFGLDVAVEPQAVRRRIGVVFQAASLDKKLTALE
                     NLIHQGHLYGLHGQALRGRALAALERVGLADRAQDRVETLSGGQQRRVELAKGMLHGP
                     RLLLLDEPSTGLDPGGRLDLWRYLTYLRDSEGVTVLVTTHLLEEAERCGRIAILDRGR
                     LVTQGAPEELKKRIGGDVIVVGARDPESLRRQVEDRFHLPATTVDGVVRIERERGHEF
                     VAQLVEAFPGQIDSISVSKPTLEDVFIQQTGHKLWDG"
     gene            complement(3918..>4723)
                     /gene="cyoE"
                     /locus_tag="VFA54_16850"
     CDS             complement(3918..>4723)
                     /gene="cyoE"
                     /locus_tag="VFA54_16850"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZS52539.1"
                     /translation="TWLILMSTGVGFYIGSAHPFGALLLLFHALLGTALVASGTAALN
                     QWMERDVDARMHRTRQRPLPAGRLEPSRAFWFGLVLSIAGTVYLAAAVNLLTGFLGAF
                     TCASYLLCYTPLKKRTTLSTFVGAFPGAMPILMGFAAARGRLTLEGWVLYGILFLWQF
                     PHFLSIASLYREDYERGGIVMLPVVDREGVATRRQIIAYTAALLVVSAIPAFLGFSGP
                     VYLFGTLLLGGVFLYFGADMRTRLQARRLLQASVFYLPLVYGLMVLDKQ"
BASE COUNT          955 a         1449 c         1429 g          890 t
ORIGIN      
        1 tctggcgggc gtggcgcagc cgcaaccccg agagattctg gcgcacccgg caagcagcta
       61 ttgaatccac ggccatctat tggcatttta tggacggctt gtgggtctat ttaattttgt
      121 tgctatcggt ggggaggtaa aggaatggct gacgcacacg tcgaagaggt gatggcaacc
      181 gaatggggcg gcggagccgc gccttatgcc gtgaattcga agaagctagg catgtggttg
      241 ttcatcatct ccgacgcatt gacgttttcc gcgctgctgg tggcttacag ctacgtgcgc
      301 atggcgaaca agaattggcc cactccgttt gagtttggcg ctatggccaa cgctacgatc
      361 atgaccttcg tgctgctgtc atcgagcgtg acgatggttc tggccgttgc ggcggctcat
      421 aagggcttgc gggccacggc ggccaaatgg atattcgcaa cgatggccgg cggcattttg
      481 ttcgacatcc tgcacagccg ggaatggctg cacctgtttg aagaaggcgt gaccccgttc
      541 ggcaatccct ggggggtgcc gctgtttgga gcgtcatttt tcacgctcac cggccttcac
      601 atgctgcacg tgacctgcgg cgtcatctat ctggcgttcg tggccatcgg ctacggcatg
      661 cgcggcaaat tcgccgatat cgacgttgaa gtggccggcc tgtactggca tttcgtagat
      721 ctggtgtgga tgttcatctt ccccctggtc tacttgatgt ccaacaagat gatttaagga
      781 aaacggaaat ctatggacgc aacagtagaa cacgcgcaca gcggaaacag actgtatttt
      841 ctggtctggt tttggctcct ggccttgacg cttctggagg tgtttctcgc ctaccggcag
      901 cttcccctca tgaccatgct gttcctgctc atgggcctct ctctcatcaa agcggcgctc
      961 attatcgctt actttatgca cctgcgcttc gaaaagcgca gcctcgccct gacgctgatc
     1021 ccgcccctgg tgatctgcgt cattctgctg atggtgttct tcttccccga cagcctgcgg
     1081 ctgctcgaat tgaggccaag ctgatgacga gctgcggatg gcggctgcga gactgcggcc
     1141 agccggggac ggcaacccgg cggcgcggca aattgggtct tcttgctgcc gcttggagca
     1201 cggccacgct cgccgctggc gcagcgtttg gccagtgcgc tatgtgctac gctaccgccg
     1261 catcgcaggg cccgcaggcg cgccacgcat tgaatttggg cattctgctc ctgatcctgc
     1321 ccgtcgcctc cattatcggc ggcatcgccg tcgtaaccta ccgcaatcga aactccgaat
     1381 aaaaccgcag ccactggtcc ggatgcccgg cggacgagga gcagcggtgc gtatcggcct
     1441 ttagtgagag ggagctttgt aaagttccgc agaggagagc gcgccggaaa caccttgccc
     1501 tccggccgcc agcacgttgc catcgttgag cagagtcgcc gtgtggccgc cgcgcgccgc
     1561 atccaactgg atattcctgt tgctgctcca ggttcccgcc gagggatcat acacttccga
     1621 tgaagaaaga ggcgcctgat tgctggcgcc gccggcgacg agtactttcc cgttctggag
     1681 ggcggtggcc gtgtgcgact ctcgcggcgt tcccaggctg cccgtgccgg cccaattgcc
     1741 ggtgacgcca tcgaatactt ctgccgtcgc caggtcggct ccggtttcgt cgtggcctcc
     1801 gacgaccaga atttgatcgt tggcgccgat caatgtagcg gtgtggccgg cgcgcgcaga
     1861 cgccaaactg cccacctgaa aaaatccgtt gagcggatcg taaatctcca ccgtcgcctg
     1921 ggccacgccg ccaccatcca gaccgccggc cagcaaaact ccgccgcttc cgtcgttaag
     1981 aagagtggct gtgtgccagg accgggcgaa aaccggagca gaggaagtgt agctccacgt
     2041 attgccggcg gggtcgtaaa tctcggctga ggccagactg tgacccgtat tatcgacgcc
     2101 tcccacaaca agcacagtgc cgtcgccgag cagtgacgcc gtgtgccccc atcgggcagt
     2161 attcatgctg cccgcggtcg tccaggtatt ggcagcggcg tcgtaaattt cggcagaggc
     2221 aagcacattg gttccatcga acccgcccgt aaccagcacc cggccgtctc ggagcacggt
     2281 ggcggtgtgt cctgccctgg ctacattcat gccagcggct ggaacccacc ggtcgctctt
     2341 gcccttgccg ggcacgtaga tctctgcgga agagagatac gagttcgtgt ccggctggaa
     2401 cccgccggcg accaaaacgg agccgcctgc cgcaagcagc gtggctgtgt gcgcctggcg
     2461 cgccgaggcc atggccgcgg tatttgacca agcccccagg gatccggcca tcgccccaca
     2521 aggaaatagc ccccacaggc agaggcacgc cgctgctgaa aaattgcgcc atagagctaa
     2581 ttgcatcact gacctccaaa gcaagaaaaa ggcctcggcc ctaagggccg atgcacattg
     2641 caaaaaattg tccgggggga gccgccagcc gggaaggttc atgcccgcca gccggcttac
     2701 caacggaagc aagcgcggcg gcctgcggca aaacgattat cgtttcaatc gaaagatagg
     2761 ttcaattgag taatttgctt aagcagcctt aagacaaagc cacgagatgg atggaatgga
     2821 aacgtcttag gagcgcgggc cggcgataag gcgtatcgcc acggaagcga ggcgctgtgc
     2881 gatatgaagc gcggtcggaa tggatcagga gcgccagttt ggatggctcg cagagccatg
     2941 cccacttccg tactttcgcc gcccgggata ttacccgtcc cagagcttgt ggccggtctg
     3001 ctggatgaaa acatcttcca gggtaggctt ggatacgctt atggaatcga tctggccggg
     3061 gaaggcttcc accaactgcg cgacgaattc gtggccccgc tcgcgttcga tgcgaaccac
     3121 cccatccacg gtggtcgccg gcagatgaaa gcggtcttcc acctgccggc gcaggctttc
     3181 cgggtcgcgg gcgcccacta cgattacatc gccgcctatg cgcttcttaa gctcttcggg
     3241 ggcgccttgt gttaccaggc gcccgcggtc aagaattgcg atccgcccac accgttcggc
     3301 ctcctcaagc aggtgcgtgg tcactaacac cgtgacgccc tcggaatcgc gcaggtaggt
     3361 gaggtatctc cacagatcca gccgcccgcc tgggtcgagc cccgtcgagg gctcatcgag
     3421 gagcaacagg cgggggccgt gcaacatacc ttttgccaac tcaacgcgcc gctgctggcc
     3481 gccggaaagg gtctctaccc ggtcctgggc ccggtcagcc agccctacac gttccagagc
     3541 cgccagagcg cgcccccgga gagcttggcc gtgcagcccg tagagatgcc cttgatgaat
     3601 caagttctcc agggccgtga gctttttgtc aaggctcgcc gcctgaaaaa cgacgccgat
     3661 ccgccggcgg actgcctgcg gctcgaccgc tacgtccaga ccaaatatcc gcgccgagcc
     3721 cgaagcggga atgagcagcg tggcaagaat atggaagaga gtggttttcc ccccgccgtt
     3781 gggccccaga agaccaaaaa tttcacccgt cgcgacggcg aagcttaccc catccaaagc
     3841 ccgcctttcg ccgtaaaaat aattcaggct ctgcacctcg atggcgaaac cgatgtcgga
     3901 cggcgcagtc acgttggcta ctgcttatcg agaaccatca agccgtagac cagcggcaaa
     3961 taaaataccg aggcctggag caggcggcgg gcctgaagcc gcgttctcat gtcggcgcca
     4021 aagtaaagaa aaacgcctcc cagaagaagg gttccgaaaa gataaaccgg tccagaaaag
     4081 ccgaggaaag cgggtatggc gctcaccacc agcaacgcgg ctgtgtacgc gataatctgg
     4141 cgccgggtag ctacgccttc ccggtctacc acgggcagca tcacgatgcc gccgcgctca
     4201 taatcttccc ggtataggct ggcgatggag agaaaatgcg gaaactgcca gagaaacaga
     4261 atgccgtaga ggacccagcc ttccagcgtt agccgcccgc gtgcggccgc gaagcccatc
     4321 aaaatcggca tcgcgccagg aaacgcgccg acgaaggtgg agagcgtcgt gcgcttcttc
     4381 agaggcgtat agcacagcag gtagctggcg caggtaaaag ctccgagaaa gccggttagc
     4441 aggtttacgg ccgcggccag gtaaaccgtt ccggcaatcg acagaaccaa gccgaaccag
     4501 aacgcccgtg agggctcgag ccggccggcg ggcagcggcc gctgccgggt gcgatgcatt
     4561 cgggcatcca catcgcgctc catccactga ttgagcgctg ccgtgccgct ggccaccaga
     4621 gctgtcccga gcaacgcatg gaacaggagc agcagtgcgc cgaatggatg cgcggatcca
     4681 atgtaaaatc cgacgcccgt gctcatcagg atgagccagg tga
//
LOCUS       DASREK010000313        63172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1137353, whole genome shotgun sequence.
ACCESSION   DASREK010000313 DASREK010000000
VERSION     DASREK010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 63172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63172
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137353"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFA54_16855"
     CDS             complement(<1..917)
                     /locus_tag="VFA54_16855"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZS52540.1"
                     /translation="MSAGPTTYVEAIRQGIWEEMERDDSVFVIGEDVGAYGGAFKVTA
                     GLLDRFGERRVIDAPLSEAAIVAAGTGAAIAGLRPVVEMQFADFISCAYDQIVNFAAK
                     SRYRWGAGVPMVIRGPSGGGIHGGPFHSQNPEMPFVHTPGLKVVAPATAYDAKGLIKA
                     AIRDNDPVLFFEHKALYRKVKEDLPAGDYVVPLGKAAIRRAGQDISVITYGAMVHVAL
                     EAAATLAGEGIDLEIVDLRTLAPLDEAAVLETVRKTGKVILLHEDTMTGGLAGELAAR
                     ISEKAFDYLDGPIVRITAPDCPVPYSPPL"
     gene            complement(934..1923)
                     /locus_tag="VFA54_16860"
     CDS             complement(934..1923)
                     /locus_tag="VFA54_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HZS52541.1"
                     /translation="MMTTATPVAPEVTQDLCSRMLYYLELMREIENRIELKLYRQGKI
                     LGGCYTGRGQEAIPVGSAILAEKGDWICPTHRDMGAFLVRGMESKRIFAQYLGRATGP
                     TRGRDGNMHMGDLDLHILPIISSIGASVPVAGGIALAMRYRGQPNIVFSYFGDGATSR
                     GDWHEGVNFAAVQKLPVVYLCNNNQFAYSTPLEKQMAVPNVADRASAYGIPAEIVDGN
                     DVIAVHQAARRAVAHARGGRGPYMIECKTFRMTGHSAHDAAEYVPKAKFEEWSKKDPI
                     LMLQKYMVERNWATEVDFKRLHARILAEVDEAVAWAEQSPMPDPSTLLDGVYE"
     gene            2059..2409
                     /locus_tag="VFA54_16865"
     CDS             2059..2409
                     /locus_tag="VFA54_16865"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HZS52542.1"
                     /translation="MATAPNAAAYCRRCGVMFQAEDRYCRNCGYDSAAPLGASVPTGT
                     PAEASSRRRSAAALLCFFLGVFGAHRFYAGRIGTGLLWLFTFGLLTFGMLFDLILILA
                     GEFRDAEGRKILVW"
     gene            complement(2422..3810)
                     /locus_tag="VFA54_16870"
     CDS             complement(2422..3810)
                     /locus_tag="VFA54_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS52543.1"
                     /translation="MPQPKLLRELGVFSATAIVVSNMIGTGIFTTTGFLAGDLGSAWL
                     VVAIWFAGAVLALAGALCYSELGINFPRSGGEYVYLTEAWGPGWGFLTGWVSFFAGFS
                     APIAAGALAVAGYLAYFFPALKAGGSGSIQAAFLTVHLGPAQLVACAVVLAFTTLNVF
                     GLLRAARIQNLLTTLKIITIVCFIVLGASIGHGDWGHFMAAAQRTSPHSLGVQFLISL
                     VWVFFGYSGWNAATYVAGELRDPERTLPRSLLAGTALVAALYAGLNIVFIYGAPLDAM
                     KGVVGVGSLAARSLFGPAVAGAFSALMALALLSTVNAMTIIGPRVYYAMAENGAFFRA
                     AERIHPRWNTPWVAIWAQGACCCVLIITGTFESLVQYVGFSLWLFTMLAVLGVFRLRR
                     RPGWKSLPAANVAFPLIPGVYVAISAWVLIYMVLLRPKESGLGLLTMAGGALIYLLVL
                     RRKPAVRRAATL"
     gene            complement(3929..4789)
                     /locus_tag="VFA54_16875"
     CDS             complement(3929..4789)
                     /locus_tag="VFA54_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HZS52544.1"
                     /translation="MPFCTSCGFKLPEGSAFCANCGSPAGGSAGASGQVRVLPLEYSI
                     EGDNLQVARIKLKPGQELYAEAGKMVYKTANVEWQTRMSGQTIGQKLMGVLRRTVTGE
                     SLFLTYFRTLGAEGEVGFAGDYPGKLEAIELAAGQSVLVQRDSFVCAEASVAFGIQFT
                     RRLGAGFFGGEGFILEKFTGPGTVFIHGGGDFVHFNLGPGQVLQVDTGCIVGFDEGVD
                     YDIQLAGGIKTAIFGGEGLFLATLRGPGKAIVQSMTLSKLRRELGGGRRGGDEQHGLE
                     TIADLFSSDD"
     gene            complement(4935..5120)
                     /locus_tag="VFA54_16880"
     CDS             complement(4935..5120)
                     /locus_tag="VFA54_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZS52545.1"
                     /translation="MAQITKALVIARLWTDTRGQDLVEYALMAGFITVAIGAAFPPVS
                     SSISTIFSRVGSLLSQA"
     gene            complement(5320..7266)
                     /locus_tag="VFA54_16885"
     CDS             complement(5320..7266)
                     /locus_tag="VFA54_16885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZS52546.1"
                     /translation="MLQQIGRYRVLSELGRGAMGVVYKAQDPTIGRLVAIKTIRLGDL
                     AHPEEREQLRSRLFREAQSAGILSHPGIVTIYDIAEESDQAYITMEFVEGQTLEKMLD
                     AGLVGDAKYLVSIATQTAQALDYAHSKGIVHRDIKPGNIMVNTEGAVKITDFGIARIA
                     SSKFTHTGTVMGTPSYMSPEQVRGAVVDGRSDEFSLAVVLYEMLTGQKPFLGDSITTV
                     IFKIVSESPVPPKEINPSIGSKVNAVVMKALAKEPAERFASCRDFAEALQLAVAQTAN
                     LTPTLRKMPSLFATTEASDPAVMTQPDKPASRAEDLPTVVEMAGSPPLPAAPLGPAAA
                     PSEAIHFPEAAVNEAAAAAEETPPQSLAGSQSAAAPSAIEPGPGPERAEKLPPLEVRD
                     ALAPRAGLPPPPSEPVSRGRGVLLFAVAALLAAVGGEIFWQMHRAQPAQEKPSMSVEP
                     AGTAPTPPNPPAPASNLASAPPAAAAPQDASTAPEKPIPIEKSGAERSGSNASKTAAG
                     GGVVNISTSEPGARAVVDGDDLTACITPCSIPVKAGSHTLLLSQAGFQTERREITVGN
                     RPIDVQITMHPILGTVMLSTVPPGATITVDGKPVEGETPKALKLPPGRHVITASKEGV
                     GAAEQTIEITDDSLHSVRMTLQNP"
     gene            complement(7431..8789)
                     /locus_tag="VFA54_16890"
     CDS             complement(7431..8789)
                     /locus_tag="VFA54_16890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZS52547.1"
                     /translation="MKITVVGTGHVGLVVGLCFAEIGHEVGCHDTDTAKIEALARGVT
                     PFYEPHMDRLLQRNSERGRIRFFSDLEPHINDADVIFLCLGTPPLPNGEADLSSVEKV
                     TRQIATHSRSYKLIVEKSTVPVLTGKRIHATMQIYGGSAPFDVASNPEFLSEGAAIHD
                     FLHPQRTVIGVESERAEALLREIYDPILRQSFDCPIHEGCEWKGRSTPLVVTNRNSAE
                     IIKHASNSFLATKISFINAIATLCEKVGADVEQVAAGMGLDSRIGPSFLRAGIGFGGF
                     CFPKDLQAFCHIAEKNGYDFRLLREVERINQLQMEHFFQKIKNEVWVLKGKRIGAWGL
                     AFKPDTDDVRFAPALAVIEKLLAEGATVAAYDPRAIKEACAALPSASHRIVYCQSAEQ
                     AASGADALVIFTEWQEFLCADFARIRESMARPLIIDGRNMFRPEEMRRRGFEYISVGR
                     PQ"
     gene            complement(9001..10557)
                     /locus_tag="VFA54_16895"
     CDS             complement(9001..10557)
                     /locus_tag="VFA54_16895"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS52548.1"
                     /translation="MTGELRVRTRRRLALLVYWMPLLLSSILGAESLGTISGIVTNAD
                     TGAALAGSAVVLTQADGSPVRTVLTDRQGRYVFDQLPPGYYLVKASRANFATGRYGQK
                     SWDRVGRPIALGAGAGFSAEIRLHRLGAITGWVVDENGEGIPDFTVNAITAEPHAISG
                     HVLFSAVTDDRGYFRIPGLQPGRYYVATAPRELPDGTGLLPTYYPKTLEMAEARVVNV
                     TIGRETENIQIQPIAGALLHFSGIVGGAPRFPHSRANITLFRDEEKRETTAGAFGQFA
                     FGGLVPGRYTLVAELDAPQGKLCAYQPLEIHGDQEGYTLSLTPAPQVSVRVVDEKGVE
                     QNDPQILVFLSRVENGARTQPIRLEFRQGVYSSPGLMPGGWRFFVICPESAVVDDITL
                     DGKEALSGFVLPPNRRASAVIRIRRDAGRVHGRVTDSSGQPARGVSIICYPLDPQNRY
                     RLGGLRSQKTNLLGEYRFGGLPEGDYLIFSTEVEDFQPDDQIDALKAEIASVHVTRGA
                     NLVYDARVRE"
     gene            complement(10554..11408)
                     /locus_tag="VFA54_16900"
     CDS             complement(10554..11408)
                     /locus_tag="VFA54_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52549.1"
                     /translation="MSTHIFRQIRGALQNLNPREVQQMADQPFTIGLLAEDEPGYHRM
                     VQFLLPTGISEEKLRQASAHLLRIQTAADFEQCDLGLAEPHLEHPGRSFYGFDAAHPT
                     AAVERCLEEREDLWIPLARHFLPFRGPVIERLIRKISRENALFALASALPNIVPSFIE
                     LPWAVGEFASDIAVLTMNQIRMAFLIAAASDAPVGFIDQRGQIASIITSAFGWRAIAR
                     ELVSKVPFGCGLIPKGAIAFAGTYVVGLGLERYLRLGSGFTAQEKREEYQEALDRGRM
                     VVRQIVGS"
     gene            complement(11432..12967)
                     /locus_tag="VFA54_16905"
     CDS             complement(11432..12967)
                     /locus_tag="VFA54_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52550.1"
                     /translation="MSLRRKAARADPPEALHARIEEFVRACRSPAIFEPGEKTIPLEP
                     GRFDLQIRGSAVLLHVWSAETSLVRRILRVGREENGRIQLVTRRLAQGEGEMLVLDLA
                     RVGAEIERRAGRLEFRERFRRMLARQFGGWTIAQISAAPDLEHSLSPAYVRAFLTKGT
                     SALAAIAADPEAGAGSTRAAVCDQILSFGLIWLHYLRHRERRRTVEGLKIFLPQGRTA
                     ATSSRLAFLDRERASCELFAFDSQGFTSPLDEKNYGNLATSLDPASPVPQPAGPVAQW
                     IRRLIDEFGAEAVARPDGVLSIRLRGLEFARAMERQMTYGVGDERPVTGGNFYEVEAL
                     ARRIAEERRADAAPRESAFYHMAPEKWLESMVRADPAAIDGSLLPEPLYTQVPAVAGS
                     ERGVIDLLGSERSGRLVVVEIKASEDVHLPLQGLDYWMRVKWHLDRGEFQKRGYFPGL
                     AIQPTPPRLMLVSPVFHFHPTTETILQYFSQQVEVERIGLSADWRRSLRVVFRAKGAE
                     KPA"
     gene            complement(13346..13891)
                     /locus_tag="VFA54_16910"
     CDS             complement(13346..13891)
                     /locus_tag="VFA54_16910"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZS52551.1"
                     /translation="MNWLDVLIAAVIVISFVTSIIKGMTRELVGLVAVVTGILGALWW
                     YPLPARHIEPYAKNMEVASFAGFVLIFCAFLFAGFLISKILASLVKATGVRWVDRLLG
                     AGFGLLRGMLIAAALVLAIVAFAPGKRPIESVAESRLAPTVLQCAKAIVALAPRRLKD
                     GFENGLERVRKVWRETPSDTV"
     gene            complement(13888..14787)
                     /locus_tag="VFA54_16915"
     CDS             complement(13888..14787)
                     /locus_tag="VFA54_16915"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZS52552.1"
                     /translation="MPLPIREVLLQTNLPGCQLVGRGKVRDVYRANGRLLIVATDRIS
                     AFDYILPTGIPRKGEVLTQLSIFWFDFLRDIVPTHFVTADVSQYPEPLPRYREQLDRR
                     SMLVVEADMAPIECVARGYLSGSGWKEYLKHGTVCGIPLPAGLKESDRLPEPIFTPST
                     KALTGHDENISFSEVEKRVGAELAAKLRDLTLQIYSKAADYALSRGIIIADTKFEFGH
                     AGGRLILGDEVLTPDSSRFWPLESYKPGGAQFSYDKQYVRDYLESIHWNKQPPAPPLP
                     DDVVSKTSEKYLEAYRALTGKAL"
     gene            14838..16190
                     /locus_tag="VFA54_16920"
     CDS             14838..16190
                     /locus_tag="VFA54_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HZS52553.1"
                     /translation="MKTIIEPFKIKTVEPIRRTSPAERQRYLEQAGYNLFRIPARNIL
                     IDLLTDSGTSAMSTGQWAAIMRGDESYAGSESFYRMQKVVQEIFGFRHVIPTHQGRAA
                     ERILFSVMCKPGDVVPSNTHFDTTRANIEFVGAKAVDLPIPEAADTQARLPFKGNMDV
                     EGLRRLIADVTPRRIPLILMTVTNNSAGGQPVSMENLRAASNLAREFQIPFYIDACRF
                     AENAYFIKLREPGYKDRTPKQIAQEMFSLADGCTFSAKKDAFANIGGFLATNSDSLAE
                     QEENLLILTEGFPTYGGLAGRDLDAIAVGLEEVLEEDYLNYRIASTGYLGRHIADHGV
                     PIVEPPGAHAVYIDAGRMLPHIPRSQFPAQALAVELYRHAGIRSVEIGSVMFGENARQ
                     ELVRLAIPRRVYTQSHIDYVVEAILEVYENRESIRGFEITQEARFLRHFTAQFRPVAP
                     "
     gene            complement(16248..18299)
                     /locus_tag="VFA54_16925"
     CDS             complement(16248..18299)
                     /locus_tag="VFA54_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52554.1"
                     /translation="MTARRSIASLWALLLAASPALLGHEGGVRNPKPTKAKADLKLSE
                     RKMAAAKKKLAAQGRYNCCVKPSCSLCARVNGSCNCAANVAKGLGACGECYGGWQAGR
                     GAIQGVNKKSVKLLPSEKQACPTPGEPPDELKEATDALLRAKQTLVAEKRYFCCIRGG
                     CDQCAHEADCPCGQDLASGTAPEYYKIAGVKPPGQAQTKGVCGDCVDGWQSGRGNFSG
                     IPLSEVTLANMSGRGDASMGTAAANYASGTSQMPKAAPMDMLHQSLGDWNLMVHGELF
                     AVESQQTGPRGRDKFFSTNWFMPMLSRRLGPGTLTFRAMLSLEPATITNERYPLIFQT
                     GETANGGIPIISGQHPHDLFMELAASYQIQLGERTVLHFYGGPRGEPALGPTAYPHRL
                     SASEDPIATLGHHDQDSTHISNNVATAGLSYGPVTIEASGFHGREPDEHRWGIEGGAI
                     DSFAARLTVAPSSRWTAQFSAGRINQREATHPLRPTLRTTASVMYVRPLATGHWATTL
                     LWGRNNDLTYTQQPNFTQFFAASRQPADAVAPAGQESGTAPFLQPPFLPPPRNHIVTA
                     PTRIPGQIYNSYLAESTLFFKNKHWIWGRVENVDKDSTLIYEEQLFVLLVDERRYARV
                     QAYTAGYEYELPRYAGWLSTGLGGQFTYFGTPDVLKPVYGNHPLGIQVFLRFRLAPAA
                     R"
     gene            complement(18296..19486)
                     /locus_tag="VFA54_16930"
     CDS             complement(18296..19486)
                     /locus_tag="VFA54_16930"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HZS52555.1"
                     /translation="MRFLFLYVCLLSVIGFAAQEPPHQTAGKYDVMLRLPPDGLYAQE
                     EMQIEFRVVDRSRVDPLLGAAPVVRARIHATVDMPAMPSMPKYEEIAHTEGIPGEYGV
                     HPTFSHGGEYRLQLAIQPPADEAFQVTFPLQVLDADDARKRKPGPRPFQLELKSNPKN
                     PKAGQPVELELVVRAREKPREIFSSFEVMHEKLMHLLILRSDLAHFAHEHPERQGDGS
                     FQLQYTFPAAGEYHIFADTAPAGAGEQVMMAKIRVGGESDRKFDITKASAGERSRVKT
                     CDGVTVELKAPETYLPAKKTEDIFFALKDAATGEAVADLEPYLGAMGHLMLVHQDSVT
                     FVHSHPDEREPRAGRDGTVPFLVRFPKAGIYRGWSQFKRKGRIITADFILEVRDAAEP
                     GAAQ"
     gene            complement(19505..21184)
                     /locus_tag="VFA54_16935"
     CDS             complement(19505..21184)
                     /locus_tag="VFA54_16935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HZS52556.1"
                     /translation="MSHISVDSSGNVYFADSNNQRIRRISPDGTINTVAGSGDAPSID
                     NFCQPTSSVGDGGPAAAAKLFNPADVTVGPNGVLIIADQQNNRIRQVAGGTITTIVGN
                     GTHNPFAPGVPAPNSPMDWPSAVAVDPSGVLYFAEMHSHRVGKVGPDGKLVVVAGGIQ
                     STPDGPKAFGYNGDGIAATKALLYKPAGIALDSAGNLYIADQGNHRIRKVTPDGTIST
                     IAGTGQAGYNGDNIAATNALLSSPCDVKVDSKGNIYIADTGNNRIRRVSPGGTITTVA
                     GDGQPGRGPDGVLATASSLNFPGGIAIDAGGNLYIVDWQNYLIRKVSFSSQPTISAGG
                     VVNGASFAPAPVPVAPGSIISIFGLNLAPTTLAATQTPLPAQLAGTSVTINGAPAPLF
                     FVSPGQINAQLPFETAPGTATAVVTNAAGSSNPLTFNVAASSVGIFQYLPSNRAVVQN
                     QDGSLNSPANPEARGNVIVVYLTGQGAVNPPVPTGEAAPLDHVSPAVLPWAANIGGKP
                     ANVLFLGLTPAFVGLAQANIQIPQDVTPGDQVVMFISVGGQAGNTATISVK"
     gene            21609..22355
                     /locus_tag="VFA54_16940"
     CDS             21609..22355
                     /locus_tag="VFA54_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52557.1"
                     /translation="MSGKKIVLVASSTEANEYSQDCWIQMLFATLPAPVAKHFVNLDV
                     ILRNETWPDGQAKYVPNGLRVVESILLREFPPEDIAVCYPDQMERFIGSDTRAVGLHA
                     HNPLGITFATDVYAHLAGPRTEPLNAAEFKRAILHPVLRQHKSHLKILVGGPGAWQIE
                     QTGMRQEWGIDCLVDGEAEHLVLELFRAAVRGEPLPAVVKGASPESPDQIPVTRHRST
                     LGVVEITRGWRARLPILRRRCAARPEHPAR"
     gene            22324..23277
                     /locus_tag="VFA54_16945"
     CDS             22324..23277
                     /locus_tag="VFA54_16945"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52558.1"
                     /translation="MRRGQSIPLDNILQNVRANVADGADTIMLTTEDVFLYEQGPRWQ
                     PNREALLRLFRAVAAEPGVQYIHLTHATMAPVVQDPGMIEAITPYTVGMSRKRHPAST
                     HPENRYASVIIGLETGSVRLFKKYMPGKAYPYRPEQWPDVMLKGMEILNRHNWFPFCT
                     WILGLPGETDADTRESLDLLHALKGAKWCVVPTLFTPLEDTRLASREAAKLPQLTDLQ
                     WEFFFTCWRYNLDFYRGAKKKILFGLGIPLYYYLLGRKLFGERVKYPLWRLAHVPERI
                     MRGRLYLDLDRPATRFSVPVEAPVPLTAATPPERVGLMSLQ"
     gene            23588..24562
                     /locus_tag="VFA54_16950"
     CDS             23588..24562
                     /locus_tag="VFA54_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52559.1"
                     /translation="MHLAFIYNGVFIAIIAHGLIGGSLIWDKILLRRPETRNLLSYVF
                     WLGALSILGLCLIPFGFTMPPIPIALVSFGAGLLHMASIFFYYEALKRGEASQTLAIM
                     GGFSPLATALIGAPILAQPLGNQLLGFALLTAGGFVMFFSERIDLKSIVPPILLASGL
                     FGLVNVLIKVVYDHTNFVSGYVGFTLGTAAGAFFLLVRRSWRKQIFEQSEEASPRSRF
                     WYLVNRFLSGIGSFLVYYAVSLAHPAVVDAIAGVRYAIIFIGAFLITRLKPSWLAEDF
                     SGRALAGKIAGTALVTAGLVLLGLQGGETNAVYLRRPDVVAWFQSRWA"
     gene            complement(24589..24939)
                     /locus_tag="VFA54_16955"
     CDS             complement(24589..24939)
                     /locus_tag="VFA54_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52560.1"
                     /translation="MLEPESAKRDGIGVKTSFGIRVAVGAFTDQVEAPMTRTLERLPP
                     SFQRTVFSGFRAGKTIFDLSLEWQLPYSEIEKIIRLGIDSLERPVPSPLWAIPRKRQQ
                     KKARAVVTPFPSSR"
     gene            25378..25563
                     /locus_tag="VFA54_16960"
     CDS             25378..25563
                     /locus_tag="VFA54_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZS52561.1"
                     /translation="MAKVIRCRDVGVDCDFEARGETEQEILRKCAEHAKSAHNMDSIP
                     PELEAKVRGAIRDEEAA"
     gene            complement(25643..26866)
                     /locus_tag="VFA54_16965"
     CDS             complement(25643..26866)
                     /locus_tag="VFA54_16965"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HZS52562.1"
                     /translation="MRRRDFLCLAAAAAAKMPAAKGSAKISRITITPIEGRFHKKVAM
                     NAYDQAPKGETYATHLLRVFTAEGVSGTGTLDYSSPDKAMLESLRNLIGADPLELYEI
                     RQGRILTVRNPALAKYRFLDSVLFDLVGQILNVPCHELIGPSAREQVEVYDGTLYFSD
                     VMIPEKGVGAVVAEAEEAVRSGYRGLKLKVGRCDKWMPGVAGVLRDIEVVNAVRRAVG
                     PEVKIMADANNGYRGRLQLAWRFLSETQKSNLFWIEEIFPEDPELYARLREKMQQAGM
                     KTLIADGENMRSADELRPYLKPRTIDVTQLDIRRAGFLGNREAAELAAENEGFCVPHN
                     WGSQIGGLMNLHFAKAVAAAITAEDDRSRCDAIIADGYEFRNGFYTLPKTPGLSLRVN
                     EAVYQTKYKFREIAV"
     gene            complement(27035..28207)
                     /locus_tag="VFA54_16970"
     CDS             complement(27035..28207)
                     /locus_tag="VFA54_16970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZS52563.1"
                     /translation="MKLAQRMQNLGTESAFDVLVKARRLEAEGRSVIHMEIGEPDFPT
                     PPHIVEAAKKALDEGWTHYGPSQGYPDLREEIAGMVSATRRVRVGPEHVCVVPGGKPI
                     LFFSMLALLDAGDEALYPNPGFPIYESMIRYLGAIPVPLPLVESRGFSLDLNMLSERL
                     SPRTKLLILNSPQNPTGGVIPEEDLREIAAMVRDRDLVVLSDEIYSRILYGEQHYSIA
                     SIEGMMEKTILLDGFSKTYAMTGWRIGYGVMPERLAQAVTRLMVNSNSCTASFTQRAA
                     LEALRGPQESVDRMVEEFRRRRDVIVAGLNRIPGFRCALPGGAFYAFPNITGTGMSSA
                     ELETHLLENAGVAGLSGTSFGSYGEGYLRFSYATSLANIEEALHRVDQAVRDLARV"
     gene            complement(28227..28529)
                     /locus_tag="VFA54_16975"
     CDS             complement(28227..28529)
                     /locus_tag="VFA54_16975"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZS52564.1"
                     /translation="MTLYRCTSCGKLAVTQPAFCGCGNRKWLPATVSGKGKIYSCTTL
                     YAAAEPFEKDLPFQIAIIELEGGVRLTGRILGARVDIGDDVALVEERGGVHFFSAA"
     gene            complement(28702..29859)
                     /locus_tag="VFA54_16980"
     CDS             complement(28702..29859)
                     /locus_tag="VFA54_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HZS52565.1"
                     /translation="MRPVSIIGVGKTAFGAFPDRSLLDLAVEAGSKCLKDGHVTPPQV
                     EAFYLGNFAGPSFVGQNHLAPYVASALGMDGIPATRIEDACASSGSAFFHACAAVAGG
                     MYDVVLAAGVEKMTSVPGTRVTQILASAGDLEAEVKAGATFPAMFAMIARRHMYQFGT
                     TRDQLATVAVKNHANGAKNPDAHMRKLITKEQALNGRMIADPLTIYDCSLVSDGAAAV
                     LICPAGRAPDFSSKPIRILGVAQASDRLALADKDDITTFRSVQRAGEKAFQMAGLRPR
                     DIHVAELHDCFTIAEIIALEDLGFVPRGEGGPASASGFTAPDGELPVNTSGGLKAKGH
                     PVGATGIGQLCDLVLQMRCEAGERQVRRAETGLAQNLGGSGATCVVTILGR"
     gene            29963..30556
                     /locus_tag="VFA54_16985"
     CDS             29963..30556
                     /locus_tag="VFA54_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS52566.1"
                     /translation="MNTAGAAVSETYPAFQRQGSMETDKVPRPADVLQAAEQDFIAVH
                     LRRVFLLIHRIVGNVADAQDLTQEAFVKALARRDQLKDEQKAAQWLGRIAFNTAIDFM
                     RQRKRVSFCELEAAPEPQVFETPERLVLRAEKQAYLADGLRLLSERERAALILRDVEG
                     LPAAEVAQHIGCSMATVRSHIANARTKFRKYIKGRRA"
     gene            30644..31210
                     /locus_tag="VFA54_16990"
     CDS             30644..31210
                     /locus_tag="VFA54_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52567.1"
                     /translation="MSTLFWKHPGEADLALFAGGELGLLARWRIEGHLHACGECRQAV
                     GEFFELRSRVMDLAELPQMDWNGLAAQIQRRVEQQRRMSSSFAPARRAWRPAWAPALA
                     VLLMLAAGAYITRRYRAPEPPLSGAASLNASAGAVELRLGDRQVLTLTNDAQAESQVN
                     WRVSAGAVSARYLDADTGNITVNNVYTQ"
     gene            31194..32360
                     /locus_tag="VFA54_16995"
     CDS             31194..32360
                     /locus_tag="VFA54_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52568.1"
                     /translation="MSIRNSIVYVSFACWFFAAAAQAQLGQRTDPASVIRWSLGGAPV
                     SIVSADLGDSRVQPRGGAMILDLHATLTLQNNSSQNIRGITLAVLAQEMTPGGKGSVA
                     VPSLNVPPGASFPVRINLRLLRPLPAPAGPLVEVSLDGLLFADFSFFGPNRLESRRTM
                     TVWEMEARRDREHLKTLLEKQGPAALQREMLASLSRQAARPHLDVQVERASGSRAISA
                     AVSALTGRNLNFAFLRIPDSPLDLLSGAAQVNGAEAASLRVDVANRSNRDVRYFEVGW
                     IVKDAAGEQFLAGSLPSSPAGLDLRPGGSAVALQDRAYRFSRGPESLPAIIGLTGFVS
                     QVEFSDGAIWIPSRKALTEQSLLGVIPVSAEEQRLSQLYRTKGLQALVEELRKF"
     gene            complement(32382..34052)
                     /locus_tag="VFA54_17000"
     CDS             complement(32382..34052)
                     /locus_tag="VFA54_17000"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZS52569.1"
                     /translation="MRPKARLLTIFLAISAIVTAQPPPPKLILVVSVDQMRFDYLTRF
                     EPLYKGGFKTLLSRGAIFTNALYRHANSETGPGHSVILSGRHPSHSGIVANGWYDPYL
                     KTMMNVVDDSTVDAVGGRGHGASPANFVGFTVGDKIKQKWPASHVVGISFKDRSAILP
                     SGPRADAAYWFENDCGCFITSTYYTRQTPAWLTKFNEQKLPDSYYQTPWTRLLSDESP
                     YLQYSREDNFPGEWDLKDTTFPHGYRGKPPEPAYYESLRRTPYADEVLLQAALEALAA
                     HNLGTDAAPDVFVIGFSASDYIGHAYGPFSQEAMDNYLRLDLVLDKLFHAVDARVGLN
                     NTLVILTADHGALPLVEWLQKQGNSEAKRVPTSLLLNAVRTAFRQQFPAAPELIAGME
                     GPNFHLNLDAIARSGLKRSEVEQTAIRALMATGAVAAVYTWADMISDKPSSADPALAL
                     LRNSFFAPRSPHLMVVAKQYHYISGNPGGTGHGTVYEYDRHVPIVWMGAGIKPGRYPA
                     PAGPEDIAPTLAKMLGIQYPVEPDSRLLNEILGEGVSATTAGRVAPAR"
     gene            34286..36028
                     /gene="sppA"
                     /locus_tag="VFA54_17005"
     CDS             34286..36028
                     /gene="sppA"
                     /locus_tag="VFA54_17005"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZS52570.1"
                     /translation="MAKFLLGLITGIFLCVFLAVLVVVAAASFSGRAPSVSQGSTLVL
                     NLGGEIVEHNPANVPSLLFQRGMKPTVKDIHDILQKAAVDRRVNAVVLKARGIGAGWG
                     KIQEIRADLEQFRNSQKPLIAFLETPGAREYYLATACDKIYVAPQGLLDVKGFRAESV
                     FFKDTLSKLGVQADWVHVGKYKNFAEPFTQDKMSDATREVINSILDTVLNDFVATVAK
                     GRHMTPEQVRAALDQGPFLPEEAVKAGLADGLRYEDQVFDEVKNLTKVSAIKKLRAES
                     YSRVSLESLGLSGGSRIAVVYAVGDILQGEPEVSPFGGGEAIGSDTMARVLRDVGEDK
                     NIKGVIVRIDSPGGDAFASDEIWRSMNLLHSKKPMVISMSDVAASGGYYMAMSGDPII
                     AYPGTYTGSIGVVFGKFNLHGLYDKLGVRKEILSRGKFADIDTDYRDFTPEEMQKVMS
                     GMNSVYADFVQKVAEARKRKVSDIEPIAEGRVWLGSQAKQNGLIDELGGFDRAITLIK
                     ERAKLRPDDKVTLVSYPPEKKLLDVLLSRLNGAGDEDTLTETIRRKLGWAAPWRALLK
                     GGMLKVAPYIVSVQ"
     gene            36025..38379
                     /locus_tag="VFA54_17010"
     CDS             36025..38379
                     /locus_tag="VFA54_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52571.1"
                     /translation="MSTWKKPIALWLLSLGAAAAFAAEVDLRRAVVVAPPQLSPRESR
                     AVAMLVEEVEKRTRIRWPVAASRPSGSVPVIWIERPSGAGPAEGYRIRVDDSAAAPAV
                     FISGNDARGLLFAVGYLLRSMHMSAEKVTVAAALHVSAAPKYPLRGHQLGYRPKTNSY
                     DGWSLPMWEQYIRDLAVFGANAIELIPPRSDDDAWSPHFPLPPMETMVGMSRLADEYG
                     LDVWIWYPAMDKDYADPKTVAFALYEWGEVFRKLPRIDAVFVPGGDPGHTEPKYLMAL
                     LEKQTQNLHRYHPKAQMWVSPQSFSQAWLDEFLGILRTQQPPWLAGVVFGPQIRMSLA
                     QLRAAVPARYPIRHYPDITHSRQSQYPVQDWDMAYAATEGREVINPRPLDEARIFRLL
                     QRYTIGFITYSEGCNDDVNKAVWSALGWNPEADVTDILRQYSRYFLGDRYADDFAQGL
                     LALERNWQGPLLANQNVETTLRQFQSMERAASPQDLLNWRFQQALYRAYYDAYIRARL
                     VAETGDEQAAIARLAEAPRTGSLSAMAEAEAILDRAAERRPFFALRDRVFMLAEALFQ
                     SIRMQLSVPLYKAIAVGRGANLDTIDMPLNDRLWLKARFAELRRMPEESDRLNGIDAI
                     VNWTNPGPGGFYDDLGDLARQPHLVRGPVFEQDPAFLQSSLVSFSYRPEYRKSWWTYA
                     ESLNDTPLEMRYRDLDPAAHYKVRVVYAGDSPNTRIRLVANGNVEVHPLLQKPSPVRP
                     IEFDIPAEATASGELVLQWRREPALGGNGRGCQVAEVWLIKKSQ"
     gene            complement(38419..40554)
                     /locus_tag="VFA54_17015"
     CDS             complement(38419..40554)
                     /locus_tag="VFA54_17015"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HZS52572.1"
                     /translation="MPDHSLRLTRRELFAIPAVWAFARKPAGAQSTFPGVAYRNYSRC
                     LPDYLRDLAARAYEARNREIAKLTTPQAIRERQRWARATFWRLVGGRPESTPLNARTV
                     GSFERPGYRVEKVVYESRPELHVSANLYIPTTGRPPYPGVLFQMGHTANGKAGDTYQR
                     CCQGLARLGYVVLAFDPMGQGERIYYPNASLTRTRLFSPDDEHTTAGRQMLLVGDTAS
                     RFQVWDAVRSLDYLAAHPLVDPKRLASTGQSGGGTLTMMLLAVDDRLAAAIECSGNTE
                     NFACANFNPPGSTDDAEQDFVGSGPEGFDRWDLLYPFAPKPLMVTVSDRDFFGTYSSQ
                     YIASGWEEFQKLKRVYEVLGRGDRLAWADTPLPHGLSYDSRLHVYNWLGRWLKGETRP
                     ITEEPPVEPEPDKVLAVSESGNVVRSFHGQTPFTLTRSRQLTRRSAPLDRLLGVERPA
                     GEVRAAVLRRVPSRGLEIEVLDIPSAPKVWAPAWLFLPNNPDPSQPAVISLEPAGRDV
                     RWHEGEMYQSLAQAGCAVCVPDLRGVGDLSPEFSRGAARYARSHSNEDNWAWASLILG
                     KPLLGQRVTDILAVVHALRSHPSLAGRRLTIAALGKMTVPALFAAALEPAVQSLYLAG
                     GLVSYRSIVETEQYTHPFCNFLPHVLEYTDLPEVAASLAPRRIVLAGAVDGSEQTMEP
                     AVVRRIYEKATNVEVLAAATWDATAFGKL"
     gene            complement(40617..42221)
                     /locus_tag="VFA54_17020"
     CDS             complement(40617..42221)
                     /locus_tag="VFA54_17020"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS52573.1"
                     /translation="MNKSGKRQNLLWIALGVAICVSALYWSLSSKGPEKPILETAPVE
                     VGDLTVEVPATGSVDAVNVVDVGSPVTGRIKALHVDFNDRVKAGQLLAEIDDSTYRAT
                     FLQAEANLAAARAAVNQERFNVESARSDYARATAEAQAAEATYRQAEADFNRMKRLLS
                     EGISTQADFDKSEATYRSARADALAAQAQSQVAAARVKSAEAALAQAQAVVEQRAAMA
                     AMAKRNLDYCRITSPVDGVVVARNVDVGQTVAASLQAPSLFNIAQDLSHMYVYTKLDS
                     SDVGKVRVGLPATFTVNAFPGEIFEGKLVSVRINANEIDPISKTTGLTSVFKKTLSAG
                     VTAGSVSASESVGGAAVSASQATNITTTTTTTFDPSGRIITTTSTPTITPATTAATST
                     NTNPSGLSIGLIGSNVPPDTTRNTVVTYDALIEFRNPGERIIPGMTAYVTIPVNSVTN
                     KMKVPSFALRFSPAIPEQEKQALLDANQLKPGDPMVWVVEGENKFRPVRVKPILTDYV
                     FTAVESDQLKPGMHVATRIVEPRSES"
     gene            42378..43754
                     /locus_tag="VFA54_17025"
     CDS             42378..43754
                     /locus_tag="VFA54_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52574.1"
                     /translation="MLLALILIAGLTDWVPARWISSDPKSLELIRGTPINCLLLERDQ
                     WSPAFAEEAARQNVVTLGVVRPGGDPLESALAALREKLQGVVLEGDFPAAEIARVKQK
                     LAAARAPVVEMLPRRSLDLHGQADIIATYQAVWPGIQAQDDEGKARAGPTGSPWINTN
                     TGFLIYARAAAEGPVWVGNLPPEKQVLTVENYLYAVCDAASLGARWVVALDPDFRGRV
                     LRREEKALRDWKRIALYLQYFEDHKEWRALRSHSRLTVLQDADSGALLSGSILDMMIT
                     KRTPVRLLPRSKLNEGALRGATMVLNLESDLLTPQQKETLAAFTHSGKTLLTSPPEFK
                     PPVLHGDQITVDEKEVGRIEDLWRGVNSSIAHANLGLRMYNVSSMLTNLLADSTGKRL
                     VLHLVNYSGYPVENITIFYPGSLKSAKILTPEKPAQEVEIFEQRGETTIELDRVGTCT
                     TLVLEQEG"
     gene            43756..44916
                     /locus_tag="VFA54_17030"
     CDS             43756..44916
                     /locus_tag="VFA54_17030"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZS52575.1"
                     /translation="MPEMTRRQLLTMLGAAAAWPVQKSWGAPEAPAAPVSIARCKSYD
                     ESLVATLSTMFDQLGGLQKLVKGKTVTVKLNLTGDPGLRLQGKPVGSTHYTHPTLIEA
                     FLHLADQAGAKRIRLVESCWATAGPLEEYMLDSGWRVRRFQSAAKNVEFENTNALGKG
                     KQYSRFKVPGQAYMFPAYDLNHSYADTDVFVSMAKLKNHATCGVTLSLKNCFGMTPAS
                     IYGEDAGEFEPNESPGSGRGAVCHYGKRGPSKCSPQELHPESPREGGYRIPRIVADLT
                     AARPIDLAIIDGIETMIAGEGPWVRGTAPAQPGLLLAGLNPVCTDAVATAVMGYDPRA
                     DRGSAPFEKCDNTLKLAEALGAGTTDLKRIEVLGVPIEQALFPFPKHLNMTG"
     gene            complement(44959..45852)
                     /locus_tag="VFA54_17035"
     CDS             complement(44959..45852)
                     /locus_tag="VFA54_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52576.1"
                     /translation="MSEGMNVPNSEVRPPSGEPAPVHPARGPLNLLAWVLVFTCIGGL
                     GVREAWRDRQQQRELAASRDQMTSALAQSQAEIQALRAKLDALDASREAPPAHPAKAA
                     RKPGGASAGRTRQARPLEDARWRQMQAQLSRQGEQIASTQKALSQTREDMENKLNSTR
                     DDLSGSIARNHEELVLLEKRGERNYDEFHITKSKQFQRVGPLSLSLRKTNAKRKSYDL
                     ALIVDDVELQKKGVNLYEPILINLSDRPQPLELVVNKITKNEVSGYLSEPKYKKSELA
                     AASSPAAGAQTPGLQPRRDSF"
     gene            complement(46058..47290)
                     /locus_tag="VFA54_17040"
     CDS             complement(46058..47290)
                     /locus_tag="VFA54_17040"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZS52577.1"
                     /translation="MTSRRSFLKTAAAGSLLTTAGARAARTNTYSNDIEARIARRDFR
                     GLSKAELPTPCMIIDQALFEQNLQKMANHCKTAGIHLRPHVKIHKSVDIARRQLALGA
                     IGLTTATIAESELMSGAGFKNVLWTKQPAGINNISRAVALSKRDPTFMFVVDDPLVAD
                     WVEEAAAAHNVRLKIVADVFAGLTRHGMQPGQPALELAQKIDSSKHLELRGLMGYSGG
                     ASHTHGWEARRKRSAGDLAGLRETAELCRKSGLPVQIISGGSTGTYNIDKENGLTELQ
                     AGSYIFMDTAYFAIGGKDDDSMYSDFAGSLTVLATVTSKRWPNQCTIDCGNKAMLRPT
                     DNVKGMPWIQVANQGAEYGILKWKDGDQDLKLGDRVELYCTTLDMSTNVYDRYYVAQG
                     ERIVDVWPIMGRAGAAQR"
     gene            47515..48858
                     /locus_tag="VFA54_17045"
     CDS             47515..48858
                     /locus_tag="VFA54_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide isomerase"
                     /protein_id="HZS52578.1"
                     /translation="MRVLAAGLLLTSLAAAASTPAAPTFSKDIAPILYKHCAGCHRPD
                     DIAPMSLLDYKSARPWAKAIREAVLTRKMPPWFADARYGKFSNDARLAPEQIEAIERW
                     VDSGAPEGDPKDLPATPLFTEGWKLGKPDIVVDIGEDYVVKPGNDSYEHFIVPTNLQQ
                     GVWIRAAEIRPGNRKVVHHVHVNLVQDEKQAGPTSIESMKSLNDFLIREGKLTRVRED
                     APVADNGCSADAANLPYMRGFQEGALASFLPGRAPDVFPEGSAKWVPANSKFEFVIHY
                     AKISRDAQTDRTSVGLYLAPKPPEQALRRMDLRNFFFRIPPGDPSHEVTRCYTFERDK
                     VLLSITPHMHYRGKDVTYDLTLPNGKHETLLLVPHYNFNWQLVYRFQEPVYAPKGSVL
                     KVTAHYDNSPNNPFNPDPTKAIRWGDKSEEEMMTSWIEYVDGSSLRQTAAYAPAK"
     regulatory      48966..49092
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            49135..50190
                     /gene="nadA"
                     /locus_tag="VFA54_17050"
     CDS             49135..50190
                     /gene="nadA"
                     /locus_tag="VFA54_17050"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZS52579.1"
                     /translation="MSSAITFDLWHERFSRFAEDLYPGRYSRDYVRTLVELGQQVEWL
                     ARQKRSTIVAHNYQYPELQEVAETVGDSLGLSQYVAKKQSPRVDFCGVLFMGETAKTI
                     LGDRSKVYMPDRPGCSLVASIDQRRLDHWLQANPDGVIISYINTDAVTKARSHYVCTS
                     RNAPQVLSRAAADNPGRRILFLPDKYLGAFAASQARVDRSLVDLYDGSCHVHSKISET
                     ALEEAIDLHPDAELLIHPECGCASSCLARTAQAGPAYGKSYYLSTEQMLWHARQSPAQ
                     EFIVATEMGMVYRLRKDVPEKRFYPVSYQSSCEYMKMNTLEKLLESLQCDRVEIEVDP
                     EIRRPARAAIERMLAIQ"
     gene            50206..51219
                     /gene="moaA"
                     /locus_tag="VFA54_17055"
     CDS             50206..51219
                     /gene="moaA"
                     /locus_tag="VFA54_17055"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HZS52580.1"
                     /translation="MLLDTYHRPVKDLRISVTDRCNFRCTYCMPLDEYEWIERREILS
                     YEEIARLARIFAGLGVEKIRLTGGEPLVRKHLEQLVEKLAGLPGVKDLCLTTNGALLA
                     EKAAALKAAGLKRVNVSIDTLDAEKFRKITQRGDLSRVLEGLFAAREHGLRPIKINAV
                     IERGVNDDEIVDLAAFSRKNGFAIRFIEFMDVGNTNNWRSERMVSKKEIIEKIRAHFP
                     LREAGRSDGSAPAVDYQFLDGSGDIGVIASVTEPFCSGCTRARLTADGKLVTCLFSST
                     GHDLKKLLRGGASDAEVRDCISAVWRGRNDRYSDERLEAMLSGQGYQPKAHRKIEMIS
                     LGG"
     gene            51247..51687
                     /locus_tag="VFA54_17060"
     CDS             51247..51687
                     /locus_tag="VFA54_17060"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HZS52581.1"
                     /translation="MSRLFTLQEAQALLPQVERWLRSAIENRKSAFQMDEQLNALLMR
                     INLQGGILPDVNRAAELKTGKEQSLDRLKKALSEIEEAGCLVKDLEIGLVDFPTLLDG
                     AEVYLCWKLGESQIGFWHHTHEGFAGRKSIDREFRERHKGSKSQ"
     gene            complement(51867..52691)
                     /locus_tag="VFA54_17065"
     CDS             complement(51867..52691)
                     /locus_tag="VFA54_17065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZS52582.1"
                     /translation="MANLRMCPHCRAFVDSSERICTECHEPLGRTYAQRVTAGAALAG
                     LVPPTHFTTIIILLINGALFAATVLLSMQRSGGGLWAVNSEVLYYFGAKESLSILTQG
                     QWWRLVTAGFLHGGLLHILMNSWALYDLGAQTEEAYGTARFLVFYLASSVAGFAASTF
                     WSRALSIGASAALFGLIGAMIAFAQRHRSTLLGMAMKSVYTRWAVYGLLFSLIPGIDI
                     AAHVGGLISGFVLGYVAGVPGPNRAIEWCWKAAAVVAVLIVGASFLLAWASFQPPT"
     gene            52979..53950
                     /locus_tag="VFA54_17070"
     CDS             52979..53950
                     /locus_tag="VFA54_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS52583.1"
                     /translation="MAAKSRGRKESTPALKRRAWLGRAGLAGIAGAAGLAYGAAPRLW
                     NEMARETLRPVEPPPERPRPFQWPSTGICAAWLGHSTVLLRIGGCTILTDPIFSERAG
                     VSLGPITLGIKRLVAPALSLEQLPKIDLILLSHAHMDHFDLPSLARLGSLQTTVITAP
                     NTSDLLRVKRYRQVRELRWGERFRVGPVECRAIEVKHWGARMRSDTYRGYNGYLIEAA
                     NRRVLFAGDTAHTSAFRALRDSRPIDLAIMPIGSYEPWIRNHCTPEQAWQMGNDAGAE
                     YFLPVHHQTFRLSREPLREPIERFFSVAANQSYRIRLEKIGQEFWLA"
     gene            complement(54012..55046)
                     /locus_tag="VFA54_17075"
     CDS             complement(54012..55046)
                     /locus_tag="VFA54_17075"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HZS52584.1"
                     /translation="MQSVQLVARERVELREMPEPREPGPGEVTVRVRAVGICGSDMHF
                     YREGSCAGSAATYPIVLGHEPAGEVAAVGPGVQGLRPGTRVAIEPAITCGECEFCRAG
                     RHNLCEKVVFMGGLQLPGFLREWASIPARNAVEIPSSMSYPVATVVEPLAVILHALEL
                     TRLEPGDTVAVMGAGPIGLLAVAVARIAGAGRIISADRIPCRLALAQELGADAAIDVA
                     RQPAAEAILDLTSGRGAHLIIDAAGKPDSLNPALHAVRHGGRVVLIGIPSEEMPRVDL
                     YAALNREATLLALKRSNRNDHTALRLIESGKIDAAKLITHRFPLSQADKAFQTAAAYA
                     EGVAKAVIEL"
     gene            55144..58674
                     /locus_tag="VFA54_17080"
     CDS             55144..58674
                     /locus_tag="VFA54_17080"
                     /inference="COORDINATES: protein motif:HMM:NF009589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZS52585.1"
                     /translation="MAQPPMRGAYSLDDKYTREEGIVFLNGIQALVRLPMEQVRRDRR
                     AGLRTGVFISGYEGSPLGGYDLALQRVERLLHEHNIYFVPGVNEEIAATSVMGSQIHQ
                     LFPRPKFDGVVGIWYGKGPGVDRSGDALRHANFAGTGKNCGALALAGDDHASKSSTIP
                     HQSDLSFFNFAFPILYPGNTQEILDLGLYGIALSRFSGAWTAMKMVTNVCDGGGTVEV
                     SPDRNPVVLPELEFGGQPYRKRMDGMLISPRSLQLEQEIHYRKLEAARAFVRANRLNR
                     IVASNAGDWLGIITAGKSYYDLCTALRDLGLDKENLARAGLRILKLGMTYPLEPAIIA
                     EFAAGLAEILIIEEKRSFIELQLRDMLYNRRERPAIYGKHDERGELLFPVHGEIDPDL
                     IAKVVGRKLLERSETQSALDGIAKRLSRIAGLEELPREPRGTPPLGTIEARPPSFCSG
                     CPHNRSTAVPDGMFAGGGIGCHGMAGRLQHIHRGFSYITQMGGEGAPWIGIAPFTETP
                     HLFQNVGDGTFFHSATLAVNACVAAGVNVTFKILYNNAVAMTGGQAAAGALPIPELTR
                     QLEAQGLKRIVVLTDDLEKYRDRTALAGSAEVRSRDDLDRVMEDLAAVPGATALIYDQ
                     QCAAEKRRLRSRGSLPKPERLLVIHEEVCEGCGDCVAKSNCLSLYPVETEYGQKTRIH
                     QPSCNFDYSCLLGDCPSFVSVVVEPGTGLKRRVLPLLPAMEIPEPAAKAAIGENETYE
                     ILIPGVGGTGVVTVNALLATAALLDGFHAATLDQTGLSQKGGAVVSSIVVGRRPVELA
                     NKSSYGSVHLLLGCDLLAAGAPDNLKRTDPRRTAAVINSAEIPTGEAVRKGTVHFGGP
                     LIDIIRRSTHPGSTVFVNATRLAEALFANHMPANIFLLGVAYQAGYIPLSAPAIERAI
                     ELNGVAAAQNLNAFRWGRRYYYDPEAVEEIAAPAETSAGKPVRSLDDLIERRCRDLAA
                     YQDALYAQRYRELVEQVRRREQEIRPLSTDLTEAAARCLYKVMAYKDEYEVARLLTKP
                     EFEQQTRAMFEAPRRIEYNLHPPLLRSIGLQRKLGFGPWFRPLLRALAALRILRGTPL
                     DVFGLSPLRRRERALITWYGQTLRAVLGGLHESNFDLAVQIARIPDAIRGYEQIKLRS
                     IAAAEAQAAALLAEFTAAPAATHSSSSVHL"
     gene            complement(59085..60965)
                     /locus_tag="VFA54_17085"
     CDS             complement(59085..60965)
                     /locus_tag="VFA54_17085"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZS52586.1"
                     /translation="MRVSIASALLFFLLLGSACRKPAASPAPDSADWLHVVLRGRTVH
                     NSHYLAASFLYQLQTLLGTAAGSPKVMSGRIVRREGKEIYIDLKQVPPNAAALLRQIA
                     GRQSPGKLSSEQMDAYMAEHYYRLTSPYPNLAALLREHPYRENPNWLAAPVDSLTFKR
                     RRVLHIAKDRIADALISYFDNNNRLLYPEGTVIAAESFDKEGRFVEAEVLRKRGDTFW
                     NFSVYDRNGALVLQSVAFDEEGRIAPEKEGFHVFQSCALCHRIDRLDLSGDAEAPVQA
                     PIREFFHKLPARVPQIHLGPEYYDHMAFTELTEANGKIKDGVFGVYGSLLLSELACRK
                     RLGELTADDRFRYERLRPYFPELLTPLERIDSFTNSIGLRLVRIPPPHEGMMIGSLSS
                     DPYHRRDELLHQIRFHHGFFMGMYDVTNAQFRRFRPNHHIAPYRGVNLDGDEQPVVNV
                     SYDDAQAFIDWLNSLPAERAAGRTYRLPTEEEWEYAAQGSDTGEARRFPWGDQWPPPE
                     GAGNFADEANGAYFNWEYLRGYHDPYLGTSPVGKFWPNPYFLYDMAGNVYQWTSSYYE
                     PYPGAAGGQPYSKSLRVIRGSSWADELPKVLRCAFRNPTPPAKKMEFLGFRVAADIPE
                     LR"
     gene            complement(61170..62129)
                     /locus_tag="VFA54_17090"
     CDS             complement(61170..62129)
                     /locus_tag="VFA54_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS52587.1"
                     /translation="MNRRTFLGGSVGIVLADAAAKGASMPIIDTHIHLFDPRRPQGVP
                     WPPKDNTILYQPALPERYRKIAVPLGVTGAIEVECSPWLEDNQWVLDIAAKDTIIVGT
                     IGNLEPGRPDFHQQLERFHRNPLFRGIRYGNLWGRNLGAELSKPEFISGLKALAEAGL
                     VLDTANPTPGLISDVVRLTDRVPNLRVIIDHLAQLEPPTDAAAGKAYQEHLRELGKRP
                     QVYVKVSGVLRHVNGRVPDDPNFYRPRLDEFWEIFGENRLFYGSDWPNSDQWGSYPQV
                     LNVVQRYFSAKGPAVAEKFFWKNSTVVYRWIHRDRTQPQSSTL"
     gene            62385..62879
                     /locus_tag="VFA54_17095"
     CDS             62385..62879
                     /locus_tag="VFA54_17095"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZS52588.1"
                     /translation="MTFWPRTALQLALGVAVLLQTLPAAQLADKKALTLEIAKQIAAA
                     AEKEAAGNKWTMVIAILDDGGNLLYLERMDDTQIGSIEVAIQKAHSALAFKRPTKVFE
                     DAVAGGRTAILKLPGAMPVEGGLPIVWEGKVIGAIGCSGGTSQQDGLAAKAGVDALTK
                     ILGR"
     gene            complement(62991..>63172)
                     /locus_tag="VFA54_17100"
     CDS             complement(62991..>63172)
                     /locus_tag="VFA54_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS52589.1"
                     /translation="HHLENWLQAMRDRKQPDANIDHGFSHAICCIMAAQSYWSGKKLY
                     WDPQKEEIIDQPVNV"
BASE COUNT        12322 a        19202 c        19432 g        12216 t
ORIGIN      
        1 tccagcggcg gactgtaagg gaccgggcaa tccggcgctg tgatgcggac aatcggcccg
       61 tccaggtaat cgaaggcctt ttcgctgatg cgcgccgcca gctctccagc gagacctccg
      121 gtcatcgtat cctcatgcaa caggatcact ttgccggttt tgcgaactgt ctcgagtacc
      181 gccgcctcat ccagcggcgc caaggtgcgc aggtcgacga tctcgaggtc gataccctcg
      241 ccggccagag tggcggctgc ttcgagggct acgtggacca tggcgccgta ggtgatcacg
      301 ctgatatcct ggcccgcccg ccgaatggcc gccttgccca acggaacaac gtagtcgccg
      361 gcaggcagat cctccttgac ctttcgatac agcgccttgt gctcgaagaa caaaactggg
      421 tcgttatcgc ggattgccgc cttgatcagc cccttggcat catatgccgt agccggggcc
      481 acaactttca ggccgggcgt gtgcacgaac ggcatttcgg ggttctgcga atggaacggc
      541 cctccatgga tgccgcctcc cgagggtccc cgaattacca tcgggacccc ggcgccccat
      601 cggtaccggc tcttggctgc aaaattcaca atctgatcgt aagcgcacga gatgaagtcg
      661 gcgaactgca tttccacgac cgggcgcagg ccggcgatcg cggcgcccgt gccagccgcc
      721 acaatggctg cttctgacaa gggcgcgtcg atcacccgcc gctcgccgaa acggtccagc
      781 aggcctgcgg tcactttgaa tgcgccgccg taggcgccga catcctcgcc tatcacaaac
      841 acggagtcgt cgcgctccat ctcctcccag ataccctggc ggatagcctc gacgtaggtg
      901 gtggggccgg cgctcattgc cgattccttt ggcttattcg taaaccccgt ccagcagggt
      961 ggagggatcg ggcatgggcg actgctcagc ccacgcgacg gcttcgtcaa cctccgccag
     1021 aatacgcgcg tgaagacgct taaaatcgac ttcggtggcc cagttgcgtt cgaccatata
     1081 cttctgaagc atcaaaatcg gatctttttt ggaccactcc tcaaatttcg ccttgggcac
     1141 atactcggcc gcgtcgtggg ccgagtgacc ggtcatgcgg aacgtcttgc actcgatcat
     1201 ataaggcccg cgccctccgc gtgcatgcgc gacggcccgg cgggcggcct gatgcacggc
     1261 gattacgtcg ttgccgtcca cgatctcggc gggaatgccg tacgccgaag cccgatcggc
     1321 aacattggga accgccattt gcttttccag gggcgtggag tatgcgaatt ggttgttatt
     1381 gcataaataa acaaccggga gtttctgaac ggcggcgaaa ttgacgccct cgtgccagtc
     1441 cccgcggctg gtggccccat cgccgaaata ggagaagact atgttcggct gcccgcggta
     1501 gcgcatcgcc agcgcgatgc cgccagctac cggcacactg gctcctatgc tgctgatgat
     1561 ggggagaata tgcagatcca gatctcccat atgcatgttg ccgtccctgc cccgcgtcgg
     1621 acccgtcgcc cggcccagat attgtgcaaa aatccgtttc gactccatgc cgcggaccag
     1681 aaatgctccc atgtcccggt gggtcgggca gatccaatcc cctttttcgg ccagaatggc
     1741 gctgcccaca ggaatggcct cctgcccgcg gcccgtgtaa cagccgccaa gaatcttccc
     1801 ctgccggtag agcttgagct cgattcgatt ttcaatctcc cgcatcagct ccaggtagta
     1861 gagcatgcgg ctgcacagat cctgcgttac ctcgggcgcg actggcgtag cggttgtcat
     1921 catggcgaac gtgtcgtaat gtagcgcaac agattgggct ttgcaagtgg atggccggta
     1981 ctgtgggccg cggtactgga gcagggaact ttttgctccg gctcctcgta tcaccttacg
     2041 tcaggcaggc aaacggatat ggccacagcc cccaacgccg ccgcgtattg ccgccgctgt
     2101 ggagtcatgt tccaagccga ggatcgctat tgccgcaatt gcggttacga ctctgccgct
     2161 cctctgggtg cctccgttcc cacaggaacg ccggcggagg cttccagccg gaggcgcagt
     2221 gcggcggcgc tgctctgttt cttcctgggg gttttcggcg cccatcgttt ctatgccgga
     2281 cgaatcggaa caggtctcct ctggctgttc acattcggcc tgctgacctt tggcatgctg
     2341 tttgatctga ttttgattct tgcgggcgaa tttcgggatg ccgaggggcg caaaatcctc
     2401 gtctggtaac gcccgcgaaa gttacagagt ggctgccctc cgcacggccg gcttgcggcg
     2461 caagaccagc agatagatca gcgcgccgcc ggccatggtc agcaagccca gccccgattc
     2521 tttggggcgc agcagcacca tatagatcag cacccaggcg ctgatggcta catagacgcc
     2581 gggaatcagc ggaaatgcga cgtttgccgc cggcaaggat ttccagccgg gacgccgtct
     2641 cagacgaaac actcccagca cggcaagcat tgtgaaaagc cagaggctaa agccgacgta
     2701 ttgtacgagg ctttcgaacg tgccggtgat gatgagtacg cagcagcagg cgccctgcgc
     2761 ccatatggcg acccacggcg tgttccagcg gggatgaatg cgctcggcgg cgcggaaaaa
     2821 agctccattt tccgccatgg cgtagtaaac gcggggtccg atgatcgtca tggcattgac
     2881 ggtggaaaga agggccagcg ccatcagcgc gctgaaggct ccggcgaccg ccggcccgaa
     2941 gagggaccgc gctgcgagcg atcccacgcc tactacgcct ttcatcgcat cgagcggagc
     3001 accgtagatg aatacgatat ttagcccggc gtagagcgcc gcgaccagcg ccgttccggc
     3061 cagcagagac cggggaagcg tccgctcggg gtcgcggagc tccccggcca cgtaagtggc
     3121 cgcgttccag ccgctgtagc cgaaaaacac ccacaccagg ctgataagaa attgcacccc
     3181 cagcgagtgc ggcgatgtac gctgcgccgc tgccatgaaa tgaccccagt cgccgtgtcc
     3241 gatgctcgcc cccaacacga tgaagcacac aatggtgatg atcttcagag tggtcagcag
     3301 attttgtatc cgtgcggccc gaagaagtcc aaagacgttg agcgtcgtga acgccaaaac
     3361 cacggcgcaa gccaccagtt gcgccggccc aagatgcact gttagaaagg ctgcctgaat
     3421 cgaacccgag ccgccggctt taagagccgg gaagaaataa gccaaatagc cggcaacagc
     3481 cagcgctccc gcggcgatcg gcgccgaaaa gcccgcaaag aatgaaaccc agccggtcag
     3541 aaagccccac ccgggtcccc aggcttcggt caggtagaca tactcgccgc ccgaacgggg
     3601 aaagttgatc ccgagctcgg agtaacagag cgcgccggcc agagccagca ccgcgccggc
     3661 aaaccagatc gccacaacca gccacgccga tccaagatcg ccagccagaa aaccggtggt
     3721 cgtgaagatc ccggtaccga tcatgttcga gacgacgatc gccgtggcgc tgaaaacccc
     3781 gagttcgcgt agcagcttcg gttggggcat gggctacaat ctataccaca agcatccctc
     3841 ggcgctcttc cgtgcggctc gatgcagctc tccagcggct tcgcaatcga tgtacaacca
     3901 aaagccggcg cggccggacg gcgcgatatc agtcgtcgct gctgaataaa tcagcgatgg
     3961 tttccagtcc atgctgctca tcgccgcccc gtcttccgcc tcccagttcc cggcgcagtt
     4021 ttgagagagt catggattgg actatcgcct tgccgggccc ccgcaatgtc gccagaaaca
     4081 ggccttcgcc gccgaagatc gcggtcttga tgccgccggc gagctgtatg tcatagtcga
     4141 cgccttcatc aaagccgacg atgcatccgg tgtcgacctg caagacctgc ccgggtccca
     4201 gattgaagtg cacgaagtcg ccgccgccgt gaatgaagac ggttcccggc ccggtaaatt
     4261 tttcgagaat gaaaccttcg ccgccaaaga agccggcccc cagccgcctt gtgaactgga
     4321 tgccgaaggc gaccgatgcc tcagcgcaga cgaagctgtc gcgctggacg agcacgcttt
     4381 gcccagccgc cagttcgatc gcctctagct ttcccggata atctccggca aaaccgactt
     4441 ccccttctgc cccgagcgta cggaaatagg tgagaaagag cgactcgccg gtaacggtac
     4501 gccgcagcac gcccatcagc ttctggccga tcgtctgccc gctcatacgc gtttgccatt
     4561 ccacgttggc cgttttgtag accattttgc cggcctctgc gtaaagctcc tgccctggct
     4621 tgagcttgat gcgcgccact tgcaagttgt caccttcgat ggaatattcg agcggcaata
     4681 cccggacctg gccggaggcg cctgcgctgc cgccagcggg ggacccgcag ttggcgcaaa
     4741 acgcggatcc ttcgggaagt ttgaatccac aagacgtgca gaaaggcata gcgcgttgat
     4801 tcgcaggaac gaaccaggat atcaggaatc aaaaacggag acgaatcggc aggggcagaa
     4861 tgaagcggag acgtaccgcc cggcgcaaac atgctttccg cgcctgcgcc gcgctttcgg
     4921 ctgctatggt acgcctacgc ctgagacaaa aggctgccta ccctggaaaa gatggtgcta
     4981 atggaggaac tgactggggg gaaagccgct cctatagcca cggtaataaa gccggccatc
     5041 agcgcatact ctaccaagtc ctggccgcga gtgtctgtcc acaggcgagc gatcacaagg
     5101 gctttcgtaa tctgtgccat gtccacttcc tttgccccca acataggaac agcgtccacg
     5161 caagggcgtc cagaggcgat cataagggac actctcccct cgtacaattc gccgaaagac
     5221 atattcttga tgtgtccata gtcgtatgac tccggcaaga caaaacgatt gtctctggcc
     5281 tggtgtacgg cgggaaaaca aaaaagcgcg ccgcggaggc tagggattct ggagagtcat
     5341 gcggacggaa tgcaggctgt cgtcagtgat ctcgatggtc tgttcggcgg cgcccactcc
     5401 ctctttgctt gcggtgatga cgtggcgccc gggcggcagc ttcaaggctt tgggggtttc
     5461 cccttcgacc ggcttgccgt caacagtgat ggtcgctccc ggcggcacgg tggaaagcat
     5521 aaccgtgccc aggatcgggt gcatcgtaat ctgcacgtcg atcggccgat tgcctaccgt
     5581 aatttcccgc ctttcggtct gaaaaccggc ttgcgacagc agcagcgtgt gcgagcctgc
     5641 cttcaccgga atggaacagg gcgttatgca agcggtcaga tcgtccccat cgacgacggc
     5701 tcgcgcgcct ggctcgctgg tggaaatgtt aaccacgccg ccgccggcgg ccgtcttgga
     5761 ggcattcgat cctgagcgtt ccgcaccgga tttttctatt gggatcggct tttccggggc
     5821 tgtcgaggca tcttgcggag cggctgcggc cggaggcgcg ctcgccagat tcgaggcggg
     5881 cgccggcggg ttgggcggcg tgggcgccgt gcctgccggt tccacgctca tactgggttt
     5941 ctcttgcgcc ggctgggcgc ggtgcatctg ccagaaaatt tcgcctccaa ctgcggccag
     6001 caaagcggct acggcaaaga gcagcacccc ccggccgcgg gacaccggct ccgacggggg
     6061 cggcggcaaa ccagccctgg gcgcaagggc atccctgact tccagtggag gcagtttttc
     6121 ggctcgctct gggcctgggc cgggctcgat cgcggaggga gccgcagcac tctggctccc
     6181 ggcaaggctt tgcggcggcg tttcttcagc ggctgctgcc gcctcattta ccgccgcttc
     6241 tggaaaatgg atcgcctcgc tcggcgccgc agccgggccg agcggcgccg ccggcaatgg
     6301 aggagatcct gccatctcca cgaccgtcgg gagatcttcg gcgcgcgatg ctggcttatc
     6361 gggctgcgtc atcaccgccg gatcactggc ctcggttgtc gcgaacaggg aaggcatctt
     6421 ccggagcgtc ggcgtgaggt ttgcggtttg agccacggcc agttgcagag cttcggcgaa
     6481 atcgcggcag ctcgcgaagc gctccgcggg ctccttagcg agagccttca tcactacggc
     6541 attcaccttg ctcccaatgc tcggattgat ttccttcggc gggaccgggc tctcgctaac
     6601 gatcttgaag atgaccgtgg tgatgctgtc gcccagaaac ggcttctggc ccgtgagcat
     6661 ttcgtacagc accacggcca gagaaaactc gtccgaacgc ccgtccacca ctgcgcctcg
     6721 gacctgctcg ggggacatgt agctcggcgt gcccatcacc gtccccgtat gcgtgaattt
     6781 cgaggaggcg atgcgcgcga ttccgaagtc ggtgattttc accgcgcctt cggtattcac
     6841 catgatgttg cctggcttga tgtcgcggtg aacaatgccc ttgctatgtg cgtagtccaa
     6901 agcctgcgcc gtctgagtgg caatggatac gagatatttg gcgtctccca cgagcccggc
     6961 atccagcatt ttctcgagag tctgcccctc gacgaactcc atggtgatgt aggcttgatc
     7021 gctttcctca gcgatgtcgt agatggtgac aatccctgga tgcgacagga tgccggccga
     7081 ctgcgcttcg cgaaagaggc ggctgcgaag ctgttcgcgt tcttctggat gagccagatc
     7141 accgaggcgg attgttttga tagcgaccag ccggccgatt gtcgggtcct gggctttgta
     7201 aaccacgccc atcgcgccgc ggcccaactc cgacaacaca cggtagcgac cgatctgttg
     7261 cagcacgccg gctctccgtt aacgttaaac actttagcac aggcaaaggc ggccacattt
     7321 gtttcggaac gatggcacag ccatctgctg gttgcggcgc cggacgttcg ccggcgtgct
     7381 tcaaacgcgc atcgccttcg gcggggcgct ggcgaaaagt ggctgtggat ttactggggc
     7441 ctgcccacgc tgatgtactc gaagccgcgc cgccgcatct cttccggccg gaacatattg
     7501 cggccgtcga taataagggg ccgggccatc gactcccgga tgcgggcgaa atccgcgcac
     7561 aaaaactctt gccactcggt gaaaatgacc agcgcgtccg cgccgctggc ggcctgttcg
     7621 gctgactggc agtaaacgat gcgatgagag gcgctcggaa gcgcggcgca agcttccttg
     7681 atcgctcgcg gatcgtaagc ggccacagta gcgccttccg ccaacagttt ctcgatgacg
     7741 gcaagcgccg gcgcaaagcg gacatcgtcg gtgtcaggct taaacgccaa gccccatgcg
     7801 ccgatccgct tgcctttgag cacccatacc tcatttttga ttttttggaa aaagtgttcc
     7861 atttggagct ggttgatgcg ctccacttcc cgcagcaacc ggaaatcgta gccgtttttt
     7921 tccgcaatgt ggcaaaaagc ctgaaggtct ttcggaaagc aaaatccgcc gaacccgatt
     7981 ccggcgcgga ggaaactcgg gccgatccgg ctgtcgagtc ccattccggc ggcgacctgc
     8041 tcgacatcgg cgcccacctt ctcgcaaagg gtggcgatag cgttgatgaa ggaaatcttg
     8101 gtcgccagaa aggagttcga ggcgtgcttg atgatctcag cgctgttgcg gttggtcacc
     8161 accaggggcg tgctgcggcc cttccactcg catccctcgt ggatggggca atcgaagctt
     8221 tgccgcagaa tcgggtcata gatttcgcgg agcaacgctt cggcccgctc gctttccact
     8281 ccaatcaccg tccgctgcgg atgcaggaag tcgtgaatgg cggcgccttc gctcaagaac
     8341 tcgggattgg aagctacatc gaagggcgcc gagccgccat agatctgcat ggtggcgtga
     8401 atcctcttgc ccgtgagcac aggaaccgtg ctcttttcca cgatgagctt gtaagagcgg
     8461 gagtgcgtcg cgatctgtct cgtcaccttt tccacgctcg acagatcggc ctcgccattg
     8521 ggcagaggcg gcgtgcctaa acagagaaaa atcacgtccg catcgttgat atgcggctca
     8581 aggtcgctga aaaaacggat ccggccgcgc tcgctgttgc gctggagaag cctatccatg
     8641 tgcggctcat aaaacggcgt gaccccgcgc gcgagagcct caatttttgc cgtgtcggtg
     8701 tcgtgacagc ctacttcatg gccgatttcg gcgaaacaga ggcccacgac caggcctacg
     8761 tgaccggtgc ccactaccgt gatcttcatg cgttcatccc tgcgcctgca atgcagccgt
     8821 aattcgtaag tatgaaaaaa aggcggcgat cccgcaaccc aattcgcgcg acgccagtca
     8881 ttgtggattc attccgcagc ggcggaattc cgcgcattaa gctcgaggct gattccgaag
     8941 tcccgaatct gactgggtgg ggagccggga aaatcggaag ccgccggcaa accggctttc
     9001 ttactcccgg acgcgggcgt cgtaaacgag gtttgcgcct cgagtgacat ggacgctggc
     9061 gatttcagcc tttagtgcgt cgatctggtc gtccggctgg aaatcctcca cctcggtcga
     9121 gaaaatcaag taatctccct ccggcagacc gccgaaccgg tactctccca atagattggt
     9181 tttttgcgag cggagtccgc cgaggcggta ccgattttga gggtccagcg gatagcagat
     9241 gatggagacg ccgcgggcgg gctgcccgct gctgtccgtt acacgcccgt ggacccgtcc
     9301 cgcgtcccgg cgaatacgaa tcactgcgga agctctccgg ttcggcggca atacgaagcc
     9361 gctcagggct tctttgccgt cgagggtaat atcgtctaca accgccgact cgggacaaat
     9421 cacgaaaaat cgccagccgc cgggcatcag accggggctg gagtagacgc cttggcggaa
     9481 ttccaggcgg atgggctgtg ttctcgcgcc gttttccacg cgagacagga aaacgagaat
     9541 ctgcggatcg ttttgctcca cgcctttctc atccaccacg cgcacgctca cctgcggcgc
     9601 cggagtgagg ctgagagtat agccctcctg gtcgccatga atttccagcg gctgataggc
     9661 gcacagcttg ccctgcggag catccagttc ggccaccagc gtatagcgcc cgggcacgag
     9721 accgccgaag gcgaactgtc cgaaagcgcc ggcggtggtt tcccgctttt cttcgtcccg
     9781 gaataacgtg atattggccc ggctgtgagg aaaccgcggt gcgccgccaa caatcccgga
     9841 aaagtgcagc agcgcgccgg caatgggctg gatctgaatg ttttccgtct cgcggccgat
     9901 ggttacgttc accacgcggg cctcggccat ctccagagtt ttgggatagt aggtgggcag
     9961 caagccggtt ccgtccggca attccctcgg cgcggtagcg acgtagtacc ggccaggctg
    10021 gaggcccggg atccggaagt agccgcggtc gtcggtgact gccgaaaaaa ggacatgccc
    10081 cgaaatggcg tgcggctcgg ccgtgatggc gttgactgta aagtcgggga taccttcgcc
    10141 gttttcatcg accacccatc ccgtgatggc gcccaggcga tgcaaacgaa tttcggccga
    10201 aaagccggct ccggctccca gcgcgatcgg ccggcccacc cgatcccagc ttttctggcc
    10261 ataacggccg gtggcgaagt tcgcgcggct cgccttcacc aggtaatagc cagggggcaa
    10321 ctgatcgaat acataacggc cctggcggtc tgtcaacacg gtccgaaccg gtgatccatc
    10381 cgcctgggtc aacaccacgg cgctgcccgc cagggcagcc ccggtatccg cattcgtcac
    10441 gattccagaa atcgtcccca gcgactcggc cccgagaata cttgacagca gcagcggcat
    10501 ccaatagacc agcaaggcca gacggcgccg agttcggacc cgcaattcgc ccgtcatgat
    10561 cctacaatct gccggacgac catccgcccg cggtcaagag cttcctgata ttcctcgcgt
    10621 ttttcctgcg cggtgaagcc ggaccccagc cgcaaatagc gctcgagccc caatcccaca
    10681 acataggttc cggcgaacgc gatggcccct ttcggtatca ggccgcagcc aaaaggaacc
    10741 ttcgatacca gctcgcgagc gatcgcgcgc caaccgaaag cggaggtaat gatcgaagcg
    10801 atctgcccgc gctggtctat gaagccgacc ggcgcgtcgc tcgccgcagc aatcagaaat
    10861 gccatccgga tctggttcat cgtcagcaca gcgatgtcgg atgcgaattc gccgacagcc
    10921 cagggcagtt cgatgaagct cggcacgatg ttcggcaggg cgctggcgag ggcgaagagc
    10981 gcgttttcgc gggagatttt gcggatcagc cgttctatta caggacctcg aaacggcaga
    11041 aaatgccgcg ccaggggaat ccacagatct tcgcgctcct ccaggcagcg ttccacggcg
    11101 gcggtcggat gagccgcgtc aaagccgtaa aatgagcggc ctggatgctc cagatgaggt
    11161 tcggccaggc cgagatcgca ctgctcaaag tcagccgccg tctggatgcg cagcaggtga
    11221 gccgacgcct gccggagctt ctcctctgag atgccggtgg gcaataaaaa ttgcaccatc
    11281 cggtggtagc ccggctcatc ctcggcaagc aaaccgatgg tgaacggctg atcggccatc
    11341 tgctgcactt cacgggggtt cagattttgt aaagcgcccc ggatttgtcg aaaaatgtga
    11401 gtggacataa aagctctccc tgcggcgctg ttcaagccgg cttttccgcg cccttggccc
    11461 gaaaaacgac ccgcaagctc cgtcgccagt cggcgctcag accgattcgt tccacttcga
    11521 cctgttggga aaagtattgc agaatcgttt ccgtcgtggg gtggaaatgg aaaacgggcg
    11581 agaccagcat gaggcgcgga ggagtcggct ggatcgccaa accgggaaaa tagcctcgct
    11641 tctgaaattc gccacggtcc agatgccatt tcacccgcat ccaatagtca aggccttgca
    11701 gcggcaaatg gacgtcttcg gaagccttaa tctctactac cacgaggcgc ccgctgcgct
    11761 ccgagccgag caggtcgatt actccccgct ccgatccggc cacggcggga acttgggtgt
    11821 aaagaggctc cggcagcaaa gagccatcaa tcgcagccgg gtccgcccgc accatcgact
    11881 cgagccattt ctccggcgcc atgtggtaga acgcgctttc ccggggggcg gcatccgctc
    11941 tgcgctcttc cgcgatgcgg cgcgccagcg cctctacctc gtagaaattc ccgccggtaa
    12001 ccggcctctc gtcgcccact ccgtaggtca tctggcgctc catcgctcta gcaaattcga
    12061 ggccgcgcag ccgaatcgag agcacgccgt cgggccgagc caccgcctct gcgccgaact
    12121 cgtcgatgag ccgccggatc cattgggcaa ccgggcctgc cggctgggga accggagagg
    12181 caggatccag agaggtcgct aaattgccgt aatttttctc gtcgagaggg gatgtaaagc
    12241 cctgggaatc gaacgcaaac agttcgcaag aagcgcgctc gcgatccaga aacgccagcc
    12301 ggctgctggt ggcggcggtc cggccctggg ggagaaaaat tttcagtcct tccactgtgc
    12361 ggcggcgctc tctgtgccgc aagtaatgca gccagatcaa accgaaggaa agaatctgat
    12421 cgcaaacggc ggcgcgcgtg cttccggccc ccgcctcggg atcggcggca atggcggcca
    12481 gagcggacgt tcccttggtg agaaacgccc ggacgtaggc cggcgagagg gagtgctcca
    12541 gatcgggggc cgcgctgatc tgggcaatcg tccacccgcc gaactgccgc gccagcattc
    12601 tccggaaccg ttctcgaaat tccaaacggc cggcccgccg ttctatctcc gccccgaccc
    12661 gggccaggtc gagaaccagc atttctcctt ccccttgcgc cagccggcgc gtcaccagtt
    12721 ggatgcgtcc gttttcctcg cgcccgacgc gtagaatccg ccgcaccagg ctggtttccg
    12781 cactccagac atgcagcagg accgcgctgc ctctgatttg cagatcaaat cgccccggtt
    12841 ctaaagggat ggtcttttcc cctggttcga atatggcggg agagcggcac gcgcgcacga
    12901 actcctcaat gcgagcgtgc agcgcctcag ggggatcggc ccgagccgcc ttccggcgta
    12961 gagacatgga tcgactacga tcttaaccga tccaggatgg aaaccaacaa tcgaaggtgc
    13021 gggccctgct cccgttaccc cagcagggcc ttatgccact tcagtgaacc taccgacaga
    13081 ggaggcaagc cgaccaaaac taaaataacc tcttccgccg cagcgacgag gctgcggccc
    13141 cctagcactg tcgtgctgcc ggaattttcg cggcaaacaa gctgccagcc gataacttct
    13201 tgtagatcaa agcctcccgt ccgaactgga gctgcaattc tacaatattc cgtagacctg
    13261 actccggttt tccaaaattc gggaacatct gggattcgga aaaacgcact ggatagctgc
    13321 ggagcagatg cgtcggatcg aaaaatcaca ctgtgtcact cggagtttca cgccagactt
    13381 ttcgcacgcg ctccagaccg ttctcgaagc catctttgag ccgccgggga gcgagcgcca
    13441 cgattgcctt ggcgcactgc aataccgtcg gagccagacg cgactcagct accgattcga
    13501 tcggccgctt tcctggtgca aaggccacga tggctaggac gagcgccgcg gcaatcagca
    13561 ttccgcgcag caggccgaag ccggcgccga ggaggcgatc cacccagcgt acgccggttg
    13621 cctttaccag gctggccaga attttggaaa tcaaaaaacc cgcgaaaaga aacgcgcaaa
    13681 agatcaggac aaagccggcg aagcttgcca cttccatgtt cttcgcatag ggttctatat
    13741 gcctggcggg aagagggtac caccacagag cgcccagaat gcccgtgacc acggccacca
    13801 gcccaacgag ctcgcgagtc atgcctttga tgatgctggt gacaaaggaa atcacaatga
    13861 cggcggcaat taggacgtcc agccagttca cagcgctttc cctgtcaggg cccggtacgc
    13921 ttccaggtat ttttcgctgg ttttgctgac tacatcatcc ggcagcggag gagccggcgg
    13981 ctgcttattc cagtggatcg actccaggta atcccgcacg tactgcttgt cgtaggagaa
    14041 ttgggccccc ccgggcttat agctttcgag cggccagaag cgcgaggaat ccggggtgag
    14101 cacttcatcg cccaggatga ggcgtccgcc cgcgtgcccg aattcgaatt ttgtatcagc
    14161 aatgatgatg ccgcgcgaaa gggcatagtc tgcggctttg gaatagatct gaagcgtcaa
    14221 atcccgcagt ttagccgcca gttcggcgcc gacacgcttt tccacttcgc tgaaggaaat
    14281 attctcgtcg tggcccgtaa gagccttggt cgacggcgtg aaaatgggct cgggcaggcg
    14341 gtcgctctct ttgaggccgg ccggcagggg aatgccgcac acggtcccgt gtttcagata
    14401 ctctttccaa cccgagccgg acaaataacc gcgcgccacg cactcgatcg gcgccatatc
    14461 ggcttccacc accagcatgg accgacgatc gagttgctcg cggtagcgcg gcaacggctc
    14521 ggggtattgg gaaacatcgg ccgttacgaa atgagtgggc acaatgtcgc gcaggaagtc
    14581 aaaccagaaa atcgacaact gcgtaaggac ttcgcccttc cgcgggatgc cggtgggcag
    14641 aatgtaatcg aacgccgaga tgcggtcggt ggcgacgatg agcagccggc cgttcgcgcg
    14701 gtaaacgtcg cgcactttgc cccggcccac aagctggcaa cccggcagat tggtttgaag
    14761 gagaacttct cgtataggaa gtggcatgct gaaagagttc acaatcaaaa gaagatcata
    14821 gcccaggcgc ggctgttatg aaaaccatta ttgagccatt caagatcaag acggtggaac
    14881 cgatccgccg caccagcccg gccgaaaggc agcgctacct ggaacaggct ggctacaacc
    14941 tgttccgcat acccgcccgc aacatcctga tcgacctgct gaccgattcg ggcactagcg
    15001 ccatgagcac cgggcagtgg gccgccatca tgcgcggcga tgagagctat gcgggcagtg
    15061 agagctttta ccggatgcaa aaggtggtgc aggaaatttt cggcttccgc cacgtgatcc
    15121 ctacgcacca gggccgtgcc gccgaacgca tcttgttttc cgtaatgtgc aagccgggcg
    15181 acgtggttcc ttccaatacc catttcgaca ccacccgcgc gaatatcgag ttcgtcggag
    15241 ccaaggccgt ggatctgccg atacccgaag cggccgatac ccaggcccgg ttgcccttca
    15301 aggggaatat ggacgtcgag ggcctgcgcc gcctcatagc cgacgtgacg ccgcggcgca
    15361 ttccgctgat tttgatgacc gtaaccaaca actcggccgg cgggcagccg gtttcgatgg
    15421 agaacctgcg ggcggccagc aacctggcgc gggagttcca gattccgttt tatatcgacg
    15481 cctgccggtt tgccgaaaac gcctacttca tcaaactccg ggagccgggc tataaggacc
    15541 gcacgccgaa acagatcgct caggaaatgt tttccctggc tgacggctgc accttttccg
    15601 ccaagaagga cgcgtttgcc aatatcggcg gcttcctggc gacgaacagc gattccctcg
    15661 ccgaacagga agagaacttg ctgattttga ccgaaggctt tcctacgtac ggagggctgg
    15721 ccgggcgcga cctggatgct attgccgtcg gcctcgagga ggttctggaa gaagactacc
    15781 tgaactaccg catcgcttcc acgggttact tgggccgcca catcgccgat cacggcgtgc
    15841 ctatcgtgga gccgcccggc gcacatgccg tctacatcga cgccggccgc atgctgcccc
    15901 acattccgcg ttcccaattt cccgctcagg ctttggccgt ggaactttat cggcacgccg
    15961 gcatccggtc ggtcgagatc ggctcggtca tgtttgggga gaatgcccgg caggaactgg
    16021 tgcgtctggc gattccgcgc cgcgtttaca cgcagagcca catagattac gtggtagagg
    16081 ccattctcga ggtttacgag aaccgcgaga gcattcgggg tttcgaaatt acgcaggaag
    16141 cccgtttcct gcgtcacttc accgcgcaat ttcggcccgt cgccccgtag aggcgggcgc
    16201 tcaagcgcgt tcggcgctgt gcggccagca ttgcctgaaa gccggaatca ccgcgcggcg
    16261 ggggccagcc ggaatctcaa gaatacctga atcccaaggg gatggttgcc gtaaacgggc
    16321 ttgagaacat ccggcgtgcc gaaataagtg aattgcccgc ccaggcctgt actgagccag
    16381 cctgcgtagc gaggcagctc gtattcgtat cccgccgtat aggcctgcac gcgcgcatac
    16441 cggcgttcat ccaccagcag gacgaaaagt tgctcttcat agatcaacgt ggaatctttg
    16501 tccacattct ccacgcgccc ccagatccaa tgcttgttct taaagaatag agtggattcg
    16561 gcgaggtagg agttatagat ttgcccggga atccgggtgg gcgccgtcac gatgtgattc
    16621 ctcgggggag gcagaaacgg cggctgaagg aatggtgcgg tgccgctctc ctggccggcc
    16681 ggtgcgactg cgtcggccgg ctgccgagaa gccgcgaaaa actgggtaaa gttcggctgc
    16741 tgcgtatagg tcaaatcgtt attgcgcccc cacagcagag tggtcgccca gtgcccggtc
    16801 gccagcggcc gcacgtacat gaccgacgcc gttgtgcgga gggttgggcg cagcggatga
    16861 gtcgcttcgc gctggttgat ccggccggct gaaaactgtg ccgtccagcg cgatgacggg
    16921 gccactgtga ggcgtgcggc gaaggaatcg atcgcgccgc cctcgatccc ccagcgatgc
    16981 tcatcgggct cgcggccgtg aaaacccgaa gcctcgatcg tcactgggcc gtaggaaaga
    17041 ccggcggtcg ctacgttgtt cgaaatgtgc gtggagtcct gatcgtgatg cccgagcgtc
    17101 gcgatcggat cctcggaagc cgagagccgg tgcggatagg ccgtcggacc gagcgcaggc
    17161 tcgccgcggg ggccgccgta aaaatgcaaa actgttcgct cgccgagttg gatctggtaa
    17221 gaagcggcca gctccatgaa caaatcatgc gggtgctggc cgctgatgat ggggatgccg
    17281 ccgttggcgg tttcgccggt ttgaaagatg agggggtagc gttcgttggt gatcgtagcc
    17341 ggctccaggc tcaacatcgc gcggaaagta agcgtgccgg ggccgagacg ccgcgagagc
    17401 atgggcatga accagttggt ggagaaaaac ttgtcgcggc ctcgcggtcc ggtttgctgg
    17461 gattccacgg caaataattc gccatgaacc atcagattcc agtctccaag actttggtgc
    17521 agcatatcca taggggcggc cttgggcatc tggctggtgc cggaggcgta atttgcggcc
    17581 gccgttccca tgctggcatc ccccctgccg ctcatgttgg cgagggtgac ctcggagaga
    17641 ggaatgccgc tgaaattgcc gcgcccggat tgccagccat ccacgcaatc cccgcagacg
    17701 cctttcgttt gcgcctgccc cggcggcttc actcccgcga tcttgtaata ctcaggcgcc
    17761 gttccggaag ccagatcctg gccgcacggg caatcggctt cgtgagcgca ttggtcgcag
    17821 ccgccgcgaa tgcagcaaaa gtatcgcttc tccgctacca aggtttgttt ggcccgcagc
    17881 agggcgtcgg tggcttcttt cagctcatcg ggaggctcgc ccggcgtagg gcacgcctgc
    17941 ttctcggagg gcagaagctt caccgacttt ttattcacgc cttggattgc gccgcgcccg
    18001 gcctgccacc ctccgtagca ttcgccgcag gcgccaaggc ccttggcgac attcgccgcg
    18061 cagttgcagg agccgtttac acgcgcacaa aggctgcaag aaggcttcac gcagcagttg
    18121 tagcgtccct gtgcggccag tttcttcttt gccgccgcca tcttgcgctc ggaaagcttc
    18181 aggtcggcct tcgcttttgt gggttttggg ttcctgacgc cgccctcgtg ccccagaaga
    18241 gccgggctgg ccgccagcaa cagcgcccac agagaggcga tgctgcgccg ggcagtcact
    18301 gcgcagctcc gggctccgcc gcatctctga cctccagaat gaaatcggcg gttatgatcc
    18361 ggccttttct tttaaattgc gaccatccgc ggtagatgcc cgcctttggg aaccggacca
    18421 gaaacggcac ggttccgtcc cggcctgcac gaggctcgcg ctcgtccgga tgggaatgaa
    18481 cgaaagtcac ggaatcctga tgcacaagca tcagatggcc catggcccca aggtacggct
    18541 cgaggtcggc gacggcttcg ccggtggccg cgtccttgag ggcaaaaaaa atgtcctctg
    18601 ttttcttagc cggcaaatac gtttcgggcg ctttgagttc cacggtgacg ccgtcgcatg
    18661 tcttaacgcg cgagcgctcc ccggccgaag ctttggtgat atcgaacttg cgatctgatt
    18721 ccccgcccac gcgaattttc gccatcatca cctgctcgcc ggcgccggca ggagcggtgt
    18781 cggcgaagat atgatattca ccggctgccg gaaacgtgta ctgaagctga aagctcccat
    18841 cgccctgacg ctcgggatgc tcgtgcgcga agtgcgccag gtcgctccgc aggatcagaa
    18901 gatgcatgag cttctcgtgc atcacttcga atgacgagaa gatttccctg ggtttctcgc
    18961 gagcgcgaac aaccaattcc agctcgaccg gctggccggc cttggggttt ttcgggttgc
    19021 tcttcaattc cagttgaaac gggcgcgggc ccggcttccg cttccgcgcg tcatcggcgt
    19081 ccagaacctg caacggaaat gtgacctgga acgcctcatc ggcgggcggc tgaatcgcaa
    19141 gttgcaaccg gtattcgccg ccatggctga acgtcgggtg gacgccgtat tcgcccggaa
    19201 ttccttccgt gtgcgcgatc tcctcgtact tcggcatgct gggcatggcc ggcatgtcca
    19261 ctgtcgcgtg aatgcgcgcg cgcaccacgg gcgcggcccc tagcaaaggg tccacccggc
    19321 tcctatccac cacccggaac tcgatttgca tctcttcctg cgcgtaaagt ccgtcgggag
    19381 gcagccgcag catgacgtca tatttgccgg ccgtttgatg cggcggttcc tgagcggcga
    19441 aaccaataac ggacaggaga caaacgtaga gaaagagaaa gcgcatcggc gtttcagtcg
    19501 cgtatcattt cacggatatg gtagctgtgt tgcccgcctg gccgccaaca ctgatgaaca
    19561 tgacgacctg atctcccggt gttacgtcct gggggatctg gatattcgcc tgggcaagcc
    19621 cgacgaaagc cggcgtgagt cccaaaaaca ggacattagc cggctttcct cctatgttgg
    19681 cggcccacgg aagcactgcc ggagaaacat ggtcgagcgg cgccgcctcc cccgtgggta
    19741 ccggaggatt caccgctccc tggccggtca aatagacgac gatcacgttt ccgcgcgctt
    19801 ccggattagc cgggctgttg aggctgccat cctggttttg cacaaccgcc cggttgctcg
    19861 gcaggtattg gaagatgccc accgacgacg cggcgacgtt gaaggtaaga ggattgctcg
    19921 atcccgccgc attggtcacc acggccgtgg ccgttccggg agctgtctcg aaaggcagtt
    19981 gggcgttgat ctgcccggga gaaacgaaga acagcggcgc aggcgcgccg ttgatggtga
    20041 cagacgttcc agccaattgc gccggcaagg gagtctgcgt ggccgcgagc gtcgtcggcg
    20101 ccagattcag cccaaatatc gaaataatcg aaccgggagc gacagggacc ggagccggcg
    20161 cgaagctcgc gccattcacc acgccgcccg cgctgatggt gggctgggaa ctgaacgaga
    20221 ccttgcgaat cagatagttc tgccagtcta cgatgtaaag attgccgccg gcatcgatgg
    20281 cgatcccgcc cggaaaattc aggctgctgg ccgtggccag cactccgtcg ggtccgcgcc
    20341 ctggctgccc gtcgccggcg acggtggtga tcgtgccgcc gggagaaacg cggcggatcc
    20401 ggttgttgcc cgtgtcggcg atataaatgt ttcccttgga gtccaccttg acgtcgcaag
    20461 gcgagctcag cagggcattg gtggcggcta tgttgtcgcc gttgtaaccg gcttggccgg
    20521 ttccggcgat cgtggagatg gtgccgtcgg gagtgacctt tcggatgcga tgattgccct
    20581 gatcagcgat atacaaattc cccgccgaat cgagggcgat tcccgccggc ttgtagagca
    20641 aggccttggt agcggcaatg ccgtctccgt tgtagccgaa agccttgggt ccatcgggcg
    20701 tactttggat cccgccggcg acgacaacga gcttcccatc cggccccact ttgcccaccc
    20761 gatggctgtg catttcggca aagtagagaa cgccgctcgg atccaccgcg accgcgctcg
    20821 gccaatccat cggtgagttg ggcgcgggaa cgccaggcgc aaaggggttg tgcgttccgt
    20881 tgcccacgat cgtggtaata gtcccgccgg ctacctgccg gatgcggtta ttctgctggt
    20941 cggcgatgat gaggacgccg ttcggtccca ctgtcacatc tgccggattg aagagctttg
    21001 cggcagcggc cgggccgccg tcgccgacgg agctggtggg ctggcagaag ttgtcgatgg
    21061 aaggagcgtc gccgctgcct gcgacggtgt tgatcgtgcc gtccggcgaa atccggcgga
    21121 tccgctggtt gttggagtcg gcgaagtaga cgttgcccga ggaatcgact gaaatatgac
    21181 tcatttgctc caagggattt acccctggac acgaagggct gttcagattt gccagggcga
    21241 gcatagcgtt cacagcaggg ccgccgtcac cgctgtagcc acgcgcaggc gttccgctca
    21301 ccggtgtggt acccgccagg gtggtgatga ttccggtttg ggattgggct gggaggggac
    21361 ccaacgtcgc gaggaacaga gcgagaatta gacaagatcc aatcgagcgc aaagtatcac
    21421 cttggttccc aaaacgatct tccggggaat gtatgctgcc actctgagga cgcggttgga
    21481 acagtatacc ctttccctgt tccagcggtc aaattcgacc ggcgttcggc tttggattca
    21541 acttgttccg cagatctaaa attaaaatta taaatggcgc cggcgctgca cgcgctcagg
    21601 gggcgctcat gagcggaaag aagatcgtac ttgttgcatc ctcgaccgag gccaacgaat
    21661 actcccaaga ctgctggatt caaatgttgt tcgccactct tcctgccccg gtggcgaagc
    21721 attttgtcaa cctcgacgtt attttgcgga acgagacttg gccggacggc caggcaaagt
    21781 acgtgcccaa cggcctgcgg gtggtggagt ccatcctgct gcgcgagttc cctccggaag
    21841 atatcgcggt gtgctacccg gaccagatgg agcggttcat cgggagcgat acaagggcgg
    21901 ttggactgca cgcgcacaac ccactgggca tcacgttcgc aacggatgtg tacgctcact
    21961 tggcgggtcc tcgcacggaa ccgctgaacg ccgcggaatt taagcgggcg attctccacc
    22021 cggttttgcg gcagcataaa tcccatctaa agatcctcgt gggaggcccg ggcgcctggc
    22081 agatcgaaca aaccgggatg cgccaggaat ggggcatcga ttgtctggtg gacggcgaag
    22141 ctgagcactt ggtcctggag ttgttccgtg ccgccgtccg gggtgagccc ttgccggccg
    22201 tggtcaaagg cgcgagcccc gaaagtccag atcaaattcc tgtgacccgc caccgctcta
    22261 cgctgggcgt cgtcgagatc acgcgggggt ggcgggcgag gctgccaatt ttgcggcgcc
    22321 gctgtgcggc gcggccagag catcccgctc gataacattc ttcagaacgt gcgcgccaac
    22381 gtggccgacg gcgccgatac catcatgctc acaacagagg atgtgttcct atatgagcag
    22441 ggaccgcggt ggcagccgaa ccgagaagct ttgctgcggc tttttcgggc tgtagcggcg
    22501 gagccgggcg tgcaatatat tcatctcacc cacgcgacca tggcgcccgt ggtgcaggat
    22561 ccgggcatga tcgaagcgat cacgccgtac acagttggaa tgagccgcaa acgccaccct
    22621 gcctccacac atccggagaa ccgctacgcc agcgtcatca tcgggctgga gaccggttcc
    22681 gtgcggctgt tcaagaagta catgccgggc aaggcctatc cttaccggcc agagcagtgg
    22741 ccagacgtga tgttgaaggg gatggaaatt ttaaaccgcc acaactggtt tccgttctgc
    22801 acctggattc tggggcttcc gggagagacg gacgcggata cgcgggagtc cctggatttg
    22861 ttgcatgccc tcaagggcgc caagtggtgc gtggtaccta cgctgttcac gccgctcgaa
    22921 gatacacggc tggccagccg cgaggccgcc aagctgccac agctcacgga tctgcaatgg
    22981 gagtttttct ttacctgctg gcgctataac ctcgattttt accggggcgc gaagaaaaaa
    23041 atcctcttcg ggctgggtat tccgttgtat tactacttgc tcggccgcaa gctttttggg
    23101 gagcgcgtga agtacccgct gtggcggttg gcccatgtac cggagcggat catgcgaggg
    23161 cggctatatc tggatttgga ccgccctgcg acccgctttt cggtccccgt ggaggctcct
    23221 gttccgctca cggccgccac gccgcccgag cgggttggat tgatgagttt gcaatagcgc
    23281 ggctgctggg cagcgtggcg gcgccgggga gcgcgtcgga aatcctggtt tttgggcgta
    23341 gagccgctcg cggtgctgct ctatcgcctc ctggccattt gaaatcaccg ggaaaccttt
    23401 cactcgtgct ggcgatttca cgcacaagat ttcgtgcgtc tcggcgatga ctacgggggg
    23461 caaacggggc cctgggtgct ccttgtacct cggcgtacag caagtggagg gcgcccgcac
    23521 gcggatgaaa ccgactggcc gcgcagggcc gactcactaa agggggccga ccggctacgg
    23581 gccatccatg cacttagctt tcatttacaa cggggttttt atcgccatca tcgcccacgg
    23641 gctgattggg ggatcgttga tctgggacaa gattctccta cgccggcctg agacccgcaa
    23701 tttgctttca tacgttttct ggctgggagc gctgagcatc ctggggctct gcctgattcc
    23761 cttcggcttt accatgcccc ccattccgat tgccctggtc tcgtttggcg ctggtttgct
    23821 acacatggcc tcgatcttct tctactatga ggcgctcaag cgcggcgaag cctcgcaaac
    23881 actggccatt atgggaggct tttcgccgct tgcaaccgcg ctgatcgggg cgcccatcct
    23941 ggctcaaccg ctaggcaatc agcttctcgg ctttgctctg ctcacggccg gcgggttcgt
    24001 catgttcttt tccgagcgca tcgacctgaa aagcattgta ccgcccattc tgctcgcttc
    24061 gggtctgttt ggcttggtta acgtgctgat aaaagtggtc tatgaccaca cgaattttgt
    24121 atccggctat gtcggcttta ccctcggcac tgcggccggg gcattctttt tgctcgtccg
    24181 gcgttcgtgg cgaaagcaga tcttcgagca gtcggaagag gcttcgccgc gcagccgatt
    24241 ttggtaccta gtgaatcgct ttctgtcagg gataggttct tttctggtct attacgccgt
    24301 tagtctggct cacccggcag tggtggacgc cattgccggc gtccgctacg cgatcatctt
    24361 tatcggcgca ttcctcatta cacgcctgaa gccttcctgg ctcgcagagg atttctcggg
    24421 tcgagcgctg gccgggaaaa tcgcgggaac cgcgttagta acagcgggtt tggtgctctt
    24481 gggcttgcag ggaggcgaaa ccaatgcagt ttacctccgc cgcccggatg tcgtggcgtg
    24541 gttccaatct cgttgggcgt gaggatctcg ttgtcgcggc ctcgatgttt atctactgct
    24601 ggggaatggg gttacaaccg cccttgcctt tttctgttgt ctcttccgcg gaatcgccca
    24661 gagcggtgag ggcactgggc gctcaaggct gtcgattccc agccggatga ttttctcgat
    24721 ttcgctgtac ggcaactgcc actcgagcga cagatcaaaa atcgtctttc cggcgcggaa
    24781 cccgctgaaa accgtgcgct ggaaagaggg cgggagccgt tctaaggttc gcgtcatcgg
    24841 cgcctccact tggtccgtaa acgcacccac agcaacccgg atgccaaagg acgtctttac
    24901 tccgatgccg tcgcgtttcg cgctctccgg ctccagcatg ttgctccaga tagcgagtgc
    24961 cggcggggaa tctggtgggg cccggcaacc tcccggtaaa aaagaaccaa gtaagggttt
    25021 gggcggctat ggaagttttg ccgccccgcg ctgttcgggc gtagccgtag cccagacctg
    25081 cctgcatagt cgggcggagt tggtctccgt tttgctggca agtgcgcctg ctccgcgccg
    25141 catgccggcc aggggcaatg ccgaaaccga gcacggcaga gagtggggag taagaccgtg
    25201 gatccccgaa agcgcaaaac tagtcttctt cacccggaac tcggctggcg ggccaaaagg
    25261 ctctcgacgg cgttgggctg ggggagacgg cgacagccgt tgcaattcgc ccgccccggg
    25321 cgtatgcttg gcgtgggcca acctcagaac agccaataca accgacagga ggccgttatg
    25381 gcaaaagtga tacggtgccg cgatgtggga gtcgactgtg actttgaagc gcgcggcgaa
    25441 acggagcagg aaatactgag aaagtgcgcc gagcacgcta aatccgcgca taacatggat
    25501 tcgattcctc ccgaactgga ggccaaggtt cgaggcgcga ttcgcgacga ggaagcagct
    25561 taggccagga gccgggatcc gttctttcgg gcggcaagtc ccggcagaga atgtgtgcac
    25621 gggcgccctc ccgtgcacac cctcagacgg caatctccct gaacttgtac ttcgtctggt
    25681 ataccgcttc attcacacga aggctcagac ccggagtttt agggagcgta taaaagccgt
    25741 tgcggaactc gtagccgtcc gcaatgatgg catcacagcg cgaacggtcg tcttcggctg
    25801 tgatggcggc agcaacggct ttggcaaagt gcagattcat cagcccgcct atttgagatc
    25861 cccagttgtg agggacgcag aatccctcgt tttccgcggc tagctcggcc gcctcacgat
    25921 tgccgagaaa gccggctcgc cggatatcca gttgagtcac gtcgatcgtg cgcggcttca
    25981 aatacggccg caattcgtcg gcggaacgca tattttcgcc gtcggcgatc agcgtcttca
    26041 tgccggcttg ttgcatcttc tcgcgcaaac gggcatatag ttcggggtct tcggggaaaa
    26101 tctcctcaat ccagaacagg ttcgacttct gcgtctcgct caaaaagcgc caggccaact
    26161 ggaggcggcc gcgatagccg ttgttggcgt cggccatgat cttcacctcg ggaccgaccg
    26221 cccgccgcac cgcattgacc acttcaatat cgcgcagaac tccggcaacg ccgggcatcc
    26281 acttgtcgca gcggcccact ttcagcttca agccacggta gccggagcgg accgcctctt
    26341 ccgcctccgc gaccaccgcc ccaacgcctt tctcggggat cattacatca ctgaaataga
    26401 gcgtgccgtc gtaaacctct acctgctccc gcgccgaagg accgatcaat tcatggcagg
    26461 gcacattgag aatctggccg acgaggtcga aaaggacgct gtccagaaag cggtacttcg
    26521 ccaaagccgg attccgcacc gtaaggatac ggccctggcg gatctcgtag agttccagcg
    26581 ggtcggcgcc gatcaggttc cggagcgatt cgagcattgc cttgtcggga ctggaataat
    26641 cgagcgtgcc cgtgccagaa accccctcag cggtaaatac gcgcagcaaa tgcgtggcgt
    26701 aggtctcgcc tttgggagcc tggtcgtaag cgttcatggc cacctttttg tggaagcggc
    26761 cttcgattgg cgtgattgta atgcggctga tcttggctga tcctttcgca gccggcatct
    26821 tcgcggctgc cgcggctgcg aggcacagaa aatctcgacg gcgcataaag acctcgttga
    26881 gtacctgcaa acaggtccgg cggctctcag aatctgcgcc tggaaaacac aggagagccg
    26941 ttcggccgca acccatgata atcagctccg cccggtcctg caaggctcgc tcaggccgag
    27001 gcacggatgg ccgaaacgga gatcaagagg gggctcagac gcgggcgaga tcccgcactg
    27061 cctggtctac gcgatggagc gcttcttcga tgttggccag cgaggtggcg tagctgaacc
    27121 gcagataccc ctccccgtaa ctgccgaagg acgtgccgct caaaccggcc acgccggcgt
    27181 tctccaaaag gtgggtttcc agttccgccg acgacatgcc ggtccccgtg atgttcggaa
    27241 aggcgtaaaa cgcgccgccg gggagggcgc agcggaaacc gggaatccgg ttcaggccgg
    27301 cgacgatgac atcgcggcgc cgccggaatt cctcgaccat gcggtcgacg ctctcctgag
    27361 gcccgcgcag cgcctcaagc gcagcacgct gcgtgaagct ggccgtgcag gagttggagt
    27421 tgaccatcaa ccgcgtaacg gcttgcgcca gccgctccgg catgacgcca taaccgatcc
    27481 tccagccagt catggcgtag gttttagaaa aaccatcgag aaggatggtc ttttccatca
    27541 ttccttcgat ggaagcgatg gaataatgct gctctccgta gaggatccgg ctgtaaattt
    27601 catcggaaag cacaaccaga tcgcgatcgc ggaccatggc ggcgatctcg cgcaggtctt
    27661 cttcgggaat cacgcccccg gtgggattct gcggcgagtt gagaatcagc agtttggttc
    27721 gcggcgagag ccgttccgac agcatgttca gatcgaggga aaagccgcga ctttctacca
    27781 gcggcagcgg cacggggata gccccgagat agcggatcat cgattcgtaa ataggaaagc
    27841 cgggatttgg atacagcgct tcgtcgccgg cgtcaagcag cgccagcatc gagaagaaga
    27901 ggatcggctt gccgccggga acgacacaca cgtgctccgg gccaacgcga acgcggcgcg
    27961 tggccgaaac catgccggcg atttcttcgc gcaggtcagg atagccctgg gaagggccgt
    28021 aatgggtcca cccctcatcc agcgcttttt tggctgcttc cacgatgtgg ggaggggtgg
    28081 ggaagtcggg ctcgccgatt tccatgtgga tcacgctccg gccttcggcc tccaaacgcc
    28141 gggccttgac caaaacatca aacgccgatt cggttcccag gttctgcatt cgctgcgcga
    28201 gtttcattgg caccgccgag atggaatcat gctgctgaaa agaaatgcac gccgccgcgt
    28261 tcctcgacca gcgcgacatc atcaccaata tctaccctgg ctcccaggat gcggcctgtc
    28321 aggcgaacac cgccctcgag ttcaatgata gcaatctgaa agggcaaatc tttttcgaaa
    28381 ggctcggcgg cggcgtagag cgtggtacac gaatagattt ttcctttgcc cgagacggtc
    28441 gccggcagcc atttccgatt tccgcagccg caaaacgccg gctgggtcac ggccaacttg
    28501 ccgcagcttg tacagcggta aagagtcatc ttgcgccttg cgcctcgggg ccggaaacgg
    28561 cgccaggccg ctttccgctc gcgaggcgga gtaatagagg cgcgcgccac ccgccgcctc
    28621 cgcgccagca agcctttgaa agacgtttcg ataaacgctt tctccgctcg gcaggaaccg
    28681 cgattcgaag gcgggcccgg ctcaccggcc caggatcgta actacgcaag tggcgcccga
    28741 tccaccaaga ttttgagcca agccggtctc cgcccgccgc acttgccgtt cgccggcctc
    28801 gcagcgcatc tgcaacacca ggtcgcaaag ctggccgatg cccgtagctc cgaccggatg
    28861 gccctttgcc ttgagaccgc cgctggtgtt gaccggaagc tctccgtccg gagcagtaaa
    28921 gccggaggct gaagcggggc cgccttcgcc gcgaggaaca aagccgagat cctccagcgc
    28981 gatgatttcg gcgatcgtaa agcagtcgtg caattcggcc acgtggatgt ctcgagggcg
    29041 cagtcctgcc atctggaagg ctttctcccc cgctcgttgg acggaccgaa aggtggtgat
    29101 atcgtctttg tcggcgaggg cgaggcggtc ggaggcctgt gcgacgccaa ggatgcggat
    29161 cggcttcgag ctgaaatccg gggcacgccc ggcggggcaa atcagcacgg ctgccgcccc
    29221 atcgctcacc agcgagcagt cataaatcgt aagcggatcg gctatcatcc ggccgtttag
    29281 cgcctgctct ttggtgatga gcttgcgcat atgagcgtcg ggattcttcg ccccgttggc
    29341 atgatttttc acggccaccg ttgcaagctg gtcgcgggtg gtgccgaact ggtacatatg
    29401 ccggcgggca atcatggcga acatggcggg aaaagtcgcg ccggctttta cttctgcttc
    29461 caggtctccc gcggaagcca ggatttgagt gacccgcgtt ccaggcacgg aggtcatttt
    29521 ttccacaccg gcagccagga caacgtcata catcccgccg gccacagccg cacacgcgtg
    29581 aaaaaaagcc gagccgctcg acgcgcaggc atcctcgatg cgcgtcgccg gaatcccgtc
    29641 catgccgagc gctgaagcca cataaggcgc cagatgattc tgtccgacaa aagacgggcc
    29701 agcgaagttt cccaggtaaa acgcttctac ctgcggcggc gttacatgcc cgtctttcaa
    29761 acacttgctc cccgcctcga cggccaggtc gagcagggaa cggtccggaa acgcgccgaa
    29821 cgccgtcttg cccacgccga tgatggaaac aggcctcatg tatactgaaa tgatcggaga
    29881 ggcgctgatt tgcgggttcc gaacattatt attccacgtt ttggcggcgc aggcgtcaag
    29941 ctcaatgtag ggcagtcatg cgatgaacac cgcaggcgcc gccgtttccg agacctaccc
    30001 cgcgttccag cggcagggga gcatggagac cgataaggtt ccccggccgg ccgacgtctt
    30061 gcaggctgcg gagcaggatt ttatcgcggt gcatctgcgg cgggtgtttc tgctgattca
    30121 caggattgtc ggcaacgtcg ccgatgcgca ggacctcacg caggaagctt ttgtcaaagc
    30181 gctggcgcgg cgcgatcagc tcaaagacga acaaaaggcc gcccagtggt tgggccgcat
    30241 cgctttcaat acagccatcg atttcatgag acagcggaag cgggtcagct tctgtgagct
    30301 ggaggcggct ccagagccgc aggttttcga gacgccggag cggctggtgc tgcgggcgga
    30361 gaaacaagcg tacctggccg acggactgcg gctgctttcc gagcgggaaa gggccgcgct
    30421 catattgcgc gacgtggaag ggctgccggc ggcggaagtg gcccaacata ttggctgctc
    30481 gatggcgact gtgcggagcc acattgccaa tgcgcgaacg aaattccgca agtatattaa
    30541 aggccgcagg gcctgaaccc tgtccggccg gcgtgcgccg gcgcgctcac gttcgcgcaa
    30601 gaagcgcgcg ggcggctgcc ggagaaagac cgggaaacaa gccatgagca ctttattttg
    30661 gaagcacccg ggagaggccg acctggcgct gtttgccggc ggtgaactgg gtctgctggc
    30721 ccggtggcgc atcgaagggc atttgcacgc gtgtggagag tgccgtcaag ccgtgggcga
    30781 gttcttcgag cttcgcagcc gggtgatgga cctggcagaa ctgccgcaga tggattggaa
    30841 tggcctggca gctcagatcc agcggcgtgt ggagcaacag cgtaggatgt catcgtcctt
    30901 cgcgccggct cgccgcgcct ggcgtccggc atgggctccc gcccttgcgg ttctgttgat
    30961 gctcgctgcc ggcgcatata tcacccggcg ctaccgcgcg ccggagccgc cgctatccgg
    31021 agccgcctcg ctgaacgcct cggccggggc ggtcgaactg cgcctgggag accggcaggt
    31081 cttgacgctg acgaacgacg cccaggcgga aagccaggtg aactggcgcg tgagcgccgg
    31141 cgccgtgagc gcccggtatc tggatgccga taccggaaat atcacagtaa acaatgtcta
    31201 tacgcaatag catcgtttac gtttctttcg cctgttggtt ttttgccgcg gcggcgcaag
    31261 cgcagttggg gcaacggacg gatccggctt cggtgatccg ctggagcctc ggcggcgcgc
    31321 cggtttcgat cgtctccgcc gatctcggcg actcccgcgt tcagccgcgg ggcggcgcta
    31381 tgattttgga cctgcatgcc acgctgactt tgcagaacaa cagctcgcaa aatattcgag
    31441 gcatcacgct ggcggttctc gcacaggaga tgacgccggg cggcaaagga tcggtcgccg
    31501 ttcccagctt gaatgtcccg cctggcgcca gcttccccgt gcgcatcaac ctgcgccttc
    31561 tgcgcccgct gccagcgccc gccgggccct tggtcgaagt cagccttgac ggcttgctgt
    31621 tcgccgattt ctccttcttt ggcccgaatc ggctggaatc gcggcggacc atgaccgttt
    31681 gggaaatgga ggcgcggcgc gatcgcgagc atctgaaaac gctgcttgag aaacagggtc
    31741 cggccgcctt gcaaagggaa atgctggcga gcctgtcgcg gcaagcggct cggccgcacc
    31801 tggatgttca ggtcgagcgg gccagcggct ctcgggcgat ttcagccgcc gtgagcgccc
    31861 tgaccgggcg aaacctgaat ttcgcttttt taagaattcc cgattcaccg ctcgatctgc
    31921 tctcgggcgc ggcgcaggtt aatggggccg aagccgcttc tctccgcgtg gatgtcgcca
    31981 accgttccaa ccgtgatgtg cgctactttg aagtgggctg gattgtaaaa gatgccgccg
    32041 gtgagcagtt tctggccggg tcgctaccat cgtcgcccgc gggcctcgac ttgcggccgg
    32101 gcggttcggc cgttgcgttg caggaccgcg cctaccggtt cagccgcggc cctgagtcgc
    32161 tccccgctat catcggtctg accggttttg tgagccaggt cgaattttcg gatggcgcca
    32221 tttggatccc cagccgcaag gctctcaccg agcagtcgct gctgggagtg atccccgttt
    32281 ccgccgaaga gcagcgcctg agccagcttt accggaccaa aggactgcaa gctttggttg
    32341 aggaactgcg gaagttttag gagcgccgcc ccgcctgcac ctcagcgagc aggcgcaacc
    32401 cgcccggctg tggtcgcgga gaccccttcg ccgagaattt cgttcagcag ccggctgtcg
    32461 ggctccacag ggtactgaat tcccagcatt ttggccagcg tcggagcaat atcttcgggg
    32521 ccggccgggg ctgggtagcg ccccggcttg attcccgccc ccatccaaac aatcggcacg
    32581 tggcgatcgt actcatatac agtcccgtgg ccggtcccgc cggggtttcc cgatatgtaa
    32641 tggtattgct tggcaacgac catcaaatgc gggctgcgcg gcgcgaagaa actgttgcgc
    32701 agcagggcca gggcaggatc ggcggaggaa ggcttgtcgg aaatcatatc ggcccaggtg
    32761 taaacagcgg cgacggctcc ggtggccatc agcgcgcgga tcgccgtttg ctccacctcg
    32821 ctgcgtttca agccgcttcg cgcgatcgca tcgagattca gatgaaaatt cgggccttcc
    32881 ataccggcga tcaattccgg cgcggcgggg aattgctgcc gaaacgctgt acgcactgcg
    32941 ttcaggagaa gcgacgtagg cacgcgcttc gcctcgctgt tgccttgctt ttgcagccac
    33001 tcgaccagcg gcagcgcccc gtgatccgcg gtaagtatga ccaacgtgtt gtttaggccg
    33061 acgcgcgcgt cgaccgcgtg gaagagcttg tcgagcacga ggtcaaggcg caaatagttg
    33121 tccatcgctt cctggctgaa ggggccgtag gcatggccga tgtaatcgct cgccgagaag
    33181 ccgatgacaa aaacgtccgg agcggcatcg gtgcccagat tgtgggctgc gagcgcttcg
    33241 agtgccgctt gcagcagcac ttcatccgca tagggggtgc gccgcaggct ttcatagtag
    33301 gccggctccg gcggcttgcc tcgatagccg tgcgggaagg tcgtatcttt gagatcccat
    33361 tccccgggaa aattgtcctc tcgagaatac tggagatagg ggctttcgtc tgagagcaag
    33421 cgggtccatg gcgtttggta gtaggaatcg ggaagcttct gttcgttgaa ttttgtcagc
    33481 cacgcagggg tctgccgagt gtagtacgtg ctggtgatga aacagccgca gtcgttttca
    33541 aaccaatacg ccgcatcggc gcgagggccg ctgggcaata tcgccgaacg gtctttgaag
    33601 gaaatgccga cgacatggga agcgggccat ttctgcttga ttttgtctcc caccgtaaaa
    33661 ccgacgaaat tggcgggcga agcgccatgg ccgcggccgc cgacggcgtc cacggtcgaa
    33721 tcatccacca cattcatcat tgttttcagg taaggatcgt accatccgtt cgcgactatg
    33781 ccggagtgag agggatggcg cccggagaga atcaccgaat ggccggggcc tgtctcggag
    33841 ttggcatgac gatatagcgc attagtgaaa atggccccgc ggctcaagag cgtcttaaaa
    33901 ccgcctttgt aaagaggctc aaagcgggtc agataatcaa accgcatttg atccaccgag
    33961 acgacgagaa tcagcttggg tggggggggt tgggcagtga caatcgcaga gattgccaga
    34021 aatatcgtca gcaaccgagc tttagggcgc atcacgacag ctcctggagt ggaaactact
    34081 acagtctaca ccggttcttt gacgcgagaa cgattgactc caccgggcag ggcattggga
    34141 caggctcaac gccgggcagg acggcggcgg actgcggcgg ctgccacaga ccttcgggac
    34201 ttctccgggc cgccgggctg ctctccgggc ggccgcgctt gaccgcgccg ccgcaaaact
    34261 gcgaaattga gtgcgaggtt ttcccatggc gaaattcctc ttgggactga tcactggcat
    34321 tttcctttgc gtatttcttg ccgtgctggt cgtggtggcc gctgcctcgt tttccggccg
    34381 cgcgccgtcg gtcagccagg gatctacttt agtgctcaac ctgggcgggg agattgtcga
    34441 gcacaatcca gccaatgtgc cgtctctgct gttccagcga ggcatgaagc cgaccgtgaa
    34501 agacatccac gacatcctgc aaaaagccgc cgtggaccgc cgggtgaatg cggtggtgct
    34561 gaaggctcga ggcatcggcg ccggctgggg aaaaatccaa gagattcgcg cggatctgga
    34621 gcagttcagg aattcccaaa agccgttgat cgcgttcctg gaaacgccgg gcgctcggga
    34681 atactatctt gctacggcct gcgacaagat ctatgtcgcg ccgcagggtc tcctcgacgt
    34741 gaagggcttt cgggcggaat ctgttttctt taaagacacg ttaagcaagc tcggcgtcca
    34801 agcggactgg gtgcatgtcg gaaaatacaa gaacttcgcc gagcctttca cgcaggacaa
    34861 aatgtccgac gcgacgcgcg aggtgatcaa ctcgattctc gatacggtcc tgaacgattt
    34921 tgtcgctacg gtcgcaaagg gcaggcacat gacccccgaa caggtgcggg ctgccctgga
    34981 ccagggccct ttcctgcccg aagaggcagt gaaggccggg ctggccgacg gcctgcgcta
    35041 tgaggatcag gtcttcgacg aagtaaagaa cctgaccaag gtcagcgcca tcaagaaact
    35101 tcgagcggaa agttacagcc gggtcagcct ggagtcgctc ggcctgagcg gcggcagccg
    35161 catcgctgta gtctatgccg tcggcgatat tcttcagggt gagccggagg tcagcccgtt
    35221 tggaggcggc gaagcgatcg gctccgacac gatggcccgc gtcctgcgcg acgtggggga
    35281 agacaaaaac atcaagggcg tcattgtacg catcgactcg cccggcggtg acgcgttcgc
    35341 cagcgatgaa atctggcgct ctatgaatct gctgcattcg aaaaaaccga tggtaatctc
    35401 catgtcagac gtggctgcct cgggcggcta ctacatggcg atgtccggag acccgatcat
    35461 cgcctatccc gggacctaca caggctcgat cggcgtggtg ttcggcaaat tcaacctgca
    35521 cgggctgtac gacaagctcg gagtgcggaa ggagatcctg agccgcggca aattcgccga
    35581 tatcgatacc gattaccgcg atttcactcc ggaagagatg caaaaagtca tgagcggcat
    35641 gaactcggtt tacgccgact tcgtccaaaa ggtggccgag gcgcgtaagc ggaaggtgag
    35701 cgacatcgag ccgatcgccg agggccgcgt ttggctcggc agccaagcca agcagaacgg
    35761 cctcatcgac gagttgggcg ggttcgaccg ggcgatcacc ctgatcaaag agcgcgccaa
    35821 gctccggccg gatgataagg tgacgctggt cagctatccg ccggaaaaga agctcctgga
    35881 cgtgcttctg tcacggttga acggcgcagg cgacgaagat acgctcaccg agaccatccg
    35941 ccgaaagctt gggtgggcgg ctccctggcg ggctctgctc aaggggggca tgctgaaggt
    36001 cgcgccgtat atcgtcagcg tacagtgagc acctggaaaa agccgatcgc tctttggctg
    36061 ctgagtctgg gagcagcagc ggccttcgcc gccgaagttg atttgcgccg cgccgttgtc
    36121 gtcgcgccgc cgcagctttc cccacgagaa agcagagccg tcgccatgct ggtggaggaa
    36181 gtcgaaaagc ggacccgcat ccgctggccc gtggctgcgt cgcggccttc cggctcagtt
    36241 cccgtaatct ggatcgagcg cccatcggga gcaggcccgg cagagggcta tcggattcga
    36301 gtggatgact ccgccgcggc tccggcggtg ttcatcagcg gcaacgatgc gcgcggcctt
    36361 ctgttcgccg tcgggtatct gctgcggagc atgcacatga gcgcggagaa ggtcacggtc
    36421 gccgccgcgc tgcacgtctc ggctgcgccg aagtatcctt tgcgcggcca ccagctcggc
    36481 taccggccaa aaacgaactc ctacgatggc tggtcgctgc ctatgtggga gcaatatatc
    36541 cgggacctgg cggtcttcgg cgccaatgcc atcgagctga ttccccctcg ctccgacgac
    36601 gacgcctgga gtccccattt cccgctgccg ccgatggaga cgatggtcgg catgtcgcgc
    36661 ttggccgacg aatacggcct ggacgtctgg atctggtatc cggccatgga caaggactac
    36721 gcggatccca agaccgttgc gttcgctctg tatgaatggg gtgaggtttt ccggaaattg
    36781 ccccgcattg atgcggtttt cgttccgggc ggcgatccgg gacacacgga gccgaaatat
    36841 ctgatggcgc tgctggaaaa gcagacgcag aatttgcatc gctatcaccc caaggcccag
    36901 atgtgggtgt cgccgcagag cttcagtcag gcctggctcg atgagtttct gggcatcctg
    36961 agaacgcaac agccgccgtg gctggccggc gtcgtcttcg ggccgcaaat tcgaatgagc
    37021 ctggcgcaat tgcgagccgc tgttcccgcg cgatatccga tccggcatta cccggacata
    37081 acgcacagcc ggcaatcgca atatccggtt caggattggg atatggcgta tgcggcaacc
    37141 gagggacgcg aggttatcaa tccgcggccg ctcgacgaag cccgcatctt ccgcctgctc
    37201 cagcggtata cgatcggctt catcacttac tcggagggct gcaacgatga cgtgaataaa
    37261 gctgtctgga gcgctctcgg gtggaatccg gaggccgatg taaccgatat tctccggcag
    37321 tatagccgct atttcctcgg agatcgttat gccgatgact ttgcacaagg cctgctggcg
    37381 ctcgaaagaa actggcaggg gccgttgctc gccaaccaaa acgttgagac gacgctccgg
    37441 caattccagt ccatggagcg cgccgcatct ccacaggatc tgctgaactg gcgttttcag
    37501 caggcgctct accgcgctta ctacgacgct tacattcggg cccggctggt ggcggaaacc
    37561 ggcgacgagc aagcggccat tgcccggctg gcggaggcgc cgcggaccgg ttcgctttcc
    37621 gccatggcgg aagcggaagc cattctggac cgcgccgccg agcggaggcc gtttttcgcg
    37681 cttcgcgacc gcgttttcat gctggccgaa gcgctgtttc aaagtattcg catgcaatta
    37741 agcgtgcccc tgtataaagc gattgccgtc gggcgcggcg ccaatctcga taccatcgat
    37801 atgccgctga acgaccggct ttggctgaag gcgcgcttcg ctgagctcag aagaatgccc
    37861 gaggaatcag accggctgaa cggcatcgat gcgatcgtca actggaccaa tcccggtccc
    37921 gggggattct atgacgattt gggcgatctg gcgcggcaac ctcacctggt ccgcggaccg
    37981 gttttcgaac aagatcccgc ctttcttcaa tcttctctcg tctcgttctc ctaccgcccg
    38041 gagtaccgga aatcgtggtg gacttatgca gaatctctga acgatactcc cctcgagatg
    38101 cgctacagag accttgaccc cgcggcgcac tacaaggtcc gggtggtcta tgctggggat
    38161 agtccgaata cgcggattcg gctggtcgcg aacggcaatg tggaagtgca tccgctttta
    38221 cagaaacctt cgccggtgcg gccaatcgaa ttcgatatcc ccgccgaggc gaccgcctcc
    38281 ggcgagctcg ttttgcaatg gcgccgcgaa ccggcgctgg gcggcaacgg ccgcggctgc
    38341 caggtggccg aggtgtggct gatcaagaag agccaataac aaatcgtccg gcaggcgcgc
    38401 ggcaggccgg tttgtcattt agagtttgcc aaaggccgtg gcgtcccagg tagctgccgc
    38461 aaggacctct acgttcgtcg ctttctcata aatgcggcga accacagcgg gttccatcgt
    38521 ctgttccgag ccatcgacgg cgcctgccag cacgatgcgg cgcggagcga gggaagcggc
    38581 cacctccggc aggtcggtgt attccagcac atgcggcaag aaattgcaaa atgggtgcgt
    38641 gtactgctcc gtctccacga tgctccgata agaaacgagg cctcccgcca agtagaggga
    38701 ttgcaccgca ggttcaaggg cggcagcgaa cagagccggc actgtcattt tacccagcgc
    38761 ggcgattgtg agccgccggc cggcgagaga gggatgcgag cgcagcgcgt ggacgacggc
    38821 caggatatcg gttacacgct ggccgagcag cggtttgccg agaatgagcg acgcccaggc
    38881 ccaattgtct tcgttgctgt gtgagcgcgc ataacgcgcg gctccgcggc tgaactcggg
    38941 agaaaggtcg ccaacgccgc gcaggtcagg cacacacacc gcgcagccgg cctgcgccag
    39001 cgactgatac atctcgccct cgtgccagcg cacatcccgg ccggcgggct ccagggatat
    39061 gacggcaggc tgagaaggat ccggattatt cggcagaaag agccatgccg gcgcccagac
    39121 tttcggggcc gaaggaatat ccaggacttc aatttcaagg ccacgcgagg ggacccgccg
    39181 caggacggcg gcgcggacct cgcccgctgg ccgctctacc ccgagcaggc ggtctaacgg
    39241 cgccgaacgc cgggtgagct gccgggacct ggtcaaggtg aaaggcgttt gcccgtgaaa
    39301 cgagcgcacc acgttgcccg attcgcttac cgcaagaact ttgtcgggct cgggctcaac
    39361 cggaggctcc tcggtgatcg ggcgcgtctc tcccttcagc cagcggccga gccagttgta
    39421 aacgtgcaag cgagagtcgt aggaaaggcc gtgcggaagc ggggtatccg cccaagccag
    39481 ccggtcgccg cggcccaaaa cctcgtatac tcttttcagc ttctgaaatt cctcccagcc
    39541 gctggctatg tactggctag agtatgtgcc gaaaaagtcg cggtcgctga ccgtgaccat
    39601 gaggggcttg ggggcgaacg gatacagcag atcccaacgg tcaaagccct ccggcccgga
    39661 gccgacgaag tcctgctccg cgtcatcggt ggatcccggc gggttgaaat tggcgcaggc
    39721 aaagttctcg gtattgccgc tgcactcgat ggccgcagcc agccggtcgt ccacggccag
    39781 cagcatcatg gtgagggtcc cgccgccgga ctggccggtg gaagccagcc gtttcgggtc
    39841 gacgagcggg tgcgctgcga ggtagtcaag gctgcggacg gcgtcccaga cctggaagcg
    39901 cgaggccgta tcgcccacca gcagcatttg gcggccggcc gtcgtgtgtt cgtcgtccgg
    39961 agaaaaaagg cgggtgcggg tcagagacgc gttgggatag tagatgcgct cgccctgtcc
    40021 catcgggtcg aaggccaaga ccacgtatcc cagccgggcg aggccttgac agcatcgctg
    40081 ataggtgtct cctgctttgc cgttcgccgt atggcccatc tggaaaagca cgcccggata
    40141 cggcgggcgg cctgtagtgg ggatgtataa attggccgag acgtgcaatt cggggcggct
    40201 ctcgtacacc accttctcga cgcggtagcc gggccgctcg aaggatccca cggtgcgggc
    40261 gttcagggga gtggattcgg gtcggccgcc aaccaggcgc cagaacgttg cgcgcgccca
    40321 tcgctggcgt tctcgaatcg cctgcggcgt ggttagtttg gcgatctcgc ggtttctcgc
    40381 ttcataagcc cgcgccgcga gatcgcgcaa ataatcggga aggcagcgcg agtagttgcg
    40441 atacgccact ccggggaatg tggattgagc cccggccggc ttccgggcaa acgcccagac
    40501 tgccgggatg gcgaataact cgcgcctggt gagccgaaga gaatggtccg gcatgctccc
    40561 gcctcgcccg caaacctgcg agtatagcag cccttccgta aaagcgacct ccggatctac
    40621 gattcggacc gcggctcgac gatccgcgtg gcgacgtgca tgcccggctt gagctgatcg
    40681 gactccacgg cggtgaagac ataatccgtc aatatgggct ttacccgcac gggccggaat
    40741 ttgttttcgc cttccacgac ccaaaccatg ggatcgccgg gcttgagctg attggcatcg
    40801 agaagagctt gtttttcctg ttccggaatg gcgggcgaaa accgcaatgc gaagctcggc
    40861 accttcatct tgttggtcac ggagttcacc ggaatggtta cgtaggccgt catcccggga
    40921 ataatcctct ccccgggatt gcggaactcg atcaaggcat cgtaggtcac taccgtgttg
    40981 cgagtcgtat ccggcggaac gttggatcca atcaagccga tgctgaggcc tgaagggttg
    41041 gtgtttgtgc tcgtggcggc ggtggtggcg ggggtaatcg tcggcgtcga agtcgtagtg
    41101 atgattcggc cgctcggatc aaatgtcgtg gtggtagtgg tggtgatgtt ggtggcctgg
    41161 gaggccgaca ccgccgcgcc gcccacggat tcgctggccg aaaccgaacc ggcggttaca
    41221 ccggccgaaa gcgtcttttt aaagacgctg gtcagtcccg tggtcttcga aatcgggtcg
    41281 atttcgttgg cattgatgcg aacggaaacc agttttcctt cgaagatctc gcccggaaaa
    41341 gcgttgaccg tgaaagtggc cggcaagccc actcggacct tgcccacatc ggaggaatcc
    41401 agcttcgtgt acacgtacat gtgggaaaga tcctgggcga tgttaaacag actaggcgcc
    41461 tgaagactgg ccgctacggt ttgcccaacg tcgacattcc gagcgacgac aactccgtcc
    41521 accggcgagg taatccggca ataatcgaga ttgcgtttcg ccatggcggc catcgcggcg
    41581 cgctgttcga ctacggcttg cgcctgggcg agggccgcct cggcgctttt cactctggcc
    41641 gcagcgacct ggctttgcgc ctgggccgcc agcgcatccg cccgagcact gcggtatgtg
    41701 gcctcactct tatcaaaatc ggcctgggtg gaaatacctt cgctgagcag gcgcttcatc
    41761 cgattgaaat cggcttccgc ctgccggtaa gtcgcctcgg cggcctgggc ctcggcggtc
    41821 gctcgggcat aatcgctgcg cgccgactcc acattgaatc tctcctgatt gacggccgcg
    41881 cgggccgccg ccagattggc ctcggcttgc aggaacgttg cccggtacgt ggaatcgtcg
    41941 atctcggcca gcaattggcc ggccttcaca cgatcgttaa aatcaacgtg gagggccttg
    42001 atgcggccgg tcaccgggct gccgacatcg accacgttaa cggcgtccac gctgcccgta
    42061 gccgggactt ccaccgtcag gtcgccgacc tccaccggcg ctgtctcgag aatgggtttt
    42121 tcgggccctt tcgagctcag ggaccaataa agggctgaaa cacaaatcgc gacgcccagg
    42181 gcgatccaca gtaagttctg ccttttgcct gacttgttca ttgagtattc cggccgatta
    42241 tacctggaaa ggcgtgccgc cttctgtagc ggttacctgc gcgccggaat ttcccttcgt
    42301 gcggccctaa acgtcgccga ttccggctct tcccatcttg cggcggcggg ccggcgggct
    42361 atactaacgg atggcttatg ctcctcgccc tgatcttgat cgctggcctc accgattggg
    42421 ttccggcccg ctggatctcc agcgatccga aaagcctgga actgatacgg ggcacgccca
    42481 ttaattgtct cctgctggaa cgcgatcagt ggtcgcccgc gttcgccgag gaggccgccc
    42541 ggcagaacgt ggtgacgctc ggcgtcgtgc gcccgggcgg agatccgctg gaatcggctt
    42601 tggcggcgct gcgcgagaag ctccagggcg tggtgctgga gggggatttc ccggccgcgg
    42661 agatcgcgcg cgtcaagcag aagctggcag cggcccgcgc gccggtagtg gaaatgctac
    42721 ccaggcgttc gctggacctg catggacagg cagacattat tgcgacttat caggcagtct
    42781 ggccgggcat tcaggcgcag gatgacgagg gcaaagcgcg ggccggaccg accggttcgc
    42841 cctggatcaa caccaacacc ggattcctca tctacgcacg ggcggcggcc gagggcccgg
    42901 tctgggttgg caatttgccg ccggagaaac aggtcctgac ggtggaaaat tacctctacg
    42961 ccgtctgcga cgccgcttct ttgggcgccc gctgggtagt ggcgctcgat ccggatttcc
    43021 gcggccgggt tttgcggcgc gaggaaaagg cgctccggga ttggaaacgc atcgcgcttt
    43081 atctccaata tttcgaggac cacaaggaat ggcgcgcgct gcggtcgcac agccgcctca
    43141 cggttctgca agacgccgac agcggagcgc tgctttccgg cagcatcctc gacatgatga
    43201 tcaccaagcg cacgccggtg cgccttctgc cccgctccaa gctgaacgaa ggcgcgctgc
    43261 ggggcgccac catggtgctg aacctcgaat ccgatctcct taccccgcag cagaaggaga
    43321 ctctggcggc atttacccat tcggggaaaa cgctacttac cagcccgccc gagttcaaac
    43381 cgcccgtgct gcacggcgat caaattacgg tggatgagaa agaggtgggg cggatcgaag
    43441 acctatggcg cggcgtcaac tcgtccatcg cgcacgcgaa cctcggcctg cgcatgtaca
    43501 acgtgtccag tatgctcacc aacctgctag cggattcgac gggcaagcgg ctcgtgctgc
    43561 atctggtgaa ctattccggt tatccggtcg aaaacatcac tatcttttac ccggggagcc
    43621 tcaagagcgc gaaaattctg acgcccgaaa agccggctca ggaggtcgag attttcgaac
    43681 agagaggcga gaccacgatc gaactggaca gagtaggaac ctgcactacg ctcgtcctgg
    43741 aacaggaggg ttgaaatgcc tgagatgaca cgccgacaat tgcttacgat gctgggagcg
    43801 gcagcggctt ggccggttca gaaaagctgg ggtgctccgg aggcgccggc cgccccggtc
    43861 tccatcgcgc gttgcaagtc ttacgatgaa agccttgtgg caacgctctc caccatgttt
    43921 gaccagttgg gaggcttgca gaaattagtg aaaggcaaga cggtcaccgt caagctgaac
    43981 cttaccggcg acccaggcct tcggctccag ggcaagccgg tcggttcgac gcactataca
    44041 catcccacac tgatcgaagc gtttctccac ctcgccgacc aggccggcgc gaagcgcatc
    44101 cgcctggtgg aaagctgctg ggcgacggca gggccgctcg aagaatacat gctggattcc
    44161 ggctggcgcg tgcggcggtt ccaaagcgcc gccaagaatg tggagtttga aaacaccaac
    44221 gcgctcggca aaggcaagca atacagccgg ttcaaggttc cgggacaggc ctacatgttt
    44281 ccggcgtatg acctgaacca ctcctacgcg gataccgacg tctttgtgag catggcgaag
    44341 ctgaaaaatc acgccacttg cggcgtcacc ttatcgctga aaaactgctt cggaatgacg
    44401 cccgcctcga tctacggcga ggatgccggc gagtttgagc cgaacgaatc gccgggatcg
    44461 gggcggggcg cggtttgcca ttacggcaag cgcggcccct ccaaatgctc gccgcaagag
    44521 ctgcacccgg agagcccgcg cgagggcggc taccgcattc cgagaatcgt cgccgacctg
    44581 accgccgcgc ggcctatcga tctggcgatc atcgacggca tcgagaccat gatcgccggc
    44641 gaaggtccct gggtgcgagg gacggccccg gcccagccag ggcttttgct ggctggcttg
    44701 aacccggtat gcaccgacgc cgtagccacg gccgtcatgg gctatgatcc gagggccgac
    44761 cgcggctcgg cgccttttga aaaatgcgac aacaccctga agctagccga agctctcggc
    44821 gccggcacaa ccgacctcaa gcgcattgaa gtgttgggag tgccaattga gcaggccctg
    44881 tttccctttc ccaaacactt gaacatgaca ggctgacgcc gtcccggcca tgcgcccgga
    44941 gcggccttca gattcggttc agaaactgtc ccggcggggc tgcaagcccg gcgtttgcgc
    45001 gccggcggcg gggctggacg ctgcggccaa ttctgatttc ttatatttcg gctcgctcaa
    45061 atagccggac acctcgttct tggtgatttt gttgaccacc aactcaagcg gctgcggccg
    45121 gtcgctgaga ttgatcagaa tcggctcgta gagattcacg ccttttttct ggagctcgac
    45181 gtcgtccaca attagggcca ggtcgtagga cttgcgcttg gcgttggtct tgcgcagaga
    45241 aaggctcaga gggcctactc gctggaactg cttggatttg gtgatatgga attcatcata
    45301 atttcgctcg ccgcgtttct cgagcagcac gagctcctca tgatttctgg cgattgaacc
    45361 gctgaggtca tcgcgcgtgg agttcagctt gttttccata tcctcgcgcg tctggctgag
    45421 cgctttctgc gtgcttgcga tctgctcgcc ttgccgcgag agctgcgcct gcatctgcct
    45481 ccagcgggcg tcctctagcg ggcgggcctg ccttgttctg cccgcgcttg cgccgcccgg
    45541 cttgcgtgcg gctttggcgg gatgcgccgg cggggcctcc ctcgaagcat ccagcgcatc
    45601 cagcttggcg cgcagcgcct gaatctcggc ctgactttgg gccagagcgg aggtcatctg
    45661 atcgcggctg gcggccagct cgcgctgttg ctggcggtcg cgccaggctt cccgcacgcc
    45721 gagaccgcct atgcaggtga acaccaacac ccatgccagt aaattcaaag gccctctggc
    45781 cgggtgcacc ggcgccggct cgccggacgg cggtcttacc tcggagttcg ggacattcat
    45841 tccttccgac ataagttccc ctcccaactg ggcataaaaa ctcgcccccc tgacagcaag
    45901 caaagtgcca gcctccggtt acatccaaga ttatgaaatc acgagacttc tccctagcca
    45961 gagatccgcc ggtttcgcgc cgggaggaag acattacatg ggggttccgc aaaaacgccg
    46021 ggggtggaga tcggctgggg tttccttttc ggctgctcta cctctgcgcc gccccggcgc
    46081 gacccatgat tggccagacg tcaacgatcc tctctccctg agccacgtaa taacggtcgt
    46141 agacgttggt actcatgtcg agcgtcgtgc aataaagttc cacccggtcg ccaagcttaa
    46201 gatcctggtc gccgtccttc catttgagga tgccgtattc ggcgccctgg ttggccacct
    46261 gaatccaggg catgcccttg acgttgtcgg tggggcgaag catggctttg ttgccgcagt
    46321 cgatcgtgca ttgattcggc cagcgcttgc tcgtaacggt ggcaaggacg gtcagagagc
    46381 cggcgaagtc ggaatacatg ctgtcatcat ctttgccgcc gatggcgaaa taggcggtgt
    46441 ccatgaagat ataggagccg gcctgaagct ccgtaaggcc gttctcttta tcgatgttat
    46501 aggtcccggt cgagccgccg ctgatgatct gcaccggcaa tcccgatttg cggcacagtt
    46561 ccgccgtttc gcgcaggccg gccagatccc ccgccgatct tttgcggcga gcttcccaac
    46621 cgtgggtgtg cgatgcgccg ccggagtatc ccatcaagcc ccgcagctcg agatgctttg
    46681 acgaatcgat tttttgcgcc aattcaaggg ctggctggcc gggctgcatg ccgtggcgcg
    46741 tcaggccggc gaagacgtcc gccacgattt tcagacggac gttgtgcgcg gccgctgcct
    46801 cttcgaccca gtcggcgacc aggggatcgt cgaccacgaa catgaaggtg ggatcccgtt
    46861 tggaaagggc gacggcccgc gagatgttgt tgatgcccgc aggctgcttg gtccacagaa
    46921 cgttcttgaa tcctgcgccg gacatcagtt ccgactccgc gatggtggcc gtggtgaggc
    46981 cgatcgcccc gagggcaagc tggcgcctgg cgatatccac actcttgtga atcttcacgt
    47041 gcgggcgcag gtggatgccg gctgtcttgc agtggttcgc cattttttgc agattctgct
    47101 cgaacagcgc ctgatcgata atcatgcagg gcgtgggcag ttcggccttg ctgagaccgc
    47161 gaaagtcccg ccgcgctatc cgggcctcga tgtcgttgga gtaagtgttc gtccgggccg
    47221 cgcgagcgcc ggcggttgta agcaaacttc cggcggccgc cgttttcaag aaggatcggc
    47281 gtgaagtcat ggtaggcgcc tcgctgacgc tgggtccatt atagaccccg gcgcaagagg
    47341 ctcccgggag tttggggctc cgggaacgcc gcttttcacc cagacggtgc ggttccagga
    47401 ccgtgcgtcg gagacgccaa tccgcgcata caggaccggt tcggccgcat tttccatgtg
    47461 ctttcgggag acaggctttt ctgctactct cttcagtgac gcagggaggc gaccatgcga
    47521 gttctggcgg ctggtcttct tttgacctca ttggccgcgg cggcttccac cccggcagcg
    47581 ccgacgttca gcaaggatat cgcccccatc ttgtacaagc actgcgccgg gtgccaccgt
    47641 ccggacgaca tcgcacctat gtcgctgctc gattacaagt cggcgcggcc gtgggcgaag
    47701 gccattcgcg aggccgtgct tacccgcaag atgcctccgt ggttcgccga cgcccgctac
    47761 ggtaaatttt caaacgatgc gcgcttggcc cccgaacaga tcgaggccat cgagcggtgg
    47821 gtggacagcg gagcgcccga aggcgacccg aaggacttgc cggcaactcc tctcttcacc
    47881 gagggctgga agctgggcaa gcccgacatt gtcgtcgata ttggcgaaga ctacgtggtg
    47941 aagcccggca acgacagcta tgagcacttc atcgtgccga ccaatctcca gcaaggcgtt
    48001 tggatccggg cggcggaaat caggccgggc aaccgcaaag tggtgcacca tgtccatgtc
    48061 aatctggtgc aggatgagaa gcaggcgggc cccacctcca tcgagtcgat gaagtcgctg
    48121 aacgattttc tgatccgcga gggcaagctg acccgggtgc gagaagatgc gccggttgct
    48181 gacaatggct gctccgccga tgccgccaac ttgccttata tgcgcggatt ccaggagggc
    48241 gcactggcgt cctttctgcc cggccgcgcg ccggatgttt tccccgaagg ctcggcgaaa
    48301 tgggttccgg cgaactcgaa attcgagttt gtgatccatt acgccaagat atcccgcgat
    48361 gcgcagaccg accgtaccag tgtcgggctg tatctggcgc cgaagccgcc cgaacaggcg
    48421 ctgcggcgga tggatctgcg aaatttcttt ttccgcatcc ctccggggga tcccagccac
    48481 gaagtcaccc gctgttacac ctttgaaagg gacaaggtgt tgctctccat cacaccgcac
    48541 atgcactacc gcggtaaaga cgtcacctac gatctcaccc ttcccaatgg gaaacatgag
    48601 actttgctcc tggtgcccca ctataacttc aactggcaac tcgtctaccg cttccaggag
    48661 ccggtttatg cgccgaaggg gagcgtcctt aaagtgacgg cgcattacga caacagcccg
    48721 aacaacccat ttaacccgga tcccaccaag gcaatccgct ggggcgacaa aagcgaggaa
    48781 gaaatgatga caagctggat tgaatacgtg gatggctcct ccttgcgcca gaccgcggca
    48841 tacgcgcctg cgaagtgatt tcaggcctgc ttcatccgta agttccggga gggcgagcag
    48901 gaaaaccggc cgggcaggtt gtgtcggtcc ttacaagccg cgaggaaagc gccccctggt
    48961 ataataatta gctgcccctc cgagtccggc gaccctaccg tcacccaaga tgaccatggc
    49021 tccggaccga cagggcgcgg ccggaaacgg cctcgcctcc cgtgcctgga aaggagaagg
    49081 gtggcgccag aggcctgcac gttccgcacg ggtgcaggct tttttgttcg ttccatgtcc
    49141 agcgcgatca cattcgacct ttggcatgag cgcttctcgc gcttcgccga ggacctttat
    49201 ccgggaaggt actcccggga ctatgtgcgg acgctggtcg agttgggaca gcaagtggaa
    49261 tggcttgcac gccagaagcg ctccaccatc gtcgctcata attaccagta cccggaactg
    49321 caagaggtgg cggagacagt cggcgattcg ctcggcctga gccagtatgt cgcgaaaaag
    49381 cagtcgccga gagttgattt ctgcggggtg ctgttcatgg gggaaacggc gaagaccatt
    49441 ctgggagacc gctcgaaggt ctatatgccg gaccggcccg ggtgctcgct ggtcgcctct
    49501 atcgaccagc gccggctcga tcactggctg caagccaatc cggacggggt gatcatttct
    49561 tatatcaaca ccgacgctgt gaccaaagcg cggagccatt acgtctgcac ttcgaggaac
    49621 gcgccgcaag tgctcagccg ggcggccgcc gataaccccg gccgccgcat tctgtttctg
    49681 cccgataaat acttgggagc cttcgccgct tcacaagccc gggtggatcg cagcctggtt
    49741 gatctctatg acggctcctg ccacgtgcac tccaaaatca gcgagacggc gctggaggaa
    49801 gcgattgacc tgcatccaga cgcggaactg ctgatccatc ccgaatgcgg ctgcgcctct
    49861 tcgtgcctcg ccaggacggc gcaagccggc ccggcttatg gcaagagcta ttatctttct
    49921 acagagcaaa tgctctggca cgccaggcaa tcgcccgcgc aggagtttat cgttgccacg
    49981 gagatgggca tggtataccg gctgcgcaaa gacgtgcccg agaagcggtt ttatccggta
    50041 tcgtatcaat cttcctgcga gtatatgaag atgaacacgc tcgaaaagct gctggagtcc
    50101 ctgcaatgcg accgggtgga gatcgaggtg gatcccgaga tccgcaggcc ggccagagcg
    50161 gcgatcgagc gtatgctggc gattcagtag acacgcgctt cggcgatgct gctcgacact
    50221 taccaccggc cggtgaaaga cctgcggatc agcgttaccg accgctgtaa tttccgctgc
    50281 acgtactgca tgccgctcga cgaatacgag tggatcgagc ggcgggaaat tctgagttac
    50341 gaggaaatcg cccggctggc gcgaattttc gccggcttgg gcgtcgagaa gatccgcctc
    50401 accggcgggg agcccctggt tcgcaagcat ctcgagcaac tggtggaaaa gcttgccggc
    50461 ttgcccgggg tgaaggacct gtgcctcacc accaatggcg cgctgctcgc cgaaaaggcc
    50521 gcggcgctca aggccgccgg actcaagcgg gtaaacgtga gcatcgacac tctggacgct
    50581 gagaagttca gaaaaatcac ccagcgcggg gatttgagca gggtgctcga aggattgttc
    50641 gccgcccgcg agcacgggct gcgcccgatc aagatcaatg cggtgatcga gcgcggcgtc
    50701 aatgacgatg agattgtcga tttggccgcg ttctcgcgga aaaacggatt tgccattcgc
    50761 ttcatcgaat ttatggacgt gggaaatacg aacaactggc ggtcggagcg gatggtctcc
    50821 aaaaaggaaa tcattgaaaa gatccgcgcg catttcccgc tgcgcgaggc aggccgctcc
    50881 gacggcagcg cgccggcggt ggactaccag tttctcgatg gcagtgggga tattggcgtg
    50941 atcgcctcgg taaccgagcc tttctgctcc ggttgcaccc gcgcccggct gacagcggat
    51001 ggaaagctgg tgacatgcct gttctcctcc accgggcacg acttgaagaa attgctcaga
    51061 ggcggcgcca gcgacgccga ggttcgcgat tgtatctccg cggtctggcg cggcagaaac
    51121 gaccgctact ccgacgagcg gctggaggcg atgctctccg gccaaggcta tcagcccaaa
    51181 gcgcacagaa aaatcgagat gatcagcttg ggaggctagc ggtcccggcg gtcagaggtc
    51241 agcggaatgt ccagactttt caccttgcaa gaggcccagg cgctgcttcc tcaagtggaa
    51301 cggtggctgc gcagcgccat tgaaaacaga aaaagcgcgt tccaaatgga tgagcaattg
    51361 aacgccctgc tgatgcgcat caatctccag ggcggcatat tgccggacgt gaaccgggcg
    51421 gcggaactga aaacgggcaa ggaacaatcg ctcgaccggc tgaaaaaagc gctttcggaa
    51481 atcgaagaag ccggctgcct ggtgaaagat ctggagatag gcttggtgga ctttcccacg
    51541 ttactagacg gcgccgaggt gtatctttgc tggaaacttg gagaatccca gatcggcttc
    51601 tggcatcata cgcacgaggg attcgcaggc cgcaagtcga tcgatcgcga gttccgcgaa
    51661 cgccacaaag gcagcaagtc gcaatagctc ggtttgaacc ctgctatcga gttctttact
    51721 tttagctcgc cagtcgccgg ctgacagcgc cgtactttgc ggcttcagca aagcgggatg
    51781 ttgcagaggc ggcccgccaa aaagttcaaa aagcgccttt cggcttcttc gttcgcagtg
    51841 aacgccgctc gtttttggat tagcgtctac gtcgggggct ggaagctggc ccatgccagc
    51901 aaaaaactcg cgccaacgat cagcacggca accacggcgg cggccttcca acaccactcg
    51961 atggcgcggt tgggtcccgg gacgccggcc acgtagccca gcacgaatcc ggaaatcagg
    52021 ccgcccacgt gggcggcgat atcgatcccg ggaatcaggc tgaaaaggag tccataaacg
    52081 gcccagcgcg tatacacgct tttcatagcc atgcccagca gcgtgctgcg atgacgctgc
    52141 gcaaaggcga tcatggcgcc aatcagaccg aacaaagctg ccgatgcgcc aatggaaaga
    52201 gcgcgtgacc aaaacgtact ggccgcaaaa cccgctacgc tggaagccag gtaaaagaca
    52261 aggaagcggg ccgtgccgta agcttcttcg gtctgcgcac ccagatcgta gagcgcccag
    52321 gaattcatca gaatgtgcag cagtccgcca tgcagaaacc cggcagtgac cagccgccac
    52381 cactggcctt gcgtaagaat agaaaggctc tctttggctc caaaataata cagaacctcg
    52441 ctgttgaccg cccacaggcc cccgccggac ctctgcatgc tgaggagaac cgttgccgcg
    52501 aaaagagcgc cgtttatcag cagaatgatg atagtggtga agtgggtggg cggcaccagg
    52561 cccgccagcg ccgcgcccgc agtcacgcgc tgggcatagg tgcggccgag gggttcgtgg
    52621 cactccgtgc agatgcgctc cgagctgtct acgaatgccc ggcaatgagg gcacatgcgg
    52681 agattggcca tggactctca ttgtatcagc gggccgccgc gcgccagcca ttacaaacta
    52741 acgagcaggc catgcagaag cgtgccgaaa tcgccgccgg acgccgattg gtcaggcccg
    52801 ttgccttcca tcaaatggcg ggagaaagaa caaggcggca ctgcgctttg ggcctgctcc
    52861 gccttgtttc ataaccggtg aaaatcactt aacggcgacg tcaattgaca ttactgtgct
    52921 gccgtgagcc gagcgggaaa atacaaaacg aatcttggta cgataggact ctgtagctat
    52981 ggcggcaaaa agccggggac gaaaggagtc tacgcccgcc ctgaagcgcc gggcatggct
    53041 gggccgcgcc ggcttagcag ggattgccgg ggcggccggc ctcgcttatg gagcggcgcc
    53101 gcgcttgtgg aatgaaatgg ctcgggaaac gcttcgtccc gtagagccgc cgcccgagcg
    53161 cccgcggccg tttcaatggc cttctactgg aatttgcgcc gcctggctgg ggcacagcac
    53221 cgttctgttg cgcatcggag gatgcaccat cctgaccgat ccgattttca gcgaacgtgc
    53281 gggggtgagc ctgggtccta ttacactggg aatcaagcgg ctcgtagcgc cggcgctctc
    53341 tctggagcag ctacccaaaa ttgatctgat ccttctctcg cacgcgcaca tggaccactt
    53401 cgatctcccc tcgctggcgc ggctgggaag cttgcaaacc accgtcatca ctgcgcccaa
    53461 cacgagcgat ctgcttcgcg tgaagcggta ccggcaggtg cgcgagctcc gctggggcga
    53521 gcggttccgc gtaggtccgg tagagtgccg cgccatcgaa gtgaaacact ggggcgctcg
    53581 gatgcgcagt gatacatatc gcggctacaa cggatatctg atcgaagcag cgaatcgcag
    53641 ggtcctcttc gcgggcgata cggcgcacac ctcggccttc cgggcgctgc gcgattcccg
    53701 ccccattgat ctggccatta tgccgattgg ctcatacgaa ccctggatcc gcaatcactg
    53761 cactcccgag caagcctggc aaatggggaa tgatgcgggc gccgagtact ttctgcccgt
    53821 gcatcatcag acattccggc tgagccgcga acctctgcgg gagccaattg aacgcttttt
    53881 ttccgtcgcg gcgaatcaga gctaccggat ccggctggaa aaaatcggcc aggaattctg
    53941 gctggcctag gcgcaaccct ttcggctcac agtcacagcc tgcccttact tcctggcgcc
    54001 gccaattcgc cttacagctc gataacggct ttggctaccc cttcggcgta ggcggcggcg
    54061 gtttggaacg ccttatcggc ctgagaaaga gggaagcggt gggttatcag cttcgccgcg
    54121 tcgatcttgc ctgattcaat caagcgcaag gctgtgtggt cgttccggtt ggagcgcttc
    54181 aatgcgagca gcgtggcttc gcggttcagc gccgcataca ggtcgacccg cggcatctct
    54241 tcgctgggta taccgatcag gacgactcgg ccgccgtggc gcacagcgtg cagcgcgggg
    54301 ttcagggaat ccggtttgcc cgccgcgtca atgatcaggt gcgcgccccg cccacttgtc
    54361 aaatcgagaa tagcttctgc ggccggctgc ctggcgacat cgatcgccgc gtccgcgccg
    54421 agctcctggg cgagcgcaag gcggcacggg atgcggtctg cggaaattat gcgccccgcc
    54481 ccagcgattc gggctacggc gacggccaga agcccgatcg gcccggctcc catcacggcg
    54541 accgtgtcgc ccggctcgag gcgagtcaac tccagcgcgt gaagaatcac tgccagaggc
    54601 tcgaccacag tggcgaccgg gtagctcatg ctcgacggaa tctctaccgc gtttcttgcg
    54661 ggaatgctgg cccattcacg caagaagccg ggcaactgca accctcccat gaagacgacc
    54721 ttctcgcaca agttgtgccg tcctgcccgg caaaactcgc actccccgca cgtgatcgcc
    54781 ggctcgatag ccacgcgggt ccccggcctg aggccctgaa ctcccgggcc caccgccgcg
    54841 acctctcccg ccggttcgtg cccgagaacg atgggataag tggcggcgga tcccgcgcag
    54901 cttccctccc ggtagaagtg catgtccgaa ccgcagattc ccacagcgcg cacgcgtacc
    54961 gtcacctcgc ccggccccgg ctcgcgcggc tccggcatct cacgcagctc aacgcgttcc
    55021 ctcgccacca gttgaacgga ttgcataagc agacattcta taatgtagct ggcagcgttc
    55081 caaaacatag aacggcaagc tgcaatactc cgattttctg gcgaggggcc gcgcgcaatt
    55141 ggaatggccc agcctcccat gcggggcgct tactcactgg acgacaaata cacgcgggaa
    55201 gaggggatcg tctttctcaa cggcatccag gcgctcgttc gtctgccgat ggagcaagtc
    55261 cgtcgagatc ggcgcgcggg gctgcgcacc ggcgttttca tttcgggcta cgagggatcg
    55321 ccgctgggcg gctacgacct ggcattgcag cgcgtggaac gcctgctgca cgaacacaac
    55381 atctattttg ttcccggcgt caatgaggag atcgcggcca cctcggtgat gggcagccag
    55441 attcaccaat tatttcctcg tcccaaattc gacggcgtgg tcggcatctg gtacggcaag
    55501 ggtccgggag tcgatcgatc gggcgacgcg ctccggcacg ccaatttcgc cggtacggga
    55561 aagaattgcg gcgccctggc tctggccggc gacgaccacg cctcgaagag ctccaccatc
    55621 ccgcaccaga gcgatctatc gttcttcaat tttgcgtttc cgatcctata tcctggcaat
    55681 actcaggaaa ttcttgatct cggcctttac ggcatcgcgc tgtcgcgctt ttccggcgcc
    55741 tggacagcga tgaagatggt cacgaatgtc tgcgacggcg gcgggacagt cgaagtctcg
    55801 cccgaccgca accccgttgt tttgccggaa ctcgaattcg gcggccagcc gtaccggaag
    55861 cggatggacg gcatgctgat ttcgccccgc tcattgcagc tcgagcagga gattcactac
    55921 cgcaagctgg aggcggcccg cgcttttgtc cgggccaacc ggctgaaccg catcgtcgcc
    55981 tcgaacgccg gcgactggct cggaattatc accgctggca agagctatta cgatttgtgc
    56041 accgctttgc gcgacctcgg gctcgataag gagaatctgg cccgggccgg cctccgcatc
    56101 ctgaagctcg gcatgaccta tccgctcgag ccggcgatca tcgcggagtt cgcagcggga
    56161 ctcgctgaga ttttgatcat tgaagagaaa cggagcttta tcgagctcca gcttcgggac
    56221 atgctctaca accggcgcga gcgcccggcc atttacggca aacacgatga acgaggcgag
    56281 cttttgttcc ccgtgcacgg cgagattgac cccgacctga ttgcgaaggt cgtaggccgc
    56341 aaacttctcg agcgctccga aacgcagtcc gccttggatg gcattgcaaa gcgcctctcc
    56401 cgaattgccg ggttagaaga gctgccgaga gagccgcgcg gaacgccgcc gttgggaacc
    56461 atagaagctc ggccgccgag tttctgctcg ggttgtccgc acaaccggtc gacggccgtg
    56521 ccggatggga tgtttgccgg cgggggcatc ggttgtcatg gaatggccgg acgtctccag
    56581 catattcacc gcggtttttc ttacatcacg cagatgggcg gcgagggagc tccctggatc
    56641 ggcatcgccc cgttcaccga aacgccgcat ttattccaga acgtcggcga cggaaccttt
    56701 ttccactccg ccacgctcgc ggtcaatgcc tgcgtcgccg caggcgtgaa tgtgaccttc
    56761 aaaattcttt acaacaatgc cgtcgcgatg acgggaggcc aggccgccgc cggcgcgctg
    56821 cccattccgg agctcacgcg ccaacttgag gcgcagggcc tgaagcgaat cgtagtgctc
    56881 accgatgatt tggaaaaata ccgcgatcgc acggccctgg caggttctgc ggaggtgcgg
    56941 agccgcgacg atctggaccg cgtcatggag gatctcgccg ctgtacccgg cgccacggcg
    57001 ctcatctatg accagcaatg cgcggccgaa aagcggcggc tgcggtcgcg cgggagtctg
    57061 ccgaaaccgg agcgcctgct ggtcatccat gaagaagttt gtgaaggctg cggcgattgc
    57121 gtggctaagt cgaactgcct ctcgctttat cccgtggaga ctgagtacgg gcagaagact
    57181 cgcattcacc agccctcctg caacttcgac tattcctgcc tgctagggga ctgtccatcc
    57241 ttcgtatccg tcgtcgtcga gccggggacc gggctgaaaa gacgcgtgct gccgctgctt
    57301 cccgcgatgg aaattcccga gccggccgct aaagccgcga ttggcgagaa cgaaacgtat
    57361 gaaattttga ttcctggagt gggcggcacg ggcgtggtga ctgtgaacgc cttgcttgcc
    57421 actgccgccc tgctggatgg gtttcacgcc gcgacgctcg atcagacagg cctctcgcaa
    57481 aagggcggcg cggtggtgtc gagcatagtg gtcgggagaa ggcccgtcga actcgccaac
    57541 aagagcagct acggcagcgt ccacctcctg ctcggatgcg acttgctcgc cgccggtgcg
    57601 cccgataatt tgaagcggac ggatccccgc cgcacggccg ctgtcatcaa ttcggcggaa
    57661 attcccaccg gcgaggccgt gcgcaagggg accgtgcatt tcggaggacc gctgatcgac
    57721 atcatccgcc gttcgacgca ccctggctct actgtgttcg tgaacgccac ccggcttgcc
    57781 gaagcgctgt tcgcgaatca catgccggct aacattttcc tgttgggcgt ggcttaccaa
    57841 gccggttaca ttccgctgtc tgcgcctgcg attgaaagag cgatcgagct gaacggcgtg
    57901 gccgccgcac aaaacctcaa tgctttccgg tggggccgga gatattacta cgatcctgag
    57961 gctgtcgagg aaattgcggc ccccgccgaa accagtgcag gtaagccggt tcgatcgctg
    58021 gacgatctca tcgagcggcg ctgccgcgac ctagcggctt accaggacgc actctatgcc
    58081 cagcgctatc gcgagcttgt ggaacaggtc cggcggcgcg agcaggaaat ccgcccgctc
    58141 tccaccgatc tgaccgaagc ggccgcccgc tgcctgtaca aagtgatggc gtacaaagat
    58201 gaatacgaag tcgcgcgcct gctcacgaaa ccggaatttg agcagcagac tcgggccatg
    58261 ttcgaagccc cgcgacggat tgaatacaat ctgcatccgc cgctgctgcg gtcgattgga
    58321 ctccaacgaa aattaggctt cggcccctgg ttccggcctt tgctgcgcgc cctggccgca
    58381 ctgcggatat tgcgggggac gccgctggat gttttcggcc tcagcccgct gcgccggcgc
    58441 gaacgcgccc ttattacctg gtacgggcag acgctgcgcg ccgtactcgg ggggctccac
    58501 gaatcaaatt ttgatctggc ggttcagatc gcccgcatcc cggacgcgat tcgcggctac
    58561 gagcagatca agctacgctc cattgccgcc gccgaggccc aggcggccgc cctgcttgcg
    58621 gagttcactg ctgcgccggc cgccacgcat tccagctcgt cagttcacct gtgagcggga
    58681 taagggcggc ctattgcagc ccgaaccgcg attcgatgga gttcagcgcc gcctctaccc
    58741 gtccagcttt cccatcagcg cgctttccag acgggagtat tggccctccg gcgttgtaga
    58801 ctatccttcc agcttcgtgc gctcttcgaa ccgttcagac tgcgcgaaag tcggttcgca
    58861 ggtcgccgat gccgttccta aaatctgtgc tcaggtcccc gactcgagcg ttccaatcgc
    58921 gaatccgggc gttgttgaac aaaagccgat cccagcagga ttagcgccgc gcaaagaggg
    58981 aatatacgtc tgggcctgga aatcggtcac gcttcgtttt accgacgccg catcacactg
    59041 caaaagcctg gcattcggaa tttcgagggg gcgttctaag ggcttcaccg cagctccggt
    59101 atatctgcgg ccacgcggaa tcccaaaaat tccattttct ttgcaggcgg cgtgggattg
    59161 cggaacgcgc accggagcac tttcggcagt tcatcggccc aactgctgcc tcggatgacg
    59221 cgcagcgatt tgctgtaggg ctggccgcct gctgctcccg ggtatggctc atagtagctg
    59281 ctggtccact ggtatacgtt gcccgccatg tcgtaaagaa aataagggtt tggccaaaac
    59341 tttcccactg gcgacgttcc caagtaggga tcgtgatagc cgcgcagata ctcccagttg
    59401 aaataagcgc cgtttgcctc gtcggcgaaa ttgcctgctc cctccggcgg cggccactga
    59461 tcgccccaag gaaagcggcg ggcctcgccc gtgtcgctgc cctgcgctgc gtactcccat
    59521 tcttcctcgg tgggcagccg gtacgtgcgg ccggcggcgc gttcggccgg aaggctgttc
    59581 agccagtcga tgaacgcctg ggcatcgtcg tagctcacat tgaccaccgg ctgctcatcg
    59641 ccatcaaggt tgacccctcg atacggggcg atgtgatggt tgggccggaa acggcggaat
    59701 tgcgcgttcg ttacgtcata catgcccatg aaaaacccgt gatgaaaccg gatctggtgc
    59761 aacagctcgt cacgccggtg atacggatcc gacgagagcg agccaatcat catgccctca
    59821 tgcggagggg ggatgcgcac cagacggagc ccgatcgagt tggtaaacga gtcgatccgc
    59881 tcgagcgggg tcaacaactc gggaaaatag ggccgcagcc gctcataccg aaagcggtca
    59941 tcggcggtga gttctcccag ccgcttccgg catgcaagct cagatagcaa aaggctgccg
    60001 taaacgccga aaactccgtc cttgattttg ccgttggctt cggtcagctc ggtgaaggcc
    60061 atgtgatcgt aatactcggg gccgaggtgg atctgcggca cgcgcgcggg caacttgtga
    60121 aaaaattcgc ggatgggcgc ttgcaccgga gcttcagcgt cccccgaaag atcgagacgg
    60181 tcgatgcgat ggcacagggc gcagctctgg aatacgtgaa aaccttcctt ctccggagca
    60241 attcggccct cttcatcgaa ggccaccgat tgcaacacca gcgcgccgtt ccggtcgtaa
    60301 acggaaaaat tccagaaggt gtcgcctcgt ttccttagta cctcagcctc aacaaacctt
    60361 ccctctttgt caaaggactc ggccgcgatc acggttccct cgggatagag caggcggttg
    60421 ttgttatcga aataagagat cagcgcgtcg gcgatgcggt ccttggcgat atgaagcacg
    60481 cgccgccgct tgaatgtcag gctgtcaacc ggcgccgcca gccagttggg attctcgcgg
    60541 tatggatgtt cgcgaagcag cgcagcgaga ttcggatacg ggcttgtgag ccggtaataa
    60601 tgctccgcca tgtaggcgtc catctgctcc gagcttaact tgcccggcga ttgccgtccg
    60661 gcgatctgcc gcaacagagc ggctgcattc gggggaacct gcttcaaatc tatgtaaatc
    60721 tccttgcctt ctcgccggac gatacggccg ctcatcacct tgggagatcc agcagcagtc
    60781 cccagcaggg tttgcagttg gtacagaaaa gaagcggcga ggtagtggct gttatgaacc
    60841 gtgcggccgc gcaataccac atgcagccag tccgctgaat ccggcgcggg agaggcggcg
    60901 ggtttcctgc acgcggaacc gagaagcaga aagaagagca gcgcggatgc gatcgaaact
    60961 cgcaccagcg tgattatagc gagaagccgc ccgccatgat tgctggcggg cggccactaa
    61021 aagtaaattc ttctggttgt ggcctgcggt cccaggatcg tctggttcct caggatcgcc
    61081 gccaagcctt catcgcgttg gcgccaagcc gctccccgcg ataggcgctt cagccgatgg
    61141 caggccgccg ataggccgga tcgcgagatt caaagcgtgc tggactgcgg ctgggttcgg
    61201 tctcggtgaa tccatcggta gacaacagtt gaattcttcc agaaaaattt ttctgcgacg
    61261 gcgggccctt tcgcggaaaa gtaccgctgc acgacgttta gcacttgcgg gtacgatccc
    61321 cattgatcgc tgttcggcca gtcactgccg taaaaaagcc ggttttcgcc aaaaatctcc
    61381 cagaactcgt ccagccttgg gcggtagaaa tttggatcgt cgggcacgcg gccgttcaca
    61441 tggcgaagaa cccccgacac cttgacatag acttgaggcc gcttcccgag ctcccgcaag
    61501 tgctcctggt aagccttgcc cgccgcagcg tcagtgggtg gctctagctg cgccaagtga
    61561 tcgatgatca cccgcaggtt cggaacccgg tcggtcagtc gaaccacatc ggagatcaat
    61621 ccgggagtgg gattggcggt gtccagaaca agaccggcct ctgccagagc ttttaggccg
    61681 gaaatgaatt ccggcttcga aagttcggcg cctagatttc tgccccagag gttgccgtag
    61741 cggatcccgc gaaagagggg attgcggtga aacctctcca actgctggtg gaaatccggc
    61801 ctgccgggct ccaggttccc gatcgttccg acgatgatgg tgtctttggc ggcaatgtcg
    61861 agaacccact ggttgtcttc cagccatggg ctgcattcca cctcgatggc ccccgtgacg
    61921 ccgagcggaa ccgcaatttt cctgtaccgc tccggcagcg ccggctgata gagaatcgtg
    61981 ttgtccttgg gcggccaagg cacgccctga ggacgccgcg gatcgaacag atgaatatgc
    62041 gtatcgatga tcggcatgga tgcgcctttg gcggcggcat ccgccagcac gatgcccacg
    62101 gagcctccca gaaaggttct tcgattcacg gtggtattgt atcgcgactc cggcagtgca
    62161 gatctccgga caatccgctc gctgccgata gaggcgatca actcgcggct gcggcgagga
    62221 gcaagctaag aagtaagtcc ggcccggcca ttcggaaata ccagttggtt ctgcgttgct
    62281 ctgtttttcc ttcgaatgtt acagtaccaa gcacccctgt tccctcaggg gcagcggatt
    62341 ccggtgaagg actcggaaat tagaggaaga cggagtagaa gaatatgaca ttctggccac
    62401 gaacagccct ccaactcgcg ctaggcgtcg ctgtgctgct gcaaactttg ccggcagccc
    62461 agttggctga caaaaaggcg ctgacgctcg agattgcgaa acagatcgcc gcagcggcgg
    62521 aaaaggaggc ggccggcaac aaatggacca tggtcatcgc catcttggac gacggcggca
    62581 acctgctata tctcgagcgc atggatgata cacagatcgg cagcattgaa gtcgccatcc
    62641 agaaagcaca tagcgccctg gctttcaagc ggccgacgaa ggtttttgag gacgcggtag
    62701 ccggcggccg cacggctatc ttgaagcttc caggagcaat gccggtcgaa gggggcctac
    62761 cgatagtttg ggagggtaaa gttatcggcg cgatcggatg cagtggagga acctcgcagc
    62821 aggatggatt agctgccaaa gccggcgtgg atgccctgac gaaaatcctg ggccgctagc
    62881 ttctttacgc caggccgcgg accacgacaa ccgcggcccg ggtttttctt ttcctgcgcg
    62941 tcaggcatct ccggccgcgg gccgcccgga agctcgcgcg gcgctgtttc tcagacgttc
    63001 accggctgat cgatgatttc ttccttttgg ggatcccaat acagcttctt gccgctccaa
    63061 tacgattggg ccgccataat gcagcagatg gcgtgcgaga aaccgtgatc aatattcgcg
    63121 tcgggctgct tgcggtcgcg catggcttga agccagtttt cgagatgatg cg
//
LOCUS       DASREK010000314        10203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1632192, whole genome shotgun sequence.
ACCESSION   DASREK010000314 DASREK010000000
VERSION     DASREK010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632192"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFA54_17105"
     CDS             <1..182
                     /locus_tag="VFA54_17105"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZS52590.1"
                     /translation="NPFPERPPRFVRGLYYEYRFTTPPERKRTGLWWHRNLVGLYLPP
                     ASLEAVRPDLEPASE"
     gene            468..1556
                     /locus_tag="VFA54_17110"
     CDS             468..1556
                     /locus_tag="VFA54_17110"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52591.1"
                     /translation="MPQPAELAAQDLKALAAAVPPCITILLPLPEPSEIHIRLKNAVR
                     YAEKSLRERNVRPEEIEALLQPVQALAETIEVEGRWGNALVVLRSPAVFRHFWVRRMP
                     REIVAVGEQFALHPLLSLFGSTSIFYVLALSQKHIHLFRCTEDSFERVRLPDSVPVNL
                     NVWLRTRQPDHVLDNRAFGGPSAGSMRGVMFGTSTDREDKEENLLHFFREVDKGVHNV
                     LKDGAAPLVLAGVEYEVALYRRVNTYPRLLEAAVYGSPESFGSELHARALQIVEQHSP
                     EPLRSALAQFERYRGSTRVSFDVEQTLQAAHFGRVANLFLRRDNSGSDDDKINGAAIQ
                     TLLHGGQVFVLNQEQMPDGAALAALMRY"
     gene            1584..2999
                     /locus_tag="VFA54_17115"
     CDS             1584..2999
                     /locus_tag="VFA54_17115"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HZS52592.1"
                     /translation="MTPFVFLRLLGVVYLIAFLSLYPQVAHLIGEHGILPAADYLRYL
                     RRTIGARAYYSAPTLLWLNAGGAFMKALCAAGAALSIAVTSGFTKPPLLAALWVLYLS
                     LFTAGQVFLSYQWDVLLLESGFLAIFLPLWPGAVVWLFRWLLFRLMFLSGTAKLLSGD
                     PAWRNLTALCFHYETQPLPNRLSWYMHQLPAWFQKLSTLFMFFVELAAPFLYFGPRPL
                     RLWAAGATILLQTLIQLTGNYTFFNLLSMALCVFLPMRTSFDADGGIFTAGHAVVLVL
                     AMLIAVVSTCRLWITFTGRTIPKPLSELLSALAPFPIVNNYGLFAVMTTERWEIIIEG
                     SQDGVNWREYEFKYKPGDIHRAPCFVAPHQPRLDWQMWFAALSPYYNVWFVNFIVLLL
                     QGSPSAQSFLRHSSLAGADPPKYIRALLYQYQFTDAKTKMATGAWWKRSLLGEYLPPL
                     TLSQNIYGREDLPQENPVLSA"
     gene            complement(3087..4247)
                     /locus_tag="VFA54_17120"
     CDS             complement(3087..4247)
                     /locus_tag="VFA54_17120"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HZS52593.1"
                     /translation="MNRIRVNAATGFIAGAALMYAFDPISGKRRRTVAFDKCFSAWKS
                     FLCELDKARRDAGNRTHGLLAGVEAIFSNSETDEDILIQRARSRIGRIVSHPHAVTLR
                     QENGRIVLEGPVLEEEFPQLLREVRSVFGSREIVNRLEVHRGRENIPALQGGVHRTRR
                     SELAQQSWTPVLRVAAGGTGAALLCYGLRTRNARRWAGVLAGTALLARSLSNREFRQI
                     VGIGCGPRAVEFDKAIHIHAALEEVFKFWTHYDNFARFMTHLKEVRDLGNGRSRWVAE
                     GPAGVPVSWEAEITSYIPNKLLAWRSVPGSGIETEGVVRFDRNPDGSTRVGIRLFYTP
                     PAGVLGHLVASLFGADPKREMDDDMVRLKSLIECGKTRAHGVKVTREELTAA"
     gene            complement(4737..5507)
                     /locus_tag="VFA54_17125"
     CDS             complement(4737..5507)
                     /locus_tag="VFA54_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52594.1"
                     /translation="MLDRRAFLGSLLATAAAARPTEVPSRTAKVQRLFKSPDGHPNAL
                     EASSEGLWVADQITDRAHLMSWEGKLIKTVETESSNTSGMAYGGGFLWMAANGKAMRR
                     TPRPTDASRGEVLKVDPATGKSVARYPLPDGGGVHGLEYTREGLWVTFLKSRKLALVD
                     PKDFRVIRQIPMQFDRAHGLGRDKDGIWVVFTSDRVIQKLDVKDGTVLEQISIAKEDP
                     EPHGLCLHNAHLYYCDAGLELGGKGPDGKWAGWICRIA"
     gene            5989..8046
                     /locus_tag="VFA54_17130"
     CDS             5989..8046
                     /locus_tag="VFA54_17130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52595.1"
                     /translation="MRYLLLLVLACLVSPAQTQDLASDPTPKSEPERAYLEKAYQALR
                     EKKYLDAIQQFLDAIQAAPRRAGIRKDLGYVYLKIGETESARDTFEQALSLDPQDFRV
                     ALELAFLSYETGQEARALQLLDQVRQSGDADSKKTAEEAFDRIDRALRASIEQWSMVV
                     TQDPQNRAARLELGSYLEKHREPARAAEQYLAAWRLPPRREETLLELARSWHDAGDEQ
                     GAVGAWFLASRSPETRVAERARSRLPRRHPFAGEYRRALDLDPSNTALRRDLAFLWLA
                     VQQPENAIRELETVISQDPRDMLATAQLAFLYLEKHQPERAAPLLEKVAAGPDAALAG
                     KARRALDVAAGGKKGEIAPSRLTRREQAQPHKALGEKSLALSYLKDALREFQLAHEID
                     PEDSEVALKLGIVHNILHDDREAVRWFREAMNSPDAAVAAEARRSYNNLAPQFRRIQT
                     FLWMLPMFSTRYHDVFQYAQLKTELRLGSFPVRPYLSLRLVGDWKRKTGEPLPQFLSE
                     SSLIGAIGVRTTAWNGFLLWAEAGEAASYLHTPPPGTPRLGPDYRGGLSWFRSFGATL
                     GGESAGPFAELNVDAVFLSRFQDNVISYWQMRHGYRLPPLGMLRSQLFWNTNVTFDRR
                     REYYANFTEFGPGVRFRVPLLSPPLDMTISGLRGVYLINLFNPRKPNYYDLRVGLWYS
                     VAR"
     gene            8006..9952
                     /locus_tag="VFA54_17135"
     CDS             8006..9952
                     /locus_tag="VFA54_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52596.1"
                     /translation="MTCAWGCGIRWLASLAFFSAAAAAGAQSPTGSPAANPGWARVLG
                     SVGLDLAPGPPSFVLLEGDSPQSRQWGFRPTEKSVRVASVEELREPKLEVVWQKPVTA
                     PVYELPPGARVFTREKWTGAPLVAGVRRDGKAVLWTITDIGERGYERFPYLLHALNSL
                     GVEFPFRGGRTWAFFDDSYRLRADSDFLARRWRKAGIAAIHAAAWHFYEPDAGRDEYL
                     RKLIEACHRQGILVYAWLELPHVSEQFWEDHPQWREKTATQQDAHLDWRKLMNLADPN
                     CSQAAEAGLRALLNRFDWDGVNLAELYFESLYGPSAPDRFTPMNDVVRADFRAKAGFD
                     PIELFRQDSPRYWERDRAAWKRFADYRASLTVRMQQRWLQAARGWKPDLDLVLTHIDD
                     RFDAAMRDNLGADAARLLPFLDRLNFTFAVEDPATVWDLGPERYAEIARRYARLTRRL
                     DKLAVDINIVPRYQDVYPTKKQVGSELFQLLHGGSQSFARVLLYFEQSIGAADYDLAA
                     AASAAVSRVEIEGRARIITAAQTTGLRWQGPARVDGKPWPGVDGSIAWIPAGRRRIER
                     DSAPLRFHLLWLNADLIGARALPEGVELQYRSSARAPALVDFRPGEIEIDGRPAKAAV
                     IEAEDHFVVLLPPGERLARISAAR"
     gene            10037..>10203
                     /locus_tag="VFA54_17140"
     CDS             10037..>10203
                     /locus_tag="VFA54_17140"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZS52597.1"
                     /translation="MPESKAPQIPDRLYFRIGDVSELLGVEPYVLRFWETEFPQLQPK
                     KGKTGHREYKR"
BASE COUNT         1882 a         3158 c         3054 g         2109 t
ORIGIN      
        1 aaaatccgtt tccggagcgg ccgccgcgct tcgtgcgcgg gctctattac gaatatcgct
       61 tcactacgcc gccagagcgc aagaggacag gcctttggtg gcatcggaat ctcgttgggc
      121 tgtacctgcc gccagcaagc ctcgaagccg tgcgccccga cttggagccg gcatcggaat
      181 agtgcgctgc ccgccggctg gcgctgtttc tgctcggctt cgaggccgga gcccatcaaa
      241 aaagccggcc acccacgctt catcggttgc gcaggagatt tacgtgtgtt gttctcctac
      301 aactgtacgg cgccgcacaa aagttcggat tcgtcacgtg gcctgctacc gccgcttcgt
      361 gagcggcatc ccattaattt ggaggagcga tgggtatgat ggatcaagga gttcccgtca
      421 cgccaaagaa gccatcggtt gtaaatccaa atatctggag tcaggagatg ccgcaacctg
      481 ctgaactcgc tgctcaggac ttgaaggcat tggccgctgc tgttccgcct tgcatcacca
      541 ttctgttgcc gcttccggag ccttcagaaa tccacatccg gctgaagaac gccgtccggt
      601 acgcagaaaa gagcttgaga gagcgaaacg tgcgtccgga agagatcgag gcgctgttgc
      661 aaccggtgca ggcgctggcg gagacgatcg aggtagaagg ccgttgggga aacgccctgg
      721 tggtgctgcg ctcaccggcg gtgttccgcc atttctgggt gcgcaggatg cctcgggaga
      781 tagtggcggt gggagagcag tttgctctcc atccgcttct gtcgttgttc ggcagcacca
      841 gcatcttcta tgtgctggcg ttgagccaga agcacattca tctgttccgc tgcaccgagg
      901 acagcttcga gcgggtcagg ctgccggact ccgtccccgt caacctgaat gtatggctgc
      961 gtacccggca gcccgaccat gttctcgaca accgggcttt cggcgggcct tctgcgggat
     1021 cgatgagagg tgtgatgttc ggaacaagta cagaccgcga ggataaagaa gagaacctgc
     1081 tccatttttt ccgcgaggtg gacaagggag ttcacaacgt gttgaaagat ggcgctgctc
     1141 ccctggtttt ggctggggtg gaatacgaag tggccctgta ccgcagagtg aacacctatc
     1201 cacggcttct ggaagccgcc gtctatgggt cgcccgagag tttcggcagc gagctgcacg
     1261 cccgcgcgct ccagatcgtc gagcagcact ccccggagcc gctccgaagc gcactggccc
     1321 agttcgaacg gtaccgcggt tcgacccggg tttccttcga tgttgaacag acattgcagg
     1381 cggcgcattt cggccgcgtg gccaatcttt ttcttcggcg ggacaattcc ggcagcgatg
     1441 acgacaagat caatggggcc gccatccaga ctctccttca cggaggacag gtgttcgttt
     1501 taaatcagga acagatgccg gacggcgcag cgctggcggc gcttatgcgc tattgagcgt
     1561 gccggcgcac gtccaaacca gccgtgacac cgttcgtttt tctgcggctg cttggcgtcg
     1621 tttacctgat cgcatttctc tcgttatacc cgcaagtcgc tcatctgatc ggcgagcatg
     1681 gcattctgcc ggccgccgat tatctccggt acctgcgccg gaccatcggc gccagagcct
     1741 actattccgc ccccacctta ctctggctca atgccggcgg tgcgttcatg aaagcgctgt
     1801 gcgccgccgg agcagcgctt tcaatagcag tcacgtcggg cttcacgaag ccgcctctcc
     1861 tggcggcgct ctgggtgctg tacctatcct tgttcaccgc cggtcaggtt tttctctcgt
     1921 atcaatggga tgtcctgctg ttggaaagcg gttttttagc gatcttcctc ccgctgtggc
     1981 cgggagcggt cgtgtggctg tttcgatggc tgctgttccg actgatgttc ctgtcgggca
     2041 cggccaagct gctcagcggc gatcccgcct ggagaaacct cactgcgctc tgcttccact
     2101 acgaaaccca gccgctgccc aatcgcttgt cgtggtacat gcaccagttg ccggcgtggt
     2161 tccaaaagct ttcgactcta ttcatgtttt tcgtggagct ggcggcgccc tttctgtatt
     2221 tcggcccgcg gcccctccgg ctgtgggccg ccggcgccac cattttgctc cagaccttga
     2281 tccagttgac cggcaattac acttttttca atctgctcag catggccttg tgcgtgttcc
     2341 ttccgatgcg aaccagtttc gacgcagacg gggggatttt cactgccggg cacgccgtgg
     2401 ttctggtcct cgcgatgctg atcgccgtgg tcagcacctg ccgcctgtgg atcactttta
     2461 ccgggcggac catccccaaa ccactatcgg aactgttgag cgcgcttgct ccattcccca
     2521 tcgtgaacaa ctatggcttg ttcgcggtaa tgactaccga gcggtgggaa atcatcatcg
     2581 aagggagcca ggatggcgtg aactggcggg aatacgaatt caaatataag ccaggagaca
     2641 ttcaccgggc gccctgcttc gtggctcctc atcagccgcg cctggattgg caaatgtggt
     2701 ttgccgcgct aagcccgtat tacaacgtct ggttcgtgaa tttcatcgtg ctgcttctcc
     2761 agggttcgcc gtctgcgcag tcatttcttc ggcacagctc tctggccggt gccgatccgc
     2821 ccaaatacat ccgcgcgctg ctttatcaat accagttcac tgacgctaag acgaaaatgg
     2881 ccaccggcgc atggtggaag cgctccttgc ttggcgagta tttgccgcct ttaacgcttt
     2941 cccaaaatat ttacggcaga gaggatctgc cccaggaaaa tccggttcta agcgcgtagc
     3001 ttccagaagc ggcggtccgg gtcgaggcgg ccgccaggta caacgccgct cgtccagatc
     3061 gccgccccgg ccccggttcg ggcagctcac gccgcggtca gctcttcgcg ggtgactttg
     3121 accccgtgcg cgcgggtctt cccacattcg atgagcgatt tgagtctcac catatcgtca
     3181 tccatctcgc gcttgggatc ggcgccaaag agcgatgcca ccaaatgccc cagcacgccg
     3241 gccggcggcg tatagaaaag ccggatgccc acccgcgtgc tgccgtcggg attcctgtcg
     3301 aaccgcacga cgccctccgt ctcgatcccg gacccaggca cgcttcgcca cgcgagcagc
     3361 ttgttaggaa tgtaacttgt aatttccgct tcccacgaaa ccggcacgcc ggccggacct
     3421 tccgcgaccc agcgcgagcg tccgttcccc agatcgcgaa cctccttcag gtgcgtcatg
     3481 aagcgcgcga agttgtcgta atgcgtccag aatttgaaga cttcttcgag tgcagcatgg
     3541 atgtggatcg ccttgtcaaa ctccacggcg cggggcccgc agccgattcc cacgatttgc
     3601 cggaattccc tgttagagag ggaccgggcg agtaaggccg tcccagccag aacccccgcc
     3661 caccgccggg cgtttcgcgt ccgaagcccg tagcagagca aagcggctcc ggttccgccg
     3721 gcggcgaccc gcagaaccgg agtccaactc tgctgcgcca gttccgagcg cctcgttctg
     3781 tgaactccgc cctgtaaagc aggtatattt tcccggccgc ggtgcacctc cagccggttg
     3841 acaatctctc gagagccgaa aacggacctc acttcccgaa gcaactgcgg aaactcctcc
     3901 tcgaggaccg ggccctccag gacaatgcgg ccgttttcct gcctcagggt cacggcatgc
     3961 ggatggctta cgatccttcc aattctggat ctggcccgtt ggatgaggat gtcttcatcc
     4021 gtttcggaat tggaaaaaat cgcttccaca cctgccagca gtccgtgggt ccggtttccg
     4081 gcgtctcgcc gcgctttatc cagctcgcaa agaaaactct tccatgcgct gaagcattta
     4141 tcaaaagcca ccgtccggcg cctttttccc gagatcggat cgaaggcgta catgagagcg
     4201 gcgccggcga taaacccggt tgcggcattt acgcgtatgc ggttcatggt ccctccagtt
     4261 ctgttcctta catttttgta ctccaatcgc cagagcttac accggaaaca aaacaagctt
     4321 gcaatcgccg gtccggtcag ctccggcaga ctcgtcctct tccccaaaaa ccgtaaggga
     4381 ggcggtttca cagtctatcg ccaccgtatt tgttgttctg actgacgtga cagcgcagga
     4441 cgagagttac atggaggcag gacttcctag tgcggtcggc ggaagtttga gacgccgccc
     4501 gcctgattat cggccgtcaa ttgctcgccg ttacaacctt tgagattcgc gttggggcta
     4561 cttcccctct tcccggtacc gcgctgtaag cttccccgcg acggaaaggg gcaagccctt
     4621 gcgttcagct caaatagccc ggcagcaagt ctgagtgcga agattttccc agcgtcattc
     4681 agcgcgtttc cgctggcgct atagaggctt ccagaacgcg ccgcgcaagg aaagcctcag
     4741 gcgatccggc agatccagcc ggcccacttg ccgtccggac ccttgccgcc gagctccagg
     4801 ccagcgtcgc agtaataaag atgggcattg tggaggcaca ggccgtgagg ctcgggatct
     4861 tctttggcaa tcgagatctg ctcgagaacc gtgccgtcct ttacatcgag cttctgtatt
     4921 acacgatcgc ttgtgaagac gacccagatc ccatccttgt cgcggcccag gccgtgcgcc
     4981 cggtcaaact gcatcgggat ctggcggatg acacggaaat ccttgggatc gaccaaggcc
     5041 agtttgcgcg atttgagaaa tgtgacccac aggccctcgc gggtgtattc gaggccgtgg
     5101 acgccgccgc catccggcaa agggtatcgt gctacggatt tgccagtagc cgggtcgact
     5161 ttcaatactt ctccccggga ggcatcagtc ggcctaggcg tccggcgcat cgcctttccg
     5221 ttggctgcca tccatagaaa accgcccccg taagccattc cactggtgtt cgatgactcg
     5281 gtttccacgg ttttgatcag cttgccctcc cagctcatca ggtgagcgcg atcggtgatc
     5341 tgatcagcga cccacaagcc ttcggaactg gcttcgagcg cattgggatg gccatcgggc
     5401 gacttgaaaa gtcgctgcac cttcgccgtg cggctcggaa cttctgtcgg ccgggcggct
     5461 gcggccgtgg ccagaaggga acccaaaaaa gccctgcggt ccagcatcgt aacacctccg
     5521 tattgtgaaa aacgggcgcg aatgatcagc ccagcttgtg ccttcgcggc cccgccagcg
     5581 actcaatatt actcccgaga ggttgaattg tcactttgcc ccagggctgc gccgagagca
     5641 gctagagagt agagagggcg ccggcgaccg tgggtagttg tcaaggctgt ggcgaaggac
     5701 cgggccacag ctctttatac aggcacgacg gcggccagcg ggttcacgag atgttccata
     5761 tattcgacct attttctgca atctgccgga ttatgagcag gatatttcgc tgcgcctctt
     5821 gcgcctgaac ttgaaatatg ttgtgattta cacgctgcgg aaaagcgctg ggcccgccgg
     5881 cgccagccgc ggctcgggac agcgctggac cggaaggctg tttctgcctt gcgcgccgca
     5941 aagcggcagc cggtttcgca gcgcgctctt gtacgtcagg cgccgacagt gagatatctt
     6001 ttgctgctgg tgcttgcgtg tttggtgtca cccgcgcaaa cacaggatct tgcctccgat
     6061 ccaaccccaa aatcggaacc ggaaagagcc tatctcgaaa aagcctacca ggcgcttcga
     6121 gagaagaaat atctggatgc catccagcag tttctggatg ccatccaggc agcgccacgc
     6181 cgggccggga ttcgcaagga tctcggctac gtttatttaa aaatcggaga gaccgaatcg
     6241 gcccgcgaca cctttgagca ggccctctcg ctcgatccgc aggactttcg ggtcgcgctc
     6301 gagctcgcct ttctttccta tgagacaggc caggaagctc gggccctgca attactggat
     6361 caagtgcgcc aatcgggcga tgcggattcg aaaaagaccg cggaagaagc ctttgatcga
     6421 attgatcgcg cgctgcgtgc gtccatcgag cagtggtcca tggtcgtaac gcaggatccg
     6481 cagaaccggg cggcacggct cgagctgggc tcctatttgg aaaagcatcg ggaaccagcc
     6541 cgggctgcgg agcagtattt agcggcctgg cggctgcctc cgaggcgaga agagacgctg
     6601 ctcgagctgg cgcgatcctg gcacgacgca ggcgatgaac agggcgctgt cggggcttgg
     6661 tttctcgcgt cacgctcccc ggagacgcgc gtggcagaac gcgcccgcag ccgccttccc
     6721 cgccgccatc cctttgccgg cgaataccgc cgcgctctgg atctcgatcc gtcaaatacc
     6781 gcattgcggc gggatctcgc gtttctgtgg ctcgccgtcc agcagcccga aaatgctatt
     6841 cgggaattgg agaccgtgat cagccaggat cctcgggaca tgcttgccac agcgcaattg
     6901 gcgttcctct atctcgagaa gcaccagccg gagcgcgccg cgccactgct ggagaaggta
     6961 gctgccggcc ccgatgccgc gctcgccggc aaagcccgcc gggcgctcga tgtggctgcc
     7021 ggcggcaaaa agggggaaat cgcgccctct cgccttacgc ggcgtgagca ggctcagccg
     7081 cacaaagctc tgggcgagaa aagcctcgcc ttaagctacc tgaaagacgc gctgcgagag
     7141 tttcagctcg cccatgagat cgatcctgag gattccgaag tggcgctgaa gctcggaatc
     7201 gtccacaaca tcctgcatga cgaccgcgag gcggtgcgat ggttccggga ggctatgaac
     7261 agcccggacg ccgcggtggc cgctgaagcc cggcgcagtt acaacaattt ggcgccgcaa
     7321 ttccggcgga ttcagacttt cctctggatg cttcccatgt tttccactcg gtaccacgac
     7381 gtgttccaat acgcgcaact gaaaacggag ctgcggctgg gatcatttcc cgttcggccg
     7441 tatctgtcgc ttcggctggt cggcgattgg aagcgcaaaa ccggagaacc gttgccccag
     7501 ttcctttccg aaagctcctt gatcggcgcc ataggggtgc gcacaacggc atggaacggc
     7561 tttttacttt gggctgaagc gggagaggca gcgagttatc tgcacacgcc gccgcccgga
     7621 acgccccggc tggggcccga ttatcgcggc ggcctgtcgt ggttccgcag ttttggcgcg
     7681 acgctcggcg gcgagtccgc gggcccgttc gccgaactca atgtcgatgc cgtttttctc
     7741 agccggtttc aagacaacgt gatcagctac tggcagatgc gccacggcta tcggttgccg
     7801 ccgcttggaa tgttgcggtc gcagctattc tggaatacca acgtcacttt cgaccgccgg
     7861 cgcgagtatt atgccaactt cactgaattc ggcccgggcg tgcgattccg tgttcctctt
     7921 ctctcccctc cgctcgacat gaccatcagc gggctgcgcg gagtctacct gatcaacctg
     7981 ttcaacccga gaaaacccaa ttactatgac ctgcgcgtgg ggctgtggta ttcggtggct
     8041 cgctagtctc gcgttcttct ccgcagcagc cgcggccggc gcgcagtccc ccacgggcag
     8101 cccggcggca aacccgggtt gggcgcgggt tctagggtcg gtgggattgg acctggcgcc
     8161 cggaccgcct tcgttcgtac tcctggaagg cgattctcct caatcccggc agtggggctt
     8221 tcgtccgacg gaaaaaagcg tccgggtcgc cagcgtcgag gaattgcgcg agccgaagct
     8281 cgaggttgtg tggcagaaac cggtgacggc gcccgtatac gagctcccgc cgggagcccg
     8341 cgtcttcacg cgggagaagt ggaccggcgc gccgttagtg gccggcgtgc ggcgtgacgg
     8401 caaagctgtc ttgtggacga tcaccgatat tggcgagagg ggttatgagc gtttccccta
     8461 ccttctccat gccctgaatt ctctcggcgt cgagtttcct tttcgaggcg ggcgcacctg
     8521 ggcgttcttc gatgattcgt atcgcctgcg cgccgattcc gacttcctcg cccggcgctg
     8581 gcgcaaagcc ggcattgcgg cgattcacgc cgccgcctgg catttctacg agcccgacgc
     8641 agggcgagat gaatacttac gaaaactgat agaagcctgc caccgccagg gcatcctggt
     8701 ttacgcctgg ctcgagctgc cacacgtcag cgaacaattc tgggaagacc atccgcaatg
     8761 gcgggagaaa acggcgacgc agcaagacgc gcatctggac tggcgcaagc tcatgaacct
     8821 ggcagatccc aattgctccc aggccgccga agcggggctg cgcgcgctgc tcaaccggtt
     8881 cgattgggat ggagtcaatc tggccgagct ttatttcgaa tcgctttacg ggccttcggc
     8941 tccggaccgc tttactccta tgaacgatgt agtccgggcc gattttcgcg ccaaagcggg
     9001 tttcgatccg atcgaattgt ttcgccaaga ctcgccccgc tattgggagc gggaccgagc
     9061 agcgtggaaa cggtttgccg attatcgggc ctccctgacg gtccggatgc aacagcgctg
     9121 gctgcaagcc gcacgcggct ggaaacccga cctcgacctg gtgctcaccc acatcgacga
     9181 ccgcttcgac gctgccatgc gggacaacct gggggcggac gcagcccggc tgctgccctt
     9241 cctcgaccgc ctgaatttta cgtttgcggt cgaggatccg gctactgttt gggatctcgg
     9301 gccggagcgt tacgccgaga tcgcccggcg ttacgcccgg ctgacgcggc ggctcgacaa
     9361 actggcggtg gacatcaaca tcgttccccg atatcaggac gtgtatccga caaagaaaca
     9421 agtaggttcg gaattgtttc agttattgca cggaggcagc cagtccttcg cccgcgtcct
     9481 gctgtatttc gagcagtcga tcggcgcagc cgactatgac ttggcggctg ccgcgtcggc
     9541 cgccgtcagc cgggtcgaga ttgagggccg ggcgcggatc attaccgcgg cgcaaaccac
     9601 aggcctgcgc tggcaaggac cggcgcgcgt ggatgggaaa ccgtggcccg gcgtggacgg
     9661 gtcgatcgcg tggattcctg ccggccggcg ccgcatcgag cgcgactcgg cgccgctgcg
     9721 cttccacctc ctttggctga acgccgatct gatcggcgcg cgggcgctgc cggaaggcgt
     9781 agagttgcag taccgctctt cggcgcgcgc gccggcgctg gtcgatttcc ggccgggaga
     9841 gatagagatc gatggccgcc ctgcgaaggc ggcggtaatc gaggcagagg atcatttcgt
     9901 ggtgctgctg ccgccgggcg agcggctggc gcgcatttct gccgcccgtt gatcgtggca
     9961 gcaacggcag gtggcgtttt cgcccttccg gccactgcat cctagcagcc ggcagagatg
    10021 ctaccattgt tttcgaatgc cggagtcgaa agcgccgcaa atccctgacc ggctctattt
    10081 ccgaatcggc gacgtcagtg aactgcttgg agtggaaccg tacgtgctcc gcttctggga
    10141 aacggagttt ccccagcttc agccgaagaa aggaaaaacc ggccaccgcg aatacaagcg
    10201 aaa
//
LOCUS       DASREK010000315        10118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2133343, whole genome shotgun sequence.
ACCESSION   DASREK010000315 DASREK010000000
VERSION     DASREK010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133343"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFA54_17145"
     CDS             complement(<1..327)
                     /locus_tag="VFA54_17145"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS52598.1"
                     /translation="MFLKILAHPIFTSLHFHMPMIVDLSHPLVMLQGENGSGKSTLLH
                     SLFYALRGEQIDGYVYKLECGNVQLGKVFLFDAEQHNPRTQLDFFKNQPEMLEFLQMA
                     SHGQVML"
     gene            complement(486..1601)
                     /locus_tag="VFA54_17150"
     CDS             complement(486..1601)
                     /locus_tag="VFA54_17150"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HZS52599.1"
                     /translation="MLSVFCCPSRYTQGKNATAFLGREMKTVGLEGPALIVAGRSAAK
                     LLSQTWCTSLGEAGIVHHVHPFGGECSAAEIERVKASAREVRARVILGAGGGKVLDTA
                     RAAAADLQLPVVNCPTVASSDAPCSALSVIYTDEGVFQEYRIYGKNPDLVLVDTQVVA
                     QSPLRLLVAGMGDALATWFEAKTCVDGNVKNMRGGASSQSALALAELCYRTLLADGPA
                     ACRALQANAVTPALERLVEANTLLSGLGFESSGLAAAHAVHNGLTAAPGTHPFFHGEK
                     VAFGTLVQLVLEGKSRAQFEEVLSFATEVGLPVTLAEIGLHELPKDLLHQIALRATAK
                     GETIHNEPFEVTPEMVADAIRAADATGRAWKGSLAKQ"
     gene            complement(1749..2684)
                     /locus_tag="VFA54_17155"
     CDS             complement(1749..2684)
                     /locus_tag="VFA54_17155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZS52600.1"
                     /translation="MKTGVIVNPHSAGGKTGRLWPAISGCLARHLGPVEIRFTEAPGH
                     ASSLARELIERGCQRIIAAGGDGTISEVASGFLKDDQPLNPDACLGIVPLGTGGDFIR
                     SLKIPRGPGDAIQAIADGAALRIDVGKARFQGNDGTPQSRYFVNLLSFGMGGEVATRS
                     RNWLNPLGGRVSFLYATAAAFLRYDLKTVRLRLDGNADALSFSVYNVAVGNGRFHGGG
                     MQACPAAVLNDGLLDITIIENMSRFQAIRDFRILYSDNVYRHPKVKHLRARELHAEAE
                     EPVRIEIDGEPLGTLPLEVTVLPQRLPVLVPAGLI"
     gene            complement(2823..3815)
                     /locus_tag="VFA54_17160"
     CDS             complement(2823..3815)
                     /locus_tag="VFA54_17160"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZS52601.1"
                     /translation="MKPVLVTGATGFLGKHLVEQLRRVDASIRLRVFCRGPSPWDGDP
                     AVEVIRGDITSPEDVDRAVAGAGQVYHLAGIVSRNPRDVRELYRLHIEGARNLCRAAL
                     RRGTEKIVLVSSSGTIAVSREPVIHDEQSGYKNDIVAEWPYYLAKIFEEKLALSWVRL
                     YGLPIVVVNPSLLLGPGDDRNSSTGDVRLFLEGQILAIPLGGLNFVDVRDAAAGLIAA
                     MRAGRPGERYLLAGENWTFREMITAVSEIAGVRAPKRQLSLRASLRGAAVLRRLYPLL
                     GKKYGLDDASIKMSALFWYCTPEKARRELGFQTRDPKETLRETVEDIRRRSGHG"
     gene            complement(3979..5652)
                     /locus_tag="VFA54_17165"
     CDS             complement(3979..5652)
                     /locus_tag="VFA54_17165"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HZS52602.1"
                     /translation="MRSASIPAPLDIDPAPNAVGRRALVPDAGCVVHLEADAAPRLLW
                     YGEDLLDVKMPAGTRVVYPKPSIPGLRDREGAVRYALAHPEQMEPLAALLRPGMRVTI
                     GMDDISLPLPKMPRPDIRESVLTILLELLAEKGVDDVHIIVATSFHRRMTAQEIERAV
                     GSRVFRAYYPERLYNHDGEAPGGMVELGVTDRGERVRLNRRAAESDLLIYVNINLVSM
                     DGGHKSVGVGLCDYPTLQAHHTPQTILDCDSYFDHTRSALARSCNRIGEVVERNVKVF
                     HIETVLNNAMFDPKMPFFIKNEDRYNALDRASFRLAQAGLRALPRPAKRKILFAVPAA
                     YEMIAVHAGAADPTHDKSLAYCYAQYCVPLQGQSDVVVFGIPFISPYNVNSILNPLLV
                     QVLALGYFFNMYRHMPVVKKNGVLILTHPLYDEFDPLHHPSYIEFFHRILPETRDSLV
                     LQQKYEEEFARNPDYIRMYRSGNAYHGVHPFYMWYWGENGRAHVGKVIAVGAENPDVA
                     RILGWETAASMEEALDMAQSHLGRRPSVTLMHIPPIGMADVMGSPEIVG"
     gene            5734..6252
                     /locus_tag="VFA54_17170"
     CDS             5734..6252
                     /locus_tag="VFA54_17170"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HZS52603.1"
                     /translation="MKKAPILLGMAAALWVGCGGGPSPRPEARTNDPAEIKALEDRFM
                     TAFNAKDVNAIMSCYVPDESLLVFDVIPPRQYTGANAYRKDWEGLFSAFPGPVTAEIS
                     DLDVTAGDTVAYGHSIQHVVLTGKDGKKLDMTARVTDGYKKIDGKWLISHEHVSVPVD
                     LMTAKPDLSSKP"
     gene            complement(6261..6962)
                     /locus_tag="VFA54_17175"
     CDS             complement(6261..6962)
                     /locus_tag="VFA54_17175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HZS52604.1"
                     /translation="MKIRAAFYDLDGTLIAGNVVTRYVYYVRNHPSRLRAVLEYAKLL
                     LSAPFLIGLDLYSRRLFNEVFYRQYRGLPKEWLESQAESLFEKVIRPSIYPGARDLVD
                     ADRAKGYRPVLVTGALDFDLGPVLRYFGFQDVICNSLVYDDGRATGRIAAPLIAEAEK
                     QAALIRLCHRYDIDIALSKAYSDSLSDLPMLESVGLPHAVNPDSRLRRVAAARGWPIL
                     DLKRTASGAAVARSR"
     gene            complement(6976..7566)
                     /locus_tag="VFA54_17180"
     CDS             complement(6976..7566)
                     /locus_tag="VFA54_17180"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HZS52605.1"
                     /translation="MPPMEPAFIARLAICFFAGSIPFAVLAMLGSGIDIRRVGSGNPG
                     FNNVLRVNKWRAVLTLAGDLGKGFLAVWFCLRPGDGLLAGWLYGFAAVLGHCYSPFLK
                     FNGGKGVATSAGVMLVLYPRWAAIALLLFTIVRIAGSRRKWKEAGAIGSLSAWVAFTL
                     SMLVFVGSRDALIAAVMTIFLAWRHRKNFQNLLAPA"
     gene            complement(7578..>10118)
                     /locus_tag="VFA54_17185"
     CDS             complement(7578..>10118)
                     /locus_tag="VFA54_17185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZS52606.1"
                     /translation="IVRSGASAPVDGASGAKPRYRIAPPAREAPPAPETAVTLPEVLR
                     RHAEAEPDRVHIHLPEEESVQDITYRELLAAAAEVAAGLAAEGLERDQTVAIMLPTCA
                     DFFYAFFGVSLAGGIPVPIYPPARPDKIEEYVRRQTRILNNAEVRFLITFDRVKAVSQ
                     IMRLNLPGLAAVTSVEALRQAGRGRPAPAVSPSDTGFIQYTSGSTGDPKGVVLSHANI
                     LANIRGIGYSVQVRPTDIVVTWLPLYHDMGLIGSWLFSLYYATPITVLSPLAFLSRPE
                     RWLWAMHDARGTLCPAPNFSYELCARKIPDRMLEGLDLSAWRVAINAGEAVLPDTLAR
                     FTERFSSYGFRPESWVPCYGLAESSVALAFPPIGRRPVIDAIRRKPFEELGVAEPAHP
                     HESDVIRFVANGRPMPGHEIRIVDDENRDLPERVRGRVLFRGPSKTSGYYRNPAATAA
                     VTVDGTWIDSGDLGYWGGGEVYITGRRKDCIIKSGRNIIPQEVEGAASEVAGVRRGCV
                     AAFGVTDADTGTERLVVVAETRASRSEELRRIEADILKAVEAVLGIPPDRVQLVPPQS
                     IPKTSSGKIRRFETKALFLSGELKARKPPAWVQIVRLALENAGSGSGLAARRAAAWLR
                     QAYSYGLLLGIAAAAGLAARILRLGPWTGSLLSAGARTFLLLSRYEIEVQSAALDRTR
                     PAVFVANHPGRLDPIVLAAALPPPLLFAGGDALFGISRVAAALLRPFVLASGRSAAMP
                     PGSWRRRMVEALQRGQSVLVFPDVSIGAPPHRSRFRLDPFHAAVEASAPVHPITLLGT
                     SHMLAGHPALRRKAKIVIGDAICPADKDRREMVRLREEAREAIARNWQ"
BASE COUNT         2062 a         3119 c         3182 g         1755 t
ORIGIN      
        1 caacatcacc tgtccgtggc tcgccatctg caagaactcg agcatctcgg gttgattctt
       61 gaaaaagtcg agctgcgtgc gggggttgtg ttgctcggcg tcgaagagga atactttgcc
      121 taactggacg ttgccgcatt cgagcttgta cacgtagccg tcgatctgct cgccccgaag
      181 cgcataaaaa agcgagtgga ggagagtgga tttcccggag ccgttctccc cctggagcat
      241 gaccaggggg tgggaaagat ccacaatcat cggcatatga aaatgcaggc tggtgaatat
      301 cgggtgagcg aggatcttga gaaacatagg gtcaggatag cagatggagg cggggccgga
      361 acgtgcatcg ccaactccgg caaggcaagg actgctgcga agaaggatcg cgaggctcgc
      421 agaggagcaa gccactggga gcccgccggt ggctgggttc tcggaaaatt tggcgcttgg
      481 ccgatctatt gtttggccaa acttcctttc cacgccctgc ccgtcgcgtc ggcagcgcgg
      541 atggcgtcgg cgaccatttc gggggtcacc tcaaagggtt cgttgtggat cgtctctccc
      601 ttcgcggttg cgcggagggc gatctggtgc agcagatcct tagggagctc gtgtaggccg
      661 atctcagcca gcgtgacggg aagcccgact tccgtggcaa aagacaaaac ctcctcgaac
      721 tgagcccggg atttgccttc gagaacgagc tggaccagcg tgccaaaggc gaccttctcg
      781 ccatggaaga acggatgcgt gccgggcgct gccgtcagcc cgttatgaac ggcgtgtgcc
      841 gcggcaaggc ctgaagattc gaacccgagt ccggaaagca gggtattggc ttcgaccaga
      901 cgctccagag ccggcgtcac ggcgttcgcc tgaagcgccc ggcaagcggc ggggccgtcg
      961 gcgagcagcg tccgatagca cagctcggcc agggccaggg cgctctggct ggacgccccg
     1021 ccgcgcatgt tcttgacgtt cccatccacg cacgtcttgg cctcgaacca agtcgccagc
     1081 gcatcgccca tcccggccac gagcaggcgg agcgggcttt gagcgaccac ttgggtatcc
     1141 acaagcacta aatcggggtt tttgccgtaa atgcggtatt cctggaaaac gccctcgtcg
     1201 gtgtagatga ccgagagcgc gctgcacggc gcgtcgctgg aggcgacagt cggacaattg
     1261 accacgggaa gctgaaggtc cgcggccgcc gcccgggccg tatcgaggac cttcccgccc
     1321 cccgccccca gaatgacccg cgcccgaacc tctcgcgccg aggccttaac ccgctcgatc
     1381 tcggcagccg agcactcgcc cccgaacggg tggacgtggt gaacgattcc ggcttcgcca
     1441 agactggtgc accaggtttg cgacagcaac tttgcagcgc ttctgccggc tacgatcaaa
     1501 gccgggcctt cgaggccgac ggttttcatc tctcgaccga gaaaggccgt ggcatttttt
     1561 ccctgagtat agcggctggg acagcaaaac acagaaagca tgctgcacct ccataaccgc
     1621 cgtttccggc tggttgcaga acccaaacat ttatgccgtt gtcatcgaga ggcgcataag
     1681 agagcctcag ccgcggcggc ccgcgtatcc tgaggaggcg atgccccggc ccggctggag
     1741 tccctattct agatcaagcc ggcaggaacc agaacaggca gccgctgggg gagcaccgtg
     1801 acctccaacg gcaatgttcc gagcggctcg ccgtcgatct cgatgcgcac cggctcctcg
     1861 gcttccgcgt gcagttccct cgcgcgcaag tgtttcactt tgggatggcg ataaacatta
     1921 tcggagtaga ggatgcggaa atcccggata gcctggaagc ggctcatatt ctcgatgatg
     1981 gtaatatcga gcaagccgtc gttaagaacc gcagcaggac aggcttgcat gccgccgccg
     2041 tggaaacgcc cgtttcctac cgcgacgttg taaacggaaa acgacaatgc atcagcgttg
     2101 ccgtcgaggc gcaaccggac ggtcttcagg tcgtaccgga ggaacgccgc cgccgtggca
     2161 tacagaaatg agacccggcc cccgagcggg ttcagccaat tgcgggagcg ggtggcgact
     2221 tcgcctccca tgccgaagct gagcaggttc acaaaataac ggctctgcgg tgtgccgtca
     2281 tttccttgaa agcgcgcctt gccgacatcg atgcgcaggg cggcgccgtc tgcgatggct
     2341 tgaatggcgt ctcccggccc gcggggaatc ttcaggctgc ggatgaaatc accgccggtg
     2401 cccaggggca cgatgcccag acaagcgtct ggattgaggg gttgatcgtc tttcagaaaa
     2461 ccgcttgcca cttcgctgat cgtgccgtcg ccgccggcgg cgataatgcg ctggcagccc
     2521 cgctcgatca attcacgggc caggctggac gcatggcccg gcgcctccgt gaagcgaatc
     2581 tctacgggac ccagatgtcg cgccaggcaa cctgaaatgg ccggccatag ccgcccagtt
     2641 ttccctcccg cggaatgggg atttacaatg acgccggttt tgattggcgg cctccagctt
     2701 tacaacgggc tccgggtcta ctttaccgaa agcccggcta cgtggaaaat gcggccgggg
     2761 tctggaccgc cgcgtactcc ggctgtcaaa ttgcggccgg gccttgggga acgggcaacc
     2821 agtcacccgt ggccgctccg gcggcgaatg tcctccaccg tttcccttag cgtctccttg
     2881 gggtcgcggg tttgaaagcc gagctcacgg cgcgccttct ccggcgtgca gtaccagaac
     2941 agagcggaca tcttgatcga agcgtcatcc agcccgtatt tctttccgag cagcgggtac
     3001 agccggcgca gcacggcggc cccgcgcagc gaagcgcgca aagaaagctg ccttttgggc
     3061 gcccggacac ccgctatttc cgagacggcg gttatcattt cacggaaggt ccagttttcc
     3121 cctgcgagaa gataccgctc tccggggcgg cccgcccgca tagcggcgat caggccggcg
     3181 gccgcgtcgc gcacgtcgac gaaattcagg ccgcccagcg gtatcgccag gatctggcct
     3241 tcgagaaaca atctcacatc gccggtggag gaatttcggt cgtcccccgg acccagcagc
     3301 aggctgggat tgacgacgac aatgggcaag ccatacaggc gcacccacga taacgccagt
     3361 ttctcctcaa aaatcttggc caaataatac ggccattcgg ctactatgtc gtttttatac
     3421 cctgattgct cgtcgtggat caccggctcg cggctcacgg cgatagtccc ggacgaagac
     3481 acgagaacaa ttttctccgt accgcgacgc agggcggccc tacagagatt gcgagcgccc
     3541 tcgatgtgca gccggtagag ttcccgcaca tctcggggat ttcgggaaac gatgccggcc
     3601 aaatgataga cctggcccgc cccggccacg gcgcgatcca cgtcctccgg cgaggtgatg
     3661 tctccgcgga tcacttccac ggcaggatcg ccgtcccacg gggaaggccc gcggcagaac
     3721 acccgcaggc ggatgctcgc gtcaacccgc cgcaattgct cgaccaggtg tttccccaag
     3781 aacccggtcg ctcccgtaac caaaaccggc ttcatgatgg cagcggaagc cgtaggctga
     3841 gcggcggggc gcacctcaga cggacacgtt cttgtggagc cattacaggg ccggaagcgg
     3901 ctgggcgtag ggatagagcc ggctccggct gtctttgatc ccggcgcggc cttgcccaag
     3961 cgcttgcgat cgcccccatc atccgacgat ctccggcgac cccatcacgt cagccatccc
     4021 gatgggcggg atgtgcatga gcgtcacgct cggccggcgg ccaaggtggg attgggccat
     4081 atccagggct tcctccatgc tggcggcggt ctcccatccc aggatgcgcg ccacgtccgg
     4141 gttttccgct ccgaccgcga ttaccttgcc gacgtgagcg cggccgtttt cgccccagta
     4201 ccacatatag aacggatgca cgccgtgata ggcgttgccg ctgcggtaca tgcggatgta
     4261 gtcgggatta cgcgcgaact cctcctcgta cttttgctgg agcacgagcg agtcgcgcgt
     4321 ctccggcaag atccggtgga aaaattcgat gtaagaggga tgatgcagcg gatcgaactc
     4381 atcgtaaagc ggatgagtca gaatcaacac gccgttcttc ttcaccaccg gcatgtggcg
     4441 gtacatattg aagaagtatc cgagcgccag cacttgcacc agcagcgggt tcagaatcga
     4501 gttgacgtta taaggcgaaa taaacggaat gccgaatact accacgtcgg attggccttg
     4561 gagcggaacg caatattgtg cgtaacaata ggcgagcgat ttatcgtggg tcgggtcagc
     4621 ggctccggcg tgcacggcga tcatctcgta agccgccgga acagcgaaaa gaattttgcg
     4681 cttggcaggc ctgggcagcg cccgcagccc cgcttgcgcc aaacgaaaag aagcgcggtc
     4741 gagcgcatta tagcggtcct catttttgat gaagaacggc attttgggat cgaacatcgc
     4801 gttgttgagg accgtctcga tgtggaacac cttcacgttc cgttccacga cctccccgat
     4861 gcggttgcag gagcgcgcaa gggccgagcg cgtgtgatcg aaataggaat cgcagtccag
     4921 aatggtctgt ggcgtatgat gagcctgaag cgtgggataa tcacacaggc cgacgcccac
     4981 cgatttgtgg ccgccgtcca tcgagaccag gttgatattg acatagatca acaggtcgga
     5041 ttcggcggcg cggcggttca gccggacgcg ttccccgcga tcggtaacgc cgagctcgac
     5101 catcccgccc ggagcctcgc catcgtggtt gtaaaggcgc tcggggtaat aagcccggaa
     5161 aaccctgctg ccgactgccc gctcaatttc ctgagccgtc atccgcctgt gaaaggaagt
     5221 agcgacgatg atgtgcacgt catccacgcc tttttccgcg agcaattcga gcagtatcgt
     5281 caacaccgat tcgcgaatgt cggggcgcgg catcttgggc aggggaaggg aaatatcatc
     5341 catgccgatg gtcacgcgca taccgggccg cagcaaggcc gccagcggct ccatttgttc
     5401 gggatgggcc agagcgtacc ggacggcccc ctcgcggtcc cgcaagccgg gtatggaggg
     5461 tttgggataa accacacgcg tgccggcggg cattttgaca tcgaggagat cttcgccgta
     5521 ccacagcagg cgcggagccg catcggcttc aagatgcaca acgcagcccg catccgggac
     5581 cagggcccgg cggccgacgg cattgggagc cgggtcgata tcaagcgggg cgggtatgct
     5641 ggcggatcgc atattactcg ccataagggc tttcacatta tactgagacc caacagcgca
     5701 cgagaattaa cggcgcgaaa gaggagggtc tatatgaaaa aagctccgat tctgcttggg
     5761 atggcggccg cactgtgggt gggctgcggc ggcggcccca gtccgcgccc ggaggcgagg
     5821 acgaacgatc cggctgagat caaggcactc gaggatcgct ttatgacggc gttcaacgcc
     5881 aaagatgtga acgccatcat gagctgctat gtgcccgatg aaagcctgct ggtattcgat
     5941 gtgattcccc cgcgccagta taccggcgca aacgcctacc gaaaggattg ggagggtctc
     6001 ttttcggctt tccccggtcc ggtgaccgcg gaaatcagcg acctggatgt gacagcgggt
     6061 gataccgtcg cctacggtca tagcattcag cacgtggtgt tgaccggcaa agacggcaag
     6121 aagctggata tgaccgcgcg cgttaccgac ggatacaaga agattgacgg caaatggctg
     6181 atcagccatg agcacgtttc cgttcccgta gacctcatga cggccaaacc ggatttgagc
     6241 tccaagccgt agtgaggttg ctagcggctg cgcgccacag ccgctccgga cgcggttcgc
     6301 ttcaggtcca gtatgggcca gccgcgcgct gcggctactc gccgcaggcg gctgtccgga
     6361 tttactgcgt gcgggagtcc gacggactcc agcatcggca aatcggaaag actgtctgaa
     6421 tacgccttcg atagcgcgat atctatatcg taccggtggc aaagccggat caaggcggct
     6481 tgcttctcgg cctcggcgat caacggcgcg gcgatgcggc ctgtcgcccg gccgtcgtcg
     6541 taaaccagcg agttgcagat tacgtcctga aagccgaaat accgcagaac cggcccgaga
     6601 tcaaaatcga gcgctccagt cacaagcaca ggacggtagc cctttgcccg gtccgcgtcg
     6661 accagatccc tggccccggg atagatcgag gggcgaatca ccttttcaaa cagactttcg
     6721 gcctgacttt cgagccactc cttcggcaag ccgcgatatt gccgataaaa gacctcgttg
     6781 aacaaacgcc gggagtagag gtcaagacct atgagaaagg gcgcgctcaa caggagcttc
     6841 gcatactcca gaaccgcccg aagccgcgag gggtggtttc tcacgtagta cacgtagcgg
     6901 gtgacaacat tgccggcgat cagcgtcccg tccagatcat agaaagcagc gcgaatcttc
     6961 atcggcggcc cgggatcatg ccggagcgag caggttctga aaattctttc tgtgccgcca
     7021 ggcgaggaat atcgtcataa cagcggcgat caaagcgtcc ctgctgccta cgaacacaag
     7081 catcgacagt gtaaatgcta cccaggcgga gagcgatccg atcgcgccgg cttccttcca
     7141 tttccggcgg ctacccgcga tgcggacgat cgtaaacagc agcaaagcga tcgccgccca
     7201 gcgaggataa agaaccagca tcaccccggc cgaagtcgcc acgcctttgc cgccattgaa
     7261 tttcaggaaa ggggaatagc agtgccccag caccgcggcg aatccgtaaa gccagccggc
     7321 gagtaaaccg tcgcccggcc gcaagcagaa ccagacagcg agaaagcctt tgcccaaatc
     7381 gcccgcgagc gtcagcaccg cgcgccattt gttaacgcgt agcacattgt tgaatccggg
     7441 attgccggag ccgacgcggc gaatgtcgat accgctgccg agcattgcga ggacggcaaa
     7501 aggaatcgag ccggcaaaaa agcagatcgc caggcgggcg ataaaggcgg gttccatcgg
     7561 gggcatcggt ctcaagtcta ttgccaattc ctggcgatcg cttcccgcgc ttcctctcgc
     7621 agacgaacca tttcgcggcg gtccttgtcc gcagggcaaa tcgcgtcgcc tatgacgatc
     7681 ttagctttcc ggcgcagagc cggatgcccg gccagcatgt gcgaggtccc cagaagcgta
     7741 atgggatgca caggagcgga ggcttccacc gcggcgtgga acggatccag gcgaaagcgg
     7801 cttcgatgcg gcggagcgcc gatcgatacg tcgggaaaca ccagcaccga ttgccctcgc
     7861 tgaagcgcct cgaccatccg ccgcctccac gagccgggcg gcatggcggc gctcctgccg
     7921 gatgcgagca caaaaggccg aagcagggca gccgcgaccc gcgaaattcc gaagagcgcg
     7981 tcgccgccgg caaacaacag cggagggggc aaggcggcgg caagcacaat tgggtcgagg
     8041 cggccgggat ggttggcgac aaagaccgcg gggcgagtcc ggtccagggc ggcggattgc
     8101 acttcgattt cataacgact cagcagcagg aacgttcgcg cgcctgcgct gagaagcgag
     8161 cccgtccacg ggccaaggcg aaggatgcgg gccgcgagac ctgcggctgc tgctatacct
     8221 agcaacaatc cataggaata agcttgccgc agccaggccg cggcgcggcg ggctgccaaa
     8281 ccggagcccg agccggcgtt ttccagcgcc aggcggacga tttgcaccca agccggcggc
     8341 ttgcgggctt tcagctcccc gctcagaaac agcgccttgg tctcgaagcg ccggatcttg
     8401 ccgctggatg ttttgggaat actctgcggc ggaaccaact ggaccctgtc gggcggaatt
     8461 cccagcaccg cttccactgc ttttaggata tccgcctcga tccggcgcag ttcttcactg
     8521 cggctggcgc gagtttcagc gaccaccacc agccgctcgg tgccggtatc ggcgtccgtg
     8581 actccgaagg cggccacgca cccccgccgc acgccggcca cttccgaagc cgctccctcg
     8641 acctcctgcg ggataatgtt gcgaccggac ttgataatgc aatccttgcg acgccccgtg
     8701 atatagacct cgccgccgcc ccagtagccg aggtcgcccg agtcgatcca ggtcccgtcg
     8761 accgtcaccg cggcggtagc ggccggattt cgataatagc cggaggtctt cgaggggccc
     8821 cgaaaaagca cacggccccg cacccgttcg ggcagatcgc gattctcgtc gtcgacgatc
     8881 cggatttcat gtcccggcat cggcctgccg ttggccacga agcggatgac atcggattca
     8941 tgcgggtggg cgggctccgc cacgcccagt tcctcaaaag gcttccggcg gatcgcgtca
     9001 atcaccggcc ggcggccgat cggcggaaag gcgagggcca cggaagactc ggccaggccg
     9061 tagcatggca cccaactctc cggccggaag ccgtaggagg aaaaacgctc cgtaaaccgg
     9121 gccagagtgt cgggcaaaac ggcttcgccg gcgttgatcg ctacgcgcca tgcgctcaga
     9181 tcgaggcctt cgagcatacg atccggaatc ttgcgcgcgc aaagttcgta ggaaaagttc
     9241 ggggcggggc agagcgtccc gcgagcgtca tgcatggccc aaagccagcg ctccgggcgg
     9301 ctcaaaaacg ccagaggcga gaggacggtg atcggagtcg catagtatag gctgaagagc
     9361 catgagccga tcagccccat gtcgtgatac aggggcagcc atgtgacgac gatgtcggtg
     9421 ggccgcacct gtaccgaata gccgatgccc cggatattgg ccagtatgtt ggcgtggctc
     9481 aagacaacgc ctttcgggtc gcccgtgctg ccggaagtgt actggatgaa gccggtgtcc
     9541 gagggagaaa cggccggagc cggccgccct cggcccgcct ggcgaagagc ctccacgctg
     9601 gtgacagcgg ccaggcccgg caagttcaac cgcatgatct gggaaacagc tttcacgcgg
     9661 tcgaacgtga tcaagaagcg gacctccgcg ttattcaaaa tgcgcgtttg gcggcgaacg
     9721 tattcttcga tcttgtccgg ccgggcaggg ggatagatcg gcaccggtat gccgcccgcc
     9781 aggctcacgc cgaaaaacgc ataaaaaaaa tcggcgcacg tcgggagcat gatagccacc
     9841 gtctggtcgc gctcgagacc ttcggcggcc aagcctgccg cgacctccgc cgccgcggcg
     9901 agaagctcac ggtacgtgat gtcctggact gattcctcct ccggcagatg gatatgtacg
     9961 cggtccggct cggcttcagc gtggcgccgc agcacctcgg gcagggtgac cgccgtctca
    10021 ggggcaggag gggcttcgcg ggccggcgga gcaatccggt agcggggctt ggcgccggac
    10081 gcgccgtcaa ccggggcgct cgcgccgctg cggacgat
//
LOCUS       DASREK010000316        35017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1313314, whole genome shotgun sequence.
ACCESSION   DASREK010000316 DASREK010000000
VERSION     DASREK010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 35017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35017
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313314"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3514)
                     /locus_tag="VFA54_17190"
     CDS             complement(<1..3514)
                     /locus_tag="VFA54_17190"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HZS52607.1"
                     /translation="MLRHSKLLLFVCVAVCCAGFLLLKGEPEQLPSGKWLPVGAMTSE
                     RAGACAALLPDGRVLITGGMGPAGALTTAEFFGADGSFTPAAEMGSPRSSHACTTLGD
                     GRILITGGVNNGGALNTAEVYDPATDKWQPAGDLLVARSGHTATLLQDGRVLLAGGDS
                     AGVALATLEIFNPAASSFDLAARSLSSPREKHAAVRLKDGRVLIAGGTDGTNTLASAD
                     VFDPITGVVAPAGKMTVPRMLLSATLQLDGRVFLAGGNDGKNDLASSEIYDPARGSFT
                     ASAPLTAPRSGHRAFRIPNNNSILLAGGTSEGVALQASDLFIPWTGSLRPTGALSNGR
                     LLAAGSVLRRPGAVLLSGGARAASAEVYMTATVMTDKADYQPGDTVLITGSGWTPGET
                     VTLNIVESPDDGDGPHVLTPTADPAGNISAQFPIEANDIGIAFTLTATGGTSGLTAQT
                     TFTDTTHLKAIKVLGAQTPNPVVAGNSATYGTTASNSIEVDFNGNGSCPVTLSATGLP
                     TGATAAFAPGSLTGTGTSSLFSLLTINTTTALSGGTYSFTVRADATGGDCPSESATAS
                     ASLVVSTATTTSVVSSLNPSTYGTGVSFTATVTRTAGTGTPTGSVQFKIDGSPFGSPV
                     PLGAPTTPTSATAVSGSTSTLKAGSHTITAEYTPSGSFGPSTGTVTQTVNPKALTITA
                     TTNTKTYDGTTNAAAVPTVSGLVGSDTVTGLAETYDNKNAGTGKTLTVSPGYTVNDGN
                     SGNNYTVTTVTNTTGVINKAALTITAATNTKTYDGTTSAAAVPTTLGLVGGDTVTGLA
                     ETYDTKHVGTGKTLSVSAYTVNDGNSGNNYTVTTVADTTGVITKAPLTITAVTNTKTY
                     DGTTSAAATPTVSGLQTGDTVTGLAETYDNKNAGTGKTLTVSAYTVNDGNSGNNYTVT
                     TVTNTTGVINQRSLTVSATGVNKVYDGNTNATVTLSDNRVAGDSISTSYANAAFADKN
                     VGVGKTVTVSGVSIAGTDAGNYTCNTTTTTTANISARPLTITAAGVNKVYDGTTTATV
                     TLSDNRVAGDVFTDNYASAAFNNKNAGTGKTVNVSGISISGADAGNYTFNTTTTTTAD
                     ITQRPLMVTATGVDKVYDGTVTASVTFSDNRIAGDVLTVSDTAVFVNKNVGNGKTISV
                     TGISISGADAGNYLLSNTTATTTAN"
     gene            3949..4521
                     /locus_tag="VFA54_17195"
     CDS             3949..4521
                     /locus_tag="VFA54_17195"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZS52608.1"
                     /translation="MVPASQLRAGAAIRHEGQIYRVLAADYRGGQGKMGGVAHVRLKN
                     LATGAVWEHSFRADLKLEDLPVEKQTMEYLYEDGGQCYFMDPDTYEQVGIASSVIGEQ
                     ARFLQPQMRLPVEFVEGRPVSVLFPDYIEIRVADTAAPAHQQQDSTLKTAKLENGVEI
                     MVPQFIKTGDLIRLDVENLKYIERVKAPGR"
     gene            4527..5954
                     /locus_tag="VFA54_17200"
     CDS             4527..5954
                     /locus_tag="VFA54_17200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52609.1"
                     /translation="MGLAGRMTWAALLLGPLAWAQGGTGAVPRAGTFPGRARVPFLWQ
                     VAALERQARKAYQARDWAAAAEAYEAFHASGGGTASSYDNLGVALTNLGRWAQAESAF
                     QKAIDLDPEHRWAYNHLGFVYREEGRYEQAVAMFRRQIAISPKDPYAYRNLAGALTLM
                     GRLEEAEQAAAAEEKYTYERGEVYLDMACDLNSQNHPEQAKKYLEKAKSAGAERGLLA
                     QESAHYFLTLHDYRRAEEQYLKLLEYQPYEPLVALRLGTLYWEAGNLEKAAAAFGRVI
                     NVGEDASVTIRTSANTSKRVSFSDLQNNAGAARSVFGDVPADLARAAMLVRLDGYRRE
                     YRQSKSPAQAARLRAACQELLQQSNPIPVEAWLRDTLGWTLLAESRMAAAEEELERAY
                     ALAPGRRMIAYHLAVALEQSGDTERAVEMYTRSLAALPAAQIECGCEEPDLAAREQAA
                     RAAYAKWRGETEGFDAYRKRLEQQK"
     gene            6096..7241
                     /locus_tag="VFA54_17205"
     CDS             6096..7241
                     /locus_tag="VFA54_17205"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZS52610.1"
                     /translation="MAVIDSLSIFRLTSNASQRVIALGVVLGVCYVARPVVITFTCAL
                     LCAFLLEPPVEWLVRLRLPRALAALIVCLLAAALLAVVGGRFYIRSTVLIQELPRYES
                     SIRSIIENVSQRVQNIESALMRFMPPERQQRIVQVIETRRPRTRTVQPAQPPQPPPVQ
                     EVRLKDDGGFLAKYVLPQLGFFYEFLLFASFVPFLVYFMLSWKDHVRHGFVNLFEMED
                     RQVVHNTLDGIGAMARGFLAGNFLLGLFLAACSCLIFWYLRLPFPFMMGTVSGLLSVI
                     PYVGLPLAMVPPVFSALGAYTSVSYYVIIVSIVAGLHLIALNVLYPKLVGSRVHLNPL
                     AVTLAILIWTFMWGPIGLLLAIPITAGLKAVCDNVPSLRKFGELLGD"
     gene            7299..8885
                     /locus_tag="VFA54_17210"
     CDS             7299..8885
                     /locus_tag="VFA54_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZS52611.1"
                     /translation="MVDRITGAKASIVAAVFVLSAAARPCRAQTPSLDSLKNQAMEAV
                     EARRTFTQVMTDSIFSFSELGFQEFETSKYITELLEKEGFRVTRGVAGIPTAFVAEWG
                     SGQPVIGFMADIDGLPETSQRPGVAYRDPLIPGGPGHGEGHNAGQAVNVTAALAVKEL
                     MQKYKIPGTIRVYPGVAEELLASRNYMVRAGLFKDLDAMLSCHVASDFSTSYGPSGTA
                     LVSTQYSFHGQSAHAAGSPWMGRSALDAVELMDAGWNFRREHLRPEQRSHYVITHGGD
                     QPNVVPPEATVWYFFRELDFQHVSDLHAIGTKMARAAAEMTDTTMTERVLAGTWEAHF
                     NKPLAEALFANIERVGMPIWTEADQTLARAAQKELGVKVEGLRTAVTKLRAPGETGRG
                     SAGSDDIAEVSWNLPTVNLRYPANIPNLVAHHWSSGISMATPIAHKGATAGAKAQAMT
                     ALDLLTRPELLAAARAYFRDQTKEVKWVSLVPEGTQPPIELNKEKMERFRPALEKLRF
                     DPSKYKTYLEQLGVKYPTVR"
     gene            8887..9555
                     /locus_tag="VFA54_17215"
     CDS             8887..9555
                     /locus_tag="VFA54_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52612.1"
                     /translation="MMPSVCALVLLAAAVAAGHKAPRKQPAAPAAAGDPLASLHRVAG
                     PVRIDCSGALETVMLLAELGSTGPAITEFQAQARARFAVYAQHPAVVETSSLLTRGFG
                     YKELAEFSTLLSPAPYFALNESDELAALAKLLPSADLNFNVDRLLGYARLIREFYSDA
                     HMGKFLRESTGEYQQALKQVSAKDLPPGVKVLLSPLAPVPPAGRIEFSRRGGRPANYI
                     VLGG"
     gene            complement(9624..10745)
                     /locus_tag="VFA54_17220"
     CDS             complement(9624..10745)
                     /locus_tag="VFA54_17220"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZS52613.1"
                     /translation="MSFSSLGNRLPDLAALGVVLAAAIGLRLAWRKLFVRGRKPGAIH
                     RWLAEYSATPVFLLIVAIGADVVAVRLAALPEIARLGISSYLTNGAYIFMVLSATWLA
                     YALLRGLSEWYLARFVRTAGNAIDVELIPAFRLTAKALLIFIAATVILGHYNVKLTAL
                     LGAAGFASLVLAVAAQATIANMIAGFTILVDRPFRPGDWVELSDGRVGDVQSIGLRST
                     RILSPDYTLYVVPNSELAKSSVINYSFPSEKLNVRQKLTAPFGTDVALVKKILLDACR
                     AHPEVLPDPAPNALLADVGGASVRIEFNYWIADFRRRGLVADEINAEIYTRFRQQGIC
                     LAAPTQVQLIADGSPSGAESAASPNAGRETKRPREVSLP"
     gene            11001..11369
                     /locus_tag="VFA54_17225"
     CDS             11001..11369
                     /locus_tag="VFA54_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52614.1"
                     /translation="MKRLLLLVSTMALIVSGALWAQTPSQTSPGSQAHMQGQSWSGKL
                     VDASCHERDPKASCPISATTTSYGIVTSDGSFYKLDSNGNSQVSKELEQKTTKTGSMN
                     ATVTGSLQGDTINVQSVQIQ"
     gene            complement(11425..12465)
                     /locus_tag="VFA54_17230"
     CDS             complement(11425..12465)
                     /locus_tag="VFA54_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZS52615.1"
                     /translation="MYKALSFLLFAAILAGGASGQTPNGNPQREEERRSQVQKDRTAP
                     FVQAEAVESARRDLLLAMQQHGIQLGAALQTLQNEGRELERQARQHAQELKDMVAQIQ
                     ALGFPPASVSYLGVGVEEIDSARAKELKLKEERGVEIRRVEPDSPAEKAGLKEHDVVL
                     EYNGQPVEGVESFTRMVRETPVGRTAKLLISRDGNTQTVTAVIGRRPALPGRPPYTFT
                     MPPIPPIDIPRPVMTLRAARLGLEIESISGQLADFFGVKEGALVRSVEKDSPAEKAGL
                     KAGDVIVKAAGETIASARDLTQALRSGSDRKTVPLQVVRNKKEMTLNLELPERSRSQN
                     RGEGVRIRQDRF"
     gene            complement(12473..13561)
                     /locus_tag="VFA54_17235"
     CDS             complement(12473..13561)
                     /locus_tag="VFA54_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52616.1"
                     /translation="MQTVTGSLAAVSGGRLRITSFGGISVQGEAGSQVRYVARKRVRA
                     GSEAEARKWLEQAAVRASRQGATVVVVASDPQCGHCGFSADLQVTAPRSTDETILATR
                     GGSLQAYDLDGRVNAQTAGGSIQMDRIGKSVRAETAGGSIKLGSIGGPVRCETAGGAI
                     QLGTAKGDAVLTTNGGSIDADEVGGSLRAETAGGSIHVRRVAQSVNAETAGGSILLGQ
                     IGGSVNAETAGGSITVEAAPGGVHAENAAGSIRLLDVAGALRASTAAGNIWAQLMANH
                     PLADSTLDASAGSIVVLIPDGARLTLRASVDVASTLNRIRCEFPGIVVRMDQGSGPRG
                     LVAEGMLNGGGPVLRIRNTTGSIEIRRR"
     gene            complement(13883..14833)
                     /locus_tag="VFA54_17240"
     CDS             complement(13883..14833)
                     /locus_tag="VFA54_17240"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZS52617.1"
                     /translation="MSCEWVEKQISWYLYNELDERERAEVEEHIETCESCSARMERER
                     AFLAKLDARDRLEPSPALLAESRHDLMRSIYRADRLHWEGRESASTRGAARPMWLSMR
                     LWWQPVAATCLVAAGFFGGWSLRSTPQFLSTPQPAAASFSASDAAAGGISGVSLDLQG
                     GKVQISFDETRRKSFSGSLEDPQIQKFLIFAAKNYGNPGVRLDTVDILKERAGDGEIR
                     DTLLYLVANDANAGVRLKALEALKPYARDSRVRQALIGVLTNDDNPGMRVQAIDLLTE
                     ARDRSLVGVLQGVAQREQNNYVRMRCQNVLQEMNASTETF"
     gene            complement(14957..15577)
                     /locus_tag="VFA54_17245"
     CDS             complement(14957..15577)
                     /locus_tag="VFA54_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS52618.1"
                     /translation="MRAVTLPGISSVKSTPASALSQDAALIRAAQQGDHQAFEQLVRL
                     YDQSVLRLALNLLRSEEDARDAYQEAFLKAYRNLGSFRFECSFYTWLYRIVTNICLDH
                     LRKRTIRREESPVIETRNGARDLFDAVEDGRAEAHPERDLLRRELARRIEDALSRLAP
                     RERLVFELKHYQGLRLRVIGEMLDTTEETAKNCLYRATQKLRAALG"
     gene            complement(15655..16083)
                     /locus_tag="VFA54_17250"
     CDS             complement(15655..16083)
                     /locus_tag="VFA54_17250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZS52619.1"
                     /translation="MKPMPLLLLALILSLPALAQKKIISPPEFPTGSPFSPGVLIGRT
                     LYVSGQTGSDLKTNKLPPNFQDEVRQALDRIGLVLKAAGMDYSDVVSATVYLTDFNLF
                     DQMNQVYTSYFKTNRPARATVQVAGLARGSRVEIMVTASK"
     gene            16301..17014
                     /locus_tag="VFA54_17255"
     CDS             16301..17014
                     /locus_tag="VFA54_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HZS52620.1"
                     /translation="MKIQEQEQNVLRAAANGNSFRYLDVITGLFVAVLLISNVVGQKI
                     WRLGPLDISAAQMLFPVSYIFGDVLTEVYGYARSRRVIWIGFFANILMAVVGWIAVRL
                     PPSPEWPNQQAFAAVMTFVPRMVAASLIAYWSGEFVNSYVMAKMKLFTNGRWLWSRTI
                     GSTVVGQAVDSALVVGITFASVMPLRALVRVTLSIYAVKVVYEVIATPATYLVVNWLK
                     RVEGMDAYDRGTNFSPFAI"
     gene            complement(17106..18665)
                     /locus_tag="VFA54_17260"
     CDS             complement(17106..18665)
                     /locus_tag="VFA54_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52621.1"
                     /translation="MFLRYIAGVRRYLEHPLSAEESRRLVEEQHRNRDQSFLRLLQRS
                     VFQKPRSPYLALLRRARAEYEDVARIVQSDGLESALERLYDAGVYVTLDEFKGRKPIV
                     RAGLEFPVRSHDFDNPFLRRHFEGSTGGTRGVPRRLIIDLDLLVYDAACQHLFHEAWG
                     LAGRPMALWRPAPPYVSGIKKPLLHAKVGQPLSRWFTQDRLEYSPRRLKYAVLTHSTV
                     LAGRLSGVALPAPEHVPLTAALRIANWLVMQRQSGTPAYLDTLVGCAVRICSAAKENG
                     LDISGSFFRLGSEPYTPAKARIVRDSGVQAGCFYAMAETGPVGVPCPDAEAVDDVHLL
                     SGKVALIERRTSLRPAGASVSGFHFTSLLASSPKILINVETGDYGVLVRRRCSCPFGT
                     LGFNDHLHSIRSHEKLTSEGVAFLGTDLFALVEEVLPRKFGGSPTDYQLVETERDGLP
                     RVEIVVSPRVCGVVEASVRRAVLDYLGSRNDSSRTMAKIWDEGQTLRVVRREPAATAG
                     GKILPLHIQRE"
     gene            18889..19356
                     /locus_tag="VFA54_17265"
     CDS             18889..19356
                     /locus_tag="VFA54_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52622.1"
                     /translation="MSKSGTKSKSTRKSTPANARLDRLFRQLENEFQRPGYPIDAQRV
                     FELMSEIHRRYGADKSAIGRKDRLLVNRITELANDAAAVMDSLWAVWIDLVRCLVLTN
                     ITFWGLSEETSGPPLPQLPHPWPPPPKRRREERKGKNSSRLREEARRRSPATA"
     gene            complement(19532..20113)
                     /locus_tag="VFA54_17270"
     CDS             complement(19532..20113)
                     /locus_tag="VFA54_17270"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HZS52623.1"
                     /translation="MARTAFWIITLAGLSPLYLRLFDSRHRRPFVAWSRWVILIRFAE
                     LTTVFLFLAVMVGRHWDFPLTRFSAAAAAAGLILAAWGAFLTSWAKIRLGAQFSTTLG
                     VKQGHQLISAGPYAWVRHPIYTGLLLVLLGGALVYNSGAVLVLLAAPFCCFFYCQSAV
                     EEKLLLAHFGDEYRRYRARTGRLLPRIRVRSSG"
     gene            20216..20680
                     /locus_tag="VFA54_17275"
     CDS             20216..20680
                     /locus_tag="VFA54_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52624.1"
                     /translation="MRLALVLTLEGLLVLAGLVFAQNSQYQELATIKQIMAATQGPAM
                     NALVAFNKAGGPKDDKEWEQAGADAAVLAETSQLLLMGARPAHEAVKKPENGDARDVW
                     VKSSQRLQTAALASAKAAEAKDLDAWKASVNQLGGACRSCHTIHRKPTKKSE"
     gene            complement(20812..21870)
                     /locus_tag="VFA54_17280"
     CDS             complement(20812..21870)
                     /locus_tag="VFA54_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZS52625.1"
                     /translation="MSAVQSPPASAGPAKKTNRIGLDIVGYKGSKTTLCAGCGHNAIS
                     ERIVEAFFDMGVAPWRVVKLSGIGCSSKSPAYFLGMSHGFNAVHGRMPSVATGAVLAN
                     RTLLAIGVSGDGDTASIGIGQFVHLMRRNMPIIYIIENNGCYGLTKGQFSATADIGSK
                     LKTGVVNDLPPIDTCALAIELGATFVARSFSGDKKQLSALLHAAISHKGTVMLDVISP
                     CVTFNDHEGSTKSYSYMKDHEEPLHDIDFVPYFEDIEVEIEPGTTRDVVMHDGSHLRL
                     KKLADDYDASNKITALRVLDEAARKGEVVTGLLYLDTSRDNFLDLLNLIDEPLAHLDE
                     KRARPPESALLEIMEELR"
     gene            complement(21867..23708)
                     /locus_tag="VFA54_17285"
     CDS             complement(21867..23708)
                     /locus_tag="VFA54_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZS52626.1"
                     /translation="MSSVETLPAQYVSSSGGEPVVNDFSIQVATVNGSGSQTANIVLL
                     RSLFQMGIPVSAKNLFPSNIAGLPTWYTIRANKRGFIGRKKETDVMVCMNPETAQEDV
                     RNLNPGAAVVYDAPLELNKLRSDLHFYPVPFDKLVAPVCPDPKLRKLVRNMIYDGILV
                     QLLGIEIEEVHKALFKQFKKKVKAAELNWKAVQAGVEYARQNLTKTDPYRVERMDAKG
                     NKIIIEGNTAAALGSIFAGVTVVTWYPITPSSSLCETLIEYARRYRIDPETGKATFAI
                     VQAEDELASIGMAIGAGWAGARSMTSTAGPGISLMSEFVGLGYYAEIPTVIFDVQRVG
                     PSTGLPTRTSQGDILSTAFLSHGDTKHPLLFPCNPEECFEMAMDAFDLAEELQTPVFV
                     MLDLDLGMNYWMAEPFRYPTRPFKRGKVLTAEDLERLGGFARYKDVDGDGIGYRTLPG
                     TEHPQAAYFVRGSGHNEKANYSERPDDYIRNMDRLNRKFETARSMVPRPVVDLTPGAR
                     IGLIGAGTTHWALVESRDQLQSECGIAASYFRLRAFPFNQELTDFIRKHERVYVVEQN
                     RDAQLLDLMRLDVEPSLHPRLRSVRYYGGLPIDARTITEDIIQQEGL"
     gene            23893..24516
                     /gene="thpR"
                     /locus_tag="VFA54_17290"
     CDS             23893..24516
                     /gene="thpR"
                     /locus_tag="VFA54_17290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZS52627.1"
                     /translation="MSWVHFYIVIIWFHNLGMRLFTAIDPSPTVQSNLERLLEKLRPA
                     AHIRWSRPEGLHLTLKFIGEWPGDRLPELQEALSSVAFEPFDLAASGLGFFPNARSPR
                     VFWVGIQGPPALPRLAAEIEEALEPLGVAREKRAYSPHLTLARIENRTPLGRLHEAIR
                     QLGTADCGTFRARSFYLYESRLAPGGSIYTKIREFPGLGEKMPAGGL"
     gene            24567..25190
                     /locus_tag="VFA54_17295"
     CDS             24567..25190
                     /locus_tag="VFA54_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS52628.1"
                     /translation="MRQWVTKDMEAAAALEHDAQLMLRVKEGDSISFERLLQKYRASV
                     IHYLYRMVQSQSVAEELAQEVFLRVYRSRSSYEPAAKFTTWLFRIATHLALNSLRDHR
                     NERSQESLDGDADEWPRFQVADNRKNREQEMIAEARLEEVRRAIAELPGKQRAAVLMH
                     KYQDMDYAQIAGVLGCSTSAVKSLLFRAYETLRGRLSHLAQSRAALL"
     gene            25203..25685
                     /locus_tag="VFA54_17300"
     CDS             25203..25685
                     /locus_tag="VFA54_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52629.1"
                     /translation="MNCQDIVSEIIAGVGRETSAEVKLHLASCEACSNVYRQQEDLWR
                     QMDAWEAPEISAGFDRRLYARIGRRASSPWTPLGGLLGLAHPTRLPFAAALACMLLLA
                     TAVIERDRHMALPAEPAAAVYPVEKEDLKQIETALDDIQMLSDFEALPVGQIRSQGKS
                     "
     gene            25685..26254
                     /locus_tag="VFA54_17305"
     CDS             25685..26254
                     /locus_tag="VFA54_17305"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HZS52630.1"
                     /translation="MWARGKFLLPLAACAALLSQAAAQQRQALPPPRAARPRTALPRA
                     AVQARVRARQNMVDHLMSLSPEEQRRFMRTNPQFRSLPPLQQQRIQQRLQQFNSLPPQ
                     RQQALRERYELFRQLPAEQQDRARELYRQWMRYPPDRRQELLQGFRRLRDAPPEERQK
                     YLQSEEFRNRFSESEQETLKGLAELLPGG"
     gene            complement(26256..28385)
                     /locus_tag="VFA54_17310"
     CDS             complement(26256..28385)
                     /locus_tag="VFA54_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZS52631.1"
                     /translation="MHQPYYKDLMTGEYRLPWTRLHALKDYYGMVKILDEFPAMRQTF
                     NLVPSMVAQIEEYARGEAHDPFLIAALTRAEDLSTDQRLLIMQYFFMANREKLIYRYP
                     RYRELYERWEGAGRDPGRAARYFSAQDYRDLQVLSQLAWFDEEFLDHDPQVRALVNRA
                     SDYRVEDQQTMARKQQEILSCVMKAYIHAARRGQIEISTTPFYHPILPLICDSDIAAE
                     AHPGLSLPRRFRRPEDAREQLLRARAYIAQRLGSPPVGLWPSEGSVSNEALALAADAG
                     FTWAATDQGVLEKTLRTASTGSRGLLYQPFLWQRDGRRLFLLFRDHFLSDLIGFVFSR
                     MSAEEAAADFVRRLSLIGEPFLAADRDCLIPIILDGENAWEYYDRNGRPFLRSLYRRI
                     LDDPRFCAVTVSDAISRLPHGCLDTIAPGSWINANFDIWIGAEEDNRAWNFLNDARDY
                     FDQSSAAGSSQLRPASRELAYEELLIAEGSDWCWWYGPEHDSPNRPEFDELFRGHLSN
                     VYRALGSKPPEELLRPILHKTTQVLSVQPSGYVRPIIDGEISTYFEWIGAGLYRADHR
                     LGAMHGKRFLVQELYYGTDGASFYLRVDFLPTERERLPGMEARVTLKWGGVAHIHLQL
                     GDRGVQVKEFTAASNGARPEPRDPKLVETRFGRVLEMRVPLEALPRGTGKLRFQFSLW
                     NEGLPFDALPLEEWIELDTSIPEQWEV"
     gene            complement(28466..30931)
                     /locus_tag="VFA54_17315"
     CDS             complement(28466..30931)
                     /locus_tag="VFA54_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HZS52632.1"
                     /translation="MNKALCGWWMLLVFAAVMSSRPAPRVQIGLQPDGAFLLHSGWRL
                     TPAGKQIPLSTLPMSMAISPDGKYLLVLNGGYLPPSISVLDVASERETARVAVEDAWL
                     GIAFNRRGDRVFVGGGSQPFVFEFTFAAGELKPARRFPVMKDPRPADHIGDVAVSPDG
                     RFLYVANIFRNAVTVLNAATGYPVSQVATGRRPYRIVFAPDGKTFYVSHWADASVGQY
                     STADGSRLDVIAVGAHPTDLIYLPASKGAEPQDFTARLFVACANTNSVFVLGINGENR
                     ARMIERINVAMTPRAPAGSTPTALAASPDGARVYVACSDNNTVAVIDVTNPQSEVLGF
                     LPTGWYPTAVHVMRDGRIFVANGRGRGGFANPDGPNPSLKGAERRNYVAAIQTGSLSI
                     IPPPSEEQLDAYSRQAQRNSPYTDRKLTEPPPPLPAQIRHVLYIVKENRTYDQVLGDV
                     KEGNGDPSLVLFGENVTPNHHKLAREFVLLDNFYVNADVSADGHNWSLAGIASDYVQK
                     MWPSNYGHRRKIKDFEGGEPAAIPPAGYLWSNAISAGISLRNYGMWIENNKELTGVER
                     IKDPALAPYTDRSALGFSIDYPDQKRADEFLREFNAFVQKGTLPRLLLLRLPNDHTAG
                     TEAGKHTPRALVADNDYALGRIVEAVSRSPVWRQIVIFVLEDDAQNGPDHVDSHRSPA
                     FVLSAYTKRGFVDSTHYTTVSMLRSIELFLGMRPMTQFDAAATPMVNCFTTRPDSRPY
                     TAELPRVNLEERNPPGAAGAAASARMDFSEEDRIDDDELNDILWRAIRGTEPPAATRS
                     YFGRRRQLATAEAGQIRRAERPD"
     gene            complement(31013..32662)
                     /locus_tag="VFA54_17320"
     CDS             complement(31013..32662)
                     /locus_tag="VFA54_17320"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZS52633.1"
                     /translation="MKIALGQINPTVGDLAGNVDLIIRFAREAANRGADLAVFPELSI
                     SGYPPRDLVEKASFLAAVELELERLARETGALGIDVICGFPRRNTSSTGKTAANCLAY
                     LSGGEVRFTQQKMLLPTYDVFDESRNFAPGEQEKVFPAFGRRLALTICEDVWNDKQFW
                     ERQLYASDPVDELVAQGADFLINISGSPYHMHKRPLRREMLAAIARRRKMPVVFVNQV
                     GGNDQLVFDGTSFVLDASGEVRAAAKSFQEDLVLYDAAANAGEMHENFCDELEAVYEA
                     LVVGCRDYVRKCGFQTVLIGLSGGIDSSLTAVVAADAVGRQNVTGVSMPGPYSSPGSI
                     ADARSLAEKLGIRLEVVDITKIYEVFRDALAPVFAGRPADVTEENLQARIRGATLMAL
                     SNKFGSLVLTTGNKSELAVGYCTLYGDMCGGLAVISDVPKTLVYELARVANRRHEGAI
                     PKAVFQKPPSAELRPNQKDSDSIPEYDVLDPILKAYIEENESPECIARRLRRPLELVR
                     KIVRMVDANEYKRQQAAPGIRVTSKAFGMGRRFPIAQAFRE"
     gene            32689..33939
                     /locus_tag="VFA54_17325"
     CDS             32689..33939
                     /locus_tag="VFA54_17325"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HZS52634.1"
                     /translation="MWHFSKVGLLRQFQEWLETGEETEAGLLEICLRRIREADSHIQA
                     WVRVSPQRRCADGPLRGIPFGAKDIFDTEGIPTEFGSPVYAGRQPEADAALVRTLKQR
                     GAVLVGKTHTTAFAYFDPAPTRNPRDSAHTPGGSSSGSAAAVAAGMVPLALGTQTQGS
                     VLRPASYCGVAGFKPTYGILPLQGVLAFAPTLDTAGLFTQTADDMLLAGKALGWDCSE
                     AAPLTLGVPPLPAGAMPEMERGFCGAVAKLRSEGFRVEPIELPENFTELLPAARLIND
                     YEGARTHQERWKTYGSLIGQKLADLVKRGLAIGAEQYAAALDTLERARQQMAKQFEET
                     PVILFPAATGPAPRGLDSTGDPRMNSPWTALGGPAISIPIPGPGLPLGLQLTAARNRD
                     GFLLAAAAKLEETLNRPPAALVHR"
     gene            complement(33986..>35017)
                     /locus_tag="VFA54_17330"
     CDS             complement(33986..>35017)
                     /locus_tag="VFA54_17330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZS52635.1"
                     /translation="LLGAALVIFAWFLEKQRPARGAGARNIPSEPPAWSVRAGLVSAG
                     FALAMALFALVSYPFPARVDRGKLELTALDVGQGEALFLSLPQNQTMLVDAGGLAAFG
                     STAIPMPDVGEEVVSSYLWARSIRALDVVVISHAHYDHIGGLPAVLENFRVKELWIGN
                     NPPSPEYDHLLDLARRRGARISHLTRGETRCLGGVTFEVLGPAPDYAPRSRPSNDDSL
                     VLLTRFGERSFLLTGDIERGSERRLISDGFLCHADVLKVPHHGSKTSTSERFLDEVRP
                     WFGVVSAGADNPYGHPHADVLARLAAYRTRLFRTDQEGAVTLKTDGHRLYVSTYAWEQ
                     RSGWIRELR"
BASE COUNT         6835 a        10774 c        10644 g         6764 t
ORIGIN      
        1 tattggccgt ggttgtggcc gtggtgttgg aaagcaagta gttgccggcg tcggcgccgg
       61 aaatcgaaat accggtcacg ctgatcgtct tgccattccc tacgttcttg ttcacaaaca
      121 cagccgtatc gctgacggtc aacacgtccc cggcgatccg gttgtcggag aaggtcactg
      181 aggcggtcac tgttccgtcg tatactttat ccacacctgt cgccgtcacc atcagaggac
      241 gctgggtaat gtcagccgtg gtggtcgtcg tagtgttgaa cgtgtagttg ccggcatcgg
      301 cgcccgagat ggagatgccg ctgacgttca ccgttttacc ggtcccggcg ttcttgttgt
      361 tgaatgccgc ggaggcataa ttgtcggtga atacgtcgcc cgcgacgcga ttatccgaga
      421 gcgtcaccgt cgcggttgta gtcccatcgt aaaccttgtt cacgccggcg gcagtgatgg
      481 tgagcgggcg ggccgagatg tttgcggtgg tggtggttgt ggtgttgcag gtataattgc
      541 cggcatcggt tccagcaatc gagacaccgc tgaccgtgac tgtcttgccg acgcctacat
      601 tcttgtccgc aaacgccgca ttggcgtaac tggtggaaat cgaatcgccg gcgacgcgat
      661 tatccgagag cgtgacagtg gcgttggtgt tgccgtcata aaccttattg acgccagtgg
      721 cgctcactgt cagcgagcgc tggttgatga cgcccgtagt gttagtcacc gtagtaacgg
      781 tgtagttgtt gccgctattg ccgtcgttga cggtgtacgc cgagaccgtc agagtcttgc
      841 ctgttcccgc attcttgttg tcgtaagttt ccgcaagccc tgttactgtg tcgccggttt
      901 gcaagcccga gaccgtcggt gtggccgctg cgctggttgt accatcgtag gtcttcgtgt
      961 ttgtcactgc cgtgatcgtc aatggggcct tggtgatcac accagttgta tccgctaccg
     1021 tcgtaactgt gtagttgttg ccgctgttgc cgtcgttgac ggtgtacgcc gagaccgaca
     1081 gcgttttccc tgttcccaca tgcttcgtgt cgtaagtctc ggccagaccc gtcacagtgt
     1141 caccgcccac cagccccaac gtagtcggca cggctgccgc gctggttgtg ccgtcgtagg
     1201 tcttggtgtt tgtcgctgcc gtgatcgtca gcgccgcctt gttgatcacg cccgtggtgt
     1261 tggtgaccgt agtcacggtg tagttgttcc cgctgttgcc gtcgttgacc gtgtaacccg
     1321 gcgaaaccgt cagcgttttg cctgtccctg cattcttgtt gtcgtaggtt tcggccaagc
     1381 cggtgaccgt gtcgctaccc accaagcccg acacggtcgg cacggctgcc gcgttggttg
     1441 tgccgtcgta ggtcttggtg ttggtggtcg cggtaatcgt caatgccttc gggttcaccg
     1501 tctgagtgac ggtcccagtg ctcggcccga agctgcctga aggcgtatat tccgccgtga
     1561 tcgtgtgcga tccagccttg agcgtactgg tcgagccgct tactgcggtt gcactggtgg
     1621 gcgtggtagg agcgccgagg ggcacagggc ttccgaaggg cgaaccatcg attttgaact
     1681 gcaccgatcc agtaggcgtt ccggtgcccg cagttctcgt taccgttgcc gtgaaactca
     1741 ccccggtacc gtaagttgac ggattcagcg aggaaacaac gcttgtcgta gtcgctgtgc
     1801 tgacgaccaa agacgcacta gcggtcgcgg attcgctagg gcaatcccct cctgtcgcgt
     1861 cagctcgcac tgtgaacgag tatgtgcctc ccgaaagtgc ggtagtcgta ttaatggtta
     1921 acagcgagaa cagtgagctg gttcctgtcc ccgtcagtga accgggagcg aatgcagccg
     1981 tcgccccggt gggcaatcct gtggcgctca gagtaacagg gcaacttccg ttgccgttga
     2041 aatccacctc aatggaattg gatgccgtgg ttccgtacgt ggcggagttg cccgcaacca
     2101 cagggtttgg cgtctgcgcg cccaacactt tgatggcttt gagatgcgtg gtatccgtaa
     2161 acgtcgtctg agcggtcaga cccgacgttc cgccggttgc ggtaagcgtg aaagcgatgc
     2221 cgatgtcatt cgcctcaatc gggaactggg cgctgatgtt gcccgcaggg tcggcggtgg
     2281 gcgtgaggac atgcgggcca tcgccgtcgt ccggcgattc gacgatgttc aatgtcaccg
     2341 tctctcccgg agtccagccg gagccggtga tcaggaccgt gtcgccgggc tggtagtccg
     2401 ccttgtcggt catcaccgtg gccgtcatgt aaacttcggc gctggcggcg cgcgcaccgc
     2461 cgctgaggag cacggcgccg ggccgccgca gaacgctgcc cgcggccaga agacgtccgt
     2521 tcgaaagcgc gcccgtaggc cgcagggagc cggtccaggg aatgaacaga tcgctcgctt
     2581 gaagcgcgac tccctcggag gtcccgcctg ccagcagaat gctgttgttg ttcggaatcc
     2641 gaaacgcccg gtgtccgctg cgcggcgcgg tcagcggcgc agaggcagtg aagcttcccc
     2701 tggcagggtc gtaaatctcg ctggaagcga ggtcgttctt gccgtcattg ccgccggcca
     2761 ggaaaacgcg gccatccaac tggagagtgg cggaaagcaa catgcgcggg acggtcattt
     2821 tgccggcggg cgcaactact ccggtgatgg gatcaaacac atcggccgag gccagcgtgt
     2881 tggttccgtc cgtgccgccc gcgatgagaa cccggccgtc tttcagccgc acggcggcat
     2941 gtttttcgcg gggcgaggaa agagaacgag cggccagatc aaaggaactg gcggcgggat
     3001 tgaaaatttc gagcgtcgcc agcgccacac cggcgctgtc gcccccggcc aataacacac
     3061 ggccgtcctg caaaagcgtc gccgtgtggc ccgagcgcgc cacgagcaaa tcccccgccg
     3121 gctgccattt gtctgttgct ggatcataga cttccgctgt gttgagagcg ccgccgttgt
     3181 tcaccccacc ggttatgagg atgcggccgt caccgagagt ggtacaggcg tggctgctgc
     3241 gcgggcttcc catttcggcg gcgggggtaa aggagccgtc cgccccgaaa aattccgcag
     3301 tcgtcaatgc tcccgcaggt cccatcccgc cggtgatcag cacgcgccca tcgggcagca
     3361 gagcggcgca cgccccggcg cgttcgctgg tcattgcacc gaccggcagc cacttgccgc
     3421 tcggtaactg ctcgggctct cctttcagca gaagaaatcc cgcgcagcag accgcgacgc
     3481 aaacaaatag caagagcttt gaatggcgga gcatcccctc acctcaggag ccgggcgggt
     3541 gttcagcaat ttcgtaacca tccatacatt cggcggcgat ctgcttgaaa ggcgggatct
     3601 taaagggtgg cggtcctgtt tgcgctgacg ccaagtgtga acgagagtat acgagttgct
     3661 gaaaaagccg aaaccacctg tattcacaat aacaactgcg atatatcgtc ctgtgatcta
     3721 tagtatgtcg ttccgcgata tatcggtcgg tccgagttcc tggccgctga aaaagtagcc
     3781 ggcgacggaa accgggtgcg ctgaggagac ggctctcacc cggttgcgcc aggagacggc
     3841 tcttaccggg cgcgccgctg cgagcctgga agcggcgtgg ggccggacgg ccccagcaaa
     3901 atcgcgccgg cggcggttgt agtacactcg ttgccaggcg gtggggccat ggttcctgct
     3961 tcacagcttc gagccggagc ggcgatccgg cacgagggcc agatttaccg cgtgctggcg
     4021 gccgattatc gaggcggcca aggcaagatg ggcggggtag cccacgtgcg tctaaaaaat
     4081 ctggctacgg gcgccgtctg ggagcacagc ttccgcgccg atctcaaact cgaggatcta
     4141 cccgtggaga agcaaaccat ggagtatctc tacgaggacg ggggtcaatg ctatttcatg
     4201 gatcccgata cctacgagca ggtgggcatc gccagctccg tcatcggcga acaagcgcga
     4261 ttcttgcagc cgcagatgcg gctgccggtg gaatttgtcg agggccgccc ggtgagcgtc
     4321 ctgtttcccg actacatcga gattcgcgtg gccgatacgg ccgctccggc gcaccagcag
     4381 caagacagca ccctcaagac ggcgaaactg gaaaacggcg ttgagatcat ggtgccccag
     4441 ttcatcaaga caggcgatct gatccgtttg gacgtagaga atctcaaata catcgagcgc
     4501 gtaaaggcgc ccgggagata ggcgtcatgg gccttgccgg gaggatgact tgggcggcct
     4561 tgctgctggg gccgctggcg tgggcgcaag gtggaaccgg cgcggtcccg cgtgcaggga
     4621 cttttccggg ccgcgcgcgc gtcccatttc tgtggcaggt ggcggcgctc gagaggcagg
     4681 cccggaaggc gtaccaggct cgggactggg cggccgcggc ggaggcctac gaggcattcc
     4741 acgcctcggg cggcggcact gcatccagtt atgacaatct gggtgtggcg ctcaccaacc
     4801 tgggccgctg ggcccaggcg gaaagcgcgt tccaaaaggc catcgatctt gatcccgagc
     4861 accgctgggc ttacaaccac ctaggctttg tttatcgcga ggagggccgc tacgagcagg
     4921 ccgtggcgat gttccgccgc cagatcgcca tcagcccgaa ggacccctac gcctaccgga
     4981 atctggccgg ggcgctgacg ctgatgggcc ggctggaaga ggccgagcaa gcggcggcgg
     5041 ccgaggagaa atacacatac gagcgaggcg aagtgtacct cgatatggcc tgcgacctga
     5101 actcgcagaa tcaccccgag caggccaaga aatacctgga gaaggccaag tctgcgggcg
     5161 ccgaacgcgg cttgctggcg caggaatcgg cacactattt tttgaccctg cacgattacc
     5221 ggcgcgccga agaacagtac ttgaagcttc tcgaatacca gccctatgag ccgttggtcg
     5281 ccctgcggct gggaacgctc tattgggagg ccggaaatct ggaaaaggcg gccgcggcat
     5341 ttggccgcgt cataaatgtt ggcgaggacg cgagcgtcac gattcgaacc tcggccaaca
     5401 cgtcaaaaag agtcagcttc tccgacttgc agaacaatgc cggcgcagcg cggtccgtgt
     5461 tcggcgatgt gccggccgat ctcgcccgag cggccatgct ggttcggctg gatggttacc
     5521 ggcgggaata taggcagtcg aaatccccgg cacaagcagc ccggctgcga gcggcctgcc
     5581 aggagcttct ccagcagagc aatccgattc cggtggaggc gtggctgcgg gatacgctcg
     5641 gttggaccct cctggcggaa agccgcatgg cggcggcgga agaggaactg gagcgcgcgt
     5701 acgccctggc gcccggccgc aggatgatcg cctatcatct ggccgtggcg ttggagcaat
     5761 ccggcgacac agagcgggcg gttgagatgt atacccgctc gctagccgca ttaccggcgg
     5821 cgcaaatcga atgcggctgc gaagagccgg acctggctgc ccgggaacag gctgcccgag
     5881 cggcgtacgc gaagtggaga ggggagacgg agggcttcga cgcctaccgc aagcgcctgg
     5941 agcagcaaaa atagaccggc gcggcgccgt cgggggctaa ggggaaagcc tgtgcgcccg
     6001 cagccggaac ggggcgctgc tgcggtcaag gaaaaaattg cccgcggggc tccgactggg
     6061 ttagattgaa ggtacgggcg ccgaggcttg gggctgtggc agtaatcgat tctctctcca
     6121 ttttccggct tacatccaat gccagccagc gggtaatcgc gttgggggtc gtgctgggcg
     6181 tctgctatgt ggcgcgcccg gtggtgatca cgtttacctg cgccctgctg tgcgcatttc
     6241 ttctggagcc ccccgtcgag tggctagtgc ggctccggct tccgcgcgcg ttggccgcgc
     6301 tgattgtctg tctgctggct gcggccctgc ttgcggtcgt tggggggcgt ttttacatcc
     6361 gcagcacggt cctgatccaa gagctgccgc gttatgaaag ctcgatccga agcattatcg
     6421 agaatgtctc gcagcgcgtc caaaacatcg agtccgcctt gatgcgattc atgccgccgg
     6481 agcggcagca acgcattgtg caggtcatcg agaccaggcg gccccgcacc cgcaccgtgc
     6541 agccggcgca gccgccgcaa ccacctcccg tgcaggaagt gcggctgaag gatgacggcg
     6601 gctttctggc caaatatgtc ctgccgcagc ttggattctt ttacgaattt ctattgttcg
     6661 cgtccttcgt tccgtttctg gtctacttca tgctgagctg gaaggatcac gtccgccatg
     6721 gctttgtgaa tctgttcgag atggaagacc ggcaggtggt gcataacact ctggacggaa
     6781 tcggcgccat ggcgcgagga ttcctcgccg gcaactttct tttgggactt tttttggcgg
     6841 cgtgcagttg cctgatcttc tggtatctgc ggcttccttt cccgtttatg atggggacgg
     6901 tcagcggctt gctgagcgtc attccctatg ttggcctgcc tctggcgatg gtgccgccgg
     6961 tgttctcagc gctgggagcc tacacctcgg tctcttatta cgtgatcatt gtttccattg
     7021 ttgccgggtt gcatctgata gccctcaacg ttctttaccc caagctggtt ggatcccgcg
     7081 ttcatctgaa ccctctggcg gttacgctgg cgattttaat ctggacgttc atgtggggtc
     7141 ccatcggcct gttgctcgcc attccgatca ccgcaggtct caaagcagtc tgcgataatg
     7201 ttccgtcgct gcgcaagttc ggagaattgc tcggcgatta gccgctacgc aggcgggatc
     7261 gatgcagggc gccgttgcgc ccgctccggg aggattgtat ggtcgacaga atcacgggcg
     7321 caaaggccag tattgtcgcg gctgtcttcg tattgagcgc ggccgcccgt ccatgccgcg
     7381 cgcaaacacc cagcctcgac tctttaaaaa accaggccat ggaagccgtg gaagcgcgcc
     7441 gcaccttcac ccaggtgatg acggactcca tcttcagctt ctcggagctt ggatttcagg
     7501 aattcgaaac atccaaatat attacggaat tgttggaaaa ggagggcttt cgcgttacgc
     7561 ggggagtcgc cggcattccg accgcgtttg tggcggagtg gggatccggc caaccggtga
     7621 tcgggttcat ggccgacatc gacgggctcc cggaaacctc gcagcggccc ggcgtggcct
     7681 accgcgatcc gctgattccc ggcggtccgg gacacggcga agggcacaat gccggccagg
     7741 cggtcaacgt caccgcggcg ctggcagtga aagaactgat gcagaagtac aaaatcccgg
     7801 gcacgatccg cgtttatccg ggggttgccg aggagctgct ggctagccgc aactacatgg
     7861 tgcgcgcagg cctgtttaaa gacctggatg ccatgctgag ctgccatgtg gcaagcgatt
     7921 tttcaacctc ctatggcccg agcggaactg cgctcgtctc gacgcagtac agctttcacg
     7981 gccagagcgc ccacgcagcc ggatcgccgt ggatgggccg cagcgccctg gatgccgtcg
     8041 agctcatgga tgcgggctgg aactttcgcc gcgagcacct gaggccggag cagcgctccc
     8101 attacgtgat cacgcatggc ggcgatcagc cgaacgtcgt tccgcccgag gccacggtct
     8161 ggtatttttt ccgcgagctc gattttcagc acgtgagcga tctccacgcg atcgggacca
     8221 agatggcccg cgcagccgct gaaatgaccg ataccacgat gaccgaacgg gtgctggccg
     8281 gcacatggga agcgcatttc aacaagccgc tggctgaggc gcttttcgcc aacatcgaaa
     8341 gggtagggat gccgatttgg accgaggccg atcaaaccct ggctcgagcg gcgcagaaag
     8401 aactgggcgt aaaagtggag gggttgcgaa cagcggtgac caaactgcgc gcgccgggag
     8461 aaaccggccg cggcagcgcc ggctcggatg acattgcgga ggtttcctgg aaccttccga
     8521 cggtgaacct tcgctacccg gccaatatcc ccaacctggt ggcgcaccat tggtccagcg
     8581 gtatctccat ggcgacgccg attgcccaca aaggggcgac cgccggggcc aaagcgcagg
     8641 ccatgacggc gctcgatctt ctcacgcggc ctgagctgct cgcagcggcc cgggcgtatt
     8701 ttcgtgacca gacgaaggaa gtcaaatggg tttcgttggt gccggaagga acccagcccc
     8761 ctatcgagct gaacaaggaa aaaatggagc gcttccggcc ggcactcgaa aaactgcgct
     8821 tcgatccgtc aaaatataaa acatatctgg agcaacttgg tgtaaagtat ccgacagtac
     8881 gttgaaatga tgccctcagt ctgcgcgttg gtattactgg ccgccgccgt agcggcgggg
     8941 cataaggcgc cccgcaaaca gccggcggct cccgcggccg ccggcgatcc cctcgcctcc
     9001 ctgcaccgcg tggccggtcc agttcgtatt gattgcagcg gagcgctgga aacggtgatg
     9061 ttgctcgcgg agctcggctc tacagggccg gccattaccg aatttcaagc ccaggcccgg
     9121 gcccgttttg ccgtatacgc acagcatccg gcggtggttg aaacatcctc gctcttgacc
     9181 cgcggctttg gctacaagga gttggccgag ttctccacct tgctctcgcc agccccctat
     9241 ttcgccctca acgaatcgga cgaactggcc gccctcgcca aactgcttcc cagcgccgat
     9301 ctcaacttca atgtggatcg ccttctgggc tacgccaggc tgatccgcga attctactcg
     9361 gatgcgcata tgggcaagtt tctgcgcgaa tccacggggg agtaccagca agcgctaaaa
     9421 caggtatccg cgaaagatct gcccccggga gtcaaagttt tgctttctcc cttggcccct
     9481 gtaccgccgg ccggccgcat cgagttcagc cgcaggggcg ggagaccggc gaactacatc
     9541 gttcttggcg gataagccaa tctgaatgcc gggccagatt aggcccagag accgagccaa
     9601 agaagggatg acatgcgcgc cgatcaaggc agagaaactt ctctcgggcg cttggtttcc
     9661 cgcccggcgt ttggactggc agcgctctct gcgccgcttg gcgaaccatc ggcgataagt
     9721 tgcacctgcg tcggcgcggc aagacagatg ccctgctggc ggaatctggt ataaatctcg
     9781 gcgttgatct cgtcggccac gagccctcgc cggcggaaat cagcaatcca gtaattaaat
     9841 tcaattcgca ccgaggcgcc gccaacgtcc gccagcaagg cgtttggcgc ggggtcggga
     9901 agcacttctg gatgcgcccg gcaagcgtcc agcaggattt tcttgaccag agccacatca
     9961 gttccaaatg gcgccgtgag tttttgccgc acgttcagct tttcgctggg gaaggaataa
    10021 ttgatcacgc tggatttggc cagttccgaa ttgggcacga catacagcgt gtaatcgggg
    10081 gacaaaatcc gggtggagcg cagcccgatg gattgcacgt cgcccacacg cccgtcggat
    10141 agctccaccc aatcccccgg gcgaaaaggc cggtccacta agatcgtaaa tccggcgatc
    10201 atgttggcga tggtggcctg ggcggccacg gcaagcacca gagaggcaaa gcccgccgcc
    10261 cccaacaggg ccgtcagctt cacgttatag tggcccagga tgacggtcgc tgcgatgaag
    10321 atcagcaggg ccttcgccgt caggcgaaaa gcggggatca gttccacgtc gatggcgttc
    10381 cccgccgtac gcacgaatct ggcgaggtac cattcggaga ggccgcgcag cagggcatac
    10441 gccagccaag tcgcgctcag caccatgaaa atgtaggcgc cgttcgtgag gtaactactg
    10501 attcccaggc gggcgatttc cggcagcgcc gccagccgca cagcaacgac atccgcaccg
    10561 atggccacaa tcagcaggaa aacgggagtc gcgctgtact cagcgagcca tctgtggatt
    10621 gcgccgggct ttcgcccccg gacgaagagc ttgcgccacg ccagccggag gccgattgcc
    10681 gccgccagca ctacgcccag cgcagccagg tccggaagcc ggttcccgag agacgaaaag
    10741 ctcattgccc ccatcttagc ctgctttcac acaaactgga tcaggcgcga ctgtgattcc
    10801 ccacaccatg cggcaagcaa aattcacata cctggaccga aaatttcccg cccttccgtc
    10861 cggcctcttg ccgagcgcgg atccatcttc cggggcggcc cgtgccggat aaagcacttc
    10921 gcattccggg tgcttcgttg aagttttgct tttcacactg gcacgcctgt tgcgaattcg
    10981 cacgcccgag gaggatagat atgaagagac ttttgttgct ggtttcgaca atggctctga
    11041 ttgtgagcgg cgcgctgtgg gctcagacac ccagtcagac cagtcccggt tcgcaggctc
    11101 acatgcaggg tcagagctgg tcggggaaac tggtggatgc ttcctgccac gaaagagatc
    11161 cgaaggcaag ctgcccgata agcgcaacca cgacgtcgta cgggatagtc acatcagatg
    11221 gttccttcta caagctcgat tccaacggca attcccaggt cagcaaagaa ctggagcaaa
    11281 agactaccaa gacggggagc atgaacgcca ctgtgaccgg ctccttgcag ggagacacaa
    11341 tcaacgtgca atccgttcag atccagtagc cgcgcggcac gcgcgcgggc cgaaagcgaa
    11401 gctcgcggaa ccgcgcgcgt tgtttcaaaa tctgtcttgg cgaattctta caccttctcc
    11461 gcgattctgg ctgcggctgc gctccggcag ctcgagattg agcgtcattt cttttttatt
    11521 gcgcaccacc tgaaggggaa ccgtcttcct gtcgctgccc gatcgcaggg cctgcgtgag
    11581 atcgcgcgcg ctggcgatag tttcgccggc cgccttcacg atcacatccc ccgctttcag
    11641 gccggctttc tccgccggcg aatccttttc cacggaccgc accagggcgc cttccttgac
    11701 tccgaagaag tcggccaact gcccgctgat cgattcaatt tccaagccca gacgggctgc
    11761 ccgcagcgtc atcacagggc gcgggatgtc aattggcggg atcggcggca tagtgaacgt
    11821 ataaggagga cggcccggca gcgccggccg gcgccctatg acggcggtta cggtttgcgt
    11881 attgccatcc cggcttatca gcagcttcgc ggtccggccc accggcgtct cgcgaaccat
    11941 ccgcgtgaaa gattcgacgc cttccaccgg ctgcccattg tattcgagca caacgtcgtg
    12001 ctccttgagg cccgcctttt cagcgggact gtccggctcg acgcgccgga tctcaacgcc
    12061 tcgttcttct ttaagcttta attccttggc gcgggcgcta tcgatctctt ccacccccac
    12121 gcccaggtaa ctcacgctgg ccggcggaaa cccaagcgcc tggatctgcg ccaccatgtc
    12181 tttgagctcc tgtgcgtgtt ggcgagcctg gcgctccagt tcgcgccctt cattctgtaa
    12241 agtttgtaaa gcggccccaa gctggatgcc gtgttgctgc atcgccagga gcaaatcgcg
    12301 cctggcgctc tccactgcct ccgcctggac gaaaggagca gtccgatctt tctggacttg
    12361 gctcctgcgc tcttcctccc gctgcggatt tccgttcggc gtctggccgg atgcgccgcc
    12421 agccagaatc gcggcgaaca acaaaaatga taaagccttg tacatcggaa tactacctcc
    12481 gtctaatctc aatgctcccg gtggtattgc gaatacgcaa taccggcccg ccgccattca
    12541 acatcccctc ggccactaga ccgcgggggc ccgagccctg gtccatgcgc accacaatgc
    12601 cgggaaactc gcaccggatt cggttgagag tggaagcgac atccacgctg gcgcgaaggg
    12661 tgagccgggc gccatcggga atgagaacga caatgctgcc ggctgacgcg tccagcgtcg
    12721 aatcggccag gggatggttg gccatcagtt gcgcccaaat gtttccggcc gcggtggacg
    12781 cgcgcagggc gccagccacg tccagcagcc ggatgctgcc ggctgcgttt tccgcgtgga
    12841 ctccgccggg cgccgcttcc acagtgatgg atccgccggc ggtctcggcg ttgacgctgc
    12901 ctccgatttg acccagtaga atcgatcccc ctgcggtttc cgcattcacg ctttgggcca
    12961 cgcggcgcac gtgaatgctg cctcccgcgg tctcagcccg caggctgccg cccacctcat
    13021 cggcgtcaat actgcctcca tttgtagtca gcaccgcatc gcctttcgcg gtccccaact
    13081 gaatcgcgcc tcctgcggtt tcgcatcgga cgggcccgcc gatggacccc aatttgatag
    13141 agccgccggc cgtttccgcc cggacggact tgccgatgcg atccatctga atggaaccgc
    13201 cggcggtctg cgcattgacg cggccgtcca gatcataggc ctggagcgaa ccgccgcggg
    13261 ttgccaaaat cgtttcgtcc gtggaccgcg gcgcggtcac ctgcaaatcc gccgagaatc
    13321 cgcaatggcc gcactgggga tccgacgcca ccacgaccac cgttgctcct tgccgcgagg
    13381 cccgcactgc ggcttgctcg agccatttcc gggcttccgc ctccgagccg gctcgcacgc
    13441 gcttccttgc gacgtaccgc acctggctcc cggcttcccc ctgcacagag atgccgccga
    13501 acgaggtaat ccgaaggcgg cctccggaga ctgccgcgag gctgccggtg actgtctgca
    13561 cccagtatct cccttcgcga acaatcggcg tttcggcctg cgctttggcc ggagcaggaa
    13621 gaagaaggaa ggcgagcggc aagaagccga gcaacccaaa agtcgggcat ctcaggaagc
    13681 cggcggccgg cgattttgtg aatcgcccct gtgctgccgc aactgctccc gagggccggg
    13741 catgctccgc gcgcgcttgc tcaagcgccg gccgcggggc cgaatcgccg gccatttctt
    13801 gaagcccaaa gttcttcata accccaatca ccattctttc cgctgccgtt gcgctttcaa
    13861 tgtgaaagcc tccggagcgc ggttagaacg tctcagtgga cgcattcatt tcctgcaaca
    13921 cgttctggca ccgcatgcgg acgtaattgt tctgctctct ttgcgccacg ccctggagta
    13981 ctccaaccag actgcggtcg cgggcttcgg taagcaagtc gatcgcttgt acgcgcattc
    14041 ccggattgtc atcattggtc agcacgccga tgagagcctg gcgcaccctg gaatcgcggg
    14101 cgtaaggctt cagcgcctcc agggccttca gccgcacgcc tgcattggcg tcgttcgcaa
    14161 ccaaatacaa cagcgtgtcg cggatctcgc cgtctcctgc gcgctccttc aagatgtcca
    14221 ctgtgtccag ccggacgccc gggtttccgt aattcttggc cgcgaagatc agaaattttt
    14281 ggatttgtgg atcctccaga cttccgctaa agctcttccg ccgcgtttca tcgaacgaga
    14341 tctgcacttt gccgccctgg aggtccaaac tcaccccgga gatgccgccg gccgcggcgt
    14401 cgctcgcgct gaaggatgcc gccgccggtt ggggcgtgga gaggaactgc ggcgtggagc
    14461 ggagcgacca gccgccgaag aagccggcgg cgaccaggca ggtagccgct accggctgcc
    14521 accagaggcg catggaaagc cacatcggcc gcgccgcgcc tctcgtgctc gccgactccc
    14581 gcccttccca atgcaagcgg tccgcgcgat aaatggatcg catcaggtcg tgccggctct
    14641 ccgccagcag ggcgggagaa ggctccagcc ggtcccgcgc atcgagcttc gccaggaaag
    14701 cccgttctct ttccatccgc gccgagcagg attcgcacgt ctcaatgtgt tcctcaacct
    14761 cggctcgctc ccgctcatcg agctcgttgt acaaatacca gctaatctgt ttctcaaccc
    14821 attcgcagct catagacagc tccgcaaggc gtctgtacgg gcgaaagaga gcgactcgca
    14881 tacggcggtc cccgcccgct ccggaaggct cttcgatctt tcgcggctca tctcgcagcc
    14941 ccttcactcc agctcatcaa cccagcgcgg cccgcagctt ttgggtggcc cggtacaagc
    15001 aattcttagc agtctcctcg gtggtatcca gcatttcgcc gataacccgc agccggaggc
    15061 cctgataatg tttcagctcg aagacgagcc gttcacgagg cgccagcctg gacagcgcgt
    15121 cttcgatacg ccgggccagc tcccgccgca ggaggtcgcg ctccgggtgg gcctctgcgc
    15181 ggccgtcctc caccgcatcg aaaagatccc gggcgccgtt ccgggtctct ataaccggtg
    15241 actcttcccg ccgaatagtg cgctttcgca ggtgatcgag gcagatgttg gtgacgatcc
    15301 ggtaaagcca ggtgtaaaag ctgcactcga agcggaagct tccgagattc cgataagctt
    15361 tcaaaaacgc ctcttgatag gcgtcccgcg catcctcttc cgagcgcagg agattcaacg
    15421 ccagccgcag cacgctctga tcgtagaggc gcaccaattg ctcgaaggcc tgatggtccc
    15481 cctgctgcgc ggcccggatt aaagccgcgt cctggctgag cgctgacgcc ggtgtactct
    15541 tcacgctgga aattcccggc agcgttacgg ctctcactct cccaatgcct tcacctacaa
    15601 gctgatggac gcagagccgc gcaaaaggta agcggcgttt cccctcgctg gcgcctattt
    15661 gctggcggta accatgattt ccacgcgaga gccccgcgcc aggcccgcca cctgcaccgt
    15721 cgccctggct ggccggtttg tcttgaaata gctggtgtac acctgattca tttggtcgaa
    15781 gagattgaag tcggtgagat agacggtcgc gctgacgaca tccgagtaat ccattcccgc
    15841 ggcctttaat acgaggccga tgcgatcgag cgcctggcgg acttcgtcct gaaaattcgg
    15901 agggagttta ttcgtcttga gatccgatcc cgtttgcccc gaaacataga gagttctgcc
    15961 gatcagcacg ccagggctga aaggcgagcc ggtgggaaat tcgggcggag aaatgatctt
    16021 cttttgagcc agcgcgggca gcgataaaat cagcgccagc agaagcaaag gcatcggttt
    16081 catgagcagc ctcctttttc gctgtgtaag ccatagctta gcaagtcccc tcttgctctc
    16141 cgagcctccg gaaggacaca ggagcttctt tcggggcaat gcagcggcca gcagacaaga
    16201 tctcccgcgg ttaaccggcg cggggcatcc agcccggcgc acaatgcagc ctgtccgaac
    16261 cgccattcta cacaggcggc cggagttata aactggcgat atgaagatcc aggagcagga
    16321 gcaaaatgtt ttgcgcgccg cggccaacgg caattcgttc cgctatctgg acgttatcac
    16381 cggcctgttc gtcgccgtgc tgctcatctc gaacgtcgtg gggcagaaga tctggcggct
    16441 ggggccgctg gatatcagcg ccgcgcagat gctgttcccg gtcagctaca ttttcggcga
    16501 tgttttaacg gaggtctacg gctacgcgcg ttcgcggcgc gtgatctgga tcggcttctt
    16561 tgccaacatt cttatggccg tcgtgggttg gattgccgtg cgcctgccgc cctcgccgga
    16621 gtggcccaat cagcaggcat tcgcggcggt aatgacgttt gtgcctcgaa tggtggcagc
    16681 cagcctgatc gcttactggt cgggcgagtt tgtaaattcc tacgtgatgg ccaagatgaa
    16741 actgttcaca aatggccggt ggctatggtc ccgtacgatc ggctccacgg ttgtcggcca
    16801 ggcggtggat agcgctttgg tagtgggaat caccttcgcc agcgttatgc cgctcagagc
    16861 cttggttcgg gtgacgctct ctatttacgc ggtcaaggtg gtttatgagg tgatcgctac
    16921 gcccgcgacg tatctagtgg tcaactggct caagagagtg gagggtatgg atgcctacga
    16981 ccgaggtaca aacttcagcc cctttgccat ttgaaaggcc aaggctgggc ttcgctcctc
    17041 aagcgccggc gccctttaca aacagagtct ctctctaaca gattggttcg atgccactca
    17101 cgctgctact ctctctggat atgcagcgga agaatcttgc ctccggctgt ggccgccggc
    17161 tccctgcgga cgacgcgcag cgtctggccc tcgtcccaga ttttggccat agtgcgagaa
    17221 ctgtcgttcc gcgagccgag gtagtcaaga accgcccgcc ggacgctcgc ctcaacaaca
    17281 ccgcacaccc ggggactgac gacgatctcc accctgggca gcccgtctct ttccgtttcc
    17341 acgagttgat aatctgtggg gcttccccca aatttccgcg gcaggacctc ctccacaagg
    17401 gcgaaaaggt ccgttccaag gaatgcgact ccctcgctgg tcagcttttc gtggctgcgt
    17461 atcgagtgga gatgatcgtt gaagccaagc gtcccaaaag gacaggaaca ccggcgccgc
    17521 accagcacgc cgtaatcccc tgtctccacg ttgattagga ttttcggact gctggcgagc
    17581 agcgatgtaa aatgaaagcc gctgacggac gcccctgccg gacgcaatga tgtgcgccgc
    17641 tcgatcagag cgactttgcc gctcaacaga tggacgtcat ccacagcctc cgcgtccggg
    17701 caagggacgc cgaccggacc agtttccgcc atcgcataaa aacagccagc ctgaacccct
    17761 gaatcccgca caatgcgggc cttagcaggc gtataaggct cgctgcctag acggaaaaag
    17821 cttcccgaga tatcgaggcc gttttccttc gctgcggagc agatcctgac ggcgcagccc
    17881 acgagcgtat ccagataggc gggagttccc gattgccgct gcataacaag ccagttcgct
    17941 atccgcagcg ccgcagtcaa aggcacatgc tccggcgccg ggagcgcgac gcccgatagg
    18001 cggccggcca ggacagtaga atgcgtgaga accgcgtatt tcagccggcg ggggctgtat
    18061 tccagacgat cctgggtgaa ccagcgacta agcggctgtc ccaccttggc gtgcaacagc
    18121 ggcttcttga taccggagac gtacggtggc gccggcctcc aaagggccat cggccgcccc
    18181 gccaagcccc aggcttcatg aaacaagtgc tggcaggcgg catcgtaaac cagcaagtcc
    18241 aaatcgataa tcagccgccg gggcactccg cgcgttccgc cggtgctgcc ttcaaagtgc
    18301 ctgcggagaa atgggttgtc aaaatcgtgc gaccgcacgg gaaattccag ccctgcccgc
    18361 acaatcggct tgcgcccttt gaattcgtcc agcgtcacgt atacgccagc gtcatagagc
    18421 cgctccaacg ccgactcgag cccatcgctc tggactattc gggccacgtc ctcatactcg
    18481 gcccgcgccc gccgcaggag ggccagatac ggactgcggg gcttttgaaa gacagatcgt
    18541 tgcagcagcc gcaggaaaga ttggtctctg ttccggtgct gctcttcgac cagccgtcta
    18601 gattcctccg cgctgagtgg atgttccaga taccggcgta ccccggcaat gtatcgcagg
    18661 aacattcgga ggtcatcaag catagaagga ggctcgtgat ttccggctga ttgctttgct
    18721 ggtcaaggcc gttgcctgag ggtccgacaa aggctggtcg aggtcgcttc cgccaagaac
    18781 ttctcaagcc agagggaaat tataaggcca tacgcctact caagaaaccc ccaaggttct
    18841 ctttgccgcg gcccgacccg cctcccacaa ggcgtatact ggccggttat gtcgaaatca
    18901 ggaacgaaaa gcaagagcac gcgaaaatca actcccgcaa acgcccgcct ggaccgcctg
    18961 ttccggcagt tagagaacga gttccagcgg cccggctacc cgattgacgc ccagcgagta
    19021 ttcgagctga tgagcgagat tcatcgccga tatggagcag acaagagcgc cattggccgc
    19081 aaggaccgcc tgctcgtcaa ccggattacg gaactggcga atgatgcggc cgcggtcatg
    19141 gacagccttt gggcggtctg gattgatctg gtgcggtgcc tggtcctgac taacatcaca
    19201 ttctggggac tcagcgaaga aacgagcggg cctcctttgc cgcaactgcc gcatccttgg
    19261 cctcctccac cgaagcgaag aagggaagag cggaaaggaa aaaatagcag ccgcctgcgt
    19321 gaagaagcga ggcggcgttc tccggccacc gcttgatgca ctttcttaag gactgatgga
    19381 gcgcgcgcga agcctctgag accttttcag aatatccccc cgaatccgcg aagcggacgc
    19441 tgtgcagcaa gttccctttc caatgcgggc tgctgttccg caaccgcggg caaccgaaag
    19501 cgcctccccg cagcttagta ccttggccaa atcagccgct gctgcggacc cggatgcgcg
    19561 gcaggagccg gccggtgcgg gcgcgatagc ggcgatactc gtcgccaaaa tgggccagca
    19621 gcagcttctc ctcgaccgcg ctttggcaat agaaaaaaca acaaaacggc gccgccagca
    19681 gaaccagcac ggccccgctg ttataaacca gggcgccacc cagcaggacg aggagcagac
    19741 cggtgtagat ggggtgccgc acccaggcat aaggacccgc cgagataagc tggtggcctt
    19801 gctttacgcc cagcgttgta gaaaactgcg cgcctaaacg gatcttggcc cagcttgtca
    19861 gaaaggcgcc ccatgcggcg aggatcaggc ctgcggcggc tgccgcagca gaaaagcgcg
    19921 ttagaggaaa gtcccaatgc cgaccgacca ttacagccag gaaaaggaaa acggttgtca
    19981 attccgcgaa gcgaatcaaa atgacccagc ggctccaggc gacaaacggg cggcggtggc
    20041 gcgagtcaaa caaccgcaga taaaggggac tgagcccggc cagggtgatg atccagaagg
    20101 cagtacgagc catcggacgg ctgctcattc tctcataaaa aaccggaggt cgcttgcgaa
    20161 gtttgcgcga aacggtataa ttgacttcta ttttgtgaag ggggagggtt tttgcatgcg
    20221 ccttgctttg gtgctgacgc tggaaggttt gctggttctc gcaggtctgg tctttgcgca
    20281 aaattcacag taccaggagt tagccacaat taaacagatc atggcggcta cccagggccc
    20341 ggccatgaac gcgttggtcg ctttcaacaa ggccgggggc cccaaggacg ataaggaatg
    20401 ggagcaggca ggggcggacg ccgccgtttt ggcggaaacg tcgcagttat tattaatggg
    20461 cgcccgcccc gctcacgagg cggtaaagaa accggaaaac ggcgacgcgc gggatgtctg
    20521 ggttaagagc agccagcggc tgcaaaccgc cgcgctcgcc tcggcgaagg cggcagaagc
    20581 caaggacctg gatgcctgga aggcgtcagt gaatcagttg ggcggcgcgt gccggagctg
    20641 ccataccata catcggaagc ccaccaagaa atccgagtaa cgcgcaggaa ccggggctcg
    20701 ccgccggccg aggcctggaa cggcgggtcc ctctgtgccg ccttttcttt gccagcgcga
    20761 aggtaaggcg cggtacggct gacgctccac cgggctcccg gtccggcgct tttaacgcag
    20821 ttcttccatt atctcgagaa gcgccgattc cggaggccgt gctcgcttct catccagatg
    20881 ggccaagggc tcgtcgatca aattcagcag atcgagaaaa ttatcgcggc tggtatcaag
    20941 gtacagcaag ccggtaacta cttccccttt acgggcggct tcgtcgagca cgcgcagagc
    21001 cgtgattttg ttcgaggcgt catagtcgtc cgccagcttt ttcagccgca ggtgcgaacc
    21061 gtcgtgcatg acgacgtcgc gcgtcgtgcc gggctcgatt tccacctcaa tgtcctcgaa
    21121 atacggaaca aaatcgatgt catgcagcgg ctcctcgtgg tctttcatgt agctgtagct
    21181 cttggttgag ccttcgtgat cattgaaagt cacgcatggt gagatcacat cgagcataac
    21241 ggtgcccttg tgcgaaatcg ccgcgtgcag cagcgcgctc aattgctttt tgtctccgga
    21301 aaaagatcga gctacaaacg tcgcccccag ctcaatggcc agcgcgcagg tatcgatggg
    21361 aggcaaatcg ttcaccacgc cggtcttcaa cttcgagccg atatccgccg tcgccgagaa
    21421 ttggcctttg gtcaacccgt agcagccgtt gttctcgatg atataaataa tcggcatatt
    21481 gcggcgcatc agatgcacaa actgcccgat gccgatggag gccgtatcgc cgtcgccgct
    21541 gactccgatc gccagtaatg tacgattggc caaaacggct ccggtcgcca cagaaggcat
    21601 gcggccgtgg accgcgttaa agccgtgcga catgccgagg aaataggctg gggatttcga
    21661 agaacagccg atgccggaga gcttcaccac gcgccacggt gcgactccca tgtcaaaaaa
    21721 ggcttccaca atgcgctcgg aaatcgcatt gtggccgcag ccggcgcaca aagtggtttt
    21781 cgatccttta tagccgacaa tatcgagtcc gatccgattg gttttcttgg ccggtccggc
    21841 gctcgccggc ggcgattgca cagcgctcac aagccctcct gttgaataat gtcctcagtg
    21901 atcgtccgcg catcaatcgg cagcccgccg tagtagcgga cgctgcgaag ccgcgggtgc
    21961 aaggacggct ccacgtccag gcgcatcaga tcgagcaatt gggcgtcccg gttctgctcg
    22021 acgacataaa cccgttcgtg tttgcggatg aagtccgtca attcctggtt gaatggaaac
    22081 gcgcgcaggc ggaaatacga ggccgcgata ccgcattcgc tctgaagctg atcgcggctc
    22141 tctaccaagg cccaatgcgt cgttcccgct ccgatgaggc cgatgcgggc gccgggcgta
    22201 agatccacga ccggccgcgg caccatcgaa cgcgccgtct cgaatttgcg attgaggcga
    22261 tccatattcc ggatatagtc gtcgggccgc tcgctgtaat tcgccttctc gttgtggccg
    22321 ctgccgcgca cgaagtaggc ggcctgcgga tgctcggttc ccggcagagt gcggtagccg
    22381 atgccgtcgc catctacgtc cttataacgc gcaaatccgc ccagccgctc caggtcttcg
    22441 gccgttagca ccttgccccg cttgaaaggc ctggtcggat aacgaaacgg ctcggccatc
    22501 cagtagttca tgcccagatc cagatcgagc atgacgaaaa ccggcgtctg gagctcttcg
    22561 gccagatcga atgcgtccat cgccatttcg aaacactctt ccgggttgca cggaaacagc
    22621 aaaggatgct tggtgtcgcc gtgagagagg aacgccgtgc tgagtatgtc gccctgcgat
    22681 gtacgcgtag gcaggcccgt cgagggaccg acgcgctgca cgtcgaaaat gacagtcggg
    22741 atctcggcat agtaacccaa gccgacaaac tcggacatca aggaaatgcc cggtcccgcc
    22801 gtggatgtca tggatcgcgc accggcccat ccggccccta tcgccatgcc gatcgaagcc
    22861 agttcgtctt cagcctgcac aatggcgaaa gtcgccttcc cggtttccgg atcgatgcga
    22921 tagcggcgcg catattcgat cagcgtctcg cacaaagaag acgagggcgt aatgggatac
    22981 caggtcacca cagtcacgcc ggcgaaaatg ctccccagag cggccgccgt attgccttcg
    23041 ataatgattt tgttcccctt ggcgtccatc cgctcaaccc ggtacgggtc cgttttggta
    23101 aggttctggc gggcgtattc caccccggcc tgcaccgcct tccagttcag ctccgccgct
    23161 ttgacttttt tcttgaactg cttgaagagc gccttgtgca cctcttcgat ttcgattccg
    23221 agaagctgca ccaaaatccc gtcataaatc atgttgcgca ccagcttgcg cagcttcggg
    23281 tcgggacaaa ccggcgcgac cagcttgtca aacggaacag gataaaaatg caggtcggaa
    23341 cgcagcttat tcagctcgag cggggcatcg tagaccacag ccgcgccggg attcaggttt
    23401 cgcacgtcct cttgcgcggt ctcagggttc atgcagacca tcacgtcggt ctcttttttg
    23461 cggcctataa aaccgcgctt gttggcgcgg atcgtatacc acgtcggtag gccggcgatg
    23521 ttggaaggga agagattttt cgccgacacc ggtatgccca tctggaacaa tgagcgcagc
    23581 agcacgatat tggccgtttg acttccggaa ccgtttaccg tggctacctg tatgctgaaa
    23641 tcgttgacta ccggctcgcc gccggatgag gaaacgtact gcgcaggcaa ggtttcaacc
    23701 gatgacattc atatttctcc gagatgataa gcccaacttt cgattatagc ggattcggcc
    23761 tggagtgagt ttccgcgccc cggcgacgcg ccgcgaggac taaggcaatc cgcttagctg
    23821 cgagctccag cgcttgcggc gactggattt tttgcggctg aaaaaggagg cgggctccgg
    23881 aaagccgtgt ttatgtcgtg ggtgcacttc tacatagtta tcatatggtt tcataattta
    23941 ggcatgcggc tgtttacggc gatcgaccca tcgcctaccg tgcaaagcaa tctggagcgg
    24001 cttttggaaa aactgcggcc cgctgcgcat atccgctggt cccggccaga aggtttgcat
    24061 ctcactctga agttcattgg ggagtggccg ggcgatagat tgccggagtt gcaggaggcg
    24121 ctcagttctg tcgcatttga gccctttgac ctggccgctt ccggcctggg gttcttcccg
    24181 aatgcccgct ctcctcgcgt cttctgggtg ggaatccagg ggccgccggc gttgccccgt
    24241 ctggccgcag agatcgaaga ggcgctcgag ccgctgggcg tggcccgcga aaaacgcgcg
    24301 tactcgccgc atcttactct ggcgcgcatc gaaaatcgca cgccgctcgg ccgcctgcac
    24361 gaggcgattc gacaattggg gacggccgat tgcggtacgt tccgggcaag atccttctat
    24421 ctctacgaga gccgactggc gcccggaggg tcgatttaca ccaagatccg cgagttcccg
    24481 ggtttgggcg aaaagatgcc ggcgggcggc ttgtaatagc gccacccgcc cagtcgagcc
    24541 gggcggcccg atgactgcaa ctttctatga gacagtgggt tactaaagac atggaagcag
    24601 ccgcggcgct cgagcacgat gcgcaattga tgctccgagt caaagagggc gacagcatca
    24661 gtttcgagcg gctgctgcag aaataccgcg cctcagtaat ccattacctg tatcgtatgg
    24721 tacaaagcca gagcgtggcc gaagagttgg cgcaagaggt cttcctccgc gtttaccggt
    24781 cgcggtccag ctacgagccg gccgccaaat ttacgacctg gctgttccgc atcgctacac
    24841 atctggctct gaattcgctg cgggaccacc gcaatgagcg cagccaggaa agcctggacg
    24901 gcgacgcgga cgaatggccc cgcttccagg tggccgataa ccggaagaac cgggaacagg
    24961 aaatgatcgc cgaagctcgg ctggaagaag tgcgccgcgc gattgcggag ctgcccggca
    25021 agcagcgggc agccgtgctg atgcataaat atcaagacat ggattacgcc cagatcgccg
    25081 gagtgctggg atgctccact tccgccgtaa aatcattgct gttccgggct tatgaaacgc
    25141 ttcgcggccg gctcagccat ctggcccaaa gccgtgcagc gcttttgtga gggaagaaga
    25201 ttatgaattg ccaagatata gtaagcgaga ttatagccgg agtgggccgc gagacgtctg
    25261 cggaggtcaa acttcatctg gcgagctgcg aagcctgctc gaacgtgtac cggcagcagg
    25321 aggatctctg gcgccagatg gacgcctggg aagctccgga aatctcggcg ggctttgacc
    25381 gccggctcta tgcgcgcatc gggcggcgcg cctcttcgcc ctggacgccg ctcggcgggc
    25441 tgctgggcct ggcgcaccca acccggctcc ccttcgccgc tgctctggcc tgcatgctgc
    25501 ttctcgccac cgcggtaata gagagggacc gccacatggc gcttccagcc gagccagcgg
    25561 ccgccgtcta ccccgtcgag aaagaagatc tcaagcagat cgaaaccgca ttggacgaca
    25621 ttcaaatgct gagcgacttt gaggctctgc ctgtcggcca gattcgatcc caaggaaaat
    25681 cctgatgtgg gcgcgcggca agttcctgtt gcccctggcg gcgtgtgcgg ccttgctttc
    25741 tcaggctgcg gcgcagcagc gccaagcgct cccgccgccg cgcgccgctc ggcccagaac
    25801 ggctctgccg agagccgcag tgcaggcgcg cgtccgcgcc cgccagaaca tggtggatca
    25861 cctgatgtct ctctcgcccg aagaacagcg gcgctttatg cggaccaatc cgcagttccg
    25921 gagccttccg ccgctgcaac aacagcgcat ccagcagcgg ctccagcagt tcaatagcct
    25981 gcccccccag agacagcagg ctttgcgcga acgctacgag ctgtttcgcc aattgcctgc
    26041 cgagcagcag gaccgcgccc gggagctgta ccgccagtgg atgcggtacc cgccggaccg
    26101 gcggcaggag ctgctgcaag ggttccgccg gttgcgggat gctccccccg aggaacgaca
    26161 gaaatacctg caaagcgaag agtttcgcaa ccggttcagc gaaagcgagc aggagactct
    26221 caagggactg gccgaattgc ttcccggcgg ttagatcata cttcccactg ttcgggaatg
    26281 gacgtgtcga gctcgatcca ctcctccagc ggcaatgcgt cgaacggcag gccctcgttc
    26341 cagagggaaa actgaaagcg tagcttgcct gttccgcgcg ggagcgcctc cagcggaacc
    26401 ctcatttcga gcacccggcc aaaacgcgtt tccaccaatt ttggatcgcg gggctcgggg
    26461 cgagcgccgt tgctggcagc cgtaaattcc ttcacctgaa ctcctcggtc gcctagctgc
    26521 aaatgaatat gcgccactcc gccccatttc agcgtcaccc gggcctccat acccggcagc
    26581 cgctcgcgct cggttggcag aaaatcgacg cgcagataaa agcttgcgcc gtcagttccg
    26641 taatatagtt cctgcaccag aaaccgtttc ccgtgcatgg ccccaaggcg atggtcggcc
    26701 cgataaagcc ctgcgccgat ccattcaaag taagtgctga tctccccgtc gatgatggga
    26761 cgaacgtagc cgctgggctg gaccgaaagg acctgcgtgg tcttatgcag aatcgggcgc
    26821 agcagctctt cgggaggttt gcttccgaga gcccgataaa cgttggacaa gtgcccccga
    26881 aacagttcgt caaattccgg gcggttcgga gaatcatgct cgggaccgta ccaccagcac
    26941 caatcgctgc cctccgcgat aagcagttcc tcataggcca gttcacgcga cgccggccgg
    27001 agctggctgc tgcctgccgc gctgctctga tcgaagtagt cgcgggcgtc gttcaaaaag
    27061 ttccaggccc ggttgtcctc ctcggctccg atccagatat cgaaattggc gttaatccag
    27121 gaccccggcg ctatggtgtc caggcagccg tgaggcagcc ggctgatggc atcgcttacg
    27181 gtgacggcgc aaaagcgcgg atcatccaga atgcgccggt agagtgagcg cagaaacggc
    27241 cggccgttgc ggtcgtaata ctcccaggcg ttctcgccgt cgagaatgat aggtattaag
    27301 cagtcacgat ctgccgctag gaaaggctct ccgatcaggc tcaggcggcg cacaaaatcc
    27361 gcagcggctt cctccgcgct catgcgcgag aagacgaagc cgatcaggtc gctcaagaaa
    27421 tgatcgcgga acaacagaaa cagccgccgc ccgtcccgct gccacaggaa cggctgatag
    27481 aggagtccgc gcgatcccgt gctggccgtg cgaagggttt tctcgagcac gccctggtcc
    27541 gttgcggccc aggtgaagcc cgcgtcagcc gccagggcca aagcttcatt ggagaccgat
    27601 ccttcggaag gccacagccc gactggcggg ctgcccagcc tctgcgcgat ataggcgcga
    27661 gcccggagca attgctcgcg ggcgtcctcc gggcggcgga agcggcgcgg aagagacagc
    27721 cccggatgcg cttccgctgc gatatcgctg tcgcagatca ggggcaagat ggggtggtag
    27781 aacggcgtgg tggagatctc gatctgcccg cggcgggcgg cgtggatata cgccttcatc
    27841 acgcaactga gaatttcctg ctgcttgcgg gccatggttt gctgatcctc cacccggtaa
    27901 tcggaggccc ggttgaccaa agcccgcact tggggatcgt ggtccaagaa ttcttcgtcg
    27961 aaccatgcaa gctgggagag cacctgcaag tcccggtagt cttgcgccga aaagtagcgg
    28021 gccgcgcggc cggggtcccg gccggctcct tcccagcgtt catacagctc tcggtatcgc
    28081 gggtatctat agatcagctt ttcccggttg gccatgaaga aatactgcat gatcaaaagc
    28141 cgctggtcgg tggaaaggtc ctcggctctg gtaagcgccg caatcaggaa aggatcatgc
    28201 gcttctcccc gggcgtattc ctcgatttgc gccaccatcg agggaaccag gttgaaggtc
    28261 tggcgcatcg cggggaactc atcgagaatc ttgaccatcc cgtaatagtc cttcagcgca
    28321 tgcagccggg tccatggcag gcgatactct cccgtcatca aatccttgta atagggctga
    28381 tgcatgtgcc aaagaaaaca gaggtggatc tgcggcatgg tgactcggtg cagagcagcc
    28441 gggacgctcc ggctctagag ccagctcagt ccggccgctc ggcgcgccga atttggccgg
    28501 cctccgctgt ggcaagctgt cttcgtctcc cgaaatagct ccgggtggcg gcgggcggct
    28561 ccgtaccccg aatagcgcgc cagagaatgt cgttcaattc gtcgtcgtcg atgcggtcct
    28621 cctccgaaaa atccatacgg gccgaagcgg cggctcccgc ggctcccggc ggattccgct
    28681 cctccagatt cacacgcggc agctcggcgg tataggggcg cgaatccgga cgagttgtga
    28741 agcaattcac cataggcgtc gcggcggcgt caaactgggt catcggacgc atgcccagaa
    28801 acaactcgat gctgcgcagc atcgatactg tcgtgtagtg cgtgctgtcc acaaagccgc
    28861 gcttcgtata agcgctcaaa acgaacgcag ggctgcggtg ggaatccaca tggtccggac
    28921 cgttctgggc gtcgtcttcc agaacgaaaa tgacgatctg gcgccacacc gggctgcggc
    28981 tgaccgcctc gacaatgcgg cctagcgcat aatcattatc ggccacgagc gcccgcggcg
    29041 tgtgcttgcc ggcctcggtc cccgccgtat gatcattcgg cagccgcaac agcaggagac
    29101 gcgggagcgt tcctttctgc acaaaagcgt tgaactcgcg caggaattca tccgcgcgct
    29161 tctggtccgg ataatcgatg ctgaacccca gggcactccg gtcggtgtag ggagcgagcg
    29221 ccggatcttt tatgcgttcc actccggtca gctccttgtt gttttcgatc cacatgccgt
    29281 aattccgcag actgatgccg gccgagatgg cgttgctcca cagatacccg gccgggggga
    29341 tggcggccgg ttcgccccct tcaaaatctt tgatcttacg gcgatgcccg taattggagg
    29401 gccacatctt ctggacataa tctgaagcga tgccggccag cgaccagttg tggccgtcgg
    29461 cgctgacgtc cgcattaaca tagaagttat cgagcagcac gaactcgcgc gccagcttgt
    29521 ggtggtttgg cgtgacgttc tcgccgaaga gcacgagcga cgggtcgcca ttgccctcct
    29581 tgacgtcgcc cagcacctga tcgtacgtgc gattttcctt cacgatgtaa aggacgtggc
    29641 gaatttgcgc aggcagcggg ggcggcggct cggtcagttt ccggtcggtg taggggctgt
    29701 tgcgctgcgc ctgccgggaa taggcgtcga gctgctcttc ggacggcggc ggaatgatgg
    29761 agagcgatcc ggtttgtatt gctgcaacat aattacggcg ctcagcccct ttgagggaag
    29821 ggttcggccc atcaggattg gcgaagcctc cgcggccgcg cccgttagca acgaagatcc
    29881 ggccatcgcg cattacatgc acggcggtcg ggtaccagcc ggtgggcagg aagcccagca
    29941 cttccgactg cgggttcgta acatcaatca ctgcaacggt attgttatcg gaacaggcga
    30001 catacacgcg cgcgccatcc ggactcgccg ccagcgctgt gggcgtgctg ccggccggcg
    30061 cgcggggggt catggccaca ttgatgcgct cgatcatgcg cgctcggttc tcgccgttga
    30121 ttccaagtac aaacacgctg ttcgtgttgg cgcaggcgac gaagaggcgg gccgtgaagt
    30181 cttggggttc ggcgccctta gaggccggca gataaatcaa gtcggtggga tgcgcgccga
    30241 ctgcaattac gtcaagccgt gagccgtccg ccgtgctgta ctggccgacg cttgcatcag
    30301 cccagtggct tacgtagaag gttttcccgt cgggcgcgaa aacaatgcga tacgggcggc
    30361 gtccggtagc cacctgcgag accgggtagc cggtcgccgc gttcagcacg gtgacggcat
    30421 tgcggaagat attggccacg taaagaaatc gcccgtctgg agaaaccgcg acgtcgccga
    30481 tgtgatccgc cggccgcgga tccttcatca ccggaaaccg gcgggccggc ttcaattcgc
    30541 ccgcagcgaa ggtaaactcg aaaacaaagg gctgcgagcc gccccccaca aatacgcgat
    30601 ctccgcggcg attgaaggca atgcccagcc acgcatcttc caccgcaacc cgcgccgtct
    30661 cccgttcgga tgcgacatcc aagacgctga tcgaaggcgg gagatagcct ccgttgagaa
    30721 ccagcaggta cttcccgtcc ggcgaaatcg ccatcgacat cgggagtgtg ctgagcggta
    30781 tctgcttgcc ggccggcgtc agacgccagc cgctatgcag caaaaaagcg ccatccggct
    30841 ggaggccgat ttggacgcgc ggagcggggc gggaactcat caccgccgcg aaaaccagaa
    30901 gcatccacca accgcacaga gctttattca taaaagcact catcagggac gctcaccgac
    30961 gtaaagccgc ctcccgcgcc ccaaaattgt tccactcccg ctcccggaac acctactcgc
    31021 ggaacgcctg agcaatcgga aagcggcgcc ccatgccgaa agccttgctg gtaacacgga
    31081 tcccaggagc cgcctgctgc cgtttgtatt cgttggcatc aaccatccgg acaatttttc
    31141 gaaccagctc cagaggccgg cgcaagcgcc tcgcaatgca ttccggcgac tcattttcct
    31201 caatataggc tttcaatatg ggatctagca cgtcatactc gggaatcgag tcgctgtcct
    31261 tttggttcgg gcgaagctcg gccgacggcg gcttttgaaa aacggctttg ggaattgctc
    31321 cctcgtgccg gcgattggcc acccgggcca gttcgtaaac caatgtcttc ggcacgtcgc
    31381 tgatcaccgc caggccgccg cacatgtccc catagagcgt gcaataaccc acagccagct
    31441 cgctcttgtt cccggtggtg agcaccaacg agccgaattt gttggaaagc gccatcagcg
    31501 tcgccccccg gatccgggct tgcaagttct cttccgtcac gtccgcgggc cggccggcga
    31561 agactggggc cagggcatcg cgaaagacct catagatctt tgtaatatca acgacctcca
    31621 accggatccc cagcttttcg gcgagagacc gggcgtcggc aatgctgcct ggagaggaat
    31681 acggccccgg catgctgacg ccagtcacat tctgccggcc gacagcatcg gccgccacca
    31741 cggccgtcag ggaggaatca atgccaccgc tcaggccgat cagcaccgtc tggaagccgc
    31801 atttgcgcac gtagtcgcgg cagcccacta cgagggcctc gtacacggcc tcgagctcat
    31861 cacagaaatt ctcgtgcatc tcgccggcgt tggcggcggc gtcataaagc accaaatctt
    31921 cctggaagga cttggcggcg gcgcgaactt cgcccgacgc atcgagcaca aagctcgtgc
    31981 cgtcaaaaac aagctgatcg ttgccgccca cctggtttac gaagaccacg ggcatttttc
    32041 gccggcgggc gatggccgcc agcatctcgc gccgcagcgg gcgcttgtgc atgtgatacg
    32101 gcgacccgga aatattgatg aggaaatccg ctccttgcgc caccagttcg tcaactggat
    32161 cgctggcata aagttgccgt tcccaaaact gcttgtcgtt ccagacgtct tcgcaaatgg
    32221 ttagcgcaag acggcggccg aaagcgggaa agaccttttc ttgctcgccg ggagcgaaat
    32281 tccggctttc gtcaaatacg tcgtaggtcg gcagcagcat tttttgctgc gtaaaccgca
    32341 cctcgccgcc cgaaaggtaa gccaggcagt tcgccgcggt ttttccggtg ctcgacgtgt
    32401 tccgccgggg aaaaccgcag atcacgtcaa tgccaagcgc gccggtttcc ctcgccaggc
    32461 gctccagctc aagttcgact gctgctaaga acgaggcctt ttccactaaa tcgcgcgggg
    32521 gatagccaga aatggacagc tccggaaaaa ccgccaggtc cgcgccgcga tttgccgcct
    32581 cgcgggcaaa tcgaatgatc aaatccacat tgcccgccag gtcgccgacc gtggggttga
    32641 tctgtcccag cgcgattttc atggccggcc gctctccccg cgatacatat gtggcatttt
    32701 agcaaggtgg gcttactgcg gcagtttcag gaatggctgg aaaccggcga ggaaaccgaa
    32761 gccgggctgc tcgagatttg cctgcggcgg attcgcgagg ctgattcgca catccaagcc
    32821 tgggtgaggg tctcaccgca gcggcgctgt gcagacggcc cgctgcgcgg cattcccttc
    32881 ggcgcaaaag atatttttga caccgagggc attcccacgg agtttggttc gccggtttac
    32941 gccggccggc agccggaggc cgacgccgca ctggtgagga cgctgaagca gcgcggggct
    33001 gtgctggttg gcaaaaccca cactacggcg tttgcctact tcgacccggc gccgacccgc
    33061 aatccacgcg atagcgcgca cacgcccggc ggcagctcga gcggctcggc ggctgctgtc
    33121 gcggcaggca tggtcccgct agcgctcggg acgcaaaccc agggatcggt gctgcgcccc
    33181 gcctcttatt gcggcgttgc aggcttcaag ccgacctatg gcattctgcc cctgcaaggg
    33241 gtattagcat tcgctccgac tctcgacacc gccgggcttt ttacgcagac tgccgacgac
    33301 atgctcctgg ccgggaaagc cttgggctgg gattgcagcg aagcagcgcc cctgaccctt
    33361 ggtgtgccgc ccctgcctgc gggggcgatg ccggaaatgg agcgcggctt ttgcggcgcc
    33421 gtggcaaagc tccgctcgga agggttccga gtagaaccaa tagaactgcc ggaaaacttt
    33481 acggagttac tcccggccgc gcgcctcatc aacgactatg aaggcgcgcg cacgcatcag
    33541 gagcgatgga agacatatgg cagccttatc gggcaaaagc tggccgactt ggtaaagcga
    33601 ggtctggcaa ttggtgcgga gcagtacgct gcggctcttg acacgctcga aagagccagg
    33661 cagcaaatgg caaagcaatt cgaggagacg cccgtaattc tttttccggc ggccaccggc
    33721 cccgcgccgc gcgggctcga ttcgaccggc gatccccgca tgaattcccc ctggaccgcc
    33781 ttgggcggcc ccgctatttc cattcccata cccgggcctg gtctgccgct ggggctgcaa
    33841 ttgacggcag ccagaaatcg ggatggattc ttgctggctg ccgcggccaa actggaggaa
    33901 actctgaatc gaccccccgc tgcactcgtg catcgatgat agatgtttcc cgtggcgccg
    33961 ggattacatg cagggcttcc ctcattcaac gcagctcccg gatccagccg ctccgctgct
    34021 cccatgcgta ggtcgagacg tacaggcgat ggccgtcggt tttgagagtc accgcccctt
    34081 cctgatctgt ccgaaacaac ctggtgcggt aagcggcaag ccgtgccaga acatccgcgt
    34141 gcgggtggcc atagggattg tcggctccgg ccgaaaccac gccgaaccac gggcgaacct
    34201 catctagaaa cctttcgctc gtagaggtct tgcttccgtg atggggcacc ttcaacacgt
    34261 ctgcatggca gaggaaacca tcactgatca gccgccgctc tgacccgcgc tcaatgtctc
    34321 cggtcagtag aaaacttcgc tcgccgaagc gagtcagcaa aacgagggag tcatcgttgg
    34381 acgggcggct gcgaggggcg tagtcgggcg cgggccccag cacttcgaag gtcactccgc
    34441 ccaggcatcg cgtttcgccg cgggtgagat gggaaatccg agcgcctcgc cgccgggcca
    34501 gatccaataa gtggtcatat tcgggcgacg gagggttatt gccgatccag agctccttta
    34561 cgcgaaaatt ctcgagaacg gcgggcaggc cgccgatgtg gtcgtagtgc gcgtgcgaaa
    34621 tcacgacaac atccagcgca cggatcgagc gcgcccacag ataggaagac acaacctcct
    34681 cgccgacatc gggcatcggg atggcggtac tgccaaaggc cgccaggccg ccggcatcga
    34741 ccagcatcgt ctgattttgc ggcaggctca gaaacagagc ttccccttgg ccgacatcga
    34801 gggcggtcaa ttccagtttt ccgcgatcga ctcgagcggg aaacggatat gacacgagcg
    34861 cgaagagcgc catagctaaa gcgaatcccg cagaaaccaa gcccgctcgc acactccaag
    34921 cggggggttc gcttggaata ttgcgcgcgc cggcgccgcg agccgggcgc tgtttttcca
    34981 gaaaccacgc aaaaatcacc agtgcggctc ccagcag
//
LOCUS       DASREK010000317        23589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1969580, whole genome shotgun sequence.
ACCESSION   DASREK010000317 DASREK010000000
VERSION     DASREK010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 23589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23589
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969580"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..268
                     /locus_tag="VFA54_17335"
     CDS             101..268
                     /locus_tag="VFA54_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52636.1"
                     /translation="MKKLFSMLLVPIVGVALTLAVPTITKAQDQKDESSAPKKKGKKK
                     KKDETKKDNLR"
     gene            313..474
                     /locus_tag="VFA54_17340"
     CDS             313..474
                     /locus_tag="VFA54_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52637.1"
                     /translation="MKRLFTVMLVPIVGVALSFAVPSVSKAQDNGTTKKESKKKGKKK
                     KKDDSGKAN"
     gene            596..1291
                     /locus_tag="VFA54_17345"
     CDS             596..1291
                     /locus_tag="VFA54_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS52638.1"
                     /translation="MRILLVEDELKVASFIRSALEENSYAVDVSHDGEEGCYMAETTD
                     YDLVILDIMLPKMDGFQMLRRLRSRKPGLPVLVLTARGNIEDRVRGLDLGADDYLVKP
                     FALAELIARVRALLRRGVRESTTELRVDDLVLDSARRRVTRAGKPIDLSNKEYSLLEY
                     LMRNANHAVTRGMIAEHVWDSSFDSFTNVIDVYINHLRNKIDRNFGKKLIHSVRGVGY
                     RVSVNDSEQEPVS"
     gene            complement(1460..3067)
                     /gene="aceB"
                     /locus_tag="VFA54_17350"
     CDS             complement(1460..3067)
                     /gene="aceB"
                     /locus_tag="VFA54_17350"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HZS52639.1"
                     /translation="MATPSTEVLREEGIEVLGKASDISSEILTPEALKFLSKLHRKFE
                     SRRQELLQRRVARQQEIDDGKMPDFLPETKSIREKEWTVAPIPKDLEDRRVEITGPVD
                     RKMIINALNSGANVYMADFEDSNSPTWQNNIEGQFNLRDAVRGVISYTSPEGKSYKLG
                     PKLATLLVRPRGWHLVEKHLRVDGQPISGSLFDFGLFFFHNAEERLKRGTGVYLYLPK
                     MESHLEARLWNDVFNFAQDALGIPRGSIRATVLIETILAAFEMDEILYELRDHSSGLN
                     CGRWDYIFSFIKKFRNKPEFVLPNRAQVTMDKAFLNAYVQLLIKTCHRRGIHAMGGMA
                     AQIPIKNDPDANEKALEKVRQDKLREVKAGHDGTWVAHPGLVGVAKEIFDQHMKTPHQ
                     ISNKREDVHISAKDLLAVPSGDITEEGLRVNIDVGIQYLEAWLMGNGCVPIYNLMEDA
                     ATAEISRSQVWQWVRHGAKLNDGRPVNAELIRKVIPEQLEHIRKLIGPKRFDSGKFDL
                     ASKLFEDMMTRKDFSEFLTLVAYDYID"
     gene            3434..5542
                     /locus_tag="VFA54_17355"
     CDS             3434..5542
                     /locus_tag="VFA54_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS52640.1"
                     /translation="MHKPIKYLEKGVSIAAAGMWHAFRFINDRVPESPSFTPRWSDKP
                     LLKSYQKTKPPLGWPRTTDSLCPRCVPEIRKEILDGKKDYKILLNERVGELKAQIIER
                     DGKILMVKDCPKHGHFEDVMSIDVEFFRHLESVFPGRDIRAHNDEKLHNHGSSTIKHG
                     RGSVLTIDLTNRCNMMCDPCFMDANQVGFVHELTWDDIKTLLDNAISIKPRRQMSVQF
                     SGGEPTMSPYFLDAVRYARKVGYNSVQAATNGIEFAKSPEFCRQAAEAGLRYAYLQFD
                     GIGNAANAHRKVGNLFDVKMRAIENLHSNGVEIVPVTTLVNGINNEQVGRIVRFALDN
                     PGKIAFLSFQPVSFTGRDEAVTDERRMAQRYTLSHLAHDVKNQLGIGEPKRDWFPLSF
                     MGTFSDWSDLVHGPGANWGQVSCGCHPNCGVGMAIMVDKHTREAVPVTAFLNADQLAK
                     DVAKVNDWGRGKWLSIAGMTLALLKNYDPFQTPTHFRLFDLLKKLDKSLGATGYNYGD
                     VSGNRSMKDIFERRKDRWNLLFVAGMWFQDLFNYDFRRTEQCIIPYATQEGEISFCAY
                     NTGIGWRNIIEKMHMTATLTKWYEEHGRHEIFAGGKNVKLNSTEHHLVLRDEIVTKEE
                     QHDLDKLGIAKNAREEKIRARDERLRQQQENARMAKLYRQHVLKEPPAPELVQLQPLI
                     ASEAKPQDQPANELVGSCGD"
     gene            complement(5815..6780)
                     /locus_tag="VFA54_17360"
     CDS             complement(5815..6780)
                     /locus_tag="VFA54_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52641.1"
                     /translation="MMPSSITLPLIASRRFPFFWACLLCLLPAASLRAADYPRQWFLN
                     AHNCYPAGDHGADRLERARRSGLSVIEIDLAWSERRRRAVITHTTKLTGSEPTIEAYF
                     WKPMLPELEKMPRGRPGILVIFDFKDHQPGTVKQVYQFLQRRRALITACGKRGDSPAT
                     PLDWRPLTVLLSGDQEAIAMFERLTPPTEPYLAMGNREPPNDNKFQENVANYFPEPAT
                     AFYRVFNFNWAHIEKSRNNQAGDFTPEERNRLIELVEAGRSKGYWLRAWTLNADSTAW
                     SPEDNFGSREALLARWRAAWEAGLDNVATDEYELAGEFLRSLGSR"
     gene            7131..8084
                     /gene="argF"
                     /locus_tag="VFA54_17365"
     CDS             7131..8084
                     /gene="argF"
                     /locus_tag="VFA54_17365"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZS52642.1"
                     /translation="MPAATLTSQIPKVSTARFTTDLDLTSDELAALFLLAARVKRSPR
                     QFAHKLSGRYLALLFEKPSLRTRMTFELAMKQLGGDAVFTDCNGGRIGDREPIQDVAR
                     NLERWVSGIVARTYQQATIDELSRWAAVPVINALSDLYHPCQVLADFFTLQEKFGQLR
                     GLKLAFVGDGNNVAHSLMLTGSRLGVDIAIATPSGYEPNREIFAMAAGFAAAAGCSLR
                     LTNDAFEAVEGAHAVYTDVWASMGQEKESGRRRKDFAGYQVTGKLMAAALPEAAFMHC
                     LPAHRGEEVVDEVMESAASIVFEQAENRLHAQKALLLMMLS"
     gene            8167..9375
                     /locus_tag="VFA54_17370"
     CDS             8167..9375
                     /locus_tag="VFA54_17370"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZS52643.1"
                     /translation="MTKPKKVALAYSGGLDTSVIIPWLREHYGCEVIAVCGNIGQGEE
                     LDGIVEKAKKSGAADVFVEDMREEFVTEYLWRMVRAGAVYEHKYLLGTSVARPLLAKR
                     QVEVALRTGCDALAHGCTGKGNDQVRFELTYKALAPHLAVIAPWREWTIRSREDALDY
                     AAKHNIPVDASTAKIYSRDRNIWHISHEGGALEDPANPAPDDVWMLTRHPWEAPDRPG
                     AVTIGFERGTPVSVDGVTMSGVGILEKLNHLGAEHGVGRIDLVENRFVGMKSRGCYET
                     PGGTLILLAHRELEALTLDRSTHHYKQRLALDYAEMVYNGFWFTPLREALDAFVSKTQ
                     ETVTGEIKLRLLKGNVDVLSRRSPYSLYSLDIASFTMGASYDQKDALGFINLIGLPMK
                     VRAGVMKASQ"
     gene            9372..10772
                     /gene="argH"
                     /locus_tag="VFA54_17375"
     CDS             9372..10772
                     /gene="argH"
                     /locus_tag="VFA54_17375"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZS52644.1"
                     /translation="MKLWGGRFEKGPSAAFERFSGSLRYDYRLWEADILGSQIFAREL
                     ERQGILTADERERICRAFDEMAAEGPGAELAAGAADEDVHTLVIRKLKEKIGEAADKI
                     HTGRSRNEQVSLDFRLWIRDEIRTLRGRLADLMEALLGLAERDGMAILPGYTHLRRAQ
                     PVLWGHYLLSYFEMLARDAGRFEHAYAAADLLPLGSGALAGSGFPFDRERMARELGFR
                     GVTRNSLDAAGDRDFALDFLYAAAVTMLHLSRLAEDWILYSSEEFGFLELGDEVTSGS
                     SLMPQKKNPDSLELIRGKAGRVAGSLLSLMMTVKGLPSAYNRDLQEDKEAVFDAAEQL
                     AAAVEMAVAVAGSVRLNKERAEAAARQSWTTATELAEALARKGVPFHRAHQIVGGLVL
                     ESLRLGRQPADWTPRQLADFAAELDAGMAALLDPRRAVENHALPGGTAPGAVRQAMEE
                     ARRRLGEIRAKSLAVG"
     gene            10864..11373
                     /gene="argR"
                     /locus_tag="VFA54_17380"
     CDS             10864..11373
                     /gene="argR"
                     /locus_tag="VFA54_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HZS52645.1"
                     /translation="MNKKFRQGQIQKIIRSRNIHTQEELAEALQASGIHATQVTLSRD
                     IRELGIVKTPEGYRERQALEGAPAADEAWRAVEEFLRDVRLANNLLVLKTDPGNANPL
                     AVALDRAPWPEIVGTIAGDDTVLVISGDNQSARKLQERLTAALAATSAAAAARAGPRK
                     ERKATGGKP"
     gene            complement(11407..12042)
                     /locus_tag="VFA54_17385"
     CDS             complement(11407..12042)
                     /locus_tag="VFA54_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52646.1"
                     /translation="MRKISSLALAGGLAAVLLVSGLQAQVPDGVKIDTPKVRVLVATE
                     QPHHPSALHEHTMNRVMIYLGGGQMKFTEPAGTVENMPVKKGQVLWSPAAGPHISENV
                     GGEPFQVVEIELKGKPSGSPAPVSKIDPLHVDPKHYSLELENDQVRVLRVRFGPREKG
                     VLHEHTLEHIVVYLNDQARGKAGEVRLDGPMTHTEQNPLDHPVERIAIDLK"
     gene            complement(12076..12894)
                     /locus_tag="VFA54_17390"
     CDS             complement(12076..12894)
                     /locus_tag="VFA54_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52647.1"
                     /translation="MTITRRSFLGGALGAAALARAQEPAGAKPEQGVPVRKAKVVRLF
                     KAPDEHPNALEATRDALWIGGQVTEKAYKVDWKTGRVLHEVQTDSHNTSGIAVGGGYL
                     WMSANGRTERRPPRPTDNDFDEIFQADLETGKTFRRYRPPWPGGSHGIAYVEQTQTLW
                     VTALGIKALAELDWKDNFRVLRVIPVQHDRAHGIDWDNGAMWVLFSSDYVIHKVDAAT
                     GKVLEVVTLVKGQDPDPHGMCFHEGHLYYCDAGLVVGGGSNGSPSAGYMCRVDL"
     gene            complement(13066..14058)
                     /locus_tag="VFA54_17395"
     CDS             complement(13066..14058)
                     /locus_tag="VFA54_17395"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HZS52648.1"
                     /translation="MKTEYTRRQWLATVGTAGLASRLTATPAGNTPGKPLQGAFMILS
                     TPYTPAKAVDYEDLAGEVDFLVRCGVQGLVWPQNASEQSKLTKEERMRGMEVLAKAAR
                     GKSPALVLGVQGRDTQEMLEYARHAEKLEPDGVIAIPPTTAQSLDDYRDYYRALCKTT
                     KRPVFIQTSGGARDVEPTVEFIVELAREFPNFGYIKEEHDPVIPRMRAEIAHRPDPIK
                     RVFCANRGAGLLYELRLGSDGAIIGGVPYADVFARLWRLHQQQKLQEQREVFSKLLLM
                     LNLDERIPGVRPYILKKRGVFKTSVTRQGDYQLTPEAVAEIEYNFEALKPYLAV"
     gene            complement(14250..15230)
                     /locus_tag="VFA54_17400"
     CDS             complement(14250..15230)
                     /locus_tag="VFA54_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52649.1"
                     /translation="MKIKHLPVLALALAGTAYGWGMRGHAVVNRLALQSLPEDGPIFL
                     RVHEEWIAARARVPDTWRQPSEPFLKIIEDPNHGWFKEQFAFLKQIPRSRYEFVLALY
                     DEYLRIKDKDPARARLTNVRWTGTLPYAAMENYERLKSGMRIYRAQQSAGQETRYTEL
                     DLAFYTGWLGHYIADGAQPMHDSIHHDGWRGPNPNGYTRDGSIHARFESSFVDLMGVE
                     AEDLLPIVGPPKLLPDPFDAILQHLDHSAAHVEEIYRLDKQGAFAGKSNEAAKELVNS
                     QLAAAGQLLRDLLYTAWIESSRPARTPPDPSSPSSPRYNPATGSAPAPKP"
     gene            15303..15848
                     /locus_tag="VFA54_17405"
     CDS             15303..15848
                     /locus_tag="VFA54_17405"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HZS52650.1"
                     /translation="MKTRKPKLLTESGLLEYALRALSARALTQGELRVRLQARAERPG
                     DVETVVGRLKQYGYLDDRRFAETFSRLRRENEGFGKFRVLRDLRSRRIAPGLAEKTVA
                     EAYRDAEEAELIDRFLKRKLRYSDRPPALNDPRRLASLYRMLLRAGFTSAKILEALRK
                     LSVQSEWVDSLETTAEEEDSD"
     gene            15845..18490
                     /gene="alaS"
                     /locus_tag="VFA54_17410"
     CDS             15845..18490
                     /gene="alaS"
                     /locus_tag="VFA54_17410"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZS52651.1"
                     /translation="MTSYLSGDQIRAKFLAFFEARGHRVVRSSSLVPANDPTLLFTNA
                     GMNQFKDVFLGREKRNYTRAASAQKCVRAGGKHNDLENVGRTRRHHTFFEMLGNFSFG
                     DYFKREAIDYAWTLVTGDFGLPKDRLYVTVFREDDDAARLWEEVAHVPSGRIFRCDEK
                     DNFWAMGETGPCGPCSEIHYDFGPAGSEWGHADCPFPCECGRYVEIWNLVFMQFNRDA
                     AGVMTPLPSPSIDTGMGLERVAAILQGKLSNYETDLLRPIIDRAAQITGRTYGEDEKT
                     DVALRILADHSRATAFLIHDGVLPANEGRGYVLRKIMRRAMRHGRMIGMERPFLYELT
                     GFVAEIMKTGYPELLESVGRVARVVKEEEQRYAHTFVLAERVFEEEVQKISGCVIPGS
                     VSFKLYDTYGLALDEQEEMARERNLQIDREGFERAMSEQRARARASWKGAVKAAAAGV
                     YSQLHDLGPTKFEGYDSLTAAGCRVLALVAGEQIVDSVPEGARAGLILDRTPFYAEAG
                     GQVGDKGTIFREGVAVARVEDVVRPLPGLFVHKIAALAPLHTGDLMDCQVADRLRRAT
                     MRNHTATHLLHAALRQVLGPHVKQAGSVVEPGRLRFDFTHYAAMDRDEIDEVERLVNE
                     EILKNTGVVTDVMDLESAIATGAMALFGEKYGERVRVVQIPGFSKELCGGTHVRRTGD
                     IGLFKIVSESSISAGVRRIEAITGEAALEDYRRMTQAVERIAAAVRASEPELVESVER
                     LIAREREMEREMERLKSRIAQSQLGALVEQARTIKDVKVLAARVEGVDRTQMRSLADS
                     LRNKLKSCVVVLSSAENGNVAIVSAVTKDLAGRVHAGKIAGAVAEAVGGKGGGRPDMA
                     EAGGKDAAALPGALEKVYGLVESML"
     gene            19053..20279
                     /locus_tag="VFA54_17415"
     CDS             19053..20279
                     /locus_tag="VFA54_17415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZS52652.1"
                     /translation="MGHCEEKWRSGVSRRSALRGLAAFLAGSPLLRGQQDPFRDHSRV
                     PAMSELRTAFDFEPVAYAKLPRDIYDFMMGSSDDESTFRRNRRAFDWVTLVPRGVADV
                     SAINTATEIFGQKMAFPILVAPTSGQGPLHPEGEMGMHKGATAASNTTMIVSSGATFP
                     IEKIAEAGPGPLWFQLYRTDTVEGTIERVERAQAAGCTAIAWTVDGSVGPYRERVLHD
                     RHLGGYGAVDDNNPDSPRRRGRGAQVAQRRPNYGITAQELDWRFLETFRSSVKVPLLL
                     KGILTAEDARLAVEHGINGIIVSNHGARRVGHGCSSLEVLPEIVDEVRGRIPVLVDSG
                     FRRGSDVLKALALGAKAVCLGRVPRWGLAAYGPAGAQRILEIVQAELVLAMADTGRPS
                     LDAVDRTLVRTNFPWA"
     gene            20270..21355
                     /locus_tag="VFA54_17420"
     CDS             20270..21355
                     /locus_tag="VFA54_17420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HZS52653.1"
                     /translation="MGITRRGAVETLGLWLAGSPLLKAQDAAPKLIGEPPGRITPRNE
                     LVNTFEVEAMAQRSLPAAVFSKIAGSGNRDAFNRIIFLPRMMVDVRQLDLTLDLFGTK
                     MYAPILVGPASGQQRFHPDGELAVVRGASAAQAAVVISSRSSFPIEKIAAEASTTLWY
                     QVYPEPDMAPVLARIQQAVKAGCKAVCVTIGTPYEPAPAGAPPMQFRRLGSPRVDWAI
                     MDQVRRAANAPVLLKGVMSPEEARTAVERGASGIVVSEGGRFVHGLASPIEALPGIVD
                     AVGGKIPVLIDGGFRRGTDIIDALALGARAVLVARPALWGLAAYGAQGVQTVLEMLQS
                     ESARTMGLCGKPNLAALDRTLLRIDRR"
     gene            21686..>23589
                     /locus_tag="VFA54_17425"
     CDS             21686..>23589
                     /locus_tag="VFA54_17425"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS52654.1"
                     /translation="MRKQLSVVFVLSVAAALAVPSFAQTFGEITGVVTDPSGAVVVNA
                     TVTVTNPQTNFTRTALTNSAGNYTFPALLPGVYNVRAEMQGFQTEVRSGVELQVQQTA
                     RIDFQLKVGAVAEAVEVQGGAPLLATENATVGTVIDNQRIVDLPLNGRNFLQLVALSP
                     NVNATFADGGAATSRQGGDRASQNFSVAGQRREFNYYTLDGVANTDVDFNTYSFLPSI
                     DALQEFKVQTGVYSAEFGHEAAQVNVSTKSGTNEYHGALFDFIRNNDLDARPFGFTKT
                     VPVSAPFKWNQYGFTLGGPVQIPKVFNGKNRLFFMSNFEGFRLRNQAQVVYSTPPVAM
                     RNGDFSLAPVSQIIDPANNTPFPNKQIPSSRFDRAALGLLEFYPAPNIPGAGLANNYL
                     SLQNNVTDKDQFTQRIDFVESSRSSWFGRYSWQDDRQVQPALYLNGHTLAVTVKQAMI
                     SNTRIFSPSLVNEFRFGYNGFYNDYANELQFKRDPIKEFGIGLIDPAPEAWGTPGVSI
                     LGFSGFGDDVNGPFIVYDHTFQWIDNVSWTRGVHSLKFGAEIRRDRFNQTGNQNARGV
                     LTIDNPASGYGFADYMLGYVTRTQDAGSLAISQFRATSQAYYVADTWKARPNLTLDVG
                     LRYEYSPPWT"
BASE COUNT         4564 a         7037 c         7320 g         4668 t
ORIGIN      
        1 aaagatgaat attttttaat ttttctttca tcgacacttc atctgctctt gacagactgg
       61 ggcgtctgct acaaagcgga taattccgag gagagaaaca atgaagaaac tgttcagcat
      121 gctgctggtg ccgatcgttg gcgtcgcgct cacgttggcc gtgccgacaa tcaccaaggc
      181 ccaggatcag aaagacgaaa gctccgcacc gaagaagaag ggcaagaaga agaagaagga
      241 cgagaccaag aaggacaatc ttcgctaacc aaacagggcc ccgcaaggga ccattgctga
      301 aggagagagg gaatgaagcg gctgttcact gtgatgttgg tcccgattgt cggcgtggct
      361 ttgtcatttg ccgtaccgtc ggtctcaaag gcacaagaca acggtactac aaagaaagaa
      421 agcaagaaga agggcaagaa gaagaaaaag gacgactcgg ggaaggcgaa ctaagttcgc
      481 tttcgctcgg gcttagggct gcgggggacc gcgatcctac cttttcgcgg gtcccccttg
      541 cggcttacaa taaaagcagc taagtagctt ctgaccggcg tttccgagta tagctgtgcg
      601 tattctactg gtagaagacg agctaaaggt agcgagcttc atccgtagcg ctctcgaaga
      661 aaattcgtac gcagtggatg tcagtcatga tggggaagag ggatgctaca tggccgagac
      721 aaccgactac gacttggtca ttctcgacat catgctcccc aaaatggacg ggttccaaat
      781 gctccggcgg cttcgctcgc gcaaacctgg tctgccggta ctggtgctga ccgcgcgcgg
      841 caacattgaa gaccgcgtgc gcggcctgga tttaggagcc gatgattacc tggtaaagcc
      901 gtttgctctg gctgaactga ttgcccgcgt ccgcgcgctg ctgcgccgtg gggtccggga
      961 atcgaccacc gaactgcgcg tggatgacct agtcctcgat tcggcgcgcc gccgggtcac
     1021 tcgggcgggc aagccgatcg acctttccaa caaagaatac agcctcctcg aatacctgat
     1081 gcgcaacgcc aaccacgccg tcacccgcgg tatgatcgcg gagcacgttt gggattccag
     1141 cttcgacagc tttacgaacg tgattgacgt ctacatcaac catctgcgca ataaaatcga
     1201 ccggaatttc ggcaaaaagc tgattcacag cgtccgtgga gtcggctatc gagtttccgt
     1261 caacgactcg gagcaggaac cggtttcgta gtccttcttc tgggcttctc tttgcatcca
     1321 tctcgtgccc tgtacccaca ccgctcgtgc cggggcttgt aagctcattg tctgcaaatc
     1381 agggagaaaa cggctcaggg aagcggggcg gccgcgcgtg gccgtgccgg cccgttttcc
     1441 aagtgttcgg gccggacttt caatcgatgt aatcgtaagc gaccagggtg aggaattcgc
     1501 tgaagtcctt tcgcgtcatc atgtcttcga acaacttcga ggccagatcg aatttgccgg
     1561 aatcgaagcg cttggggcca atcagcttgc gaatgtgttc gagttgttcc gggatgacct
     1621 ttcggataag ctcggcgttc acggggcggc cgtcgttgag tttggcgccg tgccggaccc
     1681 attgccaaac ctgcgaacgg gaaatttccg ccgtggccgc gtcctccatc aggttataga
     1741 tcggcacgca gccgttgccc atcagccagg cctcgagata ttggatgccc acatcgatat
     1801 tcacgcgcag cccttcttcc gtaatgtcgc cggagggcac ggccagcagg tctttggcgg
     1861 agatgtgcac gtcttcccgc ttattgctga tctgatgggg agtcttcata tgttggtcga
     1921 agatctcttt ggcgacgccg accagccccg ggtgcgccac ccaagtgccg tcatgaccgg
     1981 ccttcacttc ccgcagcttg tcctgccgca ctttttccag cgctttctcg ttggcatcgg
     2041 ggtcgttttt gatgggaatc tgggccgcca tgccgcccat cgcgtggatc ccgcggcggt
     2101 ggcaggtttt gatcagcaac tggacgtagg cgttaaggaa ggccttgtcc atggtcacct
     2161 gcgcgcggtt cgggagaacg aactcaggct tattccggaa ttttttgatg aagctgaaaa
     2221 tgtaatccca tcggccgcag ttgaggcccg aggagtgatc ccggagctcg taaagaatct
     2281 cgtccatctc aaaggcggcc agaatcgtct caatcaggac cgtggcccgg atgctccccc
     2341 gcggaatccc gagcgcgtcc tgcgcgaaat tgaatacgtc gttccagagc cgggcttcca
     2401 ggtggctctc catcttcggc aaatagaggt acacgccggt gcctctcttc aaacgctctt
     2461 cggcgttgtg gaagaagaac aggccaaaat cgaacaggga tccagaaatc ggctggccgt
     2521 cgacgcgcag atgcttttcc accagatgcc agcctcgtgg gcggaccagc agggttgcca
     2581 gcttcggtcc tagtttgtag ctcttgcctt ccggactggt atagctgatg actccccgca
     2641 cggcatcccg cagattgaac tggccctcga tgttgttttg ccaggtcggc gagttggagt
     2701 cttcgaagtc ggccatgtag acattcgcac ccgagttgag cgcgttgatg atcatcttgc
     2761 ggtcaaccgg gccggtaatt tcgacacgcc ggtcttccag gtctttcggg atcggagcga
     2821 cagtccactc cttctcgcga atggattttg tttccggaag aaaatccggc attttgccgt
     2881 catcgatctc ctgttggcgc gcgacccgcc gctggagcag ctcctgccgg cggctttcga
     2941 attttctgtg gagtttcgag agaaacttca gcgcctccgg agtgaggatt tcggaactga
     3001 tgtcgctggc tttcccgagt acctcgatgc cttcttcccg cagcacttcc gtggatggtg
     3061 tagccatgat tgcctcctgg tcaagtcaaa aaaagttcgc cggaatctag taatcatacc
     3121 tcagccgcac gcacaaaacg aacatttccc aacgccgcgc tttcgccggc gctgtactcc
     3181 ggaaatcccg ccggttttca gagacttgcg gaaacgccgg ctgggcgggc gagagcgccc
     3241 ggccggccca tccgtttaac gtcagatctc gccatacaac atgaaaaagg ggccattgga
     3301 cggattctca tggcatggcc ggttccgctt gaataccatc cgaggagaag tcctgaagcg
     3361 gaaggtgaag tgtgtgtgta agttaggtcg ctgaaaggct agcgcccgac gagaggatta
     3421 ggagacgcgc cgaatgcata aaccgatcaa gtacctggag aagggtgtca gcattgccgc
     3481 cgctggaatg tggcacgctt tccgattcat caatgaccgt gttcccgaga gcccttcctt
     3541 cacgccccgg tggtccgata agccattgct gaagtcttac cagaaaacga agcctccact
     3601 cggctggccc cgcacgacgg actcgctgtg cccgcgctgt gtgccggaaa tccgcaaaga
     3661 gatcctggat ggtaagaaag attacaaaat tttgctcaat gagcgcgtcg gcgagctaaa
     3721 ggcgcaaatt atcgagcgcg atggcaagat cctgatggta aaggattgcc cgaagcacgg
     3781 gcatttcgaa gacgtcatgt cgattgacgt cgaatttttc aggcatctcg agtcggtttt
     3841 tcccggccgg gatattcgcg cccacaacga cgagaaactg cacaatcacg gctccagcac
     3901 catcaaacac gggcgcggat cggttctgac catcgacctg acgaaccgct gcaatatgat
     3961 gtgcgacccg tgctttatgg atgccaacca ggtgggcttt gtgcatgagc tgacttggga
     4021 cgacatcaag acgctgctcg acaatgcgat ttcaatcaag ccgcggcggc agatgtcggt
     4081 gcagttttcg ggcggcgagc ccaccatgtc gccttatttt ctggatgcgg tccgctatgc
     4141 caggaaagtt ggttacaaca gcgtgcaagc ggcgactaac ggcatcgagt tcgcgaagag
     4201 cccggagttc tgccgccagg cggcggaggc cgggctgcgc tatgcgtatc tccagtttga
     4261 cgggattggc aacgccgcca acgcccaccg caaggtcggc aatctgtttg acgtgaagat
     4321 gcgcgccatc gagaacctgc actccaacgg cgtcgaaatc gtgccggtca ccactttggt
     4381 aaacggcatc aacaacgaac aagtcggccg catcgtgagg ttcgcgctgg ataacccggg
     4441 caaaatcgct ttcctctcgt tccagccggt ctcctttacc ggaagagacg aagcggtgac
     4501 tgacgagcgc cgcatggccc agcggtacac gctgtcgcac ctcgcccacg acgtgaaaaa
     4561 ccagcttggc atcggcgagc cgaaacgcga ctggttcccg ctttctttca tgggcacatt
     4621 ctcggactgg tctgacttgg tgcacgggcc gggagccaac tggggccagg tgagctgcgg
     4681 ctgccatccg aactgcggcg tcggcatggc gattatggtc gataagcaca cgcgcgaggc
     4741 tgtgcctgtt accgcgtttc tgaacgccga ccagcttgcc aaagatgtgg cgaaggtgaa
     4801 cgattggggg cgcgggaagt ggctgtccat cgccggcatg acactggcct tgctgaaaaa
     4861 ttacgatccc ttccagacgc cgacgcattt ccggctgttt gatctgctga agaaactgga
     4921 caagagtctg ggcgcgaccg gctacaacta cggggatgtt tccggcaacc gcagcatgaa
     4981 agacattttc gagcggcgga aggatcggtg gaaccttttg ttcgtcgccg gcatgtggtt
     5041 ccaggatctg ttcaattacg atttccgccg caccgaacag tgcatcatcc cgtacgccac
     5101 gcaggaaggc gaaatttcct tctgtgccta caacacggga atcggctggc gcaatatcat
     5161 cgaaaagatg cacatgaccg ccacgctgac caagtggtac gaggagcacg ggcggcatga
     5221 aatcttcgcg ggcggcaaga acgtgaagct gaacagcacc gagcatcatc tggtgctccg
     5281 ggacgagatt gtcaccaagg aagaacagca cgatttggac aagcttggca tcgcgaagaa
     5341 cgcccgcgaa gagaaaatcc gcgcccgcga tgagagattg cggcagcagc aggaaaacgc
     5401 ccgtatggca aaactgtacc ggcagcacgt tttgaaagag ccgcccgccc ccgaactcgt
     5461 gcaattgcag ccgttgatcg catccgaggc caagccgcag gatcagcccg ctaacgaact
     5521 cgtcggaagc tgcggcgact gacgccgcat ctagaaccgg ttgctagtct ctcggcccgg
     5581 ctgctgtgac ctttcggtga cggcttctgg gccgcttgtt ttgccaggaa tgctccccgt
     5641 cggcgccgct ccgcctggag ccgcgtgcgg ggcgccatgc ttcggcagat cccacggcat
     5701 tgcaggacgc tcggacgaga cgctggattt ccattaaaag cgcggcgcaa aggcgaggga
     5761 gaacgggggc cgtgcgccgc tgctgggctg gcgccccatc cccaggttcc cgggtcaccg
     5821 ggatccgaga gacctcaaga attccccggc cagctcgtat tcatccgtcg ccacattgtc
     5881 gaggcccgcc tcccaggccg cgcgccagcg tgccagcagc gcctctcggc tgccaaaatt
     5941 atcttccggg ctccaggccg tgctatcggc gttcagagtc caggcgcgaa gccagtagcc
     6001 tttggagcgc cccgcttcca ccagttcgat cagccgattc cgttcctccg gggtgaaatc
     6061 cccagcctga ttgttgcggg atttctcgat gtgcgcccaa ttgaaattga atactcgata
     6121 gaacgcggtg gccggctcgg gaaagtagtt tgcaacgttt tcctggaatt tattgtcgtt
     6181 cggaggctct cgattcccca ttgccaggta gggctcggtc gggggagtaa gcctttcgaa
     6241 catcgcgatc gcctcctggt cgccgctcaa cagaacagta agaggacgcc aatcgagcgg
     6301 ggttgcaggc gagtcgcccc gtttgccgca ggcggttatc agagcccggc gccgttgcag
     6361 gaactggtac acctgtttta ccgtgccggg ctggtggtcc ttgaaatcga agattacgag
     6421 aattccgggc cggcctcgcg gcatcttctc cagctcgggc agcatcggtt tccagaagta
     6481 agcttcgatc gtgggctcgc tccccgtgag ttttgtcgta tgagtgatga cggcccggcg
     6541 gcggcgttcg gaccacgcca gatcgatctc gatcacggag agaccgctcc gccgggcccg
     6601 ctcgaggcgg tcggcgccgt ggtctccggc cggatagcaa ttgtgagcgt tcaaaaacca
     6661 ctggcgcgga taatcagccg ctctcaggct cgccgccgga agcaagcaaa gcaggcaggc
     6721 ccagaagaat gggaagcgcc gcgaggcgat aagaggcaag gtgattgacg aaggcatcat
     6781 tgccgctccg ccatctcggt cacccgaaga ccgttccccc aggggaccaa gcggaagccg
     6841 gtagtcccgc gtggttcgca agccggacct gccggtccag tctaaccagc gtcctgcgat
     6901 gtgctgttct ccgccccatc attttgtttg gcttacgcat aatagcatgc gccgccggga
     6961 aggcccgccg ggcgcccaaa agggctcggg acatggcttt ccctagggca agcccggcgg
     7021 ctctggggaa cggtgttgcg ggctccggac cgtcactttt ttggccttga cgcggctcgc
     7081 ggcgatgtgt aattattcat tggagtgaat attcattcat cgaggggaaa ttgcctgccg
     7141 ccacgttaac cagccagatc ccgaaggtct ccacggcgcg attcaccacc gaccttgatc
     7201 ttaccagcga cgaactggcc gccttgttcc tgctggcggc gcgcgtgaag cgctctccac
     7261 gccaattcgc gcacaagctc tccgggcgtt atttggcctt gctttttgag aagccgtctt
     7321 tacgcacgcg catgacgttc gagctggcga tgaagcagct cggcggggat gcggttttta
     7381 ccgattgcaa cggcggccgg attggcgatc gggagccgat tcaggatgtc gcgcgcaacc
     7441 tggagcgctg ggtaagcggc atcgtggcgc gcacgtacca gcaggccacg atcgacgagc
     7501 tctcgcggtg ggcggccgtg ccggtgatca acgccttgag cgacttatat catccctgcc
     7561 aggtgctggc cgactttttt accttgcaag agaaattcgg acaattgcgg ggtctgaagc
     7621 tggcgttcgt gggggacggc aacaatgtgg cccattccct gatgctcacc gggtcccggc
     7681 tgggcgtcga cattgcaatc gccacgccct cgggttacga gccgaatcgc gaaatctttg
     7741 ccatggcggc gggcttcgcg gcagcggcgg gctgctcctt gcggcttaca aacgatgcgt
     7801 ttgaggccgt ggagggcgcg cacgcggttt atacggatgt gtgggccagc atgggtcagg
     7861 aaaaagaaag cggacgccgc cggaaggatt ttgcgggcta tcaggtgacc ggcaagctga
     7921 tggcggcggc tctgcccgag gcggcattca tgcactgcct gcccgcgcat cggggcgagg
     7981 aagtggtcga tgaggtgatg gagtcggccg catcgatcgt cttcgagcag gccgaaaacc
     8041 gcctgcacgc acagaaagcc ctgctcttga tgatgctatc gtagaaacct gtggcccgca
     8101 tccggcctcg cgtcgaaagg cggctcggcc tggatgcgcg cgacaagcca acaggcgatt
     8161 gactcaatga ccaaaccaaa aaaagtggcc ctggcttatt ccgggggact ggacacctcc
     8221 gtcatcattc cctggcttcg cgagcattac gggtgcgagg ttatcgccgt ctgcggcaac
     8281 atcgggcagg gagaggaact cgatggaatc gtcgagaagg ccaaaaagag cggcgcggcg
     8341 gatgtcttcg tggaggatat gcgcgaagag ttcgttaccg aatatctctg gcggatggtt
     8401 cgcgccgggg cggtttacga gcataagtat ctgctaggca cgtcggtcgc gaggccgctt
     8461 ctcgccaaaa ggcaggtgga agtggctttg cggacaggct gcgacgcgct ggcccacggc
     8521 tgcacgggta agggcaatga tcaggtgcgc ttcgaactca cctataaagc tctggcgccg
     8581 cacctcgcgg tgattgctcc gtggcgcgaa tggacgatcc ggtcgcgcga agacgctctc
     8641 gactatgcgg caaagcataa tattccggtg gacgccagca cggccaaaat ctacagccgc
     8701 gaccgcaaca tctggcacat atctcacgag ggcggcgctt tggaagaccc ggccaacccg
     8761 gctccggatg acgtgtggat gcttacccgg catccctggg aagctcccga tcggccgggc
     8821 gcggtcacta ttggttttga gcgcgggact ccggtttcgg tggacggcgt aacaatgtcg
     8881 ggcgtcggga tcctcgagaa gctgaaccat cttggcgctg aacacggggt gggccgcatc
     8941 gacctggtgg agaaccgctt cgtggggatg aaatcccgcg ggtgttacga gacgcccggc
     9001 ggtacgctga tcctgctggc tcaccgcgaa ctggaagccc tcaccctgga tcgcagcacg
     9061 catcactaca agcagcggct cgcgctcgac tatgccgaga tggtttacaa cggcttttgg
     9121 ttcacgcccc tgcgcgaggc gctcgacgct ttcgtgtcca aaacgcagga aaccgtgacc
     9181 ggcgagatca agctgcggct cctgaaaggc aatgtggacg tgctctcgcg gcgctccccc
     9241 tactcgcttt attccctgga tatcgccagc ttcacgatgg gcgcctcgta tgatcagaag
     9301 gacgctctgg gcttcatcaa cttgatcggc ctgccgatga aagttcgcgc cggggtaatg
     9361 aaggcaagcc aatgaagctg tggggagggc gtttcgagaa aggcccatcg gcggcctttg
     9421 agcgattttc cggctccctc cgctacgact atcggctttg ggaagcagat attctggggt
     9481 cccagatttt cgcccgggag cttgagcggc aaggtattct cacggccgat gagcgcgaac
     9541 ggatttgccg ggcgtttgac gagatggccg cggagggacc aggggccgaa ctggctgccg
     9601 gagcggcgga cgaagacgtc cacacgctgg ttatccgcaa gctgaaagaa aaaattggag
     9661 aggccgccga caagatccac accgggcgca gccgcaacga gcaagtttcg ctggatttcc
     9721 gcctgtggat tcgcgatgaa atcaggacgc tgcgcggccg tctggcggac ttgatggaag
     9781 ctttactcgg gctggccgag cgcgacggca tggccattct acccgggtat acgcatctgc
     9841 gccgcgccca gcccgtgctg tggggccatt atctgctttc gtatttcgaa atgttagcac
     9901 gcgacgccgg gcgctttgag cacgcatacg ccgccgccga cctgctgccg ctaggctcgg
     9961 gggcgcttgc cggaagcggc tttccctttg accgggagcg catggcgcgc gagctcggct
    10021 tccgcggcgt cacgcggaac agcctggacg ccgcgggaga ccgcgatttc gccttggatt
    10081 tcctctacgc ggcggccgtc acgatgctgc acctgagccg gctggctgaa gattggatcc
    10141 tttactcgag cgaagaattc ggctttctgg agctcggcga cgaggtcacc agcgggtcca
    10201 gcttgatgcc gcagaaaaag aatccggatt cactcgagtt gattcggggc aaggcgggcc
    10261 gcgtggcggg gtcgctgctg tcgctgatga tgaccgtcaa gggcctgcca tctgcctata
    10321 atcgggactt acaagaggac aaggaagccg tcttcgacgc cgccgaacag ctcgcggcgg
    10381 ccgtggagat ggctgtggcc gtcgccggat cggttcgttt gaacaaagaa cgggccgaag
    10441 cagccgcgcg gcagagctgg actacggcga ccgagctggc agaggcgctg gcccgcaagg
    10501 gcgtcccatt tcaccgagcg catcagattg tcggcgggct ggtgctcgag agcctccgtt
    10561 tgggcagaca gcctgcggat tggacgcccc gacagcttgc ggatttcgcc gccgagctgg
    10621 acgctggaat ggctgccttg ctcgatccga ggcgggcggt tgaaaatcac gctctgccgg
    10681 gcgggacagc tcctggcgcc gtgcggcaag cgatggaaga agctcgccgg cggctcggcg
    10741 agatccgggc gaagtcgctg gctgtgggat aggcgacacg gcaaatacgt ttcagccaac
    10801 ggcctttccg ggatgtgcgg tgaaacggcg gccgcggagg cgctgcgccg cttctttgta
    10861 cttatgaata aaaagttccg ccaggggcag attcagaaaa tcattcgctc ccgcaatatt
    10921 catacgcagg aggagctggc cgaagcgctt caggcctcag gcattcatgc tacccaggtc
    10981 actctgtcgc gcgacatccg cgaactaggc attgtcaaga cccccgaagg gtaccgcgag
    11041 cgtcaggctc tggaaggcgc tcccgcggcg gatgaggcgt ggcgcgcggt ggaggagttc
    11101 ctgcgcgatg tgcgcctggc gaataacctc ctcgtactca agaccgatcc gggcaacgcg
    11161 aacccgctcg ccgtcgcgct cgatcgcgcg ccgtggccgg aaattgtggg cacgatcgcc
    11221 ggcgacgata ccgtgctggt gatttcaggc gataatcaat cggcgaggaa gttgcaagaa
    11281 cgtttgaccg ccgccctggc ggcgaccagc gccgccgcgg cggcccgcgc agggccgcgc
    11341 aaagaacgca aagcaaccgg aggcaaaccg tagagcggct gcgcaagcgc acttgctttc
    11401 cactgattat ttcagatcga tcgcgatcct ctccacggga tggtccagcg gattttgctc
    11461 ggtatgcgtc atcggtccat ccaggcgcac ctcgcccgcc ttgccccggg cctgatcgtt
    11521 gaggtagaca acgatgtgct ccaatgtgtg ttcgtgcaaa acgccctttt cgcggggacc
    11581 gaagcgaacc cgcagcacgc gtacctggtc gttttccaat tccagcgaat aatgtttcgg
    11641 atcaacgtgg agcggatcga tcttcgagac cggcgcaggc gaacctgacg gcttgccttt
    11701 cagctcgatt tccaccacct gaaacggctc cccgccgacg ttttcgctga tatgaggtcc
    11761 ggcggcggga ctccacagca cctgtccctt tttcaccggc atattctcga ccgtaccggc
    11821 cggctcagtg aatttcattt ggccgccgcc gaggtaaatc atgacccggt tcatggtgtg
    11881 ctcgtgcagg gcgctcggat gatgcggctg ttcggtggcg accagaaccc ggactttcgg
    11941 cgtgtcgatt ttcaccccgt ctggaacctg cgcctggagt ccagaaacca gcagcacagc
    12001 agccaatccc ccggccagcg ccaggctgga aatttttctc atgaatttcg ctcctcgaag
    12061 agtgggtcgc ctccgctaca agtcgactcg gcacatgtaa cccgcgctgg ggctgccgtt
    12121 cgagccgccg cccacgacaa ggccggcatc acagtagtag agatgcccct cgtggaagca
    12181 cattccgtga ggatcggggt cctgcccttt gacgagggtg accacttcaa gcacctttcc
    12241 cgtcgcggcg tcgaccttgt ggatcacgta atcactggaa aacagcaccc acatggcgcc
    12301 gttgtcccag tcgattccgt gagcccggtc gtgctggacc ggaatcactc gcagcacccg
    12361 aaaattatcc ttccaatcca gctcggccag cgccttgatg ccgagcgcgg tcacccaaag
    12421 cgtctgcgtc tgctctacat acgcgatgcc atgagaacct ccgggccacg gcggccggta
    12481 cctgcggaag gttttgccgg tttccaagtc cgcttgaaat atctcatcga aatcgttgtc
    12541 ggtcgggcga gggggcctgc gttccgttct gccattggcg gacatccaca gataaccgcc
    12601 tcccaccgcg attccgcttg tgttgtgcga atccgtctgc acctcgtgca gcaccctgcc
    12661 tgtcttccag tcgactttgt acgccttttc ggtcacctgg ccgccgatcc acagtgcgtc
    12721 gcgggtagcc tcgagcgcat tgggatgctc gtccggcgct ttgaacagcc tcaccacctt
    12781 tgccttccgg acgggtacgc cttgctccgg cttcgcgccc gcaggctcct gcgcgcgggc
    12841 cagcgctgcg gcgcccagcg ccccgcctag aaaacttctg cgtgtgatcg tcatacggtc
    12901 ctcgattgca aaatcataat acgcggcgcc gccgcggagc cgcggttctg ttcccgttcg
    12961 aatcctcgtt cgggtccaga acagagggag cacgcgccgg caaaaccgcg cggtaatgtt
    13021 ccgccggatg ggtctttccc atggtccgtc ccggggccag gcggcttata ccgccaaata
    13081 gggtttcagg gcttcgaagt tgtactcgat ttccgcaaca gcttccggcg tcagttggta
    13141 atcgccctgc cgtgtcacgc tggttttgaa gacgccgcgc ttcttcagga tataagggcg
    13201 cacgccggga attctctcgt cgagattcaa catcagcagg agcttgctga agacttcccg
    13261 ctgctcttgc agcttctgct gctgatgcaa gcgccagagc cgggcgaaca cgtcagcata
    13321 aggaacgccg ccgataatgg cgccgtcgga gcctaggcgc agctcgtaaa gcaggcctgc
    13381 gccccggttg gcacagaaca cccgcttaat cgggtcggga cggtgcgcga tttctgctct
    13441 catgcgggga atcacggggt cgtgctcctc tttaatgtag ccgaagttag gaaattccct
    13501 cgctaattcg acgatgaact cgaccgtagg ttcaacgtcg cgcgcgccgc ccgaggtttg
    13561 aatgaaaacg ggccgcttgg tggtcttaca caaggcccgg taataatcac gatagtcgtc
    13621 gagcgactgg gcggtggtgg gcggtatggc gatcacgccg tcaggctcca gcttttccgc
    13681 atgacgggca tattcgagca tttcttgtgt gtcgcgcccc tgcactccca gcacgagtgc
    13741 agggcttttg cctcgcgcgg ctttggccag cacctccatc ccgcgcattc gctcctcttt
    13801 cgtcagtttg gattgttcgc ttgcattttg gggccagact aaaccttgaa cgccgcaccg
    13861 gaccaggaaa tccacctcgc cggccaggtc ctcgtaatcg accgccttag ccggagtata
    13921 tggggtcgag agaatcatga aggcgccttg caggggtttg ccgggcgtgt tcccggcggg
    13981 ggtagcggtc aatcgggacg ccagacccgc cgtccccacg gttgccagcc attgccgccg
    14041 ggtatattcc gtcttcataa gccctcttgg cgcgaccgca tgaatcaggc tgcggcgctg
    14101 ctacgaccgc cggcctcgcc gtcatcgcag gagatcatat cgcggctgcc cgcccggcca
    14161 caagccgacg caggtacccc ttttatgtca ccgccgggct ggccggtgct tccagtggcc
    14221 tgatgctctg ccgggcgcct gcggctgcgt tacggtttcg gcgccggcgc cgagcctgtg
    14281 gcggggttgt aacgcgggct ggaggggctc gagggatcgg gcggggttcg cgccggccgg
    14341 gagctttcaa tccaggctgt gtagagcaaa tctcgcagca attggccggc ggcggccagt
    14401 tggctgttca ccagttcctt ggctgcttcg ttcgatttgc cggcgaacgc cccctgttta
    14461 tccagccggt agatttcttc gacgtgcgcc gccgaatggt ccaggtgctg taggatggcg
    14521 tcaaagggat cgggcagcaa cttcggcggt cccacgattg gcaaaaggtc ttcggcttct
    14581 actcccatca gatcgacaaa ggacgactca aagcgcgcat gaatgctgcc gtcccgggtg
    14641 tagccgtttg gattggggcc gcgccacccg tcgtggtgaa tggagtcgtg cattggctgc
    14701 gcgccgtccg caatgtagtg ccccagccat cctgtgtaga aagccagatc cagttccgta
    14761 tagcgcgtct cctgccccgc cgattgctgc gcccggtaga tcctcatgcc cgacttgagg
    14821 cgctcatagt tttccattgc ggcatagggc agggtccccg tccagcgcac attggtaagg
    14881 cgggcgcgcg ccggatcttt gtccttgatg cgaagatact cgtcatacaa agccagaacg
    14941 aactcgtagc gcgagcgggg aatctgcttc aggaacgcga actgttcctt gaaccatccg
    15001 tggttcggat cttcgatgat cttcagaaac ggctcggagg gctgccgcca ggtgtccggc
    15061 acccgtgcgc gcgcggcgat ccactcttcg tggacgcgta gaaaaatggg cccgtcctcg
    15121 ggcaggcttt gcaaggccag ccggtttacg acggcatggc cgcgcatgcc ccagccgtag
    15181 gcggtcccgg ccagcgcgag agccaaaacc ggcagatgtt ttattttcat gacgcttttc
    15241 cgctcccgat tatacgccgc cgggagcggc gagacgcagc ggctgcgagc tagaatcttt
    15301 ctgtgaaaac ccgcaagccc aagctgctca ccgagtccgg actcctcgaa tatgcgcttc
    15361 gcgcattgag cgcgcgcgcg ctcacgcagg gcgaactgcg ggtgcggttg caagcgcggg
    15421 ccgagcgacc gggcgacgtc gagaccgtgg tcggcagact caagcagtac ggctacctcg
    15481 acgaccggcg gttcgccgaa acattttcac ggctgcgccg cgaaaacgag ggtttcggca
    15541 aatttcgcgt gctgcgcgat ctgcgcagcc gccgcatcgc tccggggctt gccgaaaaga
    15601 ccgttgccga ggcgtatcgc gacgccgagg aagccgagct catcgaccgc ttcctcaagc
    15661 ggaaattgcg ctatagcgat cggcccccgg ccctgaacga tccacgccgc ctcgcctcgt
    15721 tgtaccgcat gcttttgcgg gcgggattca cgtctgctaa aattttggaa gcactccgca
    15781 aactgtcggt ccaatccgaa tgggtggaca gcctggaaac gacggcggaa gaagaggatt
    15841 ccgattgaca tcatatctct caggcgacca gattcgcgcc aaatttctcg ctttctttga
    15901 agcgcgcggc catcgtgtgg tgcggagctc gtccctggtg cccgccaatg atccgaccct
    15961 gctgttcacc aacgccggga tgaaccagtt caaagatgtt tttctggggc gggagaagcg
    16021 caactacacc cgggcggcca gcgcccagaa gtgcgttcgc gccggcggca agcacaatga
    16081 tctggagaac gtggggcgca cgcggcgcca tcacacgttt ttcgagatgc tcggaaattt
    16141 ctctttcggg gactatttca aacgggaagc catcgactac gcctggacgc tggtgaccgg
    16201 agattttggc cttcccaaag atcggcttta cgtgaccgtt ttccgggagg acgacgatgc
    16261 ggcgcgcctg tgggaagaag tggcgcacgt gccgtccggc cgtattttcc gctgcgatga
    16321 aaaggacaat ttctgggcca tgggcgaaac cggcccttgc ggcccctgtt cggaaattca
    16381 ctacgatttt gggcccgccg gcagcgaatg gggccacgcc gattgcccat ttccctgcga
    16441 atgcggccgc tacgtcgaga tctggaacct ggttttcatg cagttcaacc gcgacgcggc
    16501 cggcgttatg acgcccctgc cgagcccctc gatcgacacc ggtatgggtc tggaacgcgt
    16561 cgcagccatt ctgcaaggca agctaagcaa ctacgagacc gatttgctgc ggcccatcat
    16621 cgaccgcgcg gcccagatca ccggcaggac ttacggggaa gacgagaaga cggacgtcgc
    16681 cctgcgcatc ctcgccgacc acagccgcgc aaccgcgttc ctgatccacg acggcgtact
    16741 gcccgcgaac gaaggccgcg gctacgtgct gcggaagatt atgcgccgcg cgatgcggca
    16801 cggccggatg atcggcatgg aaaggccgtt tctctacgag ctgacaggtt ttgtggcgga
    16861 gatcatgaaa accggttatc cagaactgct tgagagcgtg ggccgcgtgg ctcgcgtggt
    16921 gaaggaggaa gaacagagat acgcccacac cttcgtgctg gccgagcgag ttttcgaaga
    16981 ggaagtgcaa aagatttctg gctgcgtgat ccctggcagc gtttccttca agctgtacga
    17041 cacctacggc ctggccctcg atgagcaaga agaaatggcc cgggagcgca atctccagat
    17101 cgaccgggaa ggcttcgaac gggcgatgtc cgaacagcgc gcgcgggccc gggcaagctg
    17161 gaagggggcg gtgaaggccg cggccgccgg ggtttactcc cagcttcacg atctcggccc
    17221 gacgaaattc gagggctacg acagtctgac cgcggccggc tgccgggttt tggccctggt
    17281 cgccggcgag caaatcgtcg attccgtgcc ggagggagcg cgggccggcc tgatcctcga
    17341 ccgcactccc ttctatgcgg aagcgggcgg gcaggtcggc gacaaaggca cgattttccg
    17401 cgagggcgtg gctgtggcgc gcgtcgagga tgtcgtgcgg ccgctgcccg gcctcttcgt
    17461 gcacaaaatt gcagcgctgg ccccgctgca caccggcgat ctgatggact gccaggtggc
    17521 tgaccggctc cgccgcgcaa cgatgcgcaa tcacaccgcg actcacctgc tgcacgccgc
    17581 gctccgccag gtgctcggcc cgcacgtgaa gcaggctggc agcgtcgtcg agccaggccg
    17641 gctgcgtttc gactttacgc attacgccgc tatggaccgc gatgaaatcg acgaggtaga
    17701 gcggctggtg aatgaggaga ttctgaagaa taccggcgtt gtaaccgacg ttatggacct
    17761 ggagagcgcg attgccaccg gcgccatggc gctgttcggg gagaaatacg gcgagcgagt
    17821 gcgcgttgtc cagatccccg gattcagcaa agagctgtgc ggcggcactc atgtccgccg
    17881 cacgggcgac atcgggctgt tcaagatcgt atcagaaagc agcatttccg cgggcgtgcg
    17941 gcgcatcgag gcgattacgg gagaagcggc gctcgaggat taccgccgca tgacgcaggc
    18001 ggtcgaacgc atcgccgccg ccgtccgcgc ctccgaaccg gagttggtgg aatcggtaga
    18061 gcggctgatc gcacgcgagc gcgaaatgga gcgcgaaatg gaacggctga agagccgtat
    18121 cgcgcagtcg cagcttggag ccctggtgga acaggcgcgc acgatcaagg atgtcaaggt
    18181 gctcgcggct cgtgtggagg gcgtagaccg cacgcaaatg cggtcgctcg ccgattcgtt
    18241 gcggaataaa ctgaaatcct gtgtagtagt gctgagttcg gccgaaaacg gcaacgtagc
    18301 catcgtttcc gcggtgacca aagaccttgc cgggcgcgtc catgcgggca agatcgccgg
    18361 agccgtggcg gaggccgtgg gcggcaaggg cggcgggcgt cccgatatgg ccgaggccgg
    18421 cggcaaagac gcagcggctc ttcccggcgc gctggagaaa gtttacggcc tggtcgagtc
    18481 gatgctctaa gtccccaggc ttggttggaa ctggctgttc agcgatagcg ccgccggatc
    18541 cttcgggccc actgatcgcc acaacgccgg cagggccaaa cgggtacaca tcttccggcc
    18601 attctgcggc aattgatttt cgcgatcccg tctctatgcc gccccggaat ggcaaagcag
    18661 gcctcttcgg ctgaagccga aagaaaacac gttaccttat tgcaaaaaat ccttggtagc
    18721 agccgaaaac gggactttcc atccggcaga cattaggagg tttcgaggaa ctttcgattt
    18781 cgctctgaca cctgtgccat cccgcgctgc ccacgcccgc gcttgacaac acctctgcgc
    18841 agttatacca ttccagcaac cttggcttag aagggggtag ccatgaaggc tggttcctct
    18901 ttggtggtgg cgcagtgcga tacagttttg cctcgctgcg ccgcattgcc gcccttctca
    18961 cggaccattt ccaacttaat ttcgttgacc gacaacgctt tcggaaagcg gcgcatcgct
    19021 ttccaagcgc acttccagtt ctaggagcgg ttatgggtca ttgcgaagaa aaatggcgtt
    19081 ccggagtctc ccggcggtcc gctttgcgag gtttggcggc cttcctcgcc gggtcgccgc
    19141 tgctgcgcgg ccagcaggat ccctttcgcg atcacagccg ggtcccagcc atgagcgaac
    19201 tgcggacggc ctttgacttc gaaccggtcg cctacgccaa gctcccgcgg gatatttacg
    19261 acttcatgat ggggtccagc gacgatgagt ccacgttccg gcgcaaccgc cgcgcgtttg
    19321 actgggtgac gctggttccg cggggggttg cggacgtcag cgccatcaat accgccaccg
    19381 agattttcgg gcagaagatg gcatttccga tccttgtggc tccgacctcc ggccaaggtc
    19441 ccttgcaccc ggaaggggag atgggaatgc acaagggcgc gaccgccgct tccaacacca
    19501 ccatgatcgt gagcagcggc gcgacgtttc cgattgagaa aatcgccgaa gctggtcccg
    19561 gcccactttg gttccagctt tatcgcaccg acacggtgga aggcactatc gaacgcgtgg
    19621 aacgggcgca agcggccgga tgtacggcga tagcctggac ggtagacggt tcggtcggcc
    19681 cctatcggga acgagtgttg cacgatcgcc atttgggcgg ctatggggcg gtcgatgaca
    19741 acaatcccga ttccccccgc cgccgcggcc gcggcgcgca ggtcgcgcag cgccggccca
    19801 attacggcat cacggcgcag gaattggact ggcggttcct ggaaaccttc cggtcttcgg
    19861 tcaaagtgcc cttgttgttg aaaggtattc tgacggccga ggatgcgcga ctggcggtcg
    19921 agcacggcat caatggaatc attgtttcca atcacggcgc gcggcgcgtg ggccatggat
    19981 gctccagtct ggaagtgctc ccggaaattg tggacgaagt cagggggcgc atcccggtgc
    20041 ttgtcgatag cggtttccgg aggggaagcg acgtgctgaa ggcgctggcc ctcggcgcca
    20101 aggccgtctg cctgggccgc gttcctcgct gggggctggc cgcgtatggc ccggcgggcg
    20161 cgcagaggat tctggaaatt gtgcaggctg agctggtatt ggcgatggcc gatactggcc
    20221 gtcccagcct ggacgcggtc gatcgcactt tggtgaggac gaacttccca tgggcataac
    20281 acgacgcggg gctgtcgaaa ccctcggtct ctggctggcc ggatcgccgc tgttgaaggc
    20341 gcaggacgcg gctcccaagc tgatcggcga gccgccgggc cggatcacgc cacgcaacga
    20401 gctggtcaac acgttcgagg tcgaagcgat ggcgcaacgg tccctgccgg ctgcggtgtt
    20461 ctccaagatt gcgggtagcg gcaaccggga tgccttcaat cgcataatct ttctgccgcg
    20521 gatgatggta gacgtccgcc aacttgatct gactctcgat ctgttcggga caaagatgta
    20581 cgcgccgatc ctggtggggc cggcttccgg ccagcagcgg tttcatcccg atggggaatt
    20641 ggccgtcgtc cgcggcgcct cggcggcgca ggcggccgtg gtgatcagca gccggtccag
    20701 cttcccgata gaaaagatcg ccgccgaggc cagcactact ctgtggtatc aggtgtaccc
    20761 ggagcctgac atggcgccag tgctcgcgcg tatccagcag gccgttaagg cggggtgcaa
    20821 ggccgtgtgc gtgacgatcg gcacccccta cgagccggcg cccgccggtg cgccgccgat
    20881 gcaattcagg cgtttgggga gcccccgggt tgattgggca atcatggatc aggtgcggcg
    20941 cgcggctaat gcgcctgtct tgctcaaggg cgtaatgagc ccggaagagg cccgcacggc
    21001 ggttgaacga ggagccagcg gcatcgtggt ctccgagggg gggcgttttg tacacgggct
    21061 cgccagtccc atagaagcgc tgccgggaat cgtggacgcc gttggaggga aaattcctgt
    21121 tcttatcgac ggcggtttcc gccggggtac cgacattatc gacgccctgg ctttaggagc
    21181 gcgcgctgtg ctggtcgccc gcccggcgct ttgggggttg gcggcctatg gagcgcaggg
    21241 ggttcaaacg gtgctcgaga tgctgcaaag cgagtcggcc cgcaccatgg gtttgtgcgg
    21301 aaagccgaat ctggccgctc tggatcgcac gctgttgcga atcgaccggc gctgacgttt
    21361 tggcagcaca ccgattcgaa gccaaagcgg cattgtctat cgcggcaggg gcattcggaa
    21421 gcgcgaaaaa agcgatcggg ttagcgggct aaccaggagg ctgcgccata gccacagcgg
    21481 cagaggtccc cgcgatgatc ttactttggt ctaacagcga ttctaacccc gccagccctt
    21541 cggcgggcgc gtggcctgtc caccggcttt ctatccgctt gaacgcggcg aatttcctcg
    21601 ttgattgctc cggcattttc atcgtttcgg gtctgagtgg gcctgtgccc aacgcccttc
    21661 gatccaagca aggggggttg cacgtatgcg caagcaactg agcgtagtct ttgttttgtc
    21721 cgttgcagcc gcgttggctg tgccatcgtt cgcccagacg ttcggggaaa tcaccggtgt
    21781 agttacggac ccgagcggag ccgtcgtggt caatgcgacc gtcacggtta caaacccgca
    21841 gacgaatttc acgcgcacag ccctgacgaa tagcgcgggc aactacacgt ttccggcgct
    21901 tctgccgggt gtttataacg taagggctga aatgcagggg ttccagaccg aggtccgcag
    21961 cggcgtcgag ctccaagtgc agcaaacggc gcgcatcgac ttccagctca aggtcggcgc
    22021 cgtggcggag gctgtggaag tgcaaggcgg cgctcctttg ctcgccacag agaacgccac
    22081 cgtgggcacg gtgatcgaca atcagcgcat tgtcgacctg ccgctcaacg gtcgcaattt
    22141 tctccagctt gtggctttga gcccgaatgt aaacgcgacc ttcgccgacg gtggcgccgc
    22201 tacaagccgc caaggcggcg accgcgcctc acaaaacttt tcggtcgccg gccaacggcg
    22261 tgagttcaat tattacacgc tcgacggcgt cgccaacacc gacgtggatt tcaatactta
    22321 ttcgttcctg ccctccatcg acgccttgca ggaattcaaa gtgcagactg gcgtttactc
    22381 cgctgagttt ggccacgaag ccgcccaggt caatgtttcg accaagagcg gaaccaacga
    22441 gtatcacggc gcccttttcg atttcattcg gaacaacgac ctcgacgccc gcccttttgg
    22501 tttcaccaag acggtgcctg tttccgcgcc tttcaagtgg aaccaatacg gattcacgct
    22561 aggtgggccg gtgcagattc ccaaggtgtt caacggcaag aaccgccttt tcttcatgtc
    22621 caactttgaa ggtttccggc tgcgcaacca ggcccaggtc gtctacagca cgccgccggt
    22681 tgcgatgcgc aacggcgact tctcgctggc gcccgtttct caaatcatcg acccggcaaa
    22741 caatacgcct tttcccaaca agcaaattcc cagctcgcgc ttcgatcggg cggccctggg
    22801 gctgctcgaa ttctatccgg cgcccaacat ccccggagcc ggcctggcga ataactactt
    22861 gtctctgcaa aacaacgtga ccgacaaaga ccagttcacc cagcgcatcg attttgtgga
    22921 gagctccagg tcgagctggt tcggccgtta tagctggcag gacgaccgac aggtgcagcc
    22981 ggcgctgtat ctgaacggcc ataccctggc ggtcaccgtg aaacaggcga tgatctcgaa
    23041 tacgcgtatt ttctcgccca gcttggtcaa cgaattccgc ttcggctaca acggttttta
    23101 taacgactat gccaacgaat tgcagttcaa gcgcgacccg attaaagagt tcgggatagg
    23161 gctcatcgac ccggcgccgg aagcctgggg cacgcccgga gtgagcatct tgggattcag
    23221 cggctttgga gacgacgtca acggcccctt catcgtttat gaccacacct tccagtggat
    23281 cgacaacgtt tcctggactc gcggggtcca ctctctcaaa tttggagctg aaatccgccg
    23341 cgaccgcttt aatcagacgg gcaaccagaa tgcccgcggg gttctgacga tcgacaaccc
    23401 ggcgagcggt tatggcttcg ccgactacat gctcggctac gtcacgcgaa cccaggatgc
    23461 cggcagtctg gccatcagcc agttccgcgc cactagccag gcctactatg tcgcagatac
    23521 ctggaaggcg cgccctaatc tgacgttgga tgtggggctg cgttacgaat actccccgcc
    23581 ttggaccga
//
LOCUS       DASREK010000318         7016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1314842, whole genome shotgun sequence.
ACCESSION   DASREK010000318 DASREK010000000
VERSION     DASREK010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314842"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VFA54_17430"
     CDS             complement(<1..704)
                     /locus_tag="VFA54_17430"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZS52655.1"
                     /translation="MSDQAFRIEKDSMGEVKVPADALYAAQTQRAVENFPISGLRFPR
                     AFIRALGMIKGAAAAVNLDLKLMDADMAKAIQEAAREVEEGLHDAHFPLDIYQTGSGT
                     STNMNANEVLATLASRKLGRKVHPNDHVNMGQSSNDVIPTAIHVSAYLEVHTRLLPAL
                     VHLRETIDRRARETDYIVKTGRTHLMDALPVRLGQELSGWSSQIAHAIARIESTLPRL
                     AELAQGGTAVGTGVNA"
     gene            798..1973
                     /locus_tag="VFA54_17435"
     CDS             798..1973
                     /locus_tag="VFA54_17435"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HZS52656.1"
                     /translation="MKSMFPRVIWIVLDSVGAGEMPDADRYGDRGSDTLGNIARRRPM
                     RLPNLRRLGLANIKPLENLPPAESPAGAYGRAALLSPGKDTTTGHWEMAGIILEKPLP
                     LFPRGFPRDFMDEFERRIGRGTLGNCAASGTEIIQRLGAEHMRTGSPIIYTSADSVFQ
                     VAAHEEVIPLAELYRICEIAREMLRGPLEVGRVIARPFLGSPGSFTRTANRRDYAIPP
                     PAGMLLDKLTEAGVPVHAVGKIADVFNHRGITSFVKTKSNDDGTSRTIEAAKQRDRGL
                     IFTNLVDFDMLYGHRNDVEGYAAALESFDQRLPELESILRPGDLVLLTADHGCDPTTP
                     STDHSREYVPVLAFGPRVRPGVNLGTRATLADLGQTVAANFGARIAVGTSFLQEIAT"
     gene            1970..2725
                     /gene="tpiA"
                     /locus_tag="VFA54_17440"
     CDS             1970..2725
                     /gene="tpiA"
                     /locus_tag="VFA54_17440"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZS52657.1"
                     /translation="MSTRVPVIAGNWKMYKTPGETVSFFEQFRPLVERSTHCEVIIAP
                     PFPCLPAAAEGARGSRIHIAAQNLHWEKEGAYTGEVSAGMLAAAGCTYVIVGHSERRQ
                     YFGETDQTVNRRVRAALGAGLQPIVCVGERLEQREAGETDSVLIQQFEGAFAGLTPEQ
                     FSPILVAYEPVWAIGTGRTATPEIAAQAHRVIRGRAARCFGAAAAESLRILYGGSVKP
                     DNIKGLMDRPDIDGALVGGASLDAASMASIVNY"
     gene            2750..3169
                     /gene="secG"
                     /locus_tag="VFA54_17445"
     CDS             2750..3169
                     /gene="secG"
                     /locus_tag="VFA54_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZS52658.1"
                     /translation="MHLLISILHILVCIFLIIVVLLQSGKAADLAGAFGGMGSQTAFG
                     PRGAATVLSKATTAAAVVFMITSIILSIMATKQGGVGGSVLERNTGAAASRPASGKTA
                     PVQVPVMPGQPITIPVPQQGGNAPAQGQPAPAAPAKK"
     gene            3204..3290
                     /locus_tag="VFA54_17450"
     tRNA            3204..3290
                     /locus_tag="VFA54_17450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3238..3240,aa:Leu,seq:gag)
     gene            complement(3406..5316)
                     /locus_tag="VFA54_17455"
     CDS             complement(3406..5316)
                     /locus_tag="VFA54_17455"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZS52659.1"
                     /translation="MAQPKMEAVRCLPMVVRLSNYTLGLITALVGLGIVRGWWTVALI
                     GLVIFVVVLYSSELHAAKLEAERRHAKEIAGLHLRTIEALAVAIDAKDNTTHQHLERV
                     VIYAEAIGKELNLPENELKALRTAALLHDIGKLAVPEYIISKPGRLTEEEFEKMKIHT
                     VVGAEILERVQFPYPVVPIVRSHHERWNGSGYPAGLKGEEIPIGARILAVVDSFDALS
                     SDRQYRRALSREEAMAALKKDAGTLYDPTIVEILEKIYRKIEPFPEPPAKERHKTTPP
                     VKIDRTCVPSSGFSVEKVGDTSSALYSIAQAREELQILYELTQALGNSLSVDETLRLV
                     AEELQKLIRFDAIAVYLIREGKLIPRFVEGVNASLLARMQIPLGEGLSGWVAANGKCS
                     LNGNPAVEPGYTQDPHQFSRLRCALSVPLDSPKGILGALTLYSTQHDAAFTVDHLRIL
                     KAVGMKTGSTLHNALIHEAMGRTAETDGLTGLPNARALSARLEQESARAHRQHTPFTV
                     FVIDMNGLKQINDTLGHLMGNKVIKAVAGALSSCVREYDLVARMGGDEFVLLLPEVDG
                     EAAATKRIELAMAVEALKKDFPQVEVSVSMGSASFPEDSRDVEKLMAIADRRMYWHKR
                     QHHRARAPISTA"
     gene            5577..6494
                     /locus_tag="VFA54_17460"
     CDS             5577..6494
                     /locus_tag="VFA54_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HZS52660.1"
                     /translation="MPELPDVVLYVEALEKRIVNQELRAIRVTSPFLVRTADPPIQAA
                     VGKRVRELRRLGKRIAIGFEADLWLVVHLMIAGRLHWREASSPAASRAPGGKQALAAF
                     DFASGCLLLTEAGSKKRAALHAVQGEAGLRELDAGGADVLAISLDQFGAALTAGNHTL
                     KRALTDPRLFSGIGNAYSDEILHRARLSPVALTSKLTPAQISALYDAARETLSAWIAR
                     LRAEAGGCFPEKVTAFRDGMAVHGRYRQPCPECGTAVQRIRYAENETNYCPSCQTGGK
                     LLADRALSRLLRQDWPRTVEELETARTRR"
     gene            6491..>7016
                     /locus_tag="VFA54_17465"
     CDS             6491..>7016
                     /locus_tag="VFA54_17465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS52661.1"
                     /translation="MTVIKEVPARDSALERSSKLPGRIDALLWTLLAVCIVRLWLAPL
                     GSSFWVDEMVTVFVVRHGAAHPSLAAAPQVGRSVYYFLPKAAEALLGFSEISYRVPSI
                     LAMGAAVFLISRLAARLIHPQAQWFAAFACLALPGLNYEAADARPYALGICAASASFF
                     FLVRWLDFARRRDAF"
BASE COUNT         1242 a         2146 c         2244 g         1384 t
ORIGIN      
        1 tgcgcgttga cgccggtgcc gacggccgtt cccccttgcg ccagttcggc caggcgcggc
       61 agcgtggact caatgcgggc gatggcgtgg gcgatctgtg agctccaccc gctcaattcc
      121 tggcccaggc ggaccggcaa agcgtccatc aaatgggtgc gcccggtttt gacaatgtaa
      181 tccgtctcgc gggcgcggcg gtcaatggtt tcccgcagat gaaccagcgc gggcagcagc
      241 cgcgtatgca cttcgaggta ggcgctcacg tgaatggccg tagggatcac gtcgttcgac
      301 gactggccca tattcacgtg gtcgttggga tgcaccttcc ggccgagctt gcgcgaggcg
      361 agcgtggcca gcacctcatt ggcgttcata ttcgtgctgg tccccgagcc ggtctgataa
      421 atatcaagcg gaaagtgagc gtcgtggagt ccttcctcga cctcccgggc agcttcctgg
      481 atggcttttg ccatatcggc atccatcagc ttcaaatcca gattgacagc cgccgcggcc
      541 cccttgatca tgcccaaagc gcggatgaag gcgcggggaa aacgcaaccc gctgatggga
      601 aaattctcga cagcccgctg cgtctgagcg gcatagaggg cgtcggctgg aaccttcacc
      661 tcgcccatgc tgtccttctc gatgcgaaac gcctggtcac tcatgaatac accggtcctt
      721 tccagtatta gcggtcagta ttgccggccc ggggctgcga ggcccgcaaa ttttgatgat
      781 aacagccggt tgctagaatg aaatccatgt ttccccgcgt gatctggatc gtgctcgaca
      841 gcgtgggcgc cggcgaaatg cccgatgcgg accgctacgg cgaccggggc agcgacacgc
      901 tgggaaatat cgcccggcgg cggccgatgc gcctgcccaa cctgcgccgc ctgggtcttg
      961 ccaatatcaa gccgctggag aatttgccgc cggccgagag cccggcggga gcttacgggc
     1021 gcgccgcgct cctctctccg ggcaaggaca ctacgactgg gcattgggaa atggccggca
     1081 tcattctgga gaagccgctg ccgctgttcc cgcgcggctt cccgcgcgat ttcatggacg
     1141 agttcgagcg gcggatcggc cgcggaacgc ttggcaactg cgccgcttcc ggtacggaaa
     1201 tcattcagcg gctcggcgcg gagcatatgc ggacggggtc gccgatcatt tatacctcgg
     1261 cagacagcgt gttccaggtg gcggcgcatg aagaagtgat cccgctggcc gagttgtatc
     1321 gcatttgcga aatcgcccgg gagatgctgc gggggcccct cgaagtaggg cgcgtgatcg
     1381 cccgcccgtt tctgggctcg ccggggagtt ttactcgcac cgcgaaccgg cgcgactatg
     1441 cgatcccgcc gcccgcgggc atgctgctcg acaagcttac cgaagcgggc gtgccggtgc
     1501 acgcggtggg aaaaatcgcc gacgtcttca accatcgcgg gatcacgagc ttcgtgaaaa
     1561 cgaaaagcaa cgacgacgga accagccgga cgatcgaagc ggccaagcag cgcgatcgcg
     1621 gtctgatctt taccaatctg gtcgatttcg atatgcttta cggtcaccgc aacgacgtcg
     1681 aaggctacgc cgcggccctg gagagcttcg accagcggct tccggaactc gagagcatac
     1741 tgcgtcccgg cgatttggtt ctcttgacgg cggatcacgg gtgcgacccg acgacgccgt
     1801 ccaccgacca ttcccgggag tacgttccgg tgctcgcctt cgggccgcgc gtgcggcccg
     1861 gagtgaacct gggcacgcgc gccacgctgg ccgatctcgg ccagactgtg gccgccaatt
     1921 tcggcgcccg aatcgcggtg gggaccagct ttttacagga gatcgccacg tgagcacgcg
     1981 cgtgccggtg atagccggca actggaagat gtacaagacg ccgggtgaga cggtgagttt
     2041 ctttgagcag ttccggcctc tggtagagcg ttcgacgcat tgtgaggtta tcattgcgcc
     2101 cccgtttccc tgcctgccgg cggccgccga aggagcgcgg ggatcgagga ttcacattgc
     2161 ggcgcagaac ttgcattggg agaaggaagg ggcctacacg ggcgaggttt cggcgggcat
     2221 gctcgccgcc gctggctgta cgtatgtcat cgtggggcac agcgagcgcc gccagtactt
     2281 cggcgagacc gaccagaccg tgaaccggcg cgttcgcgcc gcgctcggag cgggtttgca
     2341 gccgatcgta tgcgtggggg agcgactcga gcagcgggaa gccggggaaa ccgattccgt
     2401 tttaatccag caatttgagg gagctttcgc cggtttgacc ccggaacagt tctctcctat
     2461 actggtggct tacgagcctg tgtgggcgat tggaaccggc cgtacggcca cgccggaaat
     2521 cgcagcgcaa gcgcaccggg taattcgcgg gcgggcggcc cgatgctttg gcgcagcagc
     2581 ggccgaaagt ttgcgcatcc tctacggcgg cagcgtcaag cccgataaca tcaagggctt
     2641 gatggaccgg cccgacattg acggcgctct ggtgggcggg gccagtctcg acgcggcttc
     2701 gatggcctcg atcgtgaatt actagcgggc tcggaaagtc agcaatcgca tgcatcttct
     2761 gatttccatt ttgcacattt tggtgtgcat atttttgatc atcgtcgtgc tgctccagag
     2821 cggcaaagcc gcggatttgg ctggggcgtt cggtggaatg gggtcgcaaa cggcgtttgg
     2881 gcccaggggt gcggccactg tactttccaa ggcgacgacc gccgcggcgg tggtattcat
     2941 gatcacgtcg atcattctct cgatcatggc caccaagcag ggcggcgtcg gcggctcggt
     3001 cctcgagagg aacaccggcg cggcggcaag ccggcccgct tcggggaaga ccgcgcccgt
     3061 tcaagttccg gtgatgccgg ggcagcccat tacgattccc gtgccccagc agggcggcaa
     3121 tgcgccggcg caaggccagc cggcgccggc ggctccggcg aagaagtgag cccgcctgaa
     3181 gacaggagct tcccttcccc tgtgcggagg tggcggaatt ggcagacgca ctagcttgag
     3241 gtgctagcgg gagcaatccc atgggggttc aagtccccct ctccgcacca gatttcccgg
     3301 ctgttgctcc atgggtgaag atcgggcgcc ggggggcgtc gaggatttat tgccgggttg
     3361 tgtggcccgc tcggtttgtt cgccgcacct cggctcttcc gagcctcacg ccgttgaaat
     3421 gggagcgcgg gcgcggtggt gctgccgttt gtgccaatac atacggcggt cggcaatggc
     3481 catcagcttt tcaacatcgc ggctgtcttc cgggaaggaa gccgagccca tgctgaccga
     3541 gacttccacc tgcggaaaat ccttcttcaa cgcttccacg gccatagcca gctcaatccg
     3601 cttcgtagcc gccgcctcgc catccacctc cggcaacagc aggacgaatt catccccgcc
     3661 catccgggcg accaggtcat attcgcggac gcagctcgac agcgcgcctg ccaccgcctt
     3721 gatcactttg ttgcccatca aatggccgag cgtgtcattg atctgcttca ggccgttcat
     3781 gtcgataacg aataccgtga aaggcgtgtg ctggcggtgc gcccgggccg attcctgttc
     3841 gaggcgcgcc gaaagagcgc gggcgttggg cagcccggtg agaccgtcgg tttcagccgt
     3901 tcttcccata gcctcatgga tcagcgcatt atgcagcgtg gagccggtct tcatgcccac
     3961 ggctttgagg attcgcaggt ggtctacggt gaacgcggcg tcgtgctgcg tgctgtagag
     4021 cgtgagcgcc cccaggatgc cctttggaga atcgaggggg accgagagcg cacatcgcag
     4081 ccggctgaac tgatgcgggt cctgtgtgta gcccggctcg acggctggat ttccgttcag
     4141 gctgcatttt ccattggccg ccacccagcc tgagagacct tcccccagtg gaatctgcat
     4201 ccgggccagc aaggaggcgt tcactccttc cacaaagcga ggaatcagct tgccttcccg
     4261 aatcagatac acggcgatcg catcgaagcg gatcagcttc tgaagctcct cggccaccag
     4321 ccgcagcgtc tcgtccacac tcaggctgtt gcccagagct tgcgtgagct cgtaaaggat
     4381 ctgcaattct tcccgcgcct gcgcgatcga gtaaagtgcg gacgaggtat ccccgacttt
     4441 ctccaccgag aaaccggagc tcggcacgca ggttctatcg attttgacgg gaggggtcgt
     4501 tttgtggcgc tctttggccg gcggctccgg aaaaggctcg attttgcgat aaatcttttc
     4561 taggatctcg acaattgtcg gatcgtacag cgtgccggcg tctttcttca gcgccgccat
     4621 agcttcctcg cgagacagcg cgcgccggta ctgccggtcg gaagagagag catcgaagga
     4681 atccacgaca gccagaatcc gggcgccgat gggaatctct tcgcccttca aaccggccgg
     4741 atatccagat ccgttccacc gctcgtggtg cgagcggaca atcgggacca ccggataagg
     4801 gaactggacg cgctccagaa tttccgcccc gacgacggta tggatcttca ttttttcgaa
     4861 ctcttcttcg gtcaatcgcc cgggcttgga aataatatat tccggcaccg ccagcttgcc
     4921 gatatcgtgc agcaaggcgg cggtccgcag cgccttgagc tcgttttccg gcaagttcag
     4981 ttctttaccg atcgcttcgg catagataac caccctctcg agatgctgat gggttgtatt
     5041 gtctttggcg tcgatagcca cggccagagc ctcgatcgtc cgcaggtgca aaccggcaat
     5101 ttccttggca tggcgccttt ccgcttccag cttggcggcg tgcagctccg acgaatacag
     5161 cacaacgaca aagatcacca agccgatcag cgcgacagtc caccaccccc gcacgatacc
     5221 aagccctacc agcgcggtaa tgagtccgag tgtgtagttg gaaagccgga caaccatcgg
     5281 caggcaccta acggcttcca ttttcggctg cgccatcgtg ctatgccaat ggagcgctgg
     5341 tacttaattg gattggtgat atcccctagg ctgccgcgag catcgggcta tgctggttga
     5401 atttttcgag acagcgtatg cgccgcggcg attcgcaaat gctcactgca aaagcgaaac
     5461 tgagattttt tcgagagtgg gcgccagccc gggcgcagac cgctatagat gcctaacctc
     5521 acggaatccg tacaggccgc gttgccgccg accggcgcgt atggcaagct gttggcgtgc
     5581 cggagctgcc ggacgtcgtg ctttacgtcg aagccctcga aaagcggatc gtgaaccagg
     5641 aattgcgtgc catccgggtg acgagcccct ttctggtgcg caccgccgat cccccgattc
     5701 aggcggcagt cgggaaacgg gtgcgcgagc ttcgccgcct ggggaaacga atcgccatcg
     5761 gattcgaggc ggatttatgg ctggtcgtcc atctgatgat cgccggacgg ctgcactggc
     5821 gcgaggcttc gagtcctgcc gcgagccgcg ctcccggcgg gaagcaggcg ttggctgcct
     5881 tcgatttcgc ttccggctgt ttgctgctca ccgaagcggg atcgaagaaa cgggcggcgc
     5941 tgcacgccgt ccaaggtgaa gctggcttgc gcgagctgga cgcgggaggg gcggatgtgc
     6001 tcgcgatatc tttggatcag tttggcgcgg ccctcaccgc cggcaatcac acgctgaagc
     6061 gagcgctcac ggacccgcgc ctgttcagcg gcataggcaa cgcttattcg gacgaaatct
     6121 tgcaccgagc gcggctttct cccgtggcgc tcacctcgaa gctgacgcca gcgcagatct
     6181 cggccttgta tgacgcggct cgggagacgc tctccgcgtg gatcgcgagg ctgcgggccg
     6241 aagcgggtgg atgtttcccc gagaaggtca ccgcgttccg ggacggcatg gccgtgcacg
     6301 gccgttaccg ccagccctgc cccgaatgcg gcacggcggt gcaacggatt cgctatgcgg
     6361 aaaacgagac caactattgc cctagttgcc agacgggagg caaactgctg gcagaccggg
     6421 cgctttcccg gcttctaaga caagattggc cccggaccgt ggaggagctg gaaacggcaa
     6481 gaacgcggcg atgacagtga taaaagaagt ccccgcccgc gattcggcgc tcgagcgaag
     6541 cagcaagctg ccggggcgaa tcgatgcgct cctttggacg ctgctggcgg tgtgcatcgt
     6601 ccggctatgg ctcgcgccgc tcggatcgag tttctgggtg gatgagatgg taacggtctt
     6661 tgtggtgcgc catggtgcgg cccatccgtc actggccgca gctccccagg tagggcgcag
     6721 cgtttactat tttttgccca aggccgcgga ggcgctcctt ggcttttccg agatctctta
     6781 ccgtgttccg tcgatcctgg cgatgggcgc ggctgttttt ctgatcagcc ggctagcggc
     6841 gcgtctcatt cacccgcaag cgcagtggtt cgcggctttc gcctgtttgg cgctgccggg
     6901 cttgaattat gaggcggccg acgcgcgccc ctacgccctg ggcatttgcg ccgcttcggc
     6961 cagctttttc tttctggtcc gctggctcga tttcgcgaga cggcgcgacg cgtttc
//
LOCUS       DASREK010000319        10073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2454206, whole genome shotgun sequence.
ACCESSION   DASREK010000319 DASREK010000000
VERSION     DASREK010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454206"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1212)
                     /locus_tag="VFA54_17470"
     CDS             complement(214..1212)
                     /locus_tag="VFA54_17470"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZS52662.1"
                     /translation="MADRFSRREFLEKSVLAGAAAAAFGAEAENSSQPMPTRILGKTG
                     VPVSILALGCGSRLLSYGNEDAAIAAVNLALDQGIRYLDTAYGYGRGRSETWIGKVAK
                     VRRKDFFLATKIEPRDGDEAQRILDGTLKRLQTDQVDLIHVHALGDEQDLARAEAKNG
                     VLNVLFKLREQKVTRFVGITSHYDPVVLKTAIERHDIDCVQMALNAARQGMTSGAGKM
                     TLNPAMKTSFEQIALPAARKKNLGILAMKIMGQEELLGAGPHKSDPARLLQYTLSLPV
                     AAAVVGMPKPEYIRQNAAWAKAFQPMPAGEMNEFSRRLAGANKAALDHKFRDHIDC"
     gene            1341..2423
                     /locus_tag="VFA54_17475"
     CDS             1341..2423
                     /locus_tag="VFA54_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS52663.1"
                     /translation="MSGSCGVAAVVYNGRFCAEAEVEESFRVGVTADFQTQAKGVIEP
                     AVESVLAGFEWEFLPDTGETARGDVLDRYDAVIALALRFPAESVRWRRRLAVIARWGV
                     GYDRIDVAACTEGGVILAITPEAVRRPVAEGIFTFVFALAKNLRPLDQLCRAGRWRGG
                     APRTSQNIEGKTLGSIGAGNIAGEVFRMARALGFGRLLAYDPLAAAAPEGVEMADLPT
                     VLRESDFVTVNCPLTAQTRHMLGAPELALMKPSAYLINTARGPIVDNDALYEALRSGR
                     IAGAGLDVFETEPVPADNPLLRLENVIVTPHSIAWTQEVFRDNSIQACRNVLEVSKGR
                     APKYVVNREVLDRPECQAKLARWKTA"
     gene            2451..3233
                     /locus_tag="VFA54_17480"
     CDS             2451..3233
                     /locus_tag="VFA54_17480"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZS52664.1"
                     /translation="MKPNLVRQRLHEGRVAVGHMVMEFTTRGIAKILEAADADFALID
                     MEHAANDLPRIADLAAWFKATPVAPLVRVPAADYHFIARCLDAGVLGVMVPNVETAEQ
                     ACAIVNAVKYAPGGHRGLGLGSAHNDYVPPKPAEYLHEANANTSVICQIESTRGLENL
                     EAIAATPGVDILWVGHFDLTQSMGIVAQFDDPRFLDALARVAQVAKRYGKAAGVQPHN
                     AAQAEQWMRLGYNVISWSSDFAVYRNALAEHIATVRRLEAAK"
     gene            3311..4477
                     /locus_tag="VFA54_17485"
     CDS             3311..4477
                     /locus_tag="VFA54_17485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZS52665.1"
                     /translation="MPSIGLASSALRVPYSRIRELAEIAMTMDGVLKLYFGESNLPTP
                     QFIKDAAAEALREGHTFYTSNAGYASLRQAIAEKYGELHGVEIDGLRQIVITASGVQA
                     LNVALRCLLDPGDEAIILTPAWPNSSSIVQMCSAVPRQVPLCLEGGRYHIDFGAVEAA
                     IGPRTRLLLYTSPSNPLGWVAAEGEQQSLLDLCRRRGLWLIADEVYERLYYNGPVAPS
                     ILRKTTPDDAVVVPQSFSKSYCMTGWRLGWLVSRPDLAKKAAELNEFIVSHASAMTQK
                     AGEAALREGEASVAEMVRRLRENRDLALAALKNMPRITVPSPDGAFYLFPRIDGLRDS
                     FEFCRRLLMEKRVGLAPGVAFGAGGEGSVRLCYAADRTVLEPALERLDEFVRSA"
     gene            complement(4516..5841)
                     /locus_tag="VFA54_17490"
     CDS             complement(4516..5841)
                     /locus_tag="VFA54_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZS52666.1"
                     /translation="MSLFDTQPLKPQRPAGRPPLAELMRPETLEDFLGQEQILGPGKP
                     LRVQIERDEISSLILWGPPGAGKTTLARIIARMTRSEFVPFSAVLSGIKEIKAVMAEA
                     EKLRHYGRRTILFVDEIHRFNKAQQDAFLPYVERGDIILIGATTENPSFEINAALLSR
                     CKVYVLRPLTTEEIEELLRRGARKLGVNISDELNRQIAIFSSGDARVAYNTLEAAAAA
                     SQGPEITASAVEDAIQRKILLYDKAGEEHYNLISALHKSVRASDPDAALYWLTRMLEA
                     GEDRLYAARRLMRMAVEDIGLADPHALEQAAAAAQAVHFLGQPEGDLALAQLAVYLAT
                     APKSDAVYAASNRVREDVEKTVAEPVPLQLRNPTTGLMHGIGYGKGYEHAHEFPDAVT
                     AMECLPESLRGRRYYHPTDRGFEASLAERLKEIDEKKRKLREQPPQHKT"
     gene            complement(6059..6856)
                     /locus_tag="VFA54_17495"
     CDS             complement(6059..6856)
                     /locus_tag="VFA54_17495"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZS52667.1"
                     /translation="MIVAKRAAISSLSNPLLKDVRKAIMRGDLTSSGWAVAETFHLLE
                     EARRSGLEIPVVLSTGQVQQELISRENGLPGSRLIVLPDAIFRRLASTENAQGVIALV
                     RLPAWNLEDVFGAAPLVVVLDGVQDPGNAGAILRAGEAFGATGAVFLKGSVSPFNVKT
                     IRASAGSLFRLPFVAGIEVDAVQACLRKHQVSVYAAHPRGNLPLTRASFRAPTAIVIG
                     SEGHGVRDVFLREATLLSIPTMIVESLNAAQAATVVLYEAARQRGVP"
     gene            7025..7651
                     /locus_tag="VFA54_17500"
     CDS             7025..7651
                     /locus_tag="VFA54_17500"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZS52668.1"
                     /translation="MTVTFRVNPAQLNRRAIQQAAAAIRDGKVIAIPTDTVYGLAADP
                     FQPAAVREVFRLKGRPENKPILLLVNSLQQVELLAARLPEAFHSLAARFWPGPLTLIL
                     PAAPSVPDSITAGAGTVAVRLPGSILVRELIGAAHVPLTGTSANRSGRPPSRSAAEVK
                     QQFPSGLPLILDGGPAPSGLPSTLVDLTGEPRIVREGAIPAMDVLSVV"
     gene            7973..8629
                     /locus_tag="VFA54_17505"
     CDS             7973..8629
                     /locus_tag="VFA54_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZS52669.1"
                     /translation="MKMRTLTAARRAESAPWPRPAPVRARWLAFIVLLCAGVFVACFL
                     LVYVQVWQQAGRDEAGPADAIVVFGAAEYRGHPSPVLKARLDHALDLYRRGLAPRIIT
                     TGGHGGEWRFTEGEVGRSYLAQHNVPSEMILVETEGETTMESAAAVAEIMDRMRMSSC
                     IVVSDGYHIYRVKKMIQAHGIRVLGSPRSPGADPPERRAWYYARQALGYMLWRVGIRV
                     "
     gene            complement(8690..9460)
                     /locus_tag="VFA54_17510"
     CDS             complement(8690..9460)
                     /locus_tag="VFA54_17510"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HZS52670.1"
                     /translation="MNCEELRDLYELYAMGAVDPAEKREIDEHLARSCPVCTPAVKQA
                     RGLVSSLALAALPVDPPKLLRERVMDSVVPLPEKPTSVTWLTHAWATLAVLMLAAVIW
                     FVFANQRLEMEIARLNRSVAEAKTVNAELASRNQVLTSALALVILPEARQLVFGRSDR
                     QQPPRGRIWVHPDRGVLLLASDLPPAPAGKAYEMWVVPKGAPPLPAGLFNSDQQGFAA
                     HVWPRPVNVAATSAIAVTLEQATGAAAPTGAPLISASL"
     gene            complement(9457..10026)
                     /locus_tag="VFA54_17515"
     CDS             complement(9457..10026)
                     /locus_tag="VFA54_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZS52671.1"
                     /translation="MILTIVSPDLPDAGLFDGLRRRDPQAMAKLYDAFGKFVYSLIYR
                     IVQDAGVAEDLVQETFLRVWNQAHAIDPSRGSAAAWVLTVARNRAIDWLRSAAGKEGR
                     NLWESYETERPGNFVDVEKAIINSDQARRVREAMAKLNENQRSVIELAYFEGLSQSEM
                     AERMGQPLGTVKTWVRSALKNLREELATP"
BASE COUNT         1811 a         3130 c         3191 g         1941 t
ORIGIN      
        1 aaaagccaca taccttcgtc agcctttgtg cgaatcgctg gaaccgcagc cagagccacg
       61 gcaagtgcta ccgtgctcct gcgcgtcaga cgtcgtctca tactgcgctc ctgggttcta
      121 ttgtaatctg cgagagcttc agggccggcg gagccacagg gcacacgttt tatgggagac
      181 agctccctgt aatgcgccgg cctagggcgc cgatcagcag tctatgtgat cccggaactt
      241 gtgatcgagc gccgctttgt tggcgccggc caatcggcgg gaaaactcgt tcatctcgcc
      301 ggcaggcatc ggctgaaacg ctttggccca ggcggcgttt tggcggatgt attccggctt
      361 gggcatcccg accaccgccg ctgccacggg caggcttagc gtgtattgca gcagcctggc
      421 gggatcgctt ttatggggac ccgcgccgag cagttcttcc tgtcccatga tcttcatcgc
      481 gagaatgccg aggttcttct tgcgggccgc gggcagagcg atttgctcga agctggtctt
      541 catggcggga tttagcgtca tcttgccagc tccgctggtc atgccttgcc tggcggcgtt
      601 gagggccatt tggacgcaat cgatgtcgtg gcgctcgatg gcggtcttca gcaccacggg
      661 atcgtaatga ctcgtgatgc cgacgaaccg cgttaccttc tgctcgcgga gcttgaacag
      721 cacattcagc actccgtttt tggcctcggc tcgcgccaag tcctgctcgt ctcctaaggc
      781 gtgcacatga atcaggtcta cctgatcggt ttgcaggcgc ttcaaggttc cgtccaggat
      841 ccgctgcgct tcatcgccgt ctcgaggctc gatctttgtg gcaaggaaaa aatcctttcg
      901 ccgcactttc gccaccttgc cgatccaggt ctcgctgcgg ccgcggccat acccgtacgc
      961 tgtatcgaga taacgaattc cctgatcgag ggcgagatta acggcggcaa tcgctgcgtc
     1021 ttcgttgccg tagctaagca gccggctgcc acagccgagc gccagaatgg acaccggcac
     1081 tcccgtctta cccaaaatgc gtgtaggcat gggctgcgag ctgttttccg cttcggcgcc
     1141 gaaagcagca gcggctgccc cggccaaaac cgatttttcg aggaactcac gccgtgagaa
     1201 gcgatccgcc atggtgaacc tccggtgatg ttcgttcact tctgatcttg ccgctagtaa
     1261 gcattgtcag cccaagccgc gccaaagtaa agcagccata ggccgaaaag caaagggcgt
     1321 tgtacggctt gaagcgcccg ttgtcgggct catgcggcgt ggcggcagtc gtgtacaatg
     1381 ggcggttctg cgcggaggct gaggtggagg aatccttccg agtgggcgtc actgcggatt
     1441 ttcagacgca ggccaaagga gtgatcgagc cggcggtgga aagcgtcctg gccggtttcg
     1501 agtgggagtt cctgccggac accggggaga cggcgcgcgg cgacgtgctc gaccgctacg
     1561 acgccgtgat cgcgctggct ctccgctttc ccgccgagag cgtcagatgg cgccgccgtc
     1621 tggctgtgat cgcgcgctgg ggcgtcggct acgatcgcat agacgtcgcc gcctgcaccg
     1681 aaggcggggt aatactagct atcacgccgg aagccgtccg ccgccccgtg gcggaaggca
     1741 ttttcacgtt cgtattcgca ctggcgaaaa acctccggcc ccttgaccag ctttgccgcg
     1801 cgggccgctg gcgcggcggg gcgcctcgca ccagccagaa catcgagggc aagaccctgg
     1861 ggtccatcgg cgccggcaat atcgcaggcg aagtgtttcg catggcgcgg gcgctgggct
     1921 tcggcaggct tctcgcctac gatccgctgg cggccgccgc gcccgaaggg gtggaaatgg
     1981 cggaccttcc cacagtcttg cgcgaaagcg atttcgtgac cgtgaattgc ccgctcacgg
     2041 cccagacccg gcatatgctc ggcgccccgg agctggcgtt gatgaagccg tcggcctacc
     2101 tgatcaatac cgcgcggggc ccaatcgtcg ataacgatgc gttatacgaa gcgctgcgca
     2161 gcggccggat cgcgggcgcc gggctcgatg ttttcgagac ggaacccgtg cccgctgaca
     2221 acccgctgct gcgtctggaa aatgtgatcg tcacgccgca ttccatcgcc tggactcagg
     2281 aagttttccg cgacaacagc atccaggcat gccggaacgt gctcgaagtc tcaaagggac
     2341 gtgctccgaa atatgtggtg aatcgcgagg tgctcgaccg gccggagtgc caggcaaagc
     2401 tggcgcggtg gaagacggcg tagaccagat ctcaagcgga aggagggaac ttgaagccca
     2461 atctcgtgcg gcagcggctc cacgagggac gcgtggcggt cggccacatg gtcatggagt
     2521 ttacaacgcg gggcattgcc aaaatcctgg aggcggccga cgcggacttt gcgctgatcg
     2581 atatggaaca cgcggccaac gatctgcctc gtatcgccga tctggcggca tggttcaagg
     2641 ccaccccggt cgctccgctt gtgcgggtgc cggcggcgga ctatcatttc atcgcccgat
     2701 gcctcgatgc gggggttttg ggcgtcatgg ttcccaacgt ggagacagcg gagcaggctt
     2761 gcgccattgt caacgcggtg aaatacgcgc ccggcggcca tcggggcctc ggcctgggct
     2821 cggctcataa cgactacgtt ccgcctaagc cggccgagta tttgcacgag gccaacgcga
     2881 acacctcagt catttgccag atcgagagca cccgcggcct tgaaaatctc gaagccatcg
     2941 ccgcgactcc aggggtagat attctctggg tcgggcactt cgatttgacg cagtcgatgg
     3001 gcattgtggc gcaattcgac gatccccgat tcctggacgc tctggcgcgc gtggcccagg
     3061 tagcgaagcg atacggcaag gcagccggcg tgcagccgca caatgctgcc caggcggaac
     3121 agtggatgag gcttggttac aacgtgattt cctggagctc cgattttgct gtgtatcgca
     3181 atgccttggc cgagcacatc gccacggtgc ggcgtctcga agcggcaaaa tgaccggccg
     3241 gcgctcccgc gcggggcagc gttcccccag cgaaacgccg tttctttttc cggggttatg
     3301 atgaaagaca atgccatcca tcggcctcgc ctcttcggct ttgcgggttc cctattcgcg
     3361 tatccgggaa ctggcggaaa tcgccatgac catggatggc gtcctgaagc tctactttgg
     3421 agaatccaat cttcccaccc cgcaattcat caaagacgcc gcggctgagg ccctgcggga
     3481 gggccatacg ttctatacct ctaatgccgg ctacgcgagc ctccggcagg cgattgccga
     3541 aaaatatggg gagctgcatg gcgtggaaat cgatgggctc cggcagattg taattacagc
     3601 gtctggcgta caggcgctca acgtggcgct ccgctgcctg ctcgatccgg gcgatgaagc
     3661 cattatcctg acgcctgcat ggccgaactc ttcctcgatc gtccagatgt gctcggctgt
     3721 gccgcgccag gttccccttt gccttgaagg cggccgttac cacatcgatt tcggcgcggt
     3781 ggaagcggct atcggccccc gaaccaggct gctcctctat acctcgccct ccaacccgct
     3841 cggctgggtg gcggccgaag gcgagcagca atctctgctc gatctttgcc gccggcgggg
     3901 tctttggctt attgcggatg aagtatacga acgcctgtac tacaacggtc cggtcgcgcc
     3961 gtcgatttta cgcaaaacca cgccggatga cgcagtcgtc gtgcctcagt ccttctctaa
     4021 aagctattgt atgaccggat ggcggctcgg ctggctggtc tcgcgtccgg atctggccaa
     4081 gaaagccgcg gagctgaacg aattcatcgt ctcgcacgcc tcggcgatga cgcaaaaagc
     4141 cggcgaggcc gcgctccgcg aaggagaggc gagcgtcgcg gaaatggtcc gacggctccg
     4201 cgaaaatcgc gatctggccc tggctgcttt gaagaacatg ccgcgcatta cggtccccag
     4261 cccggatggc gcgttttatt tgttcccgcg catcgatgga ctccgggatt ccttcgagtt
     4321 ttgccggcgg ttgctgatgg agaagagagt aggtctcgct ccgggggtgg ccttcggcgc
     4381 gggcggcgag ggatccgtcc gcctatgcta cgccgctgac cggaccgtgc tggagccggc
     4441 ccttgaacgg ctggacgagt tcgtccgttc cgcttgatcg cccccggcgc cggccggcgg
     4501 ttgcatgctc cgccttcacg ttttgtgctg cggcggctgt tcccgcagct tccgcttctt
     4561 ttcgtcgatc tctttcaacc gctcagcaag gcttgcctcg aagccgcggt ccgtcggatg
     4621 gtaataccgc ctgccgcgca gcgattcggg caggcactcc attgctgtca cggcgtcggg
     4681 aaattcgtgg gcgtgctcgt agcccttgcc gtagccgatg ccgtgcatca gtccggttgt
     4741 ggggtttctc agttgcaagg gaaccggctc ggctacggtc ttctccacgt cctcccgaac
     4801 tctgttggat gctgcataaa cagcgtcaga tttgggggcg gttgcaagat acacagcgag
     4861 ctgcgccagg gccagatcgc cttccggctg tcccagaaag tgaacggctt gcgcggcagc
     4921 cgcagcctgc tcaagagcgt gcggatcggc cagaccgata tcctcgaccg ccatccgcat
     4981 cagccggcgc gccgcgtaca agcggtcctc gccggcttcc agcatgcggg tcagccagta
     5041 cagcgcggcg tcgggatcgg aggcgcgaac cgacttatgg agcgccgaga tcaggttgta
     5101 gtgctcttct cccgccttgt cgtacagcag gatcttgcgc tggatggcgt cttcgacggc
     5161 agaggctgtg atctcgggac cttgggacgc ggctgccgcc gcttccaggg tgttgtatgc
     5221 cacccgggcg tctccgctgg aaaagatcgc aatctgacgg ttcagctcgt cggaaatgtt
     5281 gacgcccagc ttgcgggcgc cgcgccgcaa cagttcctcg atctcctctg tggtcagagg
     5341 gcgcagcaca tacaccttgc agcgggaaag cagggcggca ttgatctcga acgacggatt
     5401 ttcggtcgta gctccaatca ggatgatgtc gcctcgctct acgtagggca gaaatgcgtc
     5461 ctgctgggct ttgttgaagc gatgaatttc gtctacgaaa agaatggtcc ggcggccgta
     5521 atgccgcaat ttttcagctt ccgccattac cgctttgatt tccttgatgc cgctgagaac
     5581 cgcgctgaag ggcacaaact cgctgcgagt catgcgggcg atgatgcggg cgagcgtcgt
     5641 tttgcccgcg ccgggtggac cccacaggat aagggaagag atctcgtcgc gctcgatctg
     5701 aacccgtaag ggcttgccag ggccgagaat ttgctcctgg ccgagaaaat cctccagcgt
     5761 ctcgggccgc ataagctcgg ccagcggcgg ccgccccgcc ggacgctgtg gtttgagcgg
     5821 ctgtgtatcg aaaagactca tggtccggct ttcggcgagc gtctgggcgg tcctgggcca
     5881 cgtgttccgg ggcggtaatg aaatcgagcc cgcctgagat aaacgggttc tatcttattt
     5941 cgcgccctag gacgcgggcc gacccttttg gcgcatcgcg gccctctgct ccgatcaccc
     6001 gcgcggtccc tgcctgaacc aacgcgcctg cggccgaaga ggagcgcacc ctggggggct
     6061 atggcacgcc tctttgccgc gccgcttcat agagaaccac cgtggccgcc tgagcggcgt
     6121 tcaaagattc cacaatcatc gtggggatcg acaagagcgt cgcttcgcgc aagaacacgt
     6181 cccgcactcc gtggccttcc gagccgatga cgatcgccgt cggggcccga aagctcgctc
     6241 gcgtcagcgg caggtttccg cggggatgag cggcgtaaac cgagacctga tgcttgcgca
     6301 ggcaggcctg aaccgcgtcc acctcgattc ccgcgacaaa tggcagccgg aacagcgagc
     6361 cggcagaggc gcgaattgtc ttcacgttga acgggcttac cgagcccttc aaaaagacgg
     6421 ctcctgtggc cccgaaggct tcacccgccc gcaggatggc cccggcattg ccagggtcct
     6481 gcactccgtc gagcaccact acgagagggg ccgcgccgaa aacgtcctcc agattccagg
     6541 ccggaagccg caccagggct atcacgccct gggcattctc ggtgctggca agtcttcgga
     6601 aaatcgcgtc aggaagcacg atgagccgcg atccaggcag gccgttctcg cgggatatca
     6661 gttcttgctg cacctgcccg gtcgaaagga ccaccgggat ttccaggccg ctgcgccgtg
     6721 cttcttcgag tagatggaag gtttccgcta ccgcccaacc ggaggaggtg aggtctcccc
     6781 gcatgatggc cttgcgcacg tccttcaata gaggattgga caggctcgaa atggctgcgc
     6841 gctttgccac gatcatttgt ccagccggtt ccggaagagg ccgcaagaat gcgccgaagt
     6901 ctagctgagc tacggcgaag tcccttgccc tgaagtccgg agccgccaca gagcatcccc
     6961 gcaaggcgcc gggcgactta ccggcgacag gacgcctcaa taagaatcgt aatagaattg
     7021 cggcgtgact gtcacattcc gagtcaatcc ggcgcaatta aacaggcgag cgatccaaca
     7081 agccgctgct gctatccgcg acggaaaagt aattgcgatc cccacagata ccgtgtacgg
     7141 attggcggca gatccgttcc aacccgccgc cgtccgagag gtattccgtt tgaaaggcag
     7201 gccggagaac aagccgattc tcttgctggt gaattccctc cagcaagtgg agctgctcgc
     7261 cgccaggctg ccggaagcat ttcactcgct cgccgcacgc ttctggcccg ggccgctgac
     7321 tctcattttg ccggccgccc cgagcgtccc ggacagcatc accgcaggcg ccggtaccgt
     7381 agccgtgcgg ctgcctgggt cgatacttgt gcgggaactg atcggtgctg cgcatgtgcc
     7441 cctgaccgga accagcgcca accggtcggg ccgcccgccc tcgcgttcgg cagccgaggt
     7501 gaagcagcaa tttccctctg gcctgccatt gatcctcgac ggaggtcctg cgccaagcgg
     7561 cctgccctcc acgctggtag acctcaccgg ggagccgcgg atcgtccggg aaggcgccat
     7621 tcccgcaatg gatgtgctat ctgttgttta gcggccacag tgggccgaga cgcgccggca
     7681 gagccgcata aaacctggcc ggaaaacggc aggtcatgtt ttttatgttg cagggacagc
     7741 aggaggcctg gcaggcgttc ccgacgcagg cctcctgcct ggcatggcaa accggtattc
     7801 cacaacgttc gatcaagtgg cggtggtttt gcctgcgtat gatcgcggtg gattcccggc
     7861 gaagcggcgc tgaccgttgg ccctgggatg ctggcgcttg ctgctcgggc tggcaaggct
     7921 ggcggatggg aaactggaga acgctcagag aggattggtc cgagagacat tcatgaagat
     7981 gcggacgctc accgccgcgc gcagggcaga gtctgccccc tggccgcgtc ccgctcccgt
     8041 tcgagcccgc tggctggcct tcattgtgtt gttgtgcgca ggcgtcttcg tggcgtgttt
     8101 tttgctggta tacgtccagg tctggcagca ggccggccgg gatgaagcgg gcccggccga
     8161 tgcaatcgtg gtattcggcg ccgccgaata tcgcggccat ccctcgcctg tgttgaaggc
     8221 ccggctggat catgctttgg atctttaccg gcgcggcctc gccccccgca tcatcaccac
     8281 cggggggcac ggcggcgaat ggcgattcac cgaaggagag gtcgggcgca gctacttggc
     8341 ccagcacaac gtgccttcag agatgattct ggtggaaaca gagggcgaga cgactatgga
     8401 aagcgcggcg gcggtcgccg aaattatgga ccggatgcgc atgagctcgt gcattgtagt
     8461 gagcgacggc tatcacatct accgcgtgaa gaaaatgatc caggcgcacg gcatccgcgt
     8521 gctggggtca ccgcgaagcc cgggcgccga tcccccggag cggcgcgcct ggtattacgc
     8581 gcggcaggcg ctcggataca tgctgtggcg ggtcggcatc cgcgtctgac gttggaatgg
     8641 ccagctcagg ccccaccgaa gaccgggctg cggggcagct cgtcaaggtt cacaggctgg
     8701 cggagatgag aggcgcgccg gtgggagccg cggcgcccgt cgcctgctcc agcgtcaccg
     8761 cgattgccga ggttgccgcg acattgaccg gccgcggcca tacgtgcgcg gcgaaaccct
     8821 gttggtcgga gttgaacaat ccggcgggta aaggcggagc gcctttaggc acgacccaca
     8881 tctcataagc tttgccggcc ggcgcgggag gcaagtcgga ggcgagcaat agaacgccgc
     8941 gatcgggatg gacccatatg cgtccgcgcg ggggctgttg ccggtcagag cggccgaaaa
     9001 caagctggcg cgcctccggg aggataacca gcgccaaagc ggacgtaagg acttggttgc
     9061 gggacgcgag ttccgcattc accgtttttg cctcggccac gctccggttc agccgcgcaa
     9121 tttccatctc cagcctctga ttggcgaaaa caaaccaaat gacagccgcc agcatcaaga
     9181 ccgcgagtgt ggcccaggca tgcgtaagcc aagtcacgct ggtcggcttt tccggcagag
     9241 gcacgactga atccatgacg cgctcgcgga gcagcttggg cggatcgacg ggcagcgccg
     9301 ccagggcgag agaactgacc aggccgcggg cttgcttcac tgccggggta caaaccgggc
     9361 aggagcgcgc caaatgctcg tcgatctcgc gcttttccgc cggatccaca gcgcccatgg
     9421 cgtagagctc gtaaaggtct ctcagttcct cgcagttcat ggcgtcgcca gctcttctcg
     9481 caggttcttc agcgcggaac gaacccacgt cttcacggtg ccgagcggct ggcccatccg
     9541 ttcggccatt tccgattggg ataaaccttc aaaataggcc aattcgataa ccgaccgctg
     9601 attctcgttc aatttggcca tggcttcgcg aaccctccgg gcttgatcgg agttgatgat
     9661 cgccttctct acatcgacga aattccccgg gcgctccgtc tcgtagctct cccagagatt
     9721 tcggccctct ttgccggcgg cggagcgcag ccagtcgatg gcgcgattcc gggccaccgt
     9781 gagcacccag gcggccgcag agccgcggct ggggtcgatc gcgtgagcct gattccacac
     9841 ccgcaagaag gtctcttgga ccaagtcttc ggccacgccg gcgtcctgca cgatccggta
     9901 tatcagggag tagacgaact tgccgaatgc atcgtacagc ttggccatcg cctgggggtc
     9961 gcgccgccgc agcccgtcaa acaatcccgc gtcgggaagg tccggagaga cgatagtcaa
    10021 gatcatagcg gactcaacgc tgcggctccg ttcataacac tatacgtctc tca
//
LOCUS       DASREK010000320         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_647472, whole genome shotgun sequence.
ACCESSION   DASREK010000320 DASREK010000000
VERSION     DASREK010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647472"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFA54_17520"
     CDS             <1..533
                     /locus_tag="VFA54_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HZS52672.1"
                     /translation="ALYGLALLGIEDPTGRDRKRLITFVEIDRCATDAVSVVTGCRMG
                     KRALKFRDFGKVAATFVDLAGDRAVRVAARDSSKRLAAERFPEIADRNQQQMKAYRML
                     TDEELFTYQWVKVKLAPQDLPGWRGEKVVCSECGETVNFGREIVRGGRALCKACAGET
                     YYLVLEKGTPPGGRRR"
     gene            581..784
                     /locus_tag="VFA54_17525"
     CDS             581..784
                     /locus_tag="VFA54_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52673.1"
                     /translation="MNPFPIVKQEWSPGNTREVQAAKIDRQIDVEYDSYRRVYIIDGH
                     EWTIAGNIVKQDGQKYYILECAG"
     gene            772..1467
                     /locus_tag="VFA54_17530"
     CDS             772..1467
                     /locus_tag="VFA54_17530"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZS52674.1"
                     /translation="MRRLIDGAPLFGIAGAYWLGGWVARLVARPSADFLLREWDLRLF
                     GAIPGLWMQQHLPRPALDGLELFYFSYYLFVPLAPLLIFVRHGRTGLWQLWISGGLAY
                     AVCDLISPWFPSTPPRLLWSGFASGGWPQAMNLFVLNRFSIGANVFPSSHVAGTTAFA
                     LCHCRHGSCWFLPWALGIAASTVSGGYHYGVDAAAGIAAALAADWGGAEIFRRLRTSR
                     TKALEQRPAPKSN"
     gene            complement(1459..>2577)
                     /locus_tag="VFA54_17535"
     CDS             complement(1459..>2577)
                     /locus_tag="VFA54_17535"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HZS52675.1"
                     /translation="NKLGISIDLKTPAGRDLCLRLAEKVDVLVENFRPGSLARLGLSY
                     EHVRARNPRLIYCSISGYGQNGPSRDLPAMDLIMQAASGLLSITGSENGRVARCGHSV
                     ADITAGMFAAVGILLALHARERSGQGQFIDVSMFDGMISAMCSNFSYFLGSGDIPSPM
                     GTAFKTIVPYRTFATADRDIALAAGSDKLWTSFCEAIGAPHLADDPRYTTNPQRVQNR
                     HILEPLLAKIFRQAPCAEWLSRLSRAGVPCSPVRTFEEVWADPQSSARQMFPALSHSS
                     AGNVTVTGLPVKLSATPGRIRFAAPRRGQHTRAILRSLLCMDDAALDRLESEGVIAGD
                     GKACKSARPGKLRRSRGEAGKNNHSTLRCPEAPDTQSI"
BASE COUNT          473 a          777 c          834 g          493 t
ORIGIN      
        1 tggcgcttta cggcctggct ctgctgggca ttgaagaccc cacgggccgc gaccgcaagc
       61 ggctgattac gtttgtcgag atcgatcggt gcgccaccga tgccgtctcc gtggtgactg
      121 gctgccggat ggggaagcgg gccctgaaat tccgggattt cggaaaagta gccgccacct
      181 ttgtcgatct cgctggggac cgcgccgtgc gagtggccgc ccgagacagc tctaaacggc
      241 tggccgccga gcgcttcccc gagatcgccg atcggaacca gcagcaaatg aaagcctacc
      301 ggatgctcac cgacgaggag ctctttactt atcagtgggt gaaggtaaag ctggcgccgc
      361 aggatctccc ggggtggagg ggagaaaaag tcgtgtgctc cgagtgcggc gaaaccgtca
      421 atttcggccg ggagattgtg cgcggcggcc gcgctctctg caaggcctgt gcgggcgaga
      481 catattacct ggtgctggaa aaaggaacgc cgcccggcgg gagaaggcgg taaggacatc
      541 cggacccgca gagcgatcag ggttctgtcc taggcgcaat atgaatccat ttccaatcgt
      601 gaaacaagag tggtctcccg ggaatacgcg cgaagtgcaa gccgcgaaga tcgaccggca
      661 gatcgatgtt gagtacgact cctatcgcag ggtctacatc atcgacgggc atgagtggac
      721 catagccggc aacatcgtga aacaagacgg ccagaagtat tacatcctgg aatgcgccgg
      781 ctgatcgatg gggcgccgct gtttggaatt gccggcgctt attggctcgg cggctgggtg
      841 gcgcgattgg tcgcgcggcc cagcgccgac tttctgctgc gggaatggga tctccggctg
      901 tttggcgcga tccccggact gtggatgcag cagcacctac cgcggcccgc cctggacggc
      961 ctcgagcttt tctatttctc ctactacctt tttgttccgc tggcgcccct gctcattttt
     1021 gtgcgccacg gccgcacagg gctgtggcag ttatggatca gcggtgggct ggcctacgca
     1081 gtttgcgatc tgatatctcc gtggtttcct tcgacgccgc cgcgcctgct ttggtccggt
     1141 ttcgcctccg ggggatggcc ccaggcaatg aatctttttg tgttgaaccg gttcagcatc
     1201 ggcgcgaacg tctttcccag cagtcatgtc gccggaacta cggcgtttgc cctttgccac
     1261 tgccggcatg gaagctgctg gtttctgccg tgggcgctcg ggatcgcagc ctcgactgtt
     1321 tccggcggat accactacgg agtggacgca gcggccggca ttgcggccgc gctcgcggcc
     1381 gactggggcg gggcagaaat cttccggcgc ttgcggacca gccgcaccaa ggcgcttgag
     1441 cagcgtcccg cgcccaaatc aaattgattg ggtatccggc gcctcagggc agcgcagtgt
     1501 gctgtgattg ttcttgccgg cctctcctct ggaccgccgc agcttcccag ggcgcgcgct
     1561 cttgcatgct ttcccatcgc ccgctatcac accctcggac tcgagccggt cgagcgcggc
     1621 gtcatccata cagagtaacg atcgcagtat ggcgcgcgtg tgctggccgc gcctgggcgc
     1681 ggcaaaacgg atccggccgg gcgtggcgga caatttcacc ggcagcccgg tgaccgtaac
     1741 atttccggcc gaagagtgac ttaaggcggg aaacatctgc cgggcgctcg attgtgggtc
     1801 ggcccagacc tcttcgaacg tccgcacagg cgaacaggga acgcccgctc ggctgagccg
     1861 ggatagccat tcggcgcagg gggcctggcg gaaaattttt gccaggagcg gctccaggat
     1921 gtggcgattc tggacgcgct ggggattcgt cgtgtagcgc ggatcatcgg cgaggtgcgg
     1981 cgctccaatc gcttcgcaaa aagaagtcca cagcttatcg ctgccggcgg caagggctat
     2041 gtcgcggtcg gccgtggcga aggtccggta aggcacgatg gttttaaaag cggtgcccat
     2101 cggagacgga atatctccgg agccgagaaa ataggagaaa ttggagcaca tcgccgaaat
     2161 catgccatcg aacatggaga cgtcgatgaa ttgcccttgg ccgctgcgtt cccgagcgtg
     2221 cagcgccagg agaataccca ccgccgcgaa catgcccgcc gtaatgtcag ccacggaatg
     2281 gccgcaacgg gcgactcgac cattctccga gccggtaatg ctcagcaggc cgctggccgc
     2341 ctgcataatc aagtccatgg ccggcagatc ccgggagggc ccgttctgtc catagcccga
     2401 gatcgagcag tagatcagcc gcggattacg cgcgcgaacg tgctcgtagg agagccccag
     2461 ccgcgccagg ctccccggcc tgaaattttc taccaatacg tcgaccttct ccgccagacg
     2521 cagacacagg tcccggcctg ccggggtctt cagatcgatc gagattccga gcttgtt
//
LOCUS       DASREK010000321         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1315552, whole genome shotgun sequence.
ACCESSION   DASREK010000321 DASREK010000000
VERSION     DASREK010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315552"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFA54_17540"
     CDS             <1..499
                     /locus_tag="VFA54_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HZS52676.1"
                     /translation="EGSGDVLGHEILLNADRFTPVDAGLIPTGELRSVKGTPFDFTKA
                     TRIGARIDQDDEQLKFGQGYDMNFVLNSGGGSPALAAQVYEPSSGRFMEVLTTEPGVQ
                     FYTGNHLDGKLHGKQGKVYGKHAGLCLETQHFPDSPNKPNFPSTVLKPGAAYRTTTIY
                     KFSVK"
     gene            complement(554..1729)
                     /locus_tag="VFA54_17545"
     CDS             complement(554..1729)
                     /locus_tag="VFA54_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZS52677.1"
                     /translation="MQPNRWSRRRFLRWSAALPFSAAAIPPDEELVPFDDYSPEFRTE
                     AQGSHPRVKAFDLRRLTSWTTPSEEFFAFHQTETVNAEARAWRLHVAGLVERPAAFSL
                     DDLTRRVDRRDVPSTIECSGNSGDPRIMNGLVSNATWTGVSLAALLKECGIRPEAREV
                     VFLGMDSEPDRKWEAGNAEFSSPHGWSIFVQDALAPEALVALAMNGKPLPAEHGFPLR
                     LILPGWYGMAQVKWLTRVEVIDRRYEGRHMARNYQSLRAVKTPDGTLWLDTSISRNNL
                     KSVIARVTRRRAGERFQYKIAGAAWGGPARVESVEVQIDGGLWRPVRIDYRNGDYAWL
                     LWSLEWKDAVPGRHTLVSRAINGRGDVQPTREELRARLASNREDYSQWPRRVALGGA"
     gene            complement(1847..1935)
                     /locus_tag="VFA54_17550"
     tRNA            complement(1847..1935)
                     /locus_tag="VFA54_17550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1899..1901),aa:Leu,seq:caa)
BASE COUNT          387 a          645 c          639 g          371 t
ORIGIN      
        1 cgaaggctcc ggtgacgtgc tcggccacga gatcctgttg aacgcggacc gcttcacgcc
       61 ggtggatgcg ggcttgattc ccaccgggga gctgcgaagc gtcaagggaa ctccatttga
      121 cttcacgaaa gccacgcgca tcggcgcgcg gatcgaccag gatgacgagc agttgaaatt
      181 cgggcagggc tatgacatga atttcgtgct caactccggc ggcggctcgc cggcgctggc
      241 ggcgcaggtg tatgagccct ccagcggccg tttcatggaa gtgctgacca ccgagcccgg
      301 cgtgcagttc tacaccggaa atcatcttga cggcaagctg cacggcaaac aagggaaagt
      361 gtacggaaaa cacgccggtc tctgcttaga gacccagcat tttcccgact cccccaacaa
      421 gccgaacttt ccgtcaacgg ttttgaagcc gggcgctgcg tatcgaacga ctacgatcta
      481 caaattttcc gtgaaatgat ggaagcggcc ggagccggct gccgcggtcc catccgaacg
      541 gagcgccgcg atttcatgcc ccgccaagcg ccacgcgccg cggccattgc gaataatcct
      601 cgcggttgct ggcgaggcgg gcccgcagct cttctcgcgt tggctggacg tcccctcgcc
      661 cgttgatcgc gcgggagaca agggtatgcc tcccggggac ggcatctttc cattccaagg
      721 accacagcaa ccatgcatag tcgccgttgc ggtaatcaat ccggaccggc cgccagagcc
      781 cgccatcgat ctgcacttcg acgctttcca cacgtgcggg gccgccccag gcggcgccag
      841 ctatcttgta ttggaagcgc tcgccggcgc ggcggcgggt aaccctggct atcacggatt
      901 tcaaattatt ccgcgaaatc gaggtgtcca gccagagagt cccatcgggc gtcttcaccg
      961 cccgcaagct ttggtagttc cgggccatgt gccggccttc gtagcgccgg tcgatgactt
     1021 ccacgcgggt gagccacttg acctgcgcca tgccatacca gccgggcaat atcaggcgca
     1081 gcgggaaccc atgctcggcc ggcagaggct tgccgttcat ggccagcgcg accagagcct
     1141 caggggccaa ggcgtcctgt acgaaaatgc tccagccgtg aggcgatgag aactctgcgt
     1201 tgccggcttc ccatttcctg tccggctcgg aatccatgcc cagaaatact acctcgcgcg
     1261 cttcgggcct gatgccgcac tctttgagaa gcgcagccag gctgacgcct gtccaggtgg
     1321 cgttgcttac caggccgttc atgatgcgcg gatcgcccga gttgcccgag cattcgatgg
     1381 ttgacggcac atcccggcgg tccacgcggc gggtcagatc gtcgagcgaa aaagcagccg
     1441 gacgctccac tagcccggcg acatgcagcc gccaggcgcg ggcctcggcg tttacagtct
     1501 cggtctggtg gaacgcgaag aactcttccg atggagtcgt ccaggaagta agccgccgca
     1561 aatcaaacgc tttcacgcgc ggatggctcc cctgggcttc ggtgcggaat tccggggagt
     1621 agtcgtcgaa ggggacaagc tcctcatcgg gagggatagc ggcggcggag aaggggaggg
     1681 cggcgctcca gcgaaggaat cggcgtcggc tccatcgatt cggctgcaaa ctttcctctg
     1741 gggtagattc tatcaggtct aaaacagagg atcttagttg aggggtcgtc ccggcgcccc
     1801 cgccggcaaa cgagcgacac acgccacgat cggaccttcc gactgttggt gctggaggag
     1861 agggtcgaac tctcatgccc agtgaagggc gcgggatttt gagtcccgtg cgtctgccag
     1921 ttccgccact ccagcggcag tctgcttcga gaataccaga ccgggctctc agcgccggaa
     1981 ctgcttgctc agcgtgagct gctgcgactg gtatgacgac ccgatgcccg ctccgtacag
     2041 cc
//
LOCUS       DASREK010000322        14367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1480105, whole genome shotgun sequence.
ACCESSION   DASREK010000322 DASREK010000000
VERSION     DASREK010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 14367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14367
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480105"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..360)
                     /locus_tag="VFA54_17555"
     CDS             complement(136..360)
                     /locus_tag="VFA54_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZS52678.1"
                     /translation="MPVFMRTDVWLPLWKSMTAQAARLYRNEGGQDLVEYALLAGFVA
                     VAAGALLPGISTSISTIFSRMASVLTAASS"
     gene            complement(403..1884)
                     /locus_tag="VFA54_17560"
     CDS             complement(403..1884)
                     /locus_tag="VFA54_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52679.1"
                     /translation="MAEIVFASVLLWLLLGGQASALLADGDTGWHIRTGDYILSRRTV
                     PRTDLFSFSRPGAPWFAWEWGSDVLFSLAHKAAGLKGVVLLAGILIAATSALLVQYML
                     WLRVNIIVAVLAALIAASASTMHWLARPHLFTGLLFVIIVWLLEADRSRNTAWVWTLV
                     PMAALWANLHGGFLIMIAVLGLYATASALEKTISPGKNWKAAGRYAGLAGLCVLATLI
                     NPYGFRLHEHIWQYVRSDFIRDHVQEFQGPSFRGESMFAFELLLVAGLLSAPSLWRRG
                     ERAPALVLLALAHAALGSVRHVLLYVAAAAPLAAREATRMLESARGVWPGTLARLAAD
                     YAPQTDGASKFHLPWRACAPVALAWALLNSGPALWQADFPATKFPLAAINRAEKILVG
                     RKVFTSDQWADYLIYRFYPRERVFVDGRSDFYGAAIGQDYLDAQQARYRWNEVFQRFG
                     LEMALVSPEEPIATVLKIDPGWRVVCDDGQAVVLEKVQQPSPR"
     gene            2092..2718
                     /locus_tag="VFA54_17565"
     CDS             2092..2718
                     /locus_tag="VFA54_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS52680.1"
                     /translation="MITVCACDSQPIALEGLRCILHASEDLKFVEGAQSLLGGMTLLD
                     RHAPNILLVDRAFGAKPVLDWITSAKFRRPETRVILWATVISDVECFRAFQAGARGIL
                     KKTASISAIFDCLRAVANDQIWTESLYSPKDSTGGIPHSRALTPREHEIINLVTRGMK
                     NREIAEALSIATGTVKIHIMHIFEKTGIRDRFELAMYGLKNNTVGCNQ"
     gene            2788..3795
                     /locus_tag="VFA54_17570"
     CDS             2788..3795
                     /locus_tag="VFA54_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HZS52681.1"
                     /translation="MPHDESLGPIYDALVIGAGPTGLACAIEIQIRGMQVALIEKGCL
                     VNSLYHYPPQMTFFTTPELLEIGNIPMTSIREKPTRTEALKYYRRVAQHYKLDVRQYE
                     RVERIEGEDGDFRVFTSQRYGRKACHRSRKIILATGYYDLPNLLNIPGEDLPKVMHYY
                     GEPHPYYDSDVAVIGGKNSAAIAALELYWTGARVTLIHRRDRLSDHIKYWIKPNIENR
                     LKNGEITGYLNSWVEEITEDCIRVRTPDGIKWLKNDFVFALTGYHPDIEFLRATGIEF
                     DPESQRPRTNPETLESDRAGVYLAGVLVAGMHTNEIFIENGRFHGCQIARDIAARLRR
                     T"
     gene            3804..4649
                     /gene="bla"
                     /locus_tag="VFA54_17575"
     CDS             3804..4649
                     /gene="bla"
                     /locus_tag="VFA54_17575"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HZS52682.1"
                     /translation="MKVTMLSLLLAAPLLGQSGRYPPNWTNPFPPHRVIANIYYVGSE
                     DLASYLITTPAGHILINSNLEESVPQIRAGVEKLGFRFSDIKILLGSHAHLDHMAGHA
                     LIKKLTGAQVLVMQGDEGIIRSGGQGDFQYNSHWTACPVDRVLHDGDEVKLGGFTLIA
                     RHTPGHTRGCTTWTTAVNENGRSYNVVIVGSPNVNAGYRLVENAKYPEIAEDYAHSFN
                     IWKSLPCDVFLGAHGGYYGLAAKYRRNQENPGVNAFIDPEGYRAYIAERERAYLQELE
                     RQRER"
     gene            4745..5881
                     /gene="bshA"
                     /locus_tag="VFA54_17580"
     CDS             4745..5881
                     /gene="bshA"
                     /locus_tag="VFA54_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HZS52683.1"
                     /translation="MRVGITCYPTYGGSGIVATELGMELAARGHEVHFISYANPIRLN
                     TDSPRIHYHEVEVSTYPLFQYPPYCLALASRMAEVAETARLDLLHVHYAIPHAISAFL
                     AKEMLAKQARKRRRLPFVTTLHGTDITLVGADRSYLPITRFSIEQSDGVTAISQYLKE
                     HTLSVFGVGEQIRVIQNFVNCDVYRGNPDPAARKAFAPEGEKILVHLSNFRPVKRVLD
                     AVRVAAKVKPDVSIKLLMIGDGPDRGPAEYLARDLGLENDVLFLGKQSRIPELLAVSD
                     VLLLPSEMEAFGLAALEAMACGVPPVATRTGGVPELITDGVDGYLAPVGDTDAMAAYV
                     VSLLTDRDRYARFSQAARRTAETRFSTSLIIPKYEEYYRQVLDR"
     gene            complement(5944..8106)
                     /locus_tag="VFA54_17585"
     CDS             complement(5944..8106)
                     /locus_tag="VFA54_17585"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HZS52684.1"
                     /translation="MRSILYIAILCAGTVFAQNPAVTVAVDAGAGRHPISPYIYGLAY
                     ATTSQLNDLNVPINRQGGNNTSRYNWQLNGDNRGNDWYFESIGDSSSTPGERGDTFIS
                     NCKAAGAQAMLTVPTIGWVAKLGSNRSKLCSFSIAKYGAQTGDDWQWFPDAGNGIYTN
                     GQYVTGNDPNDANVPSDSNFQQGWMQHLTSVWGPAAGGGLRYYIMDNEPSLWFSTHRD
                     VHPAGPTMDEVLSKTIDYAGKVKANDPSALVVGPEEWGWSGYFFSGYDQQYGNLHGWS
                     YLPDRANHGGWDYLPWLLDQLRQNNANTGQRLLDVFSVHYYPQGGEFSNDTSTSMQLL
                     RNKSTRSLWDPNYVDQSWINDKVQLIPRLHNWVNTYYPGTQIAITEYNWGAESHINGA
                     TAQADILGIFGREGLDIAARWTTPDPSTPTYKAIRIYRNYDGNKSAFGDVSISDIVPN
                     PDNLSSFAALRSSDGALTIMAISKVLSGNTPVTFNVANFATQGPAQVWQLTAANVINR
                     LSDISVTGSSFTAALPPQSITLFVLPASGTANQPPAAVLSASPTSGTAPLAVSFDGGS
                     SSDPDGTIASYAWNFGDGGTGSGATISHTYAAPGTYTANLTVTDNAGAASSASTVITV
                     NAPPTLNAPSNLAASVPNKSHTVTLRWTDNSNSENGFYIERAVSARTLSFARVGQTGA
                     NVTTYSETVPSGTYVYRVQAFNSTLVSAYSNQAAARVK"
     gene            complement(8168..9733)
                     /gene="ftcD"
                     /locus_tag="VFA54_17590"
     CDS             complement(8168..9733)
                     /gene="ftcD"
                     /locus_tag="VFA54_17590"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HZS52685.1"
                     /translation="MGCLGPGRRGDAKLRRLIECVPNFSEGRSSAVVDQIAAAICGAP
                     GAIVVDREMDADHNRSVITFVCPVDAALEAAVAAVSKAAELIDLTRHTGAHPRIGAAD
                     VLPFVPLEGVSLQECVELAWQVGEEIWRRCRIPVYYYEAAAKRPERVKLENIRRGQFE
                     GLREEVLRNPARRPDVGGPSLHPTAGATVVGARKFLIAYNINLATADVAVAKAIARKI
                     RESSGGLPCVKAMGVDLKARNRAQVSMNLTDFERTPLHVVFEAVRREAEEQGTEIEGS
                     EIVGLVPKKALEMCAAHFLKIENFDPGLVLENRLAKVMPEAGASPPPADGRQPASGLH
                     AFLDALAFPAATPGGGSAAGAAAAMAAALGAMVAGLAAKKTPDSRELDQLAGQFQSAR
                     TFFEEAVWRDAASYDAVLAAYRIPKERRAGELEAALQTAACVPLEVAEALGPVRESLR
                     RLREVAPVSMHSDIDAAEALALAAWQGARANVAINLASIADASFRTQAERRLEAAAPK
                     EKNVPRLAGTGKS"
     gene            complement(9691..10884)
                     /gene="hutI"
                     /locus_tag="VFA54_17595"
     CDS             complement(9691..10884)
                     /gene="hutI"
                     /locus_tag="VFA54_17595"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZS52686.1"
                     /translation="MAIIQDGALLVVGEKIECVGPSRRLENIKEARGAEVIDATGKVV
                     MPGFVDSHTHFVFPAPRLADFERRIQGASYLELEQAGGGILSTVQALRATSPQTLETK
                     ALRWLRMFAACGTTTVEGKSGYGLTPAAERKILRVMKRVDCRPIEVVRTFLGAHVPPA
                     EFRQNPDDYVEVLTREALPEIRRRHLAEFCDVFCDSGGFDVPQARRILTAAKALGFGL
                     KIHADQLTSLGAVRLAVELGAKSADHLDKLSGRELDLLAASNTIATLLPGSVFHLASG
                     CYPPARALIERGAAVALATDFNPGTSPTLNMQMILALACSQMKMTPAEAVSAATINGA
                     AALGRAAQIGSLEPDKFADLIMLDAGDYREIAYYFGMNLVRLTMRRGQIVWDASAPGG
                     EETQN"
     gene            complement(10986..12644)
                     /gene="hutU"
                     /locus_tag="VFA54_17600"
     CDS             complement(10986..12644)
                     /gene="hutU"
                     /locus_tag="VFA54_17600"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZS52687.1"
                     /translation="MLETQTVRARTVRAPRGPKLSCKGWGQEAALRMLMNNLDPDVAE
                     RPADLIVYGGAGKAARNWECFERIVECLQRLENDETLLVQSGKPVGIFTTHPMAPRVL
                     IANANLVGHWSNWETFHDLDRAGLTMYGQMTAGSWIYIGTQGILQGTYETFAAAARRH
                     FGGTLAGKLIVSGGMGGMGGAQPLAATMNGGAFLGIEVDPERIKRRERSGYCDIMVNS
                     LEEAVRILRSAVHEKKALSVGLVGNCADILPEMVRRGIVPDLLTDQTSAHDPLQGYIP
                     SGYSVAQAAELRARDPKAYTEAALDSIATHVRAMLDLQKMGAVVFDYGNNIRTMAFQR
                     GVANAYDFPGFVPAYIRPLFCEGRGPFRWVALSGEPGDIAATDDLVLDLFPENESLAR
                     WIRLAQKRVKFQGLPARVCWLGYGERARFGLAVNDLVARGRLKAPIVIGRDHLDCGSV
                     ASPFRETEGMRDGSDAVADWPILNALLNTAAGASWVSVHNGGGVGIGYSLHAGQVTVA
                     DGTADGAGRIERVLTCDPGIGILRHADAGYEEALRFAQLAGLKI"
     gene            12854..13732
                     /locus_tag="VFA54_17605"
     CDS             12854..13732
                     /locus_tag="VFA54_17605"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZS52688.1"
                     /translation="MLSFQQMQSFHQMRRRILALALGAAAACAGCTARRYPVSGLVLR
                     VDKKQQAILVSHGKIPGYMDAMAMPFRVRDAGVLDMLRPGMRVEFQLVVGKEGSYAEG
                     VQLQGGGQMAADPNLPAPAPGNSIAVGDAVPDFELTDQAGRKVKFSGFAGKVVAVTFV
                     YTRCPLPDYCPRLSSNFADLQKRFSDRLGRDLFLFTITLDPQYDRPEVLARYARGWKA
                     DADGWRFLTGPMPEIRRVCGLFGVEFWPDQGQITHSLRTGVIDRRGRLAANLSGNDFT
                     PKELCDLVDAVLHGSS"
     gene            13904..14116
                     /locus_tag="VFA54_17610"
     CDS             13904..14116
                     /locus_tag="VFA54_17610"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZS52689.1"
                     /translation="MRSFACLLLVTSGSLHAQQVLRCTPYMPVHVPGALFSVGDGHAA
                     QGDGEVNLTAIESPLTGVFRLRFERT"
     gene            14113..14364
                     /locus_tag="VFA54_17615"
     CDS             14113..14364
                     /locus_tag="VFA54_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52690.1"
                     /translation="MKLRWPRAETPTHIITMGFHETLDEAARRAAKEMIHYLMTARGL
                     SRDDAYMLCSAAVDLQVTQVVDGVKDVHAMLPKSVFGSR"
BASE COUNT         2828 a         4537 c         4247 g         2755 t
ORIGIN      
        1 cagcgcagcg ctccaaagcg ccttttttat cctgtcaata ggcaacttcc tcattgcttc
       61 cttctccttt tccctttcgg gttgtctgct ggtttcccgc ccgccgagag tttctggacc
      121 agcaagccag aaaatttagg atgatgcggc agtcagcacg ctggccattc gggaaaagat
      181 cgtgctgata ctcgtactga tacccggcag aagggcgccc gccgctacgg cgacaaaacc
      241 cgccagcagc gcgtactcca ccagatcctg gccgccctca tttcgataaa gacgggctgc
      301 ttgcgcagtc atcgatttcc aaagcggaag ccacacatcc gttcgcatga ataccggcat
      361 aaatctcctt ttctttgcaa agttccccac aaaggcaacc gtctaccggg gggagggctg
      421 ctggaccttc tccagcacca ccgcctgacc gtcgtcgcac acgacgcgcc agccgggatc
      481 gattttgagc accgtggcga tcggctcctc gggagacacc agagccatct cgagaccgaa
      541 ccgttgaaac acctcgttcc atcggtatcg agcctgctgg gcgtccaagt agtcctgtcc
      601 gatcgcagcg ccataaaagt cgctgcgccc atccacgaac acccgttccc ggggatagaa
      661 gcggtagatc aagtaatccg cccattggtc cgacgtaaac accttccgcc ccacaagtat
      721 cttttcggcc cgattgattg cggccagagg aaattttgtt gcgggaaaat ccgcctgcca
      781 gagggccggc ccggagttca gaagagccca ggccagagca accggagcgc aggcccgcca
      841 cggcaaatga aacttcgagg cgccgtccgt ttgcggcgca taatcggcag ccagacgagc
      901 cagcgtccct ggccatacgc cgcgggcgga ctctagcatc cgcgtagctt cgcgcgcagc
      961 cagcggcgcc gccgcggcca catagagcaa aacatggcgg acagacccga gagccgcatg
     1021 cgcgagcgcc agcagcacca aagccggagc gcgttctccg cggcgccaca gcgacggcgc
     1081 cgacagcaag cccgccacca gcagcagctc gaaggcaaac atgctctctc cgcggaagga
     1141 aggcccctga aactcctgca catgatcccg gatgaaatcg gatcgcacgt attgccagat
     1201 gtgttcatgc agccggaatc cataagggtt gatcagtgtc gccaggacgc acagccccgc
     1261 cagcccggcg tagcggccgg ccgctttcca atttttgccg ggcgaaattg tcttttcgag
     1321 cgccgaggcg gttgcataga ggccgagaac cgcaatcatt atcaagaacc cgccatgcaa
     1381 gtttgcccac agcgcagcca ttgggacaag cgtccatacc caagccgtgt ttcggcttcg
     1441 gtccgcctcc agcaaccaaa caataattac aaacaacaaa cccgtgaaca ggtgcgggcg
     1501 cgccagccag tgcatcgtag aagctgaagc ggctatcagc gcggctagca cggcaacgat
     1561 gatgttcacg cgcagccaga gcatgtactg gaccagcagt gcggaagttg ctgcaatcaa
     1621 aattcccgcc agcaacacga ctcctttcaa accggcggcc ttgtgcgcaa gggagaacaa
     1681 cacgtcgctt ccccattccc aggcaaacca aggggctccg ggtcgtgaaa acgagaacag
     1741 atcggttctg gggacggtgc gtcgcgaaag aatatagtcg ccggtgcgaa tgtgccagcc
     1801 ggtatcgccg tccgccagca gcgcgctggc ctgtcctccc aggagcagcc acagcagcac
     1861 actcgcaaac acaatttccg ccattgaagg cagccaccat ttcgccggcg aggtgcgttg
     1921 ccgagcttct ggctttgcag cggtaggagc ggaaaccggc atcatgcgca ccgaacaggt
     1981 ccgcttatct atcgtcggga gaggaccccc gcatgaggcc gccgggcccg cgaagctcta
     2041 cagtgagacg ctccggcggc cgattgctgt ttgcggaggg aacaatcgtc gatgatcaca
     2101 gtttgcgcct gtgactcgca gcccatcgct ttagaggggc tgcgctgcat actgcacgcc
     2161 agcgaggatt tgaagtttgt ggaaggcgct cagagcctgc tgggcggcat gactctgctg
     2221 gaccgccatg cgcctaatat tctgctggtg gaccgggcgt tcggcgccaa gcccgttctg
     2281 gattggatca cgtcggccaa gttccgccgc ccggagacgc gcgtgattct ctgggccacg
     2341 gtcatctcgg acgtcgagtg cttccgcgcc ttccaggccg gagcgcgcgg cattttgaaa
     2401 aaaaccgcct ccatcagcgc cattttcgat tgcctgcggg cggtcgcaaa cgaccaaatc
     2461 tggaccgaga gcctctattc tccgaaagac tcgaccggcg ggatcccgca ctcgcgcgcc
     2521 ctcacgcccc gggagcatga aatcatcaac ctcgttaccc gcggcatgaa gaaccgggaa
     2581 atcgccgagg ccctttccat cgccaccggc acggtgaaaa tccacatcat gcacattttc
     2641 gaaaagacgg ggatccgcga ccgcttcgag ctcgccatgt acggcctaaa gaacaatact
     2701 gtcggctgca atcaatagca tccgcgagcg gcgttgttcc tggccccggc ctgagcatcc
     2761 caggcatcgg tcggttacaa tggaaaaatg ccgcatgacg agagtcttgg cccgatatac
     2821 gacgctctgg tcataggcgc cgggccgacc ggcctggcct gcgccatcga gatccagatc
     2881 cggggcatgc aggtcgcact gatcgaaaaa ggctgcctgg tgaattctct ttatcactat
     2941 ccgccccaaa tgacgttttt tacaacgcca gaactgctcg agatcggcaa cattccgatg
     3001 accagcatcc gggaaaagcc gactcgcacc gaagcgctca aatattaccg gcgggtggct
     3061 cagcactata agttggatgt ccgccagtac gagcgggtgg agcgcattga gggcgaggac
     3121 ggcgattttc gcgtgttcac ctcccagcgc tacggacgca aggcctgcca ccgcagccgg
     3181 aaaattattc tcgccaccgg ttattacgat ctgccgaact tactgaatat tcccggcgaa
     3241 gatctcccga aggtcatgca ctactacggc gagccgcatc cctattacga ctcggacgtg
     3301 gccgtcatcg gcggcaagaa ttcggcggcc atcgccgccc tcgagcttta ttggacagga
     3361 gcgcgcgtca cgctgattca ccgccgcgat cgattgagcg atcacatcaa gtactggatc
     3421 aagccgaata tcgagaaccg gttgaaaaac ggcgagatca ccggatatct caattcctgg
     3481 gtcgaggaaa tcaccgaaga ctgcattcgc gtgcggacgc cggatggaat caaatggcta
     3541 aaaaatgatt tcgttttcgc gctgaccggc tatcacccgg acatcgagtt cctgcgggcg
     3601 acgggaatcg agtttgatcc cgagagccag aggcctcgca ccaaccccga aacgctcgaa
     3661 agcgaccgcg ccggcgttta cctggcgggc gtgctggtgg ccggcatgca caccaacgag
     3721 atctttatcg agaacggccg cttccacggc tgccagatcg cgcgggatat cgccgcccgc
     3781 ctccggcgaa cctgaaaacg aatatgaaag ttacgatgct gtctttgctg ttggcggcgc
     3841 cgctgctcgg tcaatcggga cggtatcccc ctaactggac gaaccccttt ccgccccacc
     3901 gcgtcatcgc caacatttac tatgtcggct ccgaggatct ggccagttac ttgatcacga
     3961 ctccggccgg gcacatcctg atcaacagca atctcgagga gagcgtaccg cagattcggg
     4021 ccggggttga aaaattgggt tttcggtttt cggatatcaa aattctcctc ggcagccacg
     4081 cccatctgga tcacatggcc ggtcatgccc tgatcaaaaa gctgaccgga gcgcaggtgc
     4141 tggtgatgca aggcgacgaa ggcatcatcc gcagcggcgg ccaaggtgat ttccagtaca
     4201 actcccactg gacagcctgc ccggtcgacc gggtgctgca cgacggcgac gaagtcaagc
     4261 tcggcggctt cacgctgatc gcccgtcaca cgccgggcca cacccgcggc tgcacgacgt
     4321 ggaccacggc cgtaaacgag aacggcaggt cctacaatgt agtgatcgtc ggcagcccca
     4381 atgtgaacgc gggctaccgc ctggtggaaa acgcgaaata tcccgaaatc gccgaggact
     4441 acgcccacag cttcaatatc tggaaatcgc tgccctgcga cgtgtttctc ggcgcgcacg
     4501 gcggctatta cggcctggcg gccaagtatc gccgcaatca ggaaaatccc ggcgtcaatg
     4561 cgtttattga ccccgagggt tatcgtgcct atatcgccga gcgcgaacgg gcctatctgc
     4621 aagaactcga gcggcagcgc gagagatagg ccgggcgccg gcaatgggcc gccgatcatc
     4681 cggagaaacg atcggtcggc cgccgcccat ccgctcccgg ttgtatgggt ctttccctga
     4741 tcgtatgaga gtcggcatca cctgctatcc gacctacggc ggcagcggca tcgtggcgac
     4801 cgagctcggc atggagctgg cggcgcgcgg ccacgaagtg cacttcatca gttatgccaa
     4861 tccaatccgg ctgaacaccg atagtcctcg aattcattac cacgaggtgg aggtcagcac
     4921 atatccgctc ttccagtatc cgccgtattg cctggccttg gcttcgagga tggccgaagt
     4981 ggccgagacc gccaggctcg atttgctgca cgtacactac gcgatccccc acgcgatcag
     5041 cgcgtttctg gccaaggaga tgctcgccaa acaggcgcgc aaacggcgca ggctgccctt
     5101 cgtgaccacg cttcacggca cggacattac gctggtcggg gccgaccgct cgtatcttcc
     5161 gataacccgc ttttccatcg agcagagcga cggagtcacg gccatcagcc aatatctgaa
     5221 agagcacacc ctcagcgtat tcggcgtcgg cgagcagatt cgcgtaattc agaatttcgt
     5281 taactgcgac gtttatcgcg gcaacccgga ccccgccgcc cgaaaggcgt ttgcgcccga
     5341 gggcgaaaaa atcctcgtgc atctttcgaa tttccgcccg gtaaagcgcg ttctggacgc
     5401 tgtacgtgtg gcggccaagg ttaagccgga cgtctccatc aagctgctga tgattgggga
     5461 cggacctgac cgcgggccgg cggaatacct ggcgcgagat ctgggattgg aaaacgatgt
     5521 gctcttcctc ggcaaacaga gccgcatacc ggagctgctc gccgtatccg atgtgctgct
     5581 gctgccgagc gagatggagg cgttcgggtt ggcggcgctg gaagccatgg cctgcggagt
     5641 tccacccgtt gccacacgca cagggggcgt cccggaactc atcacagacg gcgtggatgg
     5701 ttatctcgcg ccagttggcg atacggatgc catggccgct tacgttgttt cgcttctcac
     5761 cgaccgagat cgctacgcca ggttttcgca ggcagcccgc cgcacggccg aaacgcggtt
     5821 ctcgacaagc ctcatcattc ctaaatacga ggagtattac cgccaggtgc tggaccgtta
     5881 atccgcccca tcccttttag gaatctgcta gtagcgccta agccggctcg ggtcattacc
     5941 ggattacttc acgcgggctg cggcctggtt tgaataagca gacaccaggg tggaattgaa
     6001 ggcttgcaca cgataaacgt acgttccgct ggggactgtc tcggaatacg tggtcacgtt
     6061 cgcccctgtt tgtcccacgc gcgcaaagct tagcgtcctc gcgctcaccg cccgctcgat
     6121 gtaaaagccg ttctcgctgt tggaattgtc ggtccatcgg agcgtcacgg tgtggctctt
     6181 gttgggaacg ctggccgcga ggttgctcgg ggcattcagc gtaggaggcg cgtttacagt
     6241 aatgacggtc gaagcagacg aggccgcgcc cgcgttgtcc gttacggtca ggtttgccgt
     6301 gtacgtcccc ggggctgcat aggtatggct gattgttgct ccggagccgg tccctccatc
     6361 gccgaagttc caggcatagg aggcgatagt tccgtcggga tccgaggagc tgccgccgtc
     6421 gaacgacacg gccagaggcg cagttccgga ggttggcgac gcagagagga cggccgcagg
     6481 cggctggttc gctgtgcccg aggcgggcag aacgaacagc gtgatgctct ggggcggcaa
     6541 tgccgccgtg aagctggatc ccgttacact gatatcggaa agccggttga tcacgttggc
     6601 cgccgtcaac tgccagacct gcgccggccc ctgggtggcg aagttcgcca cgttgaaggt
     6661 caccggggta ttgccggaga gcaccttgct gatcgccata atcgtgagcg cgccgtcgga
     6721 cgaacgcaat gcggcgaaag aagaaagatt gtcggggttg gggacgatat cggagatgct
     6781 gacgtcgccg aatgcggatt tgttgccatc atagttgcgg tagatcctaa tggccttgta
     6841 cgtcggggtg gatggatcgg gcgtggtcca gcgagcggcg atgtcaaggc cttcccgccc
     6901 gaaaattccc agaatatcgg cctgagctgt agctccgttg atgtggcttt ccgcgcccca
     6961 attgtattca gtgatggcga tctgcgttcc cgggtaatac gtgttgaccc aattgtgcag
     7021 gcgcggaatc agttgcacct tgtcgttaat ccaactctgg tcaacgtagt tcggatccca
     7081 gagagatctg gtcgatttgt tccgcagcag ttgcatgctg gtcgaggtgt cattgctgaa
     7141 ttccccgccc tgcggataat aatgaacgct aaaaacatcg agcagccgct ggccggtatt
     7201 ggcgttgttc tggcggagct gatccagcaa ccaggggaga taatcccagc cgccgtgatt
     7261 ggcgcggtcc ggcaaatagc tccagccgtg caggttgccg tactgctggt cgtatccgct
     7321 gaaaaaatag ccgctccagc cccactcttc gggacccaca accaacgccg agggatcatt
     7381 cgccttgacc tttccggcgt agtcgatagt cttgctcaaa acctcatcca tggtggggcc
     7441 ggccgggtgc acatcgcggt gcgtcgaaaa ccagagactc ggctcgttgt ccatgatgta
     7501 ataacgcagg cctccgccgg cggccgggcc ccacaccgaa gtcaaatgct gcatccagcc
     7561 ttgctgaaaa tttgagtcgg aaggaacgtt ggcgtcattc ggatcattgc ctgtcacata
     7621 ttgaccattg gtatagatgc cgttgccggc gtccggaaac cattgccagt cgtcgccggt
     7681 ttgtgcgccg tatttggcga tggagaagct gcacagcttg gaacggttcg atcccagctt
     7741 ggccacccag ccaatggtgg gaaccgtcag catggcctgg gcgccggcgg ccttgcagtt
     7801 cgaaatgaac gtgtcgccgc gctcgcccgg tgtgctgctc gaatcgccga tgctttcgaa
     7861 ataccagtcg tttccgcgat tgtctccgtt gagctgccaa ttgtaccggc tcgtgttatt
     7921 cccgccctgg cggttgatgg gaacgttcag atcgttcaat tgcgacgtag tcgcatacgc
     7981 caggccatat atgtacgggc ttatgggatg gcggccggcg ccggcatcaa cagcgaccgt
     8041 aacggcagga ttttgagcga aaacagttcc ggcacagaga atggcgatgt agagtataga
     8101 acgcatcgcc caaggctaac aaacgccggc tctgcgatgc tataggatac aggatctata
     8161 ccaggtacta cgacttccca gtcccggcca ggcggggcac gttcttttct ttgggcgccg
     8221 ccgcttccaa ccgccgctcc gcctgagtac ggaagcttgc gtccgcgatc gaggcgagat
     8281 tgatagccac gttggcgcgc gctccctgcc aagccgcaag cgccagcgct tccgccgcat
     8341 cgatgtccga gtgcatcgaa acaggagcga cttctctcag cctccgcaac gattcgcgaa
     8401 ccggtcccag cgcctccgct acttccagag gaacgcaggc cgccgtctgg agggccgcct
     8461 ccaactcccc ggcgcgccgc tccttgggaa tgcgataggc cgcgagcacg gcgtcatagc
     8521 ttgcggcatc gcgccagaca gcctcttcga aaaaggtccg ggcgctttgg aactgcccgg
     8581 caagctgatc aagttccctc gagtccgggg tcttttttgc cgccaggccg gcaaccatcg
     8641 cacccaaggc agcggccatg gctgccgccg ccccggccgc gctgcctccg cccggcgttg
     8701 ccgccggaaa cgccagagcg tcaagaaagg cgtgcaagcc cgaggcgggc tgccggccat
     8761 cggctggtgg cggggaggct ccggcttccg gcatgacctt ggccaggcga ttctccagca
     8821 ccagtcccgg atcgaaattt tctattttca aaaaatgggc ggcgcacatc tccaaagctt
     8881 ttttcgggac cagtccgaca atctcgcttc cttcaatttc ggtcccctgc tcttccgcct
     8941 cgcgccgcac ggcctcaaaa acgacgtgca gcggagtgcg ctcgaaatcc gtcagattca
     9001 tggaaacctg cgcccggttc cgggccttga gatcgactcc catcgccttt acgcagggca
     9061 ggcctccgga agactccctg attttgcgag cgatcgcctt ggccaccgcg acatcggcag
     9121 tagcgagatt gatgttataa gcgatcagaa acttgcgagc cccgactacg gtcgccccgg
     9181 ctgtgggatg caggctgggg ccgccgacgt cgggccggcg cgcgggattt cgcagtactt
     9241 cctcgcgcag tccttcgaac tggccccgcc ggatgttttc gagcttcacg cgctccggcc
     9301 ttttggccgc agcttcgtaa tagtacacag gaatgcggca gcgccgccag atttcctcac
     9361 ccacttgcca ggccagttcc acgcactcct gtagcgacac tccttccaga ggcacgaaag
     9421 gcagaacgtc ggccgcgccg atgcgcgggt gcgctccggt gtggcgcgtc aggtcaatca
     9481 gctcggcggc tttggaaacc gccgccacgg ccgcttccaa ggcagcatcc acggggcaaa
     9541 cgaacgtgat caccgaacgg ttgtgatcgg catccatttc gcgatccact acgatcgcgc
     9601 ccggcgcacc acagatggca gctgcgatct gatccacgac cgctgagctc cgtccttccg
     9661 agaagttcgg cacgcactcg attaggcgcc tcaattttgc gtctcctcgc cgcccggggc
     9721 cgaggcatcc caaacgattt gaccgcgtcg catcgtcagg cgcaccaagt tcatgccaaa
     9781 atagtaagcg atttcgcgat aatctccggc gtccagcata atcaagtcag caaatttatc
     9841 cggctcgaga gagccgattt gagccgcgcg gcccagcgca gcggcgccgt tgatcgtagc
     9901 cgcgcttacg gcttctgccg gggtcatttt catttgcgag caggccagtg ccaggatcat
     9961 ttgcatgttc agcgttgggc tggtgccggg attgaagtcg gttgccagcg ctaccgccgc
    10021 ccctctctcg atcaatgcgc gggccggcgg atagcagccg gaagcgaggt gaaaaaccga
    10081 acccggcagc agcgtcgcaa tcgtgttcga ggcggcgagc agatccagct cccggcccga
    10141 gagtttatcg aggtggtcgg cgctcttggc tcccagttca acggccagcc ggaccgcgcc
    10201 caagctggtt agctgatcgg catggatctt gaggccgaat cccaaagcct tcgccgcggt
    10261 taagattctc cgcgcctggg gaacatcgaa gcctccggaa tcgcaaaaaa catcgcagaa
    10321 ttccgcgagg tgccgcctgc ggatctccgg gagcgcctcc ctcgtgagca cctcgacgta
    10381 atcatccgga ttctggcgga actccgccgg cggcacgtgc gctcccagaa atgttcgcac
    10441 cacctcgatc ggccggcagt ccaccctttt catgacacgc agaattttcc gctcggccgc
    10501 gggcgtcagc ccgtagcccg acttcccttc cacggtcgta gttccgcagg ccgcgaacat
    10561 ccgcagccat cgcagagctt tggtttcgag cgtctggggc gacgtggcgc gcagcgcctg
    10621 cactgttgag aggatgccgc cgccggcttg ttcgagttcg agataggagg ccccctggat
    10681 gcgccgctcg aagtcggcca ggcggggcgc cggaaaaacg aagtgggtat gactgtcaac
    10741 gaagccgggc atgaccactt tgcccgtcgc atcgatgact tcggcgccgc gcgcttcctt
    10801 gatattctcc aggcgccggc tggggcccac gcactctatc ttttcgccga ctaccagcag
    10861 cgcgccgtcc tggatgatcg ccaactctga aaggtggcgt ccgcgcctcg gaacggagcc
    10921 ccgcaaggtc agcaattgcc gcgccccgcg tatgagcagc cgccttggcc tcctgggatc
    10981 gttggtcaga ttttcaatcc cgcgagctgc gcgaaacgca gcgcttcttc gtaacccgcg
    11041 tcggcatggc gcagtatgcc gattccaggg tcgcacgtca gaacccgctc gattctgccg
    11101 gcgccgtcgg cggttccatc ggccacggta acttgcccgg catgcagaga gtacccgata
    11161 cccaccccgc ctccattatg tacagagacc cagctcgcgc cggccgccgt gttcagcagc
    11221 gcgttgagaa tgggccagtc ggcgacggcg tcgctgccgt cccgcatacc ctccgtctcc
    11281 cgaaacggtg aggccacgga gccgcaatcg agatgatcgc ggccgatcac aatgggagct
    11341 ttcagcctgc cccgcgccac caaatcattc accgccagcc cgaacctggc tcgctccccg
    11401 tagccgagcc agcagacgcg tgcgggaagg ccttggaatt ttacgcgctt ttgcgccaaa
    11461 cggatccacc gggcgaggct ctcattttcg ggaaagagat ccaaaaccag atcatctgtg
    11521 gcagcgatat cgcccggctc gccggagagc gccacccacc ggaacgggcc ccggccttcg
    11581 caaaagagcg ggcgtatata cgcgggaaca aagcccggga aatcgtacgc attggccacg
    11641 ccgcgctgaa acgccatggt ccggatgttg ttgccgtaat cgaacacgac cgcgcccatt
    11701 ttttgcaggt ccagcatcgc ccggacgtgc gtggcgatgg aatcgagcgc ggcctcggtg
    11761 tacgccttgg ggtcccgcgc ccggagctcg gcagcctgcg cgacgctata accgctgggg
    11821 atataaccct gtaggggatc gtgagcgctg gtctgatccg tcagcaggtc gggcacgatg
    11881 ccccgccgca ccatctcagg gaggatatcc gcgcaattgc cgaccagacc caccgacagc
    11941 gccttcttct catggacggc gctgcgcagg atgcgcaccg cttcctcgag gctgtttacc
    12001 atgatgtcgc agtagccgga acgttcacgg cgcttgatgc gctccgggtc cacttcgatg
    12061 cccaggaacg cgccgccatt catcgtcgcg gccagaggct gcgctccgcc catgccgccc
    12121 atcccgcccg acacgataag ctttccggcg agagtaccgc cgaagtgcct gcgcgcggcg
    12181 gcggcgaaag tttcgtacgt cccctgcaaa atgccttggg ttccgatgta aatccaactg
    12241 ccagcggtca tctggccata catcgtcagg ccggcgcggt cgagatcatg gaacgtttcc
    12301 caattggacc agtggccgac caggttcgca ttggcgatca ggacgcgcgg cgccatcgga
    12361 tgcgtagtga aaatacctac aggcttgccc gattgcacga gcagcgtctc gtcattttcc
    12421 agccgctgga ggcattctac tatgcgctcg aagcactccc agttgcgagc cgcctttcca
    12481 gccccgccgt aaacaatcag atcggccggc cgctccgcta cgtccggatc cagattgttc
    12541 atcagcatcc gcagcgcggc ctcctggccc cagcccttgc agctcagctt gggtccccgg
    12601 ggcgcccgga ctgtgcgggc gcgaacagtc tgtgtttcga gcattgggac gctgctcctg
    12661 cttcgattct aacatcgagc tgaaagctca aaacggcgcg gaacatcgcg gcagcaacgc
    12721 tttggccgag agaatctacg cgcttcggaa gccccgctca acgaaaaatt acgaacttct
    12781 ctcgttctcg aggcccttgc ccgcgcgggc tcttttcgac gcgcgggctg tccgatcggg
    12841 ccatactttg gctttgttat cctttcaaca gatgcaaagt tttcaccaga tgcggcgccg
    12901 tattctcgcg ctggccctgg gggcggcggc cgcttgcgcc ggctgcacgg cgcgccgtta
    12961 tcccgtctcc ggtttggtgt tgcgagtaga taagaagcag caagccattt tggtttccca
    13021 cggcaaaatt ccgggctaca tggacgcgat ggccatgccc tttcgagtgc gggacgcggg
    13081 tgtgctcgat atgcttcgac ccgggatgcg ggtcgagttc caactcgtgg tcggaaagga
    13141 agggtcgtac gccgaagggg tgcaactgca aggcggcggg cagatggcag ccgatcctaa
    13201 ccttccagcg ccggcgccgg gaaattccat cgccgtggga gacgcagtgc ccgacttcga
    13261 gttgactgat caagccggcc ggaaggtcaa gttttccggc ttcgctggca aggtcgtcgc
    13321 cgttactttt gtatacacgc gctgccctct gccggattat tgcccgcgcc tttcctccaa
    13381 tttcgccgat ttgcaaaagc gcttttccga ccggctgggt cgtgacttgt ttttgttcac
    13441 gatcactctt gacccgcaat acgatcggcc cgaggtgctg gctcggtacg cccggggctg
    13501 gaaagccgac gcggacggat ggcggtttct aaccggccca atgcccgaaa ttcggcgggt
    13561 gtgcgggctt tttggcgtcg aattttggcc cgatcagggc cagattaccc actcgctacg
    13621 caccggcgtc atcgaccgcc ggggccggct cgccgccaat ctttcgggaa atgatttcac
    13681 gccgaaagaa ctgtgcgatc tcgtggatgc cgtgctgcac gggagcagtt gagccccggc
    13741 gcgctctgcc gaaccaggat ttgcttccgg ttgtacgcgg tcagcaccgc atccttgtta
    13801 agaacggctc ctaggccgga agaaaatccg gcggacgcgc tgaagtaagg gaatcccccg
    13861 aacaccgcgg cgcgccgtgc tacaatgcgt gctccaattc aacatgcgat ctttcgcctg
    13921 ccttctacta gttacaagtg gatcgttgca tgcccagcag gtgctgcgct gcacgcccta
    13981 catgccggtg cacgtgcctg gcgccctttt ctccgttggc gacggacacg ctgcgcaggg
    14041 cgatggcgag gtcaatctga ccgccatcga gtctccgctc accggcgtgt tccggttacg
    14101 gttcgaaagg acatgaaact gcgctggccg cgcgccgaga cgcccacgca catcatcacg
    14161 atgggatttc acgagacgct cgatgaagcg gcgcggcgcg ccgccaagga gatgatccac
    14221 tatcttatga ccgcgcgcgg cctctcccgc gacgatgcct acatgctttg tagcgccgcg
    14281 gtggatctac aggtcacgca ggtcgtcgac ggagtgaaag acgtgcatgc gatgcttccg
    14341 aagtccgtct tcgggtcccg ttgaggg
//
LOCUS       DASREK010000323        12931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_979351, whole genome shotgun sequence.
ACCESSION   DASREK010000323 DASREK010000000
VERSION     DASREK010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 12931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12931
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979351"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VFA54_17620"
     CDS             <1..970
                     /locus_tag="VFA54_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52691.1"
                     /translation="YAPPGGQISRTNPLFVRSHDFLGLQASLHTWRSAELFGSGWPSN
                     GGGRLLGSLTGLPYALAEAEQNFLVPTQTQALIWGDLVPQIMLSAKVPRWWQVTPAEQ
                     HWAALHLRLGQELLARAAVEPQFREALFEKLAFQVAPARRYRMARLIETGQVREAIDL
                     TTPSEMYFLAREYLDSDLSKSGARGPVEQEIRTLAETDPAHANPVRISTDFGTPHPTL
                     ANSYRPELLNLPTFPTLMGYSSRIMAESWESNNLYWAALADELHLSPAQLNVLIPQWT
                     EKVVERIFATHLEDWPAVLRSLRAVGDDYRQRTRPQLLGQIKSSVN"
     gene            981..2012
                     /locus_tag="VFA54_17625"
     CDS             981..2012
                     /locus_tag="VFA54_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZS52692.1"
                     /translation="MRTGKPWVILAAALAAAIGCVLLPGQQPPEPAAAPGQAPVFRVK
                     VDLVVLSVTVTNRQGRYVTDLKPSDFRVLEDGIVQKIAFFGEGNQAPVALDGPKGPAA
                     GASSSEGGSPGGSGIDLASDTFVGTNVFILFDTSNFMYRGFVYAEDAIADFVRGLDRA
                     DSIALYTFSRNLNRAAPLTRDRLLVAKGLRQAVAGDDTALYNALLLTLRDAAAVPGRK
                     VVIVFSNGPDNASMLAPDDVRAVAEDEGIPIYVISTSEVNKDPVSGNVFRRITARTGG
                     KAFFAKTWQKQSEAFQSIREELQHVYTVSYYPQPNPNEGWRSITVELTGDAAKKYRIR
                     TRAGYRPKT"
     gene            2078..3403
                     /locus_tag="VFA54_17630"
     CDS             2078..3403
                     /locus_tag="VFA54_17630"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZS52693.1"
                     /translation="MTLAQFFMSNVIIVGAQWGDEGKGKIVDLLSEKFDIVARYQGGH
                     NAGHTVEAGGRKFILQLIPSGILRPGKTAVIGNGVVIDPEALLSEMKTLESAGIEVSG
                     RLLISHRAHVIFPFHRMMEKASEAKPGRISIGTTSRGIGPTYEDKIARRGIRVADLLD
                     RQSFCVQYEQITAEKKIIARALEIDEPLDFDEIRTRYLAHAEKLRPMVGDAAAFVNRA
                     IDEGRSVLFEGAQGTMLDIDHGTFPFVTSSSATAGGACTGAGVSPTRIDAVIGISKAY
                     VTRVGGGPFPTECHGDSGEKLRERGNEFGAVTGRPRRCGWFDAPLLRYTSMLNHFTSV
                     VITKLDVLDHLTEIPVCTGYRYAGRLLDEMPALARVLDQIEPVYEIVPGWNRSTAGLT
                     EYSQLPDAAKKYLDYLEAQTGVEVGSVSTGPEREQTIIKPGSRLENLLR"
     gene            complement(3400..4410)
                     /locus_tag="VFA54_17635"
     CDS             complement(3400..4410)
                     /locus_tag="VFA54_17635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52694.1"
                     /translation="MTATKRSRFRAWRLFRTAQSYQKKGAPRKAAAVYRLALEADPED
                     WGCWLWRGIALAECGEHDQARQHIERAAELKPSGAAPLLFLARAAYDAGRIEEARAAL
                     AHCLKKQENQLAGAFFALCLFHAGETQRAREILHGGLPFAPWLLGRWLAAIEEKAGAA
                     PRDAAQGNEDADSAPARRGARQLRAGMVHLRAERWEKALAAFRSAAAALPGDARAAYG
                     TGVSLYYLGRFEEAKQHLLPVLNRLPEPFSSNAKAALGRVALELGDFTEARIALRRVV
                     AAGGAAPEDCYALGLAFLRAGRRRLAWRAFERCATPNFILERLAETSDPDKAKLRSGE
                     TD"
     gene            4594..5175
                     /locus_tag="VFA54_17640"
     CDS             4594..5175
                     /locus_tag="VFA54_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZS52695.1"
                     /translation="MAHIDSRLLARAAKIKLLLMDVDGVMTDGRLYNVPGIHGDMVET
                     KGFHSQDGISLQWASWVGLESGVISGRDSPALVTRARQCNMKYVYQGQIEKIPIYEKI
                     LGEAGVCDEEVSYIGDDLTDAPILARVGFAAAPVNARAEVKRMVHLVTEAAGGSGAVR
                     EVVEVILKAQGKWEQILRKYGVSEQAHEVQDCP"
     gene            5255..6952
                     /locus_tag="VFA54_17645"
     CDS             5255..6952
                     /locus_tag="VFA54_17645"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52696.1"
                     /translation="MLDLICLAVIVAAGIAIYWRSLHGPFLFDDADLFEARNAVRLQD
                     WHVILTGPRPLLILSYVLNHRLWGFDPFYFRVVSLFLHIVNTIILWRIVRLICDSPGL
                     DGWLNAGARKLIVVFAPLLFLTTPVQTESVAYISSRSEVLAATFYLLGLLVFLSSWRE
                     KRPWAAAAAIAILYGCALGSKQHTVTLPVAILLADYFFLAGGDWRQLKRNWRTYAVVG
                     ALMLAGGSFVAVEVMNVPSAGFHLKDVTWKSYLLTQFRMYFLYLKLLAAPFGLNADYD
                     IQPSRTIFEHGSWLALAGILSLAGAAVWLRKRFVLASFGVLFFFLNLLPTSSFYPLLD
                     YAAERRLYLPCIGFFLAAMALALEWKKPARAMAWALSGVVLVYSIGAYSRSLVWSDSL
                     ALWLDTAQKSPRKWRVYTWLGFEYTKRNRFAEATAAYQKAAELVSHDSTDYAEVLSSL
                     GSTYNNRKMYAEAIQQYQTALKISPNIPVLWTNLALAEIRLNRMDEGWHHLQRAIQIN
                     PLAWEPHLARGNLFFQMGRYDDAIKDYQRVLDLVPGHPDALQNLQAARTMKRHAAGG"
     gene            7254..7886
                     /locus_tag="VFA54_17650"
     CDS             7254..7886
                     /locus_tag="VFA54_17650"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZS52697.1"
                     /translation="METTPETPNPPRPVTRRQFIQSVIAAGVTASAAAYLYRGTGIVH
                     GKASLPGAVERLITLNVNGQLRRVDVMKQETLAMTLRYKLGLTGTKLGCDRSECGACT
                     VLIDDVPHYSCSVLTHTVRGGKITTIEGLAAADGTLHPVQQAVVDEQAFQCAFCMSGF
                     IMATVGYLKVNPNPTRAELAHGISGNLCRCADYNKILNAMMHASELMRRS"
     gene            7888..10371
                     /locus_tag="VFA54_17655"
     CDS             7888..10371
                     /locus_tag="VFA54_17655"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HZS52698.1"
                     /translation="MAYKLIGHNYQTPDLIAKVTGRSRYAEDYRVDGMLFTKLLLSPM
                     PHARVRHIDAREALKMPGVHAILTADELPKVAPPTRGVAAAEEEGPASKGGRGVAVAP
                     ELGLTNEPVYQGEPILAVAAVDELTAAEAVERIKIDFEPLPFCVDPLESLRPGGPNAR
                     LEGNVYYGNVVKTLKWTEEDWKEVEAGRLPMREAPDTWKVGDVDAAMKEAAVIIDETV
                     VCQSASHQCLEPRTSMAYWQNGKLYLHASAQSLAHVSQSVGNWSGVDRTKLVLLGEYV
                     GAGFGGKNPETQMHTAIPALLAKKTGRPVMMRISREEDHYIGRARAGLIMRVRMGFRK
                     DGRMIALDMFVVQDNGPYGRAGDYGTCAVVASANYTPLTMRFRGTAVLTNTPPHAPQR
                     GPGGVQSTVMFEPLICQAARKLGIDEVEIRKINAPATGAEYGPPDRKGSRTQFTSAFA
                     REALDKGAEKFNWAERKKRNGQMRGTKVTGVGVSLANFTAGTIGFDGMIAITPEGKLN
                     IYSGVGNLGTLSVYDTARVAAEVLDMPWEKCEVIWGDTSKHLPWSSRQGGSQTIHATS
                     RANYAAAMDAKQKLQEIAARDLGGRPEDYTIGNQRVFHKGNPGRGMSYAQAAKRAIEL
                     GGKYDGHEVAKDLNVMTKESAKALAGVGLMGAAKDNYGRKGGTQAFVASFAEVEVDVE
                     TGVYKILDYLCVADCGIVVNPRGLEGQLHGGAIQGIGYTRSQKWVYDQEYGVALAKRF
                     HYNRPPTILDIPEKMEWDAVNLPDPQTPVGAKGIAEAAVGAGAAAVRCALAAAIGDDY
                     IRRTPVGIDMILASLEAKRRVDRGLTANV"
     gene            10385..11389
                     /locus_tag="VFA54_17660"
     CDS             10385..11389
                     /locus_tag="VFA54_17660"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HZS52699.1"
                     /translation="MALIHDVMPAFELFQPVSIDDTLALLDRYKKEAWVMAGGLDTFD
                     WLKDRIKRPEVVVDISGVQELKGIKATGDGGVEIGASTTLTEIVRHPLIKEKYPILAQ
                     AAELVASPQIRNQGTIGGNVSQDTRCWYYRSGWSCYRAGGNICYADTPTAINREHAIF
                     DANRCVAVNPSDTAPALIALDAKMAIRSVKRERVVDAEHFFIGPAIDITRMTVLEPGD
                     LLTSIRIPATWAGAQFYFEKVRERQVWDFPIVNVAAVFVVSGGRIDRSRIAVNAVAAH
                     PKRLTSVEAAIAGKPRNEETAKMAGELAVQGAQPLAHNAYKIPLMRNLVKRAIRGEVA
                     "
     gene            complement(11847..12500)
                     /locus_tag="VFA54_17665"
     CDS             complement(11847..12500)
                     /locus_tag="VFA54_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS52700.1"
                     /translation="MTDLLTQSIKLLLVDDHAMFCDGLARILEKEPDMKVVGKCASAA
                     QALSLLPKSGATMVLLDFDLGAERAVDFVAEARQGGFKGQILVVTAGVSDQEAVQLIQ
                     AGVAGILHKHNSAEVLCSVVRQVAAGEVCLEKSYLKPLFRTVDRSAAPTRPTLTDRDK
                     TVLRCIFQGLANKEIGARLQISEGAVKASLRQLFDKLAVRTRAQLVKVALEQYRDQL"
     gene            complement(12512..>12931)
                     /locus_tag="VFA54_17670"
     CDS             complement(12512..>12931)
                     /locus_tag="VFA54_17670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS52701.1"
                     /translation="LQEVLDNLRIIIEPAWTEIDAVVRWRVPQSMPTVLADPHGLLQA
                     FLNLVQNSFRAVKDCDVRELCISVSVQHPKAIVRFVDSGPGIARPERLFQPLQDGAEG
                     VGLGLYVSRAILRSYGGQLRYEPTEKGSCFAVELQTV"
BASE COUNT         2516 a         3905 c         4033 g         2477 t
ORIGIN      
        1 ctatgctccc ccgggaggcc agatcagccg taccaatccg ctgtttgtgc gcagccacga
       61 ctttctcgga ttgcaagcgt cgctgcatac ctggcgctcc gccgaactat tcggcagcgg
      121 ttggccgtcc aacggaggcg gccggctgct cggatcgctc accggcttgc cctacgcgct
      181 tgccgaggca gagcaaaact tcctcgtgcc gacgcaaacg caggctctca tttggggcga
      241 cctggtgccc cagatcatgc tcagcgccaa ggtgccgcgc tggtggcagg tgacgccagc
      301 cgagcagcac tgggccgcgc tgcacctgcg gctggggcag gaactgctag cccgggccgc
      361 cgtcgagccg cagttccggg aggccctctt cgagaaactc gcttttcagg tggcgccggc
      421 gcgccgctat cgcatggcaa ggttgatcga gaccgggcag gtgcgggaag ccatcgacct
      481 cactaccccc tcggaaatgt acttcctggc aagagagtat ctggatagcg atttgtccaa
      541 gagcggcgcc cggggcccag tcgagcagga aatacggacc ctggccgaaa cggatccggc
      601 gcatgcgaac ccggtgcgca tttccaccga tttcgggacg ccgcatccca cgttggctaa
      661 ctcctaccgt ccggaactgc tgaatttgcc caccttccca actctgatgg gctactccag
      721 ccggattatg gcggaaagct gggagtcgaa caatttatac tgggcggctc tggccgatga
      781 actgcacctt tctcctgccc agttgaacgt gctcattcct cagtggacgg aaaaagtcgt
      841 cgagcgtatt tttgcgactc atttggaaga ctggcccgct gtcttgcggt cgttgcgggc
      901 ggtaggcgat gattatcggc agcggacccg gccgcaactg ctcggtcaaa tcaaatcgtc
      961 ggtgaattag ggaggccatt gtgagaacgg ggaaaccctg ggtgattcta gcagcggccc
     1021 tcgcggccgc gattgggtgc gtcttgttgc cgggccagca accgccggag cccgcggccg
     1081 ctcccggcca ggcgccggtc tttcgagtga aggtggacct ggtagtgctc agcgtcactg
     1141 tgaccaaccg gcaaggccgc tacgtcaccg acctcaagcc ctccgatttt cgggttcttg
     1201 aggacggcat cgtacaaaag atcgctttct ttggcgaggg caaccaagcg ccggtggcgc
     1261 tggacggccc caagggaccg gcggctggag cttcctcgtc cgagggcggc agtcccggcg
     1321 gttcgggcat tgacctcgcc tccgacacct tcgtcggcac gaacgttttt attctgttcg
     1381 acacaagcaa tttcatgtat cgaggtttcg tgtacgccga ggacgccatc gctgattttg
     1441 tgcgcggcct cgatcgcgcc gattccatcg ccctgtacac cttcagccgg aatttgaatc
     1501 gcgcggctcc gcttacgcgc gaccgcctgc tagtcgccaa aggcttgcgg caggcggtgg
     1561 ccggagatga taccgcgctc tacaacgcgc tgctgctgac gctgcgcgat gcggccgccg
     1621 tgcccggccg gaaggtggtg atcgtgtttt ccaacggccc tgacaacgcg agcatgctgg
     1681 ctccggacga tgtgcgcgcc gtcgcggaag atgaaggcat tcctatctac gtcatttcga
     1741 ccagcgaagt gaataaggac ccggtctcgg ggaatgtctt ccgccgcatc actgcgcgga
     1801 ccggcggcaa ggctttcttc gccaaaacgt ggcagaagca aagcgaagcg ttccagtcca
     1861 tccgcgagga gttgcagcac gtgtacacgg tgagctacta cccgcagccc aacccgaacg
     1921 aaggctggcg cagcattacg gtggagctca cgggcgacgc ggcgaagaaa taccgcatcc
     1981 gcacccgcgc gggataccgt cccaagacat agaatccggc aagccccggc cacaccgcgc
     2041 cggcctggtt gcggttgctg tgcgctatac ggtacttttg accttggcgc aatttttcat
     2101 gagcaacgta atcattgtcg gggcgcaatg gggagacgag ggcaagggta aaattgtaga
     2161 ccttctctcg gaaaaattcg acatcgtcgc ccgttatcaa ggcggtcaca acgccggcca
     2221 cacggtggaa gccggagggc ggaaattcat cttgcagttg attccttccg gcattctcag
     2281 gccgggcaaa acggcggtta tcggcaacgg cgtcgtaatt gatccggaag cgctgctttc
     2341 cgagatgaaa acgctcgagt cggccggcat tgaggtatcc ggccggctcc tcatcagcca
     2401 ccgggcccac gtgatctttc cgttccaccg gatgatggag aaggcatcgg aagctaagcc
     2461 cggccgcatc agcatcggaa ccacctcccg cggcatcggc ccaacctatg aggacaagat
     2521 tgctcgccgg ggcattcgcg tcgccgatct gctcgaccgc cagagctttt gcgtgcagta
     2581 cgagcagatc acggcggaaa agaagatcat cgcgcgggcg ctcgaaatcg atgaaccgct
     2641 cgatttcgac gagatccgga cccgctatct cgcccatgcg gagaaactgc gccccatggt
     2701 gggcgatgcg gcggcttttg tcaaccgcgc gatcgatgaa ggccgtagcg tcctgtttga
     2761 gggtgcgcag gggacaatgc tcgatatcga ccacgggacg tttccgttcg tcacttcgtc
     2821 gagcgcaacg gccggcggcg cctgcaccgg cgcgggcgtc tcaccaaccc gcatcgatgc
     2881 cgtcatcggc atatcgaaag cctacgtcac tcgtgtcggg ggcggaccgt ttccaaccga
     2941 gtgccacggc gattcgggag agaagctgcg ggagcgaggc aacgagtttg gcgccgttac
     3001 cggtcggccc cggcgctgcg gctggtttga cgccccgctg ctccgctaca catccatgct
     3061 gaaccatttt acttcggtgg tgatcaccaa gctggatgtg cttgatcatt tgaccgagat
     3121 tcccgtttgc accggttacc gctatgccgg acgattgctg gatgaaatgc ccgccttggc
     3181 gcgggtcctc gaccagattg agccggtcta cgagatcgtg cccggatgga accggtcgac
     3241 agccgggctt acggaatact cgcagttgcc tgacgccgcc aaaaaatatt tggactacct
     3301 ggaagctcag acaggggtgg aggtcggctc ggtatcaacc gggccggagc gcgagcaaac
     3361 cattatcaag cctggatccc gtctcgaaaa cctgctgcgc tagtctgttt cgccgctccg
     3421 cagcttggcc ttatccggat ccgaggtttc ggcaaggcgt tcgaggatga aattcggcgt
     3481 ggcgcagcgt tcgaaagccc gccaggccag ccggcgccgc ccggcccgga ggaacgcgag
     3541 gccgagggcg tagcagtctt cgggagccgc ccctccggcg gccaccacac ggcgcagtgc
     3601 tatccgggct tcggtgaaat cgcccagctc gagcgcgacc ctgcctagtg ctgctttggc
     3661 attgcttgaa aacggttccg gcagccggtt cagcaccggc agcaagtgtt gtttcgcttc
     3721 ttcgaaccgc ccgagatagt agaggctgac gcctgttccg taggccgctc tggcgtcgcc
     3781 gggcagagca gcagccgcgg agcgaaacgc ggccagcgcc ttctcccagc gttcggcacg
     3841 caggtggacc atcccggctc gaagctggcg tgcgccccgg cgggccgggg cgctatcggc
     3901 atcctcgttt ccctgagccg catcacgagg cgccgcgcca gccttttcct cgatggccgc
     3961 aagccacctg cccagcagcc aaggagcaaa cggcagcccg ccatgcagaa tctcgcgcgc
     4021 cctctgagtt tcccccgcgt ggaacaggca caatgcaaag aatgcgccgg ccagttgatt
     4081 ttcttgcttc ttgagacaat gggccagcgc ggcccgggct tcttcgatac gtcccgcatc
     4141 ataggccgca cgcgcgagaa agagcagcgg cgccgcgccg gaaggcttca gctccgccgc
     4201 ccgctcgatg tgctgccggg cctggtcgtg ttcgccgcac tcggccagcg cgatgcctcg
     4261 ccagagccag cagccccagt cttccggatc cgcttcgaga gccagccggt acaccgccgc
     4321 ggccttgcgc ggggcgccct tcttctgata actttgcgcg gttcgaaaca gccgccacgc
     4381 ccgaaaacgg gacctttttg ttgccgtcat gggggaatcc ggatgtagag cgccggagat
     4441 ccctgaggaa ttcatactct gccagtggcg cggcgcctca ccgcgtattt caatataata
     4501 acctcccggt tttgagaaac gcagcggcgg ctgcgaagag gcgttcccgg ccttgggaaa
     4561 acgatccatc gaaattgaga tactagcggt tttatggcgc acatagattc ccggctgctc
     4621 gcccgcgcag ccaaaatcaa gctgctgctg atggatgtgg atggcgtgat gaccgacggc
     4681 cgcctctaca acgttcccgg catacacggg gatatggtcg aaaccaaagg cttccattcc
     4741 caggacggca tcagcctgca atgggcaagc tgggtgggct tggagagcgg agtcatcagc
     4801 gggcgggact cccccgcgct ggtaacccgg gctcgccagt gcaatatgaa gtacgtctac
     4861 cagggtcaga tagagaaaat tcccatttat gaaaagattt taggcgaggc gggcgtctgc
     4921 gacgaggaag tcagctacat cggcgatgac ctcaccgatg cgccgattct tgcccgggtg
     4981 ggcttcgccg ccgctccggt caacgcccgt gccgaagtga agcgcatggt ccatctggtg
     5041 acggaagcgg cgggcggcag cggcgcggtc cgcgaggtgg tggaagtcat cctgaaagcc
     5101 caagggaagt gggaacaaat cctgcgaaaa tacggggtga gcgaacaggc ccatgaagtc
     5161 caggactgcc cgtagactag ttcccgccag cagcgagcgg aaagcggctg cactgaaaga
     5221 tcgggcccgc gccggggagc cggactgcgg gcgtttgctg gatctgatct gcctggcggt
     5281 tatcgtggcg gccggtattg ccatctactg gcggagcctc cacggccctt ttctctttga
     5341 cgatgcagat ctgtttgagg ctcgcaacgc ggtgcggctc caggactggc atgtcatcct
     5401 gaccggcccg cgcccgctgc tgatcctttc ctacgtcctg aatcaccggc tgtgggggtt
     5461 tgatcccttc tatttccgcg tggtgagcct gtttttgcat attgttaata ccatcatcct
     5521 ctggcggatc gtgcggctga tctgcgattc cccgggcctg gacggctggc tgaatgcggg
     5581 cgcccggaaa ctgatcgtag tctttgcgcc gctgctgttt ctcaccactc ctgttcagac
     5641 tgaatcggtg gcttacatca gcagccgctc cgaggtattg gcagcgacat tctatctgct
     5701 cggattgctg gtgtttttgt cgagctggcg ggagaaacgg ccgtgggccg cggcggcggc
     5761 gatcgctatt ctttacggct gcgcgctcgg cagcaaacag cacactgtta cgctgcccgt
     5821 ggcgattctg ctggcggatt atttctttct tgcgggcggc gactggcgcc aactgaagcg
     5881 gaactggcgg acctatgctg ttgtgggggc gcttatgctc gccggcggct cttttgtggc
     5941 cgtggaggtc atgaatgtgc cctcggctgg attccatctg aaagacgtca cctggaagag
     6001 ctaccttttg acccagttcc gcatgtattt cttatacctg aagctgctgg cggcgccgtt
     6061 cgggctgaac gcggattacg acatccagcc ttcacggacc atttttgagc acggctcctg
     6121 gctggcgcta gccggcattt tgtctcttgc tggcgccgcg gtttggcttc gcaagcgctt
     6181 cgttctggca tcttttggcg ttttgttttt cttcttgaac ctgctgccga cttcgagctt
     6241 ctatccgctg ctagactatg ccgccgagcg ccggctgtat ctgccctgta taggcttttt
     6301 cctggctgcg atggcgctgg cgctcgagtg gaaaaagccg gcccgggcga tggcctgggc
     6361 gctctccggc gtggttttgg tttacagcat tggagcatac tcgcgaagcc tggtttggag
     6421 cgacagtctc gctctctggc tcgatacggc gcaaaagtct ccccgcaaat ggcgggtcta
     6481 cacctggctc ggattcgagt acaccaagcg caaccgcttc gcagaggcca cggctgctta
     6541 ccagaaggcg gccgaactgg tttcgcacga cagcacggac tacgccgaag tgctgagcag
     6601 cctgggatca acctacaaca accgcaagat gtacgccgag gcgatccagc agtaccagac
     6661 ggcgctgaaa atctcgccca atattccggt tctctggacc aacctcgcgc ttgccgagat
     6721 ccgtctcaac cgcatggatg agggctggca ccacttgcag cgggccatcc agatcaaccc
     6781 cctggcctgg gagccgcatt tggcccgcgg caatttgttt ttccaaatgg gccgctatga
     6841 tgacgcaatc aaagattatc agcgcgttct cgaccttgtc cccggccatc ccgacgccct
     6901 ccaaaatctc caagccgcac gcaccatgaa gcggcacgcc gccgggggtt agctgcgcgg
     6961 ccgcgcggac attgctcgca ccgctggagc gctgccttgt aattctccgg gatctggccg
     7021 gggcaggccg ccgcccggag cggatcgaat gaaccgcctt ccggcgcgaa cacatcatcg
     7081 gattgaagcc tgccccgaac ttcaaccggc ggtccgttcg ctccgccgcg gcgctgctgc
     7141 aaatccttgg aagattatga aacggcagcc attgactttc atctgctatg gagatacgct
     7201 tccaagtgat cgcgttcatt tgatcctgcc ctatggctta gaggaaatct agtatggaga
     7261 ctacgccaga aacccccaat cctccgcgtc cggttacgag gcggcaattc attcaaagcg
     7321 tgattgccgc cggtgttacg gcttcagcgg cagcttacct ctatcgcggc acgggcatcg
     7381 ttcacggaaa agcatctttg ccgggggcgg tcgaacggct gattacgttg aatgtcaacg
     7441 gccagctccg gcgcgtggac gtgatgaagc aagagaccct ggctatgacg ctgcgctaca
     7501 agctggggct gacgggaacg aaactgggtt gcgaccggtc tgagtgcggc gcctgcacgg
     7561 tgctcatcga tgacgtgccg cactactcat gttcggtgct cacgcacacc gtgcgcggcg
     7621 gaaagatcac cacgatcgag ggtctggccg cggccgatgg tacgctgcat ccggtgcagc
     7681 aggcggtcgt ggacgagcag gccttccagt gcgctttctg catgtctgga ttcatcatgg
     7741 cgaccgtcgg gtatttgaag gtcaacccga atccgacgcg agccgagctg gcgcacggaa
     7801 tttccggcaa cctgtgccgg tgcgcggact acaacaaaat tctgaacgct atgatgcatg
     7861 cgagcgaact gatgcggagg tcttgagatg gcctacaagc tgatcggaca caactaccag
     7921 acgccggatc tcatcgccaa ggtgacggga cggtcaagat atgccgagga ttaccgcgtt
     7981 gacggcatgt tgtttactaa gctgctattg agcccgatgc cgcacgcgcg ggtgcgccac
     8041 atcgatgccc gcgaggcgct caaaatgccg ggcgtgcacg ccattctcac ggccgacgag
     8101 ctgccgaagg tcgccccgcc tacgcgcgga gtggccgcgg cggaagagga aggaccggcc
     8161 tcgaaaggcg ggcgcggcgt ggccgtcgcc cctgagcttg gcctgaccaa tgagccggtg
     8221 taccaaggcg agccgattct ggcggtggcg gctgtggacg aactgaccgc ggcagaggcg
     8281 gtcgagcgca tcaagatcga tttcgaaccc ctgccgttct gcgtcgatcc gctggagagc
     8341 cttcgccccg gcggccccaa cgcgcggctg gagggaaatg tttactacgg caacgtggtg
     8401 aagaccctca agtggaccga agaggattgg aaggaagtcg aggcaggcag gctgccgatg
     8461 agagaggcgc cggacacctg gaaagtcggc gacgtggacg ccgcgatgaa ggaagccgcc
     8521 gtcatcatcg atgagacggt tgtctgccag tcggcttcgc atcaatgcct ggagccgcgc
     8581 acctcgatgg cttactggca gaacggaaag ctctacctgc acgcctcggc gcagtcgctc
     8641 gcccacgtca gccagtcggt cggaaactgg tcgggcgtgg accggacaaa gctcgtgttg
     8701 ctcggagagt atgtcggcgc cggctttggc ggcaagaatc cggaaactca gatgcacacc
     8761 gcgattccgg cgctgctggc gaagaagacc ggccggccgg tgatgatgcg gatttcgcgc
     8821 gaggaggacc attacatcgg ccgcgcgcgc gcgggcctga tcatgcgcgt gcgcatgggc
     8881 ttccgcaagg acggccgcat gatcgcgctc gatatgttcg tcgtgcagga caacggtcct
     8941 tacgggcggg cgggcgatta cggaacctgc gcggtggtgg cttcggcgaa ttacacgccc
     9001 ttgactatgc gcttccgcgg cacggcggtc ctgaccaata ccccgcccca cgccccgcag
     9061 cgcggtcccg ggggcgtaca gtccacggtc atgttcgagc cgctgatctg ccaggcggcc
     9121 cggaagttgg gcatcgacga agtggagatt cgaaagatca acgcccccgc taccggggcg
     9181 gaatacggac ctccggatag aaagggatcg cgaacccagt tcaccagcgc tttcgcgcgc
     9241 gaagctttgg acaagggagc cgagaagttt aactgggccg agcgcaagaa gcgaaacgga
     9301 caaatgcgcg gcaccaaggt caccggcgtc ggcgtatctt tagccaattt cacagccggc
     9361 acgatcggct tcgatgggat gatcgccatt acgccggaag gcaagctgaa tatctactcg
     9421 ggcgtcggaa acctggggac cttgtccgtc tacgataccg cgcgcgtcgc cgccgaagtg
     9481 ctcgatatgc cgtgggagaa gtgcgaagtc atctggggcg acacttcgaa gcacttgccc
     9541 tggagctcac ggcagggcgg cagccagact attcacgcta cttcgcgcgc caactatgct
     9601 gcggccatgg acgcgaagca gaaattgcag gagatcgcgg cgagagatct gggcggccgc
     9661 cccgaggatt acacgatcgg caaccagcgt gtgttccaca aaggcaaccc gggccgtggc
     9721 atgagctacg cgcaagccgc gaaacgggct atcgaactgg gcggcaaata cgacggccat
     9781 gaggtcgcca aagatctcaa cgtcatgacg aaggagtcgg cgaaggcttt ggccggcgtc
     9841 ggattgatgg gagcggccaa ggacaattac gggcgaaaag gaggaacgca agcctttgta
     9901 gccagcttcg ccgaggtcga agtggatgtt gagacgggcg tgtacaaaat tctcgactac
     9961 ctgtgtgtgg ccgactgcgg gattgtggtg aacccgcggg gccttgaggg ccaactgcac
    10021 ggcggcgcca tccagggcat tggatatacg cgcagccaga agtgggtata cgatcaggaa
    10081 tacggcgtcg ccctcgccaa gcggttccac tacaaccggc cgccgaccat tcttgatatc
    10141 ccggaaaaaa tggaatggga cgcggtgaac cttccggatc ctcaaacgcc ggtgggcgcc
    10201 aaagggatcg ccgaggcggc tgtgggagcg ggggctgcgg cagtccgctg cgcattggcc
    10261 gccgctatcg gcgacgatta tatccgccgc accccggttg gcatcgatat gattctcgct
    10321 tcgctcgagg ccaagagacg cgtcgaccgg ggactgaccg caaacgttta aagagaggct
    10381 gaaaatggca ctgattcatg atgtaatgcc agcttttgag ctgtttcagc cggtgtctat
    10441 cgacgacact ctggcgctgc tggatcgata caaaaaagag gcctgggtga tggccggcgg
    10501 tctggacact tttgactggt tgaaggatcg tatcaagcgc ccggaggtgg tggtcgatat
    10561 cagcggcgtc caggaattga agggcatcaa ggccaccgga gacggcggcg tcgaaatcgg
    10621 cgcgtccacg acgctcacag aaatcgtgcg gcacccgctg atcaaagaga aatatccgat
    10681 cctggcccag gccgcggagc ttgtggcttc cccgcagatt cgcaaccagg ggaccatcgg
    10741 cggcaacgtt tcccaggaca cgcgctgctg gtattaccgc agcggctggt cctgctaccg
    10801 cgccggcggc aatatttgct acgccgacac gccgacggcc atcaatcgcg agcacgccat
    10861 ctttgacgcc aaccgctgcg tcgcagtgaa tccgtcggat acggcgccgg cgctgatcgc
    10921 gctcgacgcc aaaatggcga ttcgctcggt gaagcgcgag cgtgtggtcg atgcggagca
    10981 tttctttatc ggccctgcca tcgacatcac gcgaatgact gtgctcgagc caggcgatct
    11041 gttgacctcg atccgtattc cggccacctg ggccggagcg cagttctact tcgaaaaagt
    11101 gcgcgagcgg caggtttggg atttcccgat cgtcaacgtg gcggcggtct ttgtggtctc
    11161 gggaggaagg atcgaccgct cccgaatcgc tgtcaatgcc gtggccgcgc acccaaagcg
    11221 gctgacgtcc gtggaggccg ccatcgccgg caagccgcgc aacgaggaga cggcaaagat
    11281 ggcaggcgaa ctggcggtgc aaggcgccca gccgctggcg cacaacgcat ataagattcc
    11341 gttgatgaga aatctcgtaa agcgggccat ccggggagag gtagcgtagc aaacatcagc
    11401 ttgagtttta tctgctcttc tatacttcga aaccggtgct gtacgttatt tcggccgccg
    11461 gtgaagtcgc gcggagactg actctgtgga gcccagggct ggaattcaaa ccggggcagc
    11521 gacagacagg tccgtctcag ttgctgatcg aattcaacaa tggttctgta gttcattcgg
    11581 gtgaacaatc aggttagtca catacgacct gttctcaggc gagcggaccg ggtattttgt
    11641 cccggacaaa gatgcgacgc gcctcgcctg ctataactgg cacggcacat ttacgtttct
    11701 aaccagtcgc aatggccgcc gcgcgatcct taccgccagc gcaaggtaga tttctagggg
    11761 gctaatccgg ggagagacgg cgtaaactcg agtcgcacag caggagattc ggccggctgc
    11821 cgcacgagcc attctggaga agggccttac aactggtcgc gatactgctc gagagctact
    11881 ttgacaagct gagcgcgggt tcgaacagct agtttatcga atagctgccg cagggaggct
    11941 ttgacagccc cctcggagat ttgcaggcgg gcgccgattt ccttgttggc caatccctga
    12001 aagatacagc ggagcaccgt cttgtcgcgg tctgtcagcg tagggcgggt tggcgccgcc
    12061 gatcggtcga cggtccggaa caacggcttc agataacttt tctccaggca gacttccccg
    12121 gcggctactt gccggacgac cgagcagagt acttccgccg agttgtgctt gtgcaaaata
    12181 ccagccactc cggcctgaat taactgcact gcttcctgat cgctgactcc ggcggtaacc
    12241 accaaaatct ggcccttgaa tcccccctgc ctggcctcgg cgacaaagtc gacggccctt
    12301 tcggccccaa ggtcaaaatc gaggagaacc atggtcgcgc cgcttttcgg caacaggctc
    12361 agcgcctgag cagcggatgc gcacttgccc acgactttca tgtccggctc tttttcgaga
    12421 atccgcgcca gtccgtcgca gaacatggcg tgatcgtcca ccaagagcag cttgatgctt
    12481 tgagttaaca aatcggtcac agctctctcc tctaaacagt ttgcagttcc acggcaaagc
    12541 aagaaccctt ttcagtgggc tcgtaacgca gttgcccgcc gtagctccgc agaatggcgc
    12601 gggagacgta aaggcccagc cccacgcctt cggctccgtc ttgcaacggc tgaaacagac
    12661 gctcgggcct ggcgattccc ggtcccgagt caacaaagcg cacaatcgcc ttcggatgct
    12721 gtacggagac gctgatgcaa agctcccgga cgtcgcaatc ctttacggcc cggaagctgt
    12781 tctgcaccag attcaggaag gcttgcagga gcccgtgggg gtctgccaaa acagtcggca
    12841 tcgattgcgg cacgcgccag cgcacaacgg catcgatttc cgtccaagcc ggctcgatga
    12901 tgatgcgaag attatccagc acttcctgga g
//
LOCUS       DASREK010000324        10472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1974822, whole genome shotgun sequence.
ACCESSION   DASREK010000324 DASREK010000000
VERSION     DASREK010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974822"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1039)
                     /locus_tag="VFA54_17675"
     CDS             complement(104..1039)
                     /locus_tag="VFA54_17675"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS52702.1"
                     /translation="MTTQRLVCPSLLLVAAAALFGQAAKNPAVKKRSAASAPAASPTR
                     RLVDAHVHYNGDRAFLDKMLARLDEHDGAAFLLTAPKDMLDVETYIAQHPGRFIGFGA
                     ISLDDPQALQQIDRFNTAGFRGLGEMTGPLKNYDDKSYWPIYERAEKYGMVILFHTGI
                     VNRMTPDVPADISVDRMRPTTLDNIARRFPKLTIIGAHLGNPDYAWAAEIARWNPNLY
                     FDLSGSTLIKLQENYQAFKSIFWWSSVVSPHTPKSNVSAFEKIVFGSDVFGGEVEEFD
                     RELERYQRMLDACGVSRQAQANIFGGTLRRILNLQ"
     gene            complement(1104..1313)
                     /locus_tag="VFA54_17680"
     CDS             complement(1104..1313)
                     /locus_tag="VFA54_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52703.1"
                     /translation="MADQYSFSHAELALLIELLERERRDLPAEIHHTDTARVRDELHR
                     RLETVDLLLKRLRGAAVETELRCGG"
     gene            complement(1371..1793)
                     /locus_tag="VFA54_17685"
     CDS             complement(1371..1793)
                     /locus_tag="VFA54_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZS52704.1"
                     /translation="MTDQEIRQLLSTAKTIAVVGLSDKPWRPSHGVSEYMQKQGYRII
                     PVNPNLSEALGEKAYASLEEVPDPIDIVNIFRRSEAVEPVVNSAIRIGARAVWMQEGV
                     VHREAAERARRAGIAVIMDRCILKEHRKRFRMAADRLA"
     gene            complement(1893..2651)
                     /locus_tag="VFA54_17690"
     CDS             complement(1893..2651)
                     /locus_tag="VFA54_17690"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZS52705.1"
                     /translation="MQERLQKIISAAGITSRRKAEELIQAGRVTVNGRVVTELGSKAD
                     PEKDHIKVDGKLLRRSRDLVYLMLNKPRNCVTTVSDPEGRDTVMQFLKGVRERVYPVG
                     RLDFASEGLLLFTNDGEFANAILTGGSRVPKTYLVKINGNPTEEALRQFREGITIEGR
                     KTAPARIRNVRPGENPWFEVTLIEGRQNQIRRMFRKLGFLVEKLKRVRIGFLELGPLR
                     PGQFRYLTDEEVRRFRRIAAARNQREQSMRRMKI"
     gene            complement(2753..3490)
                     /gene="scpB"
                     /locus_tag="VFA54_17695"
     CDS             complement(2753..3490)
                     /gene="scpB"
                     /locus_tag="VFA54_17695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZS52706.1"
                     /translation="MSERRALLEAILYITDDPLTVEQLAKGLEWPKEEVEALIAELVE
                     SHRQADRGVEVRAIAGGYKISTKPEHHEAVRRFVKTLKPPMKLSIPALETLSVIAYKQ
                     PVTLPEIQEIRGVGAAGVIKTLLDRKLITTSGRKQVIGRPILYKTTREFLVQFGLNDL
                     SELPSLSEFEQLSRAALGDGVDPAAQAAAGEPPLPQAATEQPSPEPTASEPVSETSGV
                     PDGQLGDTAGTAEEHPAAVQPSEDDLR"
     gene            complement(3483..4262)
                     /locus_tag="VFA54_17700"
     CDS             complement(3483..4262)
                     /locus_tag="VFA54_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZS52707.1"
                     /translation="MDASPLNVHLEQYEGPLDLLLDLIRRQQIDIYNIPIARITSQYL
                     DYLHRAESLNVDLGGEFVLMAATLIQIKSRMLLPAEPVMPGEQAEDPRAELVNRLLEH
                     EKYKNAAQMLHQKQLIEENVWSNPQIGAFLDDDDEPGLAVTLIDLVKTFEQILERAKK
                     RPQFEVAGEEVSVAQQIQYLKNILLASDEPLALQTIFEKQVGRRPLVALFLAVLELVR
                     MQAILLRQKETFGEIFIRKHKMFNVIFQNEQPLMAVDEDYV"
     gene            complement(4268..4966)
                     /locus_tag="VFA54_17705"
     CDS             complement(4268..4966)
                     /locus_tag="VFA54_17705"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZS52708.1"
                     /translation="MAFAVTETTLFFLFSFIAFLFTISVHESAHALAADRCGDATARL
                     AGRISLNPLRHIELFGTIILPLITALSGMPTFGWAKPTPVNPAKLRKPRRDDILVSAA
                     GPVSNLLTALACLFFLEAIRASSAAGAQVVNDLAWTGTPGIGSSVLNPVAWLLHRLLE
                     ISVVLGVFNLFPVPPLDGSHILQQLLPWRARVVYRSLSRYGMLVLLLVLWYTPLSQWA
                     FDRPMRLVNSLLRM"
     gene            5261..5950
                     /locus_tag="VFA54_17710"
     CDS             5261..5950
                     /locus_tag="VFA54_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HZS52709.1"
                     /translation="MKLHIGIAAIALAGLLSAANKEEPKEVQRLNEAATVFDEIMGIK
                     ERTIPQELLDKAECVIIVPGLKSAAFIFGGKYGKGVISCRPENGQGWTGPSTVRIEGG
                     SIGFQLGGKETDYVMLVMNRRGVEKLLQSKFTLGGDMSVAGGPVGREADAQTDAQMHA
                     EMLAYSRSRGLFAGVSLNGATLRQDQSDNRVIYGKPVTPRQILLEGLQPPASAQKLIA
                     TLNKYSSRRKS"
     gene            complement(5998..8268)
                     /locus_tag="VFA54_17715"
     CDS             complement(5998..8268)
                     /locus_tag="VFA54_17715"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HZS52710.1"
                     /translation="MAYFVLLAIAFWTFFVLPLRAGPPDGENGHRRKVRAYRVDKPPT
                     LDGLVNEDVWKRMEPAANFIQQQPDEGLPATEQTEVRIGFDDNNLYIGIICFDSEPEN
                     IVVTQNRRDAPLTDTDSVQILLDTFKDGQNAFVFGTSPTGIEYDAQVSKAGQSRQGVG
                     QPARAGGQGGFGASNQSAGAAAFNLNWDAVWKVRSRITERGWESEMVIPFKTLRYRPG
                     SGDVWGLNITRNLRRRNEQSFWSPLSRNFTLNQVEMAGDLEGLETKVHRNLKLTPYIL
                     GGYKQDYTVTPEVSKGVHDVGLDMKYSLTPSLTLDATFNTDFAQVEVDEQQINLTRFD
                     LFFPEKRPFFLENSGNFDFGTPMETEIFFSRRIGLTAAGDQVPIDAGVRLTGKAGHYD
                     LGFLNMKTREVEGITPGNDFTVLRVSREFANRSSLGVIGVNRQSTSHSGLVEPFNRTA
                     GVDGNIGFGRYANLFSYVAKTQTPGLSGDDMTGSSAFEYDDEHHRIDLGYNQVGRNFN
                     PEVGFVRRVGYRKPFFGYRYTYYPEGEHLRSIMPHFQWNRWYTLGTNDEESGFEHYHL
                     DTRWQNGGSLGLAFNRNFERLDAPFAVFPNVNIAPGRYRYNEAVLNFGTDQTARFFTL
                     SNLSIGHFYSGTIKTINFSGGIRRGQNLTWTGSYLRNFISLPAGKFNTDLIGFRFNWA
                     FTPKSYFQSFIQYNSEASQVGVNLRVALLSTSSNGFFLVYNTRASTDDFLDPHDVIRT
                     TQSRALFFKYTYLFDF"
     gene            complement(8598..9455)
                     /locus_tag="VFA54_17720"
     CDS             complement(8598..9455)
                     /locus_tag="VFA54_17720"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZS52711.1"
                     /translation="MTKWNRSLAFYLAAAAVSAQEVPPQVPILTAVYTRAAYHTARRT
                     TERDPFAAVAGSCAIAPSDSSFTLNCQSPSPPEPRPGRRYYYSIVLFQDLNDAVYLAA
                     CYAGARDSRCGDLKEGQTFSAEVQDRNIRIVMRGEQLPLRILETRPRPASIDSPTSGT
                     PSEVRPSAGTPSRAPYSNVPFTAGTPSDVRPSDVPPSPGSPSNVPLSQVSPSVTSAFG
                     ARLYVYCDNGAAQVSVDGQVVGRPPLEIPLPPGRHTVMVQAQGYPRWIRQVDVPAGQT
                     TRIAADLRH"
     gene            9543..10202
                     /locus_tag="VFA54_17725"
     CDS             9543..10202
                     /locus_tag="VFA54_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52712.1"
                     /translation="MQFGGILPGLRNGDKAFLIALLVIGAALLAYAEYRRILYAAIDD
                     RYGDWVRFHTRAGQLPERGRRLQYRLEYSLASFFAYGLSDFFLLLRLLLWDLFVVFAL
                     LSPFWHSLVFSDWNPFAAMAVSAAGTGAYTVWKLRKSLGLCRVHAWSAETWRCERCGE
                     FHADAEERLEAKNPGKTALPKTSLPSRRRFSWHEFWENRLQMLVDRLQRELPSSPPPA
                     G"
     gene            complement(10214..>10472)
                     /locus_tag="VFA54_17730"
     CDS             complement(10214..>10472)
                     /locus_tag="VFA54_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZS52713.1"
                     /translation="IFFGRFLHFAVMPIFEYRCATCNHAFEELILTRNQEHVECPNCG
                     GSAVEPLVSRFAAQTGNKFSNFGECASRAAGICEAGGGTMT"
BASE COUNT         2073 a         3124 c         3195 g         2080 t
ORIGIN      
        1 gccggcgccg gaatcattct tccgctcaca gggctcatga aagaaacacg cggcgagggc
       61 tgcgcggcgt tgcagcactc gaaatccacg cgctgccggg cggctactgg agattcagga
      121 tgcgccgcag ggttcccccg aagatattgg cctgcgcctg ccgcgagacg ccgcacgcgt
      181 cgagcatcct ttggtaccgc tcgagttcgc ggtcaaattc ctcgacctcc ccgccaaaca
      241 cgtcggagcc gaagacaatt ttttcgaagg cgctgacatt cgacttcggc gtgtgaggac
      301 tcacgacgct ggaccaccag aagatggatt taaatgcctg atagttttcc tgaagcttga
      361 tcagcgtgga gccggaaaga tcaaagtaaa gattgggatt ccaccgcgcg atctcggcgg
      421 cccaggcata atccggatta cccaggtgcg caccgatgat ggtgagcttg ggaaagcggc
      481 gggcgatatt gtcaagagtc gtcggccgca tccggtccac gctgatgtcg gccggcacat
      541 ccggagtcat gcggttcacg atgccggtat gaaatagaat caccatccca tatttctcgg
      601 cgcgctcata aatcggccag taactcttgt cgtcgtagtt cttgagcgga ccggtcatct
      661 cgcccagccc gcggaaaccg gcggtgttga accggtcgat ctgctgaaga gcctgcgggt
      721 catcgaggct gattgcgcca aaaccgataa agcggccggg atgctgggcg atgtaagtct
      781 cgacgtccag catgtccttg ggcgcggtga ggagaaaggc ggcgccgtcg tgctcgtcca
      841 gccgcgccag cattttgtcg aggaaggcgc gatcgccgtt gtaatggacg tgagcgtcca
      901 ccagacgccg cgtcggactg gcggctggcg ccgaggccgc gcttctcttt ttcaccgccg
      961 ggtttttcgc cgcctggccg aacagagctg cggcagcaac gagcagaaga gaaggacaaa
     1021 ccagccgctg tgtcgtcatg tgtccacccc gctgatatga atctagcaga cccggccgcg
     1081 tcctgcggcc tgcgccggcg gcgctacccg ccgcagcgaa gctctgtctc cacggctgcg
     1141 ccccgcaagc gcttcagcaa gagatcgacc gtttcgaggc gccggtgcag ctcatcgcga
     1201 acccgggccg tatcggtgtg gtggatttcg gccgggagat cccgcctctc gcgttcgagc
     1261 agttcgatga ggagcgctaa ttccgcatgg ctgaatgagt actgatccgc catttttcgc
     1321 ctccggaagc tctctgtttt tcaatgtagc attctcttcg cacagcgggg ctaggccagc
     1381 cggtccgccg ccatgcgaaa acgcttgcgg tgctccttca aaatgcagcg gtccatgatg
     1441 acggcgattc cggccctcct cgctctttcc gccgcttcgc ggtggaccac gccttcctgc
     1501 atccacacgg cccgcgcgcc tatccggatg gcggagttta ccaccggctc caccgcctcc
     1561 gagcgccgga aaatatttac gatatcgatt ggatccggca cttcttcgag gcttgcataa
     1621 gccttctccc ccaaggcttc ggagagattc gggttcacgg gaataatgcg gtagccttgt
     1681 ttctgcatgt attcagaaac gccgtggctc ggacgccacg gcttatctga gaggcccacc
     1741 acggcgatcg tcttggccgt gcttaacaac tgccggatct cttggtccgt catctcaata
     1801 tgtctctccg gaatgtctgc gccatcgacc agtccgggag tcgagccggc gccgggccca
     1861 gcccagggca ttcacaggaa gagcctcata gctcatatct tcattctacg catgctttgc
     1921 tcgcgctgat tccttgcggc ggcgatccgc cggaatctcc ggacctcctc atcggtgaga
     1981 taccggaatt gacccgggcg cagcggcccc agttccagaa aaccgatccg cacgcgtttc
     2041 agcttttcca ccaggaagcc cagtttccgg aacatgcggc gtatttggtt ttggcggccc
     2101 tcgataagcg tcacttcgaa ccaggggttc tcgcccggcc gcacgttgcg gatccgcgcc
     2161 ggcgcggtct tgcgcccctc gattgttatg ccttcgcgaa attggcgcag cgcctcctcc
     2221 gtcgggttgc cgttgatctt gaccaaatag gtcttaggca ctcgagaacc gccggtgaga
     2281 atggcgttgg caaactcgcc gtcgttggtg aacagcagca acccttcgct ggcgaaatcc
     2341 aaccgcccca cgggataaac ccgctcccgc acgcctttga gaaactgcat caccgtgtcg
     2401 cggccctccg ggtcggaaac cgtggtgacg caattcctgg gcttattcag catgagataa
     2461 acgaggtcgc gcgagcgccg cagcagcttg ccgtccactt tgatgtggtc tttctcggga
     2521 tccgctttgc tgcccagttc cgtcacaacc cggccgttga cggtgacgcg ccccgcctga
     2581 atcagctctt cggctttgcg ccgggatgta atgccggcgg cggaaatgat tttttgaagg
     2641 cgctcttgca tggacgtgta agccgccgag gaaaacgcgg gcgcccgggc cggctccggt
     2701 tatgccgatg tctccagcga ccccagcgcc cggcggcgcc gcttccgcag tttcaccgga
     2761 gatcgtcctc agatggctgt actgcggccg ggtgctcttc cgctgtcccg gcggtgtctc
     2821 ccagttgccc gtcaggaacg ccggaggttt ccgaaaccgg ctcactggct gttggctcgg
     2881 gcgatggttg ttccgtcgca gcttgtggca gcggcggctc gccggccgcc gcttgtgccg
     2941 cggggtccac gccgtcaccg agcgccgcgc ggcttaactg ctcaaattca ctgagactgg
     3001 gcagttcact gaggtcgttg aggccgaact gcaccagaaa ctcccgcgtc gtcttgtaga
     3061 gaatgggccg tccgatcacc tgcttccgcc ccgaggtggt gatcagcttc cggtccagca
     3121 gcgttttgat caccccggcg gcgcccacgc cccggatctc ctgaatctcc ggaagggtca
     3181 ccggctgctt gtaggcgatc acggaaagcg tctcgagagc ggggatggaa agtttcatgg
     3241 gcggcttgag cgtcttgaca aagcgccgaa cggcctcgtg atgttccggc ttggtggaga
     3301 ttttgtaacc gcccgcgatc gcacggacct ccacgccgcg gtccgcctgc cggtggctct
     3361 cgaccagctc ggcgatgagc gcctccactt cttccttggg ccattccagg cccttggcca
     3421 actgctccac cgtgagcgga tcgtcggtaa tgtacagaat cgcctccaga agcgcccggc
     3481 gttcagacat aatcctcgtc cacggccatc aggggctgct cgttctggaa tatgacgtta
     3541 aacattttat gttttcgaat gaaaatctcc ccgaaagttt ccttctggcg gagcaaaatg
     3601 gcctgcatcc tcaccagttc gagcacggca agaaagaggg ccactagggg ccgccggccc
     3661 acttgctttt cgaaaatagt ctggagcgcc agcggttcgt cgcttgccag caggatgttt
     3721 ttcaaatact ggatctgctg ggctacggag acttcctcac cggccacctc gaactgagga
     3781 cgtttcttgg cccgctccaa aatctgctcg aaggttttca ccagatcgat cagcgtcaca
     3841 gccaggccgg gttcatcatc gtcgtccaaa aaggccccaa tctggggatt cgaccagaca
     3901 ttttcctcga tcaattgctt ttggtggagc atttgggccg cgttcttgta tttttcgtgc
     3961 tccagcagcc ggttcacgag ctcggcccgc ggatcctcgg cctgctcgcc gggcatcacg
     4021 ggctcggcgg gcagcagcat cctcgatttg atctgaatca aagtcgccgc catgaggacg
     4081 aactcgcctc ccagatcgac gttgagagat tccgcccggt gcaggtagtc gagatactgg
     4141 gaagtgatcc gggcaatagg gatattgtaa atgtcgatct gctgccggcg gatcaggtcc
     4201 agcagaaggt ccaggggccc ttcgtactgt tccaagtgca cgttgagcgg cgaggcgtcc
     4261 actggcgtta cattctcaaa agggaattga ccagccgcat ggggcgatca aacgcccatt
     4321 gactcagcgg cgtataccaa agcaccagca gaagcaccag cattccgtag cggctgaggc
     4381 tccggtacac cacccgcgcc cgccacggca acaactgctg caaaatatgg ctgccatcaa
     4441 gcggcggcac tggaaacaga ttgaatacgc caagaacgac gctgatctcg agaagccggt
     4501 gcaacagcca ggccacggga ttcagcaccg aagagccgat gccgggcgtg ccggtccaag
     4561 ccagatcatt caccacctgc gctccggcgg ccgaactggc gcgaattgct tccaagaaga
     4621 acaggcacgc gagagccgtc aacagattgc tcacgggacc tgcggccgaa accaggatgt
     4681 cgtcgcggcg gggttttctg agttttgccg ggttcaccgg cgtgggcttg gcccatccga
     4741 aagtcggcat cccgctgagc gccgttatca gtggcaggat gatcgtgcca aaaagctcga
     4801 tgtgccgcag cgggtttagt gaaatacggc ccgccagacg cgctgtggcg tcgccgcatc
     4861 gatcggctgc cagggcgtgc gccgactcat gcacgctgat ggtgaacagg aaggcgataa
     4921 agctgaacag gaaaaacagc gtcgtttccg tcacggcaaa agccatctta gcaggtgtgg
     4981 agatctcgcc gcgcgaacgc cgcggatagg aaaagctgcg cccttggttt tgccgccgcg
     5041 cagcgatttc gcggcttccc gaaagccgtt cccttcttct cgatcttccg gcggtccaac
     5101 ggcgtcacgc aggccatggc cgcttgtggt aaaaataaca ttggattgca acgcgtggct
     5161 tctggccctg gcgcttccgg tgggaaaaac cgctggcctg cggcgttcgt cgtttccgtg
     5221 aactccggct gggccggcgg ggtggaaaag aggaggattc atgaaactgc atatcggaat
     5281 tgcggcaata gccctggctg gcctgctcag cgccgcgaac aaagaggagc cgaaggaggt
     5341 tcagcgcctg aacgaggcgg cgaccgtctt tgacgagatc atgggcatca aagaacgcac
     5401 aattccccag gagctgctgg acaaagcgga atgcgtgatc atcgtgccag ggttgaagag
     5461 cgccgctttt atcttcgggg gcaagtacgg caaaggagtg atcagttgcc gtccggaaaa
     5521 cggccaggga tggacagggc catcgaccgt ccggattgag ggcggcagca tcggcttcca
     5581 gttgggcggc aaagagaccg actacgtcat gctggtgatg aacaggcgcg gcgtggagaa
     5641 gctgctccaa agtaagttca cgctcggcgg ggacatgagc gttgccggcg ggccggtcgg
     5701 ccgcgaagcc gatgcgcaaa ctgacgctca gatgcacgcc gaaatgctgg cctactcccg
     5761 ttcccgcggt ttgtttgccg gagtgtctct caacggcgcc accttgcggc aggatcaaag
     5821 cgacaatcgc gtgatctacg gcaagccggt aacgccgcgc cagatcctcc tggagggctt
     5881 gcagcccccg gcttccgcgc aaaagctgat cgccactctc aataagtatt cttcacgaag
     5941 aaagtcgtag tagccgcctc acgaatcggg agccgcttcc ggctcccttc ctctccgcta
     6001 aaaatcgaaa agataggtgt atttgaagaa cagcgcccgg ctctgggtcg tgcgaatcac
     6061 atcgtgcgga tcgagaaaat cgtcggtcga ggcgcgtgta ttgtaaacca gaaaaaatcc
     6121 gttgctcgac gtggaaagaa gagcaacccg gagattgact cctacctggc tggcttcgct
     6181 gttgtactgg atgaaggatt ggaaatagct cttgggcgtg aacgcccagt tgaagcggaa
     6241 cccgatcaga tcggtattaa acttgcctgc cggcaggctg atgaagtttc gcaggtaact
     6301 gcccgtccag gtgaggttct ggccccgccg gatgccgccg ctgaaattga tggttttgat
     6361 cgtgccgctg taaaaatggc cgatggaaag gttgctcaag gtgaagaatc tggctgtctg
     6421 gtcggtgccg aagttcagaa cggcttcgtt gtagcggtaa cggccggggg cgatattcac
     6481 gttcggaaac acggcgaagg gcgcgtccaa ccgctcgaaa ttgcgattga aggctagtcc
     6541 caggctgccg ccgttctgcc accgcgtatc gagatggtaa tgctcgaagc cggattcttc
     6601 gtcattggtt cccagcgtgt accagcgatt ccactgaaaa tgcggcatga tgctccgcag
     6661 atgctcgccc tccgggtagt acgtatatcg gtagccgaaa aaaggcttgc ggtagccgac
     6721 ccggcgcacg aagccgacct cggggttgaa gttacgcccc acctggttgt atccgagatc
     6781 gatccggtga tgctcgtcat cgtactcgaa agccgatgag ccggtcatgt cgtcgccgga
     6841 aaggcctggc gtctgggtct tggccacata gctgaacagg ttcgcgtagc ggccgaagcc
     6901 gatattccca tccacgccgg cagtgcggtt aaagggctcc accaaacccg aatgagacgt
     6961 cgattggcga ttcacgccga tcacgccgag cgaagatcgg ttggcaaatt cgcggctcac
     7021 ccgcaaaacc gtgaagtcgt tcccgggggt gatgccttcc acctcgcgtg tcttcatatt
     7081 gagaaagcct aggtcgtaat ggcccgcttt gccggtcagg cgcacgcccg catcaatagg
     7141 cacctggtct cccgcggcgg tcaagccaat ccggcgggag aagaaaattt cggtctccat
     7201 gggcgtgccg aaatcaaagt ttcccgagtt ctccaggaag aacgggcgtt tctcgggaaa
     7261 gaacaaatcg aagcgcgtga gattgatttg ctgctcatcg acttcgacct gcgcgaagtc
     7321 ggtgttgaac gtggcgtcta gcgtgagcga gggcgtaagg ctgtacttca tgtccaggcc
     7381 cacgtcatgc acgcccttgg agacctccgg agtgacggtg taatcctgct tgtagccgcc
     7441 caggatgtac ggagtcagct ttagattccg gtggaccttg gtttccaggc cctcgagatc
     7501 tccggccatt tcgacctgat tcagcgtgaa gttgcgcgaa aggggcgacc agaacgattg
     7561 ctcgttgcgc cggcggaggt tgcgcgtgat atttaagccc cacacgtcgc cggaccctgg
     7621 ccggtaccgc agggttttga aaggtatgac catctcgctt tcccagccgc gctcggtgat
     7681 ccgcgaccgg actttccaga cggcatccca attcaggtta aacgccgcag cccccgcgct
     7741 ttgattggag gccccaaagc cgccctgccc gccggcgcgc gccggttggc ccaccccctg
     7801 gcggctctgc cccgctttcg aaacctgggc gtcgtactcg attccagtcg gactggtgcc
     7861 gaaaacaaac gcattttgtc cgtctttaaa ggtatcgagc aagatctgaa cggaatcggt
     7921 gtcagtcaga ggagcgtcgc gccggttctg ggtcaccacg atattctctg gttcggagtc
     7981 aaagcaaata atgccgatgt aaagattgtt atcgtcaaag ccaatgcgga cttctgtctg
     8041 ctcggtcgcg ggcaggccct cgtccggctg ttgttgaatg aaattggcgg cgggctccat
     8101 ccgcttccaa acatcttcat tgaccagccc gtccagcgtg ggcggtttat ccacacgata
     8161 ggcacggacc tttcgccggt gaccgttttc gccatccgga ggcccagccc gcaagggcaa
     8221 cacgaagaaa gtccagaatg cgatcgctag aaggacgaaa taggccatgt aagctgaccg
     8281 cgcagacgcc atctgttgag gcgtcacgcg ggcgccagac agggctgagc cgcagtcgtt
     8341 gtcgagcaag gcgtcgcggc aggcggtttg tccccgtcaa cctcgccgga cgcagtagaa
     8401 agcttttaaa taacatgcgg tagggcgaaa cggtgtaaat aaattatgaa ggccggccag
     8461 ttgcgcgagg aggggcgcct attgcggcgt ggagtcaata tcagtttccg cagaaatttt
     8521 gcggcgttgc cgaccggctc gtgcatccgt ttgcgccgcg aaaccttgcc cgggcgctgc
     8581 gcctgcaaac agcggcctca atgccgcaaa tcggcggcga tgcgcgtggt ctggccggca
     8641 ggaacatcga cctggcggat ccagcgtgga tagccctgag cctgcaccat cacggtgtga
     8701 cgccccggcg gcaagggaat ctcgagcggc gggcgcccga caacctggcc gtccacggag
     8761 acctgcgccg ccccgttatc gcaatagaca tacagccggg ctccgaaggc agaggttacg
     8821 gaaggcgaca cctgggaaag cggcacattg gagggagacc ccggggatgg cggaacatcg
     8881 gaagggcgga cgtcggaagg ggttcccgcc gtaaagggaa cgttcgagta gggcgcacgc
     8941 gacggagtac ccgccgatgg gcgaacctcg gacggcgtgc cggaggtggg cgaatcaatg
     9001 ctcgccgggc gcgggcgcgt ttccaaaatc cgcaggggca actgctcgcc ccgcatcaca
     9061 atacgaatat tccggtcttg cacttcggcc gagaaggtct gtccttcttt gagatcgccg
     9121 cagcggctgt cgcgcgcgcc ggcatagcag gccgccagat acacggcgtc attgagatcc
     9181 tggaaaagga caatggagta gtagtaacgc cggcccggtc gaggctcggg cggagacggg
     9241 ctttggcagt tcagcgtgaa cgagctgtcg gaaggagcaa ttgcgcagga cccggccacc
     9301 gcggcgaacg ggtcccgttc ggtcgtcctg cgcgccgtat ggtaggctgc gcgagtatac
     9361 actgcggtca aaatcggaac ctgagggggg acttcctgcg cgcttactgc cgcggccgcc
     9421 aggtaaaaag ccaggcttcg gttccatttg gtcatactgc gttccaagct gcgctgctcc
     9481 gggcgctgcc cacatccgat tgacggcaat cgtcccgaag ccgtgcagcc gcggcggata
     9541 taatgcagtt cgggggcata ttgccgggct taaggaacgg ggacaaggca tttctcatcg
     9601 cgctgctggt cattggggcg gcgctgcttg cctacgccga gtatcgccgc atcttatatg
     9661 ccgcgataga tgaccggtac ggtgattggg ttcgctttca cacgcgggcg ggccaactgc
     9721 cggaacgtgg ccgccgtctc caatacaggc tggaatacag cctggcaagc tttttcgcct
     9781 acggtttgag cgattttttt ctcctgctga ggctcctgtt gtgggatttg tttgtcgtat
     9841 tcgctctact gtcgccgttc tggcatagtc tcgtattttc agactggaat ccgttcgcag
     9901 ccatggcggt atccgccgcc ggcacagggg cctacaccgt ctggaagctg cggaaaagtc
     9961 tcgggctgtg ccgcgtgcac gcttggtcgg ccgaaacctg gcgttgcgaa cgatgcgggg
    10021 agttccacgc cgacgcagaa gagcgcctgg aagccaaaaa ccccggaaaa acggcgctgc
    10081 ccaaaacgag cttgccctcc cggcgcaggt tttcctggca cgaattctgg gagaaccggc
    10141 tgcaaatgct cgtggatcgg ctgcaaagag aattgccttc cagcccgccg ccggcgggct
    10201 agcgccggat ccctcaagtc atggtcccgc cgccagcctc acagatccca gccgcgcggc
    10261 tggcgcactc tccaaaattc gagaacttat tcccggtctg ggcagcaaag cgcgaaacca
    10321 gcggctccac ggcagaccca ccgcaattcg ggcattccac gtgctcttga ttccgggtga
    10381 ggatcagctc ttcgaaagcg tggttgcacg ttgcgcagcg atactcgaag atcggcataa
    10441 cggcaaaatg gagaaatctt ccaaaaaaga ta
//
LOCUS       DASREK010000325         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1639564, whole genome shotgun sequence.
ACCESSION   DASREK010000325 DASREK010000000
VERSION     DASREK010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639564"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFA54_17735"
     CDS             complement(<1..297)
                     /locus_tag="VFA54_17735"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZS52714.1"
                     /translation="MRFVLITILISAALLAQSPTAAIVGLVTDQSDSAIPGAQVTGRN
                     LQTNYVQTFTTGAEGLYRFPALPLGTYEVSVTAKGFERFVQSNVVIRGAEVVRVD"
     gene            complement(332..2728)
                     /locus_tag="VFA54_17740"
     CDS             complement(332..2728)
                     /locus_tag="VFA54_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52715.1"
                     /translation="MDRRTFLFSVPAAGALKPAAAAPAGAAVSIALAGSRHARVHVAV
                     RELEQQLRAAGYAVDLAEGGPRIAGHRQIRLAVHKNRVAEAFEISSAADGISILAGGD
                     RGLVYGISELSRQVAAQGRLPVGISLSRAPRFAVRRWSSSVSHLMGAPWDERTMLLER
                     FAFIRSEILPRAGAYGMNSVEINGRPGDGWDVAWMIAFEKYPELSRLYPEGERNRRMA
                     MVEEVGRAAHENLLDLLVWSHELYLPPGFAELYPQVRGTEYPVCLSHEFLGRFIRDKY
                     IEFFERVPSVDGIVLSVNESGQFSLITDAGCKCDRCAPMSQHEKLMAVLQHVIAVCER
                     LKKQLVLRTFQSSFIHDLDGHPELETIRKAYTGLPASVYIMSKYCPLDFYGGEIADEP
                     LIGVFPNPHLVEFSLDVEWQGRTFVPVLTPGNFRRRIRHALDRKCAGIVARVDFPFPS
                     MEPGPIFGHPNEFNAVFMGELLWADSDPSESAVRWASARYGREGAQQIAAALLSTEEI
                     TQKTFFAQGQTLINYHNMIASVSNADSNLWSHALSKWDSSKRELSRSFFEPADELIER
                     CREEKRRAASLAQESLRVVERANSLTPPDRERLRSDFEKLRDTALLWGFLSELYLLAR
                     QAGSQFARLRGTAASALLTAVRMEGRYGRNSWPVVSPDRGVTAYEFVQQILRKYIGGF
                     TGEPVRDEVKTHYGDSMVTVPIVRPAATEVLWRRLVECCRPGFAYGSTAAVRLDWPEK
                     LADIRTQEQDLIFRSRTGQELRIPLPLPVQAALFQEAPKLIFRVVRTTRELVVTAERL
                     "
     gene            complement(2774..>3033)
                     /locus_tag="VFA54_17745"
     CDS             complement(2774..>3033)
                     /locus_tag="VFA54_17745"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HZS52716.1"
                     /translation="FHAALWGAAGNRALLETLDRLVPKLFALGLIRHTNPARGAMRRI
                     AAEHALLLEAISKRESGRSQELLKRSLAQGQAEDAELTADP"
BASE COUNT          606 a          965 c          879 g          583 t
ORIGIN      
        1 gtcaacgcgg accacttccg cgcctcgtat cactacattc gactgaacga accgctcgaa
       61 gcctttcgct gtgacgctca cctcgtacgt tcccaacggc agtgccggaa accggtagag
      121 tccttcggct ccggtggtga acgtctggac ataattggtt tgcaggttcc ggcctgtcac
      181 ctgggcgccc gggatggcgc tatcggactg gtccgtgacg agcccgacaa ttgcggcagt
      241 tggcgactgt gccagcaagg ccgcagaaat taagattgta atcaatacga atcgcataac
      301 tactcctttc gacgagaatg ccgactcgat actacaaacg ttccgcggtc acgaccagct
      361 cacgcgtggt cctgaccacc cgaaaaatca gcttcggcgc ctcctgaaat agagcagctt
      421 gaacaggaag cggcagcgga atccggagtt cctgcccagt cctgctcctg aaaatgaggt
      481 cttgttcttg cgtccttatg tccgccagtt tctccggcca atccaggcga accgccgcag
      541 tactaccgta cgcaaaaccg ggccggcagc actctaccag gcggcgccag aggacttctg
      601 tggccgctgg ccgaacgatg ggcactgtta ccatgctgtc gccataatgc gtcttcacct
      661 cgtctcgaac cggctctccg gtaaacccgc caatatactt acgcaaaatc tgctgcacga
      721 actcataggc ggtgacaccg cggtccggcg acaccacagg ccaactgttg cggccatagc
      781 gcccctccat ccgtaccgcg gttagcaggg ccgacgccgc tgtcccgcgc aggcgcgcga
      841 attgggagcc ggcctgccgg gcgagcaggt atagttcgga caggaagccc cacagcaggg
      901 cggtatcgcg cagtttttcg aagtcggagc gcaagcgttc gcggtccgga ggcgttaggg
      961 aattcgcccg ctcgacaaca cgaagcgatt cctgggcaag tgacgcggcc cgccgttttt
     1021 cttcccggca gcgttcgatc agctcgtcag caggctcgaa aaacgaccgt gacagctcac
     1081 gcttggagga gtcccacttg ctcagcgcgt ggctccagag attgctgtct gcgttggaca
     1141 cggaggcgat catgttgtgg taattgatca aagtctgccc ttgggcgaag aatgtttttt
     1201 gcgtaatctc ctctgtcgac aacagggcag ccgcaatctg ctgcgcgcct tcgcggccgt
     1261 atcgtgcgct cgcccaacgc acggccgact cgctgggatc cgaatccgcc cataacagct
     1321 cgcccatgaa gacggcattg aactcgttgg gatgtccgaa aatcgggccg ggctccatgg
     1381 aggggaacgg gaagtctacg cgcgccacga tgccggcgca ttttctgtcc agtgcgtgcc
     1441 tgatacgccg ccggaaatta ccgggcgtca gcacaggcac gaacgtgcgc ccctgccact
     1501 cgacgtcgag gctgaattct accaggtgag ggttcgggaa cacgccgatc agaggctcgt
     1561 ccgcgatctc gccgccgtag aaatcaagcg ggcagtattt cgacatgata tacacactcg
     1621 ccggcaggcc cgtgtacgcc ttgcgaatgg tctccaactc cggatgaccg tcgagatcgt
     1681 ggataaagga gctttggaac gtacgcagga ccaactgctt cttgaggcgc tcgcacaccg
     1741 caatcacgtg ttggagcacc gccatgagtt tttcatgttg gctcatgggc gcgcagcggt
     1801 cgcatttgca gccggcgtcc gtgatcaggc tgaactgtcc tgactcgttc accgacagta
     1861 cgatgccatc caccgacggc acacgttcaa aaaattcgat gtatttgtcg cggatgaacc
     1921 ggcccaggaa ttcatgggag aggcaaaccg gatactcggt gccgcgcacc tgtggataca
     1981 gctctgcaaa gccgggcggc aaatagagtt cgtggctcca gaccagcagg tccaacaaat
     2041 tttcgtgtgc ggcgcgtccg acttcttcca ccatcgccat acggcggttg cgctcgcctt
     2101 ccgggtagag gcggctcaat tccgggtact tttcgaaggc gatcatccag gcgacatccc
     2161 agccgtcacc gggacggccg ttgatctcga cggaattcat tccgtaggca ccggcgcggg
     2221 gcagtatctc cgatcggatg aaagcgaagc gctccagcag catcgtccgc tcatcccagg
     2281 gagcgcccat gagatgactt actgaggagg accagcggcg taccgcaaaa cgcggcgcac
     2341 ggctcagcga gattccgacc ggcagccggc cctgcgcggc gacctgacgc gaaagttcgg
     2401 agattccgta aaccaggccg cgatcgcccc ccgccaggat cgaaattcca tcagcggcgc
     2461 tgctgatctc gaatgcctcg gcgaccctgt ttttgtgaac ggccaggcgg atctgccggt
     2521 gaccggcaat acgcggtccg ccttccgcga gatctacggc gtatccggca gcgcggagtt
     2581 gttgctcgag ttctcgcaca gccacgtgta cgcgggcgtg ccgcgacccc gccaatgcga
     2641 tcgacactgc cgctccggcg ggcgccgcag cggccggttt cagggcgccc gccgcaggca
     2701 ctgaaaagag aaacgtccgt cgatccatct tttaccgccg atctctttcc ctgccgatct
     2761 atttcaccgc ccatcaaggg tcagcagtta attccgcgtc ctccgcctga ccctgcgcca
     2821 gcgaccgctt cagcagttcc tgagaccttc cgctttcgcg cttgctaatt gcttccaata
     2881 agagagcgtg ttccgccgcg atccgccgca tggcgccacg ggcaggattt gtgtggcgga
     2941 tcaagcccag tgcgaatagc ttcggcacca gccggtccag agtctctaac agtgcgcgat
     3001 taccagccgc tccccacaac gccgcgtgga agt
//
LOCUS       DASREK010000326         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_651768, whole genome shotgun sequence.
ACCESSION   DASREK010000326 DASREK010000000
VERSION     DASREK010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651768"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(973..2325)
                     /gene="tssK"
                     /locus_tag="VFA54_17750"
     CDS             complement(973..2325)
                     /gene="tssK"
                     /locus_tag="VFA54_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HZS52717.1"
                     /translation="MKPLQPVIWTKGTFLTPQHLQAQDRFLENLLQFHLENLSFRPTG
                     FRSLRISQEALSAGALGISNASGILPDGLLFDIPDSDNVPAPKQLADCFEADQTTVDL
                     FLAIPHYRERGVNIANATGSGARFRAEVALVRDENTGQSEKPVQLARRNLRLLSETEA
                     HEGFSILRVARVRRSETGTFQLDPRFVPPLLDFRASEYLVSIARRLIEILIARSGAIA
                     GTRRQRNQSLADFTAADIANFWLLYTINTALPAFRHLFETRGGPPEALYSAMTTLAGA
                     LTTFSTEIHPRDLPAYNHDDLGGCFTELDEKLRALLEAVVPSNFVSLPLKPVQPAIYA
                     TSIDNEKYLVNTRMYLAVSAGASVAEIVAKTPQLVKVCSASHIEHLVRQALPGVTLTY
                     AAAPPSAIPVKLNYHYFSLNQSGAAWEAILKARNLAAYIPADLPNPQLELIILLPQAV
                     "
     gene            2509..4035
                     /locus_tag="VFA54_17755"
     CDS             2509..4035
                     /locus_tag="VFA54_17755"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZS52718.1"
                     /translation="MSTGTQSSPLLIGPADQFSALRQALDRLGYTESAICQRVGIDSI
                     FEFRTIREDRKTGVELNDALDTLIRLLMDEEVLSDVELRLLPPGTLEQMEQLGVVERI
                     GSQPVKWYATVVLYPVAGLYVASDRTFLPGGTEPRDLPTDAVYAAITGNTGRFVSFLP
                     QDPCEDLLDLCAGTGVGALIAGARFAKRAWAADLGQRCAHFAEFNRLLNGLDNVTSVQ
                     GDLYEAVGDRAFDRIVAHPPYVPNQDRSLLFRDGGEDGEQILARIIQGLPKHLRPGGS
                     FYCITAATDREGETFEQRIRRWLGESESEFDVLLVAIEYQRKPEAFLEAVVSAKGRFG
                     ELGPTSQLFRKLKVTGSFYGAVVLQRAESQRAVATARGVKGRTAGPEILEWFRKWETA
                     AAGAGFRAFLYNARPRMSKHLKLVVTHSLQEGGLLPSEFLLRSGYPFQSEAKIEPWVA
                     ALVGSCNGEFSTREIYQELKRQEVISPQLGEDGLAGVLCLLISAGFLDVPEFALPERS
                     "
     gene            4180..>4464
                     /gene="tssB"
                     /locus_tag="VFA54_17760"
     CDS             4180..>4464
                     /gene="tssB"
                     /locus_tag="VFA54_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HZS52719.1"
                     /translation="MPESLQHKLDRVRSPRVHITYDVETGGAIEKKELPFVMGVFGDY
                     TGMPEEPLARVKDRKFVEVSLDNFDNVMAAMKPHLAFSVENKLSDDPNAGQ"
BASE COUNT          896 a         1267 c         1437 g          864 t
ORIGIN      
        1 ggcgccgccg cacctccact tactggaatg gctggccgcc gaagaacggc ccgcccagcc
       61 tgagcccggg gagtttaccc ggctgtttca atcgccgccg cgcgccccgg tgaacgaacc
      121 ggcgccgccg aaatcggccg agccggcgcc gcccaaatcg gccgagccgc tatcgcccaa
      181 atcgggcgag ccgccggggg aattcactcg aatgtttcaa acgagtgcgt cgaagccgcc
      241 ggagcggccg acagagccgc cgccttcccg ggaacccggc gagtttacgc ggctgtttca
      301 atctccggcg ccggcgccca aggaggccga gcggagggaa tctgctccgg caccacggga
      361 agccgagcgg agggaatcgg ctccaccgcc caaggagccc gaacgacggg aatcaagtcc
      421 agcatcagcg cctggcgaat ttacgcgttt ttttcgcgga tctcaacccg agaccgcacc
      481 ggcgcgatca tttccaccac cgccaccgct ggctgccgct cccggcgccc cggcacctcc
      541 tccacaggcc gctcctgccg acaagggcgg cgagtttacg cgcatattcg gcagaccggg
      601 agcagaaccg cgagttccgc cacctgtcga agttccgcga agcgctccgg tgccgcccgc
      661 cgcgcccgta tcgcagcccc aggggccgag cgagtacacg cagatgatcg gccggccgcg
      721 caatgttcag acgcctccgc cccagcaggt ttccgtgccg gtgccgagcg ggcccgccat
      781 gccacagatg tccgcgccct cgatcccaca cgtgtccgcg ccggttatgc cacaggtatc
      841 cgcgcccgcg agaccgcagc cgtccaccgt agccgcgcca caatcgaact gggttctgat
      901 ggcgatcgtc gggatcgttt gctttcttgc cggcggttta ctcgtcttcc tgctgatgaa
      961 gcgctaaccg cttcagacgg cttgaggtaa gaggattata agctcgagtt gcgggttggg
     1021 tagatctgcc gggatgtagg ccgccaggtt gcgcgccttg agaatcgcct cccaggcggc
     1081 gccggactga ttcaggctga aatagtgata gttgagcttc accgggatgg cgctgggcgg
     1141 cgcggcggca taagtcaggg tcacacccgg caaggcctgc cggaccaggt gttcgatgtg
     1201 gctggccgag caaaccttta ccaactgcgg cgttttggcg acaatttccg caacggatgc
     1261 gccggcgctg accgccagat acatcctggt gttgaccaga tacttctcgt tgtcgatcga
     1321 cgtagcataa attgccggtt gtaccggttt gagcggcaga gagacgaagt tgctcggcac
     1381 gaccgcttcc agcagggcgc gcagtttttc atcgagctcg gtaaagcagc cgcccaaatc
     1441 gtcgtggtta tacgcgggca gatcgcgggg atggatttcg gttgagaagg tggtgagcgc
     1501 tccggccagg gtggtcatcg ccgaatagag agcttcaggt gggccaccgc gtgtttcaaa
     1561 caggtgacgg aacgccggta gggcggtatt gatggtgtaa aggagccaaa agttagcgat
     1621 atccgcggcg gtgaaatcgg cgaggctctg gttgcgctgg cggcgggtcc cggcaatggc
     1681 accagagcgg gcgatcagaa tctcgatgag ccggcgcgct atggaaacca ggtactcgct
     1741 ggcgcggaaa tcgagtagcg gcggaacaaa tcggggatct agctgaaacg tcccagtctc
     1801 ggagcgccga actcgcgcga ctctcagaat cgagaaacct tcgtgcgctt cggtttccga
     1861 caggagccgc agattgcggc gcgcaagttg cactggtttt tcggactggc cggtattttc
     1921 atcgcggacc aaggcgactt cggcgcgaaa acgggctccg cttccagtcg cattggcgat
     1981 attgacgccc cgttcccgat agtgagggat cgccagaaaa agatcgacag tggtctgatc
     2041 ggcttcaaaa caatcagcca gttgtttagg agccggaacg ttatcggaat cagggatatc
     2101 gaaaagcaag ccgtccggga gaattcccga cgcgtttgaa atgccgagcg cgccggcgga
     2161 cagagcctcc tggctgatcc gcagggaccg gaaaccggtg ggccgaaatg aaaggttctc
     2221 caggtgaaac tggagcaggt tttcgaggaa gcgatcttgc gcctgcaagt gttgaggggt
     2281 caggaatgtc cccttagtcc agatgacagg ctgtaacggc ttcactcgca acacagtaac
     2341 agaagtgcga cgatttcgaa acctgctcct cgtgcaggct ggtaaatctt tgcttgctca
     2401 tccggcaagt cgcgggtatg ctcgtagatt acacatcatt ttctcgggcg gccttgttgc
     2461 tggcaggcga ggctgtcgcg aacctcaggt atcatgccgg caagccgaat gagcactgga
     2521 acgcaatcct caccgttact gattggcccg gcggatcagt tttctgccct gcgccaggct
     2581 ctggaccggt tgggttatac cgaatccgcc atttgccagc gagtgggcat cgattctatt
     2641 tttgagttcc gcaccatccg cgaagaccgg aagaccggcg tcgagttgaa cgacgcactc
     2701 gatactctga tccgtctcct gatggacgag gaggtgctga gcgatgtgga actgcggctg
     2761 cttccacccg gaacactgga acagatggag cagctcggag tcgttgagag gataggcagc
     2821 cagcccgtca aatggtatgc gacggtcgtg ctttaccccg ttgccggact ctatgtggct
     2881 tcggatcgta cttttctgcc gggagggacg gaaccgcggg atcttccgac cgatgccgtg
     2941 tacgcggcga ttacgggaaa taccggccgc ttcgtgtcgt ttttgcccca ggacccctgc
     3001 gaggatttgc tcgatctgtg cgcgggcacg ggagtgggcg ctctcatcgc cggagcccga
     3061 ttcgcaaagc gagcgtgggc ggcggacctc ggacagcggt gcgcgcactt cgcggaattt
     3121 aaccggctgc tgaatggact ggataacgtg acctccgtgc agggcgatct gtacgaggcc
     3181 gtcggggatc gcgcgtttga ccggatcgtc gcccatccgc cctacgtacc caatcaagac
     3241 cgctcgctgc tgtttcggga tggcggcgaa gatggcgagc agatcctggc gcgaatcatc
     3301 cagggcttgc caaagcatct tcgccccgga ggcagctttt attgcattac ggcggcgacg
     3361 gatcgcgaag gcgaaacgtt tgagcagcgg atccggcggt ggctgggaga atccgaatcg
     3421 gaattcgatg ttcttctagt agcgatcgaa taccaaagga agccggaggc ctttctggaa
     3481 gcggtggtca gcgcgaaggg ccgattcggg gaactcggac ccaccagcca gttattccgc
     3541 aagttgaagg tgacggggag cttttacggg gccgtggtcc tgcagcgggc cgagagccag
     3601 cgggccgtcg cgacagcacg aggggtgaaa gggcgcaccg ccggaccgga aattctggaa
     3661 tggtttcgga agtgggaaac ggcggcggct ggcgcgggat ttcgcgcatt tctctataac
     3721 gcgcgtcccc gaatgtccaa gcacctgaaa ctggtggtca cgcacagttt gcaagagggc
     3781 ggcctgctgc cctccgaatt cctgttacgg tcggggtacc catttcagtc agaggcgaag
     3841 atcgagccgt gggtcgcggc gctggtggga agctgtaacg gggagttctc gacacgggag
     3901 atttatcagg agttgaaacg gcaggaggtt atctcgccac agttaggaga ggacggtctg
     3961 gccggtgttt tatgtttgct gatttctgcc ggattcctgg atgttcccga gtttgcgttg
     4021 ccggaacgaa gctaaggtta gctgatggat ctaacctata gtaaaagatt gtgaaagtcg
     4081 ggtatcggcg ctatcatact acgcagttcc aacccagcaa gctggtctga aactgcagtc
     4141 ggaaggctcg aatccgacgt ttgccgttag gagtgcaata tgccagaaag tcttcagcat
     4201 aagctagatc gcgtgcgttc cccccgggtt cacattacgt atgacgtgga aaccggcggc
     4261 gccattgaaa agaaagagct gcctttcgtc atgggggtgt tcggcgatta taccgggatg
     4321 ccggaggagc ccttagcgcg cgttaaggat cgcaaattcg tcgaggtcag cctggataac
     4381 ttcgataacg tcatggctgc gatgaaaccg cacttggcat tttcggtgga aaacaagctc
     4441 agcgacgatc cgaatgccgg gcaa
//
LOCUS       DASREK010000327         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2459002, whole genome shotgun sequence.
ACCESSION   DASREK010000327 DASREK010000000
VERSION     DASREK010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2459002"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..989)
                     /locus_tag="VFA54_17765"
     CDS             complement(36..989)
                     /locus_tag="VFA54_17765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS52720.1"
                     /translation="MRAFPAVLCILLCAAFARPAEAPDYRSRGDNHFFNLEYDEAIED
                     YSRLIAQDPQNPQAYNLLAAAQLYKELYRLGLLESSALRGDNRFLHQERPRPDPKAKA
                     RFEETLAKGREAAESLLARDPRNELALYALGTNYALRGNYEFMVEKAWFAALRSGGKA
                     HDYCERLRKINPNFVDAYLVLGVYEYVVGSLPLPVKMLAAIGGMHGSKQRGEAYVTRV
                     SREGKYARNDARVLLVILYRREKRPLEAARMIESLIADFPRNYVLGLELASMYSDAGQ
                     RERAITVFKDLLKKADEKAPGYERLPREAVVHKIEALEKGL"
     gene            1342..2850
                     /locus_tag="VFA54_17770"
     CDS             1342..2850
                     /locus_tag="VFA54_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52721.1"
                     /translation="MFSLEGPIAAHAEAVAAAGERSRASRLLRSIFSYPAALAAGLVF
                     ITVLSLSSRFDNPDLWWQLKIGEGIWNTHAIPSSDSFSFTAAGHPWTAHEWLAEVSMY
                     GAYRLGGYRGLMVWASALASLTFLLVYLLCWLLTGNALVSFRNAMVAWCFGTVGLNIR
                     PLIVGHFFLAAEILILELARMGRSRWLWLLPPLFAVWVNCHGSYVFGMALLAVYLVCS
                     RLKANSLFTAEEWDPRSRRRLAWTLALSAAALCCNPVGFRLWLYPFDTMLRQTSSMNA
                     IQEWLPPNLREPRTLAMLGAIVGIFLLSMLRRTTLSLRELLLLLMGAGLAVRYLRMMF
                     LFGIVISPILCRFGFGRAEKRDRPLANALILFGCLAAVIWSFPTEAALEKQVERMSPV
                     RAARFIRQANLTGRMLNDYAFGGYLIWSLPERKVFIDGRGDVFDWTGVLNDYVRWVNL
                     REDPALLLEKYKIDFCILAQGSSIAHVMPYLPGWKQIYADDVAVIFSRQTMR"
     gene            complement(2875..>3665)
                     /locus_tag="VFA54_17775"
     CDS             complement(2875..>3665)
                     /locus_tag="VFA54_17775"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZS52722.1"
                     /translation="TQLMAQGLATQDPEAKVKIYTQILLLDPNNQVAYNARKDAEEAL
                     EKQNAAKNQQATQSSQKLEDTLERETVRRQSLQKVEQALLTGDIKTASAQMALAKQAA
                     PNDPEVQRLDKVVGNLIEQKRRTTYLFSGAGLLVLLGLGAIVFLNRGEKDPYIEILNG
                     LDKGRRYNLDKEVIRIGAVAQDGGEKNDIVVTDLERAVSRFHCDIVRQSGKFYLLDRK
                     SANGTYIDGQRIPPGKPVRLKTGARVELGKSCALRLGMAKRKKK"
BASE COUNT          602 a         1112 c         1100 g          851 t
ORIGIN      
        1 tacgctgtac tcgccgcgcg cgaagccact tttggctaga ggcccttttc caaagcctcg
       61 atcttgtgga cgaccgcttc gcgcggcagg cgttcgtatc caggcgcttt ttcgtctgct
      121 ttcttcagaa ggtctttaaa aaccgtgatg gcgcgctcgc gctggccggc gtcggaatac
      181 atggaagcca attccaggcc gagcacatag ttccggggaa aatcggcaat caggctttcg
      241 atcatgcgcg ccgcttcgag gggcctcttc tcgcgccggt acaagatcac caataacacg
      301 cgagcgtcgt tccgggcgta tttgccctcg cgcgataccc gggtcacata ggcctctccg
      361 cgctgcttgc tgccgtgcat gcctccgatg gccgccagca tcttcaccgg aaggggcaga
      421 ctgcccacga catactcgta gacgcccagg acaaggtaag catcgacgaa attcggattg
      481 atcttgcgca aacgctcgca gtaatcgtgc gctttcccgc cgctccgcaa cgccgcgaac
      541 cacgcctttt cgaccatgaa ctcatagttt ccccggaggg cgtagttcgt gcccagggcg
      601 tagagcgcga gctcgttgcg cgggtcgcgc gcaagcaggg actcggccgc ctcgcgcccc
      661 ttggccaggg tctcttcgaa gcgcgctttg gccttcggat ccggacgcgg ccgctcctgg
      721 tgcagaaagc ggttgtcgcc tcgcagagcg ctcgattcca gcaggcccag gcggtagagc
      781 tctttgtaga gctgcgctgc ggcgagcagg ttgtaagcct ggggattttg agggtcttgc
      841 gcaatcaggc ggctgtaatc ctcgatggct tcgtcgtatt ccagattgaa gaaatgatta
      901 tcgccgcggc ttctgtaatc gggagcttcc gccgggcgcg cgaaggccgc acacaacagg
      961 atgcacagca ctgccggaaa tgcccgcatg gcgttctctg tatctagctt acagttctca
     1021 aggctcaggc gtcagcaggc cgcgcccaaa gcttccggct tgtgcaacct gcccgggccg
     1081 ctcgaatccc cttgcggttt cgcagtcttc tctggatgac tccggacgcc gcgcttgtcc
     1141 gctctaaggt aaaagcgccg gcgacccgcg gcggctgccg tatggcggag gcgccattcc
     1201 ggcgatatct tagagtggga gcgaagactg cggaacggaa ctggcggttc gcgggctgct
     1261 tggcggccgg cagcttcccc ggattggatc ttccacgcgg agcgcatccc ggctaaaaca
     1321 gattccgctc agaagggcaa aatgttttcc ttggagggcc ccatcgccgc acacgcggaa
     1381 gcagtcgcgg ccgcggggga acgctctcgc gcttccaggc tgttgcggag catcttttcg
     1441 tatcccgccg cgcttgccgc gggccttgtg ttcataacgg tcctgagcct gtccagccgc
     1501 ttcgacaacc cagacctgtg gtggcagttg aagatcggcg aaggtatctg gaatacacac
     1561 gccatccctt cttcggattc tttttctttc accgcggcag ggcatccctg gaccgctcac
     1621 gagtggctgg ccgaagtcag catgtacggg gcctacaggc tcggcggata caggggattg
     1681 atggtctggg cgtcggcgct ggcctcgctc acttttttgc tggtgtatct gctctgctgg
     1741 ctgctcaccg gcaacgctct ggtctcgttt cgcaacgcga tggtggcctg gtgctttggc
     1801 actgtgggcc tcaacatccg gccgttgatc gtaggccatt tcttcctggc ggccgagata
     1861 ttaattctcg agctcgcgcg aatgggacga tcccggtggt tatggctcct gccgccgctt
     1921 tttgccgtct gggtgaattg ccatggctcc tacgtattcg gtatggcgct gctggccgtt
     1981 tacctggtct gctcgcggct gaaggcaaat tctctgttca ccgcggaaga gtgggacccg
     2041 cgttcgcggc gccggctggc gtggacgctc gcgctatcgg cggctgcgct ctgctgcaat
     2101 ccggtcggct ttcggctctg gctctaccct tttgacacga tgctgcgcca gacctccagc
     2161 atgaatgcta tccaggaatg gcttccgccc aatctgagag aaccgaggac cctggcgatg
     2221 ctgggcgcca tcgtaggcat cttcctgctg agcatgctgc gccggacgac tttgtctctt
     2281 cgggagttgc tcctgctcct gatgggggcg ggcctcgccg tgcgctactt gcggatgatg
     2341 tttttgttcg gcatcgtcat cagtccgatt ctctgccgct tcggcttcgg ccgggcggag
     2401 aaacgcgacc gacccttggc aaacgcgctc atcctattcg gctgcctggc cgcggtgatc
     2461 tggtcattcc cgaccgaagc cgcgctcgag aagcaggtgg aaaggatgag tcctgttcgg
     2521 gcggcgcgtt ttatccgtca ggccaatctc acgggccgca tgttgaatga ctatgcattc
     2581 ggcggctatt tgatctggag cctgccggag agaaaggtct tcatcgacgg ccgcggcgac
     2641 gttttcgatt ggaccggcgt gctcaacgac tatgtgcgct gggtgaacct ccgggaagac
     2701 ccggccttgc tgctggaaaa atacaaaatc gatttctgca tcctcgccca aggctcttcg
     2761 atagcgcacg taatgccgta tttgccggga tggaagcaga tctatgcgga cgatgtggcg
     2821 gtcattttca gccgccagac gatgcgatga cgctcccggc cgggagcagt cttcctactt
     2881 cttctttcgt tttgccatgc ccagccgcag cgcgcagctt ttgccgagct ccacgcgcgc
     2941 gcctgtcttc aatcgaacgg gcttgcccgg cgggatgcgc tggccgtcga tatacgtgcc
     3001 attagcgctc ttgcggtcca gcagatagaa cttgccgctt tgccggacga tatcgcagtg
     3061 aaagcgggaa acagctcgct ccagatccgt tacaacaata tcgttctttt cgccgccgtc
     3121 ctgtgcgacg gcgccgatac gaatcacttc tttgtcgaga ttgtagcgcc gtcccttatc
     3181 gagcccattc agaatttcga tgtacggatc tttttcgcct cggttgagaa acacgatggc
     3241 tcccaggccg agcagcacca gcaatccggc tccggaaaac aagtaggtag tgcggcgttt
     3301 ttgttcgata agatttccca ccaccttgtc caggcgctgg acttcggggt cattgggagc
     3361 ggcctgtttc gcgagcgcca tttgcgccga agccgtcttt atgtcgccgg tcagcagcgc
     3421 ctgttctact ttttggagcg actggcggcg cacggtttct ctttccaggg tgtcttccag
     3481 tttttgtgat gactgggtcg cctgctggtt tttcgccgcg ttctgcttct ccaaggcctc
     3541 ctcggcgtct ttccgcgcgt tgtaggccac ctggttattc ggatcgagca acaggatctg
     3601 cgtgtagatt ttcactttgg cttccggatc ctgcgtcgcc agcccctggg ccataagctg
     3661 cgtga
//
LOCUS       DASREK010000328         5942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_1147937, whole genome shotgun sequence.
ACCESSION   DASREK010000328 DASREK010000000
VERSION     DASREK010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147937"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFA54_17780"
     CDS             <1..303
                     /locus_tag="VFA54_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52723.1"
                     /translation="GITGAAVEMCGTEGRLWIDRSRFEYYSLEKNARPIVVKAEGDLT
                     VDHVNNFLECCRTRKLPNGDVLIGHRSAQASHLGNLAYLQKRRIKFDPVREEIQPL"
     gene            312..1319
                     /locus_tag="VFA54_17785"
     CDS             312..1319
                     /locus_tag="VFA54_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS52724.1"
                     /translation="MADAQLVIDLIEGFRRSKVAFTAASMGIFDRLEQSPAGARPLAE
                     EYGSDADALERLLDACVAMGLLERAGDEYRNLPVSSRYLRRDSPDTLIGYVLYSDRVL
                     YPMWGCLQDAVREGSNRWEQVFGSKAGIFDKLFSTEEHKRAFLAGMHGMGNLSSPSII
                     AAFSLSRFRHLCDLGGGTGHLAIAACRRYPGLRATVFDLPGVAPETRRYIENAGLADR
                     IAVQPGDFFADPLPEADLFALGRILHDWPEPKILSLLRKICERLPAGGGLLVCERILN
                     PLKDGPVGSNLQSLNMLIVTEGKERTVAEYEALLEAAGFVNFRACTTGRPLDAMLATK
                     P"
     gene            1342..2538
                     /locus_tag="VFA54_17790"
     CDS             1342..2538
                     /locus_tag="VFA54_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZS52725.1"
                     /translation="MHNHAHPHRRRPAQVEFPGDSPSRRDFFKTLMHGALAGASILEL
                     AYHRAAWARAMAPSAGSTQLFDIEKVADGVYCALARAQAEINSNAAIFVNSSDVLIVD
                     AHSKPSAAASLIAQLKKEVTTKPVRYVVNSHFHWDHTQGNHAYRALGGKIDFIASEPT
                     RQLLSDLARQRLQASLDEAPKQIEALRSRAEKSRSSAEKAFCEDQIRQLQAYQAEMKN
                     YTLELPTITFAKSHVIKDKDHELHLEFHGRAHTAGDVVVFCPQKRVISTGDMIHGFLP
                     FIADGFPKTWPQTMDSVNRLAFDHILPGHGPVHHDHQRLINMRNYIEELTEKVEAGKK
                     AGKSIAELQRTITVASLKSMQSNGYSKYVSDNQYKYFPNFGPAAPLQDGVNTNIVEVY
                     NNLEKV"
     gene            complement(2645..3793)
                     /locus_tag="VFA54_17795"
     CDS             complement(2645..3793)
                     /locus_tag="VFA54_17795"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZS52726.1"
                     /translation="MPALGVAGSILLIVIVLVDAFEAVILPRRVSQRWRPTRLLYRGT
                     WIPWSALARRMKPGKRREAFLSLFGPLSTLLLLALWALLLITSFAVLHWALATPLNIA
                     PGRARLLSYFYLSGETFSTLGLGDITPQSSLGRLLIVGEAGLGFGFLAIVIGYLPVLY
                     QTFSRREASISQLDARAGSPPSAAQILLRWAPSRDPSALERFLQDWERWSAELLESHL
                     SYPMLSYYRSQHDNQSWLAAVTAVLDTCALVIAGIREVSPYQAQLTFAMARHAVVDLA
                     LVFNTPPKAPDQDRLPAADLARLRQALQSAALEMRGGRAAEEKIRELRGMYEPFVHGL
                     AWHFLMNVPPILAEPGRVDNWQTSAWMRRVAGINKLNQPEAPQDEHFE"
     gene            complement(3817..>5942)
                     /locus_tag="VFA54_17800"
     CDS             complement(3817..>5942)
                     /locus_tag="VFA54_17800"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZS52727.1"
                     /translation="ELLDRAGDIRSVLTAAGGRFPRLAEIAGGIREFRPLLRDLEGKI
                     LPDGRIADYASPALRRIRRETAQQKDAIRNSLERFVQAHSQEGSLQGEYITIRNDRLV
                     VPIRAAEKRRVEGVIHAASSTGQTLFVEPLETIDLNNRLVRLAEEELQEIRRILREMT
                     ARLARYAASIAASAAAVAALDVLFARARFGLEFGATVPRFSSPDQPKLHLRNARHPLV
                     EDVLRRRGDSAVPASLTLDAEHRVLILSGPNTGGKTVALKTVGLLALMARAGLPAPAE
                     EAEFPWFDQVLADIGDAQSIQESLSSFSAHIAAIRTMIETATPASLVLIDEMGAATDP
                     QEGGALGIAVAEHFRRTGAFTLISTHLPALKFYGVNTPGVLSASLGFDENTLQPNYRL
                     AVGLPGKSAGIEIARRLGIPAGIIERAEQALTTQDREIAALLGELHRRVEELRTAEEN
                     LERQREQLARREREIQREWEKREKARLEELERRVDAMLEKFNAQASQVVARISETSQG
                     RKAASEAARRAARAGRELREEFQATVLESLDAARRGPVLRPAAAIEPGTMVRLRGLGS
                     PGRVRRLLGYGRLEVEIGLIRMQAEVDDVLEVLPGAPPSTTAGKSLPGGVSVQASVPA
                     GSSLTEINVIGLTAEEARERVDKFLDTAVLAGAPAVRVVHGHGMCVLRKTLWEMFASH
                     PHVESYHPAEQHEGGAGATIVEIKP"
BASE COUNT         1140 a         1882 c         1817 g         1103 t
ORIGIN      
        1 ggaattaccg gggcggcggt ggagatgtgc ggtaccgaag gccgcttgtg gatcgatcgc
       61 agccgattcg agtactattc gctagagaaa aacgcccgcc ctatcgtggt caaagccgaa
      121 ggagatctca ccgtcgatca cgtcaacaac tttctggagt gctgccgcac gcgcaagctt
      181 ccgaacggcg acgtcctgat cggccaccgc tccgcccagg cctctcacct cggcaatctg
      241 gcctatctcc agaagcgccg catcaagttt gacccggttc gcgaggagat ccagccgctt
      301 tagcccagtt tatggccgac gcgcagctcg taatcgacct gattgaagga ttccgccggt
      361 cgaaggtggc gttcaccgcc gcgtcgatgg gcatcttcga ccgtttggag cagtccccgg
      421 cgggagcgcg tccgctcgcc gaagagtatg gctccgacgc cgacgccctc gaacgcctgc
      481 tggatgcatg cgtggctatg ggcttgctcg agcgcgcggg cgatgagtat cgaaaccttc
      541 cggtctccag ccgctatttg cggcgggatt cgcccgatac gctgatcggt tacgtgcttt
      601 actccgatcg cgtgctctac ccgatgtggg gctgccttca ggacgccgtg cgggagggga
      661 gcaaccgctg ggagcaggtt tttgggagca aggccggcat tttcgacaag ctgttttcca
      721 ccgaggagca caagcgggcc ttcctggcgg gcatgcacgg aatgggaaat ctcagctcgc
      781 cgtccatcat tgccgcattc agcttatcgc gcttcaggca tctttgcgat ctgggcggag
      841 gtaccggcca tctggcgatc gccgcctgcc ggcggtatcc gggtttgcgg gcaaccgttt
      901 tcgacctgcc gggcgtcgcc cctgagacgc ggcggtacat agagaatgcg gggctggcgg
      961 atcggattgc ggttcagccg ggcgattttt tcgccgaccc gcttcccgag gccgatttgt
     1021 tcgccctggg gcgcatcctc catgactggc cggagcccaa gatcctgtca ctgctccgga
     1081 aaatttgtga acgccttccc gccggcggag gcctgctggt gtgcgaaagg attctcaacc
     1141 ctctgaaaga cggcccggtc ggctcgaact tgcaatcgtt gaatatgctg attgtgacgg
     1201 aggggaaaga gcgtacggtt gctgaatatg aagcgctgtt ggaagccgcg ggcttcgtga
     1261 acttccgggc ctgcaccacg ggccgtccgc tcgacgccat gctggctaca aaaccttgac
     1321 tgaatcggga ggatttttcc aatgcataac catgcgcatc cgcacaggcg gcgcccggct
     1381 caagtggagt ttccgggaga ttcgccctcg cggcgggact ttttcaaaac tttgatgcac
     1441 ggcgccctgg cgggcgcttc cattttggaa ctggcgtatc accgcgccgc ctgggctcgc
     1501 gcgatggcgc ccagcgccgg cagcacgcag ctcttcgaca tcgaaaaagt ggccgacggg
     1561 gtctactgcg cgctggctcg ggcccaggcc gaaatcaatt cgaacgctgc cattttcgta
     1621 aactcctccg acgtcctgat cgtggacgcg cattcgaagc cctcggccgc tgcgtccctc
     1681 atcgcgcagc tcaaaaaaga ggtgacgacc aagccagtgc gttacgttgt caactcccac
     1741 ttccactggg atcacaccca gggcaaccac gcctaccgcg ccctcggtgg caagatcgac
     1801 tttatcgcga gcgagccgac caggcagctc ctgtccgatc tggcgaggca gcggctgcaa
     1861 gcctcgctgg acgaggcgcc gaagcagatc gaagcgctcc gttcgagagc ggaaaaatcg
     1921 cggtcttccg ccgaaaaggc cttctgtgag gaccagattc gccagttgca ggcttatcaa
     1981 gccgagatga agaattacac gctcgaattg cctaccatca ccttcgctaa atcgcacgtc
     2041 atcaaggaca aggatcacga gctgcatctc gaatttcacg ggcgcgccca cacggccggc
     2101 gatgtggtgg tcttctgccc tcaaaagcgc gtcatctcga ccggcgatat gattcacggc
     2161 tttctgccgt ttatcgcaga cggctttcca aagacctggc cgcaaaccat ggattcggtg
     2221 aaccggctgg cgtttgatca tatccttccg ggtcacggcc ccgtgcatca cgatcatcag
     2281 cggctcatca acatgcgaaa ttacatcgaa gaacttaccg agaaggtcga agcgggcaag
     2341 aaagcaggga agtcaatagc cgagctacag cgaactatta cggttgcctc gctcaaatcg
     2401 atgcagtcga acggctacag caagtacgta agcgacaacc agtataaata ctttccgaat
     2461 ttcggacccg ccgctccctt gcaggacggc gtcaatacca acatcgtgga agtctataac
     2521 aacctggaaa aggtctgacg atcaacggag gtatccggcc aggcagagca gcgcgctttc
     2581 tgcccgctgt cctgcaaacg ggccctgcgg cggcgggcgg cgcggcgctt ggatcttccc
     2641 gctttcactc aaagtgctcg tcctgcggcg cctccggctg attcagcttg ttgatgcccg
     2701 ccacgcggcg catccaggcg ctggtctgcc agttatccac gcggcccggc tcggccagga
     2761 tgggcggcac attcatcagg aaatgccacg ccaagccgtg gacgaagggc tcgtacatgc
     2821 ctcgcagctc tctgattttt tcttcggcgg ctctgcctcc gcgcatttcg agtgcggcgc
     2881 tttggagagc ctgccgcaaa cgcgcaaggt ccgcagccgg caagcggtct tggtccggag
     2941 cttttggggg agtgttgaac acgagagcca ggtccaccac cgcgtggcgg gccatagcga
     3001 aggtaagctg cgcctgatag gggctgactt ccctgattcc ggcgatcacc agagcgcagg
     3061 tgtctaatac tgcggtcacg gccgccagcc acgattggtt atcgtgctgc gagcgatagt
     3121 agctgagcat cggatacgag agatgactct ccagcaattc ggccgaccag cgctcccagt
     3181 cttgcagaaa gcgttccagc gcggacggat cccgggaagg cgcccagcgc agcaggatct
     3241 gagccgcgct gggcggcgag ccggctcgcg catcaagctg cgagatgctg gcctcccgcc
     3301 gggaaaatgt ctggtaaagc accggcaggt aaccgatcac gatagccaga aacccgaaac
     3361 ccaacccggc ttcccccacg atcagcagtc ggcccaggga cgattggggc gtgatgtctc
     3421 ccagaccgag ggtggagaag gtttcgccgc tcaagtagaa atagctcagc aggcgggcgc
     3481 gtcctggggc gatgttgaga ggggttgcca gggcccaatg caaaaccgcg aagctggtaa
     3541 tcaggagcag ggcccagagc gcaagcaata ggagagtcga gagcgggccg aacaaactca
     3601 agaacgcttc gcgccgcttg cctggcttca tccgccgcgc caaggccgac caggggatcc
     3661 atgtcccgcg atacaggagg cgggtgggcc gccatcgctg gctgacgcgc cgcggcaaga
     3721 tcacggcttc aaacgcatca accagcacga tcacgattaa caggatgctc cccgctacgc
     3781 cgagcgcagg cattcgcccc ccccaaaatt ctcagctcaa ggcttgatct cgactatggt
     3841 agccccggct ccgccttcgt gctgctcggc cggatggtag ctttcaacat gtgggtggga
     3901 agcgaacatc tcccagagtg ttttccgcag aacgcacatg ccgtggccat gcacgacgcg
     3961 aacggccggc gctcccgcca gtacagcggt atcaagaaac ttgtccacgc gttcgcgggc
     4021 ctcctcggcg gttaagccga tcacgttgat ttcggtcaag gacgatcccg ccggcacgga
     4081 cgcttgaacc gagacgccgc cgggcagaga cttgccggct gttgtggagg gaggcgctcc
     4141 ggggagcact tcgagcacat cgtcaacctc cgcttgcatt cgaataagtc cgatctcgac
     4201 ttccagacgg ccgtacccca aaagccgccg cacgcgcccg gggctgccca ggccccggag
     4261 ccgcaccatc gttcccggct ctatcgccgc ggccggacgc aaaaccggcc cgcgtcgcgc
     4321 cgcatcgagc gactcaagca cggtggcttg aaattcctct cgaagttcgc gcccggcgcg
     4381 cgcggctcgg cgcgctgcct cggacgccgc cttgcggccc tgagaagtct cgctgatcct
     4441 ggcgacaacc tggctggcct gggcgttgaa tttttcgagc atcgcatcga cgcgtctctc
     4501 gagctcttcg agcctggctt tctcgcgctt ctcccattcc cgctggattt ctctttcgcg
     4561 gcgcgcgagc tgctcgcgct gacgctccag attctcctcg gcggtccgaa gctcctcaac
     4621 ccgccggtgc agctcgccga gcagagctgc gatttcgcga tcctgagtgg tgagagcctg
     4681 ctcggcgcgt tcgatgatcc cggccggaat gcccagccgc cgtgcgatct cgattcccgc
     4741 tgattttccg ggtaatccaa ccgccagccg gtagttgggc tgcaaagtgt tttcatcgaa
     4801 gcccagcgaa gcgctcagca ccccgggcgt attcaccccg tagaacttca gggcgggcag
     4861 atgcgtagaa atcagcgtaa aggctcccgt gcggcgaaaa tgctccgcca cggcgatccc
     4921 cagcgcgccg ccctcctgtg gatcggtggc cgctcccatc tcgtcgatga gcaccagtga
     4981 cgccggcgtc gcggtttcga tcatggtgcg aatggccgct atatgggcgg agaagctgct
     5041 caagctttcc tgaatggact gggcgtcgcc gatgtcggcg agcacttgat cgaaccaggg
     5101 aaactcagcc tcctcagccg gcgccggcag cccggcgcgc gccatcaggg ccagcagtcc
     5161 caccgtttta agagcgacag tcttaccgcc cgtattcggg ccgcttagga tcaacacgcg
     5221 atgctccgca tcgagggtca gagacgcagg cacagcggag tcgccgcggc gcctcagcac
     5281 gtcttccacg agcggatgtc tggcgttccg cagatggagc ttgggctggt ctggggaaga
     5341 gaagcgcgga acggtcgcgc caaattccaa accgaagcgc gcgcgcgcaa acaggacatc
     5401 gagagccgcc acggcagcgg ccgaagcggc gatggacgcg gcgtaacgag caagccgggc
     5461 ggtcatctcg cgaagaatgc gccggatttc ttgcaattct tcctcagcga gacgtaccag
     5521 ccggttgttg agatcaattg tctcgagcgg ctctacaaag agcgtctgac cggtagaact
     5581 ggcggcatga attacgcctt ccacccggcg tttttcggcc gctcttatcg gaactaccag
     5641 ccggtcatta cgaatggtta tgtactcgcc ctgcaagctc ccttcctggc tgtgcgcctg
     5701 cacgaagcgc tcgagcgagt tgcggatagc gtccttctgc tgcgctgttt cccggcgaat
     5761 gcggcgcagc gcgggactcg catagtcggc aatccggcca tcgggaagaa ttttgccttc
     5821 cagatcccgc agcaagggac ggaattccct gatgccgccg gcgatttccg cgagccgcgg
     5881 aaaacggccg ccagcggcgg tgagcacgga ccggatgtcg ccggcgcggt ccagcagttc
     5941 ca
//
LOCUS       DASREK010000329        16136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_163952, whole genome shotgun sequence.
ACCESSION   DASREK010000329 DASREK010000000
VERSION     DASREK010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 16136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16136
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163952"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFA54_17805"
     CDS             complement(<1..314)
                     /locus_tag="VFA54_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52728.1"
                     /translation="MYSLLFLAGLSFLICFFLTPLVRNFLNRTGMLDRPDRNRKFHER
                     PIPRLGGVPIVAAMALSLAIFSQVPFSGSDVFRSQWHTLLHLLPGAALVFLIGVVDDL
                     AGL"
     gene            complement(336..2108)
                     /locus_tag="VFA54_17810"
     CDS             complement(336..2108)
                     /locus_tag="VFA54_17810"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HZS52729.1"
                     /translation="MKRALERLAAYWYSLRYQQATQISGRLQYWLAWKIYPRFPSAVA
                     TYCELAARACQTNPDSFSSRPAIAGSSDGGSNRFSLLNETVDFNNPVDWNPPGRSLLW
                     LFHLHYFDWAQACDAETLSRQIESWIDSNPPGSWPGWHPYPTSLRIVNWLRALQGALH
                     LQNEKEPGSSTSASRFKARIEQSLAQQAAFLEINLEHHLGGNHLLENARALLMAGLHF
                     EGAPARRWRAAGLALLRAEISQQVLPDGGHCERSPYYHARVTDLLEDVIRTLADRGCP
                     APSDLTEAAARMASFRGALRHRDGSLPLFHDSLGPAPPRNSAPSCARISFPFTGYYIL
                     ESSQGRMIADYGAPGSSPNPAHQHAGIFSYEISSARGRVVVDSGTATYEAGPGRDRLR
                     ATPAHNTVCVDGLDQFQVWRAFRAGRRAWVGSVVEIRRPDCEILSASHNGYRRLGVEH
                     RRSIVHLAGAGWLVVDDLLGRKRRHLESFVHFAPEMAPEIHGSRVCLPPLGWALVVFG
                     FEAPPAVRADFFSPAVGVVQPSKTLVLKAEASLPLRFGYWLGYDASVTWDGSCRVQIE
                     AADRSVVVQLGPIGSNAAFEVRYN"
     gene            complement(2092..3351)
                     /locus_tag="VFA54_17815"
     CDS             complement(2092..3351)
                     /locus_tag="VFA54_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS52730.1"
                     /translation="MRILYVSQYFPPEMGAPAARVSQLASRWRDMGHDARVLTGFPNH
                     PGGRIYPGFQRQFRRGFDSEQYQGVTVYRTWLYPAANRGLVRRSANYASFCISAALRG
                     CLLNFQPEVVIGSSPQLLCAQAASWIARRYGARFIFEVRDLWPESLAAVRACSADSRL
                     YRALDRLAGALYDRSWKTVVVTEESKRRLCERGLDASRLSVVPNGVDAAMFRPDVPPA
                     RHPELAGKFVVSYIGTLGMAHSVSTILHAADLLRGRADIHFLLVGNGAEREALLQQRQ
                     ERGLDNVTFVEQLPWSAVPSYLSLSSACIVHLARSPLFETVLPSKMFEIMASAKPVIL
                     GVRGEAERLLEAAQAGLACEPESPAGLRDAILQLAANPGLCRALGQNGREYVLKHCSF
                     ERRAAEYIAALEPGRPEPGVQTHEAGA"
     gene            complement(3351..5474)
                     /locus_tag="VFA54_17820"
     CDS             complement(3351..5474)
                     /locus_tag="VFA54_17820"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HZS52731.1"
                     /translation="MRQLVQNRKSGAVELLEVPSPVCGPRAVLVQTLASAVSAGTERH
                     MLKLGRQSVLHTAWERPDLVARVASKFEREGLAATIRAIREKTENTVALGYSSCGRVV
                     EAGAGIEDLRPGDLVACAGQDYASHAELVAMPRTLVARVPEAVSVEHAAFATLGAIAL
                     EGVRLARVQLGETAAVIGLGLLGQITVQLLLAAGCRVVAVDLDPARVELAQRAGATVA
                     VANGDLPSLNPGSVAGTPHGADAVLITADSASSAPVELAAQLARERGRVVAVGLIKTD
                     LPRHEFFRKELQFVVSRSSGPGRYDPSFEVEGRDYPYAYVRWTQTRNLEAFLDLLARG
                     RVSIDHLITHRYPFHEAPAAYELLLGGAPQMGIVFHYPDASPSLSHRLSLSARTPRPG
                     RPSVGCIGAGSFARSVLLPAIAEHKDVQLAAIASVNGIRAREAAQRFGFRAALTSAGE
                     LLAQPDVDTVFIATPHASHGPLICRALESGKHVFVEKPLCIEPVELEQIAEAHGRAGR
                     LLMAGFNRRFSPFAAEARRLLASRRGRLSFNYRINAGPLPPDHWLSRAEEGGRIVGEV
                     CHFIDLIAFLAGSLPVQVYAAGSPQGGAQLNLTLEDGSTGVIDYITANQARLSKERLD
                     IFGPDLVIEMDDFRSALVHQRRNRSRWKWRRQDKGHRAEIGAFLKAVREGGPAPIDFE
                     SLARTTRATFAAMRSLQTGAPVSCV"
     gene            complement(5630..5788)
                     /locus_tag="VFA54_17825"
     CDS             complement(5630..5788)
                     /locus_tag="VFA54_17825"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HZS52732.1"
                     /translation="MSGPSLLHEFWLFLRQNKKLWLLPIVIVLLLLGALLLFAQSSAL
                     APFIYTIF"
     gene            complement(5791..6192)
                     /locus_tag="VFA54_17830"
     CDS             complement(5791..6192)
                     /locus_tag="VFA54_17830"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HZS52733.1"
                     /translation="MINPAPGRAQARKTTLSVGAVLTFAGALSLLRHHWLRAEIAGGA
                     GILLLLAALLAPRQALRFHRAWMKFAAALGYINSRIILSVMYYGVLTPLGFILRMAGR
                     DPLHRRAAAQPSYWIPRPRTRQDRNQFERLF"
     gene            complement(6185..8014)
                     /locus_tag="VFA54_17835"
     CDS             complement(6185..8014)
                     /locus_tag="VFA54_17835"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HZS52734.1"
                     /translation="MWILGLSAYYHDSAACLVRDGAVVAAAQEERFSRKKHDHRFPAH
                     AVAFCLRQGGISASELEAVVFYDKPLQKFERLLETYLDYAPRGLRSFLQAMPLWLREK
                     LWMREEIARQCGFKGKVLFTEHHESHAASAFFPSPFESAAILTIDGVGEWATSSYGRG
                     EGNQIDLLAETRFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGQPRYTQKILDEL
                     VDLREDGSLRLNMRYFNYCQGLTMTSEAFHELFGGPPRRPESPLTAREMDLARSVQDV
                     TEEAMLRMARHVHRQTGERNLCLAGGVALNCVGNGRILREGPFESLWIQPAAGDAGGA
                     LGAALSVWYQYYNQPRSAGAVCRGRTDGMSGSYLGPEYSADEILEFLESSGARYTRAT
                     GAELCARVAALLAEEKVIGWFQGRMEFGPRALGARSILGDPRSPRMQSVMNLKIKFRE
                     SFRPFAPSVLRERVADYFEMDCDSPYMLLVAPVREHRRLPMTQEQQQLFGIEKLNVPR
                     STIPAVTHVDYSARIHTVRREENPLYYDLLCAFERLTGCPVLVNTSFNVRGEPIVCTP
                     EQAYTCFMRTEMDYLVLGDFILDKREQGEFVEARTWQEEFQLD"
     gene            8222..10165
                     /gene="asnB"
                     /locus_tag="VFA54_17840"
     CDS             8222..10165
                     /gene="asnB"
                     /locus_tag="VFA54_17840"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZS52735.1"
                     /translation="MCGIAGIFGQSDEHLARQFCRVLEHRGPDDEGFLTSASGPITLA
                     SRRLAILDLSPAGHQPMQTAGGRCTLVYNGEVYNFAEIRSELQSRGRQFRSRGDTEVV
                     LEALAEKGTAALGEFNGMFALAFWNARERKGLLARDPFGVKPLYYAPLPGGRLIFASE
                     IKALLLHPELAAGLNLCALPQYLAFLWTPDPATLFEGIYKLPPGHLLEWQEGAFSIRR
                     YYELPALACREEVSEEGAVEGLRLHLRRAVQRQRVSDVPVGLFLSGGLDSSALLAEMA
                     GASPLGPAARQEIRCYTAGFPAEDNAFDQFGSDPPYARAVARRFGAHLREFEIRAGMT
                     DLLPKLLYHLDEPLADPAVINTYLICKQAREDGARVLLSGQGADELLGGYRRHIAPLL
                     NERLQGAPAALLGAVARLSKRLPAGLAGRAGGALRRARKLLEPVNRPPSEQFVHYCQW
                     LHDDDVRSLLAPEVLATLGLWRHPSAFTEEVLGQSPSGGLLDRMLYRDLKTFLPALNL
                     TYTDKMSMAASVEARVPYLDLELAEFCWRLPQRYKVRGATGKWLLRRAMRGTLPAEVL
                     WRSKTGFGAPVRQWMRHELKEMAADLLSPAALRAGGVFCAEGVEALRASFADGQGDHG
                     YTLWALLVFQLWREVFLRQPAAR"
     gene            complement(10162..10950)
                     /locus_tag="VFA54_17845"
     CDS             complement(10162..10950)
                     /locus_tag="VFA54_17845"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS52736.1"
                     /translation="MIVQWSSGNASEGMSEDILTDLAGPISRHPWYQARARMAVALLK
                     KVNVLPPARVLEAGCGWGLNLEALERAGYRAVGLDIARRSLEKLDRPGRELIEADLTR
                     PLPPHAEQYDAVLALDVIEHLDDDRGTVARLAQLTKPGGFTIVGVPALPELFSDFDVV
                     QGHRRRYLPESLRQAFSGTGLVIEHMLWWGSWMVPLLKRQRGRRPRAARAEAPAAAYK
                     RYLALPPWPAPLLMRAAFGLSQTRSLRGESKTGTSLFALARRVG"
     gene            complement(11007..11957)
                     /locus_tag="VFA54_17850"
     CDS             complement(11007..11957)
                     /locus_tag="VFA54_17850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS52737.1"
                     /translation="MIPCFNEEAVLPMLEKRLLEALGGAGISWELVFVDDGSSDRTLE
                     ILSEMHRRDGRFKVISFSRNFGHQTAISAGLAYASGEAVAIIDADLQDPPDLLATCLR
                     CWKDGYQVVYAVRQKRKEGPLKRAAYSIFYRLLRVIADIHIPLDTGDFCLMDRSVVNV
                     LRSMPERNIFVRGMRAWAGFRQIGIPYERSARAAGETKYPFRKLLRLALNGIFSFSTF
                     PLRLATWFGLSIVGLCALVVLLVSIWRFSGLEFMGHVAADIPGWAAGIVSVFFLGGVQ
                     LLILGLIGEYLARIYDEVKLRPRWVVSRSLGFSRQPSQVE"
     gene            complement(11986..12762)
                     /locus_tag="VFA54_17855"
     CDS             complement(11986..12762)
                     /locus_tag="VFA54_17855"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS52738.1"
                     /translation="MWHDKKVAVVFPTYNEKDSIRSAILDFWAGGLVDEILVVNNNAA
                     PGTSEEIAGTGAREVFESRQGYGYALLRGIDECSAELIILAEPDGTFSGHDVIKLLAY
                     SDDMPVVFGTRTTREFIWEGANMGWFLKSGNVAVAKLTEFLFNTTILTDMGCTLRLFR
                     REALDLIRPHLAIGGSHFGSQLLMEVIAHQIPFVEIPVNYRRRVGRSSVTGDFWKAFS
                     LGVRMIRLVLAYRFGFYRRARISWESWAAQKPAESVSTHA"
     gene            complement(12749..14341)
                     /locus_tag="VFA54_17860"
     CDS             complement(12749..14341)
                     /locus_tag="VFA54_17860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS52739.1"
                     /translation="MKQAAAESAAVKIDSGTGRLWPLAAIGILLLYNAATLGRVPAFP
                     NDDDGCYASAAYQFWNTGRPGVPGYQDAAGLGADVWAFGRTAAAFQGLFLHFGGVSLL
                     HALLPSFLAGLGVLTVTAALAAVLWDRQTAVLAPLLLAASGKFFDACRWARPDMLLAL
                     FFLLSLWLAASARPGKPYVKLAAAGLAMGLSGDVHLNGFLIAFIPFLFWLFLRREPAR
                     VRWLGGAIFSAGVAAGVLFWLRLHYWPNPQAMLHQAAVYGGKTHGLRIADLGLAAAFK
                     MEIQRYTDWFWDARGHRHALEGLCILAAGVWLWLREGRTGRALVAAWSAFFAVALLFM
                     SNRFGWYLILPWPLFAIWMARLFQAFPARRIARGALALLLAACALNLGLWHWKARQDV
                     PFSSRLAELRRVIPGDDPVLGNDVFWFAFWDKDFTSSEYLYLRRLEAENGRGPDASGW
                     IAAQQRRKWRYIVASGELRLFLDPDFPLPLAVASRAGREDEIRQARAFSLERCSVVRR
                     FAGVADTILVLRVNENNPDVAR"
     gene            complement(14338..15870)
                     /locus_tag="VFA54_17865"
     CDS             complement(14338..15870)
                     /locus_tag="VFA54_17865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS52740.1"
                     /translation="MSFFRDSLEAGPGAAGAARPNWTRRRLALLFWAFAGAILAFDLI
                     LLEQIPYAATVDDGAYAAAAFSFVHTGRAGLPMDKGLFDLDRDLIMYGRIPAALVGLC
                     ELALGPNCFASRLPGLLAVWFSVPLVLLIARECRVPELLAATGSLLWLLSVPAQMLAH
                     TARPDPLLMLFTVVCCLLLLRALRNDDWRLFLLAGFIAGLSLEVHMIGAALIAALAAA
                     VLTSEPAGRIRNSALVCLGAAPAAFLWLYDHAWSQPDLWRIQWVGFWQAAGPSPLLLG
                     PKAIITSAVARYRSYFWDARYHRYALEGFFLAAGVCAGLMRRNPVRRLAAIVVLFHVF
                     LAILSALPHAGYMAAVYPLGSILIASAFFGSSRRVVFLAGLSLIAFYCAQMGYWAAHR
                     DVPLPGRVRADVLRHAGDKPFLAADNLWFYFPRDRNLRSVVSANFVQRWAPDLPWEQA
                     FRRYLNRQQVQFVVVDKVFREFLLPSQWAFLARNMTPLAEYNYGKAGSIQIYSLHLPG
                     KP"
BASE COUNT         3427 a         5019 c         5126 g         2564 t
ORIGIN      
        1 agaccggcca gatcatccac cacgccgatc agaaaaacca gagccgcccc ggggagcaga
       61 tgcagcaaag tgtgccactg ggagcgaaac acatctgagc ccgagaacgg aacctgcgaa
      121 aagatggcca gcgacaaggc cattgcggcc acaatcggaa ctccgcccag ccgcggtatg
      181 gggcgctcgt gaaatttccg gttccgatcg gggcggtcga gcattccggt gcggttcaga
      241 aaattgcgca ccaggggcgt caggaagaag cagatcagga aagaaagacc tgccaaaaag
      301 aggaggctat acattatccc ggcccgcccg agcggttaat tgtaacgcac ctcgaaggca
      361 gcgttgctgc cgatcggccc cagttgtaca acaacgcttc gatccgctgc ttcgatctgc
      421 acccggcagg atccatccca agtcacagaa gcatcgtacc cgagccaata cccgaaacgg
      481 agaggcaggc tggcttcggc tttcaacacc agcgtcttgg acggttgcac aactcccaca
      541 gcgggactga agaagtctgc ccgcacggcc ggcggcgcct cgaagccgaa gaccacaagc
      601 gcccagccga gcggcggcag acacactcgg gagccatgaa tttccggcgc catttcggga
      661 gcgaaatgca caaagctctc caggtggcgg cgctttcggc caagcagatc atccaccacg
      721 agccagcccg cgccggcgag gtgcacgata gacctgcggt gctccacgcc aagccgcctg
      781 tatccgttgt gcgaagcgga gaggatctcg caatccggtc tccggatctc gacgacagat
      841 ccgacccagg cgcgccggcc ggcccgaaat gcgcgccaga cctgaaactg gtccagcccg
      901 tctacgcaga cggtattgtg agcgggcgtg gcgcggagac gatcgcggcc gggccctgcc
      961 tcatacgttg ccgttccgga gtccacgacg acgcggccgc gggcgctcga aatctcgtaa
     1021 gaaaatatac cggcgtgctg gtgcgcgggg ttgggcgagg aaccaggggc gccatagtcc
     1081 gcgatcatcc ggccttgaga actctcgaga atgtaatacc ccgtgaaggg aaacgaaatt
     1141 ctggcgcaag aaggcgccga attgcggggc ggcgctggac cgagcgaatc gtgaaagagg
     1201 gggaggctgc cgtcccggtg gcgcagtgcg ccccggaagg acgccatgcg cgccgccgct
     1261 tcggtgagat cggagggggc ggggcagcct cgatcagcta gcgttcggat cacatcttcc
     1321 agcaggtccg tcactcgcgc gtgataatag ggagagcgct cacagtgacc gccgtcggga
     1381 agaacctgtt gggagatctc agcccgcagc agagcgaggc cggcggctcg ccagcgccgc
     1441 gccggagctc cttcgaaatg aagccccgcc atcagcaagg cgcgggcgtt ctcgagcagg
     1501 tgattcccgc ccagatggtg ttccagattg atttcgagaa aagccgcttg ctgtgccaga
     1561 gactgctcga tgcgggcttt gaagcgagag gccgaagtcg aactgcccgg ctctttctca
     1621 ttttggagat ggagggcgcc ctgaagagcc cgcagccagt ttacgattcg cagcgacgtg
     1681 ggataggggt gccagccggg ccaactgccc ggcgggttcg agtcaatcca gctttcgatc
     1741 tgccgcgaca gcgtttcagc gtcgcacgct tgcgcccagt cgaaatagtg caagtggaag
     1801 agccagagca gagagcggcc gggcgggttc caatcgacgg ggttgttgaa atcgacggtc
     1861 tcgttgagca acgaaaaacg attgctcccg ccgtcgctgc tgccggcgat tgccgggcga
     1921 gacgaaaagg aatcagggtt ggtctggcag gcgcgcgcgg cgagctcaca ataggtcgcg
     1981 acagccgagg gaaagcgggg atatattttc caggctagcc agtattgaag gcggccggag
     2041 atctgcgtcg cctgctggta ccgcaggctg taccagtaag cggcgagccg ttcaagcgcc
     2101 cgcttcatgg gtctgaacgc cgggttccgg acggccgggt tccagcgcgg caatgtattc
     2161 ggcagcgcgg cgctcaaagg agcaatgttt aagaacgtat tcacggccat tctgtcccag
     2221 cgcccggcac agaccaggat tggccgcaag ctgcaaaatg gcgtcgcgca gtccggcagg
     2281 cgattccggt tcgcacgcca ggcccgcctg ggcggcctca aggagacgct cggcctcgcc
     2341 ccggactccc aagatcaccg gcttcgccga ggccatgatt tcaaacatct ttgacggcag
     2401 aaccgtctcg aaaagcggtg agcgcgccag gtggacgatg caggcgctcg atagcgacaa
     2461 atacgaggga accgccgacc agggcaactg ctcgacgaaa gtcacgttat ccagcccgcg
     2521 ctcctgccgc tgctgcaaca aagcttctcg ctcagcgccg ttgcccacga gcaaaaagtg
     2581 aatgtccgcc cggccgcgca gcaagtcggc ggcatgcaga atggtggaaa ccgagtgcgc
     2641 catacccaga gttccgatgt aggaaacgac aaacttgccc gccagttcgg gatggcgggc
     2701 cggcggaacg tccggccgga acatggctgc atcgacgccg tttgggacga cggaaagccg
     2761 ggaggcatcg aggccgcgct cgcaaagccg gcgcttggat tcttcggtca ccacgaccgt
     2821 tttccaggag cgatcgtaca atgcgcccgc caggcggtcc agcgcccgat aaaggcggga
     2881 atccgccgag caggcccgga ccgcggccaa agactccggc catagatccc gcacttcgaa
     2941 aataaagcgc gcgccgtatc tccgggcgat ccagcttgcc gcctgcgcgc acagcaactg
     3001 cggggagctg ccgatgacca cctcgggctg aaaattgagc aggcacccgc gcagggccgc
     3061 cgaaatgcag aaactggcat aattggcgga gcgccgcacc aggccccgat tcgcggccgg
     3121 atacagccag gttcgataca ccgtgactcc ctgatattgc tcggaatcaa agccccggcg
     3181 aaattggcgt tggaatcccg gatagatccg gccgccggga tgattgggaa aaccggtgag
     3241 cacccgggcg tcatgtccca tgtcgcgcca gcggctcgca agctgcgaca cgcgcgctgc
     3301 cggcgctccc atttcgggcg gaaaatactg gctgacgtag agaatccgca ttagacgcaa
     3361 gagaccggcg caccggtttg gagcgaccgc atcgcggcaa acgtcgcccg ggtggtgcgc
     3421 gcgagactct caaaatcgat cggcgccggg ccgccttccc gcacggcctt taggaaagcg
     3481 ccgatttccg cgcggtgccc tttgtcctgg cggcgccatt tccagcggct cctgttgcgg
     3541 cgctgatgga ctagcgccga gcggaaatca tccatctcaa tgaccaagtc gggtccgaaa
     3601 atatccagcc gctccttgct gaggcgcgcc tggttggccg tgatgtaatc gataacgccg
     3661 gtcgagccgt cttccaacgt aagattcaat tgcgcgccgc cttggggcga gcccgccgcg
     3721 tacacctgaa ccggcagcga accggccaga aatgcgatga gatcaatgaa atggcacacc
     3781 tcgccgacaa tgcgtccgcc ttcttcggcc cgcgagagcc agtgatccgg cggcaaaggg
     3841 ccggcgttaa tgcggtagtt gaagctgagg cgacccctcc gcgaggccag taaccgccgg
     3901 gcttcggcgg cgaaaggcga gaagcggcga ttgaagccgg ccatcagcaa gcggcccgcg
     3961 cgcccgtgcg cttctgcgat ttgttccagc tcgaccggct cgatgcacag cggcttctcc
     4021 acgaagacgt gtttgcctga ttcgagagcc cggcatatca gcgggccgtg gctggcatgc
     4081 ggggtggcga tgaaaacggt gtccacatcc ggttgagcca gaagctcgcc ggccgaagtc
     4141 agcgcagctc gaaagccgaa tctctgggcc gcttcgcgcg cccggatgcc gttcaccgaa
     4201 gcgatggcgg caagctgcac gtctttgtgc tccgcgatgg ccggcagcaa gacactgcgg
     4261 gcgaaggacc ccgccccgat gcagccgaca gaaggccgac ccggacgcgg agtgcgggcg
     4321 gagagcgaca ggcgatggga gaggctcggc gaggcgtcag gataatgaaa aacaatgccc
     4381 atctgcggag cgccgcccag gagcagctcg taggcggcgg gcgcctcatg gaagggatag
     4441 cgatgcgtga tcagatgatc tattgaaacc cgcccccggg ccagcaggtc gaggaacgct
     4501 tccaggttgc gcgtctgggt ccagcgcaca taggcgtagg ggtagtcccg gccttccacc
     4561 tcgaagctgg gatcgtaacg gcccgggcct gacgaacggg agaccacgaa ttgcagttcc
     4621 ttgcgaaaga attcgtgccg cggcaagtcg gtttttatca agcccacggc gacgacgcgt
     4681 cctctttctc gggcgagctg cgccgccagt tccacagggg cgctggacgc agagtcggct
     4741 gtgatcagga cagcgtccgc gccgtggggc gtgcccgcga ctgagcctgg attcagggaa
     4801 gggagatcgc cgttggcaac ggcgacggtc gccccggcgc gctgcgctaa ctccacgcga
     4861 gccggatcga gatccactgc gacgacacgg catccggcgg ccagcaggag ctggactgtg
     4921 atctgcccca gcaatccgag gccgatgacg gccgccgtct ctcccaactg tacccgggcc
     4981 aggcgcacgc cttccagcgc gatagccccg agcgtcgcaa aagccgcgtg ctccacggag
     5041 accgcttccg gcacccgagc cacgagcgtc cgcggcatgg ccaccagttc ggcgtgtgaa
     5101 gcgtagtctt gtccggcgca agccaccaga tcgcccggac ggaggtcttc gatcccggcg
     5161 cctgcctcaa ctactcggcc acacgagctg tagccaaggg cgaccgtatt ctccgtcttc
     5221 tcacggatcg cgcgaatggt ggccgccagg ccctcgcgtt cgaatttgga cgccacgcgg
     5281 gcgacgagat cgggcctctc ccaagcggta tgcaggaccg actgccgccc cagctttaac
     5341 atatgccgct cggtgccggc gctcaccgcg gaggcgagcg tttgcacgag cacagcgcga
     5401 ggtccacata ccggcgaggg gacctcaagc aactcgaccg cacccgattt ccggttttgt
     5461 actagctgcc gcacgtcaat ctccgcaatg ccaagaaacc cagcccaacc ccgtctctct
     5521 tatgcagctt attggaagcc tgtgaacggc cgcaaggcag cttgcttgct acgccggctt
     5581 gagcggcttg acattgggtg gccgctgctc cgctgtggct ggcgcacaat cagaaaattg
     5641 tatagatgaa aggagcgagg gcggaacttt gggcgaaaag cagcagagcg cccagcagaa
     5701 gtaagacgat cacaatgggc aggagccaaa gcttcttgtt ctgccgtaga aagagccaaa
     5761 attcgtgaag caacgacggg ccgctcattt tcagaaaagt ctctcgaact gattgcgatc
     5821 ctgccgcgtc cgggggcgcg gaatccagta actcggctgc gcggcagcgc ggcggtgcag
     5881 cgggtcccgg cccgccatcc gcagaataaa gcccagcggg gtcaaaactc catagtacat
     5941 gacagagaga atgatgcggc tgttgatgta gcccagggcc gccgcgaact tcatccatgc
     6001 ccgatggaat cggagcgctt gccgcggagc gagcaacgcc gccagcagca gcagaatacc
     6061 cgcccctccg gcgatttcag cccgcagcca atggtgtcgc aacaagctca acgccccggc
     6121 aaaggtcaaa accgcgccta cggaaagcgt ggtctttcgg gcctgcgcgc ggccgggagc
     6181 ggggttaatc aagctggaat tcctcctgcc aggtcctggc ttccacaaac tcgccctgct
     6241 cccgcttgtc cagaataaag tcaccgagaa ccaggtaatc catttccgtc cgcatgaagc
     6301 acgtgtaggc ctgctcggga gtgcacacga tcggctcgcc acgcacgttg aacgaggtat
     6361 ttaccagcac cggacagccg gtgagccgtt caaaagcgca taacagatcg taatacaacg
     6421 gattttcttc ccgccgcacg gtatggatcc gggccgaata atcgacatgc gttacggcgg
     6481 gaatcgtcga ccggggtaca ttcagctttt cgattccaaa caactgctgc tgctcttggg
     6541 tcatgggaag gcgccggtgt tcacggaccg gcgccaccag taacatgtag ggggaatcgc
     6601 aatccatctc gaaataatcg gccacccgct cgcgcagcac cgaaggagca aaagggcgaa
     6661 agctttcccg aaacttgatc ttaagattca tcaccgactg catgcgcggg gagcgcggat
     6721 cgcccaggat gctgcgcgcg cccagggcgc gcggcccgaa ctccatgcgg ccctgaaacc
     6781 agccgatcac tttctcttcc gccaaaaggg ccgcgacccg ggcgcacagt tccgcacccg
     6841 tagcccgggt gtagcgggcg ccggacgatt cgagaaactc cagaatctcg tccgccgaat
     6901 actccgggcc aagatacgac cctgacatgc cgtccgtgcg gccgcggcaa acagccccgg
     6961 cggaccgggg ttgattatag tactgatacc atacagagag agcggcgccc aaagctccac
     7021 cagcgtctcc cgcggcgggc tggatccaga ggctttcgaa cggcccttcg cggagaatgc
     7081 ggccgttacc cacgcagttt agcgccacac cgccggccag gcaaagattc ctttccccgg
     7141 tctgccggtg cacatggcgc gccatgcgca gcatggcttc ctcggtgacg tcctgcactg
     7201 aacgggccag atccatctcg cgcgcggtca agggcgattc gggccgccgg ggcggaccgc
     7261 cgaagagctc gtggaaggcc tcgctggtca tggtgagacc ctggcaatag ttgaaataac
     7321 gcatgttgag gcggagcgag ccatcctccc gcagatcgac cagttcgtcg agaattttct
     7381 gcgtataccg cggctggccg tagggcgcca gccccatgac tttgtattct ccggaattga
     7441 ccttgaagcc ggtgtaataa gtaaaggcgg aatagagcag gccgagggaa tgcgggaacc
     7501 gcgtctcggc gagcagatca atctgattcc cctcgccgcg gccgtagctg ctggtcgccc
     7561 actcgccgac gccgtcaata gtgaggatag ctgccgattc gaacggcgaa gggaaaaagg
     7621 cgctcgcggc gtgcgactcg tggtgttcgg taaacagcac tttgccttta aagccgcact
     7681 gtctggcgat ctcttcgcgc atccaaagct tctcccgcag ccacaagggc atagcctgaa
     7741 ggaaggaccg caagccgcgc ggggcataat ccagataggt ttcgagcagg cgctcgaact
     7801 tttgcagcgg cttgtcgtaa aaaacgacgg cttccagctc gctggccgaa atgcccccct
     7861 ggcgcaggca aaaagctacg gcatgggctg ggaagcgatg atcgtgcttt ttccgcgaaa
     7921 agcgctcctc ttgcgccgcc gcgacaacgg ctccgtcgcg gacaaggcac gccgctgaat
     7981 cgtgataata ggcggaaagg ccgagaatcc acatcggcgt acggtttatc ttaacatcgg
     8041 ccggcagcgg cccaggttgt tgctagactg tgtgctggat cggccggcgc atcgcgagtc
     8101 ccgcacccgc cgacggcgcc cgggcggctg cgctccgggg ttgcgggagc ttttccggcg
     8161 agtcgcgcga gcgggggcgt gatccaggaa tcgcatgcgc cggcgtccgc caaaaaccac
     8221 catgtgcgga attgcaggca tcttcgggca atcggatgaa catctggcgc ggcagttctg
     8281 ccgcgtgctc gaacaccggg gtccggatga tgaaggattt ctcacgtccg cttcgggccc
     8341 gattacgctg gcaagccgcc gcttggcgat tctcgatctg agcccggccg gccatcagcc
     8401 gatgcaaaca gccggcgggc gctgcacgct ggtctacaac ggcgaggttt acaactttgc
     8461 cgagatccgg agcgagttgc agtcccgcgg gcgccaattc cgcagccgcg gcgacaccga
     8521 agtggtgctc gaagctttgg ccgaaaaagg aaccgcggcg ctcggcgaat ttaacggcat
     8581 gtttgctctg gctttttgga acgcgcgaga gcggaaagga ctgctcgccc gggatccctt
     8641 cggcgtcaaa ccgctttact atgcgcccct gcccggcggg cgcttgattt ttgcctccga
     8701 aatcaaggcg ttgttgctgc atccggagct tgcagcgggc ctgaatctct gcgcccttcc
     8761 gcagtatctg gcctttcttt ggacgccaga cccggcgacg ctcttcgaag gcatttacaa
     8821 actgcctccc ggccatttgc tggagtggca ggaaggagcc ttctcgatcc gccgctatta
     8881 cgagttgccg gcgcttgctt gcagggaaga ggtgagcgaa gaaggagccg tggaaggcct
     8941 gcgcctgcat ctccggcggg ccgtgcagcg gcagcgggtg agcgacgtgc cggtgggatt
     9001 gtttttgagc ggcggcctcg attcgagcgc cttgctggcg gagatggcag gcgcctctcc
     9061 gctcggcccg gcagccaggc aagagattcg atgctacaca gccggctttc ccgcagagga
     9121 caatgccttc gaccagttcg gcagcgaccc gccgtatgcc cgcgccgtgg cccgccgttt
     9181 cggagcgcat cttcgggaat ttgagattcg ggccggcatg acggatttgc tgcccaaact
     9241 gctctatcat ctggacgagc cgctggccga ccctgccgtt attaatacct atttgatttg
     9301 caagcaggcc cgcgaagacg gcgcaagagt gctgctctcc ggacaggggg ctgacgaact
     9361 gctgggcggt tatcgccggc atatcgcgcc gctgctcaac gagcggctgc aaggagcgcc
     9421 ggcggctctg ctgggggccg tggcgcggct gtcgaaacgc ttgccggcgg gccttgcagg
     9481 ccgcgcgggc ggcgctctgc gccgggcgcg caagctgctg gagccggtga atcgcccgcc
     9541 cagcgagcag tttgttcact attgccagtg gctccacgac gatgacgtcc gctcgctcct
     9601 tgcgccggag gtgctcgcga cgctcggctt gtggcggcat ccctcggcgt tcacggagga
     9661 agtgctcggc cagtctccat ccggggggct gctggaccgc atgctctatc gcgatttgaa
     9721 aacgtttctg cccgccctga atctcaccta caccgacaaa atgagcatgg cggcgagcgt
     9781 ggaagcgcgc gtgccgtatc tcgacctcga gctggccgaa ttctgctggc ggctgccgca
     9841 gcgatacaaa gtgcggggcg caaccggcaa gtggctgctg cggcgcgcca tgcgcggaac
     9901 gctgcccgcc gaggtgctgt ggcgctcgaa gacgggtttc ggcgcgccgg tgcggcaatg
     9961 gatgcggcac gaattgaaag aaatggcggc agacctgctc tccccggcag cgctgcgggc
    10021 gggcggcgtt ttttgcgcgg aaggcgtgga agctctgcga gcgagcttcg ccgacgggca
    10081 aggcgaccat ggctatacgt tgtgggcgct gctggtcttt caattgtggc gggaagtctt
    10141 cctgcgacag ccggcggctc gctaacccac ccggcgggcc aaggcaaaga gggaagttcc
    10201 tgtcttcgac tcgccgcgca gcgagcgggt ctggctcagg ccgaaggcgg cgcgcatcag
    10261 cagcggcgcg ggccagggag gcagagccag atagcgtttg taagccgcgg cgggcgcttc
    10321 cgcccttgcg gcgcgcggcc tgcggccccg ctggcgcttg agcaaaggca ccatccagga
    10381 gccccaccag agcatgtgct cgatcactag tcccgttccg gagaaagcct gccggagcga
    10441 ctcgggcaaa tagcggcgcc ggtggccctg taccacgtcg aagtcggaga acagctccgg
    10501 gagggcgggc acgccgacaa tggtaaatcc cccgggcttg gttagttgcg ccagccgggc
    10561 cacagtgccg cgatcgtcat cgagatgctc gattacgtcc aacgccagca cggcatcgta
    10621 ttgctcggca tgcggaggga gcggccgggt caggtcggcc tcgataagct cccggcccgg
    10681 gcggtctagc ttctccaggg accgccgggc gatatccagg cccacagccc gatagcccgc
    10741 gcgttcgagc gcttccaggt tcaggcccca gccgcatccg gcttcgagca cccgcgccgg
    10801 cgggagaaca tttactttct tcagcaaggc caccgccatg cgagcgcgcg cctggtacca
    10861 gggatggcga gaaatggggc ccgccagatc ggtcagaatg tcttcggaca tgccctcact
    10921 ggcattgccg ctggaccatt gaacaatcat gggcgagcaa tccggacaca gccacgcgct
    10981 cgtatgctag ggctcggcat ctgcggctat tctacctgcg aaggctgccg ggaaaaaccc
    11041 agtgagcggc ttacaaccca gcgcgggcgg agcttgactt cgtcgtagat cctcgccagg
    11101 tattccccga tcagccccag aatcagcaat tgaacgcctc cgagaaagaa gaccgaaacg
    11161 atacccgcag cccacccggg gatatcggct gccacgtgcc ccataaattc caaccccgag
    11221 aaccgccaga tcgaaacgag aaggaccacc aaagcgcaca ggccgacgat cgatagcccg
    11281 aaccaggtgg cgagccgcaa aggaaaggtg gaaaacgaga aaattccgtt cagagcgagc
    11341 cgcaggagct tccgaaaggg atatttggtc tcgccggccg cccgcgcgct gcgctcgtag
    11401 gggatgccaa tctgccgaaa gccggcccac gcgcgcatcc cccggacgaa tatgttgcgt
    11461 tcgggcatgc tgcgcagcac attgaccacg ctgcgatcca tgaggcaaaa atcgcccgta
    11521 tcgagaggaa tgtgaatgtc cgcgatcact cgcaggaggc gataaaaaat gctgtaagcg
    11581 gcccgtttca gcggtccttc tttacgtttt tgccggacgg cgtagacaac ctgatagccg
    11641 tctttccagc agcgcaagca ggtggccagc aggtcgggcg ggtcctgcag atcggcgtca
    11701 atgatagcga cagcttcccc gctggcgtag gccaggcctg cggaaatagc ggtttggtgg
    11761 ccgaagttgc gggaaaaact gataacttta aaacgcccat cgcggcggtg catttccgaa
    11821 agaatctcca gcgtgcggtc cgaagagccg tcatccacga acaccagttc ccacgatatg
    11881 ccggctccgc caagcgcctc gagcaaccgc ttttccaaca tcggcaggac cgcttcctcg
    11941 ttgaagcagg ggatcacgac actaagagtc ggccgatcgg gagattcagg catgggtaga
    12001 tacagactcc gcgggctttt gggcggccca cgattcccag cttatccggg cgcgccggta
    12061 aaacccgaag cgataggcca aaaccagccg gatcatgcga acccccaggg agaaggcctt
    12121 ccagaaatcc ccggtgacgg acgaccgccc gacgcggcgg cggtaattca cgggaatctc
    12181 gacgaaagga atctggtgag caatcacttc catcagcagt tgagagccaa aatgagagcc
    12241 gccgatcgcc aaatgcggac ggatcagatc cagagcctcc cggcggaaca atcggagcgt
    12301 gcagcccatg tcggttagaa tggtggtatt gaacaagaac tccgtcagct tcgcgaccgc
    12361 cacgttcccc gacttgagga accagcccat attggcgccc tcccaaatga actcgcgggt
    12421 ggtgcgagtg ccaaaaacca caggcatgtc gtcggagtaa gccagcagct tgatcacgtc
    12481 gtggccgctg aaagtcccat cgggttccgc caggatgatg agctccgccg agcactcgtc
    12541 aatgccgcga agcagcgcgt agccatagcc ctgccggctt tcaaaaacct cgcgagcgcc
    12601 cgtgcctgct atctcctccg aggtacccgg agccgcattg ttatttacga ccagaatttc
    12661 gtccaccagg ccgccggccc agaagtcgag aatggcggag cggatcgaat ccttttcgtt
    12721 ataggtcgga aagacaacgg cgactttctt atcgtgccac atccggatta ttttcgttaa
    12781 cgcggagaac cagaattgta tcggccacac cggcaaaacg ccgcaccacg gaacatcgct
    12841 ccagagaaaa ggcgcgggcc tgccggattt catcctcccg gccggcgcgc gaggcgacgg
    12901 cgagcgggag ggggaaatcc gggtcgagaa acaaacgcag ctccccgctc gccacgatat
    12961 aacgccactt acgcctttgt tgcgcggcta tccagccgga cgcgtccgga ccgcggccgt
    13021 tttccgcctc gagccggcga agatacaagt attcgctgct ggtgaaatct ttgtcccaga
    13081 aagcgaacca aaacacatcg ttgccgagca cgggatcgtc accgggaatc acgcggcgca
    13141 attcggccag gcgcgaggag aaggggacgt cctgccgggc cttccaatgc cacagaccca
    13201 agtttagcgc gcaggcagcc aggagcaagg ccagagcgcc tcgcgcgatc cgccgcgcgg
    13261 gaaaagcctg gaacagacgc gccatccata tagcgaacag cggccagggc agaatcaaat
    13321 accacccaaa gcgattactc atgaaaagga gagcgaccgc gaaaaaagcg ctccaggcgg
    13381 ccaccagggc gcgcccggta cggccctcgc ggagccacaa ccagaccccg gcggccagaa
    13441 tgcaaaggcc ttcgagcgca tgccgatgcc ctcgggcgtc ccaaaaccag tccgtgtagc
    13501 gttgtatttc catcttgaaa gccgcggcca ggccaagatc ggcgatgcgg agcccgtggg
    13561 tctttccgcc gtatacggct gcttgatgga gcatcgcctg cggattgggc cagtaatgca
    13621 gcctcagcca gaaaagcact ccggcggcga cgcccgccga aaaaatagct ccgcccaacc
    13681 agcgcacccg ggccggctct cggcgcagaa acagccaaaa gagaaacggg atgaaggcga
    13741 tcagaaaacc gttcagatgg acgtctcccg agagacccat agccaggccg gcggcagcca
    13801 atttcacata aggctttcca gggcgggcgg aggcggcgag ccacaggctc agcaaaaaaa
    13861 acaatgccag cagcatatcc gggcgcgccc agcggcacgc gtcgaaaaac tttccggagg
    13921 cggccagcag cagcggagca agcaccgcgg tctgccggtc ccacagaacg gcggccagag
    13981 ctgccgtgac cgtcagcacg ccaaggccgg ccagaaacga aggaagcagc gcatggagca
    14041 gggacacgcc gccaaaatgc aagaataggc cctggaaagc agcagccgtg cggccgaagg
    14101 cccacacatc ggctcccaag cccgcggcat cctggtagcc cggcactccc gggcggcccg
    14161 tattccagaa ttggtaggcg gccgaagcat agcagccgtc gtcgtcgtta gggaaggcag
    14221 gcacgcgacc gagagtggcg gcattataca ggagcaggat gccgatggcc gccaaaggcc
    14281 acaaccggcc tgttccgcta tcaattttca ccgccgcgga ttcggcagcg gcctgtttca
    14341 tggttttccc ggaagatgga ggctgtaaat ctgaatcgag cccgctttcc cgtagttgta
    14401 ttcggccagc ggagtcatgt ttcgcgcaag gaacgcccat tgggagggaa gcaaaaattc
    14461 tcggaatacc ttgtccacca cgacaaattg cacctgttgc cggttcaaat atcgcctgaa
    14521 ggcctgctcc cagggaagat cgggcgccca gcgctggacg aaattggccg atacgacact
    14581 gcgcaggttg cggtcgcggg gaaaataaaa ccaaaggttg tccgctgcca aaaacggctt
    14641 atcgcccgca tgccggagaa catcagcccg gacgcggccc ggcaacggca catcgcgatg
    14701 cgccgcccag tagcccatct gcgcgcaata gaacgcgatc agggaaagcc cggcaagaaa
    14761 aaccacgcgg cgagatgatc caaaaaacgc agaagcgatc agaatgctgc cgagcgggta
    14821 gacggcggcc atgtagccgg catggggcag cgcggaaagg atcgccagaa acacgtgaaa
    14881 gagcacgacg attgccgcaa gccgccggac ggggttgcgc cgcatcagac ccgcacatac
    14941 gccggcggca agaaagaagc cctccaaggc ataccggtga taacgcgcat cccaaaaata
    15001 cgagcggtag cgagccacgg cggaggtgat gatggccttg ggtccgagca agaggggcga
    15061 gggtcccgcc gcttgccaga atcccaccca ttgtatgcgc cacagatcgg gctgcgacca
    15121 ggcgtgatcg taaagccaga gaaaagccgc gggagccgcg cccaggcaaa ccagggccga
    15181 attccggatt ctgccggccg gctcggatgt gagcacggcg gcggccagcg cggcgatcag
    15241 ggcggcgccg atcatatgta cttccaagga caaacccgcg atgaacccgg cgagtaaaaa
    15301 aagccgccag tcgtcatttc ggagagcccg cagcaaaagc agacagcaga ccacggtaaa
    15361 gagcatcaga agcggatcgg gccgcgcagt gtgggcgagc atttgcgcgg gaaccgagag
    15421 cagccacagg aggctgcctg tggcggctaa aagttcgggg acgcggcact cccgtgcgat
    15481 gaggagaacc agcggaacgc tgaaccacac cgccagcagg ccaggcagcc gagaggcaaa
    15541 acagttgggg cccaaagcca gctcgcaaag tccgacgagg gccgccggaa tccggccgta
    15601 catgatcaaa tcccggtcga gatcgaacaa acctttatcc atgggcagcc cggcgcggcc
    15661 cgtatggacg aaagaaaaag ctgctgctgc atacgccccg tcatccacag tggccgcgta
    15721 cggaatctgc tcgagcagaa taaggtcaaa cgcgagaatc gccccggcga acgcccagaa
    15781 aaggagagcg agccggcggc gcgtccagtt tgggcgagcg gcgccggcgg cgccaggccc
    15841 ggcctcaaga gagtcacgga agaacgacat tttttaaacg tctgaaatca aatcgttgcg
    15901 cccgggagcc ggccggggcc ccgccggggg gtctattctg agttcgaagc ccgactttgg
    15961 gattcgtgtg gaggagtgat gacgattcga ggactggcct ggcttctgtt tgcaactttg
    16021 ggctgcgcgc agacgcaaat cgatttgacg cggcaggctc gcaaagcttt accttatgcc
    16081 caaggaggca ccaatgcagc cgatcagatc acggcgcgga ggaatgtagg aacgcc
//
LOCUS       DASREK010000330         3283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2466162, whole genome shotgun sequence.
ACCESSION   DASREK010000330 DASREK010000000
VERSION     DASREK010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466162"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFA54_17870"
     CDS             complement(<1..525)
                     /locus_tag="VFA54_17870"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HZS52741.1"
                     /translation="MGKVLVVLGLLGAAIPGWSKSVPQAVEDRYNHLRSLKVEFEESV
                     SYAGQQGTSKTRRQERGTLYLLRPGKMRWEYSDPAGKLFVSDGKMFYLYSPNSNQVQR
                     IKPKQTDDLRAPLAFLLGKLDFQKEFGKITTRNTQEGIELTAQARSPQESYSQAVFTI
                     DPQSYEIRRIRVDGQ"
     gene            complement(679..1965)
                     /locus_tag="VFA54_17875"
     CDS             complement(679..1965)
                     /locus_tag="VFA54_17875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS52742.1"
                     /translation="MMASKATRPPGWARSGWLNRNVIGMAVTSLCSDACYEMVTAVLP
                     GFLAAIGVAATALGWIEGAADALSSFVKLGVGWYSDRIGSRKTIVTVGYFLAGTALSV
                     FALAASWPLVLLGRAVAWLGRGARGPLRDAMLSESVDPRAVGKAFGLHRAADTLGAVI
                     GPLIGVGLLSVLPAASPSEPFRIIFVISLLPGVSAAAVFWATVKELRAPGRRKLGFFA
                     SLRNLPRGYTQLLRGVGLFGLGDFSHTLLILAAGNLLAPYYGVIRAAQIAALLYALRN
                     LLYAGFSFPIGALSDRTPKKGLLAAGYFLGGVTALGAAGLFAFHLHSIPILAALFAAA
                     GIYIAAEDALEGAITADFVPREARGAAYGLLGAVNGAGDLAASALVGTIWTALSPAAA
                     FCCAGFLMLSGAALLAATRPSAYAYLDNRQDEIISG"
     gene            2064..>3283
                     /locus_tag="VFA54_17880"
     CDS             2064..>3283
                     /locus_tag="VFA54_17880"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZS52743.1"
                     /translation="MKEHLRLFSLLLLVCLGRPSKGSAQITVLQNVDLIDGAGGAPKH
                     GVSIVIAGSKIRDIRPTAQFKPPSGANVIDLAGKTIIPGIMNLHGHVGLTKGLTQARE
                     NFTRDSIEAQLRTYASYGVTSVVSMGSDIDPALMLRIRDEQRGGRLNGCARVYSAGRG
                     FTAVGGYPAVLPGNQGVPFEVATSAQARAYVDQLAKMRVDLIKMWVDDELGRYPKLAP
                     ELYTAIIEQAHRHKLKAAAHLFYLADAKRLLDAGIDAMAHSVRDADVDDDLIAKLKRN
                     NATAIPTLSREYSTFAYAQPPSWLDDPFFQRAVAPETIATLKSESYRSRLTSNPDFPR
                     YRGFFETAMRNVKKLHDAGVRIGFGTDTGPPGRFPGFFEHLELELLVQAGLTPMQALE
                     TATRNAAEYLGVSR"
BASE COUNT          667 a         1019 c         1003 g          594 t
ORIGIN      
        1 ctggccgtct acgcgaatcc ggcggatctc gtagctctgc gggtcgatgg tgaaaacggc
       61 ctggctgtaa gactcctgtg gcgagcgggc ttgcgccgtg agttcgatgc cttcctgagt
      121 attgcgagta gttatctttc cgaattcctt ctggaaatcg agtttgccta gcaaaaaggc
      181 gaggggagcg cgaagatcgt cggtttgctt cggcttgatc cgttgcacct ggttggaatt
      241 ggggctgtat aagtaaaaca tcttgccgtc agaaacaaaa agtttgccgg cagggtcgct
      301 gtactcccag cgcatcttgc cagggcggag caggtagagg gtgccgcgct cctgccgccg
      361 agttttggac gttccctgct gcccggcgta ggagactgat tcctcaaatt ctaccttcag
      421 gcttcgcagg tggttgtagc ggtcctctac agcctggggg accgatttac tccagcccgg
      481 aatcgccgcg cctagcagtc ccagcacaac caatactttg cccatcggtt caagcctcaa
      541 gtcattttga cggacggaca tcccctttcg tgtacagacc tcgccgccgg ctcaagatgc
      601 ccggcggatc ttcttcgctg ccatgatagc aggggctgta gtgacgccgg ctctagtccg
      661 gttttgtctg tgccggcttc agcccgagat gatttcgtct tgccggttat ccagatacgc
      721 ataagcgctg ggccgggtgg ctgccagcag ggcagccccg gaaagcatca agaagccggc
      781 gcagcaaaag gcggcggctg gagaaagtgc ggtccatatt gttcctacca gagcgctcgc
      841 cgccagatcg ccggcgccat ttaccgcgcc caacaagcca taagcggcgc cgcgggcttc
      901 tctgggcacg aaatccgcgg tgatggctcc ttccagcgcg tcctcggcgg cgatatagat
      961 gcctgccgcc gcaaacaggg cagcgaggat ggggatgcta tgaaggtgaa atgcaaacaa
     1021 acccgcggcc cccaaggcgg tgacgccgcc cagaaaatac cctgcggcga gcagcccttt
     1081 tttcggcgtc cggtcgctga gagccccgat agggaacgaa aagccggcgt acaacaggtt
     1141 ccgcaaggca taaagcaaag cggcaatctg cgcagcccgt atcacgccgt agtacggcgc
     1201 gaggagattg cccgctgcca ggatgagtag ggtatgggaa aaatctccca ggccgaagag
     1261 gccaacgccg cgcagaagct gtgtgtagcc tcgtgggagg ttccggagcg aagcgaaaaa
     1321 tcccagcttg cgccgcccgg gcgcacggag ctccttcacc gtggcccaaa acaccgcggc
     1381 tgccgagacc ccgggtagaa gggatatcac gaaaatgatt cggaacggct cggagggaga
     1441 tgcggccggc agcacagaca gcaagcccac tccgatcagc ggtccgatca ccgcgccaag
     1501 cgtgtcggct gcccggtgga ggccgaaggc cttgcctact gcccgagggt ctaccgattc
     1561 cgagagcatg gcatcgcgca gcgggcctcg agcgcccctg ccgagccacg caacagcacg
     1621 ccctaacagc accaggggcc aggaagcagc gagggcgaaa accgagagcg ccgtgccggc
     1681 cagaaaatag cccacagtga cgatcgtctt ccggctcccg atccggtcgg agtaccagcc
     1741 caccccaagt ttgacgaagc ttgaaagagc atcggccgct ccctcgatcc agcccagggc
     1801 cgtcgcggcc actccaattg ccgctagaaa gcctggcaac accgccgtca ccatctcgta
     1861 gcacgcatcg gagcacaggc tggtcactgc catgccgatg acgtttcgat tgagccatcc
     1921 ggatcttgcc cagcccggcg gcctggttgc ctttgaggcc atcattcatt atccgcacca
     1981 ttttccatac cgaggcgcaa ccgcggggtc cctgctgccg catttgaagt tacagcccgc
     2041 gcagggtggt atcatacgcc cgtatgaaag agcatctgcg gctgttctcc cttttgcttc
     2101 tggtgtgcct cgggcggccc tcaaaaggat cggctcagat caccgtgttg caaaacgtcg
     2161 atctgatcga cggcgccgga ggcgctccga aacacggcgt ctccatcgtg atcgcgggat
     2221 cgaaaatccg ggatattcgc cccacggctc agttcaagcc gccttccggc gcgaatgtaa
     2281 tcgacctggc cggcaagacg atcattcccg gaatcatgaa cctgcatggg cacgtcggcc
     2341 tcaccaaagg cttgacgcag gcgagagaga acttcactcg cgacagcatc gaagcccaat
     2401 tgcgtaccta cgcttcgtac ggcgtgacta gcgtcgtcag catgggttcc gacatcgatc
     2461 ccgctctgat gctgcgcatc cgggacgagc agcgcggcgg gcgcctcaac ggctgcgcaa
     2521 gagtctattc ggccgggcgc ggcttcacgg ccgtaggcgg atatcctgcc gtgctgccgg
     2581 gcaatcaagg cgtgccgttt gaagtggcga catccgccca ggcgcgagcc tacgtcgatc
     2641 aattggcgaa aatgcgcgtg gatctgatca agatgtgggt ggacgacgaa ctgggccgct
     2701 atcccaagct tgcgccggag ttgtatacgg cgatcatcga acaggcgcac aggcacaagc
     2761 tgaaggcggc cgcacacctt ttttacctgg ctgacgccaa gcgtctgctg gacgcgggaa
     2821 tcgacgcgat ggcgcacagc gtccgcgatg ccgacgtgga tgacgacctg atcgccaagc
     2881 tgaaacgaaa taacgccaca gccataccga ccctctcgcg ggaatactcg accttcgcct
     2941 acgcccagcc gccttcctgg ctggatgatc cttttttcca gcgggcggtt gcgcccgaga
     3001 cgatcgcgac cctgaagagc gagtcctacc ggagccgcct gacgagcaat cccgattttc
     3061 cgcgttaccg gggctttttc gagaccgcta tgcgaaatgt gaagaagctg cacgacgcgg
     3121 gagtgcggat cgggtttggc accgataccg ggccgccggg ccgtttcccc gggtttttcg
     3181 agcacctgga gctggaactg ctggtgcaag caggcctgac gcccatgcag gcgctcgaaa
     3241 cggcgaccag gaacgccgcc gaatacctcg gtgtgtcgcg tga
//
LOCUS       DASREK010000331        13933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Bryobacterales bacterium isolate SMAG_U10004
            k141_2467877, whole genome shotgun sequence.
ACCESSION   DASREK010000331 DASREK010000000
VERSION     DASREK010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963532
            Sequence Read Archive: SRR5581483
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bryobacterales bacterium (soil metagenome)
  ORGANISM  Bryobacterales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Bryobacterales.
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:36:19
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,584
            CDSs (total)                      :: 3,551
            Genes (coding)                    :: 3,540
            CDSs (with protein)               :: 3,540
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 9 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="Bryobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2467877"
                     /isolate="SMAG_U10004"
                     /isolation_source="soil"
                     /db_xref="taxon:2026791"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..863
                     /gene="cofE"
                     /locus_tag="VFA54_17885"
     CDS             111..863
                     /gene="cofE"
                     /locus_tag="VFA54_17885"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZS52744.1"
                     /translation="MSIRVAGLRTLPEIRQGDDLALLIRRAAELENQAIDARTIVVVA
                     QKIVSKAEGAVVDLRQIEPSPAAEHWAALWSEESRQKKDARLIELILRESRRIVKMDR
                     GVLIVETRHGLVAANAGVDQSNLAGEDLASVLPRDPDISAERIRCGLGCGVVIISDTF
                     GRPWREGLVNVAIGVSGMAPLEDYRGAPDRHGRILHGTVIATADEIAAAAGLVMKKAA
                     GVPVALITGMEWKGAAGCGRDLIRPRETDLFR"
     gene            1063..2835
                     /locus_tag="VFA54_17890"
     CDS             1063..2835
                     /locus_tag="VFA54_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS52745.1"
                     /translation="MSTQPVVNATPSAASGLVQIGASPSSIESIIQARLAEERLRLMK
                     EAGIQKKETHHFKRPVERAFTAEERGRTTILFGGLTWKHEKLVHGALEGLGYKCEAVP
                     TPDVAAFQTGKEFGNNGQCNPTYFTVGNLVQYLQRLENQGMSKHDIIDRYVFFTAGAC
                     GPCRFGMYEAEYRLALRNAGFEGFRVLLFQQSGGLNQAEAEAGLEMNLDFFLGILNAL
                     NMGDCINEIAYLIRPYEVRDGETARVLEEVMDYMHDVMAKKQPWKLNGNLAKYLAGFR
                     DTAEYIGKFLDQLKGDDYVSALHKVRDRLNTVEIDRLRVKPIVKITGEFWAQTTEGDG
                     NFNMFAFLEREGAQVLVEPIGTWITYMIHQVVQRYRDHKGLEEGAVLPPVWKLHKRAK
                     IEWAYRNKVAKLRLAEAIFRREYHKIQDALGGAGHRLVDQYELQRIGHPYYNSRTGGG
                     EGHLEIAKNIYYSNKDLAHMVLSLKPFGCMPSTQSDGAQSAVVSHYKDMIYLPIETSG
                     EGEINAHSRVQMALGEAKAKAKQEFASALERAGLTLEQCRAYVEAHPEMRRPLYHVPH
                     HKGVIGTAANFVLHIGERMRAERN"
     gene            3003..6587
                     /locus_tag="VFA54_17895"
     CDS             3003..6587
                     /locus_tag="VFA54_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HZS52746.1"
                     /translation="MDKNFLVGLDVGSTTVKAVVVDQATDQIVWSDYQRHDTKQPEKV
                     LEFLKRFDRELPELRPENTRMFITGSGGNGLARRLGAKFVQEVNAVSLAVEKLYPHCG
                     SVVELGGQDAKIIIFKEDPETGRKKKFPSMNDKCAGGTGAVIDKINAKLRIPPDQLCQ
                     MGYKGLKLHPVAGKCGVFAETDINGLQKQGVPPDELMASLFEAIVMQNLSVLTRGNTL
                     RPVVLLLGGPNTYIKGMQDCWKTNIPKIWEERNYPLPEGVPPEDLIRTPENAQYFAAI
                     GAVEFGKTEDESVGRYQGYRDLEWYINVGREEEKSKRKSSGGGLSKSPEELAAFREKY
                     KRKPFSPAVFQPGEVVEGFIGIDGGSTSTKAVLLSKDKDRRILAKAYQLSRGNPIEDT
                     QEVLGKLVRMVEDQGAALKILGVGTTGYAKDILKDVIGADAALVETVAHTQAALKFYP
                     QADVICDVGGQDIKIIILKDGRVKDFKLNTQCSAGNGYFLQSTAEGFSIPVEKYADVA
                     FSATSFPSFGYGCAVFMQSDIVDFQRQGWKAEEIMAGLANVLPKNIWLYVSQIPNLSA
                     VGSFFVLQGGTQYNLAAVKAQVDFIESRFKGRDVKPTVVVHEHAGEAGAIGCALEAGR
                     LWDNGRQSSFIGMAGVNTISYKTTRNEATRCYFCKNKCLRTFIDVKTATPNESYRPPV
                     KTKVPIEAGSQRLIIATCEKGTVENIEDMRGIKAGLDKVKSENPNLVEVAAKAVFRST
                     RAPLVADPIPRFAFTAAQKNRVELMKKRAEIKIGMPRVLNMYSQTPVFMGYFESLGLQ
                     PENLVFSDYTSEQLYKEGAKRGAIDPCFPSKVAIPHIHNLLYVIHPKRKLDYIFFPMI
                     DCLTSDLVNTQGSRACPTVAATPAAVKAAFTKEGDIFKEKGVVFLDTFVNIGKPEVLE
                     RQLYTQFKDILGLSPEENHRAVQEGYRALDYYNNVVQRGQGREVLEMLEREDKIGIVL
                     LGRPYHNDPGINHEIVEEFQKLGYPVFAQDSLPLDDDILWRLFGEEVERGEITHPMDI
                     QDVWKNSYSENTSRKVWAAKYVARHPNLVALELSSFKCGHDAPIYTVVEEIVEHSGTP
                     YFSFKDIDENKPTGSIKIRVETIHYFLKRYREDMIANKGKEKTIEEKLREFEARLRRE
                     LMQQTVSPEVFARGHRETIHVSLDALAASNPAASGLAHHEHHEMAAGD"
     gene            6761..7282
                     /locus_tag="VFA54_17900"
     CDS             6761..7282
                     /locus_tag="VFA54_17900"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HZS52747.1"
                     /translation="MQRLLLAFRAFAGILFHGTISEPLAAALGLGRRTSGHPSPAAAP
                     ASRPADGALQVLGILQRDSRLIDFLMEDLSAYSDDQVGAAVRSLHDDCRKSLARYVQL
                     APVIDGVEGSFTSPNIPEPGAVKFVGNVPAQGLPKGGILRHKGWRATRVDFPALPPGQ
                     DASIVAPAEIEIE"
     gene            7289..9112
                     /locus_tag="VFA54_17905"
     CDS             7289..9112
                     /locus_tag="VFA54_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZS52748.1"
                     /translation="MPQHVIGIDLGTTNCSLAFADPAAITDPHQLPEVKLFSIPQLVN
                     PGEVQEETVLPSFLFLPGALDFPPGSLALPWNPDPPYVAGRLAQNRGVENAARLVHSA
                     KSWLCHTGVDRSAAILPSNAPDGVNKISPIEASRAYLDHLRCAWEWKRPDAPFDQQQV
                     LLTVPASFDAVARELTLRAAKLAGYQDVILLEEPQAAFYAWIERHPDWRERIKVGDLI
                     LVADIGGGTTDFTLITVTEENGELKLERVAVGDHILLGGDNMDLALARFTEQQLAAKN
                     VRLDALQLHALWQHCRVAKEKLLEEQTTAADYPLTILGRGSGLVGGTIRAKLARKDLE
                     QILIEGFFPITPSTDTPQRQRRVGLQEAGLPYAADPAVTRHLARFLRQQAAASEHGAV
                     RRGPSGLACPTHILFNGGVMRAAQLRERLTAVLDGWLREEGFASVQPLLGEDLMHAVA
                     RGAAYYGLARQGKGVRIRGGVARTYYVGIESAMPSVPGVPAPLKALAVAPFGMEEGTQ
                     VQIPNREFGLVVGEPAEFRFFASSSRKNDPPGSLIEELGEDLEELSPMEVCLPGENHN
                     ELVPVTLETVVTEIGLLQLWCVARDGRRWKLEFNVREHIAA"
     gene            9109..11778
                     /locus_tag="VFA54_17910"
     CDS             9109..11778
                     /locus_tag="VFA54_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZS52749.1"
                     /translation="MKIGIDLGTTNSALAFIDPQEAENSSSPPIRIQPIPQVVAPGVV
                     EPRKTLPSFLYVADQTYVGAYARDQGALEPTRLVHSAKSWLSNPEVDRTAKILPWDSE
                     ESGRIFSPVEVSARFLSHIRDAWNQAHAEPLENQDIVLTVPASFDEEARELTVAAAKE
                     AGLESLTLLEEPAAAFYSWIANHLGQCRQSLADGQTVLVVDVGGGTSDFTLIRVSRDK
                     DVVSFTRTAVGRHLLLGGDNLDLTLAWLVEAKLGAQLSLRQRNSVRRQCTIAKERLLT
                     DPSVDKVNITVLGAGGSLVGGSLKTEILRQEALELALEGFLPFCSPADTPKEERRTLF
                     RELGLPYVSDPAITRHLAAFLHGAGMSGAPDAILFNGGFFIPEICRQRLADVIEGWYG
                     RRPMILENQDLDLAVAVGAAYYSYARSTGCGVLVRGGLPRAYYLGLDGADAAKSARMV
                     CLAPRGSESGETLYWEHEDLQLVANRPVTFRLYSSLTRTGDALGDVLTLSPEQVAADF
                     HVHAPLHAVIRFGKRAGERLVPIRLGVRLTEVGTLEIWADSKVSDHRWRLQFELRKQA
                     EMAERAASRPATVINDEALARALDVVRSGLSADPPPFPPEEFPARLEQALGLGKMSWP
                     LSAIRKLADALLELAEGRKRSGAWEARWLNLCGLCLRPGFGYPGDDWRIEQIRRIYAA
                     GIQFPNHVQCEIEWWIFWGRVAGGLNRNQQIDLYHHLAPVLLPASKKRRRVNTSLLRE
                     MWRTASSLELLPSQTRIDLGNALLRRVQTGDFRESELWCLSRLGARELLYGPANQVLS
                     PAVVSRWVEVLLPLPEAAEAVASIARRTGDPVRDLSPSLLEVVRRALAERTNPERLLA
                     VLEGEEVRDPLLLSRIFGEELPSGLVFRAAAGK"
     gene            complement(11831..12988)
                     /locus_tag="VFA54_17915"
     CDS             complement(11831..12988)
                     /locus_tag="VFA54_17915"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZS52750.1"
                     /translation="MFGRRIRLFKLLGFEVRADASWLIIAALVTWSLGAGFFPYYDPG
                     LTTSDYWWMGVAGAAGLFGSIIVHELFHSLVARRFNLQMRGITLFIFGGVAEMEKEPP
                     SAKAEFSMAIAGPITSIVIGFIFYGIHEAAAGAWPRPVAGVLAYLAGINWLLAAFNLI
                     PAFPLDGGRVLRSILWYRQGDLARATRISSSIGSGFGIVLMILAVVQLFTGNFISAVW
                     WFLIGMFLRGASQASYQQLMIRSILAGEPVRRFMRTNPVTVSPDATIQELVEDYVYKH
                     HYKMFPVVDSEQELVGCVTTSQIKNIPRQQWPWRHVQEIVQPCSAGNTVSPDTDAMNV
                     LTTMSKTGFSRLMVVENNHLEAVITLKDLLNFLSTKLELEGYPQRDIPRVA"
     gene            13488..>13933
                     /locus_tag="VFA54_17920"
     CDS             13488..>13933
                     /locus_tag="VFA54_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZS52751.1"
                     /translation="MEPRARQLLERSEPQTPPAPRQPPKTTAKPRRGVAWLLLLAVAA
                     AAYAGFQYYRTAQGKKQAAAAALAARAAARAVPVAAVPARAGDMPVYLRGLGNVTAFN
                     SVTVRTRVDGQLMSVDFQEGQHVNKGDLLAEIDPRPFQVQLEQAEG"
BASE COUNT         2766 a         4101 c         4424 g         2642 t
ORIGIN      
        1 cgagaattgc atccgccagg gctggcgttg gacatcgaca cgcccgagga catcgcggag
       61 ctgctggcgc gggccccgga atcaaagatt gcgagacttc tcgaggggaa atgtccatcc
      121 gggtcgcggg cttaagaacg ctgccggaaa tccggcaggg agatgatctg gcgctgctga
      181 ttcgccgcgc agcggaattg gaaaatcagg ccatcgacgc gcggacgatc gtggttgtag
      241 cgcagaagat cgtttcgaag gccgaggggg cggtggtaga tttgcggcag atagagcctt
      301 cgccggcggc ggagcattgg gccgcgctgt ggagcgagga gagccgccag aagaaggatg
      361 cccggttgat cgagctgatt ctgcgcgagt cccggcgcat cgtgaaaatg gaccgcggcg
      421 tgctgatcgt cgagacgcgc cacggcttgg ttgcggccaa cgcgggggtc gatcagtcga
      481 atctggcggg cgaggatttg gcttccgtgc tgccgcgcga tccggatatt tctgcggagc
      541 gcatccgctg cggccttggc tgcggagtag tgatcatcag cgataccttc gggcggccgt
      601 ggcgggaagg cctggtcaat gtcgctatcg gggtttcggg catggcgccg ctggaggatt
      661 accggggcgc cccggaccgc cacggacgaa ttctgcatgg cacggtgatt gctacggcgg
      721 atgagattgc tgctgcggcg ggcttggtga tgaaaaaagc cgccggcgtg cccgtggcgc
      781 ttattacagg gatggagtgg aagggggcgg cgggttgcgg ccgtgacttg atacgtcccc
      841 gggaaaccga cctttttcgc taagcgccgc gaggctccgc ctctgccacg tgttgtaagc
      901 ctaattaatt ctccgggtta cgtgaaaaaa cgtccaccgc tcgggccgcg ccctcaccgt
      961 tcaaggaagt aatttcacca ttcggtggtt tcccgttttc caccggcttg tcctggtgca
     1021 ctatggccgg gaaagacgcg agtagtgtcc tccgaggtga atatgagcac ccaacccgtc
     1081 gttaacgcca cgccttctgc cgccagcggg ctggtgcaaa ttggcgcatc cccttcctcg
     1141 atcgaatcga tcatccaggc gcggctggcg gaggagcggc tgcgcttgat gaaagaagcc
     1201 gggattcaaa aaaaagaaac gcatcatttc aagcggcccg tggagcgcgc gttcaccgcc
     1261 gaagagcggg gccggacgac gattttgttc gggggcctga cttggaagca cgagaagctg
     1321 gtgcacggag ccctggaggg cctgggttac aagtgcgagg cggtgccgac gccggacgtg
     1381 gccgccttcc agaccggaaa ggagttcggc aacaacggcc aatgcaatcc tacgtatttt
     1441 acggtcggca acctcgtgca gtacctccag cgcctggaaa accagggtat gtccaagcac
     1501 gacataatcg accgttacgt tttcttcacc gccggcgctt gcgggccctg ccggttcggc
     1561 atgtacgagg cggaataccg gctggccctg cgcaacgcag gattcgaggg tttccgcgtg
     1621 ctcctgttcc agcaatccgg cggcttgaac caggccgagg cggaagccgg cctggagatg
     1681 aatctcgatt tctttttggg cattttgaac gccctgaaca tgggcgattg catcaatgaa
     1741 atcgcttacc ttatccgtcc ttatgaagtc cgcgacggcg agacggcgcg cgtgctcgag
     1801 gaagtgatgg attacatgca tgacgtgatg gccaagaagc agccgtggaa gctgaatgga
     1861 aacctggcca aatacctggc gggcttcaga gatacggcgg aatatatagg aaagttcctg
     1921 gaccagctca agggcgacga ctatgtatca gcactgcata aggtgcggga ccggctgaac
     1981 acggttgaaa ttgaccggct ccgggtcaag ccgatcgtga agatcaccgg cgaattttgg
     2041 gcgcagacca ccgaaggcga cggcaatttc aatatgttcg ctttcctcga gcgggaaggc
     2101 gcgcaggtgc tggtcgagcc gatcgggacc tggatcacct acatgatcca tcaagtggtg
     2161 cagcgctacc gcgaccacaa gggcctggag gagggcgcgg tgcttccgcc cgtgtggaaa
     2221 ctgcacaagc gcgccaagat cgaatgggcc tatcgcaaca aggtggcgaa gctgcgcctg
     2281 gcggaggcga tttttcgccg cgaatatcac aagattcagg acgccttggg cggcgccggc
     2341 caccggctgg tcgatcaata cgagttgcag cgcatcggcc acccatatta caactcccgc
     2401 accggtgggg gcgagggcca tctggagatc gccaaaaata tttactactc gaacaaagat
     2461 ctggcgcaca tggtgctttc gctgaaaccc ttcggctgca tgccctccac gcaatccgac
     2521 ggcgcgcagt cggccgtggt gtcgcattac aaggacatga tctatttgcc cattgaaacc
     2581 tcaggcgagg gagagatcaa cgctcacagc cgggtgcaga tggcgttggg agaggcgaaa
     2641 gcaaaagcga aacaggagtt tgcgtccgcg ctcgagcggg caggccttac gctcgagcaa
     2701 tgccgggctt acgtcgaggc gcacccggag atgaggcggc ctctgtatca cgtgccgcac
     2761 cacaaaggcg tgatcggcac ggccgccaac ttcgtgctgc atatcggcga gcgtatgcgg
     2821 gcggaaagga actgaagcgc cccgccggcg caagataggg tggcgccggc ggcgtcggtt
     2881 tcgtcgtttg ccacggagaa aaccgtttcc cgaggccgcg aagccggaag cagcagcggc
     2941 ccgcgggccg ggaaacgatt ggaatggaag cgcagccgca aaccagcggc tctgagcggg
     3001 aaatggacaa aaactttctg gtgggactcg acgttggctc cacgacggtg aaggctgttg
     3061 tcgtcgatca agcgacggat cagattgtct ggagcgacta ccagcgccac gacaccaagc
     3121 agccggaaaa ggtgctggag ttcctcaagc gctttgaccg cgaattaccc gagctgcgtc
     3181 ccgaaaacac acggatgttc atcaccggct cgggcgggaa cgggctcgcc aggcggctgg
     3241 gcgcaaaatt tgtgcaggaa gtgaacgccg tttcgctcgc cgtggagaag ctctacccgc
     3301 actgcggctc ggtggtcgag ctgggcgggc aggacgccaa gatcatcatc ttcaaggaag
     3361 atccggaaac cggccgcaag aagaagtttc cgtcgatgaa cgacaaatgc gcgggaggca
     3421 ccggcgcggt gatcgacaag attaacgcaa agctgcgcat tcctcccgac cagctctgcc
     3481 agatgggcta caaggggctc aagctgcacc cggtggccgg caagtgcggc gttttcgccg
     3541 agaccgatat caacgggctg caaaagcagg gcgtgccgcc ggacgagttg atggcctcgc
     3601 tgtttgaggc catcgtcatg cagaacctgt cggttctcac gcgcggcaac acgctgcgtc
     3661 cggtggtcct tttattgggt ggccctaata cctatatcaa gggcatgcag gattgctgga
     3721 agacaaacat cccgaaaata tgggaagagc ggaactatcc gctgccggag ggcgtgcctc
     3781 cggaggacct tattcggacc cccgagaatg cgcaatactt cgccgctatc ggcgcggtgg
     3841 agttcggcaa gacggaagat gagagcgtgg ggcgctacca gggctaccgg gatctggagt
     3901 ggtacatcaa cgtcgggcgg gaggaggaga aatccaagcg caaaagctcc ggcggcgggt
     3961 tatcgaagtc ccctgaggag ctcgcggcat tccgggagaa atacaagcgc aagccctttt
     4021 ccccggccgt gtttcagcca ggcgaggtgg tggaaggatt tatcggcatc gacggcggct
     4081 cgacgtccac caaggcagtg ctgctgtcga aagacaaaga ccgccgcatt ctggccaaag
     4141 cgtatcagct ttcccgcggc aatccgattg aggacacgca ggaagtgttg ggcaagctgg
     4201 tgaggatggt ggaagaccag ggggctgcgc tcaagattct cggcgtcggc acgaccggct
     4261 acgcgaaaga cattttgaag gacgtgatcg gagccgacgc cgctttggtg gagaccgtcg
     4321 cccacaccca ggcggcgctg aaattctatc ctcaggccga cgtgatctgc gacgtgggcg
     4381 gccaggacat caaaatcatc attctaaaag acggccgggt caaagatttc aagctgaata
     4441 cgcaatgctc ggcgggcaac gggtacttcc tgcaatcgac ggccgaaggc ttcagtatac
     4501 cggttgagaa atacgcggat gtcgccttca gcgccacgtc gtttccctcg ttcgggtatg
     4561 gctgcgccgt gttcatgcag tccgatatcg tcgattttca gagacagggc tggaaggcgg
     4621 aagagatcat ggccgggctc gccaacgtgc tgcccaagaa catatggctc tatgtttccc
     4681 agatacccaa tctttcggcc gtggggagct tctttgtcct gcaaggcggc acccagtaca
     4741 atctcgccgc ggtcaaggcc caggtggatt ttatcgagtc gcgtttcaag ggccgcgacg
     4801 taaagcccac cgttgtggtg catgagcacg cgggagaagc cggggccatc ggctgtgcgc
     4861 tcgaagcggg caggttgtgg gacaacggcc gccagtcgag cttcatcgga atggccggcg
     4921 tcaataccat ttcctacaaa accacgcgca acgaagccac gcggtgctat ttctgcaaga
     4981 acaaatgcct gcggacgttt atcgacgtca agacggccac gcccaatgaa agctaccgcc
     5041 cgccggtgaa aaccaaagtg ccgatcgagg ccggctcgca gcgcctgatt atcgccacct
     5101 gtgagaaggg cacggtggaa aacatcgaag atatgcgcgg catcaaggcg ggccttgaca
     5161 aggtgaaaag cgaaaatccc aacctggtcg aggttgcggc caaggcggtg ttccggtcca
     5221 cgcgggcgcc gctggtggcg gatccgattc cgcggttcgc tttcaccgcc gcacaaaaga
     5281 atcgcgtgga gctcatgaag aaacgggctg agatcaagat cgggatgcca cgcgtgctca
     5341 acatgtactc gcagaccccg gtcttcatgg gctattttga gtcgctgggc ttgcagccgg
     5401 agaatctggt gttttccgac tacacgagcg agcaactcta taaagaaggg gccaagcgcg
     5461 gcgccatcga tccgtgcttt ccatccaagg tcgccatccc gcacattcac aatctcctgt
     5521 acgtgatcca cccgaaaagg aagctcgact acatcttctt tcccatgatc gactgcctga
     5581 cctccgacct ggtgaacacg caggggagcc gcgcttgtcc caccgtggcc gccacgccgg
     5641 cggcggtaaa agcggctttc accaaagagg gagacatctt caaagagaag ggcgtcgttt
     5701 ttctggacac ctttgtgaac atcggcaaac ccgaggtttt ggaacggcag ctttatactc
     5761 agttcaaaga cattctcggg ctgtcccccg aagagaatca ccgggctgtt caggagggat
     5821 accgggcgct cgactactac aacaatgtcg tgcagcgggg ccagggcagg gaggtgctcg
     5881 agatgctcga gcgggaggac aagatcggca ttgtgctgct gggccgcccg tatcacaacg
     5941 atccgggcat caaccacgag attgtggaag aattccagaa gctcggctat ccggtttttg
     6001 ctcaggactc gctgccgctc gatgacgaca tactctggcg cctgttcggc gaagaagtcg
     6061 agcgcggcga aatcacgcac ccgatggaca tccaggacgt ctggaagaac tcgtattcgg
     6121 aaaatacgag ccgcaaggtc tgggccgcca aatacgtcgc gcgccatccg aatctggtcg
     6181 cgctggagct ttccagcttc aagtgcgggc atgacgcgcc catctacacg gtggtcgagg
     6241 agatcgtcga gcactccggc acgccctatt tctccttcaa ggacatcgat gaaaacaagc
     6301 cgaccggctc catcaagatc cgggtggaga cgatccatta tttcctcaag cggtatcgcg
     6361 aggatatgat cgccaacaag ggcaaggaaa agacaatcga agaaaagttg agggaattcg
     6421 aagcacgcct ccgccgcgag ttgatgcaac aaactgtctc gccggaggtc ttcgcccgcg
     6481 gccaccggga gaccattcac gtatccctcg acgcccttgc cgcatcgaat cccgccgctt
     6541 cgggcctggc gcaccacgag catcacgaga tggcggccgg cgactgaatc cagggcggaa
     6601 aagtactccg ccccgcggcg cggcgcccga aaacaggcga ttggctcttt tcaaagccgg
     6661 cggcggaagc cggaggccgg ctcgaatcgt tcgctggcgc acaatccaac tcccggcggt
     6721 ctttccacat gcgttgtatc ctgaatattt cggaggaatc gtgcaacgcc ttctgctggc
     6781 ctttcgggcc ttcgcaggca tcctgtttca cgggactatt tccgagccgt tggcagcggc
     6841 gctgggtctg gggaggagaa catccggcca cccttcgccg gcagccgccc cggcaagccg
     6901 gcccgctgac ggcgccttgc aggttttagg catcctccag cgcgattcgc gcctgatcga
     6961 ttttctcatg gaagatcttt ctgcctattc ggacgaccaa gtgggagccg cggttcgcag
     7021 cctgcacgat gactgccgga agtcgctcgc ccgttatgtt cagcttgcgc cggttatcga
     7081 cggcgtcgag ggaagcttca ccagtcccaa tattcctgag ccgggggcgg tcaagttcgt
     7141 cggaaacgtt cccgcccagg ggctgccgaa aggcggcatc ttgcgccata agggctggcg
     7201 ggcgaccagg gttgattttc ccgccctgcc tcccggccag gacgcttcca ttgtcgctcc
     7261 cgccgagatt gagatcgagt agaccgcctt gccgcaacat gtaattggaa tcgacctggg
     7321 aaccaccaat tgcagcctgg ctttcgccga tccggcggca atcacggatc cgcatcaact
     7381 tcctgaggta aagctgttca gcatcccgca gctcgtgaac cccggagagg ttcaggagga
     7441 aaccgtcctt ccgtcctttc tgttcctgcc cggcgctctg gattttcccc ctggttcgct
     7501 ggccttgccg tggaatcccg atccgcctta tgtggccgga cgtctcgcgc aaaatcgcgg
     7561 agtggaaaac gcggcccgtc tggtgcactc ggccaagtcg tggctttgcc acacgggggt
     7621 tgaccgcagc gcggccattc tgccctcgaa cgctccggac ggggtgaaca agatttctcc
     7681 tatcgaagcc tctcgcgcct atctcgatca tcttcgctgc gcctgggaat ggaaaaggcc
     7741 tgacgctcct ttcgatcagc aacaggtgct gcttacagtg cccgcttcct ttgacgctgt
     7801 ggcccgggaa ctcacgctgc gggcggctaa gctcgccggc taccaggatg taattctgct
     7861 cgaggagccg caggccgcct tttatgcgtg gatcgaacgg catccggact ggcgcgagcg
     7921 tatcaaggtc ggcgacctga tcctagtggc tgacatcggc ggcggcacaa cggatttcac
     7981 gctcattacg gtcaccgaag agaacggcga actgaagctc gaacgcgtcg ccgtgggcga
     8041 ccacatcctg ctcgggggcg acaacatgga tctggcgctg gcccgcttca ccgagcagca
     8101 gctcgcggcg aaaaacgtgc ggctcgatgc gctccagctc cacgcgcttt ggcagcactg
     8161 ccgggtggcg aaggaaaagc tgctggagga acagaccacg gcggcggatt atccgctgac
     8221 tatccttggc cgcggaagcg gcctggtggg aggaacgatc cgggcgaagc ttgcgcgaaa
     8281 ggatctcgag cagattctaa tcgaaggatt tttccctata acgcccagca cggatacgcc
     8341 gcagcgccag aggcgcgtcg ggttgcagga agccggcctg ccttacgcgg cagacccggc
     8401 ggtgacgcgc catctggcgc gttttctgcg ccagcaagcg gctgcatccg aacacggggc
     8461 ggtgcggcgc gggcccagcg ggcttgcctg ccctacgcat attcttttca acggcggcgt
     8521 gatgcgcgcc gcgcagctcc gggagcggct cacagccgtt cttgacggct ggctgcgcga
     8581 ggaaggtttt gcctccgtcc agccgctttt gggcgaagac ctgatgcatg ccgtggcgcg
     8641 gggagcggct tattatggcc tcgcgcggca aggaaagggc gtgcggatcc ggggcggggt
     8701 ggcccgcacg tactacgtcg ggattgaaag cgcgatgccg tccgtgcccg gtgttcccgc
     8761 accactgaag gcccttgccg tggctccgtt cggcatggaa gaaggcacac aggttcagat
     8821 tcccaatcgt gaattcggcc ttgttgtcgg cgagcccgcc gagtttcgat ttttcgcttc
     8881 ctcgtcgcgg aagaacgacc cgccgggctc gttaatcgag gagctcgggg aagatttaga
     8941 agagctctct cccatggagg tttgcctgcc gggggaaaac cataatgagc tggtcccggt
     9001 cacgctcgag accgtggtga cggaaatcgg tcttctccag ttgtggtgtg tggcgcggga
     9061 tggccggcgc tggaagcttg aattcaacgt ccgggaacac atcgccgcat gaaaattggg
     9121 atcgacctgg gaactaccaa ctcggctctg gcattcatcg acccgcaaga ggccgagaat
     9181 tcgagttctc cgcccattcg cattcagccc attccccagg tcgtcgcgcc tggggtcgtt
     9241 gagccgcgca aaacgcttcc ttcctttctt tatgtggccg accagaccta tgtcggagcg
     9301 tacgcccgcg atcagggagc gctggagccg acccggctgg tccactcggc gaaatcgtgg
     9361 ctttcgaatc cggaggtcga tcgcaccgcc aaaattctgc cgtgggactc tgaggagagc
     9421 ggccgcattt tctctcccgt ggaggtgtct gcccgatttc tgagccatat tcgcgacgcc
     9481 tggaaccagg cccacgcgga gcctttggaa aaccaggaca ttgtcctcac tgttcccgcg
     9541 tcattcgatg aagaggcgcg cgagctcacc gtagcggcgg caaaggaagc gggcctcgaa
     9601 tcgctgaccc tgctggagga gccggccgcc gccttctatt cctggatcgc caatcatctc
     9661 ggccagtgcc gccagagcct tgccgacggc caaacagtat tggtcgtgga tgtgggaggg
     9721 ggaacctccg atttcactct gatccgcgta tcgcgtgaca aagatgttgt gagctttacg
     9781 cggaccgccg tgggccgcca cttgctgctg ggcggggaca atcttgattt gaccctggcc
     9841 tggctcgtcg aggccaagct tggggctcag ctcagcctgc gccagcggaa ttccgttcgc
     9901 cggcagtgca cgattgcaaa ggaacggttg cttaccgatc cgagtgtcga caaggtcaat
     9961 atcacagtgc tcggcgcggg cggatctctg gttggcggat cgctgaaaac ggaaatcctc
    10021 cgtcaggaag ccctggaact cgcgttggaa ggtttcttgc ccttttgctc gccggcggat
    10081 accccaaaag aggaacggcg cacgctgttt cgcgaactgg ggctgcccta cgtatcggat
    10141 cccgcaatca cgcggcatct ggcggcattt ctgcatgggg ccgggatgtc cggcgcgccg
    10201 gacgccatcc tcttcaacgg cggttttttc attccggaaa tctgccggca gcgcctggcg
    10261 gatgtcatcg agggctggta tgggcggcgg ccgatgattt tggagaacca ggacctcgat
    10321 ctggcggtcg ccgtgggagc cgcctattat tcctatgcac ggtccacggg ctgcggcgtg
    10381 ctcgtgcgcg gcggcttgcc gcgcgcctat tatctcggtc tggacggcgc cgatgccgcg
    10441 aagagcgcgc gaatggtatg cctggcgccg cggggtagcg aatccgggga gaccctgtat
    10501 tgggagcacg aggatttaca gcttgtggct aaccgcccgg tgacgtttcg tctttacagc
    10561 tctctcactc gcaccggcga tgccctgggc gatgtgttaa ctttgagtcc cgagcaggtg
    10621 gccgccgatt ttcacgtcca tgcccccctg cacgcagtca tccgcttcgg caagcgcgcg
    10681 ggcgagcggc tcgttcccat ccggttggga gtccggctga ccgaggtggg aacgctggaa
    10741 atctgggccg actccaaggt gagcgatcac cgctggcgat tgcagtttga gcttcgcaag
    10801 caggcggaaa tggcggaacg agccgcttcg cgcccggcca cggtgatcaa cgatgaggcg
    10861 ctcgcccgcg cccttgatgt agtgcgctcc ggcttgagcg ccgatccgcc gccctttccc
    10921 cccgaggagt ttcccgccag actcgagcag gctctggggt tgggaaagat gtcttggccg
    10981 ttgagtgcta ttcgcaagct ggcggatgcc cttctcgagc tcgcggaggg gcgtaagcga
    11041 tcaggggcgt gggaggcgcg ctggctcaat ttgtgtgggc tctgcctgcg cccaggtttc
    11101 ggctatcccg gcgatgattg gagaatcgag cagatccggc ggatctacgc cgcagggatc
    11161 caatttccca atcacgtcca gtgcgagatc gagtggtgga tcttctgggg ccgcgtggcg
    11221 ggcgggctca atcgcaacca gcagatcgat ttataccacc atctggcgcc tgtgctgctg
    11281 cctgcctcga aaaagaggcg gcgggtgaac acgagcctcc tccgcgaaat gtggcgtacg
    11341 gcgagcagcc tggaactgct gccttcccaa acccgcatcg atctcggcaa tgccctgctg
    11401 cggcgggtcc agaccggcga ttttcgcgag agcgagcttt ggtgtttgtc ccggctgggc
    11461 gcgcgcgaac tgctctatgg tccggccaac caggtgctct ccccggccgt ggtgtcgcgt
    11521 tgggtggagg tgttgctccc tctaccagag gcggcggaag cggttgcgtc gattgcgcgc
    11581 cgcacggggg acccggtgcg cgatctctct ccttccctgc tggaggtcgt ccgccgggct
    11641 ctcgctgaga gaaccaatcc ggagcgcctg ctcgccgtgc tggaaggtga ggaagtccgc
    11701 gatcccttgc tgttgagccg gattttcggt gaggaactgc cgtcagggct ggtcttcaga
    11761 gccgccgccg gcaagtgatc tgccgaactt tcggcgcctg gaaattacta agacactgga
    11821 ttgatttagc tcaggcgacc cggggaatgt ccctttgcgg atagccttcc agttcgagct
    11881 tggtcgaaag aaaattcagc aggtctttca gggtaatcac cgcctccagg tgattgttct
    11941 ccaccaccat caggcggcta aatccggtct tgctcatcgt ggtaagcacg ttcatcgcgt
    12001 cggtatcggg gctgaccgta ttgccggccg aacaaggctg gacgatttct tgaacatggc
    12061 gccagggcca ttgctgccgg gggatgttct tgatctgtga cgtcgtaacg catcccacca
    12121 actcctgttc gctgtctact accgggaaca ttttgtaatg gtgcttgtaa acatagtctt
    12181 cgaccagctc ttgaatggtc gcgtcgggcg ataccgtaac cgggttggtt ctcatgaacc
    12241 ggcgcaccgg ctcgccggcg agaatgcttc gaatcataag ctgctggtat gacgcctggg
    12301 acgctccacg caagaacata ccgatcagaa accaccaaac cgcgctgatg aagttgccgg
    12361 tgaacaactg aaccacggca agaatcatca atacgatccc gaatccggag ccgatcgaag
    12421 agctgatgcg cgtggcccgg gccagatcgc cttggcgata ccaaagtatg gaccgcaaaa
    12481 cgcggccgcc gtcgagggga aacgcgggga tgaggttgaa tgcggcgagc agccagttga
    12541 tcccagcaag atatgcgagc acacccgcca ccggccgggg ccaggcgcca gccgccgcct
    12601 catggattcc gtaaaagatg aacccgatca cgatgctggt gatcggcccg gcgatcgcca
    12661 tggaaaactc ggctttcgcg ctgggcggct ctttctccat ttcggccacg ccgccgaaaa
    12721 taaagagggt gatgcctctc atctgcaagt tgaaccgtct ggccaccaga gagtggaaca
    12781 gctcatgcac aatgatggac ccgaacagtc ccgctgcgcc cgccacgccc atccaccagt
    12841 aatctgaggt ggtgagcccc gggtcatagt atgggaaaaa accggcgccg agcgaccagg
    12901 tcaccagcgc ggcgatgatg agccaacttg catcggcgcg cacctcgaaa ccgagaagct
    12961 tgaacaagcg aattcgccgc ccgaacatag gggctctcct ctcttttcgg ggtgaccggg
    13021 cgcgcgagag agcagcaccg cgaggccaag ccccggggtg tccttttctt cattgtacgc
    13081 tgactgctag attgtccgct gacttctaga gacctgatgt ccgcggccac gaagcgacgg
    13141 gaatccccca cagagcgcgg cggctcttgc gtacgccctg gcttcgcagg attcaaccta
    13201 catcggccgc cgggcagatc gcatgcggcg cggatggcgc catctcctgc tttcaacccc
    13261 gcatccgaag cgaattgcct ccgggcgcaa gcgcacagac ccgcaatcgc tttgtgttcg
    13321 gcggagtttt ggaattgcag gcgtcttttc cgttcaccag aagacccatt ctcgaggaac
    13381 gagtgaactc tgtaacaccg ccgcgcgcgc ccgcgtctcc ataattgtgg ggcggcgggt
    13441 ttgggcgcca tgccggcgct aaagagcctg ccgtgcggaa atatgtcatg gagcctcgcg
    13501 cgcgtcagct tctagagcga agcgaaccgc aaacgccccc ggcgcccagg caacctccga
    13561 agaccactgc caagcctcgc cggggcgtcg cctggctgtt gctccttgcg gtggccgctg
    13621 cggcatatgc gggatttcag tattaccgga cggcgcaggg gaaaaagcaa gccgccgcgg
    13681 ccgcgctggc ggcgcgagcg gctgcgcggg cagttcccgt cgccgctgtt cctgccagag
    13741 ccggcgacat gccggtgtat ctgaggggcc tcggcaatgt caccgccttc aactcggtaa
    13801 cagtgcggac gcgcgttgac gggcagttga tgagcgtaga tttccaggaa ggccagcatg
    13861 tcaacaaagg cgacttgctc gccgaaattg atccgcgtcc cttccaggtg cagctcgaac
    13921 aagcggaagg gca
//
